LOCUS       DAOQMM010000001       100912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_22_length_100912_cov_57.656722, whole genome shotgun sequence.
ACCESSION   DAOQMM010000001 DAOQMM010000000
VERSION     DAOQMM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 100912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 100912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100912
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22_length_100912_cov_57.656722"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLH85_00005"
     CDS             complement(<1..508)
                     /locus_tag="PLH85_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNB62968.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEAGKPKVIENSEGARTTPSIVAYAD
                     DGEILVGAPAKRQAVTNARNTLYAVKRLIGRRFEEKEVQKDIDLMPFTIAKADNGDAW
                     VEVRGKKIAPPQVSAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRII"
     gene            complement(604..1188)
                     /gene="grpE"
                     /locus_tag="PLH85_00010"
     CDS             complement(604..1188)
                     /gene="grpE"
                     /locus_tag="PLH85_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNB62969.1"
                     /translation="MQDQTTPPTPDHAPELVQGSDLPPAAPDAPEAPVDVVPGLEQAL
                     RQAELKAAEHHDAWLRARAETENVRRRAQDDIAKAGKFAAEKFAQAMLPVKDSLEAAL
                     ASENATLESLRQGVELTLKQLLSAFQGASVTELNPLGEKFDPNRHQAISAIEADGEPN
                     TVINVLQKGYLLHDRVLRPALVVVSKARSAQPAA"
     gene            1344..2705
                     /locus_tag="PLH85_00015"
     CDS             1344..2705
                     /locus_tag="PLH85_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB62970.1"
                     /translation="MDKAPDKVGKYKLLREIGRGATATVYLAEHPQYPDPVALKLIRF
                     DDKSKDEAKWNRRLLKLLRAEMDVSRRLDHPNIIKIFDTAVDKDFAYIVMEYFPGTTL
                     EHYCSFERLLPLHRTIGMVFKCCMALDYAYHQGIVHRDIKPANILVDENDNVKITDFG
                     LALNISKKSASDSTFIMGVGSPAYMSPEQIKGYPLNQKTDLFSLGVVLFHMLTGRLPF
                     RAKNPAQLIYKIINAEPPLVSQINPEIPEQMDAVIRKSLEKDLYSRYKNGADFAKDLS
                     AVRYKIFDDSYVPPDNRRFSQLRKMSFFLEFDDVEIWEVLRISRWRRVDSNTLLMREG
                     DADQRFGIIVDGKVELSVDGHRVCELGDGELFGELAFLDQQDHKHMYTVVSTTSLTYL
                     EMNPAALSLATEECQDAVRSKVVTVAARRLGNSMRELSKDGPPAERITASGFGMELQL
                     VDS"
     gene            complement(2737..3171)
                     /gene="trxC"
                     /locus_tag="PLH85_00020"
     CDS             complement(2737..3171)
                     /gene="trxC"
                     /locus_tag="PLH85_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNB62971.1"
                     /translation="MPDPILVPCPHCGAINRLPGERLRDQGRCGRCKQALFTGEPLHL
                     TGEDFWIHAGRGEIPLVVDFWAPWCGPCRSMEASFAAAARRLEPAYRLGKVNTEDEPD
                     LATRFGIRSIPTLVLMRGGREVARRSGAIGADELVRWLTSVS"
     gene            3336..3842
                     /locus_tag="PLH85_00025"
     CDS             3336..3842
                     /locus_tag="PLH85_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HNB62972.1"
                     /translation="MDNDTSRTHGWLVTIQRRGVIYRKHFSDGVFGGKQRSFSAAKEY
                     RDQIIAAHPPLSMQEYSNIVKKNNRSGVVGVCRYCASETRDLPEEKQRWFWVASWPLP
                     DGRRKRVKFSVKKYGEDGAFRMAMDARAMALEKLEGAFDPGAVRRKSSRRRTTPVPAT
                     GGAPASRN"
     gene            complement(3867..4208)
                     /locus_tag="PLH85_00030"
     CDS             complement(3867..4208)
                     /locus_tag="PLH85_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNB62973.1"
                     /translation="MKLILRWFLNAVALMLIPEVVTGIHVDSYSAALISAVLLGFVNA
                     ILRPLLLLITLPITVLTLGLFALVINGLMFWLVSGLVQGFTVTDFGTAFLGALLYSLL
                     TWLVNLALGGD"
     gene            complement(4242..5339)
                     /gene="hemH"
                     /locus_tag="PLH85_00035"
     CDS             complement(4242..5339)
                     /gene="hemH"
                     /locus_tag="PLH85_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNB62974.1"
                     /translation="MTRFWQEPPFRHGSPSRTGILIVNLGTPAAPTADALRPYLREFL
                     SDPRVVEIPRPIWWLILNGIILNTRPAKSARKYASVWTDEGSPLKVHTERQAKLLAGF
                     FVADGAPDLVVNWAMRYGQPSVRSRLDEMRAQGCTRVLVIPMYPQYAASTTASVLDAV
                     GDCLQHWRNLPEIRYVRSFADDPGYIGALAASVREHWQRNGQAEKLVLSFHGIPRRSL
                     DLGDPYHCECHKTARLLGEALKLPAERLVISFQSRFGRAKWLEPYTQATLEALARDGV
                     TSVDVMCPGFTADCLETLEEINMECRDAFLAAGGKAFGYIPCLNERDDWIRALRTIAR
                     RHLGHWLDLPAEDPARLAASAERARQLGATI"
     gene            complement(5349..6377)
                     /gene="hrcA"
                     /locus_tag="PLH85_00040"
     CDS             complement(5349..6377)
                     /gene="hrcA"
                     /locus_tag="PLH85_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNB62975.1"
                     /translation="MSSLDQRSQILLKTLVEHYIADGQPVGSRALSRFSGLQLSPATV
                     RNVMSDLEELGFITSPHTSAGRIPTARGYRFFVDSLLKVSPVGSGQIETLRDQIQPGE
                     PQRVINAASQLLSDLTHFAGVVISPRQESTRIRQIEFLRLSEGRILLIVVTATGDVQN
                     RILFTKRSYSPSELVTAANYLNQHFAGLSFDEIRIRIQDELKQLRSDMTELMTAAVEA
                     GSAAVAEHQQTYVISGETNLLDAEDLSSNMARLRELFKLFEQKTGLMQLLDISNRAQG
                     VQIFIGSEAGLSPLDDCSVVTAPYEVDGQLVGSVGVIGPTRMAYERVIPIVDITARLL
                     TNALSGQN"
     gene            6567..7451
                     /locus_tag="PLH85_00045"
     CDS             6567..7451
                     /locus_tag="PLH85_00045"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNB62976.1"
                     /translation="MAHAFDTIALIGKYQSPEVAEAVLRLANYLRGLGLPVIIEQGTA
                     SATGLTSGFTVGSYEEIGVRADLAVVLGGDGTLLHSACRLAGYDVPLVGVNQGRLGFL
                     TDISRDVALDKIGEILAGRFTEEHRVLLDVEVLRGGQRVFRTTVLNDVVVNKGELGRM
                     IEFDLRVDGEFVYTQRSDGMIVSTPTGSTAYALSANGPILHPSVDGIALVPMCPHALT
                     ARPITLPDRCCIEIVLLPPHDSRVHFDGQSRYDARAGDTVRITRSPHTVRLLHPVGYS
                     YFAMLREKLHWSSTPRHS"
     gene            7471..9141
                     /gene="recN"
                     /locus_tag="PLH85_00050"
     CDS             7471..9141
                     /gene="recN"
                     /locus_tag="PLH85_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNB62977.1"
                     /translation="MLRHLTVRDFVIVDHLELEFCAGFGALTGETGAGKSILLDALGL
                     ALGGKGDAGVVRSGQARAEIVAEFDLPADSALAAWLEEQSLTSEDGTVQMRRVIDSSG
                     RSKAWLNGSPVVLGQLRAAGELLADIHGQHAHHALLRSEAQLALVDAHGGLSADAVEV
                     ARRFRVWMGARKAREASEAGAASSERERELLTWQVTELRELAFEPDEWDALNQEQARL
                     AHAASLIEGSGEILDGLAEGELALVSLLERLAGRAADMRQYDARVGDVEDLLRGAGIQ
                     LDEAVHALRRYRDRLDLDPQRLTEVDQRIQAVTALARKHRCAPADLPALLAESDARLA
                     QLTRQSDPEALAREEAAAQEAFLAVARPLSVARKQVASALSAAVTEAMQTLAMAGARF
                     EVCLATEVAGAATGLERVEFLVTANASQPLRPLSKVASGGELSRIGLAIQVITSRNAA
                     TPTLLFDEVDVGIGGRVAEIVGRLLRQLGSDRQVLCVTHLPQVAAQANWQWSIAKDND
                     GDETLSRVMVLTPTERIEEIARMLGGVQITDTTRSHAAEMLAAAGNPV"
     gene            complement(9162..9740)
                     /locus_tag="PLH85_00055"
     CDS             complement(9162..9740)
                     /locus_tag="PLH85_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNB62978.1"
                     /translation="MEAFLTSAALVALAEIGDKTQLLSFVLAARLRKPWAIVAGIFVA
                     TILNHALAGSVGVWLANLIPAHWLPWIVGSVFVVFGLWTLKPDTLDDDEAPAAMHPAG
                     AFMTTLIAFFMAEMGDKTQLATVALAARFDALGWVVLGTTIGMMIANVPAVLLGEALA
                     RRLPLTQIRWAAAAVFILTGLVTFAGAAGLAG"
     gene            complement(9770..10222)
                     /gene="fur"
                     /locus_tag="PLH85_00060"
     CDS             complement(9770..10222)
                     /gene="fur"
                     /locus_tag="PLH85_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNB62979.1"
                     /translation="MSSNSQNLKNIGLKATTPRLKILDLFQKADNRHLTAEDVYRLLI
                     AEDMDIGLATVYRVLTQFEQAGLLERHYFESGKAVFELNEGHHHDHLVCLQCGKVEEF
                     YDPEIEERQNRIARERGYEVHEHALYLYVDCKKTDCPHRPGKSGTPLI"
     gene            10334..10690
                     /locus_tag="PLH85_00065"
     CDS             10334..10690
                     /locus_tag="PLH85_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNB62980.1"
                     /translation="MRPLLCLLLVLPTLGGCSAVIDFLKPYRIDVRQGNFVTQESVSQ
                     LKPGMSRDQVRFLLGTPLLNDVFHTDRWDYVYRLQPGKGEAQERRLTVVFEDGKLVRL
                     EGDVRAAGGEGTATRP"
     gene            10739..11545
                     /gene="dapB"
                     /locus_tag="PLH85_00070"
     CDS             10739..11545
                     /gene="dapB"
                     /locus_tag="PLH85_00070"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNB62981.1"
                     /translation="MSQVRIAIAGASGRMGRMLIETTLKAADMKLVAAFEQLGSPFVG
                     KDAGELVGQACGVAISCDVAAGLAEADCLIDFTRPEGTLHHLALARAARCAMVIGTTG
                     FGPEGKAAIGAAAQDIPVVFAPNMAVGVNAVFKLLDVAARILNEGYDVEIIEAHHRLK
                     VDAPSGTALRMGEVVASALGRNLEECAIYGREGVTGERDSQTIGFATIRGGDVVGDHT
                     VLFAGIGERIEITHKSSSRMSYATGSLRAARFLAGKDSGLFDMQDVLGLR"
     gene            11676..14279
                     /locus_tag="PLH85_00075"
     CDS             11676..14279
                     /locus_tag="PLH85_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB62982.1"
                     /translation="MNLPSLPPPASSDELVLALRVLLQDLASRGCITMPSAASEANPA
                     ELVDGVRALIAAKQRIEQQYHETRERLDLAMQNTDAGLWDWDLAHDTIVLGEHWRQIL
                     GYAPGEGSADFDGWLHRVHPDDLQVMRHQLAAHLRGDLEDFDAQFRIATNATAWRWIQ
                     VRGRASNRNAAGRWCRLVGTFRDITRVKEAELELLDAKEMAEAANRTKSDFLANMSHE
                     IRTPMNGIIGMTDLVLDTDLDPVQRDYLLAVKNSADTLLHIINDVLDFSKIEAGRMSL
                     EQIDFSVVELMGATMKSLALTAYQKGIECLYRVAPAVPAVVVGDPTRVRQVLTNLVGN
                     AIKFTERGEVVVSVDGESLDGNEFLLRVAVRDTGIGIPADKQEQVFGAFSQADESTTR
                     KYGGTGLGLAISRRIVQAMGGDLSLESEEGRGCVFSFTVRLGRGVQANGRPPPVFAGR
                     RALVAAANGAVRELLRAELERAGVTVREAADGEALAAQLKSAQDTAKPFDWLIMEAAM
                     SAPGGFALAEQLRKGWPTLDRLVVVSDPKSQRSDADRCEKLRIGARLIKPFSAGDLSE
                     ALSLALHVDRLEQVDYLEFQGEQTAWESAPRIAERLAVLLVEDNPVNQVVAVKLLEKA
                     GHTVTVAVNGREALAQYDKRRFDVIFMDIQMPEMGGIEATQAIRAREARRSWAAGRQS
                     LSVPIIAMTAHAMQGDKEACLMAGMDDFVTKPIRPPELYAAIARVRQAQSFAASDEHL
                     ADAGLLNGDAAIEPEEIADLGATRDLLDGDEDQLQNLIRIFFNDFDRNRKALDAALAT
                     RDFARLRDVAHTLKGSAGVFSASRVVASAAQVEKAARAADEAECRRCVNELMRELGVL
                     AGALRRARRGG"
     gene            14450..15598
                     /gene="carA"
                     /locus_tag="PLH85_00080"
     CDS             14450..15598
                     /gene="carA"
                     /locus_tag="PLH85_00080"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNB62983.1"
                     /translation="MSAFPPAILALADGTVFYGKGIGAVGMTTGEVVFNTSMSGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGINQEDCEAGRVYAAGLVIRDLPLLASNWRNEQSLDA
                     YLKAENIVAIAGIDTRKLTRILREKGAQGGCIVTAATTAELSSEAALAEARAFPGLAG
                     MDLAKVVSAAESYAWTEGEWQLDKGYVPQEAPRFHVVAFDYGVKRNILRMLAERGCRL
                     TVVPAQTPAAEVLALNPDGVFLSNGPGDPEPCDYAIAAIREVLAKGIPTFGICLGHQL
                     LALASGARTLKMKFGHHGANHPVKDLDSGQVLITSQNHGFAVDVATLPANLRATHVSL
                     FDGSLQGLARTDVPAFCFQGHPEASPGPHDVAYLFDRFIALMEGRRAS"
     gene            15665..18889
                     /gene="carB"
                     /locus_tag="PLH85_00085"
     CDS             15665..18889
                     /gene="carB"
                     /locus_tag="PLH85_00085"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNB62984.1"
                     /translation="MPKRTDIQTILIIGAGPIIIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPGTADVTYIEPITWQVVERIIEKERPDAVLPTMGGQTALNCALDL
                     ARHGVLEKYGVELIGAKEEAIDKAEDRQKFKAAMTRIGLGSARSGIAHALEEAHQVQA
                     EIGFPVIIRPSFTLGGTGGGIAYNTEEFVEICKRGLEASPTNELLIEESLIGWKEYEM
                     EVVRDKADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQILRNASIAVLREIG
                     VDTGGSNVQFAINPKDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LKNDITGGATPASFEPSIDYVVTKIPRFAFEKFPLANDRLTTQMKSVGEVMAMGRTFQ
                     ESFQKALRGLETGAYGLDEVEADQDDLERELGDPGAQRIWYVGQAFREGMSLDQVHAL
                     TRIDPWFLVQIEDIVKTEKTLAGRSLKGISAAELRELKRKGFSDRRLAKLLATDETSV
                     RLHRHTAGVRPVFKRVDTCAAEFATSTAYLYSTYEEECEAQPTGRKKIMVLGGGPNRI
                     GQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDILEIV
                     AVEKPVGVIVQFGGQTPLKRARELEANGVPIIGTSPDMIDAAEDRERFQKLLFELGLK
                     QPPNRTARTPEDAVRLAAEIGYPLVVRPSYVLGGRAMEIVHEQKDLERYMREAVKVSN
                     DSPVLLDRFLNDATEVDVDALSDGHRVIIGGIMEHIEQAGVHSGDSACSLPPYTLSAK
                     LQDELRRQTEAMARALNVVGLMNVQFAIQGEGDDAVVYVLEVNPRASRTVPFVSKACS
                     LPLAKIAARCMAGRSLDDQGVSGEVVPPYYAVKEAVFPFVKFPGVDTILGPEMKSTGE
                     VMGVGRTFAEAFVKSQLGAGVRLPTAGKAFISVKHTDRPKAVEVARELVDLGFSVVAT
                     RGTAGAIAAAGLPVSAVNKVNEGRPHVVDMIKNNEIALVINTVEEKRQAVTDSRSIRT
                     SALAAKVTVYTTIEGARAACMGMRHLSGLEAYSLQALHAELSQA"
     gene            18886..19362
                     /gene="greA"
                     /locus_tag="PLH85_00090"
     CDS             18886..19362
                     /gene="greA"
                     /locus_tag="PLH85_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNB62985.1"
                     /translation="MSKIPLTVAGAEKLRQELHRLKTVDRPNVIAAIAEARSHGDLSE
                     NAEYDAAKERQGFIEGRIREVEGKLGNAQIIDPTLLDADGRCVFGATVDLEDLDSGAK
                     VSYQIVGDDEADIKEGKIAISSPIARALIGKFAGDVAEVQAPGGVRELELIDVRYV"
     gene            19415..19855
                     /locus_tag="PLH85_00095"
     CDS             19415..19855
                     /locus_tag="PLH85_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNB62986.1"
                     /translation="MYSLLITLWVGALWTVGYMVAPTLFHMLDDRALAGRLAGQLFTY
                     VGWLGMASAAYLLVFVALRKNGAAFKSSVFWLVLVMLLLTVAGAFGIQPVLAQLKAES
                     LPREVMEGAVRDRFVAWHGVSSVVYLVESVLGVLLVVVQGRGLR"
     gene            complement(19876..20328)
                     /locus_tag="PLH85_00100"
     CDS             complement(19876..20328)
                     /locus_tag="PLH85_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNB62987.1"
                     /translation="MMIELTPAQRRAFRAQAHHLHPVVSVASNGLTPAVLNEVDAALQ
                     AHELIKIRVYGEDRAQRDAILATVCVELGAAAVQHIGKTLVVWRERREDEKTVAPVTD
                     KPRRSLAKAKSAKVLSARRGVSSLKPKSAQKKPYWFASPSDRKPRRED"
     gene            20365..20985
                     /gene="rlmE"
                     /locus_tag="PLH85_00105"
     CDS             20365..20985
                     /gene="rlmE"
                     /locus_tag="PLH85_00105"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNB62988.1"
                     /translation="MKRSKTSKSWMMEHVNDPFVQRAKTEGMRARAAYKLMEIDEKDK
                     LFRSGMTVVDLGAAPGSWSQVAVGKVGGAGRVFALDLLPMEPIPGVHFMQGDFHDDAF
                     VEAFEVQLGDGQVDLVLSDIAPNISGIPSSDQARSIHLAELALDFAARHLAANGRFLV
                     KVFQGEGFDDYRKAMGEAFRSVVVRKPQASRGRSAEVYLLGTGKRS"
     gene            21102..22991
                     /gene="ftsH"
                     /locus_tag="PLH85_00110"
     CDS             21102..22991
                     /gene="ftsH"
                     /locus_tag="PLH85_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNB62989.1"
                     /translation="MNNLFKNLAIWLVIGVVLMTVFNQFNNRQTAQNSVEYSQFMEDA
                     KQGKISKAVVDGRTVRATTQDGRVVTVYTPGVQDIWMVGDLMRYGVKVTASKPEEEQS
                     FLMNIFVSWFPMILLIGVWVFFMRQMQGGGRGGAFSFGKSKAKMLDESANTVTFADVA
                     GCDEAKEEVGELVDFLRDPSKFQKLGGHIPKGVLMVGSPGTGKTLLAKAIAGEAKVPF
                     FSISGSDFVEMFVGVGAARVRDMFEQAKKQAPCIIFIDEIDAVGRQRGAGLGGGNDER
                     EQTLNQLLVEMDGFEGVSGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQ
                     ILRVHMRKVPIAPDVDALVLARGCPGFAGADLANLVNEAALFAARGSKRLVDMDDFER
                     AKDKIMMGAERRSMVMPEEERRNTAYHESGHAVVAKCLTKTDPVHKVTIIPRGRALGV
                     TMQLPEQDRYSQDRERLLQTIAVLFGGRIAEEIFMNQMTTGASNDFQRATDLARRMVT
                     QWGMSDNLGPMVYGEEEGEVFLGRSVTTHKNMSEATLQKVDAEIRRILDQQYALARRL
                     IEENREKVEAMTTALLEMETIDSDQINDIMDGRTPRPPKPSQVPRRPSDDAPGAAPTA
                     PATAA"
     gene            23113..23919
                     /gene="folP"
                     /locus_tag="PLH85_00115"
     CDS             23113..23919
                     /gene="folP"
                     /locus_tag="PLH85_00115"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB62990.1"
                     /translation="MAIVNVTEDSFSGDGCKAFDHVLRAAERAVEQGADLIDVGGESS
                     RPGAAGVSEAEELERVVPVIAALQGLGVPLSVDTVKPRVMKAALDAGADLINDIAAFR
                     QPGALEAVKDSSAALCVMHMQGEPRTMQIEPVYIDVVDEVRIFLDERVKALLQAGVAA
                     ERILVDPGFGFGKTLEHNLALLARLEVFGKSGFPVLVGMSRKSMLGALTGRPVGERQV
                     AGAVAAMWAIQRGARIVRTHDVAATRDVIRVFSGLEAAEFRMGRHCEPSD"
     gene            23928..25283
                     /gene="glmM"
                     /locus_tag="PLH85_00120"
     CDS             23928..25283
                     /gene="glmM"
                     /locus_tag="PLH85_00120"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNB62991.1"
                     /translation="MSRRYFGTDGVRGRVGELPITPDFGLRLGYAAGKTLVAHENLRP
                     GEHPAVIIGKDTRISGYMLESALEAGFSAAGVDVYLAGPLPTPAIAYLTRALRLQVGV
                     VISASHNPYDDNGIKFFSASGTKLPDAVEQEIEARLDEPMGCESSAALGKARRIDDAR
                     GRYVEFCKSTFPNELDLRGMKIAVDCAHGAAYMVAPKVFHELGAEVIATGVEPNGLNI
                     NEGVGATSPTHLRDTVLASGADIGIALDGDGDRIMMADSAGNLYDGDKLLYVIAKAAL
                     VAGSPGAGVVGTLMSNLGFEHAIARLGLPFARAKVGDRYVLEQMHERRWRLGGENSGH
                     IICLDKHTTGDGIVAGLQVLAALRKQGLTLEQMCEDLEFYPQKLVNVPLRSGFVWTDS
                     REIARAVDDAKATLGQSGRVLLRPSGTEPLLRVMVEGQDARIVEQLAHGIAEVVRAAA
                     M"
     gene            25399..26178
                     /gene="tpiA"
                     /locus_tag="PLH85_00125"
     CDS             25399..26178
                     /gene="tpiA"
                     /locus_tag="PLH85_00125"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNB62992.1"
                     /translation="MRKKLVVGNWKMNGGLGQNASLLSSLRQAEALAGVDVAVCVPFP
                     YLSQAQQMLSQSSVAWGAQDLNMHDSGAFTGEVSGGMLRDFSCSWVIVGHSERRALFG
                     EGDDVVGRKVRAALRVGLSPILCVGEALAERRSGVAESVVKRQLSVLAGLSADELERV
                     VVAYEPIWAIGTGVTASPEEVRSMHRLIRQWLGSMHGEHVVDKLRILYGGSVTAENAA
                     LLFSQDDVDGGLVGGASLKFDSFYAVCQAASVGTGSGEICG"
     gene            26171..26536
                     /gene="secG"
                     /locus_tag="PLH85_00130"
     CDS             26171..26536
                     /gene="secG"
                     /locus_tag="PLH85_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNB62993.1"
                     /translation="MGNMMFSLVLVVHVLAGLGVIGLVLVQHGKGADMGAAFGSGASG
                     SLFGSSGSANFLSRTTGVLAAVFFVTSLGLSYLAGGAERKQPTSVMEGVVHPPVSGGV
                     ATPPPPPIEDASKAKDIPR"
     gene            26599..26683
                     /locus_tag="PLH85_00135"
     tRNA            26599..26683
                     /locus_tag="PLH85_00135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26633..26635,aa:Leu,seq:gag)
     gene            26776..27150
                     /gene="ndhC"
                     /locus_tag="PLH85_00140"
     CDS             26776..27150
                     /gene="ndhC"
                     /locus_tag="PLH85_00140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNB62994.1"
                     /translation="MLEAYFPVLVFILFAAGFGLIPIFAGWILGPSKPDAEKLSPFEC
                     GFEPFEDARIKFDVRYYLLAILFILFDLEVAFLFPWATILREIAGTEAVRSFGFFEML
                     VFLGILLVGYIYVWKKGALDWE"
     gene            27214..27690
                     /locus_tag="PLH85_00145"
     CDS             27214..27690
                     /locus_tag="PLH85_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNB62995.1"
                     /translation="MSIEGVFKEGFVTTSLDTVINWTRTGSLWPMTFGLACCAVEMIH
                     AGCSRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMSEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIVQLQNKIKRECTIAR"
     gene            27703..28308
                     /locus_tag="PLH85_00150"
     CDS             27703..28308
                     /locus_tag="PLH85_00150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNB62996.1"
                     /translation="MSSKLENLTRCLSEKFADDIVRLIDAAGEVTLEVRSENYLAVAE
                     SLRDDPELAFEQLVDLTGVDYSVHALADSFSGRFAVVSHLLSIRHNVRLRLRTFAEDS
                     DFPMVASLCAVWPSANWFEREAFDLFGVMFMGHPDLRRILTDYGFVGHPFRKDFPVSG
                     FVEMRYDQDLGRVIYQPVSIEPRENTPRIVREDSYGDVGHG"
     gene            28301..29554
                     /locus_tag="PLH85_00155"
     CDS             28301..29554
                     /locus_tag="PLH85_00155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNB62997.1"
                     /translation="MAEIRNYTINFGPQHPSAHGVLRLVLELDGEVVERADPHIGLLH
                     RGTEKLAETRTWIQSVPYMDRLDYVSMMCNEHAYCMAIERLLGLEVPLRAQYIRVMYD
                     EITRILNHLLAVGTHALDIGAMTMVLYTFREREDLMDAYEAVSGARMHAAYYRPGGVY
                     RDLPDRMPQYEVSKWKSEKIVRELNENRQGSLLDFLEDFCERFPRYVDEYETLLTDNR
                     IWKQRTVGIGVVSPEQALALGFTGVMLRGSGIEWDLRKKQPYEVYSQMDFDIPIGTAG
                     DCYDRYLCRIEEMRQSNRIVKQCIEWLRKNPGPVISDNHKVAPPAREQMKANMEELIH
                     HFKLFTEGIHVPAGEVYAPVEHPKGEFGVYAISDGANKPYRLKLRSPGFAHLSAMDAM
                     SRGHMIADVVAIIGTMDIVFGDVDR"
     gene            29617..30093
                     /gene="nuoE"
                     /locus_tag="PLH85_00160"
     CDS             29617..30093
                     /gene="nuoE"
                     /locus_tag="PLH85_00160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNB62998.1"
                     /translation="MLSPESLKKIDREIAKYPVDQKQSACMSALRIAQMEKGWLAKET
                     IEFVARYLDMPSIAAYEVATFYNMYDLTPVGAKKITICTNLPCVLSGGVHAAEYLKKK
                     LGIDFNETTADGLFTLKEGECMGACGDAPVLLLNNHRMCSWMTEEKIDQLLADLKK"
     gene            30090..31409
                     /gene="nuoF"
                     /locus_tag="PLH85_00165"
     CDS             30090..31409
                     /gene="nuoF"
                     /locus_tag="PLH85_00165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNB62999.1"
                     /translation="MSAQGIILSGLDGDRTWRLTDYLKRGGYEALKKIIADKIPADQI
                     IAEVKKSALRGRGGAGFPTGLKWSFMPRAFPGAKYIACNSDEGEPGTFKDRDILRFNP
                     HSVIEGMVIAGYAMGCERGYNYIHGEIFETYERFEEALQEARDAGLLGKNILGSDFSF
                     ELFAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASI
                     PYIIREGGDAFLALGKPNNGGTKLFSVSGHVNRPGNYEIPLGTPFPVLLEMAGGMRGG
                     RKLKGVIPGGSSAPVLPGHVMMDCTMDYDSISKAGSMLGSGAVIVMDESTCMVKSLER
                     LSYFYFEESCGQCTPCREGTGWLYRVVHRIENGLGRPDDLDLLNSVTANIMGRTICAL
                     GDAASMPVQSFIKHFGDEFAYHIEHKKCLVPVDVQREGSKIFVGSTC"
     gene            31403..33751
                     /gene="nuoG"
                     /locus_tag="PLH85_00170"
     CDS             31403..33751
                     /gene="nuoG"
                     /locus_tag="PLH85_00170"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNB63000.1"
                     /translation="MLEIEIDGKQLQVPDGSTVIEAASKVGAYVPHFCYHKKLSIAAN
                     CRMCLVQVEKAPKPLPACATPVTNGMKVWTHSEPAIKAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLSVGYGGVSSRYQEEKRVVSNKDLGSLVSTDMTRCINCTRCVRFTTEIA
                     GLMELGQAFRGEHAEIMPFVEKALESELSGNIIDLCPVGALTSKPFRFGARAWEMSRR
                     KSVSPHDSLGSNLIVQIKNDKVKRVLPLENEAINECWLSDRDRFSYEAVNGPERLLKP
                     MIKQGGVWQEATWEVALEYVVNGLKSVSKEHGPKSIGAWISPHSTLEEMYLAQRLMRG
                     LESGNVDFRLRQSDFSADDKLEGAPWLGMSIADVSKLDRLLIVGSFFRKDHPLLAQRV
                     RQAAKKGLKINALGAAFDDWRLPLANVCKVAPDEFKVVLARIAGRIASQTSQSISDEL
                     ASFVSESGADNESSAIADSIASGRKIAIWLGNQAAQDVESVQLQILIQEIARLSRASF
                     GVIGEAANSVGGYIAGAIPHADLAGMNVQQMINGDLKACVLLGVEPEYDMADGVAART
                     ALQNAETVIVLNAFKSKDALDYADVMLPVAPFTETAGTYVNAEGAIQSFYSVVKSTGD
                     SRPAWKILRVLGSMAGIPGFEFERIEDVRDASLGPNHANVKLSNVLKGCVVTAKRKAC
                     SLQRVSDIPPYAADPIVRRSEPLQNTLDAVIPTARMSSKTLAEIGVGDGEKVRVKSKS
                     GEVTIFAQVDSGVADGCVRLAGAHDATKLLGSLSAEILVERA"
     gene            33751..34800
                     /gene="nuoH"
                     /locus_tag="PLH85_00175"
     CDS             33751..34800
                     /gene="nuoH"
                     /locus_tag="PLH85_00175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNB63001.1"
                     /translation="MENVLQPVAEFFGDVWPLVWSLAKIVGIIAPLFLCVAYLTLAER
                     KVIGYMQVRIGPNRVGPKGLLQPIADGVKLLLKEIIVPSCASKSLFIVGPVLALGPAL
                     AAWAVVPFADGWVVGNVDAGLLFLLAITSIEVYGVIIAAWASNSKYAFLGGMRAAAQM
                     VSYEISMGFALVSVLMISSSLNLTDIVSSQGKGQWADWGFGFLSWNWLPLFPMFLVYL
                     ISGVAETNRAPFDVVEGESEIVAGHMIEYSGMAFALFFLGEYANMILISALTSILFLG
                     GWLSPFGFLPDSFVWLALKTSFILFLFLWFRATFPRFRYDHIMRLGWKVFIPVTLFWV
                     MLVAVWIMSPLSVWK"
     gene            34811..35299
                     /gene="nuoI"
                     /locus_tag="PLH85_00180"
     CDS             34811..35299
                     /gene="nuoI"
                     /locus_tag="PLH85_00180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNB63002.1"
                     /translation="MGSMKDFVGSLFLKELLKGMALTGRHLFARKITVQFPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESEQRGDGSRRTSRYDIDLTKC
                     IFCGFCEEACPVDAVVETRIFEYHGEKRGDLYYTKQMLLAVGEKHEKQIAADREVESK
                     YR"
     gene            35330..35929
                     /locus_tag="PLH85_00185"
     CDS             35330..35929
                     /locus_tag="PLH85_00185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNB63003.1"
                     /translation="MDFQVVVFYFFSAIMVIASLKVITSRNPVHAALYLVLSFVSASG
                     IWLLLQAEFLAIALVMVYVGAVMVLFLFVVMMLDINIERLRQGFWSYLPVGALIAIAM
                     VVEMGMVLGTYFGLDSVPLPPAPEQGYSNTKALGRLLFTEYAYPFELASIVLLVAMIA
                     AVALTLRKRDSVKKVDPSAQVAVQRNDRLRIVSMPSDSE"
     gene            35962..36267
                     /gene="nuoK"
                     /locus_tag="PLH85_00190"
     CDS             35962..36267
                     /gene="nuoK"
                     /locus_tag="PLH85_00190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNB63004.1"
                     /translation="MLPLSHYLILGAILFAISVVGIFLNRKNLIVLLMAIELMLLAVN
                     INFVAFSRYLGDAAGQIFVFFVLTVAAAESGIGLAILVALFRNLRTIHVDDLDTLKG"
     gene            36280..38307
                     /gene="nuoL"
                     /locus_tag="PLH85_00195"
     CDS             36280..38307
                     /gene="nuoL"
                     /locus_tag="PLH85_00195"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNB63005.1"
                     /translation="MSDMQKLYLVVPLAPLLGAVIAGLFGKAIGRAGAHTVTILGVAI
                     SFVCSVFIFNDVAAGNTFNGTLYTWATSGALTFEVGFLIDTLTVMMMIVVTFVSLMVH
                     VYTIGYMTDDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWY
                     TRPTAIFANMKAFLVNRVGDFGFLLGIGLIAAYAGSLNYAEVFNKAGDLAGMTVPATG
                     WPLITAVCIGLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARM
                     SPLFELSDTALSFVLIIGATTALFMGFLGIVQNDIKRVVAYSTLSQLGYMTVALGVSA
                     YSAAVFHLMTHAFFKALLFLGAGSVIIGMHHDQDMRNMGGLWKYMPVTWITSLVGSLA
                     LIGFPFLSGFYSKDSIIEAVGVSHLAGSGYAYICVIVGVFVTAFYSFRMYFLVFHGKE
                     RFGLVHESHHDVHHHDDGDASHDEDHHHGLAPGQKPHESPWVVTVPLVMLAIPSMLVG
                     FYTIEPMLFGEYFKGVIYFSDAHPVMAELEEEFHGAVAMALHGFTTLPFFLAMSGVVL
                     AWFLYLVQPWIPEKVMLAFKPIHTLLENKYYFDKFNETVFAGGARLLGKGMWKAGDQA
                     IIDGMVVNGSAKFVGWIAQLTRLFQSGHIYQYAFSMIIGVFVLLVFWINRG"
     gene            38389..39870
                     /locus_tag="PLH85_00200"
     CDS             38389..39870
                     /locus_tag="PLH85_00200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNB63006.1"
                     /translation="MTGIPFLSLAIWVPIVGGLVALATGSDKNAPVARFVALFTALVG
                     LAVSIPLFTGFDVATSSMQFVEAYAWIPRFNINYYLGVDGISVLFVVLNSFITVIVVI
                     AGWDVIQEKVSQYMAGFLIMSGLINGIFTALDGVLFYVFFEASLIPLFIIIGVWGGPN
                     RVYAAIKFFLYTLAGSLLMLVALLYLFMESGGSFNILEWHELKLGMDVQTLIFWAFLV
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSIVLAAIGLKLGAYGFLRFSLPITPDAAQAFA
                     PMMITLSLIAVIYIGFVALVQTDMKKLVAYSSISHMGFVTLGFFIFNPLGVEGALVQM
                     ISHGFVSGAMFACIGVLYDRMHSRQIADYGGVVNVMPKFAALFMLFAMANSGLPATSG
                     FVGEFMVALGAVQHNFWVGFAAATTLILGAAYTLWMYKRVVFGAIANDHVAELKDINN
                     REFVFLLVLAVCVLAMGIYPFPVTNVMHASVSDLLKHVAAGKL"
     gene            39910..41376
                     /gene="nuoN"
                     /locus_tag="PLH85_00205"
     CDS             39910..41376
                     /gene="nuoN"
                     /locus_tag="PLH85_00205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNB63007.1"
                     /translation="MNFVTPDFYPAASELFVACMAIVVLMATTFAKDARSVGYWLSVL
                     TLVGAALVTLFTMDGQVSFTFSNMYVDDLMGDFLKLMLYFAVLVVLIYSRGYMNDRNI
                     ESPEFYLLVIFATLGMMVMVSANHFLTLYLGLELLSLALYAMVALQRDSVRCTEAAMK
                     YFVLGALASGLLLYGISMVYGATGTLEISGVAQAVYHQAGNRTVLMFGLVFLVAGLAF
                     KLGVVPFHMWIPDVYQGAPTSVTLLIASAPKLAAFAMAMRLLVNGLFDLADQWQSMLM
                     FMAVLSIVLGNLAAIAQTNLKRMLGYSGISHMGFMLLGLVSGVVGGDRHFALNAYSSS
                     MFYVVAYVLMSLASFGVLILMSRVGFEAETLDDFKGLNKRNSWFAGVMAISMFSMAGI
                     PFFIGFFAKFSVLQAVVAAGYIGVAILAVVMSLIGAYYYLRVVKVMFFDEPLDASVIH
                     APAEMRVMLSLNGIAIAVLGILPQGLMSVCAYALLGSL"
     gene            41431..41709
                     /locus_tag="PLH85_00210"
     CDS             41431..41709
                     /locus_tag="PLH85_00210"
                     /inference="COORDINATES: protein motif:HMM:NF022446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HNB63008.1"
                     /translation="MAVIIAAAVLANLSFISSRPFGVLPRRTLKSAWLHVSELGAAYL
                     VFLYFCLWMERAEHGVAYSQGWEFYAASLCVFLVFGFPGFVYRFFVSR"
     gene            complement(41837..43774)
                     /locus_tag="PLH85_00215"
     CDS             complement(41837..43774)
                     /locus_tag="PLH85_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB63009.1"
                     /translation="MMREALLRQPIKFKIIAIALITTAIGLLVALTAQVSSEFSATIE
                     TKQEHAVSLTTILGLNSTAALSFRDPEAGREILSALASNHEIIAARLRLPDGTIFARF
                     DSTRPEHADLIQSIPTAGQAELAHREHTLTALWHNPTLEVDQAVRLNDKVIGRIEVFF
                     DLSALMKLVRERILAAAGILLAATLIAWLLASRLQRVIAAPVEALAAQMQTVTETRDY
                     SVRVAPGGTDEIGTLMNGFNAMLEQIQTRDLQLSIAKDAAEQANKAKSLFLATMSHEI
                     RTPMNGVMGMAELLADSKLDAHQQRLLGHIQNSADSLLRVINDILDFSKLEAGRMELE
                     NIPFSPKSLIEEITAFFEPKAQEKGITLKPIFRNEIPPLVLADPNRLRQILMNLVGNA
                     MKFTQTGEIVVALEAHPMRHDAPEHRQLHFSVKDTGIGIPENVMPHLFSPFTQADSSH
                     ARRYGGTGLGLAVCRKLVKLAGGEIGVESSIRHGSKFWFTVPVKSTDSITVGLSQFER
                     QQPHLTATSNATKGLRVLIADDHRINQLLATMQLEELGCHVDTAGDGKDVLNLIARKD
                     FDIILMDCQMPELDGYETTRIIRARQRNNLRTPIIAVTANALDEDRATCLEAGMDDFL
                     PKPYTQNQLKEILLKWVPDNQ"
     gene            complement(43771..44337)
                     /locus_tag="PLH85_00220"
     CDS             complement(43771..44337)
                     /locus_tag="PLH85_00220"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB63010.1"
                     /translation="MHALSRFPGRWRLLLTLGVATLTAPASSVEPFAEAEVKAAFLYN
                     FVHFVRWPDVARKPGDPFRFCVLDDTLAPLLSKAVQGENVDNHSLTVTRQIELRNLAE
                     CQILYVGDAVWPVHPELLRRATALRILTVSDIPSFTEKGGMIALIRKRGRIHPVINMD
                     AIARADLRVSAKLLNLATVVGDDKGGAE"
     gene            complement(44389..46398)
                     /locus_tag="PLH85_00225"
     CDS             complement(44389..46398)
                     /locus_tag="PLH85_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB63011.1"
                     /translation="MPPLRPPHHLMAPLARTALALGIALLGTSAQADTDALSSLKQMS
                     LDELADIRVSIASQRSERVEDTAAAVTVLTAEDIRRSGATTIPELLRTVPGLNVARIS
                     ATEWAVSARGFNNQFANKLLVMVDGRSVYTPFFSGVLWDELNLVMQDIDRIEVVRGPG
                     GSTWGANAVNGVINVITRHSEDTQGTLVGTVVGNHQSEVVARHGYRLNDHSFGRVYAK
                     VHQADRFANPDPSTQDDMRWSGGRAGFRNDWESAGHTVTVQGEAFREMKPDERQYSGA
                     HLLGRWEHGHGDGSRDELRAYYNQFSLDHWRAAGNAATAQINTADVNFRHHFADAGPH
                     SLIAGFGYRAVQADANLTLPTSVTRPQRTDQLFSIFAEDTVSLAERLRLTLGVKGEHN
                     DFTGLEWQPSARLRWTSGSGSTAWMAVSRAVRTPSIIEDSATLNSSLAPTPQTFGLPV
                     AVSAVGNPNLASERLIAYEIGYRAQLSPRLSLDTAAFINDYDRLLSVDMIGTPSLVIP
                     PFPPRAMSWIARAGNNLEGRATGFEISLDYRPSTTWRLLTAYSYLDLNLHTKPGNTEL
                     LPLRAEGESPRHQISIISRSDLRHDLELDVQARYVSALPAQAVADYLTADMRLAWRAS
                     KSADLALVGRNLMGPAHTEFGGLALISSSAYPVPREYYLTANLRF"
     gene            complement(46583..49477)
                     /gene="gcvP"
                     /locus_tag="PLH85_00230"
     CDS             complement(46583..49477)
                     /gene="gcvP"
                     /locus_tag="PLH85_00230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNB63012.1"
                     /translation="MPQNLLSSPLSALEQRDAFIDRHLGPCAVERPPMLAALGVDSVE
                     TLIAQTVPAGIRLAAPLALGEPVPEHEALAELKAIASKNVLKKSLIGMGYYDTLTPKV
                     ILRNVMENPGWYTAYTPYQAEIAQGRLEALLNFQQMVIDLTGLELANASLLDEATAAA
                     EAMTMARRIAKVKSQAFFVDEAVFPQTLDVVRTRAEYFGFELIVGPAEDAPHHEVFGA
                     LLQYPNERGEVIDPSAAIAGLKAKGAVVAVASDLMALVMLKSPGALGADIALGSAQRF
                     GVPLGFGGPHAAFFATRQAHVRAMPGRIIGVSKDARGKTALRMSLQTREQHIRREKAN
                     SNICTSQVLLANMAGMYAVYHGPQGLRTIAARIHRLAAVLAEGLRSAAYTLRTDAFFD
                     TLQLLTGARTDALYAAAQAAGFNLRHLDGHGLGISVDEKTTAADVAALLGALGAPADI
                     AALDAAVAARGGNLPTGLLRDDAILSHPVFNTHHTEHEMLRYLKRLQNRDLALDHSMI
                     SLGSCTMKLNATSEMIPVTWPEFADIHPFAPAEQTAGYRELLDGLAAQLRAITGFDAI
                     CLQPNSGAQGEYAGLVAIRRYHAANGQGQRNVCLIPRSAHGTNPATAQMCGLEVVVVD
                     CDDNGNVDLPDLEAKAARFADRLAALMITYPSTHGVFEENIREICAAVHRHGGQVYMD
                     GANLNAQVGLTSPATIGADVSHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPYMADHAV
                     APTGDEARPQAGQGAVSAAPFGSASILPISWMYITMMGGRGLRQATEIAILNANYVAT
                     LLKDHYPVLYSGGQGRVAHECILDIRPIKAATGITEVDIAKRLMDYGFHAPTMSFPVA
                     GTIMVEPTESEDKGELDRFIAAMISIRAEIRAVEDGRLPADDNPLKHAPHTQADLLGD
                     WNRPYSRSEAAFPLPWVGENKFWPSVNRIDDVYGDRNLFCACVPMDAYAD"
     gene            complement(49540..49917)
                     /gene="gcvH"
                     /locus_tag="PLH85_00235"
     CDS             complement(49540..49917)
                     /gene="gcvH"
                     /locus_tag="PLH85_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNB63013.1"
                     /translation="MNTPDDLRYTASHEWVRTEADGTLTVGITDHAQEALGDVVFLEL
                     PDAGRSVEAGEAIAVIESVKAASDIYAPVAGEIIAANQDAVDAPESVNADAYAAWLFK
                     IKPAAGASLDSLLDAAGYAATLG"
     gene            complement(49969..51051)
                     /gene="gcvT"
                     /locus_tag="PLH85_00240"
     CDS             complement(49969..51051)
                     /gene="gcvT"
                     /locus_tag="PLH85_00240"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNB63014.1"
                     /translation="MPQHTPLYATHVAAGARMVDFAGWEMPVNYGSQIEEHHAVRRTA
                     GMFDVSHMLALDLDGRDARGFLRGLIANDVAKLSEPGKALYACMLNPDGGVIDDLIVY
                     HLSDTHYRVVVNAGTADKDVAWMHERLQASGADARLTVRRDLAMIAVQGPEARAKVWA
                     AIPGAEVVSAHLKPFQAVDFGEYFIARTGYTGEDGFEITLPAEKAAGLWVDLVNAGVA
                     PCGLGARDTLRLEAGMALYGNDMDENVSPLDAGLAWTVDLKDAARDFVGKAALIAQGT
                     RRQTLGLVLEDKGVLRSHQAVFCAQGDGETTSGTFSPTLQKSIALARLPLGVQPGDAV
                     EVDIRGKRLKARVVKPPFARNGQALI"
     regulatory      complement(51233..51324)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            51378..52253
                     /locus_tag="PLH85_00245"
     CDS             51378..52253
                     /locus_tag="PLH85_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB63015.1"
                     /translation="MWTSRSRFLPVRGLRYHLREWGREGAPRLFLLHGWMDVSASFQF
                     VVEALRHDWHVLAPDWRGFGHSEWRGDAYWFPDYLGDLDALLTALSPDAPVDLVGHSL
                     GGNVACLYAGVRPARVRRLVALDAFGLADTAPEQAPGRLEKWLRELSGPAAFRCYPDC
                     AALAERLRADNPRLSAERAAWLAEHLGEADGEGGIRLAGDPAHRRVNPVLYRRGEARA
                     CWQRVTAPTLWVVPEAAALRGRLGVSDEAHAEARDCFRDFREVTVADSGHNLHHDQPG
                     EVARIIETFLLSKAS"
     gene            52250..53089
                     /locus_tag="PLH85_00250"
     CDS             52250..53089
                     /locus_tag="PLH85_00250"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HNB63016.1"
                     /translation="MNPVNADLHCHSTASDGTLAPAQVVRRAHANGVDLLALTDHDEL
                     LGLAEAADAARDVGLRFVPGVEVSVSWLDQTVHVVGLNIDPSHPALVAGLHQVREGRD
                     GRAGQIAAELDRIGIHGALEGALRYAGNPALVSRAHFARYLVEIGIARNVHDVFLHYL
                     ARGKPGYVEHVWATLDEAVGWIRGAGGVAVIAHPGRYRLGHVEMDLLFERFRDLGGEG
                     VEVCCGAHDGGQVLAFSRVARKYGLLASRASDFHGPEDSPVDLGRAPPLPPDLTPVWT
                     RLQ"
     gene            53115..53738
                     /locus_tag="PLH85_00255"
     CDS             53115..53738
                     /locus_tag="PLH85_00255"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB63017.1"
                     /translation="MAQFFSLHPEHPQPRLIRQAAQIMREGGLVAFPTDSAYALGGHT
                     GDNELLSRIRRIRGVDERHHFTLICRDLSEIATYARVDNAQYRLLKATTPGPYTFILE
                     GTKELPRRVLHPKRKTIGLRVPDHPLVAALLAELDEPLLSSTLLLPGEEHPLTDAEEI
                     RDRLEKSVDLVIEAGPCRGEATTVIDLTSGTPELVREGRGSLAPFGL"
     gene            53815..54474
                     /locus_tag="PLH85_00260"
     CDS             53815..54474
                     /locus_tag="PLH85_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB63018.1"
                     /translation="MQELISSLAIWALPVLFAITLHEAAHGYAARHFGDRTAELAGRI
                     SLNPLRHIDPVGTLLIPGVILASSALVGGSPMLFGWAKPVPVNFGRLRHPKADMLWVA
                     AAGPLANLCMAIGWMVVLKLGFSLPENAYTLPLLKMADAGVQINAVLMLLNLLPLPPL
                     DGGRIVVSLLPAGPAWKFAQIEPYGFPILLLLLFTGILGDILDPLMALFRFALATVFG
                     L"
     gene            54481..55683
                     /locus_tag="PLH85_00265"
     CDS             54481..55683
                     /locus_tag="PLH85_00265"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNB63019.1"
                     /translation="MFAERVLSGMRPTGRLHLGHYHGVLKNWVKLQAEYPCLFFVADW
                     HALTTDYDNPKGIENSVWDMVVDWLAAGVDPSQATIFIQSRVPEHAELHLLMSMMTPL
                     GWLERVPTYKDQQEKLVNKDLSTYGFLGYPLLMSADILIYRADKVPVGEDQLPHVEFT
                     RELARRFNHMYGREPGFEDKAREAVKKLGSKRAKLYEEFRTRFQQNGDDEGLALGKAL
                     LDEAQNLSHGDRERLYGYLEGSGKMILVEPDALLTEASRMPGLDGQKMSKSYGNTIFL
                     REEADALTKKIRTMQTDPARVRRTDPGDPEKCPVWQFHVIYSDAPTREWVDRGCKSAG
                     IGCLECKQPVVDAVLKEQEAMRERAQPYLDDPTLVRSIIADGCDKARKLAQETMRDVR
                     ESMGLSYS"
     gene            55844..56614
                     /locus_tag="PLH85_00270"
     CDS             55844..56614
                     /locus_tag="PLH85_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNB63020.1"
                     /translation="MYGQPIFELPRDLYIPPEALEVFLDAFEGPLDLLLYLIRKANIN
                     VLDIPMVPLTAQYLEYVEAMRRHNLELAADYLLMAATLLEIKSRMLLPRPPRDDGEEV
                     LDPRAELVRRLLEYEQIKLAAARVDALPRAERDFEWVGVFVAEKVVERLPTVSLHDLQ
                     LAWLKIARQNRLRRHHTIQREELSVREHMSLILRKLSGDAFVVFESLFDPALGVAGLV
                     VVFLATLELVKERLVVVTQNEAFAPIYVKRSDASAADA"
     gene            56592..57167
                     /gene="scpB"
                     /locus_tag="PLH85_00275"
     CDS             56592..57167
                     /gene="scpB"
                     /locus_tag="PLH85_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNB63021.1"
                     /translation="MPPPQTPDEFKRVLETALLAAPAPLQVSELRRLFEDDPGGDLVR
                     RLLVELAGDWQGRGVELVQTASGWRFQTRPEYQIYLDRLKNERPPKYSRAVLETLAII
                     AYRQPVTRGDIEDIRGVMVSPSVLKTLESRGWVDVVGHRDTPGRPGLYATTRKFLEDL
                     GLRSLTELPPLTEIERIMDLVELPKTEEPAV"
     gene            57412..58272
                     /locus_tag="PLH85_00280"
     CDS             57412..58272
                     /locus_tag="PLH85_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB63022.1"
                     /translation="MPTSATPEREQRRPQGRDAAYTEPQRLHKVLAAAGLGSRREIEE
                     WIVAGRISVNGEPADVGQKVGPGDRVRVNGKLMPIRFQPRVPRVLLYHKPEGEIVSRD
                     DPEGRPTVFDRLPMLRKGRWIAVGRLDFNTSGLLLFVNDGDLANRLMHPRYELDREYA
                     VRLLGELDEVQKQALLDGIELDDGPARFNTLLDAGGEGVNHWYRVSLSEGRNREVRRM
                     FEALGLTVSRLMRVRYGPISLPTRLKRGMWEDMPEEDVCALAGLPKPIRAPGPRKPPR
                     STPRRTPPKG"
     gene            58431..58859
                     /gene="rimP"
                     /locus_tag="PLH85_00285"
     CDS             58431..58859
                     /gene="rimP"
                     /locus_tag="PLH85_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNB63023.1"
                     /translation="MDLQQLIEQTAEGLGYEVVDVESSPRGRLLRVFIDAPDGITVDD
                     CATVSNQLSRVFEVENVDYDRLEVSSPGLDRVLKKATDFERFAGETIQVRLNLPIDNQ
                     RNFTGTLVGLTDGVVTLETEKGSLAIPFEEINKARLVPRF"
     gene            58881..60353
                     /gene="nusA"
                     /locus_tag="PLH85_00290"
     CDS             58881..60353
                     /gene="nusA"
                     /locus_tag="PLH85_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNB63024.1"
                     /translation="MSREILLLVDALAREKNVSKEIVFSALESALAQATKKRIHDEAD
                     VRVSIDRETGDYESFRRWQVLPDEEVDNDEAQMGLIDAREEYPEIQVGDFVEESLEPI
                     DFGRIGAQAAKQVILQKVRDAEREQILNDFLERKEFIISGSIKRMERGNAIIEVGRLE
                     AVLPRDQMIPKENLRVGDRVKAYLLRIDRGARGPQLILSRTAPEFVIRLFELEVPEID
                     DGLLEIKACARDPGLRAKIGVKSNDPRIDPIGTCVGLRGSRVTAVRNEIGGENIDIVL
                     WSPEPAQFVIGALQPAEVSSIVVDEEAHAMDVVVDENNLAIAIGRNGQNVKLASELTG
                     WTINLMTEAESERKSEEEHSSVRQIFMSKLDVDEEVANILIDEGFSSLEEIAYVPLAE
                     MLEIESFDEETVNELRNRARNVLLTEAIVTEEQLETVSEDLLSLDGMDKPLAAKLAQA
                     GVHNRDDLADLAVDELMDMSGLDEERAKNLITRARAHWFE"
     gene            60369..63182
                     /gene="infB"
                     /locus_tag="PLH85_00295"
     CDS             60369..63182
                     /gene="infB"
                     /locus_tag="PLH85_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNB63025.1"
                     /translation="MGQTSVTQFAGELKMPASVLLEQLQKAGVDKSRETDLLTEQDKT
                     RLLDFLRRAHGDSAAKAKITLTRKQTSEIKATDSTGRARTVQVEVRKKRVFVKRDELL
                     VPEAAAPGAALVPEMPAELPEPVLEPTPVPEPIEMAAPEPVQAAPVEVVVEVEPEPVV
                     EPVAPEETVAPEPEPEPVAVVPEPVAVAPAAKPESRPQQGPVRRVVSSVISAEERASR
                     EREAQRQAELAARQAADLKEKQEREARARAAMLQRMEDEKQRQSEGDKSRQDAEAGAK
                     SGTLHKPAKADDKGARKDVKKGTPVAKEAAGDAKRRGGLKTRGDAAGGAGGWKGARGG
                     RSGGRHHEAPAFVAPTEPIIREVHVPETISVADLAHKMAVKATEVIKTLMKMGSMVTI
                     NQVLDQDTAMIVVEEMGHKALAAKLDDPDAFLEENAEHKDVVEESRAPVVTVMGHVDH
                     GKTSLLDYIRRAKVAAGEAGGITQHIGAYHVDTDRGTVTFLDTPGHEAFTAMRARGAK
                     ATDLVILVVAADDGVMPQTKEAIHHAKAAGVPLVVAVNKIDKPGANPERVRQELIAEG
                     VVPEAYGGDTMFIEVSAKTGQGIDDLLEAILLQAEVLELKAPHDTMAKGLIIEARLDK
                     GRGPVATLLVQSGTLKRGDVILAGATFGRVRAMLDENGKPVEEAGPSIPVEILGLSEV
                     PGAGDEVVALADERKAREIALFRQGKFRDVKLAKQQAAKLESMFEQMGEGEVKSLPLI
                     IKADVQGSQEALAHALVKLSNEEVRVQVIHGGVGAISESDVNLAQASGAVVIGFNIRA
                     DANARKLAENFGVDIRYYNIIYDAVDEVKSALSGMLAPEKREQVLGMVEIRQVFTISK
                     VGTIAGCYVLEGLVKRNAQVRVLRNHVVIHTGELESLKRFKDDVKEVKAGYECGLQIR
                     NFNDLVEGDQLEIYEIQEVARTL"
     gene            63186..63605
                     /gene="rbfA"
                     /locus_tag="PLH85_00300"
     CDS             63186..63605
                     /gene="rbfA"
                     /locus_tag="PLH85_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNB63026.1"
                     /translation="MPKEFSRGQRVAEQIRRELAELIRLEVKDPRVGFITLTDVELTP
                     DYAHAKVYFTSMTGQDGLDDILRGLRRASGFLRRELGRRVRIHTLPELHFQYDPSVEN
                     GNRLSSLIDRALESDRQFHPEDEAAGDDTSGKGPSVD"
     gene            63645..64565
                     /gene="truB"
                     /locus_tag="PLH85_00305"
     CDS             63645..64565
                     /gene="truB"
                     /locus_tag="PLH85_00305"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNB63027.1"
                     /translation="MVQAPPPRRVPRRIVDGVLFLDKPQGLSSNHALQQARRLLNAAK
                     GGHTGTLDPMATGLLPLTFGEATKFSQTLLDADKAYDATVLLGVTTTTADAEGEVLDT
                     RPVHVSDAQLAAALAQLRGEIEQVPPMYSALKHHGKALYEYAREGVEIPRAPRRVTIH
                     RLELLERAGDALRIRVDCSKGTYIRTLASDLGALLGCGAHLTALRRTRIGPFALDAAI
                     PLADFDACAPAARDAALAPVDALLVHMPCVSLDADQARSLTHGQAARGCAGEAGQAYR
                     VYHVGRFLGVATLAEGGLLVPTRLVSTADQ"
     gene            64639..64908
                     /gene="rpsO"
                     /locus_tag="PLH85_00310"
     CDS             64639..64908
                     /gene="rpsO"
                     /locus_tag="PLH85_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNB63028.1"
                     /translation="MAISTEQKAQIVRDHQRAQGDTGSPEVQVALLTARINDLTGHFK
                     AHVKDHHSRRGLLQMVSQRRKLLDYLKRKNADGYRELIARLGLRK"
     gene            65106..67205
                     /gene="pnp"
                     /locus_tag="PLH85_00315"
     CDS             65106..67205
                     /gene="pnp"
                     /locus_tag="PLH85_00315"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNB63029.1"
                     /translation="MPTSIKKTFTYGSHTVTFETGEIARQAHGAVMVTMDDTVVLATV
                     VAAKNAKPGQDFFPLTVDYVEKFYAAGRIPGGFFKREGRPTEKETLTSRLIDRPIRPL
                     FPDGFYNEVQVIVQAMSLNPEVDADIPAMLGASAALAIAGIPFAGPIGACRVGYANGE
                     YILNPTATQLKTSQMDLVVAGTETAVLMVESEARELPEDVMLGGVVFGHTQMQAAITA
                     INELVEVAGKPEWDWQPPAKDEALIARVTEIAGEKIDAAFRITAKQARSQQLSAIGDE
                     VHAALTAEGRAYDANTVNNILFDLESKVVRGRILSGEARIDGRDTRTVRPIDIRVGVL
                     PRTHGSALFTRGETQALVVTTLGTGRDEQIIDAVAGEYRERFLLHYNFPPFSTGECGR
                     FGSPKRREIGHGRLAKRALAAVLPPKEEFTYTVRVVSEITESNGSSSMASVCGGSLSM
                     MDAGVPLSAPVAGIAMGLIKDGGRFAVLTDILGDEDHLGDMDFKVAGTDKGVTALQMD
                     IKILGITKEIMQVALAQAKEGRLHILGLMKDALGEARGEVSTFAPRMLTIKINPEKIR
                     DVIGKGGAVIRALQEETGTIIEITDDGNVTISSVSAEGAQEAKKRIEDITAEVEVGKV
                     YEGPVVRILDFGAIINILPGRDGLLHVSQIANERVNAVSDYLKEGQVVRVKVLETDEK
                     GRVRLSMKALLNEQAAQ"
     gene            67348..68808
                     /gene="gabD"
                     /locus_tag="PLH85_00320"
     CDS             67348..68808
                     /gene="gabD"
                     /locus_tag="PLH85_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNB63030.1"
                     /translation="MKSLKDPLLFRTQAFINGVWTDADSGARLEVCNPFDGNSLGSVP
                     AMGATETAGAIAAANAALPAWRSLLARERAAILQRWFQLIHTHADDLARMMTLEQGKP
                     LAEARGEVVYAASFVEWFAEEAKRLYGELIPTTGADRRLMVIRQPVGVCAAITPWNFP
                     AAMITRKVAPALAAGCTVIVKPAEQTPFTALALAELAHRAGFPRGVFNVLTGDPVAIG
                     GALTASEVVRKLSFTGSTEVGRLLMAQCAPTVKKLSLELGGNAPFIVFDDADLDAAVA
                     GALVSKYRNAGQTCVCANRFLVQDGIYDAFAACLAEAVKGFSVGNGLDAGVTIGPLID
                     AAAVDKVEAHVADALAQGARVLVGGRRHARGGTFYEPTVLADVTPAMRVAREETFGPV
                     APLFRFKTEAEAIHMANDTEFGLAAYFYARDVGRIFRVGEALEYGMVGINTGLISNEV
                     APFGGMKQSGVGREGSRHGIDEYLELKYLCLGGLDR"
     gene            68805..69353
                     /locus_tag="PLH85_00325"
     CDS             68805..69353
                     /locus_tag="PLH85_00325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB63031.1"
                     /translation="MMRHAASLTPSAWVTRFAPLIAAGGEVLDLACGSGRHARWLAGR
                     GYRVEAVDRDAVALELLATVPHVTTREADLEAGDWPYGDRRFDGVVVTNYLFRPRFNL
                     LLAALNPGGVLLYETFMLGNERFGKPSNPEFLLRSHELIERVAGACTILAYEQGEVSE
                     PKPAVIQRICAVKGHNPVVRLP"
     gene            complement(69372..69992)
                     /locus_tag="PLH85_00330"
     CDS             complement(69372..69992)
                     /locus_tag="PLH85_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNB63032.1"
                     /translation="MRAAALLRRAALAVLLLTAGAPAFAGKILVWGDSLSAGYGLRPQ
                     EAWPDLLGQRLAGEKSAWTVVNGSISGETTAGGRARLPAALDRQRPAVVIIELGANDG
                     LRGLPVPAMVANLQAMIDAARKAGAQVLLVGMRMPPNFGPDFTARYEKAFRDLARTNT
                     LRFVPFLMDGFADQPAFFQPDGLHPVASAQPRILETVWKELKPLLH"
     gene            complement(70000..70806)
                     /locus_tag="PLH85_00335"
     CDS             complement(70000..70806)
                     /locus_tag="PLH85_00335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNB63033.1"
                     /translation="MHPTLNIAIKAARRAATVINRASLDVDLLRIETKQPNDFVTEVD
                     RAAESAIIDVLREAYPSHAILAEESGETGPAEDTEYQWIIDPLDGTTNFIHGFPQYAV
                     SIALARRGVVEQAVVFDPTRNELFTATKGGGAFLNDRRIRVSKRLRLQDALVGTGFPY
                     RQFAHVDTYLAIFKELTQKSAGLRRPGAAALDLAYVAAGRLDAFWEFGLAPWDMAAGS
                     LLISEAGGLVSDLAGESTYLTTGNVVAGTPKVFPQLLQLIQGHCEGQLTA"
     gene            70885..71607
                     /locus_tag="PLH85_00340"
     CDS             70885..71607
                     /locus_tag="PLH85_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB63034.1"
                     /translation="MNREGALGRVRIVLSRTSHPGNIGAAARAMKTMGLSQLVLVRPQ
                     SFPDPVATARAAGADDILAAARVVDTLEEALQGTVFSAALTARRRELAVPMRWAREAA
                     GELVCAAHGGDVALVFGNETVGLSNEEVSLCQMPVRIPTAAEYGSLNLGAAVQVLAYE
                     LRMAALEPGEPPGTAFEPATHEEIEGFYAHLDRAITASGFFNPVNSKRLWPRLRRLYG
                     RIRLERDEVNILRGMLTAFEKK"
     gene            71688..72452
                     /gene="cysE"
                     /locus_tag="PLH85_00345"
     CDS             71688..72452
                     /gene="cysE"
                     /locus_tag="PLH85_00345"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNB63035.1"
                     /translation="MFRRLREDVANVLERDPAARSRWEVLTCYPGMHAVLLHRVAHAA
                     WGRGLHWVGRFVSHLGRMLTGIEIHPGATIGRRVFIDHGMGVVIGETAEVGDDCTIYQ
                     GVTLGGTSLYRGTKRHPTLGRGVVVGAGAKVLGGFEVGDGAKIGSNAVVIKPVPAGAT
                     AVGNPARIVDAGKDESPDVKADAPARQAFTAYGVTRDLDDPLVKALHGMIDHAAETDR
                     RLRVLVERLAKAGIPVDDAVLREDGFDPQKLGKMVD"
     gene            72557..73042
                     /gene="iscR"
                     /locus_tag="PLH85_00350"
     CDS             72557..73042
                     /gene="iscR"
                     /locus_tag="PLH85_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNB63036.1"
                     /translation="MRLTTKGRFAVTAMIDLALRCADGPVTLAGIAERQRISLSYLEQ
                     LFGKLRRFELVSSVRGPGGGYTLAQPMELVTVADIIAAVDEPLDATSCGGKENCLDEH
                     RCMTHDLWTNLNRRMQEYLQSVTLASLVDAQRQKALAVSVLHDERAAIARHEAGQSLT
                     A"
     gene            73059..74225
                     /locus_tag="PLH85_00355"
     CDS             73059..74225
                     /locus_tag="PLH85_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNB63037.1"
                     /translation="MFAPIYLDHNATTPLDPEVREAMLPWLGGRFGNASSRHEYGRAA
                     RMAIDEARARVAEAVNAHPTEVIFTSGGSEANNLFLKGAAARLKAGALALGAIEHPCV
                     RQPARQLRQSGWTVRELAVDAAGRIDSAAFTAVLAERPRLVSVMLANNETGVVQDVAR
                     LAAQVRAAGGWMHTDAVQAFGKLPVDFRALGVHGMSLSAHKVNGPVGAGALIVDKRVE
                     LAPLVAGGGQERDLRSGTENVLAIVGFGKACQLAAARQADRAAVLRGLQARLDAGLTG
                     LGATIFSAGAERLPNTTFFALPGIDGETLVGKLDRAGFAVASGSACSSAHPEPSPTLL
                     AMGVTPECARAAVRVSLGSGNTGQDVDGFLEAIAQIVDHLRNLTAMAVGVADAA"
     gene            74250..75461
                     /locus_tag="PLH85_00360"
     CDS             74250..75461
                     /locus_tag="PLH85_00360"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNB63038.1"
                     /translation="MLKFPIYLDYSATTPVDPRVAEKMIPYLTELFGNPASRSHAFGW
                     SAEKAVEEAREQVAALVNADPKEIIWTSGATESNNLAIKGAAHFYSGKGKHLITVKTE
                     HKAVLDTVRELERQGFEATYLDVQDNGLIDLEVLKAAIRPDTVLVSVMFVNNEVGVVQ
                     PIAEIGELCREKGIVFHVDAAQATGKVDIDLARLKVDLMSFSAHKTYGPKGIGALYVR
                     RKPRVRLEAQMHGGGHERGLRSGTLATHQIVGMGEAFRIAREEMAQENARILALRNRL
                     FEGLSAIPETFVNGDLVHRVPHNLNMSFAYVEGESLIMAIKDVAVSSGSACTSASLEP
                     SYVLRALGRNDELAHSSIRFTIGRFTTVEEIDYTIDLLQRKIGKLRELSPLWEMVQEG
                     IDLNTVQWAAH"
     gene            75475..75858
                     /gene="iscU"
                     /locus_tag="PLH85_00365"
     CDS             75475..75858
                     /gene="iscU"
                     /locus_tag="PLH85_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNB63039.1"
                     /translation="MSYSEKVLDHYENPRNVGSFQKEDDGVGTGMVGAPACGDVMKLQ
                     IKVGKDGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDQALEIKNTAIAEELALPPV
                     KIHCSILAEDAIKAAVEDYKKKHNG"
     gene            75872..76195
                     /gene="iscA"
                     /locus_tag="PLH85_00370"
     CDS             75872..76195
                     /gene="iscA"
                     /locus_tag="PLH85_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNB63040.1"
                     /translation="MAVTLSEKAAQHIDNFIAKRGKGIGIRLGVRTSGCSGMAYKLEF
                     ADEVQSDDVVFESRGLKVLVDPKSLPYMDGTELDFVREGLNEGFKFNNPNVKDQCGCG
                     ESFTV"
     gene            76197..76727
                     /gene="hscB"
                     /locus_tag="PLH85_00375"
     CDS             76197..76727
                     /gene="hscB"
                     /locus_tag="PLH85_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNB63041.1"
                     /translation="MIDLRQDHFALFGLAPAFHIDLDQLEQAYLTLQGKVHPDRFAHL
                     SDQEKRQSMQWATHANGAFRTLRQPLTRAQYLLELRGVDPAFENNTAMAPEFLMEQME
                     WREALGEAREAADASALDDMARRIRERSRALVVQIGDEIDTLGDLPAAADTVRRLKFL
                     ERLQDEINDALASLDS"
     gene            76727..78595
                     /gene="hscA"
                     /locus_tag="PLH85_00380"
     CDS             76727..78595
                     /gene="hscA"
                     /locus_tag="PLH85_00380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNB63042.1"
                     /translation="MALLQISEPGLSTLPHQHRLAVGIDLGTTNSLVATVRNGVPQCL
                     HDDDGRVLLPSVVQYLPDGRVAVGRGAQKAQAVDPRNTIVSVKRFMGRGLKDVAHVET
                     MPYDFVDQQGMVRLRTVQGVKSPVEVSAEILSSLRLRAERSLGGELTGAVITVPAYFD
                     DAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLENASEGVYAVYDLGGGTFDISILR
                     LSRGVFEVVATNGDAVLGGDDFDHRLFCWILDNAGISLPSDQDARRLQMKAREVKERL
                     TTDESVDIRLTLTRGDEVDLTLTREQFVEMTRHLVSKTLAPVRKALRDAGLAPEDVKG
                     VVMVGGATRMPHVQKAVGEYFGQTPLTNLDPDTVVAIGAAIQANVLAGNRAAEDDWLL
                     LDVIPLSLGIETMGGLVEKIIPRNATIPTARAQDFTTFKDGQTAMALHVVQGERELVS
                     ECRSLARFELRGIPPMVAGAARIRVTFQVDADGLLSVSARETLSGVEARVEVKPSYGL
                     SDDEITRMLQSGFEYARADMEARGLKEQQVEAARVIEATEKALMSDGDLLTADERTAL
                     DASVEALRSAAAGLDARAIKLALESFNRASNEFASRRMDRSIRAALSGHKIDDIRI"
     gene            78619..78963
                     /gene="fdx"
                     /locus_tag="PLH85_00385"
     CDS             78619..78963
                     /gene="fdx"
                     /locus_tag="PLH85_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNB63043.1"
                     /translation="MTTLIVLPHVELCPEGAVIDARPGESICQSLLENGIAIEHACEQ
                     SCACTTCHVIVRDGFNTLEPAEDEEEDLLDKAWGLEPQSRLSCQALVGGTPLTVEIPR
                     YSINMVKEGKRG"
     gene            78960..79154
                     /gene="iscX"
                     /locus_tag="PLH85_00390"
     CDS             78960..79154
                     /gene="iscX"
                     /locus_tag="PLH85_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNB63044.1"
                     /translation="MKWTDVQEIAIQLSEARPDADPARVNFVDLMSWVMALPEFDDDP
                     KRCGERILEAIQQAWIDEVA"
     gene            79177..79665
                     /locus_tag="PLH85_00395"
     CDS             79177..79665
                     /locus_tag="PLH85_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNB63045.1"
                     /translation="MSGGAQLHAFELPALQGGVLRLADYAGKVLLIVNTASRCGFTPQ
                     YAGLQALYETYGAQGLVVIACPCDQFGGQELGSAEEIGAFCTGRFDVRFPMADKLRVN
                     GPDAHPLFHYLTAALPGVLGSRAIKWNFTKFLVDRVGNPVRRYAPTTPPAELAGDIEA
                     LL"
     gene            complement(79670..80119)
                     /locus_tag="PLH85_00400"
     CDS             complement(79670..80119)
                     /locus_tag="PLH85_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB63046.1"
                     /translation="MATDRPILLVEDNPDDEALTLRAFSKNKIANQVVVARDGVEALD
                     YLFCTGQYSTRDSHQMPAFVLLDLKLPRIDGLEVLRRIRADERTALLPVVVLTTSKEH
                     QDIFEAYRLGANSYIRKPVDFERFLQAVGQLGLYWLSLNEPSDPTAR"
     gene            complement(80259..81209)
                     /gene="prmB"
                     /locus_tag="PLH85_00405"
     CDS             complement(80259..81209)
                     /gene="prmB"
                     /locus_tag="PLH85_00405"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNB63047.1"
                     /translation="MAHEDTLPDDAGHDEHEHEHEHGPLAELVTVRDWLRYAVTRFNR
                     AGCFFGHGFVDAWDEAVYLVLHTLALPLDRLDPFLDACIPGDEREAIFEVIEKRAVDR
                     LPAAYITGEAWLGEFRFEIDPRVIIPRSYFAELLHEGFSPWIQDPASVTRAMDMCTGS
                     GCLAILMAHAFPHAEIVAVDISPDALEIAARNVAAYGLEDRITLVQSDAFDQVPEQPF
                     DFILSNPPYVTQDAMDDLPAEYRHEPALALGAGEDGLDLVRRLLADAPRFLAPDGQLA
                     VEVGHNRDLFEAAFPTLAPTWLSAPSGDEKIFLINRRQLL"
     gene            complement(81221..82369)
                     /gene="dapE"
                     /locus_tag="PLH85_00410"
     CDS             complement(81221..82369)
                     /gene="dapE"
                     /locus_tag="PLH85_00410"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNB63048.1"
                     /translation="MTRPTAPASATLALTRELIARRSVTPEDAGCLDLIASRLTPLGF
                     VCERLDAGGTCNLWARRGTARPLVVFAGHTDVVPTGPLAQWQSDPFEPTERDGFLYGR
                     GAADMKTSLAAFVTAIEDFVASHPHHPGSIALLLTSDEEGDATYGTVHVVNTLKARGE
                     TLDFCIVGEPTSVDVLGDMIKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPAL
                     TELAATRWDEGNEYFPPTSWQMSNIHAGTGTTNVIPGTLEVLFNFRFATVHTADALKQ
                     RVHAILDRHGLDYDLAWTLGGKPFLTPRGTLVGALEAAIRDALGRTPELSTTGGTSDG
                     RFIADICPQVVEFGPVNATIHKIDECIALDAVDPLSDIYRRTLEHLLA"
     gene            complement(82391..83566)
                     /locus_tag="PLH85_00415"
     CDS             complement(82391..83566)
                     /locus_tag="PLH85_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNB63049.1"
                     /translation="MIIDKLFTLMAEKKASDIYVTVGAPIHIKIDGNTLPINQQAMDA
                     GMIHRMAYEMMTPEQIARFENEREMNLSFSRRNMGNFRVNIFWQRNSIAIVVRYILDQ
                     IPRLDALGLPPVLSDVIMEKRGLVLVVGATGSGKSTTIASMLDHRNENFSGHILTIED
                     PIEYLFRHKASIVNQREIGTDTLDWNHALSNAMRQAPDCILIGEIRDRDTMQAAIAYA
                     QTGHLCLATLHANNAYHALNRIFNFFPMENRPLLYMDLAATLKCIISQRLVKRPDGKR
                     IPAVEILLNSRQVAELIERGEISGIREAMEQSLASGSRTFEQDLFRLYRDKIITLEEA
                     LANADSPTNLSWLINNAQFSDKNADKTPLPSTSIDSEPHQEEGGASFKEFTLELDED"
     gene            complement(83620..>84039)
                     /locus_tag="PLH85_00420"
     CDS             complement(83620..>84039)
                     /locus_tag="PLH85_00420"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNB63050.1"
                     /translation="VDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTI
                     IGDNCFIGARSEVVEGVIVEDNCVISMGVYIGQSTKIYDRTTGEVLYGRVPEGSVVVS
                     GNLPSGDGKYSLYCAVIVKKVDAQTRSKTSINELLRD"
     assembly_gap    84040..84139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<84140..85335)
                     /gene="dapC"
                     /locus_tag="PLH85_00425"
     CDS             complement(<84140..85335)
                     /gene="dapC"
                     /locus_tag="PLH85_00425"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNB63051.1"
                     /translation="MNPHLQDLHPYPFEKLRALFDGIVPPAEYSPIRLSIGEPQHATP
                     AFIRDALVAHLGGLANYPTTQGSDALRKSIAAWLESRYGLPAVDPATQVLPVNGSREA
                     LFAFAQCVIDGTRPDALVLCPNPFYQIYEGAAYLAGAQPYFINNLPDDNFGSDFARVP
                     DEAWARVQLVYVCSPGNPTGRVLTLDEWQHLFELSDRHGFVIAADECYSEIYFNDDAL
                     PIGGLEAAQRLGRSDFRNVVMFSSLSKRSNVPGMRSGFVAGDATILKRFLLYRTYHGC
                     AMSPVVQAASAAAWSDEAHVAENRRLYREKFDRVTPLIAEHLEVSLPDAGFYLWARVD
                     KLGLSDTEFARRLQAEYNVTVLPGSFLAREAHGTNPGAGFVRIALVAELAECLEAARR
                     IAAFAQR"
     gene            85539..89060
                     /gene="smc"
                     /locus_tag="PLH85_00430"
     CDS             85539..89060
                     /gene="smc"
                     /locus_tag="PLH85_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNB63052.1"
                     /translation="MRLAKLKLAGFKTFVDPTTVLTPGNLVGVVGPNGCGKSNIIDAV
                     RWVLGESRASALRGGSMLDVIFNGSTTRKPVSRASVELVFDNAEGRAAGQWKPYAEVA
                     VKRVLDRSGESTYFINGLQVRRKDVIDLFLGTGLGPRAYAIIEQGMISRIIEARPEEV
                     RGFLEEAAGVTKYRERRKETEGRLTDARDNLARLEDIRHELGQQIGRLEAQAAVAERY
                     HRLNASLSERQNLLWLIKRNEARSERTRLTEELGEATRRIEADSARLQTLESQLESAR
                     EAQFAASGHVQHAQDALHGVSTELTRLEGELARLRDSRRVLESRLVQLERDDAQWRSQ
                     LDTLSSDAERWHSLQEHAALRAEQAEARHAELAERLPEAEDALRTADATAAAVRRELN
                     QAEQQIRVEEAHRVSSERTLETLGQRRGRLQQEQSAVDAPDLRRIAEREALLSELEDT
                     LATRQSAVADAQAGLPGAQQALREAMEAERQTQRQATELRARREALVQLQHKVQAQGQ
                     LGDWLRRHRLDGLTPLWQRLQVAAGWESAVEAILRERLAALPAEDGRAALIALADKVP
                     GAFAFLLADAPADGAPPMALEGARPLLDHVCADVTLTPVLAAWLGTVLAVDDLAPWLE
                     RRAELSPSVRLVAKDGRVLDRHALIHYAPDARTHGVIERQREIDQLAVELDRAQEAAA
                     QAKEAVAEADATATRLQDGLTTLRRDVQALQAQVHGEQVEVLKLNQARVRYEERWAQI
                     ERDMGEIALLEQTERDRLMRSETELERSREMAELQRGRLDAAEAVRREREAALRETRA
                     LEQALARELQDARHAERECTSKLDDIARNRSLAEEQLARTAEELEARRESLLEIDEPS
                     VAAALQAAVVLRGTREMALVEVRNGLEAIHAQVRGLEEARHHTEQTAAPVRERVAELR
                     LRVQAAELAEAQFATRLEEAQADEAALAPLLTADLKEGALQREVARLGREIAELGAVN
                     LAALDELRAASERKTYLDVQSEDLLGAIETLEDAIRRIDRETREQLQETYNTVNRHFG
                     SLFPQLFGGGEARLVLTGDEILDAGIQIVAQPPGKKNSSIQLLSGGEKALTAIALVFA
                     MFQLNPAPFCMLDEVDAPLDDANTGRYCGMVKRMSSQTQFIFISHSKITMEIAQQLVG
                     VTMQEQGVSRVVEVDIEEAMRLAETAA"
     gene            89088..90164
                     /locus_tag="PLH85_00435"
     CDS             89088..90164
                     /locus_tag="PLH85_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNB63053.1"
                     /translation="MSELQMGLIGAGGAVVLGIFAFNKWQERRQRKLAEPPVPTEPQA
                     SGTAARGGEARIEPVLGAATPRPVAARPAPTAGPRRVPPPLPAEVDERADCVVRVESI
                     EPVQGSRLLQAAAQHLQGIAKPVRWFGFSDHDNRWETLDGAPGHGYHWICAALQLVDR
                     RGPVSEGDLSAFCGGLQRMCDIFMGIPSLPVKAEALDRAAELDRLCAGVDIQIGVNVV
                     ARELPIPGTKLRGLAEAAGLELGTDGCFHARDDLGLSQFVLSNLEPVRFVAEEMRGLS
                     TQGVTLTLDVPRVADGVKVFDRMMTVAQQLAEALNGAVVDDNRTPFGDKAAGLIRAQI
                     EQFQVQMGEQGIAPGSTLARRLFA"
     gene            90174..92291
                     /gene="ligA"
                     /locus_tag="PLH85_00440"
     CDS             90174..92291
                     /gene="ligA"
                     /locus_tag="PLH85_00440"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNB63054.1"
                     /translation="MSVPAEQIARAAALREALERYNHAYYVLDEPLVPDAEFDARFRE
                     LQALEATYPELLTPDSPTQRVGGQPAPELAPVSHAVPMLSIRTETDTTAEGAVVFDTR
                     VRRALGLTDDAPPVTYAAELKFDGLAINLRYEGGTLVLAATRGDGTTGEDVTRNVRTI
                     RQIPLRLKGALPPVLEVRGEIYMRRDAFDRLNADQAAAGQKTFVNPRNAAAGSLRQLD
                     PAITARRPLSFFAYGLGEVVGWELPPTHAELLDALDALGFPVCVHRTRAQGAAALAQF
                     HEAMGVLRDGLPFDIDGVVYKVDSLALQQALGFVSREPRWAVAHKYPAQEAVTYLLGI
                     DVQVGRTGALTPVARLEPVFVGGVTVTNATLHNQGEIDRKELRVGDRVVVRRAGDVIP
                     EVVGRVIGDEEEEAAHVARPRFDLLGTYPVCPECGSHVVRGEDEAVARCTGGLYCPAQ
                     RKQALLHFAGRRAIDIDGLGEQIVHQLVTNSTVTTPADLYNPDRINANVLAGLERMGE
                     KSAANLMAAIEASRETSLARFIFALGIRNVGEKTAKDLARHFGGLKALMAADEAQLLQ
                     VPDVGPVVARCIVEFMGEARNQAVIGSLADALHWTEGAPAARSADGGLAGKTFVLTGT
                     LPTLKRDAAAALIEAAGGKVSGSVSKKTDYLVAGAEAGSKLDKAQQLGVMILDEAGLM
                     ALLEPVPPAQDGGGQGSLFEDQE"
     gene            92288..93175
                     /gene="galU"
                     /locus_tag="PLH85_00445"
     CDS             92288..93175
                     /gene="galU"
                     /locus_tag="PLH85_00445"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNB63055.1"
                     /translation="MKKVTKAVFPVAGMGSRFLPATKASPKEMMPVVDKPLIQYAVEE
                     AVAAGMTDLVFITGRSKRAIEDHFDKAYELETELEARGKTELLKIVRETVPANVNCIY
                     IRQPEALGLGHAVLCAAPVVGDEPFAVLLADDLLDGQGASSVMRQMADVYHYHRCSVL
                     GVMNVPREHTRQYGIVSTERQDGDVQRVTGIVEKPKPEEAPSTQAVVGRYVLTGRIFE
                     HLRKLTPGAGGELQLTDAIASLLREEAVLSYQFKGVRYDCGSKLGYLQATVELGLKHP
                     EVGAEFAAYLASRAGVDGA"
     gene            complement(93266..94684)
                     /locus_tag="PLH85_00450"
     CDS             complement(93266..94684)
                     /locus_tag="PLH85_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HNB63056.1"
                     /translation="MKSLFARLAAFIQPQYARVYDRSSRAFDRLLDGAERGEIHDDTD
                     YLADAEWAMAEQRLRGSRIAVWITLVAVLVLLLWAAFAQLDEVTRGEGKVIPSKQVQI
                     LQSLDGGIVSEILVKEGQQVKAGELLLKVDPTRFVSSLRENRAQYLALLAKAARLTAI
                     SHGKPFVAPPEVLKDAPDLVEQERSAYESKRAEMDAAASIARQQLSQRRQELAEVSAR
                     RDQSSQSYDLTARELEVTRPLAKTGAVSEVELLRLERDVARFKGERNAAVAQIPRIQA
                     AIAEAQNKVEEVELQIRNQARSELSDTNAKIAALSAGSEGLADRVKQAEIRAPMNGTV
                     KQLFANTVGGVVQPGKEIIEIVPSDDTLLLEARVQPKDIAFLRPGQPALVKFTAYDFA
                     IYGGLDATLEQIGADTITDEKGNAYYVVRVRTVHSALGDARLPIIPGMVAEVDILTGK
                     KSLLSYLLKPVLRAKANALTER"
     gene            complement(94689..96851)
                     /locus_tag="PLH85_00455"
     CDS             complement(94689..96851)
                     /locus_tag="PLH85_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HNB63057.1"
                     /translation="MTNPTSRSGWQIGEHATHDDPLLDALVLLTRIHGNPFTPEALSA
                     GLPLVDHRLTPSLLPRAAARAQLTARIVRKPLAEITPNFLPALLLLHDRRACLLLERL
                     PDGACRVRFPEAGESVDVLSADELAARYTGLVCFVRPRFRFEARAPEIRSLRGQHWFW
                     GTVLDNWRLYRDTLLAALVINLFALAIPLFSMTVYDRVVPNRAVETLWVLAVGTALLL
                     CFDFALRTLRAYIVDTAGKRIDVQLSARIMERVLGLRMEARPASVGSFAANLRAFEGV
                     RDFIASATVTTLIDLPFVLLFLLAMAWISPWLMIPPVVGILVVLFVTLIAQDKMHALT
                     ETTYRAGAQRNATLVESLVGLETLKTLAAEGLIQRQYERATLFIAQIGGRLKLLSAGT
                     VNFAQFMQQFVSVAVIIVGVYELADAKMSLGGIIAASMLAGRTMAPLGQVAGLLMQYH
                     NARTALETVEGHMQLPVERPDGANFVHRPAFRGEIEFKDVSFAYPGRDQAALHTISFR
                     LQAGEKVAIIGRIGSGKTTIEKLILGLYQPTAGAVLIDGIDARQVDPAELRRAIGFVP
                     QDVTLFYGSLKQNIAMGAPFADDAAVLAAAELAGVKEFADAHPQGFDMPIGERGESLS
                     GGQRQAVAIARAVLNDPPILLLDEPSSSMDHQSEEQLKQRLRAFAAGKTIVLVTHRTS
                     LLDLVDRLIVLDQGHIVADGPKAQVVEALQQGRIGRGG"
     gene            complement(97004..97336)
                     /locus_tag="PLH85_00460"
     CDS             complement(97004..97336)
                     /locus_tag="PLH85_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63058.1"
                     /translation="MSFVQRNAEGCIVALFADAGPGHSEELPADHPEVAAFLGGRGAA
                     DGAVTFTALDADLIRVIEDVVDVLIDRGLLRLTDLPPDAQRKLLARKGARERLRNGLN
                     LLGKHDVI"
     gene            complement(97422..98354)
                     /gene="ispH"
                     /locus_tag="PLH85_00465"
                     /gene_synonym="lytB"
     CDS             complement(97422..98354)
                     /gene="ispH"
                     /locus_tag="PLH85_00465"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNB63059.1"
                     /translation="MEVLLATPRGFCAGVERAIEIVERALARFGAPIYVRHEVVHNRF
                     VVEGLRAKGAIFIEDLADVPAGNTVIFSAHGVPQSVRSEAEARGLRVFDATCPLVTKV
                     HLEVARMRDQGREIVMIGHKGHPEVEGTMGQVKDGIHLVESVGDVATLQVNTPDALAY
                     VTQTTLSVDDAAAIVGALRERYPAIVGPKKDDICYATQNRQDAVKFMAPQSDLVLVVG
                     SPNSSNSNRLREVAELLQVPAHLVDNADAIDPAWLTGKQRIGVTAGASAPEVLVESVI
                     ARLKALGAASVRQLEGVPEKVTFPLPRELQTPAA"
     gene            complement(98362..98793)
                     /locus_tag="PLH85_00470"
     CDS             complement(98362..98793)
                     /locus_tag="PLH85_00470"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB63060.1"
                     /translation="MTQTVQPDSLVTLHYRISLDNGQPLISTFEAQPATMHLGQGDIA
                     PTLERCLAGLAVGERHSFELAPENAFGAHRAELVEKVRLEDFPDGAEVEPMVIMEFTA
                     PDGSRYPGLIREVLDTHAVIDFNHPLAGKPIRFEVQIIGIN"
     gene            complement(98790..99284)
                     /gene="lspA"
                     /locus_tag="PLH85_00475"
     CDS             complement(98790..99284)
                     /gene="lspA"
                     /locus_tag="PLH85_00475"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNB63061.1"
                     /translation="MKLRLGNWIGLAAVVVLLDQWTKHLVRGALRTGEVIPVTDFFDL
                     VLAFNPGAAFSFLADQAGWQRWFFVALAAVICGWLLRLVARHQHEFLQPLAFSLIIGG
                     AIGNVIDRFVFGAVVDFLYFHVGRYGWPAFNVADSAICVGVTLMVIAQLREHRHAPTR
                     ETLS"
     gene            complement(99306..>100912)
                     /locus_tag="PLH85_00480"
     CDS             complement(99306..>100912)
                     /locus_tag="PLH85_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB63062.1"
                     /translation="HSYMHCWRHKTPLIYRATAQWFVGMDLKPEGGPSLRELALQGVE
                     ATRFFPAWGQARLHAMIANRPDWCISRQRNWGVPIPFFLHKATGELHPRTVELMEAVA
                     TRVEQEGIEAWFKLDPAELLGDEAAQYDKISDTLDVWFDSGTTHWHVLRGSHPDGHAT
                     GPRADLYLEGSDQHRGWFHSSLLTGCAIDGHPPYHALLTHGFAVDGSGRKMSKSLGNV
                     VVPQEVTGKLGAEILRLWVASTDYSGELSISKEILDRVVEVYRRLRNTLRFLLANTAD
                     FNPATDMLPVDQWLEIDRYALALTRRLQQQAEADYARFEFHRVVQALQNFAAEDLGAV
                     YLDILKDRLYTTAATSAARRSAQSALWHILQAVVKLMAPILSFTAEEIWLLLGKDADD
                     SVMLHTFHTLPTQDGEDALLARWEAIRAVRAEVMKQIEAVRSDGKLGSSLQAEVELRA
                     AGAQYDLLASLGDDLRFVLICSKTVLTRVDSEADAAIVVSPSAQRKCERCWHYRDDVG
                     HDPAHPELCGRCTSNLHGTGETRAHA"
BASE COUNT        17609 a        29557 c        34174 g        19472 t
ORIGIN      
        1 tgatgatgcg cttgacttcc aggccggcga tgcggccggc gtccttggtg gcctgacgct
       61 ggctgtcgtt gaagtaggcg ggcacggtga tgacggcctc ggtgacttcc tcgccgaggt
      121 agtcttcggc ggtcttcttc atcttgcgca gcacttcggc ggagacctgg ggcggggcga
      181 tcttcttgcc gcgcacttcc acccaggcgt cgccgttgtc ggccttggcg atggtgaagg
      241 gcatcaggtc gatgtccttc tgcacttcct tctcttcgaa gcggcggccg atcaggcgct
      301 tgaccgcgta gagggtgttc cgcgcgttgg tgaccgcctg acgcttggcg ggcgcgccga
      361 ccaggatttc gccgtcgtcg gcgtaggcga cgatggacgg cgtggtgcgc gcaccttccg
      421 agttttcgat gaccttgggc ttgccggctt ccatcacgga aacgcagctg ttggtggtgc
      481 cgaggtcgat accgatgatc ttgcccatga gagttccttc agttgggatg ctgttcgagg
      541 ggccgcgcca ggggagaggg ttgcgaccag cttggttcgg atatggggat gcgtccggcg
      601 ctttcaagca gccggttgtg cactgcgcgc cttggacacc accaccagcg cgggacgcag
      661 cacgcggtcg tggaggaggt agcccttctg cagcacgttg atgacggtat tgggctcgcc
      721 gtccgcctcg atggcgctga tggcctggtg gcggttgggg tcgaatttct cgccgagggg
      781 gttgagttcg gtcacgctgg cgccctggaa ggccgacagg agttgcttga gggtcagttc
      841 gacgccttgc cggaggcttt cgagagtggc gttctcgctg gccagggcgg cctccagact
      901 gtctttgacg ggcaacatcg cctgagcgaa tttttcggcg gcgaacttgc ccgccttggc
      961 gatgtcgtcc tgggcacgac ggcggacgtt ttccgtctcg gcgcgggcgc gcaaccaggc
     1021 gtcgtggtgt tcggccgcct tcagttcggc ctggcggagg gcttgttcaa ggcccgggac
     1081 cacgtccaca ggggcttccg gcgcgtcagg tgccgcgggg ggaaggtcgc tgccctggac
     1141 gagttccggt gcgtggtcgg gggtcggcgg ggtggtttgg tcttgcatcg gtggtggctc
     1201 ccagtgttga ccgcacgtaa ctgggggcgg caagcggtat ttcaagaggg gcggaagtgc
     1261 ctaaagtgct gcaatggggt gtaaaatcct gtccgataat ggggtaccgg tcattctttt
     1321 gggtgtattc gtgacgggca ggtatggaca aggctcctga caaggtcggc aaatacaagc
     1381 ttctccggga gatcgggcgg ggcgcgacag cgaccgtcta tctggctgag catccccagt
     1441 acccggatcc cgtggccctc aagctcatcc gctttgacga caagtccaag gatgaggcca
     1501 aatggaaccg gcgcctgctc aaactgctgc gcgcagagat ggacgtctcc cgccgtctcg
     1561 atcaccccaa catcatcaag atcttcgata cggcggtcga caaggacttc gcctacatcg
     1621 tgatggagta ttttccggga acgacgcttg agcactactg tagcttcgag cgcctgttgc
     1681 cgctccaccg tacgatcggc atggtgttca agtgttgcat ggcactcgat tacgcctatc
     1741 accagggtat cgtgcaccgg gacatcaagc cggccaacat ccttgtcgac gagaacgaca
     1801 acgtcaagat caccgacttt ggtctggcgc tcaacatcag caagaagagc gcgtccgatt
     1861 ccaccttcat catgggtgtg ggctcaccgg cttacatgag cccggagcag atcaagggtt
     1921 acccgctcaa tcagaagacc gatctgtttt ctctgggcgt ggtgctgttc cacatgctga
     1981 ctgggcgcct gccgttccgc gccaagaatc ccgcccagct gatctacaag atcatcaacg
     2041 ccgaaccgcc gctggtgtcc cagatcaatc cggaaatccc ggagcagatg gatgccgtga
     2101 tccgcaagtc actcgagaag gatctctact cgcgctacaa gaacggcgcg gatttcgcga
     2161 aggatctgtc ggctgtccgc tacaagatct ttgacgactc ctacgttccg ccggataacc
     2221 gccgcttctc ccagttgcgc aagatgtcct tcttcctgga attcgacgat gtcgagatct
     2281 gggaggtgct gcgcatcagc cgctggcgca gggtggattc gaacacgctc ctgatgcgcg
     2341 aaggcgatgc cgaccagcgc ttcggcatca tcgtggatgg caaggtcgag ctttcggtag
     2401 acggccatcg ggtctgcgaa cttggcgacg gcgagctgtt cggcgaactg gcgttcctcg
     2461 atcagcagga ccacaagcac atgtacaccg tcgtttcgac gacatcactg acctacctgg
     2521 aaatgaatcc cgcggcgctg tcccttgcga ccgaggaatg tcaggacgcg gtccgcagca
     2581 aggtggtcac ggtggcggcc cgtcgcctgg gaaattcgat gcgggagctt tccaaagacg
     2641 gaccgccggc tgagcgcatc accgccagtg ggtttggtat ggaactccag ctggtggaca
     2701 gctgagcacg tgcttgtccg gcgctgccgg ccttgatcac gacacgcttg tcagccaacg
     2761 caccagttca tctgcaccga tcgcgccgga tcggcgcgcc acctcccgtc cgccccgcat
     2821 cagcaccagc gtcggaatgc ttcgaatccc gaagcgggtg gcaaggtcgg gttcgtcctc
     2881 agtattgact ttgcccagcc ggtaggccgg ttcgagtcgc cgggccgcgg ctgcgaacga
     2941 cgcttccatg ctgcgacagg ggccgcacca gggggcccag aaatccacga ccagggggat
     3001 ttcaccgcga ccggcatgaa tccagaaatc ctcgcccgtc agatgcaagg gttcgcccgt
     3061 gaacagcgcc tgcttgcacc gtccgcagcg gccctggtcg cgcagacgtt cgcccggcag
     3121 gcgattgatc gctccacaat gggggcaggg gacgaggatc ggatcaggca tggcaggctc
     3181 caggagcagg atgtccctca tgtggggcgg atcgtgccgg tttcaaccta aagttttgcc
     3241 ttgccgcgcc gacattcctg gcgtagccgc gtgtggatcg cggagtcgac aggaggtggc
     3301 ctgtgagccg aggcagcact tatggaatca gccgggtgga caatgacacg agtcgcaccc
     3361 acggctggct ggtgacgata cagcgtcgcg gcgtgatcta ccgcaagcat ttcagtgacg
     3421 gcgtgttcgg cggcaagcag cgttcctttt ctgccgcaaa ggaataccgc gaccagatca
     3481 tcgccgcgca tccgcccctg tcgatgcagg agtactccaa catcgtcaag aagaacaacc
     3541 gttcgggggt ggtgggggtg tgccgctatt gcgcgtcgga aacccgcgac ctgccggagg
     3601 aaaagcagcg ctggttctgg gtggcctcct ggcccctgcc ggacgggcgc cgcaagcgtg
     3661 tcaagttttc ggtgaagaag tacggtgagg atggcgcatt ccgcatggcg atggatgcgc
     3721 gcgcgatggc gcttgaaaag ctcgagggcg cattcgatcc cggtgccgtg cgtcgcaagt
     3781 cctcccggcg ccgcaccacg ccggttccgg ctaccggagg cgctcccgcg tcacgcaact
     3841 gatcgacaga accggatcct ggtctgtcag tctcccccga gggcgagatt gaccagccag
     3901 gtgaggaggc tgtagagcag ggcacccagg aaggcggtgc cgaagtccgt gaccgtgaag
     3961 ccctgcacca ggccggagac cagccagaac atcaggccat tgatcaccaa cgcaaacagg
     4021 cccagcgtca acacggtgat gggcagggtg atcagcaaca gaagcggtcg caggatcgca
     4081 ttcacgaagc ccagcagcac tgcactgatg agcgccgcag agtagctgtc gacgtggatc
     4141 cctgtgacca cttccgggat cagcatcaag gcgactgcat tgagaaacca gcgcaggatc
     4201 agtttcatca agaggtcctt tcaggtgtca ggtccgcgcc atcagatggt cgcgccgagc
     4261 tgacgggcgc gttccgcgga tgcggcaagc cgcgcgggat cctccgctgg caaatcgagc
     4321 cagtgaccca ggtgacggcg tgcgatggtg cggagcgcgc ggatccagtc gtcccgttcg
     4381 ttgaggcagg gaatgtagcc gaaggccttg ccgcccgcgg ccagaaaggc gtcgcggcat
     4441 tccatgttga tctcctcgag ggtttccaga caatcggcag tgaatccggg gcacatcaca
     4501 tccactgagg tcacgccgtc ccgcgccaat gcctccagcg tagcctgggt atagggctcc
     4561 agccatttcg cgcgaccgaa gcgggactgg aaactgatca cgaggcgctc ggctggcagc
     4621 ttcagggctt cacccagcag gcgggcggtc ttgtggcact cgcagtgata ggggtcgccc
     4681 aggtcgaggc tgcgccgcgg gatgccgtgg aaggacagga cgagcttttc cgcctggccg
     4741 ttgcgttgcc agtgttcccg cacggacgca gccagggcgc cgatgtatcc agggtcgtcc
     4801 gcgaagctgc gtacgtagcg gatctcaggc agattgcgcc agtgctgcag gcagtcgccg
     4861 acggcatcca gcacgctggc tgtggtgctg gcagcgtact gggggtacat gggaatcacc
     4921 aggacgcggg tacagccctg ggcgcgcatt tcatccaggc gactgcgaac agagggttgg
     4981 ccgtagcgca tggcccagtt cacgacgaga tcgggtgcgc cgtcggccac gaagaagccg
     5041 gccagcagtt tggcctgacg ctcggtatgc accttgaggg gcgagccctc gtcggtccac
     5101 acgctggcgt atttgcgggc ggacttggcg gggcgggtgt tgaggatgat gccgttgagg
     5161 atcagccacc agatcggtcg cgggatttcc acgacccgtg ggtcggacag aaactcgcgc
     5221 aggtagggcc gcagcgcgtc cgcagtgggg gctgcaggcg tgcccagatt gacgatgagg
     5281 atgcccgtgc ggcttgggga gccatgccgg aagggcggct cctgccagaa tctggtcatg
     5341 aaggattcct agttctgacc cgaaagggcg ttggtgagca gacgggcggt gatgtccacg
     5401 atggggatga cccgttcgta ggccatgcgc gtggggccga tcacgcccac cgagccgacg
     5461 agttggccgt ccacttcgta gggtgcagtg accacgctgc agtcgtccag gggcgacagg
     5521 ccggcttcgc tgccgatgaa gatctgcacg ccctgggcgc ggttggagat gtcgagcagt
     5581 tgcatgaggc cggtcttctg ctcgaagagc ttgaagagtt cccgcaggcg ggccatgttg
     5641 gaggacaggt cctccgcgtc gagcaggttg gtctcgcccg agatgacgta ggtctgctgg
     5701 tgctcggcaa ccgctgcact gcccgcttcg acggcggcgg tcatcagctc ggtcatgtcg
     5761 gagcgcagct gtttgagctc gtcctgaatg cggatgcgga tctcgtcaaa agaaaggccc
     5821 gcgaagtgct gattgaggta attggccgcc gtgaccagtt cggaggggga atagctgcgt
     5881 ttggtgaaca ggatgcggtt ctgcacgtcg cctgtggcgg tgacgacgat cagcaggatg
     5941 cggccctcgg acaggcgcag gaattcgatc tgacggatgc gggtggattc ctggcgcggc
     6001 gagatgacca cgccggcaaa gtgggtgagg tcggaaagca gctgtgacgc ggcgttgatg
     6061 acccgctggg gttcgccggg ctggatctgg tcacgcaggg tttcgatctg accgctaccg
     6121 accggactga ccttgagcag gctgtccacg aagaaccggt agccccgcgc ggtgggaatc
     6181 cgcccggcgg aggtgtgggg gctggtgata aagcccagtt cctccagatc cgacatcacg
     6241 ttgcgcaccg tcgcgggcga cagctgcagg ccggaaaagc gcgacagcgc ccgcgagccc
     6301 accggctggc cgtcggcgat gtaatgctcc accagggtct tgaggaggat ctgtgaacgt
     6361 tgatcgaggc tgctcatggc gggaaattcg ggggtgacgc gatggattct aaggaaggcc
     6421 gagggtgctg agaagggcaa cgcgctggag ggcccattcg gcacgcagtt ccgaggggtg
     6481 aggcggggcg ttccgtatgg tgatacgttc tcgcacatgc cggtgaatgg cagcgcgttt
     6541 cgccgaatat gtggtttaat cgttgcatgg cccatgcatt cgacactatc gccctgatcg
     6601 gcaaatacca gagcccggaa gtggcggaag ccgtgctgcg gctggccaat tacctgcgcg
     6661 gcctggggct gccggtgatc atcgaacagg gcactgccag cgcgacggga ttgacctccg
     6721 gtttcacggt ggggagttat gaagagatcg gcgtgcgggc cgatctggcg gtggtgctcg
     6781 gcggagacgg gacgctcctg cacagcgcct gccgcctggc cggctacgat gtcccgctgg
     6841 tgggcgtcaa tcaggggcgg ctcggcttcc tgaccgacat ctcccgcgat gtggccctcg
     6901 acaagatcgg cgagatcctc gcggggcgtt tcaccgaaga gcatcgcgtg ctgctggacg
     6961 tggaagtcct gcggggcggc cagcgggtat tccgcaccac ggtgctcaac gatgtggtgg
     7021 tcaataaggg cgagctcggg cgaatgatcg aattcgacct gagggtggac ggggaattcg
     7081 tctacaccca gcgttcggac gggatgatcg tctccacgcc gaccggttcc accgcctacg
     7141 cgctgtcggc caacgggccg atcctgcatc ccagtgtgga cggcattgcc ctcgtgccca
     7201 tgtgtcccca tgccctcacg gcgcggccga tcaccctgcc ggaccgctgc tgcatcgaga
     7261 tcgtcctgct gccgccccac gattcgcgcg tgcactttga cggccagagc cgctatgacg
     7321 cccgcgccgg cgacaccgta cggatcaccc gttcgcccca cacggtgcgc ctgctccatc
     7381 ccgtgggcta cagctatttc gcgatgcttc gcgaaaagct gcactggagt tccacgccgc
     7441 gccacagttg agcgcgctcg ccgtctgccc atgctgcgcc atctgactgt ccgcgatttc
     7501 gtcatcgtcg atcacctcga gctggagttc tgtgccggct ttggcgccct caccggcgaa
     7561 acgggtgccg gcaagtccat cctgctcgac gcgctgggtc tggcgctcgg tggcaagggc
     7621 gacgccggcg tcgtgcgcag cggtcaggcg cgcgcagaga tcgtggcgga attcgatctt
     7681 cccgccgaca gtgcgctagc tgcctggctg gaagaacagt cgctcacgtc cgaggacggc
     7741 actgtccaga tgcgccgggt cattgacagc agcggacgtt ccaaggcctg gctgaacgga
     7801 tcgccggtgg tgctcgggca gttgcgagcg gccggcgagc tgctcgccga catccacggc
     7861 cagcatgccc accacgccct gttgcgaagt gaggcccagc tggcgctggt cgatgcccat
     7921 ggcggcctga gtgccgatgc ggttgaagtg gcccgtcgct tccgtgtctg gatgggggcc
     7981 cgcaaggctc gggaagcaag cgaggcaggc gccgcctcga gcgaacggga gcgcgaactg
     8041 ctcacctggc aggtgaccga gctgcgggaa ctggcattcg aacccgatga atgggacgcc
     8101 ctcaaccagg aacaggccag gctggctcac gcggccagcc tgatcgaagg tagcggggag
     8161 atcctggacg gccttgcgga aggtgagctc gccctggtgt cgctgctgga gcggctggcc
     8221 ggccgggcgg cggacatgcg ccagtacgac gcccgcgtgg gcgatgtgga ggatcttctg
     8281 cggggtgccg gcatccagct cgatgaagcc gtgcatgccc tgcggcgcta tcgggatcgg
     8341 ctcgatctgg atccgcagcg cctgaccgaa gtggatcagc gcattcaggc agtgacggcc
     8401 ctggcgcgca agcatcgttg tgcgccggcg gaccttcccg ccctgctggc cgagagcgac
     8461 gcgcggctcg cccagctgac ccggcagtcc gacccggagg ccctcgcccg cgaagaggcg
     8521 gcggcgcagg aggctttcct cgcggtggcg cggcccttgt cggtggcgcg caagcaggtg
     8581 gcgtcggcgc tgtcggcagc ggtgacggag gcgatgcaga ccctggcgat ggccggtgcc
     8641 cgtttcgagg tgtgcctggc gacagaggtc gcgggggctg cgacggggct tgagcgggtt
     8701 gaattcctgg tcacggccaa tgccagtcag ccgctgcgcc cgctctcaaa ggtggcctcg
     8761 ggcggcgaac tgtcacgcat cgggctggcg atccaggtca tcaccagccg caacgctgcc
     8821 acgcccacgc tgctcttcga cgaggtggac gtgggcattg gcggtcgcgt ggcggagatt
     8881 gtggggcggc tcctgcgcca gctgggcagc gaccgtcagg tgctgtgcgt gacccacctg
     8941 cctcaggtgg cagcccaggc caactggcag tggagcattg ccaaggataa cgacggcgac
     9001 gagaccctgt cacgggtgat ggtgttgacg ccgaccgagc gcatcgaaga gatcgcccgc
     9061 atgctggggg gcgttcagat caccgatact acccgcagcc acgcagcgga aatgctcgct
     9121 gcggcaggca atccggtctg attcccgcgg ttgtcgccgg atcagccagc gagaccggcc
     9181 gcgccggcga aggtgaccag gccggtcagg atgaacacgg ccgctgcggc ccagcggatc
     9241 tgggtgaggg gcaggcggcg ggcgagggct tcgccgagca gcactgccgg cacattggcg
     9301 atcatcatcc cgatcgtggt cccgaggacg acccagccca gtgcatcgaa gcgcgccgcc
     9361 agggcgacgg ttgcgagctg ggtcttgtct cccatctcag ccatgaagaa ggcaatcaga
     9421 gtggtcatga aggcgccggc cgggtgcatg gcggcagggg cttcatcgtc gtccagggtg
     9481 tcgggcttga gggtccacag gccgaagacg acgaacacgg accccacgat ccagggcagc
     9541 cagtgggccg gaataaggtt ggccagccat acgcccaccg agccggccag ggcgtggttg
     9601 aggatcgtgg cgacgaagat tccggcgacg atggcccagg gcttgcgcag ccgcgcggcg
     9661 aggacgaagg acagaagctg cgtcttgtcg ccgatctcgg caagggcgac gagcgccgcg
     9721 gaggtgagga aggcttccat ggagagggcg gcggagtgcc gccggagggt cagatcaggg
     9781 gtgttccgga tttgccgggc ctgtgggggc aatcagtctt cttgcagtcc acgtagagat
     9841 agagggcgtg ttcatgaacc tcgtagccgc gctcgcgggc gatgcggttc tgccgctcct
     9901 cgatttccgg gtcgtagaat tcttcaacct tgccacactg caggcagacg aggtggtcgt
     9961 gatgatggcc ttcgttgagc tcgaagacgg ccttgccgga ttcaaagtaa tggcgttcga
    10021 gcaggccggc ctgctcgaac tgggtgagga cgcggtaaac ggtggccagg ccgatgtcca
    10081 tgtcctcggc gatgagcaga cggtacacgt cttccgcggt gaggtgacga ttgtcggcct
    10141 tctggaagag gtcgaggatc ttgagccgcg gcgtggtggc cttgaggccg atgttcttga
    10201 ggttctggga attgctggac atggtggccc cggtacgtgc acacgctggc ccgcaggagc
    10261 ggccagcccc atatgatata gtttccgccc ttcgttgaga attcggccca gccgggcccg
    10321 tcccgcgacc gccatgcgcc ctctgctctg cctgctgctt gtgctgccga ccctcggtgg
    10381 gtgttcggcg gtcatcgatt ttctcaagcc gtaccggatc gacgtccgcc agggcaattt
    10441 tgtgacccag gagtcggtgt cccagctcaa acccggcatg tcccgcgacc aggtgcgctt
    10501 tcttctgggt acgccgctgc tgaacgatgt cttccatact gatcgctggg attacgtcta
    10561 tcgccttcaa cccggcaagg gcgaggcgca ggagcgtcgc ctgacggtgg tcttcgagga
    10621 cggcaagctg gtgcgtctgg agggcgacgt gcgggcggcc ggcggtgagg gcacggcgac
    10681 caggccgtga cggaccggca tgctgccggt tccctgaaca tgaatttctg aggatgacat
    10741 gagtcaagtc cgtatagcca tcgccggtgc gtccgggcgc atgggtcgca tgctgatcga
    10801 aaccaccctc aaggctgccg acatgaagct ggttgcggcg ttcgagcagc tgggcagccc
    10861 cttcgtcggc aaggacgccg gcgagctggt cggtcaggcc tgcggtgtgg ccatcagctg
    10921 cgacgtggcg gccggtctgg ctgaggccga ctgcctgatc gatttcaccc ggcccgaagg
    10981 gacgttgcat cacctggccc tggcccgggc tgcccgttgc gccatggtga tcggcaccac
    11041 cggcttcggt ccggagggca aggcggccat aggcgcggca gctcaggaca ttccggtcgt
    11101 gttcgcgcca aacatggcgg tgggcgtcaa tgcggtcttc aagcttctgg acgtggcggc
    11161 gcgcatcctc aacgagggct acgacgtgga gatcattgag gcccaccatc gactcaaggt
    11221 ggatgcgcct tccggcaccg ccctgcgcat gggtgaggtg gtggccagcg ctttgggccg
    11281 caatctcgaa gaatgcgcca tttacgggcg tgaaggcgtg acgggcgagc gggacagtca
    11341 gaccatcgga ttcgccacca tccgtggcgg cgacgtggtg ggcgatcaca ccgtgctctt
    11401 cgcgggcatc ggtgagcgta tcgagatcac ccacaagtcg tccagccgga tgtcctatgc
    11461 cacgggcagc ctgcgggccg cgcgcttcct tgctggcaag gattctggcc tgttcgacat
    11521 gcaggacgtg ctcggcttgc gctgacggtc cttcgcgggc gccctcatct caagattcgg
    11581 gcagggcgtc cgttagtcgc aaggtgaagt gagacagggt ccgctgcgtg ctgcgggcct
    11641 gccatcgcca atcctgtgac gcatcgatcg cccgagtgaa tctcccctcc ttacccccgc
    11701 cggcctccag tgacgagctg gtccttgcgc tgcgcgtcct gctgcaagac ctggcgtccc
    11761 gagggtgcat caccatgccg tcggcagcat ctgaagcgaa tccggccgag ctggtagatg
    11821 gcgttcgtgc gctcattgcc gcaaaacagc ggatcgagca gcaataccat gagacacggg
    11881 aacgtctcga cctggcgatg cagaacaccg atgccgggct gtgggactgg gatctggcac
    11941 acgacacgat cgtcctgggc gagcattggc ggcagatcct cggctatgcc cccggcgagg
    12001 gatccgcaga tttcgacggg tggctgcatc gggtgcatcc cgacgacctt caggtgatgc
    12061 gccatcagct ggcggctcac ctgcgcgggg atctggagga ctttgacgcc cagttccgga
    12121 tcgcgacgaa cgccacggcc tggcgctgga tccaggtgcg cggccgcgcc tcgaaccgca
    12181 atgcggccgg acgctggtgc cggctggtgg ggactttccg cgacatcaca cgggtcaagg
    12241 aggcggagct ggagcttctg gatgccaagg agatggcgga agcggccaac cgcaccaaga
    12301 gcgacttcct ggccaacatg agccacgaga tccgcacgcc catgaacggc atcattggca
    12361 tgacggatct ggtgctcgat accgatctcg atccggtgca gcgggactac ctgctcgcgg
    12421 tgaagaactc cgccgacacg ctgctgcaca tcatcaacga cgtcctggat ttctcgaaga
    12481 tcgaagcggg gcgcatgagc ctcgagcaga tcgatttttc ggtcgtggag ttgatggggg
    12541 ccaccatgaa gtccctggcg ctcacggcgt atcagaaggg gatcgagtgc ctgtacaggg
    12601 ttgcacccgc ggtacctgcg gtggtggtgg gcgatccgac gcgggtgcgc caggtgctga
    12661 ccaatctcgt cgggaacgcc atcaagttca ccgagcgtgg cgaggtggtg gtctcggtcg
    12721 atggcgagtc cctcgatggc aacgagttcc tcctccgcgt agcggtgcgc gacaccggca
    12781 ttggcattcc cgccgacaag caggagcagg tgttcggggc tttttcccag gcagacgagt
    12841 ccaccacgcg caagtatggc ggtacgggcc tcggcctggc gatttcacgg cgcattgttc
    12901 aggcaatggg cggcgacctg agcctggaaa gcgaggaggg gcgtggatgc gtgttttcat
    12961 tcacggtccg cctcgggcgt ggcgtccagg cgaacggccg gcctccgccg gtatttgccg
    13021 gccggcgggc cctggtggcg gccgccaacg gtgccgtgcg ggaactgctg cgcgccgagc
    13081 tggagcgggc gggcgtgacg gtccgggagg ccgctgatgg cgaggcgctg gccgcccagc
    13141 tgaagtccgc gcaggatact gcgaagccgt ttgactggtt gatcatggag gccgccatgt
    13201 cggcgcccgg tggctttgcc ctggccgaac agctgcgcaa gggttggccc acgctggatc
    13261 gcctggtggt cgtctccgat cccaaatccc agcgcagtga cgcagaccga tgtgagaagt
    13321 tgcgcatcgg tgcacgcctg atcaaaccgt tctcggcggg cgatctgtcc gaggccttgt
    13381 ccctggcctt gcatgtggac cgactggagc aggtcgatta cctcgaattc cagggcgaac
    13441 agaccgcctg ggagtccgcc ccgaggattg cagagcgcct tgccgtgctg ctggtggagg
    13501 ataaccccgt caatcaggtg gtcgccgtca agctgctcga aaaagcgggg cacacggtca
    13561 ccgtggcggt gaatgggcgc gaggcactgg cccagtatga caagcggcgg tttgacgtga
    13621 tcttcatgga cattcagatg ccggagatgg gcggcatcga ggccacgcag gcaatccgcg
    13681 cacgggaagc acgccgcagc tgggcggccg gccgtcaatc gctgtcggtg ccgatcatcg
    13741 ccatgacggc ccacgcgatg cagggcgaca aggaagcctg cctgatggcc ggcatggacg
    13801 atttcgtcac caagccgatc cgtccgcccg aactttacgc agccatcgcg cgggttcgac
    13861 aggcccagag ctttgccgca tcggacgagc acctggcgga cgcgggcctc ctcaatggcg
    13921 acgcagccat cgagcccgag gagatcgcgg atctgggcgc gacccgggat ttgctggatg
    13981 gcgacgagga tcaattgcag aacctgatcc gcatcttttt caacgatttc gaccgtaacc
    14041 gcaaagcgct cgatgccgca ttggcgacac gggactttgc ccgcctgcgg gatgtggcgc
    14101 acacgctcaa gggttccgcc ggtgtgttca gcgcgtcgcg ggtcgtagcc agcgcggcac
    14161 aggtggaaaa ggcggcgcgt gccgccgacg aggcggaatg ccggcgctgt gtgaatgaat
    14221 tgatgcgcga gctgggtgtt ctggccgggg cgctgcggcg ggcccggcgg ggagggtgaa
    14281 acactgcacc ggattggggc atcgggtggg acgcatttgc ccggagcggt gcctcgggct
    14341 ataatcttgc ggttatcagc gggatagcct cacgggctga tcccgttttt catgtgtagc
    14401 ccggctaccc atgtgagcat ttccatcaaa aagtttcgag gagtccgccg tgtctgcttt
    14461 cccgcccgcc attcttgccc ttgccgacgg gacggtgttt tacggtaaag gtattggagc
    14521 ggtcgggatg accaccggcg aggtggtgtt caacacctcc atgtccggtt atcaggaaat
    14581 cctcaccgat cccagctact gtcgccagat cgtcaccctg acctacccgc atatcggtaa
    14641 caccggcatc aaccaggaag actgcgaggc gggcagggtg tatgccgctg gcctggtgat
    14701 ccgcgatctg ccgctgctgg cgtccaactg gcgcaacgag cagtctctgg atgcctatct
    14761 caaggccgag aacatcgtcg cgattgcggg catcgatacc cgcaagctga cccgcatcct
    14821 gcgtgagaaa ggggcccagg gcggctgcat cgtcaccgcg gcgaccacgg cggagctcag
    14881 ctccgaggcg gccctcgccg aagcccgcgc cttccccggt ctggccggca tggacctggc
    14941 caaggtggtg tcggccgcag aatcctacgc ctggaccgaa ggcgagtggc agctcgacaa
    15001 gggctatgtt ccgcaggaag ccccccgttt ccatgtggtg gcctttgatt acggcgtcaa
    15061 gcgcaacatc ctgcgcatgc tggccgagcg tggctgccgc ctgacggtgg ttcctgccca
    15121 gactcccgcg gcggaggtcc tggccctcaa tccggacggt gtgttcctgt ccaacggtcc
    15181 cggcgatccg gagccctgcg actatgccat cgccgccatc cgcgaagtgc tggccaaggg
    15241 cattcccacc ttcggcatct gcctcgggca ccagcttctc gccctcgctt ccggcgctcg
    15301 caccctcaag atgaagttcg gccaccacgg ggccaatcac ccggtgaagg atctggattc
    15361 cggtcaggtg ctcatcacca gccagaacca cggctttgcc gtcgatgttg caacgctccc
    15421 ggcgaacctt cgcgccaccc atgtgtccct cttcgacggc agtctgcagg ggttggcccg
    15481 cacagacgtg ccggcgttct gcttccaggg tcacccggaa gccagtcccg gtccccacga
    15541 cgtggcctac ctgttcgacc gtttcatcgc cctgatggag ggacggcgcg cgtcctgacg
    15601 caccgacccg gcgggcccca ggttcgccgc tcccggaata ccgctcgcac gcagctgaag
    15661 aatcatgccc aaacgtacag acatccagac cattctcatc atcggcgccg gcccgatcat
    15721 catcggccag gcctgtgaat tcgactattc cggcgcccag gcctgcaagg ccctgcgcga
    15781 agagggttac aaggtcgtgc tggtgaactc caatccggcc acgatcatga ccgatccggg
    15841 gacggccgat gtcacctaca tcgagcccat cacctggcag gtggtcgagc gcatcatcga
    15901 gaaggagcgc cccgatgccg tgctgcccac catgggtggc cagacggcgc tgaactgcgc
    15961 cctcgatctg gcccgccatg gcgtgctcga gaaatacggt gtcgagctga tcggcgccaa
    16021 ggaagaggcc atcgacaagg ccgaggaccg gcagaagttc aaggccgcca tgacccgcat
    16081 cgggctcggc tcggcccgtt caggcatcgc ccacgcgctg gaagaggcgc accaggttca
    16141 ggccgagatc ggcttcccgg tcatcatccg tccgtccttc accctgggcg gcacgggggg
    16201 cggcattgcc tacaacaccg aggaattcgt cgagatctgc aagcgcggcc tggaagcttc
    16261 gcccaccaac gagctgctca tcgaggaatc cctgatcggc tggaaggaat acgagatgga
    16321 agtggtccgc gacaaggcgg acaactgcat catcgtgtgc tccatcgaga acctcgaccc
    16381 gatgggcgtg cacaccggcg actccatcac cgtagcgccg gcccagaccc tgacggacaa
    16441 ggaataccag atcctgcgca acgcctccat cgccgtgctg cgggaaatcg gcgtggatac
    16501 cggcgggtcc aacgtgcagt tcgcgatcaa tcccaaggac ggtcgcatga tcgtcatcga
    16561 gatgaacccg cgggtgtccc gttcgtccgc gctggcctcc aaggcgaccg gcttccccat
    16621 cgccaaggtg gcggccaagc tggccgtggg cttcacgctg gacgaactga aaaacgacat
    16681 caccggcggc gcgaccccgg cgtccttcga gccgtctatc gattacgtgg tcaccaagat
    16741 cccgcgcttc gccttcgaga agttcccgct ggcgaatgac cgcctcacca cccagatgaa
    16801 gtccgtgggc gaggtgatgg ccatgggccg caccttccag gaatccttcc agaaggccct
    16861 gcgcggcctc gagaccggcg cctacggcct ggacgaggtg gaagccgatc aggacgacct
    16921 ggagcgcgaa ctcggcgatc cgggtgccca gcgcatctgg tacgtgggcc aggccttccg
    16981 cgaaggcatg agcctggatc aggtgcatgc gctgacccgc atcgatccgt ggttcctggt
    17041 gcagatcgag gacatcgtca agaccgagaa gaccctcgcc ggacgctccc tgaagggcat
    17101 ttccgccgcc gaactgcgcg agctcaagcg caagggcttc tccgaccggc gcctggccaa
    17161 gctgctcgcc accgacgaaa cctcggtccg cctgcaccgc cataccgccg gcgtgcggcc
    17221 ggtgttcaag cgcgtcgaca cctgtgcggc ggagttcgcc acctccacgg cctacctgta
    17281 ctccacttac gaggaggagt gcgaggccca gcccaccggc cgcaagaaga tcatggtgct
    17341 cggcggcggt ccgaaccgta tcggtcaggg catcgagttc gactactgct gtgtccatgc
    17401 cgccctggcc ctgcgcgagg acgggttcga gaccatcatg gtcaactgca acccggagac
    17461 cgtgtccacc gattacgaca cctccgaccg gctgtatttc gagccgctca ccctggaaga
    17521 catcctcgag atcgtcgccg tcgagaagcc ggttggcgtg atcgtccagt tcggtggcca
    17581 gacgccgctc aagcgcgccc gcgaactcga ggccaacggc gtgcccatca tcggcaccag
    17641 cccggacatg atcgacgccg ccgaggaccg ggagcgcttc cagaagctgc tcttcgaact
    17701 gggcctcaag cagccgccca accgcaccgc gcgcacgccc gaagacgccg tgcggctggc
    17761 cgccgagatc ggctacccgc tggtggtgcg tcccagctac gtgctgggcg gtcgcgccat
    17821 ggaaatcgtc catgagcaga aggacctgga gcgctacatg cgcgaggccg tgaaggtctc
    17881 caacgattcg ccggtgctcc tcgaccgttt cctgaacgat gccaccgagg tggatgtgga
    17941 tgcgctgtcc gacggccacc gcgtcatcat cggcggcatc atggaacaca tcgaacaggc
    18001 cggcgtgcat tctggtgact ccgcctgttc cctgccgccc tacaccctgt cggccaagct
    18061 gcaggatgag ctgcgccgcc agaccgaagc catggcccgc gccctcaacg tggtcggcct
    18121 gatgaacgtg cagtttgcca tccagggtga aggcgacgat gcggtggtgt acgtgcttga
    18181 ggtgaatccc cgtgcgtccc gtaccgtgcc cttcgtctcc aaggcctgca gcctgccgct
    18241 ggcgaagatt gccgcccgct gcatggccgg tcgcagcctg gatgatcagg gcgtcagcgg
    18301 tgaagtggtg ccgccgtatt acgcggtgaa ggaagcggta ttccccttcg tcaagttccc
    18361 gggcgtggat accattctcg ggcccgagat gaagtccacc ggcgaagtca tgggcgtcgg
    18421 ccggaccttc gcggaggcct tcgtcaagag ccagctgggc gccggcgtgc gtctgccgac
    18481 ggccggcaag gccttcatca gtgtcaagca caccgaccgg ccgaaggccg ttgaggtggc
    18541 gcgcgaactg gtcgacttgg gcttctcggt ggtggcgacc cgtggaaccg ccggcgccat
    18601 cgccgccgcc ggtctgcccg tgtcggcggt caacaaggtg aatgaggggc gtccccacgt
    18661 ggtggacatg atcaagaaca acgagatcgc cctggtcatc aacaccgttg aggaaaagcg
    18721 ccaggcggtg accgattcgc gctccatccg cactagcgcg ctggcggcca aggtcaccgt
    18781 gtataccacc atcgaggggg cccgtgcggc gtgcatgggc atgcgtcatc tgtccggcct
    18841 cgaagcctac tcgctgcagg cccttcacgc ggagctgtcc caggcatgag caagattccg
    18901 ctgaccgttg ccggtgctga aaagctgcgt caggaactgc accgtctgaa gaccgtggat
    18961 cgtcccaacg tgatcgctgc gattgcagag gcgcgctccc acggggatct gtccgaaaac
    19021 gccgaatacg atgccgccaa ggaacgtcag ggtttcatcg aaggccgtat ccgcgaggtg
    19081 gaaggcaagc tcggcaacgc ccagatcatc gatcccacgc tgctcgacgc cgacggccgc
    19141 tgcgtgtttg gcgcaacggt ggatctggag gatctggatt ccggcgccaa ggtgagttac
    19201 cagatcgtcg gggacgacga ggcagacatc aaggaaggca agatcgccat cagttcgccc
    19261 atcgcccgtg cgctgatcgg caaatttgcc ggcgatgtgg ctgaagtgca ggcgccgggt
    19321 ggcgtgcgcg aactggaatt gatcgacgtc cgttacgttt gagtggcagc gccatgacgc
    19381 tcggtgcgca gcaatgaccc ggttggcaga acacgtgtac agtctgctca tcaccctgtg
    19441 ggttggcgcc ctgtggacgg tgggctacat ggtggcgccc accttgttcc acatgctgga
    19501 cgaccgcgcc ttggcaggtc gtctggctgg gcagctattt acgtacgtgg gctggttggg
    19561 catggcctcc gcggcctacc tgctggtctt tgtcgcgctt cgcaagaacg gggctgcgtt
    19621 caagagcagc gtgttttggc tggtgctggt gatgctgctt ctgacggttg ccggcgcgtt
    19681 cggtatccag cccgtcctgg ctcagctcaa ggcggaaagc ctgccgcggg aagtgatgga
    19741 aggcgccgtg cgtgaccggt tcgtcgcatg gcacggcgtg tcgagcgtcg tttacctggt
    19801 cgaaagtgtt ctgggcgtgc tgctcgttgt cgtacagggc cgcggtctgc gctgacacgg
    19861 ccatgtgtga cagattcagt cttcccgacg gggcttgcgg tccgacgggg acgcgaacca
    19921 gtaaggcttc ttctgggcgg atttaggctt caaggacgac acgccacggc gtgccgacaa
    19981 gaccttggca gacttggcct tggcgaggga gcgtcgcggt ttatcggtga ccggtgccac
    20041 cgtcttttcg tcttcgcggc gctcacgcca gacgaccagc gtcttgccga tgtgctgtac
    20101 ggcggccgca ccgagttcca cacatacggt cgcgagaatt gcgtcgcgct gggcccggtc
    20161 ctctccatac acgcggatct tgatgagttc gtgggcctgc agggcggcat cgacctcgtt
    20221 caggaccgcc ggcgtaaggc cgttgctggc gacgctgaca accggatgga ggtggtgagc
    20281 ctgcgcgcga aaggcccgcc gctgtgctgg cgtgagttca atcatcattc caactctttg
    20341 aaaaaagggc gtaattatac cgccatgaag cgtagcaaga ccagtaaatc gtggatgatg
    20401 gaacacgtga acgatccttt cgttcagcgc gcaaaaacgg agggaatgcg cgctcgagct
    20461 gcctataagc tgatggaaat cgacgaaaag gacaaactgt tccggagcgg catgaccgtc
    20521 gtggaccttg gtgctgcgcc cgggtcatgg agtcaggtgg ctgtcggcaa ggtcggtggc
    20581 gccggacggg tctttgcact ggatctgctg ccgatggagc cgattccggg cgtgcatttc
    20641 atgcaggggg attttcacga cgacgcattc gtcgaggcgt tcgaagtgca gctcggggat
    20701 gggcaggtgg accttgtgct gtccgacatc gcgccgaata tctcgggaat tccgtccagt
    20761 gatcaggctc ggtcgattca tctggcggag ctggcactag atttcgccgc gcgtcatctg
    20821 gccgccaacg ggcgctttct tgtcaaggtg ttccagggcg aagggttcga tgattaccgc
    20881 aaggcgatgg gtgaagcgtt caggagtgtc gtcgtccgca agccccaggc gtctcgaggt
    20941 cgaagcgcgg aagtctatct gctggggacg ggcaaacggt cctgatccgg cgatcgtgcg
    21001 ccggatgccc gcaaatttga cgatcatttg atccggcgaa cgatcagaca tagaatcagt
    21061 ccactgtacg tccgcccctg ttgggcgatc aaggaatcga gttgaacaac ctcttcaaga
    21121 atctcgcgat ctggttggtg atcggcgttg tgctgatgac cgtcttcaac cagttcaaca
    21181 atcgtcagac tgcccagaat tcggtcgaat actcccagtt catggaggac gccaagcagg
    21241 gcaagatcag caaggctgtc gtcgacggac ggacggtccg tgccacgacc caggacgggc
    21301 gtgtcgtcac ggtttacacg cccggtgtcc aggacatctg gatggtgggt gacctgatgc
    21361 gctacggtgt caaggtcact gcctccaagc ctgaggaaga gcagtccttc ctgatgaaca
    21421 tctttgtttc gtggttcccg atgatcctcc tcattggcgt gtgggtgttc ttcatgcgac
    21481 agatgcaggg cgggggacgg ggcggagcgt tttctttcgg caagtcgaag gccaagatgc
    21541 tggacgaatc cgccaacacg gtgacctttg ctgatgtggc gggctgtgac gaggccaagg
    21601 aagaagtcgg agaacttgtc gacttcctgc gtgatccgtc gaaattccag aagctgggtg
    21661 gccatatccc caagggtgtt ctgatggttg gctcccccgg taccggcaag accttgcttg
    21721 ccaaggccat cgctggtgaa gcgaaagtac ctttcttctc gatctcgggc tccgacttcg
    21781 tcgaaatgtt cgtcggtgtg ggtgccgctc gggtgcgcga catgttcgag caggccaaga
    21841 agcaggcgcc gtgcatcatc ttcatcgacg aaatcgatgc ggtgggccgt cagcggggcg
    21901 ctggtctggg gggcggcaat gacgagcgtg agcagacgct gaaccagctg ctggtggaaa
    21961 tggacggttt cgaaggcgtg tctggagtca tcgtgatcgc tgcgaccaac cgtcctgatg
    22021 tactggatcc tgcgttgctg cgtcctgggc gctttgaccg ccaggtggtg gtgccgcttc
    22081 cggatatccg ggggcgggaa cagatcctcc gtgttcatat gcgcaaggtg cccattgcgc
    22141 cggacgtgga cgctttggtg ctcgcacgtg gctgtcctgg atttgcgggt gcagatctcg
    22201 caaatctggt gaatgaggcc gcattgtttg ccgcgcgagg cagcaagcgc cttgtggata
    22261 tggatgattt tgagcgtgcc aaggacaaga tcatgatggg ggccgaacgg cgatccatgg
    22321 tcatgcccga ggaggagcga cgcaacacgg cttaccatga gtccggccat gccgtcgtgg
    22381 cgaagtgttt aaccaagact gatccggtgc ataaggtgac gatcatcccg cggggccgtg
    22441 ccttgggcgt gacaatgcaa ctgcccgaac aggatcgtta tagccaggac cgtgagcgtc
    22501 tgctccagac cattgccgta ttgttcggcg ggcggatcgc cgaagagatc ttcatgaatc
    22561 agatgaccac aggtgcctcc aacgatttcc agcgagcgac ggatctggcg cgtcgcatgg
    22621 tgacgcaatg ggggatgtca gacaatctgg ggccgatggt atatggtgag gaggaaggcg
    22681 aggtcttcct tgggcgttcg gtgacaaccc ataagaacat gtccgaagcc acgctccaga
    22741 aggtcgacgc ggagatccgg cgtatccttg atcaacagta cgcgctcgcc cggcgtctca
    22801 tcgaggagaa ccgtgagaag gtcgaagcca tgacgaccgc cttgctagag atggaaacga
    22861 tcgactcgga tcagatcaat gacatcatgg acggccgtac gccccgccca ccaaagcctt
    22921 ctcaggtgcc acgccgaccc agtgacgacg cgccaggtgc agcccccacg gcgccggcca
    22981 ctgctgcctg aggcatgtcc gtttcatttt gaggccggcg cagccggcct ctttcgtttg
    23041 ccctctgtct agactgctaa tatgcttctg ggttgtggac gtttccagct cgatcttggc
    23101 cgtccgaaga tcatggccat cgtcaacgtc accgaagatt ccttttccgg cgacggctgt
    23161 aaggcttttg accatgtgct gcgggcggcc gaacgtgcgg tggagcaagg ggcggatctg
    23221 atcgatgtcg gcggtgagtc gtctcggcca ggggctgcag gtgtttcgga ggctgaagaa
    23281 ctggagcgtg tcgttccagt gattgcggct ttgcaggggt tgggggttcc cctgtcggtc
    23341 gacaccgtca agccgcgtgt gatgaaagcg gcactcgacg cgggcgccga tctgatcaat
    23401 gacatcgccg cattccggca gcctggtgca ttggaagcgg tcaaggactc ctctgcggcg
    23461 ctctgtgtca tgcacatgca gggagagccc cgtacgatgc agattgagcc tgtttatatc
    23521 gacgtcgtag acgaggtgcg catctttctg gatgagcgcg tgaaggcgct gcttcaggcc
    23581 ggggttgctg cagagcggat tttggtcgat ccaggatttg gtttcggtaa gacgctggag
    23641 cacaatctgg cgctcctcgc tcggctggag gtcttcggga agtcgggatt tcccgtattg
    23701 gtcggcatgt ctcgcaagtc gatgctcgga gcattgaccg gacgaccggt tggtgagcgg
    23761 caagttgcag gtgcggtcgc tgcgatgtgg gcgattcaac gtggcgcccg cattgtcagg
    23821 acacacgacg ttgcagcgac gcgtgatgtt attcgtgttt tttcggggct tgaagcggca
    23881 gaattccgca tggggcgcca ctgcgaaccc agcgattagg agggaatatg tcaagaagat
    23941 acttcggaac cgacggcgtt cgcggtcggg tgggcgagct tccgatcacg ccggattttg
    24001 gccttcgttt gggttatgcc gctgggaaga cattggttgc gcatgaaaac ctgcgacctg
    24061 gggaacaccc cgcggtcatc atcggcaagg acacccgaat ctcggggtac atgctcgaat
    24121 ctgcgctcga agcgggtttt tcggctgccg gggtggacgt ttacctcgct ggccccctgc
    24181 cgacgcctgc gattgcctac ctcacgcgcg ctttgaggct ccaggtgggg gtcgtgatct
    24241 ccgcttccca caatccgtac gacgacaacg ggatcaagtt cttctccgcg tcgggaacga
    24301 agctgccaga cgcagttgag caggaaatcg aggcgcgtct ggacgaaccc atgggctgtg
    24361 agtcttcggc cgctcttggc aaggcacgtc gcattgatga cgcgcgcgga cgttacgttg
    24421 agttctgcaa gagcaccttc cccaatgaac tcgatctgag aggcatgaag atcgctgtgg
    24481 actgtgccca tggcgctgcc tacatggtgg cgcccaaggt cttccatgag ctcggcgcgg
    24541 aggttatcgc aacaggagtc gagcccaacg gcctcaatat caatgagggt gttggcgcca
    24601 catcaccaac gcatttgcgc gataccgtcc tggcgagcgg agcggacatc ggcatcgcgc
    24661 ttgacggcga tggcgaccgg atcatgatgg ctgattcagc cggcaacctg tacgacggtg
    24721 acaagttgct gtatgtgatc gccaaagcgg cgctggtcgc agggtctccc ggagccgggg
    24781 tcgtcgggac gctgatgagc aatctgggat tcgagcatgc tattgcccgt ctagggctgc
    24841 cgttcgcgcg tgcaaaggtg ggtgatcgct atgtcctgga gcagatgcac gagcgtcggt
    24901 ggcgcctggg cggggagaat tcagggcaca tcatctgcct cgataagcac acgaccggag
    24961 atgggattgt cgcggggctt caggtgctgg ccgcattgcg taagcaggga ctcacgctgg
    25021 agcagatgtg cgaggacctc gagttttatc cacagaagct cgtcaatgtg ccattgcgtt
    25081 ccgggttcgt ttggacagac agccgcgaga ttgctcgagc agtcgatgac gcgaaggcga
    25141 cactcggtca gtccggacgc gtgttgctgc gtccgtccgg gaccgaaccg ctcctccgtg
    25201 tcatggtcga ggggcaggat gcccggatcg ttgaacagct cgcccatggg attgcggaag
    25261 ttgtgcgtgc cgctgccatg tgacgacacg caccaagggg cgtaacgtcc caggcgcaca
    25321 gtaaacacgg attaagtgat gctttgacgg ctgggcgctt gttgcactaa aatcgaaaaa
    25381 tttgtccgag tggaagtcgt gcggaaaaaa ctggtggttg gcaactggaa aatgaacgga
    25441 gggctggggc agaatgcgtc cctactttca tcattgcgtc aggctgaagc cctcgcgggt
    25501 gtggacgtgg cggtttgtgt gccattccct tacctttccc aggcacagca gatgctgagc
    25561 cagtcctcgg tcgcctgggg ggcgcaggat ctcaacatgc atgattctgg tgcattcacc
    25621 ggtgaagtga gtggtgggat gttgagggat ttttcctgta gctgggtgat tgttgggcac
    25681 tcggagcgac gcgctctgtt cggagagggg gatgacgttg taggccgaaa agtgcgcgca
    25741 gcccttcggg ttgggttaag cccgattctg tgcgtaggag aagcgctggc tgagcgtcgt
    25801 tcgggtgttg ctgaatcggt ggtgaaacgg cagctaagtg tgttagccgg gctgagtgct
    25861 gacgagttgg agcgcgtggt cgtggcatac gagcctatct gggccattgg tacgggtgta
    25921 acggcctcac cggaagaggt gcggtcaatg catcgtctca tccgtcaatg gctgggatct
    25981 atgcatggag agcatgtggt cgacaagctt cgtattctgt acggggggag tgtgacggct
    26041 gaaaacgccg cactgctgtt ctcgcaggat gatgtagatg gtgggcttgt cggtggggca
    26101 tccttgaagt tcgatagctt ttatgccgtg tgtcaggccg cgtcggtcgg tacaggatcg
    26161 ggagagattt gtgggtaaca tgatgttttc gttggtactt gtggtgcacg tgcttgcagg
    26221 gctcggtgtc atcggactgg tgttggtgca gcacggcaaa ggagccgaca tgggcgcggc
    26281 atttggcagt ggtgcttcgg gtagcctctt cggttcgtca ggttcggcta actttctgag
    26341 tcggacgaca ggtgtgcttg ctgctgtatt cttcgtgacg agtctgggtc tgagctatct
    26401 tgcgggtggt gccgagcgga agcagcccac aagcgtcatg gagggggtgg tccacccgcc
    26461 tgtatcaggc ggtgtcgcca caccgccccc tccgccgatc gaagacgcat ccaaggcaaa
    26521 agacattcct cgatagtttt aagtaatgct tggcgctgcg gtcgggttgt cttataatcc
    26581 gctgagtcaa acagacgtgc cgacgtggtg aaattggtag acacgctatc ttgagggggt
    26641 agtggcgaga gccgtatgag ttcgagtctc atcgtcggca ccagcacgaa taagacagca
    26701 agccgcccgc acatgcgggc gtttgcttgc aaatagcagt aaatttgctg gtcatcagtt
    26761 gcggggggtc aagtgatgtt ggaagcgtat tttccagttc tcgtgtttat cctgtttgcg
    26821 gctgggttcg gattgatccc gatcttcgca ggctggattt tggggcccag caagcctgat
    26881 gccgaaaaac tctccccgtt cgagtgcggc ttcgagccct tcgaagatgc gcgtatcaag
    26941 tttgatgtgc gctactatct gcttgcaatc ctctttattc tcttcgatct ggaagtggcc
    27001 ttccttttcc catgggcgac cattctccgg gagattgctg gtactgaggc ggtccggtcg
    27061 ttcggattct tcgagatgct ggtctttctg ggcattcttc tggttggcta catctacgtc
    27121 tggaaaaaag gtgcgctcga ttgggagtga tcttttggcg gaatagaggc tgatcgtccg
    27181 tcccgatagc gcactcgttc ttaaaggggt tttatgagca tcgaaggcgt tttcaaggaa
    27241 gggtttgtaa ccacttccct tgataccgtg atcaactgga cgcgtacggg ttccctttgg
    27301 ccgatgacct ttggtctggc ctgttgtgcg gttgaaatga tccatgctgg ctgttcccgg
    27361 tacgaccttg atcgatttgg tgttgtcttt cgtcctagcc cccggcagtc cgacctgatg
    27421 atcgtggccg gtacgctctg caacaagatg gcgccggctt tgcgcaaggt ttatgaccag
    27481 atgtctgagc cgcgttgggt tatttcgatg ggatcttgtg cgaatggtgg tggctactat
    27541 cactactcgt attccgttgt tcgtggctgt gatcggattg ttccagtgga cgtctacgtt
    27601 cccggctgtc ctcctacggc ggaagcgctg ttgtacggaa tcgtgcagtt gcagaacaag
    27661 atcaagcgcg aatgtactat tgctcggtaa cggcaggcga tcatgagttc taagctcgaa
    27721 aatttgaccc gatgcctcag cgaaaaattt gcagacgaca tcgtgcgtct catagacgct
    27781 gccggcgaag tgacgctcga agtacgctct gaaaattatt tggctgtggc ggagtccttg
    27841 cgggatgatc cagaactcgc atttgaacag ctggtagatt taacgggtgt agattattca
    27901 gtgcatgcat tggctgattc tttttcggga cgctttgcgg tggtttcgca ccttctttcg
    27961 attcgccaca atgtacgtct ccgccttcgg acttttgccg aagattcgga tttccccatg
    28021 gtcgcttccc tttgtgcggt ctggcccagc gcgaattggt tcgagcgcga ggcctttgat
    28081 ctctttggcg tgatgttcat ggggcatccg gatctgcgcc ggatcctgac tgactatggg
    28141 tttgttggtc atccgttccg caaagatttt ccggtgtcgg gctttgtgga aatgcgctac
    28201 gaccaggatt tggggcgtgt gatctaccag cctgtctcca tagaaccgcg agaaaataca
    28261 cctagaatcg tgcgcgaaga ttcctacggg gatgttggtc atggctgaaa ttcgtaacta
    28321 cacgatcaac tttggaccac agcacccgtc ggcgcacggc gtgctccgat tggtattgga
    28381 gcttgacggc gaggttgtcg agcgtgcgga tcctcatatc ggacttcttc atcggggaac
    28441 ggagaagctt gccgaaacca ggacatggat tcaatctgtt ccgtacatgg atcggcttga
    28501 ctacgtctcc atgatgtgca acgagcacgc ttattgcatg gctattgagc gtcttctcgg
    28561 attggaagtg ccgctccggg cacaatacat tcgtgtgatg tacgatgaaa tcacgcggat
    28621 tttgaatcac cttttggcag tcggaactca cgcactcgac atcggggcga tgaccatggt
    28681 tctttacacg ttcagagagc gtgaagatct catggacgca tatgaagccg tatcgggtgc
    28741 tcgaatgcat gcggcatact accgcccagg tggtgtttat cgggatttgc ctgatcgcat
    28801 gccgcagtat gaagtatcca agtggaaaag cgaaaagatc gttcgtgagc tcaatgagaa
    28861 tcgtcagggc tcactgctcg attttcttga agatttctgt gaacgattcc cccgttatgt
    28921 tgatgaatac gagactctgc tgacggataa ccgaatctgg aagcagcgga cggttgggat
    28981 cggtgttgtt tcgccggagc aggccttggc gcttggattc accggtgtga tgttgcgtgg
    29041 ttccggcatc gaatgggatt taagaaaaaa gcagccgtac gaagtttaca gccaaatgga
    29101 cttcgacatt ccgataggaa cggctggtga ctgctacgac cgttacttgt gtcgcatcga
    29161 agaaatgcgt caatccaatc ggattgtcaa gcaatgcatc gaatggcttc gcaaaaaccc
    29221 cggcccggta atatccgata atcacaaggt agcccctcct gcacgagagc agatgaaggc
    29281 aaacatggaa gagctgattc accatttcaa gctcttcacg gaaggtattc atgttccagc
    29341 gggtgaggtg tatgcgcccg ttgaacaccc caagggagag tttggggtat atgccatctc
    29401 tgacggtgca aataaaccgt acaggttgaa gttacgctca cctggatttg cacatttgtc
    29461 ggcgatggat gcaatgtcac gaggacacat gattgcagac gtcgttgcaa tcattggcac
    29521 gatggacatc gtgtttggcg acgtagaccg gtaaatgtaa gccatatgtt gatccaagtg
    29581 gtgtaaacag tattcttgca ggcagacgaa cggaatatgc ttagtcctga atctttgaaa
    29641 aaaattgatc gcgagatagc taaatatcct gttgatcaga agcagtcggc ttgcatgtct
    29701 gcattgcgaa ttgcgcagat ggaaaaaggc tggctagcca aagagacgat cgagttcgtt
    29761 gctcggtatc tcgatatgcc ttcaattgcg gcttacgagg ttgcgacgtt ttacaacatg
    29821 tacgatctga cgccagttgg cgcaaaaaaa attaccattt gcaccaactt gccgtgcgtt
    29881 ctctccggcg gcgtacatgc agctgagtac ctgaagaaga aactcgggat tgattttaac
    29941 gaaacgactg ctgatgggct ttttacgctg aaagaagggg aatgcatggg tgcatgtggt
    30001 gacgcaccag tacttctctt aaacaaccat cgcatgtgca gttggatgac ggaagagaaa
    30061 atcgatcaac ttctggctga tcttaaaaaa tgagcgctca aggcattatt ctctctgggc
    30121 ttgacggcga caggacttgg cgcctcaccg actatctaaa gcgcggtgga tatgaggcgc
    30181 ttaaaaagat tattgccgat aaaattccgg ctgatcaaat cattgctgag gtcaaaaaat
    30241 ctgcgctgcg cggtcggggt ggtgccgggt ttccaacggg tttgaaatgg agtttcatgc
    30301 caagggcgtt ccctggcgcg aagtatatcg cctgtaactc cgatgagggt gagcccggca
    30361 cgttcaagga tcgtgacatc cttcggttta atccccattc agtaattgag ggtatggtga
    30421 tcgccggata tgcgatgggg tgcgagcggg gttacaacta tattcacgga gaaattttcg
    30481 agacctacga gcgtttcgaa gaggctctgc aagaggcgcg ggacgctggg cttcttggga
    30541 aaaacattct tggctcggat tttagttttg agctcttcgc tcatcatggc tacggggcct
    30601 atatttgtgg tgaagagacg gcattgctag agtcgatcga aggaaagaaa gggcagcctc
    30661 gtttcaagcc gccttttccg gctagttttg gtctgtacgg gaagccaacg acgatcaaca
    30721 acactgagac atttgcttca attccataca tcattcgcga gggcggggac gcgttccttg
    30781 cgctgggaaa gcctaataac ggcggcacaa agctcttttc agtttctggt catgtaaatc
    30841 ggccaggaaa ttacgagatc ccgcttggta caccttttcc cgtgctgctc gaaatggcag
    30901 gcgggatgcg tggtgggcgt aaactgaagg gtgtaattcc aggcgggtcg tctgctccgg
    30961 tgctgccggg ccacgttatg atggattgta cgatggacta tgactcgatt tccaaggcag
    31021 gatcgatgtt gggttctggc gctgtgatcg tcatggacga gtccacctgt atggtgaagt
    31081 cccttgaacg gctctcttac ttttactttg aagagtcgtg tggccaatgt acgccatgcc
    31141 gggaagggac aggctggtta tatagggttg tccatcgaat tgagaacggc ttgggacgtc
    31201 ccgatgatct cgatttgttg aatagtgtta cagcaaacat catgggcaga acgatctgcg
    31261 ccttgggaga tgctgcgtca atgccagttc aaagctttat caagcacttc ggcgacgaat
    31321 tcgcctacca cattgaacac aaaaaatgtc tcgtgccggt tgatgttcag cgcgaaggca
    31381 gcaagatttt cgtgggctcg acatgctaga aatcgaaatt gacggcaagc aattgcaggt
    31441 tcctgacgga agtaccgtca tagaggctgc aagtaaagtg ggtgcgtacg ttccccattt
    31501 ctgctatcac aagaagctgt ccattgctgc gaactgccgg atgtgccttg ttcaggttga
    31561 aaaggctccc aagccattgc ctgcttgtgc aactccggta acaaacggca tgaaggtatg
    31621 gactcattct gagcctgcga ttaaggctca gaaaggtgtg atggagttcc tgcttatcaa
    31681 tcacccgctt gattgcccaa tctgcgacca aggtggcgaa tgtcagttgc aggatctatc
    31741 tgtcggatat ggtggcgttt cctcgcgcta ccaggaagaa aagcgagtcg tctcgaataa
    31801 ggatcttggt tccctcgtat caaccgatat gacgaggtgc atcaactgca cgcgctgcgt
    31861 tcgatttacg acggaaatcg cggggttgat ggagctcggg caggcattcc ggggtgagca
    31921 cgccgagatc atgccttttg tggaaaaggc gcttgaatct gagttgtcag gtaacattat
    31981 cgacctgtgt ccggttgggg cacttacgtc gaagccgttc cgttttggcg cacgcgcttg
    32041 ggagatgtcg cgcaggaagt ctgtgagccc gcacgattct ttggggtcga atctgatcgt
    32101 tcagattaag aacgataaag tcaaacgcgt tttgccgctt gaaaacgagg caattaacga
    32161 atgctggctt tccgacaggg atcgtttctc ttacgaggca gtgaatggac ctgagcggct
    32221 tctcaaaccg atgatcaagc aaggtggggt gtggcaggaa gcgacttggg aagtggcgct
    32281 cgagtacgtc gtcaacgggc tgaagtccgt ttccaaggag cacgggccta agtcaatagg
    32341 ggcctggatt tccccgcaca gtacgcttga ggaaatgtat cttgctcagc gcttgatgcg
    32401 gggactggag tccgggaacg tggacttccg gcttaggcag tctgatttct cggctgatga
    32461 taagcttgaa ggggcgccat ggctcggcat gtcgatcgca gatgtatcga aactggaccg
    32521 cttgttgatt gttggctctt tctttcgtaa ggatcatccg ctgcttgcgc agcgggtccg
    32581 tcaagcagcg aagaaggggc ttaagatcaa cgcattggga gcggcatttg atgactggag
    32641 attgccgctc gcaaatgtct gcaaagtagc tccagatgaa ttcaaggttg tactggcgcg
    32701 tattgccggt cgaattgctt ctcaaaccag tcagtccatc agcgacgagc tggccagttt
    32761 tgtaagcgag tctggcgctg ataatgaatc gagcgcaatt gcagattcta ttgccagtgg
    32821 acgcaagatc gcaatctggc tcggcaatca ggccgcgcag gacgtggaat ctgttcaact
    32881 tcagatcctg attcaagaga ttgcacgtct ctcacgcgcg agcttcggcg ttattggcga
    32941 agcggcaaat agcgtaggcg ggtacattgc tggggcgatt cctcatgccg atttggcagg
    33001 gatgaacgtc cagcagatga tcaatggaga tcttaaagca tgtgtgttac tgggtgttga
    33061 gcccgaatat gacatggctg acggagttgc tgcgcggact gctttgcaga acgcagaaac
    33121 tgtcatcgtc ctaaatgcgt ttaaatccaa agatgccctg gactacgctg acgtcatgtt
    33181 gcctgtcgct ccatttacgg agaccgcagg tacatacgtg aatgccgaag gcgcaattca
    33241 atcattttat tcggtggtta agagtacggg tgattctcgt ccggcatgga aaattcttcg
    33301 agttctgggc tcaatggcag gtattcctgg atttgagttt gaacgtatcg aagatgtgcg
    33361 tgacgctagt ttgggcccta atcatgcaaa tgttaagttg agtaatgttc tgaaaggatg
    33421 cgttgtgacg gctaagcgta aggcttgctc attgcagcga gtttcagaca ttccgcctta
    33481 tgctgctgat ccgatcgttc gtcggtccga acctcttcaa aatacattgg atgcagttat
    33541 tcctactgca agaatgtcca gcaagacact cgcggaaatt ggcgtgggtg acggggagaa
    33601 agtacgcgtt aagtcgaagt ctggcgaagt gacgattttt gcccaggttg atagcggtgt
    33661 tgcagatggt tgcgtacgac ttgccggtgc tcatgatgcg accaagctac ttggcagtct
    33721 tagcgctgaa attttggtgg agcgtgcctg atggaaaatg tcctgcagcc cgttgctgaa
    33781 tttttcgggg atgtttggcc tttggtctgg agcctagcga aaattgtcgg gatcattgct
    33841 cccttgtttt tgtgtgtggc atatctcact ctggctgaac gcaaggtgat cggttacatg
    33901 caggtacgaa ttgggcccaa tcgggttgga cccaaaggat tgcttcaacc gatcgcggac
    33961 ggtgtaaagc tcttgcttaa ggaaataatc gttcctagtt gcgccagcaa gagtttgttt
    34021 attgttgggc cagtgctagc gttggggccg gcgcttgctg cgtgggccgt agtgccattt
    34081 gcagatgggt gggtagttgg taatgttgat gcgggtttgc tgttccttct agctattaca
    34141 tcaattgaag tctacggagt aatcatcgcg gcatgggcat caaactccaa gtatgctttc
    34201 cttgggggga tgcgggcggc ggcgcagatg gtttcgtatg aaatctctat ggggttcgct
    34261 ctggtgagtg tcctaatgat ttcatcgagc ttgaatttga cagatatcgt cagtagtcaa
    34321 ggcaagggtc agtgggccga ttggggtttt ggcttcctta gctggaactg gctgccgctt
    34381 tttccaatgt tcttggttta tctgatttcc ggggttgcgg aaacaaatcg tgcgccgttc
    34441 gatgttgtcg aaggagagtc cgagattgtt gcgggacaca tgattgagta ttcgggaatg
    34501 gcgttcgcac tgttcttcct tggtgagtac gcgaacatga ttcttatctc cgcgctcacg
    34561 tctatcctgt ttctcggggg atggttgtct ccgttcgggt ttctcccaga cagctttgtt
    34621 tggttggcac ttaaaacatc cttcatcctg tttctattcc tttggttccg cgcgacattt
    34681 ccgcggttca gatatgacca tatcatgcgt ctgggttgga aggtatttat tccggtaaca
    34741 cttttctggg tgatgcttgt tgctgtttgg atcatgtctc ccctgtcggt ttggaagtga
    34801 ggggtaaatt atgggttcta tgaaggattt tgtaggcagt ctttttttaa aggaactgct
    34861 gaaaggaatg gctcttacag ggcggcatct ctttgcacgt aagattaccg ttcagtttcc
    34921 agaagaaaag acaccacaga gccctcgttt tcgtggccta catgcattgc gccggtatcc
    34981 gaatggcgaa gaacgctgta ttgcatgtaa gctttgtgag gctgtttgtc ctgctatggc
    35041 gatcaccatc gaatctgaac agcggggtga tggatcccgt cgcacaagtc gatacgacat
    35101 cgatttgacg aagtgcatat tttgtgggtt ttgtgaagag gcgtgccccg ttgatgcggt
    35161 ggttgaaacc cgaatatttg aatatcatgg cgaaaagcgg ggcgatcttt attacacgaa
    35221 acagatgctt cttgctgtgg gcgaaaaaca cgagaagcag attgctgccg atcgtgaagt
    35281 tgaatccaag taccgctgat tgtagtcaat tggcaaccag gatatagaca tggattttca
    35341 ggtggtagtt ttctatttct tctcggcgat catggtgata gcttcgctga aggtaattac
    35401 gtcccggaat ccggtacatg ccgctttgta cttggtgctc tcctttgttt cagcaagcgg
    35461 gatttggttg ctgctgcagg ctgagttttt ggcgatagcg ctggtgatgg tttatgtcgg
    35521 tgcagtgatg gtgctgttcc tgttcgttgt gatgatgctc gacataaaca tcgaaaggct
    35581 tcggcaggga ttctggagct atctcccggt tggtgcgttg attgcgattg caatggtcgt
    35641 cgaaatggga atggttctgg gcacttattt cggacttgac tccgtacctt tgcctcctgc
    35701 gcctgagcag ggatacagca atacaaaggc gttgggtagg cttctattca cggagtacgc
    35761 ttatccgttt gagcttgcct cgattgtgct tcttgttgca atgatcgctg ctgtggcgct
    35821 gaccttgcgt aagcgagata gtgttaagaa agttgacccg tctgctcagg tggcagttca
    35881 gcgaaatgat cgcctacgga ttgtttcgat gccttctgat tcagaataat taaagcgtta
    35941 attaagacct cgggattgct gatgctgcca ctttctcatt atctgatttt gggtgccatt
    36001 ctctttgcga tcagcgtagt tggcatattc ttgaacagaa agaatctgat cgttcttttg
    36061 atggcgattg aactaatgct tctggcggta aatattaact tcgtagcgtt ttctcgttat
    36121 ctgggagatg cagcggggca aatcttcgtg ttttttgttc tgactgtcgc tgccgctgag
    36181 tcagggatag gtcttgccat cttggtggct ctgttccgaa atctgcgcac gattcatgtt
    36241 gatgatctgg atactctcaa gggataagga agctttttga tgtcggatat gcaaaagctc
    36301 tacctcgtag ttccgctcgc tccgcttttg ggtgcagtca ttgctggtct ttttggaaag
    36361 gccatcgggc gggcaggggc acatacagtc actatccttg gtgtggctat ctcgtttgtc
    36421 tgctcggttt tcattttcaa tgacgtggct gcaggcaata ccttcaacgg aacgttatac
    36481 acatgggcaa caagtggtgc tttgacgttt gaagtcggat tcctaatcga tactctgacg
    36541 gtcatgatga tgatcgttgt caccttcgtg tccttgatgg tgcacgtgta tacgataggc
    36601 tatatgactg atgatcctgg ctaccagcgt ttcttcagct atatctcgct tttcacgttt
    36661 tcgatgctga tgctggtcat gtccaacaat ttcctacagc ttttctttgg ctgggaggct
    36721 gttggtctgg tttcctacct tctgatcgga ttctggtata cccgtcccac cgcgatcttc
    36781 gcaaacatga aggcttttct tgttaatcgt gttggcgatt ttggttttct acttggcatc
    36841 ggattgattg cagcctacgc ggggagcctc aactacgcag aagtgttcaa caaggcggga
    36901 gatcttgctg gaatgactgt tccggcaacg ggttggccat tgatcaccgc agtctgtatc
    36961 ggtctcttca tcggtgcaat gggcaaatct gcgcaggttc ccttgcacgt ctggctgccg
    37021 gattccatgg aaggtcctac gccgatttca gcgttgattc atgctgcgac gatggtgacc
    37081 gcaggtattt tcatggtcgc gcgcatgtcc ccgttgtttg aactgtcgga taccgcgctc
    37141 agcttcgttc tgatcatcgg tgcaacgacg gcattgttca tgggctttct tggcattgtt
    37201 caaaatgata tcaagcgtgt tgttgcgtat tcgacacttt ctcagctggg ttatatgaca
    37261 gttgcgctgg gtgtttctgc atactctgct gcggtattcc atctgatgac ccatgcgttc
    37321 ttcaaggcgc ttctgttcct tggagctggt tcggttatta tcggaatgca tcatgatcag
    37381 gatatgcgca acatgggcgg cctctggaag tatatgcctg ttacctggat cacatctctt
    37441 gttggctcat tggccttaat cgggtttccg tttctctctg gtttctattc taaggactcg
    37501 atcattgagg cggttggcgt gtcgcatttg gcagggtcag gctatgcata catatgtgtg
    37561 atcgttgggg tattcgttac tgccttttac tcctttcgca tgtatttcct tgtcttccac
    37621 ggcaaagagc ggtttggatt ggtgcatgag agccatcacg atgttcatca ccacgatgat
    37681 ggtgatgcat cgcatgacga agatcaccat cacggcctcg cacctgggca aaagccccac
    37741 gagtctcctt gggtcgtaac agtccctctc gtcatgctcg ccatcccttc gatgttggtt
    37801 gggttttaca caatcgaacc gatgttgttt ggagaatact tcaagggtgt aatttacttt
    37861 tccgatgcgc acccggttat ggctgaactg gaggaggagt tccacggggc cgttgcaatg
    37921 gcattgcacg gattcacgac tttgccgttc ttccttgcca tgagcggtgt cgtgttagct
    37981 tggttccttt atcttgtcca gccgtggatt cccgaaaagg ttatgctggc ctttaagccc
    38041 attcacacat tgcttgaaaa taagtattac tttgataagt ttaatgaaac cgttttcgcg
    38101 ggcggggcac gtttgttggg caagggtatg tggaaagctg gtgatcaggc gatcatcgat
    38161 ggaatggtgg tgaacggttc agccaaattt gtcggatgga ttgctcagct caccaggctc
    38221 ttccagagcg gtcatatcta ccagtatgcg ttttccatga tcatcggcgt ttttgtactc
    38281 ctcgtttttt ggatcaatcg gggttgacgg tcggactgga atatacagga cgcagcagac
    38341 catttgatga actgatgcac cgtaaggtgt agatgacgga aatttaagat gacgggtatt
    38401 ccattcctca gcctcgcgat ttgggtgccg atcgtcggcg gcctggtggc cctggcgaca
    38461 ggttccgaca agaatgcacc ggtagcgagg tttgttgcgc tgtttactgc tcttgtgggg
    38521 ctcgcggtca gtattccgct cttcactggt ttcgacgtcg ctacaagttc tatgcagttt
    38581 gttgaagctt atgcttggat tccgcgtttc aacatcaatt actatcttgg cgttgatggc
    38641 atctccgtgt tgttcgtggt tctgaatagt tttatcactg ttatcgtagt gattgcaggc
    38701 tgggatgtga ttcaggaaaa ggtttcccag tacatggccg gattcctgat tatgtcgggt
    38761 cttataaacg gcatatttac tgcgctggat ggtgttctgt tttacgtgtt ctttgaggcc
    38821 tctctgattc cgttgtttat catcatcggt gtatggggtg ggcctaatcg agtttacgcg
    38881 gccattaagt tttttctcta cacgctagcg ggttcgcttt taatgttggt cgcactgctt
    38941 tacctcttca tggagtcggg tggtagcttc aatattctcg aatggcacga gcttaagctc
    39001 gggatggatg tccagacctt gattttttgg gcgtttctgg tgtcgttcgc agttaaggtc
    39061 cctatgtttc cggtccatac ctggctgcct gatgctcacg ttgaggcgcc tacagggggt
    39121 tccatcgttc tggcagcgat tggtctcaag cttggggcgt acggttttct tcgattttcg
    39181 ctgcctatca cgccggacgc ggcacaagct tttgctccga tgatgattac gctttccctg
    39241 attgccgtca tctatatcgg attcgtggcg ctcgttcaga ccgacatgaa gaaattggtc
    39301 gcatattcgt caatctcgca tatgggattt gtaacgctcg gattctttat ttttaacccg
    39361 ctcggcgtcg aaggtgcgct cgtccagatg atctcgcatg gatttgtttc cggtgcgatg
    39421 tttgcgtgta ttggcgtgct gtacgaccga atgcattcgc gccagattgc tgattatggt
    39481 ggcgttgtga acgtgatgcc caagtttgca gcgctgttca tgttgtttgc gatggcgaat
    39541 tccggacttc cagcaacctc tgggtttgta ggggaattca tggttgcatt gggtgcggtt
    39601 cagcacaact tttgggtagg ttttgctgcg gcgacgacgc tgatacttgg ggcggcgtac
    39661 acgctgtgga tgtacaagcg cgtggtgttt ggcgctattg ctaacgatca tgttgctgaa
    39721 ctgaaggaca tcaataatcg agagttcgtt tttcttcttg ttcttgccgt gtgtgttctt
    39781 gcaatgggga tttacccctt ccccgtcacg aacgttatgc atgcatcggt cagcgatctg
    39841 ctgaaacatg ttgcggctgg caagttgtaa tcacgggttg tcattgaatt cttgcagagt
    39901 ggtccaataa tgaatttcgt cactcccgat ttctacccgg ctgcgtcaga gctgtttgtt
    39961 gcctgcatgg ctatcgttgt gctcatggcg acgacattcg ctaaagatgc tcgcagcgtt
    40021 gggtactggt tgtcagtcct taccttggtc ggggcagcgc tcgtcacctt gttcaccatg
    40081 gatggtcagg tttcgttcac gttcagcaat atgtacgtgg atgacctgat gggcgacttc
    40141 ctaaagctga tgctttattt tgcggtactt gtcgttctga tttactcaag gggttacatg
    40201 aatgatcgca acatcgaatc ccctgagttt tatctactgg ttattttcgc aacgcttggc
    40261 atgatggtga tggtgtcagc aaatcacttc ctgacgcttt atcttggtct tgaattgctt
    40321 tcgcttgcgc tgtatgcaat ggttgctttg cagagggatt ccgtccgttg tacggaagct
    40381 gcaatgaagt actttgttct tggggcgcta gcgtctggtt tgctgctcta cgggatttcg
    40441 atggtttacg gtgcgacagg aacgcttgag atttctggtg tcgcgcaggc ggtttatcac
    40501 caggcgggca atcgtacggt tctcatgttc ggccttgtct ttctcgtcgc cgggctggca
    40561 ttcaagctgg gcgttgttcc ctttcatatg tggattcccg acgtatatca gggtgcgcct
    40621 acgtcggtga cgctcttgat cgcttcggcg cccaagcttg ctgcgttcgc tatggccatg
    40681 aggttgctgg tgaatggatt gtttgatctt gcagatcaat ggcagtctat gctgatgttt
    40741 atggcagttc tatccattgt tcttggtaac cttgcggcga ttgcccagac gaatctgaag
    40801 cggatgctcg gttattctgg catctcccat atgggcttta tgctgctcgg gctggtctca
    40861 ggtgttgtag gcggggatcg acattttgct ctgaatgcat acagctcgtc tatgttttat
    40921 gttgttgctt acgtgctgat gagcctcgcg tcgtttgggg ttttgattct catgtcgcgt
    40981 gtgggattcg aggctgagac gcttgatgat ttcaaggggc tcaataagcg caattcgtgg
    41041 ttcgctggtg tgatggcgat atctatgttt tcgatggccg ggatcccttt ctttatcgga
    41101 ttttttgcga agttttcggt gcttcaggct gtcgtcgcgg ccggctacat cggggttgcg
    41161 attcttgcgg ttgttatgtc gttgatcggc gcttattact atctacgtgt tgttaaggtg
    41221 atgttcttcg acgaaccttt ggatgcgtca gtgattcatg ctcctgcgga aatgcgtgtg
    41281 atgttgtcgc tgaatggcat tgctatcgca gttctcggga tcttgccaca gggtttgatg
    41341 tcggtttgtg cgtacgcgct gctgggttcc ctttaaccta aagcgtttga aagatgtgtt
    41401 tgctttaagg gttaggttaa agtgccgtct atggcggtaa tcattgctgc ggcggtgctt
    41461 gccaatctga gttttatttc aagcaggccg ttcggtgttc ttccccgtcg gacgttgaag
    41521 tcagcgtggc ttcacgtttc tgagctgggg gcggcctatt tggtatttct ttatttctgc
    41581 ctctggatgg agcgtgcgga gcacggtgtc gcttattcac aggggtggga attctacgct
    41641 gcctcgctct gtgtgtttct agtgtttggc tttcccgggt ttgtttatcg tttttttgtt
    41701 tcccggtgat gcctttcagc ttcgtttagg tttccgcagg tcgtgactgg gtgatcggcg
    41761 atcatctctt gcattagtag tgtaggtgtg tgggtaggtt cgtttttttg taaggctgcc
    41821 atcggtgcgg ccttttttat tggttgtcag ggacccattt caaaagtatt tccttgagct
    41881 ggttttgggt atagggtttg ggcaggaaat catccatgcc tgcttccaga catgtggcgc
    41941 ggtcttcgtc gagcgcattg gcggtcaccg cgatgatggg ggtgcggaga ttgttccgtt
    42001 gtcgcgcgcg aatgattcgg gtcgtttcgt atccatctaa ttcaggcatt tggcagtcca
    42061 tgagtatgat gtcgaaatcc ttcctggcga tcaggttcag aacgtctttc ccgtcgccgg
    42121 ctgtgtcgac gtgacatccg agctcttcga gctgcattgt cgccagaagc tgattgatac
    42181 gatgatcgtc ggcaatgagc acgcgtaatc cttttgttgc attcgatgtt gcagttagat
    42241 gcggttgttg tctttcgaac tgacttaaac ctacagtgat tgaatcagta cttttaactg
    42301 gcactgtgaa ccaaaatttg gaaccgtgac ggatgctgct ttctacgccg atctcacccc
    42361 cggcgagttt gacaagtttg cggcatacag ccagtcctaa acccgttccg ccgtaacgtc
    42421 gagcgtgtga gctgtctgcc tgtgtgaagg gggagaacag atgaggcatc acattctccg
    42481 ggatgccaat cccggtgtct tttacggaaa aatgtaattg tcggtgttcg ggcgcgtcat
    42541 ggcgcatcgg gtgtgcttcg agagcgacaa cgatctcgcc tgtttgggtg aatttcatgg
    42601 cgttaccgac caggttcatc aggatctggc ggagtcggtt tggatcggcg agtacgaggg
    42661 gtggtatttc gtttcggaag attggcttga gggtgattcc cttttcctgg gctttcggtt
    42721 caaagaatgc ggtgatttcc tctatcaagg actttggact gaacgggata ttttcgagct
    42781 ccatgcgtcc cgcctcgagt tttgagaagt ccagaatgtc gttgatgact ctaaggaggc
    42841 tgtcggcaga gttctgaatg tgaccaagga gccgttgctg atgtgcgtcg agcttggagt
    42901 ccgctaggag ttctgccata cccatcacgc cgttcattgg tgttctgatc tcatggctca
    42961 tggttgccag gaacagggac tttgctttgt tggcctgctc tgccgcatct ttggcgatgc
    43021 tgagctggag gtcacgggtc tggatctgtt ccagcatcgc attgaagccg ttcatgaggg
    43081 ttccgatctc atcggttccg cccggcgcca ctcggacgct gtaatcccga gtttcggtca
    43141 ccgtttgcat ttgtgctgcc agtgcctcga ccggtgctgc gatgacccgt tgcagacgcg
    43201 atgcgagcag ccatgcgatg agggtggccg ccagtaatat cccggcggcg gccaggatgc
    43261 gttcgcggac gagtttcatc agggcgctca gatcgaagaa gacttctatg cgcccgatca
    43321 ccttgtcgtt gaggcgaaca gcttgatcaa cctcaagtgt agggttatgc cataacgcgg
    43381 tcagtgtgtg ctctcggtgc gccagttcgg cttgtcctgc agtcggaatg gactggatga
    43441 ggtcagcgtg ttcggggcgt gtgctgtcga agcgcgcgaa gatcgtgccg tctggaagcc
    43501 gaaggcgcgc tgcgatgatt tcgtggttgc tggccagcgc cgacagaatt tcccgacctg
    43561 cttcggggtc gcggaaggac agggcggcgg tgctgttcaa tcccaggatg gtcgtcagtg
    43621 ataccgcgtg ttcctgtttg gtttcgatgg tggccgagaa ttcgctgctc acctgggctg
    43681 tcagtgcgac gagcagtccg attgccgtgg tgatcagcgc gatggcgatg atcttgaatt
    43741 tgatcggctg tcgcagaagg gcttcacgca tcattctgcg cctcccttgt cgtcgcccac
    43801 gacggtcgcg agattcagca gcttggcact gacgcgaagg tctgcgcggg cgatggcgtc
    43861 catgttgatg accggatgaa ttcttccgcg cttgcggatc aaggcaatca ttccgccctt
    43921 ttccgtgaag ctcggtatgt cgctgactgt gaggatcctg agcgcggttg cgcggcgaag
    43981 cagttcagga tgaaccggcc agacggcgtc gccgacgtag aggatctggc attcggccag
    44041 gtttcgcagt tcgatctgtc gggtgaccgt aaggctatgg ttatctacat tttctccctg
    44101 gacggccttg ctcaggagcg gggcgagtgt gtcgtccagt acgcagaatc tgaatgggtc
    44161 gccaggcttg cgtgcgacgt ccggccagcg gacgaagtgg acgaagttgt agaggaaggc
    44221 ggccttgact tcggcttccg caaacggctc cacgctgctc gccggcgccg tcagcgtggc
    44281 gacgcccaag gtcagcagca gtcgccaccg ccccggaaag cgggacagcg cgtgcatgat
    44341 ttctgcgagg acttggcgcg gacgcggacg gttcaaggcg ggatgccgtc agaagcggag
    44401 gttggcggtg aggtaatact cccggggaac ggggtacgcc gagctggaga tcagtgcgag
    44461 ccctccgaat tccgtgtggg cagggcccat caggttgcgg cccacgagcg ccagatcggc
    44521 cgatttgctg gcccgccacg ccaggcgcat gtccgcagtc aggtagtcgg cgacggcctg
    44581 cgcgggcaga gcgctcacgt aacgtgcctg aacatcgagt tcgaggtcat ggcgcaggtc
    44641 ggatcgggag atgatgctga tctggtggcg gggggattca ccctcggcac gcagcggcag
    44701 gagttccgta ttgccgggct tggtgtgcag gttcagatcg agatacgagt aggccgtcag
    44761 caggcgccag gtggtcgagg ggcggtaatc cagggagatt tcgaaccccg tggcgcggcc
    44821 ttccagattg ttgccggcac gtgctatcca cgacatggcg cgaggcggga aaggcgggat
    44881 cacgagcgat ggcgtgccga tcatgtccac gctgagcagc ctgtcgtagt cgttgatgaa
    44941 ggccgcggta tccaggctga ggcggggtga aagttgcgcc cggtagccga tttcgtacgc
    45001 gatgagacgt tccgaggcga gattcggatt acccacggcg ctgaccgcca cgggcagtcc
    45061 gaatgtctgg ggcgtggggg ccagactgga gttgagcgtt gcgctgtctt cgatgatgga
    45121 gggtgtccgg acggcccgtg ataccgccat ccaggccgtg ctgccggagc ctgacgtcca
    45181 ccgcaggcgg gccgacggct gccactccag gccggtgaag tcattgtgct cgcccttgac
    45241 cccgagggtc aggcgcagac gctcggccag gctgaccgta tcttcggcga agatgctgaa
    45301 caattggtcg gtgcgctgtg ggcgcgtcac gctggtgggg agggtcaggt tggcatctgc
    45361 ctggacggct cggtagccga agcccgcaat caggctatgg ggcccggcgt cggcaaaatg
    45421 atggcggaag ttcacgtcgg ccgtattgat ctgggccgtg gctgcgttgc cggccgcacg
    45481 ccagtggtcg agactgaact ggttgtagta cgcccgcaat tcgtcccggc tgccatcccc
    45541 gtgcccatgt tcccaacggc cgagcagatg ggcaccggaa tactgtcgtt cgtcgggctt
    45601 catctcacgg aaggcctcgc cctgaaccgt gaccgtgtgg cctgccgatt cccagtcgtt
    45661 gcggaagccg gcgcgtccac cgctccagcg catgtcgtcc tgggtcgacg ggtcggggtt
    45721 ggcaaaccgg tcggcctgat gcaccttggc gtacacgcgc ccgaagctgt ggtcgttcag
    45781 gcggtagccg tggcgggcga cgacttcgga ctggtggttg ccgacaaccg tgcccaccag
    45841 cgtgccttgg gtgtcttcgc tgtggcgggt gatgacgttg atcacgccat tgaccgcatt
    45901 ggccccccaa gtgctgccgc cggggccgcg gaccacctcg atacggtcga tgtcctgcat
    45961 caccagattc agttcgtccc acagcacgcc cgagaaaaag ggcgtgtaga cgctgcggcc
    46021 gtccaccatg accagcaact tgttggcaaa ctgattgttg aagccgcggg cactgacggc
    46081 ccactcggtt gccgagatac gggcgacgtt gaggccgggc accgtacgca ggagttccgg
    46141 gatcgtcgtg gcccctgagc ggcggatgtc ctcggcggtg agcacggtga ccgctgcggc
    46201 cgtgtcttcg acccgttcgc tgcgctgact ggcgatcgac acccgaatgt cggccaattc
    46261 gtcaaggctc atctgtttga ggctgctcag ggcatcggtg tcggcctgcg cgctggtgcc
    46321 caggagggcg atgcccaggg cgagggcggt gcgcgcaagg ggcgccatca ggtgatgggg
    46381 cgggcggagc ggtggcatgc ggattcctct ggcggggccg gggggcggcc ggaagttgcc
    46441 tggtcggtac ggaaaagtgc tccggattct agctgccctg cggattctgc ctgtcaccat
    46501 atgtcacgtc gggcggcata aatgaaaaac cccggccacc gcgtccacgc ggcgaaccgg
    46561 ggtgatcggg caggctgagc gatcagtcgg cgtaggcgtc catcggcacg caggcacaga
    46621 acaggttgcg gtcgccatac acatcgtcga tgcgattgac gctgggccag aacttgttct
    46681 cgccgaccca gggcagcggg aaggcggctt cgctgcggct ataggggcgg ttccagtcgc
    46741 ccaggagatc ggcctgggta tggggcgcat gcttgagggg attgtcgtcg gcgggcaggc
    46801 gaccgtcctc gacggcgcgg atctctgcac ggatgctgat catggccgca atgaagcggt
    46861 cgagttcgcc cttgtcctcg gattcggtcg gctccaccat gatcgtgccg gctaccggga
    46921 aggacatggt cggcgcgtgg aagccgtaat ccatcagacg cttggcgatg tccacctcgg
    46981 tgatgccggt ggcggccttg atcgggcgga tgtcgaggat gcattcgtgg gcgacgcggc
    47041 cctgaccgcc cgagtagagc acgggatagt gatccttcag cagggtggcc acgtagttgg
    47101 cgttgaggat ggcgatttcg gtggcctgcc gcagaccgcg gccgcccatc atggtgatgt
    47161 acatccacga gatcgggagg atgctggccg agccgaacgg ggccgcggag acggcgccct
    47221 ggccggcctg cgggcgtgcc tcgtcgcccg tgggcgccac tgcgtggtcg gccatgtagg
    47281 gcgccagatg cgccttgagg ccgatggggc ccatgcccgg cccgccgccg ccatggggga
    47341 tgcagaaggt cttgtgcagg ttcatgtggc tcacgtccgc accgatggtg gcgggggagg
    47401 tgaggccgac ctgggcgttg aggttggcgc cgtccatata cacctggccc ccgtggcgat
    47461 gcacggcggc gcagatttcg cggatgttct cctcgaacac gccgtgggtg gacgggtagg
    47521 tgatcatcag cgcggcaaga cgatcggcaa agcgggcggc cttggcctcc aggtcgggca
    47581 gatccacgtt gccgttgtcg tcgcaatcca cgacgacgac ctccaggccg cacatctgcg
    47641 cggtggccgg gttggtgccg tgggcagagc gggggatcag gcacacgttg cgctggcctt
    47701 ggccattggc cgcatggtag cggcgaatcg ccacgaggcc ggcgtattca ccctgggcgc
    47761 ccgagttggg ctgcaggcag atggcgtcga aaccggtgat ggcacgcagc tgggcggcga
    47821 ggccgtcgag gagctcccgg tagccggcgg tctgctcggc cggtgcgaag ggatggatat
    47881 cggcaaattc gggccaggtg accgggatca tctcgctggt ggcgttgagc ttcatggtgc
    47941 acgagcccag ggagatcatg gagtggtcaa gtgccaggtc gcggttctgc aggcgcttga
    48001 ggtagcgcag catctcgtgt tcggtgtggt gggtgttgaa cacggggtgg ctcaggatgg
    48061 cgtcgtcgcg cagcagaccg gtgggcagat tgccgccgcg agccgccacg gcggcgtcca
    48121 gggcggcgat gtcggccggg gcgcccaagg cgccgagcag ggcggcgacg tcggcggcgg
    48181 tggtcttttc atccaccgag atgccgaggc cgtgaccgtc caggtggcgc aggttgaatc
    48241 cggcggcctg ggcggcggcg tacagggcgt cggtgcgcgc gccggtgagg agctgaagtg
    48301 tgtcgaagaa ggcgtcggtg cgaagcgtgt acgcagcgct ccgcaggcct tcggccagca
    48361 ccgcggcgag gcggtggatg cgcgcggcga tggtgcgcag gccttgcggg ccgtggtaca
    48421 cggcgtacat cccggccatg ttggcgagca gcacctggga cgtgcagatg ttggagttgg
    48481 ccttctcgcg acggatgtgc tgctcgcggg tctgcaggct catgcgcagg gcggtcttgc
    48541 cacgggcgtc cttggacacg ccgatgatgc ggccgggcat ggcgcgcacg tgggcctggc
    48601 gggtggcaaa gaaggccgcg tggggcccgc cgaagccgag gggcacgccg aagcgctggg
    48661 ccgagccgag ggcgatgtcg gcgcccaggg cgccgggcga cttgagcatc accagggcca
    48721 tcaggtcaga ggcaaccgcg accaccgcgc ccttggcctt gaggccggcg atcgcggcgc
    48781 tcgggtcgat gacttcaccg cgttcgttgg ggtactggag gagcgcgccg aagacctcgt
    48841 gatggggggc gtcctcggcg ggcccgacga tcagctcgaa gccgaagtac tcggcgcggg
    48901 tgcgcaccac gtcaagggtt tgcggaaaga cggcttcatc cacgaagaag gcctggctct
    48961 tcaccttggc gatgcggcgc gccatggtca tggcctcggc ggcggcagtc gcttcgtcca
    49021 gcagcgaggc gttggcgagt tccaggccgg tgaggtcgat caccatctgc tggaagttga
    49081 gcagggcttc caggcggccc tgggcgatct cggcctggta gggcgtgtag gcggtgtacc
    49141 agccggggtt ctccatcaca ttgcgcagga tcaccttggg cgtcagggtg tcgtagtagc
    49201 ccatgccaat cagggatttc ttcagcacat tcttcgacgc gatggccttg agctcggcca
    49261 gggcttcatg ctcgggcacc ggctcgccga gggccagggg cgcggccagg cggatgccgg
    49321 ccggcacggt ctgggcaatc agcgtctcca cgctgtccac gcccagggcg gcgagcatgg
    49381 gcgggcgttc gaccgcacag gggccgaggt gacggtcgat gaaggcgtcc cgttgttcga
    49441 gggcggacag ggggctgctg aggaggttct gcggcatggg gaaaactcgc gtcgaaggat
    49501 cgggcgggcg cacggacgtg cgcccggagc gacaggcagt cagccgaggg tggcggcgta
    49561 gccggcggcg tcgaggaggc tgtcgaggga ggccccggcg gcgggcttga tcttgaacag
    49621 ccaggcggcg taggcgtccg cattgacgga ttcgggcgcg tccacggcgt cctggttggc
    49681 ggcgatgatc tcgccggcga ccggcgcata gatgtcggag gcggccttga ccgattcgat
    49741 cacggcgatg gcctcgccgg cctccacgct gcggccggca tccggcaatt cgaggaagac
    49801 cacgtcgccg agggcttcct gagcgtggtc ggtgatgccg accgtgaggg tgccgtccgc
    49861 ttcggtgcgg acccattcgt gggaggcggt gtagcgcagg tcgtcagggg tgttcatggt
    49921 gttcaatcct tttggggagt caaaggggag aaagggagca gggtgagatc agatcagggc
    49981 ctggccgttg cgtgcgaacg gcggcttgac cacgcgggcc ttgaggcgct tgccgcggat
    50041 gtccacctcg acggcgtcgc caggctggac acccaggggc aggcgggcca gggcgataga
    50101 tttctgcagg gtcggcgaga acgtgccgct ggtggtctcg ccgtcgccct gggcgcagaa
    50161 gacggcctgg tggctgcgca gcacgccctt gtcttccagc accaggccca gggtctggcg
    50221 gcgcgtgccc tgggcgatga gggcggcctt gccgacgaaa tcgcgggcgg cgtccttgag
    50281 gtccacggtc caggcgagac ccgcatcgag gggcgagacg ttctcgtcca tgtcgttgcc
    50341 gtaaagcgcc atgccggcct ccaggcgcag ggtgtcgcgg gcaccgaggc cgcagggtgc
    50401 cacgccggca ttgaccagat cgacccacag gccggccgct ttttcggcgg gcagggtaat
    50461 ctcgaagcca tcctcgccgg tgtagcccgt gcgggcgatg aaatactcgc cgaagtcgac
    50521 cgcctggaag ggcttgaggt gcgccgagac gacctccgcg ccgggaatgg cagcccacac
    50581 cttggcacgg gcctcaggcc cctgaacggc gatcatggcc aggtcgcggc gcacggtgag
    50641 gcgggcgtcg gcgccggaag cctgcaggcg ctcgtgcatc caggccacgt ccttgtcggc
    50701 ggtgccggcg ttgacgacga cgcggtaatg ggtgtcgctc aggtggtaga cgatcaggtc
    50761 gtcgatgacg ccgccgtccg ggttgagcat gcaggcgtac aaggccttgc ccggttcgct
    50821 gagcttggcc acatcgttgg cgatcaggcc gcgcaggaag ccccgggcgt cacgtccgtc
    50881 caggtcaagg gcgagcatgt gggagacgtc gaacatgccg gcggtgcgac gcacggcatg
    50941 gtgctcctcg atctgcgagc cgtaattgac gggcatctcc cagccggcga aatcgaccat
    51001 gcgggcgccg gcggcgacgt gggtggcgta gaggggggtg tgctggggca tggagctctc
    51061 cggtggcgaa tcggttcagt ttaggcaaac aaaagggccg gcgcgacaat gccatcgcgg
    51121 cggccccatc tgtcccttgt acctgagaga ttgcggcgtg tccgacacgc tgcgtgcccc
    51181 ttcggtggat gcggacccgg cgtggcgggc ggcatcactc tccagatttg tgattcgtcg
    51241 tcggtccttt tgcctgagcg gttccggggg ttgcgccttc ggcggttcgc agcgcgggtg
    51301 cgaacgctct cccgtacgac ggcgcgcacc ataccattgc gggcggtggg cggcaagcgt
    51361 ctgttacaat gccatggatg tggacgtcgc gaagccgctt cctgcccgtg aggggcctgc
    51421 gctatcacct gcgggagtgg gggcgcgaag gtgcgccccg gctcttcctg ctgcacggct
    51481 ggatggacgt gtccgcgtcc tttcagttcg tcgtggaagc cctgcggcac gactggcacg
    51541 tgctggcgcc ggactggcgc ggcttcgggc actccgagtg gcggggcgat gcctactggt
    51601 ttcccgatta tctcggcgat ctcgatgctt tgttgacggc cttgtcgccg gatgcaccgg
    51661 tcgatctggt cggccacagt ctgggcggca atgtggcctg cctgtacgcg ggtgtccggc
    51721 ccgcgcgggt gcggcggctg gtggcgctgg acgccttcgg ccttgccgac acggcgccgg
    51781 agcaggcgcc cggccgactg gaaaaatggc tgcgggaatt gtccgggcca gcggccttcc
    51841 gctgctatcc ggactgcgcg gcgctggccg agcgcttgcg ggcggacaat ccgcgtctga
    51901 gcgccgagcg ggctgcgtgg cttgccgagc atctgggcga ggcggacggt gagggcggca
    51961 tccgtctggc gggcgatccg gctcaccggc gcgtgaatcc ggtgctgtac cggcgtgggg
    52021 aggcccgcgc ctgctggcag cgggtcacgg cgcccaccct gtgggtggtg ccggaagcgg
    52081 cggcgctgcg tggccgcctg ggggtcagcg acgaggcgca cgccgaggcg cgtgactgct
    52141 tccgggattt tcgcgaagtg accgtggcgg acagtggcca caacctgcat cacgaccagc
    52201 cgggggaagt ggcccggatc atcgagacgt tcctgttgtc gaaggcatca tgaacccagt
    52261 caacgccgat ctgcactgcc attccaccgc ctccgacggc accctggcgc cggcccaggt
    52321 ggtgcgccgt gcccacgcca acggggtgga cctcctcgcc ctcaccgacc atgacgaact
    52381 gcttggcctt gccgaggctg cggacgcggc ccgggacgtc ggcctgcgtt ttgtgcctgg
    52441 ggtcgaggtc tccgtttcct ggctcgacca gacggtgcat gttgttggcc tgaatatcga
    52501 tccgagccat ccggcgctgg tggccggtct gcaccaagtg cgcgaagggc gggacggtcg
    52561 agcggggcag atcgccgcgg aactcgaccg gatcggcatc catggtgccc tcgaaggcgc
    52621 cttgcgctac gccggcaatc cggccctggt cagccgggcg catttcgcac gctatcttgt
    52681 ggagatcggc atcgcacgca acgtccacga cgtcttcctc cattacctcg cgcggggcaa
    52741 acccggctat gtagagcacg tctgggcgac cctggatgag gcggtcggct ggatccgtgg
    52801 tgcgggcggt gtcgcggtga tcgcccatcc gggccgctat cgtctcggtc acgtggagat
    52861 ggatctgctc ttcgagcgct tccgcgacct gggcggcgag ggtgtggagg tctgctgcgg
    52921 cgcccacgat ggggggcagg tgctggcctt ttcgcgagtt gcccgcaagt atggtctgct
    52981 ggcgtcccgt gcctcggatt ttcatggacc ggaagacagt ccggtcgacc tcggccgggc
    53041 gccgcccctg ccgcccgatc tcacgccggt gtggacgcgc ctgcagtgag cctcctcttc
    53101 ttgcaatctg tgtcatggcc cagtttttca gtcttcatcc cgaacatccc cagccccgcc
    53161 tgatccgtca ggctgcgcag atcatgcgcg agggcggcct cgtggcgttc ccgacggatt
    53221 ccgcctacgc cctcggcggg cataccggcg ataacgaact cctctcccgc atccggcgca
    53281 tccgcggcgt ggacgagcgg catcacttca cgctgatctg tcgcgacctc tccgagatcg
    53341 ccacctacgc gcgggtggat aacgcccagt accgactgct caaggcgacc acgcccggcc
    53401 cctacacttt catcctcgaa ggtaccaagg agttgccgcg gcgggtgctc catcccaagc
    53461 gcaagaccat cggcctgcgg gtgcccgatc atcccctcgt ggccgccttg ctggccgagt
    53521 tggacgagcc gttgttgagc tccacgctgc tgttgccggg tgaggaacat cccctcaccg
    53581 acgcagagga gatccgcgac cgcctggaga aatccgtcga cctggtcatc gaggccggcc
    53641 cctgccgcgg cgaggcgacc acggtgatcg acctcacctc gggtacccct gagctggtgc
    53701 gggaagggcg cggcagcctc gcgcccttcg gtctgtgagc aggcgtccgg cagctcggcc
    53761 gcggtcgggg cgcaggctct gctagaatcg gcgcccgacg cgaatccgtc ctccatgcaa
    53821 gaactcatct cctcgctggc catctgggcc ctgcccgtgc tgttcgccat caccctgcac
    53881 gaggcggccc acggctacgc ggcgcgccat ttcggcgacc ggacggccga gctggccggg
    53941 cgcatcagcc tcaatccgct gcgtcacatc gacccggtgg gcaccttgct gatccctggc
    54001 gtgatcctcg caagcagcgc cctggtgggc ggttcgccga tgctgttcgg ctgggcgaag
    54061 ccggtgccgg tcaactttgg ccgcttgcgc caccccaagg ctgacatgct gtgggtggcg
    54121 gcggccggac cgctggccaa cctgtgcatg gcgatcgggt ggatggtggt gctcaaactg
    54181 ggcttcagcc tgcccgaaaa cgcctacacg ctgcccttgc tgaagatggc tgacgcgggc
    54241 gtgcagatca atgccgtgct gatgctcctc aacctgttgc ccctgccgcc gctggatggc
    54301 gggcgcatcg tagtgagcct gctgccggcc ggcccggcgt ggaagttcgc gcagatcgaa
    54361 ccctacggct tccctatcct gctcctgctg ctgtttaccg gtatcctcgg agacatcctg
    54421 gatcccctga tggctctttt ccgatttgcc cttgcaaccg tttttggact ttgacttccc
    54481 atgttcgctg aacgcgtcct ctccggcatg cgccccaccg gccgtctcca cctcggccac
    54541 taccacggcg tcctgaaaaa ctgggtgaag ctgcaggccg agtatccctg cctgttcttc
    54601 gtggccgact ggcacgccct caccaccgat tacgacaacc ccaagggcat cgagaacagc
    54661 gtatgggaca tggtggtgga ctggcttgcc gcgggcgtcg atccgtctca ggccaccatc
    54721 ttcatccagt cgcgggtgcc cgagcacgcc gagctgcacc tgctcatgtc catgatgacg
    54781 ccgctgggct ggctggagcg ggtgcccacc tacaaggacc agcaggaaaa gctcgtcaat
    54841 aaggatctgt ccacctacgg cttcctcggc tatccgctgc tgatgagcgc ggacatcctc
    54901 atctaccggg ccgacaaggt gcccgtgggc gaagaccagc tgccccatgt ggaattcacc
    54961 cgggagcttg cccgccgctt caaccacatg tacggccgcg agcccggttt cgaggacaag
    55021 gccagagagg ccgtcaagaa gctcggctcc aagcgcgcca agctctacga ggagttccgc
    55081 acccgcttcc agcagaacgg tgacgacgaa ggcctcgccc tcggcaaggc gctgctcgac
    55141 gaagcccaga acctctccca cggcgaccgg gagcgtctct acggctacct ggaaggcagt
    55201 ggcaagatga tcctcgtgga gccggacgcg ctgctcaccg aagcctcgcg catgcccggc
    55261 ctggacggcc agaagatgtc caagtcctac ggcaacacca tcttcctgcg ggaggaggcg
    55321 gatgccctga ccaagaagat ccgcaccatg cagaccgatc cggcgcgggt gcgacgcacc
    55381 gatccgggcg atccggaaaa gtgcccggtg tggcagttcc acgtcatcta ttccgacgcg
    55441 cccacccggg aatgggtgga ccgtggctgc aagagcgccg gcatcggctg cctggagtgc
    55501 aagcagccgg tggtcgatgc ggtgctcaag gaacaggagg ccatgcgcga gcgcgcccag
    55561 ccctacctgg acgatcccac cctggtgcgc agcatcatcg ccgacggctg tgacaaggcc
    55621 cgcaagctgg cccaggaaac catgcgcgac gtgcgcgaat ccatggggct cagctactcg
    55681 tgagcgggcg ggcggggacg acgtcgggcg accggtgtgc gctgcaaggc gcggccggtg
    55741 acgggctgcg cccttgaacg gttcgccgat ggacagcgcc tccgccaacg ccgtgctcga
    55801 cctggcgccg ccggccgaag cgccggagag cgcgattggc cgcatgtacg gccagccgat
    55861 cttcgagctg ccgcgcgatc tgtacatccc gcccgaagcg ctggaggtct tcctcgacgc
    55921 cttcgaaggc cccctcgacc tgctgctcta cctgatccgc aaggccaaca tcaacgtcct
    55981 cgacatcccc atggtgccgt tgacggccca gtacctggaa tacgtggagg cgatgcgccg
    56041 gcacaacctg gaactggccg ccgattacct gctgatggcc gcgaccctcc tcgagatcaa
    56101 gtcgcgcatg ctgctgccgc ggccgccgcg ggatgacggc gaggaggtgc tcgacccgcg
    56161 ggccgagctg gtgcggcgtc tgctggaata cgagcagatc aagctcgccg ccgcgcgggt
    56221 ggacgcattg ccccgtgccg agcgggattt cgaatgggtc ggcgtgtttg tcgccgagaa
    56281 ggtggtggag cgtctgccca ccgtcagcct ccacgacctg caactggcct ggctcaagat
    56341 cgcccgccag aaccgcctgc gccggcacca cacgatccag cgcgaggagc tgtcggtgcg
    56401 cgagcacatg agcctgatcc tgcgcaagct gagcggcgac gccttcgtcg tgttcgaatc
    56461 cctgttcgat ccggcactcg gggtggccgg cctggtggtg gtctttctcg ccaccctgga
    56521 gctggtcaag gagcgcctgg tggtggtgac ccagaacgag gccttcgcac cgatctacgt
    56581 gaaacgcagc gatgcctccg ccgcagacgc ctgacgaatt caagcgcgtg ctcgaaaccg
    56641 ccctgctggc tgccccggca ccgctgcagg tgagcgagct gcgtcggctc tttgaggacg
    56701 atccgggcgg cgacctggtg cggcgcctgc tggtcgagct ggcgggcgac tggcagggca
    56761 ggggggtcga gctggtgcag accgccagcg gctggcgctt ccagacccgg cccgagtacc
    56821 agatctacct cgaccgcctg aagaacgaac gaccgcccaa gtattcccgc gcggtgttgg
    56881 aaaccctggc catcatcgcc taccgccagc cggtgacgcg gggtgacatc gaggacatcc
    56941 gtggcgtgat ggtgtcgccc agcgtcctca agaccctcga atcgcggggc tgggtggatg
    57001 tggtcggaca ccgggacacg ccgggccgcc ccggcctgta tgccaccaca cgcaaattcc
    57061 tggaggatct cggtctgcgc agcctgaccg aattgcctcc gctgaccgaa atcgaaagga
    57121 tcatggatct tgtcgaactc ccaaaaaccg aagaaccggc cgtttaggcc caagaccggc
    57181 gcgcccgcgc ccggtggcaa gatccgcggc acatctgccg gaggtccgcc gcgcggacgt
    57241 ggccgtgggt ccgccgaggc gtcccggggc gaccaggacg aaggtgcccg ccagccccgc
    57301 agcaacgcct cggccatcgg cctcggcgac accgggggcc gcggcaactg gcgtgcccgc
    57361 aaggcgcaga gcggggcggc agcctacgga ctgcccacct ccggcgacgc gctgccgact
    57421 tcggcgacgc ctgagcgtga acagcgccgt ccgcagggcc gtgacgccgc ctacaccgag
    57481 ccccagcgcc tgcacaaggt gctcgcggca gcgggcctcg gctcccggcg cgagatcgaa
    57541 gagtggatcg tcgccggccg catctccgtc aacggtgagc ccgctgacgt gggccagaag
    57601 gtcggccccg gcgaccgggt gcgggtgaat ggcaagctca tgccgatccg cttccagccc
    57661 cgcgtcccgc gggtgttgct gtatcacaag cccgaaggcg agatcgtctc gcgggacgac
    57721 cctgagggtc gcccgaccgt gttcgaccgt ctgcccatgc tgcgcaaggg gcgctggatc
    57781 gcggtaggac ggctggactt caacacctcc ggcctgctgc tcttcgtgaa cgacggcgat
    57841 ctggccaacc gcctgatgca cccgcgctac gagctggacc gggaatacgc cgtgcgtctc
    57901 ctgggcgaac tggacgaagt gcagaagcag gcgctgctgg acggcatcga gctggacgac
    57961 gggccggcgc gcttcaacac cctgctcgat gcgggtggcg aaggggtcaa tcactggtac
    58021 cgggtgagcc tgtcggaagg gcgcaaccgg gaagtgcggc gcatgttcga ggcgctcggt
    58081 ctgaccgtga gccgcctgat gcgggtgcgt tacggtccca tcagtctgcc gacgcgcctc
    58141 aagcgcggca tgtgggaaga catgcccgag gaagacgtct gcgccctggc cggtttgccc
    58201 aagccgatcc gcgcgccggg gccccgcaag ccgccccgca gcacgccgcg gcgtaccccg
    58261 ccaaaaggct gaacgggccc gaaattgcgc ctgcggcggc gggctggtat actcttcgtg
    58321 tttcaccggc ccgaaaagca gtatcaaggg ccggacatcc ggtcgaattc aacagggatg
    58381 ggcgtttcgc ccatttttca tttgtgggct aggaatttgt gggccaaggc atggatctgc
    58441 aacaactgat cgaacagacg gcggaagggc tcggctacga agtggtggac gtggaatcgt
    58501 ccccccgtgg gcgcctgctg cgcgtgttca tcgacgcgcc ggacggtatc acggtcgacg
    58561 attgcgcgac ggtcagcaat cagctctccc gcgtgttcga ggtggagaac gtggattacg
    58621 accggctgga agtatcgtcc cccggtctcg accgcgtgct caagaaggcg acggatttcg
    58681 aacgcttcgc cggcgagacg atccaggtcc gcctgaacct gcccatcgac aaccagcgca
    58741 atttcaccgg caccctggtc ggactcacgg acggtgtggt gacgctggaa acggaaaagg
    58801 gatccctcgc gatcccgttc gaagagatca acaaggcccg cctggtgccc aggttttaag
    58861 gaatttggag gtttgccggt atgagccgtg aaattttgct gttggttgac gccctggccc
    58921 gcgagaagaa cgtgtccaag gagatcgtct tctccgcact ggagtccgcc ctggcccagg
    58981 ccaccaagaa gcgcatccac gacgaagccg atgtgcgcgt gtccatcgac cgggaaaccg
    59041 gcgactatga atccttccgt cgctggcagg tgctgcccga cgaggaagtg gacaacgacg
    59101 aagcccagat ggggctgatc gatgcccgcg aggaataccc cgagatccag gtgggcgatt
    59161 tcgtcgagga atccctcgag cccatcgact tcggccgcat cggcgcccag gctgccaagc
    59221 aggtgatcct ccagaaggtg cgtgacgcgg agcgcgagca gatcctcaac gacttcctcg
    59281 agcgcaagga attcatcatc tccggcagca tcaagcgcat ggagcggggc aatgcgatca
    59341 tcgaagtggg ccgccttgaa gcggtgctgc cccgcgacca gatgatcccc aaggaaaacc
    59401 tgcgcgtcgg cgaccgggtc aaggcctacc tgctgcgcat cgaccgcggt gcccgcggcc
    59461 cccagctgat cctgtcgcgt accgcgccgg agttcgtcat ccgcctgttc gagctggaag
    59521 tgcccgagat cgacgacggt ttgcttgaaa tcaaggcctg tgcgcgcgac cccggtctgc
    59581 gcgccaagat cggcgtaaag tccaacgatc cgcgcatcga cccgatcggt acctgcgtcg
    59641 gcctgcgggg ttcccgcgtc accgccgtgc gcaacgagat cggcggcgag aacatcgaca
    59701 tcgtgctgtg gtctcccgag cccgcccagt tcgtcattgg cgccctgcag ccggcggaag
    59761 tgtcgtccat tgtcgtggac gaggaagccc atgcgatgga tgtggtggtg gacgagaaca
    59821 acctggccat cgccatcggc cgcaacggcc agaacgtgaa gctcgcgtcc gagctgaccg
    59881 gctggaccat caacctgatg accgaggccg agtcggagcg caagtccgag gaagagcaca
    59941 gttccgtgcg ccagatcttc atgagcaagc tggatgtgga cgaggaagtc gccaacatcc
    60001 tcatcgacga gggcttctcg tccctggagg agatcgccta cgtgcccctg gctgagatgc
    60061 tggagatcga atccttcgac gaggaaacgg tgaacgagct gcgcaaccgc gcgcgcaacg
    60121 tgctcctcac cgaagccatc gtcaccgagg agcagctcga gaccgtgtcc gaggatctgc
    60181 tcagcctgga tggcatggac aagccgctcg ccgccaagct ggcccaggct ggcgtccata
    60241 accgcgacga cctggccgat ctggcggtag acgagttgat ggacatgtcg ggcctcgacg
    60301 aagagcgggc caagaatctg attaccaggg cgcgcgcgca ctggttcgag taatacccgg
    60361 agggcaagat gggacagaca agcgttacgc aattcgccgg cgaactgaaa atgccggcct
    60421 ccgtgctgct cgaacagttg cagaaggcag gtgtggacaa gtcgcgggaa accgatctgc
    60481 tgaccgagca ggacaagacc cgtctgctgg atttcctgcg ccgcgcccat ggtgattccg
    60541 ccgcaaaggc caagatcacc ctgacgcgca agcagaccag cgagatcaag gcgaccgact
    60601 ccaccggccg tgcccgcacg gtgcaggtcg aagtgcgcaa gaagcgtgtc ttcgtcaagc
    60661 gtgacgaatt gctggtgccc gaagctgcgg cgccgggcgc cgcgctcgtt cccgaaatgc
    60721 ctgccgagtt gcccgaaccc gtgctcgagc cgactccggt tcccgaaccc atcgagatgg
    60781 ctgcgcccga acctgtccag gctgcgccgg tcgaggtggt cgtcgaagtc gaacccgagc
    60841 ccgttgtgga gccggtcgca cccgaagaaa cggtcgcccc cgagcctgag cctgaaccgg
    60901 tcgccgtcgt gcccgaaccc gtcgccgtgg cacccgcggc caagcctgag tcccgtcccc
    60961 agcaagggcc ggtgcgtcgc gtcgtgagtt ccgtgatctc tgccgaagag cgcgcgtccc
    61021 gcgagcgcga ggcgcagcgt caggctgagc tggccgcccg tcaggctgcc gatctcaagg
    61081 aaaagcagga acgcgaagcc cgcgcccgtg cagcgatgct gcagcgcatg gaagacgaga
    61141 agcagcggca gtccgaaggc gacaagagtc gtcaggatgc cgaggcgggt gccaagagtg
    61201 gcacgcttca caagcccgcc aaggccgatg acaagggtgc ccgcaaggac gtcaagaagg
    61261 gcacgccggt cgccaaggaa gcagccggcg acgcaaagcg tcgcggcggt ctgaagactc
    61321 gcggcgatgc agcgggcggt gcgggcggct ggaagggtgc ccgcggtggt cgttccggcg
    61381 gacgtcacca tgaggcgccg gccttcgtcg cgccgaccga acccatcatc cgtgaggtgc
    61441 acgtgccgga gacgatctcc gttgccgatc tggcccacaa gatggcggtg aaggccaccg
    61501 aagtcatcaa gaccctcatg aagatgggat ccatggtcac catcaaccag gtgctggacc
    61561 aggacacggc catgatcgtg gtcgaggaga tgggccacaa ggcgcttgcc gccaagctgg
    61621 acgatccgga cgccttcctc gaggaaaatg ccgaacacaa ggacgtggtg gaagagtccc
    61681 gtgctccggt ggtgaccgtg atgggtcacg tggatcacgg caagacctcg ctgctggact
    61741 acatccgtcg cgccaaggtg gcggcgggtg aagcgggtgg catcacccag cacatcggcg
    61801 cgtatcacgt ggataccgat cgtggcacgg tcaccttcct cgacacgccg ggtcacgagg
    61861 ccttcacggc gatgcgtgcc cgcggtgcca aggccaccga cctcgtcatc ctggtggtgg
    61921 cggccgacga cggcgtgatg ccccagacca aggaagccat ccaccacgcc aaggcggccg
    61981 gcgtgccgct ggtggtggcg gtcaacaaga tcgacaagcc gggcgcgaac cccgagcggg
    62041 tgcgtcagga gctcatcgcc gaaggcgtgg tgccggaagc ctatggtggc gacaccatgt
    62101 tcatcgaggt gtccgccaag accggtcagg gcatcgacga tctgctcgag gccatcctgc
    62161 tccaggccga agtgctggaa ctcaaggcgc cccacgacac catggccaag ggcctgatca
    62221 tcgaagcgcg cctcgacaag ggccgtggcc cggtggcgac cctgctggtc cagtccggca
    62281 ccctcaagcg tggcgacgtg atcctcgccg gcgccacctt cggccgtgtc cgcgccatgc
    62341 tcgacgagaa cggcaagccg gtggaagaag ccggtccgtc gattccggtg gaaatccttg
    62401 gtctgtccga agtgccgggc gccggggacg aggtcgtggc gctggccgac gagcgcaagg
    62461 cgcgggaaat cgccctgttc cgtcagggca agttccggga cgtcaagctc gccaagcagc
    62521 aggccgccaa gctggagagc atgttcgagc agatgggcga gggcgaggtc aagtccctac
    62581 ccctcattat caaggccgac gtgcagggtt cccaggaagc cctggcccat gccctggtca
    62641 agctctccaa cgaggaagtc cgtgtgcagg tcatccacgg tggcgtgggc gcgatcagcg
    62701 aatccgacgt caacctggcc caggcttccg gcgcagtggt catcggcttc aatatccggg
    62761 ccgatgccaa cgcccgcaag ctggccgaga acttcggcgt ggacatccgc tactacaaca
    62821 tcatctacga cgccgtggat gaggtgaaga gcgccctgtc cggcatgctg gcccccgaga
    62881 agcgcgagca ggtgctgggc atggtggaga tccgtcaggt ctttaccatc tccaaggtcg
    62941 gaaccattgc cgggtgttac gtgctggaag gtctggtcaa gcggaatgcc caggttcgcg
    63001 tgctgcgcaa ccacgtggtc atccataccg gggagctgga atccctcaag cgcttcaagg
    63061 acgatgtcaa ggaagtcaag gccggctatg agtgcggtct gcagatccgc aatttcaacg
    63121 atctcgtgga aggcgatcag ctcgaaatct acgagatcca ggaagtggcc cggaccctgt
    63181 aagcgatgcc caaggagttt tcccgtggcc agcgtgtggc ggagcagatc cgtcgcgaac
    63241 tggccgagct catccgcctg gaagtgaagg acccccgcgt cggcttcatc accctgaccg
    63301 acgtggagct caccccggac tacgcccacg caaaggtgta tttcacgtcc atgaccggtc
    63361 aggatgggct cgacgacatc ctgcgcgggc tgcgccgggc gagcggcttc ctgcgccgcg
    63421 aactggggcg ccgggtgcgc atccacaccc tgccggagct gcacttccag tacgacccct
    63481 cggtggagaa cggcaaccgc ctgtcgtccc tcatcgaccg ggccctggag tcggatcggc
    63541 agttccatcc cgaggatgaa gccgccggcg acgacacgtc tggcaagggc ccgtccgtcg
    63601 attgattgcg gggcggcgcg gccgcccgct gcgaaggttt tcccgtggtc caagctcctc
    63661 cgccacgccg tgtcccgcgc cggatcgtgg atggcgtcct gtttctcgac aagcctcagg
    63721 ggctcagttc caaccatgcc ctgcagcagg cgcggcgtct gctcaatgcg gccaagggtg
    63781 gacacaccgg cacgctggat ccgatggcga cgggcctgct gcccctgacc ttcggcgaag
    63841 cgaccaagtt ctcccagacc ctgctggacg cggacaaggc ctacgacgcc accgtcctcc
    63901 tcggcgtcac cacgaccacc gccgatgccg aaggcgaggt gctcgacaca cgcccggtgc
    63961 acgtatccga cgcgcagctg gccgcggccc tggcgcagtt gcggggcgaa atcgagcagg
    64021 tgccgcccat gtattccgcc ctcaagcacc acggcaaggc cctctacgaa tacgcccgcg
    64081 aaggggtgga gattccccgc gcgccgcgcc gggtgacgat ccatcgcctt gagttgctgg
    64141 aacgtgcggg tgacgccctg cgcatccgcg tggactgcag caaaggcacc tatatccgca
    64201 ccctggcatc cgatctgggt gccctgctgg gctgcggcgc ccacctgacc gccctgcggc
    64261 ggacccgcat cgggccgttt gcattggatg ccgcgattcc gctggctgat ttcgacgcgt
    64321 gtgcaccggc ggcacgggat gcggccctgg ctccggtcga tgcactgttg gtccacatgc
    64381 cgtgtgtcag cctggacgcc gatcaggccc gcagcctgac ccacgggcag gcggcgcggg
    64441 gttgcgcggg ggaggctggg caagcttatc gggtctacca cgtggggcgc ttcctggggg
    64501 tggctaccct cgcggaaggc gggctgctgg tcccgacgcg tctggtatcc accgcggatc
    64561 aatgaaaagc cttgagcttc agtgggatcg ttcgctataa ttccgcgctt cgcaaggcta
    64621 aactaaccga gttcattcat ggcaatctcc accgaacaaa aagcgcaaat cgttcgcgac
    64681 caccaacgcg ctcagggcga caccggctcc cccgaagtcc aggtcgccct gctgaccgcc
    64741 cgcatcaacg atctgaccgg tcacttcaag gctcacgtca aggatcacca ctcccgccgc
    64801 ggcctgctgc agatggtcag ccagcgtcgc aagctcctcg actacctgaa gcgcaagaac
    64861 gccgacggct accgtgagct gatcgctcgc ctcggtctgc gcaagtaagc agaaaaagca
    64921 gctcattgcg aagaacgtga atttgtcctg cagcaacgtg cgcaggcttc ttcatgggca
    64981 tggccggcgc aagtctggtg acttggccgg ctttgtcaca tctgtgcgct ggaaattcag
    65041 cgcgtgacct cggtcgatga agcggttgtg gtctgccgcc gaccgattga atgaaaggac
    65101 atcccttgcc tacttccatc aagaagactt ttacttacgg ttcccacacg gtcaccttcg
    65161 agaccggcga gatcgctcgt caagcgcacg gtgcggtgat ggtgacgatg gacgacacgg
    65221 tcgtcctggc caccgtggtg gcggccaaga acgccaagcc cggtcaggat ttcttcccgc
    65281 tcaccgtcga ctacgtcgag aagttctacg ccgcggggcg catccccggc ggtttcttca
    65341 agcgtgaagg gcgtcccacc gaaaaggaaa ccctcacctc ccgcctgatc gaccgcccga
    65401 tccgtccgct cttcccggat ggtttctaca acgaagtgca ggtcatcgtg caggccatgt
    65461 ccctcaatcc cgaagtggat gcggacatcc ccgccatgct cggcgcctcg gcggccctgg
    65521 ccatcgccgg catccccttc gcgggcccga tcggtgcctg tcgcgtgggc tacgccaacg
    65581 gcgaatacat cctcaacccc accgccaccc agctcaagac cagccagatg gatctggtgg
    65641 tggcggggac cgaaaccgcc gtgctgatgg tggaatccga ggcccgcgag ctgcccgaag
    65701 acgtgatgct gggtggtgtg gtgttcggcc acacccagat gcaggccgcc atcaccgcca
    65761 tcaatgaact ggtggaagtc gccggcaagc ccgaatggga ctggcagccg cccgccaagg
    65821 acgaggccct gatcgcccgc gtcaccgaga tcgctggcga gaagatcgat gctgcattcc
    65881 gcattaccgc caagcaggcc cgctcccagc agctgtccgc catcggtgat gaagtgcacg
    65941 ccgccctcac cgcagaaggc cgcgcctacg acgccaacac cgtcaacaac atcctcttcg
    66001 acctggagtc gaaggtcgtc cgcggtcgca tcctcagcgg tgaggcgcgc atcgacgggc
    66061 gcgacacgcg caccgtgcgc cccatcgaca tccgcgtcgg cgtgctgccg cgcacccacg
    66121 gttccgccct gtttacccgt ggcgagaccc aggccctggt ggtgaccacc ctgggcaccg
    66181 gccgcgacga gcagatcatc gacgcggtgg ccggcgaata ccgcgagcgc ttcctgctgc
    66241 actacaactt cccgccgttc tccaccggtg aatgcggccg tttcggctct cccaagcgtc
    66301 gtgagatcgg tcatggccgt ctcgccaagc gcgctctggc cgccgtcctg cctccgaagg
    66361 aagaattcac ctacaccgtg cgtgtggtct ccgagatcac cgaatccaac ggctccagtt
    66421 ccatggcctc ggtctgtggc ggctccctgt ccatgatgga cgcgggcgtg cccctgtccg
    66481 cgccggtggc cggcatcgcc atgggtctca tcaaggacgg tggccgcttc gccgtgctga
    66541 ccgacatcct gggtgacgaa gaccatctgg gcgacatgga cttcaaggtg gccggtaccg
    66601 acaagggcgt caccgccctg cagatggaca tcaagatcct cggcatcacc aaggagatca
    66661 tgcaggtcgc cctggcgcag gccaaggaag gccgcctgca catcctcggc ctgatgaagg
    66721 atgccctggg cgaggcccgt ggcgaggtgt ccaccttcgc gccgcgcatg ctcaccatca
    66781 agatcaaccc cgaaaagatc cgcgatgtga tcggcaaggg cggtgcggtg atccgtgcgc
    66841 tgcaggaaga gaccggcacc atcatcgaga tcaccgacga cggcaacgtg accatctcct
    66901 ccgtcagcgc cgaaggggcc caggaagcca agaagcgcat cgaggacatc accgccgaag
    66961 tggaagtggg caaggtctac gaaggtccgg tggtgcgcat cctcgacttc ggcgcgatca
    67021 tcaacatcct gccgggtcgt gatggcctgc tgcacgtgtc ccagatcgcc aacgagcgcg
    67081 tcaatgcggt cagcgactac ctcaaggaag gtcaggtcgt ccgcgtcaag gtgctcgaaa
    67141 ccgacgaaaa gggccgcgtc cgcctgtcca tgaaggcgtt gctgaacgag caggctgccc
    67201 agtaagtctc aacgggatcg tctgcacgga aaacgggcgc tgcggcgccc gttttcatgg
    67261 gttcaagccc atgcgtggcc ggcgccgtcg cgctaatatc cagccttccg caccgtcggt
    67321 gccgtctctc cgtccgctcc gctgcccatg aaatcgctca aggatcccct gctgttccgc
    67381 acccaggcct tcatcaatgg tgtctggacc gacgccgatt ccggtgcccg cctcgaggtc
    67441 tgcaatccgt tcgacggaaa ttcgctgggc agtgtccccg ccatgggggc gacggaaacg
    67501 gcgggcgcca tcgcggcggc caatgcggca ttgccggcgt ggcgcagcct gctcgcgcgg
    67561 gagcgcgccg cgatcctgca gcgctggttc cagctgatcc acacccacgc cgacgatctg
    67621 gcgcggatga tgaccctgga gcagggcaag cccctcgccg aggcgcgggg cgaggtggtc
    67681 tatgccgcat ccttcgtgga gtggttcgcc gaggaggcca agcgactgta cggtgagctc
    67741 attcccacca cgggggccga tcgacgcctg atggtgatcc gccagcccgt gggcgtctgt
    67801 gcggcaatca cgccctggaa ttttccggcg gcaatgatca ctcgcaaggt ggcgccggcg
    67861 ctggcggccg gttgcacggt gatcgtcaag cccgccgaac agacgccctt cacggccctc
    67921 gcgctggccg aactggccca tcgcgcggga tttccgcggg gggtgttcaa cgtgctcacg
    67981 ggtgatccgg tggccatcgg cggcgcgctg acggccagtg aggtggtgcg caagctgtcc
    68041 ttcaccggct ccaccgaggt cgggcggcta ctgatggccc agtgtgcgcc cacggtgaag
    68101 aagctgtccc ttgagctggg tggcaatgcg cccttcatcg tctttgacga tgcggatctg
    68161 gatgcggcgg tggccggcgc gctggtctcc aaataccgca acgccggtca gacctgcgtg
    68221 tgcgccaacc gcttcctggt gcaggatggc atctacgacg ccttcgccgc ctgtcttgcc
    68281 gaggccgtca agggtttttc ggtgggcaac gggctggacg cgggtgtgac gatcggtccg
    68341 ctgatcgatg ccgccgcggt ggacaaggtc gaggcccacg tggcggacgc cctcgcccag
    68401 ggcgcgcggg tgctcgtcgg cgggcggcgt catgcgcgtg gcggcacctt ctacgaaccc
    68461 actgtgctgg ccgacgtcac gccggccatg cgcgtggcgc gggaggaaac cttcgggccg
    68521 gtggcgcccc tgttccgctt caagaccgaa gcggaggcga tccacatggc caacgacacc
    68581 gaattcggac tcgccgccta cttctacgcc cgtgacgtgg ggcgcatctt ccgggtgggg
    68641 gaggcgctgg agtacggcat ggtgggcatc aataccgggc tgatctccaa tgaggtggca
    68701 cccttcggcg gcatgaagca gtctggcgtg gggcgcgaag gctcgcgcca tggcatcgac
    68761 gaatacctgg aactgaaata cctgtgcctg ggcgggctgg accgatgatg cgccacgccg
    68821 cgtcgctgac cccgtctgcg tgggtcaccc gctttgcacc cctgatcgcg gccgggggcg
    68881 aggtgctgga tctggcgtgt ggcagcggcc ggcatgcccg ctggctggcc gggcggggtt
    68941 accgggtcga ggcggtggac cgggatgcgg tggcgctgga attgctcgcc acggtgcccc
    69001 acgtgacgac ccgcgaggcg gacctggagg cgggcgactg gccttacgga gatcgtcgct
    69061 tcgacggcgt ggtggtgacg aactacctgt tccggccgcg cttcaatctc ttgctggcag
    69121 cgctcaaccc cggcggggtg ctgctctacg agaccttcat gctgggcaac gagcgtttcg
    69181 gcaagccgag caatccggaa ttcctgctgc gctcccacga attgatcgag cgggtggccg
    69241 gtgcgtgcac gatcctggcc tacgagcagg gcgaggtgag cgagccgaag ccggcggtga
    69301 tccagcgcat ctgtgccgtg aaggggcaca acccggtggt gcgtctgccc tgaaggccgc
    69361 cgcgctgaac ttcagtgcag caggggcttg agttccttcc agacggtttc caggatccgc
    69421 ggctgggcgc tggccaccgg gtgaagaccg tccggctgga agaaggccgg ctggtcggca
    69481 aaaccatcca tcaaaaaggg cacgaaacgc agcgtgttgg tgcgggcgag atcgcgaaag
    69541 gccttttcgt agcgggccgt gaagtccggt ccgaaattgg gcggcatgcg catgcctacc
    69601 agcaggactt gcgcgccggc cttgcgggcg gcgtcgatca tggcctgcag gttggcgacc
    69661 atggccggga cgggcagtcc gcgcaggccg tcgttggcgc ccagttcgat gatcacgacg
    69721 gccggccgct gacgatccag ggctgcgggc aggcgggccc ggccaccggc ggtggtctcg
    69781 ccgctgatgc tgccgttgac gaccgtccag gccgattttt cgcccgccag ccgctggccc
    69841 agcagatcag gccacgcttc ctggggacgc agcccgtagc cggccgagag gctgtcgccc
    69901 cacaccagga tcttgccggc gaaagcgggc gcgccagccg tcaggagcag gacggccagg
    69961 gcggcgcggc ggagcagggc agcggcgcgc acgacgggat caggcggtga gctggccttc
    70021 gcagtgcccc tggatcagtt gcaggagctg cgggaagacc ttgggtgtgc cggccacgac
    70081 gttgccggtg gtgagatagg tggactcgcc cgcaaggtcg ctgaccagcc cgccagcctc
    70141 cgagatgagc aggctgccgg ccgccatgtc ccagggcgcc aggccgaatt cccagaaggc
    70201 atccagacgc ccggcggcca cgtaggccag atccagcgcc gcggcgccag gccgacgcag
    70261 gccggcactc ttctgggtga gttccttgaa gatcgccagg taggtgtcca cgtgggcgaa
    70321 ctggcggtag gggaaacccg tgccgaccag ggcgtcctgc aggcgcaggc gcttcgagac
    70381 gcggatgcgc cggtcgttga ggaaagcgcc gccgcccttg gtggcggtga acagctcatt
    70441 gcgggtggga tcgaagacga ccgcctgttc caccacaccg cggcgggcga gggcaatgga
    70501 cacggcgtat tgcggaaagc cgtggatgaa gttggtcgtg ccatcgagcg ggtcgatgat
    70561 ccactggtac tcggtgtcct cggccgggcc ggtctcgccg gactcttctg ctagaatggc
    70621 gtggctcgga taagcctcgc gcagtacgtc gatgatggcc gattcggcgg cccggtcgac
    70681 ctcggtgacg aaatcgttgg gctgtttggt ctcgatgcgc aacaggtcca cgtcgaggga
    70741 agcccggttg atgacggtgg cggcgcggcg agcggccttg atcgcgatgt tcagtgtggg
    70801 atgcatgggc gtcctggagg tccgccggcg caggcatcgg gtgccggtcc ggcttcttac
    70861 attcggaaaa ccctgcattt taatatgaac cgtgaaggcg cgcttgggcg cgtgcgtatc
    70921 gttctttccc gcaccagtca tcccggcaat atcggtgcgg cggcccgggc gatgaagacc
    70981 atggggctgt cgcagctggt gctcgtccgt ccccagtcct ttcccgatcc tgtggccacc
    71041 gcccgtgcgg cgggcgccga cgacatcctt gcggcggcgc gggtggtcga cacccttgaa
    71101 gaggccttgc agggcaccgt gttttcggcg gccctcacgg cgcggcggcg tgagctggcg
    71161 gtgcccatgc gctgggcccg ggaggcggcc ggcgaactcg tctgcgcggc ccatggcggc
    71221 gacgtggccc tggtgttcgg caacgagacc gtggggctgt ccaacgagga agtgtccctg
    71281 tgccagatgc cggtgcgaat ccccacggcg gccgaatatg gctcgctgaa cctcggggcc
    71341 gccgtgcagg tgcttgccta cgagttgcgc atggccgcgc tggagcccgg tgagccgccc
    71401 ggcaccgcat tcgagccggc cacccacgag gagatcgagg gcttctacgc ccacctggac
    71461 cgggcgatca ccgccagcgg cttcttcaac ccggtcaatt ccaagcgtct gtggccacgc
    71521 ctgcggcggc tgtacggtcg catccggctg gaacgggatg aggtgaacat cctgcgcggg
    71581 atgttgacgg cgttcgaaaa gaagtagact aaaattctcg gaaatatagc ccgtcaccga
    71641 acgggtgatc catccattcc ctgatctggt tcgaggagat tccctgcatg ttccgtcgcc
    71701 tgcgcgaaga cgtggccaat gtcctggagc gggatcccgc ggcgcggtcc cgctgggagg
    71761 tgctgacctg ctatcccggc atgcacgcgg tgctgctcca tcgggtggct cacgcggcat
    71821 gggggcgggg cttgcactgg gtgggacgct tcgtcagtca tctcgggcgc atgctgaccg
    71881 gcatcgagat ccatccgggg gccaccatcg gccgccgggt cttcatcgat cacggcatgg
    71941 gcgtggtgat cggcgagacg gctgaagtgg gcgacgactg cacgatttat cagggcgtca
    72001 ccctgggcgg cacctccctg taccgcggta ccaagcgcca cccgaccctc ggccgaggcg
    72061 tggtggtggg cgccggcgcc aaggtgctgg gcggtttcga ggtgggggac ggcgccaaga
    72121 tcggctccaa cgccgtcgtg atcaagccgg taccggccgg ggcgacggcg gtgggcaatc
    72181 cggcgcggat cgtcgatgcg ggcaaggatg agtcgcctga cgtgaaggcc gacgcgccgg
    72241 cgcgccaggc cttcacggcc tacggcgtga cgcgggatct ggacgatccg ctggtcaagg
    72301 ccttgcacgg gatgatcgac catgcggccg agaccgaccg gcgcctgcgg gtgctggtcg
    72361 agcgcctcgc caaggcgggc attccggtgg atgacgcggt gctccgggag gatggcttcg
    72421 atccacagaa gctgggcaag atggtggatt aggctggagg gccgggggcg ccgcgaaata
    72481 gttgatggtt tttgtcgggt ttgctattct tgactgaaat attcaactat tcccctacca
    72541 gcccccggga gtgacgatga gactgaccac caaaggtcgt tttgcagtga cagccatgat
    72601 cgatctggcc ctgcggtgtg ccgacggacc cgtgacgctc gccggcatcg ccgagcggca
    72661 gcggatttcc ctgtcctacc tcgagcaact ctttggcaag ctgcgccgct tcgaactcgt
    72721 gtccagcgtg cgcggacccg gtggcggcta caccctggcc cagccgatgg aactggtgac
    72781 ggtcgcggac atcatcgccg cggtggacga gcctctggat gccacgtcct gcggcggcaa
    72841 ggaaaactgt ctcgacgagc atcgctgcat gacccacgat ctgtggacca acctcaaccg
    72901 gcgcatgcag gaatatctgc agtcggtgac cctggcgagt cttgttgacg cccagcggca
    72961 gaaggccctg gcggtgtccg tgctgcatga cgaacgtgcc gcaatcgccc gccacgaggc
    73021 cggccagtcg ctgacagcct gaaccgcgca ggtccgccat gtttgccccg atctacctgg
    73081 accacaacgc caccacgccc ctcgatcccg aggtgcgtga ggcgatgttg ccctggttgg
    73141 gcgggcgctt cggcaacgcg tcgagccgtc atgagtacgg ccgcgccgca cgcatggcca
    73201 tcgacgaggc gcgtgcccgg gtggcagaag cggtgaacgc ccatcccacc gaggtgatct
    73261 tcaccagcgg cgggtcggag gccaataacc tgttcctcaa gggggcggcg gcccggctca
    73321 aggcgggtgc gctggccctt ggtgccatcg agcacccctg cgtgcggcag ccggcccggc
    73381 agttgcgcca gtcgggctgg acggtgcgcg agctcgcggt ggacgcggcc ggccggatcg
    73441 acagtgcggc ctttacagcg gtgctggctg agcggccccg gctggtgtcg gtgatgctgg
    73501 ccaacaacga gaccggtgtc gttcaggacg tggcccggct ggcggcgcag gtccgtgccg
    73561 cgggtggatg gatgcatacc gatgcggttc aggccttcgg caagctcccc gtggatttcc
    73621 gggcgctggg cgtgcacggt atgtccctgt ctgcacacaa ggtgaatggt cccgtcggcg
    73681 ccggcgcgtt gattgtggac aagcgggtcg agctcgcgcc gctggtggcg gggggcggtc
    73741 aggagcgcga tctgcgctcc ggcaccgaga acgtgctggc cattgtcggt ttcggcaagg
    73801 cctgccagct ggcggcggca cgccaggcag accgggcggc ggtactgcgg ggcctgcagg
    73861 cgcggctgga tgccggattg accgggctgg gggcgacgat cttttcggcc ggcgccgagc
    73921 gcctgcccaa caccactttc tttgccctgc cgggcatcga tggcgagacc ctggtgggca
    73981 agctcgaccg ggccggtttt gcggtggcga gcggttcggc ctgctccagc gcccacccgg
    74041 agccgtcgcc cacactgctg gcgatgggcg tgacgccgga gtgtgcccgt gcggcggtgc
    74101 gcgtgagcct gggctcgggc aataccgggc aggatgtaga tggattcctg gaggcgatcg
    74161 cgcagatcgt cgaccacctc agaaacctga ccgcgatggc ggtcggagtc gcagacgcgg
    74221 cttgattacc cagtgcggag acgatgaata tgctcaagtt ccccatctac ctcgattatt
    74281 cggcgacaac ccccgtggat ccgagggtcg cggagaagat gattccttac ctcaccgagt
    74341 tgttcggcaa cccggcgtcc cgcagccacg ccttcggctg gtccgctgaa aaggccgtcg
    74401 aggaggcccg cgaacaggtg gccgcgctgg taaacgccga tccgaaggag atcatctgga
    74461 cctccggcgc caccgagtcc aacaacctgg ccatcaaggg cgcggcgcac ttttattcag
    74521 gcaagggcaa gcacctgatc accgtgaaga ccgagcacaa ggccgtcctc gacacggtgc
    74581 gcgagctgga gcgccagggc tttgaggcca cctacctgga tgtgcaggac aacggcctga
    74641 tcgacctgga ggtgctgaag gcagcgatcc gcccggatac ggtgctggtg tcggtgatgt
    74701 tcgtgaacaa cgaggtcggc gtcgttcagc ccatcgccga gatcggcgag ctgtgtcgcg
    74761 agaagggcat tgtcttccac gtggacgcgg cccaggccac cggcaaggtg gacatcgacc
    74821 tggccaggct gaaggtggat ctgatgtcct tcagcgccca caagacctat ggtcccaagg
    74881 gcatcggcgc cctctacgtg cggcgcaagc cccgtgtgcg cctggaagcg cagatgcacg
    74941 gtggtggcca cgaacgcggc ctgcgctccg gcactctggc gactcaccag atcgtcggca
    75001 tgggtgaggc cttccgcatc gcccgtgagg aaatggccca ggaaaacgcg cgcatcctgg
    75061 ccctgcgcaa ccgcttgttc gaagggctct ccgccattcc ggaaaccttc gtgaacggcg
    75121 atctggtgca ccgtgtgccc cacaacctca acatgagctt tgcctacgtg gaaggcgagt
    75181 ccctgatcat ggcgatcaag gacgtggcgg tgtcgagcgg gtcggcgtgc acgtcggcga
    75241 gcctggagcc gtcctatgtg ctgcgtgccc tcggccgcaa cgacgagctg gcccacagtt
    75301 ccatccgctt caccatcggc cgctttacga cggtcgagga aatcgactat acaattgatt
    75361 tgttgcagcg caagatcggt aagctccgcg aactgtcgcc cctgtgggag atggtccagg
    75421 agggcatcga tctgaacacc gtgcaatggg ctgcccactg acaggagaac gaagatgtct
    75481 tacagcgaaa aggttctgga ccactacgaa aacccgcgca acgtcggttc gttccagaag
    75541 gaagacgacg gtgtggggac cggcatggtc ggcgcaccgg cctgcggcga cgtgatgaag
    75601 ctgcagatca aggtgggcaa ggatggcgtc atcgaggatg ccaagttcaa gacctatggg
    75661 tgcggctcgg ccatcgcgtc gagttccctt gtgaccgaat gggtcaaggg caagaccctc
    75721 gaccaggcgc tggagatcaa gaacaccgcc atcgccgagg aactggccct gccgcccgtg
    75781 aagatccact gttccatcct ggctgaggac gctatcaagg cggcggtcga ggattacaag
    75841 aagaagcaca acggttgaaa ggaaggcaat catggcagtc accctgtccg aaaaggcagc
    75901 ccagcacatc gacaacttca tcgcgaagcg gggcaagggc atcggcatcc ggctgggggt
    75961 gcgcacctcc ggctgttccg gaatggccta caagcttgaa tttgcggatg aggtgcagtc
    76021 cgacgacgtg gtcttcgaga gccgcggcct gaaggtgctg gtggatccga agagcctgcc
    76081 ctacatggac ggcaccgagc tggactttgt gcgtgaaggc ctcaacgagg gcttcaagtt
    76141 caacaatccg aacgtgaagg accagtgcgg ctgcggcgaa agcttcacgg tctgacatga
    76201 tcgacctgcg ccaggaccac ttcgccctgt tcggccttgc gccggctttc cacatcgatc
    76261 tcgatcagct cgagcaggcc tacctgaccc ttcagggcaa ggttcacccg gaccgcttcg
    76321 cccatctgtc ggatcaggag aagcgtcagt ccatgcagtg ggccacccac gccaacgggg
    76381 ccttccgtac cctgcgccag ccgctcaccc gcgcccagta cctgctggag ctgcgcggcg
    76441 tggatccggc cttcgagaac aacacggcca tggcgccgga gttcctgatg gaacagatgg
    76501 agtggcgcga agcccttggc gaggcccgtg aagcggccga cgcgtccgcc cttgacgaca
    76561 tggcgcggcg catccgtgag cgttcccgcg cgctggtcgt ccagatcggt gacgagatcg
    76621 acacgcttgg cgatctcccg gcagcggcgg atacggtgcg gcggctgaag ttcctcgaac
    76681 gcctgcaaga tgaaatcaac gacgccctgg cgtcactgga ttcgtgatgg ccctgttaca
    76741 gatttccgag cccggtctgt ccacgctgcc gcaccagcat cgcctggcgg tgggcatcga
    76801 cctggggacg accaattccc tcgtggcaac ggtgcgcaat ggcgtgccgc aatgtctgca
    76861 cgatgacgac gggcgcgtgc tgctgccctc ggtggtccag tatttgcccg acggacgggt
    76921 tgccgtgggc cgtggggccc aaaaggccca ggccgttgat ccccgcaaca ccatcgtctc
    76981 ggtcaagcgc ttcatgggcc gtggtctcaa ggacgtggcc cacgtggaga ccatgcctta
    77041 cgacttcgtg gaccagcagg gcatggtgcg cctgcgcacg gttcaggggg tgaagagccc
    77101 ggtggaagtg tcggctgaga tcctcagcag cctgcgtctg cgcgccgaac gcagcctggg
    77161 cggcgaactc accggcgcgg tcatcacggt gccggcctat ttcgacgacg cccagcgcca
    77221 ggccaccaag gatgccgcgc gcctcgccgg gctcaacgtg ctgcgcctgc tcaacgagcc
    77281 caccgcggcg gccatcgcct acggcctgga gaacgcgtcc gaaggggtgt atgccgtata
    77341 cgatctgggc ggcggcactt tcgacatctc catcctgcgc ctgtcccgcg gcgtgttcga
    77401 ggtggtcgcc accaatggcg atgcggttct cgggggggac gatttcgacc atcgtctgtt
    77461 ctgctggatc ctcgacaacg ccggcatctc gctgccgtcc gaccaggacg cgcgccgcct
    77521 gcagatgaag gcgcgggaag tgaaggaacg cctgaccacc gacgaatcgg tagacatccg
    77581 tctgaccctc acccgtggcg atgaggtgga cctgaccctc acccgagagc agtttgtcga
    77641 gatgacccgt catctggtga gcaagaccct cgcgccggtc cgcaaggcct tgcgcgacgc
    77701 agggctcgcg cccgaggacg tgaagggtgt ggtgatggtg ggcggtgcga cacgcatgcc
    77761 ccatgtgcag aaggcggtgg gcgagtactt cggccagacg cccctcacca atctggatcc
    77821 ggacacggtc gtcgccatcg gtgcagcgat ccaggccaac gtgctggcgg gcaaccgggc
    77881 ggccgaggac gactggctgc tgctggatgt gattcccctg tctctgggca tcgagaccat
    77941 gggcggactg gtggagaaga tcattccccg caatgccacc attccgacgg cgcgggccca
    78001 ggacttcacc accttcaagg acggtcagac ggccatggcc cttcatgtgg tgcagggcga
    78061 gcgggagctc gtgagcgaat gccgctcgct ggcgcgcttc gaactgcgcg gcatcccgcc
    78121 catggtggcg ggggcggcgc gcatccgcgt gaccttccag gtggatgcgg atggcctgct
    78181 gtccgtgtcg gcgcgggaga ccctcagcgg ggtcgaggcc cgcgtggagg tgaagccctc
    78241 ctacggactg tcggacgatg agatcacgcg catgctccag tccggcttcg agtacgcccg
    78301 ggccgacatg gaggcgcgag gccttaagga gcagcaggtc gaagcggcac gggtcatcga
    78361 ggcgaccgaa aaggcgctga tgtcggatgg cgacctgttg acggcggacg agcggaccgc
    78421 cctggacgcc tcggtggagg cactgcgcag tgccgcggcc ggcctcgacg cgcgggccat
    78481 caaactggcc cttgagtcct tcaaccgggc ctccaacgag ttcgcatccc ggcgcatgga
    78541 ccggagtatc cgcgccgcct tgtccggcca caagatcgac gacatccgga tctgatccgt
    78601 tcaacgactg cattcatcat gaccacactg atcgttctgc cccacgtgga gctgtgcccg
    78661 gaaggggcgg tcatcgacgc ccgtcccggc gagtccatct gccagagcct gctggagaac
    78721 ggcatcgcca tcgagcacgc ctgcgaacag tcctgcgcct gtacgacctg ccacgtcatc
    78781 gtacgggacg gatttaacac cctggaaccg gcggaagacg aagaggagga tctcctcgac
    78841 aaggcctggg gactggagcc ccagtcgcgc ctgtcctgtc aggcgctggt gggcggtaca
    78901 ccgctgacgg tggagatccc gcgctattcc atcaacatgg tcaaggaggg caagcgggga
    78961 tgaaatggac cgacgtgcag gaaattgcca tccagttgtc tgaagcccgc ccggacgcgg
    79021 atccggcacg ggtgaacttt gttgacctga tgagctgggt catggcctta ccggaattcg
    79081 acgacgaccc gaagcgttgt ggcgaacgca tcctcgaagc gatccagcag gcgtggatcg
    79141 acgaagtcgc ctgagcatgc ggccagcgag accggcatga gcggcggcgc acagcttcac
    79201 gcctttgaac tgccggccct gcagggcggc gtgctgcgtc tggccgacta cgcgggcaag
    79261 gtgctgttga ttgtgaacac agccagccgt tgcggtttca cgccccagta cgccgggctt
    79321 caggccttgt acgaaaccta tggtgcgcag ggtctggtgg tgattgcgtg tccatgcgac
    79381 cagtttggag ggcaggaact gggcagtgcg gaggagatcg gggccttctg caccgggcgt
    79441 ttcgatgtcc gttttcccat ggcagacaag ctgcgggtca atggtcccga cgcgcatccc
    79501 ctgtttcatt acctcacggc ggccctgccg ggggtgctgg gatcccgcgc catcaagtgg
    79561 aacttcacca agttcctggt ggatcgcgtc ggaaatccgg tccggcgcta tgcgccgacc
    79621 acgccgccag ccgaactggc tggcgatatt gaagccctgc tgtaagggat cagcgtgccg
    79681 tcggatcgga cggttcgttg agggacagcc agtacaggcc cagctggccc acagcctgca
    79741 ggaaacgctc gaaatccacc ggtttgcgga tgtagctgtt ggcgccgagg cggtaggcct
    79801 cgaagatgtc ctggtgttcc ttggaggtgg tcaggacgac caccggcaac agggcagtgc
    79861 gctcgtcagc gcggatgcga cgcagcacct caaggccgtc gatgcgcggc agcttgaggt
    79921 cgagcagtac gaaggccggc atctggtggc tgtcgcgcgt tgaatactga cccgtacaga
    79981 agaggtagtc gagcgcttcg acgccatcgc gagcgaccac gacctgattg gcaatcttgt
    80041 tcttgctgaa ggccctgagg gtcagtgctt cgtcgtcggg gttgtcttcg actagcagta
    80101 tggggcgatc ggtcgccatc ttttgagctc cttgaccttg cttttcggtc tttttatgtc
    80161 gttctttaac cgctggcggt cccctttccg ggtaagtacg atcataagga gctgtccgta
    80221 gtttcagtaa ggacggagtt ggcgtttatc gggtgctttt acaacaattg acgccgattt
    80281 atcaaaaata tcttttcgtc gccactgggg gccgaaagcc aggtcggagc gagggtcggg
    80341 aaggccgctt cgaagagatc ccgattgtgt ccgacttcca cggcgagctg accgtccggc
    80401 gcgaggaaac ggggcgcatc ggcgagcagg cgacggacca ggtcgaggcc gtcctcgccg
    80461 gcgcccaggg cgagtgccgg ttcgtgccgg tattcggcag gcagatcgtc catcgcgtcc
    80521 tgggtgacat agggcgggtt gctgaggatg aagtcgaagg gctgttccgg cacctggtcg
    80581 aaggcatcgg actggaccag ggtgatgcgg tcctccaggc cgtaggcggc cacgttgcgg
    80641 gcggcaattt ccagcgcgtc cggtgaaatg tcgacggcga cgatctctgc atggggaaag
    80701 gcgtgtgcca tcaggatggc gaggcagccg gagccggtgc acatgtccat ggcacgcgtg
    80761 acactcgcag gatcctggat ccacgggctg aagccttcgt ggagcagctc ggcgaaatag
    80821 gagcggggaa tgatgacccg tgggtcgatc tcgaagcgga actcgccgag ccaggcttct
    80881 ccggtgatat aggcggccgg caggcggtcg acggcgcgct tttcgatgac ctcgaagatt
    80941 gcttcccgct catcgccggg gatgcaggcg tcgaggaagg ggtcgaggcg gtccaggggc
    81001 agggccagcg tatggaggac gagatagacg gcctcgtccc aggcgtctac gaagccgtga
    81061 ccgaagaagc agccggcgcg gttgaaacgg gtgaccgcat agcgcagcca gtcacggacg
    81121 gtgacgagct cggcgagggg gccatgctcg tgttcgtgtt cgtgctcgtc gtgcccggcg
    81181 tcgtcgggaa gggtgtcttc gtgggccatg ggtgctccgg tcaggcgagg aggtgttcga
    81241 gggtgcggcg gtagatgtcc gacagcgggt cgacggcatc cagtgcgatg cactcgtcga
    81301 tcttgtggat ggtggcgttg acagggccga actcgaccac ctgaggacag atgtcggcaa
    81361 tgaagcggcc atcggaggtc ccacccgtgg tggagagttc gggcgtgcgg ccgagggcat
    81421 cgcggatggc ggcttcgagg gcgcctacca gggtgccgcg gggcgtcagg aagggcttgc
    81481 caccgagggt ccaggcgagg tcgtagtcga gcccgtggcg gtcgaggatg gcgtggacgc
    81541 gctgcttcag ggcgtctgcg gtgtgcaccg tggcgaagcg gaagttgaag aggacctcca
    81601 gcgtgccagg aatgacattg gtcgtaccgg tgccagcgtg gatgttggac atctgccagg
    81661 aggtgggcgg aaagtattcg ttgccttcgt cccagcgggt ggcggcgagt tcggtcaggg
    81721 ccggtgcggc caggtgaatg ggattcctgg cgaggtgggg gtaggcgata tggccttgga
    81781 cgcccttgac cgtgagcttg ccggagaggc tgccgcgacg gccgttcttg atcatgtcgc
    81841 cgagcacgtc gacggacgtg ggttcgccga cgatgcagaa gtccagggtc tcaccgcgtg
    81901 ccttgagggt gttcaccacg tgcacggtgc cgtaagtggc gtcgccttcc tcgtcggagg
    81961 tgaggaggag ggcgatggag ccgggatgat gggggtggct ggcgacgaag tcttcgatgg
    82021 ccgtgacgaa ggcggcgagg gaggtcttca tgtcggcggc accgcggccg tagaggaaac
    82081 cgtcccgttc ggtgggttcg aagggatcgg actgccactg ggcgaggggg ccggtgggca
    82141 ccacgtcggt gtggccggcg aagacgacca gcgggcgggc cgtgccgcgg cgcgcccaca
    82201 ggttgcaggt accgcccgca tcgaggcgtt cgcagacaaa gcctaggggc gtgagccgtg
    82261 aggcgatcag gtcgaggcag ccggcgtcct cgggggtgac ggagcggcgg gcgatgagtt
    82321 cacgggtcag ggcgagcgtg gcgctcgccg gggctgtcgg tcgggtcatg ggtgtggggt
    82381 cggggctggg tcagtcttcg tcgagttcga gcgtgaactc cttgaacgac gcgccgcctt
    82441 cttcctgatg cggttcgctg tcgatcgacg tggacggcaa gggagtcttg tcggcgttct
    82501 tgtcgctgaa ctgggcgttg ttgatgagcc aggacaggtt ggtgggcgag tcggcgttgg
    82561 cgagggcttc ttcgagggtg atgatcttgt cccggtacag acggaagaga tcctgttcga
    82621 aggtgcgcga gccggaggcg aggctctgct ccatggcctc gcggatgccg gagatctcgc
    82681 cacgctcgat cagttcggcc acctggcggg agttgaggag gatttccacc gcggggatgc
    82741 gtttaccgtc cgggcgcttg accaggcgct gggagatgat gcacttgagg gtcgccgcca
    82801 ggtccatgta gaggagcggg cggttttcca tcggaaagaa gttgaagatc cggttgagtg
    82861 cgtggtaagc gttgttggcg tgcagcgtgg ccaggcacag gtgaccggtc tgggcgtagg
    82921 cgatggcggc ctgcatggtg tcccggtcgc ggatctcgcc gatcaggatg cagtccggcg
    82981 cctggcgcat ggcgttggac agggcgtggt tccagtccag ggtgtcggtg ccgatctcgc
    83041 gctgattgac gatggaggcc ttgtgccgga agaggtattc gatgggatcc tcgatggtga
    83101 ggatgtgccc ggagaagttc tcgttgcggt ggtccagcat cgacgcgatg gtggtggact
    83161 tgcccgagcc ggtggcgccc acgacgagca cgaggccgcg tttttccatg atcacgtcgg
    83221 acagcacggg tggcaggccg agggcgtcga ggcggggaat ctggtcgagg atgtaacgga
    83281 cgacgatggc aatgctgttc cgctgccaga agatgttgac gcggaagttg cccatgttgc
    83341 gccggctgaa ggagaggttc atctcccgtt cgttctcgaa gcgggcgatc tgctccggcg
    83401 tcatcatctc gtaggccatg cggtggatca tgcccgcgtc catggcctgc tgattgatcg
    83461 gcagggtgtt cccgtcgatc ttgatatgga tgggcgcacc caccgtgacg tagatgtccg
    83521 aggccttctt ctcggccatc agggtgaaca gcttgtcgat gatcatcggt gggcccggct
    83581 cgaatgaaac gggccgcgtg accgcggccc gggggaaggt cagtcgcgca gcagttcgtt
    83641 gatgctggtc ttggagcggg tctgggcgtc caccttcttg acgatcacgg cgcagtacag
    83701 gctgtacttg ccgtcgccag agggcaggtt gccgctcacc acgacggagc cctcgggcac
    83761 gcggccgtag agcacttcgc cagtggtgcg gtcgtaaatc ttggtgctct ggccgatgta
    83821 cacgcccatg gagatgacgc agttgtcctc cacgatgacg ccttccacca cttcggagcg
    83881 ggcgccgatg aagcagttgt cgccgatgat ggtgggattg gcctggaggg gttccagcac
    83941 gccgccgatg ccgacgccgc cggagaggtg aacgttcttg ccgatctggg cgcaggaacc
    84001 gacggtggcc caggtgtcca ccatggtgcc ttcatccacn nnnnnnnnnn nnnnnnnnnn
    84061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    84121 nnnnnnnnnn nnnnnnnnnc gttgcgcaaa ggcggcaatc cgccgggcgg cttcgaggca
    84181 ctcggccagt tcggccacca gggcgatacg gacaaagcct gcaccggggt tggtcccgtg
    84241 ggcctcgcgg gccaggaaac tgccgggcag gaccgtcaca ttatattcgg cctgcaggcg
    84301 gcgggcgaat tcggtgtccg agaggcccag cttgtccacc cgcgcccaca ggtagaagcc
    84361 cgcgtcgggc aggctcacct ccaggtgttc ggcgatgagc ggggtgacgc ggtcgaactt
    84421 ttcccggtac agacgccggt tttcggccac gtgggcttcg tcgctccagg ccgcggcgga
    84481 ggcggcctgt accaccgggc tcatggcgca gccgtggtag gtgcggtaga gcaggaagcg
    84541 cttgaggatg gtcgcgtcgc cggccacgaa gcccgagcgc atgcccggca cattggagcg
    84601 cttggacagg ctggagaaca tcaccacgtt gcggaagtcg ctgcggccca ggcgctgggc
    84661 ggcttccagg ccgccgatgg gcagggcgtc gtcgttgaag tagatctcgg agtagcactc
    84721 gtcggcggcg atgacgaagc catggcggtc cgacagctcg aacaggtgct gccattcgtc
    84781 gagggtgagg acgcggcccg tggggttgcc cggcgaacac acatacacca gttgcacgcg
    84841 ggcccatgcc tcgtcgggca cgcgggcgaa gtcggatccg aaattgtcgt ccggcaggtt
    84901 gttgatgaaa tacggctggg cgccggccag ataggcggcc ccttcgtaga tctgatagaa
    84961 cgggttggga cacagcacca gggcgtcggg ccgggtgccg tcgatgacgc actgggcgaa
    85021 ggcaaacagt gcctcgcggg agccattcac cggcagcacc tgggtggcgg gatccacggc
    85081 gggcagaccg tagcgggatt ccagccaggc ggcaatggac ttgcgcaggg cgtcggagcc
    85141 ctgcgtcgtc ggatagttgg cgagaccgcc cagatgggcc accagggcgt cgcggatgaa
    85201 ggccggcgtg gcgtgctggg gttcaccgat ggacaggcgg atcggtgagt attcggcggg
    85261 cgggacgatg ccgtcgaaga gggcgcggag cttttcgaag gggtaggggt gcagatcctg
    85321 gaggtgcggg ttcacggtcg gctggagatc ctgaagtcgg cccggatccg ggcgggaaag
    85381 gcggtgaacg gcgcggaaaa cgcgccggct ggcctcgtca agggccaatg gtattatagc
    85441 ggccacctga catgcggcag tgcagcaatg cgctgcccgc gccctcgaga aattctccgc
    85501 ggcacgccct ggcggccccc agttgacacc ctgaccacgt gcgtctcgcc aaactcaagc
    85561 tcgccggttt caagaccttc gtagacccga ccaccgtgct cacgccgggc aatctggtcg
    85621 gcgtggtcgg tcccaatggc tgcggcaaat cgaacatcat cgatgccgtg cgctgggtgc
    85681 tgggcgaatc gcgggcgtcg gcgctgcgcg gcggatcaat gctggacgtg atcttcaacg
    85741 gttccaccac ccgcaagccg gtgtcgcggg ccagcgtgga gctggtcttc gacaacgccg
    85801 aggggcgcgc ggcagggcag tggaagccct acgccgaggt ggcggtgaag cgcgtgctcg
    85861 accgcagcgg cgaatccacc tatttcatca acggcctgca ggtgcgccgc aaggacgtga
    85921 tcgacctgtt cctcggcacc ggcctggggc cacgcgccta cgccatcatc gagcagggca
    85981 tgatctcgcg gatcatcgag gcgcggccgg aggaggtgcg cggcttcctg gaagaggccg
    86041 ccggcgtcac caagtaccgg gaacgccgca aggagacgga aggccgtctc accgacgccc
    86101 gcgacaatct ggcgcgcctg gaggacatcc gccacgagct cggtcagcag atcggcaggc
    86161 tagaggccca ggccgcggtg gccgagcgct accaccggct caacgcaagc ctgtcggagc
    86221 gccagaatct cctgtggctg atcaagcgca acgaggcccg cagcgagcgg acccgcctga
    86281 cggaggaact cggtgaggcc acgcgccgga tcgaggccga ctcggcccgc ctgcagacgc
    86341 tggaaagcca gctggaaagc gcccgcgagg cccagtttgc tgcctccggt cacgtccagc
    86401 acgcccagga tgccctgcat ggggtcagta ccgaactgac ccgcctggag ggcgaactgg
    86461 cccgcctgcg ggattcccgg cgcgtgctgg agagccgtct ggtgcagttg gagcgggatg
    86521 acgcccagtg gcgcagccag ctcgacaccc tgagcagtga cgccgaacgc tggcacagcc
    86581 tgcaggaaca cgcggccctg cgtgccgagc aggccgaggc ccgtcatgcc gaactcgccg
    86641 agcgtctgcc cgaagccgag gacgccctgc gcaccgccga tgccaccgcg gcggcggtgc
    86701 gccgtgagct gaaccaggcg gaacagcaga tccgtgtcga ggaggcccac cgggtcagca
    86761 gcgagcggac cctcgagacc ctcggccagc gacgcggtcg cctgcaacag gagcagtccg
    86821 cggtggatgc gccggatctg cgccgaatcg ccgaacggga ggccctgctg tcggagctcg
    86881 aagataccct ggccacccgc cagagtgccg tggccgacgc ccaggccggg ctgcccggcg
    86941 cccagcaggc cctgcgggag gccatggaag ccgagcgcca gactcagcgg caggccaccg
    87001 aactgcgtgc ccgccgcgag gccctggtgc agctccagca caaggtccag gcccagggcc
    87061 agttgggcga ctggctgcgc cgccatcgcc tcgacggcct cacgcccctg tggcagcggc
    87121 tgcaggtggc ggccggctgg gaatcggcgg tggaggccat cctgcgggaa cgcctcgcgg
    87181 ccctgccggc cgaggacggg cgcgcggcgc tgattgcgtt ggccgacaag gtgcccggcg
    87241 cgttcgcctt cctgctggcc gatgcgccgg ctgatggggc gccgcccatg gcgctggaag
    87301 gcgcgcggcc cctgctggat catgtctgtg ccgatgtgac gctgacgccg gtgctggcag
    87361 cctggctggg cacggtgctg gccgtggacg atctggcgcc ctggctggaa cggcgcgccg
    87421 aactgtcgcc ttccgtccgg ctggtggcca aggacggtcg cgtgctggac cgccacgccc
    87481 tgatccacta tgcgccggac gcccgtaccc acggggtgat cgaacgccag cgggagatcg
    87541 accagctcgc cgtcgagctc gaccgggccc aggaggctgc ggcgcaggcc aaggaggccg
    87601 tggcggaggc tgacgccacc gcgacgcgcc tgcaggacgg cctcaccacc ctgcgtcgcg
    87661 acgtgcaggc cctgcaggcc caggtccatg gcgaacaggt ggaggtgctc aaactcaacc
    87721 aggcgcgggt ccgttacgag gaacgctggg cccagatcga acgggacatg ggcgagatcg
    87781 ccttgctgga gcagaccgag cgcgaccgcc tgatgcgctc ggagaccgag ctggaacgca
    87841 gccgcgagat ggccgagctg caacgcggac gcctcgacgc cgccgaagcg gtgcgccgcg
    87901 agcgggaggc agccctgcgc gagacccgcg cgctggagca ggccctggcc cgcgagctgc
    87961 aggacgcccg ccacgcagaa cgggaatgca ccagcaagct ggacgacatc gcccgcaacc
    88021 gtagcctggc ggaagagcag ctggcgcgca ccgccgaaga gctggaagcg cgccgcgaaa
    88081 gcctgctgga gatcgacgag ccctcggtgg ccgcggcgct ccaggcggcg gtggtcctgc
    88141 gcggcacacg ggagatggcg ctggtcgagg tgcgcaacgg gctggaggcg atccacgccc
    88201 aggtgcgcgg cctggaggaa gcccgtcacc acaccgaaca gaccgcggcc cccgttcgcg
    88261 agcgggttgc ggagctgcgt ctgcgcgttc aggccgccga gctggccgag gcccagttcg
    88321 ccacccgtct ggaggaagcg caggccgacg aagccgccct cgcgccgctg ctcacggcgg
    88381 acctgaagga aggcgccttg cagcgcgaag tggcccgcct tggccgcgag atcgcggaac
    88441 tgggggccgt gaacctggcc gcgctggacg agctcagggc ggcgtccgag cgcaagacct
    88501 acctggatgt gcagagcgag gatctcctgg gggccatcga gaccctggaa gacgcgatcc
    88561 gccgcatcga ccgggaaacg cgggagcagc ttcaggagac gtacaatacg gtcaaccgcc
    88621 atttcggcag cctgttcccc cagcttttcg ggggcggcga ggcgcggctg gtgctgactg
    88681 gcgacgagat tctcgacgcg ggcatccaga tcgtcgccca gccgccgggc aagaagaaca
    88741 gttccatcca gctgctgtcc ggtggcgaga aggcgctgac ggcgattgcg ctggtgtttg
    88801 ccatgttcca gctcaatccg gcgccgttct gcatgctgga cgaagtggat gcccccctcg
    88861 acgatgccaa taccgggcgc tattgcggga tggtgaagcg gatgtcgtcc cagacccagt
    88921 tcatcttcat cagccacagc aagatcacga tggagatcgc ccagcagttg gtgggcgtga
    88981 cgatgcagga gcagggcgtg tcgcgggtgg tggaagtgga tatcgaagaa gccatgcgcc
    89041 tggccgaaac ggcggcgtga gtgtgaaaca aagggaagca gatgggaatg agtgaattgc
    89101 agatgggcct gatcggggcc ggcggtgcgg tcgtgctggg catcttcgcg ttcaacaagt
    89161 ggcaggagcg ccggcagcgc aaactcgccg agccgcctgt gccgacggag ccccaggctt
    89221 ccgggacggc ggcccgcggt ggcgaggcgc gtatcgagcc ggtgctcggt gcggcgacgc
    89281 cccggcccgt ggccgcccgt ccggcgccca ccgccggacc gcgtcgcgtg ccgccgccct
    89341 tgccggccga ggtggacgag cgggccgact gcgtggtgcg agtggaatcg atcgagcccg
    89401 tgcagggcag ccgcctgctc caggccgcag cgcagcatct gcagggcatc gccaagccgg
    89461 tgcgctggtt cggcttttcg gatcacgaca atcgctggga aacgctggat ggtgcgcccg
    89521 gccacggcta tcactggatc tgtgcggcgc tgcagctggt ggatcgccgc gggccggtga
    89581 gcgaagggga tctgtcggcc ttctgcggcg gactgcagcg catgtgtgac atcttcatgg
    89641 gcattccgtc cctgccggtc aaggccgaag ccctggaccg ggccgcagag ctggatcgcc
    89701 tgtgtgccgg cgtggacatc cagatcggcg tcaatgtggt ggcccgcgaa ctgccgattc
    89761 ccggcaccaa gctgcgcggc ctggctgagg cggccgggct ggaactgggc acggacggct
    89821 gctttcacgc ccgtgacgat ctgggcctga gccagttcgt gctgagcaac ctggagccgg
    89881 tccgattcgt ggccgaggag atgcgtggcc tgagtaccca gggcgtcacc ctgacgctcg
    89941 acgtgccgcg ggtggcggat ggcgtgaagg tcttcgaccg catgatgacg gtggcccagc
    90001 aactggcgga agccctcaac ggcgccgtgg tggacgacaa ccgcacgcct ttcggtgaca
    90061 aggctgcggg cctgatccgc gcccagatcg agcagtttca ggtgcagatg ggcgagcagg
    90121 gcatcgcgcc gggttcgaca ctggcgcggc gcctgttcgc ctgaccgcgc gccgtgtccg
    90181 tcccggccga acagatcgcg cgggcggcgg ccctgcgcga ggcgctcgaa cgctacaacc
    90241 acgcctacta tgtcctcgat gagccccttg tcccggatgc ggaattcgat gcccgcttcc
    90301 gtgaactgca ggcgcttgag gcgacgtatc cggaactcct gacacccgat tcgccgaccc
    90361 agcgtgtcgg cggccagccg gcgccggagc tggcgccggt gagtcacgcg gtgccgatgc
    90421 tgtcgatccg caccgagacc gataccaccg ccgaaggcgc cgttgtcttc gacacacgcg
    90481 tgcggcgggc cctcgggctg acggacgacg cgccaccggt gacctacgcg gcagagctga
    90541 agttcgacgg gctcgccatc aatctgcgct acgaaggtgg caccctggtg ctggcggcca
    90601 cccggggcga cggcacgacg ggcgaggatg tgacgcgcaa tgtgcgcacc atccgccaga
    90661 ttcccctgcg gctgaagggc gcgctgccac cggtgctgga agtgcgcggc gagatctaca
    90721 tgcgccgcga cgcgtttgac cgtctcaacg cggaccaggc agccgccggc cagaagacct
    90781 tcgtcaatcc ccgcaatgcc gctgccggta gcctgcgcca gctcgacccg gccattacag
    90841 cgcggcggcc cctgtccttc tttgcctacg ggctggggga ggtggtgggc tgggaactgc
    90901 cgccgaccca cgccgagctg ctcgatgcac tggacgccct gggcttcccc gtctgcgtgc
    90961 accggacgcg tgcccaaggg gcggcagcgc tcgcgcagtt ccacgaggcg atgggcgtct
    91021 tgcgtgacgg cctgcctttc gacatcgacg gcgtggtcta caaggtggac agtctggccc
    91081 tgcagcaggc gctgggtttc gtctcgcggg agccgcggtg ggcggtggcg cacaaatatc
    91141 cggcccagga ggccgtgacg tatctgctgg gtatcgacgt tcaggtcggc cgcaccggcg
    91201 cgctgacccc ggttgcccgc ctggagccgg tgtttgtcgg tggtgtgacg gtcaccaatg
    91261 ccacgctgca caaccagggc gagatcgacc gcaaggagct tcgcgtcggt gaccgcgtgg
    91321 tggtgcgccg cgccggcgac gtgattccgg aagtcgtcgg ccgtgtgatc ggggacgagg
    91381 aggaggaggc ggcccacgtg gcccgtccgc gtttcgatct gcttggcact tatccggtgt
    91441 gcccggagtg tggctcccac gtggtgcggg gcgaggacga ggcggtggcg cggtgtaccg
    91501 gcggtctgta ctgcccggcc cagcgcaagc aggcgctgct gcactttgcc ggacggcgtg
    91561 ccatcgacat tgatgggctc ggtgagcaga tcgtccacca actcgtgacg aattccacgg
    91621 tcaccacgcc ggcagacctg tataaccctg accggatcaa tgccaatgta cttgccgggc
    91681 tggagcgcat gggcgaaaag tccgccgcga acctgatggc ggccatcgag gcgagccggg
    91741 agacgagtct ggcgcgcttc atcttcgccc tggggatccg caatgtgggc gagaagaccg
    91801 cgaaggatct ggcccgtcat ttcggtggac tgaaggcgct gatggctgcc gatgaggcac
    91861 agctgctgca ggtgccggat gtcgggccgg tggtggcgcg gtgcatcgtc gagttcatgg
    91921 gcgaagcccg taatcaggcg gtcatcggca gtctggccga cgccttgcac tggaccgagg
    91981 gcgctccggc ggcccgctcg gccgacggcg gcctggcggg caagaccttc gtgctcacgg
    92041 gcaccctgcc cacactcaag cgcgacgcgg cggcggcatt gatcgaagcg gcgggcggca
    92101 aggtcagcgg ttcggtttcc aagaagaccg actaccttgt cgccggagcc gaggcgggca
    92161 gcaagctcga caaggcgcaa caactgggtg tcatgattct ggacgaggcg gggctgatgg
    92221 ccctgcttga gccggtgccg cccgcgcagg acgggggcgg tcagggaagc ttatttgagg
    92281 atcaggaatg aagaaggtta cgaaagcggt atttccagtg gcgggcatgg gcagccgctt
    92341 cctcccggcc accaaggcgt cgcccaagga gatgatgccg gtggtggaca agccgctgat
    92401 ccagtacgcg gtggaagagg cggtggcggc cggcatgacg gatctggtgt tcatcaccgg
    92461 gcgctccaag cgggccatcg aagatcactt cgacaaggct tacgagctgg agaccgaact
    92521 cgaggcgcgg ggcaagacgg aattgctcaa gatcgtccgc gagacggtgc cggcgaatgt
    92581 gaactgcatc tacatccgcc agccggaagc gctgggtctg gggcacgcgg tgctgtgcgc
    92641 ggcgccggtg gtgggcgatg agcccttcgc cgtgctgctg gcggacgacc tcctggacgg
    92701 tcagggcgcc tcatcggtga tgcgtcagat ggcggatgtg tatcactacc atcgctgttc
    92761 ggtgctgggc gtcatgaacg tgccgcggga gcacacccgc cagtacggca tcgtcagcac
    92821 cgagcgccag gacggcgatg tgcagcgggt gacggggatc gtcgaaaagc ccaagcccga
    92881 agaggcgccg tccacccagg cggtggtggg gcgatacgtg ctcaccggcc gcatcttcga
    92941 acatctgcgc aagctcacgc cgggcgcggg gggcgagttg cagctcacgg acgccatcgc
    93001 ctccctgttg cgtgaagagg cggtgctgtc ctaccagttc aagggtgtgc gttacgactg
    93061 cggctccaag ctcggatatc tgcaggccac cgtcgagctg ggcctgaagc atccggaagt
    93121 gggtgcggag ttcgcggcgt atctggcttc acgtgccggt gtggacggcg cctgaccggc
    93181 gcacacgccg gcagatgaca tgagaggccc gcccgcgcgc gggcctcttc gtttccgact
    93241 ggcgctctgc aaggcgcacc cggcgtcagc gttccgtgag ggcgttggcc ttggcccgca
    93301 ggacgggctt gaggaggtag ctcagcagtg acttcttgcc ggtgagaatg tccacctcgg
    93361 ccaccatgcc tggaatgatg ggcagcctgg catcgcccag ggcactatgg acggtgcgta
    93421 cgcgcaccac gtagtaggca ttgccctttt cgtcggtgat ggtgtcggcg ccgatctgtt
    93481 ccagtgtggc gtcgaggccg ccgtagatgg cgaagtcata ggcggtgaac ttcaccagcg
    93541 ccggctggcc gggacgcagg aaggcgatgt ccttgggctg aacgcgggct tccagcagca
    93601 gggtgtcgtc gctgggcacg atctcgataa tctccttgcc cggctggacc accccgccca
    93661 cggtgttggc gaagagctgc ttgacggtgc cgttcatcgg cgcgcggatc tcggcctgct
    93721 tgacccggtc ggcgaggcct tcgctgccgg cggaaagggc ggcgatcttg gcgttggtgt
    93781 cggagagctc gctgcgcgcc tgattgcgga tctggagctc cacttcctcc accttgttct
    93841 gggcttcggc gatggcggcc tggatgcgcg ggatctgggc gacggcggcg ttgcgttcgc
    93901 ccttgaagcg ggccacgtcc cgttccaggc gcaggagctc cacctcggag acggcgccgg
    93961 tcttggccag cgggcgggtc acttcgagct cgcgggcggt gaggtcgtag ctctgggacg
    94021 attgatcccg gcgggcgctt acttcagcca gttcctggcg gcgctgggac agttgctggc
    94081 gggcgatgct ggcagcggcg tccatctcgg cgcgcttgga ttcgtaggca ctgcgctcct
    94141 gctccaccag atccggcgcg tccttgagca cctcgggcgg ggcgacgaag ggcttgccgt
    94201 gggagatggc ggtgagacgg gctgccttgg ccagcagtgc cagatattgg gcgcggtttt
    94261 cccgcaggga cgagacgaag cgggtcgggt ccaccttgag gagcagctcg ccggccttca
    94321 cctgctggcc ttccttgacg aggatctcgg agacgatgcc gccgtccagg ctctgcagga
    94381 tctgcacctg cttggacggg atgaccttgc cttcgccgcg ggtgacctcg tcgagctggg
    94441 caaacgcggc ccacagcaac aacacgagca cggcgaccag ggtgatccac acggcgatgc
    94501 ggctgccgcg caggcgctgc tcggccatcg cccattcggc gtcggcgagg tagtcggtgt
    94561 cgtcgtggat ctcgccccgt tcggcgccat ccagcaaacg gtcgaaggcg cgggagctcc
    94621 gatcatagac ccgcgcgtac tgaggctgaa tgaaggcggc cagacgggca aacagggact
    94681 tcatcgcatt agcctccccg tccgatgcgg ccctgttgca gggcttcgac cacctgggcc
    94741 ttgggcccgt cggccacgat gtggccctgg tcgaggacga tcagccgatc cacaaggtcc
    94801 agcagcgagg tccggtgggt gacgaggacg atcgtcttgc cggccgcgaa agcgcgcagg
    94861 cgctgcttga gttgctcttc gctctggtgg tccatgctgc tggagggctc atccagcagc
    94921 aggatgggcg gatcgttgag caccgcacgg gcaatggcca cggcctggcg ctgcccgccg
    94981 gagagggatt cgccgcgttc gccgatgggc atgtcgaaac cctggggatg ggcgtcggcg
    95041 aactccttga cgccggccag ctcggccgcg gcgagcactg ccgcgtcatc ggcgaagggg
    95101 gcgcccatgg cgatgttctg tttgaggctg ccgtagaaga gggtcacgtc ctgggggacg
    95161 aagccgatgg cgcggcgcag ttcggccggg tccacctggc gggcgtcgat gccgtcgatg
    95221 aggacggcgc ccgcggtcgg ctgatagagg ccgaggatga gcttttcgat ggtggtcttg
    95281 ccggagccga tgcggccgat gatggcgacc ttctcgccgg cctgcaggcg gaaggagatc
    95341 gtgtgcaggg ccgcctgatc gcggccggga taggcgaagc tcacatcctt gaactcgatc
    95401 tcgccgcgga aggcaggccg atggacgaag ttggcgccgt ccgggcgctc gaccggcagt
    95461 tgcatgtggc cttcgacggt ctccagcgcc gtgcgggcgt tgtggtattg catcagcagc
    95521 cccgccacct ggccgagggg ggccatggtg cggccggcca gcatggaggc cgcgatgatg
    95581 ccgccgaggc tcatcttggc gtcagccagt tcgtacacgc cgacgatgat gacggcgacg
    95641 gagacgaact gctgcatgaa ctgggcgaaa ttcaccgtac cggcggagag cagcttgagg
    95701 cgtccgccga tctgggcgat gaagagggtg gcgcgttcgt actggcgctg gatgaggcct
    95761 tcggcggcga gggtcttgag ggtttccagg cccaccaggc tttccaccag ggtggcgttg
    95821 cgctgggcac cggcgcggta ggtggtctcg gtaagggcat gcattttgtc ctgggcgatc
    95881 agggtcacga acagcaccac gaggatgccg accaccggcg ggatcatcag ccacggggag
    95941 atccacgcca tggccaggag gaagagcagc acgaagggca ggtcgatcag ggtggtgacg
    96001 gtggccgagg cgatgaagtc ccgcacgccc tcgaaggccc gcagattggc agcgaaggag
    96061 ccgaccgagg ccggccgcgc ctccatgcgc agacccagca cgcgctccat gatgcgggcc
    96121 gacagctgca cgtcgatgcg ctttccggcg gtatcgacga tgtaggcgcg cagcgtgcgc
    96181 agggcgaagt cgaagcacag cagcaaggcc gtgccgactg ccagcaccca cagggtttcc
    96241 acggcccggt tggggacgac ccggtcgtac accgtcatgg aaaacagggg gatggccagc
    96301 gcgaagaggt tgatgacgag ggcggcgagc agggtgtccc ggtacagccg ccagttgtcg
    96361 aggacggtgc cccagaacca gtgctggccg cgcagactgc ggatctccgg cgcacgggcc
    96421 tcgaagcgga agcgcggacg gacaaagcac accagccccg tgtagcgggc ggcgagctcg
    96481 tcggcgctga ggacgtccac cgattcgccg gcttcgggaa atcgcacccg gcaggcgccg
    96541 tccggcaggc gttcgaggag caggcaggcg cggcggtcgt gaaggagcaa cagcgccggc
    96601 aggaaattcg gggtgatctc cgccagcggt ttgcgcacga tgcgcgcggt gagctgggcg
    96661 cgggcggcgg cgcggggcag cagggagggc gtcaggcggt gatcgacgag gggcaggccg
    96721 gccgagaggg cttccggggt gaaggggttg ccgtggatgc gggtgaggag gaccagcgcg
    96781 tcgagcaggg ggtcgtcgtg ggtggcatgc tcgccgatct gccagccgga acggctcgtg
    96841 ggattcgtca tggtcgggct gcgggatacc ggtatggccg aaggagggcg cctgcgcgcc
    96901 gctccgcatc ctaacggaag cgagaaggtg aacttaagcc ttcaggcaaa aaatctcggt
    96961 gtcaacgacg cggcggcccc tgggcgcgac cactgcgtcg cgctcagatg acgtcgtgct
    97021 tgccgaggag gttgagcccg ttgcgcaggc gttcccgcgc acccttgcgg gcgaggagct
    97081 tgcgctgggc gtcggggggc aggtcggtga ggcgcagcag gccccggtcg atgaggacgt
    97141 ccaccacgtc ctcgatgacg cggatcaggt cggcgtcgag ggcggtgaag gtgaccgcgc
    97201 cgtccgctgc gccacgcccc ccgaggaagg cggccacctc cgggtggtcg gccggcagct
    97261 cttcggagtg ccccggcccg gcgtcggcaa acagggcgac gatgcagcct tcggcgttgc
    97321 gctggacgaa gctcatggga cggtgggtgg agtggcggtg ccgtattgtg gccgtggcgc
    97381 ccgtcttccg tcgagggggc gagcagcttg acggcggcag atcaggcggc cggcgtctgg
    97441 agttccctgg gcagcggaaa ggtgaccttc tcgggcacgc cctcgagctg tcgcacggag
    97501 gccgccccca aagccttgag gcgggcgatc acggactcca ccagcacctc cggcgccgag
    97561 gcgcccgcgg tgacgccgat gcgctgcttg cccgtgagcc aggccgggtc gatggcgtcg
    97621 gcgttgtcca ccaggtgggc aggtacctga agcagctcgg ccacctcccg caggcggttg
    97681 gaattggagc tgttgggcga gcccacgacc agcaccagat cggactgggg cgccatgaat
    97741 ttgacggcgt cctggcggtt ctgggtggcg tagcagatgt cgtccttctt ggggccgacg
    97801 atggccgggt agcgttcgcg cagggcgccc acgatggcgg ccgcgtcatc caccgacagg
    97861 gtggtctggg tgacgtaggc aagggcgtcc ggggtgttca cctgcagggt cgccacgtcg
    97921 ccgacggatt ccacgaggtg gatgccgtcc ttgacctggc ccatggtgcc ttccacttcc
    97981 gggtggccct tgtgaccgat catgacgatc tcgcggccct ggtcgcgcat gcgggcaacc
    98041 tccaggtgca ccttggtgac cagcggacag gtggcgtcga agacgcgcag gccgcgggcc
    98101 tcggcttcgc tgcggacaga ctggggcacg ccgtgggcgc tgaagatcac cgtgttgccg
    98161 gccggcacgt cggccagatc ctcgatgaag atggcgccct tggcacgcag gccttccacc
    98221 acgaagcggt tatggacgac ctcgtggcgc acgtagatgg gcgcgccgaa gcgggccagg
    98281 gcccgttcga cgatctcgat ggcccgttcg acgccggcgc agaagccgcg gggagtggcg
    98341 agcaggactt ccatgggaac ctcagttgat gccgatgatc tggacttcga agcggatggg
    98401 cttgcccgca agcgggtggt tgaagtcgat caccgcgtgg gtgtcgagca cctcgcggat
    98461 caggccggga tagcggctgc cgtccggcgc ggtgaattcc atgatgacca tgggttcgac
    98521 ttcggcgcca tcggggaagt cttccaggcg caccttctcg accagttcgg cgcggtgggc
    98581 gccgaaggcg ttctcgggtg ccagttcgaa gctgtggcgt tcgcccacgg cgaggccggc
    98641 gaggcagcgt tccagtgtcg gggcgatgtc accctggccc agatgcatcg tcgccggctg
    98701 ggcctcgaag gtgctgatga ggggctggcc gttgtccagg ctgatgcggt agtggagggt
    98761 gacgaggctg tcgggctgga cggtctgggt catgaaaggg tctctcgggt gggcgcgtga
    98821 cggtgttcgc gcagttgggc gatgaccatc agcgttacgc cgacgcagat ggcggaatcg
    98881 gccacgttga aggccggcca gccgtagcgg ccgacgtgga agtacaggaa gtcgaccacc
    98941 gcgccgaaca cgaagcggtc gatgacattg ccaatggcgc ccccgatgat cagcgagaag
    99001 gccagcggtt gcaggaattc atgctggtgc cgcgccacca gacgcagcag ccagccgcag
    99061 atgaccgccg ccagggccac gaagaaccag cgctgccagc ccgcctggtc ggcgagaaag
    99121 ctgaaggctg cgcccggatt gaaagccagg accaggtcga agaaatcggt gacggggatc
    99181 acctcgccgg tgcgcagggc gccgcgcacc aggtgcttgg tccattggtc cagcaggacc
    99241 acgacggcgg cgagtccgat ccagttgccg aggcggagtt tcacaggcgg caggctccag
    99301 ggtgatcagg catgtgcgcg ggtttcgcca gtgccgtgca gattggacgt gcagcggccg
    99361 caaagttccg gatgagcggg atcatgaccc acatcgtcgc ggtagtgcca gcagcgttcg
    99421 cacttgcgct gggccgaggg actgaccacg atggccgcgt cggcctcgct gtctacgcgg
    99481 gtgaggacgg tcttcgagca gatcagcacg aagcgcaggt cgtcgcccag gctggcgagc
    99541 aggtcgtact gggcgcccgc ggcgcgcagc tccacctcgg cctgcaggga cgagcctagc
    99601 ttgccgtcgc tgcgcacggc ctcgatctgc ttcatgacct cggcgcgtac ggcgcggatc
    99661 gcctcccagc gggccagcag ggcgtcctct ccgtcctgcg tgggcagggt gtggaaggtg
    99721 tggagcatca cgctgtcgtc ggcatctttg cccagcagca gccagatctc ctcggccgtg
    99781 aaggagagga tcggggccat gagtttgacc accgcctgca ggatgtgcca cagggcgctc
    99841 tgggccgagc gtcgcgccgc ggacgtggcg gcggtggtat ataggcggtc cttcaggatg
    99901 tccaggtaca cggcgcccag gtcttcggcg gcgaagttct gcagggcctg cacgacgcgg
    99961 tggaattcaa agcgggcata gtcggcctcc gcctgctgct gcagccggcg ggtgagggcc
   100021 agggcgtagc ggtcgatctc gagccattgg tccaccggca gcatgtcggt ggccgggttg
   100081 aagtcggcgg tgttggcgag gaggaagcgc agggtgttgc gcaggcggcg atagacctcg
   100141 acgacccggt cgaggatctc cttggagatg gacagctcgc cggagtagtc ggtggacgcc
   100201 acccacaggc gcaggatctc ggcgcccagt ttgccggtga cttcctgggg cacgaccacg
   100261 ttgccaaggg atttgctcat cttgcggccg ctgccgtcca ccgcgaaacc gtgggtcagc
   100321 agggcgtgat agggcggatg gccgtcgatg gcgcagccgg tgagcagcga tgagtgaaac
   100381 cagccccggt gctggtcgga gccttccagg tagaggtcgg cgcgggggcc ggtggcgtgg
   100441 ccgtccggat gggagccgcg cagcacgtgc cagtgggtgg tgccggaatc gaaccacacg
   100501 tccagggtgt cgctgatctt gtcgtactgg gcggcctcgt cgccgaggag ttcggcgggg
   100561 tcgagcttga accaggcttc gatgccttcc tgctcgaccc gcgtggcgac agcttccatc
   100621 agttcgacgg tgcgcggatg gagttcgccg gtggccttgt ggaggaagaa ggggatgggc
   100681 acgccccagt tgcgctggcg cgagatgcac cagtcggggc ggttggcgat catggcgtgc
   100741 aggcgggcct ggccccaggc ggggaagaag cgggtcgcct cgacgccctg cagggccagc
   100801 tcgcgcaggc tcgggccgcc ttcgggcttg aggtccatgc ccacgaacca ctgggcagtg
   100861 gcgcgataga tcagcggcgt cttgtggcgc cagcagtgca tgtagctgtg gc
//
LOCUS       DAOQMM010000002        91915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_28_length_91915_cov_51.227814, whole genome shotgun sequence.
ACCESSION   DAOQMM010000002 DAOQMM010000000
VERSION     DAOQMM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 91915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 91915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91915
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28_length_91915_cov_51.227814"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..1675)
                     /gene="rmuC"
                     /locus_tag="PLH85_00485"
     CDS             complement(329..1675)
                     /gene="rmuC"
                     /locus_tag="PLH85_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNB63063.1"
                     /translation="MLPWLLAALGLSLVLLVWLAFQVAALARRQDAVAQDLADSLADR
                     VEHLLGHQQLQFVKELQAATSAATDRVTDLTAEQGERLQARLATQREETALRLAELGA
                     GVAQGQERLRSELLAETLRTLSEHARADRELLQSGLQAASAQLTHGIEGLGRTVGERL
                     DAIGGHVNQRLDEGFRKTNETFVNVMARLATIDEAQKKIGDLTTNVVSLQELLGDKRA
                     RGAFGEVQLEALVRNALPPDAFDFQYTLPNSTRADCVLRLPAPTGLVVVDSKFPLENY
                     HRMFAADQGDADRRTAQAAFRADVKKHVDAIASKYILPGVTSDGAVMFVPAEAVFAEI
                     HAYHPEVVTHALAKRVWIVSPTTLMAVLNTARAVLKDVETRRQIHVIQDALGKLAKDF
                     ARFDERMQKLATHIRQAGEDVADVQTSSRKITAHFQKIEAARLDDLPPALPELDGD"
     assembly_gap    1759..1858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1933..4428)
                     /locus_tag="PLH85_00490"
     CDS             complement(1933..4428)
                     /locus_tag="PLH85_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB63064.1"
                     /translation="MTVRQRLALSFRMLRRDLRAGELGLLLVALVIAVASLTSVGFFT
                     DRVGRGLNRQASQLLGGDLLIAADHPLAPDFRAEAARRGLASVESTTFTSMASLGDAT
                     QLVGVKSVEAGHPLRGEIRIAPGLNQPDAPARGIPAPGEVWLDERSATALGAHPGDLV
                     GLGRSRLRMAAVLSFEADRGANFFALLPRIVMNAADLPATGLIVPGSRVTYRLHLAGS
                     IAAVAGYEAWAKARLGRGESLESVDNARPEIRATLDRTERFLRFAAMLAVVLAAVAVG
                     LATRRFVERHLDGCAVMRCLGARQTTLLGLFIGEFTLLGLAGGLAGSALGFGVQYALE
                     RFLGSLVSFELPPPSVWPLAHGLAVSLVLLLGFALPPLIRLGRVPTLRVLRRELGGVA
                     VRDGLAWSLGAAVLTGLLMLVARDVTLGAWVTGGFVAAFGVFAGVGWIVLRGVGRIRA
                     GAGSGWRYGLAALRRRPGASLVQVLALGMGLTALLLLTLVRGDLLASWQQATPPDAPN
                     RFIIGIQPDQLDPLRDAFRAAGRPAPVLLPMIRGRLVAVNGRPVGPDDYTDDRARRLV
                     DRDFNLSHDAALPPGNRVIAGTWHGHDTTPRFSVEQGLAQTLGLRMGDVLRFNVAGEI
                     VEAPIASLRKLDWDSMRVNFFVIASPGLLADFPASYITSFYLPPAQTDFVPRLVARFP
                     NFTVIDVAAMLAQVQGMVDQLVAAVQFVFGFAVVAGVVVLLGALQSTHDERERELAIL
                     RTLGARNAQLRAALLAEFAVLGVLSGSLAGVAASGIGWSLGRFVFTLPYTPSLLPVLV
                     GALAGGLAVTLAGWFGTRRLLLTPPLASLRALG"
     gene            complement(4662..5525)
                     /locus_tag="PLH85_00495"
     CDS             complement(4662..5525)
                     /locus_tag="PLH85_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB63065.1"
                     /translation="MKRIPSQTALTFALLIGSVAGAHAATAWTLTSGGTETGAGNFGN
                     TRTYGSTGSTVTISAWSDTKNSTTAGNAYIESAYLGTYSGGLGVTNRDGATGAGDTNE
                     GTIANTTSPGHTMDNNARYDSMLFDFGAGNAVKLQSVTVGWWYSDSDLTVLAYTGTGT
                     PTFTSANQGYASLLSSGWTTVKSSAGTTGTANYADAAGGAPSQSSNSAGYIPLAVNDL
                     NVSARYWLIGALNTLVQALPNGATLGSGNDYIKIAAVTGTYTKVPEPSSLALLGGTSL
                     IALLGMRRRKN"
     gene            complement(5688..8507)
                     /gene="prsT"
                     /locus_tag="PLH85_00500"
     CDS             complement(5688..8507)
                     /gene="prsT"
                     /locus_tag="PLH85_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNB63066.1"
                     /translation="MPTSQQPHVTLRRLPAALLCLCLTLPGWAAGASDSATRYYEDAA
                     ARLARNDAAGAIIQAKNALQRDPEQLPAHLLLARALLKDGQAAAAEAEFEAALRLGVN
                     PSEIALEYGQALMMFGNSEKLLERIKADGLPLRLRAEVLAMRATAQADRGDMRAAVAE
                     VKLAREADPRALSPLLAEIDLALRSRDSARARQVLDTALGIAPGDARLLHLQAGLLQG
                     AGDARGAMEAFNRAIAADPRMLDALVGRASLLIDLKRANEALPDLERAAALVPREPRV
                     AYLKSLVYAQRGDTQASRAQLEDVVRLVDELPQEFVGRQPTLLMIGGLASQALGRGER
                     ARALLELYVRRMPGDAAGRKMLANIYLAAGETARVGDLLQPLISSGDADPQALTTLAA
                     LRMQQRRYRDAADALEAAARLTGNPGITAQMGFARIANMQPDIGLSALRSAFDKEPGQ
                     LNVATALSTLYLRRGDLRNALAVAEATTRRLPSDPAAHNLLGAVKGAARKPAEARAEY
                     TQAITLAPGFLPAQLNLARLDLVEGRADAARQRLAALLKAHPADVQVLTENARLELGA
                     GRSADALALLEKAYALKPADPTVAQELLALYQKLGKPAPAVAVAKQLALSRPDDPLVL
                     ETLGRAHLAANNLADARSTFNTLSRLPGLEPGQLVAVGRLQLDAGAFREASASAEKAL
                     ARNAGLVPAQILQIESEMALGDLNRADALLKSFATRSGNSAEALRLAGDLARARQQPR
                     DAARLYRSAFDKAPSLALVLRGVEASFQAGEGDRAVSLLDTWLRTHPNDLTARSALAE
                     SHFRLGHLADARRLYEGVLAAEPANANAANNLAQILMALNDPGALNAAEAAYRLAPGD
                     ANVLDTVGWARARSGALDIGLKALREARLRAPESREIRYHLAWVLLRKGLRDEARAEL
                     QGALNSTNGSGYDIEIQNLRRELGV"
     gene            complement(8771..9940)
                     /locus_tag="PLH85_00505"
     CDS             complement(8771..9940)
                     /locus_tag="PLH85_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HNB63067.1"
                     /translation="MHLLDSLRADHADITALRRDIHAHPELAFEEHRTAGLVAERLRA
                     LGIETHTGIGKTGVVGVLRAGSGRRAIGLRADMDALPIQEKNDFAHHSRHPGRMHACG
                     HDGHTAMLLGAAAHLSRHPEFDGTVYFLFQPAEEGEGGAPEMIADGLFERFPMDAVFG
                     LHNWPGLPLGEIAVHRGAVMACADRFDIEIRGHGAHAAMPHQGIDPVLAGAALVQALQ
                     SVVSRGTDPQDAVVLSVTQFQAGDAYNVIPDVARIGGTVRAFRPELQARVEDAMQRIC
                     AGVGAAFGANVHFEYRRGYPPTINSVAEAEFCARVAAEVCGDARVSVDPRPSMGAEDF
                     SYFLQEKPGCYIWLGNGPGEGGCTLHNPHYDFNDAAIPYGVAYWVRLVQRWLADA"
     gene            10011..10931
                     /locus_tag="PLH85_00510"
     CDS             10011..10931
                     /locus_tag="PLH85_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNB63068.1"
                     /translation="MKLFYADHFVLPLPPGHRFPMEKYARLRERLQASGLFRPDDFHE
                     PPPATDTEILRAHDAGYLQRVVRGQLTPDELRLIGFPWSPAMVERSRRSSGATLAACR
                     AALEDGCAVNLAGGTHHAFRDRGEGFCVFNDAAIAALAMRAEHRVERLAIVDCDVHQG
                     NGTAAILAHRADCFTCSLHGAKNYPFDKERSDLDVELPDGTADGPYLDALAMALETVF
                     ARADPQLVIYLAGADPYADDRLGRLALSLDGLAARDRLVLGECRRRGVSVAIAMAGGY
                     ARQIDDTVAIHARTVTLARQRFAPGPTEAP"
     gene            10928..11770
                     /locus_tag="PLH85_00515"
     CDS             10928..11770
                     /locus_tag="PLH85_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB63069.1"
                     /translation="MSQESHIALLIDADNCPAAKIEEILDELAKYGVINIRRAYGNWK
                     SPGLKSWEAVLHEYAIQPMQQFAYTKGKNATDSAMIIDAMDLLYSQNLDGFCLASSDS
                     DFTPLVMRIRANGLKVFGFGEKKTPSPFVNACSKFLYLENLGATDADTPASEVPPAAV
                     PSARAEPDGGAGRGAAAGDAGKAAESSTRPVARPTPRELRGDARLVNLLRKSVEAAAD
                     DEGWAALGEVGQHISKQASFDSRNVGYARLRDLMQDIGLFDVSQRDKAIYVRDKRLAR
                     KPAP"
     gene            11838..12680
                     /locus_tag="PLH85_00520"
     CDS             11838..12680
                     /locus_tag="PLH85_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB63070.1"
                     /translation="MRFEGTETYVATPDLMLAVNAAVTLQRPLLIKGEPGTGKTLLAE
                     EVAAALGMPLVQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDRVKDIGQYIVRGPLWQ
                     AFESDVPCVVLIDEIDKADIEFPNDLLRELDRMEFYVYETRQLVKARHRPIVFITSNN
                     EKELPDAFLRRCFFHYIKFPDRDTMQKIVDVHFPGLKPALIQEALQVFFGLREVPGLK
                     KKPSTSELIDWLKLLVAEDIPPEALHSTDRKVIVPPLHGALLKNEQDMHLFERLVFMA
                     QRNR"
     gene            12691..13869
                     /locus_tag="PLH85_00525"
     CDS             12691..13869
                     /locus_tag="PLH85_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB63071.1"
                     /translation="MLIDFFLHLRERKLPVSTKEFLTLLEALQAGLARHSLDDFYFLA
                     RSCLVKDEAHFDRFDQAFGEYFKGVQAIPGLDVDLPDEWLKATMKRHLSPADKAKLEK
                     LGWDTLMAEFKKRLQEQRERHAGGSKWVGTGGTSPFGNSGYHPEGIRVGGESAGNRTA
                     VKVWDRREYRNLDDSVELGTRNIKVALRRLRRFARLGAPDELDLDGTIAATARNAGYL
                     DLLMRPERHNAVKVLIFFDVGGSMDPHVKVCEELFSACRAEFKHLEYFYFHNCVYDHV
                     WRDSTRRYTERTPLADVIHKYGPDYKLIFVGDATMSPYEILQPNGSIEYASSEAGAVC
                     LRRLADAYPALAWLNPEPERTWEFRQSIGIIRELLGERMFPLTLDGLTRAIAHLSRKH
                     "
     gene            complement(13936..14706)
                     /locus_tag="PLH85_00530"
     CDS             complement(13936..14706)
                     /locus_tag="PLH85_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB63072.1"
                     /translation="MAVELFNDGKHACLAFYDLVDEEADHAVQCNQFLIVDGEHGALV
                     DPGGNMTYNGLLMAMQRFFPSRGLDYIFATHADPDIIASLNKWMVTTHCRVMISRLWA
                     RFVPHFTTGKDYTSRITGIPDEGMNIALGDSRIKALPAHFMHSEGNFQFYDPVAKILF
                     SGDMGTSLVDHAQAAEPVRNFDDHVITMAGFHRRYMISNKICRYWVHMVRQLDVDMIV
                     PQHGCRFEGKAMVNRFLDWIENLPCGIDLMTQDNYRAP"
     gene            14992..15531
                     /locus_tag="PLH85_00535"
     CDS             14992..15531
                     /locus_tag="PLH85_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63073.1"
                     /translation="MGMSSGFGSSGMGSANTLSGGIGTRSLLDSKQNGPQIKDLRRPS
                     ASECAMRELAGKKSPGMTANGQAGSGKVTTYTDASSLRGRSGDIYGDCDGAQGMRRRG
                     AGGSTGTSSFVGIGSATRPQTAYDRRTGTLKAAKDGYVNRGATSSMMPKHPESAYDPV
                     TGTLKPPRVQSAYQIYQQQ"
     gene            complement(15632..17551)
                     /gene="htpG"
                     /locus_tag="PLH85_00540"
     CDS             complement(15632..17551)
                     /gene="htpG"
                     /locus_tag="PLH85_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNB63074.1"
                     /translation="MAVEQMSAQTMNFQAEVKQLLHLMIHSLYSNREIFLRELVSNAS
                     DACDKLRFEALENGALLAEDAELKIRVAYDKAARTLTIADNGIGMSRDEVVTHLGTIA
                     KSGTKEFFGKLTGDQQKDAHLIGQFGVGFYSAFIVADTVTVRTRRAGLSAGDAVKWEC
                     TMTGDTAGQYTVEPIEKASRGTEITLHLREDQDDLLSAWKLQSIIRKYSDHILQPIVM
                     KKESWDEDKKEQVVTDEDETVNKANALWARPKSDVSDEEYTEFYKHVGHDYEPPLAWT
                     HSRVEGRHEYTQLLYVPGHAPFDMWDRQARHGVKLYVRRVFIMDDAEKLMPLYLRFVR
                     GVVDSNDLPLNVSREILQESKDIDTLRAGCTKKVLGLLEDLAENQKDKYTTFWKEFGQ
                     VLKEGVGEDFSNKEKIAGLLRFASTQADTADETVSLADYIGRMKEGQDKIYYVTADSF
                     NAAKNSPHLEIFRKKGIEVLLLSNRVDEWVVGHLTEFDGKALASVAKGGLDLGTLEDE
                     AEKAAVEKETGEFKELLDKMKGSLGDKVKDVRITHRLTDSPACLVADEHDMGMNLARL
                     MKAAGQSMPMSRPILEINPAHPVVLRLKYETGQFDDWAAVLFDQALLAEGGTLDDPAS
                     FVKRINQLMLAMNAG"
     gene            17631..19433
                     /locus_tag="PLH85_00545"
     CDS             17631..19433
                     /locus_tag="PLH85_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB63075.1"
                     /translation="MEPSHASPSGFAESDRPADVLPAGTSKGRRFSRQRIAIWGGFAL
                     LAALVAAVAVSLLRYDRAQALHRAEAELRGLTRVLEEHVARTFGETDSALSDLGQQLS
                     GRLGSDAPSERDLNALLRQRAAHLPQVEYLYVEREDGSLLADTFDLPRGAEAEAPKRG
                     LPTLHDGLEIAAPVRSAHSGHWVTPVKRQIYDAAGKFVGVVGAAMSQDYFNGVYRDLQ
                     IFPDDAILIVDATRGTLQLRHPPTDGQVGAPLGPSSALGLDANRRSAVSIEGDETPAA
                     RLTAFRRLADYPLVVGASRPLERVLAGFSDNFRRISAGAALLIALLGVLALLLHRDSL
                     RRDGDRQSLAEVNANLEERVRTRTAELEQSNRELVSFSYSISHDLRAPLRAINGYAHL
                     LDEEYGDRLDEGGHAYLARLRKASLRMGELIDELLKLANVSRQPLHIELTDVSTMARE
                     ILDEFAAQSSGRVVHIHVEDGLATEADPNLLRTALQNLLGNAWKFTRDSQPALIQVGG
                     RPHGQERLFYVTDNGVGFDLAHAGKLFEPFQQLHRNAGFEGSGIGLASVRRIVERHGG
                     SVWAESAPGAGTTIFFTLPRSPAIVRRPRERVQV"
     gene            complement(19446..19997)
                     /gene="greB"
                     /locus_tag="PLH85_00550"
     CDS             complement(19446..19997)
                     /gene="greB"
                     /locus_tag="PLH85_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNB63076.1"
                     /translation="MNKAFVKESDDDEEDAPAGAPSLPPGTRNYMTRNGFERLRAELD
                     QLVRVERPALVETVRWAASNGDRSENGDYIYGKKRLREIDRRVRFLVKRLETATVVTP
                     SEQENTEQVFFGATVTVFDASADAEQTYQIVGVDEANASEGRISWIAPLSRALLKARE
                     GDVVRFASPAGVREIEVVEIRYE"
     gene            20127..20807
                     /locus_tag="PLH85_00555"
     CDS             20127..20807
                     /locus_tag="PLH85_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB63077.1"
                     /translation="MHPILSGKRVLLTQADCFMGPALQRVFEAAGAAVIADDRPLLDP
                     ALPEALVRQAGDIDILLAHLAVPSPRTPATDVADDEWREVFAHLVDPLPRLARAVLPG
                     MVARGSGRILVLGSASALRGMRNASTYSAARGAQLAWVQALGVEVARSGVRVNAIAQN
                     FVENPTYFPPEVQANPAFQERLAREVPLGRLVSADEDARFAAYLCSDDADCFVGQVFP
                     VCGGWAVR"
     gene            20832..21890
                     /locus_tag="PLH85_00560"
     CDS             20832..21890
                     /locus_tag="PLH85_00560"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB63078.1"
                     /translation="MRLQELLSIEWPLIQAPMAGVQGSALAMAVSQAGALGSLPCAML
                     TPDALHAELAALSASTARAYNVNFFAHTPPQPDGAREAAWRTQLAPYYRTFGIDPAGI
                     ATGPGRMPFSAAIADLVEPFRPPVVSFHFGLPDAALLARVKAWGARVLSSATTVEEAR
                     WLAARGVDGIIAQGLEAGGHRGHFLDHDLTVQSGTFALLPQIVRAVQVPVIAAGGIAD
                     ADGVAAAMALGAAGVQVGTAYLLCPEATTSPIHRAVLRTESARHTALTNLFSGRPARG
                     IVNRLMRELGPLNPEAPEFPLATAAVAPLRAAAEALGSGDFSPLWCGENVSGCREIPA
                     AELTRALIQGFQRRETAC"
     gene            21911..22168
                     /locus_tag="PLH85_00565"
     CDS             21911..22168
                     /locus_tag="PLH85_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB63079.1"
                     /translation="MPCREACGACCIAPSISSPIPGMPEGKPAGVRCVQLDAADRCRI
                     FGQAERPAVCGSLRPTQSLCRGSREEALQALAWLEAHTAPG"
     gene            22322..23050
                     /locus_tag="PLH85_00570"
     CDS             22322..23050
                     /locus_tag="PLH85_00570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB63080.1"
                     /translation="MPPPQTPAATLIVPGYLGSGPAHWQSWIESRIPDTRRVRHVDWD
                     APVLARWAQAVRDEIDAAAGPLWLVAHSFGCLASVVAAADRPDKVAGLMLVAPADPER
                     FTPRGVRTSTGGVTEPGLSGCLPRRLLPMPGLVVASTNDPWVRLSSAAYWADQWGCRL
                     AEIGAAGHINADSGHGPWPLGLRLFESLRRAAGDWPLGRIDGNAQPRGGRHGALARVR
                     QLTRLASGRETGGGAPSEPSGRGG"
     gene            complement(23065..24009)
                     /locus_tag="PLH85_00575"
     CDS             complement(23065..24009)
                     /locus_tag="PLH85_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HNB63081.1"
                     /translation="MNFQQLRIIHETVRRNFNLTEVANALFTSQSGVSKHIKDLEDEL
                     GLELFVRKGKRLLGLTEPGRELSAVVDRMLMDARNIKSIAEQFSNKDHGQLTVATTHT
                     QARYALPRVVTRFKQAYPRVHLMLHQASPGEIAAMLLDGRADIGIATEALAGVSDFVS
                     FPYYGWHHAVIVPAGHALEAVTPLTLEAVAEHPLITYHEGFTGRAKIDETFAQAGFTP
                     DIVMSALDADVIKTYVELGLGIGIVASMAYDAERDRALRMLPSDHLFGENVTRIAVRR
                     GHFLRGFAYRFIELCAPELSEAVVRGALRPETGSDGPF"
     gene            complement(24009..25112)
                     /locus_tag="PLH85_00580"
     CDS             complement(24009..25112)
                     /locus_tag="PLH85_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNB63082.1"
                     /translation="MSIQVRNIHKQFGSFTALHDVSLDFPTGQLVALLGPSGCGKTTL
                     LRIIAGLEAADSGQVLLDGQDASHTHVRERQVGFVFQHYALFRHMTVFDNVAFGLRMK
                     PRGQRPSDATIRAKVKELLGLVQLDWVADRFPSQLSGGQRQRIALARALAVEPRVLLL
                     DEPFGALDAKVRKELRRWLRRLHDELHITSLFVTHDQEEALEVADRVVLMDHGKVEQV
                     GTPDEVYRHPASPFVYGFLGAVNLFHGRVDGQAVHVGDDLLSRQRVELGQGAEVLAFA
                     RPHELTIVVDPAAADGVPARVSRILAFGAVARVELDGLNGAGGQLYEVDLPRDDVHRL
                     GLKEGQAVRLQPSRLKVFERQEAAAAAAPNWVI"
     gene            complement(25114..26001)
                     /gene="cysW"
                     /locus_tag="PLH85_00585"
     CDS             complement(25114..26001)
                     /gene="cysW"
                     /locus_tag="PLH85_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNB63083.1"
                     /translation="MSGAAAALHGVFDHAARFESRSATREPAWVKWTLIGLALAFFAV
                     FLLLPLVAVFYEALRKGWAVYYAALIEPDAVSAITLTLTAALCAVPLNLVFGVAAAWA
                     ITKFEFRGKHLLITLIDLPFSVSPVVSGLIYVLVFGAQGWFGPWLSDHDVKVIFAIPG
                     IVLATVFVTFPFVARELIPLMEAQGREEEEAAVVLGARGWQTFWHVTLPNVKWGLLYG
                     VILCNARAMGEFGAVSVVSGHIRGQTNTLPLHVEILYNEYQFAAAFAVASLLALLALV
                     TLVIKTWVERQAGRANKEE"
     gene            complement(26001..26837)
                     /gene="cysT"
                     /locus_tag="PLH85_00590"
     CDS             complement(26001..26837)
                     /gene="cysT"
                     /locus_tag="PLH85_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNB63084.1"
                     /translation="MSAARSLRVLPGFGLSLGYTVAYLSLIVLIPLAAVFLKAGSLGF
                     EHFWQAATSPRVLASYRLSFGASLIAALINAVFGLLLAWVLVRYAFPGKRLVDALVDL
                     PFALPTAVAGIALTALYAKNGWVGQILEPWGIQVAFTPLGVLVALIFIGLPFVVRTVQ
                     PILEDLDTEYEEAAVSLGANRWQAFLHVVLPTVFPALLTGFAMAFARAVGEYGSVIFI
                     AGNIPLVSEITPLMIITKLEQYDYAGATAIAVVMLIFSFILLLIINGLQAWTAQRTGR
                     AR"
     gene            complement(26834..27808)
                     /locus_tag="PLH85_00595"
     CDS             complement(26834..27808)
                     /locus_tag="PLH85_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB63085.1"
                     /translation="MASSALLGVTAAHADTTLLNVSYDVSRELYKDINPAFAAQWQRQ
                     TGEKLTINQSHGGSSKQVQSVVAGLEADVVTMNQAPDIDVLARAGLVAGDWRKAYPHG
                     ATPYTTTTIFLVRKGNPKGIRDWSDLTRPGLQVIVPNPKTSGNGRYTYLAAWGYALQK
                     TGNEAGARDFVSRLFANVPVLDGGGRGATTTFTQRDVGDVLVTFENEATLIDQEVGGD
                     KFDVVYPSVSIEAAAPVAVVRKVVDKRGSRKAAEAYLNFLFSPEGQDIIARHHFRPRD
                     PQVLKHYARRFPSIRTFTVEDALGGWDAVQKVHFADGGVYDQVGVKHR"
     gene            complement(28235..30832)
                     /gene="acnB"
                     /locus_tag="PLH85_00600"
     CDS             complement(28235..30832)
                     /gene="acnB"
                     /locus_tag="PLH85_00600"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNB63086.1"
                     /translation="MLEAYRQHVAERAALGIPPLPLSKKQAQDLVALLQNPPAGEEAF
                     LVELLTYRVPAGVDDAAKVKAEFLAKVAKGELACGLVSRAKATELLGTMLGGYNVKPL
                     IDLLADAEVGAVAAEGLKKTLLVFDYFHDVAELGKAGNANAKAVMQSWADAEWFTSRP
                     EVPAVQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGIEADEPGSRG
                     PIKQLEALAAKGNLIAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGS
                     KIAPIFFNTMEDAGALPIEIDAGQMDMGDEIELKIDAASGKVTATKNGATIAESQLKT
                     LVILDEVRAGGRIPLIIGRGLTSKAREALGLPVSTLFRLPQQPNDPGTGYSLAQKMVG
                     RACGLPEGKGILPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHT
                     AAYPKLVDVKMHRELPTFIANRGGVALRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFP
                     IGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVNAIPLWAIKAG
                     LLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELSDAAAERSAAACAIALNKEPIVEY
                     MRSNITLMKWMIAEGYEDPRTLKRRIAAMEDWIANGVLLKGDANAQYAAVIEIDLAEV
                     TEPILACPNDPDDVKVLSEVAGDKIDEVFIGSCMTNIGHFRAAGKVLDGKSDIPARLW
                     IAPPTKMDALILTEEGYYSVLGKAGARMEMPGCSLCMGNQAQIRKGSTAISTSTRNFP
                     NRLGIDTRVYLGSAELAAMCALAGRIVTKEEYMEQIKLVNAKAADVYRYMNFDQIPAF
                     VEVAETVEM"
     gene            complement(31148..31537)
                     /locus_tag="PLH85_00605"
     CDS             complement(31148..31537)
                     /locus_tag="PLH85_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB63087.1"
                     /translation="MSSPLTFQDKHVLIVDDNPINRTLAAAFTKRLGITSDQAADGAT
                     ALALIAANRYDAVLLDISMPGMSGEEVLARIRSDAVFSSLPVIAYTAHALPDEKQGLL
                     AAGFDDLLIKPVTMGAMEAVLASVLTG"
     gene            complement(31534..34104)
                     /locus_tag="PLH85_00610"
     CDS             complement(31534..34104)
                     /locus_tag="PLH85_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB63088.1"
                     /translation="MQTNTGRFGIRGKLISIFIVIKVVPLVLLALLAWTMAEKLGDAV
                     GLQLESVSSSMLRTLRSVGEEATQDALHALDDRARESMERLTTDTARAVAAFLYDRDA
                     DIREAAELEPGDASYRRFLAHRQRPVFSHGPWKLADDGKRWEPAAPESWDESLAADAG
                     QALSDNAKAFSARPPEFLGHREMRPLFVEMSFIDLSGRERVKVTQGSLMTPGLRQVSD
                     ARETFIRAEHYWPELQKLKPGEIHVSDVVGAYVKSHVIGPYLPASAEKAGEPFKPEAS
                     AYAGTENPVGRHFRGIVRWAMPVVRQGQITGYVTLALDHDHIRQFTDRISPTDARYTP
                     INDAIAGNYAFMWDHRGRAISHPRDYFIPGYDPATGEPVTPWMDQSLYEAWQSSGKSS
                     QAFLEGVKPYDDQSLKKKPAGALVKAGTVALDCRYLNFSPQCSGWKLLTEKGGSGSFV
                     IFFSGLWKLTTAAAIPYYTGPYAASPQGFGIVTIGANVDDFHRAATESAKRIGLTIDA
                     KDKEFQTLRAGISQAINDNLTQTAGALAISTLVMVAVVIAVAIWMAQFITRRITRMIE
                     GLDGFQAGDLSRRLEVKSTDEMGHLAESFNSMADRVQESFKRSEDARQSAEAANRMKS
                     EFLANISHELRTPLNGILGFAELLKLDATDAAHQEYAATIESSGQHLLDLVNDLLDLA
                     KVEAGRMELNLSDVLLATFAQEVCAAHRGHAQAKGLQLECTIAAGTPERLHTDPLRLR
                     QILNNLLNNAIKFTDNGRIDLNIHADGERIALAVSDTGRGIPKDAQDVIFDKFRQVDA
                     FVTREQAGTGLGLALARQFVHLLGGELSLRSEPGVGSTFTLLLPRDAATTARPTASPR
                     "
     gene            34281..34661
                     /locus_tag="PLH85_00615"
     CDS             34281..34661
                     /locus_tag="PLH85_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNB63089.1"
                     /translation="MSDPCPCGSGRAYPACCGPYLDQSLPAPTAEALMRARYTAYTLK
                     REDYLLATWDPAHRPAQLDLASDTATRWLGLQVKRHETTGSGQAIVEFVARYKVAGRA
                     YRLHETSRFHVLGGAWVYVDGDVH"
     gene            complement(34674..34889)
                     /locus_tag="PLH85_00620"
     CDS             complement(34674..34889)
                     /locus_tag="PLH85_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNB63090.1"
                     /translation="MATRFPVQGDFIQLDQLLKATGLCDSGGVAKQRVAEGQVKVDGV
                     IETRKRAKLRPGQRVEFAGEVIELVAT"
     gene            complement(34977..35465)
                     /gene="lysM"
                     /locus_tag="PLH85_00625"
     CDS             complement(34977..35465)
                     /gene="lysM"
                     /locus_tag="PLH85_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNB63091.1"
                     /translation="MGLLSFVREAGEKLFGRKDVEAAAADSGAAERLADLNAKAAAAI
                     LAYIGTQGLDASALSVSFDGATSTVTASGSVSDQATKEKILLCCGNVDGVEQVIDQLA
                     VTHPEPEAQFHTVVRGDTLSAIAKHYYGNANAYMKIFEANRPMLGHPDKIYPGQMLRI
                     PT"
     gene            complement(35560..35892)
                     /locus_tag="PLH85_00630"
     CDS             complement(35560..35892)
                     /locus_tag="PLH85_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB63092.1"
                     /translation="MSHPCLSCGACCASFRVDFHVSDLASHPGGGVPDALTVPVTATL
                     VRMRGTDDGPPRCVALSGEVGREVSCSIYEQRPGPCRDFAPYAALGIGDEGCARARRR
                     HGLPPLGA"
     gene            complement(35899..36597)
                     /locus_tag="PLH85_00635"
     CDS             complement(35899..36597)
                     /locus_tag="PLH85_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNB63093.1"
                     /translation="MNPIPVSMPLTEEELEQLEELLASDDVPADCMNMEMLDGYLAAV
                     VAAPGPIPVEVWLPAVWTADGDEAGFGSGAVLQKAIELVLRYHNDLLLAIGDDEADDA
                     WQPFCYAQGGDDDPAIGEEWTAGFEQGFELWPTEWTDTVEDTSIAEALLETLLGDDEE
                     DAVADSAEQLDILEAKGIAVHRLVQTWRALGLAAPEPVVVAAAAGAAPAGPGRNEPCP
                     CGSGKKYKKCCGAT"
     gene            complement(36594..37280)
                     /locus_tag="PLH85_00640"
     CDS             complement(36594..37280)
                     /locus_tag="PLH85_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB63094.1"
                     /translation="MYANSRIPESAQSGLHEALESRVNKHAATDFRKPYAPYNVAAFE
                     ASLAGWDRRAPLLLDAGCGVGHSTIALARAFPDHYVIGVDQSEDRLSRKKPYPDALMP
                     TNLVFCRADLVDYWRLLADAGIRLDRHYILYPNPWPKIGHLTRRWHAHPVFPCVPRLG
                     GRLECRSNWPIYIAEFARAVELLTGVPAPWQPLEAPSPLTPFERKYRDSGQTLYQCVV
                     ELDQLEEQSS"
     gene            complement(37290..38273)
                     /locus_tag="PLH85_00645"
     CDS             complement(37290..38273)
                     /locus_tag="PLH85_00645"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNB63095.1"
                     /translation="MPRLYLAPMEGLADDVLREVLTRIPGYDHAVTEFLRVSATVLPG
                     RSFTRIAPELKVGSRTSSGTPVHLQLLGSDPQRMAASAARLVRLHPAGIDLNFGCPAP
                     TVNRHRGGAALLDEPELLHAIACAVRASVPKGIPFTAKMRLGIADTGRTLDCARALAD
                     GGIDALVVHARTKVDGYRPPAHWEWVGRIADTVGVPVAANGEVWSEADWQRCRAVSGV
                     SDVMLGRGAVADPFLARRIRALQADGTKTPDRDAEWAELSPLLGEFWWRVRAKLEARH
                     APGRLKQWLNLLRRNFPQAESLYGEIRALKDCTAVEGHLRRHGVPVLSAAA"
     gene            38446..39783
                     /locus_tag="PLH85_00650"
     CDS             38446..39783
                     /locus_tag="PLH85_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNB63096.1"
                     /translation="MRRIDFTNRREWLTLALPALLLVVAAFAVAWKFVQPAPPRQIVM
                     STGPAGGAYQYFAERYRERLARDGIELALRPSTGAVENLNRLKTVDEVDMGFVQGGVA
                     DEPESDDLATLGSMYLEPVWVFFRGKADLDRIDQLKGRRIAVGAPGSGTLLFALQLLS
                     ANGFATDDPHFIHLGGLEAVEAIRSGQVDAVFAVGAPQAPAIQALLAEPGLSLLNFAQ
                     ADGYVRHFPHLSRITLPRGAIDIAQDRPPRDIHLLAATANLVVMADLHPAIVTRVLQH
                     AAQIHGQPGMFMTPFAFPANQDRSLPLHPSARRFYESGPPFLQRYLPFWLAVLIDRLI
                     VMLVPIVAVVIPLFKVLPALYNWRMRSRVYRWYGELKFLENEIGQQSTHTPEVLDDFI
                     RRLDGIEDRASRRKLPLAFSNELYTLREHINLVRQRLERLQARAQVPAAPTVS"
     gene            39839..40450
                     /locus_tag="PLH85_00655"
     CDS             39839..40450
                     /locus_tag="PLH85_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB63097.1"
                     /translation="MSPHLDLTEPYAPLVRRIEAEATVTTDGNLRIDHFLNHRVEPAY
                     IAELGHELARRLSYFQPNVILTAEASGIAPALIVAQVLSIPMVYAKKYSCDVEGPAIS
                     RIVASPGRGAEVRLALSSRYVRSGHRVVVVDDFLSNGRTAAALVEMAREAGAEVLGAG
                     FIVEKRFNRGREHIAAMNVPIATLAQIEFLHDGRACLTQPPAA"
     gene            40459..41754
                     /locus_tag="PLH85_00660"
     CDS             40459..41754
                     /locus_tag="PLH85_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNB63098.1"
                     /translation="MSPRQILRSLFDAALAAAQPERVVPLHLPDGGALPRGRVIVIGA
                     GKASAAMARAVEDHWPGDPARLSGLVITRYGHGVPCRHIEIVEAAHPVPDERGYAAAR
                     RMLEHTVAELGPDDLVLCLISGGGSALLAVPAAGITLADKQAIGRGLLASGATIGEIN
                     CVRKHLSAIKGGRLALACQPARVLTLLISDVPGDDPSIIASGPTVGDPTTCAEALTVL
                     DKYAIPLPAHVRAHLESARGESPKPGHPAFARCETRLIATPQQALEAAARRARELGLS
                     AHILSDAMEGESRHVARVHAALARQVADRGQPFVAPCVLLSGGETTVTVSGKGQGGRN
                     VEFLVALAQALDGHPRVHALAADTDGVDGSLDIAGACIDPTTPARTLALGRRLADVLA
                     DNDGHGLFQALGDSVVTGPTQTNVNDFRAILIDAPDAAL"
     gene            complement(41783..42685)
                     /locus_tag="PLH85_00665"
     CDS             complement(41783..42685)
                     /locus_tag="PLH85_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63099.1"
                     /translation="MSSFSTRWRAEMCRGSSRWRAGVLMGALLVSGARAECPADVEAA
                     AVAAAIVNRTPVHAVPGLTRMEDGLCGRDKVVRFLAQAWGAPVGHKAGLTNPAVQQRF
                     GYGAPLRGRLFRAGLLPDGAQVPAAFGIRPVFEADLLVEVADPHALARAGSPDAALQA
                     LSAVIPFVELPDLMVGDPGALSGPMLALVNVGARLGVQGTPVPARSGGLRAEALASMT
                     VSVRDGTGAVLDSAQGSAILGHPLNAAIWLARDLAAAGTPLQRGDLLSLGSFSRLLAP
                     KSGLDIVVRYDGLPGNPEVRVHFR"
     gene            complement(42654..43469)
                     /locus_tag="PLH85_00670"
     CDS             complement(42654..43469)
                     /locus_tag="PLH85_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNB63100.1"
                     /translation="MAHGLLKFFRSVLLGLAVSTAAVQAGESIVVASTTSTEQSGLFN
                     FILPIFEKDSGIAVRVVALGTGQALDVGRRGDADVVLVHDRPAEDKFVADGYGVDRRD
                     VMYNDFVVIGPAADPAGVKRARDASDAFARIGSQGATWISRGDRSGTHAAELRFWKAA
                     GVDPRGQGWYKEAGAGMGPTLNMAAGLGAYTLSDRGTWASFRNRQDLGILFAGDPQLY
                     NPYGVILVNPARHPHVKRAAALRFMDWLTSADGRRAIAAYKIGNEQLFYPLAR"
     gene            complement(43451..44164)
                     /locus_tag="PLH85_00675"
     CDS             complement(43451..44164)
                     /locus_tag="PLH85_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB63101.1"
                     /translation="MFALTVSDLVWRAGPRTILDGLNLRLDGEGITVLLGPNGAGKSV
                     LLRHVCGLLRPDAGLIDWNGAATPGAGVSMVFQQPRMFRDSILGNVALALTPRGIAAT
                     ERRRRAAAVLERVGLAHRLTDSARGLSGGERQRLALARAWVTRPQLLLLDEPTASLDP
                     SSVEAIERIVREIRTDGTRILMTTHNLGQATRLADDVVFMDAGRVCEHAPVQRFFARP
                     ASPAARLYIQGELPWRMAF"
     gene            complement(44177..44881)
                     /locus_tag="PLH85_00680"
     CDS             complement(44177..44881)
                     /locus_tag="PLH85_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63102.1"
                     /translation="MSGMGQAFLEAFQLLTGFDRQVVDIVVLSLRVSGLAVLAGTLVG
                     LPLGACLGVGRFPGRQALVIFLNACMGLPSVVVGVVVYLALSRSGPFGHLGLMYSPVA
                     MVIAQTLLVIPLMAAIARQVVEDAWRDYAEELAAMRFRWIDSVTLLLHDCRHSLLVAV
                     LAGLGRAMSEVGAVMIVGGNIDRSTRVMTTAIALETSKGDLPLALALGVVLLAVILLI
                     NAAAYMLRTWAFRRYA"
     gene            complement(44898..45203)
                     /locus_tag="PLH85_00685"
     CDS             complement(44898..45203)
                     /locus_tag="PLH85_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63103.1"
                     /translation="MVENVLAPEGFSREMSISMTEFERALRLAETDRTITVTAGRFRV
                     EGDGAVLDVSLTPLPDRVIGMIRLPALAAHLRFLAGNGAERRTLLHRIDLAMRRGGG"
     gene            complement(45212..45463)
                     /locus_tag="PLH85_00690"
     CDS             complement(45212..45463)
                     /locus_tag="PLH85_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB63104.1"
                     /translation="MKIAFKLFASLADYLPAERRSNRVELDLAPGTTVGDLIDRYRIP
                     ERSAHLVLVNGHYIEPNARRSHVLAEGDELAIWPPIAGG"
     gene            complement(45460..46746)
                     /locus_tag="PLH85_00695"
     CDS             complement(45460..46746)
                     /locus_tag="PLH85_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB63105.1"
                     /translation="MKHLILGNGPAGVVAVEALRKADPRGEIVMVGSENEPPYSRMAI
                     PYLLEGNIEEPGTWLRKTPGHFDALGVRQLSGRAVALDVADRRVLFADGHFEHYDRLL
                     VATGSHPVRPPIPGVDLPEVQTCWTLEDARAIARLAKPGSRVLQLGAGFIGCIIMEAL
                     AKRGVQLTVVEMGDRMVPRMMTPQAGGMIKRWVENQGIRVVTSAGVERIDHGDGHGAP
                     LLVSLSTGDKLPCDLVIVAAGVAPNVSFLEATPVHVAKGVLVDARMETSVPGIFAAGD
                     VAEAPDLFTGAHLVSAIQPNAADQARVAAINMAGGQARLTGVLAINVLDTLGLISTSF
                     GQWWGDPEGQGVEQVDEVAFRYLSLQFKEDVLIGATSIGMTQHVGALRGMIQGRLRLG
                     PWKDVLLHAPQRFFEAYLAATQSDAIARHQPVAAFA"
     gene            complement(46873..48720)
                     /locus_tag="PLH85_00700"
     CDS             complement(46873..48720)
                     /locus_tag="PLH85_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNB63106.1"
                     /translation="MGWNRKILRVNLTAGTCKEEPLNMAWADEYLGSRGLATKYLVSE
                     IDPKVDPLSPENKMIMSTGPLTGTVASTGGRYTVVTKGPLTNAIACSNSGGFFGAEMK
                     FAGWDMIIFEGRSPKPVYLYVENDKAELRDASHLWGKSCWETEEMIKHAHQDPLVRVS
                     SIGLAGENQVLFSCIVNDLHRAAGRSGVGAVMGSKNLKAVALRGTQGVSGIKDMKAFL
                     KITAEKKKILADNAVTGQGLPKYGTQVLMNVINEIGALPTRNHRNVQFEDAGKISAEA
                     MHEKRPTDGKPHLVTNAACFGCTIACGRISEIDKGHFTVVNNPKYWGASGGLEYEAAW
                     ALGAANGVGDLEALQYANLLCNEQGMDPISFGATIGAVMELFEMGLLTKEQLGVEAPF
                     GSAAALAHLAEITAKGEGFGKEIGLGSKRLCTKYGHPELSMTVKGQEFPAYDARGIQG
                     MGLTYATSNRGACHLRSYTVASEVLGIPVKTDPLTTEGKPELVKAFQDATAVFDASGI
                     CVFTSFAWTVADVQPQVQAACEGDWSMDKLNAMGERIWNMERMFNLAAGLTHKDDDLP
                     PRLKTEAADSGPAKGLVSGIDKMLPEYYALRGWSEEGVPTASTLERLGL"
     gene            complement(48736..49152)
                     /locus_tag="PLH85_00705"
     CDS             complement(48736..49152)
                     /locus_tag="PLH85_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB63107.1"
                     /translation="MACSVEHTGIINPSKSRIKVFSFETEGRKVPYTCTQCTEAWCLN
                     ACPVDAIRVNGTTGAKEVHEDVCVGCKVCTISCPFGTVNYNQETGKVQKCDLCGGDPA
                     CASACPTGAITYIDADWTGLDRMRAWAAKANTAVTE"
     gene            49498..50754
                     /locus_tag="PLH85_00710"
     CDS             49498..50754
                     /locus_tag="PLH85_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNB63108.1"
                     /translation="MSQDSGCAAPSSSPASLTVDAARRLIFDRLTPVGGYERIALRAA
                     LGRILAEPVIAPCNVPAHDNSAMDGYAIRADDLPESGEASFQVVGTAYAGRGFSGIVG
                     AGQAVRIMTGAVVPMGADTIVVQEIVQTDGDRVVVPPGQRRGQNLRRAGEDLALGAIA
                     LPAGKRLGPAELGLVASLGIAEVSVTRRLRVAFFSTGDELASIGRPLAPGEVYDSNRY
                     TLFGALTRLGCDLLDMGVIRDEPESMETAFREAARHADVIITTGGVSVGEADFIRDLM
                     ARLGEVSFWKLEIKPGRPMAFGRLGSAWMFGLPGNPVAVMVTFYQFVQDALLHLGGLT
                     PLPERPLVPAECADAIRKQPGRREFLRGRLSREDGIWKVRLSGAQGSGILRSMSEANC
                     FIVLEETRGNVAPGDAVSVQLFDGLI"
     gene            complement(50858..52786)
                     /locus_tag="PLH85_00715"
     CDS             complement(50858..52786)
                     /locus_tag="PLH85_00715"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HNB63109.1"
                     /translation="MLSRYEQRERKPMASYKEFHKRSIEDRDGFWGEQAQLIHWNKQP
                     SQIYDFSNPPFVKWFVGGETNLCYNAVDRHAESHPDRKALVYISTETDEEKVYSFAEL
                     RTEVMRMAAIYQDLGVKKGDRVLIYMPMIAEACFAMLACTRIGAIHSVVFGGFASHSL
                     ATRIDDAKPTVIVSADAGMRGGRPVPYKHLLDEAINLAEHKPAKVLMVNRNLDPNMAV
                     VAGRDVDYAALREKHLDAQVPVTWMESSEPSYILYTSGTTGKPKGVQRDTGGYAVALA
                     ASMKHIYCGNPGETFFSTSDIGWVVGHSYIIYGPLINGMATIMYEGTPLRPDAGIWWK
                     ICQEQDVTVMFSAPTAIRVLKKQDPAFLKKYDLSKLRALYCAGEPMDETSHQWMMDEL
                     GIQVIDHYWQTETGWAMLSLMPGVERQEVQLGSPGFPVYGYDVRLFRDDGSECGPNEK
                     GIVGVVPPLPPGCLTTVWGDDKRFVSTYFSLFQEPLVYSSFDWGIKDDAGYYKILGRT
                     DDVINVAGHRLGTREIEEAIQSHSAIAEVAVVGVADQLKGQMPMAFAVVKDPASVDTP
                     EKVVELEKAVMKIVDGSLGAIARPARVHFISGLPKTRSGKMLRRSIQALAEGRDPGDL
                     TTIEDPSTLEQIKSALTK"
     gene            complement(52823..54007)
                     /locus_tag="PLH85_00720"
     CDS             complement(52823..54007)
                     /locus_tag="PLH85_00720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB63110.1"
                     /translation="MNDDSDQALPRVLIIDDSRMVRASLIRHLRGRFEVREEADGEDG
                     WQTLMVDPTIQAVITDIGMPRLDGYGLLERIRGSRLSRISTLPVAVISGDDEPEVRVK
                     AKSAGATDFISKSISTVELLARLEALTQLARTQRDLEESRAALATSSPVDPVSGLATP
                     SYLHHHAAQELSLAARRHTDVSVMVIEIDGFDTLVSRYGAHVAQLIHRKLSRILATKL
                     RQEDTVAELATARFAVVSPNTDIDGVAAFAFRLRQAINHMVMTYREERIHIHLTVGIA
                     ANSPDGRTTVSHLIADALQRLSTGRTLGGDRVIGPHGEVTEASVAQKAAARPPVSIDA
                     LLQRVRAGNATDVRGQLPDAIRTLLPLLELIESEFRSGLPLASLARAADTGLVAGFHN
                     NG"
     gene            complement(54033..55043)
                     /locus_tag="PLH85_00725"
     CDS             complement(54033..55043)
                     /locus_tag="PLH85_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNB63111.1"
                     /translation="MNTPLPADFQAFVIHQDAQRKVDSRMERMTVDQLDAGEVLIRVA
                     YSSVNYKDALAATGAGKIIRRFPCVGGIDLSGTVVSSADSRFKAGDPVIATSFDIGVA
                     HHGGYAEYARVPAGWVVPLPAGLSLFDAMALGTAGFTAALGIVRMEDNGLCPDNGPVI
                     VTGATGGVGGLAIDMLAGLGYHVVALTGKAEEEAYLRELGAAEIRLRSTIDPAKTRPL
                     DAGLWAGAVDNVGGDILAWVLSTMKQAGTVASIGNAQSISLNTTVFPFILRGVSLLGV
                     DSGYMGFPTRTRVWDRLATDLRPRHLESMSRTVPFADLPGVFDDFIAGRVKGRTVIAV
                     SA"
     gene            55196..56095
                     /locus_tag="PLH85_00730"
     CDS             55196..56095
                     /locus_tag="PLH85_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB63112.1"
                     /translation="MALITRPFETLSAYEAFFAHEEMPVLRHTVKELDAMREQEDHVN
                     GRAVAALVLSDPMMTLRVLIHLEQNRRASQNHDITTVERAIMMMGISPFLRRFADLPT
                     IEAQLHDHPKALIGVLRVISRARRAAKYARDWAIVRHDLDVDEITVAALLHEVAEIVC
                     WCFAPTLTDEVYAMQRADHSLRSVAAQRRVFNVSAQDLQSCLVRQWHLPELLVSMMDT
                     HQINNPRVATVKLAGNLARHTAQGWDNAAIPDDLAAIEALLHVGRETLLHRLAVPHED
                     ADRLLNAGGQAAGALPPEQSPQA"
     gene            complement(56086..57999)
                     /locus_tag="PLH85_00735"
     CDS             complement(56086..57999)
                     /locus_tag="PLH85_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB63113.1"
                     /translation="MPLLTLDDACLAFGHVALLDKADFQLDAGERVALIGRNGSGKSS
                     LLKALAGQATLDDGRVWRQPGVRIAYVPQEADFSLDLSVFAAVAEGLGDVAQLLVDYH
                     AAMLAVADEASDAALAHLEAVQHRVEDAQAWRLEQRVEQILGTLRLAGDAPVGSLSGG
                     GIKRVALARALVGEPEVLLLDEPTNHLDIDGILWLEALIREFPGAVVVITHDRVFLDH
                     VATRIVELDRGLLSSFPGRYADYQVRKADQLDAEEKANARFDKLLAQEEVWIRKGVEA
                     RRTRNEGRVRRLEALRRERGERRDRLGNVRLAVDRGESSGQMVAELEDVGKRFGERWV
                     ARHFSTRILRGDRIGLIGPNGAGKTTLLKLILGEIEPDEGRVRRGTRQTVAYFDQLRA
                     QLDPEAPLTEVISPGSDYVEIGGHRTHVIGYLGDFLFSPARARSPVKSLSGGERNRLL
                     LARLFARPANVMVLDEPTNDLDIETLDLLEDLLARYDGTVFLVSHDRAFLDNVVTQVI
                     AAEGEGDWKEYAGGYEDWLRTRPAAVPAVAPVVPATPKTAPPPRKEAAPDRPAKLSWK
                     EQRELEGLPDRIAALETQQADVQARLGDPTLYKDAPQEVPVLKAQLEALEKDIDAALS
                     RWEALESRAAAQA"
     gene            58203..58388
                     /locus_tag="PLH85_00740"
     CDS             58203..58388
                     /locus_tag="PLH85_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HNB63114.1"
                     /translation="MSPALSPSSQTTNDDARRESASPVTLRSADLLQDRGCVLIEHQG
                     MVYALRATRAGKLILTK"
     gene            58385..58528
                     /locus_tag="PLH85_00745"
     CDS             58385..58528
                     /locus_tag="PLH85_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63115.1"
                     /translation="MIFVAPSRQPGAFSTRPMSLLGVAALLATGLAGSLGSLFLVLHF
                     YAS"
     gene            58548..58784
                     /locus_tag="PLH85_00750"
     CDS             58548..58784
                     /locus_tag="PLH85_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNB63116.1"
                     /translation="MYVCVCRAITERHITEAVNDGARRLRDLRRDLGVTEECGRCARC
                     AHACLKEAIDQIGPVSILSGCRTESTYSLSQEAA"
     gene            58781..59257
                     /gene="bfr"
                     /locus_tag="PLH85_00755"
     CDS             58781..59257
                     /gene="bfr"
                     /locus_tag="PLH85_00755"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNB63117.1"
                     /translation="MKGDKKVLQHLNKQLTIELTAINQYFLHARMYKNWGLGKLGKHE
                     YEESIEEMKHADALIERILFLEGLPNLQNLNKLLIGENVPECLQGDLKLELGGQAELK
                     EAIAYCETVRDYVSRELFESILEDTEEHIDYLETQIELIDKVGLQNYLQSQMDVPS"
     gene            complement(59354..59860)
                     /locus_tag="PLH85_00760"
     CDS             complement(59354..59860)
                     /locus_tag="PLH85_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HNB63118.1"
                     /translation="MMGDERDVLRPEPLTSAAFAPFGEVIEAASARQRILINEGTAER
                     FHDLARIEPGADGRAIVSIFRGQPRPMPFPVSMLERHPLGSQAFVPLSGRPYLVVVAH
                     GRDTPDEARLRVFLAADGQGVNFAPGVWHHPLLTLDEVCDFLVIDREGPGLNCEEKSL
                     TRPVHVAP"
     gene            complement(59857..60897)
                     /gene="alc"
                     /locus_tag="PLH85_00765"
     CDS             complement(59857..60897)
                     /gene="alc"
                     /locus_tag="PLH85_00765"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HNB63119.1"
                     /translation="MTTHSFDPPRIAAPAVLPDWARRSVNLADSRIGARALFATDEFF
                     APLERMLNPEPAVFVPGQYDANGKWMDGWETRRKRTTGYDWSLVRLGRRGIVRGFDID
                     TSHFTGNFAPAVSIDATDCESDEPDLLVRARWTRILPSTSLTGNSHHLLEIESSDTWS
                     HLRINIYPDGGIARLRVYGQPVGVFDTSDPEREYDLVALENGGRAVAWNDAHFGSAAN
                     LIAPGRGINMGDGWETRRRREPGNDWCILQLGAPGVVDRIEVDTAHFKGNFPDRCSIQ
                     AARMDGGTDQSIITQSMFWPVLLPEQPLGMDALHVFREGIAGLGPVTHVRFNIIPDGG
                     VSRLRLWGRVTR"
     gene            complement(60894..61835)
                     /gene="puuE"
                     /locus_tag="PLH85_00770"
     CDS             complement(60894..61835)
                     /gene="puuE"
                     /locus_tag="PLH85_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HNB63120.1"
                     /translation="MSPIHNSEYPRDLVGYGAHPPHADWPGRARVAVQFVLNYEEGGE
                     NCVLHGDPGSEQFLSEMFNPAAYPDRHLSMESIYEYGSRVGVWRILREFERRGLPLTV
                     FGVGMALQRHPELTAAFVALGHEIACHGWRWIHYQNVDEATERAHMAQALDVIEDMTG
                     TRPLGWYTGRDSPNTRRLVADDGRLLYDSDYYGDDLPFWVQVTRSDGVRVPHLIVPYT
                     LDTNDMRFALPQGFSHGDPFFQYLRDSFDVLYAEGDERPAMLSIGMHCRLLGRPGRFL
                     ALQRFLDHLARHDRVWICRRIDIARHWIAHHPWRAPA"
     gene            61886..62401
                     /gene="gpt"
                     /locus_tag="PLH85_00775"
     CDS             61886..62401
                     /gene="gpt"
                     /locus_tag="PLH85_00775"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:NF006613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNB63121.1"
                     /translation="MERSPNSPYRDDIVISWPELHRDTRLLCHQLMEKGPFKGIIAIA
                     RGGLIPAALVARELEIRLIDTICAASYEADNDDTAQTVRREVKILKGIEHDGDGYLLI
                     DDLVDTGNTARVIRAQLPKAYFATLYAKPMGRQVVDLCIKEFSQDKWIYFPWDIDYQF
                     VPPIKKHHRPA"
     gene            complement(62402..63589)
                     /locus_tag="PLH85_00780"
     CDS             complement(62402..63589)
                     /locus_tag="PLH85_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNB63122.1"
                     /translation="MDAAYLIDVGSLLLRWLHLIVGIAWIGSSFYFVWLDNSLKAPTD
                     PELIRKGVGGELWAVHGGGFYNPQKYLVAPQHLPEELHWFKWESYSTWITGFFLLGVL
                     YYSQAATYMVDPAKAALSPAAAIGIGLATLAGGWLVYDGLCRLLINRSLLLFAALYFA
                     FVVAVAWGLTHLLSGRAAFLHVGAMIATTMSGNVFFWIIPGQKTTVAAMQRGETPDPI
                     HGKRAKQRSVHNNYLTLPVLFCMISNHYAFTYNHPQAWRVLAMILLAAVLIRHFFNLR
                     HKGQVAWQYPALGAALLLAVFVELAPRHAAPAAGSPAASLADIQPIVVARCTGCHAAR
                     PTLIPAAPAGVLLDSAEAIRANGPRILAQAVQLKAMPLGNVTGMTDSERERIAAWAAG
                     GYR"
     gene            complement(63589..63942)
                     /gene="uraH"
                     /locus_tag="PLH85_00785"
     CDS             complement(63589..63942)
                     /gene="uraH"
                     /locus_tag="PLH85_00785"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNB63123.1"
                     /translation="MGRLTTHVLDTAAGVPAAGMHFQLYRLDGDCRTLLSAGQTNSDG
                     RSEAPLLSGETLLPGQYELAFAVAEYFRNRGVALPEPPFLDRVPLAFGIADPAGHYHV
                     PLLVSPWSYSTYRGS"
     gene            64164..64685
                     /gene="uraD"
                     /locus_tag="PLH85_00790"
     CDS             64164..64685
                     /gene="uraD"
                     /locus_tag="PLH85_00790"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HNB63124.1"
                     /translation="MPSRFTPAQLSDLDRADFIAAIGGVFEHSPWVMARAWEMRPFSS
                     RADLDAALVKVIREAGRDELLALIEAHPELAGKAAIRGDLTADSAREQAGAGLNACSP
                     EEFELIQRLNRAYRARFGWPFIVAVKGMTRHDIIAAIERRLPQTSTEEFVEALAQILR
                     IAGFRLDELLTDA"
     gene            complement(64731..65717)
                     /locus_tag="PLH85_00795"
     CDS             complement(64731..65717)
                     /locus_tag="PLH85_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB63125.1"
                     /translation="MATTCSWESIDVHLLQVLHALLTECSVSNAARRLNQSQPAVSTA
                     LRRLRDITGDQLLVRSRNGMTPTERGMALLEPVKVALAQIEAIGLQQIRFDPMKSRRT
                     FNIATPDYLNAILIGNIVARLRRLAPNSQVVLHSFGPDYDYAKALESGELDTVIGNWP
                     QPPEHLRLAPLFDDDVVCLMRRSHPLAGRTLSVDDYLNAEHLVPTPYAVGQRGVIDMQ
                     LARERLKRNVVASVPYFNLVPYVLLQNDIIFSAPRIFAEYCCSLGELCFSESPLDFPK
                     MRFYLLWHDRAHHSEECRWFRDQIIEVIREKTGAPAAAGTRVPAQVATPATV"
     gene            complement(65987..66826)
                     /locus_tag="PLH85_00800"
     CDS             complement(65987..66826)
                     /locus_tag="PLH85_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63126.1"
                     /translation="MLVKKTALGCAMLIAGLSCAHAADWSDTYVGWRYGTKFAEPYNP
                     DDIKKNIFNLGHVSGYKYGTNFLNVDMLLSDSKDSFNNGGGAQETYVVYRHTFDFGKI
                     AGNPDAFKFGIVRGLGFTVGFDYNTKSGDSYQSRKRMPLAGPTVMFDVPGFLNLSVLQ
                     LWESNAPRTHPSRYSYDAHPMLTLAWGIPLGSLPLTFEGFMNYIAAKGANEFGGPTKP
                     ETNFDGQIMYDASPLVGARPKTFKVGVEYQYWRNKFGNDHTGAAGNGAFAKTPMLRAE
                     YHF"
     gene            67224..68252
                     /locus_tag="PLH85_00805"
     CDS             67224..68252
                     /locus_tag="PLH85_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB63127.1"
                     /translation="MPIPFNAGRRLVLAAAMSLLAMPAFADAPTRIKFTLDWRFEGPA
                     APFLLAKAKGYFAAEGVDMEIDSGSGSAGAVTRVATGAYEMGFADFNALVEYDAKTPG
                     SKIQGVYMVYNATPAAVFVLKKSGITKPADLAGKTLAAPIFDAGRKAWPAFAKSNKLA
                     VDAVKWQTVEPALRETLLARGDVDGITGFYFTSLLNLEARGVKSDDIVALKYPDFGVE
                     LYGNAIIASPKLMAENPKAVAGVLRAFNKALKEVIANPAAAIAYVKERDPLINADLET
                     RRLKLALDSSVVTPEVKLNGLGAVTPERMQRSIAETAEAYALPKVPTAADLFNPNFLP
                     SKTDRTLK"
     gene            68314..69093
                     /locus_tag="PLH85_00810"
     CDS             68314..69093
                     /locus_tag="PLH85_00810"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB63128.1"
                     /translation="MSFVTFEDVSLAYTPGGANAIENVSLSIREGEFVAVVGPSGCGK
                     STLMKLVSGLKPPSAGYVYVDGREVGGPLKIVGMAFQAANMLPWRTTLDNVMLPLEIV
                     EPYRSRLRRERETFKADAEALLARVGLAGFGQKYPWELSGGMQQRASICRALIHQPRL
                     LMLDEPFGALDAFTREELWCMLRDLWQQQPFTVVLVTHDLREAVFLADTVYVMSKRPG
                     RILIRKEIDLPRPRPLDVTYTDAFAGYVHELREHIGRIRES"
     gene            69105..69881
                     /locus_tag="PLH85_00815"
     CDS             69105..69881
                     /locus_tag="PLH85_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63129.1"
                     /translation="MSLKQSTLVKSAPWLLTAVLFIVWELICVGFAIPDYLLPAPTAI
                     WASGLENAAPIMMHATQTLMTTLAGFALAVVVGMVLGLAVGASPVVYKATYPLLVGFN
                     SIPKVAFVPVLVVWFGIGTIPAILTAFLISFFPVVVNVATGLATLEPELEDVLRSLGA
                     HRWDIMVKVGLPRSLPYFFASLKVAITLAFVGSVISETVASNLGIGYLMMSASSSMNM
                     PLVFAGLIVIGVMGVVMYELFALLERNLTGWAHRTPGAAT"
     gene            complement(69990..71315)
                     /gene="guaD"
                     /locus_tag="PLH85_00820"
     CDS             complement(69990..71315)
                     /gene="guaD"
                     /locus_tag="PLH85_00820"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNB63130.1"
                     /translation="MTHRTVPDRLILGQILHFLDDPGEGDDPHSYAHFPQGALWIVEG
                     RVAAVGHRRAVESAIAPERLAAAERFDYGDRLVLPGLVDTHCHYPQSAVIASFGRQLL
                     DWLNTYTFPAEMAFRDPEVARATARYFLDRLLAHGTTTASVFATVHAASVEAFMAEAD
                     ARQLRMLTGKVMMDRHAPPELCDTVADAERDCVALIERWHGRGRLRYTITPRFAPTST
                     SAQLALAGELYASRPDLHVQSHLAENPDEIAWVKALFPECRDYLDVYARHGLLGPRTI
                     YGHCIHLSGAERAEMAATGTAAAFCPTSNLFLGSGFFDHAASVEAGLRVGLATDVGGG
                     SSFSLIRTLDEAYKVSQVHRRPLSPLRAWYLATLAGARALYLDAFIGNFEVGKEADCV
                     VLDERATPELEFRLRGVTGLAERLFALTVLGDERCVVATHVLGAPAFQR"
     gene            complement(71325..72686)
                     /locus_tag="PLH85_00825"
     CDS             complement(71325..72686)
                     /locus_tag="PLH85_00825"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNB63131.1"
                     /translation="MATLLLKDAEVLVTQDATRREIPGGGVFIRDGVVEAVGPSDQLP
                     DGADEVISLAGCVALPGLVNTHHHMYQSLTRAIPGAQDAELFGWLRRLYPLWAGLTPE
                     MIRVSTLTAMAELILSGCTTSSDHLYLYPNGSRLDDAIEAAAILGMRFHAARGSMSVG
                     ESAGGLPPDRLVEDEAAILKDTQRLIETWHDPARHAMRRIVVAPCSPFSVSRELMREA
                     AILARGHGVSLHTHLAENDNDVAYSREKFGMSPAEYAESLGWVGADVWHAHCVKLDGP
                     GIELFARTGTGVAHCPCSNMRLASGIAPIPAMRAAGVPVGLGVDGCASNDAAHLLGET
                     RQAMLLARVGYGPSAMSARQALELATLGGARVLGRDDIGALAPGMSGDVVAFPTDELD
                     LAGAAVHDPVAALVFCTPPKVKVSVINGQPVVREGQLVPLELPPLIAHHNRLARQLAD
                     HRA"
     gene            complement(72692..73612)
                     /locus_tag="PLH85_00830"
     CDS             complement(72692..73612)
                     /locus_tag="PLH85_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63132.1"
                     /translation="MIEHLVPVLAGAIAAATPLIFAGLGELVAERTGVINLGVEGMML
                     TGAIAGFAAAAESGGGAAAGLPAGALAGMATALLFAVFALSLAANQAACGLALTIFGI
                     GLSAFIGQHYVSYSLPGLAPVDIPLLADIPVLGPVLFHQDAVVYLSFAAFAGVAWILA
                     RTRLGLLLKAVGESPEAAHAVGYPVIAIRYGAVVFGGAMAGLAGAYLSTVYTPLWVQN
                     MSAGRGWIAVALVVFATWKPLRLLLGAYLFGGVTILQFHAQGLGLDVPVQLLAALPYA
                     ATVIVLVFISRDRRLLQLNLPASLGKSFLP"
     gene            complement(73619..74677)
                     /locus_tag="PLH85_00835"
     CDS             complement(73619..74677)
                     /locus_tag="PLH85_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63133.1"
                     /translation="MHLLEPRAQPSQLMRWTAPLIAIAATLLTGSLLFWALDKSPLTS
                     FEVFFVQPLTSSYGWSELLIKACPLILIAQGLAIGFKARIYNIGAEGQLIVGALFGGG
                     VAIHFNGSTSAWVIPAMVVAGAIGGALWGALPALLKTRFNAEETLTTLMLTYVATFIL
                     SYMVNGPWRDPEGMNFPQSIMFDDNALFGILLEGSRVNSSVFITLVAVLAFWLFNARS
                     FLAYQLEVGGQAPAAARYAGFSASQTVWFSLAMSGLTAGLAGVGEVAGPVGQLNLNIS
                     PGYGYAAIIVAYLGRLHPLGIVLAGALMALIYLGGEAAQVSLQMPAAITGIFQGMLLF
                     FLLGADAFIDNRLRRPSR"
     gene            complement(74664..76205)
                     /locus_tag="PLH85_00840"
     CDS             complement(74664..76205)
                     /locus_tag="PLH85_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB63134.1"
                     /translation="MSTPEPRLQLLHITKRYPSVVANSDISLTVMPGEIHAILGENGA
                     GKSTLMKIIYGAVKADEGEVMWNGHQVAIANPAQARKLGIGMVFQHFSLFETLTVVEN
                     IALVLDEAFDLPALAKRVIEVSERYGLPLDPFRLVHSLSVGERQRVEIVRCLLQDPKL
                     LILDEPTSVLTPQAVLKLFDTLRTLAAEGVSILYISHKLHEIKALCDSATIMRGGKVT
                     GNATPRDETPASLARMMIGRELPVCEAPPFIGESRPVLQVSGLSLPSDDPFGTSLKDI
                     SLTVHAGEILGIAGISGNGQAELLAALSGETPVARDAICLEGAAIGHLDAGRRRDRGL
                     VFVPEERLGRGAVPSLSLARNALLTAHRQGMKRLGLVSYAKARAFAEQCIARFDVRCG
                     GAASAARSLSGGNLQKFIVGREIMLSPKVMIVAQPTWGVDVGAAAFLRQTLLDLSRTG
                     VAILVVSEELEELFEISDRIAVLAAGKLAEPEPKAATNAEKVGLQMSGLFTAAQSGES
                     VHASA"
     gene            complement(76208..77221)
                     /gene="xdhC"
                     /locus_tag="PLH85_00845"
     CDS             complement(76208..77221)
                     /gene="xdhC"
                     /locus_tag="PLH85_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HNB63135.1"
                     /translation="MTTRAPDWLKALPAVLEAGQSVELVTVARADGSTPREAGAAMLV
                     GRETIVDTIGGGHLEWEAISHARRRLAVGGGRPEIVRYNLGARLGQCCGGVVWLVFES
                     MPASERDAWVERRASVLAGHHLHRQVDSEAAASVWQRAPVAGGETRLGGTAADWRFAQ
                     HVGGLAFPIQLFGAGHVAQALVRQLQLLGAQITWVDTRDGAFDGLDVTDVRTVLTDVP
                     ESEVARAPAGSFFVVMTHSHALDFSLCETIFRRRDFAYFGLIGSRSKRRSFEHRLVDR
                     GLNPERLDELVCPIGIAGIVDKAPAAIALAVAAEIFQVHEARRLVGDAARPRLIDNPV
                     QPE"
     gene            complement(77218..79566)
                     /gene="xdhB"
                     /locus_tag="PLH85_00850"
     CDS             complement(77218..79566)
                     /gene="xdhB"
                     /locus_tag="PLH85_00850"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HNB63136.1"
                     /translation="MSPSGATPVGRSVPHESAPLHVSGKAQYIDDLPEMAGTLHAAPG
                     LSTCARGRIRAMDLSAVRAYPGVRCVLTAADIPGENNCGPILHDDPIITGDLVEFYGQ
                     VIFVVAAETREAARQAARLARVEYAAEPPILDMDSAIAAESWVLPPFVMTRGAVEQAF
                     ENAPHRLSGAARLGGQEHFYLEGQISCAVPRESDTIHVFCSTQHPTEMQQMVAHALGW
                     RSHQVSVECRRMGGGFGGKESQSAQWACLAALLAVKTGHPVKLRLDRDDDMVATGKRH
                     PYQYRWESAFDDAGRILGLRLEMASDCGYSADLSGPVNDRTICHIDNAYYLDAVALRS
                     LRCKTNRVSNTAFRGFGGPQGMFVIETVLDDIARHLGLDALAVREANFYDVVPGARST
                     TPYGMTVEDNVAPALVAQLADASDYGARRAAILAFNASSPVIKRGLALTPVKFGISFN
                     ATHYNQAGALVHVYTDGTVLVSHGGTEMGQGLYTKVRQIVAHEFGLPLEDVRLSSTDT
                     SRVANTSATAASSGTDLNGKAAQAACQHIKARLAAFVVELVQGAVAAEQVRFVDARVQ
                     AGEASAPLFDDSFAALVMRAYRARIQLWDAGFYRTPKIHFDPQTKLGRPFYYFAYGAA
                     ASEVAIDTLTGECRVLRVDVLHDAGRSINPALDIGQIEGGFIQGMGWLTSEELWWRPD
                     GPQAGRLMSHAPSTYKIPTATDLPDVFNVRLFDNANVEDSIHRSKAVGEPPFMLALSV
                     FMALRDAVAAALPAGQSPRLEAPATPEAVLRALGCLAPEDGA"
     gene            complement(79575..81071)
                     /gene="xdhA"
                     /locus_tag="PLH85_00855"
     CDS             complement(79575..81071)
                     /gene="xdhA"
                     /locus_tag="PLH85_00855"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HNB63137.1"
                     /translation="MPIRPIQFYFRGAVQSVQGPAPTRTVLQFLREDLHQTGTKEGCA
                     EGDCGACTVVIGELAKGPEGDELRLRAVNACIQFLPALDGKALFTVEDLSADGHLHPV
                     QQALVERHGSQCGFCTPGFVMSLYALYENTPICPPRAEIDHCLSGNLCRCTGYRPIVD
                     AARDAFLAPRRSLERAPIVAALKSLAELPPLDYAAAGRHSYGPRTLTELAELRERLPD
                     ARLVAGGTDVGLWVTKQGRDLGDVIQLGGVAELKRIRVGAGQLEIGAGVSLTDAFAAL
                     TAFEPHWDELARRFASTPVKNAGTLGGNVANGSPIGDSMPALIALGCRVVLQQGAHTR
                     EMPLEAFYLDYQKTALQRGEFIRALVLDSPLPPLNRQFRAYKVAKRFDQDISAVACGF
                     AVHLDDEHIVRGARLAFGGVAGTPKRADAGEAALLGRPWTPDTVRLAMAALREDFAPL
                     TDVRASAAYRLSVAANLLQRFWLETTAPPDGLPARLSALGPIQPEERS"
     gene            complement(81188..81682)
                     /locus_tag="PLH85_00860"
     CDS             complement(81188..81682)
                     /locus_tag="PLH85_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNB63138.1"
                     /translation="MSDILTAADETFLRHCIELSRRVRDGGKHPFAAIVVDELGRIVA
                     EAGNDSVPPDGDPTRHAELVAAGLAARRLTPGQLSRATLYTSAEPCAMCAGAIYWCGI
                     GRVVYALSEHRLLGLTGDHPENPTFSLPCREVFARGQRRIAVTGPCLEDEAAAPHAGF
                     WTRE"
     gene            81843..82922
                     /locus_tag="PLH85_00865"
     CDS             81843..82922
                     /locus_tag="PLH85_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB63139.1"
                     /translation="MLLRRLKSFTAVAVMALAGLHAAHAAEPIKAGFVYIGPTGDHGW
                     TYSHDEGRKLLEAQSNGSVKTTFVENVPETADAERVFRDLAQKGNKVVFGTSFGYMNQ
                     MAKVAKAFPNTIFMHATGYKTAPNLGVYDVRTYEGAYMLGVVAGKMSKGNKLGVVASI
                     PIPEVIRNINAFTLGARSVNPAITTRAIWVNSWFDPGKEREAALALIAQGCDVLMQNT
                     DSPAVVQAAQEKGVLAFGWDSDMTKFGGKAHLAASVLNWGVIYKKTVDEVAAGTWKSG
                     DIWWGVKEGAVNIDNFGPAVPAPVKKLAEERRDAIKAGKLHPFTGPLKEQSGKEFLAA
                     GKIFGDGDLKKMNFYVEGVEGSIPK"
     gene            83032..84069
                     /locus_tag="PLH85_00870"
     CDS             83032..84069
                     /locus_tag="PLH85_00870"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNB63140.1"
                     /translation="MPPPLAADAALDFACRMPKAELHVHIEGTLEPELAFALARRNGI
                     ALPYADEDALRKAYAFDSLQSFLDLYYACADVLRTPDDFRDLMLAYLERAVADNIVYT
                     EIFFDPQTHTARGIPFETVLRGLEDGLYHARRLWGIEGRLILCFLRHLSEEDAFATLA
                     EAEPFLERIHGFGLDSSERGHPPAKFARVFARCRALGKPIVAHAGEEGPPAYISEALD
                     LLGARRIDHGVRAVEDAAVVDRLIRDAIPLTVCPLSNLRLCVFPSMQDHTLGRLVAAG
                     ACVTLNSDDPAYFGGYLNDNIRAVHAAFAFDIPTWYQLARNSFQASFASPTEKQQWID
                     RLDAARAATGL"
     gene            complement(84344..84418)
                     /locus_tag="PLH85_00875"
     tRNA            complement(84344..84418)
                     /locus_tag="PLH85_00875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84383..84385),aa:Arg,seq:cct)
     gene            complement(84502..84690)
                     /locus_tag="PLH85_00880"
     CDS             complement(84502..84690)
                     /locus_tag="PLH85_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HNB63141.1"
                     /translation="MNIAPTPSTSAANGADPVSIAMLRKTLDQQQANAQQLLQALPQP
                     AKAPDPTATVGRNVDVFA"
     gene            complement(84695..85180)
                     /locus_tag="PLH85_00885"
     CDS             complement(84695..85180)
                     /locus_tag="PLH85_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNB63142.1"
                     /translation="MPSFDITSEVDMVALRNAVDGANRKITGRYDFKGSDARVEQTEK
                     LLTLHGDSDFQLEQMLAILLPEMTAKKIDVRCLDHGDVQKVGGNKVKQELKVRVGIEQ
                     EQSKKIVKLLKDSKLKVQASIQGDAVRVSGAKRDVLQECIALVRKDITDYPLQYGNFR
                     D"
     gene            complement(85194..85517)
                     /locus_tag="PLH85_00890"
     CDS             complement(85194..85517)
                     /locus_tag="PLH85_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNB63143.1"
                     /translation="MTQNVQFDTVSVVKKANVYFDGKCVSHSIVLADGTKKSVGVILP
                     ATLTFNTGAPEIMEGVAGACRVRLKGETEWKTYGAGESFNVPANSAFDIEVSGEPYHY
                     VCHFG"
     gene            complement(85537..85770)
                     /locus_tag="PLH85_00895"
     CDS             complement(85537..85770)
                     /locus_tag="PLH85_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNB63144.1"
                     /translation="MDPSAPLFFGLTVAEFEDISLKLCVGGLIAYMVFIIGQLAWESK
                     AGKYGAIWMFVGLGVGFIGFIAKGLIQRLLGIE"
     gene            complement(85782..87011)
                     /locus_tag="PLH85_00900"
     CDS             complement(85782..87011)
                     /locus_tag="PLH85_00900"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNB63145.1"
                     /translation="MSDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LEPARAKALQFGIKPENIFIDDLREEFVRDFVFPMFRCNTIYEGEYLLGTSIARPLIA
                     KRQIEIARATGADAVSHGATGKGNDQVRFELGYYALMPNVKVIAPWREWDLLSREKLL
                     AYAEKNGIPIEMKHKQGGSPYSMDANLLHISFEGRHLEDPAAEAEESMWRWTVSPEAA
                     PDAAEYLDLEFEKGDLVALNGTRMKPHELLARLNELGGKHGIGRLDLVENRYVGMKSR
                     GCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASMIYTGYWWSPERLALQAL
                     IDQTQQTVNGWVRLKLYKGNVIVVARDSKTDSLFDPTIATFEDDQGAYNQKDAHGFIR
                     LNALRMRIAANARAKRG"
     gene            complement(87075..87998)
                     /gene="argF"
                     /locus_tag="PLH85_00905"
     CDS             complement(87075..87998)
                     /gene="argF"
                     /locus_tag="PLH85_00905"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNB63146.1"
                     /translation="MKAPRHYLQFRDFSRAEYDYLFARTRWIKDKFKRYEPYHPLFDR
                     TLVMIFEKASTRTRLSFEAGMQQLGGSAIYLNTRDSQLGRGEPVEDAAQVISRMSDVV
                     MIRTFEQSIIERFAEYSRVPVINGLTNEYHPCQILADIFTFIEHCGPIQGKTVAWVGD
                     SNNVCNTWLQAAEVLDFHVHVSTPPGYEVEPERAGLIGTGHFAHFDDPMEACRGADLV
                     TTDVWTSMGFEAENEERMRDFAEWRVDEDMMRVANPGAVFMHCLPAHRGEEVTAEVID
                     GPQSVVWDEAENRLHVQKALMEYLLIGKVES"
     gene            complement(88017..89192)
                     /locus_tag="PLH85_00910"
     CDS             complement(88017..89192)
                     /locus_tag="PLH85_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNB63147.1"
                     /translation="MPHLMNTYGRLPVAFSHGQGSWLFDTEGKRYLDALSGIAVSTLG
                     HNHPRLVRAIAEQAAQVIHTSNLYAIPRQEQLSDRLAALAGMDEVFFCNSGCEANEAA
                     IKLARLYGHGKGIAEPAIVVMEHSFHGRTLATLSATGNAKVQKGFEPLVGGFVRVPFD
                     DLGALEQATAGRSDIVAILLEPVQGEGGIHVAHSAYMEALRGMCDANGWLLMFDEVQC
                     GVGRTGKWFGHQNAGVKPDVMSLAKGLGSGVPIGACLASGRAAGVFKPGNHGSTFGGN
                     PLACAAALETLAVLDDERLLDNAGRMGQALREGFAVALAGLPGVIDIRGAGLMIGIEL
                     DRPCAELVGRALQAGLLINVTADKVIRLLPPLNFSAEEAAALVEGLAPLVKEFLAGQ"
     gene            89480..89773
                     /locus_tag="PLH85_00915"
     CDS             89480..89773
                     /locus_tag="PLH85_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HNB63148.1"
                     /translation="MIQKLEHFMIVGRTSDGKTFRPSDWADRLCGIMSAFGADNRMTY
                     SPYVRPACNLRGDKTVLVDARIHDIEPLAYNFLKNFAKDNDLQVEVLPDDHFD"
     gene            complement(89870..>91915)
                     /gene="lon"
                     /locus_tag="PLH85_00920"
     CDS             complement(89870..>91915)
                     /gene="lon"
                     /locus_tag="PLH85_00920"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNB63149.1"
                     /translation="PNNEVEAMRRAIISQFDQYVKLNKKIPPEILSSLSGIEEPGRLA
                     DTIAAHLPLKLEQKQEILEMFNPGERLERLLSQLETELDILQVEKRIRGRVKRQMEKS
                     QREYYLNEQVKAIQKELGEGEDGADLEEMDKKIKASGMSKEAAAKAQGELKKLRLMSP
                     MSAEATVVRNYIETLVGLPWKKKSRISKDLAEAEKVLDQDHYGLEKVKERILEYLAVQ
                     QRVDKLKAPILCLVGPPGVGKTSLGQSVARATNRKFVRMSLGGVRDEAEIRGHRRTYI
                     GSMPGKILQNMTKVGVRNPLFLLDEVDKMGQDFRGDPSSALLEVLDPEQNATFVDHYI
                     EVDYDLSDVMFVATANTLNIPAPLLDRMEVIRLSGYTEDEKVNIAQRYLVPKQMKNNG
                     IKREELSVTEDAIREIVRFYTREAGVRGLEREISKVCRKVVKAIVLGKRKAKVVVNAK
                     NIDKYLGVRKYTFGVAEKQNQIGQVTGLAWTEVGGELLTVEAVALPGKGKIMTTGKLG
                     EVMQESIQAALSVVRRRSKSLGIAADFYQNSDIHIHLPEGAIPKDGPSAGIAICTGLV
                     SVLTGIPVRCDVAMTGEITLRGEVLPIGGLKEKLLAAVRGGITRALIPSENVKDLTEI
                     PDNVKNALEIIPVKWIDEVLELALERRPEPLPDTPAPEVPVAAAADQPADNGGVVKH"
BASE COUNT        15400 a        30145 c        30837 g        15433 t
ORIGIN      
        1 ccgcatagtc cttccccgcg gcgtcggagt ccttccacga tagccaaaac ccttaacacg
       61 cgggcccaac attcgaatcg cggctatcaa tgagcttccc cgcatccgcg gattccttcc
      121 ccaggcccgc ggggtccttc tctgcactcg cggattcctt ctctgcactc gcggattcct
      181 tctctgcgac gtccaccaag caccccgcgg cctcaaagtc cttccccgca cgcaggggga
      241 agcactccgt tgcccgaaag tgtttccccg ccccgtcggt gaagcactcc accgcgtgaa
      301 cgaagccctc agccccgcgc gcttgcgctc agtccccgtc cagctccggc agcgccgggg
      361 gcaggtcgtc caggcgggcg gcttcgatct tctggaagtg ggcggtgatc ttgcgggagg
      421 aggtctgcac gtcggccacg tcttcgccgg cctggcggat gtgggtggcg agtttctgca
      481 tgcgttcgtc gaagcgggcg aagtctttgg cgagcttgcc gagggcgtcc tggatgacgt
      541 ggatctggcg gcgggtctcc acgtccttga gcacggcgcg ggcggtgttg aggacggcca
      601 tgagggtggt gggcgagacg atccacaccc gtttggcgag ggcgtgggtg acgacttccg
      661 ggtggtaggc gtggatttcg gcgaagacgg cttcggcggg gacgaacatg acggcgccgt
      721 cggaggtgac gccgggcagg atgtacttgc tggcgatggc gtccacgtgc ttcttcacgt
      781 cggcgcggaa ggcggcctgg gcggtgcggc ggtcggcgtc gccctggtcg gcggcgaaca
      841 tgcggtggta gttctccagg gggaacttgg aatccaccac caccaggccg gtgggcgcgg
      901 gcaggcgcag cacgcagtcg gcgcgggtgc tgttgggcag ggtgtattgg aagtcgaagg
      961 cgtccggcgg cagggcgttg cgcaccaggg cttcgagctg gacttcgcca aaggcgccgc
     1021 gggcgcgctt gtcgccgagg agttcctgca ggctgacgac gttggtggtg aggtcgccga
     1081 tctttttctg ggcttcgtcg atggtggcga ggcgggccat gacgttgacg aaggtctcgt
     1141 tggttttgcg gaagccttcg tcgaggcgct ggttgacgtg gccgccgatg gcgtcgaggc
     1201 gttcgcccac ggtgcggccg aggccttcga tgccgtgggt gagctgggcg gaggcggcct
     1261 gcaggccgga ctggaggagc tcccggtcgg cgcgggcgtg ttcggagagg gtgcgcaggg
     1321 tctcggcgag gagttcgctg cgcaggcgtt cctgcccctg ggcgacgccg gcgcccagct
     1381 cggcgaggcg cagggcggtc tcttcgcgct gggtggcgag gcgggcctgg aggcgctcgc
     1441 cctgctccgc cgtaaggtcg gtgacgcggt cggtggcggc ggaggtggcg gcctggagtt
     1501 ccttgacgaa ctggagctgc tggtggccca gcaggtgttc gacccggtcg gcgaggctgt
     1561 cggcaaggtc ctgggcgacg gcgtcctgac gacgggccag ggcggcgacc tggaaggcca
     1621 gccagacgag gaggacgagg ctcaggccga gggcggcgag gagccagggg aggatttggg
     1681 tggaggcggt gtcgggcatc atgggcgcgg agtatacccg cgccctgccc ggcttccgtc
     1741 ggtggcgggg acggtgtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     1861 cgcggagtat acccgcgccc tgcccggctt ccgtcggtgg cggggacggt gtgtggcggc
     1921 cggccgcggg gatcagccga gggcgcgcag gctggccagg ggcggcgtaa ggagcaggcg
     1981 gcgggtgccg aaccagccgg cgagggtcac ggcgaggccg ccggccagcg cgccgacgag
     2041 caccggcagc aggctggggg tgtagggcag cgtgaacacg aagcgcccga ggctccagcc
     2101 gatgccgctg gcggcgaccc cggcgaggct gccggagagc acgcccagca cggcaaattc
     2161 ggccagcagg gcggcgcgca gctgggcgtt gcgggcgccc agggtgcgca ggatggcgag
     2221 ctcccgctcg cgctcgtcgt gggtggattg cagcgcaccg aggagcacca ccacgccggc
     2281 caccacggca aagccgaaga cgaactgcac cgcggcgacg agctgatcca ccatgccctg
     2341 cacctgggcc agcatggcgg ccacgtcgat gaccgtgaag ttggggaacc gggcgaccag
     2401 gcgcggcacg aagtcggtct gcgccggcgg caggtagaag ctggtgatgt agctcgccgg
     2461 aaaatccgcc agcaggccgg ggctggcgat gacgaagaag ttcacccgca tggagtccca
     2521 gtccagcttg cgcaggctgg cgatgggggc ttcgacgatc tcgccggcca cgttgaagcg
     2581 caggacgtcg cccatcctga ggcccagagt ctgggccagg ccctgctcca ccgagaagcg
     2641 tggcgtggtg tcgtggccgt gccaggtgcc ggcgatgacc cgattgcccg gcggcagcgc
     2701 ggcgtcgtgg gagaggttga agtcccggtc caccaggcgg cgggcgcggt cgtcggtgta
     2761 gtcgtcgggg cccaccgggc ggccattcac ggcgaccagg cggccgcgga tcatgggcag
     2821 cagcacgggt gcgggccggc cggcggcgcg gaaggcgtcg cgcagggggt cgagctggtc
     2881 cggctggata ccgatgatga agcggttggg ggcgtccggc ggggtggcct gctgccagct
     2941 ggcgagcagg tcgccgcgca ccagggtgag gagcagcagc gcggtgaggc ccatgcccag
     3001 ggccagcacc tgcaccaggc tggcgccggg tcgccggcgc agggcggcga ggccgtagcg
     3061 ccagccgctg cccgcaccgg cgcggatgcg tccgacgccg cgcagcacga tccagccgac
     3121 gccggcgaag acaccgaagg cggcgacgaa gccgccggtg acccaggcgc cgagcgtgac
     3181 gtcccgcgcc acgagcatca gcagcccggt gagcacggcg gcgccgaggc tccaggcgag
     3241 gccgtcgcgc accgccacgc cgcccagctc gcggcgcagc acgcgcaggg tgggcacgcg
     3301 gccgaggcgg atcaggggcg gcagggcgaa gcccagcagc agcacgaggg acacggcgag
     3361 cccgtgggcc aggggccaga cgctaggcgg cggcagctcg aagctcacca ggctgccgag
     3421 gaagcgttcc agggcgtatt gcacgccaaa gccgagggcg ctgccggcga ggccgccagc
     3481 caggcccagc agggtgaatt cgccgatgaa gaggcccagc agggttgtct ggcgggcgcc
     3541 caggcagcgc atcacggcgc agccgtccag gtggcgttcg acgaagcggc gggtggcgag
     3601 gcctaccgcc accgcggcga ggaccaccgc gagcatggcg gcgaagcgca ggaagcgttc
     3661 ggtgcggtcg agggtggcgc ggatctcggg gcgggcgttg tccacgcttt cgagggattc
     3721 gccgcgcccc aggcgggcct tggcccaggc ttcgtagccc gccacggcgg cgatgctgcc
     3781 ggccagatgg aggcggtagg tgacgcggct gccgggcacg atgaggccgg tggcaggcag
     3841 gtcggcggcg ttcatgacga tgcgcggcag cagggcaaag aagttggcgc cccggtcggc
     3901 ctcgaagctg agcacggcgg ccatgcgcag gcgcgagcgg ccgaggccga cgagatcgcc
     3961 agggtgggcg ccgagggccg tcgcgctgcg ttcgtcgagc cacacctcgc cgggcgcggg
     4021 gatgccgcgg gcgggggcgt ccggctggtt gaggccgggg gcgatgcgga tctcgccgcg
     4081 cagggggtgg cccgcctcca ccgacttgac gccgacgagc tgggtcgcgt cgccgaggct
     4141 ggccatgctg gtgaaggtgg tggattcgac gctggcgagg ccgcggcggg cggcttcggc
     4201 gcggaagtcc ggcgccagcg ggtggtcggc ggcgatgagc aggtcgccgc ccaggagctg
     4261 gctggcctgg cggttgaggc cgcggccgac ccggtcggtg aagaagccga cgctggtgag
     4321 gctggcgacg gcgatgacga gggcgacgag gagcagaccc agttcgccgg cacgcaggtc
     4381 gcggcggagc atgcggaagg acagggcgag gcgctggcgg acggtcatgg agcgtgaggc
     4441 cgcggcggcg cgggaagatt cccggctgcg gcggaaattt tgcggtgcgt cacgctagca
     4501 caggatgatt gccctttcag ccgccctgcc ctgcctgccg gaaacgcccg gccggggcgg
     4561 accgttgcgc ccggtgtgtg ttccggctgc cgaggggcac cggccggggt acacagacaa
     4621 acggcacccg aaggtgccgt tgcccgcgtc tgcagaaggc ctcagttctt ccgccgacgc
     4681 attcccagga gcgcgatgag tgaagtgccg cccaggagcg ccaggctcga gggttccggc
     4741 accttggtgt aggtgccggt gacggctgcg atcttgatgt aatcgttgcc gctgcccagg
     4801 gtggcgccgt tgggcagggc ctggaccagg gtgttgaggg cgccgatcag ccagtagcgg
     4861 gcgctgacgt tgagatcatt gaccgccagg gggatgtaac ccgccgagtt gctggactgg
     4921 gacggcgcgc cgccggccgc atccgcgtag ttggcggtgc cggtggtgcc ggcgctggat
     4981 ttgacggtgg tccacccgct gctcagcagg gaggcgtagc cctggttggc ggaggtgaat
     5041 gtgggggtgc cggtgccggt gtaggccagc acggtcaggt cggagtcgga gtaccaccag
     5101 ccgacggtga cgctctggag cttcaccgcg ttgcccgcgc cgaagtcgaa gagcatcgag
     5161 tcgtagcggg cgttgttgtc catcgtatgg ccaggcgacg tggtgttggc gatggtgcct
     5221 tcgttggtgt cgccggcacc ggtggcgccg tcccggttgg tcacgcccag accgccggag
     5281 taggtgccca ggtaagcgct ctcgatgtag gcgttgcccg cagtggtgct gttcttggtg
     5341 tcggaccagg ccgagatggt caccgtggag ccggtgctgc cgtaggtccg ggtgttgccg
     5401 aagttaccgg cgccggtttc ggtccccccg ctggtgaggg tccaggcggt cgcggcgtgg
     5461 gcgccggcga cgctgccgat cagcagggcg aaggtcaatg ccgtttggct gggaatgcgt
     5521 ttcatgtcgg gaggtcctat tttcgtttcg acacggctgt cgttgttgtc agatatgtag
     5581 caattattct gccaattcgc tatttacttg tttttgttta aaaatcgcag tgcaccacgt
     5641 ttatggcaag gcaaactgta aaatctatcg acaacgttaa aaattgttta aacacccagc
     5701 tcgcggcgca gattttgtat ttcaatgtcg tagccgcttc cattcgtact gttcagcgcg
     5761 ccttgcagtt cagcgcgtgc ttcgtcccgc aggcccttgc gaaggaggac ccaggccagg
     5821 tggtagcgga tctcgcggga ctcgggggcg cgcaggcggg cttcgcgcag ggctttcaga
     5881 ccgatgtcca gcgccccgct acgggcccgc gcccagccga cggtgtcgag cacgttggcg
     5941 tcgccgggcg caaggcggta ggcggcttcg gcggcgttga gggcgcccgg gtcgttgagg
     6001 gccatcagga tctgcgccag gttgttggcg gcgttggcgt tcgccggctc ggctgccagc
     6061 acgccttcgt agaggcggcg tgcgtcggcc agatggccca gacggaaatg ggattcggcc
     6121 agggcgctgc gggcggtcag gtcgttcgga tgggtgcgca gccaggtgtc cagcagggat
     6181 acggcgcgat cgccctcccc ggcctggaac gaggcctcga caccccgcag gacgagcgcc
     6241 agggacgggg ccttgtcgaa ggcgctgcgg tagagacggg ccgcatcgcg gggctgttgg
     6301 cgggcgcggg cgaggtcgcc ggccaggcgc agggcctcgg cgctgttgcc gctgcgggtc
     6361 gcgaaggatt tgaggagggc gtcggcgcgg ttgagatcgc cgagggccat ctcgctttcg
     6421 atctgcagga tctgggctgg cacgaggccg gcgttgcgcg ccagggcctt ctcggcgctg
     6481 gccgacgctt cgcggaaggc gccggcatcc agttgcaggc gtccgacggc caccagctgt
     6541 ccgggttcaa gaccgggcag gcgggacagg gtattgaagg tgctgcgcgc atcggccaga
     6601 ttattggcgg ccaggtgcgc gcggccgagg gtttcgagga cgagcggatc gtccggacgg
     6661 ctgagcgcca gctgcttggc caccgcgacg gccggcgcag gcttgccgag cttctggtag
     6721 agggccagaa gctcctgggc gacggtcggg tcggcgggct tgagggcata ggccttttcg
     6781 agcagggcca acgcgtcggc gctacggccg gcgccaagct ccaggcgggc gttttcggtg
     6841 agcacctgca catcggccgg gtgtgccttg agcagcgcgg cgaggcgctg gcgggccgcg
     6901 tcggcgcgtc cttccacgag atccaggcgg gccaggttca actgggccgg aaggaagccg
     6961 ggcgccagcg tgatggcctg ggtgtattcg gcgcgggcct cggccggctt gcgggccgcg
     7021 cccttcacgg cgcccagcag gttgtgggcg gcgggatcgg acggcaggcg ccgggtggtg
     7081 gcttccgcca ccgccagggc gttgcggagg tctccgcggc gcaggtacag ggtggacagg
     7141 gcggtcgcca cgttgagttg gcccggctcc ttgtcgaacg cgctgcgcag ggcgctgagg
     7201 ccgatgtcgg gctgcatgtt ggcgatgcgg gcgaagccca tctgggcggt gatgcccgga
     7261 ttgccggtga ggcgcgcggc ggcttcgagg gcgtcggcgg cgtcccggta gcggcgctgc
     7321 tgcatgcgca gggcggccag ggtggtaagg gcctggggat cggcgtcgcc ggaactgatg
     7381 aggggctgga gcaggtcgcc gacgcgggcg gtctcgccgg cggcgaggta gatgttggcg
     7441 agcatcttgc ggccggcggc gtcgccgggc atgcgccgga cgtagagttc gaggagcgca
     7501 cgggcgcgct caccgcggcc cagggcctgg ctggcaaggc cgccgatcat cagcagggtg
     7561 ggctggcggc cgacgaattc ctggggcagc tcgtccacca ggcggaccac gtcctccagc
     7621 tgggcgcggg aggcctgggt gtcgccgcgc tgggcataga ccaggctctt gaggtaggcg
     7681 acgcggggct cgcggggtac cagggcggcg gcgcgctcca gatccggcag ggcctcgttg
     7741 gcgcgcttca ggtcgatgag cagggacgcg cgtccgacga gagcgtcgag catgcggggg
     7801 tcggcggcga tggcgcgatt gaaggcctcc atggctccgc gggcgtcgcc ggcgccctgc
     7861 agcaggccgg cctgcaggtg cagcaggcgg gcgtcgccgg gggcgatgcc cagggcggtg
     7921 tcgaggacct ggcgggcgcg ggcgctgtcg cggctgcgca gggccaggtc gatttcggcg
     7981 agcaagggcg acagggcgcg gggatcggcc tcacgcgcca gcttgacctc ggcgacagcg
     8041 gcacgcatgt cgccccggtc ggcctgggcg gtggcgcgca tggcgagcac ttcggcgcgc
     8101 aggcgcaggg gcaggccgtc ggccttgatg cgctccagca gcttttcgct gttgccgaac
     8161 atcatcaggg cctggccgta ttcgagggcg atctcggacg gattcacgcc caggcgcagg
     8221 gccgcttcga actcggcctc ggcggcggcg gcctggccgt ccttgagcag ggcgcgggcc
     8281 agcagcaggt gggcaggcag ctgttcgggg tcgcgctgga gggcgttctt ggcctggatg
     8341 atggcaccgg cggcgtcatt gcgggccaga cgggcggcgg catcctcgta gtagcgggtg
     8401 gcgctgtcgg aggcgcccgc ggcccagccg ggcagcgtca ggcacaggca caggagcgcg
     8461 gcgggcaggc ggcggagggt gacatgaggc tgttgggaag tcggcatttt cagacggcat
     8521 cgtgcgatga caggcgatac cgtctttgta atcgccggcc gggacacggg ccgtgacaaa
     8581 tgccgccaca gagcctgtgg cgccctgcaa ttccgtcacg ctgtagagtc gatcaccaga
     8641 aagtgaagac aaatggcacg gtttctccgc ggggagacgt gtgcggaggg gatgtgccgg
     8701 aggtccgccg ggcccgaaga atgcgccgga aaccgtaccg gtcggcgccc cgtcgggcgg
     8761 ggcgtggtgt tcaggcgtcg gcgagccagc gttgcacgag acgcacccaa taggcgacgc
     8821 cataggggat ggcggcgtcg ttgaagtcat agtggggatt gtgcagggtg cagccgccct
     8881 cccccggccc attgccgagc cagatgtagc agccgggctt ttcctgaagg aaataggaga
     8941 agtcctcggc gcccatgctc ggccggggat cgacgctgac ccgggcatcg ccgcacacct
     9001 cggcggccac gcgggcacag aattcggcct cggccacgct gttgatggtc ggcggatagc
     9061 cgcgtcggta ctcgaaatgg acgttcgccc cgaaggccgc accgacgccg gcgcagatgc
     9121 gttgcatggc gtcctcgacg cgggcctgca gttcgggtct gaatgcgcgg acggtgccgc
     9181 cgatgcgggc cacatcgggg atcacgttgt aggcgtcgcc ggcctggaac tgggtgacgg
     9241 acagcaccac cgcgtcctgg ggatccgtgc cgcggctgac caccgactgg agggcctgga
     9301 ccagagcggc tccggcgagc accgggtcga tgccctgatg gggcatggcg gcgtgggcgc
     9361 cgtggccgcg gatctcgatg tcgaagcggt cggcacaggc catcactgca ccgcgatgca
     9421 cggcgatttc gcccagaggc aggcccggcc agttgtgcag gccgaacacg gcgtccatcg
     9481 ggaagcgctc gaagaggccg tcggcgatca tctcgggcgc cccgccctcc ccttcctcgg
     9541 ccggctggaa caggaaatac acggtaccgt cgaattccgg gtgacggctc aggtgggccg
     9601 cggcgcccag gagcatggcg gtgtggccgt cgtgtccgca ggcgtgcatg cggcccgggt
     9661 ggcgggagtg gtgggcgaag tcgttctttt cctgaatggg cagggcgtcc atgtcggccc
     9721 gcaggccaat ggcgcggcgc ccggagccgg ccctgagcac gcccacgacg ccggtcttgc
     9781 cgatgccggt gtgggtttcg atgccgaggg cgcgcaggcg ttccgccacc aggccggccg
     9841 tgcggtgctc ctcgaaggca agctcggggt gcgcgtggat gtctcggcgg agcgcggtga
     9901 tgtcggcgtg atccgcccgc agggaatcaa ggaggtgcat ggtgcattcg cgtgtccggc
     9961 ccgcgggccg tatggttcaa tgatagcagc cgcctgccgc cctgctcccg atgaagctct
    10021 tctacgccga ccatttcgtc ctgcccctgc cgccggggca ccgcttcccc atggagaagt
    10081 acgcccgcct gcgcgagcgc ctgcaggctt cgggcctgtt ccgtccggac gatttccacg
    10141 aaccgccacc tgccaccgac acggagatcc tgcgtgccca tgacgcgggc tacctgcagc
    10201 gggtggtccg tgggcagctg acgccggacg agctgcgcct gatcggcttt ccgtggtcgc
    10261 cggccatggt ggagcgttcg cgccgatcgt ccggggccac gctggcggcc tgccgggcgg
    10321 cgctggagga cggctgcgcg gtcaatctgg cgggcggcac ccaccacgcc ttccgcgacc
    10381 ggggggaagg cttctgcgtc ttcaacgacg cggccatcgc ggcgctggcc atgcgcgccg
    10441 agcaccgtgt ggagcggctc gccatcgtgg attgcgatgt gcaccagggc aacgggaccg
    10501 ccgccatcct ggcgcatcgg gccgactgct tcacctgcag cctccatggc gcgaaaaact
    10561 atcctttcga caaggaacgc agcgacctgg acgtggaact gcccgacggt accgccgacg
    10621 gtccttatct ggacgccctg gcgatggcgc tggagaccgt gttcgcgcgc gccgatcccc
    10681 agctcgtgat ctacctggcc ggcgccgacc cctacgccga tgaccgcctt ggccgccttg
    10741 ccctgagcct ggacgggctg gccgcgcggg accgtctggt gctgggcgaa tgccggcgcc
    10801 gcggcgtgtc ggtggcgatc gccatggccg gcggctatgc tcgacagatc gacgacaccg
    10861 tggcgatcca cgcccgcacc gtcaccctcg cccggcagcg cttcgcgccg ggccccacgg
    10921 aggccccatg agccaagaat cccacatcgc cctgctcatc gacgcggaca actgccctgc
    10981 cgccaagatc gaggagatcc tcgacgaact ggccaaatac ggcgtgatca acatccgccg
    11041 cgcctacggc aactggaaga gcccggggct gaagagctgg gaggcggtgc tccacgaata
    11101 cgccatccag ccgatgcagc agttcgccta caccaagggc aagaacgcca cggattcggc
    11161 gatgatcatc gacgcgatgg atctgctcta cagccagaac ctggacggct tctgcctggc
    11221 ctcgtcggac tccgacttca cgccgctggt gatgcgcatc cgtgccaacg gcctgaaggt
    11281 cttcggtttc ggcgagaaga agacgccctc gcctttcgtg aatgcgtgct ccaagttcct
    11341 gtacctggaa aacctcgggg cgaccgacgc cgatacgccg gcatccgagg tgccgccagc
    11401 agcggttccg tccgcccggg ccgaacccga tggcggagcg ggacgcggtg ctgccgccgg
    11461 tgacgcgggc aaggccgccg agtccagcac gcgcccggtc gcccgcccca ctccgcggga
    11521 gctgcgcggc gacgcacgtc tggtcaatct gctgcgcaag tcggtagagg ccgccgccga
    11581 cgacgagggc tgggcggcgc tcggggaagt cggccagcac atctccaagc aggcctcttt
    11641 cgattcgcgc aacgtgggct acgcccgcct gcgggatctg atgcaggaca tcggcctgtt
    11701 cgacgtcagc cagcgggaca aggccatcta cgtgcgcgac aagcgcctgg cgcgcaagcc
    11761 cgcgccctga accgcccgcg ccggccaccc cgcgcgccgc tggctataat cgcccttccc
    11821 ccaagaggac tgcgcccatg cgattcgaag gtaccgagac ttacgttgcc acccccgacc
    11881 tgatgctggc cgtcaacgcg gctgtcaccc tgcagcggcc tttgctcatc aagggcgaac
    11941 cgggcaccgg caagaccctg ctggccgaag aggtggccgc cgcgctgggc atgcccctgg
    12001 tgcagtggca catcaaatcc accaccaagg cccagcaggg cttgtacgaa tatgacgcag
    12061 tgtcacgcct gcgggattcc cagctcggtg acgaccgggt caaggacatc ggccagtaca
    12121 tcgtgcgcgg gcccctgtgg caggcctttg aatccgacgt cccctgcgtg gtgctgatcg
    12181 acgagatcga caaggccgac atcgaattcc ccaacgacct gctgcgggaa ctggaccgca
    12241 tggagttcta cgtctatgaa acccgccagt tggtcaaggc gcgtcatcgg cccatcgtgt
    12301 tcatcacgtc caacaacgag aaggagctgc cggacgcctt cctgcgccgc tgcttcttcc
    12361 attacatcaa gttcccggac cgggacacga tgcagaagat cgtggatgtg cacttcccgg
    12421 gcctcaagcc ggccctcatc caggaggccc tgcaggtctt cttcggcctg cgcgaggtgc
    12481 cgggcctcaa gaaaaagccg tccacgtccg agctgatcga ctggctgaag ctgctggtgg
    12541 cggaggacat tccgcccgaa gccctgcatt cgacggaccg caaggtcatc gtcccgcccc
    12601 tgcacggggc gctgctcaag aacgagcagg acatgcacct cttcgagcgc ctggtgttca
    12661 tggcccagcg caaccgctga gcgcggcgcg atgctgatcg atttcttcct gcatctgcgc
    12721 gaacgcaagc tgccggtctc caccaaggaa ttcctgaccc tcctggaagc cctgcaggcg
    12781 ggtctggcgc ggcacagcct ggacgacttc tacttcctgg cacgcagttg tctggtgaag
    12841 gacgaagccc atttcgaccg cttcgaccag gcgttcggcg aatacttcaa gggcgtgcag
    12901 gcgattcccg gcctggacgt ggatctgccc gacgagtggc tgaaggccac gatgaagcgc
    12961 cacctcagcc cggcggacaa ggccaagctg gaaaagctgg gctgggacac gctgatggcg
    13021 gaattcaaga agcgtctgca ggaacagcgg gagcgccacg cgggcggctc gaaatgggtc
    13081 ggtacggggg gcacttcgcc cttcggcaac agcggctatc acccggaagg catccgcgtg
    13141 gggggcgaat cggccggcaa tcgcaccgcg gtgaaggtgt gggaccggcg ggagtaccgc
    13201 aacctggacg attccgtcga actgggcacg cgcaacatca aggtcgcgct gcgccggctg
    13261 cgccgcttcg cccgcctcgg cgcgcccgac gagctggacc tggacggcac catcgccgcc
    13321 accgcgcgca atgcgggcta cctggacctg ctcatgcggc cggagcgcca caatgcggtg
    13381 aaggtgctga tcttcttcga cgtgggcggc tccatggacc cccacgtgaa ggtctgtgag
    13441 gaactgtttt ccgcctgccg ggcagagttc aagcacctgg agtacttcta cttccacaac
    13501 tgcgtgtacg accacgtgtg gcgcgacagc acccggcgct ataccgagcg cacgccactg
    13561 gcggacgtga tccacaaata tggcccggat tacaaactga tcttcgtcgg tgacgcgacc
    13621 atgagtccct acgagatcct gcagcccaac ggttccatcg aatacgccag cagcgaggcc
    13681 ggcgccgtct gcctgcgccg tctggcggac gcctatccgg ccctggcttg gctcaatccg
    13741 gagccggagc gcacctggga gtttcgccag tccatcggaa tcattcgcga actgctcggc
    13801 gagcggatgt tccccttgac cctcgacggc ctgacgcgcg ccatcgcgca cctgtcgcgc
    13861 aagcactgag cccggaaaag gcatcggccg cggacgatgg ccgcggccga catgcagacc
    13921 acccgcagct gcgacttacg gcgcccggta gttgtcctgg gtcatcaggt cgatgccgca
    13981 ggggaggttc tcgatccagt cgaggaagcg gttcaccatc gccttgccct cgaagcggca
    14041 gccgtgctgg ggcacgatca tgtccacgtc cagctggcgc accatgtgca cccagtagcg
    14101 gcagatcttg tttgaaatca tgtaacggcg atggaagccg gccatggtga tgacgtgatc
    14161 gtcgaagttg cgcactggct cggcagcctg ggcgtggtct acaagggagg tgcccatgtc
    14221 gccggaaaag aggatcttgg cgaccggatc gtagaactgg aaattcccct cggaatgcat
    14281 gaagtgggcg ggtagcgcct tgatgcggct gtcccccagc gcgatgttca tgccctcgtc
    14341 ggggatgccg gtgatacggc tggtgtagtc cttgccagtg gtgaaatggg gcacgaagcg
    14401 cgcccacagg cgggaaatca tcacccggca atgggttgtc accatccact tgttgagcga
    14461 cgcgatgatg tccggatcgg cgtgggtggc gaagatgtaa tccagcccgc gggacgggaa
    14521 gaagcgctgc atggccatca gcaggccgtt gtaggtcatg ttgccgccag gatcgaccag
    14581 tgcgccgtgt tcgccgtcga cgatcaggaa ctggttgcac tggaccgcgt gatcggcctc
    14641 ttcgtccacc aggtcgtaaa aggccaggca ggcgtgcttg ccatcattga agagttctac
    14701 cgccatgggt gcgctcgtgt gtcaaaaagt cattatttgc aattgcgtga cactgtaacg
    14761 ggttccctga aagcctgaaa gcccttctct agattgcgat gaggccgatg ccctgtattg
    14821 cagtgcaaga atggagtatg gttcgcactc ccgatttcaa ccgctggaag attgttcccg
    14881 atgcgccgct tcgtccgctc tttcgccgtc acctgcctcc tccccttgag cgtgtttggg
    14941 atgcccgctg ccgctcagag tctgtcctcc tcgtctgcag gaaaggccag aatgggcatg
    15001 tcgtccgggt tcggctcatc cgggatgggc tcggccaaca cactctccgg cggaatcgga
    15061 acccgcagcc tactggattc caaacagaac ggcccgcaga tcaaggattt gcggcgcccg
    15121 tccgcgtccg aatgtgcgat gcgtgaactg gcgggcaaga agtcgccggg catgacggcc
    15181 aatgggcaag cgggcagcgg caaagtgacg acgtacacgg atgcatcgtc cctgcgcgga
    15241 cgtagtggcg acatctacgg tgattgcgac ggagcccagg gaatgcgtcg caggggcgcg
    15301 ggaggaagca ccggaacgtc gtcgttcgtc gggatcggta gcgctacacg tccccagacc
    15361 gcctacgacc ggcgtaccgg taccttgaag gctgccaagg acggctatgt gaaccgtggg
    15421 gcgaccagtt ccatgatgcc caagcatccg gagtccgctt atgacccggt gaccggcacg
    15481 ctcaaaccgc cccgggtgca aagcgcgtac cagatctatc agcagcaata aaacacaggg
    15541 cgatgcagaa cggccctgga ggccctgcat cgccctgggt tgcggagcag cgcggccgga
    15601 gggcggttga acccttccgg cacgttcgac ctcagcccgc gttcatcgcc agcatcagct
    15661 ggttgatccg cttcacgaag ctggccggat catccagggt gccgccttcg gcgagcagcg
    15721 cctgatcgaa gagcaccgcc gcccagtcgt caaactggcc ggtctcgtac ttgagacgca
    15781 gcaccaccgg gtgggccgga ttgatctcga ggatgggcct ggacatgggc atgctctgac
    15841 cagccgcctt catcaggcgg gccagattca tccccatgtc gtgctcgtcg gcgaccaggc
    15901 aggccggcga atcggtcagg cggtgggtga tgcgcacgtc cttgaccttg tcaccgaggc
    15961 tgcccttcat cttgtcgagg agttccttga attctccggt ctccttctcg acggcggcct
    16021 tctcggcttc gtcctccagc gtgcccagat ccaggccacc cttggcgacc gaggccagcg
    16081 ccttgccgtc gaactcggtg aggtggccga cgacccattc gtccacgcgg ttggacagga
    16141 gcagcacctc gatgcccttc ttgcggaaga tttccaggtg cgggctgttc ttggcggcgt
    16201 tgaagctgtc ggccgtgacg taatagatct tgtcctgccc ttccttcatg cggccgatgt
    16261 aatccgccag ggagacggtc tcgtcagccg tgtcggcctg ggtggaggcg aagcgcagca
    16321 gaccggcgat cttctccttg ttggagaaat cctccccgac gccttccttc agcacctggc
    16381 cgaattcctt ccagaaggtg gtgtacttgt ccttctggtt ctcggccagg tcttccagca
    16441 gacccagcac cttcttcgtg cagccggcgc gcagggtgtc gatgtccttg gattcctgca
    16501 ggatctcccg cgacacgttg aggggcagat cgttggaatc caccaccccg cgcacgaagc
    16561 gcaggtagag gggcatcagc ttctcggcgt cgtccatgat gaagacgcgg cgcacataga
    16621 gcttgacgcc gtgacgggcc tggcggtccc acatgtcgaa gggggcatgg ccggggacgt
    16681 agagcagctg ggtgtactcg tggcgacctt ccacgcgaga gtgggtccat gccagcgggg
    16741 gctcgtagtc gtggcccacg tgcttgtaga actcggtgta ttcctcgtcg ctgacgtcgg
    16801 acttgggccg cgcccacagg gcgttggcct tgttgacggt ctcgtcttcg tcggtgacga
    16861 cctgttcctt cttgtcctcg tcccagcttt ccttcttcat cacaatgggc tgaagaatgt
    16921 ggtcggagta cttgcggatg atggactgga gcttccaggc ggacagcaga tcgtcctggt
    16981 cctcgcgcag gtggagggtg atctcggtgc cgcgggatgc cttttcgatg ggctcgacgg
    17041 tgtattggcc ggccgtgtca ccggtcatgg tgcactccca tttcaccgcg tcaccggccg
    17101 acagcccggc ccggcgggtg cgcaccgtca cggtgtcggc aacgatgaag gccgagtaga
    17161 agccgacccc gaactggccg atcaggtgcg cgtccttctg ctggtcgccg gtcagcttgc
    17221 cgaagaactc cttggtaccg gatttggcga tggtgcccag gtgggtgacc acttcgtcac
    17281 ggctcatgcc gatgccgttg tcggcgatgg tcagggtccg ggcggccttg tcgtacgcga
    17341 cgcggatctt cagttcggca tcctcggcca gcagggcgcc gttctcaagg gcctcgaagc
    17401 gcagcttgtc gcatgcgtcc gacgcgttgg agaccagctc gcgcaggaag atttcccggt
    17461 tggaatacag ggaatgaatc atgaggtgca gcagctgctt cacctcggcc tggaagttca
    17521 tcgtttgggc ggacatctgt tcgacagcca taaagcttgc tccataggga ccgtaaacgt
    17581 cccacatatg ggtgcgtcca cgcatctttc aagtcccgtc cgcatctttc atggaacctt
    17641 cccacgcttc accgtccggc ttcgccgaat ccgaccgccc ggcggatgtc ctgcctgccg
    17701 gaacgtcgaa gggccgacgt ttcagccgac agcggatcgc gatctggggc ggtttcgcgc
    17761 tgcttgccgc gctcgtggcg gccgtcgcgg tgtccctgct gcgctacgac cgggcccagg
    17821 ccctgcatcg cgccgaggcg gaactgcggg gcctgacccg cgtgctcgaa gaacacgtcg
    17881 cccgcacctt cggcgagacc gacagtgcac tcagcgacct tggccagcag ctgagtggcc
    17941 gtctggggag cgacgcgccg tcggagcggg acctcaacgc tctcctccgc cagcgcgcag
    18001 cccatctccc gcaggtcgaa tacctgtatg tggagcggga ggacggcagc ctcctcgcgg
    18061 acacgttcga cctgccgcgc ggcgcggaag ccgaagcgcc gaagcgcggt ctgcccaccc
    18121 tgcacgacgg cctcgagatc gcggcgcccg tccgcagtgc ccattcgggc cactgggtca
    18181 cccccgtcaa acgccagatc tacgacgcgg ccggcaagtt cgtcggggtc gttggcgccg
    18241 ccatgagcca ggactacttc aacggcgtct accgtgacct gcagatcttt ccggacgatg
    18301 ccatcctgat cgtcgatgcc acccgcggca cgctccaact gcgccacccc ccgaccgatg
    18361 ggcaggtcgg tgcgcccctg gggccgtcct ctgccctggg actggatgca aaccggcgca
    18421 gcgcggtgag catcgagggc gacgagacgc ccgccgcccg cctcaccgcc ttccggcgtc
    18481 tggcagacta cccgctggtg gtgggcgcca gtcgcccgct ggagcgcgta ctggccggct
    18541 tcagcgacaa ttttcggcgc atcagcgccg gtgcggccct gctgatcgcg ctcctcggag
    18601 tgctcgcgct gctcctgcac cgggacagcc tgcggcgcga cggggaccgc caatccctgg
    18661 ccgaggtcaa tgcgaatctg gaagagcgcg tccgtacccg cacggccgag ctggagcagt
    18721 ccaatcggga actggtcagt ttcagctatt ccatttccca cgacctgcgc gctccgctac
    18781 gggccatcaa cggctacgcc catctgctcg acgaggaata cggcgaccgc ctcgatgaag
    18841 gcggccacgc ttacctggcc cgtctgcgca aggccagcct gcgcatgggc gaactgatcg
    18901 acgagctgct gaaactcgcc aatgtctcgc gccagcccct gcacatcgaa ttgaccgacg
    18961 tcagcaccat ggcacgggag atcctcgacg aatttgccgc ccagtcatcc gggcgcgtcg
    19021 tccacatcca cgtggaagac ggtctcgcca ccgaggcaga ccccaatctg ctgcgcacgg
    19081 ccctgcagaa tctcctgggc aatgcctgga agttcacccg cgacagccag cccgccctga
    19141 tccaggtggg cggccgaccc catggccagg agcggctgtt ctatgtcacc gacaacggcg
    19201 tcggattcga cctggcccac gctggcaagc tgttcgagcc cttccagcaa ctccacagga
    19261 acgctggttt tgaaggctcc ggcatcgggc tggccagcgt gcggcgcatc gtcgaacggc
    19321 atggcggctc ggtgtgggcc gagtcggcgc ctggcgccgg caccacgatc ttcttcaccc
    19381 tgccacgcag cccggccatc gttcgccgcc cgcgggagcg ggtgcaggtc tgaacggcgc
    19441 gccgctcatt cgtagcggat ctcgacgacc tcgatctcgc gcacgccggc cgggctggca
    19501 aagcgcacca catccccctc ccgcgccttc agcaaggccc gcgacagagg cgcgatccag
    19561 ctgatgcggc cttctgacgc attggcctcg tccacgccga caatctggta ggtctgctcg
    19621 gcgtcggcgc tggcgtcgaa caccgtcacg gtggcgccga agaagacctg ctcggtgttc
    19681 tcctgctcgg acggcgtcac cacggttgcg gtttccaggc gcttgaccag gaagcgcacg
    19741 cgccggtcga tctcccgcag gcgcttcttg ccgtagatgt agtcgccgtt ctccgagcga
    19801 tcgccgttgg aggcagccca gcgcaccgtc tccaccaggg ccggccgctc cacccggacg
    19861 agctggtcga gttccgcccg cagacgctcg aaaccgttgc gggtcatgta gttgcgggtg
    19921 cccggcggca ggctcggtgc gccggccggc gcatcctctt cgtcgtcgtc cgattccttg
    19981 acgaatgcct tgttcacctg gagtcccttc agtgcgcgcg ttgcgagggg ggcaatggta
    20041 ggagcgccgc gaccgcgacg caagcggccc gatcacgctc acggggcgct atgatggggg
    20101 cccgcccgtc accggagtct gccgccatgc atccgatcct gtccggcaaa cgcgtcctgc
    20161 tcacccaggc cgactgcttc atgggccccg ccctgcagcg tgtcttcgag gcggccggtg
    20221 ccgccgtgat cgctgacgac cgccccctgc tcgacccggc gctgccggaa gcgctggtcc
    20281 gtcaggccgg cgacatcgac atcctgctgg cccatctcgc ggtgccgagc ccgcgtacgc
    20341 cggccaccga cgtcgccgat gacgaatggc gcgaggtctt cgcccatctg gtggatcccc
    20401 tcccccgcct ggcccgcgcc gtgctgcccg gcatggtggc ccgcggcagc ggtcgcattc
    20461 tcgtcctggg cagcgcgtcg gccctgcggg gcatgcgcaa tgcctcgacc tacagcgctg
    20521 cccgtggcgc ccaactggcc tgggtgcagg ccctcggcgt cgaggtggcg cggagcggcg
    20581 tgcgggtcaa cgccatcgcc cagaacttcg tcgagaaccc cacctatttt cccccagaag
    20641 tccaggccaa cccggcgttc caggagcgtc tggcgcggga ggtgcccctc ggccgactgg
    20701 tcagcgcgga cgaggacgcc cgctttgcgg cctatctgtg ctcggatgac gccgactgtt
    20761 tcgtcggtca ggtctttccc gtgtgtggcg gctgggccgt ccgctgaacc cgctcgaaca
    20821 ggtcaatgcc catgcgcctg caggaactgt tgtccattga atggcccctc atccaggcgc
    20881 ccatggcggg cgtccagggc agcgccctcg ccatggccgt cagccaggcc ggcgcgctcg
    20941 gctccctgcc ctgcgcgatg ctgacgcccg atgcgctgca cgccgaactg gccgccctca
    21001 gcgcatcgac cgcgcgagcc tacaacgtca atttcttcgc ccacacgccg ccgcagccgg
    21061 atggggcgcg ggaggcggcc tggcggacgc agctcgcacc ctattaccgg acattcggca
    21121 tcgatcccgc gggcattgct acggggcccg gccggatgcc cttcagcgcg gccatcgccg
    21181 atctcgtcga acccttccgc ccgcccgtgg tgagcttcca tttcggtctg cccgatgccg
    21241 ccctgctggc ccgcgtcaag gcctggggcg cccgggtgct gtcgtccgcg acgaccgtcg
    21301 aggaggcgcg ctggctggcc gcgcggggcg tcgacgggat catcgcccag gggctcgagg
    21361 ccggagggca tcggggccat ttcctcgacc acgatctcac ggtccagtct ggcaccttcg
    21421 cgctgctgcc ccagatcgtc cgcgccgtgc aggtgccggt catcgcggca ggcggcatcg
    21481 ccgatgcgga cggggtagct gccgccatgg cgctcggcgc ggcgggtgtc caggtgggca
    21541 cggcctacct cctgtgtccg gaggccacca cttcgccgat tcaccgggcg gtcctgcgca
    21601 cggagtccgc ccggcacacg gcgctgacca atctcttctc cgggcgcccg gcccgcggca
    21661 tcgtcaatcg cttgatgcgc gaactgggcc cgctcaatcc ggaagcgccc gaattcccgc
    21721 tggcgacagc ggcggttgcg ccgcttcgcg cggcggccga ggcgctgggt tccggcgact
    21781 tctcgccgct ctggtgcggg gagaatgtgt ctggctgtcg ggaaatccct gccgcagaac
    21841 tgacccgcgc cctgattcag ggcttccaac ggcgggagac cgcgtgctga aggcccacgc
    21901 ggaggcgccc gtgccgtgtc gtgaagcgtg cggcgcctgc tgcatcgccc cgtccatttc
    21961 cagcccgatt cccggcatgc ctgagggcaa gcccgcgggc gtgcgctgcg tccagctcga
    22021 cgcggcggat cgctgccgca tcttcggaca agcggagcgt cctgccgtgt gcggctccct
    22081 gcggccgacg cagtccctgt gccgcggcag ccgcgaggag gcgctgcagg ccctcgcctg
    22141 gctcgaggcg cacacggcgc cgggctgagt ccattccgcg ccggtgcgtc ccgggcgtcc
    22201 ttcgtccatt cgccgtcatc cgtattgcgg caggaagaat aagcattccg gatcatattg
    22261 gttcgtcggc cggactgaac gtcctagact ccggcccatc tccttcgccg gacgcgctgt
    22321 cgtgccccct ccccagaccc ctgctgcgac cctcattgtc cccggctacc tgggcagcgg
    22381 cccggcccat tggcaaagct ggatcgaatc ccggatcccc gacacccgcc gtgtccgcca
    22441 cgtcgattgg gacgccccgg tgctggcgcg ctgggcccag gcggtgcgcg acgagatcga
    22501 cgccgccgcc ggtcccctgt ggctggtggc ccacagcttc ggctgcctcg ccagcgtggt
    22561 ggcggcggct gatcgcccgg acaaggtggc gggcctgatg ctcgtcgcgc ccgccgaccc
    22621 ggagcgcttc acgccccgcg gggtccgtac gtccaccggc ggcgtgacgg agcccggtct
    22681 ctccggctgt ctgccgcgcc gcctgctgcc catgcccggt ctggtcgtgg ccagcaccaa
    22741 cgatccatgg gtgcgccttt cctccgccgc ctactgggcc gatcagtggg gctgccgtct
    22801 ggcggaaatc ggcgcggccg gacatatcaa tgcggactcc ggtcacggcc catggccgct
    22861 cggtttgcgc ctgttcgaat ccctgcggcg ggcggccggt gactggccgc tgggccggat
    22921 cgatgggaac gcgcagccgc ggggcgggcg gcatggcgcc ctcgcccgcg tccgtcagct
    22981 cacccgctta gcctccggac gggaaacggg cggcggcgcg ccgtcagaac cgtcggggcg
    23041 cggcggctga cggcccctcc ccgttcagaa cggcccatcg ctgcccgttt cggggcgcag
    23101 ggcgccgcgc accacggcct cggagagctc cggtgcgcac agctcgatga agcggtaggc
    23161 aaaaccgcgg agaaagtggc ctcggcgcac ggcgatgcgc gtcacgtttt caccgaagag
    23221 gtggtcgctg ggcagcatgc gcagggcgcg gtcgcgctcg gcgtcgtagg ccatggaggc
    23281 cacgatgccg atgcccaggc ccagctccac ataggtcttg atcacgtcgg cgtcgagggc
    23341 cgacatgacg atgtccggcg tgaaaccggc ctgggcgaag gtttcgtcga tcttcgcacg
    23401 gccggtgaag ccttcgtggt aggtgatgag gggatgctcg gccacggctt cgagggtgag
    23461 cggtgtgacc gcctccagcg catgcccggc cgggacgatc accgcgtggt gccagccgta
    23521 gtacgggaag gacacgaagt cggacacgcc ggccagggct tcggtggcga tgccgatgtc
    23581 ggcgcggccg tccagcagca tggcggcgat ctcgccgggg ctggcctgat gcagcatcaa
    23641 atgcaccctg gggtatgcct gcttgaagcg cgtcaccacc cgtggcaggg cgtagcgcgc
    23701 ctgggtgtgg gtggtggcca cggtgagctg gccgtgatcc ttgttgctga actgctcggc
    23761 gatgctcttg atgttgcgcg cgtccatcag catgcggtcc accaccgcag agagctcccg
    23821 ccccggctcg gtgagcccga gcaggcgctt gcccttgcgg acaaacagtt ccaggcccag
    23881 ctcgtcctcc aggtccttga tgtgcttgga gacacccgac tgggacgtga acagggcgtt
    23941 ggccacctcg gtcaggttga agttgcgccg gaccgtttca tgaatgatgc gcagttgctg
    24001 gaaattcatc agatcaccca gttcggtgcc gcggctgcgg cagcctcctg acgttcgaac
    24061 accttgagac gggacggttg caggcgcacc gcctggcctt ctttcaggcc caggcggtgg
    24121 acgtcgtcgc ggggcaggtc cacttcgtaa agctgcccgc cggcgccatt gagcccatcc
    24181 agttcgacgc gggccaccgc gccgaaggcg aggatgcgcg acacccgtgc cggcacgccg
    24241 tcggcggcgg ccggatcgac gacgatggtg agttcgtggg gccgggcgaa ggcaagcact
    24301 tcggcgccct gccccagctc cacccgctgg cgcgacagca ggtcgtcgcc cacgtgcacg
    24361 gcctgaccgt ccacgcggcc gtggaagaga ttgaccgcgc ccaggaaacc atacacgaag
    24421 ggcgaggcgg gatggcgata gacctcgtcc ggggtgccca cctgctccac cttgccgtgg
    24481 tccatcagca ccacccggtc ggccacttcg agggcttctt cctggtcgtg ggtgacgaac
    24541 agggaggtga tgtgcagctc gtcgtggagc cgccgcagcc agcggcgcag ttccttgcgg
    24601 accttggcgt cgagggcgcc gaagggttcg tccaggagca gcacgcgcgg ctccacggcc
    24661 agggcgcggg ccagggcgat gcgctggcgc tggccgccgg aaagctggct ggggaagcgg
    24721 tcggcgaccc agtcgagctg cacgaggccc agcagttcct tgaccttggc gcggatcgtc
    24781 gcgtccgaag ggcgctggcc gcggggcttc atgcgcaggc cgaaggccac gttgtcgaag
    24841 acggtcatgt ggcggaacag ggcgtaatgc tggaacacaa agccgacctg acgctcgcgc
    24901 acgtgggtgt gggaggcgtc ctgcccgtcc agcagcacct ggccgctgtc ggccgcttcg
    24961 agcccggcga tgatgcgcag cagcgtggtc ttgccgcagc cggaggggcc cagcagggcc
    25021 accagctggc cagtggggaa gtcgaggctg acgtcgtgca gggcggtgaa gctgccgaac
    25081 tgcttgtgga tgttgcggac ctggatgctc atttcattct tccttgttag cccggccggc
    25141 ctgacgctcg acccaggtct tgatgaccag ggtgacgagg gccagcagcg ccagcaggga
    25201 ggccaccgca aaggcggcgg cgaactggta ttcgttgtag aggatctcca cgtgcagggg
    25261 cagggtgttg gtctgtccgc ggatgtgccc ggagacgacg ctgacggcgc cgaattcgcc
    25321 catggcgcgg gcgttgcaga ggatcacgcc gtacaggagc ccccacttca cgttgggcag
    25381 ggtcacatgc cagaaggtct gccagccccg tgcgccgagc acgacggcgg cttcctcttc
    25441 ctcacggccc tgggcttcca tcagcgggat cagttcccgc gcgacgaagg ggaaggtgac
    25501 aaaaaccgtg gccagcacga tgcccgggat ggcgaagatg accttcacgt cgtgatccga
    25561 cagccacggc ccaaaccagc cctgggcacc gaagacgagc acgtagatca ggcccgagac
    25621 caccggcgac acggagaacg gcaggtcgat gagggtgatg agcaggtgct tgccgcggaa
    25681 ctcgaacttg gtgatggccc acgcggcggc cacgccgaag acgagattga ggggcaccgc
    25741 acacagcgcg gcagtcaggg tcagggtgat cgcggacacc gcatccggct cgatcaaggc
    25801 cgcgtaatag accgcccagc ccttgcgtag cgcttcgtag aacaccgcca ccaggggcag
    25861 cagcaggaag accgcgaaga aggccagggc aaggccgatg agggtccact tgacccaggc
    25921 cggttcgcgc gtggcggacc tggattcgaa gcgtgccgca tggtcgaaga cgccatgcag
    25981 cgcagccgca gcgcctgaca tcagcgggcc ctcccggtgc gttgggcggt ccaggcctgc
    26041 aggccgttga tgatcagcag caggatgaag gagaagatca gcatgaccac cgcgatcgcc
    26101 gttgcgccgg cgtagtcgta ctgctcaagc ttggtgatga tcataagcgg agtgatttct
    26161 gacacgaggg ggatgttgcc ggcgatgaag atcaccgagc cgtattcgcc gacggcgcgg
    26221 gcgaaggcca tggcgaagcc ggtcaggagg gccgggaaga cggtgggcag gaccacatgc
    26281 aggaaggcct gccagcggtt ggcgccgagg ctgacggcgg cctcttcgta ttcggtgtcc
    26341 aggtcttcga ggatgggctg cacggtgcgc acgacgaagg gcaggccgat gaagatgagc
    26401 gccaccagca cccccagggg cgtgaaggcc acctgaatgc cccagggttc gaggatctgc
    26461 ccaacccagc cgttcttggc gtagagggcc gtaagggcga tgcccgccac cgcggtgggc
    26521 agggcgaagg gcagatccac cagggcgtcc accaggcgct tgccggggaa ggcatagcgc
    26581 accagcaccc acgccaggag caggccgaag acggcgttga tcaacgcagc gatgagggag
    26641 gcgccgaagg acaggcggta ggaggcgagc acccgcggcg aggtggcggc ctgccagaag
    26701 tgttcaaagc cgagggaacc ggccttgagg aacaccgccg ccagggggat cagcacgatc
    26761 agggacaagt aggcgacggt gtaacccaga ctcaggccaa atccgggcag tacacgaagg
    26821 gatcgggcgg cgctcatcgg tgtttcactc cgacctggtc gtacacgccg ccatcggcga
    26881 agtggacctt ctgcaccgca tcccagccgc ccagggcgtc ctcgacggtg aaggtccgga
    26941 tgctcggaaa gcggcgggcg tagtgcttga gcacctgagg atcgcgggga cggaaatggt
    27001 gacgggcgat gatgtcctgt ccttccgggg agaacaggaa gttcaggtaa gcctcggccg
    27061 ccttgcggct gccccgcttg tcgaccacct tgcgcaccac ggccaccggg gcggcggcct
    27121 cgatggagac ggacggatag accacgtcga acttgtcgcc ccccacttcc tggtcgatga
    27181 gggtcgcctc gttctcaaag gtgaccagca catcgcccac gtcccgttgg gtgaaggtgg
    27241 tggtggcgcc gcgcccgccg ccgtcgagca ccgggacgtt ggcgaacaaa cggctgacga
    27301 aatcccgcgc gccggcttcg ttcccggttt tctggagcgc ataaccccag gcggccagat
    27361 aggtgtagcg gccattgccg gaggtcttgg ggttgggcac gatcacctgc aggcccggcc
    27421 gggtgaggtc ggaccaatcc ctgatgccct tggggttgcc cttgcgcacg aggaagatgg
    27481 tggtggtggt gtaaggcgtg gcgccatggg gataggcctt gcgccagtcg ccggcgacga
    27541 ggcccgcgcg ggccagcacg tcgatgtccg gtgcctggtt catggtgacc acgtcggcct
    27601 ccagcccggc cacaacagac tggacctgct tgctggagcc gccgtgggac tggttgatcg
    27661 tgagcttctc gcccgtttgg cgctgccatt gggcggcaaa ggcggggttg atgtccttgt
    27721 acagctcgcg ggagacgtcg taggagacgt tcagcagggt cgtgtcggcg tgggccgcgg
    27781 tcacgccgag gagcgccgag ctggccatca gcgcacgcca cgtacggcgg ctcaatcgga
    27841 tcgaaggaaa ttggaacacg gcgactcctg gggttctgat gatttcccgg aagtctaagc
    27901 agcacccgtc ggagcgctaa ccaataaatc ctgcgttctt tattctctgg ggcgataaag
    27961 aatggagatt ttaattctcc gctgttcttt tccggcacca ttcagcgtgc catcgcctca
    28021 agcgtctgca cctccggacg cccagagccc cggtcggttt cccgaccggg gctctgggtg
    28081 aaccaggttc atgagacagg aggcgacgag atcttccaga atcatccggt gggtcttgcc
    28141 gtaagtgcca agtgaaaaag gccccgccgg tttcccggcg gggccttttt cgtattgcta
    28201 cggtcgaccg gcaaaagccg gccatgatcg caacttacat ttcaaccgtt tcagccactt
    28261 ccacgaaggc cgggatctgg tcgaagttca tgtagcgata cacgtcggca gccttggcgt
    28321 tcaccagctt gatctgctcc atgtactctt ccttggtcac gatacggccg gccagggcgc
    28381 acatggcggc cagttcggcg gagccgaggt agacgcgggt gtcgatgccc agacggttcg
    28441 ggaagttacg ggtagaggtg gagatcgcgg tggaaccctt gcggatctgc gcctggttgc
    28501 ccatgcacag ggagcagccc ggcatttcca tgcgggcgcc ggccttgccg aggacgctgt
    28561 agtagccttc ttcggtcagg atcagggcgt ccatcttggt cggcggggcg atccacaggc
    28621 gggccgggat gtcgctcttg ccgtccagca ccttgccggc cgcacggaag tggccgatgt
    28681 tggtcatgca ggagccgatg aagacttcgt cgatcttgtc gccggcgact tcggacagca
    28741 ccttgacatc gtccgggtcg ttcgggcagg ccaggatcgg ctcggtgact tcggccaggt
    28801 cgatttcgat gactgcggcg tactgggcgt tggcgtcacc cttcagcagg acgccgttgg
    28861 cgatccagtc ttccatggcg gcgatgcggc gcttgagggt gcggggatcc tcgtagcctt
    28921 ccgcgatcat ccacttcatc agggtgatgt tggaacgcat gtactcgacg atcggctcct
    28981 tgttgagggc gatcgcacag gcagcggcgg aacgctcggc agcggcgtcg gacagttcga
    29041 aggcctgttc gaccttcaga tccggcagac cttcgatttc gaggatgcgg ccggagaaga
    29101 tgttcttctt gcccttcttc tcgacggtca gcaggccggc cttgatggcc cacaggggga
    29161 tggcgttgac cagatcacgc agggtgatgc cgggctgcat ctggcccttg aagcgcacca
    29221 gcacggattc gggcatgtcc agcggcatca cgccggtggc ggccgcgaag gcgaccagac
    29281 cggagccggc cgggaaggag atgccgatcg ggaagcgggt gtgggagtcg ccaccggtgc
    29341 cgacggtatc cggcagcagc agacggttga gccaggagtg gatcacgccg tcgcccggac
    29401 gcagggccac accgccgcgg ttggcgatga aggtcggcag ttcgcggtgc atcttgacgt
    29461 ctaccagctt gggataggcc gcggtgtggc agaaggactg catgaccatg tcggcggaga
    29521 agccgaggca ggccagatcc ttcagttcgt cgcgggtcat cgggccggtg gtgtcctggg
    29581 agccgacggt ggtcatcttg ggctcgcagt aggtgcccgg caggatgccc ttgccttccg
    29641 gcaggccaca cgcgcggccg accatcttct gggccaggga gtaaccggtg cccggatcgt
    29701 tgggctgctg gggcaggcgg aacagggtgg agaccggcag gcccagggct tcacgggcct
    29761 tggaggtgag accgcgaccg atgatcaggg ggatgcggcc accggcgcgg acttcgtcga
    29821 ggatgacgag ggtcttgagc tgggattcgg cgatggtggc accgttcttg gtggcggtga
    29881 ccttgccgga ggcggcgtcg atcttcagct cgatctcgtc gcccatgtcc atctggccag
    29941 cgtcaatctc gatgggcagc gcgccggcat cttccatggt gttgaagaag atcggggcga
    30001 tcttggagcc caggcacaca ccgccgaagc gcttgttcgg gacgaagggg atgtcttcgc
    30061 cggtgaacca cagcaccgag ttggtggcgg acttgcggga ggagccggta ccgaccacgt
    30121 cacccacgta agcgatcagg ttgcccttgg cagcgagggc ttcgagctgc ttgatggggc
    30181 cgcgggaacc cggctcatcg gcttcgatgc ccggacgcgg gttcttcagc atggccaggg
    30241 cgtgcagcgg gatgtccggg cgggaccagg cgtccggcgc gggggacagg tcgtcggtgt
    30301 tggtctcgcc ggtgaccttg aagacggtca gcttctggac ggcggggact tcgggacggg
    30361 aggtgaacca ctcggcgtcg gcccaggact gcatcacggc cttggcgttg gcgttgccgg
    30421 ccttaccgag ttcggcgacg tcgtggaagt agtcgaagac cagcagggtc ttcttgaggc
    30481 cttcggcggc cacggcaccc acttctgcgt cggcgagcag gtcgatcagg ggcttgacgt
    30541 tgtaaccgcc gagcatggtg cccagcagtt cggtggcctt ggcgcgggag acaagaccgc
    30601 aggccagctc gcccttggcg accttggcga ggaactcggc cttgaccttg gcagcgtcgt
    30661 ccacgccggc gggaacgcga taggtcagca gttccaccag gaaggcttct tcgccggccg
    30721 gcggattttg cagcagcgca accagatcct gggcttgctt tttggacagg ggcagcggcg
    30781 ggatgccaag ggcagcgcgc tcggcaacat gctggcggta ggcttcaagc attggtatgt
    30841 ccttcctaga cggtgggttg tgtatgaagt gattgctgac ttgagcgacc ggccgctggg
    30901 gcccgatgcg cctctcccga tgtgaccgga ccttttggaa catcatgcgg tcctgagtct
    30961 tatatatatt atattttact tcttccccgg atgtgcgcct gcacaattca aaaatctgtc
    31021 cggtcccggc tgtcccacca atcggggccg gccggtacgg gaattgccgg atgcaacagc
    31081 ttcttcattc gcggagccga tcatggtacg cctttgacga cgggacgggg atacggcagg
    31141 gagcggctca gccggtcaga acactggcca ggaccgcttc catggcgccc atcgtgaccg
    31201 gcttgatcaa caggtcgtcg aatcccgctg ccaggaggcc ctgcttctcg tcgggcagcg
    31261 catgggcagt ataggcaatc acgggcaggc tgctgaagac cgcgtcgctg cggatgcggg
    31321 ccagcacctc ttccccggac atgccgggca tgctgatgtc gagcagcacc gcgtcgtagc
    31381 ggttcgccgc gatcagggcc agggccgtag caccgtcggc ggcctggtcg gacgtgatgc
    31441 cgagacgctt ggtgaaggcc gcagccagtg tacggttgat gggattgtcg tccacgatca
    31501 ggacatgttt gtcctggaat gtcagggggc tgctcatctc ggggaggcgg tcgggcgtgc
    31561 cgttgtggcg gcatcgcggg gaagtaggag ggtgaaggtg gagccgacgc ccggttcgga
    31621 gcgcagggac agttcgccgc ccagcagatg gacgaactgg cgggcgagtg ccaggcccag
    31681 acctgttccg gcctgttcgc gcgtgacgaa ggcgtccacc tgcctgaact tgtcgaagat
    31741 cacgtcctgg gcgtccttgg gtatgccgcg accggtatcg gagaccgcca gcgcgatccg
    31801 ctcgccgtcg gcatggatgt tcagatcgat cctgccattg tcggtgaact tgatcgcgtt
    31861 gttcaagaga ttgttcagga tctggcgaag acgcagcggg tcggtgtgga ggcgctccgg
    31921 cgtaccggcg gcgatcgtgc attcgagttg cagccccttg gcctgggcgt gaccacggtg
    31981 ggccgcgcaa acctcctggg cgaaggtcgc gaggagaacg tcgctgaggt tgagctccat
    32041 gcgccccgcc tccaccttgg ccagatcgag cagatcattg accagatcca gcaggtgctg
    32101 gccggaggac tcgatcgtgg cggcgtactc ctgatgggcg gcgtcggtgg catcgagctt
    32161 gagcagttcc gcaaagccga ggatgccgtt gaggggggtc cgcagttcgt gggagatgtt
    32221 ggccaggaac tcggacttca tgcggttcgc ggcctcggcg ctctggcggg cgtcttcgga
    32281 tcgcttgaag gactcctgca cgcggtcggc catgctgttg aaggattcgg ccaggtgtcc
    32341 catctcgtcg gtggatttca cctccagccg gcggctcagg tcccccgcct ggaacccgtc
    32401 cagcccttcg atcatgcggg tgatgcgacg ggtgatgaac tgggccatcc agatcgcgac
    32461 ggcgatcacc accgccacca tgacgagcgt ggagatggcc agggctccgg cggtctgggt
    32521 cagattgtcg ttgatggcct ggctgatgcc ggcgcgcagc gtctggaatt ccttgtcctt
    32581 ggcgtcgatg gtcaggccga tgcgtttggc ggattcggtg gcggcacgat ggaagtcatc
    32641 cacattggcg ccgatggtga cgattccaaa accctggggc gacgcggcat acggtccggt
    32701 gtaatacgga atggcggccg ctgtggtgag cttccacagc ccggaaaaga agatcacgaa
    32761 cgaaccggag ccacccttct cggtgagcag cttccagccg gaacattgag gcgagaagtt
    32821 caggtagcgg cagtcgagcg ccaccgtccc ggccttgacc agggcgcccg caggcttctt
    32881 cttcagggac tgatcgtcgt agggcttcac cccttcaagg aaggcctgtg acgattttcc
    32941 ggaggactgc caggcttcgt acagggactg gtccatccaa ggcgtaaccg gctccccggt
    33001 ggccggatcg tagccgggaa tgaagtagtc gcgcgggtgg gaaatggcac ggccacgatg
    33061 gtcccacatg aaggcatagt tgccggcgat ggcgtcattg atcggcgtat agcgcgcatc
    33121 cgtcgggctg atccggtcgg tgaactggcg gatgtggtcg tggtcgaggg ccagggtcac
    33181 gtagccggtg atctgtcctt gacgcaccac cggcatggcc caccgcacga tgccgcgaaa
    33241 atggcgcccc acgggattct cggtcccggc gtaggccgac gcttcgggct tgaacggctc
    33301 gcccgccttc tccgcagacg ccggcagata ggggccgatc acgtggctct tcacataggc
    33361 gccgacgaca tccgacacat ggatctcgcc gggcttgagc ttctgcagtt caggccagta
    33421 atgctccgcc cggatgaagg tctcgcgggc gtcgctgacc tgacgcagcc ccggcgtcat
    33481 cagcgaaccc tgggtcacct tgacacgctc ccgaccggac aggtcgatga agctcatctc
    33541 cacgaagagc gggcgcatct cccgatggcc gaggaactcc ggcgggcggg cgctgaaggc
    33601 tttggcgttg tcagagaggg cctgcccggc gtcggccgca agggattcgt cccaggattc
    33661 cggtgctgcg ggctcccagc gtttgccgtc atccgcgagt ttccaggggc cgtgggaaaa
    33721 taccgggcgc tgccgatgcg cgaggaagcg tcggtagctt gcgtcaccgg gctccagttc
    33781 ggcggcttca cggatgtccg cgtcccggtc gtaaaggaac gccgccacgg cgcgggccgt
    33841 atcggtggtg aggcgctcca tgctttcccg cgcccggtcg tcgagcgcgt gcagcgcatc
    33901 ctgagtcgcc tcctcgccca cgctgcgcaa ggtacgcagc atcgagctgg acacgctctc
    33961 cagctggagg ccgaccgcat cgcccagctt ctcggccatt gtccaggcca ggagggccaa
    34021 cagcaccagc ggcacgacct tgatgacgat gaagatcgag atcagtttgc cccggatacc
    34081 gaatctgccc gtgttggtct gcatggtctt cccgtgtccg tttccatcca tcgcgccgtc
    34141 accaggcgcg cgcctgggtg cccgacgtgc acatcggctg tccgtccgaa atatcaagat
    34201 cggatctcaa gaaagtttga gcgccttgcc cgccgtccca ggggcgccga caatgtcggg
    34261 tcgtcattcc gctcaccccc atgtccgacc cctgcccttg cgggtccggg cgcgcctacc
    34321 cggcgtgctg tggcccctac ctggatcagt cactgcccgc gcccacggcc gaggcgctga
    34381 tgcgcgcccg ctacaccgcc tacaccctca agcgggagga ctacctgctc gccacctggg
    34441 acccggcgca tcgccccgcg caactcgatc tggcctctga cacggcaacc cgctggctgg
    34501 ggctgcaggt gaagcgtcat gagaccaccg gatccgggca ggccatcgtc gaattcgtgg
    34561 cccgctacaa ggtcgccggc cgcgcctacc ggctgcatga gaccagtcgc ttccatgtgc
    34621 tcggcggtgc ctgggtttac gtggacgggg acgtccactg acgggcggta cgttcaggtc
    34681 gccacgagct caatcacctc gccggcaaat tcgacccgct ggcccggacg gagcttggcg
    34741 cgcttgcggg tctcgatgac gccatccacc ttcacctgcc cttctgccac acgctgcttg
    34801 gcgacgccgc cggaatcgca caggccggtg gccttgagga gctgatcaag ctggatgaaa
    34861 tcgccctgaa cggggaaacg ggtggccatg gcacggacgt ccaacgtcaa aggggcggat
    34921 tgtcgcagag cctgccgccc ggcgccaatc cgcctgccgg gtcgaatgcc tgccgatcag
    34981 gtggggatcc gcagcatctg gcccggatag atcttgtccg gatgacccag catcggccgg
    35041 ttggcctcga agatcttcat gtacgcgttg gcgttgccat agtagtgctt ggcgatggcc
    35101 gacagggtgt cgccgcgtac cacggtgtgg aactgggctt ccggttccgg gtgggtgacg
    35161 gcgagctggt cgatcacctg ctccaccccg tccacgttgc cgcagcacag caggatcttc
    35221 tccttggtgg cctgatcgga cacgctgcca ctcgcggtga cggtgctggt cgcgccgtcg
    35281 aagctcacgc tcagtgcgct ggcgtcgaga ccctgggtgc cgatgtaggc aaggatcgcc
    35341 gcggcggcct tggcgttcag gtcggccagg cgttccgccg cgccggaatc ggccgccgct
    35401 gcttccacgt ccttgcggcc gaacagcttt tcaccggctt cacgaacaaa actcaaaaga
    35461 cccatgacgg actccctgcg tggtgtaaga aagtccccag cctacggcct cggacgcaca
    35521 cggcggatgt cgagagtgtg acgaatcgtg acgccgcgct caggccccca gcgggggcag
    35581 gccatgtcgg cggcgggcgc gggcacagcc ttcgtcgccg atgccaaggg ccgcgtaggg
    35641 cgcgaaatcc cggcagggcc ccggccgctg ttcgtagatc gagcacgaca cctcgcgccc
    35701 gacctcgccg ctcagggcga cgcagcgggg cggaccgtca tccgtgccgc gcatgcgcac
    35761 cagggtggcg gtgaccggca cagtcagcgc gtccggcaca ccgccgccgg gatggctggc
    35821 cagatccgac acatgaaaat cgacgcgaaa actggcgcaa caggcaccgc agctcaggca
    35881 gggatgggac atgggagctc aggtcgcccc gcagcatttc ttgtattttt tgccgctgcc
    35941 acaggggcag ggctcgttgc ggccggggcc ggccggcgcg gcacctgcgg cggcggccac
    36001 cacaacgggt tccggggccg ccagtccgag ggcacgccag gtctggacca gacgatggac
    36061 ggcgatgccc ttcgcttcaa ggatgtccag ctgttccgcg ctgtcggcca ctgcatcctc
    36121 ctcgtcgtca cccagcaggg tttcgagcag agcctcggcg atgctggtgt cttccacggt
    36181 gtcggtccat tcggtcggcc acagctcaaa gccctgttcg aatccggcgg tccattcctc
    36241 gccgatggcc gggtcgtcgt ctccaccctg ggcatagcag aacggctgcc aggcatcgtc
    36301 cgcctcatcg tcgccgatcg cgagcagaag atcgttgtga taacgcagga ccagttcgat
    36361 ggctttctga agtacggccc cggaaccgaa acctgcctcg tcgccgtcag cggtccacac
    36421 tgccggcagc cagacctcta ccgggatcgg tcccggcgct gccacgacgg ccgccaggta
    36481 gccgtccagc atctccatgt tcatgcaatc cgccgggacg tcgtcggaag ccagcagttc
    36541 ttcaagctgc tccagttctt cttcggtcag gggcatgctg acgggaatcg ggttcatgac
    36601 gactgttcct ccagttggtc gagctccacc acacactggt atagcgtctg gccggaatcc
    36661 cggtatttgc gttcgaaagg ggtgagcggc gacggcgcct cgaggggctg ccagggcgcc
    36721 ggcacaccgg tgagcagttc cacggcgcgg gcgaattccg cgatgtaaat cggccagttg
    36781 ctgcggcact ccaggcgccc acccagacgc ggcacgcacg gaaacaccgg gtgggcgtgc
    36841 cagcgccggg tgaggtgtcc gatcttcggc cacgggttgg gatagaggat gtaatggcgg
    36901 tccaggcgaa tgcccgcatc ggcgagcaga cgccagtagt ccaccagatc ggcacgacag
    36961 aacacgagat tggtcggcat cagcgcgtcc ggatagggct tcttgcggct caggcggtcc
    37021 tcggactgat ccaccccgat cacataatgg tcgggaaaag cccgcgccag ggcgatggtg
    37081 ctgtgcccta ctccgcatcc ggcgtccagc agcagcggcg cgcgccgatc ccagccggca
    37141 agactggctt caaacgcggc cacgttgtag ggcgcatagg gtttgcggaa gtccgtcgcg
    37201 gcgtgcttgt tcacgcggct ctcaagggcc tcgtggagtc cgctctgggc gctctcgggg
    37261 atgcgggaat tggcgtacat ccgccgattc tacgcggctg ccgacagcac cggcaccccg
    37321 tggcgccgca gatggccctc gaccgccgtg cagtccttca atgcgcggat ctcgccatac
    37381 aaggactccg cctgcgggaa gttgcggcgc agcaggttga gccactgctt gaggcgtccc
    37441 ggcgcatggc gggcttccag cttggcgcgt acccgccacc agaattcccc gaggagcggc
    37501 gacagctcgg cccactcggc gtcgcggtcg ggggttttgg tgccgtcagc ctgcagcgcg
    37561 cggatgcgcc gtgccagaaa ggggtcggcc accgcgccac gccccagcat cacgtcgctc
    37621 accccgctca cggcacggca gcgttgccag tcggcctcgc tccacacttc tccattggct
    37681 gccaccggca cgccgaccgt atcggcgatg cgcccgaccc attcccagtg ggccggcggc
    37741 cgatagccgt cgaccttggt gcgggcatgg accaccagcg cgtcgatgcc gccgtccgcc
    37801 agggcgcggg cgcagtccag cgtccgcccc gtatcggcga tgcccaggcg catcttggcg
    37861 gtgaagggaa ttcccttggg cacgctcgcc cggaccgcgc aggcaatggc gtggagcagt
    37921 tccggctcgt cgaggagggc ggcgccgccc cggtgacgat tcaccgtggg cgccggacag
    37981 ccgaagttca ggtcgatgcc cgccggatgc agacgcacca ggcgcgccgc gctcgccgcc
    38041 atacgctgcg gatcggagcc cagcagttgc aggtggaccg gcgtgccgga cgaggtccgg
    38101 ctgccgactt tcagctcggg cgcaatacgg gtgaagctgc gccctggcaa cacggtcgcc
    38161 gagacccgca gaaactccgt cacggcatgg tcgtagcccg ggatgcgggt caacacctcg
    38221 cggaggacat cgtccgcaag gccttccatc ggggcgaggt aaaggcgggg catgggcaat
    38281 cgggtccggc gtcacggccg ggcgctgcga gaacaggggc ggcactttac cggagttggc
    38341 tcgcggactg aaggggccgt tccgtcgcgc gcacgggcct cggcaaaggg gtatagtcac
    38401 ccgcctctgt gtatcccgac cgtccgcctg cgcaccctgc ccatcatgcg ccgaattgac
    38461 ttcacaaatc gccgggaatg gctgaccctg gcgctccccg ccctgctcct cgtcgtcgcg
    38521 gcctttgcgg tggcgtggaa gtttgtccag cccgctccgc cccgacagat cgtcatgagt
    38581 accgggcccg ccggcggcgc ctaccagtac tttgccgaac gctatcggga acgtctggcg
    38641 cgggatggca tcgaactggc cctgcgtccg tccaccggcg ccgtggagaa cctcaaccgc
    38701 ctcaagaccg tggatgaggt ggacatgggc ttcgtgcagg gtggcgtggc cgacgagccg
    38761 gagtcggacg atctggccac cctgggcagc atgtacctgg aacccgtgtg ggtgttcttt
    38821 cggggcaagg ccgatctgga ccgcatcgac cagctgaagg gacgccgcat cgccgtgggg
    38881 gccccgggca gcggaacgct cctgttcgca ctccagttgc tgtccgccaa cggctttgcg
    38941 accgatgatc ctcatttcat ccacctgggc gggctggagg ccgtcgaggc gatccgctcc
    39001 ggtcaggtgg atgcagtatt tgccgtgggg gccccccagg ccccggccat ccaggccctg
    39061 ctcgccgagc caggcctgtc cctgctcaat ttcgcccagg ccgacggcta cgtccgccac
    39121 tttccgcacc tgtcccgcat cacgctgccc cgcggcgcga tcgacattgc ccaggatcgc
    39181 ccgccccgcg acatccacct cctcgccgcc accgcgaatc tggtggtgat ggcggacctg
    39241 catccggcga tcgtcacccg cgtgcttcag catgccgcgc agatccatgg ccagcccggc
    39301 atgttcatga cgcccttcgc ctttcccgcc aaccaggacc gctccctccc gctgcatcct
    39361 tctgcgcggc gcttctacga aagcgggccg cccttcctgc agcgctacct gccgttctgg
    39421 ctggcggtgc tgatcgatcg ccttatcgtg atgctggtgc ccatcgtggc ggtggtgatt
    39481 cccctcttca aggtgctgcc cgcgctctac aactggcgca tgcgctcgcg ggtgtatcgc
    39541 tggtacggcg aactcaagtt cctcgaaaat gagatcggcc agcaaagcac ccacacaccg
    39601 gaagtgctgg atgacttcat ccgccgcctc gatgggatcg aggaccgcgc cagtcgccgg
    39661 aagctaccgc tggccttcag caacgagctc tacaccctgc gcgaacacat caatctggtt
    39721 cggcagcgcc ttgaacgcct gcaggcacgg gctcaagtcc ctgcggcgcc gaccgtttca
    39781 tgaatccatc cttgccgtcc ggaacctcgc ccggatccac attgaccgcg atctgcccat
    39841 gagcccccac ctggacctca ccgaacccta cgccccgctc gtccgccgca tcgaagcgga
    39901 ggccaccgtc accaccgacg gcaatctgcg catcgaccac tttctcaacc accgcgtcga
    39961 acccgcctac atcgcagagc tggggcacga gctggcccgg cgcctgtcct acttccagcc
    40021 caacgtgatc ctcaccgccg aggccagcgg catcgcgcca gccttgatcg tggcccaggt
    40081 gctgagcatc cccatggtgt acgccaagaa gtactcctgc gacgtggagg gccccgccat
    40141 cagccggatc gtggcctcgc cgggacgggg cgccgaagtt cgtctggccc tgtcgtcccg
    40201 ctacgtgcgc agcggacatc gggtcgtggt cgtggatgac ttcctgtcca acggccgcac
    40261 ggcggcggcc ctcgtggaga tggcccgcga ggccggcgca gaggtgctcg gcgccggctt
    40321 catcgtggag aagcgcttca atcgcgggcg tgaacatatc gcagcaatga acgtccccat
    40381 cgccaccctc gcccagatcg aattcctgca cgacggccgc gcctgcctga cccagccgcc
    40441 cgccgcctga gcaccgccat gtcgccccgg cagatcctgc gcagcctctt cgacgccgcc
    40501 ctcgccgcgg cccagcccga gcgcgtcgtg ccgcttcatc tgccagacgg cggcgccctg
    40561 ccccgcgggc gcgtcatcgt catcggcgct ggcaaggcct cggccgccat ggcccgggcc
    40621 gtggaagatc actggccggg tgatccggct cgactgtccg ggctggtcat cacccgctac
    40681 ggacacgggg tgccctgcag gcatatcgaa atcgtcgaag cggcccaccc ggtgcccgac
    40741 gaacgcggct acgccgccgc ccggcgcatg ctcgaacaca ccgtcgcgga gcttggcccg
    40801 gacgatctcg tgctgtgcct catttccggc ggtggctctg ccctgctggc ggtgccagcc
    40861 gcgggcatca ccctggccga caagcaggcc atcggccgcg gccttctcgc cagtggcgcc
    40921 accatcggcg agatcaactg cgtgcgcaag catctctcgg cgatcaaggg cgggcgcctg
    40981 gcgctggcct gccagccggc acgggtgctg accctgctga tatccgacgt gcccggcgat
    41041 gacccgtcca tcatcgcgtc gggccccacg gtgggagacc cgaccacctg cgccgaggcc
    41101 ctgaccgtcc tcgacaagta cgccatcccg ctgcccgcgc atgtccgtgc gcacctcgaa
    41161 tccgctcgcg gcgaaagccc caagcccggt catccggcct ttgcccggtg cgaaacccgc
    41221 ctcatcgcca cgccgcagca ggcccttgaa gccgccgccc gaagggcccg cgagctgggg
    41281 ttgagcgccc acattctgtc ggacgccatg gaaggcgaat cccgtcacgt cgcccgcgtg
    41341 catgccgccc tggcgcgcca ggtggcggac cgcggccagc ccttcgtggc accctgtgtg
    41401 ctcctctccg gcggcgagac caccgtcacc gtcagcggca agggccaggg aggccgcaat
    41461 gtggagttcc tcgtggccct ggcgcaggcc ctcgacggcc acccgcgcgt tcatgccctg
    41521 gcggccgaca ccgatggtgt cgacggcagc ctcgacatcg ccggtgcctg catcgatccg
    41581 acgacgccgg cccgcacctt agcgctggga cggcgtctgg ccgacgtgct ggcggacaac
    41641 gacggtcacg ggctgttcca ggccctgggc gactcggtcg tcaccgggcc cacgcagacc
    41701 aacgtcaacg atttccgtgc catcctgatc gacgcgccgg acgcggcact ctgagcgttc
    41761 gcgccccgac gccggccgcg gatcagcgga aatgcacccg cacttccgga ttgccgggca
    41821 ggccatcgta acgcaccaca atgtcgagac cggatttcgg cgccaggagg cggctgaagg
    41881 agcccaggga cagcagatcg ccccgctgca gcggtgtgcc ggcggcggcg agatcccggg
    41941 ccagccagat cgccgcattg agcggatgac ccaggatggc gctgccctgg gcgctgtcca
    42001 gcacggcccc cgtgccgtcc cgtaccgaca cagtcattga cgcgagcgcc tccgcccgga
    42061 gaccgcccga ccgggccggc acgggcgtcc cctgcacgcc aagacgcgcg cccacattca
    42121 ccagggccag catcggccca ctcagtgcgc ccggatcgcc gaccatcaga tccggcaact
    42181 ccacgaacgg gatcaccgcg gagagggcct gcaaggccgc gtcgggcgat ccggcccgcg
    42241 ccagtgcatg aggatcggcc acttccacca gcaggtccgc ctcgaagacc gggcggatac
    42301 cgaacgcggc cggcacctgg gcaccgtcgg gtagcagacc ggcacggaac aggcgacccc
    42361 gcagcggggc cccgtagccg aagcgctgct gtaccgccgg gttggtgagc cccgccttgt
    42421 gcccgaccgg tgctccccag gcctgggcca ggaagcgcac caccttgtcc cgtccgcaca
    42481 gaccgtcctc catgcgcgtg agaccgggca ccgcgtgtac gggggttcgg ttcacgatgg
    42541 cggctgccac cgcagcggcc tccacgtcag caggacattc cgcgcgggcg ccggacacca
    42601 gcagcgcgcc catcagtacg ccggcccgcc agcgcgagga cccccggcac atctcagcgc
    42661 gccagcgggt agaaaagctg ctcattgccg atcttgtagg cagcgatggc tcggcgtccg
    42721 tcggcggagg tcagccagtc catgaagcgc agcgccgccg cccgcttgac gtggggatgc
    42781 ctggcgggat tgacgaggat cacgccgtag gggttgtaca actgcggatc gccggcgaac
    42841 aggatgccca gatcctgacg gttccggaag ctggcccagg tgccccggtc gctgagggta
    42901 taggcgccca ggcctgccgc catgttgagc gtcggaccca tgccggcgcc ggcctccttg
    42961 taccagccct ggcccctggg atcgaccccg gccgccttcc agaagcgcag ctcggccgca
    43021 tgggtaccgc tgcggtcgcc gcgggagatc caggttgccc cctgactgcc aatccgtgca
    43081 aaggcgtccg aagcatccct ggcacgcttc acgccggccg gatcggcggc cggaccgatg
    43141 actacgaagt cgttgtacat cacatcgcgt cggtcgacgc cgtagccgtc ggcaacgaac
    43201 ttgtcctcgg ccgggcgatc atggaccagg accacgtccg catcgccccg ccgccccacg
    43261 tcgagggcct gccccgtgcc cagggccacg accctgacgg cgatgccgga gtccttttcg
    43321 aagatgggca ggatgaagtt gaagagaccg gattgctccg tcgacgtcgt cgacgccacc
    43381 acgatgcttt caccggcctg gaccgctgcc gtggacaccg ccagtcccag caggaccgaa
    43441 cggaaaaatt tcagaaggcc atgcgccatg gcagctctcc ctggatgtaa agacgtgccg
    43501 ccgggctggc gggacgcgcg aagaaacgtt ggaccggcgc atgttcgcac acgcgaccgg
    43561 cgtccatgaa gaccacatcg tcggccagcc gggtggcctg accgaggttg tgggtggtca
    43621 tgagtatgcg cgtgccgtcc gtacggatct cgcgcacgat gcgctcgatg gcctccacgg
    43681 acgaggggtc gaggctggca gtgggttcgt cgagcaacag cagttggggg cgggtcaccc
    43741 acgcccgcgc cagcgccagc cgctgccgct cgccgccgga gagcccccgt gcgctgtccg
    43801 tcaggcggtg ggccagaccg acccgctcca gcaccgcagc ggcccggcgg cgacgctcgg
    43861 tcgcagcgat tccacgcggt gtgagggcca gcgccacatt gcccaggatc gaatcgcgga
    43921 acatgcgcgg ctgctggaag accatggata ccccggcacc cggcgtcgct gcgccgttcc
    43981 agtcgatcag ccccgcgtcg ggtcgaagca atccgcacac gtgccgcagc aggacgctct
    44041 tgccggcacc gttcggtccc agcaggacgg tgatgccctc gccgtccagg cgcaggttca
    44101 ggccgtcgag gatcgtgcgc ggccccgccc gccatacgag gtcgctcacc gtcagcgcaa
    44161 acatcggcgg acgtcctcag gcatagcgcc ggaatgccca ggtgcgcagc atataggcgg
    44221 cagcattgat gagaaggatg accgccagca ggacgacgcc cagggccagc gcgaggggca
    44281 ggtcaccctt gctggtctcc agggcgatcg ccgtagtcat gacgcgggtc gaccggtcga
    44341 tgttgccgcc aacgatcatc actgcgccca cttcggacat ggcccgtccg agcccggcga
    44401 gcacggccac cagcagggaa tgccggcagt cgtgcaacag cagggtcacg ctgtcgatcc
    44461 agcgaaaacg catggccgcg agctcttcgg cgtaatcccg ccaggcatcc tccaccacct
    44521 ggcgggcgat ggcggccatc agcgggatga ccaggagggt ctgggcgatc accatggcca
    44581 ccggggaata catcagccca agatgaccga aggggccgga gcgggacagg gccagataga
    44641 cgaccacgcc caccaccaca gagggtaagc ccatgcaagc gttgaggaag atgaccagtg
    44701 cctgacgccc cggaaaacgt cccaccccca aacatgcgcc aagcggcagg ccgacgagcg
    44761 tacccgccag caccgccagg ccactgaccc gcagcgacag gacgacgatg tccacgacct
    44821 gcctgtcgaa accggtcagc aattgaaagg cttcgagaaa cgcctgcccc atcccgctca
    44881 tccaggtgcg tgcgccctca tccaccgccc cgacgcatgg ccagatcgat gcgatgcagc
    44941 aaggttcgcc gttccgcacc attgcccgca agaaagcgca gatgcgcggc cagggccggc
    45001 aggcggatca ttccgatgac ccgatccggc agcggtgtca gggacacatc cagcaccgcc
    45061 ccgtcgcctt ccacacgaaa gcgaccggcg gtgacagtga tcgtccgatc cgtctcggcg
    45121 agccgcagcg cccgttcgaa ctccgtcatg ctgatgctca tctcgcggga aaaaccctcc
    45181 ggcgcaagaa cgttctcgac catgcgatcc ttcagccccc ggcgatgggc ggccagatgg
    45241 cgagttcgtc cccctcggcg agcacgtgac tgcgccgtgc gttcggctcg atgtaatggc
    45301 cgttgaccag caccagatga gcgctgcgct cgggaatccg gtagcggtcg atcagatcgc
    45361 ccaccgtcgt cccgggcgcc aggtccaact cgacccggtt gctgcgccgc tcggccggca
    45421 gatagtcggc gagcgacgcg aacagcttga acgcaatctt catgcgaagg ctgcgacggg
    45481 ctgatgacgg gcgatggcgt cggactgggt cgccgccaga tacgcttcga agaagcgctg
    45541 cggcgcgtgc agcaggacgt ccttccaggg gcccagccgc aaacgaccct gaatcatgcc
    45601 gcgcagggca cccacatgct gggtcatgcc aatggaggtg gcgccgatca ggacgtcttc
    45661 cttgaactgc aggctcaggt agcggaacgc cacctcgtcc acctgctcca ccccctgccc
    45721 ttccggatcg ccccaccact ggccgaagga cgtggagata agccccaggg tgtcgagtac
    45781 gttgatggcc agcacgccgg tcagccgcgc ctgaccgccg gccatgttga tggcggccac
    45841 ccgcgcctgg tctgccgcat tgggctggat ggcggagacc aggtgggcgc cggtgaacag
    45901 atccggagcc tcggccacat cgcccgctgc aaagatgccg ggcaccgagg tctccatgcg
    45961 tgcgtcgacg aggacgccct tggctacgtg aaccggagtc gcttcgagga aagagacgtt
    46021 cggcgcgaca cctgccgcca cgatgaccag atcgcagggc agcttgtcac cggtggacag
    46081 gctcaccagc agcggcgcac cgtgaccgtc tccatggtcg atccgttcga ccccggccga
    46141 agtcaccacc cggatgccct ggttctcgac ccagcgcttg atcatgccgc ctgcctgggg
    46201 cgtcatcatc cgcggcacca tccggtcgcc catttccacc acggtcagct gcacgccccg
    46261 tttggccagg gcttccatga tgatgcaccc gatgaagccc gcccccagct gaaggacacg
    46321 ggagcccggc ttggccagtc gggcaatcgc gcgagcgtcc tccagggtcc agcaggtctg
    46381 gacttccggc agatcgacac ccgggatcgg tggccgcacc ggatgagaac cggtcgccac
    46441 cagcagacgg tcgtaatgtt cgaaatggcc atcggcgaag agcacccgtc gatccgccac
    46501 atcgagggcc accgcacggc cactcagttg ccggacgcca agcgcgtcga aatgccccgg
    46561 cgtcttgcgc agccaggtgc cgggctcttc gatgttgcct tccagcagat agggaatggc
    46621 catgcgggag tacggtggtt cgttctcgct gccgaccatc acgatctcac cacgggggtc
    46681 ggccttgcgc agcgcttcga cggcgacgac gccggccggg ccgttaccca gaatcaggtg
    46741 tttcatgggg aattccttcc gctcgtgcac agacgcgcgc tcgccttttg gcaagcggcg
    46801 ctgcgatgta tacgtgaaaa ggcctctccc gcccggcggg gcgggagagc cagaacctgg
    46861 agatgctgcg gcttacaggc ccaggcgctc cagggtcgac gccgtcggga cgccttcctc
    46921 gctccagccg cgcagggcgt agtactcggg cagcatcttg tcgatgccgc tgacgagccc
    46981 cttggccgga ccggaatccg cggcttccgt cttcaggcgc gggggcaggt cgtcgtcctt
    47041 gtgggtcaga ccggccgcca ggttgaacat gcgctccatg ttccagatgc gctcgcccat
    47101 ggcattgagc ttgtccatgg accaatcacc ctcgcaggcc gcctggacct ggggctgcac
    47161 atcggccacc gtccaggcaa aggacgtgaa gacacagatg cccgaggcgt cgaacaccgc
    47221 ggtggcgtcc tggaaggcct tgacgagttc gggtttgccc tccgtggtga gcggatcggt
    47281 cttgaccgga atgcccagca cttccgacgc cacggtgtag gaacgcagat ggcaggcgcc
    47341 ccggttggac gtggcgtagg tcaggcccat gccctggatg ccgcgcgcgt cataggcggg
    47401 gaattcctgc cccttgacgg tcatggacaa ttcgggatgg ccatacttgg tgcacaggcg
    47461 cttggagccg agaccgatct ccttgccaaa gccttcgccc ttcgcggtga tctccgccag
    47521 gtgcgccagg gcggcggccg acccgaacgg tgcctcgacg cccagttgct ctttggtcag
    47581 gagccccatt tcgaagagct ccatcacggc gccgatggtc gccccaaagg aaatcggatc
    47641 catgccctgc tcgttgcaca gcaggttggc gtactggagc gcttccagat cacccactcc
    47701 gttggcggca cccagtgccc acgcggcctc gtattccagc ccgccggaag caccccagta
    47761 cttgggattg ttcaccaccg tgaagtgacc cttgtcgatt tcggaaatgc gcccgcaggc
    47821 gatggtgcat ccgaagcagg ccgcattggt caccagatgc ggtttgccgt ccgtgggacg
    47881 tttttcgtgc atcgcttcgg cggagatctt gcccgcgtcc tcgaactgga cgttgcgatg
    47941 gttgcgcgtg ggcagcgcac cgatctcgtt gatgacgttc atcagcacct gggtgccgta
    48001 cttgggaagc ccctgacccg tcacggcgtt gtccgcgagg atcttcttct tttcggccgt
    48061 gatcttcagg aaggccttca tgtccttgat gcccgacacc ccctgggtac cgcgcaaggc
    48121 gaccgccttg aggttcttgg agcccatcac ggcgccaacg ccggagcgtc cggccgcccg
    48181 gtgcaggtca ttgacgatgc acgagaacag cacctgattc tcgccggcca gaccgatgga
    48241 tgacacacgc accagcggat cctgatgggc atgcttgatc atctcctcgg tttcccagca
    48301 gctcttgccc cacagatggc tggcgtcccg caactcggcc ttgtcgttct cgacatacag
    48361 gtacacgggc ttgggcgagc gtccttcgaa gatgatcatg tcccaaccgg caaacttcat
    48421 ctccgcgcca aagaagccgc cggagttgga gcaggcgatg gcattggtca gggggccctt
    48481 cgtcaccacc gtatatcggc cgcccgtgga ggcgaccgta ccggtcagcg ggccggtgga
    48541 catgatcatc ttgttctcgg gagagagcgg atcgactttg ggatcgattt ccgagaccag
    48601 gtatttggtg gcgaggccac gggagccgag gtactcgtcg gcccatgcca tgttcagcgg
    48661 ttcttccttg caggtgccag cggtcaggtt gacgcgcagg atctttctgt tccagcccat
    48721 gatgcgtctc cttccttatt ccgtcacggc cgtattggcc tttgccgccc aggcccgcat
    48781 gcgatccaga ccggtccagt ccgcatcgat gtaggtgatg gcccccgtcg gacaggccga
    48841 cgcacaggcc ggatcgccgc cacacaggtc gcacttctgg accttgccgg tttcctggtt
    48901 gtaattgacg gtaccaaagg ggcacgagat ggtgcagacc ttgcacccta cacagacatc
    48961 ctcatggact tccttcgcgc cggtcgtgcc gttgacgcgg atcgcgtcca ccggacaggc
    49021 gttcagacac cacgcttcgg tacattgggt gcaggtgtat ggcaccttgc gaccctccgt
    49081 ctcgaagctg aagaccttga tgcgggactt ggacggattg atgatccccg tatgctcgac
    49141 cgaacacgcc atttcacact gcaggcaacc ggtacatttc ttgggatcaa tgtgcagtga
    49201 tttccacatt gaaaacttcc tccgtgatga tggtgctacg tgccgggcgg tgcgatcaca
    49261 tgtggatcgg agctcacttc gaggtggcat gtactgccgc cgacaagggg tttctcccac
    49321 cgggcgcacg tccacaccgg taggcgcggc gtgcgtcgat atattgaaga atatcgtggg
    49381 aggagtgctt gtcaatgcgc gccgcaccat gggctgcgcc gattcacgat ccggacggat
    49441 atgggatact ccggcccgac atgcggcgac gtgccgcgct gacgaaggac taccacaatg
    49501 tcccaagatt ctggctgtgc tgccccgtct tcctcgcctg catccctgac ggtcgatgcg
    49561 gcccggcgcc tcatctttga ccgcctgacg cccgtcggcg gttacgagcg catcgccctc
    49621 cgcgccgccc tgggccggat tctggccgaa ccggtgatcg ccccgtgcaa cgtgcctgcc
    49681 cacgacaact cggccatgga cggctacgcc attcgagctg acgatctccc ggaaagtggt
    49741 gaggcatcct tccaggtggt tggaacggcc tacgcggggc gtggattttc gggcatcgtc
    49801 ggcgcgggac aggcggtccg catcatgacc ggcgcggtcg tgccgatggg cgcggacacc
    49861 atcgtcgtcc aggagatcgt gcagacggac ggcgaccggg tcgtcgtgcc gcccggtcag
    49921 cggcgcggcc agaatctgcg tcgcgcgggg gaggatctgg cgctcggggc catcgccctg
    49981 cccgcgggca agcgcctggg gccggccgaa ctggggctgg tggcctctct gggcatcgcc
    50041 gaggtcagcg tgacacggcg cctgcgggtg gccttcttct cgaccggcga cgaactggcg
    50101 agcatcggcc ggccgctggc acccggtgaa gtctatgact cgaaccgcta taccctcttc
    50161 ggcgccctca cccgcctcgg atgcgacctg ctggacatgg gcgtgatccg ggacgagcct
    50221 gagtccatgg agaccgcctt ccgggaagcg gcccggcacg cggacgtgat catcacgacg
    50281 ggcggcgttt cggtgggcga agccgacttc atccgcgacc tgatggcgcg tctgggcgag
    50341 gtgagcttct ggaaactgga gatcaagccg ggccgcccca tggccttcgg ccggctgggt
    50401 tcggcctgga tgttcggact gccgggcaat ccggtcgcgg tgatggtcac cttctatcaa
    50461 ttcgtgcagg acgccctgct tcatctcggc ggactcacgc cgctccctga gcgtccgctg
    50521 gtgccggcgg aatgcgccga tgccatccgc aagcagccgg ggcggcggga attcctgcgc
    50581 ggacgcctca gccgtgagga cggcatctgg aaggtgcgtc tgagcggtgc gcagggctca
    50641 ggcatcctgc gctccatgtc tgaagccaat tgtttcatcg tgctggaaga gacccgcggt
    50701 aatgtcgcac cgggcgatgc ggtctcggtc cagctcttcg acggattgat ctgagcagac
    50761 acggccggtc cgccgcccgc tttgaccggc atgcgtgaca cggcacaaaa aaagccgacc
    50821 ggcgaaccgg tcggcttttt tcagtcggac gaccggatta cttggtcagt gccgacttga
    50881 tctgctcgag ggtgctggga tcctcgatgg tggtcagatc gcccggatcg cggccttccg
    50941 ccagcgcctg gatggagcgg cgcagcatct tgccggagcg ggtcttgggc aggccggaga
    51001 tgaagtgcac gcgggccggg cgggcaatgg cgccgaggct gccgtccacg atcttcatca
    51061 cagccttttc cagctccacg accttctcgg gcgtatcgac gctggccgga tccttgacca
    51121 cggcgaaggc catgggcatc tgacccttga gctgatccgc cacgcccacc accgccacct
    51181 cggcaatggc ggaatgggac tggatcgcct cctcgatctc gcgcgtgccg agacggtgac
    51241 cagccacgtt gatcacgtcg tcggtacggc cgaggatctt gtagtaaccc gcgtcgtcct
    51301 tgatgcccca gtcgaaggac gagtagacca gcggctcctg gaacagggag aagtaggtgg
    51361 acacgaaacg cttatcgtca ccccacaccg tggtgaggca gcccggcggc agcggcggca
    51421 ccacgccgac gatgcccttc tcgttggggc cgcattctga gccgtcgtcc cggaacaggc
    51481 gcacgtcgta gccgtacacc gggaagccgg gggagccgag ctggacctcc tggcgctcga
    51541 caccgggcat cagggacagc atggcccagc cggtttcggt ctgccagtag tggtcgataa
    51601 cctggatccc cagctcgtcc atcatccact ggtgggaggt ctcgtccatc ggctcgccgg
    51661 cacagtacag ggcgcgcagc ttggagaggt cgtacttctt gaggaaggcc ggatcctgct
    51721 tcttcagcac gcggatggcg gtgggtgcgc tgaacatcac ggtgacgtcc tgctcctggc
    51781 agatcttcca ccagatgccg gcgtcgggac gcagaggcgt gccttcgtac atgatggtgg
    51841 ccatgccgtt gatcagcggg ccgtagatga tgtagctgtg acccaccacc cagccgatgt
    51901 cggaggtgga gaagaaggtt tcgccgggat tgccgcagta gatgtgcttc atggacgcgg
    51961 cgagggcgac agcgtagccg ccggtgtcac gctgcacgcc cttgggtttg ccagtggtgc
    52021 cggaggtgta caggatgtag ctgggctcgg aggattccat ccaggtcacg ggcacctgtg
    52081 cgtccaggtg cttctcgcgc agcgcagcgt agtccacatc gcgaccggcc accacggcca
    52141 tgttggggtc gaggttgcgg ttgaccatca gcaccttggc aggcttgtgt tcggccaggt
    52201 tgatggcttc gtccaacagg tgcttgtagg gcaccggacg gccgccacgc atgccggcat
    52261 cggcggagac gatcaccgtg ggcttggcat cgtcgatgcg ggtggccagc gaatgggacg
    52321 cgaagccgcc gaaaaccacc gagtggatgg caccgatgcg ggtgcaggcc agcatcgcga
    52381 agcaggcttc ggcgatcatc ggcatgtaga tcaggacgcg atcgcccttc ttgacgccga
    52441 gatcctgata gatcgccgcc atgcgcatga cctcggtgcg cagttcggca aagctgtaga
    52501 ccttttcctc gtcggtttcg gtggagatat aaaccaacgc cttgcggtcc gggtgggact
    52561 cggcgtggcg atcaacggcg ttgtagcaga ggttggtctc accgcccacg aaccacttga
    52621 cgaagggcgg gttggaaaag tcgtagatct gggacggctg cttgttccag tggatcagtt
    52681 gagcctgttc gccccagaaa ccatcccgat cctcgatgga gcgcttgtga aattccttgt
    52741 aagaagccat gggtttcctc tccctttgtt cgtatcgtga aagcactgca tgccggggcg
    52801 gtacgcggat gcgtccgtca ccttatccgt tgttgtggaa gccagccacc agcccggtgt
    52861 ctgcagcgcg tgcgagggag gcaagcggca gcccggagcg gaattcggat tctatcaatt
    52921 ccagcagcgg caagagtgtc cggatcgcat cgggcagctg accgcgcacg tccgtcgcgt
    52981 tgccggccct gacgcgctgg agcaatgcgt cgatgctgac cggcggccgc gcggctgcct
    53041 tctgcgcgac ggaggcttcg gtgacctcac cgtgaggccc gataacccgg tcgccgccga
    53101 gggtccgacc ggtcgaaagt cgctgcagcg catccgcaat gaggtgactg accgtggtcc
    53161 gcccatccgg cgagttggcg gcaatcccaa ctgtcagatg gatatggata cgctcttccc
    53221 gataggtcat caccatgtgg ttgatggcct ggcgcaggcg gaaggcaaag gctgccacgc
    53281 cgtcgatgtc cgtgttcggc gacaccacgg caaatcgtgc cgtggccagc tctgcgacgg
    53341 tgtcctcctg acgcagcttg gtggccagga tgcgcgacag cttgcggtgg atgagctggg
    53401 ccacgtgggc gccataccgg ctcaccagcg tatcgaaccc gtcgatctcg atgaccatca
    53461 cgctgacatc ggtgtgccgt cgcgccgcca gggacagttc ctgggcagcg tgatggtgca
    53521 ggtaggaggg cgtcgccagc ccggagacgg gatccacggg gctcgacgtg gccagcgcgg
    53581 cacgactttc ttccaggtcc cgctgggtgc gggccagttg ggtgagcgct tcgaggcggg
    53641 cgagcagttc gacggtggag atgctcttgg aaatgaagtc ggtggcgccg gcgctcttgg
    53701 ccttcacgcg cacttcgggc tcgtcgtcgc ccgatatgac cgccaccggc aaggtggaga
    53761 tgcgcgacag gcgcgagccg cggatgcgct ccagcagccc gtaaccgtcc agacgcggca
    53821 tgccgatgtc ggtgatgacc gcctggatgg tcggatccac catcagggtc tgccagccgt
    53881 cttccccgtc cgcttcctcg cggacctcga aacgcccccg caggtgccgg atcagggaag
    53941 cccggaccat gcgggagtcg tcgatgatca ggaccctggg cagggcctga tcgctgtcgt
    54001 cattcatgct gcgctcgctg tgaggggtgt cctcatgcgc taacggcaat caccgtgcgg
    54061 cccttgaccc gccccgcgat gaaatcgtcg aacacccccg gcagatctgc aaaaggcacc
    54121 gtgcgggaca tggactcgag atgacgcggg cgcaggtcgg tggccagccg atcccagacc
    54181 cgcgtgcgcg tcgggaagcc catgtagccg gaatccaccc ccagcagact cacgccgcgc
    54241 aggatgaagg ggaagaccgt ggtgttcagg ctgatgctct gggcgttgcc gatgctcgcc
    54301 acggtgccgg cctgtttcat ggtggacagc acccaggcga ggatgtcgcc ccccacgttg
    54361 tccaccgcgc cggcccacag gcccgcgtcc agcggccgcg tcttggccgg atcgatggtc
    54421 gaacgcaggc gaatctcggc cgcaccgagt tccctcagat aagcctcttc ctcggccttg
    54481 ccggtcagcg ccaccacgtg gtagccgaga ccggccagca tgtcgatggc cagcccgccc
    54541 acaccgccgg tggcgcccgt gacgatcacc ggaccgttgt ccgggcacag gccgttgtcc
    54601 tccatgcgca cgatgcccag tgctgcggtg aagccggccg tgccgagcgc catggcatcg
    54661 aacagggaga gccccgccgg cagcggcacc acccacccgg caggcacccg cgcgtattcg
    54721 gcgtagcccc cgtgatgggc cacgccgatg tcgaagctgg tggcgatcac cggatcgccg
    54781 gccttgaagc gcgaatccgc actgctcacc accgtgccgg acaggtcgat gccacccaca
    54841 cagggaaagc gccggatgat cttgccggca ccggtggccg ccagcgcatc cttgtaattg
    54901 acactggaat aggccacgcg gatcagcact tcgccggcgt cgagctgatc aacggtcata
    54961 cgctccatgc ggctgtccac cttgcgctgc gcgtcctggt ggatcacgaa agcctggaaa
    55021 tcggcgggaa gaggggtgtt catgcggttc tcctgatata gcgggcggct gccctggtta
    55081 tggttctgcg ccgattatat cgcccgcccg gcggctgttt ccggcccgcc gtgcgttgct
    55141 caatgtcgag cccctgctgc cgtaaatggg aacatcgccc cccaggagac cgtccatggc
    55201 cctgatcacc cgccctttcg aaaccctttc cgcttacgag gccttcttcg cccacgagga
    55261 gatgccggtg ctccggcaca cggtgaagga gctcgatgcc atgcgcgagc aggaggatca
    55321 tgtcaatggc cgcgccgtgg cggcgctggt cctgagcgat cccatgatga ctctgcgggt
    55381 attgatccac ctcgagcaga accggcgggc cagccagaac cacgacatca ccaccgtgga
    55441 gcgggccatc atgatgatgg gcatctcgcc cttcctgcgt cgctttgcgg acctgcccac
    55501 gatcgaggcg cagctgcacg atcatcccaa ggccctgatc ggtgtcctgc gggtcatcag
    55561 ccgggcccgc cgcgccgcca agtacgcccg cgactgggcc atcgtgcggc acgacctgga
    55621 tgtggacgaa atcaccgtcg ccgccctcct ccatgaggtc gcggagatcg tctgctggtg
    55681 cttcgccccg accctcaccg acgaggtcta cgccatgcag cgggccgacc acagcctgcg
    55741 ttcagtggct gcgcagcgcc gcgtcttcaa cgtctcggcc caggatctgc agtcctgcct
    55801 ggttcggcaa tggcaccttc cggaactgct ggtctcgatg atggacacgc accagatcaa
    55861 caacccccgg gtcgccacgg tcaagctcgc gggcaatctg gcccgtcaca ctgcccaggg
    55921 ctgggacaac gcggccatcc cggacgatct ggccgcgatc gaggcgctgc tgcatgtggg
    55981 ccgggaaacc ctgctgcatc gcctcgcggt gccccacgag gacgccgacc gcctgctcaa
    56041 tgccggcgga caggccgccg gagccctgcc gccggagcag tcgcctcagg cctgagccgc
    56101 ggcgcgggat tccagcgcct cccagcgcga cagggcggcg tcgatgtcct tctccagcgc
    56161 ttccagctgc gccttgagca caggcacctc ctgaggcgca tccttgtaga gcgtcggatc
    56221 acccagccgg gcctggacgt ccgcctgttg agtctcgagc gctgcaatgc ggtccggcaa
    56281 tccttccagt tcccgctgtt ccttccagct caatttcgcg ggccggtccg gtgctgcttc
    56341 cttacgcggc gggggtgccg ttttgggcgt cgcgggcaca actggggcga cggccggtac
    56401 ggcggccgga cgcgtacgca gccagtcctc gtacccgcct gcatattcct tccagtcgcc
    56461 ctccccttcc gcggcgatga cctgggtcac cacgttgtcg aggaaggccc ggtcgtggct
    56521 caccaggaag accgtgccgt cgtagcgggc cagcaggtct tccagcaggt ccagcgtctc
    56581 gatgtccagg tcgttggtcg gttcgtccag caccatcacg ttggccgggc gggcgaacag
    56641 gcgggccagc agcaggcggt tgcgctcgcc gccggacagg gatttgaccg gtgatcgggc
    56701 gcgggccggc gagaacagga agtcccccag atagccgatg acgtgggtgc gatgcccgcc
    56761 gatctccacg tagtccgaac ccggactgat gacctcggtc agcggcgcct ccgggtcgag
    56821 ctgggcgcgc agctggtcga aataggccac ggtctgacgg gtgccgcggc ggacgcggcc
    56881 ttcgtcgggc tcgatctccc ccaggatcag cttgagcagc gtcgtcttgc cggcgccatt
    56941 gggaccgatc aggccgatgc ggtcgccccg caggatgcgc gtggagaaat ggcgcgccac
    57001 ccagcgttca ccgaagcgtt tgcccacgtc ctccagctcg gccaccatct gcccgctgct
    57061 ttcgccacga tccaccgcca gcctgacgtt gcccaggcga tcacggcgct caccgcgctc
    57121 ccggcgcagg gcctccaggc gccgcacgcg cccctcgttg cgggtccggc gggcctcgac
    57181 gcccttgcgg atccacactt cttcctgggc cagcagcttg tcgaagcggg cgttggcctt
    57241 ctcctcggcg tcgagctgat cggccttgcg gacctggtag tcggcgtagc ggccgggaaa
    57301 actcgacagc agcccgcgat ccagttccac gatgcgcgtg gccacgtggt ccaggaagac
    57361 ccggtcgtga gtgatcacca ccacggcacc gggaaactcc cggatcagcg cttccagcca
    57421 caggatgccg tcgatgtcca ggtggttggt tggttcgtcc agcagcagca cttccggttc
    57481 gcccaccagc gcgcgggcca gagccacccg cttgatgccg ccgccggaga ggctgccaac
    57541 cggcgcgtca ccagcgagcc gcagcgtgcc gaggatctgc tcgacgcgtt gttccaggcg
    57601 ccaggcctgg gcgtcctcga cgcgatgctg gacggcctcc aggtgcgcca gcgcagcgtc
    57661 gctggcctcg tcggcgacgg ccagcatggc cgcgtggtaa tcgaccagca attgggccac
    57721 gtcaccgagg ccttcggcca ccgccgcgaa aacgctcagg tccagggaaa aatcggcttc
    57781 ctgcggcaca taggcgatgc gcacgccggg ctgccgccag acgcggccat cgtccagggt
    57841 ggcctgtccg gccagggcct tgagcaggct ggacttgccg ctgccgttgc ggccgatcag
    57901 ggcgacccgc tcgccggcgt cgagttgaaa gtcggccttg tcgagcaggg ccacgtggcc
    57961 gaaggccagg caggcatcgt ccagagtgag gagcggcatg aatcggtccg cgaacgcggt
    58021 cagggcagtg acgagaggcg gattctaccg cgggcacgcg atgcggcctg tccgggatac
    58081 gtcagtcgtg ttggcatgtt tgatgcgtca cacccctcga tacagcaggt gttgacggat
    58141 ttgcgacgaa tctatttaat agcaatcatt ctcattctcg aatcagcgac cgaggtgtgt
    58201 gcatgagtcc agccctgtcc ccgtcttccc agaccacgaa cgacgacgcg cgccgcgaat
    58261 ccgcatcgcc ggtcaccttg cgcagcgccg acctgctcca ggatcgggga tgcgtgctca
    58321 tcgaacacca aggcatggtc tacgccctgc gtgcgacccg cgccggaaag ctgatcctga
    58381 caaaatgatc ttcgtcgccc cttcgcgcca gccgggcgcc ttctccaccc ggcccatgtc
    58441 cctcctgggc gtcgccgcat tgctcgccac cgggcttgcc ggctccctgg gatccctctt
    58501 cctggtcctg catttctacg ccagctgaat cctggagcgc cctcgccatg tacgtctgtg
    58561 tctgtcgagc gatcaccgaa cgccatatca ccgaagccgt caatgacggc gcgcggcgcc
    58621 tgcgcgatct gcgccgtgat ctgggggtca cggaagagtg cggccgctgt gcccgctgcg
    58681 cccatgcctg cctcaaggag gccattgatc aaatcggtcc ggtctctata ctctcgggat
    58741 gtcggactga atcaacctat tccctatccc aggaggccgc atgaaaggcg acaagaaagt
    58801 gctgcagcac ctcaacaagc agctcaccat cgaactgacg gccatcaacc agtacttcct
    58861 gcatgcgcgc atgtacaaga actgggggct cggcaagctg ggcaagcatg agtacgaaga
    58921 atccatcgag gaaatgaagc atgcggacgc cctgatcgaa cgcattctgt tcctcgaagg
    58981 cctgcccaac cttcaaaacc tgaacaagct gctgatcggc gaaaacgtgc cggagtgtct
    59041 gcaaggggat ctgaagctcg aactgggcgg ccaggcagag ctcaaggaag ccatcgcata
    59101 ctgtgagacg gtgcgcgact atgtgagccg cgagctcttc gagtccatcc tcgaagacac
    59161 cgaggaacac atcgattacc tggaaaccca gattgagctc atcgacaagg tcggtctgca
    59221 gaattatctg caatcccaga tggacgtgcc gtcctgagcg agtcggtctc ggtagacgcc
    59281 agaaagcccg cgcaagcggg ctttttcatg aacacgtagc ggcgctgcgg cgcatgtccg
    59341 gcgtcggctg gcttcagggc gcgacgtgca ccggccgggt gagcgacttc tcctcgcagt
    59401 tcaggccggg tccttcccgg tcgatcacga gaaaatcgca gacttcatcc agggtgagca
    59461 gcgggtgatg ccacacacca ggggcaaaat tgacaccctg cccgtccgcg gccagaaaga
    59521 cccgcagtcg ggcctcgtcc ggcgtgtcgc ggccgtgcgc caccaccacc agatacgggc
    59581 gccccgacaa gggaacgaag gcctggctgc ccagcggatg acgttccagc atgctgacgg
    59641 gaaacggcat cggtcgcggc tggccccgga aaatcgagac gatggcccgc ccgtcggcac
    59701 cgggctcgat gcgggccaga tcgtggaagc gctccgccgt gccttcattg atcaggatcc
    59761 gctggcgtgc cgatgcagcc tcgatcacct cgccgaaggg cgcgaaggcg gccgacgtga
    59821 gcggttccgg acgcaggacg tccctttcgt cgcccatcat cgcgtcaccc gcccccacag
    59881 gcgcaggcgc gacacgccgc cgtccggaat gatattgaaa cgcacgtggg tgaccggccc
    59941 gagcccggcg atcccctccc ggaagacatg cagggcatcc atgcccaggg gctgctccgg
    60001 cagcagcacc ggccagaaca tgctctgggt gatgatggac tggtccgttc cgccatccat
    60061 acgggccgcc tggatcgagc agcggtccgg aaaattgccc ttgaagtggg cggtgtccac
    60121 ctcgatccgg tccacgacgc cgggggcgcc cagctgcagg atgcaccagt cgttgccggg
    60181 ttcccggcga cggcgcgtct cccaaccgtc gcccatgttg atgccgcgcc cgggcgcgat
    60241 cagattggca gcggaaccga agtgcgcgtc gttccaggcc accgcccgcc caccgttttc
    60301 gagggcgacg agatcgtatt cccgttcggg atccgacgtg tcgaatacgc ccaccggctg
    60361 gccgtacacc cgcagccggg caatgccgcc gtccggatag atgttgatgc gcagatggct
    60421 ccaggtatcg gaagactcga tctccagcag gtgatggctg ttgccggtca gggaagtgga
    60481 cggcaggatg cgcgtccagc gggcgcggac gagcaggtcc ggttcgtccg attcgcagtc
    60541 ggtggcgtcg atcgacactg ccggcgcgaa attaccggtg aaatggctgg tatcgatgtc
    60601 gaagccgcgc acgatgcccc gccgtcccag gcggacgagg ctccagtcgt aaccagtggt
    60661 gcgcttgcgg cgggtctccc agccgtccat ccacttcccg ttcgcgtcgt actgaccggg
    60721 cacaaagacc gcgggctccg gattgagcat gcgctccagc ggcgcgaaga attcgtcggt
    60781 ggcaaacagc gcccgcgcac cgatccggct gtcggccaga ttgacggagc ggcgcgccca
    60841 gtcgggcagg acggccggtg cggcgatgcg cggcggatcg aaggaatggg tcgtcatgca
    60901 ggcgctctcc aggggtgatg cgcaatccag tggcgggcaa tgtcaatgcg gcggcagatc
    60961 catacgcggt catgacgcgc caggtggtcg aggaatcgtt gcagggcgag gaatcgtccg
    61021 gggcggccca gcaggcggca gtgcatgccg atgctcagca tggccggacg ctcgtcgcct
    61081 tcggcataga ggacgtcgaa actgtcccgc agatactgga agaacgggtc gccgtggctg
    61141 aagccctggg gcagcgcaaa gcgcatgtcg ttggtgtcca gggtgtaggg cacgatcaga
    61201 tgcggcacac ggacgccatc gctgcgggtc acctgcaccc agaagggcag atcgtcaccg
    61261 tagtagtccg agtcgtagag caggcggccg tcgtcggcga cgaggcggcg ggtgttgggc
    61321 gagtcgcgcc cggtatacca gcccaggggg cgcgtgcccg tcatgtcctc gatgacgtcc
    61381 agggcctggg ccatgtgggc acgttcggtg gcttcatcca cattctggta atggatccag
    61441 cgccagccat ggcaggcgat ctcgtggccg agcgccacga aggcggcggt cagctccgga
    61501 tggcgctgca gggccatgcc gaccccgaag acagtcaggg gcagcccccg ccgctcgaat
    61561 tcccgcagga tgcgccacac gcctacccgc gatccgtatt cgtagatcga ctccatcgac
    61621 aggtggcggt ccggataggc ggccggattg aacatttccg aaaggaactg ctccgaaccg
    61681 ggatctccgt ggagcacgca gttctcgccg ccctcctcgt agttcagcac gaactgcacc
    61741 gccacacggg cgcgccccgg ccagtcggca tggggcggat gggcgccata tccgacgaga
    61801 tcgcgggggt attcggaatt gtgtatgggg ctcatgaggg cctcactcta tactgcgccc
    61861 cacatttttt cgtgaggatc gaaggatgga acgctcgccc aatagtccct accgggacga
    61921 catcgtgatc tcctggccgg aactgcaccg ggacacccgg ctgctgtgtc atcagttgat
    61981 ggaaaaaggc cccttcaagg gcatcatcgc catcgcccgc ggcggcctga ttccggcggc
    62041 cctggtcgca cgggaactgg agatccgcct gatcgacacg atctgcgccg ccagctacga
    62101 agccgacaac gacgacaccg cccagaccgt gcgccgggag gtgaagatcc tgaaaggcat
    62161 cgaacacgac ggcgacggct atctgctcat cgatgacctc gtggacaccg gcaatacggc
    62221 gcgggtcatc cgcgcccagc tgcccaaggc ctactttgcc acgctgtacg ccaagcccat
    62281 gggacgccag gtggtggacc tgtgtatcaa ggagttcagc caggacaaat ggatctattt
    62341 cccctgggac atcgactacc agttcgtgcc acccatcaag aagcatcatc gtcccgcctg
    62401 atcaacggta gccccccgcg gcccaggccg cgatccgttc gcgttcagag tcggtcatgc
    62461 ccgtgacgtt gcccagcggc atggccttaa gctgcacggc ctgagcgagg atccgcgggc
    62521 cattggcgcg gatcgcctcg gcgctgtcga gcagcacgcc agccggcgcc gccggaatca
    62581 gtgtcggccg ggccgcatgg cagccggtgc agcgcgccac cacgatgggc tggatgtcgg
    62641 ccagcgaggc cgccggactg ccggccgccg gcgccgcgtg ccggggcgcc agttcgacga
    62701 atacggccag cagcagcgcg gcacccagcg ccggatactg ccaggccacc tggcccttgt
    62761 ggcgcaggtt gaagaagtgg cggatcagca cggccgccag caggatcatc gccaggaccc
    62821 gccatgcctg gggatggttg tatgtgaagg cgtagtgatt ggagatcatg cagaacaaca
    62881 ccggcaaggt caggtagttg ttgtgcacgc tgcgctgttt ggcccgtttg ccgtggatcg
    62941 gatcgggcgt ctcgccccgc tgcatggcgg cgacggtggt cttctggccg ggaatgatcc
    63001 agaagaagac attgccgctc atggtggtcg cgatcatggc gcccacgtgg agaaaggccg
    63061 cccggccgga cagcagatgg gtgaggcccc aggccacggc caccacgaag gcgaaataca
    63121 gggcggcgaa cagcagcagg ctgcgattga tgagcagccg gcacagaccg tcatacacca
    63181 gccaaccgcc cgccagggtc gccagaccga tgccgatggc cgccgcagga ctcaatgccg
    63241 ccttggccgg atccaccatg taggttgccg cctgggagta atacagcaca ccaagcagga
    63301 agaatccggt gatccaggtg gaataggact cccatttgaa ccaatgcagt tcttcaggca
    63361 gatgctgtgg cgccacaagg tatttctgcg gattgtagaa gccgcctccg tggaccgccc
    63421 acaactcgcc accgacgccc ttgcggatca attctggatc ggtcggtgcc ttgagggaat
    63481 tgtccagcca cacaaaatag aatgaggagc cgatccacgc gatcccgaca atcaggtgaa
    63541 gccagcgcag caacagcgac cccacatcga tcagataagc ggcgtccatc agcttccccg
    63601 gtaggtggaa taggaccagg gcgacaccag cagcggcacg tggtaatgcc ccgccggatc
    63661 ggcaatgccg aacgccaggg gcacccggtc gaggaagggc ggctcgggca gtgcgacgcc
    63721 gcggttccgg aaatactcgg cgacggcaaa ggccagttcg tactgtcccg ggagcagggt
    63781 ctccccgctc aacaggggcg cttcactgcg gccgtcggaa ttcgtctgcc cggcagacag
    63841 cagcgtccgg caatcgccgt cgagacggta taactggaag tgcatgccgg cggccggaac
    63901 accggcggcc gtgtcgagga catgtgtggt caatcgcccc atgggacagc tccgcgtcgg
    63961 gaaggaggat gggtcgatta taaaaagccg cgcaggaagc gggacgtata aaggctatcg
    64021 gactttccag atgagcgacc ggacccgggg tgccgaaacg gtgatatatg aaagcgcgaa
    64081 agatctgtat tccgcctgcc ggcttgatgg ggtacgggcc ggcttcgtaa gcttcgggcg
    64141 tccgtcatat caaggggatc atcatgccct cgcgtttcac gcccgcccag ctgtccgacc
    64201 tcgatcgagc cgatttcatt gccgccatcg gtggtgtctt cgagcattca ccgtgggtca
    64261 tggcccgggc gtgggagatg cgtccctttt caagccgggc agacctggat gccgccctgg
    64321 tgaaggtcat tcgtgaagcc ggacgggatg aactgctcgc cctgatcgag gcccacccgg
    64381 aactcgccgg aaaggcagcg atccgtggcg acctgacggc cgattcggcg cgggaacagg
    64441 ctggcgccgg actcaacgcg tgttcgccgg aggagttcga gttgatccag cgcctcaatc
    64501 gggcctatcg ggcacgcttt ggctggccct tcatcgtcgc cgtgaagggc atgacgcgcc
    64561 acgacatcat cgcggccatt gagcgtcgcc tgccccagac ctccaccgag gagtttgtcg
    64621 aagccctggc gcagatcctc cggattgccg gctttcgcct ggacgaactg ctgaccgacg
    64681 cctgacgcgt cgggtcggcc ccacgggggg cagatccgcc cgccgccagc ctagaccgtg
    64741 gcaggcgttg cgacctgagc gggaacgcgc gtcccggcag cggcgggggc tccggtcttt
    64801 tcgcggatga cttcgatgat ctggtcacgg aaccagcggc attcctcgga atgatgggcg
    64861 cggtcgtgcc acaacagata aaagcgcatt ttcggaaaat cgaggggcga ctcggagaag
    64921 cacaactccc cgagggagca gcaatactcc gcaaagatgc gtggcgccga gaagatgatg
    64981 tcgttctgca gcaggacgta aggcaccaga ttgaaatacg gcacgctggc caccacgttg
    65041 cgcttcagac gctcccgcgc cagctgcatg tcgatgacgc cccgctgccc cacggcatag
    65101 ggcgtcggca ccaggtgttc ggcattcagg tagtcgtcga cggacagggt gcgcccggcc
    65161 aggggatgac tgcgccgcat caggcacacc acatcgtcgt cgaagagcgg cgccaggcgc
    65221 aggtgttcgg gaggttgggg ccagttgccg atgaccgtat ccagctcgcc ggattcaagc
    65281 gccttggcgt agtcgtagtc cggcccgaag gaatgcagga cgacctggga attgggtgcc
    65341 agccggcgca gacgcgccac gatgttgccg atgaggatcg cgttcaggta atcgggggtt
    65401 gcgatattga aggtccggcg ggacttcatc gggtcgaagc ggatctgctg gagtccgatg
    65461 gcctcgatct gggcgagggc gaccttgacc ggctccagca gcgccatgcc ccgctccgtg
    65521 ggggtcatgc cgttgcggct gcgcaccagc agctggtccc cggtgatgtc ccgcaaacgg
    65581 cgcagcgccg tgctgacggc gggctgcgac tgattgagac gccgcgccgc attggacacg
    65641 ctgcactcgg tcagcagtgc gtgcagcacc tgcaggagat gtacgtcgat ggattcccag
    65701 gagcaagtcg tggccatgat ttccctctga aacacgccgt atggtgatgt tgcagagcaa
    65761 aaatcgcgcc gatgggtgaa tcacgggaaa aagggcattt ccagccgaaa aacccacatt
    65821 gggcgcccga ttgcgcaccg ggagggcgtg ccggagggcc tgacggcacc atttcgatgc
    65881 ctgccacgca ccgaccggtc ggatcgtcac gctggatccc gtggcccatg aaaagacgcc
    65941 gccggcgtgg tgccggcggc gtcctgggag tgctgcgggc gaaggctcag aagtggtatt
    66001 cggcgcggag catcggcgtc ttggcaaagg caccgttgcc cgccgcgcca gtgtggtcgt
    66061 tgccaaactt gttgcgccag tactggtatt cgacgccgac cttgaaggtc ttcgggcgcg
    66121 caccgaccaa ggggctcgcg tcatacatga tctggccgtc gaagttggtt tccggcttcg
    66181 tcgggccgcc gaactcgttt gcgcccttgg cggcgatgta gttcatgaag ccttcaaagg
    66241 tcagcggcag ggagcccagg ggaatccccc aggcgagggt cagcatggga tgggcgtcgt
    66301 agctgtagcg gctcggatgg gtccgcggcg cattgctttc ccagagctgc agcacgctca
    66361 ggttgaggaa gccgggcaca tcgaacatca cggtcgggcc agccaggggc atgcgcttgc
    66421 gcgactggta gctgtcgccg gacttggtgt tgtaatcgaa accgacggtg aatcccaggc
    66481 cgcgcacgat gccgaacttg aaggcgtcgg gattgccggc gatcttgccg aaatcgaagg
    66541 tgtgacgata gaccacatag gtttcctggg ccccgccacc gttgttgaag gaatccttgc
    66601 tgtcggaaag gagcatgtcc acattgagga agttcgtgcc gtacttgtag ccgctcacat
    66661 gtccgagatt gaagatgttc ttcttgatgt cgtcaggatt gtacggttcg gcgaacttcg
    66721 tcccgtagcg ccagccgaca taggtgtcgc tccagtcggc agcgtgggca caggagaggc
    66781 cggcaatcag cattgcgcag cccagggcgg ttttcttgac cagcatggat ggacctccgg
    66841 aggtgttttc aagggggctc ccgcgctgtt tttgcgacgc agcaacgaga cgttggtcaa
    66901 actgtaacag gaagttttcc acgaaggccg aatacccgat atccggaaat gtgtatatcc
    66961 cggtcgttat acgtgcctga atataatggc cttattgtgt ccgatatacc cgtatagcat
    67021 gcatcaactt gaggcattcg aacggttttc gcaccgtttt gaggcgcgat ttgcggcgat
    67081 ttccggggtc acccccggtc acgcctcgcg cgatccgggt gagtcttttc ccgaaaatat
    67141 tttccactta taaaccaatg tgttaccacc tggcatgacg attgctgaag ctccgctcat
    67201 tcaaccgtcc aggagccctg tccatgccta tccccttcaa tgccggccgc cgtctggtgc
    67261 tggctgccgc catgtccctg ctggccatgc ccgcatttgc cgacgccccg acgcggatca
    67321 agttcaccct cgactggcgc tttgaaggac cggctgcgcc cttcctgttg gccaaggcca
    67381 aaggctattt cgcggccgag ggcgtggaca tggaaatcga ctccggatcc ggctcggcgg
    67441 gcgcggtcac gcgggtggcg accggcgcgt acgaaatggg cttcgccgac ttcaacgccc
    67501 tcgtcgaata cgatgccaag accccgggct ccaagatcca gggtgtttac atggtgtaca
    67561 acgccacgcc ggctgccgta ttcgtgctca agaagtcggg catcaccaag cctgccgatc
    67621 tggctggcaa gaccctcgct gcgccgatct tcgacgcggg ccgcaaggcc tggccggcct
    67681 ttgccaaatc caacaagctg gctgtggatg ccgtcaagtg gcagaccgtg gagccggccc
    67741 tgcgggaaac cctgctcgcc cgcggtgacg tggacggaat caccggcttc tacttcacca
    67801 gccttctcaa cctggaggcc cgcggcgtca agagcgacga catcgtggcg ctcaaatacc
    67861 cggacttcgg cgttgagctg tatggcaacg ccatcatcgc cagtcccaag ctgatggccg
    67921 agaatcccaa ggcggttgcc ggggtgctgc gcgccttcaa caaggcactc aaggaagtca
    67981 tcgccaaccc ggccgcggcg attgcctacg tcaaggaacg cgatccgctc atcaatgcgg
    68041 acctggaaac ccggcgcctc aagctggcgc tcgactccag tgtcgtcacg ccggaggtca
    68101 agctcaatgg actgggtgcc gtgacgcctg agcgcatgca acgctccatc gccgagaccg
    68161 ccgaagccta cgcgctgccc aaggtgccga cagccgccga tctgttcaat ccgaacttcc
    68221 tgccgtccaa gacggaccgc acgctcaagt aagccctgcg ccgcgggacg ggcgcacgtg
    68281 ccccgtccct cccctcctcc accggaactc cccatgtcgt tcgtcacctt tgaagacgtc
    68341 agcctggcct atacgccggg cggcgcaaat gccatcgaga acgtgagcct gagcatccgc
    68401 gagggcgagt tcgtcgccgt tgtcggccct tccggctgcg gcaaatccac cctcatgaag
    68461 ctggtgtcgg gcctcaagcc gcccagcgcc ggttacgtct atgtggatgg ccgggaagtc
    68521 ggcggccccc tgaagatcgt cggcatggcc ttccaggcag ccaacatgct gccctggcgc
    68581 accacgctgg acaacgtgat gctgcccctc gagatcgttg agccttaccg cagccgcctg
    68641 cgtcgcgagc gggagacctt caaggctgac gcggaagccc tgctggcacg ggtgggcctg
    68701 gccggtttcg gtcagaaata tccctgggaa ctctcgggcg gaatgcagca acgtgcctcc
    68761 atctgccggg cgctgatcca tcagccccgt ctcctgatgc tcgacgaacc cttcggcgcg
    68821 ctcgatgcct tcacccgcga agagctgtgg tgcatgttgc gtgatctgtg gcaacagcag
    68881 cccttcacgg tggtgctggt gacccacgat ctgcgggagg cggtgttcct tgcggacacc
    68941 gtctacgtga tgagcaaacg ccccggccgc atcctgatcc gcaaggagat cgatctgccc
    69001 cgcccgcgtc cgctggacgt cacctacacc gacgcctttg ccggctacgt gcacgagctg
    69061 cgcgagcata tcggccgcat tcgcgaatcc tgaggagcac ccccatgtcc ctgaagcaat
    69121 ccaccctcgt gaagagcgct ccctggctcc tgaccgccgt gctgttcatc gtctgggaac
    69181 tgatctgtgt cggctttgcg atccctgact acctgctgcc ggcccccacg gcgatctggg
    69241 cctccggcct ggagaacgcc gcccccatca tgatgcacgc cacccagacc ctgatgacca
    69301 ccctggccgg gttcgccctg gcggtggtcg tcggcatggt cctgggcctg gcggtcggcg
    69361 cctcgccggt ggtgtacaag gccacgtacc cgctgctggt cggattcaac agcatcccca
    69421 aggtggcctt cgtgcccgtg ctggtggtgt ggttcggcat cggcaccatc ccggcgatcc
    69481 tcacggcctt cctgatctcc ttcttcccgg tggtggtgaa cgtggccacg ggcctggcca
    69541 ccctggagcc ggagctggag gacgtgttgc gctccctcgg cgcccatcgc tgggacatca
    69601 tggtcaaggt gggactgccc cgctccctgc cctacttctt cgcgtccctg aaggtggcca
    69661 tcacgctcgc tttcgtcggt tccgtgatct ccgagaccgt cgcgtcgaat ctgggaatcg
    69721 gttacctgat gatgtccgcc agctcatcaa tgaacatgcc cctggtgttt gccgggctga
    69781 tcgtgatcgg tgtgatgggc gtggtgatgt acgagctgtt tgcgctgctt gagcggaacc
    69841 tcaccggctg ggcccatcgc acgcccggcg cagccacctg aaccctaagg cgccgcactg
    69901 ggtggaaggg tgaaagaggc tcgaaggggc ctcttgctcc accaccggac ggatgattca
    69961 tgcaccggtc cataccgacc ggccccctct cagcgctgga acgcaggcgc gccgagcacg
    70021 tgtgtggcca cgacacagcg ttcgtcgccg agcaccgtca gggcaaagag gcgctccgcc
    70081 agccccgtga caccgcgcag gcggaactcc agttccggcg tcgcgcgctc gtcgagcacc
    70141 acgcagtcgg cctccttgcc gacctcgaaa ttcccgatga aggcatccag gtacaaggct
    70201 cgggcacccg ccagagtggc gagataccag gcgcgcaggg gcgacagggg acgccggtgg
    70261 acctgggaga ccttgtaggc ctcgtcgagg gtacggatca ggctgaagga actgccgccg
    70321 cccacgtccg tcgccagccc gacccgcagc ccggcctcca ccgacgctgc gtggtcgaag
    70381 aaaccgctgc ccagaaacag gttcgacgtc gggcagaagg ccgccgccgt gccggtcgct
    70441 gccatctcgg cacgctccgc cccggacaga tggatgcagt ggccatagat cgtgcgcgga
    70501 ccgagcagac catggcgcgc atacacgtcg agatagtcgc ggcactcggg aaacaaggcc
    70561 ttgacccagg cgatctcgtc cggattctcg gccagatggg actgcacatg cagatcgggc
    70621 cggctcgcgt aaagttcgcc ggccagggcc agctgggccg atgtcgaggt gggcgcaaaa
    70681 cgcggggtga tcgtgtaacg caggcgcccg cgcccatgcc agcgctcgat cagggccaca
    70741 cagtcccgct cggcgtcggc aacggtgtcg cacaattcgg gcggtgcatg acggtccatc
    70801 atcaccttgc ccgtcagcat gcgcagttgg cgcgcgtccg cttcggccat gaaggcctcc
    70861 accgacgcgg catgcaccgt agcaaacacc gacgcggtcg tggtgccgtg cgccagcagg
    70921 cggtcgagga aataccgtgc cgtggcgcgc gccacctccg ggtcgcggaa ggccatctcg
    70981 gccgggaagg tataggtatt cagccagtcc aggagctgcc ggccaaagga ggcgatcacg
    71041 gccgactggg gatagtggca gtgcgtatcc accagtcccg gcaggaccag ccgatcgcca
    71101 tagtcgaaac gttcggcggc ggccaggcgt tccggcgcga tcgcagactc cacggcacgg
    71161 cggtgcccga cggcagccac gcggccctcc acgatccaca gggcgccctg gggaaaatgg
    71221 gcgtagctgt gggggtcgtc cccttcgccg ggatcgtcga ggaaatgcag gatctgcccg
    71281 aggatcaggc ggtccggaac ggtccgatgc gtcatggcgg cctctcaggc gcgatggtcg
    71341 gccagctggc gggccaggcg gttgtggtgg gcgatcagtg gcggcagttc caggggcacg
    71401 agctgacctt cccggaccac cggctggcca ttgatcaccg acaccttgac tttcggcggc
    71461 gtgcagaaga ccagggcagc caccggatcg tgaaccgcgg cgccggccag atcaagttcg
    71521 tcggtgggaa aagccaccac gtcgccgctc atgcccggcg ccagggcgcc gatatcgtcg
    71581 cgccccagca cccgcgcgcc gccgagggtg gcgagctcca gggcctgccg ggcgctcatg
    71641 gccgacggtc cgtagccgac ccgcgccagc agcatcgcct gacgggtctc gccgagcaga
    71701 tgggccgcgt cgttggacgc gcagccgtcg acgcccaggc ccaccggtac gcccgcggcg
    71761 cgcatcgccg gaatcggcgc gatgcccgag gcgaggcgca tgttggaaca ggggcaatgg
    71821 gccacgccgg tgccggtacg ggcaaagagt tcgatccccg gtccgtccag cttgacacag
    71881 tgggcatgcc acacatcggc gccgacccag ccgagggatt ccgcatattc ggcaggcgac
    71941 atgccgaatt tttcccggct gtaggccacg tcgttgtcgt tctcggccag gtgggtatgc
    72001 agcgagacgc cgtggccgcg ggccaggatg gcggcctcac gcatgagttc gcggcttacc
    72061 gagaatggcg aacagggcgc caccacgata cgccgcatgg cgtgccgtgc gggatcgtgc
    72121 caggtctcga tgagacgctg agtgtccttc aggatggccg cctcgtcctc caccagccgg
    72181 tctggcggca ggccgccggc gctttcgccg acactcatgc tgcctcgcgc ggcatggaag
    72241 cgcataccca ggatcgcggc agcctcgatg gcgtcgtcga ggcgactgcc gttgggatag
    72301 aggtacaggt ggtcgctgga ggtggtgcac ccggagagga tcagctccgc catggccgtg
    72361 agcgtggaca cccggatcat ctccggcgtg agacccgccc acaggggata cagccgccgc
    72421 agccagccga acagttctgc gtcctgggca ccgggaatgg cccgtgtcag gctctggtac
    72481 atgtggtggt gcgtattcac caggcccggc agcgctacgc acccggcaag ggagatcacc
    72541 tcgtcggccc cgtcgggaag ctggtcggac ggtccgaccg cctctaccac gccatcgcgg
    72601 atgaagacgc cgcccccggg aatttcccgg cgggtggcgt cctgggtgac gaggacttcg
    72661 gcgtccttca gcagcagggt ggccatcgcg gtcagggcag gaaggatttg ccgagggagg
    72721 ccggcaggtt gagctgaagc aggcgccggt cccgggagat gaacaccagc acgatcacgg
    72781 tcgccgcgta cggcaaggcg gcgaggagct gcaccggcac gtccagacca agcccctggg
    72841 cgtggaactg gaggatcgtc accccgccga acaggtaggc accgaggagc agacgcagcg
    72901 gcttccaggt ggcgaacacc accagcgcaa cggcgatcca gccccgcccg gcgctcatgt
    72961 tctgcaccca caggggcgta tagacggtgg acagataggc gcccgccaga cccgccatgg
    73021 cgccgccgaa gaccaccgcg ccataacgga tcgcgatcac cgggtagccc accgcgtggg
    73081 ccgcttccgg ggattcaccg acggccttga gaagcagtcc aagccgggtc ctggcgagga
    73141 tccaggccac gccggcgaag gccgcaaagg acaggtacac cactgcatcc tgatgaaaca
    73201 ggactggccc gagcaccgga atgtcggcga gcaacggaat gtccaccggc gcaaggcccg
    73261 gcaggctgta gctcacgtaa tgctgcccga tgaaggccga caggccgatg ccgaagatgg
    73321 tcaacgccag cccgcaggcc gcctggttgg cagccaggga caaggcaaac acggcgaaca
    73381 gcagcgccgt cgccatgccg gccagcgcac cggccggcaa gcccgccgcc gcaccgccac
    73441 cgctctccgc cgccgcggcg aagccagcga tcgccccggt gagcatcatg ccctcaaccc
    73501 ccaggttgat cacgccggtg cgctcggcca ccagttcgcc cagccccgca aagatcagcg
    73561 gcgtggcggc cgcgatggcg ccggccagta caggcacaag atgttcgatc atggcggatt
    73621 acctcgaagg acggcgcaga cggttgtcga tgaaggcatc ggcccccagc aggaaaaaca
    73681 ggagcatgcc ctggaagatg ccggtgatgg ccgccggcat ctgcagactc acctgggccg
    73741 cctcgccgcc caggtagatc agggccatca gggcgccggc gagcacgatc ccgagcggat
    73801 gaagtcgccc caggtaggca acgatgatgg cggcgtaccc gtagcccggc gagatgttga
    73861 ggttgagctg accgaccggg cccgccacct cacccacgcc cgccagcccg gccgtgaggc
    73921 ctgacatcgc caggctgaac cacaccgtct ggctggcgga aaagccggca tagcgggctg
    73981 ccgcgggcgc ctggccgccc acttccagct ggtaggccag gaagctgcgg gcattgaaca
    74041 gccagaacgc cagcaccgca acgagcgtga tgaacaccga gctgttgacc cgcgagcctt
    74101 ccagcaggat gccgaacagg gcgttgtcat cgaacatgat ggactggggg aagttcatcc
    74161 cctccgggtc ccgccagggg ccgttcacca tgtaagacag gatgaaggtc gccacgtagg
    74221 tgagcatcag cgtggtcagg gtttcttccg cgttgaagcg ggtcttcagc agcgccggca
    74281 acgcgcccca cagggccccg ccgatggcgc ccgccaccac catggccgga atcacccacg
    74341 cggaggtgct gccgttgaag tggatcgcga cccccccgcc aaacagcgcc ccgacgatca
    74401 gctggccctc ggctccgatg ttgtagatgc gcgccttgaa gccgatggcg agcccctggg
    74461 cgatgaggat cagcggacag gccttgatca gcagctcgct ccatccgtag ctgctggtca
    74521 ggggctggac gaagaagacc tcgaaggatg tgagcggtga tttgtccagg gcccagaaga
    74581 gcagcgaacc ggtcagcagg gtggcagcga tggcgatgag gggcgccgtc cagcgcatga
    74641 gctgggacgg ttgggcacgg ggttcaagca gatgcatgga cggattctcc ggattgggcg
    74701 gcggtgaaca gtccggacat ctgcagtccg accttctccg cgttggtcgc ggccttgggc
    74761 tccggttcgg cgagcttgcc ggccgccagc accgcgatgc gatcgctgat ctcgaagagc
    74821 tcctccagct cttccgacac caccaggatg gccacgccgg tgcgtgacag gtccagcagg
    74881 gtctggcgca ggaaggccgc ggcccccacg tccacgcccc aggtgggctg ggcgacgatc
    74941 atcaccttcg gcgacagcat gatctcgcgc ccgacgatga acttctggag attaccgccg
    75001 gacagggagc gggccgccga ggcggcgcca ccgcaacgca cgtcgaagcg ggcgatgcac
    75061 tgctcggcga aggcgcgggc cttggcgtaa ctgaccaggc ccaggcgctt catgccctgc
    75121 cggtgggccg tgagcagcgc gttgcgggcc agcgacaggg acggcaccgc accccgtccg
    75181 aggcgctctt ccggaacaaa gacgagcccc cgatcccgcc ggcgacccgc gtcgagatgc
    75241 ccgatcgccg caccctccaa gcaaatcgcg tcgcgcgcca ccggcgtctc gccggacagg
    75301 gctgccagca actcggcctg tccgttgccg gagatgccgg cgatcccgag gatctcgcct
    75361 gcgtggacgg tcaggctgat gtccttcaaa gacgtgccga acggatcgtc cgacggcagg
    75421 ctgagaccgc ttacctgcag caccgggcgg ctttcgccga tgaagggcgg cgcctcgcag
    75481 accggcagct cgcgaccgat catcatgcgc gccaggctgg cgggcgtctc gtcccgcggc
    75541 gtcgcgttgc ccgtcacctt gccgccgcgc atgatcgtcg cggaatcgca caaggccttg
    75601 atctcgtgga gcttgtggct gatatagaga atcgagacgc cttccgccgc cagcgtgcgc
    75661 agggtgtcga agagcttgag cacggcctgg ggcgtgagga ccgaggtcgg ctcgtcgagg
    75721 atcagcagct tgggatcctg cagcaggcag cggacgatct ccacgcgctg gcgttcgccc
    75781 accgacaggc tatgcaccag ccggaagggg tcgaggggca ggccgtagcg ctccgacacc
    75841 tcgatcacgc gcttcgccag cgccggcaga tcgaaggctt cgtcgaggac cagggcgatg
    75901 ttctccacca cggtcagggt ctcgaacagg gagaagtgct gaaacaccat gccgatgccc
    75961 agcttgcggg cctgcgccgg gttggcgatg gccacctggt gcccgttcca catcacctcg
    76021 ccttcgtcgg ccttgaccgc accgtagatg atcttcatca gggtgctctt gccggcaccg
    76081 ttctcaccca ggatggcgtg gatctcaccg ggcattacgg tgaggctgat gtcggaattg
    76141 gccaccacgc tgggatagcg cttggtgatg tggagcagtt gaagtcgggg ttcgggcgtg
    76201 ctcatgctta ctccggctgg acggggttgt cgatcaggcg ggggcgggcc gcgtcgccca
    76261 caagtcggcg ggcttcgtga acctggaaaa tttcggccgc gacggccagg gcaatggcgg
    76321 caggcgcctt gtcgacgatg ccggcgatcc cgatcgggca gaccagttca tcgaggcgtt
    76381 ccggattcag gcctcgatcc accaggcggt gctcgaagga tctacgcttg ctacgggagc
    76441 cgatcagccc gaaataggcg aaatcccgcc gccggaagat ggtctcgcac aggctgaaat
    76501 cgagggcgtg gctgtgggtc atcaccacga aaaagctgcc ggcgggtgct ctcgccacct
    76561 cgctttccgg cacgtcggtc agcaccgtgc ggacatccgt cacgtccaga ccgtcgaagg
    76621 cgccgtcacg ggtgtcgacc caggtgatct gcgccccgag cagctgcagc tggcgcacca
    76681 gcgcctgggc cacatgaccc gcaccgaaaa gctggatcgg gaacgccaga ccgccgacat
    76741 gctgggcgaa tcgccagtct gccgccgtgc ccccgagccg cgtctcgcca cctgccacgg
    76801 gcgcccgctg ccagaccgaa gcggcggcct ccgaatccac ctggcgatgc aggtgatggc
    76861 ctgccagcac cgacgcgcgg cgctccaccc acgcatcccg ctcggacgcc ggcatcgact
    76921 cgaacaccag ccacaccacc ccgccgcagc actgacccaa acgtgcgccc aggttgtagc
    76981 ggacgatttc cggacggccg ccgcccacgg cgaggcgccg ccgggcatga ctgatggcct
    77041 cccattccag atgccccccg ccgatcgtgt ccacgatcgt ctcgcgaccc accagcatgg
    77101 ccgcccccgc ctcgcggggg gtcgatccat ccgcccgggc aaccgtcacg agttccacgc
    77161 tctgccccgc ttccaggaca gcgggcaagg ccttcagcca gtcgggcgcg cgggtcgtca
    77221 tgcgccatcc tccggcgcca ggcagcccag cgcccgcagg acggcttccg gcgtggccgg
    77281 cgcctccagc cggggtgatt gccccgccgg cagggcggcc gccacggcat cgcgcagggc
    77341 catgaagacc gacagggcca gcatgaaggg cggttcgccg acagccttgg agcggtggat
    77401 gctgtcttcc acgttggcat tgtcgaagag ccgcacgttg aacacgtccg gtagatcggt
    77461 ggcagtcggg atcttgtagg tggacggcgc gtggctcatg agccgcccgg cctgggggcc
    77521 gtccggtcgc caccacagtt cttcgctggt gagccagccc atgccctgga tgaaaccgcc
    77581 ttcaatctgg ccgatgtcca gggcaggatt gatggagcgg cctgcgtcgt gcaggacatc
    77641 cacccgcagg acgcggcatt cccccgtcag ggtatcgatg gccacttcgc tcgccgcggc
    77701 gccgtaggca aagtaataaa acggccgccc cagcttggtc tgcgggtcga agtggatctt
    77761 cggcgtacgg tagaagcccg catcccacaa ctggatgcgg gcccggtagg cgcgcatgac
    77821 cagtgccgcg aagctgtcgt cgaacagcgg tgcggaggcc tcacccgcct gcacgcgcgc
    77881 atcgacaaag cgcacctgct ccgctgccac ggccccctga acgagttcga cgacgaaggc
    77941 cgcgaggcgt gccttgatgt gctggcaggc ggcctgggcg gccttgccat tgagatccgt
    78001 gccgctcgat gctgccgtgg cggaggtgtt ggcgacccgg ctcgtgtcgg tggacgacag
    78061 acgcacatcc tccagcggca gcccgaactc gtgagcgacg atctggcgca ccttggtgta
    78121 caggccctgc cccatctccg taccgccgtg ggagacgagc accgtgccgt cggtatagac
    78181 atgcaccagc gcacccgcct ggttgtaatg ggtggcgttg aaggagatgc cgaacttgac
    78241 cggcgtcagg gcgagaccgc gcttgatgac tggactgctg gcattgaagg cgagaatcgc
    78301 cgcgcggcgg gccccatagt cggacgcatc ggccagttgc gcgaccagcg ctggcgccac
    78361 gttgtcctcc acggtcatgc catagggcgt tgtgctgcgg gcgccgggca cgacgtcgta
    78421 gaagttcgcc tcccgcaccg ccagggcgtc caggcccaga tgccgggcaa tgtcatcgag
    78481 gaccgtctcg atgacgaaca tgccctgggg gccgccgaag ccgcggaatg ccgtattgga
    78541 gacccggttg gtcttgcagc gcaggctgcg cagggccacc gcatccagat aataggcgtt
    78601 gtcaatgtgg cagatcgtac ggtcgttgac cggccccgac agatcggcgg aatacccgca
    78661 gtcggaggcc atctcgagcc tgagcccgag gatccggccg gcgtcgtcga aagccgactc
    78721 ccagcggtat tgatagggat ggcgcttgcc cgtggcgacc atgtcgtcgt cccggtccag
    78781 gcgcagcttg accgggtgac ccgtcttcac cgcgagcagg gccgccaggc aggcccactg
    78841 ggccgactgg gattccttgc cgccaaagcc accgcccatc cgccggcatt cgacgctgac
    78901 ctggtgcgag cgccagccca gggcgtgggc caccatctgc tgcatctcgg tgggatgctg
    78961 cgtggagcag aacacatgga tggtgtcgct ctcccgcggc acggcacagg aaatctggcc
    79021 ttccagataa aagtgttcct gcccaccgag gcgcgcagca ccggacagcc gatgcggggc
    79081 gttttcgaag gcctgttcga ccgcgccgcg cgtcatcacg aacggcggca gcacccagga
    79141 ttcggccgcg atggcgctgt ccatgtcgag aatggggggc tccgcggcgt actccacccg
    79201 cgccagacgc gccgcctggc gcgccgcctc gcgggtctcg gccgccacca cgaagatcac
    79261 ctgcccgtaa aactcgacaa ggtcgccagt gatgatcgga tcgtcgtgca ggatcggtcc
    79321 gcagttgttc tcgcccggga tgtcggcggc cgtcagcacg cagcgcacgc cgggatacgc
    79381 ccgcacggcg ctcagatcca tggcacggat gcggccgcgg gcacaggtgg acaggccggg
    79441 ggccgcgtgc agggtgcccg ccatttccgg caggtcatcg atgtactgcg ccttgccgct
    79501 gacgtgcagc ggggcgcttt catggggaac cgagcgaccg accggggtcg cgccggaggg
    79561 agacatccgc gcgttcatga gcgttcctcc ggctggatcg ggcccagcgc agacagccgc
    79621 gcaggcagac cgtcgggcgg cgccgtcgtc tccagccaga agcgctgcaa cagattcgcc
    79681 gcgaccgaca ggcgataggc ggcgctcgcg cgcacgtccg tgaggggcgc gaagtcttcg
    79741 cgcaaggcgg ccatcgccag ccgaaccgta tccggcgtcc acgggcgccc cagcagggcg
    79801 gcttcaccgg catcggcccg cttgggcgtg ccagccacac cgccgaaggc cagacgcgcg
    79861 ccccgcacga tgtgctcgtc gtccagatgc acggcaaagc cgcaggccac tgcggagatg
    79921 tcctgatcga agcgcttcgc caccttgtac gccctgaact gccggttcag gggtggcagc
    79981 ggcgaatcca gcaccagcgc acggatgaat tccccgcgct ggagagcggt cttctgataa
    80041 tcgagataga acgcttcgag gggcatctcc cgcgtgtgcg cgccctgttg cagcaccacc
    80101 cggcagccga gggcgatcag cgcgggcatg gagtcgccga tgggcgaacc attggccacg
    80161 ttgccaccca gcgtgccggc attcttgacc ggcgtggagg cgaaacgccg ggccagttca
    80221 tcccaatgcg gctcaaaggc ggtgagcgcc gcgaaggcgt cggtaaggga cacaccggcg
    80281 ccgatctcga gctgcccggc accgacccgg atgcgcttga gctccgccac gccccccaac
    80341 tggatcacgt ctcccagatc ccggccctgc ttggtcaccc acaggcccac gtcggttccg
    80401 ccggccacca gtcgggcgtc cggcagacgc tcgcgcagct cggcgagctc ggtcagtgtc
    80461 cgtggcccgt agctgtgccg cccggccgcc gcataatcga gcggcggcaa ttcggccagc
    80521 gacttcaggg ccgcgacgat aggcgcccgc tccaggctcc gacgcggcgc cagaaaggcg
    80581 tcgcgggccg catccacgat cggccgatag ccggtgcacc ggcatagatt ccccgacagg
    80641 cagtggtcga tctcggcccg cggcggacag atcggggtgt tttcgtacag tgcgtacagg
    80701 gacatgacga aacccggcgt gcagaacccg cactgggacc catgccgctc cacgagggcc
    80761 tgctggaccg ggtgcaggtg cccgtctgcg ctcagatcct cgaccgtgaa caaggccttc
    80821 ccgtccaggg ccggcaggaa ctggatgcag gcattgacgg cacgcaggcg cagttcgtcg
    80881 ccttccggcc ccttcgccag ttcgccgatc accaccgtgc acgcgccgca atcgccttcg
    80941 gcacatccct ccttggtgcc ggtctggtgc agatcttcgc gcaggaactg gagcacggtc
    81001 cgcgtcggtg ccggtccctg aacggactgc acggcgccgc ggaaatagaa ctggatcggg
    81061 cgtatgggca tggcgggctc acaacggatg atgcggtgcg acaaacctta gcacaggctt
    81121 ttccgggtcg ttgagagggg acgacggata agggttatgt gccgaagcgt atggctgctg
    81181 gcgggaccta ttcgcgcgtc cagaagcccg cgtggggcgc cgcagcctcg tcttccaggc
    81241 agggtccggt gacggcgata cggcgctgtc cccgggcaaa cacctcgcgg catggcagcg
    81301 agaacgtggg attctccggg tgatcgccgg tgaggccgag caggcgatgt tccgacaggg
    81361 cgtacaccac ccgtccgata ccgcaccagt agatggcgcc ggcacacatg gcgcagggct
    81421 cggcgctggt atagagggtc gcccggctca gttgcccggg cgtcaggcgg cgcgccgcaa
    81481 gcccggccgc gaccagttcc gcatgccgcg tcggatcgcc atcgggcggc acggaatcgt
    81541 taccggcctc ggcaacgatc cgcccgagtt catccacgac gatggcggca aacggatgct
    81601 tgcccccatc acgcacgcgg cgcgacagtt cgatgcagtg gcgcaggaag gtttcgtcgg
    81661 cggctgtcag gatgtcggac atggcggtct ccggaaatgg tgacgattat cccggtacgc
    81721 accgggccga catgtcagtg acgggcaata ctcttcataa cgccggcgag ataggtgcgt
    81781 cgggcatgga ttggtagagt ggcgcaacat cccgttttct gaccacctta ggagatccaa
    81841 gcatgttgct gcgcagactc aagtccttca cagcggttgc ggtcatggcg ctggcgggcc
    81901 tgcacgcggc ccacgcggcc gaaccgatca aagccggctt cgtctatatc ggccccaccg
    81961 gcgatcacgg ctggacctac tctcacgatg aaggccggaa gctgctggaa gcccagtcca
    82021 acggcagcgt gaagaccacc ttcgtcgaga acgtgcccga aaccgccgac gccgaacggg
    82081 tcttccggga tctggcgcaa aagggcaaca aggtcgtctt cgggacctcc ttcggttaca
    82141 tgaaccagat ggccaaggtc gccaaggcct tcccgaacac catcttcatg cacgccaccg
    82201 gatacaagac cgcccccaat ctgggtgtct acgacgtgcg gacctacgaa ggcgcctaca
    82261 tgctgggcgt ggtggccggc aagatgagca agggcaacaa gctcggcgtc gtcgcctcca
    82321 ttcccatccc cgaagtcatc cgcaacatca atgccttcac cctcggcgcc cgcagcgtca
    82381 acccggccat caccacgcgg gcgatctggg tgaacagctg gttcgacccg ggcaaggagc
    82441 gggaagccgc gctggccctg atcgcccagg gctgtgacgt gctgatgcag aacaccgact
    82501 cgcctgccgt cgtccaggcc gcccaggaaa agggcgtgct ggcctttggc tgggattcgg
    82561 acatgaccaa gttcggcggc aaggcccatc tggccgcgtc cgtgctgaac tggggcgtga
    82621 tctacaagaa gaccgtcgat gaagtcgctg ccggcacctg gaagagcggt gacatctggt
    82681 ggggcgtgaa ggaaggcgcg gtgaatatcg acaacttcgg cccggcggtc cctgcaccgg
    82741 tcaagaagct tgccgaagaa cggcgcgatg ccatcaaggc cggcaagctt caccccttca
    82801 ccggcccgct caaggagcag tccggcaagg agttccttgc cgccggcaag atctttggcg
    82861 acggagatct gaagaagatg aatttctacg tggaaggcgt cgaaggctcc atcccgaagt
    82921 aagcagcacc ccggcttgaa cgcgacctgc gggtcgcgtt tttcattgct gcgacgcatt
    82981 gccgatcgac cggtttatgc tcggcgtacg accatccctg aagaggtaaa catgccccct
    83041 cccctcgctg ccgatgccgc gctcgacttc gcctgccgca tgcccaaggc cgagctgcac
    83101 gtccatatcg aaggcacgct ggagcccgaa ctggcctttg ccctggcccg tcgaaacggc
    83161 atcgccctgc cctatgcgga cgaggacgcc ctgcgcaagg cctacgcatt cgacagcctg
    83221 cagtcctttc tcgatctgta ctacgcgtgc gcggatgtcc tgcgcacgcc cgacgatttc
    83281 cgcgatctga tgctggccta cctggagcgg gccgttgccg acaacatcgt ctacaccgag
    83341 atttttttcg atccgcagac ccatacggcc cgtggcattc ccttcgaaac cgtcctgagg
    83401 ggtctcgaag acgggctgta tcacgcccgc cgcctgtggg gcatcgaagg gcgcctgatc
    83461 ctgtgtttcc tgcgtcacct gagcgaggag gacgcatttg ccaccctggc agaagccgag
    83521 cctttcctgg agcgcattca cgggttcgga ctcgattcgt ccgagcgcgg tcatcctcca
    83581 gccaaattcg cccgcgtctt cgcccgctgt cgcgcgctgg gcaagccgat cgtcgcgcac
    83641 gcaggtgagg aaggcccgcc cgcctacatc agcgaagcac tcgacctgct gggggcccga
    83701 cgcatcgatc acggcgtccg cgccgtcgag gatgccgccg tcgtcgaccg cctgatccgg
    83761 gacgcgatcc cgctcacggt ctgtcccctc tccaacctgc gcctgtgcgt gtttccgagc
    83821 atgcaggacc acaccctggg ccgcctcgtc gccgccggtg cctgcgtcac cctcaatagc
    83881 gacgatccgg cgtatttcgg aggctacctc aacgacaaca tccgcgccgt gcacgcggcc
    83941 ttcgcgttcg atatacccac ctggtatcaa ctggcccgca acagcttcca ggccagcttt
    84001 gcaagtccga ccgaaaagca gcagtggatc gaccgcctgg atgccgcccg cgcagccact
    84061 ggtctctgat tcctcgccat gcaggcgcag gaacaaatcg cccgacccgt cgcgccttgg
    84121 cagatgaatc cgatctgcgc tgacgtaagc gactgcggca ggaagcccaa gggcagttat
    84181 tgagaacggc cacccgccag cccacccagt aagtgccttg agaacgtcct cgtgcccgaa
    84241 cacccgtgaa gtcacgacga acgtcgcctc gcatgcaaaa agcccgtgaa aacgggcttt
    84301 ttagcgtact gcatatacca gtgaaacagc ccggcaggtc gcctggtccc cccgacagga
    84361 atcgaacctg tatctagcgc ttaggaggcg ctcgttctat ccattgaact acagggagtc
    84421 gaaggggcgc attctagcgt gttgcgggca cttcacgcca cgttggcgtg gcgcagaccg
    84481 gggcgcggac cgtcagtgca atcaggcaaa gacgtccaca ttccgcccga ccgtcgcagt
    84541 cggatccgga gcctttgctg gctgagggag cgcctggagc agttgctggg cgttggcctg
    84601 ttgctgatcc agggtcttgc gcagcatcgc gatggagact gggtcggccc catttgcagc
    84661 ggacgtggag ggggtgggtg cgatgttcat gatgtcagtc gcggaagtta ccgtattgca
    84721 aagggtagtc cgtgatgtcc ttgcgcacca gggcaataca ttcttgcagg acgtcccgct
    84781 tggcgccgct cacgcgtacg gcatcgccct ggatgctggc ctgcaccttg agcttgctgt
    84841 ccttgaggag cttgacgatc ttcttggact gctcctgctc gataccgacg cggaccttca
    84901 gctcctgctt gaccttgttg ccgcccacct tctgcacgtc accgtggtcg aggcagcgca
    84961 catcgatctt cttggcggtc atctcgggga gcaggatggc cagcatctgt tcgagctgaa
    85021 aatcgctgtc gccgtgcagg gtgagcagct tttcggtctg ctcgacccgc gcatcgctgc
    85081 ccttgaagtc gtaacggccc gtgatcttgc ggttggcgcc gtccacggcg ttgcgcaggg
    85141 cgaccatatc gacttcggag gtgatgtcga aagacggcat ggtcggctcc cgctcagccg
    85201 aaatggcaga cgtagtggta gggctcgccg ctgacttcga tgtcgaaggc ggagttggcg
    85261 ggtacattga aggattcgcc cgcgccatag gtcttccact cggtttcacc cttcaggcgc
    85321 acgcggcagg cgccagcgac gccttccatg atctcgggcg caccggtgtt gaaggtgagc
    85381 gtggcgggca ggatcacgcc gacggacttc ttggtgccat cggcgagcac gatggagtgg
    85441 ctgacgcact tgccgtcgaa atagacattg gccttcttga cgacggagac ggtatcgaac
    85501 tggacgttct gggtcatggt gagcgttcct gaaaatttat tcgatgccca ggagccgctg
    85561 gatcagcccc ttggcgatga aaccgatgaa tccgacgccc agaccgacaa acatccagat
    85621 cgcgccgtac ttgccggctt tcgattccca ggcaagctgg ccgatgatga agaccatgta
    85681 ggcgatcagc ccgccgacgc agagcttgag ggagatgtcc tcgaactcgg caacggtgag
    85741 gccgaagaac aatggggcgc tcggatccat catgagcgtg ctcagccccg cttggcccgc
    85801 gcgttggcgg cgatgcgcat gcgcagggcg ttcaggcgga tgaagccgtg ggcatccttc
    85861 tggttgtagg cgccctggtc gtcctcgaag gtggcgatgg tcggatcgaa cagggaatcg
    85921 gtcttggagt cccgtgcgac aacaatcacg ttgcccttgt agagcttgag gcggacccag
    85981 ccattcaccg tctgctgggt ctggtcgatc agggcctgca gggccagccg ctcgggcgac
    86041 caccagtagc cggtatagat catgctcgcg taacggggca tcaggtcgtc cttgaggtgg
    86101 gcgacttccc ggtcaagggt gatggactcg atggcgcggt gggcgcgcag caggatggtg
    86161 ccgcccgggg tttcgtaaca gccacgggac ttcatgccca cgtagcggtt ctccaccaga
    86221 tcgaggcggc cgatgccatg cttgccgccc agctcgttga gcctggcgag cagttcatgg
    86281 ggcttcatgc gggtgccgtt gagggcgacc agatcgccct tctcgaattc cagatccagg
    86341 tattcggccg catccggcgc cgcttcaggc gacacggtcc agcgccacat ggattcctcg
    86401 gcctcggcgg cggggtcttc caggtgacgg ccttcgaagg agatgtgcag cagattggcg
    86461 tccatggaat agggggagcc gccctgcttg tgcttcatct cgatgggaat gccattcttc
    86521 tccgcatagg ccagaagctt ttcgcgggac agcagatccc actcgcgcca cggcgcaatg
    86581 accttcacgt tgggcatcag cgcgtagtag cccagctcaa aacgcacctg gtcgttgccc
    86641 ttgccggtgg cgccgtggga caccgcatcg gcgccagtgg cgcgcgcgat ctcgatctga
    86701 cgcttggcga tcagcggacg ggcgatggac gtacccagca ggtattcgcc ctcatagatc
    86761 gtgttgcagc ggaacatggg gaacacgaag tcccgcacga actcttcccg caggtcgtca
    86821 atgaagatgt tctccggctt gatgccgaac tgcagggcct tggcacgggc tggttcgagt
    86881 tcctcgccct gtcccaggtc ggcggtgaag gtcaccacct cgcattgata cgtgtcctgc
    86941 agccatttga ggatgaccga ggtatccagc ccgccggaat aggcgagcac caccttcttt
    87001 acgtcgctca tgtcgtggtt ccgctcttgt tacacatcac aattcgtttg acacccgggc
    87061 agtccggccg ccactcagct ttcgaccttc ccgatcagca gatattccat cagggccttc
    87121 tgcacgtgga gccggttctc ggcttcatcc cagaccaccg actgcgggcc gtctatcacc
    87181 tcggcggtga cttcctcgcc ccggtgagcc ggcaggcaat gcatgaacac tgcacccgga
    87241 ttggcgaccc gcatcatgtc ttcatctacg cgccactcgg caaagtcgcg catgcgctct
    87301 tcgttctctg cctcgaagcc catgctggtc cacacgtcgg tggtgaccag atcggccccg
    87361 cggcaggctt ccatcggatc gtcaaagtgg gcaaagtggc ccgtgccgat cagcccggcc
    87421 cgctccggct ccacctcgta acccggaggc gtggagacgt gcacatggaa atccagcacc
    87481 tcggccgcct gcagccacgt gttgcagaca ttgttggaat ctcccaccca ggccaccgtc
    87541 ttgccctgga tcggaccgca gtgctcgatg aaggtgaaga tgtcggccag gatctggcag
    87601 ggatggtatt cgttggtcag cccgttgatc acggggacgc gggaatactc ggcaaagcgc
    87661 tcgatgatcg actgctcgaa ggtgcggatc atcaccacat cgctcatgcg tgagatgacc
    87721 tgggccgcat cctcgacggg ctcgccacgg cccagctggg agtcgcgggt attcaggtag
    87781 atggccgagc cgcccagctg ctgcatgccc gcctcgaagg acagacgcgt gcgcgtactg
    87841 gccttttcga agatcatcac cagcgtccgg tcgaacagcg ggtgataggg ttcgtaacgc
    87901 ttgaacttgt ccttgatcca gcgcgtgcgc gcgaacaggt aatcgtattc ggcccgggag
    87961 aaatcccgga actggagata atgtctcggt gctttcatgg gcactcgttt tctaggttat
    88021 tgaccagcaa gaaattcctt taccagcggc gccagcccct ccaccagcgc cgcggcctcc
    88081 tcggcgctga aattcagcgg cgggagcaga cggatgacct tgtcagccgt cacgttgatc
    88141 agaagaccgg cctgcagggc gcgccccacc agttcggcgc agggacgatc gagctcgatg
    88201 ccgatcatga gacccgcgcc acgaatatcg atcacgccgg gcagcccggc cagcgccacg
    88261 gcaaaccctt cccgcagggc ctggcccatg cgtcctgcgt tgtccagcag gcgctcgtcg
    88321 tccagcacgg ccagggtttc cagtgccgcg gcacacgcca gcggattgcc gccaaaggtg
    88381 gagccgtggt tgcccggttt gaagacgccc gccgcccgtc ccgaagccag gcaggcgccg
    88441 atcggcacgc ccgagcccag tcccttggcg agcgacatca cgtccggctt gacgccggcg
    88501 ttctgatggc caaaccactt gccggtccgg ccgacgccgc attgcacctc gtcgaacatc
    88561 aacagccatc cgttcgcgtc gcacatgcca cgcagggctt ccatatatgc cgagtgagcc
    88621 acgtgaatgc cgccctcgcc ctgcaccggc tccaacagga tcgccacgat gtccgaacga
    88681 ccggcagtcg cctgctccag cgctccgaga tcgtcgaaag gaacccttac gaatccaccg
    88741 accagcggct cgaagccctt ctggaccttg gcgttgccgg tagccgacag ggttgccagc
    88801 gtgcgtccgt gaaaggaatg ctccatcacc acgatggccg gctcggcaat gcctttgccg
    88861 tgcccgtaca atcgggccag cttgatggcc gcttcattgg cctcgcagcc ggaattgcag
    88921 aagaacactt catccatgcc cgccagcgcc gcgagccggt cggaaagctg ttcctggcgc
    88981 ggaatggcgt acagattgga ggtgtggatc acctgtgcag cctgttcggc aatggcgcgc
    89041 accaggcggg gatgattgtg cccgagtgtg gagacagcaa tgcccgagag ggcatcaagg
    89101 taacgcttgc cctcggtatc aaacagccag gatccctgtc catggctgaa agccaccggc
    89161 aggcggccgt aggtattcat caggtgcggc atggaaaccc ttgtttcata atctgccccc
    89221 ccgcacccga gctgtgaagc ggggagccgg aaaaggcgaa acggcgactc gcgccgccgt
    89281 ttaaatgcaa tatcgcatcc taattcaaaa ccttagcctt gtatagcggg cgcgtgtgtc
    89341 ggcgccccgg cgcgacgcgg ccttccgtaa cgcgaaatgg cgtctttcct gatagaatcc
    89401 agcttccgca cggatgcagt ccagtcgccg cagagcctcg gattgcgagc cgcctttccg
    89461 acaccctccg cctgcaccca tgattcaaaa acttgaacat ttcatgattg tcggccgcac
    89521 ctccgacggc aagactttcc gtccgagcga ctgggcggac cggttgtgcg gaatcatgtc
    89581 ggccttcggc gccgacaacc gcatgaccta ctcgccctat gtccgtccgg cgtgcaatct
    89641 ccggggcgac aagacggtat tggtggacgc gcgcattcac gacatcgagc cgcttgccta
    89701 caacttcctg aagaatttcg ccaaagacaa cgaccttcag gtcgaagtgc tgccggacga
    89761 tcacttcgac tgagacgcgt gggcgcgacg gtgcctgctc cccgcacatc gcgacagcgc
    89821 aaaaaaagcc ggctcgaaag ccggcttttt catttagcga acgcagcagt cagtgcttga
    89881 cgacgccgcc gttgtcggcc ggttgatccg cagccgcagc gaccgggact tccggcgccg
    89941 gcgtgtcggg caacggttcg ggccgacgtt ccagcgccag ttcgagcacc tcgtcaatcc
    90001 acttgacggg gatgatctcc agcgcattct tgacgttgtc cggaatctcg gtgagatcct
    90061 tgacgttctc ggacggaatc aaagcccgcg tgatgccacc gcgcacggcc gccagcagct
    90121 tttccttcag gccgccgatg ggcagcacct caccccgcag ggtgatctcg cccgtcatcg
    90181 ccacgtcaca ccgcaccggg atgcccgtca gcactgaaac caggccggtg cagatggcga
    90241 tgcccgccga cggaccgtcc ttgggaatcg cgccctccgg caagtgaatg tggatgtcgg
    90301 agttctggta gaaatccgcc gcaatgccca gggacttgga gcgacgccgc accaccgaca
    90361 gggccgcctg gatggactcc tgcatgacct cgccgagctt gccggtggtc atgatcttgc
    90421 ccttgcccgg cagcgccacg gcctcgacgg tgagcaattc accacccacc tcggtccatg
    90481 cgagcccggt gacctgtccg atctggttct gcttttccgc aacgccgaag gtgtacttgc
    90541 gcacgcccag atacttgtcg atgttcttgg cgttgaccac caccttggcc ttgcgcttgc
    90601 cgagcacgat cgccttgacg accttgcggc agaccttgga gatctcacgc tccagcccgc
    90661 gcacaccagc ctcgcgggtg taaaagcgga cgatctcgcg gatcgcgtct tccgtcaccg
    90721 agagctcttc gcgcttgatg ccgttgttct tcatctgctt cggcacgaga tagcgctggg
    90781 cgatgttgac cttttcgtcc tcggtgtagc cggacagacg gatcacctcc atccggtcga
    90841 gcagcggcgc cgggatattg agggtgttgg ccgtcgccac gaacatcacg tcggagaggt
    90901 cgtaatccac ctcgatgtag tggtctacaa aggtcgcgtt ctgttccggg tcgagcactt
    90961 cgagcagcgc cgaggacgga tcaccgcgga agtcctgccc catcttgtcc acttcgtcga
    91021 gcaggaacag ggggttgcgc acgcccactt tggtcatgtt ctgcaggatc ttgcccggca
    91081 tggagccgat gtaggtccgg cggtgaccgc ggatctcggc ttcgtcccgc acgccgccca
    91141 ggctcatgcg cacgaacttg cgattcgtcg cgcgggccac ggactggccg agggaggtct
    91201 tgcctacacc cggagggcca accaggcaca ggatcggcgc cttgagcttg tcgacgcgtt
    91261 gctggacggc aaggtattca aggatgcgtt ccttgacctt ctcgaggccg taatgatcct
    91321 gatcgagcac cttctcggcc tcggccagat ccttgctgat acgcgacttc ttcttccacg
    91381 gcagacccac cagagtctcg atgtagttgc gcaccacggt ggcttcggcg gacatgggcg
    91441 acatcaggcg cagcttcttc agctcaccct gggccttggc agcggcttcc ttggacatgc
    91501 cggacgcctt gatcttcttg tccatctctt ccagatcggc gccgtcctcc ccttcgccga
    91561 gttctttctg gatggccttg acctgttcgt tcaggtagta ctcgcgctgg ctcttctcca
    91621 tctggcgctt gacgcggccg cggatgcgct tttccacctg aaggatgtcg agttcggtct
    91681 cgagctggct gagcagacgc tccaggcgtt cgcccggatt gaacatctcg aggatttcct
    91741 gcttctgctc cagcttgagg ggcaggtgcg cggcaatggt gtcggccagc cgacccggct
    91801 cttcgatgcc cgacagggag ctgaggatct ccggcgggat cttcttgttg agcttcacgt
    91861 actggtcgaa ctggctgatg atcgcccggc gcatggcctc gacctcgttg ttcgg
//
LOCUS       DAOQMM010000003        90476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_31_length_90476_cov_49.791343, whole genome shotgun sequence.
ACCESSION   DAOQMM010000003 DAOQMM010000000
VERSION     DAOQMM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 90476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 90476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90476
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31_length_90476_cov_49.791343"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLH85_00925"
     CDS             <1..530
                     /locus_tag="PLH85_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63150.1"
                     /translation="LRIDWPAGLRAAERAAFTAHRFHSVFGDMGTWAILADQGAVGFP
                     SLACAIPAALVAAIDEAAQSRTLRVASLQPAYVSDFNRCRGRFRDDGAFARLETARIT
                     VGLWGDGRWRAFRSHPVELADAAAAGRCLTALLKTLPLEDLVPAGTLYTRGGDAVGPD
                     ALPAGWRGEAVEDAP"
     gene            644..1087
                     /locus_tag="PLH85_00930"
     CDS             644..1087
                     /locus_tag="PLH85_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63151.1"
                     /translation="MLVLREHGQLDERVAGERARLAQFRHGVAKTASVPSPPTAPLSE
                     GEIRPAVAVARVLQRDWLALFGALETAMADPGVALLSVEQDAARGPLKLAGEARNLPE
                     VFALIHRLESGGVLRDVRLANYDFRSSGSVQVVGFVLGAHWEGGP"
     gene            1165..1632
                     /locus_tag="PLH85_00935"
     CDS             1165..1632
                     /locus_tag="PLH85_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63152.1"
                     /translation="MLLLALAADGLVNRTLAAEAEALRQEARVVVSLRVRPLSPEESD
                     RQRLDRFYASFPDSAGLPDRLIRINGYALARQVAVDRAEYRSSKEPGTPLERVTLNVP
                     AHGDYPTLRAWLGDVLTDMPELAVESLSLKRSTIADPVLEAQVHLAIFLKSVP"
     gene            1671..2114
                     /locus_tag="PLH85_00940"
     CDS             1671..2114
                     /locus_tag="PLH85_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63153.1"
                     /translation="MLLVAWVAGLEDDDLPAPPSVRRVPPRPTARSVGSTALDVPPRR
                     SVAPDMRNSSGDLFPVVSFEPPPPPPPPSLKPVAPPLPFRYAGGLDERGDMTAFLADG
                     EQIRVVRAGELIDGRYRVVLISRVRIDFLYLPLNEPQSLATGNLP"
     gene            2111..4390
                     /locus_tag="PLH85_00945"
     CDS             2111..4390
                     /locus_tag="PLH85_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspD"
                     /protein_id="HNB63154.1"
                     /translation="MNCSIIRRVLALVAALLLVVGCAANPALEESRKAFATGHPEAAL
                     RSLQDKVSADPKNLELRAYFLRQRDTLILRQVLAADQARIAGRFDEAERALAVARPFD
                     PRNPRVIAAAEAIGAQKQRKLQMEDAERRFSKGDLAGAEAAVRAVLAADPSHPVASAL
                     MRRLDERAAALDSAPLALAGPFSKLVSLEFRDAPLRGVFEALSRASGLNFVMDRDVRP
                     DIKVTLFVRDNSIDDVIKLLMVTQQLERKLLNGNSVLIYPNTPVKQKDYQELVTRSFF
                     LANADPKQAMAMIKQMVKTRDVFVEEKLNLLVMKDTPAAVRLAERLVADLDLAEPEVM
                     LDVEVLEVSRNTLLELGTRFPDQIGYGQLQPTTTSSVTTPTGTTLTQSLGGQLLQGNI
                     NLRQTGELVPFVANPGLLLNLKDQDGNSNILANPRIRVKNRDKAKIHIGEKLPVFTTT
                     STANVGVSSSVNYLDVGLKLEVEPSVHLDDEVAIKVNLEVSSIAKEVTGPAGSLAYQV
                     GTRSAITSLRLRDGETQVLAGLISDEERSSANRLPGLGELPGVGRLFSSQRDVGNKTE
                     IVLLITPRILRNIAKSGSASPTQAAGTESTVGALPLLIGATRAGALGLKSPAGAALPP
                     AVAQALVASAAAPTGVSASVEGVRPALRLEVPDEVHPGEEFAVTVTLESVAGEGCSLQ
                     MGFDSSLVDIVDGQSERGDVVVLQLPAGPASSLAVRFRARPGAVGRAGFSLLSAQGLT
                     PGQGQAVLLPAPGGVQIVP"
     gene            4394..4864
                     /locus_tag="PLH85_00950"
     CDS             4394..4864
                     /locus_tag="PLH85_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB63155.1"
                     /translation="MSGRGFTLIEMLVTVAVVAVLAAMAVPVGLVTVQRAKEGELRTA
                     LRQIREGIDTYKRYVDEGRIARAADANGYPPHLEDLVRGAPDAKSPGAARIYVMRRIP
                     RDPFNTDAAIAASETWGRRSYASPPDAPEEGRDVFDVYSRSPSIGLNGIPYRDW"
     gene            4864..5250
                     /locus_tag="PLH85_00955"
     CDS             4864..5250
                     /locus_tag="PLH85_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB63156.1"
                     /translation="MTPRRPIPCKGFTLVELLVVMAIVAMLLSIAAPRYFGHLARARE
                     AALAETLAVTRDAIDKFNGDVGRYPRDLDELVTGRYLRALPLDPLTERRDDWILVAPP
                     DGGEGVSDLHSSAQGTARDGRAFNAL"
     gene            5247..5780
                     /locus_tag="PLH85_00960"
     CDS             5247..5780
                     /locus_tag="PLH85_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB63157.1"
                     /translation="MMRRKHQGGATYLLTLFAVAALGVALARFGAVWTTTLQREREAE
                     LIFIGGEFARAMASYRSAGSGTEPEAPPELESLLLDPRFPYVRRHLRKLYRDPMTGKA
                     DWLIERQGGRIVGVRSRSTQHALRCAALPAWVRNVARVPEDARYSDWLFHPEERSGAR
                     NHGETASESGSLWPPSR"
     gene            complement(5901..5976)
                     /locus_tag="PLH85_00965"
     tRNA            complement(5901..5976)
                     /locus_tag="PLH85_00965"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5941..5943),aa:Asn,seq:gtt)
     gene            complement(6026..6101)
                     /locus_tag="PLH85_00970"
     tRNA            complement(6026..6101)
                     /locus_tag="PLH85_00970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6066..6068),aa:Asn,seq:gtt)
     gene            complement(6198..7406)
                     /locus_tag="PLH85_00975"
     CDS             complement(6198..7406)
                     /locus_tag="PLH85_00975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNB63158.1"
                     /translation="MSSSIFAAVDMAPRDPILGLNEAFNADTRNTKVNLGVGVYFDDN
                     GKIPLLAAVKTAEKARLEAAPPRGYQPIEGPAAYNNAVQNLLFGAASPLLSDGRVVTA
                     QALGGTGALKIGADLLKRVVPGATVYISDPSWENHRALFEAAGFPVENYPYYDPATRG
                     ANAEGMIATLNSLPAGSIIVLHACCHNPTGADLTEAQWAEVVKACATRGLVPFLDMAY
                     QGFADGIEPDAVAVNLFAASGLSFFVSSSFSKSFSLYGERVGALSIVTQNKDESARVL
                     SQLKRVIRTNYSNPPIHGGAIVAAVLSTPELRAQWEAELAGMRERIRAMRTGLVEQLA
                     ARKVDQDFSFVIKQRGMFSYTGLSAAQVERMKTEFGIYAVGTGRICLAALNSKNLDYV
                     ADAIAAVLKA"
     gene            7525..9603
                     /gene="uvrB"
                     /locus_tag="PLH85_00980"
     CDS             7525..9603
                     /gene="uvrB"
                     /locus_tag="PLH85_00980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNB63159.1"
                     /translation="MADQTDSGRVVCFEGSPFRLHQPFPPAGDQPEAIRLLAEGIGDG
                     LMFQTLLGVTGSGKTYTMANVIARCGRPALVLAHNKTLAAQLYAEFREFFPENAVEYF
                     VSYYDYYQPEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLMERRDVVIVASVSCIYG
                     IGDPVDYHAMVLHLREGERMAHRDLVQRLVAMQYTRSDVDFRRGTFRVRGDVIDIFPA
                     ENAEYALRVEMFDDEIEHLTLFDPLTGHLKQKIVRFTVYPSSHYVTPRATVLRAIEAI
                     KDELRTRIQTFQTGGKLVEAQRIEQRTRFDLEMLNELGFCKGIENYSRHLSGRAAGEP
                     PPTLIDYLPADGLMFIDESHVTIGQVGAMYKGDRSRKENLVEYGFRLPSALDNRPLKF
                     EEFERLLPQTVFISATPAAYEAAHQGQVVEQVVRPTGLVDPVIHVRPASTQVDDLLGE
                     IKLRVAVEERVLVTVLTKRLAEDLTEYLAENGIRVRYLHSDIDTVERVEIIRDLRLGH
                     FDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHLHGTAILYA
                     DRITDSMRAAMGETERRRARQLAFNAENGITPRSVTKRIKDIIDGVYDAKDEDAAVVA
                     AEPAAEYAAMDEKALARTIRQLEKDMLEHARNLDFEKAAAVRDQLFRLRRQAFGAETH
                     DTSLPPDANR"
     gene            9684..10178
                     /locus_tag="PLH85_00985"
     CDS             9684..10178
                     /locus_tag="PLH85_00985"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNB63160.1"
                     /translation="MMKILFVCTGNICRSPTAEGVARRWLAMTGLEGIVGVDSAGIQG
                     YHAGEAPDPRAQTAAMRRGYDLSRLRARKVLLKDFAEFDLILAMDRDHFHYLRDMAPE
                     GCKHKVRMFMSFAQTHRRDDVPDPYYGGITGFDAVLDMCEDAVQGLIDDIRPDVERRH
                     RSVV"
     gene            complement(10286..13153)
                     /locus_tag="PLH85_00990"
     CDS             complement(10286..13153)
                     /locus_tag="PLH85_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNB63161.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEQRKSNIYKA
                     VITRIEPSLEAAFVDYGAERHGFLPFKEISRAYFQPGVEASRASIKEALKEGQELIVQ
                     VEKDERGNKGAALTTFVSLAGRYLVLMPNNPRGGGVSRRVEGDERAELRDVMDQLEVP
                     GGMSLIARTAAIGRSAEELQWDLNYLLQLWRAIDGAAQAQAGAFLIYQEGSLVIRAIR
                     DYFQPDIGEILIDTDDVYEQARQFMAHVMPTNVNRVKRYHDDVPLFSRFQIEHQIESA
                     YSRTVNLPSGGAIVIDHTEALVSVDVNSGRATRGADIEETAFKTNCEAADEVARQLRL
                     RDLGGLIVIDFIDMESQKNQREVENRLRDALRYDRARVQTGKISRFGLLELSRQRLRP
                     ALAETSYIPCPRCNGTGHIRSTESSALHILRILEEEAMKDNTGALHCQVPVDVATFLL
                     NEKRDDIAKIEVRHKVNLILIPNRHLETPQHEIVRLRHDQLNQEEHVLPSYKMATQPS
                     DVTYTPPSASNDRPARQEAAVKGITPDQPAPTVEAKPAPAPAPVPESQPGLLARIFAF
                     FRGEKQPEPAPVVAQPEPARRESRSRNDRNRRDGRNRRDGQREERGERAERSERSERN
                     EPRDTRAPEAAAETREKPQRQRGERQERGERGERPERAERGERSERGEGRGRNRNSAQ
                     APRAVETEAAVSDADNKLEAVASVAVVDAAMPAPTSEGEGNGERNRRRRGRRGGRRNE
                     ESGTSESAAQGELEIGTAAAEVAEAVPAAEADTPAVTDTPAIEPAVVEAVVPQQVETV
                     SAAAASVEPVFEPAAEVASDAAPVTAEAPVVDAVAPVAAVEETVPQETPTEPETAPVP
                     EPGVADAPAAAPSFFATPVEVPLEAPAAAPVVEALPVVEEAVATLDETLKDSGLILIE
                     TRRDAAAPVAPVEPAQPLGRRRRAVAPVAEEPLVQVETGAK"
     gene            13641..14561
                     /locus_tag="PLH85_00995"
     CDS             13641..14561
                     /locus_tag="PLH85_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB63162.1"
                     /translation="MVDESAAGQRIDNFLIRHCKGVPKSHVYRILRSGEVRVNSRRVA
                     QTYRLQSGDEVRIPPLRLAEREDSSPVPVGKAFAIAYEDEALLVLDKPAGLAVHGGSG
                     VSFGVIEQLRRQRPEARFLELVHRLDRETSGLLIVAKKRSALTVLHDMMRDGRVQKRY
                     LTLVAGRWMNPLQHVKAPLLKYLTEGGERRVSVHPDGKPAHSIVRLHRRWQSCSLVEV
                     ELKTGRTHQIRVHLTHQGFPLYGDDKYGDFALNKQLEKAGLKRMFLHAAHLAFRHPLT
                     DAPIELSAPLPDELAAFIVAVEQAEERDYG"
     gene            14554..15210
                     /locus_tag="PLH85_01000"
     CDS             14554..15210
                     /locus_tag="PLH85_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNB63163.1"
                     /translation="MVKRFDLIVFDWDGTLMDSAAAIVRAIQSASTDLGLPVPDDARA
                     RHVIGLGLVDALRHAVPDLAEHDYPRMVERYRHHYLSGDHELTLFDGTFDMVEALHGR
                     GHLLAVATGKSRKGLERALGFSGLGRFFHATRCADECFSKPHPQMLEELLDELGVMPD
                     ACLMIGDTTHDLQMARNAGVAALAVSFGAHPVEQLEAEAPLACLHSPVALRDWLERHA
                     "
     gene            15207..15545
                     /locus_tag="PLH85_01005"
     CDS             15207..15545
                     /locus_tag="PLH85_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNB63164.1"
                     /translation="MICRSDEVLEGGDGIRFTVQVCGEAVAAFLVRYGGRVYAYRNSC
                     AHVPVELDWQPGRFFDTGGTYLICATHGALYEPESGYCVAGPCRGRRLVPVAVEERDG
                     GVFLKGVLGA"
     gene            complement(15550..16260)
                     /locus_tag="PLH85_01010"
     CDS             complement(15550..16260)
                     /locus_tag="PLH85_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB63165.1"
                     /translation="MAGTLYLVPVSLGPAPTALTTPAGVAAQACALDYFVVENAKSAR
                     AELKRLEHPRPLRELDIRELPDKPNPADLDALLAPILAGTDGGLMSEAGCPAVADPGA
                     LLVRRAHEAGVRVVPLVGPSSLLLALMASGLNGQSFAFHGYLPVDEDARNRRIRELEA
                     ESGRQQRTQLFIETPYRNERMFEALRTVCAPTTRLCVARDLSLDSEWIATRTVAAWRK
                     SAAPELARHPTVFLILAG"
     gene            complement(16260..16835)
                     /locus_tag="PLH85_01015"
     CDS             complement(16260..16835)
                     /locus_tag="PLH85_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNB63166.1"
                     /translation="MKLVLASTSAYRRELLLRFGLPFDVARPDIDESPLPDESPRATA
                     ERLAVGKARAVAAQFPDALIIGSDQVAALGEHRFGKPGCVERAVAQLKTMSGQTVVFH
                     TALALLNTRNGAVQVDVVPTEVRFRALSEDEIVRYVERERPLDCAGSAKSEGLGITLL
                     DALTGDDPNALVGLPLIALARMLRAEGIALP"
     gene            16953..17429
                     /locus_tag="PLH85_01020"
     CDS             16953..17429
                     /locus_tag="PLH85_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNB63167.1"
                     /translation="MIDLRALARDGRHVEGEIPVARLERLADSVSDPVGSVWFRFDGV
                     RDDDGKLFADLMIRTELTLQCQRCLDALRLPCVIHNRLLLLRPGEEIPEDDLENDEVD
                     ALEVEPRTDVLALVEDELLLALPLVPRHEACEAPVKAGGDEEISPFAVLRNLRGKA"
     gene            17451..17630
                     /gene="rpmF"
                     /locus_tag="PLH85_01025"
     CDS             17451..17630
                     /gene="rpmF"
                     /locus_tag="PLH85_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNB63168.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLVAPPLAVEPTTGEVHLRHHVSP
                     SGVYRGKKVAKAKGE"
     gene            17709..18722
                     /gene="plsX"
                     /locus_tag="PLH85_01030"
     CDS             17709..18722
                     /gene="plsX"
                     /locus_tag="PLH85_01030"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNB63169.1"
                     /translation="MTVTLAIDCMGGDHGPSVTVPAALRFLDTDPDVRVVLVGRAEAV
                     EQQLGVAQVRYGERLSIRHATEVVGMDEPPALAMRNKKDSSMRVAVDLVKSGDAQAAV
                     SAGNTGALMAISRFVLKTLPGIDRPAICSTLPTLRGQTHVLDLGANVDCSPAHLLQFG
                     IMGAMLVAAVEHNERPSVGLLNIGEEDIKGNETVKQAGELLRHSGLNFYGNVEGNDIY
                     KGTVDVVVCDGFVGNVALKTSEGLAQMLATFLREQFSRNWVTKLAAVFAMSALKGFKH
                     KVDSRRYNGAALLGLRGVVVKSHGAADVFAFEQAIRRAVEAARNNLIERISARMERER
                     PAA"
     gene            18719..19684
                     /locus_tag="PLH85_01035"
     CDS             18719..19684
                     /locus_tag="PLH85_01035"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNB63170.1"
                     /translation="MIYARVAGTGSYLPGAPVTNDDLVARGIDTSDAWIQERTGIRSR
                     HLASPEVTSSQLAQEASLRALEAAACTPNDIDLVIVATSTPDYIFPSTATLLQARLGM
                     SNGAPAFDVQAVCAGFAFAIATAEKFIRSGSHKRALVVGAEVFSRILDWNDRGTCVLF
                     GDGAGAVVLEASERPGILATALHADGAQNQILCVPGQISRGQVTGDPFLRMDGQAVFK
                     FAVKVLSDVAKEVAELGGVPLDAVDWLIPHQANIRIIQATGKRLGVAPERVIATVGEH
                     GNTSAASIPLALDAAVRDGRIRRGQKVILEGIGGGFAWGALLLEY"
     gene            19717..20652
                     /gene="fabD"
                     /locus_tag="PLH85_01040"
     CDS             19717..20652
                     /gene="fabD"
                     /locus_tag="PLH85_01040"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNB63171.1"
                     /translation="MNFAFVFPGQGSQSVGMMAAYGDSAVIRDTFAEASAALGEDLWQ
                     MVADGPAETLALTVNTQPLMLTAGIAVYREWIARGGRTPALVAGHSLGEYSALVAAGA
                     LGFQDAVKLVRLRAQAMQEAVPAGEGAMAALMGLDQPAAAEACAEAAQGEVVSPANLN
                     SPGQIVIAGARAAVERAIAVAKTKGAKRGVLLPVSAPFHCALMKPAAERLAVALADIV
                     VAAPAIPVLNNVDVAVETDPARIKDALVRQAYSPVRWIESVQAIAGRGLTHVYECGPG
                     KVLAGMTKRIEGSLTGGAIADAASLDEILQTTGAA"
     gene            20649..21398
                     /gene="fabG"
                     /locus_tag="PLH85_01045"
     CDS             20649..21398
                     /gene="fabG"
                     /locus_tag="PLH85_01045"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNB63172.1"
                     /translation="MSQVLQGQVALVTGASRGIGRAIALELGRLGATVIGTATSEAGA
                     ADIQAGLDAAGVSGRGMMLNVTDAAACEAALAEIEKAFGPVLVLVNNAGITRDNLSMR
                     MKDTEWDAVIDTNLKAVFRMSRLVTKGMMKARNGRIINITSVVGSSGNPGQANYAAAK
                     AGVAGMTRSLAQELGSRNITVNCVAPGFIDTDMTKELPEDQKKALQTKIALGRLGKPE
                     EIAAVVGFLASPAAAYVTGATLHVNGGMYMA"
     gene            21486..21725
                     /gene="acpP"
                     /locus_tag="PLH85_01050"
     CDS             21486..21725
                     /gene="acpP"
                     /locus_tag="PLH85_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB63173.1"
                     /translation="MSDIEQRVKKIVAEQLGVNEAEVKNESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKITTVQQAIDYVSAHLNK"
     gene            21748..22983
                     /gene="fabF"
                     /locus_tag="PLH85_01055"
     CDS             21748..22983
                     /gene="fabF"
                     /locus_tag="PLH85_01055"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNB63174.1"
                     /translation="MAARRVVITGLGVISPVGNTVSEAWDSILNGRSGIGPVTRFDAS
                     ALSARIAGEVKGFDVGAYISAKEARRMDTFIHFGLAAGIQAIRDSGIEVTDANADRIG
                     VNIGSGIGGLPMIEGTHTDYVAGGPRKISPFFIPGTIINMISGNLSIMFGLKGPNLAV
                     VTACTTGLHAIGSSMRMIQYGDADVMVCGGAESTVCPLAMGGFASAKALSTRNDDPAT
                     ASRPWDKDRDGFVLGEGAGVMVLEEYEHAVKRGARIYAELAGFGMSGDAFHMTAPDTD
                     GPRRSMLNALRDAGVNPDQVQYLNAHGTSTPLGDKNETEAIKQAFGVEVAKTLVVNST
                     KSMTGHLLGGAGGLESVLTALAVHHQVSPPTINIFEQDPECDLDYCANVARPMKIDVA
                     LKNNFGFGGTNGSLVFRRV"
     gene            23014..23448
                     /locus_tag="PLH85_01060"
     CDS             23014..23448
                     /locus_tag="PLH85_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HNB63175.1"
                     /translation="MINVLEFGPSRLRVILLVTLHAAAAFSCVAGLASPWITAPVGLV
                     IAASLYREVKQALGQSGRLELHADGRLAWQFSGMAAQAGRVRAESVALHRSIWLIWDD
                     DGRRGARWLLPDQMSTQDWRALQVWLRLRVTKTFAEPDSDVA"
     gene            complement(23471..25054)
                     /gene="nadB"
                     /locus_tag="PLH85_01065"
     CDS             complement(23471..25054)
                     /gene="nadB"
                     /locus_tag="PLH85_01065"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNB63176.1"
                     /translation="MSVFDVLILGSGLAGQSLALRLADTHKVALVTKRHLEDSASAWA
                     QGGIAAVLDPADSIESHLTDTFVAGAGLCNPGSSRFVVENGARAIQWLIERGVPFTAD
                     AQSPIGYHLTREGGHGQRRVIHVADATGAAVQATLTEQVRAHPNIAIFEQHITVDLIV
                     GSKIGRLEEGCLGAYVLDIAHDEVRTFAAGHTVLATGGAGKVYLYTTNPDTATGDGIA
                     MAWRAGCRVANMEFIQFHPTCLYHPQAKSFLISEAVRGEGGLLRRPDGSRFMPEHDTR
                     EELAPRDVVARAIDFEMKKHGLDCVFLDISHKPAEFIAEHFPNIHARCLELGIDITQQ
                     PIPVVPAAHYTCGGVVTDLHARTDVPGLYAIGETACTGLHGANRLASNSLLECLVFAE
                     AAAQDIRSHLRHAPADLPTWDESRVTDADEAIVISHNWDELRRFMWDYVGIVRTTKRL
                     QRAQHRIRLLAREIDEFYSNFRVTNDLIELRNLVVTADLIVRSALRRKESRGLHFSRD
                     YPQLQPKARETVLRPPRRS"
     gene            25257..25856
                     /gene="rpoE"
                     /locus_tag="PLH85_01070"
     CDS             25257..25856
                     /gene="rpoE"
                     /locus_tag="PLH85_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNB63177.1"
                     /translation="MSDREIDQQLVERVQRGDKQAFGLLVAKYQRKLARLLSRLIRDP
                     AEVEDVAQETFIKAYRALPSFRGESAFYTWLYRIGINTAKNFLVSQGRRAPTTTEFDS
                     EEAETFEDGDQLRDVDTPERLLMTKQIGETVNLAMEALPEELRAAIVLREIEGLSYEE
                     IATIMGCPIGTVRSRIFRAREAISEKLKPLLDTAPNKRW"
     gene            25861..26406
                     /locus_tag="PLH85_01075"
     CDS             25861..26406
                     /locus_tag="PLH85_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNB63178.1"
                     /translation="MKERVSALLDGALDEESTVRMLDHLKRDGALRRDWEHFCLVGDA
                     LRGDEPLALDVTRSVMLRLEDEPTVLAPAVRREGSGFFRHLMPLAASLMGVAAVGWVA
                     GMFSGGGDALHFAKVNKSPALMASVTPATSGQSMVMPTSVENSNREYLFAHQSLAPSA
                     AMPGVALYVRTVADTAMPGQR"
     gene            26403..27371
                     /locus_tag="PLH85_01080"
     CDS             26403..27371
                     /locus_tag="PLH85_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNB63179.1"
                     /translation="MSRFRHCLLVLLACCGTARAEAPGDPLSWLGRIAGAAHRLNYSG
                     TFTYQHGKTVETSRIAHLVDGSGEFERLEALDGSPREVVRSNDQVQCYLPDQRMLIVD
                     QPSVRRSFPARLPPSVNTLAENYRIRKGEMVRIAGLESQQIILEPKDNLRYGHQFWAD
                     VNSGLLLRARLIDEKGDSIEQFAFSEVQIGVPLDRDKVRAHIVRDPDWKVVNARGTEV
                     RMEDAGWVFRNALPGYRQVVSVLRQIRQDRPEAYHVVFSDGLSAISVFIEPHAGKSSV
                     IQSGMSATGSFNILRRSAGDYLITVLGEVPAAALVRLADGIELRKK"
     gene            27371..27814
                     /locus_tag="PLH85_01085"
     CDS             27371..27814
                     /locus_tag="PLH85_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNB63180.1"
                     /translation="MRVPGVVQKVEQGFAWVEVTPAGGCGRCHEPGGCGGVSITRALE
                     TSRKTYRLPNVPGCVPGERVGVVIEDGVALRAALLTYGLPVLGVIAGAALGTVLATPE
                     RGDLGAVVGAVLGLAGGFVAGRGRWGERTSPVRLVRDADGTGCGA"
     gene            27814..29238
                     /locus_tag="PLH85_01090"
     CDS             27814..29238
                     /locus_tag="PLH85_01090"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNB63181.1"
                     /translation="MQRTLGRFIWVMVLLGALMLVRPAWARDLPDVAELAERQGAAVV
                     NISTTQAGRSGVRGAHPNLDEDDPMFDFFRRFVPRGPGGVPRMDPDDRSLGSGFIVSS
                     DGYVLTNAHVVDSADEIVVKLTDKREFRARLIGADARSDIALLKIEASGLPRVAFGDP
                     ARLRVGDWVVAIGAPFGFENSVTAGIVSAKGRALPQENFVPFIQTDVAINPGNSGGPL
                     FNMKGEVVGINSQIYSRTGGFMGLSFAIPIDVAMDVQAQLRQNGRVQRGRIGVVIQEV
                     SRDLGESFNLARPAGALVSSVEKGSPADKAGFEQGDIVLRFDGRSVGLSSELPRLVGG
                     TRPGSRVVAQVWRRGVQKDLTVTVAELPDEKPQKVIAKVMRPEAPPNKLGILVVDPNA
                     EQRRKIGAGVVVDALRSPSARASEIQAGDAILALVSRGTRVDIRSADQFNRVVAAFEG
                     GQAVSALVQRGDVQTFVPLRTLER"
     gene            29228..29479
                     /locus_tag="PLH85_01095"
     CDS             29228..29479
                     /locus_tag="PLH85_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNB63182.1"
                     /translation="MSADRHLTVLSRAWCHLCHDLLDALAPLQVELGFSVEVIDVDSD
                     PELEERWNEAVPVVLAGEGELCRHFLDAAAIRAHFRRIG"
     gene            29546..31342
                     /gene="lepA"
                     /locus_tag="PLH85_01100"
     CDS             29546..31342
                     /gene="lepA"
                     /locus_tag="PLH85_01100"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNB63183.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRIIQRCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALSYRARDGQVYNLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTALDQGVEVVPVLNKMDLPQANPDGAKQEIEDVIGIDATDAIP
                     CSAKTGLGVEDILEAIIARVPPPKGDPEAPLKALIIDSWFDNYVGVVMLVRVVEGGLR
                     PKDKIQFMATGVQQLCEQVGVFTPKSVARETLSAGEVGFVIAGIKELKVAKVGDTITL
                     ANKPAAEPLAGFKEIKPQVFAGLYPVESNEYDQLRESLEKLRLNDASLQFEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVLNDGSVEQVSNPSRLPE
                     ASKMAEIREPIITSTIFLPQDYVGPVITLCNQKRGMQVDMRYHGRQVQLIYDMPMNEV
                     VMDFFDKLKSVSRGYASLDYEFKEYRAADLVKLDVLVSSEKVDALSVIVHRSTSQYRG
                     RELVAKLRELIPRQMFDVAVQAAIGSHIIARETIKALRKNVLAKCYGGDITRKKKLLE
                     KQKEGKKRMKQVGNVEIPQEAFLAVLRVDEGK"
     gene            31389..32225
                     /gene="lepB"
                     /locus_tag="PLH85_01105"
     CDS             31389..32225
                     /gene="lepB"
                     /locus_tag="PLH85_01105"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB63184.1"
                     /translation="MNFALILFVLLLISGALWALDHFVARKQRGAGAPPPWWVEYGAS
                     FFPVILVVFCLRSFVVEPFKIPSGSMLPTLLVGDFILVNKWTYGIRLPVINKKIVDIN
                     QPRRGDVMVFRFPADPSLDYIKRVVGLPGDKVEYLDKKLVINGVEMPQRPDGDFLHPD
                     ALDPSRQGDGKGADRQTRMFSPRFIEKLGDVEHSILIEPDVPSFVSQVVQFPQRENCT
                     YTSRGVSCTVPEGHYFMMGDNRDGSFDSRGWGFVPDANIVGKAFFIWFNFNDLKRIGS
                     FH"
     gene            32252..32599
                     /locus_tag="PLH85_01110"
     CDS             32252..32599
                     /locus_tag="PLH85_01110"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNB63185.1"
                     /translation="MSIIGMALSAVLMGAALLVFLKVVPAVSEYMGVRRAISAVASGA
                     DPTTATVSQLRDAFTKRARIDDITSIGASELDITKEDGKIVITASYSRKIPLVSNVSL
                     LIDFYATSAPTGR"
     gene            32608..33279
                     /gene="rnc"
                     /locus_tag="PLH85_01115"
     CDS             32608..33279
                     /gene="rnc"
                     /locus_tag="PLH85_01115"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNB63186.1"
                     /translation="MKLDALQVALGHRFNRPELLVQALTHRSFGSPHNERLEFLGDSV
                     LNCVTAIALYDRFAALREGDLSRLRANLVRQEALFRVAETLRLGECLKLGEGELKSGG
                     HRRPSILADALEAVFAAVFLDAGFETAKAVIDRLYADALVAIDPASALKDPKTALQEW
                     LQGRRMPLPRYSLADTRGEAHRQEFEVECEIAGLGLKTRGVGVSRRAAEQQSAQRALE
                     RLPKS"
     gene            33276..34184
                     /gene="era"
                     /locus_tag="PLH85_01120"
     CDS             33276..34184
                     /gene="era"
                     /locus_tag="PLH85_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNB63187.1"
                     /translation="MTEHTVSADTPFRTGFVAIVGRPNVGKSTLLNRLIGQKISIVSR
                     KAQTTRHRVTGVLTEGGAQFIFVDTPGFQTRHKNALNRAMNRTVTQVLADVDLVLFVI
                     ESGRFGPEDEQVVKLLPEGARVILVINKVDMLADKARLLPFMERMSGVYPFTEIVPVS
                     AEKGQNTAQLLKAAMPYLPEGEPIFGEDDITDRSERFLAAEFLREKLFRQLGEELPYG
                     MTVEIEKFEAEPGLRRIHAAIIVDKAPHKAMVIGRGGERLKRIASEARVDMEQLFDSR
                     VFLEVWVKVKGGWADDERALKSLGYE"
     gene            34218..34955
                     /gene="recO"
                     /locus_tag="PLH85_01125"
     CDS             34218..34955
                     /gene="recO"
                     /locus_tag="PLH85_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNB63188.1"
                     /translation="MAAKQRVDQQPGFVLHSYPYRETSLIVEVFSRDHGRVGLVAKGA
                     RRPSSALRGTLMAFQPLLIDWSGGGEVKTLIRAEWQGGQPLLGGQALLVAYYANELLM
                     RLLPREDPHPELHHAYGEALRTLAGGEAQEVVLRRFELALLQELGYGLPLEEDVDGQP
                     VRPDRFYAYIIERGPVPVDDAGIEEAALVSGRTLLHMARGDFSDPDILAGSKALMRRL
                     IQHYLGGQVLQSRRVFTELIAFPGEPS"
     gene            34952..35710
                     /locus_tag="PLH85_01130"
     CDS             34952..35710
                     /locus_tag="PLH85_01130"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNB63189.1"
                     /translation="MIELGVNIDHVATLRQARRTWEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDADVRKLRELTQIKLNLEMAATDEMVGIACALKPELAMFVPEGRHEVTTE
                     GGLDIVSQEARLKDAVARLADQGIITSVFIDAEVAQVEAAARIGARVCEIHTGPYAHV
                     FHDAGRDAESPAVVAEIDKIARAGEAIRALGLRFNAGHALNYYNVQPIARLPGVRELH
                     IGHSIVSRSVFTGLREAVREMKRLMREAAERGAR"
     gene            35707..36084
                     /gene="acpS"
                     /locus_tag="PLH85_01135"
     CDS             35707..36084
                     /gene="acpS"
                     /locus_tag="PLH85_01135"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNB63190.1"
                     /translation="MIHGIGTDIVRVARLDEALARHGARFAERILAEPERVAWQAARQ
                     PARHLAKRFAAKEAFGKALGTGVAAPATLHAVRVGHDPLGKPVFEYAPALAAYLAERG
                     LVAHLSLSDEADYVLAFAVIEQP"
     gene            36081..37118
                     /gene="nagZ"
                     /locus_tag="PLH85_01140"
     CDS             36081..37118
                     /gene="nagZ"
                     /locus_tag="PLH85_01140"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNB63191.1"
                     /translation="MKLSHRPPGPVMLDVAGLELAAEERDILQHPLVGGVILFARNYA
                     DSAQLRALTAAIRALRPGLVISVDHEGGRVQRFRDGFTRLPAMRRFGALWDADPEHAR
                     GAARQAGLVLAAELRAHDVDLSYAPVLDLDYGVSGVIGDRAFHRDPQVAAELARALAQ
                     GLAAAGLKAVGKHFPGHGAVEADSHVAIPVDHRSFDAVWAEDIVPFRVLARELGGVMP
                     AHVIFEGIDPRPAGFSPFWLQDVLRGRLGFTGVIFSDDLTMEGASVAGDIVARAAAAQ
                     RAGCDMVLVCNRPDLAVQLLDAWQPQPTADSLARIAALRPAPGGVPGDPLTLARWAPY
                     CEARDAVLALA"
     gene            37137..38933
                     /gene="uvrC"
                     /locus_tag="PLH85_01145"
     CDS             37137..38933
                     /gene="uvrC"
                     /locus_tag="PLH85_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNB63192.1"
                     /translation="MNFDAKSFLRTLTEEPGVYRMIGVDESVLYVGKAKNLKRRVASY
                     FQRTQLSPRIALMVAQVARVDVTPVRSEAEALILENNLIKSLGPKYNILFRDDKSYPY
                     IALSGGEYPQIYYHRGGFQKGVRYFGPFPNSWAVRESLHLMQKIFRLRTCEDNTFSNR
                     SRPCLLYQIQRCTAPCVGKIDAAAYAADVQLASLFLNGRHGDVIDDLSRRMEAASERL
                     AFEQAMVYRDQIRNLQAVLHKQFVESSKDEDVDVLAAVEQGGLVCVNLAMVRGGRHLG
                     DRPLFPANAQGCAPLDALAAFVEQHYLEHPLPGKILVNGDVATLREALEAVVEGKAPV
                     AAPRFENERAWMGMAENNAGLALLARQQVSARGEQRLDALRDALALGDPPKRIECFDI
                     SHTLGEATVASCVVWDGAGMKKSEYRRYNIAGITPGDDYAAMRQALTRRYEKVAAGEG
                     ARPDLILIDGGKGQLGVAHEVLTELGLTEIGLVGVAKGESRKPGLEELVFPDGRAPVH
                     LPPDAPALHLIQEVRDEAHRFAVAGHRARRAKTRNTSRLEDIAGVGPTRRRKLLAAFG
                     GIDGVRHATIEDLCRVDGINRQLAEQIYNALR"
     gene            38949..39521
                     /gene="pgsA"
                     /locus_tag="PLH85_01150"
     CDS             38949..39521
                     /gene="pgsA"
                     /locus_tag="PLH85_01150"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNB63193.1"
                     /translation="MPLNIPNSLTWARIALIPFFVGIYYLPADVIGPDDKNLVATLTF
                     VAAAVTDWFDGYLARSLGQTSAFGAFLDPVADKLMVAAALIVLVHLGRADSLIALIII
                     GREITISALREWMAKVGASASVAVAFVGKLKTAAQMSAIPLLLYDDQLGGFDTRPFGT
                     LLIYVAAVLTLWSMGYYMRCAWPELMKRSG"
     gene            39581..39656
                     /locus_tag="PLH85_01155"
     tRNA            39581..39656
                     /locus_tag="PLH85_01155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39614..39616,aa:Gly,seq:gcc)
     gene            39697..39770
                     /locus_tag="PLH85_01160"
     tRNA            39697..39770
                     /locus_tag="PLH85_01160"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39729..39731,aa:Cys,seq:gca)
     gene            39783..39858
                     /locus_tag="PLH85_01165"
     tRNA            39783..39858
                     /locus_tag="PLH85_01165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39816..39818,aa:Gly,seq:gcc)
     gene            40229..40762
                     /locus_tag="PLH85_01170"
     CDS             40229..40762
                     /locus_tag="PLH85_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNB63194.1"
                     /translation="MKKVLLLIAACVFSLLSVTARADAIFAPDASGITGSTVSLNVSL
                     LSGDISFGGDFTIGYDTAKLTLLDVLAGDPMFSPLRSFTPGSESVSLVNSSGSSYGSG
                     PLFSVLFRILATFPDSTPVDISGVLYDAAGGTNALTTISPLVTVDQRTGTLPEPGVFG
                     LAGAALLAMAVRRMRRG"
     gene            41105..76177
                     /locus_tag="PLH85_01175"
     CDS             41105..76177
                     /locus_tag="PLH85_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNB63195.1"
                     /translation="MAFEPLEPRVLLDGTPSVPITRIDGSIDVAGETDRYGFTLSNDA
                     RIVFDSLTNSNSFTWSLSGPRGDVVSSRAFAQSDADNAQPVLQLAAGDYTLSVDGVMD
                     TTGNYAFRLIDLNAARAIDAEVPVTATLETGYQTDAFAFDAAAGDRFVFDNTGDTINA
                     TWRLIDPFGAQIGSNHGFGDFDSGSLAATGRYTLLVEGRIGAAADATPYAFRVLKAGE
                     KVSTLTPGDVTRGTLASAAQSARYDFTLGETGQYFFDSLTADSRLTWSLRGPLGQTVS
                     PRSFYYSDAYYSTGALALGPGDYSLYVSASSDFSGDYAFRLANLTQATPVNFDVDTAA
                     TLDPANGSLMYSFSGTAGERIALQQTGSGPSQTVFWRILGADGQQIAGVNYFGGFMEL
                     PKLADSGTYTLIFEGDVTASGTTDFSFRMIQCPDVTEAIALGDVIDRSIDTPGQVDHF
                     TFSLASATRAVFDSLSNDYNLSWGLYTADGRLVAQRGFAYSDGWSSGDGLLDLAAGDY
                     QLVVDPYADFTGPFSFRLLDTSAGSAISLGDTVTVDVGHSGRDTTVYNFDGQSGDQVE
                     FNFTYLSGEGGAVYSLFDPYGRRVLGATDGSPQQSRLELTGRYTLLVEGNVFSNNDSS
                     YELFLNYLSNDGGNTLPVGTAINVGDEVTGAVDDLATPVVYNFTLNSETILLFDALGE
                     DEYALNWSLTGPRGEEVSHRAFYTSDGGYYYYGLPPGQLIAGDYALTLYGPANQAFDF
                     RLVDARSTAAPLAFGTETSGTLQPGNAVQVYSIDATAGDVANLVVNSSWNGGLFVYDP
                     DGNLVVGPTYAGSQSGISFSRTGTYLLVLSGPRWQTWDTYYDFRLNLQSSVINDIQLG
                     DGVTGTADSSGSHQRYRFTLTEPQRVLFDSRTGNSGLYWNMSGPQGVERSDTNFYYAD
                     GYLYGNSPVLTLNPGTHFIDVWSTSGASADFAFSVLPVSAAKPIDVNQMVSGTLDPAN
                     GIAAYSFDGQAGDQLNLSALSSSQPYYNMAWRLIGPSGQQVASYYFYNFTPSALPESG
                     KYLLLVEGNNSLSGQLNFSFQVNRPVVVPPVGITLDTTINGNHGTTAQNDRYSFHLDQ
                     STRVYFDYLSGSTNLNWSLAGPRGTIISQRGLYSDTADYSGPASIDLAAGDYIVTISG
                     GATGAYAFRLVNEENKPVIAYGDVVKSQLSPGSEMEMFSFDAQAGDQVFVDTKPGTSQ
                     YSSYIRLIDPFGRQVASGYYFSDIDTVKLAYTGRYSVIVEGLYYYSGNATDYAFSVEK
                     VSSTSQAIDVNGTTGLDPVYVAAPDGDALVLGPYGSAQRANDPALNLTGSLTLEARVK
                     VNSFANGWMPLIVKDDGDSNHRTYSMWINSGGYVLLTSSDQYYDQSVSTNSGTIKLGE
                     WVDIAGVIDRTSGQLRIYLNGQLAVSGSLRTNAAFSHGGPLLIGATNENWNGWANFDG
                     AIDTVRIWNGVRTQTQIDTDRSTAPAPDAAGLVADYRFAASGGSTLVDSSAHGLNGAV
                     VNTLTGMPGTIVGRIDRAGGSAAYTFTLTGPRTFYVDTLLPSRSDFRWSLSGPSGLLV
                     NRAAFTDTEQIVTLDAGDYSLVVDGDGDVTGGFALRLMDLSATTAITYDQVVSGTLTP
                     SSGATAYTFNAVAGDRAYFDALSNSTGNIRWRLLDPYGNTAFSYRGLSDVDTLTLGFT
                     GAYTLLVEGYYGDMTTASYSFNVRRVVDSVTPLVIGQSSGLDPRWVPGKSGNAVQLDG
                     YDWVEAPATDALNLTGSSTTEFWFKADAYSGTWTPLVFKGNGDSNQRQVTVWLNNAGY
                     LHLSTNTNGTWQGVSTNVGSVQLGQWEHVAAVIDRIGNRLKVYINGTLAVDGGLAGGS
                     ALSNSKPLMFGSALESYPRFTGSLDDVRIWNTVRGASDIATDMSTALSGNEAGLVAYY
                     RLDESAGRSVADTSSQNSPATVRHILQSLSGTAVEGAITTPGQQVRYTFSLASAKRLA
                     MDSFTTNSNIYWTLTGPRGTVISNRTLSYSDAAEIGGTSPSMDLGAGDYVLTIDGYAD
                     TTGPFAFRLMDLNAGSNLAYDTPVSGTLDPGSSNTVYHFTGTSGDRLFFNATSLTNAG
                     QTPYVRLLDPRGQQVWSNYFSNIDVFSLAATGDYTLVIEGSAYQTVPIGYGFTLYRVT
                     DDKTSLTLDSTVNGTLAIPGQTDQFSFHLAQDTRVYFDSLTANSSLTWTLAGASGTVV
                     SRNFYYSDSDGLGGTNPFMLLRAGDYTLTVDGSGNTTGAYAFRLLTAASAEAITVGTE
                     VNTSFDPYASHLYSFDAAAGDQVMLDFLDRTSSFYPIWRLFDTDGQQLFYNYLYNDSG
                     TLTLPKAGHYLIAIEPRIYDGSGTARFQVTSQGHVDLPAPPVGDPITLGAQVNGNLTS
                     GTDSKTYALDLSDARRLYFDVLSNTPYVQWSLTGPSGALVTSRYFYSSDGSNASSYGN
                     TPILDLAAGHYTLTVGGSAGAFAFRLLDLVAATAVTLDSSGKADVSGQLSPRSETDAY
                     SFTATAGERYYFQRLQGNYNSAFWRLIGPSGQVVMGPSDQWYDVENVTLPEAGTYTLL
                     IEGPYYYTDTANYSFRISRITDPAPVTIDLGQVVSGSIDSAGQVSHYTLVLDQARQVY
                     FDTLGSANDGAVAWTLSNSLLGSVVNSRAFSQSNAYDRSGNGVLLDLAAGTYDLVVDG
                     AGSFTGAYKFRLLDTTAAATTITLDSTVVANVDPADSTRFYRFDATAGQRVFFDMLGS
                     TGSTPYWRVIGPHGEVVQDVQWGGSDRDVTTLPSSGSYLLMVEGVRNATGTASLSFNL
                     RTVTDKTDTLTLGSTVNASIDAPGQTSRYTFTLGDRKRVFFDALNYNGNVTWSLTGPR
                     GVVVSSRAFGSSDGTTNQPWLDLPAGDYQLVFDAPGDSTGAFQFRLLDVAQATAITPG
                     TVVTGVLSPVAEMSLYTLALQAGDRIYLDSLGVDRNAYDLSWRLLDPYGRIVASPGGF
                     VDVDVTTAAFSGNYMLIVEGQPAAVGTANYSFNISPAPLSKVVPIAIGDQPGADLVVS
                     NLAVTPVSSDVHSGGQVVLSWDTVNAGTLPTSGSWKDQIVVRNSRGELIYTQLVDHDE
                     AVDGPLASGAKQSHSLTLTLPDGNRGAGTLSFRVTTDVENTVAELGSGGAAELNNAAT
                     VSVASTLSPYADLQVANLNVSPAAGWAPGSQVTLSWRDVNAGVGATSGGWTDSIVVRN
                     LSTGRTFYVGSLAYDATANGDLGAGDGRDRQLTLVWPADAQGIGRFEFSVVADAQGQI
                     FENNANDSAESNNTAALTVTSAPDLVVGNLRTTPSSTESGSSVTVQWDTVNQGLAGTL
                     GSWVERVTVVNPATGETLVDTRLTYDPSVANAGNIAAGANRNRSYTFTLPEGQRAVGA
                     LNVTVSLDYNGQLVESDETNNSANVVLNVAKKKYADLAPVSLEAPPSARGGDTVTLRW
                     TVTNQGDAPTGTQWTDRIVLSADGIYGNADDVVLVTAPHFGSLAPGESYTQERQVVLP
                     QRVDGAYKLFLRTDVNAEVLEPDTRANNLFGPKAINIAAPHADLVVEAVSAPTAAQSG
                     DSVKVSWRVRNTGDSTTDSSQWKDYVYLSSDNQLDASDILLAQVDHTGQFLAVGDSYT
                     ASANVFLTYGLTGNYSFIVRTDAENRVFEANFETNNTTAALQTTKVTPAPVPDLQVSA
                     ITLPAQIVSGQTAHVAWTVNNGGGGTAKGPWTDRVYLSTDGTLNSPTFLGDVAHTGNL
                     DAGGSLSSSLDFIVPALADGNYRIVIVTDVNGQIYEGGSGSAGENNQFAATVPVALVH
                     PDLVPLQLDAPSTAVSGNRVTLTYTIRNDGTGAAGGDWIDRIYLSRDTTVDASDTLLA
                     TRAVSGPLAAGQGYGGSVDFDLPLGAQGTYYLLVAADANNQLPERNAEGNNVASRQLD
                     VTLDQYAQLVTTDVQAPTLTIGDPAQITVSWTVANQGTGVGRTSTWTDALIASTDSIV
                     GNSDDKVVGEFVHTGGLAVGDSYSRTETFLLPAYFQGRYHFFVKTDAKNAVFENGNEA
                     DNAKESPQIFDVMSIPYADLVVSAASAPTTASSGQTMSVTWTVANTGIGPTDIPGWND
                     EVVLTSDAAGNNVVANLGSFTHLGVLAAGDSYTRTQDVSIPNGLSGSYYVRVRSFGPF
                     EFVYTQNNTRVAGPVQIALSPSPDLQVTDIQTPSTANEGDYIDVKWTVTNNGAAKADG
                     SWTDMVYMRKVGQPNDPGIYLGTFSFDAGGLDAGKSYTRTERFKLPARTEGVYQAVVV
                     TNANANSGGVYEYGTAAANNTTADDQILQVALLPRPDLQVGTVVAPSSASAGAAVSLR
                     FSIINQGTVPTATPHWKDNVYLSLDNKLTGDDLLIGSYDNGAALNPGEQYATESGSLQ
                     IPIRFRGDVFLIVQADGSGSVDEYPNDANNITAVPLYVNPYPLADLVTGGVVAPAQAV
                     EGANIEVRYTVANKGSGTTASNSGAASWTDTIWLSRDKTRPNAGGNSAVMIGTVQHNG
                     ALKVGESYDVVTNVNLPSHLTSGTYYVTVWSDSYDVILEDTLASNLNPDDPHELDNNN
                     YKARAIDIIGGFEPPPPDLKITALTASPQASTSSDLTVNWTVTNAGAGEASGKWYDVF
                     WLSDKPDLNASGAKQWYLGSILHDGGTAPGASYSASATFALNPAAAGKYLIAYTDFDP
                     YVGKPYDVVEGNEDNNTRVTDTSVTAVPADLKVVTIAAPSENFSGEKTTVTWTVKNYG
                     GDVWSGTRYWLDSVYLSPDPVFLPERATALGTFVHSNKDGLAAGASYTQSADVVLPRG
                     IGGNYFLYVITDANPYDPRFPNGETDQGDNAYFRDVYYPGSAYEGVGGEQNNRGIGNI
                     PVTYREPDLKVTGLVVPPDAQSGKTITVTYTVTNIGNRDTRENAWWDRLFLSKDGSLD
                     TYDLQLSEARHYGILKTGDSYTGTATFKLPDGISGNFSLLAFTDSEADARLSWWNSNI
                     VPEYRGVGIDANSDSVPEFRGEGNNITVAPLPVVAAPLADLQVTTVTTQAHITVGNDF
                     TVTYTVANLGGADTPPEQAEWEDYIYLSRDPLLDLDSDRYMTTVRHVGGLAKGASYTV
                     NQTLRAPLDVTGAWYVFVVTDPARGSGRGKVFEGGLERNNATPSTPAIFEMPPPSDLQ
                     VDDIVFGGNGQSGENIHMEWTVSNHSTNPTSTSWTDAVYLSDDNTWDIGDRLLGKVTH
                     TGVLGQDGSYTASLDALIPPTKPGQYRVIVRPDIYNEVYEGPYRSAGEANNFTTSPNV
                     LNVTVNELHLGVYEDTTLSTGQSRLYKLTVGQGQTLKVSLTTGDQDAANEIFIRYGDV
                     PDGFHYDATYDNPLQANQTAVVPFTKPGDYYVLIRGHAETQANTPVRVLAEVVPFAIT
                     SVHVDQGGDSRWVTFDVRGAQFADNAILKVVRPDVAEYEPVKWDVIDSTWIRATFDFT
                     HAPLGLYDLEVINPSGDKAIIAYRFLIERAMETDVTIGLGGPRVLAAGETGTYGVALQ
                     SLTNVDTPYVRFTFGVPEMGNNQYVYNLPFLKYYNDLRGQPDTGGADVPWASLDSATN
                     TTGQILSSGYAYDVIAGGYAGATFNVTTYPLLKALSTLNWDALRVGLYEMYPDLEKIQ
                     ALAGGPESMQTALPAYWDLWQQANSEDGLPDDCVIPFIPYRFNIVGAATPMTRDEFIA
                     DQTTEALKLRAAILGDTDLDAKIAATGTDDTTLAEKRAAIALRNLASDADGWVNAYLG
                     ALEQVGILRPVDEAPPIRQNTKVMSLMSTLATGILLGPVGDQIRTTGDLVEFFAKIKT
                     WYGDTPKTLAAIDHYEHRESDCLDGEIPVPALPKYADYNLNLSHPTYFETFNIFVPYV
                     GFNKDGASAATIALPDYASVDASNQLTALDFSRLYAQAISDSQLASISGPQGYGVDQF
                     LPVGQALPYTVKFENPAAASSSVTEIRVASALDPSLDPTTFRLGDIKLGDVTIHIPSG
                     RALFQGDFDLRNSKGYILRVSAGIDPTTSTASWVFQAIDPDTGEVLQDATRGLLLPNA
                     ADGRGTGQLGYTVQPKADLPSGTQISAQAVVRFNAIAPQETQKLTQTLDTQAPVTTLT
                     ATPVTAGGNDYEVRWSSADEDRGSGVAHVTVYVAEDGGDFKIWLRQTTDTFGVYSGKA
                     GHTYEFLALATDNAGNREQPPGSVQAPDDGSGANLGGLPSFGETTQDLPPPPAPATPP
                     TNPLFIEAQSQTPAATPLSKLPEFTEVERPFTAQAFATDFVSSGAGVGALAMLELPDG
                     SVLASGGPNRGWLYRIPHDGGKALTPFVELDDPIYDLALDDNGQLWATTGGNDLLKLD
                     LATGAVLARYGTGITQALAIKAGTGEIYVSSGDGIEIFDPVKATFRHFSDVRVDDLAF
                     APDGTLWGTTWPDRGDVVTFDKRGRATVQVRLDGEVDSIAFGQVGTRLEGLLFISANS
                     TKGEQGAALTMVDLVSKRSVEIARGGSRGETVVATKDGRVLVAETHQIDVISPIVAPR
                     VVATNPPDQAVVTLPLTTLTVTFDQDMYAGSATDSASVLDPAVYQVVRQNGSLVDIAS
                     VAYDATTRRVTLTVAGLDPDLYDLRVLGTVRSSAGMALGGTYISSFVAVQDFSSVVSL
                     QFDRVRSDRGTGVVSYDITVTNTADYDLLVPAALVIDPARFFQGRPMGAVSPQGANGL
                     WYIDLSGKLPGGVLKAGASIATFTVGITNSASQQISLGHGIYAVPYPNQKPAFTSTPV
                     TAATAGQAYRYQAVAQDPDGRVITYLLVDGPAGLTVDSTTGILSWLPNVASPATSTVT
                     LRAYDTRGGFDTQVFNIEVAGANHPPNVSDLPAEIDGKEGQSLLVGLAAEDVDEDFLH
                     YYADNLPPGAVFDSQLNALVWTPDFTSAGTYTNVKLIVSDGKSTVTRSFNIVIAPDDA
                     PPVLSPVPERTLREGDPVRLQLHATDADSGDTLSFYADVLPAGAFLDPNTGVFQWTPS
                     FIQAGDYSLKFGVSDGTKRTETTVKFHVLNVNAAPVFDELGSWISFEGQTLAFRAFAF
                     DPDNPGYVAQDRLPSGTLTELEGAAATVSYTVSGLPDGASFDPVTSMFKWVPRYDQAG
                     SYLVHFTATDDGNGTGTPLTSDIVVPITIRNANRAPEMAEIPTQTVQRDAVFELPVTA
                     TDPDGNPLTLTVKGLPAFATFTDNGDGTGRFVIAPHSGDRGDYEIDLTATDNGDGQGA
                     LYAQSLTRRFILTVVSASEAPVIAPIGDKLALVGQKLSFYINAKDLDQDALGFTADAA
                     PAGLTITPQPQYGRALVEWTPDSTVLGQTFTVHFKVTDNGNSGATAPNTTEQVVRIKV
                     RDSNAAPVLSPIGNLSLKEGQPFSLQLSAVDPDGDPLTYSVTNLPAGASFDAVKGLFT
                     WTPNIFSAGDYPNVRFAVSDGMAESAETVTISVQNTNQAPILVSLFPQTGREGAQVQF
                     QLTVGDVDGDPVVFKALTALPDGSRFDSRTGLFKWTPTYDQAGDYSFTFEARDPAGAT
                     SQTTVSLAIANVNRAPVLTVSNHVALLGRPLSFQLGGSDADSGTTLTFSATGLPEGAT
                     LDPTTGVINWTPGAGQAGDYLVMASVSDGMTSTALPLVIRAALTAPAPSVIIENTPSF
                     PAVPGQDVLVHVIAESFGNIAGLRLFADGVELPLDEFGRAHVQATQVGKIALRAEATD
                     VDGLVGSTTSVIRVRDPQDKSAPTPSFASQLAFARLTDLTAITGSTGEANVDAWTLEI
                     ARYGDEHYTTLASGDGAVNNAAFMQLDPATLANGMYTLRLRVTDLGGRRGEASVDVEV
                     DSATKTGRLQRNEADFSWSVGGHTLDFLRSYDSLNTSVAGSFGNGWRLSFRDVDLQTD
                     VALTGREDAGELGTFQDGQRVVLTLPSGERAAFTFVPQKHSIPGQTWYTPAFQAESAS
                     GWQLVVPDTKLQRAGSSYYDLVSGVGYNPANSGDTDYILVAPDGTRYSIADGRIDDVR
                     FTDGVHVAVSDSGLVASTGETVQFTLNHAGAIERAALSDGRQYLYDYDAQGRLVSVRN
                     LEAATSLRYGYEAGALGRLTLVTGSGAGALYTYGPSGVGQAPISGDLGSSVNYLATPV
                     NGSLIAGEVDHYAFTLRPSELASTASGSVYLGIVVEGHNGLAPVLPVMPGLTAIASSV
                     VGNRAFALFKLDAAGLAQIDVAGSGVGDYTLSLFVAGDANRDGKVDGADAIAIAAAKG
                     AVRGGAGYSEALDADGNGVIESGDLQLLMQNAGFTPNRAPVVVAGTVKTHVDLDVNAP
                     VSTFVKDPEGDRTFFTLLGSTHGTAHLSGDGQSVVFTPEAGYIGTADFTIRADDGFGQ
                     TIQTIAVTISNAALTRLDFVDRNLRLAVGEGRQISLIGDFEDQANVELPASYVTLMST
                     DGAIGSLVAGGRINAVGKGSGVLIAERAGLRAATAFTVGVPTDTVQAYLYAGGMTVYP
                     GAVSLAENVGKRQILANLYGAMDLAPASSGTHYYVSNDQIISVSADGLISARQSGEAT
                     VTVINGGAETIIPVKVEKPFVGPTVLGDLGGIVQGADGSIVAVAPGALRGNTTVSIEA
                     VKAADLPAGMPDGMALAGAFKLDLGDRKLDTPIQLAIPTTGIAAGTKVVFMRYAEIPM
                     PDGSKQPIWLQEETGVVGADGYARAMSPPYPGVTVSGTYTCGIAPDASFSQVRGQTYA
                     TFPVGINDGGSSFAMLSSLGGGAAMGAAFGLTAGFMLTCLIGYHELQIISVPAQGLPT
                     VTKVGVQVDPGKVSDNRIEVNNPYSPSTSPATEPFVSSARFLFKDDGSGNVVPFVEIK
                     GDRFTYDNPAAPAFAKKGSQIADLKVVFETPDGHTFEVSPSATSTDGDVFVEVPQTVS
                     IGTAKIFVRRPIYYANGGTWDDLEQLDSNKVQLSYDARYIFGGMSGNQVFAIDSQTQD
                     LVARINVSGNFPAPRAMAISSDNTRAYVTLRYNGAVALVDTIALQQVDVDGDTANGIS
                     SIPLLPGAQPFWIAIAKGGKKAYVSDEVAGRVYVIDIDPSSSTYNTLVKTIDVNPAPS
                     GLRGMAINANGKRLFVAAPDKALFGRPGGGDGKVFVIDVEEGSSTYGQVVATLDAGSE
                     PYGVSATNDPNVMMYTNRLSDSKGVVIVKGDVGNYTQTTVTLTLGAITDWFDVNNASS
                     VVLSKDTKYAFVVGYNTYIQDVPSHDPNYDPNHPAGGNIGIIRDPLGPNPTLVGATRE
                     IPMSWPDNAVLSPDGNTLYAAFRGQRVVMAFDVAKMIAVVESGSPDLGTTAVDDIDPS
                     IDVRANFKMLKKGGLFTDPVFGVPPGATNGPIGVGFNQGLVTQGGASLELLAPDASTS
                     NLNPNFSWMVKGFEGAKARLYVSTFRGSDGLFPTDLGSTDQNPNRIFSSDLLDASSAD
                     STGAIFIKQLPANLKLTAGQQYYWGVEVVSPSGTVVARDWRVFKTEAFKDPSKPYSSV
                     TVLTHGFQLSPTQPANLADALSSFYQMAETIAKDGGEGVAARYQKSTGHWIIFYDGAN
                     SGALVGHDLVDALRMPSLNGRPIALVSDWYDESDFPTDGFSEAAADTFYSSLAQLNEE
                     SGGKIFQSPIHLIGHSRGTVVNSELAQRFGVYAPTVSLDMTALDVHDFNQPSLDIPIA
                     QYIQAAGAAAGAVSGLVGNLISLWGMLPTNAVIPTGNFLDPDVQRWSNIAFADAYYQV
                     VASNTPMGFGQIVTTPNGRYVPNFDYSVNLTGRAGFTRDDGLISASLLASGEFRAGGP
                     HSRVWRWYGGTMDLSATTFQAGGEPVYRRLYDKTKTEFQGVTVPWYNAQSFALGSGPW
                     EGIGTGWAFAPQGGVTYSGPMSATKVSLATDNTEAPNPSGMAVPSIFNGDFEEGALNR
                     SWLLQYSAGGLATRLTGDLPGWSFHGGSFAKTRTLIDGDRLVTLADGTHAAMLDNDNY
                     RIEHNRMVVPDWAEFLTVDAKVLTGKAGEKLLVRGYLADSSVPIELGSIALDNTGTNF
                     QSYTFQVPTAFRGAGRLLEFSMSSGATADAEVLLDHVQFTKGLITDSTLDPSDKAVFF
                     TDTTTGPTTGLSSQWVDLTNPFSQAFTVTVTTDPNSFLVVAGTSGDVVLNDNNAHDPL
                     YTDYVLAPGAHLKLNLKSKFNADFIKTYKDADIPIQKTNLYFTITLANGSIRDEAVDL
                     FYMPDYADGNATDGLWTFMDTMDGKTRTLSFDNAAHVELKETSDTYNLFGVSQSGGIV
                     QGLTYTGRYVNALPDFATYQVEEGGRKLGTIKVAGYSVPKQTVGVSLATLRTMVQNIR
                     TEAENYKNNPLTAVVHDPTGFAAGKYNIFLNLFPDAVITDAQWQDVVDGFNEWFGTGA
                     SAIYQNNIAQTEFSYGETGLNPIVMINQPWVNTVAKPDPNDDASRSFAGLDFAYNTFE
                     STLTKAGAGAGSFGGMVIRDDISIASKRYLLSRVVNPERAGAMGGQSAMTLVVDGILR
                     AYLSVGFTRKDMGRSYGWVVGHELAHNMGLFDEYIYSPFTNAPRDGGANFMSNANNIR
                     VSSSQAAALHLAMDNPDTTVALSSTDALLSWYLDLDALDKSNRAGRQVLNLSPLAGDG
                     EGSTDPLGGLLYFVSQNSDATSPVTSSPILISGASHDGIVNGQFSVADPAATDYGWIT
                     EGDAKVSAGAGVLTEDPRLASRFAQTFVVPADAATLRFQLVSSHFVANGDGPPDAFEV
                     ALIGADGKPVGGTLPLSGSDALFNLQADGTVYAADQVSAAGLTNRNGGTFPSGRPITV
                     SIDLTGVAAGTTVTLYFDLLGFGEAASSVTVDNVRLLKVGEVEDQPPVANADTAAVDE
                     DASVLVNVLANDTDPEGKALTLVSADGATHGAVTLENGQVRYTPSANYFGADSFTYVV
                     RDEAGNTATTSVAITVRPVNDAPVANPDSVAVLSGGSVLIDVLANDTDIDSPVLTITG
                     ATGASHGTVAIESGKVRYTPDAGYAGGDSFTYTITDGDGGNAGSSVTVDILVHNTPPV
                     ANADTAVTDEDTPILIDALANDTDAEGQALSLVSVGLATHGTVSLDAGQIRYVPDADW
                     FGADSFTYVMQDSVGAQASATVNVTVNSVNDPPVANPDSATVEAGQSVLIDVLANDTD
                     KDSAQLAVTAVSGAAHGTVVIESGQVRYTANAGFTGADSFTYTVSDGDGASVDGSVAI
                     TVQLHNTAPVAHADSAVTDEDHSVLIDVLGNDSDAEGQVLSLVSVTGATHGTLAIEGN
                     QVRYTPDADWNGADSFTYVVADSLGATAKGAVDVTVTPVNDAPTLDALADAAVKEGDS
                     FTVTAVGHDVDAGDVLNYALDAAPQGAKIDPLTGALTWKATDGDAAYAFTVRVSDLSG
                     GTTTRSFTVNVANVAPTLQAGGLPAVYAGQSYTLELSSSDPGDDTLSLWHINWGDGQV
                     QDVAGNPGQITHTYDGSVLGQVQIRATATDEDGSYALDPLAIAVLPVPLQVRSFTSDS
                     NGFAVRFNDPFDASVINLYDSTLSNLGVSDVVLSGSGGSLVKGSLVFDADGKGLRFLA
                     TGSGLAADTYSLVLKSGPLAFHSAFGALDGNGDGTGGDDYTTRFTIGPVPALRLSLPD
                     FVRGPGQSVDVPATGKYLPLSLSSAGDVRNLSFTVRFDPSLLAITGAVAGAGLPSGAS
                     LNVDTSVAGQITVTIASATPIAAGKLTLVNLVARVPDTAAYGAVEVVDIGNVFANQTA
                     ADRADDDALHVVGYIGDGNRNAKLDKDDVTLLQRNSLKLDSGLAAWSYIDPLLIGDID
                     GDGKLTTADASRVAQKIAGVARPEIPDVPAGLKVNFATAPTPPTAPVIDFGSSFAGFT
                     VGSSDPKFKKENWKKAFVTNMATSSTNPNANLKVTVDASVQSTTPA"
     gene            76217..76501
                     /locus_tag="PLH85_01180"
     CDS             76217..76501
                     /locus_tag="PLH85_01180"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNB63196.1"
                     /translation="MSDNAAVPETPEGQRIRMNTASLKSTYCNMVNATTTREEVVLNF
                     GVNESWDRTGGDYEVKLEHRVILSPFAAKRLSQMLSKLVDEYEHRYGELK"
     gene            76590..78437
                     /locus_tag="PLH85_01185"
     CDS             76590..78437
                     /locus_tag="PLH85_01185"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB63197.1"
                     /translation="MHAASRSVSAAPADAAVPVAPDALWGAFVSADNAADFCKSWLAL
                     QCSQVGDVRAGLLLLEQGGGTFVPAGVWPTDQTDVTYLAPGAQRCLSERRGTLQRGLE
                     GALVVVAYPLEVDGRIHGAVVLDVAERPDAALQDVLRRIHWGAGWLESVFLRRQAQDE
                     ALALTRARAALDVLGVAAEQARLEDLALAVANDLASRFACDRVAFGVDRAGRARLVAL
                     SHSAFFEKKSQFVVALENAMDEALDQRRSVIWPTDTDAAAGGIAIAHRDFAAQRAVCS
                     VLLSSRGQGIGVLTFERNAGEPFEPDEVLGFEAIGALLGPLVESRIELHRWFAGRLAD
                     KVRDGWRHLRDPRRPAFRAAVTVIAVLLAAAVLIDGDYRVSAKSVVEGEVQRSVVAPF
                     EGYIREAPIRAGMLVKKGQMLARLDDRDLLIERQRYSAEREQHERRYRDALSKHERAN
                     AHVSLAQMAEAESQLALVEEKLARAEVVAPFDGVVVTGDLTQLLGSPVEQGKLLFEVA
                     PLDAYRVILKVDDRDIRYVRAGQGGRLVLTGFAGDALDFKVHNISMAQAEEGKNLFRV
                     EARLDRGDIPLRPGMEGVGKIVVGERSYAWIWTHRLFDWLRLQAWNWLP"
     gene            78450..80597
                     /locus_tag="PLH85_01190"
     CDS             78450..80597
                     /locus_tag="PLH85_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HNB63198.1"
                     /translation="MAGSFLSASWYRVAGLRPQLRGHAQVQRQRFRGKPWYVMRDAAS
                     GKIHRFTPGAYAVLQRMDGRSTLDVIWQDVAAAAGVDAPTQDEVIELLSKLHSGDLLQ
                     TDLPPDVAELAERGARQKRQKRLQSFLNPLSIRLPLWDPDPFLNRSWPWVARLFGPLG
                     VILWLLAVVPAGVLVGMHWDELTGNLGDRLLATDNLVLLFFIYPAIKFLHELGHAWAV
                     KSGHGEVHEMGVMFLVFAPVPYVDATASGAFRSKWRRALVGAAGMLVELFLAALAVML
                     WVLAEPGFVRSVAYNVIFVAGLSTLVFNGNPLLRYDGYYILADLIEIPNLAQRANQYW
                     QWLAKRHLFGAVEVERPAATPGERRWFLFYGPASALYRLFVTISITLFIAGEFFFIGV
                     VIAIWGAITMFGVPVGKMLGYVWGSAELQRHRPRALWVTFGGLAALLLFLFVVPMPLR
                     THAEGIVWVPAQAELRAKGNGFVERVLAAPDAWVQAGDLVIVTRQPDVDADVTQRRER
                     VRQLEVQYAAQMFDDRLQAAVVAEDLGRERAALARAEERLDELLLTAGVSGRLRLSRP
                     DDLPEHFVKKGELVGYLLAGPPRVVRAVVTQDDIALVRDRLEAVEVKIADRLAETYPA
                     QVSREVPGAQDQLPNRALAMEGGGLHATDPRDPDGLKTLERLFQFDLTLPDSVGEVQL
                     GTRVYVRFIHRAEPLADQWGRRLRQLFLSRFHV"
     gene            80590..82599
                     /locus_tag="PLH85_01195"
     CDS             80590..82599
                     /locus_tag="PLH85_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB63199.1"
                     /translation="MSRAVLSTWGVHAFAPGLYPERQDAKPGKLDRFMGGLLGRWARH
                     GSALRLPPVRRFAEQVIAHEGELAALDEATLQARVAPLRAALAASGLDDANAAEVFAL
                     VREVCFRRLGKRHYPVQLMGGYTMLNGGLAEMMTGEGKTLTAVLPAVAVALAGVPVHV
                     ITVNEYLAQRDSEQLAPVYGFFGLSVGLVTPEQEPPERIVAYGCDVCYCVNKDLVFDY
                     LRDRITAQRIKAGARALLEGWLGRSAPVGGSGTLLRGLYYAIVDEADSVLIDEARTPL
                     IISAEQDDPNGRAFYEQGLAVARSLNADEHYRLHLKERSIELTEAGRAAVAEASRGLP
                     GLWQVSRAREELVEQGLTAEHLFELDRHYILAEGKVQIVDEYTGRVMADRSWEKGLHQ
                     MIEVKEGLELTKRRDTISRITYQRFFRRYLRLAGMSGTVTEVAPEMRTVYALDVLRIP
                     PNRPVQRRDLGLRVFARSEARWDAVLASARAHAAAGRAVLIGTRSVAASELIGARLAA
                     AGLDHEVLNARQDAREAEIVADAGQPGRITVATNMAGRGTDILLTPEVKAAGGLHVIL
                     TEYHDSARIDRQLYGRSGRQGDPGSYEALVSLEDELFLAHAARLVAWLRVRTAPDGEV
                     ARRFGPLLRRFAQGAAEAQNARIRRHTVDSDNRMEQALAFAGRGE"
     gene            82602..83504
                     /locus_tag="PLH85_01200"
     CDS             82602..83504
                     /locus_tag="PLH85_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB63200.1"
                     /translation="MKRRTTAGVVLAVLNTLGAVSAARAGEFDCMIEPRKTVDVRAAS
                     EGLIEKIWVDRGDMVKAGQVLVTLDSGVEKAATESARYRATMEGKIRTGESRVEFATQ
                     KYNRRDKLAGQSFVSMQDRDESLTEKRLAESELIEARDDRKLAEIEYRRLSEQLRLRT
                     IKSPVDGVVVDRLLHAGELADNRDLRKPILKLADIGTLHVEALLPVDAWGKLAVGQSI
                     EILPESPVGGRYTATVKVIDRVMDAASGTFGVRMELPNPGLKLPAGIRCKASVPGVTG
                     RTVLAAPPKKPAAAPTAPGAPASH"
     gene            complement(83549..85186)
                     /locus_tag="PLH85_01205"
     CDS             complement(83549..85186)
                     /locus_tag="PLH85_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNB63201.1"
                     /translation="MNKKIVATYLALTGCALQAFAQGGALQGPLPPQVPAAPATDLTV
                     RPPASPAVATPAGPRVTLRDITLSGNTLLDSTTLQAGLGPVSGQSFDLAGLEALAAGI
                     ERQYRAAGYPFTQAVLPPQDLKDGTLRIQVVEGRYGRIQATGTDPLPASAQPFLDFGL
                     RSGDAIENARLERTLLILDDQPGMKIRPVIQPGDRTGEADLSVNVERVSLVGGEVGLD
                     NTGARSTGQNRARGAVFINSPFRYGDKISINALATDQSMLLGSLDYEMPIGASGLRGQ
                     VGYAYTNYQLGAQFAALDAKGYAKITTARLSYPLIRSQASNMLLSAGYQHKNLEDRYD
                     STGLIRNKHSDGTPVAIQFDRRDTLLGGGVTYGSLTWLPGSLSLDPGMDALDRVTART
                     AGHFNKLNLDVARIQQLTSSVHAYARYSGQWADKNLDSSEKFNLGGFYGVRAFPLGEG
                     VGDQGWFTQLEIRYDVGVVTPFAFYDAGHVETNAKPWDVNSASTRRLAGSGVGVRSLR
                     DGWSLDATLAWRTQGGAPTSEDVDRNPRLFVMIGRRF"
     gene            complement(85197..>86144)
                     /locus_tag="PLH85_01210"
     CDS             complement(85197..>86144)
                     /locus_tag="PLH85_01210"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HNB63202.1"
                     /translation="TAGSALDGSGATNTASLAPRAITVAADNQEKTFGETDPALTYRI
                     TQGTLLAGDSLGSLLRDSGEAPGTYAITSASLHNGNYAITAFDGMLTIVPPTAPPPPP
                     APIIVPAFIDNGTSALATGTPPASFGGLNYVPAGGQAAAPRANGAAPGGATPSTLNFV
                     SGTPANPGSAPADPAGGTAAGGAGPDQRVAAGSANETPAAGRSTLNFVAGDAPDARSP
                     ARSDARTDAADRPPQERSAPTAAENEVPLGERTTVTYVSSSASGEAAAGTEAGKRKAA
                     QELNVNNVTVPSSSGPLDVFVIDSGINPGRSATLQSLTN"
     assembly_gap    86145..86244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<86245..90325)
                     /locus_tag="PLH85_01215"
     CDS             complement(<86245..90325)
                     /locus_tag="PLH85_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNB63203.1"
                     /translation="MNHIYRVVWNRALNIWQVVAECARSAGKTRHARVGSVAVLASLG
                     AGLAAAAGPLPTGGSIAAGTGSISQNGNSLTIHQTSNKLAIDWQGFSIGRDNTVNFVQ
                     PGTRAVALNRVLGSDVSLIQGALKANGQVFLINPNGVLFSPTAQVDVGSLVASTRDLS
                     TSDFLAGRYVLDGAGSNAVINQGRITAAGDAGQGGTVALIAARVSNEGSVNAPAGNVL
                     LAAGKRVTLDLGGPLKLQVEQGALDALVSNGGAIVADGGFIYLTARAADQLTTATVNN
                     TGLVRARSLATGERGEIRLIGDLAHGTLNAGGTLDASAPQGGDGGFIETSAATVNMAD
                     TLAVTTLAPHGATGTLLVDPNNYTVAASGGNATGASFTSLINSNNVTISTASYGTAGG
                     SGDIFINDTITKTAASGTTSLTFLAERNIAVNSSITSTGGPLNITLSAANAAAGTLGG
                     VRVASSQTLNSNGGDILIGGAGGSVSGAQTPSHGIGYALNYSSSQEAVSIGSSANILS
                     RGGNITINGNSTQRGGGSSDTTAVHIGASAVIDSGYYAPGATDASSGGFINISGKYTG
                     GVGTNADKVFGVKIDNPSGNAGQTTLAASTTTGSIRIEGSSTYDTPGSYALNMATNGL
                     AGNIYFNAYSVADLIFILNGDLKGVSFTYSPPTSGCRTGYPNCGLLSVNASANNSYLY
                     ATYNAVNMATLPIYVSATLSGTKVYDGTLTASNLSFSNVTVMDPDGSGYSSGNVTSAT
                     YVTPSANVGAYTNLSASALSRNYSANGNNYVLGYNFAVSPAYTVTPATASLSAVKTYN
                     GDASFSNAQITASGVNGETLSLSGSGPATATSSAVADNAINYLTSLGGMTLANGSSGT
                     PGLASNYTLPALSSRSAQNTATINAFSLTVLGVNGSKNYDGTTTFGTNQLNLGSGVVN
                     GDDVHLAGSADVASKDVGTYTQWAASSLALTGSNSTNYTLSGGAVSAVINRAPLGVAL
                     NALYNGTTQFTSSVGATNASAGTINSGGTLTVSGLAGSERLTGVTVSDASVDAITAGK
                     YVTGVSGSNGFLAGNYALYNGGTPAYHVAQSSGAITTLNNTVSISPKPLGVYATPTYN
                     ATAQFTAVSGVADGGTGTLATGGSVSVSGLVNGQTLGGFSVDSPNLVSAGKVTSVTLN
                     GGIDARNYVLNGSTYGTATPTPGSAADGSQATNGVSMSAAPLGVTVGGTYSGSTSIVP
                     TSFSTHGLLGSDTITQLSGATIDNSNVSANGSNFVRSVVVGSGTADIRNYALAPAYSA
                     VVDAGGNATSVTTNVASLGRAPLGITVSGTYNGTTSIVPTSFSTYGLLGSDTLTGLAA
                     ASLNSPNVVANGSNFVTALTPSGGTANLANYQINPAYNGS"
BASE COUNT        14928 a        29949 c        30018 g        15481 t
ORIGIN      
        1 acctgcgcat cgactggccg gccggcctgc gcgcggcgga gcgggcggcc ttcaccgccc
       61 atcgctttca ttcggtgttc ggtgatatgg ggacctgggc cattctggcc gaccagggag
      121 cggtgggctt tccgagcctg gcctgcgcca tccctgcggc gctggtggcg gccattgacg
      181 aagcggcgca gtcccgcacg ctgcgcgtgg ccagtcttca accggcctac gtgagcgact
      241 tcaaccgctg ccgcggtcgt tttcgggacg acggtgcctt cgcccgtctg gagaccgcgc
      301 ggatcactgt cgggctgtgg ggcgacggcc ggtggcgggc gttccgcagc catccggtcg
      361 aactggcgga tgcggcggct gcgggccgct gtctgactgc gctactgaaa acgctccccc
      421 ttgaggatct cgtgccggcc ggcacgctct atacccgtgg cggtgacgct gtcggtccgg
      481 acgcactgcc ggctggctgg cgcggcgaag ccgtggagga cgcgccgtga gccccagaac
      541 gcgctggcgc atgccacccc cgttggggat cgactttgcc cgccggccac gtcggccgag
      601 cgcgtttggc gggctactgc tggcctgtgg tgtggcggcc acgatgctcg tgctgcgcga
      661 acacgggcaa ctggatgaac gggtggccgg ggagcgtgct cggcttgccc agttccggca
      721 cggcgtggcg aagactgcat ccgtcccgtc tccccccacg gcgcccctga gtgagggcga
      781 aatccggccg gctgtggccg tcgctcgcgt gctgcagcga gactggctgg cacttttcgg
      841 tgcgctggaa actgcgatgg ccgaccccgg cgtggccttg ctgtccgtcg agcaggatgc
      901 ggcccggggt ccgctcaagc tggcgggtga ggctcgcaat ctgcccgagg tctttgccct
      961 gattcaccgg ctggagtcgg ggggcgtgct gcgggacgta cgcttggcca actatgactt
     1021 tcggtccagt ggatcggtgc aggtcgtcgg ctttgtgctc ggcgcgcact gggagggggg
     1081 gccgtgacat ggcgtgatcg cgttcggcag tccgcatggc gagcgctgcg caacgccggt
     1141 tgggcgggtg gggccggggc agggttgctg ctgctcgcgc ttgccgcgga tgggctggtc
     1201 aatcggacgc tggccgccga agcggaggcg ttgcgccagg aggcacgcgt cgtggtcagc
     1261 ctgcgggtcc gtccgctgtc accggaggag tctgatcggc agcgccttga ccgtttctac
     1321 gcgagctttc cggattcagc cgggttgccc gatcggctga tccgcatcaa cggctatgcc
     1381 ctggcccgcc aggtggcggt ggaccgggcc gagtaccgtt ccagcaagga gcccggtacg
     1441 cccttggagc gtgtcaccct caacgtcccc gcgcatggcg attacccgac gttgcgggcc
     1501 tggcttggcg acgtgctgac cgacatgcca gagctggcgg tcgagtcgct gtccctcaag
     1561 cgcagcacca ttgccgatcc ggttctggag gctcaggttc atctggcgat atttctgaag
     1621 agtgtgccat gaggcggagc gggctgatcg ggggtgggct cctcgcgacg atgcttctgg
     1681 ttgcctgggt ggccggactg gaggacgacg acctgccggc ccctccatcg gtccgtcgtg
     1741 tgccgcctcg cccgaccgcg cgcagtgtcg gttcgaccgc gctcgacgtg cccccacggc
     1801 gttctgtggc gcctgacatg cgaaacagtt cgggcgatct gtttccggtg gtgagcttcg
     1861 agccgccgcc ccctccgcct ccgccatccc tcaagccggt cgctccgccc ttgccctttc
     1921 gctatgcggg agggctggac gagcgggggg acatgacggc ctttcttgca gacggcgagc
     1981 agatacgcgt cgtgagggcg ggcgagctga tcgacggacg ttatcgggtg gtgctgatca
     2041 gccgtgtccg catcgacttt ctctatcttc cccttaatga accccagagc cttgcgaccg
     2101 ggaatctgcc atgaattgct caatcatccg gcgcgtcctc gcccttgtgg ccgccctgct
     2161 cctggtggtc ggctgcgcgg ccaaccccgc acttgaggag agccgcaagg cctttgccac
     2221 aggccatccg gaggcggccc tgaggagcct gcaggacaag gtgagcgccg atccgaagaa
     2281 tctggaactg cgtgcctact tcctgcgaca gcgcgacacg ctgattctgc ggcaagttct
     2341 tgctgccgat caggcccgca tcgccgggcg cttcgacgag gccgagcgcg ccctcgccgt
     2401 agcccgcccg tttgatcccc gaaatccacg ggtgattgcc gctgcggaag ccattggcgc
     2461 acagaagcag cgcaagctgc agatggagga cgccgagcgg cgcttttcga aaggggattt
     2521 agccggcgcg gaggcggcgg tgcgggcggt gctggccgcc gatccatccc acccggtggc
     2581 cagtgccctg atgcggcgtc ttgacgagcg tgcggcggcc ctggactctg ctccgcttgc
     2641 cctcgccggg cccttctcca agctggtcag cctggagttc cgggatgccc ccttgcgcgg
     2701 ggtgttcgag gccctgtccc gcgcctccgg cctcaacttc gtcatggaca gggatgttcg
     2761 gccggacatc aaggtcaccc tcttcgtgcg ggacaacagc atcgacgatg tgatcaagct
     2821 gctgatggtg acccagcagc ttgaacgcaa gctcctcaac gggaattcgg tgctgatcta
     2881 tccgaatacc ccggtcaagc agaaggacta tcaggagttg gtgacgcgca gcttcttttt
     2941 ggccaatgcc gaccccaaac aggccatggc gatgatcaag caaatggtca agacaaggga
     3001 tgtcttcgta gaggagaagc tgaacctgct cgtaatgaag gacaccccgg ccgccgtgcg
     3061 cctggccgaa cgcctcgttg cagacctgga cctggccgag cccgaagtga tgctggacgt
     3121 ggaagtgctg gaggtcagtc gcaacacgct gctggaactg ggaacgcgct ttcctgacca
     3181 gatcggttac ggccagttac aaccaaccac caccagctcg gtcaccactc cgaccgggac
     3241 cacgctcacc cagagtctcg gcggacagct tctccagggc aatatcaatc tgcgccagac
     3301 gggcgaactt gtgcctttcg tggccaaccc ggggctgctc ctcaatctca aggaccagga
     3361 cggcaattcc aacattctcg ccaatccccg tatccgggtg aagaaccggg acaaggccaa
     3421 gatccacatc ggtgagaagt tgccggtgtt caccacgacc tcaaccgcca acgttggcgt
     3481 gtcgtcgtcc gtcaattacc tggacgtcgg gttgaagctg gaagtcgagc cctcggttca
     3541 ccttgatgac gaggtcgcca tcaaggtgaa cctggaagtg agcagtattg ccaaggaagt
     3601 caccgggccg gccggctcgc tggcctatca ggtgggcacg cgcagtgcga tcacgtccct
     3661 acgtctgcgc gacggtgaaa cccaggtgct tgccgggctc atcagcgacg aggagcgcag
     3721 ttccgccaat cgcctgccgg gccttggcga attgcccggt gtcgggcgcc tgttcagcag
     3781 ccagcgggat gtgggcaaca agaccgagat tgtgctgctg atcacgccca ggatcctgcg
     3841 caatattgcc aaatccggtt ctgccagccc gacgcaggcg gctgggacgg aatcgacggt
     3901 gggggccttg ccgctgttga tcggggctac ccgggcgggc gcgctggggc tcaagtctcc
     3961 ggcgggcgca gcgttgcccc ctgcagtggc ccaggccttg gtggcctcgg ctgcggcacc
     4021 gacgggggtt tccgcgtccg ttgaaggcgt aaggcccgcg ctcaggctgg aggtgccgga
     4081 cgaggtgcat ccgggtgagg agtttgcggt gaccgtgacg cttgaaagcg ttgccgggga
     4141 gggctgctca ctccagatgg ggttcgattc ctccctcgtg gacatcgtcg acgggcagag
     4201 cgagcgtggt gacgtcgtcg tcctgcaact cccggcgggc ccggcgtcca gtctcgcggt
     4261 tcgtttccgg gcccggcccg gcgccgtggg gcgagccggc ttcagtctgt tgtccgcgca
     4321 gggactgacg ccggggcagg ggcaggctgt cttactgccg gcacccggcg gcgtccagat
     4381 cgtgccctga gccatgagcg gccgtggctt taccctgatc gagatgcttg tcaccgtagc
     4441 cgtggtggcg gtactcgctg ccatggccgt gcccgtcggg ctcgtcaccg tccagcgcgc
     4501 gaaggaaggc gaactgcgca cggccctgcg ccagatccgg gaaggcatcg acacctacaa
     4561 gcgctatgtc gacgaagggc gcattgcccg ggcggcggat gcgaacggct atccgccgca
     4621 cctcgaagat cttgtccggg gcgcgcctga cgccaaatcg cccggtgccg cccggatcta
     4681 cgtgatgcgg cgtattccgc gtgacccctt caataccgat gccgcgatcg cggcatcaga
     4741 aacctggggt cggcgcagct atgccagtcc tcccgatgcg cccgaggaag gtagggacgt
     4801 cttcgacgtg tattcccgga gtcccagcat cggactcaac ggaattccct accgggactg
     4861 gtgatgacac ctcggcgacc cattccttgc aagggcttca cgctggtgga attgctggtg
     4921 gtgatggcca tcgttgcgat gctgctgtcc atcgcggcgc cgcgctattt cggtcatctg
     4981 gcacgcgccc gggaggctgc gctggccgaa accctggccg ttacacgtga cgccatcgac
     5041 aagttcaacg gggatgtggg gcgttatccc cgtgatctcg acgaactggt cacgggacgc
     5101 tatctgcggg ccctgccact tgacccgctt accgaacgcc gggacgactg gatattggtt
     5161 gctcctccgg atggcgggga aggcgtatcg gatcttcaca gcagcgcgca gggaaccgct
     5221 cgtgacggac gggccttcaa cgccttatga tgcggcgaaa acatcagggg ggcgccacct
     5281 atctgctcac gctgtttgcg gtggctgcgc tgggtgtggc gcttgcccgt tttggggccg
     5341 tgtggacgac gacgctccag cgcgaacggg aggccgaact gatctttatt ggcggcgagt
     5401 ttgctcgggc gatggccagc tatcgcagtg cgggatccgg tactgagccc gaggcgccgc
     5461 cggaactgga gagcctgctg ctggatcccc gctttcccta tgtccggcgc cacctgcgca
     5521 agctgtacag ggatccgatg acagggaagg cggactggtt gatcgagcga cagggcgggc
     5581 gcatcgttgg cgtgcgcagc cgctccacgc agcatgcgct gcggtgcgcc gctttgcccg
     5641 cttgggtccg caatgttgcg agagttccgg aggatgcgcg ctattccgac tggctctttc
     5701 acccggaaga gaggagtggc gcccgaaatc atggggagac tgcgtcggaa agcggttcct
     5761 tgtggccccc atcaagatga ccgctgagcg gtcgcgccca gatgggtgat cactgcccgc
     5821 ttctgccggc tccgaacccg caatcgaccc gataacaaaa aagccaaccc tcgcggattg
     5881 gcttttctgc ttgcttgtct tggctcctcg acctgggctc gaaccaggga cctacggatt
     5941 aacagtccgg cgctctaccg actgagctat cgaggatctg acggggtggt tacgcatttc
     6001 aggaatgcat caccgaaaag tgttctggct cctcgacctg ggctcgaacc agggacctac
     6061 ggattaacag tccggcgctc taccgactga gctatcgagg aacagaggcg cgcattctag
     6121 ttgcgaagac agtgcacgtc aaggcgatca tgcgcgcgca acacacccgc cccggggatg
     6181 ccggggcagg gtgagggtca ggccttgagg acggcggcga tggcgtcggc gacgtagtcc
     6241 aggttcttgc tgttcagcgc agccaggcag atgcggccgg tgccgacggc gtagatgccg
     6301 aattcggtct tcatgcgctc cacctgggcg gcggagaggc cggtgtagga gaacatgccg
     6361 cgctgcttga tcacgaagga gaagtcctgg tcgaccttgc gggcggcaag ctgttcgacg
     6421 aggccggtgc gcatggcacg gatgcgctcg cgcatgccgg ccagttcggc ttcccactgg
     6481 gcgcgcagtt cgggggtgga cagcaccgcc gcgacgatgg cgccgccgtg gatcggcggg
     6541 ttggagtagt tggtgcggat cacgcgcttg agctgggaca gtacgcgggc ggactcgtcc
     6601 ttgttctggg tgacgatgga cagggcgccg acgcgctcgc cgtacagcga aaagctcttc
     6661 gagaaggagc tggaaacaaa gaaggacagg ccggaggcgg cgaacaggtt gactgccacg
     6721 gcgtccggtt cgatgccgtc ggcgaagccc tggtaggcca tgtcgaggaa aggcaccagg
     6781 ccgcgggtgg cacaggcctt gacgacctca gcccactggg cttcagtgag gtcggcgccg
     6841 gtcgggttgt ggcagcaggc gtgcaggacg atgatggaac cggccggcag gctgttgagc
     6901 gtggcgatca tgccttcggc gttggcgccg cgggtggccg gatcgtaata ggggtagttc
     6961 tcgaccggga agccggcggc ctcgaagagg gcacggtggt tttcccagga cggatcggag
     7021 atgtaaacgg tggcgccggg cacgacgcgc ttgagcaggt cggcaccgat cttgagggcg
     7081 ccggtgccgc cgagggcctg ggcggtgacc acacggccat cggacagcag cgggctggca
     7141 gcgccgaaga gcaggttctg cacggcgttg ttgtaggcgg cagggccttc gatgggctgg
     7201 tagccgcgcg gcggcgccgc ttcgaggcgg gccttttcgg cggtcttgac ggcggccagc
     7261 agggggatct tgccgttgtc gtcgaagtag acgccgacgc cgaggttgac cttggtgttg
     7321 cgggtgtccg cattgaaggc ttcgttcagg ccaaggatcg gatcgcgggg ggccatgtcc
     7381 accgcggcga agatcgaaga gctcatggat tctccagttg ggcgaagggt gcgcgctgct
     7441 gcggggcgcg cgctgggcga taatgcaggg ctgccggtgt ttcccggcgg gcacggcatt
     7501 ctgactcaaa actgacattt tatcatggcc gatcaaaccg atagcgggcg cgtggtgtgc
     7561 ttcgagggca gccccttccg tcttcaccag cccttcccgc cggccggcga ccagcccgag
     7621 gcgatccgcc tgctggcgga gggcattggc gatggcctga tgttccagac cctgctgggt
     7681 gtgaccgggt cgggcaagac ttacaccatg gccaacgtga tcgcccgctg cggccgcccg
     7741 gcgctggtgc tggcccacaa caagacgctg gccgcgcagt tgtatgcgga gttccgcgag
     7801 ttcttcccgg agaacgcggt ggagtacttc gtgagctatt acgactacta ccagccggaa
     7861 gcctacgtgc cgtcccgcga cctcttcatc gagaaggact cgtccatcaa cgagcacatc
     7921 gagcagatgc gcctgtccgc caccaagagc ctgatggagc ggcgcgacgt ggtgatcgtg
     7981 gcttccgtgt cgtgcatcta cgggatcggc gatccggtgg attaccacgc catggtgcta
     8041 cacctgcgtg aaggcgagcg catggcccac cgggatctgg tgcagcgcct ggtggcaatg
     8101 cagtacaccc gctccgacgt ggatttccgg cgcggcacct tccgtgtgcg gggcgacgtg
     8161 atcgatatct ttccggcgga aaacgccgag tacgccctgc gtgtcgagat gttcgacgac
     8221 gagatcgagc acctgaccct gttcgaccct cttaccggac acctcaagca gaagatcgtg
     8281 cgcttcacgg tgtatccgtc gagtcactac gtgacgccgc gggctacggt gttgcgggcc
     8341 attgaggcca tcaaggacga gctgcgcaca cgcatccaga ccttccagac cggcggcaag
     8401 ctggtggagg cccagcgcat cgagcagcgc acccgcttcg acctggagat gctcaacgag
     8461 ctgggcttct gcaagggcat cgagaactac tcgcgacacc tctccggccg tgccgcgggc
     8521 gagccgccgc cgacgctgat cgactacctc ccggcggacg ggctgatgtt catcgacgaa
     8581 tcccacgtca ccatcggcca ggtgggcgcc atgtacaagg gcgatcgttc gcgcaaggaa
     8641 aacctggtgg agtacggctt ccgcctgccg tcggcgctgg acaaccggcc gctgaagttc
     8701 gaggagttcg agcgtctgct accccagacc gtgttcatct cggcgacgcc ggcggcttac
     8761 gaagcggcac accagggcca ggtcgtggag caggtggtgc ggccgaccgg gctggtggat
     8821 ccggtgatcc acgtgcggcc ggcatctacc caggtggacg atctgctcgg cgagatcaag
     8881 ctgcgggtcg ccgtcgagga gcgcgtactg gtgacggtgc tcaccaagcg gctggcggag
     8941 gatctcacgg aatacctggc agagaacggc atccgcgtgc gctacctgca ctcggacatc
     9001 gacacggtgg agcgcgtcga gatcatccgc gatctgcgcc tcgggcactt cgacgtgctg
     9061 gtgggcatca acctgctgcg ggaggggctg gacattcccg aagtgtccct ggtggcaatc
     9121 ctcgatgcgg acaaggaggg cttcctgcgc tcggagcggt ccctgatcca gaccattggc
     9181 cgcgccgcgc gtcatctgca cggcacggcc atcctctacg ccgaccggat cacggattcc
     9241 atgcgcgccg ccatgggcga gaccgagcgc cgtcgcgcaa gacagctggc tttcaatgcc
     9301 gagaacggca ttacaccgcg cagcgtgaca aagcgcataa aagacatcat cgacggcgta
     9361 tatgatgcga aggacgagga tgctgctgtc gtggctgccg agcccgctgc ggagtatgcc
     9421 gcgatggacg agaaggccct ggcccggacg atccggcaac tcgagaagga catgctcgaa
     9481 cacgctcgca atctggattt cgagaaggcc gccgcggtac gggaccagtt gttccggctg
     9541 cgcaggcagg ccttcggtgc cgaaacccac gatacgagcc tccctccgga cgcgaaccgc
     9601 tgatcgattg gaagattttg gtgctgaccc ttgtacggac ggcccgccgc ctgcttaaat
     9661 taccagcagg aggagggacg cccataatga aaatattatt tgtctgcacc ggcaatatct
     9721 gccggtcgcc gacggcggag ggggttgcgc gccgctggtt ggccatgacg ggtctggagg
     9781 gcatcgtcgg agtcgattcg gcgggcatcc agggttacca cgccggtgaa gcgccggatc
     9841 cacgagccca gaccgcagca atgcggcgtg gctacgatct gtcgcggctg cgcgcacgca
     9901 aggtgctgct caaggacttc gccgaattcg acctgatcct ggccatggat cgggatcatt
     9961 tccactatct gcgggacatg gcgccggaag gctgcaagca caaggtgcgc atgttcatga
    10021 gcttcgccca gacccatcgc cgcgatgacg tgcccgatcc ttactatggc gggatcaccg
    10081 gattcgacgc ggtgctggac atgtgcgagg acgcggtgca gggactgatc gacgatatcc
    10141 gtccggacgt ggagcgccgt caccgctcgg tggtgtagac gcgcagcgtg cgtcgagcgc
    10201 cggaagcggc gcgcagtccg ccacgcgccc atgaaaaagg ccaacccctg cgggttggcc
    10261 tttttgttgg cgggatcgcc ggtccttact tggccccggt ttccacctga acgagcggct
    10321 cctccgccac cggtgccacc gcgcgacgac ggcgtcccaa aggctgggcc ggttcgaccg
    10381 gcgccacggg tgcggcagcg tcacgacggg tctcgatgag gatcaggccg ctgtccttga
    10441 gggtctcgtc cagcgtggcg accgcttctt cgaccacggg cagggcctcg acgacgggtg
    10501 ccgctgcagg cgcctccagg ggcacttcaa cgggcgtcgc gaagaaggat ggggccgccg
    10561 cgggcgcgtc agccacgccg ggctcgggca caggcgccgt ctcgggttcg gtcggcgttt
    10621 cctggggcac ggtttcctcc acggctgcga cgggggcgac cgcgtccacc acgggcgctt
    10681 cggccgtcac cggggccgcg tcggacgcca cctctgcggc cggctcgaac acgggttcga
    10741 ccgatgcagc cgcggcactc acggtttcca cttgctgcgg cacgacggcc tcgaccacgg
    10801 ccggctcgat ggcaggcgtg tcggtcacgg ccggggtgtc ggcttcagcc gcgggcacgg
    10861 cttcggcgac ctccgctgcg gcggtgccga tctcgagctc accctgggca gcgctctcgc
    10921 tggtgccgga ctcctcgttg cgacgaccgc cgcgacggcc gcggcgacgg cgattgcgct
    10981 cgccattgcc ttcgccttca ctggtcggcg caggcatcgc ggcgtccacg acggcgacac
    11041 tggcaacggc ttcaagcttg ttgtcagcat cgctgaccgc ggcttccgtc tcgacggcgc
    11101 gcggtgcctg ggcgctattg cggttgcggc cgcggccttc gccgcgctcg ctgcgttcac
    11161 cccgctcggc gcgttccgga cgctcgcccc gttcgccgcg ctcctggcgt tcgccgcgct
    11221 ggcgctgcgg cttttcgcgg gtttcggcgg cggcttcggg tgcgcgggtg tcgcggggct
    11281 cgttacgctc gctccgctcg gaacgttcag cgcgttcgcc gcgctcttcc cgctggccgt
    11341 cgcgacggtt gcggccatcc cggcggttgc ggtcgttgcg ggagcgggat tcgcgacggg
    11401 caggttccgg ctgggcgacg accggcgcgg gctccggctg cttctcgccg cggaagaagg
    11461 cgaagatgcg ggcgagcagg ccgggctggc tttcgggcac gggggcaggg gccggtgcgg
    11521 gcttggcttc cacggtgggg gccggctggt ccggagtgat gcccttgacg gcggcttcct
    11581 ggcgggccgg acgatcattg ctggccgacg gcggggtgta ggtcacgtca gacggctggg
    11641 tcgccatctt gtagctgggc aggacgtgct cttcctggtt gagctggtcg tgacgcaggc
    11701 gcacgatttc gtgctgcggg gtttccaggt gacggttggg gatgaggatg aggttgacct
    11761 tgtggcgcac ctcgatcttg gcgatgtcgt cgcgcttttc gttgaggagg aaggtcgcca
    11821 catcgacggg gacctggcag tgcagggcgc cggtgttgtc cttcatggcc tcttcttcga
    11881 ggatgcgcag gatgtgcagg gccgaggatt cggtgctgcg gatgtggccg gtgccgttgc
    11941 agcgcgggca ggggatgtag ctggtttcgg cgagggcggg gcgcaggcgc tggcgcgaca
    12001 gttcgagcag gccgaagcgg ctgatcttgc cggtctggac gcgggcgcgg tcgtagcgca
    12061 gggcgtcgcg caggcggttc tcgacctcgc gctggttctt ctgggactcc atgtcgatga
    12121 agtcgatgac gatgaggccg cccaggtcgc gcagacgcag ctggcgggcg acttcgtcgg
    12181 ccgcttcgca gttggtcttg aaggcggttt cctcgatgtc ggcgccgcgg gtggcgcggc
    12241 cggagttcac gtccacggac accagggctt cggtgtggtc gatgacgatg gcgccgccgg
    12301 agggcaggtt gaccgtgcgc gagtaggccg actcgatctg gtgctcgatc tggaagcggg
    12361 agaacagggg cacgtcgtcg tggtagcgct tgacccggtt cacgttggtg ggcatcacgt
    12421 gggccatgaa ctggcgggcc tgctcgtaca cgtcgtcggt gtcgatgagg atctcgccga
    12481 tgtccggctg gaagtagtcc cggatggcgc ggatgacgag gctgccttcc tgatagatga
    12541 ggaaggcgcc ggcctgggcc tgggcggcgc cgtcgatggc gcgccacagc tgcagcaggt
    12601 agttgaggtc ccactggagc tcttcggcgc tgcggccgat ggccgcggtg cgggcgatga
    12661 ggctcatgcc gccgggcact tcgagctggt ccatgacgtc gcgcagctcg gcgcgctcgt
    12721 ccccttccac gcgccgcgac acaccgccgc cgcggggatt gttgggcatc agcacgaggt
    12781 agcggccggc cagggagacg aaggtggtga gggcagcgcc cttgttgcca cgctcgtcct
    12841 tctcgacctg gacgatcagc tcctggcctt ccttgagggc ttccttgatg gaggcgcggc
    12901 tggcttcgac acccggctgg aagtaggcgc gggagatttc cttgaagggc aggaatccgt
    12961 gacgctcggc gccgtagtcg acgaacgcgg cttcgaggct gggctcgatg cgcgtgatga
    13021 cggccttgta gatgttgctt ttacgttgtt ccttggcggc agattcaatg tcaaggtcaa
    13081 tgagtttctg accatcgaca atggcaacgc ggagttcctc tgcctgcgtt gcattgaaga
    13141 gcatgcgctt cattcgtctt tttctcccgc gagacacacg ggcaggacga accgcgccac
    13201 cgcctaggag cggtgactta gtttggcgtg atcgcgacca tccttcattg aggggggaat
    13261 caaactaacg ggggcggcgg aattcgtctg agtcgcaccc tgaaccgcgt tctgcgccgg
    13321 cttcagggtc gtcaatctgc gtgtgcgcct gcgcgcaata ggtgcaagtt tattgtcgaa
    13381 tacagtacca tcgccctctt ttgtctggcg acggaccttc ctgtgacttt ttcgggagca
    13441 gggaaggcgc agtgggcgct tccgcacatc gctctggaaa ggggcgcggg ccggcgttga
    13501 atttcgatac tccgtgccag acggagtgcg cgcatcaacg aatgtcgtgg gcaggcgcga
    13561 cggttccgga ccgaaaaagc cgctagacag tatatttcaa aacgatggcc ttgagtaaag
    13621 cccccgcagt gagtcgcgta atggtcgatg agtccgccgc cggccagcgt atcgacaatt
    13681 ttctgatccg ccattgcaag ggtgtgccga agagtcacgt ctatcgcatc ctgcgcagtg
    13741 gcgaggtgcg cgtgaatagc cgccgggtgg cacagaccta tcgcctgcag tccggtgacg
    13801 aggtgcgcat cccgcccctg cgtctggccg agcgggagga cagctcgccg gtgccggtgg
    13861 gcaaggcttt cgcgatcgcc tatgaggacg aggcgctgct ggtgctggac aagccggccg
    13921 gcctggccgt gcatgggggc agcggcgtga gcttcggtgt catcgaacag ctgcgccggc
    13981 agcggccgga ggcgcgcttt ctggagctgg tccatcgtct cgaccgggaa acgtccggac
    14041 tgctcatcgt ggccaagaag cgcagcgcgc tcacggtgct ccacgacatg atgcgcgacg
    14101 ggcgggtgca gaagcgctac ctgaccctcg ttgcggggcg ctggatgaat ccgctgcaac
    14161 atgtgaaggc gccgctgctc aaatatctga ccgaaggcgg cgagcggcgc gtcagtgtgc
    14221 acccggacgg caagccggcc cacagcatcg tgcgtctgca caggcgctgg cagagctgca
    14281 gcctggtgga ggtggagctg aagaccgggc gtacccacca gatccgcgta cacctgacgc
    14341 accagggctt tcctttgtac ggggacgaca agtacggtga cttcgctctc aacaagcagc
    14401 tcgagaaggc cggactgaag cgcatgttcc tgcacgcggc gcatctggcc ttccgccatc
    14461 cgctgaccga tgcgccgatc gaactttcgg cgccgctgcc ggacgaactt gcggccttca
    14521 ttgtggccgt ggaacaggct gaggagcggg actatggtta agcgattcga tctgatcgtc
    14581 tttgactggg acgggacgct gatggattcg gcggccgcca tcgtccgcgc catccagtcg
    14641 gccagcaccg atctgggcct gccggtgccc gacgatgcgc gggcccgtca tgtgatcgga
    14701 ctggggctgg tggacgccct gcgccatgcg gtgccggacc tggccgaaca cgactacccg
    14761 cgcatggtgg agcgctatcg ccaccattac ctgtccggcg atcacgaact gaccctcttt
    14821 gacggcacct ttgacatggt ggaagccctt catgggcgcg gccatctgct ggcggtggcg
    14881 accggcaaga gccgcaaggg tctggaacgg gcgctggggt tctcgggtct cggtcgcttc
    14941 tttcacgcca cccgctgcgc agacgagtgc ttctccaagc cccatccgca gatgctggaa
    15001 gagctgctgg acgagctggg cgtgatgccc gacgcctgcc tgatgatcgg cgacacgacc
    15061 cacgatctgc agatggcgcg caatgccggc gtggcggcgc tggcggtcag cttcggcgcc
    15121 catccggtcg aacagctcga ggcagaggcg ccgctggcct gcctccattc accggtggcc
    15181 ttgcgcgact ggctggagcg gcacgcgtga tctgtcggtc tgacgaggtc ctggaagggg
    15241 gcgacggcat ccgcttcacg gttcaggtgt gtggtgaggc cgttgcagcc tttttggtgc
    15301 gctacggcgg acgggtgtat gcctaccgca acagttgcgc ccatgtgccg gtggaactgg
    15361 actggcaacc ggggagattc ttcgatactg gtgggacgta tctgatctgt gccacccacg
    15421 gggcgctgta cgaaccggaa tccggctact gcgtggccgg tccgtgccgg ggccgtcgcc
    15481 tggttccggt ggcggtggag gagcgggacg gcggcgtgtt tctgaagggc gtactgggcg
    15541 cctgagtgct cagccggcca ggatcagaaa gacggtcggg tgccgggcca gttctggcgc
    15601 ggcgctcttt ctccaggccg ccaccgtgcg ggtggcgatc cattcgctgt cgaggctcag
    15661 gtcgcgggcc acgcacaggc gcgtggtagg tgcgcagacg gtgcgcaggg cctcgaacat
    15721 gcgttcgttg cgatagggcg tctcgatgaa cagctgggtg cgctgctggc ggccggattc
    15781 ggcttccagt tcgcggatgc gtcggttgcg ggcgtcctcg tccaccggaa ggtagccgtg
    15841 gaaggcgaag ctctggccgt tgaggcccga ggccatgagg gccagcagca gggacgacgg
    15901 ccccaccagc ggtaccacgc gcacgccggc ttcatgggcg cggcgcacca gcagggcccc
    15961 cggatcggcg acagccgggc agcccgcttc cgacatcagg ccgccgtcgg tgccggcgag
    16021 aatgggggcg aggagcgcgt cgagatcggc cgggttgggc ttgtcgggca gctcgcggat
    16081 gtccagctcg cgcagcgggc gggggtgctc caggcgcttg agttcggcgc gggcggactt
    16141 ggcgttctcg accacgaaat agtcgagggc gcaggcttgc gcggccacgc cggcgggtgt
    16201 ggtgagggcg gtgggggcgg gccccaggct caccggcacg agatacagcg tgccggccat
    16261 cagggcaggg cgatgccttc ggcacgcagc atgcgggcga gggcgatgag cggcaggccc
    16321 accagggcgt tggggtcgtc accggtgagc gcgtccagca aggtgatgcc gaggccctcg
    16381 gacttggcgc tgcctgcgca gtcgagggga cgttcacgct cgacgtagcg gacgatctcg
    16441 tcctcgctca gggcgcggaa gcgcacctcg gtcggcacca catccacctg cacggcgcca
    16501 ttgcgggtgt tgagcagcgc cagggcggtg tggaagacga ccgtctgccc gctcatggtc
    16561 ttgagttgcg cgacggcgcg ttcgacgcag cccggcttgc cgaaacggtg ttcgccgagg
    16621 gccgccacct ggtcgctgcc gatgatcagt gcatcgggaa actgtgccgc gaccgcacgg
    16681 gccttgccga cggcgagccg ctcggcggtg gcgcgggggc tctcgtcggg cagtggcgat
    16741 tcgtcgatgt ccggtcgggc cacgtcgaag ggcaggccga agcgcagcag gagttcccgg
    16801 cggtaggccg aggtggaggc gaggacgagt ttcatcgggg cggggcccgg agtgccggaa
    16861 cgtgaacgca tcgaggcaca ccgtgcgaac tgggttttga cacgggcagc ccgaaataat
    16921 atcattccgc gcttatgtcg caagaaggct tcgtgatcga tcttcgggcg ctcgccaggg
    16981 atggccggca cgtcgagggt gaaattccgg tggcccggct cgaacgactc gccgattcgg
    17041 tgagcgatcc ggtgggttca gtgtggtttc gctttgacgg ggtgcgggac gacgatggca
    17101 agctgttcgc cgatctgatg atccggactg agttgacgct gcagtgtcag cgttgtctcg
    17161 atgcgctgcg cctgccctgc gtgatccata accgtctgct gctcctgcgc cctggcgagg
    17221 agattccgga agacgatctg gaaaacgatg aggtggatgc gctggaggtc gagccgcgga
    17281 ccgacgtgct ggcgctggtg gaggatgaac tgctcctcgc gctgccgctg gtgccccgtc
    17341 acgaggcttg cgaagcgccg gtgaaagccg gcggcgatga agagatttcc cccttcgccg
    17401 tgttgcggaa tctgcgcggc aaggcttaag tattcctcaa ggagtgtttc atggctgtcc
    17461 aacagaacaa gaagtccccg tccaagcgcg gtatgcaccg ttcccacgat ttcctggtgg
    17521 ctcccccgct ggccgtcgag ccgaccaccg gtgaggtgca tctgcgccac cacgtgtcgc
    17581 cttccggcgt ctatcgtggc aagaaggttg ccaaggccaa gggcgaataa tctccccggt
    17641 ccgaaagcgg cgcggcaccc ctgggtgaac gcgccgtttt tgcgagtgaa cctcttgccc
    17701 gacagcgaat gacagtcacc ctggcgatcg actgcatggg gggcgatcac ggcccctccg
    17761 tcaccgttcc ggcggccttg cgctttctcg ataccgatcc ggatgtccgg gtggtgctgg
    17821 tggggcgtgc cgaagccgta gaacagcaac tcggtgtggc ccaggtgcgc tacggcgagc
    17881 gcctgagcat ccgccatgcc accgaagtgg tcggcatgga cgagccgccg gccctcgcga
    17941 tgcgcaacaa aaaggactcg tccatgcgcg tggcggtgga tctggtcaaa tccggagacg
    18001 cccaggcggc cgtgtcggcc ggcaatactg gcgcgctgat ggccatctcc cgcttcgtgc
    18061 tgaagacgct gccgggtatc gaccggccgg cgatctgcag cacgctgccg accctgcgtg
    18121 ggcagacgca tgtcctcgac ctgggtgcca atgtggattg ctcgcccgcg catctgctcc
    18181 agttcggcat catgggtgcc atgctggtgg ctgcggtcga gcacaatgag cgtccgagcg
    18241 tcggtctcct caacatcggt gaggaagaca tcaagggtaa cgagacggtc aagcaggccg
    18301 gcgagctgtt gcgccatagc ggactcaatt tctatggcaa cgtggaaggc aacgacatct
    18361 acaagggcac cgtggatgta gtggtctgcg acggtttcgt cggcaacgtg gccctcaaga
    18421 cctccgaagg cctggcccag atgctggcca ccttcctgcg cgagcagttc tcccgcaact
    18481 gggtgaccaa gctggcggcc gtcttcgcca tgtcggccct caagggcttc aagcacaagg
    18541 tggattcgcg ccgctacaac ggcgcggccc ttctgggtct gcgtggagtg gtggtgaaga
    18601 gccatggcgc cgccgacgtc tttgcctttg agcaggctat ccggcgtgca gtggaagcgg
    18661 cgcgcaacaa tctgatcgag cgcatcagcg cacgcatgga acgggagagg cccgcggcat
    18721 gatctatgca cgagtggccg gcacgggcag ctatctgccc ggcgcgccgg tgaccaacga
    18781 cgacctggtg gcccgcggca ttgacacatc cgatgcgtgg atccaggagc gcaccggcat
    18841 ccgcagccgc cacctggctt cgcccgaagt cacctccagc cagttggccc aggaggccag
    18901 cctgcgggcc ctggaggcgg ccgcctgtac gcccaacgac atcgatctgg tgatcgtcgc
    18961 cacctccacg cctgattaca tctttccgag tactgccacg ctcctgcagg cccggctggg
    19021 catgagcaac ggtgcgccgg cgtttgatgt ccaggctgtc tgtgccggtt tcgccttcgc
    19081 catcgccacg gccgaaaagt tcatccgttc gggttcgcac aagcgtgccc tggtggtcgg
    19141 tgccgaggtc ttctcgcgca tcctcgattg gaacgatcgc ggcacctgcg tgctcttcgg
    19201 tgacggtgcc ggtgcggtgg tgctcgaagc gtccgagcga ccggggatcc tggccaccgc
    19261 gctgcacgcc gatggagcgc agaatcagat cctgtgcgtg ccggggcaga tcagccgggg
    19321 tcaggtgacc ggcgatcctt tcctgcggat ggatggtcag gcagtgttca agtttgctgt
    19381 caaggtgttg tccgacgtgg ccaaggaagt ggccgagctg gggggcgtgc ccctcgacgc
    19441 ggtggattgg ctgattccgc atcaggccaa catccgcatc atccaggcca ccggcaagcg
    19501 cctgggcgtc gcgccggagc gggtgatcgc caccgtgggc gagcacggca acacttcggc
    19561 ggcctcgatt ccgctggccc tcgatgcggc ggttcgcgac gggcgcatcc ggcgcggcca
    19621 gaaggtgatc ctggaaggca tcggcggcgg cttcgcgtgg ggcgcgctgc tcctcgaata
    19681 ctgagttctc gatacggcat cgaagggtcc acaacaatga atttcgcgtt tgtttttcct
    19741 ggtcaggggt cccagtccgt cgggatgatg gccgcctacg gcgattccgc ggtgatccgt
    19801 gacaccttcg ccgaagcctc cgcagcgctc ggtgaagacc tctggcagat ggtggcggat
    19861 ggccccgccg aaaccttggc gctgaccgtc aacacccagc cgttgatgct gacggccggc
    19921 attgccgtct atcgcgaatg gatcgcccgc ggtggccgta cgccggcgct ggtggccggt
    19981 cacagcctgg gtgagtactc ggcgctggtg gcggccggtg cactgggctt tcaggacgcg
    20041 gtcaagctgg tgcgtctgcg cgcccaggcc atgcaggaag ccgtaccggc cggcgaaggt
    20101 gccatggcgg cgctgatggg cctcgatcag cctgctgcgg ccgaagcctg cgccgaagcg
    20161 gcccagggtg aagtcgtctc gccggccaac ctcaactcgc cggggcagat cgtcatcgcc
    20221 ggtgcccgtg ccgcggtgga gcgcgccatt gcggtcgcca agaccaaggg cgccaagcgc
    20281 ggcgtgctgc tgccggtgag cgcgcccttc cactgcgcac tgatgaagcc ggcggccgag
    20341 cggctcgccg tggcgctggc cgatatcgtc gtggcggcgc cggccattcc ggtgctcaac
    20401 aacgtggacg tggcggtcga gaccgatccg gcccgcatca aggacgcgtt ggtgcgccag
    20461 gcctattcgc cggtgcgctg gatcgagtcc gtccaggcga ttgcggggcg cggcctgacg
    20521 cacgtctacg agtgcggtcc cggcaaggtg ctggcgggca tgaccaagcg tattgagggc
    20581 agcctcaccg gcggcgcgat cgccgacgcg gccagccttg acgagattct ccagacgacg
    20641 ggggcggcat gagtcaggtt ttgcagggac aggtggcgct ggtgaccggc gcctcgcggg
    20701 gcattggacg ggcgatcgcc ctcgaactgg ggcgcctcgg cgcgacggtg atcggcacgg
    20761 cgacctccga agcgggcgct gccgacatcc aggccgggct cgacgcggct ggggtcagcg
    20821 gccgcggcat gatgttgaac gtcaccgacg ccgcggcctg cgaagcggcg ctggccgaga
    20881 tcgaaaaggc cttcggtccg gtgctggtgc tggtgaacaa cgccggcatc acccgcgaca
    20941 acctgtccat gcgcatgaag gacacggaat gggacgcggt gatcgacacc aacctgaagg
    21001 cggtgtttcg catgtcgcgg ctggtcacca agggcatgat gaaggcccgt aacggtcgca
    21061 tcatcaacat cacctcggtg gtgggcagct cgggcaatcc cggccaggcc aactatgcgg
    21121 cggccaaggc cggcgtagca ggcatgaccc gttccctcgc ccaggaactg ggcagtcgca
    21181 acatcacggt caattgcgtg gcgccggggt tcatcgacac cgacatgacc aaggaactgc
    21241 ccgaagatca gaaaaaggcg ctgcagacga agatcgccct cggtcgtctg ggcaagcccg
    21301 aagaaatcgc ggcggtcgtg ggcttcctcg cctcgcccgc cgctgcctac gtgacggggg
    21361 ccaccctgca cgtgaatggc ggcatgtaca tggcgtgatc ctcgccgcgg tggcgagggt
    21421 gcgcattttt tggcagaatg cgggggtttt tgaattcacc cgcacaatca gggaaggagt
    21481 tacacatgtc cgatatcgaa cagcgcgtca agaagattgt tgccgagcaa ctgggcgtca
    21541 acgaagccga agtgaagaac gaatcctcct tcgtggatga tctgggtgcc gactctctcg
    21601 acaccgtcga gctggtgatg gccctcgaag aggaattcga gtgcgagatc cccgacgagg
    21661 aagccgaaaa gatcaccacc gtgcagcagg ccatcgatta cgtgtccgct cacctgaaca
    21721 agtaattcct tcgatccgga gttcgtcttg gccgctcgca gagtcgtcat caccgggctg
    21781 ggagtcatct ccccggtcgg taacaccgtg tccgaagcct gggacagcat cctcaacggc
    21841 cgttccggga tcggccccgt cacccgattc gacgcatccg ccctgtcggc ccggatcgcc
    21901 ggggaggtca aggggttcga tgtcggcgcg tacatttccg ccaaggaagc gcgccggatg
    21961 gacactttca tccatttcgg gctcgctgcg gggattcagg caattcgcga ttccggaatc
    22021 gaagtcaccg acgccaatgc agatcgcatc ggggtgaaca tcggctccgg catcggcggg
    22081 ctgccgatga tcgaagggac gcacaccgac tatgtcgctg gtggcccgcg caagatctcg
    22141 ccgttcttca ttccgggcac gatcatcaac atgatctctg gcaacctgtc catcatgttc
    22201 ggtctgaagg gaccgaacct ggcggtggtg acggcctgta ccaccgggct gcacgccatt
    22261 ggcagcagca tgcgcatgat ccagtatggg gatgcggatg tgatggtctg cggcggagcc
    22321 gaatcgacgg tgtgccccct ggcgatgggt ggcttcgcgt cggcgaaggc cctgtccacc
    22381 cgcaacgacg atcctgcgac ggcgagccgg ccgtgggaca aggaccggga cggattcgtg
    22441 ctgggagaag gcgccggtgt gatggtgctc gaggaatacg agcatgccgt gaagcgcggc
    22501 gcccgcatct atgcggagct cgccggcttc gggatgagcg gtgatgcctt ccacatgacc
    22561 gcaccggaca ccgacggccc ccgtcggagc atgctcaacg cgctgcggga cgccggcgtg
    22621 aatcctgatc aggtgcagta tctcaacgcc cacgggacct cgacgccgct gggtgacaag
    22681 aatgagaccg aggccatcaa gcaggccttc ggcgtggagg tggctaagac gctggtggtc
    22741 aattccacca agtccatgac gggtcacctg cttggtggcg cgggtggtct tgagtccgta
    22801 ctgactgccc tggcggttca tcaccaggtt tcgccgccga cgatcaacat cttcgagcag
    22861 gatccggagt gcgatctgga ttactgtgcc aatgtcgccc gtcccatgaa gattgatgtg
    22921 gcgctgaaga ataatttcgg ctttggcgga accaacggtt cgctggtgtt ccgccgggtt
    22981 tgaccgggtg cgtggtcccg ggggttcggc tccgtgatca atgtgcttga attcgggccg
    23041 tcgcggctgc gcgtcatctt gctggtgaca ctgcacgccg cggcggcttt ttcatgtgtc
    23101 gccggcctgg catcgccgtg gatcaccgca ccggtcgggc tggtgatcgc agcatcgctt
    23161 tatcgggaag tgaagcaggc gctggggcag tccggtcgcc tggaactgca tgcggacgga
    23221 cgtctggcgt ggcagttcag cggaatggcg gcacaggccg gacgcgtgcg cgcggagtcc
    23281 gtcgcgcttc atcgttcgat ctggctgatc tgggacgacg atgggcgacg tggcgcccgc
    23341 tggctgttgc cggaccagat gtccacgcag gactggcgcg ccttgcaggt atggttgcgt
    23401 ctgcgcgtga ccaagacgtt tgcggagccg gactcggacg tcgcctgaat gatcctgcgc
    23461 gtccgcgcct tcagctgcgg cgtggtggac ggaggacagt ctcccgtgcc ttgggctgga
    23521 gctgcggata atcacggctg aagtgcaggc cgcggctttc cttgcggcgc agggcgctgc
    23581 gaacgatcag atcggcggtc accaccaggt tgcgcagttc gatgaggtcg ttggtcaccc
    23641 ggaagttgct gtagaactcg tcgatctccc gggccagcag acggatccgg tgctgagcgc
    23701 gctgcagccg cttggtggtg cggacaatcc cgacgtaatc ccacatgaag cggcgcagtt
    23761 cgtcccagtt gtgggagatc acgatggcct cgtcagcgtc ggtgacgcgg ctctcgtccc
    23821 atgtcggtag atccgcgggg gcgtggcgaa gatggctgcg gatgtcctgg gcggcggcct
    23881 cggcaaatac caggcattcc agcagggaat tgctggccag ccgattggca ccgtgcaggc
    23941 cggtgcaggc ggtctcgcca atcgcataga gaccggggac gtccgtgcgg gcatgcaggt
    24001 cggtgacgac gccgccgcag gtgtagtggg cggcgggcac caccgggatc ggttgctggg
    24061 tgatgtcgat gcccagttcg aggcagcgtg cgtggatgtt ggggaagtgt tccgcgatga
    24121 attcggccgg cttgtggctg atgtcgagga agacgcagtc caggccgtgc ttcttcatct
    24181 cgaagtcgat ggcgcgggcc accacgtcac ggggcgcgag ctcttcgcgg gtatcgtgct
    24241 cgggcatgaa gcggctgccg tccgggcgcc gcagcaggcc gccctcgcca cgcacggctt
    24301 cggaaatcag gaaggacttg gcctgcgggt ggtagaggca ggtggggtgg aactggatga
    24361 actccatgtt cgccacccgg cagccggccc gccaggccat ggcgatcccg tcgcccgtgg
    24421 ccgtgtcggg attggtggtg tacagataga ccttgcccgc accccccgtc gccagcaccg
    24481 tgtggccggc cgcgaaggtg cgtacctcgt cgtgcgcgat atccagcacg taggcgccga
    24541 ggcagccttc ctccagacgc ccgatcttgg acccgacgat caggtcgacg gtgatgtgct
    24601 gttcgaagat ggcgatgttg ggatgggccc ggacctgctc ggtgagcgtg gcctgtacgg
    24661 cggcaccggt ggcgtcggcc acgtggatca cgcggcgctg accatgcccg ccttcgcggg
    24721 tgaggtggta gccaatgggt gactgggcat ccgcggtgaa gggcacaccc cgctcgatca
    24781 gccactggat ggcacgggcg ccgttttcga cgacgaagcg cgaggagcct gggttgcaca
    24841 agccggcgcc ggccacaaag gtgtcggtca ggtgggactc aatgctgtcc gcgggatcga
    24901 gcacggcagc gatgccgcct tgtgcccagg cgctggcgct gtcttcgaga tgccgtttgg
    24961 tgacgagggc gaccttgtgg gtgtcggcga ggcgcagcgc cagggattgg ccggcaagac
    25021 cgctgccgag gatcagtacg tcgaaaacgc tcacttctgg gggtgcctcg gagggctcga
    25081 gatcgaaggg gcgactatag cacgtgaaat tgcccctgct cagggcgggg tcggactgaa
    25141 cttacgccgt ggcggcgggt ctagcgagtg ctttggcgga gatttggagg ctcacggtat
    25201 actgggggtc ctgcgaccgt tttgggttgc gatgttccga ttccagtatt ccgaccatga
    25261 gcgatcgcga aattgaccag cagttggtcg agcgtgtaca gcgcggcgac aagcaggcgt
    25321 tcggcctact tgtggcgaag taccagcgta agctcgcccg cctgctgtcc cgtctgatcc
    25381 gagaccctgc cgaagtggag gacgtcgccc aggagacgtt catcaaggcc tatcgggccc
    25441 ttccgtcctt tcggggggag agtgcttttt acacctggct gtaccgcatc gggatcaaca
    25501 ccgccaagaa cttcctcgtg tcgcaggggc gcagggcacc cacgacgact gaattcgatt
    25561 cggaggaagc ggaaaccttc gaggacggcg atcagttgcg cgacgtggat acacccgagc
    25621 ggctgttgat gaccaagcag atcggggaga cggtaaacct tgcgatggag gccctgccgg
    25681 aggaactgag ggcggccatc gtgttgcggg agatcgaagg gctgagctac gaagagattg
    25741 cgacgatcat ggggtgtccc atcggcaccg tgcggtcgcg catcttccga gcacgcgagg
    25801 ccatttccga aaagctcaag ccgttgctcg acacggctcc gaacaagagg tggtgagaag
    25861 atgaaagaac gtgtttctgc cctgctcgac ggtgcgctgg acgaggaatc gacggtgcgc
    25921 atgctcgatc atctgaagcg ggatggggca ttgcgacggg actgggagca tttttgcctg
    25981 gtgggcgatg ccctgcgggg cgatgaaccg ctggccctgg acgtcactcg cagtgtgatg
    26041 ctgcgtctcg aagacgagcc caccgtactc gcaccggcgg ttcggcggga agggagcggc
    26101 ttcttccggc atctcatgcc tctcgctgcc tcgctgatgg gggtggcggc ggtaggctgg
    26161 gttgccggca tgttcagcgg tggcggtgac gcgctgcact ttgccaaggt gaacaaatct
    26221 cccgcgctga tggccagtgt gacgcctgcg acgtccggac agtcgatggt gatgccgacc
    26281 tccgtcgaga attcgaaccg ggaatacctc ttcgctcacc agtccctggc gccctcggcg
    26341 gcgatgccgg gcgtcgcgct gtatgtcagg acggtggcgg acaccgccat gcccggtcag
    26401 cgatgagtcg cttccggcat tgcctgctgg tcctgctggc ctgttgcgga actgcccgcg
    26461 ccgaggcgcc cggtgacccc ctgagctggc tcgggcgtat tgccggtgcc gcccatcggt
    26521 tgaattattc cgggacgttc acctatcagc acggcaagac ggtcgagacc tcccggattg
    26581 cccacctggt ggatggcagc ggcgagttcg agcggctcga agcgcttgac ggcagtccgc
    26641 gggaagtggt tcgtagcaac gatcaggtcc agtgctacct gccggatcag cgcatgctga
    26701 tcgtcgatca gccctcggtg cggcgcagct ttcctgcgcg tctgccacct tcggtcaaca
    26761 cgcttgcgga aaactaccgg atccgcaagg gcgaaatggt acgtatcgct ggacttgaaa
    26821 gtcagcagat catcctcgaa ccgaaggaca acctgcgtta cgggcatcag ttctgggcgg
    26881 acgtgaattc cggcctgttg ctgcgggccc gcttgatcga cgaaaagggt gacagcatcg
    26941 agcagtttgc cttttccgaa gtgcagatcg gggtgcccct cgaccgagac aaggtgcggg
    27001 cccacatcgt ccgtgacccg gactggaagg tcgtcaatgc gcgcggaacg gaagtgcgca
    27061 tggaggatgc aggatgggtc ttccgcaacg cgctcccggg atatcgtcag gtcgtgtccg
    27121 tcttgcgcca gatccggcag gaccgaccgg aggcgtatca cgtggtgttt tcggatggcc
    27181 tgtcggccat atcggtcttt atcgagcctc atgcaggcaa gagttcggtg attcagagtg
    27241 gtatgagtgc caccgggtcg ttcaatatcc tgcggcggag tgcgggtgac tatctgatca
    27301 cggtgctggg cgaagtgccg gctgcggcgc tggtgcgtct ggcggatggc atcgaattac
    27361 ggaagaaatg atgcgggttc ccggagttgt gcagaaggtt gaacaaggct tcgcgtgggt
    27421 tgaggtgacg cctgcagggg gctgtggacg ctgtcacgaa ccgggtggct gcggaggggt
    27481 gagcatcaca cgggcgctgg aaacgtcccg caagacctat cggctgccca atgtaccggg
    27541 atgtgtgcca ggcgagcggg tcggcgtggt gatcgaggac ggagtggccc tgcgggcggc
    27601 gttgctgacc tacggtctgc cggtgctggg cgtcatcgcc ggcgcggcgc tcgggaccgt
    27661 gcttgcgacg ccggagcggg gcgacctggg ggcggtggtg ggcgctgtcc tggggctggc
    27721 gggtggcttt gttgccggtc gtgggcgctg gggcgagcgg acctcgcccg tgcgcctggt
    27781 gcgggatgcg gacggtacgg ggtgcggcgc atgatgcagc gcaccctggg gcgattcatc
    27841 tgggtgatgg ttctgctggg ggccttgatg ctggtgcgtc cggcgtgggc acgtgatctg
    27901 ccggacgtgg ccgagcttgc cgaacgtcag ggcgcagcgg tggtgaacat cagcaccacc
    27961 caggcaggtc gttccggtgt ccgcggagcc catccgaatc tggacgaaga cgatcccatg
    28021 ttcgacttct tccgtcgttt cgtgccgcga ggcccgggtg gcgtaccgcg gatggacccg
    28081 gacgaccgtt cgctggggtc cggattcatc gtgtcgtccg atgggtacgt gctcaccaac
    28141 gcccacgtgg tggacagcgc cgacgagatc gtcgtcaagc tcaccgacaa gcgcgaattc
    28201 cgcgcccgtc tgatcggcgc cgacgcacgc tccgacattg ccctgctcaa gatcgaggcg
    28261 tccggcctgc cgcgggtggc gttcggcgat ccagccaggc tgcgcgtcgg tgactgggtg
    28321 gtggcgatcg gtgcgccctt cgggttcgag aattcggtga cggcggggat cgtcagcgcc
    28381 aaggggcgcg ccctgccgca ggaaaatttc gttcccttca tccagacgga tgtggccatc
    28441 aatccgggca attccggtgg gccgctgttc aacatgaagg gtgaagtggt cgggatcaat
    28501 tcccagatct acagccgtac gggtgggttc atgggtttgt ccttcgccat tcccatcgac
    28561 gtggccatgg atgtgcaggc acaactgcgt cagaacggtc gcgtgcagcg cggacgtatc
    28621 ggcgtggtga tccaggaggt ttcgcgggat ctgggcgaga gcttcaatct cgcccgtccg
    28681 gccggagccc tggtgagttc ggttgagaag ggcagtcctg ccgacaaggc gggcttcgag
    28741 cagggcgata tcgtgctgcg attcgacggt cggtctgtgg ggctttcgag cgagctgccg
    28801 cgcctggtgg gcggcacccg tccgggcagc agggtggtgg cccaggtgtg gcggcgtggg
    28861 gtacagaagg atctgacggt gacggtcgcc gagttgcccg acgaaaagcc gcaaaaggtc
    28921 attgccaagg tcatgcggcc cgaagcccca cccaacaaac tcggcatcct ggtggtggac
    28981 ccgaatgcgg aacagcgccg caagatcggt gccggcgtgg tcgtggatgc cctgcgcagc
    29041 ccttcggcgc gggcgtccga gatccaggcc ggcgatgcga ttctcgcgct ggtgagtcgc
    29101 ggcacgcggg tggatatccg ttcagcggac cagttcaacc gggtcgtggc agccttcgag
    29161 ggcgggcagg cggtgtcggc cctggtccag cgaggcgacg tgcagacctt tgtacccctg
    29221 cgcacccttg agcgctgacc gccatctcac cgtgttgagt cgggcgtggt gccacctgtg
    29281 ccacgacctg ctcgacgcgc tggcgccgct gcaggtcgaa ctgggcttct cggtcgaggt
    29341 cattgacgta gacagcgatc cggaactcga ggagcgctgg aacgaagcgg tgccggtcgt
    29401 gttggcgggg gagggggagc tgtgtcgtca cttcctggac gcggcggcga tccgtgctca
    29461 tttccggcgg atcggttaga atccaagccc ttggaaggcc aacacatttc cctcgggtgc
    29521 ccgtcggcac cctttttatt gctccatgca acacattcgc aatttctcca tcatcgccca
    29581 catcgaccac ggcaaatcca cgctggcgga tcgcatcatc cagcgctgcg gcggcctgtc
    29641 cgaccgggaa atggaagccc aggtgctcga ttccatggat atcgagcggg agcggggcat
    29701 caccatcaag gcccagacgg cggcgctgag ctatcgcgcg cgggacgggc aggtctataa
    29761 cctcaacctc atcgacacgc cgggacacgt ggacttctcc tacgaagtct cccgctccct
    29821 ggcggcctgc gaaggggcgt tgctggtggt ggacgcgtcc cagggcgtcg aggcccagac
    29881 ggtggccaac tgctatacgg ccctcgacca gggcgtcgag gtggtgccgg tcctcaacaa
    29941 gatggacctg ccccaggcca acccggacgg cgcgaagcag gagatcgagg acgtcatcgg
    30001 catcgacgcc accgatgcga ttccgtgctc cgccaagacc ggcctgggtg tcgaggacat
    30061 cctcgaagcc atcatcgcgc gggtgccgcc gcccaagggc gatccggaag cgccgctcaa
    30121 ggcgctgatc atcgactcct ggttcgacaa ctacgtgggc gtggtgatgt tggtccgcgt
    30181 cgtggaaggg ggcctgcgtc ccaaggacaa gatccagttc atggccaccg gcgtgcagca
    30241 gctgtgcgag caggtgggcg tgttcacacc caagagcgtg gcacgggaaa ccctgtctgc
    30301 cggtgaggtg ggattcgtga tcgccggcat caaggaactc aaggtcgcca aggtgggcga
    30361 caccatcacc ctggccaaca agccggcggc agagcccctg gcgggtttca aggagatcaa
    30421 gccccaggtg ttcgcgggcc tgtatccggt ggaatccaac gaatacgacc agttgcggga
    30481 atcccttgaa aagctgcgcc tcaacgacgc gtcgctgcag ttcgagccgg aagtctccca
    30541 ggcgctgggc ttcggtttcc gctgcggctt tctcggcctg ctccacatgg agatcgtgca
    30601 ggaacgcctg gagcgggagt tcgacatgga tctcatcacc acggcgccca ccgtggtcta
    30661 tgaggtcgtg ctcaacgacg gtagcgtgga gcaggtctcc aacccgtcgc gcctgccgga
    30721 agcctccaag atggcggaga tccgcgagcc catcatcacc tccaccatct tcctgcccca
    30781 ggactacgtg gggccggtga tcaccctgtg taaccagaag cgggggatgc aggtagacat
    30841 gcgttatcac ggccgccagg tgcagttgat ctacgacatg cccatgaatg aagtggtgat
    30901 ggatttcttc gacaagctca agtccgtgtc gcggggatac gcgtcgctgg actatgagtt
    30961 caaggaatac cgcgcggcag atctggtgaa gctggacgtg ctggtgtcga gcgagaaggt
    31021 ggatgccctg tcggtcatcg tccaccgcag cacctcccag taccgcggcc gcgagctggt
    31081 ggccaagctg cgcgaactga tcccgcgcca gatgttcgat gtggcggtgc aggcagccat
    31141 cggctctcac atcatcgccc gcgagactat caaggccctg cgcaagaacg tgctggccaa
    31201 gtgctatggc ggcgacatca cccgcaagaa gaagctcctc gagaagcaga aggagggcaa
    31261 gaagcggatg aagcaggtgg gcaatgtgga gattccccag gaagccttcc tggcggtgct
    31321 ccgggtggac gaaggcaaat gagtccttcg cggacacctg tctgcttagg acgtgaaggg
    31381 actacgacgt gaattttgct ttgatcctgt ttgtcctgtt gctgatcagc ggggcgctgt
    31441 gggcactcga ccatttcgtc gcgcgcaagc agcgtggcgc cggtgccccg ccgccctggt
    31501 gggtggaata cggtgccagc ttctttccgg tgatcctggt ggtgttctgc ctgcgctcct
    31561 tcgtcgtgga gccgttcaag attccgtccg gttccatgct tccgacgctg ctggtgggcg
    31621 acttcatcct cgtcaacaag tggacctacg gcatccgcct gccggtcatc aacaagaaga
    31681 tcgtcgacat caatcagccc cggcggggcg acgtgatggt gttccgcttc ccggccgatc
    31741 catccctcga ctacatcaag cgcgtggtcg gtctgccggg tgacaaggtg gaatacctgg
    31801 acaagaagct tgtcatcaat ggcgttgaaa tgccccagag gccggacggg gatttcctgc
    31861 atcccgatgc cctggaccct tcccgccaag gcgatggaaa gggcgcggac cggcagacac
    31921 ggatgttctc gccccggttc atcgagaagc tcggcgacgt ggagcacagc atcctcattg
    31981 aaccggacgt accttcattc gtctcgcagg ttgtccagtt cccgcagagg gaaaactgca
    32041 cctatactag ccgcggcgtg agttgcaccg tgccggaggg gcattacttc atgatggggg
    32101 acaaccggga cggcagcttc gacagtcgcg ggtggggctt tgtgccggac gccaatatcg
    32161 tcggcaaggc cttcttcatc tggttcaact tcaacgatct gaaacgcatc ggcagtttcc
    32221 actgatcatg acgcgaaaaa accagtcggg catgagcatc atcggcatgg cgctgagcgc
    32281 cgtgctgatg ggcgccgcgc tgctcgtatt cctcaaggtc gtcccggcgg tcagcgaata
    32341 catgggcgtg cggcgggcga tttccgccgt ggcctccggt gcggatccaa ccaccgccac
    32401 ggtgtcccag ttgcgcgatg cgttcaccaa acgggcgcgc atcgacgaca tcaccagcat
    32461 cggtgcgagc gaactggata tcaccaaaga ggatggcaag atcgtcatca ccgcgtccta
    32521 ttcccgcaag attccgctgg tgtccaacgt cagcctgctg atcgacttct acgcgacgtc
    32581 ggcaccgacg ggtcgttgag cgtcccgatg aagctggatg cgttgcaggt ggccctcggc
    32641 catcgcttca accggccgga gttgctggtc caggcgctga cccatcgcag cttcggctcg
    32701 ccccataacg agcgcctcga attcctgggt gacagcgtcc tcaactgtgt taccgcgatt
    32761 gccctgtacg accgctttgc agcgttgagg gaaggggatc tgtcccgact gcgtgccaat
    32821 ctggtccggc aggaagccct cttccgggtc gccgagaccc ttcggctcgg tgagtgcctc
    32881 aagctgggcg aaggtgaatt gaaaagtggc ggtcatcgtc gcccttccat cctggccgac
    32941 gccctcgaag ctgttttcgc ggccgtcttt ctggatgcag gcttcgagac ggccaaggcg
    33001 gtgatcgacc ggctctatgc ggatgccctg gtggcgatcg acccggcgag cgccctcaag
    33061 gaccccaaga ccgcgctgca ggaatggctc cagggccgcc gcatgccgct gccccggtat
    33121 tccctggcgg atacccgcgg tgaagcccat cgccaggagt tcgaggtgga gtgcgagatc
    33181 gccggcctcg gcctgaagac ccgcggcgtg ggcgtcagcc gccgtgccgc cgagcagcaa
    33241 tccgcccagc gggcccttga gcgcctgccc aagtcatgac cgaacacacc gtttccgctg
    33301 acaccccgtt ccgcaccggg tttgtggcca tcgtcggccg ccccaacgtg ggcaagtcca
    33361 cgctgctcaa ccggctcatc gggcagaaga tcagcatcgt ctcgcgcaag gcccagacca
    33421 cccgccaccg ggtgaccggg gtcctcaccg aaggcggcgc ccagttcatc tttgtcgata
    33481 cgccgggctt ccagacgcgc cacaagaatg ccctcaaccg ggccatgaac cgcaccgtca
    33541 cccaggtgct ggcggacgtg gacctggtgc tcttcgttat cgaatccggc cgcttcggcc
    33601 ccgaggacga gcaggtggtc aagctgctgc ccgaaggtgc gcgggtgatc ctggtcatca
    33661 acaaggtgga catgctcgcc gacaaggccc gcctgctgcc cttcatggag cgcatgagcg
    33721 gcgtctatcc ctttaccgag atcgtgccgg tgagcgccga aaagggtcag aacacggccc
    33781 agctcctgaa ggccgccatg ccttacctgc cggaaggcga gcccatcttc ggtgaggacg
    33841 acatcaccga tcgcagtgag cgcttccttg cggccgaatt cctgcgggag aagctgttcc
    33901 gtcagctcgg cgaggaactg ccctacggca tgaccgtcga gatcgagaag ttcgaggccg
    33961 agcccggcct gcgccgcatc cacgcggcca tcatcgtgga caaggccccc cacaaggcga
    34021 tggtgatcgg ccgcggcggc gagcgtctca aacgcatcgc cagcgaggcc cgcgtcgata
    34081 tggaacagct cttcgactcc cgcgtcttcc tggaggtgtg ggtcaaggtc aagggcggct
    34141 gggccgacga cgagcgggcg ctgaagagcc tcggctacga ataaaccggc cgccggtctt
    34201 ccatcgggca ccctgccatg gcggcgaaac agcgtgtcga tcagcagccc ggcttcgtgc
    34261 tgcacagcta cccgtaccgg gagaccagcc tcatcgtcga ggtcttcagc cgcgaccacg
    34321 gccgcgtcgg cctggtcgcc aagggcgccc ggcggccgtc gtcggccctg cgcggcaccc
    34381 tgatggcctt tcagcccctg ctcatcgact ggagcggcgg cggcgaggtg aagaccctga
    34441 ttcgcgccga atggcagggc gggcagccgc tgctgggcgg gcaggccctg ctcgtcgcgt
    34501 attacgccaa cgaactgctg atgcgcctgc tgccgcggga agatccccat cccgaactcc
    34561 atcacgccta cggtgaggcc ctgcgcacgt tggccggtgg tgaagcgcag gaggtcgtgt
    34621 tgcgccgctt cgagctggcc ctcttgcagg agctgggcta cggcctgccg ctcgaagagg
    34681 acgtggacgg gcagcccgtc cggcccgacc ggttctacgc ctatataatc gagcgcggcc
    34741 cggtgccggt cgacgatgcc ggcatcgagg aggccgccct ggtcagcggc cgcaccttgt
    34801 tgcacatggc ccgcggcgac ttttccgatc cggacatcct cgccggcagc aaggccctga
    34861 tgcgtcgcct catccagcat tacctcggcg ggcaggtgct ccagtcccgc cgcgtcttca
    34921 ctgaactcat cgcctttccc ggagaaccgt cttgatcgaa ctcggtgtca acatcgacca
    34981 cgtcgccacc ctgcgccagg cgcgtcgcac ctgggagccg gatcccgtgt gggccgccgt
    35041 cgaggcccac ctgggcggcg ccgatggcat caccgtgcac ctgcgggaag accgtcgcca
    35101 catccaggat gcggacgtgc gcaagctgcg cgagctcacc cagatcaagc tcaacctgga
    35161 aatggccgcc accgacgaga tggtcggcat cgcctgcgcc ctcaagcccg agctggccat
    35221 gttcgtgccg gaaggccgcc acgaggtgac caccgaaggg ggcctggaca tcgtttccca
    35281 ggaggcccgc ctgaaggacg ccgtcgcccg cctcgccgac cagggcatca tcaccagcgt
    35341 gttcatcgac gcggaagtcg cccaggtgga agccgccgcg cgcatcggcg ccagggtctg
    35401 cgagatccac accggcccct acgcccacgt cttccacgac gccggccggg acgcggagag
    35461 ccctgcggtg gtggccgaga tcgacaagat cgcccgcgcc ggcgaggcca tccgcgccct
    35521 gggtctgcgc ttcaacgccg gtcacgccct caactactac aacgtccagc ccatcgcccg
    35581 cctgcccggc gtacgcgagc tgcacatcgg ccattccatc gtcagccgtt cggtcttcac
    35641 cggcctgcgc gaggcggtgc gggagatgaa gcgcctgatg cgcgaagcgg cggagcgggg
    35701 tgcccggtga tccacgggat cggcaccgac atcgtccgcg tcgcccgcct ggacgaggcg
    35761 ctggcccgtc acggcgcgcg ctttgccgag cgcatcctgg ccgagcctga gcgcgtcgcc
    35821 tggcaggccg cccgacagcc ggcgcgccac ctcgccaaac gcttcgccgc caaggaagcc
    35881 ttcggcaagg cgctgggcac cggcgtcgcc gcgccagcca ccctgcacgc cgtgcgcgtg
    35941 ggtcacgatc ccctcggcaa accggtcttc gaatacgccc cggccctcgc cgcttatctt
    36001 gcggaacggg gcctcgtcgc ccacctgagt ctttcggatg aagccgacta cgtgctcgcc
    36061 ttcgccgtca tcgaacagcc atgaagcttt cccatcgccc gcccggcccg gtcatgctcg
    36121 acgtggccgg cctcgaactg gccgccgaag agcgggacat cctgcagcat cccctcgtcg
    36181 gcggcgtgat cctgtttgcc cgaaattacg cggactcggc ccagctgcgc gccctcaccg
    36241 cggcgatccg cgccctgcgg ccggggctgg tgatctccgt tgaccacgag ggcggccggg
    36301 tacagcgctt ccgcgacggt ttcacgcgcc tgccggccat gcgccgattc ggcgccctct
    36361 gggatgccga tccggaacac gcgcgcggcg ccgcccgcca ggccggtctg gtgctggcgg
    36421 ccgagttgcg cgcccatgac gtggacctga gctacgcgcc cgtgctggac ctggattacg
    36481 gcgtgagcgg cgtcatcggc gaccgggcct tccaccgcga tccccaggtg gcggccgaac
    36541 tggcccgggc gctggctcag ggacttgcgg cggccggcct gaaagcggtc ggcaagcatt
    36601 tccccggtca cggcgccgtc gaggcggatt cccacgtggc gatcccggtg gaccaccgca
    36661 gttttgacgc ggtgtgggcc gaggacatcg tgcccttccg cgtgctcgcc cgtgaactgg
    36721 gcggtgtgat gccggcccac gtcatcttcg aaggcatcga tccgcgtccg gccggtttct
    36781 cgcccttctg gctgcaggat gtgctgcgcg gccggcttgg cttcacgggc gtgatcttca
    36841 gcgacgatct gaccatggaa ggggcgagcg tggctggcga catcgtggcc cgtgccgcag
    36901 ccgcccagcg cgccggttgc gacatggtgc tggtgtgcaa ccgcccggat ctggcggtgc
    36961 agctcctcga cgcctggcag ccgcagccga ccgccgacag cctcgcccgt atcgccgcgt
    37021 tgcgtccggc gccggggggt gtgccgggcg atccgctcac gctggcccgg tgggcacctt
    37081 actgcgaggc gcgggacgct gtgctcgccc tggcctgatc cccgcgtggc ccatccgtga
    37141 acttcgacgc caagtccttc ctgcgcaccc tcaccgagga gcctggcgtc tatcgcatga
    37201 tcggcgtcga cgagagcgtg ctctacgtgg gcaaggccaa gaacctcaag cgccgcgtcg
    37261 cgtcctactt ccagcgcacc cagctgagcc cgcgcatcgc cctcatggtg gcccaggtgg
    37321 cgcgggtgga tgtgacgccg gtgcgctccg aggccgaggc cctgatcctc gagaacaacc
    37381 tcatcaagag cctcgggccc aagtacaaca tcctcttccg ggacgacaag tcctatccct
    37441 atatcgccct gtctggcggc gagtatcccc agatctacta ccatcggggc ggcttccaga
    37501 agggcgtgcg ctacttcggg cccttcccca actcctgggc ggtgcgcgag agcctgcacc
    37561 tgatgcagaa gatcttccgc ctgcgcacct gcgaggacaa caccttcagc aaccgctccc
    37621 ggccctgcct gctttaccag atccagcgct gcaccgcgcc ctgcgtgggc aagatcgacg
    37681 cggcggccta tgcggcggac gtgcagttgg cgagcctgtt cctcaacggc cgccacggcg
    37741 acgtgatcga cgacctgtcc cggcgcatgg aggccgcctc ggagcgcctc gccttcgaac
    37801 aggcaatggt ctatcgcgac cagatccgca acctgcaggc ggtgctccac aagcagttcg
    37861 tggaaagcag caaggacgag gacgtggacg tgctcgccgc ggtggagcag ggcggtctgg
    37921 tgtgcgtcaa tctggccatg gtccgtggcg gccggcatct gggtgatcgg cctctgttcc
    37981 cggccaatgc ccagggctgc gccccgctgg atgcgctggc ggccttcgtc gagcagcatt
    38041 atctggagca tcccttgccc ggcaagatcc tcgtcaatgg cgacgtcgcc acactccgcg
    38101 aggcgctgga ggcggtggtc gagggcaagg cgcccgttgc cgcgccgcgc ttcgagaacg
    38161 agcgggcgtg gatggggatg gccgagaaca acgccggcct cgccctgctg gcgcgccaac
    38221 aggtgtccgc ccgcggcgaa cagcgtctcg acgccctgcg tgacgccctg gctctgggcg
    38281 atccaccgaa gcgcatcgag tgcttcgaca tttcccatac ccttggcgaa gcgacggttg
    38341 cgtcctgcgt ggtgtgggac ggcgccggca tgaagaagtc ggaataccgc cgctacaaca
    38401 tcgccggcat caccccgggc gatgactatg cagcgatgcg ccaggccctc acgcggcgct
    38461 acgagaaggt ggcggcgggc gagggtgcgc gaccggatct gatcctcatc gacggcggca
    38521 aggggcaact gggcgtcgcc cacgaagtgc tcaccgaact gggcctcact gaaatcggcc
    38581 tcgtcggcgt ggccaaaggc gagagccgca agccgggcct ggaggagctg gtcttcccgg
    38641 acggccgtgc gccggtgcat ctgccgcccg acgcgccggc cctgcatctg atccaggaag
    38701 tccgcgacga ggcgcaccgc tttgccgtcg ccgggcatcg cgcgcggcgg gcgaagaccc
    38761 gcaacacgtc ccggctggag gacatcgccg gggtcgggcc gacgcgccgg cgcaagctgc
    38821 tggcggcctt tggcggtatc gacggcgtgc gccatgccac gattgaagat ctgtgccgcg
    38881 tggacggcat caaccggcag ctggcggaac agatatacaa tgcgctgcga tagccttccg
    38941 acgccgacat gccgctgaac atccccaatt ccctgacctg ggcgcgcatc gccctgatcc
    39001 cgttcttcgt cggcatctac tatcttcctg cggacgtgat cggtccggac gacaagaacc
    39061 tggtcgccac cctgaccttc gttgcggcgg cggtcaccga ctggttcgac ggctatctgg
    39121 cgcgcagcct ggggcagacc tccgcctttg gtgccttcct cgatccggtg gccgacaagc
    39181 tgatggtggc ggcagcgctg atcgtgctgg tgcacctggg acgcgcggat tcactcatcg
    39241 cgctgatcat catcgggcgg gaaatcacca tctcggccct gcgggagtgg atggccaagg
    39301 ttggcgcctc ggccagcgtg gcggtggcct ttgtcggcaa gctcaagacc gctgcgcaaa
    39361 tgtcggccat tcctctgctg ctctacgatg accagctcgg gggcttcgac acccgcccct
    39421 ttggcacttt gctcatctat gtggcggcgg tgctcaccct gtggtccatg gggtattaca
    39481 tgcgctgtgc gtggccggag ctgatgaagc gcagcggctg acaccggggc gtcacatgtt
    39541 tgacagcccg attttttgaa ctataatggc ggtctgtcga gcggcagtag ctcagttggt
    39601 agagcgcaac cttgccaagg ttgaggtcga gagttcgaga ctcttctgcc gctccaaaag
    39661 atttccgggg aagccacgct tccccgtttg attctcggcg cgatagcaaa gcggttatgc
    39721 accggattgc aaatccgtgt aggtcggttc gactccggct cgcgcctcca gggtagatca
    39781 aggcggcagt agctcagttg gtagagcgca accttgccaa ggttgaggtc gagagttcga
    39841 gactcttctg ccgctccagt caaaagggga aagtgcgaaa gcactttccc cttgttcgtt
    39901 gactggtctg tttgacgtga caaaggcgct tgaaatcatt gtgccgcgcc ttggtgccca
    39961 tgggacttcg ccgcgcgatt cctgcgtgcg atggccgtcc tgcgcaagtg cctccattct
    40021 gacgtgtccg gcttccgaag cccccgaagg gcgttggggg gcagtcggcc catgccgggt
    40081 gccgggcctg tcggcgattt ttacagtagt tggcctgggg atgggcgggc ggattcacat
    40141 ttttatggtt ttcatgggct tagacagtat tagtgaattt ggcacaaggc ttgctcagtg
    40201 gaaggcactg ttcccttggt actgaatcat gaagaaagtc ctgctgttga ttgccgcctg
    40261 tgtcttctcc ctgttgagcg tgacggcacg ggccgatgcc atctttgcgc cggatgcgtc
    40321 cggaatcacc ggctccaccg tgagcctcaa cgtgagcctg ctgagcggcg acatcagctt
    40381 cggtggcgac ttcaccattg gctacgatac ggccaaactg accttgctgg acgtgctcgc
    40441 cggggatccg atgttctcgc cgctgcgcag cttcacgccc ggttcggaga gcgtttccct
    40501 ggtgaactcc tccgggagca gctacggttc cggccccttg ttctcggtgc tgttccgcat
    40561 tcttgccacc tttcccgact cgacacccgt ggatatttcc ggcgtgctgt atgacgccgc
    40621 tggcggcacg aacgcgctga ccacgatttc cccgctggtg accgtggatc aacggaccgg
    40681 aaccctgcct gagccaggcg ttttcggcct tgccggcgcc gcgctgctgg ccatggccgt
    40741 ccgccgcatg cgccggggat gagtgggccg gcgtgatcat gcgtgtgcga gtgatcgaac
    40801 attgaattga gatcgggggg cttcatgccc ctcgattttt ttgtgaaaag ggatttcgtg
    40861 ggatgcaatc atcgatcgca tccatgaagt ccggatactg atcaatacgg accgtccggc
    40921 atggctcggc ggttgcatag ggtgcatcat catggatacc aaggttcgtt cggaaaacac
    40981 cggcgcttcc tctgccaaag gttcgacgcg gcgtgagcgc gggcttgcat ggttgggaca
    41041 gttcctcgcg cccgtgctga ggcgccaggc gagccgggac gaaccggcac cccgatccca
    41101 tcgtttggcg ttcgagcccc tggagccgcg ggttctgctg gacggcacac ccagcgtgcc
    41161 gatcacccgt atcgacggtt ccatcgatgt ggccggcgaa accgaccgct acggattcac
    41221 cctcagcaac gatgcgcgga tcgtcttcga ttccctgacc aactccaaca gctttacctg
    41281 gagcctgtcg ggtccccgtg gcgatgtggt cagcagtcgc gcgttcgcgc agtccgacgc
    41341 ggataacgcg cagcccgtgc tgcaactcgc tgcgggtgac tacacccttt cagttgacgg
    41401 ggtcatggac acgaccggga actatgcgtt caggttgatc gacctgaatg cggcgcgcgc
    41461 catcgacgct gaagtgcccg tcaccgccac gctggagacg ggctaccaga cggatgcctt
    41521 tgcgtttgat gccgcggcgg gggaccgatt cgtcttcgac aacacgggcg ataccatcaa
    41581 cgccacctgg cggctgatcg atccttttgg cgcgcagatc ggcagcaacc atggtttcgg
    41641 cgatttcgat tccggaagcc tggcggcgac cgggcgttat acgctgctgg tggaaggtcg
    41701 catcggtgcg gcggccgacg caacgcccta cgcgttccgc gtgctgaagg ccggggagaa
    41761 ggtcagtacg ctcacgcccg gtgacgtgac gcgagggacg ctggcgtctg cggcccagag
    41821 tgcccgttac gatttcacgc tcggcgagac gggccagtat ttcttcgaca gcctgaccgc
    41881 ggattcgcgc ctgacgtgga gcctgcgcgg tcccctcggg cagacggtca gccctcgcag
    41941 cttctattat tccgacgcgt attacagcac tggcgcgctg gcgctggggc ccggcgacta
    42001 cagcctgtat gtctctgcga gcagtgattt cagcggcgat tacgccttcc ggctggccaa
    42061 tctcacgcag gccacgccag tcaatttcga tgtggacacg gcggcgaccc tggatccggc
    42121 taacggcagc ctgatgtaca gcttcagcgg cacggccggt gagcggattg ccctgcagca
    42181 gaccggaagc gggccctccc agacggtgtt ctggcggatc ctgggggcgg acggccagca
    42241 gattgccggt gtgaactatt tcggcggttt catggagctg cccaagcttg cggacagcgg
    42301 cacctacacc ctgatcttcg aaggggatgt gacggcatcc gggacgaccg atttttcgtt
    42361 ccgcatgatt cagtgcccgg atgtgaccga agccattgca ttgggagacg tgatcgatcg
    42421 cagcatcgac acccccggac aggtggatca cttcacgttc agcctggcca gcgcgacgcg
    42481 cgccgtgttc gattccctca gcaatgacta caacctctcg tgggggctct ataccgcgga
    42541 tggcagactc gtggcgcagc ggggttttgc ctattccgac ggctggtcga gcggcgacgg
    42601 cctcctcgat ctggcggcgg gcgactatca gctggtggtg gatccttacg cggacttcac
    42661 cggaccgttc agcttccgcc tgctcgacac gtctgccggc agcgccatca gtctgggaga
    42721 taccgtcacc gtcgacgtcg gccacagcgg acgtgacacg accgtctaca acttcgacgg
    42781 acagtctggc gaccaggtcg agttcaattt cacgtatctg tcgggcgagg gcggcgctgt
    42841 ctacagcctg ttcgatccct atggccgcag ggtgctcggt gcaacggatg gatctccgca
    42901 gcaatcccgg ctggaactga ccggccgtta caccctgctt gtcgaaggga atgtattcag
    42961 caacaacgac agcagttatg agctgttcct caactacctg tcgaacgacg ggggcaatac
    43021 gctcccggtc ggcaccgcca tcaatgtggg tgacgaggtc accggcgccg tggacgacct
    43081 cgcaaccccg gtcgtctata acttcaccct gaattccgaa accatcctgc tgttcgacgc
    43141 gctgggcgag gatgaatacg ccctcaactg gtccttgacc gggccgcggg gcgaagaggt
    43201 cagccaccgg gcgttctaca ccagtgacgg gggctactac tattacggcc tgccgccggg
    43261 ccaactcatc gccggggatt atgccctgac gctgtatggc cccgccaatc aggcctttga
    43321 tttccggttg gtggatgcgc gttcaaccgc ggctccgctt gcgttcggaa ccgagacgag
    43381 cggcacgctg cagccgggca atgccgtgca ggtctattcc atcgacgcca cggcgggcga
    43441 cgtggccaat ctcgtcgtca atagcagctg gaacggggga ctcttcgtct acgatccgga
    43501 cggcaatctc gtcgttggtc cgacatatgc gggttcgcag tcggggattt cattctcccg
    43561 gaccgggacc tatctgctgg tgctcagcgg accccgctgg cagacctggg atacctatta
    43621 cgatttcagg ctgaacctgc aatcgtcggt gatcaatgac atccagctgg gcgatggggt
    43681 gaccgggacg gccgacagct ctggcagtca ccagcgatac cgtttcaccc tgaccgagcc
    43741 gcaacgggtg ctgttcgaca gccgcaccgg caatagcggg ctctactgga acatgagcgg
    43801 accgcaaggc gtcgagcgtt ccgataccaa tttctattac gccgacggat acctttacgg
    43861 caattcgccg gtgctgaccc tcaaccccgg tacccacttc atcgacgtct ggtccacgtc
    43921 gggcgcgtcg gctgatttcg cattcagcgt cttgcccgtg tccgcagcaa agccgattga
    43981 tgtcaatcag atggtctccg gtacgcttga tccggccaat ggcatcgctg cgtacagctt
    44041 tgacggccag gccggcgatc agctcaacct gtctgccctg tcgagctccc agccctatta
    44101 caacatggcc tggcgcctca tcggtccctc ggggcagcag gtggcgtcct actatttcta
    44161 taacttcacg ccgtcagcac ttccggaatc cggcaaatac ctgttgctgg tggaaggcaa
    44221 caacagcctc tccggtcagc tcaatttcag cttccaggtc aatcgacccg tggtggtgcc
    44281 gcccgtgggc atcacgctcg ataccacgat caatggcaat cacggcacca cagcccagaa
    44341 cgaccgttac agcttccatc tcgaccagtc gacccgcgtc tatttcgatt acctgagcgg
    44401 cagcaccaat ctcaactgga gcctcgccgg tccacgcgga acgatcatca gccagcgggg
    44461 cctgtatagc gacaccgccg actacagcgg tcctgccagc atcgatctcg ccgccggtga
    44521 ttacattgtg accatcagcg ggggcgccac cggggcctat gccttccgac tggtcaacga
    44581 agagaacaag ccggtcattg cctatggcga cgtggtgaag agccagctct cgcccggtag
    44641 cgagatggag atgttcagct tcgacgccca ggcgggcgat caggtctttg tcgataccaa
    44701 gccgggcacc tctcaatatt ccagttacat ccggctgatc gatccgttcg gccgtcaggt
    44761 tgcttcgggc tactatttct cggacatcga cacggtgaag ctcgcctata ccgggcgcta
    44821 ttccgtgatt gtcgagggct tgtactacta cagcggcaac gccaccgatt acgccttctc
    44881 ggtggagaag gtcagttcca cgtcccaggc catcgacgta aatggcacga ctggcctcga
    44941 tccggtctat gttgcggcgc ccgatggcga cgcgctggtg ctggggcctt acggcagcgc
    45001 acagcgtgcc aacgacccgg cgctcaatct gaccggctcc ctgacgctcg aggcgcgcgt
    45061 caaggtgaac agtttcgcca acggctggat gccgctgatc gtcaaggacg acggcgactc
    45121 caatcaccgt acctattcga tgtggatcaa cagcggcggt tacgtgctgc tcacctccag
    45181 tgaccagtat tacgatcagt ccgtcagcac caactccggc accatcaagc tgggcgaatg
    45241 ggtagacatt gcgggggtca tcgaccgtac gagcggccag ttgcgcattt acctcaatgg
    45301 ccagctggcc gtcagcgggt cgctgcgcac caacgcggct ttcagtcacg gtggcccgct
    45361 gctgatcggt gccaccaacg aaaactggaa cggctgggcc aatttcgacg gtgccatcga
    45421 taccgtgcgc atctggaacg gcgtccggac gcagacccag atcgacacgg atcgcagcac
    45481 ggcaccggca ccggacgcgg ccgggctggt ggccgactat cgattcgccg cctcgggggg
    45541 ctcgaccctc gtcgactcca gcgcacacgg tctcaatggt gcggtggtca ataccctgac
    45601 cgggatgccc ggcacgatcg tcgggcggat cgaccgggca gggggctcgg cggcctacac
    45661 cttcacgctg accggaccgc ggaccttcta tgtggatacg ctgttgccga gccgcagcga
    45721 tttccgctgg tccctgagcg gtccttccgg cttgctggtg aatcgggcgg ccttcaccga
    45781 caccgagcag atcgtgactc tcgatgccgg cgactactcg ctggtggtgg atggcgatgg
    45841 cgacgtgacc ggcggcttcg ccctgcgctt gatggatctt tcagccacca cggcgatcac
    45901 ctacgatcag gtcgtgagcg gcaccctgac gccgtcctca ggggccacgg cctacacctt
    45961 caacgccgtc gccggcgatc gggcgtattt cgatgccctc agcaactcca cgggcaacat
    46021 ccgctggcgc ctgctggatc cgtatggcaa caccgccttc agctaccgtg ggctcagtga
    46081 cgtggacacc ctgacgttgg ggttcaccgg cgcctatacg ctgctggtgg agggctacta
    46141 cggcgacatg acgacggcct cgtacagctt caacgtgcgc cgggtcgtgg acagtgtgac
    46201 gccgctggtg atcggccagt cctcgggcct cgatccgcgc tgggtcccgg gcaagtcggg
    46261 caatgcggtg cagctcgatg gctacgactg ggtcgaggcg cccgctacgg atgctctcaa
    46321 tctgacgggt tccagcacca ccgaattctg gttcaaggcc gacgcctatt ccggtacgtg
    46381 gacgcctctc gtcttcaagg gcaatggcga ctcgaaccag cgccaggtca ccgtatggct
    46441 caataacgcc ggctatctcc acctgtccac caacacgaac ggcacctggc agggtgtgtc
    46501 caccaatgtc ggcagcgtcc agctgggtca gtgggaacac gtcgcggcgg tcatcgaccg
    46561 tatcggcaac cgcctcaagg tgtacatcaa tggcacgctg gcggttgacg ggggcctcgc
    46621 gggaggatcg gccctgtcga acagcaagcc gctcatgttc ggcagcgccc tcgagagcta
    46681 cccgcgcttc accggcagcc tggacgacgt gcgcatctgg aacacggtgc gcggggccag
    46741 tgacatcgcg accgacatga gcaccgccct gtccggcaat gaagccgggc tggtggcgta
    46801 ttaccggctt gacgagagcg ccggacgcag tgtggcggac acgtccagcc agaacagtcc
    46861 ggcgaccgtg cgccacatcc tgcaaagtct cagcgggact gcggtggaag gcgcgatcac
    46921 gacgcccggc cagcaggtca ggtacacctt ctccctggcc agcgccaagc gtctggccat
    46981 ggacagcttc accaccaaca gcaacatcta ctggacgctc acgggcccgc gcggcacggt
    47041 gatttccaat cgcaccctga gctacagcga tgccgccgag atcggcggaa cgtcgccgtc
    47101 catggacctc ggcgccggcg attatgtgct caccatcgat ggctacgcag acaccacggg
    47161 cccgtttgcc ttccggctga tggacctgaa cgccggctcg aacctggcct acgacacgcc
    47221 tgtgtccggc acgctcgatc cgggcagttc caacacggtc tatcacttta ccggtacgtc
    47281 tggggatcgc ctgttcttca acgcgacgtc cctcaccaat gcagggcaga caccctacgt
    47341 ccgtctgctc gatccccgcg gtcagcaggt gtggagcaat tatttcagca acatcgacgt
    47401 cttcagtctg gcggcgaccg gtgattacac cctcgtcatc gaggggtcgg cgtatcagac
    47461 cgtccccatc ggctacggct tcaccctgta ccgggtgacc gacgacaaga cgagcctgac
    47521 cctcgacagc accgtcaacg gcacgctcgc catccctggt cagaccgatc agttctcctt
    47581 ccatctggcc caggacacgc gggtctattt cgactccctc accgccaaca gcagtcttac
    47641 ctggaccctc gcgggggcct cggggaccgt ggtaagccgc aatttctact attcggattc
    47701 cgatggcctg ggcgggacca atccgttcat gctgctgcgg gctggcgact acaccctgac
    47761 ggtggacggt tccgggaaca cgaccggggc ctacgcgttc cggctgctca cggcggcctc
    47821 cgcagaggcg atcaccgtcg gcaccgaggt caataccagc ttcgatccgt acgcctcgca
    47881 tctctattca ttcgatgccg cggcgggcga tcaggtgatg ctcgacttcc tcgaccgcac
    47941 gagcagcttc tacccgatct ggcggctctt cgatacggac ggacagcaac tcttctacaa
    48001 ctacctctac aacgacagcg gcaccctgac cctgcccaag gccgggcatt atctgattgc
    48061 catcgaaccc cgcatctatg atggcagcgg gacggcacgc ttccaggtca cgtcccaagg
    48121 ccatgtggac ctgcctgcgc cgcccgtggg tgacccgatc accctgggcg cgcaggtcaa
    48181 tggcaacctg acctccggga cggacagcaa gacctacgcc ctggatctga gcgatgccag
    48241 gcgtctgtat ttcgatgtcc tgagcaacac gccctacgtc cagtggagcc tgaccgggcc
    48301 cagcggcgcg ctggtcacca gccgctattt ctattcctcg gacggcagca acgccagctc
    48361 ctacggcaac acgcccattc tggatctggc ggccggtcac tacactctga cggttggcgg
    48421 cagcgctggc gcctttgcct tccgcctgct cgatcttgtg gcggcgaccg cggtcaccct
    48481 cgacagcagt ggcaaggcgg acgtgtccgg tcagctttcg ccgcgcagcg agacggatgc
    48541 ctacagcttc accgcgacgg cgggcgagcg ttattacttc cagcgtctgc aggggaacta
    48601 caacagcgcc ttctggcgcc tgatcggacc ctccggacag gtggtcatgg ggccgtccga
    48661 ccagtggtac gacgtggaga acgtcaccct gccggaagcc ggcacctata ccctgctcat
    48721 cgaagggccc tactactaca cggacacggc caactacagc ttccgcatct cgcgcatcac
    48781 ggacccggcc ccggtcacaa tcgatctggg tcaggtcgtc agcggcagca tcgattcggc
    48841 ggggcaggtc agtcattaca cgctggtcct cgaccaggcg cggcaggtct atttcgatac
    48901 cctcggatcg gcaaacgacg gcgcggtcgc ctggacgctg agcaattccc tgttgggcag
    48961 cgtggtgaac agccgcgcgt tcagtcagag caatgcctac gaccgctccg gcaacggcgt
    49021 gctgctcgat ctggcggcgg gcacctacga tctggtcgtt gatggcgcgg gcagcttcac
    49081 cggggcctac aagttccgcc tcctcgacac cacggcggcg gccaccacca tcaccctcga
    49141 cagcaccgtg gttgccaacg tggacccggc ggacagcacc cggttctacc gtttcgacgc
    49201 caccgccggt cagcgtgtgt tcttcgacat gctgggcagc accggttcga caccttattg
    49261 gcgtgtcatc ggcccccatg gtgaggtggt ccaggacgtg caatggggcg ggtcggaccg
    49321 ggatgtgacc accctgccgt ccagcggcag ctatctgctg atggtggagg gcgtccgcaa
    49381 tgcgacgggg acggcgtccc tgagcttcaa tctccggacg gtcaccgaca agacggacac
    49441 cctgaccctc ggcagcacgg tcaatgcgtc catcgacgcg ccgggccaga ccagccgcta
    49501 caccttcacg ctgggtgaca ggaagcgggt gttcttcgat gccctcaact acaacggcaa
    49561 tgtcacgtgg tccctgaccg gaccgcgcgg tgttgtggtc agcagtcgcg ccttcggctc
    49621 gtccgacggg acgaccaatc agccctggct ggacctgccg gccggcgatt atcaactcgt
    49681 cttcgatgcc cccggcgatt ccaccggggc cttccagttc cggctgctcg acgtggccca
    49741 ggcgacggcc atcacgccgg gcacggtggt caccggggtc ctcagcccgg tggccgagat
    49801 gtccctctat acgctggccc tgcaggccgg tgaccgcatc tacctcgaca gcctcggcgt
    49861 ggaccgcaat gcctacgatc tgtcctggcg tctgctcgat ccctacgggc gcatcgtggc
    49921 gagccccggc ggcttcgtcg acgtggatgt cacgacggcg gccttcagcg gcaattacat
    49981 gctgatcgtc gaaggtcagc ccgccgccgt cggcaccgcc aattacagct tcaacatctc
    50041 tccggcgccg ctctccaagg tcgtgcccat cgccatcggc gatcagccgg gcgcggacct
    50101 cgtcgtctcc aacctggcgg tcacgccggt gtccagcgac gtgcattcgg gcggtcaggt
    50161 cgtcctcagc tgggataccg tcaacgccgg caccctgccc accagcggca gctggaagga
    50221 ccagatcgtc gtccgcaaca gccgcggtga actgatctac acccagctcg tcgaccacga
    50281 tgaagcggtc gatggcccgc tggcgtccgg cgccaagcag agtcacagcc ttaccctgac
    50341 gttgccggac ggcaaccgcg gggcgggcac cctcagcttc cgtgtcacca cggacgtgga
    50401 gaacaccgtt gcggagctcg gctccggcgg cgctgcggaa ctcaacaacg cggccaccgt
    50461 gagcgtcgcc tcgacccttt cgccctacgc cgacctgcag gtggccaacc tgaacgtcag
    50521 cccggcggcc gggtgggcgc cggggtccca ggtcacgctc agctggcgtg acgtgaatgc
    50581 cggcgtgggt gccacctcgg gtggctggac cgacagcatc gtggtgcgta acctcagcac
    50641 gggccggacc ttctatgtgg gcagcctggc ctatgacgcc acggcaaacg gcgatctggg
    50701 cgcgggagac ggtcgcgatc ggcaattgac gctggtgtgg ccggccgacg cgcagggcat
    50761 cggccgcttt gaattcagcg tcgtggccga cgcccagggc cagatcttcg agaacaacgc
    50821 caacgattcg gcggagagca acaacacggc ggccctcacc gtcacctccg cgccggatct
    50881 cgtggtgggc aacctgcgca ccaccccgtc cagcaccgag tcgggttcgt cagtcacggt
    50941 gcaatgggac accgtcaacc agggcctcgc cggaaccctc ggcagctggg tggagcgggt
    51001 caccgtggtg aaccccgcga cgggcgagac cctcgtggat acgcgcctga cctacgatcc
    51061 gagcgtggcc aacgccggca acattgccgc cggtgcaaat cgcaaccggt cgtatacctt
    51121 cacgctgccc gaaggccagc gtgcagtggg cgcgctcaac gtcaccgtca gtctggatta
    51181 caacggccag ctggtcgaat ccgacgaaac caacaacagc gccaatgttg tcctcaacgt
    51241 ggcgaagaag aaatacgccg atctggcccc ggtcagcctg gaggcgccgc ccagcgcccg
    51301 tgggggcgac accgtgaccc tgcggtggac cgtcaccaac cagggcgatg cgcccaccgg
    51361 gacccagtgg accgaccgca tcgtgctgtc cgccgatggc atctacggca acgccgacga
    51421 cgtggtgctg gtcaccgcgc ctcactttgg cagtctcgca ccgggcgagt cctacaccca
    51481 ggagcgccag gtcgtcctgc cccagcgcgt agatggcgct tacaagctgt tcctgcgtac
    51541 ggacgtgaac gccgaggtgc tggaacccga tacccgcgcc aacaatctat tcggccccaa
    51601 ggcgatcaac atcgccgcgc cccatgccga cctggtcgtc gaggccgtct cggcgccgac
    51661 tgcggcccag agtggcgatt cggtgaaggt ctcgtggcgc gtgcgcaaca ccggtgactc
    51721 caccacggac tcaagccagt ggaaggacta cgtctacctg tccagcgaca atcagctcga
    51781 cgcgagcgac atcctgctgg cccaggtgga tcatacgggc cagttcctcg ccgttggcga
    51841 cagctacacg gcgtcggcaa acgtcttcct gacgtacggc ctgaccggca attacagctt
    51901 catcgtccgc accgatgcgg aaaaccgggt cttcgaggcc aacttcgaga ccaacaacac
    51961 cacggccgcg ctgcagacca ccaaggtcac gccggcgccg gtgcccgatc tccaggtgag
    52021 cgccatcacc ctgcctgcgc agatcgtgtc cgggcagacg gcccatgtgg catggaccgt
    52081 caacaacgga gggggtggca cggcgaaggg tccgtggacc gaccgcgttt acctgagcac
    52141 cgacgggacc ctcaacagcc ccaccttcct gggtgatgtg gcccacaccg gcaatctgga
    52201 cgccggcggc agcctgtcgt cgtccctcga tttcatcgtg ccggccctgg ctgacggcaa
    52261 ctaccgcatc gtcatcgtga ccgatgtgaa tggccagatc tacgaaggcg gcagcggaag
    52321 tgccggcgaa aacaaccagt ttgccgccac ggtgcctgtg gccctcgtgc atccggacct
    52381 ggtgccgctc cagctcgatg cgccgtccac cgccgtatcc ggcaatcgcg tgacactcac
    52441 ctacacgatc cgcaacgacg ggaccggtgc ggcgggcggc gactggatcg accggatcta
    52501 cctctcgcgc gacaccaccg ttgacgccag cgacaccctg ctggccaccc gcgccgtgtc
    52561 cggcccgctg gcggcggggc agggctatgg cggcagcgtg gacttcgatc tgcccctggg
    52621 cgcccagggc acctattacc tgctggtggc ggcggatgcc aacaaccagc tgcccgagcg
    52681 caatgccgag gggaacaacg ttgcttcgcg tcagctcgac gtgaccctgg accagtatgc
    52741 acagctcgtc accaccgacg tccaggcgcc gaccctgacc attggtgacc cggcccagat
    52801 caccgtgagt tggaccgtcg ccaaccaggg cacaggggtc ggccgcacct ccacctggac
    52861 cgacgccctc attgcgtcca ccgacagcat cgttggcaac agcgacgaca aggtagtggg
    52921 cgagttcgtc cataccggcg gcctggccgt tggcgattcc tacagccgca ccgaaacctt
    52981 cctgctgccg gcctacttcc agggccgcta ccacttcttc gtcaagaccg acgccaagaa
    53041 cgcggtcttc gagaacggca acgaagccga caacgccaag gagtcgccgc agatcttcga
    53101 cgtgatgtcg attccctacg ccgacctggt ggtaagcgcc gccagcgcgc cgaccacggc
    53161 cagttccggc cagaccatga gcgtgacgtg gacggtcgcc aacacgggca tcggccccac
    53221 ggacattccc ggctggaacg atgaggtcgt gctcacctcc gatgcggcag gcaacaacgt
    53281 ggtggccaac ctgggcagct tcacccacct gggtgtgctg gctgcgggcg acagctatac
    53341 ccgcacccag gacgtatcga ttcccaacgg cctgtcgggc agttactacg tacgcgtccg
    53401 ttccttcggg cccttcgagt tcgtctacac ccagaacaac acccgcgtcg ccgggccggt
    53461 gcagatcgcc ctgtcgccgt cgcccgacct gcaggtgacc gacatccaga cgccgtccac
    53521 cgccaacgag ggtgactaca tcgacgtcaa gtggaccgtc accaataacg gcgccgccaa
    53581 ggccgacggg tcgtggaccg atatggtcta catgcgcaag gtgggtcagc ccaacgatcc
    53641 gggcatctac ctgggcacct tcagtttcga cgctggcggg ctggatgccg gcaagtcata
    53701 tacccgtacc gagcgtttca agctgccggc acggaccgaa ggcgtgtatc aggcggtcgt
    53761 ggtcaccaat gccaacgcca acagcggcgg cgtctacgaa tacggcacgg cggccgccaa
    53821 caacaccacc gccgatgatc agatcctgca ggtggccctg ctgccgcggc cggatctcca
    53881 ggtcggtacc gtcgtggcgc cgtccagcgc gtcggcgggg gcggccgtgt ccctgcgctt
    53941 cagcatcatc aaccagggca ccgtgcccac cgccacgccc cactggaagg acaacgtcta
    54001 cctctcgctg gacaacaagc tcaccggcga cgatctgctg atcggcagct acgacaacgg
    54061 tgccgccctc aatccgggcg agcagtacgc caccgagagc ggcagcctgc agatcccgat
    54121 ccgtttccgc ggcgacgtct tcctgatcgt ccaggcggac ggcagcggct cggtggacga
    54181 ataccccaac gacgccaaca acatcaccgc ggtcccgctc tacgtcaatc cctacccgct
    54241 ggccgacctg gtcaccggtg gcgtggtggc gccggcccag gccgtggaag gggccaacat
    54301 cgaggtgcgc tacaccgtcg ccaacaaggg cagcggcacc accgcgagca attccggcgc
    54361 cgcctcgtgg accgacacca tctggctgtc ccgcgacaag acccgcccca atgcaggcgg
    54421 caacagcgca gtcatgatcg gcaccgtgca gcacaacggc gccctcaagg tgggcgagag
    54481 ctacgacgtg gtgaccaacg tcaacctgcc gtcccatctc acctccggca cctactacgt
    54541 caccgtgtgg agcgattcct acgatgtgat cctggaggac accctcgcca gcaacctcaa
    54601 cccggacgat ccccacgagc tcgacaacaa caactacaaa gcccgggcca tcgacatcat
    54661 tggcggcttc gaaccaccgc cgcccgacct gaagatcacg gccctcacgg cgtcgcccca
    54721 ggcgtccacg agcagtgatc tcacggtcaa ctggaccgtc accaatgccg gcgccggcga
    54781 ggcgtcgggc aagtggtatg acgtcttctg gctgtccgac aagccggacc tcaatgcgtc
    54841 gggtgccaag cagtggtatc tcggctcgat cctccacgat gggggcaccg cgcccggcgc
    54901 cagttattcc gcgtcggcca ccttcgcact gaacccggcc gcggcgggca agtacctcat
    54961 cgcctacacg gatttcgacc cgtatgtcgg caaaccctac gacgtcgtcg aaggcaatga
    55021 ggacaacaac acccgtgtca ccgacaccag cgtcaccgcg gtgccggccg acctgaaggt
    55081 cgtcaccatc gccgcgccgt ccgagaactt ctcgggcgag aagaccaccg tcacctggac
    55141 cgtcaagaac tacggcggcg acgtgtggtc cggcacgcgc tactggctgg acagcgtcta
    55201 cctgtcgccc gacccggtct tcctgcccga gcgggccacg gccctgggca cctttgtcca
    55261 cagcaacaag gacggtctcg cggccggcgc cagctacacc cagagcgctg acgtggtgct
    55321 gccccgtggc atcggcggca attatttcct ctacgtcatc accgacgcca acccctacga
    55381 cccgcgcttc cccaacggcg agacggatca gggcgacaac gcgtatttcc gcgacgtcta
    55441 ctaccccggc agcgcctacg aaggcgtggg cggcgagcag aacaaccgcg gtatcggcaa
    55501 catccccgtc acctaccggg aacccgacct gaaggtcact ggcctggtgg tgccgccgga
    55561 tgcccagtcc ggcaagacca tcacggtcac ttatacggtc accaacatcg gtaaccggga
    55621 cacccgcgag aacgcctggt gggaccgcct gttcctgtcc aaggacggct ccctcgacac
    55681 ctacgacctg caattgtcgg aggcgcgtca ctacggcatc ctcaagaccg gcgacagcta
    55741 caccggcacc gccaccttca agctgcccga cggcatctcc ggcaacttca gcctgctggc
    55801 cttcaccgac tccgaggcgg atgcccgcct gtcctggtgg aacagcaaca tcgtgcccga
    55861 gtaccggggt gtgggcatcg acgccaacag cgactcggtg ccggaattcc gtggcgaggg
    55921 caacaacatc accgtcgccc ccttgccggt ggtcgccgcg ccgctggccg atctgcaggt
    55981 caccaccgtg acgacgcagg cccacatcac ggtgggcaac gacttcaccg tgacctacac
    56041 cgtcgccaac ctgggcggtg ccgacacgcc gcccgagcag gccgagtggg aggactacat
    56101 ctacctgtcc cgcgatccgc tgctggacct ggacagcgat cgctacatga cgaccgtgcg
    56161 ccacgtgggt ggccttgcca agggcgccag ctacacggtc aaccagaccc tgcgcgcgcc
    56221 gctggacgtg accggcgcct ggtatgtctt cgtcgtcacc gacccggccc gcggctcggg
    56281 tcgcggcaag gtcttcgagg gcggcctgga acggaacaat gccaccccca gcacgcccgc
    56341 catcttcgag atgccgcccc cgtccgatct gcaggtggac gacatcgtct ttggtggcaa
    56401 cgggcagtcg ggcgagaaca tccacatgga gtggacggtc tccaaccaca gcaccaatcc
    56461 cacctccacc agctggaccg acgcggtcta cctgtccgac gacaacacct gggacatcgg
    56521 cgaccgcctc ctcggcaagg tcacccacac cggcgtgctg ggccaggacg gcagttacac
    56581 ggccagcctc gacgccctca ttccgcccac caagccgggc cagtaccggg tcatcgtgcg
    56641 gccggacatc tacaacgagg tctatgaggg cccgtaccgc tctgcaggtg aggccaacaa
    56701 cttcaccacc tcgcccaatg tgctcaacgt cacggtgaat gaactgcacc tgggcgtgta
    56761 tgaggacacc accctctcca ccggccagag ccgcctctac aagctcaccg tggggcaggg
    56821 ccagaccctc aaggtcagcc tcaccaccgg cgaccaggac gccgccaacg agatcttcat
    56881 ccgctacggc gacgtgccgg acggcttcca ttacgacgcc acctacgaca atccgctgca
    56941 ggccaaccag accgccgtgg tgcccttcac caagccgggg gattactacg tcctgatccg
    57001 cggccatgcg gaaacccagg cgaacacccc ggtgcgcgtg ctcgccgaag tcgtaccctt
    57061 cgccatcacc agcgtccacg tggaccaggg cggcgacagc cgctgggtga ccttcgacgt
    57121 gcgcggcgcc cagttcgccg acaacgccat cctcaaggtg gtgcggcccg acgtggccga
    57181 atacgaaccg gtgaagtggg acgtcatcga ctccacctgg atccgcgcca ccttcgactt
    57241 cacccatgcg ccgctggggc tgtatgacct ggaggtcatc aacccgagcg gcgacaaggc
    57301 catcatcgcc taccgcttcc tcatcgagcg ggccatggag accgacgtca ccatcggcct
    57361 gggcggcccg cgcgtgctgg ctgccggcga gaccggcacc tacggcgtgg ccctgcagag
    57421 cctcaccaac gtggacacgc cctacgtgcg cttcaccttc ggcgtgccgg agatgggcaa
    57481 caaccagtac gtctacaacc tgcccttcct caagtactac aacgacctgc gcggccagcc
    57541 cgacaccggc ggcgccgacg tgccgtgggc gagcctggat tccgccacca acaccaccgg
    57601 gcagatcctg tcgtcgggtt atgcctacga cgtgatcgcc ggcggctatg ccggggcgac
    57661 tttcaacgtc accacctacc cgctgctcaa ggccctgtcc accctcaact gggatgccct
    57721 gcgggtcggc ctctacgaga tgtacccgga cctggagaag atccaggccc tggcgggcgg
    57781 gccggagtcc atgcagacgg cgctgccggc ctactgggat ctgtggcagc aggccaactc
    57841 cgaagacggt ctgccggatg attgcgtgat ccccttcatc ccctaccgct tcaacatcgt
    57901 gggggccgcc acccccatga cccgcgacga gttcatcgcc gaccagacca ccgaagccct
    57961 caagctgcgc gccgccatcc tcggcgacac cgacctggac gccaaaatcg ccgccaccgg
    58021 caccgatgac accacgctcg ccgagaagcg cgccgccatc gccctgcgca acctggcctc
    58081 cgatgcggac ggctgggtga atgcctacct gggcgccctc gagcaagtgg gcatcctgcg
    58141 tccggtggac gaggcgccac cgatccgtca gaacaccaag gtgatgagcc tgatgagcac
    58201 cctggcgacc ggcatcctgc tcggccccgt gggtgaccag atccgcacca ccggcgatct
    58261 cgtcgaattt ttcgccaaga tcaagacctg gtacggcgac acgcccaaga ccctggccgc
    58321 catcgaccat tacgaacacc gggagagcga ctgcctggac ggcgagatcc ccgtgccggc
    58381 cctgcccaag tacgcggact acaacctcaa cctgtcgcac ccgacctact tcgagacctt
    58441 caacatcttc gtgccctacg tgggcttcaa caaggacggg gcgtccgctg ccaccattgc
    58501 gctgcccgac tacgcctcgg tggacgcgtc caaccagctc accgcgctgg acttctcgcg
    58561 cctctacgcc caggccatca gcgacagcca gctggcgtcc atctccggcc cgcaaggcta
    58621 cggcgtcgat cagttcctgc ccgtcggtca ggcgctgccc tataccgtca agttcgagaa
    58681 tcctgctgcg gcgtccagct ccgtcaccga gatccgtgtc gccagcgccc tcgatccgtc
    58741 cctggatccc accaccttcc gcctgggtga catcaagctc ggtgacgtga cgatccacat
    58801 cccgagcggt cgcgccctgt tccagggcga cttcgacctg cgcaattcca agggctacat
    58861 cctgcgcgtg tcggcgggca tcgacccgac caccagcacc gcctcgtggg tcttccaggc
    58921 catcgacccg gacaccggcg aggtcctgca ggacgccacc cgcggcctgc tcctgcccaa
    58981 tgcagcggac ggccgcggta ccggccagct cggctacacc gtccagccca aggccgacct
    59041 gccttccggc acgcagatct ccgcccaggc cgtggtgcgc ttcaacgcca tcgccccgca
    59101 agagacccag aagctcaccc agaccctcga cacccaggcg ccggtgacca ccctcaccgc
    59161 cacgccggtc accgccggcg gcaacgatta cgaggtgcgc tggagcagcg cggacgaaga
    59221 ccgcggctcc ggcgtcgccc acgtcaccgt gtacgtggcc gaggatggcg gcgacttcaa
    59281 gatctggctg cgccagacca ccgacacctt cggtgtctat tccggcaagg ccggccacac
    59341 ctacgaattc ctggcccttg ccacggacaa cgcgggcaac cgggagcaac ccccgggcag
    59401 cgtgcaggcg cccgatgacg gctccggcgc caacctgggc ggcctgccga gcttcggcga
    59461 gaccacccag gatctgccgc cgccgccggc gccggccacg ccgccgacca atccgctgtt
    59521 catcgaggcc cagtcgcaga cgccggccgc cacacccctg tccaagctgc ccgagttcac
    59581 cgaggtcgag cggcccttca cggcccaggc cttcgccacc gacttcgtca gcagcggcgc
    59641 cggtgtcggt gcgctggcca tgctggagct gcccgacggc tcggtgctcg ccagtggcgg
    59701 ccccaaccgg ggctggctgt accgcatccc ccacgacggc ggcaaggccc tcacgccctt
    59761 cgtcgaactg gacgacccca tctacgacct ggccctggac gacaacggcc agctgtgggc
    59821 gaccaccggc ggcaacgacc tgctcaagct ggatctggcc accggcgcgg tgctcgcgcg
    59881 ctacggcacg ggcatcaccc aggccctggc gatcaaggcg ggcacgggtg agatctacgt
    59941 ctcgtccggc gacggcatcg agatcttcga cccggtcaag gcgacgttcc gtcacttcag
    60001 cgacgtgcgc gtcgatgacc tcgccttcgc gcccgacggc accctgtggg gtaccacctg
    60061 gcccgaccgg ggcgacgtgg tcaccttcga caagcgcgga cgcgccaccg tgcaggtgcg
    60121 cctggacggc gaggtggatt ccatcgcctt cggccaggtc ggcacccgcc tggaaggcct
    60181 gctcttcatc tccgccaatt ccaccaaggg cgagcagggc gccgccctca ccatggtgga
    60241 tctggtgtcc aagcgcagcg tcgagatcgc ccggggcggt tcccgcggcg agaccgtggt
    60301 ggccaccaag gacggccgcg tgctggtggc cgagacccac cagatcgacg tgatcagccc
    60361 cattgtcgcg ccacgggtcg tcgccaccaa tccgcccgac caggccgtgg tgaccctgcc
    60421 gctgacgacc ctgacggtca ccttcgacca ggatatgtac gcgggcagcg ccacggacag
    60481 cgcctccgtg ctcgacccgg ccgtctatca ggtggtccgc cagaacggca gcctggtgga
    60541 catcgccagc gtcgcctacg atgccaccac ccgccgcgtg accctcacgg tcgccggtct
    60601 ggacccggat ctctacgatc tgcgcgtgct tggcaccgtg cgcagcagcg ccggcatggc
    60661 cctgggcggt acctacatct ccagcttcgt ggcggtgcag gacttctcct cggtggtgag
    60721 cctgcagttc gaccgggtgc gcagcgaccg cggcaccggc gtggtgtcct acgacatcac
    60781 cgtcaccaac accgccgact acgacctgct ggtgccggcc gcgctggtca tcgacccggc
    60841 ccgtttcttc cagggacggc ccatgggtgc ggtgagcccg cagggagcga acggcctgtg
    60901 gtacatcgac ctgtccggta agctgcccgg cggcgtgctc aaggccggtg catccatcgc
    60961 caccttcacg gtgggcatca ccaacagcgc cagccagcag atcagcctgg gccacggcat
    61021 ctacgccgtg ccctatccca accagaagcc ggccttcacc tcgacgccgg tgacggccgc
    61081 cacggccggt caggcctacc gctatcaggc ggtggcccag gatccggacg gccgcgtgat
    61141 cacctacctg ctggtggatg ggccggcggg cctcaccgtg gacagcacga cgggcatcct
    61201 cagctggctg cccaacgtcg ccagccccgc cacgtccacc gtcaccctgc gggcctatga
    61261 cacccgtggt ggcttcgaca cccaggtctt caacatcgag gtggcgggcg ccaaccaccc
    61321 gcccaatgtc tccgacctgc ccgccgagat cgacgggaag gaaggccaga gcctgctcgt
    61381 cggcctcgcc gccgaagatg tggacgaaga cttcctgcac tactacgccg acaacctgcc
    61441 gccgggcgcg gtcttcgatt cgcagctcaa cgccctggtg tggacgcccg acttcacgtc
    61501 cgccggcacg tacaccaacg tcaagctcat cgtcagcgac ggcaagagca cggtgacgcg
    61561 gtccttcaac atcgtcatcg ccccggacga cgcgccgccc gtcctcagcc cggtgcccga
    61621 gcgcaccctg cgcgaaggcg atccggtgcg cctgcagctg catgccacgg atgccgacag
    61681 cggcgacacc ctcagcttct acgccgacgt cctgcccgcc ggcgccttcc tggatccgaa
    61741 tacgggcgtc ttccaatgga cgccgagctt catccaggcc ggcgactaca gcctcaagtt
    61801 cggcgtcagc gacggcacca agcgcaccga gaccaccgtc aagttccacg tcctcaacgt
    61861 gaatgcggcg ccggtcttcg acgaactcgg atcgtggatc agcttcgagg gccagaccct
    61921 ggccttccgc gccttcgcct tcgacccgga caaccccggc tacgttgccc aggaccgcct
    61981 gcccagcggc accctgaccg aactggaagg ggccgcggcc acggtcagct acaccgtgtc
    62041 cggcctgccc gacggggcga gcttcgatcc ggtgacgtcc atgttcaagt gggtgccccg
    62101 ctacgatcag gcgggcagct acctggtgca cttcaccgcc accgacgacg gcaacggcac
    62161 cggcacgcct ctcacctcgg acatcgtcgt gcccatcacc atccgcaacg ccaaccgggc
    62221 gccggagatg gccgagatcc cgacccagac cgtgcaacgc gacgcagtgt tcgagctgcc
    62281 cgtgacggct accgatcccg atggcaatcc gctgacgctc actgtcaagg gcctgcccgc
    62341 cttcgccacc ttcaccgaca acggcgacgg caccggcagg ttcgtcatcg cgccccattc
    62401 cggcgaccgt ggtgactacg agatcgacct caccgccacc gacaacggcg acggacaggg
    62461 cgccctctac gcccagtccc tgacccgccg cttcatcctg acggtggtgt ccgcctccga
    62521 ggcgccggtc atcgcgccca tcggcgacaa gctggccctg gttggccaga agctcagctt
    62581 ctacatcaac gccaaggatc tggaccagga tgcgctgggc ttcaccgcag acgccgcgcc
    62641 ggccggcctg accatcacgc cgcagcccca atacggccgc gccctggtgg aatggacgcc
    62701 cgacagcacc gtgctgggcc agaccttcac ggtgcatttc aaggtcaccg acaacggcaa
    62761 cagtggtgcc acggcgccca acaccaccga gcaggtggtg cgcatcaagg tgcgtgacag
    62821 caacgccgcg ccggtcctgt cgcccatcgg caacctgagc ctcaaggaag gccagccctt
    62881 ctcgctgcag ctctccgccg tcgatccgga tggcgatccg ctcacctaca gcgtcaccaa
    62941 cctgccggcc ggcgccagct tcgacgcggt caaggggctt ttcacctgga cgcccaacat
    63001 cttcagtgcg ggcgactacc cgaacgtgcg cttcgccgtc agcgatggca tggcggagag
    63061 cgccgagacc gtcacgatct cggtgcagaa caccaaccag gcgccgatcc tggtgtccct
    63121 cttcccgcag accgggcgcg agggcgcgca ggtccagttc cagctcaccg tgggcgacgt
    63181 ggacggcgat ccggtcgtct tcaaggccct cacggcgctc cccgacggct cccgcttcga
    63241 ctcgcgcacc ggcctcttca agtggacgcc cacctacgat caggcgggtg actacagctt
    63301 caccttcgag gcccgcgatc ctgccggcgc caccagccag accaccgtca gcctggccat
    63361 tgccaacgtg aaccgggcgc cggtgctgac cgtctccaac cacgtggcgc tcctcggccg
    63421 gcccctcagc ttccagcttg gtggcagcga cgccgattcg ggcaccacgc tcaccttcag
    63481 cgccaccggg ctgcccgagg gggccaccct cgatccgacc actggcgtca tcaactggac
    63541 gccgggcgcc ggtcaggctg gggactacct ggtcatggcc tccgtgtccg acggcatgac
    63601 gagcaccgcc ctgccgctgg tgatccgcgc cgccctcacg gcgccggccc ccagcgtgat
    63661 catcgagaac acgcccagct tcccggcggt gcccggccag gacgtgctgg tccacgtcat
    63721 cgccgagagc tttggcaaca tcgccggact gcgtctgttt gccgatggtg tcgagcttcc
    63781 gctggatgaa ttcggccgcg cccacgtcca ggcgacccag gtgggcaaga tcgccctgcg
    63841 cgccgaggcc accgacgtgg atggcctggt gggcagcacc acgtccgtga tccgcgtgcg
    63901 cgatccccag gacaaatccg ccccgacgcc gtccttcgcc agccagctgg ccttcgcgcg
    63961 cctcaccgac ctcacggcca tcaccggcag caccggcgag gccaatgtgg atgcctggac
    64021 gctggagatc gcgcgctacg gcgacgagca ctacacgacc ctggccagcg gcgatggcgc
    64081 ggtgaacaac gccgccttca tgcagctcga tccggcgacc ctcgccaacg gcatgtacac
    64141 cctgcgcctg cgggtcaccg acctgggcgg gcggcgcggc gaagccagcg tcgacgtgga
    64201 agtggattcc gccaccaaga cgggccgtct gcagcgcaat gaagccgatt tcagctggag
    64261 cgtcggcggg cacaccctcg acttcctgcg cagctacgat tcgctcaaca cctctgtcgc
    64321 cggcagcttc ggcaacggct ggcggctctc cttccgggat gtggatctgc agaccgacgt
    64381 ggccctcacc gggcgcgagg atgcgggtga actgggcacc ttccaggacg gtcagcgggt
    64441 cgtcctgacg ctgccgtccg gtgagcgggc agccttcacc ttcgtgccgc agaaacacag
    64501 catccccggc cagacctggt acaccccggc cttccaggcc gaatccgcca gcggctggca
    64561 gctcgtcgtg cccgacacca agctgcagcg ggccggctcc agctactacg atctggtcag
    64621 cggcgtgggc tacaacccgg ccaattccgg cgacacggac tacatcctgg tggcgccgga
    64681 cggcacccgc tacagcatcg ccgacggccg catcgacgac gtgcgcttca ccgacggcgt
    64741 gcatgtggcc gtgagcgaca gcggtctggt ggccagtacc ggcgagacgg tgcaattcac
    64801 cctcaaccac gccggggcca tcgaacgggc cgccctgtcc gacgggcgtc agtacctcta
    64861 cgactacgac gcccagggcc ggctcgtttc agtccgcaac ctggaggcgg ccacgtcgct
    64921 gcggtatggc tacgaagcgg gcgccctcgg ccgcctgacg ctggtcaccg gcagcggggc
    64981 aggggccctg tacacctatg gtccgtccgg agtcggccag gcgcccatct ccggcgatct
    65041 ggggtcctcg gtcaattacc ttgcgactcc ggtgaatggc agcctgatcg cgggcgaggt
    65101 ggatcactac gccttcacgc tccgtccgtc ggagctggcc tccaccgcga gcggctcggt
    65161 gtatctgggc atcgtggtcg aaggccataa cggcctcgcg ccggtcctgc cggtcatgcc
    65221 cggcctcacc gccatcgcct cgtcggtggt gggcaatcgc gccttcgccc tcttcaagct
    65281 cgatgccgcc ggcctggcgc agatcgacgt ggccggcagc ggcgtgggtg actacaccct
    65341 gagcctgttc gtggcgggtg acgccaaccg ggacggcaag gtcgacgggg ccgacgccat
    65401 cgccatcgcc gccgccaagg gcgcggtgcg gggcggggcc ggctacagcg aggccctcga
    65461 tgcggacggc aacggcgtca tcgaaagcgg cgacctgcag ctcctcatgc agaacgcggg
    65521 cttcacgccc aaccgggcgc cggtcgttgt ggccggcacg gtgaagaccc acgtggatct
    65581 ggacgtgaat gcgccggtgt ccaccttcgt gaaggatccg gaaggcgacc gcaccttctt
    65641 caccctcctc ggctcaaccc atggtacggc gcacctgtcc ggcgacggcc agtccgtggt
    65701 gttcacgccc gaggccggct acatcggcac cgcagacttc accatccgcg ccgacgacgg
    65761 ttttggtcag accatccaga ccatcgccgt caccatcagc aacgcggcgc tgacccgcct
    65821 cgactttgtc gaccgcaacc tgcggctcgc cgtgggcgaa ggtcgccaga tcagcctgat
    65881 cggcgacttc gaagatcagg ccaacgtgga actgccggcg tcctatgtga ccctgatgag
    65941 caccgacggc gccatcggca gcctcgttgc cggcggccgc atcaacgccg tgggcaaggg
    66001 ctccggcgta ctcatcgccg agcgtgccgg tctgcgcgcg gccaccgcgt tcaccgtggg
    66061 tgtgcccacc gacaccgtgc aggcctacct gtacgcgggc ggcatgaccg tctatcccgg
    66121 cgcggtgagc ctcgccgaga acgtgggcaa gcgtcagatc ctggccaatc tgtacggcgc
    66181 catggacctg gctccggcct ccagtggcac gcactactac gtgagcaacg accagatcat
    66241 cagcgtctcg gctgacgggc tgatcagcgc ccgccagagt ggcgaggcga ccgtcacggt
    66301 catcaacggc ggcgccgaga ccatcatccc ggtcaaggtg gagaagccct tcgtcgggcc
    66361 caccgtgctg ggcgatctgg gcggcatcgt gcagggcgcc gacgggtcca tcgtcgcggt
    66421 ggcgccgggc gcgttgcgtg gcaacacgac ggtgtccatc gaagcggtca aggccgccga
    66481 cctgcccgcc ggcatgcccg atggcatggc cctggcaggc gccttcaagc tggatctggg
    66541 cgatcgcaag ctcgatacgc cgatccagct cgccatcccc actaccggca tcgccgcggg
    66601 caccaaggtg gtgttcatgc gctacgccga aattccgatg cccgatggct ccaagcagcc
    66661 catctggctg caggaagaga ccggcgtggt gggcgcggac ggttacgccc gcgccatgtc
    66721 gcccccgtat cccggcgtca cggtgtcggg cacctacacc tgcggcatcg cgccggatgc
    66781 cagcttcagt caggtgcgcg gtcagaccta cgcaaccttc ccggtgggca tcaacgacgg
    66841 cggctcgtcc ttcgccatgc tgtcgtccct gggcggtggc gcggccatgg gggccgcctt
    66901 cggcctgacg gcgggcttca tgcttacctg cctgatcggt tatcacgagc tgcagatcat
    66961 ctcggtgccg gcccagggcc tgccgacggt caccaaggtg ggcgtgcagg tggatccggg
    67021 caaggtcagc gacaaccgca tcgaggtgaa caacccctac agcccgagca cctcgccggc
    67081 caccgagccc ttcgtctcgt cggcccgctt cctcttcaag gacgacggat cgggcaatgt
    67141 ggtgccgttc gtggagatca agggcgaccg cttcacctac gacaatccgg ccgcgccggc
    67201 cttcgccaag aagggcagtc agatcgccga tctcaaggtg gtgttcgaga ccccggacgg
    67261 gcacacgttc gaggtttcgc cgagcgcgac cagcaccgat ggcgacgtct tcgtcgaagt
    67321 gccccagacg gtctccatcg gcaccgccaa gatcttcgtg cggcggccca tctactacgc
    67381 caacggcggc acctgggacg acctggagca actggacagc aacaaggttc agctgtccta
    67441 cgacgcgcgc tacatcttcg gcggcatgtc gggcaatcag gtctttgcca tcgacagtca
    67501 gactcaggat ctggtggcgc gcatcaacgt gagcggcaac ttcccggcgc cccgggccat
    67561 ggcgatcagt tcggacaaca cccgggctta cgtgacactg cgctacaacg gcgcggtggc
    67621 gctggtcgac accatcgccc tgcagcaggt cgatgtggac ggcgatacgg ccaacggcat
    67681 cagcagcatt ccgctgttgc ccggcgccca gcccttctgg atcgccatcg ccaagggcgg
    67741 caagaaggct tacgtgtcgg acgaggtcgc cggtcgcgtg tacgtgatcg acatcgatcc
    67801 gtcgtcctcc acctacaaca ccctggtgaa gaccatcgac gtgaatccgg cgccgtccgg
    67861 tctgcgcggc atggccatca acgccaacgg caagcgtctg ttcgtcgcgg cgccggacaa
    67921 ggccctgttc ggccgccccg gcggcggtga tggcaaggtc ttcgtcatcg acgtggagga
    67981 gggcagcagt acttacggtc aggtggtggc cacgctggac gcaggttccg agccctacgg
    68041 cgtatcggcg accaacgacc cgaacgtgat gatgtacacc aaccgtctgt ccgactccaa
    68101 gggcgtggtg atcgtcaagg gtgacgtggg caattacacc cagaccaccg tgaccctgac
    68161 cctcggtgcc atcacggact ggttcgacgt gaacaacgcc agttccgtgg tcctgtccaa
    68221 ggacaccaag tacgccttcg tcgtcgggta caacacctac atccaggacg tgccgtccca
    68281 cgatccgaac tacgacccga accaccccgc gggtggcaac atcggcatca tccgcgatcc
    68341 cctcgggccc aacccgaccc tcgtcggcgc gacccgcgag atccccatgt cctggcctga
    68401 caacgcggtc ctgtcgccgg acggcaacac cctgtacgcg gccttccgcg gccagcgcgt
    68461 cgtcatggcc ttcgatgtgg ccaagatgat cgccgtcgtc gagagcggca gtcccgatct
    68521 gggcaccacc gcagtggacg acatcgatcc gtccatcgat gtgcgcgcca acttcaagat
    68581 gctcaagaag ggcgggttgt tcaccgatcc ggtgttcggc gtcccgccgg gggccaccaa
    68641 cgggcccatc ggcgtcggct tcaaccaggg gctggtgacc cagggcggcg cttcgctgga
    68701 actgctggcg ccggatgcct ccacgtccaa tctcaacccc aatttctcgt ggatggtgaa
    68761 gggcttcgaa ggggccaagg cgcggctcta cgtgagcacc ttccggggca gcgacggtct
    68821 gttccccacc gatctgggct cgacggacca gaaccccaac cggatcttct cgtccgacct
    68881 gcttgacgcc agctcggcgg attccaccgg cgcgatcttc atcaagcaac tgcccgccaa
    68941 cctcaagctc accgccggtc agcagtatta ctggggggtg gaggtggtgt cgccgtccgg
    69001 caccgtggtg gcccgcgact ggcgcgtgtt caagaccgag gccttcaagg atccgagcaa
    69061 gccctacagc agcgtcaccg tgctgaccca cggcttccag ctctcgccga cccagccggc
    69121 caacctggcc gacgccctgt cgtccttcta ccagatggcg gaaaccatcg ccaaggatgg
    69181 cggtgaaggg gtggcggcgc gctaccagaa gtccaccggg cactggatca tcttctacga
    69241 tggcgccaac agcggtgccc tggtgggcca cgatctggtg gatgccctgc gcatgcccag
    69301 cctgaatggc cggcccattg cgctggtgag cgactggtac gacgaatccg acttccccac
    69361 ggatggcttc agcgaagcgg cggcggatac cttctactcg tccctggctc agctcaacga
    69421 ggaatcgggc ggcaagatct tccagtcgcc catccacctg atcggccaca gccgcggcac
    69481 cgtggtcaat agcgagctgg cccagcgttt tggcgtctat gcgccgacgg tgtccctcga
    69541 catgaccgcg ctggacgtgc atgacttcaa ccagccgtcc ctggacatcc ccatcgcgca
    69601 atacatccag gcggccggtg cagcggcggg cgccgtatcc gggctggtcg gcaacctgat
    69661 ctccctgtgg ggcatgctgc ccaccaacgc cgtcatcccg accggcaact tcctcgaccc
    69721 ggacgtgcag cgctggagca acatcgcgtt tgccgacgcc tactaccagg tggtggccag
    69781 caatacgccg atgggcttcg gacagatcgt caccacgccc aacgggcgct acgtgccgaa
    69841 cttcgactac agcgtcaatc tcaccggccg cgccggtttc acccgggacg acgggctcat
    69901 cagcgcgtcc ctcctggcga gcggcgaatt ccgcgccggt ggtccccaca gtcgtgtgtg
    69961 gcgctggtac ggcggcacca tggacctctc cgccaccacc ttccaggcgg gcggcgagcc
    70021 ggtctaccgg cgtctctacg acaagaccaa gaccgagttc cagggcgtta ccgtgccctg
    70081 gtacaacgcc cagagctttg cccttggcag cggaccgtgg gaaggcatcg gcaccggctg
    70141 ggcctttgcg ccccagggcg gtgtgactta ctccgggccc atgagcgcca ccaaggtctc
    70201 cctggcgacc gacaacactg aggcgcccaa tccttccggc atggccgtac cgagtatctt
    70261 caatggcgat ttcgaggaag gcgccctcaa ccgcagctgg ctgctccagt actcggcggg
    70321 tggcctggcc acgcgcctca ccggcgatct gccgggctgg tccttccatg gcggctcgtt
    70381 tgccaagacc cggacgctga tcgacggcga ccgtctggtc acgctggcgg acggcaccca
    70441 cgccgcaatg ctcgacaacg acaactaccg tatcgagcac aatcgcatgg tggtgcccga
    70501 ctgggccgag ttcctgacgg tggacgccaa ggtgctcacc ggcaaggcgg gcgagaagct
    70561 tctggtgcgc ggctatctgg cggattcgtc ggtaccgatc gagctgggca gcatcgccct
    70621 ggacaacacc ggcaccaact tccagtccta caccttccag gtgcccaccg ccttccgcgg
    70681 cgccggccgc ctgctcgagt tctccatgag cagcggtgcg acggcggatg ccgaggtgct
    70741 tctggatcac gtccaattca ccaagggcct gatcaccgac agcaccctcg atccgtccga
    70801 caaggcggtg ttcttcaccg acaccaccac cggtccgacc acaggcctga gcagtcagtg
    70861 ggtggatctg accaacccgt tcagccaggc tttcaccgtc acggtcacga ccgacccgaa
    70921 cagcttcctg gtggtggccg gcaccagcgg tgatgtggtg ctcaatgaca acaacgccca
    70981 cgatccgctg tacaccgatt acgtcctggc gccgggggcc catctgaagc tcaacctgaa
    71041 gtccaagttc aatgcggact tcatcaagac ctacaaggat gcggacattc ccatccagaa
    71101 gaccaatctg tacttcacca tcaccctcgc caacggcagc atccgcgacg aggcggtgga
    71161 cctgttctac atgccggact acgccgacgg taacgcgacg gatggcctgt ggaccttcat
    71221 ggacacgatg gacggaaaga cccgcaccct gagcttcgac aatgcggccc atgtggagct
    71281 caaggagacg tccgatacct acaatctgtt cggcgtgtcc caatccgggg gcatcgtcca
    71341 gggcctcacc tacactggcc gttatgtgaa cgccttgccc gatttcgcca cctatcaggt
    71401 ggaggagggt gggcgcaagc tggggacgat caaggtcgcc ggctattcgg tgccaaagca
    71461 gaccgtgggt gtgtccctgg caaccctgcg cacgatggtg cagaacatcc gcaccgaggc
    71521 ggagaactac aagaacaacc ccctcaccgc ggtcgtgcat gatccgaccg gctttgctgc
    71581 cggaaagtac aacatcttcc tgaacctgtt cccggatgcg gtgatcaccg acgcgcaatg
    71641 gcaggacgtg gtggatggat tcaacgagtg gtttggcacg ggggccagcg cgatctacca
    71701 gaacaacatc gcccagacgg agttttcgta tggcgagact ggtctcaatc cgatcgtgat
    71761 gatcaaccag ccgtgggtga atacagttgc gaagccagac ccgaatgacg atgcctcgcg
    71821 cagttttgcc ggtctcgact tcgcctacaa caccttcgag tcgaccctca ccaaggcggg
    71881 tgccggggca ggcagcttcg gcggcatggt gatccgcgac gacatctcca tcgcctccaa
    71941 gcgctatctg ctgtcccgcg tggtcaaccc cgagcgcgcg ggtgccatgg gcggtcagag
    72001 cgccatgacg ctggtggtgg atggcatcct gcgcgcctac ctgtcggtcg gcttcacccg
    72061 caaggatatg gggcggtcct acggttgggt ggtaggtcac gaactggccc acaacatggg
    72121 gctgttcgac gagtacatct acagcccctt cacgaacgcc ccacgggatg gtggtgcgaa
    72181 cttcatgtcc aacgccaaca acatccgcgt gagcagttcc caggccgcgg cgctgcatct
    72241 ggccatggac aatcccgaca cgacggtcgc cctgtccagc accgacgcgc tgctgtcctg
    72301 gtacctggac ctggatgcgc tcgacaagtc taatcgtgcg ggacggcaag tcttgaacct
    72361 gtccccgctg gcaggagacg gtgaaggttc caccgacccc ctgggcggtc tgctctactt
    72421 cgtgagtcag aacagtgacg ccacgtcgcc tgtcacgtcc tcgccaattc tgatttccgg
    72481 tgcatcccac gacggcatcg tcaatggcca gttctccgtg gccgatccgg cagcgaccga
    72541 ctacggctgg atcaccgaag gcgatgccaa ggtcagcgct ggcgccggcg tcctcaccga
    72601 agacccgcgc ctcgcctcgc gcttcgccca gaccttcgtg gtcccggcgg atgccgcgac
    72661 gctgcgcttc cagctggtgt ccagccattt cgtcgccaac ggcgacggcc cgccggatgc
    72721 cttcgaggtg gccctgatcg gcgccgacgg caagccggtg ggcggcaccc tgcccctgtc
    72781 cggcagcgac gccctgttca acctgcaggc ggatggcacg gtgtatgcgg ccgaccaggt
    72841 cagcgcggcg ggcctcacca accgtaacgg cggcaccttc ccgtccggcc gtccgatcac
    72901 cgtgagcatc gacctcaccg gcgtcgcggc gggcaccacc gtcaccctgt acttcgacct
    72961 cctgggcttc ggcgaagccg ccagttcggt caccgtcgac aacgtccgcc tgctcaaggt
    73021 gggcgaggtg gaagaccagc cgccggtggc caatgccgat accgccgcgg tggacgagga
    73081 cgcctccgtg ctcgtcaatg tgctcgccaa cgacaccgat ccggaaggca aggccctcac
    73141 cctggtgagt gccgacggtg ccacccacgg ggccgtgacc ctcgaaaacg gccaggtccg
    73201 ttacacgccg agcgccaact acttcggtgc ggacagcttc acctatgtgg tccgtgacga
    73261 ggccggcaat accgccacca cctccgtggc gatcacggtg cgcccggtca atgacgcgcc
    73321 ggtggcgaat ccggacagtg tggcggtgct ttccggcggt tcggtgctca tcgacgtgct
    73381 cgccaacgac accgatatcg acagcccggt gctgaccatc accggcgcca ccggtgccag
    73441 ccacggcacg gtcgccatcg agtccggcaa ggtccgctac acgcccgacg ccggctatgc
    73501 aggcggcgac agcttcacgt acacgatcac cgatggtgac ggcggcaacg ccggcagcag
    73561 cgtgacggtg gacatcctgg tgcacaacac gccgccggtg gccaacgccg acacggccgt
    73621 caccgacgaa gacacgccga tcctcatcga cgccctggcc aacgacaccg acgccgaggg
    73681 tcaggccctc agcctggtga gcgtgggtct ggccacccac gggacggtca gcctcgacgc
    73741 cggccagatc cgctacgtgc cggacgccga ctggttcggt gccgacagct tcacctatgt
    73801 gatgcaggat tccgtggggg cccaggcctc ggccacggtg aatgtcacgg tgaacagcgt
    73861 gaacgatccg cccgtcgcca atcccgattc ggcgaccgtg gaggccggcc agtcggtgct
    73921 catcgacgtg ctcgccaacg atacggacaa ggacagcgcc cagcttgccg tcaccgccgt
    73981 gtcgggcgcc gcccacggca ccgtcgtcat cgagtccggc caggtgcgct acaccgccaa
    74041 cgccggcttc accggcgccg acagctttac ctacaccgtc agcgacggtg acggggccag
    74101 cgtcgacggc agcgtggcga tcaccgtcca gctccacaac accgcaccgg tggcccatgc
    74161 cgacagcgct gtaaccgacg aggatcattc ggtcctcatc gacgtgctgg gcaacgactc
    74221 cgacgcggaa gggcaggtcc tgagccttgt cagcgtgacc ggtgccaccc atggcacgct
    74281 ggccatcgaa ggcaaccagg tccgctacac gccggacgcc gactggaacg gtgccgacag
    74341 cttcacttac gtggtggcgg acagcctcgg cgccaccgcc aagggcgccg tggatgtgac
    74401 ggtgaccccg gtcaacgacg cgccgaccct ggatgccctg gccgacgcag ccgtcaagga
    74461 aggggacagc ttcaccgtca ccgccgtcgg tcatgacgtg gatgccggcg atgtcctgaa
    74521 ctacgccctc gacgccgccc cgcagggcgc aaagatcgac ccgctcaccg gtgccctcac
    74581 ctggaaggcg accgacggcg acgccgccta cgccttcacg gtccgcgtca gcgacctctc
    74641 gggcggcacc acgacccgca gcttcaccgt caatgtggcc aatgtcgcgc ccaccctgca
    74701 ggcgggcggc ctgcccgccg tgtatgcggg tcagagctac accctggagc tctccagcag
    74761 cgatccgggt gacgacaccc tcagcctgtg gcacatcaac tggggcgacg gccaggtgca
    74821 ggatgtggcc ggcaatccgg gccagatcac ccacacctac gacggcagcg tgctcggtca
    74881 ggtacagatc cgcgccaccg ccaccgacga ggacggcagc tacgcccttg acccgctggc
    74941 catcgcggtg ctgccggtgc ccctgcaggt gcgcagcttc acgtctgaca gcaacggctt
    75001 cgcggtgcgc ttcaacgacc cgttcgacgc ctcggtgatc aacctctacg actccaccct
    75061 gagcaacctg ggcgtgtccg acgtggtcct gagcggcagc ggcggcagcc tggtcaaggg
    75121 ctccctggtg ttcgacgccg acggcaaggg cctgcgcttc cttgccaccg gcagcggcct
    75181 cgccgccgac acctacagcc tcgtgctgaa gagcggtccg ctggccttcc acagcgcctt
    75241 cggcgccctg gacggcaatg gcgacggcac cggcggcgac gactacacca cgcgcttcac
    75301 catcggtccc gtgccggccc tgcgcctgtc cctgccggac ttcgtccgcg ggccgggcca
    75361 gagcgtggat gtgcccgcca ccggcaagta cctgccgctg agcctcagca gtgctggcga
    75421 cgtgcgcaac ctgagcttca ccgtacgctt cgatccgagc ctgctggcca tcaccggcgc
    75481 cgtggccggc gccgggctgc cgtccggcgc gagcctcaat gtggatacct cggtggccgg
    75541 ccagatcacc gtgaccatcg cctcggcgac gcccatcgcc gccggcaagc tcacgctggt
    75601 gaacctggtg gccagggtgc ccgacacggc ggcctacggt gcggtggagg tggtggacat
    75661 cggcaacgtg tttgccaacc agacggccgc cgaccgggct gacgacgacg ccctgcacgt
    75721 ggtcggctac atcggcgacg gcaaccgcaa cgccaagctc gacaaggacg acgtcacgct
    75781 gctgcagcgc aactcgctca agctcgacag cgggctcgct gcgtggtcct acatcgatcc
    75841 gctgctgatc ggcgacatcg acggcgacgg caagctcacc actgccgacg cttcccgcgt
    75901 cgcccagaag atcgcgggtg tggcgcggcc ggaaatcccg gacgtcccgg ctggcctcaa
    75961 ggtgaacttc gccaccgcac ccacaccgcc gaccgcgccg gtgatcgatt tcggcagcag
    76021 cttcgccggc ttcaccgtgg gctcgagcga tccgaagttc aagaaggaga actggaagaa
    76081 ggccttcgtg acgaatatgg cgaccagctc caccaatccg aacgcgaacc tgaaagtaac
    76141 ggtagacgct tcggtacaat ccaccacgcc cgcctgatgg gcgtggtgag aacccctgga
    76201 caatcggaga ttcaacgtgt cagacaacgc agcagtaccc gagaccccgg aaggccagcg
    76261 catccgcatg aacacggcgt ccctcaagag cacctactgc aacatggtca acgcgaccac
    76321 cacgcgggag gaggtcgtgc tcaacttcgg cgtcaatgaa agctgggacc gcaccggcgg
    76381 cgactacgag gtcaagctgg agcatcgcgt catcctcagc cccttcgcgg cgaagcgtct
    76441 gtcccagatg ctcagcaagc tcgtggacga atacgagcac cgctacggcg aactgaagta
    76501 attctccctc cggccggtca cgggcagggc gccatcggcg cacctgccgg tgccggctgt
    76561 ccaagttttc cgaacggaat cttcacaccg tgcacgctgc gtcccgctcc gtgtccgctg
    76621 ccccggcgga cgccgccgtc cctgtcgcgc ccgacgccct gtggggcgcc tttgtcagcg
    76681 ccgacaacgc ggccgatttc tgcaagagct ggctggccct gcagtgcagc caggtggggg
    76741 acgtgcgcgc cggcctgctg ctgctggagc agggaggcgg caccttcgtc ccggccgggg
    76801 tgtggcccac cgaccagacc gacgtcacct acctcgcgcc gggcgcccag cgttgcctga
    76861 gcgaacgccg cggcaccctg cagcggggcc tggaaggggc gctggtggtg gtcgcttacc
    76921 cgctggaggt ggatggccgc atccacggcg ccgtggtgct ggacgtggcc gagcgcccgg
    76981 acgcggccct gcaggatgtc ctgcggcgga tccactgggg cgccggctgg ctcgagagcg
    77041 tgttcctgcg ccgccaggcc caggacgaag ccctcgccct gacgcgcgcc cgtgcggcgc
    77101 tggacgtgct gggcgtggcg gccgagcagg cgcgcctgga agacctggcc ctggcggttg
    77161 ccaacgatct ggccagccgc tttgcctgcg accgggtcgc cttcggcgtg gatcgcgccg
    77221 gacgcgcccg cctggtggcc ctgtcccact ccgccttctt cgagaagaag agccagttcg
    77281 tggtggccct cgagaacgcc atggacgagg ccctcgacca gcgccgcagc gtgatctggc
    77341 cgaccgacac cgacgccgcg gcgggcggca ttgccatcgc ccaccgggac ttcgcggccc
    77401 agcgggccgt gtgctcggtg ctcctcagca gccgcggcca gggcatcggc gtgctgacct
    77461 tcgaacgcaa tgccggcgag cccttcgaac ccgacgaggt cctcggcttc gaagccatcg
    77521 gggcgctcct cgggcccctg gtggagagcc gcatcgagct ccatcgctgg ttcgccgggc
    77581 ggctcgccga caaggtccgg gacggctggc ggcatctgcg cgatccgcgc cggccggcct
    77641 tccgtgccgc ggtcaccgtg atcgccgtgc tgctggcggc ggccgtcctg attgacggcg
    77701 attaccgggt gagcgccaag tcggtggtgg aaggcgaagt gcagcgctcc gtggtggcgc
    77761 ccttcgaggg ctacatccgc gaggcgccga tccgcgccgg catgctggtc aagaagggtc
    77821 agatgctggc ccgcctggat gatcgcgacc tcctcatcga acgccagcgc tacagcgccg
    77881 agcgggagca gcacgaacgc cgctaccggg atgccttgtc caagcacgag cgcgccaacg
    77941 cccatgtgtc cctcgcccag atggccgagg ccgaatccca gctcgccctg gtggaagaaa
    78001 agctcgcccg cgccgaggtg gtggcgccct tcgacggcgt ggttgtcacc ggcgacctga
    78061 cccagctcct cggctcgccg gtggagcagg gcaagctgct cttcgaagtc gcgcccctgg
    78121 acgcctaccg ggtgatcctc aaggtggacg accgggacat ccgttacgtg cgcgccggcc
    78181 agggcggtcg cctggtgctc accggctttg ccggcgacgc cctggacttc aaggtgcaca
    78241 acatctccat ggcccaggcg gaggagggca agaacctgtt ccgcgtggag gcccgcctcg
    78301 accggggcga catccccctg cggccgggca tggagggcgt cggcaagatc gtcgtcggcg
    78361 agcgcagcta cgcctggatc tggacccacc gcctgttcga ctggctgcgc ctgcaggcct
    78421 ggaactggct gccttaaggc acacacgccg tggccggctc cttcctcagc gcctcctggt
    78481 accgcgtcgc cggcctgcgc ccgcagctgc gcggccacgc ccaggtgcag cgccagcgct
    78541 tccgcggcaa accgtggtac gtgatgcgcg acgccgcgtc gggcaagatc caccgcttca
    78601 cgcccggtgc ctacgcggtg ctccagcgca tggacggccg aagcacgctg gacgtcatct
    78661 ggcaggacgt ggcggctgca gccggtgtgg atgcgcccac ccaggacgag gtgatcgaac
    78721 tcctctccaa gctccacagc ggcgatctgc tccagaccga tctgccgccg gacgtggccg
    78781 agctggccga gcggggcgcc cgccagaagc ggcagaagcg cctgcagagc ttcctgaatc
    78841 ccctgtcgat ccgcctgccc ctgtgggatc cggacccctt cctcaaccgc agctggccct
    78901 gggtggcgcg cctgttcggg cctctcgggg tgatcctgtg gctgctcgcc gtggtaccgg
    78961 cgggcgtgct ggtgggcatg cactgggacg aactcaccgg caacctgggc gaccgcctcc
    79021 tcgccaccga caacctggtg ctgctgttct tcatctaccc ggcgatcaag tttctccatg
    79081 agctgggcca cgcctgggcg gtgaaatccg gccacggtga agtgcacgag atgggcgtga
    79141 tgttcctggt cttcgcgccg gtgccttacg tggatgccac cgcctccggc gccttccgca
    79201 gcaagtggcg gcgcgccctg gtgggcgcgg cgggcatgct ggtggagctc tttctggccg
    79261 ccctggcggt gatgctgtgg gtcctcgcgg agcccggctt cgtgcgctcg gtggcctaca
    79321 acgtgatctt cgtggccggg ctgtccaccc tggtgttcaa cggcaacccg ctgctgcgct
    79381 acgacggcta ttacatcctg gcggacctca tcgagatccc caacctggcc cagcgcgcca
    79441 accagtactg gcagtggctg gccaagcgcc acctgttcgg cgcggtggag gtggagcgcc
    79501 ccgcggccac gccgggcgag cggcgctggt tcctgttcta cggcccggcc tcggccctgt
    79561 accggctgtt cgtgacgatc tccatcaccc ttttcatcgc cggcgagttc ttcttcatcg
    79621 gcgtggtgat cgcgatctgg ggcgccatca ccatgttcgg cgtgccggtg ggcaagatgc
    79681 tgggctatgt gtggggcagt gccgagctcc agcgccaccg gccgcgtgcc ctgtgggtca
    79741 cctttggcgg gctggcggcg ctgctgctct ttctcttcgt tgtgccgatg cccctgcgca
    79801 cccacgccga gggcatcgtc tgggtaccgg cccaggccga gctgcgggcc aaggggaatg
    79861 gattcgtgga gcgcgtgctg gccgcgccgg acgcctgggt gcaggccggc gatctggtca
    79921 tcgtcacccg ccagccggat gtggatgccg acgtgaccca gcgccgcgaa cgggtgcgcc
    79981 agctggaggt gcagtacgcc gcccagatgt tcgacgaccg cctgcaggcc gccgtggtgg
    80041 ccgaggatct gggccgcgag cgcgccgccc tggcccgcgc cgaggagcgc ctggacgagc
    80101 tgctgctcac cgccggcgtg tccggccgcc tgcgcctgtc ccgcccggac gatctgcccg
    80161 agcacttcgt caagaagggc gagctggtcg gctacctgct ggcgggcccg ccgcgggtgg
    80221 tacgcgcggt ggtcacccag gacgacatcg ccctggtgcg ggatcgcctg gaggcggtgg
    80281 aggtgaagat cgccgaccgt cttgccgaga cctatccggc ccaggtgagt cgtgaggtgc
    80341 ccggcgctca ggatcagctc cccaatcgcg ccctggccat ggaaggcggc ggcctgcacg
    80401 ccaccgatcc ccgcgatccg gatggcctca agaccctgga gcggctcttc cagttcgatc
    80461 tgaccctgcc cgacagcgtt ggcgaggtgc agcttggcac ccgcgtctac gtgcgcttca
    80521 tccaccgggc cgagcccctg gccgatcagt ggggccggcg cctgcggcaa ctcttcctgt
    80581 cacgcttcca tgtctagggc cgtgctgtcc acctggggcg tccatgcctt cgcccccggg
    80641 ctgtatcccg aacggcagga tgccaagcct ggcaagctgg accgcttcat gggcggtctg
    80701 ctcggccgct gggcgcgcca cggttctgcc ttgcgcctgc cgccggtgcg ccgttttgcc
    80761 gaacaggtga tcgcccacga aggcgaactc gctgccctgg acgaagccac cctgcaagcc
    80821 cgcgtcgcgc ccctgcgcgc cgctctcgcg gccagcgggc tcgacgacgc caacgcggcg
    80881 gaggtctttg ccctcgtgcg cgaggtgtgt ttccgtcgcc tgggcaagcg ccactacccg
    80941 gtgcagctga tgggcggcta caccatgctc aatggcggcc tggccgagat gatgaccggc
    81001 gagggcaaaa ccctcaccgc ggtgctgccc gcggtggcgg tggccctggc cggcgtgccg
    81061 gtgcatgtga tcaccgtcaa tgaatacctc gcccagcggg attccgagca gctcgcgccg
    81121 gtgtatggct tcttcggcct cagcgtcggg ctggtcacgc cggagcagga gccgcccgag
    81181 cgcatcgtcg cctacggctg cgacgtgtgc tactgcgtga acaaggatct ggtcttcgac
    81241 tacctgcgcg accgcatcac cgcccagcgg atcaaggcgg gtgcgcgggc gctgctggaa
    81301 ggctggctcg gccgcagcgc gccggtgggg ggctcgggga ccctgctgcg cgggctgtac
    81361 tacgccatcg tggacgaggc tgacagcgtg ctcatcgacg aggcgcgcac gccgctgatc
    81421 atttccgccg agcaggacga ccccaacggt cgcgccttct acgagcaggg cctggccgtg
    81481 gcccgcagcc tcaacgccga cgagcactac cgcctgcacc tcaaggaacg cagcatcgaa
    81541 ctcaccgaag ccggccgcgc cgccgtggcc gaggcgtccc gcggcctgcc cggcctgtgg
    81601 caggtgtcgc gcgcgcggga ggaactggtg gagcagggcc tcaccgccga acacctcttt
    81661 gaactggatc gccactacat cctggccgag ggcaaggtgc agatcgtgga cgagtacacc
    81721 ggccgcgtca tggccgaccg ctcctgggaa aaaggtctgc accagatgat cgaggtgaag
    81781 gagggcctgg agctcaccaa gcgccgcgac accatctcgc gcatcaccta ccagcgtttc
    81841 ttccgccgct acctgcgcct ggccggcatg agcggcaccg tcaccgaggt ggcgccggag
    81901 atgcgcaccg tgtacgccct ggacgtgctg cgcatcccgc ccaaccggcc agtgcagcgc
    81961 cgtgacctgg gcctgcgcgt gttcgcccgc agcgaggcgc gctgggatgc ggtgctggct
    82021 tccgcgcgcg cccacgccgc cgctggccgc gccgtgctga tcggcacccg ctcggtggcc
    82081 gcgtcggagc tcatcggcgc gcgtctggcc gcggccggtc tggatcacga agtgctcaac
    82141 gcccgccagg atgcccgcga ggcggagatc gtcgccgacg ccggccagcc cggccgcatc
    82201 accgtggcca ccaacatggc cgggcgcggt accgacatcc tgctgacgcc cgaggtcaag
    82261 gctgctggcg ggctgcacgt gatccttacc gaataccacg actcggcgcg catcgaccgg
    82321 cagctctacg gccgctccgg gcgccagggc gaccccggct cctacgaggc cctggtgagc
    82381 ctggaggacg agctcttcct tgcccacgct gcccgcctgg tggcctggct gcgcgtccgc
    82441 acggcgccgg acggggaggt ggcgcgccgc ttcgggccgc tcctgcgccg cttcgcccag
    82501 ggcgccgccg aagcccagaa cgcccgcatc cgccgtcaca cggtggattc cgacaaccgc
    82561 atggaacaag cgctcgcatt tgccgggcga ggggaatgag catgaaacgc cgcaccaccg
    82621 ccggggtcgt cctggccgtc ctgaacacct tgggtgccgt ctccgcggcc cgcgcgggcg
    82681 agttcgactg catgatcgag ccgcgcaaga cggtggacgt gcgcgccgcc agcgaagggc
    82741 tcatcgaaaa gatctgggtg gatcggggcg acatggtcaa ggccggccag gtgctggtca
    82801 ccctggattc gggcgtcgag aaagccgcca ccgaatccgc ccgctaccgc gccacgatgg
    82861 aaggcaagat ccgcaccggc gagagccgcg tcgagttcgc cacccagaag tacaaccgcc
    82921 gcgacaagct ggccggccag tcctttgtct ccatgcagga ccgggacgaa tccctcaccg
    82981 agaagcgcct cgccgaatcc gagctgatcg aggcccgcga cgaccgcaag ctggccgaga
    83041 tcgaataccg ccgcctgtcc gagcaactgc gcctgcgcac catcaagagc ccggtggacg
    83101 gcgtggtggt ggatcgcctg ctgcacgccg gcgagctcgc cgacaaccgc gacctgcgca
    83161 agcccatcct caagctcgcc gacatcggca ccctgcacgt ggaggccctg ctgccggtgg
    83221 acgcgtgggg caagctggcc gtcggccaga gcatcgagat cctgccggag agccccgtcg
    83281 gcggccgcta caccgcgacg gtcaaggtca tcgaccgggt catggacgcc gccagcggca
    83341 ccttcggcgt gcgcatggaa ctccccaacc ccggcctcaa gctgcccgcc ggcatccgct
    83401 gcaaggccag cgtgccgggg gtgacggggc gaacggtgct cgcggcgccg ccgaagaagc
    83461 cggcggcggc accgactgcg ccgggcgccc cggccagcca ctgagcccgc gggccccgcg
    83521 ccgcagcggc tttgtcagcg ggatgacttc agaagcgccg gccgatcatc acgaagaggc
    83581 ggggattgcg atccacgtcc tcactggtgg gggcgccgcc ctgagtgcgc caggccaggg
    83641 tggcgtccag gctccagccg tccctgaggg aacgcacacc cacaccggag ccggccagcc
    83701 ggcgcgtgga ggccgaattg acgtcccagg gcttggcgtt ggtttccacg tggcccgcat
    83761 cgtagaaggc gaagggggtc acgacgccca cgtcgtagcg gatttccagc tgtgtgaacc
    83821 agccctgatc gccgacgcct tcgcccagcg ggaaggcgcg caccccatag aagccgccca
    83881 ggttgaattt ttccgacgaa tccaggttct tgtccgccca ttgaccggaa tagcgcgcgt
    83941 aggcgtggac gctggaggtg agctgctgga tgcgcgcgac atcgagattc agcttgttga
    84001 agtggccggc cgtccgggcg gtgacccggt ccagggcatc catgcccgga tcgaggctca
    84061 gggagccggg cagccaggtc agcgagccat acgtcacgcc gccaccgagc agggtgtcgc
    84121 gccggtcgaa ctggatcgcg accggcgtgc cgtcgctgtg cttgttgcgg atcaggccgg
    84181 tgctgtcgta ccgatcctcc aggtttttgt gctgatagcc tgcggacagc agcatattgc
    84241 ttgcctggga gcggatcagc ggatagctca ggcgggccgt ggtgatcttg gcgtagccct
    84301 tggcatccag ggcggcgaac tgggcaccga gctggtagtt ggtgtaggcg tagcccacct
    84361 gtccgcgcag cccggatgcg ccaatgggca tctcgtagtc gagggacccg agcagcatgc
    84421 tctggtcggt tgccagggcg ttgatggaaa tcttgtcgcc gtaacggaag ggactgttga
    84481 tgaacacggc gccgcgagcc cggttctggc cggtggagcg ggcgccagtg ttgtccaggc
    84541 cgacctcgcc gcccacaagg gagacccgct ccacgttgac tgacaagtcg gcctcgccgg
    84601 tccggtcgcc cggctggatc accggacgga tcttcattcc cggctgatcg tcgaggatca
    84661 gcagggtgcg ttccaggcgg gcgttttcga tggcatcgcc cgaccgcagc ccgaaatcca
    84721 ggaacggctg ggcggaggca ggcagcgggt cggtgcccgt ggcctggatt ctgccgtaac
    84781 ggccttccac cacttggata cgcagcgtgc cgtccttcag gtcctggggc ggcagcacgg
    84841 cctgtgtgaa gggatagccg gcagcgcgat actggcgctc gattcccgca gccagggctt
    84901 cgagcccggc cagatcgaag gactgcccgc tgaccgggcc cagcccggcc tgcagcgtgg
    84961 tgctgtcgag gagggtattg ccggacaggg tgatgtcccg gagcgtgacg cgggggccgg
    85021 ctggtgttgc cacggcaggg gatgcaggcg ggcgaaccgt caggtcggtg gcgggggcgg
    85081 cgggcacctg gggcggcagc gggccctgca gggcgccgcc ctgcgcgaag gcctgcagcg
    85141 cgcagcccgt cagcgccaga tacgttgcga cgatcttctt gttcatggac gtcctgtcag
    85201 ttggtcaggg actggagtgt ggcggagcgg cccggattga tgcccgagtc gatgacgaag
    85261 acgtcgagcg ggcccgagga gctgggcacg gtcacgttgt tgacgttgag ttcctgggct
    85321 gccttgcgct tgccggcttc cgtgccggcg gcggcttcgc ccgaggcgct gcttgagaca
    85381 taggtgacgg tcgtgcgctc gccaaggggc acctcgtttt cagcggcggt tggcgcgctg
    85441 cgttcctggg gcggtcggtc ggccgcatcg gttcgggcgt cgctcctcgc cggggaccgt
    85501 gcgtctggcg catcgccggc aacgaaattg agggtcgagc gtccagcggc gggcgtttca
    85561 ttggccgagc ctgccgcgac gcgctgatcc ggaccggcgc cgccggccgc cgtgccgccc
    85621 gccgggtctg caggggccga acccggattg gccggggtgc ccgagacaaa gttcagcgtg
    85681 gacggggtgg ccccgcccgg tgcggcgccg ttcgcgcggg gggcggcagc ctgaccgccg
    85741 gccggcacgt aattgagccc gccgaagctg gccggcggag tgccggtggc gagcgcggaa
    85801 gtgccgttgt cgatgaatgc cggaacgatg atgggtgccg gtggtggcgg cggcgccgtg
    85861 ggcgggacga tcgtcagcat gccgtcgaag gcggtgattg cgtagttgcc gttgtggagg
    85921 ctggcggacg tgattgcgta ggtgcccggc gcttcaccgc tgtcccgcag caggctgccc
    85981 aggctgtccc ctgcgagcag cgtgccctgc gtgatccggt aggtgagggc cggatcggtc
    86041 tccccgaagg tcttctcctg attgtcggcc gcgacggtga tggcacgggg cgcgaggctg
    86101 gccgtattgg tcgcccccga accatcgagg gccgacccgg cggtnnnnnn nnnnnnnnnn
    86161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    86221 nnnnnnnnnn nnnnnnnnnn nnnncgctcc cgttgtaggc cgggttgatc tggtagttgg
    86281 caaggttcgc ggtgccgcct gagggcgtca gtgccgtgac gaagttgctg ccattggcga
    86341 cgacattggg gctgttgagg ctggctgctg caagtccggt cagggtgtcg ctgccgagca
    86401 ggccataggt gctgaaggag gtcggaacga tgctggtcgt gccgttgtag gtgccggaaa
    86461 cggtgatgcc cagcggcgcg cggcccagac tggcgacatt ggtggtcacg ctggtggcgt
    86521 ttccaccggc gtcgacgacg gcgctgtagg ccggggcgag ggcgtaattg cgaatgtctg
    86581 cggtgccgct gccgacgacc accgagcgca cgaaattgct gccgttggcc gatacgttgg
    86641 agttgtcgat cgtggcgccg gagagttgcg tgatcgtgtc gctgccgagc aggccgtggg
    86701 tgctgaagga tgtgggcacg atggaggtgc tgccactgta ggtgccgcca acggtcacgc
    86761 ccagcggcgc tgccgacatg gacacgccgt tggttgcctg ggacccgtcg gcggcgctgc
    86821 ccggtgtcgg cgtggcggtg ccatacgtgc tgccgttcag gacatagttt cgggcgtcga
    86881 taccgccgtt gagggtgacg gaggtgacct tgccggcgga aacgaggttg gggctgtcga
    86941 cggaaaagcc gccgagagtc tgtccattga ccaggccgga cacgctcacg cttccacccg
    87001 tggcgagggt gccggtgccg ccgtcggcca cgccggagac cgcggtgaac tgggcggtgg
    87061 cgttgtaggt cggggtggcg tagacgccga gcggcttcgg cgagatgctg acggtgttgt
    87121 tgagcgtggt gatcgcgccg ctgctctggg cgacgtggta ggcgggcgtg cccccgttgt
    87181 acagggcgta gttgccggcc aggaatccgt tgctgccgct gacgcccgtc acgtacttgc
    87241 ctgccgtgat ggcgtccacg ctggcgtcgg agacggtcac gccggtgagc cgctcgctgc
    87301 cagccagtcc gctgacggtc agggtgccgc cggagttgat ggtgccggcg ctggcattgg
    87361 tggcgccgac gctgctggtg aactgggtcg tcccgttgta gagggcgttg agggccacgc
    87421 cgaggggcgc ccggttgatc acggcggaaa cggcgccgcc ggagagggtg taattggtag
    87481 agttgctgcc ggtcagggcc aggctggagg cggcccactg ggtgtaggtg cccacgtcct
    87541 tgctggccac gtcggcgctg ccggcgagat ggacatcgtc gccattgacg acgccggagc
    87601 cgagattgag ctggttggtt ccgaaggtcg tggtgccgtc atagttcttg ctgccgttca
    87661 cgcccagaac cgtcaggctg aacgcgttga tcgttgcggt gttctgggcg ctgcggctgc
    87721 tcagggcggg cagcgtgtag ttgctcgcca gtcccggcgt gccggaggat ccgttggcca
    87781 gggtcatgcc gcccagcgag gtcagatagt tgatggcgtt gtcagccacc gccgacgacg
    87841 tggctgtggc ggggccactg ccggagagcg acagcgtttc gccattgacg ccggatgccg
    87901 tgatctgggc gttgctgaag ctggcgtcac cgttgtaggt cttgacggca ctcagggatg
    87961 ccgtggcagg ggtgacggtg taggcgggcg agacagcgaa gttgtagccc agcacgtagt
    88021 tgttgccgtt ggccgagtag ttccgtgaca gggcgctggc gctcaggttg gtgtaggcgc
    88081 caacgtttgc actgggcgtg acgtaggtgg cagaggtgac attgccgctc gaatagcccg
    88141 agccgtcggg gtccatcacc gtcacgttgc tgaagctcag gttggaggcg gtgagggtgc
    88201 cgtcatacac cttggtgccg ctgagcgtgg ccgacacgta gatcggcagg gtcgccatgt
    88261 tgacggcgtt gtaggtggcg tacaggtagg aattgttcgc gctggcattg accgacagca
    88321 gaccgcagtt gggatagccg gtacggcagc cggaggtcgg cgggctgtag gtgaagctca
    88381 cacccttcag gtcgccgttc aggatgaaga tcagatcggc gacggagtag gcgttgaagt
    88441 agatgttgcc ggccagcccg ttggtggcca tgttgagggc gtaggacccc ggggtgtcgt
    88501 aggtgctgct cccttcgatt cggatgctgc cggtggtggt ggaggcggcc agggtcgtct
    88561 gcccggcgtt gccgctgggg ttgtcgatct tcaccccgaa gaccttgtcg gcgttggtcc
    88621 cgacaccccc ggtgtatttg cccgagatgt tgatgaagcc gccggacgat gcatcggtgg
    88681 cacccggagc gtagtagccg ctgtcgatga cggcgctggc gccaatgtgg acggcggtgg
    88741 tgtcgctgct gccgccgcct cgctgggtgg aattaccgtt gatcgtgatg ttgccgccgc
    88801 gggagagaat gttcgccgac gagccgatcg acaccgcctc ctggctgctg gaatagttga
    88861 gggcgtagcc gatgccatgg gagggggtct gggcgccgct cacgctgccg ccagcgccgc
    88921 cgatgaggat gtccccgccg ttggagttga gggtctgact gctggcgacg cgcacgccgc
    88981 ccagggtgcc ggcggcggcg ttggccgcgc tcagcgtgat gttcaggggg ccgccggtgg
    89041 acgtgatcga actgttgacg gcgatgttgc gttcggccag aaaggtcagg gacgtggtcc
    89101 ccgaggccgc cgtcttggtg atggtgtcgt tgatgaagat gtcgccgctg ccacccgccg
    89161 tgccgtagct cgcggtggag atggtgacat tgttgctgtt gatcaggctg gtgaaggacg
    89221 cgccggtggc gttgcccccg ctcgctgcca cggtgtagtt attggggtcc accagcaggg
    89281 tgccggtggc gccgtggggg gccagcgtgg tcaccgccag ggtgtctgcc atgttgacgg
    89341 tggcggcgct ggtctcgatg aatccgccgt ccccgccctg cggggcgctg gcgtccaggg
    89401 tgccgcctgc gttcagcgtg ccgtgggcca gatcgccgat cagccggatc tcaccccgct
    89461 cgccggtcgc caggctgcgg gcgcggacca gaccggtgtt gttgacggtg gcggtggtca
    89521 gttgatcggc ggccctggcg gtgagataga tgaagccgcc atcggccacg atggcgccgc
    89581 cgttgctcac cagcgcatcc agcgccccct gttccacctg aagcttgagc ggaccgccca
    89641 gatccagcgt gacacgcttg cccgccgcca ggagcacgtt gccggcgggc gcattcacgc
    89701 tgccttcgtt gctcacccgt gcagcgatca gggccaccgt cccgccctgg ccagcgtcac
    89761 ctgctgcggt gatgcggccc tggttgatca ctgcgttgct gccagcaccg tcgagcacat
    89821 agcggcccgc caggaaatca cttgtgctca ggtcccgtgt cgacgccacc aggctgccca
    89881 catccacctg ggcagtgggc gaaaagagca cgccattcgg gttgatgagg aagacctgcc
    89941 cgttggcctt cagggctccc tggatcagcg agacatcgga gcccagcaca cgattcaggg
    90001 cgaccgcacg ggtgccgggc tgtacgaagt tgaccgtgtt gtcccgtccg atcgaaaagc
    90061 cctgccagtc aatggccagc ttgttcgacg tctggtggat ggtcaggctg ttgccgttct
    90121 ggctgatgct gccggtgccg gcggcaatgc tgccgcctgt cggcaacggg cccgcggccg
    90181 cggccagccc ggccccgagc gacgcgagga ccgcaaccga ccccacgcgg gcatgccggg
    90241 tcttgcccgc actgcgtgcg cactcggcca ccacctgcca gatgttcagc gcacgattcc
    90301 atacaacacg atagatatga ttcacggagg tcctcgcaag ggctgggtcg ctgccagagc
    90361 cacatgtcga accagcccgg ctgccggaac cgggcgaggc ggcggtcgcc gcccttggga
    90421 cgcatgaagg cttgactgat caaggccggt tatcggcagt caccgcgaag gcttta
//
LOCUS       DAOQMM010000004        79387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_37_length_79387_cov_51.747618, whole genome shotgun sequence.
ACCESSION   DAOQMM010000004 DAOQMM010000000
VERSION     DAOQMM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 79387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 79387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79387
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_79387_cov_51.747618"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..237)
                     /locus_tag="PLH85_01220"
     CDS             complement(28..237)
                     /locus_tag="PLH85_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63204.1"
                     /translation="MNFLVYLIPHYKIRLRLICSQAPIDGGEGCEVVVDGSVQVPIDE
                     CAAQRAFWDWVFALKSQLRRGARAG"
     gene            629..1858
                     /locus_tag="PLH85_01225"
     CDS             629..1858
                     /locus_tag="PLH85_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB63205.1"
                     /translation="MNALPSVSRPFSDRAETVALCLIVVCAFVVPLPTALISICTFLF
                     LLAWLAAGRLGERWATLHRHPQGPLCLALLTWMGVSIAWSPAPFHEALDNWWHFREYI
                     LFLLMLTVVAREPKKWERRILLGYLAGYCVSLLTSYLRWFEVIPLYDQVGEFAGFGGH
                     TGFSLMLAITSYACIWLWRARPGLRVFWLLLMILSLFNLYFINTGRTGQVAFLALIPL
                     IVFRWMGLPGLIAGALGAVAFAGSLYFLSPTVHYRVNNAVYQIEQYRAGNTTTSDGLR
                     IEFWVNAVKLIRHHPILGGGSGSFAAEYEVLAAEQHMTGDHVAVNPHNEYLLMWAQYG
                     LPGLGLFLALWLSQWRRACREAETRRYLFHGLILAMAVGDLFNSLILDNMEGHFYMLV
                     SVALGAAWARDDRAGGR"
     gene            1935..2861
                     /gene="ybiB"
                     /locus_tag="PLH85_01230"
     CDS             1935..2861
                     /gene="ybiB"
                     /locus_tag="PLH85_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HNB63206.1"
                     /translation="MSYAPYIKEIGRGPKGSRPLTVDQAEQLFGDMLDGRVPDLELGA
                     IILSMRIKAESVDELFGFQRALDARTAHLAVPPGPRLVVLPTYNGARRQANLMPLLAR
                     LLAREGVPVLIQGRHDFESRVSPFELLAALDIHPATSLSDATDRLARDRLACVPLDLL
                     APGLVPLLATRVRLGVRNSAHTLAKLLDPAPGHSVRVASMTHPEYLERIHAHLVATGA
                     TAMLLRGTEGEAFANPRRRPLLERFANGVADVAFPAEEGGAPPLPGVTDNPEVAANAE
                     LIRAMLAGAVPIPSPLLDQVAALRDLAHLPRA"
     gene            2990..3787
                     /locus_tag="PLH85_01235"
     CDS             2990..3787
                     /locus_tag="PLH85_01235"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB63207.1"
                     /translation="MSKKALCIGINDYPGTGSDLSGCVNDANDWATTLTGRGFTVTKL
                     LDAQATKAAMVSAMSSLIAGAEPNSTVVITYSGHGTWVPDQNGDEADGRDEGLCPWDI
                     AQGKVLIDDEIAILFAKRAKGVRVLLIADSCHSGSVTRAPEDIPEPGEPKPRFLPLSL
                     WLKTGYNPPVVPPKPPAGTLGLDRAGGDTLLAGCKDEEFSWDTSFAGRPNGAFTYYAL
                     KALGSLPANATYADWFKKIRTYLPSSACPQNPQIFGPDGADAFPIFS"
     gene            3851..9313
                     /locus_tag="PLH85_01240"
     CDS             3851..9313
                     /locus_tag="PLH85_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB63208.1"
                     /translation="MPRTYRIRGAQDASRPAVALPIADTAAISVTHVGHLLPGTARDA
                     APNELDIPADTVVRVTLDNGFVLWSRADDLIREYGRKALARDGGEGWEFGPLVPPRRG
                     AGERGLLGLGIKVLDFFGIHVMNKPAAAIAAWLEDKRLGGHAPGVYRCPLDGPFALSP
                     LGTKETLAAAPGPSLVFLHGTGSSTQGSFSELWDPKNASGAVLRARLKGVYGDRVYAL
                     EHRSLTESPIQNALQLVERLPESTELHLVSHSRGGLIGELLCLSGVDALAESLSPTTL
                     DALFNPDRIIARQLGLGPLGEQALADRKAAYDAERRRLSDLVGLLAARKIRVSRFVRV
                     ACPARGTTLASGRLDRWLSTLDFLAGRVLGDGLFIGGLDFVLAVVHERTDPRTLPGLE
                     AMMPGSALTSLLAAPGLTTRADLTVIAGDIEGDSLLQNLKLMAVDWFYAADHDLVVNT
                     GSMSGGLKRPPGGARYRLDRGSDVNHFAYFQNKKSVEWLLAGLTRADGADGGFLTIAS
                     APAEAPRWRDALARSATVSAPRPLAVVLPGTMGSHLSVAGDHVWLNYGALLLGGLKKL
                     RRDANGVTPTELIDQFYGPLLEFLARSHRVEYVAYDWRLSIREAAGRLADTLERCLPQ
                     AERDNQPVHLVAHSMGGLVVRCMIADGARGASLWQRITRLPGSRFLMLGTPNFGSYEA
                     MRWLTGFNPTEAKLSLLDFTQSTDDIVNLVRRYPGLLELLPFGTQDPDFSTVAPWQSI
                     KDKTLAAWDVPQSDDLRIAAATWQCLRNAAPDPRFMRYVAGSQAATVTDYQLLADDDL
                     TNPRQHLDFIATRRGDGTVTWASGTLPGVPMWFVDDTPHDALCSATQAFPAYLEILAS
                     GTTNRLPSVADAVSRGDAPDERFPLPATPPEDGIPDESGLRGFGFGGGLPAETPNAAP
                     AAPVIEVSIRHGDLSYARFPVLVGHYQGDPLVHAEATLDRQLDGALSRRVNLDIYPGR
                     LDSHAVFFRENPRLKPTGALVIGLGQVGALTPGLLEQIATPALLDYALQAARWPDERF
                     GPAGAPRSAAVSCLLVGSGAGGFPVRDSIEALLRAAVTVNRRLVDARLEGRITLDKLE
                     FLELFEDVAIGAAEALNQILADGEFAAAVSWPQRCIEPGQGGRRRIRFGDAPGWWHRL
                     DIARDGERLRFTFATDRARAEETLATGQLALADAFIRQASRSPRANPEAARTLFELLL
                     PLRLREMAPQQGDMVLLVDEDSARFPWELLEDRWSHSGRPPAVQAGLLRQLKTATFRA
                     RPSLGSEARALVIGNPDLSGSTVFSDLPGARDEATRVAGLLGTRGFETEDCIDAKATS
                     IVETLHKRPWRILHLAGHGVHRFMIAGDTPRPCSTCGQATPAPQKPISGMVIGDGIFL
                     TPGDVEQLRWVPELVFINCCHLGNTTAPADDLDRAGLAANLGVEFIRMGVRAVIAAGW
                     AVDDRAASTFAETFYGRMLAGESFGDAVRGAREQCWISHPGVNTWGAYQCYGDPAYRL
                     RPAVATTETGHTPFFAPSELVAELSNLIGQLKAHVDPDSPRIIEAILQRIPAAQGASW
                     RARADVSAALGLAWSEARCWPEAIAALEQAMQANSADLPVRAIEQWANLSARRAVECL
                     HGRAPITAAQRKEMVATLRTVIDRLQSLRTFGATIERLSLLGSTWKRLALVQTGSARV
                     AALGHMADAYGEAFALGKETDPYPFANAWTAELLQPDAPSRPERDTTCSRLLALQADA
                     NARNPSFWGDIAVADLKLARLLARCAGGGPAPRLPETCLELSQEVTEAYQAALARGAS
                     ERERASVTEHIDFLIRLTAPKSPQLAFLQTLRNTLA"
     gene            9529..12261
                     /locus_tag="PLH85_01245"
     CDS             9529..12261
                     /locus_tag="PLH85_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB63209.1"
                     /translation="MAETKSTCCYCGVGCGVIIEHDAGRITGVRGDPDHPANFGRLCI
                     KGSTLHLTAAPETLATRALHPELRTGRALPRQRVSWDTALDTAAERFAKIIAEHGPDA
                     VAVYGAGQLLTEDYYVFNKLVKGLIGTNNLDTNSRLCMSSAVAGYKLSLGADAPPCSY
                     EDIAHTSTLFITGSNTAFAHPIVFRRVEDARKANPDMKLVVIDPRRTDTAEGADLHLA
                     LLPGTDIALYNAMLHVMLWEGWVNRDYIDAHTEGFDALKRTVREYTPAMAATICGLDK
                     KVIEQAAELFATSPATLSMYCQGLNQFSFGTHNNSALINLHLATGQIGRPGAGPFSLT
                     GQPNAMGGREVGGLANLLSAHRDMANPQHRAEVAALWGVDSVPEKPGKSAVELFRALK
                     SGEVKAVWIACTNPAQSLPDQTLVHEALQSAEFVIVQEAFGHTETCAYADLLLPASTW
                     GEKDGTVTNSDRTISRVRPAVSAPGEARHDWAIVVDFARRLGTRLGKGDLAARMFPYD
                     RPEDVWNEHRESTRGRDLDITGLSYTTLEDVGPQQWPFIEGAQAGKVRLYADGVFPTP
                     SGKARFVATEYRVTAEAPSARFPLHLNTGRLRDQWHGMSRTGLVARLYSHESEPLLHM
                     HADDLARRQLSSGDLVRVKSRRGAIVVKVLESDTVRPGQCFLPMHWGANAMGGQGVNV
                     LMPPDFDPTSKQPELKHAAVQVEKFTEGKALVALRRSEPDANGVLQPNPLEVMESLRR
                     WLPHFPYASLTLAGRHEPVVVLKARGEELAPELLEALDRDLSLDDPTRVLSYQDRRKD
                     VAKRALVEGDSLTAVRLAGETRAADWLADMMVRGHSAAAVRPWLLAPVDQPPAGQSAR
                     GKVICNCFDVSEEDIVAAFKNGDTLDALQARTRCGTNCGSCLPELKRLQQQNSH"
     gene            complement(12331..13212)
                     /locus_tag="PLH85_01250"
     CDS             complement(12331..13212)
                     /locus_tag="PLH85_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HNB63210.1"
                     /translation="MNWQDRALVFECGGESLVGILSAPQGPARVGVLVVVGGPQYRVG
                     SHRQFVSLARYLAARGVPCLRFDYRGMGDASGAARHFLDVSEDIRAAMDAFLAAQPGL
                     EKVVLWGLCDGASAVCVYPASDARVAGVVILNPWVQTSAGQAKVFLKHYYLQRLSDPS
                     FWKKLLGGGVNVFGSIRSLFSTARAARGDGAARTANSSDEADAGSLPERMAQGMLRRA
                     GTPAALFLSGRDYVAREFEQVRDGSERWQAVVTRPEVSTRHFEQADHTFSGPDDCPAV
                     SAATFEWLVAQGWAPAS"
     gene            complement(13209..14036)
                     /locus_tag="PLH85_01255"
     CDS             complement(13209..14036)
                     /locus_tag="PLH85_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HNB63211.1"
                     /translation="MQPLRREAFFLPGAAHGGRFCLLTRAPEPVGSLVFVHAFAEEMN
                     KSRRMVALAARAFAERGWNVLQMDLSGCGDSGGELADVSWGTWIDDLRDAVRWATTDL
                     RVPVALWGMRLGALVVSDWIKADGTAPPLLLWQPVGNGKQHLTQFLRLKAAFDMLADA
                     DAKASMDAIRATLKAGGVVDVAGYRLPAALASGMEAATLSLPAAYAAPVVALEVAPAD
                     RQEVSPALRGPVDRWANAGTAARAAVVPGVAFWQTQEIEVCEPLIEASVEALKGFVR"
     gene            complement(14046..14294)
                     /locus_tag="PLH85_01260"
     CDS             complement(14046..14294)
                     /locus_tag="PLH85_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB63212.1"
                     /translation="MDTKKEVLSILDEVLSLGGRAAEFDLDTPLLGALPDLDSMAVVG
                     LINMLEERFGFVVEDDEIDGATFASVGSLVEFVDAKLA"
     gene            14466..15701
                     /locus_tag="PLH85_01265"
     CDS             14466..15701
                     /locus_tag="PLH85_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB63213.1"
                     /translation="MKRVLLIAFHFPPVQGSSGVQRTLRFARYLPDFGWEPIVLTAHP
                     RAYAETSDDQLKDIPPGVRVIRAPAWDSSRHLSIAGRYPGFLARPDRWMSWWLGAVPA
                     GLKIIREFNPLAIWSTYPIATAHRIGGSLARLSALPWIADFRDPMVDGDYPSDPAIRR
                     SFEAIQGNAIRRAACSTFTTPSALAMFRRQFPERANHMALLENGYDEETFAGQTATGR
                     LHPERLTLLHSGVIYPSERDPTQLFAALRLLKERAPAVLARLGVRFRAPIHFDLLADL
                     ARTYGVEEHVELAQPVPYREALAEMLNADGLLILQAANCNAQVPAKLYEYLRARRPVL
                     TLTDPAGDTAQVVRDAGIGALARLDDAADIASLLERFVAQPDAGTLPTEAAIRGASRR
                     GRTEALAGLLDRAVAHGGA"
     gene            15705..16397
                     /locus_tag="PLH85_01270"
     CDS             15705..16397
                     /locus_tag="PLH85_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB63214.1"
                     /translation="MALGKLRATFAEMGALNGGLYLLHRILQTLSGGRAQLVRYYLVA
                     QPIHPDAACRPSAKNPVTLLDPSDPLIAAFPRPPEVIAKRLRDGATCFIARSGETFAG
                     FLWLAHGAYDEDEVRCRYELVDDDRCVWDYDVYVEPAYRIGRTFARLWDAANQHLAPR
                     GVRWSISRISAFNAASLAAHDRLGIKILHSATFICLGSVQISALGGAPYLHIGMSDAS
                     RPVLRLRAPDAE"
     gene            16490..18094
                     /locus_tag="PLH85_01275"
     CDS             16490..18094
                     /locus_tag="PLH85_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HNB63215.1"
                     /translation="MNATTLLHQLVTESALRAPDAGALRDGAAELSYEALAGQIGAFA
                     SGMMALGLDRTERVGVYLDKRFETVIASFGAAAAGGVFVPVNPILKPQQVGYILQDCN
                     VRVLVTSVERFKPLADTLSDCHDLRHVILTGKVDTLPELPGMSIHRWADVMAAPARAG
                     HRVIDNDMLAILYTSGSTGRPKGVVLSHRNMVAGAKSVATYLENRADDTLLAALPLSF
                     DAGFSQLTTAFHAGARVVLLNYLMPRDVLNALVKHKVTGLTAVPPLWIQLSQLEWPET
                     VTDHLRYIANTGGRMPLETLTALRARLPRTKPYLMYGLTEAFRATYLPPDQVDVRPDS
                     IGKAIPNSDVFVLREDGSPCEPGEPGELVQRGALVAMGYWNDPEKTAERFKPLPVTAP
                     GRQAGLVLPEIAVFSGDNVRRDEEGYLYFIGRRDEMMKTSGYRVSPTEVEEILYGTHL
                     VGECVAFGVPHPSLGQAICVIATPRAGSALDSAALLAECKQRMPAYMVPSVIEVRDGP
                     LPRNPNGKIDRKTLSTEFENARKVQS"
     gene            18091..19335
                     /locus_tag="PLH85_01280"
     CDS             18091..19335
                     /locus_tag="PLH85_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HNB63216.1"
                     /translation="MSDTPREKPVHAPMDQFPVVNGELTVGGISLTRLADRVGQTPFY
                     AYDRALLTRRVQDLRATLPTAIKLHYAMKANPMPAVVDHMARLVDGLDVASGRELRVA
                     LDSGANPHEISFAGPGKSHQELRQAVAAGILINIESFREVAELAAIRQATGWHVRVAV
                     RVNPDFELKSSGMKMGGGPKQFGVDAEQVPELLAEIGRSGLAFEGFHLFAGSQNLRAE
                     SIVEAQRKCFDLALRLAESAPSPVRFLNLGGGFGIPYFPGEQRLDLAPIAANLAIIAE
                     EAAVKLPGAELVIELGRYFVGEAGLYVCKVVDRKVSRGHTYLVTDGGLHHHLSASGNF
                     GQVIRKNYPAAVGNRMDAPLGEAVSVVGPLCTPLDLLADRMALPEARPGDLIALYQSG
                     AYGATASPRGFLSHPDLVEVLV"
     gene            19578..20489
                     /locus_tag="PLH85_01285"
     CDS             19578..20489
                     /locus_tag="PLH85_01285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNB63217.1"
                     /translation="MDATDASLPAPLITTIIPTMASKARAASLKRAITSIRAASKQPV
                     AIIVVVNGTRSDPEACAWLADQPDVRLEFDAKPSAANAMLRGRELVATPFFTSLDDDD
                     EFTPGALDLRLAAMQSDTCPDIVVCNGLRRDSTGDTLCYDDLPSVPAAPLPALFRMPW
                     LSSCNSLYRTASIDAHFFRNFHSFAEWTWLAYQFGVAGKKIAVIDTPTFVCNDTAGSL
                     SKSNEYEMAYLSLYERMLSLAPPAPVADLIRRRIGSFWHDQSVTALRNGQRLDALRHH
                     VRSLRQPGGLRYLGYSRYLIPGWPQNR"
     gene            20486..21970
                     /locus_tag="PLH85_01290"
     CDS             20486..21970
                     /locus_tag="PLH85_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNB63218.1"
                     /translation="MTDSSSNSVFKGALMTVLMRWTDRLIGFISTLILARVLVPEDFG
                     VIAMASLVVGLLDVLLDLGVNVALIQNRTPSQAHYDSAWTLRLAQAATTALLIVIASP
                     FAGDYFNDPRVTPVLQFMALSLFVAGLENIGTVTFQKDMRFDLEYRFNFSKRIVAFLV
                     TVTSAWLLHSYWALVFGTLSGRIFGVFLSYRMVAMRPRLSTEKMREIFAVSQWMLVNS
                     IGIYLNANLHKMFVGRFASTGTMGGYTLADEISAMPSTEILAPLNRVLFPAFVRAKHD
                     LVELKRVFLLAQGVQCLIAVPASLGLAFIAHDAVLVLLGERWSLAIPFVQVLALSNVV
                     QAITNSGAYMLLTLGKVRSVTLTTWAQVILVAIGALLVLHQPEALDIAILRVSAVFAG
                     LVLAMWILMLNMSGLRLLELVRTIVRPILATAVMGGAIHGLGVLTADLPVVVAMFLKI
                     FAGMFSYTAAVVLIWRLAGRPDGAESYVFDKLAGVLRRRKAGTA"
     gene            22016..23830
                     /locus_tag="PLH85_01295"
     CDS             22016..23830
                     /locus_tag="PLH85_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNB63219.1"
                     /translation="MDNRFSIQGRLSPNPAGTPGELSIERLDDGWRCHWAGARLEVHA
                     PARLAERDGILCVASGSPRLPVSGADIADAWLELHARHGADVHRDVKGLFSVAFFDTR
                     RQTASFYTDRFATESWCFGEEDGALCFSDRADAVPLTRRSVSAQSIFDYLFFHVIPSP
                     VTLFREVRRLPPASRLDWQNGSTRQAPYWVPVFREDTRVSVEDAKARFMEIVRDSVTR
                     EIDRDDVGAFLSGGTDSSTVSGMLGRITGRPARTYSMGFDATGYDEMEYARIAAKHFK
                     TDHHEYYVTPEDLLEGIPKVARSYDQPFGNSSAVPAWICASRARADGIGKLLAGDGGD
                     ELFGGNARYAKQRVFGWYDTIPSPLRSGLIEPLSGLPGMDRIPLIKKGVSYVEQASVP
                     MPDRMQMYNLLLRLGLDTVFQPNFLKGIDTGEPSRLQRDTWALVPEGSLLNRMLAFDW
                     KYTLADNDLPKVIGTTRLAGVDVGFPLLSDELLDFSLTLPSEWKLKGFKLRWLFKEAL
                     RGFLPDEIITKKKHGFGLPFGVWAVKHPGLKALACDTLEAFGQRGVMSPTFLHDLHAK
                     WLPEHPGYYGELVWIVMMLEQWLQAYAPDWRLGGPARA"
     gene            23827..26055
                     /locus_tag="PLH85_01300"
     CDS             23827..26055
                     /locus_tag="PLH85_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNB63220.1"
                     /translation="MTAARPDAPSAASASRPPARAGAALLRLGLPLLLIALVWLAHYA
                     HFHVDRSPVAEAVVEQARQAPDDARLDEIGHMSLAIDLGVPSDRVVATADLIVRGTAL
                     FPAYLDGPTPLTGYPADFRFGPGTFQLAAAGFGFEDILLEAYERTADARYLRTAYQRI
                     QDFAAFELTQREAVDFLWNDHAIACRVAVVARLWRHIRHDPAYPADGLRNLLSLIARG
                     GRFLAAPDHFTARTNHGVIQNVALMQIAAAFPSLPDSAGWARLGMERLALQLPFYVSP
                     EGVVLEHSADYQHVGVSLLAMAVRLARLNDLPVSRELLDKSAASRTVQSQLQRPDGSL
                     PAVGDSIVGRPHTAVTGATDGSTPVAEQPAPFPLPEPAAHVYPVSGYALWWTGDDPAL
                     RSQTVIAWAKHDGHGHKHADEGSLLLWSGGRDWLTSIAYWPYGGANEKAAYGWSGANA
                     PHGIGELAASVRTVELLGSADDAQMHAVDLLRRTGDGAQFRRQIVQIDAQTWLVLDFV
                     DGSASGSETDWTLPPALGLTARADDRSFQSTEAEGRRLILSLAAPSTTRSRILRGSQD
                     PFAGWVSVNLRPTPASTLRVTDPSARSVTALLLALRPADRIGPQLSVAPGATPEAWSI
                     HIDEEGSRHAVTRQGARLAMDDASVLSLAAPADIAPAQAVLRAAYRKAVDAFPPWRDL
                     SRFRARIAYGLLALMVAQEIAWQVFIRRPATTPGQRRGVLLGLSAGWIALGAWIFGIY
                     LA"
     gene            complement(26241..27764)
                     /locus_tag="PLH85_01305"
     CDS             complement(26241..27764)
                     /locus_tag="PLH85_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63221.1"
                     /translation="MIRLLGRMLAVGASVLTLGAWAASPENSLPDAIDGASCHYFNGV
                     GGGRWNREGGDWADADRRSYGTRAYSTTVIREGSRGHLVEIDMTGLARDWLAGKADPA
                     QGAMLMSTSGPGGNIVVFASREHPDITARPLLRLEFKDGRRTAFDPVSDAYLDCSTEK
                     GLGFEPRLRAGGTYRSLLAFALPVEKFAGRVLDKAVLTMRVDDQYGNDTTLGVFAVRA
                     PESPDRLPRPGLAAAYVGDAGIAKDPRVLFFSDFEADRWSSAWSEYDSRSTSTTVTSD
                     PERLFLPLSGRALRVTVPRNGNLGLDLRYNYRDKTGSEPEEAYFRYYLRFAADWGPEQ
                     SAGKMPGFAGTYGKAGWGLRKSTGDDGWSLRGQFSMVSRSGNPLSGRTGMGTYAYHAA
                     MRDPSGDFWPWPLGRLAALANNRWYCVEQYLKLNTPGKSDGILRAWIDGRLVVERTDI
                     QLRNVPQLRIESVWFNVYYGGVDPSPRDMSLYIDNVVVATSYIGPMGRSSEAGAPVR"
     gene            complement(27761..28525)
                     /locus_tag="PLH85_01310"
     CDS             complement(27761..28525)
                     /locus_tag="PLH85_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63222.1"
                     /translation="MHVLSRRGRVAGWVVAGMLVASAAMAGEHGTCQPVPALEFGPYD
                     YRVAPPDKLLRVENYHFDTGVESLTKGKSSAYVGHDLEFTLRYFPNHFRALNAMVKLG
                     LKQKTPTPTGTTETVDCWVSRAIQFRPDDGLMYLINGLWLVQRGMKAEAAEQMAKARE
                     LQVDSANYQYNLGLGLLMVDKPQQALEAAHKAYALGHPLPGLRNKLQRIGAWRPQPEP
                     APEDPPGTAAPPANTGANASGAAAAAPGAAATRAGE"
     gene            complement(28580..29578)
                     /locus_tag="PLH85_01315"
     CDS             complement(28580..29578)
                     /locus_tag="PLH85_01315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB63223.1"
                     /translation="MGLLPRSLALKAEGLASLLPGKGRRLSVLIFHRVVPEPDPMNPD
                     EIDADRFDRLLTMLGREFHVLPMAEALSRRAAGTLPARSVAITFDDGYADNFTVACPI
                     LRRHGMEATFFVASGFLDGGLMWNDAVIETLRQYGGATLDLAWLDMGMCATQSVVQRQ
                     VLAEALLLKLKYLDLDGRKAALARLVAQTGSRLPEDLMMSSAQLRGLRDAGMEIGGHT
                     VSHPILAVLDEDEARSQMARDRARLAELLGRPPRFFAYPNGRPGKDYTAVHARLAREL
                     GYEAAFTTSHGSVGSESGLYELPRFTPWDHNLRKFSLRLLRNYLNVDAPQLASARA"
     gene            complement(29600..30589)
                     /locus_tag="PLH85_01320"
     CDS             complement(29600..30589)
                     /locus_tag="PLH85_01320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB63224.1"
                     /translation="MRGAFYRLARLLGLFTLASWLYRGRQSILCYHAFAFEDEHLFRP
                     QLFMQPATFARRLDLLARRGYRVITLDEALRRLDAGSARLRDVVITIDDGFISVLRHA
                     APALAARRIPAMLYLTTHYQTAARPVADLVAQYLIWRALRRHPERADALAALLPPGEG
                     GPIARYQRLDKPARRAFLRNARAAGVLEPESAGLDERFRIVTAEQVRALQAAGLSIEL
                     HTHRHRYPADPAQLRREIDDNRAALAACGVAAARHLCYPSGEWRAASFPVLEAAGVVS
                     ATTCIAGLNERTTPRLALHRFLDADDISDIEFEAEISGFKELLRGARRRLRGA"
     gene            complement(30591..31886)
                     /locus_tag="PLH85_01325"
     CDS             complement(30591..31886)
                     /locus_tag="PLH85_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HNB63225.1"
                     /translation="MRDVALTLIMLVLVPSIFFRPSLGPLAWAWVSMMVPHRLTFGFA
                     YSLPFAQLVGGATLIALFAAKERHRIPWITPIRYLAYFYAVMCFTTIIALNNVGDAFD
                     MWVKVTKVHVMLWVTIALIGGKKQILDLIKVITLSIGFYGIKGGLFTIRSGGGERVWG
                     PTGSFFEGNNELALALVTIMPLMYFMRQTAERAWWRTVLSISLILVAFSVLGSQSRGA
                     LLAISAMALILGARSKRPVLAVSLFAIIGIGLAAFMPDTWSNRMNTISSHEDHSAQSR
                     LYTWQMILNMVAHNPWGAGFDFWTWEVWGRYAVTEWLLPYSPHSIYFQALGEHGILGF
                     FFYIGMGVTSWRFCGRMIKEVGNDPEQQWLKLLAQCIQISLLSFAVGGTFLGLVNFDV
                     PYYLVAIVGLLWRDCRKVPDAVTAPGAPAVPRPRPAGGL"
     gene            complement(31958..33307)
                     /locus_tag="PLH85_01330"
     CDS             complement(31958..33307)
                     /locus_tag="PLH85_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB63226.1"
                     /translation="MGLYTRVCSGVLFPLHERLKKHDSVARRKDLEITQRLPRAEIEA
                     IQARRLKAFLEDIGRHVPYYHTLFREHGFDPAGVTGAADLRKLPFMTKAVIRAHVDEL
                     KADDAGPLSRYNTGGSSGEPLIFYIGKARKSHDVAAKWRATRWWDVDIGDPEIVLWGS
                     PIELGAQDRVKAVRDSLLRTWLLPAFEMSDAKVRDMLAMIRAKRPRMLFGYPSALAHL
                     AERARLWKIPMDDLGIRVAFVTSEMLYAHQKQSITEIFGCPVANGYGARDAGFIAHQC
                     PHGHLHLSAEDIVVETIDPEGRPVAPGAPGEIVVTHTATRDFPFVRYRTGDIGVLSTE
                     TCGCGVNLPILAEVQGRTTDFVVGADGTVMHALALIYTVRDLPGVQQFKIVQHDIGRT
                     EVLVVADDRFGEAERQRIRHDYAARLGAGVQVDITPVSEIPPEKSGKTRYVVSHVKR"
     gene            complement(33320..34414)
                     /locus_tag="PLH85_01335"
     CDS             complement(33320..34414)
                     /locus_tag="PLH85_01335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB63227.1"
                     /translation="MEQPTYSGLRLAVVGPLPPPSGGMANQCQQLLGLLRAEGAEVEL
                     VRTNAPYQPEWAGRIPVLRAFFRLLPYLWALWRASGRCQVMHVLANSGWAWHLFAAPA
                     LWIARLRGAPVIVNYRGGEAEPFFAAAPRHVHRTLAAADALVVPSAFLDGVFRRFGFA
                     SEIVPNIINLERFGVPRTRTADDAPHVVVTRNLEPIYDIPSVLAAFAAVRAALPAARL
                     TVAGSGPELTRLQAMAADLGIAEAVTFAGRIDNAQIPALYAAADVMLNPSTVDNMPIS
                     ILEAFASGVPVVSTDVGGVPFIAEDGRTALLVPARDPARMAEAMLRILRDRPLAERLA
                     AAARDEVKQYAWPVVRQKWLAVYRRLARNR"
     gene            complement(34414..35433)
                     /locus_tag="PLH85_01340"
     CDS             complement(34414..35433)
                     /locus_tag="PLH85_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNB63228.1"
                     /translation="MTALRRRVAGRAMPRLLLATVVCMAILAAFLGVQAIPSTEAVRL
                     RNALLVEPSSPADFTWTPAQVPAGFLQDAGPVEPLFADVVRNLGIAALPDDRAKALAL
                     SAHLSQHADQEGPIQSDLQTTYHAIRKGAGYCADYTAVYLALARAAGLFAREWGFSFD
                     GFGGHGHAVVEVFDRQHQRWMFLDVFNNFHPVYADSGEILSVAEFRAFLHGEGPAVRI
                     VKTGPGRAGMKYEDKLLAYYRHGMDQWYLWWGNAVFDYERAPVVAALGHVSRSLEQLG
                     AIVAGVHPHVRIVETPSNQALVARMTRLATQIRVIAAVEAVLAFLMLVQILAWRRSGR
                     RENPL"
     gene            complement(35430..36746)
                     /locus_tag="PLH85_01345"
     CDS             complement(35430..36746)
                     /locus_tag="PLH85_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB63229.1"
                     /translation="MKISIFGLGYVGAVSLACLARDGHDVIGVDIDATKLDLIRQGKT
                     PVVEEGMVDLMKDVVAAGRVTVTTDAVHAVLNSDISLVCVGTPSAANGSQDQGAVLRL
                     AESMGVAIREKAAPHVVVFRSTLVPGTVEDVLRPIIENGSGKKDGEGFHLCFQPEFLR
                     EGSSIRDYDKPPFTVVGANHDYPAERLKALFGHLPCNFLKTSVRSAEMMKYCCNNFHA
                     LKITFANETARLCAALGVDPFEVMDLVCQDTQLNISRAYLKPGYAFGGSCLPKDLRAT
                     TYLAKRHDVEIPMLAGIMQSNRNHVDVAVNKVLETGKRKVGFVGLSFKTGTDDLRESP
                     LVSLAEQLLGKGVQLSIYDPEVHLSRLLGANKSFIERHVPHIGELLKPELADVVAEAE
                     VLVVGLSSKEVFADLARLARPGQKIIDLVNLPNVADIQADVEGLCW"
     gene            complement(36743..37957)
                     /locus_tag="PLH85_01350"
     CDS             complement(36743..37957)
                     /locus_tag="PLH85_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNB63230.1"
                     /translation="MNILYVCHRFPFPPKRGGKIRPFNMIRHLTAQGHAVTVCSLARS
                     PQEAEEGRGIAPHCKAFEMGLVSNPVQALRMVARLPVSTPSSMGFFYSPDLKAKIDRL
                     LASQKWDLIFVHCSSVAQYVEHVTDVPKIIDYGDMDSQKWLEYAHYKPFPLSLGYRLE
                     GLKMLAAEKRLAGKFDLGTATTRAEWETLDSYGTGLATDWFPNGVDAGFFCPDDTVYD
                     PDTISFIGRMDYYPNQECMTRFCQDIWPRVKAARPQMKLLIVGADPSPAMRKLGELDG
                     VTVTGSVPDVRPYVRSSALMVAPLAIARGTQNKILEAMAMGVPVVTSSIAAGGVDAEA
                     EKHFLVADTPEAYAAAIARITSDPVERRRLAEAGRARMLSHHAWPASMARLDRIIGRC
                     ITQFQESKEKRT"
     gene            complement(37954..39879)
                     /gene="asnB"
                     /locus_tag="PLH85_01355"
     CDS             complement(37954..39879)
                     /gene="asnB"
                     /locus_tag="PLH85_01355"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB63231.1"
                     /translation="MCGIHGFFRFDGQRVEPAQLTAMGDRTQHRGPDDEGQYIDGPCG
                     IAMRRLSIIDLAGGHQPISNADDSLWIVCNGEIYNFRELRAELQGLGFHFKTGSDSEV
                     VLHAYDAFGDDFVHRLNGMFDFALWDRRRKRLLIGRDRIGVKPLYVMQDARRLAFATE
                     AKALLALPGVSPELDRSQLASYLHLGYAAAPGSMFKGIRKLPPATLLSIENNEVREWR
                     YWRLPSVVERTVSEADWLERVRVRLEESVRMQMVSDVPIGAFLSGGVDSSAVVGYMAR
                     HSDRPIRTYAIGFEGGEAEALYNELPYARQVSQLFGTEHHEIVVKPDVVSLLPKLLWH
                     MDEPLADTAFITTFLVSEFARQDVKVILSGVGGDELFGGYRRYLGGHYAERFQRLPGF
                     VRSAATAVANRLPSDRHSGLLNTLRLAKGFIASANMAPDDRYRSYLQVLARETVSAML
                     LDAGAQAPDPLAQAFSDAGQADDLNRMFAVDAETQLPDDLLLLTDKMSMAVSLECRVP
                     LLDHELVELAAAIPAAVKIRGGRLKHLMKDALADLLPADILDRKKRGFGTPMGAWLKR
                     DLAPVLRQLLSPESVRRRGLFRHDVIAGLMADHESNRSDGTDGLLSLMNLEIWSRIYL
                     DGRSADDVAAELKSYTR"
     gene            complement(39889..41094)
                     /locus_tag="PLH85_01360"
     CDS             complement(39889..41094)
                     /locus_tag="PLH85_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HNB63232.1"
                     /translation="MRVLHVLDHSLPLHSGYAFRTVSILREQRKLGWETLHVTTPKQG
                     VSSGGAEEADGWTFHRTANTGQEGLVAQMRLTAARLDDLVQTLRPDILHPHSPVLNAL
                     PALWVGRKHRIPVVYEMRASWEDAAVDHGTTAEGSLRYRVSRGLESFALRHADQVTTI
                     CEGLRGDIAVRGVPDERITVIPNAVDVSAFTFGAEADPELRRSLGLDGKTVLGFAGSF
                     YGYEGLDLLIRAARQLVDRYPDLRVLLVGGGPQDANLKALVAELGLSDRVIFSGRVPH
                     AVVQRYYELIDVLAYPRLPIRLTELVTPLKPLEAMAQGRMFVASDVGGHKELIRHGET
                     GFLFRAGDVDALAAAIDDVLTHRERWPQVRQQARSFVEVERTWATSVARYRDVYRRAL
                     AARGRTLSI"
     gene            complement(41097..43022)
                     /locus_tag="PLH85_01365"
     CDS             complement(41097..43022)
                     /locus_tag="PLH85_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HNB63233.1"
                     /translation="MCGIAGIFDTRGQQDYPRELMQRMNDIQSHRGPDESGYHFEPGV
                     AFAHRRLSIIDLSTGQQPLFNEDGSVVVVFNGEIYNYQELIPELTALGHRFHTRSDTE
                     VIVHAWESWGEACVQRFRGMFAFALWDRNRQTFFMARDRMGVKPMYYAILRDGTLVFG
                     SELKVLMQHPDLDRTMDPLAVEEYFALGYVAEPRTIFKGARKLDPGFTLTIRRGEPIP
                     QPKPYWDVRFTLDHNLNLADATAELKERLKESVRLRMIAEVPLGAFLSGGVDSSAVVA
                     TMAGLSDEPVNTCSIAFDDPAFNESEFAQMVADRYKTNHRVETVQSDDFDLIDTLAAL
                     YDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDENMGGYRRYRFHMMEEKMRGGLPY
                     GLRRPLFGFLGSVYPKADWAPRVFRAKTTFQALARDSVQAYFHSVSILRDDMRRQLFS
                     QRFRQELGGYNALEVFRRHAANAQTDDPLALIQYLDIKTYLVGDINTKVDRASMAHSL
                     EVREPLMDHPLVEWLASLPSSLKIKGQEGKYVFKKSMEPMLPRDVLYRPKMGFAVPLS
                     RWFRGPLKERVREALLGETLRATGLFNEAYLRHLVDAHQSGARDYSSAIWTTLMFEAF
                     LRNCQTTAQPAPLVRSA"
     gene            complement(43065..44231)
                     /locus_tag="PLH85_01370"
     CDS             complement(43065..44231)
                     /locus_tag="PLH85_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNB63234.1"
                     /translation="MSALHPDGRLLVMHVVYSFAVGGLENGVVNLINRMDRTRFRHIV
                     VALTQCEPLFCARVQVPDVEFVALHKPPGQGVKIYPALFRLFRAYRPDVVHTRNLAAL
                     EATVPAWAAGVPVRIHGEHGWDVSDPHGTRAKFRWMRRLYRPFVTHYIALSGQILSYL
                     VRAVGIPDRRAERICNGVDVARFTPRGARRAALEGSPFNDPAYFVIGTVGRLQAVKDQ
                     LNLVRAFARMRQLDATHSESVRLVLAGDGPLRGRIEDEIRTLGLNGRVWMAGERSDVP
                     RMMSGFDLFVLPSQTEGISNTILEAMAAGLPVVATDVGGNGELVADGESGALVPPQDS
                     QRLADKLLEYVRDPLRGRAEGHAGRLRVEANFSLDGMVKRYADLYLALCAAKGR"
     gene            complement(44228..45733)
                     /gene="xrtA"
                     /locus_tag="PLH85_01375"
     CDS             complement(44228..45733)
                     /gene="xrtA"
                     /locus_tag="PLH85_01375"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HNB63235.1"
                     /translation="MNSRRAVPVAIAGVFLWIIGAYFTTAADIAGVWWRSESYAHGLM
                     VIPIFAWLVWRKREVLATRPLRAWPIWLLPVAVAGFGWLLGQLGSVASVSHFCLVTML
                     VLTLFGVLGREAAGALKFPLLFLLFGIPAGEFLLPVMMKYTALFTVSAVRLMGVPVYQ
                     EGLHFVLPNGRWSVIEACSGFRYLIASVMVGTLYAYLSFRSLRRRLIFTAVAIVMPVV
                     ANWLRAFMIVMLGYFSNNQLATGVDHLIYGWVFFGLVILLMFWVGSFWREDLDELPAA
                     AAVPAPAASIDTAALVRGVLPFALLTAVWPVVAVLLAAPTDRDVTIPIALPAAGGAWR
                     AGGVETGHAFQPRFSGHRGLMEEGYSDGRRTVNAYVAVYANQSQGRELVRAGNVLLSV
                     EDHHWAMLDEGAGPETPFGAWKRSVLSGEGTRIVVWSAYWIDGRLVTSDYVAKGLLTL
                     ARLTGKADTSAYVAMWSRAPDERSAEQALEDAAASLQGPLFQQLQSLDRVK"
     gene            complement(45804..46841)
                     /locus_tag="PLH85_01380"
     CDS             complement(45804..46841)
                     /locus_tag="PLH85_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNB63236.1"
                     /translation="MASPISVRRFQAGDSTRWNAFVFDCPDATFFHRIEWRDIIERVF
                     RHRCHYLLAERDGHIVAVLPLVEVNSRLFGHSMTSLPFAVYGGVAGSDADAVAALEAE
                     AETISRQAGVQHLEYRNLRARHEDWPRQDLYVTFRKEILPDEEANMLAIPRKQRAMVR
                     KGIKNGLVSHVDPNADRFFALYSDNVLRHGTPALPKRFFETLLEVFGKDCEVMTVTTA
                     DGKLLSSVLSFYFREEILPYYAGDDMEARDLAANDFKYWELMRRACARGCRLFDYGRS
                     KIGTGPYSFKKNWGFEPMPLSYEYRLFKRDEIPQNNPMNPKYRAFIALWKRLPVGVAN
                     RLGPMIVRNLG"
     gene            complement(46852..47721)
                     /locus_tag="PLH85_01385"
     CDS             complement(46852..47721)
                     /locus_tag="PLH85_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNB63237.1"
                     /translation="MNPHVDDARSLVNALTCDVEDYFQVSALAPHFPRSGWDGTPCRV
                     EANVDRILALMDAAGAKGTFFTLGWIAERYPALVRRIAEAGHEIASHGYGHERASDQS
                     PEAFLCDIRLAKAILEDITGLEVRGYRAPSFSVGLSNPWAHDCIQAAGYVYSSSVYPV
                     RHDHYGVPDAPRFTYPVGNSLVEIPITTVRRFGRNWPVGGGGYFRLLPYGVSAWGIRQ
                     VNAGDRKPAIFYFHPWELDPGQPVVLEASAKTRFRHYVNLERTEARFTRLLNDFAWGR
                     MDEVFLSGDTPSA"
     gene            complement(47718..48794)
                     /locus_tag="PLH85_01390"
     CDS             complement(47718..48794)
                     /locus_tag="PLH85_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HNB63238.1"
                     /translation="MYESHFGLRGKPFQLNPDPSFFYGSRGHRRAMAYLEYGLHQNEG
                     FIVITGEVGAGKTLLVRSLLEKLDPSKIVAANLVSTQIDADDILRLVAAAFGIPSRQM
                     SKSDLLVAIETYLVSVAAAGKRALLVVDEAQNLTPRAVEELRMLSNFQLEDHALLQSF
                     LVGQPEFRAIMQSPQMLQLRQRVIASFHLGPLDATETQAYIEHRLNCVGWRGVPSFDA
                     DALAAIHASSQGIPRRINAVCDRVLLAAFLTEQRRISAAIVEQVVAEMREEQVAPPAM
                     QADTPAAALSAGLAGVDLARLAAEPEALRTLAKAGEGFDTARFEERLALLERTLSTTL
                     NALGQLLQAVQKPSQTAGKETESR"
     gene            complement(48801..50330)
                     /locus_tag="PLH85_01395"
     CDS             complement(48801..50330)
                     /locus_tag="PLH85_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HNB63239.1"
                     /translation="MSVLTIPLALTSMSVLAQDHQVTFSPSLRLTETITDNVDLSRGS
                     GTGGNASSKSDYITGIIPSMELNSRGGRAQGNLRASVASLFYANNSNLNTSFLTFNGT
                     GSVEAVEKFAYVDVRGSESREVASVLGVRPGDQVTGRANQTNVRNVGISPYLRGHFHN
                     SGVAELRYDINSSSASSGSFQRMQQQMWSGSLVDETAFGQSGWSLAFSDNQVSQTGRR
                     DLHLQSARLTLMSRVTPQLQLRVIGGGEGNNYNSSNTQQSTIVGAGFDWKPSAFGRVS
                     ATWEDRFFGPGYQMSGDYSTGPFVFNAKFSKDVTSTSQALSATSLTTAYDLLMGLLAS
                     RYPDPVARDQAVRTYLTNQGVSTDQQIGISQSVLNNGVFLDRRLQFGASYVGLRRTTF
                     MFSGYRSERSSLVSQTAALGGEFINATRVTDSSGSLMLNHRLTPLTSATADMTLIRTQ
                     ADGSATTPSFTNKTRRATAGLSTSFTRRSSGSLTYRHSRATGTTEYTENALLGSVIIL
                     F"
     gene            complement(50381..51433)
                     /locus_tag="PLH85_01400"
     CDS             complement(50381..51433)
                     /locus_tag="PLH85_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HNB63240.1"
                     /translation="MSIIEKAVERLEQLQKAAAAASAGATVEAAPVAGSAEGRIEPVG
                     TPVEQPAPVIAAVTAAVTVEAAPVAEVPLPVPSAAPEASVSSRPRASTARSVEIDLER
                     LASIGVVTPNAPRSLIGDEYRIIKRPLLRNIQGKGAAAVDSANLIMVTSSLPGEGKSF
                     SSINLAISMAMELDHTVLLIDADVSRPSILNILGLPPKKGLMDVLTTDDVELGDVLLK
                     TNIDKLTLLPAGTPHPRATELLASDAMNNLLAELAVRYSDRIVVFDSPPLLVTTESRV
                     LATHMGQIVMVVESNKTTQAAVKQAISTIEACPVKLMLLNKALQAGSGAYGYGYGYGY
                     GYGYGYGYGQSQKENA"
     gene            complement(51443..52999)
                     /locus_tag="PLH85_01405"
     CDS             complement(51443..52999)
                     /locus_tag="PLH85_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNB63241.1"
                     /translation="MEDILRQLSVILRGMWLYRWWGLAAAWIVGPIAAATVYLLPDRY
                     ESSARVYVDTQSILRPLMSGLAVQPNVEQQINILSRTLISRPNVEKLIRMADLDLAVK
                     TKEEREALIVGVTKALEIREAGRDNIYTLAYKDTNAERAKRVVQSLVSIFVESGLGDK
                     RKDSDTARKFIEEQIKTYEQKLEEAENRLKEFKLRNLSTMGAGGRDYFSKMGEVNEQL
                     NQARLALREAENSRDALKRQLAGEDPVLLPENPSSSALASVSVPELDGRIEAQKKNLD
                     SLLQKYTDQHPDVIGTRKVIEQLEAQKNQEIQSRRKAGPSTVGSINSNPVFQQMKMSL
                     AEAEANVASLKARVSEYEARQQNLSSASRMVPQLEAEYTQLNRDHDINKKNYDALVAR
                     RESATMAVEMGATSGVADFRLIDPPTLPSKPAAPNRLLLMPGAGLAALLLGAVVSFMM
                     SQIRPVFTDMQVLRDVTGLPVLGAVSMIADPERMGTQRRGRIVFLGGVGALVGLFGAM
                     TVWLLLAKVA"
     gene            complement(53058..53663)
                     /locus_tag="PLH85_01410"
     CDS             complement(53058..53663)
                     /locus_tag="PLH85_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNB63242.1"
                     /translation="MKRIVTTGLLTLAVVLATGCATKLPPPPATATTPEYNYLIGPGD
                     KVNIVVWRNPELSMTVPVRPDGKITTPLVEDLPASGRDSSTLAREIEKALSKYIRDPV
                     VTVIVTDFVGPYAEQVRVVGEVARPQTLSYKQKMTLLDVMIAVGGLTDFADGNRAKIL
                     RTSDNNAEYGVRLRDLIKRGDVSANAEVKPGDVVIIPQSWF"
     gene            complement(53856..55163)
                     /locus_tag="PLH85_01415"
     CDS             complement(53856..55163)
                     /locus_tag="PLH85_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63243.1"
                     /translation="MKTTTLARNAAALLAALALCTGTPVHADEEGDDAGLDVADEGAR
                     TTGVLQFDLARTYASPEHWSMARSRLELGATGGSAGLKWKISGRADADAVFAIDNGFY
                     PDAVRRDQQLGFDLRETYVDFSVGDVDVRLGRQHIVWGEMVGLFFADVVSARDLRTFY
                     LPDFDQLRIPQWAARGEYYFGDTHAELIWIPVPSYDRIGKPGAEFYPIPAGVPVLGEV
                     KPSNSLGNTNWGGRLSTLAAGWDVSGFYYRSMDVSQTFFRLGSITSPVFEPRHLRISQ
                     AGGTVSKDLGAFVLKGELVQTRGREFNTLDPTVDFGLIAQNTLDYVVGVDIPAFRDGR
                     VNLQYFARRYLDFDPRIGQDRIEGGASVLLNTKLSNNWEAEALFVSSVNRTDFMFRPK
                     LVWSASRNLKVVFGVDSFGGQSTGLFGMYDTRDRAYTQARWSF"
     gene            complement(55169..55915)
                     /locus_tag="PLH85_01420"
     CDS             complement(55169..55915)
                     /locus_tag="PLH85_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB63244.1"
                     /translation="MSIIRVENVSKTYLLGEQTVTALEGVSLVVEPGVFMAIAGPSGS
                     GKSTLLNIMGCIDTPSKGRVEIEGIDVSGQTPDSLADVRARTLGFVFQTFNLLPVLSA
                     QENVEYPLLQMTELTKEERAERVAFYLNMVGLTKYADHRPNQLSGGQRQRVAIARALV
                     THSRVVLADEPTANLDRRTGESILNLMREINQNAGTTFVFSTHDKRVMNRADRLVRME
                     DGQITALGMKREGSWIFVQDRRKPEDDPEI"
     gene            55958..56743
                     /locus_tag="PLH85_01425"
     CDS             55958..56743
                     /locus_tag="PLH85_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNB63245.1"
                     /translation="MTPQNPLWPRRNLLFRLLPAALLALSSLPIHAEDDEFAKNLVAK
                     ADEVRFPREGFQVDIIVDSTSPGEDPDQHKYRILSKGNENTIVQTLEPASDRGQAMLM
                     RGRDLWVYLPNVSQPVRLSLAQRLTGQVANGDLARANFAGDYDPKLLRTDTLDGDKVH
                     VLELRANDRSVTYSKVLYWVRASNNYPVRAEFYSASDRLMKTCRFEKYQTLGGRARPT
                     QLIMEDALRKGDMSVLHYSHMALKDLPDRIFTKQYMDKLNRVD"
     gene            complement(56758..57438)
                     /locus_tag="PLH85_01430"
     CDS             complement(56758..57438)
                     /locus_tag="PLH85_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNB63246.1"
                     /translation="MTQVDPREVRALLRSCNQGALGTRSVTMPGYPFVTLLPYALDGG
                     GAPLFLISALAEHTRNLLADSRASLLVAETSTSPLEQPRATLMGHVVPCDLEPAARER
                     YLSYRPEAADYLQLGDFRFFRMGIERVRLIGGFARMGWCDAPATGWQPTATVERELTA
                     ELARTAPAGVTILGVDADGADLRIAGVVRRLDLDEPAGGAEALRQALYAGLPRIAPPA
                     GEAPVTLA"
     gene            complement(57446..58192)
                     /gene="rlmB"
                     /locus_tag="PLH85_01435"
     CDS             complement(57446..58192)
                     /gene="rlmB"
                     /locus_tag="PLH85_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNB63247.1"
                     /translation="MSESASSRLIYGFHAVLAKLRHDPEAVFEIYIDAQRSDGRARDF
                     LRQAEQLGVRLIPSEGERLDKMVGTRRHQGVVARIDGRRREVMLPDLLDSLDGAALLL
                     VLDGIQDPHNLGACLRVADAAGAHAVIAPKDRAVGLNATAVKVASGAADTVPYITVTN
                     LARALREMQEAGVWVLGTAGEADKSLYEVEQKASVAWVLGAEGDGLRRLTRETCDELV
                     RIPMYGSVESLNVSVASGICLFETRRQRGA"
     gene            complement(58185..60761)
                     /gene="rnr"
                     /locus_tag="PLH85_01440"
     CDS             complement(58185..60761)
                     /gene="rnr"
                     /locus_tag="PLH85_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNB63248.1"
                     /translation="MPKPKTPKKLSAFRRSDPFYEREVATYEFPLPSREFIEQVLADR
                     GVPLSLADLVDAMDIASHEREFFERRLFAMERDGQLVRNRRNAYLLPNKADLIRGTVQ
                     GHPDGYGFLVPDGEGQDIFLGPNEMREVLHGDRALVRIAGTDRRGRPEGKLVEVLERA
                     NTRIVGRVLVENGVTLVVPENRRIAQEILLASAGKAKVTAGQVVVVELVQQPTRYAQP
                     VGRVVEVLGNYADPGMEIEIALRKHELPFEFPSAAKEQTRRLPDKVRKTDWKGREDIT
                     GLPLVTIDGETARDFDDAVYCERQGRGFRLVVAIADVSHYVRPGSALDDSAYERGNSV
                     YFPRRVIPMLPEKLSNGLCSLNPQVERLCMVCDMAITQTGTIKQFRFYPAVMFSHARL
                     TYTQVAAALYDQDPAALETVGALLPHLQNLDKLFRVLLKARAKRGAIDFETQETRMIF
                     DDQGKIEKIVPESRNDAHRLIEECMLAANVCASEFLQRHEHPALYRVHEGPTPEKLVK
                     LREFLGSFGLQLPGGDDPHAGDYARLLASLQGRPDLQLLQTVMLRSLRQAVYSPDNLG
                     HFGLAYEAYTHFTSPIRRYPDLLVHRAIKAVLTGERYQPPQESGEWEAIGLHCSMTER
                     RADEADRDVENWLKCYYMRDRIGEEFIGSVSAVVPFGLFVALDDVFVEGLVHISDLGN
                     DYYHFDDAHHTLVGERTGKRYRLSDRVRVQLVRVNMEAGKIDFRLLEGPLPARQPEAE
                     RGDAAAETPAPARRRRVANGTAGAEVMAPIVSPESPVQTAPRRARSASKTGDAGGSPV
                     KPAKSVQTSKAAKPSKAAKASKPSKPTKTSSKPAGSRASTSTGPSKRAAGGTKKKAKS
                     RV"
     gene            60801..60885
                     /locus_tag="PLH85_01445"
     tRNA            60801..60885
                     /locus_tag="PLH85_01445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60835..60837,aa:Leu,seq:cag)
     gene            complement(61060..62370)
                     /locus_tag="PLH85_01450"
     CDS             complement(61060..62370)
                     /locus_tag="PLH85_01450"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNB63249.1"
                     /translation="MAKNVVVVGTQWGDEGKGKIVDWLTDQAEGVVRFQGGHNAGHTL
                     VVGSNEYKLNLVPSGIVREGVKCFIGNGVVLDIHHLLSEVRGLESGGIKVRERLRISP
                     GCPIILGYHAAVDRAREARKSAGDKIGTTGKGIGPTYEDKVARRALRVYDLFNPARFA
                     EKLKELLEYHNFVLTQYLGADPVDFQSVYDQAMADAEEIKPMVCDVSADLYALNKAGG
                     NLLFEGAQGTLLDIDHGTYPFVTSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVG
                     GGPFPTELDFETPGTPGYQMSTVGREFGTVTGRKRRCGWLDLAALKRSIIINGVSGLC
                     ITKLDVLDGLTELKLCTGYMLDGKRVDLLPMGADEVVRCEPILETMPGWTGSTFGART
                     WDALPPEARAYLHRIEEICEVSIDVISTGPERDETILKRHPFGA"
     gene            complement(62375..63535)
                     /locus_tag="PLH85_01455"
     CDS             complement(62375..63535)
                     /locus_tag="PLH85_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNB63250.1"
                     /translation="MRWVLPEHIQDVLPAEAEKLESLRCRLLDAFRLNGYQLVMPPLL
                     EYLESLLTGAGQDLQLKTLKLVDQLSGRTMGVRADITPQVARIDAHLLNRQGVARLCY
                     CGSVAHALPSSLTATREPLQLGAELYGHAGLEADAEVIRLLAESLQIAGVTTSRIDLG
                     HVGLFRALAARAGLRDEQQESLFDCLQSKDIPGLRDLLGDAPEAVRSALLVLPTLYGG
                     PEVLERARGVLSGLDDADVRGALDDLESLSRLLADLPISFDLADLRGYHYHSGVMFAA
                     YGGTSPVALAQGGRYDRVGTAFGRGRPATGFSLDLRELAAQTPDGAGRGMILAGECSA
                     PGFAQEVGRLRASGEIVVIELPGHDGTWREAGCDRRLVLRGGAWQVEPLQGE"
     gene            complement(63551..63736)
                     /locus_tag="PLH85_01460"
     CDS             complement(63551..63736)
                     /locus_tag="PLH85_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNB63251.1"
                     /translation="MGSSLFLAFALMLVLEGLLPFVAPKMWRETFTRLVRMSDGQIRF
                     IGLSSMLVGLIVLIVFS"
     gene            complement(63736..64659)
                     /gene="hflC"
                     /locus_tag="PLH85_01465"
     CDS             complement(63736..64659)
                     /gene="hflC"
                     /locus_tag="PLH85_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNB63252.1"
                     /translation="MRDRAPFFIGVLLFVVALMSMSLFSVDQRQYALVFQLGEVKGQL
                     TEPGLHVKIPLIQNVRYFDRRILTMDSNEPERFITSEKKNVLVDLFVKWRIVDPKLYY
                     ISVGGDESRAKTRLEQTVNAGLREEFGKRTVHEVVSGEREKIMDEMRVKADQDARKIG
                     VQIVDVRLKRVDLPLEVSESVYRRMEAERKRVANELRSQGAADAEKIRADADKQREVI
                     VAEAYREAQKVKGEGDAKASAIYAQAFSQNPEFFAFYRSLDAYRNSFKSKSDVLVLEP
                     NADFFKYMKSAGGRGTATAPADANKGGKSAK"
     gene            complement(64659..65924)
                     /gene="hflK"
                     /locus_tag="PLH85_01470"
     CDS             complement(64659..65924)
                     /gene="hflK"
                     /locus_tag="PLH85_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNB63253.1"
                     /translation="MSINDPKWGNRGGGDNQGPPDLEELWRDFNRRLSGMFGRQPGRG
                     GDGGGDSGGGDRMPELNFRQFGGGVGLILALAVVIWLASGFYIVDASQRGIVLQFGRF
                     VEATEPGLRWRLPYPVQTHELVNQTGVRTVEIGYRGSERNKVLKEALMLTDDENIVNI
                     QFAVQYILKDPQQYLFNNRNADDSVIQAAETAVREVVGKSKMDFVLYEGREQVAANAH
                     KLMQDILDRYATGIQISKVTMQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDV
                     IPRARGTASRLLEEADAYKGRVVAQAEGDASRFRQIYTEYAKAPEVTRQRMYLETMQQ
                     IMSNSSKVVIDSKGSGNLLYLPLDKLIAATAAQASGAVTPEAAAQTRPQVPAAAVAAD
                     NSPAPPRPDALSGESRTREAMRNRERGDR"
     gene            complement(65976..67112)
                     /gene="hflX"
                     /locus_tag="PLH85_01475"
     CDS             complement(65976..67112)
                     /gene="hflX"
                     /locus_tag="PLH85_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNB63254.1"
                     /translation="MFDRPESGNRAIIVQVDFGQGGIDERLAEVSLLASSAGASVVGV
                     VQGRRQAPDPAHFVGTGKADEIGLLARMNEADLVIFNHDISAAQQRNLEKRLNCRVVD
                     RSALILDIFALRAKSHEGKLQVELAQLEHMATRLVRGWTHLERQKGGIGLRGPGEKQL
                     ETDRRLLGERVKLLKDRLRKLERQRAVRRRARERRDVLSVSLVGYTNAGKSTLFNALT
                     KAGAYAADQLFATLDTTSRRLYLGDEAGTIVLSDTVGFIRDLPHALVAAFHATLEETV
                     AADLLLHVVDSASDDRDGQIDAVNGVLHEIGAGDIPQILVWNKIDATLASPEVQVDAY
                     GKISRVLLSARTGEGLDLLRQILADVALAHKAALAAPSDQPQLG"
     gene            complement(67182..67424)
                     /gene="hfq"
                     /locus_tag="PLH85_01480"
     CDS             complement(67182..67424)
                     /gene="hfq"
                     /locus_tag="PLH85_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNB63255.1"
                     /translation="MSNKGQLLQDPFLNALRREHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVNIQQEQNEAAS"
     gene            complement(67528..68856)
                     /gene="der"
                     /locus_tag="PLH85_01485"
     CDS             complement(67528..68856)
                     /gene="der"
                     /locus_tag="PLH85_01485"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNB63256.1"
                     /translation="MKPTLVLVGRPNVGKSTLFNRLTKTRDALVADFPGLTRDRRYGL
                     GRVDEREFLVVDTAGFDPVAKDGIVHEMAKQAEQAIAEADALLFMVDARSGLTPHDEQ
                     IAIRLRRAGRPTFLVVNKAEGMNRAMVSLDFHALGLGEPYCISAAHGDSVKALMELVV
                     DTFPSDGGEDEAPTQGPRVAIVGRPNVGKSTLVNALLGEERVIAFDMPGTTRDAIEVP
                     FERNGKHYTLIDTAGLRRRGKVFEAVEKFSVIKTLQAVEQCNVAVLVLDASQEISDQD
                     AHIGGFVVEAGRALVVAVNKWDDVDDYRRERIKQDIARKLPFLGFARFLYISALKGQG
                     IPPLLRSVDGAYAAAMTKLATPKLTRLLLDAIVKQQPPRHGGFRPKMRYAHQGGMNPP
                     IIVIHGNALDHVPASYVRYLERSFQEAFKLQGTPLRIQFKSSHNPYADRD"
     gene            complement(68870..70006)
                     /gene="bamB"
                     /locus_tag="PLH85_01490"
     CDS             complement(68870..70006)
                     /gene="bamB"
                     /locus_tag="PLH85_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNB63257.1"
                     /translation="MRRLGRVAALIAAFGLLGCSSLNPFSSSGPKIPPLKPITDSVKL
                     GEDWKLRVGSAGTSVFSPLVVGSAVYAAAADGTVVRVEDGRTVWKVSLKTGLSGGVGG
                     SAEMVLVASPKGEVYALDPASGAIRWQANVNAEVLAAPALGDGVVVVRAADSRLFGLE
                     PADGKRRWVYQRATPALALRSAAGVVMADRAIFAGFPGGKLAAVNVNTGALMWEGTVS
                     VPRGATELERVADISSPPVLNGRAACGVAYQGRVACFDLANGSTLWTRDLSSARGLDL
                     DESHVFVTDDKGAVHALDLTSGASMWKQDQLAGRSPGRPLAFRDMVAVADVEGIVHVL
                     RKDDGTFVGRQRTDGSPVLADLRRVGSDLLVQTRDGSVFAFGVQ"
     gene            complement(70003..70656)
                     /locus_tag="PLH85_01495"
     CDS             complement(70003..70656)
                     /locus_tag="PLH85_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB63258.1"
                     /translation="MAAYDLEEQEQISQIKAWWDTYGNLVTGIALAAAVAAVGWQGWN
                     WYQGKQSGEASVLYAAVQKAAAENKPQQAREAAGMLIEKYSGTAYADLGALLSSRVQA
                     DAGDLKSARAQLGWAAEKANDPALRELARLRLAAVLLDEKSYDEALAQLQAKPSEALQ
                     ARYGDLRGDILAAQGKVADARGAYKAALEALASATKPEAVAFKEVVQAKLDGLGGAQ"
     gene            complement(70643..71938)
                     /gene="hisS"
                     /locus_tag="PLH85_01500"
     CDS             complement(70643..71938)
                     /gene="hisS"
                     /locus_tag="PLH85_01500"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNB63259.1"
                     /translation="MSKTLQAVRGMNDILPDDAEVWEQFEDIVRDWLRSYGYRPIRMP
                     IVEPTPLFKRAIGEVTDIVEKEMYSFVDALNGEQLTLRPEGTASCVRAAIQHSLIFGQ
                     PRRLYYHGPMFRHERPQKGRYRQFHQVGVEALGFDGPDIDAEHILMCARLWDDLGLED
                     VKLELNSLGSADERAAHRAALIAYLEGYKERLDEDGQRRLHTNPLRILDTKNPDLQDI
                     VEAAPRLMDFLGDESLRHFDGVQALLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTRL
                     GAQGTICAGGRYDGLIAQLGGKPAPACGFAMGIERLLVLWQESGFIPEPLTPDVYLVH
                     AGEAAQRAAFRAAEALRGTGFTVTLHCGGGSFKSQMKRADASGATYAVVIGDDEAAAG
                     EVSLKPLRSGGEQIRIPMEILPETLADRLLELEDDDGGL"
     gene            complement(71963..73228)
                     /gene="ispG"
                     /locus_tag="PLH85_01505"
                     /gene_synonym="gcpE"
     CDS             complement(71963..73228)
                     /gene="ispG"
                     /locus_tag="PLH85_01505"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNB63260.1"
                     /translation="MSTDTMDLLLSGRAAARHPTRQVRVGRVLIGSDAPVVVQSMTNT
                     DTADDFATAMQVAQLARAGSELVRITVNNDEAAKAVPRIRERLLQMNLDVPLVGDFHY
                     NGHTLLTHHPACAEALAKFRINPGNVGQGAKRDTQFAAIVELACQYDKPVRIGVNWGS
                     LDQSVLARIMDENARRAVPRDAGAVMREALVTSALESAAKAEEYGLAGDKIVLSAKVS
                     SVQDLIAVYRDLAARCDYPLHLGLTEAGMGSKGIVASTAAMAVLLQEGIGDTIRVSLT
                     PEPNGSRTQEVIVAQEMLQTMGLRAFTPMVTACPGCGRTTSTLFQELASDVQSFVRDN
                     MPVWREQYDGVENMTLAVMGCVVNGPGESKHASIGISLPGTGESPAAPVFEDGVKTLT
                     LRGDNIAGEFKAIVERYVARTYPKKGAAA"
     gene            complement(73225..74202)
                     /locus_tag="PLH85_01510"
     CDS             complement(73225..74202)
                     /locus_tag="PLH85_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNB63261.1"
                     /translation="MNDSVDNAVSLASSSVPGVTLRGERERQGLSIAEVAATLKLTPR
                     QIEAIEAGRFEDLPGFAFAKGFVRNYARLLHLDPQPLLAALEAPDDARLMELTPVSNA
                     KGDMPQVGRGRFRRSVLPGVVAALALLSVVVVGWYYDSLRRKPAEELMASLPQTPPAV
                     ATPVEPPVPAVPPAGESAAAGVPAPVPVDGAAAVAAPAVLPAGTAGTATPVPAVPTPA
                     VISAAPLTESRTAEAKAPPAVEARVGDGASERLDFDFEQDAWVEVKDGRGKTVLSKLG
                     QAGGRLGVEGQAPFTLVVGNARYVKLQRNGKPVDLAASTKVTVARLKLE"
     gene            complement(74199..74996)
                     /gene="pilW"
                     /locus_tag="PLH85_01515"
     CDS             complement(74199..74996)
                     /gene="pilW"
                     /locus_tag="PLH85_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNB63262.1"
                     /translation="MKRLLGVGALVALLGGCAVPAGPAPSGSYVAQRPQSQAPVTTDA
                     RRKARVHVELGQAYLEAGRYGVALDEAKMAAAYDPGYAPTFQLMGLVHMFLEENDVAA
                     ANFEHALRLVPGDPEIANSYGWFLCSMGKEKAGIEQLAGAARNPYYQTPTRAFTNMGL
                     CYLRLKDDGAAEQQFIRALEADNGNRQALFQLAAIQYRRANYEGAKRVIVDLHEAGEP
                     TPESLWLAIRIEHRLGDRTAEASLVQQLRRRFPASPEYQAFLQGQYQ"
     gene            complement(74993..76096)
                     /gene="rlmN"
                     /locus_tag="PLH85_01520"
     CDS             complement(74993..76096)
                     /gene="rlmN"
                     /locus_tag="PLH85_01520"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNB63263.1"
                     /translation="MELPVVNLLDFDAAALTDWFVQQGEKPFRAKQVLRWVHRFGVTD
                     FEQMTDVAKSLRARLAETACITPPVPVRDSVSTDGTRKWLLDVGNANAVETVFIPETN
                     RGTLCISSQAGCALDCAFCSTGKQGFNRNLTAAEIIGQLWLANRLLGATGDGERVISN
                     VVMMGMGEPLANFDNVVSALRLMLDDNAYGLSRRRVTVSTSGIVPAMDRLRDACPTAL
                     AVSLHASNDALRDRLVPINQKYPLRELMAACQRYLVKAPRDFITFEYVMLDGVNDTDA
                     HARELVTLTRDVPCKFNLIPFNPFPQSGFDRSPAPRIRRFAEILIDAGIVTTTRKTRG
                     DDVDAACGQLAGQVQDKTKRTAARVVPIAEVRP"
     gene            complement(76142..76567)
                     /gene="ndk"
                     /locus_tag="PLH85_01525"
     CDS             complement(76142..76567)
                     /gene="ndk"
                     /locus_tag="PLH85_01525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNB63264.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYARFEGAGLKIIAAKLAYLSE
                     REAGEFYAVHKERPFFKDLVSFMTSGPVMIQVLEGEGAIAKNRELMGATDPKKAAPGT
                     IRADFAESIDANAVHGSDAPETAAVEVAFFFPGMNVYSR"
     gene            complement(76778..78574)
                     /locus_tag="PLH85_01530"
     CDS             complement(76778..78574)
                     /locus_tag="PLH85_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB63265.1"
                     /translation="MSTYNAPIRDMQFVMKELAGLDEVAALPGCEEATPDLVDAILEE
                     AGKFAGGVLAPLNRIGDTQGPRWDNGVVHTSPGWKDAYTQFAEAGWTALACEPEFGGQ
                     GLPKLVSTAVMEMWKSANMAFSLCPMLTNGAIESIKLRGTDEQKAAYLPKMVSGEWTG
                     TMNLTEPQAGSDLAAVRTRAVPQGDGTYKIFGQKIFITYGEHDMTDNIIHLVLARLPD
                     APEGVKGISLFVVPKFLLNADGTPGTRNDVHCVSIEHKLGIHGSPTAVLAFGDNGGAI
                     GTLVGEANRGLEYMFIMMNEARFAVGMEGLALSERAYQQALGYAKDRIQGTDAGVRGG
                     PKVNILHHADVRRMLMVIKSTTEAMRALAYVVGAATDKAHYHADDAERARAQAFVDLL
                     IPVVKGWLTENSVELTSLGVQVHGGMGFIEETGAAQHFRDARITPIYEGTTAIQANDL
                     IGRKIAKEGGQTVKAVVAEMRQVEAQLAEQAGDEFAAIRKSLSAGITAVEEAVAYILA
                     TFGQDIKAASVGSVPFLKLLGIVTGGWQMGRAALVSARRLAEGSGDATFYKTKIVTAR
                     FYADHVLSQAPGLAYAVVNGAAGALALEEDQF"
     gene            complement(78633..79298)
                     /locus_tag="PLH85_01535"
     CDS             complement(78633..79298)
                     /locus_tag="PLH85_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNB63266.1"
                     /translation="MNLAADLFPLGWHVVAILGGLGVGLWVVRTGPWKRLLDGHQLNA
                     VLGATVILALLWSINAGVRPGLNLHLVGGMAVTLIFGPQLALLVLALTLGAITLNGSI
                     AWSAYPINLLLMVIIPVALATLAFRLVERVLPKHFFIYIFVNAFLCAALVVLAQGTLA
                     SASLFLAGAYEADVLLTEYLPFFLLLGFSEAWLSGMALTLLVIYRPEWVASFDDRSYL
                     MNK"
BASE COUNT        13669 a        27577 c        25112 g        13029 t
ORIGIN      
        1 acgtgcagga tatgcgccac gatcccctca acccgcccgc gcgccccggc gcagctggga
       61 cttcagggcg aacacccaat cccagaatgc ccgttgcgca gcgcactcat cgatgggtac
      121 ctgaacgctg ccgtccacca ccacttcgca gccttcaccg ccgtcaatgg gtgcttggga
      181 acatatcaat ctcagacgaa ttttgtaatg gggaatcagg tagacgagga aattcacgct
      241 caagactcag gagtgcgccg ccgcaacggc cgattcaaca gccccgatcc gaaacgcaga
      301 cgggagacgc atagacggga aatgggtcca catcggcgag ccgggcagcc cacgccccat
      361 ggccggaccg ggaaggccac gcacggcgca aggggttgat atcgcaccgc atcttactcg
      421 aaaaaggctc gtgaccgcga tgggcggctg cggctaggcc tgcccacgcg cccgttggca
      481 ggccgccgcc gtgacgacgc ccggtgctat cattcagcgg ttttggcgcc ggccggcgca
      541 ttcgacggcc cgccgcatcc ttccgcgccc cgaccccgcg aatatccgcg cccgcagggc
      601 aggaaggttc accgccacgg cccgcttcat gaatgccctg ccctccgtct cccgcccgtt
      661 ttccgaccgc gccgaaaccg tcgccctgtg cctgatcgtc gtctgcgcct tcgtcgtccc
      721 cctgcccacg gcgctgatca gcatctgcac cttcctgttc ctgctggcct ggctggcggc
      781 cggccggctg ggcgagcgct gggccaccct acaccgccat ccccagggtc ccctgtgtct
      841 cgcactgctc acctggatgg gcgtctccat cgcttggagc ccggcccctt tccacgaagc
      901 cctcgataac tggtggcact tccgcgaata catcctgttc ctgctgatgc tcaccgtcgt
      961 cgccagggaa ccgaaaaaat gggaacggcg catcctgctc ggctacctgg ccggctactg
     1021 cgtatccctg ctcacctcct acctgcgctg gttcgaggtg attcctctct acgaccaggt
     1081 gggtgaattt gccggtttcg gcggccacac gggcttcagc ctgatgctcg cgatcacgtc
     1141 ctacgcctgc atctggctgt ggcgggcgcg tcccggcctg cgggtgttct ggctgctgct
     1201 gatgatcctg tccctgttca acctgtattt catcaacact ggccgcaccg gtcaggtggc
     1261 cttcctggcg ctgattcccc tcatcgtgtt ccgctggatg ggcctgccgg ggctgatcgc
     1321 cggcgccctc ggcgcggtgg ccttcgccgg ttcgctctac ttcctgtcgc ccacggtgca
     1381 ttaccgggtg aacaacgccg tctaccagat cgagcagtac cgggccggca acaccaccac
     1441 cagcgacggc ctgcgcatcg agttctgggt caacgcggtc aaactcatcc gccaccatcc
     1501 gatcctcggc ggtggctccg gcagcttcgc ggccgaatac gaagtcctcg ccgcagagca
     1561 gcacatgacc ggcgaccacg tggcggtcaa tccccacaac gaatacctgc tcatgtgggc
     1621 ccagtacggc ctgcccggcc tcggcctgtt cctggcactg tggctcagcc agtggcgccg
     1681 cgcctgccgc gaagcggaaa cccggcgcta cctcttccac ggcctgatcc tcgccatggc
     1741 ggtgggcgat ctcttcaatt ccctgatcct cgataacatg gaaggccact tctacatgct
     1801 ggtcagcgtg gccctgggcg ccgcctgggc ccgcgacgac cgcgccggcg gccgctaaag
     1861 gcgcgcggat ccggcgcggc ggcccgcccg tttccgctac actcgggcca tcccgtcctt
     1921 ccgagaccgc cagcatgtcc tacgcgccct acatcaagga gatcggccgc gggccgaaag
     1981 gctcccgtcc cctcaccgtc gaccaggccg aacagctctt cggcgacatg ctcgacggcc
     2041 gcgtccccga cctggaactg ggcgccatca tcctgtccat gcgcatcaag gccgagtccg
     2101 tcgacgagct gttcggcttc cagcgcgccc tggacgcccg cacggcccac ctcgccgtgc
     2161 cgcccggacc acgcctggtg gtgctgccca cctacaatgg cgcccgccgc caggccaacc
     2221 tgatgcccct tctggcccgc ctgctggccc gcgagggcgt gccggtcctg atccagggcc
     2281 gccacgattt cgaatcccgc gtcagccctt tcgaactgct cgccgccctg gacatccacc
     2341 cggcgaccag cctgtccgac gccaccgatc gcctcgccag ggatcgcctt gcctgcgtac
     2401 cgctcgacct gctcgcaccc ggcctcgtgc cgctcctcgc cacccgtgtg cgcctgggcg
     2461 tccgcaacag cgcccacacc ctcgccaaac tcctcgatcc cgcccccggc cacagcgtgc
     2521 gggtcgcctc catgacccat cccgagtacc tggagcgcat ccacgcccac ctggtggcca
     2581 ccggcgccac ggcgatgctg ctgcgcggca ccgaaggcga ggccttcgcc aacccccgcc
     2641 gccgcccgct gctggagcgc tttgccaacg gtgtcgccga cgtggctttt cccgccgagg
     2701 aaggcggcgc ccctccgctg cccggcgtca ccgacaaccc tgaggtggcc gccaacgccg
     2761 agctcatccg cgccatgctg gcgggcgccg tgccgatccc ctcgccgctc ctcgaccagg
     2821 tcgcggcctt gcgggatctc gcacacctgc cgcgcgcctg aggggcatgg gcgcactgtt
     2881 cgcccatgtc acctgcggcc ggacaagcgt ctatactggg atctccccgg cataggacgg
     2941 tgtcgccccg atgctgccgg ttgccgaccc tcacccctgg agacgcacca tgagcaagaa
     3001 ggccctctgc atcggcatca acgactaccc cggcaccggc agcgacctct ccggctgcgt
     3061 caacgacgcc aacgactggg ccacgaccct taccggccgc ggtttcaccg tcaccaagct
     3121 gctcgacgcc caggccacca aggccgccat ggtgagcgcc atgagcagtc tcatcgccgg
     3181 cgccgaaccg aacagcaccg tggtcatcac ctattccggc cacggcacct gggtacccga
     3241 ccagaacggc gatgaagccg atggtcgtga cgaaggcctg tgtccctggg acattgccca
     3301 gggcaaggtg ctcatcgacg acgaaatcgc catcctcttc gccaagcgcg ccaagggtgt
     3361 gcgcgtgctg ctcatcgccg acagctgcca ctccggctcg gtgacccgtg ccccggagga
     3421 cattcccgaa cccggcgaac cgaagccccg cttcctgccc ctctccctgt ggctcaagac
     3481 cggctacaac ccgcccgtcg tgccgcccaa gccacccgcc ggcacgctgg gcctcgaccg
     3541 ggccggtggt gacaccctgc tggccggctg caaggacgag gagttcagct gggacacctc
     3601 cttcgctggc cgccccaacg gcgccttcac ctactacgcc ctcaaggctc tgggcagcct
     3661 gccggccaac gccacctacg ccgactggtt caagaagatc cggacctacc tgccgtccag
     3721 cgcctgcccg cagaacccgc agatcttcgg gccggacggc gccgacgcct tccccatctt
     3781 ctcctgacct cccgcgggac ggctgcgacc gtcccgtacg catcgatcct ccctgcccga
     3841 ggaatccgcc atgccccgta cctaccggat ccgcggcgcc caggacgcat cgcgtcccgc
     3901 ggtcgccctg cccattgccg acacggccgc catctccgtc acgcatgtgg gccacctcct
     3961 gcccggcact gcccgggacg ccgccccgaa cgaactcgac atcccggccg acacggtggt
     4021 gcgggtcacc ctcgacaacg gcttcgtgct gtggtcacga gccgatgacc tgatccgtga
     4081 atacggccgc aaggcccttg cccgggacgg cggcgaaggc tgggaattcg gtcccctggt
     4141 gcctccccgc cggggcgccg gcgaacgcgg cctgctggga ctgggcatca aggtgctgga
     4201 cttcttcggc atccacgtga tgaacaagcc tgcggcggcg atcgcggcat ggctcgagga
     4261 caagcgcctc ggcggccatg cgccgggcgt gtaccgctgc cccctcgacg gccccttcgc
     4321 cctgtcgccc cttggcacaa aggagaccct tgcggccgca ccgggtccga gcctggtctt
     4381 cctccacggc acgggctcca gcacacaagg cagcttcagc gaactgtggg atcccaaaaa
     4441 tgccagtggc gccgttctgc gggcccgtct gaagggcgtc tacggtgacc gtgtctatgc
     4501 cctggagcac cgctccctca ccgaaagtcc gatccagaac gccctgcagc tcgtggagag
     4561 actcccggaa agcaccgagc tgcatctggt cagccactcc cgcggcgggc tcatcggcga
     4621 gctgctgtgc ctctccggcg tagacgccct cgccgaatcc ctctcgccga ccaccctcga
     4681 cgccctcttc aatccggacc ggatcatcgc cagacagctc ggcctcggcc ccctgggcga
     4741 acaggccctc gccgaccgca aggcggccta tgacgctgaa cgccgacgcc tgagcgacct
     4801 ggtgggcctg ctcgccgcca ggaagatacg cgtctcccgt ttcgtccgcg tcgcctgccc
     4861 ggcgcggggg accacgctgg cgtcgggacg cctcgaccgc tggctgtcca ccctcgactt
     4921 cctggccggg cgtgtcctcg gcgacggact cttcatcggc ggactcgact tcgtgctggc
     4981 cgtcgtccac gagcgcaccg accctcgcac gctgccgggt ctggaagcca tgatgccggg
     5041 ctccgccctg accagcctgc tcgcggcccc cggcctgacc acccgcgccg acctcaccgt
     5101 catcgcgggc gacatcgaag gcgattcgct gctgcagaac ctcaagctga tggcggtgga
     5161 ctggttctac gccgccgatc acgacctggt ggtcaatacc ggctccatgt ccggcggact
     5221 caaacgtccg cccggcggag cccgctaccg cctcgaccgg ggcagcgacg tcaatcactt
     5281 cgcctacttc cagaacaaga agagcgtcga atggctgctc gccggcctca cccgcgccga
     5341 tggcgccgac ggcggcttcc tgaccatcgc cagcgccccg gccgaagccc cccgctggcg
     5401 tgacgccctg gcccgcagcg ccaccgtgag cgcgccgcgg cccctcgccg tggtcctgcc
     5461 gggcaccatg ggcagccacc tcagcgtcgc gggcgaccat gtctggctga attacggggc
     5521 cctgctcctg ggcggcctca agaagctgcg ccgggacgca aacggcgtca cccccaccga
     5581 gctgatcgac cagttctacg gccccctgct ggagtttctc gcccgcagcc accgggtcga
     5641 atacgtcgct tacgactggc gcctgtccat ccgggaggcc gccggtcgcc tcgccgacac
     5701 cctggagcgc tgcctgcctc aggccgaacg ggacaaccag ccggttcatc tggtcgccca
     5761 ctccatgggc gggctggtgg tccgctgcat gatcgccgac ggcgcccgcg gcgcctctct
     5821 gtggcaacgc atcacacgcc tgccgggcag ccgcttcctg atgctcggca cacccaactt
     5881 cggatcctac gaagccatgc gctggctcac tggattcaat cccaccgagg ccaagctgtc
     5941 cctgctcgac ttcacccaga gcaccgacga catcgtcaac ctggtgcgcc gctaccccgg
     6001 cctgctggaa ctgttgccct ttggcaccca ggacccggat ttctccaccg tcgccccgtg
     6061 gcagtccatc aaggacaaga ccctcgcagc ctgggacgtc ccccagagcg acgacctgcg
     6121 catcgccgcg gccacctggc agtgcctgcg caatgcagca ccagaccccc gcttcatgcg
     6181 ctacgtggcc ggctcccaag ccgccaccgt caccgactac cagctcctcg ccgacgacga
     6241 cctgaccaat ccccgccagc acctggactt cattgccacg cgccgcggcg atggcaccgt
     6301 cacctgggcg tcgggcaccc tgcccggcgt gcccatgtgg ttcgtggacg atacgcccca
     6361 cgatgccctc tgcagcgcca cgcaggcctt cccggcctat ctggaaatcc ttgccagcgg
     6421 cacgacgaac cgcctcccgt ccgtcgcgga cgcggtcagc cgcggcgacg cgccggacga
     6481 acgcttcccg ctgcccgcca cgcctcccga ggacggcatt cccgacgaat ccggcctgcg
     6541 cggcttcggc ttcggcggcg gcctgcccgc agagaccccg aacgcagccc ctgccgcccc
     6601 ggtcatcgag gtcagcatcc gccacggtga tctgtcctac gcccgcttcc cggttctcgt
     6661 gggccattac cagggcgacc cgctggtcca cgccgaagcc accctcgacc gccagctcga
     6721 cggcgccctg agccgccggg tcaatctgga catctacccc ggacgtctcg acagtcacgc
     6781 cgtcttcttc cgcgagaacc cccgcctcaa gcccaccggc gccctcgtca tcggcctcgg
     6841 ccaggtgggc gccctcacgc ccggcctgct ggaacagatc gcgaccccgg cactcctcga
     6901 ttacgcgctg caggcggccc gctggccgga cgaacgcttc gggccggccg gcgcgccccg
     6961 ctcggccgcg gtctcctgcc tgctggtggg ctccggcgcc ggaggattcc cggtgcgcga
     7021 cagtatcgaa gccctgctgc gcgccgccgt gacggtcaac cgccgcctgg tggacgcccg
     7081 cctcgaaggc cggatcaccc tcgacaagct ggaattcctc gaactcttcg aggatgtcgc
     7141 catcggcgcc gccgaagccc tgaaccagat tctggccgac ggcgagttcg ccgcggccgt
     7201 cagctggccc cagcgatgca tcgaacccgg tcagggcggt cgccgccgga tccgcttcgg
     7261 cgacgccccc ggctggtggc accgccttga catcgcccgt gacggcgagc gcctgcgctt
     7321 caccttcgcc accgatcggg cccgcgccga ggagaccctc gcgaccggtc agctcgccct
     7381 ggccgacgcc ttcatccggc aggcctcgcg ctcgccccgg gccaatccgg aggcggcccg
     7441 caccctgttc gagctgctcc tgccccttcg cctgcgggag atggcgcccc agcagggcga
     7501 catggtcctg ctcgtcgatg aggattcggc acgctttccg tgggagctgc tggaagaccg
     7561 ctggagccac agcgggcgcc cgcccgcggt ccaggccggg ctgctgcgcc agctcaagac
     7621 cgccaccttc cgagcgcgcc ccagccttgg cagcgaagca cgcgcactgg tcatcggcaa
     7681 cccggacctg agcggcagca ccgtcttcag cgacctgccc ggggcacgcg acgaagcgac
     7741 ccgcgttgcc ggacttctgg gcacgcgcgg cttcgaaacg gaagactgca tcgacgccaa
     7801 ggccaccagc atcgttgaaa ccctgcacaa gcggccgtgg cggatccttc acctcgccgg
     7861 ccatggcgtc catcgcttca tgatcgccgg cgacacgccg cggccctgct ccacctgcgg
     7921 acaggccacg ccggccccac agaaaccgat ctccggaatg gtcatcggag acggcatctt
     7981 cctcacgccc ggcgacgtgg agcagttgcg ctgggtcccc gaactggtct tcatcaattg
     8041 ctgccacctg gggaacacca ccgcgcccgc ggacgatctg gaccgcgccg gcctcgccgc
     8101 caatctgggg gtcgagttca tccgcatggg cgtgcgcgcc gtcattgccg caggctgggc
     8161 ggtggacgat cgggcggcgt ccaccttcgc cgaaaccttc tatggacgca tgctcgccgg
     8221 cgaaagcttc ggcgatgccg tgcgtggcgc acgggagcaa tgctggatca gccatccggg
     8281 cgtaaacacc tggggcgcgt atcaatgcta tggcgacccg gcctatcgcc tgagacccgc
     8341 ggtcgccacc accgagacag ggcacacgcc cttctttgcc ccgtctgaac tggtggccga
     8401 actgagcaac ctcatcggcc agctcaaggc ccacgtggac ccggacagcc cgcgcatcat
     8461 cgaggccatc ctgcaacgca ttcctgccgc ccaaggcgca tcctggcgtg cccgcgccga
     8521 cgtgagcgcg gccctcgggc tggcctggag cgaagcccgc tgctggccgg aagccatcgc
     8581 cgcgctggaa caggcgatgc aggccaacag cgccgacctg ccggtgcgcg ccatcgagca
     8641 atgggccaac ctgagcgccc gccgagccgt cgagtgcctc catggccgtg cgccgatcac
     8701 cgctgcgcag cgcaaggaga tggtcgccac cctcaggacc gtgatcgacc gcctgcagtc
     8761 cctgcgcacc ttcggcgcca ccatcgaacg cctcagcctg ctcgggagca cctggaaacg
     8821 cctcgccctg gtgcagacgg gaagcgcccg cgtcgcggcc ctcgggcaca tggcagacgc
     8881 ctatggcgag gcctttgccc tcggcaaaga gaccgacccc tatccctttg caaatgcctg
     8941 gacggccgaa ctgctccagc ccgatgcgcc gtcccggcct gaacgggaca cgacctgcag
     9001 ccgcctgctc gccctgcagg cagacgccaa cgcccgcaac cccagcttct ggggcgacat
     9061 cgcggtggcc gatctgaagc tcgcccgact cctggcccgc tgcgcaggcg gcgggcccgc
     9121 cccgagactc ccggagacat gcctcgagct gagccaggaa gtcaccgaag cctatcaggc
     9181 cgcccttgcc cggggcgcca gcgaacggga gcgcgcgtcc gtgacggaac acatcgattt
     9241 cctgatcaga ctgaccgcac cgaaatcgcc ccagcttgcc ttcctccaga cactccgcaa
     9301 taccctcgcc tgagcgccgt catcagccgt ctccgtccgg tacgcgcccg gcggagacgg
     9361 caacaccccg cctctccatg caccgcaagg gtgcccgccc ctgcgtgcac cgcaccatga
     9421 aggatcattt cgccccgcaa cagtgcgcac agtggcaaag acactgctgt tttttcagcg
     9481 aaaacttttg gcataagcat tgcttcagct ccgtgacaag gagctcgcat ggctgaaacc
     9541 aaatccacct gttgttactg cggcgtcggt tgcggcgtca tcatcgaaca cgacgccgga
     9601 cgcatcaccg gcgtacgggg agaccccgac cacccggcca acttcggccg cctgtgcatc
     9661 aagggctcca cgctgcacct cacggcggca cctgaaaccc tcgccacgcg cgccctccat
     9721 cccgagctcc ggaccggccg cgcgctgccc cgccagcgcg tcagctggga caccgccctc
     9781 gacaccgcgg ccgaacgctt tgcgaagatc atcgccgaac acggccccga cgcggtcgcc
     9841 gtgtacggcg ccggccagct cctcaccgag gactactacg tcttcaacaa gctggtgaaa
     9901 ggcctgatcg gcaccaacaa cctggacacc aactcgcgcc tgtgcatgtc cagtgcggtg
     9961 gccggctaca agctcagcct gggtgccgat gcgccgccgt gcagctacga ggacatcgcc
    10021 cacaccagca ccctcttcat caccggcagc aacaccgcct tcgcccaccc catcgtcttc
    10081 cgccgcgtcg aggatgcccg caaggccaac cccgacatga agctggtggt gatcgacccc
    10141 cgccgcaccg acaccgccga aggggcggac ctgcatctgg ccctgctgcc cggcaccgac
    10201 atcgccctct ataacgcgat gctccacgtg atgctgtggg aaggctgggt gaatcgtgac
    10261 tacatcgacg cgcacaccga aggcttcgac gccctcaagc gcaccgtccg cgaatacaca
    10321 cccgccatgg ccgccaccat ctgcggtctg gacaagaagg tcatcgaaca ggccgccgaa
    10381 ctcttcgcca cctccccggc caccctgtcc atgtactgcc aggggctcaa ccagttctcc
    10441 ttcggcaccc acaacaactc ggcgctgatc aacctgcacc tggccaccgg ccagatcggg
    10501 cgtcccggcg ccggcccctt ctccctcacc ggtcagccca atgccatggg cggccgcgaa
    10561 gtcggcggtc tggccaatct gctgtcggcc caccgggaca tggccaaccc gcagcaccgc
    10621 gccgaagtgg ccgccctgtg gggcgtggac agcgtgccgg aaaagcctgg caagagcgcc
    10681 gtggagctgt tccgcgccct caagagcggt gaagtcaagg ctgtctggat cgcctgcacc
    10741 aacccggccc agtccctgcc tgaccagacc ctggtccatg aagccctgca gagcgccgaa
    10801 ttcgtcatcg tgcaggaagc cttcggccac accgagacct gcgcctacgc cgatctcctc
    10861 ctgcctgcca gcacctgggg cgagaaggac ggcaccgtca ccaactccga ccggaccatc
    10921 tcccgcgtcc ggcccgccgt gtccgcgccg ggcgaagccc gccacgattg ggcgatcgtg
    10981 gtggatttcg cccggcgtct gggcacccgc ctcggcaagg gcgaccttgc cgcgcggatg
    11041 ttcccctacg accggcccga agacgtctgg aacgagcacc gcgaatccac ccgcggtcgc
    11101 gatctggaca tcaccggcct cagctacacc acgctggaag acgtcggtcc ccagcagtgg
    11161 cccttcatcg aaggcgccca ggctggcaag gtgcgcctct acgcggacgg cgtgttcccc
    11221 acgccatccg gcaaggctcg ctttgtcgcc accgaatacc gagtcaccgc cgaggcgccc
    11281 agcgcccgct tcccgctgca cctgaacacc ggccgcctgc gcgaccagtg gcacggcatg
    11341 agtcgtaccg gcctggtggc ccgcctctac agtcacgaat ccgaacccct gctgcacatg
    11401 cacgccgacg atctcgcccg ccgccagctg agcagcggcg atctggtccg tgtgaagagc
    11461 cgccgcggcg cgattgtcgt caaggttctg gaatccgaca ccgtgcgtcc ggggcaatgc
    11521 ttcctgccca tgcactgggg cgccaacgcc atgggcggcc agggcgtgaa cgtcctcatg
    11581 ccgcccgact tcgatccgac ctccaagcag cccgagctca agcatgccgc cgtgcaggtg
    11641 gagaaattca ccgaaggcaa ggccctcgtg gccttgcgcc gctccgaacc ggatgcgaac
    11701 ggcgtcctgc agccgaatcc gctcgaagtc atggagagcc tgcgccgctg gctgccgcac
    11761 ttcccctatg ccagcctgac cctggccgga cgccatgagc cggtcgtcgt gctcaaggcc
    11821 cgcggcgagg aactcgcgcc ggaactcctc gaggccctgg accgggatct gagcctcgac
    11881 gatccgaccc gcgtcctgtc ctatcaggat cgtcgcaagg acgtggccaa gcgggcactg
    11941 gtggagggcg actccctcac agccgtccgt ctggcgggcg agacccgcgc ggcagactgg
    12001 ctcgccgaca tgatggtgcg tggccactcg gccgccgcgg tgcgcccgtg gctgctcgcc
    12061 ccggtggatc agccgccagc cggccagtcg gcgcgtggca aggtcatctg caactgcttc
    12121 gacgtatcgg aagaggacat cgtggccgcc ttcaagaatg gcgacaccct cgacgccctc
    12181 caggcccgca cccgctgcgg caccaactgc ggctcctgcc tgcccgagct caagcgactt
    12241 cagcagcaga acagccactg agccgggcct cgcgcacccc acgaaaaaac cgggcctgaa
    12301 ggcccggttt tcattgccca acccgggcgc ctaggacgcc ggcgcccacc cctgggccac
    12361 cagccactcg aaggtcgcgg cggacaccgc ggggcaatcg tccggcccgg aaaatgtgtg
    12421 atcagcctgt tcaaaatgcc gcgtggacac ctcgggccgt gtcacgacgg cttgccagcg
    12481 ttcgctgccg tcacgcacct gctcgaattc ccgcgcaacg taatcccgac cgctgaggaa
    12541 tagcgcggcc ggcgtcccgg cgcgtcgcag catgccctgc gccatgcgct cgggcaggct
    12601 gccggcgtcg gcctcatccg acgaattcgc cgtcctggcg gccccgtcac cgcgcgccgc
    12661 ccgcgcggtg gaaaacaggg accggatcga tccgaacaca ttgacgccgc cccccagcag
    12721 cttcttccag aaggacggat cggacagacg ctgcaggtaa tagtgcttca ggaacacctt
    12781 ggcctgcccc gcactggtct gcacccaggg gttgaggatc accacgccgg ccacccgcgc
    12841 atcgctcgcc ggatagacgc acactgcgct ggccccgtca cacaggcccc acaggacgac
    12901 cttctcgagc cccggttggg ccgcaagaaa ggcgtccatg gcggcccgaa tgtcctcgct
    12961 cacgtccagg aagtggcgcg ccgcgccgct ggcatcgccc attccgcgat aatcgaagcg
    13021 cagacagggc accccgcgcg ccgccagata gcgcgccaga ctgacgaact ggcgatggct
    13081 gccgacccgg tactggggcc cgccgaccac gaccagcacc ccgacgcgag ccggcccctg
    13141 cggcgccgaa aggatcccga ccagagactc tccgccgcac tcgaagacga gcgcccgatc
    13201 ctgccagttc atcgcacgaa ccccttcaac gcctcgaccg aggcctcgat aagcggctcg
    13261 cagacttcga tttcctgggt ctgccagaag gcaacacccg gcaccaccgc cgcacgcgct
    13321 gccgtccccg cgttcgccca gcgatcgacc ggcccccgca gcgcgggtga cacctcctga
    13381 cgatccgccg gcgccacctc cagcgccacc accggcgccg catacgcggc tggcaacgac
    13441 agcgtcgccg cctccatgcc ggaggccagt gcggccggca ggcgataccc ggcaacatcc
    13501 accacgccgc cggcctttaa tgtggcgcga atggcgtcca tggaagcctt ggcgtcggca
    13561 tcggcgagca tgtcgaaggc ggccttcagg cgcaggaact gggtcaggtg ctgcttgccg
    13621 ttgccgaccg gctgccacag cagcagcggt ggcgccgtgc cgtccgcctt gatccagtcg
    13681 gacaccacca gggccccgag acgcatgccc cacagggcca ccggcacccg caggtctgtc
    13741 gtcgcccagc gcacggcgtc gcgcagatcg tcgatccacg taccccatga cacatcggca
    13801 agctcacccc cgctgtcgcc gcagccggac agatccattt gcaggacgtt ccagccccgc
    13861 tcggcgaagg cgcgcgccgc gagcgccacc atccgccggg acttgttcat ttcctcggcg
    13921 aaggcgtgca cgaatacaag ggatcctacc ggctcgggcg cgcgcgtaag gaggcagaat
    13981 cgtcctccgt gcgcagcccc cgggaggaag aacgcctccc gccgcaacgg ctgcacgccc
    14041 tgacctcagg cgagcttggc gtcgacgaac tccaccagcg agccgacgga ggcgaaggta
    14101 gcgccgtcga tctcatcgtc ttccacgaca aagccgaaac gctcttcgag catgttgatc
    14161 aggcccacga cggccatgga gtccagatcg ggcagagccc ccagcagcgg cgtatcgaga
    14221 tcaaactccg ccgcacgccc tcccaggctc aacacctcgt cgagaatcga cagcacttcc
    14281 ttcttggtat ccaaaagcag actccgtagc aaaactaaat cacatcgcag cggccgggcc
    14341 gagcgacggc gtcatggcaa gactgctatc attatattcg acactgcggt cctcgggacc
    14401 gtgattgaag ttgcaggccg cgtcggcggc attttccggt tcggccggtt tttttttgcc
    14461 cagcgatgaa acgcgttctc ctgatcgcct tccactttcc acccgtccag ggcagcagcg
    14521 gggtacagcg gaccctcagg tttgcgcgct acctgcccga tttcggctgg gaacccatcg
    14581 ttctcacggc ccacccgcgc gcctacgcgg agacctccga cgatcagctc aaggacattc
    14641 cccccggggt acgcgtcatc cgcgcccccg cctgggacag ctcccgccac ctgtccatcg
    14701 ccggccgcta tccgggcttc ctggcgcgac ccgaccgatg gatgtcctgg tggctcggcg
    14761 cggtaccggc cggcctgaaa atcatccgtg aattcaatcc actggcgatc tggtccacct
    14821 accccatcgc caccgcccac cgcatcggcg gcagcctcgc gcgcctcagc gccctgccct
    14881 ggatcgccga ctttcgcgat cccatggtgg acggcgacta cccgtccgat ccggccatcc
    14941 ggcgcagttt cgaggccatc cagggcaacg ccatccgccg cgcggcgtgc agcaccttca
    15001 ccacccccag cgccctggcc atgttccgcc gccagtttcc cgagcgggcg aaccacatgg
    15061 ccctgctcga aaacggctac gacgaagaga ccttcgccgg ccagaccgcc accggacgcc
    15121 tccatcccga acgcctcacg ctgctccaca gtggcgtgat ctacccctcc gaacgggatc
    15181 cgacacaatt gtttgccgcc ctgcgcctgc tcaaggagcg cgcaccggcc gtcctcgccc
    15241 gcctcggcgt gcgcttccgg gcgcccatcc atttcgacct gctcgccgat ctggcgcgga
    15301 cctacggcgt cgaggaacac gtggagctcg cccagcccgt accctatcgc gaagccctgg
    15361 ccgagatgct caacgccgac ggcctgctca tcctgcaggc cgccaactgc aacgcccagg
    15421 tgccggccaa gctctacgaa tacctgcgcg cgcgccgccc ggtcctgacc ctgaccgacc
    15481 cggccggcga caccgcacag gtggtccgcg acgccggcat cggtgccctc gcccgtctgg
    15541 acgacgcggc cgacatcgcc agcctgctcg aacgcttcgt cgcccagccc gacgccggca
    15601 ctctgcccac cgaagccgcc atccgcggtg cctcccggcg cgggcggacc gaagccctcg
    15661 ccggcctcct cgacagggcc gtcgcccacg gcggagcctg agcgatggcg ctgggcaagc
    15721 tgcgcgccac ctttgccgag atgggcgccc tcaacggcgg tctctatctc ctccaccgca
    15781 tcctgcagac cctgagcggc ggccgcgcac agctggtgcg ctactacctg gtggcacaac
    15841 cgatccatcc ggacgccgcc tgccgcccgt cggccaagaa cccggtcacg ctgctcgacc
    15901 cttccgatcc gctgattgca gcctttccac gtccgccgga ggtcattgcc aaacgcctgc
    15961 gggacggcgc cacctgcttc atcgcccgct ccggcgagac cttcgccggc ttcctgtggc
    16021 tcgcccatgg cgcctacgac gaggacgagg tgcgctgccg ctacgaactc gtggacgacg
    16081 accgctgcgt gtgggattac gacgtctacg tcgaacccgc ctaccgcatc ggccggacct
    16141 tcgcgcgcct gtgggatgcg gccaaccagc acctcgcccc ccgcggcgtg cgctggagca
    16201 tctcgcggat ctccgccttc aatgccgcct cgctggccgc ccacgaccgc ctcgggatca
    16261 agattttgca cagtgccacg ttcatctgcc tcggatccgt gcaaattagc gccctcggcg
    16321 gcgcgcccta tctgcatatc ggcatgtcgg acgcgtcccg gccggtcctg cgcctgcgcg
    16381 caccggacgc tgagtaggcc ccgtcaggca gtttttcgca attccgccgc acaggctcgg
    16441 atacactcac cgcttcattc aaagaacatc ctctggccct caccgaccca tgaacgccac
    16501 cacccttctt caccagctcg tcaccgaatc cgccttgcgg gcgcccgatg ccggcgcgct
    16561 gcgtgacggc gccgccgaac tgagctacga agcccttgcc ggccagatcg gcgcctttgc
    16621 ctccggcatg atggccctcg gcctggatcg caccgaacgg gtcggcgtgt acctggacaa
    16681 gcgcttcgag accgtcatcg cgtcctttgg cgccgccgcg gccggtggcg tgttcgtgcc
    16741 ggtcaatccc atcctcaagc cccagcaggt cggctacatc ctgcaggact gcaatgtacg
    16801 ggtgctggtc acctccgtcg agcgcttcaa acccctcgcc gataccctca gcgactgcca
    16861 cgacctgcgc cacgtgatcc tcaccggcaa ggtggacacc ctgcccgaac tgcccggcat
    16921 gtccatccac cgctgggccg acgtgatggc tgcgcccgcc cgcgccggtc accgggtgat
    16981 cgacaacgac atgctggcga tcctgtacac ctccggcagc accggccgtc ccaagggcgt
    17041 cgtgctgtcc caccgcaaca tggtggccgg tgccaagagc gttgccacct atctcgagaa
    17101 ccgcgccgac gataccctcc tcgccgccct gcccctgtcc ttcgacgcgg gcttttccca
    17161 gctcaccacc gccttccacg ccggcgcccg ggtcgtcctc ctcaactacc tgatgccccg
    17221 cgacgtgctc aacgccctcg tcaagcacaa ggtcaccggc ctcactgccg tgccgcccct
    17281 gtggatccag ctctcccaac tcgagtggcc cgagaccgtc accgaccacc tgcgctacat
    17341 cgccaacacc ggcggacgca tgccgctgga aaccctcacc gccctgcgtg cccgtctgcc
    17401 gcggaccaag ccctacctca tgtatggcct gaccgaagcc ttccgcgcca cctacctgcc
    17461 gccagaccag gtcgatgtgc gtcccgactc catcggcaag gccatcccca actccgacgt
    17521 cttcgtcctg cgcgaggacg gcagcccctg tgaaccgggc gaacccggcg aactcgtgca
    17581 gcgcggcgcc ctcgtcgcca tgggctactg gaacgatccg gaaaagaccg ccgaacgctt
    17641 caagcccctg cccgtcaccg cgccgggccg ccaggccggg ctggtgctgc ccgaaatcgc
    17701 cgtgttctcc ggcgacaacg tgcgcaggga cgaggaaggc tacctgtact tcatcgggcg
    17761 ccgcgacgag atgatgaaga cctccggcta ccgggtcagt cccaccgaag tcgaagagat
    17821 cctctacggc acgcacctgg tgggcgaatg cgtcgccttc ggcgtgcccc atcccagcct
    17881 cggccaggcc atctgcgtga tcgccacgcc gcgcgccggc agcgccctcg attccgccgc
    17941 cctgctcgcc gaatgcaagc agcgcatgcc cgcctacatg gtgccctcgg tcatcgaagt
    18001 gcgcgacggc ccgctgccgc gcaaccccaa tggcaagatc gaccgcaaga ccctgtccac
    18061 cgaattcgaa aacgcccgta aggtccagtc atgagcgaca ccccccgcga aaagcccgtc
    18121 cacgccccga tggaccagtt cccggtcgtg aacggcgaac tcaccgtggg cggcatttcc
    18181 ctcactcgcc tggccgaccg ggtcggtcag acccccttct acgcctacga ccgcgccctc
    18241 ctcacccgcc gcgtacagga cctgcgcgcc acgctgccca cggccatcaa gctccactac
    18301 gccatgaagg ccaaccccat gccggcggtg gtggatcaca tggcgcgcct cgtggatggc
    18361 cttgacgtgg cctccgggcg cgagctgcgt gtcgccctcg acagcggcgc caatccccat
    18421 gaaatcagct ttgccggccc cggcaagagc caccaggaac tgcgccaggc cgtcgctgcg
    18481 ggcatcctga tcaacatcga atccttccgc gaagtggccg aactggccgc catccgccag
    18541 gccaccggct ggcatgtccg cgtggccgtg cgcgtcaatc cggatttcga actcaagagc
    18601 tccggcatga agatgggcgg tggccccaag cagttcggcg tggacgccga gcaggtgccg
    18661 gaactcctcg ccgaaatcgg ccgctccggc ctggccttcg agggcttcca cctcttcgcc
    18721 ggatcccaga atctgcgcgc cgaatcgatc gtcgaggccc agcgcaagtg cttcgacctg
    18781 gccctgcgcc tggccgaatc cgcaccgtcg ccggtcaggt tcctcaacct gggcggcggc
    18841 ttcggcattc cttattttcc gggcgaacaa cgcctcgacc tggcccccat cgccgccaac
    18901 ctggcgatca tcgccgagga agccgcggtc aaactgcccg gcgccgagct ggtcatcgaa
    18961 ctggggcgtt atttcgtggg tgaggccggg ttgtacgtct gcaaggtggt ggaccgcaag
    19021 gtctcccgtg gccacaccta cctggtcacc gacggcggcc tgcatcacca cctgtccgcc
    19081 tccggcaact tcggccaggt catccgcaag aactacccgg ccgccgtcgg caaccgcatg
    19141 gacgccccgc tcggcgaagc ggtgtcggtg gtcggtccgc tgtgcacgcc tctcgacctg
    19201 ctggccgacc ggatggcgct gccggaagcc cgtccgggcg acctgatcgc cctttaccag
    19261 tccggcgctt acggcgccac cgccagcccg cggggcttcc tgagccatcc ggatctggtg
    19321 gaggtgctgg tctgacgtgg catccgaact gcaccacgcc gcaccgtctc gtgccgcccg
    19381 ccggccggct gaacgggttg cggaccccgg accagccatg gccggtcctg cgcccggtcg
    19441 tgaccggaag atcccgatgg atctgagttc ttcaaccgcc gtcccccacg ccgagccttc
    19501 tgcggaccga aggccaccgc ccacccggtg gcatcgggcc acggccgcat tcgcgcactg
    19561 ttgagttgtc ttccaagatg gacgccaccg acgcctccct gcctgccccc ctcatcacga
    19621 cgatcatccc cacgatggcg tcaaaggcgc gggccgcctc gctgaagcgg gcgatcacct
    19681 cgatccgcgc ggcatcgaag cagcccgttg ccatcatcgt ggtggtgaac gggacccgca
    19741 gcgatcccga ggcctgcgcc tggctggccg atcaacctga cgtgcgcctg gagttcgacg
    19801 ccaagccatc tgcggccaac gccatgctgc gggggcgcga actggtggcg acgcccttct
    19861 tcacgtcgct cgacgacgac gacgaattca ccccgggcgc gctcgacctg cgcctggccg
    19921 cgatgcagtc cgacacctgc cccgacatcg tggtctgcaa cggcctgcgc cgtgacagca
    19981 cgggcgacac cctctgctac gacgatctac cgagcgtgcc ggcggcgccc ttgccggccc
    20041 tgttccgcat gccatggctg tccagctgta attccctgta ccgcacggcc agcatcgatg
    20101 cgcatttctt ccgcaacttc cactcctttg ccgagtggac ctggctcgcc tatcagttcg
    20161 gcgtggcggg caagaagatt gccgtaatcg atacccccac attcgtgtgc aacgacactg
    20221 cgggctccct ctcgaaatcg aacgagtacg agatggccta cctgtcgctg tacgaacgga
    20281 tgctgagcct ggcaccgccg gctccggtgg cggatctcat ccggcggcgg atcggctcat
    20341 tttggcacga ccaatccgtt actgccctgc gcaacggcca gcgcctcgac gccctgcgcc
    20401 accacgtgcg cagcctgcgc cagcccggcg gcctgcgcta cctgggctac tcccgttacc
    20461 tgatccccgg ctggccccag aaccgatgac ggactcctct tccaacagcg tcttcaaggg
    20521 cgccctcatg accgtcctca tgcgctggac ggaccgtctc atcggcttca tcagcaccct
    20581 gatcctggca cgcgtgttgg tgcccgagga tttcggggtg atcgccatgg cgtccctcgt
    20641 ggtcgggctg ctcgacgtgc tgctggatct gggggtcaac gtcgccctca tccagaaccg
    20701 aacgccgagt caggctcatt acgactccgc ctggaccctg cggcttgccc aggctgccac
    20761 caccgccctg ctcatcgtca tcgcctcacc cttcgccggc gattacttca acgatccgcg
    20821 cgtcacgccg gtgctgcagt tcatggccct gagcctcttc gttgccggcc tggagaacat
    20881 cggcaccgtc accttccaga aggacatgcg cttcgacctg gaataccgct tcaatttctc
    20941 caagcgcatc gtcgccttcc tggtcaccgt caccagtgca tggttgctgc acagctactg
    21001 ggcgctggtg ttcggcaccc tttccggacg gatcttcggc gtcttcctga gctaccgcat
    21061 ggtggccatg cgtccgcgcc tgagcaccga gaagatgcgg gagatcttcg ccgtctccca
    21121 gtggatgctc gtgaacagca tcggcatcta cctgaacgcc aatctgcaca agatgttcgt
    21181 cgggcgcttc gcctccaccg gcacgatggg ggggtacacg ctggccgacg aaatctccgc
    21241 gatgccctcc accgaaatcc tggcgcccct caaccgcgtg ctcttccccg ccttcgtgcg
    21301 ggccaagcac gacctggtcg agctgaaacg ggtcttcctg ctggcccagg gagttcaatg
    21361 cctgatcgcc gtgccggcca gcctgggcct ggccttcatc gcccacgacg ccgtgctggt
    21421 gctgctgggc gaacgctgga gcctcgcgat tcccttcgtg caggtgctgg ccctttccaa
    21481 cgtggtccag gccatcacca acagcggcgc ctacatgctc ctcaccctgg gcaaggtccg
    21541 cagcgtgacc ctcaccacct gggcccaggt catcctggtg gcgatcggcg cgctgctcgt
    21601 gctgcatcag ccggaagccc tcgacatcgc catcctgcgt gtcagcgccg tcttcgccgg
    21661 actcgtgctg gccatgtgga tcctcatgct caacatgagc gggctgcgcc tgctcgaact
    21721 ggtgcgcacc atcgtgcggc ccatcctcgc cacggcggtg atgggcggcg ccatccacgg
    21781 gctgggcgtg ctcaccgccg atctcccggt ggtcgtcgcc atgttcctca agatctttgc
    21841 cggcatgttc agctacacgg ccgcggtggt gctgatctgg cgcctggccg gacgtcccga
    21901 cggcgccgaa tcctatgtct tcgacaagct cgccggcgtc ctcaggcgcc gcaaggccgg
    21961 gaccgcctga cccctcttcc ccattcctgg aacgcactga cttacgaggt ttgttttgga
    22021 caaccgattc tccattcagg ggcgcctcag cccgaatccg gccggcacgc cgggcgaact
    22081 gtccatcgag cgcctggacg acgggtggcg ctgccactgg gccggcgccc gcctggaggt
    22141 ccatgcccct gcccgtctgg cggagcggga cggcatcctg tgcgtggcca gcggatcgcc
    22201 ccgcctgccg gtcagcggcg ccgacatcgc cgacgcctgg ctcgaactcc acgcccgcca
    22261 tggtgccgat gtccatcgcg acgtgaaggg cctgttcagc gtcgcgttct ttgacacccg
    22321 ccgccagacc gcatcctttt acaccgaccg ctttgccacc gaatcctggt gcttcggtga
    22381 agaagacggc gccctgtgct tttcggaccg ggccgacgcg gtgcccctga cgcgtcgcag
    22441 cgtcagtgcc cagtcgatct tcgactacct gttcttccac gtcatcccgt cgccggtcac
    22501 gctcttccgg gaagtccgcc gcctgccccc cgcaagccgc ctcgactggc agaacggcag
    22561 cacccgtcag gccccctact gggtgccggt cttccgggag gacacgcgtg tgtccgtgga
    22621 ggacgccaag gcccgcttca tggagatcgt gcgcgactcc gtcacccgcg agatcgaccg
    22681 ggacgacgtg ggcgcatttc tctccggcgg caccgacagt tccaccgtct cgggcatgct
    22741 cgggcgcatc accggccggc cggcgcggac ctactcgatg ggcttcgacg ccaccggcta
    22801 cgacgagatg gagtacgcgc gcatcgccgc caagcacttc aagaccgatc accacgagta
    22861 ctacgtcacc ccggaagatc tcctcgaagg cattcccaag gtggcacgca gctacgacca
    22921 acccttcggc aactcctcgg ccgtgcccgc ctggatctgc gccagccgcg cccgcgccga
    22981 cggcatcggc aagctgctgg ccggcgacgg cggtgacgag ctcttcggcg gcaacgcccg
    23041 ctacgccaaa cagcgcgtct tcggctggta cgacaccatc ccctcgcccc tgcgcagcgg
    23101 cctgatcgaa cccctcagcg gcctgcccgg catggaccgc atccccctta tcaagaaggg
    23161 cgtcagctac gtggagcagg ccagcgtgcc catgcccgac cgcatgcaga tgtacaacct
    23221 gctcctgcgc ctgggcctcg acaccgtgtt ccagcccaac ttcctcaagg gcatcgacac
    23281 cggcgagccg tcccgcctgc agcgtgacac ctgggccctg gtgccggaag gcagcctgct
    23341 caaccgcatg ctcgccttcg actggaaata caccctcgcc gacaacgacc tgcccaaggt
    23401 catcggcacc acccgcctgg ccggcgtgga cgtgggcttc ccgctgctgt cggacgagct
    23461 cctcgacttt tccctgaccc tgcccagcga atggaagctc aagggcttca agctgcgctg
    23521 gctgttcaag gaggccctgc gcggcttcct gccggacgag atcatcacca agaagaagca
    23581 cggcttcggc ctgcccttcg gcgtatgggc cgtcaagcac cccggcctca aggccctcgc
    23641 ctgcgacacc ctggaggcct tcggccagcg cggcgtgatg agccccacct tcctccacga
    23701 cctgcacgcc aagtggctgc ccgaacatcc gggctactac ggtgagctgg tgtggatcgt
    23761 catgatgctc gaacagtggc tgcaggccta cgcgcccgac tggcgcctcg gcggcccggc
    23821 ccgggcatga cggccgcccg ccccgacgca ccgtctgcag cctctgcaag ccgcccaccc
    23881 gcccgtgcgg gcgcggcgct gctgcggctg ggcctgccgc tgctcctcat cgccctggtg
    23941 tggctggctc actatgccca tttccacgtg gaccgcagtc cggtggcaga agccgtggtc
    24001 gagcaggccc gtcaggcgcc cgacgacgcc cgcctcgacg agatcggcca catgtccctg
    24061 gccatcgacc tgggcgtccc gtcggaccgg gtcgtggcca ccgccgatct gatcgtgcgg
    24121 ggcaccgccc tctttcccgc ctacctggat ggcccgacgc cgctgaccgg atatccggcg
    24181 gatttccgct tcggtcccgg taccttccag ctcgctgccg ccggattcgg ctttgaagac
    24241 atcctgctgg aagcctacga acgcaccgcc gacgcccgct acctgcgcac cgcctaccag
    24301 cgtattcagg atttcgccgc cttcgagctc acccagcggg aagccgtcga tttcctgtgg
    24361 aacgaccacg ccatcgcctg ccgcgttgcc gtggtggcgc gcctgtggcg ccacatccgc
    24421 cacgacccgg cctaccccgc cgacggtctg cgcaacctgc tgtccctcat cgcccgtggc
    24481 ggtcgctttc tcgccgcgcc ggaccacttc accgcacgca ccaaccacgg ggtgatccag
    24541 aacgtcgccc tcatgcagat cgccgcggcc ttcccgagcc tgcccgacag cgccggctgg
    24601 gcccgcctgg gcatggagcg gctggccctg caactgccct tctacgtatc gcccgaaggt
    24661 gtcgtgctgg aacactcggc ggattatcag cacgtgggcg tcagcctgct cgccatggcc
    24721 gtccgcctcg cccgcctcaa cgacctgccg gtttcccgcg agctgctcga caagtctgcc
    24781 gccagccgga ccgtccagag ccagctccag cgccccgacg gcagtctgcc cgccgtcggc
    24841 gactccatcg tgggacgccc ccacaccgcc gtcaccggcg ccaccgacgg ctccacaccc
    24901 gtcgccgaac agccggcccc gttcccgctc cctgagcccg ctgcccacgt ctatccggtc
    24961 tccggctacg ccctgtggtg gaccggagac gatccagccc tgcgcagcca gaccgtcatc
    25021 gcctgggcca aacacgacgg ccacggtcac aagcacgccg acgaaggctc gctgctcctg
    25081 tggtccggcg gccgtgactg gctcaccagc atcgcctact ggccctacgg tggtgccaac
    25141 gaaaaggctg cctacggctg gtccggcgcc aatgcccccc acggtatcgg tgagcttgcc
    25201 gccagcgtgc gcaccgtgga actccttggc agcgccgacg acgcccagat gcacgccgtg
    25261 gacctgctcc gccgcaccgg cgacggcgcc caattccggc gccagatcgt ccagatcgac
    25321 gcccagacct ggctcgtgct ggatttcgtt gacggaagcg catcgggcag cgaaaccgac
    25381 tggaccctgc cgccggcatt gggcctcacc gcccgcgccg acgaccgcag cttccagtcc
    25441 acagaagccg aggggcgccg gctgatcctc agcctggcgg cgccctccac gacgcgcagc
    25501 cgcatcctgc gaggctccca ggaccccttc gcaggctggg tgtcggtgaa tctgcgccca
    25561 acgcccgcca gcacgctccg ggtgaccgac ccgtcggccc gctcggtgac cgccctcctg
    25621 ctcgccctgc ggcccgcaga ccggatcggc ccacagctca gtgtggcgcc cggtgcgacg
    25681 cccgaagcct ggtcgatcca catcgacgag gaaggctccc gccatgcggt gacccgtcag
    25741 ggcgcccgcc tcgcgatgga cgatgccagc gtgctcagcc ttgccgcacc tgcggacatt
    25801 gcaccggcgc aagccgtcct gcgcgccgct tatcgcaagg cggtggacgc cttcccgccc
    25861 tggcgcgacc tgtcacgctt ccgggcacgg atcgcatacg gattgctcgc attgatggtg
    25921 gcccaggaga tcgcatggca ggtcttcatc cgccggccgg ccaccacgcc cggccagcgt
    25981 cgcggggtgc tgctaggcct gagcgcggga tggatcgcgc tgggtgcgtg gatcttcggg
    26041 atctatctgg cctgagcccc gaccaccgga tgttcaccgt tccggtacgc gcagggaccg
    26101 ggttgccgtc agaatccatc accgccggta caccggacgg caacggccgc cttttgtagg
    26161 gacgcacgat cgaccagacg gctgggcccc cagacgccgg cacgcccggt cccccacgca
    26221 cgcccgcgct gcaccgcaga tcagcggacc ggcgcacccg cttcgctgct ccgccccatc
    26281 gggccgatgt aggacgtggc gaccaccacg ttgtcgatgt acaggctcat gtcccgcggc
    26341 gacggatcaa caccgccgta atagacgttg aaccagacgc tctcgatccg cagctgggga
    26401 acattgcgca gctggatgtc ggtccgctcc accaccagcc ggccgtcgat ccacgcccgc
    26461 aggatgccgt cggacttgcc cggggtgttg agcttcaggt actgctccac gcagtaccag
    26521 cgattgttcg ccagggctgc caggcgcccg aggggccagg gccagaagtc gccggaggga
    26581 tcgcgcatgg cggcgtgata ggcgtaagtg cccatgccgg tgcgcccgga cagcggattg
    26641 ccgcttcggg acaccatgga gaactgcccg cgcagggacc agccatcgtc gccggtactc
    26701 ttgcgcagcc cccaccctgc cttgccgtag gtgccggcga atcccggcat cttgccggcg
    26761 ctctgctccg gcccccagtc ggcggcaaag cgcaggtagt agcggaaata ggcctcttca
    26821 ggctccgaac cggtcttgtc ccggtagtta tagcgcagat ccagcccgag attgccgttg
    26881 cgcgggaccg tcacccgcaa ggcgcggccg ctgaggggca ggaacagccg ctcgggatcc
    26941 gaggtgacgg tggtggatgt gctccgcgaa tcatattccg accacgccga actccagcga
    27001 tccgcctcga aatccgagaa gaacagcact ctgggatcct tggcgatgcc cgcgtccccc
    27061 acataagccg ccgccagacc cggccgcggc agacgatccg gagattccgg cgcccgcacc
    27121 gcaaacacac ccagcgtcgt gtcgttgccg tactgatcgt ccacacgcat tgtcaggacc
    27181 gccttgtcga ggacacgccc ggcaaatttc tcgaccggca gggcgaaggc cagcaggcta
    27241 cgataggtgc cgccggcgcg cagacgcggt tcgaatccga ggcccttttc ggtggagcag
    27301 tcgagatagg catccgacac cggatcgaag gccgtgcgac ggccgtcctt gaattccagg
    27361 cgcaggagcg gtcgggccgt gatgtcggga tgctcccggc tggcaaagac gacgatgttg
    27421 ccgccgggac cgctggtgga catgagcatc gcgccctggg ccggatcggc cttgccggcc
    27481 agccagtcgc gggccagtcc ggtcatgtcg atctccacca gatgcccgcg actgccttcg
    27541 cggatcaccg tcgtggaata ggcccgcgta ccgtaggaac gccggtccgc atcggcccag
    27601 tcacccccct cacggttcca gcgcccacct ccgacgccgt tgaaatagtg gcagctggcg
    27661 ccgtcgatgg cgtcgggcag gctgttttcc ggcgatgcgg cccacgcgcc aagggtcagg
    27721 acacttgccc cgacggccag catccgcccg agcaggcgga tcactcgccg gccctcgtcg
    27781 cggccgcgcc gggcgcagcg gcggcggcac cggaggcgtt cgcacccgtg tttgccggcg
    27841 gggccgcggt gcccggcggg tcttcgggcg cgggctcggg ctggggtcgc cacgcgccga
    27901 tgcgctgcag cttgttgcgc agtccgggca gcggatgtcc cagggcgtag gccttgtggg
    27961 ctgcttcgag cgcctgctgg ggcttgtcca ccatcaacag gcccaggccc aggttgtact
    28021 gatagttggc cgagtccacc tgaagctcgc gggccttggc catctgttcg gcggcttcgg
    28081 ccttcatgcc gcgctggacc agccaaagac cgttgatgag gtacatcagg ccgtcgtcgg
    28141 ggcggaactg aatcgcccgg gaaacccagc aatcgacagt ctccgtggta ccggtgggcg
    28201 tcggggtctt ctgcttgagc cccagcttca ccatggcgtt gagcgcccgg aagtggttgg
    28261 ggaaatagcg cagggtgaat tccagatcgt ggcccacgta ggcgctgctc ttgcccttgg
    28321 tgagggattc cacgccggta tcgaagtggt agttctcaac tcgcaggagc ttgtccggcg
    28381 gcgccacacg gtaatcgtag gggccgaact ccagtgccgg gaccggctgg caggtcccgt
    28441 gctcaccagc cattgccgcg ctggccacca acatgccggc caccacccat cccgcaaccc
    28501 gcccgcggcg gctaaggacg tgcatcgttt ctctggtcac catggaagcc tcgtcaatgt
    28561 gtcgagcctc tgccgcggat caggcgcggg cagacgccag ctggggcgcg tcgacgttca
    28621 gataattgcg caacaggcgc agggagaatt tgcgcagatt gtgatcccag ggcgtgaagc
    28681 gcggcaactc gtacagcccg gattccgaac cgaccgagcc gtgggacgtg gtgaaggctg
    28741 cttcgtagcc gagttcccgg gcgagacgag cgtgcactgc cgtgtaatcc ttgcccggcc
    28801 gaccgttggg ataagcgaag aagcgcggcg gacgtcccag gagttccgcc agacgggccc
    28861 ggtcgcgggc catctggctg cgggcctcgt cctcgtccag caccgccagg atggggtgcg
    28921 aaaccgtatg tccgccaatc tccatgcccg cgtcccgcag gccgcgcaac tgggcgctgc
    28981 tcatcatcag gtcctcgggc aggcggctcc cggtctgggc cacgagccgt gccagcgccg
    29041 ccttccggcc atcgaggtcg aggtatttga gcttcaacag caaggcctcg gccagcacct
    29101 gacgctgcac aacggactgc gtcgcgcaca tccccatgtc gagccaggcc agatccaggg
    29161 tggcaccgcc gtactggcgc agggtctcga tgaccgcgtc gttccacatc aggccgccgt
    29221 cgaggaagcc gctggccacg aagaaggtgg cttccatgcc atgtcggcgc aggatcggac
    29281 aggccactgt gaagttgtct gcgtaaccgt cgtcgaaggt gatcgccacc gaccgtgccg
    29341 gcagggtgcc cgcagccctg cgggacagag cctcggccat gggcaggacg tggaattccc
    29401 gcccgagcat tgtcagcagg cgatcaaagc ggtcggcgtc gatctcgtcc ggattcatcg
    29461 ggtccggttc cggaacgacg cgatgaaaga tcagcacgga caggcgccgg cccttgccgg
    29521 gcaacagcga cgcgaggcct tcggccttca gcgccaggga acggggaagc agacccatga
    29581 attcctctcg ggagacggct caggcgcccc gcaggcgccg ccgcgcccca cgcaggagtt
    29641 ccttgaaacc ggagatttcc gcctcgaact cgatgtcgga aatatcgtcc gcgtcgagaa
    29701 agcggtgcag cgcaagacgg ggcgtcgtcc gttcgttgag gccggcgatg caggtagtgg
    29761 ccgacacgac ccccgctgcc tccagcaccg gaaacgacgc ggcgcgccat tcgccgctgg
    29821 gatagcacag atgccgcgcc gcagccacgc cgcaggcagc cagcgccgcc cggttatcgt
    29881 cgatctcgcg gcgcagttgg gccggatcgg ccgggtagcg gtgccggtgg gtgtgcagct
    29941 cgatggacag cccggccgcc tgcagcgcac gaacctgttc ggcggtgaca atccggaagc
    30001 gctcgtcgag cccggcggat tccggttcga gcacccctgc cgcgcgggcg ttccgcagga
    30061 aggcccggcg ggccggcttg tcgagacgct ggtagcgggc gatcggccca ccctcccccg
    30121 gcggcagcag cgccgcaagc gcgtcggccc gttccgggtg acgacgcagg gcacgccaga
    30181 tcaggtactg ggccaccaga tccgccacgg gacgggcagc cgtctggtag tgggtggtga
    30241 ggtagagcat cgccggaatc cggcgcgcgg ccagggcagg tgccgcatga cgcagcacgc
    30301 tgatgaagcc gtcgtcgatg gtgatgacca cgtcccgcaa gcgtgccgat ccggcgtcca
    30361 gtcgacgcag ggcctcatcc agggtgatga cccggtagcc tcgccgggcc agcaggtcga
    30421 ggcgccgcgc aaatgttgcc ggctgcatga acagctgcgg ccggaaaaga tgctcgtcct
    30481 caaaggcaaa cgcgtggtag cagaggatgc tctggcgccc gcggtagagc cagctggcca
    30541 gggtgaacaa acccagcagg cgggcgagac gatagaaggc cccgcgcacc ttacagcccc
    30601 ccggccggtc gcggcctggg tacggcaggg gcaccgggcg ctgtgacggc gtccggcacc
    30661 ttgcggcaat cccgccagag cagacccacg atggcgacaa ggtaataggg cacgtcgaag
    30721 ttcaccagcc cgaggaaggt cccccccacg gcaaagctga ggagcgagat ctggatgcac
    30781 tgggccagca gcttgagcca ctgttgttcc ggatcgttgc ccacctcctt gatcatccgg
    30841 ccgcaaaagc gccaggaggt gacgcccatg ccgatgtaga agaagaagcc gaggatgccg
    30901 tgctcgccga gggcctggaa gtagatgctg tggggcgaat agggcagcag ccactcggtg
    30961 acggcgtagc gcccccagac ctcccaggtc cagaagtcga accctgcgcc ccatggattg
    31021 tgggccacca tgttcaggat catctgccag gtgtagaggc ggctctgggc ggagtggtcc
    31081 tcgtgggacg agatcgtgtt catccggttc gaccaggtgt ccggcatgaa ggccgccaaa
    31141 ccgatcccga tgatggcgaa cagggacacc gccagcaccg gtcgcttgct gcgcgcaccg
    31201 aggatcagcg ccatcgcgct gatggccagc agcgcgccgc gggactggga gccgagcacc
    31261 gagaaggcca ccaggatcag cgagatgctg agcaccgtcc gccaccaggc ccgctcggcg
    31321 gtctgacgca tgaagtacat gaggggcatg atggtcacga gggccagggc cagctcgttg
    31381 ttgccctcga aaaagctccc agtcggcccc cacacccgct cgccaccgcc ggaacggatc
    31441 gtaaacagcc cccccttgat cccgtagaag ccgatggaca gggtaatcac cttgatcaga
    31501 tcgaggatct gcttcttgcc cccgatgagc gcgatggtca cccacagcat cacgtgcacc
    31561 ttggtcacct tgacccacat gtcgaaggcg tcgcccacgt tgttcagggc gatgatggtc
    31621 gtgaagcaca tcaccgcgta gaagtaggcc aggtagcgga tcggcgtgat ccacgggatg
    31681 cggtgccgct ccttggcggc aaacagtgcg atgagcgtgg cgccgccgac cagctgcgcg
    31741 aacggcaggg aataggcgaa cccgaaggtc aggcggtgcg gcaccatcat gctcacccat
    31801 gcccaggcga gcggccccag ggacgggcgg aagaagatgc tcggcaccag gacgagcatg
    31861 atcagggtca gggctacgtc acgcacgcgg ggctcccgcc ggcgtccagg ctgcgaccgg
    31921 catcatgctg gaggggaaaa acatcgtgtc gcccatctca gcgtttgacg tggctcacga
    31981 catagcgggt cttgcccgat ttctccggcg ggatctcgct gaccggcgtg atgtccacct
    32041 gcacgcccgc gcccaggcgg gcggcgtagt cgtggcggat gcgctggcgc tcggcctcac
    32101 cgaaccggtc gtccgccacc accagcacct cggtacggcc gatgtcgtgc tggacgatct
    32161 tgaactgctg tacgccaggc agatcccgga cggtatagat gagcgcgagg gcgtgcatca
    32221 ccgtgccgtc cgcgcccacc acgaagtcgg tggtgcgccc ctgcacctcg gccaggatgg
    32281 gcaggttcac accgcatccg caggtttccg tgctgagcac gccgatgtcg ccggtgcggt
    32341 agcgcacgaa ggggaaatcc cgcgtggcgg tgtgggtcac gacgatctcg ccgggcgcgc
    32401 ccggcgccac cggccggcct tccgggtcga tggtctcgac gacgatgtcc tcggccgaca
    32461 ggtgcaggtg gccgtggggg cactggtggg cgatgaagcc cgcgtcccgt gccccgtagc
    32521 cattggccac cgggcagccg aagatttcgg tgatggactg cttctggtgt gcgtagagca
    32581 tctcggaggt gacgaaggcg acgcggatgc ccaggtcgtc catcgggatc ttccacaggc
    32641 gtgcccgctc ggccagatgg gcgagggccg aaggatagcc gaagagcatg cgcgggcgct
    32701 tggcgcggat catcgccagc atgtcccgca ccttggcgtc ggacatctcg aaggccggca
    32761 gcagccaggt gcgcagcagg ctgtcgcgga cggccttgac ccggtcctgg gcacccagtt
    32821 cgatgggcga gccccacagc acgatctccg gatcgccgat atccacgtcc caccaccgcg
    32881 tcgcccgcca cttggcggcc acatcatggc tcttgcgcgc cttgccgatg tagaagatca
    32941 gcggttcacc gctggaaccg ccggtgttgt agcggctcag gggaccggcg tcgtcggcct
    33001 tcagttcgtc cacgtgagcg cggatgacgg ccttggtcat gaagggcagc ttgcgcagat
    33061 ccgccgcacc ggtcacgccg gccggatcga aaccgtgttc ccggaacagc gtgtggtagt
    33121 acggcacatg acggccgatg tcttccagga aggccttcag gcggcgggcc tggatcgcct
    33181 cgatctcggc ccgcggcaga cgctgggtga tttccagatc tttgcggcgg gcaacggagt
    33241 cgtgcttctt gagtcgttcg tgcaggggaa agagcacccc ggagcacacg cgcgtataga
    33301 gtcccatggt cctgtcctgc tagcggttgc gcgcgagccg gcgatagacg gccagccact
    33361 tctggcgcac caccggccac gcatactgtt tgacttcgtc ccgcgccgcg gcagcgagcc
    33421 gttcggccag aggccgatcc cgcaggatgc gcagcatcgc ctcggccatg cgcgccggat
    33481 cgcgggccgg caccagcagc gcggtacggc cgtcctcggc aatgaagggc acgccgccca
    33541 cgtcggtgga caccaccggc accccgctcg cgaaggcttc aaggatcgag atgggcatgt
    33601 tgtccaccgt gctcgggttg agcatcacgt cggctgcggc gtagagggcc gggatctggg
    33661 cattgtcgat gcgcccggca aaggtgaccg cctcggcgat gcccaggtcg gcggccatgg
    33721 cctgcaggcg ggtcagctcc gggccgctgc cggccaccgt gaggcgtgcc gccggcagcg
    33781 ctgcacgcac tgcggcgaaa gccgccagca cgctggggat gtcgtagatc ggctccaggt
    33841 tgcgcgtcac caccacgtgg ggcgcgtcgt ctgccgtgcg cgtgcggggc acgccgaagc
    33901 gttccaggtt gatgatgttg ggcacgatct ccgacgcaaa gccgaagcgc cggaacaccc
    33961 cgtcaagaaa ggccgacggg accaccagcg catccgcggc cgcgagggtg cgatgcacat
    34021 ggcgcggcgc ggcggcaaag aagggctccg cctcgccccc ccggtaattg acgatgaccg
    34081 gagccccccg caggcgggca atccagagcg ccggcgcggc aaacaggtgc caggcccagc
    34141 cggaattggc cagcacatgc atcacctggc agcgcccgct cgcccgccac agggcccaca
    34201 gataaggcag cagccggaag aaggcgcgca acaccggaat gcggccagcc cactcgggct
    34261 gatacggcgc attggtacgg accagctcca cctccgcgcc ttcggcgcgc agcaggccga
    34321 gcagttgctg gcactggttg gccatgccgc cggagggcgg cggaaggggt ccgacgacgg
    34381 ccagacgcag gccggaatag gtgggttgtt ccatcacagg gggttctcgc gccggcctga
    34441 acggcgccat gccagaatct gaacgagcat caggaaggcc agcacggcct cgaccgcagc
    34501 gatcaccctg atctgggtcg ccaggcgggt catccgggcc accagggcct ggttgctggg
    34561 tgtctcgacg atgcggacgt ggggatgcac gccagcgacg atggcgccca gctgctccag
    34621 ggagcgggac acgtgcccga gggccgcgac caccggtgcc cgctcgtaat cgaacaccgc
    34681 gttgccccac cacaggtacc actggtccat gccgtgacgg tagtaggcca gcagcttgtc
    34741 ctcgtacttc atgccagcgc gccccggacc ggtcttgacg atccttaccg ccgggccctc
    34801 gccatggagg aatgcccgga attcggcgac gctcaggatc tcgcccgaat ccgcatagac
    34861 cggatgaaaa ttgttgaaga cgtccaggaa catccagcgc tggtgctgcc ggtcgaagac
    34921 ctccaccacc gcatggccgt gcccgccgaa gccgtcaaag gaaaaccccc actcccgcgc
    34981 gaacagaccg gcggcccggg ccaacgccag atacaccgcg gtgtagtcgg cacagtagcc
    35041 agcgcctttc cggatggcgt ggtaggtcgt ctgcaggtcg gactggatgg ggccctcctg
    35101 gtcggcgtgc tggctcaggt gggccgacag ggccagcgcc ttggcgcggt cgtccggcaa
    35161 ggccgcgatg cccagattgc gcaccacgtc ggcgaacagg ggctcgaccg ggccggcatc
    35221 ctgcaggaaa ccggccggca cctgcgcggg cgtccacgtg aagtcggcgg gcgaggacgg
    35281 ctccacgagc agtgcattac gcaggcgcac cgcctcggtg gacgggatcg cctgcacccc
    35341 gagaaaggcc gccaggatcg ccatacacac cacggtggcg agcagcagcc ggggcatcgc
    35401 ccggccggcc acccggcgcc ggagagctgt caccagcaga gtccctcgac gtccgcctgg
    35461 atgtccgcca cattgggcag attgaccagg tcgatgatct tctggccggg acgggccaga
    35521 cgggccaggt ccgcgaaaac ttccttgctc gacaggccta ccaccagcac ctcggcctcg
    35581 gccaccacat cggcgagctc cggcttgagg agttcgccga tgtggggcac gtgacgctcg
    35641 atgaagctct tgttggcgcc cagcaggcgg gacagatgca cttccggatc gtagatggag
    35701 agctgcacgc ccttgcccag cagctgttcg gcgagggaca ccagggggct ttcccgcaga
    35761 tcgtcggtgc cggtcttgaa ggacagtccg acgaagccga ccttgcgctt gccggtttcg
    35821 agcaccttat tcaccgccac atccacgtga ttgcggttgg actgcatgat gcccgccagc
    35881 atggggatct ccacgtcgtg gcgcttggcc aggtaggtgg tggcgcgcag gtccttgggc
    35941 aggcaggagc cgccgaaggc atagccgggc ttcaggtacg cgcgggagat gttgagctgg
    36001 gtgtcctggc agaccaggtc catgacctcg aagggatcga cgccgagggc ggcgcacagg
    36061 cgcgcggtct cgttggcgaa ggtgatcttg agggcgtgga agttgttgca gcagtacttc
    36121 atcatttccg cggaacgcac cgaggtcttg aggaagttgc agggcagatg accgaagagc
    36181 gcctttagac gctccgccgg gtagtcgtga ttggcgccca ccaccgtaaa gggcggcttg
    36241 tcgtagtcgc ggatcgacga gccttcccgc aggaattcgg gctggaagca cagatggaag
    36301 ccctctccat ccttcttgcc agagccgttc tcgatgatcg gacgcagcac gtcttccacg
    36361 gtgccaggca ccagagtcga acggaacacc accacgtggg gcgcggcctt ttcgcggatc
    36421 gccacgccca tgctctcggc cagacgcagc acggcgccct ggtcctggct gccgttggcg
    36481 gccgacggcg tgcccacgca gaccagcgag atgtcgctgt tgagcacggc atgcacggcg
    36541 tcggtggtca cagtgacgcg accggcggcg accacgtcct tcatcaggtc caccatgccc
    36601 tcctccacca ccggggtctt gccctggcgg atcaggtcga gcttggtggc gtcgatgtcg
    36661 acgccgatca cgtcgtgccc gtcacgggcc aggcaggcga gcgacaccgc tccgacgtaa
    36721 cccaggccga aaatgctgat cttcatgtgc gcttttcctt gctttcctga aattgagtga
    36781 tgcagcgtcc gatgatccgg tcaagccgcg ccatggacgc aggccaggcg tggtggctga
    36841 gcatccgcgc ccggccggcc tcggccagac ggcggcgctc gaccgggtct gacgtgatgc
    36901 gagcaatagc cgcagcataa gcttccgggg tatcggcgac gaggaaatgc ttctcggctt
    36961 ccgcatcgac gccgcccgcc gcgatggaac tggtgacgac cggcacgccc atggccatcg
    37021 cctcgagaat cttgttctgg gtcccgcggg caatcgcgag gggcgccacc atcagcgccg
    37081 agctgcgcac gtaggggcgc acgtcgggca ccgagccggt gacggtcacc ccgtccagtt
    37141 cgcccagctt gcgcatggcc ggggaaggat ccgcaccgac gatgagcagc ttcatctggg
    37201 gccgcgccgc tttgacccgc ggccagatgt cctggcagaa acgggtcatg cattcctggt
    37261 tggggtaata gtccatccgc ccgatgaaac tgatggtgtc gggatcgtag accgtgtcgt
    37321 cagggcagaa gaagcccgcg tccacgccgt tcgggaacca gtcggtggcg aggccggtgc
    37381 cgtagctgtc aagggtttcc cactcggccc gggtggtggc cgtgcccaga tcgaacttgc
    37441 cggcgaggcg cttttccgcg gccagcatct tgaggccttc gaggcggtag ccgagggaca
    37501 gggggaaggg cttgtagtgg gcgtactcaa gccacttctg ggaatccatg tcgccgtagt
    37561 cgatgatctt gggcacgtcg gtcacgtgct ccacgtactg ggcgacagac gagcagtgga
    37621 cgaagatcag gtcccacttc tggctggcga ggaggcggtc gatcttggcc ttcaggtcgg
    37681 gcgaatagaa gaagcccatg gatgagggcg tgctcaccgg cagccgcgcc accatgcgca
    37741 gggcctgcac cgggttgctc acgagaccca tctcgaaggc cttgcaatga ggggcgatgc
    37801 cccgcccctc ctccgcctcc tggggcgaac gggcgaggga gcagacggtg accgcatgcc
    37861 cctgcgccgt gaggtggcgg atcatgttga agggccggat cttgccgccc cgcttgggcg
    37921 ggaagggaaa acgatggcag acgtacagaa tgttcatcgg gtgtagctct tcagctcggc
    37981 cgccacgtcg tcggccgacc gcccgtcgag gtagatccgg ctccagattt caagattcat
    38041 gagggacagc aaaccgtccg tgccgtcgct gcggttggat tcgtggtcgg ccatcaggcc
    38101 ggcgatgacg tcatggcgaa acaggccacg gcggcgcacg gattccggcg acaggagctg
    38161 acgcagcacc ggcgccagat cgcgcttgag ccaggcgccc atgggcgtgc cgaagccgcg
    38221 cttcttgcgg tcgaggatgt cggccggcag caagtccgcc agggcatcct tcatcaggtg
    38281 cttgagacgt ccgccgcgga tcttcaccgc ggcgggaatc gccgcggcga gttcaacgag
    38341 ttcgtgatcc agcagtggaa cgcggcattc cagggacacg gccatgctca tcttgtcggt
    38401 gagcaacagc agatcgtccg gcagctgggt ttcggcgtcc accgcgaaca tgcggttgag
    38461 gtcgtcggcc tggccggcgt cggagaaggc ctgggccagc ggatcgggcg cctgggcgcc
    38521 tgcatccagc aacatggcgg agacggtttc ccgcgccagc acctgcaggt agctgcgata
    38581 ccggtcgtcc ggcgccatgt ttgccgaggc gatgaagccc ttggccaggc gcagggtgtt
    38641 gagcaggccg gaatgccggt cgctgggcag gcgattggca actgccgtgg ccgcgctgcg
    38701 cacgaagccc ggcaggcgct ggaaacgctc ggcgtaatgg ccgcccaggt agcgccgata
    38761 gccgccgaag agctcgtcgc cgccgacgcc ggagaggatc accttcacgt cctggcgggc
    38821 gaattcggag accagaaagg tggtgatgaa ggccgtgtcc gccaggggct cgtccatgtg
    38881 ccagagcagc ttgggcagca gcgacaccac gtccggcttg acgacgattt cgtggtgctc
    38941 ggtgccgaag agctgggaga cctggcgggc ataaggcagc tcgttgtaga gcgcctctgc
    39001 ctcgccgccc tcgaagccga tggcgtaggt gcggatcggc cggtcggagt ggcgcgccat
    39061 gtaacccacc acggcgctgg agtcgacgcc gccggacagg aaggcgccga tgggcacgtc
    39121 actgaccatc tgcatgcgca cggactcttc gaggcgcacc cgcacccgct ccagccagtc
    39181 ggcctcggat accgtgcgct ctacgacgct gggcaggcgc cagtagcgcc attcgcggac
    39241 ctcgttgttc tcgatggaca gcagggtcgc cgggggcagc ttgcggatgc ccttgaacat
    39301 ggagccgggc gcagccgcgt agcccaggtg caggtagctc gcgagctggc tgcggtcgag
    39361 ctccggcgag acgccgggca gggccagcag ggccttggcc tcggtggcaa aggccagacg
    39421 ccgggcgtcc tgcatcacgt agaggggttt gacgccgatg cggtcgcggc cgatcagaag
    39481 gcgcttgcgc cgccggtccc acagggcgaa gtcgaacatg ccattgaggc gatggacgaa
    39541 atcgtcaccg aaggcgtcgt aggcgtggag cacgacctca ctgtcggagc cggtcttgaa
    39601 gtggaagccc aggccctgca gttcggcgcg cagctcgcgg aagttgtaga tctcgccgtt
    39661 gcagacgatc cacaggctgt cgtcggcgtt ggagatgggc tggtggccac cggccaggtc
    39721 gatgatggac agccggcgca tggcgatccc gcagggaccg tcgatgtact ggccttcgtc
    39781 gtcgggcccg cgatgctggg tccggtcgcc catggcggtg agctgggcgg gttcgacccg
    39841 ctgaccgtca aatcggaaga atccgtgaat gccgcacacg ttgcaacctc agatggaaag
    39901 ggtacggccg cgggccgcca gggcccgccg gtagacgtcg cgataacggg ccacgctggt
    39961 ggcccaggtg cgttcgacct cgacgaaact ccgggcctgc tggcgcacct ggggccagcg
    40021 ctcgcgatgg gtcagcacgt cgtcgatggc tgccgccagg gcgtccacgt cgccggcgcg
    40081 aaacaggaag ccagtctcgc cgtggcggat gagctccttg tgtccgccca cgtcggaggc
    40141 cacgaacatg cgtccctggg ccatggcctc cagcggcttg aggggcgtca ccagctcggt
    40201 caggcggatg ggcaggcgcg gataggcgag cacgtcgatc agttcgtaat agcgctgcac
    40261 gaccgcatgg ggcacgcggc cggaaaagat gacccggtcg gacaagccca gctcggcgac
    40321 cagggccttg aggttcgcgt cctgcggtcc gccgcccacc agcagcacgc gcaggtccgg
    40381 gtagcgatcc accagctggc gcgccgcgcg gatgagcagg tcgagccctt cgtagccata
    40441 gaaggagccg gcgaagccca gcaccgtctt gccgtcgagg cccaggctgc ggcgcagttc
    40501 cgggtccgct tcggcgccga aggtgaaggc ggatacgtct acggcgttgg gaatcaccgt
    40561 gatgcgctcg tccggcacgc cgcgtacggc gatgtcgccg cgcaggcctt cgcagatggt
    40621 ggtcacctga tccgcatggc gcagggcgaa gctttcaaga ccgcgggaca cccggtagcg
    40681 caggctccct tcggcggtgg tgccgtgatc cacggcggcg tcttcccagg aggcgcgcat
    40741 ctcgtaaacg accgggatgc ggtgcttgcg cccgacccac agggccggca gcgcgttgag
    40801 tacgggcgaa tggggatgga ggatgtcggg tcgcagcgtc tgcaccagat cgtcaagccg
    40861 cgcggcggtg aggcgcatct gcgcgaccag gccttcctgc ccggtgttgg cggttcggtg
    40921 gaaggtccag ccgtcggcct cttcggcacc gccggaggag acgccctgct tgggcgtggt
    40981 gacgtgcaag gtttcccagc cgagcttgcg ctgctcccgc aggatggaca ccgtgcggaa
    41041 ggcgtagccg ctgtgcagcg ggagggaatg gtcaaggacg tggaggacgc gcaggctcat
    41101 gccgacctca cgagcggcgc gggctgggcc gtggtctgac agttgcgcag gaaggcctcg
    41161 aacatcaggg tggtccagat ggcggagctg tagtcacgag cgccggattg gtgggcatcc
    41221 accagatggc gcaggtaggc ttcgttgaag agcccggtgg cgcgcagggt ctcgccgagc
    41281 agcgcctcgc gcacccgctc cttgaggggg ccacggaacc agcgcgacag gggcaccgcg
    41341 aagcccatct tcgggcggta cagcacgtcg cggggcagca tcggctccat ggacttcttg
    41401 aacacgtact tgccttcctg ccccttgatc ttgaggctgc tgggcaggga cgcgagccat
    41461 tccacgaggg gatggtccat cagcggttcg cgcacttcca gggaatgcgc catggaggcc
    41521 cggtccacct tggtgttgat gtcgcccacc agataggtct tgatgtccag gtactggatc
    41581 agcgccaggg ggtcatcggt ctgggcattg gcggcgtggc ggcggaacac ctccagggcg
    41641 ttgtaaccgc ccagttcctg acggaaacgc tgggagaaga gctgccgacg catgtcgtcc
    41701 cgcaggatcg acacggaatg gaaataggcc tggaccgaat cccgcgcaag cgcctggaag
    41761 gtggtcttgg cgcggaacac ccgcggcgcc cagtcggcct tgggatacac ggagccgagg
    41821 aagccgaaga gcggtcggcg caggccgtag ggcagcccgc cacgcatctt ttcttccatc
    41881 atgtggaagc ggtagcggcg atagccgccc atgttctcgt caccaccgtc gcccgacagg
    41941 gccacggtga cgtgcttgcg ggcgagctgg cagacccggt aggtgggaat cgcggagctg
    42001 tcggcgtagg gctcgtcgta cagcgcggcg agggtgtcga tgaggtcgaa gtcgtcggac
    42061 tggacggtct cgacgcgatg attggtcttg tagcggtccg ccaccatctg ggcgaattcg
    42121 gattcgttga aggccggatc gtcaaaggcg atggagcagg tattcaccgg ctcgtccgac
    42181 agcccggcca tggtcgccac caccgcgctg gaatccaccc cgccggacag gaaggcgccc
    42241 aggggcacct cggcgatcat gcgcaggcgc acggactcct tcaggcgctc cttcagctcc
    42301 gccgtggcgt cggcgaggtt caggttgtgg tcgagggtga aacgcacgtc ccagtagggc
    42361 ttgggctggg gtattggctc gccgcggcgg atcgtcaggg tgaagccggg atcgagcttg
    42421 cgcgcgcctt tgaagatcgt gcgcggctca gccacataac ccagggcgaa gtattcctcc
    42481 accgccagcg ggtccatggt ccggtccagg tccgggtgct gcatcagcac cttgagttcg
    42541 gagccgaaga ccagcgtgcc atccctcagg atggcgtaat acatgggctt cacccccatg
    42601 cggtcgcggg ccatgaagaa ggtctggcgg ttgcggtccc acagggcaaa cgcaaacatg
    42661 ccgcggaaac gctgcacgca ggcctcaccc cacgattccc acgcatggac gatgacttcc
    42721 gtatcgctgc gggtgtggaa gcggtgaccg agggcggtga gttcgggaat cagttcctga
    42781 tagttgtaga tctcgccgtt gaacaccacc accaccgagc cgtcctcatt gaagaggggc
    42841 tgctgacccg tggacaggtc gatgatggac agacgccggt gggcgaaggc cacccccggc
    42901 tcgaagtgat agccgctttc atccggtccg cgatgggact ggatgtcgtt catccgctgc
    42961 atcagctcgc gcggatagtc ctgctggccc cgcgtgtcga atattccggc aatgccgcac
    43021 atagtcccta ccttaaattc cgcgttgccg cttagctcgc gctgtcagcg ccccttggcc
    43081 gcgcacaggg ccagatagag atcggcatac cgcttcacca tgccgtccag gctgaaattc
    43141 gcctccacgc gcagccttcc cgcgtgtcct tccgcccggc cccgcagcgg atcccgcacg
    43201 tactccagca gcttatcggc aagacgctgg ctgtcctgag ggggaaccag cgcgccgctc
    43261 tccccgtcgg cgaccagttc gccgttgccg cccacgtccg tggccacgac cggcaagccg
    43321 gccgccatgg cctccaggat cgtgttcgag atgccctcgg tctgggaggg cagcacgaac
    43381 agatcgaaac cactcatcat ccggggtacg tcagaacgct ccccggccat ccacacccgg
    43441 ccattcagcc cgagcgtccg gatctcatcc tcgatccgcc cacgcagcgg gccgtcgcca
    43501 gccagcacca gacgcaccga ttcgctgtgc gtcgcatcca attgacgcat ccgggcaaag
    43561 gctcgcacca gattgagctg atccttgacc gcctgcaggc gccccaccgt gccgatcacg
    43621 aaatacgccg gatcgttgaa gggcgatccc tcgagcgccg cacgacgcgc gcctcgcggc
    43681 gtgaatcggg cgacgtcgac gccgttgcag atccgctcgg cccgtctgtc tggaatgccg
    43741 accgcacgca ccagatatga caggatctgc ccggacagcg caatgtaatg agtcacgaag
    43801 ggtctgtaga gccggcgcat ccagcggaac ttggcgcgcg tgccatgtgg atccgagaca
    43861 tcccagccgt gctctccatg aatgcggacg ggaacacccg ccgcccaggc cggcaccgtc
    43921 gcctccagcg ctgccagatt gcgggtatgc acgacatccg gacgatacgc gcggaacagc
    43981 ctgaacaatg ccggatagat cttgaccccc tgccctggcg gtttgtgcag tgcgacgaat
    44041 tctacatccg gcacctgcac gcgggcacag aaaagcggtt cacattgtgt caatgccacg
    44101 acgatatgcc gaaagcgtgt ccgatccatc cggttgatca gattgacgac gccattttct
    44161 agcccgccga cggcaaagct atacaccacg tgcatgacga gcaatcgccc gtcagggtga
    44221 agcgcgctca tttcacgcgg tccagcgact ggagctgctg gaacagcggc ccctgcaggg
    44281 acgcggcagc atcctccagc gcctgttcgg cggaacgctc gtcgggcgcc cgcgaccaca
    44341 tcgccacata ggccgacgtg tcagccttgc cggtcaaccg ggccagcgtc aagaggccct
    44401 tggcgacgta atcgctggtg accagtcgac cgtcgatcca gtacgcgctc cagaccacga
    44461 tgcgcgtccc ctctccgctg aggaccgaac gcttccaggc gccgaagggc gtctccggac
    44521 cggccccctc gtcgagcatc gcccaatggt gatcctcgac cgacagcagc acattgcctg
    44581 cgcgaaccaa ttcacggccc tgggactgat tcgcgtagac cgccacatag gcattcaccg
    44641 tgcgccgccc gtcggaatag ccttcctcca tcagcccgcg atggccgctg aagcgtggct
    44701 ggaatgcgtg ccccgtttcg acaccacccg ctcgccatgc gccaccggcc gccggcaacg
    44761 ctatcgggat ggtgacatcc cgatcagtcg gcgccgcgag caggacggcg accaccggcc
    44821 acaccgcggt cagcagggcg aacggcagca cgccccgcac cagcgccgcc gtgtcgattg
    44881 atgcggccgg cgcagggacc gccgcagccg ccggcagctc atccagatcc tcccgccaga
    44941 acgagcccac ccagaacatg agcaggatca ccagcccgaa gaacacccag ccatagatca
    45001 ggtgatcaac accggtggcg agctggttat tgctgaagta gcccagcatc acgatcatga
    45061 aggccctcag ccagttggcg accaccggca tcacgatggc caccgccgtg aagatcagcc
    45121 gtcgccgcag actacggaaa ctgagatagg cgtagagggt cccgaccatc accgacgcaa
    45181 tcagataacg aaagccgctg caggcctcaa tcaccgacca acggccgttg ggcaggacga
    45241 aatgcagccc ttcctggtac acgggcacgc ccatgagacg cacggccgac acggtaaaca
    45301 aagccgtgta cttcatcatg acaggtaaca ggaattcacc tgcggggatg ccgaacagca
    45361 gaaacagcag cggaaatttc agggcccccg cggcttcgcg gccgagcacg ccgaagagcg
    45421 tcagcaccag catcgtgaca aggcagaaat gcgagaccga tgccacgctg cccaactgcc
    45481 cgagcagcca tccaaagccg gccaccgcga ccggcaacag ccagatcggc cacgcgcgca
    45541 gcggacgggt cgccagcacc tcgcgcttgc gccataccag ccatgcaaag atgggaatca
    45601 ccatgaggcc gtgggcatag gattccgaac gccaccatac cccggcgatg tcagccgccg
    45661 tggtgaagta ggcgccaatg atccacagaa acacgccggc gatggccacc ggtaccgctc
    45721 gtcgtgaatt catgccttgt ccgcctcgca cgcggcacgc ggcatggacc gccgccgcgc
    45781 ctcaatgaac gtcgtcacca tgtttaaccc agattccgga cgatcatggg gcccaggcga
    45841 ttggccaccc ccaccggaag gcgcttccac agggcaatga aggcgcggta cttggggttc
    45901 atgggattgt tctggggaat ctcgtcccgc ttgaaaagcc ggtattcgta cgaaaggggc
    45961 atgggctcga agccccagtt cttcttgaaa ctgtacgggc ccgtgccgat cttgctgcgg
    46021 ccatagtcga agaggcggca gccccgggcg caagcccgtc gcatcagttc ccagtatttg
    46081 aagtcgttgg cggccagatc ccgcgcctcc atgtcgtcac cggcgtaata gggcaggatc
    46141 tcttcacgga agtagaagct cagcacggag gacaggagct tcccgtcggc cgtggtcacc
    46201 gtcatgactt cgcaatcctt gccgaacact tccagcaggg tctcgaagaa acgcttgggc
    46261 agcgccggcg taccgtggcg caggacattg tccgaataca gggcgaagaa acggtcggcg
    46321 ttgggatcga cgtgactcac gagtccgttc ttgatgccct tgcgaaccat ggcgcgctgc
    46381 ttgcgcggaa tggccagcat gttcgcctcc tcgtcgggca ggatctcctt gcggaaggtg
    46441 acgtacagat cctgccgcgg ccagtcttcg tgacgggccc gcaggttgcg atattccagg
    46501 tgctggacgc cggcctgtcg ggaaatggtc tccgcctcgg cctccagcgc agccaccgcg
    46561 tcggcgtctg agcctgccac gccgccatac actgcaaacg gcagcgaggt catggaatgc
    46621 ccgaagaggc ggctgttcac ctcaaccagc ggcagcaccg cgacgatatg cccatcccgt
    46681 tcggccagca ggtaatggca acgatggcgg aagacccgct cgatgatgtc gcgccattcg
    46741 atgcggtgaa agaaggtggc gtcggggcag tcgaagacga atgcgttcca gcgcgtggaa
    46801 tcgcccgcct ggaagcggcg tacggaaatc ggtgaagcca tgtatcggaa cctaagctga
    46861 cggcgtgtcg ccggaaagga acacctcgtc catccgtccc cacgcaaaat cattgagcag
    46921 acgggtgaag cgggcctcgg tgcgctccag attgacatag tggcggaagc gggtcttggc
    46981 actcgcctca aggaccaccg gttgcccggg atcgagttcc cagggatgga agtagaagat
    47041 cgccggctta cggtcacctg cattcacctg gcgaatgccc cacgccgaca cgccataagg
    47101 cagcaggcgg aaatagccgc cgccgcccac cggccagttc cgcccgaaac gccggaccgt
    47161 ggtgatggga atctcgacga gactgttgcc gaccgggtac gtgaaacgcg gcgcatcggg
    47221 cacaccgtag tggtcatggc gcaccggata cacacttgaa ctgtagacgt aacccgcggc
    47281 ctggatgcag tcgtgggccc acgggttgct cagtccgacc gaaaagcttg gcgcacggta
    47341 gccacgcact tcgaggccgg tgatgtcttc gaggatcgcc ttggccaacc ggatgtcgca
    47401 caggaaggct tcaggcgact gatcgctggc ccgctcgtga ccataaccat gactggcgat
    47461 ctcgtgaccg gcttccgcaa tgcgccggac cagcgccggg tagcgctcgg caatccagcc
    47521 cagggtgaaa aacgtgccct tggcaccggc agcatccatc agcgcaagga tgcgatccac
    47581 gttggcttcg acgcgacagg gcgtgccatc ccagcccgag cgtggaaaat gcggcgcgag
    47641 cgcggaaacc tggaagtagt cttccacatc gcaggtcagt gcattgacca gtgagcgggc
    47701 gtcgtcgaca tgcggattca tcgggattcc gtttccttgc ctgccgtctg ggacggcttc
    47761 tgtacggcct ggagaagctg tcccagcgca ttcagcgtgg tggacagggt ccgctccagc
    47821 aaggccagac gctcttcaaa gcgcgcggtg tcgaagcctt cgcctgcctt cgccagcgtc
    47881 cgcagggcct ccggctccgc cgccaggcgc gccagatcga cccccgcaag ccccgcggac
    47941 agcgccgctg cgggcgtatc cgcctgcatg gcgggcggcg ccacctgctc ttcacgcatc
    48001 tcggccacca cctgctccac gatggcagcg gagattcggc gctgttccgt caggaatgcg
    48061 gccagcagga cgcgatcgca gactgcgttg atgcggcggg gaatgccctg ggaacttgca
    48121 tggatcgcgg ccagcgcgtc cgcgtcgaaa gacggcacac cgcgccagcc gacgcaattg
    48181 aggcggtgtt cgatataggc ctgggtctcg gttgcatcca gcgggccaag gtggaaggac
    48241 gcgatcacgc gctggcgcag ctgcagcatc tgcggacttt gcatgatggc gcggaattcc
    48301 ggctggccaa cgaggaagct ctgcagcagc gcatggtcct cgagctggaa gttggagagc
    48361 atgcgcagct cctcgaccgc ccggggagtc agattctggg cctcatcgac caccagcagc
    48421 gcacgcttgc cggctgcagc caccgagacc agataggttt caatggccac cagcagatcg
    48481 ctcttgctca tctgccggct cggaatgccg aaggcggcgg ccaccagacg gaggatgtcg
    48541 tccgcgtcga tctgtgtgct gaccagattg gccgccacga tcttggacgg atcgagcttt
    48601 tcaagcaggc tgcgcacgag cagcgttttg cccgccccta cctcgccggt gatcacgatg
    48661 aagccttcgt tctggtgcag tccgtactcc agataggcca tcgcccgccg gtgaccgcgg
    48721 ctgccataga agaagctggg gtccggattg agctggaacg gcttgccgcg caagccaaaa
    48781 tgggattcgt acatgagcgg tcagaagagg atgatcacgc tccccagcag cgcgttttcg
    48841 gtgtattcgg tggtgccggt ggcccggctg tggcggtagg tcagagagcc gctggaacga
    48901 cgggtgaagg acgtggagag gcccgccgtc gctcgccggg tcttgttggt gaaggacggc
    48961 gtcgtggcac tgccgtcagc ctgcgtgcgg atcagcgtca tgtcggccgt cgcggatgta
    49021 agcggcgtca ggcgatgatt cagcatcaga ctgccgctgc tgtcggtcac acgggtggcg
    49081 ttgatgaatt ccccgccgag cgccgcagtc tggctgacca gcgagctgcg ctccgagcgg
    49141 tagccgctga acatgaacgt cgtccgtcgc agccccacgt aggaagcccc gaactgcagg
    49201 cggcgatcaa ggaagacccc gttgttgagc accgactggc tgatgccgat ctgctgatcg
    49261 gtggacaccc cctgattggt caggtaagtc cggaccgcct gatcgcgggc aaccggatcc
    49321 ggatagcgcg atgcgagcag gcccatgagg agatcgtagg ccgtcgtgag ggacgttgcg
    49381 ctgagcgcct ggctggtgct ggtgacgtcc ttggagaact ttgcattgaa gacgaacgga
    49441 cccgtggagt agtccccgct catctggtat ccgggcccga agaagcgatc ttcccaggtg
    49501 gcggacacgc ggccgaacgc agacggcttc cagtcgaaac cggcccccac gatggtggat
    49561 tgctgggtat tgctgctgtt gtagttgttg ccttcaccac cgccaatcac ccgcagctgc
    49621 agctgcggcg tcacccggct catcagcgtg agccgcgccg actgaagatg cagatcacgg
    49681 cgtccggtct gggaaacctg attgtcgctg aacgccagcg accagccgga ctgcccgaac
    49741 gcggtttcat ccacgaggct gcccgaccac atctgctgtt gcatgcgctg gaaggacccg
    49801 gagctggcgg aggacgagtt gatgtcgtag cgcagttccg ccacacccga attgtggaaa
    49861 tgaccgcgca ggtacgggct gataccgacg ttccggacat tggtctggtt ggcgcggccc
    49921 gtcacctgat cgcccggacg cacgccgagc accgaggcga cttcacggga ttccgatccc
    49981 cgcacgtcca catacgcaaa tttttcgaca gcctcgacac ttcctgtccc gttgaacgtc
    50041 aggaaactgg tgttgaggtt cgaattgttg gcgtagaaga gactcgcgac gctggcgcgc
    50101 aggttcccct gggcgcgacc tccccgactg ttcagctcca tggacggaat gatgccggtg
    50161 atgtaatcgg acttggacga cgcattgccc cccgtcccgc tcccgcggct caggtcgacg
    50221 ttgtcggtga tcgtctcggt caggcgcagc gagggggaga acgtcacctg atgatcctga
    50281 gccagcacgg acatgctcgt cagggcaagc ggaatggtca ggacgctcat ccggacgacg
    50341 gtccgggaca gagctcgacc gtgaacgggg cttcggcgct tcacgcattc tccttctggg
    50401 attgcccgta gccgtagcca tagccgtaac cgtatccgta gccataaccg tagccgtaag
    50461 caccggagcc cgcctggagc gccttgttga gcagcatcag cttcaccggg caggcctcga
    50521 tcgtggagat cgcctgcttg accgcggcct gcgtggtctt gttggactcc accaccatca
    50581 cgatctggcc catgtgggtc gccagcacgc gggattccgt cgtcaccagc agcggcggcg
    50641 aatcgaacac cacgatccgg tcggaatatc ggacggccag ctcagccagc aggttgttca
    50701 tggcgtcgga cgccagcagc tccgtcgcgc gcggatgggg cgtacccgcc ggcaacaggg
    50761 tgagcttgtc gatgttggtc ttgagcagca catcaccgag ttccacgtca tccgtggtca
    50821 ggacgtccat cagacccttc ttcggcggca gacccaggat gttcagaatg gatggacggg
    50881 atacgtcggc gtcgatcagc agcaccgtgt ggtcgagttc catggccatg ctgatggcca
    50941 gattgatgga actgaagctc ttgccctcgc cgggcaggga gctggtgacc atgatgaggt
    51001 ttgcactgtc taccgccgcc gcgcccttgc cctggatatt gcgcagcagc ggccgcttga
    51061 tgatccggta ttcgtcaccg atcagggaac gtggcgcatt gggcgtcacc acgccgatgg
    51121 acgcgaggcg ttcgagatcg atctcgacgc tgcgggcggt tgatgcgcgg gggcgggacg
    51181 acactgacgc ttcgggcgcc gcagacggaa ccggcaacgg cacctcggcc accggggcag
    51241 cctctacggt cacggcagcg gtcaccgccg cgatcacggg cgcaggctgt tcgaccggcg
    51301 tgccgaccgg ctcgatgcgc ccctcggccg atccggcaac cggtgcggcc tcgaccgtcg
    51361 caccggcgct ggcagccgcg gccgccttct gaagttgttc gagccgttca acggcctttt
    51421 cgataatgct catatccgcc tctcacgcga ccttggccag caacaaccag acggtcatcg
    51481 ccccgaacag ccccaccagg gcacctactc cgccgaggaa cacgatgcgg ccgcgacgtt
    51541 gcgtgcccat ccgctcgggg tccgcgatca tgctcaccgc gccgagaacc ggcagaccgg
    51601 tgacatcgcg cagaacctgc atgtcggtga acacgggacg gatctgactc atcatgaagc
    51661 tcacgacagc gcccagcagc aacgccgcca agccggcacc cggcatcagc agcaggcggt
    51721 tgggggccgc gggcttgctc ggcaaggtcg gtggatcgat caggcggaag tccgcgacac
    51781 ccgaggtcgc ccccatttcc acggccatgg tggccgactc gcggcgggcc acgagggcgt
    51841 cgtagttctt cttgttgatg tcgtggtccc ggttcagctg cgtgtattcg gcctcgagct
    51901 gcggcaccat gcgcgacgcg gacgacagat tctgctggcg cgcctcgtat tccgacacgc
    51961 gggccttcag ggacgccaca ttggcttccg cctcggctag ggacatcttc atctgctgga
    52021 acaccgggtt gctgttgatc gacccgacgg tgctcggccc ggccttgcgc cgggactgga
    52081 tttcctgatt cttctgggct tccagctgtt cgatcacctt gcgcgtgccg atcacgtccg
    52141 gatgttgatc ggtatatttc tgcagcaggg aatccagatt cttcttctgg gcctcgatgc
    52201 ggccatcgag ttcagggact gacaccgatg cgagggcgga actgctggga ttctccggca
    52261 gcaggacggg atcctcgcct gccagttgac gcttcagtgc gtcacgggaa ttctcggctt
    52321 cgcgcagggc gagacgggcc tgattgagct gttcgttcac ttcgcccatc ttcgagaaat
    52381 aatcccgacc gccagccccc atggtgctca gattgcgcag tttgaattcc ttcaggcggt
    52441 tttcggcctc ctcgagcttc tgctcgtagg tcttaatctg ctcttcgatg aacttgcgcg
    52501 ccgtatcgga atccttgcgc ttgtcgccga gaccggactc cacgaagatc gagacgaggg
    52561 actgcacgac acgcttggcg cgttccgcgt tggtgtcctt gtaggccagc gtgtagatgt
    52621 tgtcacgacc cgcctcgcgg atctcgaggg ccttggtcac gccgacgatc agggcctcgc
    52681 gctcttcctt ggtcttgacg gccaggtcca ggtcggccat gcggatcagc ttttccacgt
    52741 tggggcggct gatcagtgtg cgggaaagga tgttgatctg ctgttccacg ttgggctgca
    52801 cggcaagccc cgacatcagc ggccgcagga tggactgggt atccacgtag acccgcgccg
    52861 aagactcgta gcggtcgggt agcagataca ccgtggcggc cgcaatcggg ccgacgatcc
    52921 acgccgccgc gagcccccac cagcggtaca gccacatgcc gcggaggatg accgacagct
    52981 gtcttaagat atcttccatc aggaatttcc ggtagcgctc cccggcctgc cggccaggga
    53041 gtcagacatg agaacgatca gaaccagctc tgggggatga tcacaacgtc gccgggcttg
    53101 acttcggcgt tggcggacac gtcgccgcgc ttgatgaggt cacgcagacg cacgccgtat
    53161 tcggcgttgt tgtcggaggt ccgcaggatc ttggcccggt tgccgtccgc gaaatcggtc
    53221 agaccaccca ccgcgatcat cacgtcgagg agcgtcatct tctgcttgta cgacaaggtc
    53281 tgcggccggg ccacttcgcc cacgacccgc acctgctcgg cgtaagggcc gacaaagtcg
    53341 gtgacgatga ccgtgacgac cgggtcccgg atgtacttgg aaagcgcctt ttcgatctcg
    53401 cgggcaaggg tgctggagtc acgaccggag gccggcaggt cttccaccag cggtgtcgtg
    53461 atcttgccgt cgggacggac cggcaccgtc atggacaatt cggggttgcg ccagaccacg
    53521 atgttgacct tgtcaccggg gccgatcaga tagttgtact cgggcgtggt ggctgtcgcc
    53581 ggcggaggcg gcagtttggt ggcgcagccc gttgcaagga ccaccgccag ggtcaggaga
    53641 cccgtcgtca cgatgcgttt catagactac ttcccctgta catggcccta aatcggcctg
    53701 gaatgataac gagcggatat tacggcagag aacagcgctt cccggagaaa aacgtcacga
    53761 gcaacaaaac ctttaatgca cgtgctatta catccgggtt gggaagccta tccagccggc
    53821 aagcgggcca tcgcggcccg cccggggacg ccggctcaga acgaccagcg ggcctgcgtg
    53881 taggccctgt cgcgcgtgtc gtacatgccg aagagcccgg tggattggcc accgaagctg
    53941 tccacgccaa acacgacctt gaggttgcgg ctcgcggacc agacgagttt gggccggaac
    54001 atgaagtcgg tacgattgac actggagacg aacagggctt ccgcctccca gttgttggag
    54061 agcttggtgt tcagcagcac gctggcgccg ccttcgatcc gatcctgacc gattcggggg
    54121 tcgaaatcca ggtaacgccg ggcgaaatac tggagattca cacgcccgtc ccggaatgcg
    54181 gggatgtcca ccccgacgac gtaatccagc gtgttctggg caatcagacc gaagtctacg
    54241 gtgggatcga gggtgttgaa ttcgcgcccg cgggtctgca ccagttcgcc cttcagcaca
    54301 aaggcgccga gatccttaga gaccgtgccg cccgcctgac tgatgcgcag gtgacgcggc
    54361 tcgaacaccg gagaagtgat gctgcccaga cggaagaagg tctgcgaaac gtccatgctg
    54421 cgatagtaga agccggacac atcccagcct gcggcaaggg tggacagcct cccgccccag
    54481 ttggtattgc ccagggaatt ggacggtttg acctccccca gcaccggcac gccggccggg
    54541 atcggataga actccgcgcc aggcttgccg atccggtcat aggacggcac cgggatccag
    54601 atcagctcgg cgtgggtatc gccgaagtag tactcgcccc gcgccgccca ctgcggaatc
    54661 cgcaactggt cgaaatcggg caggtaaaac gtccgcagat cccgcgcaga cacgacatcg
    54721 gcgaagaaca gtccgaccat ctcgccccac acgatgtgct gacggccgag acgcacatcc
    54781 acatcaccga ccgagaagtc cacataggtc tcgcgcagat cgaagcccag ttgctgatca
    54841 cgacgcaccg catccgggta gaacccattg tcgatggcga acaccgcgtc ggcgtccgcc
    54901 cgacccgaga tcttccactt cagaccagcg ctgccaccgg tcgcccccaa ttccagacgc
    54961 gagcgggcca tggaccagtg ctcggggctg gcataggtgc gcgccaggtc gaactgcagc
    55021 acacccgtcg tgcgtgcccc ctcgtccgcc acatccaggc ccgcatcgtc cccttcttca
    55081 tcagcgtgga caggcgtgcc ggtgcacagg gccagtgcgg ccagaagcgc cgcggcattg
    55141 cgggcgagcg tcgtcgtttt cataggattc aaatctccgg atcatcttcg ggcttgcgcc
    55201 ggtcctgcac gaaaatccaa ctgccctcac gtttcatgcc gagagcggta atctggccat
    55261 cttccatccg gacgagacgg tcggcccggt tcatgacccg cttgtcgtgg gtcgagaaga
    55321 cgaaggttgt gcccgcattc tgattgatct cacgcatcag gttgaggatg ctctcgccgg
    55381 tccgccggtc caggttggcg gtcggctcat cggccagcac caccctggaa tgggtgacca
    55441 gggctcgggc aatggccacc cgctgacgct ggccgccgga gagctgattc ggacgatgat
    55501 cagcatactt ggtcaggcca accatgttca gatagaacgc aacccgctcc gcccgctctt
    55561 ccttggtcag ttcggtcatc tgcaggagcg gatattccac gttttcctga gcggacaata
    55621 ccggaagcag gttgaacgtc tggaacacga aacccagggt gcgtgcccgc acatccgcca
    55681 gactgtcggg cgtctgcccc gagacgtcga tgccttcgat ttcgacgcgc cccttgctcg
    55741 gcgtatcgat gcaccccatg atattcagaa gtgtcgattt gccgctaccg gacggacccg
    55801 cgatggccat gaacaccccg ggctccacca cgagggacac accctccagg gcggtgaccg
    55861 tctgttcgcc gaggagatag gtcttactga cgttttcgac acgaatgatc gacatgtacg
    55921 cgctgcacta tgttgtttgc ttgcgaagga taacaccgtg acccctcaaa atcccctctg
    55981 gccccgccgg aatctgctct tccgcctcct gccggcagcc ctgctggccc tttccagcct
    56041 gcccatccat gcggaagacg acgaattcgc gaaaaacctc gtcgccaagg ccgatgaagt
    56101 gcgctttccc cgcgaaggat tccaggtgga catcatcgtg gattcgacga gcccgggcga
    56161 agatccggac cagcacaagt accggattct atccaaaggc aatgagaaca cgattgtgca
    56221 gaccctcgag ccggcgtccg atcggggcca ggccatgctg atgcggggcc gcgacctgtg
    56281 ggtctatctg cccaatgtct cgcagccggt gcgtctgtcc ctcgcccagc gtctgacggg
    56341 acaggtcgcc aacggcgatc tggcgcgcgc caacttcgcc ggtgactatg atccgaagct
    56401 gttacggact gacacgctgg atggcgacaa ggtgcacgtc ctggaactgc gtgccaacga
    56461 ccgcagcgtc acctacagca aggtgctcta ctgggtgcgc gcctccaaca attacccggt
    56521 cagggccgag ttctactcgg cctccgaccg cttgatgaag acctgtcgct tcgagaagta
    56581 ccagaccctg ggcggtcgtg cccgcccaac ccagctgatc atggaggacg cactgcgcaa
    56641 gggcgacatg tcggtcctgc attactcgca catggcgctc aaggatctgc cggaccgcat
    56701 tttcaccaag cagtacatgg acaaactgaa tcgggtcgat taacccgatg cgccccttca
    56761 ggccagcgtg acaggggctt ccccggcggg cggagcgatg cgaggcaggc cggcgtacag
    56821 cgcctgccgc aatgcctcgg caccgcccgc cggttcgtcg agatccagcc tgcgcaccac
    56881 gccggcaatc cgcaggtcgg cgccgtccgc atcgacaccc aggatggtca cgccggccgg
    56941 cgcagtgcgc gccaattcgg cggtcagctc gcgctccacg gtggcagttg gctgccagcc
    57001 cgtcgcaggc gcgtcacacc agcccatgcg cgcgaagcct ccgatcagac gtacgcgctc
    57061 gatgcccatc cggaagaagc ggaaatcccc gagctgcaga taatctgccg cttccggccg
    57121 gtaggacaga taacgttcgc gcgccgcggg ctcgagatcg cagggcacga catgccccat
    57181 gagggtcgcc cgcggctgct cgaggggcga cgtcgaggtt tccgccacca gcagactggc
    57241 ccgtgaatcg gccagcagat tgcgggtgtg ttcggccaga gcactgatca ggaagagcgg
    57301 cgcgccacca ccatccaggg cgtaaggcaa caaggtgaca aaagggtagc ccggcatcgt
    57361 caccgaccgg gttccaagcg cgccctgatt gcaggaccgc aggagcgccc ggacttcccg
    57421 cggatcgact tgagtcacgc ccacctcagg cgccccgctg gcggcgtgtc tcgaacagac
    57481 agatcccgct cgccaccgac acgttcaggc tttcgacgga gccgtacatg ggaatcctca
    57541 ccagctcgtc gcaggtttcg cgcgtgagcc gacgcagacc gtctccttca gcgcccagta
    57601 cccaggccac ggacgccttc tgctccacct cgtaaaggga tttgtccgcc tcgcccgccg
    57661 taccaagcac ccacacgccg gcttcctgca tctcccgcaa ggcgcgggcc agattggtga
    57721 cggtgatgta aggcacggtg tccgccgcac cgctcgccac cttgacggcc gtggcgttga
    57781 ggccgaccgc ccggtctttg ggcgcaatga ccgcgtgggc accggcagcg tccgccaccc
    57841 gcaggcaggc acccagatta tggggatcct ggatgccgtc caggaccagc aggagggctg
    57901 cgccgtcgag gctgtcgagc aggtccggca gcatgacctc ccgccgccgg ccatcgatgc
    57961 gcgcgacaac gccctgatga cggcgcgtgc cgaccatctt gtcgagacgt tcgccttcgg
    58021 agggaatcag gcgcacaccc agttgctcgg cctgacggag gaagtcccgc gcgcggccgt
    58081 ccgaacgctg ggcgtcgatg tagatttcga aaaccgcttc cggatcgtga cgcagcttgg
    58141 ccaggaccgc gtgaaaacca tagatgagac gggaactggc ggattcagac acgggatttc
    58201 gctttcttct tggtgccgcc ggctgcgcgc ttggacgggc cggtggaggt ggaagcccgt
    58261 gatccggcag gcttggagga cgtcttggtc ggcttggagg gtttcgatgc cttggcggcc
    58321 ttggacggtt tggcggcctt ggacgtttgc accgacttgg caggcttgac cggcgaaccg
    58381 cccgcatccc cggtcttcga ggccgaccga gcccgacgcg gcgcggtctg gaccggtgac
    58441 tccggggaca cgatcggggc catgacctcg gcacccgcag tcccattggc cacacgccgg
    58501 cgccgggctg gcgcgggcgt ctcggcggcg gcatcaccgc gctcggcctc gggctgccgg
    58561 gctggcagtg ggccttcgag caggcggaag tcgatcttcc cggcttccat gttcacccgc
    58621 accagttgca cgcgcacccg gtccgacagt cgataacgct tgccggtccg ctcgccgacc
    58681 agggtgtgat gggcatcgtc gaagtgatag tagtcgttac ccaggtcaga gatgtgcacc
    58741 aggccttcga cgaacacgtc atccagggcg acgaagaggc cgaagggcac gacggcggac
    58801 acactgccga tgaactcctc gccgatgcgg tcacgcatgt agtagcactt gagccagttc
    58861 tccacgtccc ggtcggcctc gtcggcccgt cgctcggtca tggagcaatg caggccgatg
    58921 gcctcccact cgcccgattc ctgcggcggc tgatagcgtt cgccggtgag caccgccttg
    58981 atggcacggt ggaccaacag gtcagggtag cgccggatcg gtgacgtgaa atgggtgtag
    59041 gcctcgtagg ccagcccgaa gtgccccaga ttgtccgggc tgtaaacggc ctggcgcagg
    59101 gagcgcagca tgaccgtctg gagcagttgc aggtccggcc ggccctgcag gctggccagc
    59161 agccgcgcgt aatcgcccgc gtggggatcg tcgccgccgg ggagctgaag tccgaaggat
    59221 cccaggaatt cccgcaattt cacgagcttt tcgggcgtcg ggccttcatg gacccggtac
    59281 agcgccggat gctcgtggcg ctgcaggaac tccgacgcgc acacgttggc ggccagcatg
    59341 cactcttcga tcaggcgatg ggcatcgttg cggctttcgg gcacgatctt ctcgatcttg
    59401 ccttgatcat cgaagatcat ccgcgtttcc tgcgtttcaa aatcgatcgc accccgcttg
    59461 gcccgcgcct tgaggagcac ccggaacagc ttgtccaggt tctgcagatg gggcagcaag
    59521 gcaccgacgg tctccagcgc cgccggatcc tgatcgtaaa gggcagccgc cacctgggtg
    59581 taggtgagcc gcgcgtggga gaacatcacc gccggataga agcggaactg cttgatcgtg
    59641 ccggtctggg tgatggccat gtcgcacacc atgcacaggc gctccacctg cgggttgagc
    59701 gaacacaggc cgttggagag cttctccggc agcatcggga tgacccgccg cgggaaatac
    59761 accgagttgc cccgctcgta agccgaatcg tccagggccg agccggggcg cacgtagtgg
    59821 gagacatccg cgatcgccac caccagccgg aaaccccgtc cctggcgctc gcaatagacg
    59881 gcgtcgtcaa agtcacgggc ggtttcgccg tcgatggtca ccagcggcag gccggtgatg
    59941 tcctcgcggc ccttccagtc ggtcttgcgc accttgtccg gcaggcgccg ggtctgctcc
    60001 ttggcggcgg acgggaactc gaaaggcaac tcgtgcttgc gcagggcgat ctcgatttcc
    60061 atgccgggat cggcgtaatt gcccagcacc tcgacgacgc gcccgacagg ctgggcgtag
    60121 cgggtcggct gctggaccag ttccaccacc acgacctgac cggccgtcac cttggccttg
    60181 ccagcggagg cgagcaggat ttcctgggcg atgcgacggt tttccggcac caccagcgtg
    60241 accccgttct ccacaagcac gcgcccgacg atgcgggtgt tggcccgctc gagcacctcg
    60301 acgagcttgc cttcgggccg cccgcgccgg tcggtgccag caatgcgcac aagggcccga
    60361 tcaccatgca gcacctcgcg catctcgttg gggccgagga agatgtcctg cccttccccg
    60421 tccggcacta gaaaaccgta gccgtccgga tggccctgga ccgtcccacg gatcagatcc
    60481 gccttgttcg gcagcaggta ggcattgcgc cggttgcgca ccagctgacc atcccgctcc
    60541 atggcgaaga gacggcgttc gaagaattcg cgctcatggg acgcgatgtc catggcatcg
    60601 acgaggtcag ccaggctcag gggcacgccg cggtcggcga gcacctgttc gatgaactcc
    60661 cgactcggga ggggaaattc gtaagttgcc acttcccgct cgtaaaacgg gtcgctgcgg
    60721 cggaatgcac tcagtttttt gggtgtcttg ggtttaggca ttgacaggga tgattgctct
    60781 tctataatgg cgtcttcgtt gcccagatgg cgaaattggt agacgcgcta gtttcaggta
    60841 ctagtgccgc gaggcgtgga ggttcgagtc ctcttctggg caccagagat attcaagaga
    60901 gagccggtcg attcgaccgg ctttttcttt gtgtgtgttg acaagcaggg gcgcgttctg
    60961 aaggacagga aacagcgcaa aaggattgca ggatcgacaa gccggaccgc ggagagtttt
    61021 ccgcgtgccg gctttttttc attgtggtcg tcgtcgatct caggcgccga agggatggcg
    61081 cttgaggatc gtctcatccc gctccggacc cgtggagatc acgtcgatgg agacttcgca
    61141 gatttcctcg atgcggtgca gataggcccg cgcttcaggc ggcagcgcat cccaggtacg
    61201 ggcaccgaag gtgctgccag tccagcccgg catggtctcg agaatgggtt cgcagcgaac
    61261 gacctcatcc gcccccatgg gaagcaggtc aacgcgcttg ccgtcaagca tgtagccggt
    61321 acacagcttg agctcggtga ggccgtcgag cacgtcgagc ttggtgatgc acaggccgga
    61381 cacgccgttg atgatgatcg aacgcttcag agccgccaga tcgagccagc cacagcgccg
    61441 cttgcgcccg gtgaccgtac cgaattcccg accgaccgtg gacatctggt agcccggcgt
    61501 acccggcgtc tcgaaatcca gttcggtggg gaacggtccg ccgccgacac gggtgcagta
    61561 ggccttggtg atgcccagca cgtagtgcag ccggccggga ccgacaccgg aaccggccgc
    61621 ggcctgcccc gccacgcaat tgctggacgt gacgaagggg tacgtcccat gatcgatgtc
    61681 cagcagcgtg ccctgagcgc cttcgaacag caggttgccc cccgccttgt tgagggcgta
    61741 gagatcggct gacacgtcgc agaccatggg tttgatctct tccgcgtcgg ccatggcctg
    61801 gtcgtagacc gactggaaat ccaccggatc ggcgcccagg tattgggtca gcacgaagtt
    61861 gtggtactcc agcagttcct tgagcttctc ggcgaaacgg gccgggttga acaggtcgta
    61921 gacccgcagc gcccgccgtg ccaccttgtc ttcgtaggtc gggccgatgc ccttgccggt
    61981 ggtgccgatc ttatcgccag cactcttgcg ggcttcccgc gcacgatcca ccgccgcgtg
    62041 ataccccagg atgatcggac agcccgggct gatgcgcagc cgctcacgca ccttgatgcc
    62101 accagactcc aggccgcgca cctcggacag caggtgatgg atatcgagca ccacaccgtt
    62161 cccgatgaag cacttcacgc cctcgcgaac gatgcccgac ggcaccagat tgagcttgta
    62221 ttcattgctg cccaccacca gcgtatggcc cgcattatgg ccgccctgaa agcggaccac
    62281 gccttcggcc tgatcggtca gccagtcgac gatcttgccc ttgccctcgt cgccccactg
    62341 ggtgcccacc actacaacgt tctttgccat gcgttcattc tccttgaagc ggttcaacct
    62401 gccaggcacc gccccgcaga accagtcgcc ggtcacagcc ggcctcacgc cacgtgccgt
    62461 catggccggg caattcaatc acgacgatct cgcccgacgc acgcagccgg ccgacctcct
    62521 gggcaaatcc cggcgccgag cactcgccgg cgaggatcat gccccgccct gccccgtcgg
    62581 gcgtctgggc cgccagttcg cgcagatcca ggctgaagcc cgtggccggc cgaccacgcc
    62641 cgaaggccgt cccgacccgg tcgtagcgcc cgccctgggc gagggccacc ggcgaggtgc
    62701 cgccgtacgc ggcaaacatc acgccgctgt ggtagtggta accgcgcagg tccgccagat
    62761 cgaagctgat gggtagatcg gcgagaagtc gcgacagcga ctccagatca tccagtgcgc
    62821 cccgaacatc cgcatcatcc aacccgctca aaaccccacg ggcacgctcg agcacttcgg
    62881 gcccgccata cagggtcggc agcaccagca gcgcggagcg gaccgcctcc ggcgcgtcac
    62941 caagaagatc acgcaagccg ggaatgtcct tggactgcaa acaatcgaac aacgattcct
    63001 gctgctcgtc ccgcagccct gcccgcgccg ccagggcacg gaacagtccc acatgaccga
    63061 gatcgatgcg tgacgtggtg acgcccgcaa tctgcaagga ctcggccagc agccggatca
    63121 cttccgcatc cgcctcgagg ccggcatgac cgtaaagctc ggcccccaac tgaaggggct
    63181 cgcgggtcgc cgtcaacgaa gacggcaagg catgggccac actgccgcaa tagcacagcc
    63241 gtgcaacgcc ctgccggttg agcaggtgcg cgtcgatgcg tgccacctgc ggcgtgatgt
    63301 cagcccgcac gcccatggtc cggccggaga gctggtccac gagcttgagc gtcttgagct
    63361 gcagatcctg accggcaccg gtcagcaggg attcaagata ttcgagcagc ggcggcatca
    63421 cgagctggta gccattgagc cggaatgcat cgagcagacg gcaacgcaga ctttccagct
    63481 tttcggcctc tgccggcagg acgtcctgga tatgttcggg tagaacccaa cgcaacatgg
    63541 gcatggtgtc tcaggaaaaa acgatcagta cgatcaggcc gacgagcatg gagctgagtc
    63601 cgatgaaacg gatctgcccg tcggacatgc gcacgagacg ggtgaaggtc tcgcgccaca
    63661 tcttgggcgc cacaaagggc aacaggcctt caagcaccag catcagtgcg aaagccagaa
    63721 acagggagga tcccattatt ttgcgctctt gccgcccttg ttcgcgtccg ccggtgcggt
    63781 cgccgtgcct cggccgccgg cactcttcat gtacttgaag aaatcggcgt tgggctcgag
    63841 cacgagcacg tcggacttgc tcttgaagct gttccggtag gcatcgagac tgcggtagaa
    63901 ggcaaagaac tccggattct ggctgaaagc ctgggcgtag atcgcggatg ccttggcatc
    63961 gccttcgccc ttgaccttct gggcttcgcg ataggcctcg gcgacgatga cttcgcgctg
    64021 cttgtcggcg tcggcacgga tcttttcggc gtcggcggcg ccctgggagc gcagttcgtt
    64081 ggccacccgc ttgcgttcgg cttccatgcg gcgatagacg gactcggaca cttcgagggg
    64141 cagatcgacg cgcttcaggc gcacatccac aatctgcacg ccgatcttgc gggcatcctg
    64201 atcggccttg acgcgcatct cgtccatgat cttctcgcgc tcgccggaga cgacctcatg
    64261 aaccgtacgc ttaccgaact cttcacgcag gcccgcattc acggtctgct caagacgggt
    64321 cttggcccgc gactcatcac cgccgacgga aatgtagtag agcttgggat cgacgatccg
    64381 ccacttgacg aacagatcca ccagcacgtt cttcttttcc gaggtgatga agcgctccgg
    64441 ctcattggaa tccatcgtca ggatgcgccg gtcgaaatag cgcacgttct ggatcaaggg
    64501 aatcttcacg tgcagaccgg gctccgtgag ctggcccttg acctcgccaa gctggaacac
    64561 cagagcgtac tgacgctggt caacggagaa gagcgacatg gacatcagcg ccaccacgaa
    64621 gagcagaacg ccgatgaaaa agggtgcgcg gtcacgcatt agcggtctcc cctctcgcgg
    64681 ttacgcatcg cctcgcgcgt gcgggattcg ccggacagcg cgtcgggccg tggcggtgcc
    64741 gggctattgt ctgcggcaac ggcagcggca ggcacctggg gacgcgtctg ggcggcagcc
    64801 tccggtgtta ccgcgccgga cgcctgagcc gcggtggcgg caatcagctt gtcgaggggc
    64861 agataaagca gattcccgct ccctttgctg tcgatcacca ccttcgaact gttggacatg
    64921 atctgctgca ttgtttccag gtacatgcgc tgacgcgtga cctccggcgc cttggcgtac
    64981 tcggtgtaga tctggcggaa gcgcgacgca tcgccctcgg cctgcgcgac cacccgcccc
    65041 ttgtacgcat ccgcctcttc gagcagacgg gacgccgtgc cgcgggcacg gggaatgaca
    65101 tcgttggcgt aggcctgccc ctcattcttc tggcgctccc ggtcctgccc cgccttcacc
    65161 gcgtcgtcga acgctgcctg cacctgctcc ggtggctggg cattctgcat ggtgaccttg
    65221 ctgatctgga tgcccgtggc gtagcggtcg aggatgtcct gcatgagctt gtgggcattg
    65281 gcggccacct gctcacgacc ttcatagagg acgaagtcca tcttgctctt gccgaccacc
    65341 tcacgcaccg cagtctcggc ggcctggatc acggaatcgt cggcgttgcg gttgttgaag
    65401 agatattgct gtggatcctt gaggatgtac tgcaccgcga actggatatt cacgatgttt
    65461 tcgtcatccg tgagcatcag ggcttccttg agcaccttgt tgcgctctga accgcgatag
    65521 ccgatctcga cggtgcgcac acccgtctgg ttcacgagtt catgggtctg gaccgggtat
    65581 ggcagccgcc agcgcaggcc cggctccgtc gcctcgacga agcgcccgaa ttgcaggacg
    65641 atgccgcgct ggctggcgtc tacgatgtag aaaccgctgg ccagccagat caccaccgcc
    65701 aacgccagga tcaggccaac gcccccgccg aactggcgga aattcagttc gggcatccgg
    65761 tcgccgcccc ccgaatcacc gccgccgtca ccgccacggc cgggctggcg cccgaacatt
    65821 ccggacagtc ggcgattgaa gtcccgccac aactcttcca gatcaggcgg cccctgattg
    65881 tcaccgccac cgcggttgcc ccacttcggg tcgttgatag acatgaagat gcccgtgatc
    65941 actgtgaaag tccgctccgg gaagaaagcc gggattcagc ccagttgagg ctgatccgaa
    66001 ggtgcggcga gtgccgcctt gtgagccagt gcaacgtcgg ccaggatctg gcgcaacaga
    66061 tccagaccct cgcctgtcct tgcgctcagc agaacccgcg agattttacc ataagcgtcc
    66121 acctggacct ccggactggc cagggtggca tcgatcttgt tccacaccag gatctgcggg
    66181 atgtcgcctg caccgatctc gtgcagcacg ccattgaccg catcgatctg cccgtcccgg
    66241 tcgtcgctgg ccgaatccac cacgtgcagg agcagatccg ccgcgacggt ctcttccagc
    66301 gttgcatgga atgccgccac caatgcgtgg ggcagatcgc ggatgaagcc gacggtatcc
    66361 gacagcacga tcgtaccggc ctcgtccccc aggtacaggc gccgactggt cgtatcgagg
    66421 gtcgcgaaca actggtcggc cgcataggca ccggccttgg tcagggcgtt gaacaaggtg
    66481 gacttgcccg cgttggtata gccgaccagg gatacggaca gcacatcccg acgctcccgt
    66541 gcccgccgtc gcactgcgcg ctgacgctcc agcttgcgca gacgatcctt gaggagcttg
    66601 acccgctcgc caagcaggcg ccggtccgtc tcgagctgtt tttcgccggg accgcgcagg
    66661 ccgatcccgc ccttttgccg ttccaggtgg gtccatcccc gcacgagacg ggttgccatg
    66721 tgctcgagct gggccagttc cacctgcagc ttgccttcat gactcttggc gcgcagggcg
    66781 aagatgtcaa ggatcaacgc ggagcgatcc acgacccggc aattcaggcg cttttccaga
    66841 ttgcgctgct gggccgcgga gatgtcgtgg ttgaagatga caagatcggc ctcgttcatg
    66901 cgggccagca ggccgatttc atcggccttg cccgttccga cgaagtgcgc cggatcgggc
    66961 gcctgacgcc ggccctgcac gacccctacg acgcttgcgc cggcgctgga ggccagcagg
    67021 ctgacttcgg cgaggcgctc gtcaataccc ccctgcccga aatccacctg gacgatgatg
    67081 gcgcgattgc ccgactcggg gcgatcaaac atgacggacg ccctggaaac ggagttgccg
    67141 atcagccgtg atacgcgccc tgaggtgaga aggtttcaga ctcagcttgc agcctcgttc
    67201 tgttcctgct ggatgttcac cggacgggct ggcaccaccg tggaaatggc gtgcttgtac
    67261 accatctggg tcacggtgtt cttgagaagg acaacatact ggtcgaagga ttcgacctga
    67321 ccctggagct tgatgccatt caccaggtag atcgaaacag gcacatgctc acgccgcagg
    67381 gcgttgagga atggatcctg gagaagttgt cccttgttgc tcatagtgtg aacctcttgg
    67441 tttttagttt gaaataatta taaagccatt tgctgcaccg ccgcatgacg accgctctgg
    67501 cttggtgtgg tgtggaactc ggtgcgttca atcacgatcc gcgtagggat tgtgagacga
    67561 cttgaactgg atgcgcaacg gggtcccctg cagtttgaac gcttcctgga agcttcgttc
    67621 cagatagcgg acgtaggacg ccggcacatg atccagcgca ttgccgtgaa tgacgatgat
    67681 cggaggattc atgccgcctt ggtgagcata acgcatcttc ggccggaagc ccccgtggcg
    67741 cgggggctgc tgcttgacga tggcatcgag caggaggcgc gtgagcttgg gcgttgcaag
    67801 cttggtcatg gctgcggcat acgcgccatc caccgaccgg agcaggggcg gaatcccctg
    67861 ccccttcagc gcagagatgt acagaaaccg ggcaaatccc aggaaaggca gcttgcgggc
    67921 aatgtcctgc ttgatgcgtt cgcgacggta gtcgtccacg tcatcccatt tatttactgc
    67981 caccaccagc gcgcgccccg cctccacaac gaatccgccg atgtgggcgt cctggtcgga
    68041 gatctcctga ctcgcgtcga gtacgaggac cgccacattg cattgctcga ccgcctgcag
    68101 ggtcttgatg accgagaact tctccacggc ttcgaaaacc ttgccacgcc ggcgcaggcc
    68161 ggccgtgtcg atcagcgtgt agtgcttgcc gttgcgctcg aaaggcacct cgatggcgtc
    68221 gcgggtggtg ccgggcatgt cgaaggcgat gacccgctcc tcacccagca gcgcattgac
    68281 cagcgtggac ttgcccacgt tgggccgtcc gacgatggcc acgcgggggc cctgggtcgg
    68341 cgcctcgtcc tcgccgccat cggacgggaa ggtgtccacc accagttcca tcagcgcctt
    68401 gacgctgtcg ccgtgggccg cagagatgca atagggctcg cccaggccga gagcgtgaaa
    68461 atccagcgag accatcgccc ggttcatgcc ttccgccttg ttcaccacca gaaaggtggg
    68521 gcggccggcg cggcgcaggc gaatggcgat ctgttcgtcg tgaggcgtca gcccgctgcg
    68581 cgcatccacc atgaagagca gcgcgtcggc ttcggcgatg gcctgctcgg cctgcttggc
    68641 catctcatga acgatgccgt ccttggcaac gggatcgaag ccggcggtat ccaccaccag
    68701 gaactcacgc tcgtccacgc gaccgaggcc gtagcgacga tcccgcgtca ggccgggaaa
    68761 atcggcgacc agcgcgtcgc gggtcttggt cagacggttg aaaagggtgg acttgcccac
    68821 attggggcgc cccaccagca ccagcgtcgg tttcacgtcg tcgccactcc tactgcacgc
    68881 caaaggcgaa gacgctgccg tcgcgggtct gcaccagcag atcgctgccc acacggcgca
    68941 gatccgccag caccggactg ccatcggtac gctgacgacc gacgaaggtg ccatcgtcct
    69001 tgcgcagcac gtgcacgatg ccttctacgt cggccaccgc caccatgtcg cggaaagcca
    69061 gcgggcggcc cggactgcgt ccggcgagct ggtcctgctt ccacatgctt gcaccgctgg
    69121 tgaggtcgag ggcatggacg gcgcccttgt cgtccgtcac gaagacatgg ctctcgtcga
    69181 gatcgagacc gcgcgcgctg gaaagatcac gggtccacaa ggtcgaaccg ttggccagat
    69241 cgaagcaggc cacgcggccc tggtaggcca ccccacaggc agcgcggccg ttcaggaccg
    69301 gcgggctgga gatgtcggcc acgcgctcca gttcggtcgc accacgaggc accgacaccg
    69361 tcccttccca catgagcgca ccggtgttga cgttgacggc cgcgagcttg ccgccgggaa
    69421 acccggcgaa gatcgcgcga tccgccatca ccacgcctgc cgccgaccgc agcgcaaggg
    69481 ccggtgtcgc gcgctgatag acccagcggc gcttgccgtc cgcaggctcc agtccgaaca
    69541 ggcggctgtc cgccgcgcgc acgaccacga caccgtcccc cagggcgggc gcggccagca
    69601 cttccgcatt cacgttggcc tgccagcgta ttgcaccgct ggccggatcc agtgcgtaaa
    69661 cctctccctt cgggctggcc accagcacca tctcggcgct tccgccaaca ccgcccgaca
    69721 gcccggtctt cagcgacacc ttccacacgg tgcggccatc ttccacgcgc accacggtcc
    69781 cgtcggcagc cgcggcataa actgcggagc ccacaaccag cggcgagaac accgacgtac
    69841 ccgcgcttcc gacccgcagc ttccagtctt caccaagctt gacgctgtcg gtgatcggct
    69901 tgagcggcgg gatcttgggt ccgctgctcg aaaaaggatt cagcgaactg cagcccaaca
    69961 ggccgaacgc cgcgatcagg gccgcgacac gcccgaggcg cctcactggg cacctcccag
    70021 accgtccagt ttggcctgga ccacttcctt gaacgcgacg gcttcaggct tggtagccga
    70081 ggccagcgcc tcgagcgcag ccttgtaggc cccacgggca tcggccacct tgccctgagc
    70141 cgccaggatg tcaccgcgca gatccccgta gcgggcctgc agcgcctcgg agggcttggc
    70201 ctgcaactgg gccagcgcct cgtcatagga cttctcgtcg agcaacacgg ccgccagacg
    70261 cagacgggcc aattcacgca gcgcaggatc gttggccttc tcggccgccc agccaagctg
    70321 cgcacgggcg ctcttcaggt caccggcatc ggcctggacc cgcgaagaca gcagcgcccc
    70381 cagatcggcg taggccgtac cgctgtattt ctcgatgagc atgccggcag cttctcgggc
    70441 ctgctgcggc ttgttctcgg ccgcggcctt ctgcaccgcc gcgtacagca ccgacgcctc
    70501 accgctctgc ttaccctggt accagttcca gccctgccag ccgacggccg ccacggccgc
    70561 cgccagcgca atgcccgtca ccaggttgcc atacgtgtcc caccaggcct tgatctggga
    70621 gatctgctcc tgctcttcga ggtcataggc cgccatcgtc gtcctccagt tcaagcaggc
    70681 gatctgccag ggtttcggga agaatttcca ttggaattcg gatctgttca ccgccactgc
    70741 gcaggggctt gaggctcacc tcgcccgctg cggcttcgtc gtcaccgatc accacggcgt
    70801 aggtggcacc ggaggcatcg gcgcgcttca tctgggactt gaaactgcct ccgccgcaat
    70861 gcagggtcac cgtgaagccc gtcccacgca gggcttcggc ggcgcggaag gcggcacgct
    70921 gggccgcttc gccggcatgc acgagataca cgtcgggcgt caggggttcg ggaatgaaac
    70981 cgctctcctg ccacagcacc agcaggcgct cgatgcccat cgcaaacccg caggccggcg
    71041 ctggcttgcc gcccagctgg gcgatcagcc cgtcgtatcg accgcctgcg cagatcgtgc
    71101 cttgcgcacc caggcgggtc gtgacccact cgaacacggt gcggttgtag taatccaggc
    71161 cgcgcaccag ccgatggttg atgcgatagg ggatgcccgc atccttgagc agggcctgca
    71221 cgccatcgaa atggcgcagg gattcgtcgc ccaggaagtc catcagcctg ggggccgctt
    71281 cgacgatgtc ctggaggtcc ggatttttgg tatcgaggat acgcagcggg ttggtatgga
    71341 gccgacgctg gccgtcctcg tcgagccgtt ccttgtagcc ttcgagatag gcaatgaggg
    71401 cggcccggtg ggcggcccgc tcgtcggcgc tgcccaggga gttgagctca agcttgacgt
    71461 cttcgaggcc caaatcgtcc cacaggcgcg cgcacatgag aatgtgctcg gcgtcgatgt
    71521 ccgggccatc gaagcccagc gcctcgacac ctacctgatg gaactggcgg taacgcccct
    71581 tctggggccg ctcgtggcgg aacatggggc cgtggtagta cagccggcgc ggctggccga
    71641 agatcagact gtgctggatc gccgcccgca cacaggaggc ggtgccttcg gggcgcaggg
    71701 tcaactgttc gccgttgagg gcgtccacga aggagtacat ctccttctcg acgatgtcag
    71761 tgacttcgcc gatcgcgcgc ttgaacagtg gcgtcggctc gacgatgggc atgcggatgg
    71821 gacggtaacc gtagctgcgc agccagtcgc ggacgatgtc ctcgaactgc tcccagactt
    71881 cggcatcgtc gggcaggatg tcgttcatcc cgcgcacggc ctggagggtc ttgctcataa
    71941 agtgcagtct gcgtcgcgac ggtcaggccg ccgcgccttt cttgggataa gtgcgggcca
    72001 cgtaacgctc cacgatggcc ttgaattcgc cggcgatgtt gtcgccgcgc agggtcaggg
    72061 tcttgacccc gtcctcgaac accggcgccg ccgggctctc gccggtgccg gggagcgaga
    72121 tgccgatgct ggcgtgcttg ctttcgccgg ggccattgac gacgcagccc atcacggcga
    72181 gggtcatgtt ctccacaccg tcgtactgct cacgccaaac gggcatgttg tcacggacga
    72241 aggactgcac gtcggaggca agctcctgga acagggtgct ggtggtacga ccgcagcccg
    72301 gacaggccgt caccatcggc gtgaaggcac gcaggcccat ggtctggagc atctcctggg
    72361 cgacgatcac ctcctgcgtg cggctgccgt tgggctccgg cgtgagcgac acgcggatgg
    72421 tgtcgccgat gccttcctgg agcagcacgg ccatcgccgc ggtggacgcc acaatgccct
    72481 tggagcccat gcccgcttcg gtgagcccca ggtgcagcgg atagtcgcag cgggccgcaa
    72541 gatcgcgata cacggcgatc agatcctgca cgctgctgac cttggcggac aacacgatct
    72601 tgtcgccggc cagcccgtac tcctcggcct tggccgcgga ctccagggcc gacgtgacca
    72661 gcgcctcgcg catcacggcc cccgcatccc gcggaacggc gcggcgcgcg ttctcgtcca
    72721 tgatgcgggc gagcacggac tggtccaggc tgccccagtt cacgccgatg cgcaccggct
    72781 tgtcgtactg gcacgccagc tcgacgatgg cggcgaactg ggtgtcgcgc ttggcgccct
    72841 gccccacatt gccgggattg atgcggaatt tggccagcgc ttcagcgcag gcgggatgat
    72901 gggtgagcag cgtgtggccg ttgtagtgga aatcgcccac cagcggcacg tccagattca
    72961 tctgcaggag ccgctcgcgg atccgcggca cggccttggc ggcctcgtcg ttgttgaccg
    73021 tgatgcgcac cagttcggag cccgcccgcg ccagctgggc gacctgcatg gcggtggcga
    73081 aatcgtccgc cgtgtcggtg ttggtcatgg actgcaccac caccggcgcg tcggatccga
    73141 tgaggacgcg accgacccgg acctgccggg tcggatggcg ggccgccgcg cggccggaaa
    73201 gaagaagatc catcgtgtcc gtactcattc cagcttcagg cgggccaccg tgaccttggt
    73261 ggacgccgcc aggtcgacgg gcttgccgtt gcgttgcagt ttgacgtagc gcgcattgcc
    73321 caccaccagt gtaaagggcg cctggccctc cacaccgagt cggccgcccg cctgtccgag
    73381 cttggacagg accgtcttgc cacgtccgtc cttcacctca acccaggcat cctgttcaaa
    73441 atcgaaatcc agacgctccg acgcaccgtc gcccaccctg gcctccaccg ctggaggtgc
    73501 cttcgcctcg gcggtccgac tttccgtgag aggcgccgcg gagatcaccg caggcgtcgg
    73561 gaccgctgga accggagtcg cagtgccggc cgtgccggcg ggcaggaccg cgggcgcagc
    73621 aaccgccgca gcgccgtcca ccgggaccgg cgcgggaaca cctgccgccg cggactcgcc
    73681 ggcgggcggc acggccggga ccggcggttc gaccggcgtg gcaacagccg gcggtgtctg
    73741 cggcagactc gccatcagtt cctccgcggg cttgcgccgc agggaatcgt agtaccagcc
    73801 caccaccacc acgctgagca aggccaaggc cgcaacgaca ccgggcagca ccgaacgccg
    73861 gaaccgcccc cgcccgacct ggggcatgtc gcccttggca ttggagaccg gcgtcagttc
    73921 catcagccgg gcatcgtcgg gtgcctcgag ggccgccagc aggggctgcg gatcaagatg
    73981 cagcaggcgc gcatagttgc gcacgaagcc ctttgcgaag gcaaaccccg gcagatcctc
    74041 aaagcggccc gcctcgatgg cctcgatctg ccgcggcgtc agcttgagtg tcgccgccac
    74101 ctcggcaatc gacagcccct gacgctcccg ttcgccccgc agggtgacgc cgggcacgga
    74161 ggaagacgcc agggacacgg cgttatcgac ggaatcgttc actgatactg cccttgcaag
    74221 aaagcctgat attcgggtga cgcggggaaa cggcgccgca gttgctggac gagggatgcc
    74281 tcggccgtgc ggtcgcccag gcgatgttcg atgcggatgg ccagccacag gctctcgggt
    74341 gtcggctcgc ctgcttcatg cagatcgacg atgacgcgct tggcgccctc gtagttggcc
    74401 cgccggtact ggatcgccgc gagctggaac aaggcctggc ggttcccgtt gtcggcttcc
    74461 agcgcgcgga tgaactgctg ctccgccgcg ccatcgtcct tgagacgcag atagcagagc
    74521 cccatgttgg tgaaggcgcg ggtcggcgtc tggtaatacg gattgcgggc ggcgcccgcc
    74581 agttgctcga tcccggcctt ttccttaccc atggaacaca ggaaccagcc gtagctgttg
    74641 gcgatttccg ggtccccggg cacaaggcgc agcgcgtgct cgaaattggc cgcggcgacg
    74701 tcgttctctt cgaggaacat atgcaccagc cccatgagct ggaaggtcgg cgcgtagccc
    74761 ggatcataag cggccgccat cttggcttcg tcaagcgcga cgccgtagcg gccggcctcg
    74821 agataggcct gaccgagttc cacatggacg cgcgccttgc gccgggcatc ggtggtgacc
    74881 ggtgcctggg actggggacg ctgggccacg taactgcccg acggcgccgg tccggccggg
    74941 acggcgcacc cccccagcag cgcgaccagc gcaccgacgc cgagcagccg cttcacgggc
    75001 gcacctcggc gatcggcacg acccgtgcgg cggtccgctt cgtcttgtcc tgcacctgac
    75061 cggcgagctg gccacaggct gcgtctacgt cgtcaccccg ggtcttgcgg gtggtggtga
    75121 cgatgccggc gtcgatgagg atctcggcaa aacgccggat acgcggcgcc ggcgaacggt
    75181 cgaagccgga ttgcgggaac ggattgaacg gaatcaggtt gaacttgcag ggaacatccc
    75241 gcgtcagggt gacgagttcg cgggcgtggg cgtcggtgtc gttcaccccg tccagcatca
    75301 cgtattcaaa ggtgatgaag tcccgcggcg ccttgaccag atagcgctga caggcggcca
    75361 tcaattcacg cagggggtat ttctgattga tcggcacgag gcggtcgcgc aaggcgtcgt
    75421 tggacgcgtg cagggacacg gccagggccg tgggacaggc gtcgcgcagg cggtccatgg
    75481 ccggcacgat gccggaggtg gacacggtga cgcgccgccg ggaaagtccg taggcgttgt
    75541 cgtcgagcat caggcgcagg gccgagacga cattgtcgaa attggcaagg ggctcgccca
    75601 tgcccatcat caccacattg ctgatgaccc gctcgccgtc gccggtggca ccgagcagac
    75661 ggttggcgag ccacagctgg ccgatgattt ccgccgcagt gaggttacgg ttgaagccct
    75721 gcttgcccgt cgagcagaag gcgcagtcga gggcgcagcc ggcctgcgac gagatgcaca
    75781 gggtgccgcg attggtttcg gggatgaaca ccgtctccac cgcgttggca ttgcccacgt
    75841 caagcagcca cttgcgggtg ccgtccgtgg agacgctgtc gcgcaccggc accggtgggg
    75901 tgatgcaggc tgtctccgcg agcctggccc gcagggactt ggccacgtcg gtcatctgct
    75961 cgaagtcggt tacgccgaag cgatgcaccc agcggagcac ctgcttggcg cgaaaaggct
    76021 tctcaccctg ctggacgaac cagtcggtga gggcagcggc atcgaaatcg agaagattga
    76081 ccacgggcag ttccattcag tccggcaggc ggcgcaacgc gcacccgccg gaaagagatc
    76141 ctcagcggct gtaaacgttc atgccgggga agaagaaagc cacttccaca gccgcggttt
    76201 cgggcgcgtc ggagccatgc acggcgttgg catcgatgga ctcggcgaag tcggcgcgga
    76261 tggtgcccgg cgcagccttc ttcgggtcgg tggcacccat caattcacgg ttcttggcga
    76321 tggcgccttc accttccagc acctggatca tgacggggcc ggaggtcatg aaggacacca
    76381 gatccttgaa gaagggacgc tccttgtgca cggcgtagaa ctcgccggct tcacgctcgg
    76441 acaggtaggc cagcttggcg gcaatgatct tcaggccggc gccttcaaag cgggcgtaga
    76501 tctggccgat cacgttcttg gcgacggcgt cgggcttgat gatggacagg gtgcgttcga
    76561 tggccatggt acggggctcc agatgagtcg aagaagtcgg taagggttaa aaaaccgaaa
    76621 gcttggaatt ctagcaggta caggtgcaga ttcgaccatt tttcggtcac gcccaaccga
    76681 atgcacacgt gatccatgaa aaacgggggc cgaagccccc gcacctgcgt cctgaagccc
    76741 gcttgccaac gcgccagaac ggcgcgccgg cagccactca gaactggtcc tcttccagcg
    76801 ccagcgcgcc ggccgcgcca ttcaccaccg cgtaagccag accaggcgcc tgggacagca
    76861 cgtggtcggc gtagaagcgg gccgtgacga tcttggtctt gtagaaggtg gcatcaccgc
    76921 tgccttccgc gaggcgccgg gccgacacga gggccgcgcg ccccatctgc cagccgccgg
    76981 tgacgatgcc cagaagcttg aggaagggca cggaaccgac ggaggcggcc ttgatgtcct
    77041 gaccgaaggt ggcgaggata taggcgaccg cttcttccac cgcggtgatg ccggcggaca
    77101 gggacttgcg gatggccgcg aactcatcgc cggcctgctc ggcgagctgg gcttcgacct
    77161 gacgcatctc ggccaccacc gccttgaccg tctgtccgcc ttccttggcg atcttgcggc
    77221 cgatcaggtc gttggcctgg atggccgtgg tgccttcata gatcggcgtg atgcgggcgt
    77281 cgcggaagtg ctgggccgcg ccggtctcct cgatgaagcc catgccgcca tgcacctgca
    77341 cacccaacga ggtgagctcg acagagtttt cggtcagcca gcccttgacc accggaatca
    77401 gcagatcgac gaaggcctgg gcgcgcgccc gctcggcgtc atccgcgtgg taatgggcct
    77461 tgtcggtggc cgccccaacc acgtaggcca gcgcccgcat cgcctcggtg gtggatttga
    77521 tgaccatcag catgcgccgc acatccgcgt ggtgcaggat gttgaccttg gggcccccgc
    77581 gcacgccggc gtcggtgccc tggatgcggt ccttggcgta gcccagcgcc tgctggtagg
    77641 cccgctcgga cagggccaga ccttccattc ccaccgcgaa gcgcgcctcg ttcatcatga
    77701 tgaacatgta ctcgagacca cggttggcct cacccaccag ggtcccgatg gcaccgccgt
    77761 tgtcgccgaa ggccagcacc gccgtggggc tgccatggat gcccagcttg tgctcgatgg
    77821 acacgcagtg cacatcgtta cgggtgccgg gcgttccatc ggcgttgagc aggaacttgg
    77881 gcaccacgaa cagggaaatg cccttcaccc cttccggcgc gtccggcagg cgggccagca
    77941 ccaggtggat gatgttgtcg gtcatgtcgt gctcgccgta ggtgatgaag atcttctgcc
    78001 cgaagatctt gtaagtacca tcaccctggg gcacggcgcg ggtacgcacg gcggccagat
    78061 cggaaccggc ctggggctcg gtcaggttca tggtgccggt ccattcgccg ctcaccatct
    78121 tgggcaggta ggccgccttc tgctcgtccg taccgcgcag cttgatggac tcgatggcgc
    78181 cattggtcag catcgggcac agggagaagg ccatgttggc cgatttccac atttccatca
    78241 ccgccgtgga caccagcttg ggcagacctt gtccgccgaa ttccggctcg caggccaggg
    78301 ccgtccagcc ggcttcggca aactgcgtgt aggcgtcctt ccagcccggc gaggtatgca
    78361 ccacgccgtt gtcccagcgc gggccctggg tatcaccaat gcggttgagg ggcgccagca
    78421 cgccgccggc aaacttgccc gcttcctcca ggatcgcatc caccagatcg ggggtggctt
    78481 cctcacaccc cggcagtgcg gccacttcat ccagaccggc cagctccttc atgacgaact
    78541 gcatgtcgcg gatcggtgcg ttgtaggtgc tcatgtgtct ttctcctcga aatcttgttt
    78601 gttttgagtg gatcggcccg ggctgaaagg gcctatttgt tcatcagata actgcgatca
    78661 tcgaaactcg ccacccactc cgggcgatag atcaccagga gggtcagcgc catccccgac
    78721 agccacgcct cggaaaagcc cagcagcagg aagaagggaa ggtattccgt caacagcaca
    78781 tccgcctcgt aggccccggc caggaacagg gatgcggagg ctagggtgcc ctgcgccagc
    78841 accacgaggg ccgcacagag aaaggcgttg acgaagatgt aaatgaagaa atgcttcggc
    78901 agcacgcgct cgaccaggcg aaacgccagc gtcgcgagcg cgaccgggat gatcaccatc
    78961 agcagcagat tgatcggata cgccgaccag gcaatgctgc cgttcagggt gatggcaccc
    79021 agcgtcagcg ccaagaccag aagcgccagt tgcggaccga agatcagggt caccgccatc
    79081 ccgcccacga gatggagatt gagccccggc ctgacacccg cattgatgct ccaaagcagc
    79141 gccaggatca ccgtggcgcc cagcaccgca ttgagctgat gcccatccag caggcgcttc
    79201 cagggcccgg tacgcacgac ccacagcccg acccccaatc cacccaggat ggcaacgacg
    79261 tgccatccaa gcggaaagag gtcggctgca agattcatga cgcccgcggg gcccggatca
    79321 gagggacgag gtcagctggg gcaccgcttc gaacagatcg gccacgaggc cgtagtcggc
    79381 cacctgg
//
LOCUS       DAOQMM010000005        75163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_45_length_75163_cov_48.188302, whole genome shotgun sequence.
ACCESSION   DAOQMM010000005 DAOQMM010000000
VERSION     DAOQMM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 75163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75163
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45_length_75163_cov_48.188302"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..400)
                     /locus_tag="PLH85_01540"
     CDS             complement(92..400)
                     /locus_tag="PLH85_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63267.1"
                     /translation="MTTKQKGKCPFCEKVVAPVIVEENTIRRDRCKCPECGEEIYLCR
                     SPGCHDFAKGTSVYDHELCPSCTETVSNLASEFGKAALKVGVAVATAVAVAAAKNNEK
                     "
     gene            complement(932..1303)
                     /locus_tag="PLH85_01545"
     CDS             complement(932..1303)
                     /locus_tag="PLH85_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63268.1"
                     /translation="MTAAGKNRLPPQRVQDDIDAYLAIKVIPDWNPIKPEYAVDAITR
                     AHDALRAAQEAELHAQHTLDAARDALVSQQWDFHSIILGAKDQARAMFGPDSDHVAAL
                     GLKKKSDKKSPSRGPKPKPTE"
     gene            2090..3247
                     /gene="dinB"
                     /locus_tag="PLH85_01550"
     CDS             2090..3247
                     /gene="dinB"
                     /locus_tag="PLH85_01550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNB63269.1"
                     /translation="MPPLPRSIAHLDMDAFYASVELLRAPELRGLPVVVGGRRALTAA
                     AGETHPRLRDYVGRGVITTATYEARALGVHSGMGLMKAAQLAPDAVLLPADFEAYRHY
                     SQRFKQAVRAIAPEVEDRGIDEIYLDLSALNPDGSVAGARALALRLQQAVRAATGLSC
                     SLGVAPNKLLAKLCSDLEKPAGITVVTLDDLPTRIGPLPARRINGIGPKAAARLEQLG
                     IDTIAQLAAADLDLLQHHFGERYGLWLQRAARGEDERPVVTRSEPKSLSRETTFERDL
                     HPRSDRQELSARFTRLCEQLAADLARKGYVGKTIGLKLRFDNFHTVTRDHTLAEPVGD
                     APTLRRVAGECARRVPLERRIRLLGVRVSGLVRRDALPPAESRPAQAELFA"
     gene            3287..5680
                     /gene="ovoA"
                     /locus_tag="PLH85_01555"
     CDS             3287..5680
                     /gene="ovoA"
                     /locus_tag="PLH85_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNB63270.1"
                     /translation="MPRPLPDAADEPWRQNLRGEHDALLQGARPDWWWTGLPPTDCPG
                     RQPDGRLTALPAPNLATCTRDEVLAVFDNGWTLTEVLFAGLQGEEAFYRPPYHHLRHP
                     MIFYYCHPAALYINKLRVAGLIDAPLNPYFERVFETGVDEMRWDDMSKNAMLWPSFAE
                     AHSYRAEVYATVRRVIETHPDLATGHAPLGMDHPLWALFMGFDHERIHLETSSVLIRE
                     LPIALVQPPDVWPAVHPSAAAAATAEFPPRAGADYPVARWVDVPAASVRLGKPRDWPS
                     FGWDNEYGERRVAVQGFRVEDQLVSNGAFLEFIQAGGYLEPRWWSEAGWAWRSFRNSK
                     WPCFWVPDGPAGLHRYRLRTVFTIVPMPWAWPAEVNAHEAAAYCAWRSERDGHPCRLP
                     TEAEHNALRAPAWAAEDPAFRQDGAALGRDTGWNLQLAWGSPWPVDAGTPTAAGVRDV
                     FGNVWQWHGDDFNPLPGARVHPYYDDFSTPCYDGQHQMLLGGSWVACGDEASVWARFH
                     FRPHFFQHAGFRVVQAEHDGGVVRLGAADSGAQVYEDARMVDDYMVLHHARPEAQMPW
                     PAGPRGATGFPQRCADWLLKGAAQHASGTARALDIGCAVGGASFALARGFDAVLGVDL
                     SRAFIDAASTLQRDGRRDYVLRDEGELGDTLSAVIDPTLERSRVSFRQADACSLPAEL
                     ADFDAVLLANLLCRLPSPKSLLGRLGGPRGLVKVGGLVALFSPYSWLEQFTPQEAWLG
                     GFTADGRAVRSGDALKAFLTEEGFVLRREEDVPLVIREHARKYQYIVTHGMLWQRVR"
     gene            5761..6339
                     /locus_tag="PLH85_01560"
     CDS             5761..6339
                     /locus_tag="PLH85_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNB63271.1"
                     /translation="MSTLIQPARRGFLSTSGKTALSVAALGLLAGRSDLASAASGNAA
                     GDVAILNVALGLEHEAINAYQLGAGSGLLQKPVLDVAVLFQSHHKGHRDALIATIEKL
                     GGTPVKEKSLDEYARALKADTLKNQADVLSLAARLELGATNAYLGVIPSFKDAQLAQV
                     AGRLAADETMHWTALSSALGRSLPGGALSFGA"
     gene            6347..6730
                     /locus_tag="PLH85_01565"
     CDS             6347..6730
                     /locus_tag="PLH85_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB63272.1"
                     /translation="MGARHFALVAGLALIGPATAQDAARGAQLYEARCGGCHSPEANR
                     IGPRHAGVLGRRAGSLPDFAYSPALARSTVIWRNDTLERWLADPEALIPGQRMNYRVA
                     DAQDRRDLVAYLASLQVVAPDGGRR"
     gene            complement(6967..8718)
                     /gene="arsA"
                     /locus_tag="PLH85_01570"
     CDS             complement(6967..8718)
                     /gene="arsA"
                     /locus_tag="PLH85_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HNB63273.1"
                     /translation="MALPDSRTRYLFFTGKGGVGKTSLSCATGLALADAGKKVLIVST
                     DPASNLDEVLGAALGPLPTAIPGAPGLFALNIDPEAAARAYRERMVAPYRGILPAAAI
                     RSMEEQFSGACTVEIAAFDEFSKLLGDAAATTDFDHVIFDTAPTGHTLRLLTLPSAWS
                     GFIASSTGGASCLGPLAGLEQQKALYAATVARLSSAADTTVVLVSRAEAASLREAERT
                     RHELAGLGVSNLLLALNGVFRTERQDDAVATALSRRAAIALADMPAGLAALPATTIPF
                     LPRGTVGLAALRQMAHPESVAAPTVPDAAQTALPPGLAGLVETLAAAGHGVIMTMGKG
                     GVGKTTVAAAVAVALARRGHPVILSTTDPAAHVASTLDGQVPGLSVSRIDPAEEVARY
                     TREVLDKAGAQLDAGGRALLEEDLRSPCTEEIAVFRAFARTVEAGREGFVVLDTAPTG
                     HTLLLLDAAEAYHREVMRTQGDMPESVRELLPRLRDPDYTHVLIVTLAEATPVHEAER
                     LQHDLGRAGIAPFAWVINQSLLASGTRDPLLSQRGAWEIPFIRRVADELAPRCALIPW
                     LAEAPVGEAGLAQLLRT"
     gene            complement(8729..9094)
                     /gene="arsD"
                     /locus_tag="PLH85_01575"
     CDS             complement(8729..9094)
                     /gene="arsD"
                     /locus_tag="PLH85_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HNB63274.1"
                     /translation="MKKLEVFDPAMCCSTGVCGVDVDPVLAQFAADLQWVEAHGIAVE
                     RHNLGQAPQAFAANPAVLKEMEAGMDRLPILVVDGHIVATGMYPSRVQLAQKLGIALT
                     TADKPHIKPGGCGCKPGSC"
     gene            complement(9105..10181)
                     /gene="arsB"
                     /locus_tag="PLH85_01580"
     CDS             complement(9105..10181)
                     /gene="arsB"
                     /locus_tag="PLH85_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNB63275.1"
                     /translation="MSVIQCEVGAKAAAGAPMSVFERYLSVWVGLCIVAGIVAGQLAP
                     GLFRTIGRLEVAQVNLPVGLLIWVMIIPMLVKVDFGALHEVRQHVRGIGVTLFVNWFV
                     KPFSMAFLGWLFVRQLFAPLLPAEQLDSYIAGLILLAAAPCTAMVFVWSRLSDGDPLF
                     TLSQVALNDTIMVFAFAPLVAFLLGLSSITVPWDTLLTSVVLYIVIPVILAQLWRRTL
                     LARGPAAFDAAMAKIGPWSIAALLATLVLLFAFQGEAILRQPLVIALLAVPILIQVFF
                     NSALAYGLNRLVGERHSVACPSALIGASNFFELAVAAAISLFGFESGAALATVVGVLI
                     EVPVMLLVVKVVNASKGWYEAHHT"
     gene            complement(10191..10604)
                     /locus_tag="PLH85_01585"
     CDS             complement(10191..10604)
                     /locus_tag="PLH85_01585"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNB63276.1"
                     /translation="MNLLKTVLVLCTGNSCRSQMGEAILNYELAGVVRALSAGTAPQP
                     KVADGALEALRLAGLPTEGLHPKTVEAVMDQDIDLVVSVCDNAKESCPIFPRPVMRIH
                     VGFHDPHGEPLDSFIAVREDIRARLVPAVRLALGL"
     gene            complement(10665..11078)
                     /locus_tag="PLH85_01590"
     CDS             complement(10665..11078)
                     /locus_tag="PLH85_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB63277.1"
                     /translation="MDAVAGRIDGLEYFHIIRNMELLDAAEVLAALGHESRLAIFRLL
                     VEAGPAGLNVSAIAERMGGVPGATLSFHLSHLSRVGLIKGERQSRFIHYSAEYATMDE
                     LIAFLTRNCCEGKACLPKTAQCDTTAKRRAAPADT"
     gene            complement(11141..11569)
                     /locus_tag="PLH85_01595"
     CDS             complement(11141..11569)
                     /locus_tag="PLH85_01595"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB63278.1"
                     /translation="MTCFSTSRHFARTPAEVFAAVRDPERLARWWGPDGFTNRFERFE
                     FQPGGCWYFTMIGPDGTAYPNQAEFTRIEPECCVVIRHTCAPHFTLTLTFEPEAGGTR
                     LHWAQAFDDPAVAEALRAIVVPANEQNLDRLAAVLGLTPA"
     gene            complement(11601..14519)
                     /locus_tag="PLH85_01600"
     CDS             complement(11601..14519)
                     /locus_tag="PLH85_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB63279.1"
                     /translation="MTSQDEAKPRILIVDDINDNLHTLMNILRGEFAVVVATNGPKAL
                     ELAVRTPPPDLVLLDIRMPGMDGYEVLHRLKSDPGTADIPVIFVTGLSDAADEAVGLR
                     LGAADYVTKPINPELLLVRVRNQLELRTHRRKKAVEGLGAAVMARSDKPALLLVDDVP
                     ENLHGLVAALQDDYRIMVAPTGAKALELVAGVNPPDLILLDILMPQMDGYEVCRRIKA
                     TPVGNRIPVIFVTVVDSVVDKVQGFSIGAADYITKPFDIDEVRARVRTHLDLSRLQRS
                     LEKLVVDRMGALQEANDALAESKAKYQILAEYSPNWEYWVAPTGGYLYVSPACRDVSG
                     YGAVDFIADPDLMRKMIHPDDLYIWDAHNERSPESGGHRLPNFRIAARDGSERWIEHV
                     CQPVIDDRGRFLGTRGTNRDVTQQVAAEEQLHLAATVFANTSEAVMITDADNRVLTVN
                     PAFVEITGYSQDDTAGREPLVLRADVMEVDAHRAMWAAIHATGRWQGEINGHCRDGRD
                     FIGWVTVSTVFKDDGSVNRRVVLFSDITEKKRADNLIWTQANYDHLTRLPNRRLFNDR
                     MEREIKKAQRKASVIGLLFIDLDRFKEVNDTQGHEVGDLLLVEAASRIRHCVRDYDTV
                     ARLGGDEFTVILSDITEGTDIGRVARSIIDRLSEAFVLNAQEHFVSASIGVALYPRDG
                     ATISELIKHADQAMYLAKDAGRSRFRFFTRALQESAEHRMRLAGDLRRALEAGQLEVY
                     YQPIVDLDTGRIAKAEALLRWRHPAHGFVSPATFIPIAEDTGTIHEIGEWVFAQVIQQ
                     ASRWRERLAPEFQVSLNMSPVQFNGRNPTQWLELLQTTQLPGSNLVIEITEGLLMSSD
                     PGIKETRLGLRDLGVGVAIDDFGTGYSSLAYLKKFDIDVLKIDQSFTRNLDPNSADFA
                     LCEAIVVMAHKLGLKVIAEGVETDQQRNLLRQIGCDHAQGYLFSRAVPAGQFEALCAR
                     "
     gene            complement(14516..17950)
                     /locus_tag="PLH85_01605"
     CDS             complement(14516..17950)
                     /locus_tag="PLH85_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB63280.1"
                     /translation="MRALFEGMQIKAKLVLIIVITSVAALLIEAVGFIAYERVRVTDE
                     LVRDLSSLARIVADRSSAALLFYDDKVAMETLGALRIKRAVSAACIYDVGGRVFARYE
                     RGEDRPFSFPADPKFESRSYTEGGYLHLIEPILVEGTPQGAVFIRASFRELDLLWRDY
                     LLFAALTAFVTSMITLLVATRWQRIVSRPIERLTRTVQMVAISKDYSVRATLDSDDEI
                     GTLVEVFNGMLAAIEDRDTVLLDANRRLSENEAQLKSVNEALEQRVRERTAELQALSD
                     SASVGIVLLKDGLIVRSNRRLDEMFGYAPGEQTGRPLRDRLADTVDEEAEALAAARLA
                     AGDTVRRELMARHKDGSAFWIRLSARVIDRADPARGAVCVIEDITAERDAMDEMIKAK
                     TMAEEASRMKSDFLATMSHEIRTPMNAIIGMLYLALKNEPPAALRNYLAKAQGAAHSL
                     LGIINDILDFSKIEAGKLEIESIEFGLDTVLEQLKDSVGLQAEQKGLEFLIRYDVNIP
                     PALLGDPLRLGQIMLNLCSNAIKFTEQGEVELGFRCVAMTETDLELQFSVRDTGIGIA
                     PEVAERLFQKFTQADQTTTRRFGGTGLGLAISRRLAELMGGRLWIEQSAPGKGTTVCC
                     SIHLQIARDAQTHRRRLVEQAGPLLDGVRVLVVDDNEVSREIMAEMLRFFHLDVSVAG
                     SGAEALERLQVPDAAPVDLVLMDWRMPPMNGDEVTRRIHASPLIRHKPKVVMVTAYGR
                     EDVIKSAEQAGVAGFLVKPVSPSTLLDTILSNLGRGRILGKESSRSERGGACDFRGAR
                     LLLVEDNDINREFATELLRSMNTEVECAVNGEEAVAMVQRAPYAAVLMDIHMPVMDGL
                     EACRRIRALGQQPGGEHLGELPIIAMTALAMAHDEQRSLAAGMNDHITKPVSPERLRA
                     ALSRWLNIPLGEGGAPGPVPSSAPGSDLRALRSLDAEQGIRRIGGDVEAYRKQLRRFR
                     QRYDTAAERLRDLVAHHSPRQAEEYCHALKGVCGNIGADALFACVSDIDNLLKQEIRP
                     DEASLARMAELLATVMADIDSLAAPEQAGAPAGGGLADTDVLARVGRLRGLLESDVGA
                     AQSVLAELLAGIAGSPLEAAVREVAERVDVFEIDEALGLLQALQTQLEGRTGATPP"
     gene            complement(18042..18590)
                     /locus_tag="PLH85_01610"
     CDS             complement(18042..18590)
                     /locus_tag="PLH85_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB63281.1"
                     /translation="MRASVRRRFRLVPLLRRLLVCAAALPLMAFSAPGELEAKIKAAY
                     VFHVIKFVDWPVLPTDALRLCVSGSDPVGVLLGELASKQVKDRPLRVDIDNGFDPAQC
                     QVLFVGRGERRWADLQARLRGGSVLTISDQDGFARIGGVVGFYSDGGRIRLEINPDAA
                     RNANLRISAKLLEVARTVPQDR"
     gene            complement(18610..20658)
                     /locus_tag="PLH85_01615"
     CDS             complement(18610..20658)
                     /locus_tag="PLH85_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB63282.1"
                     /translation="MKKAYLASTFVLISAPSLADAPADPGEIRNLPLSGFVDQLSLEQ
                     LGNLVVTDTKLAQAADTVTQRIVVLRNEDIERQPAHSGNLAELMRPTSGQFVNVLSRN
                     DANWGSYAGLGPKYNSYLLDGLPIDSFVDAMSLDPSAIERVEVHKGPASVLYSNYLTM
                     DFVGNETPLAGTTNFVLKSRVDTPLTRVAAGAGSWGTVFGKAYAQGTQGNLGYIVSAN
                     LEHSDYTQYGTRNSWLQTTQAPAYQKNRFFGNFSYAFDRPDHTVTVFYQRTLHQGTMG
                     RPNRGFDHTYDTLNFAYSNTFSDDWNIQFKYGERRYDRRFANDNYPTDLSLSNHEFTR
                     QTIRPADLTLSHRHWGESLLTAGVDGQWVDYQTDVQDPVGAVTPSNRAQARSTGYFVQ
                     EKLVWDAWVLRGGVRHDTIHHDYDLLGGITPATRSAAWSKNLWSFGTRYNADPGLAFY
                     GNVGTSFMAPAAKQIGGTVAVPTASGELPNPGLKPETGVGRDVGVNWRASQALTLDLR
                     GFLNTINDAIVTNIVSAAPSQVRSENAGKARAMGYEVDLRYAASSETSVFGNLTRTHT
                     RVTDPTNADHDGTAIPFAPDTLANAGVLASLPGDMSVATYYHWVGRYYDSTSRSARLG
                     FGQYGVVNVRVQKPLYRDARAAVHLTVDLNNLTDRHHAMPFDFRDPGFNGFAGVDVRY
                     "
     gene            complement(20796..21716)
                     /locus_tag="PLH85_01620"
     CDS             complement(20796..21716)
                     /locus_tag="PLH85_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB63283.1"
                     /translation="MDKLKAIGTFVRIADEGSLSGAARVLDMSLPAVVRALAALEADL
                     GVRLFNRSTRRIALTEEGRRYLDSCRNLLAALDEAESGLRDEGGEPSGALTVTAPVLF
                     GQLFVAPAITRFVQRFSRVHCRVLLFDRIVNLLEEGVDVGIRIGVLEDSTLVAHPVGH
                     VRRMVVASPDFLARQGVPAHPRDLLRANCIGFSPTPGVWWRFEEQGRSFGVPVRGNLE
                     FNHVAPALDACIAGLGFGMFISYQVAPHLAAGRLVPVLEAFEQAPRPVSVVVPHARLL
                     PLRVRAFVDWVRADLSAHLRDAPGTPGSTA"
     gene            21824..22456
                     /locus_tag="PLH85_01625"
     CDS             21824..22456
                     /locus_tag="PLH85_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNB63284.1"
                     /translation="MNTPAQPIRFHRFATSGHSHRAELMLSLLGLPVEPIDVDLAGGA
                     QKTPAYLAMNCFGQVPVIQDGDVTVADSNAILVYLASVYDDGRWLPRDPAAAAQVQRW
                     LSVAAGQLAYGPCAARLVTVFGARFNADEVIARAHALFKILDAELATRAYLTGDAPTI
                     ADVALYSYTAHAPEGNVSLDPYPHIRAWLARIEALPGFVPMPATAAGLRA"
     gene            22463..23425
                     /locus_tag="PLH85_01630"
     CDS             22463..23425
                     /locus_tag="PLH85_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNB63285.1"
                     /translation="MTAQTPPRSVFHAGEEALQALAGAQARLAETGPRVIREVLPEQH
                     RSFFAELPFAVLGALDADGQPWATAIAGPPGFITSPTPERLRIGALPAADDPAAAGLA
                     PGAAIGLLGLQPHTRRRNRANGRVAALDADGFTLAVEQSFGNCPKYIHARRVEHAAQT
                     AAPAPARVQARLDGATADLIRHADTFFIATAHPDAARGEHAAHGVDVSHRGGRPGFVG
                     VDGDHLWVPDFAGNQYFNTLGNLLLNPRAGLLFVNFSEGSLVWIAATAQILHDGRAVD
                     AYPGAQRVLQLTVHALWTARRRLPLHSQDGEASPFLAGTGIWPG"
     gene            23497..>24230
                     /locus_tag="PLH85_01635"
     CDS             23497..>24230
                     /locus_tag="PLH85_01635"
                     /inference="COORDINATES: protein motif:HMM:NF020509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB63286.1"
                     /translation="MPVHHDAFISYSHTADGALAPAFQRGLEQLAKPLLRLRAQKIFR
                     DQTDLTASPALWPGIVSHLAASDWLLVFANPLYAGSRWCRKEVEWWLAERSPERLLIV
                     LTDGDIVWDEARRDFDWARTTALPDCLAGHLADEPLYVDLRWARETPGLSLAHGGFRE
                     AVVRVAAPLRGVSPTDLDGADLRQLRRTRLFVRTGVGAICVAAGVAVWQARVATEEAR
                     IAETQRQQAEAQRRIAESERAQAEAQR"
     assembly_gap    24231..24330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24331..25146
                     /locus_tag="PLH85_01640"
     CDS             <24331..25146
                     /locus_tag="PLH85_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63287.1"
                     /translation="AQRHIAESERARAEAALAASERELLRAQSAELRALRARLDTLIA
                     DAITPGHLTEIAPMQAERALLDQRLADVTRQYLARLAEQIGYRGGFEFLMKWEGNAGR
                     VALIGSSVFIDPLTDLALAKPDDLRRRYEFLLTPAELQALIATAGKRGAEAQAAYAAA
                     PILARIRIRAEDVARLVPDVIEPFWKRLRDKHPILRRPDTPPAVHTALLSVAVNLGAR
                     VESLAPFIDAGRWRELADAIDALAARPPVLAGLKRRRAEEAALIRAALAGQPP"
     gene            complement(25153..25290)
                     /locus_tag="PLH85_01645"
     CDS             complement(25153..25290)
                     /locus_tag="PLH85_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63288.1"
                     /translation="MTTLRIVHRDARANALDHPARFFAGCSLVVLGAGVWCAQAVSHL
                     F"
     gene            complement(25425..26159)
                     /locus_tag="PLH85_01650"
     CDS             complement(25425..26159)
                     /locus_tag="PLH85_01650"
                     /inference="COORDINATES: protein motif:HMM:NF010071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HNB63289.1"
                     /translation="MKPRASAGPRTLTLRILRCGPDIDPHWQDFRIEETEGMTLFIAL
                     TEVRAHQDPSLQFDFVCRAGICGSCAMLINGRPGLACRSLTRDLDPLITLAPLPVFEL
                     IGDLSVNTGKWMRGTAERLETWIHTTAEADLTAVEAPMEPALAEAIYELDRCIECGCC
                     VAACGTLRMREDFVGAVGLNKIARFRLDPRDERSDDDFYELIGDDTGVFGCMSLLGCH
                     DVCPKGLSLQTQIAFLRRKMAVTGLK"
     gene            complement(26156..26479)
                     /locus_tag="PLH85_01655"
     CDS             complement(26156..26479)
                     /locus_tag="PLH85_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB63290.1"
                     /translation="MNAPTPTRIVTLRLADHPDEYALLDTEGLTLLNALNHIRPSLPA
                     PLGDAFVCHAGACATCYVLVNGERCLPCTTFVRDLPDEFTVAPGVHSAGWDEQRHDVR
                     RGHGQ"
     gene            complement(26476..>27996)
                     /locus_tag="PLH85_01660"
     CDS             complement(26476..>27996)
                     /locus_tag="PLH85_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HNB63291.1"
                     /translation="DGTGHAMLYAVGDQAVAAGIPVHERTEALALIHEGGRCHGAIVR
                     DLVTGALSAYVAKATCIASGGFGRIYQVSSNAIINEGIGAAIALETGVATLANMEAVQ
                     FHPTTIFPAGILVTEGCRGDGGLLRDAAGHRFMPDYEPEKKELASRDVVSRRMEEHIR
                     AGHGVKTRYGDHLWLDITLLGAAHIDGRLREVKEICQHFLGIDPSRDWIPVRPAQHYS
                     MGGIRTDASGHVRGLRGLFACGEAACWDLHGFNRLGGNSVAETVVAGMIVGERMADFV
                     ESPMGELQVSTALVRDCLERTQHHLDTLLSGTGTEDAQALLARMQAIMTEQVGIFRRG
                     DLLESAVAELASLLERSRRIGIATRRPGANPELVTAYRVQKMLKLALCVADGALQRTE
                     SRGAHFREDFPRRDDALWLKRTLATWPDPQQTRPTLSYEALDVMRMELPPGWRGYGAR
                     NAVEHPDTARRADEIATIRSAFATADRYTVQQALMPYEHLVPTRFRGRNHRSGEHT"
     assembly_gap    27997..28096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28260..29117)
                     /locus_tag="PLH85_01665"
     CDS             complement(28260..29117)
                     /locus_tag="PLH85_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HNB63292.1"
                     /translation="MAPTAVRTADAESLSAAPRVVAPTDRGSVRSRASRWPARLDVLQ
                     SVSGLFLAVFLIAHMFFVSSILISKDAFYTVARFFEGAWLLGRPQPWLVSCVVGFVLL
                     VFVGHAWLALRKFPANFRQYRAFAAHRAGLRHDDTGLWWIQLWTGFALFFLGTVHLYG
                     MLTQPELIGPYESADRIWTGTLWPLYLLLLFAVEIHGSIGLYRLAVKWGWLEGRDAAV
                     SRRRLRVAKTVFSAFFLILGLITLRAYVQIGVTHADRAGERYVPGAHATLVPNPSALA
                     AAPNPPSME"
     gene            complement(29426..30376)
                     /locus_tag="PLH85_01670"
     CDS             complement(29426..30376)
                     /locus_tag="PLH85_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB63293.1"
                     /translation="MSHVHGPGCRHGHHHGHAHGAHGQGPAHHDRAFAVGIVLNVLFV
                     AVEAWFGWRADSLALLADAGHNLSDVAGLALAWGAAFAARLRPTHVRTYGWQRASILA
                     ALANAILLLVAMGALALEAVQRLGAPHPGDAVTVMAVAALGVLINGLSAALLMKGSQD
                     DLNIRGAFLHMAADALVSLGVVGAGALYLAFGWAWLDPLVSLAIAALIVAGTWGLLRQ
                     SLHLAFDGVPEGVDLVEVHAWLRSQPGVRSVHDLHVWALGTSDVALTAHLVMPAGHPG
                     DAFLIALADGLVHRFRIGHPTIQIEIDGLPDGCVRPARLE"
     gene            complement(30373..31449)
                     /locus_tag="PLH85_01675"
     CDS             complement(30373..31449)
                     /locus_tag="PLH85_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNB63294.1"
                     /translation="MIPRDTRLQILDLARWAPSGDNTQPWRFEIAGDDRLVVHGHDTR
                     TWCLYDYNGHASHMAHGALLETLRIAATAHGLEAVWRLRADSTDTAPVYDVTLSPVSG
                     LAADPLLSCIERRTVQRRAMRATPLDSGQRTALAAAPGAGYTVQCFETGAERRAVAGL
                     LWRNAYLRLTCPEAYPVHKDVIEWRARFSKDRIPEQAVGVDAATARLMEWVMASWGRV
                     QFFNRFLGGTIAPRVQLDLIPALACSAHLLLKADVPPRGLADHVRAGAAMQRLWLTAT
                     AVGLHLQPEMTPVIFRWYARSGQRISHDPAIDRGAAALAERFEQIAAIGPDQALSFFC
                     RVGVSDPPRSRSLRKGLDELLLKP"
     gene            complement(31554..32420)
                     /locus_tag="PLH85_01680"
     CDS             complement(31554..32420)
                     /locus_tag="PLH85_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNB63295.1"
                     /translation="MVQFVYDEAFSRNIGWVTVAEQAALRGKRVAIAGMGGVGGVHLL
                     TLARLGIGAFHIADMDVFELVNFNRQVGATVSNLQRPKAAALAEMARDINPELDIRLF
                     ERGVDDANLGDFLDGVDLYVDGLDFFAFDARRATFRACAARGIPAVTAAPLGMGTAVL
                     SFLPGQMSFEDYFCLDGCDEEEMAIRFLLGLSPAMLQRGYLADPSRVNLAEQRGPSTI
                     AACQLCAGVTAVESLKILLGRGDVLCAPRGFQFDAYRNRYVKTWRPGGNRNPLQRLAL
                     FIARRQLRAMRR"
     gene            32607..33821
                     /locus_tag="PLH85_01685"
     CDS             32607..33821
                     /locus_tag="PLH85_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63296.1"
                     /translation="MPFTLAIRNILRQKFRTGMTLAAIIFGVVGLILAGGFVEDIFIQ
                     LGETIIHSQTGHIQVFKKEFLEKGIRYPERYLIDHPEDIARHFDGHPEVEAVMSRVYF
                     SGLLNNGRRDLPIIGEGVEPDKETKLGSLLKITEGQPLTDKDPDGILIGQGVAQSLAV
                     KPGDALTLVTSSAGGAMNTHDFRVVGIFQSFSKEFDARAVRIPLNAGQDLLQTRGVNQ
                     VVVALHRTDDTDAVLKRASAELADSPYDVRSWRTLSDFYDKSVQLYDTQFGVLRLIIL
                     LMVLLSVTNSVNMTVYERQGEFGTMQALGNRARDVVRLVVLESALVGLGGALAGVVLG
                     VIAALIISAIGIPMPPPPNANVGYTAFIRVVPGTLAGAFLVGFVATVLASLFPAYKVS
                     RLPVVDALRQNV"
     gene            33847..34629
                     /locus_tag="PLH85_01690"
     CDS             33847..34629
                     /locus_tag="PLH85_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNB63297.1"
                     /translation="MAFFDNLTLNLGDGFRKYFQISAAFDNAGRDDVFAIRHEVYCEE
                     LGFEPVRANRRETDEYDRHSRHCLLRTSTEPHTLVGCNRLVLARPEDPAYPLPFERTC
                     AATLDRRIIDPAKEPRGAIAEVSRLAVRGLYRRRKGEARSEVALSDSDFGTAAQPRFP
                     YIPISLYLAVVAIAQREGVETLFVLTEPRLADHFSKLGVKIRQIGGPVEHRGTRIPSM
                     MDVKGIIGGLRFFMKPMWRVVNEQIDAGYTSGSPDLPAGSAC"
     gene            34733..35305
                     /locus_tag="PLH85_01695"
     CDS             34733..35305
                     /locus_tag="PLH85_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63298.1"
                     /translation="MYLSPDEIHQNRLDTLHAIEAASQAWIDATGKLSELILRAGRQM
                     LDESERHLSQLAQPGASLPLSIGSLDAWRSDSAELVRDSFAIIAQAHQTTLQIARKQV
                     GIFDLALKRGMAHAIQHADAETGSAISHVRTALEQAETCLNELTDSATRGVGAMEEQV
                     RQIAEALASTADEAPPAAPAPRRTRKPRAQ"
     gene            35415..36236
                     /locus_tag="PLH85_01700"
     CDS             35415..36236
                     /locus_tag="PLH85_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNB63299.1"
                     /translation="MNARSALYQARYGNPDVAVPAAASPVIDHLLAHASVRAYLPDPV
                     SDDTLAAIVAAAQSAASSSNLHAWSVVAVRDAARKVRLADYAGDQAHIRQAPLLLVWL
                     ADLARLEQVARRVGETSAALDYLEMFEVAVIDAALAAQNAVAAAESLGLGTVYIGAMR
                     NRPEDVAAELQLPPRVVAVFGLCVGMPDPAAPAAVKPRPPQSVVLHQETYSLPAQEAG
                     LETYDQAMAAFYAAQGMKVRGTWSNHSAKRIRDAASLTGRDRLAEALRNLGFPLR"
     gene            complement(36304..36957)
                     /locus_tag="PLH85_01705"
     CDS             complement(36304..36957)
                     /locus_tag="PLH85_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63300.1"
                     /translation="MKTSPLDYVLSTIAITLILLLAVAGVWALRPLTTAALADYHVVA
                     DFLLLLLFYGLLSAVAVRVLLRVQPIAPGEYDGDSPVFTFWKLITIVYRLGQGALLPF
                     TTVFTRPVVEALYGARVGADVAIGGTIDDPYQVEIGPRAVIGHNALVSGNVLYGGKLT
                     CGKVIIGAGATIGVNSVVLPGSEIGENAVLMGGSYLMPGTKVPAGETWRGNPARKWM"
     gene            complement(36974..38212)
                     /gene="argJ"
                     /locus_tag="PLH85_01710"
     CDS             complement(36974..38212)
                     /gene="argJ"
                     /locus_tag="PLH85_01710"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNB63301.1"
                     /translation="MPVLLSTPDPVCLLPVPGVRLGVAEAGIRKANRRDLTLIELAPG
                     SRVAGVFTTNRFCAAPVQVCKAHLPAGTIRALVINTGIANAGTGEPGLAAARATCEAV
                     AAELGLAADQVLPFSTGVILEPLPVERLVAGLPAARAALRADGWFDAAHAIMTTDTLA
                     KAVSRQVQVGGKTITLTGMSKGAGMIKPNMATMLGFLATDADVAQPVLDALLKEVTDL
                     SFNSITVDGDTSTNDSYILIATGQAGNGTIADTASADYAAFREAVLDVSIRLAQAIVR
                     DGEGATKFMTLQIGGGRDREECRKVGYAIGHSPLVKTAFFASDPNLGRILAAIGYAGI
                     ADLDVAALRVWLGSQGEEVLVAEQGGRAASYREEDGARIMQAAEITVRVDLARGDAAA
                     TLWTCDFSYDYVKINADYRS"
     gene            complement(38356..39786)
                     /locus_tag="PLH85_01715"
     CDS             complement(38356..39786)
                     /locus_tag="PLH85_01715"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB63302.1"
                     /translation="MTLPAHPLDLTGWIERIRGRDMPVFGRTVESLRTLLSDDRVSAS
                     TLAQVILKDAPMTAKVLRLANSAFFNTSRQTVSTVSRAIVVLGFNPVAQIALSVSLID
                     ALLSGGVRGRAEAEMARSFHAAVQARWAAAKRGEGHGEEVFIAALLTRVGEMAFWCFG
                     GDQARSLDGLLAQGRMREEDAQQVVLGFPLRHLSAGLVREWHLGALAQAVLEGQSRGR
                     NGEQAVLLGHRLARASEQGWDAPPARQAVQALADYLELPFAAVLDAARANAAEAARIA
                     SSFGAPAPPAGAAASAGSGPAQADPAAPPVGVADPHLQLHILHDVAAMTLAQAPLDDI
                     LQMVLEGLYRGVGCERVLVARLSADRSELQGRAAIGSGAEALAASFVFSMDGEPDDLL
                     DAAIDSAQGCWLTPSRAAQPGAERLLKVAGSRHAYLAPFGTGSRQVGVFYADGHGRAL
                     DEAGWHAFQHFVLQASLAVGLSLQGQ"
     gene            complement(39898..42624)
                     /gene="secA"
                     /locus_tag="PLH85_01720"
     CDS             complement(39898..42624)
                     /gene="secA"
                     /locus_tag="PLH85_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB63303.1"
                     /translation="MIAGLLKKIFGSRNDRLIRQYAQKVRVINALEPTVQALSDEALR
                     GKTAEFRQRLANGEALDALLPEAFAVVREAGKRVHGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLTATLPVYLNALAGKGVHVITVNDYLASRDSDWMGRIYRFLGMSVGCNLSQ
                     MPHELKKAAYEADITYGTNNEFGFDYLRDNMVYSLEERVQRGLHYAIVDEVDSILIDE
                     ARTPLIISGQAEDHTELYIRMNAVPPLLTRQEGEGEDITQPGDYTVDLKAHSVLLTEQ
                     GHEHAEEILGRLGLLPEGASLYDPAHILLIHHLYAALRAHALYHKDQHYVVQNGEVVI
                     VDEFTGRLMPGRRWSEGLHQAVEAKEGARIQAENQTLASITFQNYFRMYGKLSGMTGT
                     ADTEAYEFHQIYGLETVVVPTARPMVRKDQNDKVYRTMKEKHDAILADIQDCVQRGQP
                     VLVGTTSIENSEFLSDLLTRAKLPHNVLNAKQHAREAEIVAEAGRPGVITIATNMAGR
                     GTDIVLGGNVDKPIALLREDENIPDADKEAQISRLREEWKVRHEQVLQAGGLKIIGTE
                     RHESRRIDNQLRGRSGRQGDPGESRFYLSLEDPLMKIFAGERLNAIMVRLKMPEGEAI
                     EAGMVTRSLETAQRKVEQRNFDIRKQLLEYDDVANDQRKVIYQQRNELLESTDITETI
                     TAMREGVLHDLLRTYVPAESVEEQWDIPALELALQSENQLKLPVGDWLKAEPDLTDDD
                     LLQRILEAGNAAYADKISLVDAAAWHGFERNVMLQSLDTHWREHLSALDHLRQGIHLR
                     GYAQKNPKQEYKREAFELFENLLSVVRVEVTKLLMTVQIRSEAQLDEAEAPPELENVQ
                     YHHADYDEALATANPDAGVAQPAVNLTPKVGRNDPCPCGSGKKYKHCHGKLN"
     gene            42774..43202
                     /locus_tag="PLH85_01725"
     CDS             42774..43202
                     /locus_tag="PLH85_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNB63304.1"
                     /translation="MARALDAYLDRGDTLARLHAHAARLLRLQRTLSDVLPDYLAASC
                     QVANLKDEVLVIHTRGAAVAVKLRQAIPGLLADFARAGVALRDIKVKVAAPESPRPAA
                     APVHRSVSDGTRAGLDELAGRLPADSPLAQALRRFVRSAG"
     gene            complement(43206..43397)
                     /locus_tag="PLH85_01730"
     CDS             complement(43206..43397)
                     /locus_tag="PLH85_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63305.1"
                     /translation="MLLLRLIAVLVAIGIGASAFAYLFTRDRRYLAFALRLARYALIV
                     ALVFLALLALERVLAPAFG"
     gene            complement(43412..44329)
                     /gene="lpxC"
                     /locus_tag="PLH85_01735"
     CDS             complement(43412..44329)
                     /gene="lpxC"
                     /locus_tag="PLH85_01735"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNB63306.1"
                     /translation="MLKQRTLKSAISTTGVGLHSGNKVTLSLRPAPPDTGVVFHRVDL
                     DPPVDLPADPYAVCDTRMCSGLQKGTAKVNTVEHLMSALAGLGVDNVHIDVDAAEIPI
                     LDGSAGPFVFLIQSAGIEEQNTPKRFMRVIKPVEYREGDKWVRLEPYDGYRLSFSILF
                     NHPAIDRTGTSVTVDFAEHAYTREVARARTFGFMQDVEFMRANGLGLGGSLDNAIVMD
                     EYRVLNADGLRYADEFVKHKVLDAIGDLYLVGHPLLAAYSAYKSGHALNNQILRVLLE
                     DRSAWEIVTFEEDARTPPAVAHLFDLALA"
     gene            complement(44488..45627)
                     /gene="ftsZ"
                     /locus_tag="PLH85_01740"
     CDS             complement(44488..45627)
                     /gene="ftsZ"
                     /locus_tag="PLH85_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNB63307.1"
                     /translation="MFEIVEKASGGTSIKVIGIGGAGGNAVDHMIRQGVQGVDFICAN
                     TDAQALTRCLAPVKVQLGKTGLGAGSKPEAGRAAAQESREQIAAALEGAHMCFITAGM
                     GGGTGTGAAPVVAEVAKEMGVLTVAVVTKPFDFENRLRVAESGVEELSRQVDSLIVVL
                     NDKLLEVYGDDAGFEDCFRSADNVLTSAVGGIAEIINVPGLVNVDFQDVRTAMAEMGR
                     AMMGSAEAAGLDRARIAAEQAAVSPLLEGTELSGARCVLVNITASRSLKMSEVRDAVK
                     TVQAFAAPEAFVKYGTVFEENMEDRIRVTVVATGLGAVRAAVARKPEMQVLRTGTDNI
                     GVEVDYGNLELPAVIRRTSRTTVEAMSASGVSTLDIPAFLRKQAD"
     gene            complement(45768..46997)
                     /gene="ftsA"
                     /locus_tag="PLH85_01745"
     CDS             complement(45768..46997)
                     /gene="ftsA"
                     /locus_tag="PLH85_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNB63308.1"
                     /translation="MSKEYKELIVGLDIGTSKITCVVAELRPDGRLDVIGLGTQPSSG
                     LKKGVVVNIESTVDAISRVIQEVELMADCKIRDVYTGIAGSHIRSFNSNGMVAIKDKE
                     VSPMDVERVIETARAMPLPADQQILHILTQEFIIDGQDGVREPLGMSGVRLEVKVHIV
                     TGAVSAAQNIVKCVRRCGLEVLDLVLQPLASSYATLSEDEKELGVCLVDIGGGTTDLA
                     VFTQGAIRHTAVIPYAGDQITNDIAMALRTPTAEAEEIKIRHGVAMHTLTDPAEMIEV
                     PGVGDRPPRKLSRQALADVIEPRVSEIYELVQAELRRSGYEELLSSGIVLTGGSAVMQ
                     GMVELGEEVFHMPVRIGYPNYSGALAEVVCEPRYANAMGLVLEGAAQKRRGIQARQTR
                     NVGQVFSRMRSWFERNF"
     gene            complement(46997..47875)
                     /locus_tag="PLH85_01750"
     CDS             complement(46997..47875)
                     /locus_tag="PLH85_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNB63309.1"
                     /translation="MVDRRATSNVPPEAGAKPSSPRRPRPAARAQKRDGGGNGLWDRP
                     QLLNLIADLLLVAGAAGLGYAAVAGLSRLPAFPLKEVVVTTPLAQVTTEQLEYAARSS
                     LKGNFFTVNLEEVRNAFEKLPWVRRADVRRRWPGGVEVRLEEHVASAYWRVGDTGDMR
                     LVNRYGEIFSAASNAPMPIFSGPEGSSGVLLAKFDEFSHKLGPLGRGLVGVSLSAREA
                     WQLKMADGLTIELGHDQAKAPLDERIDRFVRYYPRAKAQLNMNVAVVDLRYPNGFALR
                     AVPVAAPAGKQDSKGK"
     gene            complement(47868..48782)
                     /locus_tag="PLH85_01755"
     CDS             complement(47868..48782)
                     /locus_tag="PLH85_01755"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNB63310.1"
                     /translation="MRSQFGKVAVLLGGSSAEREISLMSGKAVLAALQGAGVDAHAFD
                     PAETDLHILKEEGYERAFIALHGRGGEDGTVQGALELMGIPYTGSGVMASAIAMDKWR
                     TKLVWLASGLPTPRFAILTADSDWDAVVAQLGLPIFVKPVHEGSSMGATKVTEAAQLK
                     GAWELAAQYDSLVLAEEFVSGQELTAPFLGDRALPLVRIVAPDGNYDYQHKYFTDDTQ
                     YFCPSGLPEALEKDIQATIVRCAKVLGCRGWGRADLILQDDGRYTLLEINTAPGMTNH
                     SLVPMSARVSGLSFEALCLEILAGARLG"
     gene            complement(48794..50188)
                     /gene="murC"
                     /locus_tag="PLH85_01760"
     CDS             complement(48794..50188)
                     /gene="murC"
                     /locus_tag="PLH85_01760"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNB63311.1"
                     /translation="MKHKVKSIHFVGIGGAGMSGIAEVLVNLGYQVSGSDLGDNAATR
                     RLKELGARVMQGHAAENVQDADAVVTSTAVKGDNPEVIAARARQIPVVPRAQMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASILAEGGLDPTFVIGGKLNAAGANARLGKGDFLVAE
                     ADESDASFLLLTPVISVVTNIDADHMETYGHDFARLKQSFVDFLQHLPFYGVAVLCDD
                     DKHVRSIMPLVSKQIVRYGFSESANVRAENIHAEDGRMRFDVVRVNGTTSRISVVLNL
                     PGRHNVLNALAAIAVATEVGVSDEAIVKALADFKGVGRRFQRYGEVALPAGGAATVID
                     DYGHHPVEMAATLAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVLSTADALLLGE
                     VYAAGEAPIVAADGRSLARALRVAGRIEPVFVEHIEDMPQAILAAAQDGDVVITMGAG
                     SIGAVPGKLVNGEV"
     gene            complement(50199..51257)
                     /gene="murG"
                     /locus_tag="PLH85_01765"
     CDS             complement(50199..51257)
                     /gene="murG"
                     /locus_tag="PLH85_01765"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNB63312.1"
                     /translation="MPTLMVMAGGTGGHIYPGLAVADALRARGWKVVWMGNPDGMEAR
                     IVPASGYDMAWVRFTALRGKGLLRKLLLPFNLLRGFAQALAQLRRVRPDVVLGMGGYV
                     TFPGGMMAALIGRPVVVHEQNSVAGLANRVLAGVADKVMTGFPDVLRKGEWAGNPVRA
                     DIAAVAPPAQRFAGRSGPLRVLVVGGSLGAAALNDAVPKALAQIPPAERPQVTHQAGT
                     RQIDALRAAYRAAGVEGELLPFIDDMAERYAAADLVICRAGALTVAELAAAGVASVLV
                     PFPHAVDDHQTGNARFLADAGAAVLLPQTELDPARLAALLGSLNRERLLEMAEKARAR
                     ARPDATSRVADVCAGLAA"
     gene            complement(51257..52492)
                     /gene="ftsW"
                     /locus_tag="PLH85_01770"
     CDS             complement(51257..52492)
                     /gene="ftsW"
                     /locus_tag="PLH85_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNB63313.1"
                     /translation="MSLTASLSGFFSRHAGAASQSEASRAVRRLMRPLGQPRELDPLL
                     IWSAAALLLLGLIMVFSASIATAEGSRSFSYQPAYFLIRHTVFLAIGVIAGVVVFQVP
                     MRTWQEIAPWLFIAGVVLLLVVLIPHVGRDVNGARRWLPLGVVNLQPSELMKLFAALY
                     AADYTVRKLPYMGSFKKGFAPMAGVILFVGFLLLKEPDFGAFVVITAIAFGILFLGGI
                     NVRLFVLLAVVAAVGFVLLITFSEYRRQRILGFMDPWQDAYGKGYQLSHALIAFGRGE
                     WFGVGLGGSIEKLFYLPEAHTDFLLAVIAEELGFAGVFTVVVLFGILIHRAFAIGKQA
                     LVLERTYAALVAQGIGIWIGVQSFINMGVNMGLLPTKGLTLPLMSFGGSGILANCVAL
                     AILLRIDWENRQITRGGNA"
     gene            complement(52489..53877)
                     /gene="murD"
                     /locus_tag="PLH85_01775"
     CDS             complement(52489..53877)
                     /gene="murD"
                     /locus_tag="PLH85_01775"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNB63314.1"
                     /translation="MAADLTLILGLGESGLAMARWLARQGVALRVADSRERPPGVERL
                     RAEAPAAEVVAGPFSDALLEGVTRIAISPGLDPRQPLVATARARGLTVVGEMDLFVQA
                     LRDLGVRDATRLIAITGTNGKTTTTTLAGEMVRACGVDGVVAGNISPAALDVLMARLD
                     AGEALPAVWVLELSSFQLETHPALEAISATVLNVTDDHLDRYAGLDDYAATKAAVFQG
                     AGVQVLNREDARVAAMAVPGRRVYSFGVDAPAGPDDFGLRTDAGGEWLVRGDERLLAR
                     ADLPLPGNHNIANALAALALCEAAGLPRAPLLAALKAFRGLPHRVEKVARRADGVVYY
                     DDSKGTNVGATVAALEGLRARVVLIVGGDGKGQDFSPLKDAFARYARAVVLIGRDAPA
                     IEAAVAGCGVPLIPAADMDAAVLAADARAQAGDVVLLSPACASLDMFRNYAHRAQVFI
                     DAVRRLPGVRPA"
     gene            complement(53884..54972)
                     /gene="mraY"
                     /locus_tag="PLH85_01780"
     CDS             complement(53884..54972)
                     /gene="mraY"
                     /locus_tag="PLH85_01780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNB63315.1"
                     /translation="MLLELAQWLSKDARFFNVFGYITLRTVLAAGTALLLSLVFGPPV
                     IRWLAAKKIGQAVRDDGPKSHLTKAGTPTMGGALILISIAVTTLLWGDLRNKYVWVVL
                     LVTLGFGAVGWVDDWRKVVYRDPKGLASRWKYFWTSMVALGAATFLGLTATLPAQIEL
                     IVPFFKAVTYPLGVVGFIVLSYFVINGTSHSVNLTDGLDGLAIMPTVMVSAALAIFAY
                     VAGHMGFSKYLGMPYIPGAGELAVICGAIAGAGLGFLWFNAYPAEVFMGDVGALALGA
                     AMGTIAVIVRQEIVLFIMGGLFVAEALSVMVQVMYFKYTGGKRIFRMAPLHHHYELGG
                     WKETQVVVRFWIITIMLVLFGLSTLKLR"
     gene            complement(55033..56406)
                     /gene="murF"
                     /locus_tag="PLH85_01785"
     CDS             complement(55033..56406)
                     /gene="murF"
                     /locus_tag="PLH85_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNB63316.1"
                     /translation="MDLQGAAVLTRGTAVGGNVVFESVSTDSRALQSGQLFVALRGER
                     FDGHGFVAQAASQGAAAAMVDRAWAAANPVPLPLLVVDDTRIGLGQLAAGWRARFELP
                     LIGVTGSNGKTTVKEMCAAILRARARREGLDPDLAVLATRGNLNNDIGLPLMLLRLSG
                     NHRAAVIEMGMNHPGEIGYLTRLAAPTVALVNNAQRAHLEGMGSVNEVAREKGAIYEG
                     LSADGVAVVNADDAFAGYWKALNAGRRVLTFGLRPGADVTAQVTSHGFASELTLASPK
                     GAASFRLQVPGEHNVRNALAAAAACLAAGARPEDVAAGLGAFSGTPGRLHVRAGVGGA
                     TVIDDSYNANPDSMRAGIAVLAATPGRKILVVGDMGEIGDQSFQYHDEIGGYAKSEGV
                     DFLFALGEMSEVAARNFGDGGRHFQRVDELVAAVKAEMNSNTVVLVKGSRFMKMERVV
                     DAIAEKA"
     gene            complement(56406..57917)
                     /locus_tag="PLH85_01790"
     CDS             complement(56406..57917)
                     /locus_tag="PLH85_01790"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNB63317.1"
                     /translation="MSPEHQASDILARLAADGVEATRLCADSRQVLAGDIFVAYPGHR
                     ADGRRYIVDAVHAGASAVLWEREGYQWNEAVDVPNVPVFGLAHLSGYIADEFYGRPSQ
                     ALWTVGVTGTNGKTSVTQWLARAYAQLGRRCGLIGTLGIGFPGQLSATLNTTPDALTV
                     HRALADIRDQGGQAVAMEVSSIGLDQARLNGVRIDVAVFTNLTRDHLDYHGTMEAYGE
                     AKARLFAMPGLRSAVLNLDDRFGMEQARSLAGSGVEVIGYTVIPSNRDAAPAQRVLVA
                     ENLTITPVGLRFTARCGNVSADLFANVVGRFNASNLLAVIATLMASGIPFEDACKVAE
                     GLTPPQGRMQTLGGVGDPLVVVDYAHSPDALEQVLRAVRPTAEARGGSLTCVFGCGGD
                     RDPGKRPLMGEVARRLADRLILTSDNPRTEDPQRILQAIVAGVQGDVDIIPDRAEAIR
                     HAVMRAAADDVVVVAGKGHEPYQEINGVRLPFSDAEQTRAALEAWNDAQGGLS"
     gene            complement(57914..59668)
                     /locus_tag="PLH85_01795"
     CDS             complement(57914..59668)
                     /locus_tag="PLH85_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB63318.1"
                     /translation="MNKTQKGVTFNHNPLLRSELPAWRPRFVLLCLLGGSLALVGRAA
                     YLQGVNDRFLQAKGESRYARVLDVPATRGKITDRQGEPLAVSSPVKSIWAIPEDAKLT
                     PAQSRQLAGLLEMPVQDLNQKLGTDKDFVYLKRQVSPELADRIAALKLPGVHDQREYR
                     RFYPGGEVMAHVIGFTGVDDKGQEGIELAFEPQLAGKPGSRRVVKDRRGNVVEDVQSI
                     REPQDGREVALAVDNKIQYLAHAALKQAMTDHKAKAGGIIVIDSRSGEILALANAPTY
                     NPNNRTKLTGAQLRNRALTDTFEPGSTMKPFSAAMSLEAGKVTANTIINCAPGRMTIG
                     TATISDAHPHGALTVAQVIQKSSNIGAAKLALAFPPETMWKMFDDLGFGAPLHLGFPG
                     EVGGRLRPAKSWRPVEQATMSYGHGISVSLMQIANAYQVFARDGDMVPLSLARVDAPP
                     VHGKQVFSPHTAREVRQMLELVVQQGGTAPQAAIPGYRAGGKTGTAMKLEGGHYTTKY
                     VASFVGIAPISDPRLIVAVMIDEPTNGKHYGGDVAAPVFAQVMGGALRTLGVAPDAPL
                     KPLQVARQSVVPAVKESM"
     gene            complement(59665..59931)
                     /gene="ftsL"
                     /locus_tag="PLH85_01800"
     CDS             complement(59665..59931)
                     /gene="ftsL"
                     /locus_tag="PLH85_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNB63319.1"
                     /translation="MARLTGFLIMLIVASSLGVVASQHRARKLYAAVEREQQRTRQLE
                     VEWDQLQLEQSTWAAHARVEKLAREKLSMRAPQPGQIVAIEERP"
     gene            complement(59933..60865)
                     /gene="rsmH"
                     /locus_tag="PLH85_01805"
     CDS             complement(59933..60865)
                     /gene="rsmH"
                     /locus_tag="PLH85_01805"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNB63320.1"
                     /translation="MTTSAPHVTVLLEEAVEALAVKPDGLYVDGTFGRGGHSRAVLAK
                     LGHQGRLIAFDRDPVAIAAGRGLDDPRFELVHAPFSDFAEALDARRVAQVDGVLLDLG
                     VSSPQLDEAERGMSFRFDAPLDMRMDTSRGETAAEWLAQAPVAEITRVLREYGEERFA
                     YEIAKALAAARAGGHVATTGQLAAIVEKAVRTREPGQHPATRTFQALRICVNRELEEL
                     SLILPQAVTRLVPGGRLVVISFHSLEDRIVKRFMRDESRPPQLPARLPLRAAELPRPR
                     LTLVGRQVRPSDAEVAANPRARSAVMRVAARTEA"
     gene            complement(60862..61308)
                     /gene="mraZ"
                     /locus_tag="PLH85_01810"
     CDS             complement(60862..61308)
                     /gene="mraZ"
                     /locus_tag="PLH85_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNB63321.1"
                     /translation="MFQGAAALSLDAKGRLAIPARHRDALAAQGDGQLVLTAHPHRCL
                     LIYPAPAWEPIRDKVLAGSSLEQHSALLKRLLVGFARDEELDSAGRVLIAPELRAFAQ
                     LDKQVHLVGQGNHFELWSDAGWQKQQEQIFALGDQLMPANWDALPL"
     gene            complement(61690..62077)
                     /gene="rnpB"
                     /locus_tag="PLH85_01815"
     ncRNA           complement(61690..62077)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLH85_01815"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(62124..62540)
                     /locus_tag="PLH85_01820"
     CDS             complement(62124..62540)
                     /locus_tag="PLH85_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63322.1"
                     /translation="MDVRRLVMGLGVLIVVGVGAVAVLGPELSGARAANGGGALAQAV
                     ESEYGDALRNAGVQSVGVDPRTQDVVVVFADVRPGGRPVGRLAGLAGPEGCGETDLGI
                     EVAGQITAWGQAGVRRERMRCVLDAVVTRMAVLRGS"
     gene            62765..63235
                     /locus_tag="PLH85_01825"
     CDS             62765..63235
                     /locus_tag="PLH85_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63323.1"
                     /translation="MNPRTIRRALLAAAAGIALMATGAAFADRGHGGGWHGAPGGWAA
                     RPAPGGYWHYGEHRGAWGWWWVLPGAWWLYDRPYYPYPAAPPVVVQPVPQEPVIVQPA
                     PQPAAQTWYFCESARAYYPYVQTCAEGWRPVPAAPPPASGAQQSAPAAGSGGVQ"
     gene            63232..63771
                     /locus_tag="PLH85_01830"
     CDS             63232..63771
                     /locus_tag="PLH85_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63324.1"
                     /translation="MNVRLAVLPLLLLAGCATLPDGPRVAVMPAPGKPFDLFVQEERA
                     CRAFASQSLGTTPNDLAARNVAGTAIVGTAVGAAAGALAGGHNGAGAGAAVGMVAGTA
                     AGASDARYAGADAQRRYDIAYQQCMYAKGNQLPGAYGYGSYRPASPYGTPPPAYPSGQ
                     PGYSIPPPNYPPPPPLRRE"
     gene            63818..64981
                     /locus_tag="PLH85_01835"
     CDS             63818..64981
                     /locus_tag="PLH85_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HNB63325.1"
                     /translation="MKFDVLVVGAGLAGAALAAALRGCSLKVGVVEARAPSRPTGWDP
                     RIYAISPANVAFLTSIGIWGHLDAARMAPIHDMEIHGDAGGRLDFSAYDCGLGELAWI
                     VESSLMQLELWETIKRQHNVTLLCPAAPAALALDEDAACLTLADGRRIEARLIVAADG
                     RDSWVRQAAGIDVRNTPYEEKGVVANFRCEKPHRNTAFQWFRKDGVLAYLPLPAQQIS
                     IVWSAPDDLADQLVALSPEALAVRVAEATDARLGALETLTPAAAFPLRLMRVERVVQP
                     RLALIGDAAHAIHPLSGHGINLGYQDARVLAEQLAALPAWRDPGELAVLRPYARARAE
                     EAALLQYTTHGLNRLFKPANPLLSGLRNLGLNLTNRLPVLRNALVRYAIAGRF"
     gene            65014..65739
                     /locus_tag="PLH85_01840"
     CDS             65014..65739
                     /locus_tag="PLH85_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNB63326.1"
                     /translation="MKSASFRPLALALLLVTGSALANDEAAVRKNLDTFFGGQAVDTV
                     KKTPYGGLYEVVLKSGELVYTDAAVSFLLDGNVIDTKTKQNVTQARMAQLMKIDFATL
                     PLDQAIKQVRGTGKRVIATFEDPNCGYCKRLAKDMVNLKDATIYTFLYPILGPDSLEK
                     SKGIWCAKDRAAAWTDWMTAGKAPAAGSCDTAVLDKNVALGQKMRVSGTPTIFTVDGN
                     RLPGAVPLAQLDKAMDQAATGKR"
     gene            complement(65776..66615)
                     /locus_tag="PLH85_01845"
     CDS             complement(65776..66615)
                     /locus_tag="PLH85_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB63327.1"
                     /translation="MPLTELVRHLNHRHALRGGEGRLDLVEGRAVLRVGDWLLGSEFQ
                     PVVDARSGHAIGHEARLAVQAGPAGLPSQALFLALEHVEAVIQVDRLCRHLHALNFLV
                     EPARRGFLSLNVHPCTAAHWGVHPVTVLDPVLMRCGLLADQLVLEFPDDGFGSALPLA
                     AAIDACRREGHRVALDNFGRHSADLRRVEALMPDIVKLDSSIIGHSGHLSLARRVLMG
                     LAAEFRRMGVVAVCQWIENPLQLQVARDVGVDWLQGRLLGRPTGRCVPVMPEIPLREP
                     AAA"
     gene            complement(66714..67958)
                     /locus_tag="PLH85_01850"
     CDS             complement(66714..67958)
                     /locus_tag="PLH85_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HNB63328.1"
                     /translation="MDRASLSRLLAPASAVLLLAACASAPKSVEAPRPAPPSVPAAPA
                     VPAPPGAAAAPTTPLARPLLAADWRDLPGWGSDAHDEAWPAFLQSCRSLGRQATWQGV
                     CDAAKGLGEKPSASAARSFFESRLTPWRVTNPDGTKEGLITGYYEPLIQGCRTRTAVC
                     AWPIHATPDDLITVDLADAYPDLKPYRLRGRLVGRKVVPYYSRAELDRQPEKLAGKVL
                     LYAADPVDLFFMQVQGSGRVQLPDGGLVRLAYADTNGHPYQSIGRWLADKGELRLDQA
                     SMDGIKGWARANPGRLKEMLGANPSYIFFREVPNTPGGPAGALGLPLTDGRSIAIDPR
                     SVPLGAPVFLATTHPRSAQPLQRLVLAQDTGTAIKGGVRADFFWGFGAEAGALAGRMK
                     QKGEMWVLWPRDQVPRAANGSL"
     gene            68132..68995
                     /locus_tag="PLH85_01855"
     CDS             68132..68995
                     /locus_tag="PLH85_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB63329.1"
                     /translation="MTDLNDLIARAGQLLDRLEGLLPAAPTAPDWHAAVAFRWRRSRS
                     AATLQPVLRPHAIRLDDLQDIDDKKARIVANTRQFLAGKRANNVLLTGARGTGKSSLV
                     KAVLNEFADAGLRLIEVDKDDLIDLPDIVELVQGRPERFILFTDDLSFEDGEPAYKAL
                     KSVLDGSVAAVPDNVLIYATSNRRHLMPEYFAENAATKHKDGEVHPAEAVEEKISLSE
                     RFGLWISFYPFSQDEYLAVVNHWLREFGVPAEAVADARGEALQWALLRGSRSGRVAWQ
                     FARDYAGRFDA"
     gene            68992..69945
                     /locus_tag="PLH85_01860"
     CDS             68992..69945
                     /locus_tag="PLH85_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNB63330.1"
                     /translation="MSASPPRKIVHVAAAVITRPDGSYLLGQRAPGTFYPGYWEFPGG
                     KVEAGETPRDALVRELAEELGITVETAWPWIVREHVYEHAHVSLHFFEVPAWHGELTD
                     KVHSALAWQHPDAVDVTPVLPANGPILKGLRLPRRMGITQAGAVGVERQLKRVDAALA
                     AGLRLVQIREPELDAEMLACFAREVVARCHAVGALAVLNGDAELAHTLGADGVHLPAR
                     CLMSLAARPDLDWVGASCHTRAELERAAALGLDYAVLGAVQATATHPERKPLGWSGFT
                     TLAAGLPLPVFALGGVQRDDLETARAAGAHGIAAIRAAWDT"
     gene            complement(69985..70185)
                     /locus_tag="PLH85_01865"
     CDS             complement(69985..70185)
                     /locus_tag="PLH85_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNB63331.1"
                     /translation="MSTPVRSREVKCPTCGKAVLWVPENAFRPFCSERCRKIDLGAWA
                     SDTYRVAGETPLDDGADPLTRT"
     gene            complement(70182..70937)
                     /gene="zapD"
                     /locus_tag="PLH85_01870"
     CDS             complement(70182..70937)
                     /gene="zapD"
                     /locus_tag="PLH85_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNB63332.1"
                     /translation="MISYEYPLNERIRTLLRLEDLFDKVMHFVRADGSLEHHVALVTL
                     FEILEVASRADLKVDLVQELERQRQILLSFRNNPQISEEALSGALYEIEQASASLLAM
                     AGKTGQYLRENEWLMNIRSRAAIPGGACEFDLPSYHHWLNRDVEFRRRDLVAWVQPLL
                     PIRDGLAIVLRLLRASGRPEELVAARGAYQQTMGGRTVQMIRIRLSSGDPYVPETSAN
                     KYALNIRFMHAETVVRPRQSEADVRFELTFCNL"
     gene            complement(70991..71614)
                     /gene="coaE"
                     /locus_tag="PLH85_01875"
     CDS             complement(70991..71614)
                     /gene="coaE"
                     /locus_tag="PLH85_01875"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938351.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNB63333.1"
                     /translation="MSSGPFVVGLTGGIGSGKSAVADRFATLGAAVVDTDRIAHALTA
                     PEGAAMPALRAAFGEGIVAADGALNRAAMRALAFEDASVRKRLEAILHPLIRDESARQ
                     CAVAVAPYVVLVVPLLIESGTYRERVQRLCVVDCPEALQVERVMCRSGLDERQVRAIM
                     AAQATRAARLAAADDVVDNAGSLEALQARVAALHGEYVQCAARGRVL"
     gene            complement(71611..72351)
                     /locus_tag="PLH85_01880"
     CDS             complement(71611..72351)
                     /locus_tag="PLH85_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB63334.1"
                     /translation="MVPLRVAVIVLIFANLLALALWKGWLGGGDSDGQPERLSNQLNA
                     ERLKLAVDARPPAPRPREAIAEAPRTSPEPPVPTPAAPAQRESAPATDTSAPPACVAF
                     AGLGVDQAQELAARIGKVGGGLKLNESRSEQPSSWWVYIPSPGSKDGADRKAAEVRKL
                     GVDDLYIVPDAGPNQFAISLGLFKSEVAANRQLEVLRAKGVRSAQIATRGSALVRLEV
                     RGPSDSLATLVSDLSDHVKGVSRQGCKP"
     gene            complement(72407..73126)
                     /locus_tag="PLH85_01885"
     CDS             complement(72407..73126)
                     /locus_tag="PLH85_01885"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNB63335.1"
                     /translation="MNLLIDAGNTRVKWGLHDGAVWHARGAVPTATPEALEAALAVQP
                     VPARVMGANVAGASVALAVGRLCAAWKRTPGWLVPGIEAGGVRNAYLDPTQLGPDRWA
                     ALIGARTLHAGAALVVTAGTATTVDVLTAEGVFRGGIILPGLELMRRALARDTARLPF
                     ADGRFDATPRRTADAIVSGCLHAQAGAIERMFRLVADAPDAVCLLNGGAAADLGPQLG
                     CPVRQVDNLVLEGLARLTSGK"
     gene            complement(73123..73920)
                     /locus_tag="PLH85_01890"
     CDS             complement(73123..73920)
                     /locus_tag="PLH85_01890"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNB63336.1"
                     /translation="MSCEPLDRDAVLAVLGDHAAAFSVVVVPECASTNSVLLDDPPAD
                     DGRIHVLVTDRQTAGRGRRGRNWLSWPGAGLTFSVLWRFEPGAPVPAGLSLVAGLAVA
                     RALEELGVEGVQLKWPNDVLIHGRKLAGILVELIPGRGRTPAAVIGVGLNLRLPPEAD
                     IPHAGGVTDLAACLTAPPTRARLLGCLLDQFRGLLELYAAAGFPALRGAWQQRNAHAG
                     LPVRILGETVSQEGTCVGVDEDGALLLNTGSGLQRVLSGEVSLRSVA"
     gene            complement(74154..>75163)
                     /locus_tag="PLH85_01895"
     CDS             complement(74154..>75163)
                     /locus_tag="PLH85_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB63337.1"
                     /translation="SAVPEILGQAPSMQEVFRAIGRLSQSHATVLINGESGTGKELVA
                     RALHRHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTL
                     FLDEIGDMPAELQTRLLRVLSDGNFYRVGGHQPIKANVRVIAATHQNMEERVRQGLFR
                     EDLFHRLNVIRLRLPPIRERREDIPMLAKHFIALSAQELGVEPKRLSDATLKYLQSLD
                     FPGNVRQLENLCHWLTVMAPGQTVEVADLPPELKEQPTREQPANWVDALTAEADRALV
                     QNPGEVFDRLSRDFERILIRRALNATGGRRIEAAQLLGIGRNTITRKIQELGLEEGEH
                     A"
BASE COUNT        12146 a        26006 c        25340 g        11471 t
ORIGIN      
        1 taacgtgcta ggtcaggggc gcgaagccgg cttgccggcg gagcgtccct ctggagcggt
       61 gggttgggca tcatagccag aggaggctag gtcatttttc attatttttg gcagcagcta
      121 cagcaactgc ggttgctaca gccacaccaa cttttaatgc agcttttcca aactcggaag
      181 cgagattaga tactgtttct gtgcacgaag ggcaaagctc atggtcataa acagaggtcc
      241 ctttggcaaa gtcgtggcag ccaggtgagc ggcaaagata gatttcttct ccgcactctg
      301 ggcatttgca acgatctcgc cgaattgtgt tttcctcgac aatgaccggc gctactacct
      361 tttcacagaa gggacacttt cctttttgct ttgtagtcat ttgactatct cctgatgatc
      421 tcagcgtggt tcttttgtga tgcccaacgt gaggtagacg acatctgacg ctcttggcgt
      481 cttagacgac tgacgcctaa accgccagga tgcgccaagt cactgtattg atacatatct
      541 tatccgaagt tttcgatcat gatgtcgtct acccttccag aagagcctcc tgcaatccct
      601 tctgttgaat cagcttgtag cgcggatcag cgatccgtct gaagacggat tcagtctaca
      661 ccgtatggat taaacgccat gcccgctttc atgacaagcg gtacgccctg gacaccgatt
      721 cacaaaggag ccccccagcc ctgcggaaac gattcacccg ccgtaggaag gattaacccc
      781 cgttcgcaga atgcaacccc atacgatcta aggacgcgaa gcaggcggca atccactaca
      841 ttcacgccgt tgcccgcttc aacaaagtcc gacgcccagc cgccacgcgc agcggcaagg
      901 cgtctccgtt catgtcagcc caaacccctc ctcactccgt cggtttcggc ttcggccctc
      961 ggctcggtga cttcttgtcc gatttcttct tcaagcccag cgcggccacg tgatcggaat
     1021 ccggtccgaa cattgcgcgg gcctggtctt tggcgccgag gatgatgctg tggaagtccc
     1081 attgctggct gaccagggcg tcgcgggcgg cgtcgagggt gtgctgggcg tggagttcgg
     1141 cttcctgggc ggcgcgcagg gcgtcgtggg cgcgggtgat ggcgtcgacg gcgtattcgg
     1201 gtttgatggg gttccagtcg gggatgacct tgatggcgag gtaggcgtcg atgtcgtcct
     1261 ggacccgttg cgggggcagg cggtttttgc cggcagcagt catggatgtc tccttcggtg
     1321 gggtgaaagt cttctctgtc ataaatgaca gcgcaatcat atccaatacc gcgaagatga
     1381 caatcgggca agccatgcca tgaggcaccg ccgatctcat ggtttttaat ggcgcccatc
     1441 acgcagcaca atactgtctg gccgtccgga caggcctgtg ccgatgtgcg aagacgctgc
     1501 gtgcctgcgc acgctgtctg ttgagggcgg cgatgggtga aggatgggcg tccaacacac
     1561 tgtgcacagc ccgacagtgt ctgcgcaggc gtcgacagac gatgtggacg tggacacaca
     1621 cactgttgat agccctttac ctttgtttga ggcctcagaa cactggaaag aactatcgat
     1681 ccttgatgtg gacggcgctt acaagactgt cgaccggtcg acagaggctg tgcacgggcc
     1741 aacagacagt gtgcacccgt gcacactgtc cgagcacgcg tcatcatgat cttcgctgcc
     1801 cgcgacagcg tctgagcgca ggtccacatg atctgaggcg ccgtgcgcag acgctgcggt
     1861 cggcgccacg gcaagggtgg aggtgtccgc ctgcactgcg ggggccgacg cggcctggtg
     1921 gcttccgtcg gcccctgctc cggccccagc gcccgggtgg cgccggcccg accccgcccg
     1981 gtaccggccg gcgcagacga catccccacc ccagcgctgc ggtcggccgc gtcatcactg
     2041 cgtcggcggc cttcaggcac aataaggccc tctgccgtct atcgccgtca tgcctcccct
     2101 gccccgcagc atcgcccacc tggacatgga cgccttttat gcgtccgtgg aattgttgcg
     2161 cgcgccggaa ttgcggggcc tgccggtggt ggtgggcggt cggcgcgcgc tgacggccgc
     2221 cgcgggggag acgcacccac gcctgcggga ttacgtgggg cgcggcgtga tcaccaccgc
     2281 cacctacgag gcacgggcgc tgggcgtgca ttccggaatg gggctgatga aggcggcgca
     2341 actggcgccg gacgcagtgc tgctgcccgc ggatttcgag gcctaccggc attattcgca
     2401 acgcttcaag caggcggtgc gggcgattgc gccggaggtg gaagaccggg gcatcgacga
     2461 gatctacctg gacctctctg ccctcaaccc ggacggcagc gtggcaggcg cccgcgccct
     2521 ggccctgcgc ctgcagcagg cggtgcgggc agccaccggc ctgtcctgtt ccctgggcgt
     2581 ggcgcccaac aagctgctgg ccaagctgtg ttccgacctg gagaagcccg ccggcatcac
     2641 ggtggtgacc ctggacgacc tgcccacccg catcggcccc ctgccggcgc gacgtatcaa
     2701 tggcatcgga cccaaggcgg cggcccgcct ggagcagctg ggcatcgaca ccatcgccca
     2761 gcttgccgcc gccgatctgg acctgctcca gcatcacttc ggcgagcgct acggcctgtg
     2821 gttgcagcgg gcagcgcggg gtgaggacga gcgtccggtg gtcacccgca gcgaacccaa
     2881 gagtctgtcc cgggagacca ccttcgagcg ggatctgcat ccccgcagcg accggcagga
     2941 actctcggcc cgcttcaccc gtctgtgcga acagctggcg gcagacctgg cgcgcaaagg
     3001 ctacgtgggc aagaccatcg gcctcaagct gcgcttcgac aacttccaca ccgtcacccg
     3061 cgaccacacc ctggcggagc ccgtcggcga cgcgccgacc ctgcgccggg tggccggcga
     3121 atgcgccagg cgcgtgcccc tggagcgacg catccgtctg ctgggcgtgc gcgtctccgg
     3181 cctggtgcgc cgcgatgcgc tgccgcccgc cgaatcccga ccggcccagg ccgagctgtt
     3241 tgcctgagcc catcaccccg acccgagccc ttctccggag aagtccatgc cccgccccct
     3301 ccccgacgct gccgacgaac cctggcgcca gaacctgcgc ggcgaacacg acgccctgct
     3361 gcagggcgcc cggcccgact ggtggtggac gggcctgccg cccaccgatt gccccggccg
     3421 ccagcccgac ggccgcctca ccgcgctgcc ggcgcccaac ctggccacct gtacgcggga
     3481 cgaggtgctg gcggtcttcg acaacggctg gacgctcacc gaagtactgt ttgcgggcct
     3541 gcagggcgaa gaggcgttct accgcccgcc ttatcatcac ctgcgccacc cgatgatctt
     3601 ctactactgc cacccggcgg cgctgtacat caacaagctg cgggtggcgg gcctcatcga
     3661 cgcgcccctc aacccctact tcgagcgcgt cttcgagacc ggtgtggacg agatgcgctg
     3721 ggacgacatg tccaagaacg ccatgctgtg gccgtccttt gccgaagccc acagctaccg
     3781 ggcggaggtg tacgccaccg tgcgccgcgt catcgagacc cacccggatc tggccaccgg
     3841 ccacgcgccg ctgggcatgg atcatcccct gtgggcgctg ttcatgggct tcgaccacga
     3901 gcgcatccac ctggagacct cgtcggtgct gatccgcgag ctgcccatcg ccctggtgca
     3961 gccgccggac gtgtggcccg ccgtgcatcc cagcgccgcc gcggcggcca cggccgagtt
     4021 tccgccccgc gcgggcgcgg actatcccgt cgcccgctgg gtggacgtcc ccgccgccag
     4081 cgtgcgcctg ggcaagcccc gcgactggcc cagcttcggc tgggacaacg aatacggcga
     4141 gcgccgcgtc gcggtgcagg gctttcgcgt ggaggaccag ctcgtcagca acggcgcctt
     4201 cctggaattc atccaggccg ggggctatct ggagccgcgc tggtggagcg aggccggctg
     4261 ggcctggcgc agcttccgca acagcaagtg gccgtgcttc tgggtgccgg acggccccgc
     4321 cggcctgcat cgctaccgcc tgcgcacggt cttcacgatc gtgcccatgc cctgggcgtg
     4381 gccggccgag gtgaatgccc acgaggccgc cgcctattgc gcctggcgca gcgagcgcga
     4441 cggccacccc tgtcgcctgc ccaccgaggc ggagcacaac gccctgcgcg cgccggcctg
     4501 ggcggccgag gacccggcct tccgccagga cggcgccgcc ctcggccgcg acaccggctg
     4561 gaacctgcag ctcgcctggg gctcgccgtg gccggtggat gcgggcacgc ccaccgccgc
     4621 tggcgtgcgc gacgtgttcg gcaacgtgtg gcagtggcac ggagacgatt tcaaccccct
     4681 ccccggcgcc cgcgtccatc cctattacga cgacttctcc acgccctgct acgacggcca
     4741 gcaccagatg ttgctgggcg gctcgtgggt ggcctgcggc gacgaggcca gcgtgtgggc
     4801 gcgcttccac ttccgtcccc acttcttcca gcacgccggc ttccgcgtgg tgcaggcgga
     4861 gcacgacggc ggcgtggtgc gcctgggggc ggcggacagc ggcgcccagg tatacgagga
     4921 cgcgcgcatg gtggacgact acatggtcct ccaccacgcc cgcccggagg cccagatgcc
     4981 ctggccggcc ggcccgcggg gggccacggg ctttccgcaa cgctgtgcgg actggctgct
     5041 gaagggcgcc gcccagcacg ccagcggcac cgcccgcgcc ctggacatcg gctgcgcggt
     5101 gggcggggcg agcttcgcgc tggcgcgggg ttttgacgcg gtgctggggg tggatctgtc
     5161 gcgcgccttc atcgacgcgg ccagcaccct gcagcgggac ggccggcgtg actatgtcct
     5221 gcgcgacgaa ggggaactgg gcgacaccct gagcgcggtc atcgacccga ccctcgagcg
     5281 cagccgggtg agcttccgtc aggccgatgc gtgttccctg cccgccgagc tggcggactt
     5341 cgacgcggtg ctgctggcca atctgctgtg tcgcctgccc agtcccaagt cgctgctcgg
     5401 tcgcctgggc gggccgcggg gcctggtcaa ggtgggcggg ctggtggcgc tgttctcgcc
     5461 ctattcgtgg ctggagcaat tcacgccgca ggaggcgtgg ctcgggggtt tcacggccga
     5521 cggccgcgcg gtgcgcagcg gcgacgcgct gaaggccttc ctcaccgaag aaggctttgt
     5581 gctgcgccgc gaggaggacg tgcccctggt gatccgcgag cacgcccgca agtatcagta
     5641 catcgtgacc cacgggatgc tctggcagcg ggtgcgctga ggggcgctgc atccggatgc
     5701 cggccggctg cgtagaaggg ggtggaagac acccccccgc aaccttcagg agatccgatc
     5761 atgagcaccc tcatccagcc cgcccgccgc ggctttctgt ccacctccgg caagaccgcc
     5821 ctgtccgtgg ccgccctcgg cctgctggcc ggccgcagcg atctggcgtc ggccgccagc
     5881 ggcaatgccg ccggcgacgt ggccatcctc aacgtggccc tcggcctgga acacgaagcc
     5941 atcaacgcct accagctcgg cgcgggcagc ggcctgctgc agaagccggt gctggacgtg
     6001 gcggtgctgt tccagtccca ccacaagggc caccgggatg cgcttatcgc caccatcgag
     6061 aagctcggcg gcacgccggt caaggagaag tccctggacg agtacgcccg cgccctcaag
     6121 gcggacacgc tcaagaacca ggccgatgtg ctgtccctcg ccgcgcgcct ggaactgggc
     6181 gccaccaacg cctatctggg cgtgatcccg tccttcaagg atgcccagct cgcccaggtg
     6241 gccggccgcc tcgccgccga cgagaccatg cactggaccg ccctgagcag cgccctcggc
     6301 cgcagtctgc cgggcggcgc cctcagcttc ggcgcctgag caggtcgtgg gcgcccggca
     6361 cttcgccctg gtcgccgggc tcgccctgat cggcccggcc accgcccagg atgccgcccg
     6421 cggcgcgcag ctctacgagg cgcgctgcgg cggctgccac tcgccggagg cgaaccgcat
     6481 cgggccgcgc cacgccggtg tcctcggacg ccgtgccggc agcctgccgg acttcgccta
     6541 ctcgccggcc ctggcccggt ccaccgtgat ctggcgcaac gacaccctgg aacgctggct
     6601 cgccgacccg gaagccctga ttccgggcca gcgcatgaac taccgggtcg ccgacgccca
     6661 ggaccggcgc gatctggtgg cctatctggc gagcctgcaa gtggttgcgc cggacggcgg
     6721 acgccgctga cgccggcaat cccgcggcgg agtcggccgt gacatgcgcg ggcatcgcgc
     6781 cgggccgtgt cgatggcgcg gacacggcgc gggcgcggtg ccgagcgtcc gacgaagggg
     6841 acgaagactc tgccggcgcc gccgcagacc gggtagaccc gcgcgcaggg atttggcaga
     6901 tcggctacag gtcatcgccg tcgctcacca tggccgggcc ctgcccgcgc gacggacgcg
     6961 cggggctcag gtgcgcagga gctgggccag accggcctcg cccacgggcg cctccgccag
     7021 ccaggggatc agcgcacagc gcggcgcgag ttcgtccgcc acgcggcgga tgaaggggat
     7081 ctcccaggcg ccgcgctggg acagcagcgg gtcgcgcgtg ccgctcgcca gcagggactg
     7141 gttgatgacc cacgcgaacg gggcgatgcc ggcccgcccc aggtcgtgct ggaggcgctc
     7201 cgcctcgtgc accggggtgg cttcggcgag ggtcacgatg agcacgtggg tgtaatccgg
     7261 atcgcgcagc cgcggcagca actcgcgcac cgactcgggc atgtcgccct gggtgcgcat
     7321 cacttcccga tggtaggcct cggccgcatc cagcaacagc agggtgtggc cggtgggggc
     7381 ggtgtccagc accacgaagc cctcgcggcc cgcctccacc gtgcgcgcga aggcgcggaa
     7441 cacggcgatc tcctcggtgc agggcgaacg cagatcttct tccagcaggg cccgtccgcc
     7501 ggcatccagc tgggcgccgg ccttgtccag cacttcacgg gtgtagcggg ccacttcctc
     7561 ggcggggtcg atgcggctga cgctcaggcc gggcacctgg ccgtcgaggg tgctggccac
     7621 gtgggcggcg ggatcggtgg tggacaggat caccggatgg ccgcgacggg ccagggccac
     7681 cgccacggcc gccgccaccg tggtcttgcc gacgccgccc ttgcccatgg tcatgatgac
     7741 gccgtgaccg gcggcggcga gggtctccac caggccggcg aggccgggcg gcagcgccgt
     7801 ctgcgccgcg tcgggcacgg tgggggcggc gacggactcg ggatgggcca tctgccgcag
     7861 ggccgccaag cccaccgtgc cgcggggcag gaaggggatc gtggtggcgg gcagcgcggc
     7921 cagaccggcg ggcatgtcgg ccagggcgat ggcggcacgg cgggacaggg ccgtggcgac
     7981 ggcgtcgtcc tggcgttcgg tgcggaacac gccgttgagg gccagcagca gattcgagac
     8041 gccgagcccc gccagctcgt ggcgcgtgcg ctcggcttcg cgcagggagg cggcctcggc
     8101 gcggctgacc aggacgacgg tggtgtcggc cgcgctggag aggcgcgcca cggtggccgc
     8161 gtagagggcc ttctgctgtt caaggccggc cagcggcccc aggcaggagg cgccgcccgt
     8221 ggacgacgcg atgaacccgc tccacgccga cggcagggtg agcagacgca gggtgtggcc
     8281 ggtcggggcg gtgtcgaaga tgacgtggtc gaaatccgtg gtggcggccg cgtcgcccag
     8341 cagcttggag aattcgtcga aggcggcgat ctccacggtg caggcgccgg agaactgctc
     8401 ctccatgctg cggatggccg ccgccggcag gatgccgcgg tagggcgcga ccatgcgctc
     8461 ccggtaggcg cgggcggcgg cctccgggtc gatgttgagg gcgaacagcc ccggcgcgcc
     8521 gggaatcgcc gtgggcagcg ggcccagggc cgcgcccagc acctcgtcca ggttggacgc
     8581 cgggtcggtg ctgacgatca gcaccttctt gccggcgtcc gccagcgcca ggccggtggc
     8641 gcaggacagg gacgtcttgc caacgccgcc cttgccggtg aagaacaggt agcgggtgcg
     8701 cgaatcgggg agggccatgg cggcgccctc agcaggagcc cggcttgcag ccgcagccgc
     8761 cgggcttgat gtggggcttg tcggcggtcg tcagggcgat gcccagcttc tgggccagtt
     8821 gcacccggga cgggtacatg ccggtggcga cgatgtggcc atccacgacg aggatgggca
     8881 ggcggtccat gccggcttcc atctccttga gcaccgcggg attggccgcg aaggcctggg
     8941 gcgcctgacc caggttgtga cgctcgacgg cgatgccgtg ggcttcgacc cactgcaggt
     9001 cggcagcaaa ctgggccagc accgggtcca cgtccacacc gcagacgccc gtggaacagc
     9061 acatggcggg atcgaagact tcgagtttct tcatgatgcg ctccttacgt gtgatgggct
     9121 tcgtaccagc ccttggaagc attgaccacc ttgaccacca gcagcatgac cggcacttcg
     9181 atgagcacgc ccaccaccgt cgcaagcgcc gcaccggatt cgaagccgaa caggctgatg
     9241 gcggcggcca cggccagttc gaagaagttc gacgcgccga tgagggccga cgggcaggcg
     9301 acgctgtgcc gctcgcccac cagccggttg agcccgtagg ccagcgccga gttgaagaac
     9361 acctggatca ggatgggcac cgccagcagg gcgatgacca gcggctgacg caggatggcc
     9421 tcgccctgga aggcgaagag cagcaccagg gtggccagca gcgcggcgat ggaccagggg
     9481 ccgatcttcg ccatcgccgc gtcgaaggcc gccggcccgc gggccagcag ggtccgtcgc
     9541 cacagctggg cgaggatgac ggggatcacg atgtagagga ccaccgaggt gagcagcgtg
     9601 tcccagggca ccgtgatgga cgagaggccc agcaggaagg ccaccagggg cgcgaaggcg
     9661 aacaccatga tggtgtcgtt gagggccacc tgggacaggg tgaacagggg gtcgccgtcg
     9721 gacagccggc tccacacgaa gaccatggcc gtgcacggcg ccgccgccag caggatgagg
     9781 ccggcgatat agctgtccaa ctgctctgcc ggcagcagcg gcgcaaacag ctgacgcacg
     9841 aagagccagc ccaggaaggc catggagaag ggcttgacga accagttgac gaacagcgtc
     9901 acgccgatgc cgcggacgtg ctgacggacc tcatgcaacg cgccgaaatc caccttgacc
     9961 agcatgggga tgatcatcac ccagatgagc aggccgacgg gcagattgac ctgcgccacc
    10021 tccaggcggc cgatggtccg gaacagcccg ggggcgagct ggccggcgac gatgccggcg
    10081 acgatgcaca ggcccaccca cacgctcaga tagcgctcga agacgctcat cggtgcgccg
    10141 gccgcggcct tggcacccac ttcacattga atgactgaca tggccgcccc tcacagaccc
    10201 aaagccagac ggacggccgg taccagacgg gcgcggatgt cttcgcgcac ggcgatgaaa
    10261 ctgtccagcg gctccccgtg gggatcatgg aagcccacgt ggatgcgcat caccgggcgc
    10321 gggaagatcg ggcagctctc cttggcgttg tcgcagacgc tgaccaccag atcgatgtcc
    10381 tggtccatga ctgcctcgac ggtcttggga tggagcccct cggtgggcag gccggcgagg
    10441 cgcagggctt cgagtgcgcc atccgccacc ttaggctgcg gcgcggtgcc ggcagagagg
    10501 gcgcgcacga caccggccaa ctcgtagttg aggatcgcct cgcccatctg ggagcggcag
    10561 gaattcccgg tgcacagcac gaggacggtt ttgagcaggt tcatcagggc gttccgtcga
    10621 aaagggtgat cgcgggatgc agggcctcgc agcgggcggg catgtcaggt gtcggcaggg
    10681 gccgcgcgac gcttggccgt cgtgtcgcac tgggcggtct tgggcaggca ggccttgccc
    10741 tcgcagcagt tgcgcgtcag gaaggcgatc aactcgtcca tcgtggcgta ctcggcggag
    10801 taatggatga agcggctctg acgctcgccc ttgatcagcc cgacgcggct cagatgcgac
    10861 agatggaagg acagggtcgc gccgggcacg ccccccatcc gctcggcaat ggcgctcacg
    10921 ttcaggcccg ccggccccgc ctcgacgagc aggcgaaaga tggccagacg ggattcgtgc
    10981 cccagggcag cgaggacttc ggcagcatca agcagttcca tatttctaat tatatggaaa
    11041 tattcgaggc cgtcaatccg acctgcgacc gcatccatgc gcgcactgcg cgccgttcgg
    11101 actttctgcg ggagaggcgg gtgctgcgcc gcgggacgcg tcaggccggc gtgagcccca
    11161 ggaccgccgc caggcgatcc agattctgtt cgttcgccgg aacgacgatg gcgcgcaagg
    11221 cctcggcgac ggccggatcg tcgaaagcct gcgcccagtg caggcgcgtg ccgcccgcct
    11281 cgggttcgaa ggtgagggtc agggtgaaat ggggggcgca ggtgtggcgg atcacgacgc
    11341 agcactccgg ctcgatgcgc gtgaattcgg cctggttggg ataggccgtc ccgtcgggcc
    11401 cgatcatcgt gaagtaccag cacccgccgg gctggaattc gaagcgctcg aaccgattgg
    11461 tgaagccgtc cgggccccac cagcgggcga ggcgctccgg atcccggaca gcggcgaaca
    11521 cctcggcagg ggtgcgggca aagtgtcgcg acgtgctgaa gcaggtcatg aatcgcgctt
    11581 tcaacccgcg ccggtccggc tcagcgggcg cacagggctt cgaactgccc cgccggcacc
    11641 gcacgggaga acaggtagcc ctgggcgtgg tcgcaaccga tctggcgcag caggttgcgt
    11701 tgctggtcgg tctcgacacc ttcggcgatg accttcaggc cgagcttgtg ggccatgacg
    11761 acgatggctt cgcacagggc gaagtcggcg ctgttggggt ccaggttgcg ggtgaaggac
    11821 tggtcgatct tgaggacgtc gatgtcgaac ttcttgaggt aggccagcga cgagtagccg
    11881 gtgccgaagt cgtcgatggc gacgccgacc cccaggtcgc gcaggcccag ccgcgtttcc
    11941 ttgatgccgg gatcggagct catcagcaga ccctcggtga tttcgatgac caggttgctg
    12001 ccgggcagct gggtggtctg cagcagttcg agccactggg tgggattgcg cccgttgaac
    12061 tgcaccggcg acatgttgag gctgacctgg aattccggcg ccagacgctc gcgccaccgg
    12121 ctggcctgct ggatgacctg ggcgaagacc cattcaccga tctcgtggat ggtgccggtg
    12181 tcctcggcga tggggatgaa ggtggcgggg ctgacgaagc cgtgggccgg gtggcgccag
    12241 cgcaacaggg cctcggcctt ggcgatgcgc ccggtgtcca ggtcgacgat gggctggtaa
    12301 tagacctcga gctgaccggc ctcgagggcg cggcgcaggt cgcccgcgag ccgcatgcgg
    12361 tgctcggcgc tctcctggag ggcgcgggtg aagaagcgga agcggctgcg cccggcgtcc
    12421 ttggccaggt acatggcctg gtcggcgtgc ttgatgagtt cgctgatggt ggcaccgtcg
    12481 cggggataga gggcgacgcc gatgctggcg gagacgaaat gttcctgcgc gttgaggacg
    12541 aaggcttcgg acaggcggtc gatgatgctc cgggcgacgc ggccgatgtc ggtgccttcg
    12601 gtgatgtccg acaggatgac ggtgaattcg tcgccgccca ggcgggccac ggtgtcgtag
    12661 tcgcgcacac agtggcggat gcggctggcg gcttccacca gcagcaggtc acccacctcg
    12721 tggccctggg tgtcgttgac ctccttgaag cggtccaggt cgatgaacag caggccgatc
    12781 accgaggcct tgcgctgggc cttcttgatc tcccgctcca tgcggtcgtt gaagaggcgg
    12841 cggttgggca ggcgggtgag gtggtcgtag ttggcctggg tccagatgag gttgtcggcg
    12901 cgtttctttt cggtgatgtc ggagaacagc acgacgcgcc ggttgacgct gccgtcgtcc
    12961 ttaaagacgg tgctgacggt gacccagccg atgaagtcgc gcccgtcgcg gcagtgcccg
    13021 ttgatctcgc cctgccagcg gccggtggcg tggatggcgg cccacatggc gcgatgggcg
    13081 tccacctcca tcacgtcggc gcgcaacacg aggggctcgc gcccggcggt gtcgtcctgg
    13141 gaatagccgg tgatctcgac gaaggcggga ttgacggtga ggacgcggtt gtccgcgtcg
    13201 gtgatcatca ccgcctcgga ggtgttggcg aacacggtgg cggccaggtg gagctgttcc
    13261 tcggcggcca cctgctgggt cacgtctcgg ttggtgccgc gggtgccgag gaaacgcccg
    13321 cggtcgtcga tgaccggctg gcagacgtgc tcgatccagc gttcggagcc gtcgcgggca
    13381 gcgatgcgga aattgggcag gcgatggccg ccggactcgg gcgagcgctc gttgtgggca
    13441 tcccagatgt agagatcgtc cggatggatc atcttgcgca tcagatccgg gtcggcgatg
    13501 aaatccaccg ccccgtagcc cgacacgtcg cggcaggccg gcgagacgta cagatagccg
    13561 ccagtgggtg cgacccagta ttcccagttg ggggagtact cggccaggat ctggtacttg
    13621 gccttgctct cggcgagggc atcgttggcc tcctgcagcg cgcccatgcg gtctacgacc
    13681 agcttctcga gggagcgctg gaggcggctg aggtccaggt gggtgcgcac gcgggcgcgc
    13741 acctcgtcga tgtcgaaggg tttggtgatg tagtcggcgg cgccgatgga gaagccctgc
    13801 accttgtcca ccacgctgtc caccacggtg acgaagatca ccgggatgcg gttgcccacc
    13861 ggcgtggcct tgatgcggcg gcacacctcg tagccgtcca tctggggcat caggatgtca
    13921 aggaggatca ggtcgggcgg attcaccccg gccaccagtt ccagcgcctt ggcgcccgtg
    13981 ggcgcaacca tgatgcggta gtcgtcctgc agcgcggcga cgagaccgtg gaggttctcg
    14041 ggcacatcgt ccaccagcag cagcgccggc ttgtcggagc gcgccatgac cgcggcgccg
    14101 aggccttcga cagccttctt gcgccggtgg gtgcgcaact ccagctgatt gcgcacgcgc
    14161 accaggagca gttcggggtt gatgggcttg gtgacgtagt cggccgcccc caggcgcagg
    14221 ccgacggcct cgtcggcggc atcggagagg ccggtgacga agatcaccgg gatgtcggcg
    14281 gtgcccggat cggacttgag gcggtgcagc acctcgtagc cgtccatgcc aggcatgcgg
    14341 atgtcgagca ggaccaggtc tggcggcggc gtgcggacgg caagttcgag cgccttcggt
    14401 ccattggtgg cgacaacgac ggcgaactca ccccgcagga tgttcatcag ggtgtgcaga
    14461 ttgtcgttga tgtcgtcgac aatcagaatg cgcggcttgg cttcgtcctg cgaagtcacg
    14521 gtggcgtcgc tccggttcgt ccttccagtt gcgtctgcag agcctgcagg agaccaagcg
    14581 cttcgtcgat ctcgaacaca tcgacccgtt ccgccacctc gcggaccgcg gcctccagcg
    14641 gcgaaccggc gatgccggcg agcaactcgg cgagcacgct ctgggctgcg cccacgtcgg
    14701 actccagcag cccccgcaga cgcccgacgc gggccagcac gtcggtatca gcaagaccgc
    14761 cgcccgcggg cgcgccggcc tgctcgggcg cagccagact gtcgatgtcg gccatcacgg
    14821 tggcgagcag ttccgccatg cgggcgaggc tggcctcatc gggccggatc tcctgcttga
    14881 gcagattgtc gatgtcggac acgcaggcaa acagcgcgtc ggcaccgata ttgccacaga
    14941 cgcccttgag cgcgtggcaa tactcctcgg cctggcgcgg agaatggtgt gcgaccaggt
    15001 cgcgcaggcg ttcggcggcc gtgtcgtagc gctgtcggaa gcgtcgcagc tgcttgcgat
    15061 aggcttcgac atcgccaccg atgcggcgga tgccctgctc ggcatcgagg ctgcgcaggg
    15121 ctcgcagatc acttcccggc gcgctggacg ggacaggccc tggcgccccg ccctccccca
    15181 gcggaatgtt gagccagcgg gacaaggccg cgcgcagacg ctccggcgac accggcttgg
    15241 tgatgtggtc gttcatgccg gccgccaggc tacgctgctc gtcgtgggcc atggcaaggg
    15301 cggtcatggc gatgatgggc agttcgccca ggtgttcgcc gccgggctgc tggcccaggg
    15361 cgcggatgcg ccggcaggct tccaggccgt ccatcaccgg catgtggatg tccatcagca
    15421 cggcggcgta gggggcacgc tggaccatgg ccacggcctc ttcgccattc accgcacatt
    15481 cgacctcggt gttcatgctg cgcagcagtt cggtggcgaa ttcccggttg atgtcattgt
    15541 cttccaccag aagcagccgc gcgccgcgga agtcgcaggc gcctccccgt tcggacctgc
    15601 tgctctcctt gccgaggata cgcccacggc cgagattgct gaggatggtg tcgaggaggg
    15661 tggacgggga cacgggtttg accaggaagc cggccactcc agcctgctcg gctgatttga
    15721 tgacgtcctc gcgaccgtag gcggtgacca tcaccacctt gggcttgtgg cggatgagcg
    15781 gcgaggcgtg gatgcgccgg gtgacctcgt caccgttcat cggcggcatg cgccagtcca
    15841 tcagcaccag gtcaacgggc gccgcgtccg gcacctgcag gcgttcgagc gcctcggcgc
    15901 cgctgcccgc cacgctcacg tccaggtgga agaaacgcag catctcggcc atgatctccc
    15961 gcgacacctc gttgtcgtcc accaccagga cacgcacgcc gtcaaggagc ggcccggcct
    16021 gctccaccag ccggcggcga tgggtctggg cgtcccgggc aatctgcagg tggatcgagc
    16081 agcacaccgt ggtgcccttg cctggcgcgg actgctcgat ccacaggcgc ccgcccatca
    16141 gctcggcaag gcgccggctg atggccagcc ccagcccggt gccgccgaag cgccgggtgg
    16201 tcgtctgatc ggcctgagtg aatttctgga acaggcgctc cgccacctcc ggcgcgatgc
    16261 cgatgccggt gtcgcgcacc gagaactgca gctccagatc cgtctcggtc atcgccacgc
    16321 agcggaaccc cagctcgacc tcgccctgct cggtgaactt gatagcattg ctgcacaggt
    16381 tgagcatgat ctggccgagg cggagcgggt cgcccagcag cgcgggggga atattgacgt
    16441 cgtagcggat gaggaactcc aggcccttct gctccgcctg caggccgaca gaatccttga
    16501 gctgctccag caccgtgtcg aggccgaact cgatggactc gatctccagc ttgccggcct
    16561 cgatcttgga aaaatccagg atgtcgttga tgatcccgag gagggaatgg gcggccccct
    16621 gagccttggc cagatagttg cgcagggccg ccggcggttc gttcttgagg gccaggtaga
    16681 gcatgccgat gatggcattc atcggcgtgc ggatttcgtg gctcatggtg gcgaggaagt
    16741 cggacttcat gcgcgacgct tcctcggcca tggtcttggc cttgatcatc tcgtccatgg
    16801 cgtcccgctc ggccgtgatg tcctcgatca cgcacacggc accccgcgcc ggatcggccc
    16861 ggtcgatgac acgggcggac aggcggatcc agaatgcgct gccgtccttg tgccgcgcca
    16921 tgagttcgcg gcgcacggtg tcgccagccg ccagacgggc ggccgccaga gcctcggcct
    16981 cctcgtccac ggtgtcggcc aggcgatccc gcaggggccg cccggtctgc tcgcccggcg
    17041 cgtaaccgaa catctcgtcg agacgccggt tggaacgcac gatgaggccg tccttgagca
    17101 gcacgatgcc cacgctggcc gagtcggaca gggcctgcag ctcggccgtg cgctcccgca
    17161 cccgctgctc cagcgcctcg ttgacggact tgagctgggc ctcgttttcg gacagccggc
    17221 gattggcatc cagcagcacg gtgtcccggt cctcgatggc agcgagcatg ccgttgaaga
    17281 cctccaccag ggtgccgatc tcgtcgtcgc tgtccagcgt ggcccgcacg ctgtagtcct
    17341 tgctgatggc caccatctgc accgtgcgcg tgagacgttc gatgggccgc gacacgatgc
    17401 gctgccagcg cgtcgccacc agcagggtga tcatggacgt gacaaaggcg gtgagcgccg
    17461 caaagagcag gtaatcccgc cacagcaggt cgagctcgcg gaagctggcg cggatgaaca
    17521 cggcgccctg gggcgtgcct tcgacaagga tgggctcgat caggtgcagg taaccaccct
    17581 cggtgtacga gcggctctcg aacttgggat cagcgggaaa gctgaagggt cggtcctcac
    17641 cccgctcgta gcgggcaaac acccgtcccc ccacgtcgta gatgcaggcg gcgctgacag
    17701 cccgcttgat ccgcagggcg cccagggttt ccatggcgac cttgtcgtcg tagaacagca
    17761 gggccgcgga gctgcggtcg gccacgatgc gcgccaggga cgacaggtcg cggaccagtt
    17821 cgtcggtcac ccgcacccgc tcgtaggcga tgaagccgac cgcttcgatg agcagcgccg
    17881 ccacgctggt gatcacgatg atcagcacca gcttggcctt gatctgcatg ccttcaaaca
    17941 atgcgcgcat ggtcatctgc cgggttcggg ggaaagatcc cggcgcacgc cgccaggcac
    18001 ggcgcgacag gatgcggggc gtgatgcggg cggaccatct ttcagcggtc ctgcggcacg
    18061 gtgcgcgcca cctcgagcag cttggcgctg atccgcaggt tggcgttgcg ggcggcgtcc
    18121 ggattgatct caagacggat gcggccaccg tcggaataaa agcccaccac cccaccgatc
    18181 cgggcgaaac cgtcctggtc gctgatggtg agcacactcc cgccgcgcag gcgcgcctgc
    18241 aggtcggccc agcgccgctc gccccggcca acgaagagca cctggcactg ggccggatcg
    18301 aaaccgttgt cgatgtccac gcgcagcgga cggtccttga cctgcttgct ggccagttcc
    18361 cccagcagga cgcccaccgg atcgctgccg ctcacgcata gacgcagggc gtccgtcggc
    18421 agcacgggcc aatccacaaa tttgatgacg tggaagacgt aggccgcctt gatcttggct
    18481 tcaagctccc ccggcgccga aaacgccatc aggggcaggg ctgcggcgca caccagcagg
    18541 cggcgcaaca gcgggacaag ccggaaccgg cggcggacgg acgcgcgcat cgcccgcggc
    18601 cgcgacgcct caataccgga cgtcgacgcc ggcaaagcca ttgaaaccgg gatcgcggaa
    18661 atcgaagggc atggcatggt gccggtcggt caggttgttc aggtcgacgg tcagatgaac
    18721 cgccgcgcgg gcgtcacggt acaggggctt ctgcacacgg acgttcacca cgccgtactg
    18781 gccgaaaccg agacgggcgc tgcgggacgt actgtcatag tagcgcccga cccaatgata
    18841 ataggtcgcg acactcatgt cgcccggcaa actggccagc acccccgcat tggcgagggt
    18901 gtccggcgcg aaggggatgg cggtcccgtc atgatccgcg ttggtgggat cggtcacccg
    18961 ggtgtgggta cgggtgaggt tgccgaacac actggtttcg ctcgacgctg cgtagcggag
    19021 atccacctcg tagcccatgg cgcgggcctt gcctgcgttt tctgaacgta cctgggacgg
    19081 cgccgcgctg acgatgttgg tgacgatcgc gtcgttgatc gtgttcagga acccccgcag
    19141 atcgagggtg agcgcctggc tggcgcgcca gttgacgccc acgtcacgac cgacgcccgt
    19201 ttcgggcttg agaccgggat tgggcagctc gccgctggcg gtgggcaccg ccacggtgcc
    19261 accgatctgc ttggccgcgg gcgccatgaa actggtgccc acattgccgt agaaggccag
    19321 accgggatcg gcgttgtagc gcgtgccgaa gctccacaga ttcttgctcc acgcggcgct
    19381 acgcgtggcg ggcgtgatgc cgccgagcag gtcgtaatcg tgatggatgg tgtcgtgccg
    19441 aactcctcca cgcagcaccc aggcatccca caccagcttc tcctgcacga aataaccagt
    19501 cgagcgggcc tgggcgcggt tcgacggggt gacggccccc acgggatcct ggacgtcggt
    19561 ctgataatcc acccactggc cgtccacccc ggccgtcagc agcgactcgc cccagtggcg
    19621 atggctcagg gtcaggtcgg cggggcggat ggtctgacgg gtgaattcgt ggttggagag
    19681 gctcaggtcc gtcgggtagt tgtcgttggc gaagcgccgg tcatagcgcc gttcgccata
    19741 cttgaactgg atgttccagt cgtcgctgaa ggtgttgctg taggcgaagt tgagggtgtc
    19801 gtaggtgtgg tcgaagcccc ggttcggacg gcccatcgtg ccctggtgca gggtgcgttg
    19861 atagaacacc gtcaccgtat gatcgggccg gtcgaaggcg tagctgaaat tgccgaagaa
    19921 tcggttcttc tggtaggccg gcgcctgggt ggtctgcagc caggaattgc gggtgccgta
    19981 ctgggtgtaa tcggaatgct ccaggttggc actgacgatg tagcccagat tgccctgcgt
    20041 cccctgcgcg taggccttgc cgaacaccgt gccccaggac ccggcgccgg cagcgacgcg
    20101 ggtcaggggc gtatcgaccc ggctcttcag cacgaagttg gtggtcccgg ccaggggcgt
    20161 ctcattgccg acgaaatcca tggtcaggta attggagtac agcacggacg ccggcccctt
    20221 gtgcacctcg acgcgctcga tggcggaggg gtcgaggctc atggcatcca cgaaggaatc
    20281 gatgggcagc ccgtccagca gatagctgtt gtacttggga ccgagacccg cgtaggagcc
    20341 ccagttggca tcgttgcggg acagcacatt gacgaactga ccggacgtgg gacgcatgag
    20401 ctccgccaga ttgccggagt gcgcgggctg gcgctcgatg tcttcgttgc gcagcacgac
    20461 gatgcgctgc gtgacggtat ccgccgcctg ggcgagcttg gtgtcggtca ccaccaggtt
    20521 gcccagctgt tccaggctca actggtccac gaagccgctg aggggcagat tgcggatctc
    20581 gccgggatcc gccggcgcat cggcaagact gggcgcggag atcaggacga aggtggaggc
    20641 gagatacgct tttttcatga tttcagcaat aaccccgaac tttaaagggt attttcgcgg
    20701 aaacaaagat ttacaatacc cttcgttgca tggtatcccg ccgtccggcg aatttcaacg
    20761 aaaccattgg catgtcagcg ggcctgaaat ccgcgtcacg ccgtactgcc gggcgttccc
    20821 ggcgcgtcgc gcagatgcgc tgagagatcc gcccgcaccc agtccacaaa ggcccgcaca
    20881 cgcaagggta ggaggcgtgc atggggcacc accacgctga ccgggcgcgg cgcctgctcg
    20941 aaggcttcca gcaccggcac cagccgcccg gccgccagat gaggggccac ctggtaggag
    21001 atgaacatgc cgaagccaag cccggcgatg caggcatcca gggccggggc gacgtggttg
    21061 aactccaggt tgccgcgcac cggcacgccg aaactgcgcc cctgctcctc gaaacgccac
    21121 cacacgcccg gcgtgggcga aaagccgatg caattggccc gcagcaagtc ccgcgggtgg
    21181 gccggcacgc cctgacgagc caggaagtcc ggactcgcca ccaccatgcg ccgcacatgg
    21241 ccgacgggat gggcgacgag ggtcgagtcc tccagcacgc cgatgcggat gcccacatcc
    21301 acgccctcct ccagcaggtt cacgatccgg tcgaagagca gcacccggca atgtacgcgg
    21361 ctgaagcgct gcacgaagcg ggtgatggcc ggtgccacga aaagttgccc gaacagcacc
    21421 ggcgccgtca ccgtcaacgc gcctgacggc tcgccccctt cgtcccgcag cccggattcc
    21481 gcctcgtcca gtgccgccag caggttgcgg caactgtcca ggtaacgccg cccctcctcc
    21541 gtgagggcga tgcgccgtgt actgcggttg aacaggcgca cccccaggtc cgcctccagc
    21601 gccgccagcg cccgcaccac cgccggcagg gacatgtcca gcacccgtgc ggcgccgctc
    21661 aggctgccct catcggcaat gcggacgaag gttccgatgg ccttcagctt atccatggat
    21721 tactccgatt tccggatcag ttaaatccgt tttaagccat ttattcacat gccggattaa
    21781 accacactgc cggacatccc tcccccgcgc cgaaaggacc gccatgaaca cccccgccca
    21841 acccatcagg ttccaccgct tcgccacctc cggccacagc caccgggccg agctgatgct
    21901 gtccctgctc ggtctgccgg tcgaacccat cgacgtggac ctggccggcg gcgcccagaa
    21961 gaccccggcc tacctggcca tgaactgctt cgggcaggtg ccggtgatcc aggatggcga
    22021 cgtgaccgtg gccgactcca acgccatcct ggtctacctg gccagcgtct atgacgacgg
    22081 tcgctggctg ccccgcgatc cggccgccgc cgcgcaggtg cagcgctggc tctcggtggc
    22141 cgccgggcag ctcgcctacg ggccgtgcgc ggcccgcctc gtcaccgtgt tcggcgcccg
    22201 cttcaacgcc gacgaggtca tcgcccgcgc ccacgccctg ttcaagatcc tggatgcgga
    22261 gctggccacg cgcgcctacc tgacgggcga cgcgcccacc atcgccgacg tggcgctcta
    22321 cagctacacc gcccacgcgc cggaaggcaa cgtcagcctc gacccctacc cccacatccg
    22381 cgcctggctc gcccgcatcg aagccctgcc gggtttcgtg cccatgccgg ccaccgcggc
    22441 cggactccgg gcctgagcca tcatgaccgc gcagacgccg ccgcgatccg tcttccacgc
    22501 cggcgaggag gccctgcagg cccttgccgg cgcgcaggcg cgattggccg aaacggggcc
    22561 gcgggtgatc cgtgaggtgc tgcccgagca acaccgcagc ttcttcgccg aactgccttt
    22621 cgcagtgctc ggcgccctgg acgcggacgg ccagccctgg gccacggcga tcgccggccc
    22681 ccccggcttc atcaccagcc cgacaccgga gcgcctgcgc atcggagccc ttccggcggc
    22741 ggacgacccg gccgccgccg gtctcgcccc cggtgcggca atcggcctgc ttggcctgca
    22801 gccccacacc cgccgtcgca accgggccaa cggccgcgtc gcagcgctgg acgcggacgg
    22861 cttcaccctg gctgtcgaac agagcttcgg caactgcccc aagtacatcc acgcccgccg
    22921 ggttgaacac gcggcgcaga cggctgcccc ggcgcccgcc cgcgtccagg cccgactcga
    22981 cggcgcgacc gccgacctga tccgccacgc cgacaccttc ttcatcgcca ccgcccatcc
    23041 cgatgccgca cggggggaac acgccgccca cggcgtggat gtctcccacc ggggcggccg
    23101 gcccggcttc gtgggggtgg acggcgacca cctgtgggtg cccgacttcg ccggcaacca
    23161 gtacttcaac accctcggca acctgctcct gaatccccgc gccggactgc tcttcgtgaa
    23221 tttctcggag ggcagcctgg tgtggatcgc cgccaccgcg cagatcctcc acgacggtcg
    23281 cgccgtggat gcgtatcccg gcgcccagcg cgtgctccag ctgaccgtgc acgcgctgtg
    23341 gacggccagg cggcgcctgc ccctgcacag ccaggacggt gaggcatctc ccttccttgc
    23401 cggcacgggc atctggcccg gctgacgcct gccccttgac catgccccgg ccgccggtgc
    23461 gaagatgaga tccgtcgcct tcgcctcgcc ggccccatgc ctgtccatca cgacgccttc
    23521 atttcctaca gccacacggc ggacggcgcc ctggcgccgg ccttccagcg cggcctggaa
    23581 cagctcgcca aacccctgct gcgcctgcgc gcccagaaga tcttccgcga ccagaccgac
    23641 ctcaccgcca gcccggccct gtggcccggc atcgtcagcc acctggccgc gtccgactgg
    23701 ctgctggtct tcgccaatcc gctctacgcc ggctcccgct ggtgccgcaa ggaggtggag
    23761 tggtggctgg cggaacgcag tccggaacgc ctgctgatcg tcctgaccga cggagacatc
    23821 gtctgggacg aggcacgccg cgacttcgac tgggcacgca ccaccgcgct gcccgactgc
    23881 cttgccggcc atctggcgga cgaaccgctc tacgtggatc tgcgctgggc gcgggagacg
    23941 ccgggcctgt ccctcgccca cggcggcttc cgcgaagccg tcgtgcgggt cgccgcgccg
    24001 ctgcgcggcg tcagcccgac cgatctggac ggcgccgatc tgcgccagtt gcgccgcacc
    24061 cgcctgttcg tgcggaccgg cgtgggcgcc atctgtgtcg ccgccggagt ggccgtctgg
    24121 caggcccgcg tcgccaccga agaggcccgc attgctgaaa cccagcgcca gcaggcagag
    24181 gcccaacgcc gcatcgccga atccgaacgg gcccaggccg aggcccagcg nnnnnnnnnn
    24241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcccagcgcc acatcgccga gtccgagcga
    24361 gcccgcgccg aagccgccct cgccgcaagc gaacgggagc tgttgcgcgc ccagtcggcc
    24421 gaactgcggg ccctgcgcgc acgcctcgac acgctcatcg ccgacgcgat cacaccaggc
    24481 cacctgaccg agatcgcgcc catgcaggcc gagcgcgcgc tgctcgacca gcgcctcgcc
    24541 gatgtcaccc gtcagtacct cgccaggctg gccgagcaga tcggttatcg cggcggcttc
    24601 gagttcctga tgaagtggga gggcaacgcg gggcgggtcg cgctgatcgg ctcatcggtc
    24661 ttcatcgacc ccctcaccga cctggcgctg gcaaagcccg acgatctgcg ccgccgctac
    24721 gaatttctcc tgacgcccgc ggagctccag gccctcatcg ccaccgccgg caaacgcggc
    24781 gccgaggccc aggccgccta cgccgccgcc ccgatcctgg cacgcatccg catccgcgcg
    24841 gaggacgtgg cacgcctggt gccggacgtg atcgagcctt tctggaagcg cctgcgggac
    24901 aagcatccca tcctcaggcg cccggacacg ccacccgcgg tccatacggc cctgctgtcg
    24961 gtggcggtca atctgggcgc acgggtcgaa tccctcgcgc ccttcatcga cgctggccgc
    25021 tggcgcgaac tcgccgacgc catcgatgcc ctcgctgcgc gcccgccagt gctggcaggc
    25081 ctcaaacggc gccgcgccga ggaggcggcc ctgatccgcg ccgccctggc ggggcaaccg
    25141 ccataaggcg ggtcagaaca ggtggctcac agcctgcgcg caccacacgc cggcgcccag
    25201 cacgaccagg ctgcaaccgg cgaaaaagcg ggccggatgg tcgagagcgt tggcgcgggc
    25261 gtcccgatgg acaatgcgga gggtggtcat ggcagtctcc cggtgaaatg gatggcgtgg
    25321 tgtccatttt ccggaaggcc cggcgggcag gccaatgaga tcttccaacc accgggatag
    25381 gcccgggcca tcgcccggcg cggcccccgt cccgacggtc ggacttactt gagccccgtc
    25441 acggccatct tgcgccgcag gaaggcgatc tgggtctgga gcgacaggcc cttcgggcag
    25501 acatcgtggc aaccgagcag cgacatgcag ccgaacacgc cggtgtcgtc gccgatcagc
    25561 tcgtagaaat cgtcgtcgct gcgttcgtcc cgtggatcga ggcggaagcg ggcgatcttg
    25621 ttgagcccca ccgcgccgac gaagtcctcg cgcatgcgca gggtgccaca ggcggccacg
    25681 cagcagccgc attcgatgca gcggtcgagc tcatagatcg cttcggcgag ggccggctcc
    25741 atgggcgcct cgacggccgt gagatcggcc tcggccgtcg tgtggatcca ggtttcgagg
    25801 cgttcggcgg tgccgcgcat ccacttgccg gtattcaccg acagatcgcc gatcagctcg
    25861 aagaccggca gcggggcgag ggtgatcagc gggtccagat cccgcgtcag gctgcgacag
    25921 gccagcccgg ggcggccgtt gatgagcatg gcgcagctgc cgcagatgcc ggcgcggcac
    25981 acgaagtcga actgcaggga tgggtcctga tgcgcgcgca cctccgtgag ggcgatgaag
    26041 agggtcatgc cctccgtctc ctcgatgcgg aagtcctgcc agtgggggtc gatgtccggc
    26101 ccgcagcgca ggatgcgcag cgtcagggtg cgcgggcccg cggacgcgcg gggcttcatt
    26161 gtccgtggcc ccggcgcacg tcgtggcgct gctcgtccca gccggccgaa tgcacgccgg
    26221 gcgccacggt gaattcgtcc gggaggtccc gcacgaaggt ggtgcacggc aggcagcgct
    26281 ccccattcac cagcacgtag caggtggcac aggcgcctgc atggcacacg aaggcatcgc
    26341 cgaggggggc gggcagggac gggcggatgt ggttgagggc gttgaggagg gtcagtcctt
    26401 cggtgtccag cagggcgtat tcgtcggggt ggtctgccag gcgcagggtc acgatgcggg
    26461 ttggggtcgg ggcgttcatg tgtgttctcc tgaacggtgg tttcggccgc ggaagcgcgt
    26521 cggaaccaga tgttcgtacg gcatcagggc ctgctgtacg gtgtagcggt cggccgttgc
    26581 gaaggcgctg cggatcgtcg cgatctcgtc cgcacggcgc gcggtgtcgg gatgctccac
    26641 ggcattgcgg gcgccgtagc cgcgccagcc aggcggcagc tccatgcgca tcacgtcgag
    26701 ggcctcatag ctcagggtcg gccgggtctg ctgcgggtcg ggccaggtgg ccagggtgcg
    26761 cttgagccac agggcgtcgt cgcggcgggg gaagtcctcg cggaaatggg cgccacggct
    26821 ttccgtgcgt tgcagcgctc catcggccac gcagagggcc agcttgagca tcttctgcac
    26881 ccggtaggcg gtcacgagtt ccgggttggc ccccgggcga cgcgtggcga tgccgatacg
    26941 gcggctgcgc tcgagcaggg acgccagttc ggcgacagcg ctttcgagca ggtcgccccg
    27001 ccggaagatg ccgacctgct cggtcatgat ggcctgcatg cgggcgagca gcgcctgggc
    27061 gtcttcggtg ccagtgccgc tcagcagcgt gtccagatga tgctgtgtcc gctcgagaca
    27121 gtcgcggacc agcgcggtgg agacctgcag ctcacccatc ggcgattcca cgaaatcggc
    27181 catccgctcg ccgacgatca tgccggccac caccgtctcg gcgacggaat tgccgcccag
    27241 acggttgaac ccatggaggt cccagcaggc cgcctcacca cacgcgaaca ggccgcgcag
    27301 gccacggacg tgcccgctgg cgtccgtgcg gatgcccccc atggaatagt gctgggcggg
    27361 acggaccggg atccagtcgc gggacgggtc gatgcccagg aaatgctggc agatctcctt
    27421 cacctcccgc agacgtccgt cgatatgggc cgcgccgagg agcgtgatgt ccagccacag
    27481 gtggtcgcca tagcgcgtct tgaccccgtg gccggcacgg atgtgctcct ccatgcgccg
    27541 tgacaccacg tcgcgggaag cgagctcctt cttctcgggc tcgtagtccg gcatgaagcg
    27601 gtgacccgcg gcatcccgca gcagaccgcc atcgccacga cagccctcgg tgacgaggat
    27661 gcccgccggg aagatggtgg tgggatgaaa ctgcaccgcc tccatgttgg ccagggtcgc
    27721 cacaccggtc tcaagggcga tggctgcgcc gatgccctcg ttgatgatcg cgttggagct
    27781 gacctggtag atccgcccga agccgccgct ggcgatgcag gtggccttgg ccacatacgc
    27841 gctcagggcc ccggtgacga gatcgcgcac gatggcgccg tgacagcggc cgccttcgtg
    27901 gatcagggcc agcgcctcgg tccgttcatg gaccgggatg cccgccgcga cggcctggtc
    27961 gcccaccgcg tagagcatgg cgtggccggt gccgtcnnnn nnnnnnnnnn nnnnnnnnnn
    28021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnnctgg cctgcatgcc gccctgggcc gccgccgagt gggaacgctt
    28141 gggcggcacc agactcagca ggatcacttc gtgcccgcgt cggcgcgcgc cgaccgcggc
    28201 ccgcaggccg gccagtccgc caccgatcac cagcacgtcg gtatagacga tgcgcatgat
    28261 tactccatgg acggcgggtt cggcgccgcc gccagggcgg acggattcgg caccagggtg
    28321 gcgtgggcac ccggcacata gcgttcaccg gcccggtcgg cgtgggtcac gccgatctgc
    28381 acataggcgc gcagggtgat cagcccaagg atcaggaaga aggcactgaa gaccgtcttg
    28441 gccacgcgca agcggcgccg gctgactgcc gcatcgcgcc cttcgagcca gccccatttc
    28501 accgcgaggc ggtacaggcc gatgctgcca tggatctcca ccgcaaacag gagcaacagg
    28561 tacaggggcc acagggtgcc ggtccagatc cggtccgcgg attcgtaggg accgatcagc
    28621 tcgggctggg tgagcatgcc gtagaggtgc accgtcccca gaaagaacag ggcgaagccg
    28681 gtccacagct ggatccacca cagccccgtg tcgtcatggc gcaggcctgc ccgatgggcg
    28741 gcaaaggcgc ggtactgacg gaaattggcc ggaaacttgc gcaatgccag ccaggcgtgc
    28801 cccacgaaga ccagcaggac gaagccgacg acgcaggaca ccagccaggg ctgcggccgg
    28861 ccaagcagcc aggcgccctc gaagaaacgc gccacggtat aaaacgcgtc cttgctgatc
    28921 aggatggacg agacgaagaa catgtgggcg atcaggaaga ccgcgaggaa gaggccggag
    28981 acgctctgca gcacatccag gcgggcgggc cagcggctgg cacggctgcg gacgctgcca
    29041 cgatcggtcg gggcgaccac ccggggggcg gcactcaggg attccgcatc ggcggtacga
    29101 acggcggtag gagccatggc gcgaagacct ccagcttgct gccctccgca tgggagggct
    29161 gattccggtc ccgcatcgtg gcggccccgt cacgggcgtg acggcggcaa ccacggcaag
    29221 gggccgcagc ctgtgtctcc tgcactcttt ttatgggtag tggcgcactg caacgctctt
    29281 ggggtgttgg cactgtagca cagccttttg cggtgcaaca taggcctttt tcggggtctg
    29341 gacagtgaat ggcgccacgg cccgcccgaa tattcaggcg gaatcgtgaa atctgacccc
    29401 tcgtgccgag cggggacggg gaggcctact ccagccgcgc gggccggacg cagccgtcgg
    29461 gcagtccgtc gatttcgatc tggatggtgg gatggccgat ccggaatcgg tgcacgagtc
    29521 cgtcggccag ggcgatgagg aaggcgtccc ccgggtgacc ggcgggcatc accagatgcg
    29581 ccgtgagcgc cacatccgag gtccccagcg cccacacgtg gaggtcatga acgctgcgca
    29641 cgcctggctg gctgcgcagc caggcatgca cctcgaccag atcgaccccc tccggcaccc
    29701 cgtcgaaggc gagatgcagg gactggcgga gcagacccca ggtgcccgcc acgatcaggg
    29761 ccgcgatggc gaggctgacc agcggatcca gccaggccca gccaaaagcc aggtacaacg
    29821 cccccgcgcc cacgacgccg agcgacacca gggcgtccgc tgccatgtgc aggaaggctc
    29881 cgcggatgtt cagatcgtcc tggctgcctt tcatgagcag agcggcgctc agaccgttga
    29941 tcaggacccc gagggccgcc actgccatca ccgtgacggc gtcgcccgga tggggcgcgc
    30001 cgaggcgttg cacggcctcc agtgccagcg cccccatggc gaccaggagc agaatcgcat
    30061 tggccagggc cgccaggatg gaggcccgtt gccagccgta ggtgcgcaca tgggtgggac
    30121 gcaggcgcgc cgcgaaggcg gcgccccagg ccagcgccag gcccgccacg tccgacaggt
    30181 tgtgccccgc gtccgccagc agcgccaggg aatccgcccg ccagccgaac caggcctcca
    30241 cggccacgaa gagcacgttc agcacgatgc ccaccgcaaa agcccggtcg tgatgcgcgg
    30301 ggccctgccc atgcgcgcca tgggcgtgtc cgtggtgatg cccgtgccgg cagcccgggc
    30361 cgtgaacgtg actcatggct tcagcagcag ttcgtccagg cccttgcgca gggaacggga
    30421 gcggggcgga tcggacaccc ccacccggca gaagaaactc agggcctgat cggggccgat
    30481 ggccgcgatc tgctcgaagc gctccgccag ggccgccgcg ccacgatcga tggcggggtc
    30541 gtgggagatc ctctgcccgc tgcgggcata ccaccggaag atgaccggcg tcatctcggg
    30601 ctggagatgc aggccgaccg cagtggcggt gagccacaga cgctgcatgg ccgcgccggc
    30661 ccgcacgtgg tccgcgaggc cgcgcggcgg cacgtcggcc ttcagcagga ggtgggccga
    30721 gcaggccagc gcgggaatca ggtcgagctg cacccgcggc gcgatcgtac cgccgaggaa
    30781 acgattgaag aactgcaccc gcccccagct ggccatgacc cattccatca ggcgtgccgt
    30841 cgcggcatcc acccccacgg cctgctccgg aatgcggtcc ttgctgaagc gggcccgcca
    30901 ctcgatgacg tccttgtgca ccgggtaggc ttccgggcag gtcaggcgca gatacgcatt
    30961 gcgccacagc aagcccgcca cggcacgccg ttccgcgccc gtctcgaagc actgcacggt
    31021 gtagcccgcg cccggagcgg cggcgagcgc cgtccgctgg cccgaatcaa gcggcgtcgc
    31081 acgcatggcc cggcgctgca cggtgcgccg ctcgatgcag gacagcagcg ggtcggctgc
    31141 cagcccggag accggcgaga gggtcacatc atagaccggc gcggtgtcgg tgctgtccgc
    31201 ccgcaggcgc cagacggcct cgagcccgtg ggccgtggcg gcgatgcgca gcgtctccag
    31261 gagcgcaccg tgggccatgt ggctggcgtg gccgttgtag tcatacaggc accaggtgcg
    31321 cgtatcgtga ccgtggacga caaggcggtc gtcaccggcg atctcgaagc gccagggctg
    31381 ggtgttgtcg ccactgggcg cccagcgcgc caggtcgagg atctgcaggc gggtgtcgcg
    31441 ggggatcatg gcagcaaccc cgcccgatcg gcaccgggtt ggcaggcagg cgagcctgcg
    31501 caaacgacag gagcgtgggg acgtttcacc tggatgacct ccctcgaaac ggttcagcgg
    31561 cgcatggcgc gcagctgacg gcgcgcgatg aacagggcga ggcgctgcaa tggattacgg
    31621 ttgccgcctg gccgccaggt cttgacgtag cgattgcgat aggcgtcgaa ctggaagccc
    31681 cgtggcgcgc acagcacgtc accgcgcccg agcaggatct tgagggattc aaccgcggtg
    31741 acgccggcgc acagctggca cgcggcaatc gtggatggcc cgcgctgttc ggccagattg
    31801 acccgcgacg ggtcggccag atagccgcgc tggagcatgg ccggcgagag gccgagcagg
    31861 aagcggatcg ccatctcctc ctcgtcacag ccgtcgaggc agaaataatc ctcgaaggac
    31921 atctggccgg gcaggaagct caataccgcc gttcccatgc cgaggggggc cgcggtgacc
    31981 gccggaatcc cccgcgccgc gcaggcgcgg aaggtggcgc ggcgggcatc gaaggcgaag
    32041 aagtcgagcc cgtccacgta cagatcgaca ccgtcgagga aatcgcccag attggcgtca
    32101 tccacgcccc gctcgaagag gcggatgtcc agttcgggat tgatgtcgcg ggccatctcc
    32161 gccagcgccg cggccttggg gcgctgcagg ttggacaccg tggcccccac ctggcggttg
    32221 aaattgacca gctcgaagac atccatgtcg gcaatgtgga aggcaccgat gccgagccgc
    32281 gcaagggtga gcagatgcac gccgccgacg ccgcccatgc cggcaattgc cacccgcttg
    32341 cctcgcagag cggcctgttc ggccaccgtc acccagccga tattgcggga gaaagcctcg
    32401 tcgtaaacga actggaccat ggaaatgccc ggtttgcctt gccggcggaa caggtatgat
    32461 acgacagttc atgccgccat ccttcgcggt gcgccgcgcc cctgctaata ttccggcggg
    32521 attgcggcga agccggcgcc gtcatccggg cacggggccc cgatggccac acgcccgcgt
    32581 gcctgcccgg acctgctctc gagaccatgc cgttcacact tgcgatccgc aacatcctgc
    32641 gccagaagtt ccgtaccggg atgaccctcg ccgcgatcat cttcggggtc gtcgggctga
    32701 tcctggcggg cggtttcgtg gaagacatct tcattcagct gggtgaaacc atcatccact
    32761 cccagacggg ccatatccag gtcttcaaga aggaattcct tgaaaagggc atccgctatc
    32821 ccgagcgcta cctgatcgat catcccgaag acatcgcccg ccacttcgac gggcatcccg
    32881 aggtggaagc cgtgatgagc cgggtctatt tctcgggtct cctcaacaac ggccggcgcg
    32941 acctgcccat catcggcgag ggcgtcgagc cggacaagga aaccaagctc ggcagtctgc
    33001 tcaagatcac ggaaggtcag cccctcacgg acaaggaccc ggacggcatc ctcatcggac
    33061 agggcgtggc ccagtccctt gcggtcaaac cgggcgacgc cctgaccctc gtgaccagtt
    33121 cggccggggg cgccatgaac acccacgact tccgcgtcgt tggcatcttc cagagtttct
    33181 cgaaggaatt cgacgcccgt gccgtgcgca ttccgctgaa tgccgggcag gatctgctgc
    33241 agacccgcgg cgtcaatcag gtggtcgtcg ccctgcaccg caccgacgac acggatgccg
    33301 tcctgaaacg cgccagcgcc gagctcgcgg acagccctta cgacgtccgc agctggcgca
    33361 ccctgtcgga tttctacgac aagtcggtcc agctctacga cacccagttc ggcgtgctgc
    33421 ggctgatcat cctgctgatg gtgctgctgt ccgtcaccaa cagcgtcaac atgaccgtct
    33481 acgagcgcca gggcgaattc ggcacgatgc aggccctggg caatcgggcc cgggacgtcg
    33541 tccgcctcgt cgtgctcgag tcggccctgg tgggcctggg cggcgccctg gccggcgtgg
    33601 tgctgggtgt catcgcagcg ctgatcatct cggccattgg aatcccgatg ccgccgccgc
    33661 ccaatgccaa cgtgggctac accgcattca tccgggtggt gccgggtacc ctcgccggcg
    33721 ccttcctggt cggctttgtc gcgacggtgc tggcctccct gttccccgcc tacaaggtct
    33781 cccgcctgcc ggtggtggat gccctgcggc agaacgtctg acctctttcg atccgagtac
    33841 gcagccatgg ccttcttcga caacctgacg ctgaacctgg gcgacggatt ccgcaagtat
    33901 ttccagatca gcgcggcctt cgacaacgct ggccgcgacg acgttttcgc cattcgccac
    33961 gaggtgtact gcgaggaact gggcttcgag cccgtccgcg ccaatcggcg cgagaccgac
    34021 gagtacgacc gccacagccg ccattgcctg ctgcgcacct cgaccgaacc ccacaccctg
    34081 gtcgggtgca accgcctcgt cctggcacga ccggaggatc cggcctaccc gctgcccttc
    34141 gagcgcacct gcgcggccac cctcgatcgc cgcatcatcg acccggccaa ggagccccgg
    34201 ggcgccatcg ccgaggtctc acgtctcgca gtgcgcggcc tgtaccgccg tcgtaaaggg
    34261 gaagcacgca gcgaagtcgc cctcagcgac agcgacttcg gcaccgccgc ccagccgcgt
    34321 ttcccctaca tccccatcag cctgtatctc gcggtggtgg ccatcgccca gcgggaaggc
    34381 gtcgagaccc tcttcgtgct caccgaaccg cgcctcgccg atcacttctc caaactcggc
    34441 gtcaagatcc gccagatcgg cggtccggtc gaacaccgtg gcacgcgcat tccatccatg
    34501 atggacgtga agggcatcat cggcggactg cgctttttca tgaaacccat gtggcgggtg
    34561 gtgaacgaac agatcgacgc gggctacacg tccggcagcc ccgacctgcc agccggcagc
    34621 gcctgctgac gcgtctgcgc ccgcgacatc gcgcgggatg cgtacgattt gtcaccccga
    34681 tgcaaccgcg aggcgttatg ctgcacagca caatcgccac acggagtccg ccatgtacct
    34741 ctcccccgat gaaatccacc agaaccgcct ggacaccctg cacgccatcg aggccgcatc
    34801 ccaggcctgg atcgacgcca ccggcaagct ctccgagctg atcctgcggg ccggccgtca
    34861 gatgctcgac gaaagcgagc gccatctgag tcagctcgcc caaccgggcg ccagcctacc
    34921 cctgtccatc ggcagcctcg atgcctggcg cagcgactcc gccgaactcg tgcgcgacag
    34981 ctttgccatc atcgcccagg cacaccagac caccctgcag atcgcccgca agcaggtcgg
    35041 catcttcgac ctcgccctca agcgcggaat ggcccacgcc attcaacatg cggacgcaga
    35101 aaccggcagc gccatcagcc acgtgcgcac ggccctcgaa caggccgaga cctgcctgaa
    35161 tgagctgacc gacagcgcca cccgcggcgt cggcgccatg gaagaacagg tccgtcagat
    35221 cgccgaagcc ctcgccagca cggccgacga agccccgcca gccgcaccgg ccccgcgccg
    35281 cacccgcaaa ccgcgcgccc agtaagcacc ctgcgcacga tgggcggggg ccgccacacc
    35341 cgacgcgacc tttggcgcta gaatccggcc cgggacgggc ggtgcccgtc cccgtctttc
    35401 cagccaggat ccccatgaac gcccgctccg ccctgtacca ggcccgctac ggcaacccgg
    35461 acgtcgccgt tccggccgca gcctcccctg tcatcgacca cctgctggcc cacgcctcgg
    35521 tccgcgccta cctgccggac ccggtcagcg acgacacgct ggcggccatc gtggccgccg
    35581 cccagtccgc tgccagctcg tccaacctgc atgcctggag cgtggtcgca gtacgcgatg
    35641 ccgcccgcaa ggtccgtctg gccgactacg ccggcgacca ggcccacatc cgccaggccc
    35701 cgctgctgct cgtctggctg gccgacctgg cccgcctcga acaggtcgcc cggcgcgtcg
    35761 gagaaacctc cgccgcactg gactacctgg aaatgttcga ggtggccgtc atcgacgccg
    35821 cccttgccgc ccagaacgcc gtggccgccg ccgaatccct cggcctgggc acggtataca
    35881 tcggcgccat gcgcaaccgc ccggaagacg tggccgcgga acttcagctg ccaccgcggg
    35941 tggtggccgt cttcggcctg tgcgtcggca tgccggaccc ggcggcgccc gccgcagtca
    36001 agccgcgccc gccgcagtcc gtggtcctgc atcaggaaac ctattccctg ccggcccagg
    36061 aagccggcct cgagacctac gaccaggcca tggcggcctt ctacgccgcc caggggatga
    36121 aggtgcgcgg cacctggtcg aaccattcgg ccaagcgcat ccgtgacgcc gcatccctca
    36181 ccggccgtga ccgtctggca gaggcgctgc gcaacctggg ctttccgctg cgctgaatag
    36241 cacgggacgc ggcggtcttt accgtccgcg ccccgctgcg cccgaccgct ggcaccaccc
    36301 ggcctacatc cacttgcgcg ccgggttacc gcgccaggtc tcgcctgccg gcaccttggt
    36361 gccgggcatg aggtaggaac cgcccatcaa cacggcgttc tcgccgatct cggaaccggg
    36421 cagcaccacc gaattgacgc cgatggtggc gcccgcgccg atgatcacct tgccgcaggt
    36481 gagcttgccg ccgtagagca cgttgcccga caccagcgcg ttgtgcccga tcaccgcccg
    36541 cggtccgatc tccacctggt aggggtcgtc gatcgtgccg ccgatggcca cgtccgcccc
    36601 cacccgggcg ccatacaggg cctccaccac cggtcgcgtg aacaccgtgg tgaaaggcag
    36661 cagcgcgccc tggcccaggc gatagacgat ggtgatgagc ttccagaacg taaacaccgg
    36721 cgaatcgccg tcgtactcgc ccggcgcgat gggctgcaca cgcagcagca cccgcaccgc
    36781 gacggctgac agcaggccgt agaaaagcag cagcagcagg aagtccgcca ccacgtgata
    36841 gtccgccagc gcggccgtcg tgagcgggcg cagcgcccac acgccggcca ccgcgaggag
    36901 caggatcagg gtgatggcga tcgtgctcag cacgtaatcc agcggactcg tcttcacggg
    36961 gcggcctccg ggctcaggaa cggtagtcgg cgttgatctt cacgtagtcg taggagaagt
    37021 cgcaggtcca cagggtggcc gccgcatcgc cacgggccag atccacccgc accgtgatct
    37081 ccgccgcctg catgatgcgg gcaccgtcct cttcccggta gctcgccgcg cggccgccct
    37141 gttcggccac cagcacctct tcaccctggc tgcccagcca cacccgcagg gccgccacat
    37201 ccaggtcggc gatgccggcg tagccgatgg ccgccaggat gcgccccagg ttggggtcgg
    37261 aggcgaagaa ggcagtcttc accagcgggg agtgaccgat ggcatagccc accttgcggc
    37321 attcctcgcg atcccgcccg ccgccgatct gcagggtcat gaacttggtc gcgccttcac
    37381 cgtcgcgcac gatggcctgg gccagacgga tggacacgtc cagcaccgct tcgcggaacg
    37441 cggcgtagtc ggccgaggct gtgtcggcaa tcgtgccgtt gccggcctgg ccggtggcga
    37501 tgaggatgta gctgtcgttg gtggaggtgt cgccatccac ggtgatgctg ttgaagctga
    37561 gatcggtcac ctccttgagc aacgcgtcca gcacgggctg ggccacgtcc gcgtcggtgg
    37621 cgaggaagcc cagcatggtg gccatgttgg gcttgatcat gccggcgccc ttggacatcc
    37681 ccgtcagggt gatggttttg ccacccacct gcacctggcg cgacacggcc ttggcgaggg
    37741 tgtcggtggt catgatggcg tgggccgcgt cgaaccagcc gtccgccctg agggcggcgc
    37801 gggccgccgg caaaccggcg acgaggcgct ccacgggcag cggttcgaga atcaccccgg
    37861 tggagaacgg cagcacctgg tccgcagcga ggccgagctc tgccgcaacg gcctcgcagg
    37921 tggcgcgcgc cgccgcgagc cccggctcgc ctgtgcccgc gttggcgatg ccggtgttga
    37981 tcaccagcgc gcggatggtg cccgccggga gatgggcctt gcacacctga accggtgcgg
    38041 cgcagaagcg gttggtggtg aacacgcccg ccacgcggct gccgggggcc agctcgatga
    38101 gggtgagatc ccgccggttg gccttgcgga tgccggcttc ggccacgccc aggcggacgc
    38161 cgggcacggg cagcaggcag acgggatcgg gggtcgagag caggacgggc atggcagtac
    38221 tccggaagcg gacggtcaca aagcttggga tgataatccg gagcgcgtga caggcgcatc
    38281 cctcgaccgg gattttcatg cggcgtgcgg acagtgccag gcaagcctgc cgcgccaacc
    38341 gccgatccgc ccggtctact gcccttgcag ggacaggccc acggccaggc tggcctgcaa
    38401 cacgaaatgc tggaaagcgt gccacccggc ttcatccagg gcccgcccgt ggccgtcggc
    38461 gtagaacacg cccacctgtc gactgccggt cccgaagggt gccaggtagg cgtgtcggct
    38521 gccggctacc ttgagcaggc gctcggcgcc cggctgggcc gcgcggctgg gcgtcagcca
    38581 gcagccctgc gcactgtcga tggccgcatc gagcaggtcg tcaggctcgc cgtccatgga
    38641 aaacacaaag cttgccgcca gggcctcggc gccggatcca atggcggcgc gtccctgcaa
    38701 ttcactgcgg tcggcactga gtcgcgccac cagtacccgt tcgcagccga ccccccggta
    38761 caggccttcc agcaccatct gcaggatgtc gtcgaggggg gcctgggcga gggtcatggc
    38821 cgccacgtcg tgcaggatgt gcagttgcag atggggatcg gccacaccga ctggcggcgc
    38881 tgccgggtcg gcctgagccg gcccgctgcc tgcagacgcc gcagcgcccg caggtggagc
    38941 aggcgccccg aaggaactgg cgatacgggc cgcctcggcg gcattggcgc gagccgcatc
    39001 gagcacggcg gcgaatggca gctccagata atcggcaagg gcctgcacgg cctgccgcgc
    39061 agggggcgca tcccacccct gctccgacgc gcgggcaagc cgatgaccga gaagcaccgc
    39121 ctgctcaccg ttccgaccgc gggactgacc ttccagcacc gcctgggcca gcgcgcccag
    39181 gtgccattcg cgcaccagac cggcggacag atgccgcagc ggaaagccca gcaccacctg
    39241 ctgggcatcc tcttcgcgca tccgcccctg ggccagcagg ccatccaggc tgcgcgcctg
    39301 atcaccgccg aaacaccaga aggccatctc gcccacgcgg gtcagcaggg ccgcgatgaa
    39361 gacctcctcg ccgtgccctt cgccccgttt ggccgccgcc cagcgtgcct gcacggcggc
    39421 gtgaaagctg cgcgccatct cggcttcggc gcgcccgcgc acgccgccgg agagcagtgc
    39481 gtcgatgagg gagacggaca gggcgatctg cgcgactgga ttgaacccga ggacgacgat
    39541 ggcccgcgac acggtgctga cggtctggcg ggaggtgttg aagaaggcgc tgttggcgag
    39601 gcgcagcacc ttggccgtca tcggcgcatc cttgaggatg acctgggcga gggtcgaggc
    39661 cgagacccga tcatcgctga gcagggtgcg cagcgattcg acggtacgac cgaagacagg
    39721 catgtcacga ccgcggatgc gttcgatcca gccggtcagg tcgagaggat gggcgggaag
    39781 ggtcatggcg gggcctcacc ccttcaacgg cagactgccg cacgggcttg aggcgccccc
    39841 gccaaaaagt cagggccggc acttgccggc cccggatgtc tgcacctacg cgtagcgtca
    39901 gttcaacttg ccgtgacagt gcttgtactt cttgcccgac ccgcaggggc agggatcgtt
    39961 gcgaccgacc ttgggcgtca gattgacagc aggctgcgcg acgccggcgt ccggattggc
    40021 ggtggccagg gcctcgtcgt agtcggcgtg gtggtactga acgttttcca gctcgggcgg
    40081 cgcctcggcc tcgtcgagct gggcctcgga gcggatctgc acggtcatga gcagcttggt
    40141 gacctccacg cggacgacgc tgaggaggtt ctcgaagagc tcgaaggcct cgcgcttgta
    40201 ttcctgcttg ggattcttct gggcgtagcc gcgcaggtgg atgccctggc gcaggtggtc
    40261 cagcgccgag aggtgttcgc gccagtgggt gtcgaggctc tgcagcatca cgttgcgctc
    40321 gaagccatgc caggccgccg catcgaccag gctgatcttg tccgcgtagg ccgcatttcc
    40381 ggcctccagg atgcgctgca gcaggtcatc gtcggtcagg tcgggttcgg ccttgagcca
    40441 gtcgcccacc ggcagcttga gctggttttc ggactgcagc gccagttcga gggcggggat
    40501 gtcccattgc tcctcgacgg actcggcggg cacgtaggtg cgcagcagat cgtgcagcac
    40561 gccttcgcgc atggcggtga tggtctcggt gatgtcggtg gattcgagca gctcgttgcg
    40621 ctgctggtag atgaccttgc gctggtcatt ggccacgtcg tcgtattcga ggagttgctt
    40681 gcggatgtcg aagttgcgct gttcgacctt gcgctgggcg gtttccagcg aacgggtcac
    40741 catgccggcc tcgatcgcct cgccttcggg catcttgagg cgaaccatga tggcgttgag
    40801 gcgctcgccg gcgaagatct tcatcagcgg atcttcgagg gacaggtaga agcggctctc
    40861 gcccgggtcg ccctggcggc cggagcgacc ccgcagctgg ttgtcgatgc gccgcgactc
    40921 gtggcgttcg gtgccgatga tcttgaggcc gccggcctgc agcacctgct cgtggcggac
    40981 cttccattcc tcgcgcagcc gggagatctg cgcctccttg tcggcatccg gaatgttctc
    41041 gtcctcgcgc agcagggcga tgggcttgtc cacgttaccg cccagcacga tgtcggtacc
    41101 ccgccccgcc atgttggtgg cgatggtgat caccccggga cggcccgcct cggcgacgat
    41161 ctcggcctcg cgggcgtgct gcttggcgtt gagcacgtta tggggcagct tggcgcgggt
    41221 cagcaggtcg gacaggaact cggaattctc gatggaggtg gtgcccacca gcacgggctg
    41281 gccgcgctgc acgcagtcct ggatgtccgc caggatcgcg tcgtgcttct ccttcatcgt
    41341 gcgatacacc ttgtcgttct ggtccttgcg gaccatggga cgggcggtgg gcacgacgac
    41401 ggtctcgaga ccgtagatct ggtggaattc gtaggcttcg gtgtcggccg tgccggtcat
    41461 gccggacagc ttgccgtaca tgcggaagta gttctggaag gtgatggagg ccagcgtctg
    41521 gttctcggcc tggatgcgcg cgccttcctt ggcctcgacg gcctggtgca ggccctccga
    41581 ccagcgccgg ccgggcatca ggcggccggt gaattcgtcc acgatcacca cttcgccgtt
    41641 ctgcaccacg taatgctggt ccttgtggta cagggcgtgg gcgcgcagcg cggcgtaaag
    41701 gtgatggatc agcaggatgt gggccggatc gtagaggctg gccccttccg gcagcaggcc
    41761 caggcgaccg agaatctcct cggcgtgctc gtggccctgc tcggtgagca gcacggagtg
    41821 ggccttgagg tccacggtgt agtcgccggg ctgggtgatg tcctcgccct cgccttcctg
    41881 acgggtcagg agcggcggca ccgcgttcat gcgaatgtaa agctcggtgt gatcctcggc
    41941 ctggccggag atgatcagcg gcgtgcgcgc ctcgtcgatg aggatggagt ccacctcgtc
    42001 cacgatggcg tagtggaggc cgcgctggac gcgctcctcc aggctgtaca ccatgttgtc
    42061 acgcaggtag tcgaagccga attcgttgtt ggtgccgtag gtgatgtccg cctcgtacgc
    42121 cgccttcttc aattcgtggg gcatctggga caggttgcag cccacggaca tgcccaggaa
    42181 gcggtaaatg cgccccatcc agtcggagtc gcggctcgcc agatagtcgt tgacggtgat
    42241 cacatgcacg cccttgccgg ccagggcgtt gaggtagacc ggcagcgtgg cggtcagcgt
    42301 cttgccttca cccgtgcgca tttcggcgat cttgccgtca tgcaggacca tgccgccgat
    42361 gagctgaaca tcgaaatggc gcatgccatg cacccgcttg ccggcctccc ggaccacggc
    42421 aaaggcttcc ggcagcaacg catcgagggc ttcgccattg gccagacgct ggcggaactc
    42481 ggccgtcttg ccgcgcagcg cctcgtccga gagcgcctgg accgtgggct ccagcgcgtt
    42541 gatgacacgc accttctggg catactggcg aatcagccgg tcgttgcggc taccgaaaat
    42601 cttcttgaga aggcctgcga tcatttgatt tggaaggaaa aacgcggcaa aacggagatt
    42661 ctatcatggc accgggacgg gaccgtgacg cctgggggcg gcagcgcggg cttgtcgccg
    42721 cgcagcgtcc cgcctaggat gggggcgctt ctgccgattc caagccccag accatggccc
    42781 gcgccctcga cgcctacctc gaccgcggcg acaccctcgc ccgcctccac gcccacgccg
    42841 cgcgcctgct gcgcctgcag cgcaccctca gcgatgtgct gccggactat ctggccgcga
    42901 gttgccaggt cgccaacctg aaggacgagg tgctggtgat ccacacccgc ggcgccgccg
    42961 tcgcggtcaa gctgcgccag gcgatccccg gcctgctggc agactttgcc cgcgccggcg
    43021 tagccctgcg cgacatcaag gtgaaggtgg cggccccgga atcgccccgc ccggcggccg
    43081 caccggtgca tcgttccgtg agcgatggga cccgcgcagg gctggatgag ctggccgggc
    43141 gcctgccggc cgattcgccg ctggcccagg cgctgcgccg cttcgtccgc tcggcgggat
    43201 gagcctcagc caaaggccgg cgcgaggacc cgctccaggg ccaacagggc gaggaacacc
    43261 agcgcaacga tcagcgcata gcgcgccagc cgcagggcga acgcgaggta gcggcggtcg
    43321 cgggtgaaca ggtaggcaaa ggccgaggcg ccgatgccga tggccaccag caccgcgatc
    43381 agtcgcagaa gcagcacggc cgcggcgccg atcaggcgag cgccagatcg aacagatggg
    43441 cgaccgccgg cggcgtgcgg gcgtcttcct cgaaggtgac gatctcccac gcgctgcggt
    43501 cttcgagcag cacccgcagg atctggttgt tgagggcgtg gcccgacttg taggcgctgt
    43561 aggcagccag cagcgggtgc ccgaccaggt agagatcgcc gatggcgtcg agcaccttgt
    43621 gcttgacgaa ttcatccgcg tagcgcaggc catcggcatt gagcacgcgg tattcatcca
    43681 tcacgatggc gttgtccagg ctgccgccca gacccaggcc gttggcacgc atgaactcca
    43741 cgtcctgcat gaagccgaag gtgcgcgccc gcgccacctc acgggtgtag gcgtgctcgg
    43801 caaaatccac ggtgacagag gtgccggtac ggtcgatggc cgggtggttg aagaggatgg
    43861 agaagctcag cctgtagcca tcgtagggct ccagcctgac ccacttgtcg ccctcacgat
    43921 actccacggg cttgatcacc cgcataaagc gcttgggggt gttctgttcc tcgatgccgg
    43981 ccgactggat caggaacacg aagggcccgg cgctgccgtc gagaatggga atctcggcgg
    44041 cgtccacgtc gatatggacg ttatccaccc caaggccggc cagcgccgac atgaggtgct
    44101 cgacggtatt gaccttggcc gttcccttct gcagacccga gcacatgcgc gtatcgcaca
    44161 cggcgtacgg atcggccggc agatccacgg gcggatcgag atcgacccga tggaagacca
    44221 cgccggtatc cggcggagcg ggccgcagac tgagcgtcac cttgttgccg ctgtgaaggc
    44281 cgacaccggt ggtgctgatc gcggatttca gggtgcgctg cttgagcatg atcgcgcctc
    44341 ggatgcagag gtctgaattt taacacgccc accgtgctgc actgcgcaat gccacgccga
    44401 ccctccaaag acaaacagcg catgaacgga cgggaggcgg cgtccgcgca tgcgctgctg
    44461 tcgtccggcc atcagaccgg cgggtgctca gtcggcctgc ttgcgcagga acgccgggat
    44521 gtccagggtg gacacgccgc tggcggacat cgcctcgacc gtggtgcggg acgtgcggcg
    44581 gatcaccgcc ggcagttcca gattgccgta atccacttcg acgccgatgt tgtcggtacc
    44641 ggtccgcagc acctgcatct ccggcttgcg ggccacagcc gcccgcacgg caccgagacc
    44701 ggtggcgacg accgtgacgc ggatgcggtc ttccatattc tcttcgaaca cggtgccgta
    44761 cttgacgaag gcttccggcg cggcgaaggc ctgcacggtc ttcacggcgt cccgcacttc
    44821 ggacatcttg agggaacggc tggcggtgat attcaccagc acgcagcggg cgccggagag
    44881 ctcggtgcct tccagcagcg gcgacacggc ggcctgctct gcggcgatgc gggcacggtc
    44941 gaggccggcg gcttcggcgg agcccatcat ggcacggccc atctcggcca tggcggtacg
    45001 cacatcctgg aagtccacat tcaccaggcc gggcacattg atgatctcgg cgatgccgcc
    45061 caccgcactg gtcagcacgt tatcggcgga gcggaagcag tcttcgaagc cggcgtcgtc
    45121 gccatagact tcgaggagct tgtcgttgag cacgacgatc agcgagtcca cctggcggga
    45181 gagctcttcc acgccgcttt cggccacccg cagacggttc tcgaaatcga agggcttggt
    45241 caccacggcg accgtcagca cgcccatttc cttggcgacc tcggccacca cgggcgctgc
    45301 gccggtgccg gtgccgccgc ccatgccggc ggtgatgaag cacatgtggg cgccttcgag
    45361 cgcagcggcg atctgctcgc gggattcctg ggccgcggcg cgaccggctt cgggcttgga
    45421 tccggcaccg agaccggtct tgccgagctg aaccttgacc ggcgcaagac agcgggtcag
    45481 cgcctgggca tccgtattgg cgcagatgaa atccacgccc tgcacgccct gacggatcat
    45541 gtggtcgacc gcgttgccgc ccgccccgcc gatgccgatc accttgatgc tcgtgccacc
    45601 cgacgccttc tcaacgattt caaacattta catagcctcc aagagaatct gtactgcctg
    45661 cgcgctgcgc accggttgac ctatatatag tattaacgga agagtttaca actaaataag
    45721 ggtaccggcg caactttatt cgtccggatg gatgtctccg gcgaccctca aaaattccgc
    45781 tcgaaccagg accgcatccg cgagaacacc tgccccacat tgcgcgtctg gcgagcctgg
    45841 atgccccgcc ttttctgggc ggcgccttcc agcaccaagc ccatcgcgtt tgcataacgc
    45901 ggctcgcaga cgacctccgc cagcgcaccg ctgtagttgg ggtatccgat gcgcacgggc
    45961 atgtggaaca cctcctcacc aagctcgacc atgccctgca tgaccgccga accgccggtc
    46021 agaacgatgc ccgacgagag cagttcctcg tacccgctgc gccgcagttc ggcctgcacc
    46081 agttcgtaga tctccgacac acgcggctcg atcacgtcgg ccagggcctg acgcgagagc
    46141 ttgcgcggcg gacggtcgcc gacgccgggc acttcgatca tctcggccgg gtcggtcagg
    46201 gtgtgcatgg ccacgccatg acggatcttg atctcctcgg cttcggcggt cggcgtacgc
    46261 agggccatgg cgatatcgtt ggtgatctgg tcgccggcgt agggaatcac cgcggtgtgg
    46321 cggatagcgc cctgggtgaa caccgccagg tcggtagtcc cgccgccgat atccaccagg
    46381 cacacgccca gttccttctc gtcctccgac agggtggcgt agctggacgc cagcggctgc
    46441 agcaccagat cgagcacttc caggccacag cggcgcacgc acttgacgat gttctgagcg
    46501 gccgacaccg cgccggtgac gatgtgcacc ttcacttcca ggcgcacgcc gctcatgccg
    46561 aggggctcgc gcacaccgtc ctggccgtcg atgatgaatt cctgggtcag gatgtgaaga
    46621 atctgctgat cggccggcag aggcatcgcg cgggcggttt cgatgacccg ttccacgtcc
    46681 atgggcgaca cttccttatc cttgatcgcc accatgccgt tggagttgaa gctgcggatg
    46741 tgactgccgg cgatgcccgt gtagacatcg cggatcttgc agtcggccat cagctccact
    46801 tcctggatca cgcgggagat ggcatcgacg gtggattcga tattcaccac cacgcccttc
    46861 ttgagccccg acgagggctg ggtgccgagc ccgatcacgt ccaggcggcc gtcgggccgg
    46921 agttcggcga ccacgcaggt gatcttggag gtgccgatgt cgaggccgac gatcagttcc
    46981 ttgtactctt tgctcatcac ttccctttgc tgtcttgctt gccggccggc gcggccacgg
    47041 gcaccgcgcg cagggcaaaa ccattggggt agcggaggtc caccaccgcc acgttcatgt
    47101 tcaactgggc cttggcccgg gggtaataac gcacgaagcg gtcgatccgc tcgtccagcg
    47161 gggccttggc ctggtcgtgg cccagttcga tggtgagtcc atccgccatc ttgagctgcc
    47221 acgcctcacg ggctgacagg gagacaccca ccagtccgcg gcccagggga ccgagcttgt
    47281 ggctgaactc gtcaaacttg gccaggagga cgcccgacga gccctccggc ccgctgaaga
    47341 tcggcatcgg cgcgttgctc gcggcagaga agatctcgcc gtagcggttc accaggcgca
    47401 tgtcgcccgt gtcgccaaca cgccagtagg cggaggccac gtgctcctcg aggcgcacct
    47461 cgacgccgcc cggccagcgc cggcgcacgt ctgcgcgtcg cacccagggc agcttttcga
    47521 aggcgttgcg cacctcctcc agattgaccg tgaagaagtt gcccttgagg gacgaacgcg
    47581 ccgcgtattc gagttgttcg gtggtcacct gggcgagggg cgtggtgacg accacttcct
    47641 tcagcgggaa ggccggcagg cgcgacaggc ccgccacggc cgcatagccg aggccggccg
    47701 caccggccac cagcagcaga tcggcgatca ggttgaggag ctgggggcga tcccacaggc
    47761 cgttacctcc tccgtcgcgc ttctgcgcgc gcgccgcagg ccgcggccgc cggggcgacg
    47821 acggcttggc gccggcttca ggcggcacgt tggaggtggc gcggcgatca accaaggcgg
    47881 gctcccgcca ggatctcgag acacagggcc tcgaaggaca gtccggacac gcgcgccgac
    47941 atggggacca gagaatggtt ggtcatgccc ggcgcggtgt tgatctccag cagcgtgtag
    48001 cggccgtcgt cctgcaggat caggtcggca cggccccagc cgcggcagcc cagcaccttg
    48061 gcgcaacgca cgatggtggc ctggatgtcc ttttccaggg cctccggcag gccgctcggg
    48121 cagaaatact gggtgtcgtc ggtgaagtac ttgtgctgat agtcgtaatt gccatccggc
    48181 gcgacgatgc gcaccagcgg cagcgcccga tcccccagga agggggcggt gagttcctgg
    48241 ccggacacga attcctcggc cagcaccaga gagtcgtact gcgcggcaag ctcccaggcg
    48301 cccttgagct gggccgcctc ggtgaccttg gtggcgccca tgctggagcc ctcatgcacc
    48361 ggcttgacga agatcggcag gcccagctgc gccaccacgg catcccagtc gctgtcggcg
    48421 gtcaggatcg cgaagcgcgg agtgggcagg ccgctggcca gccacacgag cttggtgcgc
    48481 cacttgtcca tggcgatggc cgatgccatc acgccgctgc cagtataggg aatgcccatc
    48541 agttccaggg ccccctgcac cgtgccgtcc tcgccgccgc ggccgtgcag ggcgatgaag
    48601 gcgcgctcgt agccttcctc cttgaggatg tgcaggtcgg tctcggcggg atcgaaggcg
    48661 tgggcgtcca cgcctgcccc ttgcagggcc gccagcaccg ccttgcccga catcaaggag
    48721 atctcccgct ccgccgacga gccgcccagc agcacggcga cctttccgaa ttgactgcgc
    48781 actgcgtcca ccctcagacc tctccattca ccagtttgcc cggcacagcg ccgatgctgc
    48841 cggcgcccat ggtgatcacc acgtcgccgt cctgcgccgc ggccaggatg gcctgcggca
    48901 tgtcctcgat gtgttccacg aacaccggtt cgatgcgccc cgccacacgc agggcgcggg
    48961 ccagggagcg tccgtcggcc gccacgatgg gcgcctcgcc ggcggcatac acctcgccca
    49021 gcagcagcgc atcggcggtg gacagcacct tcacgaaatc ctcgaaacag tcccgcgtcc
    49081 gcgtgtagcg gtggggctgg aaggccagca ccagacgcct cccgggaaat gcgccgcgcg
    49141 ccgcggcgag ggtggcggcc atctcgaccg ggtggtggcc gtagtcatca atgaccgtgg
    49201 ccgccccgcc cgccggcagc gccacctcgc cataacgctg gaagcgccgg ccgacgccct
    49261 tgaagtccgc cagcgccttg acgatggcct cgtcggagac gcccacctcg gtggccacgg
    49321 cgatggcggc cagggcgttg agcacgttgt gacggcccgg caggttgagc accacggaga
    49381 tgcggctggt ggtgccattt acccgcacca cgtcgaagcg catgcggccg tcttcggcgt
    49441 ggatgttctc ggcgcgcacg ttggcggatt cggagaagcc gtagcgcacg atctgcttgg
    49501 acacgagcgg catgatggag cgcacgtgct tgtcgtcgtc gcacagcacc gccacgccgt
    49561 agaaaggcag gtgctgcagg aaatccacga aggactgctt gagccgggcg aagtcgtgcc
    49621 cgtaggtttc catgtgatcg gcgtcgatgt tggtgacgac cgagatcacc ggggtgagaa
    49681 gcaggaagga ggcgtcggat tcgtccgcct cggccaccag gaaatccccc ttgcccaggc
    49741 gcgcattggc gccggcggcg ttgagcttgc cgccgatgac gaaggtcgga tccagcccgc
    49801 cttcggccag gatgctcgcc accagcgagg tggtggtggt cttgccgtgg gtgccggcaa
    49861 tggcgatgcc ttgcttgagg cgcatcagtt cggccagcat ctgggcgcgg ggcacgacgg
    49921 ggatctggcg cgcgcgggcg gcgatgactt ccgggttgtc gcccttgacg gcggtggagg
    49981 tcaccacagc gtccgcgtcc tgcacgttct cggcggcgtg gccctgcatc acgcgggcac
    50041 cgagttcctt gagacggcgc gtcgcagcgt tgtcgcccag gtccgagccg ctcacctgat
    50101 agcccaggtt gaccagcact tcggcgatgc cgctcatgcc ggcaccgcca atgccgacga
    50161 agtggatgct cttgactttg tgtttcacga ttcttccttt acgcagcgag gccggcgcat
    50221 acgtcggcga cgcgggaggt ggcgtccggg cgggcccgcg cccgcgcctt ctccgccatt
    50281 tccagcaggc gctcgcgatt caggctgccc agcagcgcgg ccaggcgcgc cgggtcaagt
    50341 tcggtctggg gcagcagcac cgccgccccg gcatcggcga ggaaacgggc attgccggtc
    50401 tgatgatcgt ccaccgcgtg aggaaagggc accagcacgc tggccacgcc ggccgcggcc
    50461 agctccgcca cggtgagggc gccggcgcgg cagatcacca ggtcggctgc agcgtagcgc
    50521 tcggccatgt cgtcgatgaa gggcagcagc tcgccctcga caccagccgc ccggtaggcg
    50581 gcgcgcaggg cgtcgatctg gcgcgtgccg gcctgatggg tcacctgcgg acgctcggcc
    50641 ggcgggattt gcgccagggc cttgggcacc gcgtcgttga gggccgcggc gccgaggctg
    50701 ccgcccacca ccagcacccg cagcgggccg ctgcgaccgg caaagcgctg ggccggtggc
    50761 gccactgcgg cgatgtcggc gcgaaccggg ttgcccgccc actcgccctt gcgcagcacg
    50821 tccgggaaac cggtcatgac cttgtcggcg acgcccgcca gcacccggtt ggccagcccg
    50881 gcgaccgaat tctgttcgtg caccaccacc ggccggccga tgagcgcggc catcatgccg
    50941 ccggggaagg tgacgtatcc acccatgccc agcaccacgt cgggccgcac acggcggagc
    51001 tgggccaacg cctgggcgaa gccacgcagc aggttgaaag gcagcagcag cttgcgcagc
    51061 aggcccttgc cgcgcagcgc ggtgaagcgc acccacgcca tgtcgtagcc actggccggc
    51121 acgatgcgcg cctccatgcc gtccgggttg cccatccaga cgaccttcca gccccgcgca
    51181 cgcagggcgt cggccaccgc caggccgggg tagatgtgtc cgcccgtgcc gccggccatg
    51241 accatcaggg tgggcatcac gcgttgcctc cgcgggtgat ctggcggttt tcccagtcga
    51301 tccgcagcag gatcgcgagc gccacgcagt tggccaggat gccagaaccg ccgaagctca
    51361 tgaggggcag ggtcaggccc ttggtgggca gcaagcccat attgacgccc atgttgatga
    51421 aggactgcac gccgatccag atgccgatgc cctgggccac cagggccgca taggtgcgct
    51481 ccagcaccag ggcctgcttg ccgattgcga aggcccggtg gatcaggatt ccgaacagga
    51541 ccaccacggt gaacaccccg gcgaaaccca gttcctcggc gatcaccgcc agcaggaagt
    51601 cggtatgggc ttcgggcagg tagaagagct tctcgatgct gcctcccagc cccacgccaa
    51661 accactcccc gcgcccgaag gcgatcagcg cgtgggacag ctgatagccc ttgccgtagg
    51721 cgtcctgcca gggatccatg aagcccagga tgcgctgccg gcggtattcc gagaaggtga
    51781 tcagcaacac gaagcccact gcggccacca ctgccagcaa cacgaacagg cgcacattga
    51841 tgccgcccag gaagaggatg ccgaaggcaa tggcggtgat gaccacaaag gcgccgaagt
    51901 ccggctcctt gaggagcagg aagccgacga agaggatgac cccggccatc ggcgcaaagc
    51961 ccttcttgaa gctgcccatg tagggcagct tgcgcacggt gtaatcggcc gcgtaaagcg
    52021 cggcgaagag cttcatcaac tcggacggct gcagattgac cacccccagg ggcagccagc
    52081 ggcgggcgcc gttcacatcg cgccccacgt ggggaatcag caccaccagc agtagcacga
    52141 caccggcgat gaagagccac ggcgcgatct cctgccaggt acgcatgggc acctggaaga
    52201 ccaccacgcc ggcgatcacc ccgatggcaa ggaaaaccgt gtggcggatc aggaagtagg
    52261 ccggctggta gctgaagctg cggctgccct cggccgtggc gatggacgcc gagaacacca
    52321 tgatcagccc cagcagcagc agcgccgccg cggaccagat cagcagcgga tcgagctccc
    52381 gcggctggcc gagggggcgc atcaggcggc gcacggcgcg gctggcctcg gactgggacg
    52441 cggcgccggc gtggcgcgag aagaagccgg aaaggctggc ggtcaggctc atgcggggcg
    52501 cactccgggc aggcgccgca ccgcgtcgat gaagacctgg gcgcgatggg cgtagttgcg
    52561 gaacatgtcg agactggcac aggcgggcga caggagcacc acgtcgccgg cctgggcccg
    52621 ggcgtcggcg gcgagcaccg cggcgtccat gtcggcggcc gggatcaggg gcacgccgca
    52681 accggccacg gcggcttcaa tggccggcgc gtcacggccg atcagcacca cggcgcgggc
    52741 ataacgggcg aaggcgtcct tgagcggcga aaagtcctgg cccttgccgt caccgccgac
    52801 aatcagcacc acgcgggcac gcaggccttc cagcgcggcc acggtggcgc ccacgttggt
    52861 gcccttggag tcgtcgtaat acaccacgcc gtcggcccgc cgggcgacct tctcgacccg
    52921 gtggggcagg ccgcggaagg ccttcagcgc ggccagcagc ggcgcccgcg gcaggccggc
    52981 ggcctcgcac agggccagag ccgccagcgc gttggcgatg ttgtggttgc ccggcagggg
    53041 caggtcggcg cgcgccagca gacgctcgtc gccgcgcacc agccactcgc cgccggcgtc
    53101 ggtacgcaga ccgaaatcgt ccggcccggc cggcgcgtcg acgccgaagc tgtacacccg
    53161 tcgccccggc acggccatgg cggcaacccg ggcgtcctcc cggttcagca cctgcacgcc
    53221 ggcgccctgg aacaccgcgg ccttggtggc cgcgtagtcg tcgagcccgg cgtaacggtc
    53281 gaggtggtca tcggtgacgt tgagcacggt ggccgatatc gcctcgagtg ccggatgcgt
    53341 ctccagctgg aagctggaca gctccagcac ccacacggcg ggcagggcct cgccggcgtc
    53401 caggcgcgcc atcagcacgt ccagtgcggc cgggctgatg ttgccggcca ccacgccatc
    53461 cacgccgcag gcgcggacca tctcgcccgc aagagtggtg gtggtggtct tgccgttggt
    53521 gccggtgatg gcgatgagcc gcgtcgcgtc gcgcacaccc agatcgcgca gggcctggac
    53581 gaagaggtcc atctcgccga cgacggtgag gccgcgggcg cgggccgtcg ccacgagggg
    53641 ctgccgggga tcgaggccgg ggctgatggc gatgcgcgtc acgccttcga ggagcgcgtc
    53701 actgaagggg cccgcgacca cctcggcggc gggcgcctca gcccgcaggc gctcgacgcc
    53761 gggcggcctc tcgcgggaat ctgccacccg cagcgccaca ccctggcggg ccagccagcg
    53821 ggccatggcc agtccggatt cgccgagacc gaggatgagg gtcagatcag ccgccatgtc
    53881 cggtcaccgc agcttgaggg tggacagccc gaacagcacg agcatgatgg tgatgatcca
    53941 gaagcgcacc accacctggg tctccttcca gccgcccagc tcgtagtggt ggtgcagcgg
    54001 cgccatgcgg aagatgcgct tgccgccggt gtacttgaag tacatcacct gcaccatcac
    54061 cgacagggcc tcggccacga agaggccgcc catgatgaag agcacgatct cctggcgcac
    54121 gatcaccgcg atggtgccca ttgccgcgcc cagggccagg gcgcccacgt cgcccatgaa
    54181 gacttccgcc gggtaggcgt tgaaccacag gaagcccagt cccgcaccgg cgatggcgcc
    54241 gcagatcacg gcgagttccc ccgcaccggg gatgtagggc atgcccagat atttggagaa
    54301 gcccatgtgg ccggccacgt aggcgaagat cgccagcgcc gcgctgacca tcaccgtggg
    54361 catgatcgcc aggccgtcga gcccgtccgt gagattgacc gagtggctgg tgccgttgat
    54421 gacgaagtag ctcagcacga tgaagccgac gacccccagc gggtaggtca ccgccttgaa
    54481 gaagggcacg atcagttcga tctgcgccgg cagcgtggcg gtcagaccca ggaaggtggc
    54541 cgcacccagc gcgaccatcg acgtccagaa gtacttccag cggctcgcca gtcccttcgg
    54601 atcgcgatac accaccttgc gccagtcatc cacccagccc accgcgccaa aacccagggt
    54661 caccagcagc accacccaca cgtacttgtt gcgcaggtcg ccccacagca aggtggtgac
    54721 ggcgatggag atgaggatca gcgccccgcc catggtcggt gtgccggcct tggtgaggtg
    54781 ggacttgggg ccgtcatcgc gcaccgcctg gccgatcttc ttggcagcga gccagcggat
    54841 caccggcgga ccgaagacca gcgacagcaa cagcgccgta ccggccgcca gcacggtgcg
    54901 cagcgtgatg tatccgaaca cgttgaagaa ccgggcgtcc ttgctcagcc attgggccag
    54961 ttcaagcagc atcgcgcacc tccggaccgg tcatgcacgc atccacccac gaatattccc
    55021 cgcaaaccct catcaagcct tctccgcgat ggcgtccacg acccgctcca tcttcatgaa
    55081 gcgcgagccc ttgacgagca ccaccgtatt gctgttcatc tcggccttca cggcggcgac
    55141 gagctcgtcg acccgctgga aatgccggcc gccgtccccg aagttgcgcg cggccacctc
    55201 gctcatctca cccagtgcaa agaggaaatc gacgccttcg ctcttggcgt agccgccgat
    55261 ctcgtcgtgg tactggaaac tctgatcgcc aatctcgccc atgtccccca ccaccaggat
    55321 cttgcgaccc ggcgtggcag ccagcacggc gatgccggcg cgcatggaat cgggattggc
    55381 gttgtagctg tcgtcgatca ccgtcgcgcc gccgacgccg gcccgcacgt gcagccgacc
    55441 cggtgtgcca ctgaacgccc ccaacccggc cgccacatcc tcgggccgcg cgccggccgc
    55501 cagacaggcg gctgccgcgg ccagggcatt gcgcacgttg tgttcaccgg gcacctgcag
    55561 ccgaaaggac gcggcgccct tcggcgacgc gagggtcagt tcactggcaa atccatggga
    55621 cgtgacctgg gccgtcacgt ccgcgcccgg ccgcagacca aaggtcagca cccgacggcc
    55681 cgcgttgagg gccttccagt agccggcgaa ggcatcgtcc gcgttcacca cggccacccc
    55741 gtcagcagac aagccttcgt agatcgcgcc cttttcccgc gccacttcat tgacgctgcc
    55801 catgccttcc aggtgggcac gctgggcgtt gttgacgagc gccacggtcg gtgcggccag
    55861 acgcgtgaga tagccgatct cgccgggatg gttcatgccc atctcgatca ccgcggcacg
    55921 gtggttgccg gacaggcgca gcagcatcag gggcaggccg atgtcgttgt tcaggttgcc
    55981 gcgggtggcg agcaccgcca gatccggatc aagcccctcg cgccgggccc gcgcacgcag
    56041 gatcgccgca cacatctcct tgacggtggt cttgccgttg ctgccggtca cgccgatcag
    56101 gggcagctcg aagcgcgcgc gccagccggc ggcgagctgg ccgaggccaa tgcgggtgtc
    56161 gtccaccacc agcagcggca atggcaccgg attggcggcc gcccaggcac gatccaccat
    56221 cgcggcggcc gcaccctggc tggccgcctg ggcgacgaag ccgtggccgt cgaagcgctc
    56281 gccgcgcagg gcgacgaaca gctggccgct ctgcagcgcg cgggagtcgg tgctcacgga
    56341 ctcgaacacg acgttcccgc ccaccgccgt accgcgggtc aggaccgcgg cgccctgcag
    56401 atccatcatg agagacctcc ctgggcatcg ttccaggctt ccagcgccgc gcgggtctgt
    56461 tccgcgtcgg aaaagggcag gcgcacgccg ttgatctcct gataaggttc gtgccccttg
    56521 ccagccacca cgaccacgtc gtcggcagcg gcacgcatca ccgcgtgacg gatggcctcg
    56581 gcgcgatcgg ggatgatgtc cacgtcgccc tgcacgccgg ccacgatggc ctgcaggatg
    56641 cgctgcgggt cttccgtgcg ggggttgtcg ctggtaagga tcaggcggtc ggccagacgc
    56701 cgcgccacct cgcccatcag cgggcgcttg ccggggtccc ggtcaccgcc gcaaccgaac
    56761 acgcaggtca gcgatccccc gcgcgcctct gccgtcggcc gcacggcccg caggacctgc
    56821 tccagggcat cgggggagtg ggcgtaatcc accaccacca gcggatcgcc gacgccgccg
    56881 agggtctgca tgcggccctg gggcggggtg agcccctcgg ccaccttgca cgcgtcctcg
    56941 aagggaatgc ctgaggccat cagcgtggcg atcaccgcca gcaggttgga ggcgttgaaa
    57001 cgccccacca cgttggcgaa caggtcggcc gagacattgc cgcaccgcgc ggtgaagcgc
    57061 aggcccaccg gcgtgatggt gagattttcg gccaccagca cacgctgggc gggtgcggcg
    57121 tcccggttgg atgggatcac ggtgtagccg atcacctcga caccgctgcc cgcgaggctc
    57181 cgcgcctgct ccatgccgaa gcggtcgtcc aggttgagga cggcgctgcg caagccaggc
    57241 atcgcgaaca ggcgggcctt ggcttcaccg taggcttcca tggtgccgtg gtaatcgaga
    57301 tggtcgcggg tcaggttggt gaacacggcc acgtcgatgc gcacgccgtt caaacgagcc
    57361 tgatcgagcc cgatggacga cacctccatg gccaccgcct ggccgccctg atcgcggatg
    57421 tccgcaaggg cgcgatgaac cgtcagggcg tccggcgtgg tgttcagcgt cgcgctgagc
    57481 tgtccgggaa aaccgatgcc gagggtgccg atcaatccgc accgacgccc cagctgggcg
    57541 taagcgcgag ccagccactg ggtgaccgag gtcttgccat tcgtgccggt gacgcccacc
    57601 gtccacaggg cctgggaggg ccggccgtag aattcatcgg cgatgtagcc ggacagatgg
    57661 gccagtccga agaccggtac attcggcacg tccacggcct cgttccactg atagccctcg
    57721 cgctcccaca gcaccgccga ggcaccggcg tgcaccgcat cgacgatgta gcggcgaccg
    57781 tcggcgcggt ggccgggata ggcgacaaaa atgtcgcccg ccagcacctg gcgcgagtcg
    57841 gcgcacaggc gggtcgcctc gacgccgtcg gcggcaagcc gggcgaggat gtcgctggcc
    57901 tggtgttcgg gactcacatg gactccttga cggccggcac gacgctctgg cgcgccacct
    57961 gcagcggctt gagcggcgcg tccggcgcga ccccgagggt gcgcagggcg ccgcccatca
    58021 cctgggcgaa caccggcgcg gccacgtcgc cgccgtaatg cttgccgttg gtcggctcgt
    58081 cgatcatcac cgccacgatc aggcgcgggt cggaaatcgg cgcgatgccc acgaaggagg
    58141 ccacgtactt ggtggtgtaa tgcccgcctt cgagcttcat ggcggtgccg gtcttgccgc
    58201 cggcgcgata accgggaatc gccgcctggg gcgcggtgcc gccctgctgc accaccagct
    58261 cgagcatctg gcgcacctcg cgggcggtgt gcggcgagaa gacctgcttg ccgtgcaccg
    58321 gcggcgcatc cacgcgggcg agggacaggg gcaccatgtc cccgtcgcgg gcaaacacct
    58381 ggtaggcgtt ggcgatctgc atcaggctca cggagatgcc gtggccgtag gacatggtgg
    58441 cctgctccac gggacgccag ctcttggccg ggcgcagacg cccgcccact tcacccggga
    58501 aacccaggtg cagtggcgcg ccgaagccca ggtcgtcgaa catcttccac atggtttccg
    58561 gcgggaaggc cagggccagc ttcgcagcac cgatgttgga cgacttctgg atcacctggg
    58621 cgaccgtcaa cgccccgtgg gggtgagcgt cagagatggt ggcggtgccg atggtcatcc
    58681 gccccggcgc gcagttgatg atcgtattgg ccgtcacctt gccggcctcc agggacatgg
    58741 cggccgagaa gggcttcatc gtggagcccg gctcgaacgt gtcggtgagg gcccggttgc
    58801 gcaactgggc gccggtgagc ttggtgcggt tgttcgggtt gtaggtgggc gcgttggcca
    58861 gggcgaggat ctcgccggag cgggaatcga tgacgatgat gccgcccgcc ttggccttgt
    58921 ggtcggtcat ggcctgcttg agggcggcgt gggccaggta ctggatcttg ttgtccacgg
    58981 ccagggcgac ctcacggccg tcctggggtt cgcggatcga ctggacgtcc tccacgacgt
    59041 tgccgcgccg gtccttgacc acccgccggg aaccgggctt gccggcaagc tgtggctcga
    59101 aggccagctc gatgccttcc tgacccttgt cgtccacgcc ggtgaagccg atcacgtggg
    59161 ccatcacctc gccgccggga tagaagcggc ggtattcgcg ctggtcgtgg acgcccggca
    59221 gcttgagggc cgcgatgcgg tcagccagct ccggtgagac ctggcgcttc agatagacga
    59281 aatccttgtc ggtgccgagc ttctggttca gatcctgtac cggcatctcc agcaggccgg
    59341 ccagctggcg gctctgggcg ggcgtcagct tggcgtcctc gggaatcgcc cagatggact
    59401 tgaccggcga cgagaccgcc agcggctcgc cctggcggtc ggtgatcttg ccacgggtcg
    59461 ccggcacgtc cagcacgcgc gcgtagcggg actcgccctt ggcctgcagg aagcgatcgt
    59521 tcacgccctg cagataggcc gcgcgcccga ccagggccag cgaaccgccg agcaggcaga
    59581 gcagcacgaa acgcggccgc cacgccggca gttcggagcg cagcagcgga ttgtggttga
    59641 aggtgacacc cttctgggtc ttgttcatgg ccgctcctcg atggcgacga tctgccccgg
    59701 ctgcggtgcg cgcatggaca gcttctcgcg cgcgagcttt tccacccggg catgggccgc
    59761 ccaggtgctt tgttccagtt gcaactgatc ccactccact tccagctggc gggtgcgctg
    59821 ctgctcccgc tcgaccgccg cgtagagctt gcgggcgcgg tgctgggacg ccaccacgcc
    59881 caggctgctg gcgacgatca gcatgatcag gaaacccgtg aggcgcgcca tctcaggcct
    59941 ccgtccgcgc ggccacgcgc atcaccgcgc tgcgggcacg gggattggcg gcgacctcgg
    60001 cgtccgacgg gcgcacctgc cgccccacca gggtgaggcg cggacgcggc aactccgccg
    60061 cccgcagggg caggcgtgcg ggaagctgcg gcggccgcga ttcgtcgcgc atgaagcgct
    60121 tgacgatgcg gtcctcgagc gagtggaagc tgatcaccac gagacggcct ccgggcacca
    60181 gtcgcgtcac tgcctggggc aggatcagcg acagttcctc aagctcccga ttgacgcaaa
    60241 tccgtagagc ctggaaggtg cgcgtcgcag ggtgctgacc cggctcgcgt gtccggaccg
    60301 ctttttccac gatcgcggca agctgtccag tggttgcaac atgcccccct gcccgagcag
    60361 ctgcaagcgc ctttgcaatc tcataagcaa accgttcttc accgtattcc ctcagcactc
    60421 gggttatctc tgcgaccggc gcctgcgcca gccactccgc cgcagtctcg ccgcggctcg
    60481 tatccatccg catgtccagg ggcgcatcga aacggaagct catgccgcgc tccgcctcgt
    60541 ccagctgcgg cgacgacacc cccagatcca gcaacacccc atccacctgc gccacgcgcc
    60601 gtgcatcgag ggcttcggcg aaatccgaaa acggcgcatg caccagctcg aagcgcggat
    60661 cgtcgaggcc ccgtccggcc gcgatggcca ccggatcccg gtcgaaggcg atcagccgcc
    60721 cctggtggcc caacttggcc agcaccgccc ggctgtggcc cccgcgcccg aaggtgccgt
    60781 ccacgtacag gccgtccggc ttcaccgcca gcgcctccac cgcttcctcg agcagcaccg
    60841 tcacgtgcgg cgcggacgtg gtcacagcgg caacgcgtcc cagttggcgg gcatgagctg
    60901 gtcgcccagg gcaaagatct gctcctgctg cttctgccag cccgcgtcgg accacagttc
    60961 aaagtgattg ccctgcccca ccagatgcac ctgcttgtcc agctgcgcaa aggcgcgcag
    61021 ctcgggcgcg atgagcacgc gaccggcgga atccagttct tcgtcgcggg caaagcccac
    61081 gaggagacgc ttgaggaggg cggaatgctg ttcgaggctc gacccggcga gcaccttgtc
    61141 gcggatcggc tcccaggcgg gcgccgggta gatcaggagg cagcggtgag gatgggcggt
    61201 gagcacgagc tgcccatcgc cctgcgcggc gagcgcatcc cgatgccgcg caggaatcgc
    61261 caggcgaccc ttcgcatcga gactgagcgc tgctgcgcct tggaacatct tctcccactc
    61321 ccccacaccg ccgccacggg aaaaaccctt ttctcccact ttggtgcact ttgccccact
    61381 ttagaggaaa aaaaataccc ggtcaagccg aaccgggccg ttttcccttt tggacacaag
    61441 cacttacgtg caaaaaccgg caaaacagga aatccgctta taaatcaacg gcaatagaaa
    61501 agattgcaaa agtggagcct taaaagctgg cgacacgccc ctgcgccagg ggctgtcatc
    61561 ggctttatgg cggcagtatc cggaagcccc tgacgacaca gggactttcg ccatttatag
    61621 gagtgaaccg ggtgaccggt ggagaaaagt ggggccgcgc cccggcgggc gggatgattg
    61681 ggggaaatgg tgggaagccc gccgataagc cgggttctgt cgagcgggtg gagcggactc
    61741 cacccaccgg gcagtcattc ctctggtcga cgcgttgccg cgccgctcta gcagcctacc
    61801 cggggactcc gcgagcagcg tcaacgtccc cctatttggc cttgccccgg atggggtttg
    61861 ccgtgccgtc cgtgtcacca cgtccgcggt gggctcttac cccaccgttt cacccttgcc
    61921 tgatcccctt gcggggccat cggcggtctg ctctctgttg cactttccgt cgctttgacc
    61981 cgaaggctat ggcgcccggc cgttagccgg catcctgctc tgcggggccc ggactttcct
    62041 cgatgcgcat gcgcaccgcg actgcctggc gaacttcccg gcgcggagta tacccgaagc
    62101 tccgcccgac accggtgccc gcctcaactg ccacgcagca ctgccatccg tgtcaccacc
    62161 gcatcgagga cacagcgcat ccgctcgcga cgtacgccag cctgacccca cgcggtgatc
    62221 tggccggcca cctcgatccc caggtcggtc tcgccgcagc cctctggtcc cgccagcccg
    62281 gccaggcgcc ccaccggccg gccgccgggg cgcacatccg caaagaccac caccacatcc
    62341 tgcgtgcggg gatcaacgcc cacactctga acgccggcgt tgcgcagggc gtcgccatac
    62401 tccgactcga cagcctgcgc cagggcgccg ccgccgtttg cggcacgggc tcccgacagc
    62461 tccggtccga gcacggccac cgcgccgacc cccaccacga tgagtacccc cagtcccatc
    62521 accagtctgc gtacgtccat gcttcactcc cgaagaaact gaccgccctg acggcacacc
    62581 tcgacggaag cttgaggaca tccgtcacgc cgcgccgcaa aggggcgccg tacctgccct
    62641 ccaccgccaa attgtaagta aagcttgcgc cgcgacgtcc cgcttgcatc ctgatacggc
    62701 cggcgggcgc ccgccggctt cgtggggatc acaatcaaag tatcgacaac aggaggtaca
    62761 ggtcatgaac ccccgtacga tccgaagggc tctgctcgcc gccgccgcgg gcattgccct
    62821 gatggccacc ggcgccgctt tcgccgaccg gggacatggc ggcggctggc acggcgcacc
    62881 gggtggctgg gcagcgcgcc cggcacccgg cggctactgg cactacggcg aacatcgcgg
    62941 cgcgtggggc tggtggtggg tgctgcccgg cgcctggtgg ctctatgatc ggccttacta
    63001 tccttacccg gccgcgccgc ctgtggtggt tcagccggtc ccgcaggagc ccgtcatcgt
    63061 ccagccggcc ccgcagccgg ccgcccagac gtggtatttc tgcgaaagcg cccgcgccta
    63121 ttacccctac gtccagacct gtgcggaagg ctggcgcccg gtcccggcag cgccgccgcc
    63181 ggcttcaggt gcgcagcaat ccgcaccggc cgccggctca ggaggtgtgc aatgaacgtc
    63241 cgtctcgccg tattgccgct gctgctcctg gccggctgcg ccaccctgcc cgacggccct
    63301 cgtgtcgccg tgatgcccgc gccgggcaag ccctttgacc tgttcgtgca ggaagagcgg
    63361 gcctgccgcg ccttcgccag ccagtccctg ggcactacgc ccaacgatct ggcggcccgc
    63421 aacgtggccg gcacggccat cgtcggcacg gccgtcggcg ccgcggcggg cgccctcgcc
    63481 ggcggtcaca acggcgccgg tgctggcgcg gccgtcggca tggtggccgg caccgccgct
    63541 ggcgcctccg acgcccgcta cgccggcgcg gacgcccagc gccgttacga catcgcctac
    63601 cagcagtgca tgtacgccaa gggcaaccag ttgcccggcg cctacggata cggcagctac
    63661 cgcccggcca gcccctacgg gacgcccccg cccgcctatc caagcgggca gcccggctat
    63721 tcgatcccgc cgcccaatta cccgcccccg ccgcccctgc gccgcgagtg acttcccctg
    63781 cgccctccgg cacaatccgg gcgcaggaga accgtgcatg aaatttgatg tcctcgtcgt
    63841 gggcgccggc ctcgccggtg ccgccctcgc cgccgccttg cgcggctgca gcctcaaggt
    63901 gggcgtggtg gaagcccgcg ccccgtcccg gcccaccggc tgggatccgc gcatctacgc
    63961 catcagcccc gccaacgtgg cattcctcac ctccatcggc atctgggggc acctcgacgc
    64021 tgcgcgcatg gcgccgatcc acgacatgga gatccacggc gacgccggcg gacgcctcga
    64081 cttctccgcc tacgactgcg gcctcggcga actggcctgg atcgtcgaat ccagcctgat
    64141 gcagcttgaa ctgtgggaaa ccatcaagcg ccagcacaac gtcaccctgc tgtgcccggc
    64201 cgcgccggcg gccctggccc tggacgagga cgccgcctgc ctcaccctgg cggatggccg
    64261 ccgcatcgag gcgcgcctca tcgtcgcagc cgacgggcgc gactcctggg tgcgccaggc
    64321 cgccggcatc gacgtccgca acacgcccta tgaagaaaaa ggcgtcgtcg ccaacttccg
    64381 ctgcgaaaag ccccaccgca acaccgcctt ccagtggttc cgcaaggacg gcgtgctcgc
    64441 ctacctgccc ctgccggcgc agcagatctc catcgtgtgg tccgccccgg acgacctcgc
    64501 cgaccagctc gtcgccctgt cgccggaagc cctcgccgtg cgcgtggccg aagccaccga
    64561 cgcgcgcctc ggcgccctcg agaccctcac cccggccgcc gccttcccgc tacgcctgat
    64621 gcgcgtggag cgcgtcgtgc agccgcgtct ggccctcatt ggcgacgccg cccacgccat
    64681 ccatccgctg tccggccacg gcatcaacct gggctaccag gacgcccgcg tgctggccga
    64741 acagctggcg gccctgccgg cctggcgcga ccccggcgaa ctggccgtac tgcgccccta
    64801 cgcccgcgcc cgcgccgagg aagccgccct gctccagtac accacccacg gcctcaaccg
    64861 cctgttcaag cccgccaacc cgctcctgtc cggtctgcgc aacctgggac tgaacctgac
    64921 caaccgtctg ccggtcctac gcaacgcgct ggtccgctac gccatcgccg gccgtttctg
    64981 acggtcgtcc gcccttaccc ggagattccc tggatgaagt ctgcctcgtt ccgccccctc
    65041 gccctcgccc tgctgctcgt caccggcagc gccctcgcca acgacgaggc cgccgtcagg
    65101 aagaatctgg acaccttttt cggcggtcag gccgtcgaca cggtcaagaa gacgccctac
    65161 ggcggcctct acgaagtcgt gctcaagtcc ggcgaactgg tctacaccga cgcggcggtg
    65221 agctttctgc tggacggcaa tgtcatcgac accaagacca agcagaacgt cacccaggcg
    65281 cgcatggccc agctgatgaa gatcgacttc gccaccctgc ccctcgacca ggccatcaag
    65341 caggtccgcg gcaccggcaa gcgcgtcatc gccaccttcg aggatcccaa ctgcggctat
    65401 tgcaagcgcc tggccaagga catggtcaat ctcaaggatg cgaccatcta caccttcctg
    65461 taccccatcc tcgggcccga ttccctcgag aagtccaaag gcatctggtg cgccaaggat
    65521 cgtgccgcgg catggaccga ctggatgacc gccggcaagg cccccgccgc gggctcctgc
    65581 gacaccgccg tactcgacaa gaatgtggcc ctgggccaga agatgcgggt gagcggcacg
    65641 cccaccatct tcaccgtgga tggcaaccgc ctgccgggcg cggtacccct cgcccagctc
    65701 gacaaggcca tggatcaggc cgctaccggc aagcgctgat ccggcaaacg cccgctcggc
    65761 aggccgacgc ctcgatcagg cggcggccgg ctcgcgcagc gggatctccg gcatcaccgg
    65821 tacgcagcgg cccgtcggcc gaccgagcag gcggccttgc agccagtcca cacccacgtc
    65881 ccgcgccacc tggagctgca gcggattctc gatccactgg cacaccgcca ccacgcccat
    65941 gcggcggaac tccgccgcca gccccatcag cacgcgccgc gccaggctca ggtggccgga
    66001 gtggccgatg atgctgctgt cgagcttgac gatgtcgggc atcagcgctt cgacgcgacg
    66061 caggtcggcc gagtggcgcc cgaaattgtc caaggccacg cgatggcctt cccgccggca
    66121 cgcgtcaatc gccgccgcca gcggcagcgc gctgccgaag ccgtcatccg ggaattccag
    66181 taccagctga tccgccagca gtccgcaacg catcagcacg ggatcgagca ccgtcaccgg
    66241 atgaacgccc cagtgcgccg ccgtgcacgg atggacgttg agggacagga agcccctgcg
    66301 cgccggctct accagaaagt tgagggcatg caggtgacgg cacaggcgat ccacctggat
    66361 caccgcctcc acatgctcca acgccaggaa cagggcctgg gacggcagcc ccgctggccc
    66421 ggcctgcacg gcgaggcgcg cctcgtgacc aatggcgtgg ccactgcgtg catccaccac
    66481 cggctggaat tccgatccca gcagccagtc gcccacacgc agcacggcgc gcccctccac
    66541 aagatcgagc cgaccttcgc ccccgcgcag ggcgtgacgg tggttgagat ggcggacgag
    66601 ttcggtgagg ggcatgaagg tctccgtgaa ggccggctga tgacacgggc acgagcctac
    66661 tctccggcca tgacgccgga tcgcggcaga accatcaatt tacgaaaagg atgttacagc
    66721 gatccgttcg ctgcccgcgg cacctgatcc cggggccaca gcacccacat ttcgcccttc
    66781 tgcttcatcc ggccggccag ggcaccggcc tcggcgccga agccccagaa gaagtccgca
    66841 cgcaccccgc ccttgatcgc cgtgcccgtg tcctgggcca gtaccaggcg ctgcaggggc
    66901 tgggcgctgc gcgggtgggt ggtggccagg aacaccggcg cccccaaggg cacactgcgc
    66961 gggtcgatgg cgatgctgcg accgtccgtg aggggcaggc ccagcgctcc ggccggaccg
    67021 cccggcgtgt tgggcacctc gcggaagaag atgtagctcg gattggcgcc gagcatctcc
    67081 ttcagccgtc cgggattggc cctggcccag cccttgatgc catccatcga cgcctgatca
    67141 aggcgtagct cgcccttgtc cgccagccag cggccgatgg actgataggg atgaccgttg
    67201 gtgtcggcgt aggccagccg caccagcccc ccgtcgggca gctgcacccg ccccgagccc
    67261 tgcacctgca tgaagaacag atccaccgga tcggccgcgt agagcagcac cttgccggcc
    67321 agcttttccg gctggcggtc cagctcggcg cgggagtaat aaggcaccac cttgcgcccc
    67381 accaggcgcc cgcgcaggcg atagggcttg agatcgggat aggcgtcggc caggtccacg
    67441 gtgatcaggt cgtccggtgt cgcgtgaatc ggccaggcgc aaacggctgt gcgggtcctg
    67501 cagccctgga tcagcggttc gtaatagccg gtgatgaggc cttccttcgt accatccgga
    67561 ttggtgaccc gccagggcgt caggcggctc tcgaagaagc tgcgcgcggc cgacgccgag
    67621 ggcttttcac cgagcccctt cgccgcgtcg cagacccctt gccaggtggc ctgccgaccg
    67681 agactgcggc acgactggag aaaggccggc caggcctcgt cgtgggcgtc gctgccccag
    67741 ccgggcagat cgcgccagtc tgccgccagc agcgggcgcg ccaggggcgt ggtgggcgcc
    67801 gcggccgccc cgggtggagc cgggacggca ggggccgccg gcacggacgg cggcgcgggc
    67861 cggggcgctt cgacggactt gggggcgctc gcgcacgccg ccagcagcag gactgccgag
    67921 gcgggtgcca ggaggcggga cagggaagct cggtccatgg atggggtaca ctcgcgatcc
    67981 ggatattcgg gctgcccgag tatataagct cactccccag gacgctccat gtggttgatc
    68041 ggcctcgaac tcggcgttgt cgccgtcctg ctcctgatcg tcgccctggc cctgcgcgcg
    68101 cccaaggaca aaaacgacga cccgcctccc catgactgac ctgaacgatc tcatcgcccg
    68161 cgccgggcag ttgctcgacc gcctcgaagg cctgctcccg gccgcgccga ctgctcccga
    68221 ctggcacgcc gccgtggcct tccgctggcg ccgcagccgc agcgccgcca ccctccagcc
    68281 ggtgctgcgc ccccacgcga tccgcctcga cgacctgcag gacatcgacg acaagaaggc
    68341 ccgcatcgtc gccaacacgc gccagttcct ggccggcaag cgggccaaca acgtgctgct
    68401 caccggcgcc cgcggcaccg gcaagtcgtc cctggtgaag gccgtgctca acgaattcgc
    68461 cgacgccggc ctgcgcctca tcgaggtgga caaggacgac ctgatcgacc tgccggacat
    68521 cgtcgaactg gtgcagggcc gccccgagcg cttcatcctg ttcaccgacg acctctcctt
    68581 cgaggacggc gagcccgcgt acaaggccct caagagcgtg ctcgacggct ccgtcgccgc
    68641 ggtgccggac aacgtgctga tctacgccac ctccaaccgc cgccacctga tgcccgagta
    68701 ctttgccgaa aacgcggcga cgaagcacaa ggacggcgag gtgcatcccg ccgaagcggt
    68761 cgaggagaag atctccctgt cggagcgctt cggcctgtgg atctccttct accccttcag
    68821 ccaggacgag tacctggcgg tggtgaatca ctggctgcgg gagttcggcg tgcccgccga
    68881 ggccgtcgcc gacgcccgcg gcgaagccct gcaatgggcg ctgctgcgcg gctcccgcag
    68941 cggccgggtg gcctggcagt tcgcccgcga ctacgccgga cgcttcgacg catgagcgcc
    69001 tcgcccccgc gcaagatcgt ccacgtggcc gctgcggtca tcacccgccc ggacggctcc
    69061 tacctcctcg gccagcgtgc gcccggcacc ttctaccccg gctactggga attcccgggc
    69121 ggcaaggtgg aggccggcga aacgccccgc gacgcattgg tccgcgaact cgccgaggaa
    69181 ctgggcatca ccgtggagac cgcgtggccg tggatcgtgc gcgagcacgt ctacgaacac
    69241 gcccacgtgt ccctgcactt cttcgaggtc cctgcctggc acggcgagct gacggacaag
    69301 gtgcacagcg ccctcgcctg gcaacacccg gacgccgttg acgtgacccc tgtgctgccc
    69361 gccaacggcc ccatcctcaa gggcctgcgc ctgccccgcc ggatgggcat cacccaggcc
    69421 ggcgcagtgg gcgtcgaacg tcagctgaag cgtgtggacg cggccctcgc cgcgggcctg
    69481 cgcctggtac agatccgcga accggagctg gacgccgaga tgctcgcctg ctttgcccgc
    69541 gaggtcgtgg cccgttgtca cgccgtcggc gccctcgcgg tgttgaacgg cgacgcggaa
    69601 ctcgcccaca cgctgggcgc cgatggtgtc catctgcccg cccggtgtct gatgagcctg
    69661 gcggcccggc cggatctgga ttgggtcggc gcgtcctgcc acacccgcgc cgaactggaa
    69721 cgggcggccg ccctcgggct cgactacgcc gtgctcggcg cagtgcaggc cacggcgacc
    69781 cacccggagc ggaagcccct gggctggagc ggcttcacga cgctcgccgc cgggctgccg
    69841 ctgccggtct tcgcactggg gggcgtgcag cgggatgacc tggaaacggc ccgcgccgcc
    69901 ggtgcccatg gcatcgcagc catccgtgct gcctgggaca cctgaaaccc ggggacgcgg
    69961 ccgcgctgcc gggcccgcgc cgcgtcaggt gcgcgtgagg ggatcggcgc cgtcgtcgag
    70021 cggcgtctca cccgccaccc ggtaggtgtc ggacgcccac gccccgaggt cgatcttgcg
    70081 gcaccgctcg gaacagaacg gccggaaggc gttctccggc acccacagga cggccttgcc
    70141 gcaggtcggg catttgactt cacgggaacg gaccggtgtg ctcacagatt gcagaacgtc
    70201 agttcgaagc gcacgtccgc ctcggactga cgcggacgga cgacggtttc ggcgtgcatg
    70261 aagcgaatgt tgagggcgta cttgttggcg ctggtttccg gcacgtaggg atcgccggaa
    70321 ctcaggcgga tgcggatcat ctgcacggtg cgcccgccca tggtctgctg atacgctccg
    70381 cgcgcggcca caagctcttc gggtcggccg ctggcgcgca gcagacgcaa cacgatggcc
    70441 aggccgtcgc ggatgggcag caggggctgg acccaggcca ccagatcgcg acgacggaac
    70501 tccacgtcgc ggttgagcca atggtgatac gagggcaggt cgaactcaca ggcgccgccg
    70561 ggaatcgccg cacgactgcg gatgttcatc agccattcgt tctcgcgcag gtactggccg
    70621 gtcttgccgg ccatggcgag cagggacgca gaggcctgct cgatctcgta gagggcgccg
    70681 gagagggcct cttcggagat ctgcggattg ttgcggaagg acaggaggat ctggcgctgg
    70741 cgctccagtt cctgcaccag atcaaccttc aggtcggcgc ggctggcgac ttcgaggatt
    70801 tcgaacaggg tgacgagggc gacgtggtgc tcaagggagc cgtcggcccg cacgaagtgc
    70861 atcaccttgt cgaagagatc ctccagacgg aggagcgtgc ggatcctctc gttgagcgga
    70921 tattcgtagg agatcacgcg cgcgcctgaa agattggagg aattgggcag tctgaccggc
    70981 acggatcata tcacagcacc cgaccccgcg cggcgcactg gacatattca ccgtggagcg
    71041 ccgcgacccg cgcctgcagg gcctccagcg agccggcgtt gtccaccacg tcgtccgcag
    71101 cggcgaggcg cgccgcacgc gtggcctggg ccgccatgat ggcgcggacc tggcgttcgt
    71161 ccagcccgct gcggcacatc acccgttcca cctgcagtgc ttcggggcaa tccaccacgc
    71221 acagacgctg cacgcgctcc cgataggtgc ccgactcgat cagcagcggc accacgagca
    71281 ccacgtaggg tgcgaccgcc acggcacact gacgcgcgct ttcgtcgcgg atcaggggat
    71341 gcaggatggc ctccaggcgc ttgcgcacgg aagcatcctc gaacgccagg gcgcgcatgg
    71401 cagcccggtt gagggcgccg tccgcggcga cgatgccctc accgaaagca gcacgcaggg
    71461 cgggcatcgc cgcaccctcg ggcgccgtga gggcatgggc aatgcggtcg gtatccacga
    71521 cggccgcccc cagcgtggcg aagcggtcag ccaccgcgga cttcccgctg ccgatccccc
    71581 cggtcagccc gacaacgaac ggcccactgc tcatggcttg cacccctgac gactgacacc
    71641 cttgacgtga tcggacagat cgctgacgag ggtcgccagg ctgtcgctgg gtccccgcac
    71701 ctcgagacgc accagggcgc tgccacgggt ggcgatctgg gcactgcgca cacccttggc
    71761 ccgcaggact tcgagctggc ggttggcagc gacctcgctc ttgaacagcc cgagggagat
    71821 ggcgaactga ttaggcccgg cgtcgggcac gatgtagagg tcatccacgc caagcttgcg
    71881 cacctccgcg gccttgcgat cggcgccatc cttgctgccc ggggatggaa tgtagaccca
    71941 ccaggacgac ggctgctcgc tgcggctttc gttcaatttg agcccgccgc cgaccttgcc
    72001 gatgcgtgcc gccagttcct gggcctgatc cacacccagc ccggcaaagg ccacacaggc
    72061 gggcggcgca gaggtgtccg tggcaggcgc ggattcgcgc tgcgccggcg cggccggggt
    72121 cggaacaggc ggctcaggcg aagtccgggg tgcctcggcg atcgcttccc gcggccgggg
    72181 cgctggcgga cgggcatcca ccgcgagctt gaggcgctcc gcgttcagct ggttgctgag
    72241 ccgttcaggc tggccatcgg aatcgcctcc gcccagccag cccttccaca aggcgagcgc
    72301 cagcaggttg gcgaagatca ggacaatgac ggcgacacgc aggggaacca tggacacaag
    72361 cctaacgtaa cggtgcggca cggagaagca tcggactcaa cttgcatcat ttgcccgatg
    72421 tcaggcgggc caggccttcc agcaccagat tgtcgacctg acggacggga cagccgagct
    72481 gcgggcccag atcggccgcg gcaccgccgt tgagcagaca gaccgcgtca ggcgcatcgg
    72541 ccaccagccg gaacatgcgt tcgatggccc ctgcctgggc atgcaggcag ccactgacga
    72601 tcgcgtcggc ggtacgccgt ggcgtcgcgt cgaagcgccc gtcggcgaag ggcaggcggg
    72661 ccgtgtcgcg ggccagcgcc cgccgcatca attccagccc gggcaggatg atcccgccgc
    72721 ggaacacgcc ttcggccgtc agcacatcca ccgtcgtcgc ggtaccagcc gtcaccacca
    72781 gcgcggcacc ggcatgcagg gtgcgggcac cgatcagggc ggcccagcga tccgggccca
    72841 gttgggtcgg atccagatag gcgttgcgta ccccgccggc ttcaatgccc ggcaccagcc
    72901 atccgggcgt ccgcttccac gcggcacaca ggcgccccac cgcgagggcg acagacgccc
    72961 cggccacatt ggcgcccatc acccgtgcag gcaccggctg aacagccagg gccgcctcca
    73021 gcgcttccgg cgtggcggtc ggaacggcgc cccgcgcgtg ccagacagcg ccatcatgca
    73081 gcccccactt gacacgcgta ttgcccgcat cgatgagcag gttcatgcga cgctccgcag
    73141 gctcacttct ccggagagca ccctttgaag accggaaccc gtattcagca gcagggcgcc
    73201 atcctcgtcc acgccgacgc acgtcccttc ctgcgagacg gtttcgccga gaatgcgcac
    73261 cggcagaccg gcgtgggcgt tgcgttgctg ccaggccccg cgcaacgccg gaaaacccgc
    73321 cgcggcatac agttcaagca gaccgcggaa ttggtcaagc agacacccga ggagccgcgc
    73381 ccgcgtcggc ggcgccgtca ggcaggcggc cagatcggtg acgccgcctg cgtgggggat
    73441 gtcggcctcc ggcggcaggc gcaggttgag ccccacgccg atgaccgcgg cgggggtccg
    73501 tccgcgcccc ggaatgagct ccaccaggat gcccgcgagc ttgcgtccgt ggatcaggac
    73561 atcgttgggc cacttgagct gcaccccttc gacccccaac tcttcgagcg cccgcgccac
    73621 tgccagtccg gccaccaggg acagcccggc cggcaccggc gcgcccggtt cgaaccgcca
    73681 cagcaccgag aaggtcagac ccgcgcccgg ccacgacagc cagttgcgcc cacggcgtcc
    73741 gcgacccgcc gtctggcggt ccgtgacgag gacatggatg cgtccgtcgt ccgccggcgg
    73801 gtcatcgagg aggacggaat tggtggaggc gcactcgggc accacgacca cggaaaaggc
    73861 cgcggcgtgg tcgcccagga cggcgagcac cgcgtcgcgg tcgaggggtt cgcaggacaa
    73921 ggaatgcaag ggtcacccgg caggcatgat cgattcgcca ttatcacgga tttccttgcc
    73981 gcaacttgat ggccggacgg accggctgtg gtctgccgcc gacgggcggc agtctggcgg
    74041 ggccggatcg actcaggcgt cgcgacagaa ttcggtcgac cgaccatgcg gacaagcctc
    74101 gggaccgctg tccgcgggtg acgggcaccc gcggacagcc gcgcccctgc cgctcaggcg
    74161 tgctcgccct cttccagtcc cagttcctgg atcttgcggg tgatggtgtt gcggccgata
    74221 ccgagcagct gtgccgcctc gatgcgccgg cccccggtgg cgttgagggc gcgccggatg
    74281 aggatgcgct cgaagtcccg cgacaggcgg tcgaacactt ctcccggatt ctgcaccagg
    74341 gcccgatccg cttccgcggt cagggcgtcc acccagttgg ccggctgctc gcgggtgggc
    74401 tgctccttca actccggcgg caggtcggcg acttccaccg tctgccccgg cgccatgacg
    74461 gtgagccagt ggcacaggtt ttcgagttgg cgcacattgc ccgggaaatc caggctctgc
    74521 aggtacttga gggtggcatc cgagaggcgc ttgggctcca cgccgagctc ctgcgccgac
    74581 agggcgatga agtgcttggc cagcatcggg atgtcctcgc gccgctcgcg gatcggcggc
    74641 agacgcaggc ggatcacgtt gagccgatgg aacaggtctt cccggaacag gccctgacgc
    74701 acccgctcct ccatgttttg gtgggtggcg gcgatgacgc gcacgttggc cttgatgggc
    74761 tgatgaccgc caacccggta gaagttgcca tcggacagca cccgcagcag gcgcgtctgg
    74821 agctcggccg gcatgtcgcc gatttcgtcg aggaacaggg tgccaccctc ggcctgctcg
    74881 aagcggccgc ggcgcatggt ctgggcaccg gtgaaggcgc cgcgctcgtg gccgaacagc
    74941 tcggattcga ggaggtcgcg gggaatcgcc gcggtgttga tggcgatgaa gggcgcgtcg
    75001 cgtcgcgggc tgtgacgatg cagggcgcgc gccacgagct ccttgccggt accggactcg
    75061 ccgttgatga gcacggtggc gtgggactgg gacagccgcc cgatggcgcg gaagacttcc
    75121 tgcatggacg gcgcctggcc gaggatctcg ggcaccgcgc tga
//
LOCUS       DAOQMM010000006        66224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_63_length_66224_cov_45.880654, whole genome shotgun sequence.
ACCESSION   DAOQMM010000006 DAOQMM010000000
VERSION     DAOQMM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 66224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66224
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_66224_cov_45.880654"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(544..1032)
                     /locus_tag="PLH85_01900"
     CDS             complement(544..1032)
                     /locus_tag="PLH85_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63338.1"
                     /translation="MKVKFACVLAMLSFEVINAQAADIGVSISVGQPGFYGQLNIGDF
                     PRPALINARPIIIESGPGYMEPVYLRVPPGHQKNWSRYCRNYGACGRPVYFVTDRWYN
                     NVYVPAYRERQGYPEPRDRGYDPHPDHDHRDHGWRDDDRRGGGRDDHGGGHGHGHGRG
                     RD"
     gene            complement(1306..2877)
                     /locus_tag="PLH85_01905"
     CDS             complement(1306..2877)
                     /locus_tag="PLH85_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNB63339.1"
                     /translation="MSAAHEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEIR
                     ARKAARHATSDWMELEKQRGISVTSSVMQFPYRDCVINLLDTPGHEDFSEDTYRTLTA
                     VDSAVMVIDSVNGVEAQTIKLLNVCRLRDTPIVTFINKLDREGREPIELLDEIESVLG
                     IQCAPMTWPIGMGKRFRGVYHLYNDTVQFFDPQAEKGTSEIIEGLDNPRLDALIGDQA
                     DELRLDIELVRGASHSFDRDAYLGGKQCPVFFGSAVNNFGVQSLLDAVVDLSPAPLAR
                     PAKTRQVQPDEDKFSGFVFKIQANMDPKHRDRIAFIRVCAGRFERGGKLKQVSTGKSL
                     AINNAITFMAQDRNTTDEAWAGDIIGIPNHGTIHLGDTFTEGEDLQFTGIPSFAPEHF
                     RRAQIKNPLKMKQLQKGLEQLAQEGATQLFRPTTTNELILGAVGTLQFDVVAHRLEFE
                     YGVDVMFESCAYATARWLRGSDADLKALADKSPANMALDGNGDYVYLAPNRVNLQMAQ
                     ERFPNIRFLETREIY"
     gene            complement(2981..3463)
                     /locus_tag="PLH85_01910"
     CDS             complement(2981..3463)
                     /locus_tag="PLH85_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB63340.1"
                     /translation="MKARARLASDLPDGVVITPLDDGPGPLREAAAAILLASFDDPER
                     YGPARIQSQLDSAPLPYYRRFFIARRDDEVLGVGGIKAADWASDTHLLYLSAVAEHAR
                     GQGIARALVMTRVAWVMETFEHGRVLVSTPRPRRFARFGFRAAGRLESAGRRLMLLEF
                     "
     assembly_gap    3759..3858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3859..4941
                     /locus_tag="PLH85_01915"
     CDS             <3859..4941
                     /locus_tag="PLH85_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB63341.1"
                     /translation="ARDSATWFAVGILVVACAAMGVISLMTIRAIAGRLEEANALAAR
                     VAAGDLTGGKASPNSGSDEIGQLLASLDAMRVDLAQTISGIATNAENVVGYAAQLSSA
                     AQQVSVSTEQQSRNTAAAAAAVEELTVSIDHVGSSAGDARVRAADAGSKAVDSARGVD
                     TAADKVGQVANQVETTSQQIQTLSQQVQQIDKITTVIREVADQTNLLALNAAIEAARA
                     GEQGRGFAVVADEVRKLAERTTSSVQEISNVVGAIQQGAVGAVNSMQVSRDLVAAVVG
                     SARTASESMSEIRESASTMSGVIESISDALQEQRAASTELARNVESIAQMSEENAAAV
                     GSVAETAHRLSSVSDALKSNVARFRV"
     gene            complement(5036..6160)
                     /gene="pqqE"
                     /locus_tag="PLH85_01920"
     CDS             complement(5036..6160)
                     /gene="pqqE"
                     /locus_tag="PLH85_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HNB63342.1"
                     /translation="MEALLIPPRPGPLWLLAEVTYRCPLHCPFCYNPVDFAQAGPELS
                     TDDWLHVIRQARALGAAQFGLSGGEPLLRDDIEILVAEARRLGFYTNLITSGVGLTEA
                     RLAALKDAGLDHIQLSFQDATREANDLITNTRTFDLKRRVAALTKAAGYPTVINCVLH
                     RGNIEHLDRIIEMAAEIGAEYLELANTQYHGWAYLNRAALMPTAAQLAEAERVTERWR
                     VRLGKAMRILFVTPDYASTAPKKCMNGWGEVFLAITPDGLALPCHTARMLPGLDFPDV
                     RAHDLAWIWHDSPLFNRYRGTAWLPDPCRSCPDKEADHGGCRCQAFMLTGNPDATDPV
                     CPKSPDHALIQTALQAAEQPTAQPLVFRDPKQSAALARPF"
     gene            complement(6171..6455)
                     /gene="pqqD"
                     /locus_tag="PLH85_01925"
     CDS             complement(6171..6455)
                     /gene="pqqD"
                     /locus_tag="PLH85_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNB63343.1"
                     /translation="MHSTPAPSSHPRIGRLFRFQWEPAQQAHVLLYPEGMVKLNRSAG
                     EILARCDGTRSVAEIVAELEAAFQASSLTADVEAFLRLAEERSWIDWTPT"
     gene            complement(6476..7198)
                     /gene="pqqC"
                     /locus_tag="PLH85_01930"
     CDS             complement(6476..7198)
                     /gene="pqqC"
                     /locus_tag="PLH85_01930"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HNB63344.1"
                     /translation="MNTPVKPAPWTPAEFEAQLRDKGAAYHIHHPFNVLLNTGGARPE
                     QIRGWVANRFYYQIIIPQKDAAILANCPDRGVRREWIQRIHDHDGHGDDAGGIEAWLR
                     LGQAVGLARDEITDLRHVLPAVRFACDAYLNFCRQRPWQEAVCASLTELFAPTIHKQR
                     LANWPEHYPWIESDGLQYFRNRVTQARRDVEQGLAITLAYFDTRAAQQRALDILKFKL
                     DILWTMNDAMAQHYGLAGGRPA"
     gene            complement(7195..8112)
                     /gene="pqqB"
                     /locus_tag="PLH85_01935"
     CDS             complement(7195..8112)
                     /gene="pqqB"
                     /locus_tag="PLH85_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HNB63345.1"
                     /translation="MRVHVLGAAAGGGFPQWNCNCRNCRGLRDGSLSARRRTQSSIAV
                     SADGARWTLVNASPDILQQFQAFAGTWPTDGLRGCGVESIVLVDAQIDHTTGLYMLRE
                     KGSPWPVWTTDPVYEDLTRGNPILHLLDHYCGIDRRAIPLDGRVFRPQGAEALEWRAM
                     PLLSAAPPYSPHRGQPVPGDNIGLTIRDPASGRRVFYAPGLAEVDDAVFDAMAGADCV
                     LVDGTFWTDDEMIRLGLSTKRARDIGHLAQSGPGGMIEQLARLPRHVRRVLIHINNTN
                     PILDEDSPERATLTAAGIEVACDGMEIAL"
     gene            8458..9387
                     /locus_tag="PLH85_01940"
     CDS             8458..9387
                     /locus_tag="PLH85_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB63346.1"
                     /translation="MSPSPACAVPAAFYLDVSDGHRLFVATAGAPGGEPVLVLHGGPG
                     SGCSPRLAELYDPARHWLVMPDQRGAGRSAPAGACHANTTAHLIADLERLRRHLGIAR
                     WRVTGGSWGATLALLYAAAHPAAVSGVVARSVFLPGAGNVAAFFAAGGDVPAERVALA
                     DAAGCPAGGDVLAALARRLDDPEPARAVRAAVAWAAYERALAEPEAPPQADLDAAAAE
                     CLVRKYRVQAHYLQAGAFVTEAGFLDACASLPGVPVTLIHGRLDRVCPPDNARRVHAR
                     IPGSRLVWVDGCGHAAFHPGMVAAWRQALGGAG"
     gene            9722..10333
                     /locus_tag="PLH85_01945"
     CDS             9722..10333
                     /locus_tag="PLH85_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB63347.1"
                     /translation="MSTPPRSDPGRRERKRLLQLDHLADTAWALFDAEGYEAVTMERI
                     AAVADVAKGTLYKHFPVKEALLRHRFHRELREGLERLQPVVLAEAPGAARLRRFFDLS
                     AQWSERHRETLLPYVRFRLAEPCHGDDSQRSGLAQVFTGLIADGQGAGGFRTDIGAEV
                     LANYLEFLYLASLLRWLTGATPVLQAEFERMLAVFLGGLEVRS"
     gene            10330..12822
                     /locus_tag="PLH85_01950"
     CDS             10330..12822
                     /locus_tag="PLH85_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNB63348.1"
                     /translation="MNGALLNPEEVRVTDATVTGGKGRQLAQLRHFGLPVPDFAVLPA
                     AWSEGRGEAFPAALVTALHDGLAARGWLEQPLAVRSSAVGEDSAAASFAGIYTSCLNV
                     QGLDGVVAAVQTVWASLDSDTARAYRARMGHAGPARMGVIVMPLLAAEASGIGFTCDP
                     LTGREDRLVIHANRGLGETLVGGETAGDEYVFAEDVEDAWRLIECRPGPKTCMSVPAP
                     GGGTRRVALPDAAAAPLFDTERAEALAALLRDAAVAMDFVAPFYDLEWVWDGARFWLT
                     QARPVTRRPRHAYGPLQAQPAIWTRGNTCEVMPEPLQPADWNFSRRGVNDLLVQGYVL
                     AGFPVLAGAQRAGLFEGRLYLEASLLQWEAWDAIGMKPERLNALMGGHQPAIDVTPPG
                     GRERLRRALTTLRYLVRAPALRRRGEAEIAAMHALARQVRQGPPPRDRDALRRLLYTV
                     SRPGRTSIGAFFLQGSGGASLSFLVERLEHFFPGEGEALGAALLTGGTPSVTAQQGYD
                     LLRLARLARAAGFPAQVPAQGDYARAFAEFMEVHGHRGHYETYMHSASWRERPERLLE
                     QLPALAEVDEAALRARQQDAAAAAWQRVRREVPFWHRPLLRSLVKAARRECNQREAAR
                     SGLIALLEVCRMAWRLAADLLRADGALAERDDVFFVLPSEAERAVDGLVPPAGLRARV
                     AARKALYEAWQAVTPPEYLCIAPAGEWLAAPAWPAPPHRPGEGWRGVATGTGRARGRA
                     RILRHPSEGSRLQAGEILVAPSTDPGWTPLFLKAAGLVVETGGYMSHGAIVAREFALP
                     AVVNLPGILDGLQDGDEVEVDGRSGEVRLIRP"
     gene            complement(12868..13752)
                     /locus_tag="PLH85_01955"
     CDS             complement(12868..13752)
                     /locus_tag="PLH85_01955"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HNB63349.1"
                     /translation="MARPILEHTMALPRPAPVLRLAALLPLAVLCGPTLAPAQDAAPC
                     SPARLAAIERQSQPLDRSGHGPDRGSAEWASAVEWRLGLRGRDDLPARGSRAWCERIE
                     AALRQRDEQARLYASRPGPTFSCRGVQPGSIPDLVCADTALGTLDRDLQRVYAQAART
                     AAHERPPVLKAEQRGWVRGRDDCWKSDDRRACVADAYVTRIAELQARYRLVAHRGPFR
                     FSCNGQPADEVLITHFQTDPPTLIAERGDQTSLMFLQPAASGTRYVGRNESFWEHQGE
                     ATVVWGHGAPEMRCILDR"
     gene            14002..15177
                     /gene="ypfH"
                     /locus_tag="PLH85_01960"
     CDS             14002..15177
                     /gene="ypfH"
                     /locus_tag="PLH85_01960"
                     /inference="COORDINATES: protein motif:HMM:NF008525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNB63350.1"
                     /translation="MLPSSSIPVALPAHADRLVLLFHGVGSQPAAMVPLGERIARALP
                     AAAVVAVAGPQPSGTAGGREWFSVAGVTDANRPGRVAEALPTLLDAVAEWQRRTGVAA
                     EATVLVGFSQGAIMALATLARADAPARTVVAIAGRLAEPPAQVAPATTVHVLHGEADP
                     VMPIGLAEAAVARLAQLGARVSFDRFPGLGHGISEALAAGLVARLLGDGAPAGEVGSV
                     SALAIRLEAEQAIVSRPAAQGGEEVRSLALGPATLVRRCLHHTPPTAGELEAAIDLIE
                     PQIMALGLHLPAGAVLRLSGDWPADLQRPAPGLHRDAVEGLFSRLAAIAHGRPAASEG
                     LPADPAYFAAALLLRELLHHLGAVRAEAAAPAQGGGPQRSANTVTPAGPEPLKKADT"
     gene            complement(15114..16706)
                     /locus_tag="PLH85_01965"
     CDS             complement(15114..16706)
                     /locus_tag="PLH85_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB63351.1"
                     /translation="MHSTLRLTLLTLALATGGSAVASDGPTTADFNAAADDTSNWLYA
                     THDYSGQRYSRLRQINRSNAARLQAVCMYRSADAGPAQTNPVVYDGTMYLSIARSIVA
                     IDAATCREKWKYTWEPKGNELSPTNRGVAIKDGKVIRGTADGYLIAVNARDGSLAWSR
                     QIASSTGQQYLSMPPLIFEDMVIYGPAGADFGQKGWVGAFNLSDGTERWRLPFIPDPG
                     QPGAETWSNPEALKYGGGSLWTPVTLDAKAGTLYVAVGNPAPDFYGDARPGNNLFTNS
                     LLAVDVRSGKMLWYHQAVPHDIRDWDLTHAGPLFSTTVEGVRRNVIAASGKDGLLRLI
                     DRDSHEVLYTVAFTTQENVDVPLTVEGTRSCPGLLGGQEWNGSAYSPRTNTLFVPAVD
                     WCTTFKKAATTPEFKREEHYYGGSIVMDPFDKARGWLTALDASTGKVRWRYASGTPML
                     AAVTATAGDVLFTGDFNRDFLALDATSGKVLYRFNTGGEVAGGIVTYAVGGRQYVAAV
                     SGYVSAFFKGSGPAGVTVFALR"
     gene            16896..17261
                     /locus_tag="PLH85_01970"
     CDS             16896..17261
                     /locus_tag="PLH85_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB63352.1"
                     /translation="MKREHAEPVPQPPEGCPLERYLHVVSGAWVPRILWFLSFGPRRF
                     GELRRDIGNISAKVLTAKLRDLEGAGLVARTETRGQTRQVAYALTERGRIFEPVFDAM
                     VEVSHRLAELEAREAASKD"
     gene            complement(17308..17796)
                     /locus_tag="PLH85_01975"
     CDS             complement(17308..17796)
                     /locus_tag="PLH85_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63353.1"
                     /translation="MIRATLALRAALAALFIAAAPLPGHAATELQPLDRHAARSLTDP
                     RSHTRPTAIALWSAECEHCKKNLAALAELARRHPQLRIVTVEAEPATADSAPLLARTG
                     LTRDERYAYGDESPEALAHALDPRWRGELPRTYLFDGQGGSLTRSGVLTAADMRSALK
                     LK"
     gene            complement(17793..18974)
                     /locus_tag="PLH85_01980"
     CDS             complement(17793..18974)
                     /locus_tag="PLH85_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNB63354.1"
                     /translation="MKKILPILFPLLAALTLPAAAHEGHDQGPAARPARIELATAAAF
                     GPDGQLHIVAKDGAHLVWRHSPDLGATWSAPQHVNMQAESIAADGDSQPRIAFNRDGE
                     LLVTWTKPLPKPYTGEIRLARLPRDGSAFDAPITVHRDQAEITHRFQNLIVAPDGRVT
                     VVWIDKRDQEAARLAQKPWRGAGVYSAISDDGGRSFQPERKLAEHSCECCRIALALDP
                     DGTPVALWRHVFAPNERDHALARLKADGTPEAVQRATFDRWRVDACPHQGPALAIAAD
                     GVRHAVWFNQRDGEGRVFYGRPTSAGVNGQRPVGGPAAAHADIAVLGRRVVLLWKEFD
                     GERTRLRAQRSDDDGRHFDPPQELDATAGASDQPRLLTRDGRAYAFWRTADAFRVVPL
                     P"
     gene            19275..21488
                     /locus_tag="PLH85_01985"
     CDS             19275..21488
                     /locus_tag="PLH85_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB63355.1"
                     /translation="MKTRLRPLPAALMLMTLLPAALSARAAADSVELAPVVITASRSE
                     TRVEEMPLHTTVIAREDIERSPARSLDQLLRSVPGMNFTGVPASQSDPTGHQTRMRGL
                     GNAKVLVLLDGVPIHDPFYMTTQWFKVPLSNVERVEIVRGGNSSLWGNMAVAGVVNIV
                     TRRVQDDAGEVSLSAGSYGTWNVALSKNVAVSEALGFNLTVDDYQSNGYQTTPAEFMW
                     RFPHKQPVAVENRNVQLTAYFRPSADLNGWLRLGYHIQDQDISYQYGSNVQKSPDLAG
                     SLTRRFDDKTRLTASAWAQQVKFEKYNGSTCYYQGGTSCLNSNSATLTPAKVNDQVVE
                     FYTQYGSQRYREQGTSVVFSQDLAGRFSSYQLGVDYRALRAEDAEWFYTTPTSPTAPQ
                     GNLNSTTWGKGRQTFAGLFGQGKFFPVDPLEVTLSARYDTWRIDERANLRTTAAGVAS
                     GGALQDGSKNAFNPSLALRWELSDALSLRSAVYRAFRAPGFNNLTRTFGTGTSTTIAN
                     PDLAPETLTGWEAGADFRRGPFNLGATWFHYNIKNMIATYTATAASAPAQVQVICGGP
                     TLPGCGGSAKYYTNDQDGRAYGIELVAGWQVRPTLSVDASYTHTETFLTRHGSIVTDP
                     LNVQLVATPRDVAAVGTTWQPVERLSTYTELRYIGSMYLDTTSNGATARLQQGAIPLV
                     NASVRYAWDRRTDLFVSLVNLLDREYSENAYTINQPYNRTLSMPRTATAGVTLRF"
     gene            21623..22030
                     /locus_tag="PLH85_01990"
     CDS             21623..22030
                     /locus_tag="PLH85_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB63356.1"
                     /translation="MTAIRILPVLLVALLAACGKSEAPKTEAPPPAPAAAAPAPQAAA
                     PAADTADGGKGAELFKKTCAMCHQTGVAGAPKLGDKADWGPRIAQGKDTLYKHAIEGF
                     NGNKGAMPAKGGNPAFTDDEIKTIVDFMVSKAE"
     gene            22191..23021
                     /locus_tag="PLH85_01995"
     CDS             22191..23021
                     /locus_tag="PLH85_01995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB63357.1"
                     /translation="MDPIARTAFYCCGVRAADAASPRPICGDHLAARFMDDEARAVFA
                     RFARFRGPNLSNAARHRLVDDFLRAHLARHPDGLIVLLGAGFDTRAFRLPGGRWVELD
                     QPALLARKEHALPADTAPSPLQRVGIDFATQDLAGVLAPWAGHETAVVVLEGVSMYLT
                     DAAFAATARALHAVLPGHTLLCDLMDARFARRYGGGLRRAIRALGGDFAPDRADPAAF
                     VESLGYASRSVASIPARAVEHGTLGIPRWLFNTLLRSLRDGYRLHGFEARRSPASPSA
                     "
     gene            complement(23013..23501)
                     /locus_tag="PLH85_02000"
     CDS             complement(23013..23501)
                     /locus_tag="PLH85_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63358.1"
                     /translation="MKPQRTLVLLLSVVALSATAAVVNGGGYVAGDGFTLSEALAQAR
                     QEHPPGSFRPFWLLALDGAVGALARRGAGPDMATAVADIQRRGGTVYVCEQDLRAAGL
                     DSAELLPGVQAVRGWSDAESGIRNEDGASDAGADDAEQPLSPLRAVNRLCGSRPTPDD
                     SR"
     gene            complement(23523..24161)
                     /locus_tag="PLH85_02005"
     CDS             complement(23523..24161)
                     /locus_tag="PLH85_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63359.1"
                     /translation="MPATTVSASNVIADPTKTLQRFDLLDLEQYTQEKSYATNASKDF
                     HLFYVGRDDVHEILKHILSRVSVSLYMNMFGFDDEELNDILMQLANDPSVVMMITLDK
                     SQAGGVHEKRLLDSDAAKNPHAFNTYFVIGESATHQISHTKGFVADGRVGGEGSTNWS
                     ASGEGTFVVQGHAGGPGYKAQNNTQTIFTDPDTLSRFQAELIAEHLAARGKA"
     gene            complement(24364..24858)
                     /locus_tag="PLH85_02010"
     CDS             complement(24364..24858)
                     /locus_tag="PLH85_02010"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB63360.1"
                     /translation="MIRSPFAALGIAAFLAILPASAADPAPQPVPRNARCAVCGMYPA
                     QYPQWKAQIVFNDQSAAQFDSPAELFRFLHNMPAFDRRHSAADVGAVWVSDYASKAWV
                     DARKAVFVAGSRARGPMNDANLPAFSTREAADAHIRSQGGRALAYEEITREVVHHLAG
                     GHAH"
     gene            complement(24855..25226)
                     /locus_tag="PLH85_02015"
     CDS             complement(24855..25226)
                     /locus_tag="PLH85_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HNB63361.1"
                     /translation="MSLHPDLAARLDERFGTRFDAPVELKLDALLLRLDNGVILEARI
                     AAPDAYAIAWVWGDAELRIDTAPVHPELATFPHHVHGADGRLRADPLTVCGADPFDNL
                     VRVVDALLEDPLLGAPPGTAP"
     gene            complement(25237..25506)
                     /locus_tag="PLH85_02020"
     CDS             complement(25237..25506)
                     /locus_tag="PLH85_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63362.1"
                     /translation="MEPFYANLPPELALDVEQRARLLYALRGARDEVLARLGAGDADQ
                     ALACLRRGELPEHPAYDDILSARILADLHAQVRSDLATLLADSQR"
     gene            complement(25526..26059)
                     /locus_tag="PLH85_02025"
     CDS             complement(25526..26059)
                     /locus_tag="PLH85_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB63363.1"
                     /translation="MNIPRPLRRLGLAAGLGACVLLAACGDAARSGGATPVEITRDTY
                     CALDGMLLADYPGPKAQIHYDGQPEPEFFCDTTEMFAIYLKPEQARKVRAVFVQDLDK
                     TRWDAPQGAWIDARTAYYVVGSGKRGAMGPTIPSFGSEAGARKFAEAQGGQVHPFAAI
                     TPDMARLDGGALHDQKM"
     gene            complement(26056..26877)
                     /locus_tag="PLH85_02030"
     CDS             complement(26056..26877)
                     /locus_tag="PLH85_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB63364.1"
                     /translation="MSIDFRQIGTIAGKEFWDRIRNRWVLAVALVFTVFALVIAYFGA
                     AQQGAVGFRSIDVTIASLVSLVIYLIPLIALILGFDAIVGERERGSLDLLLTLPITRL
                     ELLLGKYAGLAAALAFSTVAGFGAVGLILSPQLDLNALFHYGGFMLSAILLGFAFLSL
                     AVMLSVFAADRTRASGLAIGTWFFFVLVFDLLLLGALVVTAGDYGGSIFPYLLLLNPA
                     DVFRVLNVFSLDEVRTLYGLATVFPEALSHPAVLGSVMFAWIVAPLALALWRFQR"
     gene            complement(26915..27829)
                     /locus_tag="PLH85_02035"
     CDS             complement(26915..27829)
                     /locus_tag="PLH85_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB63365.1"
                     /translation="MASTIVETRGVTRHFGTVQAVQGVDLAVQAGELFGLIGHNGAGK
                     STLFKMMLGLLPVTAGEILIDGRPVNGPDFRDTRRRIGYLPENVVLYDNLTGLETLHF
                     FADLKAVPRRDCAPLLDTVGLAPAAGRRVREYSKGMRQRLGFAQALLGTPRLLFLDEP
                     TTGLDPAAIREFYRILKDLREQGVTMILTSHILAEIQERVDRLAILEDGRVRAVGTVQ
                     ALREAMDLPVTLEIAGGAGLREQVARILAGLPVERLDETHDSLHIRLPREAKMPALAR
                     LATLGETLRDLHVREPSLEDVFFGYRGQ"
     gene            complement(27810..29132)
                     /locus_tag="PLH85_02040"
     CDS             complement(27810..29132)
                     /locus_tag="PLH85_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNB63366.1"
                     /translation="MSITPHACMFAPFFRHVRHAAACAATVLTALSGPGAPSAHAADW
                     RVQPGDSIQAAIQRAAPGDTVRVARGRYVENLRIDKPLNLLGEGRPTVSGGLRGDTLR
                     IAAPDVRVDGFIVTDSGADLGEQNAGIYIQPGAHRARVSHCDFSYTLFGLWIEKANDV
                     VIEHNLITGKRDLDSSQRGNGIQLYNTTGAQILANNISFVRDAIYVDVSHRAVFRGNR
                     MHHSRYGTHYMNSYHNLWEDNDTYFNRGGLALMEVRDQIVRNNRAWGNSDHGIMLRTI
                     QDAIVENNVVAGNARGFFIYDAEYNTLRGNLVVDNQVGVHLWAGSVRNIVERNDFISN
                     REQVKYVAARDETWGAKDGNHWSNYVGWDRNGDGFGDVPYEANDLVDRLSWRHPLVKL
                     LLASPAIQTLRLIAQQFPLLRAPSIVDPHPRMRPDHPDWRHWLGKHYR"
     gene            29377..29583
                     /locus_tag="PLH85_02045"
     CDS             29377..29583
                     /locus_tag="PLH85_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB63367.1"
                     /translation="MATGIVKWFNDAKGFGFITPDGGGEDLFAHFSAIQAKGFKTLKE
                     GQRVEFDVATGPKGLQAANIRPAD"
     gene            29892..32699
                     /locus_tag="PLH85_02050"
     CDS             29892..32699
                     /locus_tag="PLH85_02050"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNB63368.1"
                     /translation="MVKLKLRRVCAAVGAGLGGVCAASAIAAAVPETVTLNAVADSYV
                     DANSYNNYGKIVYFMVGSTKSAYLRFDLSSLADRDIRSATMKVRAQKPFSDTFSVMHA
                     VDNGWNEYSLGWFNRPYNVVSAQGALSTVATTNGGWATVDVTNAVSLTQGRPLTLQIS
                     GTNTTPLWFDTRESGNTPTLTVTSVPKSVTLTPVQDSYVDPAMPDTTFAGAPSMKADP
                     SGAEVIMRFDLSALTDRTVLGGTLHLKTPYGGPQDVQIYMPANADLQPNVLTYTTQPT
                     LLPDPVSAPAVAEPGGVAIDVGSILKGLNVRSPLSLVIRSSGSTLGIASMETATPPTL
                     EVLYRSVSITSTPLSSVPLKVGINIEATNYYGTEVPGIDLMKQGHTWYTQCYPWRDAL
                     CSAGKFSRSGGSSWDTLEQDVLDQDASGWVRSLPAPGDGATTGVNYTSIATLIPSSLS
                     DRYPGGRYIVRYDGEGTFSLTNSKATVTRNTALSSPGRAVFDIVGNPATAVGSAMNLS
                     ITATDPNRTGNYLRNIRIFPPGGVCSDDVTRYCNAQTGVPACSTGAICQDFETVYPAK
                     PFDPRFLANIKRFDTLRFMGYQSTNNAVSEAEWSDRTRPDHYTWIRTGYHLNPIENVV
                     DLGNTLHRNIWVNVPHRASDDYIYQLATAVKNRLSGDLKVYVEYGNEIWNTMFSAGSW
                     VEQQGLAEWPYAPDSAYVKRLQWQGKRTAETCDIWASVWGSDKSRVICVAGIQTGSAW
                     AAGYVLDCPLYAAAHGGIPCYQHNMKAVAVAPYFGGYIGDWSHKAAVESWTLDADGGL
                     GRLFAELFQGGQFTDSPPGGAIASALRALDTSVGLSRDRGLQTVSYEGGQHLTAVGAS
                     LQSPAIVQLLTRANRDARMGDAYKTYLAGWVGSGASLFTQWHSVGSYGVWGNWGLLEY
                     RDQPGSPKFNAVQTFLGTVGQ"
     gene            complement(32784..33203)
                     /locus_tag="PLH85_02055"
     CDS             complement(32784..33203)
                     /locus_tag="PLH85_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription regulator"
                     /protein_id="HNB63369.1"
                     /translation="MTVADPTELRQPPYGDTRGVARLSAEIIDHLWPWRRGSGGSGLA
                     RGAGITLALAATVAWILTALGHLSAGAIIGWWFGWSVLEVRVRHDAKPYVKDGPWWGR
                     RYRRANLMDLICYVSFKNLLIGAALFIGLKTAGLLVV"
     gene            complement(33200..35377)
                     /locus_tag="PLH85_02060"
     CDS             complement(33200..35377)
                     /locus_tag="PLH85_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB63370.1"
                     /translation="MFSLRTLAAALATAAACLLAGAAQADTYEVKLPPELFTSPRLCD
                     YAPCTDVLPGATRFSERKGQPSYVEGYKDVDGTPRLAGYVMLSTDITDIPAYSGKPVI
                     TLMGMDTTGHFTGVRILKHSEPILLLGIPEAALVRFNNQYLGKFVGDNIEIGKSRPED
                     NLIGLDAITGATVTVIAQNQVMMTSGTAVAQQVGILKPVIRPAARFTASTATPTWADL
                     VADGSVQRLTVQPEDVGLKGDGRPYMDLWFGYLNQPTLGRALLGADGYASLMSRLKDG
                     EHALFIIKTAGMDSFKGSGFVRGGLYDRVQVRQDKDAFTFRDLDYINLWGLSAAGAPA
                     YDESAIFIIRGKGFSAAYPWKLVFLGNKIDRETGAKTFANFDREYWLDARYLEGGRPL
                     VVKPDPTWLKVWKDKALDIGLFVALLTIIATAYARRDALVRRATHRNKWPVNAFKYTG
                     WVVSIGFVGFHAMAQPSITQVLTWFHALLFQWKWELFLSDPLIFLFWIFIIATTFLWG
                     RGFFCGWLCPFGSLSELLYKLAGAVGLKRLQRHPSPAFHARAKWLKYGLFFGLLAVSF
                     FSMSLAEQLAEVEPFKTTFLVGLLNRSWPYTLFAGGLLGLSIFVERPFCKYLCPLGAA
                     LAMPTTFRWFGLKRKMECTQCKACAKGCGSLAIDPKGNIDARECMLCLDCMVLYYDDH
                     ACPPLAQERKRRGKAGQALTPIGSDGYFIPIHPAPLPRQGETP"
     gene            complement(36055..38001)
                     /gene="nosZ"
                     /locus_tag="PLH85_02065"
     CDS             complement(36055..38001)
                     /gene="nosZ"
                     /locus_tag="PLH85_02065"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HNB63371.1"
                     /translation="MSTEHHPQDDQDLVPGRRKFLNTAALAGLAGAGLSVGLSACKQE
                     AAPTAAPAAPAPAAKAAEGGELPIHPAPGQLDSYYGVWSGGHSGEVRILGLPSGREIK
                     RVPVFNIDCMSGWGITNESKAVIGTRADGSLKYKTGDTHHVHGSYTDGTYDGKYFWVN
                     DKIHGRLARIKGDTFECDKITEIPNIQGFHGIFPDKRDPVDQAINHTTRVFCGNEFHV
                     PQPNDGRDEDDATKYHCLFTCIDAETMEVRWQCRVDGNMDLVASSFDGKLAASNQYNT
                     EGGAHYEDMMSAERDACVFFHIERIEAAIKAGKASTIGDSKVPVVDGTRAANPDPKTA
                     LTCYVPVPKNPHGVNASPDGKYFICSGKLSPTATVIELSQVLAYFDGTLDDLKKAIVA
                     EVELGLGPLHTAFDGRGNGFTTLFLDSQVVKWNIDQAIKFYAGDKNAKYVVDRVDVHY
                     QPGHINASMSETKEADGQFLGVGCKFSKDRFLPVGPLHPENEQLIDIRGEKMKLLADH
                     PVYPEPHDFIIVKRDKIKTRQVYNVDDFPNATKDPKDSGVTRNGNRVTVRLVSQAPAY
                     SLREFKVKKGDEVTLILTNLDKVEDLTHGFAIPKYDINFIVNPQETKSVTFKADKPGV
                     YWCYCTHFCHALHLEMRTRMFVEK"
     gene            complement(38285..41491)
                     /locus_tag="PLH85_02070"
     CDS             complement(38285..41491)
                     /locus_tag="PLH85_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNB63372.1"
                     /translation="MDTRRHRRHRSRLALALAGAFAGFAGLTPSLQAAQFAVGTFAPW
                     PGYAIPNGQWMSADVNGDGKADIIHAVAGADYVNTWLSNGNGSYTVKSFRPWSGYGIP
                     NGVWLTGDINGDGKADIVHAVANTDYVHTWMSNGDGTYTMGTFRPWAGYGIPNGEWRI
                     ADVNGDGKADLIHAVAGADYVNTWLSNGNGTFTVKSFRPWAGYGIPNGVWLTADLNGD
                     GKADIVHAVANTDYVHTWMSRGDGTYTMGTFRPWAGYGIPNGQWMTGDVNGDGKTDLI
                     HAVAGADYVNTWLSNGSGGFTVRSFRPWAGYGIPNGEWRVADLDGDGKADIFHAVANT
                     SYAHQWLSNGDGTYRVQTFSPWAGYAIPNGVWLTGDFNGDRKTDIIHAVANTNYVHTW
                     RSTLPRPGELSLDGLEITQSVQNMAQEVGLVADKRTIARAYVSINGGAAVTVRGVLTA
                     TRTGAAPVSITSLNTVTLDPALAGNLRAKRENLASSLNFEVPPALRSAGATQFRITVQ
                     RVSPGAALVCADCDSSPVGVSFATAAPLRVRVLGLRYSSGTPATVKSPRALDYTLVRS
                     WLGRAYPVSTLTMSTASVNASAAWPFSCNDANAQLATLRANDVAAGTDKRTHYFGLVA
                     DGGGFMRGCAASIPGSADPTAVASGPTGTPSGSFGWDTDGSYGDWYTGHELGHTYGRL
                     HPGSGCGDSADDSHEPFTNGAISGPDGAWTGLDVGDTANGIPMAALPGAQWFDVMTYC
                     RNQWISFYTYNGIRARLTGENALPAGAPAPKALKTSAALPPMAQVGERPEPITGAKAA
                     PVLAAPRRAAPMLAAAPVTGHARPPAIIDGTASPATSTAPASEPPAAAYATPPTPVIG
                     YREVAAAPVANARAVGDDIPMQRGDLLSVVATVNLTRQTGRLVSMSRIPTGLATPVGP
                     EDARIEFLDARGKVLAAYAVPFRRDSDVEAGADQTGLIDAVVPFSTTAAKVRVSAFGT
                     LLGERDIPAKPPRLGKISVLKSKALDEGATLSWTAVAGPLVSYSVQSSEDGGKSWQTL
                     AIGLREPRFQLPPAEGGVTPRYRVIAHDGIHNTIVPVR"
     gene            complement(41776..42759)
                     /locus_tag="PLH85_02075"
     CDS             complement(41776..42759)
                     /locus_tag="PLH85_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNB63373.1"
                     /translation="MAKDKPRIGLDALKALKTAGGARSASVPGRPAAPAPPAQASDTD
                     AALFRREVGRVRPLKAEDYADLSRPRPAPLPRPKTPESTVPDTPRRPSRVDENDPAAV
                     FRAALGDIAPIRDFNRADLSDQRRDTRLGSPPHTDAAPAPVDLNTLHDPAALFRAVAG
                     DVAPLKGSNRADLDRPLPAPHPRQREEDEREVLREALETPLTFEDRLDMGDEAAYLRS
                     GLPRRVLTDLRRGRWVVQGELDLHGLTRDEARAALARFIADALLRGRRCLRVIHGKGH
                     GSPGRIPVLKHLSRGWLAQREDILAFCQARPHDGGDGALLVLLRAGTPVRG"
     gene            complement(42833..43792)
                     /gene="trxB"
                     /locus_tag="PLH85_02080"
     CDS             complement(42833..43792)
                     /gene="trxB"
                     /locus_tag="PLH85_02080"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNB63374.1"
                     /translation="MATRHIKLLILGSGPAGYSAAVYAARANLKPVLITGIAQGGQLM
                     TTTEVDNWPADADGVQGPELMARFEKHALRFNTEMVFDHIHTTYLAEKPMRLVGDAGE
                     YTCDALIIATGATAKYLGLPSEEAFSGKGVSACATCDGFFYKNQEVAVIGGGNTAVEE
                     ALYLANIASKVTLVHRRDKFKAERILIDKVYEKVAAGKIVLELDSTLDEVLGDASGVT
                     GMRVRNVNSGAARDIALKGVFIAIGHKPNTDIFEGQLEMEGGYIVTHGGRNGNATATS
                     VPGVFAAGDVQDHVYRQAITSAGTGCMAALDAERYLDGLGLAG"
     gene            44010..44681
                     /locus_tag="PLH85_02085"
     CDS             44010..44681
                     /locus_tag="PLH85_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB63375.1"
                     /translation="MSVNSTVSTIALRTFPIFQGLGDDRLAGVARCAMMRRVPRGSAV
                     VHEGDRTDFVYFVLTGSLKVMVSDEDGREVILKILGQGEMFGEMGVLDDSPRSASVVA
                     VSPSDLVTISKTDFKRLMQENFELAWHVMCNLAHRLRDADRKIESLALMDVYGRVARL
                     LLEMAEEVDGVKVVRKKISKQDIAKMIGASREMVSRVMKDLGLRGLIEETDGGVILRE
                     KIYDL"
     gene            44821..47112
                     /locus_tag="PLH85_02090"
     CDS             44821..47112
                     /locus_tag="PLH85_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNB63376.1"
                     /translation="MLNRPSTRPVPLPEKIASLLQEARWLLLGVLAAYLAIVLFGYAK
                     SDPGWSHAVYVERVSNPGGRFGAWLSDLLLYLFGLSAWWWVAFLGVGLAVGFRRLQRE
                     HGADRRSLFIVLAGFLVVLVASSGLESMRFHSLRATLPLEPGGLIGVELGSVVGIYLG
                     FTGGTLLLLTLIAAGLSLFSGVSWVEASEAVGGALEAAWNFVLHGAQTWEDRRVGREV
                     AQKREAVVETKRKKEEAAPPPPVRIEPAVVEVPRSERVVKERQAALFVELPGGSLPPL
                     ALLDEPADDVEPPSVESLEFTSRLIERKLADFGVEVKVLAAYPGPVITRFEIEPAVGV
                     KGSQVMNLVKDLARALSVVSIRVVETVPGKTCMALELPNPKRQTVRLSEILGSKAYHD
                     MHSPLTVALGKDIGGQPVVADIAKTPHLLVAGTTGSGKSVGINAMILSLLYKSEPEDV
                     RLILVDPKMLELSIYEGIPHLLAPVVTDMKHAGNALNWCVAEMEKRYKLMSSVGVRNL
                     AGYNKAVAEAVKAGAPLKNPFSITPESPEPLEKLPYVVVIIDELADLMMVVGKKIEEL
                     IARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRMAFQVSSKIDSRTILDQMGA
                     EALLGMGDMLYLAPGTGLPTRVHGAFVADHEVHKVVEFLKSTGAPDYVEGILAPQDEE
                     AGDLLGGGEGGEGSEADPLYDQAVEVVLKTRRPSISLVQRHLRIGYNRAARLIEQMER
                     SGVVSAMSANGNRDVIVPPREGE"
     gene            47109..47759
                     /gene="lolA"
                     /locus_tag="PLH85_02095"
     CDS             47109..47759
                     /gene="lolA"
                     /locus_tag="PLH85_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNB63377.1"
                     /translation="MMIRKMSCAALGLVALLSAGAARADGLAQLKQFMEGTRAAKGTF
                     TQQVFAKSGHKPQQAGGTFAFSRPGKFRWVYDKPYAQVLVGDGRTLWAFDPELNQVTT
                     KKLGQALGSSPAAILAGDNALERSFVLKDAGVADGLEWVEATPKGEDSSFERIRLGLS
                     GGQLRAMLLFDNFGQTTSVQFSQIERNPALDAALFHFVPPKGADVVGDGSPAGAAR"
     gene            47768..49096
                     /locus_tag="PLH85_02100"
     CDS             47768..49096
                     /locus_tag="PLH85_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNB63378.1"
                     /translation="MADLFDALESPRVPLAEAMRPRTIADVVGQAHLLGPGKPLALAF
                     QSGKPHSMILWGPPGVGKTTLARLMAQAFDAEFVALSAVFSGVKDIREAIARAEVERA
                     RGRHTILFVDEVHRFNKAQQDAFLPFVEQGLVTLVGATTENPSFEVNSALLSRAAVYV
                     LQPLTPEELVVLFERARAHACPQLAFEPDARDRLVGFADGDARRLMNLLEQVHTAADT
                     AGQNPVTPAFVDEALSRNLRRFDKGGEAFYDQISALHKSVRGSDPDASLYWLCRMLDG
                     GADPHYLGRRLVRMAVEDIGLADPRALEISLNACATYERLGTPEGELALAEAAVFLAC
                     AAKSNAVYKAYNAARKFVAADASRPVPMHLRNAPTRLMKDLGYGKAYRYAHDEPDAYA
                     AGESYLPDGMARPDWYVPTGRGLEAQIADKLARLRERDRAAQDADRRDEV"
     gene            complement(49093..49362)
                     /locus_tag="PLH85_02105"
     CDS             complement(49093..49362)
                     /locus_tag="PLH85_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63379.1"
                     /translation="MLSIRDCLDYCDLTDEDVAIIAEHHDIPEAAAAQIACGLVQTPE
                     GTLMLTQFLLDLVDRAEERGDRANANRFRAACARFIQAHPVSHTH"
     gene            49624..50910
                     /gene="serS"
                     /locus_tag="PLH85_02110"
     CDS             49624..50910
                     /gene="serS"
                     /locus_tag="PLH85_02110"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNB63380.1"
                     /translation="MLDVQLLRTRLDFVAERLATRGLQLDVAAFQALEDERKTRQTRT
                     QELQARRNALSKQVGQLKGKGEDASAVLAEVASIGDELKANEIALAELLERFNAFVAG
                     LPNLPHESVPPGGSEADNVEVSRWGAIPTFDFEVRDHVDLGARLGGLDFETAAKISGA
                     RFTLMKGGLARLHRALAQFMVDTHTAEHGYTELYVPYLVNAASMFGTGQLPKFEEDLF
                     PVPRADGGKFYLIPTAEVPVTNIVRDELLSADALPLKFVSHTPCFRSEAGSYGKDTRG
                     MIRQHQFDKVELVRIEHPDQAWAALEELTGHAEAILQKLELSYRKIVLCSGDMGFSSA
                     KTYDLEVWLPAQNTYREISSCSCFEGFQARRMQARFKNAQGKNEAVATLNGSGLAVGR
                     TLVAVLENYQQADGSVRIPTVLQPYMGGVTMLAPIN"
     gene            51040..51405
                     /locus_tag="PLH85_02115"
     CDS             51040..51405
                     /locus_tag="PLH85_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB63381.1"
                     /translation="MTSKSFQTITADLSRAVDSLRAEIPGTMQGFSSMARDAMKPGVL
                     SALDKELLATAIGVAARCDGCVGFHVKALIRLGVTREQLMETLAVAVYMGGGPSLMYA
                     AEAVRAYDEFIDLPIRQAA"
     gene            51617..51706
                     /locus_tag="PLH85_02120"
     tRNA            51617..51706
                     /locus_tag="PLH85_02120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51651..51653,aa:Ser,seq:cga)
     gene            complement(51813..53288)
                     /locus_tag="PLH85_02125"
     CDS             complement(51813..53288)
                     /locus_tag="PLH85_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WSC domain-containing protein"
                     /protein_id="HNB63382.1"
                     /translation="MHTRQSLIHLLVLTALAGSPPAWAADIGFDDVPSGTLVDGTYAG
                     SGVLLSSVASQSGHAYASAWQNSNTVTLWNPATGGAFSSAFSADEGAVKAVFAAPQKK
                     VSIDARGVEAIEYLGNDASLQRRPFLEAFDASGKLLAKVYYANWYGDGKAGWGTWQTL
                     TVERPSADIAYVLFSSIANPPGQRGRVNGQGIYGVFDNLQTYPQTQGAYVGCYTDDGV
                     RALPVQLMAAGATVESCMAAAKAHTYAYAGLQYGGTCFAGNAPALRKVADSECAMPCS
                     ADKSESCGGSWRNSVYATGVTPAAPAKPVYQGCYTDDASRALPVTLMANSIANVDTCV
                     AAARARNLAYAGLQYGGQCYGGNSLGKTKVADNECNMPCTAGAGQTCGGTWRNSVYAT
                     GVPSLPKPPANAYKGCYVDTASRTLPIALMSSGATVESCVAAAGREGYAYAGLQWQGQ
                     CYAGNTLPPTKAADAECNTPCIANSTETCGGGWRNSVYATH"
     gene            53802..54890
                     /locus_tag="PLH85_02130"
     CDS             53802..54890
                     /locus_tag="PLH85_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HNB63383.1"
                     /translation="MTETFYDVLRRQGITRRSFMKFCSLTATSLGLGSAFVPKIAHAL
                     ETKPRTPVIWLHGLECTCCSESFIRSAHPLTKDVVLSMLSLDYDDTLMAAAGHQAEAI
                     IEEVKKKYKGNYIVAVEGNPPLNEDGMFCIHGGRPFVEVLKETCADAKAIISWGACAS
                     YGCVQAAKPNPTRATPVHKVITDKPIVRVPGCPPIAEVMTGVVTYMLTFDRIPELDRQ
                     GRPKMFYGQRIHDKCYRRPHFDAGQFVESWDDEAARKGYCLYKMGCKGPTTYNACSSM
                     RWNGGVSWPVQSGHGCIGCSEEGFWDKGSFYDRVTDIKQFGIESNADKVGGAAAGVVG
                     AAVAAHAAVTALARARAKQGEDQNAGGQ"
     gene            54890..56680
                     /locus_tag="PLH85_02135"
     CDS             54890..56680
                     /locus_tag="PLH85_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNB63384.1"
                     /translation="MTVLETQGFKLDNSGKRVVVDPVCRIEGHLRVEVNIDDKNVIRN
                     AVSTGTMWRGLEVILKGRDPRDAWAFTERICGVCTGTHALTSVRAVEDALAIQIPENA
                     NIIRNLMQLNLYVHDHLVHFYHLHALDWVDVVSALKADPKKTSELAQSISSWPLSSPG
                     YFRDVQNRLKKFVESGQLGPFMNGYWGNPAYKLPPEANLMAVTHYLEALDFQKDIVKV
                     HTIFGGKNPHPNWLVGGVPCAINLEGTGAVGAINMERLNMVSSIIDRCIDFIDNVYIP
                     DLKAIASFYKDWTYGGGISSQALLSYGDIPDKANDYTAKSLLLPRGAIIGGDLGKIHE
                     VDLKDPEQVQEYVAHSWYKYADESKGLHPFDGVTEPHFELGKATKGTKTRIENLDEAG
                     KYSWIKAPRWRGNAMEVGPLARYVIGYAQKNPEFKEPVDKLLSDLGLPLQAIFSTLGR
                     TAARGLEASWAAHKSRYFFDKLMANLKAGDLTTAHIDKWDPSRWPAEAKGAGFTEAPR
                     GALGHWIKIKDGRIDNYQAVVPTTWNGGPRDDKGQIGAFEASLMNTPVAKPDEPLEIL
                     RTLHSFDPCLACSTHVMAPDGQEMASVKVR"
     gene            56813..57517
                     /gene="cybH"
                     /locus_tag="PLH85_02140"
     CDS             56813..57517
                     /gene="cybH"
                     /locus_tag="PLH85_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HNB63385.1"
                     /translation="MLSQQDMLEAERHGRQVRSIYVYEAPVRLWHWINALAIVVLAVT
                     GYFIGKPLPTMPGEASDNFLLGYIRFAHFAAAYVFAVGLAGRVYWAFVGNHHARQIFK
                     LPLTNAQWWSEVFFELRWYLFLEKHPKKYVGHNPMAQLMMFFFFTVLAFGMLFTGFAL
                     YSEGLGLGSWADRLFGWVIPALGGSMMTHSLHRLGMWIMMSFVVVHVYAAIREDIMSR
                     QSLISTMVSGWRMFKD"
     gene            57795..58406
                     /locus_tag="PLH85_02145"
     CDS             57795..58406
                     /locus_tag="PLH85_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HNB63386.1"
                     /translation="MDTSPRVLVLGIGNLLWADEGFGVRCVEALAERYSVPPQVTLMD
                     GGTQGLYLLPYVEEADCLLVFDAVDYGDPPGTLRIVEGAEVPRFMGAKKMSLHQTGFQ
                     EVLASAELLGRLPRELVLIGCQPVELEDFGGSLRDAVKAQIGPALDVAAEFLRGWGAA
                     PLPRADDAPRLNAGSLGMAAYEAGRPSEQEACRFGDARFLAGD"
     gene            58409..58717
                     /gene="hypC"
                     /locus_tag="PLH85_02150"
     CDS             58409..58717
                     /gene="hypC"
                     /locus_tag="PLH85_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNB63387.1"
                     /translation="MCMGIPCRVVDSQGWTALCDGPAGPVSIDLALVGPQAPGTWLLT
                     FLGAAREVISAERAAQVGDALAALEAALAGETDRIDALFGDLVGREPTLPEHLRKELP
                     "
     gene            58714..59154
                     /locus_tag="PLH85_02155"
     CDS             58714..59154
                     /locus_tag="PLH85_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63388.1"
                     /translation="MTVHFAPAPSATERLEAALRRLTERHAFTRVDAAFEPPAGTAVL
                     LLSDDPQRSPEALDVIVVLPEALRALGRPLSACVADAEASAVLARRFGVARLPAVVAF
                     RDGAWLGAFSGILDWAPFVTELARLLDAPPGRPPIFVASAEARP"
     gene            59151..59999
                     /locus_tag="PLH85_02160"
     CDS             59151..59999
                     /locus_tag="PLH85_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HNB63389.1"
                     /translation="MKPFPIPVVPVGPGSQTDEAPDYLAMPGGMATFQAPRLPEGAQA
                     ADVAHAASLLAGLVDAMAGAAFDGPPLVLPLDAETPGVREVLTQSLGFGEVSAFTVAP
                     TRLRVQETAFASLWRVLEEGEDGRLLHDRLTVCPVPPELYAAMRATARPDLVPPAVLP
                     GMMNGEALLAEVRQQAAAHRARRPAHVINLTLLPVSEADLDYLYGALGHREVSILSRG
                     YGNCRVTSTRLAHVWWVQYFNSMDTLILNTLEVVDMPDVVLAAEEDFRDSIERLREYV
                     ALLAEA"
     gene            60003..60212
                     /locus_tag="PLH85_02165"
     CDS             60003..60212
                     /locus_tag="PLH85_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNB63390.1"
                     /translation="MFEGSFLGDAARLADTDRLECGICWTVYDPAEGDPHWGIPPGTP
                     FAQLPEHWRCPVCDAPQHKFMVLRD"
     gene            60205..60765
                     /gene="hybE"
                     /locus_tag="PLH85_02170"
     CDS             60205..60765
                     /gene="hybE"
                     /locus_tag="PLH85_02170"
                     /inference="COORDINATES: protein motif:HMM:NF023365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HNB63391.1"
                     /translation="MTEAPRPATVPVAAAELLARIEAHYRRVAATAMADVPLCNPRLA
                     VRGVGIEDWDGQRIVALVTPWAINLLRLPGSAPLPSRGPLAKHVWRLPSGDYAFVEAG
                     DVGFGPFQSCSLFSPVLEFDSQAAACETALAALLALLRPPLAVPEAVAVADARPSGTP
                     APSRGVPPPAPAEVPSRRRWLLGRGA"
     gene            60762..61604
                     /locus_tag="PLH85_02175"
     CDS             60762..61604
                     /locus_tag="PLH85_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63392.1"
                     /translation="MSTLSLAGTLKVTARWAFGGLHDLQVDLKRPPVARALVGLTPGE
                     ALARLPVYFNLCSQAQTEAGRLALAAAGMPLGTPVESWRLWAECLHEHLWRLLLDWPR
                     VMGAVPETAAFAAWRACRAQGREALADATRELLEHRVLAGPLGGLLARLEVRLHVGQG
                     DDPVGAGLEARVQAIRDACAALAANRDYPRGSLGGEAGGRAEGWVHTARGALHHRLRL
                     AGGRVADWEIDAPTDRNFADAIALRRRLPEHLPDPREAKAALERAVLLLDPCVPYGVE
                     IDHA"
     gene            61597..61938
                     /gene="hypA"
                     /locus_tag="PLH85_02180"
     CDS             61597..61938
                     /gene="hypA"
                     /locus_tag="PLH85_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNB63393.1"
                     /translation="MHEMSLAEGIRQIVEDAGRSQGFGRVRAVVLEIGQLAAVEVDSL
                     RFCFDVVMKDSIAAGATLTIETLPGTGWCMQCARSVPVTALYEACPCCGSYQVQPTGG
                     TEMRVKELEID"
     gene            61944..62891
                     /gene="hypB"
                     /locus_tag="PLH85_02185"
     CDS             61944..62891
                     /gene="hypB"
                     /locus_tag="PLH85_02185"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNB63394.1"
                     /translation="MCTTCGCGAGETRIEGDALHEHVLADGTVLRHSHAADHPWRPRK
                     VRGVPPEASPSAADDPAVATDDGALHYGHGPAGAHAPGLSQGQMVRIEQDILGKNDGL
                     AAGNRAWLAARGILALNLVSSPGSGKTTLLVRTIEALQRRVPVCVVEGDQQTSFDAER
                     IRATGAPALQINTGKGCHLDAHMVGRALERLAPPADSVLLIENVGNLVCPAAFDLGEA
                     AKVVVLSVTEGEDKPLKYPDMFAAARLMLLNKTDLLPYLNFRVDDAIAYARRVNPALE
                     VIQLSATSGAGFDAWLAWIDARRAEASVRVRDVAAGGEG"
     gene            62896..65259
                     /gene="hypF"
                     /locus_tag="PLH85_02190"
     CDS             62896..65259
                     /gene="hypF"
                     /locus_tag="PLH85_02190"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNB63395.1"
                     /translation="MLRKIDPERVARRIVVRGVVQGVGFRPFVFRIARRHGLDGWVRN
                     DGTGVEIRVQGAAVRVERFIHALAEQAPPTARVSLIEPEVIAPDPHRSGFSILPSAGG
                     QIATTIGPDLAVCGDCLEELFDPQNRRYRYPFINCTHCGPRYTLTRRLPYDRATTSMA
                     GFALCPECGSEYADPANRRFHAEPNACPACGPRLSLREVHDVPALVRDPISATGQRLR
                     LGEIVAVKGLGGFHLACDAANPFAVATLRDRKRRPAQPFALMVANVASARRLARVSDE
                     EARLLESPERPVVLLAKAADFDRQFTGIAPGLDEVGLMLPATPIQFLLFHEAAGRPDG
                     TAWLQAAQDLTLVMTSANPHGEPLVRENEEAFERLEPVADEVLFHDREIVARCDDSVL
                     RVRADLPGHVQFLRRARGYTPLAWPLGRSGPAVLAFGAHLKATVCLTRGAEAFLSPHV
                     GGLENPAACAVLDETAERLQALLATVPDVVAHDLHPDYHSTRAALALAERLGVPAVAV
                     QHHHAHIAAVCAEHGIDGPVLGFAADGSGLGSDGLPWGGELLRVDGADFTRLGHLAPL
                     ALPGGDKAAREPWRVACGVLHALGLDELAARRFADQDVTRLLDLLARGLRCPPTTSLG
                     RWFDAAAGLLGVRSLSLYEGHAAMALETQARRYGAALPHPGGWHMEERPGDGSVLSLY
                     PLFPQLADETAPARGAAVFHATLVDGLVRWIGDAARAQGLRRVALGGGCLLNRLLAEG
                     LRKGLLARGLEVFEAGEFPTGDGGIALGQAWVARLAVRDGRIGCGPR"
     gene            65280..65516
                     /locus_tag="PLH85_02195"
     CDS             65280..65516
                     /locus_tag="PLH85_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNB63396.1"
                     /translation="MCLAIPTRVVELLPDAQALVDLGGVRKTVSLELVDDVAPGDYVI
                     VHVGYALTRLDADEAERTLALMAEAGLDVTGGGA"
     gene            65513..>66224
                     /gene="hypD"
                     /locus_tag="PLH85_02200"
     CDS             65513..>66224
                     /gene="hypD"
                     /locus_tag="PLH85_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNB63397.1"
                     /translation="MKYVDEFRDGAVARGLAAAIARAVQPDRRYAFMEFCGGHTHAIS
                     RYGVADLLPPAVRMVHGPGCPVCVLPIGRIDQAIRLALDADVTLCTYADTLRVPASDG
                     LSLMRARARGADIRMIYSAADALALAQREPQRQVVFFAIGFETTTPPTALVVRQAAAL
                     GLTNFSVLCCHVLTPAAITAILESPEVRTLHTVALDGFIGPAHVSTVIGSRPYAQFAA
                     DYRKPVVIAGFEPLDVMQA"
BASE COUNT         9858 a        21101 c        24240 g        10925 t
ORIGIN      
        1 acagactgtg agcgctcgtc gacaaggtct gagcacgggt catcatgatc tttgcgccca
       61 tgaacaggct ttgagcgccg gtgcacatcg tctgtcgatg gggccgacaa ggatgcgcgg
      121 ggggcagatg attcagggca cggcccggct tgagggggcc gcaagccgtg atggagaggg
      181 ccggccgacc cgacctggta ccgtggcccc gatccggttc ggacggtgct gaatcgtatg
      241 ttgccccgtc cgaattcgat tcggaccgct ccgattgata catggcgccc tccgaattcg
      301 atgtggacgc ctccgaatcg aattcggacc cggctgaatg cgatgtggac ggctccgatt
      361 cgtatgtgga cggctctgaa tcgaatatgg accgctccga tttcaattcg gagcggtcca
      421 catcacattc tgcctgctgc gattccttga gcgggggggc gtgtcgatct ggggccggcc
      481 gccggatcga ggccgcgggc ggcgaggccc gcggggaaaa ctgctgatgt caggtgccgc
      541 gcgtcaatcc cgtccgcggc catggccgtg accgtggccg ccgccgtgat cgtcccggcc
      601 gccgccgcgg cggtcgtcgt cgcgccagcc gtgatcgcgg tgatcgtggt caggatgtgg
      661 gtcatatccc cgatcacgcg gttcgggata gccctggcgt tcgcgatacg ccgggacgta
      721 cacgttgttg taccaacggt ccgtcacgaa atacaccggc cggccgcagg cgccgtaatt
      781 gcgacagtat cgcgaccagt ttttctggtg gccgggcggt acccgcagat agaccggttc
      841 catgtatccg gggccgcttt cgatgatgat ggggcgggca ttgatcagtg ccgggcgggg
      901 gaagtcgccg atattcagtt gcccgtagaa gcccggctgt ccgacgctga tcgagacccc
      961 gatgtcggcg gcctgggcgt tgatgacttc aaaagaaagc atcgctagca cgcacgcgaa
     1021 tttgaccttc aagatgttcc cctttcagtg gtggcgtctg gctcctcgcg gttcaaggag
     1081 gccaggcggg tctgttcctc aaaaaatcat cgaaacccct tcaatgacgg ctgagctgcc
     1141 gtggacctga gccggggcga tcgaggcggt gtccgatttg agagggagtt tgagtcggga
     1201 cgcggcgggc aagttcaggc gctcccttcc cgtgaagggg gccgcagggg cgtcatcccc
     1261 gggagggtgg gccggacgcc gtgtgccggc gcccgtccgt aacgctcaat agatctcgcg
     1321 tgtttccagg aagcggatgt tcggaaagcg ttcctgggcc atctgcaggt tgacccggtt
     1381 gggggcgagg tagacgtagt cgccgttgcc gtccagggcc atgttggcgg gggatttgtc
     1441 ggcgagggcc ttgaggtcgg cgtcgctgcc gcgcagccag cgggcggtgg cgtaggcgca
     1501 ggattcgaac atgacgtcca cgccgtattc gaattcgagg cggtgggcga ccacgtcgaa
     1561 ctgcagggtg ccgacggcgc cgaggatgag ttcgttggtg gtggtggggc ggaagagctg
     1621 ggtggcgcct tcctgggcga gctgttccag gcctttctgg agctgcttca tcttgagggg
     1681 gttcttgatc tgggcgcggc ggaagtgttc cggggcgaag ctggggatgc cggtgaattg
     1741 caggtcttcg ccttcggtga aggtgtcgcc caggtggatg gtgccgtggt tggggatgcc
     1801 gatgatgtcg ccggcccagg cttcgtcggt ggtgttgcgg tcctgggcca tgaaggtgat
     1861 ggcgttgttg atggcgaggg acttgccggt ggacacctgc ttgagcttgc cgccgcgctc
     1921 gaagcgcccg gcgcacacgc ggatgaaggc gatgcggtca cggtgcttgg ggtccatgtt
     1981 ggcctggatc ttgaagacga agccggagaa cttgtcttcg tcgggctgca cctggcgggt
     2041 cttggcgggg cgggcgaggg gcgcggggga gagatccacc acggcatcca ggaggctctg
     2101 cacgccgaag ttgttgaccg ccgagccgaa gaacacgggg cactgcttgc cgcccaggta
     2161 ggcgtcccgg tcgaagctgt gggaggcgcc gcggacgagc tcgatgtcga ggcgcagctc
     2221 gtcggcctgg tcgccgatga gggcgtcgag gcgcgggttg tcgaggcctt cgatgatctc
     2281 ggaggtgccc ttttcggcct gggggtcgaa gaactggacg gtgtcgttgt agaggtgata
     2341 gacgccgcgg aagcgtttgc ccatgccgat gggccaggtc atgggggcgc actggatgcc
     2401 cagcacggat tcgatctcgt ccagcagctc gatgggctcg cggccttccc ggtcgagctt
     2461 gttgatgaag gtgacgatgg gggtgtcgcg caggcggcag acgttgagga gcttgatggt
     2521 ctgggcttcg acgccgttca ccgagtcgat gaccatgacc gccgagtcca ccgcggtgag
     2581 ggtgcggtag gtgtcttccg agaagtcctc gtggcccggg gtgtcgagca ggttgatgac
     2641 gcagtcgcgg tagggaaact gcatcaccga cgaggtgacg gagatgccgc gctgcttttc
     2701 cagctccatc cagtcggagg tggcgtggcg cgcggccttg cgggcgcgga tctcgccggc
     2761 cacctggatg gcgccgccga accacaggag cttttcggtg agggtggtct tgcccgcgtc
     2821 ggggtgggaa atgatcgcga aggtgcggcg gcgggcaatt tcgtgggcgg cggacatggt
     2881 gcggtgcggg aaaggcagaa aagtccgcga ttatacaagc ccttgcgccg cgggccccgt
     2941 gtcttctccg cgcttcgggc gcatgcgtgc tgcggcgtgt tcagaactcc agcagcatca
     3001 ggcgccggcc ggcgctttcc agcctgccgg cggcgcgaaa gccgaagcgg gcgaagcgcc
     3061 gcgggcgtgg ggtggagacg agcacgcggc cgtgctcgaa ggtctccatc acccaggcga
     3121 cgcgggtcat taccagggcc cgcgcgatgc cctggccacg ggcgtgttcg gcgaccgccg
     3181 agaggtagag caggtgggtg tcgctggccc agtcggcggc cttgatgccg cccacgccga
     3241 gcacttcatc gtcgcgccgg gcgatgaaga agcgccggta atagggcagc ggggcgctgt
     3301 cgagctggga ctggatgcgc gccgggccgt agcgttccgg gtcgtcgaag ctggccagca
     3361 ggatggcggc ggcggcctca cgcagggggc cggggccgtc gtcgaggggg gtgatgacga
     3421 ccccgtcggg caggtcggag gcaaggcggg cgcgggcttt catccggcca ttggaaccgc
     3481 accatgttgc agccgtgtgg ctgtggcggg caggccggat ggagcgcccg gcggggcgcg
     3541 tccggcaaaa agatgtgtcg ccgacggctc tgcctaaagt tatagcggac ggcggccgtt
     3601 ggtgacgcca gagcggcctc cccgtatggg tcgccaccga caggaaaatg tcaccgcctg
     3661 cttcagcgac tgccgatcca cattttggac gtactgccat gaccatcgtt aaacgcctgc
     3721 tcctgctcgt gggagccgcc ctcatcagtc tgctcgcann nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnngc acgggattcg gccacctggt ttgcggtggg catcctggtg
     3901 gtggcctgtg ctgcgatggg ggtgatcagc ctgatgacga tccgcgccat cgccggccgg
     3961 ctcgaggagg ccaacgccct ggcagcccgt gtggcggccg gcgacctgac aggcggcaag
     4021 gcgtcaccga acagcggttc ggacgagatc ggccagctgc tggcctccct ggacgcgatg
     4081 cgagtggatc tggcccagac gatttcgggc atcgccacga atgcggagaa cgtggtgggc
     4141 tacgcggccc agctgtcgtc ggcggcccag caggtgtcgg tgagcaccga acagcagtcg
     4201 cgcaacacgg cggcggcggc ggcggcggtg gaagaactga cggtgagcat cgaccacgtg
     4261 ggttccagcg ccggcgatgc ccgcgtgcgg gcggccgatg cgggcagcaa ggcggtggac
     4321 agtgcccggg gcgtggatac cgcggccgac aaggtggggc aggtggcgaa ccaggtggag
     4381 accacctcgc agcagatcca gaccctgtcc cagcaggtgc agcagatcga caagatcacg
     4441 acggtgatcc gtgaagtggc ggatcagacc aatctgctgg cgctcaatgc ggccatcgag
     4501 gcggcccggg ccggcgagca gggccgcggc tttgcggtgg tggccgacga ggtgcgcaag
     4561 ctggcggaac gcaccaccag ttcggtgcag gaaatcagca acgtggtggg cgcgatccag
     4621 cagggcgcgg tgggcgcggt gaacagcatg caggtgagcc gcgatctggt ggcggcggtg
     4681 gtgggctcgg cgcggacggc gagcgaatcc atgagcgaga tccgcgaatc cgcgtccacc
     4741 atgagcggcg tgatcgagag catctccgat gccttgcagg aacaacgggc ggcgtccacc
     4801 gagctggccc gcaacgtgga atccatcgcc cagatgtccg aggagaatgc ggcggccgtg
     4861 gggtcggtgg ccgagacggc ccatcgtctg tcgtcggtgt cggacgccct caagtcaaac
     4921 gtggcgcgct tccgcgtctg agtgcatcgc ccgtccgccg cggccgcccg aagcgatgag
     4981 cttccggcgg ccgcggtgtt ttgaggaggc gcttgcgcgg ccggggccgg gcgggtcaga
     5041 aggggcgggc gagggccgcg gactgcttcg ggtcgcggaa caccagcggc tgggcagtcg
     5101 gctgctcggc ggcctgcagg gcggtctgga tgagggcgtg gtccggcgac ttgggacaca
     5161 ccgggtcggt ggcgtccggg ttgccggtga gcatgaaggc ctggcagcgg cagccgccgt
     5221 ggtcggcttc cttgtcgggg cagctccggc acgggtcggg cagccaggcg gtgccccggt
     5281 agcggttgaa gaggggtgag tcgtgccaga tccacgccag gtcgtgggcg cggacgtcgg
     5341 ggaaatccag tccgggcagc atgcgcgcgg tgtggcaggg cagggcgagg ccgtccggcg
     5401 tgatggcaag gaagacttcg ccccagccgt tcatgcactt cttgggggcc gtggaggcgt
     5461 agtccggcgt gacgaagagg atgcgcatgg ccttgccgag gcggacgcgc cagcgttcgg
     5521 tgacccgctc ggcttcggcg agctgggcgg cggtcggcat gagggcggcg cggttgaggt
     5581 aggcccagcc gtggtactgg gtgttggcca gctccaggta ttcggcgccg atctccgcgg
     5641 ccatctcgat gatgcggtcc aggtgctcga tgttgccccg gtggagcacg cagttgatca
     5701 ccgtggggta gccggcggcc ttggtgaggg ccgcgacgcg gcgcttcaga tcaaaggtgc
     5761 gggtgttcgt gatgaggtcg ttggcctcgc gggtggcgtc ctggaaggaa agctggatgt
     5821 ggtccagacc ggcgtccttg agggccgcga ggcgcgcttc ggtgaggccc acgccggagg
     5881 tgatgaggtt ggtgtagaag cccaggcggc gggcttcggc gacgaggatc tcgatgtcgt
     5941 cccgcagcag gggctcgccg ccggagaggc cgaactgggc ggcgccgagg gcgcgggcct
     6001 ggcggatcac gtgcagccag tcgtcggtgg agagctccgg cccggcctgg gcgaaatcca
     6061 ccgggttgta gcagaagggg cagtgcagcg ggcagcggta ggtgacctcg gcgagcagcc
     6121 acagggggcc ggggcgcggc gggatgagga gcgcttccat cgcggggctt tcaggtcggg
     6181 gtccagtcga tccagctgcg ctcttcggcg aggcgcagga aggcctccac gtcggcggtg
     6241 aggctgctgg cctggaaagc ggcctccagc tcggcgacga tctcggcgac gctgcgggtg
     6301 ccatcgcagc gggcgaggat ctcgccggcg ctgcggttga gcttgaccat gccttccggg
     6361 tagagcagga cgtgggcctg ctgggcgggt tcccactgga agcggaacag gcggccgatg
     6421 cgcggatggc tggatggggc gggcgtggaa tgcatggcgg gggctttccg tgggttcagg
     6481 ccgggcggcc gccggcgagg ccgtagtgct gggccatggc gtcgttcatg gtccacagga
     6541 tgtcgagctt gaacttgagg atgtccaggg cgcgttgctg ggcggcgcgg gtgtcgaagt
     6601 aggcgagggt gatggcgagg ccctgttcca cgtcgcggcg ggcctgggtg acccggttgc
     6661 ggaagtactg cagcccgtcg gattcgatcc acgggtaatg ctcgggccag ttggccaggc
     6721 gctgcttgtg gatggtgggg gcgaagagct cggtgaggga ggcgcacacg gcttcctgcc
     6781 agggtcgctg gcggcagaag ttgaggtagg cgtcgcaggc gaagcgcacg gcggggagga
     6841 cgtggcgcag gtcggtgatc tcgtcccggg cgaggccgac ggcctggccc aggcgcagcc
     6901 aggcctcgat accgccggcg tcgtcgccgt ggccgtcgtg gtcgtggatg cgctggatcc
     6961 attcgcggcg gaccccccgg tccgggcagt tggcgaggat cgcggcgtcc ttctggggaa
     7021 tgatgatctg gtagtagaag cggttggcca cccagccacg gatctgctcc gggcgcgctc
     7081 cacccgtgtt gagcaggacg ttgaaggggt ggtggatgtg gtaggccgcg cccttgtcgc
     7141 gcagctgcgc ttcgaattcg gcaggcgtcc agggggcggg cttcacgggg gtgttcacag
     7201 ggcgatctcc atgccgtcgc aggccacttc gatgccggcg gcggtgaggg tggcccgctc
     7261 cggcgagtct tcgtcgagga tcgggttggt gttgttgatg tggatgagga cgcgccggac
     7321 gtggcgcggc aggcgggcga gctgttcgat catgccgccg gggccgctct gggccaggtg
     7381 gccgatgtcg cgggcgcgct tggtcgacag gcccaggcgg atcatctcgt cgtcggtcca
     7441 gaaggtgccg tccaccagca cgcagtcggc gccggccatg gcgtcgaaga cggcgtcgtc
     7501 cacctcggcc aggcccggtg cgtagaacac ccggcggccg ctggcggggt cgcggatggt
     7561 gaggccgatg ttgtcgccgg gcacaggctg gccgcggtgg ggcgagtagg gcggcgcggc
     7621 ggagagcagg ggcatcgcgc gccactccag tgcctcggcg ccctggggcc ggaacacgcg
     7681 gccgtcgagg gggatggcgc ggcggtcgat gccgcagtag tggtcgagca ggtgcaggat
     7741 ggggttgccg cgggtgaggt cttcatagac cgggtcggtg gtccacaccg gccacgggct
     7801 gcccttctcg cgcagcatgt agaggccggt ggtgtggtcg atctgggcgt ccaccagcac
     7861 gatggattcc acgccgcagc cgcgcaggcc gtcggtgggc caggtgccgg caaaggcctg
     7921 gaactgctgg aggatgtccg gtgaggcatt gaccagggtc cagcgggcgc cgtcggcgct
     7981 cacggcgatg gacgactggg tgcggcggcg ggccgacagg ctgccgtcgc gcaggccgcg
     8041 gcaattgcgg cagttgcagt tccactgggg aaatccgccg ccggcagcgg cgccgagcac
     8101 atgaacgcgc atgggtcgag attggcgtgg gttggtgaag gcgggacggc cgcccagggg
     8161 ggctgaagcg gccgatcgcg gcgccagggc gttacttggc ggcgatgtac atcgtgattt
     8221 cgaagccgaa acgcaggtcg caggcgtgcg gggtggtcca ggtcatgacg tgtctcctct
     8281 tgaggggtgg atgaaacggc cggcgcttgc cgatccgtgg agcgtagtgt ggggacggcc
     8341 cgcgcggccc gcctcacgaa aatcatgaat ttgcggcggt gggctggcgg tgtccggcga
     8401 gggtggcgac actggcaggg tgcggccggt gcgccgcggt tccgtcgatc cctctccatg
     8461 tccccgtcac ccgcctgtgc cgtgcctgcg gcgttttatc tggacgtctc cgacggccat
     8521 cgtctcttcg tggcgactgc cggtgcgccc ggcggcgagc cggtgctggt gctccacggc
     8581 ggccccggca gcggctgttc gccgcgcctg gcggagctct acgatcccgc ccgccactgg
     8641 ctggtgatgc ccgaccagcg cggcgccggc cgctccgcgc cggcaggcgc ctgccatgcc
     8701 aacacgaccg cccaccttat cgccgacctg gagcgcctgc gccgccacct gggcatcgcc
     8761 cgctggcggg tgacgggcgg atcctggggc gcgaccctcg cgctgctcta cgccgcggcc
     8821 catcccgcgg cggtcagcgg tgtcgttgcg cgcagcgtct tcctgccggg tgccggcaac
     8881 gtggcggctt tcttcgccgc cgggggcgac gttccggccg agcgggtcgc cctggccgac
     8941 gcggccggct gcccggccgg tggcgatgtg ctggccgcgc tggcccgacg tctggacgat
     9001 cccgagccgg cccgcgccgt gcgcgcggcc gtggcctggg cggcctacga acgcgccctg
     9061 gcggaacccg aggcgccgcc gcaggccgac ctggacgccg ctgcggcgga gtgcctggtg
     9121 cgcaagtacc gggtgcaggc ccactacctg caagcgggcg ccttcgtcac cgaggccggc
     9181 tttctggatg cctgtgcgag cctgcccgga gtgccggtga ccctgatcca cggccgcctc
     9241 gaccgggtct gcccgccgga caacgcccgt cgcgtgcacg cccgcattcc cggcagtcgg
     9301 ctggtgtggg tggatggctg cgggcacgcg gccttccatc cggggatggt ggcggcgtgg
     9361 cggcaggcgc tggggggcgc gggctgaggg tgctcgctaa aggcgtgccg tgtcgcgggg
     9421 acggtcgtgc aagctgagcc gccttgcggc agcgtcggga gggcgtgtcg gccagcgctc
     9481 cgtcagaccc gcgccacggg cgcatacggc gcgctgcggc ggaaccccgc gccgcgcgcg
     9541 gcggggtatt tcgaaggatt gcgccgcgct gccgggcccc ggcaccttcc acggcgtggc
     9601 gcggtcgtta aaatagtgaa cgacgtacaa aaataaacat ggtaatttat tgaccttcgt
     9661 atcaatttca agccttgccc tccgcctgcg gccgggcatc gatcactgag gtctgtcatc
     9721 catgtccacg cccccccgtt ccgatccagg ccgccgtgag cgcaagcggc tcctgcagct
     9781 cgaccatctc gccgacacgg cctgggcgct gtttgacgcg gaaggttatg aggccgtgac
     9841 gatggagcgc atcgcggcgg tggcggacgt ggccaagggc acgctgtaca agcacttccc
     9901 ggtcaaggag gccttgctgc gtcatcgttt ccaccgggaa ctgcgtgagg ggctggagcg
     9961 cctgcagccg gtggtgctcg ccgaggcgcc gggggcggcc cgattgaggc gtttcttcga
    10021 tctgagtgcg cagtggagcg agcggcatcg ggaaacgctc ctgccctatg tgcgttttcg
    10081 tcttgcggag ccctgccacg gggacgattc gcagcgcagc gggctggcgc aggtgtttac
    10141 cgggctgatc gcggacggtc agggcgccgg cggatttcgc accgacatcg gggcggaggt
    10201 gctggcgaat tacctggaat tcctctatct ggcgtcgctg ttgcgctggc tgaccggcgc
    10261 caccccggtg ctgcaggcgg agttcgagcg gatgctggcg gtcttcctgg gcgggctgga
    10321 ggtgcgttca tgaacggcgc cttgctgaat cccgaggagg tgcgggtgac cgacgccacg
    10381 gtgaccggcg ggaagggccg gcaactggcc caactgcgcc acttcggtct gccggtgccg
    10441 gatttcgcgg tgctgcccgc agcctggagc gagggccgcg gcgaggcctt ccctgctgcg
    10501 ctggtgacgg ccctgcacga cggtctggcg gcgcgcggct ggctggagca gcccctggcg
    10561 gtgcgttcgt cggccgtcgg cgaggactcc gcggcggcgt ccttcgccgg gatctacaca
    10621 tcctgcctga acgtgcaggg gctcgacggg gtggtcgcgg cggtgcagac ggtgtgggcg
    10681 tccctggaca gcgacaccgc gcgggcctat cgcgcgcgca tggggcacgc ggggcccgcg
    10741 cgcatgggag tgatcgtgat gcccctgctg gcggccgagg cgtcgggcat cggctttacc
    10801 tgcgatccac tgacggggcg cgaggaccgc ctcgtgatcc acgccaaccg gggcctgggc
    10861 gagaccctgg tggggggcga gacggccggc gacgagtatg tgtttgccga ggacgtggag
    10921 gacgcctggc ggctgatcga gtgccggccg ggtcccaaga cctgcatgag cgtaccggcc
    10981 ccaggaggcg gcacgcggcg cgtggcgctg ccggatgcgg cggcggcgcc cctctttgac
    11041 acggagcggg ccgaggcgct ggcggcgctg ctgcgggacg cggcggtggc gatggatttc
    11101 gtggcgccct tctacgatct ggaatgggtg tgggacgggg cccgcttctg gctcacccag
    11161 gcccgcccgg tgacgcgccg gccacgtcac gcgtatggcc cgctgcaggc ccagccggcc
    11221 atctggaccc gcggcaacac ctgcgaggtg atgcccgagc cgctccagcc ggcggactgg
    11281 aacttctccc ggcggggtgt gaacgatctg ctcgtgcagg gttacgtgct ggccggcttt
    11341 cctgtgctgg cgggggccca gcgggcgggg ctcttcgagg ggcggctgta cctggaggcg
    11401 tcgctgctcc agtgggaagc ctgggacgcc atcgggatga agccggagcg cctgaacgcg
    11461 ttgatgggcg gccatcagcc ggccatcgac gtgacgccgc ccggcgggcg cgagcgcctg
    11521 cgccgcgccc tcacgaccct gcgctacctg gtgcgggcgc cggccctgcg gcgccgcggc
    11581 gaggcggaga tcgcagccat gcatgccctt gcgcggcagg tccgtcaggg accgccgccg
    11641 cgggaccggg acgccctgcg gcggctgctg tacaccgtgt cgcggcccgg gcggacgtcc
    11701 atcggcgcct tcttcctgca aggatccggc ggtgccagcc tgtccttcct ggtggagcgc
    11761 ctggaacact tcttccccgg cgagggcgag gccctggggg cggccctgct gaccgggggg
    11821 acgcccagcg tcacggcgca gcagggatac gatctgctcc gcctggcgcg tctggcccgg
    11881 gcggccggct tccccgcaca ggtgccggcg cagggcgact atgcgcgggc cttcgccgag
    11941 ttcatggagg tccacgggca ccgcgggcac tacgagacct acatgcacag cgcgtcctgg
    12001 cgggagcggc cggagcgtct gctcgaacag ctgccggccc tcgccgaggt ggacgaggcg
    12061 gccctgcgcg cgcgccagca ggacgcggcg gcggcagcct ggcagcgcgt ccggcgggag
    12121 gtcccgttct ggcatcggcc cctgctccgc tccctggtga aggcggcccg ccgggaatgc
    12181 aaccagcggg aggcggcccg cagcggcctg atcgccctgc tggaggtgtg tcgaatggcg
    12241 tggcgcctcg cggcggacct gctgcgggcg gacggggcgc tggcagagcg ggacgatgtg
    12301 ttcttcgtgc tgcccagcga ggcggagcgg gccgtggatg gccttgttcc accggccggc
    12361 ctgcgcgccc gggtggcggc acgcaaggcg ctttacgaag cgtggcaggc ggtgacgccg
    12421 cccgaatacc tgtgcatcgc cccggccggt gaatggctgg cggccccggc ctggcccgcg
    12481 ccgccgcacc ggccgggcga aggctggcgg ggcgtggcca ccggcaccgg gcgggcccgc
    12541 gggcgggcgc gcatcctgcg ccacccgtca gagggcagtc gcctgcaggc cggcgagatc
    12601 ctcgtcgcgc catccaccga tccgggctgg acgcccctgt tcctcaaggc cgccgggctg
    12661 gtggtggaga ccggcggcta catgtcccac ggcgccatcg tcgcgcggga gtttgccctg
    12721 ccggcggtgg tcaatctgcc gggcatcctg gacgggctgc aggacgggga tgaggtggag
    12781 gtggacgggc gcagtggcga ggtgcgcctg atccgcccct gagcaggggg aggcggtagc
    12841 cggttcccgc cgggaacacg cccggcgtca gcggtcgagg atgcagcgca tctccggggc
    12901 gccatggccc cacaccaccg tggcctcgcc ctggtgttcc cagaagcttt cattgcggcc
    12961 cacgtagcgg gtgccgctgg cggcgggctg caggaacatc agggacgtct ggtctccgcg
    13021 ctcggcgatg agggtgggcg gatcggtctg gaaatgggtg atgagcactt cgtccgcggg
    13081 ctggccgtta cagctgaaac ggaaggggcc gcgatgggca acgagccggt agcgggcctg
    13141 cagttcggcg atccgggtga cgtaggcatc ggccacgcag gcgcgccggt cgtcggactt
    13201 ccagcaatcg tcgcgtcccc ggacccagcc gcgctgctcg gccttgagca cgggcggacg
    13261 ttcgtgcgct gcggtgcggg cggcctgggc atacacccgc tgcaggtcgc ggtcgagggt
    13321 gcccagggct gtgtcggcgc agaccagatc cggaatgctg cccggctgca cgccgcggca
    13381 ggaaaaggtc gggccggggc gggaggcgta caggcgggcc tgctcgtccc gctggcgcag
    13441 ggccgcttcg atgcgttcgc accaggcgcg gctgccgcgg gcgggcaggt cgtcccggcc
    13501 gcgcaggccg aggcgccatt ccaccgcgga ggcccattcc gccgagccgc ggtccgggcc
    13561 gtggccgctg cggtcaaggg gctggctctg gcgttcgatg gctgcgagac gcgccggcga
    13621 acagggggcc gcgtcctgtg ccggggccag ggtcggtcca cacaggacag ccagcggcag
    13681 tagggctgcc aggcggagga caggggcggg acggggcagg gccatggtgt gctccaggat
    13741 cggacgcgcc atcttaaacc gcgatccgct cgccgtgcgc cggcgccccg ggcgacccgc
    13801 agacgagggg ccggggtgcc gctcaccggc ctgccgcggc ggggctttgc ccgatgagcg
    13861 tgtggcaggg catgtcggca ggtcttccga aacggcgaca ggcgcgcgcg ttcgcccggt
    13921 gaggcccttg ccggcagatc ccggacacgg tttactggtc ccgcggcaac tccgatgccg
    13981 ccttcactcg agaggtccgc catgcttccg tcttcaagca tccccgtcgc gctgccggcg
    14041 cacgccgacc ggctggtgtt gctcttccat ggtgtcggca gtcagccggc ggcgatggtg
    14101 ccgctgggcg agcggatcgc ccgggccctg ccggcggcgg cggtggtggc ggtcgccggg
    14161 ccgcagccgt ccgggacggc gggcggccgg gaatggttct cggtggcagg cgtgaccgac
    14221 gccaaccggc cgggccgcgt cgccgaggct ctgccgacgc tgctggacgc ggtcgccgaa
    14281 tggcagcggc ggaccggcgt ggcggccgag gccacggtgc tggtgggctt ctcccagggg
    14341 gcgatcatgg ccctcgcgac cctcgcgcgg gcagacgcgc cggcccgtac cgtggtggcg
    14401 attgccgggc gccttgccga accgcccgca caggtggccc cggccacgac cgtgcacgtg
    14461 ctgcacggcg aggcggaccc cgtgatgccc atcggtctgg cggaggccgc ggtggcgcgg
    14521 ctggcgcagc tgggggcgcg agtgagcttc gaccgttttc ccgggctcgg gcatgggatc
    14581 tcggaggcgc tcgccgccgg tctggtggcg cggctgctgg gcgatggtgc gccggccggc
    14641 gaggtcggtt ccgtgagtgc gctggcaatc cgcctggagg cggagcaggc gatcgtgtcg
    14701 cggccggccg cccagggcgg ggaggaggtc cgcagcctgg cgctcggccc ggcaacgctg
    14761 gtgcgtcgct gcctgcacca cacgccaccg acggccggcg aactggaggc ggccatcgac
    14821 ctcatcgagc cgcagatcat ggccctcggc ctgcatctgc ctgcgggcgc cgtcctgcgt
    14881 ctgtcgggcg actggccggc ggatctccag cggccggcgc cgggactgca ccgggatgcg
    14941 gtggaggggc tcttcagccg cctggcggcc atcgcccacg gccgcccggc cgcgagcgaa
    15001 gggctgccgg cggatccggc ctacttcgcc gcggcgctgc tactgcggga gctgctccat
    15061 catctgggcg cggtgcgcgc ggaggcggcc gcccccgcgc agggcggggg ccctcagcgc
    15121 agcgcgaaca ccgtcacgcc ggccgggccg gagcccttga agaaggccga cacatagccc
    15181 gacacggcgg ccacatactg ccggccgccc acggcgtagg tcacgatgcc gccggccact
    15241 tcaccgcccg tgttgaagcg gtagagcacc ttgccgctgg tggcgtcgag ggccaggaag
    15301 tcccggttga agtcgccggt gaagagcacg tcgccagcgg tggcggtgac tgcggcgagc
    15361 atcggcgtgc ccgaggcgta gcgccagcgg accttgcccg tggaggcgtc gagggcggtg
    15421 agccagccgc gggccttgtc gaacgggtcc atgacgatgc tgccgccgta atagtgctcc
    15481 tcacgtttga actcgggcgt ggtggcggcc ttcttgaagg tggtgcacca gtcgacggcg
    15541 ggcacgaaca gggtgttggt gcgcgggctg taggccgatc cgttccattc ctggccgccc
    15601 agcaggcccg gacaggagcg cgtgccttcg accgtgagcg ggacgtccac gttttcctgg
    15661 gtggtgaagg cgaccgtgta caggacttcg tggctgtccc ggtcgatcag gcggagcagg
    15721 ccatccttgc cgctggcggc gatgacgttg cggcgcacgc cctccacggt ggtggagaac
    15781 aggggcccgg cgtgggtcag gtcccagtca cggatgtcgt ggggaacggc ctggtggtac
    15841 cagagcatct tgcccgaccg cacatccacc gcaagcaggg agttcgtgaa caggttgttg
    15901 cccggccgtg cgtcaccgta gaagtcgggt gccggattgc caaccgccac gtagagggtg
    15961 ccggccttcg catccagcgt gaccggcgtc cacaggctgc cgccgccgta cttgagcgcc
    16021 tcgggattcg accaggtctc ggcacccggc tggcccggat cggggatgaa gggcaggcgc
    16081 cagcgttcgg tgccgtcgct caggttgaat gcgccgaccc agcccttctg gccgaaatcg
    16141 gcaccggcag gaccgtagat caccatgtcc tcgaagatca gcggcggcat gctgaggtat
    16201 tgctgacccg tagagctggc gatctggcgg ctccatgcca ggctgccgtc gcgggcattc
    16261 acggcgatca ggtagccgtc ggcggtgccg cggatgacct tgccgtcctt gatcgccacg
    16321 ccccggttgg tgggcgacag ttcgttgccc ttcggttccc aggtgtactt ccatttttcg
    16381 cggcaggtgg cggcgtcgat cgcgacgatg gagcgggcga tggacaggta catcgtgccg
    16441 tcgtacacga ccgggttggt ctgggccggg ccggcgtcgg ccgaacggta catgcacacg
    16501 gcctgcagcc gggcggcgtt gctgcggttg atctggcgca gcctggaata gcgctggccg
    16561 ctgtagtcgt gcgtcgcgta cagccaattg ctggtatcgt ccgcagccgc gttgaagtcg
    16621 gcagtggtcg ggccgtcgga cgcgacggcg ctgccgcccg tggcgagggc cagggtcagg
    16681 agggtgaggc gcagggtgct gtgcatgatg ttctccgcat cgttcatggg tcgtcccgac
    16741 ggtcgggcgc cgggtttgct gttctgcccc ggcccgtcga gcgcggctcg acgggcgtca
    16801 gggcggccgc agtatgggag caccctctat gtaaaacaag tagttaccgc ttggtaatca
    16861 gcaccagccg gtgatcttca acgagtcagg acgcgatgaa acgagaacat gccgagcccg
    16921 ttccgcagcc ccccgaaggc tgcccgctgg agcgctacct gcacgtggtg tcgggggcgt
    16981 gggtgccccg gatcctgtgg ttcctgtcgt tcggtccgcg acgcttcggt gagctgcggc
    17041 gcgacatcgg aaacatctcg gccaaggtgc tgaccgccaa gctgcgcgac ctggaagggg
    17101 cggggctggt ggcgcggacc gagacccggg gccagacgcg tcaggtggcg tacgcgctca
    17161 ccgaacgggg gcggatcttc gagccggtgt tcgacgcgat ggtcgaggtc tcgcatcgcc
    17221 ttgccgaact ggaggcacgg gaggccgcgt cgaaggattg aggcggtttc tgcggaatcc
    17281 gggcggccgc ccttccgcgc cgccggatta cttcagcttg agcgcgctgc gcatgtccgc
    17341 cgcggtgagg acgccggagc gcgtcagcga gccgccctgg ccgtcgaaca ggtaggtgcg
    17401 cggaagctcg ccgcgccagc ggggatcgag ggcgtgggcg agggcctccg gggattcgtc
    17461 gccataggcg tagcgctcgt cacgggtcag gccggtgcgg gccagcaggg gggcgctgtc
    17521 ggccgtcgcg ggttcggcct cgacggtgac gatgcgcagc tgtgggtggc ggcgggccag
    17581 ttcggccagg gccgcgagat tcttcttgca atgctcgcat tctgccgacc acagggcgat
    17641 ggcggtgggg cgcgtgtggc tgcgcgggtc cgtgaggctg cgggcggcgt gtcggtcgag
    17701 gggctggagc tctgtcgcgg cgtggccggg caggggcgcc gcggcgatga agagggcggc
    17761 gagggcggcg cggagcgcca gggtggcacg gatcatggca gcgggaccac gcggaaggcg
    17821 tcggcggtgc gccagaaggc gtaggcgcgg ccgtcgcggg tgaggaggcg gggctggtcg
    17881 gaggcgccgg cggtggcgtc cagctcctgg ggcgggtcga agtggcggcc gtcgtcgtcg
    17941 gagcgctggg cgcgcaggcg tgtgcgttcg ccgtcgaatt ccttccacag aagcaccacg
    18001 cgacggccga ggacggcgat gtcggcgtgg gcggcggcgg ggccgccgac aggcctctgg
    18061 ccgttcacgc cggcggacgt cgggcggccg taaaagaccc gcccttcgcc atcgcgctgg
    18121 ttgaaccaca cggcgtggcg gacgccgtcg gcagcgatgg cgagggccgg cccctggtgc
    18181 gggcaggcgt ccacccgcca gcggtcgaag gtggcgcgct gcacggcctc gggcgtgccg
    18241 tcggccttca ggcgggcgag ggcgtggtcg cgctcgttgg gggcgaagac gtggcgccac
    18301 agggccacgg gtgtgccgtc cgggtcgagg gcgagggcga tgcggcagca ttcgcagctg
    18361 tgttcggcga gcttgcgctc gggctggaag ctgcgtccgc catcgtcgga gattgcgctg
    18421 tacacgccgg cgccgcgcca gggtttctgg gcgaggcggg cggcttcctg gtcgcgcttg
    18481 tcgatccaca ccacggtgac gcgtccgtcc ggggcgacga tgaggttctg gaagcgatgg
    18541 gtgatctcgg cctggtcacg atggaccgtg atcggcgcgt cgaaggcgct gccgtcgcgg
    18601 ggcaggcggg cgaggcggat ctcgccggtg tagggcttgg gcaggggctt cgtccaggtg
    18661 acgaggagct cgccgtcccg gttgaaggcg atgcgtggct ggctgtcgcc gtcggcggcg
    18721 atggactcgg cttgcatatt gacgtgctgc ggggcggacc aggtggcgcc cagatccggg
    18781 ctgtggcgcc agacgaggtg ggcgccatcc ttggcgacga tatggagctg gccgtcgggg
    18841 ccaaaggcgg cggcggtagc cagttcgatg cgggcgggcc gcgccgcggg gccctgatcg
    18901 tggccttcgt gggccgcggc cggaagagtc agggcagcca gcaacggaaa taggatcggc
    18961 aggatttttt tcatttcttg tgctgaatca atttggatcc gtcaatttcc ggcgggtgtc
    19021 tctttattgc gtggggatgt tctcgccggg ccgatcatgg taccggccta tttgcggtgc
    19081 gcgcaatgtc gtgctcgggt aggcgtcgcc attttgttgg attttatgca aaaaaacagt
    19141 gtcttgtaat tgctaggtac gcgccgcgcg gcgcgctgcg acaattggtc gcagggcggt
    19201 cgcagatcaa tctccggata attctccgaa ttgggagggg aattggcccc gaataagaaa
    19261 ataaggaatt gattgtgaag acacgcctgc gcccgctgcc tgcggccctc atgctgatga
    19321 cgcttctgcc ggcggcgctg tcggcccggg cggcggctga cagcgtagag ctggcccccg
    19381 tcgtgatcac cgccagccgc agcgagaccc gcgtggagga gatgcccctg cacaccacgg
    19441 tgatcgcccg cgaggacatc gagcgctcgc cggcccgcag tctggaccag ctcctgcgca
    19501 gcgtgccggg catgaacttc accggcgtgc cggcgagcca gtccgatccc accggccacc
    19561 agaccagaat gcgcggcctg ggcaatgcca aggtgctggt gctgctggac ggcgtaccga
    19621 tccacgatcc tttctacatg accacccagt ggttcaaggt gcccctgtcc aatgtggagc
    19681 gggtggaaat cgtgcgcggc ggcaattcca gcctgtgggg caacatggcg gtggccggcg
    19741 tggtgaacat cgtgacccgc cgcgtccagg acgacgccgg cgaggtgagc ctgtcggcgg
    19801 gcagctacgg cacctggaat gtggcattga gcaagaacgt cgccgtatcc gaggccctgg
    19861 gcttcaatct gacggtggac gactatcagt ccaacggcta ccagaccacc ccggcggaat
    19921 tcatgtggcg ctttccccac aagcagccgg tggccgtgga gaacaggaac gtccagctca
    19981 ctgcctactt ccgtccgtcc gccgatctga acggctggct gcgcctgggt taccacatcc
    20041 aggaccagga catcagctac cagtacggca gcaacgtgca gaagagcccg gacctggcgg
    20101 ggagcctgac ccggcgcttc gacgacaaga cgcgcctgac agccagcgcc tgggcccagc
    20161 aggtgaagtt cgagaagtac aacggcagca cctgttatta ccagggcggc acgagttgcc
    20221 tcaacagcaa cagcgccacc ctgacgccgg ccaaggtcaa tgaccaggtc gtcgagttct
    20281 acacccagta cggctcccag cgctatcgcg agcagggcac ctcggtggtg ttcagccagg
    20341 atctggcggg gcgcttcagc agctaccagc tcggcgtgga ttaccgcgcc ctgcgcgccg
    20401 aggacgccga gtggttctac accacgccga ccagcccgac ggcacctcag ggtaacctca
    20461 acagcaccac ctggggcaag ggccgccaga ccttcgccgg cctcttcggc cagggcaagt
    20521 tcttcccggt cgatccgctg gaggtgaccc tgtcggcccg ctacgacacc tggcgcatcg
    20581 acgagcgtgc caacctgcgc accacggccg cgggcgtggc gtccggtggc gcgctgcagg
    20641 acggctccaa gaacgccttc aacccgagcc tggccctgcg ctgggagctg tccgacgccc
    20701 tgtccctgcg cagcgccgtg tatcgcgcct tccgcgcgcc cggcttcaac aacctcacgc
    20761 gcacctttgg caccggcacc agcaccacca tcgccaaccc ggacctggcg ccggaaaccc
    20821 tcaccggctg ggaggcgggc gcggacttcc ggcgcggccc cttcaacctg ggcgccacct
    20881 ggttccacta caacatcaag aacatgatcg ccacctacac ggccaccgcc gccagcgcgc
    20941 cggcccaggt gcaggtgatc tgcggcgggc cgaccctgcc cggctgcggc ggctccgcca
    21001 agtactacac caacgaccag gacggccgcg cctacggcat cgagctggtg gccggctggc
    21061 aggtgcgtcc caccctcagc gtagacgcct cctacaccca caccgagacc ttcctgaccc
    21121 gccacggcag catcgtcacc gatcccctca acgtccaact ggtcgccacc ccgcgggacg
    21181 tggccgctgt aggcaccacc tggcagcccg tcgagcgtct cagcacctac accgagctgc
    21241 gctacatcgg cagcatgtac ctggatacca ccagcaatgg cgccacggca cgcctccagc
    21301 agggcgccat tcccctcgtc aatgccagcg tccgttacgc ctgggaccgg cgtaccgacc
    21361 tgttcgtgag cctggtcaat ctcctcgacc gggaatattc cgagaacgcc tacaccatca
    21421 atcagcccta caaccgcacg ctctcgatgc cgcgcacggc caccgcggga gtaaccctgc
    21481 gcttctgaac cgcgcgccgg cacggcttgg acggcacgtc gggcggcact tgacgccggt
    21541 caatgaggcg cccgccggcg cttggctagg atgcaccctg ccaaacggca tgactccgca
    21601 ttccccaacc gagagaactt ccatgaccgc aatccgtatt ctccccgtgc tgctggtggc
    21661 actgcttgct gcctgcggca agtccgaagc ccccaagacc gaagcgccgc cgccggcacc
    21721 cgctgctgcc gccccggcgc cccaggctgc ggccccggcc gccgacaccg ccgatggcgg
    21781 caagggcgcc gaactcttca aaaagacctg cgccatgtgc caccagaccg gtgtggcggg
    21841 cgcccccaag ctgggcgaca aggccgactg gggcccgcgc attgcccagg gcaaggacac
    21901 cctctacaag cacgccatcg aaggcttcaa cggcaacaag ggcgccatgc cggccaaggg
    21961 cggcaacccg gccttcaccg acgacgagat caagaccatc gtggacttca tggtgtccaa
    22021 ggccgaatag gcgcggcgtc actgttcacc tcggcgcaga cccccctgcg ccaagacccg
    22081 gtcagtgatg gccgggtttt tttatgcccc gcgcggacgt catgcctcca gcgtcggccc
    22141 gtgtgcgtag actgtctgtc cgcaccggca tttttaaggg agaccttgcc atggacccca
    22201 tcgcccggac tgccttctac tgctgtggtg tgcgtgccgc cgacgcggcg tcgccccgcc
    22261 cgatttgcgg tgaccacctg gccgcccgct tcatggacga tgaggcccgc gcggtatttg
    22321 cccgtttcgc ccgtttccgg ggcccgaacc tctcgaacgc cgcgcggcac cggctggtgg
    22381 atgattttct gcgtgcgcac ctcgcccggc atcccgatgg gctgatcgtg ctcctcggag
    22441 cgggcttcga cacccgcgcc ttccgtctgc caggcggacg ctgggtggaa ctggaccagc
    22501 ccgcgctgct ggcccgcaag gaacacgcgc tgccggccga caccgcgccg tcgccgctgc
    22561 agcgcgtcgg catcgatttc gccacgcagg atctggccgg ggttctggcc ccctgggccg
    22621 gccatgaaac ggccgtcgtg gtgctggaag gcgtcagcat gtacctcacg gatgcggcct
    22681 tcgccgccac ggcgcgggcc ctgcacgccg tgctgccagg acacacgctg ctctgtgacc
    22741 tgatggatgc ccgtttcgcg cggcgctatg gtggtgggct gcgtcgcgcg atccgcgccc
    22801 tgggcggaga tttcgcgccg gaccgggcgg atccggcggc cttcgtggaa tccctcggtt
    22861 atgcctcccg cagcgtcgcc tccatcccgg cccgcgccgt cgaacatggc accctgggga
    22921 ttccgcgctg gctcttcaat acgctgctgc ggtccctgcg ggatggctac cgtctgcacg
    22981 gcttcgaggc gcggcgcagc ccggcctcac cttcagcgtg agtcgtcggg cgtcggtcgg
    23041 ctgccgcaca ggcgattgac cgcccgcagc ggggagaggg gctgctctgc gtcgtctgcg
    23101 ccggcgtccg acgcgccgtc ctcgttgcgg atgccactct ccgcatcgct ccagccccgt
    23161 accgcctgca cgccggggag caattcggcg ctgtccaggc cggcggcgcg gaggtcctgc
    23221 tcgcacacgt agacggtgcc gccgcgccgc tggatgtcgg ccacggcggt tgccatgtcc
    23281 ggcccggcgc cgcggcgggc gagggcgccg accgcgccgt cgagggccag cagccagaac
    23341 ggccggaagc tgccgggcgg atgttcctgg cgggcctggg cgagggcctc ggacagggtg
    23401 aagccgtcgc cggccacgta gcctccgccg ttgacgacgg ctgccgtggc gctcagggcg
    23461 acgacgctca ggagcaggac aagggtgcgc tggggcttca tcgtgtagcg ggtctccggg
    23521 ggtcaggcct tgccacgggc ggcgaggtgc tcggcgatca gctcggcctg gaagcggctc
    23581 agggtgtccg gatcggtgaa gatggtctgg gtgttgttct gggccttgta gccgggcccg
    23641 ccggcatggc cctgaaccac gaaggtgcct tcgccggatg cggaccagtt ggtggagcct
    23701 tcgccgccga cccggccgtc ggccacgaag cccttggtgt ggctgatctg gtgggtggcc
    23761 gattcgccga tcacgaagta ggtgttgaag gcgtgggggt tcttcgcagc gtcggaatcg
    23821 agcaggcgct tttcgtggac gccgccggcc tggctcttgt cgagggtgat catcatcacg
    23881 acggacggat cgttggcgag ctgcatgagg atgtcgttca gctcctcgtc atcgaagccg
    23941 aacatgttca tgtacaggga gacgctgacc cgcgagagga tgtgcttgag gatctcgtgc
    24001 acgtcgtcgc ggcccacgta gaagaggtgg aagtccttgc tggcgtttgt agcgtaggat
    24061 ttttcctggg tgtattgctc cagatcgagc aggtcgaagc gctgcagggt cttggtggga
    24121 tcggcgatga cgttgctggc gctgacggtg gtggcgggca tgggacacat cctttccggg
    24181 acggggaggg ccggcacgcg cgctggaaca ccgggacgcg ggcgccacct tcacgctagc
    24241 acgcgggctg cgcgaaatcg gccccggacc gtgcccgatc gggactgttg tgttgctgaa
    24301 tgttagtggg cgtgatgcac ctgccgccgg gcctggcggc gatacggacg ggaagggcgg
    24361 tgttcagtgg gcgtgcccgc cggcgaggtg gtggacgact tcgcgggtga tctcctcgta
    24421 ggccagggca cggccaccct ggctgcggat gtgggcgtca gcggcctcgc gggtggagaa
    24481 ggcgggcagg ttggcgtcgt tcatggggcc gcgggcacgc gagccggcga cgaagacggc
    24541 ctttcgggcg tccacccagg ccttgctggc gtaatccgag acccacacgg cgcccacgtc
    24601 ggcggcactg tggcggcggt caaaggcggg catgttgtgc aggaagcgga agagctcggc
    24661 gggggagtcg aactgggcgg cgctctggtc gttgaagacg atctgggcct tccactgggg
    24721 atactgggcc gggtacatgc cgcacacggc gcagcgggcg ttgcgcggga cgggctgggg
    24781 cgccggatcg gcggccgacg ccgggaggat ggccaaaaag gcggcaatgc ccagggcggc
    24841 gaagggcgag cggatcatgg ggcagttccg ggcggggcgc ccagcagcgg atcttcgagc
    24901 agggcgtcca ccacgcggac gaggttgtcg aagggatcgg cgccgcagac ggtgagcgga
    24961 tcggcacgca ggcggccgtc ggcgccgtgg acatgatggg gaaaggtggc gagttcggga
    25021 tggacggggg cggtgtcgat gcgcagctcg gcgtcgcccc agacccaggc gatggcatag
    25081 gcgtcgggcg cggcgatgcg ggcttcgagg atcacgccgt tgtcgaggcg caggagcagg
    25141 gcgtcgagct tgagttcgac gggggcgtcg aagcgcgtgc cgaagcgttc gtcgaggcgg
    25201 gcggcgaggt cggggtggag gctcatggcg gagttttcag cgttggctgt cggccagcag
    25261 ggtggccaga tcgcttcgca cctgggcgtg caggtcggcg aggatgcggg cggagaggat
    25321 gtcgtcgtag gccgggtgtt ccggcagctc gccgcggcgc aggcaggcga gggcctggtc
    25381 ggcatcgccg gcgccgaggc gggcaaggac ttcgtcgcgg gcgccgcgca gtgcgtagag
    25441 gaggcgggcg cgctgctcca cgtccagggc gagttccggg ggcaggttgg cgtagaaggg
    25501 ttccatggtc ggtctcgggc agggctcaca tcttctggtc gtgcagggcg ccgccgtcca
    25561 ggcgggccat gtcgggggtg atggcggcga agggatggac ctggccgccc tgggcttcgg
    25621 cgaacttccg cgcgccggcc tcgctgccga aggaggggat cgtcgggccc atggcgccgc
    25681 gcttgccgct gccgaccacg tagtaggccg tgcgggcgtc gatccaggcg ccctggggcg
    25741 cgtcccaacg cgtcttgtcc aggtcctgca cgaacacggc gcgcaccttg cgggcctgct
    25801 cgggcttgag gtagatcgcg aacatctcgg tggtgtcgca gaaaaattcc ggctcgggct
    25861 ggccgtcgta atgaatctgg gccttggggc cggggtagtc ggcgaggagc atgccgtcca
    25921 gggcgcaata ggtgtcgcgg gtgatctcga cgggtgtggc gccgccgctg cgggcggcat
    25981 cgccgcaggc ggcaaggagg acacaggcgc cgagaccggc agcgaggccg aggcgacgca
    26041 gggggcgggg aatgttcatc gctggaatct ccacagggca agggcgaggg gcgcgacgat
    26101 ccaggcgaac atcacgctcc ccagcacggc cggatggctg agggcttcgg gaaagacggt
    26161 ggcgaggccg tagagggtgc gcacctcgtc caggctgaag acgttgagga cgcggaagac
    26221 gtcggccggg ttgagcagca ggaggtaggg gaagatgctg ccgccgtagt cgccggcggt
    26281 gaccaccaag gcgcccagga ggagcaggtc gaacaccagc acgaagaaga accaggtgcc
    26341 gatggcgagc ccggaggcgc gggtgcggtc ggccgcgaag acggagagca tcaccgccag
    26401 gctgaggaag gcgaagccga gcaggatggc ggagagcatg aagccgccgt agtggaacag
    26461 ggcgttgaga tcgagctgcg gcgacaggat caggcccacc gcgccgaagc cggccacggt
    26521 ggagaaggcc agggcggcgg cgagtccggc gtatttgccg aggagcagtt cgaggcgggt
    26581 gatgggcagg gtcaggagca ggtcgaggga gccccgttcc cgttcgccga cgatggcgtc
    26641 gaagccgagg atgagggcga tcagcgggat caggtagatc accaggctca ccagggaggc
    26701 gatggtgacg tcgatggagc ggaagcccac ggcgccctgc tgggcggcgc cgaagtaggc
    26761 gatgaccagg gcgaagacgg tgaacacgag ggccaccgcg agcacccagc ggttgcggat
    26821 gcggtcccag aattccttgc cggcgatggt gccgatctgt ctgaagtcga tgctcatggc
    26881 gggggcggga ggcaggttac ggagcggcgg gcgttcattg gccgcggtag ccgaagaaca
    26941 cgtcttcgag ggacggttcg cgcacgtgga ggtcgcgcag ggtctcgccc agggtggcga
    27001 ggcgggcgag ggcgggcatc ttggcttcgc ggggcaggcg gatgtgcagg ctgtcgtggg
    27061 tctcgtcgag gcgctcgacg ggcaggccgg cgaggatgcg ggcgacctgt tcgcgcaggc
    27121 cggcgccacc ggcgatctcc agggtgacgg gcaggtccat ggcctcgcgc agggcctgca
    27181 cggtgccgac ggcgcgcacg cggccgtctt cgaggatggc gaggcgatcc acccgttcct
    27241 ggatctcggc caggatgtgg gaggtgagga tcatggtcac gccctgttcg cgcaggtcct
    27301 tgaggatgcg gtagaactcg cggatggcgg cggggtcgag gccggtggtg ggctcgtcga
    27361 ggaagagcag gcgtggcgtg ccgagcaggg cctgggcgaa gccgagacgc tggcgcatgc
    27421 ccttggagta ctcgcggacg cgccgcccgg cggcgggggc gaggccgacg gtgtcgagca
    27481 gcggtgcgca gtcgcggcgc ggcacggcct tcaggtcggc gaagaaatgc agggtctcaa
    27541 ggccggtgag gttgtcgtag aggacgacgt tctcgggcag gtagccgatg cgccggcggg
    27601 tgtcgcggaa gtccgggccg ttcaccgggc ggccgtcgat gaggatctcc ccggcggtga
    27661 cggggagcag acccagcatc atcttgaaga gggtgctctt gccggcaccg ttgtggccga
    27721 tgagcccgaa cagctcgccc gcctgcacgg cgaggtcgac gccctggacg gcctgcacgg
    27781 tgccgaagtg ccgggtgacg ccgcgggttt caacgatagt gcttgccaag ccagtgtctc
    27841 cagtcggggt ggtcggggcg catgcgcggg tgggggtcaa cgatgctggg cgcgcgcagc
    27901 agcgggaact gctgggcgat caggcgcagg gtctggatcg ccgggctggc gaggagcagc
    27961 ttgacaaggg ggtgacgcca ggacaggcgg tccaccaggt cgttggcctc gtagggcacg
    28021 tcgccgaagc cgtcgccatt gcggtcccag cccacgtagt tgctccagtg gttgccgtcc
    28081 ttcgcgcccc aggtctcgtc ccgggcggct acgtacttga cctgttcccg gttggagatg
    28141 aagtcgttgc gctcgacgat gttgcgcacc gagccggccc acagatgcac gccgacctgg
    28201 ttgtccacca ccaggttgcc ccgcagggtg ttgtattcgg cgtcgtagat gaagaaaccg
    28261 cgggcgttgc cggcgaccac gttgttctcg acgatggcgt cctggatggt gcgcagcatg
    28321 atgccgtggt cggaattgcc ccaggcgcgg ttgttgcgca cgatctggtc gcgcacctcc
    28381 atcagcgcca ggccgccgcg gttgaagtag gtgtcgttgt cctcccacag gttgtggtag
    28441 gagttcatgt agtgggtgcc gtagcggctg tggtgcatgc ggttgccgcg gaagaccgcc
    28501 cggtgggaga catccacgta gatggcgtcg cgcacgaagc tgatgttgtt ggccaggatc
    28561 tgcgcgccgg tggtgttgta gagctggatg ccgttgccgc gctgggacga atccaggtcg
    28621 cgcttgccgg taatgaggtt gtgctcgatc accacgtcgt tggccttttc gatccacagg
    28681 ccgaagaggg tgtagctgaa gtcgcagtgg ctcacccgcg cccggtgcgc accgggctgg
    28741 atgtagatgc cggcgttctg ctcgcccagg tcggcaccgg agtcggtgac gatgaagccg
    28801 tcgacgcgca cgtccggcgc ggcgatccgc agcgtgtcgc cgcgcaggcc gccggacacc
    28861 gtgggacggc cttcgccgag gaggttcaag ggcttgtcga tgcgcaggtt ctccacatag
    28921 cggccgcggg ccacgcgcac cgtgtcgccg ggggcggcgc gctggatcgc cgcctggatg
    28981 gagtcgcccg gctgcacgcg ccagtcggcg gcgtgggccg acggggcgcc ggggccgctc
    29041 aatgccgtca atacagtggc cgcgcaggcg gcggcatgac gaacgtggcg gaagaatggg
    29101 gcgaacatgc aggcgtgggg cgtgatggac aacccagtat gggctgtttc cggagggccc
    29161 tgccttgatg tggatgaact ttgaactgaa gcaggcccga ttgttgcagt gcaatgaaaa
    29221 aatagtcgtt gcaaggtcgc aaaaacccgt gcagagtgct gccccagcga gcttcaggat
    29281 gtgacgttgt tgtacggtag tttgcaggat ttgcggcacc tccatcaccg gctgtcttga
    29341 gtttcgcggt tcaacatttt tttcgaggtt caagacatgg ctaccggtat tgtcaagtgg
    29401 ttcaacgacg ccaagggttt cggtttcatc acgcccgatg gcggcggtga agatctgttc
    29461 gcgcatttct ccgccatcca ggcgaagggc ttcaagaccc tgaaggaagg ccagcgcgtc
    29521 gagttcgacg tggcgaccgg ccccaagggt ctgcaggctg cgaacatccg cccggctgac
    29581 tgagcgctgc ccccggcctg agccgggcgg cgtccgtgac gggctagggc cctgcggaca
    29641 cacgaaatcc ccacccggca ttgccgcccg gtggggattt cgttttttgg cctacggtcg
    29701 aggccctgta ttcatatgaa gatatgaata aaagaccacg tcccgggtta tctgaatcgg
    29761 atatatagtg cagccgcgaa aatgtggact gcgccatgcc atcggaatcc agcgttcctt
    29821 gcgtcggcgg gtttgtgaag cccctggatt gacgcagggg atgtcagatt cttttgcgga
    29881 ggatgcaggg catggtgaag cttaagttga ggcgggtgtg cgcggcggtg ggcgccgggc
    29941 tcgggggcgt ctgcgcggca tcggccatcg cagcggcagt gccggaaact gtgacgctga
    30001 atgcggtcgc cgacagttat gtcgatgcca acagctacaa caactacggc aagatcgtgt
    30061 acttcatggt ggggagcacg aaatcggcgt atctccgctt tgatctcagc agccttgcgg
    30121 atcgagacat ccgcagtgcg acgatgaagg ttcgggcaca gaagccgttc tctgacacct
    30181 tcagcgtgat gcatgcggtg gacaacggct ggaatgaata ctcgctcggc tggttcaacc
    30241 gtccttacaa cgtagtctcc gcacaaggcg cactcagtac cgtggctacg accaacgggg
    30301 ggtgggcgac ggtggatgtg accaacgcgg tcagtctgac ccagggacgt ccgctgacct
    30361 tgcagatcag cgggacgaac acgacgccgc tgtggttcga tacgcgggag tccggcaaca
    30421 cgccgaccct gacggtgacc agcgttccga aatccgtgac cctgaccccg gtccaggaca
    30481 gctatgtcga tcctgcgatg ccggacacga cattcgccgg agcgccgtcg atgaaggcgg
    30541 atcccagcgg cgccgaggtg atcatgcgtt tcgatctctc ggcgctgacc gaccggaccg
    30601 tgctgggggg aaccctccat ctgaagaccc cttatggagg gccgcaggat gtacagatct
    30661 acatgccggc caacgccgac ctccagccca acgtcctgac ctataccacg cagccgaccc
    30721 tgctccccga tccggtgtcg gcgccggctg tggccgagcc cgggggcgtg gcgatcgacg
    30781 tgggttcgat cctcaagggc ttgaatgtac gttcgccgct gtcgctggtg atccggtcgt
    30841 caggttccac actggggatt gcgtcaatgg agacggccac gcctccgacg ctggaggtcc
    30901 tataccgaag cgtgagcatc acgtcaacgc cactgtcatc ggtgcccctc aaggtgggca
    30961 tcaacatcga agcgaccaat tattacggca ccgaagtgcc gggcatcgac ctgatgaaac
    31021 agggtcacac gtggtacacg cagtgttatc cgtggcgcga tgccctgtgc agcgcgggaa
    31081 aattcagccg tagcggtggt tcttcgtggg ataccctgga gcaggatgtc ctggaccagg
    31141 acgcgagtgg ctgggtgcgc agtcttccgg cgcccggtga cggcgctacg accggcgtca
    31201 attacacgtc catcgccacc ctgattccga gcagtctgag cgatcgttat cccggtgggc
    31261 gctacatcgt tcgctacgat ggggagggta cgttcagcct gaccaacagc aaggcgaccg
    31321 tgacgcgtaa taccgcgctg tcctcgcccg ggcgggcggt cttcgacatt gtcggtaatc
    31381 cggcgacggc ggttgggtcc gccatgaatc tcagtatcac ggcgacggat cccaatcgga
    31441 ccggcaatta cctgcgcaat atccggatct ttccgcccgg aggggtgtgc agcgacgacg
    31501 tgacgcgcta ttgcaacgcc cagacggggg taccggcgtg ttccacgggg gccatctgcc
    31561 aggatttcga gacggtctat ccagcgaagc ctttcgaccc gcgtttcctg gcaaacatca
    31621 agcgcttcga cacgctgcgc ttcatgggtt accagagcac caacaatgcg gtgagcgaag
    31681 cggagtggag cgatcggacg cgtccggacc attacacgtg gattcgcacg ggttatcacc
    31741 tcaatccgat cgagaatgtc gtggatctgg gcaacaccct gcaccgcaac atctgggtca
    31801 atgtgcccca tcgcgccagc gacgattaca tctaccaact ggcgacggcg gtcaagaacc
    31861 ggttgagtgg cgacctgaag gtctacgtgg agtacggcaa cgagatctgg aacacgatgt
    31921 tctcggcggg aagctgggtc gagcagcagg ggctggcgga atggccctac gcacccgatt
    31981 ccgcgtacgt gaaacggctg caatggcagg gcaagcggac ggccgagacg tgcgacatct
    32041 gggccagcgt gtggggctcc gacaagagcc gggtgatctg cgtcgccggc atccagacag
    32101 ggagtgcctg ggcggccgga tacgtacttg actgtccgct ctatgctgcc gcacacggcg
    32161 gaatcccgtg ctaccagcac aatatgaaag cggttgcggt cgcaccgtat ttcggcggct
    32221 acattgggga ttggagtcac aaggcggccg tggaaagttg gacgctcgat gccgacggcg
    32281 gtctcgggcg cctgtttgcc gaactcttcc agggcgggca attcaccgac agtcctccag
    32341 gcggtgccat cgcttcggcg ctgcgggcgc tcgacaccag tgtggggctg tcgcgtgacc
    32401 ggggtctgca gacggtgtcc tacgaaggcg gtcagcatct caccgcggtg ggcgcgtcat
    32461 tgcagagtcc tgcgatcgtc cagttgctga cccgcgcgaa ccgtgatgcc cggatgggcg
    32521 atgcctacaa aacctatctc gccggctggg tgggaagtgg tgcttcgctg ttcacgcagt
    32581 ggcacagcgt cggcagctac ggcgtgtggg gtaactgggg gctgctggag taccgggatc
    32641 agccggggtc gcccaaattc aacgccgtcc agacatttct ggggacggtc gggcagtagg
    32701 gggttgtcct gccaccggtc cgtcagctgc ggacaggtgg cgtgggggat tcacggtgac
    32761 acggcgtgcg acgggcgttc gtctcacacg acgagcagcc cggcggtctt caggccgatg
    32821 aacagggcgg cgccgatgag caggttcttg aagctcacgt agcagatcag atccatcaga
    32881 ttggcgcggc ggtagcgccg tccccaccac gggccgtcct tcacgtaggg cttggcatcg
    32941 tgacggacgc gcacttcgag cacgctccag ccaaaccacc agccgatgat ggctccggcg
    33001 gaaaggtgcc ccagggccgt gaggatccag gccaccgtgg cggcgagggc cagggtgatg
    33061 ccggcgccgc gcgccaggcc gctgccaccg ctgccgcgtc gccacggcca cagatggtcg
    33121 atgatctcgg cgctcaggcg ggctacgccg cgggtgtcgc cgtagggcgg ctgacgcagt
    33181 tccgtggggt cggcgacggt catggggttt ctccttgacg ggggagcggt gcagggtgaa
    33241 tggggatgaa gtagccgtcg ctgccgatgg gcgtgagggc ctggccggcc ttgccgcggc
    33301 gcttgcgttc ctgggccagg ggcgggcagg cgtggtcgtc gtagtacagc accatgcaat
    33361 ccaggcacag catgcattcg cgggcgtcga tgttgccctt ggggtcgatg gcgagggagc
    33421 cgcagccctt ggcgcaggcc ttgcactggg tgcattccat cttccgcttg agtccgaacc
    33481 agcggaaggt ggtgggcatg gccagcgcgg cgccgagggg gcacaggtac ttgcagaagg
    33541 gccgttcgac gaagatcgac aggcccagca ggccgccggc gaacagggtg tagggccagc
    33601 tgcggttgag caggccgacc aggaaggtgg ttttgaaggg ttccacctcg gccagctgtt
    33661 cggccaggct catgctgaag aaggacaccg cgagcaggcc gaagaagagg ccgtacttga
    33721 gccatttggc cctggcatgg aaggccggcg acgggtggcg ctgcaggcgc ttcaggccga
    33781 ccgcgccggc gagcttgtag agcagctcgg acagcgaccc gaaggggcac agccagccgc
    33841 agaagaagcc ccgcccccac aggaaggtgg tggcgatgat gaagatccag aacaggaaga
    33901 tcagcggatc ggacaggaac agctcccact tccattggaa cagcagggca tggaaccagg
    33961 tgagcacctg ggtgatggac ggctgggcca tggcgtgaaa gccgacgaaa ccgatgctga
    34021 ccacccagcc ggtgtacttg aaggcattca ccggccactt gttgcgatgg gtggcgcggc
    34081 gtacgagggc gtcgcggcgg gcgtaggcgg tggcgatgat cgtgagcagg gcgacgaaga
    34141 ggccgatgtc gagggctttg tccttccaca ccttgagcca ggtggggtcg ggcttgacca
    34201 ccagcgggcg gccgccttcc aggtagcggg cgtcgagcca gtattcccgg tcgaagttgg
    34261 cgaaggtctt ggcgccggtc tcccggtcga tcttgttacc caggaacacc agcttccacg
    34321 gataggcggc ggagaagccc ttgccgcgga tgatgaagat cgccgattcg tcgtaggccg
    34381 gcgcaccggc cgcagacagg ccccacaggt tgatgtagtc caggtcgcgg aaggtgaagg
    34441 cgtccttgtc ctggcgcacc tgcacccggt cgtagaggcc gccgcggacg aagcccgatc
    34501 ctttgaagga atccatgccc gcagtcttga tgatgaagag cgcgtgctcg ccgtccttga
    34561 ggcggctcat caggctggcg tagccgtcgg cgccgagcaa ggcgcggccc agggtgggct
    34621 ggttgaggta accgaaccac agatccatgt aaggccggcc gtcgcccttg aggcccacgt
    34681 cctcgggttg cacggtgagg cgctgcacgc tgccgtcggc caccaggtcg gcccaggtgg
    34741 gcgtggcggt ggaggcagtg aagcgcgccg ccgggcggat caccggcttg aggatgccca
    34801 cctgctgggc gacggcggtg ccgctggtca tcatcacctg gttctgggcg atgacggtga
    34861 cggtggcgcc ggtgatggcg tccaggccga tgaggttgtc ctcggggcgg gatttgccga
    34921 tctcgatgtt gtcaccgacg aacttgccca ggtactggtt gttgaagcgc accagggcgg
    34981 cttcggggat gcccagcagg aggatgggtt cggagtgctt gaggatgcgc acgccggtga
    35041 agtgtccggt ggtgtccatg cccatcaggg tgatgacggg cttgccggaa taggcgggga
    35101 tgtcggtgat gtcggtggac agcatcacgt agccggcgag ccggggcgtg ccgtccacgt
    35161 ccttgtagcc ttccacatag ctgggctggc ccttgcgctc ggagaagcgg gtggcgccgg
    35221 gcagcacatc ggtgcagggg gcgtagtcgc acaggcgcgg cgaggtgaac agttccggcg
    35281 gcagtttgac ttcgtaggtg tcggcctggg ccgcgccggc cagcaggcag gcggctgccg
    35341 tggcaagagc ggcggccagg gttcgcaggg agaacatcgt cggggggctc cggctgggga
    35401 atttatcagc cgattctgat aatccgccgg cgtggccgcc ttaagctgag tcaatttgcc
    35461 gcaggcggcg ggccggcggg catgctgcgg ggatgattcc tccgcctggc ctggccactg
    35521 ccgagtccgt catcgtcccc gcagtatcgc ccgtcgggcg cgcgggcttg accgatgccg
    35581 gatgtgtcga ggggcgtctg aggccagcgt gtgaggggcg ggcgtttcga gcagggcgag
    35641 aacacgcact ttctgatgcg cccggtgaat gtcttgcgcg ggccggcgca tcctgttggc
    35701 acggctgctc agtgtcttcg ccgcggtgct caaggtctga gcgtgcgtgc tcagggtctt
    35761 ggccgcggtg cgcagtgtct gagcggtcgt gctcagggtc tgagcagacg tgctcagcgt
    35821 cttcgcatgg gtgcgaatca tcttggcagc agtgctcagc gtcttggcgg tgctgctcag
    35881 tgtattggca ggcgggcgaa cacgctgcgc aggcgtgcac atcgtctgcg cagggcggtg
    35941 aaggtctttc accggggcgt gaagacgatt cgcatggggg cggatcgtgt ccacaaaaaa
    36001 ggggagacgc ggacgtctcc ccaagctctc ttcgtcaatc gagcggtcgt ggtgtcactt
    36061 ctccacgaac atgcgggtcc gcatctccag gtgcagggcg tggcagaagt gggtgcagta
    36121 gcaccagtac acgcccggtt tgtcggcctt gaaggtgacg gacttggttt cctgcgggtt
    36181 cacgatgaag ttgatgtcgt acttgggaat cgcgaagccg tgggtgaggt cttccacctt
    36241 gtccaggttg gtgaggatga gggtgacctc gtcgcccttc ttcaccttga actcgcgcag
    36301 gctgtaggcc ggggcctggg agaccaggcg cacggtgacc ctgttgccgt tgcgggtgac
    36361 accggaatct ttcgggtcct tggtggcgtt ggggaagtcg tccacgttgt agacctgccg
    36421 ggtcttgatc ttgtcgcgct tgacgatgat gaagtcgtgg ggctcgggat agaccgggtg
    36481 gtcggccagg agcttcatct tctcgccgcg gatgtcgatg agctgctcgt tttcggggtg
    36541 cagcgggccg acgggcagga agcggtcctt ggagaacttg cagcccacgc ccagaaactg
    36601 gccgtcagcc tccttggttt cggacatgga cgcgttgatg tggccgggtt ggtaatgcac
    36661 gtccacgcga tccaccacgt acttggcgtt cttgtcgccg gcgtagaact tgatggcctg
    36721 gtcgatgttc cacttgacca cctgggaatc caggaagagc gtggtgaagc cgttgccgcg
    36781 gccgtcgaag gcggtgtgca gggggccgag gcccagttcc acttcggcga cgatggcctt
    36841 cttgaggtcg tccagcgtgc cgtcgaagta ggccagcacc tggctcagct caatgacggt
    36901 ggcggtgggg gacagcttgc cggagcagat gaagtacttg ccgtccgggc tggcattgac
    36961 gccgtggggg ttcttgggca ccggcacgta gcacgtgagg gcggtcttcg gatccgggtt
    37021 ggcggcgcgg gtgccgtcca ccacgggcac cttgctgtcg ccgatggtgc tggccttgcc
    37081 ggccttgatg gcggcctcga tgcgctcgat gtggaagaac acgcaggcgt cgcgctcggc
    37141 ggacatcatg tcctcgtaat gggcgccgcc ttcggtgttg tactggttgg aggcggccag
    37201 cttgccgtcg aaggacgagg ccaccaggtc catgttgccg tccacccggc actgccagcg
    37261 cacttccatg gtttctgcgt cgatgcaggt gaacaggcag tggtacttgg tggcgtcgtc
    37321 ctcgtcgcgc ccgtcgttgg gctgcggcac gtggaattcg ttgccgcaga agacgcgggt
    37381 ggtgtggttg atggcctgat cgaccgggtc gcgcttgtcc gggaagatgc cgtggaagcc
    37441 ctggatgttc gggatctcgg tgatcttgtc gcactcgaag gtgtcgccct tgatacgcgc
    37501 caggcgaccg tggatcttgt cgttcaccca gaagtacttg ccgtcgtagg tgccgtcggt
    37561 gtacgaaccg tgtacgtggt gggtgtcgcc ggtcttgtac ttgaggctgc cgtcggccct
    37621 ggtgccgatg acggccttgg attcgttggt gatgccccag ccgctcatgc agtcgatgtt
    37681 gaagaccggc acgcgcttga tctcacggcc ggagggcaga ccgaggatgc ggacttcgcc
    37741 ggagtgaccg ccggaccaca cgccgtaata gctgtcgagc tggccggggg ccgggtggat
    37801 gggcagctca ccgccttctg cggccttggc ggcgggcgcc ggggctgcgg gggcggcggt
    37861 cggcgcggcc tcctgcttgc aggcggacag gccgacggac aggccggcgc cggcgagacc
    37921 ggcgagggcg gcggtgttca ggaatttgcg gcggcccggt acgaggtcct ggtcgtcttg
    37981 aggatggtgt tcggtgctca tcgttggtct ccgtgtgtcc cttagcgaac gggaaggtcc
    38041 gggccgcggg tccgggcatc cctgcgacaa gttgtcgcag cggcactcta ggggggccgg
    38101 gggcgaaaaa aattgaccgt gggcaagaat cgccccgccg caacagattt ccagtgagta
    38161 ttagaagcaa ctgatatttc gacgcttttt tacccgcgca gcggcgcacc cggcgcaaaa
    38221 aagccgccgg cccgggtggg cacggcggca acagggaggt gccagaggga gatcggccag
    38281 tcgttcagcg cactggcacg atggtgttgt ggatgccgtc gtgggcgatg acgcggtagc
    38341 ggggcgtgac gccaccttcc gcgggcggga gctggaagcg cggttcgcgc aggccgatgg
    38401 cgagggtctg ccagctcttg ccgccgtcct cgctgctctg cacgctgtag ctgacgaggg
    38461 gcccggcaac ggccgtccag ctcagggtgg cgccttcgtc cagggccttg gacttgagca
    38521 cgctgatctt gccgagcctg ggcggcttgg cggggatgtc ccgctcaccc agcagcgtgc
    38581 cgaaggcgct gacgcggacc ttggcggccg tggtcgagaa gggcacgacg gcgtcgatca
    38641 ggccggtctg gtcggcgccg gcttccacat cggagtcgcg ccggaagggc acggcgtagg
    38701 cggccagcac cttgccgcgg gcgtcgagga actcgatgcg ggcgtcttcc ggtccgacgg
    38761 gcgtggccag gccagtggga atgcggctca tggagacgag gcggccggtc tggcgggtga
    38821 gattgaccgt agccaccacc gagagcaggt cgccgcgctg catcgggatg tcgtcgccca
    38881 cggcgcgggc gttggcaacg ggggctgcgg caacttcccg gtaaccgatc acgggcgtcg
    38941 gtggggtggc ataggcggct gcaggcggtt cggacgcggg ggcggtactc gtcgcgggcg
    39001 aggctgtgcc gtcaatgatg gccggcggcc gcgcgtggcc ggtgaccggc gccgcggcca
    39061 gcatgggcgc ggcacgacgc ggagccgcca ggacgggcgc tgccttagca ccagtgatgg
    39121 gctcgggccg ttcgccgacc tgggccatgg gcggcagggc ggctgaggtc ttgagcgcct
    39181 tgggggccgg cgcaccggcg ggcagggcgt tctcgccggt gaggcgggcg cggatgccgt
    39241 tgtaggtgta gaagctgatc cactggttgc ggcagtaggt catgacgtcg aaccactggg
    39301 cgccgggcag ggccgccatg ggaatgccgt tggcagtgtc gcccacgtcc aggccggtcc
    39361 aggcgccgtc ggggccggaa attgcgccgt tggtgaaggg ttcgtggctg tcgtcggcgg
    39421 agtcgccgca gcccgagccg ggatgcaggc ggccgtaggt gtggcccagt tcatgaccgg
    39481 tgtaccagtc gccgtaggag ccgtcggtgt cccagccgaa gctgccgctc ggcgtgccgg
    39541 tggggccgga ggcgacggcg gtggggtcgg cgctgccagg gatgctggca gcgcagccac
    39601 gcatgaagcc gcccccatcg gcgaccagcc cgaagtagtg ggtgcgcttg tcggtgccgg
    39661 cggccacgtc attggcgcga agcgtggcga gctgggcgtt ggcgtcgttg cagctgaagg
    39721 gccaggcggc gctggcattg acgctcgcgg tgctcatggt gagggtggag acggggtagg
    39781 cgcggccgag ccaggagcgg accagggtgt agtccagggc gcggggggat ttgaccgtgg
    39841 caggcgtgcc gctgctgtag cggaggccca gcacgcggac ccgtagtggt gcggcggtgg
    39901 caaagctgac gcccaccggg ctgctgtcgc agtccgcgca gacgagggcc gcgcccgggg
    39961 agacacgctg gaccgtgatc cggaactggg tggcaccggc gctgcgcagg gcgggaggca
    40021 cttcgaagtt gaggctgctg gccaggttct cgcgcttggc acgcaggttg ccagccagtg
    40081 ccgggtcgag ggtgaccgtg ttgagggagg tgatgctgac cggtgcggcg cccgtacggg
    40141 tggcggtgag cacgccgcgg acggtgaccg ctgcgccacc gttgatgctc acataggcgc
    40201 gggcgatggt gcgtttgtcg gcgacgaggc cgacctcctg ggccatgttc tgcacgctct
    40261 gggtgatctc caggccatcg agggacagtt cgccagggcg tggcagggtg gaacgccagg
    40321 tgtgcacgta gttggtgttg gcgacggcgt ggatgatgtc ggtcttgcgg tcgccgttga
    40381 agtcgccggt gagccacacg ccgttgggga tggcgtagcc ggcccacggg gagaaggtct
    40441 gcacgcggta ggtgccgtca ccgttggaca gccactggtg ggcgtagctg gtgttggcga
    40501 cagcgtggaa gatgtcggcc ttgccgtcgc cgtcgagatc ggcgacacgc cattcgccat
    40561 tggggatgcc gtagccggcc caggggcgga agctgcgtac ggtgaagccg ccgctgccgt
    40621 tggacagcca ggtgttcacg tagtcggcgc ccgcgacagc atggatgagg tcggtcttgc
    40681 cgtcgccatt cacgtcgccg gtcatccact ggccgttggg aatgccatag ccggcccagg
    40741 ggcggaaggt gcccatggtg taggtgccgt cgccgcggga catccaggta tgtacgtaat
    40801 cggtgttggc caccgcgtgg acgatgtcgg ccttgccgtc gccgttgagg tcggcggtga
    40861 gccacacgcc attggggatg ccgtagccgg cccagggacg gaagctcttg acggtgaagg
    40921 tgccgttgcc gttggagagc caggtattca cataatcggc gcctgccaca gcgtggatga
    40981 ggtcggcctt gccgtcgcca ttgacgtcgg caatacgcca ttctccgttg ggaatgccgt
    41041 agccggccca cgggcggaag gtgcccatgg tgtaagtgcc gtcgccgttg gacatccagg
    41101 tgtgcacgta atcagtgttg gcgacggcat ggacgatgtc ggccttgccg tcgccgttga
    41161 tgtcaccggt gagccacacg ccgttgggga tgccgtagcc ggaccaggga cggaagctct
    41221 tgacggtgta gctgccattg ccgttggaca gccaggtgtt cacgtaatcg gcgccggcca
    41281 ccgcgtggat gatgtcggcc ttgccgtcac cgtttacgtc ggccgacatc cactgaccat
    41341 tgggaatggc gtagcccggc cagggggcga aggtgccgac cgcgaactgg gcggcctgca
    41401 gggagggggt gagtccggca aaaccggcaa aggctccggc cagggcgagg gcgaggcggg
    41461 agcggtggcg tctgtggcgg cgagtgtcca tggcggttcc ccttctggag taaggcggcg
    41521 gtgggccgca cggtgggctc cgggcggctg gcgatcactt taggcttagt cgcattttgg
    41581 gtcagcgtca tatcaccagg aatcgagtga catgcgtcac aagggcgtat cccggatggg
    41641 ccgcgccctg acgggcttac caggacgggg cgtgcgcatg atcggaggcg gtcgggcgcc
    41701 ggcccgcggg cgcgcttcga cggggacacg tgtccgatcg ggctgatgcg acgcgggcgt
    41761 tccgccgacg ccggatcagc cgcggaccgg agtgccggcg cgcagcagca ccagcagggc
    41821 gccgtcgccg ccgtcgtggg ggcgggcctg gcagaaggcg aggatgtcct cgcgctgggc
    41881 gagccagccg cgggagaggt gcttgagcac cgggatgcgg ccgggcgagc cgtggccctt
    41941 gccgtggatg acgcgcaggc agcgccggcc gcgcagcagg gcgtcggcga tgaagcgggc
    42001 gagggcggcg cgggcctcgt cgcgggtgag gccgtggagg tcgagctcgc cctgcaccac
    42061 ccagcgtccg cggcgcaggt cggtgaggac gcggcggggc aggccgctgc gcaggtaggc
    42121 agcctcgtcg cccatgtcga ggcggtcctc gaaggtgagc ggggtttcga gggcttcgcg
    42181 caggacttcg cgttcgtcct cttcccgctg ccggggatgc ggtgccggca gcggccggtc
    42241 gaggtctgcc cggttgctgc ccttgagagg cgccacgtcg ccggcgacgg cgcggaacag
    42301 ggcggccggg tcgtgaagcg tgttcaggtc gacgggcgcg ggcgctgcgt cggtgtgcgg
    42361 cgggctgccc aggcgcgtgt cgcggcgctg gtcgctcagg tcggcgcggt tgaagtcgcg
    42421 gatcggggcg atgtcgccca gggcggcccg gaagacggcg gccgggtcgt tctcatccac
    42481 gcgcgacggg cggcgcggag tgtccggcac ggtggattcc ggcgtcttcg ggcggggcag
    42541 gggcgcgggc cgtgggcgcg acaggtcggc gtagtcctcg gccttgagcg gccggacgcg
    42601 gccgacttcg cggcggaaca gggcggcgtc ggtgtcactt gcctgcgccg gcggggctgg
    42661 cgcagccgga cggccaggga cggacgcgct gcgggcgcca ccggcggtct tgagcgcctt
    42721 cagggcgtcc agtccgatgc ggggtttgtc cttggccatg gatgtcgatc gcgagggcga
    42781 aagcacaacg gaccgggcag gccggtccgt tgtgaagggc gccggtgcgc gttcagcccg
    42841 ccagaccgag accgtccagg taacgctcgg cgtccagggc ggccatgcag ccggtgccgg
    42901 cgctggtgat ggcctggcga tagacgtggt cctgcacgtc gcccgccgcg aacacgccgg
    42961 gcacgctggt ggcggtggca ttaccgttgc gtccgccgtg ggtgacgatg tagccgcctt
    43021 ccatttcgag ctggccttcg aagatgtcgg tattgggctt gtggccgatg gcgatgaaca
    43081 cgcccttcag ggcgatgtcc ctggcggcgc cgctgttgac gttgcgcacc cgcatgccgg
    43141 tgacgccgct ggcgtcgccg aggacttcgt ccagggtgga atccagttcg aggacgatct
    43201 tgccggcggc gaccttttcg taaaccttgt cgatgaggat gcgctcggcc ttgaacttgt
    43261 cgcggcgatg tacgagggtg accttgctgg cgatgttggc cagatagagg gcctcttcga
    43321 ctgcggtgtt gccgccgccg atcacggcga cttcctggtt cttgtagaag aagccgtcgc
    43381 aggtggcaca ggccgacacg cccttgccgg agaaggcctc ctcggagggc aggcccaggt
    43441 acttggcggt ggcgccggtg gcgatgatga gggcgtcgca ggtgtattcg ccagcgtcgc
    43501 ccaccagacg catgggcttt tccgccaggt aggtggtgtg aatgtggtcg aacaccatct
    43561 cggtgttgaa gcgcagggcg tgcttctcga agcgggccat gagctcgggg ccctgcacgc
    43621 cgtccgcgtc ggccggccag ttgtccactt cggtggtggt catgagctgg ccgccctggg
    43681 cgatgccggt gatcagcacg ggcttgaggt tggcacgggc ggcatacacg gccgcgctgt
    43741 aaccggccgg accggaaccg aggatgagga gcttgatgtg tcgggtggcc atggcgtgtc
    43801 tctctctggt ctgagcaaag ccgggattat aagtcgggat gccgggtcgg gaggcgcccg
    43861 ctcgagcgga ggcccgcacc acgtcgagat cctgacgcat cgggcatggc catcgacgga
    43921 ttcaagtttg atttatactc tgccgttaag gtgtatgact actccatccg ggtcagcaca
    43981 ccattccttc atgcattggg agaggctcca tgtccgttaa cagcaccgtg tccaccattg
    44041 ccctgcggac gtttccgatc tttcaggggc ttggcgacga ccgtctggcc ggcgtggccc
    44101 gctgtgccat gatgcgccgc gtgccgcggg gatccgccgt ggtgcacgag ggggatcgca
    44161 ccgacttcgt ctacttcgtc ctgaccggca gcctcaaggt catggtgagc gacgaggacg
    44221 gccgcgaggt gatcctcaag atcctcggcc agggcgagat gttcggcgag atgggcgtgc
    44281 tggatgacag tccgcgctct gcgtcggtgg tggcggtgtc gccgtcggat ctggtgacga
    44341 tttccaagac cgacttcaag cgtctcatgc aggaaaactt cgagctcgcc tggcatgtga
    44401 tgtgcaacct ggcccatcgc ctgcgcgacg ccgatcggaa gatcgagagc ctggcgctga
    44461 tggacgtgta cggccgcgtg gcgcgcctgc tcctggagat ggcggaagag gtggatggcg
    44521 tcaaggtcgt gcgcaagaaa atctccaagc aggacatcgc caagatgatc ggtgcctccc
    44581 gcgaaatggt cagccgggtg atgaaggatc tgggtctgcg cggcctgatc gaggagacgg
    44641 acggtggtgt catcctgcgg gagaagatct acgatttgtg aatgccccgc aaatccctca
    44701 ccgcgctgat cgctcggctt gcggaggagg gggcaaccag agaccctggt ttataatcaa
    44761 ggcgcggtcg ggtaccgcgc tttttgtttt ttcgacgctt cccgcgaccc ttgccgtccc
    44821 atgctgaacc gcccctcgac ccgtcccgtc ccgctccccg aaaagatcgc cagcctcctg
    44881 caggaagcac gctggctgct gctgggcgtg ctcgcggcgt atctggcgat cgtgctcttc
    44941 ggatacgcca agagcgatcc cggctggtcc catgcggtgt acgtggagcg tgtgagcaat
    45001 ccgggcggac gcttcggcgc ctggctgtcg gatctgctgc tctatctctt cgggctgtcg
    45061 gcctggtggt gggtggcctt cctcggggtc ggtctggcgg tcggattccg tcgtctgcaa
    45121 cgggagcatg gggcggaccg gcgttcgctc ttcatcgtgc tggcgggctt cctggtggtg
    45181 ctggtggcca gtagcggtct ggagtccatg cgtttccatt ccctgcgggc caccttaccc
    45241 ctggagccgg gtgggctgat cggcgtggaa ttgggcagtg tggtcggcat ctatctcgga
    45301 ttcacgggcg gcacgctgct cttgttgacc ctcatcgctg ccggcctgag cctgttctcg
    45361 ggcgtgtcct gggtcgaggc ctccgaggcg gttggcgggg cgctggaggc tgcctggaac
    45421 ttcgtgctgc acggcgccca gacctgggag gatcgccgcg tcggccgcga agtggcgcag
    45481 aagcgcgagg ccgtcgtgga gaccaagcgc aagaaggagg aagcggcacc gcccccgccc
    45541 gtgcgcatcg aaccggcggt ggtcgaggtg ccccgttcgg agcgggtggt caaggaacgc
    45601 caggccgccc tgttcgtcga gctgcccggc ggcagcctgc cacccctggc gctgctggac
    45661 gagccggccg acgatgtgga gccgccctcc gtcgagtcgc tggaattcac ctcccgcctc
    45721 atcgaacgga agctcgccga tttcggtgtc gaagtgaagg tgctggccgc ctatccgggg
    45781 ccggtgatca cccgtttcga gatcgagccg gcggtcggcg tgaagggcag ccaggtgatg
    45841 aacctggtca aggatctagc ccgcgccctg tccgtggtga gcatccgcgt cgtcgagacg
    45901 gtgcccggca agacctgcat ggccctggag ctgcccaacc ccaagcgcca gaccgtgcgc
    45961 ctgtcggaga tcctcggttc caaggcctac cacgacatgc attcgcccct cacggtggcg
    46021 cttggcaagg acatcggcgg ccagcccgtg gtggccgaca tcgccaagac accccacctg
    46081 ctggtggcgg ggacgaccgg ctcgggcaag tcggtgggca tcaacgcgat gatcctgtcg
    46141 ttgctttaca agtccgagcc ggaggacgtg cgcctgatcc tggtcgatcc gaagatgctg
    46201 gagctgtcga tttacgaagg cattccgcac ctgctggcgc cggtggtgac cgacatgaag
    46261 cacgccggca acgccctcaa ctggtgcgtg gccgagatgg agaagcgcta caagctcatg
    46321 tcctcggtcg gcgtgcgcaa tctggcgggc tacaacaagg cggtggccga ggcggtcaag
    46381 gcgggtgcgc cgctgaagaa ccccttctcc atcacgccgg aaagccccga gccgctggaa
    46441 aagctgccct acgtggtggt catcatcgac gaactcgccg acctgatgat ggtggtgggc
    46501 aagaagatcg aggaactcat cgcccgtctc gcccagaagg cgcgggcggc cggcatccac
    46561 ctcatccttg ccacccagcg ccccagcgtg gacgtgatca ccggcctcat caaggccaac
    46621 atccccacgc ggatggcctt ccaggtgtcc agcaagatcg actcccgcac catcctcgac
    46681 cagatgggcg ccgaggcgct gctgggcatg ggtgacatgc tctacctggc acccggcacc
    46741 ggcctgccga cgcgcgtgca cggcgccttc gtggccgacc acgaggtgca caaggtggtg
    46801 gaattcctca agagcaccgg tgcaccggac tacgtggagg gcattctggc gcctcaggac
    46861 gaggaggcgg gcgatctgct cggcggtggc gaagggggcg agggcagcga ggccgatccc
    46921 ctctacgacc aggctgtgga ggtggtgctc aagacgcgcc ggccgtccat ctccctggtg
    46981 cagcgtcacc tgcgcatcgg ctacaaccgc gccgcccgac tcatcgagca gatggagcgt
    47041 tcgggcgtgg tgtcggccat gagcgccaac ggcaatcggg acgtgatcgt gccgccccgc
    47101 gaaggagaat gatgatccgc aagatgtcct gcgccgccct cgggctggtc gcgctgttgt
    47161 ctgccggggc ggcccgtgcc gatgggctgg cccagctgaa gcagttcatg gaaggcaccc
    47221 gcgcggcgaa gggcaccttc acccagcagg tgttcgccaa atccggccac aagccccagc
    47281 aggccggggg cacctttgcc ttttcccggc cgggcaagtt ccgttgggtc tatgacaaac
    47341 cctatgccca ggtgctggtc ggcgacggcc gtaccctgtg ggccttcgat ccggagctga
    47401 accaggtcac caccaagaag ctgggtcagg ccctgggcag cagcccggcg gccatcctgg
    47461 cgggcgacaa tgcgctggag cgcagcttcg tgctcaagga cgccggcgtc gccgacgggc
    47521 tggagtgggt tgaggccacg cccaagggcg aggacagcag tttcgagcgc atccgcctgg
    47581 ggctatctgg tgggcagttg cgggccatgc tgttgttcga caactttggc cagaccacct
    47641 cggtgcagtt cagccagatc gagcgcaatc cggcgctcga tgccgccctg ttccacttcg
    47701 ttccccccaa gggggccgac gtggtgggcg acgggagccc cgctggcgca gcccgctagg
    47761 cagcaggatg gccgatctct tcgatgccct cgagtcgccg cgggtgccgc tggcggaggc
    47821 gatgcgtccg cggacgattg ccgacgtggt cggtcaggct cacctgctcg gccccggcaa
    47881 gccgctggcc ctggccttcc agtccggcaa gcctcattcg atgatcctgt ggggcccgcc
    47941 gggtgttggc aagaccaccc tcgcgcggct catggcccag gccttcgacg cggagtttgt
    48001 tgccctgtcg gcggtgttct cgggggtcaa ggacatccgt gaggccatcg cccgggccga
    48061 ggtggagcgc gcccgcggcc ggcacaccat tctctttgtg gacgaggtcc atcgcttcaa
    48121 caaggcccag caggacgcct ttctgccctt cgtggagcag gggctggtca cgctggtggg
    48181 ggcgacgacg gagaatccgt ccttcgaggt gaattcggcg ctgctgtcac gggccgcggt
    48241 gtatgtgctg cagccgctca cgcccgagga actcgtcgtc ctcttcgagc gggcgcgggc
    48301 ccacgcctgc ccgcagctcg ccttcgagcc cgacgcccgc gaccgtctcg tcggctttgc
    48361 cgatggcgat gcgcggcgtc tgatgaatct gctggagcag gtacacaccg ctgcggatac
    48421 ggccggacag aacccggtca cgccagcctt cgtggacgaa gccctgtcgc gcaacctgcg
    48481 gcggttcgac aagggcggcg aggccttcta tgaccagatt tcggcgctcc acaaatccgt
    48541 gcggggctcc gatccggatg catccctcta ctggctgtgc cgcatgctcg atggtggggc
    48601 ggatccccac tatctgggac ggcggctggt gcgcatggcg gtggaagata tcggtctcgc
    48661 ggatccgcgg gcgctggaga tcagcctgaa cgcgtgtgcc acctacgagc gcctgggcac
    48721 cccggaaggg gagctggcgc tggccgaagc ggcggtcttt ctggcctgcg cggccaaatc
    48781 gaacgcggtg tacaaggcgt ataatgcagc ccgcaagttc gtggcggccg acgcatcgcg
    48841 tccggtgccg atgcacctgc gcaacgcgcc gacacgcctc atgaaggatc tcggctacgg
    48901 caaggcgtac cgctatgccc acgacgagcc ggacgcctac gcggcagggg aaagttatct
    48961 gcccgacggc atggcgagac cagactggta tgtcccgacc gggcgcgggc tggaagcgca
    49021 gattgcggac aagctggcgc gcctgcgcga aagggatcgc gcggcgcagg atgcggacag
    49081 gcgggacgaa gtctaatggg tgtgcgacac cggatgcgcc tggatgaagc gtgcgcaggc
    49141 ggctcggaag cgattggcgt tggcccggtc gccccgttcc tcggcccgat ccaccagatc
    49201 cagcaagaac tgggtgagca tcagcgtgcc ttccggggtc tggaccagcc cgcaggcgat
    49261 ctgggcggcg gcggcttcgg gaatgtcgtg gtgctcggcg atgatcgcga cgtcttcatc
    49321 ggtcaagtcg cagtaatcca gacagtcgcg tatggatagc attggagtct ccttcgatgg
    49381 tcggacgagg aagggttcct tctgccggga gccttcttca attgtgacct ctgaatacac
    49441 gaattcaaga aaagtttcac agcgggcagg ctatgaactt tccaatttcc attgaacgag
    49501 cccgtggaat gcgtcacgcg ctttgtgatg cgtcgcaata tagcggaaac gttcgcggtc
    49561 tgccaagcaa tatttatgcc gaatatgatg tcggtcggat tttcgatttg aataggattc
    49621 cccatgcttg acgtacaact gctgcgcacc cgcctggatt ttgtcgccga acgccttgcg
    49681 acccgcggcc tgcaactcga tgtggcggcc ttccaggcgc tcgaagacga acgcaaaacg
    49741 cgccagaccc gcacccagga actccaggca cggcgcaatg ccctgtccaa gcaggtcggc
    49801 cagctgaagg gcaagggcga ggacgcctcc gcggtgctgg ccgaggtggc gagcatcggt
    49861 gacgaactca aggccaacga gattgccctg gccgagctgc tggagcgctt caacgccttc
    49921 gtcgccgggc tgcccaacct gccccacgag agcgtgccgc cgggcggttc ggaggcggac
    49981 aacgtggagg tcagccggtg gggtgcgatc ccgaccttcg actttgaggt gcgcgaccac
    50041 gtggacctgg gcgcccgcct cggcgggctg gatttcgaga cggcggccaa gatctccggt
    50101 gcgcgcttca ccctgatgaa gggcggtctg gcccgcctgc atcgggctct ggcccagttc
    50161 atggtggaca cccacaccgc cgagcatggc tacaccgagc tctacgtgcc ctacctcgtg
    50221 aatgcggcca gcatgttcgg caccggccag ctgcccaaat tcgaggaaga cctgttcccg
    50281 gtgccccgtg ccgacggggg caagttctac ctgatcccca ccgccgaggt gccggttacc
    50341 aacatcgtgc gcgacgaact cctgtcggcc gatgcgctgc ccctcaagtt cgtcagccac
    50401 accccgtgct tccgctccga agccggcagc tacggcaagg acacgcgcgg catgatccgc
    50461 cagcaccagt tcgacaaggt ggagctggtc cgcatcgagc atcccgacca ggcctgggcg
    50521 gcgctggagg aactcaccgg ccacgccgag gccatcctgc agaagctgga actctcctat
    50581 cgcaagatcg tgctgtgttc cggcgacatg ggcttttcgt cggccaagac ctacgacctg
    50641 gaggtctggc tgccggccca gaacacctac cgggaaatct cctcctgctc ctgcttcgag
    50701 ggcttccagg cacgccgcat gcaggcccgc ttcaagaacg cccaaggcaa gaacgaagcc
    50761 gtggcgactc tcaacggctc cggcctggcg gtggggcgca ccctcgtggc ggtgctggaa
    50821 aactaccagc aggcggacgg ctcggtgcgc atcccgacgg tgctgcagcc ctacatgggg
    50881 ggcgtgacca tgctggcgcc gatcaactga tccggcagcg tgatcgaatt cctttgtcct
    50941 cggatttaaa tgacaggtgg gagtcttgcg ttgacccgaa gagggggtgg gctgatatcg
    51001 ttttgacatt ctttcacaac aaaacgatga ggcaccccca tgacgagcaa atcctttcag
    51061 accattacgg ccgatctgtc ccgggcggtg gacagcctgc gtgccgagat ccccggtacg
    51121 atgcagggct tcagcagcat ggcccgcgac gccatgaagc ccggtgtcct gtccgcgctc
    51181 gacaaggaac tcctggccac cgccattggc gttgcggccc gttgtgacgg ctgtgtcggc
    51241 ttccatgtca aggcgctgat ccgcctcggc gtgacacgcg aacagctgat ggagaccctt
    51301 gcggtcgccg tgtatatggg gggcggcccg agcctgatgt acgccgccga ggccgtgcgc
    51361 gcctacgacg aattcatcga tctgccgatt cgccaggcag cctgagctgg cgttccaggg
    51421 atgctccgct cctcgtatca cgctggcgtg atgtccggct cgatggcgcc cgcccgtgtt
    51481 ttgaagcgcg cgtaaggcgt cgaggggcgt ggccgcgctg ccgcagcaca tccgccggac
    51541 ggcccccttt cgacattccc cgcggtatcg ggactgacat tcccggacga ttcggttaca
    51601 atcgacagct tcgagcggaa aggtgccaga gtggtcgaat gggccggact cgaaatccgg
    51661 tgtacagctt gctgtaccgt gggttcgaat cccacccttt ccgccaagca tcccgtaaaa
    51721 acgcgccttt cggcgcgttt tttcgtttct gcgatcctgt ggtgtcggtc gcagggcgcg
    51781 tccgcgcctg cgaccgggtg ccctgcatgc tttcagtggg tggcgtacac gctgttgcgc
    51841 cagccgccgc cgcaggtttc ggtgctgttc gcgatgcatg gcgtattgca ttcggcgtct
    51901 gcagccttgg tgggcggcaa cgtgttgccc gcgtagcact gaccttgcca ttgcaggccg
    51961 gcgtaggcgt agccttcccg tccggcggcg gcgacgcagg attccacggt agcgccggag
    52021 gacatcaggg cgatcggcag ggtccggctg gcggtatcca cgtagcagcc cttgtaggcg
    52081 ttggcgggcg gtttcggcaa gctcggaaca cctgtcgcgt agacgctgtt gcgccacgtg
    52141 ccgccgcagg tctgaccggc gccggcggtg cagggcatgt tgcactcgtt gtccgccacc
    52201 ttggtcttgc cgaggctgtt gccgccgtag cactgaccac catattgcag gccggcgtag
    52261 gccagattgc gggcacgggc ggcggcaacg caggtgtcga cgttggcgat gctgttggcc
    52321 atcaaggtga cgggcaatgc ccgactggca tcgtcggtgt agcagccctg atagaccggc
    52381 ttggcgggcg cggcgggtgt gacgccggtg gcgtacacgc tgttgcgcca gcttccgccg
    52441 caggactcgg acttgtcggc cgagcagggc atcgcgcatt cgctgtcggc gaccttgcgg
    52501 agggccggag cgttcccggc aaagcaggtg ccgccgtact gcaggcccgc gtaggcgtag
    52561 gtgtgggcct tggctgccgc catgcagctt tccaccgtgg cgcccgcggc catgagctgc
    52621 accggcagcg cgcgcacgcc gtcatccgtg tagcagccca cgtaggcacc ctgggtttgc
    52681 ggatatgtct gcagattgtc gaacacgccg tagataccct ggccgttcac gcgaccgcgc
    52741 tgaccgggcg ggttggcgat ggacgagaag aggacgtagg cgatgtcggc gctcgggcgt
    52801 tcgacggtca gtgtctgcca cgtgccccag ccggctttgc cgtcgccgta ccagttggcg
    52861 taatacacct tggccaggag cttgccgctc gcgtcgaagg cttccaggaa ggggcgccgc
    52921 tgcaggctgg cgtcgttgcc caggtattcg atggcttcca cgccccgtgc atcgatgctg
    52981 accttcttct gcggggctgc gaatacggcc ttgacggccc cctcgtcggc cgaaaaggcc
    53041 gacgagaaag cgccgcccgt ggcgggattc cacagggtga cggtgttcga gttctgccac
    53101 gcagaggcgt aggcgtgacc gctctggctg gcaacgctgg aaaggaggac gccgctgccg
    53161 gcgtaggttc catccaccag ggtgcccgac gggacatcgt cgaagccgat gtccgccgcc
    53221 cacgcgggtg ggctgcctgc aagcgccgtg aggaccagca ggtggatcag ggattggcga
    53281 gtgtgcatga catcctccgc ttcgattcgt tggggaaagc gggcctggcc gggccatgcg
    53341 acgcggcaag gcccaagggg tgactgacag gttcacggtc ggcccggcct gcaggggtgt
    53401 cgttgacccc ggtcaaccct cggtgtggcg agcgctgttc atgtacccat tccgggtttc
    53461 aggcgcagcc aggttacagg gccgggtcga gcggtcgcgc cggggcttca gggcggatgc
    53521 gcgcgtcatt cccgggcgcc gtgcgtgcct gcgggcggct acgctcaccg gaaagtagcc
    53581 ttccgctttg ctggcagtgc agcttgcaag gacggcccgg cggacgccgg tgcatgcaga
    53641 caagaccctg atcgaaaagc ggttttatgt ttcgcatggt gcgggcacgg atcttgtgtc
    53701 ggtgtctcct cgcgatgcgg caaacgaccc cgccacaagc ggtcggccgc gcacccacat
    53761 gacgttcaga cgcgattcat ccgcgagggg agccgggttc catgactgaa accttttacg
    53821 acgtgctgcg ccggcagggg atcacccggc gcagcttcat gaaattctgc agcctgacgg
    53881 ccacgtccct cgggctgggc agcgcctttg tgccgaagat tgcccacgcg ctggaaacca
    53941 agccgcgcac gccggtgatc tggctgcacg gcctggaatg tacctgctgc tccgagtcct
    54001 tcatccgctc ggcccatccc ctcaccaagg acgtggtgct gtccatgctg tcgctggact
    54061 acgacgacac gctgatggcg gccgccggcc accaggccga ggcgatcatc gaagaggtca
    54121 agaagaagta caagggcaac tacatcgtcg ccgtggaggg caatccgccc ctcaacgagg
    54181 acggcatgtt ctgcatccac ggcggccggc ccttcgtgga ggtgctcaag gagacctgcg
    54241 ccgacgccaa ggccatcatc agctggggcg cctgcgcatc ctacggctgc gtgcaggcgg
    54301 ccaagcccaa cccgacccgg gcgacgccgg tgcacaaggt gatcaccgac aagcccatcg
    54361 tgcgcgtgcc gggctgcccg cccatcgccg aagtgatgac cggcgtggtg acctacatgc
    54421 tgacctttga ccgcatcccg gagctggacc gtcagggccg cccgaagatg ttctacggcc
    54481 agcgcatcca cgacaagtgc taccgccggc cccacttcga cgccggccag ttcgtcgaga
    54541 gctgggacga cgaggcggcg cgcaagggtt actgcctcta caagatgggc tgcaagggcc
    54601 cgaccactta caacgcctgc tcgtccatgc gctggaatgg cggcgtctcc tggccggtcc
    54661 agtccggtca cggctgcatc ggctgttccg aggaaggctt ctgggacaag ggcagcttct
    54721 acgaccgcgt caccgacatc aagcagttcg gcatcgagag caatgccgac aaggtgggcg
    54781 gcgctgcggc cggcgtcgtg ggcgcggccg tggccgccca tgcggcggtg acggcgctgg
    54841 cccgggcgcg ggcgaagcag ggtgaagatc agaatgcggg aggtcaatga tgactgtgct
    54901 tgaaacccag ggcttcaagc tcgacaactc gggcaagcgc gtggtggtgg atccggtgtg
    54961 ccggatcgag ggccacctgc gtgtggaagt gaacatcgac gacaagaacg tgatccgcaa
    55021 cgcggtgtcc accggcacca tgtggcgcgg gctcgaggtc atcctcaagg gccgcgaccc
    55081 gcgcgacgcg tgggccttca ccgagcggat ctgcggcgtg tgtaccggta cgcacgccct
    55141 cacctcggtg cgcgcggtgg aggatgcgct ggcgatccag atcccggaga acgccaacat
    55201 catccgcaac ctgatgcagc tgaacctcta cgtccatgac cacctcgtgc atttctacca
    55261 tctgcacgcg ctggactggg tggacgtggt gtcggcgctg aaggccgatc ccaagaagac
    55321 ctccgagctg gcccagagca tctcgagctg gcccctgtcg tcgccgggct acttccgcga
    55381 cgtgcagaac cgcctgaaga agttcgtcga atccggccaa ctgggcccct tcatgaacgg
    55441 ctactggggc aacccggcct acaagctgcc gcccgaagcc aacctgatgg cggtgaccca
    55501 ctacctggag gccctcgact tccagaagga catcgtcaag gtccacacca tcttcggcgg
    55561 caagaacccg catcccaact ggctggtggg cggcgtgccc tgcgccatca acctggaagg
    55621 cacgggcgcg gtcggcgcga tcaacatgga acgcctgaac atggtcagtt ccatcatcga
    55681 ccgctgcatc gacttcatcg acaacgtcta catcccggac ctcaaggcca tcgccagctt
    55741 ctacaaggac tggacgtacg gcggcggcat ctcgtcccag gccctgctgt cctacggcga
    55801 cattccggac aaggccaacg actacaccgc caagagcctg ctcctgcccc gcggcgccat
    55861 catcggcggc gacctcggca agatccacga ggtggatctc aaggaccccg agcaggtgca
    55921 ggaatacgtg gcccacagct ggtacaagta cgccgacgaa tccaaggggc tgcacccctt
    55981 cgacggcgtc accgaacccc acttcgagct gggcaaggcc accaagggca ccaagacccg
    56041 gatcgagaac ctggacgaag ccggcaagta ttcctggatc aaggccccgc gctggcgcgg
    56101 caacgccatg gaagtcggcc ccctggcccg ctacgtgatc ggctatgccc agaagaaccc
    56161 ggaattcaag gagccggtgg acaagctcct gtccgacctg ggcctgcccc tgcaggccat
    56221 cttctcgacc ctgggccgca ccgcggcgcg gggcctggag gcatcctggg cggcccacaa
    56281 gtcccgctac ttcttcgaca agctgatggc caacctcaag gccggcgacc tgaccaccgc
    56341 tcacatcgac aagtgggatc cgtcccggtg gcctgccgag gccaagggcg ccggcttcac
    56401 cgaagcaccg cggggcgccc tggggcactg gatcaagatc aaggacggcc gcatcgacaa
    56461 ctaccaggcc gtggtgccca ccacctggaa cggcggtccg cgggacgaca agggccagat
    56521 cggcgccttc gaggcgtccc tgatgaacac gccggtggcc aagccggacg agcccctgga
    56581 gatcctgcgc accctgcatt ccttcgaccc ctgcctggcc tgctccaccc atgtgatggc
    56641 gccggacggt caggaaatgg cgtcggtcaa ggtccgctga agaggaggga cgcatgcgct
    56701 ccacgtccat cgcctgtgca catcccggtc gacgcggcat cgccggccgc ccgcgcgggc
    56761 acgccgcagt gtttcgtgaa tcccctgcca cgccccgtcg ggaggcctga ccatgctttc
    56821 acaacaagac atgctggagg ccgaacggca cggccgtcag gtccgctcca tctacgtgta
    56881 cgaagcgccg gtgcgcctgt ggcactggat caacgccctc gccatcgtgg tgctggccgt
    56941 caccggctat ttcatcggca agcccctgcc gacgatgccc ggtgaggcgt cggacaactt
    57001 cctgctgggc tacatccgct ttgcccactt cgccgcggcc tacgtgttcg cggtggggct
    57061 ggccgggcgg gtgtactggg ccttcgtggg caaccaccac gcccgtcaga tcttcaagct
    57121 gcccctcacc aacgcccagt ggtggagcga agtcttcttc gaactgcgct ggtatctctt
    57181 cctggaaaag cacccgaaaa agtacgtggg ccacaacccc atggcccagc tgatgatgtt
    57241 cttcttcttc accgtgctgg ccttcgggat gctcttcacc ggcttcgccc tctacagcga
    57301 agggctgggc ctcggctcct gggccgaccg cctgttcggc tgggtgatcc ccgccctcgg
    57361 cggctccatg atgacccaca gcctgcaccg cctgggcatg tggatcatga tgagcttcgt
    57421 cgtggtgcat gtgtacgccg ccatccgcga ggacatcatg agccgccaga gcctgatctc
    57481 cacgatggtg tccggctggc gcatgttcaa ggattgacgc atcggggcgc ctccgccccg
    57541 cgtatccctt tccccgccgc ggccgggtcg cctcccttct tgggcggtcc ggccgcaact
    57601 tcatgggtgc ctgcatgatt cgatgcttcc gttttcagag caaactgtaa gtctggtttc
    57661 cgcttgcagg ggcgttgacc gcccatgacg acgggaaggg gctgtatctg ctgccatttt
    57721 tccgccgaaa tggcagggcg gtcgcctggc acgggtcttg ttaaggagtc atgcctttcg
    57781 gactccggat tcccatggac acctctcccc gcgttctcgt gctcggcatc ggcaaccttc
    57841 tgtgggccga cgaaggcttt ggcgtgcgct gcgtcgaagc cctcgccgag cgctacagcg
    57901 tgccgcccca ggtcaccctg atggacgggg gcacccaggg cctctacctg ctgccttacg
    57961 tggaggaggc cgactgcctg ctggtcttcg acgcggtcga ttacggcgac ccgccgggca
    58021 ccctgcgcat cgtcgaaggc gccgaggtgc cgcgcttcat gggcgccaag aagatgagcc
    58081 tgcaccagac cggcttccag gaagtgctgg cctccgccga gctgctcggc cggctgccgc
    58141 gggaactggt cctgatcggc tgccagccgg tcgagctgga agacttcggc ggcagcctgc
    58201 gggacgcggt caaggcccag atcggccccg ccctcgacgt ggcggcggag ttcctgcgcg
    58261 gctggggcgc cgcgccgctg ccacgtgccg acgacgcccc gcgcctcaac gccggcagcc
    58321 tgggcatggc cgcctacgaa gccggccgtc ccagcgaaca ggaggcgtgc cgcttcggcg
    58381 acgcgcgctt cctggcggga gactgaacat gtgcatgggc atcccctgcc gcgtcgtgga
    58441 cagccagggc tggaccgccc tgtgcgacgg cccggccggc ccggtcagca tcgacctggc
    58501 cctcgtcggc ccccaggcgc ccggcacctg gctgctcacc ttcctgggcg ccgcgcggga
    58561 agtcatcagc gccgagcggg ctgcccaggt gggcgacgcc cttgccgcgc tggaggcggc
    58621 cctcgccggc gagaccgacc gcatcgacgc cctctttggc gacctggtgg ggcgcgaacc
    58681 cactttgccc gaacacctgc gaaaggaatt gccgtgaccg tccattttgc cccggcgccc
    58741 agcgccacgg agcgcctgga ggcggccctc aggcgcctca ccgagcgcca cgccttcacc
    58801 cgcgtggatg cggccttcga accgcctgcc ggcacggcgg tgctgctcct cagcgacgac
    58861 ccccagcgca gtccggaagc cctcgacgtc atcgtcgtgc tgccggaagc cctgcgcgcg
    58921 ctgggccggc ccttgtccgc ctgcgtcgcc gacgcagagg ccagcgccgt gctggcccgc
    58981 cgtttcggcg tcgcccgtct gccggccgtc gtggccttcc gcgacggggc ctggctgggc
    59041 gccttctccg gcatcctgga ctgggcaccc ttcgtcacgg aactggcccg gctgctcgac
    59101 gcgccgccgg gccgtccgcc catttttgtc gcgtccgccg aggcccgtcc atgaagccct
    59161 tcccgatccc cgtggtgccc gttggccccg gcagccagac cgacgaggcc ccggactacc
    59221 tggccatgcc cggtggcatg gccacctttc aggccccgcg cctgcccgaa ggcgcccagg
    59281 cggcggacgt ggcccatgcc gcgtccctgc tggccgggct ggtggacgcc atggccggtg
    59341 ccgccttcga cggcccgcct ctggttctgc cgctggatgc cgagacgccg ggcgtgcggg
    59401 aggtattgac ccagtccctg ggcttcggcg aagtctctgc cttcaccgtc gcaccgaccc
    59461 gcctgcgcgt gcaggagacc gccttcgcgt ccctgtggcg ggtgctggag gagggcgagg
    59521 acggccgcct gctccacgac cgcctcacgg tctgcccggt gccgccggag ctctacgccg
    59581 ccatgcgtgc cacggcgcgg ccggatctcg ttccgcctgc tgtgctgccc ggcatgatga
    59641 acggcgaggc cctcctcgcc gaagtgcgcc agcaggccgc ggcgcaccgc gccagacgtc
    59701 ccgcccacgt catcaacctg accctgctgc cggtcagcga ggccgatctg gactaccttt
    59761 acggcgccct cggccaccgg gaggtgtcca tcctgtcccg cggctacggc aactgccgag
    59821 tcaccagcac gcgcctcgcc cacgtgtggt gggtgcagta cttcaacagc atggataccc
    59881 tgatcctcaa caccctggag gtggtggaca tgcccgacgt ggtgctcgcc gcggaagagg
    59941 acttccgcga cagcatcgaa cgcctgcggg aatacgtcgc cctgctggcg gaggcctgag
    60001 ccatgttcga aggcagcttc ctcggcgacg ccgccaggct ggcggatacg gaccgcctcg
    60061 aatgtggcat ctgctggacg gtctacgacc cagcggaggg cgatccccac tggggcatcc
    60121 cgcctggcac acccttcgcg cagctccccg agcactggcg ctgtcccgtc tgcgacgcgc
    60181 cccagcacaa gttcatggtg ctgcgtgact gaggcgccgc ggcccgcgac ggtcccggtg
    60241 gccgcggcgg agctgctcgc ccgcatcgaa gcccattacc gccgcgtggc tgccaccgcc
    60301 atggcggacg tgcccctgtg caacccgcgc ctcgccgtgc ggggggtggg catcgaggac
    60361 tgggacgggc agcggatcgt cgccctggtg acgccctggg cgatcaacct gctgcgcctg
    60421 cccggcagcg cgccgctgcc ttcccgaggg cccctcgcca agcacgtctg gcgcctgccc
    60481 agcggcgact acgcttttgt cgaagccggt gacgtgggct tcggcccctt ccagagctgc
    60541 tccctgttct cgccggtgct ggagttcgac agtcaggccg ccgcctgtga gaccgccctg
    60601 gccgcgctgc tggcgttgct gcgtccgccc ctggcggtac cggaagccgt cgcggtcgcc
    60661 gacgcacgcc cgagcggcac gcctgccccg tcgcggggcg ttccgccgcc cgcgcccgcg
    60721 gaagtcccgt cccgccgtcg ctggctgctg gggcgcggcg catgagcacc ctgtccctcg
    60781 ccggcacgct caaggtaacg gcccgctggg cctttggcgg gctccacgac ctgcaggtgg
    60841 acctcaagcg gccgcctgtc gcccgcgcct tggtggggct caccccgggc gaggcgctgg
    60901 cccgtctgcc ggtgtatttc aacctgtgca gccaggccca gaccgaagcc gggcgcctgg
    60961 ccctggccgc ggcgggcatg cccctcggca cgccggtcga gagctggcgg ctgtgggcgg
    61021 aatgcctgca cgaacacctg tggcgtctgc tcctggactg gccgcgggtc atgggcgcgg
    61081 tgcccgagac ggccgccttt gccgcctggc gcgcgtgccg cgcccagggg cgcgaggcgc
    61141 tcgccgacgc cacccgcgag ctgctcgagc accgggtgct ggccggcccc ctgggcggcc
    61201 tgctggcgcg tctggaggtg cggctccacg tggggcaggg cgacgatccg gtcggcgccg
    61261 ggctggaggc ccgcgtgcag gcgatccgtg acgcctgcgc cgcgctggcc gccaaccggg
    61321 actacccccg tggtagcctt ggcggtgagg ccggcgggcg ggcggaaggc tgggtgcaca
    61381 ccgcccgcgg cgcgctgcac caccggctca ggctggcggg cggccgggtg gccgactggg
    61441 agatcgacgc gccgactgac cgcaacttcg ccgacgccat cgccctgcgg cgacgcctgc
    61501 cggagcacct gccggatccc cgggaggcga aggcggcgtt ggagcgggcc gtgctgctgc
    61561 tcgacccctg cgtgccctac ggggtggaga tcgaccatgc atgaaatgtc cctcgcggag
    61621 ggcatccgcc agatcgtcga ggacgctggc cgcagccagg gcttcggccg ggtccgtgcg
    61681 gtggtgctgg agatcggcca gctcgcggcg gtcgaagtgg acagcctgcg cttctgcttc
    61741 gacgtggtga tgaaggattc catcgccgcg ggcgcgacgc tgacgatcga gaccctgccg
    61801 ggcaccggct ggtgcatgca gtgcgcccgg agtgtgccgg tgacggccct ctacgaggcc
    61861 tgtccgtgct gcggcagcta ccaggtgcag cccaccggcg gcaccgagat gcgggtcaag
    61921 gaactggaaa tcgactgaag atcatgtgta cgacatgcgg ctgcggggcc ggagaaaccc
    61981 gtatcgaagg cgacgcgctc cacgaacacg tgctggcgga cggcaccgtg ctacgccaca
    62041 gtcacgccgc cgaccatccc tggcggccgc gcaaggtccg cggcgtgccg cccgaggcca
    62101 gcccttcggc ggccgacgat ccggcggtgg ccaccgatga cggcgccctg cactacggcc
    62161 acggaccggc cggcgcccac gcgcccggcc tcagccaggg ccagatggtg cgcatcgagc
    62221 aggacatcct cggcaagaac gacggcctcg ccgccggcaa ccgggcctgg ctggcggcgc
    62281 ggggcatcct ggccctgaac ctggtgtcca gccccggctc cggcaagacc accctgctgg
    62341 tgcgcaccat cgaggcgctc cagcggcgcg tgccggtgtg cgtcgtggaa ggcgaccagc
    62401 agaccagctt cgacgccgag cgcatccgcg ccaccggcgc gccggccctg cagatcaaca
    62461 ccggcaaggg ctgtcacctg gacgctcaca tggtcggccg cgcgctcgag cgcctcgcgc
    62521 cccccgccga cagcgtgctg ctcatcgaga acgtgggcaa tctggtgtgt ccggcagcct
    62581 tcgacctggg cgaggcggcc aaggtggtgg tgctgtccgt caccgaaggg gaggacaagc
    62641 ccctcaagta cccggacatg tttgccgccg cccgcctcat gctcctcaac aagaccgacc
    62701 tgctgcccta cctgaacttc cgcgtggacg acgccatcgc ctacgcccgc cgggtcaatc
    62761 cggccctgga ggtgatccag ctgtcggcca ccagcggcgc gggcttcgac gcctggctgg
    62821 cgtggatcga cgcgcgtcgc gcagaggcgt ccgtgcgggt gcgggacgtc gccgcgggcg
    62881 gggagggctg agagcatgct gcgcaagatc gatccggagc gggttgcccg ccgcatcgtc
    62941 gtccgcggtg tagtgcaggg cgtcggcttc cggcccttcg tgttccgcat cgcgcggcgc
    63001 catgggctcg acggctgggt gcgcaacgac ggcaccggcg tggaaatccg cgtgcagggc
    63061 gccgccgtac gggtcgagcg cttcatccac gcccttgctg agcaggcgcc gcccaccgcc
    63121 cgcgtgagcc tcatcgaacc cgaggtgatc gcgccggacc cgcatcgcag cggtttctcc
    63181 atcctgccca gcgccggcgg acagatcgcc accaccatcg gacccgacct ggccgtgtgc
    63241 ggcgactgcc tcgaagagct cttcgacccg cagaaccgcc gctaccgcta ccccttcatc
    63301 aactgcaccc actgcggacc gcgctacacc ctcacccggc gtctgcccta cgaccgggcc
    63361 accaccagca tggccggctt cgccctgtgt cccgaatgcg gcagcgaata cgccgacccg
    63421 gccaaccgcc gcttccacgc cgaacccaac gcctgcccgg cctgcggccc gcgcctgtcc
    63481 ctgcgggagg tgcacgacgt gccggccctg gtccgcgacc ccatcagcgc caccggccag
    63541 cgcctgcgcc tgggcgagat cgtcgccgtc aagggcctgg ggggcttcca cctggcctgc
    63601 gacgcggcca atcccttcgc cgtcgccacc ctgcgcgacc gcaagcggcg gcccgcccag
    63661 ccttttgccc tgatggtcgc caatgtggcc agcgcgcgcc gcctggcgcg ggtgtcggac
    63721 gaggaggcgc ggctgctgga gagtcccgag cggcccgtcg tgctgctcgc caaggcggcg
    63781 gatttcgatc gccagttcac cggcatcgcg cccggcctgg acgaagtggg gctgatgctg
    63841 cccgccacgc cgatccagtt cctgctcttc cacgaagccg ccggccggcc cgatggcact
    63901 gcctggctgc aggcggccca ggatctgacc ctggtgatga ccagtgccaa tccccacggc
    63961 gaacccctgg tgcgggagaa cgaggaggcc ttcgagcggc tcgagccggt ggccgacgag
    64021 gtgctgttcc acgaccggga gatcgtcgcc cgctgcgacg attccgtgct gcgggtccgt
    64081 gccgacctgc ccggccacgt ccagttcctg cgccgtgccc gcgggtacac gccgctcgcc
    64141 tggcccctcg gccgcagcgg cccggcggtg ctggccttcg gcgcccacct caaggccacc
    64201 gtgtgcctca cccgcggcgc cgaggccttc ctgtcgcccc acgtcggcgg tcttgaaaac
    64261 ccggccgcct gtgcggtgct ggacgagacg gccgaacgtc tgcaggccct gctcgccacc
    64321 gtgcccgacg tggtcgccca cgacctccat cccgactacc actccacccg cgctgccctg
    64381 gccctggccg agcgtctcgg cgtgcctgcc gtcgccgtgc agcaccacca cgcccacatc
    64441 gccgcggtgt gtgccgagca cggcatcgac ggtccggtgc tgggcttcgc cgccgacggt
    64501 agcggcctgg gcagcgacgg cctgccctgg gggggcgagc tgctgcgggt ggacggcgcc
    64561 gacttcacgc gcctgggtca cctcgccccg ctggccctgc ccggcggcga caaggctgcg
    64621 cgtgagccct ggcgcgtcgc ctgcggcgtg ctccacgccc tgggcctgga cgaactcgcc
    64681 gcccggcggt ttgccgacca ggacgtgacg cgtctgctcg atctcctcgc ccgcggcctg
    64741 cgctgtccgc ccaccacgtc cctgggccgc tggttcgacg ccgccgccgg cctgctgggg
    64801 gtgcgcagcc tgtccctcta cgaaggccat gccgcgatgg cgctggaaac gcaggcgcgc
    64861 cgttacggcg cggcgctgcc ccaccccggc ggctggcaca tggaggagcg gcccggtgac
    64921 ggcagcgtgc tgagcctcta ccccctgttc ccgcagctcg ccgacgagac cgcgcccgcc
    64981 cgcggtgcgg cggtcttcca cgccaccctg gtggatggcc tggtacgctg gatcggcgat
    65041 gccgcccggg cccagggcct gcgccgggtc gccctggggg gcggctgcct gctcaaccgc
    65101 ctgctcgccg aaggcctgcg caagggcctc ctggcccgcg ggctggaggt cttcgaggcg
    65161 ggagaattcc ccaccggcga cggcggtatc gccctcggcc aggcctgggt ggcacgtctc
    65221 gccgtgcggg acggacggat cggatgcggt ccgcgatgat tcctgaggga gtgtgaagca
    65281 tgtgtctggc catcccgacc cgcgtggtcg aactcctgcc cgacgcccag gccctggtgg
    65341 acctgggcgg cgtgcgcaag accgtctccc ttgaactggt ggacgacgtc gcgccgggcg
    65401 attacgtgat cgtccacgtg ggctacgccc tgacccgcct cgacgcggac gaagccgagc
    65461 gcaccctggc cctgatggcc gaggccggcc tcgacgtgac ggggggcggc gcatgaagta
    65521 cgtggacgag ttccgcgacg gcgccgtggc ccgcggcctg gccgccgcca tcgcccgcgc
    65581 cgtgcagccc gatcggcgct atgccttcat ggagttctgc ggcgggcaca cccacgccat
    65641 ctcccgctac ggcgtggccg atctgctgcc accagcggtg cgcatggtgc atggtcccgg
    65701 ctgtccggtg tgcgtgctgc ccatcgggcg catcgaccag gccatccgcc tcgccctcga
    65761 cgccgacgtg accctgtgca cctacgccga caccctgcgt gtgccggcct ccgacggcct
    65821 ctccctgatg cgcgcgcggg cccgcggagc ggacattcgc atgatctatt ccgccgccga
    65881 cgccctggcc ctggcccagc gggaacccca gcgccaggtg gtgttcttcg ccatcggttt
    65941 cgagaccacc acgccgccca ccgccctggt ggtgcgccag gcggcggccc tcgggctcac
    66001 caacttcagc gtgctgtgct gccatgtgct cacgcccgcc gccatcaccg ccatcctcga
    66061 atcgcccgag gtgcgcaccc tgcacacggt ggccctggac ggcttcatcg gccccgccca
    66121 tgtgagcacg gtgatcggca gccggcccta tgcccagttc gccgccgact accgcaagcc
    66181 ggtggtcatc gccggcttcg agcccctgga cgtgatgcag gcca
//
LOCUS       DAOQMM010000007        64773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_69_length_64773_cov_43.807411, whole genome shotgun sequence.
ACCESSION   DAOQMM010000007 DAOQMM010000000
VERSION     DAOQMM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 64773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64773
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_64773_cov_43.807411"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PLH85_02205"
     CDS             <1..1249
                     /locus_tag="PLH85_02205"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNB63398.1"
                     /translation="ADDGHGGTVSDSFALSLGDVNDAPTQVSSIPDQAFSGAGAWSFT
                     LPAGVFHDADNDTLSLSARQADGSALPGWLSFDAATGSFTGVPPFGRTPVDIVVVARD
                     GHSGEVTAGFHFESNVPPPPLAPEPQAPTVLFVPPPPPRVIPDAPPTAPPSPFLPPGS
                     PVALVNPSFDSPPPVSAPLPAVPVPPERPGSDRAPVLAAPPDALPPGSSPLVSVGPLP
                     DGLPAASTRGGLVVTRPDQGAFDVVPARANTITLSAGTFVETVPGVSVSVSAMLDNGQ
                     ALPPWISFNPGTGTLLVNPPPGLKRDVTIRIVARDQNGQEAVTFVRVRVGSQRTTSSL
                     TPAADALREVRPQVMPLDDLTRPDGSAALPARPVADVARPAGHTGRDSLASQLQRAGR
                     GPGVSGRLQAIAQAAEAARRDGA"
     gene            1270..2631
                     /locus_tag="PLH85_02210"
     CDS             1270..2631
                     /locus_tag="PLH85_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB63399.1"
                     /translation="MNPLLVLLELGQRARAAGTLPELGFLLVNDTRQLMPYRQALLWF
                     EGEGVRAVSGLLAPEANTPFIQWTHRLMGHLSSAPVTPGPVHTVHAAQLPDDLAAEWS
                     EWLSPHVYWLRLPADPGRSDARAGGVLFVCEAPWDDTLHALVGEWIATWHHAWNARLR
                     PTPWSALVWRQALKKAWHTDGGHPWWKRTPVRIALLIALLLVWPVRLTILAPGELVPV
                     DPAVIRAPLDGVVAQVHVRPNQAVKAGDLLFSFDEAPIATRLEVARQALTTAEAEYRQ
                     LAQLALSDPRSKAQLATLLGKVAEKQAEADFLAGQSARAKVTAPQDGVALLDDPAELI
                     GKPVQTGERIMRVANPRSREIEAWIPIGDAIPLDDAAPVDLFLSASPLSSVSGHLRYL
                     AHDALPRPDGSYAYRLRATLDDATDHRIGMKGTAKVQGHRVPVLYWVMRRPIASIRQF
                     LAW"
     gene            2640..4742
                     /locus_tag="PLH85_02215"
     CDS             2640..4742
                     /locus_tag="PLH85_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB63400.1"
                     /translation="MTALGLPPLRQELALLPGPTLPDGQPSWTLHDPARNLFFQLDWP
                     TVAMLQRWEMGPDALLDDIARATTLHLDASHLQTVLAFLKQNQLLATLPGQAAAMAEQ
                     LARRKGGIGQWLLHNYLFFRIPLIRPDAWLTRLAPRIAGLYTRRFVWLTLLAGLFGLL
                     GVMREWDTYRNTLVEMVSWSGALAYGITLIGVKVLHELGHGFTAKRFGCRIPTMGVAF
                     LVMWPVAYTDTNEVWRLTSRRQRLQVAAAGIVTELAIALWATLLWVLLPDGPVRMGAF
                     LLSSSTWISTVLINASPFMRFDGYFLLADALQIPNLHTRAFALARWDLRERLFHLGAP
                     RPEPFPAARHASLILFAWATWIYRLVLFLGIAFLVYHFFIKAVGILLFLVEITWFVAR
                     PLASELAVWRELWPILRTRPATWRSLLLAASGLALILLPWPARVVQSAVLYPISEQVV
                     YAPRNAQLVARHAAPGQVVGRDAPLFELASPELDHRERQNAARREQLAWQSGASAFDA
                     EQRKDWQILNEQLGISQAESRTVQADAGQYHPRATMDGIVRDTDPDLHPGDWLTPREV
                     LARVTGRGILRAVVYVEDEALGRLSVGDRAVFIADGHDGPDLRMHVVEIDSDATHVLG
                     EAELASLFGGTVSVREKHGVLYPERAVYRVVLQSDAELPPPQHRWRGTLAIACAWEAP
                     GLRYVRQAAAVLWRELGF"
     gene            complement(4936..5217)
                     /locus_tag="PLH85_02220"
     CDS             complement(4936..5217)
                     /locus_tag="PLH85_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB63401.1"
                     /translation="MVKSQLVARLHAKFPDLDEETVKEAVTVILNGMARHLSDGDRVE
                     IRDFGVFALHAREARISRNPRTGEPVQVEAKSVVRFKPGKELKSRVEQG"
     gene            complement(5280..5816)
                     /locus_tag="PLH85_02225"
     CDS             complement(5280..5816)
                     /locus_tag="PLH85_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63402.1"
                     /translation="MRRRGIAGLCALVLALVGWGARAAGVTEALIRTSDSSWWPLLME
                     LSRQGEALGVREQVSPAEQSVSFELETEAAFETSQATLAEPFARFMDAVAAAVGDHPG
                     AHVTIIGFGDRPATRYNPVLLAERVRAVQRTLVQGGVGMADTQVGVRTGSEFDGDGGL
                     RNKAAAGAVIVVRIGSRR"
     gene            complement(5831..7060)
                     /locus_tag="PLH85_02230"
     CDS             complement(5831..7060)
                     /locus_tag="PLH85_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB63403.1"
                     /translation="MDLHSAPFLLLGILLLGGLVGGEAVRRLFNLPRTTGYVLTGIAA
                     GPSGLGWVAPDVLQSAQFFVYLAIGVILFELGHRLPMPGRGLDRRLLGLSLADINGTF
                     LAVGAVMLALGFGIGGALFAAAVAVSTSPAITIATTSDVGADGPATERLFALVAFNNC
                     AAFVALAVALPILPLEGLTASLAGHAPPVAYVAGSLALGTALAAMACQGARWLGPQPE
                     HQHLLLLGLICIGAGLPLGVQMSPLLTLLSLGVATRWMDVRGDVVGLRISSDARVFLV
                     ITFVLAGAALEIGLIVAHWREVAAFALARIAGRMLALRAAAGPLGVDAREAHWLGIGL
                     LPKSSIALVLLGNAGPLSGDVGERLANVLLGAIALMQLVGPICTQMCSRGLGEARRLK
                     GADPVTWMLARLPRRNG"
     gene            7236..7760
                     /locus_tag="PLH85_02235"
     CDS             7236..7760
                     /locus_tag="PLH85_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB63404.1"
                     /translation="MKTAIPSPARPATEPAPDALSRPLTLAVLKQFRLIYGSVRQHFR
                     EVEQLCGISGAKVWLLHEISGTPGIGVSELARRLSIHQSTCSQLVDKLEARGLVTKQR
                     LPSDQRRVGLHVTDTAADLLRRIPGPAEGLLPDALQGLSEDSLHVLHDHLAQLIDQLA
                     RLDEPSADKPLADL"
     gene            7823..8344
                     /gene="pal"
                     /locus_tag="PLH85_02240"
     CDS             7823..8344
                     /gene="pal"
                     /locus_tag="PLH85_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNB63405.1"
                     /translation="MKRTLLPVLLASLWLGACSTTPKPTVTAADAASDGVRSAPATAG
                     AVSATDVEARKLAEQREALAKKSVYFAFDDFTVAEKYRDVIRQQADFTLAHGADTIAV
                     EGNADERGSREYNLALGQKRAEAVRKSLVLLGVPDARLEAVSFGAEKPRESCHEEHCW
                     AENRRVDFNHRDK"
     gene            complement(8635..9075)
                     /locus_tag="PLH85_02245"
     CDS             complement(8635..9075)
                     /locus_tag="PLH85_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNB63406.1"
                     /translation="MGTNLGGGLLGPVFALAGWTGCVLLLIAFRRLSAGFRGDVHVRA
                     FALGEDASEPLPARLANRNYMNLLELPVLFYVACLMAMMIGRGTPAMVGLAWLYVALR
                     MAHSLVHVTYNHVMHRFAVFAVSNGVLVLMWVLLGLQVFGRAGA"
     gene            9418..10230
                     /locus_tag="PLH85_02250"
     CDS             9418..10230
                     /locus_tag="PLH85_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63407.1"
                     /translation="MTPTTTELEQLLMQRSLTDEQLQAAAEAAADFRILPAATVIKIG
                     GQSVIDRGRAAVYPLVDEIVVARKTHKLLIGTGAGTRARHLYSIAAGLNLPAGLLSQL
                     GASVADQNAAMLGQLMAQHGISAVSGAGLAEVPLYISEVNAVIFSGMPPYTLWMRPAA
                     EGVIPPYRTDAGCFLMAEQFGCKAMIYVKDENGLYTANPKTTKNPTFIPRISVDEMKA
                     KGLHDSILEFPVLDLLKAARHVREVQVINGLVPGNLTRALAGEHVGTIITAR"
     gene            10241..11074
                     /locus_tag="PLH85_02255"
     CDS             10241..11074
                     /locus_tag="PLH85_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63408.1"
                     /translation="MAETPNTIKHVASPLARQTLLDSSLTRPVAGVRPIRLLPWLQVV
                     KIGGRAIMDRGADAILPLVAELRALLPEHRMLILTGAGIRARHLYGVGLDLGLPVGSL
                     APLAASEAGQNGHILAAMLAPDGVSYVEHPTIANQLSIHLAAARAVVGSAFPPYHHHE
                     FPTSRIPPHRADTGAFLIADALGAAGLTIVEDVDGVHDADPNGPDGATARLIRETTSA
                     ELADRAGPLPFDRALVDVMATARHIEKVQVVNGLKPGSLTAALRGEHVGTLIHTGARR
                     A"
     gene            11461..11880
                     /locus_tag="PLH85_02260"
     CDS             11461..11880
                     /locus_tag="PLH85_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63409.1"
                     /translation="MKKRYWALLALLVVIALDAWLRMPDARSRQLTAALEAQASPEMK
                     AYPYHFHVMKFQGDTAWLSTPRNVDVPAARAIAAMFPEVDTHNPDNPAFIAAQQRLGE
                     VQGQARAIVLSQSGVAQVRWELDRDWLTAHAIDVPTH"
     gene            12031..13350
                     /locus_tag="PLH85_02265"
     CDS             12031..13350
                     /locus_tag="PLH85_02265"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB63410.1"
                     /translation="MAFLSRLMGLFGGRKADPAPAPAAPPPARNPRAAPTPPVPAEPR
                     IFVGWTEMIDAKARIGAYLLAPNGLQAGATVSGPQLLEALERERVARFAERRPVLIPI
                     TPTQWAAADFRRIAAPLTVFLLRELPTHAAARQALLAELRSHNLRVALVAADWTSDDP
                     ACAPDMLLVDLRSAPLARIEAQVQQIRRTHPRMVLAAENLDSWSEHRLCQSLGFAYSL
                     GAFTTTADEPDETRQISGSRLVVLELLNLLRTEAPPAQIVATAKRDPAIVLRLLALAN
                     SPAYGLAKQVSNLEEMMMVLGRDALYRWLAVALFRLGHDDGRDQTLLVVALGRAGFLE
                     SLVLESERATRDELFLVGLLSVIDSLLGQPMDKVLDRMTLPPAVTAALLRRDGPYAPY
                     LFLVTALERCRLDQAAMVAEALKIDTAHLIDCYSQAMEWATDELMRD"
     gene            complement(13363..14619)
                     /locus_tag="PLH85_02270"
     CDS             complement(13363..14619)
                     /locus_tag="PLH85_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB63411.1"
                     /translation="MSSVEPSSRLPGPLRALAHRNFRLYFFGQAVSILGSWIQQVAMA
                     WLVYRLTGSSALLGVTAFAALIPQLLVGPLAGAWIDRHDKRRLLLGVQVLLVAQALIL
                     AGLTWAEAVGPTMLVILAAGVGVLNSFDTPLRQSLIGAFVARREDLPNALALNAMLFN
                     SGRFVGPPIAGLLLGWTSEAACFALNACSFVALFVAVWRVKDSATPRAAGSMGHIFRE
                     GVRYAWETWLVRSLIVILVALNLTASSYAVLLPVFARDVFGGDARTLGWLWGAAGCGA
                     FGSTVFLATRRSVPGLVKAVCAGTAISAAALLVFGLTTRFGVALAAMVGLGFGISVCN
                     VGINMLLQSIAPDRLRGRVVSFFTSARFGFDALGGLLAGVIAAALGAGPTLVGEGVVL
                     VLFVLHLAGRARRLADQVAEHAASRP"
     gene            complement(14912..15319)
                     /locus_tag="PLH85_02275"
     CDS             complement(14912..15319)
                     /locus_tag="PLH85_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB63412.1"
                     /translation="MKITVETTIRAPLAAVWRAYTTPEDIVLWNAASDDWHTTRASVD
                     LRVGGTFSSRMEAKDGSFGFDFAGTYTQVVPERLLEYAFGDRRARVEFSEDATGVRVK
                     ISFDAENEFPAEQQRSGWQAILDNFKRLLEGRQ"
     gene            complement(15538..16257)
                     /locus_tag="PLH85_02280"
     CDS             complement(15538..16257)
                     /locus_tag="PLH85_02280"
                     /inference="COORDINATES: protein motif:HMM:NF037310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63413.1"
                     /translation="MKLTRLPRPRGEPALAVVSHDLSLCQPVEGIARSLDAALADWAT
                     VAPQLAEVGHALEFGAARHAWPFSEQMAQAWPVAGIERMCAPPDGDAAAADLTLVVWT
                     GALPRRATPDAIRSALRLLTFGVGVPGQGGTLAPVAVSPDELEGVWTADGRLQVSIRC
                     RPAEGPDRVTGREHPQGCFEVINALGEASRAGPQPPGVWCFGAQAPVVPVRPAGYRLE
                     IVDARGGNLCGRIPGVRTSPS"
     gene            16417..16917
                     /locus_tag="PLH85_02285"
     CDS             16417..16917
                     /locus_tag="PLH85_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNB63414.1"
                     /translation="MSDKTLLIVYHSMTDATRQLAVAVAHGAACEPASVAVRLLRAPD
                     ASPDDVLAADGYVFATPENLASMAGMMKDFFDRCYYPALDRVNGRPYASLVCAGSDGS
                     GAVRQIARIATGWRLKEIAPPLVVCTHAQTLEAILAPKVVGVDALARGEELGAAFATG
                     LAIGAF"
     gene            complement(16914..18152)
                     /locus_tag="PLH85_02290"
     CDS             complement(16914..18152)
                     /locus_tag="PLH85_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB63415.1"
                     /translation="MTQTDLRHVIQALSEALDLVGVDDVAHGKRVGLMAAECARELGL
                     TDAEVRFCFDLGMLHDIGVSSTRTHGKLVGHFDWDGAQDHCETGFALLGRFPPLQPLA
                     LPVRYHHTRWEVLTTIGLPDDVARQANLIYLVDRVDALAASWQARGELLLHTGRIRDE
                     IAGHAGHYFDPRLVDAFLRASARESFWLMQEPRAIQAFLRTRRFDGPAVPASVQELHQ
                     LAGIFACIVDAKSPFTAAHSIGVAGVARLLAAALDMDAAACERIEIAGLLHDLGKLRV
                     PDEILDKPGRLDEAERAVMRTHSFETFQILRDIAGFEDIARWAAWHHEEPNGRGYPFH
                     LQGEQLPVEARILRVADIFQAMIQDRPYRAGLPPPEVAAFMNDLAGQGRVDASIVAVL
                     LPRLDEAVDIGRGLRAACAA"
     gene            complement(18290..18724)
                     /locus_tag="PLH85_02295"
     CDS             complement(18290..18724)
                     /locus_tag="PLH85_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNB63416.1"
                     /translation="MVVLACLALLIVTTVIHYEVLRGLSLLLPTLTIRPRARLLVVIF
                     STFFAHIVEIALYALVVYALVQWGGYGTLDESSRFSLDVSLYFSAETYTSLGYGDVVP
                     GGAVRLLAGVEALNGLLLIGWSASYTYIAMERFWRDSNGQGG"
     gene            complement(18822..19742)
                     /locus_tag="PLH85_02300"
     CDS             complement(18822..19742)
                     /locus_tag="PLH85_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB63417.1"
                     /translation="MLRPVLRLLSVLLLLAVTGGARGGTLEAVPADAPEWSEEAPAVA
                     RLLERGYQAERQHLPAQAASHYCAAAREGSTEGQYRLGRLYLIGQGLARDNQLAVNLL
                     AVAAERGHERARSLLGGLPAGDRLPDCLTRGGAPEAAVAVVDEVVPQDIVDRYVRALP
                     ADKRRHAQLIQKLAPRFSVDARLALAIVRTESNFDAAARSPKNAVGLMQLIPDTAERF
                     GVRDALDPEQNIRGGLAYLRTLLAQFKGDVALTAAAYNAGEGAVTRHGGVPPYAETQE
                     YVRRILNFYRAAHHAPQGTRVAVAGGVARS"
     gene            complement(19900..20883)
                     /locus_tag="PLH85_02305"
     CDS             complement(19900..20883)
                     /locus_tag="PLH85_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63418.1"
                     /translation="MNAPTNRLMSVDDAATLIRRGAWLMIAGDETLLRRLPAGHWIGG
                     TIPYFMADEGGLASRALLFVTEIPVMAGVPEIRSYDLTSLEHLCEDAPANGFSLIIIP
                     AFSAVHSLYARKAPTFPDMFIKPVAGWVAGTHLDDAGRLSPTVVAGPRLAFDTERAVV
                     LHVPLPDSAYARIDIFNALQQGDGDTLRFPETGFSAERCQVNGVETQFAAYLKARAAD
                     TRLPLVADYGGASVNVSIKGVDETAGRVDFYAPVFDDVDYRLAAPVSFGDPTGAEAEV
                     PPLWSCNCILNYLYHGLEGRRCGSLAGPMTFGEIAYLLLNQTQVVLSIEHV"
     gene            complement(20880..21638)
                     /locus_tag="PLH85_02310"
     CDS             complement(20880..21638)
                     /locus_tag="PLH85_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63419.1"
                     /translation="MADASALWRQLLQVQEPWTVREARADEFNRCMDVWIGVEAPRSW
                     FGFGRGKPVESGPEVVWRHTGFGGWKIRVHVRPPLGGEMPRQAWAGEAGMPFTRALDR
                     QVLALLHEGVSMAAVCAFLDLPMAELWRYRYALENGQLGSGGQEAPAPAMAGVEPLRN
                     VPRTEAPVAGGVAGSRVPSADDPVWLDLLEGRRQLDVRVLSLQLLLSRTRAQVAVIAD
                     ADVRRMKLRDVHRYFVKNERMLTYELGQLVREAA"
     gene            21956..22642
                     /locus_tag="PLH85_02315"
     CDS             21956..22642
                     /locus_tag="PLH85_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNB63420.1"
                     /translation="MTRTERLFTLMQILRRHRQPVSGTDLARETGVSLRTLYRDIATL
                     QAQGAHIDGEAGVGYVLRPGFLLPPLMFSAEEIDAIVLGARWVTERADPALAAAARDA
                     LSKVAAVLPAPLRHQLDDVPLLIGPVTDSGRDAHTVAQLRTALRNERKLSIDYRDEHG
                     AASQRVIWPCALGFFERVHVLVAWCERRADFRHFRVDRIERLDLLDAGLPRRRQALLR
                     EWRAREGITG"
     gene            22760..23131
                     /locus_tag="PLH85_02320"
     CDS             22760..23131
                     /locus_tag="PLH85_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB63421.1"
                     /translation="MPHPNYILLYVTDPSASAAFYTALLACPPVEASPTFALFALDSG
                     LMLGLWARGSVEPAASLGGGGGELALTVASEAAVDACFADWRARGLTILQPPTHMDFG
                     YTCVVADPDHHRLRVFAPARP"
     gene            23241..24311
                     /locus_tag="PLH85_02325"
     CDS             23241..24311
                     /locus_tag="PLH85_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB63422.1"
                     /translation="MIPVARPRHPASRLFLSARLLAALAALSVVAPPSAAGPLRERLR
                     ERLQADDASGGPLSDLGGGEALSCSDWSRRVARVQALAGAGLRGPAADLSDVAYGAGP
                     REQLDVFVPHDRSHAGGAPLIVMVHGGGWCVGDKAMTGVTTHKVDRWTPHGFVFVSLN
                     YPMVGDGADARAQAAAVARAVAFVQRHAADWGADPARVILMGHSAGAHLVSLVNADAE
                     LRRQAGVGPLLGTVSLDAGAIDVVRQMPVVYPFLKTRYREAFGTSEAGWLAASPYHRL
                     GRDAAPWLGVCSTQRQDDPCGQARAYADKSVRLGLRAEVLPEAMNHGALNADLGLPGP
                     YTQAVERFLAGLDPAVAARLGR"
     gene            complement(24544..25020)
                     /locus_tag="PLH85_02330"
     CDS             complement(24544..25020)
                     /locus_tag="PLH85_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB63423.1"
                     /translation="MTQDDDNQNLVLGILFGVIALVIGLVIGLTTYVTGQAQTAKPAV
                     VAEEPEIAEVGEPLVKLYFDSGKAELPANAAEELAKVVAKLHEEPAKLVLISGYHDET
                     GGAAVNAEVSKARALAVKDALATAGVAADKLKLRKPAITLGGADEAEARRVEVRVQ"
     gene            complement(25219..27798)
                     /gene="clpB"
                     /locus_tag="PLH85_02335"
     CDS             complement(25219..27798)
                     /gene="clpB"
                     /locus_tag="PLH85_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNB63424.1"
                     /translation="MRFDKLTTKFQQALQEAQSLAVGNDQQFIEPQHLLMALLSQEDG
                     STASLLQRAGVNGQALRAALAKALTRLPKVEGHGGEVSVGRDLGNLLNVTDREAQKRG
                     DEFIASEMFLLALADDKGETGRLLKEHGLARKALEQAIEAVRGGQSVGSAEAESQREA
                     LKKFCVDLTERARQGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNDEVPETLKGKRVLVLDMAGLLAGAKYRGEFEERLKAVLTDIAKDEGRIILF
                     IDEIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQ
                     KVLVEEPSVESTIAILRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPEVMDKLERRKIQLEIEQLAVKKEKDEASQKRLALIEEEIAR
                     IKKEYSDLEEIWKAEKSAVQGSAHIKEEIDRLKADIVRLQREGKLEKVAELQYGTLPQ
                     LEAQLKKAEASGDAKPAHRLLRTQVGAEEIAEVVSRATGIPVSKMMQGERDKLLRMED
                     RLHQRVVGQDEAVRLVADAIRRSRAGLSDENRPYGSFLFLGPTGVGKTELCKTLAEFL
                     FDSEEHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILFDEVE
                     KAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQQMSGDDYGVIKL
                     AVMAEVKTYFRPEFINRIDEVVVFHALDEKHIASIARIQLGYLEKRLARLEMHLEVSD
                     EALAELAKAGFDPVFGARPLKRAIQEHIENPLARRILEGQFAAKDTIRVSCDNGRIAF
                     ARA"
     gene            28240..28743
                     /locus_tag="PLH85_02340"
     CDS             28240..28743
                     /locus_tag="PLH85_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB63425.1"
                     /translation="MATKQEQQFNEIQKKNLESAMRLAQLSIENSQRIMELQVGTAKT
                     LFEEGVANAKALSTAKDPAEVMTLRTAYAQSTTEKMLSCAREIAEITAKTQAEVGKMV
                     TEQLTGGGQDIFESLQKMFKGMPIGDTNALGSIQSAMDTARAAYEQMTKASTEAFQAF
                     TKMSPKK"
     gene            28898..30844
                     /locus_tag="PLH85_02345"
     CDS             28898..30844
                     /locus_tag="PLH85_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63426.1"
                     /translation="MTEKARIIDALGEPTLLLPALLGEALGASDRIKYYLSLLQAART
                     SALHPQQPAAPLMAERCAAGIDDERLDTLPARAERDHDGQIHIPDAGILCDHIVRDLD
                     ALLAPIRSAGAEAAVALSARAAALRSQPWCESCDTFDAGQFSRLTSADRDAGDSVHLL
                     VLDAHKELNRLQTRIASEDIDGAAAYDIRPADRALIAAFMRGVNASRPLKLDHPGLGT
                     TATRSGGRLVLQNDIGTTDAHVLVVHVENRRVTLTYTDVHLQRLLFFQSLFAHWAVSW
                     EDTRSRRDRGMEDGLYHVSVGTFSANNDAGLGDYLSFLGSRLVFLIDWNRARKRLRLL
                     LPKKETLDLLHWAADENVGHMAWLRSGGEQLVFDAVAFAARTPPTFGARLDDMLDPGK
                     AVGFMQSVLRACTRGFLERRPDEVVRDEIRAELATCFRSARQQIIDIVAEHAAFSIEI
                     ATGIRDCLPDLTGPDAAARIAANAARARQWEQRADERLNQARELRQQQGEWDDFYHDL
                     VARADDITDELEEAAFHLTLFPPEAPSAELLECFATLAGLAVSGAQEFLKAVETARGL
                     RRGGPREDIQDFLAAIHQILVAERQSDDTHRRFKQCLVATPGEFRSHYPWAEFARNLE
                     TACDALLHAALQLRDHIMLTVTRD"
     gene            30849..32468
                     /locus_tag="PLH85_02350"
     CDS             30849..32468
                     /locus_tag="PLH85_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNB63427.1"
                     /translation="MDLNAHLYAIGLPGPRQEGSADAMGFKAWNLARLAAIGLPVPPA
                     FVLGTPLCARHRADPAAFRPHLRTLLETQIERLEAVCGLEFGSERKPLLVSVRSGAAV
                     SMPGMMDTVLDVGLTDATLRGLLRVTGNPRLAWDSYRRLIQQFAEVVHGAPAEPFQTL
                     LHDAIAQAGVSRAQDLDFRDLSRLCRHYQDAYAQTVGQPFPQDPAQQLLDATAAVFDS
                     WMSPRAVAYRRLQGLDDGLGTAITVQRMVFGNAGGTSGAGVGFTRDPASGENRLYLDF
                     RFNAQGEDVVSGRHAAAENGRMALVLPSVLSRLQSVREILEREFGDVQEFEFTVQDGV
                     LYLLQTRAAKRTDWAALRIAVDQVTAGAWSPATALRRLDGVDLAGIVQTRIEAAGTDR
                     ELATGTPASIGVASGVIALDAAAAERFAQTGTECILVRDETRSDDLAGIAAAQGVITA
                     KGSRTAHAAVVARQLGKACLVGCNGLRVDLERRRVVFGTQSLHEGERITVDCGSGRVL
                     AGAVRAFVERPDVWLDQVARWRAIAGPALVG"
     gene            complement(32565..35063)
                     /locus_tag="PLH85_02355"
     CDS             complement(32565..35063)
                     /locus_tag="PLH85_02355"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNB63428.1"
                     /translation="MDRRNFLRNSGFLTVSVALGGLAGCGGDSGGLAVPAALGSGWKF
                     PQSVASGDPSPEGAVLWTRVVASSADDVTTASDTGDFSIRVKVTEADNSARLGTTTVL
                     AGALAVDNWIPVYAAYDGTVRNKLTGLKPATTYYYQFEAGMVLSRVGRFRTAPAKGAS
                     VDQLRLAFMTCQDWSVNHWGAFDYIVRNEDVDLIVHLGDYIYETVGASFQSGAVESRH
                     DLLVLPDGAYESGTSGPRYALTLADYRYLYKKYRTDPRLQAVHERFAFVATWDDHEFS
                     DDCWGDAETYDNGSFNSATLSGDNPHQTARRRSANRAWFEFMPADVRLDESTLSFDTI
                     RLYRDLQWGTLAHLVVTDERLYRADHVVPEATPNPATGAALGSIGSRYVVPQSTRDSF
                     EAAKMAAAGSADPLAPVSILGKTQRDWWKSTMTASPATWKLWCNEVSLLRMQLDGTNA
                     IATLFALQAVSTLANSIQGALPAVGGNAAVAGALVAAMTAGASQATAAAAALAIATAA
                     ATAGNPGTAATGAGLTAHQAAIAVAAFTAAAAANGTSAQVAAAARTIAFGYIKPDVQA
                     KGVASSFVQSSGKAAALQGFFTRFLFDCDQWDGYNAERKALMAHLKGNGIQNVVALTG
                     DLHCFDAGVVYDDFDASGGTPVMVDLVTAGISSDSLFTFYADSVGAVNADLATLIYYP
                     LSIPVSGVGTVSLRFNVFDFTMAGHVPTLDELAEQARVPLRGALAAKGVPEASLDATT
                     TAVLTALKADSSFNTDLLGLAQQLAGICANPWIKWAATDAQGYAVVTVTPGRLSCVFK
                     QVNRLVGTAAPSSTVIARTVTATVNAGSAAVTIS"
     gene            35218..36444
                     /locus_tag="PLH85_02360"
     CDS             35218..36444
                     /locus_tag="PLH85_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNB63429.1"
                     /translation="MRAPSPASAALATFRRHPLHFACLATLGLSACGGSDSLTPLPTL
                     DGNAPLVVAHRGASGYLPEETLEAYTKAIDLGADVIEPDLISTKDGVLIARHDPNLAY
                     STDVASHPEFADRKKTTQVDGETQTGWFASDFTLAEIKTLGGISTDAERPQQYNGLYK
                     IVTFQEIIDLAKAKAKETGRSIAVYPETKNPTYHRDLGLPLEDTLIGLINLAGWNSKD
                     APIYVQSFEPSSLKYLRAKGLKTKLIQLIDADDVDFKTGALTYAAPYDKPYDWFKAGD
                     TRLFSAMVTPAGLAEIKTYADGIGPWKRYIVTVKGTLDASGNVKDINGDGKINDADTT
                     SQPATTLIADAHKAGLFVHPYTFRNEQRRLASDYKGDPKNEYLAFYRLGVDGLFSDFT
                     DTAIAARTAYLKEMGR"
     gene            complement(36547..37344)
                     /gene="pstB"
                     /locus_tag="PLH85_02365"
     CDS             complement(36547..37344)
                     /gene="pstB"
                     /locus_tag="PLH85_02365"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNB63430.1"
                     /translation="MQQATTQSSQGVIKGPAQIAFRNFNFYYGEFLALKNINFEMEKH
                     RVTAFIGPSGCGKSTLLRTINRMYDLYPNQRAEGEILLNGQNLLDPKVDVTTLRARVG
                     MVFQKPTPFPMSIFDNIAFGVSLHEKLTRSEMEERVEWALTKAALWGEVKDKLNQSGM
                     SLSGGQQQRLCIARGIAVKPEVVLLDEPTSALDPISTARIEELVAELKNEFTIVIVTH
                     NMQQAARISDYTAYMYLGELIEYGETDQIFLSPKQKATEDYITGRFG"
     gene            complement(37358..38200)
                     /gene="pstA"
                     /locus_tag="PLH85_02370"
     CDS             complement(37358..38200)
                     /gene="pstA"
                     /locus_tag="PLH85_02370"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNB63431.1"
                     /translation="MDLLARRKLTNQIALGLSMGAMVFGLFWLAWILVTVLQLGLGGL
                     SLSLFTEMTPPPGAETGGLLNAIVGSLILVALGTAVGAPIGIMAGIYLAEYGRYTWLG
                     RVTRFVNDLLLSAPSIVIGLFVYAVIVATTGKFSAWSGAVALALLVLPIVIRTTENML
                     MLVPGSLREAAFALGAPKHIVISRVTLRAARSGVVTGVLLAIARIAGETAPLLFTALS
                     NQFWSVDLNEPIANLPVTIFKFAMSPFEDWQRLAWAGVLLITLGVLGLNIIARVMFRA
                     EKLH"
     gene            complement(38204..39175)
                     /gene="pstC"
                     /locus_tag="PLH85_02375"
     CDS             complement(38204..39175)
                     /gene="pstC"
                     /locus_tag="PLH85_02375"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008435.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HNB63432.1"
                     /translation="MKVSQTDVAQPARRPVSQVGAKVFGGISTTFAVLALALLAGIIV
                     ALTVASWPSIVAFGPAFLVTAEWNPPAEQFGALIPIYGTLVSSIIALVVAVPVSFGIA
                     LFLTELSPLWLRRPLGTAIELLAAVPSIVFGMWGLLVFAPIFGEYIEPALGATLGKIP
                     LLGALFSGPSLGIGLLCAGIILAIMIIPYIASVMRDVFEVTPAMLKESAYGLGCTTWE
                     VMFRVVLPYTKNGVVGGVMLGLGRALGETMAVTFVIGNTNFLSTASLFAPGNSITSAL
                     ANEFAEAERGLHTSALMELGLILFVITLIVLVLSKLLLLRLSANEGR"
     gene            complement(39283..40323)
                     /gene="pstS"
                     /locus_tag="PLH85_02380"
     CDS             complement(39283..40323)
                     /gene="pstS"
                     /locus_tag="PLH85_02380"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNB63433.1"
                     /translation="MLNAVTRSLLASAGAVLASTAFAADITGAGASFPAPVYAKWADA
                     YQKATGNKVNYQSIGSSGGIKQITAKTVDFGASDMPLSPEQLEKDGMQQFPTVIGGVV
                     PVVNIPGVQPGQLKLTGEILANIYLGKITKWNDPAIVALNTNLNLPASDIGVVRRADG
                     SGTTFIFTNYLSKVSADWKNQVKEGATVQWPLGLGGKGNEGVSAFVQRLPGAIGYVEY
                     AYAKQNKLTFASLKNKDGAFVLPDDENFKAAAAGADWSKNAFYEILTDKPGKDSWPIT
                     GATFILMHKVQDKPVQGGEALKFFEWAYHNGAKLATDLDYVPLPESTVKLIRASWAGI
                     KDASGKAVFSGK"
     gene            40654..42072
                     /locus_tag="PLH85_02385"
     CDS             40654..42072
                     /locus_tag="PLH85_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNB63434.1"
                     /translation="MRHISLLAAAVSAALLAGCGGNSSTQETPATNVIFFLGDGMGIN
                     TLTAARIYKVGEDGDLTIDTLPETAFVRTYSNNAQVTDSAPSMAAYMTGVKMNNEVIS
                     MSADTMAFDASGNNYLSGTDSTCPASGNGSAVTTLLELAKKAGWSTGAVTTTRVTHAT
                     PAVTYSHICHRDAENTIAAQMVPGGSGYNSALGDGLNVLMGGGRQFYLPTASGGKRTD
                     GRDLTAELVKQGYTYVKNKTEFDAAAAASTDKLVGLFTSSHMSYDIDRDPTVEPSLAE
                     MTTKAIDILSKNKKGFFLMVEGGRIDHALHETTAAKALQDTVAFDDAIQTALTKMKAV
                     DPTLANTLIVVTADHDHTLVLNGYAKRTGATTASNPGILGLLKNFVSGANATDSGGLP
                     FTILGFGNGENRPATRSALTDAQVSVKTYHQEAAVPMAAGSETHGGTDVFLGAIGRGA
                     DKFTGVITNTEVFGLIRSAVGL"
     gene            42093..43502
                     /locus_tag="PLH85_02390"
     CDS             42093..43502
                     /locus_tag="PLH85_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNB63435.1"
                     /translation="MKFQIKTCVAALALVGTTSAFAAGEATNVIFFLGDGMGPTTVTA
                     SRIYEYGEAGKLNMESLTRTARIKTYSNDAQTTDSAPSMAAYMTGVKMNNEVLSMSAD
                     TKAYASTATNSGYVSGADSTCPATGNGTAVTTLLELSKAAGKSVGAVTTTRITHATPA
                     ATYSHICHRDGENDIAAQAVPGSGKFNSALGSGVDVLLGGGWRFFKPNTVTGGSRTDG
                     QDLTAKLQTAGYTYVETGTAFKAVDATATSKLVGLFNRDHLNYELDRVNKSIDEPSLA
                     DMTEKAIKILQKNGKGFFLMVEGGRIDHALHGTNAKRALEDTIAFDEAIKRALSLVDL
                     KNTLVVVTADHDHTMTFNGYSHKGNPILGKVTDITQTTVKKTPVLAAAADGKPYTTLV
                     FGNGGGPRKATRDDLTSVDTTTDDYLQEVAVNLGSAGTETHGGGDVMLFSGGAGSSGF
                     KGTMVNTKVFGLVKSALGL"
     gene            43610..44275
                     /locus_tag="PLH85_02395"
     CDS             43610..44275
                     /locus_tag="PLH85_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63436.1"
                     /translation="MKKLLLAALLGALTAGARADDLALRIEHDSTVVGADGVTRNVRF
                     AERLIRRDAQSWLARILPPGAHEDAEHAAGAKGHKHMDIAAATRWVVRAPDGKLRVRL
                     VNDHDRMIVDIAPVDYANIGFDGRWSTAAQLIDPEQIRQMKPLARSAPAGTRWYESGT
                     PDARVQVLWDEKDKYPRRIESSNAAGTRRSVLNATREALPAVLPWTRLDGYALKEYSD
                     LLD"
     gene            44351..44755
                     /locus_tag="PLH85_02400"
     CDS             44351..44755
                     /locus_tag="PLH85_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB63437.1"
                     /translation="MESIRLQGVPMVFFELFSNAPDIVRVPAGKALFHEGEEGCCMFV
                     LTIGSAEVYVKNRRVELLVPGNMVGELALVTRGPRTATVLALEDSEFVSIDEDRFNYL
                     VQHTPFFALNVMRLLAERLAAASSALPAVEDV"
     gene            complement(44766..44972)
                     /locus_tag="PLH85_02405"
     CDS             complement(44766..44972)
                     /locus_tag="PLH85_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63438.1"
                     /translation="MSDPRLNHPTCAECAYFQSNGATSGTCHRYPPSFAGEHPAEHHT
                     WRFPAVTSFSWCGEFAPDGNAGPR"
     gene            45180..46079
                     /locus_tag="PLH85_02410"
     CDS             45180..46079
                     /locus_tag="PLH85_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNB63439.1"
                     /translation="MPRPRLRALRTAAFLALLSLGLSACSPRQLVVRSVADQLAGQDA
                     GSENDPELAREASAFYLKLSESVLRQDPDHLPLATAVAGGFTQYAYAFVAFDADRLEA
                     RDSRGAQALRERAARLYRRARDHALATLERHRPGLAAELATAGPERPLAKDLVPLAYW
                     GAAAWGGLVSLSKDDPDTVADLPRAIRLARLAWLADPAWGDGTLASLMGSFEAARPGG
                     SARQAEAYFDDALRLAAGRSAGAFVAKAEGVAQPAGDKAAFLDLLRQAEAVRDTPGSP
                     LTLANEVMRRRARWLAENADDLF"
     gene            46116..47138
                     /gene="dctP"
                     /locus_tag="PLH85_02415"
     CDS             46116..47138
                     /gene="dctP"
                     /locus_tag="PLH85_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNB63440.1"
                     /translation="MPLTARRHALRLLATLPALALPGLAVAAGPQMRIGTLAPKNSLY
                     HRQLLEIGEAWRAAQGEGAKFTVYPDGSQGGETDMARRMRIGQLQGALLSVVGLREIE
                     PSISALQNMPLMFRSWDEVDYVREKIRPAMEKKFLDKGFVVLTWGDAGWVRFFSKEAA
                     VRPDDYKKMKFFAWAGESEQQDIMKSLGYTPVPLETSDILPSIQTGMINAVPSTPYFA
                     LASQVYATANHMLDINWAPIVGALVVTKKAWDDMTPAAQTALRDAAAKAGAQMRQQAR
                     KEVDEAVEAMKKRGLVVHTPTPEQMKEWNDLADKLYPRIRGKMVPADTFDEVVSLLKT
                     YRAGKR"
     gene            47210..49027
                     /locus_tag="PLH85_02420"
     CDS             47210..49027
                     /locus_tag="PLH85_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNB63441.1"
                     /translation="MRRFLPDEALASAALFLMTLIPLVEMAVRPLFGKGIDNAPLLVQ
                     HLGLILAMFGALLAERGGHLTSLGNSLGSARDPRVRAAAGLFAHAAAALLCGLLGAAS
                     WAFVASELDAGRELAYGLPVWTVQAAMPLGFLLLGMRLGARCAPAGLARPLVGASLAG
                     LGFLLARHFDGSTLSFAPFAVALLAALLAGAPIFAVLGGLALGLFWSEGQPLASVPLS
                     HYQITVNPSLPALPLFTLAGLVFARSGAAGRLGTLFTACFGGGATGSAVAAAVLCSCF
                     TAFTGGSGVTILALGGLLLPLLRDAGLPERRGISLVTSASALGVLLAPSVPLIMVAII
                     ARVPINTMFLAGLLPALVMVLCLLLVGGYLRSTPRAGAPVAAPSAGVPSGVGLRAAIW
                     AARWEILAPVVAIGALASGIATPTESAALTAAYALFTQAVLHREVDATLLRRCLADCA
                     QVIGGVMLILGMALGLTNYLVDAGIPDAAVDWVQATLPNKFAFLVALNLFLFVAGALM
                     EIYAAIVVLVPLLLPVAVSYDIDPVHFGVIFLAVMEMGFLCPPAGMNIFFASAMFARP
                     IREVAVSVLPALLAIFLGALIIAWVPWLATGLPALFALH"
     gene            complement(49049..49744)
                     /locus_tag="PLH85_02425"
     CDS             complement(49049..49744)
                     /locus_tag="PLH85_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB63442.1"
                     /translation="MGTDPRPVVVVIEDEPKIRRFVCMALESADLQAFEADSVKRGLI
                     EAGTRQADLVVLDLGLPDGDGKAFIRELRTWSAIPVIVLSARTQESEKVAALDAGADD
                     YLVKPFGAAELLARVRAQLRRAAPRDGTPGVMSFGRLRADFGRRLIERDGVPVHLTPI
                     EFRLLACLASHPDTVLTHRHLALTVWGPGHAEDVHYVRVHMAQLRKKIEDDPSRPRHL
                     LTESGVGYRFCPR"
     gene            complement(49734..52457)
                     /gene="kdpD"
                     /locus_tag="PLH85_02430"
     CDS             complement(49734..52457)
                     /gene="kdpD"
                     /locus_tag="PLH85_02430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HNB63443.1"
                     /translation="MDDPRRDPQRILDRLREAEGRAARGRLKIFFGASAGVGKTYAML
                     CAAHQQRAQGVDVVVGVVESHGRRETAALLDGLDVLPPRSVAYKGRTLAEFDIDGALA
                     RQPALILVDELAHSNVEGSRHPKRWQDVEELLDAGIDVYTTVNVQHLETLNDVVGGIT
                     GVRVWETVPDHVFDAADEVVLVDLPPDELLQRLKEGKVYLPQQAERAIQNFFRKGNLI
                     ALRELALRRTADRVDSEMVRYRRDSAVSTVWATQESLLACVGPGEGGDRVVRACARLA
                     GRLEVPWHAIYVETPDLRHLSERNRQRILKNLHLAQESGAETASLAGADAAERIAAYA
                     RDHNLARIVVGRDHPRGWRPWHRSMAEQIGRQAPDLDVILVARDEARRDAAVEAPTSE
                     SWLDRLRAPWTAFAMTAALGALATVVATLLLGHFDLANIAMVFLLAVVLTAVRYGLGP
                     AVVASFLNVAAFDYFFVPPRFSFAVSDVQYVLTFAVMLASGLITANLTAGLKLQARVA
                     SRREYRIRALYEMSRDLSGALMPEQIAEIALRFSRTTFAARCTLVLADDANRLLAPLA
                     VPGGLSGVDAGICQWAFDHEAEAGCGTDTLPASPLLYVPLKAPMRIRGVLVLEPRHPG
                     RMGAPEQHRLLETFARLIAISLERVHYIQVAQATTLQMESERLRNSLLSAISHDLRTP
                     LSALVGLVDMMLLTRPAPAPALVQLAESMRDEVQRMNALVSNLLDMARLQAGTVTLNK
                     QWQPLEEVVGSALKGLESALFRHPVEVRLPDDLPLLEFDTVLIERVLSNLVENAVKYT
                     PPGTPVRIGAEGPAGGFVTIWVEDDGPGLPAGREEAIFKKFERGQSESATPGVGLGLA
                     ICRAIVAAHGGTIRAENRADGGARFLFSLPAGQPPAVPGMGGLAEDAPHGH"
     gene            complement(52509..52919)
                     /locus_tag="PLH85_02435"
     CDS             complement(52509..52919)
                     /locus_tag="PLH85_02435"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HNB63444.1"
                     /translation="MNGIREAFGMAGRAATAARGGDLGRWVGALVCLGVLGWGGAVQA
                     AGYFRSDADLQELESVLRSRVPVGTALFVKGYVLGVADAGSGTQWCLPADLTEEHLFQ
                     AVARHLPKDPPPRTSRSAAQQVSEALSSEFPCAR"
     gene            complement(52924..53511)
                     /gene="kdpC"
                     /locus_tag="PLH85_02440"
     CDS             complement(52924..53511)
                     /gene="kdpC"
                     /locus_tag="PLH85_02440"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNB63445.1"
                     /translation="MNTIVRPALMLCGLLSLLTGLVYPAAVTAVAGWVFPAQAAGSLI
                     LADGAVAGSRLIGQAFEDPRHFWGRPSATAPQPYNGAASSGSNLGPLNPALAEAVQAR
                     VQGLRAADPGNPAPVPADLVQASGSGLDPHISPAAAAYQIGRIARLRGIAPAALESLV
                     AAHTEGRQWGLFGEPRVHVLALNRALDERFPVEGR"
     gene            complement(53524..55590)
                     /gene="kdpB"
                     /locus_tag="PLH85_02445"
     CDS             complement(53524..55590)
                     /gene="kdpB"
                     /locus_tag="PLH85_02445"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNB63446.1"
                     /translation="MTRKTLSMFDPDLLRPALVDAFRKLNPAVQWRNPVMFVVYGGSL
                     LTTVLGIQALAGDGEAPAGFIFTVALWLWFTVLFANFAEALAEGRSKAQAAALRGLKQ
                     DTPAKRLTAPRYGAPWQQVSATALRRGDVCLVEAGETVPADGEVIEGVASVDESAITG
                     ESAPVIREAGGDFSALTGGTRLLSDWLVLRVTVNPGETFVDRMIAMVEGARRQKTPNE
                     IALTILLVALTLVFLVVTVTLLPFSVFSVQVAGAGAPVTLTVLVALLVCLIPTTIAGL
                     LSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFIPAEGV
                     TEQALARAAQLASLADETPEGRSIVVLAKQRFNLREHDLQALGAQFVHFSAQTRMSGV
                     DLGGTRIRKGAGEALRRHVEALGGHFPASVTAIVEETARRGSTPLVVAEDARVLGVVE
                     LKDIVKGGIRERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLAL
                     IRQHQAEGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSNPTKLIE
                     VVETGKQMLMTRGALTTFSIANDVAKYFAVVPAAFVSTYPQLEALDVMALASPASAIL
                     SAVIFNALIIIVLIPLALRGVRYRPQGAGLLLRRNLTIYGLGGLLAPFVGIKLIDMAL
                     AASGLA"
     gene            complement(55604..57394)
                     /gene="kdpA"
                     /locus_tag="PLH85_02450"
     CDS             complement(55604..57394)
                     /gene="kdpA"
                     /locus_tag="PLH85_02450"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNB63447.1"
                     /translation="MSSHAWVLLGLYLLVLLAAAVPLGRYIAAVAEGQGGRALRPGRP
                     IEALIYRLCGVRAEQEMGWRTYALAILAFNLVGALAVYALQRLQDGLPLNPQGLAAVS
                     PDSAFNTAISFATNTNWQGYAGESTMSPLTQMLGLSVQNFLSAATGMAVALALIRGFA
                     RHSAATVGNAWVDLTRMVLYVLLPLSGVVAVFLVGQGVVQDLGGDRLATTLELTHYDA
                     PRLDATGKPLIDASGRVLTEPARTQTQVLSLGPVASQEAIKMLGTNGGGYYNANSAHP
                     FENPTPLSNFVEMLLIFLIPAALCVSFGRMVGDARQGRAILATMTLLFVAMAVAGAHA
                     EAQGHPLLASLGIEGAAGNLEGKETRFGSDDSALFAVITTLASCGAVNGMHDSFTPLG
                     GLVTLWNMMLGEVVFGGVGSGLYGMLVFAVLAVFIAGLMIGRTPEYLGKKIEVHDMKM
                     VSIAILVTPVLVLAGTAVAVLVPAGGAGVGNPGAHGFSEILYAFTSAANNNGSAFAGL
                     SANTPFYNSVLGVVMGLGRFGVIVPVLALAGHLAAKKRLAPGAGTMPTHGPLFVVLLI
                     GTVVLVGALNYVPALALGPVVEHLALYAAH"
     gene            complement(57391..57483)
                     /gene="kdpF"
                     /locus_tag="PLH85_02455"
     CDS             complement(57391..57483)
                     /gene="kdpF"
                     /locus_tag="PLH85_02455"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNB63448.1"
                     /translation="MSAMLTVSAVLAAGLLVYLVVALLRPEDFS"
     gene            complement(57707..58621)
                     /gene="lepB"
                     /locus_tag="PLH85_02460"
     CDS             complement(57707..58621)
                     /gene="lepB"
                     /locus_tag="PLH85_02460"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB63449.1"
                     /translation="MFDVSPRQPLRAALMSLVLPGYGQFYNGELDKAVWLFLAFAFLC
                     VPGMALVGLYLPPAWTMPALGVSVVAALGTWGYAVVDAWRGARARPDYLPRPWQTGAA
                     YAVAFLVCNGLALPLLVDTVRSRQMESFSIPSASMEPSVLRGDILFADKRYNCPGCKQ
                     QIRRGDVAVFTYPNDRTRLYIKRIVGLPGDRVETRGAAVWVNGVALSTDPVMSGAVTE
                     TLDGRRWQVLRGGTGSTRAPEEAPPDVVLTVPPGQVFVLGDNRDATTDSRRFGPVPMA
                     DVAGRARQVWWSSGPEGVRWARLGAVVE"
     gene            complement(58641..58940)
                     /locus_tag="PLH85_02465"
     CDS             complement(58641..58940)
                     /locus_tag="PLH85_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63450.1"
                     /translation="MRTDPFDRPADAVLDPAVEALLREDFPAPPADFAARVMAALPAV
                     PQPIPPSRAPTGAPAGRGRPFARAAAWLTAVGAGLFGVAQLLSLAFAVWSVTTAG"
     gene            complement(58930..59478)
                     /locus_tag="PLH85_02470"
     CDS             complement(58930..59478)
                     /locus_tag="PLH85_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB63451.1"
                     /translation="MDTPDLLARLRSGDRQAFAALVVRYQRPLFGFLGRMGCAPALAE
                     DLAQDAFVRAWQQLDRYRPEMGALSTWLFTIARHLAINELGRSHHVRERPAAEDAPEP
                     ACPRPQPPEVLALAQQRQRLHAALRQLSLADRSLLALAYVEELAMADIARIEGCSVAA
                     AKTRLTRARQRLGRLLEPSDAY"
     gene            59839..60027
                     /locus_tag="PLH85_02475"
     CDS             59839..60027
                     /locus_tag="PLH85_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63452.1"
                     /translation="MLTQPIRSKQAVAAEREWAGQARRSSTPDQHAAPPTPSAGRGKL
                     WLLLLAVALIAPKLLHYF"
     gene            complement(60083..62293)
                     /gene="katG"
                     /locus_tag="PLH85_02480"
     CDS             complement(60083..62293)
                     /gene="katG"
                     /locus_tag="PLH85_02480"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNB63453.1"
                     /translation="MSTASQCPFHAAHGTRATTGARSNRDWWPNQLNLSILHQHGPAS
                     NPLGADFDYAAAFRQLDYAALKADLHALMTDSQDWWPADWGHYGGLFIRMAWHSAGTY
                     RTADGRGGAGSGNQRFAPLNSWPDNGNLDKARRLLWPIKQKYGNRISWADLMILAGNV
                     ALESMGFKTFGFGGGRADIWAPEEDIYWGAEKTWLATSDQPESRYSGPRDLENPLAAV
                     QMGLIYVNPEGPDGQPDPVASGRDVRETFARMAMNDEETVALVAGGHTFGKAHGAGDP
                     KWVGPEPEGAPLEAQGLGWINAFGRGTGGDTTTSGIEGAWKPNPTTWDGGYFDMLFGY
                     EWALTRSPAGAQQWVAQDVKPEHLIPDAHDPAKKHPPMMTTADLSLRFDPIYAPIARR
                     FQQDPAAFADAFARAWFKLTHRDMGPRARYLGPEVPAEDLIWQDPLPARDHPLIDAAD
                     IAGLKARVLQAGLGVAELVSTAWASASTFRGSDLRGGANGARIRLAPQKDWAVNQPPQ
                     LARVLATLEGIQRDFNAAQTDGRQVSLADLIVLAGNAAVEAAAQAGGVTVSVPFAPGR
                     VDARADQTDVESFAVLEPQADGFRNYLAQSFRVPAEALLVDKAQLLTLSAPEMTVLVG
                     GLRVLGANVGGAPHGVFTSRPGVLSNDFFVNLLDMGTVWAPTAETGETFTGRDRHSGA
                     LRWTATRVDLVFGSNSQLRAIAEVYAQADAAARFAGDFAAAWGKVMDLDRFDLK"
     gene            62619..>64773
                     /locus_tag="PLH85_02485"
     CDS             62619..>64773
                     /locus_tag="PLH85_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB63454.1"
                     /translation="MPPTRPPAATSPGKDARPRTPTPAVGSALPARRLALCACLGLGA
                     AIAHADGVQTLDVVEVTASATDLVGVAGAASEGTVTAKHLANRPLLRPAEVLEAVPGL
                     VISQHSGDGKANQYYLRGFNLDHGTDFATTLLGMPINLPTHAHGQGYSDLQFLIPELV
                     DTVRYRKGPYAADVGDFGSAGSAAIDYVRRLPQAFVELTAGSYGYRRGLVAGSPAVAD
                     GHLLAAFEWTGHDGPWAVPEDLRKLNGLLRYSDGTRSNGWSATALAYSARWNATDQVP
                     LRAVQSGLIGRYGSLDPTDGGTTQRTSLSVEASRQDADGWLRGNAYLIDYRLQLWSNF
                     TSCTLGCAPGPGDQFEQEDRRRIGGFNLAHTWYLNSLARGADFTLGVQSRVDDIGRVG
                     LYTTTARQRWGSIARDSVRQGSLGLYGEATVQWLEKLRSVAGLRADRYRFDVTADTPA
                     NSGQVDAALASPKLALILGPWARTEVYLNFGHGFHSNDARGITLTTQPDFRDPAFGTP
                     AHASTPLVRTKGSEIGVRSALLPGLHSSLALWQLDIASELVFTGDAGTTEARFPSRRR
                     GIEWANDWTPLTGLIVDADFSVSRARYTAVDAGVPGDRIPGALEQVASVGVAWDAGGP
                     WSGGARLRYVGPRPLIEDNTVRAPGSTLVNLRASHRIDPRTRISVDLLNAFNRKVSDI
                     DYYYASQLRGEAAPVDDLHTHPAEPRSLRVSLRVGF"
BASE COUNT        10336 a        24370 c        20713 g         9354 t
ORIGIN      
        1 cgccgacgat ggccacggcg gcaccgtcag cgacagcttc gccctcagcc tgggcgatgt
       61 gaacgacgcg ccgacgcagg tctcgagcat ccccgaccag gccttcagcg gtgcgggagc
      121 gtggtccttc accctgcctg ccggggtctt ccacgacgcc gacaacgaca cgctgagcct
      181 gagcgcccgc caggccgatg gcagcgcgct gccgggctgg ctcagcttcg acgccgccac
      241 cggcagtttc acgggcgtac cccccttcgg ccggaccccg gtcgatatcg tggtcgttgc
      301 ccgcgacggc cattcgggcg aagtcaccgc cggcttccat tttgaatcca acgtaccgcc
      361 cccgccgctg gcgcccgagc ctcaagcccc gacggtcctc tttgttccgc ccccgccacc
      421 gcgcgtcatc ccggatgcac cgcccaccgc gccgccctcg cccttcctgc ctcccggctc
      481 gcccgtggcg ctggtcaatc ccagctttga ctcgcctccg ccggtgtcgg caccccttcc
      541 tgcggtcccc gtgccacccg agcgccccgg aagcgaccgg gcgcccgtgc tggcagcccc
      601 gccggacgcg ctcccgcccg gatcctcgcc actggtcagc gtcgggcccc tgccggacgg
      661 tctgccggct gcgtcaacgc gcggcggact ggtggtcacc cgccccgacc agggtgcctt
      721 tgacgtggtg ccggcccgtg ccaacaccat cacgctttcg gcaggcacct tcgtcgagac
      781 ggtaccgggc gtgtcggtct ccgtcagtgc catgctggac aacggtcagg ccctgccccc
      841 atggatcagc ttcaaccccg gtaccggcac cctgctggtc aatccgccgc ccggcctgaa
      901 gcgggatgtc accatccgca tcgtggcccg cgaccagaac ggccaggaag cggtcacctt
      961 cgtccgggtg cgggtaggca gccaacgcac aacgagcagc ctgaccccgg cggccgacgc
     1021 gctgcgggag gtccgcccgc aggtcatgcc cctcgatgac ctgacccgtc ccgacggaag
     1081 cgccgcgctg cccgcacggc cggttgccga cgtggcgcgt ccggccgggc acacgggccg
     1141 cgactccctc gcgagccagc tgcaacgggc cggtcgcggc cccggcgtgt ccggacgcct
     1201 ccaggccatc gcccaggccg ccgaggccgc ccgccgtgac ggggcctgag gcacagcccc
     1261 cggagccgca tgaatccact cctcgtcctg ctcgaactcg gtcagcgtgc ccgcgccgcg
     1321 ggaacgctcc ccgagctcgg cttcctgctc gtcaacgaca cccgccagct catgccctac
     1381 cgccaggccc tcctgtggtt cgagggcgag ggggtgcggg ccgtctccgg gctgctcgcg
     1441 cccgaggcaa acaccccatt catccagtgg acgcaccgcc tgatgggtca tctgtcgtcg
     1501 gctccggtca cgcccggtcc ggtccacacc gtgcatgcgg cgcagcttcc cgacgacctg
     1561 gcggcggaat ggtctgaatg gctgtctccc cacgtgtatt ggctcaggct ccccgcggat
     1621 cccggccgca gcgacgcccg tgccggcggc gtcctgttcg tgtgcgaggc cccctgggac
     1681 gacaccctcc atgccctggt cggcgaatgg atcgccacct ggcaccacgc ctggaacgcc
     1741 cgcctgcgtc cgacgccctg gtcggcgctc gtctggcggc aggcgctgaa gaaggcctgg
     1801 cacacggacg gcggccaccc ctggtggaaa cgcacccctg tccgcatcgc gctgctcatc
     1861 gcgctgctgc tggtctggcc ggtccgcctc acgatcctcg caccgggcga actggttccg
     1921 gtggacccgg cggtgatccg ggcgcccctc gacggggtcg tggcccaggt ccacgtgcgc
     1981 cccaatcagg cggtgaaggc cggcgacctc ctgttcagtt tcgatgaagc gcccatcgcc
     2041 acccgcctgg aggtggcgcg ccaggccctg acgaccgcgg aggcggagta ccgccagctg
     2101 gcccagcttg ccctcagcga cccccgttcc aaggcgcagc tcgccaccct gctcggcaag
     2161 gtcgctgaaa aacaggccga agccgacttt ctggccggac agtcggcccg cgcgaaggtc
     2221 acggcccccc aggacggcgt cgcgctcctc gacgatccgg ccgaactgat cggcaagccg
     2281 gtgcagaccg gcgaacgcat catgcgtgtc gccaatcccc ggtcccggga aatcgaagcc
     2341 tggattccca ttggcgatgc cattcccctc gacgacgcgg cgccggtgga tctcttcctg
     2401 agcgcctcac cgctctcgtc agtcagcgga cacctccgct acctggccca cgacgccctg
     2461 ccacggccgg acggctctta cgcctatcgc ctgcgcgcca ccctcgacga cgcgacggac
     2521 caccgcattg gcatgaaagg caccgccaag gttcagggac atcgggtccc ggtgctgtac
     2581 tgggtgatgc gccggcccat cgccagcatc cgtcagttcc tggcctggta ggcgcgccca
     2641 tgaccgccct tggtctgccc ccgctgcgtc aggagctggc gctactgccc ggccccacgc
     2701 tgccggatgg tcagcccagc tggaccctcc acgaccccgc ccgcaacctg ttcttccagc
     2761 tcgactggcc cacggtggcc atgcttcagc gctgggagat ggggcccgac gccctcctcg
     2821 acgacattgc ccgggcgacg accctgcacc tggacgcctc gcacctgcag acggtgctgg
     2881 ccttcctcaa gcagaaccag ttgctggcga ccctgccggg ccaggccgcg gccatggcgg
     2941 agcagcttgc ccggcgcaag ggcggcatcg gacagtggct gctccacaac tacctgtttt
     3001 tccgcattcc actgattcga ccggacgcct ggctgacccg cctggcgccc cgcatcgccg
     3061 gcctctacac gcgccgcttc gtgtggctca ccctgctggc cggcctcttc gggctgctgg
     3121 gcgtgatgcg cgaatgggac acctaccgca ataccctggt ggagatggtc tcgtggtcag
     3181 gcgcgctggc ctacgggatc acgctgatcg gcgtcaaggt cctccacgag ctggggcacg
     3241 gcttcaccgc caaacggttc ggctgccgca tccccaccat gggcgtcgcc tttctcgtga
     3301 tgtggcccgt tgcctacacc gacacgaacg aggtgtggcg cctcacctcg cgccgtcagc
     3361 gcctgcaggt ggccgcagcg ggcatcgtca cggagctcgc catcgccctg tgggcgaccc
     3421 tgctgtgggt gctcctgccg gacggtccgg tgcggatggg cgcattcctg ctgtcgtcct
     3481 cgacgtggat ctccacggtg ctcatcaacg ccagtccctt catgcgcttc gacggctatt
     3541 tcctgctggc tgacgcgctg cagatcccca atctgcacac ccgcgccttt gcgctcgccc
     3601 gctgggatct gcgcgagcgc ctgttccacc tgggcgcgcc gcgtccggag ccgtttcctg
     3661 ccgcgcgcca cgccagcctg atcctgttcg cctgggcgac atggatctac cgcctcgtgc
     3721 tgtttctcgg cattgccttc ctcgtgtacc acttcttcat caaggcggtg gggatcctgc
     3781 tgttcctggt ggaaatcacc tggtttgtcg cccgacccct ggccagtgaa ctggccgtct
     3841 ggcgggagct ctggcccatc ctgcgcacgc gcccagcgac ctggcgcagc ctgcttctgg
     3901 cagcgtccgg actggccctg atcctgcttc cctggccggc ccgcgtcgtc cagagcgccg
     3961 tgctctaccc gatcagcgaa caggtggtct atgcgccccg caatgcccag ctcgttgccc
     4021 gccacgctgc accgggccag gtcgtcgggc gtgacgcacc cctcttcgag ctggcctccc
     4081 ccgaactgga ccaccgcgag cgccagaacg ccgcccggcg cgagcagctc gcctggcaat
     4141 cgggggcctc ggcctttgac gccgagcagc gcaaggactg gcagatcctg aacgaacagc
     4201 tgggcatcag ccaggccgag tcacggaccg tccaggccga cgcgggccag taccacccac
     4261 gcgcgacgat ggacggcatc gtgcgcgaca ccgatcccga cctgcacccg ggcgactggc
     4321 tcaccccgcg ggaggtcctg gcccgtgtga ccggtcgggg catcctgcgc gccgtggtct
     4381 atgtggaaga tgaagcgctg ggtcgcctga gcgtgggaga ccgggccgtg ttcatcgcag
     4441 acggtcacga cggccccgac ctgcgcatgc atgtcgtcga gatcgactcg gacgccaccc
     4501 acgtcctggg ggaagccgaa ctggccagcc tcttcggcgg caccgtgagc gtccgggaaa
     4561 aacacggcgt cctctacccc gaacgggcgg tctaccgggt ggtgctgcag agcgatgcgg
     4621 aacttccccc gccccagcac cggtggcgtg gaaccctggc catcgcctgc gcatgggagg
     4681 cgcccggcct gcgctacgta cgtcaggcgg cggcggtcct gtggcgggag ctgggcttct
     4741 gagcgatgcc ttccgggggc cgggcgccca caggccgtgg tcccggagca gaaggattca
     4801 accccgcgtc agccgagatg cgcggtcggc ctgatcaggc ttggcacgtc ggaacccggg
     4861 cgttgagtac cgcgacaccc ccacgcaagc cagggtcgcg ccggccgatc gcggggcgcc
     4921 gcgggccgcc gcgcgtcatc cctgctcgac gcgggacttc aactccttgc ccggcttgaa
     4981 gcgcaccacc gacttggcct ccacctgcac cggctcgccc gtacgggggt tgcggctgat
     5041 cctggcctcg cgcgcgtgga gcgcgaacac accgaaatcc cggatctcga cccgatcacc
     5101 gtccgacaga tggcgcgcca tgccattgag gatcacggtg accgcctcct tcaccgtttc
     5161 ttcgtccagg tctggaaact tggcgtgcaa gcgcgccacc agctgcgact tcaccatcca
     5221 ccgtttatcg tcctgcatga cctctctctc gtgacggctt cggcccgtca cctgcacact
     5281 caccgacggg acccgatgcg aacgacgatg accgccccgg cggccgcctt gttccgcaat
     5341 ccaccatcgc catcaaattc ggatcctgta cggaccccca cctgtgtatc ggccatgccc
     5401 acgccacctt gaaccagcgt ccgctgcacc gccctgaccc gctccgcaag cagcaccggg
     5461 ttgtagcgcg tggccgggcg atcgccgaaa ccgatgatcg tcacgtgggc acccgggtga
     5521 tcgccgaccg ccgccgccac cgcgtccatg aagcgggcga agggctccgc cagggtcgcc
     5581 tgggacgttt cgaaggccgc ttccgtttcg agctcgaagc tcaccgactg ctccgcgggc
     5641 gacacctgct cccggacgcc cagcgcctcg ccctgccggc tcagctccat cagcaagggc
     5701 caccaggacg aatccgatgt ccggatcaag gcttccgtca cgcccgccgc ccgggcaccc
     5761 cagccgacca gcgccagcac cagcgcgcac agccccgcga ttccgcgccg tcgcatgcct
     5821 gccaacgccc tcatccgttc cgcctcggga ggcgcgccag catccacgtc accggatccg
     5881 cgcctttcag gcggcgggcc tcgcccagac cgcgactgca catctgagta cagatcggcc
     5941 cgacaagttg catcagggcg atcgcgccca gcagcacatt ggccaggcgc tcgccgacat
     6001 ccccgctcaa ggggcctgca ttgcccagca gcaccagcgc aatgctcgac ttgggcagga
     6061 gcccgatgcc cagccagtgg gcctcccgcg cgtccacccc gagaggccct gcggccgccc
     6121 gcagggccag catgcgaccg gcgatccgcg ccagggcgaa tgccgccacc tcacgccagt
     6181 gggcaacgat caggccgatc tccagcgccg cacccgccag cacgaaggtg atcaccagaa
     6241 acacccgcgc gtccgacgag atgcgcagcc ccaccacgtc gccccgcacg tccatccagc
     6301 gcgtcgccac acccagactc agcagggtca gcaagggcga catctgcacc ccgaggggca
     6361 gcccggcacc gatgcagatc aacccaagca gcagcagatg ctggtgctcc ggctgcggtc
     6421 cgagccagcg cgccccctga caggccatcg ccgccagcgc cgtccccagg gccagcgaac
     6481 cggccacata ggccaccggc ggcgcatgcc ccgcgagcga cgcggtcagc ccttccagcg
     6541 gcaggatcgg caaggccacg gccaatgcca cgaaggccgc gcagttgttg aaggccacca
     6601 gcgcgaacag gcgctccgtc gccggcccgt ccgcgcccac gtcgctggtg gtggcgatgg
     6661 tgatcgccgg cgacgtggat accgccaccg ctgccgcaaa cagcgccccg ccaatgccga
     6721 agcccagagc cagcatcacc gcccccaccg ccaggaaggt tccgttgatg tcggccagac
     6781 tcaggcccag cagacgcctg tccagccccc gcccgggcat gggcaggcga tgccccagct
     6841 cgaagaggat cacgccgatg gccaggtaca caaaaaactg ggcggactgc aacacgtccg
     6901 gcgccaccca gcccaggccc gacgggcccg ccgcaatccc cgtcaataca taaccggtgg
     6961 tccgcggaag attgaagagg cgacgcaccg cctcgcctcc cacgagtcca cccagcagca
     7021 gaatgcccag caacaggaag ggcgcagaat gtagatccac cgcgtacgag gtcaaaaaag
     7081 tctgaacacg gccacgcaca ccccgtcccg ggacatgcgg gccactggca ctccaccccg
     7141 ctgcggcgca ggcgttgggc gacatccgtt caatgtgccc ggatgggctc aatcagggct
     7201 tcacaaatac attgcaacaa atataattga caccaatgaa aactgcaatt ccgtcccccg
     7261 cacggccggc gacagaaccg gccccggacg cactgtcccg gcccctcacc ctggctgtcc
     7321 tgaaacagtt ccggctgatc tacggttccg tgcgccagca tttccgggag gtcgagcaac
     7381 tctgcggcat ctccggcgcg aaggtgtggc tgctccacga gatctccggt acgccgggca
     7441 tcggtgtatc cgaactcgcc cgccgcctct ccattcacca gtccacctgc agccagctcg
     7501 tggacaagct cgaagcccgc ggcctggtga ccaagcaacg cctgcccagc gatcagcgcc
     7561 gcgtcgggct ccacgtcacc gacaccgcgg ccgacctgct ccgacggatc ccgggccccg
     7621 cggaaggcct gctgccagac gccctacagg gactcagcga agacagcctg cacgtcctgc
     7681 acgatcacct ggcacagctc atcgaccagc tcgcccggct cgacgaacct tccgccgaca
     7741 agccactggc cgacctgtga tccgcaatcg caggtcccgt cccgacatcc cttttttgtc
     7801 cgtccctcga gaaagcacct ccatgaagcg caccctcctc cccgttctcc ttgcctccct
     7861 ttggctgggc gcctgctcca ccacgccgaa gcccaccgtc accgcggccg acgcggccag
     7921 tgacggcgtg cgcagcgcac cggccaccgc tggcgccgtc tccgccaccg acgtcgaagc
     7981 ccgcaagctc gccgaacagc gtgaagccct cgccaaaaag agcgtctact tcgccttcga
     8041 cgacttcacc gtggccgaga agtatcgcga cgtgatccgc cagcaggccg acttcaccct
     8101 cgcccatggc gctgacacca tcgccgtgga aggcaatgcc gacgaacgcg gcagccgtga
     8161 atacaacctc gccctcggcc agaagcgcgc cgaagccgtg cgcaagtccc tcgtcctgct
     8221 gggcgtgccc gacgcccgcc tcgaagccgt cagcttcggc gccgaaaagc cccgcgaaag
     8281 ctgtcacgaa gaacactgct gggccgaaaa ccgccgggtg gatttcaacc atcgggacaa
     8341 gtgagccagc cgtatcgcgc ccgcttgaca ggcttcccgc gcgcggcaca tcggccgcgg
     8401 gaagcgtatc gacagcggaa aggccgggac gccaaccccc cctggggcgc cagtctgccc
     8461 gacgagacgg acctggcccg gctccgcatg gccgccccgc cctgggattt tccgtcagtc
     8521 gggcgacccg tcatcctcca gcgcgattcc gggcaccggt gcgacgctgc cagggcaggc
     8581 ctccccccgg gaaggccgcg gccacaccgc cgccccgcct gaatgccccc gggctcaggc
     8641 gccagccctg ccgaacacct gaagccccag cagtacccac atcagcacca gcaccccatt
     8701 gctgaccgca aagaccgcga agcggtgcat cacgtggttg taggtcacgt gaaccaaact
     8761 gtgcgccatc cgcagggcca catagagcca ggccagcccg accatcgccg gcgtcccgcg
     8821 gccgatcatc atggccatca ggcaggccac gtagaacagc accggcaact ccagcagatt
     8881 catgtagttg cgattggcca gacgcgcagg aaggggctcg ctggcgtcct cgccgagcgc
     8941 aaaggcgcgc acatgcacgt cgccacggaa gcccgcactc agacgccgga aagcgatcaa
     9001 cagcagcacg cagcccgtcc accccgccag tgcaaagacc ggcccgagca gccccccacc
     9061 cagattcgtc cccatcgctc ccccgtctca tacatcgacc cgagcgcatc ccggcgccgg
     9121 gctgaacgac cgccacggca cacggcggtg cacacgatgc cccgcgtgtc cgacgacggc
     9181 ccgcgtcgat gcaggcgcgg tcagacccgc gtgtcaccag gacgcgctgc acgctgccgc
     9241 ccgatcacgc tattcacgca tacggttgcc tctatacaac gctccatcct accgtccacc
     9301 cggtgacctg cccatcttgg gcgggcgcat ccgtcctgga acggggcctg gcggtccgcc
     9361 ccccaccgcc gatcgcaccc cgccacgccg gcgcttcaca acaacgaaag gatccccatg
     9421 acccccacga cgacggagct cgaacagctc ctcatgcaac gctcgctgac cgacgagcag
     9481 cttcaggcgg cggcagaagc agccgccgat ttccggatcc tgccggccgc aacggtgatc
     9541 aagatcggcg gccagagcgt catcgaccgc ggccgcgcgg ccgtctatcc gctggtggac
     9601 gagatcgtcg tcgcccgcaa gacccacaag ctgctcatcg gcactggcgc cggcacacgt
     9661 gcccgccacc tgtattccat cgcagccggc ctcaacctgc ccgccggcct gctctcgcag
     9721 ctgggcgcat cggtggccga ccagaacgcc gcgatgctgg gccagctcat ggcccagcac
     9781 ggcatctccg ccgtcagtgg cgccggactc gccgaggtgc ccctgtacat ctccgaggtc
     9841 aacgccgtga tcttcagcgg catgccgcct tacaccctgt ggatgcgtcc cgccgccgag
     9901 ggcgtcatcc cgccctaccg cactgacgcc ggctgtttcc tgatggctga acagttcggc
     9961 tgcaaggcga tgatctacgt gaaggacgag aacggcctct acaccgccaa ccccaagacc
    10021 acgaagaacc ccaccttcat ccccaggatc tccgtcgacg agatgaaggc caagggcctt
    10081 cacgattcga tcctggaatt cccggtgctg gacctgctca aggccgcccg tcacgtccgc
    10141 gaggtgcagg tcatcaacgg cctcgtcccc ggcaatctca cgcgcgcgct ggctggcgaa
    10201 cacgtgggca cgatcatcac tgcacgctga ggaatccgcc atggccgaaa cccccaacac
    10261 catcaagcac gtggcctctc ccctcgcgcg ccagaccctc ctcgacagca gcctgacccg
    10321 ccccgtggcc ggcgtgcgcc ccatccgcct gctgccctgg ctgcaggtcg tcaagatcgg
    10381 cggccgcgcc atcatggacc ggggcgccga cgccatcctg cccctcgtcg ccgagctccg
    10441 cgcgctgctg cccgaacatc gcatgctgat cctgaccggc gccggcatcc gcgcccggca
    10501 tctgtacggc gtcggcctgg acctgggcct gccggtgggc tccctggcgc ccctcgccgc
    10561 cagcgaggcg ggccagaacg gccacatcct ggccgccatg ctggccccgg acggggtctc
    10621 ctacgtggag catcccacca tcgccaacca gctctccatc cacctggccg ccgcccgcgc
    10681 cgtggtgggc agcgccttcc ccccgtatca ccaccatgaa ttccccacct cgcgcatccc
    10741 cccgcaccgg gcggataccg gcgccttcct gatcgccgac gccctcggcg ccgccggcct
    10801 gaccatcgtc gaggacgtgg acggtgtcca tgacgccgac cccaacggcc ccgacggcgc
    10861 cacagcccgg ctgatccgcg aaacaacgtc cgccgaactg gccgaccggg ccggcccgct
    10921 gcccttcgac cgggccctgg tggacgtgat ggccaccgcc cgccacatcg aaaaggtgca
    10981 ggtggtgaac ggtctcaagc cgggcagcct caccgcggcc ctgcggggcg aacacgtggg
    11041 cacgctcatc catactggcg cccgccgggc ctgacgccca caccccgggt cgtctagacc
    11101 cggaaaattc ctcactgacg ccatgggccg aaccacccgg cccgctcgca atgttctcgt
    11161 gagtgcgtcg ccccacggcc ccgtctccca cacacccgtc cagatcgaac tgctgcgccc
    11221 agaggagcac ccacgcaaca cgccggagcg cacccccgcc gtcgcgccat cgctctcatt
    11281 gaatgccgag cgcacgccca ggcgcccgcg gccgcccgac ggacgccgcg atgggccttt
    11341 aacccggatc gcgcccacac aacgggcatc ccctcgccgg accgatgccc gcaatcaatt
    11401 aacatttgct aatataccaa ttcctatccg acgcaccatc ggatgctccg catcctcctc
    11461 atgaaaaaac gctactgggc cctccttgcc ctgctcgtcg tcatcgccct cgatgcctgg
    11521 ctgcgcatgc ccgacgcgcg ctcccgccag ctcactgctg cactcgaagc gcaggccagc
    11581 ccggagatga aggcctaccc ctatcacttc cacgtgatga agttccaggg cgacacggcc
    11641 tggctgagta cgccgcgcaa cgtggacgtc ccggccgcca gggccatcgc ggcgatgttt
    11701 cccgaggtcg acacccacaa cccggacaac ccggccttca tcgccgccca gcagcgcctg
    11761 ggcgaggtgc agggtcaggc gcgggcgatc gtcctgtccc agtccggcgt tgcccaggtc
    11821 cgctgggaac tggaccggga ctggctgacc gcccacgcca tcgacgtacc gacgcactga
    11881 gccccatccc cggggcggcc ccgccaccat tcagaaccgg tcaatccggt cgataaagga
    11941 gtcctcctcc gccggcctga agggctgtcg ccccacctgc gacctcccgc cgccgtccgg
    12001 acgctcccgt atcgaccgaa ggatggttcc gtggcatttc tttcccgact gatgggcctt
    12061 tttggggggc gcaaggccga cccggcgcct gcgcccgcgg ccccgccacc cgcccgcaac
    12121 ccgcgtgccg caccgacacc gccggtgccc gccgagccgc gcatcttcgt cggctggacc
    12181 gagatgatcg acgccaaggc gcgcatcggc gcctacctgc tcgcgcccaa cgggctgcag
    12241 gccggtgcga cggtctccgg cccgcagttg ctggaggccc tggagcggga gcgcgtcgcg
    12301 cgctttgccg agcgccggcc ggtgctcatc ccgatcacgc cgacccagtg ggcggctgcg
    12361 gacttccggc gcattgccgc ccccctcacc gtcttcctgc tgcgcgagtt gcccacccac
    12421 gcggcggcgc ggcaggccct gctggcggag ctgcgcagcc ataacctgcg cgtggccctg
    12481 gtggcggcgg actggacgtc cgacgatcct gcctgcgcac cggacatgct cctggtggat
    12541 ttacgctcgg cccccctggc gcgcatcgag gcccaggtgc agcagatccg ccgcacccac
    12601 ccccgcatgg tgctggcggc ggagaacctg gactcctggt ccgagcaccg cctgtgccag
    12661 tccctgggtt tcgcctacag cctgggtgcc ttcaccacca ctgccgacga gccggatgag
    12721 acgcggcaga tctccggcag ccgcctggtg gtgctggagc tcctcaacct gctgcgcacc
    12781 gaagccccgc cggctcagat cgtcgccacc gccaagcgcg atccggccat cgtgctgcgc
    12841 ctcctggccc tggccaattc gcccgcctac gggctggcca agcaggtcag caacctggaa
    12901 gagatgatga tggtgctggg tcgcgacgcc ctctaccgct ggctggccgt cgccctgttc
    12961 cgcctcggcc acgacgacgg acgggaccag accctgctcg tggtggccct gggccgggcc
    13021 ggcttcctgg aatccctggt gctggaatcg gagcgcgcca cccgcgacga gctcttcctg
    13081 gtgggcctgc tgtcggtcat cgacagcctg ctcggtcagc ccatggacaa ggtgctggac
    13141 cgcatgaccc tgccccccgc cgtgacggcc gccctgctgc gccgggacgg gccctacgcg
    13201 ccctatctgt tcctcgtcac cgccctggaa cgttgccgcc tcgatcaggc cgcgatggtg
    13261 gccgaggccc tcaagatcga caccgcccac ctcatcgact gctacagtca ggccatggaa
    13321 tgggccaccg acgagctgat gcgcgactga tgatcccccg cctcaggggc gggacgcagc
    13381 atgttccgcc acctgatccg ccaggcgccg ggcacggccg gccagatgca gtacgaacag
    13441 caccagcacc acgccctctc ccaccagcgt cggcccggcc ccgagcgccg cggcgatcac
    13501 ccccgccagc aagccgccca gggcgtcgaa accgaagcgt gccgacgtga agaaggacac
    13561 cacccggccc cgcagccggt ctggcgcgat gctctgcagc agcatgttga tgcccacgtt
    13621 gcacaccgag atcccgaatc ccaggcccac catcgccgcc agcgccaccc cgaagcgcgt
    13681 tgtcagcccg aagaccagca gcgccgccgc gctgatcgcg gtccctgcgc acaccgcctt
    13741 caccagcccc ggcaccgagc gccgcgtggc cagaaagacc gtcgatccga aggccccaca
    13801 ccccgccgcc ccccacagcc agcccagggt gcgggcgtcg ccgccgaaca cgtcgcgggc
    13861 aaacaccggc agcagcaccg cgtaggacga agccgtcagg ttgagggcga cgaggatcac
    13921 gatcaggctg cgcaccagcc aggtctccca ggcatagcgc accccttccc ggaaaatgtg
    13981 acccatggag ccggccgccc gcggcgtggc gctgtccttc acccgccaca ccgccacgaa
    14041 gagggccacg aaggagcagg cgttgagcgc gaagcacgcc gcctccgagg tccaccccag
    14101 cagcagaccg gcgatgggcg gcccgacgaa gcggccggag ttgaacagca tcgcgttgag
    14161 ggccagcgca ttgggcagat cctcccgccg cgccacaaag gccccgatca gggactggcg
    14221 caggggcgtg tcgaagctgt tgagcacccc caccccggcc gccaggatca ccagcatcgt
    14281 tggccccacc gcctccgccc aggtcagccc ggccaggatc agcgcctggg ccaccagcag
    14341 cacctgcacg cccagcagca ggcggcgctt gtcgtggcgg tcgatccacg cccccgccag
    14401 cgggccgacc agcaactggg gaatcagcgc agcaaaggcc gtgaccccca gcagcgccga
    14461 cgagccagtg agccgataca ccagccaggc catcgccacc tgctggatcc acgaccccag
    14521 gatggacacc gcctggccga agaaatacag gcggaaattg cgatgggcca gcgcccgcag
    14581 gggaccgggc agacgggatg agggttcgac ggatgacatg gaggacaacg cgaggcaccg
    14641 cacgtcgccg gaacggacga cgcacgaaag tgcgcgattg tgcctgagtc gcagtcggcc
    14701 ggcacgacca cggaggacct tgacgtcacg gtccgcgagg atccgccaca tcctcgcggc
    14761 cgtgtcgcct gcgggcagcg acggcttcac gcgggggccg tcgccccacc gcccgaatcg
    14821 cccctgcggt cctttgctcc ggccggacga gatccgcaca tcgccaatgg agaggactgc
    14881 atcccggcac gaccgggacg cagtctgccc tttactgccg accttccagc agacgcttga
    14941 agttgtcgag gatggcctgc cagccgctcc gctgctgctc ggcaggaaac tcgttctccg
    15001 cgtcgaagga gatcttcacg cggaccccgg tggcgtcctc ggagaactcg acccgcgccc
    15061 ggcgatcccc aaaggcgtat tcgagcagac gctcgggcac gacctgggta taggtgccgg
    15121 caaaatcgaa tccgaagctg ccgtccttcg cctccatgcg cgacgagaag gtgccgccca
    15181 cccgaagatc cacgctggcg cgcgtggtgt gccaatcgtc ggaggcggcg ttccacagga
    15241 cgatgtcctc gggcgtcgta taggcccgcc acaccgctgc gaggggcgcc cggatcgtcg
    15301 tctcgaccgt gattttcata gtctctcctg gtgggtcatg ggtgccatgg gatcgaatgc
    15361 gcggaggcgc ccccaccggc atcctgggga gatgtgagct tacgcgcgcc gggcggaaaa
    15421 gcggaagtcc cgcgccgcat ggacaggaac gcctcgacga tacgccccgt tccgtgccgg
    15481 cggcggagac ctcatccgta cggccggccc gcgggccccg gagcatccgg tgggccgtca
    15541 ggacggcgat gtccgcacgc ccgggatcct cccgcacaga tttcccccac gggcatccac
    15601 gatctccagc cggtacccgg cgggccgcac cggcaccacc ggggcctgcg caccgaaaca
    15661 ccacacgccg ggcggctgcg gccccgcacg gcttgcttcg cccagggcat tgatcacctc
    15721 gaaacacccc tgcgggtgct cgcgccctgt cacacggtcc ggcccctctg ccggccggca
    15781 ccggatcgac acctgcaagc gcccgtcggc cgtccacacg ccctccagtt catccgggga
    15841 gactgccacg ggcgccagcg tgccgccctg gccagggaca cccacgccga aggtaagcag
    15901 ccgcagggcc gaacggatcg cgtcgggcgt cgcccggcgg ggcagcgccc cggtccacac
    15961 gaccagcgtc aggtccgccg cagcggcgtc accgtctggc ggcgcgcaca tcctttcgat
    16021 accggccacc ggccaggcct gtgccatctg ctcggaaaac ggccaggcgt ggcgcgccgc
    16081 gccgaattcc agggcatggc cgacctccgc cagctgcggc gcgaccgtcg cccagtcggc
    16141 aagggccgcg tccagactgc gggcgatacc ctccacgggc tggcacaggc tcagatcgtg
    16201 gctcaccacg gccagcgccg gttccccacg cgggcggggc agtcgggtca gtttcatgga
    16261 ggcgcgtcgc tggcgggccg taccggccca tggacggccc gattgacggg cgccgtcctg
    16321 ccgcggagga tgatgcaccg gacgcgcagc ggccggcggc gggcgatgcg tgccggccgt
    16381 tgcggatcca ccctcgagcg aggctcacgc gccctcatgt ccgacaagac cctgctcatc
    16441 gtctatcact ccatgaccga tgccacccgg cagctggccg tggcggtcgc ccatggtgcc
    16501 gcgtgcgagc cggccagcgt cgccgtgcgc ctgctgcggg cgcccgacgc cagcccggac
    16561 gacgtgctgg cggccgacgg ctacgtgttc gccaccccgg aaaacctggc cagcatggcc
    16621 gggatgatga aggacttctt cgaccgctgc tattacccgg cgctcgaccg cgtcaacggc
    16681 cggccttacg ccagcctggt gtgcgccggc agcgacggca gcggcgccgt gcgccagatt
    16741 gcgcgcatcg ccaccggctg gcggctgaag gagatcgcgc cgcccctggt ggtgtgcacc
    16801 catgcccaga cgctggaagc catccttgcg cccaaggtcg tgggggtgga cgcccttgcc
    16861 cgcggcgagg agctgggcgc ggccttcgcc accgggctgg cgatcggcgc cttctaggcg
    16921 gcgcacgccg cccgcagccc gcggccgatg tcgaccgctt cgtccaggcg cggcagcagc
    16981 acggccacga tggacgcgtc cacccgcccc tggccggcca gatcgttcat gaaggcggcc
    17041 acctcgggcg gcggcagccc ggcgcggtag ggtcgatcct gaatcatggc ctggaagatg
    17101 tcggccacgc gcaggatgcg cgcctcgacg ggcagctgct caccctgcag gtggaaggga
    17161 taaccgcggc cgttgggctc ctcgtggtgc caggctgccc agcgggcgat gtcctcgaac
    17221 ccggcgatgt cccgcaggat ctggaaagtc tcgaaactgt gggtgcgcat cacggcccgc
    17281 tcggcctcgt ccagccggcc gggcttgtcg aggatctcgt cgggcacccg cagcttgccc
    17341 aggtcgtgga gcaggccggc gatctcgatc cgttcgcagg ccgcggcgtc catgtccagc
    17401 gccgcggcca gcaaacgcgc cacgccggcc acgccaatcg agtgggcggc cgtgaaggga
    17461 ctcttggcgt cgacgatgca ggcaaagatc ccggccagct gatgcagctc ctgcaccgac
    17521 gccgggacgg ccggcccgtc gaagcggcgg gtgcgaagga aggcctggat ggcccgcggc
    17581 tcctgcatca gccagaagga ttcccgcgcg gaggcccgca gaaaggcgtc caccaggcgc
    17641 ggatcgaagt agtgcccggc gtggccggcg atctcgtcgc gaatcctgcc cgtgtgcagc
    17701 agcagttcgc cccgcgcctg ccaggacgcc gcgagggcat cgacccgatc caccaggtag
    17761 atcaggttcg cctgccgcgc cacgtcgtcg ggtagcccga tggtcgtcag cacctcccag
    17821 cgggtgtggt gatagcgcac cggcagggcg agaggctgca ggggcgggaa tcgcccgagc
    17881 agggcgaagc cggtctcaca gtgatcctgg gcaccgtccc agtcgaagtg gccgaccagc
    17941 ttgccgtggg tgcgcgtgga tgagacaccg atgtcgtgga gcatgcccag atcgaagcag
    18001 aagcggacct ccgcgtcggt caggcccagt tcgcgggcac attcggccgc catcaggccc
    18061 acgcgcttgc cgtgggccac gtcgtccacg cccaccaggt cgagggcttc ggagagggcc
    18121 tggatgacgt ggcggagatc ggtctgggtc acgggcgcgc ctcgctaaag ggagttcgag
    18181 ggagggaagt ccgcggacgc agcgccgggc cggcctccag catgccttat cggacggttg
    18241 gcccgggatc ttgatcgctg cggcatggcc tgcgcggaca acgctgcgct cagcccccct
    18301 gcccgttgct gtcgcgccag aagcgttcca tcgcgatgta ggtgtaggac gccgaccagc
    18361 cgatcaagag cagcccgttg agcgcctcga cgcccgccag cagcctcacc gcgccgccgg
    18421 gcaccacatc cccatagcca agggacgtgt aggtttccgc cgaaaagtac agggagacgt
    18481 cgagggagaa tcggctggat tcgtccagcg tgccgtagcc gccccactgc accagggcat
    18541 acacgaccag cgcatagagc gcgatctcga cgatgtgggc aaagaaggtc gagaagatca
    18601 ccaccagcag gcgcgcccgc gggcggatcg tgagggtcgg cagcagcagg ctcagcccgc
    18661 gcaggacctc ataatggatg accgtggtga cgatcagcag tgccaggcag gccagtacga
    18721 ccatgtgcgc ttccccttgt cccttctttc gctgaggccc ggattatccc atcgggaacg
    18781 gtcagggcgc tgcaccaagg ggaaagcaaa cgtaagggcg atcagctccg ggcgacgcca
    18841 ccagcgaccg ccacccgcgt gccctgcggc gcatgatgcg cggcccggta gaagttcagg
    18901 atgcggcgca cgtattcctg ggtctcggcg taaggcggca cgccgccgtg acgggtcacg
    18961 gcgccctcgc cggcgttgta ggccgcggcg gtcagggcca cgtcgccctt gaactgggcc
    19021 agcagcgtac gcaggtaggc cagcccgccg cggatgttct gttccgggtc gagggcgtcg
    19081 cgcacgccaa agcgttcggc ggtgtccggg atgagctgca tcaggccgac ggcgttcttc
    19141 ggcgaacggg ccgcggcgtc gaagttggac tcggtgcgga cgatggccag ggccaggcgg
    19201 gcatccacgc tgaagcgcgg agcgagcttc tggatcagct gggcgtggcg gcgcttgtcg
    19261 gccggcagcg cgcgcacgta gcgatccaca atgtcctggg gcacgacctc gtcaacgacc
    19321 gccacggccg cctccggcgc cccgccgcgg gtcagacaat cgggcaggcg atcaccggcg
    19381 gggagcccgc ccagcaggct gcgggcgcgc tcgtgcccgc gttcggcggc cacggcgagg
    19441 aggttgacgg ccagctggtt gtcccgggca agcccctgac cgatcaggta caggcggcca
    19501 aggcgatact ggccttccgt gctgccctca cgggcggcgg cgcagtagtg gctggcggcc
    19561 tgcgcgggca ggtgttgacg ttcggcctgg tagccacgct ccagcaggcg tgccacggcg
    19621 ggcgcctcct cgctccattc cggcgcgtcc gccgggacgg cctcgagcgt gccgccgcga
    19681 gccccaccgg tgacggcgag caggagcagg acggacaaca ggcgaaggac gggacgtaac
    19741 atcggggcgc atggagactc ggggcgtccg catcatgccg cccatgccgg cgtcagcgcg
    19801 tgaagcattc gacattcccc cgtacatccc gtccgagcgg tcggtggaac ggtcaatccg
    19861 tcacgcggcg ggcggcgcgg ccccgggcca gcggcgccct cagacgtgct cgatgctcag
    19921 caccacctgg gtctggttga gcagcaggta ggcgatctcg ccaaaggtca tgggcccggc
    19981 gagggaaccg caacggcgtc cttcgaggcc gtggtacagg tagttgagga tgcagttgca
    20041 ggaccacagg ggcggtacct cggcctcggc accggtcggg tcgccgaagc tcaccggggc
    20101 ggccaggcga tagtccacgt cgtcgaagac cggcgcgtag aaatccacgc gccccgcggt
    20161 ctcgtccacg cccttgatgg acacgttcac cgaggccccg ccgtagtcgg ccaccagggg
    20221 caggcgggtg tcggcggcgc gggccttgag ataggccgcg aattgggtct cgacgccatt
    20281 gacctggcag cgctccgccg aaaagccggt ttcgggaaag cgcagggtgt cgccgtcacc
    20341 ttgctgcagg gcattgaaga tgtcgatgcg cgcgtacgcg ctgtcgggca gcggcacgtg
    20401 caggaccacc gcccgctccg tatcgaacgc cagacgtggc cccgccacca cggtgggact
    20461 gaggcgaccg gcgtcgtcca ggtgggtgcc ggcgacccag ccggccaccg gcttgatgaa
    20521 catgtcgggg aaggtgggcg ccttgcgcgc atagagggaa tgcacggccg agaaggccgg
    20581 gatgatgatc aggctgaagc cgttcgccgg ggcgtcctcg cagagatgct ccaggctggt
    20641 gagatcgtag ctgcggatct cggggacgcc cgccatcacc gggatctcgg tgacgaagag
    20701 cagcgcccgg ctggccagcc cgccctcgtc ggccatgaaa taggggatgg tcccgccgat
    20761 ccagtggccg gccggcaggc ggcgcagcag ggtctcgtcg ccggcaatca tcagccatgc
    20821 gccgcggcgg atgagcgtgg ccgcgtcgtc cacactcatc aggcgattgg tcggtgcgtt
    20881 catgcggcct cccggaccag ttgtcccagc tcataggtga gcatgcgctc gttcttcacg
    20941 aagtagcgat gcacgtcccg cagcttcatg cggcgcacgt cggcgtcggc gatcacggcc
    21001 acctgggcac gggtccggga cagcagcagc tgcaggctca gcacccgcac atccagctgc
    21061 cgtcgccctt ccagcaggtc cagccagacc gggtcgtccg ccgacggcac gcggctgccg
    21121 gcgacacccc cggccaccgg cgcctccgtc cgcggcacgt tccgcaaggg ctcgacgccg
    21181 gccatcgccg gagcgggcgc ctcctggccg cccgacccga gctgaccgtt ctccagcgca
    21241 taacggtagc gccacagctc cgccatgggc agatcgagaa aggcgcacac cgccgccatg
    21301 ctcacgcctt catggagcag cgcgagcacc tgccggtcca gcgcccgggt gaagggcatc
    21361 ccggcctcgc cggcccaggc ctggcggggc atttcgccgc ccaggggcgg gcgcacgtgc
    21421 acacggatct tccagccacc gaagccggta tgccgccaga ccacctccgg accggattcg
    21481 acgggcttgc cccgaccgaa gccgaaccag ctgcgcggcg cctcgacgcc gatccacaca
    21541 tccatgcagc gattgaattc atccgcccgg gcctcccgga cggtccacgg ttcctggacc
    21601 tgcagcaact gccgccacag tgcggacgca tcggccatag ccacctcgct cgaaggaaga
    21661 cgcccggagg cgccagaacg gagaaccggc cgcaagggac gtgccagaat ccgtccgttt
    21721 gccggcagag cgcccccgca ggccgtcacc gcccgcgctc cgcgcgacac ctcgcccatg
    21781 ccaggcagat tgcaccgtcg ggctgcgcga tgccgcgtcg caccatgtcc cgccggtgaa
    21841 caccctgcgt ccccaacctg ctgcaaatcc gatcaacatg cccgcggcgg ccccgccctg
    21901 cgcgacggga cggccgaagc gtggcaggat gccgcgagtc ccgaagccct ccgccatgac
    21961 ccgcaccgaa cgtctcttca ccctgatgca gatcctgcgc cgccaccgtc agcccgtgag
    22021 cggtaccgac ctcgcccgcg agacgggggt cagcctgcgc accctgtacc gggacatcgc
    22081 cacactgcag gcccagggcg cccacatcga tggcgaggcc ggcgtgggct acgtcctgcg
    22141 tccgggtttc ctgctgcccc ccctgatgtt cagcgcggag gagatcgacg ccattgtgct
    22201 cggcgcacgc tgggtgaccg aacgtgcgga cccggccctt gccgccgcgg cgcgggacgc
    22261 cctgagcaag gttgcggccg tcctgccggc cccgctgcgc caccagctcg acgacgtgcc
    22321 cctgctgatc ggcccggtca ccgacagtgg tcgtgatgcg cacacggtgg cgcagctgcg
    22381 cacggccctg cgcaacgagc gcaagctgtc gatcgactac cgcgacgagc acggcgccgc
    22441 gagccagcgc gtcatctggc cctgcgccct gggcttcttc gaacgtgtcc acgtcctggt
    22501 cgcctggtgt gagcggcgcg ccgatttccg ccatttccgc gttgaccgca tcgaacggct
    22561 ggacctgctc gacgccggtc tgccgcgtcg ccgtcaggcg ctgctgcggg aatggcgcgc
    22621 gcgggaaggc atcaccggct gacgccgcgc cgcgccgtgc gcgctgctgc cggtttctga
    22681 cagcccgccg ccgcagaatg ggtccacccg ccggcctgcc gccggacggc ccttcgaccc
    22741 cgaaccgagg aaccctgcca tgccgcaccc gaactacatc ctgctctacg tgaccgaccc
    22801 gagcgccagc gccgccttct acacggcgct gctggcgtgc ccgccggtgg aggcctcgcc
    22861 caccttcgcc ctgttcgccc tcgattccgg cctgatgctg ggcctgtggg cgcgcggcag
    22921 cgtcgagccc gcggcctccc tgggcggcgg aggcggcgag ctcgccctca ccgtcgccag
    22981 cgaggccgcg gtggacgcct gctttgccga ctggcgggcg cgcgggctca cgatcctcca
    23041 gcccccgacg cacatggatt tcggctacac ctgcgtcgtg gccgatccgg accaccatcg
    23101 tctgcgggtg ttcgcaccag cccgtccgta acgggaacct gcggaccgga gaaccggtca
    23161 tccctggcaa agcccgggcg tgccgcctcc atgacgtggc cggcccgcag cgggctgcca
    23221 tcaatccccg gagccgcccc atgatccccg tcgcacgccc ccgtcatccc gcgtctcgcc
    23281 tttttctaag cgcccggctc ctggccgccc tggccgccct gtccgtcgtg gccccgccgt
    23341 ccgcagccgg ccccctgcgt gaacggctgc gggagcgcct gcaggcagac gacgcgtctg
    23401 gcggaccgct gagcgatctg ggcggtggcg aagccctgag ctgcagcgac tggtcccgcc
    23461 gggtggcgcg ggtgcaggct ctggccggcg ccggcctgcg cggaccggcc gcggatctgt
    23521 ccgacgtggc ctacggtgcc ggcccccgcg agcagctcga cgtctttgtc ccgcatgacc
    23581 gctcacatgc gggcggcgcg cccctcatcg tcatggtcca tggcggaggc tggtgcgtcg
    23641 gtgacaaggc catgaccggc gtgacgaccc acaaggtcga ccgctggaca ccacacggct
    23701 tcgtcttcgt atcgctgaat taccccatgg tgggcgacgg tgccgatgcc cgcgcccagg
    23761 ccgcagccgt cgcccgtgca gtggccttcg tgcagcgtca tgcggccgac tggggcgccg
    23821 atcccgcgcg ggtgattctg atgggccact cggccggggc ccatctggtg tcgctggtca
    23881 acgcggatgc cgagctgcgg cgtcaggcgg gcgtcgggcc cctcctcggc accgtctcgc
    23941 tggatgcggg ggccatcgac gtggtgcggc agatgccggt cgtttatccc ttcctgaaga
    24001 cccgctaccg ggaggccttc ggcacgtccg aagccgggtg gctggccgcc tcgccctatc
    24061 accgcctcgg gcgcgacgcc gccccatggc tgggggtctg ctccacgcag cgtcaggacg
    24121 acccctgcgg acaggcccgc gcctatgccg acaagagcgt ccgtctgggc ctccgtgccg
    24181 aagtgctccc cgaagccatg aaccatggcg ccctcaatgc ggacctgggc ctgcccgggc
    24241 cgtacaccca ggcggtggaa cgtttcctgg ccggcctcga cccggcggtg gcggcccgcc
    24301 tcgggcgctg agtcctcact cgcaggcagc ccggcgcggg cagacacctg cgggtcgggc
    24361 cgcggatcag cggttggccg cgtgcctgcg gttagcctcc ctccgccggc gcgccggcag
    24421 cggtagccgt cgaaagacgc atcgccccgc cgggcaaggt cggccgcccg gaacggccgt
    24481 gtacatgaaa aaaggccggg acacgtcccg gcctccttgt tcgcgtgagc gacgcggcag
    24541 cacttactgc acgcggacct ccacgcgacg ggcttcggcc tcgtcggcac cgccgagggt
    24601 gatggcgggc ttgcgcagct tcagcttgtc ggcggccacg ccggcggtcg ccagggcgtc
    24661 cttgacggcc agggcacggg ccttggagac ttcggcgttc accgcggcac cgccggtttc
    24721 gtcgtggtag ccggagatca gcaccagctt ggcgggctct tcgtgcagct tggcgacgac
    24781 cttggccagc tcttcggcgg cgttggccgg cagctcggcc ttgccggagt cgaagtagag
    24841 cttgaccagc ggctcgccca cttcggcgat ttcgggctct tcggccacca cggcgggctt
    24901 ggcagtctgg gcctgaccgg tcacataggt ggtcagaccg atcaccagac cgatgaccag
    24961 ggcgatcaca ccgaaaagaa tgcccagaac gagattctgg ttgtcgtcgt cttgcgtcat
    25021 tgcacgatcc ttcgggaagt cagtgggaat gaattcagcc cgcgagtata gcggtttacc
    25081 ctgacgatct cgcgatttcc ccctgcaatt cacaaaaaag gcgacgtcac ccgctgcggc
    25141 ccgccggcca ccggatgcat ggacgcccgc acgcggcggg cgtccgccac cccccggcca
    25201 tcgccgggcg gtgggacatc aggccctggc gaaggcgatg cggccgttgt cgcaggacac
    25261 ccggatggtg tccttggcag cgaactggcc ttccaggatg cgccgggcga gcgggttctc
    25321 gatgtgctcc tggatcgccc gcttgagggg ccgcgcgccg aacaccgggt cgaagccggc
    25381 cttggcgagc tcggccaggg cctcgtcgct gacctccaga tgcatctcga gccgcgccag
    25441 acgcttttcc agatagccga gctggatgcg cgcgatggac gcgatgtgct tctcgtcgag
    25501 ggcgtggaag accaccacct cgtcgatgcg gttgatgaac tccggccgga agtaggtctt
    25561 cacctcggcc atcaccgcca gcttgatcac gccgtaatcg tcaccggaca tctgctggat
    25621 catctggctg cccaggttgc tggtcatcac gatcacggtg ttcttgaagt ccaccgtgcg
    25681 gccctgaccg tcggtcatgc gcccgtcgtc gagcacctgc aggagcacgt tgaagacgtc
    25741 cgggtgggcc ttctccacct cgtcgaagag aatcacggaa tacggcttgc gacgcacctg
    25801 ctcggtgaga tagccgccct cctcgtagcc cacgtagccc ggcggcgcgc cgatcaggcg
    25861 ggccaccgag tgcttctcca tgaactcgct catgtcgatg cggatcaggt gctcctcgct
    25921 gtcgaagagg aattcggcca gggtcttgca cagctcggtc ttgcccacac ccgtggggcc
    25981 caggaacagg aaggagccgt agggccggtt ctcgtccgac agcccggcgc gggagcggcg
    26041 gatggcgtcg gccaccaggc gcacggcctc gtcctggccc accacgcgct gatgcaggcg
    26101 atcttccatc ctgagcagct tgtcgcgctc accctgcatc atcttggaca cggggatgcc
    26161 ggtggcgcgg ctcaccacct cggcgatctc ctcggcgccc acctgggtgc gcagcagcct
    26221 gtgggccggc ttggcgtcgc cggaggcttc cgccttcttg agctgggctt cgagctgggg
    26281 cagcgtgccg tattgcagct cggccacctt ctcgagctta ccttcccgtt gcagcctgac
    26341 gatgtcggcc ttcaggcgat cgatctcttc cttgatgtgg gccgaaccct ggacggcaga
    26401 cttctcggcc ttccagattt cctccagatc ggaatactcc ttcttgatgc gggcgatctc
    26461 ctcctcgatc agcgcgaggc gcttctggga ggcttcgtcc ttttccttct tgaccgccag
    26521 ctgctcgatc tccagctgga tcttgcgccg ctcgagcttg tccatcacct ccggcttgga
    26581 gtcgatctcc atcttgatgc gcgccgccgc ctcgtcgatc aggtcgatgg ccttgtcggg
    26641 caggaagcgg tcggtgatgt agcggtggga gagctccgcc gcggcgacga tggccgggtc
    26701 ggtgatgtcc acgccgtggt ggagttcgta cttctcctgc agaccgcgca ggatggcgat
    26761 ggtcgactcg accgagggct cctccaccag caccttctgg aagcggcgct ccagggcggc
    26821 gtccttctcg atgtacttgc ggtactcgtc cagcgtggtg gcgccgatgc agtgcagctc
    26881 gccgcgggcc agggccggct tgagcatgtt gcccgcgtcg atggcgccct cggccttgcc
    26941 ggcgccgacc atcgtgtgga tctcgtcgat gaacaaaatg atgcggccct cgtccttggc
    27001 gatgtcggtg agcaccgcct tcagacgctc ctcgaactcg ccccggtact tggcgccggc
    27061 gagcagcccg gccatgtcca gcaccagcac gcgcttgccc ttgagcgtct cgggcacctc
    27121 gtcattgacg atgcgctggg ccaggccctc gacgatggca gtcttgccca cgccgggctc
    27181 gccgatcagc accggattgt tcttggtgcg ccgttgcagg atctggatcg cgcggcggat
    27241 ctcgtcgtcc cggccgatga ccggatcgag cttgccctgg cgggcccgct cggtgaggtc
    27301 cacgcagaac ttcttgagag cctcgcgctg gctctcggcc tcggcgctgc ccacgctctg
    27361 gccgccgcgc accgcctcga tggcctgctc cagggccttg cgggcgaggc catgctcctt
    27421 gagcaggcga ccggtctcgc ccttgtcgtc ggcgagggcc agcaggaaca tctcgctggc
    27481 gatgaactcg tcaccgcgct tctgggcttc gcggtccgtc acgttgagca gattgcccag
    27541 gtcacgcccg acggacacct cgccgccatg gccttccacc ttgggcaggc gggtcaacgc
    27601 cttggccagc gcggccctga gggcctgacc gttgacgccg gcgcgctgca gcagcgacgc
    27661 ggtgctgccg tcctcctggg acagcagagc catcagcagg tgctggggct cgatgaactg
    27721 ttgatcgttc cccacagcca ggctctgcgc ctcctgcagg gcttgctgga acttggtggt
    27781 gagtttgtcg aaacgcatgg aatgcctcct gaacgggtct gaggcctaga tgggggaggc
    27841 gccggcagtt tcaatggggg gcgcccacgg gcaagaccgt tcacgcgccg ccgtggcaaa
    27901 ccgcgccgcc ggaacgcctg ccgtccgacc actgctccag attcccgtgg cagggggtgt
    27961 tacatcgtcc ggcccccgcc tgcgccccca cccgatgcct caattcgggg cattgccggg
    28021 ccgcgctgcg ggtaccgcgc ccgccgcccc ggagagcctt gcaaaccgtt ctttaaaaag
    28081 gtgttgctgc ggtgcaacat aaatcgaaaa aatcgacgtt tttttgatct gcgcccattg
    28141 tcggtgcatg agcctgtgct atcgtttttg tgcaagcccg ggggagggaa atcgatctgg
    28201 gtttcggact cacatcaacc aaacgaagtg gagatcaaca tggctaccaa gcaagaacag
    28261 cagttcaacg aaatccagaa gaagaacctc gaatccgcca tgcgtctggc gcagctgtcc
    28321 atcgaaaact cccagcgcat catggaactg caggtcggca ccgccaagac cctgtttgaa
    28381 gaaggcgttg ccaatgccaa ggccctgtcc accgccaagg atccggccga agtcatgacc
    28441 ctgcgcaccg cctacgccca gagcaccacc gaaaagatgc tgtcctgtgc ccgcgagatc
    28501 gccgagatca ccgccaagac ccaggctgaa gtcggcaaga tggtcaccga gcagctcacc
    28561 ggtggcggcc aggacatctt cgaatccctg caaaagatgt tcaagggcat gcccatcggc
    28621 gacaccaacg ccctcggctc catccagagc gccatggaca ccgcccgtgc tgcttacgag
    28681 cagatgacca aggcctccac cgaagccttc caggccttca ccaagatgtc ccccaagaag
    28741 taatccacgc ttccttgcgg catcaagcga cggcgcctgc gggcgccgtt tgctttggtc
    28801 ggacgggcgc gacgcggcgg gcaggcggag ccgcctgaaa cgcgtctgtc acgtggggcg
    28861 ggcatgctgc acggcacgat ctccccggcc cgcggccatg acggaaaaag cccgcatcat
    28921 cgacgccctc ggcgaaccga ccctgctcct tcccgccctg ctgggtgaag cgctcggcgc
    28981 cagcgaccgc atcaagtact acctcagcct gctccaggcg gcccgcacca gcgccctcca
    29041 cccgcagcag cccgcggcgc cgctgatggc cgaacgctgc gccgcaggca tcgacgacga
    29101 gcgtctcgac accctgccgg cccgcgcgga acgcgaccac gacggacaga tccacatccc
    29161 cgacgccggc atcctgtgcg accacatcgt ccgcgatctg gacgccctgc tcgcgccgat
    29221 ccgcagtgcc ggggcggagg ccgccgtggc cctgtcggcc cgcgccgcgg ccttgcgcag
    29281 ccagccctgg tgcgaatcct gcgacacctt cgacgccggg cagttctcgc gcctcacctc
    29341 tgccgaccgg gacgccggcg acagcgtgca cctgctggtc ctggacgccc acaaggagct
    29401 caaccggctc cagacccgca tcgccagcga agacatcgac ggcgccgccg cctacgacat
    29461 tcgccccgcc gaccgcgccc tgatcgccgc cttcatgcgc ggcgtcaatg ccagccgccc
    29521 cctcaagctc gaccaccccg gcctgggcac caccgccacc cgcagcggcg gccgcctggt
    29581 cctgcagaac gacatcggca ccaccgacgc ccacgtgctc gtggtgcacg tggagaaccg
    29641 ccgcgtcacc ctcacctaca ccgacgtcca tctccagcgc ctgctgttct tccagagcct
    29701 cttcgcccac tgggcggtga gctgggagga cacccgttcc cgtcgcgacc ggggcatgga
    29761 ggacggtctc taccacgtct cggtgggcac cttcagcgcc aacaacgacg ccggcctggg
    29821 tgactacctg agcttcctgg gttcgcgtct ggtcttcctg atcgactgga accgtgcccg
    29881 caaacgcctg cgtctgctcc tgcccaagaa ggaaaccctc gatctgctgc actgggcggc
    29941 cgacgagaac gtcggccaca tggcctggct gcgcagcggc ggggaacagc tggtcttcga
    30001 cgccgtggcc ttcgccgcac gcaccccacc caccttcggc gcgcgcctgg acgacatgct
    30061 cgaccccggc aaggcggtcg gcttcatgca gagcgtgctg cgcgcctgca cgcgcggatt
    30121 cctcgaacgg cgcccggacg aggtcgtacg cgacgagatc cgcgccgagc tcgccacctg
    30181 cttccgcagc gcccgccagc agatcatcga catcgtggcc gagcacgccg ccttctccat
    30241 cgagatcgcc accggcatcc gcgactgcct gcccgacctc accggccccg acgccgccgc
    30301 ccgcatcgcc gccaacgcgg cccgcgcccg ccagtgggaa cagcgcgccg acgaacgcct
    30361 caaccaggcc cgggaactgc gccagcagca gggcgagtgg gacgatttct accatgacct
    30421 ggtcgcccgc gccgacgaca tcacggacga actggaggaa gccgccttcc acctcaccct
    30481 gttcccgccg gaggcaccgt ccgccgaact cctcgagtgc ttcgccaccc tggccgggct
    30541 ggcggtgagc ggcgcccagg agttcctcaa ggccgtcgag acggcccgcg gcctgcgccg
    30601 cggcggtccg cgcgaggaca tccaggactt cctcgcggcg atccaccaga ttctggtggc
    30661 cgaacggcag agcgacgaca cccaccggcg cttcaagcaa tgcctcgtcg ccacccccgg
    30721 cgaattccgc agccactatc cgtgggcaga gttcgcccgc aacctggaaa ccgcctgcga
    30781 cgccctgctc catgccgccc tgcaactgcg cgaccacatc atgctcacgg tcacccgcga
    30841 ctgaacgcat ggacctcaac gcccacctct acgccatcgg cctgccaggg ccccgccagg
    30901 aaggctccgc cgacgccatg ggcttcaagg cctggaacct ggcgcggctg gccgccatcg
    30961 gcctgcccgt gccgcccgcc ttcgtcctcg gcacgcccct gtgcgcccgc caccgggcag
    31021 accctgcggc cttccggccc cacctgcgca ccctcctcga aacccagatc gagcgcctgg
    31081 aggctgtctg cgggctggag ttcggatccg agcgcaaacc cctgctggtg tccgtgcgct
    31141 ccggcgccgc cgtgtccatg cccggcatga tggacacggt gctcgacgtg ggcctcaccg
    31201 acgccaccct gcgcggcctc ctgcgcgtca ccggcaaccc ccgcctggcc tgggattcgt
    31261 accggcgcct gatccagcag ttcgccgagg tggtgcacgg cgcccccgcc gagcccttcc
    31321 agaccctgct gcacgatgcc atcgcccagg ccggggtgag ccgcgcccag gatctggact
    31381 tccgcgatct gtcgcgcctg tgccggcact accaggacgc ttacgcgcag accgtcggcc
    31441 aacccttccc gcaggatccg gcgcagcagc tcctcgacgc cacagcggcc gtgttcgact
    31501 cgtggatgag cccgcgcgcc gtggcctatc gccggctgca gggcctcgac gacggcctcg
    31561 gcaccgccat caccgtccag cgcatggtct tcggcaacgc aggcggcacc tcgggcgcgg
    31621 gcgtcggctt cacccgcgac ccggccagcg gcgagaaccg cctgtacctg gacttccgct
    31681 tcaacgccca gggcgaggac gtggtctccg ggcgccacgc cgccgccgag aacggccgca
    31741 tggccctcgt cctgccctcc gtgctcagcc gcctgcagtc cgttcgggaa atcctggagc
    31801 gggagttcgg cgacgtgcag gagtttgaat tcaccgtgca ggacggcgtg ctctacctgc
    31861 tccagacacg cgccgccaag cggaccgact gggccgccct gcgcatcgcc gtggaccagg
    31921 tcaccgccgg cgcatggtcg cccgccaccg ccctgcggcg cctcgacggc gtggatctgg
    31981 ccggcatcgt ccagacccgc atcgaggccg cgggtaccga ccgggaactg gcgaccggca
    32041 cgcccgccag catcggcgtg gcgagcgggg tcatcgccct cgacgcggca gcagcggaac
    32101 gcttcgccca gaccgggacc gaatgcatcc tggtgcgcga cgagacccgc agcgacgacc
    32161 tcgccggcat cgccgccgcc cagggcgtga tcacggcgaa gggcagccgc accgcccacg
    32221 ccgcggtggt cgcccgtcag ctcggcaagg cctgcctggt gggctgcaat ggcctgcgcg
    32281 tggacctgga gcgccgccgc gtggtcttcg gcacccagtc cctgcacgag ggcgagcgca
    32341 tcaccgtgga ctgcgggagc gggcgcgtgc tggccggtgc cgtgcgtgcc ttcgtcgaac
    32401 gcccggacgt ctggctcgac caggtcgccc gctggcgcgc catcgccggc cccgccctcg
    32461 tgggctgaag gcccgggtcc acgatcggcg gtgccgatgc gccggggcga gccaggctgg
    32521 cggccgccgt ccctcgaaca gccggccacg ccagcgggtc cgcctcagct gatcgtcacc
    32581 gccgcgctgc ctgcattcac cgtcgcggtc accgtccggg caatcaccgt gctcgacggc
    32641 gcggccgtgc ccaccagccg gttcacctgc ttgaagacac agctcaggcg acccggcgtc
    32701 accgtgacca cggcatagcc ctgggcgtcg gtcgccgccc acttgatcca cgggttggcg
    32761 cagatgcccg ccagttgctg ggccagcccc agcaggtcgg tgttgaagct gctgtccgcc
    32821 ttgagcgcgg tgaggaccgc ggtcgtggtg gcgtccaggg acgcttccgg cacacccttg
    32881 gcggccaggg cgccgcgcag gggcacacgg gcctgttcgg ccagttcgtc cagcgtcggc
    32941 acgtgaccgg ccatggtgaa gtcaaatacg ttgaagcgca ggctcacggt gccgacgccg
    33001 ctcaccggaa tgctcagggg atagtagatc agggtggcca gatccgcatt cacggcgccg
    33061 acggaatcgg cgtagaaggt gaacagggag tcggagctga tcccggcggt caccagatcg
    33121 accatcactg gcgtgccacc gctggcgtcg aagtcgtcgt acaccacgcc ggcgtcgaaa
    33181 caatgcaggt cgccggtgag cgccaccaca ttctggattc cgttgccctt gagatgggcc
    33241 atcagcgcct tgcgctccgc gttgtagccg tcccactgat cgcagtcgaa caggaagcgg
    33301 gtgaagaagc cctgcagtgc ggcagccttg ccggaactct gcacgaagct cgatgcgacg
    33361 cccttggcct ggacgtccgg cttgatgtag ccgaaggcga tggtgcgggc ggcggcggcg
    33421 acttgcgcgc tggtgccgtt ggccgcagcc gcggcagtga aggcggccac cgcgatggcc
    33481 gcctggtgag cggtgaggcc ggcccccgtc gctgcggtgc caggattgcc cgccgtcgcc
    33541 gctgcggtgg cgatggccag cgctgcggcg gccgccgtcg cctggcttgc ccctgcagtc
    33601 atcgccgcca ccagcgcgcc ggccacggcc gcgttgccgc ccaccgccgg cagcgcaccc
    33661 tggatggaat tcgccagcgt actcaccgcc tgcagggcaa acagggtggc gatggcattg
    33721 gtgccgtcca gttgcatgcg cagcagggac acctcattgc accacagctt ccaggtggcg
    33781 ggcgaggcgg tcatggtgga cttccaccag tcccgctggg tcttgcccag gatggaaacc
    33841 ggcgccagcg ggtcggccga accggcggcc gccatcttgg ctgcctcgaa gctgtcgcgg
    33901 gtgctctggg gcactacgta gcgggagccg atggagccca gcgcggcgcc ggtggcgggg
    33961 ttgggcgtgg cctccggcac cacgtggtcg gcacggtaca ggcgctcgtc ggtcaccacc
    34021 agatgggcca gcgtacccca ctggaggtcg cgatacaggc ggatcgtatc gaagctgagc
    34081 gtgctctcgt ccaagcgcac gtcagccggc atgaactcga accaggcccg gttggcgctg
    34141 cgccggcggg cggtctgatg ggggttgtcg ccgctcaggg tggcgctgtt gaagctgccg
    34201 ttgtcgtagg tctcggcgtc gccccagcaa tcatcggaga actcgtggtc gtcccaggtg
    34261 gcgacgaagg caaagcgctc atgcacggcc tgcaggcgcg gatcggtgcg gtacttcttg
    34321 tacagatagc ggtaatcggc cagggtcagg gcgtagcgcg ggccgctggt gcccgattcg
    34381 taagcgccgt cgggcagcac caggaggtcg tggcgggatt ccaccgcgcc gctctggaag
    34441 ctcgcaccca ccgtctcgta gatgtagtcg cccagatgga cgatcaggtc cacgtcctca
    34501 ttcctgacga tgtagtcgaa cgccccccag tgattgacgc tccagtcctg gcaggtcatg
    34561 aaggccaggc gcagctgatc gacgctggcg cccttggccg gcgcggtgcg gaagcggccg
    34621 acccgggaca gcaccatgcc ggcctcgaac tggtagtagt aggtggtggc gggcttgagg
    34681 ccggtgagct tgttgcgcac ggtaccgtcg taggccgcat agaccgggat ccagttgtcc
    34741 accgcgaggg cgccggccag caccgtggtg gtgccgaggc gtgccgaatt gtcggcttcg
    34801 gtcaccttca cgcggatcga aaaatcgccc gtatcggacg ccgtggtcac gtcatcggcc
    34861 gaggacgcca ccacgcgggt ccacagcacc gcgccctcgg gcgacggatc gccggaggcc
    34921 acgctctggg ggaatttcca gccgctgccc agggctgccg gcacggccag gccgccggag
    34981 tcgccaccgc agcctgccag gccgcccagg gcgacggaga ccgtcagaaa tccgctgttg
    35041 cgaaggaagt tgcgtcgatc catggcctgg ggctcctgtc agaaagttca gcccgacagc
    35101 ctaagccccg agaatttcag gcgtgttgcc ccctccatga agggaaattc atgcgcccat
    35161 cacgcgctgt tcacgaagag tcgccagcct cggagctttt cctgaccgga gactttcatg
    35221 cgcgccccct cccctgcatc cgctgccctc gcgaccttcc gccgccaccc cctgcacttc
    35281 gcctgcctcg ccaccctggg cctgtctgcc tgtggcggca gcgattccct cacgcccctg
    35341 cccaccctgg acggcaacgc gccgctggtg gtcgcccacc ggggcgcctc cggctacctg
    35401 cccgaggaga ccctggaggc ctacaccaag gccatcgacc tgggcgccga cgtgatcgag
    35461 ccggacctga tttccaccaa ggacggcgtc ctcatcgccc gccacgatcc caacctggcc
    35521 tacagcaccg acgtggccag ccacccggaa ttcgccgacc gcaagaagac cacccaggtg
    35581 gacggcgaga cccagaccgg ctggttcgcc agtgacttca ccctggccga gatcaagacc
    35641 ctgggcggca tctccaccga tgccgaacgc ccgcagcagt acaacggcct gtacaagatc
    35701 gtcaccttcc aggagatcat tgatctggcc aaggccaagg ccaaggaaac cggccgcagc
    35761 atcgccgtct accccgagac caagaacccc acctaccacc gcgacctggg cctgcccctc
    35821 gaagacaccc tcatcggcct gatcaacctg gccggctgga acagcaagga cgcgccgatc
    35881 tacgtgcagt ccttcgagcc ctccagcctc aagtacctgc gcgccaaggg cctgaagacc
    35941 aaactgatcc agctcatcga cgccgacgac gtggacttca agaccggcgc cctcacctac
    36001 gccgccccct acgacaagcc ctacgactgg ttcaaggccg gcgacacccg cctgttcagc
    36061 gccatggtca cgccggcggg cctcgccgag atcaagacct acgccgacgg gattggcccg
    36121 tggaagcgtt acatcgtcac cgtcaagggc acgctcgacg ccagcggcaa cgtgaaggac
    36181 atcaacggcg acggcaagat caacgacgcc gacaccacca gccagcccgc caccacgctg
    36241 attgccgacg cccacaaggc cggcctgttc gtgcacccct acaccttccg caacgagcag
    36301 cgccgcctcg cctccgacta caagggcgac ccgaagaacg agtacctggc cttctaccgc
    36361 ctcggcgtgg atggcctgtt ctcggatttc accgacacag ccatcgccgc ccgcacggcc
    36421 tacctgaagg agatgggccg ctgaggcagc gcgaccgagc cgccgcggcc ccgcggggcg
    36481 gcggcggtga acgcacaaac gcccggacac gtccgggcgt ttgtgttcga cggagggcgc
    36541 cgacgatcag ccgaagcggc cggtgatgta gtcttccgta gccttctgct tgggcgacag
    36601 gaagatctgg tcagtctcgc cgtactcgat cagctcgccc aggtacatgt aggcggtgta
    36661 gtcggagatc cgcgccgcct gctgcatgtt gtgggtcacg atgacgatgg tgaactcgtt
    36721 cttcagctcc gccaccagct cttcgatgcg ggccgtggag atcgggtcca gcgccgaggt
    36781 gggctcgtcg aggagcacca cttccggctt caccgcgatg ccgcgggcga tgcacagacg
    36841 ctgctgctga ccgccggaca ggctcatgcc gctctggttg agcttgtcct tcacctcgcc
    36901 ccacagggcc gccttggtga gggcccactc cacccgttcc tccatctcgg agcgcgtcag
    36961 cttttcgtgc agggacacgc cgaaggcgat gttgtcgaag atggacatgg ggaacggggt
    37021 cggcttctgg aacaccatgc cgacgcgcgc ccgcagggtg gtgacgtcca ccttcgggtc
    37081 gagcaggttc tgcccgttga gcaggatctc gccctcggcc cgctggttgg ggtagaggtc
    37141 gtacatccgg ttgatggtgc gcagcagcgt ggacttgcca cagccggagg gaccgatgaa
    37201 ggccgtgacc cggtgcttct ccatctcgaa gttgatgttc ttcagcgcca ggaattcgcc
    37261 gtagtagaag ttgaagttcc ggaaggcgat ctgcgccggg cccttgatga caccctggct
    37321 ggattgagtg gtggcttgtt gcatggcaga ggctcgctta gtggagtttt tcggcgcgga
    37381 acatcacgcg ggcaataatg ttgaggccca ggaccccgag ggtgatcagc agcacgccgg
    37441 cccaggccag acgttgccag tcctcgaagg ggctcatggc gaacttgaag atcgtcaccg
    37501 gcaggttggc gatcggctcg ttcagatcca cgctccagaa ctggttggac agggcggtga
    37561 agagcagcgg cgcggtctcg ccggcgatgc gcgcaatggc cagcagcacg ccggttacca
    37621 cgccgctgcg ggcggcgcgc agggtcacgc gggagatcac gatgtgcttg ggcgcgccca
    37681 gggcgaaggc cgcttcccgc agggaaccgg gcaccagcat cagcatgttc tcggtggtgc
    37741 ggatcacgat gggcagcacc agcagggcca gggcgacggc gccggaccag gccgagaact
    37801 tgccggtggt ggcgacgatc accgcataca cgaagagacc gatgacgatg gacggcgccg
    37861 acaggagcag gtcgttcacg aagcgggtga cgcggcccag ccaggtgtag cggccgtatt
    37921 ccgccaggta gatgccggcc atgatgccga tcggggcgcc gacggcggtc cccagggcca
    37981 ccaggatcag gctgccgaca atggcgttga gcagaccgcc ggtctcggcg ccgggcggcg
    38041 gcgtcatctc ggtgaacagg gacagggaca ggccaccgag gcccagctgg agcacggtga
    38101 cgaggatcca ggcgagccag aacaggccga agaccatggc ccccatggag aggccgaggg
    38161 cgatctggtt ggtgagtttg cgacgggcga gcaggtccat ggatcagcgt ccttcgttgg
    38221 cggacaggcg gagcagcagg agcttggaca gcaccagcac gatcagggtg atgacgaaca
    38281 ggatcaggcc cagctccatc agcgccgagg tatgcaggcc ccgctccgct tccgcgaatt
    38341 cattggccag cgccgaggtg atgctgttgc ccggcgcgaa cagcgaggcg gtcgacagga
    38401 agttggtgtt gccgatcacg aaggtcaccg ccatggtttc cccgagggca cggccgaggc
    38461 ccagcatcac gccgcccacc acaccgttct tggtgtaggg caggaccacg cggaacatca
    38521 cctcccaggt ggtacagccg agaccgtagg cggactcctt gagcatcgcc ggcgtcacct
    38581 cgaacacgtc gcgcatcacc gatgcgatgt agggaatgat catgatcgcc aggatgatgc
    38641 ccgcacacag caggccgatg cccagcgacg gaccggagaa cagcgcgccg agcagcggaa
    38701 tcttgcccag ggtggcgccc agggcgggct cgatgtattc gccgaagatg ggcgcgaaga
    38761 ccagcaggcc ccacatgccg aagacgatgg acggcacggc ggcgagcagc tcgatggcgg
    38821 tgcccagcgg gcggcgcagc cacaggggcg acagttcggt gaggaacagg gcgatgccga
    38881 agctcaccgg cacggccacc accagggcga tgatcgacga cacaagggtg ccgtagatgg
    38941 gaatcagggc gccgaactgc tcggcgggcg ggttccattc cgcggtcacc aggaaggccg
    39001 ggccgaaagc gacgatgctc ggccacgacg caacggtcag ggccacaatg atgccggcca
    39061 gcagggccag ggccagcacc gcgaaggtgg tcgagatgcc gccgaagacc ttcgcgccca
    39121 cctggctgac cggccgtcgc gcaggctggg cgacgtccgt ctgcgacact ttcatgtcaa
    39181 tggcctcagt attcatgaac gtgatcccaa ggataagcga atggggccgg agagtcgcct
    39241 cgtccggccc cctgactggc tgtgcgaacc ttccggtccg tcttacttgc cggagaagac
    39301 cgccttgccg gaggcatcct tgatgccggc ccaggaggca cggatcagct tgacggtgga
    39361 ttcgggcagg ggcacgtagt ccagatcggt ggccagcttg gcaccgttgt ggtaggccca
    39421 ctcgaagaac ttgagcgctt caccgccctg aaccggcttg tcctggacct tgtgcatcag
    39481 gatgaaggtg gcgccggtga tgggccagct gtccttgccc ggcttgtcgg tcaggatctc
    39541 gtagaaggcg ttcttgctcc agtcggcgcc ggcggcggcg gccttgaagt tctcgtcgtc
    39601 cggcagcacg aaggcgccgt ccttgttctt cagggacgca aaggtcagct tgttctgctt
    39661 ggcgtaggcg tactccacgt agccgatggc gcccggcaga cgctgcacga aggcggacac
    39721 gccttcattg cccttgccgc ccaggcccag cggccactgc acggtggcgc cttccttgac
    39781 ctggttcttc cagtcggcgc tgaccttgga caggtagttg gtgaagatga aggtggtgcc
    39841 agagccgtcg gcgcgacgca ccacgccgat gtcgctggcg ggcaggttca ggttggtgtt
    39901 gagggcgacg atggccgggt cgttccactt ggtgatcttg cccaggtaga tgttggccag
    39961 gatctcgccg gtcagcttga gctggccggg ctgcacgccc ggaatgttca ccacgggcac
    40021 cacgccaccg atcacggtgg gaaactgctg catgccgtcc ttctcgagct gctcggggga
    40081 caggggcatg tcggaggcgc cgaaatcgac ggtcttggcg gtgatctgct tgatgccgcc
    40141 ggaagagccg atggactggt agttcacctt gttgccggtt gccttctggt aggcgtccgc
    40201 ccacttggcg tagaccgggg cggggaagga agcacccgcg ccggtgatgt cggccgcgaa
    40261 ggcggtcgag gcgaggacgg cacccgcgga agcgagcagg gaacgggtca cggcgttcag
    40321 catgaagaat ctcctgagtc tggggggtgg gatccggggc agcggggcgg gacgttctct
    40381 gtggcaccgc acaaaacact tgccctgatc tcgcgaccga agcttagatt ttgaatgtga
    40441 cgggattgtg acaattcaca atccggcaac ggcgtgaccg gaacacgtcc ctcgatcccg
    40501 cccgatcggt tgcggcggcg caacacccta tcactttctg catagcttcc tgccagagaa
    40561 ttcaacgccc cctccgtcac agcggcgcaa cagagcgatg ggattattta catccggttc
    40621 catatttcac cccccgcttt ccggagactc ttcatgcgac acatcagcct tctggccgcg
    40681 gccgtttctg cggccctact ggccggctgc ggcggcaaca gcagcaccca ggagacgccc
    40741 gccaccaacg tcatcttctt cctcggtgac ggcatgggca tcaacacgct caccgcggcg
    40801 cgcatctaca aggtgggtga agacggcgac ctgaccatcg acaccctgcc cgagacggcc
    40861 ttcgtccgca cctactccaa caacgcccag gtcaccgact cggccccctc catggccgcc
    40921 tacatgaccg gcgtgaagat gaacaatgaa gtcatctcca tgtccgccga caccatggct
    40981 tttgacgcca gcggcaacaa ctacctgagc ggcaccgaca gcacctgtcc ggccagcggc
    41041 aacggcagcg ccgtcaccac cctgctggaa ctggccaaga aggccggctg gagcaccggc
    41101 gcggtgacca ccacccgcgt cacccacgcc acccccgcgg tcacctactc ccacatctgc
    41161 caccgcgacg ccgagaacac catcgccgcc cagatggttc cgggcggcag cggttacaac
    41221 agcgccctgg gcgacggcct caacgtcctg atgggcggcg gccgccagtt ctacctgccc
    41281 accgcctctg gcggcaagcg taccgacggc cgtgacctga ccgccgagct ggtcaagcag
    41341 ggttacacct acgtcaagaa caagaccgaa ttcgatgccg ccgccgcggc ctccaccgac
    41401 aagctggtgg gcctgttcac ctccagccac atgagctacg acatcgaccg cgaccccacc
    41461 gtcgagccca gcctggccga gatgaccacc aaggccatcg acatcctgtc caagaacaag
    41521 aagggcttct tcctgatggt ggaaggcggc cgcatcgacc acgccctgca cgagaccacc
    41581 gccgccaagg ccctgcagga caccgtggcc tttgacgacg ccatccagac cgccctcacc
    41641 aagatgaagg ccgtcgatcc caccctggcc aacaccctga tcgtcgtcac cgccgaccac
    41701 gaccacaccc tggtgctcaa cggctacgcc aagcgcaccg gcgccaccac cgccagcaac
    41761 cccggcatcc tcggtctgct gaagaacttc gtcagcggcg ccaacgccac cgacagcggc
    41821 ggcctgccct tcaccatcct gggcttcggc aacggcgaaa accgcccggc cacccgcagc
    41881 gccctcaccg acgcccaggt ctccgtcaag acctaccacc aggaagccgc cgtccccatg
    41941 gccgccggca gcgaaaccca cggcggcacc gacgtcttcc tcggcgccat cggccgcggc
    42001 gccgacaagt tcaccggcgt catcaccaac accgaagtct tcggcctgat ccgcagcgcg
    42061 gtcggtctgt gagccgcgac aggagccctt ccatgaaatt ccagatcaag acctgcgtcg
    42121 ccgccctcgc cctggtcggc accacctccg cctttgccgc cggcgaagcc accaacgtca
    42181 tcttcttcct cggcgacggc atgggcccca ccaccgtcac cgccagccgc atctacgagt
    42241 atggcgaggc cggcaagctg aacatggagt ccctgacccg caccgcgcgg atcaagacct
    42301 actccaacga cgcccagacc accgacagcg ccccgtccat ggccgcctac atgaccggcg
    42361 tcaagatgaa caacgaagtg ctgtccatga gcgccgacac caaggcctac gccagcaccg
    42421 ccaccaacag cggctacgtc agcggcgccg acagcacctg cccggccacc ggcaacggca
    42481 cggccgtcac caccctgctg gagctgtcca aggccgccgg caagtccgtc ggcgcggtga
    42541 ccaccacccg catcacccac gccaccccgg cggccaccta ttcccacatc tgccaccggg
    42601 acggcgagaa cgacatcgct gcccaggccg tgcccggctc cggcaagttc aacagcgccc
    42661 tcggcagcgg cgtggatgtg ctcctcggcg gcggctggcg cttcttcaag cccaataccg
    42721 tcaccggtgg cagccgtacc gatggccagg acctgaccgc caagctgcag accgcgggct
    42781 acacctacgt ggagaccggc accgccttca aggccgtcga tgccaccgcc accagcaagc
    42841 tggtcggcct cttcaaccgc gaccacctca actacgagct ggatcgggtc aacaagagca
    42901 tcgacgaacc cagcctcgcc gacatgaccg aaaaggccat caagatcctg cagaagaacg
    42961 gcaagggatt cttcctgatg gtggaaggcg gccgcatcga ccacgccctg cacggcacca
    43021 acgccaagcg cgcactggaa gacaccatcg ccttcgacga agccatcaag cgcgccctgt
    43081 ccctggtgga tctgaagaac accctggtcg tcgtcaccgc cgaccacgat cacaccatga
    43141 ccttcaacgg ctactcccac aagggcaacc ccatcctggg caaggtcacc gacatcaccc
    43201 aaaccaccgt caagaagacc ccggtcctcg ccgccgcggc cgacggcaag ccctacacca
    43261 ccctggtgtt cggcaacggc ggcggccccc gcaaggccac ccgggacgac ctgaccagcg
    43321 tggacaccac caccgacgac tacctccagg aagtcgcggt aaacctgggc tcggccggca
    43381 ccgaaaccca cggcggcggc gacgtcatgc tgttctccgg cggcgccggc agcagcggct
    43441 tcaagggcac gatggtgaac accaaggtgt tcggcctggt gaagtccgcc ctcggcctgt
    43501 aaagcatcga ttgccatccg ggatcggcct gctgcgtaga agaagttggt cccatttccc
    43561 gcccgacccc accggtcggg cggctttttt tgagacattc cccgtcacga tgaagaagct
    43621 cctcctcgcc gccctcctcg gcgccctcac cgccggcgcc cgtgccgacg acctggccct
    43681 gcgcatcgaa cacgacagca ccgtcgtcgg tgccgacggc gtcacccgca acgtgcgctt
    43741 cgccgaacgc ctgatccgcc gcgacgcgca gagctggctg gcgcgcatcc tgccccccgg
    43801 cgcccacgag gacgccgaac acgccgccgg cgccaagggc cacaagcaca tggacatcgc
    43861 cgccgccacc cgctgggtgg tccgcgcccc cgacggcaag ctgcgggtgc gcctggtgaa
    43921 cgaccacgac cggatgatcg tggacatcgc acccgtcgat tacgccaaca tcggcttcga
    43981 cggccgctgg agcaccgcgg cccagctcat cgacccggag cagatccgcc agatgaagcc
    44041 tcttgcccgc agcgccccgg ccggcacccg ctggtacgag agcggcacgc ccgacgcccg
    44101 cgtgcaggta ctgtgggacg aaaaggacaa gtacccgcga cgcatcgaat ccagcaacgc
    44161 tgccggcacc cgccgcagcg tcctcaacgc cacccgcgag gccctgcccg ccgtgctgcc
    44221 gtggacgcgc ctggacggct acgccctgaa ggaatactcc gacctgctcg actaggaccg
    44281 gcgccgccgc ccgaggcgcc ccctgtcacg aatctgtcac acccctggaa ccccggcgcc
    44341 gcaccatggc gtggaatcca tccgtctgca aggtgtcccc atggtcttct tcgaactctt
    44401 ctccaacgcc cccgacatcg tccgcgtccc tgccgggaag gccctcttcc acgaaggtga
    44461 agaaggctgc tgcatgttcg tgctgaccat cggcagcgcc gaggtctatg tgaagaaccg
    44521 ccgggtcgag ctccttgtgc cgggcaacat ggtcggcgag ctggccctgg tcacccgcgg
    44581 cccccgcacg gccaccgtcc tcgccctgga agacagcgag ttcgtgtcca tcgacgagga
    44641 ccgcttcaac tatctggtcc agcacacgcc cttcttcgcc ctcaacgtca tgcgcctgct
    44701 ggccgagcgc ctggctgcgg cgtccagtgc cctgccggcg gtggaggacg tctgacccgc
    44761 gggcctcagc gcggaccggc gttcccgtcc ggtgcaaact ccccgcacca ggaaaagctt
    44821 gtcaccgccg gaaagcgcca ggtgtggtgc tccgccgggt gctcgccggc aaaggatggt
    44881 gggtagcggt ggcaggtgcc gctggtggcc ccattgctct gaaagtacgc gcactcggcg
    44941 caggtgggat ggttcagtcg gggatcggac atggtcgggt cgggaaaatg ttgatcgcgg
    45001 ccgtcgccgc ggcgataacg ctgccgctcc attatgaggg cccgatgtga cagaaattcg
    45061 gcactgcagc atcgccacat caccgtaacg cgcccgtcac ccggccgcaa catggcgccg
    45121 ctatcgtcgc ggcatttccg cacggccggc cccgaccggc gccgccccga accgtcgcca
    45181 tgccccgtcc gcgtctccgc gccctgcgca ccgctgcatt cctcgccctc ctgtccctcg
    45241 gcctcagcgc ttgctccccg cgccagctgg tggtgcgcag cgtcgccgac cagttggccg
    45301 gccaggacgc cggcagtgag aacgatccgg agctcgcccg ggaagccagc gccttttacc
    45361 tcaagctgtc cgaatccgtg ctgcgtcagg atccggacca cctgcccctc gccaccgcgg
    45421 tggccggcgg cttcacccag tacgcctacg ccttcgtcgc cttcgacgcc gaccgcctcg
    45481 aagcccgcga cagccgcggc gcccaggccc tgcgcgagcg ggccgcgcgc ctctaccggc
    45541 gcgcccgcga ccacgccctc gccaccctcg aacgccatcg ccccggcctc gccgccgaac
    45601 tggccacagc gggccccgag cgtcctctgg ccaaggatct cgttcccctc gcctactggg
    45661 gcgcggcagc ctggggcgga ctcgtcagcc tctccaagga cgacccggac accgtcgccg
    45721 acctgccccg tgcgatccgc ctcgcccgcc tggcctggct cgccgatccc gcctggggcg
    45781 acggcacgct ggccagcctg atgggcagtt tcgaggccgc ccgccccggc ggcagcgccc
    45841 gccaggccga agcctatttc gacgacgccc tgcgccttgc cgccggtcgc agcgccggtg
    45901 ccttcgtcgc caaggccgaa ggcgtcgccc agcccgccgg cgacaaggcc gccttcctcg
    45961 acctgctgcg ccaggccgag gccgtgcgcg acacccccgg cagccccctc accctcgcca
    46021 acgaagtcat gcgccgccgc gcccgctggc tcgccgagaa cgccgacgac ctgttctgaa
    46081 cgtcggcccc tcgcccctca cccggaaaac gccccatgcc gctcaccgcc cgccgccacg
    46141 ccctgcgcct gctcgccacc ctgcccgccc tggccctgcc cggcctcgcc gtggccgccg
    46201 gcccacagat gcgcatcggc accctcgccc ccaagaactc cctctaccac cgccagttgc
    46261 tggagatcgg cgaagcctgg cgcgcggccc agggcgaagg cgccaagttc accgtctatc
    46321 ccgacggcag ccagggtggc gagaccgaca tggcccggcg catgcgcatc ggccagctcc
    46381 agggcgccct gctgtcggtg gtcggcctgc gggagatcga gccctccatc tctgccctgc
    46441 agaacatgcc cctgatgttc cgcagctggg acgaggtgga ttacgtccgc gaaaagatcc
    46501 gccccgccat ggaaaagaag ttcctcgaca agggcttcgt cgtcctcacc tggggcgacg
    46561 ccggctgggt gcgcttcttc tccaaggaag ccgccgtgcg cccggacgac tacaagaaga
    46621 tgaagttctt cgcctgggcc ggcgaatccg agcagcagga catcatgaag agcctcggct
    46681 acaccccggt gcccctcgaa accagcgaca tcctgccgtc catccagacc ggcatgatca
    46741 acgccgtccc gtccacgccc tacttcgccc tggccagcca ggtctatgcc accgcgaacc
    46801 acatgctgga catcaactgg gcccccatcg tcggcgccct ggtggtgact aaaaaggcct
    46861 gggacgacat gacccccgcc gcccagaccg ccctgcgcga cgccgctgcc aaggccggcg
    46921 cccagatgcg ccagcaggcc cgcaaggagg tggacgaagc cgtggaggcc atgaagaagc
    46981 gcggcctggt ggtccacacc cccacccccg agcagatgaa ggaatggaac gacctggcgg
    47041 acaagctcta tccccgcatc cgcggcaaga tggtgcccgc cgacaccttc gacgaggtcg
    47101 tgtccctgct caagacctac cgcgccggca aacgctgaag gacgtcatgg cgcgccggcc
    47161 gtgcccacct tcgtgctttc cgcctcccac ttccgcgccc tcccccccaa tgcgccgctt
    47221 cctgcccgac gaggccctcg cctccgccgc cctcttcctg atgaccctca tcccgctggt
    47281 ggagatggcc gtgcggccgc tcttcggcaa gggcatcgac aacgccccgc tgctggtcca
    47341 gcacctgggg ctgatcctcg ccatgtttgg cgccctgctg gcggagcggg gcggccacct
    47401 cacctccctc ggcaacagtc tgggcagcgc ccgcgatccg cgcgtccgcg ctgccgccgg
    47461 cctttttgcc catgctgccg cggccctgct gtgcggcctg ctcggcgcgg caagctgggc
    47521 cttcgtcgcc agcgagctgg acgccggccg cgagctggcc tacggcctgc cggtgtggac
    47581 ggtccaggct gcaatgcccc tcggcttcct gctcctgggc atgcgcctgg gcgcccgctg
    47641 cgcgccggcc ggcctcgcgc gccccctggt gggcgcctcg ctggcaggcc ttggcttcct
    47701 gctggcccgc cacttcgacg gcagcaccct gagcttcgcc cccttcgcgg tcgccctgct
    47761 ggccgccctg ctggccggcg cccccatctt cgcggtgctg ggcggcctcg ccctgggcct
    47821 gttctggtcc gaaggccagc ccctcgcctc ggtgcccctc agccactacc agatcaccgt
    47881 caacccgtcc ctgccggccc tgcccctgtt caccctggcc ggcctggtgt tcgcccgcag
    47941 cggtgcggcc ggacgcctcg gcaccctgtt caccgcctgc ttcggcggcg gcgccaccgg
    48001 cagcgcggtg gccgcggcgg tgctgtgctc ctgcttcacc gccttcaccg gcggcagcgg
    48061 cgtcaccatc ctcgccctcg gcggcctgct cctgcccctg ctgcgcgacg cgggcctgcc
    48121 tgaacggcgc ggcatcagcc tggtcaccag cgccagcgcc ctgggcgtgc tgctcgcccc
    48181 gtcggtgccg ctcatcatgg tcgccatcat cgcccgggtg cccatcaaca ccatgttcct
    48241 ggccggcctg ctgccggccc tggtgatggt cctgtgcctc ttgctggtgg gcggctacct
    48301 gcgctccacg ccccgcgccg gggcgcccgt cgccgccccg tccgcagggg tcccgtctgg
    48361 cgtgggcctg cgcgcggcca tctgggcggc ccgctgggag atccttgccc cggtggtggc
    48421 catcggcgcc ctcgccagcg gcatcgccac ccccaccgag agcgctgccc tcaccgccgc
    48481 ctacgccctg ttcacccagg ccgtgctgca ccgggaggtg gacgccaccc tgctgcgccg
    48541 ctgcctggcc gactgcgccc aggtcatcgg cggcgtgatg ctgatcctcg gcatggccct
    48601 gggcctgacc aactacctgg tggatgcggg catcccggat gccgccgtgg actgggtgca
    48661 ggccaccctg cccaacaagt tcgccttcct ggtggccctc aacctgttcc tcttcgtggc
    48721 cggcgccctg atggagatct acgccgcgat cgtcgtgctg gtgcccctgc tgctgccggt
    48781 ggcggtcagt tatgacatcg acccggtgca cttcggcgtg atcttcctgg ccgtcatgga
    48841 gatgggcttc ctgtgcccgc cggcagggat gaacatcttc ttcgcctccg ccatgttcgc
    48901 ccgccccatc cgcgaggtag ccgtgtcggt gctgccggcc ctgctggcaa tcttcctcgg
    48961 cgccctgatc atcgcctggg tgccctggct ggccaccggg ctgccggcgc tgttcgccct
    49021 gcactgaccc acacgcggcg gcacccgctc aacgcggaca gaatcgatat cccacgccgg
    49081 attcagtgag gaggtggcgc ggccgcgacg ggtcgtcctc gatcttcttg cgcagctggg
    49141 ccatatggac gcgcacgtag tggacgtcct ccgcgtgccc cggcccccac accgtcagcg
    49201 ccagatggcg gtgcgtcagc accgtgtccg ggtgggaggc gaggcaggcg agcaggcgga
    49261 actcgatggg cgtcaggtgc accggcaccc cgtcccgctc gatcagccgg cgcccgaagt
    49321 ccgcccggag gcgcccgaag ctcatcacgc cgggcgtgcc gtcgcggggc gccgcacggc
    49381 gcaactgggc ccgcacccga gccagcagct cggccgcacc gaacggcttg acgaggtagt
    49441 cgtcggcccc cgcatccagc gccgccacct tctccgactc ctgggtgcgc gccgacagga
    49501 cgatgacggg gatcgccgac caggtccgca gttcgcggat gaaggccttg ccgtcgccgt
    49561 cgggcaggcc cagatcgagc accaccagat ccgcctggcg cgtgcccgcc tcgatcagcc
    49621 cgcgcttcac ggagtccgcc tcgaaggcct gcaggtcggc gctctcgagt gccatgcaga
    49681 cgaagcgccg gatcttcggt tcgtcctcga tcaccaccac gaccggccgg ggatcagtgc
    49741 ccatggggcg cgtcctccgc aagcccgccc atgcccggca ccgccggcgg ctgcccggcg
    49801 ggcaggctga acaggaagcg ggcgcccccg tccgcccggt tctcggcccg gatggtgccg
    49861 ccgtgggccg cgacgatggc gcgacagatc gccagcccca gccccacgcc gggggtcgcg
    49921 ctctccgact ggccgcgttc gaacttcttg aagatggcct cctcccgccc cgccggcagc
    49981 cccggaccgt cgtcctcgac ccagatcgtc acaaagccgc ccgcgggccc ctccgcaccg
    50041 atgcggaccg gggtgcccgg cggcgtgtac ttgaccgcat tctccaccag attcgacagc
    50101 acccgctcga tcaacaccgt atcgaactcc agcaggggca gatcgtcggg cagacggacc
    50161 tccaccggat gacggaagag ggccgactcc agccccttca gcgcgctgcc gaccacctcc
    50221 tcgagcggct gccactgctt gttgagggtc accgtgcccg cctgcagccg cgccatgtcg
    50281 agcagattgc tgaccagggc gttcatgcgc tgcacctcgt cgcgcatcga ctcggccagc
    50341 tgcaccaagg ccggcgcagg tgccggccgg gtgagcagca tcatgtccac cagccccacc
    50401 agcgccgaca ggggcgtgcg cagatcgtgg gagatggccg acagcaggga attgcgcagg
    50461 cgctcggact ccatctgcag tgtcgtggcc tgggctacct ggatgtaatg gacgcgctcc
    50521 agggaaatcg cgatgaggcg cgcgaaggtc tccagcaggc gatgctgctc cggcgcgccc
    50581 atgcgccccg gatggcgggg ctccagcacc agcacgccgc ggatgcgcat gggcgccttg
    50641 aggggaacgt agagcagcgg actcgccggc agcgtatccg tgccgcagcc ggcctccgcc
    50701 tcgtggtcga aggcccactg gcagatgccc gcgtccacgc cgctgagccc gcctggtacg
    50761 gcgagcggcg ccagcagacg gttggcgtcg tccgccagca ccagcgtgca gcgcgccgca
    50821 aaggtcgtcc gtgagaagcg cagcgcgatc tctgcgatct gctcgggcat cagggccccg
    50881 gagagatcgc gagacatctc gtagagcgcc cggatgcggt attcgcggcg gctcgccacc
    50941 cgcgcctgca gcttcaggcc ggcggtaagg ttggccgtga tgaggccgga cgccagcatc
    51001 accgcaaagg tgagcacgta ctgcacgtcg ctcaccgcaa acgagaagcg cggcggcacg
    51061 aagaagtaat cgaaggccgc cacgttcagg aaggacgcca ccaccgccgg ccccaggccg
    51121 tagcgcacgg ccgtcaggac caccgcgagc aggaagacca tggcgatgtt ggccaggtcg
    51181 aaatgcccca gcagcagcgt ggcgaccacg gtcgccaggg cgcccagggc ggcggtcatc
    51241 gcgaaggccg tccacggcgc ccgcagccgg tccagccacg actcgctggt gggcgcctcc
    51301 accgcggcgt cccggcgggc ctcgtcgcgg gcaaccagga tcacgtccag gtccggcgcc
    51361 tgccggccga tctgctcagc catggagcgg tgccatgggc gccagccgcg gggatgatcg
    51421 cgccccacca cgatgcgcgc gagattgtgg tcacgggcgt aggccgcgat gcgctccgcc
    51481 gcatcggccc ccgccaggct cgccgtctcg gcgccggatt cctgggccag gtgcaggttc
    51541 ttgaggatgc gctggcggtt ccgctcgctc aggtggcgca ggtccggcgt ctccacgtag
    51601 atcgcatgcc agggcacctc gaggcgcccc gccaggcggg cgcaggcccg caccacgcgg
    51661 tcgccgcctt cgccggggcc gacgcaggcc agcagggatt cctgcgtcgc ccacaccgtg
    51721 ctcaccgccg aatcgcggcg gtagcgcacc atctcgctat cgacccggtc cgcggtgcgg
    51781 cgcagggcca gctcccgcag agcgatcagg ttgcccttgc ggaagaagtt ctggatcgcc
    51841 cgctcggcct gctggggcag atagaccttg ccctccttga ggcgctggag cagttcgtcc
    51901 ggcggcagat ccaccagcac cacttcgtcg gcggcatcga aaacatggtc gggcaccgtc
    51961 tcccagacgc gcacgccggt gatgccgccc accacgtcgt tgagcgtctc cagatgctgg
    52021 acgttcaccg tggtgtagac atcgatcccc gcatcgagca gttcctccac gtcctgccag
    52081 cgcttcgggt ggcgcgagcc ctccacattg gaatgggcca gctcgtccac caggatcagg
    52141 gccggctgcc gcgccagcgc gccgtcgatg tcgaactcgg cgagggtccg ccccttgtag
    52201 gccacgctcc gcggcggcag cacgtccagg ccgtcgagca gtgccgcggt ctcgcgtcgg
    52261 ccgtggctct ccaccacgcc caccaccacg tcgacgccct gggcccgctg ctggtgagcg
    52321 gcgcacagca tcgcgtaggt cttgccgacg cccgccgacg cgccgaagaa gatcttcagg
    52381 cggccgcggg cggcgcgccc ctccgcctca cgcagacggt ctaggatccg ctgcggatcc
    52441 cggcgcgggt catccattgg tgcgcgtcgc gatgatgccc ggccgcagcc cggccatgcc
    52501 gaccgccctc aacgggcgca cggaaactcg ctggacaggg cctcggagac ctgctgggcc
    52561 gcgctgcggg acgtacgggg cggcggatcc ttgggcagat gacgggccac ggcctggaac
    52621 agatgctcct ccgtcaggtc cgcgggcagg caccactgcg tccccgaacc ggcgtcggcc
    52681 acacccagca catagccctt cacgaacaag gccgtgccga ccggcacccg gctgcgcagc
    52741 acgctctcga gttcctgcag gtcagcgtcg ctgcggaagt aaccggcggc ctggactgcg
    52801 cccccccagc ccagcacccc caaacagacc agcgctccga cccaccggcc caggtccccg
    52861 cctcgcgctg ctgtggcggc gcgtccggcc atgccgaacg cctctctgat cccattcatc
    52921 gtgtcacctc ccctcgacgg gaaaacgttc atccagtgcc cggttcaggg ccagcacatg
    52981 gacccgcggt tcaccgaaca gcccccactg gcgcccctcg gtgtgggccg cgaccagcga
    53041 ctccagcgcc gccggggcga tgccgcggag ccgcgcgatg cgcccgatct ggtaggccgc
    53101 ggcggccggg ctgatgtgcg gatcgaggcc gctgcccgac gcctggacca gatctgccgg
    53161 caccggggcc ggattgcccg ggtcggccgc gcgcagaccc tgcacgcgcg cctggacggc
    53221 ctcggccagc gccggattga gcggccccag attcgaaccg gacgacgcgg ccccattgta
    53281 cggctggggc gccgtggccg agggtcgccc ccagaaatgc cgtggatcct cgaaggcctg
    53341 accgatcagg cgggagccgg ccacggcgcc gtctgccagg atcagactgc cggccgcctg
    53401 cgcgggaaag acccaccccg ccaccgcggt gacggcagcc ggatacacca gccccgtgag
    53461 aaggctcaac agcccgcaca gcatcagggc gggacgcacg atggtgttca tgtccggatc
    53521 tcctcaggcc aggccgctgg cggccagagc catgtcgatg agcttgatgc cgacaaaggg
    53581 cgccagcagg cccccgagcc cgtagatcgt cagattgcgg cgcagcagca gccccgcgcc
    53641 ctggggccgg tagcgcacgc cgcgcagggc cagcggaatc agcacgatga tgatcagcgc
    53701 gttgaagatc accgccgaca ggatggccga agccgggctg gcgagggcca tgacgtcgag
    53761 cgcctccagc tgcgggtagg tgctcacgaa ggccgccggc acgacggcga agtacttggc
    53821 cacgtcgttg gcgatgctga aggtggtcag cgcgccgcgg gtcatgagca tctgcttgcc
    53881 cgtctccacc acctcgatca gcttggtcgg attggaatcc aggtccacca tgttgcccgc
    53941 ctccttggcg gcctgggtac ccgtgttcat ggccaccgcg acatccgcct gggccagagc
    54001 cggcgcatcg ttggtgccgt cgcccgtcat cgccaccagg cgcccttccg cctgatgctg
    54061 gcggatcagg gccagcttgg cctccggcgt cgcctcggcg agaaagtcat ccaccccggc
    54121 ctccgccgcg atggcggccg ccgtgagccg gttgtcgccg gtgatcatca ccgtcttgat
    54181 gcccatccgc cgcagttcgc cgaaacgctc gcggatgccg cccttgacga tgtccttgag
    54241 ctccaccacg cccagcacgc gggcatcctc cgccaccacc agcggcgtgc tgccgcgccg
    54301 ggcggtctct tcgacgatgg ccgtcacgga cgccggaaag tggccgccga gtgcctccac
    54361 gtggcgccgc agcgcctcgc cggcgccctt gcggatgcgt gtgccgccca ggtccacgcc
    54421 gctcatgcgg gtctgggcgg agaaatgcac gaactgcgcc cccagggcct gcaggtcatg
    54481 ctcccgcaga ttgaagcgct gcttggcgag caccacgatg ctgcgcccct cgggcgtctc
    54541 gtcggccagg gaggccagct gggccgcccg ggccagcgcc tgctccgtga cgccttccgc
    54601 cgggatgaac gccgccgcct gacggttgcc cagggtgatc gtgccggtct tgtcgaggag
    54661 cagcacgtcc acatcgcccg ccgcctccac cgcccggccg gaggtagcga tcacgttggc
    54721 ctgcatcatg cggctcatgc ccgccacccc gatggccgag agcagcccgg cgatggtggt
    54781 ggggatgagg cacaccagca gcgccaccag caccgtcagg gtcacggggg cgccggcgcc
    54841 cgccacctgc acgctgaaca ccgaaaaggg cagcagggtc accgtcacca ccagaaagac
    54901 cagggtcagg gccaccagca gaatggtcag ggcgatctcg ttgggggtct tctggcgccg
    54961 ggcgccttcc accatcgcga tcatgcggtc cacgaaggtc tcccccggat tcaccgtcac
    55021 ccgcagcacc agccagtccg acagcaggcg cgtgcccccc gtcagcgccg agaaatcccc
    55081 gccggcctcg cggatcaccg gcgcggactc cccggtgatg gccgactcgt ccaccgaggc
    55141 gaccccctcg atcacctccc catccgccgg caccgtctcg ccggcctcca ccagacacac
    55201 gtcgccacgc cgcagcgcgg tggccgacac ctgctgccac ggcgccccgt agcggggcgc
    55261 cgtcagccgc ttggccggcg tgtcctgctt cagcccccgt aaggcagccg cctgggcctt
    55321 gctgcgcccc tccgccaggg cctccgcgaa attggcgaag agcaccgtga accacagcca
    55381 cagggccacc gtgaagatga atccggccgg ggcctcgccg tcccccgcca gggcctggat
    55441 gcccagcacc gtggtcagca ggctgccgcc atagacgacg aacatcaccg gattgcgcca
    55501 ctgcacggcc ggattgagct tgcggaaggc atccaccagc gccggccgga gcagatccgg
    55561 gtcgaacatc gagagcgtct tgcgcgtcat ggtcggcctc ctttcagtgc gccgcgtaca
    55621 gggcaaggtg ttcgacgacc ggcccgaggg ccagcgccgg cacatagttg agggccccga
    55681 cgagcaccac cgtaccgatc agcagcacca cgaagagcgg cccatgggtc ggcatggtcc
    55741 cggcgccggg ggccagccgc ttcttcgcgg ccagatgccc cgccagggcc agcacgggca
    55801 cgatcacacc gaaacgtccc agccccatca ccacacccag cacgctgttg tagaacggcg
    55861 tgttcgccga caggccggcg aaggcgctgc cattgttgtt ggccgccgaa gtgaaggcgt
    55921 acaggatctc cgagaagcca tgggcgcccg gattgcccac gcccgcgccc cccgccggca
    55981 ccagcaccgc caccgcggtg ccggcgagca ccagcaccgg ggtgacgagg atggcgatgg
    56041 acaccatctt catgtcgtgc acctcgatct tcttgcccag gtactcgggt gtgcgcccga
    56101 tcatcaggcc ggcgatgaac accgccagca ccgcaaacac cagcatgccg tagagcccgc
    56161 tgcccacgcc gccgaacacc acctcgccca gcatcatgtt ccacagggtc accaggccac
    56221 ccagcggcgt gaacgagtcg tgcatgccgt tcaccgcgcc gcacgaggcc agggtcgtga
    56281 tcaccgcgaa cagggccgag tcgtcgctgc cgaagcgcgt ctccttgccc tccaggttgc
    56341 ccgccgcgcc ctcgatcccg agggacgcca gcagcggatg accctgggcc tcggcgtgcg
    56401 cgccggccac cgccatggcg acgaagagca gggtcatggt ggccaggatc gcgcgtccct
    56461 ggcgcgcgtc cccgaccatc cgtccgaagc tcacgcacag ggccgccggg atcaggaaga
    56521 tcagcagcat ctccacgaaa ttgctcaggg gcgtcgggtt ttcaaagggg tgggccgagt
    56581 tggcgttgta gtagccgccg ccgttggtcc ccagcatctt gatcgcctcc tgggacgcca
    56641 ccggccccag ggacagcacc tgggtctgcg tccgggccgg ctccgtgagg acacggccgg
    56701 acgcatcgat caggggcttg cccgtcgcat cgaggcgtgg cgcgtcatag tgcgtgagct
    56761 ccagggtggt cgccagccga tcgccgccca ggtcctggac cacgccctgc cccaccagga
    56821 acaccgccac cacgcccgac aggggaagca gcacgtacaa caccatccgg gtcaggtcca
    56881 cccaggcatt gccgacggtc gcggccgaat gccgcgcaaa gccccggatc agggccagcg
    56941 ccacggccat tccggtggcg gccgacagaa agttctgcac gctcaggccc agcatctggg
    57001 tcagcggact catcgtcgac tcgcctgcat acccctgcca gttggtattg gtggcaaagc
    57061 tgatcgccgt gttgaaggcc gaatccggac tgaccgccgc caggccctgg ggattcagcg
    57121 gcagcccgtc ctgcaggcgc tgcagcgcat acaccgccag cgccccgacc aggttgaacg
    57181 ccaggatggc gagcgcgtag gtccgccagc ccatctcctg ctcggcgcgg acgccgcaca
    57241 ggcggtagat cagcgcctcg atcgggcgcc ccggacgcag cgcccgcccc ccctgccctt
    57301 ccgccacggc ggcgatgtag cgccccagcg gcacggccgc ggccaggagc accagcaggt
    57361 acagccccag caggacccac gcgtggctgc tcatgagaaa tcctccggac gcagcagggc
    57421 caccaccaga tagaccagca gcccggcggc cagcaccgcc gacaccgtca gcatcgcgct
    57481 catgaccgcc gctccaggcg atcgcagacc accaccaggc cgaacatcag ggcccacagc
    57541 accgcgagcc caccgccgta catgacatcc atctcaccgc tccctgccca ctgaacacgc
    57601 ccccatggta gggcgccgtg tttcaagaag ctgcaaagat gcggggccgc ggtatcaaga
    57661 aatcatcaag atcgccctcc cggaaggctg cgacacgccc ccgaacctat tccaccaccg
    57721 cccccagccg cgcccagcgc accccctccg gcccggacga ccaccacacc tggcgcgccc
    57781 ggcccgccac gtcggccatg ggcacggggc cgaagcggcg cgaatcggtg gtggcgtccc
    57841 ggttatcacc cagcacgaag acctgccccg gcggcaccgt cagcaccaca tccggcggcg
    57901 cctcctccgg ggcgcgggtg gagccggtcc ctccgcgcag cacctgccag cggcgcccgt
    57961 cgagggtctc cgtcaccgca ccggacatca ccgggtcggt gctcagggcc acgccattca
    58021 cccacaccgc cgcgccccgc gtctccaccc gatcgcccgg caggccgacg atgcgcttga
    58081 tgtacagcct ggtgcggtca ttggggtagg tgaacacggc cacgtcgccg cggcggatct
    58141 gctgcttgca gccggggcag ttgtagcgct tgtcggcgaa caggatgtcg ccccgcagca
    58201 cgctgggctc catgctcgcg gaggggatcg agaaggattc catctggcgg ctgcgcaccg
    58261 tgtccaccag caggggcagg gccagaccgt tgcacaccag gaaggccacc gcgtaggccg
    58321 cgccggtctg ccaggggcgc ggcaggtagt ccggccgcgc ccgcgcgccg cgccaggcat
    58381 ccaccacggc gtagccccag gtgcccagcg ccgcgaccac gctcaccccc agggccggca
    58441 tcgtccaggc cggcggcagg tagagcccca ccagggccat gcccggcacg cagagaaagg
    58501 caaacgcgag aaaaagccac acggccttgt ccagctcgcc gttgtagaac tgcccgtagc
    58561 ccggcaggac cagggacatc agcgcggcgc gcaggggctg acggggcgac acatcgaaca
    58621 tgatcggttc tcctgatgat tcagccggca gtcgtcaccg accacacggc aaaggcgaga
    58681 ctcaacagct gggcgacgcc gaacagcccg gcgcccacgg ccgtcagcca ggccgccgcc
    58741 cgcgcaaacg gccgcccacg gccagccggc gcacccgtcg gtgcgcggga cggcgggatc
    58801 ggctgcggga cggccggcag cgccgccatc acccgcgccg caaaatccgc gggcggcgcc
    58861 gggaagtcct cccgcagcag cgcctccacg gcgggatcga gcacggcatc ggccggccgg
    58921 tcgaagggat cagtacgcat cggaaggctc cagcaaacgg cccagacgct ggcgcgcccg
    58981 ggtcagacgg gtcttggcgg cagcgacgga acagccctcg atgcgcgcga tgtcggccat
    59041 ggccagctcc tccacatagg ccagggcgag gaggctgcgg tccgccaggg agagttgccg
    59101 cagggcggcg tgcagacgct ggcgctgctg cgccagtgcc agcacctcgg gcggctgggg
    59161 gcgcggacag gcgggctccg gcgcatcctc cgccgcgggg cgctcgcgga catggtgact
    59221 gcgccccagt tcgttgatcg ccaggtggcg ggcaatcgtg aacagccagg tggacaaggc
    59281 ccccatctcc ggccggtaac ggtccagctg ctgccaggcg cgcacgaagg cgtcctgggc
    59341 cagatcctcg gcgagggccg gcgcgcaccc catgcggccg agaaagccga agaggggccg
    59401 ctggtagcgc accaccagcg cggcaaaggc ctggcggtcg ccggaacgga ggcgggcaag
    59461 cagatcaggc gtgtccatgg gtgagtccgg ggtgcatgcc gcttataccg gacccacgga
    59521 aaaaggtgac aggccgaatg tgcggccggc acgccgatac accccggccc cccggggcgc
    59581 ctgtccgtct ccggacgatc cgcgcacgca ttccggcgca ggcatccgcg cccggcggaa
    59641 ttgcgtaaac ccgatgaagg cccccgccgg cccggacctt cggccgacgc gggcgcgccg
    59701 tcccccgccc gccgaccgtg aatatgtcac accgtgtgtc gtaaaccgcg ccggaggccg
    59761 atgcgcaacg cccacgggac agcatcctgc aaggctggcc tgctaacgta tataaaaagt
    59821 tatagaggtt cctgcaacat gctcactcaa ccgatccgct ccaagcaggc cgtggccgcc
    59881 gagcgcgaat gggccggcca ggcgcggcgg tcctccacgc ccgaccagca cgccgccccg
    59941 ccgacgccca gcgccggccg gggaaagctg tggctcctgc tcctcgccgt cgcccttatc
    60001 gcgcccaagc tcctgcacta cttctaagcg cggcaacggc cgcccgtccg ccgggcggcc
    60061 agacgcggcc gcggaacgac acctatttca gatcgaagcg gtccaggtcc atcaccttgc
    60121 cccaggccgc cgcaaagtcg cccgcaaacc tcgcggccgc atcggcctgg gcgtacacct
    60181 cggcaatcgc ccgcagctgt gaattggagc cgaagaccag atccacccgc gtcgccgtcc
    60241 agcggagcgc gccgctgtgg cggtcgcggc cggtaaaagt ctcccccgtc tccgcggtgg
    60301 gtgcccagac ggtgcccatg tccagcagat tcacaaagaa gtcgttgctg agcacccccg
    60361 gccgcgacgt gaacacccca tggggtgcgc cccccacgtt ggcccccagc acccgcagac
    60421 cgcccaccag caccgtcatc tccggcgcac tgagggtcag cagctgggcc ttgtccacca
    60481 gcagggcctc ggccggcacg cggaagctct gcgcgaggta attgcggaag ccgtccgcct
    60541 ggggctccag cacggcaaag gactccacgt cggtctgatc cgcgcgcgca tccacgcgcc
    60601 ccggcgcgaa gggcacgctc accgtgacgc cgccggcctg cgccgccgct tccaccgctg
    60661 cattgcccgc cagcacgatc agatccgcca gggacacctg gcgcccgtcg gtctgcgcgg
    60721 cgttgaagtc ccgctggatg ccttccagcg tcgccagcac gcgggcgagc tgcgggggct
    60781 gattgaccgc ccagtccttc tggggcgcca ggcggatacg cgcgccgttg gccccgccgc
    60841 gcaggtccga accgcggaag gtgctggccg atgcccacgc ggtgctcacc agctcggcga
    60901 cgcccagccc ggcctgcagc acccgcgcct tcagaccggc gatgtcggcc gcgtcgatca
    60961 gcgggtggtc ccgcgccggc agcgggtcct gccagatcag gtcctccgcc ggcacctccg
    61021 ggcccaggta acgggccctg ggccccatgt cccggtgggt cagcttgaac caggcccgcg
    61081 caaaggcgtc ggcaaaggcc gccggatcct gctggaagcg ccgcgcaatg ggcgcgtaga
    61141 tcgggtcgaa gcgcagggac aggtccgccg tggtcatcat cggcggatgc ttcttcgccg
    61201 gatcgtgggc gtccgggatc aggtgctccg gcttcacgtc ctgcgccacc cactgctgcg
    61261 cgccggccgg gctccgggtc agcgcccact cgtagccgaa gagcatgtcg aagtagccgc
    61321 cgtcccaggt ggtcgggttc ggcttccagg ccccctcaat gccgctggtg gtggtgtcgc
    61381 cgcccgtgcc gcggccgaag gcattgatcc agcccaggcc ctgggcttcc aggggcgcgc
    61441 cttccggttc cggccccacc catttcggat caccggcccc gtgggccttg ccgaaggtgt
    61501 gaccgcccgc caccagggcc acggtctcct cgtcattcat ggccatgcgc gcaaaggtct
    61561 cacgcacatc gcggcccgag gccaccgggt ccggctggcc gtccgggcct tccgggttca
    61621 cgtagatcag gcccatctgc accgccgcca gcgggttttc cagatcgcgg gggccgctgt
    61681 agcggctctc cggctggtcg ctggtggcca gccaggtctt ctcggcgccc cagtagatgt
    61741 cctcctccgg cgcccagatg tcggcccgcc cgccgccgaa gccgaaggtc ttgaagccca
    61801 tggactccag cgccacgttg ccggccagga tcatcaggtc tgcccaggag atgcggttgc
    61861 cgtacttctg cttgatcggc cacagcagac gccgcgcctt gtccaggttg ccgttgtccg
    61921 gccagctgtt gaggggcgcg aagcgctggt tgccgctgcc cgcgccgccg cggccgtccg
    61981 ccgtgcgata ggtgccggcg ctgtgccagg ccatgcggat gaagagcccg ccgtagtggc
    62041 cccagtccgc cggccaccag tcctgggaat ccgtcatcag ggcgtgcagg tcggccttca
    62101 gggccgcata gtccagttgc cggaaggccg cggcgtaatc gaagtccgcc cccaggggat
    62161 tcgacgccgg cccgtgctgg tgcaggatcg acaggttcaa ctggttcggc caccagtccc
    62221 ggttgctgcg cgcgccggtg gtggcgcggg tgccgtgggc ggcatggaag gggcattggg
    62281 atgcggtgct catgggggtc tcctcgtgtg gtctcacgcc tgatgcggct gccttgaggc
    62341 tagtcagccg gcgccgccga atccacacaa accttccaat cgtccgcatc gccccaggct
    62401 atcggcccgc agcatcccga ccagacgtgt ggaaagcccg cttccgcggc ggaaagccgc
    62461 ctgccatccc gcgccgccgc tcatccttgc gcgggcccgc aagtccttga gcaaccgcgc
    62521 ccgccaggcc tgctgcccat ggcacggaag ttgtatgaac aatctacggt tctgcttaca
    62581 ctgccctggt cccgatgccg cccaccgtcg ctccacccgt gcccccgacc cgaccgcccg
    62641 cggccacctc tcctggcaag gacgcacgcc cccgcacacc caccccggcc gtcggcagcg
    62701 ccctgcccgc ccgccgcctc gccctgtgtg cctgcctggg gctgggcgcc gccatcgccc
    62761 acgccgacgg cgtgcagacc ctcgacgtgg tggaagtcac cgccagcgcc accgacctgg
    62821 tcggcgtggc gggcgccgcc agcgaaggca cggtcacggc aaaacacctg gccaaccgcc
    62881 ccctgctgcg ccccgccgag gtgctggagg ccgtccccgg cctggtcatc tcccagcaca
    62941 gcggcgacgg caaggccaac cagtactacc tgcgcggctt caacctggac cacggcaccg
    63001 acttcgccac caccctgctg ggcatgccca tcaacctgcc cacccacgcc cacggccagg
    63061 gctattccga cctccagttc ctgatccccg aactcgtgga cacggtgcgc taccgcaaag
    63121 gcccctacgc cgccgacgtg ggcgacttcg gctccgccgg ttccgccgcc atcgactacg
    63181 tccgccgcct gccacaggcc ttcgtcgaac tcaccgccgg gtcctacggc taccgccgcg
    63241 gcctcgtcgc cggctcgccc gcggtggccg acggccacct gctggccgcc ttcgaatgga
    63301 ccggccacga cggcccctgg gccgtcccgg aagatctgcg caagctcaac ggcctgctcc
    63361 gctacagcga cggcacccgc agcaacggct ggtccgccac cgccctggcc tactccgccc
    63421 gctggaacgc caccgaccag gtccccttgc gcgccgtgca gtccggcctc atcggccgct
    63481 acggcagcct cgaccccaca gatggcggca ccacccagcg caccagcctg tccgtcgaag
    63541 cctcgcgcca ggacgccgat ggctggctgc gcggcaacgc ctacctcatc gactaccgcc
    63601 tccagctgtg gtccaacttc acctcctgca ccctcggctg cgcccccggc cccggcgacc
    63661 agttcgaaca ggaagaccgg cgcaggatcg gcggcttcaa cctcgcccac acctggtacc
    63721 tcaacagcct cgcccgcggc gccgacttca ccctgggcgt gcagagccgc gtcgacgaca
    63781 tcggccgcgt cggcctctat accaccaccg cccgccagcg ctggggcagc atcgcccgcg
    63841 acagcgtccg gcaaggcagc ctcggcctct acggcgaagc caccgtccag tggctcgaaa
    63901 aactccgcag cgtcgccggc ctgcgggccg accgctaccg cttcgacgtc accgccgaca
    63961 ccccggccaa ttccggccag gtggacgccg ccctcgccag ccccaagctg gccctcatcc
    64021 tcggtccctg ggcccgtacc gaggtctatc tcaacttcgg ccacggcttc cacagcaacg
    64081 acgcccgcgg catcaccctc accacccagc cggacttccg cgaccccgcc ttcggcacgc
    64141 ccgcccacgc ctccaccccc ctggtgcgca caaagggcag cgaaatcggc gtgcgcagcg
    64201 ccctcctccc cggcctgcac agcagcctcg ccctgtggca gctcgacatc gcctccgagc
    64261 tcgtcttcac cggcgacgcc ggcaccaccg aagcccgctt ccccagccgc cgccgcggga
    64321 tcgaatgggc caacgattgg accccgctca ccggcctcat cgtggacgcc gacttctccg
    64381 tctcccgcgc ccgctacacc gccgtggacg ccggcgtgcc cggcgaccgc atccccggcg
    64441 ccctcgaaca ggtcgcctct gtcggcgtcg cctgggatgc gggcggcccc tggtccggcg
    64501 gcgcccgcct gcgctacgtc ggcccccgcc ccctcatcga ggacaacacc gtccgcgccc
    64561 ccggctccac cctcgtcaac ctgcgcgcca gccaccgcat cgacccgcgc acgcggatca
    64621 gcgtagacct cctcaacgcc ttcaaccgca aggtcagcga catcgactac tactacgcct
    64681 cgcaactgcg cggcgaagcc gccccggtgg acgacctcca cacccacccc gccgaaccgc
    64741 gcagcctgcg ggtgagtctg cgggtcgggt tct
//
LOCUS       DAOQMM010000008        60551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_83_length_60551_cov_45.776514, whole genome shotgun sequence.
ACCESSION   DAOQMM010000008 DAOQMM010000000
VERSION     DAOQMM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 60551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60551
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_60551_cov_45.776514"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PLH85_02490"
     CDS             <1..1485
                     /locus_tag="PLH85_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63455.1"
                     /translation="LVLDPTCGSGTTAFVAEKWGRRWITCDTSRVAVTLAKQRLMTAS
                     YDYFDLKYPHEGLKGGFIYKTVPHVTLKSIANNPEIDEIHARLHPAVATALARLNTAL
                     ADGGVADFVVTEGGRKGRALRLTAAEALLEWEVPFEPPADWPAAARAAHGDFLEARRS
                     LQTQMDRSIAAHADPEVLYDQPAVDKNRLRITGPFTVEAVPFPSVKGLDEAQGPEEAD
                     ASIARTGESGRQHQWRDELLKTGIRGKGGQRLKFVDLEALPSDRDIRSLHATGHLDSG
                     ERVVVSFGPEYAALEQRQVANALNEAGQLFPLPRMIVFCAFTFDPEAAKDIDGMKGIV
                     ALKAQMNTDLLTEDLKKARSGNQSFWLMGQPEVEVRRLKDGRYQVEVHGFDYFDTVKG
                     ELVSGGKSRIALWSLDTDYDDRSLFPRQVFFPMAGKGEGWEKLKKNIRAELDPARLDA
                     FHGTVSLPFAAGDHGKIAVKIVDDRGIESLKVMTLEDRKEGHPQ"
     gene            1482..2699
                     /locus_tag="PLH85_02495"
     CDS             1482..2699
                     /locus_tag="PLH85_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB63456.1"
                     /translation="MSTHSQGRLIMENVGPIVAADVSFGDLTVIVGPQATGKSIFLQT
                     LKLLLDRDHIHDTFLHNSIGFSGDSAAFLGGYFGKGMAGMWRDGDSTLKWNGKSSPLT
                     DLARPSKSRNRQERLFYIPAQRVMSLVNGTTQNFGQFNYGDPYALRYFSDAVHGLVQN
                     EFGPRGKLFPLPNRLNAVLRKPIEQHLFGGSHLATDAKDFTKRLVLNVAGHDEGLPYT
                     AWSAGQREFTPLLLGLYWLCPAGKVTRREPIEWVVIEEPEMGLHPHAILTILLLVQEL
                     LQRGYKVVLSTHSPVVLDWVWALQEFKRHGAGEGDVRRLMKLHANPFSKKLAQAALEK
                     TYKVYFFDRMAEAKDISDLDPNAESLDEAQWGGLIGFASHVGELIADVVNRAPATEPT
                     PQDAAADDEAEDA"
     gene            2696..3229
                     /locus_tag="PLH85_02500"
     CDS             2696..3229
                     /locus_tag="PLH85_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63457.1"
                     /translation="MSPAHTQPKKAGQHAARPDTAFVHAVKAAGLTPAAGKGAVEGKY
                     SASIVATKEGAVFTGSVDLDTQFKAAESRSNRWDYGVGIKDRGQPECAIWIEPHSGTS
                     AGEVAVILAKLKWLQAKLESPTFHPLKVLTAEAIRQGKTPYVWLASGKVGIRAGSREA
                     RQLASAGMRLPMSQARI"
     gene            3394..4554
                     /locus_tag="PLH85_02505"
     CDS             3394..4554
                     /locus_tag="PLH85_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB63458.1"
                     /translation="MQIVRLKLKNWRNFREVDIPLGPRTVVIGANASGKSNLLDVFRF
                     LRTVAQKDGGGVQKALRERDGLVKLRSLHARRDPEVRIEVELRAAPPEEAHVWRYVLA
                     LRSEGTGQQRVLVAEERVERDGVPILERPDAADRKDKERLTQTHLEQIGSNGDFRALA
                     ESFAATTYLHLVPQLLKHGDEIATRIPENDPFGQGLLQRIAKTPRQTRDARLKRIQKA
                     LADAVPLFSELRFEQDAVTGLWHLEARFTHWRTHGAWQRENQFSDGTLRLIGLLWALQ
                     EGDGLLLLEEPELSLNDGIVARVPLLIDRVLRSRKRAATSRQVLVSTHSETLLAHSQG
                     DALLLIEPSANGSTIRPPTAEEAEQMQHGLNPAEVLLPKTRPDSLDQLGLWE"
     gene            4551..5153
                     /locus_tag="PLH85_02510"
     CDS             4551..5153
                     /locus_tag="PLH85_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63459.1"
                     /translation="MSRILVVGEDPLCAALGERLIASALPSWQLAGPSIPSRSFSDLV
                     PKLSRYAGLARDLHPVLCIADSDRKCPVELVRDHRPMWAPDRFVFRLAVSEAESWVLA
                     DRECFAAAFEVPLARVPRNVDGLADVKEQLLSLVRASRVRRYREEIVSESSCPRPGHG
                     YNLHLCAFVREQWSAQRARHASPSLDRAVSRLQALGEAPA"
     gene            5164..8031
                     /locus_tag="PLH85_02515"
     CDS             5164..8031
                     /locus_tag="PLH85_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNB63460.1"
                     /translation="MHSLIISSPFERPTHHWRRVNDNRLELVPGRRSAGYEVFDTRHN
                     TVRAVELSLVNRIRARVDAWRAADYPGVTTVTRQLLAHWCDSENSPRQHAFYFCQIEA
                     VETLIWHVEAPPDFKQGIAVPGDGGPWERLCSKMATGTGKTTVMAMIITWQVLNALSY
                     PKRGKDFSRAVFVVAPGLTVKERLQVLRPGHPDNYYDLFHLCPNEGMRQKLNQMALGI
                     ENWHHLMPLKEPERSVVKKGAESDEAYVRRVLGPVAASRDIVILNDEAHHAYRQRAEV
                     KVSRKDAEELGIDLDEATRWIEGLDRIHKMRRIARCFDLSATPFAPTGKTSTEQALFE
                     WVVSDFGLNDAIEAGLVKTPRVVVRDNALPNAQTYRPKLYHLYREREVAEDLNRRGAQ
                     AHEALPQIVQEAYTLLGADWRETLLAWQASGHTSPPVMLTVCNRTETAARIEHYFRHG
                     DAYWPELKAPEKTLRVDSRVLEKAEMGESSGSDKAYEAVLHEILEAAAIPEARKAELR
                     ELKKEELLRAVVDNVGKRNTAGQDLQNVISVAMLSEGWDAKNVTHIMGLRAFTSQLLC
                     EQVIGRGLRRVTYETERVRCPDGVERDLFQPEYVNVFGVPLSIFQDSDGVGAAPPPPK
                     HSTQVESLKDRGELEISWPNVLRVDQVLRAELHADWSLMPALKLDPAQIHVSADLAPA
                     LGAAADLAKAVSIDLEKLPEEFRLQRLLFVAARKAFETVRHGFTGSRESLIHQLIGLV
                     EDFFASDRLVIPSAFHQDTLRKRILIALSMDAIVQHVIEHVHQHHAARLEPVFDPEFP
                     IGSTRFMRTWYTTKPCIETTRSQISHAVSDSTWEHYTTQVLERRSDVAAYAKNDHLGF
                     QLYYLWRGSRRKYVPDYLIRLGNGKHLVLEIKGEDSDQNRCKRLALDAWVRAVNTRGG
                     FGVWCWDVVKGEPARVDDVVGHHAQRGTMTPPIGENLDPVH"
     gene            complement(8181..8567)
                     /locus_tag="PLH85_02520"
     CDS             complement(8181..8567)
                     /locus_tag="PLH85_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNB63461.1"
                     /translation="MGLTPIRLLLFAMIVLALQYGWKHMPHPGAGGSPTAEALRSIAS
                     TVQPGEVTMYSTTECAYCAQARAWLTQYGFAFTECNMSLDRRCEREFLAYGATGTPYL
                     VIRRGGRTYHMKDGFDSEEFLRVLKG"
     gene            complement(8718..10703)
                     /locus_tag="PLH85_02525"
     CDS             complement(8718..10703)
                     /locus_tag="PLH85_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNB63462.1"
                     /translation="MSKLTRVGWYWNRLRCMSVAEMVERASTSAGHALERRRRPVPIP
                     PPDLTRRGACWLPAHELPPVPPAVIDRADEILGGRWRIFARGPVALGLPPEWNRNPHN
                     GAVLPMAFGKTMAFRDTTVIGDIKYLWEPNRHLELVVLAQAWKATGLRKYLDALGTLL
                     TSWFRQCPYPLGPNWSSALEAGIRLINWSIVWQLIGGFESPLFAPPEGVVLLRDWLDS
                     IWRHAEFIRGHRSLHSSANNHLIGELSGLYVASVTWPCWEDAPRWREAARAGLETEVL
                     RQNAPDGVNLEQATSYQQFVWDFLLFAGLAARATGDDFSTAYWQRMEAMLDYVAAIMD
                     TGGQVPQFGDADDGLACGLSLCADRDPFRSQLATGAVLFKRSAFARKVGRFDLRSVWL
                     LGRAAADDFATLLVDRSEPLTPRQAFPDGGVYVLGSALDTPREVRVVADAGPLGLGGI
                     AAHGHADALSFTLSMDGREFLVDPGTGTYHGPRAWRDGFRSTALHNTLTVDGEDQAIT
                     GGKFMWIRKYEVSVLEWSSSADADRLVAEHDGYRRLAGHPVHRRAWRLDKRSGTLSVR
                     DELGAPAPRPVALNWHFAENCVITCTPDGLVVGHDGVTLLVTLPPGGETTVICGSDVP
                     LCGWVSRGYDLRRASTTVIWRGHLGPGQAAETVFRRG"
     gene            complement(10700..12094)
                     /locus_tag="PLH85_02530"
     CDS             complement(10700..12094)
                     /locus_tag="PLH85_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HNB63463.1"
                     /translation="MIVSCRSPLYQLAPVALQERLLSWQTRLRRWLTRPAALAQTRHA
                     IADSQWLSREGWQSLQFAAVRDLALHAARHTRYYQDVFACLGTDPSAWTSLDDLSRVP
                     ALSKDEVISIGDGLLARDGTWLRFQGSTSGTTGRAMRGWRDQASVTFEQAFVDRQAEW
                     AGYRPGDRRAWLRGDPIVPTRQQGGPLWRFNRADNMLMLSSYHLSRYNGLAYLHALES
                     FDPVLLQAYPSSVAYLARLLEDEGRHYRGPALRGIVTSSETLGADDRRVIAERFGCPV
                     FDWYGAFERVAAIGTCEHGRYHVMEDYGVTEFEDNGDGTANLIGTGFGNRSMPLLRYR
                     SDDRVVLADPDFQCGCGRSFRVVERILGRVDDVIRTPDGRHVVMLDWIFAGLFGLIEA
                     QVVQERLDLVRIRIVVAPEFSAQDEQHLLQRARERLGPQVTLRIERVPAIERTRNGKF
                     RQIVSRIHSRQDIR"
     gene            complement(12091..13182)
                     /locus_tag="PLH85_02535"
     CDS             complement(12091..13182)
                     /locus_tag="PLH85_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB63464.1"
                     /translation="MSKKAPALIMLGPDPEARGGIASVQRIWLQAGLLDERPVRVLTT
                     YRDGSALRKLGAFVTALLRFLLLLAAGRVAAVHVHSASNASFRRKSVFLALARLAGKP
                     YVFHLHGGAFDHYYAGASFLERAWVDRTLHGAAVVFTLSEGWANWLRINVGHPDVRVL
                     PNPVLPVTLPAGVAREPATLLFLGRLEDAKGVFVLVKAIARLARKFPDLRAVIAGDGD
                     IRAVRDAAERAGVLDRIETPGWVDGGKKLRLLARAGVFVLPSRFEGLPMSVLEALAAG
                     LPVVASRVGGIPQVVRNGDNGALHEADDVASLASALERVLADPAAARAMGERGRRRVL
                     ADYGVERIRALLNAVWDSLMTPASERFAR"
     gene            complement(13175..14608)
                     /locus_tag="PLH85_02540"
     CDS             complement(13175..14608)
                     /locus_tag="PLH85_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63465.1"
                     /translation="MGLEDWGYRWLKRYMDAPQWIKEPLGRVYGLLPDSIRHGKDFGR
                     YRDEAGLTDAEALRRLRDARLRETLLWAAQTVPAYAAIAAQLLTDLPAEEWLSLFPLM
                     TKLDYRRNPEHYLSTGCSPAERLPMQTSGSVAEPFRFYLHKGVSRAKETAYIESYGRR
                     IGLTRKDVVLSLRGRNVRGSEADGGPLWSYEPIKRMLWISPNHLDGSYMPRHVQAAIE
                     HKVSFIQGYSSAVYELARWLDFNPVPRFSDAIRGVQLFSECAEPDMIALIEKVFGCPV
                     LIHYGHSERSVMAGSMPDDHRYFVWPLYGHIELIDEAGRPITEPGVQGEVVATGFDNR
                     VMPFIRYRTGDLAAWSDGPAHPDLPGYPVLERIDGRNNDFFVGRDGRRIPVASLGGLR
                     PPEFLQVEASQYEQFEPGRLIVRLQSPSPLPADTVAVLDNYFRVKLLGCVEPSLEVVP
                     HIARTARGKRRLMIQHVTSEVEEGSRV"
     gene            complement(14625..15344)
                     /locus_tag="PLH85_02545"
     CDS             complement(14625..15344)
                     /locus_tag="PLH85_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNB63466.1"
                     /translation="MMGCWVDNLTMEETLGTVENFIHSGRPHQHVVVNVDKIVKASRD
                     PVLRNIINGCDLINADGMPVVWASRLLGKPLKERVTGVDLFESLMERSAEKGWRVFLL
                     GAREEVVSRVAQIYPARYPGLTIAGYRNGYWKPEDEAGVANAIAATRPDILFVAISSP
                     KKENFLARYQAAMKIPFAMGVGGTFDVAVGLVRRAPVWMQKAGLEWFYRFLQEPRRMF
                     RRYFIDDMAFVALFAREWMKR"
     gene            complement(15353..16954)
                     /locus_tag="PLH85_02550"
     CDS             complement(15353..16954)
                     /locus_tag="PLH85_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63467.1"
                     /translation="MNVLRLSIFLPDGPVPGWVVRLLDVCGAEPGVILVGVLYDAQPM
                     AAADWAEQVDRLLFDRGASSLDPVSLELPGLLPVWRAEAGVAPARWLAERACDVLLDL
                     RPRPTPIPQLAAEHWMLSIAGRPATDAGAGAQPVVGGDATTRVVLERVGGGAILDTSV
                     GATCEFSIRRNRRRALAKGIGMVRRALRRMRTQVPPPPQRDTLPAAAVQPPGAVARVA
                     AVLGRTARRALRKGLSRDQWGIALAFGDDVCLDPARAQLILPPPDRFWADPMVWPAGQ
                     DGWWVFIEEVLYAEGRGTLQALRVHRDGRWEAPVPVMARPWHLSYPFLFWWDGALWMV
                     PESGANRRVELYRCTRLPDRWEKFTDLLTDIDLVDATLFAHDGRWWLLGNVSEPGADK
                     HDELHAFHAPSPLGPWTPHAANPVVADARVARPAGPVFERDGHLLRPAQVCVPEYGHA
                     LVLRRIEKLTTEEYVERTERRIDPGWLPGIACVHTLSHEAGLTAFDFLIRRSRLLPPP
                     AALRRAVLHGGGEERCDMPRAEVPR"
     gene            complement(16951..18192)
                     /locus_tag="PLH85_02555"
     CDS             complement(16951..18192)
                     /locus_tag="PLH85_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63468.1"
                     /translation="MSRDLTALVRRVEVLAVTAAPGGQRLLHFMLVQMACGTEALGRF
                     ANDLAIAMMLGLFSAVGWSALVLARVPVAPAGRSAPVMLGLARGVAVFTALACLPLPL
                     LAWGGWVFEPGWTALLLAGWTAYTVARHYGLALGAYRVLLVVETLVVALLAAGLLIFG
                     RAHPVMAWAAFAVPTALLGALAVLVLIRRARDQGGAAALPAGSARTAAEQAALNFVSG
                     GMSMILVPCTVQVAGEAYGGLVALIGSVTSVLLLFPRALSFNALPALARVVRQQGAAD
                     VRALLDALRRTLFRVNSGLTLVAVAAWGLMAGVGERPAFALAGATAIFLLQLASFYVG
                     QLVLPEANYLQTVEETRLPLVVNLASLGLFVVACGGVMLLVPKGVDAVLGLFAVLLGA
                     TFARNLLLRRAVLHRAPEVFA"
     gene            complement(18189..19424)
                     /locus_tag="PLH85_02560"
     CDS             complement(18189..19424)
                     /locus_tag="PLH85_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63469.1"
                     /translation="MRFALPAFDVAELTAFHLENRVARALTWAMFVGLWAKLAVSVLL
                     VQPVIDAEADSFYSRAADAMLLCAIFVGLATYADELLRRAPLVLAGVPLVLASFMTTE
                     ILTRDGFTLATFEYLKIVTPLLMSAVLLRLFEREPALVVRLMQFTLAIVWLGVIGALL
                     FLPPSLNRGGEPFWPVYFASLHTSSYLVVVCAGLSLYLYEADHFGKRLFIAQILFSFV
                     LVFHGWGVRTSMVAAVAFGLVYFFQHSRKAALVVFLTIVAVLTLAYLPLLFGVVHLPS
                     WDEMVELSSGRMSMWLQKADILANSTLLEWLFGHGEGSDWIVSEIWWWSAKDSHNDLI
                     RLLNQQGLLGLGGVLWLLWVWARLFPAGVAAPLLAALLASTAFSNGIMFRPQAGLLMP
                     LALVAAADAWQRRQAGRAA"
     gene            complement(19424..20314)
                     /locus_tag="PLH85_02565"
     CDS             complement(19424..20314)
                     /locus_tag="PLH85_02565"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNB63470.1"
                     /translation="MDKAKFSGFGNVAPLERARDATPSLGELLVSAGRLSADAASRVA
                     TRQRERGIPFGQAAVELGLVSEADVRAALARQFDYPVLAAGDPSVAPELVAAFTPDAP
                     QVEALRKLRSQLVLRRAGNPHCKTVAVVGAGRGEGRSWLVANLAITFSQLGERTLVMD
                     ADLRTPRQQALFRLPPAGGLAAHLAERGDSQPPVVHPRFPGLAIQPAGNVPPNPQELL
                     ARSGFAAHVTAARDFYDMVLVDTPADEVGADAQLVAAACGFAVVVARRHITAHKRAGA
                     LAASLRDAGVTILGGVLLEF"
     gene            complement(20324..21703)
                     /gene="epsF"
                     /locus_tag="PLH85_02570"
     CDS             complement(20324..21703)
                     /gene="epsF"
                     /locus_tag="PLH85_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HNB63471.1"
                     /translation="MGITQLLAVLKARRTLLFGVWGGVVLLALLVSLILPRQYAATAE
                     VVVESRGSDPLAPPQGVGLTTPGFLVTQADIIASERVGARAVAALKLMDQPRWAERWQ
                     KASGGQGDPARWIAGKLKKTVKVSPSHESNVLTITVTADDPAFAANYANALAKAYLET
                     DLELKIEPAREYATWFDEHTKVLRANLEQAQSRLSAFQREHGLVGDEKLDLESARLGE
                     LSSQFTAVQAQSTEATERQRQGGGANEVASNPLIQALKVDLARQEAKLTELSGYLGEN
                     HPQYQRTKSEVESLRARLRQETGNVAAMVGTNSRISQAREGDLKGRLDAQKQRVLALK
                     QQRDEMMLLQREVDSAQKAYDLVMARLTQTSLESKARQGNVALLSSAVAPTEASSPKV
                     LLNVGLALVIGALLGLAVTIVRELFDRRVRAAGDLADALGVPLVVEVKAAPAAAAGAR
                     PFWPRLRRA"
     gene            complement(21717..22562)
                     /gene="epsE"
                     /locus_tag="PLH85_02575"
     CDS             complement(21717..22562)
                     /gene="epsE"
                     /locus_tag="PLH85_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HNB63472.1"
                     /translation="MGILKQGREGWQRWLLCVVAMLAAALTGSLALAQGPAPAAQEYR
                     LGPGDGVKISVFQNPDLGLETRVSETGVISYPLVGSVQVGGLTVGEAEKAIAAGLRSG
                     GYVLQPQVTLVVQQVRGAQVAVLGQVARPGRYPLESTAMKVTDALALAGGTTPQGAEK
                     VILTGLRNGRRVRLEVDVADLFVTWTPEKDVPVQAGDTLYVQRAPQFYVYGEVLKAGA
                     YRVERNMTVLQALSLGGGLNPRGTERGIRIHRRGDDGSVRVINPQLNDPVQADDVIVV
                     REALF"
     gene            complement(22550..23890)
                     /locus_tag="PLH85_02580"
     CDS             complement(22550..23890)
                     /locus_tag="PLH85_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63473.1"
                     /translation="MLGVGSPVLALDASALRVSTLLGEASRQAAPLFESDELDPPALT
                     LTGTLGPEAAGLPERGGEPLDLPVGKSAGTRISVAENLTYDSNVFRLRDSAAAVAAGL
                     AHLGSWQSQTKLGLGLDKSYAGQQLLLDYDATMARYSHFGFLDHTVQGGIGRLRWKAG
                     QDLKGELVSEYREALDDYAYYRSPLRSISTTRQMGGNAFYTLVGGWQIGAQAGRGSRR
                     YKDGTRPTNEIDFAGVDGVLRYVPERGGAVALTRRRARGEYPNLPATAGSLAETRFNQ
                     DETFLEVSLPVRSGSRYLMRVGRVSRNYERYPQSNFSGRNGLIGAEWQVTPRTQLNGS
                     LRYDVEPAQDFVSSYVATKGARLGAVWEYSPKTRIESRYEWRNAQYRGDPGFGVVAAS
                     RREDTATLVGVTALHAMSARTRWTASLSRERRDSNQQEWSFTDWIVAGSLQWVF"
     assembly_gap    24025..24124
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24125..25046
                     /locus_tag="PLH85_02585"
     CDS             <24125..25046
                     /locus_tag="PLH85_02585"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNB63474.1"
                     /translation="RMGASRREGKQLLGFFDDRHAERLASPRLRLLGRIADLADYVRQ
                     QGVDVIYIALPMTTQPRIVRLLDDLRDTTVSVYFVPDLFVTDLIQARVDHVGGMPVVA
                     VCETPFTGLNGVLKQSFDFLMAACILILIAPLMLVIAAAVKCSSPGPALFRQYRYGLD
                     GERILVYKFRSMTVCENGETIAQARQNDARVTPLGAFLRKTSLDELPQFINVLQGRMS
                     IVGPRPHAVAHNEMYRKLIKGYMIRHKVKPGITGWAQVNGCRGETETLEKMKQRVDFD
                     LEYLRRWSLALDLRIILRTVRLIRKDPNAY"
     gene            complement(25164..25240)
                     /locus_tag="PLH85_02590"
     tRNA            complement(25164..25240)
                     /locus_tag="PLH85_02590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25204..25206),aa:Arg,seq:acg)
     gene            complement(25322..25398)
                     /locus_tag="PLH85_02595"
     tRNA            complement(25322..25398)
                     /locus_tag="PLH85_02595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25362..25364),aa:Arg,seq:acg)
     gene            25566..26345
                     /locus_tag="PLH85_02600"
     CDS             25566..26345
                     /locus_tag="PLH85_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB63475.1"
                     /translation="MHTLALRGHTLEVVRRPSAHPRPEAPALVFLHEGLGSIAMWRDF
                     PQRVADATGCEAIVYSRHGYGRSSPLTAPRTPDYMHVEALQILPAVLDALQLRAPILV
                     GHSDGGSIALIHAGGTPAPVRGVVVMAPHLYVEDVALEGIRRTVDAYRTTDLRARLAR
                     FHADVDGVFAGWSDIWLHPDFRDWNIEAFVSAIPCPVMAIQGEDDEYATMDQIDRIAR
                     LAPDVDLVKLADCRHSPHKDQADAVVEAIAGFVARLLDDPH"
     gene            26375..27349
                     /locus_tag="PLH85_02605"
     CDS             26375..27349
                     /locus_tag="PLH85_02605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HNB63476.1"
                     /translation="MRAYLCQAFGPLDQHGPGQIGDPQPADTEIVIDVAAAGVNYYDT
                     LIVQGRYQIRPPLPFSPGGEFAGTVCAVGAQVHEFTVGQRVLAFNSFGGYAEKARARA
                     AQVFPLPDAVSFESAAAALVTYATAWFALHDRGRMQPGETVLVLGAAGGVGLAAIQIA
                     RAAGCTVIAAASSREKLDLCAAAGAHTGIDYHANDLKDAVKAATGGRGADIVVDVVGD
                     RYTEAAVRATAWRGRVLIIGFAAGEIPRVPANLLLLKGCEMAGVFWDEFLRREPAECR
                     RQVAAILDGIARGIFQPPIHGRYGIEEAGQALTLLADRQATGKLLIVP"
     gene            complement(27442..28344)
                     /locus_tag="PLH85_02610"
     CDS             complement(27442..28344)
                     /locus_tag="PLH85_02610"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNB63477.1"
                     /translation="MLGAADSARDEVHSSAMRRWGRIFGVVAGGALGVLAVPVIVSQT
                     SLSGEALTETARGVGWGALVLAAGLAGRWAQGRRAHRVAQTEGAAAVATAPSGSQPRR
                     ARAWSVDVLQQLDRARFQALCCAFFRESGVAATTHAGDEDSSAGDIRLHQDPRRPGAV
                     TAVVRCVPWGPTIDVWAVRALAETRRRESADRAFFMAPGGFAPDALAFAAQGRMTLFD
                     SRLMLALIRRLPEDSQQRLLDLAATGDWTAPACPECGERMVVRNVIRRPFWGCSRFPQ
                     CRGTLPMNRKAGLQGDDGPLAMAG"
     gene            28534..29013
                     /locus_tag="PLH85_02615"
     CDS             28534..29013
                     /locus_tag="PLH85_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HNB63478.1"
                     /translation="MNDVLHAAVTDALARHGHRPDALLPLFHALQDRIGWVPPDAVPM
                     LATALNLSRAEVHGVLSFYPHFRRTRPGRHVLQICRAESCQARGGEALEAHARSLLGT
                     GWHGTSADGAVTLEPVFCLGNCACSPAVRIGDELLGRMDVARLEALIARLRTSEPAP"
     gene            29010..30557
                     /locus_tag="PLH85_02620"
     CDS             29010..30557
                     /locus_tag="PLH85_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNB63479.1"
                     /translation="MNVRLFVPADSGALSVGADDVARALVREAASRGVTVELVRNGSR
                     GLYWLEPLVEVDTPAGRLAYGPVSVEDVPGLFDAGFLTGGMHRLGHGLTEHIPFLAGQ
                     ERLTFRRVGVIDPRSLADYQNHGGFDGLRRALALGDAAVVREVTDSGLRGRGGAAFPT
                     GIKWATVQTAGDGDKYVVCNADEGDSGTFSDRMLMEGDPFCLVEGMTIAGLAVGARRG
                     YIYLRAEYPHAFRVLEAALATARSAGWLGDDVAGSGQAFDIELRLGAGAYICGEETAL
                     LESLEGRRGIVRAKPPLPALSGLFGRPTVINNVMSFAAVPLILARGAAFYRDYGMGRS
                     HGTLPFQLAGNLRHPGLVEKAFGITLRELLLDYGGGSASGRPIRAVQVGGPLGAYIPE
                     SRFDVPLDYETYAAMGAMIGHGGIVAFDDRADLGRLARYALEFCALESCGKCTPCRIG
                     SVRGVELIDRLLAGTDRPRDRALLDSLCDTMIHGSQCALGGMAPLPVQSLLTHFPDAF
                     GLAPTAA"
     gene            30570..33425
                     /gene="fdhF"
                     /locus_tag="PLH85_02625"
     CDS             30570..33425
                     /gene="fdhF"
                     /locus_tag="PLH85_02625"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNB63480.1"
                     /translation="MSLPCPPDAGTPARDGEATVRLLIDGQPVCVPAGTSVMHAAHTA
                     GCAIPKLCATDRLEAFGACRLCLVEIDGRKGYPASCTTPVAPDMVVRTHTPRLAELRR
                     NVMELTISDHPLDCLTCAANGRCELQDMAGAVGLRAVRYGLDGANHLGDRADDSNPYF
                     RYDPSKCIVCNRCVRACEEIQGSFALSIVGRGFDSRVRAGQDESFMASECVSCGACVS
                     VCPTATLMEKSVIEHGQGERRVTTTCAYCGVGCSFDAEVKGNSVVRMEPTRDGGANQG
                     HACVKGRFAWGYATHPDRVTTPMIRPSIDAPWQKVSWEEAVTHAAAEFRRIQAEHGRG
                     AIGGITSSRCTNEETYLVQKLVRTAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQD
                     FASVLKADVVVVIGANPTDGHPVFGALLKRRLRQGARLIVVDPRAIDLVRSPHVEATH
                     HLQLLPGTNVAMINALAHTVVTEGLADEAYVQARCDLASWQAWRDFVARPEHAPEAVE
                     AVTGVPAEAIRAAARLYATAGNGAIYYGLGVTEHSQGSTMVMGIANLAMATGNVGRPG
                     VGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSDATVRGSFEAAWGVRLDPEPGLRIPN
                     MLEAALDGSFRGIYIQGEDIVQSDPNTQHVEAALAQMECVVVQDLFLNETAKFAHVFL
                     PGASFLEKDGTFTNAERRISRVRRVMPPLSGKADWEATLALAAALGHPMPYDHPSDIM
                     DEIARLTPSFSGVTYELLERRGSVQWPCNADAPEGTPTMHVDRFIRGQGRFMLTPYVP
                     TSERVTRRFPLLLTTGRILSQYNVGAQTRRTPNVAWHGEDRLEIHPTDAEDRGIRDGD
                     WVGIASRAGETVLRATLTERVQPGVVYTTFHFPESGANVITTDNSDWATNCPEYKVTA
                     VQVTLCAQPSDWQQRFALRHHRQRGLQATATGGIPHE"
     gene            33418..33645
                     /locus_tag="PLH85_02630"
     CDS             33418..33645
                     /locus_tag="PLH85_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HNB63481.1"
                     /translation="MNEAKLVNMANQIATAVAANAEPARAAIDVAEHLMRFWDPRMRA
                     GLIACAGGAAGLDPLVREAVARLRDQAQATA"
     gene            complement(33624..34382)
                     /locus_tag="PLH85_02635"
     CDS             complement(33624..34382)
                     /locus_tag="PLH85_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB63482.1"
                     /translation="MKTSPVELSAVLPGIRLFAGLDPRAVAELSRAGHVATLPKGRGI
                     YATGELPRALYYLLSGQVKVAVTSAEGGEKVIDIVHAPQAFGVAELFGQAPHVSSAET
                     VGASVVLVIGREGVMRAIEYDARLSMRLLQTVADRQTAIERDIAADCFQSGCEKVVDY
                     LLRAVAPAAGWGDSVSLDLEIPKYLLAARLGFTPETLSRIFRELSDEGLIRVHGRRVE
                     LLGSLLRRHGVVPAQVRPPRSPERGRSGQAVAWA"
     gene            34700..36583
                     /locus_tag="PLH85_02640"
     CDS             34700..36583
                     /locus_tag="PLH85_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB63483.1"
                     /translation="MRPPDAAAFVTARAPRRLSVVARLGALIAAIGALAVVSILASMT
                     VAELASGQARAINLAGSLRMQSYVIAATLADGEHGALLERALSGFDERYAAQALTTAL
                     PASAEASIRLAYDDVGLFWAQTFRPYAEAAPEPDRDARNRLTADLVARIDHLVALIEH
                     NLESRLQTLRLVQGISLFLLLVLGGVVVHQINRQVRAPLGELLTCAHVVRRGDFSVRA
                     APRPPDELGQLGEAFNYMVEDLSRSYSQLETRVEEKTRELARSNQSLELLYRTTRALS
                     EADLTQDSLLKVLNEVERLAGVRAGALCADPGDGRPLVVLATDMDTDLVARLPLDGPP
                     SAAPAALQVLPALSYAGDGALITVPVSDGVRSLGRLFLQLPDSAPPAEWQTPLLETVG
                     RHVGAALANARRHEERHRLALLDERAVIARELHDSLAQSLSYLKIQVTRLQNLLARQS
                     VSDPVGDVVRELRDGLNEAYRQLRELLTTFRLRIDGRGLTAAIDDSVREFSQRGGLAI
                     HLSNRIAGLELPAGREIHVLQIIREALSNIERHARAHTVDIVLERLDAGDLRVCIDDD
                     GVGIGEQPAPPRRFGIAIMHDRARSLDGALTVTARTDRGTRVELCFPAEPPAPPSPRG
                     ISP"
     gene            36580..37233
                     /gene="narL"
                     /locus_tag="PLH85_02645"
     CDS             36580..37233
                     /gene="narL"
                     /locus_tag="PLH85_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HNB63484.1"
                     /translation="MTATEAPSTVIVIDDHPLFRKGVCQLIALSDRLRLVGEASSGED
                     GLTLAQTADPDLILLDLNMKGMSGIDTLRALRDADLGARIIILTVSDAAEDLVAAIRA
                     GADGYLLKDTEPEDLLARMSEALAGRIVIDDSLTGLLARSLREEAQSAERSLAHLTER
                     ERAILQCLATGLSNKLIGRELGIAEGTVKVHIKSLLRKLKFRSRLEAAVWAIEQDTR"
     gene            complement(37752..37845)
                     /locus_tag="PLH85_02650"
     tRNA            complement(37752..37845)
                     /locus_tag="PLH85_02650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37809..37811),aa:Ser,seq:gct)
     gene            complement(37889..39118)
                     /locus_tag="PLH85_02655"
     CDS             complement(37889..39118)
                     /locus_tag="PLH85_02655"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNB63485.1"
                     /translation="MALIVQKYGGTSVGNPERIKNVAKRVAKFHAQGHQTVVVLSAMS
                     GETNRLIALAKEISGSPSPRELDVVVSTGEQVTIGLLCMALEEVGVPARSYTGGQVRI
                     LTDSSFTKARILDIDGDNMRRDLDAGRVVVVAGFQGVDAEGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVKLRVLSSFQEEGEGTLITVEEDKNMEQPIISGIAFNRDEAKLTVLGVPDKPG
                     IAYQILGPVADANVDVDMIIQNVGHDGTTDFSFTVNRSDYDKTVKILQDVQAHVGARE
                     IKGDRTMAKVSIVGVGMRSHPGVASRMFRTLAEEGINIQMISTSEIKISVAIDEKYLE
                     LAVRVLHRAFGLDQAAA"
     gene            39426..39728
                     /locus_tag="PLH85_02660"
     CDS             39426..39728
                     /locus_tag="PLH85_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB63486.1"
                     /translation="MKTNEKVDVREIRRKLGMNQSQFWSKIGVTQSGGSRYESGRNIP
                     RPVQALLRLVHIEQVDINKIKKDDVEVVEYLKATNPELFKTLKKEARAKRKERVPH"
     gene            complement(39742..40593)
                     /locus_tag="PLH85_02665"
     CDS             complement(39742..40593)
                     /locus_tag="PLH85_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB63487.1"
                     /translation="MTASSQMLTVADHNRSFSALTYVYPVVSRRARGVSVGINLNPNN
                     ACNWHCAYCQVPGLVRGAAPSIDIELLAAELQGFLTELQQGQFMKDHVPEEMRVIRDI
                     AFSGNGEPTSCKAFADIMERVVGIRNACGLRNVPIRVITNGSLADRPSVQAAFRTLAE
                     AGGEVWFKVDAGTREEILKINGVDIDPAAHARRLALTASICPTWVQTCVFRWDGELPT
                     QEFSDSYVAFLQKAGLEGLKGILLYGVVRQSHQPEAIHLQPLSAEELEHIAVALRKTG
                     LTVNVSP"
     gene            40659..41102
                     /gene="queD"
                     /locus_tag="PLH85_02670"
     CDS             40659..41102
                     /gene="queD"
                     /locus_tag="PLH85_02670"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNB63488.1"
                     /translation="MRITRRLEFDAGHRIPDHASQCRHLHGHRYALEITLSGDVVRAD
                     GAPINGMVMDFADVKAIAKEHVVDVWDHAFLAYRHDTAVVGFLQSMPGHKTVILDVVP
                     TAENLAAEAFRILDPLYRDVYGNHLRLERIRLYETPNCWADALRD"
     gene            complement(41119..41814)
                     /locus_tag="PLH85_02675"
     CDS             complement(41119..41814)
                     /locus_tag="PLH85_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB63489.1"
                     /translation="MAYVIEPPRPVVVPVLGDGLFPVRRVYCVGRNYAAHAREMGADP
                     TREAPFFFCKPADAVVSVGPEGVLELPYPVATANLHYEAELVVAIGLGGADIPVDEAL
                     AHVWGYGVGLDMTRRDLQTALKDKGRPWELGKAFDRSAPLSTLVPAGAIGHPQRAAIW
                     LERNGESRQRADIADMIWSVAEIVAHLSRYFRLEPGDLVFTGTPDGVGPVVAGDVVEA
                     GVEGVGRLRVRYV"
     gene            41965..42543
                     /locus_tag="PLH85_02680"
     CDS             41965..42543
                     /locus_tag="PLH85_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNB63490.1"
                     /translation="MTKKILMICGDFCEDYETMVPFQALLAVGHRVDAACPGKKAGDT
                     IATAIHDFEGDQTYTEKRGHAFALNATFAELRPEDYDALLIPGGRGPEYLRTYPTVIA
                     AVRHFFEAGKPVAAICHGAQLLAGARVLEGRTCSAYPACRAEVELAGGTYADIPIDQA
                     VTDGNLVTAPAWPAHPAWIAQFLAVLGTRISL"
     gene            42615..42971
                     /locus_tag="PLH85_02685"
     CDS             42615..42971
                     /locus_tag="PLH85_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNB63491.1"
                     /translation="MCQIFINADPDLYATRSRSLRLHGVATSLRLENLFWQVLEEIGA
                     RDGLTVPQLTSRLYDELLQSGRLDDSTNFASFLRVCCLRYLRLQLSGRIPADTAVPIR
                     ELDPAWVLTAERHPCA"
     gene            42983..43687
                     /locus_tag="PLH85_02690"
     CDS             42983..43687
                     /locus_tag="PLH85_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HNB63492.1"
                     /translation="MIDLHYWTTPNGHKITLFLEETGLPYRIVPVNIGRGEQFQPDFL
                     RIAPNNRIPAIVDHAPADGGAPVSVFESGAILLYLAEKTGRFLSTELRVRLDTLQWLF
                     WQMGGLGPMAGQNHHFVQYAPERIPYAIERYVKETARLYAVLDRRLADRDFIAGADYS
                     IADMACYPWIVPHDRQQQDLAHFPHLARWFDAIRARPATVRAYALAERIHAAPVVDDA
                     ARQVLFGQDAGTVKRP"
     gene            43799..45556
                     /locus_tag="PLH85_02695"
     CDS             43799..45556
                     /locus_tag="PLH85_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB63493.1"
                     /translation="MTDAIERFDQLDLQPAILAALAEIGYETPSPIQAACIPHLLAGQ
                     DILGEAQTGTGKTAAFALPALDRVDLARREPQVLVLTPTRELAIQVAEAFQRYASRMA
                     GFHVLPLYGGQSMVVQLRQLQRGAHVIVGTPGRIMDHIERKSLNLDGLATLILDEADE
                     MLRMGFIDDVEWILEHTPAERQTALFSATMPDAIRRVAHRYLREPREVKIKAATATVS
                     TIHQRYLQIAGAHKLDALTRILEVEDDFDAAIVFVRTKTATVELAEKLEARGYAAACL
                     NGDMTQQLRERVIEQLKAETLDIVVATDVAARGIDVPRISHVINYDIPYDTEAYVHRI
                     GRTGRAGRTGNAILFVAPRENHLLRQIERATRQPIEALKLPSREAVADKRVAAFRQQV
                     AEVLASEELDFFLDVIAGIEDAHDAEIHEIAAALAFLAQKERPLQMPDNGQPDIAQFA
                     GSTQRERPARENRGEILERRRDYAEGRLMRYRIDVGREHLASPRDIVGAIANEGGIDS
                     RYIGQINLYEDYSTVELPANLPPDVVQTLRRARVRQQALNLRPASAEESARPRGGRPA
                     GKPPRKDGPGGFKPRKPRA"
     gene            45763..46389
                     /locus_tag="PLH85_02700"
     CDS             45763..46389
                     /locus_tag="PLH85_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNB63494.1"
                     /translation="MFGRLLPQEGQFFEYFNAHAAQIVLGAQELVALMEDLSSADRRI
                     SAIEGIEQSADRITHDTIAQLHKSFITPLDRDEIHSLINAMDDILDTVQDVAECTTLY
                     HLQRSTPEAVQLANISLSCCTRVKIIVGLISNMDNASAILQTCQEIDRLETDADRIMR
                     AAMSRLFRDERDALQVMKLKAIYEQLESITDRCEDVAKIVEGIVLENA"
     gene            46397..47404
                     /locus_tag="PLH85_02705"
     CDS             46397..47404
                     /locus_tag="PLH85_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNB63495.1"
                     /translation="MESLHISLWVVTGLVVVALLFDFMNGFHDAANAIATVVSTGVLK
                     PHQAVAWAAFFNFVALFVFHLKVASTVGKGIVDPAAVDHFLIFGALIGAIAWNVITWY
                     YGIPSSSSHALIGGLVGAAMAKAGTAPLISAGIWKTVAFILVSPLVGYLLGSLMLVIV
                     AWICRHKPQRRVDKWFRRLQLVSAALYSLGHGGNDAQKTVGIIWMLLIAAGVTAQGDP
                     VPMWVVVLCYLAIGLGTLFGGWRIVKTMGQRLTKLKPVGGFCAETGGALTLFMASSLG
                     IPVSTTHTITGAIVGVGSVRRTAAVRWGLAGTIVWAWVLTIPCSALIAALAYWAGLQI
                     M"
     gene            47473..47715
                     /locus_tag="PLH85_02710"
     CDS             47473..47715
                     /locus_tag="PLH85_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63496.1"
                     /translation="MAPPTLKQQKTFALIRIIGGMASAGVLGYSFVSNVLAGAPAEGA
                     LLATGLMAFLALAYAGYYTRVLGRVAQAEQQAADKP"
     gene            47712..48965
                     /locus_tag="PLH85_02715"
     CDS             47712..48965
                     /locus_tag="PLH85_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HNB63497.1"
                     /translation="MKQRRLLAAWTLAATLAGCATTGPLESRPSPTLPPAPGSPTPRA
                     PADIDRPRPVSPAPAPIGTAPTPPAPPPIPSAQDGRALIARFLPPGVSDREGWATDLH
                     TAFTALHLSLTPDTICAAVAVIEQESSFQADPAVPGLPKIVWRELEARRQRYHLPRLM
                     LDAALLAPSPDGRSYRARIDALRTEKQMSALFEEMISEFPQGKTLFADYNPVRTGGPM
                     QVSIAFAQDQIREKPYPYPRRGSVRDEVFTRRGGVYFGVAILLDYPAPYSRLLYRFAD
                     FNAGRYSSRNAAFQNAVARLSGQALTPDGDLLRYRDGAPSSEPSATYSALSTLGGRLG
                     LSPAEIARDLRLEKTSQFAQTPLYIRIFTLADSLDGRRQPREILPEIDLKSPKIQRKL
                     TTAWFAERVDARHRTCLGRDRSFAP"
     gene            49104..50399
                     /locus_tag="PLH85_02720"
     CDS             49104..50399
                     /locus_tag="PLH85_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNB63498.1"
                     /translation="MTVQEQYAAKRMSAAEAIRHVHNGDTIIVPTAVAEPPALLNALS
                     EHRRDFHDVKVSQILAVRKMGYLDPTTAQHVRHVAYFFSGATRPGFHEDVVDFRPSYF
                     SEMPQLIERGLMPAEVVFTQASPMDAHGYFSLGLAADYTMAAISKARAVILEVNENVP
                     YAFGNNQVHISQVTALVENNEPVLEVGLPAIGPVQEAIGKYVADMIEDGSTLQIGYGG
                     IPDAVVMQLTHKHDLGIHTEMIGDGILTLIESGAVTNRRKNYLPGKMVATFALGSKKL
                     YNWIDRNPAFEMHPVDFTNDPYLAGLNDNLVSINATLEIDFLGQCGSESLGPIPYSGT
                     GGQSDFVRAANRSKGGKSFIVLPSTAKNDTISRIVPTLTPGTHVSTSKNDINYVVTEF
                     GVAQLRGKTAKERTRALISIAHPKFRDELTETAKKMHLL"
     gene            complement(50493..51338)
                     /gene="rlmJ"
                     /locus_tag="PLH85_02725"
     CDS             complement(50493..51338)
                     /gene="rlmJ"
                     /locus_tag="PLH85_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNB63499.1"
                     /translation="MLSYRHAFHAGNHADVLKHFVLVQLLEYFNRKDKPYLYIDTHAG
                     AGCYGLEEAFARKNAEFEDGVARLLARKDVPAELKSYLRIVRDLNPPGRLRLYPGSPC
                     CAAPLLREQDRMRLFELHPADNRLLHQTFAEAGSKVMIKEEDGFTGINAFLPPPSRRA
                     LVLIDPPYEVKTDYRTVLTALKECLRRFATGTYVVWYPRLTTMHARELPEKLKRLPLD
                     SWLHVSLDVKKPSEDGFGMHGSGLFVINPPWTLPETLKTVLPWLKDVLAQDDGADFVL
                     ESQIP"
     gene            complement(51421..51777)
                     /locus_tag="PLH85_02730"
     CDS             complement(51421..51777)
                     /locus_tag="PLH85_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNB63500.1"
                     /translation="MSDLDALQRRVKAFRDERDWGQFHALRHLIVSLNLEAGELLELI
                     QWRPDAEIDALPSDPVGREALADECADVLLYLLLIADRAGIDLGVAALAKLEKNAAKY
                     PVERFRGSRRKYNAPE"
     gene            complement(51785..52570)
                     /locus_tag="PLH85_02735"
     CDS             complement(51785..52570)
                     /locus_tag="PLH85_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNB63501.1"
                     /translation="MSDFVLFERRDSIVTLTLNAPETRNALSSPAEWEALVDACERIQ
                     ADLSVKAVILTGAGSAFCAGGNVKDFRDKRGLARGGGLDIRENYRSGIQRIPLAFHRL
                     DVPTIAAVNGPAIGAGCDLACMADIRIASESASFAESFVRLGIIPGDGGAWLLQRVVG
                     YSKAAEMSFTGDRLDAAQALEAGLVSRVVAPDVLLDAAMALAERIAAQPGHALRMTKR
                     LMREAQTLRLESLLELSAAYQALAHGSADHEAAVAAFLDRPKG"
     gene            52803..53324
                     /locus_tag="PLH85_02740"
     CDS             52803..53324
                     /locus_tag="PLH85_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNB63502.1"
                     /translation="MPIYRLGERQPELGEGVWIAENATVIGSVVLGRNVNVWFGSILR
                     GDNDPITIGENTNIQDGSVLHTDDGVPLTIGRNVTVGHKVMLHGCTIGEGSLIGINAV
                     VLNRAVIGKHCLIGANSLIPEGKVIPDRSLVCGSPGRIIRELTDHEISRLQASAEGYV
                     KNAERYVQELIKL"
     gene            complement(53460..54344)
                     /locus_tag="PLH85_02745"
     CDS             complement(53460..54344)
                     /locus_tag="PLH85_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB63503.1"
                     /translation="MHDRTEDPMKILSWNIQWGRGADGMVTLERTVSAVRTLGEFDVI
                     CLQEVAVAFPALQGGVPVDGVASIAAAFPDYSVHFASGVDMPDGNGGRSRFGNLTLSR
                     LPVGQVFRHSLPRPPEPGMPSMPRVCLETVVEGPQGWVRVMNTHLEYYSAVQRMTQIA
                     ALHTLQQDAVRLANAGGDTPGRNAAPGSPFEVLPRPASAVICGDFNCEPGSPEYFRMI
                     APIGPDVSGWEDAWRACYGDIPHLSTVGLNGAEWPDHAYCCDYFFVSADLVDRVAAVA
                     VDQLTAASDHQPIVLSLD"
     gene            54588..55439
                     /locus_tag="PLH85_02750"
     CDS             54588..55439
                     /locus_tag="PLH85_02750"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB63504.1"
                     /translation="MITPQELVACIEKLTSLPAVYHRIRALLDDPDSSVHDLAEAVSS
                     DPAITARVLRVVNSVLFGFPGRIETVGRAVNLLGMQQVHDLVLGTAIVGAFNGIRPAR
                     MHMSRFWKDSVYRGLAARAAAHRLGLGDPERMFVEGLLADIGHLVMFQAAPDMADAAL
                     ERAHRHHIPIHEAEQSVVGCHYAEVGAALASAWHLPAGFSAAIGAQLMPLLAGPHTQE
                     AAILHLANDLLASDADEVDEDAVLATISPSIVTILQADASMLGAVRARVRDELSAVIA
                     LFFPDQS"
     gene            complement(55449..57422)
                     /locus_tag="PLH85_02755"
     CDS             complement(55449..57422)
                     /locus_tag="PLH85_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNB63505.1"
                     /translation="MADKNRVGWYWKRLRDMSVSDIVGRAGSTAAQVVGRYRGAVPVP
                     PFRLDRTGARWLLPEVSAEGDFMGVADEVMSGHWRVLALDQAPLGLPPDWNCNPLDGE
                     HFPLNDGRTLSRREDGSGDIKYLWTLNRHRELVSLGLAWRKTGCRDYLDAIGVLMSDW
                     LEKSPYPHGPNWSSAVEVALRLMNWATVWQLIGGPNSALFAEPVGKVLRSDWLGSVWR
                     HAEFIQAHLSRPASAGSDRLGELAGLYVAGATWPYWSQAKVWRATGRDGLEQEVPRQI
                     SADGVVLEHSMAAQRMTWDVLSIAALAARATGREFSHALGCRLEAMAEYVAAVMDTGG
                     NVPQFGDSDDGDALGLGQAAGGCAYRSMLATGALLFSRPAFARKAGMLDPRTRWLMGV
                     DAARQFDLLLLKAFPSTLRTAFPEGGVYVLGAHLDTPLEWRIVADAGALGAGAMAEHG
                     HADALSFTLSVSGREFLVDPGSGTYHGQRAWRDAFRSTALHNTLVIGSEDQAVSGGRF
                     LWKRKYAVSVIRWESDADGDVLVAEHDGYRRLSGKPVHRRTWSFDRKTDSLSVRDEVI
                     ATMPQLVTLCWHFGEDCLVARSTKGVAVHNGPVRVDMVLPSGGDVSILQASEQPFAGW
                     ISRTYDRRVPATTVTWRGTLSPGDVLETEFRKG"
     gene            complement(57908..58726)
                     /gene="pyrF"
                     /locus_tag="PLH85_02760"
     CDS             complement(57908..58726)
                     /gene="pyrF"
                     /locus_tag="PLH85_02760"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNB63506.1"
                     /translation="MDFRSRLAAAWHSQDSLLCVGLDPDPARFPAHLKGRPDAVLRFC
                     LEIVDATADVACAFKPQIAYFAAARAEDQLEALIAHIHDRHPQVPVILDAKRGDIGAT
                     AQQYAREAFERFQADALTVNPYMGFDSVEPYLAYPDRGLIVLCRTSNPGGSDLQNLTL
                     SNGRLLYQHVAELVAEKWNLHGQCGLVVGATFPEELADVRQIVGDVPLLVPGIGAQGG
                     DVEATVTAGRTPLGAGLMISSSRAILYAGSGEDFAAAARQVALATRDDINRFRR"
     gene            59245..60465
                     /locus_tag="PLH85_02765"
     CDS             59245..60465
                     /locus_tag="PLH85_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNB63507.1"
                     /translation="MKKSILAQSVALACLAAAAGGAQAVSFTQNDVTVDINGTINGFY
                     VNRESKVTNLSTGVQTKTQNSELTNGLLPGWINFVVTSKAGDQDIKAHFSFAPGINNN
                     STVVGLPIGNNASGAGVTNPYSQIDTRNVYFQFGNNEWGSLKFGRDIGLFGQNIILSD
                     MTLLGVGGTSNAGIPYNTTFGMIGHGYMYTGFQAQITYSTPNYNGLQASAGIFQPSKF
                     AGDETKAPGFQAKVDYDWKGAMPGKVWGGLVSQNTSCSNASTTACAGMKPYNATGYEA
                     GAKAGYNNFEAVAYGFTGKGLGLSTIGAQFLGGSDGNGNKTDSKGYFLQGTYKMDKTK
                     FGINYGQNKDTGGGTQSVYGAPETKNKAYTLGVYHGLNKFVTLVGEFNNEKITNTATE
                     TKNNTISAGAILFF"
BASE COUNT        10400 a        21254 c        19389 g         9408 t
ORIGIN      
        1 ctcgtcctcg atcccacctg cggttcgggc accacggctt tcgtcgccga gaagtgggga
       61 cggcgctgga tcacctgtga cacgtcccgc gtggcggtga cgctggccaa gcagcgcctg
      121 atgaccgcca gctatgacta tttcgacctc aagtatcccc acgagggcct caagggcggc
      181 ttcatctaca agacagtgcc ccatgtgacc ctcaaatcca tcgccaacaa cccggagatc
      241 gacgagatcc acgcgcgcct gcacccggcg gtggcaacag ccctggcgcg gctcaacacg
      301 gccctcgccg acgggggcgt ggcggatttc gtcgtcaccg agggcggtcg caagggtcgc
      361 gcgctgcgcc tgacggccgc cgaggccctg ctggaatggg aagtgccctt cgagccgcct
      421 gccgactggc cggcggcggc ccgcgcggcc cacggggact tcctggaggc gcggcgcagc
      481 ctgcagaccc agatggaccg ctccatcgcc gcccacgcgg acccggaggt gctctacgat
      541 cagcccgcgg tggacaagaa ccgcctgcgc atcaccggcc ccttcaccgt ggaggcggtg
      601 cccttcccca gcgtgaaggg tttggacgag gcccaggggc ccgaggaggc ggatgcgagc
      661 atcgcccgca ccggcgagtc gggccgccag caccagtggc gcgacgaact gctcaagacg
      721 ggcatccgcg gcaagggcgg gcagcggctg aagttcgtgg acctggaagc cctgccctcg
      781 gaccgggaca tccgcagtct gcacgcgacg gggcacctgg attcgggcga acgggtggtg
      841 gtgagcttcg gcccggaata cgcggccctg gagcaacgcc aggtggccaa cgccctcaac
      901 gaggcgggcc agctcttccc cctgccccgg atgatcgtgt tctgcgcttt caccttcgac
      961 cccgaggcgg ccaaggacat cgacgggatg aagggcattg tggccctgaa ggcccagatg
     1021 aacacggacc tgctcaccga ggatctgaag aaggcccgtt cgggcaacca gagtttctgg
     1081 ttgatgggcc agccggaggt ggaggtgcgc cggctgaagg acggccgcta ccaggtggag
     1141 gtgcacggct tcgattattt cgacacggtg aagggggagc tggtctccgg cggcaagagc
     1201 cgcatcgccc tgtggtccct ggacacggat tacgatgacc gcagcctctt tccgcgtcag
     1261 gtgttctttc ccatggcggg caagggcgaa ggctgggaga agttgaagaa gaacatccgc
     1321 gccgagctgg acccggcccg cctcgacgcc ttccacggca ccgtgagtct gccctttgcg
     1381 gcgggcgatc acggcaagat tgcggtgaag atcgtggatg accggggtat cgagtccctg
     1441 aaggtgatga cgctggaaga caggaaggag ggccacccgc aatgagcacc cattcgcaag
     1501 gacgcctgat catggagaac gtcggcccca tcgtggcggc cgatgtgtcc ttcggtgatc
     1561 tgacggtgat cgtcggcccg caggcgacag gcaagagcat cttcctgcag accttgaagc
     1621 tgttgctgga ccgggaccat atccacgata cgttcttgca caacagcatc ggcttcagcg
     1681 gtgacagcgc ggcctttctg gggggctact tcggcaaggg catggccggg atgtggcggg
     1741 acggtgactc gacgctgaag tggaacggca agtcctcccc gctcaccgat ctggcccggc
     1801 cgagcaaatc gaggaaccgg caggagcgcc tgttctacat cccggcccaa cgggtgatga
     1861 gcctggtcaa cgggacgacg cagaatttcg gccagttcaa ttacggcgac ccttacgccc
     1921 tgcgctactt cagcgatgcg gtgcacggcc tggtccagaa cgagttcggg ccgcgcggca
     1981 agctcttccc gctccccaat cgcctgaacg ccgtattgcg caagcccatt gagcagcatt
     2041 tgttcggtgg cagccatctg gcgacggacg cgaaggactt taccaagcgc ctggtgctga
     2101 acgtggcggg ccatgacgag ggcctgccct atacggcctg gtccgccggc cagcgggagt
     2161 tcacgccgct gctgctgggg ctgtactggc tgtgcccggc gggtaaggtg acccggcggg
     2221 aacccatcga atgggtggtg atcgaggagc cggagatggg gctccatccc cacgcgatcc
     2281 tgaccatcct gctgctggtg caggagctgc tgcaacgggg ctacaaggtg gtgctgtcca
     2341 cccattcgcc ggtggtgctg gattgggtgt gggccctgca ggaattcaag cgccacgggg
     2401 ctggcgaagg ggatgtgcgc aggctgatga agctgcacgc caatcccttc tcgaaaaagc
     2461 tggcccaggc ggcgctggag aagacctaca aggtctattt cttcgaccgg atggcggagg
     2521 cgaaggatat ttcggacctg gacccgaacg cggaatccct ggacgaggcc caatgggggg
     2581 gattgatcgg cttcgcgagc catgtgggcg aactcatcgc cgatgtggtg aaccgggccc
     2641 ccgccaccga gcccacgccg caggacgcag cggctgacga tgaagcggag gacgcatgag
     2701 tcccgctcac acgcagccaa agaaggcggg gcagcatgca gcgcgccccg acacggcctt
     2761 cgtgcacgcg gtcaaggccg ccggcctgac cccggcagcg ggcaagggcg ccgttgaagg
     2821 caagtattcg gcgtccatcg tcgccacgaa ggagggggcg gtgtttacgg gcagtgtgga
     2881 cctggacacg caattcaagg cggccgagag ccgttccaac cgctgggact acggagtcgg
     2941 tatcaaggac cggggccagc cagaatgcgc catctggatc gagccgcaca gcggtacgag
     3001 cgcgggggaa gtcgcggtca tccttgccaa gctgaaatgg ctacaggcca agttggagag
     3061 cccgacgttc cacccactga aagtcctgac cgctgaagcg attcgtcagg gcaagacgcc
     3121 gtacgtgtgg cttgcgtccg gcaaggtcgg cattcgtgcc ggcagccggg aggcacgcca
     3181 gctggccagc gcggggatgc gtctcccgat gtcgcaggcg cgcatctgat gggcgcgcgg
     3241 gacacgatgg cccaaggccc tggatggctc acggcaggcc gctcgtcagg cgccctttgc
     3301 ccgcgctcgg ggcgggcggc ctttccgtgg cgcgtccacc gcttacgatg cagacgcccc
     3361 gccgacacgg gccgccgcgt ggagacctga acgatgcaga tcgtccgttt gaagctcaag
     3421 aactggcgca atttccggga ggtggacatc cccctcggcc cccgcacggt ggtgatcggt
     3481 gccaacgcgt cgggcaagtc gaacctgctg gacgtgttcc gctttctgcg gaccgtggcg
     3541 cagaaggacg gcggcggggt gcagaaggca ctgcgcgagc gggacgggct ggtcaagctg
     3601 cggagcctgc atgcgcgccg cgacccggaa gtgcggatcg aggtggaatt gcgcgcggcg
     3661 ccccccgagg aggcccacgt gtggcgctac gtgctggcgc tgagatcgga aggcaccggc
     3721 cagcagcggg tgctggtggc cgaagagcgg gtggaacggg acggcgtgcc gattctggag
     3781 cgccccgacg ccgccgaccg caaggacaag gagcgcctga cccagaccca cctggaacag
     3841 atcggcagca atggggattt ccgggcgctg gcggaaagct ttgcggccac cacctatctg
     3901 catctggtgc cccagctgct caagcacggc gatgagatcg cgacgcggat tccggagaac
     3961 gatccgttcg gccaggggct gctccagcgc attgcgaaga cccccaggca gacccgcgat
     4021 gcacgcctga agcgcatcca gaaggccctc gcggatgcgg tgccgctgtt ttcggagctg
     4081 cgtttcgagc aggacgcggt cacgggcctg tggcacctgg aagcgcgctt cacccactgg
     4141 cgcacccacg gtgcgtggca gcgggagaat cagttttcgg acggtacgct gcgtctgatc
     4201 ggcttgttgt gggctttgca ggagggggac ggcctgttgc tgctggagga gccggagctg
     4261 tcgctgaacg atgggatcgt ggcccgcgtg cccctgctga tcgaccgggt cttgcgcagt
     4321 cgcaagcgcg ccgcaacgtc gcgtcaggtc ctggtgagca cccacagcga aaccttgctg
     4381 gcgcattcgc aaggcgatgc gctgctgctg atcgagccga gcgccaatgg ttcgacaatc
     4441 cgccccccga ccgccgagga ggcggagcag atgcagcacg ggctcaatcc ggcggaggtc
     4501 ctgctgccca agacacgccc cgattccctg gaccagctgg gtctgtggga atgagccgga
     4561 ttctggtggt cggcgaagat cccttgtgcg cagccctggg agagcgcctg atcgcttcgg
     4621 cgctgccgtc ctggcaactg gccggcccct cgattccgtc ccgcagcttc agcgatctcg
     4681 tcccgaagtt gtcgcgttat gccggactgg cgcgggatct ccatccggtc ctgtgcattg
     4741 cggactcgga tcggaagtgc ccggtcgagc ttgtccgcga ccatcgcccg atgtgggcac
     4801 cggatcgctt tgtgtttcgg cttgcggtga gcgaagcgga gagctgggta ctggccgacc
     4861 gggagtgctt tgccgcggcc tttgaggtgc cgctcgcgcg ggtgccgagg aacgtggacg
     4921 gccttgccga cgtgaaggaa caattgcttt ccctggtgcg cgcgtcgcgc gtcagacgct
     4981 atcgggagga gatcgtctcc gaatcttcat gcccgcggcc gggccacggt tacaacctgc
     5041 atctgtgcgc cttcgtccgc gagcaatgga gcgcccagcg ggcccgacat gcctccccca
     5101 gcctcgaccg ggcggtaagc cgcctgcagg cgctgggcga ggcgccagcc taaaacatct
     5161 gccatgcatt cattgatcat cagttcccct tttgaacgtc ccacccatca ctggcgccgg
     5221 gtgaacgaca accgccttga actggtgccg gggcggcggt ctgccggcta cgaggtgttc
     5281 gatacgcgcc acaacacggt gcgcgcggtg gagctgagtc tggtcaatcg catccgggcg
     5341 cgcgtggacg cctggcgcgc ggcagactat cccggcgtga ccacggtgac ccggcagttg
     5401 ttggcccatt ggtgcgacag cgagaacagc ccacgccagc atgcgttcta tttttgccag
     5461 atcgaggcgg tggaaacgct gatctggcac gtggaagcgc cgcccgattt caaacagggc
     5521 attgcggtgc cgggtgacgg ggggccgtgg gagcgcctgt gcagcaagat ggcaacgggg
     5581 acgggcaaga cgacggtgat ggcgatgatc atcacctggc aggtgctgaa cgcgttgagt
     5641 tatccgaagc gcgggaagga cttttcgcgg gcggtctttg tggtggcgcc agggctgacg
     5701 gtgaaggagc gtctgcaggt gttgcggccc ggccatccgg acaactatta cgaccttttt
     5761 cacctgtgcc cgaacgaggg gatgcgccag aaactgaacc agatggcgct gggcatcgag
     5821 aactggcatc acctgatgcc gctgaaggag cctgaacgct ccgtggtgaa gaagggcgcg
     5881 gagagcgacg aggcttacgt gcggcgggtg ctgggccctg tggcggcaag ccgggacatc
     5941 gtgattctca acgatgaagc ccaccatgcc tatcgccagc gggcggaggt gaaggtgagc
     6001 cggaaggatg cggaggagct gggcatcgat ctggacgagg cgacccggtg gatcgagggc
     6061 ctggaccgca tccacaagat gcgccggatt gcccgctgct tcgatctgtc ggcaacgccc
     6121 ttcgcgccca ccggcaagac gagcacggag caggccttgt tcgagtgggt ggtgtcggat
     6181 ttcgggctca atgacgccat cgaggccggc ctggtcaaga ccccgcgtgt ggtggtgagg
     6241 gataacgcgc tgcccaatgc gcagacctac cggccgaagc tgtatcacct ttatcgcgag
     6301 cgggaggtgg ccgaggatct gaatcggcgt ggcgcgcaag cccatgaggc gctgccgcag
     6361 atcgtgcagg aagcctatac cttgctcggg gcggattggc gtgaaacctt gctggcctgg
     6421 caggccagcg gacacacctc gccgccggtg atgctgaccg tgtgcaaccg gaccgagact
     6481 gcggcgcgca tcgagcatta tttccgccac ggcgacgcct attggccgga gctgaaggcc
     6541 cccgagaaga ccttgcgggt ggattccaga gtgttggaaa aggcggagat gggggagtcg
     6601 tccgggagcg acaaggcata cgaagcggtg ctgcacgaga tactggaggc tgcagcgatt
     6661 cccgaagccc gcaaggcgga actccgggaa ctgaagaagg aggagttgct gcgagccgtg
     6721 gtggataacg tgggcaaacg caacacggcc ggacaggatc tgcagaacgt gatttcggtg
     6781 gcgatgctgt cggaaggctg ggatgcgaag aacgtcacac acatcatggg cctgcgcgca
     6841 ttcacgagcc agttgctgtg cgagcaggtg atcggccgcg gcctgcgccg ggtcacctat
     6901 gaaacggagc gggtacgctg tcccgacggg gtggagcggg atctgttcca gcccgaatac
     6961 gtgaatgtgt tcggggtgcc cctttcgatc ttccaggact ccgatggcgt gggcgcggcc
     7021 ccgccgccac cgaagcacag cacccaggtg gagtctctga aggatcgcgg cgaactggag
     7081 atcagctggc ccaacgtgct ccgggtggat caggtgctgc gggccgagtt gcatgcggac
     7141 tggtcattga tgccggcgct gaagctcgat cctgcgcaga tccacgtgag tgcggacctg
     7201 gcgccggccc tgggtgctgc ggccgatctg gccaaggcgg tgtccatcga tctggaaaag
     7261 ctgccggagg aattccggct gcaacgtctg ctctttgtcg cggcgcgcaa ggcgttcgag
     7321 acggttcggc atggctttac gggcagtcgg gaatccttga tccatcaatt gatcggtctg
     7381 gtggaagact tcttcgcgtc tgaccggctc gtcattccgt cggcgtttca ccaggacacc
     7441 ttgcgcaagc gaatcctgat cgcgctctcg atggacgcca ttgtccagca cgtgatcgag
     7501 cacgtgcacc agcaccatgc ggcacgcctg gagccggtgt tcgaccccga atttcccatc
     7561 ggctccactc gcttcatgcg gacctggtac acgacgaagc cctgcatcga aacaactcgc
     7621 tcgcaaatca gccatgccgt gtcggacagt acctgggagc attacacgac gcaggttctg
     7681 gagcggcggt ccgacgttgc ggcctacgcg aagaacgatc atctgggatt tcagctttac
     7741 tacctgtggc ggggaagccg gcggaaatac gttccggact acctgatccg cctgggcaac
     7801 ggcaagcatc tggtgttgga gatcaaaggc gaggattcgg accagaatcg ttgcaaacgg
     7861 ctcgcgctgg acgcgtgggt gcgcgcagtc aacacccggg gcgggttcgg cgtgtggtgc
     7921 tgggatgtgg tgaaaggtga accggcccgt gtggacgatg tcgtcggcca tcacgcccag
     7981 cgagggacga tgacgccccc catcggtgaa aatctcgacc ccgtccattg atcggcgggc
     8041 aggacctcac gcacgccact tgattggcca aagcggttcg tgctcgattg acacccgcga
     8101 ctttcggctt gaccgtgcag gctttgatgg cgcccgaagt cgtcggggtg atctgaagcg
     8161 ccgggacgaa cgccccgccc ctagcccttc agcaccctca ggaactcctc cgaatcgaag
     8221 ccgtccttca tgtgataggt gcgaccaccg cggcggatga ccaggtaggg ggtgccggtg
     8281 gcgccgtagg cgaggaattc ccgttcgcag cgccgatcca ggctcatgtt gcactcggtg
     8341 aaggcgaagc cgtactgggt gagccaggcg cgggcctggg cgcagtaggc gcattcggtg
     8401 gtggagtaca tcgtgacctc gccgggctgc acggtgctgg cgatgctgcg gagggcttcg
     8461 gcggtcgggc tgccgccggc gccgggatgg ggcatgtgct tccagccgta ctggagggcg
     8521 agcacgatca tggcgaagag cagcaggcgg atcggggtga gtcccatggg gcgtcctccg
     8581 gaaggcagcg ggcgtgcccg gtggggccgg gccgccggaa gactagcatc gtttcgctgc
     8641 cggctcgcgg atggacgcgc cgccaggctg aggccgtccg cccgccggca cgacgggcga
     8701 cgcgaacggg tggcggctca gccgcggcgg aagacggtct cggcggcctg accggggccg
     8761 aggtggccgc gccagatgac ggtggtgctg gcccggcgca ggtcgtagcc gcgggacacc
     8821 cagccgcaca gggggacgtc ggagccgcag atgacggtgg tctcgccacc gggcgggagc
     8881 gtcaccagca gggtgacgcc gtcgtgtccg acgacgaggc cgtcgggcgt gcaggtgatc
     8941 acgcagtttt cggcaaagtg ccagttcagg gccacggggc ggggcgcggg ggcgcccagc
     9001 tcgtcgcgca ccgagagggt gccgctgcgc ttgtcgaggc gccaggcacg gcgatgcacg
     9061 gggtggccgg cgaggcgccg gtagccgtcg tgctccgcca ccaggcggtc ggcgtcggcg
     9121 ctgctggacc attcgagcac gctgacctcg tatttgcgga tccacatgaa cttgccgccg
     9181 gtgatggcct ggtcctcgcc atccacggtg agggtgttgt gcagggcggt gctgcggaag
     9241 ccgtcgcgcc aggcgcgggg accgtggtag gtgccggtgc cgggatccac gaggaactcg
     9301 cgcccgtcca tcgacagggt gaaggacagg gcgtcggcgt ggccgtgggc ggcgatgccg
     9361 ccgaggccga gggggccggc gtcggcgacg acgcgcactt cgcgcggggt gtcgagggcg
     9421 ctgccgagga catacacgcc accgtccggg aaggcctggc gcggcgtgag gggttcgctc
     9481 cggtccacca gcagggtggc gaagtcgtcg gcggcggcgc gccccaggag ccagacgctg
     9541 cgcaggtcga agcggccgac cttgcgagca aaggcggagc gtttgaagag cacggcgccg
     9601 gtggcgagct gggagcggaa ggggtcgcgg tcggcgcaga gggacagccc gcaggcgagg
     9661 ccgtcgtcgg cgtcgccgaa ctggggcacc tgcccgcccg tgtccatgat ggcggccacg
     9721 tagtcgagca tggcctccat gcgttgccag taggcggtgg agaagtcatc cccggtggcc
     9781 cgcgcggcga ggccggcgaa gaggaggaag tcccacacga actgctggta ggacgtggcc
     9841 tgctcgagat tgacgccgtc cggcgcgttc tggcgcagga cctcggtctc caggccggcg
     9901 cgggcggctt cgcgccagcg gggtgcgtcc tcccagcagg gccaggtgac gctggccacg
     9961 tagaggcccg acagttcgcc gatcaggtgg ttgttggccg atgagtgcag ggagcggtgg
    10021 ccgcggatga actcggcgtg gcgccagatg ctgtcgagcc agtcgcgcag caggacgacg
    10081 ccttccggcg gggcgaacag gggcgactcg aagccgccga tgagctgcca cacgatggac
    10141 cagttgatga ggcggatgcc ggcttcgagg gcgctggacc agttgggacc gaggggatac
    10201 gggcactggc ggaaccagct ggtgaggagc gtgccgaggg cgtccaggta tttgcgcagg
    10261 ccggtggctt tccaggcctg ggcgaggacg accagctcca ggtggcggtt gggctcccac
    10321 aggtacttga tgtcgccgat gaccgtggtg tcgcggaagg ccatggtctt gccgaaagcc
    10381 atgggcagga cggcgccgtt gtggggattg cggttccatt ccggcggcag gccgagggcc
    10441 acggggcccc gcgcgaagat gcgccagcgc ccgccgagga tctcgtcggc ccggtcgatg
    10501 acggccggcg gcacgggggg cagctcgtgg gcgggcaacc agcaggcgcc gcgccgcgtc
    10561 aggtcgggcg gcgggatcgg cacgggccgg cgtcgccgtt cgagggcgtg gccggcggag
    10621 gtcgaggcgc gctcgaccat ttcggcgacg ctcatgcagc gcagccggtt ccagtaccag
    10681 cccacgcggg tgagtttgct catcggatat cctgcctgga gtggatccgg ctgacgatct
    10741 gccggaactt gccgttgcgc gtgcgttcga tggcgggcac ccgttcgatg cgcagcgtca
    10801 cctgcggacc gaggcgctcg cgggcgcgtt gcaggaggtg ttgttcgtcc tgggccgaga
    10861 attccggcgc gacgacgatg cggatgcgca cgaggtcgag gcgttcctgc acgacctggg
    10921 cctcgatgag gccgaagagc cccgcgaaga tccagtccag catcaccacg tggcgcccat
    10981 ccggcgtgcg gatgacgtcg tccacgcggc cgaggatgcg ctccacgacg cggaagctgc
    11041 gcccgcatcc gcactggaag tcggggtcgg ccagcaccac gcggtcatcc gagcggtagc
    11101 gcagcagggg catgctgcga ttgccgaagc cggtgccgat gagattggcg gtgccgtcgc
    11161 cgttgtcctc gaattcggtg acgccgtagt cctccatcac gtggtagcgg ccgtgttcgc
    11221 aggtgccgat ggcggccacc cgttcaaagg cgccgtacca gtcgaagacg gggcagccga
    11281 agcgctcggc gatgacgcgg cggtcgtcgg cgccgagggt ctcggacgag gtgacgatgc
    11341 cgcgcagggc cggcccgcgg taatggcggc cttcgtcttc cagcaggcgc gccagatacg
    11401 ccacggacga gggataggcc tgcagcagga ccgggtcgaa gctttcgagg gcgtgcagat
    11461 aggccagccc gttgtagcgg gacaggtgat aggaggacag catcagcatg ttgtccgccc
    11521 ggttgaagcg ccacaggggg ccgccctgct gccgcgtcgg cacgatggga tcgccgcgca
    11581 gccaggcacg ccggtcgccg ggccggtaac cggcccactc cgcctggcga tccacgaagg
    11641 cctgctcgaa ggtcaccgag gcctgatcgc gccagccgcg catggcgcgc ccggtggtgc
    11701 cgctggtgga gccctggaag cgcagccagg tgccgtcgcg ggcgagcagc ccatcaccga
    11761 tggagatcac ttcgtccttg gagagagccg gcacgcggct cagatcgtcc agggaggtcc
    11821 aggccgacgg gtcggtgccc aggcaggcga agacgtcctg gtaataacgg gtatggcggg
    11881 cggcatggag ggccagatcc cgcacggcgg cgaactgcag ggattgccag ccctcgcgcg
    11941 acagccactg gctgtcggcg atggcgtgac gggtctgggc gagggccgca ggccgggtca
    12001 gccagcgccg caggcgcgtc tgccaggaga gcagacgctc ctgcagggcg accggcgcca
    12061 gttgatacag cggggagcgg cacgagacga tcatcgcgcg aaacgctccg aagcgggggt
    12121 catcagcgaa tcccagaccg cattgaggag ggcgcggatg cgctccacgc cgtagtcggc
    12181 gagcacccgg cggcggcccc gctcgcccat ggcccgtgcc gcagccggat cggccaggac
    12241 gcgttcgagg gcggacgcca gggatgcgac gtcgtcggct tcgtgcaggg cgccgttgtc
    12301 gccgttgcgc accacctggg gaatgccgcc gacgcggctg gccaccaccg gcagaccggc
    12361 ggccagggct tccagcacgg acatgggcag gccctcgaag cgggacggca gcacgaagac
    12421 gcccgcccgc gcgaggaggc ggagcttctt gccgccatcc acccagcccg gcgtctcgat
    12481 gcggtcaagc acaccggccc gctcggcggc gtcgcgcacg gcgcggatgt caccgtcacc
    12541 ggcgatgacc gcgcgcaggt cggggaattt acgtgccaga cgggcaatgg ccttgacgag
    12601 cacgaagacg cccttggcgt cttccaggcg cccgaggaac aacagggtgg cgggctcccg
    12661 cgcgacgccg gccggcaagg tgacgggcag caccggattg ggcagcacgc gcacgtcggg
    12721 gtggccgacg ttgatgcgca gccagttggc ccagccttcg gacagggtga acaccacggc
    12781 cgccccgtgc agggtgcgat ccacccaggc ccgctcgagg aaggaggcgc ccgcgtagta
    12841 atggtcgaag gcgccgccgt ggaggtggaa gacgtagggc ttgccggcca ggcgggccag
    12901 ggcgaggaag acgctcttgc gccggaagct ggcgttggag gcggaatgga cgtgcaccgc
    12961 ggcgacgcgc ccggccgcca ggagcagcag gaagcgcagc agcgcagtga cgaaggcgcc
    13021 gagcttgcgc agggcgctgc catcccggta ggtggtgagc acgcggacgg gacgttcgtc
    13081 gagcagcccg gcctgcagcc agatgcgctg gacggaagcg atgccgccgc gggcctcggg
    13141 atcgggaccg agcatgatca gcgctggagc cttcttagac acggctgccc tcctccactt
    13201 cggatgtgac gtgctggatc atcaggcgcc gcttgccgcg cgccgtgcgg gcgatgtggg
    13261 gcaccacctc gaggctcggc tcgacgcagc cgaggagctt gacgcggaag tagttgtcga
    13321 gcaccgccac ggtgtcggcc ggcagcggcg agggcgactg caggcgcacg atgagcctgc
    13381 ccggctcgaa ctgttcgtac tggctggctt ccacctgcag gaactcgggc ggacgcaggc
    13441 cccccaggga ggccaccggg atgcgccggc cgtcgcggcc gacgaagaag tcgttgttgc
    13501 gcccgtcgat gcgctccagc acgggatagc cgggcaggtc cgggtgggcc gggccgtcgg
    13561 accaggcggc caggtcgccg gtgcggtagc ggatgaaggg catgacccgg ttgtcgaagc
    13621 cggtggccac cacctcgccc tgcacgcccg gttcggtgat gggccggccg gcttcgtcga
    13681 tgagttcgat gtggccgtac aggggccaca cgaagtagcg gtggtcgtcg ggcatggagc
    13741 cggccatcac ggagcgttcg gagtggccgt agtggatcag caccgggcag ccgaagacct
    13801 tctcgatgag ggcgatcatg tcgggctcgg cgcactcgga gaagagctgc acgccgcgga
    13861 tggcgtcgga gaagcgcggc accgggttga agtccagcca gcgggccagt tcatacaccg
    13921 cggaggaata gccctggatg aagctcacct tgtgctcgat ggcggcctgc acgtggcgcg
    13981 gcatgtagct gccgtccagg tggttggggc tgatccacag catgcgcttg atgggttcgt
    14041 agctccacag gggcccgccg tcggcctcgg agccgcgcac gttgcgcccg cgcagggaca
    14101 gcaccacgtc cttgcgggtg aggccgatgc gccggccgta ggactcgatg taggccgttt
    14161 ccttggcgcg ggagacgccc ttgtgcaggt agaagcggaa cggttcggcc accgagccgc
    14221 tggtctgcat gggcagccgc tcggccgggg agcagccggt ggagaggtag tgctccgggt
    14281 tgcgccggta gtccagcttg gtcatcagcg ggaacaggga cagccactct tctgccggca
    14341 ggtcggtgag gagctgcgcg gcgatggcgg cataggccgg cacggtctgg gcggcccaca
    14401 gcagggtttc gcgcaggcgc gcatcgcgca ggcggcgcag cgcctcggcg tcggtcagcc
    14461 ccgcctcgtc ccggtagcgg ccgaagtcct tgccgtggcg gatgctgtcc ggcagcagcc
    14521 catacacgcg ccccaggggt tccttgatcc attggggggc gtccatgtag cgcttcagcc
    14581 agcggtaacc ccagtcttcc agtcccatgt gctcgttccg tcggtcagcg tttcatccat
    14641 tcgcgggcaa acagcgccac gaacgccatg tcgtcgatga agtagcggcg gaacatgcgc
    14701 cgcggctcct gcaggaatcg gtagaaccac tccagcccgg ccttctgcat ccagaccggg
    14761 gcacgccgca cgaggcccac ggccacgtcg aaagtgccgc ccacgcccat ggcgaagggg
    14821 atcttcatgg cggcctgata gcgggccagg aagttctcct tcttgggcga gctgatggcc
    14881 acgaagagga tgtcgggccg cgtggcggcg atggcgttgg ccacaccggc ttcgtcctcg
    14941 ggcttccagt agccgttgcg gtagccggcg atggtcaggc cggggtagcg ggccgggtag
    15001 atctgcgcca cgcggctcac cacctcctcg cgggccccca gcaggaacac gcgccagccc
    15061 ttctcggccg accgctccat cagggattcg aagaggtcca cgccggtgac ccgttccttg
    15121 aggggcttgc cgagcaggcg cgaggcccac acgaccggca tcccgtcggc gttgatcagg
    15181 tcgcagccgt tgatgatgtt gcgcagcacg gggtcccggc tggccttgac gatcttgtcc
    15241 acgttcacca ccacgtgctg gtgcggtcgg ccggaatgga tgaagttctc caccgtcccg
    15301 agggtctcct ccatggtcag gttgtccacc caacacccca tcatttgtat tctcatcgtg
    15361 gcacctccgc gcggggcatg tcgcagcgtt cctctccgcc gccgtgcagc acggcacgac
    15421 gcagtgcggc gggcggcgga agcaggcggg aacgccgaat caggaaatcg aaggccgtca
    15481 gtccggcctc gtgactcagg gtatggacgc aggcgatgcc gggcagccag cccggatcga
    15541 tgcgccgttc ggtacgttct acgtactctt cggttgtcag tttctcgatg cggcgcagga
    15601 ccagggcgtg cccgtattcg ggcacgcaga cctgggcagg ccgcagcaga tggccgtccc
    15661 gctcgaacac cggaccggcc ggccgcgcca cgcgggcatc ggccaccacc gggttggcgg
    15721 cgtggggcgt ccacggcccg aggggggacg gggcatggaa ggcatgcagc tcgtcgtgct
    15781 tgtcggcgcc cggctcgctc acgttgccca gcagccacca gcggccgtcg tgggcaaaga
    15841 gggtggcgtc caccaggtcg atgtcggtga gcaggtcggt gaatttttcc cagcggtcgg
    15901 gcaggcgcgt gcagcggtac agctcgacac gccggttcgc gccgctctcg ggcaccatcc
    15961 acagggcgcc atcccaccag aacaggaagg ggtaggacag gtgccagggg cgcgccatca
    16021 ccggcaccgg cgcctcccag cggccgtccc gatggacgcg cagagcctgc agggtgccgc
    16081 gcccctcggc gtacagcacc tcttcgatga acacccacca gccgtcctgc ccggcgggcc
    16141 acaccatggg gtcggcccag aagcggtcgg gcggcggcag gatcagctgg gcgcgggcgg
    16201 gatcgaggca cacgtcgtcg ccgaaggcca gggcgatacc ccactggtcg cggctgagcc
    16261 ccttgcgcag ggcccgccgg gccgtacgcc cgagcaccgc ggcgacgcgg gcgaccgcgc
    16321 ccggcggctg cacggccgct gcgggcagcg tgtcgcgctg gggcggcggg ggcacctggg
    16381 tgcgcatccg gcgcagcgcg cggcgcacca tgccgatgcc cttggcgagg gcgcggcgcc
    16441 ggttgcggcg gatggaaaat tcgcaggtgg cccccacgga ggtatcgagg atcgcaccgc
    16501 cgcccacccg ctcgaggacc acgcgggtgg tggcgtcgcc tccgacgacg ggctgggcgc
    16561 ccgcccccgc gtccgtggca ggtcggccgg cgatgctcag catccagtgc tcggcggcca
    16621 gctgggggat cggggtgggc cgcggtcgca ggtccagcag cacgtcgcag gcccgctcgg
    16681 cgagccagcg cgccggcgcc acaccggcct ccgcacgcca cacgggcagc aggccgggca
    16741 gttcgaggga caccggatcg agggacgagg cgccccggtc gaagagcagg cgatccacct
    16801 gctccgccca gtcggccgca gccatgggct gcgcgtcata gaggacgccg acaaggatca
    16861 cgccgggctc cgcgccacac acgtcgagca ggcgcaccac ccagcccggc accgggccgt
    16921 ccggcagaaa gatggacaga cgcaggacgt tcatgcgaac acctccggtg cccggtgcag
    16981 caccgcccgg cgcaacagca ggttgcgcgc gaaggtcgcc cccagcagga ccgcgaagag
    17041 ccccagcacc gcgtcgacgc cctttggcac gaggagcatc acgcccccgc aggccaccac
    17101 gaagagcccc agcgaggcca ggttcaccac cagcggcagg cgggtctcct cgacggtctg
    17161 caggtagttg gcctcgggca gcacgagctg ccccacgtag aagctggcca gctgcagcag
    17221 gaagatggcg gtggcccccg ccagcgcgaa tgcgggacgt tcgcccacgc cggccatgag
    17281 cccccaggcc gccacggcga ccagggtgag gccgctgttc acacggaaca gggtgcgccg
    17341 cagggcatcg agcaaggccc gcacgtcggc tgcgccctgc tgccgcacca cacgggccag
    17401 ggccggcagg gcattgaagc tgagggcccg cgggaagagc agcagcacgc tggtcacgct
    17461 gccgatcagc gccaccaggc cgccgtaggc ttcgccggcc acctgcacgg tgcacggcac
    17521 gaggatcatg gacatgccgc cgctcacgaa gttgagggcg gcctgctcgg cggccgtgcg
    17581 ggcactgccg gccggcagcg cagccgcccc gccctggtcg cgtgcccgcc ggatcagcac
    17641 gaggaccgcg agggcgccga gcagcgccgt cggcacggca aaggcggccc aggccatgac
    17701 gggatgggcc cggccgaaga tcagcaggcc tgccgcgagg agcgcgacga ccagggtctc
    17761 cacgaccagc aggacccggt aggcgcccag ggcgaggccg tagtggcggg caaccgtgta
    17821 ggccgtccag ccggccagca gcagcgccgt ccagcccggt tcgaagaccc agccgcccca
    17881 cgccaggagg ggcagcggca ggcaggcgag cgcggtgaac acggccacgc cgcgggcgag
    17941 gcccagcatc accggcgccg agcgcccggc cggcgccacc ggcacccgcg ccagcaccag
    18001 ggccgaccac cccaccgcgg agaacaggcc cagcatcatg gcgatggcca ggtcattggc
    18061 gaagcggccg agggcttccg tgccgcaggc catctgcacc agcatgaaat gcagcaggcg
    18121 ctgtccgccg ggcgcagccg tcacggccag cacctcgacg cggcgcacga gggcggtgag
    18181 atcccggctc atgccgccct ccccgcctga cggcgctgcc acgcatcggc cgccgccacc
    18241 agggccagcg gcatgagcag gcccgcctgg ggccggaaca tgatgccgtt gctgaaggcc
    18301 gtggacgcca gcagggcggc cagcaagggc gccgccaccc cggccgggaa cagccgcgcc
    18361 cacacccaca gcagccacag cacgccgccc aggccgagca ggccctgctg attgagcaga
    18421 cggatcaggt cgttgtggga atccttggcg ctccaccacc agatctcgga gacgatccag
    18481 tcggacccct ccccgtgccc gaacaaccac tccagcaggg tgctgttggc caggatgtcg
    18541 gccttctgca gccacatgga catgcgcccg gaggacagtt ccaccatctc gtcccaggac
    18601 ggcaggtgca cgacgccgaa cagcagcggc aggtaggcca gggtcaggac ggcgacgatc
    18661 gtcaggaaga ccaccagcgc cgccttgcgg gaatgctgga agaaatagac gaggccgaaa
    18721 gccaccgccg ccaccatgga ggtgcgcaca ccccagccgt ggaacacgag gacgaaggag
    18781 aacaggatct gcgcgatgaa caggcgcttt ccgaagtggt cggcctcata cagatagagg
    18841 gacagcccgg cacacaccac gacgaggtag gagctggtgt gcagcgacgc gaaatacacg
    18901 ggccagaacg gctccccgcc acggttgagg gacggcggca ggaagagcag cgcgccgatc
    18961 acccccagcc acacgatggc cagggtgaac tgcatcaggc gcacgacgag ggcgggctcc
    19021 cgctcgaaga ggcgcagcag caccgccgac atgagaagcg gcgtgacgat cttgaggtat
    19081 tcgaaggtgg cgagcgtgaa gccgtcacgg gtgaggatct cggtcgtcat gaacgaggcc
    19141 agcaccagcg ggaccccggc cagcaccagg ggcgcgcggc gcagcagctc gtccgcgtaa
    19201 gtggccaggc cgacgaagat cgcgcacagc agcatggcgt cggccgcgcg gctgtagaac
    19261 gaatctgctt ccgcgtcgat caccggctgc accagcagca ccgacacggc cagcttggcc
    19321 cacagtccga cgaacatcgc ccacgtgagg gcccgcgcca cccggttttc caggtggaag
    19381 gccgtcagct cggcgacatc gaaggcaggc agggcgaagc gcatcagaac tccagcagga
    19441 cgccgccgag gatcgtcacg cccgcatcgc gcaggctcgc cgccagggcg cccgcccgct
    19501 tgtgggcggt gatgtgccgg cgcgccacca cgacggcaaa gccgcaggcg gcggccacca
    19561 gctgggcgtc ggcgccgacc tcgtcggccg gggtgtccac cagcaccatg tcgtagaagt
    19621 cgcgcgcggc cgtcacatgg gcggcgaagc cggagcgggc cagcaactcc tgaggattgg
    19681 gcggcacgtt gccggccggc tggatcgcca gccccggaaa gcgtggatga acgaccggtg
    19741 gctggctgtc gccccgttcc gccagatgcg ccgccagccc gcccgcgggc ggcagccgga
    19801 acagcgcctg ctgccgcggg gtgcgcaggt cggcgtccat caccagggtg cgttcgccca
    19861 gctgggagaa ggtgatggcc aggttcgcca cgagccagct gcgtccttcg ccgcggcccg
    19921 cgccgacgac ggccacggtc ttgcagtggg gattgccggc gcgacgcagg acgagctggc
    19981 tgcgcagctt gcgcagggcc tccacctgcg gcgcgtcggg cgtgaaggcg gccaccagct
    20041 ccggcgccac cgaaggatcg ccggccgcca gcacggggta atcgaactgg cgggcgaggg
    20101 ccgcgcgcac gtccgcctcg ctcaccaggc cgagctccac cgccgcctgg ccaaagggaa
    20161 tcccccgctc acgctggcgc gtggcgaccc ggctggcggc gtccgccgac aggcgcccgg
    20221 cgctcaccag caactcgccc agactgggcg tggcatcccg cgcgcgctcc agcggggcca
    20281 cgttgccgaa tccggaaaac ttcgctttat ccatgatctg tcctcaagcc cggcgcagac
    20341 gcggccagaa gggccgggct ccggcggctg cggccggcgc agccttcacc tcgaccacca
    20401 gcggcacgcc cagggcgtcc gccagatcgc cggcggcgcg cacccgccgg tcgaagagtt
    20461 cgcgcacgat ggtcaccgcc aggcccagca gggcgccgat gacgagggcc agacccacgt
    20521 tgaggaggac cttcgggctg ctcgcctcgg tgggcgccac cgccgacgac agcagcgcca
    20581 cgttgccctg acgggccttg ctctccaggc tggtctgggt cagccgcgcc atcaccaggt
    20641 cgtaggcctt ctgggcgctg tccacctcgc gctggagcag catcatttcg tcgcgctgct
    20701 gcttgagggc cagcacgcgc tgcttctggg cgtcgaggcg ccccttcagg tcgccttcgc
    20761 gggcctggct gatgcgggaa ttggtgccca ccatcgccgc cacgttgccg gtctcctgcc
    20821 gcaggcgggc acgcagggac tccacctcgc tcttcgtgcg ctggtactgg gggtggtttt
    20881 cgccgaggta gccggacagc tccgtgagct tggcctcctg gcgtgccaga tccaccttga
    20941 gggcctggat cagggggttg ctggccacct cgttggcccc gccgccctgg cgctggcgct
    21001 cggtggcctc ggtgctctgg gcctgaaccg cggtgaactg ggaggacagc tcgccgaggc
    21061 gggcgctttc caggtcgagc ttctcgtcgc ccaccaggcc gtgctcccgc tggaaggcgg
    21121 acaggcgcga ctgggcctgc tccaggttgg cgcgcagcac cttggtgtgc tcatcgaacc
    21181 aggtggcgta ttcgcgggcc ggctcgatct tcagttccag gtcggtttcc aggtaggcct
    21241 tggcgagcgc gttggcgtag ttcgcggcaa acgcgggatc gtccgcggtc acggtgatgg
    21301 tgagcacgtt gctttcgtgg gacgggctga ccttgacggt cttcttgagc ttgccggcga
    21361 tccagcgcgc cggatcgccc tggccgccgg aggccttctg ccagcgctcg gcccaccggg
    21421 gctgatccat cagcttgagg gccgccaccg cgcgggcccc gacccgttcg ctggcgatga
    21481 tgtcggcctg ggtgacgagg aagcccggcg tggtgaggcc cacgccctgg ggcggtgcga
    21541 gggggtcgga gccgcgtgat tccaccacca cctcggcggt ggcggcgtac tggcgcggca
    21601 ggatcagact gaccagcagg gccaggagca cgacgccgcc ccacacgccg aagagcagcg
    21661 tgcgacgcgc cttgagtact gccaggagtt gggtgattcc catgatcggg gttccgtcag
    21721 aacagggctt cgcgcacgac gatcacgtcg tccgcctgca ccggatcgtt gagttggggg
    21781 ttgatgacgc gcacgctgcc gtcgtcgccg cggcggtgga tgcggatgcc ccgctcggtg
    21841 ccgcgggggt tgaggccgcc gcccagggac agggcctgca gcaccgtcat gttgcgttcg
    21901 acgcggtagg cgccggcctt gaggacctcg ccatacacgt agaactgggg cgcccgctgc
    21961 acgtagagcg tgtcgccggc ctgcaccggc acgtccttct cgggcgtcca ggtgacgaag
    22021 aggtcggcca cgtccacctc cagacgcacc cggcggccgt tgcgcaggcc ggtgaggatc
    22081 accttctcgg cgccctgggg cgtggtgccg ccggccaggg ccagggcgtc ggtgaccttc
    22141 atcgcggtgc tttccagcgg atagcggccg gggcgcgcca cctgcccgag caccgccacc
    22201 tgggcgccgc gcacctgctg caccaccagg gtcacctggg gctggagcac atagccgccg
    22261 ctgcgcagac ccgcggcgat ggccttctcc gcctcgccga cggtgagccc gcccacctgc
    22321 acgctgccca ccagcggata gctgatgacg ccggtctcgg acacccgggt ctcgagcccc
    22381 agatcggggt tctggaagac cgaaatcttg accccgtcac cgggaccgag tcgatactcc
    22441 tgcgcggccg gagccgggcc ctgcgccagc gcgaggctgc ccgtcagcgc ggcggccagc
    22501 attgcgacca cgcacagcag ccagcgttgc cagccctccc taccctgctt caaaataccc
    22561 actgcagact ccctgccaca atccaatccg tgaatgacca ttcctgctgg ttcgagtccc
    22621 gccgttcccg gctcaggctc gcggtccagc gggtccgggc cgacatcgcg tggagcgccg
    22681 tcacccccac cagcgtcgcc gtgtcctcgc ggcggcttgc ggccaccacg ccgaatccgg
    22741 gatcgccgcg gtactgggcg ttgcgccact cgtagcgact ctcgatgcgt gtcttcggcg
    22801 agtactccca caccgcgccc aggcgcgcac ccttggtggc cacgtaactc gacacaaaat
    22861 cctgggccgg ctccacgtca tagcggagcg agccgttcag ctgggtgcgc ggcgtgacct
    22921 gccattcggc gccgatcagg ccgttgcgtc ccgaaaaatt gctctgggga tagcgttcgt
    22981 aattccggct gacacgcccc acgcgcatca ggtagcgcga gccgctgcgc accggcagcg
    23041 acacttcgag gaaggtctcg tcctgattga agcgcgtctc cgcaagggag cccgccgtcg
    23101 ccggcaggtt ggggtattcg ccccgtgccc ggcgccgcgt gagggccacc gcgccgccgc
    23161 gctccggtac gtagcgcagc acaccgtcca cgccggcaaa atcgatctcg ttggtgggac
    23221 gcgtgccatc cttgtagcgg cggctgccgc gcccggcctg cgcgccgatc tgccagccgc
    23281 ccaccagggt gtaaaacgca ttgccgccca tctggcgcgt cgtcgagatg ctgcgcaacg
    23341 ggctgcggta gtaggcgtag tcgtccagcg cctcccggta ttcggatacc agctcgccct
    23401 tcagatcctg cccggccttc cagcgcaggc ggccgatgcc accctggacg gtgtgatcga
    23461 ggaagccgaa gtggctgtag cgggccatgg tcgcgtcgta gtcgaggagc agctgctggc
    23521 cggcgtagga cttgtccagg cccagaccca gcttggtctg actctgccag gagcccaggt
    23581 gggccagtcc ggcggccacc gccgccgcgg aatcccgcag gcggaaaacg ttgctgtcgt
    23641 aggtcaggtt ctcggcgacg gaaatccgcg tcccggcgct cttgccgacc ggcagatcca
    23701 gcggctcacc tccccgctcc ggcaatccgg ccgcttccgg ccccagggtc ccggtgagcg
    23761 tcagggcggg aggatcgagc tcgtcgctct cgaacagggg ggcggcctga cggctggctt
    23821 cgccgagcag ggtcgacacc cgcaatgcgg acgcgtcgag ggcgagcacg gggcttccca
    23881 cgcccagcat gagcactgct ccccccagtt gtaagccgat gcgccgcaaa acaagttgcc
    23941 cctcaagttc gaaacacgcg catcttgaat gagggaatcg cgaaaacaag cgaactaata
    24001 cgcgatttgc aaaatctttg aactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24121 nnnnccgcat gggcgcgtca cgccgcgaag gcaagcagct gctcggattt ttcgacgatc
    24181 gtcatgccga gcgtctggcc agtccgcgcc tgcggctgct tggacgcatc gccgatctgg
    24241 cggattacgt gcgccagcag ggtgtggacg tgatctacat cgccctgccc atgaccaccc
    24301 agccacgcat cgtccgtctg ctggacgatc tgcgcgacac gaccgtctcg gtctatttcg
    24361 tccccgacct gttcgtcacg gacctcatcc aggcgcgggt ggatcatgtg ggcggcatgc
    24421 ccgtggtggc ggtctgcgaa acccccttca cgggcctcaa cggcgtgctg aaacagtctt
    24481 ttgattttct gatggcggcc tgcatcctga tcctgatcgc cccgctgatg ctggtgatcg
    24541 cggcggccgt gaagtgctcg tcgccggggc cggcgctgtt ccggcaatac cgctacgggc
    24601 tcgacgggga acgcatcctg gtctacaagt tccgctccat gacggtctgc gagaatggcg
    24661 agaccatcgc ccaggcccgc cagaacgatg cgcgtgtgac gcccctcggc gccttcctgc
    24721 ggaaaacctc tctggacgag ctgccccagt tcatcaacgt gctgcagggg cgcatgagca
    24781 tcgtgggccc gcggccccat gccgtcgccc acaacgagat gtaccgcaag ctcatcaagg
    24841 gctacatgat ccgccacaag gtcaagccgg gcatcacggg ctgggcccag gtcaatggct
    24901 gccgcggaga gaccgaaacc ctcgagaaga tgaagcagcg tgtggatttc gacctggagt
    24961 acctgcgccg ctggtccctc gccctggacc tgcggatcat cctgcgcacc gtccggctga
    25021 tccgcaagga tcccaacgcc tactgaaggg agggcgcatc cggccggtct ccgtcaccgg
    25081 aacacggccg atcgagggct gacactgcag cgcagcggcg cgaaatgaaa aaagggattg
    25141 cacgaatgca atcccttttt gtctggcgcg cccggcagga ttcgaaccca cgaccccttg
    25201 gttcgtagcc aagtactcta tccaactgag ctacgggcgc ttgcaactcc caactccagg
    25261 acgctgacag aaccgccccc tgaagctgca agagccgctt gtcgcgactc ttgaagtaaa
    25321 ttggcgcgcc cggcaggatt cgaacccacg accccttggt tcgtagccaa gtactctatc
    25381 caactgagct acgggcgcgc tgaagaaccg gcattataca cacattgcgc gaaaatcaaa
    25441 gcctcccggc ggcacgtccc gacgcggcac cctgatgcac gtccccgtga ggccgatacg
    25501 gcgttcccct gagccccgtt ccggtgcaca ctgtccccaa aaaccacgag aacagcgcac
    25561 ccgccatgca caccctcgcc ctgcgcggcc acaccctcga ggtcgtccgc cgcccttccg
    25621 cccatccgcg cccggaggcc ccagcccttg tcttcctgca cgaagggctc ggctccatcg
    25681 ccatgtggcg cgacttcccc caacgcgtgg ccgacgccac cggctgcgag gccatcgtct
    25741 attcgcgtca cggctacggc cgctcctcgc cgctgactgc accgcgcaca ccggactaca
    25801 tgcatgtgga ggcgctccag attctgccgg ccgtcctcga cgccctgcag cttcgcgcac
    25861 cgatcctcgt cggtcacagt gacggcggct ccatcgccct catccacgcg ggcggcaccc
    25921 cggcgcccgt ccgtggcgtg gtggtgatgg ccccccacct ttacgtggag gatgtggcac
    25981 tggagggcat ccgccggacc gtcgacgcct atcggaccac ggacctgcgc gcgcgcctcg
    26041 cccgctttca tgccgacgtg gatggcgtgt tcgcgggctg gagcgacatc tggctgcacc
    26101 cggatttccg cgactggaac attgaagcct tcgtgtccgc cattccctgc cccgtgatgg
    26161 ctatccaggg cgaggacgac gagtacgcca cgatggatca gatcgaccgg atcgcccgcc
    26221 tcgcgccgga tgtggatctg gtgaagctgg cggactgccg ccactctccc cacaaggatc
    26281 aggcggatgc cgtcgtcgag gccatcgccg gcttcgtggc ccgcctgctc gacgatcccc
    26341 actgatttcc cgccaccacg gagccatgcc acagatgcgc gcctatctct gccaggcttt
    26401 cggccccctc gaccagcacg gaccgggaca gatcggcgac ccgcagcccg ccgataccga
    26461 gatcgtcatc gacgtcgccg ctgccggtgt caattactac gacaccctga tcgtccaggg
    26521 tcgctaccag atccgcccgc ccctgccgtt ctcacccggc ggcgagtttg ccggcaccgt
    26581 gtgtgccgtg ggcgcgcagg tccatgaatt caccgttggg caacgggtgc tcgccttcaa
    26641 ctccttcggt ggctacgccg agaaggcccg ggcccgggcc gcgcaggtgt ttccgttgcc
    26701 cgacgcagtc agtttcgaaa gcgctgcggc agccctcgtc acctacgcca cggcctggtt
    26761 cgccctgcac gatcggggcc gcatgcagcc gggcgagacg gtgctcgtgc tcggcgcggc
    26821 cggcggcgtc ggactggcag ccatccagat cgcccgcgcc gccgggtgta ccgtcatcgc
    26881 cgccgccagc agtcgggaaa agctggacct gtgtgccgcc gccggtgcgc acacgggcat
    26941 cgactaccat gcaaatgatc tcaaggacgc cgtcaaggcc gccaccggcg gacggggcgc
    27001 cgacatcgtc gtggatgtgg tgggcgaccg ttataccgaa gccgccgtac gtgccaccgc
    27061 ctggcgtggc cgcgtcctga tcatcggctt tgcggcaggt gagatcccgc gggtgccggc
    27121 caacctgctg ctcctcaagg gatgcgagat ggcgggcgtc ttctgggacg agttcctgcg
    27181 ccgggaaccg gccgaatgcc ggcgacaggt ggcagcgatc ctcgacggca tcgcccgggg
    27241 catcttccag ccccccattc acggacgtta tggcattgag gaggcaggcc aggccctcac
    27301 gctgctggct gaccgccagg ccaccggcaa gctgctgatc gtgccctgaa atccggaccc
    27361 ggcccggatc ggtgatggac cgctaccggc cgcgacaccc ggcgccggtc aaggcaccgc
    27421 cgccagcccg cgggaccacg cctatccggc catcgcgagc ggcccgtcgt cgccctggag
    27481 acccgccttg cggttcatcg gcagggtgcc gcgacactgg ggaaaacgcg agcaccccca
    27541 gaacggacgg cggatcacgt tgcggacgac catccgctcc ccgcattccg gacacgccgg
    27601 agccgtccag tcccccgtcg ccgcgaggtc cagcagacgc tgctggctgt cttcgggaag
    27661 ccgccggatc aatgccagca tcaatctgct gtcgaacagg gtcatccgcc cctgcgcggc
    27721 aaaggccagc gcgtccgggg cgaagccgcc gggggccatg aagaacgccc ggtcggcgct
    27781 ttcccgtcgc cgggtttcgg ccagcgcccg gaccgcccac acgtcgatgg tcggtcccca
    27841 cggcacacac cgcacgacgg cggtcaccgc accggggcgc cgtggatcct ggtgcagacg
    27901 gatgtccccc gcgctgctgt cctcatcccc cgcgtgggtc gtggcggcca ccccgctttc
    27961 ccggaagaac gcgcagcaca gcgcctggaa gcgcgcacga tccagctgtt gcagcacgtc
    28021 caccgaccac gcgcgtgcgc gccggggctg acttcccgat ggcgccgtcg ccaccgcagc
    28081 ggcgccttcc gtctgggcca cccgatgcgc acgacgtccc tgtgcccatc gtcccgccag
    28141 acctgccgcc aggacgagtg cgccccaccc cacccctctt gcggtttccg tcagggcctc
    28201 tccgctcagc gatgtctgcg acacgattac tggaaccgcc agcacgccca gcgcgccgcc
    28261 cgcaaccacg ccgaagatcc gcccccagcg tcgcatggcc gaggaatgga cttcgtcccg
    28321 agctgaatcc gctgcgccaa gcatgttccc gacatccttc tatgcaaagg ccgaatgtta
    28381 ccccttcaac atagcccgga caacccgcat gtcatcttcc aggcccgctg cggacacgtg
    28441 gggttgccgt cggatcggcg tcatgttcag ggagattcac ggcgcgcccc agtgcgtcta
    28501 agatcacgcg cagaatttca ccgtccacag cccatgaatg acgttctcca cgccgccgtc
    28561 accgacgccc tggcacgcca cgggcaccgg cccgacgccc tgcttcccct gtttcacgcc
    28621 ctccaggacc ggatcggctg ggtgcccccc gacgccgtgc cgatgttggc caccgccctc
    28681 aacctgagcc gcgccgaggt ccatggcgtg ctgagcttct atccccactt ccgccgtacc
    28741 cggcccggcc gccacgtgct gcagatctgt cgtgccgaat cctgtcaggc cagggggggt
    28801 gaagccctgg aggcgcatgc ccgcagtctg ctcggcaccg gctggcatgg caccagcgcc
    28861 gacggcgcgg tgacgctgga gccggtcttc tgcctgggca actgcgcctg ctcccctgcc
    28921 gtccgcatcg gtgacgaact cctcggtcgc atggacgtcg cccgcctgga ggcgctgatc
    28981 gcccgcctgc gcacatcgga gcccgcccca tgaacgtccg cctcttcgtg cccgccgact
    29041 ccggcgccct ctccgtcggc gccgacgacg tcgcccgtgc cctggtccgc gaagccgcct
    29101 cccgcggcgt gacggtcgag ctcgtccgca acggatcccg tggcctgtac tggctggaac
    29161 ctctggtgga ggtggacacg cctgccgggc gccttgccta cggcccggtg agcgtggagg
    29221 acgtgcccgg cctcttcgac gccggtttcc tgaccggggg catgcaccga ctgggtcatg
    29281 gcctcaccga gcacatccct ttcctcgccg gccaggaacg cctcaccttc cgccgtgtcg
    29341 gcgtgatcga tccccgatcc cttgcggact accagaacca cggcggcttc gacggtctgc
    29401 gtcgggccct tgccctgggt gatgccgccg tcgtccgaga agtcaccgac tccggcctgc
    29461 gcggccgggg tggcgcggcc tttcccacgg gcatcaagtg ggccaccgtg cagacggccg
    29521 gcgacggcga caaatacgtg gtgtgcaatg ccgacgaagg cgactccggc accttctccg
    29581 accgcatgct gatggaaggc gaccccttct gcctcgtcga gggcatgacc atcgctggcc
    29641 tcgcggtcgg cgcccgccgc ggctacatct acctgcgggc cgaatatccc catgccttcc
    29701 gcgtgctcga agccgccctg gccaccgcgc gcagcgccgg ctggctcggc gacgatgtgg
    29761 ccggtagcgg gcaagccttc gacatcgagc tccgactggg ggccggggcc tacatctgtg
    29821 gcgaggagac ggccctcctg gaaagcctcg aaggccgccg cggcatcgtg cgagccaagc
    29881 cgcccttgcc ggcgctgagc ggcctgttcg ggcgccccac cgtcatcaac aacgtgatga
    29941 gcttcgccgc ggtgcccctc atcctcgccc gcggcgccgc cttctaccgg gattacggca
    30001 tgggccgctc ccacggcacc ctgcccttcc agctcgccgg caacctgcgt catccggggc
    30061 tcgtggaaaa ggccttcggc atcaccctgc gcgaactcct cctcgactac ggcggcggct
    30121 ccgccagcgg acggccgatt cgcgccgtgc aggtgggcgg cccgctgggc gcctacattc
    30181 cagagtcccg cttcgacgtg cccctcgact acgagaccta cgccgccatg ggcgcgatga
    30241 tcggccacgg cggcatcgtc gccttcgacg accgcgccga cctgggccgc ctggcccgct
    30301 acgcgctgga attctgcgcc ctcgaatcct gcggcaaatg cacgccctgc cgcatcggca
    30361 gcgtgcgcgg cgtggaactc atcgaccgcc tcctcgcagg taccgaccgg ccccgcgacc
    30421 gggccctgct ggacagcctc tgcgacacca tgatccacgg ctcccagtgc gccctggggg
    30481 gcatggcgcc gctccctgtc cagtccctcc tgacgcattt tcccgacgcc ttcggcctcg
    30541 cgccgaccgc tgcctgaggc acgcccgcca tgtccctgcc ctgccccccc gatgccggca
    30601 ccccggcccg cgacggcgaa gccaccgtcc gcctcctcat cgacggccaa ccagtctgcg
    30661 tgcccgccgg caccagcgtg atgcacgccg cccacacggc cggctgcgcg atccccaaac
    30721 tgtgcgccac cgaccgcctg gaagcctttg gcgcctgtcg cctgtgcctg gtcgagatcg
    30781 acgggcgcaa gggctatccg gcctcatgca ccacaccggt ggcgccggac atggtcgtgc
    30841 gcacccacac accgcgcctc gccgagctgc gccgcaatgt gatggaactg acgatctcgg
    30901 accatccgct ggactgcctc acctgcgccg ccaacggccg ctgcgaactc caggacatgg
    30961 ccggcgcggt gggcctgcgc gcggtgcgat acggcctgga cggtgccaac cacctgggcg
    31021 atcgggccga cgattcgaac ccttatttcc gctacgaccc gtccaagtgc atcgtgtgca
    31081 accgctgcgt gcgcgcctgc gaggagatcc agggcagctt tgccctgagc atcgtcggcc
    31141 gcggtttcga ttcacgggtc cgcgccggcc aggacgagtc cttcatggcc tccgagtgcg
    31201 tgtcctgcgg cgcctgtgtg agtgtctgcc ccaccgccac gctgatggaa aagtccgtca
    31261 tcgaacacgg ccagggcgaa cgccgcgtga ccaccacctg cgcctactgc ggcgtggggt
    31321 gttccttcga tgcggaggtc aagggcaact ccgtggtgcg catggagcca acccgcgacg
    31381 gcggtgccaa ccagggccat gcctgcgtca agggtcgctt cgcgtggggt tacgccaccc
    31441 acccggaccg cgtcaccacg ccgatgatcc gcccgagcat cgacgccccc tggcaaaagg
    31501 tgagctggga agaggccgtc acccacgccg ccgccgaatt ccggcgcatc caggccgagc
    31561 acggtcgcgg cgccatcggc ggcatcacct cgtcccgctg caccaacgag gagacctacc
    31621 tcgtccagaa gctggtgcgc acggccttcg gcaacaacaa cgtggacacc tgcgcccgcg
    31681 tctgtcattc ccccaccggc tacggcctca agcagacgct gggagagtcc gccggcaccc
    31741 aggacttcgc ctctgttctg aaggccgacg tggtggtggt gatcggcgcc aaccccaccg
    31801 acggccaccc ggtgttcggc gccctcctca aacggcgcct gcgccagggc gcccggctga
    31861 tcgttgtgga cccacgcgcc atcgacctgg tgcgctcgcc ccatgtggaa gccacccacc
    31921 acctgcaact gttgcccggc accaacgtgg cgatgatcaa cgccctcgcc cacaccgtgg
    31981 tcaccgaagg tctcgccgac gaagcctacg tgcaggcccg ttgcgacctg gcctcctggc
    32041 aggcctggcg cgacttcgtc gcccgccccg agcacgcccc ggaagccgtg gaggccgtca
    32101 ccggcgtgcc cgccgaagcc atccgcgcag ccgcccgcct gtatgccacc gccggcaacg
    32161 gcgccattta ctacggcctc ggtgtcaccg aacacagcca gggctccacg atggtcatgg
    32221 gcatcgccaa cctggccatg gccaccggca acgtgggccg ccccggcgtc ggcgtgaatc
    32281 ccctgcgtgg ccagaacaac gtgcagggca gctgcgacat gggctccttt ccccacgagc
    32341 tgcccggcta ccggcacgtt tccgacgcca ccgtgcgggg cagcttcgag gccgcctggg
    32401 gcgtgcgtct cgaccccgaa cccggcctgc gcatccccaa catgctcgaa gcggcgctcg
    32461 acggcagctt ccgcgggatc tacatccagg gcgaggacat cgtccagtcc gaccccaaca
    32521 cccagcacgt ggaggccgct ctggcccaga tggaatgcgt cgtggtgcag gacctcttcc
    32581 tcaacgagac cgccaagttc gcccacgtct tcctgcccgg cgccagcttc ctggaaaagg
    32641 acggcacctt caccaatgcc gagcggcgca tctcccgtgt acggcgggtg atgccgcccc
    32701 tctccggcaa ggccgactgg gaagccaccc tggccctcgc ggccgcgctg ggacatccga
    32761 tgccctacga ccacccgtcg gacatcatgg acgagatcgc ccgcctcacc cccagtttca
    32821 gcggcgtcac ctacgagctg ctggagcgcc gcggcagcgt gcagtggccc tgcaacgccg
    32881 atgcgccgga gggcacgccg acgatgcacg tggaccgctt catccgcggc cagggccgct
    32941 tcatgctcac gccctacgtc cccacctccg aacgggtcac ccgccgcttc ccgctgctcc
    33001 tcaccaccgg tcgcatcctc agccagtaca acgtgggcgc ccagacacgg cgcacgccca
    33061 acgtggcctg gcacggcgag gaccgcctcg agatccatcc cacggacgcg gaagaccggg
    33121 gcatccgcga cggcgactgg gtcggcatcg ccagtcgcgc cggcgagacg gtgctgcgcg
    33181 ccaccctcac cgagcgggta cagcccggcg tggtctatac caccttccac ttccctgaat
    33241 cgggcgccaa cgtgatcacc acggacaact ccgactgggc caccaactgc cccgaataca
    33301 aggtcaccgc cgtgcaggtc accctgtgcg cccagccctc cgactggcaa cagcgcttcg
    33361 ccctgcgcca ccatcgccag cgcggcctgc aggccaccgc cacgggaggc atcccccatg
    33421 aatgaggcca agcttgtcaa catggccaac cagatcgcca cggctgtcgc cgccaacgcc
    33481 gagccggccc gcgccgccat cgacgtggcc gagcatctca tgcgcttctg ggatccccgc
    33541 atgcgcgcgg ggctgatcgc ctgcgccggc ggcgcggccg gactcgaccc gctggtgcgg
    33601 gaagccgtcg cccggctgcg ggatcaggcc caggccaccg cctgaccgga tcgaccgcgc
    33661 tccgggctgc gcggcgggcg tacctgcgcc ggtaccacgc cgtgccgacg cagcaagctt
    33721 ccgagcagct ccacccgacg tccatgtacg cggatcagcc cttcatcgga cagttcgcga
    33781 aagatccgtg acagcgtctc cggcgtaaag cccaggcggg ccgcgagcag atacttcgga
    33841 atctccagat cgaggctgac gctgtcgccc caaccggcgg ccggagccac cgcgcgcagc
    33901 agatagtcca ccaccttctc gcagccggac tggaagcagt ccgccgcgat gtcccgttcg
    33961 atggccgtct ggcggtcggc gacggtctgc agcaggcgca tggaaagccg cgcgtcgtat
    34021 tcgatggccc gcatgacgcc ctcgcgacca atcaccagca ccacggacgc ccccaccgtc
    34081 tccgccgatg acacgtgggg cgcctgtccg aacagttcgg ccaccccgaa agcctggggc
    34141 gcatggacga tgtcgatgac cttctcgccc ccctccgcag acgttaccgc cactttcacc
    34201 tgccccgaca gcaagtaata tagggcgcgc ggcagctcgc cggtggcgta gatcccgcgg
    34261 cccttgggca gcgtggccac atgaccggcc cgcgacagtt ccgccaccgc ccggggatcc
    34321 agccccgcaa acagacgaat gcccggcagg acggccgaca actcgacagg actggtcttc
    34381 atggtttctc ccgcgtggat gtaatttttg gcttgcggtc cggccacgcc agcagcccga
    34441 ccatgcgcgc agtctaggca agcgccttcg cctcgatttg atttaagtca gaggcggcca
    34501 tgaagacatt ggcatcgact accccgaaag tgatacccga aattcacccg aaaatgttca
    34561 tcaacatgtt gttgtgtaac actattttta ctaacaccgg cacgcagccc agcgatctcc
    34621 gtacgcacat ggcgagcact ggcctatccc aaagtttccg atggaagcca ggccatgcgc
    34681 cctgcagcgg agacccggcg tgaggccccc tgacgcagcc gcctttgtca ccgctcgtgc
    34741 gcctcgacga ctttccgtcg tcgcgcgcct cggcgccctg atcgcggcca tcggcgcgct
    34801 ggccgtcgtg tccatcctgg cctccatgac cgtcgctgag ctggcctcag gccaggcccg
    34861 tgcgatcaac ctcgccggct ccctgcgcat gcagtcctat gtcatcgccg ccaccctcgc
    34921 cgacggcgaa cacggcgcgc tgctcgagcg ggcgctcagc ggattcgatg aacgctatgc
    34981 cgcccaggcg ctcacgaccg ccctgccggc atccgccgag gcgtccatcc gactcgccta
    35041 cgacgacgtc ggactgttct gggcccagac ctttcgcccg tatgctgaag ccgccccaga
    35101 gcctgaccgg gacgcgcgca atcgcctgac cgccgatctg gtggcgcgca tcgatcatct
    35161 ggtcgcgctg atcgagcaca atctggaatc ccgcctgcag accctgcgcc tcgtccaggg
    35221 catctccctc ttcctgctgc tcgtactggg cggtgtggtg gtccatcaga tcaaccgcca
    35281 ggtacgcgcg cccctcggtg aattgctgac ctgcgcccac gtcgtacgac gcggcgactt
    35341 ttcggttcgc gccgcccctc ggccgccgga cgaactcggc cagcttggcg aagccttcaa
    35401 ctacatggtg gaggatctgt cccgcagtta ctcccagctg gagacgcggg tcgaggaaaa
    35461 gacccgggaa ctggcccgca gcaaccagtc gctcgaactg ctctatcgca cgacccgcgc
    35521 gctgtccgag gccgacctca cacaggacag cctgttgaag gtgctgaacg aggtcgaacg
    35581 actggccggc gttcgggctg gcgccctgtg cgcggatccc ggtgacgggc gcccgctcgt
    35641 cgtgctggcc accgacatgg acaccgacct cgtcgcccgt ctgcctctgg acgggccgcc
    35701 atcggccgcc ccggccgcgt tgcaggtgct acctgccctc tcctacgcag gcgacggcgc
    35761 actcatcacc gtccccgtct cagacggcgt ccgcagcctg ggccgcctgt tcctgcagct
    35821 ccctgacagt gccccgccgg ccgagtggca gacaccgctg ctcgaaaccg tgggacgcca
    35881 tgtgggcgca gccctcgcca acgcccgccg ccatgaggaa cgtcaccgcc tcgcgctcct
    35941 ggacgagcgg gcggtgatcg cacgggaact ccacgactcc ctggcccagt ccctgtccta
    36001 cctcaagatc caggtgaccc gcctgcagaa ccttctcgcc cgccagtccg tctccgaccc
    36061 ggtgggcgat gtggtgcgcg agctgcgcga cggcctcaac gaggcctacc gacagttgcg
    36121 cgaactcctc accaccttcc gcctgcgtat cgacggacgc ggcctcaccg cagccatcga
    36181 cgacagcgtc cgggaattca gccaacgggg cggcctcgcg atccatctga gcaatcgcat
    36241 tgccggcctg gaactgcccg ccggccgcga gatccacgtc ctgcagatca tccgcgaagc
    36301 cctctccaac atcgagcggc acgcccgcgc ccacaccgtg gacatcgtgc tcgagcgcct
    36361 cgacgccggc gacctgcgcg tgtgcatcga cgacgacggc gtcggcatcg gcgaacagcc
    36421 ggcaccgccg agacgcttcg gcattgccat catgcacgac cgcgcccgca gtctcgatgg
    36481 cgcgctcacc gtgacggccc gtacggatcg tggcacccgc gtggagctgt gttttccggc
    36541 cgaaccaccc gccccaccct caccccgtgg aatttcccca tgaccgccac cgaagcaccc
    36601 agcactgtca tcgtgatcga cgatcatccc ctgttccgca agggggtatg ccagctcatc
    36661 gccctgtccg accgcctgcg cctcgtgggc gaagcctcca gcggagaaga cggactcacg
    36721 cttgcccaga cggccgatcc ggatctgatc ctgctcgatc tgaacatgaa gggcatgagc
    36781 ggcatcgaca ccctgcgcgc cttgcgggac gccgacctgg gcgcacgcat catcatcctg
    36841 accgtatccg atgcagccga ggacctggtc gccgccatcc gcgccggcgc cgatggctat
    36901 ctgctgaagg acacggagcc ggaagatctc ctcgcacgga tgtcggaagc cctcgccggc
    36961 cggatcgtca tcgacgacag cctgaccggc ctgctcgccc gttccctgcg cgaagaggcg
    37021 caatcagccg aacgcagcct cgctcatctc acggaacggg agcgcgccat cctccagtgt
    37081 ctcgccaccg gactctcgaa caagctcatc ggccgggaac tcggcatcgc cgaaggtacc
    37141 gtcaaggtcc atatcaagag tctgctcagg aaactcaagt tccgttcgcg cctggaagcc
    37201 gctgtgtggg ccattgagca ggacacgcgc tgaccctcca ccttcctgcc gacacggctg
    37261 cgtctcaccg ctcatgtgtt ccggcgggaa ccgacggaca tgtggcggag cgtccaccca
    37321 tccgcgcccc gcgagcgaca cccccacccc gattgcatcg gctggcgcgc ctcgaaccgg
    37381 caggcggatt tcaccatcgc cgcacaagag ggcgccacgc gccccgcgac ggcatccggt
    37441 gcccggttcc tgccccattt gcgtagccgg tttgagctgc gggtggcaca ggactactgc
    37501 gcactgcgcg gtacgccggc ctccacgatg tgccgggcgc cgcgctcccg cgccggcccg
    37561 gacccaagcc ggatcaccca ccccaccagg cgaagattca ccagccgggg cttggtgctg
    37621 gagatggcga tggaaggcgc acagcgcgcc tgtaccgccc gctgcccgcg ccgcagacgc
    37681 cccttttgcc tgccccggag cggacggtgg cgcagaaatg acaaaggccc ctttcggggc
    37741 ccaggtcagt gtggcggaga gtgagggatt cgaaccctcg atacgagttt tggcccgtat
    37801 gctcccttag caggggagtg ccttcgacct ctcggccaac tctccacacg agcccggcat
    37861 tatagatacg ccaggcgtgt cggtcaaatc acgcagcggc ctgatccaga ccgaatgcac
    37921 gatgcagcac ccgcacggcc agctccaggt acttctcgtc gatggcgacg gagatcttga
    37981 tctcggaggt ggagatcatc tggatgttga tgccttcttc agccagggtg cggaacatgc
    38041 gggatgcgac gcccgggtgg gagcgcatgc cgacgccaac gatggagacc ttggccatgg
    38101 tgcgatcacc cttgatctcg cgggcgccaa cgtgggcctg gacgtcctga aggatcttga
    38161 ccgtcttgtc gtaatcactg cgattgacgg tgaaggagaa atccgtggtg ccgtcgtgac
    38221 cgacattctg gatgatcatg tccacatcca cgttggcatc cgcaacgggc ccgaggatct
    38281 ggtaggcaat gccgggcttg tcagggacgc cgagcacggt gagcttggct tcgtcgcggt
    38341 tgaaggcaat gccggagatg atcggctgtt ccatgttctt gtcttcctca acggtaatca
    38401 gggtgccttc gccttcctcc tggaagctgg acaggacccg aagcttgact ttgtacttgc
    38461 cggcgaattc caccgaacgg atctggagca ccttggagcc caggctggcc atctcgagca
    38521 tttcctcgaa ggtgatggtg tccagcttgc gggcctccgg cacgatgcgc ggatcggtgg
    38581 tgtaaacccc atccacgtcc gtatagatct ggcactcgtc cgccttgagc gccgccgcca
    38641 gggccacgcc tgtggtatcg gagccgcctc gcccgagggt ggtgatgttg ccttccgcat
    38701 cgacgccctg gaagcccgcc accaccacca cgcgaccggc atcgagatca cgacgcatgt
    38761 tgtcgccgtc gatgtcgagg atgcgggcct tggtgaagga actgtcggtg aggatgcgca
    38821 cctgaccgcc ggtgtagctg cgcgccggaa cgcccacttc ttccagcgcc atgcagagca
    38881 ggccgatggt gacctgctcg ccggtggaca cgaccacatc gagctcacgg gggctcggac
    38941 tgccgctgat ttccttggcc agggcgatca gccgattcgt ctccccgctc atcgcggaga
    39001 ggaccacgac ggtctggtgc ccctgggcat gaaacttcgc gacccgcttg gcaacgttct
    39061 tgatccgctc gggatttccg acggaggtgc cgccatactt ttgaactatg agcgccatct
    39121 tgacttctaa gagtgaaggg tgtggaaaac atgcaatctt agcggatcag agcagttcgt
    39181 gcagcttcct catgcgtcat gcccctgctt cccgtctgaa ccgagatcgg caaaaatccc
    39241 gtcatgaagg gtgtggatcc tgtcacaaaa aaaacggtca gatttctcta atcgaacaga
    39301 atggggcgtt gtatgtcctc cgcgtagtaa tctatacttt ctgacatata acgaaagtta
    39361 tatcagcaaa actcctgatg cgctgctcaa actgacagag tgacttatct aagaaggatg
    39421 gcaacatgaa gacgaatgag aaggtggacg tgcgcgaaat ccgtcgcaag cttggcatga
    39481 accagtccca gttctggtcc aaaatcggcg tgacgcaaag tggcggctcc cgctacgaga
    39541 gtggtcgcaa cattccccgc cccgtccagg ccctgctccg cctcgtgcat atcgagcagg
    39601 tggacatcaa caagatcaag aaggatgacg tggaagttgt tgaatatctt aaggccacga
    39661 atcccgagct gttcaagacg ctcaaaaagg aagcccgcgc caagcgcaag gaacgtgttc
    39721 ctcactgagc ggacgcgccg cttacgggct gacattcacg gtcagcccgg ttttccggag
    39781 ggcgacggca atgtgctcga gttcttcggc actcaggggc tgaaggtgga tggcttcagg
    39841 ctggtgcgat tgccgtacga cgccataaag caggatgcct ttaagcccct cgagtccggc
    39901 tttttgcaga aatgcgacat atgaatctga aaattcttgt gtcggcaatt cgccatccca
    39961 ccggaaaaca cacgtctgta cccatgttgg gcaaatcgaa gccgtcaatg ccagacgacg
    40021 cgcgtgcgcg gccggatcaa tatccacgcc gttgatcttc agaatctctt cccgtgtacc
    40081 ggcatcgacc ttgaaccaca cctcgccccc cgcttcggcg agcgtcctga aggccgcctg
    40141 aacggaaggg cgatccgcga gactgccatt cgtgatgacc cggatcggca cattcctcag
    40201 accgcacgcg ttccggattc cgacaacgcg ttccatgatg tcggcaaatg ccttgcagct
    40261 tgtcggctcc ccatttcccg aaaaggcaat gtcgcggatc accctcatct cttccgggac
    40321 atgatccttc atgaattggc cctgctgcag ttctgtcagg aaaccctgca attccgccgc
    40381 cagcagctcg atatcaatgg acggggccgc cccccgaacc agccctggca cctggcaata
    40441 cgcgcaatgc cagttgcacg cattgttggg attgaggttg atccccaccg acacgccccg
    40501 tgcccgccgt gacaccacgg gatagacgta ggtcagcgca ctgaagctgc ggttgtgatc
    40561 ggcgacggtg agcatctgcg aggaagcggt catggaagat ccggtgcggg aaacgagctg
    40621 cgtataatac gagacctttt ttcccgtccg ccgttcccat gcgaatcacg cgccgcctgg
    40681 aattcgatgc aggccaccgc attcccgacc acgccagcca gtgccgccac ctccacggcc
    40741 accgctatgc cctcgagatc accctgtcgg gcgacgtcgt ccgggccgac ggtgcgccga
    40801 tcaacggcat ggtcatggac tttgccgacg tcaaggccat cgccaaggaa catgtcgtcg
    40861 atgtgtggga ccacgccttc ctggcctatc gccacgacac ggccgtggtg ggcttcctcc
    40921 agtccatgcc cggacacaag acggtcatcc tcgacgtcgt ccccaccgcc gagaatctgg
    40981 ctgccgaggc ctttcgcatt cttgatccac tctaccgcga cgtgtacggc aaccacctgc
    41041 gccttgagcg catccgcctc tacgaaacgc ccaactgctg ggccgacgcc ctgcgggact
    41101 gaaccccgac accacgcctc acacgtaacg tacccgcagc cgcccgacac cctccacccc
    41161 ggcctccacc acgtcgccgg ccaccacggg ccctacgccg tccggtgtgc cggtgaagac
    41221 caggtcgccc ggctccagcc ggaaataacg cgacaggtgc gcgacgattt ccgccaccga
    41281 ccagatcatg tcggcaatgt cggcccgctg acgtgactcg ccgttccgct ccagccagat
    41341 cgccgcccgc tgcgggtgac cgatcgcccc ggccggcacc agcgtcgaca ggggcgccga
    41401 gcggtcgaag gccttgccca gctcccacgg ccgccccttg tccttgagcg ccgtctgcag
    41461 atcccgccgc gtcatgtcga gcccgacgcc atacccccac acgtgggcca gcgcctcgtc
    41521 gacaggaatg tccgcgcccc ccaggccgat ggccaccacc aattcggcct cgtaatgcag
    41581 gttggccgtg gccacgggat agggcagctc cagcacccct tccggtccca cggaaaccac
    41641 cgcgtcggcc ggtttgcaga agaagaacgg cgcctcacgc gtcggatccg cccccatttc
    41701 gcgggcgtgg gccgcgtaat tgcgtcccac gcaatacacc cgacgcactg gaaacagccc
    41761 gtcccccaac actggcacca ccaccggtct gggcggttcg atcacatacg ccacgacaca
    41821 cgctccttac gtcaatcctg ccgtctgcgc cgtaaccggc gcccgaatga gcgccgcacg
    41881 cccgctccgg tggtaacccg ctaccaccca cacgcccgac cctctcgcca cactttaacc
    41941 tcccacaccc ccacggagac tttcatgacc aagaaaatcc tgatgatctg cggcgacttc
    42001 tgcgaagact acgaaaccat ggtgccgttc caggccctgc tggcggtcgg ccatcgcgtg
    42061 gacgccgcct gcccgggcaa gaaggccggc gacaccatcg ccactgccat ccacgacttc
    42121 gaaggcgacc agacctacac cgagaagcgc ggccacgcct tcgccctcaa cgccaccttc
    42181 gccgagctgc gccccgaaga ctacgacgcc ctgctgattc ccggtggtcg cggtccggag
    42241 tacctgcgca cctacccgac ggtgatcgcc gccgtgcgcc acttcttcga ggccggcaag
    42301 cccgtcgccg ccatctgcca tggtgcccaa ctcctcgccg gcgcccgcgt gctggaaggc
    42361 cgcacctgct ccgcctatcc cgcctgccgc gctgaagtgg aactggcggg cggcacctat
    42421 gccgacatcc ccatcgacca ggccgtcacc gacggtaatc tggtcacggc acccgcctgg
    42481 cccgcccacc cggcgtggat cgcccagttc ctcgccgtac tgggcacacg catcagcctc
    42541 tgaggcgcgg cgcctccgga ccgcctacaa tggccagacg ggggcctgcc cgtgtgcctg
    42601 gatgcgcgac caccatgtgc cagatcttca tcaacgccga ccccgacctc tacgccaccc
    42661 gcagccgctc cctgcgcctg catggcgtgg ccaccagcct gcgccttgaa aacctgttct
    42721 ggcaggtgct tgaagaaatc ggtgcccgtg acggcctcac ggtgccccag ctgaccagcc
    42781 gactctacga cgaactcctg cagagcggcc gcctcgacga cagcaccaac ttcgcctcct
    42841 tcctgcgcgt ctgctgcctg cgctacctgc gcctgcaact cagcggccgc atccccgccg
    42901 acacggccgt gccaatccgc gaactggacc ccgcctgggt gctgaccgcc gagcgccacc
    42961 cctgcgcctg aggccacccg ccatgatcga cctgcactac tggaccaccc ccaacggcca
    43021 caagatcacc ctgttcctgg aggaaaccgg cctgccctac cgcatcgtgc cggtgaacat
    43081 cgggcgcggc gagcagttcc agccagactt cctgcgcatc gcccccaaca accgtattcc
    43141 ggccatcgtc gaccacgccc cggccgatgg cggcgcgccc gtgtcggtct ttgaatctgg
    43201 tgccatcctc ctttacctgg ccgaaaagac cgggcgcttt ctctccaccg aactgcgcgt
    43261 gcgcctcgac accctgcaat ggctgttctg gcagatgggc ggactcggtc ccatggcggg
    43321 ccagaaccac cacttcgtgc agtacgcccc cgaacgcatc ccctacgcca tcgaacgcta
    43381 cgtcaaggag accgcccgcc tctacgccgt gctcgaccgc cgtctggccg accgggactt
    43441 catcgcgggc gcagactatt cgatcgccga tatggcctgc tacccgtgga tcgtgcccca
    43501 cgaccgccag caacaggacc tcgcgcactt tccccacctc gcccgctggt tcgacgccat
    43561 ccgggcacga cccgccaccg tgcgcgccta cgccctggcc gagcgcatcc acgccgcccc
    43621 cgtggtggat gatgccgccc gccaggtcct gttcggacag gacgccggca ccgtgaaacg
    43681 cccctagccc gcggcacggg cgggccgatt ccggtagaat gcccgcctcc ctgaagctgc
    43741 ctccgccctg ggcccgggac tgcttccccc tccacccggc ccgcgcctcc cgccgtccat
    43801 gactgacgcc atcgaacgct tcgaccagct cgacctgcaa ccggcgatcc tcgccgctct
    43861 ggccgagatc gggtacgaaa ccccttcgcc catccaggcc gcctgcattc cccacctcct
    43921 cgcaggccag gacatcctcg gcgaagccca gaccggcacc ggcaagaccg ccgccttcgc
    43981 cctgccggcc ctcgaccggg tggacctcgc ccgccgcgaa ccccaggtgc tggtcctcac
    44041 gcccacccgc gagctggcca tccaggtggc cgaggccttc cagcgctacg ccagccggat
    44101 ggccggcttc cacgtgctgc ccctttacgg cggccagagc atggtcgtgc agttgcgcca
    44161 gctccagcgc ggcgcccacg tcatcgtcgg cacgccgggc cgcatcatgg accacatcga
    44221 gcgcaagagc ctcaacctgg acggcctcgc caccctgatc ctcgacgaag ccgacgaaat
    44281 gctgcgcatg ggctttatcg acgacgtgga gtggatcctc gaacacaccc ccgccgagcg
    44341 gcagaccgcc ctgttttccg ccacgatgcc ggacgccatc cggcgggtcg cccaccgcta
    44401 tctgcgcgaa ccccgcgagg tgaagatcaa ggccgccacg gcaaccgtct ccaccatcca
    44461 ccagcgctac ctgcagatcg ctggcgccca caagctcgac gccctgaccc gcatcctcga
    44521 agtggaagac gacttcgacg ccgccatcgt cttcgtgcgc accaagaccg ccaccgtgga
    44581 gctcgccgaa aagcttgaag cccgcggtta cgccgctgcc tgcctcaacg gcgacatgac
    44641 ccagcagctg cgcgagcggg tcatcgagca gctcaaggcc gaaaccctcg acatcgtcgt
    44701 cgccaccgac gtggccgccc gcggcatcga tgtgccgcgc atcagccacg tcatcaatta
    44761 cgacatcccc tacgacaccg aagcctacgt ccatcgcatc ggccgcactg gccgcgccgg
    44821 gcgcaccggc aatgccatcc tctttgtcgc cccacgggaa aaccacctcc tgcgccagat
    44881 cgaacgtgcc acccgccagc ctatcgaagc gctcaagctg cccagccgcg aggccgtcgc
    44941 cgacaagcgc gtcgccgcct tccgccagca ggtggccgaa gttctggcgt ccgaggaact
    45001 ggacttcttc ctcgacgtca tcgccggcat cgaggacgca cacgacgccg aaatccacga
    45061 gatcgccgcc gccctcgcct tcctggccca gaaggaacgc ccgctgcaga tgcccgacaa
    45121 cggccagccc gacatcgccc agtttgccgg cagcacccag cgcgaacgtc cggcccgcga
    45181 aaaccgcggc gagatcctcg aacgtcgccg tgactacgcc gaagggcgcc tgatgcgcta
    45241 tcgcatcgac gtgggtcgcg aacacctggc cagcccccgc gacatcgtcg gcgcaatcgc
    45301 caacgaaggc ggcatcgaca gccgctacat cggccagatc aacctctacg aagactacag
    45361 caccgtcgaa ctgcccgcca atctaccgcc cgacgtggtg cagaccctgc gccgcgcccg
    45421 cgttcgccag caggccctca acctgcgccc ggccagcgcc gaggaatccg cgcgcccgcg
    45481 cggcgggcgt ccagccggaa agccgccccg caaggacggc cctggcggct tcaagccacg
    45541 caaaccccgc gcctgaagac tgaccgggcc accctgcccc ggcccggccg gggccgattc
    45601 agccgcgcca gcatcgggcg cccccgcgcg ccgcgtccag acctgtcaaa gtgtcgcgag
    45661 tgcgcgttgc ttcgccccca cagtcgcttg ctgacccggc ccgagcctat ccagctctgt
    45721 taaactgcaa catttcagtc atcacgttgc aataaacagc ccatgtttgg acgtctcctg
    45781 ccccaggaag ggcaattttt cgagtatttc aatgcccacg cggcgcagat cgtgctcggc
    45841 gcccaggaac tcgtcgcgct gatggaagac ctctccagcg ccgaccgtcg catctctgcc
    45901 atcgagggca tcgaacagag cgccgaccgc atcacccacg acaccattgc ccagctccac
    45961 aagagcttca tcacgcccct cgaccgggac gagatccaca gtctcatcaa tgcgatggac
    46021 gacatcctcg acaccgtcca ggacgtggcc gagtgcacca ccctctacca cctccagcgc
    46081 agcacgccgg aggctgtcca gctcgccaac atcagcctgt cctgctgcac ccgcgtcaag
    46141 atcatcgtcg ggctcatctc caacatggac aacgccagcg ccatcctgca gacctgccag
    46201 gaaatcgacc gcctcgagac cgatgccgac cgcatcatgc gcgccgccat gtcgcgcctg
    46261 ttccgggatg agcgggacgc cctccaggtc atgaagctca aggccatcta cgagcagctc
    46321 gaatccatca ccgaccgctg cgaggacgtc gccaagatcg tcgaaggcat cgtgctcgaa
    46381 aacgcctgag tccgtcatgg aatccctgca tatcagcctc tgggtggtca ccgggctggt
    46441 ggtcgtggcg ctgctctttg atttcatgaa cggcttccac gacgccgcca acgccatcgc
    46501 gacggtcgtc tccaccggcg tgctcaagcc ccaccaggcc gtcgcctggg ccgccttctt
    46561 caacttcgtc gccctcttcg tgttccacct caaggtggcc agcaccgtcg gaaagggcat
    46621 cgtcgatccc gccgcggtcg atcatttcct gatcttcggc gccctcatcg gcgccatcgc
    46681 ctggaacgtc atcacctggt actacggcat cccgtcgagc tcgtcccacg ccctcatcgg
    46741 cggactggtc ggcgccgcca tggccaaggc cggcactgcg ccgctgatct cggcgggcat
    46801 ctggaagacc gtcgcattca tcctcgtctc acccctggtg ggctacctgc tcggctcgct
    46861 gatgctggtc atcgtcgcct ggatctgccg gcacaagccc cagcgccggg tggacaaatg
    46921 gttccgccgc ctgcaactcg tctccgcggc gctgtacagc ctgggacacg gcggcaacga
    46981 cgcccagaag accgtgggca tcatctggat gctcctcatc gccgccggcg tcaccgccca
    47041 gggtgatccg gtgcccatgt gggtggttgt cctgtgttac ctcgccattg gcctcggcac
    47101 cctgttcggc ggctggcgca tcgtcaagac catgggccag cgcctcacca agctcaaacc
    47161 ggtgggcggc ttctgcgccg agaccggcgg tgcgctgacc ctgttcatgg cgtccagcct
    47221 gggcatcccc gtatccacca cccacaccat caccggcgcc atcgtcggcg tgggctcggt
    47281 acgccgaacc gccgccgtgc gctggggcct cgccggcacc atcgtgtggg cctgggtcct
    47341 caccatcccg tgctcggccc tgatcgccgc actggcttac tgggcagggc ttcagatcat
    47401 gtgacggtcc gcttgacgcg cgcggggaat catccgatcc tcgcgcccgt ccgaatctgc
    47461 agatctccct cgatggcgcc tccgaccctc aaacagcaaa agaccttcgc cctcatccgc
    47521 atcattggcg gcatggcttc cgccggcgtg ctcggctatt ccttcgtcag caacgtcctg
    47581 gcgggagccc ccgccgaagg cgccctcctg gcgacgggcc tgatggcctt cctcgcgctg
    47641 gcctacgccg gctattacac ccgcgtcctc ggccgcgtcg cccaagccga gcaacaggct
    47701 gccgacaagc cttgaagcaa cgcaggcttc tcgccgcctg gaccttggcc gccacactgg
    47761 ccggatgcgc caccaccggc ccgctggaat cccgaccttc gcccaccctg ccgccggcgc
    47821 cgggttcgcc cactccgcgg gcaccggccg acatagatcg cccacgccct gtctcgcccg
    47881 cgcctgctcc aattggcacc gcccccacgc cacccgcacc gcccccgata ccgagcgccc
    47941 aggacggtcg cgccctgatc gcccgcttcc tgccgccggg cgtcagtgac cgggagggct
    48001 gggcgaccga cctccacacc gccttcacgg ccctgcatct gtccctcacg ccggacacga
    48061 tctgcgccgc ggttgccgtc atcgaacagg agtccagctt ccaggccgac cccgcggtgc
    48121 ctggcctgcc caagatcgtc tggcgggaac tcgaagcacg tcgccagcgc tatcaccttc
    48181 ccaggctgat gctcgacgcc gcactcctcg ccccctcccc ggacggccgc agctaccgag
    48241 cacgcatcga cgccctacgc accgaaaaac agatgagcgc cctgttcgag gagatgatct
    48301 ccgagtttcc ccagggcaag acgctcttcg ccgattacaa ccccgtgcgc accggcggcc
    48361 cgatgcaggt gagcatcgcc ttcgcccagg accagatccg cgagaagccc tacccgtatc
    48421 cccgtcgtgg cagcgtgcgc gacgaagtct tcacgcgccg cggcggcgtg tatttcgggg
    48481 tggccatcct gctcgattac ccagcgccct acagccgcct cctgtaccgc ttcgccgact
    48541 tcaacgcggg tcgctacagc agccgcaatg cagccttcca aaacgccgtg gcgcgcctct
    48601 ccggccaagc gctgacgccg gacggcgacc tcctgcgtta tcgcgacgga gcgccctcgt
    48661 ccgagcccag cgccacctac tcggcgctga gcacgctcgg cggccgcctc ggcctgagtc
    48721 cggcggagat cgcccgcgac ctgcgcctcg agaaaacctc ccagtttgcc caaaccccgc
    48781 tctacattcg tatctttacc ctggccgaca gtctcgacgg tcgtcgccag ccccgcgaaa
    48841 tcctgcccga gatcgacctg aagagcccca agatccagcg caagctgacc accgcctggt
    48901 tcgccgaaag ggtcgatgcc cgtcaccgca cctgccttgg ccgcgaccgc tccttcgccc
    48961 cgtgaatccc ttgtcccacg ggggctttcc ttgatccaga ccaagcgccg gagccccctg
    49021 cggatcgtac aatcgagtac aaatcacctc taaaagacgt ggtttaatct cgaaaaaatt
    49081 cacgaccaag ggagaacaac accatgactg tgcaggaaca atacgccgcc aagcgcatga
    49141 gcgccgcgga ggccattcgt cacgtccaca atggcgacac catcatcgtg ccgaccgccg
    49201 tggccgaacc gcccgccctg ctcaacgccc tctcggagca ccgtcgcgat ttccacgacg
    49261 tgaaggtttc ccagatcctc gcggtacgca agatgggtta cctggacccg accaccgccc
    49321 agcacgtgcg tcacgtcgcc tacttcttca gcggcgccac ccggccgggc ttccacgaag
    49381 atgtggtgga cttccgcccc agctatttct ccgaaatgcc gcagctcatc gaacgcggcc
    49441 tgatgcctgc cgaagtcgtc ttcacgcagg cctcgccgat ggacgcacac ggttacttct
    49501 ccctcggcct cgccgccgac tacacgatgg cggccatcag caaggcccgc gcggtgatcc
    49561 tcgaggtcaa cgagaacgtc ccctacgcct tcggcaacaa ccaggtgcac atctcccagg
    49621 tcaccgccct ggtcgagaac aatgaaccgg tgctcgaggt cggtctgccc gccatcggcc
    49681 cggtgcagga agccatcggc aagtacgtgg ccgacatgat cgaagacggc tccaccctgc
    49741 agatcggcta tggcggcatc cccgacgccg tggtgatgca gctcacccac aagcacgacc
    49801 tgggcattca caccgaaatg atcggcgacg gcatcctgac gctaatcgag tcgggcgccg
    49861 tcaccaaccg ccgcaagaac tacctgcccg gcaagatggt cgctaccttt gccctcggct
    49921 ccaagaagct ctacaactgg atcgaccgca acccggcctt cgagatgcat ccggtggact
    49981 tcaccaacga tccctacctg gccggcctca acgacaacct ggtgtccatc aacgccacgc
    50041 tggagatcga cttcctcggc cagtgcggtt ccgagagcct gggcccgatt ccctactccg
    50101 gcaccggcgg ccagtccgat ttcgtccgtg cggccaaccg ctccaagggc ggcaagtcct
    50161 tcatcgtgct gccctccacg gccaagaacg acaccatctc gcgcatcgtg ccgaccctca
    50221 cgccgggcac ccacgtgtcc accagcaaga acgacatcaa ctacgtcgtg accgagttcg
    50281 gtgtcgccca gctgcgcggc aagaccgcca aggaacgtac ccgggcgctg atcagcattg
    50341 cgcatcccaa gttccgcgac gagctcaccg agaccgccaa gaagatgcac ctgctgtaag
    50401 ccggcccacc gccgcgcccc cgccggggcg gtgcggcagc ggtgcccgtt ccggggcccg
    50461 tcacggttct gccgtgccgg gccccggtgc gttcacggga tctgcgactc caggacgaag
    50521 tccgcaccgt catcctgggc cagcacatcc ttgagccacg gcagtaccgt cttcagcgtc
    50581 tccggcaggg tccacggcgg attgatcacg aagagcccgc tgccgtgcat tccgaagccg
    50641 tcctcggatg gcttcttcac gtccaggctc acgtgcagcc agctgtcgag gggcaggcgc
    50701 ttgagcttct cgggcagctc gcgggcgtgc attgtggtaa ggcgcgggta ccacaccaca
    50761 tacgtacccg tcgcaaaacg ccgcagacac tccttgagcg ccgtcagcac ggtacggtaa
    50821 tcggtcttca cctcgtaggg cggatcgatc aacaccagtg cccgccgcga cggtggcggc
    50881 aggaaggcat tgatgccggt gaagccgtcc tcctccttga tcatcacctt gctgcccgcc
    50941 tcggcgaagg tctggtgcag caggcggttg tctgccggat gcagttcgaa caggcgcatg
    51001 cggtcctgct cccgcagcag cggcgccgcg cagcacggcg agcccggata gaggcgcagc
    51061 cggcccggcg gattgagatc ccgcacgatg cgcagatagc tcttcagctc ggccggcacg
    51121 tccttgcgcg ccagcagccg ggccacgccg tcctcgaact cggcgttctt gcgcgcaaag
    51181 gcctcttcca gcccgtagca gccggccccc gcgtgggtgt cgatgtacag atagggcttg
    51241 tccttgcggt tgaagtattc cagcaactgg acgaggacga agtgcttgag cacgtcggcg
    51301 tggttgccgg cgtgaaaggc gtggcggtag ctcagcatgg caggcgtggc gccggtcggc
    51361 gcgggacggt caggggcgcg atgctatcac ctcggcccgc ctgtcgctgc gccgtgtacg
    51421 tcactccggc gcgttgtact tgcggcgcga gccgcggaag cgttccacgg ggtacttggc
    51481 ggcgtttttc tcgagcttgg ccagcgccgc cacgcccagg tcgattcctg cccggtcggc
    51541 gatgagcagc agatagagca gcacatccgc gcattcgtcc gccagtgcct cacgaccgac
    51601 cggatcggac ggcagtgcat cgatctccgc gtcgggtcgc cactggatca gctccagcag
    51661 ttcccccgcc tccagattga gggacacgat caggtggcgc agggcatgga actggcccca
    51721 gtcgcgctcg tcacgaaagg cctttacacg gcgctggagc gcgtccagat cactcatggc
    51781 gcgatcaccc tttgggacgg tcgagaaacg ccgccacggc cgcctcatgg tccgcagaac
    51841 cgtgggcaag ggcctggtaa gcggcagaca gttcgagcag tgactcgagg cgcagcgtct
    51901 gagcctcgcg catcagacgc ttggtcatgc gcagcgcgtg gccgggctgg gcggcgatgc
    51961 gctcggccag ggccatcgct gcgtcgagca gcacgtccgg ggccacgacc cgcgaaacca
    52021 gccccgcttc cagcgcctgg gccgcgtcga gccgatcacc ggtgaagctc atctccgccg
    52081 ccttggagta gcccaccacc cgctgcaaca gccaggcgcc cccatcgccg ggaatgatcc
    52141 cgaggcggac gaagctctcc gcaaagctcg ccgactcgga cgcaatgcgg atgtcagcca
    52201 tgcacgccag atcgcagcct gcgccaatgg ccgggccgtt caccgccgcg atggtcggta
    52261 catcgaggcg atggaaagcc agcgggatgc gctggatccc gctacggtaa ttctcgcgga
    52321 tgtccagccc accgcccctg gccagcccgc gcttgtcccg gaaatccttg acattaccgc
    52381 cggcacagaa ggcgcttccc gccccggtga ggatcacggc cttcaccgaa aggtcagcct
    52441 gaatacgctc acaggcgtcg accagcgcct cccattcggc cggagacgac agcgcgttgc
    52501 gggtttccgg cgcattgagg gtcagcgtca cgatgctgtc ccggcgctcg aaaaggacga
    52561 aatcactcat gggtcagact cccctgttgt tggaattcga ttcgacacgt cggtcggcag
    52621 gcgggcccga agaggcgcct cccgatgcca tccagacggc ctaagccctt ctttccctta
    52681 cggaaagcgc ctgtaaagca cgattttccc cttgcacaca ttcgttaccc tgatattatg
    52741 gcgcgctctt cggtcggcct ttcggcggca cggccgagcc ccccgtacaa aaggaaaata
    52801 acatgccgat ttaccgtctc ggagagcgcc agccggagct gggagaaggt gtctggatcg
    52861 cggaaaacgc caccgtcatc ggatccgtgg tactgggtcg caacgtgaat gtgtggttcg
    52921 gctcgatcct gcggggcgac aacgacccga tcaccatcgg cgagaatacc aacattcagg
    52981 acgggtcagt gctgcacacc gacgacggtg tgcccctgac catcggacgc aatgtgaccg
    53041 tcgggcacaa ggtcatgctg cacggctgca cgatcggtga aggttcgctg atcggcatca
    53101 acgccgtcgt cctcaaccgc gcggtgatcg gcaagcactg cctgatcggc gccaattccc
    53161 tgatccccga aggcaaggtg attcccgacc gttcgctggt ctgcgggtcg ccagggcgca
    53221 tcatccgcga gctgaccgat cacgagatca gccgtcttca ggcgagcgcc gaaggctacg
    53281 tgaagaacgc ggaacgctac gtccaggaac tgatcaagct ctgagcggat gcggcaggga
    53341 ggggatcccc tgccccgccc tccggatgaa cgccgaggac agcgccttcg cgctgcccac
    53401 acatgggcgg cggacggttc ggcccgcgag gcatttacct gctcacgcct gcccggcgat
    53461 cagtcgagcg acaggacgat gggctgatgg tcggacgcgg ccgtcaattg gtccaccgca
    53521 acggcagcca cccgatccac cagatcggcg ctcacgaaga aataatcgca gcagtacgcg
    53581 tgatccggcc attccgcgcc gttgagccct accgtgctga ggtgcggaat atccccgtag
    53641 caggcccgcc aagcgtcttc ccagcctgac acgtccggtc cgatgggggc gatcatccgg
    53701 aaatactccg gactgcccgg ctcgcagttg aaatctccgc agatcaccgc cgacgccgga
    53761 cgcggcaaca cctcgaaggg cgatcccggc gccgcattgc ggcccggcgt gtcacccccc
    53821 gcgttggcga gtctgacggc gtcctgttgc agtgtgtgca acgcggctat ctgggtcatg
    53881 cgctggacgg ccgaataata ttcgagatga gtgttcatca cccgcaccca gccctgcggg
    53941 ccttccacca ccgtttcgag acagacccgc ggcatgctcg gcatgcccgg ctcgggcgga
    54001 cgtggcaggc tatggcggaa gacctgcccg acaggcaggc gcgacagggt cagattgcca
    54061 aatcggctgc gaccgccgtt tccgtcgggc atgtccacac cggatgcaaa gtggaccgaa
    54121 tagtccggga acgctgccgc gatgctggcc acgccatcca ccggaacgcc gccctggagg
    54181 gcaggaaagg ccacggccac ttcctgcaga cagatgacgt caaattcacc cagggtacgt
    54241 accgccgaca cggtgcgctc cagcgtcacc atgccatcgg ccccgcgccc ccactgtatg
    54301 ttccagctca atatcttcat cgggtcctcg gttcggtcat gcatcggaca ccgtattccg
    54361 gcatccgaga atcatcaaaa tcgagaatat caagcaatct gaaagccgtc cgcagcaaaa
    54421 gcggacgcgc cgtgtgcaat tgccggaagc acgtcaagat gcggacgaaa ccctgtcgta
    54481 tttcacgccc agagcccccc ggaaggcccg ctccgcgcct gcaagggtct aaagccctgc
    54541 accgccgcag ccgtaagtag ggcattcccc tcacccccgg cgccatcatg ataacccccc
    54601 aggaacttgt cgcctgcatc gaaaaactga ccagtctgcc cgcggtgtac caccgcatcc
    54661 gtgcgctcct cgacgatccc gatagttccg tgcacgatct cgccgaagcg gtgtcgagcg
    54721 acccggcgat cacggcacgc gtgttgcggg tggtcaacag cgttcttttc ggctttcccg
    54781 ggcgtatcga gaccgttgga agggccgtga acctgcttgg catgcagcag gttcacgatc
    54841 tggtgctcgg cacggccatc gtgggcgcct tcaatggaat ccgcccggca cgcatgcata
    54901 tgagccgctt ctggaaggac agcgtctatc ggggtctggc cgcccgcgcg gcagcccatc
    54961 ggctgggcct gggggatccg gagcggatgt tcgtggaagg tctgctggcc gacatcggcc
    55021 atctggtcat gttccaggcc gcgccggata tggcagatgc cgcgctggag cgcgcgcaca
    55081 gacaccacat tccgatccac gaggccgaac agtccgtcgt gggctgtcat tacgcggaag
    55141 tcggcgctgc gctggcctcc gcctggcacc tgccagccgg attttccgcc gcaatcggcg
    55201 cccagctcat gcctctgctc gccggtcccc acactcagga agccgccatc ctgcacctgg
    55261 ccaatgatct gctggccagc gacgccgacg aggtggacga ggacgccgtg ttggccacca
    55321 tatcgccgtc aatcgtcacc atcctgcagg cggatgcctc gatgctgggc gcagtgcgcg
    55381 cccgggtgcg ggacgaactc tctgccgtga tcgccctgtt ctttcccgat cagtcctgag
    55441 cgcctcgctc agcccttgcg aaattcggtt tcgaggacat cgcccggcga cagcgtgccc
    55501 cgccacgtca ccgtggttgc gggcacccgc cggtcataag tgcgggaaat ccaccccgca
    55561 aagggttgct cggacgcctg caggatgctc acgtccccgc cggacggcag gaccatgtca
    55621 acccgcacgg gcccgttatg gacggcaacc cccttggtgc tgcgggccac cagacagtct
    55681 tcgccaaaat gccagcacag ggtcaccagc tggggcatgg tggcgatgac ctcgtcccgc
    55741 acgctgagac tgtcggtctt ccggtcgaac gaccaggtcc gacgatgcac gggcttgccg
    55801 ctcaagcggc gatagccatc gtgttccgcc accagcacgt ccccgtcggc atcgctctcc
    55861 cagcggatga cgctcacggc gtacttgcgc ttccacagga aacgaccgcc agacacggcc
    55921 tgatcttcgc tgccgatcac cagcgtgttg tgcagggccg tactgcggaa ggcgtcgcgc
    55981 caggctcgct gaccgtgata ggtcccggat ccggggtcca ccagaaattc ccgccccgaa
    56041 acggacaggg tgaaggacag cgcgtcggcg tgcccgtgct cggccatcgc accggcgccg
    56101 agtgcccccg cgtcggcaac gatccgccac tccagcggcg tatccagatg cgcaccgagc
    56161 acatagacgc caccctcggg aaacgccgtg cggagggtgg acggaaaggc tttcaacagc
    56221 agcagatcga actgccgcgc ggcatcgacg cccatcaacc agcgcgtccg cggatcaagc
    56281 atgcccgcct tgcgggcgaa cgcgggccgc gagaacaaca aggcccccgt cgccagcatc
    56341 gagcggtacg cgcagccccc cgccgcctgc cccaggccaa gggcatcgcc gtcgtccgaa
    56401 tcgccaaact ggggtacgtt gcccccagta tccatcaccg ccgccacgta ttccgccatg
    56461 gcctcgaggc ggcacccgag ggcatgggag aactcccggc ccgttgcacg cgccgccagt
    56521 gcagcgatgg acaatacgtc ccaggtcatg cgctgggcgg ccatggagtg ctccagcacc
    56581 accccgtcgg cggaaatctg gcgcggaacc tcctgctcca gcccatcccg tccggtcgca
    56641 cgccagacct ttgcctggct ccagtacggc caagtcgcac cggccacata caagcccgcc
    56701 agttcaccga gacgatcgct ccccgccgag gccgggcgcg agagatgcgc ctggataaac
    56761 tcggcatggc gccacaccga acccagccag tcggaacgca ggaccttgcc caccggttcg
    56821 gcgaacaacg ccgaattggg gccgccaatg agttgccaca ccgtcgccca gttcatcaga
    56881 cgtaatgcca cctcgacggc gctcgaccag ttcggcccgt gggggtaggg actcttctcg
    56941 agccagtccg acatgagcac gccgatggca tccagatagt cgcgacaccc agtctttcgc
    57001 cacgcaaggc ccaacgatac gagctcccga tggcggttga gcgtccagag atacttgatg
    57061 tcgcccgagc cgtcctcgcg acgggacaag gtgcgcccat cgttcagggg gaagtgctca
    57121 ccatcgagcg ggttgcagtt ccagtccgga ggcagcccca gcggcgcctg atccagcgca
    57181 agcacccgcc aatggccgga catcacctcg tcagccaccc ccatgaagtc accctcggcc
    57241 gacacctccg gtaggagcca gcgcgcgccc gtccgatcca gcctgaacgg cggcaccggc
    57301 accgcgcccc ggtaccggcc cacgacctgg gcagccgtac tgcccgcacg cccgacaatg
    57361 tcggacaccg acatgtcgcg caggcgcttc cagtaccacc ctacccggtt cttgtcagcc
    57421 atctgttctc gttctcctgc tctgttgggg gattgggcgg atgccccagg cgttcaggga
    57481 cgctccgcca cacaatccgc cgtgctcgtc gagccgctcg gcccgcgccg gacgggcgtg
    57541 atccgaccgg acaccctcga cgttcggctt tgcacggatg ttcagtcaga tgccggatag
    57601 cgttcgcaaa ttaatacgca tttatctcgt atttcccaac ttcatgggat ttcaccggaa
    57661 ttaattcaca attttacaat gtttaatgtt ttgtgaaaaa ttggtgaacg tgacccagtt
    57721 ggcaagatgg cgatcgcctt gcacatgcgg cgacccgtcc gtacccgatt ctcggggagg
    57781 ggtcgccact gcaaaacgtg acgagacgac gcctcagcac agcggctgac gcgctaacgg
    57841 aacgggcgtg gggtcgtggc aggcgcttcg ctggcggaac gggcagcacg gcccgctctg
    57901 ccagaattca ccggcggaag cgattgatgt cgtcgcgggt ggcaagcgcc acctggcggg
    57961 ccgcagcagc gaaatcctca ccggagccgg catagaggat ggcgcgcgag gaggagatca
    58021 tcaggccggc ccccaaaggc gtccggccag ccgtcaccgt ggcctccaca tcgcccccct
    58081 gggcgccgat gccgggcacc agcaaaggca catcgccgac aatctgacgc acgtcggcga
    58141 gttcctccgg gaacgtggcc cccaccacca gcccgcactg gccatggaga ttccactttt
    58201 ccgccaccag ctccgccacg tgctgataga gcaggcgccc attcgacagc gtgaggttct
    58261 gaaggtcgga tccgccgggg ttggaggtcc ggcacaacac gatcagcccc cgatcgggat
    58321 aggccagata cggctccacc gaatcgaaac ccatgtaggg attgaccgtg agcgcgtcag
    58381 cctgaaagcg ctcgaaagcc tcgcgggcat attgctgtgc ggtcgcgccg atgtcacccc
    58441 gcttcgcatc cagaatgacc ggtacctgcg gatggcgatc atggatgtgg gcgatgagcg
    58501 cctccagctg gtcttcggca cgcgccgctg cgaaataggc aatctgcggc ttgaacgcac
    58561 aggcgacatc cgccgtggcg tccacgattt ccagacagaa gcgcaggact gcgtccggac
    58621 gacctttgag gtgcgcggga aagcgtgccg gatcgggatc aagccccacg cacaacaggg
    58681 aatcttggct gtgccaggca gcagcgagac gggaacggaa atccatcgaa gacacaggga
    58741 tcggaccaag agtatggaag gcgagattct accagccgcg gccggcgcaa gcccgcgctc
    58801 ggcggcggag ccacatcggt gggcgccacg tcaaacgccg ctgtcattga tccggtgccc
    58861 gccccgactc atcgccgtcg gcctcggcaa tgcacgcgaa gggatcgctc atgctgcgtt
    58921 acagcatgct ccattccgtg tcatgaactg cttcacgctt ttcgtcgctc catggggcac
    58981 tgttcaattt gtgcacacag cctcctgtcg gcccgaaccc cgagcgcccg cctcccaaag
    59041 cagaacattc ctttacaaac aatcggttgc aatttacaga caaaaagtcg ggacaggttc
    59101 ccgtacgacc tggcatgccc ctcgcgatag taaatgtgct ccggcgtgtt tcccgaaaac
    59161 acgaccctgg gaggaacgaa gtagcggccg ctcttgcccc gcgctttgct ggtgacacca
    59221 aaaaatctat cggagacatc tcatatgaaa aagtccattc tggctcaatc cgttgccctc
    59281 gcctgcctgg ccgctgccgc cggcggcgct caagccgtca gcttcacgca gaacgatgtg
    59341 accgtcgata tcaacggcac catcaacggc ttctacgtga accgggaatc caaggtcacc
    59401 aacctgagca ccggcgtcca gaccaagacc cagaacagtg aactgaccaa cggcctgctg
    59461 cccggctgga tcaacttcgt cgtcacctcc aaggccggcg atcaggacat caaggcccac
    59521 ttcagcttcg cgcccggcat caacaacaac tccaccgtcg taggtctgcc catcggcaat
    59581 aacgccagcg gcgcgggcgt gaccaacccg tacagccaga tcgacacccg taacgtctat
    59641 ttccaattcg gcaacaacga atggggcagc ctgaagttcg gccgtgacat cggcctgttc
    59701 ggtcagaaca tcatcctgtc cgacatgacc ctgctgggcg tcggcggcac ctccaacgcc
    59761 ggcatcccgt acaacaccac cttcggcatg atcggccacg gttacatgta caccggcttc
    59821 caggcccaga tcacctactc caccccgaac tacaacggcc tgcaggcctc cgccggcatc
    59881 ttccagccga gcaagttcgc cggtgacgaa accaaggcgc ccggcttcca ggccaaggtg
    59941 gattacgact ggaagggcgc catgcccggt aaggtgtggg gcggcctcgt gtcccagaac
    60001 accagctgct cgaatgcttc caccaccgcc tgcgccggca tgaaaccgta taacgcgacc
    60061 ggttatgaag ccggcgccaa ggcgggttac aacaacttcg aagcagtcgc ctacggcttc
    60121 accggcaagg gcctgggcct gtccaccatc ggcgcccaat tcctcggcgg cagcgacggc
    60181 aacggcaaca agaccgattc caagggctac ttcctgcaag gcacctacaa gatggacaag
    60241 accaagttcg gcattaacta cggccagaac aaggacaccg gtggcggcac tcaaagtgtg
    60301 tacggcgcgc ccgaaaccaa gaacaaggct tacaccctgg gtgtgtacca cggcctgaac
    60361 aagttcgtga ccctcgtcgg cgaattcaac aacgagaaga tcaccaacac ggccacggaa
    60421 accaagaaca acaccatttc cgcgggtgcc atcctgttct tctaaggatc tttcgccagt
    60481 cgaaagttgc aagaccctgt tgtgtttctc cctccttccg gcgcccctcg gggcgccgtt
    60541 tttttgtcca c
//
LOCUS       DAOQMM010000009        59875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_86_length_59875_cov_52.486543, whole genome shotgun sequence.
ACCESSION   DAOQMM010000009 DAOQMM010000000
VERSION     DAOQMM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 59875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59875
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_59875_cov_52.486543"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1304)
                     /gene="icd"
                     /locus_tag="PLH85_02770"
     CDS             complement(60..1304)
                     /gene="icd"
                     /locus_tag="PLH85_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNB63508.1"
                     /translation="MSTSLIKVPAGGQKIVPGQPTPDNPIIPYIEGDGIGVDITPVMI
                     QVIDAAVTKAYGGQRKIHWMEVYAGEKSTQLYGPDEWFPKETFDALKEFSVSIKGPMT
                     TPVGGGIRSLNVALRQELDLYQCVRPVRYFKGVPSPLKQPELTDMVIFRENTEDIYAG
                     IEWAAESDNVKKVIAFLQQEMGVKKIRFPESSGIGIKPVSIEGTTRLVRAAINYAIAN
                     DRKSVTLVHKGNIMKFTEGLFRDTGYKVAQQEFGAQPIDGGPWCSFVNPRTGRTIIVK
                     DAIADAFLQQILLRPAEYDVIATTNLNGDYISDALAAQVGGIGIAPGANISDQYACFE
                     ATHGTAPKYAGLDKVNPGSLILSAEMMLRHLGWVEAADLVIKAMEAAIGDKQVTYDFA
                     RLMEGATEVSCSGFGEAMVARM"
     gene            1640..3412
                     /gene="aceK"
                     /locus_tag="PLH85_02775"
     CDS             1640..3412
                     /gene="aceK"
                     /locus_tag="PLH85_02775"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNB63509.1"
                     /translation="MDTHGNENPVAQAIAQAMIEGFNKHYRIFRETSRHAKESFEAAD
                     WQDQLSAVRNRVQFYDDRVNETVQRLHEEFDAESIDDATWQAVKLHYIGLLLNHKQPE
                     LAETFFNSVTTKILHRDYFSNDYIFTRPAISTDYIESFPPTYSSYYPQDEGLRATVRR
                     IVEDFDWQRSFEDLERDVDYVLRTVETHLGGWPRMEVNCQIQVLYSAFYRNKTAYIIG
                     KGLNGYQEIPFALAVRHGASGMLYLDTILLDTWRISLLFSLSRAYFLVDMEVPSGYVQ
                     FLRSIMPNKPRSELYTMLGLGKQGKTSFFRDLVSHLRHSNDQFIEAPGIRGLVMLVFT
                     LPSYPYVFKIIKDTFGSSKNMDRATVKRKYLLVRQVDRVGRMADTLEFSNVALPRSRF
                     HPDLILQLRTLAPSVIEEDGDTLVVKHLYIERRMTPLNLYLDKASDEQVEEAVKEYGQ
                     AISELAIANIFPGDMLWKNFGVTRYGRVVFYDYDEIEYMTDCNFRRIPPAPNEDMEMS
                     GEPWYSAGPHDIFPEEFATFLLGQPKIRKAFLKHHRALLDPRFWQEAQEKIRNGYVED
                     FYPYPQALRFRTLYAERPDEPPPA"
     gene            3569..6241
                     /locus_tag="PLH85_02780"
     CDS             3569..6241
                     /locus_tag="PLH85_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HNB63510.1"
                     /translation="MQNSFQIRALAAAIGSALVLAACGGGGSTGYSQINGTAAEGTAI
                     ANSTLTVKDASGKTRSVTTDASGNYTLSLADLSFPFMLQITGSQGTWHALVSSDDVGK
                     PVNVNNATDSIAQLALGVSGNAALRAAFTAGSFKSVTGQAIASADSKLLDALEAELGK
                     RPESLRSATFTPATDSTPGDETDRLLTLVDAQPSGSGFATFNRVPEFVWTDSIVTKTY
                     DGTTDDLLTAGLGKTGLTSATAPAYVNAASPTAAELRRNAIHTNYRALVDANKTAGGW
                     TTLYGPNIDTSGNDTLGEGRIAGDETIAYSGDSSGKRKVTLMVQVPASFDKTKPCIVT
                     ATSSGSRGIYGAIGTAGDWGLKHGCAVAYTDKGSGNGMHDLAADTVNLIDGTASSSAS
                     AGKRALFAANLSKAELDAYNLKSPNRIAYKHAHSQQNPEKDWGRNTLDAVGFAFYVLN
                     EKYGTVSNGKKLRDLTPRNTLVIASSASNGAGAALQAAELDKLGLIDGVAVTEPQIQP
                     KDVSSLTIKQGSNTVATIGKPLIDYFTYANIYQPCAALATAASGSPGASLVAFYATNR
                     CTSLKEKGLVSGTTTQEQADDALQKLHAYGWAAEQDVLHASHHALATPSIVVTYINTY
                     GRFNVTDEVCGFTFATTVASGATLGNVTATSALVQAGIFATGNGVPPTAGINLVYNDS
                     VGGAKRDVLAISPSTNRADGALDGALCARSLVTGSDPVTGAKLTGTLLAQSQRVRQGI
                     DEVLFKGELGGKPAIIVSGRSDTLIPVNHASRAYYGMSQKADGTTSRLRYYEVTNAQH
                     FDAFIDNNAAVAGYDTRLVPLHVYFNRAMDLMYAYLKTGAALPASQVIRTTPRGGTAG
                     AAPAISATNVPAIATTPAAGDTITYSSGTLSIPD"
     gene            complement(6321..6749)
                     /locus_tag="PLH85_02785"
     CDS             complement(6321..6749)
                     /locus_tag="PLH85_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB63511.1"
                     /translation="MSRRGIPTGVHVLFERDSRVLLMRRAGTGFFDGLYSLPGGHVEE
                     GESVGMTAHREMAEELGVTLAPDAVALLGVVHRRSDTNRIDFFVRALTWSGEPRIAEP
                     DKCDDLAWFGRDALPLALVPYVRDALAAGDGPWLMESGWD"
     gene            complement(6751..7200)
                     /gene="dut"
                     /locus_tag="PLH85_02790"
     CDS             complement(6751..7200)
                     /gene="dut"
                     /locus_tag="PLH85_02790"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNB63512.1"
                     /translation="MHSIDIRILDGRLKEQPPAYATSGAAGLDLRACLEAPAHLLPGA
                     TLLVPTGIAIHLADPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSVWNRG
                     HDSFTIQPMERIAQLVVVPVLQVAFNLVESFDVSERGAGGFGSTGKH"
     gene            complement(7225..8418)
                     /gene="coaBC"
                     /locus_tag="PLH85_02795"
     CDS             complement(7225..8418)
                     /gene="coaBC"
                     /locus_tag="PLH85_02795"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNB63513.1"
                     /translation="MAELSGRKIVLGVTGGVAAYKAAELVRLLGKAGAEVHVVLTEGG
                     ARFVTAVTFQALSGRPVWTDLWDPRMANNMAHIDLSRDADAILIAPATADVMARLAQG
                     RADDLLTTLCLARDCPLLVAPAMNRQMWEHPATRRNAVQLAADGVTLFGPAAGEQACG
                     EVGLGRMLEPEDLCEALIGFFQSKLLAGRRVVMTAGPTFEAIDPVRGITNSSSGKMAY
                     ALARACAQAGADVALVSGPVALPVPAGVARISVRSALQMRDAVFAALPGADVFIGVAA
                     VADYRPDTSAEHKIKKSGAALQISLTPNPDILAEVAARADAPFCVGFAAESRNLDEYA
                     EGKRRAKKLPLVVGNLVQDGLGSDDNQVVLYDEAGRHPLERAPKPEVARAIVAHLAGL
                     LPPLS"
     gene            8465..9142
                     /gene="radC"
                     /locus_tag="PLH85_02800"
     CDS             8465..9142
                     /gene="radC"
                     /locus_tag="PLH85_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNB63514.1"
                     /translation="MAITDWPADERPRERLLAHGAEVLSDAELLALFLRVGVRGKSAV
                     DLARDLLGRFGSLSALCTASVDDFSTIPGMGPAKYAQLQAVLELARRALSEEMASRDV
                     LDSPRAVRDWLRLKLAGLPHEVFMVLLLDAQNRVIASEELFRGTLAQTSVYPREVVKL
                     ALLRNAAAVILAHNHPSGVGEPSHADQLLTRSLTQALALIDVRVLDHFIVAGTASPVS
                     FAERGLL"
     gene            9239..9475
                     /gene="rpmB"
                     /locus_tag="PLH85_02805"
     CDS             9239..9475
                     /gene="rpmB"
                     /locus_tag="PLH85_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNB63515.1"
                     /translation="MARVCQVTGKAPMVGNNVSHANNKTKRRFLPNLQNRKFWSEAEN
                     RWVRLRVTNAALRTIDKKGIDTVIAELRARGEKV"
     gene            9504..9671
                     /gene="rpmG"
                     /locus_tag="PLH85_02810"
     CDS             9504..9671
                     /gene="rpmG"
                     /locus_tag="PLH85_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNB63516.1"
                     /translation="MAKGGREKIKLESTAGTGHFYTTTKNKRTTPNKLEFSKYDPVAR
                     KHVPYKEVKLK"
     gene            complement(9922..10125)
                     /locus_tag="PLH85_02815"
     CDS             complement(9922..10125)
                     /locus_tag="PLH85_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB63517.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGSEDLFAHFSAISMSGFKSLKE
                     GEKVQFEVTQGPKGKQASNIQRA"
     gene            10536..10844
                     /gene="clpS"
                     /locus_tag="PLH85_02820"
     CDS             10536..10844
                     /gene="clpS"
                     /locus_tag="PLH85_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNB63518.1"
                     /translation="MVTKKQGESVLETEVTRIKPPPLFKVMLLNDDFTPMDFVVTVIE
                     KFFGLDREQATRIMLKVHREGVGVCGIFPRDIAATKVEQVIAYARQNQHPLACVMEEN
                     "
     gene            10846..13125
                     /gene="clpA"
                     /locus_tag="PLH85_02825"
     CDS             10846..13125
                     /gene="clpA"
                     /locus_tag="PLH85_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNB63519.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPSAAEVL
                     RACAARSDELRKELTNFINEHTPRVEGTEDIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLQRQNISRLDVVNFISHGITKAPQSGASPARPNESSEQ
                     GEAEPENPSAGGALDSFTQNLNQQALLGKIDPLIGREKEVERVIQTLCRRRKNNPLLV
                     GEAGVGKTAIAEGLARRIVEGRVPEILADAQVYALDMGALLAGTKYRGDFEQRLKAVL
                     KQLVDHNNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGQLKCIGATTYTEFRQ
                     IFEKDHALSRRFQKVDVVEPSVPETVEILKGLKSRFEQHHGIKYSASALSSAAELSAR
                     YINDRHLPDKAIDVIDEAGAAQRILPKSKQKKLIGKTEIEEIVAKIARIPPRNVSNDD
                     KAALRTLERDLKNVVFGQDAAIEALAKAIKMSRSGLGNPQKPIGSFLFSGPTGVGKTE
                     VARQLAYTLGIELVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEQITKKPHCVL
                     LLDEIEKAHPDIYNILLQVMDHGTLTDNNGRQADFRNAIIIMTTNAGAETMQKSVIGF
                     SAKREVGDEMAEIKRMFSPEFRNRLDATISFKSLDHDIVMKVVDKFLMQLEAQLHEKK
                     VDAHFTDRLKEWLGQKGFDPLMGARPMARLIQDTLRASLADELLFGRLAGGGHVTIDL
                     DDNGKVALQFEEEADVATV"
     gene            13206..13685
                     /gene="rraA"
                     /locus_tag="PLH85_02830"
     CDS             13206..13685
                     /gene="rraA"
                     /locus_tag="PLH85_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HNB63520.1"
                     /translation="MTFQTTDLCDANEGKVRVVAPMFRSFGGKTRFAGPISTLKVFED
                     NSLVRSALETAGAGRVLVVDGGGSMRCAMVGDQLAALGVKNGWAGIVVYGCIRDSGPI
                     AGMDIGVFALGTHPQKSVKKGAGDQDIPVTFGGVTFVPGHWLYADEDGVIVADAPLV"
     gene            13988..15301
                     /gene="aceA"
                     /locus_tag="PLH85_02835"
     CDS             13988..15301
                     /gene="aceA"
                     /locus_tag="PLH85_02835"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNB63521.1"
                     /translation="MSLTREQQIAALEKDWAENPRWKLVKRGYSAADVVRLRGSLQPE
                     YTLAQRGAQVLWDKVNGAAKKGYVNAFGAITAGQAMQQAKAGLEAVYLSGWQVAADGN
                     TSETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWSRGVNPGDEGFIDYFLPIVADA
                     EAGFGGVLNAFELMKNMIAAGAAGVHFEDQLAAAKKCGHMGGKVLVPTREAIEKLISA
                     RFAADVMGVPTLILARTDAEAANLITSDYDENDKPFLTGERTQEGFFRVKNGLEQSIS
                     RGVAYAPYADLVWCETGVPDIGFAREFAQAIQAACPGKLLSYNCSPSFNWKKNLNDSQ
                     IASFQEELSALGYKYQFITLAGIHVNWYNTFQFAHAYARGEGMKHYVEMVQEPEFAAR
                     EKGYTFVSHQQEVGTGYFDDVTTVIQGGSSSVKALTGSTEEEQFH"
     gene            15466..16329
                     /locus_tag="PLH85_02840"
     CDS             15466..16329
                     /locus_tag="PLH85_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB63522.1"
                     /translation="MQSLWMIAASFLFACMGVCVKLGALRFSTGELVFYRGLIGLLMM
                     AVLLRVQGIPFATPHWKLQLSRGLSGTVALMCYFFALGILPLATAVTLNYTSPIFVAL
                     ILALWFREPVGPLRHAAVALGFVGVVLLLKPTLQPDQWPGALGGLGSGLIASLAYINV
                     RELGRAGEPEARTVLWFSLITAAFGLPWALGSDHLHRLDASGLAIVLGVGGFGGCAQL
                     AMTRAYRYGKTIVSANLAYTTVIFSSAFGALLWDERLPWTSFVAIALIVASGVLVSLA
                     SRKPAPVAESD"
     gene            16402..16764
                     /locus_tag="PLH85_02845"
     CDS             16402..16764
                     /locus_tag="PLH85_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNB63523.1"
                     /translation="MITITHDAGQVDAVVFGEFTLSDYKEFEEVFNYTVRFEGPVNLF
                     FDLRAMSGFTVDMALEEIRFARAHANDFAQIAVVTSDQWVSWSAWLSQLFVNADVRVF
                     DDADDARAWLAGVESPVQ"
     gene            16777..17631
                     /locus_tag="PLH85_02850"
     CDS             16777..17631
                     /locus_tag="PLH85_02850"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNB63524.1"
                     /translation="MNGTFGTLISAAELADQADANWRLFDCRFDLRNRRYGRDAYASG
                     HLPGAVYLDLEEDLSGPLSGLNGRHPLPDPQLLAAKLGSLGVTTGTQVVAYDDAGGMF
                     AARLWWLLRWLGHPTVALLDGGIQAWASSGRALSQDVPALTARVFSPRLQPALHVDAT
                     YVHGHLDDPALCLIDARAPDRFRGENETLDPVGGHIPGAINRFFRDNLGPDGLFKPAA
                     QLQEEFGTLIKGGDAHRVIHQCGSGVTACHNILAMEVAGLTDSRLYAGSWSEWCADPA
                     RPVATGPG"
     gene            complement(17658..18086)
                     /locus_tag="PLH85_02855"
     CDS             complement(17658..18086)
                     /locus_tag="PLH85_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNB63525.1"
                     /translation="MGLLDQLAGQVLGSLGGGQGQGGSDLQHMLLQAVLGLIQNGQGG
                     LGGLVAQLNQAGLGEQVASWVGTGQNLPVSADQISQALGGRLGDMAAQLGLPADQVAG
                     HVAEMLPQVVDHLTPDGQMPQGGDLLSAGLSALGGLFGKA"
     gene            18265..18927
                     /gene="rpiA"
                     /locus_tag="PLH85_02860"
     CDS             18265..18927
                     /gene="rpiA"
                     /locus_tag="PLH85_02860"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNB63526.1"
                     /translation="MTQDELKQLAALKALDYVQDGTIVGVGTGSTANFFIDGLAARKE
                     RIVGAVPSSEASAKRLAAHGIRLLDLNDVADIPVYIDGADEIDGTLAMIKGGGGAQTR
                     EKIVAAVAQRFVCICDASKKVAQLGRFPLPVEVIPMARAQVSRELERLGGRPVLREGF
                     VTDNGNLILDVHGLNIHDPVTLEIAIDGIVGVVTSGLFAKRGADVLLLATAQGVQTFE
                     RA"
     gene            18991..19689
                     /gene="phoU"
                     /locus_tag="PLH85_02865"
     CDS             18991..19689
                     /gene="phoU"
                     /locus_tag="PLH85_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNB63527.1"
                     /translation="MNEHTSKKFDAELEGIRTRVLQMGGMVELQIVKAMEGLAHGDLL
                     LIDQTIENDARVNSLEVELDEFCSQIIAKRQPAAVDLRMISTVQKTITDLERIGDEAK
                     KIAKIAKQIHASASPFMPQIRLGHIADAVVDMLRTALDAYARLDTVAAAQVVRQDKDV
                     DAEFKGIMRQLITYMIEDPRTISHAIDMMFVAKSVERIGDHAKNIAEYVVYMVKGRDV
                     RHTSLEEVEREATR"
     gene            19836..20600
                     /locus_tag="PLH85_02870"
     CDS             19836..20600
                     /locus_tag="PLH85_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNB63528.1"
                     /translation="MPLKFELLQADDYSQYLLREKTDILFNLRGLLQKRAMISAFIDA
                     SADSFLTALLAVTPDERHLILDAAADDSLNRRVEGAEQLICVAQLEKIKIQFAAREIV
                     RFPFEGHEAFRVRMPDVVLRLQRREYYRLTAPISHSLSCLIPVAEGESARPRSVEASV
                     IDISGGGIAVLVPPQGLAFEPEMEFADCRLMLPETGPIVTAIKVRNLFRITHPNGQVM
                     LRAGCEFLGLPGNMSTTIQRYILRTERELNARHRIR"
     gene            complement(20671..20991)
                     /gene="fliE"
                     /locus_tag="PLH85_02875"
     CDS             complement(20671..20991)
                     /gene="fliE"
                     /locus_tag="PLH85_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNB63529.1"
                     /translation="MDTRGVDQMLSELRATAQTAAGKPAQAAAATGQADFGQVLQSAL
                     DQVSSAQMQAQEMAKGFSSEDPNVNLQDVMVNLQKANLSFQQMVQVRNKLVTAYQDIM
                     NMSV"
     gene            complement(21054..22436)
                     /locus_tag="PLH85_02880"
     CDS             complement(21054..22436)
                     /locus_tag="PLH85_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB63530.1"
                     /translation="MNDALHILVVEDDAPLRDAVCLTLEVAGHTVTGVGDGPAALART
                     DDEAFNLVISDLKMQPMDGLELLAELRRRLPQLPVLLMTAFGDVEKAVDAMRGGACDF
                     LMKPFEPQVLLEHVRRYAAPDRDPGGMVAADPHTRKLLALAAKVAETDATVLLTGESG
                     TGKEVFARYLHEQSPRRNGPFVAINCAAIPDTLLEATLFGHEKGAFTGAATAQAGKFE
                     QAAGGTLLLDEISEMPLGLQAKLLRVLQEREVERVGGKKAIALDIRVLATTNRDMTKE
                     VAAGRFREDLYYRLNVFPLAIPSLRERPGDIVPLAHHFARLHGARLRRSARLSPEAES
                     VLVSYHWPGNVRELENTVQRALILSSGESVSGETVRLCLPHWNGEPLRPVLRPATPVP
                     ADFAGEALPGGKELPRPANMKDLEREHILQVLREVGGSRKRAVERLGISERTLRYKLQ
                     QYREEGFFPG"
     gene            complement(22475..23578)
                     /locus_tag="PLH85_02885"
     CDS             complement(22475..23578)
                     /locus_tag="PLH85_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB63531.1"
                     /translation="MIPTVAEDRLDARQLAEAFRQFSEASEALTGAYAGLQAQVGQLT
                     ERFSVLMEALPAGVVVLDRAGAVLQVNRVVERMFATVPPGTLWSHFSGSRLVATETPG
                     EWAVACGDGPRRVTLQEAPLESGEGGIVLINDVTEAHRARLAAERTGRLAAMGEMVAG
                     LAHQLRTPLSAALLYTATLAQQDLKPGDRDKIGLRALERLRHLEKLIRDMLLFARGET
                     LGREPFPICDLVAELAHTLEPVARAKGIALAVECDCGRTEVVGDRKAVAGALTNLLEN
                     AVQVLETGGHIRFDAGVEGAHACFRVADDGRGIAPELQSRLFEPFFTTRPDGTGLGLA
                     IARGVARAHGGDIGIASEPGRGTTFTLTLAMGA"
     gene            23734..25440
                     /gene="fliF"
                     /locus_tag="PLH85_02890"
     CDS             23734..25440
                     /gene="fliF"
                     /locus_tag="PLH85_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNB63532.1"
                     /translation="MAAEDAAAPPVPPPSPFTQVLENIRALSQRQKIAAGAALAAAIA
                     LVVGVLLWSRQPDYAVLFSNLAEKDGGAVVASLQQQNIPYKFSDNGNAILVPSTQVHD
                     LRLRLAAQGIPKGGLVGFELMETQKLGLSQFHEQVNFQRALEGELSRTISSIAAVAGA
                     RVHLAIPKQTAFLRDEQKPTASVMVNLHQSRSLDPSQVAGIVHLVASSVPNLTNDNVS
                     VIDQDGNLLTKKPDPMRAGLDATQIKYTRELEEGYIQRINDILTPLFGKGNFRAQVTA
                     DVDFNVTEQTAETYRPNPSPEQAIRSQQSSESQTRDAGAQGVPGALTNQPPVPATAPI
                     TTPAVPGTPGANGQLPLSTSKNATINYELDKTIQHTRKALGQIRRLSVAVALNQKEEK
                     DKNGNAKPVPLTEEERKRVEDLVRDAVGYSKDRGDTLSVASSPFVASEKDLPETPLWK
                     DPDNIALFKELLKYLVIAGVIAFVAFGVIRPLMKQVMPPPPEKAEEEASAESAGEAGE
                     EGGGEPGEEGEEGAEVELSPEAAARLAYEEKLARVRDLARSNPRVVANLIKEWMGVNE
                     QR"
     gene            25427..26425
                     /gene="fliG"
                     /locus_tag="PLH85_02895"
     CDS             25427..26425
                     /gene="fliG"
                     /locus_tag="PLH85_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNB63533.1"
                     /translation="MSSDEGLEKSALLLMTLGADEAAEVLKLLGPREVQKLGAAMAAM
                     PAQSRERMEAILDEVEALAEKGSPIEADHEHIKAMLTKALGDERASHLISRVLQGSDT
                     AGIESLKWMDAPTAADLIKNEHPQIIATILVHLEFDQAGEILKHFTDRLRNDVLLRIA
                     TLDGVQPVALRELNEALTRMLAGASSVKKTAMGGVRHAADILNFVGSAAETAILDNVR
                     EYDPDLAQKILDEMFVFENLLDLDDRGIQTLLREVQSDSLVIALKGAPPEMREKVFKN
                     MSQRAAEMLKEDLESRGPVRLSEVEAEQKEILKTARRLAEEGQIALSSKGGDDAFV"
     gene            26441..27172
                     /locus_tag="PLH85_02900"
     CDS             26441..27172
                     /locus_tag="PLH85_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNB63534.1"
                     /translation="MALNRHQASGAYRRWDFPSFDAPPPEALAPPPGEPPPDAAVTAT
                     APAPDPSEPPPVEASPAEAALPLPEPDIQLPTAEDIERIHEEARKEGYAAGYEEATAR
                     GRMEAMRLHGLVEGLEAAMSRLDADVAEEVLALSLEVARLMVRQTLATRPESVVDLIR
                     EALVQLPQAHAQIHLNPEDLGMVRDYLGELLTHAGHRLVEDAGVARGGARIEGGGSQV
                     DATVPTRWRRIMDNLGRNIPWEGEG"
     gene            27172..28575
                     /gene="fliI"
                     /locus_tag="PLH85_02905"
     CDS             27172..28575
                     /gene="fliI"
                     /locus_tag="PLH85_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNB63535.1"
                     /translation="MDHGLETWRAFLHDSRKLVNATSPLQLSGRLTRINGLVMEASGL
                     KLPLGSGCRIVVPGGGTVEAEVVGFHGEKIFLMPTDDVFGLAPGAQVLPVEPTQPQLI
                     AGERIEPRRRASDRAKHLPMGDALLGRVVDGAGRPLDGLGPLDVKHTRSLQARPVNPL
                     QRAPISRSLDVGIRAINALLTVGRGQRMGLFAGSGVGKSVLIGMMARYTAAEVIVVGL
                     IGERGREVKEFIEHNLGPEGLARSVVIAAPADTSPLMRLQGASYATTVAEYFRDQGKQ
                     VLLIMDSLTRYAMAQREIALAIGEPPVTRGYPPSVFARLPALVERAGNGPEGGGSITA
                     FYTVLAEGDDQQDPIADSARAILDGHIVLSRNLADQGHYPAIDIEQSISRAMHNLVKP
                     AHFDVVRRFKQLFSRYQRSRDLIAVGAYQAGSDPILDQAVAYYPRLEAFLQQRIEESE
                     PYESAVEKLEALFAGTS"
     gene            28642..29103
                     /gene="fliJ"
                     /locus_tag="PLH85_02910"
     CDS             28642..29103
                     /gene="fliJ"
                     /locus_tag="PLH85_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNB63536.1"
                     /translation="MTEPFPLKPLLDLANTRMDEAARRLGELIASEHAVEEKLALLKD
                     YRDEYQARFMEAARNGIGPDAWRNYSAFIARLDDAIAQQQKLASDSRRRTEQGQQAWR
                     DERNKVKAFDTLSQRHHTAQLRKELKQEQRLTDEHAAKHFRDRDADDDGEP"
     gene            29177..30298
                     /locus_tag="PLH85_02915"
     CDS             29177..30298
                     /locus_tag="PLH85_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNB63537.1"
                     /translation="MPATSLNPIATVAPPAPSGNASASRSGDARTPEASASADGDFGS
                     ALKQQVDRQADDRPRSADEAGSAGSKTRDDTAAAQAPAAPPGEAPPSAEAPQRLGLAL
                     FLGQATGRSEADGRPAARTDDDAPATESPSAALAGLLAPPIDPRPTTPAESAPADTTG
                     KSPAKTAAGLPEFAAAFRTTPTDAGSADAPRLPAGTDAAPANSFAGLHAAALAQIRGE
                     APTPASNALSLPVHTPATHAGWPEEVSNRVSWMVGQHESQAELTLTPPQLGKVEVTVS
                     VSGDQTSAQFVAATPAARDLIEQALPRLRELLGDAGIALGQTSVGTSGQSGNGEGGDG
                     PRRGTAGRGTRGVDGIGSVQGGSAGPWLRRGEGLVDTFA"
     gene            30391..30960
                     /locus_tag="PLH85_02920"
     CDS             30391..30960
                     /locus_tag="PLH85_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNB63538.1"
                     /translation="MSKAPAKAEAAEGEAPAKGGGKKKLIIIVAVVLILAIAGGAAFF
                     LLGKKKHAEGEEEEEPAHHEPPKVKVDPSKPPVFVQLDQFTVNLAPEEGEHFLQATMV
                     LRVADAKVGDSLKAYMPELKHRILMLLSSKRPSELANAEGREELADEVKDEANDVLGY
                     APNPKKKAKRRPEDDGPVLAVLFNQFIVQ"
     gene            30973..31974
                     /gene="fliM"
                     /locus_tag="PLH85_02925"
     CDS             30973..31974
                     /gene="fliM"
                     /locus_tag="PLH85_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNB63539.1"
                     /translation="MSQDFLSQEEVDALLKGVTGETDEPDSGEESQDGVRDYNLATQE
                     RIVRGRMPTMELINERFARYLRIGLFNYMHRNVEISVGPIKVQKFSEFVRNLVVPTNL
                     NLFIAKPLRGNALVVLDPNLVFLVVDNLFGGDGRFHTRVEGRDFTPTEQRIIQGLLKV
                     TFNEYEKAWSPVQKLDFEYVRSEMNPQFANIATPSEIVVAASFTLEFGGSQAEMHFCF
                     PYSMLEPIRETLYSTMQSDHITQDNRWVGMMGRQLQTAEVELVARLGTAHVTLRDIVG
                     MKPGDVIPLNIPETIQAEVDGIPIFECRYGIQRSQYALKVERFLAQDEDSPPAKAQE"
     gene            31978..32436
                     /gene="fliN"
                     /locus_tag="PLH85_02930"
     CDS             31978..32436
                     /gene="fliN"
                     /locus_tag="PLH85_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNB63540.1"
                     /translation="MSDTETDAPDDNQVSDDDWAAAMQEQAASEGLTDPATGVDESPY
                     QAKPASQLFPDFGQAGTKSGTLNDFDMILDIPVQLTVELGRTKIAIRNLLQLAHGSVV
                     ELDGLAGEPMDVLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERIRKLRS"
     gene            complement(32506..32712)
                     /locus_tag="PLH85_02935"
     CDS             complement(32506..32712)
                     /locus_tag="PLH85_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63541.1"
                     /translation="MLLWRREVQPVVKPVPEAVPVSAQADYEEKRQRALQILGERWLL
                     HRANAPDRRPVRSVLGGAAGRPLS"
     gene            32878..33285
                     /gene="fliO"
                     /locus_tag="PLH85_02940"
     CDS             32878..33285
                     /gene="fliO"
                     /locus_tag="PLH85_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNB63542.1"
                     /translation="MRRALVPAVLLSIGPVALAGDAAPDLTGSVVQTLLALGAVIGLL
                     FGTLHLLRRSGVARAGGAGLLQVRAATAVGPRERVVLVEVAGKLLVLGVAPGRVTPLH
                     TLDATDLPAVPSRPAGERDFQSWLRQTLERRAP"
     gene            33282..34022
                     /gene="fliP"
                     /locus_tag="PLH85_02945"
     CDS             33282..34022
                     /gene="fliP"
                     /locus_tag="PLH85_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264540.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNB63543.1"
                     /translation="MTAARLRRLTPLLLLLPLPALAQALPALTSGPAPGGGTQYSLPV
                     QTLLLLTALSFLPAMLLMMTSFTRIIIVMSLMRHALGTQTSPPNQVIVGLSLFLTFFI
                     MAPVGDRIYTDAWVPLQENRISFDQAVERASVPVKGFMLRQVREPDLNLFSKLANTPK
                     VDKAEDLPLRVVIPAFVTSELKTAFQIGFIIFIPFLIIDMVVASVLMSMGMMMMSPAI
                     VALPFKIMLFVLVDGWNLLVASLVQSFG"
     gene            34047..34316
                     /gene="fliQ"
                     /locus_tag="PLH85_02950"
     CDS             34047..34316
                     /gene="fliQ"
                     /locus_tag="PLH85_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNB63544.1"
                     /translation="MTPTTVIDLGRQALELTLLVSAPLFIAALATGLVVSIFQAATQI
                     NEQTLSFVPKLVATFITLVLAGPWMITLLTDFIRRLFESIPAMIG"
     gene            34319..35101
                     /gene="fliR"
                     /locus_tag="PLH85_02955"
     CDS             34319..35101
                     /gene="fliR"
                     /locus_tag="PLH85_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNB63545.1"
                     /translation="MLTVTAAQLDAWLALFAYPLARILAMVSSAPVFGNTALPRRIRL
                     VAGLALALGIVPALAPIPAIPLGSWSGLLTIFQQTLVGLAMGLAMRVVFAALDVLGEL
                     TSLQMGLSFATFFDPGSNGQTAVVGEFLTLLATLIFLALNGHLLMADVLARSFEWLPV
                     NALPLHAGGWAVLVRFGAVTFSAGLLLALPVVTALLITNIALGILTRAAPQLNLFAIG
                     FPITLTVGFGVIMLALERFAPLLQRFYDMGFDTIAALLKALA"
     gene            35119..38250
                     /locus_tag="PLH85_02960"
     CDS             35119..38250
                     /locus_tag="PLH85_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB63546.1"
                     /translation="MFAISPSAALALSLAISMLLAASLRYVAAVERPQTALRAWAAAF
                     ACHAVHDAALLAPALTHLPLALLAPVGLMGMSAALWIGLGRVTGDGHSPRVMLLPLLA
                     ALWALVPTAPGRSEVLRTLPVDAVAITFTFAAARVFWRRATYQPGTGHRLAAVGLGGW
                     GLVLLAGSAGVLADGSVARALAAALALSGLGCALLINALCLQNAAEARLTTHLRAEMD
                     RRIAAESALAESHSRFTALAESSDLGLFVVSPDGQFQYTNSRYRALSGLTDEELRTRP
                     DTQRMDGEDAARVSAAWAEARALRCQTHYERRVVQSGATEGWLSVHVAPITAGDSLQG
                     FVATVEDITARKQAEAALRDSEERLSQVFRLLPDAVMLSDPDSGRIDDVNQGWTDTLG
                     HPREATLGRTAEALGLWCNPSDARTVLDTLRAGHDVSDQPATLCHRDGRVLETLLRAR
                     LIRVRGRDGLLTVIRDVTEPRRLERIGRAAADALRVSEAMFSAAFRASPDYLTISRLD
                     GHLLEVNPTFERMTGWRRDEAIGAQAVELGLWAFPEEREAMLHELSVHGAVTDFPCTL
                     GTRWKTRRTCLLNVSMVTLENEPCLVGMIHDITEQQAAEKALRDSERKFATIFNLAPF
                     ALSLTRVGDRTHVDANPAWERLFGIERNTVLGRTSGEFGCWTDPRAYDDLYARVVSSD
                     VLAQQEIRIRPCNRDDVATCLISGQRLRIRDEECALWAMVDISELRRAQARIEELNQG
                     LELRVVERTTELSNALEALRRTQEDLVRSEKLAALGSMVAGIAHELNTPLGNSVTVTS
                     TLATRTDELTHRKAAGPLRRSDLESYLDSVRTASDLLLRSLSRARDLVSSFKQVAVDQ
                     TSEQRRRFELGEVLREVVTTLGPMFKGTPYTLRIEPAPALEMDSYPGPLGQVITNLVS
                     NALVHAFAGRDHGTITLTPAADGPAGVTLCFRDDGHGIDPAHLGRIFDPFFTTRLGTG
                     GSGLGLHIVYNMVTRVLGGRLEVSSQLGVGTTFTLAMPRTAPATGGTENPMSISAKPA
                     GRSFNFEDNGPII"
     gene            38297..39337
                     /locus_tag="PLH85_02965"
     CDS             38297..39337
                     /locus_tag="PLH85_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB63547.1"
                     /translation="MAIAIVDDTPLNLTLIQALVRKIAPPDTEIRCFTSPLEGLQWCS
                     EHEPDLVIVDYMMPDLNGIEFITCLRKVHPAEAVPILMVTAAHEKEIRYQSLTAGASD
                     FLTKPIDRYEFDPRVRTMLALRLSHLRMRHYNDDLREAVRRATADIVARERETVTRLA
                     RAAEFRDPETGAHILRMAHYSRLIAEQLGLGAEFCERLLSAAPMHDVGKVGTPDHILL
                     KPGRLTDEEMAIMRQHASIGHDILKGSSSPMIQLAAEIALSHHEKFDGSGYPGGLTGE
                     AIPLSGRIVAVADVFDALTSERPYKPAWALDRAVQFLKDGRGAHFDPACIDAFLLRWD
                     EVLAIRDRYRDE"
     gene            39334..42444
                     /locus_tag="PLH85_02970"
     CDS             39334..42444
                     /locus_tag="PLH85_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB63548.1"
                     /translation="MKALFDRLASLLGLDSLRQQLTLLFTVLLGLAIGIYALYTAVEQ
                     AAYVERLERQRAEESARHLADDLAPHLTAGDRTGLTHHLEALARQRDMLHLTVTDLHG
                     LPVAGVSVTEGAPRFDAAADRPLTLPADTQPATRQGETDAIVAWAEIRDDVPRGWLRL
                     EIAPTAAREALAHIVEDSLITGLLTVTLGAVIIRRFLRGPLHSLERATAFAEGLETGE
                     GAVLNEPSRLEEIRKLVDALNWTSIRFYDGQSALADSESRNRAITEAALDCIVTVDDG
                     GRIIEFNPAAERSFGVSRADALHQYVGDLIFSDERRDEQERILQQVLDPERRDEAGQR
                     FETLVTRAGGQTFPVDVAIVPAISSGRRLFTAYLRDISDQKRAEEAMRMAMEAAEANS
                     RLKSDFLANMSHEIRTPMNAILGMTDLALDTDLDAEQREYLTLVKSSAQSLLNIINDI
                     LDFSKIEAGKLDFEHIDFSLRDCISLATRTLQQRAAEKDLQLVTQIEPQTPDTLRGDP
                     HRLRQVLINLISNGIKFTPHGEVRIRVHPTPGNKDAGRIELTFSVQDTGIGIPRDKQG
                     LIFDAFSQADTSTTRRYGGTGLGLAISAQLVKGMGGGIRVTSEPGQGSTFEFNAVFEP
                     GQSVVPVETDTELEGLPVLVVVKSASDRGILSELLTHWRMSPLMVSDASAAHRELLAA
                     AKRGSPYRVALLGRDLPDMDGFDLASALSAASPRPLSVILLAERGRRGDGARCREMGI
                     AAYLPMPIQASDLLNAILLSCDVGGEADGDRPLITRHSLREQKRVLNILLAEDNPINQ
                     TLALRLLAKLGHQVTVANNGAEALELHARQRFDIILMDVQMPVMGGFETTAEIRRREA
                     AGTPHTPIVAMTAHAMKGDRERCLDAGMDGYLSKPINAPDLVEALIHHADQSDPPPQP
                     VAHETVALGPVFDRAAVLANLGDDEELLTQLIAMYLHDEPRMVGELENAIAHQDAEAV
                     NAAAHALKGAVANFFACRAQARAMQLEQLGRHKHLSDAPAVLAELKRELAALHEAFRS
                     VAPADA"
     gene            complement(42490..42882)
                     /gene="gloA"
                     /locus_tag="PLH85_02975"
     CDS             complement(42490..42882)
                     /gene="gloA"
                     /locus_tag="PLH85_02975"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNB63549.1"
                     /translation="MRILHTMLRVGDLERSIAFYTEVLGMRLLRRSDYPDGQFTLAFL
                     GFQDEADGAVIELTYNWGVTSYALGTAYGHIALEVPDAYAACAEIKARGGKVTREAGP
                     MKHGSTVIAFVEDPDGYKVELIQAKNRG"
     gene            complement(42897..43646)
                     /locus_tag="PLH85_02980"
     CDS             complement(42897..43646)
                     /locus_tag="PLH85_02980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNB63550.1"
                     /translation="MSLPASPRKDGLQREVLLDGVAIAYTLKRSARRTFGLRVDARGL
                     TVGVPLRATQRAVDAFLHDHARWILDKVQVHAARAEAPPFAVVEGAGLPVLGETWTLH
                     LSKGLNRLVWHEGGVTADAPRILEIALRAGSDPAAVLRRGVQQRALEVFRHRVAEFCL
                     RIGQPVPTVGLSSARTRWGSCSRRSGIRLHWRLIHLPLELVDYVAAHEVAHLLEMNHS
                     PRFWSVVESVYPEHVVARARLRAVAASLPQL"
     gene            complement(43643..44410)
                     /locus_tag="PLH85_02985"
     CDS             complement(43643..44410)
                     /locus_tag="PLH85_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB63551.1"
                     /translation="MVLIRNLLFMLALAVFTPLYALIGVLSSPFGPQTRHKLIRGWPR
                     IMGWVIKHVLGIPYRLVGAENIPREGPAIIVSKHMSAWETILLQDIFPPMVFVLKKEI
                     HSIPFFGWGIARMPMIAIDRAAGKDALTQVEAQGRERLAEGFWVTIFPEGTRVAPGSK
                     KRYKAGASWLAAQTGVPVVPVAHNAGEFWPRNALFKRPGVVTVSVGPVIDTKGLNAEQ
                     INAKAQAWIEGEMRQLFPGHYRPTRQRAAEAVEPAAE"
     gene            complement(44421..44954)
                     /gene="gmhB"
                     /locus_tag="PLH85_02990"
     CDS             complement(44421..44954)
                     /gene="gmhB"
                     /locus_tag="PLH85_02990"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNB63552.1"
                     /translation="MKIIVLDRDGVINQDSDQFIKSPEEWKPIPGSLEAIARLNESGW
                     RVVIASNQSGVGRGLFDMDTLNAINEKMVKALAQAGGRLDAIFFCPHAADSTCDCRKP
                     KPGMFRQIAERFNVDLRGQPVVGDSLRDLQAGVAVGCAPYLVLTGKGGKTRLDPDLPE
                     GTQIFPDLAAVVAQITA"
     gene            complement(44972..47056)
                     /gene="glyS"
                     /locus_tag="PLH85_02995"
     CDS             complement(44972..47056)
                     /gene="glyS"
                     /locus_tag="PLH85_02995"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNB63553.1"
                     /translation="MTGATLLVELLTEELPPKALPRLGQTFAKGVVEGLRARGLAAAE
                     GAFRWFATPRRLAVSVSGVVAEAAARDVTEKIMPVSVALDAAGQPTPALVKKLEAKGI
                     PLSAVATFERRLDGKAEALFHTATVPGARLADVLAGIVQDALKALPIPKVMRWGDSDV
                     AFVRPVHSLLMLHGADVVPGTVLELAAGRVTRGHRFMSRGEIVVATADAYEPTLLAEG
                     KVVPDFAERRADIEQQLVREAARQAASLGQYADLLDEVTALVEHPTVYVGEFEAEFLA
                     VPQECLILTMRANQKYFPLFDAQGKLLNRFLIVSNMRLEDPSNIVTGNQRVVRPRLSD
                     ARFFFEQDRKAGLSSRVGKLDSVVYHNKLGSLGDRVQRLDAVARAVAVRLGADDAQAG
                     QAARLCKADLVTDMVGEFPELQGIMGRYYALHEGAQAVVADAIEQHYRPRFAGDALPD
                     SNIGRALALADKLDALVGFFGIGQVPTGDKDPFGLRRAALGVLRILMEAPLPLDLAAL
                     IADAVASFPPGVLTAEVDAPLFDFMLERLRNLLRDAGHAPAAVDAVLALRPTRIDLVV
                     PKLEAVAAFQQLPEAQALAAANKRIVNILRKAGGEFAEPEVALLAEDAEKALFHAIVD
                     VAPLARSHFQNEAYTDALRVLAGLRAAVDRFFDEVMVMAEEPLVRQNRLALLNQLAGL
                     MNQVADISRLSA"
     gene            complement(47056..47964)
                     /gene="glyQ"
                     /locus_tag="PLH85_03000"
     CDS             complement(47056..47964)
                     /gene="glyQ"
                     /locus_tag="PLH85_03000"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNB63554.1"
                     /translation="MSVHKPTFQQIILRLSNFWAERGCTLLQPYDIEVGAGTFHTATF
                     LRAIGPEHWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPSPANIQELYIDSLR
                     ALGIDPNAHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQEVGSLSCKPVLG
                     EITYGLERLAMYLQGVENVYDLVWSIGPDGTPVTYGNVYHQNEVEQSTYNFEHSNVEW
                     LFRLFGEYEAEAKRLMAVPLVLPAFEMVMKCSHTFNLLDARGAISVTERAAYIGRVRA
                     LAREVAQAYFNSREALGFPMLKKEAA"
     gene            48198..49823
                     /locus_tag="PLH85_03005"
     CDS             48198..49823
                     /locus_tag="PLH85_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB63555.1"
                     /translation="MNLSELRVATRMRILVALTLIGLVALCTVSLFRLHASMMDDRKQ
                     QTRYVVQLGLGVLQHFHALAQSGALSDADARKAAIETLRTLRYEGTNYIFVVSTTSHY
                     ILMPPRPEKEGTDASGTKDSTGKAIIPELVKAALAGGGFVDYLFPKPGGTDPQPKVSF
                     AAQFGPWQWVLGTGIYVDDVDVEFRAIAWTLGGISAVLLIVLGVAGWGVGISIIRQLG
                     GEPREATAIMQQVADGDLSLRLEDSRPGSMLHALSTMIASLRTMMGEINRSAVQLVSN
                     ADHISQVSGEVADAAIRQSDATAAMAAAMEELTVSSSHISSSARDTEDNSQEAMRLAA
                     EGTQRVQQASGAIRKMSETVLGASTRIRALEGRISQVSSIANVIKEIAGQTNLLALNA
                     AIEAARAGEQGRGFAVVADEVRKLAERTSSATTEIEQMIVGIQSDTGSAVEAMNAALP
                     EVDEGVSLAASAAESLAAIENGATRTLERVHEIADATREQSAASTAIAQRVEDISNMV
                     ENTTKTIRGAAEAAVGLERIAGSLRDQVSHFRV"
     gene            complement(49820..52504)
                     /locus_tag="PLH85_03010"
     CDS             complement(49820..52504)
                     /locus_tag="PLH85_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNB63556.1"
                     /translation="MAPERAAAWHARSPQDVLDALRSSPHGLTTADAAERLRRYGPNR
                     LPGRPGPGPWQRLAQQFHQPLIYILLASGAIALLLGEWADAGVIFGVVLVNALIGFVQ
                     EGKAADALAALARSVASAVTVLRDGRMVACEAVELVPGDVVRLGAGDKVPADLRLFDA
                     RSLQIAEAALTGESVAVAKDTATVPADGVLAERACMAYAGTLVTAGQGCGVVVATGAD
                     TEVGRIAHLLDVTDALATPLTRAMAGFSRVLLWLILGLAVFGFVVGWMRGEALADMLM
                     AAVALAVGAIPEGLPAAVTVTLAIGVARMARRKAIVRRLPAVETLGSTTVICSDKTGT
                     LTENRMTVKVLWQGGRSFEWAENTPEPSAEPGRAGLPAGLNELLVAGVLCNDARLQAD
                     AAGGPASGDPTEVALLVAADVGGLDGAALRGACPRLGEIPFDAARPYMAVKVCVADHP
                     VVFVKGGLEALLPRCAAQIGVDGCPEALDPAAVEVAATALAADGLRVLAFARGGDGTL
                     GAELPAQGLVFLGLQAMQDPPRPAAIRAVAACRAAGVTVKMITGDHAATALAVARQLG
                     IAADGDAVLTGRDLAGLDALALQAAAMRVNVFARVEPAQKLRLVQALQAGGEVVAMTG
                     DGVNDAPALKAADIGVAMGAGGTEVAKEAAAMVLTDDNFATLEAAVEAGRGVFDNLVK
                     FILWTLPTNFAEGLVILFAVLAGTTLPITPLQILWINMTTAVLLGMTLAFEPIEPDVM
                     GRPPRPPGAPVLDAVQMRRVVLVGALLVAAAFGLFLRWRGFGASLEEARTIAVNVFVA
                     GEVAYLFNCRSLRGGFWRGGVLANPWFWLGIGAMVALQAAFTWWPAMNRLFASAPPPP
                     AAWLEIGAAAWALALAVGLEKRLTGGSASRPGAARIAR"
     gene            complement(52504..53877)
                     /locus_tag="PLH85_03015"
     CDS             complement(52504..53877)
                     /locus_tag="PLH85_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNB63557.1"
                     /translation="MSDQIKYLLDESRLPKAWYNIAADLPVPLPPPLHPGTLQPLGPA
                     DLAPLFPNAIIAQEVSTERYVDIPEPVRDVLRQWRPSPLFRARRLEKLLDTPAKIYYK
                     YEGVSPAGSHKPNSAVPQAWYNAQEGVKRLSTETGAGQWGSSLAYAGGLFGLDVTVFQ
                     VRVSYDQKPYRRALMETWGARCIASPSLETESGRAVLAKTPDHLGSLGIAISEAVEVA
                     AKRDDTKYALGSVLNHVLLHQTIIGQEAIAQLEMAGDDPDVVIGCTGGGSNFAGIAFP
                     FIGLQLRGGANARRRRILAVEPSACPSLSRGQYAYDFGDTAHLTPLVKMHTLGSEFTP
                     PGFHAGGLRYHGMAPLVSHVKELGFVEARNYHQNACFEAGVMFARSEGIVPAPEANHA
                     IRAAIDEALRCKAEGRSETILFSLSGHGHFDMASYTNYLAGKLVDQDYDEQELAMALS
                     GLPRVPV"
     gene            complement(53982..54380)
                     /locus_tag="PLH85_03020"
     CDS             complement(53982..54380)
                     /locus_tag="PLH85_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNB63558.1"
                     /translation="MMRPYPTNSPEALARLVALAILADGRLHNQEVNWIREHDTASLI
                     GVEQDTLVQVLLDCCRDLLSEGGRGRVDLLSEARLAPLAEEIADPALRKVAISAVLVV
                     AKSDGRLSSEEQTLLRFLLSRWDIELQELV"
     gene            complement(54448..55944)
                     /gene="lnt"
                     /locus_tag="PLH85_03025"
     CDS             complement(54448..55944)
                     /gene="lnt"
                     /locus_tag="PLH85_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNB63559.1"
                     /translation="MSRLRPVLAWAAGAASVLAYAPFEYFPLAAVALAVLYALLGRAA
                     DAAITPGARRRAGFLIGWAWGFGALGAGVSWLYVALHRFGGMPMPLAGSAIALFCAVM
                     ALFAGGAGALFADLRRGRPWADAALFGALWLLTEWTRGWLFTGFPWLVSGYSQTPPSP
                     LAGFAPVFGVFGVGGLLAWIAALLAEAVTRRRIACAGLVLLVGAGGAALRPVAWTVPV
                     GAPLQVALLQPNMEQDLKWRPDMLPRWLDIHLELLRNNPATLVVLPETTLPLLLEHLP
                     DGYLDALTRSAAVGGDAILGAFVRDGDGRIFNAAVSVGRSPTQHYAKHHLVPFGEYSP
                     PLFRWFYDLVHIPMADQTRGAEVQPPMSLAGQKIAVNICYEDVFGEELIRALPEATLM
                     LNLSNLAWYGDSFAQPQHLQIARLRALESGRPMLRATNTGMTGLVQPDGTVSAALPAF
                     TRGALQVAVRGYTGSTPYGRWGNGPAVGLAALVVLVVLAGRVRGRTPI"
     gene            complement(55944..56786)
                     /locus_tag="PLH85_03030"
     CDS             complement(55944..56786)
                     /locus_tag="PLH85_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNB63560.1"
                     /translation="MEPSSSKPSLLERLSALLTREPEDREELLHLLHAAHERNLFDAD
                     ALAIIEGALQVSDMRVADVMIPRAQMDVVRVDDSIEDITRFAITAAHSRFPVIGEGKD
                     DVIGILLAKDLLRVFAGEAFDLRDMLRPAVFVPESKRLNVLLREFRVSRNHMAIVVDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDEAADNIRLDQAGRYRVKAGTEIDDFNAAFG
                     TTFSDDEYDTVGGLLIRKLGRLPKRNEVIVLDNLRFQILRADSRRVYSLLVERTPQAG
                     VIGG"
     gene            complement(56859..57341)
                     /gene="ybeY"
                     /locus_tag="PLH85_03035"
     CDS             complement(56859..57341)
                     /gene="ybeY"
                     /locus_tag="PLH85_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNB63561.1"
                     /translation="MTASALPRRLDLSVQYACHRDGVPLRADFRRWLRAAESGAARIT
                     VRIVDEAEGRELNRDYRGKDYATNVLTFAYAEGEDMPMPEGLPLMGDLVLCRSVVERE
                     AAEQGKPLAAHYAHLVVHGMLHLQGFDHEADADAELMEARERAILAELGYDDPYALEH
                     "
     gene            complement(57338..58297)
                     /locus_tag="PLH85_03040"
     CDS             complement(57338..58297)
                     /locus_tag="PLH85_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB63562.1"
                     /translation="MKNKALEVAFEPADNTRLANLCGVLDENLRQIATAYDVEIARRG
                     ERFSVRGHLVQARLAAQALQHFYDLAGEHLSIDTVQLGLIELANRSQASHSAPALLTR
                     KTELHGRTPRQVDYIRQIQEHDITFGVGPAGTGKTYLAVASAVDAFEREQVERIILTR
                     PAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFDRVAKLFERGCIEIAPLAFMR
                     GRTLSRAFIILDEAQNTSPEQMKMFLTRIGIGSRAVITGDLTQVDLPRGQRSGLGEAL
                     QVLADVRGVAFTRFLKEDVVRHPLVARIVAAYESHSATPAPTT"
     gene            complement(58346..59680)
                     /gene="miaB"
                     /locus_tag="PLH85_03045"
     CDS             complement(58346..59680)
                     /gene="miaB"
                     /locus_tag="PLH85_03045"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNB63563.1"
                     /translation="MKKLYIRTFGCQMNEYDSDKMADVLGAGETLVKTDNPEEADVIL
                     FNTCSVREKAQEKVFHDLGRVKHLKQKNPGLIIGVGGCVASQEGEAIVQRAPYVDLVF
                     GPQTLHRLPKLIAERKATGRPQVDISFPEIEKFDALPPARTEGASAFVSIMEGCSKYC
                     TFCIVPYTRGDEVYRPLADILAEVAGLTAQGVKEITLLGQNVNAWRAPLDGAAGEMAD
                     FAFLLECVHEIPGVERLRYTTSHPREMTARVVDAYARLPKLVSHLHLPVQAGSDRVLA
                     AMKRGYTVLEYKSLVRRLRAARPDISLSSDFIVGFPGETEADFEQTMKLIDELGFDTS
                     FSFVYSPRPGTPAADMADDTPQDLKLARLARLQKAVEAHAARISEAMVGSVQRILVEG
                     PSKKNPAELAGRTDNNRVVNFAGNSRMIGHFVDVTIVSALPHSLRGELVLKD"
     gene            59735..59811
                     /locus_tag="PLH85_03050"
     tRNA            59735..59811
                     /locus_tag="PLH85_03050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59769..59771,aa:Met,seq:cat)
BASE COUNT        10390 a        21575 c        18705 g         9205 t
ORIGIN      
        1 gaaaacgccc ccgacatgcg ggggcgtttt cgtgccggga tcggcgcggg gctgaggtgt
       61 tacatgcgcg cgaccatcgc ctcgccgaag ccggagcagg acacttcggt ggcgccctcc
      121 atcagacgcg cgaagtcgta ggtgacctgc ttgtcgccga tggcggcttc catggccttg
      181 atgaccagat cggccgcctc cacccagccc aggtggcgca gcatcatctc ggcggagagg
      241 atcagcgagc cgggattcac cttgtcgagg ccggcgtact tgggcgcggt gccgtgggtg
      301 gcctcaaagc aggcgtactg gtcagagatg ttggcgcccg gcgcgatgcc gatgccgccg
      361 acctgggccg ccagggcgtc cgagatgtag tcgccgttga ggttggtggt ggcgatcacg
      421 tcgtattcgg ccgggcgcag caggatctgc tgcaggaagg cgtcggcaat ggcgtccttg
      481 acgatgatcg tgcggccggt cctggggttg acgaaggaac accacgggcc gccgtcgatg
      541 ggctgggcgc cgaattcctg ctgcgccacc ttgtagccgg tgtcgcggaa caggccctcg
      601 gtgaacttca tgatgttgcc cttgtgcacc agggtgacgc tcttgcggtc gttggcgatg
      661 gcgtaattga tggcggcgcg aacaaggcgc gtggtgcctt cgatggacac gggcttgatg
      721 ccgatgccgg agctttccgg aaagcggatc ttcttgacgc ccatctcctg ctgcaggaag
      781 gcgatgacct tcttcacgtt gtccgactct gccgcccact cgatgccggc gtagatgtcc
      841 tcggtgttct cgcggaagat gaccatgtcg gtcagctcgg gctgcttgag cgggctgggc
      901 acgcccttga agtaacgcac cggccgcacg cactggtaga gatcgagctc ctggcgcagg
      961 gccacgttca gggagcggat gccgccgccc accggcgtgg tcatggggcc cttgatggag
     1021 acggagaatt ccttgagggc gtcgaaggtc tccttcggga accattcatc gggaccatac
     1081 agctgggtgg atttttcgcc ggcgtagacc tccatccagt ggatcttgcg ctggccgccg
     1141 taggccttgg tgactgcggc atcgatgacc tggatcatca ccggggtgat gtccaccccg
     1201 atgccgtcac cttcgatgta ggggatgatg ggattgtccg gcgtcggctg gcccgggacg
     1261 attttttggc cgccggcagg caccttgatc agtgaagtac tcattccagt tccctcgctc
     1321 gtaattcgat ggaaaggcag atcggccgat accgggggca agcccgaggt gacacgcgta
     1381 acgcggcccg caccggcgcg atcgcagcac tgccgacccg cccggtggtg tggacaggca
     1441 gggtctggcg gcgacgatta tggctgaaat gcatgacgcc acaaagcgcc ggaaatgctg
     1501 ctgtgcagcg cgatccgggc gtttcgcctc ccttccggaa ggcccgtcgg gcgatgctaa
     1561 gatgcagcct cgaccctgtc gcggcgatgt acccgcgccc tcgcgcgaac gggccgaatg
     1621 gagagtgtgc ggaggctgaa tggacaccca cggcaacgaa aaccccgtcg cccaggccat
     1681 cgcccaggcg atgatcgagg gcttcaacaa gcactaccgg atcttccggg agacctcgcg
     1741 ccatgcaaag gagagcttcg aggccgccga ctggcaggat cagctctcgg cggtgcgcaa
     1801 ccgggtgcag ttctacgatg accgggtcaa cgagaccgtg cagcgcctgc acgaggaatt
     1861 cgacgcggaa tccatcgatg acgccacctg gcaggccgtc aagctgcact acatcggcct
     1921 gctcctcaac cacaagcagc cggagctggc ggagaccttc ttcaactccg tcaccaccaa
     1981 gatcctgcac cgggattact tcagcaacga ctacatcttc acccgcccgg cgatctcgac
     2041 ggattacatc gagtccttcc cgcccaccta ttccagctac tacccccagg acgaaggcct
     2101 gcgcgccacc gtgcggcgca tcgtcgagga tttcgactgg cagcgcagct tcgaggatct
     2161 ggagcgggac gtggattacg tgctgcgcac cgtcgagacc catctgggcg gctggcccag
     2221 gatggaggtg aattgccaga tccaggtgct gtactccgcc ttctaccgca acaagaccgc
     2281 ctacatcatc ggcaagggcc tcaacggcta ccaggaaatc cccttcgccc tcgccgtgcg
     2341 ccacggtgcc tcgggcatgc tctacctgga caccatcctg ctcgacacct ggcgcatctc
     2401 cctgctgttc tccctgtcgc gggcctactt cctggtggac atggaggtcc cctcaggcta
     2461 cgtgcagttc ctgcgcagca tcatgcccaa caagccgcgc tcggagctct acaccatgct
     2521 gggcctgggc aagcagggca agaccagctt cttccgcgac ctggtgtcgc acctgcgcca
     2581 ttcaaacgat cagttcatcg aggcaccggg catccgcggg ctggtgatgc tggtgttcac
     2641 gctgccgtcc tacccctacg tcttcaagat catcaaggac accttcggca gctccaagaa
     2701 catggaccgg gccacggtca agcgcaaata cctgctggtg cgtcaggtgg accgggtcgg
     2761 gcggatggcc gacacgctgg aattctccaa cgtggccctg ccccgcagcc gcttccatcc
     2821 ggatctcatc ctgcagctgc gcaccctcgc cccgtcggtc atcgaagagg acggcgacac
     2881 cctggtggtc aagcacctgt acatcgaacg gcgcatgacg cccctcaacc tctacctgga
     2941 caaggccagt gacgagcagg ttgaggaggc cgtcaaggag tacggccagg ccatcagcga
     3001 gctggccatc gccaacatct tcccgggcga catgctgtgg aagaacttcg gcgtcacccg
     3061 ctacggccgc gtggtcttct acgactacga cgagatcgag tacatgaccg actgcaactt
     3121 ccggcggatc ccccccgccc ccaacgagga catggagatg tccggcgagc cctggtattc
     3181 cgccgggccc cacgacatct tccccgagga gttcgccacc ttcctgctcg gccagcccaa
     3241 gatccgcaag gccttcctca agcatcaccg ggccctgctc gatcccaggt tctggcagga
     3301 ggcccaggag aagatccgca acggctatgt ggaggatttc tacccctatc cgcaggcatt
     3361 gcgcttccgc acgctctacg ccgagcgtcc tgacgaacca ccgcccgcgt gacgccgtcg
     3421 ccccagccgc cgttcccggc ggacggccat cagggctgac cctattcacg taaaccccag
     3481 tggccgcgcc tggatttcgc gcccactatg catgccgatg cgacaccctc gcagcaatcc
     3541 ggcatccata ccaatagggg agacaatcgt gcaaaacagc tttcagatcc gggcccttgc
     3601 cgccgccatc ggcagcgccc tcgtcctggc cgcctgcggt ggcggcggca gcaccggcta
     3661 cagccagatc aacggcacgg cggcagaagg cacggccatc gccaacagca cgctgaccgt
     3721 caaggatgcc agcggaaaga cccgcagcgt gaccaccgat gcctcgggca attacacgct
     3781 cagcctcgcc gatctcagct tccccttcat gctccagatc actggcagcc agggcacctg
     3841 gcatgcgctg gtgagcagcg acgacgtggg caagccggtc aacgtcaata acgccaccga
     3901 cagcattgcc cagctcgccc tcggcgtctc cggcaacgcg gccctgcgcg cggccttcac
     3961 cgccggcagt ttcaagagcg tcaccggcca ggccatcgcc tccgccgaca gcaagctgct
     4021 ggacgccctc gaagcggaac tcggcaagcg gccggagagc ctgcgcagcg ccaccttcac
     4081 ccccgccacc gacagcactc cgggcgacga aaccgaccgc ctgctcaccc tcgtggatgc
     4141 ccagcccagc ggcagcggct tcgccacttt caaccgcgtg cccgagttcg tgtggaccga
     4201 cagcatcgtc accaagacct atgacggcac caccgacgac ctgctcaccg ccggcctcgg
     4261 caagaccggc ctgacgtccg ccacggctcc ggcctacgtc aatgcggcct cgccaaccgc
     4321 cgccgagctg cgccgcaatg ccatccacac caactaccgt gcgctggtgg acgccaacaa
     4381 gaccgccggc ggctggacca ccctgtacgg ccccaacatc gacaccagcg gcaacgacac
     4441 cctgggcgaa ggcaggatcg ccggcgacga gaccatcgcc tattcgggcg actccagcgg
     4501 caagcgcaag gtcacgctga tggtccaggt gccggccagc tttgacaaga ccaagccctg
     4561 catcgtcacc gccacgtcct ccggctcccg cggcatctac ggcgccatcg gcaccgccgg
     4621 cgactggggc ctcaagcacg gctgcgccgt cgcctacacc gacaagggca gcggcaacgg
     4681 catgcacgac ctggccgccg acaccgtcaa cctcatcgac ggcacggcca gcagcagcgc
     4741 cagcgccggc aagcgcgccc tgttcgccgc caatctgagc aaggccgaac tggacgccta
     4801 caacctcaag agccccaacc gcatcgccta caagcacgcc cactcccagc agaaccccga
     4861 gaaggactgg ggccgcaaca ccctcgacgc cgtgggcttc gccttctacg tgctcaacga
     4921 gaagtacggc accgtcagca atggcaagaa gctgcgcgat ctgaccccca ggaacacact
     4981 cgtcatcgcc tcctccgcct ccaacggcgc cggcgccgcc ctgcaggcgg ccgagctgga
     5041 caagctcggg ctcatcgacg gcgtggccgt caccgaaccg cagatccagc ccaaggacgt
     5101 gagcagcctc accatcaaac agggcagcaa caccgtcgcg acgatcggca agcccctcat
     5161 cgactacttc acctacgcca acatctacca gccctgcgcc gccctcgcca cggcagcaag
     5221 cgggtcgccc ggcgcaagcc tcgttgcctt ctacgccacc aaccggtgca cgtccctcaa
     5281 ggaaaagggt ctggtgagcg gcaccaccac ccaggaacag gccgacgacg ccctccagaa
     5341 gctccatgcc tacggctggg ccgcagagca ggacgtgctg cacgcctccc atcacgccct
     5401 ggccacgccc tccatcgtcg tcacctacat caacacctac gggcgcttca acgtgaccga
     5461 cgaagtgtgc ggcttcacct tcgccaccac cgtcgcctcc ggcgccaccc tcggcaacgt
     5521 caccgccacc agcgccctgg tccaggccgg catcttcgcc accggcaacg gcgtcccgcc
     5581 cacagccggc atcaacctgg tctataacga ctcggtcggc ggcgccaagc gcgatgtgct
     5641 cgccatctcg ccctccacca accgggccga cggcgccctg gacggcgccc tgtgcgcccg
     5701 ctcgctggtc accggcagcg acccggtcac cggcgccaag ctcacgggca cgctcctcgc
     5761 ccagtcccag cgcgtgcgtc agggcatcga cgaggtcctg ttcaagggcg aactgggcgg
     5821 caagccggcc atcatcgtgt cgggccggtc cgacaccctg atcccggtca atcacgcctc
     5881 ccgcgcgtac tacggcatga gtcagaaggc agacggcacc accagcaggc tgcgctacta
     5941 cgaggtgacc aacgcccagc acttcgacgc cttcatcgac aacaacgcgg ccgtcgccgg
     6001 ctacgacacc cggcttgttc ccctgcatgt gtatttcaac cgcgccatgg acctgatgta
     6061 cgcctacctc aagaccggcg ccgcgttgcc cgcctcccag gtgatccgca ccacgccgcg
     6121 gggcggtacg gctggcgccg caccggcgat cagcgccacc aacgtgcccg ccatcgccac
     6181 caccccggca gccggtgaca ccatcaccta cagcagcggg acgctcagca ttccggactg
     6241 atccgtatcg cacgcagcat gaaaaagggc gaagccacgg cttcgccctt tttcgtctga
     6301 atcgcctcgc ggcgctccgc tcagtcccag ccggactcca tcagccatgg gccgtcaccg
     6361 gccgccagcg cgtcccgcac atagggcacc agcgccagcg gcagtgcgtc gcgcccgaac
     6421 caggccagat cgtcgcactt gtccggttct gcaatgcgcg gctcaccgga ccacgtcagg
     6481 gcccgcacga agaagtcgat ccggttggtg tccgagcggc gatgcaccac ccccagcagc
     6541 gccactgcgt ccggcgccag ggtcacaccc agttcttcgg ccatctcgcg atgggctgtc
     6601 atgccgacgg actccccctc ctccacgtgt ccgccgggca gactgtagag tccgtcgaag
     6661 aagccggtgc cggcccggcg catcaacagc acccgactgt cccgctcgaa cagcacatgc
     6721 acgccggtgg ggatgccgcg acgactcaaa tcagtgcttc ccggtgctgc cgaagcctcc
     6781 cgcgccacgc tcgctgacgt caaaggactc gacaagattg aaggccacct gcagcaccgg
     6841 caccaccacc agctgggcga tgcgttccat gggctggatc gtgaagctgt cgtggccccg
     6901 attccatacg gacacgaaga tctgcccctg gtaatccgaa tcgatcaagc ccaccagatt
     6961 gcccagcacg atgccatgct tgtgccccaa ccccgagcgg ggcaggatca tcgcggccag
     7021 gccagggtcg gccagatgga tcgcgatgcc cgtcggcacc agcagcgtcg cgccgggcag
     7081 cagatgggcc ggcgcctcca gacaggcgcg caggtcgagc cccgcggcgc cgctggtggc
     7141 gtaggccggg ggctgttcct tgaggcgtcc gtcgaggatc cggatgtcga tgctgtgcat
     7201 gggtgtgagt ctccgctgac gaattcagga aagtggcggc aacaggcctg ccagatgcgc
     7261 gacgatggcg cgggccacct cgggtttggg cgcccgttcg agcgggtgac gacccgcctc
     7321 gtcatacagc acgacctggt tgtcgtcgct ccccagacca tcctggacca gattgccgac
     7381 caccaggggc agcttcttgg cgcgtcgctt gccttccgcg tattcatcca ggttccggct
     7441 ctccgccgca aagccgacgc agaagggcgc atccgcgcgc gcggccacct cggcgaggat
     7501 gtccggattg ggcgtcaggc tgatctgcag ggccgcaccc gacttcttga tcttgtgctc
     7561 cgccgacgta tccggccggt aatcggccac cgcagcaacg ccgatgaaca cgtccgcacc
     7621 gggcagcgcg gcgaacaccg catcgcgcat ctgcagcgcg ctcctgacgc tgatccgtgc
     7681 cacgccggcc ggcaccggca gcgcgaccgg gccactcacc agggccacgt ccgcccccgc
     7741 ctgagcgcag gcgcgggcca gggcatacgc catcttgccg gaacttgaat tggtgatccc
     7801 gcgcaccggg tcgatggcct cgaaggtcgg ccctgcggtc atcaccacgc gccggccggc
     7861 cagcagcttg gactggaaga agccgatcaa tgcctcgcac aggtcctccg gctccagcat
     7921 gcgcccaagg cccacctcgc cacaggcctg ctcccccgcc gccggaccga acagggtcac
     7981 cccgtccgcc gccagttgca cggcgttgcg gcgggtcgca ggatgctccc acatctgccg
     8041 gttcatcgcc ggcgccacca gcagcgggca gtcccgcgcc aggcacagcg tcgtcagcag
     8101 atcatcggcg cggccctggg cgagacgcgc catcacgtcg gcggtggccg gcgcaatgag
     8161 gatcgcgtcc gcgtcccgcg acaggtcgat atgggccatg ttgttggcca tgcgcggatc
     8221 ccacaggtcg gtccagaccg ggcggccgga caaggcctgg aaggtcaccg cggtgacaaa
     8281 gcgcgcgccc ccctcggtga gcaccacgtg cacctccgcc cccgccttgc ccagcagtcg
     8341 caccagttct gccgccttgt aggccgccac gccgccggtg acgcccagca cgattttcct
     8401 gcccgaaagt tcagccatgc cgatggtatg atccgcaatc ctctggaatc cggattctac
     8461 accgatggcc atcaccgact ggcccgcgga cgaacgcccg cgggaacgcc tgctcgccca
     8521 cggcgccgaa gtactgtccg acgcggaact gctcgccctc ttcctgcgcg tcggtgtgcg
     8581 cggcaagagc gcggtggacc tcgcccgcga cctgctcggc cgctttggca gtctgtcggc
     8641 cctgtgcacc gcatcggtgg acgacttctc cacgatcccc ggcatgggcc cggccaaata
     8701 cgcccagttg caggccgttc tggaactggc ccgccgtgcc ctcagcgaag agatggccag
     8761 ccgcgacgtc ctcgattcgc cccgcgccgt gcgcgactgg ctgcgcctca agctcgccgg
     8821 tctgccccac gaagtcttca tggtccttct gctggacgcc cagaaccggg tcatcgccag
     8881 cgaggaattg tttcgcggca cccttgccca gaccagcgtc tatccccgcg aggtggtcaa
     8941 gctggccctc ctgcgtaacg ccgcggccgt gatcctggcc cacaaccatc catcgggcgt
     9001 cggcgaaccc agccacgccg accagctcct cacccgcagc ctcacccagg ccctggcgct
     9061 catcgacgtg cgtgtgctgg atcacttcat cgtcgctggc accgcgtcgc cggtatcctt
     9121 cgcggaacgc ggcctgctgt gacgtgctga taggaccgcc ccatcaaagg cttgcatcat
     9181 cttcacaagc tttgatagac ttaagggctt accgattcaa aattgtttgg agcaacacat
     9241 ggctcgcgtc tgtcaagtaa ccggaaaagc accgatggtg gggaacaacg tctcccacgc
     9301 caacaacaag accaagcgtc gtttcctgcc caacctgcag aaccgcaagt tctggagtga
     9361 ggccgagaac cgctgggtgc gcctgcgcgt gaccaacgcc gccctgcgca ccatcgacaa
     9421 gaaaggcatc gacaccgtca tcgccgagct gcgcgctcgt ggcgagaagg tctgagcctt
     9481 tcacccactg gagataaacc accatggcaa aaggcggccg cgaaaagatc aagctcgagt
     9541 ccactgctgg caccgggcac ttctacacca ccaccaagaa caagcgcacg acgccgaaca
     9601 agctggaatt cagcaagtac gaccccgtcg cccgcaagca cgtgccgtac aaggaagtca
     9661 agctcaagta atcgctcccg gtacgcgatg actgcttgtt gcacctgaaa gacccgccca
     9721 gagcgggtct tttgctttgc gcggcgcaac gccagtttgc gcccctacgg cctgaccgcc
     9781 ccctgatcca tcgtcaaccg cgacacaatc ttgcaaatga aaaccgccgg aatgcagatg
     9841 cacccggcgg tttttcacga acccttcctc acacgccccc caaacactgc accaaaacag
     9901 tgcccagggt gcgtcgagag atcaggcacg ctggatattc gacgcctgct tgcccttggg
     9961 gccctgcgtg acctcgaact gaaccttttc cccctccttc agcgacttga atccgctcat
    10021 ggaaatcgcg gaaaagtgcg caaacagatc ttcgcttccg tcgtccggcg taatgaagcc
    10081 gaagcctttg gcgtcgttga accacttaac agtaccagtt gccatagtgc agtttccttc
    10141 ttggttttca gtcatacaga accgggtctc cggctgagtc tgcagcgggt gctcgtggac
    10201 ggttggcgta atacccatgc ccgacggctt ttccccaaaa gcccccgcgg caaagcgcca
    10261 accatcctcc cgacgcagac acggcttcgc ttttacgtca cttgcccccc accgtcaacg
    10321 gtccagtcgc agcattttca tgctgcaata cagcaggatc gcgaccaatc gcgtcaaaat
    10381 ttcgcagcgc ctttcaaggc ggacgcggca ctccggagcg ctgaaaatgg ccgtgcaacg
    10441 caccatccga gggcctctgc accaacacga atcagtgcca aatcgtgtgt caattctctc
    10501 ttgcacaatc atcgaggttg tttagaatgg gccgcatggt cacaaaaaag cagggcgaat
    10561 cagtacttga aacggaggtc acgcggatca aaccgccccc cttgttcaag gtcatgctgc
    10621 tgaatgacga cttcacaccc atggacttcg tcgtgactgt catcgagaaa ttctttggac
    10681 tggaccgcga acaagccacg cggatcatgc tcaaagttca cagagagggc gttggcgtct
    10741 gcggcatctt cccccgggac atcgcagcca ccaaggtcga gcaggtcatc gcgtacgcgc
    10801 gccagaacca gcatcctctg gcctgcgtca tggaggaaaa ctgaaatgat cgcgcaagag
    10861 cttgaagtca gtctgcacat ggccttcgtc gaagcccggc aaaagcgtca tgagttcatc
    10921 accgtcgagc acctcctgct cgcgcttctc gacaatcctt ccgcagccga agtgctgagg
    10981 gcctgcgcgg cccgcagcga cgaactgcgc aaggaactga ccaatttcat caacgagcac
    11041 acgcctcgcg tcgaaggcac agaagacatc gacacccagc ccacgctcgg cttccagcgg
    11101 gtgatccagc gcgccatcct ccatgtgcag tcctccggca agaaggaggt caccggcgcc
    11161 aatgtgctcg tcgcgatctt cggcgaaaag gactctcacg cggtctattt cctgcaaaga
    11221 cagaacatct cccgcctgga tgtggtgaat ttcatctccc acggcatcac caaggcaccc
    11281 cagtccggcg ccagtcccgc gcgcccgaac gaatcttcgg aacagggtga ggccgagccg
    11341 gaaaacccct cggcgggcgg cgcactggac agcttcacgc agaatctcaa ccagcaggcg
    11401 ctcctcggca agattgatcc gctcatcggc cgagagaagg aagtggagcg ggtcatccag
    11461 accctctgcc ggcggcgcaa gaacaacccc ctgctggtcg gcgaggccgg cgtcggcaag
    11521 accgccatcg cggaaggtct ggcgcgacgc atcgtcgaag gtcgggtgcc cgagatcctt
    11581 gcggatgccc aggtgtacgc cctcgacatg ggcgccctgc tggccggcac caaatatcgt
    11641 ggcgacttcg agcagcgcct caaggcggta ctgaagcaac tcgtcgatca caacaacgcc
    11701 atactgttca tcgacgagat ccacacactg atcggtgccg gcgcggcatc cggcggcacg
    11761 ctcgatgcat ccaacctgct caagccggcc ctgtcgtccg ggcagctcaa gtgcatcggc
    11821 gccaccactt acacggagtt ccggcagatc ttcgagaagg atcacgccct gtcgcggcgc
    11881 ttccagaaag tggacgtggt cgagccctcc gtccccgaga ccgtggagat cctcaagggc
    11941 ctcaagtcgc gcttcgagca gcaccacggc atcaagtatt ccgcttcggc cctgtccagc
    12001 gcggcggaac tctcggcccg ctacatcaac gaccgtcacc tgccggacaa ggccatcgac
    12061 gtcatcgacg aagccggcgc cgcccagcgc atcctgccca agtccaagca aaagaagctc
    12121 atcggcaaga ccgaaatcga ggagatcgtc gccaagatcg cgcgcattcc gccgcgcaac
    12181 gtctccaacg acgacaaggc ggccctgcgc accctcgaac gggatctcaa gaacgtggtc
    12241 ttcggccagg acgcggccat cgaggccctc gccaaggcca tcaagatgtc ccgctcgggc
    12301 ctcggcaatc cgcagaagcc catcggttcc ttcctgttca gcggccccac cggcgtcggc
    12361 aagaccgagg tcgcccgcca gctcgcctac accctgggca tcgagctggt gcgcttcgac
    12421 atgtctgaat acatggagcg tcacgcggtg tcccgcctca tcggcgcgcc gccgggctac
    12481 gtgggctttg accagggcgg cctgctcacc gagcagatca ccaagaagcc ccactgcgtg
    12541 ctgctgctgg atgaaatcga gaaggcccac ccggacatct acaacatcct gctgcaggtg
    12601 atggaccacg gcaccctgac ggacaacaac gggcgccagg ccgacttccg caacgccatc
    12661 atcatcatga cgaccaatgc gggcgccgag accatgcaga agtcggtcat cggcttctcg
    12721 gccaagcgcg aggtcggcga cgagatggca gagatcaagc ggatgttctc gccggaattc
    12781 cgcaaccggc tggacgccac catctccttc aagtccctcg accacgacat cgtgatgaag
    12841 gtcgtggaca agttcctgat gcagctcgaa gcccagctcc acgagaagaa ggtggacgcc
    12901 cacttcaccg accgcctcaa ggaatggctc ggccagaagg gcttcgatcc gctcatgggc
    12961 gcacggccga tggcgcgcct catccaggac accctgcgcg ccagcctggc cgacgagttg
    13021 ctcttcgggc gactggccgg tggcgggcac gtcaccatcg acctggacga caatggcaag
    13081 gtggccctgc agttcgagga agaggccgac gtggccacag tctgatccgg acggcctaca
    13141 atcgcgggcg gcttcggccg ccctttttcg ttgacctgtt taagaccctc ccccaggaaa
    13201 ccgacatgac tttccaaacc accgatctgt gcgacgcgaa tgaaggcaag gtccgcgtcg
    13261 tcgcccccat gttccgcagc ttcggcggca agacccgctt cgccggcccc atcagcaccc
    13321 tcaaggtgtt cgaggacaac tccctggtcc gctccgcgct ggagaccgcc ggcgcgggcc
    13381 gcgtgctggt ggtggacggc ggcggctcga tgcgctgcgc catggtgggc gatcagctcg
    13441 ccgcactggg cgtgaagaac ggctgggccg gcatcgtggt atacggctgc atccgcgact
    13501 cgggccccat cgctggcatg gacatcggcg tgttcgcgct gggcacccat ccgcagaaga
    13561 gcgtcaagaa gggcgcgggc gaccaggata ttccggtcac cttcggcggc gtcacctttg
    13621 taccgggaca ctggctgtac gcggatgagg atggcgtgat cgtcgccgac gcgccgcttg
    13681 tgtaagactt cacacacaac gccgtgcagc gcagcgactt tttatttcac ctgcgctaac
    13741 ggcgtttcac aatacaaaaa caacagatct tgtatctgat tttatagaga aatttatttc
    13801 ttatatctta tataagacct gttgctgccg tgcaaaatcc ccccctatac tctcgctcac
    13861 tgaaacgcac atttcacaga gagtttcccc cctcctgtca gaagtgcgac agcgcggctt
    13921 cgtaaccggt cacaggttgc ccgctcccag ttttccctcc ccctcaacgt ttcttaagga
    13981 tgtcgttatg tctctgactc gcgaacagca aatcgccgcc ctggaaaagg attgggctga
    14041 aaacccccgc tggaagctcg tcaagcgcgg ttactccgct gctgacgtcg ttcgcctgcg
    14101 tggcagcctg caacccgagt acaccctggc ccagcgcggt gcccaggtcc tgtgggacaa
    14161 ggtcaacggc gccgccaaga agggctacgt gaacgccttc ggcgcgatca ccgcgggtca
    14221 agccatgcaa caggccaagg ccggcctgga agccgtgtat ctgtccggct ggcaggtcgc
    14281 cgccgacggc aacacctccg aaaccatgta cccggaccag tcgctgtacg cctacgactc
    14341 cgtcccgacc atggtccgcc gcatcaacaa caccttcaag cgcgcggacg aaatccagtg
    14401 gtcccgtggc gtcaatccgg gcgacgaagg cttcatcgac tacttcctgc ccatcgtggc
    14461 cgacgcggaa gccggtttcg gcggcgtgct gaacgccttc gaactgatga agaacatgat
    14521 cgccgcgggc gctgccggcg tgcacttcga agaccagctg gcggccgcca agaagtgtgg
    14581 ccacatgggt ggcaaggtgc tcgtgccgac ccgtgaagcc atcgaaaagc tgatctctgc
    14641 ccgtttcgcc gctgacgtca tgggtgttcc gaccctgatc ctggcccgta ccgacgccga
    14701 agccgccaac ctgatcacct ccgactacga cgagaacgac aagcccttcc tcaccggcga
    14761 gcgcacccag gaaggcttct tccgtgtcaa gaacggtctg gaacagtcca tctcccgcgg
    14821 cgtggcttac gctccgtacg ccgacctggt gtggtgtgaa accggcgtgc ccgacatcgg
    14881 cttcgcccgt gaattcgccc aggccatcca ggccgcctgc cccggcaagc tgctgtccta
    14941 caactgctcg ccgtccttca actggaagaa gaacctgaac gacagccaga tcgcctcctt
    15001 ccaggaagaa ctgtctgccc tcggctacaa gtaccagttc atcaccctgg ccggcatcca
    15061 cgtcaactgg tacaacacct tccagttcgc ccatgcctat gcccgcggcg aaggcatgaa
    15121 gcactacgtc gagatggtcc aggaacccga attcgccgct cgcgaaaagg gctacacctt
    15181 cgtgtcccac cagcaggaag tgggcaccgg ttacttcgac gacgtgacca ccgtgatcca
    15241 gggcggctct tcctccgtga aggcgctgac cggctccacc gaagaagaac agttccacta
    15301 agaagaaatg cctggatcgc cggactccct ctccggcacg cctggttgaa gacccccgcg
    15361 ggtttgcgcc cgcgggggtt ttttcgttga cccacgcgga cggcgaacgt cgcccgttaa
    15421 aatacgtccc cctcccacgc gctgcccggc gctcccatcc ccgccatgca atccctgtgg
    15481 atgatcgctg ccagctttct cttcgcctgc atgggcgtat gcgtcaagct cggcgccttg
    15541 cgcttttcca cgggcgagct ggtgttctac cggggcctca tcgggctgtt gatgatggct
    15601 gtcctgctgc gcgtgcaagg catccctttt gccacgcccc actggaagct ccagctcagc
    15661 cgtggtctgt cgggcaccgt ggccctgatg tgctacttct tcgccctggg catcctgccc
    15721 ctcgctaccg ccgtcacact caactacacg tcgccaatct tcgtcgcgct gatcctcgcc
    15781 ctgtggttcc gcgaacccgt cggcccactg cgccacgcgg ccgtcgccct tggctttgtc
    15841 ggcgtcgtgc tgctcctcaa gcccaccctg caacccgacc agtggcccgg tgcgctgggg
    15901 ggactcggct ccggcctaat cgccagtctg gcttacatca atgtgcgcga gctcggccgc
    15961 gccggagagc ccgaagcccg caccgtcctg tggttttccc tgattaccgc cgccttcggt
    16021 ctgccctggg cgctgggcag cgatcacctg caccgcctcg acgccagcgg cctggccatc
    16081 gtgttgggtg tcggtggatt cgggggctgc gcccagctcg ccatgactcg cgcctaccgc
    16141 tacggcaaga ccatcgtgtc cgccaatctg gcctacacca cggtgatctt ctccagcgcg
    16201 ttcggcgccc tcctgtggga cgagcgcctg ccgtggacct ccttcgtcgc catcgccctc
    16261 atcgtcgcca gcggcgtact ggtctccctc gcctcgcgca aacccgcgcc cgtcgcggaa
    16321 tccgattgaa gccccctgcg tcgggggcta cactggcgat gggttttttc ccctttctcc
    16381 attgactctg aggatccggc gatgatcacc atcacccacg acgcaggcca ggtggacgcc
    16441 gtcgtgttcg gcgaattcac cctctccgac tacaaggaat tcgaggaagt cttcaactac
    16501 acggtgcgct tcgagggccc ggtcaatctg ttcttcgacc tgcgcgccat gagcggcttc
    16561 accgtggaca tggccctcga agagatccgc ttcgcccgcg cccacgctaa cgacttcgcc
    16621 cagattgccg tcgttaccag cgatcaatgg gtcagctgga gcgcctggct gtcgcagctc
    16681 ttcgtcaacg cagatgtccg cgtgttcgac gatgcggacg acgcccgcgc ctggctggcc
    16741 ggggtcgaat cgccggtgca gtgagggtcg cgcacgatga acggcacttt cggcacgctg
    16801 atcagcgccg cggaactggc agaccaggcc gacgcgaact ggcgcctctt cgactgccgc
    16861 ttcgatctgc gcaaccgtcg ctatggacgc gacgcctacg cgagcggaca tctgcccggc
    16921 gccgtgtatc tcgatctgga agaagatctc tccggcccgc tctcgggtct caacggccgc
    16981 catcccctcc ccgacccgca actgctcgcc gccaagctcg gcagcctggg cgtcacgacc
    17041 ggcacccagg tggtcgccta cgacgacgct ggcggcatgt ttgccgcgcg cctgtggtgg
    17101 ctgctgcgct ggctcgggca tccgacggtc gccctactgg acggtggcat ccaggcctgg
    17161 gccagcagtg gacgcgccct cagccaggac gtccctgcac tcaccgcccg cgtgttttca
    17221 ccgcggctgc aacccgctct ccatgtggat gccacgtacg tgcacggcca tctggacgac
    17281 ccggccctgt gcctgattga cgcccgtgcg cctgaccgct tccgcggcga aaacgagacc
    17341 ctcgatcccg tcggcggcca cattcccggt gcgatcaatc gcttctttcg ggacaacctc
    17401 ggcccggacg gactcttcaa accggccgcg caattgcagg aagagttcgg caccctcatc
    17461 aagggcgggg atgcccaccg ggtgatccac cagtgtggct cgggggtcac tgcctgccac
    17521 aacatcctgg ccatggaagt agccggcctg acggattccc gcctgtatgc cggctcatgg
    17581 agcgaatggt gcgccgatcc ggcccggccg gtcgcgaccg gaccgggctg acgccggcgc
    17641 tcgtaacgcc gaaagcctta cgccttgcca aacaggccgc ccagggcaga caggcctgcg
    17701 gaaagcagat cgccgccttg gggcatctgg ccgtcagggg tcaggtgatc gaccacctgg
    17761 ggcagcatct cggccacatg tccggccacc tgatccgcag gcagacccaa ttgcgccgcc
    17821 atgtctccga gccggccacc gagggcctgg ctgatctgat cggcgctcac cggcaggttc
    17881 tgaccggtcc cgacccacga cgccacctgt tcgccgaggc ccgcctggtt cagctgcgcc
    17941 accaggcctc ccagcccgcc ctggccgttc tggatcaggc ccaacacggc ctggagcagc
    18001 atgtgctgca ggtcagaacc gccctgtccc tggccacccc ccagggaacc cagcacctga
    18061 cccgccaatt gatcgagcaa acccatgtcc gtacctcctc aggaaagccg cccactccgg
    18121 cgcctgcgcc gtgcacgggc cgggggctgc gtgccggcca ctctatccca caccgctgac
    18181 ccacatgccg tggacacaga atctgacgcc tgcagggccc cctccgagcc gttaaaatcc
    18241 gtcttttacc gttgagcacc cgccatgacc caggatgaac tcaagcagct ggccgcgctg
    18301 aaggccctcg actacgtgca ggacggcacc atcgtcggcg tcggcacggg ctccaccgcc
    18361 aatttcttca tcgacggcct ggcagcccgc aaggagcgca tcgtcggcgc ggtgccctcg
    18421 tccgaagcct ccgccaagcg gctcgccgca catggcatcc gcctgctgga cctgaacgac
    18481 gtcgccgaca tcccggtcta tatcgacggc gccgacgaga tcgacggcac gctggccatg
    18541 atcaagggcg gcggcggcgc ccagacccgc gagaagatcg tggctgccgt ggcgcagcgc
    18601 ttcgtgtgca tctgtgatgc cagcaagaag gtggcgcaac tgggacgttt tccgctgccc
    18661 gtcgaagtca tccccatggc ccgtgcccag gtcagccgcg aacttgagcg tctgggcggg
    18721 cgtcccgtgc tgcgggaggg ctttgtcacc gacaacggca acctgatcct ggatgtacac
    18781 ggcctgaaca ttcacgaccc cgtcacactg gaaatcgcca ttgatggtat cgtcggagtc
    18841 gtcaccagtg ggttgttcgc caagcgcggc gcagacgtgc tgttgctggc aaccgcgcag
    18901 ggcgtccaga ccttcgaacg cgcctgaaac aaatctgtca tattgcactg atagccttcc
    18961 gcgtctatca gtcaccgagc cgggacatcc atgaacgagc acacctccaa gaagttcgac
    19021 gccgagcttg aaggcatccg gacccgcgtc ctgcagatgg gcggcatggt cgagctgcag
    19081 atcgtcaagg ccatggaagg tctcgcccac ggggatcttc tcctcatcga ccagaccatc
    19141 gagaacgatg cccgcgtcaa cagcctggaa gtcgaacttg acgaattctg cagccagatc
    19201 attgccaagc gtcagccggc ggcggtcgac ctgcgcatga tcagcaccgt ccagaagacc
    19261 atcacagacc tggagcggat cggcgacgaa gccaagaaga tcgccaagat cgccaagcag
    19321 atccacgcct ccgccagccc cttcatgccc cagatccgcc tcggccacat tgccgacgcg
    19381 gtggtggaca tgctgcgcac cgccctcgac gcctacgccc gcctcgacac cgtggccgct
    19441 gcccaggtgg tgcgtcagga caaggacgtg gatgccgagt tcaagggcat catgcgccag
    19501 ctcatcacct acatgatcga ggacccgcgc accatctccc acgccatcga catgatgttc
    19561 gtggccaagt ccgtcgagcg catcggcgac cacgccaaga acatcgccga gtacgtggtg
    19621 tatatggtca agggccgcga cgtacgccac accagcctcg aagaagtgga gcgcgaagcc
    19681 acccgctgaa gcacgccgcc cacgcaccgg aaaagggaac ggcgacgttc ccttttttgt
    19741 tggccggcct tacgttggta tggaatttgc cgttaccgga agagccggcc cgaccggcta
    19801 tcattgcccc tcccggaaac cggatccctg ccatcatgcc gctcaagttc gaactcctgc
    19861 aggccgacga ttattcccag tacctgctgc gggaaaagac cgacatcctc ttcaacctcc
    19921 gtggcctcct gcagaagcgc gcgatgatca gcgccttcat cgacgccagc gcggattcct
    19981 ttctgaccgc cctcctcgcc gtcaccccgg acgaacgcca cctgatcctc gacgccgcag
    20041 ccgacgacag cctcaaccgt cgcgtggaag gggccgaaca gctcatctgc gtcgcccagc
    20101 tggaaaagat caagatccag tttgcggccc gcgagatcgt ccgttttccg ttcgagggcc
    20161 acgaagcctt tcgtgtgcgc atgcccgacg tggtgctgcg cctgcagcgg cgggagtact
    20221 accgtctcac cgcgccgatc tcccacagcc tgtcctgcct catcccggtg gccgagggcg
    20281 agagtgcccg cccccgctcc gtggaagcca gcgtcatcga catcagcggt ggcggcattg
    20341 ccgtgctggt gcccccccag ggcctggcct tcgagccgga gatggagttc gccgactgcc
    20401 gcctgatgtt gcccgagacc ggccccatcg tcacggcgat caaggtgcgc aacctgttcc
    20461 gcatcaccca ccccaacggc caggtgatgc tacgggcggg ctgcgagttc ctcggcctgc
    20521 ccggcaacat gtccaccacc atccagcgct acatcctgcg cacggagcgg gagctcaacg
    20581 cccgacaccg gattcgctga agccccccaa aacgaagaca gccggcagtc gccggctgta
    20641 tcacgtgggg gacgagcctt ccaggcgggc tcagacgctc atgttcatga tgtcctggta
    20701 ggcggtcacc agcttgttgc gcacctgcac catctgctgg aacgacaggt tggccttttg
    20761 caggttcacc atcacgtcct gcaggttcac gttgggatcc tcggacgaaa agcccttggc
    20821 catctcctgg gcctgcatct gcgcggagct cacctgatcc agcgccgact gcagcacctg
    20881 tccgaaatcc gcctgcccgg tcgccgcggc cgcctgggca ggcttgcccg cggccgtctg
    20941 cgccgtggcg cgcaactccg acaacatctg atcgactccg cgggtatcca tgttccggct
    21001 ccttcaatgc ctctccaccg cagcaagacc catgccacgc agggcaatgc acctcatccc
    21061 gggaaaaaac cttcctcccg gtactgctgc agcttgtagc gcaaggtgcg ctcggagatc
    21121 ccgagccgct ccaccgcccg cttgcgcgag ccgcccacct cccgcagcac ctgcagaatg
    21181 tgttcccgct ccagatcctt catgttggcc ggacgcggca attccttgcc gcccggcaaa
    21241 gcctcaccgg caaaatctgc cggcaccggt gtggccgggc gcaatacggg tcgtagcggc
    21301 tccccgttcc agtgaggcag acacagacgc accgtctcgc ccgagacgga ctcgccgctg
    21361 ctcaggatca gggcgcgctg cacggtgttt tccaactcgc gcacattgcc cggccagtgg
    21421 taactcacca gcaccgactc cgcctcgggc gaaaggcgcg cgctgcgacg caggcgcgcc
    21481 ccgtgcagac gggcaaagtg atgggccagc ggcacgatat cgccgggccg ctcccgcagg
    21541 gacgggatcg ccagcggaaa gacgttgagg cggtagtaca gatcctcgcg gaagcgcccg
    21601 gcggccacct ccttggtcat gtcccggttg gtggtggcca ggacgcggat gtccagggcg
    21661 atcgccttct tgccgccgac ccgctccacc tcccgctcct ggagcacgcg cagcagcttg
    21721 gcctgcaggc ccaggggcat ttccgaaatc tcgtcgagga gcagcgtgcc acccgcagcc
    21781 tgctcgaact tgcccgcctg tgcggtggcc gcgccggtga aggcaccctt ctcgtggcca
    21841 aacagggtcg cttccagcag ggtgtcgggg atggccgcgc agttgatggc cacgaagggg
    21901 ccgttgcggc gcggcgactg ctcgtgcagg taacgggcaa acacctcctt gccggtgccc
    21961 gattcaccgg tcaggagcac ggtcgcgtcg gtctcggcca ccttggctgc cagggccagg
    22021 agcttgcggg tgtgggggtc ggccgccacc atgccgcccg ggtcccggtc cggcgcggca
    22081 tagcggcgca cgtgttcgag cagcacctgg ggctcgaagg gcttcatcag gaaatcgcag
    22141 gccccgcccc gcatggcgtc cacggctttc tccacatcgc cgaaggccgt catcagcagc
    22201 accggcagct ggggcaggcg gcggcgcagt tcggcaagca gctcgagccc atccatgggc
    22261 tgcatcttga ggtcggagat caccaggttg aaggcctcgt cgtccgtcct ggccaaggcc
    22321 gcggggccgt cgccgacacc ggtgaccgta tggccggcaa cttccagggt cagacaaacg
    22381 gcgtcccgca ggggcgcatc gtcttcgacg accaggatgt ggagggcatc gttcatgggt
    22441 gatctctcac tgcttcggat ggccgggcgt ccgttcaggc gcccatggcg agggtcagtg
    22501 tgaaggtggt gccccggccc ggttcggacg cgatgccgat gtcgccgccg tgggcccgcg
    22561 ccacccctcg cgcgatggcc agacccagac cggtgccgtc aggccgggtg gtaaagaacg
    22621 gctcgaacag ccgtgactgt aattccggag cgatgccgcg accgtcgtcc gccacgcgga
    22681 agcacgcatg agcaccctcg acaccggcgt cgaagcggat gtgcccgccg gtctcgagca
    22741 cctgcaccgc gttttccagc agattggtga gggcgccggc cacggccttg cgatcaccca
    22801 cgacttccgt acggccacaa tcgcactcga ccgccagcgc gatgcctttg gcgcgcgcca
    22861 ccggctccag ggtatgggcg agttccgcca ccaggtcgca gatcggaaag ggttcacgtc
    22921 ccagggtctc gccgcgggca aacagcaaca tgtcgcggat cagcttttcc agatggcgca
    22981 ggcgctccag ggcgcgcagg ccgatcttgt cccggtcgcc cggtttcagg tcctgctggg
    23041 ccagggtcgc ggtgtacagc agcgccgccg acaagggggt gcgcagctgg tgggccagcc
    23101 ccgccaccat ctcgcccatg gcagcgagcc ggccggtacg ttcggccgcc aggcgggcgc
    23161 gatgggcctc ggtgacgtca ttgatcagca cgatgccgcc ctcgccggat tccagcggcg
    23221 cctcctgcag ggtcacccgc cgcggtccgt cgccgcatgc cacggcccat tcgcccggcg
    23281 tctcggtggc caccagccgg gacccggaaa aatggctcca gagggtcccc ggcggcaccg
    23341 tggcgaacat ccgctccacc acgcgattga cctgcagcac cgcgccggcg cgatcgagca
    23401 ccaccacccc cgcgggcagc gcctccatca gcacgctgaa gcgttcggtc aactggccga
    23461 cctgggcctg caacccggcg taggcgccgg tcagggcctc ggaagcctcg ctgaactgcc
    23521 ggaaggcctc ggccagctgg cgcgcgtcga gacggtcttc ggcgacggtc gggatcatgg
    23581 gcacacggaa acgaatttca ccggctcgca gcttatgccc gcccttgtca cgcctaggcg
    23641 gcaaataggc ggcaaaaagc gtcgctaatt ccgccgtcgg cggcaggacg gggcgcccag
    23701 aatgcgtgca tccgtaaaag ggagactgtt cccatggcag ccgaagacgc cgccgccccg
    23761 cccgttccgc ccccatcgcc cttcacccag gtgctggaga acatccgcgc cctgagccag
    23821 cgtcagaaga tcgctgccgg cgccgccctg gctgccgcca tcgccctggt ggtgggcgtg
    23881 ctgctgtgga gccgccagcc ggattacgcg gtgctcttct ccaatctggc cgagaaggac
    23941 ggcggcgccg tcgtcgccag cctgcagcag cagaacatcc cctacaagtt ctccgacaac
    24001 ggcaacgcca tcctcgtccc gagcacccag gtccacgacc tgcgcctgcg cctcgccgcc
    24061 cagggcattc ccaagggcgg cctggtgggc ttcgaactga tggagaccca gaagctgggc
    24121 ctgtcccagt tccacgagca ggtcaatttc cagcgggcac tggagggtga gctgtcccgc
    24181 accatctcgt cgatcgccgc ggtggccggc gcccgcgtgc atctggcgat tcccaagcag
    24241 accgccttcc tgcgggacga gcagaagccc accgcctcgg tgatggtcaa tctgcaccag
    24301 tcccgcagcc tcgacccctc ccaggtggcg ggcatcgtgc atctggtggc ctccagcgtg
    24361 cccaacctca ccaacgacaa cgtgagcgtg atcgaccagg acggcaacct gctcaccaaa
    24421 aagccggacc ccatgcgcgc cggtctcgac gccacccaga tcaagtacac ccgcgagctg
    24481 gaagagggct acatccagcg catcaacgac atcctcacgc cgctgttcgg caagggcaac
    24541 ttccgcgccc aggtcaccgc cgacgtggat ttcaacgtca ccgagcagac cgccgagacc
    24601 taccgcccca accccagccc cgaacaggcc atccgcagcc agcagagcag cgagagccag
    24661 acccgcgacg ccggcgccca gggcgtgccc ggcgctctca ccaaccagcc gccggtgccc
    24721 gccaccgccc ccatcaccac gccggccgtg ccgggcacac cgggcgccaa tggccagctc
    24781 cccctcagca cgagcaaaaa cgccaccatc aattacgagc tggacaagac gatccagcac
    24841 acccgcaagg ccctcggcca gattcgccgc ctgtcggtgg cggtggccct caaccagaag
    24901 gaagagaagg acaagaacgg caacgccaag ccggtccccc tcaccgagga ggaacgcaag
    24961 cgcgtcgagg atctggtgcg cgacgccgtc ggctacagca aggaccgggg cgacaccctc
    25021 agcgtggcga gcagcccctt cgtcgcctcg gaaaaggatc tgccggaaac cccattgtgg
    25081 aaggacccgg acaacatcgc cctgttcaag gagctgctca agtacctggt cattgccggg
    25141 gtcatcgcct ttgtcgcctt cggcgtgatc cgtccgctga tgaagcaggt catgccgccg
    25201 cccccggaga aggccgagga agaggcgtcc gccgaatccg cgggcgaagc cggcgaggaa
    25261 ggcggaggcg aaccgggcga agagggcgag gaaggcgccg aggtggaact ctcccccgaa
    25321 gccgcggccc gcctcgccta cgaggaaaag ctcgcccgcg tgcgcgatct ggcccgcagc
    25381 aacccgcgcg tggtcgccaa cctgatcaag gaatggatgg gcgtcaatga gcagcgatga
    25441 aggcctcgag aagagcgccc tgttactgat gaccctcggc gccgacgagg ccgccgaggt
    25501 cctcaaactg ctgggcccgc gggaagtgca gaagctcggc gcggccatgg ccgcgatgcc
    25561 cgcccagtca cgggagcgca tggaggccat cctcgacgag gtggaggccc tggccgaaaa
    25621 aggctcgccc atcgaggccg accatgagca catcaaggcc atgctcacca aggccctcgg
    25681 cgacgagcgc gcgtcccacc tcatcagccg cgtgctacag ggctccgaca ccgccggcat
    25741 cgagagcctc aagtggatgg acgccccgac ggcggccgat ctcatcaaga acgaacaccc
    25801 gcagatcatc gccacgatcc tggtgcacct ggaattcgac caggccggcg agatcctcaa
    25861 gcacttcacc gaccgcctgc gcaacgacgt gctgctgcgc atcgccaccc tggacggcgt
    25921 ccagccggtg gccctgcggg agctcaacga agccctcaca cgcatgctcg ccggcgccag
    25981 ctcggtcaag aagaccgcca tgggcggggt gcgccacgcc gccgacatcc tcaacttcgt
    26041 cggctctgcg gccgagaccg caatcctcga caacgtgcgc gaatacgatc cggacctggc
    26101 ccagaagatc ctcgacgaga tgttcgtctt cgagaacctg cttgatctcg acgatcgggg
    26161 catccagacc ctgctgcggg aagtgcagag cgactccctg gtcatcgccc tcaagggcgc
    26221 accgcccgag atgcgcgaaa aggtcttcaa gaacatgtcc cagcgcgccg ccgagatgct
    26281 caaggaggat ctggaatccc gcggccccgt gcgtttgtcg gaagtggagg cggagcagaa
    26341 agagatcctc aagaccgccc gccgcctcgc cgaggaaggc cagatcgccc tgtccagcaa
    26401 ggggggcgac gatgccttcg tgtaagcccc ggggccgacc atggccctga accgccacca
    26461 agcgagcggg gcctaccgtc gctgggactt cccctccttc gacgcaccgc cgcccgaagc
    26521 gttggcgccg ccccccggcg agccgccccc cgacgcagcc gtgacggcca ccgccccggc
    26581 gcccgatccg tccgaaccgc caccggtcga agcttccccc gccgaggcgg cgctgcctct
    26641 gcccgaaccg gacatccagc tccccaccgc cgaagacatc gagcgcatcc acgaggaggc
    26701 gcgcaaggaa ggctatgccg ccggttacga ggaagccacc gcccgcggtc gcatggaggc
    26761 catgcgtctg cacgggctcg tggaaggcct cgaagcggcc atgagccgcc tcgacgcgga
    26821 cgtggccgag gaggtcctcg ccctgtccct ggaggtcgcc cgcctgatgg tccgccagac
    26881 cctcgcgacc cgcccggaga gcgtcgtcga cctgatccgc gaggccctcg tccagctgcc
    26941 ccaggcccac gcccagatcc atctcaaccc ggaagacctg ggcatggtgc gtgactacct
    27001 gggcgaactc ctcacccacg ccggccaccg cctcgtggaa gatgccggcg tcgcccgcgg
    27061 cggcgcacgc atcgagggcg gcggcagcca ggtggacgcc accgtcccga cccgctggcg
    27121 gcgcatcatg gacaacctgg gccgcaacat cccctgggag ggcgaaggct gatggaccac
    27181 ggcctcgaaa cctggcgggc cttcctccac gacagccgca agctggtcaa cgccacctcg
    27241 ccgctgcaac tctcgggacg cctcacccgc atcaacggcc tggtcatgga agcgtccggc
    27301 ctcaagctgc ccctcggctc cggctgccgc atcgtggtgc cgggcggcgg cacggtggag
    27361 gccgaagtgg tgggcttcca cggcgagaag atcttcctga tgcccaccga tgacgtcttc
    27421 ggcctcgccc ccggcgccca ggtcctgccg gtggaaccga cccagcccca gctcatcgcc
    27481 ggcgagcgca tcgaaccgcg ccgccgggcc tccgaccggg ccaagcacct gcccatgggc
    27541 gacgccctcc tcggccgcgt ggtagacggc gccggacggc ccctcgacgg cctcggcccg
    27601 ctggacgtca agcacacccg ctccctgcag gcacggccgg tcaatccgct gcagcgcgcg
    27661 cccatctccc gctccctgga cgtgggcatc cgcgccatca acgcgctgct caccgtcggc
    27721 cgcggccagc gcatggggct cttcgccggc tccggcgtgg gcaaatcagt gctcatcggc
    27781 atgatggccc gctacaccgc cgccgaggtc atcgtcgtcg gcctgatcgg cgagcggggc
    27841 cgcgaggtca aggaattcat cgagcacaac ctgggaccgg aagggctcgc ccgctcggtc
    27901 gtcatcgccg ctccagccga caccagcccg ctgatgcgcc tgcagggcgc ctcctacgcc
    27961 accaccgtcg cagaatactt ccgcgaccag ggcaagcagg tgctgctgat catggactcc
    28021 ctcacccgct acgccatggc ccagcgggag atcgccctgg ccatcggcga accgccggtc
    28081 acccgcggct acccgccttc ggtcttcgcg cgcctgccgg ccctggtgga gcgggccggc
    28141 aatggccccg aagggggcgg ctcgatcact gccttctaca ccgtgctggc cgaaggcgac
    28201 gaccagcagg atcccatcgc cgactccgcg cgggccatcc tggacggcca catcgtgctg
    28261 tcccgcaatc tggccgacca gggccattac ccggccatcg acatcgaaca gtccatctcg
    28321 cgggcgatgc acaacctggt caagcccgcc cactttgacg tggtgcggcg cttcaagcag
    28381 ctcttctccc gttaccagcg ctcccgcgac ctgatcgctg tgggcgccta ccaggccggc
    28441 tccgacccca tcctcgacca ggccgtggcc tactacccgc gcctggaagc cttcctgcag
    28501 caacgcatcg aggagagcga gccctacgag agcgccgtcg aaaaactcga agccctgttc
    28561 gccggcacgt cctgaaccgc ccaatgcctc cgaccctcaa gattcccgcc ccccgcccga
    28621 tagcacccca ggaacgtgaa gatgactgag ccgttccccc tcaagcctct gctggatctg
    28681 gccaacaccc gcatggacga ggctgcgcgc cgcctgggcg aactgattgc cagcgagcac
    28741 gcggtcgaag agaagctcgc cctgctcaag gactatcgcg acgaatacca ggcgcgcttc
    28801 atggaagccg cccgcaacgg catcggcccg gacgcctggc gcaactacag cgccttcatc
    28861 gcccggctcg acgacgcgat cgcccagcag cagaagctgg cctccgactc ccggcggcgg
    28921 accgaacagg gtcagcaggc atggcgggac gagcgcaaca aggtcaaggc ctttgacacc
    28981 ttgtcccagc gccatcacac cgcccagctc cgcaaggaac tgaaacagga gcaacggctc
    29041 accgacgaac acgccgccaa gcatttccgc gaccgggacg ccgacgacga cggcgagccc
    29101 tgaacgcccc gccgggaccg gcatggcatg cggattgcag tggatcgcct gtccgcatcc
    29161 tcaccaggaa cccgccatgc ctgccacgag cctgaacccc atcgcgaccg tcgccccgcc
    29221 cgccccaagc ggcaacgcct ccgcctcccg tagcggcgac gcgcgcacgc cggaagcatc
    29281 ggcatccgcc gacggcgatt tcgggtctgc cctcaagcag caagtggatc gccaggcgga
    29341 cgatcggcca cgcagcgccg acgaggcggg cagcgcagga tcgaagaccc gcgacgacac
    29401 agccgccgca caggcgcccg cggcaccgcc cggcgaagcg ccgccgtccg cggaagcgcc
    29461 ccagcgcctc ggactcgccc tcttcctcgg ccaggcgaca ggccgaagcg aggcagacgg
    29521 ccgaccggct gcacgcaccg acgacgacgc accggccacc gagtcgccgt cagcggccct
    29581 cgccggcctc ctcgcgcccc cgattgatcc gcgcccgacg acgccggccg aaagcgctcc
    29641 ggccgacacc accggcaaat cgccggcaaa aactgccgca ggcttgccgg aattcgccgc
    29701 cgcgttccga accacaccga ccgatgcggg cagcgccgat gccccacgcc tgcccgccgg
    29761 caccgacgcc gcccccgcca acagcttcgc cggcctgcac gccgccgccc tggcccagat
    29821 ccgcggcgaa gccccgacac ccgccagcaa cgccctgagc ctgccggtgc acacccctgc
    29881 cacccatgcc ggctggccgg aagaggtcag caaccgggtg agctggatgg tcggccagca
    29941 cgaaagccag gccgaactca ccctcacgcc gccgcagctc ggcaaggtcg aagtgaccgt
    30001 ctccgtatcg ggcgaccaga ccagcgccca gttcgtggcc gccacgccgg ccgcccgcga
    30061 tctcatcgag caggccctgc cgcgcctgcg cgagctgctc ggcgatgccg gcatcgccct
    30121 cggccagacc tccgtgggca cgtccggcca gtcgggcaac ggcgagggtg gtgacggccc
    30181 acggcgcggc actgccggcc gcggcacccg cggcgtagat ggcatcggca gcgtccaggg
    30241 cggttcggcc ggtccctggc tgcgccgcgg cgaaggattg gtggacacgt tcgcctgaaa
    30301 ggcacaaatg gccggcgctt ccgccgctat tccgggcttt ccgggcgtcc gtgcaggatg
    30361 acaatcagac actaaaagga gtgatccagc atgtcgaaag caccggccaa ggccgaagcc
    30421 gccgaaggcg aagcccccgc caagggcggc ggcaagaaaa aactcatcat catcgtcgcg
    30481 gtggtcctga tcctggccat cgccggcggc gccgcgttct tcctgctcgg caagaagaag
    30541 cacgcggagg gcgaggaaga ggaagagccc gcccaccacg agccgcccaa ggtcaaggtg
    30601 gatccgtcca agccgccggt cttcgtgcag ctggaccaat tcaccgtcaa tctcgcgccg
    30661 gaagagggcg aacacttcct gcaggccacc atggtgctgc gcgtcgctga cgccaaggtg
    30721 ggcgactccc tcaaggccta catgcccgaa ctcaagcacc gcatcctgat gctgctatcg
    30781 tccaaacgac cttccgagct cgccaatgcc gagggccgcg aggaactggc ggacgaggtc
    30841 aaggacgagg ccaacgacgt gctcggctac gcccccaacc ccaagaagaa ggccaaacgc
    30901 cgccccgagg acgacggtcc ggtactggcg gtgctcttca accagttcat cgtccagtaa
    30961 ggaagcgcgg ccgtgtccca ggactttctc tcccaggaag aggtggatgc cctgctcaag
    31021 ggcgtcaccg gggagaccga cgagccggac agcggcgaag agagccagga cggcgtccgc
    31081 gactacaacc tggcgacgca ggagcgcatc gtccgtgggc gcatgcccac gatggagctc
    31141 atcaacgagc gcttcgcccg gtacctgcgc atcggcctgt tcaactacat gcaccgcaat
    31201 gtggagatct cggtcggccc gatcaaggtg cagaaattca gcgaattcgt gcgcaatctg
    31261 gtggtgccca ccaacctcaa cctgttcatc gccaagcccc tgcgcggcaa cgcgctggtg
    31321 gtgctggacc cgaacctggt gttcctggtg gtggacaacc tcttcggcgg cgacggacgc
    31381 ttccataccc gcgtcgaagg ccgcgacttc acgcccaccg aacagcgcat catccagggc
    31441 ctgctgaagg tcaccttcaa cgaatacgaa aaggcctggt cccccgttca gaagctggac
    31501 ttcgagtacg tccgctcgga gatgaacccc cagttcgcca acatcgccac ccccagcgaa
    31561 atcgtcgtgg cggcgtcctt cacgctggag ttcggcgggt cccaggccga aatgcacttc
    31621 tgcttcccgt actcgatgct ggagcccatc cgcgagacgc tgtactcgac catgcagagc
    31681 gaccacatca cccaggacaa ccgctgggtc gggatgatgg gccgccagct gcagacggcg
    31741 gaggtggaac tcgtcgcccg cctgggcaca gcccacgtca ccctgcggga catcgtcggc
    31801 atgaagcccg gcgacgtgat cccgctcaac atccccgaaa ccatccaggc cgaggtggac
    31861 ggcattccca tcttcgaatg ccgctatggc atccagcgca gccagtacgc cctgaaggtc
    31921 gagcgcttcc tcgcccagga cgaagacagc ccgcccgcca aggctcagga gtaagccatg
    31981 tcagacaccg aaaccgacgc ccccgacgac aaccaggtca gcgatgacga ctgggccgcg
    32041 gccatgcagg agcaggccgc ctccgaaggg ctcaccgatc cggcgaccgg tgtggacgaa
    32101 tcgccctatc aggccaaacc tgccagccag ctcttcccgg atttcggtca ggccggcacc
    32161 aagagcggca ccctgaacga cttcgacatg atcctggaca tcccggtgca gctcaccgtg
    32221 gaactgggac gcaccaagat cgccattcgc aacctcctcc agctggccca tggctcggtg
    32281 gtggaactgg acggcctggc cggcgagccc atggacgtgc tggtcaatgg caccctgatc
    32341 gcccagggtg aagtggtggt ggtgaatgac aagttcggga tccgcctgac ggacatcatc
    32401 acgcctgccg aacgcatccg caaactgcgc agctgaacgc tgcccgcgca cgtccgcccc
    32461 cgcggaagtg cgccctcccc tgttccgatc gcgcttgggc gcccttcagg aaagcggccg
    32521 accggccgcg ccgcccagca ccgagcgcac cggacgacga tccggcgcgt tggcccgatg
    32581 caggagccag cgctcgccga ggatctgcag cgcccgctgg cgcttttcct catagtccgc
    32641 ctgggcggac accggcaccg cctccggcac gggtttgacc accggttgaa cctcacgacg
    32701 ccagagcaac atgatcgaac ctcctcgtca tacctccggc tttgagtaac ggccgcgacg
    32761 gcagttcatt gaagcgcagg caatacatga aaaagtgccg gttttgcgtg cctgttcctc
    32821 ccatggcccg tcgcccgggc gtcctatgct gtgattctcc ccgccagaag tccgtccatg
    32881 cgccgcgccc tcgtccctgc cgtgctcctg tccatcggtc cagtcgccct ggccggtgac
    32941 gccgcacccg acctcaccgg cagcgtggtg cagacgctcc tcgccctcgg tgcggtcatt
    33001 ggcctgctct tcggcaccct gcacctgctg cgccgcagcg gcgtggcccg cgccggcggc
    33061 gccgggctgc tccaggtccg tgccgccacc gcagtcggcc cgcgcgagcg ggtcgtgctg
    33121 gtggaggtgg ccggcaagct gctggtcctg ggtgtcgcac caggacgtgt caccccgctc
    33181 cacaccctgg acgccaccga tctgcccgcc gtgccgtccc gcccggccgg cgagcgggac
    33241 ttccagagct ggctccggca gactctggag cgccgcgccc catgaccgcc gcccgcctgc
    33301 gtcgcctgac gcccctgctc ctgctgctgc ccctgccggc cctcgcccag gccctgcccg
    33361 ccctcaccag cggccccgct cccggcggcg gcacccagta cagcctgccg gtgcagaccc
    33421 tgctgctgct caccgccctg agcttcctgc cggcgatgtt gctgatgatg accagcttca
    33481 cgcgcatcat catcgtcatg tccctgatgc gccacgccct cggcacccag acctcgccgc
    33541 ccaatcaggt gatcgtcggt ctgtccctgt tcctcacctt cttcatcatg gcgccggtgg
    33601 gtgaccgcat ttacaccgat gcctgggtgc cccttcagga aaaccgaatc agcttcgacc
    33661 aggccgtcga gcgggccagc gtaccagtca agggcttcat gctgcgtcag gtgcgcgaac
    33721 ccgatctgaa cctgttctcc aagctcgcca atacccccaa ggtggacaag gccgaagact
    33781 tgcccctgcg cgtggtgatc cccgccttcg tcacctccga gctgaagacc gccttccaga
    33841 tcggcttcat catcttcatt cccttcctca tcatcgacat ggtggtggcc tcggtcctca
    33901 tgtccatggg catgatgatg atgagcccgg ccatcgtcgc cctgcccttc aagatcatgc
    33961 tcttcgtgct ggtggacggc tggaacctgc tggtcgcctc gctggtgcag agcttcggct
    34021 gaccccttcc acggacggac gctgccatga cccccaccac cgtcatcgac ctgggccgcc
    34081 aggcgctgga gctcaccctg ctggtgtcgg cgcccctttt catcgccgcg ctggccaccg
    34141 gcctcgtggt ctccatcttc caggccgcca cccagatcaa cgaacagacc ctgtccttcg
    34201 tgcccaaact ggtggccacc ttcatcaccc tggtgctggc cggcccatgg atgatcaccc
    34261 tcctcaccga cttcatccgc cggctcttcg aaagcatccc cgccatgatc ggctgaccgt
    34321 gctcaccgtc accgccgccc agctggacgc ctggctggcc ctcttcgcct accccctggc
    34381 gcgcatcctg gcgatggtga gcagcgcccc ggtgttcggc aacacagccc tgccgcgtcg
    34441 gatacgcctg gtggcgggcc tcgccctggc gctcggcatc gtaccggccc tggcgccgat
    34501 cccggccatt ccgctcgggt cgtggagcgg cctgctgaca atcttccagc agaccctggt
    34561 cggcctggcc atgggccttg ccatgcgagt cgtgttcgcc gccctggacg tgctcggcga
    34621 actcaccagc ctgcagatgg gcctgtcctt cgccaccttc ttcgatccgg gctccaacgg
    34681 ccagaccgcc gtcgtgggcg aattcctcac cctgctggcc accctcatct tcctggccct
    34741 caacggccac ctgctgatgg ccgatgtgct ggcgcgcagc tttgaatggc tgccggtcaa
    34801 cgccctgccc ctccatgccg gcggctgggc ggtgctggtg cgcttcggcg ccgtcacctt
    34861 ttcagccggc ctgctgctgg ccctgccggt ggtcactgcg ctcctgatca ccaacatcgc
    34921 cctcggcatc ctcacccgcg ccgcccccca gctcaacctc ttcgccatcg gcttccccat
    34981 caccctgacg gtcggcttcg gcgtcatcat gctcgccctg gagcgcttcg ccccgctgct
    35041 ccagcgcttc tacgacatgg gcttcgacac catagcggcg ctcctcaagg cgctcgcctg
    35101 atccgcgcgg cgacccgcat gttcgccatc tccccctccg ctgcgctggc cctgtccctg
    35161 gccatttcca tgctgctggc cgcgagcctg cgctacgtgg ccgccgtgga gcgtccccag
    35221 actgccctgc gggcgtgggc cgccgccttc gcctgccatg ccgtccacga cgctgccctg
    35281 ctggcccccg cgctcaccca cctgccgctg gcgctcctcg ccccggtcgg tctgatgggg
    35341 atgagtgccg ccttgtggat cggtctgggc cgggtcaccg gcgacggaca ctcgccccgt
    35401 gtcatgctgc tgcccctgct ggccgccctg tgggccctcg tccccacggc gccgggacgg
    35461 tcggaagtcc tgcgcacgct cccggtggat gccgtggcga tcaccttcac cttcgccgca
    35521 gcccgcgtct tctggcgacg cgccacgtac cagccgggca ccggacaccg tctcgccgcc
    35581 gtgggccttg gcggttgggg tctcgtgctc ctcgcgggct ccgcgggcgt gctggcggac
    35641 ggcagcgtgg cccgcgccct ggccgccgcc ctggcccttt ccggcctcgg ctgcgcactg
    35701 ctgatcaatg cgctgtgcct gcagaacgcc gcagaggcgc gcctcaccac ccacctgcgg
    35761 gccgagatgg accgccggat cgccgccgag tccgcgctgg cagaatccca cagccggttc
    35821 acggcgcttg ccgagagctc ggacctgggc ctttttgtgg tgagcccgga cggtcagttt
    35881 caatacacca actcccgcta ccgggccctg agcggcctca ccgacgaaga actgcgcacg
    35941 cgtccggaca cccagcgcat ggacggcgaa gatgccgccc gcgtctcggc cgcctgggcc
    36001 gaggcacgcg ccctgcgctg ccagacccat tacgagcggc gcgtcgtgca gagcggggcg
    36061 acggaaggct ggctgtcggt gcacgtggcc cccatcaccg ccggcgacag cctccaaggc
    36121 ttcgtcgcca cggtggagga catcacggcc cgcaagcagg ccgaggccgc cttgcgggac
    36181 agcgaggaac gcctctccca ggtcttccgg ctgctgcccg atgcggtcat gctcagcgat
    36241 ccggacagcg gacgcatcga cgacgtcaat caggggtgga cagacacgct gggccacccc
    36301 cgtgaagcca ccctcgggcg cacggccgag gccctcggct tatggtgcaa tccgtccgac
    36361 gcccggactg tcctcgacac cctgcgtgcc ggacatgacg tctccgacca gccggcgacc
    36421 ctgtgccacc gggacggtcg tgtgctggaa accctgctgc gcgcccgcct gatccgcgtc
    36481 cgcggacgcg acggcctcct caccgtcatc cgcgacgtca ccgagccgcg gcgcctggaa
    36541 cgcatcggcc gggccgccgc ggacgccttg cgcgtgtccg aggccatgtt ctccgcggcc
    36601 ttccgggcca gtccggacta cctcaccatc agccgtctgg acggccacct gctggaggtc
    36661 aatccgacct tcgagcggat gaccggctgg cgccgcgacg aggccatcgg cgcccaggcc
    36721 gtcgaactgg gtctgtgggc tttcccggaa gagcgcgagg ccatgctcca cgagctcagc
    36781 gtgcacggtg ccgtgaccga tttcccgtgc acgctcggca cccgctggaa gacccggcgg
    36841 acgtgtctgc tgaatgtctc catggtcacc ctggagaatg aaccctgcct ggtgggcatg
    36901 atccacgaca tcacggagca gcaggcggcc gagaaggcct tgcgggacag cgaacgcaag
    36961 ttcgccacga tcttcaacct ggcccccttc gccctctccc tcacgcgggt cggcgatcgc
    37021 acccacgtgg atgccaatcc ggcctgggaa cgcctcttcg gcatcgagcg gaacaccgtg
    37081 ctcggacgca cgtccgggga attcggctgc tggaccgatc ctcgcgccta cgacgatctg
    37141 tacgcccggg tagtctccag cgacgtgctc gcgcagcagg aaatccgcat ccggccctgc
    37201 aaccgggacg acgtcgccac ctgcctcatc tccgggcagc gcctgcgcat ccgcgacgaa
    37261 gagtgcgccc tgtgggcgat ggtggacatc agcgaactgc gccgtgccca ggctcgcatc
    37321 gaagagctca accagggcct ggaactgcgc gtcgtggaac ggaccaccga actctccaac
    37381 gccctcgaag ccctgcgccg gacccaggaa gacctggtgc gcagcgagaa gctcgccgcc
    37441 ctcggctcca tggtggcggg catcgcccat gagctcaaca ccccccttgg caacagcgtc
    37501 acggtcacct cgaccctcgc gacccggacg gacgagctga cccaccgcaa ggcggccggc
    37561 cccctgcgcc gcagcgatct ggaaagctac ctggacagcg tccgcaccgc ctccgacctg
    37621 ctgctgcgca gcctcagccg ggcccgcgac ctggtcagca gcttcaagca ggtggcggtg
    37681 gaccagacca gcgaacaacg ccgccgcttc gaactcggcg aggtcctgcg cgaagtggtc
    37741 acgaccctcg gccccatgtt caagggcacg ccctacaccc tccggatcga gcccgccccg
    37801 gccctggaga tggacagcta ccccggcccc ctgggccagg tgatcaccaa cctggtcagt
    37861 aatgccctcg tccacgcctt cgccggacgc gaccacggca ccatcaccct gacccccgcc
    37921 gccgacggcc cggcgggcgt cacactgtgc ttccgggacg acggccacgg catcgacccg
    37981 gcccacctgg ggcgcatctt cgacccgttc ttcaccacgc gcctgggcac gggcggcagc
    38041 ggtctcggcc tgcacatcgt ctacaacatg gtcacgcggg tgctgggagg ccgcctcgag
    38101 gtcagcagcc agctgggcgt cgggaccacc ttcaccctcg cgatgccccg caccgccccg
    38161 gccaccgggg gcacagaaaa tcccatgagc atttctgcaa aacctgccgg acgttcattc
    38221 aatttcgagg acaacgggcc gataatctga ggactctggc cggattcctc tcccggtccc
    38281 gcttcctgga gcaagcatgg ccatcgccat cgtcgacgac acccccctga atctcacact
    38341 gatccaggcc ctcgtccgca aaatcgcccc gcccgacacc gagatccgct gcttcacctc
    38401 gcccctcgaa ggcctgcagt ggtgcagcga gcacgaaccc gatctggtca tcgtcgacta
    38461 catgatgccg gacctcaacg gcatcgaatt catcacctgc ctgcgcaagg tgcacccggc
    38521 cgaagccgtg cccatcctga tggtcaccgc cgcccacgaa aaggagatcc gctaccagag
    38581 cctcaccgcc ggcgccagcg acttcctcac caagcccatc gaccgctacg agttcgaccc
    38641 ccgcgtgcgc accatgctcg ccctgcgcct gtcccacctg cgcatgcgcc actacaacga
    38701 cgacctgcgc gaagccgtgc gccgcgccac cgccgacatc gtggcgcggg aacgcgagac
    38761 cgtcacccgc ctcgcccgcg cggccgaatt ccgcgacccg gagaccggcg cccacatcct
    38821 gcgcatggcc cactactcgc ggctgatcgc cgagcagctc gggctcggcg ccgaattctg
    38881 cgaacgcctg ctcagcgccg cccccatgca cgacgtgggc aaggtgggca cacccgacca
    38941 catcctcctc aaacccggcc gactcactga cgaggagatg gccatcatgc gccagcacgc
    39001 cagcatcggc cacgacatcc tcaagggctc gtcctcaccg atgatccagc tggccgccga
    39061 gatcgccctc agccaccacg aaaagttcga cggcagcggc tatccgggcg gactgacggg
    39121 cgaggcgatt cccctgtcgg ggcgcatcgt cgccgtcgcc gacgtcttcg acgccctcac
    39181 ctccgagcgg ccctacaagc ccgcctgggc gctcgaccgg gccgtgcagt ttctcaagga
    39241 tgggcgcggc gcccatttcg acccggcctg catcgacgcc ttcctgctgc gctgggacga
    39301 ggtgctggcc atccgggacc gttaccgcga cgaatgaaag cgctgttcga ccgtctcgcc
    39361 agcctcctcg ggcttgactc cctgcgccag cagctcaccc tgctgttcac cgtcctgctc
    39421 gggctcgcca tcggcatcta cgccctgtac accgccgtcg aacaggccgc ttacgtggag
    39481 cgcctggaac gtcagcgcgc cgaagagagc gcccggcacc tggcagacga tctcgcgccc
    39541 cacctgaccg ccggcgatcg gaccggtctg acgcaccatc ttgaagcgct cgcccgccag
    39601 cgcgacatgc tccacctcac cgtcaccgac cttcacggcc tgccggttgc cggcgtgagc
    39661 gtgaccgagg gcgcgccccg cttcgatgca gccgccgacc ggcccctcac cctgcccgcc
    39721 gacacgcagc cggccacgcg ccagggcgag accgacgcca tcgttgcctg ggccgagatc
    39781 cgcgatgatg tcccccgcgg ctggctgcgc ctcgagatcg ccccgaccgc cgcccgcgaa
    39841 gccctggcgc acatcgtcga ggacagcctc atcaccggtc tgctgaccgt cacactcggc
    39901 gccgtcatca tccggcgctt cctgcgcggc cccctgcaca gcctcgagcg ggccaccgcc
    39961 ttcgccgaag gcctggagac cggcgaaggc gcggtcctca acgagcccag ccgccttgag
    40021 gaaatccgca agctcgtgga cgccctcaac tggacctcca tccgcttcta cgacggccag
    40081 tccgccctgg ccgacagcga atcccgcaat cgcgccatca ccgaagccgc cctggactgc
    40141 atcgtcaccg tggacgatgg cggacgcatc atcgaattca atccggccgc cgagcgcagc
    40201 ttcggtgtct cccgcgcgga cgccctgcac cagtacgtgg gcgacctcat cttctccgac
    40261 gagcggcgcg atgaacagga gcggatcctg caacaggtcc tcgatcccga acgtcgggac
    40321 gaagccggtc agcgcttcga aaccctcgtc acccgcgccg ggggccagac ctttccggtg
    40381 gacgtggcca tcgtgccggc catcagctcg ggccgccgcc tgttcaccgc ctacctgcgc
    40441 gacatcagcg accagaagcg cgccgaggag gccatgcgca tggccatgga agccgcggag
    40501 gccaacagcc gcctcaagag cgacttcctc gccaacatga gtcacgagat ccgcaccccc
    40561 atgaacgcca tcctggggat gaccgacctc gccctcgaca ccgatctgga cgccgaacaa
    40621 cgggagtacc tgacgctcgt gaaaagctcc gcccaatccc tgctcaacat catcaatgac
    40681 atcctcgact tctcgaagat cgaagccggc aagctggact tcgagcacat cgacttcagc
    40741 ctgcgcgact gcatctccct ggccacccgc accctgcagc agcgcgccgc cgaaaaagac
    40801 ctccagctcg tcacccagat cgaaccgcag acccccgaca ccctgcgcgg cgacccccat
    40861 cgcctgcgcc aggtgctcat caacctcatc tccaacggca tcaagttcac gccccacggc
    40921 gaggtccgca tccgcgtgca tcccacaccg ggcaacaagg acgcgggccg catcgagctg
    40981 acgttcagcg tgcaggacac cgggatcggg atcccgcggg acaagcaggg cctcatcttc
    41041 gacgccttct cccaggccga cacctccacc acgcgccgct atggcggcac cgggctgggg
    41101 cttgccatca gcgcccagct tgtcaagggc atgggcggcg gcatccgtgt cacgagcgag
    41161 ccgggccagg gcagcacctt cgagttcaac gccgtgttcg agcctggcca gtcggtggta
    41221 cccgtggaaa ccgacaccga acttgaaggc ctacccgtgc tggtcgtggt gaagagcgca
    41281 tccgaccggg gcatcctgtc ggagctcctc acccactggc gcatgtcgcc gctgatggtc
    41341 agtgacgcca gcgccgccca ccgggaactc ctcgccgccg ccaagcgggg cagcccctac
    41401 cgcgtcgccc tgcttggccg cgatctgccc gacatggatg gcttcgacct ggcgagtgcc
    41461 ctttccgccg cctcgccccg ccccctgtcc gtgatcctgc tggccgaacg ggggcgtcgc
    41521 ggtgacggcg cccgctgccg cgaaatgggc atcgccgcct atctgccgat gcccatccag
    41581 gcctcggacc tgctcaacgc gatcctcctg tcctgcgacg tgggcggcga agccgacggc
    41641 gaccgcccgc tgatcacccg ccacagcctg cgcgaacaga agcgcgtcct caacatcctg
    41701 ctggccgagg acaaccccat caaccagacc ctcgccctgc gcctgctggc caagctcggc
    41761 caccaggtca cggtggccaa caacggcgcc gaggccctcg aactgcacgc ccgccagcgt
    41821 ttcgacatca tcctgatgga cgtgcagatg ccggtcatgg gcggcttcga gaccaccgcc
    41881 gagatccgcc gccgcgaagc ggccggcacc ccccacacgc ccatcgtggc gatgaccgcc
    41941 catgccatga agggcgaccg ggagcgttgc ctcgacgccg gcatggacgg ctacctgtcc
    42001 aagcccatca acgccccgga cctggtggaa gccctcatcc atcacgccga ccagagcgac
    42061 ccgccacctc agcccgtggc ccacgagacc gtcgccctcg ggccggtgtt cgaccgggcg
    42121 gcggtgctgg ccaatctggg agacgatgag gaattactca cgcagctcat cgccatgtac
    42181 ctgcacgacg agccacgcat ggtgggcgaa ctggaaaacg ccatcgccca tcaggacgcg
    42241 gaagccgtca atgccgccgc ccacgcgctg aagggcgccg tcgccaactt tttcgcctgc
    42301 cgcgcccagg ccagggccat gcagctggaa cagctcgggc gccacaagca tctcagcgac
    42361 gcacccgccg tcctggccga gctgaagcgg gagctggcag ccctgcacga ggccttccgc
    42421 agcgtcgccc cggcggacgc ctgaccgtac ggtgcctgcc ggacggggat ttcgcccggc
    42481 cccggcccct cagccccggt tcttggcctg gatcagttcc accttgtagc cgtccggatc
    42541 ctcgacgaag gcgatcaccg tgctgccatg cttcatcggc cccgcctcgc gggtcacctt
    42601 gccgccgcgc gccttgatct cggcgcaggc ggcataggcg tccggcacct ccagggcgat
    42661 gtggccatag gccgtgccca gcgcatagga cgtcacgccc cagttgtagg tcagctcgat
    42721 gaccgcgcca tccgcttcgt cctggaagcc cagaaaggcc aaggtgaact gtccgtccgg
    42781 atagtcgctg cggcgcagca ggcgcatgcc gagcacttcg gtgtagaaag cgatggagcg
    42841 ttccaggtcg ccaacgcgga gcatggtgtg gaggatgcgc atggggagtc cttcgatcaa
    42901 agctgcggga gggaggccgc cactgcccgc agacgggcac gggcaaccac gtgttcggga
    42961 tagaccgact cgaccacgga ccagaagcgt ggcgagtggt tcatttccag cagatgggca
    43021 acctcatgcg ccgcgacgta atcgacgagc tcgaggggca ggtggatcag ccgccagtgc
    43081 aggcggatgc cgctgcgccg gctgcagctg ccccagcgcg tgcgcgcgga ggacaacccc
    43141 accgtcggca ccggctggcc gatccgtagg cagaactctg caacccggtg ccggaacacc
    43201 tccagcgccc gctgctgtac gccccgtcgc aacacggccg ccggatcgct gccggcgcgc
    43261 agggcgattt cgaggatgcg cggcgcgtcc gccgtcacgc ccccttcgtg ccagaccagc
    43321 cggttgagtc ctttcgacag gtgtagcgtc caggtttcac cgagcaccgg caacccggcg
    43381 ccctccacca cggcaaacgg gggtgcctcg gcgcgggccg cgtggacctg cactttgtcg
    43441 aggatccagc gggcatggtc gtgcagaaag gcgtccaccg cccgctgggt ggcccgcagc
    43501 gggacgccga cggtgagccc ccgcgcgtcc acccgcaggc cgaaggtacg gcgggcgcta
    43561 cgcttcaagg tgtaggcgat cgccacaccg tccagcagga cctcccgctg cagtccgtcc
    43621 ttccgcggcg acgccggaag gctcactccg cggccggctc caccgcctcg gccgcgcgct
    43681 gacgggtcgg gcgatagtgc cccgggaaga gctggcgcat ctcgccttcg atccaagcct
    43741 gcgccttggc gttgatctgc tcggcattga ggcccttggt gtcgatgacc ggcccgacgc
    43801 tgaccgtcac cacgcccggg cgcttgaaga gggcgttgcg cggccagaac tcgccggcgt
    43861 tgtgggccac cggcaccacg ggcacaccgg tctgggccgc cagccacgag gcgccggcct
    43921 tgtagcgctt cttgctgccc ggcgcgacgc gggtgccttc cgggaagatg gtcacccaga
    43981 agccctcggc gaggcgttcg cgtccctggg cctccacctg ggtcagggcg tccttgccgg
    44041 ccgcgcgatc gatggcgatc atcggcatgc gggcgatgcc ccagccaaag aagggaatgc
    44101 tgtggatctc cttcttcagc acgaagacca tgggcggaaa gatgtcctgc agcaggatcg
    44161 tctcccacgc cgacatgtgc ttggagacga tgatggccgg gccttcccgg ggaatgttct
    44221 cggcgccgac gagacggtag ggaatcccca gcacgtgctt gatgacccag cccatgatgc
    44281 gcggccagcc gcggatcaat ttgtgccgcg tctgcggccc gaagggcgaa ctgagcacgc
    44341 cgatcagggc gtagagcggc gtgaacaccg ccagggccag catgaacagc aggttgcgga
    44401 tcaggaccac cgttgcctcc tcaggccgtg atctgtgcga cgacggccgc caggtcgggg
    44461 aagatctgcg tgccttccgg caggtcggga tcgagccggg tcttgccgcc cttgccggtc
    44521 agaaccaggt aaggcgcgca gccgacggcc acaccggcct gcaggtcgcg caggctgtcg
    44581 cccaccaccg gctggccgcg caggtccacg ttgaagcgct cggcgatctg ccggaacatg
    44641 cccggtttgg gcttgcggca gtcgcaggtg gagtcggcgg catgggggca gaagaagatc
    44701 gcatcgaggc gcccgcccgc ctgggccagc gccttgacca tcttttcgtt gatggcgttg
    44761 agggtgtcca tgtcgaacag gccacgcccc accccggact ggttggaggc gatcaccacg
    44821 cgccagccgg attcattgag gcgggcgatg gcttccagcg aaccgggaat gggcttccat
    44881 tcctcgggcg acttgatgaa ctggtcggag tcctggttga tgacgccgtc ccggtcaagc
    44941 acgatgatct tcatgaatcc ccccgtccgt ctcacgccga caggcgcgag atgtccgcca
    45001 cctggttcat cagcccggcg agctggttga gcagggccag gcggttctgc cgcaccagcg
    45061 gctcttcggc catcaccatt acctcgtcga agaagcggtc caccgccgcc cgcaggccgg
    45121 ccaggacgcg cagcgcgtcc gtgtaggcct cgttctggaa gtgggagcgg gccagcgggg
    45181 ccacatccac gatggcgtgg aacagggcct tctccgcgtc ctcggcgagg agcgccacct
    45241 ccggctcggc aaattcgcca ccggccttcc tgaggatatt gacgatgcgc ttgttggctg
    45301 ccgccagggc ctgggcctcc ggcaactgct gaaaggcggc gacggcctcg agcttgggca
    45361 ccaccagatc gatgcgggtc gggcgcaggg ccagcaccgc gtccacggcg gccggcgcat
    45421 ggccggcgtc acgcagcagg ttgcgcagac gttcgagcat gaagtcgaac aggggcgcat
    45481 ccacctcggc ggtgagcacg ccgggcggga agctcgccac cgcgtcggcg atgagggccg
    45541 ccaggtcgag gggcagcggc gcctccatga ggatacgcag cacgcccagg gcggcacgac
    45601 gcagcccgaa ggggtccttg tcgccagtgg gcacctggcc gatgccgaag aagccgacca
    45661 gggcatcgag cttgtccgcc agggccaggg cccggccgat gttgctgtcg ggcagcgcgt
    45721 caccggcaaa acgcggccgg tagtgctgct cgatggcatc ggcgacaacc gcctgggcgc
    45781 cctcgtggag cgcgtagtag cgccccatga tgccctgcag ctcggggaat tcgccgacca
    45841 tgtcggtgac caggtcggcc ttgcacaggc gcgcggcctg gccggcctgg gcgtcgtcgg
    45901 cgccgagcct gacggcaacg gcccgcgcca ccgcatcgag gcgctgcacc cggtcgccca
    45961 gggagccgag cttgttgtga tagaccaccg aatccagctt gccgacccgc gagctgaggc
    46021 cggccttgcg gtcctgctcg aagaagaagc gggcatccga caggcgcgga cgcaccacgc
    46081 gctggttgcc ggtgacgatg ttggacggat cttccagacg catgttggag acgatcagga
    46141 agcggttgag cagcttgccc tgggcgtcga acagggggaa atacttctgg ttggcgcgca
    46201 tcgtgaggat caggcattcc tgcggcacgg cgaggaattc ggcctcgaac tcgcccacgt
    46261 agaccgtggg gtgctccacc agcgcggtga cctcgtccag caggtcggcg tattgcccga
    46321 ggctggccgc ctggcgcgcc gcctcgcgga cgagctgctg ctcgatgtcg gcgcggcgct
    46381 cggcgaaatc gggcacgacc ttgccttccg ccagcagggt gggctcgtag gcgtcggccg
    46441 tggcgacgac gatctcgccg cggctcatga agcggtggcc ccgcgtgacg cggccagccg
    46501 ccagttccag cacggtgccg ggcaccacgt cggcgccgtg gagcatgagc agcgaatgca
    46561 ccgggcgcac gaaagccacg tcgctgtcgc cccagcgcat caccttgggg atcggcagcg
    46621 ccttgagggc gtcctggacg ataccggcca gtacgtcggc gagccgcgca ccgggcaccg
    46681 tcgcggtgtg gaacagggcc tcggccttgc cgtccaggcg ccgctcgaag gtggccacgg
    46741 ccgacagggg aatacccttc gcctccagct ttttgaccag cgcaggcgtg ggctggccgg
    46801 cggcgtcgag ggccacggac accggcatga tcttttcggt cacgtcgcgg gcggcggcct
    46861 cggccaccac accgctcacg ctgacggcca gccggcgtgg cgtggcaaac cagcggaagg
    46921 cgccctccgc cgcggccagg ccgcgggcgc gcaggccttc gacgacaccc ttggcaaagg
    46981 tctgaccgag gcgcggcaag gccttgggcg gaagctcttc ggtgagcagt tcgacaagca
    47041 gggtcgcgcc ggtcatcacg cggcctcctt cttcagcatc gggaagccga gggcttcacg
    47101 ggaattgaaa taggcctggg ccacttcccg ggccagggcg cggacgcggc cgatgtaggc
    47161 ggcgcgctcg gtgaccgaga tggcgccgcg ggcatccagc aggttgaaag tatgggaaca
    47221 cttcatgacc atctcgaagg ccggcagcac caggggcacg gccatcaggc gcttggcctc
    47281 ggcttcgtat tcgccgaaca ggcggaacag ccattccacg ttggagtgct cgaaattgta
    47341 ggtggactgc tccacctcgt tctggtggta gacgttgccg taggtgaccg gcgtgccgtc
    47401 cgggccgatc gaccagacca ggtcgtagac gttttccacg ccctgcaggt acatggccag
    47461 gcgctccaga ccataggtga tctcgcccag caccggcttg cagctcaggg agccgacctc
    47521 ctggaagtag gtgaactggg tcacctccat gccgtccagc cacacctccc agcccagccc
    47581 ccaggcgccc agggtgggcg actcccagtc gtcctcgacg aagcggatgt cgtgggcgtt
    47641 gggatcgatg cccagcgccc gcaggctgtc gatatacagc tcctggatgt tggccggcga
    47701 gggcttgagg accacctggt actggtaata gtgctgcaga cggttggggt tctcgccgta
    47761 gcggccgtcc ttgggccgcc gggagggctg cacgtaggcg gcgttccagt gttccgggcc
    47821 gatggcgcgc aggaaggtgg cggtgtggaa ggtgccggcc cccacctcga tgtcgtaggg
    47881 ctggagcagg gtacagccgc gttccgccca gaagttggac agccgcagga tgatctgctg
    47941 gaaagtcggt ttatggactg acatggacgc tcggcggcgg tgagccgcgc agaaagggca
    48001 aaacgcggat tttactgggt ttcgccggac tcggcaggct cctgtcccgc cgggaggcgc
    48061 tccgtggaac agatcacata gacccgtctc aaggcagggg tattttctgc cgtaatggca
    48121 gccaatatac aaattgatac atatccaccg ccgcgcccgt tgattcccgt gtacgtccgt
    48181 cctggagagc cctcgtcatg aacctgtccg aactgcgtgt tgcgacgcgg atgcggattc
    48241 tcgtcgcgct gaccctcatc ggtctcgtcg cgctgtgcac cgtcagcctg ttccggctgc
    48301 acgccagcat gatggacgac cgcaagcagc agacccgcta cgtcgtccag ctgggcctcg
    48361 gggtgctcca gcatttccac gccctcgccc agtccggcgc gctctccgat gccgacgccc
    48421 gcaaggcggc catcgaaacc ctgcgtaccc tgcgctacga gggcaccaac tacatcttcg
    48481 tggtgagcac caccagccat tacatcctga tgcctcctcg ccccgagaag gaaggcaccg
    48541 acgccagtgg caccaaggac agtaccggca aggccatcat cccggaactg gtcaaggccg
    48601 ccctggccgg aggaggcttc gtggattacc tcttccccaa gcccggcgga accgatccgc
    48661 agcccaaggt gagctttgcc gcccagttcg gtccctggca atgggtgttg ggcacgggca
    48721 tctacgtgga tgacgtggac gtggaattcc gcgccatcgc ctggaccctg ggcggcatct
    48781 ccgcggtgct gctgatcgtt ctgggcgtgg ccggctgggg cgtcggcatc agcatcatcc
    48841 ggcaactcgg cggcgaaccc cgcgaggcga cggccatcat gcagcaggtg gcggacggcg
    48901 acctctccct gcgcctggag gacagccgcc ccggctccat gctccacgcc ctgtccacca
    48961 tgatcgcgtc gctgcgcacc atgatgggcg agatcaaccg cagcgccgtc cagctcgtca
    49021 gcaacgccga ccacatcagc caggtgtccg gtgaagtggc cgacgccgcc atccgccagt
    49081 ccgacgccac cgcggccatg gccgcggcca tggaggagct cactgtcagt tccagccaca
    49141 tctcgagcag cgcccgtgac accgaggaca actctcagga agccatgcgc ctcgccgccg
    49201 agggtaccca gcgtgtccag caggcctctg gcgcgatccg caagatgtca gagaccgtct
    49261 tgggtgcctc cacgcgcatc cgtgccctgg aaggccgcat ctcccaggtg tcgtccatcg
    49321 ccaacgtgat caaggagatc gccggccaga ccaatctgct cgccctcaac gcggccatcg
    49381 aggcggcccg cgccggcgag cagggccgcg gcttcgccgt ggtggccgac gaagtacgca
    49441 agctggctga acgcacctcc tccgccacca ccgagatcga gcagatgatc gtcggcatcc
    49501 agagcgacac gggcagcgcc gtggaggcga tgaacgccgc cctgcccgag gtggacgagg
    49561 gcgtcagcct cgccgcctcg gccgccgaat ccctggcggc catcgagaac ggcgccaccc
    49621 ggaccctcga acgggtccac gagatcgctg acgccacccg cgaacagagc gctgccagca
    49681 cggccatcgc ccagcgcgtc gaggacatct ccaacatggt ggagaacacc accaagacca
    49741 tccgcggcgc cgccgaggcg gccgtcggac tggaacgcat cgccggcagc ctgcgcgacc
    49801 aggtcagcca cttccgcgtc tagcgggcga tccgcgccgc gcctgggcgg gatgccgatc
    49861 cgcccgtcag gcgcttttcc aagccgaccg ccagcgccag ggcccaggcc gccgccccga
    49921 tctcgagcca cgcggccggc ggcggtggcg ccgatgcgaa gagccggttc atcgccggcc
    49981 accaggtaaa ggccgcctgc aaggcgacca tcgcaccgat cccgagccag aaccacgggt
    50041 tggcgagcac gccgccgcgc cagaagccgc cgcgcaggct gcggcagttg aaaagatagg
    50101 ccacctcgcc ggcaacaaag acgttcacgg cgatcgtgcg cgcctcctcg aggctcgccc
    50161 cgaacccgcg ccagcgcagg aacaggccga aggccgccgc caccagcagc gcgcccacca
    50221 gcacgacgcg gcgcatctgc accgcgtcca gcaccggcgc accgggcggc cgtggcgggc
    50281 ggcccatcac atccggttcg atgggctcga aggccagggt catgcccagc agcacggcgg
    50341 tggtcatgtt gatccacagg atctgcaaag gcgtgatggg cagggtggtg ccggccagca
    50401 ccgcgaagag gatcaccagc ccttcggcga aattggtggg cagggtccac aggatgaatt
    50461 tcaccagatt gtcgaagacc ccgcgtcctg cctccaccgc ggcctccagg gtggcgaagt
    50521 tgtcgtccgt gagcaccatc gctgccgcct ccttggccac ctcggtgcca ccggcgccca
    50581 tcgccacgcc gatgtcggcc gccttgaggg ccggcgcatc gttcaccccg tcgccggtca
    50641 ttgcgaccac ctcgcccccc gcctgcagcg cctgcaccag ccgcagcttc tgggccggct
    50701 ccacacgggc gaacacgttc acccgcatgg ccgcggcctg cagggcgagg gcgtcgagac
    50761 ccgccagatc gcggccggtg agcacggcgt caccgtcggc ggcgatgccg agctgccgcg
    50821 cgacggcgag ggcggtggcg gcatggtcac cggtgatcat cttcaccgtg acaccggccg
    50881 cacggcaggc cgccacggcg cggatcgccg ccggccgcgg gggatcctgc atggcctgca
    50941 agcccaggaa gaccagcccc tgagccggca gctccgcacc cagcgtgccg tccccgcccc
    51001 gcgcaaaggc gagcacccgc aatccgtcag cggccagcgc cgtggcggcc acctccacgg
    51061 cggcgggatc gagcgcctcc gggcatccat ccacgccgat ctgcgccgcg catcgcggca
    51121 gcagcgcctc cagcccgccc ttgacgaaca ccaccggatg gtccgccaca cagaccttca
    51181 cggccatgta aggccgcgcg gcatcgaagg ggatttcgcc aagccgcgga caggcgccgc
    51241 gcagcgcagc gccgtcgagt ccgcccacgt cagcggccac gagcaaggcc acctccgtcg
    51301 ggtcgccgct cgccggcccg ccggcggcat ccgcctgcag gcgcgcgtca ttgcacaaca
    51361 cgcccgccac gagcagttcg ttcaggccgg ccggcagccc ggcgcgtcca ggctcggcgg
    51421 agggttccgg cgtgttttct gcccattcga aggaccgccc gccctgccac aggaccttca
    51481 ccgtcatccg gttctcggtg agggtgccgg tcttgtcgga gcagatcacc gtggtgctgc
    51541 ccagagtctc caccgccggc aggcgccgga cgatggcctt gcggcgggcc atgcgcgcca
    51601 cgccaatcgc cagggtgacg gtcaccgccg cgggcagccc ttcaggaatc gcccccaccg
    51661 ccagcgccac cgccgccatg agcatgtccg caagggcttc accgcgcatc cagccgacca
    51721 cgaagccgaa gaccgccagt ccgaggatca gccacagcag cacccgggaa aatccggcca
    51781 tggcacgggt caggggggtc gccagggcat cggtgacgtc cagcaagtga gcaatgcggc
    51841 cgacttccgt gtccgcgccg gtggccacga ccaccccgca cccctggccc gcggtcacca
    51901 gcgttccggc ataggccatg caggcccgct cggccagcac cccgtccgcc ggcaccgtcg
    51961 ccgtgtcctt ggcgacggcg acggactcgc cggtgagggc cgcctcggcg atctgcaggc
    52021 tgcgggcgtc gaacaggcgc aggtcggccg gcaccttgtc accggcaccg agccggacca
    52081 cgtcgcccgg caccagctcc accgcctcgc acgcgaccat ccggccgtcg cgcagcaccg
    52141 tgaccgcgct cgccacgctg cgggccaggg ctgccagcgc gtcagcggcc ttgccctcct
    52201 ggacgaagcc gatcagggca ttgaccagca ccacgccgaa gatcacgccg gcgtcagccc
    52261 attcgcccag gaggagggcg attgcgccgc tggcgaggag gatgtagatg aggggctgat
    52321 ggaactgctg ggcgaggcgc tgccagggcc cgggccctgg ccggccgggg aggcggttgg
    52381 ggccgtaccg ccgcaggcgc tcggcggcgt cggccgtcgt caggccatgg ggcgaactgc
    52441 gcagggcgtc gaggacgtcc tgcggcgagc gggcatgcca ggcggcggcc cgctcggggg
    52501 ccatcacacc ggcacgcggg gcaggccgga gagggccatg gccagttcct gttcgtcgta
    52561 atcctgatcc accagcttgc cggccaggta attggtgtac gacgccatgt caaagtggcc
    52621 gtggccggac aggctgaaca ggatcgtctc gctgcgccct tccgccttgc agcgcagcgc
    52681 ctcgtcgatg gcggcgcgga tcgcgtgatt ggcctccggc gccgggacga tgccttcgct
    52741 gcgggcgaac atcacaccgg cctcgaagca ggcgttctgg tgatagttgc gcgcctcaac
    52801 gaaacccagc tccttcacat ggctcaccag cggcgccatg ccgtggtaac gcaggccgcc
    52861 ggcgtgaaag ccggggggcg tgaattcgct gcccagggtg tgcatcttga ccagcggcgt
    52921 gaggtgcgcg gtgtcgccga agtcgtaggc gtactggccg cggctgaggg acggacaggc
    52981 cgagggctcc accgccagga tgcggcgccg gcgggcgttc gccccgcccc gcagctggag
    53041 gccgatgaag gggaaggcga tgccggcgaa attggaaccg ccgccagtgc agccgatcac
    53101 cacatccgga tcgtcacccg ccatctcgag ctgggcgatg gcctcctgac cgatgatggt
    53161 ctggtgcagc agcacatggt tgagcacgga gccgagcgcg tacttggtgt cgtcgcgctt
    53221 cgccgccact tccaccgcct cggagatggc gatgcccagg gaacccagat ggtccggcgt
    53281 cttcgccagg acggcgcggc cgctctcggt ctccagcgag ggcgaggcga tgcagcgggc
    53341 gccccaggtt tccatcaggg cgcggcggta gggcttctgg tcgtaggaca cgcgcacctg
    53401 gaagaccgtc acgtccaggc cgaagagccc gcccgcgtag gcgagggacg acccccactg
    53461 accggcaccg gtctcggtag acaggcgctt cacgccctcc tgggcgttgt accaggcctg
    53521 gggcacagcg gagttgggct tgtggctgcc ggcgggcgac acgccttcgt acttgtaata
    53581 gatcttggcc ggcgtatcga gcagcttctc cagccggcgg gcgcggaaca gggggctggg
    53641 acgccattga cgcaggacgt cgcgcaccgg ctcggggatg tcgacgtagc gctcggtgga
    53701 gacctcctgg gcgatgatgg cgttggggaa caggggggcc agatccgccg gcccgagggg
    53761 ctgcagggtg cccggatgaa ggggcggcgg cagcggtacc ggcaggtcgg cggcgatgtt
    53821 gtaccaggcc ttgggcaggc gggattcgtc gagcagatac ttgatctggt cgctcacggg
    53881 cgatctcctt gaaatggact tgttgttatg cggacaggtg ccggacagcg ccgtgcgggc
    53941 gccgcccggt ggccgcgcag caggctgcgc ggcccttcgg atcagaccag ctcctgcagt
    54001 tcgatgtccc agcgggacaa caggaaacgc agcagggtct gttcttcgct gctcaggcgg
    54061 ccatccgatt tggccaccac cagcacggcg gagatcgcca ccttgcgcag ggcgggatcg
    54121 gcaatctcct cggccagcgg agccaggcgg gcttcgctga gcagatccac gcgaccacgc
    54181 ccgccttcgc tgagcagatc gcgacaacag tcgaggagca cctgcacgag ggtgtcctgc
    54241 tcgacgccga tgagggacgc agtatcgtgc tcgcggatcc agttgacctc ctggttgtgc
    54301 agacggccat cggcgaggat tgccagggcc accaggcggg ccagggcttc ggggctgttc
    54361 gtgggataag gacgcatcat ggtggagcac ctggagggga gaaaggcgat agggatgata
    54421 cgccgccact gtcggcggag atttgactta gatcggggtc cgaccgcgca cccgcccggc
    54481 cagcaccacc agcaccacga gggcggcgag gcccaccgcc gggccgttgc cccagcgccc
    54541 gtaaggcgtc gagccggtgt agccgcgcac cgccacctgc agggcaccgc gggtgaaggc
    54601 gggcagcgcg gcgctcaccg tgccgtcggg ctggaccagc ccggtcatgc cggtgttggt
    54661 ggcgcgcagc attggccgcc cgctctcgag cgcacgcagg cgggcgatct gcaggtgctg
    54721 gggctgggca aaggagtccc cgtaccaggc cagattggac aggttgagca tcagggtcgc
    54781 ctcgggcagg gcgcggatca gctcctcgcc gaagacgtcc tcgtaacaga tgttgaccgc
    54841 gatcttttgc cccgccaggc tcatgggcgg ctgcacctcg gcgccacggg tctggtcggc
    54901 catggggatg tgcaccagat cgtagaacca gcggaacagg ggcggcgagt actcgccgaa
    54961 gggcaccagg tgatgcttgg cgtaatgctg ggtcggcgag cgccccacac tgacggcggc
    55021 gttgaagatg cgcccgtccc catcccgcac gaaggcaccg aggatcgcgt cgccgcccac
    55081 cgcggcactg cgggtgaggg cgtccagata accgtcgggc agatgctcca ggagcagcgg
    55141 cagggtggtt tccggcaaca ccacgagggt cgccgggttg ttccgcagca gttccaggtg
    55201 gatgtccagc cagcgcggca gcatgtcggg ccgccacttc agatcctgct ccatgttggg
    55261 ctggagcagc gccacctgca gcggcgcgcc gacgggtacg gtccatgcca ccggacgcag
    55321 ggcggcgccc cccgcgccga caagcaggac cagacccgcg caggcgattc tccgccgcgt
    55381 cactgcttcg gccagcagcg cggcgatcca cgccagcagc ccgcccacgc cgaacacgcc
    55441 gaacaccggc gcaaagcccg ccagcgggct cggcggcgtc tggctgtagc cgctgaccag
    55501 ccacggaaaa ccggtgaaca gccagccgcg ggtccattcg gtgagcagcc acagcgcgcc
    55561 gaagagggcc gcgtccgccc atggccgccc ccgccgcaga tcggcgaaga gcgctcccgc
    55621 cccaccggcg aagagcgcca tcacggcaca gaacagggcg atggcgctcc ccgccagggg
    55681 catcggcatg ccgccgaatc ggtgcagggc cacgtacagc cacgacacgc cggcccccag
    55741 ggcgccgaag ccccaggccc agccgatgag gaatcccgcc cgccggcggg cgccgggcgt
    55801 gatggccgca tcggccgcgc ggcccagcag cgcgtacagc acggccaggg ccacggccgc
    55861 cagcggaaaa tactcgaatg gggcgtaggc gagcaccgac gccgcccccg ccgcccaggc
    55921 gaggaccgga cgcaggcggg acatcagccg ccgatcacgc cggcctgagg cgtgcgctcc
    55981 acgaggaggg aatacacgcg ccggctgtca gcccgcagaa tctgaaagcg caggttgtcg
    56041 aggacgatca cctcattgcg cttgggcagg cggccaagct tgcggatcag caggccgccg
    56101 acggtgtcgt actcgtcgtc gctgaaggtg gtgccgaagg cagcattgaa gtcgtcgatc
    56161 tcggtgccgg ccttgacgcg gtaacgcccg gcctggtcga ggcggatgtt gtcggcggcc
    56221 tcgtcgaaat cgtattcgtc ctcgatgtcg ccgacgatct gttcgagtac gtcctcgatg
    56281 gtcaccaggc cggcgacacc gccgtactca tccacgacga tggccatgtg gttgcggctg
    56341 acgcgaaatt cccgcaggag cacgttgagg cgtttggatt ccggcacgaa gaccgccggc
    56401 cggagcatgt cgcgcagatc gaaggcttcg ccggcgaaga cccgcagcag gtctttggcg
    56461 aggaggatgc cgatcacgtc gtccttgcct tcgccgatga cgggaaagcg ggagtgggcg
    56521 gcggtgatgg cgaagcgggt gatgtcctcg atgctgtcgt ccacgcggac gacgtccatc
    56581 tgggcccgcg ggatcatcac gtcggcgacg cgcatgtccg acacctgcag ggcgccctcg
    56641 atgatggcca gggcgtccgc gtcgaacagg ttgcgctcat gcgcggcatg gagcaggtgc
    56701 aacagttctt cgcgatcctc cggctcccgc gtgagcaggg ccgaaagtcg ttcaagcaat
    56761 gacggtttac tactggaggg ttccataaaa ggggtggatg agaaatgggc catgggcgat
    56821 ggacacatcg cccatggcgc attgctcacc ccgtcccgtc agtgctccag cgcgtagggg
    56881 tcgtcgtacc ccagttcggc caggatggcc cgctcgcggg cttccatgag ctcggcgtcg
    56941 gcgtcggcct catggtcgaa accttgcagg tgcagcatac catgcaccac cagatgggcg
    57001 taatgggccg ccaggggctt gccctgctcg gccgcctcgc gctccacgac gctgcggcac
    57061 agcaccagat cgcccatcag cggcaagcct tccggcatcg gcatgtcctc gccctcggcg
    57121 taggcgaagg tgaggacgtt ggtggcgtaa tccttgccgc gataatcccg gttgagttcg
    57181 cgcccctcgg cttcatccac gatgcgcacg gtgatgcgcg cggcgccgga ttcggcggcc
    57241 cgcagccagc gccggaagtc ggcccgcaag ggtacgccat cccggtgaca ggcatattgc
    57301 acggacagat cgaggcggcg gggcagcgcg gaggcggtca tgtggtcggg gccggggtgg
    57361 ccgaatggga ttcgtaggcg gccacgatgc gcgcgaccag gggatggcgc accacgtctt
    57421 ccttgaggaa gcgggtgaag gcgaccccgc gcacgtcggc gagcacctgg agggcttcgc
    57481 caaggccgct gcgctggccg cggggcaggt ccacctgggt caggtcgcca gtgatgacgg
    57541 cccgcgagcc gatgccgatt cgggtgagga acatcttcat ctgctccggc gaggtgttct
    57601 gggcctcgtc gaggatgatg aaggcgcggg acagggtgcg gccgcgcatg aaggccagcg
    57661 gcgcgatctc gatgcagccg cgctcaaaga gcttggcgac ccggtcgaag cccatcaggt
    57721 cgtagagggc gtcgtagagc gggcgcaggt aggggtccac cttctgggcc agatcgccgg
    57781 gcaaaaaccc gagccgctcg cccgcctcca ccgccgggcg ggtcaggatg atgcgctcca
    57841 cctgctcccg ctcgaaggca tccaccgcgc tggccacggc caggtaggtc ttgccggtgc
    57901 cggccgggcc gacgccgaag gtgatgtcgt gctcctggat ctggcggatg tagtccacct
    57961 ggcgcggcgt gcggccgtgg agttcggtct tgcgggtgag caaagccggc gccgagtgac
    58021 tggcctggct gcggttggcg agttcgatga ggcccagttg cacggtgtcg atgctcaggt
    58081 gctcgccggc cagatcatag aaatgctgca gggcctgcgc cgccagccgc gcctggacga
    58141 ggtggccgcg gacgctgaag cgctcgccgc gacgggcgat ctccacgtcg taggcggtgg
    58201 cgatctggcg caggttttcg tccagcacgc cgcacaggtt ggccagccgg gtgttgtcgg
    58261 cgggctcgaa ggccacttcc agagctttgt tcttcacgcg gggaggtttg gagaggacgc
    58321 gggcaccggc ccgaaaggcg ccattctagt ccttgagcac cagttcgcca cgcagggaat
    58381 ggggcagcgc ggagacgatg gtgacgtcca cgaagtggcc gatcatgcgc gaattgccgg
    58441 cgaagttgac gacccggttg ttgtcggtgc gcccggccag ctccgccggg ttcttcttgg
    58501 acgggccctc caccaggatg cgctgcaccg agccgaccat ggcttcgctg atccgcgcgg
    58561 cgtgggcctc gacggctttc tgcagacgcg ctaggcgggc cagcttgagg tcctggggcg
    58621 tgtcgtcggc catgtccgcc gccggcgtgc cgggtcgcgg gctgtagacg aagctgaagg
    58681 aggtgtcgaa gcccagttcg tcgatgagct tcatggtctg ctcgaagtcc gcctcggtct
    58741 cgcccgggaa gcccacgatg aagtcggacg acagggagat gtccggccgc gccgcacgca
    58801 gcctgcgcac cagggatttg tattcgagca ccgtgtagcc gcgcttcatg gccgccagca
    58861 cccggtcgga gccggcctgc acgggcaggt gcagatggga cacgagcttg ggcaggcggg
    58921 catacgcgtc caccacgcgg gcggtcatct cgcggggatg agaggtcgtg tagcgcaggc
    58981 gttccacgcc ggggatctcg tgcacgcatt cgagcaggaa ggcaaaatcc gccatctcgc
    59041 ctgcggcccc gtccagcggc gcacgccagg cattgacgtt ctgccccagc agggtgatct
    59101 ccttgacgcc ctgggcagta agccccgcca cctcggccag gatgtcggcc agggggcgat
    59161 agacctcgtc gccacgggta tagggcacga tgcagaaggt gcagtacttg gagcagccct
    59221 ccatgatcga cacgaaggcg ctcgcccctt ccgtgcgcgc cggcggcagc gcgtcgaact
    59281 tttcgatctc cggaaaggag atgtccacct gggggcgccc ggtggccttg cgctcggcga
    59341 tcagcttggg cagccggtgc agggtctggg gtccgaagac cagatccaca tagggcgccc
    59401 gctgcacgat ggcctcccct tcctggctcg ccacgcagcc gcccacgccg atgatcagcc
    59461 cagggttctt ctgtttcagg tgcttcaccc gccccagatc gtggaacacc ttctcctgcg
    59521 ccttttcgcg cacggagcag gtgttgaaca ggatcacgtc agcctcctcc ggattgtccg
    59581 tcttcaccag cgtctcgcca gcgccgagca cgtccgccat cttgtcggag tcgtactcgt
    59641 tcatctggca gccgaacgta cggatataca acttcttcat gacctttttc ttgaccaggg
    59701 tggattcggt ccactattat agcgggctca gtgtggtgat gtagctcaga cggttagagc
    59761 gacggattca taacccgtag gtcggcagtt cgattctgcc catcaccacc aacagaatca
    59821 aagacttagg ccaatccttc ggggttggcc tttgtcgttt ccgggctcgt tgctc
//
LOCUS       DAOQMM010000010        57638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_96_length_57638_cov_49.346092, whole genome shotgun sequence.
ACCESSION   DAOQMM010000010 DAOQMM010000000
VERSION     DAOQMM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 57638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57638
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96_length_57638_cov_49.346092"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..2301)
                     /locus_tag="PLH85_03055"
     CDS             complement(49..2301)
                     /locus_tag="PLH85_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB63564.1"
                     /translation="MTATIPSPDDVLELLDEDASAAVPGMADAWTVLVVDDDAEVHQA
                     TEFALRDAIVCGRRIALLHATSAAEARARIAGDDDIAVILLDVVMESPDAGLALVHTI
                     RETLGRSEVRIVLRTGQPGYAPEMKVIREYDINDYRTKAELTHTRLLTTLTAAIRAYE
                     QIRRLSVGMRGMREIALSGVQGEIPRSVAEFASVALGRIESLVGRPIQAAFCGPTGDG
                     GTLEDRLGILAGRGSYSCEEGALLSELRDAAWVESIRRARAQQASVFGDRTMCLFLGS
                     RYGKEACVLVDLDRPADEMLRHLVGVLAAHLRVAFENVLLLERLNFFAYFDQLTRLPN
                     RTCFLDRLDAALARLDGGGWVLGIIDIVRFSELNNALGHRSGDALLVDVTRRLGHRLG
                     PEAFLSRLAGDAFGVCWRTGHEEASAVLDVFATPFGVRGHAVPMRARAGFVDVVPVAG
                     GAVELLKRANLALAQAKQQGDQHCSFYASEMEERTQSRVQLLNELRSALHLGQGLELF
                     YQPQVRLGDKMAVGCEALIRWFKPDGEMVSPDVFIPLAEYTGLIVEIGDWVLAEACRQ
                     LVRWDAEGLPPIRVGINISAAQFREPDFVSKVLAQIHETGIDPSRLELEITESVAVDD
                     PESVARQLRELKALGFSVAVDDFGCGFSSLGSLNRLPFDRVKIDRAFVRELTPSTAEG
                     CIARMIVKLAESLGLKVTAEGVETESQAELLESMGCEEMQGFLYGCPMPAPTFQGWMK
                     SRFGTLFAER"
     gene            2481..3899
                     /locus_tag="PLH85_03060"
     CDS             2481..3899
                     /locus_tag="PLH85_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HNB63565.1"
                     /translation="MNTTERLASLDMTWLQLDRETNRLHVTGVLVFDTRLRRDRLEDI
                     LRRRLLRLDRFGQHVIRDTVGAIWESGRVDLDYHVQTFALPDAADPRHALQALVGRLA
                     STPLDPGRPLWQLHLVEKYRHGSAVVVRVHQCIADSIALIGVVQSLTDDHPTTPEDAI
                     PPAGQFDASFFEHLYAPLTQAMIEGIKLSGAAWAKYWSLLFYPQQVFDYARTNAALTM
                     EVGKLMGMRDDSRTCLKGTPQGTKLASWSAPLAASSVQAAARTHRATNDATLLACLAG
                     ALRRFFVRQGDGAEGVELRALVPVNLRGEKARDPLGNRGGILPLALPVGIDDPVTRLA
                     EIQRRLEDFEDAYRAHDTLGLFNLIGQTPRAVQAQALRLVSAKASALLCHVPAASEAR
                     YVDGARISEQMFWSPVTGDMGLSMSILSYAGHYQVGLMVDASMVPDPETITGQLGEEL
                     RLLRNAPASSRKPRSRKAASTS"
     gene            complement(3909..5327)
                     /locus_tag="PLH85_03065"
     CDS             complement(3909..5327)
                     /locus_tag="PLH85_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63566.1"
                     /translation="MRGVQPVLLGTDVLIFALLALVALVIWRARRSEPLRDAWRRVGE
                     SAVGMASLGVLLCFVLVGGLDSLHYRERLAPPAGAAPGEKGPLSTEVRSALDALAAPL
                     RARTEKTYSAPLALHLFARETVERPDGSQSREFPRLRHAGKDLRYPESDHVGDVIGRG
                     GLGLAAGLGVWAGVAAVVVLGVARSRGVPVGAAWRALSRGKGGLAWRALLLTLLGVLA
                     IAGPALMLAAEYHVFGTDKVGQDVLYLTLKSIRTALVIGTLTTLLTLPLAIALGILAG
                     YLGGWVDDLIQYVYTVLNSIPGVLLIAAAVLMMQVVIDTHPHWFDTAADRADARLLAL
                     CFILGMTSWTSLCRLLRGETLKLRELEYVQAARAFGVSTARILLRHVLPNLMHLVLIA
                     VVMDFSGLVLAEAVLSYVGIGVDPNTVSFGTMINAARMELAREPMVWWSLAAAFVFMF
                     VLVLAANLFADVVRDAFDPRSV"
     gene            complement(5324..6301)
                     /locus_tag="PLH85_03070"
     CDS             complement(5324..6301)
                     /locus_tag="PLH85_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63567.1"
                     /translation="MLAYILRRLLYAPLILIGVNLLTFALFFVVNTPDDMARMQLGVK
                     RVTPEAIERWKVERGYDKPLMYNTAAQGLDALTDTIFFRKSVRMFVFDFGRADDGRDI
                     AQEIRSRMGPSLAVALPTFILGLFVTVSLALLLVFFRGTYLDFTGVVLCVAAMSISGL
                     FYIIGGQWLVSKILHLVPISGYGEGLDASRFLILPVAIGVIAGIGGSARWYRTVFLEE
                     ADRDYVRTARAKGLSELQALFGHVLKNGMIPILTGVVVVIPSLFMGSLITESFFGIPG
                     LGSYTIDAINAQDFPVVRAMVFIGSVLYIIGLILTDLSYTLADPRVRLE"
     gene            complement(6365..7216)
                     /locus_tag="PLH85_03075"
     CDS             complement(6365..7216)
                     /locus_tag="PLH85_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB63568.1"
                     /translation="MSNGMVVVLAVLVFALVTLAKGVRIVPQGEEWIVERLGKYFGTL
                     RPGLNLIIPYLDRVAYRMVTKDIILDVQEQEVITRDNAVILTNAIAFLKVTDPVKAVY
                     GVTDFSEAIRNMIMTTLRSIIGEMELDEALSSRDKIKARLRESIADEAVDWGLTVKSV
                     EIQDIKPSESMQRAMEQQASAERERKAVVTRAEGAKQAAILEAEARLESSKRDANAQV
                     MLAEASAEAIRRVTAAVGTETAPMLFLLGEKYIASLERLGASENARVVLLPADLQDAV
                     KGILGKR"
     gene            complement(7227..7667)
                     /locus_tag="PLH85_03080"
     CDS             complement(7227..7667)
                     /locus_tag="PLH85_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNB63569.1"
                     /translation="MVFEWWHWLVLGIALVIAELAVPAFFLIWFGLGGLLVGLALLVL
                     PLGGTAQIALWVAASLAMVFLWFKVFRRGEFKTLIGQSDAHVLGEIGLLSQAVAPFER
                     GRVRFQKPVLGADEWVCRADEAIAAGERVKVVAVEGSFLKVSKV"
     gene            complement(7817..8110)
                     /locus_tag="PLH85_03085"
     CDS             complement(7817..8110)
                     /locus_tag="PLH85_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HNB63570.1"
                     /translation="MSWFSHTADGGLVLNLHIQPGAKKTECAGLHGEALKIRLAAPPV
                     DGKANAALVAYLAEMLRVPKSAVVLVSGETSRAKRVRVSGAEEGRVAALAARG"
     gene            complement(8118..8690)
                     /locus_tag="PLH85_03090"
     CDS             complement(8118..8690)
                     /locus_tag="PLH85_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNB63571.1"
                     /translation="MFGSLILTIVDTLGSLLAGVLLARFVMQWQRVSFRNPLGQFVLA
                     TTDWLVIPLRRLVPGLFGLDMASLLPAWVVQTLVVFVTLWVHGVIGRADPLSLLAVMW
                     GVGLLETVKVALYLTMGVVLMSAIFSWVNPHAPLAGVVYALADPFLRPFRRLVPLIGG
                     VDLSPIVLLFVLQIALSALAFLRFSLLGMA"
     gene            complement(8703..9515)
                     /gene="proC"
                     /locus_tag="PLH85_03095"
     CDS             complement(8703..9515)
                     /gene="proC"
                     /locus_tag="PLH85_03095"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNB63572.1"
                     /translation="MKITFLGGGNMATALIGGLVKQGFDATGVQVVELSAEGREKLAT
                     QFGVRAVDALDDTALACDVLVLAVKPQQLRAAVAPFAGRLGAQVVVSIAAGLRLADIG
                     RWLGGYRTLVRAMPNTPALIGAGVTGLYADASVSAEGRAAAEQVLAAVGSTVWVPDEA
                     QIDAVTAVSGSGPAYVFHFIECLEAAGTALGFDAATARKLALDTVLGAAKLAAGSDEP
                     PSVLRERVTSKGGTTEAALKSFAADGLPAIIARAVAAAEARGRELGEQLGRD"
     gene            complement(9548..10231)
                     /locus_tag="PLH85_03100"
     CDS             complement(9548..10231)
                     /locus_tag="PLH85_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNB63573.1"
                     /translation="MTGISVGLQKVRDRISTAAQAVGRDPHGVTLLAVSKTWPAECVR
                     AAAAAGQRAFGENYVQEGCAKVDALADPDLEWHFIGPLQSNKTRSVANRFVWVHSIDR
                     LRIAERLSAQRDVHLPPLNVCLQVNVSGEASKSGVAPGEVADLAAAVAALPRLRLRGL
                     MAIPEPTDDVALQRRRFASVRALRDELNAAGFGLDTLSMGMSADLEAAIAEGATLVRV
                     GTAIFGARN"
     gene            10362..11405
                     /locus_tag="PLH85_03105"
     CDS             10362..11405
                     /locus_tag="PLH85_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNB63574.1"
                     /translation="MDITELLAFTVKNKASDLHLSAGLPPMIRVHGDVRRINLPALVQ
                     KDVHGMVYDIMNDTQRKQYEEHLETDFSFEIPNLARFRVNAFNQNRGAAAVFRVIPSK
                     VLTLEELNCPKIFKDICNQPRGIVLVTGPTGSGKSTTLAAMIDYINENEYGHILTVED
                     PIEFVHESKRCLINQREVARDTLSFNAALRSALREDPDKILVGELRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKDMVRAMLSESLRAVISQTLLKTKDGSG
                     RVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNLGMQTLDQSLTDLIRRNQISAVE
                     ARVYAANKDSFTG"
     gene            11483..12616
                     /locus_tag="PLH85_03110"
     CDS             11483..12616
                     /locus_tag="PLH85_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNB63575.1"
                     /translation="MERDQALKFMHDLLKLMMQKNGSDLFITTGFPPAIKVDGKITPQ
                     SNQVLSAAHTAELARVIMNDRQAAEFEATKECNFAISPAGIGRFRANAFIQQGQVGMV
                     LRTIPQRIPNFTELGLPPVLKDIAMSKRGLVVFVGGTGTGKTTSLAAMVDYRNEKSYG
                     HIITIEDPVEFVHPHKNCIVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETM
                     DYAIAFAETGHLCLATLHANSANQAIDRIINFFPEERRAQLLMDLSLNLRALISQRLL
                     PMCDRKGRVPAVEVMLNSPLISDLIFKGEIPGIKDVMKRSAELGMQTFDQSLFDLYER
                     GLITYEDALRNADSMNDLRLQIKLKSRRGDNDMLSGIQNLDIV"
     gene            complement(12746..13918)
                     /locus_tag="PLH85_03115"
     CDS             complement(12746..13918)
                     /locus_tag="PLH85_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNB63576.1"
                     /translation="MDFFAPVPRGLEPLLVDELRSIGAVDPKALPGGVQFSGDWAVCY
                     RANLESRLATRVMLQVASGRYRSEDDVYRIAYAPTWARWFTADQTLRVFVTAHKSPLR
                     SLEFTTLRIKDAVCDHFRSVAGRRPNVDTKAPDVRIHAFLSADTVTLYLDTSGEPLYK
                     RGFKPAAVEAPLKENLAAGILRLTGWQPGSEALVDPMCGSGTFLIEAAQMALDVAPGL
                     GRRFGFEKFKNFERDQWLPIRQAAEARRQAPRPLPIHGSDILEAQERKSIVNLRSAGL
                     DGCVTVSRTDMLELAAPAASGVLVANPPYGVRLSDMSELRDFYPRLGSALKAHWAGWR
                     CYFFSGDPELPKGVRLKASKRTPLFNGAIECRLYEFAMVAGSARKPKGEGGAADAD"
     gene            complement(13941..14351)
                     /locus_tag="PLH85_03120"
     CDS             complement(13941..14351)
                     /locus_tag="PLH85_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNB63577.1"
                     /translation="MLIVKALHIIFVTSWFAGLFYLPRLFVNHAMVTDPATIERIALM
                     EHKLYRFMTPLGILAVLLGMWLWFGYGFVGGWLHAKTALVTLLIVYHLYCGRLLRAFA
                     EGRNTRSHVWYRFFNEIPVLVLFAVVFLVVLKPF"
     gene            complement(14388..15260)
                     /locus_tag="PLH85_03125"
     CDS             complement(14388..15260)
                     /locus_tag="PLH85_03125"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB63578.1"
                     /translation="MKTKRVLIVGSGDVARRLIPWLARRCRVFAVIRRPEEREALRAL
                     GAVPLVADLDDRASLDRLAGVADAVLHCAPPPGQGSDDPRTRSLLAALSRRASLPRRL
                     IYISTTGVYGNCGGAWLDETRPVAPASSRGVRRVAAERRLRSFGTRSGCGVSILRAPG
                     IYAVDRLPVERIRRGDPVLAADDDVFTNHIHADDLARLLGLSLFRGRANRVYNACDDS
                     QMRMGDYFDAVADAFALPRPQRLPRAELIAQVSPLTLSFMSESRRIRNDRIKRELRVR
                     LIHPTVADALKRLA"
     gene            15297..16328
                     /locus_tag="PLH85_03130"
     CDS             15297..16328
                     /locus_tag="PLH85_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNB63579.1"
                     /translation="MSFQVTLQPGAHQFAASADETLLQSALAAGLLLPYSCRDGACGV
                     CKGKVVSGRVDLGRYSESSLTASERAQGMALMCCARPLTDLELEVRQVNLVGEIPVKK
                     MPCRVQKLQRLCDDVIVLDLKLPASERFQFRAGQYIDFLLADGKRRSFSIANAPLDGA
                     DHLEIHVRLVPGGQFTTHVFERLKEKEILRFEGPLGSFGLQPGAAKPVVLLAGGTGFA
                     PIKSIVEQAIADASTRPMTLYWGSRDRAGLYQFDEARAWEARLPGFRFVPVLSDVPAG
                     EAWDGRRGLAHHALMEDLPDLSGHEVYACGSPAMIDAARSDLVARCDLPPDAFFADAF
                     SFSNDARGA"
     gene            16354..17493
                     /locus_tag="PLH85_03135"
     CDS             16354..17493
                     /locus_tag="PLH85_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB63580.1"
                     /translation="MSHTALITREPVINKQYKITANRLVVHAKSVGEVVQTLDGVADV
                     WPATHPVFISLYRLVPTPDLLEWIAPANAMVEIPTQAIAHPLTQQLIPQLRAQGVSLN
                     LTWYHPDVTLPPDVDWRFVIMDAHKHPAPLDPPGLSIAWGLEDFPAFRAAIGSGYDGA
                     SGWFFLKGLNAGGKLAPSHAQIVRLLNLVRRNAEVKEVETVLKQDVALSYKLLRYINS
                     AGFGLMCEIQSFRHAVTILGYDKLHKWLSVLLVSASRDPSAPALMQTAIARGRFMERI
                     GEAFFDRNQLDNLFITGAFSMLHLLLGASLHSALEEMHLPEPIADALLSGEGVYAPFL
                     KLARALEGFDDKHLTELAADLHISADVINRAHLEALGFADSLQFG"
     gene            17738..19786
                     /locus_tag="PLH85_03140"
     CDS             17738..19786
                     /locus_tag="PLH85_03140"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB63581.1"
                     /translation="MPALPECAATLLKVVAEGTTSSEDISLRICRDPILFLEILTTAP
                     LGRDERSTPLKAVERRIAELDPGLLQAWFLRHAVPAPGQRTSIGSLRNLSLHSQLVAE
                     LAFHLARETAYPTPEDAYVAGLLHDVGQLWLAEHQEGYVTMRAALSDEKALSAAEWER
                     YGADHSTISAELAHQCGLGALVADAILLHQTSPVQLRSAHLLVRLIAAAEELASYRQT
                     QSALSLDIASHLAGLDGSVLVSLLAETGPQVNDAASDFGVAIEPAMLALPWALGPDVA
                     PAPEAGTTGISRAALNAFARGTFASAEEARNGLARAARLLLGLDAPALFVRSDNGLAL
                     VGCASATHPSLDQMCVTLADDTSAIAEAARSNAETRYRCDERPPGRSAIDWQVSRWLG
                     SDGMLCVPWKNHHDAGVAVFAFASADSDPPDSTRALRRDLVLSATLRLSEERASQHLR
                     DDLEQTISRRYAEHARRLAHEASNPLTVIKTYLGIIDERIEDAAIREQLGVLNDELDR
                     IGNLVRRAPDFREPAADGPAQSGVTEILHDLRALYGDALFARRGIQFEVRTTPDLPSA
                     AIAPDALKQVLVNLFKNAAEAIPEGGKLVVTVGTGINANGTSCLEIRVVDNGPGLPPG
                     RIETLFAPVPGGAGRTEGGIGLPVVRDLITAHGGTVLCRSQLGSGTVFQIFIPLAGTP
                     "
     gene            19836..22037
                     /locus_tag="PLH85_03145"
     CDS             19836..22037
                     /locus_tag="PLH85_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB63582.1"
                     /translation="MVIPGERPFFPPETGNRGAPDPLIRILVVDDQPSLRRSVVALLQ
                     GDDRLIEDCGSVAETLVRLGAASYDLIILDYRLPDATGLAVMNWLSSHHRNESVIVIS
                     GEDSMEAAIAALRRGAQDFLRKPYDPDQLRRAVQSVLGKRQQERSHRLTLQRLQSSEH
                     MHRYLVESSPDLIFTTTPDGRFNYINPRIESLLGYRRNSLIGQHFSEIVHPEDLERTR
                     QTFHERRTGQRAAANVEVRLTRSPFGPDQDGEAGPLPFVLNAHGIYHDEDGTTPGEYL
                     GTYGVARHAPPLRLTALAGNPPLQRDPLTRLPGRELLFDRLDLAVAQAKRRKGQIAVM
                     QVDVDRFKDVNERFGQGEGDTLLCAIARRLQRCLRRGDTLSRQGGDEFTILLPDVRGK
                     DNAQAIAGKIHQAFEQPFSLGKDEVRLNCSIGVALYPEDGDDAESLTQHAAIAMFQAK
                     RNGGESHEFFEPGMHSAYRARISLETELRLAISNRELELFYQPRVSLSRNQVTGMEAL
                     IRWRHRNHGLVAPSRFLQLAEEVGIMPDISRWVLETACAQLSAWRRLYPELRLATNLS
                     LRDFDRADLNDAIYKALAQHNLPPDSLELEITENLLLEDHEFLAAKVQGLRDMGVGLS
                     IDDFGTGLSSLSLLHRFPVTRLKIDRSFVRNINGQGSHPVINAITGIARGFDVQVSAE
                     GVERGDQVSVLETAGCDEMQGYFFSKPLGAEAATRMLQDFRPAAMLQTAPG"
     gene            22108..22332
                     /locus_tag="PLH85_03150"
     CDS             22108..22332
                     /locus_tag="PLH85_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63583.1"
                     /translation="MGTFRLILAHARAPQQSTVVAQHVDPVWLKQRGYEIARSLGDGS
                     ALWGRRADAGNLSGLELNCRGGHALRIEAA"
     gene            complement(22355..>22609)
                     /locus_tag="PLH85_03155"
     CDS             complement(22355..>22609)
                     /locus_tag="PLH85_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB63584.1"
                     /translation="PQAEVRPPADGRAAPARGYVVQVGGFMEPAAAEDLRARLARGGV
                     PVQLESRVMVGPFADRREALAAQSRLREQGYAPGVIQPSR"
     assembly_gap    22610..22709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22710..23236)
                     /locus_tag="PLH85_03160"
     CDS             complement(<22710..23236)
                     /locus_tag="PLH85_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63585.1"
                     /translation="MTDERNNPPVQDEKDDSRRQLWTRAAVAVGLIGLLLGSLIVFDE
                     ASRPPEAEEAKVPAKPIGPAPVAGRESEPPPDVVRAGGEAADQASSAPPLPVAADQGE
                     PPKAARKADDAADETRPVVGAGARTVLPESPAVASSRSVTLKEDTLRGGVAPLAATPA
                     AAPAGAPSAPAVAPQA"
     gene            complement(23233..23865)
                     /locus_tag="PLH85_03165"
     CDS             complement(23233..23865)
                     /locus_tag="PLH85_03165"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNB63586.1"
                     /translation="MIEIGVREGIWIAIGVLTLYLAFVLIRLALHRARPLAEEPQTLV
                     VDAEAPAPQGVAPAALTPSGEDVASIRTPAPTEDADVGDIEPLDDYADPRTGRGESSA
                     AEQPDSLPTFDLIEFFQLEVQQLRRDVAQLRGEQDQLRREIASVRRDLDEIQVADGRS
                     HFVRPVSPQHSEAMVLAERGMTAAQIADRCGIAVAEAELVCALARREVHE"
     gene            24024..24797
                     /locus_tag="PLH85_03170"
     CDS             24024..24797
                     /locus_tag="PLH85_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNB63587.1"
                     /translation="MFFLKKIAAALLLPPFLPFLLIIAGLLLLHRQPRTGRLLAWCGV
                     VTGIALSLPASVGLLVTPLERDAALDVRDLPAAEAIVILGGGTRHYAPEFGGQTVNRL
                     TLERLRYGALLARHSRLPVLVSGGSPTPGIAEGILMRRVMEEEFRVSVRWVESQSLDT
                     AENAAFSVPVLRAAGVRRVLLVTHAAHMRRALEAFEAAGMHAIPAPTAFFHGAGPADE
                     VVDFLPGMNAAFAGGYAAHEWLGLAARRLTDLGGGLIRR"
     gene            complement(24787..25584)
                     /locus_tag="PLH85_03175"
     CDS             complement(24787..25584)
                     /locus_tag="PLH85_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB63588.1"
                     /translation="MTSPVTLDLQDGIATLTLNRPTALNALSVDMMKALAEATGQLQR
                     RTDLRAVVVTGAGEHFMAGGDLKDFATHLHLSAESRIATFRALIEQYINPSVEALAAL
                     PVPVIAQVRGACAGFGLSLVLGCDMAVAAGNAYFTTAYASIALSGDGGVSWFLPRILG
                     RRKAFELLLLADRFGADEALRLGVVNRVVPPEELEAATDALVQRFVKGPREAYAEMKR
                     LLVASPEQSLSAQLQLEAEAFARSSARPNFDEGIRAFLEKRKPGFNA"
     gene            complement(25658..27304)
                     /locus_tag="PLH85_03180"
     CDS             complement(25658..27304)
                     /locus_tag="PLH85_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HNB63589.1"
                     /translation="MMPIGDGPYAYPLLIKQLLRMPLVHAPDQEIVYRDQVRHSYREL
                     AARIGRLGSALSRLGVARGDTVAVADWDSHRYLEAYFAVPMLGATLMTVNVRLSPEQI
                     AYTLDHCEAETLLLNVDFLPLLEGIRGRLPKLKRLILLADGVCDPVAAGALAEYEALL
                     ATGRPDHAFPDFDEHTRATTFYTTGTTGLPKGVYFSHRQLVLHTLAGLAGLASAPGTQ
                     RFDRGDVYMPLTPMFHVHAWGLPYVATLLGVKQVYPGRYVPDEILALRAREGVSFSHC
                     VPTILQMLLLAPSARDMDLSGWKMVIGGSALTAGLATQAVARGLDLFAGYGMSETCPI
                     LTLAQLKPEELELPPEQTVSARCRTGLPLPLVDLRVVDADGTEVPHDDHAQGEVVVRA
                     PWLTQGYTKNPEATAQLWAGGWLHTQDIGRISPAGSLQITDRIKDVIKSGGEWISSLE
                     LETLVSRHPAVAEVAVLGVPDARWGERPLPVVVRRAGCEAVDAEAIIAHLRACAEAGQ
                     ISRFAVPERVVFVEALEKTSVGKLNKRLMRERYAVADGPR"
     gene            complement(27427..28623)
                     /locus_tag="PLH85_03185"
     CDS             complement(27427..28623)
                     /locus_tag="PLH85_03185"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNB63590.1"
                     /translation="MSKQIQEAYIVAATRTPVAKRNGMFRNVRPDDMLAHVLSAVVGK
                     VPGLDAREIGDVIVGCAMPEAEQGMNVARIGVLLAGLPDTVPGITINRFCSSGLQAVA
                     DAAARIRLGEADVMIAAGTESMTVMPQMMGNKISLNPAVFAKDENVAIAYGMGLTAEK
                     VAQQWEISREAQDSFAVQSNHRAAAAIAAGAFRDEITPYTVRTHVPGAGGVVRVSERV
                     CDTDEGPRPDASVVGLGKLRPVFAARGSVTAGNSSQMSDGAAAVLLMSEAAVKRYGVT
                     PIARFVSYSVAGVPPEIMGIGPIEAIPRALKLGGVAQSDLDWIELNEAFAAQALAVMK
                     QLDMDPARVNPQGGAIALGHPLGATGAIRTATLMSAMQRGQARFGMVSMCIGTGMGAA
                     GIFERV"
     gene            complement(28688..31072)
                     /locus_tag="PLH85_03190"
     CDS             complement(28688..31072)
                     /locus_tag="PLH85_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNB63591.1"
                     /translation="MSRLIIRKVAVLGAGVMGAQIAAHCANANVPVILFDLPAKEGDP
                     NGIAKKAVDSLKKLEPAPLATRDRAAAIELGNYGADLEKLRDCDLIIEAIAEKMEWKL
                     DLYAKVAPYVGDQAIFATNTSGLSINTLAEGFPEAARSRFCGIHFFNPPRYMALVELI
                     ATRSTHADTLDALEAWLTTRLGKSIVRAKDTPNFVANRVGVFSILAVMHHTARFGLGF
                     DEVDALTGPLIGRPKSATYRTADVVGLDTLAHVVGTMQATLPDDPWFPYYRNPDWLAA
                     LIAKGALGQKTGGGIFRKVGKAIHVLDLAAQDYRPSAGTVAPEVAAILKNRNVAERFA
                     QLRASEHVQAKFLWAIFRDVFHYVAVHLGDIADNARDVDFAMRWGFGWTTGPFETWQA
                     AGWQSIAEAVKADIAAGATMASAPLPAWVFDGRTGVHGPAGSWSAADQTDKARSTLAV
                     YGRQLYPEQVLGEAPADAGVTLWENEGVRLWHLPAKDARIGILSFKSKMHAIGDEVLD
                     GVLEAVARAERDLDGVVIWHEAPFAVGANLQQVAEACKGGQFDVLERTVAKFQQASMR
                     LKHALVPVVAAVQGMALGGGCEFLMHASHRVLALESYVGLVEAGVGLIPAGGGCKEFA
                     LRAAELASRAAGGDVYPFIQNMFNTVAMATVSKSAVQAVELGFAKPSDDIVFNPRELL
                     HVALHRARALAEAGYQPPLVRKDVTVAGRNGIATCEMMLINMQAGGFISEWDYRVARA
                     AATALCGGEVETNAKVSEQWILDVERALFLDLLKSEKTQQRIVHMLETGKPLRN"
     gene            complement(31243..31920)
                     /locus_tag="PLH85_03195"
     CDS             complement(31243..31920)
                     /locus_tag="PLH85_03195"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNB63592.1"
                     /translation="MGRLEHPQPDAKTRILDAAERLFMERGFAATSVRMITGAAGVPV
                     ALVNYHFGSKQGLMEAVYARALGNRGSRVSYLDRLEAEADGRPIAVNVLVDAFLSSAL
                     RLTRKDSIPGEVFKQLIGRAFYEPGPGTEAFFPDEYREAVDRYRQAFMRALPQLSQED
                     VVWRMYFLVGIVAYAMAGKDVMRMTETYALADQGQPEAILARLRPFVVAGFLAPPGAL
                     PSIAEIP"
     gene            complement(31986..33275)
                     /locus_tag="PLH85_03200"
     CDS             complement(31986..33275)
                     /locus_tag="PLH85_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB63593.1"
                     /translation="MKMKTLARMMPILAVAAATGEASAAGFQLLEQNASGIGNAYAGS
                     AAVAENASTIYFNPAGMTQLKDREFSAGIAVVRPSFRFANENSSAGALTSTGNGGDAG
                     STGFLPNLYASWALNKDLYVGLGIGAPFGLKTEYDNPWIGGAQSVRFEIKTVNINPSV
                     AYRVNEKVSIGAGINWQRIDVDYTRVLGTNSAPLGVTVPFPLNTSTGTFSANDSTWGW
                     NAGALFTLSETTKVGVSYRSKMRYTVKGDLTVQGPYAPLNAARSGPANATVELPDVFV
                     LSVVQQLDPRWTMMGDISWTGWSSIPHVDIINSNSGTTAQRLDTEFRDTWRMALGATY
                     KVNDAWKAKFGVAYDQSPVEDPHRLVSLPDSNRTWYSVGGQWKTPAGSLVDFGFAYLR
                     VNTSPIHNDQSSALRGVVDGHYNSNAFIAGAQYSLAF"
     gene            complement(33402..33809)
                     /locus_tag="PLH85_03205"
     CDS             complement(33402..33809)
                     /locus_tag="PLH85_03205"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB63594.1"
                     /translation="MSDSDSRLPEGKIPVLRVMPMPADLNPAGDVFGGWVMAQVDIAG
                     AIPAKRRARGRVATISVNAFLFKQPVSVGDLVSFYATIVKVGRTSITVDVEVFAERNP
                     ENPVVVKVTEATLTYVAVASSGVKREIPPEEPA"
     gene            complement(33829..35760)
                     /locus_tag="PLH85_03210"
     CDS             complement(33829..35760)
                     /locus_tag="PLH85_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNB63595.1"
                     /translation="MDLKHWTLERDADGLAWATLNVADAATNTLGRAVLDELGALLDD
                     LDRQPPTGLAIRSGKPAGFIAGANIEEFTRIDSAEAARTLVNRGWALFNRLAAVTYPT
                     LALIRGHCMGGGLELALACRYRLTVDEPGTRLSLPEVMLGIYPAWGGMLRLPRLIGPA
                     AALDLMLTGKSVDALKAKRLGLADDCVPPRVMDSAARVVLLSGRSPRRLPFGQRLLNG
                     PLKGVVAARARTTVAAKARPEHYPAPYAIIDLWANHGGNALAAPAASPSSLEAIFRSP
                     TAKNLIRVFFLQERLKGFGKDTDFRPQRVHVVGAGVMGGDIAAECALRGMVVTLQDQS
                     AERIAPAIARAAKRFERKFRGKRREARFALDRLIPDPAGHGVARADVIIEAIFENLEA
                     KRTLLADLERRARPDAVLASNTSSLRIGDIATALREPARLVGIHFFNPVAMMPLVEVV
                     RTGHTADAPLRCAAAFVRALDKLPLPVRDEPGFLVNAVLGPYMYEALRCVDEGIAPAT
                     VDAALTAFGMPMGPIELVDTVGLDIAVAAGTALAGADAVLPRTLADKVAAGQLGRKSG
                     QGFYLWQNGKAQKSAPGPLPDGLAERIVLPLRQAVQACVDKGVVADADLADAGVIFGT
                     GFAPFTGGPLHDLASRARP"
     gene            complement(35760..37040)
                     /locus_tag="PLH85_03215"
     CDS             complement(35760..37040)
                     /locus_tag="PLH85_03215"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNB63596.1"
                     /translation="MTTPVYIIDGARTPFLKARNAPGPFAASDLATAAGSALLMRQPF
                     APDQLDEVILGCASPSPDEVNIGRVVALRMGCGVKVPGWTVMRNCASGMQALDSAIAN
                     LQTGRSQLVLAGGVDALSRAPLLFSDAMVRWLSGWYAAKKFGQKLAHMRHFRLGHLAP
                     VIGIMKGLTDPVVGQLMGQTAENLAWRFGITRDDMDAFAVESHRRVAAAQDAGHFAEI
                     VPLIDRDGTLYTADDGLRRDASTSGLAKLKPFFDKHYGRVTAGNSSQITDGATWLLLA
                     SEAAVVRHGLTPLGRIADSQWAGLAPEQMGLGPVHAATPILQRHGLGLHDVDLWEINE
                     AFAAQVIACRRAWQDTGYCREQLGLDGALGALDVARLNVDGGAVALGHPVGASGARIV
                     LHLLHSLRRTGGRRGVAAICIGGGQGGAMLVEAL"
     gene            complement(37056..39530)
                     /locus_tag="PLH85_03220"
     CDS             complement(37056..39530)
                     /locus_tag="PLH85_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB63597.1"
                     /translation="MWWFLVFIPPLVVALAYGRAPFFAWVGAMLAWLAAWTAFGGLPP
                     GAALGVGAVVAVAGALFLVPALRRGLVTAPIFRAFRAALPTMSQTERDALEAGTVWWE
                     GELFAGRPDWRTLLAYPVPRLTDEEQRFLDNETAELCRLTQDWDCTQRQDMPAEVWQY
                     IKDKGFLGMIIPREYGGKGFSAFAHSQVVTKLSTRSSAPAVTVMVPNSLGPAELLLHY
                     GTPEQKAHYLPRLARGQEIPAFALTSPWAGSDAASIPDAGVVCRGTWQGREVLGMRVS
                     FDKRYITLAPVCTVFGLAFRLFDPDRLLGDVEDLGITCALVPHDHPGVKIGRRHLPLN
                     AVWMNGPIQGQDVFMPLDFIIGGPTMAGQGWRMLMECLAAGRSISLPGSNTGMQKMTA
                     RAVGAYARVRYQFKTAIGRFEGVEEALTRIGANTYLCDAARVMTAGAIDLGEKPSVVS
                     AIVKYHVTERARQTVNDGMDVIGGKGICLGPQNFLGRAYQQIPVGITVEGANILTRSL
                     ILFGQGAIRCHPYVLKEMNAAREGDLAAFDHAFWRHIGYTLSNAARALVTGLTGSHFV
                     SVPDGVAPETRRYYQQLSRFSAAFAFLSDISMGTLGGALKRKEKLSARLGDILSLMYL
                     ASATLKRYEADGRPTADAPLMHWAIWDCMFKAQNAFEGAIANFPNRFFAMLLRRLVVF
                     PLGRPYVVPSDRLGHEVARLLIEPSPTRNRLTSDVYLPTDVEEPVGALEAALLATVDA
                     EPVEARVRRAQRDGQFRPTVSVGGGVDAVYDQALAAGVITAAEHALIKRRGVLRDKVI
                     RVDDHPYDFGLRDALATVDVPARQAA"
     gene            complement(39593..40267)
                     /locus_tag="PLH85_03225"
     CDS             complement(39593..40267)
                     /locus_tag="PLH85_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNB63598.1"
                     /translation="MKDNVSKNGSDTRERILDAAEVLFMEHGFDGTSMRMITVQAAVN
                     LAAVNYHFGSKELLIQDVFRRRLTDLNKARLEALDALERQAGDAPVKPSRIVDAFFGT
                     ALRMAADVDGGGHTFMRLLGRTYTEPNEFVRKFLAEEYAECVERFLAALYRALPEVDR
                     HEILWRFHFMMGAMSYAIAGTDALQLVTGKFDDENPSRLAPRLMSFLLGGLRAPLADF
                     GPQPDA"
     gene            complement(40695..>41231)
                     /locus_tag="PLH85_03230"
     CDS             complement(40695..>41231)
                     /locus_tag="PLH85_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63599.1"
                     /translation="LKRFDVCRTWGPLRQPYRSGVWSGARVNHPPASDRGEATVPRER
                     VRKLMQGVHDACVGLPEGAVGVNAVEDAVAKGVGLDVGPCGVKRGPEPLRQGGPGGTV
                     IIGALRTVRTPGAGGETAIVRAQVGVHDGLMHAPIVQIHLVLGAIHDLAVTGFADEGL
                     RGDPESGKAGVARTTGAR"
     assembly_gap    41232..41331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            41605..42807
                     /locus_tag="PLH85_03235"
     CDS             41605..42807
                     /locus_tag="PLH85_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNB63600.1"
                     /translation="MEQITTTSNVVFVLLGAIMVLAMHAGFAFLELGTVRKKNQVNAL
                     VKILTDFGVSALAYFFIGYTLAYGVNFFSSAEVLAQKSGYEMVKFFFLLTFAAAIPAI
                     ISGGIAERARFHPQSVATFLLVGFVYPFFEGIAWNDNLGLQPWLEAAFGAKFHDFAGS
                     VVVHAVGGWIALPAVLLLGARHGRYTKDGNISAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQTMDKVSGLVALNSLMAMVGGTMAAMVLGKNDPGFIHNGPLAGLVAVCAGSDLMH
                     PVGALVTGAVAGVLFVQLFTITQNRWKIDDVLGVWPLHGLCGAWGGVAAGIFGSQALG
                     GIGGVSLAAQIIGTLAGIVVALAGGTVVYGGLKALIGIRLDQEEEWEGADLTIHKITA
                     TPDRETHW"
     gene            43268..44824
                     /locus_tag="PLH85_03240"
     CDS             43268..44824
                     /locus_tag="PLH85_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB63601.1"
                     /translation="MHRTNLRSTTRPLTLSALAISLLLLSGCASMKPEPLQPAEIVST
                     VDADRARLMQGVEPLTGVLSLEEAIARAIKHNAERRYRIMEEALAQGTFDASTYDLLP
                     RLVASAGYRDRNNDLISHSTDSVTGAPSLANPFISTGRTATTTDLTLSWSLLDFGQSY
                     YAAKQNADRLLIAQERRRKALQLLVQDVRTAYWRLVAAQSLHEAVGQGIREAEAALGD
                     ARKAEKEGLRNPMEPLRYQRTVLENIRMLETVQQELSTARTELAALVNLPMSTEITVA
                     PPSAVLQKAWLDLPVARLEEQALMRNADLREGILNSRIARQETRRTLLKLFPGLSFNY
                     GTKHSTDSYLINQTWNEAGVQLSFNLFNVFSAPAQMRLSEAGVALADQKRLVTQMAVL
                     TQLHVARLQYANAIHQFERADEIADIEARMAGHARNQSDAEKQSRLDRVSQSTSYVLA
                     QLRRYQALSNAQSAASKLQATLGLEPVVDAGAERPLPELTDSVRKALDAWQQAALPDA
                     GGSATGRDAP"
     gene            44832..45629
                     /locus_tag="PLH85_03245"
     CDS             44832..45629
                     /locus_tag="PLH85_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB63602.1"
                     /translation="MRLRSIALIINALLVTPAWSQTPPPRSAAAPAPVASNPGSALEN
                     REIRAQLAPRRYTTLAAEIGAKVSHLPVPEGGAFKAGQSLVQFDCSLQQAQLAKARAA
                     LAGADKTWQANRRLADLNSVGKVELETSEAEVLKARAEVSANATLVGKCAIAAPFAGR
                     IAEQKVREQQYVQPGQALLEILDDSALELEFIVPSRWLAWVRTGTAFQVRIDETGRSY
                     PAKVQRIGARVDAVSQSVKLNAVIDGRFPELIAGMSGKVQMTPPANP"
     gene            45649..>57638
                     /locus_tag="PLH85_03250"
     CDS             45649..>57638
                     /locus_tag="PLH85_03250"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNB63603.1"
                     /translation="MNRIYRSIWNEVSRSFVAVAETVRSQGKPASSSVVAAAECVAPS
                     ASGDPTCSSRPGSRTLRSALRPLALEQRFMFDGAAVATVDSHAHDSTAAEAAAAAEAL
                     AAQAAQAATPARESDKASPADGPTLIRAVDPALNGGRREVAFIDSQVVDYQSLVSGIP
                     QGIEIQLVDGTHNGLEQIAQWAASHHDYDAIHILGHGSSGALTLGTATLDGDTFGAYQ
                     DTLGTIGGALKAHGDILLYGCNVAATTAGTDLIQSLARATRADVAASDDATGATRLGG
                     DWVLESTVNHIETRSLQFAQYDGLLANPDVWQWGAPATVVSGGQVRSLPTDTFYIRPN
                     GTTSTVLKLPSNQYFGYYEINSGGPGYFEYSTNNGSSWTILANHWSAFAPPNALVRFH
                     ASGGSVSEVDFGAVTCLDATNTTTAANSGFYNGGGYKVRIDTAPTDIASNNSSGLLVS
                     DTPLGSDIATLTPTDTGTSLGGYWAIDSQTVSGLFGLSFNAATGNTAHLVLASTSNLP
                     SEGQTASVTVHFYDALQTDVTGAPIGGQGVTKTYTFTIKGPSHDLNFGNDIAVNPLAG
                     DTVNQSEPAITTLSNGNFVVTWADISNGGIYSRVFDSAGTAQGAPITIDAGSSATQPS
                     ITALAGGKFAVVYGDATTYDIRYKIIDGATGSASSNGLIEAYAVDGGGNQTGGWDGYD
                     PIGVAASADGSQFSVTWNLGNYTTVKTAVLSDSGTPNGAIVDLSGVTAGFAPVTAVLA
                     NGDVVTASIDGNAWAGFNLTVGGALVSGGPVGDNGTGVSIATLDGGGFVVAWSKGDGS
                     HVYAQTFSNAGTPTSSILQADSQGTAMSAARVSALGGGGFVVVWQSDGLDGSGTSIQG
                     RRFDAAGSAVDAQEFQVNEYRYGDQSTPVVTPLDNGAFATAWVDSATSGKTTGSDIEA
                     RVLSTAPSITSATYDAATGVLNVTGDNIPANASGADIVAALFTLGGEGSATYTLTDTA
                     DVERTSATQFVLTLSATDRSRINQILNRNGTSASDGSSYNLAAADNWAAGATPSANIA
                     DTTGNAITVSNVALPAITSATYDAGTGVLTVTGSGLTHHAGSANDIDVTRLTLTGAGG
                     ATYTLTASTSDVDITSDTSFTLTLGSTDKTAVDSLLTANGTQVGGVTYNLAAADNWER
                     GADSSLNIADTVNGVLVSGLNPAPTITPPADINLADTGATDTFAVQTGTLSATDADGI
                     AAFGIQSPTSTNAGFTHGGITYDVARSGTYGTLYLVSTGADAGRYAYVPDAGAINGLT
                     AGATPTDVFTVTATDANASPATGTATLTVHLTGANDAPTLSATGLSPTFTENGSAVTL
                     FSGASASAAESGQNLNQLILTVSNVADGSSELLQVDGSTLALTHGNSLTTASHGVSAS
                     VSLSVGVATVTLDKAGGLSTSVMQSLIDGLGYRNTSESPSTSNRVVTLTSLRDTGGTA
                     NGGQDTTVLAIASTVTVVSVNDAPTDITLSASSLNLSAATASAPVATLGVADLDTSDT
                     HSYALVSGSGDADNGRFVLSGNTLQVGGAALSAGTYSLRLQVTDNGGLSYTKAVTVTV
                     VDDVAPTLDAPGSTPAGDASGVAPSANLQLHFDEAIAFGTSGTITLRNVTTGTVVQTW
                     NVATPGDLGSGNGQIAISGSTLTLNPTATLDEASRYAVRIAPTALRDTAGNAFAGIAD
                     DSTYRITTGVTDSAPPALLAIQRASPSTELTRSGSVSFTLRFNEAVTNADLADFALST
                     SGTATGTLGSVTGNGTDTLTVTVTGVSGTGTLGLSLGGGHTITDIAGNALPGGNPADT
                     ELYHVDRDAPGLVSINRVTDDDVLASGTAQFIVVFREPVTQVDVGDFNLLTSGSVTGQ
                     ITAVAGNGSPAITVSIGNISGNGTLGIELASGATVTDVAGNALLSATPLAGIAERYQV
                     DRAAPTALGITRVGTERTRSGSVSFNVVFSEPVSHLDATDFTLVTSGTAAGTVTGVSG
                     SGSSYTVTVGSLTGSGTVGLAFAGPQDVADAAGLAFGGTAPALNEAFVLDNTPPTVTA
                     IDRGGVNQIAANTPSDAVFTVVFSEPVSNLSAADFTVTGSAGHGSISAVDSADGKVFH
                     VTVTGVNGTVGQTLGLSYSGSVDDAVGLAGTSPFSAGQVYTVGGVLLNEGAIDQATLD
                     ALAGVNRAGTERLVTASAPVTQVVILDSRVPGLAGQLSALPAGTEVWLLDAGTSATAQ
                     ISQILAHYSGLSAIHLISHGSSGAIYLGAETLSDATLAQYASALAAWGAALSPSGDLL
                     VYGCDVAQGAVGQRFVAALAAATGADVAASTDLTGAPFLGGNWVLEDTVGTIDTGTLQ
                     ADGYYGTLSPVTFDFTPSNGVDTGTSGYVKQLVSGVKVTGTIYTTSAGTTITGSGFGP
                     TVSSNRFTDTTNSGRMMQFTFSTPSDVTSLVVGYSANSGTISGFTVKGYQSDSTVVTL
                     NVTPTFNVGYTVSLTGANDWSNLTKLQVIANGGGSVTFFADTIVATPSNPAPTDIQLS
                     SVNAPANLAGARIANIRASDSTGLISTPSSYSSPNDIFFSDTETYTEVADPSDLFTVD
                     STGLLSFSAGKSLGNGATASITLRATDSAGNTFDKAFTITGSTYNTLTVSPSQLQNFT
                     YGGAQIGTNYASTANWTGSWGNSDAFYVLAPQGAGADVFDNDWSDSLVNTIDYGALST
                     NGAYDSLAFQAGASAGSDSFAIGSQYYVVTVGTPNAPPVVDLNGTTGSGASAAFVEAT
                     HQGTGSTSGVVLVPAALLSDSDGTVNTVVLSLANSQSDSGERLYLSGSFSGVTYSLDS
                     STQITLTRTGTTADLQSALRAVRYQNNSDNPNVTTRSITVTATDNSGGSSVETVTVSM
                     TRSNDAPTDISLSSATVNQSGGTNAVVGTLSTTDVDSGSFSYSLVSGTGDTDNSLFNI
                     ASGSLRANDAAALAAGSYSVRVQTSDGSGGTYDKIFTITVADNVAPVFDVAPATANVT
                     TDGFDITASLNEAGTVLYVIVPRNAAAPSVAQVLAGQDASGSAALGSGSQSVPSAPYS
                     ATVPVSGLSLGTDYDVYVVARDSASPANAQAAAVKLSVSTLSNTAPALGTPTVIALTD
                     TAAADTFSHPGGQLSATDTDGIARFGIQSPTSTSAGFTDNGITYDIARTGTYGTLYVV
                     STGPDMGRYVYVPDATAINAVAGGATPSDSFTVTATDANASPATGTATLTVNISGAND
                     TPAPPTLNGGSAINANESATLAAATLGTLAATDAENGTLTYTLVAGSGDADNARFSIS
                     GTSLRVNATPLAAGTYSLRVRVTDNGSPAQSAEQVFSVQVSDDVAPTLQPAGSTPQNG
                     ATGIAPTAQLEMAFSENVHAGSGTVTLVDVTTGAVVETFDAATGIGSHGGTLSFSGTA
                     LVVTPGSALDQAHQFALQVPGTAVIDGSGNAYAGTSGNTDYHFTTGALDTTAPRVTLV
                     DIADPVQPNAGTVSIRFSEAVQNVGISNFVLTRDGVPVDLSGLSVGGSGATYTLDLSS
                     VTGTAGTYVLTLAPGSIQDSSGNALAAGASDSFVVDTTAPAIAAITRASATPTQGGSL
                     DFTVAFSEAVSGVDVADFQLSGTATAGAGIASVTALSDSVYRVTVTGANGNGTLGLDL
                     RPSATGIQDSAGNAIGGGATGQRYTLDNTAPGVTSIRRSAAELSAGGALSFTVSFSEA
                     VTGVDASDFTVDKAAGVNAQDADISVSGSGNTYTVTVASVGGNGLFSVDLKASGTGIA
                     DRTGNALVGGYTAGDTYLLDSTAPIVAPSQAFSIPENMAAGFIIGQVRASDANRLASF
                     RIASGDPNGYFTIDSDGVVRLSAAGAAPNAPSRDYETAPNLFTLDIIATDAAGLDSAT
                     QSITIQVLDAMENAGAPVFTTPTPVSLNDTRSYDTLTPASGTLQATDTDGIARYGIDT
                     GTSGGSTVIGGVSYDVSRTGLYGTLYLNSSTGAYVYVPDAAAINALRNATSESFTLTA
                     TDANATPLSSSATLTIDITATNDDPVLDVPVPPQTFAGPGPWTFTVPGGSVSDPEGDS
                     LTWSATLA"
BASE COUNT        10156 a        20797 c        17765 g         8720 t
ORIGIN      
        1 gcattataaa gaactattca acgacgtcaa caacattgga aaaataaatc agcgctccgc
       61 gaacaaggta ccgaagcggg atttcatcca gccctgaaaa gtcggggccg gcatcgggca
      121 tccatacagg aagccctgca tctcttcgca ccccatcgac tccagcagtt ccgcctgact
      181 ctcggtctcc acgccctccg ctgtcacttt caagcccaag ctttcggcca gcttgacgat
      241 catccgggcg atgcaacctt cagccgttga gggcgtcagc tccctcacaa acgcgcgatc
      301 gattttcacc cggtcaaaag gcaagcggtt gaggctacca agtgacgaaa aaccgcaccc
      361 gaagtcgtcg acggccacac tgaagcccag cgctttgagc tccctcagtt gccgggccac
      421 ggattcgggg tcatccacag cgacggactc cgtgatttcc agttccaggc gcgaaggatc
      481 gatcccggtt tcgtggatct gagcaagcac cttcgatacg aagtccggct cacggaactg
      541 ggcagccgag atgttgatac ccacccggat cgggggcagc ccctctgcgt cccagcggac
      601 aagctgacgg caagcctcgg cgagcaccca gtccccgatt tcgacgatca gtccggtgta
      661 ttcagccagt gggatgaaca cgtcggggga gaccatctcg ccatccggct tgaaccaccg
      721 aatcagggcc tcgcagccga ccgccatctt gtcgcccagg cgcacctgcg gctggtagaa
      781 caactcgagt ccctggccaa gatgaagcgc cgaccgcaat tcgttcaaca gctgtacccg
      841 gctctgtgtc cgttcctcca tctcgctggc atagaagctg cagtgctgat cgccttgctg
      901 cttggcctga gccagtgcca gattggcgcg tttgaggagt tcaaccgcac cgccggccac
      961 aggcacgacg tcgacgaatc ccgcgcgggc ccgcatcggg actgcatgtc cgcgcacacc
     1021 gaatggcgtc gcgaagacgt cgagcactgc cgaggcctct tcatgccccg tgcgccaaca
     1081 cacgccaaac gcgtcgcctg ccagacgcga caaaaaggcc tcaggaccga gccgatgccc
     1141 gagtcggcgc gtcacgtcaa ccaacagcgc gtcaccactg cgatgcccca gcgcgttgtt
     1201 cagttcagag aaacgaacga tgtcgatgat gcccaggacc cagccaccgc catcgaggcg
     1261 tgccagcgcg gcatcgagac gatcaagaaa acaggtccga ttgggcaggc gggtgagttg
     1321 atcgaaatag gcaaagaagt tcagccgctc aaggaggaga acgttctcga acgcaacccg
     1381 caggtgcgcg gccaggacgc cgaccagatg ccgcaacatc tcgtcagcag ggcgatccag
     1441 atctacaaga acgcaggctt ccttgccata acgactgccc aggaagaggc acatcgtccg
     1501 gtcgccgaac acagatgcct gctgggcacg cgcgcgacgt atcgactcca cccacgccgc
     1561 gtctctcagc tccgacagca aggccccctc ctcgcaggaa tagctgccgc gtccagcaag
     1621 gatgcccaag cggtcctcaa gtgtcccgcc gtcgccggtc ggaccgcaaa acgccgcctg
     1681 aatcggacgc cccaccaacg actcaatgcg gcccagcgca acactggcga actccgcgac
     1741 agaacgggga atctcccctt gcacgccgga aagcgcaatc tcccgcatgc cccgcattcc
     1801 gaccgacagg cggcggatct gttcatacgc cctgatggct gccgtcagcg tcgtcagcaa
     1861 gcgcgtgtgg gtcagttcag ccttcgtccg gtagtcgttg atgtcgtact cgcggatcac
     1921 cttcatttcc ggcgcatagc caggttgacc ggtgcgaagc acgatccgca cctcgctgcg
     1981 ccccaatgtc tcgcgaatgg tgtgcaccag ggcaagaccg gcatccggac tttccatcac
     2041 cacatcgagg aggatgaccg caatatcgtc atccccagcg atccgcgccc gggcctcggc
     2101 ggccgacgtc gcatgcagca acgcaatccg gcgcccgcag acgatggcat cacgcagcgc
     2161 gaattcggtt gcctgatgca cctctgcatc gtcgtcgacc accagcacgg tccaggcatc
     2221 tgccattccc ggcacagcgg cgctcgcgtc ctcgtcgagg agttcgagta cgtcgtcggg
     2281 cgaaggaatc gtggcggtca ttaaacggaa tccgggtcga aaatcgagcg caacactgcg
     2341 cttcacggag actaacggcc ccggatgaat gggctttagc gtccgcagcc cttcctccca
     2401 cgaacacacc gattcggcgc acaatgaacc ttcggcgaca ccatgccaat cgcacgatca
     2461 caacaagacc ggagaccgcc atgaacacga ccgaacgcct ggccagcctc gacatgacct
     2521 ggcttcagct cgaccgtgag accaatcgcc tccacgtcac gggcgtgctg gtgttcgata
     2581 cgcgcctgcg tcgagaccgc ctcgaagaca tcctgcgccg ccgcttgctg cgcctcgatc
     2641 gctttggtca gcatgtcatc cgcgatacgg tcggcgccat ctgggaatcg ggacgggtgg
     2701 acctggacta ccatgtgcag accttcgccc tccccgacgc agccgatccc cgtcacgcac
     2761 tgcaggcgct cgtgggccgg ctcgcgagca caccgctcga ccccggtcgt cccctctggc
     2821 agcttcatct ggtcgaaaaa taccggcatg gcagcgccgt ggtggtgcgc gtccatcaat
     2881 gcatcgctga cagcatcgcc cttataggcg tcgtccagtc cctcacggac gatcacccca
     2941 ccacgccgga agacgccata ccgccggccg gccagtttga cgccagcttt ttcgaacacc
     3001 tctacgcgcc tctcacccag gccatgatcg aagggatcaa gctctcgggc gcggcatggg
     3061 ccaagtactg gagtctcctt ttctatcccc agcaggtctt cgactacgcc cgtaccaacg
     3121 cggcactgac catggaagtc ggcaagctta tgggcatgcg tgacgactcc cgtacctgcc
     3181 tgaagggcac ccctcagggc accaagctcg caagttggag cgcaccgctc gcagcgtcca
     3241 gcgtccaggc cgccgcccgt acccatcgcg ccaccaacga tgccaccctg ctcgcctgtc
     3301 tggcgggagc cctcaggcgc tttttcgttc gccagggtga cggcgccgag ggggttgaat
     3361 tacgtgccct cgtccccgtc aatctgcgtg gcgagaaggc ccgggacccc ctgggcaacc
     3421 gcggcggcat cctgccgctg gccctgcccg taggcatcga tgatccggtc acccgcctgg
     3481 cagagatcca gcgccgactg gaggatttcg aagacgccta tcgggcccat gacaccctgg
     3541 gcctcttcaa cctgatcggt cagacccccc gcgccgtcca ggcccaggcc ctcagactgg
     3601 tctctgccaa ggccagcgcc ctgctctgcc acgtgccggc cgcttccgag gcgcgctacg
     3661 tggatggcgc ccgcatcagc gagcagatgt tctggagccc ggtcaccggc gacatggggc
     3721 tgtccatgag cattctcagc tacgcagggc attatcaggt cggcctgatg gttgacgcat
     3781 caatggtgcc cgatcccgag acgatcaccg gacagctggg ggaggaactc cgtttgctcc
     3841 gcaatgcgcc cgccagcagc cgcaaacctc gcagccgcaa ggccgccagc acaagctgag
     3901 cggtctcgtc agacggagcg gggatcgaag gcgtcgcgga ccacgtcggc aaacaggttg
     3961 gcggccagta ccagcacgaa catgaacacg aaggccgcag cgaggctcca ccacaccatc
     4021 ggctcccgtg ccagttccat gcgtgcggca ttgatcatgg ttccgaaact caccgtgttc
     4081 ggatcgacgc cgatgcccac gtaggacagc acggcttccg ccagcaccaa tcctgaaaaa
     4141 tccatcacca cggcgatcag caccaagtgc atgaggttgg gcagcacgtg gcggagcaga
     4201 atgcgtgccg tagacacgcc aaaggcacgc gccgcttgta cgtactccag ttcacgcagc
     4261 ttgagcgtct cgcctcgcag gagccggcac aggctggtcc agctggtcat gccgagaatg
     4321 aagcacagtg ccaacaggcg cgcgtcggcc cggtccgcgg ccgtatcgaa ccagtgcgga
     4381 tgggtgtcga tcaccacctg catcatcaga acggccgcgg caatcaggag cacaccgggt
     4441 atcgaattga gcaccgtgta gacgtactgg atcagatcat ccacccagcc ccccagatag
     4501 cccgccagaa tgccgagcgc gatggccagg ggaagggtca gcaaggtcgt cagggtgccg
     4561 atcacgagcg ccgtgcggat gctcttgagc gtcagataga gcacgtcctg ccctaccttg
     4621 tccgtcccga agacgtggta ctccgcagcc agcatcagcg ccggtccggc aatcgccagc
     4681 acccccagga gggtcagcag aagcgcccgc caggcgagcc cgcccttgcc gcgggacagc
     4741 gcccgccacg ccgcgccgac aggcacccca cggctgcgcg cgacccccag cacgaccacc
     4801 gcagccacgc ctgcccacac gcccaatccg gcggcgagcc ccagcccgcc tcgcccgatg
     4861 acatcgccaa cgtggtctga ttcgggatag cgcagatcct ttcccgcatg ccgaagtcgg
     4921 gggaattccc gactctggct gccatcgggc cgttcgaccg tctcgcgggc aaagaggtgg
     4981 agggcgagcg gcgcagaata ggttttttcg gtccgtgccc tcaggggcgc cgccaacgcg
     5041 tccagcgcac tgcgcacctc ggtggataag ggccccttct cgccgggcgc cgcgccagcc
     5101 ggcggcgcga gccgctcccg gtaatggaga ctgtccagcc cgcccaccag cacgaagcac
     5161 agcagcacgc ccaaggacgc catgcccaca gcgctttcgc cgacccggcg ccaggcgtcc
     5221 ctcaagggct cgctgcgccg ggctcgccag atgacgagtg cgaccagtgc gagcaacgcg
     5281 aagatcagga catcggtccc caggagcaca ggctggacgc ccctcattcc agcctcaccc
     5341 gcgggtcggc cagcgtatag gacagatccg tcaaaatcag gccgatgatg tagagcaccg
     5401 atccgatgaa gaccatggcc cgcaccaccg ggaaatcctg ggcattgatc gcgtcgatgg
     5461 tgtagctgcc cagaccggga atgccgaaga aggattcggt gatcaggctg cccatgaaga
     5521 gggacgggat caccaccacc acacctgtga ggatcgggat catcccgttc ttgagtacat
     5581 gaccaaacag ggcctgcaat tccgaaagcc cctttgcccg ggcggtgcgc acgtaatcac
     5641 ggtctgcttc ctcgagaaag acggtgcgat accagcgcgc gctgccgccg atgccggcaa
     5701 tgacaccgat cgccacgggc aggatcagga aacgcgaagc gtccagcccc tcgccgtagc
     5761 cggagatggg caccagatgc aagatcttgg acaccagcca ctgaccgccg atgatgtaga
     5821 acaaacccga gatcgacatc gccgccacgc acagcaccac gccggtaaag tccagatagg
     5881 tgccgcggaa gaacaccagc agcagcgcca gcgacaccgt cacgaacagg cccaggatga
     5941 aggtcggcag cgccaccgcc agggacggcc ccatgcggct gcggatctcc tgggcgatgt
     6001 cacgtccgtc atcggcgcgg ccgaagtcga agacgaacat gcgcaccgac ttgcggaaga
     6061 agatcgtgtc ggtcagggca tcgagcccct gcgccgccgt gttgtacatc agcggcttgt
     6121 cgtaaccccg ctccaccttc cagcgctcga tggcctcggg cgtcacccgc ttgacgccca
     6181 gttgcatgcg cgccatgtca tccggcgtat tcaccacgaa gaacagggcg aaggtcagca
     6241 gattcacccc gatgaggatc agcggggcgt acaacagacg gcgcaggatg taagcgagca
     6301 tggggcgcaa tgctacccgg ccggcgggcc gaacagaagc ccggacatcg tctggcgaat
     6361 cccgtcagcg cttgccgagg atgcccttca ccgcgtcctg cagatcggcc ggcagcagca
     6421 ccacgcgggc attctccgac gcccccaggc gctccagcga ggcgatgtac ttttcgccca
     6481 gcaggaagag catcggcgct gtctcggtcc ccaccgccgc ggtcacccgg cggatcgcct
     6541 cggccgacgc ctccgccagc atcacctggg cattggcatc gcgcttggag gactccaggc
     6601 gcgcctccgc ttcgaggatg gccgcctgct tggcgccttc cgcccgcgtc accacggcct
     6661 tgcgttcccg ctcggcggag gcctgctgct ccatggcccg ctgcatggat tcggacggct
     6721 tgatgtcctg gatctccacc gacttcaccg tcaggcccca atccaccgcc tcgtcggcaa
     6781 tgctctcgcg caggcgcgcc ttgatcttgt cacggctcga cagggcctcg tccagttcca
     6841 tctcgccgat gatggagcgc agcgtggtca tgatcatgtt gcggatcgcc tctgagaaat
     6901 ccgtcacgcc atacaccgcc ttgaccggat ccgtcacctt caggaaggcg atggcgttgg
     6961 tcaggatcac cgcattgtcg cgggtgatga cctcctgctc ctgcacgtcg aggatgatgt
     7021 ccttggtcac catccggtac gccacccggt caaggtaggg aatgatcagg ttgagccccg
     7081 gccggagcgt gccgaaatac ttacccagcc gttccacgat ccactcttcg ccctggggca
     7141 cgatgcgcac gcccttcgcc agcgtcacca gcgcaaagac caggactgcc agaaccacca
     7201 ccatgccgtt gctcatgcca cgctcctcac acctttgaca ccttcaggaa actgccttcc
     7261 accgccacca ccttgacccg ctcaccggct gcgatggcct cgtccgcgcg acacacccat
     7321 tcgtcggccc ccagcaccgg cttctggaag cgcacgcgcc cccgctcgaa gggcgccacc
     7381 gcctgggaca ggaggccgat ctcgcccagc acgtgagcgt ccgactggcc aatgagggtc
     7441 ttgaactcac cgcgcctgaa gaccttgaac cacaggaaga ccatggccag cgaagcggcg
     7501 acccacaggg cgatctgcgc cgtgccgccc agaggcagga ccagcagcgc caacccgacc
     7561 agcagcccac ccagcccgaa ccagatcagg aagaaggccg gaaccgccag ctcggcgatc
     7621 accagggcga tgcccaggac cagccagtgc caccactcga agaccatcgg acgcctccca
     7681 ggacctgaat aacccgtcgc tgtgacacgg tcatgaacga ctcgttcccc gggcgccagt
     7741 gccaccagag cagggacgat ccggcccgga tagcgatggc ccccaccctg tacccacgca
     7801 cggcgaatac cccttctcag ccccgcgccg ccagagctgc gacgcggccc tcctccgcgc
     7861 cgctcacccg cacccgcttg gcccgcgacg tctcgccgga taccagcacc acggccgatt
     7921 tcggcacacg gagcatctcc gcgagatacg ccaccagggc cgcgttggcc ttgccgtcca
     7981 ctggcggcgc ggccagacgg atcttcagcg cctcgccatg cagaccggcg cactcggtct
     8041 tctttgcgcc aggctgaatg tgaagattca ggacaaggcc accgtcggcg gtatggctga
     8101 accagctcac ggctgcgtca agccatgccc agcagcgaga aacgcagaaa cgccagcgcg
     8161 ctgagcgcaa tctggagcac gaagagcagc acgatcgggc tcagatccac accgccgatg
     8221 agcgggacga gacgccggaa cggccgcagg aaagggtcgg ccagcgcata caccacgccc
     8281 gccagcggcg catggggatt tacccacgag aagatcgcgg acatcagcac cacgcccatc
     8341 gtcaggtaca gcgccacctt cacggtctcc agcaggccca cgccccacat caccgccagc
     8401 aggctcagcg gatcggcgcg accgatcacg ccatgcaccc agagggtcac aaagaccacc
     8461 agcgtctgca caacccaggc cggcagcagg ctggccatgt ccagcccgaa cagaccgggc
     8521 accagcctgc gcaacggaat caccagccag tcggtggtgg ccagcacgaa ctggccgagg
     8581 ggattgcgga aggacacccg ctgccactgc atgacgaagc gcgccaacag cacgcccgcg
     8641 agcaggctgc cgagcgtatc cacgatggtc aggatcaggc tgccgaacat ggaatgtcct
     8701 cctcagtcgc ggccgagctg ttcgcccagt tcccgcccgc gggcctcggc ggcagccacc
     8761 gcacgggcga tgatggccgg caggccgtcg gcggcaaaag acttgagcgc cgcctcggtg
     8821 gtgccgccct tggatgtcac ccgctcgcgc agcacggacg gcggttcgtc gctacccgcc
     8881 gccagcttcg cggcgccgag taccgtatcg agggccagct tccgtgcagt ggccgcgtcg
     8941 aatcccaggg ccgtgcccgc cgcctccagg cattcgatga aatggaagac gtaggccgga
     9001 ccgctgccag acaccgccgt caccgcgtcg atctgtgcct cgtccggcac ccacacggtg
     9061 ctgcccaccg ccgccagcac ctgctccgca gcggcccggc cttccgcaga caccgaggca
     9121 tcggcataaa gccccgtaac gcccgccccg atcagcgccg gtgtgttggg catggcgcgc
     9181 accagggtgc ggtaaccgcc cagccagcgg ccgatgtcgg ccagacgcag cccggcggcg
     9241 atgctcacca ccacctgggc tcccagacgg ccggcgaagg gcgcgacggc cgcccgcagc
     9301 tgctggggct tgacggccag caccagcaca tcgcaggcca gcgccgtgtc atcgagcgcg
     9361 tcgaccgccc gcacaccgaa ctgggtcgcc agcttttcgc gaccctcggc cgagagttcg
     9421 acgacctgca cgccggtggc gtcaaagccc tgtttgacca gaccgccgat cagggctgtg
     9481 gccatgttgc cgccgcccag gaaagtaatc ttcatctgtt cgctttcgtg gagtggatga
     9541 aatgtgctca gttgcgggca ccgaagatgg ccgtgccgac acgcacgagg gtggcgcctt
     9601 cggcgatggc cgcctccaga tcggcagaca tgcccatcga gagcgtgtcg aggccgaatc
     9661 cggcggcgtt gagttcgtcg cgcaatgccc gcacgctggc gaagcgccgg cgttgcaggg
     9721 ccacgtcgtc ggtcggctcg ggaatcgcca tcaggccacg cagacgcagg cgcggcaatg
     9781 ccgccacggc cgcggcgaga tcggccacct cgcccggcgc cacgccgctc ttgctggctt
     9841 caccgctcac gttcacctga aggcacacgt tgaggggcgg cagatgcacg tcgcgctggg
     9901 ccgacaggcg ttcggcgatg cgcagccggt cgatggaatg gacccacacg aagcggttgg
     9961 ccaccgaccg cgtcttgttg ctctggagcg gaccgatgaa atgccattcc aggtccggat
    10021 ccgccagcgc gtccactttg gcgcagcctt cctggacgta gttctcgcca aacgcccgct
    10081 gccccgcggc ggcggcggcc cgcacgcact ctgccggcca ggtcttgctg accgccagca
    10141 gcgtcacccc gtgaggatcg cgtcccaccg cctgcgctgc ggtcgaaatc cggtcacgaa
    10201 ccttttgcag gccgacggaa atgcctgtca tataatcgtt caagcttaaa tccggagagg
    10261 ctcgagccgt agattgcaag gccgccgagt atagcactgc ccaccggcga ccctttttgc
    10321 cgcgccttcg caccgaccgc ccttcttgag agtttccgac catggatatc accgaactgc
    10381 tggccttcac ggtcaagaac aaagcgtccg acctgcacct gtcagccggg ttgcccccca
    10441 tgatccgggt gcacggcgac gtgcgccgca tcaacctgcc cgcgctggtg cagaaggatg
    10501 tgcacggcat ggtgtacgac atcatgaacg acacccagcg caagcagtac gaggaacacc
    10561 tggaaaccga cttctccttc gagattccca acctcgcccg cttccgggtc aacgccttca
    10621 accagaaccg gggcgccgcg gcggtgttcc gggtgattcc gtccaaggtc ctcaccctgg
    10681 aagaactcaa ctgcccgaag atcttcaagg acatctgcaa ccagccccgc ggcatcgtgc
    10741 tggtcaccgg ccccaccggc tcgggcaagt ccaccacgct ggcggcgatg atcgactaca
    10801 tcaacgaaaa cgagtacggc cacatcctca ccgtggaaga cccgatcgaa ttcgtgcatg
    10861 aatccaagcg ctgcctcatc aaccagcggg aagtggcgcg cgacacgctg tccttcaacg
    10921 ccgccctgcg ctcggcattg cgggaagacc cggacaagat cctggtgggc gaactgcgcg
    10981 acctggaaac catccgcctc gccctcaccg cagccgaaac cggccacctg gtgttcggca
    11041 ccctgcacac ctcgtcggcc gccaagacca tcgaccggat cgtggacgtc ttccccgcgg
    11101 ccgaaaagga catggtgcgg gcgatgctgt ccgaatccct gcgcgcggtc atctcccaga
    11161 ccctgctcaa gaccaaggac ggttcggggc gcgtggccgc ccacgagatc atgatcggca
    11221 ccccggccat ccgcaacctg atccgcgaga acaaggtcgc ccagatgtat tcggccatcc
    11281 agactggtca gaacctgggc atgcagaccc ttgaccagag cctcacggac ctgatccggc
    11341 gcaaccagat ctcggcggtg gaagcccgcg tctatgccgc caacaaggat tccttcaccg
    11401 ggtgactcat caaaatggtg gagtgattta caattcttaa cagccgacgc ccggatcggc
    11461 cttcacccga caggaatgcg ccatggaacg cgaccaggcc ctcaagttca tgcacgatct
    11521 gctcaagctg atgatgcaga agaacgggtc cgacctcttc atcaccaccg gcttcccgcc
    11581 cgccatcaag gtggacggca agatcacgcc ccagtccaac caggtgctca gcgcagccca
    11641 cacggccgaa ctggcgcggg tcatcatgaa cgaccgccag gccgcggaat tcgaagccac
    11701 caaagagtgc aacttcgcca tctcgccggc cggcatcggc cgcttccggg ctaacgcgtt
    11761 catccagcaa ggccaggtcg gcatggtgct gcgaacgatt ccccagcgca ttcccaactt
    11821 caccgaactc ggcctgccgc ccgtgctcaa ggacattgcc atgtccaagc gcgggctggt
    11881 ggtctttgtg ggcggtaccg gtaccggcaa gaccacctca ctcgccgcaa tggtggatta
    11941 ccgcaacgag aagagttacg gccacatcat caccatcgag gatccggtcg agttcgtcca
    12001 tccccacaag aactgcatcg tcacccagcg ggaagtgggc atcgataccg actcctggga
    12061 agcagccctc aagaacacct tgcggcaggc ccccgacgtc atcctgatgg gcgagatccg
    12121 cgaccgggag accatggact acgccatcgc cttcgcggaa accggtcacc tgtgcctggc
    12181 caccctgcac gccaacagcg ccaaccaggc catcgaccgg atcatcaact tcttcccgga
    12241 agagcgtcgc gcccagttgc tgatggatct ctccctcaac ctgcgcgccc tcatctccca
    12301 gcgcctgctg cccatgtgcg accgcaaggg tcgggtaccg gcagtggagg tcatgctcaa
    12361 ctcgccgctg atctcggatc tgatcttcaa gggcgagatt cccggcatca aggacgtgat
    12421 gaagcgctcg gccgaactgg gcatgcagac cttcgaccag agcctgttcg acctctacga
    12481 acgcggcctc atcacctacg aggacgccct gcgcaacgcc gactccatga acgacctgcg
    12541 tctgcagatc aagctcaaga gccggcgggg agacaacgac atgttgtccg gcatccagaa
    12601 tctcgacatc gtctgaagcg ccgaacaaag cggctaagcg cctcgatctc gccgcggtga
    12661 tactcgccgt accccatgta cggccgcgtt tctcacgacg accccgacgc gctcgcgacg
    12721 ctttctgcca cgcttcagcc gccggtcaat ctgcgtccgc ggcgccgcct tcccccttcg
    12781 gcttgcgcgc cgaaccggcc accatcgcaa attcgtacaa ccggcattcg atggcgccgt
    12841 tgaagagcgg cgtgcgcttg ctggctttga gacggacgcc cttgggcagc tccgggtcgc
    12901 cgctgaagaa gtagcagcgc cagcccgccc agtgggcctt gagggcgctg cccaggcggg
    12961 gatagaagtc gcgcagttcc gacatgtcag acaggcgcac gccgtagggc ggattggcga
    13021 ccagcacgcc gctcgccgcg ggcgccgcca gttccagcat gtcggtgcgc gagaccgtca
    13081 cgcagccgtc caggcccgcg ctgcgcagat tgacgatgct cttgcgttcc tgggcctcga
    13141 ggatgtcgga gccatggatg ggcaaggggc gcggcgcctg gcgccgggcc tcggcggcct
    13201 ggcggatcgg caaccactgg tcgcgctcga agttcttgaa cttctcgaag ccaaagcgcc
    13261 gccccaagcc cggcgccaca tccagagcca tctgggccgc ctcgatgagg aaggtgccgc
    13321 tgccgcacat gggatccacc agcgcctcgg atcccggttg ccagccggtc aggcgcagga
    13381 tgcccgccgc caggttctcc ttcagcggtg cctcgactgc ggccggcttg aacccgcgtt
    13441 tgtaaagcgg ctctccggag gtatccagat acaaggtgac ggtatcggcc gacagaaagg
    13501 cgtggatgcg cacgtccggc gccttggtgt ccacgttggg ccgtcgtccg gccacgctgc
    13561 ggaaatgatc gcagaccgca tccttgatgc gcagggtggt gaattccagg ctgcgcaagg
    13621 gcgacttgtg cgccgtgacg aagacccgca gggtctgatc cgccgtaaac cagcgggccc
    13681 aggtgggcgc gtaggcaatg cgatagacgt cgtcctcgct gcggtagcgc ccggaggcca
    13741 cctgcagcat cacgcgggtg gccaggcggc tttccaggtt ggcgcgatag cacaccgccc
    13801 agtcgccgga gaactggacg ccgccgggca gcgccttggg atcgacggcg ccgatactgc
    13861 gcagttcgtc gacaagaagc ggctccaggc cgcggggtac gggagcaaag aaatccaaga
    13921 gacggtaccg gaaagaacga tcagaaaggt ttgaggacca ccaggaagac caccgcaaac
    13981 agcaccagga ccggaatctc gttgaagaag cggtaccaca cgtggctgcg ggtgttgcgc
    14041 ccctcggcaa aggcccgcag gaggcggccg caatacaggt gatagacgat cagcagggtg
    14101 accagcgccg tcttggcgtg cagccagccg ccgacgaagc cgtagccgaa ccacagccac
    14161 atgccgagga gcacggccag gatgcccaga ggcgtcatga agcggtagag cttgtgctcc
    14221 atcagcgcaa tacgctcgat ggtcgccgga tcggtgacca tggcgtgatt gacgaacaga
    14281 cgtggcaggt agaacaggcc ggcaaaccag ctcgtgacga agatgatgtg aagggctttg
    14341 acgatcagca tgggaaggct caggaaacgg aatagtgggg tcaggactca ggccagacgc
    14401 ttcagggcgt ctgcgacggt gggatggatc aaacggacac gcagttcccg cttgatgcgg
    14461 tcattgcgaa tgcgtcggga ttcggacatg aacgacagag tcaggggcga aacctgtgcg
    14521 atcaactcgg cccggggcag gcgctgaggc cgcggcaagg cgaaggcgtc ggccacggcg
    14581 tcgaaatagt cgcccatccg catctggctg tcgtcgcagg cgttgtagac ccggtttgcg
    14641 cggccgcgga acaggctcaa gccgagcagc cgggccagat cgtcggcatg gatatggttg
    14701 gtgaacacat cgtcgtccgc cgccagcacc ggatcgcccc gccggatgcg ctccacgggc
    14761 aggcggtcaa ccgcatagat acccggtgcc cgcagaatgc tcaccccgca cccgctgcgc
    14821 gtcccgaagc tgcgcaggcg gcgctccgcg gcgaccctgc gcacgccccg tgacgacgcc
    14881 ggcgcaacag gacgcgtctc atccagccac gcaccgccac aattcccgta aacgcccgtc
    14941 gtacttatgt atatgagccg acgtggtaga ctggcgcgtc gcgacagcgc ggccagcagg
    15001 ctgcgggtcc gggggtcgtc ggaaccctgt ccgggcggcg gtgcgcaatg caggacggcg
    15061 tccgccacgc cggcgagccg atcaaggctc gcccgatcat ccagatccgc caccagcggc
    15121 acggcgccca gcgcccgcag cgcttcccgc tcttccgggc gccggattac ggcaaacacc
    15181 cgacagcggc gggccagcca cggaatcagc cggcgcgcca cgtcgccgct gccaacaatc
    15241 agaacccgct tcgttttcac acgactcatc cgttcattat ctcacggcca tccatcatgt
    15301 cgttccaggt cacgctccag cccggcgccc accagttcgc cgcctctgcc gatgaaaccc
    15361 tcctccagtc cgcccttgcg gcaggcttgt tgctgcccta cagctgccgt gacggtgcct
    15421 gtggtgtctg caagggcaag gttgtctcag ggcgtgtgga cctcgggcgc tattcggaat
    15481 caagcctcac agcgtccgag cgcgcccagg gcatggccct gatgtgttgc gccaggcccc
    15541 tgaccgatct cgaactggaa gtccgtcagg tcaatctggt cggcgagatc ccggtcaaga
    15601 agatgccctg ccgcgtgcag aagctgcagc gcctctgcga cgacgtgatc gtgctggatc
    15661 tgaagctccc ggccagcgaa cgattccagt tccgcgccgg gcaatacatc gacttcctcc
    15721 tggccgacgg caagcggcgc agcttctcca tcgccaacgc gccgctcgac ggcgccgacc
    15781 atctggagat ccacgtgcgc ctggtccccg gcggccagtt caccacccac gtcttcgagc
    15841 gcctcaaaga gaaggaaatc ctgcgttttg agggccccct cggcagcttc ggcctgcagc
    15901 ccggcgccgc taaacccgtc gtgctgctgg ccggcggcac cggcttcgca cccatcaaga
    15961 gcatcgtcga gcaggccatt gccgacgcct cgacacgacc gatgaccctt tactggggca
    16021 gccgcgatcg cgccggactg tatcagttcg atgaggcccg cgcctgggaa gcccgcctgc
    16081 cgggtttccg cttcgtgccg gtactctccg acgtcccggc cggcgaggcg tgggacggtc
    16141 gtcgcggtct ggcccaccac gccctgatgg aagatctgcc cgatctgtcc ggccatgagg
    16201 tgtacgcctg tggttcgccc gccatgatcg acgcagcccg cagcgatctg gtggcgcgct
    16261 gcgacctgcc gcccgacgcc ttcttcgccg acgccttcag cttctcgaac gacgcccgcg
    16321 gggcctgaca cccgcaccgc cacgaggacg cccatgagcc acaccgccct gatcacccgc
    16381 gaaccggtga tcaacaagca gtacaagatc accgccaacc ggctggtcgt ccacgccaaa
    16441 tcggtgggcg aagtcgtgca gaccctcgac ggtgtggcag acgtgtggcc cgccacccac
    16501 ccggtcttca tcagccttta ccggctggtc cccacgcccg acctgctgga gtggatcgct
    16561 ccggccaacg ccatggtgga aatccccacc caggccatcg cccatcccct gacccagcag
    16621 ctcattcccc agctacgggc ccagggcgtc agcctcaacc tcacctggta tcacccggac
    16681 gtgacgctgc cgcccgacgt ggactggcgc ttcgtgatca tggacgccca caagcatccg
    16741 gcgcccctcg atccgccagg actcagcatt gcctggggac tggaggactt ccccgccttc
    16801 cgcgccgcca tcggcagcgg ctatgacggc gcctccggct ggttcttcct caagggcctc
    16861 aacgcaggcg gcaagctggc gccatcccac gctcagatcg tgcgcctgct caatctggtg
    16921 cgccgcaatg ccgaagtgaa ggaggtggag accgttctca agcaggacgt ggcgctgtcc
    16981 tacaaactgc tgcgttacat caactcggcc ggcttcgggc tgatgtgcga gatccagtcc
    17041 ttccgtcacg ccgtcaccat cctcggctac gacaagctgc acaagtggct gtccgtgctc
    17101 ctcgtctcgg ccagccgcga tccgtcggcg cccgccctga tgcagacggc catcgcccgc
    17161 ggtcgcttca tggaacgcat cggcgaagcc ttcttcgaca ggaaccagct cgacaacctg
    17221 ttcatcaccg gcgccttctc catgctccac ctgctcctcg gcgccagcct gcactctgcg
    17281 cttgaggaga tgcacctgcc tgaacccatc gccgacgccc tgctgagcgg cgaaggtgtc
    17341 tacgccccct tcctcaagct ggcccgtgcg ctggaaggct tcgatgacaa acacctgacg
    17401 gaactcgcgg cggatctgca catctctgcc gatgtcatca atcgtgccca cctcgaagcg
    17461 ctcggcttcg ccgacagcct gcagttcggc tgataccggc ttcatttcgg ccctgcaacg
    17521 gggggccgaa caatcctttg cgaactcttc gttcgtttgc gaactgggtc acgaacgtcc
    17581 cggaacgacc gaacatttgt cacagccggt cccctcaggc ctacggttag aattggatca
    17641 agcctttcgc ggcaaccgtc caacccttat cactgccttc atgcctgaca ccagatcgct
    17701 ttccctgcgg cttcccagcc cggtctccgt atcgcggatg cctgcgctgc cggaatgcgc
    17761 cgccaccctg ctcaaggtgg tcgcggaagg cacgacgtcg agtgaagaca tctccctgcg
    17821 gatctgccgg gatccgatcc tctttctcga aatcctcaca acggcccccc tcggtcgcga
    17881 cgagcgcagc acgccgctca aggctgtcga gcggcgcatc gccgaactcg atcccggcct
    17941 gctgcaggcc tggttcctgc gccacgccgt accggcaccc ggacagcgga cctccatcgg
    18001 ctccctgcgc aacctgtccc tgcacagcca gctggtcgcc gaactggcct ttcacctggc
    18061 ccgcgagacc gcctacccga cgcccgaaga tgcgtacgtc gccgggcttc tccacgatgt
    18121 cggccagctc tggctcgccg aacatcagga aggctacgtg accatgcgtg ccgcgctgag
    18181 cgacgagaag gccctgagtg cagccgaatg ggaacgctac ggcgccgacc acagcacgat
    18241 ttcggccgaa ctggcccacc agtgcggtct gggggccctg gtcgccgacg ccatcctgct
    18301 gcaccagacc tcgcccgtgc agctgcgctc ggctcacctg ctggtccgcc tcatcgccgc
    18361 cgccgaggag ctggccagct atcgtcagac ccagtcggcc ctctccctcg atatcgccag
    18421 ccacctggca ggcctcgacg gcagcgtgct ggtcagcctg ctcgccgaaa ccggtccgca
    18481 ggtcaatgac gcagcgagcg atttcggcgt tgccatcgaa ccggcgatgc tcgccctgcc
    18541 ctgggccctc ggcccggatg tggcacctgc gcccgaagcc ggcaccacgg gcatctcccg
    18601 tgccgcactc aatgcctttg cccgtggcac ctttgccagc gcagaagaag cgcgcaatgg
    18661 tctcgcccgg gcggcgcgcc tgctcctcgg gctcgacgca cccgcactct tcgtccgcag
    18721 cgataacgga cttgctttgg tcggctgcgc cagcgcgacc catccgtccc tcgaccagat
    18781 gtgcgtcacc ctggccgatg acaccagtgc catcgccgaa gcggctcgca gcaatgcaga
    18841 gacccgttac cgctgcgacg aacgcccgcc cggccgcagc gccatcgact ggcaggtgtc
    18901 ccgctggctg ggcagcgacg ggatgctctg cgtaccctgg aagaatcatc acgacgcggg
    18961 cgtcgccgtc ttcgcctttg ccagcgcgga cagtgacccg ccagacagca cacgcgccct
    19021 gcgccgcgat ctcgtcctgt cggccaccct gcgcctctcc gaagaacgtg ccagccagca
    19081 tctgcgcgac gacctggagc agaccatctc gcggcgctat gcagaacatg cccggcgcct
    19141 cgcccacgag gccagcaacc cgctcaccgt catcaagacc tacctgggca tcatcgacga
    19201 acggatcgag gatgctgcga tccgcgaaca gctcggcgtg ctcaacgacg aactcgaccg
    19261 catcggcaac ctcgtccgtc gcgcccccga tttccgcgag cctgcggccg acggcccggc
    19321 tcaaagcggc gtcaccgaaa ttctccacga cctgcgtgcc ctttatggtg acgccctgtt
    19381 cgcgcggcgc gggatccagt tcgaagtccg caccacgccc gatctgccgt ccgcagccat
    19441 cgctccggat gccctcaagc aggtcctcgt caatctgttc aagaacgcgg ccgaagcgat
    19501 tccggaaggc ggcaagctcg tcgtcacggt cgggaccggc atcaacgcca acggcacctc
    19561 gtgcctggag atccgcgtgg tcgacaacgg accgggcctg ccccccggcc gcatcgagac
    19621 gctctttgca ccggtgcccg gcggcgccgg ccgcacggaa ggcgggatcg gcctgcccgt
    19681 cgtacgcgat ctgatcaccg cgcatggggg gaccgtcctg tgccgcagtc agctgggctc
    19741 cggcacagtg ttccagatct tcataccgct ggcgggcacc ccctgaattg atgcatcatt
    19801 gcggcgcaaa acaataattc acagggagtc catccatggt cattcccggg gagcgcccct
    19861 tcttcccgcc cgagacggga aaccgaggcg cacccgatcc tctgatccgc atacttgttg
    19921 tcgatgacca gccgtccctg cggcgttcgg tcgtcgccct gcttcaaggc gatgaccgcc
    19981 tgatcgaaga ttgcggctcc gtcgccgaaa cgctcgtgcg cctcggcgcg gccagttacg
    20041 acctcatcat cctcgactac cgcctgcccg acgccaccgg tctcgccgtc atgaactggc
    20101 tgtcgagcca tcaccgcaac gaatcggtga tcgtcatcag cggcgaggat tccatggaag
    20161 cggccattgc cgccctccgc cgcggcgccc aggacttcct gcgcaagccc tacgaccccg
    20221 accagttgcg tcgtgccgtc cagtcggtcc tcggcaaacg tcagcaggag cgcagtcacc
    20281 gcctcaccct gcagcgcctg caatcgtccg agcacatgca ccgctatctg gtggaaagct
    20341 cccccgacct gatcttcacc accaccccgg acggacgctt caactacatc aatccgcgca
    20401 tcgaatcgct gctgggctat cgccgcaaca gcctgatcgg ccaacatttc tcggagatcg
    20461 tccatcccga ggatctggag cggacccgtc agaccttcca cgagcgccgc accggccagc
    20521 gcgccgccgc caatgtggaa gtccgtctca cccgcagtcc cttcggtccg gaccaggacg
    20581 gcgaggccgg gcccctgccc tttgtgctca acgcccatgg catctaccac gacgaggacg
    20641 gcacgacgcc gggtgaatat ctgggcacct atggcgtcgc ccgccacgca ccgccgctcc
    20701 gcctgacggc actggccggc aaccctcccc tgcagcgcga cccactcacc cgactacccg
    20761 gacgcgagct cctcttcgac cggctcgacc tggccgtggc ccaggccaag cgtcgcaagg
    20821 ggcagatcgc cgtcatgcag gtggacgtgg accgcttcaa ggacgtcaat gagcgcttcg
    20881 gtcagggcga aggcgacacg ctcctgtgcg ccatcgcgcg ccgcctgcag cgctgcctgc
    20941 ggcggggcga caccttgtcg cggcagggtg gcgacgaatt tacgatcctg ctgccggacg
    21001 tccgcggcaa ggacaacgcc caggccattg ccggcaagat ccaccaggcc ttcgagcagc
    21061 ccttctctct gggcaaggac gaggtccgcc tgaactgcag tatcggcgtc gccctctacc
    21121 ccgaagacgg cgacgacgca gagtccctca cccagcacgc ggccatcgcc atgttccagg
    21181 ccaagcgcaa tggcggtgaa tcccacgaat tcttcgaacc tggcatgcat tcggcgtacc
    21241 gggcgcgtat cagcctcgag accgaattgc ggctggccat ctcgaaccgg gaactcgaac
    21301 tgttctatca gccccgtgtc agcctgtccc gcaaccaggt gaccggcatg gaggcactga
    21361 tccgctggcg gcatcgtaac cacggcctcg tcgcaccatc ccgttttctg caactggcgg
    21421 aagaagtcgg catcatgccc gacatcagcc gctgggtgct ggagaccgcg tgtgcccagc
    21481 tgtccgcctg gcgccgcctg tatcccgaac tgcgcctggc caccaacctg tccctgcgcg
    21541 acttcgaccg ggcggacctg aacgacgcca tctacaaggc cctcgcccag cacaacctgc
    21601 caccggacag cctcgaactg gagatcaccg aaaatctcct gctggaagac cacgaatttc
    21661 ttgcggccaa ggtccagggt ttgcgcgaca tgggcgtcgg tctgtccatc gacgatttcg
    21721 gcacgggtct gtcgtccctg tccttgctgc accgctttcc cgtgacccgt ctcaagatcg
    21781 accgcagctt cgtgcggaac atcaacgggc agggcagcca tcccgtgatc aacgccatca
    21841 ccggcatcgc ccggggtttt gacgtgcagg tttcggccga aggcgtcgaa cggggagacc
    21901 aggtcagcgt gctggaaacc gccggctgtg acgagatgca gggctacttc ttttccaagc
    21961 cgctcggcgc tgaagccgcc acccgcatgc tccaggattt ccgtcccgca gccatgctgc
    22021 agacagcgcc gggctgaggg cgccggctcc atcattcgtc acattcgtcc gctagagtgc
    22081 gcgcgtcacc ccgatgtgga gaccgccatg ggaaccttcc gcctcatcct cgcccacgcc
    22141 cgggcaccgc aacaatccac cgtcgtcgcc cagcatgtcg atccggtctg gctcaagcag
    22201 cgcggctacg agatcgcccg cagcttgggc gacggcagcg ccctgtgggg gcgccgcgcc
    22261 gatgccggaa acctgtcggg cctcgaactg aactgccgcg gcggtcacgc cctacgcatc
    22321 gaggcggcct gagccgaccg cctcagcggc aggatcaacg ggaaggctgg atcacgcccg
    22381 gcgcgtagcc ctgctcccgc aggcgcgact gggcagccag tgcctctcgc cggtcggcaa
    22441 atggccccac catcacccgg ctctccagct ggaccggcac gccgccacgg gccagacgcg
    22501 cccgcaggtc ctctgcagcc gcgggctcca tgaagccgcc cacctgcacc acatagcccc
    22561 gcgccggtgc tgcgcggcca tccgcgggag gacggacctc agcctgcggn nnnnnnnnnn
    22621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cctgcggcgc gacggccggc gcagacggtg
    22741 caccggcagg cgccgccgcc ggtgtagccg ccaacggcgc gacgcccccg cgaagggtgt
    22801 cttccttcag tgtcaccgac ctgctggagg ccaccgccgg cgattcaggg agtaccgtac
    22861 gggcgcccgc cccgacgaca ggtcgggtct cgtccgcagc atcatccgcc ttgcgggctg
    22921 ccttgggcgg ttccccctga tcggcagcga caggcagggg aggcgccgag gacgcctggt
    22981 ccgccgcctc gcctcctgcg cgcacgacgt ccggcggcgg ctcgctctcg cggcccgcca
    23041 ccggtgccgg accgatgggc ttggccggca ccttggcctc ctctgcttcc ggcgggcggg
    23101 aggcctcgtc gaaaacgatc agactgccca agagcaggcc gatgagcccg acggccaccg
    23161 cagcgcgggt ccacaactgc cgccgggaat catccttttc gtcctgaacc ggcgggttgt
    23221 tccgttcatc ggtcattcat gcacctcccg acgcgccagc gcacacacca gttcggcctc
    23281 ggccacggcg attccgcagc gatccgcaat ctgtgcagcc gtcatgcccc gttcggccag
    23341 caccatggct tcggaatgct ggggtgacac cggccgcacg aaatgactgc ggccgtcggc
    23401 cacctggatt tcgtccagat cacgccgcac cgacgcgatc tcgcgccgca gctgatcctg
    23461 ttcgccccgc aactgggcca catcccgtcg aagctgctgg acttccagct ggaagaactc
    23521 gatcaggtcg aaggtgggca ggctgtcggg ctgttcggca gccgaagact caccccgacc
    23581 ggtgcggggg tcggcgtagt catccaacgg ttcgatgtcg ccaacgtccg catcctcggt
    23641 cggggcaggc gtccggatcg acgccacatc ctcgccggag ggggttagag cggcgggcgc
    23701 cacaccttgc ggcgcaggcg cttccgcatc gaccaccagt gtctggggtt cttctgcgag
    23761 gggacgggcg cgatggagcg ccagacggat caggacgaag gccagataaa gggtcaggac
    23821 gccgatggcg atccagatgc cttcccgcac accgatctcg atcatcgtgg aatcctcccg
    23881 gagcaggcga cgggaatgaa cgatgccttc acgattccga cgatgatgag cctccaatgg
    23941 gcagggcggg attgacgctg cgataatagc cgtcacgccc gcccccgaca atctggagtc
    24001 ggctccccgc ctcgcctttc gccatgttct tcctgaaaaa gatcgccgcc gcgctgctcc
    24061 tgccgccctt cctgcccttt ctgctgatca tcgccggcct gctgctgctc caccgccagc
    24121 cccgcacggg ccgcctgctg gcctggtgcg gggtggtgac cggcattgcc ctgagcctgc
    24181 cggcctccgt cggtctgctc gtcacgcccc ttgaacggga cgcggccctc gatgtgcgag
    24241 acctgcctgc cgccgaggcg atcgtgatcc tggggggcgg cacccgccat tacgcgccgg
    24301 aattcggcgg acagaccgtc aatcggctca cccttgagcg tttgcgctat ggcgccctgc
    24361 tcgcccgtca ctcgcgtctg cccgttctgg tcagcggcgg cagccccacc ccgggcattg
    24421 ccgagggcat cctgatgcgg cgggtgatgg aagaggagtt cagggtgtca gtgcgctggg
    24481 tcgagagcca gtccctcgac acggccgaaa acgcggcctt cagcgtgccc gtgctgcgcg
    24541 cggcgggcgt gcgacgcgtg ctgctcgtca cccacgcagc ccacatgcgg cgggcgctgg
    24601 aagcatttga agcggccggc atgcatgcca tcccggcgcc caccgccttc ttccacggcg
    24661 ccgggccggc ggatgaagtg gtggatttcc tgccgggcat gaatgccgcc ttcgccggcg
    24721 gctatgccgc ccacgaatgg ctgggtctcg ccgcccggcg cctgacagac ctgggcggcg
    24781 gcctgatcag gcgttgaagc caggtttgcg tttttcgagg aaggcgcgga taccttcatc
    24841 gaaattgggt cgggcactgc tgcgggcgaa ggcctccgct tccagttgaa gctgcgccga
    24901 gagcgactgt tcgggcgacg ccaccagcag gcgcttcatc tcggcgtagg cttcccgcgg
    24961 gcccttcacg aagcgctgga cgagggcatc cgtggccgcc tccagctctt cgggtggcac
    25021 gacgcggttc accacaccca ggcgcagggc ctcatcggca ccgaagcggt ccgccagcag
    25081 gagcagctcg aaggccttgc gccgccccag gatgcgcggc agaaaccagg acacgccgcc
    25141 gtcaccggac agggcaatgc tggcgtaggc cgtggtgaaa taagcgtttc cggcggccac
    25201 cgccatgtca cagccgagca ccagagagag cccgaagccc gcgcaggcgc cccgcacctg
    25261 ggcgatgacg ggcaccggca aggccgccag ggcctcgacg ctgggattga tgtactgctc
    25321 gatcagcgcc cggaaggtgg cgatgcggct ttcggccgac agatgcaggt gcgtcgcgaa
    25381 atccttcaga tcgccgcctg ccatgaagtg ctcgccggcg ccggtcacca ccacggcccg
    25441 cagatcggtg cggcgttgca gctggccggt ggcttcggcc agggctttca tcatgtccac
    25501 cgacagggcg ttgagcgcag taggccggtt gagggtcagg gtggcgatgc cgtcctgcag
    25561 gtccagcgtg acgggcgagg tcatggatcg ggctccagaa ggaatgtgcg cgtattgtca
    25621 ccgcgaaacg cgccaggcgg gtagcgcccg gcgcccgtca acgcggacca tcggccacgg
    25681 cgtaacgctc gcgcatcagg cgcttgttga gcttgccgac gctggtcttc tccagggcct
    25741 ccacgaagac cacccgctcg ggcaccgcga agcgggagat ctgcccggcc tcggcgcagg
    25801 cccgcaggtg ggcgatgatc gcctccgcat ccaccgcctc gcagccggcc cggcgcacca
    25861 ccacaggcag gggacgttcg ccccagcggg cgtccggtac gcccagcacg gccacctcgg
    25921 ccacggcggg atgccgcgaa acgagggttt ccagctccag cgacgagatc cattcaccgc
    25981 cggacttgat cacgtccttg atgcggtcgg tgatctgcag ggagccggcg ggggagatgc
    26041 gcccgatgtc ctgggtgtgc agccagccgc ccgcccacag ctgggcggtg gcctccgggt
    26101 tcttcgtata gccctgggtg agccagggcg cgcgcacgac cacctcgccc tgggcgtggt
    26161 cgtcatgggg cacttctgtc ccgtcggcat ccaccacgcg cagatccacc agcggcagag
    26221 gcaggccggt acggcagcgg gcggacacgg tttgctcggg cggcaattcg agttcctccg
    26281 gcttgagctg ggccagggtg aggatcgggc aggtctcgga catgccgtag ccagcgaaca
    26341 ggtccagccc acgcgccacg gcctgggtcg ccagtccggc ggtcagggcg gaaccgccga
    26401 tcaccatctt ccagcccgac agatccatgt cccgcgccga cggtgcgaga agcagcatct
    26461 gcaggatggt gggcacgcaa tgggagaagc tcacgccctc ccgcgcgcgc aacgccagga
    26521 tctcgtccgg cacatagcgg ccgggataga cctgcttcac gcccagcagc gtcgccacgt
    26581 aaggcaagcc ccacgcatgc acgtgaaaca tgggcgtgag gggcatatac acatcgccac
    26641 ggtcgaaacg ctgcgtgcct ggcgcagagg ccagcccggc cagcccggcg agggtgtgca
    26701 gcacgagctg gcggtggctg aagtagacgc ctttgggcag gccggtggtg cccgtggtgt
    26761 agaaggtggt ggcgcgggtg tgctcgtcga aatccggaaa cgcgtggtcc gggcgccccg
    26821 tcgcgaggag cgcctcgtat tcggccagcg cgccggcggc caccggatcg cagacgccgt
    26881 cggccagcag gatcaggcgc ttcagcttgg gcaggcgccc gcggatgcct tccagcaggg
    26941 gcaggaagtc cacattgagc agcagggtct ccgcctcgca gtgatccagc gtgtaggcga
    27001 tctgctcggg cgacagacgc acattcaccg tcatcagcgt cgcccccagc atgggcaccg
    27061 cgaaataggc ttccaggtag cgatgggaat cccagtcggc caccgccacg gtgtcgccgc
    27121 gggccactcc gagccggctg agggcgctgc ccaggcgacc gatgcgtgcg gccagctccc
    27181 ggtagctgtg gcgcacctgg tcgcgataaa cgatttcctg gtccggggcg tggaccaggg
    27241 gcatgcgcag gagctgcttg atcagcagcg gataggcgta ggggccgtcg ccgatgggca
    27301 tcatgatggt ctcctcatca ttgaatggct cgccggcgct gtggccggcg gatggcacag
    27361 gggccgcctc cccggaggaa ggcggcccct gcgcgtcctg ctgcgggcgg cgcggggccg
    27421 ccgggcttac acccgctcga agattcccgc cgcccccatg ccggtgccga tgcacatgga
    27481 caccatgccg aagcgggcct ggccgcgctg catggcgctc atcagcgtgg cggtgcggat
    27541 cgcaccggtg gcgcccagag gatggccgag ggcgatggcg ccgccctggg gattcaccct
    27601 ggccgggtcc atgtcgagct gcttcatgac cgccagggcc tgggcggcga aggcttcgtt
    27661 cagttcgatc cagtccaggt cggactgggc cacccccccc agcttgaggg cgcggggaat
    27721 cgcctcgatg gggccgatgc ccatgatctc gggcggcacg ccggccacgg aatagctcac
    27781 gaagcgggcg atcggcgtca cgccgtagcg cttgacggcc gcttccgaca tgagcagcac
    27841 cgccgcggcg ccgtcagaca tctgggagct gttgccggcc gtcaccgaac cgcgggccgc
    27901 gaagaccgga cgcagcttgc cgagccccac cacggatgca tcgggacgcg gaccttcgtc
    27961 ggtatcgcag acgcgctcac ttacgcgcac cacgccgccc gcgccgggca cgtgggtgcg
    28021 cacggtgtag ggggtgatct cgtcacggaa ggcgccggcc gcgatggccg ccgcggcccg
    28081 gtgattggac tggacggcga agctgtcctg ggcttcgcgg ctgatctccc actgctgcgc
    28141 caccttctcg gcggtgaggc ccatgccgta ggcgatggcc acgttttcat ccttggcgaa
    28201 gacggccggg ttgagggaga tcttgttgcc catcatctgg ggcatgacgg tcatgctctc
    28261 ggtgccggcg gcgatcatca cgtccgcctc gcccaggcgg atgcgcgcgg cggcgtccgc
    28321 cacggcctgc aggccggagg agcagaagcg gttgatggtg atgcccggca cggtgtcggg
    28381 cagaccggcg agcagcacgc cgatgcgggc gacgttcatg ccctgctccg cttccggcat
    28441 ggcgcagccg acgatcacgt cgccgatctc gcgggcgtcc aggccgggca ccttgccgac
    28501 cacggcggac aggacgtggg ccagcatgtc gtccggccgg acattgcgga acatgccgtt
    28561 gcgcttggcc accggggtac gggtggcagc gacgatgtag gcttcctgaa tctgtttgct
    28621 cattgcgtgt ctcctgggac ggggcgacgg ccggtggacg gcgcgccccg tgtcgttcgg
    28681 ggtcgggtca gttgcgcagg ggcttgccgg tttccagcat gtgtacgatg cgctgctggg
    28741 tcttttcgga cttgagcaga tccaggaaga gcgcgcgctc cacgtcgagg atccactgtt
    28801 ccgacacctt ggcgttggtc tccacctcgc cgccgcacag ggcggtggcg gcggcgcggg
    28861 ccacccggta atcccattcg gaaatgaagc cgcccgcctg catgttgatc agcatcatct
    28921 cgcaggtggc gatgccgttg cggccggcca cggtcacgtc cttgcgcacc agcggcggct
    28981 ggtagcccgc ctccgccagg gcccgcgccc ggtgcagggc cacgtggagc agttcgcgcg
    29041 ggttgaacac gatgtcgtcg gagggcttcg cgaagcccag ttcgacggcc tgcacggcgc
    29101 tcttggagac ggtggccatg gcgacggtgt tgaacatgtt ctggatgaag gggtatacgt
    29161 cgccccccgc cgccctggat gccagctccg ccgcacgcag cgcaaattcc ttgcagccgc
    29221 caccggcggg gatcaggccg acaccggcct ccaccaggcc cacgtagctc tccagggcca
    29281 ggacacggtg gctggcgtgc atcaggaatt cgcaaccgcc gcccagggcc atgccctgca
    29341 ccgccgcgac gacgggcacc agggcgtgct tgaggcgcat ggatgcctgc tggaacttgg
    29401 cgacggtgcg ctccagcacg tcgaactgcc cgcccttgca ggcttccgcc acctgttgca
    29461 ggttggcgcc gacggcgaag ggcgcctcgt gccagatcac cacgccatcc aggtcacgct
    29521 cggcgcgggc gacagcttcc agcacaccgt ccagcacctc gtcaccgatg gcgtgcatct
    29581 tggacttgaa ggacaggatg ccgatacgtg catccttggc cggcaggtgc cacaggcgta
    29641 cgccctcgtt ctcccacagg gtgacgcccg catcggccgg cgcttcgcca aggacctgct
    29701 ccggatagag ctggcggcca tagacggcca gcgtggagcg ggccttgtcg gtctgatccg
    29761 cagcgctcca cgagccggcc gggccatgta cgcccgtacg accgtcgaac acccaggcgg
    29821 gcagcggcgc ggaggccatg gtcgcgccgg cggcgatgtc agccttcacc gcctcggcga
    29881 tgctctgcca gccggcggcc tgccaggtct cgaaggggcc ggtggtccag ccaaagcccc
    29941 agcgcatggc gaaatccacg tcgcgggcgt tgtcggcgat gtcgcccaga tggacggcca
    30001 cgtaatggaa gacgtcgcgg aagatcgccc acaggaactt ggcctgcacg tgctcgctgg
    30061 cgcgcagctg ggcgaagcgc tcggccacgt tgcggttctt gaggatggcc gccacctcgg
    30121 gcgccaccgt gccggcgctg ggccggtaat cctgagcggc cagatccagc acgtggatgg
    30181 ccttgccgac cttgcggaag atgccgccgc cggtcttctg gccgagggcg cccttggcga
    30241 tcagcgcggc aagccagtcg gggttgcggt aatacgggaa ccagggatcg tccggcaggg
    30301 tggcctgcat ggtgccgacc acgtgggcca gggtgtcgag cccgaccaca tcggcagtgc
    30361 ggtaggtggc gctcttgggg cggccgatga gcggaccggt cagggcgtcc acctcatcga
    30421 agcccaggcc gaagcgcgcg gtgtggtgca tgaccgccag gatggagaac acgccgaccc
    30481 ggttggcgac gaagttgggg gtgtccttgg cgcggacgat gctcttgccc aggcgcgtgg
    30541 tgagccaggc ttccagcgca tccagggtgt cggcgtgggt gctgcgcgtg gcgatcagct
    30601 ccaccagcgc catataacgc ggcgggttga agaagtggat gccgcagaag cggctgcggg
    30661 cggcctccgg gaagccttcc gccagggtgt tgatggacag gccggaggtg ttggtggcga
    30721 agatcgcctg gtcgcccaca tagggcgcca ccttggcgta gaggtccagc ttccattcca
    30781 tcttctcggc gatggcctcg atgatgaggt cgcagtcgcg gagcttctcc agatccgcac
    30841 cgtagttgcc cagctcgatg gcggcggccc ggtcgcgggt cgccaggggc gcgggctcca
    30901 gcttcttgag actatcgacg gccttcttgg cgatcccgtt ggggtcgcct tccttggccg
    30961 gcaggtcaaa gagaatgacc ggcacattgg cgttggcgca atgggcggcg atctgcgcgc
    31021 ccatcacgcc ggcgcccagc accgccacct tgcgaatgat cagtctgctc acgtgttcct
    31081 ccttttaatc gtgtcctgga tggacattca tcgacgcggg cggatcaccg tccgcccgtc
    31141 ttccgtgctg ttccgcgaag gctgtcgagt cacggccttc acaatttgaa caactgttca
    31201 aatcttagcg caatcatgcg cttaggtgca acatccaaaa attcagggga tttcggcgat
    31261 ggacggcaaa gcccctggcg gcgccaggaa accggccacc acaaagggtc gcaggcgcgc
    31321 cagaatggct tcgggctgtc cctgatccgc cagcgcgtag gtctcggtca tccgcatcac
    31381 atcctttccc gccatcgcat aggcgacgat gccgaccaga aagtacatgc gccacaccac
    31441 gtcctcctgg gacagctggg gcaaggcccg catgaaggcc tgacggtaac ggtccactgc
    31501 ctcccggtat tcgtccggga agaaggcctc cgtgcccgga ccgggttcgt agaaggcccg
    31561 cccgatgagc tgcttgaaca cctcgccggg gatggagtcc ttgcgggtga ggcgcagagc
    31621 cgacgacagg aaggcgtcca ccagcacgtt caccgcaatc ggccgcccat ctgcctcggc
    31681 ctccagacgg tcgagataac ttacccgact gccccggtta cccagcgcgc gggcatagac
    31741 cgcctccatc aggccctgct tggacccgaa gtgataattc accagcgcga ccggcacgcc
    31801 ggcggcgccg gtgatcatgc gcaccgacgt ggcggcaaag ccgcgctcca tgaaaagacg
    31861 ctcggccgca tcgaggatgc gggtcttggc atcgggctga ggatgttcga ggcgacccat
    31921 ggtgtggcgc attggcgagg catgcccgcg gcaacacggc ccgcgggcgc ccggccgaag
    31981 tgggatcaga aggccagcga gtactgggcg ccggcgatga aggcgttgga gttgtagtgc
    32041 ccgtccacca ccccgcgcag agcgctgctc tgatcgttat ggatgggcga ggtgttcacc
    32101 cgcagatagg caaagccgaa gtccaccagc gagcccgccg gggtcttcca ctgtccgccc
    32161 acggagtacc aggtgcggtt gctgtccggc agcgacacca ggcggtgcgg atcttccacc
    32221 ggcgactgat cgtaggccac gccaaacttg gccttccacg cgtcgttcac cttgtaggtg
    32281 gcgcccaggg ccatgcgcca ggtgtcgcgg aactcagtgt cgaggcgctg ggcggtggtg
    32341 ccgctgttgc tgttgatgat gtccacgtgg gggatgctgc tccagccggt ccacgagatg
    32401 tcgcccatca tggtccagcg cggatcgagc tgctgcacca cgctcagcac gaacacgtcc
    32461 ggcagctcga ccgtggcatt ggccgggccg ctgcgggcag cgttgagggg cgcgtaggga
    32521 ccctgcacgg tcagatcgcc cttgacggtg taacgcatct tcgagcgata ggacacgccc
    32581 accttggtcg tctcggacag ggtgaacagg gcaccggcgt tccagcccca ggtggaatca
    32641 ttggcgctga aggtgccggt ggacgtattc agcgggaaag gcaccgtgac accgaggggc
    32701 gcgctgttcg tgccgagcac gcgggtgtag tccacgtcga tgcgctgcca gttgatgccg
    32761 gcgccgatgg agaccttctc attcacgcgg taggccacgg acgggttgat gttcaccgtc
    32821 ttgatctcga aacgcaccga ctgggcgccg ccgatccagg gattgtcata ttcggtcttg
    32881 aggccgaagg gcgcaccgat gcccaggccc acgtacagat ccttgttcag cgcccaggag
    32941 gcatagaggt tgggcaggaa gccggtggag cccgcgtcgc cgccgttgcc ggtgctggtc
    33001 agggcaccgg cactggagtt ctcgttggcg aagcggaagc tcggccgcac cacggcaatg
    33061 ccggcggaga attcccggtc cttcagctgt gtcatgcccg ccgggttgaa gtagatggtg
    33121 ctggcgttct cggccaccgc cgctgaaccg gcgtaggcgt tgccgatccc gctggcgttc
    33181 tgttcgagca gctgaaagcc ggcggcagac gcctctccgg tcgcggccgc cacggccagg
    33241 atgggcatca tgcgggcaag ggtcttcatt ttcatggttg tgtctcctcg tgtggatacg
    33301 ctttagtttt ggcagcaggc cggcactgca gaacgcacgt cccggctatt ctagaaccgc
    33361 tgcatgccgc ggtcctgaaa cagattcgtg tcacgttgcg ttcatgcggg ttcctctggc
    33421 gggatttccc gcttcacccc gctcgacgcc acagccacgt aggtcagggt ggcctcggtc
    33481 accttgacca ccaccggatt ttccggattg cgctcggcga agacctccac atccaccgtg
    33541 atggacgtgc gccccacctt gacgatggtg gcgtagaagc tcaccagatc accgaccgag
    33601 accggctgtt tgaacaggaa ggcgttgacc gaaatggtcg ccacgcgacc gcgggcacgc
    33661 cgcttggcgg ggatcgcccc ggcgatgtcc acctgggcca tcacccatcc gccgaagacg
    33721 tctccggccg ggttgaggtc cgccggcatg ggcatgaccc gcaggacggg aatcttgcct
    33781 tcgggcagac gggaatcgga atcggacatg gctagctcca gacgatgttc aggggcgggc
    33841 gcgggacgcc agatcgtgca gcggaccgcc ggtgaagggc gcgaaaccgg ttccgaagat
    33901 cacgcccgca tcggccagat cggcgtcggc caccacgccc ttgtccacgc aggcctgcac
    33961 ggcttgacgt aacggcaaca cgatacgctc cgcgagcccg tccgggaggg ggcccggcgc
    34021 agatttttgc gcctttccgt tctgccacag gtaaaaaccc tgtccgctct tgcgtcccag
    34081 ctgcccggcc gccaccttgt cggccagcgt ccggggcagc accgcgtccg cgccagccag
    34141 ggccgtgccc gccgccacgg cgatgtccag ccccaccgta tccaccagct cgatgggccc
    34201 catgggcatg ccgaaggcgg tcagtgccgc gtccacggtg gccggtgcga tgccctcgtc
    34261 cacgcagcgc agcgcctcgt acatgtatgg accgagcacc gcattgacca gaaaacccgg
    34321 ctcgtcacgc accggcaggg gcagcttgtc gagggcgcgc acgaaggccg ccgcgcagcg
    34381 caagggcgcg tcggccgtgt gcccggtgcg caccacttcc accaggggca tcatcgccac
    34441 cgggttgaaa aagtgaatgc ccaccagccg cgcgggctcc cgcagcgccg tggcgatgtc
    34501 gccgatgcgc aggctggacg tattggacgc gagcacggcg tcgggccgcg cccggcgttc
    34561 gagatccgcc agcagggtgc gcttggcctc caggttctcg aagatcgcct cgatgatcac
    34621 gtccgcccgc gccacgccgt ggcccgccgg atcggggatg agccggtcga gggcgaagcg
    34681 ggcctcccgc cgcttgcccc ggaacttgcg ctcaaagcgc ttcgcggcgc gggcgatggc
    34741 cggcgcgatg cgctccgcgg actggtcctg gagggtgacg accatgccgc gcagggcgca
    34801 ttcggccgcg atgtccccgc ccatcacgcc cgcgcccacc acatgcaccc gctgcggacg
    34861 aaaatcggtg tccttgccga agcccttcag gcgctcctgc aggaagaaca cgcggatcag
    34921 gttcttcgcg gtgggcgaac ggaagatcgc ctccagcgac gacggcgaag ccgccggcgc
    34981 cgccagggca tttccgccat ggttcgccca caggtcgatg atcgcgtagg gcgccgggta
    35041 gtgctcgggg cgcgccttgg ccgccaccgt cgtacgcgcc cgggcggcca cgacgccctt
    35101 cagcggcccg ttcagcagcc tttgcccgaa gggcaggcgg cgtggcgaac gcccggacag
    35161 caggacgacg cgcgccgcgc tgtccatcac ccgcggcggc acgcagtcgt ccgccagtcc
    35221 caggcgtttc gccttgagcg catccacact cttgccggtg agcatcagat ccagcgccgc
    35281 cgccgggccg atcaggcgcg gcagacgcag catgccgccc caggccggat agatgcccag
    35341 catcacctcg ggcagggaca gccgcgtgcc aggttcgtct acggtcagac ggtagcggca
    35401 ggcgagcgcc agctccagac cgccgcccat gcagtggccg cggatcagcg cgagggtcgg
    35461 gtaggtcacc gcggccagcc ggttgaacag agcccagccg cggttcacca gcgtccgcgc
    35521 tgcctcggca ctgtcgatgc gggtgaattc ctcaatgttc gccccggcaa tgaagcccgc
    35581 gggcttgccc gagcggatcg ccaggcctgt cggcggctgg cgatccagat catccagcag
    35641 cgcaccgagt tcgtcgagaa cggcgcggcc cagggtgttg gtggcggcgt ccgccacgtt
    35701 cagggtcgcc caggccaggc cgtcggcgtc ccgttccagg gtccagtgtt tcagatccat
    35761 cacagtgcct ccaccagcat cgccccgccc tgcccgccgc cgatgcagat ggcggccacg
    35821 ccgcgcctgc cgcccgtgcg acgcaggctg tggagcagat gcagcacgat gcgcgcaccg
    35881 ctcgccccga ccgggtgccc gagcgccacg gcgccgccat ccacgttgag gcgcgccaca
    35941 tccagcgcgc cgagggcgcc gtccaggccc agttgctcgc ggcaatagcc cgtgtcctgc
    36001 caggcgcgcc ggcaggcgat cacctgggca gcgaaggctt cgttgatttc ccaaagatcc
    36061 acgtcgtgca gccccagccc gtggcgctgc agaatgggcg tggcggcatg gaccgggccg
    36121 agacccatct gctccggcgc aaggccggcc cactggctgt ccgcgatacg ccccaggggc
    36181 gtcagtccgt ggcgcaccac tgccgcttcc gatgccagca gcagccaggt ggcaccgtcg
    36241 gtgatctgac tgctgttgcc cgccgtaacg cgaccgtagt gcttgtcgaa gaagggcttg
    36301 agcttggcga gacccgaggt ggacgcatcc cggcgcaggc catcgtcggc cgtgtaaagc
    36361 gtgccgtccc ggtcgatgag gggcacgatc tcggcaaaat ggccggcatc ctgggcggcg
    36421 gcgacgcggc ggtggctctc caccgcgaag gcgtccatgt cgtcccgcgt gatgccgaag
    36481 cgccaggcca ggttctcggc ggtctgcccc atgagctggc ccacgaccgg atcggtcagg
    36541 cccttcatga tgccgatcac gggcgccaga tggccaaggc ggaagtggcg catgtgcgcc
    36601 agcttctggc cgaacttctt cgccgcgtac cagccggaca gccagcgcac catggcgtcg
    36661 gaaaacagca gcggcgcccg cgacagggca tccacgccgc ccgccagcac gagctgcgag
    36721 cgcccggtct ggaggttggc gatggccgaa tccagcgcct gcatacccga ggcgcagttg
    36781 cgcatcaccg tccagccggg caccttcacg ccgcagccca tgcgcagggc caccactcgg
    36841 ccgatattca cctcgtccgg cgaagggctg gcgcagccga ggatcacctc gtcgagctgg
    36901 tccggcgcga agggctggcg catcagcagg gcggagccgg ccgccgtggc cagatccgac
    36961 gctgcaaacg gccccggcgc gttacgggcc ttgaggaagg gcgtgcgggc cccgtcgatg
    37021 atgtagacag gtgtcgtcat gaacggcatc cgggctcagg ccgcctgacg cgcgggcaca
    37081 tccaccgtgg ccagggcgtc gcgcagaccg aagtcgtagg gatggtcgtc cacacggatc
    37141 accttgtcgc gcaggacgcc gcggcgcttg atcagggcgt gctcggccgc ggtgatgacg
    37201 cctgcggcca gggcctgatc gtagacggcg tcgacacccc cacccaccga cacggtcggc
    37261 ctgaactggc catcccgctg ggcgcggcgg acacgggcct ccacgggctc ggcgtcgacg
    37321 gtggccagca gcgcggcctc cagggcgccg acgggctcct ccacgtcggt aggcagatag
    37381 acatcggacg tgagccggtt gcgggtcggc gatggctcga tgaggagccg cgccacctcg
    37441 tgcccgagcc ggtcggaagg caccacgtag ggccgcccga ggggaaagac caccagccgg
    37501 cgcagcagca tcgcgaagaa gcggttggga aagttggcaa tggcgccttc aaaggcattc
    37561 tgggccttga acatgcagtc ccagatcgcc cagtgcatca gcggcgcgtc cgcggtgggc
    37621 cggccgtcgg cctcgtaacg cttgagggtg gcagacgcca gatacatgag cgagaggata
    37681 tcgccgaggc gcgccgacag cttctccttg cgtttcagcg cgccgcccag ggtgcccatg
    37741 gagatgtccg acaggaaggc gaaggcggcg gagaagcggc tcagctgctg gtaatagcgg
    37801 cgggtctccg gcgccacgcc gtcaggcacg gagacgaaat gggagccggt caggccggtc
    37861 accagggccc gcgccgcatt gctgagcgtg tacccgatat ggcgccagaa ggcgtggtcg
    37921 aaggcggcga gatcgccttc ccgcgcggca ttcatctcct tgagcacgta gggatggcag
    37981 cgaatcgcgc cctggccgaa gaggatcagg ctgcgcgtca ggatgttcgc gccctccacc
    38041 gtgatgccca ccgggatctg ctggtaggcg cgcccaagaa agttctgcgg acccaggcag
    38101 atgcccttgc cgccgatcac gtccatgccg tcattgacgg tctgacgcgc ccgctcggtg
    38161 acgtggtact tgacgatggc ggacaccacc gagggcttct cgcccaggtc gatggcgccg
    38221 gccgtcatca cccgcgcggc gtcgcacagg taggtgttgg cgccgatacg ggtgagcgcc
    38281 tcctcgacgc cctcgaagcg gccgatggcg gtcttgaact ggtaacgcac gcgggcgtag
    38341 gcccccacgg cgcgggccgt catcttctgc atgccggtgt tggagccggg cagcgagatc
    38401 gagcgcccgg ccgccaggca ctccatcagc atgcgccagc cctgcccggc catcgtcggc
    38461 ccgccgatga tgaagtcgag gggcatgaac acgtcctggc cctggatcgg cccgttcatc
    38521 cacaccgcgt tgaggggcag atgacggcgg ccgatcttca cgccggggtg gtcgtggggc
    38581 accagcgcgc aggtgatgcc cagatcctcc acgtcgccca gcagacggtc cgggtcgaag
    38641 agccggaagg ccagaccgaa caccgtgcag acgggcgcca gggtgatgta gcgcttgtcg
    38701 aagctcaccc gcatgcccag cacctcgcga ccctgccagg tgccgcggca gacgacgccg
    38761 gcatccggta tcgacgccgc gtccgacccc gcccacgggc tggtcagggc gaaggcggga
    38821 atctcctgcc ctctggccag gcgcggcagg taatgggctt tctgctccgg cgtgccgtaa
    38881 tgcagcagca gctctgccgg ccccagggaa ttgggcacca tcaccgtgac cgccggtgcc
    38941 gaagagcgcg tggacaactt ggtcaccacc tgggaatggg caaaggcgga aaagcccttg
    39001 ccgccgtatt cccgcgggat gatcatgccc aggaagccct tgtccttgat gtactgccac
    39061 acctcggcag gcatgtcctg gcgctgggtg cagtcccagt cctgcgtcag acggcacagc
    39121 tcggcagtct cgttgtccag gaagcgctgt tcctcatccg tgagccgcgg caccggatac
    39181 gccagcagcg tgcgccagtc cggccgtccg gcgaacaatt caccttccca ccagaccgtc
    39241 cccgcctcca gcgcgtcccg ttccgtctgg gacatggtcg gcagcgcggc gcggaaggcg
    39301 cgaaagatcg gcgccgtcac cagaccccgg cgcagcgccg gcaccaggaa cagcgcgccc
    39361 gccaccgcca ccacggcacc aacacccagc gcggcgcccg gcggcaaacc gccgaatgcc
    39421 gtccaggccg ccagccaggc cagcatggcc cccacccagg caaaaaatgg cgctcgcccg
    39481 taggcgagcg cgaccaccaa gggagggatg aaaaccagga accaccacat tgctgtctcc
    39541 tttttcccgg cgcttgtgcg cctgtcgtgt cgaatgtcgt aactgacccg gctcaggcgt
    39601 ccggttgcgg gccgaagtcc gccagcggcg cccgcaggcc acccagcagg aagctcatca
    39661 gcctcggcgc aaggcgcgac gggttctcgt cgtcgaattt tccggtcacc agttgcaagg
    39721 catcggtgcc ggcgatggcg taggacatgg cgcccatcat gaaatggaag cgccacagga
    39781 tctcgtggcg gtccacctcc ggcagcgccc ggtacagggc ggccaggaag cgctccacac
    39841 actccgcgta ttcctcggcg aggaacttgc ggacgaactc gttcggctcg gtgtaggtcc
    39901 gcccaagcag gcgcatgaag gtgtgcccgc cgccgtccac atcggccgcc atgcgcaggg
    39961 ccgtgccaaa aaaggcatcc acgatgcggc tgggctttac cggagcgtcg ccggcctggc
    40021 gctccaacgc gtccagcgcc tccaggcggg ccttgttgag atcggtcagg cgcctgcgga
    40081 agacgtcctg gatcagcaac tccttcgagc caaagtgata attgacggcg gccagattga
    40141 ccgcagcctg aacagtgatc atgcgcatgg aggtgccatc gaaaccgtgc tccatgaaca
    40201 gcacctcggc ggcatcgagg atgcgctcgc gggtgtcgga accatttttg ctgacgttgt
    40261 ctttcatggc agtcggagaa tcagggtcgc gttggtcacg tggcgcagaa gatcactcgg
    40321 cctaaagatt caaaccaaag attaaaacat ttgtttgaat catacgttta ctttgatcaa
    40381 agtcaagcac gcacggaccg gacctccatt cccgcccgga agccgtccat gacgcagccc
    40441 cgccccccga accacgacag cagccccttc acgacatttc ccatcgactc gacgaatgcc
    40501 ggatccgcct gcaagcgcac ggcacgcgtc ggaaaatgct gctcaccctg gcgacgtccg
    40561 tgggatcccc agatgccccg gacgccagca cgaccccgag ggccagggca ttcgcccaca
    40621 tgcccctcgg acaactcatc cacgcgcgca cccgcgaaca ccgcgtgcca cacgccacca
    40681 ctgcggacgg cacctcaccg cgcgccggtg gtccgggcaa caccggcctt cccggattcc
    40741 ggatcacctc tcaggccctc atccgcgaat ccagtcacag ccagatcgtg gatggccccg
    40801 aggaccaggt gaatctgcac aatcggcgcg tgcatcaatc catcgtggac gccgacctga
    40861 gcacggacga tggccgtctc gccgcccgcg cccggcgtgc gcaccgtgcg gagcgccccg
    40921 atgatcaccg tgccacctgg tccgccctga cgtaggggtt ccggaccgcg cttgacgccg
    40981 cacggaccca cgtccagtcc gacacctttc gcgaccgcgt cctcaaccgc attcaccccg
    41041 acggcacctt caggcagccc gacacaggca tcgtgcacgc cctgcatgag cttccggaca
    41101 cgctcgcgcg ggacggttgc ttccccacgg tccgacgccg gcggatggtt cacgcgcgcc
    41161 ccggaccaga cgccggagcg gtatggctgc cgcagcgggc cccaggtcct gcacacatcg
    41221 aatcgcttca annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacggtcggc
    41341 gagccctgtg gttccggccc cgaccgggct gaaaacacgg ttcgagcggt ggccattcgg
    41401 gctcccgcgc ctgcaggcgg ggcgacatcg gtgcaccacc ccaaacgcaa tgcattattt
    41461 tggtgctttc atcgcgccga agcaccataa cagtgctttt tttgcaaatc gtcggaataa
    41521 aagacctgac gattcatcta cttgcaccag catagctctg gcgtggcact tgcttggtgc
    41581 ctccatcgat tttcggagcc caaaatggag cagatcacca ccaccagcaa cgtagtcttc
    41641 gtcctgctag gcgccatcat ggtgcttgcc atgcacgccg gcttcgcctt tctcgaactt
    41701 ggcaccgtcc gcaagaagaa tcaggtcaac gccctggtga agatcctcac cgacttcggc
    41761 gtctccgccc ttgcctactt tttcatcggc tacaccctgg cctacggcgt caacttcttc
    41821 agcagcgccg aagttctggc ccagaagagt ggctatgaga tggtcaagtt cttcttcctg
    41881 ctgaccttcg ccgcggccat tccggcgatc atctccggcg gcatcgccga acgcgcccgt
    41941 ttccatcccc agagcgtcgc caccttcctg ctggtgggct tcgtttatcc cttcttcgaa
    42001 ggcatcgcct ggaacgacaa cctcggtctc cagccctggc tggaagccgc tttcggcgcc
    42061 aagttccatg acttcgccgg atcggtggtg gtgcacgcag tgggcggctg gatcgcgctg
    42121 ccggcggtac tcctccttgg agcccgccat ggccgctata ccaaggacgg caacatctcg
    42181 gcgcacccgc cctccagcat cccttttctc gcgctcgggg cgtggatcct gaccgtgggc
    42241 tggttcggct tcaacgtgat gtcggcgcag accatggaca aggtcagcgg cctggtcgcc
    42301 ctcaactccc tgatggccat ggtcggcggc acgatggccg ccatggtgct tggcaagaat
    42361 gaccccggct tcatccacaa cggccccctg gccggactcg tcgccgtgtg cgccggctcg
    42421 gacctgatgc atccggtcgg tgcgctcgtc accggcgcgg tggcgggcgt cctgttcgtg
    42481 cagctcttca ccatcaccca gaaccgctgg aagatcgacg acgtgctcgg cgtctggccg
    42541 ctgcatggcc tgtgcggcgc ctggggaggc gttgcggccg gcatcttcgg cagccaggcc
    42601 ctggggggca tcggtggcgt gagcctggcg gcgcagatca tcggcaccct cgccggcatc
    42661 gtcgtcgccc ttgcgggtgg caccgtcgtc tatggcggcc tcaaggccct gatcggcatc
    42721 cgactcgatc aggaagaaga atgggaaggc gcggacctga ccatccacaa gattactgcg
    42781 acgcccgacc gggaaaccca ctggtaaccc acgcaccccg gttcgggtcg gtcggcaccc
    42841 gaccagactc cgccttccag gcccggcacc cgccgggcct tttcatttcc gcgcaccgaa
    42901 cgcccccgcc gcacgcccac ccgacgatat ctgagtgacg gcgcacgttg cgcccacccg
    42961 gcgtggccag gcaaatacgc tcgtcatgat gcccacggtc gccgcggccg cgctgcggga
    43021 ggggccgggt gccggatgag gcgcagatca ttttttccac gcagccttaa agctcatcgt
    43081 cattcggtcg ataagtacga ggtctgtcgc cgagtgcctg caagggattc tgctccaggt
    43141 cctgtcgccc cgtcccgccg gcggggtaag acgccgggag cccgcccccg aatgacgccg
    43201 gcctcctccg cacgccggag accgccaaac taaaacgctt caattcacgg ggagaggaac
    43261 gtccaccatg catcgcacga acttgcgctc gaccacgcgc ccactcacgc tttctgccct
    43321 cgccatcagc ctcctgctcc tctccggctg cgcctcgatg aagcccgaac ccctgcagcc
    43381 cgccgagatc gtgagcacgg tggatgccga ccgggcccga ttgatgcagg gcgtggagcc
    43441 actcaccggc gtcctcagcc ttgaagaggc catcgcccgc gccatcaagc acaacgccga
    43501 acgacgctac cgcatcatgg aagaggctct ggcccagggc accttcgacg ccagcaccta
    43561 cgatctgctg cccagactcg tcgcctcggc cggctaccgc gaccgcaaca acgatctgat
    43621 ctcccacagc acggattcag tgaccggtgc cccgtccctg gccaatccct tcatctccac
    43681 cggccgcacg gcgaccacca cggatctgac cctgtcatgg agcctgctgg acttcgggca
    43741 gagctattac gccgccaagc agaacgccga tcgcctgctg atcgcccagg aacgccgccg
    43801 caaggccctg cagctcctgg tccaggacgt gcgcactgcc tactggcgtc tggtcgcggc
    43861 ccaatccctc catgaggccg tcggacaagg catccgcgag gcggaggcgg cgctggggga
    43921 tgcccgcaag gcggagaagg aaggcctgcg caacccgatg gaacccctgc gctaccagcg
    43981 caccgtgctt gagaacatcc gcatgctgga gacggtccag caggaactgt ccacggcccg
    44041 caccgaactg gccgccctcg tcaatctgcc catgtcgacg gagatcacgg tggcgccccc
    44101 cagcgcggtc ctgcagaagg cctggctcga cctccccgtc gcccgcctcg aagagcaggc
    44161 gctgatgcgc aacgccgacc tcagggaagg catcctgaac agccggatcg cccgccagga
    44221 gacccgccgg accctgctca agctgtttcc cggcctgtcc ttcaactacg gtaccaagca
    44281 cagcaccgac agctacctga tcaaccagac ctggaacgaa gccggtgtcc agctctcctt
    44341 caatctcttc aacgtcttca gcgcccccgc ccagatgcgc ctgagcgaag ccggcgtggc
    44401 cctggccgac cagaagcgcc tggtcaccca gatggcggtg ctcacccagt tgcacgtcgc
    44461 ccgcctgcag tacgccaacg ccatccatca gttcgagcgg gccgatgaga ttgccgacat
    44521 cgaggcgcgc atggccgggc acgccaggaa ccagagcgac gcggaaaagc agagccggct
    44581 ggatcgcgtt tcccagagca cgtcctacgt cctcgcccag ttgcggcgct accaggccct
    44641 gtccaacgcc cagtccgccg cgtccaagct gcaggccacc ctgggcctgg agccggtggt
    44701 cgacgccggc gcggagcgtc ccctgcccga actcaccgat tccgtgcgca aggcgctgga
    44761 cgcctggcag caggcggccc tgccggacgc cggcgggagc gcgaccggcc gcgacgctcc
    44821 gtgaggtgat catgcgcctc cggtccatcg ccctgatcat caacgccctg ctcgtcacgc
    44881 cggcctggtc ccagaccccg ccgccacgat ccgcagcggc gcccgcgcca gttgcctcga
    44941 atcctggaag cgccctcgag aaccgcgaga tccgggccca gctggcgccc cggcgctaca
    45001 ccacgctcgc ggccgagatc ggcgccaagg tgagccacct gcccgttccc gagggcggtg
    45061 ccttcaaggc aggccagtcc ctcgtgcaat tcgactgctc cctgcagcag gcccagctcg
    45121 ccaaggcccg ggccgccctc gccggggccg acaagacctg gcaggccaac agacgcctgg
    45181 cggatctgaa ctcggtcgga aaggtcgagc tggaaaccag cgaagccgaa gtgctcaagg
    45241 cccgcgccga ggtctccgcc aatgccaccc tggtcggcaa atgcgccatc gccgcgccgt
    45301 tcgccggacg gatcgccgaa cagaaggtcc gcgaacagca gtacgtgcag ccgggtcagg
    45361 cgttgctgga gatcctcgac gacagcgccc tcgaactgga attcatcgtc ccgtcccgct
    45421 ggctggcgtg ggtccggacc ggcaccgcct ttcaggtgcg catcgacgaa accggccgca
    45481 gctacccggc caaggtccag cggatcggcg cccgcgtcga tgccgtcagc cagtccgtca
    45541 agctgaacgc cgtcatcgac ggccgtttcc ccgaactgat cgcaggcatg agcggcaagg
    45601 tccagatgac gccgccagcc aatccgtaat cgcccccacg aggtcgccat gaatcgcatc
    45661 taccgctcca tctggaacga agtgagccgc agcttcgtgg ccgttgccga aaccgttcga
    45721 agccagggca agcccgcttc gagcagtgtc gtcgcggccg cagaatgcgt tgcgccttct
    45781 gcgtctggcg acccgacgtg cagcagccgt cccggctcac ggaccctgcg cagcgcgctg
    45841 aggccgctgg ccctggagca gcgcttcatg ttcgacggcg ctgcggtcgc cacggtggac
    45901 agtcacgccc acgattccac tgccgccgaa gccgccgctg ccgccgaggc gcttgccgcc
    45961 caggcggccc aggccgcgac cccggcccgc gaatcggaca aggccagccc tgccgacggt
    46021 ccaaccctga tccgcgcggt ggacccggcg ctcaacggcg ggcggcgcga ggtggccttc
    46081 atcgacagcc aggtcgtcga ttatcaaagc ctcgtgagcg gcatcccgca ggggatcgag
    46141 atccagctgg tcgatggcac gcacaacggc cttgaacaga tcgcccaatg ggctgccagc
    46201 caccacgact acgacgcgat ccacatcctc ggacacggca gcagcggcgc cctcacgctg
    46261 ggcaccgcca ccctggacgg cgacaccttc ggcgcatacc aggacaccct gggcaccatc
    46321 ggcggcgccc tcaaggccca cggcgacatc ctgctctacg gctgcaatgt cgccgccacc
    46381 acggccggaa ccgatctgat ccagtccctt gcgcgggcca cccgcgccga cgtggccgcg
    46441 tccgacgacg ccaccggcgc cacccgtctc gggggtgact gggtgctcga atccactgtt
    46501 aaccacatcg agacccgcag tctccagttc gcccagtatg acggcctgct cgccaatcct
    46561 gacgtgtggc agtggggcgc ccccgcgacg gtcgtctcgg gcgggcaggt ccgcagcttg
    46621 cccaccgaca ccttctacat ccgtcccaac ggcaccacgt ccaccgtcct caagctgccc
    46681 tcgaaccagt atttcggcta ctacgagatc aactcgggcg gccccggcta cttcgaatac
    46741 agcaccaaca acgggtcgtc gtggacaatt ctcgcgaacc actggagcgc cttcgcgcca
    46801 cccaatgcgc tggtgcgctt ccacgccagc ggcggcagcg tgagcgaggt cgatttcggc
    46861 gccgtcacct gtctggatgc caccaacacc accaccgccg ccaactcggg cttctataac
    46921 ggtggcggct acaaggtgcg tatcgacacc gcccccaccg acatcgccag caacaattcc
    46981 agcggtctgc tggtgagcga cacgcccctc ggctccgaca tcgccaccct caccccgacg
    47041 gataccggca cctccctcgg cggctactgg gccatcgaca gccagaccgt ctccggactg
    47101 ttcgggctca gcttcaatgc ggccacgggc aacaccgccc atctggtact cgcgtccacc
    47161 tccaacctgc cgagcgaagg acaaaccgcc agcgtcacgg tgcatttcta cgatgccctg
    47221 cagaccgacg tgaccggcgc gcccattggc ggccagggcg tcaccaagac ctacaccttc
    47281 accatcaagg gtccgtccca cgatctgaat ttcgggaacg acatcgccgt caaccccctt
    47341 gccggcgaca cggtcaacca gtccgaaccg gcgatcacga ccctgagcaa cggcaatttc
    47401 gtcgtcacct gggccgacat cagcaacggc ggcatctatt cccgcgtgtt cgacagcgcc
    47461 ggaaccgccc agggcgcacc gatcaccatc gacgcgggca gttcggcaac tcaaccttcg
    47521 atcaccgcgc tggccggcgg caagttcgcc gtcgtctatg gtgacgccac gacctacgac
    47581 atccgttaca agatcatcga cggcgccacc ggcagcgcct cgtccaacgg actgatcgaa
    47641 gcctatgctg tcgacggcgg tggcaatcag accggcgggt gggacggcta cgatcccatc
    47701 ggcgtcgccg cgagcgccga cgggagccag ttcagcgtca cctggaacct tggcaactac
    47761 acgacggtca agactgccgt cctcagcgac agcggcacac cgaacggcgc gatcgtcgac
    47821 ctctccggcg taaccgcggg cttcgccccc gtcacggccg ttctcgccaa tggtgacgtg
    47881 gtgaccgcca gcatcgacgg caacgcctgg gccggcttca acctcacggt cggtggcgcc
    47941 ctcgtgagcg ggggtcccgt cggcgacaac ggcaccggcg tctccatcgc caccctcgac
    48001 ggcggtggct tcgtggtggc ctggagcaag ggggatggca gccacgtcta tgcccagacc
    48061 ttcagcaacg cgggcacgcc cacttcctcc atcctgcagg ccgacagcca gggaacggcc
    48121 atgagcgccg cccgggtctc tgccctcggc ggcggaggct tcgtcgtcgt gtggcagtcg
    48181 gacggcctgg acggcagcgg cacatccatc cagggacgcc gtttcgacgc cgccggtagc
    48241 gccgtcgacg cccaggagtt ccaggtcaac gaataccgct acggcgacca gagcactccg
    48301 gtcgtcacgc cgctggacaa cggcgccttc gccacggcct gggtcgattc ggccacctcg
    48361 ggcaagacca ccggcagcga cattgaggcc cgggtcctgt ccaccgcccc gagcatcacc
    48421 tctgccacct atgacgcagc caccggcgtg ctgaacgtca ctggcgacaa cattcccgcc
    48481 aacgcatcgg gcgccgacat cgtcgcagcg ctcttcaccc tcggcggcga aggcagcgcg
    48541 acatacacgc tgacggacac ggctgacgtg gagcgcacct ccgccaccca gttcgtcctc
    48601 accctgtcgg ccaccgaccg cagccggatc aaccagatcc tcaaccgcaa cggcaccagc
    48661 gccagcgacg gcagcagcta caacctggcg gccgccgaca actgggccgc cggagccacg
    48721 ccgtcggcca acattgccga caccaccggc aacgcgatca cggtctccaa cgtggccctg
    48781 cccgccatca ccagcgccac ctacgacgcg ggcaccggcg tgctcaccgt gaccggcagc
    48841 ggcctcaccc accacgccgg cagcgccaac gacatcgacg tcacgcgcct caccctgacc
    48901 ggcgcgggcg gagcgaccta caccctgacc gccagcacca gcgatgtgga catcaccagc
    48961 gacacatcct tcaccctcac cctgggcagc accgacaaga ctgccgtgga tagcctgctc
    49021 accgccaatg gcacccaggt gggcggcgtc acctacaatc tggccgccgc cgacaactgg
    49081 gaacgcggcg ctgacagcag tctgaacatc gcggacaccg tcaatggcgt cctggtcagc
    49141 ggcctcaacc cggcgccgac catcacaccg cccgccgaca tcaatctagc cgacaccggc
    49201 gcgacggaca ccttcgcggt ccagaccggc accctcagcg ccacggacgc agacggcatc
    49261 gcggcgttcg gcatccagtc gcccaccagc accaacgccg gcttcaccca tggcggcatc
    49321 acctacgacg tggcccgcag cggcacctac ggcaccctgt acctggtcag caccggcgcc
    49381 gacgcggggc gctatgccta tgttccggac gccggtgcga tcaatggatt gacagcaggt
    49441 gccacgccca ccgacgtctt caccgtcacg gcaaccgacg ccaacgccag cccggccacc
    49501 ggcacggcca ccctgaccgt ccacctgacg ggcgccaacg acgcgccgac cctctcggcc
    49561 accggcctgt cgcccacctt caccgagaac ggcagcgccg tcaccctgtt ctccggcgcc
    49621 agcgcgtcgg ccgccgaatc cggccagaac ctgaatcagc tgatcctcac ggtcagcaac
    49681 gtggcggacg gcagcagcga gcttctccag gtcgatggca gcacgctggc cctcacccac
    49741 ggcaacagcc tcaccaccgc gtcccatggc gtctcggcca gcgtcagcct cagcgtgggc
    49801 gtcgccaccg tcacgctgga caaggcgggc ggcctgtcca cctccgtcat gcagtccctg
    49861 atcgacggcc tgggctatcg caacaccagc gaatcgccca gcacctcgaa ccgcgtggtc
    49921 accctgacga gcctgcgcga cacgggcggc acggccaatg gcgggcagga caccaccgtg
    49981 ctcgccattg cctccacggt caccgtcgtc agcgtcaacg atgcgcccac ggacatcacc
    50041 ctcagtgcca gcagcctgaa cctttccgcg gcaaccgcca gcgcgccggt cgccactctg
    50101 ggcgtggcag acctggacac gagcgacacc cacagctatg ccctcgtcag cggcagtggc
    50161 gatgccgaca acggccggtt cgtcctcagc ggcaacacct tgcaggtggg gggcgcggcg
    50221 ctgagtgccg gcacctattc cctgcgcctc caggtgaccg acaacggcgg tctcagctac
    50281 accaaggcgg tcacggtcac ggtcgtggac gatgtggcgc cgaccctcga cgcgcccggc
    50341 tccacccccg ccggcgacgc aagcggcgtt gcccccagcg ccaacctcca gctgcacttc
    50401 gacgaggcca tcgccttcgg cacgagcggc acgatcaccc tgcgcaacgt caccaccggc
    50461 acggtggtcc agacctggaa cgtggccacg ccgggcgacc tcggcagtgg taatggccag
    50521 atcgccatca gcggcagcac cctcaccctc aaccccacgg ccacgctcga tgaggccagc
    50581 cgctacgccg tccgcatcgc tccgaccgcc ctgcgcgaca ccgcgggcaa tgccttcgcc
    50641 ggcatcgccg acgactcgac ctaccgcatc acgaccggcg tcaccgactc ggcaccgccc
    50701 gcgctgctgg ctatccagcg cgcctcaccg tccaccgagc tgacccgcag cggcagtgtg
    50761 agcttcaccc tgcgcttcaa cgaagccgtc accaacgccg accttgccga cttcgccctg
    50821 agcacctcgg ggaccgctac cggcaccctc ggcagcgtca ccggcaacgg caccgacacc
    50881 ctcacggtga ccgtcaccgg cgtcagcggc accggcaccc tcggcctgag cctcggcggc
    50941 ggtcacacga tcaccgacat cgcgggcaac gccctgcccg gcggcaatcc ggccgacacc
    51001 gagctctacc acgtggaccg ggatgcgccg gggctcgtct ccatcaaccg ggtgacggac
    51061 gacgacgtgc tggcgagcgg gacagcccag ttcatcgtcg tattccggga gcccgtcacc
    51121 caggtcgacg tcggcgactt caacctgctc acctccggct ccgtcacggg ccagatcacc
    51181 gcagtggccg gcaacggcag ccccgcgatc accgtcagca tcggcaacat cagcggcaac
    51241 ggcaccctcg gcattgaact cgccagtggc gccaccgtca cggacgtggc cggcaatgca
    51301 ctgctcagcg ccacgcccct ggccggtatc gccgaacgct accaggtgga ccgtgcggcc
    51361 cccactgcgc tgggcattac ccgcgtcggg accgagcgca cccgcagcgg ctccgtcagc
    51421 ttcaacgtcg tgttcagcga acccgtcagc cacctggacg cgaccgactt caccctcgtc
    51481 acctccggca ccgccgccgg cacggtcacc ggcgtgagcg gcagcgggag cagctacacc
    51541 gtcaccgtgg gcagcctcac cggcagcggc accgtcgggc tcgcctttgc cggtccccag
    51601 gacgtcgccg acgctgccgg tctcgcgttt ggcggcacgg cgccggcgct gaacgaggcc
    51661 ttcgtcctcg acaacacgcc gcccacggtt accgccatcg atcggggcgg cgtcaatcag
    51721 atcgccgcca atacgccgtc ggacgcggtg ttcaccgtgg tcttcagcga gcccgtcagc
    51781 aacctgtcgg cggccgactt caccgtcacc ggcagcgcag gccacggcag catcagcgcc
    51841 gtggatagcg cggacgggaa ggtcttccac gtcaccgtga ccggcgtcaa tggaaccgtc
    51901 ggccagaccc tcgggctgag ctacagcggc agcgtggacg acgccgtcgg cctggcgggc
    51961 acctcgccct tcagcgccgg acaggtctac acggttggcg gcgtgctcct caacgaaggg
    52021 gccatcgacc aggcgacgct ggacgcgctc gccggcgtca atcgcgccgg caccgagcgc
    52081 ctggtgaccg catcggcccc ggtgacccag gtggtcatcc tcgacagccg ggtgcccgga
    52141 ctggccggcc agctgagcgc gctgccggcg ggcacggagg tgtggctgct cgacgccggt
    52201 accagcgcga cggcccagat cagccagatc ctggcccact acagcggcct gtcggcgatc
    52261 cacctgatct cccacggcag cagcggcgcc atttatctcg gcgccgaaac gctctcggac
    52321 gccaccctgg cacagtacgc ctccgcactg gccgcctggg gcgccgccct cagtcccagc
    52381 ggggatctgc tggtctatgg ctgcgacgtg gcccagggcg ccgtcggcca gcgcttcgtg
    52441 gccgccctcg ccgccgccac aggcgccgac gtggccgcat ccaccgacct gaccggcgcg
    52501 cccttcctgg ggggcaactg ggtgctggaa gacacggtcg gcacaatcga tactggcacg
    52561 ctccaggccg acggctacta cggcacgctg agcccggtca ctttcgattt caccccttcc
    52621 aatggtgtcg atacggggac aagcggttac gtgaaacagc tcgtcagcgg cgtcaaggtc
    52681 actggcacca tctacaccac gtcagccggc acgacgatca ccggcagcgg cttcggtccg
    52741 accgtgtcca gcaatcgctt caccgacacg accaactccg gccgcatgat gcagttcacg
    52801 ttcagcacgc ccagcgacgt gaccagtctg gtggtgggct actccgccaa ctccggcacc
    52861 atctccggct tcaccgtcaa gggttaccag tccgacagca cggtggtcac cctgaatgtg
    52921 acccccacct tcaatgtggg ctacaccgtc agcctgaccg gcgccaacga ctggtccaat
    52981 ctgaccaaac ttcaggtcat cgccaatggc ggcggcagcg tcaccttctt tgccgacacc
    53041 atcgtcgcca cgccgagcaa ccctgcgccg accgacatcc agctgtcgtc cgtgaacgcg
    53101 cccgccaacc tggccggcgc gcgcatcgcc aacatccgcg cgtccgacag caccgggctg
    53161 atcagcacgc cctccagtta ttccagcccg aacgacatct tcttttcgga caccgagact
    53221 tacaccgaag tcgccgatcc aagcgatctg ttcactgtcg attccaccgg cctgctgtct
    53281 ttcagtgccg gcaagtccct gggcaacggc gccacggcga gcatcaccct gcgcgccacc
    53341 gactcggccg gcaacacctt cgacaaggcc ttcacgatca ccggaagcac ctacaacacc
    53401 ctcacggtca gcccgtccca gctgcagaac ttcacctatg gcggcgccca gatcggcacc
    53461 aactacgcca gcacggccaa ctggaccggc tcgtggggca attccgacgc cttctacgtc
    53521 ctcgccccgc aaggcgccgg cgccgatgtt ttcgacaacg actggagcga ctcgctcgtc
    53581 aacaccatcg actacggcgc cctgagcacc aatggcgcct acgacagcct cgccttccag
    53641 gccggcgcca gcgccggcag cgacagcttc gccatcggca gccagtacta cgtggtgacc
    53701 gtcggcacgc ccaatgcgcc gcccgtcgtc gacctgaacg gcaccaccgg aagcggcgca
    53761 tcggccgcgt tcgtcgaagc cacccaccag ggcacgggca gcaccagcgg ggtcgtcctc
    53821 gtccccgcgg cgctactgtc cgacagcgac ggcaccgtca atacggtcgt gctgtccctt
    53881 gcgaacagcc agtccgacag tggcgagcgc ctatacctct cgggcagttt cagcggcgtc
    53941 acctattcgc tggactcgtc cacccagatc accctcaccc gcaccggcac caccgcagac
    54001 ctccagtccg ccctgcgcgc ggtccgctac cagaacaatt cggacaatcc gaacgtgacg
    54061 acccgaagca tcaccgtcac ggccaccgac aacagcggcg gatcgtccgt cgagacggtc
    54121 accgtctcca tgacccgcag caatgacgca cccaccgaca tcagcctgtc gtccgccacc
    54181 gtcaaccagt ccggcgggac caacgccgtc gtgggcacct tgtccaccac ggacgtggac
    54241 tccggaagct tcagctattc cctggtcagc ggcaccggag acaccgacaa cagcctcttc
    54301 aacatcgcct caggcagcct gcgggccaac gatgccgccg cgctggcggc aggcagctac
    54361 agcgtgcggg tacagaccag cgacggctcg ggcggcacct acgacaagat cttcacgatc
    54421 acggtcgccg acaatgtcgc gccggtcttc gacgtggccc ccgcgaccgc caacgtcacc
    54481 accgacggct tcgacatcac cgccagcctg aatgaagcgg gaaccgtcct gtacgtgatc
    54541 gtcccccgta acgccgctgc gccgtccgtg gcccaggtgc tggccggtca ggacgcgtcc
    54601 ggcagcgcgg cgttgggcag cggcagtcag agcgtgccgt ccgcgcccta ctccgccacc
    54661 gtcccggtga gcggactcag cctcggcacg gactatgacg tctatgtcgt ggcccgcgac
    54721 agcgcctcgc ccgccaacgc ccaggccgcg gcggtcaagc tcagcgtctc gaccctgtcc
    54781 aacaccgccc ccgcgctggg cacccccacc gtcatcgcgc tgaccgacac cgccgccgcg
    54841 gacaccttca gccacccggg cggccagctc agcgccacgg acaccgacgg catcgcccgg
    54901 ttcggcatcc agtcccccac cagcaccagc gccggcttca ccgacaacgg catcacctac
    54961 gacatcgccc gcaccggcac ctacggcacc ctctacgtcg taagcaccgg tcccgacatg
    55021 ggccgctacg tttacgtgcc cgacgccacc gccatcaacg ccgtggccgg tggcgccacc
    55081 cccagcgaca gcttcaccgt caccgccacc gacgccaacg ccagcccggc caccggcacc
    55141 gccacgctca cggtaaatat cagcggcgcc aacgacacac ctgcgccgcc caccctcaac
    55201 ggcggcagcg cgatcaacgc caacgaatcc gccacgctcg cggccgccac cctgggcacc
    55261 ctggccgcca ccgacgcgga aaacgggacc ctcacctaca cgctggtggc cggcagcggc
    55321 gacgcggaca acgcgcgctt cagcatttcg ggcacttcgc tccgggtcaa tgccaccccg
    55381 ctcgccgcgg gcacctattc gctgcgcgtg cgagtcacgg acaacggctc gccggcacaa
    55441 tccgccgagc aggtcttcag cgtccaggtg agcgacgacg tcgcgccgac gctccagccg
    55501 gccggcagca cgccccagaa cggcgccacc ggcatcgccc cgacggccca gctggaaatg
    55561 gccttcagcg aaaacgtgca tgccggaagc ggcaccgtca cgctggtgga cgtcaccacc
    55621 ggcgccgtgg tcgagacctt cgacgccgcc accggcatcg gcagccacgg aggcacgctg
    55681 tccttctcgg gcactgcgct ggtcgtcacc ccggggagcg cgctcgatca ggcccatcaa
    55741 ttcgccctgc aggtcccggg aaccgcagtg atcgatggca gcggaaacgc ctacgccggc
    55801 acctccggca acaccgacta ccacttcacc accggagcgc tcgacaccac ggcaccccgc
    55861 gtcaccctgg tggacatcgc cgatccggtg caacccaacg ccggcaccgt gagcatccgc
    55921 ttcagcgaag cggtgcagaa cgtgggcatc agcaacttcg tgctcacccg cgacggcgta
    55981 ccggtggatc tcagcgggct cagtgtcggc ggcagcggtg cgacctacac cctcgacctg
    56041 tcctccgtca ccggcaccgc cggcacctat gtcctgacgc tcgccccggg cagcatccag
    56101 gacagctcgg gcaacgccct ggccgccggc gccagcgaca gcttcgtcgt cgacaccact
    56161 gcacctgcca tcgcggccat cacacgcgcc tcggccacgc ccacccaggg cggcagcctg
    56221 gacttcaccg tggccttcag tgaagcggtc agcggggtcg atgtggccga cttccagctg
    56281 tccggcacgg ccaccgcagg cgccggcatc gcgtccgtca cggcgctttc agacagcgtg
    56341 tatcgcgtca ccgtcaccgg cgccaacggc aatggcaccc tcggactcga cctgcgtccc
    56401 agcgccaccg gcatccagga cagcgccggc aacgccatag gcggcggcgc gacgggccag
    56461 cgctacaccc tcgacaacac agccccgggc gtcacgagca tccgccgcag tgctgcggaa
    56521 ctcagcgccg gcggggcgct gtccttcacc gtgtccttca gcgaagcggt caccggcgtg
    56581 gacgcctccg acttcaccgt agataaggcc gccggcgtca atgcccagga cgcggacatc
    56641 agtgtcagcg gcagcgggaa cacctacacc gtgaccgtcg cctccgttgg cggcaacggc
    56701 ctcttcagcg tcgacctcaa agccagcgga accggcatcg ccgaccggac cggcaacgcc
    56761 ctcgtcggcg gatacacggc cggtgacacc tacctcctgg acagcaccgc ccctatcgtg
    56821 gcgccgtccc aggccttctc gatccccgag aacatggccg ccggattcat catcggccag
    56881 gtccgggcca gcgacgccaa ccgcctcgcg tcgttccgca tcgccagcgg tgacccaaac
    56941 ggctacttca ccatcgacag cgacggcgtc gtccggctgt cggcggccgg cgccgcgccc
    57001 aacgcgccct cccgcgacta cgagaccgcg cccaacctgt tcaccctcga catcatcgcc
    57061 accgacgcgg ccgggctcga cagcgccacc cagagcatca cgatccaggt gctcgacgcc
    57121 atggaaaacg ccggcgcccc cgtcttcacg acaccaaccc ctgtcagcct caacgacacc
    57181 cgcagctacg acaccctgac ccctgccagc ggcacgctgc aggccacgga cacggacggg
    57241 atcgcccgct atggcatcga caccggcacc tccgggggga gcaccgtcat cggcggtgtc
    57301 tcctacgacg tctccaggac ggggctgtac ggcaccctct atctcaacag ctcgaccggc
    57361 gcctatgttt acgtgccgga cgccgcagcg atcaacgccc tgcgcaacgc cacgagcgaa
    57421 tccttcacac tcacggccac cgacgccaac gccacgccgc tttccagcag cgccacgctg
    57481 acgatcgaca tcaccgccac caacgacgat ccggtgctgg acgtgcccgt cccgccccag
    57541 acctttgccg ggcccggacc gtggaccttc accgtccccg gtggcagcgt cagcgatccg
    57601 gaaggcgaca gcctcacctg gagcgccacc ctcgccga
//
LOCUS       DAOQMM010000011        50892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_141_length_50892_cov_50.846844, whole genome shotgun sequence.
ACCESSION   DAOQMM010000011 DAOQMM010000000
VERSION     DAOQMM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 50892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50892
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141_length_50892_cov_50.846844"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..636
                     /locus_tag="PLH85_03255"
     CDS             55..636
                     /locus_tag="PLH85_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB63604.1"
                     /translation="MPDDTSYYVYALKDPRRSPALPFYIGKGTGTRAHDHLIKPDGTR
                     KGARIREIVDSGGHVLVTRLVDALTEQQALSLEAELIAAFGTLDTGGLLTNTVVPSGL
                     SRMTKPAIVVPSGVREKAQLGLALLKDAILEMAKANPRGITNSDAASLLGLRSDYGGG
                     SKDYLSYSVIGLLMREGKVQRDGASRRHIARVE"
     gene            complement(647..1393)
                     /gene="tatC"
                     /locus_tag="PLH85_03260"
     CDS             complement(647..1393)
                     /gene="tatC"
                     /locus_tag="PLH85_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNB63605.1"
                     /translation="MNAPQEESFISHLVELRDRLLRAVVAVVLVFVCLMPWAGDIYDL
                     LALPMMANLPEGTRMIATGVVTPFFVPVKVTLLVAFVIALPFVLYQAWAFIAPGLYAH
                     EKRLGLPLVIGSTVLFLAGMAFCYFFVFGTVFKFIAQFAPKSITPAPDIEQYLAFVMS
                     MFVAFGITFEVPVIVILLVKIGLVDVAKLKEVRPYVIVGAFIVAAVVTPPDVVSQLML
                     AIPMCLLYEAGVFLARFVVKREVGVESGSA"
     gene            complement(1390..1977)
                     /gene="tatB"
                     /locus_tag="PLH85_03265"
     CDS             complement(1390..1977)
                     /gene="tatB"
                     /locus_tag="PLH85_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNB63606.1"
                     /translation="MFDIGFSELIVIGVVALIVLGPERLPKVARTTGHLLGRLQRYVA
                     DVKSDIHREMQIDELKRLQDEARQAALQMETSVRDEMAHFKGEVDSAVQGAASSVAEL
                     ERELQAAAPSAEPDAQSEAPSAPSASPSADVTDADAAAQRLDRQLAEAEQHAGAPVFA
                     EAQPDGSDAAMPDDRQLGFDFAAAEAAPAAKAEQS"
     gene            complement(2097..2321)
                     /gene="tatA"
                     /locus_tag="PLH85_03270"
     CDS             complement(2097..2321)
                     /gene="tatA"
                     /locus_tag="PLH85_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNB63607.1"
                     /translation="MGSFSIWHWLIVLVIVMLVFGTKKLRNIGEDLGGAVKGFKQGMR
                     EGEGAAGDPQKIAGAEGRTIDAEAREKNKS"
     gene            complement(2332..2682)
                     /locus_tag="PLH85_03275"
     CDS             complement(2332..2682)
                     /locus_tag="PLH85_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNB63608.1"
                     /translation="MQECLFCRIVRGEIPAAKVYEDDEVLAFKDIHPQRPVHVLVIPK
                     HHITSLAHASEADVPVLGRMLAVAHRIAADQGSPNGFRSIINTGPVGGQEVMHVHMHI
                     VGGQEPVGPMLRRI"
     gene            complement(2695..3021)
                     /locus_tag="PLH85_03280"
     CDS             complement(2695..3021)
                     /locus_tag="PLH85_03280"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNB63609.1"
                     /translation="MIDIEVLHRVAQTLAERKEADPESSYVSGLFHKGTDAICKKVAE
                     EAAETIMAAKDKDLLHVVWEVTDVWFHTMILLAHFGLSVDDVLAEFRRREGVSGIDEK
                     KARTAK"
     gene            complement(3018..3407)
                     /gene="hisI"
                     /locus_tag="PLH85_03285"
     CDS             complement(3018..3407)
                     /gene="hisI"
                     /locus_tag="PLH85_03285"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNB63610.1"
                     /translation="MSEAWLDAVAWDAQGLVPAIAQDAETGEVLMFAWMNRDALARTV
                     ATGEAVYFSRSRGRLWHKGEESGHIQKVRELRIDCDNDVVLLKIEQIGGMACHTGRRS
                     CFFQKYFADGRWQAVDPVLKDPKDIYK"
     gene            complement(3404..4162)
                     /gene="hisF"
                     /locus_tag="PLH85_03290"
     CDS             complement(3404..4162)
                     /gene="hisF"
                     /locus_tag="PLH85_03290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNB63611.1"
                     /translation="MLAKRIIPCLDVNAGRVVKGVNFVELRDAGDPVEVARRYDEQGA
                     DEITFLDITASSDDRNIILHVVEQVAEQVFIPLTVGGGVRTVDDVRRLLNAGADKVSI
                     NTAAVNNPQVVAEASGKVGSQCIVVAIDAKQVAPGRWEVFTHGGRNRTGLDAVEWARK
                     VESLGAGEILLTSMDRDGTKNGFDLGLTRAVSDAVNIPVIASGGVGNLDHLADGVSLG
                     RADAVLAASIFHFGDHTVREAKELMRARGIEVRL"
     gene            complement(4167..4913)
                     /gene="hisA"
                     /locus_tag="PLH85_03295"
     CDS             complement(4167..4913)
                     /gene="hisA"
                     /locus_tag="PLH85_03295"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNB63612.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGEMDAATVFSEDPRAMARHWLDQGA
                     RRLHLVDLNGAFAGKPKNEAAIRSILAEVGDEIPVQLGGGIRDLDTIERYLDAGLSYV
                     IIGTAAVKNPGFLHDACSAFAGHILVGLDAKDGKVAVDGWSKLTGHDVVDLARKFEDY
                     GVEGVIYTDIGRDGMLSGVNVEATVKLAQALRIPVIASGGIAAMADVEALCAVESEGI
                     TGAITGRAIYEGTLDFSAAQARADALSGAA"
     gene            complement(4977..5618)
                     /gene="hisH"
                     /locus_tag="PLH85_03300"
     CDS             complement(4977..5618)
                     /gene="hisH"
                     /locus_tag="PLH85_03300"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNB63613.1"
                     /translation="MSSVAIIDYGMGNLRSVAKAIEHVATGATVVVTADPAAVLAAER
                     VVFPGQGAMPDCMRELDARGLRPAVLEAARSKPFLGICIGQQMLFDHSDEGDVPGLGL
                     FRGHVRRFPSDARMTAPDGGRLKVPHMGWNEVWQKQAHPLWAGIPDGSRFYYVHSYYV
                     DPADPAVIAATTEYGIPFTGAVAQANIFAVQFHPEKSAQAGLQLLSNFMSWKP"
     gene            complement(5620..6207)
                     /gene="hisB"
                     /locus_tag="PLH85_03305"
     CDS             complement(5620..6207)
                     /gene="hisB"
                     /locus_tag="PLH85_03305"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNB63614.1"
                     /translation="MRQAEISRDTLETQIRVRLDLDGSGQSRLDTGIGFFDHMLDQIA
                     RHGCFDLEIAAKGDLHIDGHHTVEDVGIALGQAFARAIGDKKGLTRYGHAYVPLDEAL
                     SRVVVDLSGRPGLEFDVPFTAAAIGALDTQLVYEFFQGFVNHAGVSLHVDNLKGRNAH
                     HQCETVFKAFGRALRMAVTPDPRQAGVVPSTKGAL"
     gene            6317..7408
                     /gene="hisC"
                     /locus_tag="PLH85_03310"
     CDS             6317..7408
                     /gene="hisC"
                     /locus_tag="PLH85_03310"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNB63615.1"
                     /translation="MSRFWSPVVHDLTPYVPGEQPKLANLVKLNTNENPYPPSPRVVD
                     AIRAELGEDGDRLKLYPDPTGSALKAAVSRQFGVPAEWVFVGNSSDEVLAHTFQGLLK
                     HDAPLLFPDITYSFYPVYCGLYGIPFETVPLAEDFSIRVDDYLVRGPAAVGGVIFPNP
                     NAPTGRLLPVAEIARLAAGLPDSPVVVDEAYVDFGGHDAIPSAVALVAEYPNILVTQT
                     LSKSRSLAGLRVGFAIGQPELVEALTRVKDSFNSYPLDRLALVGACAALDDTEWFHCT
                     RHQVIASREALSASLTNLGFEVLPSAANFVFARHPAHDAALLSAALRARSIIVRHFRA
                     PRIDQFLRITVGTDAQCAQLVTALGEILG"
     gene            complement(7490..8806)
                     /gene="hisD"
                     /locus_tag="PLH85_03315"
     CDS             complement(7490..8806)
                     /gene="hisD"
                     /locus_tag="PLH85_03315"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNB63616.1"
                     /translation="MSEPIRRLASSDAGFAATLDALLAFEGSTDAAIEAAVADILAAV
                     RRDGDAAVVEFTRRFDHVDAADMAALELPRAELAAALAGLPAEQRTALEAAAERVRRY
                     HLRQVQESWSYVEDTPDMRGTRLGQQVTPLDRVGLYVPGGRAAYPSSVLMNAIPAKVA
                     GVGELIMVVPTPRGEKNPLVLAAAAITGVDRVFTIGGAQAVAALAYGTGSIPQVDKIV
                     GPGNAYVASAKRRVFGTVGIDMVAGPSEVLVISDGSGDPDWVAMDLFAQAEHDELAQS
                     ILLCTDADFIARVAASIVRLLPDMPRRDTIATSLKNRGALIHVRDLAEACDIANRIAP
                     EHLELSLAEPGAWVERIRHAGAIFIGHHSVEALGDYCAGPNHVLPTMRSARFSSPLGV
                     YDFQKRTSLIEVSEAGAQTLGRIASVLARGEGLQAHARSAEMRLKD"
     gene            complement(8803..9447)
                     /gene="hisG"
                     /locus_tag="PLH85_03320"
     CDS             complement(8803..9447)
                     /gene="hisG"
                     /locus_tag="PLH85_03320"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNB63617.1"
                     /translation="MNGITLALSKGRIFDETLPLLAAAGIVPTDNPESSRKLIIGTNR
                     PDVRLVIVRATDTPTYVQYGAADLGIAGKDVLIEHGGVGLYQPLDLNIAKCRLCVATP
                     KDFDYAAAVKRGARIRVATKYVKTAREHFAAKGVHVDLIKLYGSMELAPLVGLADAIV
                     DLVSTGGTLRANNLVEVEDIMPISSRLVVNQASLKLKRELIQPVLEAFAGAVKP"
     gene            complement(9524..10774)
                     /gene="murA"
                     /locus_tag="PLH85_03325"
     CDS             complement(9524..10774)
                     /gene="murA"
                     /locus_tag="PLH85_03325"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNB63618.1"
                     /translation="MDKLLIEGGARLSGEIAISGAKNAALPILCAALLTKEPVTFTHV
                     PRLNDIGTLLRLLEQMGVKVSRDGDTVTLDAGGVNNPVASYEMVKTMRASILVLGPLL
                     ARFGEARVSLPGGCAIGARPVDQHIKGLQAMGADVKVEHGYVNARSPRLKGARIFTDM
                     VTVTGTENLLMAACLADGETVIENAAREPEVVDLANCLVAMGARISGAGGDVIRIQGV
                     PALHGATHRVMPDRIETGTYLCAAAVTGGEVRLTHTSTSYLDAVVDKLMDAGCEITAE
                     RDAIRLKAPARLTAVSLRTAPYPAFPTDMQAQFMALNTVADGTAVIRETIFENRFMHA
                     VELQRLGADIRIDGNTAVVKGVAGLQGATVMATDLRASASLVIAGLVAEGETTIERIY
                     HLDRGYERLEAKLAALGARVTRLK"
     gene            complement(10788..11042)
                     /locus_tag="PLH85_03330"
     CDS             complement(10788..11042)
                     /locus_tag="PLH85_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNB63619.1"
                     /translation="MFLASEIERLIGAGLDCEFLQVEGDDGTHFSGIVVSAAFEGKPK
                     VRQHQAVYATLGKLMGNEIHALQLTTYTPAQWADFQKELG"
     gene            complement(11052..11807)
                     /locus_tag="PLH85_03335"
     CDS             complement(11052..11807)
                     /locus_tag="PLH85_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63620.1"
                     /translation="MEGFKTLFYKECLRFWKVGFQTVLSPVLTAVLYLLIFGHVLEDH
                     VQPYPGVSYTAFLVPGLVMMTVLQNAFANSSSSLIQSKITGNIVFVLLPPISYREFYA
                     AYVLAAILRGALCGLGVLLVSLPFAMPGVAAPGWIVAFTLIGGAFLATLGVIAGIWAD
                     KFDQLAAFQNFLIMPLTMLSGVFYSIHSLPGFWQGVSHFNPFFFMIDGFRYGFFGVSD
                     VSPWTSVGVVSVCFIVLAVATLQMLARGYKLRA"
     gene            complement(11811..12713)
                     /locus_tag="PLH85_03340"
     CDS             complement(11811..12713)
                     /locus_tag="PLH85_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB63621.1"
                     /translation="MIPAIRAVGVTKRFRALQALAGVDLEVAQGEFFGLLGPNGAGKT
                     TLISALAGLARADAGTLEVMGHDVVRDYRAARLALGVVPQELVFDPFFSVREMLRIQS
                     GYFGLKRNDDWIDEILHHLDLTPKAHANMRMLSGGMKRRALVAQALVHRPPVIVLDEP
                     TAGVDVELRQGLWQFIRKLNRDGHTVVLTTHYLEEAETLCGRIAMLKAGRIVALDTTA
                     NLLARFANHTLQVRLAAPVSLPPALAAAVVRQDGLDLVLGFERFDDVGALLDALRGAG
                     AQIADMVLGKPDLEQVFVGVMKES"
     gene            complement(12733..13017)
                     /locus_tag="PLH85_03345"
     CDS             complement(12733..13017)
                     /locus_tag="PLH85_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB63622.1"
                     /translation="MIVQDGDCLRLSGALTLTTVSGLLEAGRAAVAAGDCVADLSAVT
                     RVDSAALALLFAWLRAARSAGHRLSLVNAPTALVSLASLYDVDGLLPPAA"
     gene            complement(13014..13643)
                     /locus_tag="PLH85_03350"
     CDS             complement(13014..13643)
                     /locus_tag="PLH85_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB63623.1"
                     /translation="MIKKTASFLAALTLSLGFQAAAFANAVAPDALVKGVTDDVIAIV
                     RQDKAIQSGDTRRAVELVETKVLPYFDFAHMTRLAVGKDWKSASADQKSALTDEFKTL
                     LVRTYSNALTQYRNQQIDFKPLKAKPEDTDVVVRTEVRQPGAKPVQIDYALDKQGDAW
                     KVYDVMVAGVSLVTNYRDEFGQQIRSGGVDGLIKSLKDKNKQLEAAPKK"
     gene            complement(13640..14452)
                     /locus_tag="PLH85_03355"
     CDS             complement(13640..14452)
                     /locus_tag="PLH85_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNB63624.1"
                     /translation="MKKNSLNFLRVPARIATTLLLAGGLGACASSGGNPQDPWESMNR
                     ATFRFNDMADRAVMRPVAEAYDTVAPLPIKTGVSNVFGNVADLWIGVNNLLQGKVLDG
                     LSDGGRFLINTTVGMLGVFDIATELGLDKHDEDFGQTLGAWGVGSGPYLVLPFLGPST
                     LRDGTSLLLVDLNMDPVWQIDDMGQRNRTTALRLVAKRAQLLGADTAAEQAALDKYGY
                     MRSFYLQYRQNQVYDGSPPRVKDPDDDAALDVPAAAVPPLAAAPSEQRENQE"
     gene            complement(14502..14978)
                     /gene="mlaD"
                     /locus_tag="PLH85_03360"
     CDS             complement(14502..14978)
                     /gene="mlaD"
                     /locus_tag="PLH85_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNB63625.1"
                     /translation="MSRTTLDLWVGVFVAMGIAALLFLALKVGSISGSDVQSPYTLSA
                     RFDNIGGLKVRAPVKSAGVLVGRVAEIRYDNERFQAEVVLSVDGRYKFPTDTFANILT
                     SGLLGEQYIGLDPGGEEKTLAPGEVIKKTQSAVVLEKLISQFMFNKAADGDQAPAK"
     gene            complement(14981..15760)
                     /gene="mlaE"
                     /locus_tag="PLH85_03365"
     CDS             complement(14981..15760)
                     /gene="mlaE"
                     /locus_tag="PLH85_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNB63626.1"
                     /translation="MLDILRRLGAAAIDALWQLGFIARFFWLILVYSGQSFLRLNLTL
                     RELYFSGVLSLLIILVSGLFVGLVLGLQGYETLQRYGSADALGTLVALSLVRELGPVV
                     AALLFASRAGSAVTAEIGLMKVTEQLKAMDMMAVSPIARVVAPRFWGGVISMPLLAAL
                     FSAMGIFGGWLIGVIFIGVDDGAFWSQMQGAVDFRYDVLNGVIKSAVFGVAVALIAVF
                     EGYDCVPTAEGVSRAITRTVVSSALAILALDFVMTSFMFRG"
     gene            complement(15760..16563)
                     /locus_tag="PLH85_03370"
     CDS             complement(15760..16563)
                     /locus_tag="PLH85_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB63627.1"
                     /translation="MSEPLVRLEHVRFAYGERAILRDISLSIPRGQVVAIMGGSGCGK
                     TTLLRLIGGQERAQSGSVRVDGQEVGALGREALYALRRRIGLLFQFGALFTDMTVFDN
                     VAFPLREHTDLPPELIRDLVMMKLHAVGLRGAADLMPGELSGGMARRVALARTIALDP
                     TLLMYDEPFTGLDPISLGVIGQLIRRLNDALGASSVIVTHDVQESLQIVDYIYFISEG
                     RVVAEGTPEAIRNTTDPFVHQFIHAEADGPVPFHYPAGDYATALTGGPR"
     gene            16743..19367
                     /gene="leuS"
                     /locus_tag="PLH85_03375"
     CDS             16743..19367
                     /gene="leuS"
                     /locus_tag="PLH85_03375"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNB63628.1"
                     /translation="MQDKYLPADIERAAQQHWDSTQAFRVTADASRPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFHKMKGYNVLQPMGWDAFGLPAENAAIKNRVPPAKWTY
                     DNIDYMKGQLKSLGFAIDWSRELATCRPEYYKWNQWLFLRMLEKGIAERKTQVVNWDP
                     VDQTVLANEQVIDGKGWRTGAVVEKREIPGYYLKITDYADELLSDLDSLTGWPERVKL
                     MQANWIGKSIGVRFAFGHDIAGDDGKLISDGKLWVFTTRADTIMGVTFCAVAAEHPLA
                     VHAAQDNPALAAFIEKCKHGSVMEADMATMEKEGLPTGLFVTHPLTGEQVEVWVGNYV
                     LMSYGDGAVMGVPAHDERDFGFAKKYGIAIKQVIANGDKAFGLDGWQEWYGAKDGVCV
                     NSGKYDGLGYEAAVDAVATDLAALGVGEKKVQWRLRDWGISRQRYWGCPIPIIHCDAC
                     GSVPVPDDQLPVVLPEDCVPDGSGNPLHKRDDFIHTTCPCCGKPARRETDTMDTFVDS
                     SWYYARFATGNGANTMVDAETDYWMTVDQYIGGIEHAILHLLYSRFWTKVMRDLGLVS
                     YREPFAHLLTQGMVLNHAFFHKPDGGGKNYYWESEIDLLRDSKGQVMGAKLKTDGTVL
                     EHELTTMSKSKNNGVDPQALIEQYGADTARFFMMFAAPPEQTLEWSDAGVEGAYRFLK
                     RVWSFGHVFHSEIRPQLGAERQLATVKLPDDLAALRRELHLLLKQANYDLGKQQFNTV
                     ASATMKMLNALEKAPRGGPLAAEVIEEGLAILLRVLSPLTPHISHSLWHQLGYGDDIL
                     AAAWPEPLDAALAQDAVDLMLQVNGKLRGAVRVAADAPKDVIEAAALAHEATVKFLEG
                     RAPKKVVVVPGRLVNVVG"
     gene            19384..19593
                     /locus_tag="PLH85_03380"
     CDS             19384..19593
                     /locus_tag="PLH85_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63629.1"
                     /translation="MRPVACLIVSLLATTILGACSTQGLYEGIRQENVRRQNAPGRDV
                     SPDPAAPSYDAYNKERERVLKESRP"
     gene            19590..20117
                     /gene="lptE"
                     /locus_tag="PLH85_03385"
     CDS             19590..20117
                     /gene="lptE"
                     /locus_tag="PLH85_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNB63630.1"
                     /translation="MSPLPRSAARRRLLGALLAGPLLASGCGFQLRGPRALPFESIYL
                     GMSEYSELAAAVRRQIRAGGPTRIADRPDDAQVRLEVLSDAREKVILALNTQGRVREY
                     QLRQRFAFRLVTKDGREVIAPNEITLRRDLAFDDSQLLAKEQEEILLYRDMQNDLVQQ
                     LMRRLAAAKMTPAQP"
     gene            20114..21145
                     /gene="holA"
                     /locus_tag="PLH85_03390"
     CDS             20114..21145
                     /gene="holA"
                     /locus_tag="PLH85_03390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNB63631.1"
                     /translation="MILRPEQLAAQLDKPLAPLYVLHGNEPLLVLEAGDALRAAARRQ
                     GYSEREVLVTGQGFRWDSLQSAAGNLSLFGASKLVDLRIPNGKPGRDGGEALQRYARS
                     LDAGVVTLVTLPELDWAARKAAWFTALAEAGVVVELNAPERERLPEWIAQRLARQQQK
                     AQPEALAFIADHVEGNLLAAHQEILKLGLLHPPGELGLDAIRDAVLNVARYNVDALRA
                     ALLDGDAARCARLLEGLRGEGEAPPLVLWALASEVRTLAGLRQGVDSGQPLAALLKAE
                     RVFDERRRQGLQRALPRLGGGALNAALLHAARIDRMIKGLAGGDLWDECLQLCLRLCR
                     SPAPARTAN"
     gene            21156..22433
                     /locus_tag="PLH85_03395"
     CDS             21156..22433
                     /locus_tag="PLH85_03395"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNB63632.1"
                     /translation="MDIKTYMQGVGRQARAASRQVAAASTDAKNTALLAMAAEIRARR
                     AELAAANRRDLEEARAAGLEPAMIDRLTLTEKGIDGMAAGLEQVAALPDPVGEMNELK
                     RRPSGITVGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGKEALHSNQAI
                     AACVRAGLVAASLPEAAIQVVETTDRAAVGELITMPEFVDVIVPRGGKGLIERITREA
                     RVPVIKHLDGNCHVYVDAFADPAKALAIVENSKTQRYGTCNTTESLVVDESVAATQLP
                     ALGRMLAAKGVELRACPVALPILRDAGIPDVQLKAAVESDWYEEFLAPIIAVKVVDGL
                     DAAIDFINTCSSQHTESIVTENHTRAMRFLREVDSASVMINASTRFADGFEYGLGAEI
                     GISTDKIHARGPVGLEGLTSQKWIVFGDGHVRA"
     gene            22554..23141
                     /locus_tag="PLH85_03400"
     CDS             22554..23141
                     /locus_tag="PLH85_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNB63633.1"
                     /translation="MPTPFRHALAALLLSTALGTPSTFAADVAGVHFDDHAAVAGSDT
                     VLNGAGLRTRFMLKVYAIGLYGPKRADTVETFAAVPGPKRIQIVTLRELTAEQFADAL
                     LDGIRKNTSDAEFTRLQPRADEFRAQLLALKSTPSGSQIRIEWLPASGTRLSVGNEVR
                     GRDIPGEDFFRALLRIWLGEKPVDGELRNALLGKA"
     gene            23160..23651
                     /locus_tag="PLH85_03405"
     CDS             23160..23651
                     /locus_tag="PLH85_03405"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNB63634.1"
                     /translation="MEPIFSAIVPAPFGAVGIATAGGCVTEIVFLPPGTAGLAPADPL
                     ADRAFRQIERYFADPDTAFSLPLRPQGSPFRQRVWQAIATIPRGETRCYGELARALGS
                     AARAVGQACGDNPFPLVIPCHRVIAAAGPGGFAHHRGGFLLDTKRWLLAHEAPGPFAE
                     CRD"
     gene            23815..24306
                     /gene="ybaK"
                     /locus_tag="PLH85_03410"
     CDS             23815..24306
                     /gene="ybaK"
                     /locus_tag="PLH85_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNB63635.1"
                     /translation="MSRHDPHPPETPATRFLRQQHIAHSSHLYAYEDHGGTAVSAREL
                     NVPEHAVIKTLVMENERREALIVLMHGDRKVSTKELARQTGHKQIAPCDPAVAQRHTG
                     YMVGGTSPFGTRKALPVFMEQTILELPLIYINGGKRGFLVGIHPHDIIRTLHAKLVHV
                     AIE"
     gene            24439..24825
                     /locus_tag="PLH85_03415"
     CDS             24439..24825
                     /locus_tag="PLH85_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNB63636.1"
                     /translation="MDISGLSLTELRRLQTRIEAEIRRRSDTARKELLKRMQKLAAEH
                     GMSLADVLGQESEKPAAPARKTPPKALAQPARKAAKPASVIKFRHPENTAIGWTGHGR
                     KPQWVIDWLAQGKALEDLRASTEAAA"
     gene            complement(24909..25304)
                     /locus_tag="PLH85_03420"
     CDS             complement(24909..25304)
                     /locus_tag="PLH85_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNB63637.1"
                     /translation="MDFIFIEALRVQARVGIYPRERVAEQTLELNLTFGVPDAAAEHD
                     DIADTIDYAQVIERLRAELAVRQFNLIETLGEFVVKLLFDEFGAPWVKLAIAKMGVMK
                     GVKRVGVFIQRGRNGSAPPEHAPTAGLPG"
     gene            25356..25964
                     /gene="plsY"
                     /locus_tag="PLH85_03425"
     CDS             25356..25964
                     /gene="plsY"
                     /locus_tag="PLH85_03425"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNB63638.1"
                     /translation="MQTLLLLCAAYLIGSVPFAVVVSRAFGMADPRTYGSGNPGATNV
                     LRSGNKLAAALTLLGDAFKGWLAVFLAHLLVPTLGLGPEVVPACALAAFLGHLFPVFL
                     GFRGGKGVATAAGVLIGASGWLGLATLGVWLGVAFGARYSSLAALCAALAAPGLGFLL
                     GLDPYWILAISVMSGLLIWRHSDNIRRLLAGEESRIGSKKKA"
     gene            complement(25995..27020)
                     /gene="tsaD"
                     /locus_tag="PLH85_03430"
     CDS             complement(25995..27020)
                     /gene="tsaD"
                     /locus_tag="PLH85_03430"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNB63639.1"
                     /translation="MRVLGIETSCDETGVALYETGRGLLGHCLHSQIDLHAVYGGVVP
                     ELASRDHIRRLPLLLRELLAKTGTTLADVDAVAYTAGPGLAGALLVGSAFAESLALAL
                     DLPVLPVHHLEGHLLSPLLSATPPAFPFVALLVSGGHTQLMRVTGVGDYHLLGETVDD
                     AAGEAFDKTAKLLGLGYPGGPALATLAQAGRPGRFRLPRPMLHSKDFDFSFSGLKTAV
                     LNAASAPDFTDADKADLAAEFQEAVVDVLVAKSLAALRHTGLRQLVVAGGVGANRRLR
                     DQLDGAAARRKATVFYPELALCTDNGAMIAFAGALRFAAGAPGRTGDGAFAVRPRWSL
                     QEIGTPV"
     gene            27159..27371
                     /gene="rpsU"
                     /locus_tag="PLH85_03435"
     CDS             27159..27371
                     /gene="rpsU"
                     /locus_tag="PLH85_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNB63640.1"
                     /translation="MPGIRVKENEPFEVAIRRFKRTIEKVGLLPELRAREFYEKPTAE
                     RKRKLAAAVKRHHKRLRSQTLPPKMY"
     gene            27639..28085
                     /locus_tag="PLH85_03440"
     CDS             27639..28085
                     /locus_tag="PLH85_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNB63641.1"
                     /translation="MSLKDRINEDMKNAMRAKDAPRLSALRLLLAAIKQRQVDERIEL
                     DDDGVIAVVDKMLKQRRDSVAQYEAAQRFDLASAERFEIDVLTAYKPAGLGADEIAAA
                     IEAAVAATGAAGPADMGKLMAVLKPQLAGKADMAEVSKLVKTRLAN"
     gene            28126..29985
                     /gene="dnaG"
                     /locus_tag="PLH85_03445"
     CDS             28126..29985
                     /gene="dnaG"
                     /locus_tag="PLH85_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNB63642.1"
                     /translation="MIPHAFVQDLLARVDIVEVIERYLPLKKGGVNYFACCPFHGEKS
                     PSFSVSPSKQFYHCFGCGAHGSAIGFLMEYSGLGFVEAVKDLAEQVGMQVPEEAPGAA
                     QERVREQSLTELMTQAARFYKEQLKRSPVAIEYLKGRGLTGEIAARYGLGYAPEGWQS
                     LQAAFADYADPKLLECGLVIENDQGRRYDRFRERIMFPILDQRGHVIGFGGRVLGKGE
                     PKYLNSPETPLFEKGRELYGLVQARGTIRDSGTVLVVEGYMDVVALAQFGVANAVATL
                     GTAATPHHMQKLLRVADRVVFSFDGDAAGRRAAGRALEVCLEHLADNKTVAFLFLPDD
                     HDPDSYVRAFGADAFRQVVDHATPLADFLLAQLRQDIDLATAEGRARLVHEAKPLVTR
                     IGAPLLRLQVIKMLAEAAGFSQNEVEQACGLAAPRRSERRFDDTPPDDGFAARRFSGQ
                     ASSGKPFRGERRPAVPRQPPRQRPATPVETLLKIVVQHPTWSARLPVDLLPADSAEGL
                     ALIAVTDAMSVGDLPTQGVGALLEHFRDTPHAPILARVAVANEETPFEDGTIEPLLND
                     TLNKLQADALSREIERLTAQDRAGGLDPQQRRRLAELLMDKQRLRVRPKVTDS"
     gene            30036..32000
                     /gene="rpoD"
                     /locus_tag="PLH85_03450"
     CDS             30036..32000
                     /gene="rpoD"
                     /locus_tag="PLH85_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNB63643.1"
                     /translation="MTTEKPKTPRKTPAKPRAAKVKDKLAPMIAEAPSAEEAEVRRSR
                     LKSLIALGKERGYLTYAEINDHLPDDLVDAEQIESIISTFNDMGIQVFDVAPAAEDLL
                     MSEVAPTVVDDEQAEAEAEQTLSAVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIA
                     KRIEEGLKHMIQAISACPTTIADILESADRISKDEMRIDELIDGLIDPNAVEDIEAMS
                     DSDELDVDEDAEDEDEDGDDGAGGAAVSASLIKLKADALERFDVLRGIYTKMIAALQK
                     KGPQDKTYVKLQEQISTELMNIRFTARTTERLCDAVRHMVEQARSHERKILHLCVDKA
                     HMPRTHFIKSFPGNETNLDWLKVEIGTNPSNAAVLMRMLPAVVEEQQKLVELQTRIGI
                     PLKDLKDINKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIG
                     LMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQ
                     ILQETGQEPDPATLAAKMEMPEEKIRKILKISKEPISMETPIGDDDDSHLGDFIEDNA
                     TMAPADAALYSSLRDATKEVLDSLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRE
                     RIRQIEAKALRKLRHPSRSERLRSFLDNEA"
     gene            32119..32195
                     /locus_tag="PLH85_03455"
     tRNA            32119..32195
                     /locus_tag="PLH85_03455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32153..32155,aa:Ile,seq:cat)
     gene            complement(32259..32912)
                     /gene="alkB"
                     /locus_tag="PLH85_03460"
     CDS             complement(32259..32912)
                     /gene="alkB"
                     /locus_tag="PLH85_03460"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HNB63644.1"
                     /translation="MTTPDLFEGWTPEDVGPEALSPGAVLLRGFVRAEAAAVLAELDR
                     VLAHAPLRTMQTPGGRSMSVATSNCGARGWVSDAAGYRYAPADPLGGAAWPTMPRLWR
                     TLAERAAAAAGFGGFTPDACLINRYAPGARMGLHQDRDEADFTWPIVSFSFGLPAIFL
                     FGGLRRGDPARRISLFHGDAVVWGGPSRLAFHGVAPVKPGEHPLTGACRINLTFRRA"
     gene            complement(32899..33978)
                     /gene="ada"
                     /locus_tag="PLH85_03465"
     CDS             complement(32899..33978)
                     /gene="ada"
                     /locus_tag="PLH85_03465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HNB63645.1"
                     /translation="MTASTSSPPTAADPRWAAVQARDGTADGHFWYAVTTTGVFCRPS
                     CPSRRPRPEHVRFFDSAAAAREAGFRPCLRCHPDTLADPHVALIATVCRAIQAAETPP
                     SLQALSVLAGLSAGHLQRVFKAATGLSPRAYAAALRAGRVRAGLAAGAPVTHAVLDAG
                     FGSTGRFYARSAQVLGMAPAAWRAGGAGEDIRFAVGACELGALLVAASPRGICALSLG
                     DDPDALVRALQDRFPRACLVGDDPAFATWVSRVAGYLQAPRDGLELPLDLRGTAFQLR
                     VWHALQGIPPGVTISYAELARRVGAPRAVRAVAGACAANPVALAVPCHRVVRSDGSLS
                     GYRWGVERKAALLTREAERGGRDDA"
     gene            complement(34175..34678)
                     /gene="tpx"
                     /locus_tag="PLH85_03470"
     CDS             complement(34175..34678)
                     /gene="tpx"
                     /locus_tag="PLH85_03470"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNB63646.1"
                     /translation="MSSTVTLGGNPVTVAGDLPRVGATAPAFSLVAKDLSEVTLANFA
                     GQRKVLNIFPSIDTPTCATSVRKFNQSASSLPNTAVLCISADLPFAQNRFCGAEGLSN
                     VVTLSTLRGREFLEAYGVALASGPLAGLAARAVVVVDEQDVVRHVELVPEIKNEPDYA
                     AALAALG"
     gene            complement(34813..35727)
                     /locus_tag="PLH85_03475"
     CDS             complement(34813..35727)
                     /locus_tag="PLH85_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB63647.1"
                     /translation="MMDRLQAMEVFVRVAEAGSFAAAADQLDLARSVVTRLVAALEAR
                     LGVKLIARSTRSLRLTSAGQAYLERCREILDLVGVAEGELGGNERAPRGPIRLSVPVS
                     FGIHHLAPLVADFVTTWPDVSLDIDFNDRQVNLVEAGVDMAIRITTQLDPSVVARRLT
                     VSRMLTLAAPEYLARHGRPVRPDDLLDHPCLGYTGTARASWPYRVAGTLQWVPVRGRL
                     QSNNGDALLDAARRGLGICLQPSFIAAAAVQAGLLEVLLADFPGPELGIYAVFPSHRY
                     VPSRVRLLADYLAARIGPVPYWEQGLPA"
     gene            35778..36722
                     /locus_tag="PLH85_03480"
     CDS             35778..36722
                     /locus_tag="PLH85_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNB63648.1"
                     /translation="MKAYSGTQFINPPQESSVILVTGANGRLGRRIVQHLIARRPLRD
                     APALAVSVRQPALAADLAARGVDVRAGDFDQPETLATTFAGVRTLVLVSTDGPSAQRI
                     AQHAAAISAARRAGVQRVLYTSFLDVGTDSPAEFARVHRQTEDALAASGLDATILRNP
                     LYADDLPALVVPGPDGDAFCLAAGNGAVSLVSRDDLARALAAAALAPRLDKRVYELTG
                     SEAVAYAELAARIARVSGRPLRYLPVTVTEQAATLRAAGLSEGLAEGVASLFAAVAEG
                     RLARVTHDFAALVGHPPRSLDCLIREYFAPVTAPRTAS"
     gene            36719..37534
                     /locus_tag="PLH85_03485"
     CDS             36719..37534
                     /locus_tag="PLH85_03485"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNB63649.1"
                     /translation="MSARHPLLFVSHGAPDVLLRPAATVALWADLGRRLPRPRAILVV
                     SAHWAARQPTVSTVSVPATIHDFGGFPEPLYAMEYPAPGAPDVAARVRDALVAAGLPC
                     AEVPDRGLDHGAWIPLMVMYPAADIPVVQLALQPHAGPDWHRRIGTALRGLRDEGVLI
                     LASGAITHNFGWLDWSGGGEPLPQAKAFADWVGAAVARNDRPTLLEYRRQSPLGAAAH
                     PSEEHLLPLFVTLGAADPDEIALRLEPEFTYGGLAMDAYLWQKDSIIPYTPEP"
     gene            37537..38073
                     /locus_tag="PLH85_03490"
     CDS             37537..38073
                     /locus_tag="PLH85_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNB63650.1"
                     /translation="MNTERYSSPAIAAHWLIALLIFAAFPLGLYMHELPLSPTKLKLY
                     SYHKWIGMTVLGLFVLRVLWRLTHRPPAPLPAPAWQQTAAEVTHRLLYVLMALVPLTG
                     WLMSSAKGFQVVYLGVLPIPDLIAKNPDLGEILEEVHESLNWLLFGLVGLHVAAALKH
                     HLIDKDATLRRMLPFGRV"
     gene            38102..38680
                     /locus_tag="PLH85_03495"
     CDS             38102..38680
                     /locus_tag="PLH85_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB63651.1"
                     /translation="MKTLRPTLTLALLAGLTTGAAHAADFTQVLTDKSSLAFTSKQMG
                     VPVDGLFRKFSAQLAFDPAKPATASTVLEIDLASIDAGSKDANEEVVGKQWFNVKAFP
                     TARFVSTAVKAVGGGRYEVSGKMTIKGQTHDVVAPFTFRQDGANGVFDGGFVLKRMDF
                     GIGEGPWSDVSAVANEIQIKFHVVAAPAPAKK"
     gene            38751..39317
                     /locus_tag="PLH85_03500"
     CDS             38751..39317
                     /locus_tag="PLH85_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB63652.1"
                     /translation="MKKLTALILAAGLSTAALAAPESYTIDGSHTFPRFEYSHFGYST
                     QLSRFDKTTGTIVLDRAAKSGTVDVTVDATSVDTGFPLLNQHIQGEDFFDTAKYPTIT
                     YKSTKVNFDGDKVASVEGNLTVKGVTKPVTLTVTSFHCMPHPMLKKDACGANAVAKIK
                     RSEFNAGKYAPYVGDDVTLTIAVEAYKP"
     gene            39330..39617
                     /locus_tag="PLH85_03505"
     CDS             39330..39617
                     /locus_tag="PLH85_03505"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNB63653.1"
                     /translation="MAPGCAAPRRLHGSLPMTEPQIAQYASYTVPVEAGREYTWCACG
                     RSATQPFCDGRHAGSGIAPKTFTPLRSGLAYLCGCKHSRRAPFCDGTHDTL"
     gene            complement(39614..42541)
                     /locus_tag="PLH85_03510"
     CDS             complement(39614..42541)
                     /locus_tag="PLH85_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNB63654.1"
                     /translation="MPDKGARGRGARWCLPLHIHISTLFFLLVLMAGGSIAWVSYTRS
                     ADMMERAAADLLGRISRQIAAETETLLKPVGASVRLLARSPVTRAGTLETRREQLASF
                     REALLASPSASAFYIGYEDRDFFLLLRLANRSDRDLMGAPPGAAYLLQSIDHGSGRFV
                     FLDEDLATLRSDPRPDYGTAYDPTRRSWYGQALAGPEPIQTEPYLFASTRKLGVTVAR
                     HTDTGRSVVGADIRLETLDESLRRQRITPGSQLVLFDRDLGVLAYTGKDWLPPPQAGE
                     ARRPDLTELGGGVLKALAQRLPQDGETEPLGSFRAAGRDWHGAVAQVQWLGGQPVYLG
                     VVIPDEELLVEARGIRNQSISVTLLVMLLALPFTYLAARLVSRPIRDLAGETAAIRRF
                     DFSSPVRTRSVVREVDNLATDLGSMKGAIRRFLDIASAMAEESSFERLLPRLVDETVA
                     VVGARAGVLYLAAHGGEALDAVVLRDADGASHPLEQAPAGATMAGLVPAGARSGVGRL
                     TLQTAAGAASQTLFGLLGAEDLAYLAVPLTDRKQARLGLLVLWFDAPPDRDLVHFVEA
                     LSGSAAMSVETRALIRAQKELFEAFIQLIAGAIDAKSPYTGGHCARVPELTKMLAQAA
                     CNSADGPFRDFRLGEEDWEAVHIAAWLHDCGKVTTPEYVVDKATKLETLYDRIHEIRM
                     RFEVLKRDATIAYWQARAEGADEAAAKGRLVETHAALDREFAFVAACNEGAESMAPED
                     VARLQQIARRTWLRTLDDRLGLSPEERRRRADAGPVRLPVTEPLLADKPEHRYERGAM
                     DLLPADNPWGFRLKVPALLYNRGELTNLSITRGTLTDEDRYKINEHIVYTIRMLSELP
                     FPRHLRAVPEIAGGHHEKMDGSGYPRGLKGGEMSLVARMMAIADIFEALTAVDRPYKT
                     GKRLSEAVSIMAGMRDRGHIDPDLFALFLRSGVYRRYAERFMQRKCVDEVDVDAMLAE
                     VTA"
     gene            complement(42553..43575)
                     /locus_tag="PLH85_03515"
     CDS             complement(42553..43575)
                     /locus_tag="PLH85_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB63655.1"
                     /translation="MNAPPPDKGTISIHFVQEALEGVVQRGLDAEDLLNRAGISPELL
                     NAPQARVSSAHFGALWHLIAQALDDEFFGMDSHPMRVGSFTLLCHALIHCETLEKALR
                     RALRFLRLVLDDLSGTLVRDGDRAYLVIADRCSAPRRAFAYGTYLIILHGLACWLIGR
                     RIPLQEARFRCAEPAFSGEWRVLFSPDLRFDQAETAIVFPADYLDMANVQNERTAKVF
                     LRSAPANFLVKYRNSEGLTARIRRLLRRLPPEAWPDFDTLARQLHSSASSLRRHLTDE
                     GQSYQSIKDDLRCDLAISQLSHSDKSVLDIAHELGFAEASAFHRAFKKWTGARPGEYR
                     RALAGH"
     gene            43806..44570
                     /locus_tag="PLH85_03520"
     CDS             43806..44570
                     /locus_tag="PLH85_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB63656.1"
                     /translation="MEIQNNVFVVTGGGSGLGAATARQIVARGGKVVLADVNAQAGEA
                     LAAELGAAARFVATDVTDEASAQAAFAAATAMGPLRGLVNCAGVAPAEKVVGKEGAHK
                     LASFTRTITINLIGSFNMIRLAAEIMSKSEPDEGGERGVIVSTASVAAFDGQLGQAAY
                     AASKGGVVAMTLPIARELSRAGIRVMTIAPGIMETPMLLGMPADVQDALGKMVPFPSR
                     MGKPAEFAALVEHILGNRYLNGEVIRLDGAIRMAAK"
     gene            44626..45816
                     /locus_tag="PLH85_03525"
     CDS             44626..45816
                     /locus_tag="PLH85_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNB63657.1"
                     /translation="MSTANDPVVIVSAARTPMGGFQGDFSSLTAAQLGSVAIKAAVQR
                     AGISAEAVEEVIFGCVLPAGQGQAPARQAALGAGLSLSAGCTTINKMCGSGMKATMLA
                     HDLLLAGTNEVMVAGGMESMTNAPYVLPKARGGYRLGHGQIFDHMFLDGLEDAYEKGR
                     LMGTFAEDCAGLYQFTREQQDAYAVASLTRAQAAIRDGAFAWEIEPVTLAGRKGEILI
                     KDDEQPPKANLEKIPSLKPAFRKDGTVTPANSSSISDGAAALVLMRASSAERMGLKPL
                     ARIVAHTTHAHQPSLFATAPVGAMQKLLAKTGWSVADVDLWEINEAFAVVAMAAIHDL
                     QLDHAKVNVHGGACALGHPIGASGARILVTLLGALRKTGGKRGVASLCIGGGEATALA
                     LEML"
     gene            46176..47312
                     /locus_tag="PLH85_03530"
     CDS             46176..47312
                     /locus_tag="PLH85_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB63658.1"
                     /translation="MILTQEQEMIRDSMRAFSQERLAPFAGEWDKHHTFPAQALKELG
                     ELGALGMVVPEEWGGAGMDYMSLVLTLEEIAAGDGATSTIVSVQNSLACGITMKYGTD
                     AQKEEWLKPLARGEKLGCFCLTEPHTGSDAAAITTRAEKDGDHFVLNGVKQFITTGKH
                     AHMAIVFAVTDKAAGKKGISCFLVPTATPGFIVGRTEEKMGQHASDTVQIIFENCRVP
                     ASSLLGKEGEGYRIALSNLEAGRIGIAAQSIGMARAAFEAAVRYARERVTFGVPIIEH
                     QAVNFKLADMNTLLDAARLMVWRAAQLKDAGKPCLKEASMAKMFASEAAEKIASDAIQ
                     IHGGVGYTSDFPVERIYRDVRICQIYEGANDIQRLVIGRSIAAE"
     gene            47675..48820
                     /locus_tag="PLH85_03535"
     CDS             47675..48820
                     /locus_tag="PLH85_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNB63659.1"
                     /translation="MGPLHGIKVIEFAALGPCPMAAMILADLGAEVVRIERKPAPGAK
                     PASDLFDPAIDILNRSRRVVTLDLKKPEALAAARQLIAGADVLVEGFRPGVMERLGLG
                     PDETLAANPRLVYGRMTGWGQHGPLAQAAGHDINYLSLSGALHAIGEPGGKPVVPLNL
                     VADCGGGAMLLVVGVLAAVLEARQSGRGQVVDAAMTDGSALLMTMMYTFKAMGQWTQA
                     RGSNLLDGGAPFYDTYRCADGKYLSIGPIEPPFYALFLEKAGLTDPDFRQQWDRARWP
                     ELKQRVAAHLATRPRDAWCALLEGTDACVAPVLDMDEAPAHPHNRARGTFIEVNGVVQ
                     PAPAPRFSRSTPAAPTPPTPGAPGEDVLADWGLSPDAIATLKAVGGL"
     gene            48845..49621
                     /locus_tag="PLH85_03540"
     CDS             48845..49621
                     /locus_tag="PLH85_03540"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNB63660.1"
                     /translation="MSYENILSEVRGKVGLITLHRPKALNALNDDLVNEVRQALDAFE
                     ADEGIGCIVITGSEKAFAAGADIGAMASYSYMDAYKGDYITRNWERVKTCRKPVIAAV
                     AGYALGGGCELAMMCDMIFAADTARFGQPEIKLGTLPGAGGTQRLPRAVGKAKAMDLC
                     LTARLMDAAEAEKAGLVARVIPADRLLEETLAAAEQIAGYSLPVVMMIKESINRAYES
                     ALNEGLLFERRLFHASFGLEDQKEGMAAFVEKRKADFKNR"
     gene            complement(49731..50243)
                     /locus_tag="PLH85_03545"
     CDS             complement(49731..50243)
                     /locus_tag="PLH85_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB63661.1"
                     /translation="MAVGPMVWSRLVRLTHWGVAAAVLFDLFNETGPLHRATGYGAVA
                     LVLLRLAYGACMPAGDPAALSRPGLTAIGVHLREMGRGHLSPVAGHNPLGQWAAYLMW
                     ALVLALGLTGWMSRLDAFWGDDGLIELHEALADGLMACVALHLMAVALVSRLQGESLL
                     RAMLTGRRRG"
     gene            complement(50257..50517)
                     /locus_tag="PLH85_03550"
     CDS             complement(50257..50517)
                     /locus_tag="PLH85_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNB63662.1"
                     /translation="MRALILAILTLSPLAAFAGARCEAHPKSEWMSEENAKARLVAEG
                     YQIRKFKVDGNCYEIYGKNREGKKVEIYFDTKTLAIVKAEIE"
BASE COUNT         8738 a        18257 c        16332 g         7565 t
ORIGIN      
        1 aacgtctgat tcggcacagg gcgtaccatc tcagcaacag cggggccttt caccatgccg
       61 gacgacacgt cttattacgt gtatgcgctg aaagacccac gcaggtcccc ggcgcttccc
      121 ttctacatcg gcaagggcac cggcacgcgc gcccacgacc acctgatcaa gccggatggc
      181 acccgcaaag gcgcccgcat ccgcgagatc gtcgattccg gcggtcacgt gctggttacg
      241 cggctggtcg atgcgctgac cgagcagcaa gcgctctcgc ttgaagccga attgatcgcc
      301 gcattcggca cactggatac aggtgggtta ctgaccaata cagtcgtgcc ctcgggcctt
      361 tcaagaatga cgaagcccgc cattgttgtc ccatccggcg tgcgggaaaa ggcgcaactc
      421 gggctcgccc tgctcaagga cgccatcctc gaaatggcca aggcgaatcc ccggggcatc
      481 accaattccg acgccgcgag cctgctggga ttgcgcagcg actatggcgg cggatcaaag
      541 gactatctgt cttacagtgt cattggcctg ctcatgcggg aaggcaaagt ccagcgcgat
      601 ggggcttcgc gacgacacat cgcccgggtc gagtgagggc cccgcgtcag gccgacccgc
      661 tctccacacc cacctcgcgc ttgaccacga accgcgccag gaacacaccg gcttcgtaga
      721 gcaggcacat ggggatcgcc agcatcagct gcgacaccac gtccggcggg gtgaccacgg
      781 ctgcgacaat gaaggcgccg acgatcacgt aggggcggac ttccttcagc ttggccacat
      841 ccaccaggcc gatcttgacc agcagaatga cgatcaccgg cacctcgaag gtgatgccga
      901 aggcgacgaa catggacatc acgaaggcca ggtactgctc gatgtccggc gccggggtga
      961 tgctcttggg cgcgaactgg gcgatgaact tgaagaccgt gccgaacacg aagaagtaac
     1021 agaaggccat gcccgccagg aacagcaccg tgctgccaat caccaggggc aggcccaggc
     1081 gcttttcgtg ggcgtagagc cccggcgcga tgaaagccca ggcctggtag agcacaaagg
     1141 gcaacgcgat gacgaaggcc accagcaggg tcaccttgac cggcacgaag aagggcgtga
     1201 ccacgccggt ggcgatcatg cgggtgcctt ccggcaggtt ggccatcatc ggcagggcga
     1261 gcaggtcgta gatgtcgccg gcccagggca tcaggcacac aaagaccagc accaccgcca
     1321 cgacggcccg cagcaggcgg tcgcgcagtt cgaccagatg ggagatgaag ctttcttcct
     1381 gaggggcatt catgattgtt cggccttggc agcgggggcg gcttccgcag cggcaaagtc
     1441 gaagccgagc tgacggtcat cgggcatggc ggcgtcgctg ccgtccggct gcgcctcggc
     1501 aaagaccggc gcaccggcat gctgctcggc ctcggcaagc tggcggtcga ggcgctgcgc
     1561 cgcggcatcc gcatcggtca catctgccga aggggaggca gacggggcag acggcgcttc
     1621 ggactgcgcg tccggctcgg cagacggcgc ggctgcctgg agttcccgct ccagctcggc
     1681 caccgacgac gcagcgccct gcaccgcgga atccacttcg cccttgaaat gcgccatctc
     1741 gtcgcggacg gaggtttcca tctgcagggc agcctggcgc gcttcgtcct gcaggcgctt
     1801 gagctcgtcg atctgcattt cccggtggat gtctgacttc acatcggcga cgtaacgctg
     1861 cagacggccg agcaggtgcc cggtggtacg cgccaccttg ggcagacgct ccggcccgag
     1921 gacgatcagc gcgacaacgc caatgacgat gagctcagaa aaaccgatat caaacatccg
     1981 gacagcccga caagacggga ccgcggcacg cgcgtgacgc ctgcatcagc cccggtttac
     2041 aaaaacaaaa ggggccgcct gacgcgcccc ttgctgcgcc tgctccgcga cggagatcag
     2101 gacttgttct tctcacgggc ctcggcgtcg atcgtgcggc cctcggcgcc cgcaatcttc
     2161 tgggggtcgc cggcagcacc ttcaccttcg cgcatgccct gtttgaagcc cttgacggca
     2221 ccgcccagat cctcgccgat gttgcgcagc ttcttggtgc cgaacacgag catcacgatc
     2281 accagcacga tcagccagtg ccaaatactg aaagaaccca ttcccttctc cttaaatgcg
     2341 ccggagcatc ggtccgaccg gctcctgtcc gcccacgatg tgcatgtgca cgtgcatcac
     2401 ttcctgtccg ccgacagggc cggtgttgat gatggaccgg aacccgttcg gactgccttg
     2461 atcggcagca atccgatgag ccaccgccag catccgcccc agcacgggca cgtccgcctc
     2521 ggaggcgtgg gccagcgagg tgatgtgatg cttggggatc accagcacat gcacgggacg
     2581 ttgcgggtgg atgtccttga aggccagcac ctcgtcgtct tcgtacacct tggcagccgg
     2641 gatctcgccc cgcacgatgc ggcagaacag acactcctgc atggggacag ttccttactt
     2701 ggcggtacgc gccttctttt cgtcgatgcc cgagacacct tcgcggcggc ggaactcggc
     2761 cagcacgtcg tcgacggaca gaccgaagtg ggcgagcagg atcatggtgt ggaaccacac
     2821 gtcggtcact tcccagacca cgtggaggag gtccttgtcc ttggccgcca tgatggtctc
     2881 ggcggcctct tcggcgacct tcttgcagat ggcgtcggtg cccttgtgga acaggcccga
     2941 cacataggag gactcgggat ccgcttcctt gcgttcggcc agggtctggg cgacgcggtg
     3001 cagcacttcg atgtcgatca tttgtagatg tccttcgggt ctttgagaac cgggtcgacg
     3061 gcctgccagc ggccgtccgc gaagtacttc tggaaaaagc agctgcggcg cccggtatga
     3121 caggccatgc cgccaatctg ctcgatcttc aggagtacca cgtcgttgtc acagtcaata
     3181 cgcagctcgc ggaccttctg gatgtggccg gactcttcgc ccttgtgcca cagcctgccg
     3241 cgggagcgcg agaagtatac cgcctcgccg gtggccaccg tgcgggccag ggcgtcgcga
     3301 ttcatccacg cgaacatgag cacctcgccg gtctcggcgt cctgggcgat ggcgggcacg
     3361 aggccctggg catcccaggc gacggcatcg agccacgctt cgctcacagg cgtacctcga
     3421 tgccacgggc gcgcatgagc tccttggcct cgcgcacggt gtggtcgccg aagtggaaga
     3481 tgctggcggc cagcacggca tcggcgcggc cgaggctgac gccgtcggcc agatggtcca
     3541 gattgcccac gccaccgctg gcgatgacgg ggatgttgac cgcgtccgac acggcacggg
     3601 tcaggcccag gtcgaagccg ttcttggtgc cgtcccggtc catgctggtg aggaggatct
     3661 cgccggcgcc cagggattcc accttgcgcg cccattccac ggcgtccagt ccggtccggt
     3721 tgcggccacc gtgggtgaag acttcccacc ggcccggcgc cacctgcttg gcgtcgatgg
     3781 ccaccacgat gcactggctg cccaccttgc cggaggcctc agccaccacc tgcggattat
     3841 tgaccgcggc ggtgttgatg ctgaccttgt cggcccccgc attgaggagc cggcgcacgt
     3901 cgtctacggt gcgcacaccg ccgcccacgg tgaggggaat gaagacctgc tcggccacct
     3961 gctccaccac gtggaggatg atgttacggt cgtcggaact ggccgtgatg tcgaggaagg
     4021 taatctcgtc ggcgccctgt tcgtcgtagc ggcgcgcgac ttcgaccgga tcgccggcat
     4081 cccgcagttc gacgaaattg acgcccttga cgacacgccc ggcgtttacg tcgagacagg
     4141 ggataatgcg cttggcgagc atgggatcag gccgctccgg agagtgcgtc ggcgcgggcc
     4201 tgggcggcgg agaaatccag cgtgccctcg tagatcgcgc ggccggtgat ggcaccggtg
     4261 atgccttcgg attccacggc gcacagggct tccacgtccg ccatggcggc gatgccgccg
     4321 ctggcgatga ccgggatgcg cagcgcctgg gccagcttga cggtggcctc cacgttgacg
     4381 ccgctgagca tgccgtcgcg gccgatgtcg gtgtagatca caccttcgac gccgtaatcc
     4441 tcgaacttgc gggcaaggtc gaccacgtca tgaccggtga gcttggacca gccatccacc
     4501 gccaccttgc cgtccttggc gtcgagcccg acgaggatgt ggccggcaaa ggcgctgcac
     4561 gcatcgtgca ggaaacccgg attcttgact gccgcagtgc cgatgatcac ataggacaga
     4621 ccggcgtcga ggtagcgctc gatggtgtcc aggtcgcgga tgccgccccc cagctggacg
     4681 gggatctcgt cgcccacctc ggccaggatg gagcggatcg ccgcctcgtt cttgggcttg
     4741 ccggcgaagg cgccgttcag gtccacgaga tgcaagcggc gcgccccctg gtcgagccag
     4801 tgtcgggcca tggcgcgggg atcttcggaa aacaccgtgg cggcgtccat ctcgccttgt
     4861 ttcaggcgca cgcagtgacc atccttcagg tcaatagcgg gaatcagcag catagggagt
     4921 gcggaggagg aaggggatcg ggacggggga gacgcccagg cgatgcgggc gggccatcag
     4981 ggcttccagg acatgaagtt cgacaggagt tgcaggcccg cttgcgcgct tttttccgga
     5041 tggaactgga cggcaaagat attagcctgc gctaccgccc cggtaaaggg gatgccgtac
     5101 tcggtggtgg cggcgatcac cgccgggtcg gccggatcga cgtaatagct gtgcacgtaa
     5161 taaaaacggc tgccgtccgg aatgcctgcc cacaggggat gggcctgctt ctgccagacc
     5221 tcgttccatc ccatgtgggg caccttgagg cggccaccgt cgggcgcggt catccgcgca
     5281 tcggacggaa agcgccgcac gtgaccacga aacaggccca gcccgggcac gtcaccctcg
     5341 tcgctgtgat cgaagagcat ctgctggccg atgcagatgc ccagaaaggg cttgctgcgc
     5401 gccgcctcca gcaccgccgg gcgcaggccg cgggcgtcca gttcacgcat gcagtcgggc
     5461 atcgcaccct ggccggggaa gaccacccgc tccgccgcca gcaccgcggc cggatcagcg
     5521 gtcaccacca ccgttgcacc ggtggccaca tgctcgatgg ccttggccac cgagcgcagg
     5581 ttgcccatgc cgtaatcgat gatcgccacg ctgctcatgt cacagggctc ccttggtgga
     5641 gggcaccacg ccggcctggc gtggatcggg cgtcaccgcc atgcgcaggg cgcgcccgaa
     5701 ggctttgaag acggtttcgc attgatggtg ggcattgcgg cccttgaggt tgtcgacatg
     5761 caggctcacg cccgcatggt tgacgaagcc ctggaagaat tcgtagacga gctgggtatc
     5821 cagcgcgcca atggccgccg ccgtgaaggg cacgtcgaat tccagccccg gccggccgga
     5881 caggtccacc accacccggg acagggcctc atcgaggggc acataggcat gaccgtagcg
     5941 ggtcaggccc ttcttgtcgc cgatggcccg ggcgaaggcc tgaccgaggg cgatgcccac
     6001 gtcctccacc gtgtggtggc cgtcgatgtg caggtcgccc ttcgccgcga tctccagatc
     6061 aaagcagccg tggcgggcga tctggtcgag catgtggtcg aagaagccga taccggtatc
     6121 gagccggctc tggccggagc cgtccagatc cagacggacg cggatctgcg tttcgagggt
     6181 gtcgcgggag atttcagcct ggcgcatgcg ctgtgttcca gaaaatcgtt gaaagccggt
     6241 gaatacgaac ggtcatgata gcatttccca ttttcaccgt ccctggcggc gcccccggac
     6301 gccgcgccga tctgtcatga gccgcttctg gagccccgtc gtccacgacc tgacgcccta
     6361 cgtcccgggc gagcagccca agctcgccaa cctggtcaag ctcaacacca acgaaaatcc
     6421 ctatccgccc agcccgcggg tggtggacgc catccgcgcc gagctgggcg aggacggcga
     6481 ccgcctcaag ctctacccgg atcccaccgg aagcgccctg aaggccgccg tgagccgcca
     6541 attcggcgtg ccggcggagt gggtgttcgt cggcaacagc tcggacgaag tgctcgccca
     6601 taccttccag ggcctgctca agcacgacgc gccgctgctc ttcccggaca tcacgtatag
     6661 cttctatccg gtgtattgcg gcctctacgg catccccttc gagaccgtgc cgctggccga
     6721 ggacttctcg atccgcgtgg acgattacct cgtccgcgga cccgccgcgg tcggcggcgt
     6781 gatcttcccc aatcccaacg cgccgacggg gcgcctgctg cccgtcgccg agatcgcccg
     6841 cctcgccgcc ggcctgcccg acagcccggt ggtggtggac gaggcctacg tggatttcgg
     6901 cgggcacgac gcgattccca gcgcggtggc gctggtggcc gagtacccca atatcctggt
     6961 cacccagact ctctccaagt cccgttccct ggccgggctg cgggtcggct tcgcgatcgg
     7021 ccagccggaa ctggtggaag ccctcacgcg ggtcaaggac agcttcaact cctacccgct
     7081 ggaccgcctc gccctggtcg gcgcctgcgc agcgctggac gacacggagt ggttccattg
     7141 cacccgccat caggtcatcg ccagccgcga ggccctcagc gcgtccctga ccaacctggg
     7201 cttcgaggtc ctgccctctg ccgccaactt cgtcttcgcc cgccacccgg cccacgacgc
     7261 ggccctgctg tccgccgccc tgcgcgcgcg cagcatcatc gtgcgccact tccgtgcccc
     7321 gcgcatcgac cagttcctgc gcatcacggt gggcaccgat gcccagtgcg cgcagctggt
     7381 gacggccctg ggcgagatcc tcggctgacc gcccgccggc gaacaccgca acgaaaaacc
     7441 gcgcccgaag gcgcggtttt ttatgggcag acgcaaatgg acggccggct cagtccttca
     7501 gccgcatctc cgccgaacgg gcatgggcct ggaggccttc gccgcgggcg agcaccgagg
     7561 cgatgcggcc cagggtctgg gcgccggcct cggagacttc gatgaggctg gtgcgcttct
     7621 gaaagtcgta caccccgagc gggctcgaaa agcgcgcgct gcgcatagtg ggcagcacgt
     7681 ggttggggcc ggcacagtag tcgcccaggg cctcgacgga gtggtggccg atgaagatcg
     7741 cgcccgcgtg gcggatgcgc tcgacccacg ctcccggctc ggcgagggac agctccaggt
     7801 gctccggcgc aatgcggttg gcgatgtcgc aggcctcggc cagatcgcgc acatggatca
     7861 gcgcgccgcg gttcttcagg gaggtggcga tggtgtcgcg acggggcatg tcgggcagca
     7921 ggcggacgat gctcgccgcc acgcgggcga tgaagtccgc gtcggtgcac agcaggatgg
     7981 actgggccag ctcgtcatgc tcggcctggg cgaagagatc catcgccacc cagtccggat
     8041 cgccggagcc gtcggagatg accagcactt cggacggtcc ggccaccatg tcgatgccca
     8101 cggtgccaaa gacgcggcgc ttggcgctgg ccacgtaggc gttgccgggg ccgacgatct
     8161 tgtccacctg ggggatgctc ccggtgccat aggccagggc ggccaccgcc tgggcgccgc
     8221 cgatggtgaa cacccgatcc acgccggtga tggcagcagc agcgaggacc agcgggttct
     8281 tctcgccccg cggtgtgggc accaccatga tcagctcgcc caccccggcc accttggcgg
     8341 ggatggcgtt catcagcacg gagctgggat aggcggcacg cccgcccggc acgtagagtc
     8401 cgacccggtc cagcggcgtg acctgctggc ccaggcgggt gccgcgcatg tcgggcgtgt
     8461 cctcgacgta gctccaggat tcctgcacct gacgcaggtg gtagcggcgc acccgctcgg
     8521 cagcagcttc gagggccgtg cgctgctcgg caggcaatcc ggcgagggcg gccgccagct
     8581 cggcgcgggg gagttccagc gcggccatgt cggcggcgtc cacgtggtcg aagcgacggg
     8641 tgaattccac caccgcggca tcgccatccc gtcgcaccgc ggccagtatg tcggccacgg
     8701 ccgcctcgat ggcggcgtcg gtgctgccct cgaaggccag cagggcgtcc agggtggcgg
     8761 cgaagccggc gtcggaagac gccaggcggc ggatcggttc gctcatggct tgacggctcc
     8821 ggcaaaggcc tccagcaccg gctggatgag ttcgcgtttg agcttgaggg aggcctgatt
     8881 gacgacgagg cgggagctga tgggcatgat gtcctccacc tccaccagat tgttggcgcg
     8941 cagggtgccg ccggtggata ccagatcaac gatggcgtcc gccaggccca ccagcggcgc
     9001 cagttccatg gagccgtaga gcttgatcag atccacgtgc acgcccttgg cggcaaagtg
     9061 ctcgcgggcg gtcttgacgt acttggtggc cacgcggatg cgcgcgccgc gcttgacggc
     9121 ggcggcgtag tcgaaatcct tgggtgtcgc cacgcacagg cggcacttgg cgatgttcag
     9181 gtcgaggggc tggtacaggc caacgccgcc gtgttcgatg agcacgtcct tgccggcgat
     9241 gcccaggtcc gccgcgccgt attgcacata ggtgggcgtg tcggtggcgc ggacgatgac
     9301 gaggcgcacg tccggccggt tggtgccgat gatgagcttg cgcgacgact cggggttgtc
     9361 ggtgggcacg atgccggccg cggcgagcag cggcagggtt tcgtcgaaga tgcggccctt
     9421 ggagagggcg agagtgatgc cgttcacggt cgtgttctgc gtggcaaagg agcaattcta
     9481 gccgagccgc cacagcggcc cggcgatttc cgcggaatgc ggcctacttg agtcgggtga
     9541 cgcgggcgcc cagggccgca agcttggctt cgagccgctc gtagccccgg tccaggtggt
     9601 agatgcgctc aatggtggtc tcgccttccg ccaccagacc ggcgatcacc aggctggccg
     9661 atgcgcgcag gtcggtggcc atcaccgtgg cgccctgcag accggccacg cccttcacca
     9721 ctgccgtgtt gccgtcgatg cggatgtcgg cgccgaggcg ctggagctcc accgcgtgca
     9781 tgaagcggtt ctcgaagatg gtctcgcgga tcaccgcagt gccgtcggcc accgtgttga
     9841 gggccatgaa ctgggcctgc atgtcggtgg gaaaagccgg atagggcgcg gtacgcaggc
     9901 tcaccgcggt gaggcgggcc ggcgccttga ggcggatcgc gtcgcgctcg gcggtgatct
     9961 cgcaaccggc gtccatcagc ttgtccacca ccgcgtccag gtagctggtg gaggtatggg
    10021 tcaggcgcac ctcgccgccg gtgacggccg cggcgcacag gtaggtgccg gtctcgatgc
    10081 ggtccggcat cacccgatgg gtggcgccgt ggagggccgg cacgccctgg atgcggatca
    10141 cgtcaccgcc tgcgccggag atgcgcgcgc ccatggccac caggcagttg gccaggtcca
    10201 ccacttcggg ctcgcgggcg gcgttctcga tgacggtctc gccgtcggcc agacaggcgg
    10261 ccatcagcag gttttcggtg ccggtgacgg tgaccatgtc ggtgaagatc cgcgcccctt
    10321 tcaggcgggg cgagcgggca ttgacgtagc cgtgctccac cttgacgtcc gcccccatgg
    10381 cctgcaggcc cttgatgtgc tggtccaccg gccgcgcgcc gatggcgcag ccgccgggca
    10441 ggctcacccg cgcctcgcca aagcgcgcca gcagcggccc cagcacgagg atggaggcac
    10501 gcatggtctt gaccatctcg taggacgcca ccgggttgtt caccccgccg gcgtcgaggg
    10561 tcaccgtgtc gccatcgcgg ctgaccttca cgcccatctg ttcgagcagg cgcagcaggg
    10621 tgccgatgtc gttgaggcgc ggtacgtggg tgaaggtcac cggctccttg gtcagcaggg
    10681 ccgcacacag gataggcagg gccgcgttct tggcgccgga aatggcgatc tcgcccgaca
    10741 ggcgggcgcc gccttcgatc aacagtttat ccatgcatca ggggcgttca gcccagttcc
    10801 ttctgaaagt cggcccattg ggcgggcgtg taggtggtga gctggagggc gtggatctcg
    10861 ttgcccatca gcttgccgag ggtggcatag acggcctggt gctggcgcac cttgggtttg
    10921 ccctcgaagg cggcactcac cacgatgcca gagaagtggg tgccgtcgtc cccttccacc
    10981 tgcaggaatt cgcagtccag tccggccccg atgagccgtt cgatttcact tgccagaaac
    11041 atgttggatc ctcaggcccg cagcttgtat cccctggcca gcatctgcag ggtggccacc
    11101 gcgagcacga tgaaacagac cgaaaccact ccgacgctcg tccacggcga gacgtcggag
    11161 accccgaaaa atccgtaacg gaacccgtcg atcatgaaga aaaacgggtt gaaatgcgag
    11221 accccctgcc agaaacccgg cagcgagtgg atggagtaga agacgccgga cagcatggtg
    11281 aggggcatga tcaggaagtt ctggaaggcg gcgagctggt cgaacttgtc cgcccagatt
    11341 ccggcgatca cccccagcgt ggccaggaag gcgccgccga tcagcgtgaa ggccacaatc
    11401 cacccgggtg ccgccacgcc gggcatcgca aagggcagcg acacgagcag cacgcccagc
    11461 ccgcacaggg cgccgcgcag gatggcggcc agcacgtagg cggcgtagaa ctcccgatag
    11521 gagatgggcg gcagcagcac gaagacgatg ttgcccgtga tcttgctctg aatgagggag
    11581 gacgagctgt tggcgaaggc gttctgcagc accgtcatca tcaccaggcc gggcaccagg
    11641 aaggctgtgt agctgacgcc gggatagggc tggacgtggt cttccagcac gtgcccgaag
    11701 atcagcaggt acagcacggc ggtcagcacc ggcgacagca cggtctggaa acccaccttc
    11761 cagaagcgca ggcattcctt atagaacagg gtcttgaagc cttccatggc tcagctttcc
    11821 ttcatcacgc cgacgaagac ctgctccagg tcgggcttgc cgaggaccat gtcggcgatc
    11881 tgtgcacccg cgccgcgcag ggcgtcgagg agcgcaccca catcgtcgaa gcgctcgaag
    11941 ccgagcacca gatcgagacc gtcctggcgc accaccgccg cggcgagggc cgggggcaga
    12001 ctcaccggcg cggccaggcg cacctgcagg gtgtggttcg caaatcgcgc caggagattg
    12061 gcggtggtat ccagggcgac gatgcggccg gccttgagca tggcgatacg cccgcacagg
    12121 gtctccgcct cttccagata gtgggtggtg agcaccaccg tgtggccgtc ccggttgagc
    12181 ttgcggatga actgccacag tccctggcgc agctccacgt ccacgccggc ggtgggttcg
    12241 tccagcacga tcaccggcgg acgatgcacc agggcctggg ccaccagcgc ccgccgcttc
    12301 atgccgccgg agagcatgcg catgttggcg tgggccttgg gcgtcaggtc caggtgatgc
    12361 aggatctcgt cgatccagtc gtcgttgcgc ttgagtccga agtagccgga ctggatgcgc
    12421 agcatctcgc gcaccgagaa gaacggatcg aagaccagtt cctggggcac cacccccagg
    12481 gccagacggg cggcacggta gtcgcggacc acgtcgtgac ccatcacctc cagcgtgccg
    12541 gcgtcggcgc gggcaagccc ggcgagggcg gagatgaggg tggtcttgcc ggcgccgttg
    12601 gggccgagca aaccgaagaa ctcgccctgg gcgacctcca ggtccacccc ggccagggcc
    12661 tgcagggcac ggaaacgctt ggtgacgccg acggcgcgga tcgcaggaat catgggttcg
    12721 gagcgccccg gctcaggccg ccgggggcag gaggccatcc acgtcgtaga gggacgcgag
    12781 gctgacgagg gccgtcgggg cgttgacgag gctgaggcga tggccggcgc tgcgcgcagc
    12841 ccgcagccag gcgaagagca gcgccagggc ggcggaatcc acgcgcgtca ccgcggagag
    12901 gtccgcgacg caatctcccg ccgcgaccgc cgcccgacct gcctcgagca ggccgctgac
    12961 ggtggtgagg gtgagcgcgc cggagaggcg cagacagtcg ccgtcctgga cgatcatttc
    13021 ttgggcgccg cctcaagctg cttgttcttg tccttgaggg acttgatgag gccgtccacg
    13081 ccgccgctgc ggatctgctg gccgaactcg tcccggtagt tggtgaccag actcacgccc
    13141 gccaccatca cgtcgtagac cttccaggca tcgccctgct tgtccagggc gtagtcgatc
    13201 tgcaccggct tggcgcccgg ctgacggact tcggtgcgga ccaccacgtc ggtgtcttcc
    13261 ggcttggcct tgaggggctt gaagtcgatc tgctggttgc gatactgggt cagggcattg
    13321 gagtaggtgc gcaccagcag ggtcttgaac tcgtcggtga gggcgctctt ctggtcggcg
    13381 ctggcgctct tccagtcctt gcccacggcc aggcgggtca tgtgggcgaa gtcaaaatag
    13441 ggcagcacct tggtttcgac cagctcgacg gcgcggcggg tgtcgccgga ctggatcgcc
    13501 ttgtcctggc ggacgatggc gatgacgtcg tcggtgacgc ccttgaccag ggcgtcgggc
    13561 gccaccgcgt tggcaaaggc ggccgcctgg aaacccaggc tgagggtcag ggcggcgagg
    13621 aaggaggcgg tcttcttgat cattcctggt tctctctctg ttcggatggg gcggctgcca
    13681 ggggcgggac ggcagcggcc ggcacatcga gcgccgcgtc gtcgtccggg tccttcaccc
    13741 gcggcgggga gccgtcgtac acctggttct gccggtattg caggtaaaag ctgcgcatgt
    13801 agccatactt gtcgagggcg gcctgctcgg cggccgtgtc ggcgcccagc agctgcgccc
    13861 gcttggcgac caggcgcagg gccgtggtgc ggttgcgctg gcccatgtcg tcgatctgcc
    13921 acaccgggtc catgttcaga tccaccagca gcagactcgt tccgtcccgc agggtgctcg
    13981 gcccgaggaa gggcagcacc agatacgggc cggagccgac gccccaggcg cccagagtct
    14041 ggccgaagtc ctcgtcatgc ttgtcgagcc ccagctcggt ggcgatgtcg aaaacaccga
    14101 gcatgccgac ggtggtgttg atcaggaaac gcccgccgtc cgacagaccg tcgaggacct
    14161 tgccctggag caggttattc acgccgatcc acaggtccgc cacgttgccg aagacgttgc
    14221 tgacgccggt cttgatgggc agcggcgcca ccgtgtcgta ggcttcggcg accggacgca
    14281 tcacggcccg gtcggccatg tcgttgaagc ggaaggtggc ccggttcatg gactcccagg
    14341 gatcctgggg attgccgccg gacgaggcgc atgcacccag accaccggcc agcagcaggg
    14401 tcgtcgcgat gcgggccggg acccgcagga aatttaggct gttcttcttc atcgtcgttc
    14461 cgcagccggt tggggccggg tgcgctctcc gtggccggcg gctacttggc cggcgcctga
    14521 tcgccgtctg ccgccttgtt gaacatgaac tggctgatga gcttttcgag tacaacggcg
    14581 gactgggtct tcttgatgac ttcgcccggc gccagggtct tctcctcgcc accgggatcg
    14641 aggccgatgt actgctcgcc gagcaggccc gaggtgagga tgttggcgaa ggtgtccgtc
    14701 ggaaacttgt agcgtccgtc cacggaaagg accacctccg cctggaagcg ttcattgtcg
    14761 tagcggatct cggcgacgcg gcccaccagc acgccggcgc tcttgaccgg cgcgcgcacc
    14821 ttgaggccgc cgatgttgtc gaagcgggcc gacagggtgt agggcgactg cacgtcgctg
    14881 ccggaaatgc tgcccacctt cagcgccaga aagagcagcg cggcgatgcc catcgccacg
    14941 aaaaccccca cccacagatc gagcgtcgtg cggctcatcg tcaacctcgg aacatgaaag
    15001 acgtcatcac aaaatccagc gccaggatcg ccagcgcgga actcaccacg gtgcgggtga
    15061 tggcccgcga cacgccctcg gcggtgggca cgcaatcgta gccctcgaac acggcgatca
    15121 gcgccaccgc cacgccgaac acggcgctct tgatgacgcc gttgagcacg tcgtagcgga
    15181 aatccaccgc gccctgcatc tgcgaccaga aggcgccgtc atcgacaccg atgaagatca
    15241 ccccgatgag ccagccgccg aagatgccca tcgccgagaa cagggccgcg agcaggggca
    15301 tggagatcac gcccccccag aagcgcggcg ccaccacgcg ggcgatgggg ctcaccgcca
    15361 tcatgtccat ggccttgagc tgctcggtga ccttcatcag gccgatctcc gccgtcaccg
    15421 ccgaaccggc gcggctggca aagagcagcg ccgccaccac cggccccagc tcccgcacca
    15481 gggacagggc caccagggtg ccgagggcgt cggccgagcc gtaacgctgg agcgtctcgt
    15541 agccctgcag gccgagcacc aggccgacga agagaccgga gacgaggatg atcagcaggg
    15601 acagcacgcc gctgaagtag agctcccgca gggtcaggtt caggcgcagg aaggactggc
    15661 cggaatacac caggatcagc cagaagaagc gggcgatgaa gcccagctgc cagagggcgt
    15721 cgatggccgc ggccccgagc cggcgcagga tgtccagcat cagcgcggcc ctccggtgag
    15781 ggccgtggcg taatcgccgg ccggatagtg gaagggcacc ggcccgtcgg cttccgcgtg
    15841 gatgaactga tgcacgaagg ggtcggtcgt gttgcggatc gcctcgggcg tgccttcggc
    15901 caccacccgg ccctccgaga tgaaatagat gtaatccacg atctgcaggg attcctgcac
    15961 gtcgtgggtg acgatgacgg agctcgcccc cagcgcatcg ttgaggcgcc ggatcagctg
    16021 gccgatcacg cccagggaaa tcggatcgag gccggtgaag ggctcgtcgt acatgagcaa
    16081 cgtagggtcg agggcgatcg tgcgggccag cgccacgcgc cgcgccatgc cgccggacag
    16141 ctcgcccggc atcagatcgg ccgccccgcg cagccccacc gcgtggagct tcatcatcac
    16201 cagatcgcgg atcagctcgg gtggcaggtc agtgtgctcg cgcagcggaa aggccacgtt
    16261 gtcgaacacc gtcatgtcgg tgaacagggc cccgaactgg aacagcaggc cgatgcgccg
    16321 gcgcagcgcg taaagcgcct cgcgaccgag ggcgcccacc tcctgaccgt ccacccggac
    16381 cgagccggac tgcgcccgct cctggccgcc gatcaggcgc agcagcgtgg tcttgccaca
    16441 gccgctaccg cccatgatgg ccaccacctg accccgcgga atcgacaggg aaatgtcgcg
    16501 caggatggcg cgttcgccgt aggcgaagcg cacgtgctca agacggacaa ggggctcgga
    16561 catggaaaat gctgcaatgc gggagcgcgg attgtagcag aggcgtgctg ccgtggcatg
    16621 cccgggacaa ccctgacagg ccgaaatacc ccgtcacatc aatgtcttga tgcagcgggc
    16681 gcgcctgtcg cgcgagcagt ctataatgtg cggtttggtt ccacttgccc gcctttccgc
    16741 ccatgcagga caaatacctt cccgccgaca tcgagcgcgc cgcccaacag cattgggaca
    16801 gcacccaggc cttccgcgtc accgccgacg cctcgcgccc caagtattac tgcctgtcca
    16861 tgttcccgta tccgtcgggc aagctgcaca tgggccacgt gcgcaactac accatcggcg
    16921 acgtgctctc ccgctttcac aagatgaagg gctacaacgt cctgcagccc atgggctggg
    16981 acgccttcgg cctgcccgcc gagaacgcgg ccatcaagaa ccgcgtcccg ccggccaagt
    17041 ggacctacga caacatcgac tacatgaagg gccagctcaa gtccctgggc ttcgccattg
    17101 actggagccg cgaactggcc acctgccgcc ccgagtatta caagtggaac cagtggctgt
    17161 tcctgcgcat gctcgaaaaa ggcatcgccg agcgcaagac ccaggtggtg aactgggacc
    17221 cggtggacca gaccgtgctg gccaacgagc aggtcatcga cggcaagggc tggcgcaccg
    17281 gcgcggtggt ggaaaagcgc gagatccccg gctactacct gaagatcacc gactacgccg
    17341 acgagctgct gtccgacctg gacagcctca ccggctggcc cgagcgggtc aagctgatgc
    17401 aggccaactg gatcggcaag agcatcggcg tgcgcttcgc cttcggccac gacatcgccg
    17461 gcgatgacgg caagctcatc agcgacggca agctgtgggt gttcaccacc cgcgccgaca
    17521 ccatcatggg cgtcaccttc tgcgccgtgg ccgccgagca tcccctggcc gtccacgccg
    17581 cccaggacaa ccccgccctc gccgccttca tcgagaagtg caagcacggc tccgtcatgg
    17641 aagccgacat ggccaccatg gagaaggaag gtctgcccac cggattgttc gtcacccacc
    17701 cgctgaccgg cgaacaggtg gaagtctggg tcggcaacta cgtgctgatg agctacggcg
    17761 acggcgccgt gatgggtgtt ccagcccacg acgagcggga tttcgggttt gccaagaaat
    17821 acggcatcgc gatcaagcag gtcatcgcca acggcgacaa ggccttcggc ctggacggct
    17881 ggcaggagtg gtacggcgcc aaggacggcg tctgcgtaaa ctccggcaag tacgacggtc
    17941 tcggctacga agccgccgtc gatgccgtcg ccaccgatct cgccgccctg ggcgtcggcg
    18001 agaagaaagt ccagtggcgt ctgcgtgact ggggcatctc ccgtcagcgc tactggggct
    18061 gcccgattcc catcatccat tgcgatgcgt gcggatccgt ccccgtgccc gacgaccagc
    18121 tccccgtggt gctccccgaa gactgcgtgc ccgacgggtc cggcaacccg cttcacaagc
    18181 gcgacgactt catccacacg acctgtccct gctgcggcaa gccggccagg cgcgaaaccg
    18241 acaccatgga caccttcgtg gattcgtcct ggtactacgc ccgtttcgcc acgggcaacg
    18301 gcgcgaatac aatggtggat gccgagaccg actactggat gaccgtggac cagtacatcg
    18361 gcggcatcga acacgccatc ctgcacctgc tctattcccg cttctggacc aaggtcatgc
    18421 gcgacctggg cctggtgagc taccgcgaac ccttcgccca cctgctcacc cagggcatgg
    18481 tgctcaacca cgccttcttc cacaaacccg atggcggcgg caagaactac tactgggaaa
    18541 gcgagatcga cctgctgcgc gactccaagg gccaggtcat gggcgccaag ctcaagaccg
    18601 acggcacggt gctggagcat gagctcacca ccatgtccaa gtccaagaac aacggcgtgg
    18661 atccgcaggc cctcatcgag cagtacggcg ccgacaccgc ccgcttcttc atgatgttcg
    18721 ccgcgccgcc cgagcagacc ctcgaatggt ccgacgctgg cgtcgaaggc gcctaccgct
    18781 tcctcaagcg ggtgtggagc ttcggacacg tcttccacag cgagatccgg ccgcagctgg
    18841 gtgcagaacg ccagctcgcc acggtcaagc tgccggacga cctggccgcc ctgcgccgcg
    18901 agctccatct gctcctcaag caggccaact acgacctggg caagcagcag ttcaacaccg
    18961 tggcctccgc caccatgaag atgctcaatg ccctcgagaa ggccccgcgc ggcggccccc
    19021 tcgccgccga ggtcatcgaa gaaggcctgg ccatcctcct gcgcgtgctg tcaccgctca
    19081 cgccccacat cagccacagc ctgtggcacc agctgggtta cggcgacgac atcctcgccg
    19141 ccgcctggcc tgagcccctt gacgccgccc tcgcccagga cgcggtggac ctgatgctgc
    19201 aggtgaacgg caagctgcgc ggcgccgtgc gcgtggccgc cgatgccccc aaggatgtca
    19261 tcgaagccgc cgccctggcc cacgaggcca ccgtcaagtt cctcgaaggc cgcgccccga
    19321 agaaggtcgt ggtcgtcccc ggccgcctcg tgaacgtggt tggctgagcc cccggagaaa
    19381 gccatgcgcc cagtcgcctg cctgatcgtc agcctgctcg ccaccaccat cctgggcgcg
    19441 tgctccaccc agggtctcta cgagggtatc cggcaggaga acgtacgacg ccagaatgcg
    19501 ccgggccgcg atgtcagccc cgacccggcg gcgccgtcct acgatgccta caacaaggaa
    19561 cgcgagcgcg tcctgaagga atcccgccca tgagcccgct tccccgctcc gccgcccgcc
    19621 ggcgactcct cggcgccctg ctggccgggc cgctgctggc cagcggttgc ggcttccagc
    19681 tgcgcggtcc gcgcgccctg cccttcgaat cgatctacct gggcatgagc gaatattccg
    19741 aactggccgc agccgtccgc cgccagatcc gcgcgggcgg cccgacccgc atcgccgacc
    19801 gccccgacga cgcccaggtc cgcctggaag tcctgtccga cgcccgcgaa aaggtcatcc
    19861 tggccctcaa cacccagggc cgggtgcgcg aataccagct ccgccagcgc ttcgccttcc
    19921 ggctcgtgac caaggacggg cgggaggtga tcgcccccaa cgagatcacc ctgcgccgcg
    19981 acctggcctt cgacgactcc cagctcctcg ccaaggagca ggaagagatc ctgctctacc
    20041 gggacatgca gaacgatctg gtgcagcagc tcatgcgccg cctcgccgcg gccaagatga
    20101 cgccggccca gccgtgatcc tgcgccccga gcagctcgcc gcccagctcg acaagcccct
    20161 cgcgcccctt tacgtcctgc acggtaacga gcccctgctg gtgctcgaag ccggcgacgc
    20221 cctccgcgcc gccgcccgcc gacagggcta ctccgagcgg gaggtgctcg tcaccgggca
    20281 gggcttccgc tgggacagcc tgcagtcggc cgccggcaac ctgtccctgt tcggtgccag
    20341 caagctggtg gatctgcgca tccccaacgg caagcccggc cgcgacggcg gcgaagccct
    20401 gcaacgctac gcccgcagcc tcgacgccgg cgtcgtgacc ctcgtcaccc tgccggagct
    20461 ggactgggcc gcccgcaagg ccgcctggtt cacggccctc gccgaagccg gcgtggtcgt
    20521 cgaactcaac gccccggagc gggaacgcct gcccgagtgg atcgcccagc gcctcgcccg
    20581 ccaacagcag aaggcgcagc ccgaggccct cgccttcatc gctgatcacg tggaaggcaa
    20641 tctcctcgcc gcccaccagg aaatcctcaa gctgggcctg ctccacccgc cgggcgaact
    20701 gggcctcgac gccatccgcg acgcggtgct caacgtcgcc cgctacaacg tggacgccct
    20761 gcgcgccgcc ctgctggatg gcgacgccgc ccgctgcgcg cgcctgctcg aaggcctgcg
    20821 cggcgaaggc gaggcgcctc ccctggtgct gtgggccctg gccagcgaag tccgcaccct
    20881 cgccggcctg cgccagggcg tcgattccgg ccagccgctg gcggccctcc tcaaggccga
    20941 acgggtcttc gacgagcgcc ggcgccaggg cctgcagcgc gcccttccgc gcctgggcgg
    21001 cggcgcgctg aacgccgcgc tcttgcacgc ggcgaggatt gaccggatga tcaagggtct
    21061 ggccggcggc gacctctggg acgaatgcct gcaactgtgc ctgcgcctgt gccgcagccc
    21121 cgccccggcc cgcaccgcga actgaacgag acacgatgga catcaagacc tacatgcagg
    21181 gcgtcggccg tcaggcccgc gccgcctcac gccaggtggc cgccgcctcc accgacgcca
    21241 agaacaccgc cctcctcgcc atggctgccg agatccgcgc ccgccgggcc gaactcgccg
    21301 ccgccaaccg ccgcgacctg gaagaagccc gcgccgccgg cctggagccg gccatgatcg
    21361 accgcctgac cctcaccgaa aaaggcatcg acggcatggc cgccggcctc gaacaggtcg
    21421 cagccctgcc cgacccggtg ggcgagatga acgagctcaa gcgccgcccg tccggcatca
    21481 ccgtcggcaa gatgcgcgtg ccgctgggcg tcatcggcat catctacgaa gcccgcccca
    21541 acgtgaccgc cgacgccgcc gccctgtgcc tcaagtccgg caacgccgcc atcctgcgcg
    21601 gcggcaagga agccttgcac tccaaccagg ccatcgccgc ctgcgtgcgc gccgggctcg
    21661 tcgccgccag cctgcccgaa gccgccatcc aggttgtcga gaccactgac cgggccgccg
    21721 tgggcgaact catcaccatg cccgaattcg tggacgtgat cgtcccccgt ggtggcaagg
    21781 gcctcatcga gcgcatcacc cgcgaggccc gcgtccccgt catcaagcac ctggacggca
    21841 actgccacgt ctatgtggac gcctttgccg accccgccaa ggccctggcc atcgtcgaga
    21901 attccaagac ccagcgctac ggcacctgca acaccaccga atccctggtg gtggatgaga
    21961 gcgtcgccgc cacccagctg cccgccctcg ggcgcatgct cgccgccaag ggcgtggagc
    22021 tgcgcgcctg cccggtggcc ctgcccatcc tgcgggacgc cggcattccc gatgtgcagc
    22081 tcaaggccgc ggtcgagtcg gactggtacg aggaattcct cgcccccatc atcgccgtca
    22141 aggtggtgga cggcctggat gcggccatcg acttcatcaa cacctgctcg tcccagcaca
    22201 ccgagtccat cgtcaccgaa aaccacacgc gggccatgcg cttcctgcgg gaggtggatt
    22261 ccgcgtcggt gatgatcaac gcgtcgacgc gcttcgcgga cggcttcgaa tacggcctgg
    22321 gtgccgagat cggcatctcc accgacaaga tccacgcccg cggcccggtg ggcctggaag
    22381 gcctcaccag ccagaagtgg atcgtcttcg gcgacggcca cgtgcgcgcc tgagcgtcgt
    22441 catcgccctt ccctgccggg ggcttctcgg ctaggattca aacgcccgtt cgaatcgagc
    22501 ctccgcccga tccggcgggc gttttcccat accaagaatc caggagacag cccatgccca
    22561 ccccgttccg ccacgccctg gccgcgctgc tgctgagcac cgcgctcggc acgccctcca
    22621 cctttgccgc cgacgtcgcc ggcgtccatt tcgacgacca cgccgccgtc gccggcagcg
    22681 acaccgtcct caatggcgcc ggcctgcgta cccgattcat gctcaaggtg tacgccatcg
    22741 gcctgtacgg gcccaaacgc gccgacacgg tcgagacctt cgccgctgtg ccgggcccca
    22801 aacgcattca gatcgtgacc ctgcgggagc tcaccgccga acaattcgcc gacgccctcc
    22861 tggacggaat aagaaaaaat accagcgacg cggaattcac cagactccag ccccgtgccg
    22921 acgaattccg cgcccaattg ctggcgctca aatccacccc gtccggcagc cagatccgca
    22981 ttgaatggct gcctgccagc ggcacccgcc tcagcgtcgg caatgaggtc cgcggccgcg
    23041 acattcccgg ggaggatttc ttccgcgccc tgctacgcat ctggctcggc gaaaaaccgg
    23101 tcgatggcga attgcgcaac gccctgctcg gcaaagcctg aacgcaatca caccggacga
    23161 tggaaccgat cttcagcgcc atcgtaccgg ccccgttcgg ggcggtgggc attgcaactg
    23221 cggggggctg cgtcaccgaa atcgtcttcc tgccacccgg cacggcgggc ctcgcgcccg
    23281 ccgatcccct ggcagatcgg gcttttcggc agatcgaacg ctatttcgcc gatccggata
    23341 ccgccttttc ccttccatta cggcctcaag gcagtccatt ccgccagcgt gtctggcagg
    23401 ccattgccac cattccccgc ggagaaaccc gctgttatgg cgaattggcc cgggctctgg
    23461 gcagcgcggc ccgcgccgtc ggtcaggcct gcggcgacaa cccctttccg ctggtcattc
    23521 cctgtcaccg cgtgatcgcg gccgcaggtc ccggcgggtt tgcccatcac cgcggaggct
    23581 ttctcctcga caccaaacgc tggctgctcg cacatgaagc gcctgggcct tttgcggaat
    23641 gcagggattg accgcggcct gccgtagcgc cggcgccgaa gtggacgcac aatagtcccg
    23701 gcgcccccgt gcgcacggaa tcgcgtacat atggatcact tcgcgctccg tcccgcgcca
    23761 tcctgtccgc ccagcccaac tgacgcattc cttttccagc ctgtattcac catcatgtcc
    23821 cgtcacgacc cgcaccctcc ggagacgcct gccacccgtt tcctgcgcca gcagcacatt
    23881 gcccactcca gccacctcta tgcctacgag gatcatggcg gcactgccgt ttccgcgcgc
    23941 gagctgaatg tcccggagca cgcggtgatc aagactctcg tcatggagaa cgaaaggcgg
    24001 gaggccctca tcgtgctcat gcatggggac cggaaagtct cgaccaagga gcttgcccgc
    24061 cagaccgggc ataagcagat cgccccctgc gacccggccg tggcccagcg ccataccggc
    24121 tacatggtgg gcggcacctc ccctttcggt acccgcaagg ccctgccggt attcatggag
    24181 cagaccatcc tcgaactccc gctcatttat atcaatggcg gcaaacgggg tttcctggtc
    24241 ggcattcacc cccacgatat catccggacg ctccacgcaa agctggtgca cgttgccatc
    24301 gaatgaacat cggcctcacg ctattcaatt ggcaacggtt gatgcacaat tcgcccatac
    24361 tcaaaattga tcatccggcc aatcccgcgc cagcgccgcg cccccggtgc acccgacttt
    24421 ctgacaagga atttcaccat ggacatttcc ggactctcgc tcaccgaatt gcgccgtctg
    24481 cagacccgca tcgaagccga aatccgccga cgttctgaca ccgcccgaaa ggaactcctc
    24541 aagcgcatgc agaaactggc tgccgagcac ggcatgagtc tcgccgacgt cctcgggcag
    24601 gagtccgaaa agcccgccgc gcctgccagg aagacgcctc ccaaggcgct tgcacagccc
    24661 gcccgcaagg cggccaaacc ggcctcggtg atcaagttcc gccatcccga aaacaccgcc
    24721 atcggctgga ccgggcacgg ccgcaagccc cagtgggtca tcgactggct ggcccagggc
    24781 aaggcactcg aagacctgcg cgcctccacc gaagccgcgg cctgagcgct ttcgtcacgc
    24841 cttatgcgtc aatgcaacga aaaaaggagt gccggtccgg gcactccttt ttcttgcggc
    24901 agttctattc acccgggcaa acccgcggtc ggtgcatgtt ccggtggcgc acttccgtta
    24961 cggccccgct gaatgaatac acccacccgt ttcacacctt tcatcacgcc cattttggca
    25021 attgccaatt tcacccaggg cgcaccaaat tcatcaaaaa gcaatttgac gacgaattca
    25081 cccagggttt ctatcagatt gaactgacgc acggccagct ccgcccgcaa tcgctcgatg
    25141 acctgggcgt aatcgatggt gtcggcgata tcatcgtgct cggcggccgc atcgggcact
    25201 ccgaaggtca gattgagctc gagggtctgc tccgcgaccc gctcgcgggg atagatgccc
    25261 acccgcgcct gcacccgcag tgcttcgata aaaatgaaat ccatcgcatg tcccggctaa
    25321 aatcgccgcc attctagccc gcccaccgga accgcatgca aaccctgctc ctcctctgcg
    25381 ccgcctacct gatcggctcc gtcccctttg cggtagtggt cagccgcgcc ttcggcatgg
    25441 ccgacccccg cacatacggt tcgggcaatc ccggcgccac caacgtcctg cgcagcggta
    25501 acaagctcgc agccgccctc accctgctcg gcgacgcgtt caagggttgg ctggcggtgt
    25561 tcctggccca cctgctcgtg cccacgcttg gactcggacc cgaagtggtt ccggcctgcg
    25621 cactggccgc ctttctgggg caccttttcc cagtatttct gggctttcgg ggcggtaaag
    25681 gcgtggccac tgccgccggc gtgttgatcg gtgcgtccgg ctggctgggt ctggcgacgc
    25741 tcggcgtttg gctgggcgtc gccttcggcg cccgctattc gtcgctggcc gccctctgcg
    25801 ccgcgctggc ggctccgggc ctgggcttcc tgctcggtct cgatccctac tggattctgg
    25861 ctatcagcgt catgtcgggg ctgctgatct ggcgccacag cgacaacatc cggcgtctgc
    25921 tggcaggcga ggaaagccgc atcggcagca agaagaaggc atagcgcacc gcgccgccga
    25981 accggcgacg cctctcacac cggggtgccg atttcctgca ggctccagcg cgggcgcacc
    26041 gcaaacgcgc cgtccccggt ccgccccggc gcaccggcgg caaagcgcag cgcgccggcg
    26101 aaggcgatca tcgcgccgtt gtcggtgcac agcgccagct cagggtagaa caccgtcgcc
    26161 ttgcgccgcg ccgccgcccc atcgagctgg tcacgcaagc ggcgattggc accgacgccg
    26221 cccgccacca ccagctggcg cagccccgtg tgcctgaggg ccgcgaggga cttggccacc
    26281 agcacgtcca ccaccgcctc ctggaactcc gccgccagat ctgccttgtc cgcgtcggtg
    26341 aagtccggtg ccgacgccgc attgagcacg gccgtcttga gcccgctgaa actgaagtcg
    26401 aagtccttgc tgtggagcat cggccgcggc aggcggaaac gtccgggccg ccccgcctgg
    26461 gccagcgtcg ccagcgccgg gccgccggga tagcccaggc cgagcagctt ggcggtcttg
    26521 tcgaaggcct cgccggccgc gtcgtccacg gtctcgccga ggaggtggta atcgcccacg
    26581 ccggtgacgc gcatcagctg ggtgtggcca cccgacacca gcagggccac gaaggggaag
    26641 gccggtggcg tcgccgacag gagcggcgac agcagatgcc cttccaggtg atgcaccggc
    26701 agcaccggca ggtcgagcgc cagcgccagg gattcggcaa atgccgagcc caccagcagc
    26761 gcaccggcaa ggcccggacc ggcggtgtag gccaccgcat ccacgtcggc cagcgtcgtg
    26821 cctgtctttg ccagcagttc cctcagcagc agcggcaggc ggcggatgtg atcgcgggac
    26881 gccagctccg gcaccacgcc gccatacacc gcgtgcaggt cgatctggga atgcaggcag
    26941 tgcccgagca ggccccggcc cgtctcgtag agggcgacgc cggtctcgtc acaggaggtt
    27001 tcgatgccca gaactcgcat ggtcccgtcc gtgcaaaagc cggtatttta gcgcggccgg
    27061 tcacgcgcgt cgaaaaaagg gcttggttgt caagcgggcc ttggttatac tccatggttt
    27121 tcccgctcac agaatccaga ttttcaagga agcacgcaat gccgggtatc cgcgtcaaag
    27181 aaaacgaacc gttcgaagtt gccatccgcc gcttcaagcg caccatcgaa aaagtgggtc
    27241 tgctgcctga actccgcgcc cgcgagtttt acgaaaagcc cactgccgaa cgcaagcgca
    27301 agctcgccgc cgccgtcaag cgtcaccaca agcgcctgcg cagccagacc ctgccgccca
    27361 agatgtactg agcggcacct gtacgggctt cccgatcgcc acgcgctgac gcacctgtgt
    27421 gcgtagcgac gtggcgttcg tgctttgcgg catgcccgtc gcaaagttca gcgcgagcac
    27481 tccaacacta cgccgcaaca tcaccgcgcc atcgactgtc gatggcgctg ccgggtgacg
    27541 tcgagcgctg cccaggcctg tccgtcgtcc gttccgtcgc gacctcaccg ctcgtccggt
    27601 gacggcgcct ttgcccaccc tcgccctgga gcccgaacat gtccctcaag gatcgcatca
    27661 acgaagacat gaagaacgcc atgcgcgcca aggacgctcc gcgcctgtcc gcgctgcgcc
    27721 tgctgctggc cgccatcaag cagcgccagg tggatgagcg gatcgagctg gacgacgacg
    27781 gcgtgatcgc cgtggtggac aagatgctca agcagcgccg cgactccgtc gcccagtacg
    27841 aggccgccca gcgctttgac ctggccagtg ccgagcgctt cgagatcgac gtcctcaccg
    27901 cctacaagcc ggccggcctg ggcgcggacg agatcgccgc ggccatcgag gccgccgttg
    27961 ccgccaccgg tgcggccggc cccgccgaca tgggcaagct gatggccgtg ctcaagccgc
    28021 agctcgccgg caaggccgac atggccgagg tgtcgaaact ggtgaaaacc cgactcgcga
    28081 actaggattc atgggaacgg cgccgcctgc gccggccgtt gccacatgat cccgcacgcc
    28141 tttgtccagg acctgctcgc gcgggtcgac atcgtcgaag tcatcgagcg ctacctgccc
    28201 ctcaagaagg gcggcgtcaa ctacttcgcc tgctgcccct tccacgggga aaaatcccct
    28261 tccttctccg tcagcccgtc caagcagttc taccactgct tcggctgcgg cgcccacggc
    28321 agtgccatcg gcttcctgat ggaatacagc ggcctcggct tcgttgaggc tgtcaaggac
    28381 ctggccgagc aggtcggcat gcaggtgccc gaagaagccc ccggcgccgc ccaggaacgc
    28441 gtccgcgaac agtccctcac cgagctgatg acccaggccg cgcgcttcta caaggagcag
    28501 ctcaagcgca gcccggtcgc catcgaatac ctcaagggcc gcggcctcac cggcgagatc
    28561 gccgcccgct acggcctggg ctacgcgccc gagggctggc agagtctcca ggcggccttc
    28621 gccgactacg ccgaccccaa gctgctcgaa tgcggactcg tcatcgagaa cgaccagggc
    28681 cgccgctacg accgcttccg agaacgcatc atgttcccca tcctcgacca gcgcggccac
    28741 gtcatcggct tcggcggccg ggtcctgggc aagggtgagc ccaagtacct caactcgccc
    28801 gaaacaccgc tgttcgagaa agggcgcgag ctctacggcc tcgtccaggc ccgcggcacc
    28861 atccgtgaca gcggcaccgt gctggtcgtc gaaggctaca tggacgtggt cgccctcgcc
    28921 cagttcggcg tcgccaacgc ggttgccacc ctgggcaccg ccgccacgcc ccaccacatg
    28981 cagaagctcc tgcgcgtcgc cgaccgggtg gtgttctcct tcgacggtga cgccgccggc
    29041 cgccgcgccg ccggccgggc gctggaggtg tgcctcgaac acctggccga caacaagacc
    29101 gtcgccttcc tgttcctgcc ggacgatcac gacccggaca gctacgtgcg cgccttcggg
    29161 gccgacgcct tccgccaggt cgtggaccac gccaccccgc tggccgattt cctcctcgcc
    29221 caactgcgcc aggacatcga cctcgccacc gccgaggggc gcgcccgcct ggtccatgaa
    29281 gccaagcccc tcgtcacccg catcggcgcg ccgctgctgc gcctgcaggt gatcaagatg
    29341 ctcgccgaag cggctggttt cagccagaac gaagtggagc aggcctgtgg cctcgccgcg
    29401 ccacgccgca gcgaacggcg cttcgacgac accccgccgg acgacggctt cgctgctcgc
    29461 cgcttttccg gccaggcgtc gtccggcaag cccttccgcg gcgaacgccg gcccgctgtc
    29521 ccgcgtcagc caccccgcca gcgccctgcc acgcccgtcg agaccctgct caagatcgtc
    29581 gtccagcacc ccacatggag cgcgcgcctg cccgttgacc tgctgcccgc cgacagcgcc
    29641 gagggcctcg ccctgatcgc cgtcaccgac gccatgagcg tcggcgacct ccccacccag
    29701 ggcgtaggcg ccctgctgga gcacttccgc gacacccccc acgcgccgat cctggcccgc
    29761 gtcgcggtcg ccaatgagga aaccccgttc gaggacggga cgattgaacc cctgctcaac
    29821 gacacactca acaagctgca ggccgacgcc ctgagccgcg aaatcgagcg cctgaccgcc
    29881 caggaccggg ccggcggcct cgacccgcag cagcgccgcc gcctcgccga gctgctgatg
    29941 gacaaacaga ggctacgcgt tcgccccaaa gtgacggatt cgtaatataa ttttcggttt
    30001 cgcgaaaatc gcctgccgag tacccgagga cagtcatgac cactgaaaaa ccgaagaccc
    30061 cgcgcaagac gccggccaag ccgcgagccg ccaaggtcaa ggacaaactc gccccgatga
    30121 tcgccgaggc gccttccgct gaagaggcgg aagtccgtcg ctcgcgcctc aagagcctga
    30181 tcgccctcgg caaggaacgt ggctacctca cctacgccga aatcaacgac cacctgcccg
    30241 acgaccttgt cgatgccgag cagatcgagt ccatcatcag caccttcaac gacatgggca
    30301 tccaggtctt cgacgtggcc cccgccgccg aagacctcct catgtccgaa gtcgccccca
    30361 ccgtggtgga cgacgagcag gccgaagccg aagccgagca gaccctctcc gcggtcgatt
    30421 ccgaatttgg ccgcaccacc gaccccgtgc gcatgtacat gcgcgagatg ggcaccgtgg
    30481 agctcctcac ccgcgaaggc gaaatcgaga ttgccaagcg catcgaggaa ggcctcaagc
    30541 acatgatcca ggcgatctcc gcctgtccca ccaccatcgc cgacatcctc gaatccgccg
    30601 accgcatcag caaggatgag atgcgcatcg acgagctcat cgacggcctc atcgacccca
    30661 acgcggttga ggacatcgaa gccatgtccg acagcgacga gctggacgtg gacgaagacg
    30721 ccgaggacga ggacgaagac ggcgacgacg gtgccggcgg tgccgccgtg tccgcctccc
    30781 tcatcaagct caaggccgac gccctggaac gcttcgacgt gctgcgcggc atctacacca
    30841 agatgatcgc cgccctccag aagaagggcc cccaggacaa gacctacgtc aagctccagg
    30901 agcagatctc caccgagctg atgaacatcc gcttcactgc gcgcaccacc gagcgcctgt
    30961 gcgacgccgt gcgccacatg gtcgagcagg cccgcagcca cgagcgcaag atcctgcacc
    31021 tctgcgtgga caaggcccac atgccgcgca cccacttcat caagagcttc cccggcaacg
    31081 agaccaacct ggactggctc aaggtcgaga tcggcaccaa cccgtccaac gccgccgtgc
    31141 tcatgcgcat gctgcccgcc gtcgtcgagg agcagcaaaa gctcgtcgaa ctgcagaccc
    31201 gcatcggcat cccgctcaag gacctcaagg acatcaacaa gcagatgtcc accggcgaag
    31261 ccaaggcccg ccgcgccaag cgcgaaatga ccgaagccaa cctgcgtctg gtcatctcca
    31321 tcgccaagaa gtacaccaac cgcggcctgc agttcctcga cctgatccag gaaggcaaca
    31381 tcggcctgat gaaggcggtg gacaagttcg aataccgtcg cggctacaag ttctccacct
    31441 acgccacctg gtggatccgt caggccatca cccgctccat cgccgaccag gcgcgcacca
    31501 tccgcatccc ggtgcacatg atcgagacca tcaacaagat gaatcgcatc agccgccaga
    31561 tcctgcagga gaccgggcag gagccggacc cggccaccct cgccgccaag atggagatgc
    31621 ccgaggagaa gatccgcaag atcctcaaga tctccaagga gcccatctcc atggaaaccc
    31681 ccatcggcga cgacgacgac tcccatctgg gcgacttcat cgaggacaac gccaccatgg
    31741 ccccggccga cgccgcgctg tactccagcc tgcgcgacgc caccaaggaa gtcctcgact
    31801 ccctcacccc gcgggaagcc aaggtactgc gcatgcgctt cggcatcgag atgaacaccg
    31861 accacaccct ggaggaagtc ggcaagcagt tcgacgtcac ccgcgagcgc atccgccaga
    31921 tcgaagccaa ggccctgcgc aagctgcgcc accccagccg ctccgagcgc ctgcgcagct
    31981 tcctggacaa cgaagcctga tcccgtccgg ccggtgcggc cacgcaccca ccggccgccg
    32041 gtgcaggtcg gcatagtctg gtagcatgcc gagcttcacc ggaccgccgc gaagcctgcg
    32101 gtccccctcc ccttttccgg gcccatagct cagtcggtta gagcaggcga ctcataatcg
    32161 cttggtcgcg ggttcaagtc ctgctgggcc caccagcatc ctcacgcaca cggccagcct
    32221 caccgaggct ggccgtgtgc tttccggtgg gcccgccctc acgcccgccg gaaggtcagg
    32281 ttgatccggc acgcgccggt cagcggatgc tcgcccggct tcaccggcgc cacgccatga
    32341 aaggccaggc gcgacgggcc gccccagacg acggcgtcgc cgtggaacag ggaaatcctc
    32401 cgcgcggggt cgcctcggcg cagccccccg aagaggaaga tcgccggcag accgaaggag
    32461 aaggagacga tcggccaggt gaagtccgcc tcatcccggt cctggtgcag ccccatgcgg
    32521 gcacccggcg cgtaacggtt gatgaggcag gcatcgggcg tgaagccgcc aaagccggcc
    32581 gcggctgcgg cccgctcggc gagggtgcgc cacagccgcg gcatggtggg ccacgctgcg
    32641 ccgccgaggg gatcggcagg cgcatagcgg tagccggcgg cgtcactgac ccagccccgc
    32701 gcgccgcagt tgctggtggc cacggacatc gaccggccgc cgggcgtctg catcgtacgc
    32761 aggggcgcgt gggcaaggac gcggtccagt tcggcgagca ccgcggcggc ctcggcgcgg
    32821 acaaatccgc gcaggagcac cgcgccgggg ctcagcgcct ccgggccgac gtcctccggc
    32881 gtccagcctt cgaagagatc aggcgtcgtc acggccgccg cgctccgcct cccgcgtcag
    32941 cagggccgcc ttccgttcca cgccccagcg atagccggac aggctgccgt cgctgcgcac
    33001 cacccgatgg cagggcaccg ccagcgccac cggattggcc gcgcaggcgc ccgccacggc
    33061 tcgcaccgca cggggtgcgc cgacgcggcg ggccagctcg gcgtagctga tcgtgacgcc
    33121 cggcgggatg ccctgcaggg cgtgccagac gcgcagctgg aaggccgtgc cgcgcaggtc
    33181 cagcggcagc tccagcccat cccgcggcgc ctgcaggtag ccggcgactc ggctcaccca
    33241 ggtggcgaag gccgggtcgt cacccaccag gcaggcgcgg ggaaagcggt cctgcagggc
    33301 gcgcaccagc gcatccgggt cgtcaccgag gctcagggcg cagatgccgc gcgggctggc
    33361 ggccaccagc aacgccccca gctcgcaggc ccccacagcg aaacggatgt cctcaccggc
    33421 accgcccgcg cgccaggcgg cgggcgccat gccgaggacc tgggcggagc gggcatagaa
    33481 gcggccggtg gagccgaagc cagcgtccag caccgcatgg gtgaccggtg cccccgccgc
    33541 gagccctgcg cgcacacgac cggcgcgcag ggccgcggcg taggcgcgcg ggctgaggcc
    33601 ggtggcggcc ttgaacacgc gctgcagatg gccggcgctg aggccggcga gcacggacaa
    33661 tgcctgcaag ctgggcggcg tctcggcggc ttggatggcg cggcacaccg tggcgatgag
    33721 ggcgacatgg gggtcggcca gggtgtccgg atggcagcgc agacagggtc ggaagcccgc
    33781 ctcacgggcg gccgccgcgg agtcgaagaa gcgcacatgc tccggtcgcg ggcgccgcga
    33841 cgggcaggac ggccgacaga acacgccggt ggtggtgacg gcgtaccaga aatgcccgtc
    33901 ggccgtgcca tcccgcgcct ggacggcggc ccagcgcggg tcggcggcag tgggtggcga
    33961 ggacgtggag gcagtcatgg ggtgctccgc aaggcccgag cgtgggcgcg atgcctgcac
    34021 tgtacggggc cggacggccc tgcgcatccc gtttcttgcg gtcgaatccg gtggcggcgg
    34081 gctgcgcgtg cagcccctgc acaccgaccc gacctgcgcg ccgctcgcag cggacccacc
    34141 gcccggatca cgccgggctc cgcacccggc ggaatcagcc gagtgccgcc agcgccgcag
    34201 cgtagtccgg ctcgttcttg atctcgggca ccagctccac gtggcgcacc acgtcctgct
    34261 cgtccaccac caccaccgcg cgggctgcca gcccggccag cgggccgctg gcgagggcga
    34321 cgccgtaggc ttcgaggaat tcccgcccgc gcagggtgga cagggtcacc acgttggaga
    34381 ggccttcggc gccgcagaag cggttctggg cgaagggcag gtcggcggag atgcacagca
    34441 ctgcggtgtt gggcaggctg gaggcggact ggttgaactt gcgcaccgag gtggcgcagg
    34501 tgggcgtgtc gatgctcggg aagatgttga gcaccttgcg ctgaccggca aagttcgcca
    34561 gcgtgacctc ggacaggtcc ttggcgacca gggaaaaagc gggcgccgtg gcgccgacgc
    34621 ggggcagatc gccggcgacg gtgacgggat tgccgccgag ggtgacggta ctggacatgg
    34681 gggactcctc tctcatcgat gttcggaaca ggggcacggg ccccggggcg cccatggtag
    34741 gcgaggcgtc cggcgccgcc aacccgcggc acagcagggt ctttgcatcg cccatgcccc
    34801 gcaccgcgcg gctcacgccg gcaggccctg ctcccagtaa ggtaccgggc cgatacgtgc
    34861 ggccaggtag tccgccagca ggcgcacccg gctgggcacg tagcgatggc tggggaacac
    34921 cgcgtagatg cccagttcag ggccgggaaa gtccgccagc agcacttcca gcagtccggc
    34981 ctgcacggcc gccgccgcga tgaagctggg ctgcaggcag atgcccagcc cgcgccgggc
    35041 ggcgtccagc agggcgtcgc cattgttgct ctgcaggcgg ccgcgcaccg gcacccattg
    35101 cagcgtgccc gccacgcggt agggccagct ggcccgcgcg gtaccggtgt agcccaggca
    35161 gggatggtcc agcagatcgt cgggacgcac cggacggccg tggcgggcca ggtactcggg
    35221 cgcggccagg gtgagcatgc gcgacaccgt gagcctgcgc gccaccacgc tggggtcgag
    35281 ctgggtcgtg atgcggatcg ccatgtccac gcccgcctcc accagattca cctggcgatc
    35341 gttgaagtcg atgtccaggc tcacgtccgg ccaggtggtg acgaaatccg cgaccagcgg
    35401 cgccagatga tggatgccga agctcaccgg tacgctcagg cggatcggcc cgcggggggc
    35461 gcgttcgttg ccgcccagtt ccccttccgc gacgcccacc agatcgagga tctcgcggca
    35521 gcgctccagg taggcctgtc ccgcggaggt gagccgcagg ctgcgggtgc tgcgggcgat
    35581 gagcttgacg cccagacgcg cctccagcgc cgcgaccagg cgcgtcacca cggagcgggc
    35641 cagatcgagc tggtccgccg ccgctgcaaa actccccgcc tccgccaccc ggacgaagac
    35701 ctccatcgcc tgaagccgat ccatcattgc atatccagca ataaagattt cccgattaaa
    35761 ggctatttct gtctcaattg aaagcctaca gtggcaccca gttcatcaac ccgccacagg
    35821 agtcctccgt gatcctcgtc accggcgcca acggccgtct tggccggcgc atcgtccagc
    35881 acctgatcgc acggcgcccg ctgcgcgacg caccggcgct ggcggtgagc gtgcgccaac
    35941 cggcgctggc agccgatctg gccgcgcgcg gggtggacgt ccgcgccggc gacttcgacc
    36001 agcccgagac cctcgccacc acctttgccg gcgtgcgcac cctggtgctg gtctccaccg
    36061 acggcccctc cgcgcagcgc atcgcccaac atgcggccgc catctcggcc gcccgcaggg
    36121 cgggcgtgca gcgcgtcctg tacaccagct tcctcgacgt gggcaccgat tcgccggcgg
    36181 agttcgcccg cgtgcaccgc caaaccgaag acgccctcgc cgccagcggg ctggatgcga
    36241 cgatcctgcg caacccgctc tacgccgacg atctgcccgc gctggtggtg cccggcccgg
    36301 acggagacgc cttctgcctg gccgccggca acggcgcggt gagcctggtc tcccgggacg
    36361 atctcgcccg cgccctggcc gccgccgccc tcgccccgcg cctggacaag cgcgtgtatg
    36421 aattgaccgg cagcgaggcc gtcgcctacg cggagctggc cgcgcgcatc gcccgggtca
    36481 gcggccggcc cctgcgctac ctgcccgtga cggtcaccga acaggccgcc acgctgcgtg
    36541 ccgccgggct gtcggaaggg ctggccgagg gcgtcgccag cctcttcgcg gccgtcgccg
    36601 agggccgcct ggcccgcgtc acccatgact ttgctgccct ggtgggccac ccgccccgct
    36661 ccctggactg cctgatccgc gagtacttcg cccctgtcac tgccccgagg accgcatcat
    36721 gagcgcccgt caccccctgc tgttcgtctc ccacggcgca cccgacgtgc tgctgcggcc
    36781 cgctgccacc gtggccctgt gggccgatct gggccggcgc ctgccgcgcc cacgggcgat
    36841 cctcgtcgtc tcggcccact gggcggcgcg ccagcctacc gtaagcacgg tctccgtgcc
    36901 cgccacgatc cacgacttcg gcggcttccc ggaaccgctc tacgccatgg aatacccggc
    36961 acccggagcg ccggacgtgg ccgcccgggt gcgcgatgcg ctggtggcgg ccggcctgcc
    37021 ctgtgccgag gtgcccgacc ggggcctgga tcacggcgcg tggattcccc tcatggtcat
    37081 gtacccggcc gcggacatcc ccgtcgtgca gctcgccctg caaccccacg ccgggccgga
    37141 ctggcaccgc cgcatcggca ccgccctgcg cggcctgcgg gacgagggcg tgctgatcct
    37201 cgcctcaggc gccatcaccc acaacttcgg ctggctggac tggtccggcg gtggcgagcc
    37261 cctgccccag gccaaggcct tcgccgactg ggtcggtgcg gccgtcgccc gcaacgatcg
    37321 ccccacgctg ctggaatacc gccgccagag ccccctgggc gccgccgccc atcccagtga
    37381 ggagcacctg ctgcccctgt tcgtgaccct cggtgcagcc gacccggacg aaatcgccct
    37441 ccgtctggaa ccggaattca cctatggcgg tctggccatg gatgcctacc tgtggcaaaa
    37501 ggacagcatc atcccttaca ccccggagcc ctgaccatga acaccgaacg ctattccagc
    37561 cccgccatcg ccgcccactg gctgatcgcg ctgctgatct tcgccgcctt tccgctgggc
    37621 ctgtacatgc acgagctgcc cctctcgccc accaagctca agctctactc gtaccacaag
    37681 tggatcggca tgacggtgct gggcctgttc gtcctgcggg tgctgtggcg cctcacccat
    37741 cgcccgcccg ccccgctgcc ggcaccggcc tggcaacaga cggcggcaga ggtcacccac
    37801 cggctcctct acgtgctgat ggccctggtg cccctcaccg gctggctgat gagctcggcc
    37861 aagggctttc aggtggtgta cctgggcgtg ctgccgattc ccgacctgat cgcaaagaac
    37921 ccggatctcg gcgaaatcct cgaagaggtc cacgaatccc tcaactggct gctcttcggt
    37981 ctggtcggcc tgcacgtggc cgccgccctc aagcaccacc tgatcgacaa ggacgcgacc
    38041 ctgcgccgca tgctgccctt cggccgcgtc tgacccccga acccgacaca cgagagaagc
    38101 catgaagacc ctgcgcccca ccctgaccct cgccctgctg gcgggtctca ccaccggcgc
    38161 cgcccacgcg gcggacttca cccaggtgct gaccgacaag agcagcctcg ccttcacgtc
    38221 caagcagatg ggcgtgccgg tggatggcct gttccgcaag ttcagcgccc agctcgcctt
    38281 cgacccggcc aagcccgcga ccgccagcac cgtgctggag atcgacctgg ccagcatcga
    38341 cgccggctcc aaggatgcca acgaggaagt cgtcggcaag cagtggttca acgtgaaggc
    38401 cttccccacc gcccgcttcg tgtccaccgc cgtcaaggcc gtgggcggcg gccgctacga
    38461 agtgagcggc aagatgacca tcaagggcca gacccacgac gtggtggcgc ccttcacgtt
    38521 caggcaggac ggcgccaacg gcgtcttcga cggcggtttc gtgctcaagc gcatggactt
    38581 cggcatcggt gaaggcccct ggtccgacgt gagcgccgtc gccaacgaga tccagatcaa
    38641 gttccacgtg gtcgccgccc cggcgcccgc aaagaagtaa ggccgttcgc ccccgcgacg
    38701 gcccagaccc caacccaaaa ccctcaacct gtcataacgg agaacacagc atgaaaaagc
    38761 tcaccgccct gatcctggcc gccggcctgt ccaccgccgc cctcgccgcg ccggaaagct
    38821 acaccatcga cggcagccac accttcccgc gcttcgaata cagccacttc ggctactcca
    38881 cccagctgtc ccgcttcgac aagaccaccg gcaccatcgt cctggatcgc gccgccaagt
    38941 ccggcaccgt ggacgtcacc gtcgatgcca cctccgtgga taccggcttc cccctgctga
    39001 accagcacat ccagggcgaa gacttcttcg acaccgccaa gtacccgacc atcacctaca
    39061 agtccaccaa ggtgaacttc gacggtgaca aggtcgcgtc ggtggagggc aacctgaccg
    39121 tcaagggcgt caccaagccc gtcaccctga ccgtcacctc cttccactgc atgcctcacc
    39181 cgatgctgaa gaaggatgcc tgcggcgcca atgccgtcgc caagatcaag cgcagcgagt
    39241 tcaacgccgg caagtacgcc ccctacgtgg gcgacgatgt caccctgacc atcgccgtcg
    39301 aagcctacaa accgtaatcg ggtaagctcg tggctccggg gtgcgccgcg ccccggagat
    39361 tgcacggaag cctccccatg accgaaccgc agatcgccca gtacgcctcc tacaccgtcc
    39421 ccgtcgaggc cgggcgggaa tacacctggt gcgcttgcgg ccgcagcgcg acccagccat
    39481 tctgcgacgg ccgccacgcg ggcagcggca tcgcgcccaa gaccttcacg cccctgcgct
    39541 ccggactggc ctacctctgc ggctgcaagc acagccgccg cgcgccgttc tgcgacggca
    39601 cccacgacac gctctaggcg gtcacctcgg ccagcatcgc gtccacatcc acctcgtcca
    39661 cgcacttgcg ctgcatgaag cgctcggcgt agcggcgata cacgcccgag cgcaggaaca
    39721 gggcaaagag atccggatcg atgtgtcccc ggtcacgcat cccggccatg atgctcaccg
    39781 cctcggacag gcgcttgccg gtcttgtagg ggcgatccac cgcggtcagc gcctcgaaga
    39841 tgtcggcgat ggccatcatc cgcgccacca ggctcatctc cccgcccttc agcccacggg
    39901 ggtagccgga accgtccatc ttctcgtggt ggccgccggc gatctccggc acggcccgca
    39961 gatgccgcgg gaagggcaat tcggagagca tgcggatggt gtagacgatg tgctcgttga
    40021 ttttgtagcg atcctcgtcc gtgagggtgc cccgggtgat ggagagattg gtcagctcgc
    40081 cccggttgta gagcagcgcc ggtaccttga ggcggaagcc ccacgggttg tccgccggca
    40141 gcaggtccat cgcaccccgt tcatagcggt gctcgggctt gtcggccagc agtggctcgg
    40201 tgaccggcag gcgcacgggc cccgcatcgg cgcggcggcg ccgctcttcc ggcgacaggc
    40261 cgaggcgatc gtccagggtg cgcagccagg tgcgccgggc gatctgctgc aggcgtgcca
    40321 catcctccgg tgccatggac tccgccccct cgttgcaggc cgcaacgaag gcgaactccc
    40381 ggtccagcgc cgcgtgggtc tccaccagcc gacccttggc ggcggcctcg tcggcgcctt
    40441 ccgcgcgcgc ctgccagtag gcgatggtcg cgtcacgctt gagcacctcg aagcgcatgc
    40501 ggatctcgtg gatgcggtcg tacagggtct ccagcttggt ggccttgtcc accacgtact
    40561 cgggggtggt gaccttgccg caatcgtgca gccacgcggc gatatgcacc gcctcccagt
    40621 cctcctcgcc cagccggaaa tcgcgaaaag gtccgtccgc gctgttacag gcggcctggg
    40681 caagcatctt ggtgagctcc ggcacgcggg cgcagtgacc gccggtgtag ggactcttcg
    40741 catcaatggc gccggcgatg agctggatga aggcctcgaa cagctccttc tgggcgcgga
    40801 tcagggcccg cgtctcgacc gacatggccg ccgaaccgga cagggcttcg acgaaatgca
    40861 ccaggtcccg gtccggcggc gcatcgaacc acagcaccag cagccccagg cgggcctgct
    40921 tgcgatccgt caggggaacg gccaggtagg ccagatcctc ggcgccgagc agtccgaaca
    40981 atgtctggct agcggcgccg gcagccgtct gcagggtcag gcgtcccacg ccggagcggg
    41041 cccccgccgg caccagaccc gccatcgtcg cccccgccgg agcctgctcc agcggatgac
    41101 tggcaccgtc cgcgtcccgc aggacgaccg catcgagcgc ctcgccgccg tgggcggcca
    41161 ggtagagcac accggcgcgg gcccccacca cggccacggt ctcatccacc aggcgcggca
    41221 gcaggcgctc gaaactggat tcctcggcca tggccgaggc gatgtcgagg aagcgccgga
    41281 tcgccccctt catggaaccc agatcggtgg ccagattgtc cacctcgcgc accaccgaac
    41341 gggtccgcac cgggctggag aaatcgaaac gccggatcgc cgccgtctcg ccggccaggt
    41401 cgcggatcgg gcgcgacacc aggcgcgcgg ccaggtaggt gaagggtagc gccagcagca
    41461 tcaccagcag ggtgacgctg atggattgat tgcggatgcc gcgcgcctcc acgagcagct
    41521 cctcatccgg aatcaccacg cccaggtaca ccggctgtcc gcccagccat tgcacctggg
    41581 cgacggcgcc gtgccagtcc cgcccggccg cgcggaagct gcccaggggc tcggtctcgc
    41641 cgtcttgggg cagtcgctgg gccagggcct tgagcacgcc gccgcccagc tccgtcagat
    41701 ccggccggcg cgcttcgccc gcctgtggag gcggaagcca gtccttgccc gtataggcca
    41761 gcacgcccag gtcgcggtcg aagagcacca gctgcgatcc cggtgtgatg cgctggcggc
    41821 gcagcgactc gtcgagggtt tccaggcgga tgtcggcccc caccaccgag cggcccgtgt
    41881 ccgtgtgacg ggcgacggtg acgcccagct tgcgggtgga ggcgaacagg taaggctcgg
    41941 tctggatcgg ctcggggccg gccagggcct ggccgtacca gctccgccgg gtggggtcat
    42001 aggcggtgcc gtaatcgggg cgcgggtcac tacgcagggt ggcaagatcc tcatcgagga
    42061 acacgaagcg gccgctgccg tggtcgatgc tctgcagcag gtaggcggcc cccggcggcg
    42121 ctcccatcaa gtcccggtcg gagcggttgg ccaggcgcag gagcaggaag aagtcccggt
    42181 cctcgtagcc gatgtagaag gccgaagcgg acggcgaggc gagcagggcc tcgcggaaac
    42241 tcgccagctg ctcgcggcgc gtctccaggg tgcccgcccg agtcaccggc gagcgagcca
    42301 gcaggcgcac cgaggccccc accggcttga gcagggtttc cgtctcggcg gcgatctgcc
    42361 gcgagatgcg ccccagcaga tcggcggcgg cccgctccat catgtcggca ctgcgggtgt
    42421 aggacaccca cgcgatgctg ccgccggcca tcagcaccag caggaagaac agcgtggaaa
    42481 tgtggatgtg cagcggcagg caccatcgtg cgccgcgccc cctggccccc ttgtccggca
    42541 tcgtctcctc cttcagtgtc ccgccagggc acggcggtat tcgcccggcc gggcgccggt
    42601 ccattttttg aacgcccggt gaaaggcgct tgcctccgcg aagcccagtt catgggcgat
    42661 atccagcacc gatttgtccg agtggctgag ctggctgatc gccaggtcgc agcgcagatc
    42721 atccttgatg gactggtagg actgcccctc gtccgtcagg tggcgccgca gggacgaggc
    42781 ggagctgtgc agctggcgcg cgagggtgtc gaaatccggc caggcctccg gcggcaggcg
    42841 ccgtagcagg cgccggatgc gcgccgtgag gccttcgctg ttgcggtact tgaccaggaa
    42901 gttggccgga gcgctgcgca ggaagacctt ggccgtgcgc tcgttctgca cattggccat
    42961 gtccaggtag tccgccggaa agacgatggc ggtctccgcc tggtcaaaac gcaggtcggg
    43021 cgaaaagagc acccgccatt cgccgctgaa cgccggctcg gcgcagcgga agcgggcttc
    43081 ctgcagcggg atgcgccgcc cgatcagcca gcaggcgagg ccgtgcagga tgatcagata
    43141 ggtgccgtag gcaaaggccc ggcggggggc gctgcagcgg tcggcaatca cgaggtacgc
    43201 ccggtcgccg tcgcgcacca gcgtcccgga caggtcgtcg agcaccagcc gcaggaaacg
    43261 cagggcgcgg cgcagggctt tttcaagggt ctcgcagtgg atcagcgcat gacacagcag
    43321 ggtaaagctg cccacccgca tcgggtgact gtccatcccg aagaattcgt cgtcgagggc
    43381 ctgggcgatg aggtgccaca gggcgccgaa gtgggccgag gacacccgcg cctggggcgc
    43441 gttgaggagt tccggcgaaa taccggcccg attgagcaga tcctccgcat cgagcccgcg
    43501 ctggaccacc ccttccagcg cctcctggac gaaatggatg gagatggtgc ccttgtcggg
    43561 tggcggcgcg ttcatcgggc gtttgcaact ggcatggagg atcggcgcag cgaggatacc
    43621 acccaccaac accagtgcgc aggcaaccac cgcccgcccg gccgggggta tggcaaatcc
    43681 caccattcac attgaccgat ttcggcatgg aagccgaccg ggcgagcccc tagactggcg
    43741 ctcgcatcgt caccgggtct gcagcctcga cccgacaatc cacaagaccg aagaggagaa
    43801 aatgcatgga gatccaaaat aacgtattcg tggtcaccgg cggcggttcc ggcctgggcg
    43861 ccgccactgc ccgccagatc gtcgcccggg gcggcaaggt ggtgctggcc gacgtcaacg
    43921 cccaggcggg cgaagccctc gccgccgagc tgggcgccgc cgcccgcttc gtggccaccg
    43981 atgtgaccga cgaggccagc gcccaggccg cctttgccgc cgccaccgcg atgggccccc
    44041 tgcgcggtct ggtgaactgc gccggcgtgg ccccggccga aaaggtcgtc ggcaaggaag
    44101 gcgcccacaa gctggcgtcc ttcacccgca ccatcaccat caacctgatc ggcagcttca
    44161 acatgatccg cctcgccgcg gagatcatga gcaagagcga gcccgacgag ggcggcgagc
    44221 gcggcgtgat cgtgtccacc gcctcggtgg cggccttcga cggccagctc ggccaggctg
    44281 cctacgccgc gtccaagggc ggcgtggtgg cgatgaccct gcccatcgcc cgcgaactgt
    44341 cccgcgccgg catccgcgtc atgaccatcg ccccgggcat catggaaacc ccgatgctgc
    44401 tgggcatgcc cgccgacgtc caggacgccc tcggcaagat ggtccccttc ccctcgcgca
    44461 tgggcaagcc ggccgaattc gccgcactgg tggagcacat cctcggcaac cgctacctca
    44521 acggcgaggt gatccgcctc gacggcgcca tccgcatggc cgccaagtga cgcgccccgg
    44581 cgcatcgcga tcccatcccc tcccgaattg agaaaggtca tccccatgag caccgccaac
    44641 gaccccgtcg tcatcgtctc cgccgcccgc accccgatgg ggggcttcca gggcgatttc
    44701 tccagcctca ccgccgccca gctgggcagc gtcgccatca aggccgccgt ccagcgcgcc
    44761 ggcatctccg ccgaagcggt cgaggaagtc atctttggct gcgtgctgcc cgccggtcag
    44821 ggccaggccc cagcgcgcca ggccgccctg ggcgcaggcc tgtccctgtc ggccggctgt
    44881 accaccatca acaagatgtg cggctccggc atgaaggcca ccatgctcgc ccatgacctg
    44941 ctcctcgccg gtaccaacga ggtcatggtc gccggcggca tggagtccat gaccaacgcg
    45001 ccctatgtgc tgcccaaggc ccgcggcggc taccgcctgg gccacggcca gatcttcgat
    45061 cacatgttcc tcgacggcct cgaagacgcc tacgagaagg gccgcctgat gggcaccttc
    45121 gccgaggact gcgccggcct ctaccaattc acccgcgagc agcaggacgc ctacgccgtg
    45181 gcctccctca cccgcgccca ggccgccatc cgcgacggcg ccttcgcctg ggaaatcgag
    45241 cccgtcaccc tggccggcag gaagggcgaa atcctgatca aggatgacga acagccgccc
    45301 aaggccaacc tggaaaagat ccccagcctc aagcccgcct tccgcaagga cggcaccgtg
    45361 acgccggcca actccagctc catctccgac ggcgccgccg ccctggtgct gatgcgcgcc
    45421 tccagcgccg agcggatggg tctcaagccc ctcgcacgga tcgtggccca caccacccac
    45481 gcccaccagc ccagcctgtt cgccaccgcc ccggtcggcg ccatgcagaa gctgctggca
    45541 aagaccgggt ggagcgtggc cgatgtggat ctgtgggaga tcaacgaagc cttcgccgtc
    45601 gtcgccatgg ccgcgatcca tgacctgcag ctggaccacg ccaaggtgaa cgtccacggc
    45661 ggcgcctgcg ccctcggcca ccccatcggc gcctcgggcg cacggatcct cgtcaccctg
    45721 ctcggcgccc tgcgcaagac cggcggcaag cgcggcgtgg cgagtctgtg catcggcggc
    45781 ggcgaagcca cggccctggc gctggaaatg ctgtaaggca ggcagcctcc gacgtcgggt
    45841 tacgccctgc gggcgaaccc gacctacagg atcgcagcgc ccagccagca acggctcggt
    45901 gcccggccaa agcccgtctg aaccgccggg cggctcagca accggcgggg atttccggcg
    45961 cagctgtctg agcccggagg acgagttctg cgccggccgc ctgttgtgag ccgcgcaggg
    46021 gagtcagccg taggccgacc cgtgaagccg gggcgagacc cggccaacgc aacacccgac
    46081 aacggaaagc cgtcctgcaa gggcgcgcca gcgaaccgcc agcccgctca aacggcccca
    46141 cgaacaacgc aagagacacc aaaggaaacc acaccatgat cctcacccaa gaacaggaaa
    46201 tgatccgcga ctccatgcgc gccttctccc aggagcgcct cgcccccttc gccggcgaat
    46261 gggacaagca ccacaccttc cccgcccagg ccctcaagga gctgggtgaa ctgggcgccc
    46321 tgggcatggt ggtgcccgag gaatggggcg gcgccggcat ggactacatg agcctggtgc
    46381 tcaccctgga ggagatcgcc gccggcgacg gcgccacctc caccatcgtt tcggtgcaga
    46441 actccctggc ctgcggcatc accatgaagt acggcaccga cgcccagaag gaagagtggc
    46501 tcaagcccct ggcccgtggc gagaagctcg gctgcttctg cctcaccgag ccccacaccg
    46561 gctccgacgc cgccgccatc accacccgcg ccgaaaagga cggtgaccac ttcgtgctga
    46621 acggcgtcaa gcagttcatc accaccggca agcacgccca catggccatc gtcttcgccg
    46681 tcaccgacaa ggccgccggc aagaagggca tctcctgctt cctggtgccc accgccacgc
    46741 cgggcttcat cgtcggccgc accgaggaga agatgggtca gcacgcctcc gacaccgtgc
    46801 agatcatctt cgagaactgc cgcgtgccag cctcctccct gcttggcaag gaaggcgaag
    46861 gctacaggat cgccctgtcc aacctggaag ccgggcgcat cggcatcgcc gcccagagca
    46921 tcggcatggc ccgcgccgcc ttcgaagccg ccgtgcgcta cgcccgcgag cgggtgacct
    46981 tcggcgtgcc catcatcgag caccaggcgg tgaacttcaa actggcggac atgaacaccc
    47041 tgctcgacgc cgcccgcctg atggtgtggc gcgccgccca gctcaaggac gccggcaagc
    47101 cctgcctgaa ggaagcttcc atggccaaga tgttcgcctc cgaggccgcc gaaaagatcg
    47161 cctccgacgc catccagatc cacggcggcg tcggctacac cagcgacttc ccggtggagc
    47221 gcatctaccg cgacgtgcgc atctgccaga tctacgaagg cgccaacgac atccagcggc
    47281 tcgtgatcgg ccgctccatc gccgccgagt aaacggcgcg ccgggcgact gcgcggcccg
    47341 agcttgcgat tccggtacgc gccgtacgcg agtacgactg cgttcctcgt cgcaacctcg
    47401 gaccgctcgc aacgccctgc gcacccttta cgatgcgagc gttgtcgggg cgaacatcat
    47461 tcgccccagc cccgaagcgg cccgacctcc ggctaaagcc cgtctgaagc gccacgcagc
    47521 ggagcagtgg gcggggcctt ccggcgcggc tgtccgagct ggcaacgctg gcgagttccg
    47581 cgccgggcgc cggggcgaga cccggccaag ccgcgcaaaa cgggcgccgc cggctcaagc
    47641 gaaacaagaa caaaaaacca aggagacaac accgatgggc cccctccacg gcatcaaagt
    47701 catcgaattc gccgccctcg gcccctgccc gatggccgcc atgatcctgg ccgacctggg
    47761 cgccgaggtc gtgcgcatcg agcgcaagcc cgcgccgggc gccaagccgg cttctgacct
    47821 gttcgacccg gccatcgaca tcctcaaccg cagccgccgt gtcgtcaccc tcgacctgaa
    47881 gaagcccgaa gccctcgccg ccgcccgaca gctcatcgcc ggcgccgatg tcctcgtgga
    47941 gggcttccgc cccggcgtga tggaacgcct gggcctgggc ccggacgaga ccctcgcggc
    48001 caatccgcgc ctcgtctatg ggcgcatgac cggctggggg cagcacggcc ccctcgccca
    48061 ggccgccggt cacgacatca actacctgtc cctgtccggc gccctgcacg ccatcggcga
    48121 acccggcggc aagcccgtgg tgcccctcaa cctggtggcg gactgcggcg gcggcgccat
    48181 gctgctggtg gtgggcgtcc tcgcggcggt gctggaagcc cgccagtcgg gccgtggtca
    48241 ggtggtggac gcggccatga ccgacggctc ggccctgctc atgaccatga tgtacacctt
    48301 caaggccatg ggccaatgga cgcaggcgcg gggcagcaac ctgctggacg gcggcgcccc
    48361 cttctacgac acctaccgct gcgccgacgg caaatacctg tccattggcc ccatcgagcc
    48421 acccttctac gccctgttcc tggaaaaggc cggcctcacc gatcccgact tccggcagca
    48481 atgggaccgg gcgcgctggc ccgaactcaa gcagcgcgtg gccgcgcatc tcgccacccg
    48541 cccgcgggat gcctggtgcg ccctgctgga aggcaccgac gcctgcgtcg cgccggtgct
    48601 ggacatggac gaagcgcccg cccacccgca caatcgggcc cgcggcacct tcatcgaggt
    48661 gaacggcgtc gtccagcccg ccccggcgcc gcgcttcagc cgcagcaccc ccgccgcacc
    48721 cacaccgccc acgccgggcg ctcccggcga ggatgtactc gccgactggg gcctgagccc
    48781 ggacgccatc gccaccctca aggccgtcgg cggcctgtga tccgaatcca cccaaggaga
    48841 acccatgagc tacgaaaaca tcctcagcga agtccgcggc aaggtcggcc tcatcaccct
    48901 ccaccgcccc aaggccctca acgccctcaa cgacgacctg gtgaatgaag tccgccaggc
    48961 cctcgacgcc ttcgaggccg acgagggcat cggctgcatc gtcatcaccg gctccgagaa
    49021 ggccttcgcc gccggcgccg acatcggcgc gatggcgagc tactcctaca tggacgccta
    49081 caagggcgac tacatcaccc gcaactggga acgggtcaag acctgccgca agcccgtcat
    49141 cgccgcggtg gccggctacg ccctgggcgg cggctgcgag ctggcgatga tgtgcgacat
    49201 gatcttcgcc gccgacaccg ccaggttcgg ccagcccgag atcaagctcg gcaccctgcc
    49261 cggcgccggc ggcacccagc gcctgccccg tgccgtgggc aaggccaagg ccatggacct
    49321 gtgtctcacg gcccgcctga tggacgccgc cgaagcggaa aaggccggcc tggtggcccg
    49381 cgtcatccct gccgacaggc tgctggagga aacccttgct gcagccgagc agatcgccgg
    49441 ctattccctg ccggtggtca tgatgatcaa ggaatccatc aaccgcgcct acgagtccgc
    49501 cctcaacgaa ggcctgctct tcgagcgccg cctgttccac gcgagcttcg gcctggaaga
    49561 tcagaaggaa ggcatggccg ccttcgtcga aaagcgcaag gcggacttca agaaccgctg
    49621 agcgggacgc gcgtcccgac gccacggggg ctgcggcccc cgttttcgtg aacgccgccc
    49681 accgcggcgg gcggcacacg tcgcgttcgg aaatccggac gcctcaccct tcagccacgc
    49741 cgacgcccgg tgagcatcgc ccgcagcagg ctctccccct gcaggcggct caccagcgcc
    49801 accgccatca ggtgcagggc gacacaggcc atcagcccgt ccgccagcgc ctcgtggagt
    49861 tcgatgagcc cgtcgtcgcc ccagaaggcg tcaagccggc tcatccagcc ggtcaagccg
    49921 agcgccagga ccagcgccca catcaggtag gcggcccact gaccgagcgg gttgtgtccc
    49981 gccacggggc tcaggtgccc acgccccatc tcccgcaggt ggacaccgat cgccgtcaag
    50041 cccggacggg acagggccgc aggatcgccc gccggcatgc atgccccgta ggcgaggcgc
    50101 agcagcacca gggcgacggc cccatatccc gtggcgcgat gcaggggccc cgtctcgttg
    50161 aacagatcga acagcactgc cgccgccacg ccccagtggg tcaggcgcac cagtcggctc
    50221 cacaccatcg gaccgacagc catcgcggcg acccgcttat tcgatctcgg ccttcacgat
    50281 ggcaagggtc ttggtgtcga agtagatctc caccttcttg ccttcccggt tcttgccgta
    50341 gatctcgtag cagttgccgt ccaccttgaa cttgcggatc tggtagccct cggccaccag
    50401 gcgggccttg gcattctcct cggacatcca ttccgacttg ggatgcgctt cacagcgggc
    50461 acccgcgaag gcagcgagcg gggagagggt caggatggca aggatcaggg cacgcatgga
    50521 agctcctatc aagcaatgaa gtgaagtggg ttggcggtca gcctacacaa aacgataacg
    50581 attctcaata ccaaattcac catcggattt ccgaacaccc cgcggaccgc catccggatt
    50641 cccgaacagg cacgcccgcg cgcttcaaaa atttcatatt aaatcatcta cttgccgcac
    50701 ccgctcggtc accggccgca tggcacgccg tctgcaatac aaagcgcgtg ttcagccccc
    50761 ttcccattcg ccggagacga cccatgcgca ctcgctgttc cctcgccgct ccttccctgg
    50821 ccatcgccct tgcggtggcc gccctggccc agcccgccgc cgcccaggaa gtgcccaccc
    50881 tcgccaagat ca
//
LOCUS       DAOQMM010000012        50419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_145_length_50419_cov_51.212215, whole genome shotgun sequence.
ACCESSION   DAOQMM010000012 DAOQMM010000000
VERSION     DAOQMM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 50419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50419
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145_length_50419_cov_51.212215"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..898
                     /gene="urtE"
                     /locus_tag="PLH85_03555"
     CDS             206..898
                     /gene="urtE"
                     /locus_tag="PLH85_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNB63663.1"
                     /translation="MLTVDSLNQYYGGSHILRGLSFTAEPGQVTTLLGRNGVGKTTLL
                     KSLMGLVPTRSGSVTFGGQDLTHAPPHQRVRAGIGYVPQGREIFPRLTVEENLLMGLA
                     TKPGGTKIPERIFDMFPVLRQMLHRRGGDLSGGQQQQLAIGRALAMGPRLLILDEPTE
                     GIQPSIIKDIERAIRMLAQTGEMAILLVEQYYDFAESLADQYLLMERGEFILRGKGAD
                     MQKDGVREALSL"
     gene            907..1749
                     /locus_tag="PLH85_03560"
     CDS             907..1749
                     /locus_tag="PLH85_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNB63664.1"
                     /translation="MKPVEAPLAHGWHATLSLGFSRRETGTVLSDNRHRGPLRVQKAL
                     YPEGPAVCQTILLHPPAGIAGGDHLHIGVEAGPGSIAQLTTPGAGKWYRSAGPEASQR
                     LDFHVADGAALEWLPQETIVFDGARARMETRVQLSGNARYLGWDILCPGRAAAGERFR
                     QGRIDLHTRIERDGRPLWLERGGLLGDDPMFASPAGWDGAGVCATLLATGPVDADLLA
                     ACRAVAPADGARHAVTVLPGLLVARYLGDASEAARHWCAALWAVLRPALLGRPAVPPR
                     IWNT"
     gene            1765..2067
                     /locus_tag="PLH85_03565"
     CDS             1765..2067
                     /locus_tag="PLH85_03565"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNB63665.1"
                     /translation="MELTPREKDKLLIFTAGLLAERRKARGLKLNYPEAVALITCAIL
                     EGARDGRSVAELMSEGTKVLTRADVMDGVAEMIPDIQVEATFPDGTKLVTVHHPIV"
     gene            2078..2383
                     /locus_tag="PLH85_03570"
     CDS             2078..2383
                     /locus_tag="PLH85_03570"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNB63666.1"
                     /translation="MIPGELLAEPGELELNAGRPTITLVVANTGDRPIQVGSHYHFYE
                     TNAGLSFDREAARGYRLDIAAGTAVRFEPGQTRTVQLVALAGDRKVYGFRGLVQGAL"
     gene            2383..4089
                     /gene="ureC"
                     /locus_tag="PLH85_03575"
     CDS             2383..4089
                     /gene="ureC"
                     /locus_tag="PLH85_03575"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNB63667.1"
                     /translation="MANTISRQAYAEMFGPTTGDRLRLADTELIIEVEKDYTLYGEEV
                     KFGGGKVIRDGMGQSQRVSTETVDTVITNALIVDAVTGIVKADIGLKDGRIAAIGKAG
                     NPDIQPGVTIVVGPGTEVIAGEGMIVTAGGIDSHIHFICPQQIEEALNSGVTTMIGGG
                     TGPATGTFATTCTPGPWHIHRMLEAADAFPMNLGFLGKGNASLPEALREQVEAGVIGL
                     KLHEDWGTTPAAIDCCLSVADEMDVQVAIHTDTLNESGFVEATIDAFKGRTIHTFHTE
                     GAGGGHAPDIIKAAGLPNVLPSSTNPTMPYTVNTIDEHLDMLMVCHHLDPAIAEDIAF
                     AESRIRRETIAAEDILHDLGVFSMMSSDSQAMGRVGEVIIRTWQAAHKMKVQRGTLPE
                     DCTRHDNFRVKRYIAKYTINPALTHGIAHTVGSVEVGKLADLVLWRPAFFGVKPSLIL
                     KGGMIAAAAMGDPNASIPTPQPVHYRPMFGSFGKALKTSVTFVSQAALKNPAVAALGL
                     SKPLVAVSGTRTLSKADMVHNGATPEITVDPETYVVKADGVHLVCEPATELPLAQRYF
                     LF"
     gene            4103..4381
                     /locus_tag="PLH85_03580"
     CDS             4103..4381
                     /locus_tag="PLH85_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNB63668.1"
                     /translation="MTDPIARLANLGPKSSAMLAAAGIATVEDLRALGAVAAYARVKA
                     RDPHASLNLLWALEGALSGLPWQTVAREHRTSLLLALETCQTSPAPEC"
     gene            4375..4929
                     /gene="ureE"
                     /locus_tag="PLH85_03585"
     CDS             4375..4929
                     /gene="ureE"
                     /locus_tag="PLH85_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNB63669.1"
                     /translation="MLNLTQRTNAPATDAVALAYDDRKRSRLKVTLASGREAGIFLER
                     GEHLQGGDRLAGDDGATVVEILAAPEQLIEAEAGTPLLFARAAYHLGNRHVPVQLLPT
                     ASGGVLRFQTDHVLAEMVKGLGCTVAETDAPFQPEAGAYGAHGGHQHPYHPGEAGAAG
                     QADLHDPGHGPHRSVPKIHQFRAR"
     gene            4932..5138
                     /locus_tag="PLH85_03590"
     CDS             4932..5138
                     /locus_tag="PLH85_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNB63670.1"
                     /translation="MPYVNVQITRGATREQKAEIVRDITATLVRVLGKRPEHTHVVLQ
                     EIAEEDWGYAGLLTDEWKRQQGGA"
     gene            5138..5578
                     /locus_tag="PLH85_03595"
     CDS             5138..5578
                     /locus_tag="PLH85_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63671.1"
                     /translation="MRTGTLLRQTPLLVALLASSAPGAAVTPAYRGPDLSGTYVCTGH
                     DHQDGPYTGTVTLALLRTRSTGAQASYDFRLDVPGYGSYPGHAAAQGRHMAISFANTD
                     PTTHDYGTGIARFHRQHGKWAFDKFYHEPEYKGGNFGTEHCVQR"
     gene            5666..6352
                     /locus_tag="PLH85_03600"
     CDS             5666..6352
                     /locus_tag="PLH85_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNB63672.1"
                     /translation="MNDTLALARLLQLASPALPVGAYTYSQGLEWAVESGVIRDEAGA
                     ARWIGDLLAHAIGRFEAPLLARQMQAWRDTDHTEVLRLDADFLASRESAELRAETAQM
                     GFSLRRLLRDLRDEGLAPVALVVDPLAEVAFPTVWSGIAATWGIAPHAAVVAYLWSWA
                     ENQVMAALKAVPLGQAAGQRLLATLGTRLPALADAALDLPEAAWSNFTPAFALACARH
                     ETQYSRLFRS"
     gene            6355..6963
                     /gene="ureG"
                     /locus_tag="PLH85_03605"
     CDS             6355..6963
                     /gene="ureG"
                     /locus_tag="PLH85_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNB63673.1"
                     /translation="MTQQALRVGIGGPVGSGKTALTLALCQALREQIDMAVVTNDIYT
                     AEDAQFLVRHAALPPERIIGVETGGCPHTAIREDASINLEAVDRLQRAFPDLRLILVE
                     SGGDNLAATFSPELSDLTLYVIDVAAGEKIPRKGGPGITKSDLLVINKIDLAPLVGAS
                     LDVMAHDARAQRGERPFVFTNLKSGEGLDIVIRFIRDRGMLD"
     gene            7068..8561
                     /locus_tag="PLH85_03610"
     CDS             7068..8561
                     /locus_tag="PLH85_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63674.1"
                     /translation="MSASSPSLSERSAPILDWLASETSEDTDLEISALVRHLEEMAGS
                     EINRNQFYRCLELIHTRSLVIAAQMRQHLATGALPLTIGLHRSATRMIGALGKIVDGY
                     EQLLEETRNRLVRTTRRNPETLSAKALRLLGEQLTLSHLAGSAAPFDFWQRAHALFRL
                     SGRDAQTGDGAAEAPESVATAYKYLLCLAATQPEGLTGREICWLSDYIAEHANECRLR
                     DTAPTRNRAAWYWIDPDQNASPVSFSRREPPPVDGLLYFSAEELARGATELIDTLDGD
                     QAPTHDLPAWLRRGEAATLLRRVREYWAAPPHRAHTRRRNQYPITVCCGLESIWKMLR
                     APAGPRQNRFSTEWMVVNESPTGYAIMQVTGAAANLGPGIALALRRSEDEQWTICVVR
                     WIRTETPEQVELGLQVVANSAKPVTVGFRNSAKAQPMKPALVLPPLPALGKAQSILAP
                     GGTYTARRFMLVSDTQRLYVAQGRLLSLDMQTAAVELFQYEIDPYPL"
     gene            complement(8549..9817)
                     /locus_tag="PLH85_03615"
     CDS             complement(8549..9817)
                     /locus_tag="PLH85_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HNB63675.1"
                     /translation="MITDTHSLREGRLTAEAARHLAVAVWRRFHEGRCAQVAGSLTFT
                     SLLSLVPFVTLVVLTFSRFPQSTQFSEALKGFLLDNLLPDKAGKVIAAYAVQFSQKST
                     NLTIVGGVVLVVTALSLIKTIDQAINQIWNVRGTRRWATRFAVYWAALSVGPVFLAGC
                     VFASSAVLSLSLDLVGEPVWLGAVALRLMPVALLTGLFAAVYYIVPHCTVRPVDALAA
                     GFLAALLLVSMQRLFGLYLTHFTSYTLVYGAFAVVPIFLMWLYLSWIVILLGAVVAAV
                     LPERTLLGRMLPAFPGRSLYLALLVLGQLVRAQRRGLTPDVGHLARTARAGHDEVRAV
                     LESCQRARIVVRSDEGGWLLARAADAVSLAEVAQLFAWDGVPRKDAVATPLEAQIRPL
                     WETWVARLNVASELPLSVLAEAGDAKDQSG"
     gene            9854..10450
                     /gene="wrbA"
                     /locus_tag="PLH85_03620"
     CDS             9854..10450
                     /gene="wrbA"
                     /locus_tag="PLH85_03620"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNB63676.1"
                     /translation="MHEILVLYYSHRGAVRALAELIARGIDAVPGVAARLRTVPRVST
                     VCEATEGDIPERGAPYVEIRDLEECIGLALGSPSRFGNMAAPMKYFLDGTAGPWLSGA
                     LAGKPACVFTSSASPHGGQESTLLSMMIPLLHHGLLLVGLPFSEPDLTRTRTGGSPYG
                     ATHVAGPDDSNPISDEEKRLAVALGRRLAETALKLVAR"
     gene            10447..10815
                     /locus_tag="PLH85_03625"
     CDS             10447..10815
                     /locus_tag="PLH85_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNB63677.1"
                     /translation="MKPKTLSLIASLTLVTLIALCLAWETVLAPVRPGGSWMTLKVLP
                     LMAALFGILRGTRYTYQWSSMMILLYVTEGVVRASDPAPGGQLALAEAVLSIVFFVAV
                     VLYARNTAPSRLARAAQQDA"
     gene            complement(10849..12381)
                     /locus_tag="PLH85_03630"
     CDS             complement(10849..12381)
                     /locus_tag="PLH85_03630"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNB63678.1"
                     /translation="MNDQLIIFDTTLRDGEQSPGASMTREEKLRIARQLERMKVNVIE
                     AGFAAASNGDFEAVKAVAEAIKDSTVCSLARANENDIRRAGEAIRPARSGRIHTFIAT
                     SPIHMEKKLRMSPDEVVAQAVKAIGWAREYTDDIEFSAEDAGRSELDFLCRIFEAVIR
                     AGATTINVPDTVGYNVPEQFAATIRSLIERVPGSDKVVWSVHCHNDLGLAVANSLAAV
                     RAGARQVECTVNGLGERAGNASLEEVVMAVRTRKDIFLCDSRIDTTQIVPASKLVSGI
                     TGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWTANKLVLGKHSGR
                     NAFRSRLQELGIVIGSEEQLNHAFARFKELADKKHEIYDEDLQALLSDESYEPKEHFR
                     LVSSSFHSETGETPCAKLTLSVGGKEANSESCGSGPVDAAFKAIEVIADSGTQLLLYS
                     VNAITTGTDAQGEVTVRLSKDGKIVNGQGADTDIIVASAKAYLNALNKLHAKLERLNP
                     QV"
     gene            complement(12580..13362)
                     /gene="pssA"
                     /locus_tag="PLH85_03635"
     CDS             complement(12580..13362)
                     /gene="pssA"
                     /locus_tag="PLH85_03635"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNB63679.1"
                     /translation="MADPYARKPLLNPERRRRGIYILPNLFTTAALFAGFYAIVQAMN
                     GQFEQAAVAIFVAMVMDGLDGRVARLTRTQSAFGAEYDSLADMVSFGAAPSLIVYEWA
                     LKGMGKLGWIAAFIYCAGAALRLARFNTNIDVIDKRYFQGLPSPAAAALVAGLVWVVI
                     DNGLSGQDVRWVACTLTIFAGITMVSNVLFYSGKEINLRRSVPFFVVIAFVAGFALVS
                     SYPQGVLFGLFVCYAASGYVFAAWRWLGRRKAAVAGAEKSDL"
     gene            complement(13508..14524)
                     /gene="ilvC"
                     /locus_tag="PLH85_03640"
     CDS             complement(13508..14524)
                     /gene="ilvC"
                     /locus_tag="PLH85_03640"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNB63680.1"
                     /translation="MKVYYDKDADLSLIKGKQVTIVGYGSQGHAHAQNLRDSGVNVTV
                     GLRKGGASWSKAEGAGLKVAEVAEAVRAADVVMILLPDENIPEVYRNDVEPNIKQGGV
                     LAFAHGFNVHYNQVVPRADLDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVYQDKSGKA
                     RDIALSYAAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKMGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEFGEYVTGTEVINEQSRAAMRN
                     ALKRIQTGDYAKMFILEGRTNYPAMTARRRLNAVHPIETVGAQLRDMMPWIKANKLVD
                     QSKN"
     gene            complement(14573..15064)
                     /gene="ilvN"
                     /locus_tag="PLH85_03645"
     CDS             complement(14573..15064)
                     /gene="ilvN"
                     /locus_tag="PLH85_03645"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNB63681.1"
                     /translation="MRHVISLLIENESGALSRVSGLFSARGYNIESLTVAPTEDASVS
                     RMTIVTSGSDEIVEQITKQLNKLIEVVKVVDLSESAHIERELMLVKVRATGKDRDEMK
                     RLSDIFRGRIIDVTESTYVIELTGTQAKLDSFVGAIDPALILETVRSGICGVGRGDRV
                     LKV"
     gene            complement(15165..16901)
                     /locus_tag="PLH85_03650"
     CDS             complement(15165..16901)
                     /locus_tag="PLH85_03650"
                     /inference="COORDINATES: protein motif:HMM:NF005409.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HNB63682.1"
                     /translation="MAETASGAAMVLTGAEIVIRCLQEEKVDYLFGYPGGAVLYIYDE
                     IFKQDKVRHVLVRHEQAAIHAADGYARSTDRVGVALVTSGPGATNAVTGIATAYCDSI
                     PMVVISGQVPSAAIGQDAFQEVDTVGITRPCVKHNFLVRDVREIAPTLKKAFYLARTG
                     RPGPVLVDIPKDITAQKCEFVYPASVSMRSYNPVVKGHHGQIRKALQLLLEAKRPMVY
                     AGGGVVLGDASEKLTNLVRRLGFPCTNTLMGLGGYPATDRQCLGMLGMHGTYEANMGM
                     HYCDVLLAVGARFDDRVIGNPDHFASEPRKIIHIDIDPSSISKRVKVDVPIVGDVGEV
                     LGELLALLEGTSERPDAEALDVWWKQIEEWRRRDCLKFTQPDDIIKPQFVVQKLWEAT
                     GGDAFVTSDVGQHQMWGAQYYKFDKPRRWINSGGLGTMGFGMPAAMGVQLAHPGSPVA
                     CITGEASIQMNIQELATCKQFRLPIKVVMLNNRYLGMVRQWQEIFHGNRYSESYMDSL
                     PDFVKLAEAYGHVGIRVDKPADVEGALREAFQRKNDLVFLDFQVDPTENVYPMVQGGK
                     GLTDMILSAEDL"
     gene            16990..17598
                     /locus_tag="PLH85_03655"
     CDS             16990..17598
                     /locus_tag="PLH85_03655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB63683.1"
                     /translation="MSGYSSASPARNSPLSSRIELSDFLASVEKRAFKQALFAVRNEE
                     TALDIVQDAMMKLAEKYGDRPPEELPMLFQRILQNVIRDHYRRQKVRSMWTTLLSAFS
                     GPDEEDEHDPLETLESADSEFRSPSPQAEVEQQQTLSLIEREIEKLPPRQREAFLMRY
                     WEEMDIAETAAAMGCSEGSVKTHCSRATHALAAALAAKGITL"
     gene            17595..17966
                     /locus_tag="PLH85_03660"
     CDS             17595..17966
                     /locus_tag="PLH85_03660"
                     /inference="COORDINATES: protein motif:HMM:NF023699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HNB63684.1"
                     /translation="MIEHEIHRRLRQHLNRSLQRIEPDTSERLHAARRKALSRQKVAV
                     HGLSLAGAGRLITDHLPDRRTAVAMVLVVVIALASGFLGDLQRTADIEEVDSSLLADD
                     LPIDAYLDRGFDTWVQSQALE"
     gene            17939..18559
                     /locus_tag="PLH85_03665"
     CDS             17939..18559
                     /locus_tag="PLH85_03665"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNB63685.1"
                     /translation="MGAKPSSRVIAALALCWAAHAAAVPPLKAPSWNELTPAQQRTLA
                     PLVKEWSNLPDDRKRKWVGIADRYPGLTPEEQARLQQRMHEWASLTPAQRESAREQYR
                     NLQKMPPEQRQALREKWEAYLELPEEEKKRFTDAAPKPGPKPPVPGQPATPIKPLTAP
                     PQPARPVVLPKPVPPPAPLTAPAPVPVPVPAQAPVVAGQDPTPPKP"
     gene            18563..19033
                     /locus_tag="PLH85_03670"
     CDS             18563..19033
                     /locus_tag="PLH85_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNB63686.1"
                     /translation="MMPSTATSGATPRPTGELATLRRRLASMLYEVLLLLGVLALTFL
                     VPLLILGIGFGILPPGGVMWLWIYLALGGYFVWYWRQGGQTLAMQTWKLRIVAANGDT
                     VSVRQAWLRYSLAWASLCVGGLGLFWALIDPERQFLHDRLAGTRIVLLLAPPAD"
     gene            complement(19041..20117)
                     /gene="lptG"
                     /locus_tag="PLH85_03675"
     CDS             complement(19041..20117)
                     /gene="lptG"
                     /locus_tag="PLH85_03675"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNB63687.1"
                     /translation="MAPIFVRYLAREIYAAVAMVLLAFLGLFAFFDLVNELDDVGKNG
                     YMLYHAALYVAMILPGRVYELMPIAVLIGTLYALTTLARHSEITVLRASGLSTGRFLA
                     ALLTIGSVFVAITFVFGEYLAPPAERAAQQWRLAATRSMVSQELRTGLWVRDGHRFVN
                     VRNVMPDTTLEGVRLYEFDDRHALVSISEATRGVFDERNGWRLTEVAQTRFLGDRTET
                     VSLPELVWHSELTPDVLSVLMVVPERMSVTTLYSYIRHLEDNNQRTTRYEIALWKKLV
                     YPFAALVMMGLALPFAYMQNRMGGVSLKVFSGIMIGVGFHLLNGLFSNLGVINGWVPA
                     VAALTPSVTFLVAAATMMWWVERR"
     gene            complement(20117..21193)
                     /gene="lptF"
                     /locus_tag="PLH85_03680"
     CDS             complement(20117..21193)
                     /gene="lptF"
                     /locus_tag="PLH85_03680"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNB63688.1"
                     /translation="MIFHRAAQREFTQNAVAVFVALFAILISTVLIRLLGQAAGGRVP
                     ADAVLALIGFGAIAQMPVLLSLTVFIAILMSLSRAYRDSEMVVWFSCGLPLTAWVRPV
                     LRFALPVVIVVALLSLFGAPWAQKKSVEYKEQLNNRDDVSRVAPGMFRESSGGERVFF
                     VEAASGEEGRVRNVFASSMQNGRLGVMVAANGHSEVDGLGNRFVILEHGRRYEGVPGT
                     PSYRVMEFERYQIRMQSKEAVTGPRLPKTLTFPALLKEKGNLYRGELLARLSTPVLAV
                     MLALLAIPLSFVNPRAGRTNNLILAILVYLIYSNALSVSQAWVAQGRLGFEVALMAPH
                     VVMMLLLAVLFWKRLSVFARWRRR"
     gene            21320..22807
                     /locus_tag="PLH85_03685"
     CDS             21320..22807
                     /locus_tag="PLH85_03685"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNB63689.1"
                     /translation="MEFTIKAGSPEKLKSGCVVAGVFAGGELSAAAKTLDKASSGALA
                     TILARGDLDDKAGATLMLHGVPGIEAPRVLLVSLGAASELTDKRYRDAVAAAARQLAA
                     SMAGDAAFCLAEADVPGRDLAWRIAQAAQILQDSTYRCDELKSRKEDSKKGVVHVAFI
                     TRAKHGDELQAALRRGEAIAEGLALAKTLGNLPGNVCTPGYLATTAKKLAKEHKIKVE
                     ILDRDAMEKLGMGALLSVARGSHEAPRFIILQYKGGKAKDKPVVLVGKGITFDTGGIS
                     LKPGEGMDEMKYDMCGAASVLGTFKAIARMELPLNVIGLIPSTENMPGGSATKPGDIV
                     TSMSGQTIEVLNTDAEGRLILCDALTYAERYEPAAVVDIATLTGACIIALGNLTSGLL
                     ANDDALAAELLAAGQSAGDKAWQLPLWDEYQDMLKSNFADIPNIGSKGAGTITAACFL
                     ARFTKAYTWAHLDIAGTAWKSGADKGATGRPVPLLVEFLARRAGA"
     gene            22822..23253
                     /locus_tag="PLH85_03690"
     CDS             22822..23253
                     /locus_tag="PLH85_03690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNB63690.1"
                     /translation="MPEIRFYHNAPDRLRVACSVTAKAWGQGRRISVLATDGAQARHF
                     DQLLWSFQPLAFVPHVGAESPLAAETPVVIGTHLDALHHEDVLINLGGELPPGFERFG
                     LVVEIVGQDEFERHAARQRFRAYKQAGFPLHAHDLAQRSGA"
     gene            23250..23687
                     /locus_tag="PLH85_03695"
     CDS             23250..23687
                     /locus_tag="PLH85_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63691.1"
                     /translation="MSDSHRPVLEVPLEDEDGEHLLAKADALMRRHGHFAAPAEPDDL
                     PVLTDVVDYPGEEAPPDDSPPDITPVALPNARDDQTARIVDLEAAITRSIDAWVSTEL
                     PQIMSREFDAFAERLREAAREHMRTTLLPTLTAQIEKTLDTEE"
     gene            23761..26637
                     /locus_tag="PLH85_03700"
     CDS             23761..26637
                     /locus_tag="PLH85_03700"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNB63692.1"
                     /translation="MELAKSFEPADIERRWYQHWESSGYFAAGLDRSKPADQSFCILL
                     PPPNVTGTLHMGHGFNQTLMDALTRYHRMRGWNTLWQPGTDHAGIATQIVVERQLDAQ
                     GVSRHDLGREKFLEKVWEWKDYSGGTITRQMRRLGTSPDWSRERFTMDAGLNKIVTET
                     FVRLYREGLIYRGKRLVNWDPKLHTAVSDLEVVQEEEDGFLWHLNYPLADGSGHLTVA
                     TTRPETMLGDTAVMVHPDDERYAHLIGKTVRLPLVERDIPIIADAYVDREFGTGVVKV
                     TPAHDFNDYAVGLRHGLPMIIVLTLEGAIPATGTVVEGGVATGSAPLPADLVGLDRFD
                     ARKAVVERFEALGLLGEVKPHKLQVPRGDRTNAVIEPMLTDQWFVAMSKAGADGRSIT
                     EKALEVVASGEIKFVPENWVNTYNQWLNNIQDWCISRQLWWGHQIPAWYGEDGQTFVA
                     HDEAEARAHATAAGYSGPLSRDEDVLDTWYSSALWPFSTLDWTPDYPARSNDALDLYL
                     PSTVLVTGFDIIFFWVARMVMMTTHITGKIPFRDVYVHGLIRDGEGQKMSKSKGNVLD
                     PIDLIDGIGVDDLVAKRTTGLMNPRQAASIEKKTRKEFPNGIPAFGTDALRFTFASLA
                     SPGRDIKFDMNRCEGYRNFCNKLWNATRFVLMNVQGHDLGLEHQQNGPSCGGNGPLAF
                     SFADRWIVSRLQRVEQDVALHFAEYRFDLVSKAIYEFVWDEYCDWYLELAKVQINTGD
                     EASQRGTRRTLIRVLETVLRLAHPLIPFVTEELWQTVAPLAGRKDTESICLARYPEAD
                     LGRCDETSEGDVAELKALIHACRNLRGEMNISPAQRMPLVACGNATRLTRFAPYIAGL
                     AKLSEVQVVDEIGSDELAPVAVVGEQRLMLKVEIDIAAERERLGKEIARLEGEIAKAK
                     AKLANESFVARAPAAVVAQEQERLAAFGATLDTLRPQLAKLSAA"
     gene            complement(26741..28114)
                     /locus_tag="PLH85_03705"
     CDS             complement(26741..28114)
                     /locus_tag="PLH85_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNB63693.1"
                     /translation="MAIKWLIDASYFVAAVLFILGLKGMSSPVTARKGIVWAGVGMVL
                     ATVVTFATPGMQNYLLMVIAIAVGGALAWISGKKVAMTDMPQMVAIYNGMGGGAAAAI
                     AAVEFARGEMHGAVVTLLATLGALIGAVSFAGSVVAWAKLQGVMKKAFRLPAQNAVNV
                     LLALLTVGLGAAIVIAPAVDPTLVGAFFILALILGTIITLPIGGADMPVVISLFNAFT
                     GLAVGFEGFVMGIPALIIAGIVVGAAGTLLTQLMAKAMNRPISNILFVPMAASGGGEA
                     VEGEQKEVGAMDAAAMMRYASKVIIVPGYGMAVAGAQHKVWEMTELLEEAGVEVSFAI
                     HPVAGRMPGHMNVLLAEAGVPYDKIFDLEEINADFAQADVALVIGANDVVNPSARTDK
                     SSPIYGMPILNADLAQNVIVVKRGKGTGYSGIENALFYKDNCRLLYGSAQQAVGEVIQ
                     HVKAMSV"
     gene            complement(28114..28398)
                     /locus_tag="PLH85_03710"
     CDS             complement(28114..28398)
                     /locus_tag="PLH85_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNB63694.1"
                     /translation="MDGFVALYIFMLAAFTGYEVISRVPVILHTPLMSGSNFVHGVVL
                     VGGMIALGQAQTPLEQAIGFVAVLLGAANAAGGYVVTERMLAMFKSKEKN"
     gene            complement(28412..29536)
                     /locus_tag="PLH85_03715"
     CDS             complement(28412..29536)
                     /locus_tag="PLH85_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNB63695.1"
                     /translation="MPLVIGVTKEFTPGERRVALVPDVAKKYQGLGASLLLESGAGEP
                     AYFRNSDYAGSDVADDAAAVLRKADVVLQVQPLGLDALRELRPGSVLVGLQQPWSSAE
                     RIAVLRERNITCFALELLPRISRAQSMDVLSSQAAVAGYECALIAADHCPKFFPMLTY
                     AAGTIRPAKVLVIGAGVAGLQAIATAKRLGAMVSGYDVRPETKEQIESLGAKFVDTGV
                     SASGSGGYARELTDEEKKQQAEKLGKAIGECDALITTAAIPGKKAPVIVSAEMVARMK
                     PGSVVVDMAAETGGNVAGTVPGEKTWVGEVLIIGPTHIPSRMPVHASEMYAKNLFNFI
                     SPFIKDGALALDWEDEVMAGACLTHAGELRHAGVKQALGL"
     gene            29780..30781
                     /gene="corA"
                     /locus_tag="PLH85_03720"
     CDS             29780..30781
                     /gene="corA"
                     /locus_tag="PLH85_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNB63696.1"
                     /translation="MKTEKPSITLLEYDSTGISEIRFDSIEESRNFTRSREKIWLNVY
                     GLHDPAIMAEIGRRFRLHPLIQEDILNTQQRPKVDEFAEYLYLVARYFDYDAPARCLT
                     SDQISIVLGRNFVLTFQERATGAFEPVRERLRAETGAMRAHGPDYLAYALIDTLVDRY
                     FIMLDHLSDLAEELEESALTRPTPSLLAEINRVKHEARSMRRAVWPLREVLNTLSRAD
                     NDFFTADTRLYLRDIYDHTVHVLESLESVRDLLADLLDIYLSSVSNRLNTEVRILTVL
                     TTLFMPATLIAGIFGMNFHEMPLLADADGFWVAMGLMGGAAAFMAASFWRRNWLKTQ"
     gene            complement(30804..31634)
                     /locus_tag="PLH85_03725"
     CDS             complement(30804..31634)
                     /locus_tag="PLH85_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNB63697.1"
                     /translation="MLEQSLASIPAPDTVTAKKRYQAEACVAKHVGDRTEQQDRVAVF
                     AHPARKGTLLAVLADGMGGHSGGAMAAEQVVLKAKQNFETFAPASESPEDLLRSVVDE
                     AHIVIKLTRFTSEQDPHSTAVVFMMQPDRADWAHCGDSRFYHFRNGKLMTRSADHSLV
                     EELVRKGRLDAESAARHPNRNVLLSCLGADREPRVDLGHVSPLQGGDAFLLCSDGLWG
                     YISEDEFGDVLASHTPRDAASTLIEMARHRARGGGDNISLAIVKLVELPPKTVPPRES
                     "
     gene            complement(31815..33071)
                     /gene="glcF"
                     /locus_tag="PLH85_03730"
     CDS             complement(31815..33071)
                     /gene="glcF"
                     /locus_tag="PLH85_03730"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HNB63698.1"
                     /translation="MQTQLADFIRDTPEGREAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGQPATARTRLHLDRCLTCRACETTCPSGVEYGRLADIGRLVV
                     ERQVPRQGVDRLGREALRQLVPNTGLFGLALGLGRRARPLLPRLLADKVSAADPAGEW
                     PAARHPRRMIVLAGCVQPSLRPAINAAAARVLDRLGVSLVEQAGAGCCGAVKFHLHDQ
                     EGARMEARRNIDAWWPGVAAGRVEAIVMTASGCGTQVKDYGHLLQHDPAYAEKAVRIA
                     ALCRDLSEVLAAEGDALETLIAGSAAAVERRRIAFHAPCSLQHGQKIRGVVEKLLEAA
                     GFELAPVRDAHLCCGSAGTYSLLQPTLAGRLRADKLDALVDSGGATVVSANIGCIEHL
                     AAGGRLPVKHWIELIDEYFTALRQKI"
     gene            complement(33075..34139)
                     /gene="glcE"
                     /locus_tag="PLH85_03735"
     CDS             complement(33075..34139)
                     /gene="glcE"
                     /locus_tag="PLH85_03735"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HNB63699.1"
                     /translation="MTDPIQEWAARIRKAAADGSAVCLRGGGTKDFLGRTPMGEVLDT
                     RGYTGIVSYEPSELVVTARAGTPLVELEAALAEAGQMLAFEPPRFAPGGTVGGCVAAG
                     LAGPRRAAVGGVRDFVLGVRLLDGRGDDLRFGGQVMKNVAGYDVARVMAGSLGTLGLL
                     LEVSLKVLPRPLAEATLAFALDEPAALERVNAWSGQPLPVSATAWRAGRLHVRLSGAA
                     AAVEAAIRRLGGEVLADTAAHGLWAALRDHTDDFFTDDRPLWRLSVPSDAPPLDVPGE
                     TLVEWGGAQRWLKGEQSAAELRARVAAVGGHATLFRHGDRAGEVFHPLLPAMAALQRR
                     LKQTFDPAGVFGPGRLYGGM"
     gene            complement(34132..34299)
                     /locus_tag="PLH85_03740"
     CDS             complement(34132..34299)
                     /locus_tag="PLH85_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63700.1"
                     /translation="MDALPEGWLQFLFVAVPVLSLIGTWMGLRTAGRIVERRERARRV
                     GQHPGSGDTCD"
     gene            complement(34305..35822)
                     /locus_tag="PLH85_03745"
     CDS             complement(34305..35822)
                     /locus_tag="PLH85_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNB63701.1"
                     /translation="MTADAHTFPDFSERERDFSRVDKHRLVTALSRALPAHGLLHEPE
                     DLRPYECDGMSALRQLPLVVALPDTEAQVVEILRICAAQQVPVVARGAGTGLSGGAQP
                     HRFGVVLSLARFKKILHIDAHARRAVVQPGVRNLAISEAAAPYGLYYAPDPSSQIACT
                     IGGNVAENSGGVHCLKYGLTVHNLLKVRAVTIDGEIVEFGSLALDAPGYDLLPLVTGS
                     EGMLAVVTEVTVKLTPKPQLARCVLAAFDDVVKAGDAVARIIAAGIIPAGLEMMDRPA
                     TAAVEEFVHAGYPLDAEAILLCEADGTPEEVAEEIERVRAILEGSGATEVRVSRDEAE
                     RLRFWAGRKAAFPAAGRISPDYYCMDGTIPRKRLGEMLRAIQAMEKKYGMRCINVFHA
                     GDGNLHPLILFDANTPGDQDVAEAFGAEILELSVALGGTITGEHGVGIEKINQMCSQF
                     ATPEIRLFHRVKAAFDPLGLLNPGKAIPTLNRCAEYGRERMGRGVPDAHPDLPRF"
     gene            complement(35819..36565)
                     /locus_tag="PLH85_03750"
     CDS             complement(35819..36565)
                     /locus_tag="PLH85_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNB63702.1"
                     /translation="MSVVPLTPVLRPGRLAEEVGADLERRIRRGDFPPGGRLPSESQL
                     AAQFGVSRTVVREAIARLRAEGLVDSRQGAGAFVMQRPGAASFRLRDVSAEPCSDAEV
                     FELRLVVEAAVAERAARRHTAADLAAIAGALARMDAALRAGEGGAEADDAFHRAIAAA
                     CGNALLQRLVEFVSQDLSASRRPTWSRDGLIAGRALRAQDEHRALFEAIAAGDGAAAR
                     RAAEQHLIGTAARLGVSWSLDPASPSKEPT"
     gene            complement(36745..37530)
                     /locus_tag="PLH85_03755"
     CDS             complement(36745..37530)
                     /locus_tag="PLH85_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HNB63703.1"
                     /translation="MLNFSANLSFLFLERPFLERFEAAAACGFRGVEFHFPYAFPVDE
                     VVDAARRAGVEVVLFNVPAGDWAGGERGLGCLPERRTEFRDGVGLAATYAAALACPRV
                     NVLAGLRPSGVADAVLRATLIDNLRYAADRLSESGRTVLLEPLNTRDTPGFFVSRSHD
                     GLALLAEAGRPNLRLQYDVYHAQVMEGDLARTLGEHLAQIGHIQLADNPGRHQPGTGE
                     IRFPFLFGHLEALGYAGWIGCEYVPTGRSEDSFAWREDWVDRG"
     gene            37663..38997
                     /gene="pcnB"
                     /locus_tag="PLH85_03760"
     CDS             37663..38997
                     /gene="pcnB"
                     /locus_tag="PLH85_03760"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNB63704.1"
                     /translation="MIRKLLRRVFRRAAPANALPEPAVIPAARHGIRRDQLSPAAIKV
                     CTVLQDAGHKAYVVGGAVRDLIVGQTPKDFDVATSATPEEVRALFRRSRIIGRRFKIV
                     HVMSGPETIEVSTFRAMQDAESTETDEHGRVLRDNVFGSMAEDATRRDFTVNALYYDP
                     SDETVVDYHHGVADLQQRTLRMIGDPRVRYREDPVRMLRAARLGAKLGLGIDPAARGP
                     IREMADLLENVPPARLFDEMLKLLLSGHAVKCLKQLREEGLHHGLLPLLDVILEQPLG
                     ERFVWLSLENTDERVRQGKPVSPGFLFATLLWHEVLADWEARKADGQHAQPALFEAMD
                     AVLDKQSGKLAITRRIVGDIKDIWALQPRFDKRAGKAPYRLLEQARYRAGWDFLRLRA
                     QAGEIPMELPDWWDAFADAHPDAREAMLLPEKAGSAPRKRRRRKKPAAAISE"
     gene            38994..39500
                     /gene="folK"
                     /locus_tag="PLH85_03765"
     CDS             38994..39500
                     /gene="folK"
                     /locus_tag="PLH85_03765"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNB63705.1"
                     /translation="MSSAVSAGTRAYVALGANLGDPVPTLRAAVADLGALPGTSVVIR
                     SSLYRSAPVGLVNQPDFFNAVVAVDTSLPALDLLRGLLAVEARHGRVRSVPNAPRTLD
                     LDLLLHGDQACSSAELILPHPRMHERAFVLLPLLEIAPDVWIPGRGPAREALPAVAGQ
                     AIARAEAL"
     gene            39500..39841
                     /locus_tag="PLH85_03770"
     CDS             39500..39841
                     /locus_tag="PLH85_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNB63706.1"
                     /translation="MPIWLQTALLLAASNVFMTFAWYGHLKNLASAPWYIAALVSWGI
                     ALFEYLLQVPANRIGARQLDLPQLKILQEAITLTVFVPFAVFYMQQPLKLDYLWAALC
                     ILGAVYFIFRT"
     gene            39857..40495
                     /locus_tag="PLH85_03775"
     CDS             39857..40495
                     /locus_tag="PLH85_03775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB63707.1"
                     /translation="MLEKARYIVVEGPIGAGKTTLAKRLAERLHADLLLENPATNPFL
                     GRFYQDAERWGLATQIAFLFQRSEQLALFTRDHDPFRRAVSDFLLDKDPLFAELNLDV
                     DELQLYRQLFERLKPTVRQPDLVIYLQAQPDILIERVRKRGVEAERKISESYLQRVAE
                     HYARFFHHYDAAPLFIVNAESLNPVDKDEDFELLYSRLLAMRSYREFFGYAG"
     gene            40620..41438
                     /gene="panB"
                     /locus_tag="PLH85_03780"
     CDS             40620..41438
                     /gene="panB"
                     /locus_tag="PLH85_03780"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNB63708.1"
                     /translation="MTYLQDTAKPVTLFELGKMRAEGRRIVMLTGYDASFASLLERCG
                     VDVILVGDSLGNVLQGQKTTLPVTMEHMVYHTECVARGCSRPFIVADMPFGSYAEGPE
                     QAIRNAARLMAAGAQMVKLEGGAVMAETVRFLVERGIPVCAHIGLTPQSVHQLGGYRV
                     QGRSDSGAATLKADAFALEQAGAALMVLEMIPAALATDLTRSLTTMATIGIGAGVDCH
                     GQVLVLHDLIGVFPGRRPRFAKNFMEGAASLDDAVTRYVTDVREGRFPGPEHSY"
     gene            41446..42270
                     /gene="panC"
                     /locus_tag="PLH85_03785"
     CDS             41446..42270
                     /gene="panC"
                     /locus_tag="PLH85_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNB63709.1"
                     /translation="MQIHPTIAGLRAALAGAGRVAFVPTMGNLHDGHIALMREARRHA
                     DVVVASIFVNRLQFGPREDFDKYPRTFADDCARLTDAGVDHLFAPDETEMYPAPQRYH
                     VEPEPAQVSILEGAVRPGHFRGVATVVLKLFNIVQPQVALFGKKDYQQLMVLTNMTRE
                     LALPIRIVPGETIRAADGLALSSRNGYLSAAERAEAPRLFAQLSRIRERLQAGERDLA
                     RMEADAVAELAAHGWQPDYIAIRRRSDLQAPEGDEPLVVLAAARLGSTRLIDNLEL"
     gene            complement(42334..42813)
                     /locus_tag="PLH85_03790"
     CDS             complement(42334..42813)
                     /locus_tag="PLH85_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63710.1"
                     /translation="MYVALFCLIGIVALAAISAQAWRDRARVAASVTPDWHDTLRRLA
                     TEQELGHYRSAREKTVSVLRRLESRRFDPRMAAHVRVRIASLMARDPVYTEVVRAIRK
                     ACAAQTSISEMALCVGLRQFLIEDIRISMEIAEMLGQVRRAEEGGSPTVMAEFHPTA"
     gene            complement(42986..44152)
                     /locus_tag="PLH85_03795"
     CDS             complement(42986..44152)
                     /locus_tag="PLH85_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63711.1"
                     /translation="MKRIPLAYSLRNLWARRLTTALTAGGMALVVFVFAAVLMLDQGL
                     KEALVDTGQADNVVVIRKGSETEVQSIIERNQAALLETLPQVALAAGGLPQVSKETVV
                     LITLPKHGGKPANVIIRGVGGQGAALRPTLALVEGRMFRPGSAEIVVGASIARRFDGT
                     GLGSTLRFGARDWRVVGVFEADKSGFESEIWGDADQLMQAFRRPAYSSVVLRLADPAS
                     FPAVRALIDDDPRLSLEAKTERAFYADQSAMLSNFISILGMTLSVIFSVGAMIGAAIT
                     MYAAVATRTAEIGTLRALGFRRGSVLLAFLTESLLLALLGGAAGLAAASLMQFFTIST
                     MNWQSFSELAFSFRLTPAIVVKTLIFALAMGLIGGFMPAARAARMNIVSALRDA"
     gene            complement(44156..45319)
                     /locus_tag="PLH85_03800"
     CDS             complement(44156..45319)
                     /locus_tag="PLH85_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63712.1"
                     /translation="MLTAFLRMILRNAWRHRLRAGLTVLGMVVAILAYGLLGTVVDAW
                     YAGAEGASANRLITRSSISLVFPLPLTYRDRIRGVEGVRDLSWASWFGGIYKEPKNFF
                     AQFAVDGESYFRIYPEFVVDERAFRDFLRDRRGCVVGRKLAETYGFKVGDNLTLKGTI
                     YPGNWDFVVRAIYDGARKGTDQSQMLFHWQYLDETIKQRYPGRANNVGVFVTEVASPD
                     QVAAVSRAVDREFANSRAETLTETEKAFQLSFVAMTEAIVVAIRIVSYLVIFIIMAVM
                     ANTMAMTARERTAEYATLKALGFAPAFVGLLILGESLMLALTGGAIGIALTFPVADAF
                     AAKMGTLFPVFNVSTETVALQIACALTVGMVAALLPMLRVGRLRIVDGLRAVG"
     gene            45469..45912
                     /locus_tag="PLH85_03805"
     CDS             45469..45912
                     /locus_tag="PLH85_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB63713.1"
                     /translation="MATTVQQILNNKGGTCHGVAPDTTVLEALKLMAERNISAVLVTE
                     GPRLVGIFTERDYARKVVLRGLASKDTKVGDLMTQNVLTVSPSQTVEEVMGIMTNNHI
                     RHLPVVDHGVLAGIISIGDAVKSVMEQQQATIEQLAGYIAGGMST"
     gene            complement(46002..46721)
                     /locus_tag="PLH85_03810"
     CDS             complement(46002..46721)
                     /locus_tag="PLH85_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB63714.1"
                     /translation="MTDARGSAAGAPIVRLRDVRKAYRRGAQVVPVLAGIGFDIAEGE
                     FLALMGPSGSGKSTLLNLIAGIDRADSGELIVAGEDIARLAERELADWRARHIGFIFQ
                     FYNLMPVLDALENVELPLLLTTLSARERRERAAFALSMVGLADRMDHTPGELSGGQQQ
                     RVAIARALVTDPTLIVADEPTGDLDRESATEILGLLQRLNREAGKTIVMVTHDQRAAA
                     AAHAIMHLEKGELSGREAVRT"
     gene            complement(46718..47923)
                     /locus_tag="PLH85_03815"
     CDS             complement(46718..47923)
                     /locus_tag="PLH85_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB63715.1"
                     /translation="MSESDLSRLSIDRNAPRPSRRRARAWLIGGALVVVAALAAAIGH
                     FNAPVSVETVSVGEAWPYQGVTVLNATGYVVAQRKASVASKATGRLEWLGVREGSVVK
                     AGELIARLEQRDVVAQAEQAAANVGVARANVGQARAELKDAELALGRARDLRARNFVA
                     QAQVDQAEARATKARAALGSAQAAETAAVAAERAARVAVAQTEIRAPFDGVVLTKNAN
                     VGDLITNFSAAADSKGAVVSMADMGTLEVEADVSEANLGRIRVGQPCEIQLDAFPEQR
                     FRGEVSRRVPTVDRAKASVLTKVRFLDPDPRVLPEMSAKIAFLDKALADAERTPRVAV
                     HEDALRREGSEVALFRVEDGRAHRVVVQTGATIGELVAVQGVKSGDKVVLRPPESLRD
                     GAAVKAGGK"
     gene            complement(47998..48600)
                     /locus_tag="PLH85_03820"
     CDS             complement(47998..48600)
                     /locus_tag="PLH85_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB63716.1"
                     /translation="MPIDPSATFPVHPRPWAGALLRRLGWTLDVAPLPAAHCLIVVYP
                     HTSNWDFPLGLLVRFALGWPIGWIGKHTLFRWPVSGLLRRLGGIPVDRGQPGGFVEGM
                     AGAFRSRDHLLVAIAPEGTRRRTAGWKSGFYRIARAADVPVGLGFIDYRRKRLGIAAY
                     VRLTGNVAADMARIREVYAGMCGYRPDQASPVVLPESERL"
     gene            48730..49281
                     /locus_tag="PLH85_03825"
     CDS             48730..49281
                     /locus_tag="PLH85_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63717.1"
                     /translation="MPATAPAFRPPPILDIEASGFGPSSYPIEVGLILPDGCTYCSLI
                     RPLDAWTHWDASAEAIHHVARSTLVAHGRSPADVAHALNLRLHGQTIYCDAWYHDFTW
                     LGRLFDAADLPQLFRLEDIRSLLGETDMEVWHATKTDILAELGLQRHRASNDARVLQQ
                     TLLRVKGWTAPIAAEAQPCDRPV"
     gene            complement(49303..49644)
                     /locus_tag="PLH85_03830"
     CDS             complement(49303..49644)
                     /locus_tag="PLH85_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63718.1"
                     /translation="MTLRTASLLFALLSSTAHACGPYEDDWAGRDKAAHFGVSAALGV
                     ATSQISSDPWTAFALALAPGVAKEVYDSQRACNRFSWKDLSWDAAGALVGVKTGHWLL
                     SPGGVAYRRSF"
     gene            complement(49895..50251)
                     /locus_tag="PLH85_03835"
     CDS             complement(49895..50251)
                     /locus_tag="PLH85_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63719.1"
                     /translation="MNKSLFALSLALALGAAHAADVKPADPRATPADAVKAGAGAAAA
                     GQTAMEQHVRTHAAGKAEAVKRAAVPREAQASAAVRVKDAVAPQGARMDGAKAAVKAE
                     AKPADQAAALAGAVVK"
BASE COUNT         8679 a        18369 c        15711 g         7660 t
ORIGIN      
        1 ccacggtcca gaacgatcaa cgcgtgattg aagtctatct ggggcgctga gatgcaacga
       61 cccccacgct cactttgttc gcggaggggt ggcctagcat ggccaccccg ttcgacaggc
      121 tcggcgcagt gcgccgagaa acccctgtct caccccccca cggcaacgca ggcctccctt
      181 gggacggccc agcgggaggc ctgagatgct gactgtagat tccctcaacc agtattacgg
      241 cggcagccac atcctgcgcg gcctgtcctt caccgccgaa cccggccagg tgaccaccct
      301 gctgggtcgc aacggtgtgg gcaagaccac gctgctcaag agcctgatgg gcctggtgcc
      361 gacccgcagc ggcagcgtca ccttcggcgg gcaggacctc acccacgcgc cgccccacca
      421 gcgcgtgcgc gccggcatcg gctacgtgcc ccaggggcgc gagatcttcc cgcgcctcac
      481 cgtggaggag aacctcctca tgggcctcgc cacgaagccc ggcggcacga agatccccga
      541 gcgcatcttc gacatgttcc cggtgctgcg gcagatgctc caccgccgcg gcggcgacct
      601 ctccggtggc cagcagcagc agctcgccat cggccgcgcc ctcgccatgg ggcccaggct
      661 gctgatcctc gacgagccca ccgaaggcat ccagccgtcg atcatcaagg acatcgagcg
      721 ggccatccgc atgctcgccc agaccggcga gatggcgatc ctgctggtgg aacagtacta
      781 cgacttcgcc gaatccctcg ccgaccagta cctgctgatg gagcggggcg agttcatcct
      841 gcgggggaag ggcgcggaca tgcagaagga cggcgtgcgg gaggccttgt ccctataatc
      901 gcggcgatga aacccgtcga agcgcccctg gcccacggct ggcacgccac cctgtctctc
      961 ggcttcagcc gccgggagac gggcaccgtg ctgtccgaca accgccatcg cggtcccctg
     1021 cgggtgcaga aggccctcta cccggaaggc ccggcggtgt gtcagaccat cctgctgcac
     1081 ccgcccgccg gcattgcggg cggcgatcac ctgcacatcg gcgtcgaggc cggccccggc
     1141 agcatcgccc aactcaccac ccccggcgcc ggcaagtggt atcgctcggc gggccccgag
     1201 gcctcccagc ggctcgattt ccacgtggcc gacggtgccg cgctggaatg gctgccccag
     1261 gagaccatcg tcttcgacgg cgcccgcgcc cgcatggaga cccgcgtgca gctctccggc
     1321 aacgcccgct acctgggctg ggacatcctc tgccccggcc gcgccgccgc cggcgaacgc
     1381 ttccgccagg gccgcatcga cctgcacacg cgcatcgagc gggacggccg gcccctgtgg
     1441 ctggagcgcg gcggcctgct cggcgacgac ccgatgttcg ccagccccgc cggctgggac
     1501 ggtgccggcg tgtgcgccac cctgctcgcc accggcccgg tggacgccga cctcctcgcc
     1561 gcctgccgtg ccgtggcgcc ggccgacggc gcgcgccatg ccgtgaccgt cctgcccggc
     1621 ctgctggtcg cccgctacct gggcgacgcc agcgaagccg cccgccactg gtgcgccgcc
     1681 ctgtgggccg tgctgcgccc tgccctgctc ggccgccccg ccgtgccgcc ccgcatctgg
     1741 aacacctgaa gcccggagat ttccatggaa ctcacccccc gcgagaagga caagctgctg
     1801 atcttcaccg ccggcctgct ggccgagcgg cgcaaggccc gcggcctcaa gctgaactac
     1861 ccggaagccg tcgccctgat cacctgcgcc atcctcgaag gtgcccgcga cgggcgcagc
     1921 gtggccgagc tgatgagcga gggcaccaaa gtgctgaccc gcgccgacgt catggacggc
     1981 gtggccgaga tgatccccga catccaggtg gaagccacct tccccgacgg caccaagctg
     2041 gtgacggtcc atcaccccat tgtgtgaggc gcagacgatg atccccggag aactcctcgc
     2101 cgaacccggc gaactcgaac tcaacgccgg ccgcccgacg atcaccctgg tggtagccaa
     2161 caccggcgac cgcccgatcc aggtcggctc ccactatcac ttttacgaga ccaacgccgg
     2221 cctcagcttc gaccgggaag ccgcccgcgg ttaccgcctc gacatcgccg ccggcaccgc
     2281 cgtgcgcttc gagccgggcc agacgcgcac cgtgcagttg gtcgccctgg cgggtgacag
     2341 gaaggtttat ggcttccgcg gcctcgttca aggagccctg taatggcgaa cacgatttcc
     2401 cgtcaggcct atgccgaaat gttcggcccc accaccggcg accgcctgcg tctggccgat
     2461 accgagctga tcatcgaggt cgagaaggac tacacgctct acggcgagga agtgaaattc
     2521 ggcggcggca aggtgatccg cgacggcatg ggccagagcc agcgcgtgtc gacggaaacc
     2581 gtcgacaccg tgatcaccaa cgcgctgatc gtcgatgccg tcaccggcat cgtcaaggcc
     2641 gacatcggcc tcaaggacgg gcgtatcgcc gccatcggca aggccggcaa cccggacatc
     2701 cagccgggtg tcacgattgt cgtcggcccc ggcaccgagg tcatcgccgg cgaaggcatg
     2761 atcgtcacgg ccggcggcat cgacagccac atccacttca tctgcccgca gcagatcgag
     2821 gaggcgctca attccggcgt caccaccatg atcggcggcg gcaccggccc ggccaccggc
     2881 acgtttgcca ccacctgcac gccggggccg tggcacatcc accgcatgct ggaagcggct
     2941 gacgccttcc cgatgaatct cggcttcctc ggcaagggca acgccagcct gccggaagcg
     3001 ctgcgcgagc aggtcgaagc cggcgtcatc ggtctcaagc tgcacgagga ctggggcacc
     3061 acgccggcag ccatcgactg ctgcctgtcg gtcgccgacg agatggacgt gcaggtcgcc
     3121 atccacaccg acacgctcaa cgaatccggc ttcgtcgagg cgacgattga cgccttcaag
     3181 ggccgcacca tccacacctt ccacaccgaa ggcgccggcg gcggccacgc gccggacatc
     3241 atcaaggcgg ccgggctgcc caacgtgctg cccagctcca ccaacccgac catgccttac
     3301 acggtgaaca ccatcgacga gcatctcgac atgctgatgg tgtgccacca cctggatccg
     3361 gccatcgccg aggacatcgc ttttgccgaa tcgcgcatcc gccgcgagac catcgcagcc
     3421 gaagacatcc tccacgacct gggcgtgttc tcgatgatgt cgtcggattc ccaggccatg
     3481 ggccgcgtcg gcgaggtgat catccgcacc tggcaggccg cccacaagat gaaggtccag
     3541 cgcggcacgc tgcccgagga ctgcacgcgc cacgacaact tccgcgtcaa gcgctacatc
     3601 gccaaataca cgatcaaccc ggccctgacc cacggcatcg cccacaccgt cggctccgtc
     3661 gaagtgggca agctggccga cctggtgctg tggcgaccgg ccttcttcgg tgtcaaaccg
     3721 tcgctgatcc tgaagggcgg catgatcgca gccgccgcca tgggcgaccc gaacgcgtcc
     3781 atcccgacgc cccagcccgt gcattaccgg cccatgttcg gcagcttcgg caaggcgctg
     3841 aagacctcgg tgaccttcgt gtcccaggcc gcgttgaaga accccgccgt tgccgccctg
     3901 ggcctgagca agccgctggt ggccgtctcc ggcacccgca cgctgagcaa ggccgacatg
     3961 gtgcataacg gcgccacgcc cgagatcacc gtcgatcccg aaacctatgt ggtgaaggcc
     4021 gacggtgtgc atctggtgtg cgaaccggcc accgagctgc ccctggcgca gcgttacttc
     4081 ctgttctgaa gccgtccgcg ccatgaccga cccgatcgcc cgcctcgcca acctgggacc
     4141 gaagtcctcg gccatgctgg cggcggcggg catcgccact gtagaggatc tgcgcgccct
     4201 cggcgcggtg gcagcctacg cccgcgtcaa ggcccgcgac ccgcacgcca gcctcaacct
     4261 gctgtgggcc ctcgaaggcg ccctcagtgg cctgccctgg cagaccgtgg cgcgggaaca
     4321 ccgcaccagc ctcctgctgg ccctggaaac ctgtcaaaca tcgcccgccc ccgaatgctg
     4381 aacctcacgc aacgcacgaa cgcccccgcc accgatgccg tggccctcgc ctacgacgac
     4441 cgcaaacgca gccgcctcaa ggtgacgctc gcctccggcc gcgaggccgg catctttctc
     4501 gagcggggcg agcacctcca gggcggcgac cgcctggccg gcgacgacgg cgccaccgtc
     4561 gtggagatcc tcgccgcgcc ggagcagctc atcgaagccg aggccgggac gcccctgctc
     4621 ttcgcccgcg ccgcctatca cctgggcaac cgccacgtgc cggtgcagct cctgcccacc
     4681 gccagcggcg gcgtactgcg cttccagacc gaccacgtgc tggccgagat ggtgaagggc
     4741 ctgggctgta ccgtggccga gaccgacgcc cccttccagc ccgaggccgg cgcttacggc
     4801 gcccacggcg gccatcagca tccctaccat cccggcgagg cgggcgccgc cgggcaggcc
     4861 gacctccacg accccggcca cggcccgcac cgctccgtcc caaagatcca ccagttccgg
     4921 gcccgctgag catgccctac gtcaacgtcc agatcacccg cggcgccacg cgggaacaga
     4981 aggccgagat cgtgcgcgac atcaccgcca ccctggtgcg cgtcctcggc aagcggccgg
     5041 agcacaccca cgtcgtcctc caggagatcg ccgaggagga ctggggctac gccggtctgc
     5101 tcaccgacga gtggaaacgt cagcagggag gcgcctgatg cgcaccggta ccctgctgcg
     5161 tcaaacgccg ctcctcgtcg ccctgctcgc cagttccgcg ccgggtgccg ccgtgacacc
     5221 cgcctaccgc ggtccggacc tcagcggcac ctatgtctgc accggccacg accatcagga
     5281 cggaccctat accggcacgg tgaccctcgc cctgctgcgc acgcgcagca ccggcgccca
     5341 ggccagctac gacttccgcc tcgacgtgcc cggttacggc agctatcccg gccacgccgc
     5401 ggcccagggg cgccacatgg ccatcagctt cgccaacacc gacccgacga cccacgacta
     5461 cggcaccggc atcgcccgct tccaccgcca gcatggcaaa tgggccttcg acaagttcta
     5521 ccatgagccc gaatacaagg gcggcaactt cggcaccgag cactgcgtgc agcgctgaga
     5581 tggaacgccc gcccccctca ctccgttcgc tgtccccctg tgcaggtgca agcctccctt
     5641 cgaacggccc gtcaggcggc ttgagatgaa cgacaccctg gccctcgccc gcctcctgca
     5701 actggccagc ccggccctgc cggtgggcgc ctacacctat tcccagggcc tcgagtgggc
     5761 ggtggaatcc ggcgtcatcc gtgacgaagc cggcgccgcg cgctggatcg gcgatctgct
     5821 ggcccacgcc atcggccgct tcgaggcccc gctgctggcc cgccagatgc aggcctggcg
     5881 cgacaccgat cacaccgaag tcctgcgcct cgacgcggat ttcctggcca gccgtgaaag
     5941 cgccgagctg cgcgccgaga cggcccagat gggcttctcc ctgcgccgcc tgctgcgcga
     6001 cctgcgggac gagggcctgg cgccggtggc gttggtggtc gatcccctgg ccgaagtggc
     6061 ctttcccacg gtgtggagcg gcatcgccgc cacctggggc atcgcgcccc acgcggccgt
     6121 cgtcgcctac ctgtggtcct gggccgaaaa ccaggtgatg gccgccctca aggcggtgcc
     6181 cctggggcag gccgccggcc agcgcctgct cgccactctg ggcacgcgcc tgcccgcttt
     6241 ggctgacgcg gccctcgatt tgcccgaggc cgcctggagc aacttcacgc cggccttcgc
     6301 cctcgcctgc gcccgtcacg aaacccagta ttcccgactc ttcagatcct gaccatgacc
     6361 cagcaagccc tccgcgtcgg catcggtggc cccgtcggct ccggcaagac cgccctcacc
     6421 ctcgccctgt gccaggccct gcgcgagcag atcgacatgg ctgtcgtcac caacgacatc
     6481 tacaccgccg aggacgccca gttcctcgtc cgccacgccg ccctgccgcc cgagcgcatc
     6541 atcggcgtgg agaccggcgg ctgtccccac accgccatcc gcgaggacgc ctccatcaac
     6601 ctggaggccg tggaccgcct ccagcgcgcc ttcccggatc tgcgcctgat cctcgtggag
     6661 tccggcggcg acaatctggc ggccaccttc tcgccggaac tctccgacct caccctctac
     6721 gtcatcgacg tggccgccgg cgagaagatc ccccgcaaag gcgggccggg catcaccaag
     6781 tccgatctgc tggtcatcaa caagatcgat ctggcccctc tggtcggcgc ctccctcgat
     6841 gtgatggccc acgacgcccg cgcccagcgc ggcgaacggc ccttcgtctt caccaacctg
     6901 aagagcggcg aagggcttga catcgtcatc cgcttcatcc gcgaccgcgg catgctcgat
     6961 tgatatcggg ccgccgggcg tgaccgacat cgtccacccg cccggcggcc ccacgagcgc
     7021 ggggaagttc attacaatgg atgactggtc ccgtcatatc cctgtcaatg agcgccagca
     7081 gcccttccct ctccgaacgc tcggccccga ttctggattg gctcgcctcc gagacgtcgg
     7141 aggacaccga tctcgaaatc tccgccctgg tccgtcacct ggaggagatg gccgggtcgg
     7201 aaatcaaccg gaatcagttc taccgctgcc ttgagctgat ccacacccgc agcctcgtga
     7261 tcgccgccca gatgcggcag cacctggcga cgggcgccct gcctctgacc atcggcctgc
     7321 accggtccgc cacacgcatg atcggcgcgc tcggcaagat cgtggacggc tacgagcaac
     7381 tgctcgaaga aacccgcaac cgcctcgtgc gcaccacccg gcgcaacccg gaaacgctca
     7441 gcgccaaggc gctgcgcctg ctcggcgagc agctcaccct ctcccatctg gcgggcagcg
     7501 cagcgccctt cgatttctgg cagcgcgccc acgccctctt ccgcctgtcg ggccgcgatg
     7561 cccagaccgg cgacggcgcc gccgaagcgc cggaaagcgt cgccaccgcc tacaagtacc
     7621 tgctgtgcct cgccgccacc caacccgaag ggctcaccgg acgggaaatc tgctggctgt
     7681 ccgactacat cgccgaacac gccaacgagt gtcgcctgcg ggacacggcg cccactcgca
     7741 accgggcggc atggtactgg atcgacccgg accagaacgc ctcgccggtc tcgttttcgc
     7801 gacgcgaacc gcccccggtg gacggactgc tgtacttctc cgccgaagag ctcgcccgcg
     7861 gggccaccga actgatcgac acccttgacg gtgaccaggc gcccacccac gatctgccgg
     7921 cctggctgcg ccggggcgag gccgccacct tgctgcgccg cgtccgggaa tactgggccg
     7981 cgccgccgca ccgggcccac acgcggcgcc gcaaccagta ccccatcacc gtctgctgcg
     8041 gcctcgaatc catctggaaa atgctgcggg cgccagccgg tccccgccag aaccgcttct
     8101 ccaccgaatg gatggtggtg aacgaaagcc ccaccggcta cgccatcatg caggtcaccg
     8161 gcgccgcggc caatctgggg cctggcatcg cgctggccct gcgccgcagc gaagacgaac
     8221 aatggaccat ctgcgtcgtg cgctggatcc gcaccgagac ccccgagcag gtggagctcg
     8281 gcctgcaggt cgtggccaac tcggccaagc ccgtgaccgt gggtttccgc aacagcgcca
     8341 aggcccagcc gatgaaacct gcactggtgc tgccgccgct gccggccctt ggcaaggccc
     8401 agtccatcct cgcgccgggc ggcacctaca ctgcgcgccg cttcatgctg gtctccgaca
     8461 cccagcgcct ttacgtggcc caggggcgcc tgctgagtct cgacatgcag accgccgccg
     8521 tcgagctgtt ccagtacgag atcgaccctt atccgctctg atccttcgca tcgccggcct
     8581 cggccaggac gctgagcggc agttcgctcg caacattaag gcgcgccacc caggtctccc
     8641 acaggggacg gatctgcgcc tccagcggcg tcgccaccgc gtctttccgc ggcacgccat
     8701 cccacgcaaa cagctgcgcc acctccgcca gactcaccgc atcggccgcc cgcgccagca
     8761 gccagccccc ttcatcgctg cggaccacga tgcgcgcccg ctggcagctt tcaagcaccg
     8821 cccgcacctc gtcgtgaccg gcccgggccg tgcgcgccag atgccctacg tcgggcgtga
     8881 ggccgcgccg ctgggcccgc accagctgcc ccagtaccag cagcgcgaga tacaaggacc
     8941 gccccgggaa cgccggcagc atgcgtccga gcagggtgcg ctcgggcagc acggcagcca
     9001 ccacggcacc gagcaggatc acgatccagg acagatacag ccacatcagg aaaatcggca
     9061 ccacggcaaa cgccccgtac accagcgtat agctggtgaa atgggtcaga tacagcccaa
     9121 acaggcgctg catggagacc agcagcagcg cggccaggaa gcccgccgcg agcgcatcga
     9181 cggggcggac ggtgcagtgg ggaacaatgt aatacacggc ggcgaaaagc ccggtgagca
     9241 gcgccaccgg catcaggcgc agggccactg cccccagcca caccggctcc cccaccagat
     9301 cgagggacag ggacaggacc gcagaggacg caaacacaca acctgccagg aagaccggtc
     9361 caaccgacag ggccgcccag tacaccgcga agcgcgtggc ccagcggcgt gtaccgcgca
     9421 cgttccagat ctggttgatg gcctgatcga tggtcttgat gagcgacagg gccgtcacca
     9481 ccagcaccac cccgccgacg atggtcagat tggtggactt ctggctgaac tgcaccgcat
     9541 aggctgcaat taccttcccc gccttgtccg gcagcaggtt gtccagcagg aagcccttga
     9601 gtgcctcgct gaactgggtc gactggggaa agcggctgaa ggtcagcacc accagggtga
     9661 cgaagggcac cagcgagagc agggacgtga aggtcaggct gcccgccacc tgggcacatc
     9721 gcccctcatg gaaacgccgc cagacggcca cggcgagatg gcgcgcagcc tcggccgtca
     9781 atcggccctc ccgcaaagaa tgggtgtcag tgatcatttc cgtataatgc cctccccacg
     9841 tccgggccac agcatgcacg aaatcctcgt tctctattac agccaccggg gcgccgtgcg
     9901 cgccctggcg gaactcatcg cccgcggtat cgacgccgtg cccggcgtgg ccgcgcgtct
     9961 gcgtaccgtg ccacgcgtct ccacggtctg cgaagccacc gaaggggaca ttcccgaacg
    10021 cggcgccccc tacgtggaga tccgcgacct ggaggaatgc atcgggctcg ccctgggcag
    10081 cccgtcgcgc ttcggcaaca tggcggcgcc gatgaagtat tttctcgacg gcacggccgg
    10141 tccgtggctg tccggcgccc tggcgggcaa gccggcctgt gtattcacct cttccgccag
    10201 cccccacggc gggcaggaaa gcaccctgct gtcgatgatg attcccttgc tccaccacgg
    10261 cctgctgctg gtcggcctgc ccttctccga gcccgacctg acccgcaccc gcacgggtgg
    10321 ctctccctat ggcgcgaccc acgtcgccgg cccggacgac tccaacccca tcagcgacga
    10381 ggaaaaacgc ctggccgtcg ccctcggccg ccgcctcgcc gaaactgccc tcaaactggt
    10441 cgcccgatga aaccgaaaac cctgtccctg atcgccagcc tgacccttgt caccctgatc
    10501 gccctgtgcc tggcctggga aaccgtgctc gcgcccgtgc gtccaggcgg ttcctggatg
    10561 accctgaagg tgctgcccct gatggcggca ctgttcggca tcctgcgcgg cacccgttac
    10621 acctatcagt ggtcgtcgat gatgatcctg ctgtacgtca ccgaaggcgt ggtgcgcgcc
    10681 agcgacccgg cgccgggcgg tcagctggcg ctggccgaag ccgtgctgag catcgtgttc
    10741 ttcgtggccg tggtgctgta cgcacgcaac acggccccgt ctcgcctcgc ccgcgccgct
    10801 cagcaggacg cctgacccgc agcaccgggc ggcggacccg ggcgctgctc agacctgcgg
    10861 attcagccgc tccagcttgg cgtggagctt gttgagggcg ttgaggtagg ccttcgcgga
    10921 ggcgacgatg atgtccgtgt cggcgccctg gccgttcacg atcttgccgt ccttggacag
    10981 acgcacggtc acctcgccct gggcatcggt gccggtggtg atggcgttca ccgagtacag
    11041 caggagctgg gtgccgctgt cggcgatcac ctcgatggcc ttgaaggccg catccaccgg
    11101 cccggagcca caggattcgg agttggcctc cttgccgccc accgacaggg tcagcttcgc
    11161 gcagggcgtc tcgccggttt ccgagtggaa ggatgaggac accagacgga agtgctcctt
    11221 gggctcatag ctttcatcgc tgagcaaggc ctggaggtct tcgtcgtaga tctcgtgctt
    11281 cttgtccgcc agttccttga aacgggcgaa ggcgtggttg agctgctctt ccgagccgat
    11341 gacgatgccc agttcctgca ggcgcgagcg gaaggcgtta cggccggagt gcttgccgag
    11401 caccagcttg ttggcggtcc agcccacgtc ctcggcgcgc atgatctcgt aggtttcgcg
    11461 gtgcttgagc acgccgtcct ggtgaatgcc cgactcgtgg gcaaaagcgt tggcgccaac
    11521 gatggccttg ttgggctgta ccgggaagcc ggtgatgccg gacaccagct tggaagccgg
    11581 cacgatctgg gtggtgtcga tgcggctgtc gcacaggaag atgtccttgc gcgtgcgcac
    11641 ggccatcacc acttcttcca gcgaggcatt gccggcgcgc tcgccgagac cgttgacagt
    11701 gcattccacc tggcgggccc ccgcgcgcac ggcggcaagg gagttggcca ctgccaggcc
    11761 caggtcgttg tggcaatgga ccgaccacac caccttgtcg gaaccgggaa cacgctcgat
    11821 caggctgcgg atcgtcgcgg cgaactgctc gggcacgttg tagccgacgg tgtcaggcac
    11881 gttgatggtg gtcgcgccag cgcggatgac ggcttcgaag atgcggcaca ggaaatcaag
    11941 ctcggagcgg ccggcgtcct cggcggagaa ctcgatgtcg tcggtgtact cccgcgccca
    12001 gccgatggcc ttcacggcct gcgccaccac ctcgtcgggg ctcatgcgca gcttcttttc
    12061 catgtggatc gggctggtgg caatgaaggt gtggatgcgc ccggagcggg ccggacggat
    12121 cgcttcgccg gcgcggcgga tgtcgttctc gttggcgcgg gcgagggagc agacggtgga
    12181 atccttgatc gcctcggcca ccgccttcac cgcctcgaaa tcgccattgg acgccgccgc
    12241 gaaacccgcc tcgatgacgt tgaccttcat ccgttccagc tggcgggcga tgcgcagctt
    12301 ctcctcacgg gtcatggacg cgccgggact ctgctcgcca tcgcgcaagg tcgtgtcgaa
    12361 gatgatcaac tggtcgttca tgctgggctc ctgccgggat tcggactttc agattagccg
    12421 acgtctcggc cacggcggcc gaaaacgttt caatgaaggg gatagtgtgt tcagcgcgcg
    12481 gggcgcagtc gcagatcgcg aaggacgggc ccaccgccac ggacaggcgg tctcccggag
    12541 caagcggaaa tcggaagacg gggaggatgc atcggcggac tataaatcag atttttccgc
    12601 cccggcaacc gctgccttgc gtcggccgag ccagcgccac gcggcaaaca catagccgga
    12661 cgcggcgtag cacacgaaca ggccgaacag cacaccctgg ggatagctcg acaccagggc
    12721 aaagcctgcc acgaaggcga tcaccacgaa gaaaggcacg ctgcggcgca ggttgatctc
    12781 cttgccgctg tagaacagca cgttgctgac catggtgatg ccggcgaaga tcgtcagtgt
    12841 gcaggccacc cagcgcacgt cctgaccgct caggccgtta tcgatgacca cccacaccag
    12901 ccccgccacc agcgccgctg cggccgggct gggcagaccc tggaagtagc gcttgtcgat
    12961 gacgtcgatg ttggtgttga agcgggcaag gcgcagcgca gcgccggcac aatagatgaa
    13021 cgcagcgatc cagcccagct tgcccatgcc cttgagggcc cactcataca cgatgaggga
    13081 cggcgcggcg ccaaaagaca ccatgtctgc cagcgagtcg tattcggcgc cgaaggcgct
    13141 ctgggtgcgt gtgaggcgcg cgacgcggcc gtcgaggccg tccatcacca tggccacgaa
    13201 gatcgccacg gccgcctgtt cgaactggcc gttcatggcc tgcacgatgg catagaagcc
    13261 ggcgaacagg gccgcggtcg tgaacaggtt tggaaggatg tagatccccc gacgacgtct
    13321 ttcggggttc aacaggggct tgcgggcgta cggatcggcc atggagggga cgatgggtga
    13381 ggtcgtggaa ttgtacccga tgcggaccga caggcggaaa gcggaacaac ccgcgcgcct
    13441 catggcggcc cgtcaacgac aaaaggggga ctgtcgtccc ccttttgcag gctgcggatg
    13501 gccaggctca gttcttggac tgatccacca gcttgttggc cttgatccac ggcatcatgt
    13561 cgcgcagctg ggcgcccacc gtttcgatgg gatgcacggc attcagacgg cgacgggccg
    13621 tcatggccgg gtagttggtg cggccttcca ggatgaacat cttggcgtag tcgccggtct
    13681 ggatgcgctt cagggcgttg cgcatggccg cgcgggactg ctcgttgatg acttcggtgc
    13741 cggtcacgta ctcaccgaac tccgcattgt tggagatgga gtagttcatg ttggcgatgc
    13801 cgccttcgta catcaggtcg acgatcagct tcagttcgtg caggcactcg aagtaggcca
    13861 tttccggcgc gtagccggct tccaccagcg tctcgaagcc catcttgacc agctcgacgg
    13921 cgccgccgca cagcacggcc tgctcgccga agaggtcggt ctcggtctct tcgcggaagt
    13981 tggtctcgat cacgccgccc ttggtgccgc cgttggccgc cgcgtaggac agggcgatgt
    14041 cacgggcctt gccggacttg tcctggtaga ccgcgatcag ggacggcacg ccgccaccct
    14101 tgaggtattc ggagcgcacg gtgtggccgg gacccttggg ggcgaccatg atcacgtcca
    14161 gatcggcgcg gggcacgacc tggttgtaat gcacgttgaa gccgtgagcg aaggccagca
    14221 cgccgccctg cttgatgttg ggctcgacgt cgttgcggta cacctcgggg atgttctcat
    14281 ccggcagcag gatcatgacc acgtcggcag cgcggacggc ctcggccact tccgccacct
    14341 tcaggccggc gccttcggcc ttggaccagg acgcgccgcc cttgcgcagg ccgaccgtca
    14401 cgttgacgcc ggagtcgcgc aggttctggg cgtgggcgtg accctgggag ccgtaaccga
    14461 cgatggtgac ctgcttgccc ttgatcaggg agaggtctgc atccttatcg taataaacct
    14521 tcatttcatg cctttctgac acaggataag cgcaggcgcg gaacgcgccg catcacactt
    14581 tcaagacccg gtcgccgcga cccacgccgc agatgccgga acggacggtt tcgagaatca
    14641 gggccggatc gatggcgccg acgaaggaat cgagcttggc ctgggtgccg gtgagttcga
    14701 tgacgtaggt gctctcggta acgtcaatga tgcggccgcg gaagatgtcc gacagacgct
    14761 tcatctcgtc ccggtccttg ccggtggcgc gcaccttgac gagcatcagt tcgcgctcga
    14821 tgtgggccga ttcggacaga tcgaccacct tcaccacctc gatcagcttg ttgagctgct
    14881 tggtgatctg ctcgacgatt tcgtcggagc cgctggtgac gatggtcatg cgcgataccg
    14941 acgcatcctc ggtcggtgcc acggtgaggg attcgatgtt gtagccgcgg gccgaaaaca
    15001 gccccgagac gcgcgacagg gcgcccgatt cgttttcgat gagaagggaa atgacgtgtc
    15061 tcatgtgcgt ggtcgaagaa atgggcagat agcggtgcaa ccaggcgccg ctggcggacc
    15121 ggggcgaaac cggcatcgcc gtgtcgcgcc ccgccgtcac cggcttacag gtcctcggcg
    15181 gacaggatca tgtccgtgag ccccttgccg ccctggacca tggggtagac gttctcggtc
    15241 gggtccacct ggaagtccag gaacaccaga tcgttcttgc gctggaaggc ctcccggagg
    15301 gcgccttcca catcggccgg cttgtccacg cggatgccca catggccgta cgcttcggcc
    15361 agcttgacga agtccggcag ggaatccatg taggactccg aataacggtt gccgtggaag
    15421 atctcctgcc actgacgcac catgcccagg tagcggttgt tgagcatcac caccttgatg
    15481 ggcagacgga actgcttgca ggtggcgagt tcctggatgt tcatctggat cgaggcttct
    15541 ccggtgatgc atgccaccgg cgagccggga tgggccagtt gcacccccat ggcggccggc
    15601 atgccgaagc ccatggtgcc cagaccgccg gaattgatcc agcgccgcgg cttgtcgaac
    15661 ttgtagtact gggcgcccca catctggtgc tggcccacgt cggaggtcac gaaagcgtcg
    15721 ccaccagtgg cttcccacag cttctgcacg acgaactggg gcttgatgat gtcgtcgggc
    15781 tgggtgaact tgaggcaatc gcggcggcgc cattcctcga tctgcttcca ccacacgtcc
    15841 agcgcttcag catccgggcg ctcgctcgtc ccctcgagca gggcgagcag ttcacccagc
    15901 acttcgccca cgtcgccgac gatgggcacg tcaaccttca cgcgcttgga gatggacgac
    15961 ggatcgatgt cgatgtggat gatcttgcgc ggttcggagg cgaaatgatc cggattgccg
    16021 atcacccggt catcgaagcg ggcccccacg gccagcagca cgtcgcagta gtgcatgccc
    16081 atgttggctt cgtaggtgcc gtgcatgccc agcatgccca gacactggcg gtcggtggcg
    16141 ggatagccgc ccagacccat cagcgtgttg gtgcagggga aaccgagcct gcgaacgagg
    16201 ttcgtcagct tctcggaagc atcgccgagc accacgccgc cgcccgcgta gaccatcggg
    16261 cgcttggctt ccagcaggag ttgaagcgcc ttgcggatct gaccgtgatg gcccttgacc
    16321 accgggttgt aggaccgcat cgacacgctc gccggataga cgaactcgca cttctgggcg
    16381 gtgatgtcct tggggatatc caccagcacc ggcccgggac ggccggtacg agccagatag
    16441 aaggctttct tgagcgtggg cgcgatctcg cgcacgtccc gaaccaggaa attgtgcttc
    16501 acacagggcc gggtgatgcc gaccgtatcg acttcctgga aggcatcctg gccgatcgcc
    16561 gcggaaggca cctgcccgga gatgaccacc atcggaatgg agtcgcaata ggcggtcgca
    16621 atgccggtca ccgcattggt cgcaccgggc ccggaggtca ccagcgccac ccccacccga
    16681 tcagtggaac gggcatagcc gtcggcggca tggatggcgg cctgttcatg gcggaccagc
    16741 acgtgacgaa ccttgtcctg cttgaagatt tcgtcgtaga tgtagagcac ggcgccaccg
    16801 ggatagccga acagatagtc gaccttttct tcctggagac acctgattac aatttccgca
    16861 ccggtcaata ccattgctgc acccgaagct gtctcagcca tgtgaaacgt tgaaccatac
    16921 cgccgacccg gcgtttggtc aaggtgacgt ttctgtggtg gtccgataca gtggcgggtg
    16981 ctttgtcgtt tgagcggcta cagctcggct tcgcccgcga ggaactcgcc cctgtcttcc
    17041 cgaatcgaac tctccgactt tctcgccagc gtcgagaagc gcgccttcaa gcaggcgctc
    17101 tttgcggtgc gcaacgagga gaccgccctc gacatcgtcc aggacgcgat gatgaaactc
    17161 gcggagaagt acggcgaccg tccacccgaa gagttgccga tgctcttcca gcgcatcctg
    17221 cagaacgtca tccgcgacca ctaccggcgg cagaaggtcc gcagcatgtg gaccaccctg
    17281 ctgtccgcgt tcagcggccc ggacgaggag gatgagcacg acccgctgga gaccctggaa
    17341 tccgccgaca gcgagttccg cagcccgtcc ccccaggcgg aggttgaaca gcaacagacc
    17401 ctgtccctga tcgaacggga aatcgaaaag cttccgccgc gtcaacgcga agcgtttctg
    17461 atgcgttact gggaagaaat ggacatcgcg gaaacggccg ccgccatggg ctgttcggag
    17521 ggcagtgtca agacccactg ctcccgggcc actcatgcat tggccgcagc ccttgcagca
    17581 aaaggcatta ccttatgatc gagcacgaaa tccaccggcg tctgcgtcaa catctcaacc
    17641 gctcgctgca gcgcatcgaa ccggacacgt cagagcgcct gcacgccgcc cgacgcaagg
    17701 ccctgtcacg gcaaaaggtg gctgtccacg gcctcagtct ggctggcgcg gggcgtctga
    17761 tcaccgacca cctgccagac cggcggactg ccgtcgccat ggtgctggtt gtcgtcattg
    17821 ccctggccag cggcttcctc ggcgacctgc aacgcacggc cgacatcgaa gaggtggaca
    17881 gctcgctgct cgccgatgac ctccccatcg acgcctacct ggaccgcggt ttcgacacat
    17941 gggtgcaaag ccaagctctc gagtaattgc tgcgctggcc ctgtgctggg ccgcccacgc
    18001 tgccgccgtc ccgccgctca aggccccttc ctggaacgaa ctcacgccgg cccagcagcg
    18061 cacccttgcg ccgctcgtca aggaatggag caacctcccg gacgaccgca agcgcaagtg
    18121 ggtcggcatc gctgaccgct atcccggcct cacacccgaa gagcaggcca ggcttcagca
    18181 gcgcatgcat gaatgggcct ccctcacccc tgcccagcgg gagtctgcgc gggagcagta
    18241 ccgcaacctg caaaagatgc cgcccgaaca gcgtcaggcc ttgcgggaaa agtgggaagc
    18301 ctatcttgag ctgccggagg aggagaagaa gcgtttcacc gacgccgcgc ccaagcccgg
    18361 ccccaaaccg cctgtacccg gacagccggc cacaccgatc aagcccctga ctgcaccgcc
    18421 gcagccggcg cgcccggtcg ttctacccaa gccggtaccg ccgcccgcgc ccctcacggc
    18481 ccccgctcca gtgcctgttc ctgtgcccgc ccaggcgccc gtcgtcgccg gccaggatcc
    18541 caccccaccc aaaccctgat ccatgatgcc ttccaccgcc acgtccggag cgacgccccg
    18601 gccgacgggt gagctcgcca ccctgcgccg acgcctcgcc agcatgctct acgaagtcct
    18661 gctcctgctc ggcgtacttg ccctcacctt cctcgtgccg ctgctgatcc ttgggattgg
    18721 cttcggcatc ctgccacccg gcggtgtcat gtggttgtgg atctatctgg cgctgggcgg
    18781 atatttcgtg tggtactggc gtcagggcgg ccagacgctg gccatgcaga catggaagct
    18841 gcgcatcgtt gcggcgaacg gcgacaccgt atcggtccgc caggcctggc tgcggtactc
    18901 gctggcctgg gcctcgctgt gcgtcggcgg cctcggcctg ttctgggccc tgatcgaccc
    18961 ggagcgtcag ttcctgcacg accggctggc cggcacgcgc atcgtcctgc tgcttgcccc
    19021 accggctgac tgaagacgcc tcagcgccgc tcgacccacc acatcatcgt cgccgccgcg
    19081 acaaggaagg tgacgctagg ggtcagcgcc gcgaccgccg gcacccagcc gttgatcaca
    19141 ccgaggttgg agaacagacc gttgagcagg tggaagccga cgccgatcat gatccccgag
    19201 aagaccttca ggctcacccc gcccatgcgg ttctgcatgt aggcgaaggg cagcgcgagc
    19261 cccatcatca cgagggccgc gaagggatag acgagcttct tccacagggc gatctcgtag
    19321 cgggtggtgc gctggttgtt gtcttccaga tggcggatgt aggaataaag cgtcgtcacg
    19381 gacatccgct ccggcaccac catcagcacc gacagcacgt ccggcgtgag ttcggaatgc
    19441 cacaccagct cgggcaggga gaccgtttcg gtgcggtcgc cgaggaagcg ggtttgcgcc
    19501 acctcggtca ggcgccagcc attgcgctcg tcgaacacgc cccgcgtggc ctcgctgatg
    19561 gacaccaggg catggcggtc atcgaactca tagagccgga caccttccag cgtggtgtcg
    19621 ggcatcacgt tgcggacatt gacgaaacga tgaccgtccc gcacccacag gccggtgcgc
    19681 agctcctggg acaccatgga gcgggtcgcg gccagacgcc attgctgggc ggcccgttcg
    19741 gcgggcggcg ccaggtattc gccaaatacg aaggtgatcg ccacgaagac gctcccgatg
    19801 gtcaggagcg ccgccaggaa gcgcccggtc gataggccgg atgcccgcaa caccgtgatc
    19861 tcggaatgcc gcgcgagggt ggtgagcgca tacagcgtac cgatcagcac cgcgatcggc
    19921 atcagctcgt agacccggcc cggcaggatc atcgccacgt acagggccgc gtggtagagc
    19981 atgtaaccgt tcttgcccac gtcgtcgagt tcattgacca gatcgaagaa ggcgaacaac
    20041 ccgaggaagg ccagcagcac catggccacg gcggcgtaga tttccctggc cagataacgg
    20101 acgaagatcg gggccatcag cgccgcctcc atcgcgcgaa cacgctcagg cgcttccaga
    20161 acagcaccgc cagcaacagc atcatcacca catggggtgc catcagcgcc acctcgaagc
    20221 ccagccgacc ctgcgccacc caggcctgac tcacgctgag ggcgttgcta tagatcagat
    20281 acaccaggat cgccaggatc aggttgttgg tccgtcccgc gcggggattg acaaaggaca
    20341 ggggaatcgc cagcagcgcc agcatcacgg ccagcacggg cgtactcagc cgggccagca
    20401 gttccccccg gtacaggttg cccttttcct tgagcaaggc cgggaaggtc agggtcttgg
    20461 gcagacgcgg gcccgtcacc gcctccttgg actgcatgcg gatctggtag cgctcgaact
    20521 ccatcacccg gtagctcggg gtgcccggca cgccttcgta gcgccgaccg tgttcgagga
    20581 tgacgaagcg gttgcccagg ccatccacct cggaatgacc gttggccgcg accatcacgc
    20641 cgagtcggcc gttctgcatg gaactggcga agacgttgcg cacgcgccct tcctcacccg
    20701 aggcggcctc cacgaagaac acccgctcgc cgccggagga ttcgcggaac atgcccggcg
    20761 ccacccgcga cacgtcgtcc cggttgttga gttgctcctt gtattcaacg cttttcttct
    20821 gggcccaggg cgccccgaag agagacagca gggccaccac aatcaccacc ggcagcgcga
    20881 agcgcaacac cggccggacc catgcggtga gcggcaggcc gcaggagaac cacaccacca
    20941 tctcggaatc ccggtaggcc cgcgagaggg acatcaggat cgcgatgaag accgtgaggg
    21001 acagcagcac cggcatctgg gcgatggcgc cgaaaccgat cagggccagc accgcatcgg
    21061 ccggcacgcg gccacccgcg gcctggccca gcaggcggat cagcacggtg gagatgagga
    21121 tcgcgaacag cgccacgaag accgccacgg cgttctgggt gaattcgcgc tgggcggctc
    21181 ggtgaaagat catgcaggcg gcggaagggg tgcgggtacg gaacggcggg ggacggcggg
    21241 cgaaccgctt tgacgtcggc cggggtgctg cccataatgg cgcgatgtcc gattgacgcg
    21301 tgtttccagg gagaagcggg tggaatttac cataaaggcc gggtcgccgg aaaaactcaa
    21361 aagcggctgc gtggtggccg gcgtgtttgc cggcggcgaa ctgagcgcag ccgccaagac
    21421 cctcgacaag gcctccagcg gcgccctcgc gaccatcctc gcgcggggcg atctggacga
    21481 caaggccggc gccaccctga tgctccacgg cgtgccgggc attgaagcgc cccgcgtgct
    21541 gctggtgagc ctgggcgctg ccagcgaact gaccgacaag cgctaccgtg acgctgtcgc
    21601 cgccgccgcc cggcagctcg ccgcctccat ggccggcgac gcggccttct gcctcgccga
    21661 ggcggacgtg cccggacgcg acctggcctg gcgcatcgcc caggctgcgc agatcctcca
    21721 ggacagcacc taccgctgcg acgagctgaa gtcgcgcaag gaggactcga agaagggcgt
    21781 tgttcacgtg gccttcatca cccgtgccaa gcacggcgac gaactccagg ccgccctccg
    21841 ccgcggcgaa gccatcgccg aaggcctcgc cctcgccaag accctcggca acctgccggg
    21901 caacgtgtgc acgccgggct acctggccac caccgccaag aagctcgcca aggaacacaa
    21961 gatcaaggtc gagatcctcg accgggacgc catggaaaag ctcggcatgg gcgcgctcct
    22021 ctccgtggcc cgcggctccc atgaggcgcc ccgcttcatc atcctccagt acaagggcgg
    22081 caaggccaag gacaagcccg tcgtgctggt gggcaagggc atcaccttcg acaccggcgg
    22141 catctccctc aagccgggcg aaggcatgga cgagatgaag tacgacatgt gcggcgccgc
    22201 cagcgtgctg ggcaccttca aggccatcgc ccgcatggag ctgcccctca acgtgatcgg
    22261 tctgatccca tccaccgaga acatgcccgg cggctccgcc accaagcccg gtgacatcgt
    22321 cacctccatg tccggccaga ccatcgaggt gctcaacacc gacgccgagg gccgcctgat
    22381 cctctgcgac gccctcacct acgccgagcg ctacgaaccc gccgccgtgg tggacatcgc
    22441 caccctcacc ggcgcgtgca tcatcgccct gggcaacctg acctccggcc tcctcgccaa
    22501 cgacgacgcc ctcgccgccg aactgctcgc cgccggccag tctgccggcg acaaggcctg
    22561 gcagctgccc ctgtgggacg aataccagga catgctcaag agcaacttcg ccgacattcc
    22621 caacatcggc agcaagggcg ccggcaccat caccgcggcc tgtttcctgg cgcgcttcac
    22681 caaggcctac acatgggccc acctggacat cgccggcacc gcctggaagt ccggtgccga
    22741 caagggcgcc accggccgcc cggtgccgct gctggtggaa ttcctggccc gtcgcgcagg
    22801 cgcctgaacg gggcgccgcc aatgcccgag atccgcttct accacaacgc gccggaccgc
    22861 cttcgggtgg cctgctccgt caccgccaag gcctggggcc agggtcggcg gatctccgtg
    22921 ctggcgacgg acggcgccca ggcgcgccac ttcgaccagc tcctgtggtc cttccagccc
    22981 ctcgccttcg tgccccacgt gggcgcggaa tcccccctgg cggccgagac gccggtggtg
    23041 atcggcacgc acctggacgc cctgcatcac gaggacgtgc tgatcaacct gggcggcgaa
    23101 ctgccgccgg gtttcgaacg cttcgggctg gtcgtcgaga tcgtcggcca ggacgaattc
    23161 gaacgccacg ccgcgcgtca gcgcttccgc gcctacaagc aggccggatt tcctctccac
    23221 gcccacgatc tcgcccagcg gagcggcgca tgagcgattc ccaccgcccg gtcctggaag
    23281 tccctctcga agacgaggac ggcgaacacc tcctcgccaa ggccgacgcc ctcatgcgcc
    23341 gccacggaca cttcgcggcg cctgccgaac cggacgatct gccggtcctg accgacgtcg
    23401 tcgactatcc cggcgaggaa gcgccgcccg acgacagccc acccgacatc acgccggtcg
    23461 cgctccccaa cgcccgagac gaccagacgg cgcgcatcgt cgacctggaa gccgccatca
    23521 cccgcagcat cgacgcctgg gtctccaccg aactgcccca gatcatgtcc cgcgagttcg
    23581 acgcctttgc cgaacgcctg cgcgaggccg cccgcgaaca catgcgcacc accctgctgc
    23641 ccaccctcac cgcgcagatc gaaaaaaccc tcgacaccga ggaataagcc ccgcccgcgc
    23701 cgccgggcgc cgggagcgca tccgctataa tccgtccttt gccttcacaa gacggacccc
    23761 atggaactgg ccaagagttt cgaaccggcg gacatcgaac gccgctggta tcagcactgg
    23821 gaatcgagcg gctacttcgc cgccggcctc gaccgcagca agcccgccga ccaatccttc
    23881 tgcatcctgc tgccgccgcc caacgtcacc ggcaccctgc acatggggca cggcttcaac
    23941 cagaccctca tggatgccct cacccgctac caccgcatgc ggggctggaa caccctgtgg
    24001 cagccgggca ccgaccacgc cggcatcgcc acccagatcg tcgtggaacg tcaactcgac
    24061 gcccagggcg tcagccgcca cgacctgggc cgcgagaagt tcctggagaa ggtctgggaa
    24121 tggaaggact actcgggcgg caccatcacc cgtcagatgc gccgcctggg caccagcccc
    24181 gactggagcc gcgagcgctt caccatggac gccggcctca acaagatcgt caccgagacc
    24241 ttcgtccgcc tctaccggga aggcctgatc taccggggca agcgtctggt caattgggat
    24301 cccaagctgc acaccgcggt ctccgacctg gaggtggtgc aggaagagga agacggcttc
    24361 ctgtggcacc tcaactaccc gctggccgac ggcagcggtc atctcacggt ggccaccacg
    24421 cggcctgaga ccatgctcgg cgacaccgcc gtgatggtgc atccggatga cgaacgctac
    24481 gcccacctga tcggcaagac cgtccgcctg ccgctggtgg agcgggacat tccgatcatc
    24541 gccgacgcct acgtggatcg cgagttcggc accggcgtgg tcaaggtgac ccccgcccac
    24601 gacttcaacg actacgccgt cggcctgcgc cacggtctgc cgatgatcat cgtgctgact
    24661 ctcgaaggcg ccatccccgc caccggcacg gtggtcgaag gcggtgtcgc cacgggcagc
    24721 gcgcccctgc cggcggacct ggtcgggctc gaccgcttcg acgcccgcaa ggcggtggtc
    24781 gagcgcttcg aagccctcgg cctgctcggc gaggtcaagc cccacaagct ccaggtgccc
    24841 cgcggcgacc gcaccaacgc ggtcatcgag cccatgctca ccgaccagtg gttcgtcgcc
    24901 atgagcaaag ccggcgccga cggcaggtcc atcaccgaaa aggccctgga ggtcgtcgcc
    24961 tccggcgaga tcaagttcgt ccccgaaaac tgggtcaaca cctacaacca gtggctcaac
    25021 aacatccagg actggtgcat ctcccgccag ctctggtggg gccaccagat tcctgcctgg
    25081 tacggcgagg acggccagac cttcgtcgcc cacgacgaag ccgaagcccg cgcccacgcc
    25141 acggcggccg gctacagcgg ccccctcagc cgtgacgagg acgtcctcga cacctggtac
    25201 tcctccgccc tgtggccctt ctcgacgctg gactggacgc ccgactaccc ggcccggtcg
    25261 aacgacgcgc tggatctcta cctgccgtct acggtgctcg tcaccggctt cgacatcatc
    25321 ttcttctggg tggcgcgcat ggtcatgatg accacccaca tcaccggcaa gatccccttc
    25381 cgcgacgtgt acgtgcatgg cctgatccgc gatggcgaag gccagaagat gtccaagtcc
    25441 aagggcaacg tgctcgatcc catcgacctc atcgacggca tcggcgtgga cgacctggtg
    25501 gccaagcgca ccaccggcct gatgaacccc agacaggccg ccagcatcga aaagaagacc
    25561 cgcaaggaat tccccaacgg catccccgcc ttcggcaccg acgccctgcg cttcaccttc
    25621 gccagcctgg ccagccccgg ccgcgacatc aagttcgaca tgaaccgctg cgagggctac
    25681 cgcaacttct gcaacaagct gtggaacgcc acccgcttcg tgctgatgaa cgtccagggc
    25741 cacgacctgg gcctggaaca ccagcagaac ggccccagct gcggcggtaa cggcccgctg
    25801 gccttctcct tcgccgaccg ctggatcgtc agccgcctgc agcgcgtcga gcaggacgtg
    25861 gccctgcact ttgccgaata ccgcttcgac ctggtctcca aggcgatcta cgagttcgtc
    25921 tgggacgagt actgcgactg gtatctggag ctcgccaagg tgcagatcaa caccggcgac
    25981 gaggccagcc agcgtggcac ccgccgcacc ctgatccgcg ttctcgaaac cgtcctgcgc
    26041 ctcgcccacc cgctgatccc ctttgtcacc gaagaactgt ggcagaccgt cgcgcccctg
    26101 gccggccgca aggacaccga gagcatctgc ctcgcccgct accccgaagc tgacctgggc
    26161 cgctgcgacg agaccagtga aggcgacgtg gccgagctca aggccctgat ccatgcctgc
    26221 cgcaacctgc gcggcgagat gaacatctcc ccggcccagc gcatgcccct ggtggcctgc
    26281 ggcaacgcca cccgcctgac gcgcttcgcg ccctacatcg ccggcctggc caagctctcc
    26341 gaagtgcagg tggtggacga gatcggcagc gacgagctcg ctccggtggc cgtggtgggc
    26401 gagcagcgtc tgatgctcaa ggtggaaatc gacatcgccg cagagcggga gcgcctgggc
    26461 aaggagatcg cccgtctcga aggcgagatc gccaaggcca aggccaagct cgccaacgaa
    26521 agcttcgtgg cccgcgcacc ggcggcggta gtggcccagg agcaggagcg cctcgcggcc
    26581 ttcggcgcca cgctggacac actgcggccc cagctcgcca agctcagcgc cgcctgatcg
    26641 ggcacggtcg gcgcccccgc cggctgcccc atccggcact ccaatggaaa acgcccgctt
    26701 gcgcgggcgt tttcgttggc ggtgagcctg ggacaagcga tcagaccgac atggccttca
    26761 cgtgctggat cacctcgccg acggcctgct gggccgaacc gtagaggaga cgacagttgt
    26821 ccttgtagaa cagggcgttc tcgatgcccg agtagcccgt gcccttgccg cgcttgacga
    26881 cgatcacgtt ctgcgccaga tccgcgttga ggatgggcat gccgtagatc gggctggact
    26941 tgtccgtgcg ggcggagggg ttcaccacgt cgttggcgcc gatcaccagg gccacgtcgg
    27001 cctgggcgaa gtcggcgttg atctcttcca ggtcgaagat cttgtcgtag ggcaccccgg
    27061 cttccgccag cagcacgttc atgtggcccg gcatgcggcc cgccaccggg tggatggcga
    27121 agctgacttc cacgccggcc tcttccagca gttcggtcat ctcccagacc ttgtgctggg
    27181 cgccagccac cgccatgccg tagcccggca cgatgatgac cttgctggcg tagcgcatca
    27241 tcgccgccgc gtccatggcg ccgacttcct tctgctcgcc ttccaccgcc tcgccaccgc
    27301 cgctggccgc catcggcacg aagaggatgt tgctgatggg ccggttcatc gccttggcca
    27361 tcagctgggt gagcagcgta ccggccgcgc cgacgacgat gccggcgatg atcagcgccg
    27421 gaatgcccat cacgaagccc tcgaagccca cggccagacc ggtgaaggcg ttgaacaggg
    27481 agatcaccac cggcatgtcc gcgccaccga tgggcagcgt gatgatggtg ccgaggatca
    27541 gcgccaggat gaagaacgcc cccaccagcg tcggatcgac cgccggcgcg atcacgatgg
    27601 ccgcccccag acccacggtg agcagcgcca gcagcacgtt caccgcgttc tgggcgggca
    27661 ggcggaaggc cttcttcatg acgccctgga gcttggccca ggccaccacg gagccggcga
    27721 aggacaccgc accgatcagc gcccccagcg tggcgagcag cgtgaccacg gcgccgtgca
    27781 tctcgccgcg ggcaaattcc accgccgcga tggccgccgc ggcgccaccg cccatgccgt
    27841 tgtagatcgc caccatctgc ggcatgtcgg tcatggcgac cttcttgccg gagatccagg
    27901 ccagcgcccc acccacggcg atcgcgatca ccatcaggag gtagttctgc atgcccggcg
    27961 tggcaaaggt gaccacggtg gccagcacca tgcccacgcc tgcccagacg atgcccttgc
    28021 gcgccgtcac cggcgagctc atgcccttca aaccgaggat gaagagcacc gcggccacga
    28081 agtagctcgc gtcaatcagc cacttgatcg ccatcagttc ttctccttcg acttgaacat
    28141 ggccagcatg cgctcggtca ccacgtaacc gcccgccgcg ttggccgcgc ccagcagcac
    28201 ggcgacgaag ccgatggcct gctcgagggg cgtctgggcc tggccgaggg cgatcatgcc
    28261 gcccaccagc accacgccgt gcacgaagtt ggaacccgac atcagcggcg tatgcaggat
    28321 caccggcacg cgggaaatga cctcgtagcc cgtgaaggcc gcgagcatga agatatacag
    28381 tgcgacaaat ccgtccatgg tcggctcccg cttacaggcc gagggcctgc ttgacgcccg
    28441 cgtggcgaag ctcgcccgcg tgggtcagac aggcaccggc catcacctcg tcttcccagt
    28501 ccagggccag ggccccgtcc ttgatgaagg gcgagatgaa gttgaaaagg ttcttggcgt
    28561 acatctcgga ggcatgcacg ggcatgcggc tcgggatgtg ggtgggaccg atgatgagca
    28621 cttcgcccac ccaggtcttc tcaccaggaa ccgttccggc cacgttgccg ccggtttcgg
    28681 cagccatgtc caccaccacc gaaccgggct tcatgcgcgc caccatctcg gcggagacga
    28741 tcaccggggc tttcttgccc ggaatcgctg ccgtggtgat cagcgcatca cactcgccga
    28801 tggccttgcc gagcttctcg gcctgctgct tcttttcctc gtcggtcagc tcgcgggcat
    28861 agccgccgct gccggacgcg gacacgccgg tatccacgaa cttggccccc agagactcga
    28921 tctgctcctt ggtttccggc cgcacgtcgt agcccgagac catggcgccg aggcgcttgg
    28981 cggtggcgat ggcctgcaga ccggccacgc cggcaccgat gaccagcacc ttggcaggac
    29041 ggatggtacc ggccgcatac gtaagcatcg ggaagaactt gggacagtgg tccgccgcga
    29101 tcagcgcaca ctcgtagccc gccacggccg cctggctcga cagcacgtcc atgctctggg
    29161 cacggctgat gcgaggcagg agctccaggg cgaaacaggt gatgttgcgt tcccgcagca
    29221 ccgcgatgcg ctccgcgctg gaccaaggct gttgaagccc caccagcacg ctgcccggcc
    29281 gcagttcgcg cagcgcgtcc agccccaggg gctggacctg cagcacgacg tcggccttgc
    29341 gcagcaccgc ggcggcatcg tccgccacgt ccgagccggc atagtcgctg ttccggaagt
    29401 aggccggctc gcctgctccc gactcgagca agaggcttgc accgagtccc tgatactttt
    29461 tggccacgtc cggaacgagc gcaacgcgac gctcgcccgg cgtgaactct ttggtcacac
    29521 cgatgaccag aggcatggaa cctccttata gtaggtagtt ggaattctgc tatcgtcccg
    29581 cgagggagtc ggaacgacag agtttgtggg gaatgtaaca atccggagca gcttccggac
    29641 ggcgcgatca ccctctgaca cagattccgg tgatttttag ccggaatccg ggcaaaacat
    29701 gaagaaacct agcaagaaac gaacccgtcc cctccgaaaa gtgggccttc cgccgggaac
    29761 gctcgtccat atcggcgaag tgaagaccga aaaacccagc atcaccctgc tggagtacga
    29821 ttctaccggc atatctgaaa tccgcttcga ttccatcgag gaatcccgca atttcacccg
    29881 cagccgggaa aaaatctggc tcaacgtcta tggcctccac gacccggcca tcatggccga
    29941 gatcggacgc cgcttccgcc tgcacccgct gatccaggaa gacatcctca acacgcagca
    30001 gcggcccaag gtggacgaat ttgccgaata cctctacctc gttgcccgtt acttcgacta
    30061 cgacgccccc gcgcgctgtc tgacatccga ccagatcagc atcgtgctcg ggcgcaactt
    30121 cgtcctgacc ttccaggaac gcgccactgg cgcctttgaa cccgtccgcg aacgtctgcg
    30181 tgctgagacc ggcgccatgc gcgcccacgg tcccgactac ctcgcctacg ccctgatcga
    30241 cacgctggtc gaccgctact tcatcatgct cgaccatctc agcgatctgg ccgaggagct
    30301 ggaggaaagc gccctcaccc ggcccacgcc ttcactgctg gcagagatca accgggtcaa
    30361 gcacgaggcc cgcagcatgc gccgggccgt gtggcccctg cgcgaagtgc tcaacaccct
    30421 gtcccgggcg gacaacgact tcttcaccgc cgacacgcgc ctctacctgc gcgacatcta
    30481 cgaccacacc gtgcacgtcc tcgaatccct cgaatccgtg cgtgacctgc tggccgacct
    30541 gctcgacatt tacctgtcca gcgtgagcaa ccgcctcaac accgaagtcc gcatcctcac
    30601 cgtgctgacc accctgttca tgcccgccac gctgatcgcc ggcatcttcg gcatgaactt
    30661 ccatgaaatg ccgctcctcg ccgatgcgga cggattctgg gtggccatgg gactgatggg
    30721 cggcgcggca gccttcatgg ccgccagctt ctggcgccgg aactggctga agacgcagta
    30781 gaacgcaaca gacgcgggac gggtcagctc tcccgcggag gaacggtctt gggcggaagc
    30841 tccacgagct tgacgatggc gagggagatg ttgtcgccgc ccccccgtgc ccgatgccgg
    30901 gccatttcaa tcagcgtgga cgccgcatcc cgcggcgtat gggacgccag cacgtccccg
    30961 aactcgtctt cggaaatata gccccacaac ccgtcggagc agagcaggaa ggcgtccccc
    31021 ccctgcaggg gtgacacatg cccgagatcg accctcggct cccgatcggc ccccaggcag
    31081 gacagcagca cgttgcgatt gggatggcgc gccgcacttt cggcgtccag ccgccccttg
    31141 cgcaccaact cctccacgag ggaatggtcc gcgctgcggg tcatcagctt gccgtttcgg
    31201 aaatgataaa aacgggaatc cccgcagtgc gcccaatcgg cccggtccgg ctgcatcatg
    31261 aacaccacgg ccgtgctgtg gggatcctgc tcgctggtga agcgggtcag cttgatgacg
    31321 atatgggcct cgtccacgac gctgcgcagg agatcctcgg ggctttccga ggcgggcgcg
    31381 aaggtttcga aattctgctt ggccttcaga acgacctgct cggccgccat ggcgccgccc
    31441 gaatgcccgc ccatgccgtc ggcgaggacg gccagcaagg tacccttgcg ggcgggatgg
    31501 gcaaagacgg ccacccgatc ctgctgctcg gtgcgatccc ccacgtgctt ggccacacag
    31561 gcttccgcct ggtaacgctt cttggccgtg accgtgtcgg gggcaggaat ggaagccagc
    31621 gactgttcga gcatcttatg acaatgaaaa cctgatctga cgacgaccga ccctgcttgc
    31681 gcatcaagcc ttcgcgctat ggagccctca tcacgccatt ttcataaatc cttgcgtaaa
    31741 gaggaaatcc gagacggcaa atgccggatc gttttacaag tttaaccttt ttgcagttgc
    31801 atgctgaata cacgctatat cttctgacgt agggccgtga aatattcatc gatcagctcg
    31861 atccaatgct tcacggggag tcgtcccccg gcggcaagat gctcgatgca accgatgttg
    31921 gcggagacca ccgtcgcacc gccactgtcc accagggcgt ccagcttgtc cgcgcgcagg
    31981 cgtccggcga gcgtcggctg aagcagcgag tatgtgcctg ctgagccaca gcaaagatgg
    32041 gcatcccgca cgggcgccaa ctcaaatccg gccgcctcca gcaacttttc cacgacaccg
    32101 cggatcttct ggccgtgctg caagctgcac ggcgcgtgaa aggcaatgcg gcgccgctcc
    32161 accgcggccg ccgacccggc gatcagggtt tccagcgcat ccccttcggc cgccaacacc
    32221 tcggacagat cgcggcacag ggccgcaatg cgtaccgcct tctctgcata cgccgggtcg
    32281 tgttgcaaca ggtgcccgta atccttgacc tgggtgccgc aaccggacgc ggtcatcacg
    32341 atggcctcga cgcggccggc ggccacgccc ggccaccagg cgtcgatgtt gcgccgggct
    32401 tccatgcggg cgccctcctg atcgtggagg tgaaatttca ccgccccgca gcaccccgct
    32461 cccgcctgct ccacgagcga gacacccagc cggtcgagca cccgggccgc cgccgcattg
    32521 atggcgggcc gcaggctcgg ctgcacgcag cccgccagga cgatcatgcg ccgcggatga
    32581 cgcgcggcgg gccattcgcc cgccgggtcg gctgcgctca ccttgtccgc caaaagccgc
    32641 ggcagcagcg ggcgggcccg tcgcccgagc cccagcgcca gaccgaagag gccggtgttc
    32701 ggcaccagct gccgcaaggc ctcgcggcca agccggtcta cgccctgacg cggcacctga
    32761 cgttccacca ccagccgccc gatgtctgcc aggcgcccgt attcgacacc cgacgggcag
    32821 gtcgtctcgc aggcacggca cgtcaggcag cgatccaggt gcagtcgggt ccgcgccgtg
    32881 gcgggctggc cttccagcac ctgcttgatg aggtagatcc gcccgcgcgg accgtccagt
    32941 tcgtcaccga gcagctggta ggtcgggcag gtggccgtgc agaagccgca atgcacgcac
    33001 ttgcgcagga tcgcctccgc ctcgcggcct tcgggcgtat cccggatgaa atcggcgagt
    33061 tgcgtctgca tggctcacat ccctccgtac aggcggcccg gtccaaacac gcccgccgga
    33121 tcgaaggtct gcttcagccg acgctgcagg gccgccatgg caggcagcag cggatgaaag
    33181 acttcgcccg cccgatcgcc gtgacggaac agggtcgcgt ggccgcccac tgctgcgacc
    33241 cgcgcgcgga gctccgccgc cgattgctcg cccttcagcc agcgctgggc gccgccccat
    33301 tccaccaacg tctcgccggg cacgtccagc ggcggcgcgt cgctcggcac ggacaggcgc
    33361 cacaggggcc ggtcatccgt gaagaaatcg tcggtatgat ccctcagcgc ggcccacagg
    33421 ccatgtgccg ccgtgtccgc cagaacctcg ccgcccagac gccgtatcgc ggcctcgacg
    33481 gccgccgccg cgccagacag gcgtacgtgg agccggcccg cccgccacgc cgtggccgat
    33541 acgggcagag gctgtccgct ccaggcgttc acccgctcca gtgccgccgg ctcgtccagc
    33601 gcaaaggcaa gcgtcgcctc cgccagcggc cgcggcagca ccttgaggga cacctccagg
    33661 agcagcccga gggtgcccag actgcccgcc atcacccgtg cgacgtcgta gccggccaca
    33721 ttcttcatca cctggccacc aaagcgcaga tcgtcgccgc gaccgtccag caggcgcacg
    33781 cccagtacga aatctcgcac gccccccacc gccgcccgcc gcggaccggc caggccggcc
    33841 gccacgcagc cccccacggt gccacccggc gcaaagcggg gcggctcgaa ggcgagcatc
    33901 tggcccgcct cggccagcgc cgcctccagc tcgaccagcg gcgtaccggc gcgcgccgtc
    33961 accaccagtt cgctgggctc gtaactcacg atgccggtgt agccgcgggt atccagcacc
    34021 tcgcccatcg gcgtgcgacc caggaaatcc ttggtgccgc cgccacgcag gcacacggcc
    34081 gagccgtcgg cagcggcctt gcggatgcgg gcagcccatt cctggattgg atcagtcaca
    34141 tgtgtctccg gatcccggat gctggcccac ccgacgcgcc cgctcgcgcc gctcgacgat
    34201 gcggcccgcg gtgcgcaggc ccatccaggt gccgatcagg gacagcacgg gcacggcgac
    34261 aaagagaaat tgcagccaac cttcaggcag ggcatccatg gcgatcagaa acggggcaaa
    34321 tcgggatgag catcgggcac gccgcgcccc atgcgctcac gaccgtactc ggcacagcgg
    34381 ttgagggtgg ggatcgcctt gccgggattg agcaggccga gcggatcgaa ggccgccttg
    34441 acccggtgga acaggcggat ctccggtgtg gcgaactggc tgcacatctg gttgatcttc
    34501 tcgatgccca cgccgtgctc gccggtgatg gtgccgccca gggccaccga gagttccagg
    34561 atctcggcac cgaaggcctc ggcgacgtcc tgatcgcccg gcgtgttggc atcgaagagg
    34621 atcagcggat gcagattgcc gtcacccgcg tggaagacgt tgatgcagcg catgccgtat
    34681 ttcttctcca tcgcctggat ggcccgcagc atctcgccca gacgcttgcg cgggatggtg
    34741 ccgtccatgc agtagtaatc cggtgagatc cgccccgccg ccgggaaggc cgccttgcgt
    34801 ccggcccaga agcgcaggcg ctcggcctcg tcgcgggaca cgcggacctc ggtggcgccg
    34861 ctgccttcga ggatcgcccg cacccgctcg atctcctcgg ccacctcttc cggtgtgccg
    34921 tccgcctcgc acagcaggat ggcctccgca tccagcggat agcccgcatg cacgaattct
    34981 tcgaccgctg cggtggcggg ccggtccatc atctccagtc cggccgggat gatgcccgcc
    35041 gcgatgatgc gggccaccgc gtcgccggcc ttgaccacgt cgtcgaaggc cgcgagcacg
    35101 cagcgcgcca gttgcggctt gggcgtgagc ttgacggtca cctcggtgac caccgccagc
    35161 atgccttccg agccggtcac caggggcagc aggtcatagc ccggcgcatc cagcgccaga
    35221 ctgccgaatt cgacgatctc gccgtcgatg gtcactgcgc gcaccttgag caggttgtgg
    35281 acggtcaggc cgtacttgag gcaatgcacg ccgccggaat tctccgccac gttgccgccg
    35341 atggtgcagg cgatctgcga ggacgggtcc ggcgcgtaat acagcccgta cggcgcggcc
    35401 gcctccgaga tggccaggtt gcgcacgccc ggttgcacca ccgcccgccg ggcgtgcgcg
    35461 tcgatgtgca ggatcttctt gaagcgcgcc agggacagca ccaccccgaa acgatggggc
    35521 tgcgcgccgc cggacaggcc ggtgccggcg ccacgggcca ctaccggcac ctgctgcgcc
    35581 gcgcagatgc gcaggatctc caccacctgt gcctccgtgt ccggcagggc caccaccagt
    35641 ggcagctggc gcagggccga catgccgtca cattcgtaag gacgcagatc ctccggctcg
    35701 tggagcagcc cgtgcgctgg cagcgcgcgg gacagggcgg tgacaaggcg gtgcttgtcc
    35761 acccgggaga aatcccgctc ccgttcactg aaatccggaa acgtgtgggc gtctgcagtc
    35821 atgtcggctc ctttgacggg ctggcgggat caagggacca ggacaccccc agccgcgccg
    35881 cggttccgat cagatgctgc tccgccgcac gccgggcagc cgccccgtct cccgctgcaa
    35941 tggcctcgaa aagggcccgg tgttcgtcct gcgcccgcag cgcccgcccg gcgataagcc
    36001 cgtcgcgact ccaggtcgga cgacgggatg ccgacagatc ctggctgacg aattccacca
    36061 gccgctgcaa cagcgcattg ccgcaggcgg cggcaatggc gcggtggaag gcatcgtccg
    36121 cctcggcacc gccctctccg gcgcgcaagg ctgcatccat ccgtgccagg gccccggcga
    36181 tcgccgccag atcagccgcc gtgtgccgcc gcgcggcccg ttcggcgact gccgcctcca
    36241 cgacgagacg cagttcgaag acctcggcgt cgctgcaggg ctccgccgac acgtcacgca
    36301 agcgaaagct cgccgcgccc ggtcgctgca tcacgaacgc accagcgccc tgacggctgt
    36361 ccaccagccc ctctgcccgc agtcgcgcga tggcctcccg caccaccgtc cggctcactc
    36421 caaactgggc ggcgagctgg gactcggagg gcaggcgacc gcccggcgga aaatcaccgc
    36481 gacggatgcg gcgctcgagg tctgcaccaa cctcttcggc gagacggccc gggcggagca
    36541 caggggtcag gggcacaacg gacattcgtc tacttgtctg acaaattatc ctgaagtgta
    36601 agcgcctggc gccgatttgc aatcccacgc ccgccatcgg agcctcgccg gtgcatgcag
    36661 acgccgcccg ccgtcgcgac acgcttcgcc agcgtgggcg ccacgcccga tccgccgccc
    36721 tccgcccgcg cgcaatccgg cgcctcagcc tctgtcgacc cagtcttccc gccaggcgaa
    36781 ggaatcctca ctgcgcccgg tcggcacgta ctcgcagccg atccagccgg cgtacccaag
    36841 cgcctccagg tgaccgaaca ggaaagggaa gcggatctcg ccggtgccgg gctggtgacg
    36901 cccgggattg tcagccagct ggatatgacc gatctgcgcc agatgctcac cgagggtgcg
    36961 ggccagatcg ccttccatca cctgggcgtg gtagacgtcg tactgcagac gcaggttcgg
    37021 gcgcccggcc tcggccagca gcgccagccc atcgtggctg cggctgacga aaaagcccgg
    37081 cgtgtcacgc gtattgaggg gctccagcag caccgtgcgg ccgctttccg agaggcggtc
    37141 cgccgcgtag cggaggttgt cgatcaaggt ggcgcgcaac accgcgtccg ccacgccgct
    37201 cggccgcagg cccgcgagca cgtttacccg cgggcacgcc agggccgcgg catacgtcgc
    37261 ggccaggccg accccatcgc gaaactccgt tctccgttcc ggaagacagc ccaagccccg
    37321 ttcaccgccc gcccagtctc ccgcgggcac gttgaagagc accacctcca cccccgcgcg
    37381 gcgcgccgcg tcgaccacct catccaccgg aaacgcatat gggaaatgaa attcgacccc
    37441 gcggaatccg caggccgcgg cggcctcgaa gcgttccaga aaagggcgtt ccaggaacag
    37501 aaaggacaga ttggctgaaa aattaagcat cgcgagcccc gggacacgga aaatcctcgc
    37561 caagcttacc ttttgacgct tcgttcgggc acccgtggca caaaccctac ggtccgatgg
    37621 ggtatcatcg taagcttgac ccaaaaccgt aacgaagttc caatgatccg caaactgctg
    37681 cgccgcgtgt ttcgccgcgc cgcacccgcc aacgccctgc ccgaaccggc cgtgatcccc
    37741 gctgcccggc acggcatccg ccgcgaccag ctctctcccg ccgccatcaa ggtctgtacc
    37801 gtgctccagg acgccggtca caaggcctac gtggtgggcg gtgccgtgcg cgacctcatc
    37861 gtcggccaga cgcccaagga ttttgacgtg gccaccagcg ccacgcccga agaggtgcgc
    37921 gccctcttcc gccgctcccg catcatcggc cgccgcttca agatcgtgca cgtcatgagc
    37981 ggcccggaga ccatcgaggt ctccaccttc cgcgccatgc aggacgcgga atccaccgag
    38041 accgacgaac acggccgcgt gctgcgcgac aacgtcttcg gctccatggc cgaggacgcc
    38101 acgcgccgcg acttcaccgt caacgccctg tactacgacc cctccgacga aaccgtggtg
    38161 gattaccacc acggcgtcgc cgacctccag cagcgcaccc tgcgcatgat cggcgatccg
    38221 cgcgtgcgtt accgcgaaga cccggtgcgc atgctccgcg ccgcgcgcct gggcgccaag
    38281 ctgggtctgg gcatcgaccc ggcggcccgc ggccccatcc gcgagatggc cgatctcctc
    38341 gaaaacgtcc cgccggcacg cctcttcgac gagatgctga agctcctgct gtcgggccac
    38401 gccgtcaagt gcctcaagca gctgcgcgag gaaggcctgc accacggcct gctgcccctc
    38461 ctcgacgtca tcctcgagca acccctgggc gaacgcttcg tctggttgtc cctcgagaac
    38521 accgacgagc gcgtgcgtca gggcaagccc gtgtcccccg gcttcctctt cgccaccctg
    38581 ctgtggcacg aggtgctggc cgactgggag gcccgcaagg cggacggtca gcacgcccag
    38641 ccggccctct tcgaggccat ggacgcggtg ctcgacaaac agtccggcaa gctcgccatc
    38701 acccgccgca tcgtcggcga catcaaggac atctgggcgc tgcagccgcg cttcgacaag
    38761 cgcgccggca aggcgcctta ccgcctgctg gagcaggccc gctaccgggc cggctgggac
    38821 ttcctgcgcc tgcgcgccca ggccggcgag atccccatgg aactgcccga ctggtgggac
    38881 gccttcgccg acgcccaccc ggacgcgcgg gaagccatgc tgctgcccga aaaggccggc
    38941 agcgcccccc gcaagcgccg ccgccgcaag aagcccgccg ccgccatcag cgaatgagtt
    39001 ctgccgtgtc cgccggcacc cgcgcctacg tggcgctggg ggccaatctc ggcgaccccg
    39061 tcccgaccct gcgtgcggcg gttgccgacc tgggcgccct gcccggcacc tcggtcgtga
    39121 tccgttcgtc cctctaccgc agtgcgccgg tgggtctcgt caaccagccg gatttcttca
    39181 acgccgtggt ggccgtggac acgtccctgc cggccctcga cctgctgcgc ggcctcctcg
    39241 ccgtggaggc ccgccacggc cgcgtgcgca gtgtgcccaa tgccccgcgc accctggacc
    39301 tggacctgct gctccacggc gaccaggcat gcagctcggc cgaactgatc ctgccccacc
    39361 cgcgcatgca cgagcgcgcc ttcgtgctcc tgcccctgct ggagatcgca cccgacgtgt
    39421 ggattcccgg ccgcggcccc gcccgcgagg ccctccccgc ggtggccggc caggccatcg
    39481 cccgcgccga agccctgtga tgccgatctg gctgcagacc gccctgctgc tggcggcctc
    39541 caacgtgttc atgaccttcg cgtggtacgg ccacctgaag aacctcgcct ccgccccgtg
    39601 gtacatcgca gccctggtga gctggggcat cgccctcttc gagtaccttt tacaggtgcc
    39661 ggccaaccgc attggcgccc ggcagctcga tctgccccag ctcaagatcc tccaggaggc
    39721 gatcaccctc acggtcttcg tacccttcgc cgtgttctac atgcagcagc ccctcaagct
    39781 tgactatctg tgggcggccc tgtgtatcct aggcgccgtc tacttcatct tccggacctg
    39841 acggcccgcc cgcaccatgc tggaaaaagc ccgctacatc gtcgtggaag gccccatcgg
    39901 cgccggcaag accaccctcg ccaagcgcct cgcggagcgt cttcacgccg atctgctgct
    39961 ggagaatccg gccaccaatc ccttcctcgg gcgcttttac caggacgccg agcgctgggg
    40021 cctcgccacc cagatcgcct tcctgttcca gcgcagcgaa cagctcgccc tgttcacccg
    40081 cgaccacgac cccttccgcc gcgcggtctc cgacttcctg ctggacaagg atcccctctt
    40141 cgccgaactc aacctggacg tggacgagct ccagctctac cgccagctct tcgagcgcct
    40201 caagcccacc gtgcgccagc cggatctggt gatctacctg caggcccagc ccgacatcct
    40261 catcgaacgc gtgcgcaagc gcggtgtcga ggccgagcgc aagatcagcg aatcctacct
    40321 gcagcgcgtc gccgagcatt acgcgcgctt cttccaccat tacgatgcgg cgcccctgtt
    40381 catcgtcaat gccgaatccc tcaacccggt ggacaaggac gaggatttcg aactgctgta
    40441 ttcccgcctg ctggccatgc gcagctatcg ggagttcttc ggctacgcgg gctgaccagc
    40501 ccgccgccag acgtccgccg ccgcctgccc cgcttccgcc ctcacccgcc ccttcgccgg
    40561 aaccccttgt gcgccgcaca ggatttgggg ttaactgccg cccgcaagga gaagccgcca
    40621 tgacctacct ccaggacacc gccaaacccg tcaccctctt tgaactgggc aagatgcgcg
    40681 ccgaagggcg ccggatcgtc atgctcacgg gctacgacgc cagcttcgca tccctgctgg
    40741 agcgctgcgg cgtggatgtg atcctcgtcg gtgactccct gggcaatgtg ctgcagggcc
    40801 agaagaccac cctaccggtg accatggagc acatggtcta tcacaccgaa tgcgtcgccc
    40861 gcggctgcag ccggcccttc atcgtcgccg acatgccctt cggcagctat gccgagggcc
    40921 cggaacaggc tatccgcaac gccgcccgcc tgatggccgc cggcgcccag atggtcaagc
    40981 tcgaaggtgg cgccgtcatg gccgagaccg tgcgtttcct tgtcgaacgg ggcatcccgg
    41041 tgtgtgccca catcggcctc acgccccagt ccgtgcacca gctcggcgga taccgggtgc
    41101 agggccgcag tgactcgggc gccgccacgc tcaaggccga cgccttcgcc ctggagcagg
    41161 cgggcgccgc cctgatggtg ctggagatga tccccgccgc cctggccacc gatctcaccc
    41221 gcagcctcac caccatggcc accatcggca tcggcgccgg cgtggactgc cacggtcagg
    41281 tgctggtgct gcacgacctc atcggcgtct tccccggccg ccggccgcgc ttcgccaaga
    41341 acttcatgga aggcgccgcc agcctggacg acgccgtcac ccgttacgtc accgatgtgc
    41401 gtgagggccg tttccccggc cccgaacaca gctactgatc ccgccatgca gatccatcct
    41461 accatcgccg gcctgcgcgc cgccctggcc ggcgccggcc gcgtcgcctt cgtgcccacc
    41521 atgggcaacc tccacgacgg gcacatcgcc ctgatgcgcg aagcccgccg acacgccgat
    41581 gtggtcgtcg ccagcatctt cgtgaatcgc ctgcagttcg gaccccggga agacttcgac
    41641 aagtacccgc gcaccttcgc cgacgactgc gcgcgcctca ccgacgccgg cgtggatcac
    41701 ctcttcgcgc cggacgagac cgagatgtac cccgcgcccc agcgctacca cgtagagccc
    41761 gagccggccc aggtgagcat cctcgaaggc gccgtgcgtc ccggccactt ccgcggcgtc
    41821 gccaccgtgg tgctcaagct cttcaacatc gtacagcccc aggtcgccct gttcggcaag
    41881 aaggactacc agcagctcat ggtcctcacc aacatgaccc gcgagctggc actgccgatc
    41941 cggattgtcc ccggcgagac catccgcgcc gccgacggcc tcgccctgtc gtcccgcaac
    42001 ggctacctga gcgccgccga gcgggccgag gcgccgcgcc tctttgccca gctcagccgc
    42061 atccgcgagc gcctgcaggc cggcgaacgg gatctggccc ggatggaggc cgatgcggtg
    42121 gccgagctcg ccgcccacgg ctggcagccc gactacatcg ccatccgtcg tcgcagcgac
    42181 ctgcaggcac ccgaaggcga cgagcccctg gtcgtcctcg ccgccgcccg cctgggcagc
    42241 acccgcctca tcgacaatct ggaactgtaa acgccacaac gcccgccccc gcgctgtcgc
    42301 ggatcggcgg gcgtcgcggc cgggtaacgg gtttcaggcg gtgggatgga attccgccat
    42361 caccgtcggg ctgccaccct cttcggcacg ccggacctgg ccgagcatct cggcgatttc
    42421 catgctgatg cggatgtctt cgatcaggaa ctggcgcagg cccacgcaca gggccatctc
    42481 gctgatcgac gtctgcgccg cgcaggcctt gcggatggcg cgcaccactt cggtataaac
    42541 cggatcgcgg gccatcagcg aggcgatgcg cacccgtacg tgggcggcca tgcgcgggtc
    42601 gaagcggcgg ctctcgaggc ggcgcaggac tgaaaccgtc ttttcgcggg ccgagcggta
    42661 atgacccagt tcctgttcgg tcgcgaggcg acgcagcgtg tcatgccagt ccggcgtgac
    42721 ggaagctgcc actcgcgcgc ggtcgcgcca ggcctgggcg ctgatcgccg ccagggcgac
    42781 gatgccaatc agacagaaaa gtgcgacata catgatcggg ctccatgcaa cggggttgtc
    42841 atgcgtccag agtaggacgt gcccccgtct ggcatggcgg acaaaatcac gcttttccgt
    42901 ggatgactgt caaatcccca caactttggc ccacgtcgtt aagtaggtca cgccacgccc
    42961 tgcaggtaga cgccccccgc ggcgctcatg catcgcgcag ggccgacacg atgttcatgc
    43021 gcgccgcccg cgccgccggc atgaagccac cgatgagtcc catcgccagc gcaaagatca
    43081 gcgtcttgac gacgatggcc ggcgtcaggc ggaagctgaa ggccagctct gaaaacgatt
    43141 gccagttcat cgtcgagatg gtgaagaact gcatcagcga cgccgccgcc agccccgcag
    43201 ccccacccag cagggccagc aacaggctct ccgtcagaaa ggccagcagt acactgccgc
    43261 gccggaagcc cagtgcccgc agggtgccga tctcggcagt acgggtcgcg accgccgcgt
    43321 acatggtgat cgccgcaccg atcattgcgc cgaccgagaa gatcaccgac agggtcatgc
    43381 cgaggatgct gatgaagttg gacagcatgg ccgactgatc ggcgtagaac gcccgctcgg
    43441 tcttggcctc caggctcagg cgcggatcgt cgtcgatcag cgcgcgtacg gcggggaagc
    43501 tcgccgggtc ggccagacgc agcaccacgg acgaataggc cgggcgccgg aaggcctgca
    43561 tcagctggtc cgcgtcgccc cagatctccg attcgaagcc gctcttgtcc gcctcgaaca
    43621 cccccaccac gcgccagtcc cgcgcaccga aacgcagcgt gctgcccagc ccggtgccgt
    43681 cgaagcggcg ggcgatgctg gccccgacga caatctcggc cgagccgggc cggaacatcc
    43741 gcccttccac cagcgccagg gtcggccgca gggccgcgcc ctgccccccc acgccgcgga
    43801 tgatcacgtt ggccggcttg ccgccatgct tggggagcgt gatcaggaca acggtctcct
    43861 tggagacctg aggcagcccc cccgccgcca gcgccacctg aggcaaagtc tccagcagcg
    43921 ccgcctgatt gcgctcgatg atgctctgca cctcggtttc agaccccttg cggatcacca
    43981 ccacgttgtc cgcctggccc gtgtccacca gcgcctcctt gaggccctgg tcgagcatca
    44041 gcaccgcagc gaagacgaac accaccaggg ccatgccccc ggccgtcagg gccgtggtca
    44101 ggcgccgcgc ccacaggttg cgcaggctgt aggccagggg aatccgcttc atgcctcagc
    44161 ccaccgcccg caggccgtcc acgatgcgca ggcggcccac ccgcagcatg ggcagcagcg
    44221 ccgccaccat gcccaccgtc agcgcgcagg caatctgtag cgccacggtt tcggtagaga
    44281 cgttgaacac cgggaagagc gtgcccatct tcgcagcaaa ggcgtccgcc acgggaaaag
    44341 tcaaagcgat gccgatggca ccgccggtga gcgccagcat caggctctcg ccgaggatca
    44401 gcagtccgac gaaggccggc gcgaagccca gtgccttcag ggtggcatat tccgccgtac
    44461 gttcgcgggc ggtcatcgcc atggtgttgg ccatcactgc catgatgatg aagatgacca
    44521 ggtaggacac gatgcggatc gccaccacga tggcctccgt catcgccacg aagctcagct
    44581 ggaaggcctt ttccgtctcg gtgagggtct cggcgcggga attggcgaac tcccgatcga
    44641 cagcccggga cacggctgcc acctggtccg gcgacgccac ttcggtgacg aacacaccca
    44701 cgttgttggc acgtcccgga tagcgctgct tgatggtctc gtccagatac tgccaatgga
    44761 acagcatctg ggactgatcg gtgcccttcc tggcgccgtc gtagatcgcc cgcacgacga
    44821 aatcccagtt gccgggatag atcgtgccct tgagcgtgag gttgtcgccg accttgaagc
    44881 cgtaggtctc ggccagcttg cgccccacca cgcagccacg acgatcccgc agaaaatcgc
    44941 ggaaggcccg ttcgtccacg acgaattcag gatagatgcg gaagtagctt tcaccgtcca
    45001 ccgcaaactg ggcgaagaag ttcttcggct ccttgtagat gccgccgaac cagctggccc
    45061 aggacagatc gcgcacgcct tccactccgc gaatgcggtc ccgataggtg agcggcagcg
    45121 gaaacaccag ggagatggaa ctgcgcgtga tcagccggtt ggccgaggcg ccttccgccc
    45181 ccgcgtacca ggcatccacg acggtcccga gcaggccata ggcgagaatt gccaccacca
    45241 tgcccagcac cgtcaggccg gcgcgcaaac ggtggcgcca ggcattgcgc aggatcatcc
    45301 gcaggaaagc cgtcagcatg ccggcgccat cccggtttgc acaaaaaacc tgtatgccct
    45361 cttgcgctcc gccgcgcttc gccccaagct atgcaggctc acagggtggc tccggacgcg
    45421 atgctgcccc tcagaccaag ccaacaatcg ggaggaggat ccaaaagaat ggcaaccacg
    45481 gtccagcaga ttctgaacaa caaaggcggc acctgtcacg gtgtcgcgcc cgacaccacg
    45541 gtgctcgaag ccctcaagct gatggccgag cgcaacatca gtgctgtgct ggtcaccgaa
    45601 ggcccgcggc tggtgggcat cttcaccgag cgcgactacg cccgcaaggt ggtgctgcgc
    45661 ggcctggcgt ccaaagacac caaggtcggc gatctgatga cccagaacgt gctgacggtc
    45721 tcgccgagcc agaccgtcga ggaagtcatg ggcatcatga ccaacaacca catccgccac
    45781 ctgccggtgg tggaccacgg cgtcctggcc ggcatcatca gcatcggtga tgcggtcaag
    45841 tcggtcatgg agcagcagca ggcgaccatc gagcagctcg caggctacat cgccggtggc
    45901 atgagcacct gatccacgcg tgcccgcccc ctcggccggc acgggtcgat ctgcaagagc
    45961 cgcagcctgc gctgcggctc tttcacgtcg ccgtggctgc gtcatgtccg caccgcctcg
    46021 cgccccgaca gctcgccctt ctccaggtgc atgatcgcgt gggccgccgc cgccgcgcgc
    46081 tggtcgtggg tcaccatcac gatggtcttc cccgcctccc ggttgaggcg ctgcagcagc
    46141 ccgaggatct cggtggcgga ctcccggtcc agatcgccgg tgggctcgtc agccacgatc
    46201 agagtcgggt ccgtcaccag cgcgcgggcg atagccaccc gctgctgctg cccgcccgaa
    46261 agctcgcccg gcgtgtggtc catacggtcg gccagcccca ccatgctcag cgcgaaggcc
    46321 gcccgctcgc gccgctcccg cgccgacagc gtggtgagca gcagaggcag ctccacattc
    46381 tcgagcgcgt cgagcaccgg catcaggttg tagaactgga agatgaatcc aatgtggcgc
    46441 gcccgccagt cggccagctc ccgctcggcc agacgggcga tgtcctcgcc ggcaacgatc
    46501 agttcgccgc tgtcggcccg gtcaatgcct gcgatgaggt tgagcagcgt gctcttgccc
    46561 gagccggagg gccccatcag ggccaggaat tcgccctccg caatgtcaaa accgatgcct
    46621 gccagcaccg gcaccacctg ggcgccgcgc cgataggcct tgcgcacatc gcgcagacgc
    46681 acaatcggcg cgccggcagc gctcccgcgg gcgtcggtca cttgcccccc gccttcaccg
    46741 cggccccgtc gcgcagggat tccggcggac gcagcaccac cttgtcgccg gacttgacgc
    46801 cctgcacggc gaccagttcg ccgatcgttg cgcccgtctg cacgacaacg cggtgggccc
    46861 gcccgtcctc cacgcggaac agcgccactt ccgagccttc gcgccgcagc gcatcttcgt
    46921 gcaccgccac ccgtggcgtg cgctcggcgt ccgccagcgc cttgtccagg aaggcgatct
    46981 tggcgctcat ctctggcagg acgcgcggat ccggatcgag gaagcggacc ttggtcagca
    47041 cgctcgcctt ggcccggtcc accgtcggca cccggcgcga cacctcgccg cggaagcgtt
    47101 gctccggaaa ggcgtcgagc tggatctcac agggctggcc cacgcggatg cgccccaggt
    47161 tggcctcgga gacgtccgcc tccacctcca gcgtgcccat gtccgccatg ctcaccacgg
    47221 cgcccttgct gtcggcggcg gcggaaaaat tggtgatcag gtcgcccacg ttggcgttct
    47281 tggtgaggac cacgccgtcg aagggcgcgc ggatctcggt ctgggccacc gccacacggg
    47341 ccgcccgctc cgccgccacc gcagccgtct cggcggcctg ggccgacccg agcgccgcgc
    47401 gggccttcgt cgcgcgggcc tcggcctgat ccacctgggc ctgggcgacg aagttgcggg
    47461 cgcgcagatc acgggcgcgg ccgagggcca gctcggcatc cttgagctcg gcccgcgcct
    47521 gccccacgtt ggcccgcgcc acccccacgt tggctgccgc ctgctcggcc tgcgccacca
    47581 cgtcgcgctg ttccaggcgg gcgatcagct caccggcctt gaccaccgag ccctcgcgca
    47641 ctcccagcca ttccagtcgc ccggtggcct tggaggccac cgatgccttg cgctgggcca
    47701 ccacgtagcc ggtggcgttg aggacggtca cgccctgata gggccacgcc tcgccgacgc
    47761 tgacggtctc gaccgacacc ggcgcattga agtgcccgat cgcagcggca agcgcggcca
    47821 ccaccaccag tgcgccaccg atcagccagg cccgggcgcg tcggcgagac ggccgcggcg
    47881 cgttgcggtc gatgctcaga cgggacaggt cggattcact catggcgtgg cgggccggcg
    47941 atggatgtgc cggtcattct accccgccgc catgacaggc cgcgtcgtgc ggcaggccta
    48001 caggcgctcg ctctccggca gcaccaccgg gctcgcctga tccggccggt agccgcacat
    48061 cccggcatag acctcgcgga tgcgcgccat gtccgccgcc acgttgcccg tgaggcggac
    48121 gtaagcggca atccccaggc gcttgcgtcg gtagtcgatg aagcccagcc ccaccggcac
    48181 gtccgccgcc cgcgcaatgc gatagaagcc ggatttccag ccggcggtgc gacggcgagt
    48241 gccctccggc gcgatggcga ccagcaggtg atcgcgggaa cggaacgccc ccgccatgcc
    48301 ctccacgaaa ccgcccggct gtccccggtc aacgggaatc ccccccaatc ggcggagcag
    48361 cccgctcacc ggccagcgga acagggtgtg cttgccgatc cagccgatcg gccagcccag
    48421 ggcaaaacgc accagcaggc ccagggggaa gtcccagttg gaggtgtggg gatagacgac
    48481 gatcaggcaa tgggccgcag gcaatggcgc cacatcgagg gtccacccca ggcgtcgcag
    48541 cagggccccc gcccaggggc gaggatggac agggaaggtc gcggacgggt cgattggcat
    48601 gccagtagga tttcggacgc ggagcgggga taattcggga agcgcaggag ggggcgatca
    48661 tgctcccgtc cctgcgtcga ctcaagcacc gccgcgcccg gcgcctcgac gcgcccgcca
    48721 aaacgatcca tgccagccac cgcgcccgcc ttccgtccgc cccccatcct cgacatcgaa
    48781 gcgtccggct tcggaccatc cagctatccc atcgaggtcg gcctgatcct gccggatggg
    48841 tgcacctatt gttcgctgat ccggcccctt gacgcctgga cccactggga cgcgagcgcc
    48901 gaggcgatcc accatgtcgc ccgcagcacg ctcgtggccc acggccgcag ccccgccgac
    48961 gtggcccacg ccctcaacct ccgcctgcac ggacagacga tctactgcga cgcctggtat
    49021 cacgacttca cctggctggg ccgcctcttc gatgccgccg atctgcccca gctgttccgc
    49081 cttgaagaca tccgcagcct gcttggcgag accgacatgg aggtctggca tgccaccaag
    49141 accgacatcc tggcggagct gggcctgcaa cgtcaccgtg cctccaacga cgcccgcgta
    49201 ctgcagcaga cgctgctgcg ggtgaaggga tggacggcgc cgatcgccgc cgaagcgcag
    49261 ccctgcgacc ggccggtctg aagtccccgg ccggtcccgg gctcagaacg acctgcggta
    49321 cgccacgcca cccggcgaca acagccagtg gccggtcttc acgcccacca gggcgccggc
    49381 ggcgtcccag ctcagatcct tccacgagaa gcggttgcac gcgcgctgcg agtcgtagac
    49441 ctcctttgcc acaccggggg ccagcgccag ggcaaaggcc gtccacggat cgctgctgat
    49501 ctgggacgtc gccacgccga gggctgcaga caccccgaaa tgggccgcct tgtcgcgccc
    49561 ggcccagtcg tcctcatagg gaccgcaggc gtgggcagtg gaagacagca gggcaaacag
    49621 cagactggcg gtgcggagcg tcacgggaaa cctcgcggaa caggacgggt ggaaaccgca
    49681 gcggacggcg ccggaccggc gcgcccgtga tcccggcggc gtctgcggcc cgtacatgcc
    49741 atcgcgatgc gcgacagcag cgcaccttgc cagagaaccc cacacggccg caaccgtttc
    49801 ccgccatcgc agcatgcacg cggggcggag gccctcacag cctccgcccc gcgcgttcag
    49861 ccgcaggcac gtgcgccgcg cctgcacccg acccttactt cacgactgca cccgccagcg
    49921 ccgcggcctg gtcagcgggc ttggcctcgg ccttcaccgc cgccttggcc ccgtccatcc
    49981 gcgcgccctg cggggcaacg gcgtccttca cccggaccgc ggcggacgcc tgcgcttcgc
    50041 gcggtaccgc cgcgcgcttc acagcctccg ccttcccggc agcatgggtg cggacgtgct
    50101 gctccatcgc tgtctgtccg gctgcggcgg caccggcccc ggccttcacg gcatcggccg
    50161 gcgttgcccg cgggtcggcg ggcttgacgt cggcagcgtg ggcggcaccg agggccagcg
    50221 caaggctcag ggcgaagagg gacttgttca tggacgttct cctgtcggtt cgggggttta
    50281 gggcggtcac cgcatcgcgg cggctcgccc ttccctcaac gcggcgcctg tcccccggga
    50341 gaacaggcaa acggtaacgt cctgtaaggc tgtaacagac gctcagagcg gccgcttgac
    50401 cgtcagggca ccccggatc
//
LOCUS       DAOQMM010000013        49307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_153_length_49307_cov_46.307703, whole genome shotgun sequence.
ACCESSION   DAOQMM010000013 DAOQMM010000000
VERSION     DAOQMM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 49307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49307
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153_length_49307_cov_46.307703"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1511..3898
                     /locus_tag="PLH85_03840"
     CDS             1511..3898
                     /locus_tag="PLH85_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HNB63720.1"
                     /translation="MNILSVVDEDGALFSRVEYDRNNRVVYSGRGGDQSSYTFKYSED
                     KWFPTSTVVTDPSGVQRTYSSVLDKSQIFPTGVSKPGGAGCNASSSNIDYDTFGNAIS
                     RTDFNGMKSCHGYDASRNLETSRIEGFASSDACPADVLSSAPSSTQRKTTTQWHPFWR
                     LETRQAEPKRVTTWVYNGQPDPTNGNALASCAPATAKLPDGQVIAVLCKRIEQATTDA
                     TGAQGFGASADGPARIWAYTYNAFGQVLTEDGPRTDLADVTKYVYYTDTTANWTQGDL
                     RSITNALGHVWTFTQYDKAGRLLAATDPNGTALTYTYTPRGWLASRSVAGQTSTFAYT
                     GWGGLALVTLPDGTSTSYTWDAAHRLTSVSDSAGNSTRFVLDPSGNITSSTTTDAAGN
                     VARQTRTEFDALGRPWKHYDAAGHAFEVWHDAMDRVAKTIDPKGRTTTFARDTLGRLQ
                     DITDAQTTPGHTGFAFDARDQMTRQTAPNGAVTQLSVDGLGNTLQEVSANRGTLRFTY
                     DAAGNLLTRTDARGVTANYTYDALNRIRKRITKNSAGVVTETLDYIWDGASGCTYGRG
                     HLCQITDGGGVTNFAYDARGNRISERRSEAGRSYTTQYTLNGADRIVALVTPTGSLLD
                     QARDAGGRVAALTATAGGSSIPVASAVRYDGAGNVVSLSQGTSLSQLMQRNADGQLEA
                     NGLGIPVTGLGLSLGKRFIRPGDGLDFTLTLTPAQASGTVFLCQLDCQGSNQLAVIPV
                     TGGTTTGTLTGLTKGVHHLSAVLVPNPPYAASLSESRRVLVGVPPTLFFDDLFLQ"
     gene            3917..4267
                     /locus_tag="PLH85_03845"
     CDS             3917..4267
                     /locus_tag="PLH85_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63721.1"
                     /translation="MNRRALILTLMMTLPALPAAAGTLACNGTIAQVMTTASGDLRVV
                     PSWRNDWVTLCNVVSPWKGVPVDACKRWHAQALAAQIAQTNTQTAYASTAATACAQMA
                     VASAADAPDTFSNN"
     gene            4280..4633
                     /locus_tag="PLH85_03850"
     CDS             4280..4633
                     /locus_tag="PLH85_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63722.1"
                     /translation="MFRSILRLALLCGTLSSPVAQSAALSCTGTLTDVANSAAGELRI
                     KPSWRGDWITLCSTAVVLNGVQTEACKRWQAQALAAQLTAANTWVQYDNPVAPSCATM
                     GTLGNADRPGYLVTY"
     gene            4648..6030
                     /locus_tag="PLH85_03855"
     CDS             4648..6030
                     /locus_tag="PLH85_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNB63723.1"
                     /translation="MTGLSTLRACLLGTSCLLAASAAHADTYNYDELGNVRQIQNDKG
                     VRTYTFDEVNRLKTESGLAGVRTHTYDPNSNRLNDGAPSPTTASFAPASDRIATLNGV
                     TVTLDAAGNLLGDGTYTYAWNDAGQLLSVSKAGQLVAKYYYDYKGRRTRKATTAIAPQ
                     GAQVFVYHYDASDHLIGETLGDATPLRTYVWLNDQPNAVIEHPALSTPQRTLYLQVDH
                     LGTPRLAKDSTGKIQWRWDSDGYGSTAADEDPDGDGKKTTINLRFAGQYYDVESGLHY
                     NWNRYYSPRLGRYISSDPIGVEGGGNTYGYVGGNPLSYFDVLGLSPMSPTEIAQIIKD
                     NNHSPLSNEMVMCLMWQESGFDPDVENDGARGLMQIRKNQALAQVNKKSGPFAWQDLF
                     DPSTNTEVGTLYLNWVSQRPGDLDRNIQRRYGTGDDYPVAKMRQCEKCLQETCSKDGD
                     VKKCLAKVHR"
     gene            6006..6581
                     /locus_tag="PLH85_03860"
     CDS             6006..6581
                     /locus_tag="PLH85_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63724.1"
                     /translation="MLSKSASLIFRLLFVVAALGAQCSFANDACSSFSAADITTYLPD
                     GEGYWMRGRNGPNGSAISVCTERGKGVPVAFISEDSAQEFADLHILSIQSFYGLLIVT
                     QESGAAYRIRFFAYDIKANQVREMASDGSKFLPSLGYLGSELQPVVGVIHSALDSRVS
                     SQPLPKEEVLYFLDKSKMNISSKRKAWSAKR"
     gene            7018..7401
                     /locus_tag="PLH85_03865"
     CDS             7018..7401
                     /locus_tag="PLH85_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63725.1"
                     /translation="MLLRHEVIMNSFNDFLLRMGGMHDSIVERLMWSPDEKRIELQFD
                     DLYRNFNGLPEYPGRQPGVIVLHGVSNLSLTLDSDGPLRVFEFLPEEGESDVVVLTFS
                     PTGKLRVQYRSVDHPACLINAVGRS"
     gene            complement(7766..9727)
                     /gene="parE"
                     /locus_tag="PLH85_03870"
     CDS             complement(7766..9727)
                     /gene="parE"
                     /locus_tag="PLH85_03870"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNB63726.1"
                     /translation="MTQKHYTADSITVLKGLEPVKQRPGMYTRTEHPLHVIQEVIDNA
                     ADEAMAGFCRRIGVTLHADGSVSVADDGRGIPVEIHPVERVSTVEVVFTRLHAGGKFN
                     KTDEGSAYRFSGGLHGVGVSVTNALARRLEVEVVRDGGRHRLVFAGGDVIEPLVRIGD
                     APKKASGTTVRVWPDAKYFDAAELPRGELERLLRSKAVLMPGLDVSLAVEGGDTRNWR
                     FDDGMRGYLNESLTGEPLIPLFSGEKYAPKGDETFAAGEGAAWAVAWTAEGAPVRESY
                     VNLIPTPAGGTHEAGLRDGIFTAVKNFIDHHALLPKGVKLTADDAFGRASFVLSARVL
                     DPSFQGQTKERLNSRDAVQLVSRMVRDPFELWLNEHVEYGKKLAELAIKAAQARARAA
                     QKVEKRKSSGVAVLPGKLSDCESEDIARNELFLVEGDSAGGSAKMARDKDTQAILPLR
                     GKVQNAWEVDRDRLYANAEIHDIAVALGVEPHALDDTADLSELRYGKVIIMSDADVDG
                     SHIQTLLLTLFFRHFPQLIEHGHIYVAQPPLYRIDVPAQGKKRPPRRLYALDDRELAT
                     LRDRLVHEGVKAEHIEVGRFKGLGEMNPEQLRETTMDPATRRVLPVQVRPAALEETRR
                     TFTMLMGKGEAAGRRAWMEDKGDSVEADV"
     gene            complement(9831..10316)
                     /locus_tag="PLH85_03875"
     CDS             complement(9831..10316)
                     /locus_tag="PLH85_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNB63727.1"
                     /translation="MRPLPRLACLALLLAALLAASAYADELRHVSLPDARYADAREAL
                     VDAIEGEGLIPSPPSRFGDMLARTGPALGQDGAVYGDAEVLHFCSARVAWQLARENPV
                     NIAQCPLSMAIYTRAGEAGTVYLAWRPTQGDSAGSRAANALLERIAAQVSDNTGRARP
                     R"
     gene            complement(10378..11292)
                     /locus_tag="PLH85_03880"
     CDS             complement(10378..11292)
                     /locus_tag="PLH85_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB63728.1"
                     /translation="MNAPPLASLNDLHTFATVARTRNFRRAAAELGVSPSALSHALRA
                     LEARLGVRLLNRTTRSVAPTEAGQRLLDRVAPALRELHGALDAVNAFRDSPLGTLRIN
                     APRAACAWVLAPLVSRFLAEYPGMRVELVADDRFVDIVAGGFDAGVRFGESVQQDMVA
                     VPLGAAQHFVVVAAPAYLAARGTPDCPRALTAHSCIRVRFPGGQYYRWEFRRGSEQVE
                     IEVDGPLATDDMHVMIAAAEAGLGLAYVYAQYAAPAIAAGRLVSVLDDWRPPEPGFFL
                     YYPSHRLPPAGLRAFIDLARRIHPDNRP"
     gene            11398..12171
                     /locus_tag="PLH85_03885"
     CDS             11398..12171
                     /locus_tag="PLH85_03885"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB63729.1"
                     /translation="MQTSTQFPDLAGKVILVTGASSGIGAATALALGRAGARVALAAR
                     RLDSLEALAAQIRDAGGEALTVQTDVTVESQVRDAVAATVARFGRLDGAFNNAGALGA
                     AAPLHESTTDNFDAVFKANVYSVYWSMKYEIAALLQTGGGAIVNTSSIVAQIGFPGVA
                     AYTASKHAVMGLTRNAALEYFTQGLRINAVLPGPIETPMAESGFGSLEALRESMKASP
                     AGRAGVPDEVAAPVLFLLSDGARYMSGQALTVDGGYTVA"
     gene            12264..13283
                     /locus_tag="PLH85_03890"
     CDS             12264..13283
                     /locus_tag="PLH85_03890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB63730.1"
                     /translation="MNAVSVSSPLRSLGPLRVPVAPIGLGCMGMSEFYGPSDDAESLA
                     TLARALELGVTLFDTADTYGHGANERLLGRFIAEGGAARRARMVIATKFGIVREPGRY
                     ERRIDNSPAYLRAACEASLQRLGVEAIDLYFCHRRDPAVPVEDMMGALAALVQAGKVR
                     QVGLSEVSVDTLRRAHAVHPVAAVQSEYSLWSREPEQGMLAACAELGTRFVAYAPLGR
                     SFLTGTTDVAALADNDFRKHNPRFAAEAVERNRQLVEALARFAEARGASSAQIALAWL
                     LAKQPHVIPIPGTRRIRWLEQNMAAARIALDADEVAQLDALFAPEQVVGARYPEAGWV
                     GVERG"
     gene            complement(13288..13632)
                     /locus_tag="PLH85_03895"
     CDS             complement(13288..13632)
                     /locus_tag="PLH85_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNB63731.1"
                     /translation="MIAPLRVAVATKNRVQIDEHFGHADEFKIYAVGPDGIAFEEVRV
                     VEHYCQGGFGDEDKREVILRALADCTALFCAKVGDGPAKRLREAGIEPVGDYPYGAIE
                     DSLRAWFAGRPA"
     gene            complement(13834..15438)
                     /gene="aceB"
                     /locus_tag="PLH85_03900"
     CDS             complement(13834..15438)
                     /gene="aceB"
                     /locus_tag="PLH85_03900"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HNB63732.1"
                     /translation="MSIQLSLPAGMQINAPIRPGYETILTPEALELVAKLHRAFEPRR
                     QELLKARVERQARIDAGEMPDFLPETRHVREGDWKVAPVPAALQCRRVEITGPVEAKM
                     IINAFNSGADSYMTDFEDSNSPNWDNQLQGQVNLYKAIRRELSFKNEAGKEYKLNDKI
                     ATLQIRPRGWHLDEKHVLVDGQRVGGGIFDFALVFFHNAKEQIARGAGPFYYLPKMES
                     HLEARLWNDIFVMAQDHVGIPQGTIKATVLVETILATFEMEEILYELRNHSAGLNAGR
                     WDYIFSCIKKFKKNKDFCLAQRGAITMEVPFMRGYALALVQACHKRGAPAMGGMSALI
                     PIKGDPEANEKALAGIRHDKTRDANDGFDGGWVAHPGLVSIAMEEFVKVLGDKPNQFE
                     KQVEGKFGPEQWLDFRPTTPITEAGLRNNINVGIHYLGSWLAGNGCVPIHNLMEDAAT
                     AEISRSQVWQWVVSPKGVLDDGRKVTEELVRPMIAEELAKVKATVTAQGEDTATYDQA
                     AVIFDKMSLTPDYPEFLTLPLYEAME"
     gene            complement(15495..16187)
                     /locus_tag="PLH85_03905"
     CDS             complement(15495..16187)
                     /locus_tag="PLH85_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNB63733.1"
                     /translation="MAHAPRIQAVAFDAFGTLFDVYSVGLLAEQLYPGKGAQLADLWR
                     QKQIEYSFIRTLSGRYKPFWSVTEDGLVYAAARLGLELDAPRRTQLMNQYACLSPFPE
                     NLGALKALKEAGVPTAVLSNGTPEMLDVAIKSAGMNGLFDHVLSVDAVQQYKTSADAY
                     ALGPAAFGCPAERILFVSSNGWDAAGATWYGYTTFWINRSGLPQEQLDVSPVARGRQL
                     SDVVDYVARHRA"
     gene            16368..17273
                     /locus_tag="PLH85_03910"
     CDS             16368..17273
                     /locus_tag="PLH85_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB63734.1"
                     /translation="MSNFKEIEAFVSVAARGSLSAAARADGVTPAMMGRRIDALEERL
                     GVKLLVRTTRKLSLTFEGSAFLEDCQRILNDLANAEASVSLGGVKASGHIKVSAPAGF
                     GRRHVAPLVRDFLAAHGDVSCSLDLSDRLVDLVNEGVDCAIRIGDLSDSSLVSVRLAD
                     NRRVVVASPAYLALHGVPATPEDLLSHDCLSLNPQRGWVFCDPDAPGTTRVVKVSGRF
                     ECNDGTVLHEWALAGIGLAWRSMWEVQRDLAQGALVSVLDAFAAPSTGIYAVFPQNRR
                     LPLRVRLLIDHLRGSFGQPDYWRAG"
     gene            complement(17255..17737)
                     /locus_tag="PLH85_03915"
     CDS             complement(17255..17737)
                     /locus_tag="PLH85_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63735.1"
                     /translation="MVQVTFTPAIAERRDDALSLAPAPADPPEPPPVEQPPTPGADDT
                     PPSADALPLSALSSPPDLLTPVPASIWPPFPELGHRIFQLEVIVAADGKVAAVNTQCD
                     EALCAAADAYAAIVRTWEFTPGEYRKHPVPVRLRIEFELNPPPPETPTESSPTQPARQ
                     "
     gene            18026..18469
                     /locus_tag="PLH85_03920"
     CDS             18026..18469
                     /locus_tag="PLH85_03920"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNB63736.1"
                     /translation="MKRQSGVTLIELLIVVVIVAILGGIAVPAYTDHILRGKIAQVTE
                     NLAEAKVRMEQVFNSNRTYATSPGGTSCPDFSTLFDKTGFTLKMSDCPGDAGTTFTLT
                     ATGSSSSGTGGFMYSINQNGEKKSTTPATSEVQSCWLMSKTASSC"
     gene            18463..19080
                     /locus_tag="PLH85_03925"
     CDS             18463..19080
                     /locus_tag="PLH85_03925"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNB63737.1"
                     /translation="MLIPARRLRGATLLELMVTVSIVAILMAVGMPSMKDWILRNSVS
                     SAAEVLQNGLRRAESEAIRRNAPIEFLLTSGIPSSSASLTAEANAVNWAIRVVDSSNS
                     YKPVAGGYVAGFYLKDISSAVTVSGPASVIFNGMARVLDSAGVPVNGYQVYRFSRSGS
                     DKAYCVFVSQGGGVKMCDPDQASGTPRACQPLLSASQCPKAGSGS"
     gene            19077..19505
                     /locus_tag="PLH85_03930"
     CDS             19077..19505
                     /locus_tag="PLH85_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63738.1"
                     /translation="MKRRSFAGYVLLEALVGLLIFSLGLLGLIGFQASAMKIASDSRF
                     RTEAAMLADELIGKMSMSKISTVQTEYSADGSKFSDWLNNRVKGGSKLPGAAASVTFA
                     TANTNATLLATVTISWDLPGSSGRVGDSLHGVYVTQAMLF"
     gene            19518..20753
                     /locus_tag="PLH85_03935"
     CDS             19518..20753
                     /locus_tag="PLH85_03935"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNB63739.1"
                     /translation="MRRPPFPVLRDRSRQRGISLVETMIGMALGLVATFVIIQSFSSS
                     ETYRRNLAGTADATQTAAIAGGHLDLVLQEAGASLVQGRNVWGCKILVTRNKTAILPH
                     GSNYPSPFASFPTTVRVLPLAVLDGGDKGSDVIMAMVGNSGTGNRDTPFSAPAGGKSM
                     VVSNNNGMALTSSGQSPNDLFLSVPQEVAGAPGDCQVVQADSSFSNGTAVADPSLGLQ
                     VMPASVTAPQSLTTIPLNASTYGALASSANSPSAFHLGRESAPIFSLFAVNSNGELVE
                     YDLLERNGVMTFGENIFLIKARYGLDNGANGGTANDNVIDDWVSPAEDGWKLAQLMDG
                     KSSTEQKVDQIKAVRLALVTRSSQSVTSDAKVSSLTLFSDLSSTRQYTRSLSDSEQAY
                     QYQIVDWVIPLRNMKATPK"
     gene            20765..21418
                     /locus_tag="PLH85_03940"
     CDS             20765..21418
                     /locus_tag="PLH85_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63740.1"
                     /translation="MTTSKIPLHPPRRNRGAVLAIVLVVLVVMMLGAVSMLRSVDTSA
                     LLSGNLAFKRESLNRSTVGLNTAFAQFKKDDFVKYSDDSTGCNAASTGPSCTYASNWL
                     KMNYSPRLLATDGNGVPLILKDISSFDKTFNSSLVVTDKADPNVKVRFVIERMCNDFG
                     PATETKCILSENAERGGSQPLDKLGSAALPMYRITVRSDGVRNSQTYAQAVVTTRVK"
     gene            21445..25308
                     /locus_tag="PLH85_03945"
     CDS             21445..25308
                     /locus_tag="PLH85_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNB63741.1"
                     /translation="MKLSKSLSRFLLIVASASALFSAGLQDGRAASTPLADQPLLTAQ
                     VPSNVILALSVEFPTALSVAYNTKFSVDGSAYLGYFDPKKCYKYDSANNYFVPQGPSG
                     TNYTCTSAWSGHFLNWATMQAIDTFRWAMTGGNRVIDLPVNFTDTQPVKTVLQRAYAS
                     SDGSECTYFPGRGISASDRANYTDTAGVGYTDKGLWIRNGGQGYQFQVAPQIGGAANC
                     SSWGGGSGSVQSYYAQVEVCKNANGSDGKTLLESNCTMYVDPTGTKTAYRPIGLMQQY
                     KLAMNFGAFGYLYDGSSTRDGGVLRALSRPIDLEISDTGAFYPDPFGLANASRGIIYS
                     GTANYLNKFGSTSLHYKSLDPVSEMYAEVMKYLKGMDVTPSYVSTINNSMRDGFPVFG
                     ESGGPTWVDPARDTSDPSYPNSPALWCKKSYIIGIGDTNTWNDQNLSGRTDVSVPSGV
                     DTNLAGGTAKSWTDTVGTTEGYVPSGYASLGVAQPNPGHSNSFLMAGIAYYAHSSDIR
                     TDLKDTQSVDTYWMDVMEGGIYKDKNQFWLAAKYGGYKKPLNTAMGPFNSSTDFWKSQ
                     GKVIQTKSDGTVVTTGGAKSYDLPDNYFPANNPANMVSSLQSAFAQISAGTGAAAGVA
                     LSNPQVNAASGGSMTFQGTYDASTWSGNVTASNITSFKDDGTPVTTSVWDAAARLNSM
                     VASGGYSSKRKVVTLVPTKVDNATPAVADLVGKPFLYANLSTAQKLNLSKTSLGGDLG
                     DGDKVLNYLRGDRTYETTATTPNLYRLRGSVLGDIIDSKIVYVAAPNAVYSDSYNPGY
                     ETFKKNAASRQPVVYVGANDGMLHAFDATSASTGGDELFALVPYALYAGPSNTPDVDG
                     LQAIARTSYSHHYYVDSTPEVRDVDFMRAGGATQSDATKSDWRTLLVAGLGKGGKSFV
                     AMDVTSISSGLTESQVAGKVLWEFFHPDMGFSFGRPLITKTRKWGWVVILSGGYNNIG
                     GDTKAGYKPGQGVVFVVSPKDGSLLQAIYTGAGDAASPAGLAQITGYTQNYQDYTTDY
                     VYGGDLSGNLWRFDFTDSSKSVPSPTKIAQFKDPDGTPQPITVAPKAEYSADDLKRYV
                     FVGTGRLLASSDQKNAQKQTMYALRDGTRTYAFGSNTNQVALPDGVSFPVGRGSMSGV
                     SDLLAGATKSESTPMGWYYDLTGVTSGVTERVVLALQANDGVLSWVGSLPNSDPCSPS
                     GASRLYSVQYGSGQSVFYELVNGLRSPVAYLSIDQGLADITLVRYGSSIRVVGTDTKG
                     GAKVYGNTIAESSDPRVVNWRIIRE"
     gene            25370..25445
                     /locus_tag="PLH85_03950"
     tRNA            25370..25445
                     /locus_tag="PLH85_03950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25403..25405,aa:Thr,seq:cgt)
     gene            25588..28062
                     /locus_tag="PLH85_03955"
     CDS             25588..28062
                     /locus_tag="PLH85_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNB63742.1"
                     /translation="MSHSVPPQEHACYHCGLPIPSDVHLGVTVAGVERSMCCTGCQAV
                     AQSIVDGGLVDYYQRRDAMPEPVREAMPDELRDLGLFDHPDFQQGFVKPVGEHEREAS
                     LILEGITCAACIWLNEQHVARQPGVMAVEINYATRRARVRWDERRIKLSNILAAIQAI
                     GYRAYPYDAARSEQISQKERRAALWRLFVAGFGMMQVMMYAVPVYMAGEGEMTADIEA
                     MMRWASLILTLPVVLYSAAPFFRNALRDIRLRRLGMDVPVALGVGAASVASCWATFAG
                     HGEVYFDSVTMFVFFLLCGRYLEMLARQRAIRGVEELGKALPAFAERLTAFPSLEGER
                     VPVSQIVSGEVLRVRPGETIPVDGVVIEGTSEANEALLTGESRPVPKGPGVDVTGGSV
                     NVSSPLLIRVTQTGEHTRLAAIRQLMERASAEKPQVVQQADKVAAYFVVALLVLAFAT
                     AVGWWWFDPDRALWVFVSVLVVSCPCALSLATPVALTVATDALARVGVLVTRGHAIEA
                     LAKTEQMVFDKTGTLTYGDMRVEATCLLGSRSTRDVQRLAAALEQGSEHAIAHSLRKL
                     VTDAPDQLPVLSDVTVVTGQGVGGRLDSERVRIGRPEFVAEICPGTLPAELTSAQASG
                     KTVIALGGESGWLAGFFLSDVLREDAHDVFDALHGDGVTTAIFSGDTQGVVSELGKAL
                     GVTEAVGELTPEGKHVRLSALQAHGKVVAMVGDGVNDAPVLAQAQVSIAMGGGTDLAR
                     NQADIVLLSGRLGSLVDGMRLSRRALRIIHQNLWWSFAYNFTAVPLAMSGFVTPWMAG
                     LGMSASSLLVVLNALRLQRKHGKVAA"
     gene            28062..28259
                     /gene="ccoS"
                     /locus_tag="PLH85_03960"
     CDS             28062..28259
                     /gene="ccoS"
                     /locus_tag="PLH85_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNB63743.1"
                     /translation="MESLYLLIPLSIVLVFVIGVVFWWSVRSGQFDDLEGPGFRVVTD
                     DDTPRTETESPVERKGTTERS"
     gene            28950..29273
                     /locus_tag="PLH85_03965"
     CDS             28950..29273
                     /locus_tag="PLH85_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNB63744.1"
                     /translation="MGNLAYSVDKTRLEEGFREFGTVVSAQVIMDRETGRSKGFGFVE
                     MGSDGEAQAAISAMNGKSFDGRNLTVNEARPQEPRSGGFGGGMRRSGPGGGRRGPTSG
                     GGDRY"
     gene            complement(29430..31121)
                     /gene="recJ"
                     /locus_tag="PLH85_03970"
     CDS             complement(29430..31121)
                     /gene="recJ"
                     /locus_tag="PLH85_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNB63745.1"
                     /translation="MTRILSRSAPGQAVTALLDTGVHPLLARIYAARGIATRHELDYG
                     LQHLLPPNQLKGVGAAAQLLADAIEANARMVIVADYDCDGATACAVGMRALRAFGADV
                     HYIVPNRFEYGYGLTPAIVELAAALQPELIITVDNGIASVDGVAAARECGIATLITDH
                     HLPGDELPEADVIVNPNQPGCEFPSKALAGVGVMFYTMLALRAELRSRGAFDGQAEPK
                     LADLLDLVALGTVADVVRLDHNNRILVAQGLQRMRAGRMQPGLRALFAAAGRDAGRAT
                     TFDLGFALGPRLNAAGRLADMSLGIECLITDDASRALQITQELDALNRERRAIEADMQ
                     EGALAGLDGLSPGDCSSLVLFEPDWHQGVIGILAGRIKERFHRPTIAFARSHEGELKG
                     SGRSIPGLHLRDALDLVTKQAPDLILKFGGHAMAAGLTIREPDLGPFRDAFERVVRQM
                     IDPADLTRSVETDGSLEEGFYSLETARLLENQIWGQGFPAPLFDDCFRVENQRLLKDK
                     HLKLQLRKGALRIDAIRFNHADPAGAAIHAAFRLAVNEYNGVASVQLMVEEFENT"
     gene            complement(31118..32167)
                     /locus_tag="PLH85_03975"
     CDS             complement(31118..32167)
                     /locus_tag="PLH85_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63746.1"
                     /translation="MQYDLVLPGLVWPASGLAGPAADLELPALAALIGHARVHWGAAC
                     TPEAWLADRFQLAGKRIPYAWLRRLGEATTSPERSAHWLCIDPVHLHFARDKLLLADA
                     SGLDITQAEADTLIAGLNETFADIGRFEAPAPDRWYLRAEREPRPFFHPLADVTGRPI
                     QLFLPEGDDIPFWARLSNELQVWLFNHPVNAAREAAGQRAINSAWLWGAGQPLTPIAP
                     APSLQADDPYTCGLIRASGGAPRSSGPYAAPGTDGFTLLTALRRPLLHRDPDAWRATL
                     GTIERDWFAPLLAALRSRHLSRLRITAPGDRACLELDVTAASLWKFWRKPLALDTLLR
                     QTPNPASDVASTTAP"
     gene            complement(32251..33810)
                     /locus_tag="PLH85_03980"
     CDS             complement(32251..33810)
                     /locus_tag="PLH85_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB63747.1"
                     /translation="MTRFHPPRFPAFHRAFATALLLATGIVHAVGATVIHDDALRAQP
                     RSDAAIVMPLHRLSSVDIADARRDWYLVDAAGNRRGWVPAASLRLDEPPASPSAPAAP
                     TAFAASGARPRLQPHASRHALLLDLTDHDPVPPGSHATDLTARQLARALGVPDANIQQ
                     PDPVSLTPDRIRRALADLDAHAGPGDRVFVHILARTEPPRAARAPSAAALATPDGRVQ
                     VAELGRYLDTLARKAGQVLIVVDAAPPAPQTMAPDRRTDVPSRSHGLLQHWQEALPNS
                     PPNLALLASDSPDFGAASAALLACLSGDAPVTSTSGLARIDDLRRCAEAQLGPARPGA
                     RRLERVGNADLVPLPTLTPAAAPGFTPAALLRILHAQRAADRPLDVRRQDDRLLIRSP
                     RAGFLYVLHAPSDGRGFELLLPDASDSRLHLAPGVQISPSLAGTPDGPLLVLVTDAPR
                     RFVRAGFQAAGAILATPIDARSLRDLPAEVLEGDNDPRCLLAETRNLGPEQALRCSAA
                     YASALIEPRQP"
     gene            33974..35227
                     /locus_tag="PLH85_03985"
     CDS             33974..35227
                     /locus_tag="PLH85_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNB63748.1"
                     /translation="MSYEFLVGLRYTRSKRRARKGNRFISFISLVSMAGIALGVAALI
                     VVLSVMNGFQRELRTRILGVASHVQVTGFDGELANWQRIADEASKHPRVLAAAPFVQA
                     QGMLSFDQTVRGTLVRGILPDAEDKVADFAQHMRAGRLDELQPGGFGIVLGADLARAL
                     RVTVGDKVTLIAPQGLVTPAAILPRLKQFRVTGIFEVGMYEYDSSLALVHLQDAQALY
                     RMEDRVSGVRLKLDDLFKAPQVARELMGYVQADTLISDWTRAHANFFRAVQIEKNVMF
                     IILLLIVAVAAFNIVSTLVMAVQDKRADIAILRTLGASPGSIMGIFVIQGALIGVVGL
                     AIGVIGGVALALNIDVVVPAIERTFGIQFLAKDVYYISDLPSQLLWSDVGVIATVSFV
                     LTLVATLYPSWRASRLNPAEALRYE"
     gene            35220..35915
                     /gene="lolD"
                     /locus_tag="PLH85_03990"
     CDS             35220..35915
                     /gene="lolD"
                     /locus_tag="PLH85_03990"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HNB63749.1"
                     /translation="MNERTSDGVIACRGLAKTFREGDDAVSVLTDVTLSVQRGERVAV
                     VGASGSGKSTLLHLLGGLDVPSAGSVSLLGRDFSTISDAERGRLRNQSLGFVYQFHHL
                     LAEFSALENVAMPLLVRRLPREEALARAAEALKDVGLEHRMDHTPGELSGGERQRAAI
                     ARALVTRPACVLADEPTGNLDRQTAERVFGLMLALAEQHGTSFVIVTHDTALAARTHR
                     VLHLRDGRLAGET"
     gene            36055..38076
                     /locus_tag="PLH85_03995"
     CDS             36055..38076
                     /locus_tag="PLH85_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB63750.1"
                     /translation="MHLLFAPAVALLNRMRYRAKFALLGALAFAVVLFLLGQLAFSLR
                     ESMHFTEAERSALGVVPRLLTVIQLTQQHRGLSAGVLNGNEGMRIRLTAKTGELGEAM
                     KKVDAIALGADASIPMARWGEVKSAWEVLRANGLNLPPKENLTAHSRLVAMEVLALHD
                     LGDDGNLTLDPVADTYYLIDNLLKRVPEVTERLGRLRALGTGVLAAKSLDEQKRYDIS
                     VQLGELDMAVEALNENFDRAIRFNPALKGSLGALRDGFNAGTGKVLDQLQNHVLKGDF
                     EMAPQAYFDLVTQAIDIAYAKTFGELIPATDELLAARIAGHQRSFAIDLVVAVLVILV
                     LAYLSGATYVSVNDAVTRLSNGAESLANGDLSTRIGLESRDELGDVAERFNAMAAAFS
                     EVIRKVQQGADAVAGSSVSLAGSAARVSDGSEQQSAAASSMAAAVEEMTVGIEEIAVN
                     AASAEGVSSESGKLSQEGGAVVARTVSEMERIADAVQESARVIQELGDQSAQISTIVN
                     SIKEIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTAKATEEITTMVASI
                     QAGTGKAVDTMQDGVRRVQEGVQLAGQAGISMDQIRSGAGRVVTAVTDISAALREQGV
                     ASTEIARNVERIAQMAEQNSAAVRDTAGTARELERLAQQLRGDVQRFRI"
     gene            complement(38159..39247)
                     /locus_tag="PLH85_04000"
     CDS             complement(38159..39247)
                     /locus_tag="PLH85_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNB63751.1"
                     /translation="MKLDEFVTAWQQPAPKRRIPMRQSRRSQLPRILLRIYFAAGVLL
                     GLIVLGTVGFYAAGGEQANLSDALYMTLITVTTVGYGEVVPIDGIAERLFAGLIALAG
                     FGAVTFLFTSLTVFFLESDLDYSLRRRRMEKQIRKLHGHFIICGYGRVGRNVGNEMTA
                     TNRHFVAIDCDEAGMESQRERSPGLVYLVGDASDDDLLMSAGIQDAQGVFAVTGDDSR
                     NLMIVFTAKQLNPSIRVVARCHEVRNAEKLRKAGADSVISPDFTGGMRIASSMVRPHV
                     VSFLDEMLRSEKKLRLEEVQVPPRFEPRDLGTLQLRSPNYVLLAVREGETFVFNPPKD
                     FRLQPGQHVIAMASPLGRQELETALLPH"
     gene            complement(39244..39777)
                     /locus_tag="PLH85_04005"
     CDS             complement(39244..39777)
                     /locus_tag="PLH85_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNB63752.1"
                     /translation="MRRLLKRHLPTPESLADHRWLAWWGAPLLHPRLWHLNRHSAAGA
                     VAVGLFCGLIPGPFQMLGAALCSVMFRVNLPLALFTTLYTNPFTIVPLYLAAFTLGQW
                     ILGPDGRTFIAPPDLDGSLLHWLGQLAHWAADLGRPLALGLVALAAVLACSGYVLTRL
                     AWRIHLVRAWRRRKGLA"
     gene            39901..42258
                     /locus_tag="PLH85_04010"
     CDS             39901..42258
                     /locus_tag="PLH85_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNB63753.1"
                     /translation="MSISGFLVGVVSCQFGAALPAPSCQFAAAVLAAGCGLAGALLYR
                     SSRERWPGLALVLSMLAGLAAGYAYAGWRAELRLAQSLPGDWEGRDVAVTGIVATLPQ
                     NFGRGVRFVFEVEAPAGGAVPRRISLVWYRGADDDEAGLLGEVHAGERWHLLVRLKRP
                     HGNVNRHGFDYEAWLLEQDLRATGYVRADASNRRLDAFVLSPGTLVERLREVVRSRFQ
                     AALPDGRYAGVLIALAIGDQRAIPQEQWSLFARTGIAHLVSISGLHVTMVAALCAAVI
                     GAIWRRVPALALRVPAQQAAVATGALAAGGYALLAGWGVPAQRTFAMLAWVALALLLR
                     REAAPFRVLGLALLGVLLLDPWAVLAPGFWLSFGAVALLFLAGSGRLAAGGRIREALR
                     AQWAVTVGMVPALLVLFQQCSLVSPLANGLAIPVVSFVVTPLALLFSVFPLQILADVA
                     HAVLGGVMLPVEWLGGLPYAQWQQAAPPFVLALMATLGCLWALLPAGTPARWLGASML
                     LPLLAWHPARPAPGALKMTVLDVGQGLAVHLQTAAHDLLYDAGPAFSADANSGERIVL
                     PYLRAQGVRRLDSVVVTHQDNDHAGGAEAVLGGIPVAEVRSSLPTAHPARLMAADRHL
                     PCRAGDAWEWDGVRFRVLHPGAEADADRPRKTNNLSCVIRVDAAGHSVLLTSDIEASD
                     EEALLARDPGALASDLVLAPHHGSRTSSTPAFVAATHPAWVLFPVGYRNRFHHPNPEV
                     WSRWSGAGARMARTDAEGALEVRVDAEGVQVEGARAVAPRYWYGR"
     gene            42371..43312
                     /locus_tag="PLH85_04015"
     CDS             42371..43312
                     /locus_tag="PLH85_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB63754.1"
                     /translation="MSLIDALRRLFTPAPVVVPPAAEVSAGGFEQPRFHRVQCAGPHG
                     LHRIAYTEWGDPANPRVLMCVHGLTRNGRDFDDLARSLAHHYRVVCPDVVGRGQSEWL
                     AVKDDYQLPTYVADMITLIARLNVETVDWVGTSMGGLIGMLVASLPEQPIRRLVLNDV
                     GPVITVSSLRRIGEYVGKAPVFPSYEAGEAFIRAVSEPFGRLSDAQWRQLAVHSLKQT
                     EAGWEMRYDPGIGEPFRKTPVLMDVELWSIYDAIACPTLVVRGAESDLLLRETAVEMG
                     RRGPKASLFEVPHVGHAPTLMEPAQIAPIRDFLLGSR"
     gene            43312..43998
                     /locus_tag="PLH85_04020"
     CDS             43312..43998
                     /locus_tag="PLH85_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HNB63755.1"
                     /translation="MLATLVSAFSVVDWAALAWFFACWMGYGWVVEHGPKGGMGGLLG
                     ASHRFRLEWGRQMLRREVRVADSALVGNLMQNVSFYANTTIYVIAGLLALLGTVDKLI
                     DAAAELPFARHVSREVWEVKVLLLLMVFVVAYFKFTWSLRQFNMLSILIGAAPPPGEG
                     SEADAQRFAKVNALAGDEFNRGIRAYYFGLAAVAWFVQPWLFMAVTTFIVLVLYRRDF
                     ASHTLAAMRE"
     gene            complement(43999..44397)
                     /gene="msrB"
                     /locus_tag="PLH85_04025"
     CDS             complement(43999..44397)
                     /gene="msrB"
                     /locus_tag="PLH85_04025"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNB63756.1"
                     /translation="MSRKIEKTDAEWRAQLSPEEYHVTRQKGTERAFTGRYWDCHDAG
                     EYRCVCCGAELFASEHKFDSGCGWPSFHSAAAPGNVEEAVDRSHFMVRTEVLCRQCGA
                     HLGHVFDDGPAPTGLRYCINSAAVKLKPKE"
     gene            complement(44466..45635)
                     /locus_tag="PLH85_04030"
     CDS             complement(44466..45635)
                     /locus_tag="PLH85_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HNB63757.1"
                     /translation="MKRNRLGRRGGLPRDAEQLLWLASGLAESSSRAEDHFWDQRLAA
                     AVDGLLRAEDEDTLNTALDHLSSTNAAAYDELADMIESRAEGALTSDDPHDVLLIAAP
                     VMAWSRYRIPSATIPAAALANLRVQLKAHVLAKDVKLALADYLFSPDQLPQGYCATAD
                     FASHLARSAATDADLRIKTDNLPETAQFLSDTRYILGAVVVPKGAPIFRWQEEDCTRD
                     QAFEQWRAQGAACIAPLLTGCATEVVLPNAYFAASREADKASRPYSIQASVAFLSTTL
                     DAPATRLRAVVAPFFERQVEEFRIGFTLRNSNDVVHGVVWPLLGAEGETSETLGEIEA
                     TLRACGITDIQVLDNEFPMEYCDDCGAPMYPSPEGEAVHAELPEEQAEQMPKHLH"
     gene            complement(45712..46536)
                     /locus_tag="PLH85_04035"
     CDS             complement(45712..46536)
                     /locus_tag="PLH85_04035"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNB63758.1"
                     /translation="MSPTRPVFFISDGTGITAETLGHSLLAQFPDTRFRARRLPFIDS
                     LEKARDCALLIREAASETGLRPIVFNTLVDPAAVAALRESDALFLDLFEKFIGPLESE
                     LGQRSTHTVGRFHGIAESQDYARRIEAINFTLAHDDGVSHADLQEADVILVGVSRSGK
                     TPTSLYLAMQFGVKAANYPLIPEDFERNKLPGELARHRHKLFGLTIGPDRLSQIRQER
                     RPNSRYASIENCLGEIEAAQRMMKREGIRWLDSTAKSIEEISTIILQEVRLNPSSF"
     gene            46764..49133
                     /gene="ppsA"
                     /locus_tag="PLH85_04040"
     CDS             46764..49133
                     /gene="ppsA"
                     /locus_tag="PLH85_04040"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNB63759.1"
                     /translation="MVRKVIPFQELRMSDVEQVGGKNASLGEMISQLPASVRVPGGFA
                     TTAAAYREFLSHQGLADRISAALATLDVDDVVALAKAGAQIRQWIVDTPFPAALEAEI
                     KAAYEQLTTEGDGSFAVRSSATAEDLPDASFAGQQESFLNIHGYENILHAIKEVFASL
                     YNDRAIAYRVHKGFDHAGVALSAGVQRMVRSDIGASGVMFTMDTESGFNGVVFITASY
                     GLGETVVQGAVNPDEFYVHKATLALGKPAVIRRNLGSKLIKMVFADKKEAGKSTRTVD
                     VAEAERNVYSLTDEDVLELARYATIIEQHYGRPMDIEWGKSGDDGKLYILQARPETVK
                     SQQSGHVMEKYRLKQYGKSLTHGRAIGQKIGAGPVRVVKDASEMERVKAGDVLVTDMT
                     DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAIVGCGNATEVLDENDEVTVSCA
                     EGDTGYVYRGKLDFEVVTTDMGNLPDIPVKIMMNVGNPELAFEFAQIPNGGVGLARLE
                     FVINNMIGIHPKAILEINQVPASLRNEINRRSRGYATPRQFFVEKLVEGVATIAAAFY
                     PKPVIVRLSDFKSNEYRKLLGGEIYEPEEENPMLGFRGASRYIAHSFRDCFELECAAM
                     KKVRNELGLTNVQLMVPFVRNLDEAAGVVDLLARHGLKQGENDLKLIMMCEIPSNALL
                     ADQFLQYFDGFSIGSNDLTQLTLGLDRDSGLVAHSFDERDPAVKQLLSMAIQSANKLG
                     KYVGICGQGPSDHADFAEWLMDEGIQTISLNPDTVVDTWLKLAAHSNKG"
BASE COUNT         8431 a        14561 c        17177 g         9138 t
ORIGIN      
        1 actccaacga agtcgccgcc ctggggctga agaagaagtc ggagcacaaa ccgcgatcgc
       61 gggggccgaa gggggagaag tagggagacg cggcgaatga gcgggaggtt gttttcgtgg
      121 atagccttgt ttgctacggt acgctgatgt gaagacgttt ctactcagtt ttggggatgc
      181 gtggagataa tgattaaggc acgatgcgcg gtgaatataa aaccgtatcg gttttgatgc
      241 tgcgatttaa cgggctttgt ttttgatatg tatggagaag cgttgctctt ggggtagttt
      301 aaatttattc atgtttatta aatttgaaga aaatcgcaag ctcgaaagca tagatctgtg
      361 caaattcttt tttttcagat ctgcttttgc agcgtatgtc gtttgcgcgt cgtcgtacgt
      421 gtttgcgcaa gttgcttaca tggagccgac cgtgccgatt gtcacgacgc catggaaaga
      481 tggctggatt acggcgggtg gtcctcaaga gatttgccag caaatattca ttgaggctgc
      541 tggaaatcct ccggagcgac cgattacaat ggtgttgtcc tctcttagga ttataccgga
      601 acctaatcac aattacagcg tcagcgattt tagacttggt gaggtgcttc cttacgatac
      661 gccgaatttt tccgcttggt attgctcggc atcctatgat cattacgatt atggtggtct
      721 tcaccaccac aatggagatc ttggaaattc gggggagttt ttataccctg tttgccctaa
      781 tcatccatac cccggtacat atccgttggg agaaccaaat ccgccaaagt attgtaccgg
      841 tccgacaacg cccgttaggc cgccggtttg caatgagatt gggtatccag tcgagcccgc
      901 tagtggtgta aaagtattta gagagcttga ctttcgtcac tcctcaaatc cccatctata
      961 tctggagaga acgtaccgat cgttctttcg gatacagcct gcaggaatgg tctcctggat
     1021 cacaaactgg catcgcagac ttttctttca ggaaattgag aaggacgtca gtgtagtgtc
     1081 ggtagatgag tccgggcgtt cgcgatattt ttatacgagt caacttgttc cggagcatgg
     1141 gcttttagat cgtcttacta agacatcaag cggctatcag ctatttcggg ccgacaacac
     1201 gctggagcaa tatacgtctg atgggtattt ggcgggtgtg tatcatcacg atggagccta
     1261 tgaaatcatc cggtatgtcg gtgggagtgc ggttgggtcg tggaatgttg cttccataac
     1321 agatcacaat ggggctgcta taaagtttaa ctacgatgcc gtggataggg tgtcatctgt
     1381 tacgacttct gaccaacgag tgataaatta tttgtatttt aattacaatg gctacccgct
     1441 gtctgttgtt ggttggcagg atggaaaatc gaggtcgtac acatattctg tcgagtctcc
     1501 ttcttcgtgg atgaatatct tgtctgttgt tgatgaggat ggtgcgctgt tttcgcgggt
     1561 tgaatatgat cgcaacaata gggtcgtgta ctctggaaga gggggtgatc agagtagcta
     1621 cacctttaag tattccgaag ataaatggtt cccgacgtcg actgttgtca ccgatccatc
     1681 gggagttcaa cgtacctata gctcggtcct ggacaaatcc cagatatttc cgaccggagt
     1741 atcgaagccg gggggcgccg gctgcaacgc atccagcagc aatattgact acgatacttt
     1801 cggtaatgcc atctcccgca cggatttcaa cgggatgaag tcctgccatg gttacgatgc
     1861 ttcccgtaac ctcgagacct cgcggatcga gggtttcgcg tcgtccgatg catgtccggc
     1921 cgatgtgctg tcttcggcgc cttcctccac acagcgcaaa accaccacgc agtggcatcc
     1981 gttctggcgt ctcgaaaccc ggcaggccga acccaagcgc gtcaccacct gggtctataa
     2041 cgggcaaccc gatcccacca atggcaacgc gctggcctca tgcgcgcccg ccacggccaa
     2101 gctgccggat ggtcaagtca ttgcagtgct gtgcaagcgc atcgaacagg ccaccactga
     2161 cgcgactggc gcgcaaggct tcggtgcgtc cgccgatggg ccggcacgga tctgggccta
     2221 tacctataac gcgttcggac aggtgctgac cgaagacggt ccccgcacag acctcgcgga
     2281 cgtgaccaag tacgtttatt acaccgacac caccgccaac tggactcaag gcgatctgcg
     2341 cagcattacg aacgctctgg gccatgtgtg gacctttacc cagtacgaca aggccggccg
     2401 cctcctggca gcaacggacc ccaatggcac cgcgttgact tatacctata ccccgcgagg
     2461 atggctggcc agtcgcagcg tggcgggcca gaccagcacc tttgcctata cgggatgggg
     2521 tgggctcgcc ctggtcacgc tgccggatgg cacgtcaacc agctacacct gggatgccgc
     2581 tcaccggctg accagtgttt ccgacagcgc aggcaacagc acgcgcttcg tgctcgaccc
     2641 gtccggcaac atcacttctt cgaccaccac ggacgctgcg ggcaatgtcg ctcgccagac
     2701 acggacggag ttcgatgcgc tcgggcgccc ctggaagcac tatgacgcag ccggccatgc
     2761 cttcgaggtc tggcacgacg cgatggatcg ggttgccaag accattgatc ccaaagggcg
     2821 gaccacgacc tttgcacgcg acacgctggg acggcttcag gacatcaccg atgctcagac
     2881 cacgcccggt cacaccggct tcgcctttga cgcgcgggac caaatgaccc ggcaaaccgc
     2941 ccccaacggc gccgtgaccc aactgagcgt cgatggcctc ggaaacaccc tccaggaggt
     3001 cagtgccaat cgcggcaccc tgcgtttcac ctacgacgcg gcgggcaacc tgctgacacg
     3061 taccgatgcg cggggcgtca ctgcgaacta cacctacgat gcgctcaacc gcatccgcaa
     3121 gcgcatcacc aagaacagcg caggtgttgt gaccgaaacg ctcgactaca tctgggatgg
     3181 ggcatccggc tgtacctacg gacgcggtca cctttgccag atcaccgatg gcggcggggt
     3241 gacgaacttt gcctacgatg cgcggggcaa tcggatcagc gagcgccgca gcgaagccgg
     3301 tagaagctac acgactcaat acacactcaa tggcgcagac cgcatcgttg ccctggtgac
     3361 gccgaccgga tccctgctcg atcaggcgcg ggacgccggc gggcgtgttg ccgcgctgac
     3421 ggccaccgcc ggtggttcat cgattcccgt ggcatccgca gtccgctacg acggggccgg
     3481 aaacgtcgtg agtctgagcc agggcaccag tctgtcccaa ctcatgcagc gcaacgccga
     3541 cggacaactg gaggcgaacg ggctgggcat cccggtcacc ggcctggggc tgagcctggg
     3601 caaacgcttc atccgtccgg gggatgggct tgacttcacc ctgaccctga cgcctgccca
     3661 ggcctcgggg accgtgttct tgtgccaact cgattgccag ggcagcaacc aactggcggt
     3721 cattcccgta acggggggaa caacgacggg aacgctcacc ggcctgacca aaggcgtaca
     3781 ccacctgagc gccgtactcg tgcccaaccc tccttacgct gccagcctgt ccgagtcgcg
     3841 ccgcgtactc gtcggcgtac cgccgaccct cttcttcgac gatctcttcc tgcagtaacg
     3901 cccgaggacc gcattcatga accgtcgcgc cctgatcctg accctgatga tgacattacc
     3961 cgccctgcct gccgcagcgg ggacgctggc ctgcaacggc acgattgcgc aagtgatgac
     4021 gaccgcaagc ggtgatctgc gcgtcgtgcc cagctggcgc aatgactggg tcaccctgtg
     4081 caacgtcgta agcccctgga aaggcgtccc ggtggacgcc tgcaaacgct ggcatgccca
     4141 agccctggct gcccagatcg cccagactaa cacccagacg gcctatgcca gcacggcagc
     4201 cacggcgtgc gctcagatgg cggtcgcctc agcggccgac gcccccgata ccttcagcaa
     4261 caactgaggc ccggcagaca tgttccgctc cattctgaga cttgcattgc tgtgcggcac
     4321 cctctccagc cccgtcgccc agtctgctgc actgtcctgt acaggcaccc tgaccgatgt
     4381 cgcgaactcg gctgcaggcg aactgcggat caaaccctcc tggcgggggg actggatcac
     4441 cctgtgcagt actgcggtcg tattgaacgg ggtccagacc gaagcgtgca agcgctggca
     4501 ggcccaagcc cttgccgcgc aactcacggc tgccaacacc tgggttcaat acgacaaccc
     4561 ggtcgcgccg agttgcgcaa cgatggggac actcggcaac gcagaccgtc ccggctatct
     4621 ggtgacgtac tgacgaggcg cctcgagatg actggtcttt ccacgttgcg cgcatgcctg
     4681 ctgggcacga gctgcctatt ggcggcgagc gccgctcacg cagacaccta caactatgac
     4741 gagttgggaa acgttcgaca gatccagaac gacaaaggcg tccggaccta cacctttgac
     4801 gaggtgaatc gtctcaagac cgagagcgga ttggccgggg tgcgcaccca tacctacgat
     4861 cccaacagca accgcctgaa cgatggcgcc ccaagcccga ccacggccag cttcgccccc
     4921 gcatcggacc ggatcgcaac cctcaacggt gtgaccgtta ctctcgacgc cgcaggcaat
     4981 ctgctgggcg acggcaccta cacctacgcc tggaacgacg cgggacaact gctctcggtg
     5041 agcaaggccg ggcaactggt cgccaagtac tactacgatt acaagggacg ccgcacgcgc
     5101 aaggccacca ccgccatcgc cccgcaaggc gcccaggtgt tcgtgtatca ctacgacgcc
     5161 tccgaccacc tgatcggcga gaccttgggc gatgcgacgc cgttgcgcac ttacgtttgg
     5221 ctgaacgatc aacccaacgc agtgatcgaa catccggcgc tgagcacccc gcagcgcacg
     5281 ctgtaccttc aggtggatca cctgggcacg ccccgcctgg ccaaggacag cacgggcaag
     5341 atccaatggc gctgggacag cgacggctac ggaagcaccg cggccgacga ggatccggac
     5401 ggggacggaa agaagacgac gatcaacctg cggtttgcgg ggcagtacta cgatgtggag
     5461 tcggggctgc actacaactg gaacaggtac tatagtccga ggctcgggcg gtacatttcc
     5521 agtgatccga taggggtgga gggagggggc aatacctatg ggtatgtcgg cgggaatcct
     5581 ctctcttatt tcgacgtgct cggattgtcg ccgatgtcgc ctacggagat tgcccaaatc
     5641 attaaggaca acaatcactc gccgctgagc aatgagatgg ttatgtgcct gatgtggcaa
     5701 gagtcagggt ttgacccgga tgttgaaaat gatggtgcac gtggcttaat gcaaattcgg
     5761 aagaatcagg cgctggcgca agtaaacaag aagagtggac ctttcgcttg gcaagacttg
     5821 tttgaccctt caacaaacac tgaggtcgga acgctctatc tcaattgggt ctctcaacga
     5881 ccgggggact tagatagaaa tatccagaga agatacggta ctggagatga ttatcctgtc
     5941 gcaaagatgc ggcaatgcga gaagtgtcta caggaaacgt gctcaaaaga tggggatgta
     6001 aaaaaatgct tagcaaaagt gcatcgttga tttttagact gctatttgtt gttgcagcct
     6061 tgggggctca atgctctttt gcaaacgatg cttgcagcag tttttctgca gcggatataa
     6121 ccacctacct tccggacggc gagggatatt ggatgcgcgg acgcaatggc ccaaatggtt
     6181 ctgcaattag tgtctgtacc gaaagaggaa aaggggtgcc tgtcgccttt ataagtgaag
     6241 acagcgcaca agagtttgct gacttgcaca ttctcagcat tcaatcgttt tatgggctgc
     6301 ttatcgttac acaagaatct ggcgccgctt atcggattcg tttttttgcg tatgacatta
     6361 aggcgaacca agtaagagag atggcaagtg atggttcaaa gtttttgcct tcactgggct
     6421 accttgggtc agaacttcaa ccggtggttg gggtcatcca ttctgcgcta gattcgagag
     6481 tttcttctca gccgctccct aaggaagaag tcctatattt tctcgacaag agcaagatga
     6541 atatctcgtc taaacgcaag gcatggagtg cgaagcgcta agccggaaaa aacaggcaaa
     6601 acatctatgg ctatgtcggc gggaatccgc tgagctatac ggacccaacg ggggaaatca
     6661 aacgtccaaa tgacccgagt ggcctaccta gcgattggca acgtgatact agacaccgcg
     6721 accctaacgg cgagagatgg acaaaccaaa atggggatgt tcttgattgg caccaaggtc
     6781 ggcctggtat gcctggctgg agaggtaaag accattggca ccacaatgga ggagatgagc
     6841 atttgcctcc tgggactgaa gtgccggatt caccgagcga cggaaaaaca tgcggggatg
     6901 attgcaaaca gaaggttgca acggttgtca cgtcagcgac tggcgcttac atcatctatc
     6961 gttgcgtacg tatgattcca tcacttgttc ctccgctttg gtggaccatc ccgggtaatg
     7021 ctgctgcgcc atgaggtaat tatgaatagc tttaatgatt ttcttttgcg aatggggggt
     7081 atgcacgact ctatcgttga gcgccttatg tggtcgccag acgaaaaacg aattgaactt
     7141 caatttgatg atttgtatcg taacttcaat ggattgccag agtaccctgg caggcagccc
     7201 ggagttatag ttttacatgg tgttagcaat ctgagtctta cactcgattc tgacggacct
     7261 ctgcgggtat tcgagttttt gcccgaagaa ggcgagtcag atgttgtagt tttgactttt
     7321 tcgccaacgg gaaagctccg cgtccaatat aggtccgttg accatccagc atgcctgatt
     7381 aatgcagtgg gccgctcttg acaaaccgcc cctgctcatc ctggtgatct agcctatgaa
     7441 cagaccgttc ccccgcttcg acacgacgcg ccgccctggc gtgttgttcg tctcagcagt
     7501 cgctcagata ccgtcgcacg cgtaagcgcc gtgcgggtat cgctctaatt cccttcctaa
     7561 gtcgatagcg cctgccggcc ttgcgcgtgc cgtttgccgc gtgcggacgg gtgtccgtgc
     7621 gcgtttcaac cgaaatgcaa aggatgcgac atgagcaaga tgaacgagta ctacgaagaa
     7681 acccgcgcca gctacctggc tgccgtcatc ggtggcatcg aaaccggctg catcagccag
     7741 caggacatgg aagtgctgca cgtacctaca catccgcctc caccgaatcc cccttgtctt
     7801 ccatccacgc gcggcggccg gcggcttcgc ctttgcccat gagcatggtg aaggtgcggc
     7861 gggtttcttc cagggcggcg gggcggacct gcacggggag gacgcggcgg gtggcggggt
     7921 ccatggtggt ttcgcggagt tgttcggggt tcatttcgcc gaggcctttg aagcggccga
     7981 cttcgatgtg ttcggccttg acgccttcgt ggaccaggcg gtcgcggagg gtggcgagtt
     8041 cccggtcgtc gagggcgtag agccggcgcg gcgggcgctt tttgccctgg gcggggacgt
     8101 cgatgcggta gagcggcggc tgggcgacgt agatgtggcc gtgctcgatg agctggggga
     8161 agtggcggaa gaacagggtg agcagcaggg tctggatgtg ggagccgtcc acgtcggcgt
     8221 cggacatgat gatgactttg ccgtagcgca gctcggagag gtcggcggtg tcgtcgaggg
     8281 cgtggggttc gacgccgagg gcgacggcga tgtcgtggat ttcggcgttg gcatagaggc
     8341 ggtcgcgatc cacttcccag gcgttctgta ccttgccgcg caggggcagg atggcttgcg
     8401 tgtctttgtc gcgggccatc ttggcgctgc cgccagcgga gtcgccctcc acaaggaaga
     8461 gttcgttgcg ggcgatgtcc tcggattcgc agtccgacag tttgccgggt agcacggcga
     8521 cgccggagga cttgcgcttt tccaccttct gggcggcgcg ggcgcgggcc tgggcggcct
     8581 tgatggccag ctcggcgagc ttcttgccgt actccacgtg ttcgttgagc cacagctcga
     8641 aggggtcgcg caccatgcgg gagacgagct gcacggcgtc gcggctgttg aggcgttcct
     8701 tggtctggcc ctggaagctg gggtcgagga cgcgggcgga gaggacgaag gaggcgcgcc
     8761 cgaaggcgtc gtcggcggtg agcttgacgc ctttggggag cagggcgtgg tggtcgatga
     8821 agttcttgac cgcggtgaag atgccgtcgc gcaggccggc ttcgtgggtg ccgccggcgg
     8881 gggtggggat gaggttgacg taggattcgc gcaccggcgc gccttcggcg gtccaggcca
     8941 cggcccaggc ggcgccttcg ccggcggcga aggtctcgtc gcccttgggg gcgtattttt
     9001 cgccggagaa caggggaatg aggggttcgc cggtgaggga ttcgttcagg tagccgcgca
     9061 tgccgtcgtc gaagcgccag ttgcgggtgt cgccgccttc tacggcgagg ctgacgtcca
     9121 ggccgggcat gagtacggcc ttggagcgga gcaggcgttc cagctcgccg cggggcagct
     9181 cggcggcgtc gaagtatttg gcgtccggcc atacgcggac ggtggtgccg ctggctttct
     9241 tgggggcgtc gccgatgcgg acgaggggct cgatcacgtc gccgccggca aacaccaggc
     9301 ggtgtcggcc gccgtcgcgc acgacttcca cttccagccg gcgggcgagg gcgttggtga
     9361 cggacacgcc gacgccgtgc aggccgccgg agaagcggta ggcggagcct tcgtcggtct
     9421 tgttgaactt gccgccggcg tggaggcggg tgaacaccac ttccaccgtg gagacgcgtt
     9481 ccaccgggtg gatctcgacg gggatgccgc gcccgtcgtc ggccacgctc accgagccgt
     9541 cggcgtgcag ggtgacgccg atgcgccggc agaagccggc catggcttcg tcggcggcgt
     9601 tgtcgatgac ttcctggatg acgtgcagcg ggtgctcggt gcgggtgtac atgcccggcc
     9661 gctgtttgac gggttcgagg cccttgagga cggtgatgga gtcggcggtg tagtgctttt
     9721 gggtcatgcg cggtccggcg ggtgggagtc tggagcgacg cggactatac cgcggggatc
     9781 gtcagggcgc gagggtgtgg cgtatgcgtg cgtgacgggt gcggcgcgcc tcagcgcggg
     9841 cgggcgcggc cggtgttgtc gcttacctgg gcggcgatgc gttccagcag ggcattggcg
     9901 gcgcggctgc cggcgctgtc gccctgggtg gggcgccagg ccagatacac cgtgcccgcc
     9961 tcgcctgcgc gggtgtagat ggccatggag agggggcatt gggcgatgtt gacggggttc
    10021 tcccgggcca gttgccaggc tacgcgggcg ctgcagaaat ggagcacttc ggcgtcgcca
    10081 tacacggcgc cgtcctgacc cagggccggg ccggtgcgtg ccagcatgtc gccgaagcgg
    10141 ctgggcggcg aagggatcag gccttcgcct tcgatggcgt ccaccagcgc ctcgcgggcg
    10201 tccgcgtaac gggcgtcggg gaggctgacg tggcgcagtt cgtccgcgta ggccgaggcg
    10261 gcgagcagag ccgcgagcag cagggcgagg caggcaaggc ggggcagggg gcgcatggtg
    10321 ggacgatagc aaatccgcgc gcgcgggggc agccgcgggc gctgcggacc gggtcgttca
    10381 gggccggttg tccggatgga tgcggcgcgc cagatcgatg aaggcgcgca ggccggcggg
    10441 cggcaggcgg tggctggggt agtagaggaa gaagcccggc tccggcgggc gccagtcgtc
    10501 cagcacgctg acgaggcggc cggcggcaat ggccggggcg gcgtattggg cgtagacgta
    10561 ggcgaggccc agccccgctt cggcggcggc gatcatgacg tgcatgtcgt cggtggcgag
    10621 ggggccatcc acttcgatct ccacctgctc gctgccgcgc cggaattccc agcggtaata
    10681 ctggccgccg ggaaagcgca cgcggatgca gctgtgggcg gtcagcgccc gcgggcagtc
    10741 cggcgtaccg cgggccgcca gataggctgg ggcggcgacg acgacgaagt gctgggcggc
    10801 cccgaggggc acggcgacca tgtcctgctg cacgctctcg ccgaagcgca cgccggcatc
    10861 gaaaccgcca gcgacgatgt ccacgaagcg gtcgtcggcg acgagttcca cgcgcatgcc
    10921 ggggtattcg gcgaggaagc ggctgaccag gggcgccagc acccacgcgc aggcggcgcg
    10981 gggcgcgttg atgcgcaggg tgccgagggg cgaatcgcgg aaggcattga cggcgtcgag
    11041 ggcgccgtga agctcccgca gggccggagc gacccggtcg aggaggcgct gaccggcttc
    11101 ggtgggcgcg acgctgcggg tggtgcggtt gagcaggcgc acgccgaggc gggcttccag
    11161 cgcgcgcagg gcgtgactca gggccgacgg ggagacgccc agctcggccg cggcgcggcg
    11221 gaagttgcgg gtccgggcaa cggtggcaaa ggtgtgcaga tcgttgaggc tggccagggg
    11281 cggagcgttc attgttgaga tgtgctcaat aagtcatgcg tcagtgtgcg gattgttgca
    11341 tggagcgcgg cccgttacgc tgatccggtc atccccaatc ttcgacagga gcccgcaatg
    11401 cagacaagca cacagtttcc cgatctggcc ggcaaggtca tcctcgtcac cggcgcctcg
    11461 tccggcatcg gcgccgccac ggccctggcg ctgggccgcg ccggcgcgcg ggtggcgctg
    11521 gcggcacgcc ggctggacag cctggaagcc ctcgccgcgc agatccgcga tgccggtggc
    11581 gaggcgctga cggtgcagac cgatgtgacc gtggaaagcc aggtacgcga tgcggtggcg
    11641 gcgacggtgg cgcgcttcgg ccgcctggac ggcgccttca acaacgcggg cgccctgggg
    11701 gccgccgcgc ccctgcacga gagcacgacg gacaacttcg acgcggtgtt caaggccaat
    11761 gtgtacagcg tgtattggtc gatgaaatac gagatcgccg cgctgctgca gacgggcggc
    11821 ggggccatcg tcaatacctc gtccatcgtg gcgcagatcg gctttcccgg cgtggcggcg
    11881 tacacggcca gcaagcacgc ggtgatgggc ctgacccgca acgcggcgct ggaatacttc
    11941 acccagggct tgcgcatcaa cgcggtgctg cccggcccca tcgagacgcc catggcggaa
    12001 tccggcttcg gcagcctgga ggcgctgcgg gagagcatga aggcgtcgcc cgccggccgc
    12061 gccggcgtgc ccgacgaggt ggccgcgccg gtgctgttcc tgctgtccga cggggcccgt
    12121 tacatgagcg gtcaggcgct gaccgtggat ggcggttaca cggtggcgtg aggcggcgtg
    12181 ccgtatcgtg atgggccggc ggggcgcgat ggtggcggcc cgccggcccg gcctcgcaat
    12241 gctttgaaac caggagtgcc tgcatgaacg ctgtgtccgt ttcctcaccc ctccgcagcc
    12301 tcgggccgct gcgggtgccg gtcgcgccca tcggcctggg ctgcatgggc atgagcgagt
    12361 tctacggccc gtccgacgac gcggagtcgc tggcgaccct ggcgcgggcc ctggaactgg
    12421 gcgtgaccct gttcgacacg gccgacacct acggccatgg cgccaatgaa cgtctgctcg
    12481 ggcgcttcat cgccgagggc ggtgctgcgc ggcgggcgcg catggtgatc gccaccaagt
    12541 tcggcatcgt ccgcgagccc ggccgctacg agcggcgcat cgacaattcg ccggcctacc
    12601 tccgtgccgc gtgcgaggct tcgctgcagc gcctgggcgt ggaggcgatc gacctgtatt
    12661 tctgccaccg gcgcgatccg gccgtgcccg tggaggacat gatgggcgcg ctggcagcgc
    12721 tggtgcaggc cggcaaggtg cgtcaggtgg gtctgtcgga ggtgtcggtg gacaccttgc
    12781 ggcgcgccca cgcggtgcat cccgtggcgg cagtgcagag cgagtattcc ctgtggagcc
    12841 gcgagccgga gcagggcatg ctcgccgcct gcgccgagct ggggacgaga ttcgtcgcct
    12901 atgcgcccct cgggcggtcc ttcctcaccg gcacgaccga cgtggcggcc ctggcggaca
    12961 acgatttccg caagcacaac ccgcgcttcg ccgccgaggc cgtcgagcgc aaccggcaac
    13021 tggtggaggc cctggcccgc tttgccgagg cgcgcggcgc gagcagtgcc cagatcgccc
    13081 tggcctggct gctggccaag cagccccacg tgatcccgat ccccggcacg cggcgcatcc
    13141 gctggctgga acagaacatg gcggcggccc gcatcgccct cgatgcggac gaggtggcgc
    13201 agctggacgc gctgttcgcg ccggagcagg tggtcggcgc gcgctacccg gaggcgggct
    13261 gggtgggcgt ggagcggggc tgacgcgtca ggccgggcgg cccgcgaacc acgcccgcag
    13321 ggaatcttcg atggcgccgt agggatagtc ccccaccggc tcgatgccag cctcccgcag
    13381 ccgcttggcg ggcccgtcgc ccaccttggc gcagaacagg gccgtacagt cggcgagggc
    13441 gcgcaggatc acctcccgct tgtcctcgtc gccgaagccg ccctggcaat agtgctcgac
    13501 gacccgcact tcctcgaagg caataccgtc gggaccaacg gcgtagatct tgaattcgtc
    13561 ggcatgaccg aaatgttcgt cgatctggac acggtttttc gtcgccacgg cgacgcggag
    13621 gggggcgatc atggcatgga ctcccgatag tcatggggtg tcgcaggtgc gacaaggtcg
    13681 ggagacatca agcaaaggcg gtgccaaaaa cgttcgaggt gggttcgtcg gcgtgttgac
    13741 gtcaaaacgg catcccctcg aggatgccgt ttctctaccc gtgggtccgt cacgatccgg
    13801 cgggttcgcc gtcggatcgt ttcggggggc gctttattcc atcgcctcgt acagcggcag
    13861 ggtcaggaat tccgggtagt ccggggtcag ggacatcttg tcgaagatga ccgcggcctg
    13921 gtcgtaggtg gcggtgtctt cgccctgggc ggtgacggtg gccttcacct tggccagttc
    13981 ctcggcgatc atcgggcgga ccagctcttc ggtgaccttg cggccgtcgt cgaggacgcc
    14041 cttcgggctg accacccact gccacacctg ggaacgggag atttcggcgg tggcggcgtc
    14101 ttccatcagg ttgtggatgg gcacgcagcc gttgccggcg agccaggagc cgaggtagtg
    14161 gatgccgacg ttgatgttgt tgcgcaggcc ggcttcggtg atcggggtgg tgggacggaa
    14221 atccagccac tgctcggggc cgaacttgcc ttccacctgc ttctcgaact ggttcggctt
    14281 gtcgcccagc accttgacga actcttccat ggcgatggag acgaggcccg ggtgcgccac
    14341 ccagccgccg tcgaagccgt cgttggcgtc gcgggtcttg tcgtggcgga tgccggccag
    14401 ggccttttcg ttggcttccg gatcgccctt gatggggatc agggccgaca tgccgcccat
    14461 ggccggggcg ccgcgcttgt ggcaggcctg caccagggcc agggcgtagc cgcgcatgaa
    14521 gggcacttcc atggtgatgg cgccgcgctg ggccaggcag aaatccttgt tcttcttgaa
    14581 cttcttgatg caggagaaga tgtagtccca gcgcccggcg ttgaggccgg cggagtggtt
    14641 gcgcagttcg tagaggattt cttccatctc gaaggtggcg aggatggttt cgaccagcac
    14701 ggtggccttg atggtgccct gcgggatgcc gacgtggtcc tgggccatca cgaagatgtc
    14761 gttccacagg cgggcttcca gatgggattc catcttgggc aggtagtaga aggggccggc
    14821 gccgcgggcg atctgttcct tggcgttgtg gaagaagacc agggcaaagt cgaagatgcc
    14881 accgccgacg cgctgaccat cgaccagcac gtgcttctcg tccaggtgcc agccgcgggg
    14941 acggatctgc agggtggcga tcttgtcgtt gagcttgtat tccttgccgg cttcgttctt
    15001 gaaggacagc tcgcggcgga tggccttgta gaggttcacc tggccctgga gctggttgtc
    15061 ccagttcggg gagttggagt cctcgaagtc ggtcatgtag ctgtcagcgc cggagttgaa
    15121 ggcgttgatg atcatcttgg cctcgaccgg gccggtgatc tcgacgcgac gacactgcag
    15181 cgcagcgggc accggggcga ccttccagtc gccttcacgg acatgccggg tttcgggcag
    15241 gaagtcgggc atttcaccgg cgtcgatgcg ggcctgacgt tcgacacggg ccttgagcag
    15301 ctcctggcgg cggggctcga aggcgcggtg cagcttggcg accagttcga gggcttccgg
    15361 ggtgaggatg gtttcgtagc ccgggcggat cggggcattg atctgcatgc cggcggggag
    15421 gctcagttgg atgctcatgg tgactcctga tgaaaaaacg gtgagggatt gaaaggtgtt
    15481 tcggtccggg cggctcaggc ccggtggcgg gcgacgtaat cgaccacgtc gctgagctgg
    15541 cggccccggg cgacgggact gacgtcgagc tgctcctggg gcaggccgct gcggttgatc
    15601 cagaaggtgg tgtagccata ccaggtggcg ccggccgcgt cccagccatt ggacgagacg
    15661 aacaggatac gctccgccgg gcagccgaag gcggcggggc cgagggcata ggcgtcggcg
    15721 ctggtcttgt actgctggac ggcgtcgacg gacagcacgt ggtcgaagag gccgttcatg
    15781 ccggcgctct tgatggccac gtcgagcatc tcgggcgtgc cgttggacag gacggcggtg
    15841 ggcacgccgg cttccttgag ggccttgagg gcgccgagat tctccggaaa gggcgacagg
    15901 caggcgtact ggttcatcag ctgggtgcgc cgcggggcgt ccagttccag gccgaggcgg
    15961 gccgccgcat acacgaggcc gtcttcggtg acggaccaga agggcttgta gcggccggag
    16021 agggtgcgga tgaagctgta ttcgatctgc ttctggcgcc acaggtcggc cagctgggcg
    16081 cccttgccgg ggtagagctg ctcggcgagc aggccgacgg agtacacgtc gaagagcgtg
    16141 ccaaaggcgt cgaaggcaac tgcctggatc cggggagcgt gggccatgga ttgttccggt
    16201 gaaacgatgt cgggttgggc ccgcggggac gcttctgggc cggttcgtgg ccggctggca
    16261 tcctccgggg cggtgtctcc tcaccctgaa tggcgtgcat cttattcaag catcaaacaa
    16321 aaaagaagcc ataatgcgcg caaatgattt tttacttgga gtatcgaatg agcaacttca
    16381 aggagatcga agccttcgtc agcgtggctg cgcggggcag cttgtcggcc gcggcccggg
    16441 cggacggggt gacgccggcg atgatggggc ggcgcatcga cgcgctggag gaacgtctgg
    16501 gggtgaagct gctggtgcgc accacccgca agctgagcct gactttcgaa ggcagcgcgt
    16561 ttctggagga ctgccagcgc attctcaacg atctggccaa cgcggaggcg tcggtgagcc
    16621 tgggcggggt gaaggccagc gggcatatca aggtgtcggc gccagcgggc ttcgggcggc
    16681 gacatgtggc gccgctggtg agggatttcc tggcggccca cggggacgtg agctgcagcc
    16741 tggacctgag cgaccgcctg gtggacctgg tgaacgaggg cgtcgattgc gcgatccgca
    16801 tcggggatct gagcgattcg agcctggtgt cggtgcgtct ggcggacaac cgccgggtgg
    16861 tggtggcaag cccggcctat ctggcgctgc acggcgtgcc ggccacgccg gaggatctgc
    16921 tgagtcacga ctgtctgtcg ctcaatccgc agcgcggctg ggtgttctgc gatccggacg
    16981 cgccgggcac gacgcgggtg gtgaaggtgt ccgggcgctt cgaatgcaac gacggtacgg
    17041 tgctccacga gtgggcgctg gcgggcatcg gcctggcctg gcgttccatg tgggaggtgc
    17101 agcgggatct ggcgcagggc gcgctggtgt cggtactgga tgcctttgcg gcgccgagca
    17161 ccggtatcta cgcggtcttt ccgcagaacc ggcgcctgcc gctgcgggtg cgcctgctca
    17221 tcgaccacct gcggggcagc tttggtcagc cagactactg gcgggcgggc tgagtcggtg
    17281 acgattcggt gggtgtttct ggcggcggcg ggttgagttc gaactcgatg cgcaggcgaa
    17341 ccggcaccgg gtgcttgcgg tattcaccgg gggtgaattc ccaggtgcgc acgatggctg
    17401 cgtaggcgtc cgccgcggca cacagggctt cgtcgcactg ggtgttgacc gccgccacct
    17461 tgccgtcggc cgccacgatg acctccagct ggaaaatgcg atggccgagt tccgggaagg
    17521 ggggccagat ggaggcgggc accggcgtga gcagatcggg cggcgagctg agggcggaga
    17581 ggggcagggc gtccgccgaa gggggcgtgt cgtccgcgcc tggcgtgggg ggctgctcga
    17641 cgggcggggg ctcgggcggg tcggccgggg cgggcgcaag agagagcgcg tcatcccttc
    17701 gttcagcgat ggccggcgtg aatgtgacct ggaccacggc aggcgttcgc cccgccgggc
    17761 cggggcctcc aaaatacgtc gatgccgccg ttatcacggc tacgtggagc acaaccgaca
    17821 ggctgccggc caggcagagg ctccggggaa acgacgtgtg catggctgaa cggggcgggg
    17881 caaggctgtt acccggtagg cgtgaacgat agaggcacta tgtcacggcg ctgttgccgc
    17941 ctgtcggtga ccggaatccg tcggtcgcat tggactcgcg tttgaaatct gtcggtgtca
    18001 tctttcgctc gaaggagagc gtgcaatgaa gcgtcaatcg ggtgtgaccc tgatcgagtt
    18061 gttgatcgtc gtggtgatcg tcgccattct gggcggcatc gccgtgccgg cctacacgga
    18121 ccacatactg cggggcaaga tcgcgcaggt caccgagaat ctggccgagg ccaaggtgag
    18181 gatggagcag gtcttcaatt ccaaccggac ctacgcgact tcgccgggcg ggacgagctg
    18241 cccggatttc agcaccctgt tcgacaagac cggatttacc ctgaagatga gcgattgtcc
    18301 gggagatgcg ggaacgacct tcaccctgac ggcgacgggc agttcgagca gcggtacggg
    18361 tggattcatg tattccatca accagaacgg cgagaagaag tcgacgacgc cggccacctc
    18421 cgaggtgcaa tcctgctggt tgatgtccaa gacggcgtct tcatgttgat tccggcccgg
    18481 cgcctgcggg gcgccaccct tcttgagctg atggtcacgg tgtccatcgt ggccatcctg
    18541 atggcagtgg gtatgccgtc gatgaaggac tggattctcc gcaactcggt cagcagtgcc
    18601 gccgaagttt tgcagaacgg tctgcgccgc gccgagagcg aggcgatccg gcgcaatgcg
    18661 cccatcgagt tcctgctcac cagcggaatt ccctcgtcgt cggcatccct gacggccgag
    18721 gccaacgcgg tcaactgggc gatccgtgtg gtggacagca gcaacagcta caagccggtg
    18781 gcgggcgggt atgtggcagg tttctatctg aaggacatct cgtcggcggt aacggtcagt
    18841 ggcccggcca gcgtgatctt caacggcatg gcacgggttc tggatagcgc gggagtgccg
    18901 gtgaacgggt atcaggtgta tcgcttctcg cgaagcggct ccgacaaggc ttactgcgtc
    18961 tttgtgtccc agggaggagg cgtcaagatg tgcgatccgg atcaggcgtc gggcacgccg
    19021 cgggcgtgcc agcctctgtt gagcgcatcc cagtgcccga aggcggggag tggatcgtga
    19081 agcgtcggtc ctttgcaggc tatgtgttgc tggaggccct ggtggggctg ctgatctttt
    19141 cgctgggtct gctcggcctg atcggatttc aggcttcagc catgaagatc gcgtcagaca
    19201 gccgtttccg gacggaggcg gcgatgctgg cagatgaact gattggcaag atgtccatgt
    19261 cgaagatttc gaccgtgcag accgaatatt ccgcggacgg ctccaagttt tccgactggc
    19321 tcaacaatcg cgtcaagggc ggcagcaagt tgccgggtgc cgcagcgagt gtgaccttcg
    19381 ccacggccaa taccaatgcc accttgctcg caaccgtgac gatctcgtgg gatcttccgg
    19441 gttcaagcgg cagggtgggt gactcgctgc acggggttta tgtgacccag gccatgcttt
    19501 tctgaggaat cagcatcatg cgtcgcccgc cctttcccgt tctccgcgac cgctcgaggc
    19561 agcggggtat cagtctggtg gagacgatga tcggcatggc cctggggctg gtggcgacct
    19621 tcgtgatcat ccagtcgttt tcgtccagcg agacatatcg ccggaatctt gccggaacgg
    19681 ccgatgcaac ccagaccgcg gccattgccg gcggacatct ggatctcgtg ctgcaggagg
    19741 cgggcgcctc cctcgtgcag gggcggaacg tatgggggtg caagatcctg gtgacccgca
    19801 acaagaccgc catcctgccc catggctcca attatccgag tccgtttgcg agctttccca
    19861 ccacggtacg ggtgctcccg ctggccgtgc tcgacggcgg ggacaagggg tccgacgtga
    19921 tcatggccat ggtgggtaat tccggaaccg gcaatcgcga taccccgttc agtgcgccgg
    19981 cgggtggaaa gtccatggtg gtgtcgaaca ataacggcat ggcgctgacc tcatccggtc
    20041 agtcaccgaa cgatctcttc ctgtcggtgc cgcaggaggt ggcgggggcg ccgggggact
    20101 gtcaggtcgt gcaggcggac agcagcttct ccaacggcac ggcggttgcc gatccgtccc
    20161 tggggcttca ggtgatgccg gcgtcggtga cggcgcccca gtccctgacg accatcccgc
    20221 tcaatgcgtc aacctacggg gcgctggcgt cttccgccaa ttcgccttcg gccttccacc
    20281 tcgggcggga gtccgcgccg atcttttccc tgttcgcggt caacagcaac ggcgaacttg
    20341 tcgagtacga cctgctggaa cggaacgggg tcatgacctt cggtgagaac atcttcctca
    20401 tcaaggcccg ttatggactc gacaatgggg ccaacggagg caccgccaac gacaacgtga
    20461 tcgacgactg ggtttcgccg gccgaagacg gctggaagct tgcccagttg atggatggca
    20521 agagttcgac cgagcagaag gtcgatcaga tcaaggcggt ccgtctggcc ctggtgacgc
    20581 ggtcatccca gtcggtgacc tcggatgcca aggtgagctc cctgacactc ttcagcgacc
    20641 tctcgtccac gcggcaatac acccgctccc tgagcgactc ggaacaggcc tatcagtacc
    20701 agatcgtgga ctgggtgatt cctctgcgga acatgaaggc gacgcccaag taacggatgc
    20761 ggccatgacg acttccaaga ttcctcttca tccaccccgc cgcaatcgcg gagccgtgct
    20821 ggcgatcgtg ctcgtcgtgc tggtggtgat gatgctcggt gccgtgtcca tgcttcggtc
    20881 cgtcgatacg tcagccttgc tgtcggggaa cctggcattc aagcgggagt ccctgaatcg
    20941 atccacggtg ggcctcaata ccgcctttgc gcaattcaag aaggacgatt tcgtcaagta
    21001 ttcggacgac agcaccgggt gcaatgccgc gtcgaccggg ccgtcgtgca cctacgccag
    21061 caattggctg aagatgaatt attcgccgcg gctgcttgcg acggatggga acggtgtgcc
    21121 gcttatcctc aaggacatca gcagcttcga caaaacgttc aacagcagcc ttgtcgtgac
    21181 ggacaaggcc gacccgaacg tgaaggtgcg attcgtcatc gagcgcatgt gcaatgactt
    21241 cggtccggcc accgagacca agtgcatcct ttccgaaaat gccgaacgcg ggggatccca
    21301 gcccctggac aagctcggct cggcggcgct gccgatgtac cggatcacgg tgcgcagcga
    21361 cggcgtccgc aattcccaga cttatgccca ggcggttgtg accacccgcg tgaagtgacg
    21421 cggccgcgtt gccgaggaag tgccatgaaa ctgtcgaaaa gcctgtcccg attccttctg
    21481 atcgtggcga gcgcgagcgc gctcttttcc gctggcctgc aggacggacg cgccgcaagc
    21541 acaccgcttg ccgaccagcc gctcctgacg gcacaggtgc cgtcgaacgt gatcctcgcc
    21601 ctgtcggtgg aatttccgac cgccctcagc gtggcctaca acacgaaatt ttctgtggac
    21661 gggtccgcct acctgggcta tttcgatcca aagaaatgct acaaatacga ttcggccaac
    21721 aattatttcg taccgcaggg gccgagcggt acgaattaca cctgtacgag tgcctggagc
    21781 ggacatttcc tcaactgggc cacgatgcag gccatcgata ccttccgctg ggccatgacc
    21841 gggggcaacc gggtcatcga tctgccggtg aatttcaccg acacccagcc ggtcaagacg
    21901 gtcctgcagc gtgcctacgc gtcctcggac ggcagtgaat gtacgtattt ccccggccgt
    21961 ggcatcagcg cgagcgaccg tgcgaattac acggatacgg cgggggtggg atatacggat
    22021 aagggcctct ggatccgcaa cggcgggcag ggatatcagt ttcaggtggc gccgcagatc
    22081 ggcggcgctg caaattgcag ttcgtggggc ggcggcagtg gttcggttca gtcctattac
    22141 gcccaggtcg aggtctgcaa gaacgccaac ggttcggatg gcaagaccct gctggaatcc
    22201 aattgcacga tgtacgtgga tcctaccggg accaagacgg cctatcgccc gatcgggctg
    22261 atgcagcaat acaagctggc catgaatttc ggagcctttg gctacctcta cgatggcagc
    22321 agcacgcggg atggcggcgt gctccgcgcg ctgtcgcggc ccatcgacct ggagatttcg
    22381 gatacgggag cgttttatcc ggatcccttc gggctggcca atgcgagccg cggcatcatt
    22441 tacagtggta cggccaatta tctcaacaaa ttcggcagta cgtcccttca ctacaaatcg
    22501 ctcgatccgg tcagcgaaat gtatgcggag gtgatgaagt atctgaaggg gatggatgta
    22561 acaccgtcgt atgtatcgac catcaacaat tcgatgcggg acgggttccc ggtgttcggt
    22621 gagtctggcg gtccgacctg ggttgatccg gcgcgggata ccagcgatcc gtcctacccg
    22681 aacagtccgg cgctgtggtg caagaaaagc tacatcatcg ggatcggcga taccaataca
    22741 tggaacgacc agaacctgtc gggacggacg gacgtgtcgg tgccgagcgg tgttgatacc
    22801 aatcttgccg gcggcacggc aaaaagctgg accgataccg tcggaacgac cgagggctat
    22861 gtccccagtg gctacgcaag cctgggtgtg gcgcagccga atcctggcca ctccaacagt
    22921 tttctgatgg cgggtattgc ctattacgcc cacagcagcg acatccgcac ggatctgaag
    22981 gacacgcagt cggttgatac ctactggatg gacgtcatgg agggcgggat ctacaaggac
    23041 aagaaccagt tctggctggc tgccaagtac ggcgggtaca agaagccgct caatacggcg
    23101 atgggtccgt tcaactcgtc caccgatttc tggaaatcac aagggaaggt catccagact
    23161 aagagcgacg ggacggtcgt cacgacgggt ggagccaagt cttatgacct gccggacaac
    23221 tatttcccgg ccaacaaccc ggcgaacatg gtctcttcgc tgcagagcgc ctttgcccag
    23281 atctccgccg ggacgggtgc cgcagcgggg gttgcgctgt ccaatccgca ggtgaatgcc
    23341 gcaagcggcg gttcgatgac gttccagggg acatacgacg caagcacctg gtccggaaac
    23401 gtcacggcat cgaatatcac ctcattcaag gatgatggca cgccggtgac cacgtcggtc
    23461 tgggacgccg cggccagatt gaattcgatg gttgcgagcg ggggatacag ttccaagcga
    23521 aaggtggtga ccctggttcc gaccaaggtc gataacgcga cgcccgcggt tgccgatctg
    23581 gtgggcaagc ctttcctgta cgccaacctg agcacggccc agaagctcaa cctgagcaag
    23641 acgtcgctcg gcggcgatct cggcgatgga gacaaggtac tgaattacct gcgcggtgac
    23701 cgcacctacg agaccaccgc cacgacgccc aatctttacc ggctgcgggg ttcggtgctc
    23761 ggcgacatca tcgattccaa gatcgtctat gtggcggcgc cgaatgcggt gtattccgat
    23821 tcgtataatc ccggctacga gaccttcaag aagaatgctg cgtcccggca gcccgtcgtc
    23881 tatgtcggcg ccaatgacgg gatgctgcat gcctttgacg cgaccagcgc gtccacgggc
    23941 ggtgatgaac tctttgccct ggtgccctac gccctgtatg ccggtcccag caatacgccg
    24001 gatgtggatg gcctgcaggc aattgcacga accagctatt cccaccacta ttacgtggat
    24061 tccacgccgg aggttcgcga cgtggatttc atgcgggcgg gtggcgcgac ccagtccgat
    24121 gcaaccaaga gcgactggcg gaccttgctg gtggccggtc tgggcaaagg cgggaagagc
    24181 tttgtggcga tggacgtcac cagcatctcg tcgggcctga ccgagagtca ggtggcgggc
    24241 aaggtgctct gggagttctt ccatccggac atggggttca gctttggccg gcccctcatc
    24301 accaagaccc gcaagtgggg ctgggtggtg atcctctccg gcgggtataa caacatcggc
    24361 ggcgatacca aggccggtta caagcctggt cagggcgtcg tcttcgtcgt cagccccaag
    24421 gacggcagcc tgctgcaggc gatctacaca ggggctggcg atgcggcaag tccggccggc
    24481 ctggcgcaga tcacgggcta tacccagaac taccaggact acacgacgga ttatgtctat
    24541 ggtggcgacc tcagcggcaa tctctggcgc ttcgatttca ccgacagcag caagagcgtg
    24601 ccgtcgccca cgaagattgc ccagttcaag gatcctgacg gtacgcccca gcccatcacc
    24661 gtggccccga aggccgaata ttcggctgac gatctgaagc gctacgtctt cgtcggaacc
    24721 ggccgcctgc tggcttccag cgatcagaag aatgcccaga agcagacgat gtacgccctt
    24781 cgggatggca cgcgcaccta tgcgttcgga tccaacacca atcaggtggc cttgcccgac
    24841 ggtgtgagtt ttccggtcgg tcgcggcagt atgagtggag tcagcgacct gcttgccggg
    24901 gcgacgaagt ccgagagcac gccgatgggg tggtattacg atctcacggg tgtcacgagc
    24961 ggtgtcaccg agcgggtcgt gctggccctg caggccaatg atggcgtgct gtcctgggtc
    25021 ggctccctgc cgaacagcga tccgtgcagt ccgtccgggg catcgcgcct gtattccgtg
    25081 caatacggta gcggccagag cgtcttctat gaattggtca atggccttcg ttcgccggtt
    25141 gcgtacctga gcatcgatca ggggctggct gacatcacgc tggtgcgtta cggctccagc
    25201 atccgggtcg tcggaacgga taccaagggg ggggccaagg tctatggcaa cacgatcgcc
    25261 gagtcgtccg atccgcgcgt cgtgaactgg cggatcatcc gtgaataggg cgcccgcgtg
    25321 cggcaggact tgaaccggta agggcggatg acgtaagatg cgcgtcctcg ctgctgtagc
    25381 tcagtcggta gagcaactga ttcgtaatca gtaggtcacc agttcgattc cggtcagcag
    25441 caccagaacc ccgtcaacgg ctcccgcaag ggggccgttt tcatttgggc ccgtcccgga
    25501 ccggttcagg gggaaatgtg ataaaacggc gtgtcccgtc gggcccctcg ggtggctgac
    25561 gaccgtctgc tgtgagcgag gccatcgttg tcccattccg taccccctca ggaacacgcc
    25621 tgctatcact gcggactccc catcccttcc gacgttcatc tcggcgtgac ggtggcaggt
    25681 gtcgagcggt ccatgtgctg taccgggtgc caggccgtgg cccagtccat cgtggatggt
    25741 ggtcttgtcg attactatca gcgccgcgac gccatgccgg aacccgtgcg ggaggccatg
    25801 ccggacgaac tgcgcgatct ggggctcttt gatcacccgg atttccagca gggtttcgtc
    25861 aagcccgtcg gtgagcatga acgggaagcg tccctgattc tcgaaggcat cacctgcgcc
    25921 gcgtgcatct ggctcaacga gcagcatgtc gcacgtcagc cgggcgtcat ggcggtcgag
    25981 atcaactacg cgacccggcg tgcacgggtg cgctgggacg aacgtcgcat caagctctcg
    26041 aacatccttg cggcgattca ggccatcggt tatcgggcct atccctacga tgccgcccgc
    26101 tcggagcaga tctcccagaa ggagcgtcgg gcggccctgt ggcggctctt cgtggctggc
    26161 ttcgggatga tgcaggtgat gatgtacgcg gtccccgtat atatggccgg agaaggcgag
    26221 atgaccgccg acatcgaggc catgatgcgt tgggcgagcc tgatcctgac cttgccggtg
    26281 gtgctttact ctgccgcacc gtttttccgc aacgccctgc gcgatatccg cttgcggcgg
    26341 ctcggcatgg acgtgcccgt tgcgctcggg gtgggggctg cctccgtggc cagctgctgg
    26401 gccaccttcg cgggccatgg cgaggtgtat ttcgattcgg tcacgatgtt cgtgttcttc
    26461 ctgctgtgcg ggcggtatct cgagatgttg gcccgtcagc gtgccatacg gggcgtggaa
    26521 gagcttggca aggcgttgcc ggcctttgcc gaacgcctga cggccttccc gtcgctggaa
    26581 ggcgagcgcg ttcccgtctc ccagatcgtg tcgggtgaag tgctgcgggt gcggccgggg
    26641 gagaccatcc cggtggatgg tgtggtcatc gaggggacga gcgaagcgaa cgaggccttg
    26701 ctcacgggtg aaagccgccc cgtacccaaa ggcccgggcg tggatgtcac cgggggcagc
    26761 gtgaacgtgt ccagtccgtt gctcatccgc gtcacccaga ccggcgagca cacccgtctg
    26821 gcggcgattc ggcagttgat ggaacgggca tcggcagaaa agccacaggt ggttcagcag
    26881 gccgacaagg ttgcggcata ttttgtggtg gcgttgctcg tccttgcgtt tgccacggcg
    26941 gtgggctggt ggtggttcga tccagaccgg gccttgtggg tgttcgtgtc cgtgctggtg
    27001 gtgagttgcc cctgtgcgct gtctcttgcc acgccggtgg cgctgacggt ggccaccgat
    27061 gccctcgctc gcgttggcgt gctggtcacc cgtggccatg ccattgaggc actggcgaag
    27121 accgagcaga tggttttcga caagaccgga accctgacct atggcgacat gcgtgtggaa
    27181 gcgacctgtc tgctcggctc ccgcagcacg cgggatgtac agcgactggc cgccgcgctg
    27241 gaacagggat ccgagcacgc catcgcacat tcgctacgga aactggtgac ggacgcaccg
    27301 gatcaactgc ccgttctgtc cgacgtgacg gtcgtgaccg ggcagggtgt tggcgggagg
    27361 ctcgacagtg agcgggttcg gatcggccgc ccggaattcg ttgcggaaat ctgccctggg
    27421 accttacccg ccgaactgac atccgcccaa gcctcgggca agacggtcat tgcacttggg
    27481 ggggagtcag gctggctggc aggcttcttc ctttccgacg tcctccgcga ggatgcccac
    27541 gatgtattcg acgcgctgca cggggacggc gtgaccaccg ccatcttcag tggcgacacg
    27601 cagggcgttg tctccgaact ggggaaggcg ctgggcgtca ccgaggccgt cggagagctt
    27661 acgccggaag gcaagcacgt tcggctcagt gccttgcagg cccacggcaa ggtcgtggcc
    27721 atggtgggcg atggggtcaa tgatgcgccg gtgctggctc aggcccaggt gtccatcgcc
    27781 atgggaggtg gaacggatct ggcgcggaat caggcggaca tcgtcctgct gagcggacgg
    27841 ctcgggtcct tggtcgatgg catgcgcctc tcacgtcgtg cactgcgtat catccatcaa
    27901 aatctgtggt ggtcctttgc gtataacttc accgcggtgc cgctggcgat gtcgggcttt
    27961 gtgacgccgt ggatggctgg tctcggcatg tcggccagtt cacttctggt cgtgctcaat
    28021 gcgttgcggc tgcagcgcaa gcacggaaag gttgcagcct gatggaaagt ctttacctgc
    28081 tcattcccct gtcgatcgtt ttggtcttcg tgatcggtgt ggttttctgg tggtcggttc
    28141 gcagcggtca gttcgacgat ctggaaggac cgggtttccg tgtcgtgacc gacgacgaca
    28201 cgccgcggac ggagacggag agccccgtgg agcgcaaggg tacgaccgag cggtcatgac
    28261 cagggcgatg gattcaccct gcaagctgaa tggtttttga cgcaggtcaa gaccccgtac
    28321 agaagagatg ggcatacttc acgccaagtt gatgacgcga acacgagcaa caaaatgcgt
    28381 tgtcagggcc cgtcggcccg atgacttaag tctctctgtt ggggttgggg gtgcgcgatg
    28441 cgcacccttt tttttcgtcc ccacggtctt gaagccgact tgatgcatct cgcgcctgcg
    28501 cgtcagcgtt ccgaaattcg tggcttgatg ccggaatccc tgaagaagat atgcacggtt
    28561 tgaatgcgta ctctcccgag cgtgagccct gcggtaaagc gcggtgcgcc agtggcttgc
    28621 aaagggggcg ggcagttctg atggtgaggg gaccgtccgc ccgcacgacc atctcttcgc
    28681 ctcggatcaa ggtgcacggc cggctctttg cggtgctccg ggggcaccgc gggaggcttt
    28741 ggaggggtgt cgctcccgag tttcctgagg gttggcagcg ccttgttttt tcggccattt
    28801 cggtgcatgc ctcacgcgtt ttgcaccccc ttggtgcggt tgcgaaaaca ttcgacggcg
    28861 cccgcgggcc ttgctattgt gtgaattcgc ggggcattcc gacccgcctt caggcacatg
    28921 gagcgcgtca attgggtaac aggctttacg tgggcaatct ggcctacagc gtggacaaaa
    28981 cccgtcttga agagggtttc cgggagttcg gcacggtcgt gtccgcacag gtgatcatgg
    29041 atcgcgaaac cggtcgatcc aagggtttcg gtttcgtcga gatgggctcg gatggcgagg
    29101 cccaggcggc gatctcggcg atgaacggca agagtttcga tggtcgcaat ctgaccgtga
    29161 atgaagcgcg cccgcaggag ccccgctcgg gcggtttcgg tggtggcatg cggcggagcg
    29221 gtccgggcgg tgggcgccgg ggcccgacgt cgggtggtgg cgaccgttac tgatcgcgcg
    29281 cagccggcgt gaagtgccgg ctgcaagagt tcctcttcgt gaggtaggct cgccgaccga
    29341 tccacccggg tgctgcgctc aggcctgccc gggagcatgt ccgagccgcc agacacatga
    29401 cgtctgcgcg aagcgccggt tagcccggct caggtgttct cgaattcttc caccatcaac
    29461 tgtacgctgg ccacgccgtt gtattcgttg acggccaggc ggaaggcggc gtggatggct
    29521 gcgcccgccg gatctgcgtg attgaagcgg atggcgtcga tgcgcagggc acctttgcgc
    29581 aattgcagct tgaggtgctt gtccttcagc aggcgctggt tttccacccg gaagcagtcg
    29641 tcgaagagcg gcgccggaaa gccctgcccc cagatctggt tttccagcag gcgcgctgtt
    29701 tccagactgt agaacccctc ttccagtgat ccgtcggttt ccacgctgcg ggtcaggtcg
    29761 gccggatcaa tcatctgacg gacgacgcgt tcgaacgcat cacggaacgg gcccagatcc
    29821 ggctcgcgga tcgtgagacc tgcagccatt gcatggcccc cgaacttgag gattagatcc
    29881 ggcgcctgct tggtgacgag atcgagggcg tcgcgcaggt gcaggccggg aatggaccgg
    29941 ccggagcctt tgagttcacc ctcatgactg cgggcgaagg cgatggtcgg tcggtggaag
    30001 cgttccttga tccgcccggc gagaatgccg atgacgccct gatgccagtc gggctcaaac
    30061 aggacgaggc tgctgcaatc gcccggcgag aggccatcca gtccagccag ggcaccttcc
    30121 tgcatgtcgg cttcgatggc acggcgttcc cggttgaggg cgtccagttc ctgggtgatc
    30181 tgcagggcgc gactcgcatc gtcggtgatg aggcattcga tgccgaggga catgtccgcc
    30241 agacggcccg cggcattgag tcgaggcccc agcgcaaagc ccaggtcgaa ggtggtggcg
    30301 cggccggcgt cccgtcccgc ggcggcgaac agcgcgcgca ggcccggttg catacgcccg
    30361 gcgcgcatgc gttgcaggcc ctgggcgacg aggatgcggt tgttgtggtc cagcctgacg
    30421 acgtcggcaa cggttcccaa tgcgacaagg tcgagcagat cggcgagctt gggctctgcc
    30481 tggccatcaa aggcgccacg actgcgcaat tcggcgcgca gggcgagcat cgtgtagaac
    30541 atgacgccca caccggccag tgccttgctt ggaaactcgc agcccggctg gttgggattg
    30601 acgatcacgt ccgcctcggg cagttcgtcg cccggcagat ggtggtcggt gatgagggtg
    30661 gcgatgccgc attcgcgtgc ggccgcgacg ccgtcgacac tggcaatgcc attgtcgacg
    30721 gtgatgatca gttccggctg cagggcggcc gccagttcca cgatggctgg cgtgaggccg
    30781 tagccatact cgaaacggtt ggggacgatg tagtgcacgt cggccccgaa ggcccgcaac
    30841 gcgcgcatgc cgacggcaca ggctgtggcg ccgtcacagt cgtaatccgc cacgatgacc
    30901 atccgcgcgt tggcttcgat ggcgtcggcg aggagctgcg cggcggcacc gacgcctttc
    30961 agctggtttg gcgggagcag gtgctggagg ccgtaatcga gctcatgacg ggtcgcgatg
    31021 ccacgggcgg cgtagatgcg cgccagtaag ggatgtacgc cggtatcgag gagggcggtg
    31081 acggcctggc cgggggccga gcggctgagg atgcgggtca tggcgcggtg gtggaggcga
    31141 cgtccgaggc ggggttcggc gtttggcgca ggagggtgtc gagggcgagg ggcttgcgcc
    31201 agaatttcca gaggctcgcg gcggtgacgt ccagttcaag gcaggcccgg tcgccagggg
    31261 cggtgatgcg tagccggctc aggtgtctgg acctgagcgc ggccagcagg ggcgcgaacc
    31321 agtcccgttc gatcgtgccg agcgtcgcgc gccaggcgtc cgggtcccgg tgcagcagcg
    31381 gacgccgaag ggcggtgagc agcgtgaagc cgtcggtgcc cggcgcggcg tacggtccgg
    31441 acgaccgggg cgcgccgccg ctcgcccgga tcagaccaca ggtgtagggg tcatcggcct
    31501 gaaggctggg cgctggggca atcggcgtaa gcggctgccc ggcaccccac agccatgcgc
    31561 tgttgatggc gcgctgtccg gccgcttcgc gggctgcgtt gacggggtgg ttgaacagcc
    31621 acacctggag ttcgttgctc aggcgcgccc agaacggaat gtcgtcacct tccggcagaa
    31681 agagctggat gggacgtccc gtcacgtcgg ccaggggatg gaagaatgga cgcggctcgc
    31741 gctcggcgcg cagataccag cggtcgggcg ccggagcctc gaagcgaccg atgtcggcaa
    31801 aggtctcgtt gaggccggcg atcaacgtgt cggcctctgc ttgggtgatg tccagaccgc
    31861 tggcgtcggc gaggagcagc ttgtcccggg cgaaatgcag atgaaccggg tctatgcaca
    31921 accagtgggc gctgcgttcc gggcttgtgg tggcttcgcc gaggcgtcgc aaccaggcat
    31981 agggaatacg cttgccggcg agctggaagc gatcggcgag ccacgcttca ggtgtgcacg
    32041 ctgcacccca atgcacgcgg gcgtggccga tgagggctgc cagggccggg agctcaaggt
    32101 cggcggccgg tccggccagg ccggaagcgg gccacacgag gccgggaagg acgaggtcgt
    32161 actgcatcgg gtcgagaatg ggtgggctac agcggcgcgt tgggcagggc gcaggcggtc
    32221 gggcgcgagt ctagcatggg ctctgcgggc tcagggttgc cgcggctcga tgagtgcgga
    32281 agcataggcg gccgagcagc ggagggcctg ttcgggcccg aggttgcggg tttcggccag
    32341 caggcagcgg ggatcattgt caccttcgag gacttcggcg ggcagatcgc gcaggctgcg
    32401 ggcatcgatg ggcgtggcca gaatggcgcc cgcggcctgg aagccggccc gcacgaatcg
    32461 ccgcggcgcg tccgtgacga gcaccagcag gggcccatcc ggcgtgccgg cgagggaagg
    32521 gctgatctgc acgccgggtg ccagatgcag ccggctgtcc gaggcatccg gcagcagcag
    32581 ctcgaacccg cgcccatccg atggcgcatg caggacgtag aggaaacctg cccgcggtga
    32641 gcggatgagg agtctgtcat cctgccggcg tacgtcgagc ggtcgatctg cagcccgctg
    32701 ggcatgcagg atgcggagga gcgccgcggg cgtaaagccg ggcgctgcag caggggtcag
    32761 cgtcggaagg ggcacaaggt cggcattgcc aacccgttcg aggcggcggg cgcccgggcg
    32821 cgcgggaccg agttgtgcct ccgcgcagcg acgcaggtcg tcgatcctgg caagtccact
    32881 cgtactcgtc acgggggcat cgcccgacag gcaggccagc agcgccgcgc tggccgcacc
    32941 gaagtccgga ctgtcggagg caagcagggc gagattgggg ggggagttgg gcaaggcttc
    33001 ttgccagtgc tgaaggaggc cgtgactgcg ggacggcaca tccgttcgtc tgtccggggc
    33061 catggtctgc ggggcgggcg gggctgcgtc gacgacgatc agcacctgcc cggccttgcg
    33121 ggcgagcgtg tcgaggtaac gacccagttc ggcgacctgg acgcgaccgt caggggtggc
    33181 cagggccgct gcggacgggg cccgggctgc gcgcggtggt tcggtacggg cgaggatgtg
    33241 tacgaacacc cggtcgccgg ggccggcgtg tgcgtccaga tcggcgaggg cacggcggat
    33301 gcggtcgggg gtgaggctga cggggtcggg ttgctggatg ttggcatcgg ggacgcccag
    33361 tgcccgggcg agctggcgtg cggtcagatc ggtcgcatgc gatccgggcg ggacggggtc
    33421 gtgatcggtc aggtccagga gcagggcgtg gcgactggcg tggggctgga ggcggggacg
    33481 ggcaccgctt gccgcaaacg cggtcggcgc ggcaggggct gacggactcg ccgggggttc
    33541 gtcgaggcgc aggctggccg cggggaccca accccggcgg ttgcccgcag catcgaccag
    33601 gtaccagtcg cgccgggcgt cggcgatgtc cacgctgctc aggcggtgca gcggcatgac
    33661 gatggcggcg tcgctgcggg gctgggcgcg cagggcgtcg tcgtggatga cggtggcacc
    33721 gacagcgtgg acgatacccg tggcgagcag cagggccgtg gcgaacgccc tgtgaaaggc
    33781 ggggaacctt ggggggtgaa aacgggtcat tggtgcgtcg cgccgggcgg cgtccggtgg
    33841 ctgaaggtgc cattctgacg gtccggggtg gccggtgcca gccccggacg gtgcgggcgc
    33901 tgtcccgttt cccgaccaag tccggcacaa tgcgtggctg cgccggcgcg aggcgttttt
    33961 ttctggttcg ttcatgtcct acgaatttct ggtcggtctg cgttacaccc gttcgaagcg
    34021 gcgggcccgc aagggaaacc gcttcatctc attcatctcc ctggtgtcga tggcgggcat
    34081 tgcgctgggc gtggcagcgc tgatcgtggt gctgtcggtg atgaacggct tccagcggga
    34141 actgcgcacg cgcatcctcg gtgttgcgtc ccacgtgcag gtcaccggct tcgacggcga
    34201 gctggccaac tggcagcgca tcgcggacga ggcatcgaag catccgcggg tgctggccgc
    34261 ggcgcccttc gtgcaggcgc aggggatgtt gtcctttgat cagacggtgc gtggcacgct
    34321 ggtgcggggc atcctgccgg atgcggagga caaggtggcg gatttcgcgc agcacatgcg
    34381 cgccggtcgg ctcgacgaac tccagccggg gggcttcggt atcgtgttgg gcgccgatct
    34441 ggcgcgcgcg ctgcgcgtga ccgtgggtga caaggtgacg ctcatcgcac ctcagggcct
    34501 ggtgacgccg gcggcaatcc tgccgcgcct gaagcagttc cgggtgacgg gcatcttcga
    34561 ggtcggcatg tatgagtacg actccagcct ggccttggtc catctgcagg atgcccaggc
    34621 cttgtaccgg atggaagacc gggtgtccgg cgtgcgactg aagctcgacg atctgtttaa
    34681 ggcgccccag gtggcccgcg agctgatggg ctacgttcag gcggatacgc tgatttcgga
    34741 ctggacccgc gcccatgcca atttcttccg ggcggtgcag atcgagaaga acgtgatgtt
    34801 catcatcctg ctgctgatcg tggcggtggc ggccttcaac atcgtgtcca cgctggtgat
    34861 ggcggttcag gacaaacggg cggacatcgc catcctgcgc acgctggggg ccagtccagg
    34921 ttcgatcatg ggcatctttg tgatccaggg cgcgctgatc ggggtggtgg ggctcgccat
    34981 cggcgtgatc ggcggtgtgg cgctggccct caacatcgac gtggtggtgc cggccatcga
    35041 gcggacgttc ggcatccagt ttctggccaa ggacgtgtat tacatctccg acctgccgtc
    35101 gcaactgctg tggagcgatg tcggcgtgat tgcgaccgtg tccttcgtgc tgacccttgt
    35161 tgcgaccttg tatcccagct ggcgggcatc gcggctcaac ccggcggagg ccctgcgcta
    35221 tgaatgaacg gacgtccgat ggggtgattg cctgccgtgg gctcgccaag actttccgtg
    35281 aaggcgatga cgcggtgagc gtgctgacgg acgtcaccct ctccgtgcaa cggggcgagc
    35341 gggtggctgt ggtcggggcc tcgggctccg gcaagagcac cctgttgcat ctgctgggcg
    35401 ggctggatgt gccgtccgcg gggtcagtga gtctgctggg gcgggatttc tcgacgatct
    35461 ccgatgcgga gcgggggcgc ctgcgcaacc agtcactggg tttcgtttat cagtttcatc
    35521 atttgctggc agaattttca gctctggaaa atgtggccat gccgctgctg gtacggcggc
    35581 tgccccgtga ggaggcgctg gcgcgggcgg ccgaggcgct gaaggacgtc ggactggaac
    35641 accggatgga tcacaccccc ggcgagctgt ctggcgggga acggcagcgc gcggccattg
    35701 cgcgggcgct ggtgacccgg ccggcctgtg tgctggccga cgaacccacc ggaaatctgg
    35761 accgccagac ggcggaacgg gtgttcggat tgatgctggc gctggcggag cagcatggca
    35821 cgagtttcgt catcgtgacc catgacacgg cgctggctgc ccggacgcac cgcgtcctgc
    35881 acctgcggga cggtcgcctc gcaggggaaa cctgactcgg cagcggggtc ttccgcgtac
    35941 ggaaacatga catcgtggca gaaggcgctc tcaagtttgt gggcgcggca ccgatagagt
    36001 gcataataat tctgttgaat taaatgtttc cgacgattca aaagggagat gaacatgcac
    36061 ctgctctttg cgccggccgt cgcattgctc aatcgaatgc gataccgggc caagtttgcg
    36121 ttgctgggcg ctctggcctt tgcggtggtg ctcttcctgt tggggcagtt ggcgttttcg
    36181 ctgcgggaaa gcatgcactt caccgaagcc gaacggtccg cgctgggcgt ggtgccccgc
    36241 ctgctgacgg tcatccagct gacgcagcag caccgcggcc tgtctgccgg agtcctcaat
    36301 ggcaatgagg gcatgcgcat ccgcctgacc gccaaaacgg gggaactggg tgaggccatg
    36361 aaaaaggtcg atgccattgc acttggcgcg gacgcctcga ttccgatggc acggtggggg
    36421 gaagtgaaat ccgcgtggga ggtcctgcga gccaacggct tgaatcttcc gccgaaggaa
    36481 aatctcactg cccatagtcg cctggttgcg atggaggtgc tggcgctgca cgatctgggc
    36541 gacgacggca atctgaccct cgatccggtg gcggatacct attacctgat cgacaacctg
    36601 ctcaagcgtg ttcccgaagt gacggaacgt ctcggccgcc tgcgtgcgct gggcacagga
    36661 gtgctggcgg ccaagagcct cgatgagcag aagcggtacg acatcagcgt gcagttgggg
    36721 gagctggaca tggcggtcga agccctgaac gaaaacttcg accgggccat ccgtttcaat
    36781 ccggcgctca agggatcgct cggtgccttg cgtgatggtt tcaatgcggg aaccggcaag
    36841 gtgctggacc agttgcagaa tcatgtgctc aagggggatt tcgagatggc gccgcaggcc
    36901 tatttcgatc tggtcactca ggcgatcgac atcgcgtatg ccaagacctt cggagagttg
    36961 atcccggcca ccgacgagct gctggccgcg cggattgccg gtcatcagcg gagtttcgcc
    37021 atcgatctcg tggttgcggt cctggtgatc ctggtgctgg cctacctcag cggcgcgacg
    37081 tacgtctcgg tcaatgatgc ggtgacccgc ctcagcaatg gcgcggaatc gctggcgaat
    37141 ggcgatcttt caacgcgcat cggtctggaa agccgggacg aacttggcga cgtcgccgag
    37201 cgtttcaacg cgatggcggc tgcgttctcg gaagtcatcc gcaaggttca gcaaggagcc
    37261 gacgcggtgg ccgggtcttc cgtctcgctg gcgggatcgg ccgcccgtgt gtcggatggg
    37321 tcggaacagc aaagcgcggc ggcgtcgagc atggccgccg cggtggaaga gatgacggtg
    37381 gggatcgagg agattgccgt caacgccgca tccgcggaag gcgtgtcgtc ggagtcgggg
    37441 aaattgtccc aggaaggggg ggcggtggtg gcgcggacgg tttcggagat ggagcggatc
    37501 gccgacgcgg tgcaggaatc tgcccgcgtg atccaggaac tcggcgacca gtccgcgcag
    37561 atctcgacca tcgtcaattc catcaaggaa attgccgacc agaccaatct gctggccctc
    37621 aatgcggcca tcgaggccgc acgggcgggc gagacgggac ggggtttcgc ggtggtggcc
    37681 gatgaggtac gcaagctggc ggaacgtacg gccaaggcaa cggaggagat cacgaccatg
    37741 gttgcgtcca tccaggccgg cacgggcaag gccgtggaca ccatgcagga tggcgttcgc
    37801 cgtgtacagg aaggcgtcca gctggcgggc caggcgggta tttcgatgga tcagatccgc
    37861 agcggtgccg ggcgggtggt gactgccgtc accgatattt ctgccgcctt gcgggagcag
    37921 ggggtggcca gcaccgaaat tgcccgcaat gttgaacgca tcgcccagat ggccgagcag
    37981 aacagtgccg cggtccggga tacggcaggc accgcccggg aactggagcg cctcgcgcaa
    38041 caattgcggg gcgacgttca acgtttccgg atctgagcgt ctttcaacag ggacgacggg
    38101 gcgcctgagg cgcccttctt acttgagcga cggaccgtct gccgacggac ggcggatctc
    38161 aatgcgggag cagggcggtt tccagttcct ggcggccgag gggtgaggcc atcgcgatga
    38221 catgctggcc aggttgcagg cggaagtcct tgggcggatt gaagacgaag gtctcgccct
    38281 cgcgcacggc cagcagcaca tagttcgggc tgcgtagctg cagggtaccc agatcgcgtg
    38341 gttcgaagcg cggcggcacc tgcacttcct cgagacgcag tttcttctcg gagcgcagca
    38401 tctcgtccag gaaggacacc acatgggggc ggaccatgct ggacgcgatc cgcatgccgc
    38461 ccgtgaagtc cggtgagatc acgctgtcgg cgccggcctt gcgaagtttt tcggcattgc
    38521 gcacttcatg acagcgggcc accacgcgga tcgacgggtt gagctgcttg gccgtgaaga
    38581 cgatcatcag attgcggctg tcgtcgcccg tcaccgcaaa cacgccctgg gcgtcctgga
    38641 tgccggctga catcagcagg tcgtcgtcgc ttgcgtcacc gaccaggtaa accaggcccg
    38701 ggctgcgttc ccgttgcgat tccatgccgg cttcatcgca gtctatggcg acgaagtggc
    38761 ggttcgtggc ggtcatttcg ttgcccacgt tacggccgac gcggccgtag ccacagatga
    38821 tgaagtggcc gtggagcttt cggatctgct tttccatacg tcgtctccgc aggctgtaat
    38881 cgaggtcact ctcgaggaag aagaccgtca ggctggtgaa cagaaaggtg acggcgccaa
    38941 agccggcaag ggcaatcagg cccgcgaaga ggcgctcggc gatgccgtcg atgggcacga
    39001 cctcaccgta gccgactgtg gtgaccgtaa tcagggtcat gtacagggcg tcggacagat
    39061 tggcctgctc gccgcccgct gcgtaaaagc ccaccgtgcc aagcacgatc aggcccagca
    39121 gcacccccgc cgcgaaatag atgcgcagca ggatgcgcgg gagctggctg cgccgcgact
    39181 gtcgcatcgg gatgcgccgc ttgggcgccg gctgttgcca ggcagtgacg aactcgtcga
    39241 gcttcatgcg agccctttcc ggcggcgcca ggcgcgcacc agatggatcc gccatgccag
    39301 ccgggtcagg acatagccgg aacaggcgag cacagcggcc agcgcgacga gccccagagc
    39361 caggggccgt cccaggtctg cggcccagtg ggcgagttgt ccgagccagt gaaggagact
    39421 gccgtcaaga tcgggtggcg cgatgaacgt ccggccgtcc ggaccgagga tccactggcc
    39481 gagggtgaag gcggcgagat acaggggcac gatggtgaat gggttggtgt agagggtcgt
    39541 gaagagtgcc aacggcagat tgaccctgaa catgacacta cagagcgccg cgcccagcat
    39601 ctggaagggc ccggggatca ggccgcagaa caggccgacg gccacggcgc ctgcggcgga
    39661 atggcggttg agatgccaca ggcggggatg aagcagggga gcgccccacc acgccagcca
    39721 gcgatggtcg gcgagggatt ccggcgtagg cagatggcgc ttgagcagtc gtcgcatggt
    39781 gcgtcacggt gcgaccgttc tggtcggacc gggcgagcgg gaaaatctcc cccgccccga
    39841 ttctactcta cgcccggctt ggtgtccggg gcggagcagg gtagattcgc ggtcatgctc
    39901 atgtcaattt ccggcttcct cgtgggtgtc gtgtcgtgcc agtttggggc agcgcttccg
    39961 gcaccgtcct gccagtttgc agccgccgta ctcgcggctg gctgtgggct ggccggtgcc
    40021 cttttgtacc gctcgagccg ggaacgatgg ccagggctgg ccctggtgct gtcgatgctc
    40081 gccggcctgg cggccggtta cgcctacgcg ggctggcggg cagagttgcg ccttgcccag
    40141 tccctgcctg gcgactggga ggggcgcgac gtggcggtga cgggcatcgt ggcgaccttg
    40201 ccgcagaatt tcgggcgggg tgtacgattt gtgttcgagg tggaggcacc tgcagggggg
    40261 gctgtgccgc gccgaatttc cctggtctgg tatcgcggcg ctgacgacga cgaggccggg
    40321 ctgctgggcg aggtgcatgc aggcgagcgc tggcacctgc tcgtgcggct caagcgaccc
    40381 cacggaaacg tcaatcgtca tggttttgat tacgaggcgt ggttgctgga gcaggatctt
    40441 cgcgcgaccg ggtacgtgcg ggcagacgcg tcgaaccggc ggctggacgc cttcgtgctg
    40501 tcgcccggca cgctggtcga gcggctgcgg gaggtcgtgc ggagccgctt ccaggccgcc
    40561 ctgcctgacg ggcgttacgc cggcgtgctg attgccctgg ccatcgggga ccagcgggcg
    40621 atcccccagg agcaatggtc gctgtttgca cggaccggca ttgcgcatct ggtttccatc
    40681 agcggcctgc acgtgaccat ggtggcggcc ttatgtgctg cggtgatcgg agcgatctgg
    40741 cggcgcgtgc cggccctggc gctccgcgtg cccgcccagc aggccgcggt ggcgaccggc
    40801 gccttagcgg cggggggcta tgctctgctg gccgggtggg gcgtgccagc gcaacggacg
    40861 tttgcgatgc tggcgtgggt ggcgctggcg ctgctgctgc ggcgggaggc agcgccgttc
    40921 cgggtgctgg gccttgcgtt gctgggcgtc ctgctgctcg atccctgggc ggtgctggcg
    40981 ccggggttct ggctgtcgtt cggggcagtg gccttgctgt ttctggcggg cagcggacgg
    41041 cttgccgccg gcgggcgcat ccgggaggcc ttgcgcgccc aatgggccgt gacggtgggt
    41101 atggtgccgg cacttctggt gctgttccag cagtgctcac tggtgtcgcc gctggccaat
    41161 ggcttggcaa tccccgtggt gagtttcgtg gtgacacccc tggcgttgct gttctcggtc
    41221 ttccccctgc agatcctggc ggacgtcgcg catgcggtgc tggggggcgt gatgctgcca
    41281 gtggaatggc ttggtggcct gccctacgcc cagtggcagc aggctgcgcc gcctttcgtg
    41341 ctggcgctca tggccacgct gggctgtctg tgggcgcttc tgcccgccgg cactccggcc
    41401 cgctggctgg gggcgtcgat gttgctcccg ctgctcgcct ggcaccccgc gcggccggcg
    41461 ccgggtgccc tgaagatgac cgtgctcgat gtggggcagg ggctggccgt gcacctgcag
    41521 acggcggcgc acgatctgct ctacgacgcc gggccggcgt tttccgccga cgccaacagt
    41581 ggtgagcgca tcgtgctgcc ctatctgcgg gcgcagggtg ttcgtcgtct cgacagcgtg
    41641 gtggtgacgc accaggacaa cgatcatgcc ggaggcgccg aggcggtgct gggcggcatt
    41701 cccgtggcgg aagtacgctc ttccctgccg acggcccatc cggcacgcct gatggccgcg
    41761 gaccggcatc ttccctgtcg tgccggtgac gcgtgggaat gggacggcgt gcgtttccgt
    41821 gtgcttcatc cgggggcgga ggccgatgcg gatcgtccgc gcaagacgaa caacctgtcc
    41881 tgcgtgatca gggtggacgc tgcggggcac agcgtattgc tgacctcgga tatcgaagct
    41941 tcggacgaag aggccttgct ggcgcgggat ccaggggcgc tggccagtga tctggtgctc
    42001 gccccccatc atggcagccg gacgtcctcc acgccggcct tcgtggcagc gacgcatccg
    42061 gcctgggtac tgtttcccgt cgggtaccgg aaccgtttcc accatcccaa tccggaggtg
    42121 tggagtcgct ggagcggcgc aggcgcgcgg atggcgcgta ccgacgcgga aggcgccctg
    42181 gaggtcaggg ttgatgccga aggggtgcag gtcgagggcg cgcgggcggt ggcgccgcga
    42241 tactggtacg ggcgctgacg cgatcggcat tccacgggct gcagatgccg gactgcatgg
    42301 tgccaaagct tcacggtgcc gatgctatcc ttccggcgtt accactctcg cccttgtcgg
    42361 ccgggtctcc atgagcttga ttgatgccct gcgcaggctg ttcacgcctg cccccgtcgt
    42421 tgtgccaccg gccgcggagg tgtccgccgg aggcttcgag cagcctcgat tccaccgcgt
    42481 ccagtgtgcc ggcccccatg gcctgcatcg aatcgcctat acagagtggg gtgacccggc
    42541 caatccgcgg gtgttgatgt gcgtgcatgg actcacccgc aacggacggg atttcgacga
    42601 cctggcccgg tcactggccc atcattaccg ggtcgtctgt ccagatgtgg tgggccgtgg
    42661 gcagagcgaa tggctggcgg tgaaggacga ttatcagctg cccacctacg tggccgacat
    42721 gatcaccctg atcgcccgcc tgaacgtgga gaccgtggac tgggtcggga cgtccatggg
    42781 cgggctgatc ggaatgctcg ttgccagtct gcccgaacag ccaatccgtc gcctggtgct
    42841 caacgacgtg gggccggtca tcacggtgag ctccctgcgt cgcatcggcg aatacgtggg
    42901 caaggcaccg gtgtttccca gctacgaggc cggcgaggcc ttcatccggg cggtgagcga
    42961 gccgttcggg cgcctcagtg acgcccagtg gcgtcagctc gcggtccatt ccctgaagca
    43021 gaccgaggcg ggctgggaga tgcgctacga cccgggcatc ggcgaaccct ttcgcaagac
    43081 tccggtgctg atggacgtgg aactgtggtc gatctacgac gccatcgcct gtccgacgct
    43141 ggtggtacgc ggggccgaat cggacctgct gctgcgggag acggccgtgg agatgggccg
    43201 tcgcggcccg aaggcaagcc tcttcgaggt ccctcacgtg ggccatgcac ccaccctcat
    43261 ggagccggcc cagatcgccc cgattcgcga cttcctgctc gggagccgct gatgctggcg
    43321 acgctggtga gtgccttcag cgtggtggat tgggccgcgc tggcgtggtt cttcgcctgc
    43381 tggatgggct acggctgggt ggtggagcat ggccccaagg gcggcatggg cggtttgctg
    43441 ggcgccagcc atcgcttccg tctcgaatgg ggccggcaga tgctgcggcg cgaggtgcgg
    43501 gtggcggatt ccgcactggt gggcaacctg atgcagaacg tgtccttcta cgccaacacc
    43561 accatctacg tgatcgccgg cctgctggcg ctgctgggga cggtggacaa gctgatcgat
    43621 gcggcggccg agctgccctt tgcccggcac gtgtcacggg aggtgtggga ggtgaaggtg
    43681 ctcctgctgc tgatggtctt cgtggtggcc tacttcaagt tcacctggtc cctgcgccag
    43741 ttcaacatgt tgtcgatcct gatcggtgcc gcgccgccgc cgggggaggg cagcgaggca
    43801 gacgcccagc gctttgccaa ggtgaatgcg ctggcgggcg atgagttcaa ccggggcatc
    43861 cgcgcctatt acttcggcct cgcggcggtg gcgtggttcg tgcaaccctg gctgttcatg
    43921 gcggtgacca ccttcatcgt gctggtgctg taccggcgcg actttgcgtc ccacacgctg
    43981 gcggcgatgc gggagtgatc actccttggg cttgagcttg accgctgcgg agttgatgca
    44041 gtaacgcagg ccggtcggcg cggggccgtc gtcgaagacg tggcccaggt gagcaccgca
    44101 ctgcctgcag aggacctcgg tgcgcaccat gaagtggctg cggtccacgg cttcctccac
    44161 gttgcccggc gccgccgcac tgtggaagct gggccagccg cagccggagt cgaatttgtg
    44221 ttccgaggca aacagctcgg cgccgcagca cacgcaacgg tactcgccgg cgtcgtgaca
    44281 gtcccaatag cgcccggtga aggcccgttc tgtgcctttc tggcgggtca cgtggtattc
    44341 ctcgggactc agctgggcgc gccattcggc gtcggtcttc tcgatcttgc gggacatggg
    44401 tgcctccggt cggggtgtcc ggatggacgt cgggcggggc cgaaaggttc ccgcccggat
    44461 ggggctcagt gcaggtgctt gggcatctgc tcggcctgct cttcagggag ctcggcgtgc
    44521 accgcctcgc cttccggcga gggatacatg ggcgcgccgc agtcgtcgca gtattccatg
    44581 ggaaactcgt tgtcgagcac ctggatgtcg gtgatgccgc aggcacgcag cgtcgcttcg
    44641 atttcgccga gggtctcgct ggtctcccct tcggcaccga ggagcggcca gaccacgccg
    44701 tggaccacgt cgttgctgtt gcgcagggtg aagccgatgc ggaactcctc cacctgacgc
    44761 tcgaagaagg gcgcgacgac ggcccgcagg cgcgtggccg gggcatccag cgtggtgctc
    44821 aggaaggcca cggacgcctg gatcgagtag gggcgagagg ccttgtcggc ttcgcggctg
    44881 gcggcgaagt aggcattggg caacaccacc tcggtggcac agccggtgag cagcggggcg
    44941 atgcaggcgg cgccctgggc gcgccactgt tcgaaggcct gatcgcgggt gcagtcctct
    45001 tcctgccagc ggaagatggg cgcgcccttg ggcaccacca cggcgccgag gatgtagcgg
    45061 gtgtcggaca ggaactgggc ggtttccggc aggttgtcgg tcttgatgcg caggtcggcg
    45121 tcggtggcgg ccgagcgggc cagatgggag gcgaaatccg ccgtggcgca gtaaccctgg
    45181 ggcagttggt ccggactgaa caggtagtcg gcgagggcga gcttgacatc cttggccagc
    45241 acgtgggcct tgagctggac acgcaggttg gcgagggccg cggccggaat ggtggcggac
    45301 ggaatgcggt aacgcgacca cgccatgacc ggcgcggcga tgaggaggac gtcgtgggga
    45361 tcgtcagagg tgagggcgcc ttcggcgcgg gattcgatca tgtccgccag ctcgtcgtag
    45421 gcggcggcgt tggtgctgct gaggtggtcg agggcggtgt tgagggtgtc ctcgtcctcg
    45481 gcgcggagca ggccgtccac ggccgcggcg aggcgctggt cccagaagtg atcttcggcg
    45541 cggctgctgg attcggccag gccgctggcg agccacagga gctgttcggc gtcccgcggc
    45601 aggccgccgc ggcgtccgag acggttacgt ttcatcgggg ctctccttgt tgtcggtacg
    45661 atgcctggaa ggctgttttg gaaggggcgg cgggcgtgcc cgccggggcc gtcagaacga
    45721 cgaggggttg aggcggactt cctggaggat gatcgtggag atctcctcga tggacttggc
    45781 ggtggagtcc agccagcgga tgccttcgcg tttcatcatg cgttgggccg cttcgatctc
    45841 gcccaggcag ttttcgatgg aggcgtagcg gctgttgggc cggcgctcct gacggatctg
    45901 ggagaggcgg tcgggtccga tggtcagccc gaacagcttg tgccgatggc gggccagctc
    45961 gccgggcagc ttgttgcgct cgaagtcctc ggggatgagc gggtagttgg ccgccttgac
    46021 gccgaactgc atggccagat acaggctggt gggggtcttg ccggagcggg acacgcccac
    46081 gaggatgaca tcggcttcct gaaggtcggc gtgggagacg ccgtcatcgt gggcgagggt
    46141 gaagttgatg gcctcgatgc gacgggcgta atcctggctt tcggcgatgc cgtggaagcg
    46201 tccgaccgta tgggtggagc gctgacccag ctcgctttcc agcggaccga tgaacttctc
    46261 gaagagatcg aggaaaaggg cgtcggattc ccgcagggcg gcaacggcgg ccgggtctac
    46321 gagggtattg aagacgatgg ggcgcaagcc ggtttcggat gccgcctcgc gaatgagcag
    46381 ggcgcagtcg cgggcctttt cgagggagtc gatgaagggc agccgacggg cacggaagcg
    46441 cgtgtccgga aactgggcga ggagactgtg gccgagggtt tcggcggtga taccggtacc
    46501 gtcggagatg aagaatacgg ggcgggtcgg gctcatgggc gatggtgacg aggaatgcgc
    46561 cgggggcgga ccgtaatgtc cggccggaca tcttatccca ttccgcaggg agactgcgcc
    46621 gacccgtgat ccagttgggg ctccacccgc ggcgttaggg aaacccctgc atgcggcgga
    46681 gcagcaaggc gtttacaata cacgcaaaat tctaacgatc ggcgaccgct ttccctttcc
    46741 atttctccca gaaagatctt ctcatggtcc gtaaagtcat cccttttcaa gaacttcgca
    46801 tgtccgatgt ggagcaggtg ggcggcaaga acgcctccct cggcgagatg atcagccagt
    46861 tgcccgccag tgtgcgcgtg cccggcggct tcgcgaccac tgcggcggcc tatcgcgagt
    46921 tcctgtccca ccagggtctg gcagatcgca tcagcgcggc gctggcgacg ctggacgtgg
    46981 atgacgtggt ggcgctggcc aaggccggtg cgcagatccg tcaatggatc gtcgataccc
    47041 cgttcccggc cgcgctggaa gccgagatca aggccgccta cgagcagctg actaccgaag
    47101 gtgacggcag cttcgccgtg cgttcgtccg ccactgccga agatctgccc gatgcgtcct
    47161 tcgcgggcca gcaggaatcc ttcctgaaca tccacggcta cgagaacatc ctgcacgcca
    47221 tcaaggaagt gttcgcgtct ctttacaacg accgcgccat cgcctaccgg gtgcacaagg
    47281 gcttcgacca cgcgggcgtg gccctgtcgg ccggcgtgca gcgcatggtg cgttcggaca
    47341 tcggcgcttc tggcgtgatg ttcacgatgg acaccgagtc cggcttcaac ggtgtggtgt
    47401 tcatcaccgc ctcctatggc ctgggcgaga cggtggtgca gggcgcggtg aatcccgacg
    47461 agttctacgt gcataaggcc accctggccc tgggcaagcc cgcggtgatc cgccgtaacc
    47521 tgggctccaa gctcatcaag atggtgtttg ccgacaagaa ggaagccggc aagtccaccc
    47581 gcacggtcga tgtggcggaa gccgaacgca acgtgtattc cctcaccgac gaggacgtac
    47641 tggaactcgc ccgctacgcc accatcatcg agcagcacta cggtcgtccg atggacatcg
    47701 agtggggcaa gagtggcgac gacggcaagc tctacatcct gcaggcgcgt ccggagaccg
    47761 tgaagagcca gcagtccggc cacgtgatgg agaagtaccg cctcaagcag tacggcaagt
    47821 ccctcaccca cggtcgtgcc atcgggcaga agatcggcgc gggcccggtg cgtgtcgtca
    47881 aggacgcctc cgagatggag cgggtcaagg ccggcgacgt gctggtgacc gacatgaccg
    47941 acccgaactg ggagccggtg atgaagcgtg cctccgccat cgtcaccaac cgtggcggcc
    48001 gcacctgtca cgcggccatc atcgcccgcg agttgggcat ccccgccatt gtcggctgcg
    48061 gcaacgccac cgaggtgctg gacgagaacg acgaagtgac cgtctcctgc gccgaaggcg
    48121 acaccggcta cgtgtaccgg ggcaagctgg acttcgaggt ggtcaccacc gacatgggta
    48181 acctgccgga cattccggtg aagatcatga tgaacgtggg caatccggag ctggccttcg
    48241 agttcgccca gatccccaac ggcggcgtcg gcctggcccg cctggagttc gtcatcaaca
    48301 acatgatcgg catccacccc aaggcgatcc tcgagatcaa ccaggtgccg gccagcctgc
    48361 gcaacgagat caaccgccgc tcccgcggct acgccacgcc gcgccagttc ttcgtcgaga
    48421 agctggtcga aggcgtcgcc accatcgccg cagccttcta cccgaagccc gtcatcgtgc
    48481 gtctgtcgga cttcaagtcc aacgaatacc gcaagctgct cggcggcgag atctacgagc
    48541 cggaggaaga aaaccccatg ctgggcttcc gcggcgcgtc ccgctacatc gcccacagct
    48601 tccgcgattg cttcgagctg gaatgcgcgg cgatgaagaa ggtgcgcaac gaactgggcc
    48661 tcaccaacgt gcagctgatg gtgcctttcg tgcgcaacct ggacgaagcg gccggtgtgg
    48721 tggatctgct ggcgcggcat ggcctcaagc agggcgagaa cgacctcaag ctcatcatga
    48781 tgtgcgagat cccctccaac gcgctgctgg ccgatcagtt cctccagtac ttcgacggct
    48841 tctccatcgg ctccaacgac ctgacccagc tcaccctggg cctggaccgg gattccggcc
    48901 tggtggccca ttccttcgac gagcgcgatc cggcggtcaa gcagctgctg tccatggcca
    48961 tccagagcgc caacaagctc ggcaagtacg tgggcatctg cggtcagggc ccctcggacc
    49021 acgccgattt tgctgaatgg ctgatggatg aaggcatcca gaccatctcc ctgaatccgg
    49081 acacggtggt ggatacctgg ctgaagctgg cagcccattc caacaagggt tgaggcggac
    49141 gctggacggg ggagaccccg tcatgaaaga ggccggggtc tcccccggcc ttttttgttt
    49201 ccgttgcgca cctgctgcag ccgcgcccac cttccatgcg tttcagcggg ccgtggacaa
    49261 aaaaacggcg ccccgagggg cgccggaagg agggagaaac acaacag
//
LOCUS       DAOQMM010000014        46487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_181_length_46487_cov_51.394060, whole genome shotgun sequence.
ACCESSION   DAOQMM010000014 DAOQMM010000000
VERSION     DAOQMM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 46487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46487
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181_length_46487_cov_51.394060"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1521)
                     /locus_tag="PLH85_04045"
     CDS             complement(286..1521)
                     /locus_tag="PLH85_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HNB63760.1"
                     /translation="MALTDTAIKSAKPTDKPVKMTDGDGLYLLIHPNGGKWWRFDYRF
                     DSKRKTLSMGVYPDVSLRDARNRRDAARKQIANGIDPGEHRKAHKSARADAVANSFEA
                     VAREWYSKMRPTWTPDHADKIIKRFERDVFPWIGGKPVADLNAPEVLTTIRRIEARGR
                     LETAHRALQSCGQVFRYAVATGRAERDPTGDLRGALPPTREKHMAALTDPAEVGALLR
                     AVDAFRGTLIVQSALNLSALVFVRPGELRQAEWSEIDLDKGEWNIPASRMKGWTRKGI
                     TTPHLVPLAPQAVAILRELQPLTARGAHVFAGRDPKKPMSNAAVNAALRRMGYDTKTE
                     MTGHGFRAMARTILHEELGVDRDVIEHQLAHAVPDALGTAYNRTKFIKERRVMMTRWA
                     DYLDKLKVGAEVVTMRGAA"
     gene            complement(1685..2049)
                     /gene="ssrA"
                     /locus_tag="PLH85_04050"
     tmRNA           complement(1685..2049)
                     /gene="ssrA"
                     /locus_tag="PLH85_04050"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2105..2752)
                     /gene="gph"
                     /locus_tag="PLH85_04055"
     CDS             complement(2105..2752)
                     /gene="gph"
                     /locus_tag="PLH85_04055"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237005.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNB63761.1"
                     /translation="MADAVFFDLDGTLADTAPDLAVALNQLRAELGLPPCRFESLRPF
                     VSQGTRGMLSAGLGILPDHAEYAPLFDRFLSLYAGNLCVHTRLFTGMEVVLDALDERG
                     LPWGVVTNKAERFARPIITALGLDGRCACVVGGDTLTRRKPFPDPLLHAADLARVAPT
                     RSLYVGDDPRDITAGRAAGMQTVGVRYGYLGSNPAIDTWGADALISHPEELLAHL"
     gene            complement(2745..3440)
                     /gene="ubiG"
                     /locus_tag="PLH85_04060"
     CDS             complement(2745..3440)
                     /gene="ubiG"
                     /locus_tag="PLH85_04060"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNB63762.1"
                     /translation="MNADPAELQKFSELAHRWWDPESEFKPLHQINPLRLDWIDETVH
                     LAGKQVIDVGCGGGILSESMAARGAHVTGIDLSDKALGVARLHLYESGQTVDYRHISA
                     EDMARETPGQFDAVTCMEMLEHVPDPASTIRACAALVKPGGHVFFSTLNRNPKAYLMA
                     VVGAEYVLKLLPKGTHDYAKFIKPSELSRYVRNAGLDVEELIGMRYNPFTQIYALGRD
                     TDVNYLMRTRRNG"
     gene            complement(3571..4302)
                     /locus_tag="PLH85_04065"
     CDS             complement(3571..4302)
                     /locus_tag="PLH85_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB63763.1"
                     /translation="MIKQAKKQLLMAAALATLGLTASASFAKDVVVDTKGEVPYVIDG
                     RNVVARSGADLCWRTGYWTPAAASTTMAGEFPVGCECDKDVVAKEKCEPKAAAPAAGA
                     KGAAPIAAPAPVSEKVKLAADTLFDFDKAVLLPAGKTKLDDLADKAKGLNLEVILAVG
                     HTDRIGGDAYNQKLSEKRAAAVKDYLVSKGVAANRVYTEGKGKKQPVTGDKCKGLGKD
                     NGKNKKLVECLQPDRRVEIEVIGTK"
     gene            complement(4394..5725)
                     /locus_tag="PLH85_04070"
     CDS             complement(4394..5725)
                     /locus_tag="PLH85_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNB63764.1"
                     /translation="MAPVETVADIDLLIEARWIIPVQPAGLTLERHAVAIHEGRILAV
                     LPIDNARRQFRPREIRPLTDHILIPGLVNLHVHAAMTLMRGLADDLPLMRWLQEAIWP
                     AEGRHVSPGFVRDGSLLAAAEMLRGGITTCNDMYFFPEAAAEAFSRVGLRAMLGITVL
                     DFPTQYAADADDYLRKGLAARDAWRHDPLIGFTLAPHAPYTVSDRTFERVGGLAEELD
                     LPIHIHLHETATEVADSLRDHGLRPIARLDRLGLLGPGLIGVHAVHLSPEDIDRLATH
                     HCSIAHCPTSNMKLASGAAPMAAVAERGLRIGLGSDGTASNNRLDLFREMHQAALLAK
                     LTTGDAGALPAHRVLRMATLDGAAALGLDDRIGSIEPGKAADLCAVRLDDVDTLPCYD
                     PASHLIYVLGRESVSDVWVAGRQALKDGTLLQISNTELSSLARLWQNRLRQ"
     gene            complement(5797..6525)
                     /locus_tag="PLH85_04075"
     CDS             complement(5797..6525)
                     /locus_tag="PLH85_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB63765.1"
                     /translation="MNTAFRVASATLLALTGLTAQAQSKDIVVDPKGDVPYAIDQRNV
                     VVKSGADLCWRTGSWTPAAAGSVQAGELPVGCQCDRDVVGADICQPPAARGASGAPAA
                     AVAAPAAAVAAPRTCDFSVTLNADQSFAFGKATLSPAARRALDEVATRSAQCASVRAL
                     LITGHTDRLGTQAYNQKLSEKRAGAVSSYLAGKGLKAGDVEIMGAGKTQPIKGCDDSL
                     GHKKLVDCLAPNRRVTIDVKGPAK"
     gene            6756..9329
                     /gene="gyrA"
                     /locus_tag="PLH85_04080"
     CDS             6756..9329
                     /gene="gyrA"
                     /locus_tag="PLH85_04080"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNB63766.1"
                     /translation="MIPFAKETLPISLEDEMRHAYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELGNDWNKAYKKSARIVGDVIGKYHPHGDQSVYDTIVRMAQDFSLRYML
                     VDGQGNFGSVDGDGAAAMRYTEIRMAKIGHELLADIDKETVDFGPNYDGSEKEPLVLP
                     ARIPNLLINGSGGIAVGMATNIPPHNLGEVVDACLRLLQEPDVSIDELIKIVPAPDFP
                     TAALIYGLSGVHEGYRTGRGRVVMRARTHFEDIGKGDRQAIIVDELPYQVNKKALLEK
                     IAELVNDKRIEGISEIRDESDKSGMRVVIELKRNEMPDVVLNNLFKQTQLQDTFGMNM
                     VALVDGKPRLLDLRQMLECFLAHRREVITRRTVFELRKARERGHILEGLAVALSNVDE
                     IIALIKAAPTPPEAKKSLMARQWRSPLVEEMLARAASDSFRPTGLAPEFGLSAQGYRL
                     SDAQAQAILELRLQRLTGLEQDKIVAEYKEVMETIADLLDILARPERITAIIGDELAL
                     IKQQFGDPRRSEVVLNTADINIEDLIAPQDMVVTLSHTGYFKRQPLTDYRAQKRGGRG
                     KQAAAVKEDDFVDQLFVANTHDHILCFSNRGRLYWLKVYEVPEGNRNARGKPIVNLFP
                     LLEGEKITAVLPVKEFDEGHFVFMATAQGTVKKTPLTDFSNPRKAGIIAVALDEGDYL
                     VGVAITDGQHDVMLFSDAGKAVRFSENDVRPMGRTARGVRGMMLEDDQSVIALLVAGD
                     ESPSVLTATENGYGKRTPIAEYTRHGRGTKGMIAIQTSDRNGKVVAATLVTEKEQIML
                     ITTAGVLVRTRVSEIREMGRATQGVTLISVDEGSTLSGVQKIVESEEDEQDGEGPRAD
                     A"
     gene            9357..10442
                     /gene="serC"
                     /locus_tag="PLH85_04085"
     CDS             9357..10442
                     /gene="serC"
                     /locus_tag="PLH85_04085"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNB63767.1"
                     /translation="MRIYNFSAGPAALPTPVLEQVREELLDWHGAGMGIMEMSHRGKD
                     FMSVIAAAEADLRALMGIPDGYKVLFLQGGATQMFAQIPMNLLAGRSADYVVTGAWSK
                     KAWKEAPHIGPVRLAGTTEADGRFTRLPAADELSFDPDAAYVHLCTNETIHGVEMFDL
                     PATPAGVPLVADMSSHILSRPMDVSRYGLIYAGAQKNIGPAGLVLVIVRDDLLGRASA
                     ATPSVMNFATMAEHGSMLNTPPTFGIYLAGLIFKDLLRQGGLAAVEAANRAKAERLYD
                     AIDGSGGFYRNPVDRPCRSLMNVPFTLADPALEADFLAEAAARRLAGLKGHKSVGGMR
                     ASIYNAMTLDGVEALVDLMQDFARRKA"
     gene            10478..11557
                     /gene="pheA"
                     /locus_tag="PLH85_04090"
     CDS             10478..11557
                     /gene="pheA"
                     /locus_tag="PLH85_04090"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNB63768.1"
                     /translation="MLQELGAVRDQIDALDAQMLALLNARARCAQHVGEIKARHGEAG
                     QVYRPEREAQVLRRIQALNAGPLPDESVTFFFREVMSACLSLENPLGVAFLGPLGTFS
                     ESAAVKHFGHAARLAPQTTIDDVFREVESGHAQYGVAPVENSTEGAVGRTLDLLLSSP
                     LQICGEVVLRIHQHLMSGETALGDVRKVYSHAQSLAQCHEWLNSHLPGVPRISVASNA
                     EAARLAASEPGAAAIAGEAAAERYALPTLARSIEDEPNNTTRFLVLGRHDAGPSGADK
                     TSLIMSAANRPGAMHALLAPLADAGVSMTRLESRPARAALWEYVFYVDFAGHRNDPEV
                     RKALDALSAHASFLKILGSYPQAVY"
     gene            11574..12671
                     /gene="hisC"
                     /locus_tag="PLH85_04095"
     CDS             11574..12671
                     /gene="hisC"
                     /locus_tag="PLH85_04095"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNB63769.1"
                     /translation="MRLCDLSPAHIRAISPYQPGKPIAELAREMGLAESSIVKLASNE
                     NPLGFGDKARAAMAAALDDLARYPDGNGFELKQALCRRYGVAMDEIVLGNGSNDVLEL
                     AARAFLGPQTSAIYAQHAFAVYPLATQAVGARGVEVPARHFGHDLDAMLAAITPDTRV
                     LFIANPNNPTGTFVPGPELEAFLVRVPDHVLVVLDEAYTEYLGEEQRYDAIAWLARFP
                     NLLVCRTFSKAYGLAGLRVGYGLGHPAVIDLLNRVRQPFNVGSVALAAAAAALFDSDF
                     LARSYEVNRAGMAQLTAGFARLGLEWIPSAGNFVTFRAGDGAGVNRRLLRQGVIVRPI
                     AGYGMPEWLRVSIGLEAENARFLEALPQALG"
     gene            12682..13575
                     /locus_tag="PLH85_04100"
     CDS             12682..13575
                     /locus_tag="PLH85_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNB63770.1"
                     /translation="MRKIDKLVICGVGLIGGSVALALRQARLVNRIVGVGRRRETLEQ
                     ALQLGVIDEISTDWAEALDGAEIVLLAMPVGQTDAVAAAMAPHLGPDTLVTDAGSTKR
                     DVIEAIYRHLAAHLPRVVPAHPIAGAEKSGPSAARADLYRKRKVVVTPLPENDAVAVA
                     RVRELWAACGAHLFEMTPQEHDRVFAAVSHLPHLLAFGLVHDLAGRANAEELFSYAAS
                     GFRDFTRVAGSHPEMWRDICLANRHSLLAELDAYLGELAWLRALLASGDGPALEKVFS
                     EASRARNEWADKAFPATPSGE"
     gene            13590..15551
                     /locus_tag="PLH85_04105"
     CDS             13590..15551
                     /locus_tag="PLH85_04105"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HNB63771.1"
                     /translation="MEFIDLPPMLRAVGEVRLPGSKSISNRVLLLAALAEGTTRVEAL
                     LDSDDTRVMRDALVALGVGLQQEGDTLVVTGCGGRFPNARADLFVGNSGLSIRTLVPA
                     VVASLAGCGDADAAVRLSGVPRMHERPIGDLVDGLAQLGARVDWLGQPGFPPLALRPA
                     PLAAETVSVRGNVSSQFLTGLLQAAPLVARDTPLVIVVEGELISRPYVEITLNLMARF
                     GVQVERDGWSRFVVPTGQHYVSPGRIVVEGDASTASYFLAAGALGGGPLRVKGAGRGS
                     IQGDVRFADALAEMGAQITWGEDWIEARAPESGRLKGIDLDLNHIPDAAMTLAVAALV
                     ADGPSHLRNIGSWRVKETDRIAAMATELRKLGATVEEFPESLRITPPARLQPAVIDTY
                     EDHRVAMCFSLATLCGTSLRINDPKCVNKTFPGYFETFAGITAPVPVVAIDGPSASGK
                     GTVAARVAQALGFAHLDSGSLYRIVALTAIRRGVALDDEPTLAAIAADLPARFEGDRV
                     MLEGDDVTEAIRSEACSVGASRVAALPTVREALFWRQRAYRRAPGLVGEGRDMGSVVF
                     PDAPVKIFLTASVEARAERRYKQLMDKGMPANMADLLKDLRERDARDAQRAAAPLKQE
                     EDAVLLDTSDMSVEQAVAFVLERAAILKG"
     gene            15647..17353
                     /gene="rpsA"
                     /locus_tag="PLH85_04110"
     CDS             15647..17353
                     /gene="rpsA"
                     /locus_tag="PLH85_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNB63772.1"
                     /translation="MSIATPVSIEESFAALFEESLNRQEMRAGEVITAEVVRIDQNFV
                     VVNAGLKSESYIPLDEFRNDRGELTANVGDYVQVAIEQLEDGYGETRLSRDKAKRIAA
                     WNDLDKALNEGILVKGVITGKVKGGLTVMVNGIRAFLPGSLVDMRPVKDTTPFEGKEF
                     EFKVIKLDRKRNNVVVSRRAVLEASMGEERQKLLETLKEGTVVKGVVKNITDYGAFVD
                     LGGIDGLLHITDLAWRRVRHPSEVLSVGDELEAKVLKFDQEKNRVSLGLKQLGEDPWV
                     GISRRYPQGTRLFGKVTNITDYGAFVEVEQGIEGLVHVSEMDWTNKNIHPTKVVQLGD
                     EVEVMILEIDEDRRRISLGMKQCMSNPWDDFAINHKKGDKVRGQIKSLTDFGVFIGLE
                     GGIDGLVHLSDLSWSLAGEEAVRQYKKGDEVEAVVLNIDVEKERISLGIKQLEGDPFT
                     NFIATHDKNTLVTGTVKSVDARGAVVALNDDVEGYLRASEFSRDRVDDLSQLLKVGDA
                     VEALIVNVDRKTRGINLSVRAKDQVEQAEAMNKLASDSGSAASGTTNLGALLKAKLNE
                     QK"
     gene            17365..17649
                     /locus_tag="PLH85_04115"
     CDS             17365..17649
                     /locus_tag="PLH85_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNB63773.1"
                     /translation="MTKSELIARLAERFPQLVAKDAETAVKMILDAMTDALSRGDRIE
                     IRGFGSFALNYRPPRVGRNPKSGEKVQVPEKYVPHFKAGKELRERVDMIV"
     gene            17742..18047
                     /locus_tag="PLH85_04120"
     CDS             17742..18047
                     /locus_tag="PLH85_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNB63774.1"
                     /translation="MRLLTWIVRLFLFLVLFGFAVKNDDVVTLRFFFGAQWQVPLVLV
                     ILLFVVIGVLIGVTAAVATLYRQHRELGQLRERQADAPPPVTVRPPVAHGPDLPETY"
     gene            18054..19229
                     /gene="lapB"
                     /locus_tag="PLH85_04125"
     CDS             18054..19229
                     /gene="lapB"
                     /locus_tag="PLH85_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HNB63775.1"
                     /translation="MEFELWWLLAFPLFFALGWLAARIDIKHLVKESRALPRTYLTGL
                     NFLLNEQPDKAIDAFIEAVKIDNQTVELHFALGSLFRRRGETDRAIRMHQNLIDRDDL
                     SQDQRLQALSELGLDYLKAGLLDRAEAVFVQLRGSRLDEFALKNLLDIYQQEKDWRKA
                     IDIAQALPGHESALWEKEVANFHCELAAAAIADSRFDEARSNLERALETNRKSVRASI
                     LLGDLHAAQGDDEAALEAWKRVETQNPVYLALVAERIMDAYRRLGRLEQGMQLLRSYL
                     DSYASLDLLDAVFQWELQKEGAQAAYHLVREELRRNPTLLGLDKLLEAALLTAPVEQR
                     TDIELVKGLVHGHTRRVARYRCDACGFKARQFYWRCPACGGWETYPPRRTEEFDLTP"
     gene            19432..20322
                     /gene="cysM"
                     /locus_tag="PLH85_04130"
     CDS             19432..20322
                     /gene="cysM"
                     /locus_tag="PLH85_04130"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNB63776.1"
                     /translation="MEFKTLEDYVGQTPLVRLKRITAGRNNTILAKLEGNNPAGSVKD
                     RPALSMILHAEARGDIRPGQTLIEATSGNTGIALAMAAAMRGYGMILVMPENQSIERR
                     QTMRAFGAELVLTPKEGGMELARDVAERMRDEGRGVILDQFANPDNPLAHIEGTGPEI
                     WDQTGGTVTHFVSSMGTTGTIMGVSTYLKARNPAIRIIGCQPADGAQIPGIRKWPEAY
                     LPRIYDKARVDQLEYVAQADAEEMTRRLAREEGIFAGISSGGALHVALRLAAQLEDAV
                     IVSIVCDRGDRYLSTGVFPA"
     gene            20329..21126
                     /locus_tag="PLH85_04135"
     CDS             20329..21126
                     /locus_tag="PLH85_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB63777.1"
                     /translation="MVPILVFDIETIPDVAGIRALNGLPAELGDDEVAEFAFQQRRAA
                     TGNDFLPHHLQRVVTISCVLRDDSQFRVFSLSEPDATEGQIIQRFFDGVEKFTPQLVS
                     WNGGGFDLPVLHYRGMMHGVAASRYWDLGDGDYFDSRDFKWNNYIGRYHTRHLDLMDL
                     LALYQPRASAPLDDLAKLMGFPGKLGMDGSAVWQGYRDGRIAEIRDYCETDVVNTYLV
                     LLRFQRMRGILSAERYEEEIAFVRKSLSSLPARHWQRFLEAWGDGSS"
     gene            21187..21263
                     /locus_tag="PLH85_04140"
     tRNA            21187..21263
                     /locus_tag="PLH85_04140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21221..21223,aa:Val,seq:gac)
     gene            21401..23317
                     /gene="thrS"
                     /locus_tag="PLH85_04145"
     CDS             21401..23317
                     /gene="thrS"
                     /locus_tag="PLH85_04145"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNB63778.1"
                     /translation="MPNITLPDGSVRSFDQPVTIAQVAASIGAGLARAALAGKVDGRL
                     VDTSFLIDRDVQLAIVTDKDADGLELIRHSTAHLLAYAVKALFPDAQVTIGPVIENGF
                     YYDFSYTRAFTPEDLAKIEARMLELAKKDIPVERSVLPRDEAVAYFKSIGEHYKAEII
                     ASIPANEDVSLYKEGDFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSRNEMLQRIY
                     GTAWAKKEDQDAYLHMLEEAEKRDHRRLGRQLDLFHLQDEAPGMVFWHPHGWTLWQQV
                     EQYMRSVLNEAGYQEVKTPLVMDRALWEKSGHWDNYRDNMFTTESEKRDYAIKPMNCP
                     GHVQIYNHGLHSYRDLPLRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEEQI
                     VSEVTAFNELLTRVYRDFGFTDVAVKLSLRPEKRAGTDAIWDKAETGLRQALAASGHS
                     WEELPGEGAFYGPKIEYHIKDALGRSWQCGTMQLDFVLPERLGAEYVAEDNARYRPVM
                     LHRAILGSLERFIGILIENHAGALPLWLSPVQAVVMNISEAQADYVTEVVNRLRSRGF
                     RVEADLRNEKITYKIREHSVRKLPYLLVIGDKEKAADVVAVRARGNQDLGQMSLEQVV
                     ERWQRELLARAGTA"
     gene            23364..23867
                     /gene="infC"
                     /locus_tag="PLH85_04150"
     CDS             23364..23867
                     /gene="infC"
                     /locus_tag="PLH85_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNB63779.1"
                     /translation="MRINGEITAPEVRLTGPDGEQIGIVSLRAALEAADEAGVDLVEI
                     APMAKPPVCRLMDFGKYKYEEAKRAHEAKQKQKQIQVKEVKLRPGTDENDYQIKLRNL
                     IKFLNEGDKAKVTLRFRGREMAHQEFGMRQLERIKADLEEIAQVEQMPKMEGRQMIMI
                     IAPKRKH"
     gene            24010..24207
                     /gene="rpmI"
                     /locus_tag="PLH85_04155"
     CDS             24010..24207
                     /gene="rpmI"
                     /locus_tag="PLH85_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNB63780.1"
                     /translation="MPKMKTKSGAAKRFKVRSSGSIKRSQAFKRHILTKKTTKSKRHL
                     RGMTCVHASDEKSIRAMMPYA"
     gene            24222..24581
                     /gene="rplT"
                     /locus_tag="PLH85_04160"
     CDS             24222..24581
                     /gene="rplT"
                     /locus_tag="PLH85_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNB63781.1"
                     /translation="MPRVKRGVTARARHKKVLDQAKGYRGRRKNVYRIAKQAVMKAGQ
                     YAYRDRRQRKRQFRALWIARINAASRELGLTYSRFMNGLKKAAIEVDRKVLADLAVFD
                     KPAFAALAEQAKAKLAA"
     gene            24652..25683
                     /gene="pheS"
                     /locus_tag="PLH85_04165"
     CDS             24652..25683
                     /gene="pheS"
                     /locus_tag="PLH85_04165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNB63782.1"
                     /translation="MDQLDQLVAQAGADFAAAADSAALETAKARFLGKGGSITELLKG
                     LGKLDAEARKAAGAEINKAKSAIEAALEARREALRQAALEAQLAAEALDVTLPGRGAG
                     LGGLHPVVRTLERVEQLFRSIGFEVADGPEIETDFYNFTALNTPANHPARSMHDTFYL
                     ENGEGVLLRTHTSPVQIRAMQAHVDAYKDRDGMPELRVIAPGRVYRVDSDATHSPMFH
                     QVEGLWVGESVSFADLKGVIADFLRRFFESDDLQVRFRPSFFPFTEPSAEIDVAFMSG
                     ALKGRWLEIAGCGMVHPNVLRFGGIDPERYTGFAFGMGPDRLTMLRYGVNDLRLFFEG
                     DLRFLGQFR"
     gene            25687..28065
                     /gene="pheT"
                     /locus_tag="PLH85_04170"
     CDS             25687..28065
                     /gene="pheT"
                     /locus_tag="PLH85_04170"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNB63783.1"
                     /translation="MQFSELWLRSFVSPALDADALGHLLTMAGLEVEEQGGVAAAFSK
                     VVVARILEADKHPNADKLRVCKVDAGQGEPLQIVCGAPNAAAGLTIPCALVGAELPGF
                     AIKAAKLRGVESFGMLCSAKELGIADDSSGLLVLPEDAPVGTDLRDYLALDDTVYTIK
                     LTPNRADCLSLTGIAREVAALTDRPLSLVDTSSVAPTIADTRKVVLDAPEACPRYCGR
                     VVRGVNAQAPTPDWMKQRLARSGIRSISVLVDVTNYVMLELGQPLHAFDNARLDGAIH
                     VRMPKAGEQVLLLNGQTVTPAADTLLIADESRALALAGIMGGEESGITLETTDLFLES
                     AFFAPDAIAGRARAYGFSSDASYRFERGVDFALAPAALERATRLILDLCGGEAGPVVE
                     ALAADRLPQRAPIRLRAARVRRVLGIDVSADEVADLFGRLNLAVNRDGDDFVVTPPSY
                     RFDLEIEEDLIEEIARLHGYDNIPTVVPKGPIAMLPRPEARRDPMGVRRLVAAREYQE
                     VVNFSFVEEAWERDFCGNADPVRLANPIASQMSVMRSSLIGGLVANVVTNRKRQNNRV
                     RVFELGRVFRRDAAGAPVEGFHQPVRVAALAAGPLRPEQWGEAVRTVDFFDVKADLEA
                     LLHPRVATFEPVVHPALHPGRAARVLLEGQEIGVIGELHPVWVQRYELGTAPVVFEVS
                     LTSLLETMTPAYREVSRFPAVTRDLALVVDQATPVASLIAGLSSVAPAIVQRIELFDQ
                     YQGKGIEPNRKSLAFRVVMQETQRTLEDAEVEAAMTALTRHAEEALGAKLRG"
     gene            28071..28376
                     /locus_tag="PLH85_04175"
     CDS             28071..28376
                     /locus_tag="PLH85_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNB63784.1"
                     /translation="MGVTLTKAELADMLFEQVGLNKREAKDMVECFFEEIRLALERGD
                     CVKLSGFGNFQLREKPQRPGRNPKTGEEIPITARRVVTFHASQKLKANVEHLSDVAK"
     gene            28363..28740
                     /locus_tag="PLH85_04180"
     CDS             28363..28740
                     /locus_tag="PLH85_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB63785.1"
                     /translation="MSQSEVTEFVELPPIPAKRYFTIGEVSELCAVKPHVLRYWEQEF
                     TQLRPVKRGGNRRYYQHHEVLLIRRIRELLYRDGFTISGARNRLDEMASRKREDVEES
                     AVLRDQLASIRGEIQAILKDLQG"
     gene            complement(28923..29816)
                     /gene="rsgA"
                     /locus_tag="PLH85_04185"
     CDS             complement(28923..29816)
                     /gene="rsgA"
                     /locus_tag="PLH85_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNB63786.1"
                     /translation="MKARGTLVAAFGRQYEVRTDGGEILLCVPRGKKSRFACGDRVQV
                     ARSAPGQGVIEQLEERGSLLYRSDAWRQKLIAANATQVVVVVATEPSFSDDFVTRCIC
                     AAESQQLKSLIVLNKADLPAGLDAARTLLAPFAALDYPILELSALHDVDALRARLKDE
                     TSLLVGQSGMGKSTLTNALIPEARAETREISEVLDTGKHTTTFARLYDLPGGGGLIDS
                     PGMQTFGLAHLDNAALEASFREFRPWDGQCRFRDCRHDSEPGCALRAAVADGRIAPRR
                     LTQFLELRAECEQARKTAQGW"
     gene            complement(29813..31066)
                     /locus_tag="PLH85_04190"
     CDS             complement(29813..31066)
                     /locus_tag="PLH85_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB63787.1"
                     /translation="MSTSSFTTLFLVAVLAGLAVRIPLALRHARHVSAHRNAVPPAFA
                     TAIGLDAHRKAADYTVARVHLGLVDLLVSTVWLLILTLGGGLQALFDLSAGVLDAGSL
                     GQGVAFLTLVAVASWLVDLPLGLYRSFVLEARFGFNRLTPRLFVADTLKGALLAALIG
                     LPLLAGALWLMGAMGERWWLWVWAAWLGFNLLAMLIYPSFIAPLFNRFQPLEDGDLKT
                     RIEALMTRCGFRLSGLFVMDGSKRSAHGNAYFTGFGGSRRIVFFDTLLEKLDPAEVEA
                     VLAHELGHYHHHHLWKRLAVIGTGSLAFFALLGALAQQGWFFTGLGMTATGAAPTLVL
                     FSLALPVFTFPLTPLMSHWSRRHEFEADAYAARQAPAASLISALVKLYRDNASTLTPD
                     PLYSRVYDSHPPAAVRIARLEAAVR"
     gene            31241..31786
                     /gene="orn"
                     /locus_tag="PLH85_04195"
     CDS             31241..31786
                     /gene="orn"
                     /locus_tag="PLH85_04195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNB63788.1"
                     /translation="MAQDPNHLIWLDMEMTGLNPDTDRIIEIAMIVTDSQLNLVAEGP
                     VVAVHQPDAVLEAMDDWNKNTHGRSGLIDRVKASTVDEAAAEALFLDFLQAHVPARTS
                     PMCGNSIGQDRRFMVRYMPKLEDFFHYRNLDVSTLKELCKRWRPDVYAGLTKQGAHQA
                     LADIQESIDELRYYRANFLKV"
     gene            31897..32040
                     /locus_tag="PLH85_04200"
     CDS             31897..32040
                     /locus_tag="PLH85_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63789.1"
                     /translation="MSRDSLRETWRKHRRMVIGVAVLCAFFYALGYFSPRVASPLMCK
                     IYG"
     gene            complement(31996..33012)
                     /gene="zapE"
                     /locus_tag="PLH85_04205"
     CDS             complement(31996..33012)
                     /gene="zapE"
                     /locus_tag="PLH85_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNB63790.1"
                     /translation="MTPLRVALEARFRRGGPIPDTAQGAALDHLARLADALVHRRATR
                     GLFLWGGVGRGKTRLMACLDAVLPPAAIRRYPLNALLGAYHATCVCQPDWAARLDTLF
                     GSARVLLLDEFHAYDPADALVLERLLAGVAERGMVCVVTANHPPDRLWPDTPEHRARR
                     HHFAPVAQRLAAQCLLLEVDGGRDYRELLAAHGPLRWWSATDATDRQAARRWLTATAG
                     CARDFDELFRQPRPQRDYVRLAETAGGLLIDGFPRLTPRDGDALRRLVWCLDAAWDAG
                     LALGVLAEAAPETWVAGIGAELDGLLGKDLARARSRLRALCRVDGAGVSAVDLAHEGR
                     RDPR"
     gene            complement(33009..33764)
                     /locus_tag="PLH85_04210"
     CDS             complement(33009..33764)
                     /locus_tag="PLH85_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB63791.1"
                     /translation="MALLPDVPLGDAAGVACAAAILVIAYALFALAGFGSALIASAPL
                     AWVLPVARIVPLLAVLDCAGSVSRAWQARTTVDRAALGRLLPGMLLGQIVGVGLLSAL
                     PAHGLALLLGGFVAGYGVWHALGRRGAEQATPTRAWLYGGFGGVLGGVFGSGGFAYAA
                     YLQPRLERAAFRATQGVLIALSTAWRIALCAASGLVDGRLLVTAALLLPAAWLGLTLG
                     QRIDRRLSGPALGRLIDGLLIVAGLALAGKALA"
     gene            33869..34780
                     /locus_tag="PLH85_04215"
     CDS             33869..34780
                     /locus_tag="PLH85_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB63792.1"
                     /translation="MLSLRQIRYFQAVADTGSFTLAAAQVYIAQPALSRQIALLEAEL
                     GVALFVREARGVRLTPAGQLFRARTENVGTALEAAVEDARRLDRGEAGVLRLLHSSSV
                     PLAGPLLEALRCLTDAAPAARVELDRVSSEVQVAEIAAGRADVGVARMPVLRREAAVA
                     FTALPEERLVLAVPGAHPLAGRTAVSLAELAGETFVTAVHRERGGLARRVTDLCLARG
                     FVPRSGAVVSRKTSMLNLVAAGYGVAVVPAGMAGLLPAGARLVALTDAEATSASAVLL
                     PLAPSPLARRFAELCGAAWAGVAGAGC"
     gene            complement(34777..35415)
                     /locus_tag="PLH85_04220"
     CDS             complement(34777..35415)
                     /locus_tag="PLH85_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB63793.1"
                     /translation="MNWLTRFLPGGGGTPLTPDQKAALDAVAALPAPDLGRVHFETRY
                     VAVNAETGPVEDGGRRLLAVGAVALNRGLIHPADAYYARLGDTPADALIGLLAFIARA
                     PVVVFNAPFNRGSLERAFEQHLGPLPELAWIDLMVMLPSLYPERIGGQARMDAWLGSF
                     GIDRYDSRDALLESLVVAQLHQVALAATRHFNLPTPRDLLDTESSRRWLRGG"
     gene            complement(35412..37322)
                     /locus_tag="PLH85_04225"
     CDS             complement(35412..37322)
                     /locus_tag="PLH85_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNB63794.1"
                     /translation="MDTPSASDTLIRTQLDFLRRHAPFDRMEPDALRFLGERLSTAYY
                     PAEAEVLMPEDGPPRFFYIVYRGKVQARQVGVTVTEYSTLTLGPGEGFPIGAISAERP
                     STNAYVALEDSYCFQLPAADFLKLLEISPSFQLFCTRYIASLLNQSREQLQVQFAQRA
                     AEQQTMTTSLAQLATKAPVFVGPETPTRSALEKMVEAGVGCLAVAGEDEKPVGVLTQS
                     DILKRIVLPGFDLTRPIGDVMTRPPHTLHATASAYDAALEMATHGVRHILVVDTEDKL
                     KGVISERDLFALQRIGLRQIRSGIESAADIEALQRASRDIRQLALNLLAQGIGAEQLT
                     QFISALNDALTRRIVELNLQRHDLEGIEYAWLAFGSEGRHEQTLSTDQDNGILFTLPE
                     WADKEPLKLRLLAFAKDVNNDLAACGFPLCEGNIMASNPELCLTFDEWREKFGQWIRE
                     PHPEALLNATIFFDYRVLYGDEKLAHKLRQWLLSLTGGNPAFLKSLATNALDVAPPLG
                     KIRDFVVDDDPKYPGTIDLKKFGARLFVDAARVLALRTGVEATATVQRLRQGGSRIGM
                     PAEELSAMADGFHFIQLLRLRHQHLDTDHGAPGDNRVKPDDLNELDRRILKEAFRQAR
                     KLQLRLKLDYQI"
     gene            37467..38141
                     /locus_tag="PLH85_04230"
     CDS             37467..38141
                     /locus_tag="PLH85_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB63795.1"
                     /translation="MNAALLPVVEIEPAVPATRSVIWLHGLGADGHDFEPVVDEFDLD
                     RLPPTRFVFPHAPMRAVTINGGYVMRAWYDILSMDFSERREDPAGVLESAAQVEALIA
                     RENARGIADGHIVLAGFSQGGAIALHTALRHPRRLAGVLALSTYVPLADTLAAEAAPA
                     NRDVPLFMAHGLGDPVIPYAFSRRSGDLLADLGYPLEWHDYPMDHALCLEEVRHIEDW
                     LARVLA"
     gene            complement(38160..38612)
                     /locus_tag="PLH85_04235"
     CDS             complement(38160..38612)
                     /locus_tag="PLH85_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNB63796.1"
                     /translation="MDTRFLDDLNPGDRFTSPGITLTEAEIIDFALRYDPQSFHLDAV
                     AAAESPYGGLIASGFQSLALCFRLFIQSGILAESSMGSPGIDELRWLAPVRPGDTLHT
                     EVDVLEVRPSSTRPDRGIARFKYTAVNQRGETVLSFIVAHLLRRRPAG"
     gene            complement(38643..39542)
                     /locus_tag="PLH85_04240"
     CDS             complement(38643..39542)
                     /locus_tag="PLH85_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB63797.1"
                     /translation="MDLRALRYFIETVRHGSFTVAAERLCVTQSTVSKMLRQLEDEVG
                     QPLLHRDGRRIRVTDVGRILFDRGQEALGVMRELNREVADLTALARGELTVGLPPTVN
                     LFFTPVVKAFRERHPGVRLTLVERGGQIIEQQVASGELEVGVTLLPVDPALGLATRTV
                     GRHPIVAVGPADALWARQAQVDLSHLDGEALVLLHDDFALTRQLRDAFRRFGVEPRIV
                     AQSGQWDFLVALAAAGLGTALLPQPLLARMALHPELAVRPLADPALAWTVAHIWPPGR
                     YLSHAARAWLAVCEDVLDGGAIG"
     gene            39666..41180
                     /locus_tag="PLH85_04245"
     CDS             39666..41180
                     /locus_tag="PLH85_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HNB63798.1"
                     /translation="MPLDRIRLPRLRERIMSPEEAASLFRDGMTVGMSGFTRAGDCKA
                     VPFALAERAAREPLQITLMTGASLGNDIDKVLSEANAIARRIPFQSDPSLRRKINAGE
                     VMFIDQHLSETVEQLRSGQIAPVDIAVVEASAITENGGIVPTTSVGNSASFAVLAKKI
                     IVEINLKMPVELEGLHDIYIPTYRPHRLPIPLIAPEQRIGLPYIPVDPEKIVAIVITE
                     KADSPSNVLPPDPETNAIAGHLNEFLYHEVKAGRLSPSLQPLQAGIGTIANAVLHGML
                     DSPFRDLKMYSEVLQDSTVELLDDGRLSFASASSITLSKATYERVLGQIERYKQRLVL
                     RPQEISNHPEVIRRLGLITINTALECDLYGNVNSTHVGGTHMMNGIGGSGDFARNAHL
                     SVFVTKSIAKDGAISSIVPMVSHTDHTEHDTDIIVTEHGLADLRGLAPRERARQIIDH
                     CTDPLYRDALRDYFLRACRRGGQTPHLLEEAFSWHLRYAREGAMLARDGRVEPA"
     gene            complement(41333..42382)
                     /gene="selD"
                     /locus_tag="PLH85_04250"
     CDS             complement(41333..42382)
                     /gene="selD"
                     /locus_tag="PLH85_04250"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNB63799.1"
                     /translation="MTDTPLRLTGLSHGGGCGCKIAPGVLTEMLARMPAAQPFANLLV
                     GTETADDAAVWRLNDTQALIATTDFFMPIVDDPFDFGRIAATNALSDVYAMGGTPILA
                     LAIVGMPLDKLPLDAIGRILAGGASVCADAGIPVAGGHSIDAPEPIYGLVALGLAHPD
                     VIKRNRDARAGDVLVLGKPLGVGILGSAMKKGLLDADGYAQMIATTTRLNTPGPVLAQ
                     LPGVHALTDVTGFGLLGHLLELCRGASLGATVEAARLPVMPAAVPLAQQGIGPGAIAR
                     NLASFGDAVRFDEDVADWQRHLVADAQTSGGLLVSCAAEAVPAVLEVFRQHGFAEAAV
                     IGAMHDGAAEVRVRR"
     gene            42414..43475
                     /gene="mnmH"
                     /locus_tag="PLH85_04255"
     CDS             42414..43475
                     /gene="mnmH"
                     /locus_tag="PLH85_04255"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNB63800.1"
                     /translation="MSVSPHAPLSVGLDRLAHFDAVIDVRSPSEFAEDHIPGALNCPV
                     LDDEERRRVGTLYKQVSPFEARKLGAALVSRNIARHLETHFLSKPKGWKPLVCCWRGG
                     QRSGAMTLVLNQVGWGARQLAGGYKAFRQQVMDDLVALPEGLSFRILCGPTGSGKTAL
                     LRRLAAQGGQVLDLEGLAVHKGSVLGGDVVRPQPGQKAFETGLWQALRGFDPARPVWV
                     EAESRRIGRLHLPPELFARLADGRCTLVRAPLEARVAYLLRDYADLIAEPAAFGARLE
                     RLIPLHGHERVLAWRAQIDGGDWETLARELIVTHYDPAYRRGGDGLYRHVAQAAVLDA
                     ASLDADALDALARGLANAI"
     gene            complement(43540..44949)
                     /locus_tag="PLH85_04260"
     CDS             complement(43540..44949)
                     /locus_tag="PLH85_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB63801.1"
                     /translation="MATRRTTKKAAITKLFVLDTNVLMHDPTSLFRFEEHDIYVPMMT
                     LEELDSNKKGMSEVARNARQASRSLDEIVNSSPDGIEAGISLEQASSQLASGRLYLQT
                     EAIDTRLPALPTAKGDNQILAVVMHLHEKFAKRPVILVSKDINMRIKARALGLAAQDY
                     FNDKVLEDTDLLYTGVKEIDAPFWERNGKDMESWKQEGRAYYRIKGPDTADLLINEFV
                     FQEGEQPLQAWVKDKAGRSITLESLTDYSHQKNNVWGITARNREQNFALNLLMNPEID
                     FVTLLGQAGTGKTLLTLAASLTQVLESKRYSEIIMTRVTVPVGEDIGFLPGTEEEKMA
                     PWMGALEDNLDVLNGSTGEGGEWGRAATRDLIRSRIKIKSLNFMRGRTFINKFLIIDE
                     AQNLTPKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRFKGWPHSGH
                     ITLQRGERSRLADHAAEVL"
     gene            complement(44974..45441)
                     /locus_tag="PLH85_04265"
     CDS             complement(44974..45441)
                     /locus_tag="PLH85_04265"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNB63802.1"
                     /translation="MLDQAAPDFSLPATGGQTLSLAQFRGRSVVLYFYPKDSTPGCTT
                     ESQQFRDLHDQFLAAGAVVLGLSRDSLKSHDNFRAKQALPFDLISDPDETACTLFGVM
                     KLKNMYGKQVRGIERSTFVIDAQGVLTREWRGVKVPGHAQEVLDYVKTLSTAA"
     gene            complement(45532..45909)
                     /locus_tag="PLH85_04270"
     CDS             complement(45532..45909)
                     /locus_tag="PLH85_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNB63803.1"
                     /translation="MKLHLDKAEQYNTISRYDATHVRVGAERYEGSVIVLPDRIEAGW
                     HMGGFDALDEAAFARLAGLGTEIVILGTGLRQRFPAPQLLRPLMQARIGFEVMDLGSA
                     CRTYNLLVAEHRAVAAALLFDPA"
     gene            46124..>46487
                     /locus_tag="PLH85_04275"
     CDS             46124..>46487
                     /locus_tag="PLH85_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNB63804.1"
                     /translation="MQPILKSDKLANVCYDIRGPVLQKAKQMEDEGHKIIKLNIGNLA
                     AFGFDAPEEIQLDMIRNLPNAAGYSDSKGIFAARKAIMHYTQQKHIKGVTLEDIYVGN
                     GVSELIVMAMNALLNAGDE"
BASE COUNT         7760 a        14970 c        15966 g         7791 t
ORIGIN      
        1 gtggactcct tgttcctgaa ggatgaggtg tccacggaaa tggggcaact tcagtcatgg
       61 cttggtaaca accccacgcg taggccaagt cgagagctaa ccgacgtcgg gtttcatcag
      121 ggagatctgg catcgcccgg agtgcaacgc ctagggcttt tcctaccgtt ttcatatcga
      181 tagtcgacac cggagccggt tggcgctttc tcgggcgacc cttaccaggt gtaaccaccg
      241 gatcaggcca cgggccacac ggatagtgtc agtcttctcc gctgctcatg ccgcgcctcg
      301 catggtcact acttctgcgc ctaccttgag tttgtccaga tagtcggccc atcgggtcat
      361 catcacccgg cgttccttga tgaacttcgt ccggttgtag gccgtgccca aggcgtccgg
      421 gaccgcgtgt gcaagctggt gctctatcac atcccgatcc acgcccagct cttcatgtag
      481 gatcgtccgg gccatcgccc ggaagccgtg gccggtcatc tcggtctttg tgtcgtagcc
      541 catgcggcgc agggcggcat tcacggccgc gttgctcatt ggcttcttcg gatcgcggcc
      601 ggcgaacacg tgcgcgcctc gcgcggtgag tggttgcagc tcgcgcagga tcgccaccgc
      661 ttgcggcgca aggggcacga ggtgcggcgt cgtgatgcct ttgcgcgtcc agcccttcat
      721 ccggctggcg gggatgttcc actcgccctt gtccagatct atctccgacc attcggcttg
      781 tcgcaactcg ccggggcgga cgaacaccag cgccgacaga ttcagggcag attgcacgat
      841 cagggtgccc cggaaggcat cgacggcgcg gagcagggcg cccacctcgg cggggtcggt
      901 cagggcagcc atgtgctttt ccctggtcgg gggcagggcg ccgcgtagat cgcccgtagg
      961 atcgcgttcg gcgcgtccgg tggctacggc atatcggaac acctgcccgc agctttgaag
     1021 ggcacggtga gctgtttcca gccgtccgcg ggcctcgatc cggcgaatgg tggtcagcac
     1081 ctcgggggcg ttcaggtcgg cgaccggctt gccgccgatc caagggaaca cgtcccgctc
     1141 gaagcgcttg atgatcttgt cggcgtggtc gggcgtccag gtgggccgca tcttgctgta
     1201 ccactcgcgg gcgacagcct cgaagctgtt ggcgactgca tcggcgcggg cgctcttgtg
     1261 ggccttgcgg tgttcgcccg gatcgatgcc gttggcgatc tgcttccggg cggcgtctcg
     1321 ccggttgcgg gcgtcgcgca gggacacatc aggatagaca cccatcgaaa gcgtcttgcg
     1381 cttactgtca aagcggtagt cgaagcgcca ccacttaccg ccgttggggt ggatcaacag
     1441 atagaggccg tcgccgtccg tcatcttgac gggcttgtcg gtgggcttcg cggacttgat
     1501 ggcggtgtcg gtaagtgcca tgacggtaac tccttggcgg taagtgcctg ttaccgtcta
     1561 agttaccgcc aaccgttacc ggcttgcaat aaacctcccc ggactaatgc ggacgtgaaa
     1621 aaggccgaaa cccttgattt ctcaggtgtt tccggccttc ttcggactac ttcggtatat
     1681 ggtttggtgg agctgggggg aatcgaaccc ccgtccgcaa gccctctaca gtcagttcta
     1741 catacttagc cgacctattt gaatttaacc cggggtttag ccagtcggca ggccgaccac
     1801 gggcgagtca cctaagattt cgcgcggggc caagtaaccc gaccctctgc tagttcctgt
     1861 gaatgacgct gcaatggagt tgcccccaat ccggcccagg aacgaaccgg tgcagcggct
     1921 taggccttaa gcggccagag cgaaacgctc gtcgttggcg tttgttgatt tccagtggat
     1981 ttacgaggtg actggtcctc ggtatgccct gatctgcttt gcaacccacg tcgaagccag
     2041 gtcagcccca cggtgatgaa acatcgattc tgcgggtgtg attctaccac gcagatcatg
     2101 gacatcagag gtgggcgaga agttcctccg ggtgtgagat gagggcatcg gcgccccacg
     2161 tgtcgatggc aggattggag cccagataac cgtaccgtac accgacagtt tgcatgccgg
     2221 ctgcccggcc tgctgtgatg tcgcggggat cgtcgccgac gtaaagactg cgggtcgggg
     2281 caacgcgcgc cagatcggcg gcatggagga ggggatcggg aaacggcttg cggcgggtca
     2341 gggtatcgcc accgacgacg caggcgcagc gtccgtccag gccgagggcg gtgatgatgg
     2401 ggcgggcgaa gcgttcggcc ttgttggtga cgacgcccca cgggagcccg cgctcatcca
     2461 gggcgtcgag cacaacttcc atgcctgtga agagacgggt gtggacgcac aggttcccgg
     2521 cgtagaggct caggaagcgg tcaaacaggg gggcgtattc cgcatggtcg ggcaaaattc
     2581 caagcccggc gctcagcatg ccgcgggttc cctgggagac aaaggggcgc agggactcga
     2641 agcgacaggg cggtagtccc aactcggcgc gcaactgatt gagcgcgacg gccagatccg
     2701 gcgccgtgtc agcaagggtg ccatccagat cgaagaagac cgcgtcagcc attgcgccgg
     2761 gtgcgcatca ggtaattgac gtccgtatcg cgaccgaggg cgtagatctg ggtgaagggg
     2821 ttgtagcgca tgccgatgag ctcttccacg tccagtccgg cgttccgtac atagcgggag
     2881 agctcggatg gcttgatgaa cttggcgtaa tcgtgagtgc ccttgggcag cagtttgagg
     2941 acgtactcgg cacccaccac ggccatcagg taggccttcg gattgcggtt gagggtggag
     3001 aagaagacgt ggccgccggg tttgaccagg gcggcgcagg cgcggatggt gctggccgga
     3061 tcggggacgt gttcaagcat ctccatgcag gtgacggcat cgaactggcc cggtgtctcg
     3121 cgggccatgt cttccgccga gatgtggcgg taatccacgg tctggccgga ttcatacagg
     3181 tgcaggcggg cgacacccag ggccttgtcg gagaggtcga ttccggtgac atgggcgccg
     3241 cgggcggcca tggattcaga caggatgccg ccgccgcagc ctacgtcaat gacctgtttg
     3301 ccggcgagat ggacggtctc gtcgatccag tcgagacgca gaggattgat ctggtgcagt
     3361 ggcttgaatt cggattccgg gtcccaccag cggtgggcaa gttcactgaa tttctgtagt
     3421 tcggcgggat cggcgttcat gatggaggct ggtcggaatc aggggcaatc agaaaccgga
     3481 attgtagtgc agccgttgcc gtgaggcggg cggacgaaaa acaaaaagcc ctgcagggca
     3541 gggctttttg aggacgtgcg ggagaagtga ttacttggtg ccgatcactt cgatttccac
     3601 gcgacgatcc ggctgcaggc actcgaccag cttcttgttc ttgccgttgt ccttgcccag
     3661 gcccttgcac ttgtcgccgg tcacgggctg cttcttgccc ttgccttcgg tgtagacacg
     3721 gttggccgca acacccttgg aaaccagata gtccttgacc gcggcggcgc gcttctcgga
     3781 cagcttctgg ttgtaggcgt caccaccgat gcggtcggta tggccgacgg cgaggatcac
     3841 ttcgaggttc agacccttgg ccttgtcagc caggtcatcc agcttggtct tgccagcggg
     3901 cagcaggacg gccttgtcga agtcgaacag ggtgtcggcg gccagcttga ccttttcgga
     3961 gaccggcgcg ggagcggcga tcggggcggc gcccttggca cccgcggcag gcgcggcagc
     4021 cttcggttcg cacttttcct tggccacgac atccttgtcg cattcacagc cgaccgggaa
     4081 ctcgccagcc atggtggtgg aagcggcggc gggggtccag tagccggtgc gccagcacag
     4141 atcggcgccg ctgcgggcaa cgacgttgcg gccatcgatc acgtagggca cttcaccctt
     4201 cgtatcgacg acgacatcct tcgcgaaact ggcggaagca gtcagaccga gggtcgccag
     4261 agcggcggcc atcagcagtt gttttttggc ttgtttgatc atcgttttcc tcgtcgcaag
     4321 gcaaaggggt tgaaccggag attcatccga tctgtccgac cgatgcaaaa tcaagtcagg
     4381 cagcatatcc aattcattgg cggagcctat tctgccatag ccgtgcaagg ctagacagtt
     4441 cagtgttgga tatttgcaac aaagtgccgt ctttgagcgc ctgccggcca gccacccaga
     4501 cgtctgaaac ggactcccgc ccgagcacgt agatcagatg cgaggcgggg tcgtagcagg
     4561 gcagcgtgtc gacgtcgtcc aggcgcacgg cacacaggtc ggcggccttc cccggttcga
     4621 tggagccgat gcggtcgtcc aggcccagcg cggcggcgcc gtcgagggtg gccatgcgca
     4681 ggacacggtg ggcgggcagc gcgccggcat cgcctgtcgt gagcttggcg aggagcgcgg
     4741 cctgatgcat ctcgcggaag aggtcgaggc ggttgttgct ggccgtgccg tcgctaccca
     4801 ggccgatccg cagtccgcgc tccgccactg ccgccatggg cgcggcaccg ctcgccagtt
     4861 tcatgttgga agtggggcag tgggcgatgg aacaatgatg cgtggccaga cggtcgatgt
     4921 cttccggtga cagatggacg gcgtggaccc cgatcaggcc agggccgagg agccccaggc
     4981 ggtccaggcg ggcaatcggc cgcaggccgt ggtcgcgcag gctgtcggcc acttcggtgg
     5041 cggtttcatg gagatggatg tggatgggca ggtccagctc ttcggccaat ccgccgacgc
     5101 gctcgaaggt tctgtcagag acggtgtacg gtgcgtgggg ggcgagggtg aaaccgatca
     5161 gggggtcgtg gcgccaggcg tcgcgggcgg cgaggccctt gcgcagatag tcgtcggcgt
     5221 cggcggcgta ctgggtggga aagtccagca cagtgatgcc cagcatggcg cgcaggccga
     5281 cgcggctgaa ggcttcggcg gcggcctccg gaaagaaata catgtcgttg caggtggtga
     5341 tgccgccccg caacatctcg gcggccgcga gcaggctgcc gtcgcgcacg aagcccggcg
     5401 agacgtgacg gccctccgcg ggccagatgg cttcctgcag ccagcgcatc agggggaggt
     5461 cgtcggcgag accgcgcatc agggtcatgg ccgcatggac gtgcagattg acgaggccgg
     5521 gtatcaggat gtggtctgtc agcgggcgga tctcgcgggg tcggaactgc cggcgggcgt
     5581 tgtcgatggg cagcaccgcg aggatgcggc cctcgtggat cgcaacggca tggcgttcga
     5641 gggtgaggcc cgcgggttgg accggaatga tccagcgggc ttcgatgagg aggtcgatgt
     5701 cggcaacggt ttcgacgggg gccatggagg gtccttgcag gcatgaaaaa ccccgcacaa
     5761 ggcggggtgg cgggggcggg ccttgcaggg tgcagattac ttggccggac ccttcacgtc
     5821 gatggtcacg cggcgattgg gcgccaggca gtcaacgagc ttcttgtggc ccagggaatc
     5881 gtcgcagccc ttgatgggct gggtcttgcc tgcgcccatg atctccacgt ccccggcctt
     5941 cagccctttg ccggcgagat agctgctgac ggctccggcg cgtttttcgg agagcttctg
     6001 attgtaagcc tgggtgccga ggcggtcggt gtggccggtg atgaggaggg cgcgcacgga
     6061 ggcgcactgg gcgctgcggg tcgccacctc atcgagggcc cgtcgggcgg cggggctgag
     6121 ggtggccttg ccaaaggcaa aggactggtc ggcgttaagg gtgacgctga aatcacaggt
     6181 ccggggggcg gctacggctg cggccggtgc ggcgaccgcc gccgcaggag cgccggacgc
     6241 gccgcgggcc gcgggcggct ggcatatgtc ggctccgacc acgtcccggt cacactggca
     6301 gccgacaggc agttcgccgg cctggacgct gccggcggcg gccggcgtcc agctgccggt
     6361 gcgccagcac aggtcggcgc cgctcttgac gacgacgttg cgctggtcga tggcgtaggg
     6421 tacgtcgccc ttcgggtcga cgacgatgtc cttcgactgg gcctgggcag tcaggccggt
     6481 gagggcgagc agggtggcgg acgccacgcg gaatgcggtg ttcatctggt ttctcctcct
     6541 gaaatggtcg gagccctcgg gggctcgtct tgtggggtgt gcgcggcgga ttagagcata
     6601 cgtccatcac cgatcacaat cgccgcccgg gtcgcgcctt ctcccggcag gatcgcatct
     6661 tccgcaggct tgcggacgcg ccggccggca cggtgcgcca tgctagaatt cacccttttc
     6721 gtcttttaga cggattgccg atccgctgcg gtgccatgat cccgttcgcc aaagaaaccc
     6781 tccccatcag tctcgaagac gagatgcgcc acgcctacct ggattacgcc atgagcgtga
     6841 tcgtcgggcg ggcgctgccc gacgtgcggg acggcctcaa gccggtccat cgccgcgtgc
     6901 tgtttgccat gcacgagctg ggcaatgact ggaacaaggc ctacaagaag tccgcccgta
     6961 tcgtcggtga cgtgatcggt aaatatcacc cccacggtga ccagtcggtg tacgacacca
     7021 tcgtgcgcat ggcccaggac ttttccctgc gctacatgct ggtggatggt cagggcaact
     7081 tcggttcggt ggatggcgat ggcgcggcgg ccatgcggta caccgaaatc cgcatggcca
     7141 agatcggtca cgagctgctc gctgacatcg acaaggaaac cgtcgacttc ggccccaact
     7201 acgacggctc cgaaaaagag ccgctggtgc tgccggcgcg catccccaac ctgctcatca
     7261 acggttccgg cggcatcgcg gtgggcatgg cgaccaacat cccgccccat aatctgggcg
     7321 aggtggtaga cgcctgtctg cgcctgctcc aggagccgga cgtctccatc gacgagctga
     7381 tcaagatcgt cccggcgccg gatttcccca ccgcggccct gatctacggc ctctccggcg
     7441 tgcacgaagg ctaccgcacc ggccgcggcc gggtggtgat gcgggcgcgc acccacttcg
     7501 aggacatcgg caagggcgac cgccaggcga tcatcgtcga cgaactgccc taccaggtta
     7561 acaagaaggc gctgctcgag aagatcgccg agctggtgaa cgacaagcgc atcgagggca
     7621 tcagcgagat ccgcgacgaa tccgacaagt cgggcatgcg cgtggtcatc gagctcaagc
     7681 gcaacgagat gccggacgtg gtgctgaaca acctgttcaa gcagacccag ctccaggaca
     7741 ccttcggcat gaacatggtg gccctggtgg acggcaagcc gcgtctgctt gacctgcgcc
     7801 agatgctgga atgcttcctc gcccaccggc gcgaggtgat cacccgccgc accgtcttcg
     7861 aactgcgcaa ggcgcgggag cgcggccaca ttctggaagg cctggcggtg gccctgtcca
     7921 acgtggacga gatcatcgcc ctcatcaagg ccgcgcccac gccgcccgaa gccaagaaat
     7981 ccctgatggc gcgccagtgg cgctcgcccc tggtggaaga gatgctggcc cgcgccgcct
     8041 ccgacagctt ccggccgact ggcctcgcgc cggaattcgg cctgtcggcg cagggatacc
     8101 ggctctccga cgcccaggcc caggcgatcc tggagctgcg cctgcagcgg ctcaccggcc
     8161 tggagcagga caagatcgtg gccgagtaca aggaagtgat ggagaccatc gccgacctgc
     8221 tcgacatcct cgcccggccc gaacgcatca ccgccatcat cggcgacgag ctggccctca
     8281 tcaaacagca gttcggtgat ccgcggcgct cggaagtggt gctcaacacc gccgacatca
     8341 atatagaaga cctcatcgcg ccccaggaca tggtcgtgac cctgtcccac acgggctact
     8401 tcaagcgcca gcccctcacc gactaccgcg cccagaagcg cggtggtcgc ggcaagcagg
     8461 cggcggcggt gaaggaagac gatttcgtcg accagctctt cgtggccaat acccacgacc
     8521 acatcctgtg cttctcgaac cgtggccggc tgtactggct caaggtgtac gaagtgccgg
     8581 agggcaaccg caacgcccgc ggcaagccca tcgtcaatct cttcccgctg ctcgaaggcg
     8641 agaagatcac cgcggtgctg ccggtcaagg aattcgacga aggccacttc gtgttcatgg
     8701 ccacggccca gggcacggtc aagaagacgc cgctcaccga cttctccaac ccgcgcaagg
     8761 ccggcatcat cgccgtggct ttggacgagg gcgactacct ggtcggcgtg gccatcaccg
     8821 acggccagca cgacgtgatg ctgttctccg acgccggcaa ggcagtgcgc ttcagcgaga
     8881 atgacgtgcg ccccatgggc cgcaccgccc gcggcgtgcg cggcatgatg ctggaggacg
     8941 accagtcggt catcgccctg ctggtggccg gcgacgaatc gcccagtgtg ctgaccgcta
     9001 ccgagaacgg ctacggcaag cgcacgccca ttgccgaata cacccgccat ggccgcggca
     9061 ccaagggcat gatcgcgatc cagacctccg accgcaacgg caaggtggtg gcggccacgc
     9121 tggtcaccga gaaggagcag atcatgctca tcaccaccgc cggcgtgctg gtgcgcaccc
     9181 gcgtctccga gatccgcgag atgggccgtg ccacccaggg cgtgacgctg atctctgtgg
     9241 atgagggcag caccctgtcc ggcgtgcaga agatcgtcga aagcgaagag gacgagcagg
     9301 acggcgaggg cccgcgggcc gacgcctgat tcaccgcaag caaggaagca gacgcgatga
     9361 ggatctacaa cttctcggcc ggtccggccg cattgcccac cccggtactc gagcaggtgc
     9421 gcgaggagct cctcgactgg cacggcgccg gcatgggcat catggaaatg agccaccggg
     9481 gcaaggactt catgtccgtc atcgccgccg ccgaggccga tctgcgcgcc ctgatgggca
     9541 tcccggacgg ctacaaggtg ctgttcctgc agggtggcgc cacccagatg ttcgcgcaga
     9601 ttcccatgaa cctgctggcc gggcgctccg ccgattacgt ggtgaccggc gcctggtcca
     9661 agaaggcctg gaaggaagcg cctcacatcg gccccgtgcg tctggctggc acgaccgaag
     9721 ccgacggccg cttcacccgt ctgccggcag cggatgaact gagcttcgat ccggacgccg
     9781 cctacgtgca cctgtgcacc aacgagacca tccacggcgt tgaaatgttt gatctgcctg
     9841 ccacgcccgc cggcgtgccg ctggtggcgg acatgagctc ccacatcctg tcgcgcccga
     9901 tggacgtgtc ccgctatggc ctgatctatg ccggagccca gaagaacatc ggcccggccg
     9961 ggctggtgct ggtcatcgtg cgcgacgatc tcctcggccg cgcctccgcc gccacgccca
    10021 gcgtgatgaa cttcgccacc atggccgagc atggttccat gctcaacaca ccgcccacct
    10081 tcggcatcta tctggctggg ctgatcttca aggatctgct ccgccagggc ggactggcgg
    10141 ccgtcgaggc ggccaaccgg gccaaggcag agcggctcta cgacgccatc gacggcagcg
    10201 gcggcttcta ccgcaacccg gtggatcgtc cctgccggtc cctgatgaac gtgcctttca
    10261 cgctggccga tccggccctg gaggcggatt tcctcgccga ggcggcggcc cgccggctgg
    10321 ccgggctcaa ggggcacaag tcggtgggcg gcatgcgcgc gtcgatctac aacgccatga
    10381 cccttgacgg tgtcgaggcg ctggtggatc tgatgcagga tttcgcacgg cgcaaggcgt
    10441 gagcgccgga ctttcgggag caggcatgaa cgaggcgatg ctgcaggaac tcggtgcggt
    10501 ccgcgaccag atcgatgcac tggacgccca gatgctggcg ctgctcaacg cgcgcgccag
    10561 atgtgcccag cacgtgggcg agatcaaggc ccgccacggt gaggccgggc aggtctaccg
    10621 gccggagcgg gaagcccagg tgctgcgccg catccaggcc ctcaacgccg gtccgctgcc
    10681 ggacgagagc gtcaccttct tcttccgcga ggtgatgtcc gcctgcctgt ccctcgaaaa
    10741 tccgttgggc gttgcctttc tcgggccgct gggcaccttt tccgagagcg ccgcggtcaa
    10801 gcactttggc cacgccgcgc ggctggcgcc ccagaccacc atcgacgacg tgttccgtga
    10861 agtggagtcc ggtcacgccc agtatggcgt ggcgccggtg gagaattcca ccgagggcgc
    10921 cgtcggtcgc acgctcgacc tgctgctgtc ctcgcccctg cagatctgcg gcgaggtcgt
    10981 gctgcgcatc catcagcacc tgatgagcgg tgagactgcg ctgggcgacg tccgcaaggt
    11041 gtattcccac gcccagtccc tggcccagtg tcacgaatgg ctcaacagcc atctgcccgg
    11101 cgtgccgcgc atttccgtgg ccagcaacgc cgaggcggcc cgcctggcgg cctccgagcc
    11161 cggcgcggcg gcgatcgccg gtgaggccgc ggcggagcgc tacgcgctgc cgaccctcgc
    11221 ccgcagcatc gaggacgagc ccaacaacac cacccgtttc ctggtgctgg gccgtcatga
    11281 cgccggtccc agcggcgccg acaagacctc tctgatcatg tctgcagcca accgcccggg
    11341 cgccatgcac gcgctgctgg cgcccctggc ggacgccggg gtgtccatga cccggctcga
    11401 atcccgcccg gcgcgggcgg ccctgtggga atacgtgttc tacgtggact tcgccgggca
    11461 tcgcaatgat ccggaggtcc gcaaggccct ggacgccctg agcgcccacg cctctttcct
    11521 gaagatcctg gggtcctatc cccaggctgt gtactgaacc cccggagcct tccatgcgtc
    11581 tgtgcgatct gtcgccggcc cacatccgcg cgatttcacc ctatcagccg ggcaagccca
    11641 ttgccgagct ggcccgtgag atggggctgg cggagtcgtc catcgtcaag ctggcgtcca
    11701 acgagaatcc cctcggcttc ggcgacaagg cgcgggcggc gatggccgca gccctcgacg
    11761 atctggcgcg ctacccggac ggcaacggct tcgagctgaa acaggccctg tgccgtcgct
    11821 acggcgtggc gatggatgag atcgtcctcg gcaacggctc caacgacgtc ctggagcttg
    11881 ccgcgcgggc ctttctaggt ccgcagacct cggccatcta cgcccagcac gcgtttgccg
    11941 tgtatcccct ggctacccag gccgtcggtg cgcggggcgt cgaggtgccg gccaggcact
    12001 ttggccacga tctggacgcc atgctcgcgg cgatcacgcc ggacacccgg gtgctcttca
    12061 tcgccaaccc caacaatccc accggcacct tcgtgcccgg cccggaactg gaagccttcc
    12121 tcgtccgcgt gcccgaccac gtgctggtgg tcctcgacga ggcctacacc gaatacctcg
    12181 gcgaggaaca gcgctacgac gccattgcct ggctggcccg cttccccaac ctgctggtgt
    12241 gccgcacctt ctccaaggcc tacgggctgg ccggcctgcg ggtgggctac ggcctgggcc
    12301 atccggcggt catcgacctg ctcaaccgcg tgcgccagcc cttcaacgtg ggcagcgtgg
    12361 ccctggcggc agccgctgcg gcacttttcg acagcgattt cctggcacgc agctatgaag
    12421 tgaaccgtgc cggcatggcc cagctcaccg ccggcttcgc ccgcctgggg ctggagtgga
    12481 ttccgtcggc aggcaatttc gtcacgttca gggcaggcga cggtgccggt gtcaatcggc
    12541 gcctgctgcg ccagggcgtc atcgtgcgcc ccatcgccgg ctacggcatg cccgaatggc
    12601 tgcgcgtctc gatcggcctg gaagccgaga acgcgcgctt cctcgaagcg ctgccccagg
    12661 cactgggctg aggacggcgc ggtgcgcaag atcgacaagc tggtcatctg cggcgtcggc
    12721 ctgatcggcg gatcggtggc cctggccctg cgccaggcgc gcctggtgaa tcgcatcgtc
    12781 ggcgtcggcc gccgccgcga aaccctggaa caggccctgc agctgggcgt catcgacgag
    12841 atctccaccg actgggccga ggccctcgat ggcgccgaga tcgtcctgct ggccatgccg
    12901 gtggggcaga ccgacgcggt ggccgccgcc atggcgcccc atctggggcc ggacaccctg
    12961 gtcaccgacg ctggcagcac caagcgtgat gtcatcgagg ccatctatcg ccacctggcc
    13021 gcccacctgc cccgcgtggt gccggcccat cccatcgcgg gagccgaaaa gagcggcccg
    13081 tcggcggcgc gggcggacct gtaccgcaag cgcaaggtgg tggtcacgcc cctgccagag
    13141 aacgatgccg tggccgtcgc gcgggtgcgc gaactgtggg cagcctgcgg tgcgcacctc
    13201 ttcgagatga cgcctcagga gcacgaccgg gtgttcgccg cggtgagcca tctgccccat
    13261 ctgctggcct tcgggctggt gcatgacctg gccgggcgtg ccaacgcgga ggagctcttc
    13321 agctacgccg cgagcgggtt ccgcgatttc acccgggtgg ccggcagtca tccggaaatg
    13381 tggcgcgaca tctgtctcgc caatcgtcat tccctgctcg ccgagctgga cgcttacctg
    13441 ggcgaactcg cctggctgcg ggcgctgctc gcctcgggcg atggtccggc cctggaaaag
    13501 gttttctccg aggccagccg ggcccgcaac gaatgggccg acaaggcttt tcccgcgacg
    13561 ccttcgggcg agtaattcaa ggatctttca tggaattcat cgatttgccg ccgatgctca
    13621 gggccgtcgg cgaggtgcgc ctgcccggct ccaagagcat ctccaaccgc gtgctgctgc
    13681 tggcggcgct ggcggaaggc acgacgcgtg tcgaggcgct gctggattcg gacgacaccc
    13741 gcgtcatgcg cgacgccctc gtggcgctgg gtgttggcct ccaacaggag ggtgacacgc
    13801 tggtggtgac gggttgcggc gggcgtttcc cgaacgcgcg ggccgacctc ttcgtgggca
    13861 actccgggct cagcatccgc accctggtgc cggccgtcgt ggccagcctg gcgggctgcg
    13921 gggatgcgga tgcggcggtg cgcctgtccg gcgtgccgcg catgcacgaa cggcccatcg
    13981 gcgatctggt ggacggcctg gctcagctcg gtgcccgcgt cgattggctc ggccagccgg
    14041 ggtttccgcc cctggcgctg cgtccggcgc ccctcgccgc ggagacggtg agcgtgcgcg
    14101 gcaacgtctc cagccagttc ctcaccggcc tgctccaggc tgcgccgctg gtggcccgcg
    14161 acacgcccct cgtgatcgtc gtggaaggcg agttgatcag ccggccctac gtggaaatca
    14221 ccctcaatct gatggcgcgc ttcggggtgc aggtggagcg ggacggctgg agccgcttcg
    14281 tcgtgccgac cggccagcac tacgtgtcgc caggacggat cgtggtggaa ggggatgcct
    14341 ccacggccag ctacttcctt gccgcaggcg ctctgggggg cggcccgctg cgggtgaaag
    14401 gggccggacg cggcagcatt cagggtgacg tgcgctttgc agatgcgctc gccgaaatgg
    14461 gcgcgcagat tacctggggc gaggactgga tcgaggcgcg ggcgccggag tcagggcgac
    14521 tcaagggcat cgacctggat ctcaatcaca ttccggatgc cgccatgacc ctggccgtgg
    14581 cggccctcgt cgccgacggc ccgagccatc tgcgcaacat cggcagctgg cgcgtcaagg
    14641 agaccgaccg catcgccgcc atggccaccg aactgcgcaa gctcggggcg accgtcgagg
    14701 agttccccga gagtctgcgg atcacgccgc ccgcgcgcct gcagcctgcg gtgatcgata
    14761 cctacgaaga tcaccgcgtt gccatgtgct tctctttggc gaccctgtgc ggcaccagcc
    14821 tgcgcatcaa cgatccgaag tgcgtcaaca agaccttccc cggctatttc gagaccttcg
    14881 ccggcatcac cgcgccggtg ccggtggtgg ccatcgacgg accgtcggca tccggcaagg
    14941 gcaccgtggc tgcgcgggtt gcccaggctc tgggctttgc gcacctggac agcggatccc
    15001 tgtaccgcat tgtcgccctg acggcgatcc gccgcggtgt ggccctcgac gacgagccga
    15061 cgctggccgc catcgccgcc gatctgccgg cccgcttcga gggcgatcgt gtgatgctgg
    15121 agggcgacga cgtcaccgag gcgatccgca gcgaagcctg ctcggtgggg gcgtcccgcg
    15181 tggcggccct gccgaccgtg cgtgaggccc tgttctggcg tcagcgcgcc tatcgccgtg
    15241 cccccggtct ggtgggcgaa gggcgcgaca tgggctcggt ggtctttccc gatgcgcccg
    15301 tgaagatctt cctcaccgct tccgtcgagg cgcgtgcgga aaggcgctat aagcagttga
    15361 tggataaagg aatgcctgct aacatggccg atcttctcaa ggatttgcgt gaacgcgacg
    15421 cccgggatgc ccagcgcgcg gccgcaccct tgaagcagga agaggatgcg gtgctgctgg
    15481 acacgtccga tatgtctgtg gaacaggctg tggcgtttgt gctggagcgg gccgcgatcc
    15541 tgaagggctg atcccttttt ttgacgcgtt ctccccggtc tgcccggtcc ggggaggttt
    15601 ttcaaccagg agccgtctcg cgacggttgt agggtatttt ttcaatatgt ccattgccac
    15661 ccctgtttcc atcgaggaaa gttttgccgc cctgttcgag gaaagcctca atcgtcagga
    15721 gatgcgtgcc ggtgaagtga tcaccgccga agtcgtgcgg atcgaccaga acttcgtcgt
    15781 cgtcaacgcc ggcctgaagt ccgagagcta catccccctc gacgaattcc gcaacgaccg
    15841 cggcgaactg accgccaacg tgggcgatta cgtccaggtt gccatcgagc aactcgaaga
    15901 cggttacggc gaaacccgcc tgtcccgcga caaggccaag cgcatcgccg cctggaacga
    15961 cctcgacaag gccctcaacg aaggcattct ggtcaagggt gtgatcaccg gcaaggtcaa
    16021 gggcggcctg acggtgatgg tcaatggcat ccgcgccttc ctgcccggct ccctggtgga
    16081 catgcgtccg gtcaaggaca ccaccccgtt cgagggcaag gaattcgaat tcaaggtcat
    16141 caagctcgac cgcaagcgca acaacgtggt cgtgtcccgc cgtgccgtgc tggaagcatc
    16201 catgggcgaa gagcgtcaga agctgctcga aaccctcaag gaaggcaccg tcgtcaaggg
    16261 cgtggtcaag aacatcaccg actacggcgc gttcgtggat ctgggcggca tcgacggcct
    16321 gctgcacatc accgacctgg cctggcgccg cgtccgtcac ccctccgaag tgctgtccgt
    16381 gggtgacgaa ctcgaagcca aggtgctcaa gttcgaccag gagaagaacc gtgtctccct
    16441 gggtctcaag cagctcggcg aagatccgtg ggtcggcatc tcccgccgtt acccgcaggg
    16501 cacccgcctg ttcggcaagg tcaccaacat caccgattac ggtgcgttcg tggaagtcga
    16561 gcagggcatc gaaggcctgg tgcacgtctc cgaaatggac tggaccaaca agaacatcca
    16621 cccgaccaag gttgtccagc tgggcgacga ggtcgaagtg atgatcctcg agatcgacga
    16681 agaccgccgc cgcatctccc tgggcatgaa gcagtgcatg tccaacccgt gggacgattt
    16741 cgccatcaat cacaagaagg gcgacaaggt ccgcggccag atcaagtccc tgaccgactt
    16801 cggcgtgttc atcggcctgg aaggcggcat cgacggtctg gtgcacctgt ccgacctgtc
    16861 ctggtccctg gcgggcgaag aagccgtgcg ccagtacaag aagggcgacg aagtggaagc
    16921 cgtggtgctg aacatcgacg tcgagaagga gcgcatctcc ctcggcatca agcagctcga
    16981 aggcgacccc ttcaccaact tcatcgccac ccacgacaaa aacaccctcg tgaccggtac
    17041 cgtcaagtcc gtggatgccc gcggtgcggt ggtggccctg aacgatgacg tggaaggcta
    17101 cctgcgtgct tccgagttct cccgtgaccg cgtggacgat ctgtcccagc tgctcaaggt
    17161 gggtgacgcc gtcgaggccc tgatcgtcaa cgtcgatcgc aagacccgcg gcatcaacct
    17221 gtccgtccgt gcgaaggatc aggtcgaaca ggccgaagcc atgaacaagc tcgcttccga
    17281 cagcggctcg gctgccagcg gcaccaccaa cctgggtgcg ctgctcaagg ccaagctcaa
    17341 cgaacagaag taagcggcac cggcatgacc aagtcggagc tcatcgcccg gctggcggag
    17401 cgttttcccc agttggtggc gaaggacgcc gaaaccgctg tcaagatgat cctcgatgcg
    17461 atgaccgacg ccctctcgcg gggtgatcgc atcgagatcc gcggctttgg cagctttgcc
    17521 ttgaactatc ggccgccccg cgttggacgc aatccgaagt cgggggagaa ggtgcaggta
    17581 cctgaaaagt acgtgcccca tttcaaggcc ggcaaggagc tgcgtgagcg ggtggacatg
    17641 attgtctgat cgccgcggcg tgccgcggtc tggttcggcc catactggaa ggcggcgatt
    17701 gccgccttct tttttttggt cccgtcgggg ataatttcgt catgcgcctg ctcacctgga
    17761 tcgtccgcct gttcctgttc ctcgtcctct tcggctttgc cgtcaagaac gacgatgtag
    17821 tcaccctgcg gtttttcttc ggtgcccagt ggcaggtgcc gctggtgctg gtgatcctac
    17881 tctttgtcgt gatcggggtg ctgatcggag tcaccgccgc ggtcgcgacc ctgtaccgcc
    17941 agcatcggga gcttgggcaa ctgcgtgagc gtcaggccga tgcgccgccc cccgtgactg
    18001 tccgcccgcc cgtggcccac ggccccgatc tgcccgagac ctactgatcc cccatggaat
    18061 tcgaactctg gtggctgctg gcgtttccgc tgttcttcgc acttggctgg ctggccgcgc
    18121 ggatcgacat caagcatctg gtcaaggaat cccgcgcctt gccgcggacc tacctgaccg
    18181 gtctcaattt cctcctcaac gagcagcccg acaaggccat cgatgccttc atcgaggcgg
    18241 tcaagatcga caatcagacc gttgagctgc atttcgccct gggtagcctg ttccgccggc
    18301 gcggcgagac cgaccgggcg atccgcatgc accagaacct catcgaccgg gacgacctgt
    18361 cccaggatca gcgcctgcag gccctgtcgg agctggggct cgactacctg aaggccggtc
    18421 tgctcgaccg ggccgaggcg gtcttcgttc agctgcgggg ctcccggctc gacgaattcg
    18481 ccctcaagaa tctcctggac atctaccagc aggaaaagga ctggcgcaag gccatcgaca
    18541 tcgcccaggc gctgccgggc catgagagcg ccctgtggga aaaagaggtc gccaacttcc
    18601 actgtgaact ggcggcagcg gccatcgccg attcgcgctt tgatgaggcg cgttcgaatc
    18661 tggagcgggc ccttgagacc aatcgcaagt cggtgcgcgc gagcatcctg ctgggtgatc
    18721 ttcatgccgc ccagggtgac gacgaagcag cgctggaggc ctggaagcgt gtcgagaccc
    18781 agaatccggt ctatctggca ctggtggccg aacgcatcat ggacgcctat cggcgcctcg
    18841 gccgactgga gcagggcatg cagctcctgc gcagctacct ggacagctac gcctcgctgg
    18901 atctgctgga tgcggtcttc cagtgggaac tccagaagga gggggcccag gcggcgtatc
    18961 acctggtccg cgaggagttg cgccgcaatc ccaccctgct gggcctcgac aaattgctcg
    19021 aagcggcctt gctcaccgca ccggtcgaac agcgcaccga tatcgaactg gtcaagggtc
    19081 tggttcatgg ccatacccgg cgggtggcgc gttatcgctg cgatgcctgc ggtttcaagg
    19141 cacggcagtt ctactggcgc tgcccggcct gcggcggttg ggaaacctat ccgccgcggc
    19201 gcactgagga attcgatctg acgccctgag gcgcgccttg cacaaggatg tctgcggccg
    19261 gatcggaagt gggtggcatg aacccgacaa tttttgtcgc tggggtcagg ccggacggac
    19321 gaatcccgcg cagtgggcgc gtttgtccgg ctggcgcggc gcatgcttgc gatgatcggg
    19381 ggcgccatcc ggtatgatcc gccccaatcc ccgcatccac cggactgtca catggaattc
    19441 aagacactcg aggactacgt gggccagacg cccctggttc gcctcaaacg catcaccgct
    19501 gggcgcaaca atacgatcct ggccaagctg gaaggcaata atcctgccgg ctcggtcaag
    19561 gatcggcccg cgctgtccat gatccttcac gccgaagcgc ggggcgacat ccggcccgga
    19621 cagacgctca tcgaagccac cagcggcaac acgggcatcg ccctggccat ggcggcagcc
    19681 atgcgcggct acggcatgat cctggtgatg cccgagaacc agagcatcga gcggcgccag
    19741 accatgcggg ccttcggcgc cgagctggtg ctcacgccca aggagggcgg catggaactg
    19801 gcgcgggacg tggccgagcg catgcgcgac gaaggtcgcg gcgtgatcct cgaccagttt
    19861 gccaacccgg acaatccgct ggcccacatc gaaggtaccg ggcccgagat ctgggatcag
    19921 accggcggca cggtcaccca ttttgtgagc agcatgggca ccaccggcac catcatgggc
    19981 gtatccacct acctcaaggc gcgcaacccg gcgatccgca tcattggctg ccagcctgcc
    20041 gacggggccc agattcccgg catccgcaag tggcccgagg cctatcttcc gcgcatctac
    20101 gacaaggcaa gggtggatca gcttgagtac gtggcccagg cagacgccga ggagatgacc
    20161 cgccgcctgg cgcgggagga aggcatcttc gccggcatct cgtcaggcgg tgcgttgcac
    20221 gtggccctgc gcctcgccgc ccagctggag gacgcggtga tcgtgtccat cgtctgtgac
    20281 cggggtgacc gctacctctc caccggcgtg tttccggcct gagcgttcat ggttccgatc
    20341 ctggtcttcg acatcgagac gatcccggat gtggccggca tccgcgcgct gaacggcctg
    20401 ccggcagagc tgggcgacga cgaggtggcc gagttcgcct ttcagcagcg ccgtgctgcc
    20461 accggcaacg attttctgcc ccatcacctg cagcgtgtcg tgacaatctc gtgcgtgctg
    20521 cgggacgaca gccagttccg cgttttttcc ctgtcggaac cggacgcgac ggaagggcag
    20581 atcatccagc gcttcttcga cggcgtcgag aaattcaccc cccagcttgt atcctggaat
    20641 ggcggcggct tcgatctgcc ggtgctgcac taccggggca tgatgcatgg tgttgcggca
    20701 tccaggtact gggatctcgg ggatggcgac tatttcgatt cccgcgactt caagtggaac
    20761 aactacatcg ggcgctacca cacccgccac ctggatctga tggacctgct ggccctctat
    20821 cagccgcggg ccagcgctcc gctggatgat ctggcgaagc tgatgggttt tcccggtaag
    20881 ctgggcatgg atggctcagc cgtctggcag ggctaccggg atgggcgtat cgccgagatc
    20941 cgggattact gcgaaacgga cgtggtgaat acctatcttg tgctgctgcg cttccagcgc
    21001 atgcgcggga tcctcagcgc cgagcgctat gaagaagaga tcgccttcgt gcgcaagagc
    21061 ctgtcgagcc tgcccgcacg tcactggcag cgttttctgg aagcctgggg cgatggaagt
    21121 tcataatccg atagtccaaa ataatcttga cttccttctc tcagccttta taatgcacag
    21181 cttcttaggc gcgtagctca gttggttaga gcaccacctt gacatggtgg gggtcgttgg
    21241 ttcgagtcca atcgcgccta ccaaacacct gagaaggaaa acggatgttc cgaacttgca
    21301 gccgctccga ttgatgcatt cgagtccgct gcaaggcgtt ttccagtcat gaaaaaagtg
    21361 cggctcgtcc gcactttttt tttgccctga gccagtcatc atgcccaaca tcaccctgcc
    21421 cgacggttct gtacgcagct tcgatcagcc cgtgacgatc gcccaggtcg ccgcctccat
    21481 cggcgccggc cttgcccgcg cggccctggc cggcaaggtc gacgggcgtc tcgtggatac
    21541 ctccttcctg atcgaccggg acgtgcagct cgccatcgtc accgacaagg acgcagacgg
    21601 gctggagctc atccgtcact ccaccgccca cctgctggcc tatgccgtga aggcgctgtt
    21661 tcccgacgcg caggtgacca tcggaccggt gatcgagaac ggcttctatt acgacttctc
    21721 ctatacccgc gcgttcacgc ctgaggatct ggcgaagatc gaggcgcgca tgctggaact
    21781 ggcaaagaag gacattcccg tcgaacgcag cgtgttgccg cgggacgagg cggtggccta
    21841 cttcaagtcc atcggcgagc actacaaggc cgagatcatc gcttccatcc ccgccaatga
    21901 ggatgtttcc ctgtacaagg aaggcgactt caccgacctg tgccgcggcc cccacgtgcc
    21961 ttcgacgggc aagctcaagg tcttcaagct gatgaaagtg gccggcgcgt actggcgcgg
    22021 cgactctcgc aacgagatgc tgcagcgcat ctacggcacc gcgtgggcaa agaaggaaga
    22081 tcaggacgcc tacctgcaca tgctggagga ggccgaaaag cgtgaccacc ggcggcttgg
    22141 ccgccagctc gatctcttcc atcttcagga tgaggcgccg ggcatggtgt tctggcatcc
    22201 ccacggctgg accctgtggc aacaggtcga gcagtacatg cgcagcgtcc tcaatgaggc
    22261 gggctaccag gaggtgaaga cgccgctggt gatggatcgc gccctgtggg agaaatccgg
    22321 tcactgggac aactatcgcg acaacatgtt cacgaccgag tcggagaagc gcgactacgc
    22381 gatcaagccg atgaactgcc ccggtcacgt gcagatctac aaccacggcc tgcattccta
    22441 ccgggacttg ccgctgcgcc tcgcggaatt cggctcctgc caccgcaacg aaccgtccgg
    22501 cgcgttgcat ggcctgatgc gggtgcgcgg cttcgtccag gacgatgccc acatcttctg
    22561 taccgaagag cagatcgtgt ccgaggtgac ggccttcaac gaactgctga cccgcgtgta
    22621 cagggatttc ggcttcaccg atgtggcggt caagctctcc ctgcgccccg agaagcgggc
    22681 cgggaccgac gccatctggg acaaggcgga aaccggtctg cgtcaggcat tggctgcgtc
    22741 cgggcattcc tgggaggaac tgccgggtga gggcgcgttc tacggtccga agatcgaata
    22801 tcacatcaag gacgccctcg gccggtcctg gcagtgcggc acgatgcagc tcgatttcgt
    22861 gctgccagag cgccttggcg ccgaatatgt ggccgaggac aacgcccgat atcgtcccgt
    22921 gatgctgcac cgggccattc tgggttccct ggagcgtttc atcggtatcc tcatcgaaaa
    22981 ccatgccggc gcgttgccgc tgtggctctc tccggtgcag gctgtggtga tgaatatctc
    23041 cgaagcccag gccgattacg tgacggaagt ggtgaatcgc ttgcgttcac gcggcttccg
    23101 cgttgaagcg gatttgcgga acgagaaaat tacttataaa atacgcgaac atagcgtacg
    23161 aaagctgcct tacctgctgg tcatcggcga taaagaaaag gcagcagatg tcgtcgcagt
    23221 gcgcgcccgt ggcaatcaag acctcggtca aatgtccctc gaacaggttg tcgagcgttg
    23281 gcagcgtgag cttctcgcgc gagccggcac ggcgtgattt tttgtttttc aaggaggctt
    23341 gaaccatcgc tcaggaaaag aagctgcgca tcaacggcga aatcactgcg ccggaagtgc
    23401 gtctgacggg cccggacggc gaacagatcg gaatcgtgtc cctgcgtgcg gcactggaag
    23461 ccgcggatga agccggtgtg gatctcgtcg agatcgcacc gatggccaag ccgccggtat
    23521 gccgtctgat ggacttcggc aagtacaagt acgaagaagc caagcgcgcg cacgaggcca
    23581 agcagaagca gaagcagatc caggtcaagg aagtcaaatt gcgtcccggt acggacgaaa
    23641 acgactacca gatcaagctg cgcaacctca tcaagttcct gaacgaaggc gacaaggcca
    23701 aggtgacgct gcggttccgc gggcgcgaaa tggcgcacca ggaattcggc atgcgtcagc
    23761 tggagcgcat caaggccgac ctggaggaaa tcgcccaggt ggagcagatg cccaagatgg
    23821 aagggcgcca gatgatcatg atcatcgcgc ccaagcgcaa gcactgagcg cgtattgaat
    23881 tcccggtcct gcgaggggcc ggaaatccgg ccggcgaaaa aagccagccg ggcacaagtg
    23941 gtgacgggtt gttgaagcgt gtccggaagt cggatgccac ccggggccat gacataaaag
    24001 gagtccttca tgcccaagat gaagaccaag agcggagccg ccaagcggtt caaggtccgc
    24061 tcgagcggca gcatcaagcg ttcccaggcg ttcaagcgcc acatcctgac caagaagacc
    24121 accaagagca agcgccacct gcgcggcatg acctgcgtgc atgcctcgga cgaaaagtcc
    24181 atccgcgcaa tgatgcctta cgcctgaatt aggagaacgc catgccccga gtcaaacgcg
    24241 gtgtaaccgc ccgtgcccgt cacaagaagg tcctcgatca agccaagggc tatcgcggcc
    24301 gtcgcaagaa cgtctatcgc atcgccaaac aggcggtgat gaaggccggt cagtacgctt
    24361 atcgtgaccg tcgtcagcgc aagcgtcagt tccgcgccct gtggatcgcg cgtatcaacg
    24421 ccgcgtcccg cgagctcggc ctgacctaca gccgcttcat gaatggcctg aagaaggccg
    24481 cgatcgaagt ggaccgcaag gtgctcgccg atctggccgt attcgacaag cccgcgtttg
    24541 cggcgctcgc cgagcaagcc aaggccaaac tcgctgcgtg atccaacgca gcataaaaaa
    24601 aggaggcctg gcctcctttt ttttatcctg aacccggcat tcgactgaaa aatggatcaa
    24661 ctcgatcaac tcgtcgcgca ggcaggcgcc gatttcgccg ccgccgccga cagtgccgcg
    24721 ctggaaaccg ccaaggcgcg ttttctgggc aagggcggct ccatcaccga actcctcaag
    24781 ggcctcggca agctcgacgc ggaggcgcgc aaggccgccg gcgctgagat caacaaggcc
    24841 aagtccgcca tcgaagcggc cctggaggcc cgccgtgaag ccctgcgcca ggcggcgctg
    24901 gaagctcagc tggcggccga agcactggac gtgaccttgc ctggccgggg cgccggtctt
    24961 ggcggtctgc acccggtggt gcgcaccctt gagcgtgtcg agcagctgtt ccgctccatt
    25021 ggcttcgaag tggccgacgg cccggagatc gagaccgatt tctacaactt cactgcgctg
    25081 aacacgccgg ccaaccaccc ggcccgttcc atgcacgaca ccttctacct ggaaaacggg
    25141 gaaggcgtgc tcctgcgcac ccacaccagc ccggtgcaga tccgtgccat gcaggcccat
    25201 gtcgatgcct acaaggaccg cgacggcatg cccgaattgc gcgtcatcgc gcccggccgc
    25261 gtgtatcggg tggattctga cgccacccat tcgcccatgt tccatcaggt ggaaggcctg
    25321 tgggtcggcg aatcggtgag ttttgccgac ctgaagggcg taatcgccga cttcctgcgc
    25381 cgcttcttcg agagcgacga cctgcaggtg cgcttccggc cgtccttctt ccccttcacc
    25441 gagccgtccg ccgagatcga cgtggccttc atgagcggcg cgctcaaggg ccgctggctg
    25501 gaaatcgccg gctgcggcat ggtccatccc aatgtgctgc gattcggcgg catcgatccg
    25561 gagcgctaca ccggctttgc cttcggcatg gggcccgacc ggctcaccat gctgcgctac
    25621 ggcgtcaatg atctgcgcct gttcttcgaa ggcgatctac gtttcctcgg tcaattcagg
    25681 taagccatgc aattctcaga actctggctg cgcagtttcg tcagcccggc cctcgacgcc
    25741 gacgccctcg gccatctgct caccatggcg gggcttgaag tggaagagca gggcggtgtg
    25801 gccgcggcct tctccaaggt ggtggtggcc cgcatcctcg aagccgacaa gcatcccaac
    25861 gccgacaagc tgcgcgtctg caaggtggat gccggtcagg gcgaaccgct gcagatcgtc
    25921 tgcggcgccc ctaacgcggc ggccggcctc acgattccct gtgccctggt gggcgccgaa
    25981 ctgcctggct tcgccatcaa ggcggccaag ctgcgcggcg tcgaatcctt cggcatgctg
    26041 tgctcggcca aggagctggg catcgccgac gacagcagcg gcctgctggt gctgccggag
    26101 gatgcgccgg tgggcaccga cctgcgcgac tatctggccc ttgacgacac cgtctacacc
    26161 atcaagctca cgcccaaccg ggccgactgc ctgagcctga ccggcatcgc ccgggaagtg
    26221 gcggcgctga ccgatcgtcc cctcagtctc gtggacacgt cctccgtggc gcccaccatc
    26281 gcggacaccc gcaaggtcgt gctcgacgcc cccgaggcct gcccgcgcta ctgcggccgc
    26341 gtcgtccgcg gcgtgaacgc ccaggcgcca acgccggatt ggatgaagca gcgcctcgcc
    26401 cgcagcggca tccgttccat cagcgtgctg gtggatgtga ccaactacgt catgctggag
    26461 ctgggtcagc cgctgcatgc cttcgacaac gccaggctcg acggcgccat ccacgtacgc
    26521 atgcccaagg cgggcgagca ggtgctgctc ctcaacggcc agaccgtcac gccggcagcc
    26581 gacaccctgc tgatcgctga tgagtctcgt gccctggccc tggccggcat catgggcggt
    26641 gaggagagcg gcatcaccct ggaaaccacc gacctgttcc tggaaagtgc cttctttgcg
    26701 cccgacgcca tcgccggccg ggcgcgtgcc tatggcttct cgtcagatgc ctcctaccgt
    26761 ttcgagcggg gcgtggactt cgccctggcg ccggccgccc tggagcgtgc cacgcgcctg
    26821 atcctcgacc tgtgcggcgg cgaagccggc ccggtggtgg aagccctggc agccgaccga
    26881 ctcccgcagc gggcgccgat ccgcctgcgc gcggcccgcg tgcgccgggt gctgggcatc
    26941 gacgtctcgg cggacgaggt ggcagatctg ttcggccgcc tcaatctggc cgtgaatcgc
    27001 gatggcgacg acttcgtcgt cacgccgccg tcctaccgct tcgacctgga gatcgaggaa
    27061 gacctcatcg aggaaatcgc ccgtctgcac ggttacgaca acattcccac cgtcgtgccc
    27121 aagggcccca tcgccatgct gccgcgcccc gaggcgcgcc gcgatccgat gggcgtccgc
    27181 cgcctcgtgg ccgcgcggga ataccaggaa gtggtcaatt tttcctttgt ggaagaggcc
    27241 tgggagcggg atttctgtgg caatgccgac ccggtgcgcc tcgccaaccc catcgccagc
    27301 cagatgagcg tgatgcgctc cagcctgatc ggcggtctgg tggccaacgt ggtcaccaac
    27361 cgcaagcgtc agaacaatcg ggtgcgcgtc ttcgagctcg gtcgcgtatt ccgtcgcgac
    27421 gccgcgggtg cgccggtgga aggctttcat cagccggtgc gcgtagcggc cctggcggcg
    27481 gggccgctgc gccccgagca atggggcgag gccgtccgca ctgtggactt cttcgacgtg
    27541 aaggcggacc tggaggcgct gctccatccc cgcgtcgcga ccttcgaacc ggtggtccat
    27601 ccggccctgc atcccggccg cgctgcccgg gtgttgctgg agggccagga gatcggcgtg
    27661 atcggcgaac tccatccggt gtgggtgcag cgctacgagc tgggcactgc gccggtggtc
    27721 ttcgaggtgt cgctcacgtc gttgctggag accatgacgc ccgcctatcg cgaagtctcc
    27781 cgctttccgg cggtgacccg cgatctggcc ctggtggtgg atcaggcgac gccggtggcg
    27841 agcctcattg caggactttc gagtgtcgcg ccggccatcg tccaacgtat cgagttgttc
    27901 gatcaatacc agggcaaagg tatcgaaccg aaccggaaaa gccttgcttt tcgcgtagtt
    27961 atgcaagaaa ctcagaggac tttggaggac gctgaagtgg aagccgcaat gaccgccctg
    28021 acccgccacg ccgaggaagc cctcggtgcg aaactgcgag ggtgatcacc atgggcgtga
    28081 ctctgaccaa ggcggaactc gccgacatgc tgttcgagca ggtgggtctc aacaagcgcg
    28141 aagccaagga catggtggag tgcttcttcg aggagatccg cctggccctg gagcggggcg
    28201 actgcgtgaa attgtccggt ttcggcaact tccagctacg cgaaaagccc cagcgtcccg
    28261 gccgtaatcc caagaccggc gaagagattc ccatcacggc ccggcgtgtg gtgaccttcc
    28321 acgccagcca gaagctcaaa gccaacgtgg agcatctgag tgatgtcgca aagtgaggta
    28381 acggagttcg tcgagctgcc gccgatcccg gccaagcgct atttcacgat cggtgaagtg
    28441 agcgagctgt gcgcggtcaa accccacgtg ctgcgctact gggaacagga attcacccag
    28501 ctgcggccgg tcaagcgggg cggcaaccgc cgttactacc agcaccacga ggtgctgctg
    28561 atccgccgca tccgtgagct gctgtaccgg gacggcttca ccatctccgg cgcgcgcaat
    28621 cgtctcgacg agatggcctc acgtaagcgg gaggacgtgg aggagtccgc cgtcctgcgc
    28681 gatcagctcg cgtcgatccg tggtgagatc caggccatcc tgaaggatct gcagggctga
    28741 ggacgcggcg ggtgacgatg cgctgaaggc ggggtgagtc cccgcctttt ctttttgggt
    28801 gactggtctg acaggctacg gaatggcgaa cagggcatgg cgcgccgtcg ttgcggaggg
    28861 tcgataacgg tccgctgacg ggggcggcga tcgcgttcgc aaccggccga tgcggggccc
    28921 gactaccagc cctgggcggt cttgcgggcc tgctcacact cggccctcag ttccaggaac
    28981 tgggtgaggc ggcgtggcgc gatacggcca tcggccaccg cggcacgcag ggcgcagccc
    29041 ggttcactgt cgtggcggca gtcgcggaag cggcattggc cgtcccaggg gcgaaactcc
    29101 cggaagctgg cttcgagggc ggcattgtcc aggtgggcaa ggccgaaggt ctgcatgccg
    29161 gggctgtcga tcaggccgcc gccgccgggc aggtcgtaga ggcgggcgaa ggtcgtggtg
    29221 tgcttgccgg tgtcgaggac ttccgagatt tcgcgggttt cggcacgggc ctcgggaatc
    29281 agggcgttag tgagggtgga tttgcccatg ccggactgac ccacgagcag gctggtctcg
    29341 tccttgaggc gcgcccgcag ggcgtccacg tcgtgtaggg cggagagctc caggatcggg
    29401 tagtccagcg cggcgaaggg ggccagcagg gtgcgggcgg cgtcgagccc ggcgggcagg
    29461 tcggccttgt tgaggacgat caggctcttg agctgctggc tttcggcggc gcagatgcag
    29521 cgggtcacga aatcgtcgct gaagctgggc tcggtggcca ccaccacaac cacctgggtg
    29581 gcgttggcgg cgatgagctt ctggcgccag gcgtcggagc ggtagagcag gctgccgcgt
    29641 tcttcgagtt gctcgatgac gccctggccg ggcgcgctgc gggccacctg gacccggtca
    29701 ccgcaggcga agcggctctt tttgccgcgg ggtacgcaca acaggatctc gccgccgtcg
    29761 gtgcggactt cgtactgccg cccgaaggcc gccaccaggg tgccgcgggc cttcaccgga
    29821 cagccgcctc gaggcgcgcg atgcggaccg ccgccggtgg gtgggagtca tacacgcggg
    29881 agtagagcgg gtccggcgtg agcgtcgagg cattgtcccg gtacagcttg accagcgccg
    29941 agatcaggct ggcggccggc gcctggcggg cggcgtaggc atcggcctcg aattcatggc
    30001 ggcgcgacca gtggctcatc agcggcgtga ggggaaaggt gaacaccggc agggctagcg
    30061 agaacaggac cagcgtgggc gccgcaccgg tcgcggtcat gcccagcccg gtgaagaacc
    30121 agccttgctg cgccagggcg cccagcagcg cgaagaaggc caggctgccg gtgccgatca
    30181 cggcgaggcg tttccagaga tggtggtggt gatagtggcc gagctcgtgg gcgaggacgg
    30241 cctccacttc ggccgggtcg agcttttcca gcagggtgtc gaagaagacg atgcgcctgg
    30301 aaccgccgaa gccggtgaag taggcgttgc cgtgggccga acgcttggag ccgtccatca
    30361 cgaagaggcc cgagaggcgg aagccgcagc gcgtcatcag ggcctcgatg cgggttttga
    30421 ggtcgccgtc ttcgaggggc tggaagcggt tgaagagggg cgcgatgaag ctggggtaga
    30481 tgagcatcgc cagcaggttg aagccgagcc acgccgccca cacccacagc caccagcgct
    30541 cgcccatggc gcccatcagc cacagggcgc cggccagaag gggcagtccg atcagggcgg
    30601 cgagcagggc gcctttcagg gtgtcggcga cgaacaggcg gggcgtcagg cggttgaagc
    30661 cgaagcgggc ctccagcacg aagctgcgat agagaccgag gggcagatcc accagccagg
    30721 aggccactgc gaccagggtc aggaaggcga cgccctgacc caggctgccg gcgtcgagga
    30781 caccggcgct gaggtcgaac aaggcctgca gcccgccgcc cagggtgagg atcagcagcc
    30841 agacggtgga caccagcagg tccacgagcc ccaggtgcac ccgtgccacc gtgtagtccg
    30901 ctgccttgcg gtgggcatcg aggccgatgg ccgtggcgaa ggcgggcggc acggcgttgc
    30961 ggtgggcgct cacgtggcgg gcgtggcgta gagccagcgg gatgcgtacg gccaggccgg
    31021 caagcacggc cacgaggaac agggtggtga aactgctggt ggacatcgat gaggggcggg
    31081 caggcagggg agggttggat cggaccggcg caggacggtt cctgccgtca gggcagggga
    31141 gtgagcgggg cggcggcgag ccgcaagttc ggcacggact gcgcgacgag gcgccggacg
    31201 atgtgccaga atcgcgcctt taccgacgag cgagccgatt atggcacagg acccgaacca
    31261 cctcatctgg ctggacatgg aaatgaccgg tctgaatccc gacaccgacc gcatcatcga
    31321 gatcgccatg atcgtcaccg acagccagct caacctggtg gcggaaggtc cggtggtggc
    31381 ggtgcatcag cccgatgcgg tgctggaggc catggacgac tggaacaaga acacccacgg
    31441 ccgctcgggc ctgatcgacc gggtcaaggc ctcaaccgtg gacgaggcgg ccgccgaagc
    31501 cctgttcctg gatttcctgc aggcccacgt gccggcccgg acctcgccca tgtgcggcaa
    31561 ctccatcggc caggaccgcc gcttcatggt ccgctacatg ccgaagctgg aggacttctt
    31621 tcattaccgc aacctggatg tgtccaccct caaggaactg tgcaagcgct ggcggccgga
    31681 cgtctacgcc ggcctcacca agcagggcgc ccaccaggcg ctggcggaca tccaggaatc
    31741 catcgacgaa ctgcgttatt accgggcgaa cttcctgaag gtctaggatg cgccgacgtc
    31801 cgtccacgca gtcggctcgg catggggcga cggaacaccc cgtttcatgc gccccggctg
    31861 caggctgccc agggtacagc cgatgcgcca cctgaaatga gccgcgacag cctgcgtgaa
    31921 acgtggcgga agcatcgccg tatggtgatc ggcgtggccg tgctgtgtgc cttcttctac
    31981 gccctggggt atttctcacc gcgggtcgcg tcgcccctca tgtgcaagat ctacggctga
    32041 aacgcccgcg ccgtcgacgc ggcacagggc ccgcaaccgg ctgcgggcgc gggccagatc
    32101 cttgccgagc agaccgtcca gttcggcacc gatgccggcg acccaggttt ctggtgcggc
    32161 ctcggccagg acgcccaggg ccagacctgc gtcccaggcg gcatccaggc accagacgag
    32221 gcggcgcagg gcgtcgccat cccgcggggt gaggcgggga aagccgtcga tcagcagacc
    32281 gccggcggtc tcggccaggc ggacgtagtc tcgctggggc cggggctggc gaaagagctc
    32341 gtcgaaatcc cgggcgcatc cggcggtggc ggtcagccag cgtcgggcgg cctggcgatc
    32401 ggtggcatcc gtcgcggacc accagcgcag cgggccatgg gcggcgagca attcccgata
    32461 atcccgtccg ccgtccacct cgagcagcag gcactgggcg gccagccgct gcgccaccgg
    32521 tgcaaagtga tgccgccgcg cgcggtgttc cggtgtgtcc ggccacaggc ggtccggcgg
    32581 gtgattggcg gtgacgacgc agaccatgcc ccgctcggcg acgcccgcca gcaggcgctc
    32641 cagcaccagg gcgtcggccg ggtcgtaggc atggaattcg tcaagtagca gcacgcgggc
    32701 gctgccgaac agggtgtcga gccgtgccgc ccagtccggc tggcagacac aggtggcgtg
    32761 ataggcgcca agcagggcgt tgagggggta acggcggatt gctgcaggcg gcagtaccgc
    32821 gtcgaggcag gccatcaggc gcgtcttgcc tcgcccgaca ccgccccaca ggaagagccc
    32881 gcgggtcgcg cgtcggtgga caagggcgtc ggcgaggcgc gccagatggt cgagcgccgc
    32941 gccctgcgcg gtgtcgggga tcgggccgcc acgccggaaa cgggcttcga gggcgacacg
    33001 caggggcgtc atgccagcgc cttgcccgcc agggcgaggc cggcgacgat gagaaggccg
    33061 tcgatcaggc gtccgagggc ggggccggag aggcggcggt cgatccgctg acccagggtc
    33121 aggcccagcc aggcggcggg caggagcagg gcggcggtga cgagcaggcg gccatccacg
    33181 agcccggagg ctgcacacag ggcgatgcgc caggcggtgg acagggcgat cagcacgccc
    33241 tgggtggcgc ggaaggccgc ccgctcgagg cgcggctgca ggtaggctgc gtaggcgaag
    33301 ccgccgctgc caaatactcc gcccagtacg ccgccaaagc cgccatacag ccatgcccgg
    33361 gtgggggtgg cctgctccgc gccgcggcga ccgagcgcat gccagacgcc gtagccggct
    33421 acaaagcctc ccaggagcag ggccaacccg tgggcgggca gggcgctcag cagtccgaca
    33481 ccgacgatct gtccgagcag catgccgggc agcaggcgcc cgagggccgc ccgatcgacg
    33541 gtcgtacgtg cctgccacgc ccggctcacc gaaccggcgc aatcgagcac cgccagcagg
    33601 ggcacgatgc gtgccaccgg cagtacccag gccagcggcg cgctggcgat cagcgccgag
    33661 ccgaagccag ccagagcaaa gagggcgtag gcgatgacca ggatcgccgc ggcgcaggcc
    33721 actccggcgg cgtcgccaag gggcacatcg gggagcaggg ccatgagcgg aggcacgggt
    33781 gaggccaggt gttgcgatgc tggtattgtt ggcccggcag acgtataacg ccaatatcaa
    33841 aaatcctgct tatccatgcc tttttgcgat gcttagtctg cgccagatcc gctatttcca
    33901 ggccgtggcc gacaccggca gcttcacgct ggcggcggcg caggtgtaca tcgcccagcc
    33961 tgcgctcagc cggcagatcg ccttgctcga agcggaactg ggcgtggcgc tcttcgtacg
    34021 ggaagcgcgc ggcgtgcggc tgaccccggc cggacagctg ttccgggccc gcacggagaa
    34081 cgtcgggacg gcgctggagg cggcggtgga ggacgcacgg cgcctcgacc ggggcgaggc
    34141 cggtgtgttg cgcctgcttc attccagttc tgtgcccctg gccggcccct tgctggaggc
    34201 cttgcgatgc ttgaccgacg cggcgccggc ggcccgcgtg gagctggacc gggtgtcctc
    34261 cgaggtgcag gtggcggaga tcgccgccgg gcgggcggac gtgggggtgg cgcggatgcc
    34321 ggtgctgcgc cgggaggccg cagtggcgtt cacggcgctg ccggaggagc ggctggtgct
    34381 ggcggtgccg ggggctcatc cgctggccgg gcggacggca gtgagcctcg ccgagctggc
    34441 tggcgagacc ttcgtgacgg cggtccatcg ggagcggggc ggactggccc ggcgggtgac
    34501 ggacctgtgc ctggcacggg gcttcgtgcc gcgcagcggt gcggtggtgt cccgcaagac
    34561 gtccatgctc aacctggtgg cggccggcta cggggtggcg gtggtgccgg cgggcatggc
    34621 cgggctgctg ccggccggtg cccggctggt ggcgctgacg gacgcggagg ccacgtccgc
    34681 gtcggccgtg ctgttgcctc tggcgccgtc cccgctggcg cggcgctttg ccgaactgtg
    34741 cggggcggcg tgggccggag tggccggggc gggctgctag ccgccccgca gccagcggcg
    34801 gctgctctcg gtgtcgagca ggtcgcgggg ggtgggtagg ttgaaatgcc gggtggcggc
    34861 cagggccacc tgatggagct gggcgacgac caggctttcg aggagggcgt cgcggctgtc
    34921 gtagcggtcg atgccgaagc tgccgagcca ggcgtccatg cgcgcctggc cgccgatgcg
    34981 ttcggggtag aggctgggca gcatgaccat caggtcgatc cacgcgagct cgggcagcgg
    35041 gcccaggtgc tgctcgaagg cgcgctccag actgccccgg ttgaaggggg cgttgaagac
    35101 caccaccggc gcgcgggcga tgaaggcgag gagcccgatg agggcgtcgg cgggtgtgtc
    35161 gccgaggcgg gcgtagtagg cgtccgccgg atggatcagg ccgcggttga gggccacggc
    35221 gcctaccgcc agcaggcgcc gtccgccatc ctcgaccggg ccggtctctg cgttgacggc
    35281 cacgtagcgg gtctcaaaat ggacgcggcc aagatccggt gcaggcaggg cggccaccgc
    35341 gtcgagggcg gccttctgat cgggcgtgag gggcgttccg ccgccgcccg gcaggaatcg
    35401 ggtgagccag ttcatatctg gtagtccagc ttgaggcgca gttgcagttt gcgggcctgg
    35461 cggaaggctt ccttcaggat gcggcggtcg agctcgttga ggtcgtcagg cttgacccgg
    35521 ttgtcgccgg gcgcgccgtg gtcggtgtcg agatgctggt ggcgcaggcg caggagctgg
    35581 atgaagtgga agccgtcggc catcgcggag agttcttcgg cgggcatgcc gatgcggctg
    35641 ccgccctggc gcaggcgctg gaccgtggcg gtggcctcga cgccggtgcg cagggccagc
    35701 acgcgggcgg catccacgaa gaggcgggcg ccgaatttct tgaggtcgat ggtgcccgga
    35761 tatttgggat cgtcatccac cacgaaatcg cggatcttgc ccagaggcgg cgccacgtcg
    35821 agggcgttgg tggccagcga cttgaggaag gccggattgc cgccggtgag gctgagcagc
    35881 cactggcgca gcttgtgggc gagcttttcg tcgccgtaga gcacgcggta gtcgaagaag
    35941 atggtggcgt tgagcagggc ttcggggtgg ggttcgcgga tccactggcc gaacttctcg
    36001 cgccattcgt cgaaggtcag gcacagctcc gggttgctgg ccatgatgtt gccctcgcac
    36061 agggggaagc cgcaggcggc caggtcgttg ttcacgtcct tggcgaaggc cagcaggcgc
    36121 agcttgaggg gttccttgtc ggcccattcg ggcagcgtga agaggatccc gttgtcctgg
    36181 tcggtggaca gggtctgttc gtggcggccc tcggagccga aggcgagcca cgcgtattcg
    36241 atgccttcca ggtcgtggcg ctgcaggttg agctcgacga tgcggcgggt gagggcgtcg
    36301 ttgagggccg agatgaactg ggtgagctgc tcggcgccga taccctgggc cagcaggttg
    36361 agggcgagct gacggatgtc gcggctggcg cgctgcaggg cctcgatgtc ggccgcgctt
    36421 tcgatgccgg agcggatctg gcgcaggccg atgcgctgca gggcgaagag gtcgcgctcg
    36481 gagatcacgc ccttgagctt gtcctcggta tccaccacga ggatgtggcg cacgccgtgg
    36541 gtggccatct cgagggcggc gtcgtaggcg ctggcggtgg cgtggagggt gtggggcggg
    36601 cgggtcatca cgtcgccgat ggggcgggtg aggtcgaagc caggcagcac gatgcgcttg
    36661 aggatgtcgc tctgggtgag cacgccgacg ggcttttcgt cctcgccagc caccgccaga
    36721 cagccgacac cggcctcgac catcttttcg agggcgctgc gggtgggcgt ctcgggcccg
    36781 acgaagaccg gcgccttggt ggcgagctgg gccagggagg tggtcatggt ctgctgttcg
    36841 gccgcacgct gggcgaactg gacctggagc tgctcccgcg actggttgag caggctggcg
    36901 atgtagcggg tgcagaagag ctggaagctc gggctgatct ccagcaactt gaggaagtcg
    36961 gcggcgggga gctggaagca gtagctgtct tccagggcca cgtaggcgtt ggtggacggg
    37021 cgctccgccg aaatcgcacc aatcgggaag ccttcgccag gacccagggt gagggtggaa
    37081 tactcggtga cggtgacgcc gacctggcgg gcctgcacct tgccgcgata gacgatgtag
    37141 aagaagcgcg gtgggccgtc ctcgggcatc aggacttccg cctcggccgg gtagtaggcg
    37201 gtggacagcc gttcgccgag gaagcgcagg gcgtcgggct ccatgcggtc gaagggcgca
    37261 tggcgacgca ggaaatccag ctgggtgcgg atcagggtat cggaagcgga gggagtatcc
    37321 ataacggcca tcactgacgc gatcaaaccc atgaatctac tcttccgctg ccctcccgtc
    37381 atgattttcc tcaacattcc gcgtggtttc gaggcaaaag gaggccctcg gctagaatgc
    37441 gggctcccct tctccggaca tcgcccatga acgccgccct gctccctgtt gtcgagattg
    37501 aaccggctgt gccggcaacc cggtcggtga tctggctcca cggcctgggc gccgatgggc
    37561 acgacttcga accggtggtg gacgagttcg acctggaccg tctgccgccg acccgcttcg
    37621 tctttcccca cgcgccgatg cgcgccgtga ccatcaacgg cggctatgtg atgcgcgcct
    37681 ggtacgacat cctgtccatg gatttttccg agcggcgcga ggatccggca ggtgtgctgg
    37741 aatccgccgc ccaggtggag gccctgatcg cccgcgagaa tgcccgcggg atcgccgatg
    37801 gacacatcgt gctggcgggc ttctcccagg gcggcgcgat cgccctgcac acggccctgc
    37861 ggcacccgcg ccgtctggcg ggcgtgctcg ccctgtccac ctatgtgccc ctggccgata
    37921 ccctcgccgc ggaagctgcg ccggccaacc gggatgtgcc cctcttcatg gcccacggcc
    37981 tgggcgatcc ggtgatcccc tacgccttca gccgccgctc gggcgatctg ctggcggacc
    38041 tgggctatcc gctggaatgg cacgactacc ccatggacca cgccctgtgc ctggaggaag
    38101 tgcggcacat cgaggactgg ctcgcccgcg tcctcgcctg acacgacggt acgcgccgat
    38161 caccccgccg ggcggcggcg cagcaggtgg gcgacgatga agctgaggac ggtctcaccg
    38221 cgctgattga ccgccgtgta cttgaagcgg gcgatgcccc ggtccgggcg cgtggacgag
    38281 gggcgcacct ccagcacgtc cacctcggtg tgcagcgtgt cgcccgggcg caccggggcc
    38341 agccagcgca gctcgtcgat acccggcgag cccatgctgg actccgccag gatgccgctc
    38401 tggatgaaca ggcggaagca cagggcgagc gactggaagc cgctggcgat caggccgccg
    38461 tagggggatt cggccgcggc gacggcatcc agatggaagg actgggggtc gtagcgcagg
    38521 gcgaagtcga tgatctcggc ttcggtgagg gtgatgcccg gcgaggtgaa gcggtcgccg
    38581 ggattcaggt cgtccaggaa gcgggtgtcc atggcagtct cctcgttggg ggtcggcgca
    38641 tcttagccga tggcgccgcc gtcaaggacg tcctcgcaca ccgccagcca ggcccgggcg
    38701 gcgtgggaca ggtagcggcc gggtggccag atgtgggcca ccgtccacgc caacgccgga
    38761 tcggccagcg gccgcacggc cagctccgga tgcagggcca tgcgggccag caggggctgg
    38821 ggcaacaggg cggtgcccag gccggcggcg gccagggcca ccaggaagtc ccactggccg
    38881 ctctgggcca cgatgcgcgg ctccacgccg aagcgacgga aggcgtcgcg cagctggcgg
    38941 gtgagggcaa agtcgtcgtg gaggaggacc agcgcctccc cgtccagatg gctcaggtcc
    39001 acctgcgcct gccgggccca cagcgcatcg gcaggcccca ccgcgacgat gggatggcgc
    39061 ccgacggtcc gcgtcgccag accgagggcc gggtccaccg gcagcagggt cacgcccacc
    39121 tccagttcgc cgctcgccac ctgctgctcg atgatctgcc cgccccgttc cacgagggtc
    39181 aggcgcacgc ccggatggcg ttcccgaaag gccttgacga cgggggtgaa gaacagattc
    39241 acagtggggg gcagacccac cgtgagttcg ccgcgggcga gggcggtgag gtcggcgacc
    39301 tcccggttca gttcgcgcat cacgcccagg gcttcctgac cgcggtcgaa gaggatgcgc
    39361 cccacatcgg tgacgcggat gcgccgccca tcccgatgga gcaggggctg gccgacttcg
    39421 tcctccaact ggcggagcat cttgctgacg gtggactggg tgacgcagag acgctcggcc
    39481 gccacggtga agctgccgtg gcggacggtc tcgatgaagt agcgcaaggc ccgcaggtcc
    39541 atctatgtat gaacgaaacg aatgatgtga agtagtttaa gtcatccagt gcatgtacgc
    39601 gtctccctat actcgtctcc caatgagggc catccaatcc ggccccgccc tggagaggag
    39661 aaaacatgcc cctggatcgc atccgtctgc cccgcctgcg tgaacgcatc atgtcccccg
    39721 aggaggccgc gagcctgttc cgggacggca tgaccgtggg catgagcggc ttcacccgcg
    39781 ccggcgactg caaggccgtg cccttcgcgc tggccgagcg ggccgcccgc gagcccctgc
    39841 agatcaccct gatgaccggc gcgtccctgg gcaacgatat cgacaaggtg ctgtcggaag
    39901 ccaacgccat cgcgcgccgc atccccttcc agtccgaccc cagcctgcgc cgcaagatca
    39961 acgcgggcga ggtgatgttc atcgaccagc acctctccga gaccgtcgag cagctgcgtt
    40021 ccggccagat cgcgccggtg gacatcgccg tggtggaagc ctcggccatc accgagaacg
    40081 gcggcatcgt gccgaccacc tcggtgggca attcggcgag cttcgcggtg ctggcaaaga
    40141 agatcatcgt cgagatcaac ctgaagatgc cggtcgagct ggaaggcctg cacgacatct
    40201 acatcccgac ctatcgtccg caccgcctgc cgattccgct gatcgcgccg gagcagcgca
    40261 tcgggctgcc ctacatcccg gtggatccgg agaagatcgt cgccatcgtg atcaccgaga
    40321 aggccgacag cccgtccaac gtgctgccgc ccgacccgga gaccaacgcc atcgccggcc
    40381 acctgaacga gttcctctac cacgaggtca aggccgggcg cctgtcgccc agcctccagc
    40441 ccctgcaggc gggcatcggc accatcgcca acgcggtgct gcacggcatg ctggattcgc
    40501 ccttccgcga cctcaagatg tactcggaag tgctccagga cagcaccgtg gagctcctcg
    40561 acgacggccg cctgagtttt gcatcggcgt cctccatcac cctgtcgaag gccacctacg
    40621 agcgcgtgct gggccagatc gagcgttaca agcagcgcct ggtgctgcgt ccgcaggaaa
    40681 tcagcaacca tcccgaggtg atccgccgcc tcggcctgat caccatcaac accgcgctgg
    40741 agtgcgacct ctacggcaac gtgaactcca cccatgtggg cggcacccac atgatgaacg
    40801 gcatcggcgg ctccggcgac ttcgcccgca atgctcacct gtcggtgttc gtgaccaagt
    40861 ccatcgccaa ggacggggcc atctccagca tcgtgcccat ggtgtcccac acggaccaca
    40921 ccgagcacga cacggacatc atcgtcaccg agcacggtct ggccgacctg cgcggtctgg
    40981 ccccgcggga gcgggcccgc cagatcatcg accactgcac cgatccgctg tatcgggatg
    41041 ccctgcggga ttacttcctg cgggcctgcc gacgcggcgg ccagaccccg catctgctgg
    41101 aggaggcctt ctcctggcat ctgcgctatg cccgcgaggg cgccatgctg gcgcgtgacg
    41161 gtcgcgtcga gcccgcctga gaacccgctg gcgcgcgtgc aggccgcgcg cccggcgccg
    41221 tcggcagcct tcggccggcg gcacgcttga ctgcctgctt ctctcccccg aggccccccg
    41281 cgttccgcgt cccccgcgcg gaacgcggcg tgggtacgcg atccgccgtg cctcagcgtc
    41341 ggacgcgcac ctccgcggcc ccgtcgtgca tggccccgat caccgccgcc tcggcgaagc
    41401 cgtgctgccg gaacacctcc agcaccgccg gcaccgcctc cgccgcgcag ctcaccagca
    41461 ggccaccgct ggtctgggcg tccgccacca ggtggcgctg ccagtccgcc acgtcctcgt
    41521 cgaaacgcac cgcgtcgccg aagctcgcca gattgcgggc gatggcgccg gggccgatgc
    41581 cctgttgtgc caggggcacg gcggccggca tcaccggcag acgggcggcc tccacggtgg
    41641 cgcccaggct ggcgccgcgg cacagctcca gcaggtggcc aagcaggccg aagccggtca
    41701 cgtcggtgag ggcatgcacg ccgggcagct gcgccagcac cgggcccggc gtgttgaggc
    41761 gggtggtggt ggcgatcatc tgggcgtagc cgtccgcatc cagcaggccc ttcttcatgg
    41821 cgctgccgag gatgcccacg ccgaggggct tgcccagcac cagcacgtcg ccggcgcggg
    41881 cgtcccggtt gcgcttgatc acgtccgggt gggcgaggcc cagggcgacc aggccgtaga
    41941 tcggctccgg tgcgtcgatg ctgtgcccgc cagccaccgg aatgccggcg tcggcgcaca
    42001 cactggcgcc gccggcgagg atgcggccga tggcgtccag aggcagcttg tcgaggggca
    42061 tgccgacgat ggccagggcc aggatcggcg tgccgcccat ggcgtagacg tccgacaggg
    42121 cattggtggc cgcgatgcgg ccgaaatcga aggggtcgtc cacgatgggc atgaagaagt
    42181 ccgtcgtggc gatcagcgcc tgggtgtcgt tgagccgcca cacggccgcg tcgtcggcgg
    42241 tctcggtgcc caccagcagg ttggcgaagg gctgggccgc gggcatgcgg gccagcatct
    42301 cggtgagcac gccgggggcg attttgcagc cgcatccgcc gccgtgcgac aatccggtga
    42361 gccgcagagg tgtatccgtc atctcgttgt cgtcctgact ttttccatta cccatgtccg
    42421 tgtcgcccca tgcgccgcta tcggtcggtc tcgaccggct ggcgcacttc gacgccgtca
    42481 tcgacgtgcg ttcgccttct gaattcgccg aggatcacat ccccggcgcc ctcaactgtc
    42541 cggtgctgga cgacgaggag cgtcgccgcg tgggcaccct gtacaagcag gtctcgccct
    42601 tcgaggcgcg caagctgggg gccgcgctgg tgtcgcgcaa catcgcgcgg catctcgaga
    42661 cgcattttct cagcaagccg aagggctgga aacccttggt gtgctgctgg cgtggcgggc
    42721 agcgcagcgg cgccatgacc ctggtgctca accaggtggg ttggggcgcg cgccagctcg
    42781 ccggtggcta caaggccttc cgccagcagg tgatggacga tctggtggcg ctgccggagg
    42841 ggctgtcctt ccgcatcctg tgcgggccca ccggcagcgg caagaccgcg ctgttgcggc
    42901 gactggcggc gcagggcggg caggtcctgg acctggaagg cctggcggtg cacaaagggt
    42961 cggtgctggg gggcgacgtg gtgcgcccgc agcccggcca gaaggccttc gagacgggcc
    43021 tgtggcaggc cctgcggggc ttcgatccgg cccgccccgt gtgggtggag gcggagagcc
    43081 ggcgcatcgg gcgcctgcac ctgccgccgg aactcttcgc gcgtctggcc gatggtcgct
    43141 gcaccctcgt gcgtgcgccg ctggaggcgc gggtggccta cctgctgcgc gactacgccg
    43201 atctgatcgc cgagcctgcc gccttcggcg cccgcctgga gcgcctgatc ccgctgcatg
    43261 gacacgagcg ggtgctggcc tggcgggcgc agatcgacgg tggcgactgg gagaccctcg
    43321 cccgcgagct gatcgtgacc cactacgatc cggcctaccg gcgcggcggg gacggcctct
    43381 accggcacgt ggcgcaggcg gccgtgctcg acgcggcgtc gctggatgcc gatgcgctgg
    43441 acgccctcgc gcgtggcctg gccaacgcca tctgaccgac cgtgaacgca cgaccggccg
    43501 cgcagggcgg ccggccgacg tgacggcaag gtgaacgtcc tacaacactt ccgccgcgtg
    43561 atccgccagg cgcgagcgtt cgccccgttg cagggtgatg tggcctgagt ggggccagcc
    43621 cttgaagcgg tccaccacgt aggtcaggcc cgacgaaccc tcggtgaggt agggcgtgtc
    43681 gatctgggcg atgttaccca ggcataccac cttggtgccg gggccggcgc gggtgatgag
    43741 ggtcttcatc tgcttgggcg tgaggttctg ggcctcgtcg atgatgagaa acttgttgat
    43801 gaaggtgcgt ccgcgcatga agttgaggga cttgatcttg atgcggctgc ggatcaggtc
    43861 gcgggtggcg gcgcggcccc attcgccgcc ttcgccggtg gagccgttga gcacgtccag
    43921 gttgtcctcc agcgcgccca tccacggcgc catcttctcc tcctcggtac ccggcaggaa
    43981 gccgatgtcc tcgcccaccg gcacggtgac gcgggtcatg atgatctcgg agtagcgctt
    44041 gctctccagc acctgggtga ggctggccgc cagggtgagc agggtcttgc cggtgccggc
    44101 ctggccgagc agggtgacga agtcgatctc cgggttcatg agcaggttga gggcgaagtt
    44161 ctgctcccgg ttgcgggcgg tgatgcccca cacgttgttc ttctggtggg aatagtcggt
    44221 cagggattca agggtgatgc tgcgcccggc cttgtccttg acccaggcct ggagcggctg
    44281 ctcgccttcc tggaagacga attcattgat cagcaggtcg gcggtgtccg gacccttgat
    44341 gcggtagtag gcccggcctt cctgtttcca ggactccatg tccttgccgt tgcgctccca
    44401 gaacggggcg tcgatctcct tgacgccggt atacaggagg tcggtgtcct ccagcacctt
    44461 gtcgttgaag tagtcctgcg ccgccaggcc cagcgcgcgg gccttgatgc gcatgttgat
    44521 gtccttcgag acgaggatca ccgggcgctt ggcgaatttt tcgtggaggt gcatcaccac
    44581 cgccaggatc tggttgtcgc ccttggcggt gggcagcgcg gggaggcggg tgtcgatggc
    44641 ctcggtctgc aggtacagac ggccgctggc aagctggctg gaggcctgtt ccagggagat
    44701 gccggcctcg atgccgtcgg ggctggagtt gacgatctcg tcaaggctgc ggctggcctg
    44761 gcgggcgttg cgggcgactt ccgacatgcc cttcttgttg gagtccagct cctccagggt
    44821 catcatcggc acgtagatgt cgtgctcctc gaagcggaag aggctggtgg ggtcgtgcat
    44881 cagcacgttg gtgtcgagaa cgaacagctt ggtgatggcg gctttcttgg tcgttcggcg
    44941 ggtggccatg gtgggcggag cctcgggctg gagttaggcg gcggtggaga gcgtcttcac
    45001 atagtcgagc acttcctggg cgtggccagg caccttcacg ccgcgccatt cacgcgtcag
    45061 gacgccctgg gcgtcgatca cgaaggtgct gcgttcgatg ccccgcacct gtttgccgta
    45121 catgtttttc agtttcatga cgccgaacag ggtacaggcg gtctcgtccg gatcggagat
    45181 caggtcgaag ggcagggcct gtttggcgcg gaagttgtcg tgggacttga ggctgtcgcg
    45241 ggacaggccc agcacgaccg cgccggccgc caggaactgg tcgtgcaggt cgcggaactg
    45301 ctggctttcg gtggtgcagc cgggggtgct gtccttgggg tagaagtaca ggacgacgct
    45361 gcggccccgg aactgggcga ggctgagggt ctggccgccg gtggcgggca ggctgaaatc
    45421 gggtgcggcc tggtcgagca tgctgactcc ggaagtgggg tgaggtccgg tggcagcgta
    45481 atcccaaggc gccggccttg gcaatcggcc cggaggccgg cgcggcgcgc ctcaggccgg
    45541 atcgaagagc agggcggccg ccacggcccg gtgttcggcg acgagcaggt tgtaggtgcg
    45601 gcaggcggag cccaggtcca tcacctcgaa gccgatgcgc gcctgcatca gcggacgcag
    45661 caactgcggc gccgggaagc gctgacgcag gccggtgccg aggatgacga tctcggtacc
    45721 gagcccggcg aggcgggcga aggcggcctc gtcgagggca tcgaagccac ccatgtgcca
    45781 gccggcttcg atacggtcgg gcaggacgat cacgctgcct tcgtagcgtt cggcgccgac
    45841 ccgcacgtgg gtcgcgtcgt aacgggagat ggtgttgtac tgctcggctt tgtccagatg
    45901 cagtttcatc gcgggggagt ctctccgggg gcggtcggtg gggcgggctg atttgcccgg
    45961 tcaggggcgc ttgggtaaca ttataacttt gcgtcgccgc gtacctgccg ggtccgtccc
    46021 gtcgccgcgg gggcccgcct cccccagcat gagccgccac gtccgcccgc gcgtaccgtc
    46081 ccgcgccgcc ccgaagagcg tttcgcccag caaggaaagc accgtgcaac ccatcctcaa
    46141 gtccgacaaa ctcgccaacg tctgctacga catccgcggc cccgtgctgc agaaggccaa
    46201 gcagatggag gacgaagggc acaagatcat caagctgaac atcggcaacc tcgccgcctt
    46261 cgggttcgac gccccggagg agatccagct cgacatgatc cgcaatctgc ccaacgcggc
    46321 aggctactcg gactccaagg gcatcttcgc cgcccgcaag gcgatcatgc actacaccca
    46381 gcagaagcac atcaagggcg tgacgctgga agacatctac gtgggcaacg gcgtgtccga
    46441 gctcatcgtg atggccatga acgcgctcct caacgctggt gacgagg
//
LOCUS       DAOQMM010000015        46431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_183_length_46431_cov_49.868186, whole genome shotgun sequence.
ACCESSION   DAOQMM010000015 DAOQMM010000000
VERSION     DAOQMM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 46431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46431
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_46431_cov_49.868186"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..634)
                     /locus_tag="PLH85_04280"
     CDS             complement(2..634)
                     /locus_tag="PLH85_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNB63805.1"
                     /translation="MYLPPLFREDDPARCAELINAHPLATLISQGPGGLQANLVPVLF
                     YPDEGPHGTLRAHLARPNPQLAELAAQPDCLVVFRGPEAYITPAWYPSKRTTGKVVPT
                     WNFATVHAWGPARLIDDPAWLLRLVTDLTDHQEAPRATPWRVDDAPADYISAQLKAIV
                     GLEIPVQRLEGKWKMSQNRSPADRQGVVDGLWDAGEHAVAEAVAQRMRGE"
     gene            complement(699..1130)
                     /locus_tag="PLH85_04285"
     CDS             complement(699..1130)
                     /locus_tag="PLH85_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNB63806.1"
                     /translation="MFDPSRDQARQFFLDAWRKYRNRDVLTQLETLAADLVAHHPEYH
                     PLLEDADAVHKDFSPEDGQINPFLHLSLHLAIEEQLSINQPPGIRDAFEQCQARHGDR
                     HAALHDVLECLGETLWRAQRDKAPLDGRAYVDCVRKRGGIS"
     assembly_gap    1137..1236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1307..1939)
                     /gene="nth"
                     /locus_tag="PLH85_04290"
     CDS             complement(1307..1939)
                     /gene="nth"
                     /locus_tag="PLH85_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNB63807.1"
                     /translation="MRLAEIAEAFRRLATANPHPTTELEYASPYQLLVAVVLSAQATD
                     KGVNLATRRLFALAPTPEAMVSLGEDAIAEQIKTIGLFRNKARNTLLLSQQLLERHGG
                     EVPHDRAALEALPGVGRKTANVVLNTVFGDPTMAVDTHIFRLANRTGLAPGKDVLEVE
                     KRLLRRIPKAYLRDAHHWLILHGRYVCVARTPKCGECLLRDLCRFPDKTA"
     gene            complement(1952..2638)
                     /locus_tag="PLH85_04295"
     CDS             complement(1952..2638)
                     /locus_tag="PLH85_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNB63808.1"
                     /translation="MDIKAYRDIAWNGWWKQNPGIVQILGMCPTLAITTNIVNALSLG
                     LATMFVMGMSNLAVAALRNFIPYEIRIPVFILIIAALTTVVDLSMNAFVHDLYLVLGI
                     FIPLIVTNCIVLARVEAFAAKNEPLGSTLDGIAQGAGLTLVLVVLGALREFVGSGTLL
                     SGIEMIIPGASPIVLFGDDYPGFLVAILPPGAFFALGSLVAGRNWLEARKAARARQAP
                     PSIDTPVEAG"
     gene            complement(2652..3311)
                     /gene="rsxG"
                     /locus_tag="PLH85_04300"
     CDS             complement(2652..3311)
                     /gene="rsxG"
                     /locus_tag="PLH85_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNB63809.1"
                     /translation="MSETTALGLSTRTGAVMIAFTVVFTALMAFTEDATRERIAASAN
                     DEKMKSIAEVLPPAAYDNALLDDFILTGPTPALGLDDGGRIYRARKAGQPAALVLEAI
                     APDGYSGKVNLIVAVRADGSLSGVRVTGHRETPGLGDYIDPKKDKNKAAPWIVQFTDL
                     NPASTPLDQWKVRKDGGQIAYHTGATISARAVTNAVARAARFAADNQNRLFAAPSGKP
                     L"
     gene            complement(3317..4339)
                     /locus_tag="PLH85_04305"
     CDS             complement(3317..4339)
                     /locus_tag="PLH85_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNB63810.1"
                     /translation="MLNSPFIRKPASVQSVMLQVLLALVPGIAAYVWFFGAGILVNLA
                     LATAAALAGEAALLTVRGRPRALFLTDLSAVVTAWLIALTFPPIVPWWITVLATLLAI
                     VVVKQLYGGLGQNPFNPAMAAFCLMIVAYPALMSQWPAPGQLDFTTQLDLIFGGARAA
                     DAITGATPLDALRTALKSAANPDSPLYNLPASQLMDGPAFGTLGGQGSEWVALGYLLG
                     GLWLIQRRIITWHIPFAFCAALFAVAGLLWAVKPAGFASPLFHLASGGSMLGAFFILT
                     DPVSGATTPRGKLLFAAGAACLTYIIRAFGAYPDGIAFATLLMNILVPLIDMKTQSPV
                     FGHKKD"
     gene            complement(4364..6070)
                     /gene="rsxC"
                     /locus_tag="PLH85_04310"
     CDS             complement(4364..6070)
                     /gene="rsxC"
                     /locus_tag="PLH85_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNB63811.1"
                     /translation="MLRKLFSFHGGVKPQTHKAQSTTAPIAPVPMPDRLVIPLHQAVG
                     GQPRPLVKPGERVFKGQRIGAADGNVSSAVHASTSGLVRDVAPALMPHTSGLSALSVI
                     LEPDGEDRWGELETINLKSAAPDELRDYLRDAGVVGLGGAVFPSHLKLKPGKVGHTKT
                     LVINGAECEPFITCDDLLMRERAGDMLKGISAMRAMLQAEEVLIGIEDNKPEAVAAVQ
                     AAIAARGEAGIEAVAIPTRYPAGGAKQLIRVLTGIEVPHGRRSTDYGVQCFNVGTAFA
                     IHEALDRARPLVSRVVTVTGNVAHPRNYDVLLGTPMDHVVRLAGPGADTDRYIMGGPM
                     MGLRMPSFDVPVVKATNCILVGSPALFPPRPPEMPCIRCGECSKACPADLAPFELYWF
                     SRSKNFGKAQEYHLFDCIECGCCSYVCPSQIPLVDYYRFAKSEIWARERDKDASDRAR
                     DRFEYKNLRQEREKQEKAEKLAAKAAATREQAAAKPAGDAPATGTAPAAKPSPDDAKR
                     ALIEAAMEKARQQKAQVTPKNTDHLSPGQQADIDAIDARRQAASDAPAAAKADEPPAG
                     SA"
     gene            complement(6119..6688)
                     /gene="rsxB"
                     /locus_tag="PLH85_04315"
     CDS             complement(6119..6688)
                     /gene="rsxB"
                     /locus_tag="PLH85_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HNB63812.1"
                     /translation="MLSALLVMTAIALVLGAMLGFASIKFKVEGDPLVDQIDAILPQT
                     QCGQCGFPGCRPYATAIANGEAEINQCPPGGEEGIRKLSDLLGREFKPLSEEHGVEKP
                     KSVAVIDEHTCIGCTLCIQACPVDAIVGAAKQLHTVVARECTGCELCIAPCPVDCIHM
                     RPIAETVDTWKWRYPVIPLAEAAPADRAA"
     gene            complement(6700..7284)
                     /gene="rsxA"
                     /locus_tag="PLH85_04320"
     CDS             complement(6700..7284)
                     /gene="rsxA"
                     /locus_tag="PLH85_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNB63813.1"
                     /translation="MSSYLLVILGAVLVNNVVLVRILGLCPFMGVSKKLDTAVGMGAA
                     TTFVLTLGCGTSYLIDHYVLSAFGLSYLQTLAFIVIIAAIVQLTELIIQKTSPVLHQV
                     LGIYLPLITTNCAVLGVPLLNVALHHDLLQSLLFGFGSSVGFSLALILFAGIRERLDG
                     ADVPAPFRGTAIAFVTAGLMSLAFMGFAGLDKYH"
     gene            complement(7379..8392)
                     /locus_tag="PLH85_04325"
     CDS             complement(7379..8392)
                     /locus_tag="PLH85_04325"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNB63814.1"
                     /translation="MLYDIARPVLFSFDAETAHEAALAALNVAGRVLPAGKPLDARPV
                     DVMGLRFPNRVGMAAGLDKNGEVIDGLARFGFGFIEIGTITPRPQAGNPKPRMFRLPE
                     VRGIINRMGFNNHGIDALVANVQAARYRGILGINIGKNADTPIERAADDYLACLDKAY
                     PLASYVTVNISSPNTKNLRQLQGESELDALLGQLKARQTALADRHGRYVPIALKIAPD
                     LDDAQILNIADALRRHRIDAVIATNTTLARDKVQGVPYGDQQGGLSGAPLFEASTAVV
                     AALARALQGELPIIAAGGIFDGAQALAKLQAGAQLVQVYSGLIYRGPALVRECVAATR
                     DAA"
     gene            complement(8425..8910)
                     /locus_tag="PLH85_04330"
     CDS             complement(8425..8910)
                     /locus_tag="PLH85_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63815.1"
                     /translation="MPHLPSAFVAATLLACAPALHAGDSLDTLADAFANAPHRDWLSV
                     SGASHHFQPHRRAWREFNPGVGFERSFNGTPWVASAGYFRNSYDRDTWYLGGRWMPLA
                     AGPFRFGAFGLLASGYPSPVLVLPAVSVEAGRVGANLVALPNLPGYSGYLGLQVRVAL
                     D"
     gene            complement(8918..9658)
                     /locus_tag="PLH85_04335"
     CDS             complement(8918..9658)
                     /locus_tag="PLH85_04335"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNB63816.1"
                     /translation="MLKDYPYSQLQFYATAPYACSYLSERQARSQVATPSHLIDAELY
                     SELVRSGFRRSGIFTYRPYCDNCRACVPVRIPVSGFRPTRSQRRAWKSHALLDPHELP
                     LEFDEEHYALYQRYQATRHAGGGMDQDNREQYAHFLLQSHVDSRLVEFRENGVLRMVC
                     VIDCLTDGLSSVYTFYDPDPSCAGLGTFGILWQIEVCRQLGLPYLYLGYWIRDSRKMA
                     YKARFRPIQGRVGGQWQILSDADLDLPE"
     gene            complement(9663..10367)
                     /gene="aat"
                     /locus_tag="PLH85_04340"
     CDS             complement(9663..10367)
                     /gene="aat"
                     /locus_tag="PLH85_04340"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNB63817.1"
                     /translation="MIPWLGDHPAFPPVRRALRHPNGLLAAGGELTPPWLLEAYRHGI
                     FPWFNDGEPILWWSPDPRMVVFPSEARITRSLRKTLRKGAFEVRLDHDFSAVIRACAA
                     PREPDGRTWITPEIQAAYGRMHALGYAHSVETWIDGELAGGLYGMALGHVFFGESMFS
                     RRTDASKIAFAHLIRFLEQNDFRVLDCQMTTAHLASLGGREIPRAEFARLVDTHQRTG
                     PPPGRWPQDGAQAPWT"
     gene            complement(10413..10967)
                     /locus_tag="PLH85_04345"
     CDS             complement(10413..10967)
                     /locus_tag="PLH85_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB63818.1"
                     /translation="MPSKKTITGFFVAIAFSGAVFAAEPPAAADESQVSAFFERYSDS
                     LRAYTDSVKALMDRSLNFLGVPYRLGGTNPLTGFDCSGFVGKVFTDALGFGMPRTAAE
                     MAQMGEQVNRSDLKPGDLVFFNTMRRAFSHVGIYLGNNQFVHAPSAGGVVRVEDMRIA
                     YWAARYDGARRLLPSAQARQSQAN"
     gene            complement(11257..12087)
                     /gene="serB"
                     /locus_tag="PLH85_04350"
     CDS             complement(11257..12087)
                     /gene="serB"
                     /locus_tag="PLH85_04350"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNB63819.1"
                     /translation="MNLVVQGPKLEARALKDLSTLAGSQGLGRLGTNAFRLLDAHLTP
                     EVSAYCEAHGLDCAEVPEGRRLQDYRLFVTDMDSTLITIECIDEIADMHGLKAEVSAI
                     TEAAMRGELDFRASLSRRVALLEGLPEAALARVYQERLHLNPGAEALMTGLKRAGVVT
                     VLVSGGFTYFTDRLKARLGFDYAFANQLEVRSGRLTGRVKGEIIDGDAKRRALRLVRE
                     HHGFARNTVIAAGDGANDLAMLAEAGIGVAYHAKPVVRAQATHALSHAGLDGILHLFN
                     "
     gene            complement(12087..13160)
                     /locus_tag="PLH85_04355"
     CDS             complement(12087..13160)
                     /locus_tag="PLH85_04355"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNB63820.1"
                     /translation="MSSAQTDNLNIAAFDRMPSPDEITTRVPLSPAAEQTVLTGRRTL
                     QAILDHQDPRLFVVVGPCSIHDPVAGIDYARRLKTLADEVADTLVLVMRVYFEKPRTA
                     TGWKGYINDPYMDDSFRIEEGMEKARSFLLQVNQVGLPAATEALDPIAPQYYGDLISW
                     TAIGARTSESQTHREMASGLSTPVGFKNGTDGSLDAAVNGIISAANPHSFLGINGQGQ
                     SSIIRTRGNRYGHVVLRGGGGRPNYDTVSVSLAEKALAKAKIPANIVVDCSHANSWKN
                     PEYQPLVMRDVAHQIREGNRSIVGLMIESFIEAGNQPIPADLSTLRYGCSVTDACVSW
                     DTTVDMIRNTRDILRDVLPQRGA"
     gene            complement(13235..13876)
                     /locus_tag="PLH85_04360"
     CDS             complement(13235..13876)
                     /locus_tag="PLH85_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNB63821.1"
                     /translation="MKPVWLFDLDNTLHNASPHIFPHINRSMTAYLMRHLALDEAAAN
                     RLRVDYWRRYGATLTGLMRHHGTNPHHFLHDTHQFPELHRMVVADAALSHLLRRLPGR
                     KIVFSNGPRRYAEAVLDVLGVRHHFSDVCAVEQMRFHPKPDIRGFRHLLRQQRLQPAR
                     CILVEDSADNLRTARRLGMKTVWISRSLRRPAWVDVRLPSVLALARHPLRRGR"
     gene            complement(13893..14792)
                     /locus_tag="PLH85_04365"
     CDS             complement(13893..14792)
                     /locus_tag="PLH85_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB63822.1"
                     /translation="MTAPDFQLDLRLVRRRAGRAAATYAEVDALAREVARRMGERLDY
                     IRIAPSRILDLGCGPGADLPAFAARYPDVPRLAVDVARPMLALARDAHGPQQGFFQRL
                     LGSRGTPSAPGFLCADARALPLKRGAVSLVWSNLMLQWLHDPLPALKEMHRVLEVGGM
                     LMFSTLGPDTLRELRSVLPGPDGARLHRFIDMHDLGDALVQAGFSDPVMDMEMLTVTY
                     ASLDDLLRDLRLSGSACASAGRPRGLSGKGHWSGLRAAYEALRRNGRLPATVEVVYGH
                     AWKVAPKVAEDGRAIVRFERPPR"
     gene            complement(14806..15795)
                     /gene="bioB"
                     /locus_tag="PLH85_04370"
     CDS             complement(14806..15795)
                     /gene="bioB"
                     /locus_tag="PLH85_04370"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNB63823.1"
                     /translation="MTATVAPHEPLTTAPPSTPRKRWSVAEVDALFALPFNDLIFRAQ
                     QVHRAHFDANAVQRSTLLSIKTGGCSEDCGYCSQSARYDTGLEKETLLPLGEVLDNAR
                     AAKAKGATRFCMGAAWRGPKEKDLAPVLDMVREVKAMGLETCVTLGMLKDGQAEQLKD
                     AGLDYYNHNIDTAPEFYGQIITSHTLQDRLDTLDKVRKAGINVCCGGILGLGETRKSR
                     AAFIAELANMDPPPDSVPINNLVQIEGTPLGNAEAIDPFDFIRVVAAARITMPESYVR
                     LSAGRQELGDGFQALAFLAGANSIFYGERLLTTDNPDADADDRLFARLGLKSA"
     gene            15912..16568
                     /locus_tag="PLH85_04375"
     CDS             15912..16568
                     /locus_tag="PLH85_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNB63824.1"
                     /translation="MLPQACFACGAPSGDGVLCDACYGALPGRDCPRCPRCALPQPGG
                     GLCGACLRETPPFEATRVALDYAFPADSLVLALKYAHRLATTRLFVDLLAAAPRPEAD
                     LVVPMPLHPARLAQRGFNQAAELARPLARHWQLPVRLDGVVRDVNTVPQAGLPWKARA
                     ANVRGAFRVTGRLDGRRVVVVDDVMTTGATLAELARALKAAGAVSVENRVLARTPPPA
                     "
     gene            16602..17066
                     /locus_tag="PLH85_04380"
     CDS             16602..17066
                     /locus_tag="PLH85_04380"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNB63825.1"
                     /translation="MLHVVLHQPEIPPNTGNIMRMCANSGARLHLVKPLGFTLEEKQL
                     VRAGLDYRDLAVVTVHEDWPACRAALAGRRCFALTSHGGRHYGDVAFADEDVFLFGAE
                     SRGLPAELHAAFDDEHRLRIPMRPGNRSLNLSNAAAVVVYEAWRQLGFAGGI"
     gene            complement(17082..18062)
                     /locus_tag="PLH85_04385"
     CDS             complement(17082..18062)
                     /locus_tag="PLH85_04385"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNB63826.1"
                     /translation="MHIGVFGAGAWGTALAIAFSRQHRVTLWSREAREIADLRSDGEN
                     RRYLPGIPLPAGLHPSDSLAEAARADLHLVVTPLAGLRATARALHDLAPGTPLLWACK
                     GLEAGTAQLPHQIVADELGGDAPCGVLTGPSFAAEVAKGLPAAVTLAARDLAFARHWV
                     NALHDPRLRLYANDDLVGAEVGGAVKNVMAIAAGVADGMGFGLNARAALITRGLAEIT
                     RLGIALGGRRETFMGLAGLGDLVLTCTGDLSRNRRVGLLLAEGKTLPDILATLGHVAE
                     GVSTAREVAALATRLGVDMPITAAVDGVLHGGLGAREAVEQLLARDPKQE"
     gene            complement(18062..18514)
                     /locus_tag="PLH85_04390"
     CDS             complement(18062..18514)
                     /locus_tag="PLH85_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB63827.1"
                     /translation="MPLRRLTVAALLALPCALAATPASASDFRSLAEPAILYDAPSKQ
                     GKPLFVIQRYTPVEMVVSVDRWVKVREPAGSLLWLERRQLSEKRTLIVTAARAEIRQK
                     PDGNAPLAFEAAKDLVLELADAPADGWVKVRHRDGSTGFIRMTQVWGL"
     gene            complement(18514..18972)
                     /gene="secB"
                     /locus_tag="PLH85_04395"
     CDS             complement(18514..18972)
                     /gene="secB"
                     /locus_tag="PLH85_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNB63828.1"
                     /translation="MSETAQQPVFSIEKLYVKDLSVEVPNAPQIFLEREAPQIGVQLR
                     TESTAVDDGVFEVNLTVTVTAKLPDDKTVFLVEVAQAGIFQIRNVPQEDLEPLMMIGC
                     ANILFPYAREAISDAVSRAGFQPVILAPVNFEALFQQSRQASAGTDIPVQ"
     gene            complement(19023..19292)
                     /gene="grxC"
                     /locus_tag="PLH85_04400"
     CDS             complement(19023..19292)
                     /gene="grxC"
                     /locus_tag="PLH85_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNB63829.1"
                     /translation="MQPHILMYATGVCPYCVRAEGLLRRKGVTEIEKVRIDLDPVRRD
                     EMMSRTGRRTVPQIYIGDFHVGGCDDLFELDHQGRLDPLLRGEGA"
     gene            complement(19299..19724)
                     /locus_tag="PLH85_04405"
     CDS             complement(19299..19724)
                     /locus_tag="PLH85_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB63830.1"
                     /translation="MEFLQQNWYWALLAAVSGSLLVATSLQRGGQGISPAQATQLMNR
                     EDALVIDVRDAGEFAAGHVPNARHIPLADLEKRLGELESFKDKPVIVNCQSGGRSVNA
                     CNVLTKAGFTRVHNLDGGIQAWEQANMPLGRSGKSKKKA"
     gene            complement(19768..20091)
                     /locus_tag="PLH85_04410"
     CDS             complement(19768..20091)
                     /locus_tag="PLH85_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB63831.1"
                     /translation="MTSPIVDLIAKQEHIETAARALKAISHPLRLKILCVIGAEEACV
                     QEIVEAVGTSQSNISQHLAILRDKGVLLTRKDANRVYYRVGDTRTLQLIVMMREVFCG
                     DSAKG"
     gene            20339..21763
                     /locus_tag="PLH85_04415"
     CDS             20339..21763
                     /locus_tag="PLH85_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB63832.1"
                     /translation="MRSKLQQAGLVFTGLCAGILISLNFSANADKNSLPPLPVEELRN
                     LADVFNAIKQGYVEPVDDKKLITYAISGMLSNLDPHSTYLDPEAFKELQVGTQGEFGG
                     LGIEVGSEDGYVKVISPIEDTPAFRAGIKAGDLIIKLDDTPVKGMTLNDAVKRMRGKP
                     KTSIVLTIIRKGEAKPLTVTLVREIIKVQSVKSKLVEPGYGYVRVVQFQEQTAASLVQ
                     HLDRLVKQGELKGLILDLRNDPGGLLNGAVGVSAAFLPPKALVVSTDGRTDDARRKYF
                     AVPDDYLRGSRDDYIRNVPASIKTVPMVVLVNGGSASASEIVAGALQDHKRAVVMGTQ
                     SFGKGSVQTILPLNSNAAIKLTTARYFTPSGRSIQAKGIVPDIVVEETPNGSRERLRE
                     ADLDRHLVNAGEPDAPKSETPASRKDPAGKPRDKDNRDNKDKPKDEADEVKPAFEIAG
                     KDDYQLAQAVNLLKGLQILQNSAK"
     gene            21786..22241
                     /locus_tag="PLH85_04420"
     CDS             21786..22241
                     /locus_tag="PLH85_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63833.1"
                     /translation="MRRETAYKLAGRKHDHASHHAGSGLTKSREIRFKDLPPGQAVQA
                     RRSLAALKGVRVTDGKHPLELVVHYNVLDYTLELLEVALQNAGFHLDRPLLVRLHRAL
                     IYYVEETQVHNLRSPERLIKQSHEVYVKAYAAHPHGDHDDTPLELREYK"
     gene            22300..23049
                     /gene="moeB"
                     /locus_tag="PLH85_04425"
     CDS             22300..23049
                     /gene="moeB"
                     /locus_tag="PLH85_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNB63834.1"
                     /translation="MNDDQLLRYSRHILLPQIGIEGQSAIVSARVLVVGAGGLGSPAA
                     MYLAAAGVGTLVLADGDTVDLTNLQRQILHGEASVGRLKVDSGQETLSRLNPETRVES
                     LPRRLEGRELDEQVALADVVLDCSDNFATRHAINRACVAHRTPLVSGAAVRFDGQLSV
                     FDLRTDASPCYHCLFPEGEDVEEVRCAVMGVFAPLTGIVGTVQAAEALKLLAGCGETL
                     TGRLLLLDALEMDWRSVRLTRDPTCAVCASR"
     gene            complement(23077..24042)
                     /locus_tag="PLH85_04430"
     CDS             complement(23077..24042)
                     /locus_tag="PLH85_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB63835.1"
                     /translation="MIQSLTDAFIALQAWLFDALVQPVMFALGLGSFVELGFDGVEFV
                     LLGLLELAVAYLLLRPLEALWPAERWAARRAVRVDMLYTFLNRLGIVPLAVFLLLAPL
                     FAEVESWLRFHDLLPRQLEDLLPALDHHPLVSFLLYLVILDFAEYWRHRLSHVFRWWW
                     ALHGIHHSQRQMSFWTDSRNHLADDLIAGLWFAGLALFIGVPPGHFIGLLIALRLVEN
                     LSHANTRFDFGPIGDYLVVGPLYHRWHHALDLPAGPRYRHGCNFAILLPVWDVLFGTL
                     YLERRWPATGLHEASPEESNARDHFWAQQKLGFKLLWKALIGKPA"
     gene            complement(24039..24776)
                     /locus_tag="PLH85_04435"
     CDS             complement(24039..24776)
                     /locus_tag="PLH85_04435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB63836.1"
                     /translation="MKHLAALALTALVPAVAGAGKADCPAGTLYLTLDTGNMRHAEAI
                     ADILARHHIRATFFVAQEKTLRGDHALDASWAPFWKARVAEGHAFGSHTWRHGSFRKD
                     GADGRVSYRLMDGSTVQLDSAQVCEELRQPDRRFAELTGRPLDPLWRAPGGRTTPLTL
                     QAAEACGYRHVGWAPAGFLGDELPSDAFPNEVLVRRALDTLKDGDILMAHLGIWSRKD
                     PFAPMLDPLIGELQRKGFCFATIPERP"
     gene            complement(24773..25732)
                     /locus_tag="PLH85_04440"
     CDS             complement(24773..25732)
                     /locus_tag="PLH85_04440"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNB63837.1"
                     /translation="MRLPRFLLALTASVLPALACAETALVLNSADETVSFVDTVAYKE
                     TGRAPACKEPHHLMSTPDDSSVIVACAISNELVFFDPRNGKEQKRLHGISDPYQLGFS
                     PDNKWFVANSLRLDRVDLYTAADFKLVARVPAAKTPSHMAFDKASQFVFITLQDSNEV
                     LAIDLKTQQPLWKLPVGPTPAGIHMTPDDKYLLVGIMGADYVEVIDWRARKTVKRIVT
                     DKAAHNFLARGDGRHVFVSNRTVRGSISLVDMQTLSVVEKYDVPGGPDDMEIRADGKE
                     LWATARFARKVQVVDLEQKKLARSIPVGRSPHGVFFLNHAPRQ"
     gene            complement(25854..26942)
                     /gene="ribD"
                     /locus_tag="PLH85_04445"
     CDS             complement(25854..26942)
                     /gene="ribD"
                     /locus_tag="PLH85_04445"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNB63838.1"
                     /translation="MSASVDDTRWMARALQLAELGLFTTTPNPRVGCVLVRDGQVVGE
                     GWHRRAGEPHAEVHALAQAGDRARGATAYVTLEPCAHHGRTPPCADALVRAGVARVVA
                     AMEDPNPLVAGRGLARLREAGIATHCGVLETEARELNIGFVSRMTRGRPWLRLKAAAT
                     LDGKTALHNGVSQWITGPDARRDAHRWRARSCAVLTGIGTVRDDDPQLNVRAIPTERQ
                     PLRIVVDARLETPLHAHVLDGGPVLIAGAVDDPARIAALQARGADVLILPNDAGKVAL
                     PALLAELGRRGLNEITAESGFKLNGSLLREGCVDELVLYLAPTLVGDAARGLFNLPAL
                     DALADQRRLNFRDVRQIGPDLRIVARPA"
     gene            complement(26945..27424)
                     /gene="nrdR"
                     /locus_tag="PLH85_04450"
     CDS             complement(26945..27424)
                     /gene="nrdR"
                     /locus_tag="PLH85_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNB63839.1"
                     /translation="MRCPFCSAENTQVTDTRENEDGDIVRRRRRCLTCDKRFTTYERV
                     DLKMPQVVKRNGSRADFSRDKLLGSLSLALRKRPVMAESVEAAVDRIEAKLLSLGERE
                     VASELIGELVMRELKKLDKVAYIRFASVYRAFEDVDEFSEVIREVSPRPRGRPRSTS"
     gene            complement(27448..28698)
                     /gene="glyA"
                     /locus_tag="PLH85_04455"
     CDS             complement(27448..28698)
                     /gene="glyA"
                     /locus_tag="PLH85_04455"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNB63840.1"
                     /translation="MFSKQNTLARVDPELWQTVEAENRRQEDHIELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEHVDVAEQLAIDRLKALFGADAANVQPNSGSQAN
                     QAVLMAFAKPGDTIMGMSLAEGGHLTHGMPLNLSGKWFNVVAYGLDDKEEIDYEAMEK
                     LAREAKPRIIVAGASAYALRIDWERFAKIAKEVGAIFWVDMAHYAGLIAAGFYPNPVP
                     HADVVTSTTHKTLRGPRGGVILMKAEHEKAINSAIFPGLQGGPLMHVIAAKAVAFKEA
                     ATPEFKNYQEQVIANARVMARVLGEERGLRIVSGRTESHVFLVDLRAKNITGKEAEAA
                     LGRAHITVNKNAIPKDPQKPFVTSGIRIGSPAMTTRGFTEIEAERVAHLIADVLDNPN
                     DEATLAAVRSKVAELCRKFPVYGA"
     gene            complement(28774..30807)
                     /locus_tag="PLH85_04460"
     CDS             complement(28774..30807)
                     /locus_tag="PLH85_04460"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB63841.1"
                     /translation="MSRLNRLSLVHQIALAAGLISLIVFGTLVTFTTYFAEKADLAKT
                     GDELGHQIQGVVRMLELNHAGAVARAGSSLGRVQRAFGPLRIGPDTMASGSYQVPVVR
                     SGERILNGNKALLETLRDQIDADPAVLLRVGDEFVRAATLLRDKDGKSTEGTPLPRDG
                     KETRALLEGKSYRGVVRRNGTFFISAIDPMRDADGKVVGALAARVDIQSDMARLFAVL
                     GETRSGATGYTYVIAPGADAATSEVVFHPTLSGKKLNELNNPVLSRVVGEQIRLGKGT
                     LTYDWPANGGGEKTKMVAFDTAPSWGWIVATGSFVDEYTVHARELLRVLILLCTAGAA
                     LLVAVLYLIARSRIAPIRELVAVTERIGRGELNVAFPAVPDPTRNEVELITRALDSTT
                     RQMAGLIGGVTHTANAIKGAAHSLRADSESIVTSTARQSESASGLAAAVEELSVSITH
                     VSDSAALAREMTQDASAHADDGQHRMREMIDGMGRIAADMDTASAAVNSLSQRSARIS
                     NIGRIINDIADQTNLLALNAAIEAARAGESGRGFAVVADEVRKLAERTASSTREISET
                     VTLVQDEAQRVVDTIQGITTGVQNGVAVATASGAMLETIRSESARTTGAVNDIADATL
                     EQSSASQEVARNVEQIARMAEQNNQIIRDSHAQTTQLEQLADELEARVSHFRL"
     gene            complement(30898..31536)
                     /locus_tag="PLH85_04465"
     CDS             complement(30898..31536)
                     /locus_tag="PLH85_04465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNB63842.1"
                     /translation="MITRICLVRHGETAWNAERRLQGHTDIPLNDTGLAQARATAASL
                     AAEGFDAAYSSDLLRARQTAEAIAQRCALAITHDASLRERHYGHFQALTYDEARARFP
                     DDYHRFEGRHPDFVFPGGGESLNGFAARIRAALERIAATHPGRKVLVVTHGGVLDVAH
                     RLATGKPLDTPRDFTIPNAALNWLGWDEGRWSLIEWARQSHLHGALDELPNS"
     gene            31712..32302
                     /locus_tag="PLH85_04470"
     CDS             31712..32302
                     /locus_tag="PLH85_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNB63843.1"
                     /translation="MQALLKLSRGIDALTDRVGRTTTWLVLVVTLISAINAFVRYAVN
                     YSSNGLLEIQWYLFSAIFLLCAGYTLMRNEHVRIDVVTGHLSPRAHAWIDIVGTLFFL
                     APMALLVLYLSWPIFVNSFLTHEQSSNAGGLTVWPVRLLVPVGFALLILQAASEFIKR
                     VAFLKGLIDDPSAKHKGPSAEEGLAEAIRRQRGEEA"
     gene            32303..33853
                     /locus_tag="PLH85_04475"
     CDS             32303..33853
                     /locus_tag="PLH85_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNB63844.1"
                     /translation="MEFLAANMAPLMFGSLVIFLLFGYPVAFALAANGIVFGLIGIEL
                     GLLSPALFQALPDRIFGVMSNDTLLAIPFFTFMGLILERSGMAEDLLDTIGQLFGPVR
                     GGLAFAVIFVGAILAATTGVVAASVISMGLISLPIMMRYGYDQRLATGVIAASGTLAQ
                     IIPPSLVLIIMADQLGRSVGDMYQGAMIPGLVLTGFYVAYVALVAVFRPAAAPALPPE
                     ARSLRGMTLAIRVLTTLVPPLMLIFLVLGTIFLGVATPTEGGAMGAFGALVMAVLRRR
                     LNLSLLRQAMETTAKLTTFVVFILVGARVFSLTFYGVEGHLWVEHLLSGLPGGRLGFL
                     IVVNILVFLLAFFLDFFELSFIVVPLLAPVAEKLGIDLIWFGVLLGVNMQTSFMHPPF
                     GFALFYLRSVAPPSIKTTQIYWGAVPFVLIQIIMVGVIITFPDLVTMGLDSQEKVDAD
                     AIEIVVPQGDAPDDAPVQFDAGKDGDAPHAGDGQAGAGEQKDGIPVPPADDDATRALE
                     KALREDRR"
     gene            34354..36432
                     /locus_tag="PLH85_04480"
     CDS             34354..36432
                     /locus_tag="PLH85_04480"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63845.1"
                     /translation="MALLTSLSPDVFLERLRAALAEPRRDHPELARLARRLAAFIAAE
                     DLPDRLDAWHALMTWLAGGRLWQDVYIDVVDVHGPATQRLAVLMDVLAGDAELAGALR
                     AALRGLLDETDGANLFGEVGIPSDRGFLSEFGDRLTAKLLPQPRVDHDLAHLVGRSYR
                     RRRDLDLVRALHPSTLQRLMELLFPADGPEMTRALRRAMADGFRLLLARVAGQGLSEK
                     LRARGSAQAVADVPFARLARFGEALVADWEGGALPDAAMDAWQALSHACRAEMQVVSR
                     RLESEGISVDIVYGLDVLERCLNRLDALVAVMVEGGAAPVLALVQQLVLAAHQSSSLR
                     HLVRINLQRLQRRIVDRAGQTGEHYIADTPGEYRHIWTAAAGGGLLTVGTAAMKMTIA
                     GAGLALFLEGLGSGLNYAVSFLLLQAFGLILATKQPAMTAAAFAAIIRDRQGAERLDS
                     IVDAAARICHSQLAATIANVVVVAFGAYAFNGLWAWITGHAFLTASEARYVFKALSPV
                     DSGTVFYAALTGVILYLAALVGGWVDNWAVYHRLPEAIAEHRLGQRVGRLRMLRLAGI
                     VSRNMAGWATNVSLGLMLGMTPVLGRFFGLPLDVRHVTLNSGILSLAAAGMDLQWIGT
                     GHLSLAIAGVAVMFVLNLGVSFALSLLTAIRAYELPAEDLRELRRRLWQRLRTRPQDF
                     LMPRRLPPAP"
     gene            36565..37191
                     /locus_tag="PLH85_04485"
     CDS             36565..37191
                     /locus_tag="PLH85_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNB63846.1"
                     /translation="MNAPERVPFAGSRAVAGELACIVRVNEEIKAVVATATRINLMAL
                     NAIFLAKRAGNAALGFGVLSNELRRFAQDLTRHMAVLRDMTSGSVGSVTGVVQHQRNS
                     RILSEAIRLSGEGVPGVDMARRRCNQRLARELASLRGLDQRLRAAVEETQQLVELGGV
                     LARSAKIEAAYGGGFSPALMQVSTDFADVIAQIKRSLENLSKERLVKD"
     gene            37197..37937
                     /locus_tag="PLH85_04490"
     CDS             37197..37937
                     /locus_tag="PLH85_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB63847.1"
                     /translation="MKQAHYIYQDGDHRWLAIARDPNKPGYVIDTNEYVVADEEAALL
                     LDPGGIEIFPAVFSALSGECDPTRLMGIFASHQDPDIISSLALWLEFKPDLKCYTSWL
                     WSSFLPHFGGASETFVSIPDGGMTIKVGSLDLQAVPAHYLHSSGNFHLYDSKAKILFS
                     GDVGAALLPPDNNGLYVEDFSRHIRHAEAFHKRWMGSDRAKRDWCERVSRLEIDMLVP
                     QHGAIYQGPDVQRFINWFAALEVGVLRP"
     gene            38099..38488
                     /locus_tag="PLH85_04495"
     CDS             38099..38488
                     /locus_tag="PLH85_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNB63848.1"
                     /translation="MYLAIKHLHITCVALSLAGFLLRGLWMCADSPLLYASLTRRLPH
                     IVDALLLGSAIALAVMSGQAPFVQAWLTAKVAGLLVYIVLGALALRPGRPKPVRLGAF
                     GAALVSFSYIVSVALTKNPAGWLAMWG"
     gene            complement(38492..39232)
                     /gene="fnr"
                     /locus_tag="PLH85_04500"
     CDS             complement(38492..39232)
                     /gene="fnr"
                     /locus_tag="PLH85_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNB63849.1"
                     /translation="MHTKPVIPLSVTEVKIACSQCNLRELCLPFGLDPNEIDRLDELV
                     GSRRKIKRHQHLYRSGDPFEAIYAIRTGSFKTDVILEDGRDQVTGFQMTGEVMGLDGI
                     SIEHHTCNAIALEDSEVCVIPFGRLEELSREVESLQHQFHKIMSREIVRDHGVMMLLG
                     SMRAEERLAAFLLNMSQRFTARGFSPSEFHLRMTRDEIGSYLGLKLETVSRAFSRFQD
                     EGFVSVQQKHIRILDIAGLKALLNHGPT"
     gene            39355..40749
                     /gene="hemN"
                     /locus_tag="PLH85_04505"
     CDS             39355..40749
                     /gene="hemN"
                     /locus_tag="PLH85_04505"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNB63850.1"
                     /translation="MNTIHKDLIFDPQLIRKFDVNGPRYTSYPTADRFVEAFNSEALC
                     DWLGKRTIGGVGKPLSLYFHIPFCNTICYYCACNKIITKDHGRSAKYLRYVAREMEMQ
                     VAHLTGPREVLQLHLGGGTPTFLSHDEMRELMANVRKHFTLVPDGEFSIEVDPRKVDE
                     ATVALLAELGFNRMSVGVQDFNPDVQRAVNRVQSEEETRLVIESARAHGFKSVSVDLI
                     YGLPKQNVISFNRTLEQILALSPDRLSIYNYAHLPGLFKPQRRIHDAELPNAEAKLQI
                     LQLAIRRLTEAGYVYIGMDHFAKPDDELAVAQRQGRLHRNFQGYSTYAECDLLAFGVS
                     AIGKVGPTYAQNVKTLDEYYDIIDAGRLPVLRGIELTPDDLLRRSIIQALMCHFELSI
                     ESIEIAHLIDFPRYFAAELADLREMEKNGLVQIDDKWISVQPAGRMLVRAIAMVFDRY
                     LRADRERTRYSKVI"
     gene            40898..41605
                     /locus_tag="PLH85_04510"
     CDS             40898..41605
                     /locus_tag="PLH85_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB63851.1"
                     /translation="MPETGYLAVFLIGLLGGTHCVGMCGGIVSALTVRMPGEQRRDWP
                     LHVAYNFGRIASYTAAGAALGALGSAGMLLDGMLPVQLGLYVAANLMLVALGLYLTGF
                     TRALAGVERIGQRLWTRIQPLTRRFLPARSVAQALPLGMLWGFLPCGMVYSVLATALV
                     TGSAGRGALLMLSFGLGTLPNLLLAGLLLRHLREVVRNRSVRLVSGLVVLGFGVYGLL
                     NTSSLGNKLWNGVVCHV"
     gene            41636..42061
                     /locus_tag="PLH85_04515"
     CDS             41636..42061
                     /locus_tag="PLH85_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB63852.1"
                     /translation="MKILLAVDGSPVSLRAAEEVVRLARALKSEPDVHVVYVHLPIPL
                     DFATRHVGREAVEAYYRDEGEAVLQAPLAALTASDLMPQRHLHVGQPADVILQLAGEL
                     GCDLICLGRHGRGAMADLLLGSVANRVVQRAPMPVLLVG"
     gene            42071..44470
                     /locus_tag="PLH85_04520"
     CDS             42071..44470
                     /locus_tag="PLH85_04520"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNB63853.1"
                     /translation="MSDGPLPESMDELSITGMSCAACAVRLEKVLNRLPGVSAHVNFA
                     TGRASVRRATGAADGEALVAAVRKAGFDVAPETLELALSGMSCAACAARIEKVLNRLP
                     GVEATVNFNAETARVRYVPGLAGPDAMRAAVAKAGFSAEPVLARDREAERARKAEEAR
                     SEWRQFLLALVLSAPFLLQMPFMLAGGLDGHHEWLPRWLQGLLAAPVQFWVGRRFYRG
                     AWSSLRGGGANMDVLVALGTSVAFFFSLAVVLLGRHDQHVYFEASASIITLVLLGKLL
                     EARARARTSAAIEALLRLQPPTAWLERDGALVEVPVAQVARGTVFVLRAGDAVPVDGE
                     VLAGESSIDEAMLTGESVPVPKAAGDTVFAATLNGAGLLRCRATGVGAETLLAGIVRL
                     VEQAQGGKPAVQRLADRIAAVFVPTVVGLALLTFAAWWWAAGDPVQALVNGVAVLVIA
                     CPCALGLATPTAIMVGTGQGARAGILVRKVEALELAESLRIVAVDKTGTLTEGRPALV
                     ETRLAKGIAYNEALHLAASLEQGSPHPLAVALVAAAREAGIGLALPVAVQSAGGKGVS
                     GEVAGRQVAVGSPAYLTEAGVAIPADQLDPLTTGGLTPVCVAADGRLLALFGVADRLR
                     PGAAEAVARLRRQGLRVVMLTGDHPAVAQAIARQAGIDEFRAQVLPADKAAAVQALRN
                     EGRVGMVGDGINDAPALAAADVSFAIGAGSDVAVEAADVTLVRSDPAGVADAIALSRA
                     TLGKIRQNLFFAFIYNILGIPAAALGLLNPVIAAAAMAMSSVSVVSNSLLLRRWRPGQ
                     D"
     gene            44489..44695
                     /locus_tag="PLH85_04525"
     CDS             44489..44695
                     /locus_tag="PLH85_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNB63854.1"
                     /translation="MQEAIIQVGGMSCQGCVRNVTGALSAVAGVTAVEVSLERGEARV
                     NFDPARTGVAALRAAVEEAGFDAA"
     gene            44779..45306
                     /gene="ppa"
                     /locus_tag="PLH85_04530"
     CDS             44779..45306
                     /gene="ppa"
                     /locus_tag="PLH85_04530"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNB63855.1"
                     /translation="MGYDQVKAGKDVPNDINVIIEISAQGDPIKFEVDKDSGAVFVDR
                     FMGTTMRYPINYGYVPHTVAGDGDPVDVLVVTPFPLQPGVVIRARPVGVLKMEDEGGV
                     DAKVVAVPVSKLTPLYDKVQSVEDLPELLMKQTVHFFEHYKDLEPGKWVKVTGWGTVE
                     DARQEILNGIAAAAK"
     gene            45578..46006
                     /locus_tag="PLH85_04535"
     CDS             45578..46006
                     /locus_tag="PLH85_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB63856.1"
                     /translation="MTKQKQSGFTLIELMIVVAIIGILAAIAVPQYQSYTKKAKFTEV
                     VNATSSRKSFVEVCLQDPNIVAANCVGGSNGIPTDDTTGFNNVTSVTVTSGGTITATG
                     KAAAFGSSGNIYQMTATVTNGNVVWDDKGSGSTCKTAGLC"
     gene            46166..>46431
                     /locus_tag="PLH85_04540"
     CDS             46166..>46431
                     /locus_tag="PLH85_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63857.1"
                     /translation="MPLEQKDMCEIARRALRLPVFSWLVPVGFMLLFLAIALGSISGL
                     IAPLGPWLNDEFRPHAYPAVGILVLTVYAFSGPSWRELGLLVFSG"
BASE COUNT         7118 a        13821 c        17325 g         8067 t
ORIGIN      
        1 gtcactcgcc ccgcatgcgc tgggcgacgg cctcggccac cgcgtgttcg ccggcgtccc
       61 acaggccgtc caccacgccc tggcggtcgg cggggctgcg gttctggctc atcttccact
      121 tgccttcgag acgctggacg gggatttcga ggccgacgat ggccttgagc tgggcgctga
      181 tgtagtcggc gggggcgtcg tccacgcgcc agggggtggc gcggggggct tcctgatggt
      241 cggtgaggtc ggtgacgagg cgcagcagcc aggcggggtc gtcgatgagg cgggcggggc
      301 cccaggcgtg gacggtggcg aagttccagg tggggacgac cttgccggtg gtgcgcttgc
      361 tggggtacca ggcgggggtg atgtaggcct cggggccgcg gaagacgacg aggcagtcgg
      421 gctgggcggc gagctcggcg agctgggggt tggggcgggc caggtgggcg cgcagggtgc
      481 cgtgggggcc ttcgtccggg tagaagagga cgggcacgag gttggcctgc aggccgccgg
      541 gcccctggct gatgagggtg gcgagggggt gggcgttaat cagctcggcg cagcgggcgg
      601 ggtcgtcttc gcggaacagg ggcggcaggt acatggcggg tctcgggcgg gagaggcggt
      661 gttgcgcggc cagtgtagca ggccgggcgg ggtggccgtc agctgatgcc gccgcgcttg
      721 cgcacgcagt ccacgtaggc gcggccgtcc aggggggcct tgtcgcgctg ggcgcgccag
      781 agggtttcgc cgaggcattc gaggacgtcg tggagggcgg cgtggcggtc gccgtggcgg
      841 gcctggcact gctcgaaggc gtcgcggatg ccggggggct ggttgatgga gagctgctcc
      901 tcgatggcca ggtgcaggga gaggtgcagg aaggggttga tctggccgtc ctcggggctg
      961 aagtccttgt gcacggcgtc cgcgtcttcc agcaggggat ggtattccgg gtggtgggcg
     1021 acgaggtcgg cggcgagggt ctcgagctgg gtgaggacgt cccggttgcg gtatttgcgc
     1081 caggcgtcga ggaagaactg gcgggcctgg tcgcggctgg ggtcgaacat ggggggnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg cctggtcgcg gctggggtcg
     1261 aacatggggg gctccgggaa gaaggcggaa gagggaggag gcgcgttcag gcggtcttgt
     1321 cggggaagcg gcagaggtcg cgcagcaggc attcgccgca cttgggcgtg cgggcgacac
     1381 agacgtagcg gccgtggagg atgagccagt ggtgggcgtc gcgcaggtag gccttgggaa
     1441 tgcggcgcag gaggcgcttt tccacttcga gcacgtcttt gccgggggcc aggccggtgc
     1501 ggttggcgag gcggaagatg tgggtgtcca cggccatggt ggggtcgccg aagacggtgt
     1561 tgaggaccac gttggcggtc ttgcggccga cgccggggag ggcttcgagg gcggcccggt
     1621 cgtgggggac ttcgccgccg tggcgttcca gcagttgctg cgacaggagc agggtgttgc
     1681 gggccttgtt gcggaacagg ccgatggtct tgatctgttc ggcgatggcg tcctcgccca
     1741 ggctcaccat ggcctccggc gtgggggcga gggcgaagag gcggcgggtg gcgagattca
     1801 cgcccttgtc ggtggcctgg gcggagagga ccacggccac caggagctgg taggggctgg
     1861 cgtattccag ctcagtggtg gggtgagggt tggcggtggc gaggcggcgg aaggcttctg
     1921 cgatctctgc caggcgcatc tcggccatgg gtcagccggc ttcgaccggg gtgtcgatgg
     1981 acggcggggc ctggcgggcg cgggcggcct tgcgggcttc gagccagttg cggccggcga
     2041 cgaggctgcc cagggcgaag aaggcaccag gcgggaggat ggccaccagg aagccggggt
     2101 agtcgtcgcc gaagaggacg atgggcgaag cgccggggat gatcatctcg atgcccgaga
     2161 gcagggtgcc gctgccaacg aattcgcgca gggcgcccag gaccaccagc acgagggtga
     2221 ggccggcgcc ctgggcgatg ccgtccaggg tggagccgag gggctcgttc ttggcggcga
     2281 aggcttcgac gcgggcgagc acgatgcagt tggtgacgat gagcgggatg aagatgccga
     2341 gcacgaggta gaggtcgtgc acgaaggcgt tcatggagag atccaccacg gtggtgaggg
     2401 cggcgatgat caggatgaac accgggatgc ggatctcgta ggggatgaag ttgcgcagcg
     2461 ccgccacggc gaggttggac atgcccatca cgaacatggt ggcgagcccg aggctgaggg
     2521 cgttgacgat gttggtggtg atggcgaggg tggggcacat gcccaggatc tgcacgatgc
     2581 cggggttctg cttccaccag ccgttccagg cgatgtcgcg ataggccttg atgtccatgg
     2641 tcttcctccg ctcagagggg cttgccgctg ggggcggcaa agaggcggtt ctggttgtcg
     2701 gcggcaaagc gcgcggcgcg ggccaccgcg ttggtgacgg cgcgggcgct gatggtggcg
     2761 ccggtgtggt aggcgatctg gccaccgtcc ttgcgcacct tccattggtc cagcggggtg
     2821 ctggcggggt tcaggtcggt gaactggacg atccacgggg cggctttgtt cttgtccttc
     2881 ttgggatcga tgtagtcgcc caggccgggg gtttcccggt ggccggtgac gcgcacgcca
     2941 gagaggctgc cgtcggcgcg cacggcgacg atgaggttga ccttgccgct gtagccgtcc
     3001 ggggcgatgg cctccagcac cagcgcggcg ggctggccgg ccttgcgggc gcggtagatg
     3061 cggccgccgt catccaggcc cagggctggc gtggggcccg tcaggatgaa gtcgtcgagg
     3121 agggcgttgt cgtaggcggc agggggcagc acctcggcga tggacttcat cttctcgtcg
     3181 ttggccgagg cggcgatgcg ctcgcgggtg gcgtcttcgg tgaaggccat gagggcggtg
     3241 aataccaccg tgaaggcgat catcacggcg ccggtgcggg tggacaggcc gagggcggtg
     3301 gtttcgctca tggcgctcag tccttcttgt ggccgaagac cggggactgg gtcttcatgt
     3361 cgatgagggg gacgaggatg ttcatcagca gggtggcaaa ggcgatgccg tcggggtagg
     3421 cgccgaaggc ccggatgatg taggtgaggc aggcggcccc ggcggcgaag aggagcttgc
     3481 cgcggggcgt ggtggcgccg gagacggggt cggtgaggat gaagaaggcg cccagcatgc
     3541 tgccgcccga ggccaggtgg aagaggggcg aggcgaagcc ggcgggcttg acggcccaca
     3601 gcaggccggc cacggcgaag agggccgcgc agaaggcgaa gggaatgtgc caggtgatga
     3661 tgcgccgctg gatcagccac aggccgccga gcaggtagcc gagggcgacc cattcgctgc
     3721 cctggccgcc gagggtgcca aaggcggggc cgtccatgag ctgcgacgcc ggcaggttgt
     3781 agaggggcga atcgggattg gcggcagact tcagcgcggt gcgcagggcg tcgagggggg
     3841 tggcgccggt gatggcgtcc gcggcccggg cgccgccgaa gatcaggtcg agctgggtgg
     3901 tgaaatcgag ctggccgggg gcgggccact gggacatcag cgccgggtag gcgacgatca
     3961 tcaggcagaa ggcggccatg gccgggttga aggggttctg accgaggccg ccgtagagct
     4021 gcttgaccac cacgatggcg agcagggtgg cgagtacggt gatccaccag ggcacgatgg
     4081 gcgggaaggt gagggcgatc agccaggcgg tgacgacggc cgacaggtcg gtcaggaaca
     4141 gcgcccgcgg ccgcccgcgg acggtgagca gggcggcttc gccggccagg gcggcggcgg
     4201 tggcgagggc gaggttgacg aggatgccgg cgccgaagaa ccagacgtag gcggcgatgc
     4261 ccggcacgag ggcgagcagc acctggagca tgacgctctg gacgctggcg ggcttgcgga
     4321 tgaaggggga gttgagcatc gggttcggaa ggcgggaggt ccgtcaggcg ctgccggcgg
     4381 gcggttcgtc ggccttggcg gcggcgggcg cgtcggaagc cgcctggcgg cgggcgtcga
     4441 tggcgtcgat gtcggcctgc tggccgggcg agaggtgatc ggtgttcttg ggtgtgacct
     4501 gggccttctg ctggcgggcc ttttccatgg cggcctcgat gagggcgcgc ttggcgtcgt
     4561 caggactggg cttggcggcg ggcgcggtgc cggttgccgg ggcgtcgccc gccggtttgg
     4621 cggcggcctg ttcgcgggtg gcggcggcct tggcggcgag cttttcggct ttttcctgct
     4681 tttcccgttc ctggcggagg tttttgtatt cgaagcggtc gcgggcccgg tcggaagcgt
     4741 ccttgtcccg ctcccgtgcc cagatttcgc tcttggcaaa gcggtagtag tccaccagcg
     4801 ggatctgcga cgggcagacg tagctgcagc aaccgcattc gatgcagtcg aagaggtggt
     4861 attcctgggc cttgccgaag ttcttggagc gggagaacca gtacagctcg aagggcgcga
     4921 gatccgcggg gcaggccttg gaacattcgc cgcagcggat gcacggcatc tcgggcggcc
     4981 gcggcgggaa cagggcgggg ctgccgacga ggatgcagtt ggtggccttg accacgggca
     5041 cgtcgaagga cggcatgcgc aggcccatca tggggccacc catgatgtag cggtcggtgt
     5101 cggcgccggg gccggcgagg cgcaccacgt ggtccatagg ggtgcccagg agcacgtcgt
     5161 agttgcgcgg gtgggccaca ttgccggtga cggtgacgac gcgcgacacc agcgggcgag
     5221 cccggtcaag ggcttcgtgg atggcgaagg cggtgcccac gttgaagcac tgcacgccgt
     5281 agtcggtgga gcggcggccg tggggcacct cgatgccggt gaggacgcgg atgagctgct
     5341 tggcgccgcc ggccgggtag cgggtgggga tggccacggc ctcgatgccg gcctcgccgc
     5401 gggcggcaat ggcggcctgc acggcggcca cggcttcggg cttgttgtcc tcgatgccga
     5461 tcaggacttc ttcggcctgg agcatggcgc gcatggcgga gatgcccttg agcatgtcgc
     5521 cggcgcgctc acgcatgagc aggtcgtcac aggtgatgaa gggctcgcac tcggcgccgt
     5581 tgatgaccag ggtcttggtg tggccgactt tgccgggctt gagcttgaga tggctgggga
     5641 agactgcgcc gccgaggccg accacgccgg cgtcgcgcag gtagtcgcgc agctcgtccg
     5701 gagcggcaga cttgaggttg atcgtctcta gctcgcccca gcggtcctcg ccatccggtt
     5761 cgaggatcac cgacagggcg ctgaggccgg aggtgtgggg catcagggcc ggggccacgt
     5821 cgcgcacgag gcccgaggtg gaggcatgca ccgcggagga cacgttgcca tccgcagcac
     5881 cgatgcgctg ccccttgaag acgcgttcgc cgggcttgac cagtgggcgc ggctggccgc
     5941 ccacggcctg gtgcagcggg atcaccagcc ggtcgggcat gggcaccggg gcgatcggcg
     6001 cggtggtgga ttgggccttg tgggtctggg gcttgacgcc gccgtggaag ctgaagagtt
     6061 tgcgcagcat gtcaggcttt gttccgggcc gtccgaaggg ggctgaatag gtgtctcatc
     6121 aggccgcccg gtcggcgggg gcggcttcgg cgaggggaat caccgggtag cgccacttcc
     6181 aggtgtccac ggtctcggcg atggggcgca tgtggatgca atccaccggg cagggggcga
     6241 tgcacagttc gcagccggtg cattcgcggg cgaccacggt gtgcagctgc ttggccgcgc
     6301 cgacgatggc atccaccggg caggcctgga tgcacagggt gcagccgatg caggtgtgct
     6361 cgtcgatgac ggcgacggac ttgggttttt cgacgccatg ctcttcggag aggggcttga
     6421 actcgcggcc gaggagatcg gacagcttgc ggatgccttc ttcgccgccc ggtgggcact
     6481 ggttgatctc ggcctcgccg ttggcgatgg cggtggcgta gggccggcag ccggggaagc
     6541 cgcactggcc acattgggtc tggggcagga tcgcgtcgat ctggtccacc agcgggtccc
     6601 cttcgacctt gaacttgatc gaggcgaacc ccagcatcgc gcccaggacc agggcgatgg
     6661 cggtcatgac gaggagggcg gagagcatgg cggtgcgggt cagtggtact tgtcgaggcc
     6721 ggcgaagccc atgaaggcga ggctcatgag gccggccgtg acgaaggcga tggcggtgcc
     6781 cctgaagggc gcaggcacgt cggcgccatc gaggcgttcg cggatgccgg cgaacaggat
     6841 cagggcgagg gagaagccca ccgagctgcc gaaaccgaag agcagtgact ggagcaggtc
     6901 gtggtgcagg gccacgttga gcaggggcac gcccagcacg gcgcagttgg tggtgatgag
     6961 gggcaggtag atgcccagca cctggtgcag caccgggctg gtcttctgga tgatgagttc
     7021 ggtgagctgg acgatggcgg cgatgatgac gatgaaggcc agcgtctgca ggtagctcag
     7081 gccgaaggcg ctgagcacgt agtgatcgat gaggtagctg gtgccgcagc cgagggtgag
     7141 cacgaaggtg gtggcagcgc ccatgccgac ggcggtgtcg agcttcttgg agacgcccat
     7201 gaacggacac aggccgagaa tgcgcaccag gacgacgttg ttcacgagaa cggcgccgag
     7261 gatcacgaga aggtagctgg acatgggccg ggcttcagcg taggggcaaa ggcggattat
     7321 cccgcgacgc gggcgaaaga ctcaagtcgt tgaatcaaga cggacatcca aaccggattc
     7381 aggccgcgtc acgtgtggct gcgacgcact cgcgcacgag ggcggggccg cggtagatga
     7441 gcccgctata cacctggacg agctgtgcgc cggcctgcag cttggcaagc gcctgggcgc
     7501 cgtcgaagat gccaccggcg gcgatgatgg gcagctcgcc ctgcagggcc cgggcgaggg
     7561 ccgcgacgac ggcggtggag gcttcgaaca ggggtgcgcc ggagaggccg ccctgctggt
     7621 cgccgtaggg cacgccctgg accttgtcgc gggcgagggt ggtgttggtg gcgatcaccg
     7681 cgtcgatgcg gtggcggcgc agggcgtcgg caatgttgag gatctgggcg tcgtccagat
     7741 ccggcgcgat cttgagggcg atgggcacgt agcgcccgtg gcggtcggcg agggccgtct
     7801 ggcgtgcctt gagctggccg aggagggcgt cgagttcgga ttcgccctgg agctggcgca
     7861 ggttcttggt gttgggcgag gagatgttga cggtgacgta actggccagc gggtaggcct
     7921 tgtcgaggca ggcgaggtag tcgtcggcgg cccgttcgat gggcgtgtcg gcgttcttgc
     7981 cgatgttgat gccgaggatg ccccggtacc tggccgcctg gacgttggcc accagggcgt
     8041 cgatgccatg gttgttgaag cccatgcggt tgatgatgcc gcgcacctcg ggcaggcgga
     8101 acatgcgcgg cttgggattg ccggcctggg gccgcggggt gatggtgccg atctcgatga
     8161 agccaaagcc gaagcgggcc agtccgtcga tgacctcgcc gttcttgtcg aggccggcgg
     8221 ccatgccgac ccggttgggg aagcgcaggc ccatgacatc caccgggcgg gcgtcgaggg
     8281 gtttgccggc gggcagcaca cggccggcga cgttgagggc cgcgagggcg gcctcgtggg
     8341 cggtctcggc gtcgaaggag aacaggacgg ggcgggcgat atcgtagagc atccgccatt
     8401 ttaccgcagt gcggcagggc ggtgtcagtc gagggcgacg cgcacctgca ggccgagata
     8461 gccgctgtag cccggcaggt tgggcagcgc caccagattg gcgccgacgc ggccggcttc
     8521 aacggacacg gcgggcagca ccagcaccgg tgaggggtag ccgctggcca gcaggccgaa
     8581 ggcgccgaag cggaagggac cggcggcaag gggcatccag cggccgccca ggtaccaggt
     8641 gtcccggtcg tagctgttgc ggaagtagcc ggccgaggcc acccacgggg tgccgttgaa
     8701 gctccgttcg aagccgacac ccggattgaa ttcacgccac gcccggcggt ggggctggaa
     8761 gtggtgggag gcgccggaga cgctgagcca gtcccggtgc ggggcgttgg cgaatgcgtc
     8821 ggcgagtgtg tcgaggctgt cgccggcatg cagggcgggc gcacaggcga ggagggtcgc
     8881 ggcgacgaac gccgacggca ggtggggcat gggggattta ctccggaaga tcgaggtcgg
     8941 cgtccgagag gatctgccac tggccgccca cacgcccctg gatggggcgg aagcgtgcct
     9001 tgtaggccat cttgcggctg tcgcggatcc agtagccgag gtagagatag ggcagtccga
     9061 gctggcggca gacttcgatc tgccacagga tgccgaaggt gccgagcccg gcgcaggacg
     9121 ggtcggggtc gtagaaggtg tagacgctgg acaggccgtc ggtcaggcag tcgatgacgc
     9181 acaccatgcg cagcacgccg ttctcgcgga attccacgag gcggctgtcc acgtggcttt
     9241 gcagcaggaa gtgggcgtac tgctcccggt tgtcctggtc catgcccccg ccggcgtggc
     9301 gggtggcctg gtagcgctgg tacagggcgt agtgttcctc gtcgaattcc agcgggagct
     9361 cgtgggggtc gaggagggcg tgggatttcc aggcacggcg ctggctgcgg gtggggcgga
     9421 agccgctcac cgggatgcgc accgggacgc aggcacgaca gttgtcgcag taggggcggt
     9481 aggtgaagat gccgctgcgg cggaagcccg agcggacgag ctcgctgtag agctcggcat
     9541 cgatgagatg actcggggtg gccacctggg agcgggcctg gcgctcggac aggtaggaac
     9601 aggcgtaggg cgccgtggcg tagaactgga gctgggagta gggatagtcc ttgagcatcg
     9661 ggctacgtcc agggggcttg ggctccgtcc tgcggccagc gaccgggcgg cgggccggta
     9721 cgctggtggg tgtcgaccag acgggcaaat tccgcacggg gaatctcccg gccgcccagc
     9781 gaagccagat gggccgtcgt catttgacaa tctagcacgc gaaaatcgtt ctgctcaagg
     9841 aagcggatca ggtgcgcgaa ggcgatcttg gaggcgtccg tacggcggct gaacatggat
     9901 tcgccgaaga acacgtggcc cagcgccatg ccgtagaggc cgcctgccag ctcgccgtcg
     9961 atccaggttt ccacgctgtg ggcgtagccg agggcgtgca tgcggccgta ggcggcctgg
    10021 atctccggcg tgatccatgt gcggccgtcg ggctcacggg gcgctgcaca cgcgcggatc
    10081 acggctgaaa agtcgtggtc gaggcggacc tcgaacgcgc ccttgcgcag ggtcttgcgc
    10141 agggagcggg tgatccgtgc ttcggatgga aacaccacca tgcgtgggtc gggactccac
    10201 cacaggatgg gttcgccgtc gttgaaccag gggaagatgc cgtgccggta ggcctccagg
    10261 agccagggag gtgtcagttc gcctcccgcg gccaacaagc cgttggggtg ccgcagtgca
    10321 cggcggacgg gcgggaatgc ggggtggtcg ccgagccagg ggatcatggc cgggagcgcg
    10381 gtgcaggggg tcgcctgagg ggcgggccgg ggtcagttgg cctgggattg tcgcgcctga
    10441 gcgctgggca gcaggcgccg ggcaccgtcg tagcgggctg cccagtaggc gatgcgcatg
    10501 tcctccacgc gcacgacccc gcccgcggac ggtgcatgga cgaactggtt gttgcccagg
    10561 tagatgccca cgtgggagaa ggcacggcgc atggtgttga agaacacgag gtcgccaggc
    10621 ttgaggtcgg agcgattcac ctgttcgccc atctgggcca tttcagcagc cgtgcgaggc
    10681 atgccgaacc ccagggcgtc ggtgaagacc ttgccaacga agccgctgca gtcgaagcca
    10741 gtgagggggt tggtgccacc caggcggtag ggaacaccga ggaagttgag gctgcggtcc
    10801 atcagcgcct tgaccgaatc ggtgtaggcg cgcagggagt cggagtagcg ttcgaagaac
    10861 gcgctcacct gggactcgtc ggcagctgca ggaggctcgg cggcgaagac cgcgccggag
    10921 aacgcgatcg ccacaaaaaa gccggtgatg gtttttttgg agggcatcgg gacactatac
    10981 cgaagggtgt ctggagtgta aagattttcg acaagccagg aatggcgcgg gttttgggtg
    11041 cagtgcacca tgtgagttgc gccacggacg aagcgtgatg tcatgggtag catgggtccc
    11101 gcactggtcg cgagcagcaa cggacagcac gccgcgcggg aggcggaccg aaccggtcgg
    11161 gatgtctccg tcaaagggcc cttcggcgcg ccgatccggg gcgctcgtgc cagtcgcacc
    11221 aatttgacga tctgttcggc cagtccctgg cgggactcag ttgaacaggt gaaggatgcc
    11281 gtccagcccg gcgtgactga gggcgtgggt cgcctgcgcg cggaccaccg gttttgcgtg
    11341 ataggcgacg ccgatgccgg cttcggcgag catggccaga tcattggcgc cgtcaccggc
    11401 ggcaatgacg gtgttccgcg cgaagccgtg gtgttcgcgc accagacgca gggcgcggcg
    11461 cttggcgtcg ccgtcgatga tctcgccctt gacgcggccc gtgagcctgc ccgagcggac
    11521 ttccagctgg ttggcgaagg cgtagtcgaa gcccaggcgc gccttgagcc ggtcggtgaa
    11581 gtaggtgaag ccgccggata ccagcaccgt gacgacgccg gcccgtttga ggccggtcat
    11641 cagcgcttcc gcgccgggat tgaggtgcag gcgctcctga tagacccgcg ccagggccgc
    11701 ctcgggcagg ccttcgagca gcgccacacg tcggctgagg gacgcgcgga aatccagttc
    11761 gccccgcatg gcggcttcgg tgatggcaga gacctcggcc ttgaggccgt gcatgtcggc
    11821 gatctcgtcg atgcactcga tggtgatgag cgtcgagtcc atgtccgtga cgaacagccg
    11881 gtagtcctga aggcggcgcc cttccggcac ttcggcgcag tcgagcccgt gggcctcgca
    11941 ataggcggac acctcgggcg tgaggtgggc gtcgaggagg cggaaggcgt tggtcccgag
    12001 gcgaccgagc ccctgggaac cggcgagcgt cgacagatcc ttgagggccc gcgcttcgag
    12061 tttcgggccc tggacaacca gattcatcag gcgccccgct ggggcagcac gtcccggagg
    12121 atgtcgcggg tgttgcggat catgtccacg gtggtgtccc agctcacgca ggcatcggtg
    12181 accgagcagc cgtagcgcag cgtggacagg tctgccggga tgggctggtt gccggcttcg
    12241 atgaagcttt cgatcatcag accgacgatg gagcggttgc cctcgcggat ctggtgggcg
    12301 acatcacgca tgaccagggg ctggtattcc ggattcttcc aggaattggc gtgggagcaa
    12361 tccaccacga tgttggccgg gatcttggcc ttggcgaggg ccttttccgc cagggagacg
    12421 gacacggtgt cgtagttggg acggccgccg ccgccgcgca gcaccacgtg gccgtagcgg
    12481 ttgccgcggg tccggatgat ggatgactgg ccctggccgt tgatgccgag gaaactgtgg
    12541 ggattggcgg cggagatgat gccgttcacg gccgcatcca gggaaccgtc ggtgccgttc
    12601 ttgaagccga ccggggtgga caggccggag gccatctcgc ggtgggtctg ggattcagag
    12661 gtgcgggcgc cgatggcggt ccagctgatg aggtcgccgt aatactgggg cgcgatggga
    12721 tcgagggcct cggtggcagc aggcaggccg acttggttga cctggagcag gaagctgcgg
    12781 gccttttcca tgccttcctc gatgcggaag gagtcgtcca tgtaggggtc gttgatgtag
    12841 cccttccagc cggtggcggt gcgcggcttt tcgaagtaca cacgcatcac cagcacgagg
    12901 gtgtcggcga cttcgtcggc gagggttttg agacgccggg cgtagtcgat gccggccacc
    12961 gggtcgtgga tggagcacgg tccgaccacc acgaacaggc gcggatcctg atgatccagg
    13021 atggcctgca gtgtgcggcg cccggtcagc acggtctgtt cggcagcggg gctgaggggt
    13081 acgcgggtgg tgatctcgtc gggcgacggc atccggtcga aggccgcgat gttgaggttg
    13141 tcggtctggg cgctggacat ggcatctcgc gaggggtgga gcgaatccgg cattctaacc
    13201 gcagacgggg acgaaatccc aagggctgat cggttcagcg cccgcgacgc agaggatggc
    13261 gggcgagggc gaggacgctg ggcaggcgca cgtccaccca cgcgggacgg cgcaggctgc
    13321 ggctgatcca gacggtcttc atgcccaggc ggcgggcggt gcgcaggttg tcggcgctgt
    13381 cctcgacgag gatgcagcgc gcgggctgga ggcgctgctg gcgcagcagg tggcggaagc
    13441 cgcggatgtc gggcttgggg tggaagcgca tctgttcgac cgcgcataca tcgctgaaat
    13501 gatgacggac gcccagcacg tcgaggacgg cctcggcata acggcgcggg ccgttggaga
    13561 agacgatctt gcgtccgggc aggcgccgca gcaggtgact gagggctgca tcggccacaa
    13621 ccatgcggtg cagttcgggg aactggtggg tgtcgtggag gaagtggtgg ggattggtgc
    13681 cgtggtggcg catcaggccg gtgagggtgg caccgtagcg ccgccagtag tcgacgcgca
    13741 ggcggttggc ggcagcctcg tccagggcca gatggcgcat gaggtaggcc gtcatgctcc
    13801 ggttgatgtg ggggaagatg tgcggactgg cgttgtgcag cgtgttgtcc aggtcgaaga
    13861 gccagacggg cttcatggcg ctcgggccgg actcagcgcg gcggccgttc gaagcggacg
    13921 atggcgcgtc cgtcttcggc gactttgggg gccaccttcc aggcgtggcc atagaccact
    13981 tcgacggtcg cgggcagacg tccgttgcga cgcagggcct cgtaggcggc acgcaggccg
    14041 gaccagtgtc ccttgccgct gagcccccgg gggcggccgg ccgatgcgca ggcggagccg
    14101 ctcaggcgca ggtcgcgcag caagtcgtcg agggaggcgt aggtgaccgt gagcatctcc
    14161 atgtccatca ccgggtcgga gaagccggcc tgcacgaggg catcgcccag gtcatgcatg
    14221 tcgatgaagc ggtgcaggcg cgcgccgtcc ggtccgggca ggacgctgcg cagttcgcgc
    14281 agggtgtccg gcccgagggt ggagaacatg agcatgccgc ccacctccag gacccggtgc
    14341 atctccttga gggccggcag gggatcgtgc agccactgga gcatcaggtt ggaccacacc
    14401 agcgatacgg cgccacgctt gagcggcagg gcgcgtgcgt cggcgcagag gaagccgggg
    14461 gcgctcgggg tgccgcggga tcccagcagg cgctggaaaa agccctgctg cggaccgtgg
    14521 gcgtcccgcg cgagggcgag catcggccgc gccacgtcca cggcgaggcg cggcacgtcc
    14581 gggtagcggg cggcgaaggc gggcaggtct gcgccagggc cgcagcccag gtcgaggatg
    14641 cggctggggg cgatgcggat gtagtcgagg cgttcgccca tgcgccgggc cacttcccgg
    14701 gcgagggcgt ccacctcggc gtaggtcgcg gcggcccgcc cggcccggcg ccgcacgagg
    14761 cgcaggtcga gctggaaatc cggcgcggtc atcggcggcg gaaactcagg cggacttgag
    14821 gccgaggcgg gcgaagagcc ggtcgtcggc gtcggcgtcc gggttgtccg tggtgagcag
    14881 gcgctcgccg tagaagatgg agttggcgcc ggccaggaag gcgagggcct ggaagccgtc
    14941 gcccagttcc tggcggccgg cggacaggcg cacgtagctt tcgggcatgg tgatgcgtgc
    15001 cgcggcgacg acgcggatga agtcgaaggg gtcgatggcc tcagcgttgc ccaggggcgt
    15061 gccttcgatc tgcaccaggt tgttgatggg cacggaatcc ggcggcggat ccatgttggc
    15121 cagttcggcg atgaaggcgg cgcggctctt gcgggtttcg cccaggccga gaatgccgcc
    15181 gcagcacacg ttgatgccgg ccttgcgcac cttgtcgagg gtgtcgaggc ggtcctgcag
    15241 ggtgtgggag gtgatgatct ggccgtagaa ttcgggcgcg gtgtcgatgt tgtgattgta
    15301 gtagtcgagg ccggcgtcct tgagctgttc ggcctggcca tccttgagca tgcccagggt
    15361 gacgcaggtt tccaggccca tggccttcac ttcgcggacc atgtcgagca ccggggcgag
    15421 gtccttttcc ttcggtccgc gccaggcagc gcccatgcag aagcgggtgg cacccttggc
    15481 cttggcggcg cgggcgttgt cgagcacttc gccgaggggc agcagggtct ccttttcaag
    15541 gccggtgtcg tagcgggcag actgggagca gtagccgcag tcttcggagc agccgccggt
    15601 cttgatggag agcagggtcg agcgctggac cgcgttggcg tcaaaatggg cgcgatggac
    15661 ctgctgggcg cggaaaatca ggtcattgaa gggcagcgcg aagagggcgt ccacctcggc
    15721 cacggaccag cgtttgcgcg gcgtggacgg gggggcggtg gtgaggggct cgtggggggc
    15781 gacggtggcg gtcatgggca tgcaccctta tagtctgggc tgtaattcaa aattacgaag
    15841 ccttttagtt ttactgatgg ggtctcgcct tgtcaaatca aagcgataga tggcgacatt
    15901 ggctggggtg gctgttgccc caggcctgct tcgcgtgcgg tgcgcccagc ggcgacggcg
    15961 tgctgtgcga tgcctgttac ggcgccctgc cggggcggga ctgcccgcgc tgcccgcggt
    16021 gcgccctgcc acagccaggc ggcggcctgt gcggtgcctg tctgcgtgag acgccgccct
    16081 tcgaggccac ccgcgtggcg ctggactacg cctttccggc cgacagcctg gtgctggccc
    16141 tcaagtatgc ccaccggctc gcgacgacgc ggctcttcgt tgatctgctg gccgcagcgc
    16201 cacgtcccga ggcggatctg gtggtgccga tgccgctgca cccggcgcgg ctggcacaac
    16261 gcggcttcaa ccaggctgcc gagctggccc gtccgctggc gcgacactgg cagttgccgg
    16321 tgcgccttga cggggtggtg cgggacgtga atacggtccc ccaggcgggc ctgccatgga
    16381 aggcgcgggc ggccaacgtg cgcggcgcct tccgcgtgac cggacgcctg gacggccgtc
    16441 gcgtggtggt ggtagacgac gtgatgacca ccggtgcaac acttgcggaa ctggcccggg
    16501 ccctcaaggc cgctggtgcg gtgtccgtgg agaaccgggt gctggcgcgc acgccgccgc
    16561 cggcttgatg ggggaggggc gtgcgggcac aatccgcgcc catgctccat gtcgtcctcc
    16621 atcagccgga aatcccgccc aataccggca acatcatgcg catgtgcgcc aactccggcg
    16681 cccgcctgca tctggtgaag cccctcggct tcacgctcga agagaagcag ctcgtccgtg
    16741 ccgggctgga ttaccgcgat ctcgccgtgg tgacggtgca cgaagactgg cctgcgtgcc
    16801 gggcggcact ggcagggcgg cgttgcttcg cgctcacttc ccacggcggc cggcattacg
    16861 gtgatgtggc gtttgctgac gaggatgtat ttctcttcgg cgccgagtcc cgcgggctgc
    16921 ccgccgagct gcacgcggca ttcgacgatg aacaccgcct gcgcattccc atgcggccgg
    16981 gcaatcgcag cctgaacctg tccaacgcgg cggcggtggt ggtctacgag gcgtggcgcc
    17041 agctgggctt tgccggtggg atctgagcgg cgcagggagg gctattcctg cttgggatcg
    17101 cgggcgagga gctgctcaac cgcctcacgg gcacccagac cgccatggag cacgccgtcc
    17161 acggctgcgg tgatgggcat gtccacaccc agccgggtgg ccagggcggc gacttcccgc
    17221 gcggtgctga cgccttcggc cacgtgtccg agggtggcga ggatgtctgg cagagtcttg
    17281 ccttcggcca ggagcagacc gacccgacgg ttgcgggaaa ggtcgccggt gcaggtgagg
    17341 accagatcgc ccagaccggc gagtcccatg aaggtctccc ggcggccgcc gagggcgatg
    17401 cccaggcggg tgatctcggc cagcccgcgg gtgatgaggg cggcccgggc gttgaggccg
    17461 aaacccatgc cgtcggcgac gccggccgcg atggccatga cgttcttgac cgcgccgccc
    17521 acttccgcgc cgaccagatc atcgttggcg tagaggcgca ggcgcgggtc gtggagcgca
    17581 ttcacccaat gacgcgcgaa ggccaggtcg cgggccgcca gggtgacggc ggcgggcagg
    17641 cctttggcca cttcagcggc aaagctggga ccggtcagca cgccgcaggg ggcgtctccg
    17701 cccagttcat cggcgacgat ctggtggggc aactgggcgg tgcccgcctc gagacccttg
    17761 caggcccaca gcagcggcgt acccggggcc agatcgtgca gggcgcgggc cgtggcgcgc
    17821 aggccggcga ggggtgtgac gaccagatga aggtcggcgc gggcggcctc ggcaaggctg
    17881 tcgctggggt gcagcccggc gggcaagggg atgccgggga ggtagcggcg attttcgccg
    17941 tcactgcgca gatcggcgat ctcgcgggct tcgcggctcc acagggtgac gcggtgctgg
    18001 cggctgaagg cgatggcgag ggcggtgccc caggcgccgg cgccgaagac tccgatgtgc
    18061 atcacaggcc ccagacctgg gtcatgcgga tgaagcccgt gctgccatcc cgatggcgga
    18121 ccttgaccca tccgtcggcg ggtgcgtcgg ccagttccag gacaaggtcc ttggccgcct
    18181 cgaaggccag gggggcattc ccatccggct tctggcggat ctcggcgcgg gcggcggtga
    18241 cgatcagggt gcgtttctca gacagctggc ggcgttccag ccagagcagg ctgccggccg
    18301 gttcgcgcac cttgacccag cgatcgacgc tgacgaccat ctccacgggg gtgtagcgct
    18361 ggatcacgaa gagcggtttg ccctgtttgg aaggcgcatc gtataggatg gccggctcgg
    18421 ccagcgaccg gaaatcggag gcgctggccg gggtggccgc cagggcgcag ggcagcgcga
    18481 ggagcgctgc gacagtcagg cggcgcagcg gcattactgg accggaatgt cggtgccggc
    18541 ggaagcctgg cggctctgct ggaacagggc ctcgaagttc accggcgcga ggatcacggg
    18601 ctggaagccg gcacggctga cggcgtcgga gatggcttca cgggcgtagg ggaagaggat
    18661 gttggcgcag ccgatcatca tcagcggctc caggtcttcc tggggcacgt tgcggatctg
    18721 gaagatgccg gcctgggcga cttcgacgag gaacacggtc ttgtcgtcgg gcagcttggc
    18781 ggtgaccgtg acggtcaggt tgacctcgaa cacgccgtcg tccaccgcgg tgctttcggt
    18841 gcgcagctgc acgccgatct gcggggcttc gcgctccagg aagatctgcg gtgcgttggg
    18901 cacctcgacg gagagatcct tgacgtaaag cttttcgatg gagaagacgg gttgttgcgc
    18961 ggtttcggac atggtgggtt ttccgtttgc gtcggtggga tgaatgaaat gatgggggcc
    19021 gctcaggcgc cttcaccgcg cagcagcgga tcgaggcggc cctgatggtc cagttcgaag
    19081 aggtcgtcac agccgcccac gtggaaatcg ccgatgtaga tctgcggcac ggtgcggcgg
    19141 ccggtgcggg acatcatctc gtcacggcgg accggatcca ggtcgatgcg gaccttctcg
    19201 atctcggtga cgcccttgcg ccgcagcagc ccttcggcgc gcacgcaata gggacacacg
    19261 ccggtagcgt acatgaggat gtgtggctgc atgatgactc aggccttctt cttggacttg
    19321 ccgctgcggc ccaggggcat gttggcctgc tcccaggcct ggatgccgcc gtccaggttg
    19381 tgtacccggg tgaaaccggc cttggtgagc acgttgcagg cgttgaccga gcggccaccg
    19441 ctctggcagt tgacgatgac cggcttgtcc ttgaaggatt ccagctcgcc aaggcgcttt
    19501 tcgagatcgg ccagggggat gtggcgggcg ttgggcacgt gaccggcggc aaattcgccc
    19561 gcgtcgcgca catcgatgac gagggcgtcc tcgcggttca tgagctgcgt cgcctgggcc
    19621 ggggagatgc cctggccgcc gcgttgcagg gaggtggcga cgagcaggct gccgctgact
    19681 gcggcgagca gcgcccaata ccagttttgc tggaggaatt ccacgaatcg gactccggaa
    19741 cgggaagggg ttgaacgggg gtggacctca gcctttggcc gagtcgccgc agaagacttc
    19801 gcgcatcatg acgatgagct gcagggtgcg ggtgtcgccg acgcggtaat acacccggtt
    19861 ggcgtccttg cgggtgagca gcacaccctt gtcgcgcagg atggccagat gttgggagat
    19921 gttgctctgg gaggtgccga cggcttcgac gatttcctgc acgcacgctt cctcggcgcc
    19981 gatgacgcag aggatcttga ggcgcaaggg gtgcgagatg gccttcagcg cacgggcggc
    20041 ggtctcgatg tgttcctgct tggcaatcag gtcgacgatg gggctggtca cgaggctaca
    20101 tctgctctgg gtctgcaata ttataaaata ccatctttcc cgctccgggt gcggcgcgat
    20161 ccttttcgac gggccctcgc cgaccgcccg tcgcggccac caggacggcc gcgcgccctg
    20221 caggcgggga acctttcggg tttatactcc tcactcctga cggatccccc ggaatggccg
    20281 gtgtgcggtg ctcgggagtc gccgacgtgg tccttgccgc gtcccccacg gagagtttat
    20341 gcgcagcaag ttgcaacagg ccggtctggt gttcacgggg ctgtgtgccg gcatcctcat
    20401 cagtctcaat ttctccgcca acgcggacaa gaacagcctg ccaccgctgc cggtggagga
    20461 gctgcgcaat ctggccgacg tcttcaacgc catcaagcag ggctacgtcg agccggtgga
    20521 cgacaagaag ctgatcacct atgcgatcag cggcatgctc tccaacctcg acccccattc
    20581 cacctacctg gatccggaag ccttcaagga attgcaggtg ggcacccagg gcgaattcgg
    20641 cggactcggc atcgaggtgg gctccgagga tggctacgtc aaggtgatct caccgatcga
    20701 ggacacgccc gccttccggg ccggcatcaa ggccggcgac ctgatcatca agctcgacga
    20761 caccccggtg aagggcatga ccctcaacga cgccgtcaag cgcatgcgcg gcaaacccaa
    20821 gaccagcatc gtgctgacca tcatccgcaa gggcgaggcc aagcccctga cggtgaccct
    20881 ggtgcgggag atcatcaagg tccagagcgt caagtcgaaa ctcgtcgagc cgggctacgg
    20941 ctatgtgcgc gtcgtgcagt tccaggagca gacggccgcg tccctggtcc agcatctcga
    21001 ccggctggtc aagcagggcg aactcaaagg cctgatcctc gatctgcgta acgatccggg
    21061 cggcttgctc aatggcgcgg tgggggtctc ggccgccttc ctgccgccca aggcgctggt
    21121 ggtgtccacc gacggtcgca ccgatgacgc ccgtcgcaag tatttcgcgg tgcccgacga
    21181 ctacctccgc ggcagccggg atgactacat ccgcaatgtc cctgcgagca tcaagacagt
    21241 gccgatggtg gtgctcgtga atggcgggtc ggcgtccgcg tcggaaatcg tggccggcgc
    21301 cctgcaggat cacaagcggg ccgtggtgat gggcacccag agcttcggca agggctcggt
    21361 gcagaccatt ctgcccctca acagcaacgc cgccatcaag ctcaccacag cccgttactt
    21421 cacgccgagc ggacgttcga tccaggccaa gggcatcgtg ccggacatcg tggtggaaga
    21481 gacgcccaac ggctcccgtg aacgcctgcg cgaggccgat ctcgaccgtc acctggtcaa
    21541 tgccggcgag ccggacgcgc cgaaatcgga gacgcctgcg tcccgcaagg atccggccgg
    21601 caagccccgg gacaaggaca atcgggacaa caaggacaag cccaaggacg aggcggacga
    21661 ggtcaagccg gcctttgaaa tcgccggcaa ggacgactac caactggccc aggcggtgaa
    21721 tttgctcaag ggcctgcaaa tcctccagaa tagcgccaag taaagggctc tttccgggag
    21781 gcgccatgcg tcgcgaaacc gcttacaagc ttgccggccg caagcatgat cacgcctcgc
    21841 atcacgcggg cagcggcctg accaagtcgc gggagatccg cttcaaggat ctccctcctg
    21901 gccaggccgt ccaggcccgg cgttcgctgg cggcgctcaa gggcgtacgg gtgacggacg
    21961 gcaagcatcc cctcgagctc gtcgttcatt acaacgtgct cgattacacc cttgaactgc
    22021 tggaagtggc cctgcagaac gccggcttcc atctggatcg gcccctgctg gtgcgcctgc
    22081 accgggcgct gatctactac gtggaggaga cccaggtgca taacctgcgg tcgcccgagc
    22141 ggctcatcaa gcagtcccac gaagtctacg tcaaggccta cgcggcgcac cctcacggcg
    22201 atcacgacga cacgccgctg gagctgcgcg agtacaagta ggcccctccc gtcccgtcgc
    22261 ccgtgggggc ccacgggtgc ttcacgccgc cccgtgccga tgaacgacga tcagctcctg
    22321 cgctattccc gacatatcct gctgccccag attggtatcg aggggcagtc cgccatcgtg
    22381 tcggcgcgcg tgctggtggt gggcgcgggc ggactgggct cgcctgcggc gatgtacctg
    22441 gcggcggcgg gggttggtac gctggtgctg gctgacggcg ataccgtgga tctgaccaat
    22501 ctgcagcgtc agatcctgca tggcgaggcc agtgtcgggc gtctcaaggt ggattccgga
    22561 caggagacgc tgtcccgact caacccggaa acgcgcgtcg aatccctgcc gcgtcgactc
    22621 gaaggccgcg agctggacga gcaggtggcc ttggccgatg tcgtgctgga ttgctccgac
    22681 aacttcgcca cccgccacgc catcaaccgg gcctgtgtgg cccatcgcac gcccctggtg
    22741 tcgggcgccg cggtgcgttt cgacgggcag ctctcggtgt ttgacctgcg tactgacgcc
    22801 agcccttgct accactgcct gtttcccgag ggcgaggacg tggaggaggt gcgttgcgcg
    22861 gtgatggggg tcttcgcacc cctcaccggc atcgtcggga ccgtgcaggc ggccgaagcg
    22921 ctcaagctgc tggccggctg tggggagacc ctcaccggca ggctgctgtt gctggatgcg
    22981 ctggagatgg actggcgctc ggtgcgcctc acgcgggatc ccacgtgcgc ggtgtgtgcg
    23041 tcgcgctgac cacggccgga acgaccttgc ctgtgttcag gcaggcttcc cgatcagcgc
    23101 cttccagagc agtttgaacc cgagtttctg ctgggcccag aagtggtcgc gggcgttgga
    23161 ttcctccggc gatgcctcgt gcaggccggt ggcgggccag cggcgttcca ggtaaagcgt
    23221 cccgaagagg acatcccaca cgggcagcag gatcgcgaag ttgcagccgt ggcgatagcg
    23281 cggaccggcg ggcaggtcga gggcgtgatg ccagcggtga tacagcggac cgaccaccag
    23341 gtagtcgccg atgggcccga agtcgaagcg cgtgttggcg tgggagaggt tttccaccag
    23401 gcgcagggcg atcagcaggc cgatgaagtg cccaggcggc acgccgatga acagggccag
    23461 gccggcaaac cacagaccgg cgatgaggtc atccgccaga tggttgcggg aatcggtcca
    23521 gaaggacatc tgccgctggg aatggtggat gccgtgcagc gcccaccacc agcggaacac
    23581 atgggacagg cggtggcgcc agtattcggc gaaatcgagg atcaccagat acagcaggaa
    23641 gctgaccaac ggatgatgat ccagggccgg cagcagatct tccagctgac gcgggagcag
    23701 gtcgtggaag cgcagccagc tttccacctc ggcgaacagc ggggcgagca gcagaaacac
    23761 cgccagcggc acgatgccca ggcgattgag gaaggtgtag agcatgtcga cccgcacggc
    23821 gcggcgcgcg gcccagcgct cggccggcca cagggcttcg agagggcgca ggaggaggta
    23881 ggcgacggcc agctccagca ggccgaggag aacgaattcg acaccgtcga agcccaactc
    23941 cacaaacgag cccaatccca gtgcaaacat caccggctgc acgagggcgt cgaagagcca
    24001 ggcctggagg gcgatgaagg cgtcggtgag ggactggatc atggccgttc cggaatcgtg
    24061 gcgaagcaga agcccttgcg ctggagttcg ccgatgagtg ggtcgagcat cggggcgaag
    24121 gggtccttgc gtgaccagat gcccaggtgg gccatcagga tgtcgccgtc cttcagggtg
    24181 tcgagggcgc ggcggaccag cacttcgttg gggaaggcgt cggacggcag ctcgtcgccg
    24241 aggaagccgg cgggcgccca gcccacgtgc cggtaaccgc aggcctcggc ggcctggagg
    24301 gtgaggggtg tggtgcggcc gccgggggcg cgccacaggg ggtcgagggg gcggccggtg
    24361 agctcggcga agcgccggtc gggctggcgg agctcctcgc agacctgcgc cgaatccagc
    24421 tggacggtgc tgccgtccat caggcggtag ctgacgcggc cgtcggcacc gtccttgcgg
    24481 aaggagccgt ggcgccaggt gtggctgccg aaggcgtgcc cctcggcgac gcgggctttc
    24541 cagaagggcg cccagctggc gtcgagggca tggtcaccgc gcagggtctt ttcctgggcg
    24601 acgaagaagg tggcgcggat gtggtgacgg gccaggatgt cggcgatcgc ctcggcgtgg
    24661 cgcatgttgc ccgtgtcgag ggtcaggtac agggtgcccg ccgggcagtc ggccttgccg
    24721 gcgccggcga cggccggcac gagggctgtc agggccaggg cggcgaggtg tttcattgac
    24781 gcggggcgtg gttcaggaag aacacgccgt ggggcgagcg gccgaccggg atggagcggg
    24841 cgagcttctt ctgctccaga tccaccacct gcaccttgcg ggcgaagcgg gcggtggccc
    24901 acagctcctt gccgtcggca cgaatctcca tgtcgtccgg gccgcccggc acgtcgtatt
    24961 tttcgacgac gctgagggtc tgcatgtcca ccagcgagat gctgccgcgc accgtgcggt
    25021 tggacacgaa tacgtggcgg ccgtcgccgc gggcgaggaa gttgtgggcg gccttgtcgg
    25081 tgacgatgcg cttgacggtc ttgcgggcgc gccagtcgat gacctccacg tagtcggcgc
    25141 ccatgatgcc gaccagtagg tatttgtcgt cgggtgtcat gtggatgccg gccggggtcg
    25201 ggccgacggg gagcttccag aggggctgct gggtcttgag gtcgatggcg aggacttcgt
    25261 tggaatcctg cagggtaatg aagacgaact ggctggcctt gtcgaaggcc atgtgggagg
    25321 gtgttttggc ggcgggtacg cgggccacca gcttgaaatc ggcggcggtg tagagatcta
    25381 cccggtcgag gcgcagggag ttggccacga accatttatt gtccggcgag aagccgagct
    25441 ggtacgggtc ggagatgccg tgcaggcgct tctgctcctt gccgttgcga gggtcgaaga
    25501 acaccagttc gttggagatg gcgcaggcaa cgatcactga gctgtcgtcc ggcgtggaca
    25561 tcaggtggtg aggttccttg caggcgggcg cacggccagt ttccttgtag gcgacggtgt
    25621 cgacgaagct gacggtctcg tcggcggagt tgaggaccag ggctgtctcg gcacaggcaa
    25681 gggccggcag gacgctggca gtcagggcaa gcaggaagcg gggaaggcgc atggagcggg
    25741 gacgggtgga tcgaaaaggg ctatgactct aacgcaatgg aatcgacggg cgttgaccca
    25801 ggccaaactc cggcgcggag ggatgtcaaa ttcacggtcg ggacatcccg cgctcaggcg
    25861 gggcgggcga cgatgcgcag atccggcccg atctggcgga cgtcgcggaa gttcagccgg
    25921 cgctgatcgg ccagagcatc gagcgcgggc aggttgaaca gtccgcgggc cgcatcgccg
    25981 accaaggtcg gggcgaggta gagcaccagt tcgtccacgc agccttcccg cagcagggag
    26041 ccgttaagct tgaagccgct ctcggcggtg atctcgttga ggccgcggcg gccgagttcg
    26101 gcgagcaggg ccggcagggc caccttgccg gcgtcattgg ggaggatcaa tacgtccgcg
    26161 ccccgtgcct gcagcgcggc gatgcgggcc gggtcgtcca cggcgccggc gatgagcacc
    26221 gggccgccgt ccaggacgtg ggcgtggagg ggcgtctcga gacgggcgtc cacgacgatg
    26281 cgcaggggct ggcgttcggt ggggatggcg cgcacgttga gctgcggatc gtcgtcacgc
    26341 acggtgccga taccggtgag cactgcgcag gagcgggcgc gccagcggtg ggcgtcgcgg
    26401 cgggcgtcgg ggccggtgat ccactgggag acaccgttgt ggagggccgt cttgccgtcc
    26461 agcgtggcag cggccttgag gcgcagccag ggccgaccgc gggtcatgcg cgagacgaag
    26521 ccgatgttga gttcccgtgc ttcggtttcg aggacgccgc agtgggtggc gatgccggcc
    26581 tcacgcagac gggcgaggcc gcggccggcg acgagggggt tgggatcctc catggcagcc
    26641 acgacgcggg cgactccggc ccgcaccagg gcgtcggcgc aggggggcgt gcggccgtgg
    26701 tgggcgcagg gttcgagggt gacgtaggcc gtggcaccgc gggcccgatc accggcctgg
    26761 gcgagggcgt ggacttcggc gtggggctcg ccggcgcggc ggtgccagcc ttcgccgacc
    26821 acctgcccgt cgcggacgag tacgcagccg acccgtggat tgggggtggt ggtaaacagg
    26881 ccgagttctg ccagctggag ggcgcgggcc atccaccggg tgtcatcgac gctggcagac
    26941 atgatcagga cgtggagcgg gggcgtccgc ggggacgggg cgagacttcg cggatgactt
    27001 cggagaactc gtccacgtcc tcgaaggcgc gatagaccga ggcaaagcgg atgtaggcga
    27061 ccttgtcgag cttcttgagc tcgcgcatga ccagttcgcc aatgagctct gaggcgactt
    27121 cccgttcgcc cagggacagc agcttggctt cgatgcggtc gacggcggct tcgacgcttt
    27181 cggccatgac cggccgcttg cgcagggcca gggagaggct gccgaggagc ttgtcgcggc
    27241 tgaagtcggc gcgactgccg ttgcgcttga ccacctgggg catcttcagg tcgacgcgtt
    27301 cgtaggtggt gaagcgcttg tcgcaggtca ggcagcgacg gcggcgccgc acgatgtcac
    27361 cgtcctcgtt ctcacgggtg tcggtgacct gcgtgttctc ggcgctgcag aaggggcatc
    27421 gcatggaggc tgctgcctgt cagaggctca tgcgccgtag accgggaact tgcggcacag
    27481 ttcggcgacc ttgctgcgga cggcggcgag ggtggcttcg tcgttgggat tgtccagcac
    27541 gtcggcgatc aggtgcgcga cgcgttccgc ttcgatctcg gtgaagccac gggtggtcat
    27601 ggccggcgag ccgatacgga tgccggaggt gacgaagggc ttctggggat ccttggggat
    27661 cgcgttcttg ttcacggtga tgtgggcgcg gccgagggcg gcttcggctt ccttgccggt
    27721 gatgttcttg gcgcgcagat ccaccaggaa cacgtggctt tcggtgcggc cggagacgat
    27781 gcgcaggccg cgctcctcgc cgaggacgcg ggccatcacg cgggcgttgg cgatgacctg
    27841 ttcctggtag ttcttgaact cgggcgtggc ggcttccttg aaggccacgg ccttggcggc
    27901 gatcacgtgc atcagcggac cgccctgcag acctgggaag atggcggagt tgatggcctt
    27961 ctcgtgctcg gccttcatca ggatcacgcc gccgcggggg ccgcgcaggg tcttgtgggt
    28021 ggtggaggtg accacgtcgg cgtggggcac cgggttgggg tagaagccgg cggcgatgag
    28081 gccggcgtag tgggccatgt ccacccagaa gatggcgccg acttccttgg cgatctttgc
    28141 gaagcgttcc cagtcgatgc gcagggcgta ggcggaggcg ccggcgacga tgatgcgcgg
    28201 cttggcttcg cgggccagtt tttccatcgc ctcgtagtcg atctcttcct tgtcgtcgag
    28261 gccgtaggcg accacgttaa accacttgcc ggacaggttg aggggcatgc catgggtgag
    28321 gtggccgcct tcggcgaggc tcatgcccat gatggtgtcg ccgggcttgg cgaaggccat
    28381 cagcacggcc tggttggcct gggaaccgga gttgggctgc acattggcgg cgtcggcgcc
    28441 gaacagggcc ttgaggcggt cgatggcgag ctgctcggcc acgtccacgt gttcgcaacc
    28501 accgtagtat cgcttgccgg gatagccttc cgcgtacttg ttggtgagct gcgagccctg
    28561 ggcttccatg acggcgcagc tgacgtagtt ttcggaggcg atcagttcga tgtgatcttc
    28621 ctgacggcgg ttttcggcct caacggtctg ccacagttcg gggtcgacgc gagcaagggt
    28681 gttttgcttg gaaaacatgg caatccaggg ggttagtgca aaaatgaagt gtaacacgcc
    28741 cccccggcgt tgccgaaccc ggggtttccc ggatcacagg cggaagtggc tgacgcgggc
    28801 ttccagttcg tcggcgagtt gttccagctg ggtggtctgg gcgtggctgt cgcggatgat
    28861 ctggttgttc tgttcggcca tgcgggcgat ctgctctacg ttgcgggcca cctcctggct
    28921 cgcggaactc tgctcgaggg tggcgtcggc aatgtcgttg acggcgccgg tcgtcctcgc
    28981 gctctcgctg cggatggtct cgagcatggc gcccgaagca gtggcgacag ccaccccgtt
    29041 ctgaacgccg gttgtgatgc cctggatggt gtcgaccaca cgctgggctt cgtcctgcac
    29101 cagggtgacc gtctcggaga tctctcgcgt ggaacttgct gtgcgttcgg ccagcttgcg
    29161 cacttcgtcg gccacgacgg cgaagccgcg gccgctctca ccggcccgtg cggcctcgat
    29221 ggcggcgttg agggcgagga ggttggtctg gtcggcgatg tcgttgatga tccggccaat
    29281 gttggaaatg cgggcggagc gctgggacag gctgttgacg gcggcgctgg cggtgtccat
    29341 gtcggcggca atgcggccca tgccatcgat catctcgcgc atgcggtgct ggccgtcgtc
    29401 ggcgtgggcg ctggcgtcct gggtcatctc gcgggcgagg gcggcgctgt cggaaacgtg
    29461 ggtgatgctc acggacagtt cttccacggc ggcggcaaga cccgaggccg actcgctctg
    29521 gcgggcggtg ctggtgacga tgctttccga gtcggcacgc agggaatgag cggccccctt
    29581 gatggcattg gcggtgtggg tgacgccgcc gatcagaccg gccatctggc gggtcgtgct
    29641 gtccagtgcc cgggtgatga gttcgacttc gttgcgagtt gggtccggga cggcggggaa
    29701 ggcgacgttg agttcgccgc gaccgatgcg ttcggtgacg gcgaccagtt cgcgtatcgg
    29761 cgcaatgcgt gaacgggcga tgagatagag cacggccacc agcagggctg cgccggcggt
    29821 gcagagcagg atcagcaccc ggagcagttc gcgggcatgg acggtgtact cgtccacaaa
    29881 gctgccggtg gcaacgatcc agccccacga cggggccgtg tcgaaggcga ccatcttggt
    29941 cttttcgccc ccgccgttgg ctggccagtc gtaggtcagg gtgcccttgc ccaggcggat
    30001 ctgctcgccg accacgcggc tcagaaccgg gttgttgagt tcgttgagct tcttgccgct
    30061 cagggtcggg tgaaagacga cctcgctggt ggcggcgtct gcgcccgggg cgatgacata
    30121 ggtgtagccg gtggcgccgg agcgggtttc gcccagaacc gcaaagagcc gtgccatgtc
    30181 gctctgaatg tccacacgtg cggcgagggc gccgacgacc ttgccgtcgg cgtcgcgcat
    30241 gggatcgatg gcgctgatga agaaggtgcc attgcgtcgt accacgcccc ggtaggattt
    30301 gccctccagg agggctcgcg tttccttgcc gtcacgcggc agcggtgtgc cttcggtgct
    30361 cttgccgtcc ttgtcgcgga gcagggtggc ggcgcgcacg aattcgtcgc ccacccgcag
    30421 cagcaccgcg ggatctgcgt cgatctggtc gcgcagggtt tccaggaggg ccttgttgcc
    30481 attgaggata cgttcgccgc tgcggaccac ggggacctgg tagctgcccg atgccatcgt
    30541 gtcggggccg atgcgcaggg gcccgaaggc ccgctggacc cgcccgaggc tactgccggc
    30601 gcgggccacg gcgccggcgt gattgagctc gagcatgcgc acgacgccct ggatctgatg
    30661 gccgagctcg tcgcccgtct tggccagatc ggctttctcg gcgaagtagg tggtgaaggt
    30721 gaccagggtg ccgaagacga tcaggctgat gaggcccgcg gcgagggcga tctgatggac
    30781 gagggacagg cggttcagtc tggacatggc gattctcccg cgcccgtcgc gcaagctggg
    30841 cgatgtggcc gggaaaatcg gcggtccatc agaaaacttt aatttgcgct gcggtcctca
    30901 gctgttgggc aattcatcga gggcgccgtg caggtggctc tggcgggccc attcgatgag
    30961 gctccagcgg ccctcgtccc accccagcca gttgagggcc gcattgggaa tggtgaagtc
    31021 gcgcggcgtg tcgaggggct ttccggtggc caggcggtgc gccacgtcga gtacgccacc
    31081 gtgggtgacg accagcacct tgcgccccgg gtgggtggcg gcgatgcgtt ccagggccgc
    31141 acggatacgc gcggcaaatc cattgaggct ctcgccgccg ccgggaaaga cgaagtccgg
    31201 atgacgccct tcgaagcggt ggtaatcgtc ggggaagcgg gcgcgggcct cgtcgtaggt
    31261 cagggcctgg aagtgaccgt agtggcgctc ccgcagggat gcgtcgtggg tgatcgcgag
    31321 ggcgcagcgc tgggcgatgg cctcggcggt ctgacgggca cgcagcaggt cggagctgta
    31381 ggcggcgtcg aagccctcgg ccgcgaggct ggcggcggtg gcgcgggcct gggcgaggcc
    31441 ggtgtcgttg agggggatgt cggtgtggcc ctggaggcga cgctcggcgt tccaggcggt
    31501 ctcgccatgg cggaccaggc agatgcgggt gatcatgagg ctgacgcggg aatgcgggag
    31561 cgcaattatc gcactgccgc cagcgtcggg gccgatccgg cgccggtcag tgaaatccct
    31621 gattgcgccg gggccggact gaccgataat ccgccagcgc gtttccgccc ggaaatggca
    31681 ttcataccca aaaacacaag gagacgggaa ggtgcaagcc ttgctcaagc ttagccgggg
    31741 gatcgacgcc ctgacggatc gggtcggcag gaccacgacc tggctggtgc tggtcgtgac
    31801 cctgatcagc gcgatcaacg cctttgtgcg ttacgcggtc aattacagtt ccaacggtct
    31861 gctggagatc cagtggtacc tcttttccgc gatcttcctg ttgtgcgcgg gttatacgct
    31921 gatgcgcaac gagcacgtgc gcatcgacgt ggtgaccggt cacctgtcgc cccgcgctca
    31981 tgcgtggatc gatatcgtcg ggacgctgtt cttcctggcg cccatggccc tgctggtgct
    32041 gtatctgtcg tggccgatct tcgtcaattc cttcctcacc catgagcagt ccagcaacgc
    32101 cggcggcctg acggtgtggc cggtgcggct gctggtgccg gtgggctttg cgctgctgat
    32161 cctccaggcg gcgtcggaat tcatcaagcg ggtggctttt ctgaagggcc tcatcgacga
    32221 tcccagcgcg aagcacaagg gcccatccgc cgaggaaggg ctggccgagg cgatccgccg
    32281 gcaacgcgga gaggaggcat aaatggaatt cctcgccgcc aacatggcgc ccctgatgtt
    32341 cgggtcgctg gtgatcttcc tgctgttcgg ctatccggtg gcgtttgccc tggctgccaa
    32401 cggcatcgtc ttcgggctca tcggtattga actggggctg ctctcgccgg ccctgtttca
    32461 ggccctgccg gaccggatct tcggggtgat gtccaatgac accctgctgg ccattccctt
    32521 cttcaccttc atgggcctga tcctcgaacg ctccggcatg gccgaggacc tgctcgacac
    32581 catcgggcag ctcttcgggc cggtgcgcgg cgggctggct tttgcggtga tcttcgtggg
    32641 cgccatcctc gcggcgacca ccggcgtggt ggccgcatcg gtcatctcga tgggcctgat
    32701 ctccctgccc atcatgatgc gttacggcta cgaccagcgt ctggcgaccg gcgtgatcgc
    32761 ggcgtccggc acgctggcac agatcatccc gccctcgctg gtgctcatca tcatggccga
    32821 ccagctcggc cgctccgtgg gcgacatgta ccagggggcg atgatccccg ggctggtgct
    32881 gaccggcttc tatgtggcct acgtggcgct ggtggcggtg ttccgcccgg ccgcggcgcc
    32941 cgcattgccg cccgaggcgc gctcgctgcg gggcatgacc ctggcgatcc gcgtgctcac
    33001 gaccctggtg ccgcccctga tgctgatctt cctggtcctc ggcacgatct tcctgggggt
    33061 cgccacgccc accgaagggg gcgcgatggg ggcctttggc gccctggtga tggccgtcct
    33121 gcggcggcgc ctcaacctga gccttctcag gcaggccatg gagaccaccg ccaagctcac
    33181 taccttcgtg gtgttcatcc tggtgggcgc gcgggtcttc tccctgacct tctacggcgt
    33241 ggaaggccac ctgtgggtcg agcatctcct gtccggtctg ccgggcgggc ggctgggctt
    33301 cctgatcgtg gtgaacatcc tggtctttct gctggccttc tttctggatt tcttcgagct
    33361 gtccttcatc gtcgtgccgc tgctcgcgcc ggtggccgag aaactgggca tcgacctgat
    33421 ctggttcggc gtgctgttgg gggtcaacat gcagacttcc ttcatgcacc cgcccttcgg
    33481 ctttgccctg ttctacctgc gttcggtggc gccgcccagc atcaagacca cccagatcta
    33541 ctggggcgcc gtgcccttcg tgctgatcca gatcattatg gtgggtgtca tcatcacatt
    33601 cccggatctg gtgaccatgg gcctcgacag ccaggaaaag gtggacgcgg acgccatcga
    33661 gatcgtcgtg ccgcaggggg atgctccgga cgatgcgccg gtgcagtttg acgcgggcaa
    33721 ggacggggac gcgccccatg caggtgacgg gcaggctggc gccggcgagc agaaggatgg
    33781 catcccggtt ccgccggccg atgatgacgc gaccagggct ctggagaaag ccctgcggga
    33841 ggaccgcagg taaggggcgt gcccgccatg gcccgcaaaa cggaagcccg acctgcgagt
    33901 cgggcttctg ctttcgggaa cgggatgcgt ccgggcgggt cggaagggtg gcaccgggat
    33961 gtcctccggt cggtccctgc gccgcgtgac cgggcaggga ccggtcctcg gggctacccg
    34021 gtgccgggtg cagcagatgt ctgcaaccgt ccggctttaa cgggagtttc catgaccgcg
    34081 gcagaccctg tcagcgtaat gtcgatgcaa atgtaattca cctggttttt ccttttgggt
    34141 gtgacaaaag tcgtaggtgc ccggtccagg cggtcactgc cgacgtcgca cgggtggatg
    34201 tgcaggggct ggcgagcggg tatattccag cgtcgtcgcc cggccgccgt gttattcccg
    34261 atgcctgccg cctcgtcggc ggctgactgt gcccgtccgt tcggatcagg caactgacgc
    34321 gatctcccca acatttcccg caggattctt gccatggcct tgctgacttc cctgtcaccc
    34381 gatgtgtttc ttgaacggct ccgtgctgcc cttgccgagc cgcgaaggga tcatccggag
    34441 ctggcgcggc tggcgcggcg gctggcggcc ttcattgccg cagaggatct gccggaccgc
    34501 ctcgatgcct ggcatgccct gatgacctgg ttggctggcg ggcgactgtg gcaggacgtc
    34561 tatatcgatg tggtggatgt gcacggtccc gccacccagc gtctggcggt gctgatggac
    34621 gtactggccg gtgacgctga gctggccggc gcactccggg cggccctgcg cggattgctc
    34681 gacgagaccg acggcgccaa ccttttcggg gaggtgggga ttcccagtga ccggggcttc
    34741 ctgagtgagt tcggcgatcg cctgactgcc aagctgttgc cacagccgcg cgtcgatcat
    34801 gatctcgcgc atcttgtggg ccgcagctac cggcgccggc gggatctgga tctcgtccgc
    34861 gccctgcatc cgtccaccct gcagcgtctg atggagctcc tttttccggc cgacggtccg
    34921 gagatgaccc gtgccctgcg acgcgccatg gcggacggtt tccgcctgct gctggcgcgt
    34981 gtggcgggcc agggattgtc ggagaagctg cgggcccggg gcagcgccca ggcggtggcc
    35041 gacgtgccct ttgcgcggct cgcccgcttt ggcgaagcgc tggtggccga ctgggagggg
    35101 ggcgccctgc ccgatgcggc catggacgcc tggcaggcgc tgtcgcatgc ctgccgtgcc
    35161 gaaatgcagg tcgtcagccg gcgcctggag tctgaaggca tcagcgtgga catcgtgtac
    35221 gggcttgatg tgctcgaacg ctgcctgaat cgtctggatg ccctggtcgc ggtgatggtg
    35281 gaaggcggtg ccgcgccggt gctggctctg gtccagcaac tggtgctggc ggcccaccag
    35341 agcagcagcc tgcgccatct ggtgcgcatc aacctgcagc ggctgcaacg gcgcatcgtg
    35401 gatcgggcgg ggcagacggg ggagcactac atcgccgata cgcccggtga gtaccgccac
    35461 atctggacag ccgcggccgg gggcggtctg ctcaccgtcg gcacggcagc gatgaagatg
    35521 accattgccg gtgccgggct ggctctgttc ctggaagggc tgggctccgg gctcaactac
    35581 gcggtgagtt tcctgctcct gcaggccttc gggctcattc tggccaccaa gcagccggcc
    35641 atgaccgcag cggcctttgc ggccatcatc cgcgaccgcc agggggccga acggctcgac
    35701 agcatcgtcg acgcggccgc gcgtatctgc cattcccagc tggcagcgac aatcgcgaat
    35761 gtcgtggtgg ttgccttcgg ggcctatgcc ttcaacggcc tgtgggcctg gatcaccggt
    35821 catgccttcc tcacggccag cgaggcacgc tatgtgttca aggccctcag tccggtggat
    35881 agcggcaccg tgttctacgc ggccctcacc ggggtgatcc tgtaccttgc cgcgctggtg
    35941 ggcgggtggg tggacaactg ggcggtctat caccggctgc ctgaggccat tgccgagcat
    36001 cgcctggggc agcgggtcgg acggctgcgc atgctgcgtc tggcgggcat cgtgtcgcgc
    36061 aacatggcgg gatgggcgac caatgtttct ctgggcctga tgctcggcat gacaccggtg
    36121 ctcggccggt tcttcggact gcccctggat gtgcgtcacg tgaccctcaa ctcgggcatt
    36181 ctcagtctcg ccgcggccgg catggacctg caatggatcg gcaccggtca cctctcgctg
    36241 gcgatcgccg gggtggcggt gatgttcgtc ctgaatctgg gggtgagctt cgccctgtcg
    36301 ctgctcacgg cgatccgcgc ctacgaactg cccgccgaag acctgcggga gctgcgccgc
    36361 cgcctgtggc agcgtctgcg cacccggccg caggatttcc tgatgccgcg gcggttgccg
    36421 ccagcgccct gagcgtcccg cctcaagctc gctgccgccg ggccgataag cacccagcgg
    36481 cggggcaggc cggcacccag ggggtgcacc ggctccgcct ccggagacca ccctggcaac
    36541 cgcgggttgc caagcgagcg aagcatgaat gcgccagaac gagttccctt tgccggttcc
    36601 cgcgccgtgg cgggcgagct ggcctgcatc gtccgggtca atgaagagat caaggcggtg
    36661 gtcgccacgg ccacgcggat caacctgatg gccctcaacg caatcttcct ggccaagcgg
    36721 gcgggtaatg cggctctggg atttggcgtc ctgtccaatg agctgcgccg gttcgcccag
    36781 gatctgaccc gccacatggc ggtgctgaga gacatgacgt ccggctcggt cggcagcgtg
    36841 acgggcgtcg tccagcacca gcgcaactcg cgcatcctgt ccgaggcgat ccgcctgagc
    36901 ggtgagggtg tgccgggtgt ggacatggcc cggcggcgtt gcaatcagcg cctggcgcgc
    36961 gaactcgcca gcttgcgtgg actcgaccag agactgcgcg cggcagtgga ggaaacccag
    37021 cagctcgtcg aacttggcgg cgtgctggcc cgctcggcca agatcgaggc ggcctacggt
    37081 ggtggcttca gcccggcgct gatgcaggtg tcgacggatt ttgcggacgt gatcgcccag
    37141 atcaagcgct ccctggaaaa cctcagcaag gaacggctcg tcaaggactg aaccctatga
    37201 aacaggccca ctacatctat caggacggcg atcatcgctg gctggccatt gcccgtgacc
    37261 cgaacaagcc gggctacgtc atcgatacca acgaatacgt ggtggccgac gaggaggctg
    37321 ccctgctgct cgacccgggt ggcatcgaaa tcttccctgc ggtgttctcg gccctgtcgg
    37381 gcgaatgtga tccgacgcgc ctgatgggca tcttcgcgtc ccaccaggac ccggacatca
    37441 tctcgtccct ggccctgtgg ctggagttca agccggacct gaagtgctac acgagctggc
    37501 tgtggtcgtc cttcctgccc cacttcggcg gagcgtcgga aaccttcgtg tccattccgg
    37561 acggcggtat gaccatcaag gtgggcagcc tggatctgca ggcggttccc gcacactacc
    37621 tgcattcctc gggcaacttc catctctacg actccaaggc caagatcctc ttttcggggg
    37681 atgtgggcgc ggccctgctg ccgcccgaca acaacggcct gtacgtggag gacttttcgc
    37741 gtcacatccg tcatgccgaa gccttccaca agcgctggat gggctccgac cgggccaagc
    37801 gggattggtg cgagcgggtt tcgcggctgg agatcgacat gctcgtgccc cagcacggag
    37861 ccatttacca gggcccggat gtgcagcgct tcatcaactg gtttgccgcg ctggaggtgg
    37921 gtgtgctgcg gccctgatgc gggcaggccg cgcgccggtc tcaacgcccc gtgcgccgga
    37981 aggtccggtg gcggggcgta ttgttcttgt cggacggcaa ggtgtgcggg tggtgacttt
    38041 cgtagaatga tccccgtcct agcttccgtc acgccgttac ccgccggcgg tgtgtgccat
    38101 gtacctcgcc atcaagcacc tccatatcac ctgcgtcgcg ctcagcctgg cgggcttcct
    38161 gttgcgtgga ctatggatgt gcgccgactc acccctgctg tacgcgtcgc tgacgcggcg
    38221 cctcccccat atcgtcgatg cgctgttgct gggcagcgcc atcgcgctgg ccgtgatgag
    38281 cggtcaggcg ccattcgtgc aggcgtggct gacggccaag gtcgcagggc tgctggtgta
    38341 catcgtgctg ggcgcgcttg ccctgcgtcc gggccgcccg aagcccgtac ggctgggggc
    38401 attcggcgcg gcgctggtga gcttctcgta tatcgtcagc gtcgcgctga ccaagaatcc
    38461 ggcagggtgg ctggcgatgt ggggttgagc ctcaggtcgg cccgtggttg agcagcgcct
    38521 tgagtccggc gatgtcgagg atgcggatgt gcttctgctg gaccgacacg aatccttcgt
    38581 cctgaaagcg cgagaaggcc cgcgagacgg tctcgagctt gagcccgagg taggagccga
    38641 tctcgtcgcg ggtcatgcgc agatggaact cggatggcga gaagccccgt gccgtgaagc
    38701 gctgggacat gttgaggaga aaggcggcaa ggcgttcctc ggcgcgcatg gagccgagca
    38761 gcatcatgac gccgtggtcg cgcacgatct cgcggctcat gatcttgtga aactggtgct
    38821 gcaggctttc cacttctcgg gagagctctt cgaggcgccc gaaggggatg acgcagacct
    38881 cgctgtcttc gagcgcgatg gcgttgcacg tgtggtgttc gatgctgata ccgtcaagcc
    38941 ccatgacctc gccggtcatc tggaagccgg tgacctggtc gcgaccgtct tcgaggatca
    39001 cgtcggtctt gaaggatccg gtccggatgg cgtagatcgc ctcgaacggg tcacccgagc
    39061 ggtagaggtg ctgatgacgc ttgattttcc gacggctccc gacgagctcg tcgaggcggt
    39121 cgatctcgtt cgggtcgagc ccgaagggca ggcacagttc ccgcagattg cattgggaac
    39181 acgcgatctt gacttccgtg acggacagcg ggataaccgg tttcgtgtgc actcagattc
    39241 acctgaactt tggggccttg cggttggcga gcaacctttg aagccctttg atccacatca
    39301 atacgatttc cgggggattc tgggatcgtt ccacgcaccc tacaatgcgc tcccatgaac
    39361 acgatccaca aagacctgat cttcgatccg cagctcattc gcaagtttga cgtgaatggg
    39421 ccgcggtata cgtcctatcc caccgctgac cgcttcgttg aggccttcaa ctccgaagcc
    39481 ctgtgcgact ggttgggcaa gcggacgatc gggggggtgg gaaagccgct ctcattatac
    39541 ttccacatcc ccttctgcaa tacgatctgc tactactgcg cctgcaacaa aatcatcacc
    39601 aaggatcacg ggcgttccgc caagtacctg cgctatgtgg cccgcgagat ggagatgcag
    39661 gttgcccatc tcaccggacc gcgcgaagtc ctgcagcttc acctgggcgg cggtacgccc
    39721 accttcctct cccacgacga gatgcgggag ctgatggcca acgtccgcaa gcacttcacg
    39781 ctggtgccgg acggggagtt ctccatcgag gtcgatccgc gcaaggtgga cgaagccacg
    39841 gtggcattgc tggccgagct cggtttcaat cgcatgagcg tcggcgtgca ggatttcaat
    39901 cccgacgtcc agcgcgcggt gaaccgggtg cagagcgagg aggagacccg tctggtcatt
    39961 gaatccgccc gcgcccacgg gttcaagtcg gtcagcgtcg acctgatcta cggactgccc
    40021 aagcagaacg tgatcagctt caaccgcacg ctggagcaga tcctggcgct ctcgccggac
    40081 cgcctttcga tctacaacta tgcccatctg cccgggctgt tcaaacccca gcggcgcatc
    40141 cacgacgccg aactgcccaa tgcggaggcc aagctgcaga ttctgcaact ggcgatccgc
    40201 cgcctgaccg aggccggcta cgtgtatatc ggcatggatc acttcgccaa gccggacgat
    40261 gaactggccg tggcccagcg tcaggggcgt ctgcatcgca acttccaggg ctattccacc
    40321 tacgcggagt gcgatctgct ggccttcggc gtctccgcca tcggcaaggt cggaccgacc
    40381 tacgcgcaga acgtcaagac tctggacgag tactacgaca tcatcgacgc agggcgcctg
    40441 ccggtcctgc gcggcatcga gctgacgccc gacgacctgc tgcggcgctc catcatccag
    40501 gcgctgatgt gccacttcga gctgtccatc gagtccatcg aaattgccca tctgatcgac
    40561 ttcccgcgct attttgcggc cgagctggcg gatctgcgcg agatggagaa gaacggactg
    40621 gtgcagatcg acgataaatg gatcagcgtg cagcccgccg ggcgtatgct ggtgcgggca
    40681 atcgccatgg tgttcgaccg ctatctgcgg gccgaccggg agcgcacccg ttactccaag
    40741 gtgatctgag cgtcgatggg cgtctgcccg atgcgggctg atcgcgcgct cccgcgtggt
    40801 gtagcctgtt ccgcggcgca cgctggaatg tcccgacctt tcatgatgga atgcgccccg
    40861 tgtctgcggg gcgtttttca tgcgcacttg agaatccatg cctgaaaccg gttatctcgc
    40921 cgtcttcctc atcggtctgc tgggaggcac ccactgcgtg ggcatgtgtg gtggcatcgt
    40981 cagtgccctc accgtgcgca tgcccgggga gcagcggcgt gactggcctc tccacgtggc
    41041 ttacaacttc gggcgcatcg ccagctacac cgcggccggt gcggcgctgg gcgccctggg
    41101 cagcgcgggc atgctcctcg acggcatgct gccggtgcag ctgggcctct atgtggctgc
    41161 aaatctgatg cttgtcgccc tggggctcta cctgacgggc ttcacccgcg cactggccgg
    41221 cgtcgaacgg atcgggcaac gcctatggac gcgcatccag cccctgacgc ggcgcttcct
    41281 gccggcgcgc tcggttgcgc aggccctgcc cctggggatg ctgtggggtt tcctgccctg
    41341 tggcatggtc tatagcgtgc tggccacggc gctggtcacc ggatcagcgg ggcggggggc
    41401 gctgctgatg ctgtcctttg ggcttggaac gctgcccaac ctgctcctcg ccgggctgct
    41461 cctgcgccat ctgcgcgagg tggtgcggaa ccggtcggtc cgcctggtgt ccggactggt
    41521 ggtgctgggc ttcggtgtgt atggcctgct caatacgtcg agcctgggca acaagctgtg
    41581 gaatggcgtg gtgtgccatg tgtgagggct gcggccccga gacaaggtga aggcaatgaa
    41641 gatcctgttg gcggtggatg gatcgccggt gtccctgcgg gcggcggagg aagtggtgcg
    41701 cctggcgcgg gcactgaaat ccgagccgga cgtccacgtg gtgtatgttc atctgcccat
    41761 cccgctggac tttgcaaccc gtcatgtggg ccgtgaggcc gttgaggcct actaccggga
    41821 cgagggtgag gcggtattgc aggcgcccct ggctgcattg acggcctccg acctcatgcc
    41881 gcaacgtcac ctgcacgtgg ggcagccggc cgatgtgatt ctgcaactgg cgggcgaact
    41941 gggctgcgat ctgatctgcc tcgggcgtca cgggcgcggc gccatggccg accttctgct
    42001 gggctccgtg gccaaccggg ttgttcagcg cgcaccgatg ccggtactcc tggtgggctg
    42061 agagcagatc atgtcggacg gcccgttgcc cgaatccatg gatgagctgt ccatcacggg
    42121 catgagttgc gccgcctgcg cggtgcggct cgagaaggtg ctcaaccgcc tgcccggcgt
    42181 gtcggcgcac gtgaattttg ccaccggccg ggcgagcgtc cggcgtgcga ccggcgcggc
    42241 ggacggcgaa gcccttgtcg ctgcggtacg caaggcgggt ttcgacgtgg cgcccgagac
    42301 cctcgagctg gcactctccg gcatgagctg cgcggcctgt gcggcccgga tcgagaaggt
    42361 gctcaaccgc ctgccgggtg tggaggccac ggtcaatttc aatgcagaga cggcgcgcgt
    42421 ccgctatgtg cccggtctgg cagggcccga cgccatgcgg gccgccgtcg ccaaggccgg
    42481 attctccgcc gagcccgttc ttgcccgcga ccgggaggcg gagcgcgccc gcaaggccga
    42541 ggaggcgcga agcgaatggc gtcagtttct gctggcactg gtcctcagcg cgcccttcct
    42601 gctccagatg cccttcatgc tggcgggtgg cctggacggc catcacgaat ggctgccccg
    42661 ctggctgcaa ggtctgctgg cggcgccggt gcagttctgg gtcgggcggc gcttctaccg
    42721 gggggcgtgg tcgtccctgc ggggtggcgg cgccaacatg gatgtgctgg tggcgctggg
    42781 gaccagcgtc gcgttcttct tcagtctggc cgtggtgctc cttgggcggc acgatcagca
    42841 tgtgtatttc gaagcctcgg ccagcatcat caccctggtg ctgctcggca aactgctgga
    42901 ggctcgggcg cgggcgcgga cctcggcggc catcgaggcc ttgctgcgtc tgcagccgcc
    42961 caccgcgtgg ctggagcggg acggcgcgct ggtggaagtg cctgtcgccc aggtggcgcg
    43021 gggcaccgtg ttcgtgctgc gggcgggcga cgcggtgccg gtggacgggg aggtgctggc
    43081 gggcgagtca tccatcgacg aggcgatgct gaccggcgag agcgtgccgg tgcccaaggc
    43141 ggcgggggac acggtcttcg cggccacact caacggcgcc ggcctgttgc gctgccgggc
    43201 taccggcgtg ggtgcggaga cgctgctggc ggggatcgtg cggctggtgg agcaggccca
    43261 gggcggcaag ccggccgtgc agcggctggc tgaccggatc gcggcggtct ttgtgccgac
    43321 ggttgtcggc ctggcgctgc tcacatttgc cgcctggtgg tgggcggccg gggatcccgt
    43381 gcaggcgttg gtgaatggtg tggcggtgct ggtcatcgcc tgcccctgcg cgttggggct
    43441 ggcgacaccg acggccatca tggtgggtac ggggcagggc gcccgcgccg gcatcctcgt
    43501 gcgcaaggtg gaggcgctgg aactggccga atccctgcgc atcgtggcgg tggacaagac
    43561 cggcacgctc accgaggggc gtccggccct tgtggagacc cgcctcgcca agggcatcgc
    43621 ctacaacgag gccctgcacc tggccgccag cctggagcaa ggcagccctc acccgctggc
    43681 ggtggcactg gtggcggccg cgcgggaggc gggcatcggg ctcgccctgc cggttgcggt
    43741 gcagtcggcg ggtggcaagg gtgtgtcggg cgaggtggcg gggcggcagg tggcggtcgg
    43801 ctcgcctgcc tacctgacgg aggcgggcgt ggcgattccg gcggatcagc tcgatccgct
    43861 gactacgggg ggattgacgc ccgtctgcgt ggcggctgac ggccgcctcc tggcgctttt
    43921 cggggtggct gaccggctgc ggccgggcgc ggcagaggct gtggcgcgtc tgaggcgaca
    43981 ggggctgcgg gtcgtgatgc tgaccggcga tcatcccgcg gtagcccaag caatcgcccg
    44041 gcaggcgggc atcgacgaat ttcgcgccca ggtgctgccc gctgacaagg cggcggcggt
    44101 gcaggccctg cgcaacgaag gacgggtcgg catggtgggg gacggcatca acgacgcgcc
    44161 ggccctggcg gcggcggacg tgtccttcgc catcggcgcc ggatcggacg tggcggtgga
    44221 ggcggctgac gtgaccttgg tgcgcagcga cccggccggc gtggcagacg ccatcgcctt
    44281 gtctcgcgca acgctgggca agatccgcca gaatctcttc ttcgccttca tctacaacat
    44341 cctgggcatc ccggcggcgg cgctggggtt gctcaatccg gtcatcgccg cggcggcgat
    44401 ggccatgagt tcggtgtcgg tggtgagcaa ttccctgctg ctgcggcgct ggcggccggg
    44461 gcaggactga tttcaggagg acaggaccat gcaggaagcg atcattcagg tgggcggcat
    44521 gagctgccag ggctgcgtca ggaacgtgac cggcgcactg tcggcggtgg ctggcgtgac
    44581 ggccgtcgag gtgtcgctgg agcggggcga ggcgcgggtg aatttcgatc ctgcccgcac
    44641 gggcgtcgcc gccttgcggg cggcggtcga agaggcgggt ttcgacgcgg cctgagggca
    44701 cggcggccgc atcgtgtgca ccgcgtgcgc ggccggtttc acatataatc ccgcggatta
    44761 attcaaacgg aggtaggtat gggttacgac caggtcaagg caggcaagga cgttccgaac
    44821 gacatcaacg tcatcatcga gatttccgcc cagggcgatc cgatcaagtt cgaggtggac
    44881 aaggacagcg gcgcggtttt cgtggatcgc ttcatgggca ccaccatgcg ctacccgatc
    44941 aactatggct acgtgcccca caccgtcgcc ggcgacggtg acccggtgga cgtgctggtc
    45001 gtcacaccct tcccgctgca gccgggtgtc gtcatccgtg cccgtcccgt gggcgtgctg
    45061 aagatggaag acgaaggcgg cgtggacgcc aaggtcgtcg ccgtgcccgt ctccaagctg
    45121 accccgctgt acgacaaggt ccagtccgtc gaagacctgc ccgagctgct gatgaagcag
    45181 accgtgcatt tcttcgagca ctacaaggat ctggagcccg gcaagtgggt caaggttacc
    45241 ggctggggca ccgtcgagga cgcccgccag gagatcctga acggcatcgc cgcggccgcc
    45301 aagtaagcgt ccggacctcc acggcttgaa aggcccgcgc aagcgggcct ttgtgtttat
    45361 gcgtgacgca ttacgtcaac ggggtgacga ttttcgtcac ccggacagcg cttgctgggc
    45421 ctcaagactg ccgtagaggg tgagggattt gtgttaagtg cttgttccgt aattatttat
    45481 tgtatttttt catattctgg catgtgcttt gcgttgcttt tcttgccggg ctgacttgcc
    45541 cgttcaattc gaacatcatc ctctggagct gagaatcatg accaagcaaa agcaaagcgg
    45601 ctttaccctg atcgaactga tgatcgtcgt ggcgatcatc ggcatcctgg cggccatcgc
    45661 cgtgccgcaa taccagagct acaccaagaa agcaaagttt actgaggtcg ttaacgcgac
    45721 ttcgtcccgc aaatcgttcg tggaagtgtg cctgcaggac ccgaatattg tggcggcgaa
    45781 ttgtgttggc ggctcgaacg gcattccgac tgatgacacg actggtttca acaacgttac
    45841 gtctgttacg gtgacgtccg gcggaaccat tacggcgact ggcaaggccg ccgcgtttgg
    45901 ctccagtggc aatatttacc agatgaccgc cacggtgacg aacggtaatg tggtgtggga
    45961 cgacaagggt tctggcagca cctgcaagac ggccggtctc tgctgatacg cagatcctcg
    46021 tgggcatgga caatggaccg cttcggcggt ccattgtcgt ttctgccgag ggtttcaaga
    46081 ttgttgctgt gagcagtgat atgaaagctt ggatccattt ttgcgtcagg gctcggattg
    46141 agactaagga tgggctgcat tcgtaatgcc cctggagcag aaagatatgt gtgaaattgc
    46201 acggcgcgca ttgaggttgc cggttttttc ctggctcgta cctgtgggat tcatgttgct
    46261 gtttttggca attgcgttgg gttctatttc tgggctgatc gccccactgg ggccttggct
    46321 caatgacgaa ttccgtcccc atgcttatcc ggcggttggc atcctggtac taacggttta
    46381 tgcgttttca gggccatcgt ggagagagct tggccttctg gtcttttcgg g
//
LOCUS       DAOQMM010000016        44702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_198_length_44702_cov_48.475329, whole genome shotgun sequence.
ACCESSION   DAOQMM010000016 DAOQMM010000000
VERSION     DAOQMM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 44702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44702
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198_length_44702_cov_48.475329"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="gap"
                     /locus_tag="PLH85_04545"
     CDS             complement(<1..995)
                     /gene="gap"
                     /locus_tag="PLH85_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNB63858.1"
                     /translation="MSIKVAINGFGRIGRCTLRAIYEQGLQNEFDVVAINASGDLATN
                     AHLLKYDTTHGRFATSVETEGDNVIIIDGKKIPFYSTKDPKGVNWAQHGVDVLLECTG
                     AYTTKAKAQALLEQGAQRVLISAPGGDDVDTTIVMGVNEGDLKAGYTVVSNASCTTNC
                     LAPVAKILADSIGIKQGLMTTIHAYTNDQVTVDVRHKDLRRARAAAANIIPTKTGAAK
                     AVGLVLPQLKGKVDGFALRVPTINVSLVDLTFTADRATTKEEINELMTAAANGPLKGI
                     LAVNTEPLVSSDFNHTTVSSTFDATQTRVIKGEDGSVLVKVLAWYDNEWGYSCRML"
     gene            complement(1056..3086)
                     /gene="tkt"
                     /locus_tag="PLH85_04550"
     CDS             complement(1056..3086)
                     /gene="tkt"
                     /locus_tag="PLH85_04550"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNB63859.1"
                     /translation="MSSTRNVKPPVFNPLTGAIRALAMDAVQKANSGHPGAPMGMAEI
                     AEVLWRRHLRHNPANPRWADRDRFVLSNGHGSMLIYALLHLTGYDLSIDDLKAFRQLH
                     SKTPGHPEVGYTPGVETTTGPLGQGITNAVGMALAEKILADTFNRPGHRVVDHHTYVF
                     LGDGCLMEGISHEACSLAGTWGLGKLIAFYDDNDISIDGHVGGWFTDDTPKRFEAYGW
                     HVVRDVQGHDPVEIEAALKAAQAETGRPSLICCKTVIGAGAPNKQGGHDVHGAPLGAA
                     EIEAARAYIGWTHPPFEIPADVYAAWDARTAGAAAEADWNTRFAVYAAEFPELAAEFT
                     RRMAGDLPADWAAHVDAVLAQVTAKGETIATRKASQNSIEAYAPKLPELLGGSADLAG
                     SNLTLWSGARGVTRSTGGNYVYYGVREFGMAAIANGVSLHGGLIPYTATFLMFSEYAR
                     NALRMAALMKARQIFVFTHDSIGLGEDGPTHQPVEQIATLRLIPNMDVWRPCDTTETA
                     VAWAAAVERRDGPTALAFSRQNLPFVPRSAGQIADIRRGGYVLSEAGTPSQPIAPQAI
                     IISTGSEVALALAAQKSLAADGIAVRVVSMPSTTAFDRQDAAYRCAVLPKGLPRVAVE
                     AGVTDIWRKYVGLEGEVIGLDRFGESAPAGELFKQFGITAEAVVGAVKQVIA"
     gene            3267..4652
                     /locus_tag="PLH85_04555"
     CDS             3267..4652
                     /locus_tag="PLH85_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63860.1"
                     /translation="MAAFPRGRRPLLALIAAALLAGGCSTTKYTVDDGRKVNEALLAN
                     LRLLGSGERALRPAIVRSAELKDPDCSHQWDLPVSVATSYEWPEDDRVAWVRALQVDE
                     RLTVVAAAPGSGFQVGDKIVEIDGYKREDSNKMLVELAEMRDNGKPFPVKTAAGRTVT
                     LKPVQVCRGYTRLAPPATPGYQDFHWLMSIHPLQVFIPPVTPDEALWMVLWTQGLSEE
                     GGARMKTYHYSKEIVSTLFEIASLATGLNAAAQAAKVAVNQAATAAATAAAKSASEAA
                     ARQALEQAARQISQQAAKDYAQKVGEEVAKSVGRQGVNVLRDSFMARMGMAVSSLSWV
                     ATTVFDDADRWAYDRIAKLGGDPLAGATLHQKLLDKGLIQNAFVLEEERLVRYAALAR
                     QTQREEMLAAVLKGASLEAFTLKLTDLPESSSEPAALEMASEMAEQGPGGTSGGLIDA
                     LLRMPLESGDD"
     gene            4672..>5681
                     /locus_tag="PLH85_04560"
     CDS             4672..>5681
                     /locus_tag="PLH85_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63861.1"
                     /translation="MTRLTPFAFAMLVGGSLAQAQSMPAADEPSAAAPADACPILLQG
                     AEAALERGRAGIAARAWKDAAAVLREASASFLKVAAGCPALAPQANRQGEKAVAELKL
                     AEGQMSHHSECQPRLDKALDLDIRAASVKSASGDPAEMERLLGESEALWREAVSLCLS
                     PHREKAERSLAATVRARAANAELLSAGPACDGAWKNATTLVDLAKAAWKDKRWDDAGM
                     LYSKAALAWEGAADKCSGNRQQLAQRKIEQTQTDAHNAEFCGPLWDAATEQSQRVKAA
                     GTSAALAERDQLSIRAEVAWRDAAAQCRGAPQNLARSNADALARERGAPLPPNAMAQY
                     GR"
     assembly_gap    5682..5781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5782..6605
                     /locus_tag="PLH85_04565"
     CDS             <5782..6605
                     /locus_tag="PLH85_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63862.1"
                     /translation="RERGAPLPPNAMAQYGRKLPVAGSAAAVAPASVPVAGAAGATAQ
                     VASLAVTPPLPTRPAPRDEPARPVVVAPPPAKPDTRSEPVAAKPAAAPAPAEPTVIVA
                     GDTTYRGAFVADQAAAGAVSGSGSVEWANGERYTGSLVGGRKQGRGRFDWVGGQWYEG
                     EWQDDRATGQGVIQFVGGNRYEGGVVDGEPQGRGTLIFASGDRYTGDFVRGVFHGQGT
                     YAWKNGNRYEGAWNLGRKHGPGRLMWAVGGGWEGEFRDDQKTDAGRDITVAAAPR"
     gene            complement(6621..7496)
                     /locus_tag="PLH85_04570"
     CDS             complement(6621..7496)
                     /locus_tag="PLH85_04570"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNB63863.1"
                     /translation="MSMKHPIIVVTGSSGAGTTTVRHTFEAIFWREKVNAAIIEGDSY
                     HRYTREEISALITDAENRGERGISHFGPEANLFEELENLFRTYGESGSGQRRHYLHSV
                     EEAARRGLKVGSFTPWEDLPPDTDCLFYEGLHGAVVTDKVNTCRHADLLIGVAPITNL
                     EWIQKIHRDTRTRGYSMEAVQDTILRRMHDYVHYIVPQFTHTHINFQRVPMVDTSNPF
                     IARDIPTADESMVIIRFRDPRGVDFPYLLRMIHDSFMSRANTIVVPGGKLDLAMQLII
                     TPLIWKLMERRRQWV"
     gene            complement(7585..8334)
                     /locus_tag="PLH85_04575"
     CDS             complement(7585..8334)
                     /locus_tag="PLH85_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HNB63864.1"
                     /translation="MARDVILPRYLRAVREHKADGSVLTEADLAAQAALEERLPHLIA
                     APVLGEEMTAEEQGRVWHAGKAGLWVIDPIDGTTNFANGIPFFGVAVAYLVDHKTQLG
                     VVYNPVTDEAFYAGRGAGAWLNDVSLPLRQPTERLKDAVAGVDFKRISHHLGDELAVR
                     PPYYSQRNFGSSALEWCYVAAGRLDVYIHGGQMLWDYAAGRLILEEAGGCYSSLGGTP
                     LTAGPAIKRSVISAASPALFEQWRTWLHAHA"
     gene            complement(8388..8951)
                     /locus_tag="PLH85_04580"
     CDS             complement(8388..8951)
                     /locus_tag="PLH85_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB63865.1"
                     /translation="MVSLSPPVCDFGWSAPDFALPGVDGRTHSLASCRGPAGLLVMFI
                     CNHCPYVKAILPRLLRDCRELAGLGVGSVALMANDPADYPEDSWEAMQRIAVEQDFPF
                     PYLLDASQDVARAYGAVCTPDFFGFNRDLKLQYRGRLDASGRQPAPSDTRRELFEAMA
                     QVARSGEGPRDQTASIGCSIKWRPSAP"
     gene            9003..9734
                     /locus_tag="PLH85_04585"
     CDS             9003..9734
                     /locus_tag="PLH85_04585"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNB63866.1"
                     /translation="MNPRFFCPDGLQPDSEMILPAAVAHHAERVLRLAVGEGVTLFDG
                     RGGECDAVLVALGRQPLARLGPRRAVERESPLAITLVQALAAGDKMDWVVQKAVELGV
                     AAIQPVAAERSVLKLAGERAAKRVAHWQQIVVSACEQSGRNRVPEVAEILPLARWLGT
                     PFAGRRLILAPGDAGALVRMAPPDGPLAVLVGPEGGWSPAELGLAMRAGCEPLALGPR
                     VFRSETAGLAALAALQTLWGDFPGG"
     gene            9736..11223
                     /gene="pap"
                     /locus_tag="PLH85_04590"
     CDS             9736..11223
                     /gene="pap"
                     /locus_tag="PLH85_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNB63867.1"
                     /translation="MFESAELGHKLSKAEYAEIEPVLRTELLETQLDLLDKKDFAAVV
                     LINGIDGAGRGETVNLLNEWMDPRHIEVHAFDRPTDEERERPFLWRFWRALPPRGKIG
                     ILFNNWYSEPLGDRAFGRIKKARLDQRLSEFNRFEAMLAAEGVVLVKFWFHLSRDGQK
                     KRLKALEANPATRWRVRASDRLVYDHYDRYRATAEHVLRRTSTAHSPWVIVDGSDPQY
                     RAVFVGRTLLHRLQAHLEHVRKGYRARLVSAPLVAAADRRTLLNALALDQPMERKAYE
                     AQREALQGRLSLALRRRGFTQRSLVLVFEGMDAAGKGGAIRRVTQALDTRQYHVIPIA
                     APSDEERAQPYLWRFWRRLPRRGRVAIFDRSWYGRVLVERVEGFCTEADWMRAYAEIN
                     DFEDQLVSHGALVVKFWLSISADEQLRRFKAREEAAFKRYKITPDDWRNREKWPLYEP
                     AVCDMIDRTSTENAPWTLVEADNKYFARLKILRTIVETVERSLKA"
     gene            11305..12657
                     /gene="argA"
                     /locus_tag="PLH85_04595"
     CDS             11305..12657
                     /gene="argA"
                     /locus_tag="PLH85_04595"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNB63868.1"
                     /translation="MTVPQSVPAAVPDPGLSQRFVAWVRAAAPYIHAFRGKTFVLGFG
                     GEVAAGSLAQTLAYDCNLLAALGVRLVLVHGARPQIEAELARRGLQPRYHKGLRVTDA
                     DAIECVKAAMAVTRIEVEALLSQGLPNTPMAGSFMRVTGGNFVTAKPVGVVDGVDYGF
                     TGAVRKVIAEEIEADLDQQNVVLMSPLGVSPAGEIFNMSMEAVAEAVAIALKAEKLIY
                     LCDAPGLLDEGGGLIEAITADDAEAMLKAGKGLTEDLDLYLPCAIRAVRKGVGRAHLI
                     DRDKDGGLLLEFFTPQGVGTVLTRDPLFRLREATIEDVGALGSMIAPMEADGTLVRRS
                     RERLEQEIERFTVVEYDGVLVGCAALYPFIDDRAAEMACVAVVPEHRRAGLGEMLLKR
                     IEARARALGVERLFVLTTRTSDWFRERGFRAMPPEELPRQKREMYDVERRSKVFMKAL
                     "
     gene            12672..14333
                     /locus_tag="PLH85_04600"
     CDS             12672..14333
                     /locus_tag="PLH85_04600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB63869.1"
                     /translation="MSAICSRICALRWRQVGAAVVPSLWAGGAMAAEAWPGGSLPALA
                     VVPALAAAALWACVLWRRIRALEATRLDPPVPVEPASRTGLPVASPAAGPWEHYFALA
                     ADSGHVLEGLIDTRGRLYWVSPAFERFAGHAVEDVLAAPDFVELIIYERDRRYVREQT
                     TQVLSTGRPADFETRVLTREGALRWVTCHWHAVRDEAGVVQGIRATGDDIQARKEAEF
                     KLLETVAALRRAQALSEHYLRRSNEERSRLSALLDVVEAGILFMDPDHRVVYCNRALC
                     EIWKVAEGESIVGVRDVVLQQHAVTLLTDPPGYLRHVAGVLGSREASAPFELHFSDGR
                     TVNEFSRVVFGDDGARAIGRIWVYEDVTERRRVALQLLNLAERDPLTNLYNRRRFHEE
                     LERMLGDARRRGDDVGLLVLDLDGFKPVNDAFGHQAGDQVLTGLAQAVAAVVRRNEMF
                     FRLGGDEFAILVPSADVQGLAGLARRVGACIAALRFDFGGSAVGVTASIGIAWSGLSG
                     TDAEALVGAADRAMYRAKAQGGNRSEVAAAAEGNDPEVGAEPGLG"
     gene            14394..14666
                     /locus_tag="PLH85_04605"
     CDS             14394..14666
                     /locus_tag="PLH85_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNB63870.1"
                     /translation="MVRMVNCVKLGREAEGLDMPPLPGDLGKRVYENVSKEGWQQWVR
                     HQTMLINENRLNLMDTRARKYLAEQMERYFFAGGADQIGGYVPPKP"
     gene            complement(14677..14997)
                     /locus_tag="PLH85_04610"
     CDS             complement(14677..14997)
                     /locus_tag="PLH85_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNB63871.1"
                     /translation="MNGPAQPEIPRAEAVALAYNAGNGAPSVVAKGRGLIAGEIIARA
                     REAGVYVHESPELVALLMQVDLDARIPPELYVAVAELLAWLYSLEDHARHQKETAPQG
                     RPLP"
     gene            complement(14994..16040)
                     /locus_tag="PLH85_04615"
     CDS             complement(14994..16040)
                     /locus_tag="PLH85_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNB63872.1"
                     /translation="MIPSDLAARLRMLTEASFFSGEPTVAPLARVREVPANLPDFLPG
                     QRIVARLQQSGPDNTFKALVEGRSVTLALPQTATPGDTLELVVSHVTPAAVFATQAGS
                     TALPGAQTGTALSQAGRLISFLLTGQPPAQAASLNGGQPVLASPPGPGDTDLLAQRLG
                     AALGDSGLFYESHQARWLAGQVDMAQLTREPQGRPAAPSAAPTTALQTGTAALPEAAQ
                     PGATTQIPDRLLPVVHQQLDALATQHLVWQGQIWPGQTMEWEIEDPARDGPRADDDPA
                     PTWNTTLRLSLPTLGGVEARLHLTPAGVALRLTGDSADTVAALRAGQPRLAEALAAAN
                     LPLTGVSTEQREPA"
     gene            complement(16095..16433)
                     /locus_tag="PLH85_04620"
     CDS             complement(16095..16433)
                     /locus_tag="PLH85_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNB63873.1"
                     /translation="MSSLALLEEMSTYSNEMVDAARHNDWDRLTRLEKKVASLRDRIG
                     VEEALGSPSRRRMSESERSRRIDLIRRILDDDKEIRVHTEPWLDNVRQMLTGQSRERA
                     VRAAYDKLDR"
     gene            complement(16541..16951)
                     /gene="fliS"
                     /locus_tag="PLH85_04625"
     CDS             complement(16541..16951)
                     /gene="fliS"
                     /locus_tag="PLH85_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNB63874.1"
                     /translation="MSFGLAARRADAYASVGVETGVSTADPHQLILMLFDGALLQVGA
                     AAAAMESGNVPGKGAAVSKAIEIITNGLKVSLDYEAGGELAARLGALYDYMTHRLLYA
                     NLHNSRPALDEVTKLLSELKSAWAEIGPGTDGSM"
     gene            complement(16991..18385)
                     /gene="fliD"
                     /locus_tag="PLH85_04630"
     CDS             complement(16991..18385)
                     /gene="fliD"
                     /locus_tag="PLH85_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNB63875.1"
                     /translation="MASISSAGLGSGLDVNSIVTSLMSVEKQPLTQLQTLQNKDQSKI
                     SAIGTLKSNLATLQSAAAALTPAIGQSPTAAFAAYTATLADTTIGSATATASAVAGTY
                     SVNVTSLATNQQLALGKTYASTDPVIDFGSASTKTLTFTVGSTATNVTLNSSANTLAG
                     VRDAINSANAGVGATIVTGTDGKQNLLLSATNGGTANAVTISGDASFIDASAPGSPIA
                     AASAFTQTQAATDAVVKIQGVSIATSGNTVTNAIDGVTLNLTKTGSTTLTVARNNSDL
                     QGKVTAFVNAYNSLNSSIKSLGAYDATTKSAAILNGDSSLRSIQSQVRAALTGTPTLS
                     SGNANIKLLSDIGVSFQRDGSLAFDTAKFATAASKDYVATATVIANYGSALKTTTTNL
                     LGTNGVIASRTNGLNASIKSIGNQIDSMNVRLTRIEANYRSQFTALDTAMSSMTTTST
                     YLTQQLTLLANQSK"
     gene            complement(18561..18947)
                     /locus_tag="PLH85_04635"
     CDS             complement(18561..18947)
                     /locus_tag="PLH85_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNB63876.1"
                     /translation="MSIQPVGGASTQPYTPPVQSTPGAQGAIRKADAPTAPGDVQAVR
                     DATQNPAAPPTPEQLNQAVKQIQDVIKQTAQSLQFSIDKDSGMTVVKVVDTESKKVIR
                     QIPSEEVMAIAKALDKLQGLLVKQQA"
     gene            complement(19029..19877)
                     /locus_tag="PLH85_04640"
     CDS             complement(19029..19877)
                     /locus_tag="PLH85_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNB63877.1"
                     /translation="MAATINTNSMSLNAQRNLSMSQSSLATSVQRLSSGLRVNTSKDD
                     AAGLAIADRMNAQAKGFDVAIRNANDGISMGQTADSAMQAITDNLQRMRELAVQSANG
                     TYTSGDRTNLDAEFQQLNQEIARVVSGTQFNGQNILTFTASFTFQVGAGTASTDQISV
                     TTAGSLASATFGTTTAGALSGSSISGSSSSGATAAISAIDAAIDSLNTSRATWGAVQN
                     RFSSVVQTLSVASENAKSSRGRIMDADFAQETANMTRAQILQQAGTAMVAQANSMPNN
                     VLSLLK"
     gene            complement(20172..23801)
                     /locus_tag="PLH85_04645"
     CDS             complement(20172..23801)
                     /locus_tag="PLH85_04645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB63878.1"
                     /translation="MRSLYPPPRHPYYIVAPDYRRTSAGIRVMHQLCHALRQSGEDAW
                     IQTGVTHPTLDTPRLTDEVRAAHRARGLTPIAIYPEVVAANPLDCAVVVRYILNRPGL
                     LGEQPAYGPDDIYYVYEPELLDIVPGATDVLRLPSINTSLFNNRDNPHDAHRSGSCIY
                     FGRYSAGQQEHPDLADTCTVITQDFPATHEELAELLRRSERVYCFENTAISTEARLCG
                     CPVVMLPSPFFSKEKFFGRGQGFDQGVAFTQSPEDLATAARTVGHIATLYESLEAAFW
                     PALDGLITLTQARADAAPKPDPTTRSSRRPTQDDTEYETWRARQTLQEIDAQILAERM
                     MLTWQQRPGIHLLLALHAGEEHLLADTLDSLAAQLYPEWRLTVATALDMPEGVGDISN
                     LQWLALRDAAHIDYVIDEMAAASPGTWLARIEPGLTLEPHALQVIADYINVRPDWRLI
                     YCDEDTLEADGRYTHPLFKPDFNLDLLRAQAYVGSLIVVEKQAFLAAGRFGTLAGAEN
                     YDLTLRILDHAGEAAIGHLDQLLAHLPRASVRAMQPEAEKAAVQAHLLRRGLDAEVLD
                     GAGYGTRRVICRWAESPLVSIIIPTRDKVEYLRPFIESLEERTRYRPYELIIVDNDSV
                     DPDTLDYLAALAGRHARVRTRILRQPGEFSWSACANAGAAVAEGDFLLFLDNDLHIVQ
                     DDWLDRLVAIAQRPEVGIVAPRLTYPETAKVQQGVWILGMHGTTGNPWDNQLGLTDPG
                     YMGRALCDQNVSAASGSALLVKRAVLTSLNGFDAERFPLLYGALDLCLRATAQGHKIV
                     WTPYAALVHYAGASVVARQRRLEGKLTDLIAAQEANQTLLARWMPRLVSDPAYNRHLS
                     LSFPFKPEHVAPMDWDPHFHDRPRILACPVPGGAGEFRLRAPLRVIGQAGLAQTMICE
                     PPKPFTMRILSPVEVARADPDVLILHQPLEDTQSDALEHYARFLPKVRRILTMDDLVT
                     NLPRKHPLYKSGYKDGRQRLRRVLSFMDRLVVSTQPLADLCADMIDDILVMPNSLEWS
                     LWGDVAPPGRPRTKPRVGWAGAQQHQGDLELIYKVVEALADEVDWIFMGMCPEPIRGF
                     VHEAHGFELDFRAYPRALSRLDLDLAIAPLDVHPFNEAKSNLRLLEYGAMGWPVICTD
                     IYPYQNAPVTRLPNDPDLWIRTIREQIAEPAALKAAGLALQRWVREGYVLENHTARWF
                     AAYGP"
     gene            complement(23860..24654)
                     /locus_tag="PLH85_04650"
     CDS             complement(23860..24654)
                     /locus_tag="PLH85_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63879.1"
                     /translation="MNTATLAPAAFDDFGRCLPLGVSAPANARSRRYFRLGTPALDPA
                     AIHARLARHLGAGKDMSTDTFIQRIAALRVRLAADPATAGLLDGPAVPFILPRTPVTE
                     IGTLLEEGYLPAVARSFEEAFPNARFTDHAHQGLGGKLSVQPGSRHEILVDALAHGEV
                     VGLYFPCLTEYSLPAAVERMAGLPEHLLLAGGFDTAAAFIAAPDLLLRTDGYPPLLWL
                     AGLQGEKPGIGYHFEAYGYNLTFNRRPHLDQTAEYWWSGVTVLAPA"
     gene            complement(24651..25766)
                     /locus_tag="PLH85_04655"
     CDS             complement(24651..25766)
                     /locus_tag="PLH85_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB63880.1"
                     /translation="MTTALIPDSGLTAAEAAKRNDLATRKPYVAAKLARIAEMEERGE
                     ISPIIRLEKSYLCNFQCTHCSAEYYMDRHLEKVFKIKDERQKIDLDDIRRLSKEADAL
                     GLARFVITGGEPLVMKDFDAVVEAIDPERHYVITDTNGWFLDLARARHIKSVGVEKVQ
                     LSLDSMVEAEHDAFRNKPGSYKRVMRAIDAAQEAGLNLILSTVLVRGRARTDEFRQMC
                     DFAKARGIGLYVSYAKPTGSCTNHPEFVIDKEDADIVRELEKTHPVFTHMTPSYGSFK
                     GCITVKGIITVTSTAEVTPCPYIDFSLGNLRQTPLKEILDRGMRNPWLGPHRPDCLIG
                     EDPQFIRIHTERTKGAVTLPLPWGQGFSDHDSLQDEQ"
     gene            complement(26007..27080)
                     /locus_tag="PLH85_04660"
     CDS             complement(26007..27080)
                     /locus_tag="PLH85_04660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB63881.1"
                     /translation="MTASQRFTAEIDRAVVRFGDGWTALRGARLFITGGTGYFGRWLL
                     PALIRADAVHGLGLTATVLSRDPARFLADAPEVAASPAVRFHPGDVRDFAFPDGDFTH
                     AIHGAATSAAATFHRREDALTKFDTTLQGARRLLDFATERRIPRLLMLGSGAFYGPLP
                     ADRAAYAEDLPVAPSPDNLDVAIGHAKRAAEFLSAAYAERHGLSISTARCFSFVGPHL
                     PLDLHYAIGNFIRDALRGEAITVGGDGTPVRAYLYAGDLVVWLLTLLVRGAPGRAYNV
                     GSDQAVTIADLAQRVARVVAPAVPVRVLGTTTDVATRNVYVPSIERARGELGLDVWTP
                     LDEAIRLTADVARAGAAPTQARN"
     gene            complement(27087..28232)
                     /locus_tag="PLH85_04665"
     CDS             complement(27087..28232)
                     /locus_tag="PLH85_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNB63882.1"
                     /translation="MNAPDSPLRRLLHTPPATPAAPCRVADVLAGRPVVVYGCGDGLI
                     TFSVFVLDKFGIRPEVCLDAKFTAPTTLGDIPAMAPADYRPDPALLDRAVAVVTVGKT
                     RFHADIFATLRGLGFTRIVLASDLYEYHLSHAPAGFEQLGQTWFAERADVIERAYALL
                     ADAKSRSVFEAVLQTHVAQMPVPIPRDPLEHQYFPHDVPLAAGVARTLNCGAYDGDTV
                     RQLHARHGRIEALACFEPDLPNFTRLRATLTEHAGQLAGTVMAFPCGVFDDDVQLRFA
                     GGNRINSTLSDDGDTVVQCVALDHVLPDFRPTFINMDIEGAEPHALRGARQLIRANRP
                     DLAICVYHHPAHLWEIALQIHALVPDYRFYLRNYTGFPAETVLYASR"
     gene            complement(28242..29768)
                     /locus_tag="PLH85_04670"
     CDS             complement(28242..29768)
                     /locus_tag="PLH85_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63883.1"
                     /translation="MARLALISLHDTTGLVDDARRLIGAGFTLLASRESSSLIAAAGL
                     PVIDLADYTALTEDFGFPPTLHPKLERALTARPADLAEPAIELAYINPYPADVGNDVG
                     GRTILMLAVKGQRLAASSRADLHRVLACLETGGDLPALRRQLADAACFSVARHFAGLI
                     SPDAPFATSFGEAWLDLAEGENPYQRPAVAYRSGDPDPLALSRFRLLGSQPPCFTNAA
                     DADAILHHLCLLAEGFEKNTGTVPWLCIAAKHGNACGIGVSRHAPREAIDRALAGNPR
                     AIWGGEVIVNFPIDGELAAALFASDEREVRLGSRHWMLDVVMAPAFTAEAAELLGRRE
                     FRKLLANPALAAARLPHGRRMLRPVRGGALGQPVADYVLELAACAGNARFDEAAVTDL
                     LIAWSAAYSSNHGGNEVALARGGMLLAAGGGPSTVDAARSAVTRCAEQGHDATGAAFA
                     ADAFFPFTDAPQIVADAGARCGAVPDGSRNDDTVKAFFRERHIAVAWIPAAFRGFCRH
                     "
     gene            complement(29771..30325)
                     /locus_tag="PLH85_04675"
     CDS             complement(29771..30325)
                     /locus_tag="PLH85_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63884.1"
                     /translation="MSCDLCIKHLPGETDCVWHREHKEHGKDYGPHNILPSNVCPIMY
                     HTLYPYFLGALFGARYAYNEQGDCQVCCPAAKGVDVLVKVRPNDGKFEAGVDADWRDV
                     IHAEVVAVHGDCPYQHKVGDRIVFPTTRSMRTQYMCPAGFNNLFPFLKIDIPSCIDFD
                     RVRCPDWMENIYYRIDENSRVRKD"
     gene            complement(30322..31431)
                     /locus_tag="PLH85_04680"
     CDS             complement(30322..31431)
                     /locus_tag="PLH85_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNB63885.1"
                     /translation="MSYKIHMDGYAEPRTVFQIEALHAWKHKQYFAPVIVEVSPVGYC
                     NQKCKFCYSDKIETKGDKIPDAILTRLFCDLAGMGVQAVSLQGTGEPLMHGGLPDAIV
                     AGAGKGLRYSLTTTGVVFTEKIQSRILQHLTFMKCSVLENDRDRYALMHGCSPKQWDQ
                     LIANLGAAANRRSREGLDVALMGTVYVELNNFDRLPQIIRFYRDLGLDYIVVQEATYT
                     EFTPSNQSRNTSEHFTAAEIDAMKAEVLKLNTEDFTVKVRFPVDDTSYHVGMYKDCWK
                     DDHCQGIKFFPLVAADGNVYSCYRAWGKSEFSMGSLHTQSFEEIWRGERRQEVERQIM
                     GTPPGGDECAVCGISKLNDILSKYEQPNKWRSFLI"
     gene            complement(31445..32608)
                     /locus_tag="PLH85_04685"
     CDS             complement(31445..32608)
                     /locus_tag="PLH85_04685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNB63886.1"
                     /translation="MINSLYKNRTAIYFGTGMEDAGLTELARLGARHVLLHYGQGSVT
                     RSGLLDRVMAGLTARQIAVTELGGVQPNPTLPRVREGVALCRARGIDTLLAVGGGSVI
                     DSAKAIAAGAAFDGDVLDLIEGRVRPAAALPLGVILTVPGAGSESSPAAVISDPARSL
                     KLDFAAECLVPALAVLNPENTRGVGLFQSRCGILDAITHVCERYFTQTDYVEVSDRLC
                     EGLIQSLIHYGRAVDGRLDDYGVRAEIMWACKLAHDDVAGVGRKQDWSSHVIANALGG
                     HVDQPHGALTVIVMLGWMKRAAETSPARFVQFGQRIFGLASPTAAEAIAAFEHYIAGI
                     GMPVRLSGLGVDLAPATLDAVVDVCARKNPSGTIGNFVRLGPNDVRTILETAR"
     gene            complement(32605..33207)
                     /locus_tag="PLH85_04690"
     CDS             complement(32605..33207)
                     /locus_tag="PLH85_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB63887.1"
                     /translation="MTTPSPDALREAIATLRAALGPAPRNGLPEDLFLFVSSLTPLVN
                     VDLLIRDDAGRMLLGWRHDAFYGPGWHVPGGIIRFREDAATRIAAVAARELGARVRFE
                     PEPLCIHEIVNDVREERGHFISMLYACTLDTPPDERLRFDPSAPVDGAWAWHAACPDP
                     LIDAHDLYRPWLGPRGAPRPTRRSGRQPQRHWHLLSGRPA"
     gene            complement(33204..34346)
                     /locus_tag="PLH85_04695"
     CDS             complement(33204..34346)
                     /locus_tag="PLH85_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB63888.1"
                     /translation="MSTTCRLCGHPLFPEPLLVQHAMPAAAQGLPTAEQLADDQGVTL
                     ALHQCAGCATVQLANPPVPYWREVIRAAGLSAEMRAFRREQFGRWIAEHDLTGRKVLE
                     VGCGRGEYLALLAEAGADAHGLEYAPAAVAACHAAGLHAHRGFLEDPSIRLASGPFDG
                     FAILNFLEHIPAAPLTLQAIAANLTDGASGLVEVPNFDMILRAGLFAEFIPDHLFYFT
                     RQTLTALLERNGFDVLDCRPAWHDYVLSATVRKRRPLDLSPLLACQDALKAAFDAFLA
                     RFAPGRVAIWGAGHQALALISLMALAPRVAYVVDSAPFKQGRYTPATHRPIVAPERLA
                     DGEVDAVIVLAASYSDEVARILRERHGSRFTVAVLRDNRLDTVIAP"
     gene            complement(34351..36183)
                     /locus_tag="PLH85_04700"
     CDS             complement(34351..36183)
                     /locus_tag="PLH85_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNB63889.1"
                     /translation="MPTLRVADWIMGRLADLGVDHVFLLPGGGAMYLDDGLACEPRLT
                     AVPCHHEQAAAIAAEACGRTGAAGNAGFGVCVVTTGPGATNAITPVAGAWIDSVPLLV
                     ISGQAKRPDRLNGRPLRQGGVQEVDVVPMVRTITKYAVTVEAPQAIRAHLEEALFRMQ
                     DGRPGPVWLDIPLDVQAAPVDPDTLPGWTPPAAELPPAPDFATLADLLARAERPLLLA
                     GHGVRLAGAAEAFRQFTERLQIPAVLTWNALDLLPYDHPANIGRPGVVAARAANFAVQ
                     NCDLLVSVGCRLDNIVTAYNPAGFARSAVKVVVDVDPHELAKLPMPVEHPLAVDARDF
                     LDGLLAASADLPPLPCDAWRTRCASWKARYTQNEGRVFPPAGPIGHAHFVEALSDAAP
                     PDTLIATGSSGLAIEFFYAGFRNKAGQRSFLTSGLGAMGYGLPAAIGACLGNGRRPML
                     AVESDGSLQLNVQELATLAGQCLPVCLFILNNGGYASIRNTQRNYFQGRYLGTGPEGG
                     LLMPDLEKLAAVYDLPYLRIDDCAGLADALGRAQALPRPCLIDVHLVPDESLAPKCAA
                     IPRADGSIVSMPLEDMSPLLPLDVLKAEMIVPLLPASLDAPRPA"
     gene            complement(36189..37685)
                     /locus_tag="PLH85_04705"
     CDS             complement(36189..37685)
                     /locus_tag="PLH85_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNB63890.1"
                     /translation="MNKPLMVFEMANNHMGDLPHGLATIRALREACTGFEDVFDFAFK
                     LQYRDLDTFIHASARGRDDIKYVKRFEETRLSDEAFRALVAEMDACGFRTVCTPFDEP
                     SVDRIEAHGIHTLKIASCSFTDWPLLERMATSAKPVIASTAGATLEEIDNVTSFFLHR
                     GKDFTLMHCVAEYPTPDASLQVNQIELLRSRYPGVRIGYSTHEAPDNTLAVGLALAKG
                     ASVFEKHVVLPTERYAANAYSANPAQIRAWLSEARRALAICGTSGDRYVPSAAERASL
                     QSLRRGVFATCAIPAGTELDATMVEFAFPPVDGQLTANDWSKYARFVTTAPIAAGQPV
                     LQAQVAEHQLRGKVLEIVTAVKALLKAGNVVVPGQSDLEISHHYGLELFHEFGLTMVT
                     VVNREYCKKLLVMLPGQLHPEQYHLKKEETFVVLHGALILWLDGEERHARAGDVITVA
                     RGVRHTFRTEEGAVFEEISSTHDRNDSYYTDPAIAQNTHRKTWLTYWM"
     gene            complement(37682..39097)
                     /gene="rfbH"
                     /locus_tag="PLH85_04710"
     CDS             complement(37682..39097)
                     /gene="rfbH"
                     /locus_tag="PLH85_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNB63891.1"
                     /translation="MSVPQTRLAVTDPLAAATALQRRRVQADAAGRIALNLPELAGQD
                     LEVIVRPARAPAEPSAWRPDLDPVPPSGKVIGDAERNAMIDAALDGWLTTGRFNDAFE
                     ARLAAFVGSRYALTVNSGSSANLVAFSALTSPRLGERAIRPGDEVITVAAGFPTTVNP
                     ILQNGAVPVFVDAALPGYNLDVTMLEAALSPRTKAIMIAHTLGNPFDLDAVCAFAERH
                     GLWLVEDCCDALGSTWKGRRVGTFGHLATLSFYPAHHITMGEGGAVLTSDPQLRQIAE
                     SFRDWGRDCWCKPGCDATCGKRFGWQLGSLPTGYDHKYTYSHIGYNLKITDMQAACGL
                     AQLDRLDAFIAARKRNFAYLNAALADAADVLILPEATPGSDPAWFGFPLTLRPEAGVH
                     RVDLLQYLDQHRIGTRLLFAGNLIRQPSMAGRTFRVVGDLPVADRILHDTFWVGVWPG
                     LDTAQLDHIARHIRCFLGVDL"
     gene            complement(39094..40161)
                     /gene="rfbG"
                     /locus_tag="PLH85_04715"
     CDS             complement(39094..40161)
                     /gene="rfbG"
                     /locus_tag="PLH85_04715"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNB63892.1"
                     /translation="MENLVNPEFWRGRRVFITGHTGFKGSWLSLWLQALGAEVHGYAL
                     APDTEPALFNVAHIARGMQSTLGDLRDLPALCAALHAARPEIVLHLAAQPLVPRSYAE
                     PVETFAINALGTAHLLEAVRLQPGVQAVVVVSSDKCYENHESLWGHREDAPLGGHDPY
                     SASKACTELIAAAYRRSFLTPRGVALATARAGNVIGGGDWTPTRLVPDILAAFAAGAA
                     VTLRNPEAVRPWQHVLEPLAGYLLLAEKLVLDGLPWAEGWNFGPDDTDSRSVAWIVER
                     LAAGWGDHARWRVADGPRIHETQALRLDCTKARRRLGWHPRWSAETAVDRSLAWYRAW
                     RTGADMRRACLDEITAFGTRP"
     gene            complement(40146..40919)
                     /gene="rfbF"
                     /locus_tag="PLH85_04720"
     CDS             complement(40146..40919)
                     /gene="rfbF"
                     /locus_tag="PLH85_04720"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNB63893.1"
                     /translation="MKAVILAGGLGTRISEETVSRPKPMITVGGRPILWHIMKIYSSH
                     GIHDFVICCGYMGYVIKEYFANYFLHMSDVTFDMTHNRMEIHERHAEPWRVTLVDTGE
                     STQTGGRLARVRQYVQDEDAFCFTYGDGVADIDIGAEIAFHREHGKLATVAAVQPPGR
                     FGALKIRAGGCVSAFVEKPAGDGGFINGGFFVLSPKVLDAIDGDQTTFEGPVLASLIA
                     RKQLAAFPHTGFWRPMDTLRDKTVLEELWTSGNAPWKTW"
     gene            complement(41044..42117)
                     /locus_tag="PLH85_04725"
     CDS             complement(41044..42117)
                     /locus_tag="PLH85_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB63894.1"
                     /translation="MRIQSLHPFLSRPVSRHRAPPAPRPTLRALAGTALVALTALSLP
                     AQASCFWSTAKSYSGAVGLLVGASGLIRTPTGEAVRVGVTAGTAVGELIDYGISHWWD
                     PSLELVPDDPSQVVTDPAQSLIQSAITSLLSDFRGSAPYATTWADLQALNATEAGFGD
                     AMTTMLSSGVSAFVHIAQGEAAYTAFGRGSAQVLAAAARVNDDLQRFSAAQTDLVPYL
                     APFDGTPGFPTVTKASYQSFLDGILSRGAAGLPPGEVSAGQRLLSLAGVHFVPGKALD
                     SSLVTYLSAGDTGNEMAQIPDAGIGLSGLIAARAPGFAGDCCTCGDFCLPEPASVALV
                     LTGVTGLGLQRRRRAIDDLPSAA"
     gene            complement(42312..42971)
                     /locus_tag="PLH85_04730"
     CDS             complement(42312..42971)
                     /locus_tag="PLH85_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNB63895.1"
                     /translation="MTAPADPLAAAHAAYTPLSLRLYDAVVMGVSNPLLWKCPTARLR
                     AHFDRHVSGNHLDIGVGTGYFLDHARFPVHAPRIVLMDLSTAALAHAARRLARYRPET
                     VIGNVLAPLPVPGRRFDSASLNYVLHCLPGPFAAKLSAFDHIRPLLNPGAVLFGSTLL
                     QGDAPRNAAARSLMAWYNRKGIFSNAGDDFATLQAALRQRFRQVALERVGCAALFAVR
                     N"
     gene            43158..43616
                     /locus_tag="PLH85_04735"
     CDS             43158..43616
                     /locus_tag="PLH85_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63896.1"
                     /translation="MSSSELPVRFSAAEMEQIEAALTTLERLFMRYYILDSKERYGMG
                     ELGSTGEALCRRMLKIFAHEPLIMPDDFDLLAFRRDLAALDQLRPILARLQTLTDKAT
                     DAEALLNDELSFSVFDGYALVKMIGRPEDFDALLAIMAGTATEDFSGPPL"
     gene            complement(43632..44114)
                     /locus_tag="PLH85_04740"
     CDS             complement(43632..44114)
                     /locus_tag="PLH85_04740"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNB63897.1"
                     /translation="MTDTADGAAATAGILAPPPLLYLGAFFAGSLAEALWPTASWAMT
                     GWLRGAGLVLFIASAAFARWAFVTLRRMGTSASPRQASAALGTDGPFGWSRNPIYVAM
                     TGLYLGLTGLLASPWPLLPLAPLLVAMHWGVIRREERYLHARFGAAYVDYRRRVRRYL
                     "
     gene            complement(44121..>44702)
                     /locus_tag="PLH85_04745"
     CDS             complement(44121..>44702)
                     /locus_tag="PLH85_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNB63898.1"
                     /translation="PAILASFREAFARAGVAPVRAIDASVIGPPLVETLQLLAGRTDA
                     ALIDTLAGHFKASYDTTGYQATAAYAGVGEMLARLKGAGRYLAIATNKRLHPTGLILA
                     HLGWDGYFDAVYALDRFTPRLPDKAAMIARLVADEGVPRDGAVYIGDRSEDGESADAN
                     GLPFIATTWGYGSLTPADLKPHWRAVNTPAELG"
BASE COUNT         6596 a        13387 c        17363 g         7256 t
ORIGIN      
        1 agcatgcggc aggagtagcc ccactcgttg tcgtaccagg ccagcacctt gaccagcacg
       61 gagccgtctt cgcccttgat gacgcgggtc tgggtggcgt cgaaggtgga ggagacggtg
      121 gtgtggttga agtcggagga caccagcggc tcggtgttca ccgccaggat gcccttgagg
      181 gggccgttgg cggcggcggt catcagctcg ttgatctctt ccttggtggt cgcgcgatcg
      241 gcggtgaagg tcaggtccac cagggagacg ttgatggtcg gcacgcgcag ggcgaagccg
      301 tccaccttgc ccttgagctg gggcagcacc agcccgacgg ccttggcggc gccggtcttg
      361 gtggggatga tgttggcggc cgcggcgcgg gcacggcgca gatccttgtg gcggacgtcc
      421 acggtcacct gatcgttggt gtaggcgtgg atggtggtca tcagaccctg cttgatgccg
      481 atgctgtccg ccaggatctt ggctacaggg gccagacagt tggtggtgca ggaggcgttg
      541 gagacgacgg tgtagccggc cttcaggtcg ccttcgttca cgcccatgac gatggtggtg
      601 tccacgtcgt cgccgccggg ggcggagatc agcacgcgct gggcgccttg ctccagcagg
      661 gcctgggcct tggccttggt ggtgtaggcg ccggtgcatt cgagcagcac gtctacgccg
      721 tgctgggccc agttgacgcc cttgggatcc ttggtggagt agaaggggat cttcttgccg
      781 tcgatgatga tgacgttgtc gccttcggtc tccacggagg tggcaaagcg gccgtgggtg
      841 gtgtcgtact tcagcaggtg cgcgttggtg gccagatcgc cggaggcgtt gatcgccacc
      901 acgtcgaatt cattttgcag gccctgttcg tagatggcgc gcagcgtgca gcggccgatg
      961 cgaccgaaac cgttgatggc gaccttgatg ctcatcgagt tttctcctgg ttggaatgct
     1021 ggtaacgata atccctgccc gccgcgcgct gcgcgtcagg cgatgacttg ctttactgcg
     1081 ccgaccaccg cttcggcggt aatgccaaac tgcttgaaca gctcgccggc cggggccgat
     1141 tcgccgaagc ggtcgagccc gatgacttcg ccctcaaggc ccacgtactt gcgccagatg
     1201 tcggtgacgc cagcctcgac ggccacgcgg ggcaggcctt tgggcagcac ggcgcagcgg
     1261 taggcggcgt cctggcggtc gaaggcggtg gtgctgggca tggagaccac acgcaccgca
     1321 atgccgtctg cggccaggga cttctgggcg gcgagggcga gggcgacctc ggaaccggtc
     1381 gatatgataa tggcctgcgg cgcaatcggc tgtgacggag ttcctgcctc cgacagaaca
     1441 tagccgccgc gacggatgtc ggcgatctgt ccggccgagc gcggcacgaa gggcaggttc
     1501 tggcgcgaga aggccagggc cgtggggcca tcgcggcgtt cgacggcggc ggcccaggcc
     1561 acagcggtct cagtggtgtc gcaggggcgc cagacgtcca tgttggggat caggcgcagg
     1621 gtggcgatct gctcgaccgg ctggtgggtg gggccatcct cgccgaggcc gatggagtcg
     1681 tgggtgaata cgaagatctg ccgcgccttc atcagcgccg ccatgcgcag ggcattgcgg
     1741 gcgtattcgc tgaacatcag gaaggtggcg gtgtagggga tcaggccgcc gtgcagggac
     1801 acgccgttgg cgatggccgc catgccgaat tcgcgcacgc cgtagtacac atagttgccg
     1861 cccgtgctac gggtgacgcc cctggcgccg gaccacaggg tgaggttgga gccggccagg
     1921 tcggcggagc cgccgaggag ttccggcaac ttgggtgcgt aggcttcgat gctgttctgg
     1981 ctggccttgc gggtggcgat ggtctcgccc ttggcggtga cctgggcgag gaccgcgtcc
     2041 acgtgggccg cccagtcggc gggcaggtcg ccggccatgc ggcgggtgaa ttcggcggcg
     2101 agctcgggga attcggcggc gtagacggcg aagcgggtgt tccagtcggc ttcggcggcg
     2161 gcgcctgcgg tacgggcatc ccaggccgca taaacatcgg ccgggatctc gaagggcgga
     2221 tgggtccagc cgatgtaggc gcgggccgcc tcgatctcgg cggcgccgag cggcgcgccg
     2281 tggacgtcgt gcccgccctg cttgttgggg gcgccggcgc cgatcacggt cttgcagcag
     2341 atcaggctgg ggcggccggt ttcggcctgg gcggccttca gggcggcttc aatctcgacc
     2401 gggtcgtggc cctggacgtc gcgcaccacg tgccagccgt aggcttcgaa gcgcttcggg
     2461 gtgtcgtcgg tgaaccagcc gcccacgtgg ccgtcgatgg agatgtcgtt gtcgtcgtag
     2521 aaggcgatca gcttgcccag accccaggtg ccggcgaggg aacaagcttc gtgggagatg
     2581 ccttccatca ggcagccgtc accgaggaac acgtaggtgt ggtggtcgac gacacggtgg
     2641 ccgggccggt tgaaggtgtc ggcgaggatc ttttcggcca gcgccatgcc cacggcgttg
     2701 gtgatgccct ggccgagggg gccggtggtg gtctcgacgc ccggggtgta gccgacttcc
     2761 gggtggcccg gggtcttgct gtggagctgg cggaaggcct tgaggtcgtc gatggacaga
     2821 tcgtagccgg tgaggtgcag cagggcatag atcagcatcg aaccgtggcc gttggacagc
     2881 acgaagcggt cacgatcggc ccagcgggga ttggcggggt tgtggcgcag atgtcggcgc
     2941 cacaggactt cggcgatctc cgccatgccc atcggggcgc cggggtgccc ggaattggcc
     3001 ttctggacgg cgtccatggc cagcgcacga atcgcgccgg tcagagggtt gaagacaggc
     3061 ggcttgacgt tacgcgtaga agacatcgac tggaccttcg gccggcgggg cggcgcgcaa
     3121 agaccgcaat tatcgtggat tttcctgtac ttggatagag gctgcaggcc gtcgcccggg
     3181 cgtgcggcgg cgcacgatgg cgaaatggaa aatacggcat aatccgggcg ccccttacat
     3241 ttccttactt ttgtgatctt cgcgcgatgg cagcttttcc gcgtggccgg cgaccgctcc
     3301 tggccctgat cgccgccgcg ttgctggcag gcggttgcag cacgacgaaa tacaccgtcg
     3361 atgacgggcg caaggtcaac gaggcgctcc tggccaacct gcgtctgctg ggcagcggcg
     3421 agcgcgccct gcgtccggcc atcgtgcgct ccgccgagtt gaaggacccg gactgcagcc
     3481 accagtggga tctgcccgtc agcgtggcca ccagctatga atggccggag gatgaccggg
     3541 tagcctgggt gcgggcactg caggtggacg agcgcctcac ggtggtggcg gcggcgccgg
     3601 gctcaggttt ccaggtgggt gacaagatcg tcgagatcga cggctacaag cgcgaggaca
     3661 gcaacaagat gctggtcgag ctggccgaga tgcgcgacaa cggcaagccc tttccggtga
     3721 agacggcggc gggccggacg gtcaccctca aacccgtgca ggtgtgccgc ggctacaccc
     3781 gcctcgcgcc gcccgccacg cccggctatc aggatttcca ctggctgatg agcatccacc
     3841 cgctgcaggt gttcatcccg ccggtcacgc cggacgaggc cctgtggatg gtgttgtgga
     3901 ctcaggggct gtccgaagag ggcggcgcgc ggatgaagac ctatcactac agcaaggaga
     3961 tcgtgtccac gctgttcgag atcgcgtccc tcgccaccgg cctcaatgcg gcggcccagg
     4021 cggccaaggt ggcggtcaat caagcggcca ccgctgccgc gacagcggct gccaagtcgg
     4081 cgagtgaagc cgcagccaga caggcgctgg agcaggcggc ccggcagatc agtcagcagg
     4141 ccgccaagga ctatgcccag aaggtcggcg aggaggtggc caagtccgtg ggcagacagg
     4201 gcgtcaacgt gctgcgcgac agtttcatgg cacgcatggg catggcggtg tcgagcctgt
     4261 cctgggtggc gaccacggtg ttcgacgacg ccgaccgctg ggcctatgac cggatcgcca
     4321 agctgggcgg cgaccccctg gccggcgcca cgctgcatca gaagctgctg gacaaggggc
     4381 tgatccagaa cgccttcgtg cttgaagagg aacgcctggt gcgctacgcc gcactggcac
     4441 gtcagaccca gcgtgaagag atgctcgcgg cggtgctcaa gggtgcgtcg ctggaggcgt
     4501 ttacgttgaa gctgaccgat ctgcccgagt cctcctcgga gcccgcagcc ctcgaaatgg
     4561 cctccgaaat ggccgagcag gggcccggcg gcacttcggg cggactgatc gatgccctgc
     4621 tgcgcatgcc cctcgaatcc ggggacgatt gacggaactg ccatgccgct gatgacccgc
     4681 cttacccctt tcgccttcgc aatgctcgtg ggcggatccc ttgcccaggc tcaatccatg
     4741 ccggcggccg atgagccgtc tgcggcggcg ccggccgacg cgtgtccgat cctgttgcag
     4801 ggggccgaag cggcgctgga gcgcgggcgc gccggcatcg cggccagggc ctggaaggac
     4861 gcggcagcgg tgctgcggga ggcctctgcc agcttcctca aggtagccgc cggctgcccg
     4921 gccctggcgc cccaggccaa ccggcagggc gaaaaggcgg tggcggagct caagctggcg
     4981 gaagggcaga tgtcccacca cagcgaatgt cagccgcgcc tcgacaaggc cctcgacctg
     5041 gacatccgcg ccgcatcggt gaagtctgcg agcggcgatc cggccgagat ggaacggctg
     5101 ctcggcgaat ccgaagccct gtggcgtgag gcggtcagcc tgtgcctgtc gccccaccgg
     5161 gaaaaggcgg agcgcagcct ggccgccacg gtgcgtgccc gcgccgccaa cgccgagctg
     5221 ctcagcgccg ggccggcctg cgatggggcg tggaagaacg ccacgaccct ggtggacctg
     5281 gccaaggcgg cgtggaagga caagcgctgg gacgatgcgg gcatgctcta cagcaaggct
     5341 gcgctggcct gggagggggc cgccgacaag tgttccggca accgtcagca actggcccag
     5401 cgcaagatcg aacagactca gaccgacgcc cacaacgcgg agttctgcgg ccccctgtgg
     5461 gacgccgcga ccgagcagtc ccagcgcgtc aaggccgccg ggaccagcgc cgccctggcc
     5521 gagcgggacc agctctcgat ccgggccgag gtggcctggc gcgacgctgc ggcccagtgc
     5581 cgcggcgcgc cgcagaatct cgcccgttcc aacgccgatg ccctggcgcg ggagcgcggc
     5641 gcgccgctgc cccccaatgc gatggcccag tacgggcgca annnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn ncgcgggagc gcggcgcgcc gctgcccccc aatgcgatgg
     5821 cccagtacgg gcgcaagctg ccggtagccg ggagcgctgc ggccgtcgca ccggcgtcgg
     5881 tgcccgtggc gggtgcggcg ggggcgactg cgcaggtcgc gagcctcgcc gtaacgccgc
     5941 cgctgcccac gcgcccggca cccagggacg agcccgctcg gcccgtcgtc gtggcgccgc
     6001 cacccgcgaa gcccgacacc cgcagcgagc ccgtggcggc gaagccggcc gctgcgccgg
     6061 cgccggccga gcccaccgtg atcgtggcgg gcgataccac ctaccggggc gccttcgtcg
     6121 ccgatcaggc ggccgctggc gcggtgtccg gcagtggctc ggtggagtgg gccaacggcg
     6181 agcgttacac cggctccctg gtgggcggac gcaagcaggg tcgcgggcgc ttcgactggg
     6241 tcggcggcca gtggtacgaa ggggagtggc aggacgatcg ggccaccggt cagggcgtga
     6301 tccagttcgt cggcggcaac cgctacgaag gcggcgtggt ggatggggag ccccaggggc
     6361 gcggcacact gatcttcgcg tcgggcgacc gctacaccgg cgacttcgtg cgcggggtgt
     6421 tccacggcca gggcacctac gcctggaaga acggcaaccg ctacgaaggg gcctggaacc
     6481 tgggccgcaa gcacggcccg ggacgcctga tgtgggcggt cggtggcggc tgggagggcg
     6541 agttccgcga cgatcagaag accgacgcgg ggcgcgacat caccgtggcg gcggcaccgc
     6601 gctgagcttc aaccggtgcc ttacacccac tggcggcggc gttccatcag cttccagatg
     6661 aggggggtga tgatcagctg catcgccaga tccagcttgc cgcccggcac cacgatggtg
     6721 ttggcgcggg acatgaagct gtcgtggatc atgcgcagca ggtaggggaa gtccacgccg
     6781 cgggggtcgc ggaagcggat gatcaccatg gattcgtcgg cggtgggaat gtcccgcgcg
     6841 atgaaggggt tggaggtgtc caccatgggc acccgctgga agttgatgtg ggtgtgggtg
     6901 aactggggca cgatgtaatg cacgtagtcg tgcatgcgcc gcaggatggt gtcctgcacc
     6961 gcttccatgg aatagccgcg ggtgcgcgtg tcccggtgga tcttctggat ccattccagg
     7021 ttggtgatgg gggcgacgcc gatgagcagg tcggcatgac ggcaggtgtt caccttgtcg
     7081 gtcaccaccg cgccgtgcag cccctcatag aacaggcagt cggtgtccgg tggcaggtct
     7141 tcccaggggg tgaagctgcc caccttgagg ccgcggcggg cggcttcctc gacgctgtgg
     7201 aggtaatggc ggcgctggcc ggagccggat tcgccatagg tgcggaacag gttttccagt
     7261 tcttcgaaga ggttggcttc cggtccgaag tgactgatgc cccgttcgcc ccggttttcc
     7321 gcgtcggtga tgagcgcgct gatctcttct cgggtgtagc ggtggtagct gtcaccctcg
     7381 atgatggcgg cgttgacctt ctcgcgccag aagatggcct caaaggtgtg cctgaccgta
     7441 gtggtgcctg cgccggacga gccggtcacc acgatgatgg ggtgcttcat cgacatggtc
     7501 gcatcctgaa agtgagtagt tttggagcag ggcacggggc catggcctgt tccaaaagta
     7561 ctccctgcaa gcgtcgtgcc gatgtcaggc gtgggcgtgc agccaggtcc gccattgttc
     7621 gaacagggcc gggctggcgg cggagatcac cgagcgcttg atggccggcc cggcggtcag
     7681 cggcgtgccg cccagagaac tgtagcagcc gccggcttct tcgaggatca ggcggccggc
     7741 cgcgtaatcc cacagcatct ggccgccgtg aatgtacacg tcgagccggc cggcggccac
     7801 gtagcaccat tccagggcgc tggagccgaa attgcgctgg gaataatacg gcgggcgtac
     7861 ggccagttcg tcacccaggt gatggctgat gcgcttgaag tccacgccgg cgacggcgtc
     7921 cttaaggcgc tcggtgggct ggcgcagggg cagggacacg tcgttgagcc aagcgccggc
     7981 gccgcgcccg gcgtaaaagg cttcgtccgt caccgggttg tacacgacgc ccagctgggt
     8041 cttgtggtcc accagatagg ccaccgcgac gccgaagaag ggaatgccgt tggcgaagtt
     8101 ggtggtgccg tcgatggggt cgatcaccca caggccggcc ttgcccgcgt gccacacgcg
     8161 gccctgttcc tcggcggtca tctcctcgcc cagtaccggc gcggcgatga ggtggggcag
     8221 gcgctcctcc agggccgcct gggcggccag atcggcttcg gtgagcacgc tgccgtcggc
     8281 cttgtgttca cgcacggcgc gcaggtagcg cggcagaatc acgtcacggg ccatcgcgcg
     8341 gacgagatcg gccagggcgc gggcatgttc gaggcgttgg gtgctggtca cggggcgctc
     8401 ggcctccact tgatggaaca gccgatgctt gcggtctggt cccgcgggcc ttcgccgctg
     8461 cgggccacct gcgccatggc ttcgaagagt tcgcggcgtg tatcggacgg ggcgggctgc
     8521 ctgcccgagg cgtcgaggcg gccccggtac tggagcttca gatcccggtt gaagccgaag
     8581 aagtccggcg tacacacggc accgtaggcc cgcgccacgt cctgcgaggc gtcgaggagg
     8641 tagggaaaag ggaagtcctg ttcgacggcg atgcgctgca tggcttccca ggagtcctcg
     8701 gggtagtcgg ccggatcgtt ggccatgagg gcaacgctgc cgacgcccag gcctgcaagc
     8761 tcacggcagt cgcgcaacag gcgcggcagg atggccttca cgtaggggca gtggttgcag
     8821 atgaacatca ccagcagccc ggccgggccg cggcaggaag cgaggctgtg ggtgcggcca
     8881 tccacgcccg gcagggcgaa atcgggggcc gaccagccga aatcgcagac gggaggggag
     8941 aggctgacca tgttcttcgt ttcaatcccg tgaaggggcg tggatttgcg gataatagca
     9001 tgatgaatcc gcgcttcttc tgccccgacg ggctgcagcc cgactccgag atgatcctgc
     9061 ccgcggccgt cgcccatcat gccgaacggg tgctgcggct ggcggtcggc gaaggcgtga
     9121 cgctcttcga cggccgtggc ggcgagtgcg atgccgtgct cgtcgcgctg ggacgtcagc
     9181 cgctggcgcg cctcgggccg cggcgtgcgg tggagcggga gtcgcccctg gcgatcaccc
     9241 tcgtgcaggc cctggcggcc ggcgacaaga tggactgggt ggtgcagaag gcggtggagc
     9301 tgggtgtcgc ggcgatccag ccggtggcgg cggagcgctc ggtgctcaag ctggccggcg
     9361 agcgggcggc caaacgcgtc gcccactggc agcagatcgt cgtgtcggcc tgtgagcagt
     9421 cgggacgcaa ccgggtgccg gaggtggcgg agatcctgcc cctggcgcgc tggctgggta
     9481 cgccgttcgc ggggcggcgt ctgattctgg cgccagggga cgcaggcgcc ctcgtgcgca
     9541 tggcgccgcc ggacggcccg ctggccgtgc tggtggggcc ggaagggggc tggtcgccgg
     9601 ccgaactggg gcttgcgatg cgcgctggct gcgagccgct ggccctgggg ccgcgggtct
     9661 tccgtagcga gacggcgggc ctggcggcgc tggcagcgct gcagaccctg tggggcgatt
     9721 ttccgggagg ctgagatgtt cgaatccgcg gagctgggac acaagctgtc caaggccgag
     9781 tacgccgaga tcgagccggt gctgcgtacc gagctcctcg aaacccagct cgatctgctc
     9841 gacaagaagg attttgccgc ggtggtgctg atcaacggca ttgacggcgc cgggcgtggc
     9901 gagacggtga atctgctcaa cgaatggatg gacccgcgcc acatcgaggt ccacgccttc
     9961 gatcggccca ccgacgaaga gcgcgagcgg cctttcctgt ggcgcttctg gcgcgctctg
    10021 ccgccccgcg gcaagatcgg gatcctgttc aacaactggt attccgagcc cctcggcgat
    10081 cgcgccttcg ggcgcatcaa gaaggcccgg ctcgaccagc gcctgtcgga gttcaaccgc
    10141 ttcgaggcca tgctggcggc cgagggcgtg gtgctggtca agttctggtt ccacctgtcg
    10201 cgggacggcc agaagaagcg cctcaaggcc ctggaggcca acccggcgac ccgctggcgg
    10261 gtgcgcgcct cggatcggct ggtctatgac cattacgacc gctaccgggc gaccgccgag
    10321 cacgtgctgc gacggaccag tacggcccat tccccgtggg tgatcgtgga cgggtccgat
    10381 ccccaatatc gggcggtgtt cgtcgggcgg accctgctgc accgtctgca ggcccacctg
    10441 gagcacgtgc gcaagggcta tcgcgcccgc ttggtcagcg cgcccttggt ggcggccgcc
    10501 gaccggcgca ccctgctcaa cgccctggcg ctggatcagc ccatggagcg caaggcctac
    10561 gaggcccagc gcgaggccct gcagggacgt ctgtccctgg ccctgcgccg gcgcggcttt
    10621 acccagcgca gtctggtgct ggtcttcgag ggcatggacg cggctggcaa ggggggggcg
    10681 atccgccgtg tgacgcaggc gctcgacacc cgtcagtacc acgtgatccc catcgccgcg
    10741 cccagcgacg aggaacgcgc ccagccctac ctgtggcgct tctggcggcg cctgccgcgg
    10801 cgcggcaggg tggcgatctt cgaccgttcc tggtacggcc gcgtgctggt ggagcgggtc
    10861 gagggcttct gcacggaggc cgactggatg cgtgcctacg ccgagatcaa cgacttcgag
    10921 gaccagctcg tcagtcacgg cgccctggtc gtgaagttct ggctgtcgat cagcgccgac
    10981 gagcagctgc gccgcttcaa ggcccgcgag gaggcggcct tcaagcgcta caagatcacc
    11041 ccggacgatt ggcgcaaccg ggagaagtgg ccgctctacg agccggcggt gtgcgacatg
    11101 atcgaccgca ccagcactga gaacgcgccg tggaccctgg tggaggccga caacaagtac
    11161 tttgcgcgcc tgaagatcct gcgcaccatc gtcgagaccg tggaacgcag cctgaaggcc
    11221 tgacggcgcg gctacaatga tggtcagccc gccgtgcatc cccaggacac ggtccgcctc
    11281 cctgttctgc cttctggaat gcccgtgaca gtgccccaat ccgttcccgc cgccgtcccc
    11341 gatcccggcc tctcgcaacg cttcgtcgcc tgggtgcgcg cagctgcgcc ctacatccac
    11401 gcgttccgcg gcaagacctt cgtactgggt ttcggtggcg aggtggcggc gggcagcctg
    11461 gcccagaccc tggcctacga ctgcaacctg ctggcggccc tcggcgtgcg tctggtgctg
    11521 gtccatggcg cgcgcccgca gatcgaggcg gagctggccc gtcgcggctt gcagccccgc
    11581 taccacaagg gcctgcgcgt caccgatgcg gacgccatcg aatgcgtgaa ggcggccatg
    11641 gcggtgaccc gcatcgaggt ggaggcgctg ctctcccagg gcctgcccaa cacgcccatg
    11701 gccggcagct tcatgcgcgt gaccggcggc aacttcgtca ccgccaagcc tgtgggggtg
    11761 gtggatgggg tcgattacgg tttcaccggt gccgtgcgca aggtgattgc cgaggagatc
    11821 gaggccgacc tggatcagca gaacgtggtg ctcatgtcac cgctgggcgt gtcgccggcg
    11881 ggggagatct tcaacatgag catggaggcg gtggccgagg ccgtcgccat cgccctcaag
    11941 gccgaaaaac tgatctatct gtgcgatgcg ccggggctgc tggacgaggg cggtgggctc
    12001 atcgaggcga tcaccgcgga cgacgcggag gccatgctga aggccggcaa gggcctcacc
    12061 gaggatctgg acctctacct gccctgcgcc atccgtgcgg tgcgcaaggg cgtcgggcgt
    12121 gcccatctga tcgaccggga caaggacggc ggcctgctgc tggagttctt cacgccccag
    12181 ggcgtcggca ccgtgctgac gcgcgatccc ctcttccgcc tgcgcgaggc gaccatcgag
    12241 gacgtggggg ccctgggcag catgatcgcg ccgatggagg ccgacggcac cctggtgcgt
    12301 cgcagccgcg aacgcctgga gcaggaaatc gagcgcttca cggtcgtgga atacgacggg
    12361 gtgctggtgg ggtgtgccgc actctatccc ttcatcgacg accgggcggc cgagatggcc
    12421 tgcgtggcgg tggtgcccga gcatcgccgc gccgggctgg gcgagatgct gctcaagcgc
    12481 atcgaggctc gcgcccgggc gctgggcgtc gagcgcctgt tcgtgctcac cacgcgcacc
    12541 tccgactggt tccgggagcg gggcttccgt gccatgccac cggaggaact gccgcgtcag
    12601 aagcgtgaaa tgtacgacgt ggaacgccgt tccaaggtgt tcatgaaggc actctgaggc
    12661 acggatcggt catgtctgcc atctgttccc ggatctgcgc cctccgctgg cggcaggtcg
    12721 gggccgccgt cgttccgtcc ttgtgggcgg ggggcgcgat ggccgcagag gcctggcccg
    12781 gtggctcgct gccggcgctc gcggtggtgc cggcgctggc tgccgcggct ctgtgggcgt
    12841 gcgtcctgtg gcggcgtatc cgcgcgctgg aagcgacccg cctggacccg cctgtgccgg
    12901 ttgagccggc gtcccgcacg ggactcccgg tggcgtcccc tgccgccgga ccgtgggagc
    12961 attactttgc gctggcggcg gactcgggcc atgtgctgga ggggctgatc gacacgcggg
    13021 ggcgtctcta ctgggtcagc ccggccttcg agcgctttgc cggccatgcg gtggaggatg
    13081 tgctggcggc gcccgacttc gtcgaactga tcatctatga gcgggaccgt cgctatgtgc
    13141 gcgagcagac cacccaggtg ctcagcaccg gccggccggc cgatttcgag acccgcgtgc
    13201 tgacgcggga gggcgccctg cgctgggtga cctgccactg gcacgcggtg cgggacgagg
    13261 cgggcgtggt gcagggcatc cgtgccaccg gtgacgacat ccaggcgcgc aaggaggcgg
    13321 agttcaagct ccttgagacc gttgcggccc tgcgtcgcgc ccaggctctg tccgagcact
    13381 atctgcgccg cagcaacgaa gaacgctcgc gcctgtcggc cctgctcgac gtggtggagg
    13441 cgggcatcct gttcatggat ccggaccacc gcgtggtgta ttgcaaccgg gcactgtgcg
    13501 agatctggaa agtggccgag ggcgagagca tcgtcggtgt gcgcgacgtg gtcctgcagc
    13561 agcatgcggt gaccctgctg accgatccgc cgggctatct gcgccacgtg gccggcgtgc
    13621 tcggctcccg tgaggccagt gcacccttcg agctgcattt cagcgacgga cggacggtca
    13681 atgaattctc ccgtgtggtg ttcggggacg acggggcgcg ggcgatcggt cgcatctggg
    13741 tgtatgagga cgtgacggag cgccgtcgcg tggccttgca actgctcaat ctcgccgaac
    13801 gcgaccccct gaccaacctc tacaatcgcc gccgttttca tgaagagctc gagcggatgc
    13861 ttggcgacgc ccgtcgccgc ggcgatgatg tggggctgct ggtgctggat ctggacggct
    13921 tcaagccggt caacgacgcc tttggccatc aggcgggcga ccaggtgctg accgggctcg
    13981 cccaggcggt tgccgccgtg gtgaggcgca acgagatgtt cttccgtctg ggtggagacg
    14041 agttcgcgat cctggtgccc tcggccgatg tgcaggggct ggccgggctg gcgcggcggg
    14101 tgggagcgtg catcgcagcc ctgcggttcg atttcggtgg cagcgcggtg ggtgtcaccg
    14161 ccagcatcgg cattgcctgg agcggactca gcggcaccga cgccgaggcg ctggtcgggg
    14221 cggcggaccg ggccatgtac cgggccaagg cgcagggcgg caatcgctcg gaggtggcag
    14281 cggcggcgga gggcaatgat cctgaagtgg gcgctgagcc gggcttgggc tagaatcgtc
    14341 cggcaagccg gcgcgcgtgt ggcggtgccg gattcctaac tgagaggtga tgcatggtca
    14401 ggatggtcaa ttgtgtgaag ctcggccgtg aggccgaagg gctggatatg ccgccattgc
    14461 ccggtgatct gggcaagcgc gtgtacgaga acgtttccaa ggaaggctgg cagcaatggg
    14521 tgcggcatca gaccatgctg atcaatgaga atcgtctcaa tctgatggac acgcgggccc
    14581 gcaagtacct tgcggagcag atggagcgct atttcttcgc cggcggggcg gaccagatcg
    14641 gtggttacgt gccgcccaag ccctgaatcc ggcggtttac ggcaacggac ggccctgcgg
    14701 ggccgtttct ttttggtggc gggcgtggtc ttcgaggctg tacagccagg caagcagttc
    14761 cgcgaccgcc acgtaaagtt caggcggaat gcgggcgtcc aggtccacct gcatcagcag
    14821 ggcgaccagt tccgggcttt catgcacata gacgccggct tcgcgggcgc gggcgatgat
    14881 ttctccggcg atcaggccgc gtcccttggc cacgacactt ggcgcaccat tgccggcgtt
    14941 gtacgccagg gcaacggctt cggcgcgggg gatttcgggc tgggcggggc cgttcatgcc
    15001 ggttcccgct gttcggtcga gacgccggtc agaggcaggt tggcggcggc aagggcttcg
    15061 gcaaggcgcg gctgtccggc acgcagggcc gcgaccgtgt cggcgctgtc acctgtcagg
    15121 cgcagtgcga ccccggcagg ggtgaggtgc aggcgggcct ccacgccacc cagggtgggc
    15181 aggctgaggc gcagggtggt gttccaggtg ggcgccgggt cgtcgtcggc acgggggcca
    15241 tcgcgggcgg ggtcctcaat ttcccattcc atggtctgac ctggccagat ctgtccctgc
    15301 cagacgaggt gctgggtggc gagggcatcg agttgctgat ggaccaccgg gagcagacgg
    15361 tcggggatct gggtggtggc gccgggctgg gcggcttcgg ggagggcggc ggtgcctgtc
    15421 tgcaaggccg tggtgggcgc agcggacggg gccgccggcc gcccctgggg ctcccgcgtc
    15481 aactgggcca tgtcgacctg gccggcgagc cagcgcgcct ggtgggattc atagaacagg
    15541 ccgctgtcgc caagcgctgc gcccagacgt tgggcgagca ggtcggtgtc accgggcccc
    15601 ggaggcgagg ccagcacggg ttggccgcca ttgaggctgg cggcctgggc gggtggctga
    15661 ccggtcagga gaaagctgat gaggcgcccg gcctggctca gggcggtgcc ggtctgggcg
    15721 ccgggcaggg cggtgctgcc tgcctgggtg gcgaacacgg cggccggcgt gacgtgactg
    15781 accacgagtt ccagagtgtc gccgggcgtg gcggtctggg gcagggcgag ggtgacgctg
    15841 cggccctcga cgagggcctt gaaggtgttg tcgggaccgc tctgctggag gcgggccacg
    15901 atgcgctgac ccggcaggaa atcgggcaga ttggccggca cctcacggac acgtgcgagc
    15961 ggcgccaccg tgggctcgcc ggagaagaag ctcgcctccg tgagcatccg caagcgggcg
    16021 gcgaggtcgc tggggatcat ggcgccgagg gagcgatgac ggacgggacc ctgcgccccg
    16081 ccctgtgaac cgcgtcagcg gtcgagcttg tcgtaggcgg cgcggacagc ccgttcgcgg
    16141 gattgcccgg tcagcatctg gcggacgttg tcgagccagg gctcggtgtg gacgcggatc
    16201 tccttgtcgt cgtcgaggat gcgccggatc aggtcgatgc gccggctacg ctcggactcg
    16261 gacatgcgac gccgggacgg ggagcccagg gcctcctcga cgccgatgcg gtcccgcagg
    16321 gacgcaacct ttttttcgag gcgggtgagc cggtcccagt cgttgtgccg ggcggcatcc
    16381 accatctcgt tggagtaggt gctcatttct tccaggagtg caagggaact catggggtgg
    16441 ggtctccggg gtcacgcgat cctgccggtc ggctctccgg agccggtctg gagatcgctg
    16501 ctgacgggtg gaattgtaaa catgaatcct gaagcggtgc ttacatgctg ccgtccgtgc
    16561 cggggccgat ctcggcccac gcgctcttga gctcggacag gagcttggtg acctcgtcca
    16621 gcgccggccg gctgttgtgc aggttggcgt agaggagacg atgggtcatg tagtcgtaga
    16681 gggcgcccag gcgggcggcc agttcgccgc cggcttcata gtccaggctg accttgaggc
    16741 cgttggtgat gatctcgatg gctttcgaaa cggctgcgcc cttgcccggt acattgccgg
    16801 attccatggc cgcagcggcg gcgccaacct gaagcagggc gccgtcgaaa agcatgagga
    16861 tgagctggtg ggggtctgct gtggaaactc cggtttccac gccgacgctc gcgtaggcgt
    16921 cggctctgcg ggcagcgagg ccaaaggaca tcacaatctc cgttcgggct ggcggggtgc
    16981 gcgcatgcgc ttatttgctc tgattggcca gcagggtgag ctgctgggtg agataggtgc
    17041 tggtggtggt catgctgctc atggccgtgt cgagcgccgt gaattgggat cgatagttgg
    17101 cttcgatccg cgtcaggcgt acgttcatcg aatcgatctg gttgccgatg ctcttgatgg
    17161 aggcgttcag cccattggtg cgcgatgcga tcacgccgtt ggtgcccagc aggttggtgg
    17221 ttgtggtctt cagcgcgctg ccgtaattgg cgatgacggt tgcggtcgcc acgtagtcct
    17281 tgctggccgc cgtcgcgaat ttggcggtgt cgaaggccag gctgccatcg cgctggaagc
    17341 tgacaccgat gtcgctcagc agtttgatgt tggcgttgcc cgacgagagg gtcggtgtgc
    17401 ccgtgagggc ggcgcggacc tggctctgga tgctgcgcag ggacgagtcg ccattgagga
    17461 tggcggcgga cttggtggtg gcatcgtagg cgccgaggct cttgatggag ctgttgaggc
    17521 tgttgtaggc gttgacgaag gcggtgacct tgccctgcag gtcgctgttg ttgcgtgcga
    17581 ccgtcagggt ggtgctgcct gtcttggtga ggttgagggt gaccccgtcg atggcattgg
    17641 tgaccgtgtt gccgctggtg gcgatcgaca cgccctggat cttcacgacc gcgtcggtgg
    17701 ccgcctgggt ctgggtgaag gcgctggccg cggcaatcgg cgagccgggg gccgacgcgt
    17761 cgatgaagct ggcgtcaccg gagatcgtga ctgcgttggc ggtgccgccg ttggtggcgg
    17821 agagcagcag gttctgcttg ccgtccgtgc ccgtgacgat ggttgcgccg acaccggcgt
    17881 tggcgctgtt gatcgcgtcg cggacgccgg ccagcgtgtt ggcgctgctg ttcagggtga
    17941 cgttggtggc cgtcgagccg acggtgaagg tcagcgtttt ggtgctggca ctgccgaaat
    18001 cgatgaccgg gtcggtcgat gcgtaggttt tgcccagggc cagctgctga ttggtggcga
    18061 gtgaggtgac gttgacggag taggtgccgg ccacggcgga agcggtggcg gtggcagatc
    18121 ctatcgtggt gtcggcaagt gttgccgtat aggcggcaaa tgctgccgtc ggggactgcc
    18181 cgattgccgg ggtgagcgct gctgcggcgc tctgcagcgt tgccagattg ctcttgaggg
    18241 tgccgatggc ggagatcttc gactggtctt tgttctgcag ggtctggagc tgcgtgagcg
    18301 gctgcttttc gaccgacatg agcgacgtga cgatcgagtt gacgtcgagt ccggagccga
    18361 gccctgcaga ggagatgcta gccatgatgg ccccctgtaa tgcctgtgaa cacgctgttt
    18421 ctcttacgtc ccgcgaggct gaaacttgag cggaaacgca ccagtcgaga agtagcaggc
    18481 tccatgccag atgcacgaac ccgcgaaaaa atcccaccgc gagggtggga tcggcgatgc
    18541 ccggcgcgga ggtggatgcc tcaggcctgt tgcttgacca gcaggccctg cagtttgtcc
    18601 agggccttgg cgatggccat gacttcctcg gacgggatct ggcggatcac cttcttcgac
    18661 tcggtgtcga ccaccttgac gacggtcatg ccgctgtctt tgtcgatgga gaactggagg
    18721 ctctgggcgg tctgcttgat gacgtcctgg atctgtttga cggcctgatt gagctgctcc
    18781 ggggtcggcg gcgcggcggg gttctgggtg gcgtccctga cagcctggac gtcgccgggg
    18841 gcggtggggg cgtccgcctt ccggatcgcc ccctgggcgc cgggtgtcga ctggacggga
    18901 ggcgtgtaag gctgggtgct ggccccgccg accggttgga tgctcatgat cttttccccc
    18961 gagatgtgca gacggcgccg cgccggttcc ggcggcggcg cccgctgcgg cgaatttcaa
    19021 ttccgcgctt acttgagcag cgacagcacg ttgttgggca tgctgttcgc ctgggccacc
    19081 atcgccgtac cggcctgttg caggatctgg gcgcgggtca tgttggcggt ttcctgggcg
    19141 aagtcggcgt ccatgatgcg gccgcgggac gacttggcgt tttccgacgc aacggacagg
    19201 gtctgcacga cggaactgaa gcggttctgc acggcacccc aggtggcgcg ggaggtgttc
    19261 agcgagtcga tggcggcgtc gatggcggag atcgcagcgg tggcgcccga agaggacgaa
    19321 cccgagatgc tgctaccgct cagggcgccg gccgtggtgg tgccgaaggt cgccgacgcc
    19381 agggagcctg ccgtggtcac cgaaatctgg tccgtgctgg cggtgcccgc gccgacctgg
    19441 aaggtgaagc tggcggtgaa ggtcaggatg ttctgaccgt tgaactgggt gccggagaca
    19501 acccgcgcga tttcctggtt cagctgctgg aattcggcat ccaggttggt ccggtcgccg
    19561 ctggtgtagg taccgttggc ggactggacc gcgagttcgc gcatccgttg caggttgtcg
    19621 gtgatcgcct gcatggcgct gtcagccgtc tggcccatgg agatgccgtc gttggcattg
    19681 cggatcgcca cgtcgaagcc cttggcctgc gcgttcatgc ggtcggcaat ggcgagaccg
    19741 gcggcgtcgt ccttggacgt attcacgcgc aggccggagg acaggcgctg aaccgatgtc
    19801 gccagactgg attgggacat ggaaagattc cgctgcgcat tcagcgacat ggaattcgtg
    19861 ttgattgtgg cagccatttt ggttctcctt aattccgttc aattcaagat aagcgtttag
    19921 gcttaacttt ggttcgcctt gcccggtgtg tgtaaggcaa ccgccgttga gttgattaac
    19981 ggggccacaa ggaaatactt tagaaatttc ttgactttta caatttgatg tgttttgcgg
    20041 tgttctcggc ggattaccgc ttggcttgtg atagcggcgc attccgagga agctttagcg
    20101 tgcaaagcac gcgccagcgt gggttttcca cgggttgatc acaggctttc aggacacatg
    20161 cacgtgaggc ttcagggtcc gtaggcggca aaccagcgtg ccgtgtggtt ctcgaggaca
    20221 tagccctcgc gaacccagcg ctgcagggcg aggcccgcgg ccttgagggc ggcgggttcc
    20281 gcaatctgtt cccggatggt ccggatccag aggtccgggt cgttgggaag gcgggtgacc
    20341 ggtgcattct ggtacgggta gatgtcggtg cagatcaccg gccagcccat tgcgccgtat
    20401 tcaagcaggc gcaggttgct cttggcctcg ttgaagggat ggacgtcgag gggggcgatg
    20461 gccagatcca gatcgaggcg gctcagggcg cgggggtagg cccgaaaatc cagctcgaac
    20521 ccatgggctt cgtggacgaa gccgcgaatg ggttccgggc acatgcccat gaagatccag
    20581 tcgacctcgt cggccagggc ttcgaccacc ttgtagatga gctccaggtc tccctggtgc
    20641 tgctgggcgc cggcccagcc cacgcggggc ttcgtgcgag gccggccggg gggcgccacg
    20701 tcgccccaca gggaccactc gaggctgttg ggcatgacga ggatgtcgtc gatcatgtcg
    20761 gcgcacagat cggccagcgg ctgggtgctg accaccaggc ggtccatgaa ggacagcacc
    20821 cggcgcaggc gctggcgccc atccttgtag ccggacttgt agagcggatg cttgcgcggc
    20881 aggttggtga ccaggtcatc catcgtgagg attcggcgga ccttgggcag aaagcgcgcg
    20941 tagtgttcga gggcgtcgct ctgggtgtct tcgaggggct ggtggaggat caggacgtct
    21001 ggatcggctc gggccacctc gacgggcgac aggatgcgca tcgtgaacgg cttgggcggc
    21061 tcgcagatca tggtctgggc gaggccggcc tgaccgatga cacggagggg cgcgcgcagc
    21121 cggaattccc cggcaccgcc gggcaccggg caggcgagga tgcgcgggcg gtcatggaag
    21181 tggggatccc agtccatggg ggctacgtgt tccggcttga aggggaagga caggctgagg
    21241 tggcggttat aggccggatc gctgaccagg cgtggcatcc atcgcgcgag cagggtctgg
    21301 tttgcctcct gggcggcgat caggtcggtg agcttgcctt cgaggcggcg ctggcgcgcg
    21361 acgacggagg cgcctgcgta gtgaaccagc gcggcgtagg gcgtccagac aatcttgtga
    21421 ccctgggctg tggcccgcag gcacaggtcg agggcgccgt agaggagcgg aaaccgttcc
    21481 gcatcgaagc cgttcagtga cgtgagcacg gcgcgtttca ccagcagcgc ggagccgctt
    21541 gccgcgctga cgttctggtc gcacagggcg cggcccatgt agccggggtc ggtcagtccg
    21601 agctggttgt cccaggggtt gccggtggtg ccgtgcatgc cgaggatcca cacgccctgc
    21661 tgaaccttgg cggtttccgg gtaggtcagg cggggggcca cgatgccgac ttccgggcgc
    21721 tgggcaatgg ccacgaggcg gtcgagccag tcgtcctgga cgatgtggag gtcgttgtcg
    21781 aggaacagca ggaagtcgcc ttcggccacc gccgcgccgg cattggcgca ggccgaccag
    21841 ctgaattcgc ccggctggcg caggatccgg gtgcgcaccc gggcgtggcg accggcgagg
    21901 gcggcgagat agtccagcgt gtccggatcg acgctgtcgt tgtcgacgat gatcagttca
    21961 taggggcggt agcgcgtgcg ctcttccagg ctctcgatga aggggcgcag gtattcgacc
    22021 ttgtcccggg tggggatgat gatgctgacg aggggggatt ccgcccagcg gcagatgacg
    22081 cgccgcgtgc cgtagccggc gccgtcgagg acttcggcat cgaggccgcg gcggagcaga
    22141 tgggcctgga cggcggcctt ttccgcttcg ggctgcatgg cgcgcacgga ggcccgcggc
    22201 agatgggcga gcaactggtc caggtgaccg atggcggctt cgccggcgtg atcgaggatg
    22261 cgcagggtga gatcgtagtt ttcggcgccg gccagggtgc cgaaacggcc ggcggccagg
    22321 aaggcctgct tctcgacgac gatcagactg ccgacatagg cctgggcacg cagcagatcc
    22381 aggttgaagt cgggcttgaa gagcgggtgc gtgtagcggc cgtccgcctc gagggtgtct
    22441 tcgtcgcagt agatgagacg ccagtcgggg cggacattga tgtaatcggc gatgacctgc
    22501 agcgcgtggg gttcaagggt gaggccgggc tcaatgcggg ccagccaggt gccgggcgag
    22561 gcggccgcca tctcgtcgat gacgtagtcg atgtgcgcgg catcgcgcag ggcgagccat
    22621 tgcagattgg agatgtcgcc gacgccctca ggcatgtcga gggcggtggc caccgtgagg
    22681 cgccattcgg gatagagctg ggccgcgagg ctgtccaggg tgtcggcgag gaggtgctcc
    22741 tcgcccgcat gcagggccag caggaggtgg atgcccgggc gctgctgcca ggtgagcatc
    22801 atgcgctcgg ccaggatctg ggcgtcgatt tcctggaggg tctgccgggc acgccaggtt
    22861 tcgtattcgg tgtcgtcctg ggtgggacgt cggctgctgc gggtcgtggg gtcgggcttg
    22921 ggggccgcgt cggcgcgcgc ctgggtcagg gtgatcagac cgtcgagcgc aggccagaag
    22981 gcggcctcca gggattcgta gagcgtcgcg atgtggccca ccgtgcgggc cgccgtggcc
    23041 agatcctcgg gtgactgggt gaaggcgacg ccttgatcga atccctgacc gcggccaaag
    23101 aacttttcct tgctgaagaa ggggctcggg agcatgacca ccgggcagcc gcacaggcgt
    23161 gcttcggtgg agatcgcggt gttttcgaag cagtagaccc gctcgctgcg ccgcaggagc
    23221 tcggccagct cttcgtgggt ggcgggaaag tcctgggtga tgacggtgca cgtgtccgcc
    23281 aggtcagggt gttcctgctg gcccgcgctg tagcggccga agtagatgca gcttccgctg
    23341 cggtgggcgt cgtggggatt gtcgcggttg ttgaacaggg acgtgttgat ggacggcagg
    23401 cgcagcacgt cggtggcgcc gggcacgatg tcgagcagct ccggttcgta gacgtaatag
    23461 atgtcgtccg gcccgtacgc cggctgttca ccgagcaggc ccgggcggtt gaggatgtag
    23521 cgcaccacca ccgcgcagtc cagcgggttg gcggccacca cctcgggata gatggcgatg
    23581 ggcgtcaggc cgcgggcccg atgggccgcg cgcacctcat cggtgagccg tggggtgtcg
    23641 agggtgggat gcgtcacccc ggtctggatc caggcatctt cgccgctctg ccgcagggcg
    23701 tggcacagct ggtgcatgac acgaatgccg gcagacgtcc gccggtagtc cggcgcgacg
    23761 atgtaatagg ggtgacgggg aggcggatag agtgagcgca tggcgtcctg ccgggctggg
    23821 agcactgggg cccgcatgac acgggcggtc aagggcgaat caggcgggcg cgaggacggt
    23881 cacgccgctc caccagtatt cggccgtctg gtccaggtgg ggacggcggt tgaaggtgag
    23941 gttgtaaccg taggcctcga agtggtagcc gatgccgggc ttttcgccct gcaggccggc
    24001 cagccacagc agcggcgggt agccgtcggt gcgcaggagc aggtccggtg cggcaatgaa
    24061 ggcggcggcc gtgtcgaagc cgcccgccag gagcaggtgt tcgggcaggc cggccatgcg
    24121 ctcgacggca gccggcaggg aatattcggt gaggcagggg aagtacaggc cgaccacttc
    24181 gccgtgggcg agggcgtcca ccaggatctc gtgacgggag ccgggctgca ccgagagctt
    24241 gccgcccagg ccctggtggg cgtggtcggt gaagcgggcg ttggggaagg cctcctcgaa
    24301 ggagcgggcg acggccggca gatagccttc ttcgagcagg gtgccgatct ccgtgaccgg
    24361 cgtgcgcggc aggatgaagg gaaccgcggg accgtccagc aggccggcgg tggcggggtc
    24421 ggcggccagg cggacgcgca gggcggcgat gcgctggatg aaggtgtcgg tgctcatgtc
    24481 cttgccggcg cccaggtggc gggcgaggcg ggcatggatg gcggccgggt cgagggccgg
    24541 cgtgccgagg cggaaatagc gccgcgagcg ggcgttggcc ggggcgctga cgccgagggg
    24601 caggcagcgg ccgaagtcgt cgaaggcggc gggggcgagg gtggcggtgt tcattgttcg
    24661 tcctgcagcg agtcgtggtc ggaaaatccc tggccccagg gcaggggcag ggtcacggcg
    24721 cctttggtgc gttcggtgtg gatgcggatg aactgcgggt cttcgccgat caggcagtcc
    24781 ggacggtgcg ggcccagcca ggggttgcgc atgccgcggt cgagaatctc cttgagcggc
    24841 gtctggcgca ggttgccgag ggagaagtcg atgtaggggc agggggtgac ttcggcggtg
    24901 gaggtgaccg tgatgatgcc cttgacggtg atgcagccct tgaacgagcc gtaagagggc
    24961 gtcatgtggg tgaacaccgg atgggtcttt tcgagctcgc ggacgatgtc ggcgtcttcc
    25021 ttgtcgatga cgaattccgg gtggttggtg cacgagccgg tgggcttggc gtaggagacg
    25081 tacaggccga tgccgcgggc tttggcgaag tcgcacatct ggcggaattc gtcggtgcgg
    25141 gcacggccgc gcaccagcac ggtggacagg atgaggttga ggccggcctc ctgggcggcg
    25201 tctatggcgc gcatgacccg cttgtaggag ccgggcttgt tgcggaaggc gtcgtgctcg
    25261 gcttcgacca tgctgtcgag ggagagctgg accttctcca cgccgacgga cttgatgtgc
    25321 ctggcccggg ccaggtcgag gaaccagccg ttggtgtcgg tgatgacgta gtggcgttcc
    25381 gggtcgatgg cctcgacgac ggcgtcgaag tccttcatca ccaggggttc gccgccggtg
    25441 atgacgaagc gggcaaggcc cagcgcgtcg gcctccttcg acaggcgacg gatgtcgtcg
    25501 aggtcgatct tctggcgctc gtccttgatc ttgaaaacct tctccaggtg ccggtccatg
    25561 tagtactcgg ccgagcagtg ggtgcactgg aagttgcaca ggtagctctt ctcgagccgg
    25621 atgatggggc tgatctcgcc gcgttcctcc atctcggcaa tgcgggcgag cttggcggcc
    25681 acgtagggtt tgcgggtggc gaggtcgttg cgtttggccg cctcggcggc ggtcaggccg
    25741 gaatcgggaa tcagcgcagt ggtcatggcg tccgtctctt tctcgggtcc ggtccgtcgg
    25801 tgggccgggg ggcgacaggc tgtcggggag ctcgggcctt ttcgaggcac ttccggggga
    25861 tggccggttc ctgaaaaagc gcgatggtgt cggcacttac ggcggcttca gggctttctg
    25921 aagggcacat tgccatttca ggtgtcgcac ccattgtgcg ggtgctggcg gtggccggat
    25981 ggccggaata ggggggagag gcgggcctaa ttccgtgctt gggtcggggc ggcgcccgcg
    26041 cgggccacgt cggcggtgag gcggatggcc tcgtcgaggg gcgtccatac gtcgaggccc
    26101 agctcgccac gggcacgctc gatggagggc acgtagacgt tgcgggtggc tacgtcggtc
    26161 gtcgtgccga ggacgcgcac cggcaccgca ggcgccacca cacgggcgac ccgctgtgcc
    26221 agatcggcga tggtgacggc ctggtcggag cccacgttgt aggcgcgccc gggtgcgccg
    26281 cgcaccagca gggtgagcag ccacaccacc aggtcgccgg cgtagagata ggcgcgtacc
    26341 ggtgtgccgt cgccgccgac ggtgatcgct tcgccgcgca gggcgtcgcg gatgaagttg
    26401 ccgatggcgt agtgcaggtc gaggggcagg tgtgggccga cgaagctgaa gcagcgggcg
    26461 gtgctgatgg acaggccgtg gcgttcggcg taggcggcgc tgaggaattc ggcggcgcgc
    26521 ttggcgtggc cgatggccac gtcgaggttg tcgggggagg gggcgacggg caggtcttcc
    26581 gcgtacgccg cgcggtcggc cggcaacggg ccatagaatg cgccggagcc gagcatgagc
    26641 aggcgcggga tgcgccgttc ggtggcgaaa tccagcagcc ggcgggcgcc ttgcagggtg
    26701 gtgtcgaact tggtgagggc gtcctcgcgg cgatggaagg tggccgcggc ggaggtggcg
    26761 gcgccatgga tcgcgtgggt gaagtcgccg tccgggaagg caaagtcgcg cacatcgccg
    26821 ggatggaagc ggaccgccgg gctggcagcg acttccggcg cgtcggccag gaagcgcgcg
    26881 ggatcgcggc tgaggacggt cgccgtgagg ccgagcccat gtacggcgtc cgcgcggatg
    26941 agggcgggca gcagccagcg tccgaagtag ccggtgccac cggtgatgaa caggcgcgcg
    27001 ccgcgcaggg ccgtccagcc gtcgccaaag cggacgacgg cgcggtcgat ctcggcggtg
    27061 aagcgctgcg aggcggtcat ggcgggtcag cggctggcgt agagcacggt ctcggcgggg
    27121 aagccggtgt agttgcgcag gtagaagcgg taatccggca ccagcgcgtg tatctgcagg
    27181 gcgatctccc agaggtgggc cggatggtga tagacgcaga tggcgaggtc gggtcggttg
    27241 gcccggatga gctgtcgggc tccgcgcagg gcgtggggtt cggccccctc gatgtccatg
    27301 ttgatgaagg tcgggcggaa gtccggcagc acgtggtcga gggcgacgca ttggacgacg
    27361 gtgtcgccgt cgtcgctgag ggtgctgttg atgcgattgc ccccggcgaa acgcagctgc
    27421 acgtcgtcgt cgaacacgcc gcaggggaag gccatcacgg tgccggcgag ctggccggca
    27481 tgctcggtga gggtggcgcg caggcgggtg aagttgggca ggtcgggctc aaagcaggca
    27541 agggcctcga tgcggccgtg acgggcgtga agctggcgca cggtgtcgcc gtcgtaggcg
    27601 ccgcagttga gggtgcgggc gacgccggcg gcgaggggta cgtcgtgggg gaagtactgg
    27661 tgctcgaggg ggtcgcgggg aatgggtacg ggcatctgcg cgacgtgggt ctgcagcacg
    27721 gcttcgaaga cgctgcggct ttttgcgtcg gcaagcaggg cgtaggcgcg ctcgatgacg
    27781 tcggcgcgtt cggcgaacca ggtttggccg agctgttcga agccggccgg ggcgtgggag
    27841 aggtggtatt cgtagaggtc cgaggccagc acgatgcgcg tgaagccgag gccgcgcagg
    27901 gtggcgaaga tgtccgcgtg gaaccgcgtc ttgccgacgg tgacgacggc cactgcgcgg
    27961 tcgagcaggg ccggatcggg ccggtagtcg gccggggcca tcgccgggat gtcgccgagg
    28021 gtggtggggg cggtgaattt ggcgtcaagg cagacctcag gccggatgcc gaatttatcc
    28081 agcacgaaca ccgagaaggt gatgagcccg tcgccgcagc catagacgac gaccggccgg
    28141 ccggcgagca cgtcggcgac gcggcagggc gccgcagggg tggcgggcgg tgtgtggagc
    28201 aggcggcgca gcggggagtc gggggcattc atggcgcggg tctagtggcg gcagaagccg
    28261 cggaaggcgg cgggaatcca ggcgacggcg atgtggcgct cgcggaagaa ggctttgacg
    28321 gtgtcgtcgt tgcggctgcc gtcgggcacg gcgccacagc gggcgccagc gtcggcgacg
    28381 atctgcgggg cgtcggtgaa ggggaagaag gcgtcggcgg cgaaggcggc gccggtggcg
    28441 tcatggccct gttcggcgca gcgggtgacg gcgctgcggg cggcgtcgac ggtagacggt
    28501 ccgccaccgg cggcgaggag catgccgccc ctggcaagcg ccacctcgtt gccgccgtga
    28561 ttgctgctgt aggcggcgga ccaggcgatc aggaggtcgg tgacggcggc ttcgtcgaag
    28621 cgggcgttgc cggcgcaggc ggccagttcg agcacgtagt cggccaccgg ctggccgagg
    28681 gcgccgccgc gcaccgggcg gagcatgcgg cggccgtggg gcaggcgcgc tgcggccagc
    28741 gcggggttgg ccaggagctt gcggaattcg cgcctgccca gcagttcggc ggcctcggcg
    28801 gtgaaggcgg gcgccatcac cacgtcgagc atccagtggc ggctgccgag gcggacttcg
    28861 cgttcgtcgc tggcgaagag tgcggcggcg agctcgccgt cgatggggaa attgacgatc
    28921 acctcgccac cccagatggc gcgggggttg ccggccaggg cgcggtcgat ggcttcccgc
    28981 ggcgcgtggc ggctgacgcc gatgccgcag gcgttaccgt gcttggcggc gatgcacagc
    29041 cagggaacgg tgcccgtgtt cttctcaaag ccttcggcca gcaggcacag atggtggagg
    29101 atggcgtcgg cgtcggcggc gttggtgaag cacggcggct ggctgccgag gaggcggaag
    29161 cggctcaggg cgagcgggtc cggatcgccg ctgcggtagg ctacggcggg gcgctggtag
    29221 gggttttcgc cttcggcgag gtcgagccag gcttcgccga agctggtggc gaagggggcg
    29281 tcgggcgaga tcaggccggc gaagtggcgg gcgacgctga agcaggcggc gtcggcgagc
    29341 tggcgccgca gggcgggcag atccccgccg gtttcgaggc aggcgagcac ccggtgcagg
    29401 tcggcccggc tggaggcggc gaggcgctgg cccttgacgg cgagcatcag gatcgtgcgt
    29461 ccgcccacgt cattgccgac gtcggccggg taggggttga tgtaggcgag ctcgatggcg
    29521 ggttcggcca ggtcggcggg gcgggcggtg agggcgcgtt cgagcttcgg atgcagggtc
    29581 ggcggaaagc cgaagtcctc ggtgagcgcc gtgtagtcgg ccaggtcgat caccggcagg
    29641 ccggcggcgg cgatcaggct ggaggactcg cgggaggcga gcagggtgaa gccggcgccg
    29701 atcagccgac gggcgtcatc caccaggccg gtggtgtcgt gcagggagat cagcgccagg
    29761 cgggccatgc tcagtccttc ctgacgcggc tgttctcgtc gatgcggtag tagatgttct
    29821 ccatccagtc ggggcagcgc acccggtcga agtcgatgca cgaggggatg tcgatcttca
    29881 ggaaggggaa caggttgttg aagccggcgg ggcacatgta ctgggtgcgc atgctgcggg
    29941 tggtcgggaa gacgatgcgg tcacccacct tgtgctggta ggggcagtcg ccatgcaccg
    30001 cgacgacctc ggcgtggatc acgtcgcgcc agtcggcatc gacgccggcc tcgaacttgc
    30061 cgtcgttggg gcgcaccttg accagcacgt ccacgccctt ggccgccggg cagcatacct
    30121 ggcagtcgcc ctgctcgttg tacgcgtacc gggcgccgaa cagtgccccg aggaaatacg
    30181 ggtacagcgt gtggtacatg atcgggcaga cgttgctcgg caggatgttg tgggggccgt
    30241 agtccttgcc gtgttccttg tgttcccggt gccacacgca gtcggtctcg ccgggcaggt
    30301 gcttgatgca cagatcgcag ctcatatcag gaagctccgc cacttgttgg gttgctcgta
    30361 cttcgagagg atgtcgttga gcttggagat gccgcacacg gcgcattcgt caccgcccgg
    30421 cggggtgccc atgatctggc gctcgacctc ctggcggcgt tcgccgcgcc agatctcctc
    30481 gaagctctgc gtgtgcaggc tgcccatgct gaactcggat ttgccccagg cgcggtagca
    30541 cgaatagaca ttgccatcgg cggccaccag cgggaagaat ttgatgccct ggcagtggtc
    30601 gtccttccag cagtccttgt acatgcccac gtggtagctg gtgtcatcca ccggaaaacg
    30661 caccttgacc gtgaagtcct cggtgttgag cttcagcacc tcggccttca tggcgtcgat
    30721 ctcggcggcg gtgaagtgtt cggaggtgtt gcgcgactgg ttcgacgggg tgaattcggt
    30781 gtaggtggct tcctgcacca cgatgtagtc gaggcccagg tcgcggtaga agcggatgat
    30841 ctggggcagg cggtcgaagt tgttgagttc gacgtacacc gtgcccatca gcgccacgtc
    30901 gagcccttcg cggctgcgcc ggttggcggc ggcgccgagg ttggcgatga gctggtccca
    30961 ctgcttgggc gagcagccgt gcatcagggc gtagcggtca cggtcgttct ccaggacgct
    31021 gcacttcatg aaggtgaggt gctgcaggat cctggactgg atcttttcgg tgaagaccac
    31081 accggtggtg gtgagcgagt agcgcaggcc cttcccggcg ccggccacga tggcgtcggg
    31141 caggccgcca tgcatcagcg gttcgcccgt gccctgcagg gagacggcct gcacgcccat
    31201 gccggcgagg tcgcagaaca gccgggtcag gatcgcgtcg gggatcttgt cgcccttggt
    31261 ctcgatcttg tcggaatagc agaacttgca cttctggttg cagtagccca ccgggctgac
    31321 ctcgacgatc accggggcga agtactgctt gtgcttccac gcgtgcagcg cctcgatctg
    31381 gaagaccgtg cgcggctctg cgtaaccgtc catgtggatc ttgtagctca tggtgactcc
    31441 tctctcagcg cgcggtttcg aggatcgtcc gcacgtcgtt ggggccgagc cgcacgaagt
    31501 tgccgatcgt gccgctcggg ttcttgcggg cgcagacatc caccaccgcg tccagggtgg
    31561 ccggggccag gtcgacgccc agtccggaca ggcgcaccgg catgccgatg cccgcgatgt
    31621 aatgctcgaa ggcggcgatc gcctcggccg ccgtgggtga ggcgaggccg aagatgcgct
    31681 ggccgaactg cacgaagcgg gcggggctgg tttcggccgc gcgcttcatc cagcccagca
    31741 tgacgatcac cgtgagggcg ccgtggggct ggtccacgtg gccgcccagg gcgttggcga
    31801 tcacgtggga tgaccagtcc tgcttgcgcc ccacgccggc cacgtcgtca tgggcgagct
    31861 tgcaggccca catgatctcg gcgcgcacgc cgtagtcgtc gaggcggccg tccacggcgc
    31921 gaccgtaatg gatgagggac tggatcaggc cctcgcacag acggtcgctc acctccacgt
    31981 agtcggtctg ggtgaagtag cgctcgcaga cgtgggtgat ggcgtcgagg atgccgcagc
    32041 ggctctggaa caggcccacg ccgcgggtgt tctcggggtt gagcacggcc agcgccggca
    32101 ccaggcactc ggcggcgaaa tcgagcttga ggctgcgcgc ggggtcggag atgactgccg
    32161 cggggctgga ttcgctgccg gcgccgggca cggtgaggat gactcccagc ggcagcgcgg
    32221 cggcggggcg cacgcggcct tcgatgaggt cgagcacgtc gccgtcaaag gccgcgccgg
    32281 cggcgatggc cttggccgag tcgatcacgc tgccgccgcc caccgcgagg agggtgtcga
    32341 tgccccgtgc gcggcacagg gcaacgcctt cgcgcacccg tggcagggtc gggttgggct
    32401 gtacgccgcc cagttcggtg acggcgatct gccgggcggt gaggccggcc atcaccctgt
    32461 ccagcaggcc gctgcgcgtg acgctgccct ggccgtagtg gagcagcacg tgtcgggcgc
    32521 cgaggcgggc gagttcggtg agtccggcgt cttccatgcc ggtgccgaaa tagatcgccg
    32581 tgcggttctt gtagaggctg ttgatcatgc ggggcgtccc gagaggaggt gccagtggcg
    32641 ctggggctgg cggccgctgc ggcgggtggg gcgcggcgcg ccgcggggtc caagccaggg
    32701 gcggtagagg tcgtgggcgt cgatcagcgg gtcggggcat gcggcgtgcc atgcccaggc
    32761 gccgtccacg ggggcggacg gatcgaagcg caggcgctcg tccggcggcg tgtcgagggt
    32821 gcaggcgtag agcatggaga tgaaatgccc gcgctcctcg cgcacgtcgt tcacgatctc
    32881 gtggatgcac aggggctcgg gctcgaagcg cacccgggcg cccagctcgc gggcggcgac
    32941 ggctgcgatg cgggtggcgg cgtcttccct gaagcggatg atgccgccgg gcacgtgcca
    33001 gccagggccg tagaaggcgt cgtgacgcca gccgaggagc atccgcccgg cgtcgtcgcg
    33061 gatcagcaga tccacgttga ccagcggtgt gaggctgctg acgaagagga acaggtcttc
    33121 cggcaggccg ttacggggcg ccgggcccag cgcggcgcgc agggtggcga tggcctcgcg
    33181 gagggcatcg gggctgggcg tggtcatggg gcaatgaccg tgtcgaggcg gttgtcgcgc
    33241 aacacggcga cggtgaagcg gctgccgtgg cgttcgcgca ggatgcgtgc gacctcgtcg
    33301 gagtagctcg cggcgagcac gatcaccgcg tccacctcgc cgtcggcgag gcgctcgggc
    33361 gcgacgatgg gccggtgggt ggccggggtg tagcggccct gcttgaaggg tgcggaatcc
    33421 accacgtagg cgacgcgcgg ggcgagcgcc atcaggctga tgagggccag ggcctggtga
    33481 ccggcgcccc agatcgccac gcggcccggc gcaaagcggg cgaggaaggc gtcgaaggcg
    33541 gccttgaggg cgtcctggca ggccaggagc gggctcaggt cgaggggacg gcgcttgcgc
    33601 acggtggcgg agagcacgta gtcgtgccac gccgggcggc agtccagcac gtcaaagccg
    33661 ttgcgttcca gcagggcggt gagggtctgc cgggtgaagt agaacaggtg gtccggaatg
    33721 aactcggcga acagccctgc gcgcaggatc atgtcgaagt tggggacctc cacgaggccg
    33781 ctggcgccgt ccgtcaggtt ggcggcgatg gcctgcagcg tgagcggggc ggcggggatg
    33841 tgttcgagga agttgaggat cgcgaagccg tcgaaggggc cgctggcgag gcggatcgag
    33901 ggatcttcga gaaagccccg gtgcgcgtgc aggccggcgg cgtggcaggc ggcgacggcg
    33961 gccggggcgt actccagacc gtgggcgtcg gcgccggctt cagccagcag ggccaggtat
    34021 tcgccgcgtc cgcagcccac ctccagcacc ttgcggccgg tcaggtcgtg ctcggcgatc
    34081 cagcgcccga actgttcccg gcggaaggcg cgcatctcgg cggacaggcc ggcggcgcgg
    34141 atcacctcgc gccagtaggg caccggcggg ttggcgagct gcacggtggc gcagccggcg
    34201 cactggtgca gggcaagggt gacaccctgg tcgtcggcga gctgctccgc ggtcggaaga
    34261 ccctgggcgg cggcgggcat ggcgtgctgg accagcagcg gttccgggaa gaggggatgg
    34321 ccgcacaggc ggcaggtggt ggacatgggc tcaggccggg cggggtgcgt cgagggaggc
    34381 gggcagcagg ggcacgatca tctccgcctt caggacgtcc aggggcagca gggggctcat
    34441 gtcctccagg ggcatggaga cgatggagcc gtcggcgcgg gggatcgccg cgcacttggg
    34501 ggcgaggctt tcgtcgggca ccaggtgcac gtcgatcagg caggggcgcg gcagggcctg
    34561 ggcgcggccg agggcgtcgg cgagaccggc gcagtcgtcg atgcgcagat agggcaggtc
    34621 gtagacggcg gcgagctttt ccagatccgg catgagcagg ccgccttccg gcccggtgcc
    34681 caggtagcgg ccctggaagt agttgcgctg ggtgttgcgg atggaggcgt agccgccgtt
    34741 gttgaggatg aacaggcaga cgggcaggca ctgcccggcg agggtggcga gctcctgcac
    34801 gttgagctgc aggctgccgt cggattccac cgcgagcatg ggccgccgac cgttgcccag
    34861 gcaggcgccg atggcggcgg gcaggccgta gcccatggcg cccaggccgg aggtgaggaa
    34921 gctgcgctgg ccggccttgt tgcggaagcc ggcgtagaag aattcgatgg ccagaccgga
    34981 gctgccggtg gcgatgaggg tgtcgggcgg ggcggcgtcg gacagggctt cgacgaagtg
    35041 ggcgtggccg atggggccgg cgggcgggaa gacgcgtccc tcgttctggg tgtagcgggc
    35101 tttccacgac gcgcagcgcg tccgccaggc gtcgcagggg aggggcggca agtcggcgct
    35161 ggcggcgagg aggccgtcga ggaagtcccg tgcatccacg gcgaggggat gctcgaccgg
    35221 catgggcagc ttggcgagtt cgtggggatc cacgtccacc accaccttga cggcgctgcg
    35281 ggcgaagccg gccgggttgt aggcggtgac gatgttgtcc aggcggcagc cgacgctcac
    35341 cagcaggtcg cagttctgca ccgcgaagtt ggcggcgcgg gcggccacca cgccgggccg
    35401 cccgatgttg gcggggtggt cgtagggcag cagatcgagg gcattccagg tgagcacggc
    35461 ggggatctgc aggcgctcgg tgaactgccg gaaggcctcc gccgcgccgg ccagacgcac
    35521 gccgtggccg gccagcagga gggggcgttc ggcgcgggcg aggaggtcgg cgagggtggc
    35581 gaaatccggc gccggcggga gttcggccgc gggcggcgtc cagcctggca gggtgtccgg
    35641 atccaccggc gcggcctgca cgtcgagggg gatgtccagc cacaccgggc cggggcgccc
    35701 gtcctgcatg cggaacaggg cctcctccag gtgggcgcgg atggcctggg gggcttccac
    35761 ggtgacggcg tacttggtga tggtgcggac catgggcacc acgtccacct cctgcacgcc
    35821 gccctggcgc agggggcggc cgttgaggcg gtccgggcgc ttggcctggc cggagatcac
    35881 cagcaggggg accgagtcga tccacgcgcc ggccaccgga gtgatggcgt tggtggcacc
    35941 agggccggtg gtgacgacgc acacgccgaa gcccgcattg cccgccgcgc cggtgcggcc
    36001 gcaggcttcg gcggcgatgg cggcggcctg ttcgtggtgg cagggcacgg ccgtgagacg
    36061 gggctcgcag gcgaggccgt catccagata catggctccg ccgccgggca gcaggaagac
    36121 gtgatccacg ccgaggtcgg ccaggcggcc catgatccag tcggcgacgc ggagggtcgg
    36181 catggcgctt acatccagta ggtgagccag gtcttgcggt gggtgttctg cgcaatggcc
    36241 gggtcggtgt agtaggagtc gttcctgtcg tgggtggagg agatctcctc gaagacggcg
    36301 ccctcctcgg tgcggaaggt gtggcgtacg ccgcgcgcga cggtgatcac gtcgccggcc
    36361 cgggcgtggc gttcttcccc gtccagccac aggatcaggg cgccgtgcag gaccacgaag
    36421 gtttcctcct tcttgaggtg gtactgctcc gggtggagct ggccgggcag catgaccagc
    36481 agcttcttgc agtattcccg gttcaccacg gtgaccatgg tcaggccgaa ttcgtggaag
    36541 agctccagcc cgtagtggtg ggagatctcc aggtcggact ggcccggcac caccacgttg
    36601 ccggccttga gcagggcctt cacggcggtg acgatctcca gcaccttgcc gcgcagctgg
    36661 tgttcggcca cctgggcctg cagcacgggc tggccggcgg cgatgggggc ggtggtgacg
    36721 aagcgggcgt acttggacca gtcgttggcg gtgagctggc cgtccaccgg cgggaaggcg
    36781 aattccacca tcgtggcgtc gagctcggtg ccggccggga tggcgcaggt ggcgaacacg
    36841 ccccggcgca gggactgcag gctggcccgc tcggcggcgg aggggacgta gcggtcgccg
    36901 gaggtgccac agatggccag ggcgcggcgg gcctcgctga gccacgcgcg gatctgcgcc
    36961 gggttggcgg aataggcgtt ggcggcgtag cgctcggtgg gcaggaccac gtgcttttcg
    37021 aagacgctgg cgcccttggc gagggcgagg ccgaccgcca gggtgttgtc cggcgcctcg
    37081 tgggtggagt agccgatgcg cacgccgggg tagcggctgc gcagcagctc gatctggttc
    37141 acctgcaggc tggcgtcggg cgtggggtat tcggcgacac agtgcatcag ggtgaagtcc
    37201 ttgccccggt gcaggaagaa gctggtgacg ttgtcgattt cttccagagt ggcgccggcg
    37261 gtggaggcga tgaccggctt ggcgctggtg gccatgcgct ccagcagggg ccagtctgtg
    37321 aaggagcagc tggcgatctt gagggtgtgg atgccgtggg cctcgatgcg gtccaccgag
    37381 ggctcgtcga agggcgtgca cacggtgcgg aagccgcagg cgtccatctc ggcgacgagg
    37441 gcgcggaagg cctcgtcgga caggcgcgtc tcctcgaagc gcttgacgta cttgatgtcg
    37501 tcgcggccgc gggcggaggc gtggatgaag gtgtccaggt cccggtactg gagcttgaag
    37561 gcgaagtcga agacgtcctc gaagccggtg caggcctcac gcagggcgcg gatggtggcg
    37621 aggccatggg gcaggtcgcc catgtggttg ttggccattt cgaagaccat caggggcttg
    37681 ttcatagatc gactccgagg aagcagcgga tgtggcgggc gatgtggtcg agctgggcgg
    37741 tgtcgaggcc cggccatacg ccgacccaga aggtgtcgtg gaggatgcgg tcggccaccg
    37801 gcaggtcgcc gacgacgcgg aaggtccgcc cggccatgga gggctggcgg atcaggttgc
    37861 cggcgaagag caggcgcgtg ccgatcctgt gctggtccag gtactggagc aggtcgacgc
    37921 ggtggacgcc ggcctccggg cgcagggtga ggggaaagcc gaaccaggcc gggtcgctgc
    37981 ctggcgtggc ttcgggcagt atcaggacgt cggcggcgtc ggccagggcg gcgttcaggt
    38041 aggcgaagtt gcgcttgcgg gcggcgatga aagcgtcgag ccgatccagc tgggcgaggc
    38101 cgcaggcggc ctgcatgtcg gtgatcttga ggttgtagcc gatgtggctg taggtgtatt
    38161 tgtggtcgta gcccgtgggc aggctgccca gctgccagcc gaagcgcttg ccgcaggtgg
    38221 cgtcgcagcc cggcttgcac cagcagtcgc ggccccagtc gcggaaggat tcggcgatct
    38281 ggcgcagttg cgggtcgctg gtgagcaccg cgccgccttc gcccatggtg atgtggtggg
    38341 cggggtaaaa ggagagggtc gccaggtggc cgaaggtgcc gacgcggcgg cctttccacg
    38401 tggagccgag ggcgtcgcag cagtcttcca ccagccacag gccgtggcgt tcggcaaagg
    38461 cgcagacggc gtccaggtcg aaggggttgc ccagggtgtg ggcgatcatg atggccttgg
    38521 tgcgcggcga cagggccgcc tccagcatcg tcacgtcgag gttgtagccg ggcagggcgg
    38581 catccacgaa gaccggcacg gcgccgttct ggaggatggg attgacggtg gtggggaagc
    38641 cggccgcgac ggtgatgacc tcgtcgccgg ggcggatcgc ccgttcgccc aggcgtggcg
    38701 aggtgagcgc cgagaaggcc accagattgg ccgacgagcc ggagttgacg gtgagggcgt
    38761 agcggctgcc gacgaaggcg gcgaggcgcg cctcgaaggc gtcgttgaag cggccggtgg
    38821 tgagccagcc gtcgagggcg gcgtcgatca tggcgttgcg ctcggcgtcg ccgatgacct
    38881 tgccggaggg cggcaccggg tccaggtcgg gccgccaggc ggagggctcg gcgggtgcgc
    38941 gggcggggcg gacgatgact tccaggtcct ggccggccag ctcgggcagg ttcagggcga
    39001 tgcgtccggc ggcgtcggcc tgcacgcgcc ggcgctgcag ggcggtggct gcggccagcg
    39061 ggtcggtgac ggcgaggcgg gtttggggga cgctcatggg cgggttccaa aggcggtgat
    39121 ttcgtcgagg caggcccggc gcatgtcggc gccagtgcgc cacgcccggt accacgcgag
    39181 gctgcggtcc acggcggtct cggcggacca gcgcggatgc cagcccaggc ggcggcgggc
    39241 cttggtgcag tccaggcgca gggcctgggt ctcgtggatg cgcgggccgt cggcgacgcg
    39301 ccagcgggcg tgatcgcccc agccggcggc gaggcgctcg acgatccacg ccacactgcg
    39361 ggaatcggtg tcgtccgggc cgaagttcca gccttcggcc cagggcaggc cgtccaggac
    39421 cagcttctcg gccagcagca ggtagccagc gaggggttcc agcacgtgct gccaggggcg
    39481 cacggcttcc ggattgcgca gggtgacggc ggcgccggcg gcgaaggcgg cgaggatgtc
    39541 gggcaccagc cgggtggggg tccagtcgcc gccgccgatg acattgccgg cgcgggcggt
    39601 ggccagggcc acgccgcggg gggtgaggaa ggagcgccgg taggccgcgg cgatcagctc
    39661 ggtgcaggct ttgctggcgg agtaagggtc gtggccgccc aggggcgcgt cttcccggtg
    39721 gccccacagg ctctcgtggt tctcatagca cttgtcgctg ctcaccacca cgacggcctg
    39781 cacgcccggc tgcaggcgca cggcttccag caggtgggcg gtgccgaggg cgttgatggc
    39841 gaaggtctcg accggctcgg cgtaggagcg cggcaccaga ggctgggcgg caaggtgcag
    39901 gacgatctcc ggcctggcgg cgtgcagcgc ggcgcagagg gcaggcaggt cgcgcaggtc
    39961 gcccagcgtg gactgcatgc cgcgggcgat gtgggccacg ttgaagaggg cgggctcggt
    40021 gtccggtgcc agggcgtagc catgcacctc ggcgcccagg gcctgcagcc acagactcag
    40081 ccagctgccc ttgaagcccg tgtggccggt gatgaagacc cgccggccgc gccagaattc
    40141 gggattcacc aggttttcca tggggcgttg ccgctggtcc acagttcttc cagtacggtc
    40201 ttgtcgcgca gggtgtccat cgggcgccag aagccggtgt gcgggaaggc ggcgagctgc
    40261 ttgcgggcga tgaggctggc cagtaccggg ccttcgaagg tggtctggtc gccgtcgatg
    40321 gcatccagca ccttgggcga cagcacgaag aagccgccgt tgatgaagcc accgtcgcca
    40381 gcgggctttt cgacgaaggc gctgacgcag ccaccggcgc ggatcttgag ggcgccgaag
    40441 cggcccggcg gttgcaccgc ggcgacggtg gcgagcttgc cgtgttcccg gtggaaggcg
    40501 atctcggcgc cgatgtcgat gtcggccacg ccgtcgccgt aggtgaagca gaaggcgtcc
    40561 tcgtcctgca cgtactggcg cacgcgggcc aggcggccgc cggtctgggt ggattcgccg
    40621 gtatccacca gggtgacgcg ccagggctcg gcgtgacgct cgtggatttc catgcggttg
    40681 tgggtcatgt cgaaggtgac gtccgacatg tgcaggaagt agttggcgaa gtactccttg
    40741 atcacgtaac ccatgtagcc gcagcagatc acgaagtcgt ggatgccgtg gctggaatag
    40801 attttcatga tgtgccagag gatcggccgc ccgccgacgg tgatcatcgg cttggggcgg
    40861 gagacggtct cttcgctgat gcgggtgccc aggccacccg cgagaatgac ggctttcatc
    40921 gcagctccag ggagggatgg agccgatgtt atgcgtggca gggcacggcc ttagggcgga
    40981 taaggcgggg gaaagggggg tatttccggg cggcgcgggg gccgcccggc ggggcgccgc
    41041 ggatcaggcg gcagacggca gatcgtcgat ggcgcggcgg cggcgctgca ggccgagtcc
    41101 ggtgacgccg gtgaggacca gggccacact ggcgggctcc ggcaggcaga agtcgccgca
    41161 ggtgcagcag tcgccggcga agccgggcgc ccgcgcggcg atcaggccgc tgaggccgat
    41221 gccggcgtcg gggatctggg ccatctcgtt gccggtgtcg ccggcggaca ggtaggtcac
    41281 cagggaactg tcgagggcct tgcccggcac gaagtggacg ccggcgaggc tcagcaggcg
    41341 ctgccccgca ctcacctcgc ccggcggcag gccagctgcc ccccggctga ggatgccgtc
    41401 gaggaagctc tggtagctgg ccttcgtgac ggtgggaaat cccggcgtgc catcgaaggg
    41461 cgccaggtag ggcaccagat ccgtctgggc ggcgctgaag cgctgcaggt cgtcattgac
    41521 ccgcgccgcg gccgccagca cctgggcgga gccgcgtccg aaggccgtgt aggcggcttc
    41581 gccctgggcg atgtggacaa atgcgctgac gccgctgctc agcatcgtcg tcatggcgtc
    41641 gccgaagccc gcttcggttg cgttcagggc ctgcaggtct gcccatgtgg tggcgtaggg
    41701 cgcggagccg cggaagtcgc tgagcaggct ggtgatggcc gactggatga ggctctgggc
    41761 gggatcggtc acgacctgcg aggggtcgtc cgggacgagc tccaggctgg gatcccacca
    41821 atgggagatg ccgtaatcga tgagctcgcc cacggcggtg ccggccgtca cgccgacgcg
    41881 cacggcctca ccggtcggcg tgcggatcag gccgctggcg cccacgagca ggccgacggc
    41941 cccgctgtag gacttggccg tggaccagaa gcagctggcc tgggccggca ggctcagggc
    42001 cgtcagggcg accagggccg tgccggcgag ggcgcgcagg gtggggcggg gggctggcgg
    42061 agcacgatgc cgcgagacgg gtcgtgacag gaacgggtgg agcgactgga tgcgcatgct
    42121 gactcctcgg caggaagggg atcgggctgc gcggtccccc gggcgggcgg ccgtgcaggc
    42181 gagctatcct gcgcggcgcc gagttccagt ccggttatgg ccggggttac gacagacttg
    42241 aatgcgggcg gcccgtccgt ccgccgtgca gcgcgggcgc cgcgggggac ttgcgacgtg
    42301 ctcggaggag gtcagttgcg gacggcgaac agggcggcgc agcccacgcg ttcaagcgcc
    42361 acctgccgga aacgctgcct cagcgccgcc tgcagggtgg cgaagtcgtc accggcgttg
    42421 gagaagatgc ccttgcggtt gtaccaggcc atcagcgagc gggccgccgc attgcgcggg
    42481 gcgtcgccct gcagcagggt gctgccgaag agcaccgcgc cggggttgag cagcgggcgg
    42541 atgtggtcga aggcggaaag cttggcggcg aaggggccgg gcaggcagtg cagcacgtaa
    42601 ttgaggctcg ccgaatcgaa ccgccggccg ggcaccggca gtggcgcgag gacgttgccg
    42661 atgacggtct cgggtcggta gcgggcgagg cggcgggcag cgtgggcgag ggccgccgtg
    42721 ctcaggtcca tcagcacgat gcgcggggcg tgcaccggga agcgggcatg gtcgaggaag
    42781 tagccggtgc ctacgccgat gtccaggtgg ttgccgctca cgtgccggtc gaagtgggcg
    42841 cgcaggcggg cggtcgggca cttccacagc agcgggttcg agacgcccat caccacggcg
    42901 tcgtagaggc gcaggctgag gggcgtgtag gcggcgtggg cggccgcgag cggatcggcg
    42961 ggggcggtca tggacggctt tttggagagg cttttcgagg gggcgcggcg atgctcggat
    43021 tgtacgcgtc gggcaggggc gcctccatgc gcaggacatg atgggcggtg tcgaggtgct
    43081 ataccgacgg gatgttctcg cactacactg tgcgccgtgg ccgacatggt cgttcggccc
    43141 tggaaaagga gaagcgcatg agttcttccg agctgcccgt ccgcttcagc gccgccgaaa
    43201 tggaacagat cgaggccgcg ctgacgaccc tcgaacgcct gttcatgcgc tactacatcc
    43261 tcgactccaa ggagcgctac ggcatgggcg aattgggctc caccggcgag gccctgtgcc
    43321 gcaggatgct caagatcttc gcccacgagc ccctcatcat gccggacgac ttcgatctgc
    43381 tggctttccg ccgggatctg gcggccctgg accagctccg ccccatcctg gcccgcctgc
    43441 agacgctcac cgacaaggcc accgacgccg aggcgctgct caacgacgag ctgtccttct
    43501 cggtcttcga cggctacgcg ctggtcaaga tgattggccg gcccgaggat ttcgacgcgc
    43561 tgctggccat catggcgggc accgccaccg aggatttcag cgggccgccg ctctgacgcc
    43621 gcccgaccgg ttcacagata gcgccgtacc cggcgccggt agtccacgta ggccgcaccg
    43681 aagcgggcgt gcagataacg ttcctcgcgg cggatcacgc cccagtgcat cgccaccagc
    43741 agcggcgcaa ggggcagcag cggccagggg ctggcgagca gcccggtgag ccccaggtag
    43801 aggccggtca tggccacgta gatggggttg cgcgaccagc cgaagggccc gtccgtgccg
    43861 agagccgccg aggcctgacg cggacttgcc gaggtgccca tgcgccgcag tgtgacgaag
    43921 gcccagcggg cgaaggcggc actggcgatg aacaggacga ggcccgcccc gcgcagccat
    43981 ccggtcatcg cccaggaggc cgtgggccac agggcctccg ccagactccc ggcgaagaag
    44041 gcccccaggt agagcagcgg cggcggcgcg aggatgcccg cggtcgccgc tgcgccatcg
    44101 gcagtgtcgg tcatggtgcc tcagcccagc tcggccgggg tgttcaccgc gcgccagtgg
    44161 ggcttcaggt cggcgggcgt caggctgccg tagccccagg tggtggcgat gaagggcaga
    44221 ccgttggcgt cggccgattc cccgtcctcg ctgcggtcgc cgatgtacac cgcgccgtcc
    44281 cgcggcacgc cctcgtcggc caccaggcgg gcgatcatcg ccgccttgtc cggcaggcgc
    44341 ggtgtgaagc ggtcgagggc atacaccgca tcgaaatacc cgtcccagcc cagatgcgcc
    44401 aggatcagcc cggtgggatg caggcgcttg ttggtggcga tggccaggta acgtccggcg
    44461 cccttgaggc gcgccagcat ctcgccgacg ccggcatagg cggccgtggc ctggtagccc
    44521 gtggtgtcgt agctggcctt gaaatgcccg gccagggtgt cgatcagcgc ggcgtccgtg
    44581 cggccggcga gcaactgcag ggtctcgacg aggggcgggc cgatcacgct ggcgtcgatg
    44641 gcccgcaccg gcgccacgcc ggcccgcgcg aaggcctcgc ggaaactggc caggatcgcc
    44701 gg
//
LOCUS       DAOQMM010000017        40897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_230_length_40897_cov_51.292566, whole genome shotgun sequence.
ACCESSION   DAOQMM010000017 DAOQMM010000000
VERSION     DAOQMM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 40897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40897
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230_length_40897_cov_51.292566"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PLH85_04750"
     CDS             <1..1511
                     /locus_tag="PLH85_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HNB63899.1"
                     /translation="TTDAAGNSASAASSFVEYLHAPTAPTLTLSTPTVPTLSNGLIRD
                     TWNASVGPYNSGNGVAPTTMEASIDALIAAGTAPNTSTVLTNAAVASVAVGVASETHG
                     LIYLTGGTAYTFQIKGDDSILLEIGGQTVGSVTWGTGSLANGKFTPTTSGWYTLDLYH
                     DNEAGPGNFTVRVSTNGSSFVNLDTTNFQLAQSVQDLNSAGIRTSSLQGSSTTEPNDG
                     YYTVLPYNEGHEAAWIPLSTVAGSLVSPGASDVLNIHITGLAQNTQLTDGTHWATADA
                     SGTVDVTGWTMNALSALAPVDYSGTMNAQVQAIATDTLNGVSTTTNKALAIVVDPYVT
                     VAGTTGTGTDDRIVGTSGADTLKGGEGSDLIIGGAGSDTLTGGAGSDVFRWMLADKGT
                     PGLPAQDTITDWSSAAVKNGGDVLDLRDLLQGESHGASGLGNLTSYLHFEKVGTATVV
                     DISSSGGYSAGFKASATDQTIVLSNTDLTSGGTLNDAQIIQDLMTKGKLHTD"
     gene            complement(1659..3473)
                     /gene="typA"
                     /locus_tag="PLH85_04755"
     CDS             complement(1659..3473)
                     /gene="typA"
                     /locus_tag="PLH85_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNB63900.1"
                     /translation="MSRSIRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQVSERVMD
                     SNDLEKERGITILAKNCAIQYGDTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLKPIVVINKIDRPGSRPDWVINHTFDLFDKLGATEEQLDF
                     PVVYASALNGYAMLDADQPGTDMKPLYEAILQHVPEPDVDAEAPLQLQICSLDYSTYI
                     GQLGIGRIKRGRIRPNQEVAVMYGDESRGKAKISQILTFTGLDRAPAESAEAGDIVLV
                     SGIDQVNIGVTLCAPESIEPLPPIAVDEPTLTMNFMVNSSPLAGREGKFVTSRQIRER
                     LEKELLKNVALRVNFTSDSDTFEVSGRGELHLTILLENMRREGYELAVGRPRVVFKEI
                     DGVKCEPYEMLSVDVEEEHQGGVMEELGRRRGEMQDMQPDGKGRVRLEYRIPARGLIG
                     FQGEFLTLTRGTGLASHVFDDYSPMAGQMSERRNGVLISQDDGDAVAYALWKLQDRGR
                     MFVSPGDALYEGMVIGIHTRDNDLVVNPIKGKQLTNVRASGTDEAVRLVPPIQLTLES
                     AIEFIADDELVEITPKNIRLRKRYLKEHERKRAARADA"
     gene            3604..4341
                     /locus_tag="PLH85_04760"
     CDS             3604..4341
                     /locus_tag="PLH85_04760"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNB63901.1"
                     /translation="MLAIIGGSGLTQLANLEVLRREVVRTPYGEPSGALTFGRLCANP
                     VVFLARHGYGHTIPPHRVNYQANLWALQQAHVTSIISVASVGGIRADLGPGALVVPDQ
                     IIDYTWGRKSTFFEGGDAPVVHVDFTEPYDAGVRQRILAAAVAAGEAVSDGGVYAATQ
                     GPRLETAAEINRLERDGADVVGMTGMPEAVLARELGIPYAAINVVANYAAGRAGSANG
                     ICFDSIEQVLLGAMGRVRRVLDHLCDA"
     gene            4338..4871
                     /gene="def"
                     /locus_tag="PLH85_04765"
     CDS             4338..4871
                     /gene="def"
                     /locus_tag="PLH85_04765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB63902.1"
                     /translation="MIRDVLRMGDPRLLAVARPVEAFDTPELRGLIQDMRDTMTALNG
                     AGIAAPQIGVDLRVVIFGGGGPNPRYPDAEFVPDTVLINPQIELLSDEEEGAWEGCLS
                     VPGLRGWVPRRVHLRYRGVDMEGRPFEREARGFHARVVQHECDHLDGILYPARVRDFS
                     RFGFTDVLFPGVPLADD"
     gene            complement(4896..>6779)
                     /locus_tag="PLH85_04770"
     CDS             complement(4896..>6779)
                     /locus_tag="PLH85_04770"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNB63903.1"
                     /translation="EEATELREVERFLQHVRIRLHHLTGRREDRLLFDHQEKLARRFG
                     YEATGTRRASEVFMQEYYRSAKKVTQLNTILLQNFGAELFPNRYPIAININERFQCVR
                     ELLDVRDEGVFERHPGALLECFLILEQRSELKGMTARTLRALWRGRKLINADFRRDPA
                     HRALFLRILQQKRGVLHEVRRMNQYGILSFYLPAWRRIVGQMQHDLFHVYTVDQHILQ
                     VLRNVRRFMVADHAHEYPLLTRLATSFEKPWLIYIAALFHDIAKGRGGDHSKLGMKDA
                     REFCESHGLDDTDTGLVEWLVEHHLSMSNVAQKHDLSDLSVIQRFAALVGDERHLTAL
                     YILTHADIRGTSPKVWNGWKGKLLEDLFFATQRLLRGVAPQQALGSPKRQDDARHLLR
                     YYGLRPGTEDALWAQLDTLYFLRHDAEEIAWHARMLYYRVETDQPVVKARLSHVGEGL
                     QVMVYMHDQRDLFMRLCGFFARLGFTIADAKIHTTRHGYALDSFVLLDPGQELQYRDI
                     TNLLEHDLTERLRTQAPADQPTNGRLSREVRHFPITPEVSIRPDDRGKHYIMSVTAAD
                     RTGLLFDIADTLAHYSINLHTAKIATLGERVEDTFLISGPGLSQDNLVVKIESDLLEK
                     LAI"
     assembly_gap    6780..6879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6880..7343)
                     /locus_tag="PLH85_04775"
     CDS             complement(<6880..7343)
                     /locus_tag="PLH85_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNB63904.1"
                     /translation="MSQTESPALLSFEQSRELITRIKTRLKSGAETLRAAYEAHPLTE
                     PILKGRCQLVDEVLVELWQIFQLPDSAALVAVGGYGRGELFPCSDVDLLFLLDAPADP
                     ELSSRLEQLIGLIWDIGLDIGHSVRTVEECLEESARDVTVTTNLLEARLLHG"
     gene            complement(7345..8166)
                     /gene="map"
                     /locus_tag="PLH85_04780"
     CDS             complement(7345..8166)
                     /gene="map"
                     /locus_tag="PLH85_04780"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNB63905.1"
                     /translation="MSVTIKTPEEIEKMRIAGRLGSEVLDYITPFVKPGVTTGELDKL
                     CHDYMVDVQGCIPAPLNYAPPGYKPYPKSICTSINHQVCHGVPGEKALKKGDIVNLDI
                     TVIKNGYHGDTSRMFIVGGEAAGSILARRLCQITFECLWLGIAQVKPGAHLGDIGHAI
                     QKHAEGHGFSVVREFCGHGIGAKFHEDPQVLHYGKPGTGLELKPGMIFTIEPMINAGK
                     AAISELPDGWTIVTRDRSLSAQWEHMILVTETGVDILTLSEGAPRRPELVAHLFP"
     gene            8465..9217
                     /gene="rpsB"
                     /locus_tag="PLH85_04785"
     CDS             8465..9217
                     /gene="rpsB"
                     /locus_tag="PLH85_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNB63906.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMAPFIFGQRNKIHIVNLEK
                     TLVKYNEAMDFVRKLAANRGTILFVGTKRQAREIVAEEARRAGMPYVDERWLGGMLTN
                     FKTVKVSIKRLKDMEAMVEDGSIERLSKKEALMASRELEKLQKSLGGIKDLGGLPDAL
                     FVIDVGYHKIAITEANKLGIPVVAVVDTNHTPEGVDFVIPGNDDSSRAIRLYARGVAD
                     AVLEGKNQVVQEIVAAGSDEFVEVEEGNEEQA"
     gene            9278..10174
                     /gene="tsf"
                     /locus_tag="PLH85_04790"
     CDS             9278..10174
                     /gene="tsf"
                     /locus_tag="PLH85_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNB63907.1"
                     /translation="MAEITASMVKELREKTDAPMMECKKALAEAAGDMAKAEEILRVK
                     LGNKASKAAARVTAEGIVGVYIGEGGKLAAIVEVNCETDFVAKNDDFLALTKKLAELV
                     AAANPADVEALSALTIDGQSVEEVRKALVGKIGENMSIRRFVRTEAKGAVASYIHGGA
                     KIGVLVDLVGGDEALAKDIAMHVAAAKPKALDASGVPSELLDVERRIAIEKAREAGKP
                     EAMLEKIAEGTVQKYLKDVTLLDQVFVKAEDGKQTIAQLLKAKGASVAGFTLFIVGEG
                     IEKKVTDFAAEVAAQAAAAAQK"
     gene            10190..>10380
                     /locus_tag="PLH85_04795"
     CDS             10190..>10380
                     /locus_tag="PLH85_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNB63908.1"
                     /translation="MSQPAYKRILLKLSGEALMGDDAYGINQDVVTGIVTDVAEVVQL
                     GVEVGIVIGGGNIFRGMAGA"
     assembly_gap    10381..10480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10481..10768
                     /locus_tag="PLH85_04800"
     CDS             <10481..10768
                     /locus_tag="PLH85_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNB63909.1"
                     /translation="TAAALRGAEIGANIVLKATKVDGIYTADPKKDPTATRFQRISFD
                     EAISRNLAVMDATAFALCRDQRLPINVFSLFKVGALKRVVMGEDEGTLVYC"
     gene            10779..11336
                     /gene="frr"
                     /locus_tag="PLH85_04805"
     CDS             10779..11336
                     /gene="frr"
                     /locus_tag="PLH85_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNB63910.1"
                     /translation="MIPELKKTTEHKMLKSIEALKADLAKVRTGRAHTGILDHVMVEY
                     YGNPVPVNQVANVTLIDARTIGVQPWEKPMVGKVEKAIRDSDLGLNPAAQGDIIRVPM
                     PPLTEERRKELIKVVKHEGEGAKVAVRNLRRDANSSLKDAVKAKTISEDDERRAQDDI
                     QKLTDKAIVEIDKLLVQKEQELMQI"
     gene            11380..12168
                     /gene="uppS"
                     /locus_tag="PLH85_04810"
     CDS             11380..12168
                     /gene="uppS"
                     /locus_tag="PLH85_04810"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNB63911.1"
                     /translation="MVRLPFLSSTRGIPEVVEVPRHIAIIMDGNGRWARKRLMPRVAG
                     HAKGVESLRAMIRTCQDLKVEYLTVFAFSSENWRRPKDEVTFLMKLFLRTLKEEIERM
                     HRSNIRLRVVGDISRFDSALVNLIEESETLTAGNTAMNLTIAANYGGRWDILQAVEKM
                     LAASPDAREGFTEDQLAEYLALSFGPEPDLFIRTGGEQRISNFMLWQLAYTELYFTDK
                     LWPEFDEAALKEAIRSYQGRERRFGRTSEQLDAGAGGPDSSDHA"
     gene            12161..12988
                     /locus_tag="PLH85_04815"
     CDS             12161..12988
                     /locus_tag="PLH85_04815"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNB63912.1"
                     /translation="MLKTRVITALCLLAGILAALFLLPEPAWVVFCALICAAAAHEWA
                     GMIGFAAARRAVYSVLLGALCLAGGCVAGLHRPETVAPFALAPVYGVSALFWLTCVPV
                     WLKSRWQLRGVGAGALVGLVLLLPPSLAIAHLRLLGPWVLLAVMAAVWVADIAAYFSG
                     RAFGSRKLAPSISPGKSWEGAYGACVGVVVYGFACAASVGLDWTAPRVWMPGVIGLMG
                     FTAVSIIGDLFESLIKRQAGIKDSGTLLPGHGGVLDRIDSLTSTLPMAALVALWLAR"
     gene            13001..14197
                     /gene="ispC"
                     /locus_tag="PLH85_04820"
     CDS             13001..14197
                     /gene="ispC"
                     /locus_tag="PLH85_04820"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNB63913.1"
                     /translation="MKKLITVLGATGSIGVSTLDVVARHPDRYGVFALTAHRQVDKLY
                     EQCLAHRPAYAVVERAEDAVALQRRLDAAECRCDVLYGPAALCDVASAPDVASVMAAI
                     VGAAGLPPTLAAARAGKRVLLANKEALVMSGALFMAEVDTHGATLLPIDSEHNAIFQA
                     MPSGHARPLETGGVTQILLTASGGPFRSTPISDLPAVTPDMACAHPNWVMGRKISVDS
                     ATMMNKGLEVIEARWLFAAPAERIQVVIHPQSVIHSLVAYSDGSVLAQLGNPDMRTPI
                     AHALAYPERIDSGVKPLDLFEVARLDFERPDFARFPCLALAYRALQAGGVAPAVLNAA
                     NEVAVAAFLDGHLRFDRIAAVIADVLDAVPDAAADSLEVVFAADREAREVAASRVRAY
                     LAGARP"
     gene            14194..15558
                     /gene="rseP"
                     /locus_tag="PLH85_04825"
     CDS             14194..15558
                     /gene="rseP"
                     /locus_tag="PLH85_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNB63914.1"
                     /translation="MILVDYVLPFLLALGILIVVHEFGHYQVARWCGVKVLRFSLGFG
                     KPLMLWRAGADRTEWAIAAFPLGGYVKMLDEREGQVPTEELHRAFNRQNVWKRIAIVA
                     AGPLANFLLAITLYWGLYGGGVEEIRPRVGTPVAGSQADLAGFQDGELVRAVSGEQVQ
                     TWQEFRWKLLNRALEHQAVEVETQNGQGEIAFRRLDLAGITVDQGTEDITAQLGMKLF
                     RPVLPAVIGSVAPDSVAARAGLQGGDRVLRIDDKDIGGWSELVMMVRDAPGRRLRFEV
                     VRGTEALAVDVVPAAVEDRGRTIGRIGVGLAENAQRRDEMFVTVRYGIVDALGKGLSQ
                     TWETTVMSLSVMRRMIVGEVSVKNLSGPVTIADFAGQSARMGLPHYIKFLALISISLG
                     VLNLLPIPLLDGGHLLYYVLEIIKGGPVSDRTMEIGQQIGMALLAMLMAFAFYNDINR
                     LVAS"
     gene            15565..17856
                     /gene="bamA"
                     /locus_tag="PLH85_04830"
     CDS             15565..17856
                     /gene="bamA"
                     /locus_tag="PLH85_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNB63915.1"
                     /translation="MNSKLLPAVIAALICASPALAFEPFVVKDIRVEGIQRTEAGTVF
                     NYLPVKVGERFSEDKASQAIKALFATGFFKDVRIEVDGDVIVVVVDERPAIAQVDFVG
                     IKEFDKEALKKGLKEVGLAESRIFDRAVLERAEQELKRQYLSKGLYGVQITTTVTPLE
                     RNRVGVNFNVVEGDAAKIRQISIIGNKVFKEKDLLDLFALTTPGWMTWYSKSDQYSKQ
                     KLSGDIEALRAYYLNRGYLDFTVDSTQVSITPDKKDIYITLSITEGEKYTVSDVKLAG
                     DLVLPEKEVLNLVKVKSGDTFVRDNVTATTKAISDRLGNEGYAFANVNAAPEVDKVKR
                     QVAFTFYVDPGRRVYVRKINFAGNARTRDEVLRREMRQMEGAWYDGAALNRSRTRLDR
                     LGYFDEVTVETPAVPGSTDQVDANFSVKERATGNLMLGAGFSSSEKVILSGSISQQNL
                     FGTGNSMTLQLNTGRINRTAALSFTNPYWTIDGVSIGWDVYQRNVDPTSLSVASYKTH
                     SLGAGLRFGYPIAEDDRINFGLAVDRTKITVFDTSPIQYVDFVNKFGSTAESLVSTVG
                     WSRDRRDSFVYPTSGTYQRVGLEMATAPMDIRYFRSSYQHQHWIPFGGGYALMLNGDV
                     GYAHGYDGKELPFYKNFYAGGIGSVRGYQQSTLGPQFVDVYGVARALGGNRRIIGNAE
                     YYFPMPGQGKEKSMRMSMFLDAGQVWGVEEKVRLSDLRYSAGLAFSWSSPVGPLKFSL
                     GQALKKEPQDRTQKFQFQLGTVF"
     gene            17886..18389
                     /locus_tag="PLH85_04835"
     CDS             17886..18389
                     /locus_tag="PLH85_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB63916.1"
                     /translation="MKGIGISTLAGVLLAVFAQTAAAEVKVGFVNSDRIMKEAVPAKK
                     AQQKLEKEFEKRDQDLQRMAKQLQGMQEGLEKNGVTMAEGEHRAKEREFNDLNRDFQR
                     KQREFREDLNQRRNEELAGVLERANKTIKAIAEAEKFDIIFQEAVYASPRIDITDKVI
                     KALTETK"
     gene            18402..19433
                     /gene="lpxD"
                     /locus_tag="PLH85_04840"
     CDS             18402..19433
                     /gene="lpxD"
                     /locus_tag="PLH85_04840"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNB63917.1"
                     /translation="MQITLGALVERLGGELIGDPSIVIRQIATLEKAGEGDLAFLANP
                     KYRAALDGTTASAVIVDPKSAGLTERARIVTRDPYLYFARAAQWFNPLRRPPAGIHAS
                     AVVASALPESVSVGPNAVIGEGCEIGENTVIGPGCHVGDGVRIGPDGLLHAGVTIYAD
                     CVVGARAILHSGAVIGADGFGFARDSDKRWVKIPQIGRVVIGDDVEVGANTTIDRGAL
                     DDTVIGNGVKLDNLIQIAHNVHVDDDAIMAGLSGIAGSSRLGKRVMVAGQAGISGHLS
                     VGDDIVVSAGTIITKTLDKPGVYTAMLPQMTHADWVKNVSHVRRLDGLADRVRELEKL
                     LAEKNKENS"
     gene            19439..19870
                     /gene="fabZ"
                     /locus_tag="PLH85_04845"
     CDS             19439..19870
                     /gene="fabZ"
                     /locus_tag="PLH85_04845"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNB63918.1"
                     /translation="MDINEIKQYLPHRYPFLLVDRIIELELDKRVVGIKNVTINEPFF
                     PGHFPTNPVMPGVLIVEAMAQTAAVLAFKSDGSVPDEHSTVYFASLDNVRFKRPVVPG
                     DQLVMEAEIVRKMRNIWKFRAVARVDGELATEAEFMCAIKR"
     gene            19867..20640
                     /gene="lpxA"
                     /locus_tag="PLH85_04850"
     CDS             19867..20640
                     /gene="lpxA"
                     /locus_tag="PLH85_04850"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNB63919.1"
                     /translation="MSIHPTAIVHPGARLAEGVQVGAYSIVGEHVVIGEGTSVGPHVV
                     IEGHTTIGCNNRIFQFCSLGSEPQDKKYAGEPTRLEIGDGNTIREFCSFSTGTVQDGG
                     LTRVGHDNWIMAYVHVAHDCVVGDHTIFANNATLAGHVTVGDWAILGGFTGVHQFVRV
                     GAHSFCGVGTVLLQDLPPFVTVSGNPAAPHGINSEGLRRRGYSSDGITAIKRAYKTLY
                     RSGLTLDEARRQIAEAAANVPELQMFSDFIADSGRGIVR"
     gene            20644..21792
                     /gene="lpxB"
                     /locus_tag="PLH85_04855"
     CDS             20644..21792
                     /gene="lpxB"
                     /locus_tag="PLH85_04855"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNB63920.1"
                     /translation="MTVRIAMVAGEASSDLLASHLIQALRRHLPDAEFYGIGGPKMQA
                     LGFQALWPAEKLAVHGYVDALRHYRELSGIRRQLKARILADRPAAFIGVDAPDFSLWL
                     ERRLKDKGIPAIHFVSPSIWAWRRGRLKGIARAVSHMLCLFPFEPELYRTAGVPVSYV
                     GHPFADVLPLAPDRAGARELLQLSGTGPVFALLPGSRQSELRNLADTYIATAAILHER
                     HPDATFLVPLATRETRLLWDAAMARHAHLTLPLRTLFGHAVEAMTAADVVLVASGTAT
                     LEAALLKRPMVITYRIGKWSWRLMKRLAYLPWVGLPNVLAGREVVPELLQDAATPQAL
                     ADALDAWLQDAGRRGELLETFTDMHRQLRQDTASRAAEAILPYLRGRV"
     gene            21792..22388
                     /gene="rnhB"
                     /locus_tag="PLH85_04860"
     CDS             21792..22388
                     /gene="rnhB"
                     /locus_tag="PLH85_04860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNB63921.1"
                     /translation="MERLCGVDEAGRGPLCGPVVAAAVILDPARPIAGLADSKKLSAA
                     RREALARTIKAQALAWHIAEASVEEIDRLNILHASMLAMQRAVAGLNLRPTRVQVDGN
                     RCPALDLPCEAVVRGDSLVAEISAASILAKTARDALLVALDRRYPQYGLAGHKGYPTA
                     AHLAALKAHGACDIYRRSFGPVRAVVAQASLWNEDPHP"
     gene            22385..22840
                     /locus_tag="PLH85_04865"
     CDS             22385..22840
                     /locus_tag="PLH85_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNB63922.1"
                     /translation="MKVHHLLLFLHLAGVIVWMGGMVFAHLCLRPAALELPPAQRLSL
                     WRAVFGRFFPLVAAAVATILLTGFAMLADVGFAQAPRTWHVMALLGTVMAGVFASIWF
                     GPWPRLRQAVAEESWARGAEALATIRQRVGLNLVLGTLTVAVAILGLAL"
     gene            22846..23640
                     /locus_tag="PLH85_04870"
     CDS             22846..23640
                     /locus_tag="PLH85_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB63923.1"
                     /translation="MKRLTSRDNAAVRHLHALATSARERRKAGETLLDGAHLVQVALS
                     RGQSLRQIVVSDAGFAQAEIATLLAAAPDVAQLVLPDALFAHVSPVDSPSGVLAVMAL
                     PAGQAGNSPTGDCVLLDGVQDPGNLGTILRTAAAAGIAQVLLTPGCAQAWSPRVLRAA
                     MGAHFLLHIDEGIDASAALAGYPGAILATALQDATELFATDLRGPVAWLFGSEGQGLS
                     EGIAALATGRVRIPMPGGMESLNVGVAAGVCLFEQVRQRGLHPSGG"
     gene            complement(23657..24322)
                     /gene="bioD"
                     /locus_tag="PLH85_04875"
     CDS             complement(23657..24322)
                     /gene="bioD"
                     /locus_tag="PLH85_04875"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNB63924.1"
                     /translation="MSAYFITGTDTEIGKTFSTCALLHAARAQGLRALGMKPVAAGAE
                     WVNGEFLNEDAARLRAAGSFDPGLALLNPYCLASPIAPHIAAREEGVRIEPARIRTAF
                     DTLRAQADLLLVEGVGGFRVPLGDDYDSADLARDLGLPVILVVGLRLGCINHALLSVE
                     AIAARGLTFAGWIANRVDPAMLRVDENLAALQKGLPAPLLGVLPFREDRDAALAATAL
                     RLP"
     gene            complement(24319..25092)
                     /locus_tag="PLH85_04880"
     CDS             complement(24319..25092)
                     /locus_tag="PLH85_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB63925.1"
                     /translation="MTDAPASKRAVRAAFDRAAAAYDSVAHVQRSACDRLFALGTPHL
                     STAARVVDAGCGTGYALPGLRQRFPDAELCAVDFAPAMLARLPAGLAHPLCADLEQLP
                     VADACVDLLWSSYALQWCAPRRALTDIARVLRPGGQVWLATLGPGTLFELRDAFAAVD
                     DAEHVLRFQPPEVVEDAAESAGLRLLASERDTLQAWAPDLRRLLHDIKTLGAHQTGAT
                     RRPLRKTDLARLTAAYEAYRTPDGLPASYDTLWLVAEKP"
     gene            complement(25085..25813)
                     /locus_tag="PLH85_04885"
     CDS             complement(25085..25813)
                     /locus_tag="PLH85_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB63926.1"
                     /translation="MSALVLLHGWGLSHRVWAPLLAALPGHAMLTPDLPGHGDAPRAG
                     PRLADWSDAVAAGLPKGCTLAGWSLGALLALDIARRHPDKVARLLLTGASPRFTATDD
                     WPCALAADTLRDFRTAFAAEPDATQRRFVALQALGDHARRNVTQTLTAALTPADTAHR
                     EALADGLALLADTDLRPILPAIAQPVRLLHGARDALMPVAAAEHLADALPHARLSIFA
                     DSGHAPFLSRPDDFATLVEGARHD"
     gene            complement(25810..26994)
                     /gene="bioF"
                     /locus_tag="PLH85_04890"
     CDS             complement(25810..26994)
                     /gene="bioF"
                     /locus_tag="PLH85_04890"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNB63927.1"
                     /translation="MPAEAHDPLDALATGLAELDAAGLRRRRRVVASPCAPAATLADG
                     SARIAFCSNDYLGLASDPALIDAARAGAARWGVGSGAAHLVSGHTVAHDALEARLAAF
                     TGFDAALCFTTGYMANIGVMPALVGRGDAVFADRLNHASLVDGALLSRADLHRYPHGD
                     VDALARQLTASSAPRKLIVSDGVFSMDGDIAPLPRLLELAETHKAWLLVDDAHGFGVL
                     GPQGRGSLAHFGLRSPRLIYMGTLGKAAGVAGAFVAARADVIDWLLNKARSYIFTTGA
                     PPLLADTLLAAVDLIEQGDARRAHLSALIQRLRNGLTLRRWHLMPSTTPIQPLVIGDN
                     HEALAVARALDEAGLWVPAIRPPTVPKGSARLRITLSAGHTIEHVDHLVTTLRRLEAG
                     AP"
     gene            complement(26984..28306)
                     /gene="bioA"
                     /locus_tag="PLH85_04895"
     CDS             complement(26984..28306)
                     /gene="bioA"
                     /locus_tag="PLH85_04895"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNB63928.1"
                     /translation="MSNAALLARSLAAVWHPCTQMKHHEQLPLIPIARGEGPWLYDMD
                     GRRYLDGISSWWVNLFGHANPRIGHAIKDQLDRLEHVLLAGFTHEPVVELSERLAALT
                     GHRLGHAFYASDGASATEIALKMSAHFWRNQGQPHKNRFVCLAGSYHGETVGALAVTD
                     VALFRDAYAPLIRPASCVPNPDARQAMPGETAVDVARRAAAALETFLAEHAGETAALI
                     VEPLVQCASGMAMYDPEYLRLARALCDRHQVHLIADEIAVGCGRSGSFFACEQAGITP
                     DFLCLSKGISGGYLPLSIVLTRDAVYNVFYDNDTARGFLHSHSYTGNPLACRAALATL
                     DIFAEDEVIAANRHRATRLGEAFRAAVGDHPRVRHLRQQGMILAFDVADAPPDFARRY
                     FAEALQRELLLRPIGPTVYTLPPYILDDVHIDFLARRSAEALEAALAG"
     gene            complement(28400..29653)
                     /locus_tag="PLH85_04900"
     CDS             complement(28400..29653)
                     /locus_tag="PLH85_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63929.1"
                     /translation="MSLRPAFLSRRRLVVGALLGSVILAGCGGKGSSANAPTGGITVT
                     PGDTQVTITWQQDAGVEYWLWYAPGSTINPSDSNTPGIRYHINIGSPYVVTGLTNGTQ
                     YAFALNGRINGGKGGDYAYGTTTPRLAGTTWTAVTNLTADMRGLAYGTTSTVTTATYV
                     AAGTAGAAFSSTDGHTWTALANPAGTANVNAMTVGLSTFIAVGDGGAIYYSTDLSTWT
                     QATSNTTNKLTAVATSGGVAVAVGENGTIRYSTSAGTWTAAASVPSGIGHLYGISYTP
                     SGLWIAVGAGGVVLTSSDGTNWTQRTSGTAATLYGVSGMATTSGSTTTYTYVATGAGG
                     QVLTSSDGTTWTASTVSPAVTLYSVIGSNQFVAVGANGAAFTSPDGVTWTYYAASSGN
                     DLYTLMKAANQYIAVGANKAMAYSQ"
     gene            complement(29758..31842)
                     /gene="ppk1"
                     /locus_tag="PLH85_04905"
     CDS             complement(29758..31842)
                     /gene="ppk1"
                     /locus_tag="PLH85_04905"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNB63930.1"
                     /translation="MRPAPAPRYSTDHFINRELSLLQFQRRVLAQAADPAVPLLERLR
                     FLCIVSSNLDEFFEIRVAGVKEQMRLGRVATTADGTSPRVLYEHISREVHSIISEQYA
                     LLNDTILPALAAEGIVFLRRVAWTEAQHAWARDYFLREVMPVLTPIGLDPAHPFPRVL
                     NKSLNFAVELEGRDAFGRNSGAAIVQAPRALPRVIRVPRELADAEYTYVFLSSLLHAH
                     VGTLFEGMNVQGVYQFRVTRNSDLYVDEEEVKDLRATLKGELQQRHFGDGVRLEVADN
                     CSSAMADFLKSQFGLGDADVYRVPGIVNPVRLIQVPDWVDRPDLKYRPFLPARPKGLD
                     KRDNIFAAIRRQDILLHHPFQSFEPVIELLQAAADDPQVMAVKMTVYRTGTDSVLMEH
                     LVRAAQKGKEVTVVLELMARFDEEANISWAARLEEAGAHVVYGVFGYKTHAKMLLLVR
                     REDRGLKRYVHLGTGNYHPRTTRFYTDFGLLTANEEIGADVAEVFKQLTGLGQAGKLR
                     HLWQAPFTLQPNVVAAIRNEAEIARAGRKARIVVKVNALLEAETIEALYDASCAGVDI
                     TVIARSVCALKPGVPGLSENIRVRSVVGRFLEHHRIFYFHADGAEKLYLSSADWMERN
                     FFKRIEVAFPILDPRVKRRVVKEGLRPYLGDNCQAWELDQDNHYRRKNPRTSRRAAQE
                     LLLKDVAGVTLP"
     gene            31993..33495
                     /gene="ppx"
                     /locus_tag="PLH85_04910"
     CDS             31993..33495
                     /gene="ppx"
                     /locus_tag="PLH85_04910"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNB63931.1"
                     /translation="MNHELIAAIDLGSNSFRLQVGRIVNDQIYPLDGIKESVRLAAGL
                     SPDRMLDAAAQFRGLNALRCFNERIRDYPPEAVRAVATNTLRVAKNAPHFLIQAEAAL
                     GFPIEVIAGREEARLIYVGVAHTLPNPHRQQLVVDIGGGSTEFIIGKSFKPLELESLY
                     MGCVGYSLRYFPDGRVDKKGLREAELAALKEIQTISHAYRRTGWEEAVGSSGSAKALV
                     EILELNGLSAGGITRDGLEKLKVLMLKAGSVQALQLEGLRPDRLPVLPGGFAIMSAIF
                     QEFGLERMVFSEGALRLGVLYDLLGRYHHHDLRDATVTAFMERYSVDVAHARRVADTA
                     LHFLEQLDPSTEDPENTDRRFVEWAALLHEVGVSVAHSSYHKHSAYILGNADMPGFSR
                     MDQGRLSRMVLAHRGKLARVSALDPESLEWRFILCLRLAAVVHRARDGRGVPPLRVKR
                     EGRAYVLLADGDWLRNLPLTAAALDEEERQWQGIGMPLRVRVERAARGAA"
     gene            33521..34651
                     /locus_tag="PLH85_04915"
     CDS             33521..34651
                     /locus_tag="PLH85_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB63932.1"
                     /translation="MNAAPQAAPALRVDGHGAAATVTLTGDWTLRALLPRLSVVQAAL
                     AEVHGVAVWDLDGVIRLDTFGATLLWRHWGRQRPARLQVPTILDENFVRLAALGGPSV
                     PVAPPYRFLDATAHLGRLAIGFGRHLADFTALVGQILLDALHLLGDRQRWPWREMSAN
                     FYKVGVKAMPVTALVGFLIGVVLSYLSALQLRAFGADIYIVNILGLGIIRELGPVLVA
                     VLVAGRSGSAMTAQLGVMRVTEEIDALATMGISRYVRLVLPKVVALFLAMPLLTLWCS
                     AVAIVGGMVSAWLQLGIGFEYFLQALPRAVPFPNLVIGLSKGAAFGALIALVACHFGL
                     RVKPNTESLAAHTTASVVTAITLVILTDAVFAIATRSIGVPG"
     gene            34648..35424
                     /locus_tag="PLH85_04920"
     CDS             34648..35424
                     /locus_tag="PLH85_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB63933.1"
                     /translation="MSAFPVEVKGLSTRFGDTWVHRGIDLAVPAGEIVALVGGSGSGK
                     TTLLRQIVGLLRPTAGEVRLFGEPLFGGSRLEQVARRRRFGMLFQQGALFSALDVYAN
                     IAFPLQEAGVRDEAMLHDLVGVKLAMVELAPEVGLRMPAELSGGMVKRVALARALALE
                     PELLLLDEPTAGLDPDRSAGFVRLIRDLHRALGLTVVLVTHDLDTLAALATRVAVLAE
                     GRILGFGSLDSVMALEHPFIERFFRGERSRAALARGRAEA"
     gene            35424..36368
                     /locus_tag="PLH85_04925"
     CDS             35424..36368
                     /locus_tag="PLH85_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNB63934.1"
                     /translation="MEDRAHAIAAGLFALLLGTALVFTLWWFSDKREVTRDVVLVSAG
                     SVNGLNPQATVRYRGIMAGKVASIALDPARPQDLLVTARIRADLPLTHGTRARLATQG
                     VTGIAFIALDDSGADPTPLAADGNGPPRIALEPGLVEEVADATRRTLGQLRELSERFA
                     RLASNENIGRVERILAHMESSSKGLDKGMQDAAASLAAVHRMLGKDNLARIERTLANA
                     ERFSSDAAPLAADGRRLLARMQDVADRLDVIAASTGEGFATSTLPRLNVLLQELTTTS
                     RQMNDLLDELDSHPQIFLLGRGKAPPGPGEAGFVPNRP"
     gene            36376..37005
                     /locus_tag="PLH85_04930"
     CDS             36376..37005
                     /locus_tag="PLH85_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNB63935.1"
                     /translation="MTHSLRACLSASVSLVVALVSLAGCGAVLPKGAPTFAVHDFGPL
                     APVGPRSLPYPIRTLEVIPAPWLASTAMQYRLAYAQETRRQAFVQSRWAAQPGQLLEV
                     ALKRSVRVNVTTVSLGGCRLRVDLDEFMQVFDNESGSRGVIDARASLLAPRTDQLLAT
                     QGFSVTRPASSPDAAGGVVALRDAVQAFDAALLTWLDGLAGSVGARCSQ"
     gene            37074..37874
                     /locus_tag="PLH85_04935"
     CDS             37074..37874
                     /locus_tag="PLH85_04935"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNB63936.1"
                     /translation="MSAVVDTPQEPMLLRTDADGVTTLTLNRPGQFNALSQAMLEALL
                     AELERIAEDSAVRVVVLAGAGKAFCAGHDLKEMRGNYTKSFQQALFRLCGKVMMKIVA
                     MPQPVVARIHGIATAAGCQLVSMCDLAVASDVARFAVSGINVGLFCATPSVGLARNMG
                     RKEAFEMLVTGDFIDAQEARRRGLVNRVVPLEQLDDEVAKLTASICAKSPAAIAMGKR
                     MFYAQLEMGLDAAYQMAAEAMACNMMCEDAAEGIDAFMAKRTPVWTGR"
     gene            37885..38649
                     /locus_tag="PLH85_04940"
     CDS             37885..38649
                     /locus_tag="PLH85_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB63937.1"
                     /translation="MLIDTHCHLDAAEFADDRSAQIDAARAAGVGRFLVPAVDAGNLD
                     AVAALSAAHADCRHAFGIHPMYVARAQADDLERIRSRLADGSAVAVGEIGLDAYVPDG
                     DLPRQTEFFEAQLRLARTFDLPVVLHIRRAQDAVLGALRRIRVRGGIAHAFNGSRQQA
                     EAFIGLGFKLGFGGAMTYSGSRRIRELAATLPLDALVLETDAPDIPPAWAARQRNLPA
                     NLRRIAAELAALRGMDEAELIAATGRNAVAVIAGLR"
     gene            complement(38659..39243)
                     /locus_tag="PLH85_04945"
     CDS             complement(38659..39243)
                     /locus_tag="PLH85_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB63938.1"
                     /translation="MEKTTRPQLDRAAWIQAALEVLADEGVQGMRVEVLAKRLRVTKG
                     SFYWHFKDRQDLLSGVLETWKAGRIQDIVKQTRLEPGRERAQIARLVDIYSTSRSRKG
                     LLIELAVREWARRDAEAAAIVEEVDAWRLRCARELFLACGLPQHEASTRSMLLYAYVF
                     GASIMIYDRFDADIPRLTEDIRALIVGPPAPTSA"
     gene            39454..40203
                     /locus_tag="PLH85_04950"
     CDS             39454..40203
                     /locus_tag="PLH85_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNB63939.1"
                     /translation="MKILVPVKRVVDYNVKVRVKSDGSGVDVANVKMSMNPFDEIAVE
                     EAVRLKEAGVATEVVAISCGVGACQETLRTAMAIGADRGILVDTDVELQPLAVAKLLK
                     ALVDKEQPSLVVLGKQAIDDDSNQTGQMLAALLGWPQATFASKVAVGAGKATVTREID
                     GGLETLEIALPAVVTTDLRLNEPRYATLPNIMKAKKKPLETVKPADLGVDVTPRLTTL
                     KVAEPAKRSAGVVVADVGQLVDKLKNEAKVL"
     gene            40203..>40897
                     /locus_tag="PLH85_04955"
     CDS             40203..>40897
                     /locus_tag="PLH85_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB63940.1"
                     /translation="MTILVVAEHDNAALKSATLNTVTAAARLGGEVHLLVAGAGVGGV
                     AESAAKLAGLAKVLVADAAHLGEQSAENVAAQILAVVQAGGYSHVLAPATTFGKNVLP
                     RVAALLDVAQISDIVGIEASDTFVRPIYAGNALATVKSADAVKVITVRTTAFEAAGEG
                     GGAAVAAVDAVADSGQSKLVSRELTKSARPELGAAKIIVSGGRGVGSGENYHRLLEPL
                     ADKLGAALGASRAA"
BASE COUNT         6526 a        12509 c        14587 g         7075 t
ORIGIN      
        1 cgaccacgga cgcggctggc aactctgcga gcgcggcgag cagcttcgtc gagtatctgc
       61 acgcgccgac cgcgccgaca ttgacgctca gtacgccaac ggttccgact ctgtccaatg
      121 gcctgattcg ggatacctgg aacgcgtcgg tcgggcccta caacagtggc aacggcgtgg
      181 caccgaccac gatggaggcc agcatcgacg ccctgattgc ggcgggcacc gcgccgaata
      241 catcaacggt gctgacgaac gcggcggtcg cgtcggtggc ggtcggcgtc gcgtccgaga
      301 cccatggcct gatctacctg accggaggga ccgcctacac cttccagatc aagggcgatg
      361 acagcatcct gctcgagatc ggcgggcaga cggtgggcag cgtgacctgg ggaaccggca
      421 gtctggccaa tggcaagttc acgccgacca cgtccggctg gtacacgctg gatctttatc
      481 atgacaacga ggctgggccg ggtaacttca ccgtgcgcgt ctcgacgaat ggttcgtcct
      541 tcgtcaatct ggacacgacc aacttccagc tcgcccagag cgtgcaggat ctcaattcgg
      601 ccggcatccg cacgtcgagc ctgcagggtt ccagcaccac cgaacccaac gacggttact
      661 acacggtgct gccttataac gaggggcatg aggcggcttg gatcccgctg tccaccgtgg
      721 cgggtagcct ggtttccccc ggcgcgtcgg acgtcctgaa catccacatc accggccttg
      781 cgcagaacac ccaacttact gacgggaccc actgggcgac ggccgacgcg agtggcaccg
      841 tggacgtcac gggatggacg atgaatgccc tcagtgccct ggcaccggtg gactattccg
      901 gcaccatgaa tgcccaggtc caggctatcg ccaccgatac gctcaacggc gtatcaacga
      961 cgacgaacaa ggcgctcgcg atcgtggtgg atccgtatgt gacggtggcg ggcacgaccg
     1021 gcacgggtac cgacgacagg attgtcggca cgtctggcgc ggacacgctc aagggcggcg
     1081 aaggcagcga tctgatcatc ggcggagcgg gcagtgacac cctgaccggc ggcgccggtt
     1141 ccgatgtatt ccgctggatg cttgccgaca agggcacgcc tggcctgccg gcccaggaca
     1201 ccatcaccga ctggtcaagt gcggcggtga agaacggtgg cgacgtgctc gatctgcggg
     1261 acctgctcca gggcgagagc cacggggcca gcgggctggg caacctgacc agctacctgc
     1321 acttcgagaa ggtagggacg gcaacggtgg tggatatcag ttcctccggc ggctactccg
     1381 cgggcttcaa ggcgagtgcg acggatcaga ccatcgtcct gtccaacacg gatctgacca
     1441 gtggcggcac cctgaacgat gcgcagatca ttcaggatct gatgaccaag ggcaagctgc
     1501 atacggattg atcgatccgg cgaggctggc ggagtgcacg gcgccggcac aaaacaaaac
     1561 ctccaccgcc tgaggcgcgt ggaggttttg tctttggagg gcggaccaaa tgccttcacg
     1621 ttcgtccggg ccgggacgca tccggcccgg gcgacgtatc aggcgtcggc gcgggcagcg
     1681 cgcttgcgct cgtgttcctt gaggtagcgc ttgcgcaggc ggatgttctt cggcgtgatc
     1741 tccaccagct cgtcgtcggc gatgaactcg atggcggatt ccagggtgag ctggatcggc
     1801 ggcaccaggc gcacggcttc gtcggtgccg gaggcacgca cgttggtgag ctgcttgccc
     1861 ttgatggggt tcaccaccag gtcgttgtcg cgggtgtgga tgccgatgac catgccttcg
     1921 tagagggcgt cgccgggact cacgaacatg cggccgcggt cctgcagttt ccacagggcg
     1981 taggcgacgg cgtcgccgtc gtcctgagag atcagcacgc cgttgcggcg ctcactcatc
     2041 tggccggcca tggggctgta gtcgtcgaag acgtggctgg cgaggccggt gccgcgggtg
     2101 agggtgagga attcgccctg aaagccgatc aggccgcggg ccgggatgcg atattcaagg
     2161 cgcacgcggc ccttgccgtc gggctgcatg tcctgcattt cgccgcggcg gcggccgagt
     2221 tcttccatca cgccaccctg gtgttcctct tccacgtcca cgctgagcat ttcgtagggc
     2281 tcgcacttga cgccgtcgat ctccttgaag accacgcggg gccggccgac ggccagttcg
     2341 tagccttcgc ggcgcatgtt ctcgagcagg atggtgaggt gcagttcgcc gcggccggac
     2401 acttcgaagg tatcggagtc gctggtgaaa ttgacgcgca gggcgacgtt cttgaggagt
     2461 tccttctcca ggcgttcgcg gatctggcgg ctggtgacga acttgccttc acggccagcc
     2521 aggggcgagc tgttgaccat gaagttcatg gtcagggtgg gttcatccac cgcaatgggc
     2581 ggcagcggct caatggactc gggcgcgcac agggtgacgc cgatgttcac ctggtcgatg
     2641 ccggacacca gcacgatgtc gccggcttcg gccgattcgg ccggggcgcg gtcaaggccg
     2701 gtgaaggtga ggatctggct gatcttggcc ttgccccggc tttcgtcacc gtacatgacg
     2761 gccacttcct ggttggggcg gatgcggccg cgcttgatgc gaccgatgcc gagctggccg
     2821 atgtaggtgg agtagtccag ggagcagatc tggagctgca gcggcgcttc cgcatccacg
     2881 tcgggttcgg gcacgtgctg gagaatggcc tcgtagaggg gcttcatgtc ggtgccgggc
     2941 tggtcggcgt cgagcatggc gtagccgttg agggccgagg catacacgac cgggaagtcg
     3001 agctgttctt cggtggcgcc gagcttgtcg aagaggtcga aggtgtggtt gatcacccag
     3061 tccggacggg agccggggcg gtcgatcttg ttgatgacga cgatgggctt gaggccaagg
     3121 gccagcgcct tgcgggtcac gaagcgggtc tggggcatgg ggccttcgac cgcatccacc
     3181 agcagcagca cgccgtccac catggacagg acgcgctcca cttcaccgcc gaagtcggcg
     3241 tggcccgggg tgtcgacgat gttgatgtgg gtgtcgccgt actggatggc gcagttcttg
     3301 gcgaggatgg tgatgccgcg ctccttttcc aggtcgttgc tgtccatcac ccgttcggag
     3361 acctgctggt tgtcgcggaa ggtgccggac tggcgcagga gctggtcgac gagcgtggtc
     3421 ttgccgtggt ccacgtgggc gatgatggcg atgttgcgga tggagcggga catggggttg
     3481 ggttccggaa gtacttgtaa acgcgaaatt ttagcacgcc attgcgcatc gcagcatggc
     3541 cggatgcgca tctgctgcgt gctaagatgc cggccctgag cgaaattcaa ggagtgcacg
     3601 aacatgttgg ccatcatcgg cgggagtgga ctgacgcaac tggcgaacct ggaagtcctg
     3661 cgccgggagg tggtgcgcac gccctacgga gagccttcgg gcgcgctgac cttcggccgc
     3721 ctgtgtgcca atccggtggt ctttctggcg cgccatggct acggccacac gatcccgccg
     3781 caccgggtga attaccaggc caacctgtgg gcgctccagc aggctcacgt cacgtccatc
     3841 atctcggtgg cctcggtggg cggcatacgg gccgatctcg ggcccggcgc gctggtggtg
     3901 ccggaccaga tcatcgatta cacgtggggc cgcaagagca ctttctttga agggggtgac
     3961 gcgccggtgg tgcacgtgga tttcaccgag ccctacgacg ccggcgtgcg tcagcgcatc
     4021 cttgcggcgg cggtggcggc gggtgaggcg gtgtccgacg gcggagtcta tgctgcgacc
     4081 caggggccgc gtctcgagac ggcagccgag atcaaccgcc tggaacggga tggggccgac
     4141 gtggtgggca tgaccggcat gccggaggcc gtgctggcgc gggagctggg catcccctac
     4201 gcggccatca acgtggtggc caactacgcc gccgggcggg ccggcagcgc caacgggatc
     4261 tgcttcgaca gcatcgagca ggtgctgctc ggcgccatgg ggcgggtgcg ccgcgtcctc
     4321 gatcatctct gcgacgcatg atccgcgacg tcctgcgcat gggggatccg cgcctgctgg
     4381 cggtggcgcg gccggtggag gcctttgata cgcccgaact gcgaggcctg attcaggaca
     4441 tgcgcgacac catgaccgcg ctcaatggcg ccggcatcgc tgcaccgcaa atcggcgtcg
     4501 atctgagggt ggtgatcttc ggcggtggtg ggccgaatcc gcgttatccc gacgcggagt
     4561 tcgtgccgga cacggtgttg atcaacccgc agatcgagct gctgtcggac gaggaggagg
     4621 gtgcctggga aggttgcctg tcggtgccgg gcctgcgtgg ctgggtgccg cgccgggtgc
     4681 atctgcgcta tcgcggcgtc gacatggaag gtcgaccctt tgagcgggag gcgcggggat
     4741 ttcatgcccg ggtcgtgcag cacgagtgcg accacctcga cggcatcctg tatccggccc
     4801 gggtgcggga tttcagccgc ttcggcttta ccgacgtgct ctttccgggc gtgccgctgg
     4861 cggacgactg acgggccgac gggcggcccg ccggatcaga tcgccagttt ttccagcagg
     4921 tcggattcga tcttcaccac cagattgtcc tggctgaggc cggggccact gatgaggaag
     4981 gtgtcttcca cccgttcgcc gagggtggcg atcttggccg tgtgcaggtt gatgctgtag
     5041 tgggccaggg tgtcggcgat gtcgaagagc agcccggtgc ggtcggccgc ggtcaccgac
     5101 atgatgtagt gcttgccccg gtcgtccgga cggatgctga cttccggcgt gatcgggaag
     5161 tggcgcactt cccgcgacag gcggccgttg gtgggctggt cggcgggcgc ctgagtgcgc
     5221 agacgctcgg tcaggtcgtg ttccagcagg ttggtgatgt cccggtattg cagttcctgg
     5281 ccgggatcga ggaggacgaa gctgtccagc gcatagccgt ggcgggtggt gtggatcttg
     5341 gcgtcggcga tggtgaagcc gaggcgcgcg aagaagccgc acaggcgcat gaagaggtcg
     5401 cgctggtcgt gcatgtagac catgacctgg agtccttcgc ccacgtggct gaggcgtgcc
     5461 ttgaccacgg gttggtccgt ctctacgcgg tagtacagca tgcgtgcgtg ccaggcgatc
     5521 tcttctgcgt cgtgacgcag gaagtagagc gtatcgagct gggcccacaa cgcgtcctcg
     5581 gttccggggc gcaggccgta gtagcgcagc aggtgacggg cgtcgtcctg acgtttcggg
     5641 gagccgagcg cctgttgcgg cgccacgcca cgcagcaggc gctgggtggc gaagaagagg
     5701 tcctccagca gcttgccctt ccagccgttc cacaccttgg ggctggtgcc gcggatgtcc
     5761 gcatgggtga ggatgtacag ggccgtgagg tggcgttcgt cgccgacgag ggcggcgaag
     5821 cgctggatga cgctcaggtc cgaaagatcg tgtttctgcg ccacgttgga catgctcagg
     5881 tggtgctcca cgagccattc caccagaccg gtatccgtgt cgtccaggcc gtgggattcg
     5941 cagaattccc gtgcgtcctt catgcccagc ttggagtggt cgccaccgcg gcccttggcg
     6001 atgtcgtgga acagcgcggc gatgtagatc agccagggct tctcgaagct cgtggcgagg
     6061 cgcgtgagca gggggtattc gtgggcgtgg tcggccacca tgaagcgccg cacgttgcgc
     6121 agcacctgca ggatgtgctg gtcgaccgtg tagacgtgga agaggtcgtg ctgcatctgc
     6181 ccgacgatgc gccgccaggc gggcaggtag aagctgagga tgccgtactg gttcatccgc
     6241 cgcacctcgt ggagcacgcc acgcttctgc tgcaggatgc gcaggaagag cgcgcggtgg
     6301 gccggatcgc gacggaaatc cgcgttgatg agcttgcggc cgcgccacag ggcgcgcagg
     6361 gtgcgggcgg tcatgccctt gagctcggag cgctgctcca ggatcaggaa gcactcgagc
     6421 agcgcgccgg gatgccgttc gaaaacgcct tcgtcgcgca cgtcgagaag ctcgcgcacg
     6481 cactggaagc gttcgttgat gttgatggcg atggggtagc ggttgggaaa cagctctgcg
     6541 ccgaagttct gcagcaggat ggtgttgagc tgggtgacct tcttggcgct gcggtaatac
     6601 tcctgcatga acacttcgga cgcgcggcgc gtaccggtgg cttcgtagcc gaaacgtcgg
     6661 gcgagctttt cctggtggtc gaagagcagg cggtcctcgc gacggccggt cagatggtgc
     6721 agacggatgc gcacgtgctg gagaaagcgc tccacttcgc gcagttcggt ggcttcctcn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgccatggag caggcgggct
     6901 tcgaggagat tggtggtgac ggtgacgtca cgggcacttt cttcgagaca ttcctcgacg
     6961 gtgcgaacgc tgtgaccgat gtccagcccg atgtcccaga tcagaccgat cagctgttcc
     7021 agccggctgc tgagctccgg gtctgcgggg gcgtccagca ggaacagcag gtccacgtcg
     7081 gaacatggga acagctcccc gcggccatag ccgcccaccg cgacaagcgc agcgctgtcg
     7141 ggcagttgaa agatctgcca gagctcgacg agcacctcat ccaccagctg gcagcgcccc
     7201 ttgagaatcg gctcggtgag aggatgggct tcataggcgg cacgcagggt ttcggcgccg
     7261 ctcttgaggc gggtcttgat tcgggtgatg agctcgcggc tctgctcaaa gctcagcagc
     7321 gcgggtgatt cggtctggga catgtcaggg aaagaggtgg gccacgagtt ccggccggcg
     7381 cggtgcgccc tcggacaggg tgaggatgtc gacgccggtt tccgtgacca ggatcatgtg
     7441 ctcccactgg gccgacaggc tgcggtcgcg ggtgacgatg gtccagccgt caggcagctc
     7501 ggagatcgct gccttgccgg cgttgatcat cggttcgatg gtgaagatca tgcccggctt
     7561 cagttccagt ccggtacccg gcttgccgta gtggagcacc tggggatcct cgtgaaattt
     7621 ggcgccgata ccgtggccgc agaattcacg caccaccgaa aagccatggc cttcggcgtg
     7681 tttctggatc gcgtggccga tatcgccgag gtgtgcgccg ggcttgacct gggcgatgcc
     7741 cagccacagg cattcgaagg tgatctggca cagccgccgc gccaggatgc tgccggctgc
     7801 ctcgccgccg acgatgaaca tgcggctggt gtcgccgtgg tagccgttct tgatgacggt
     7861 gatgtccaga ttgacgatgt cacctttctt cagcgccttt tctcccggaa cgccgtgaca
     7921 cacctgatgg ttgatggagg tgcagatcga cttgggatag ggcttgtagc ccggcggcgc
     7981 gtaattgagc ggtgcgggga tgcatccctg cacgtccacc atgtagtcat ggcacagctt
     8041 gtcgagttcg ccggtggtga cgccgggctt cacgaaaggc gtgatgtaat cgaggacttc
     8101 ggagccgagg cggcccgcga tgcgcatctt ttcgatctct tcgggtgttt ttatggtgac
     8161 gctcatggcg gtctgcggat tcggattggg ggtgcggtag gctagctcaa gcgacgcgtc
     8221 ggggcaatcg aagcacgggc gtgcgcttcg gggcggcaat aaccgcggcg atcttgaggc
     8281 ttgggcggcc ctcctgatat actccaaagc ttgtccggca tccggtcgga caaaattcac
     8341 acgcccggtt cacaagggtc cgggatcggc gcactgcatg tgcgtggatc caggcgttcg
     8401 ccatgcatgc agatgcggca tggccggccg ggcggcggtc aaacctttgt acggagtact
     8461 tgcaatgtct gtcaccatgc gtcaaatgct cgaagcgggc gtccacttcg gccaccaaac
     8521 ccgtttctgg aacccgaaga tggccccctt catcttcggt cagcgcaaca agatccatat
     8581 cgtcaatctg gaaaagaccc tcgtcaagta caacgaggcg atggacttcg tgcgcaagct
     8641 ggccgccaat cgcggcacca tcctgttcgt cggcaccaag cgtcaggccc gcgagatcgt
     8701 cgccgaggaa gcccgtcgcg ccggcatgcc ctacgtggat gagcgctggc tgggcggcat
     8761 gctcaccaac ttcaagaccg tcaaggtctc catcaagcgc ctcaaggaca tggaagccat
     8821 ggtggaagac ggctccatcg agcgtctgtc caagaaggaa gcgctgatgg cctcccgcga
     8881 gctcgaaaag ctccagaaga gcctgggcgg catcaaggat ctgggcggtc tgcccgatgc
     8941 gctgttcgtc atcgacgtcg gctaccacaa gatcgccatc accgaagcca acaagctggg
     9001 catccccgtt gtggccgtgg tcgataccaa ccacacgccc gaaggtgtgg atttcgtgat
     9061 cccgggtaac gacgactcct cccgcgccat ccgcctctac gcccgtggcg tggccgatgc
     9121 cgtgctggaa ggcaagaacc aggtcgtgca ggaaattgtt gccgccggtt ccgatgaatt
     9181 cgtcgaagtg gaagaaggta acgaagagca ggcttaatgc cggcgttcgt gtccctcttt
     9241 caactgttcg ttaagcgtta agcattgagg agctagcatg gcggaaatta ccgcaagcat
     9301 ggtcaaggaa ctgcgcgaga agaccgacgc gcccatgatg gaatgcaaga aggcgctggc
     9361 cgaagcggcc ggcgacatgg ccaaggccga agagatcctg cgcgtcaagc tgggcaacaa
     9421 ggcctccaag gccgccgccc gcgtgaccgc cgaaggcatc gtcggtgtgt atatcggtga
     9481 aggtggcaag ctggccgcca tcgtcgaagt caattgcgaa accgacttcg tggccaagaa
     9541 cgacgacttc ctggcgctga ccaagaagct cgccgagttg gtggccgccg ccaatccggc
     9601 tgacgtggag gccctgtccg ccctgaccat tgacggccag tccgtcgagg aagtgcgcaa
     9661 ggcgctggtc ggcaagatcg gcgagaatat gagcatccgc cgttttgtgc ggaccgaagc
     9721 caagggtgcc gtggccagct acatccacgg tggtgccaag atcggcgtgc tggtggatct
     9781 ggtgggcggt gatgaagccc ttgccaagga catcgccatg catgtcgccg cggccaagcc
     9841 caaggcgctg gacgcgtccg gcgtgccgtc cgagctgctg gatgtggagc gtcgcattgc
     9901 cattgaaaag gcccgcgaag ccggcaagcc cgaagcgatg ctggaaaaga tcgccgaagg
     9961 caccgtccag aagtacctca aggacgtgac cctgctcgac caggtcttcg tcaaggccga
    10021 ggatggcaag cagaccatcg cccagctgct gaaggcgaag ggggcttccg tggccggttt
    10081 caccctcttc atcgtgggtg agggcatcga gaagaaggtg accgacttcg ccgccgaagt
    10141 ggccgcccag gccgctgctg ctgcccagaa ataagcgagg ttcctgacga tgagccagcc
    10201 cgcttacaag cgcattctcc tcaagctgtc cggcgaagcc ctgatgggtg acgacgccta
    10261 cggcatcaac caggacgtgg tgacgggcat cgtcaccgac gtggccgaag tggtccagct
    10321 cggcgtcgag gtggggatcg tgattggcgg cgggaacatc ttccgtggca tggcgggggc
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acggccgccg cgctgcgcgg
    10501 tgccgaaatc ggtgccaaca tcgtgctgaa ggcaaccaag gtggacggca tctatactgc
    10561 ggaccccaag aaggatccga cggccacccg cttccagcgc atctcctttg acgaggcgat
    10621 cagccgcaat ctggccgtca tggatgcgac cgccttcgcc ctgtgccggg accagcgtct
    10681 gcccatcaac gtgttcagcc tgttcaaggt gggcgcgctc aagcgtgtgg tgatgggtga
    10741 ggacgagggt accctggtct attgctgagg aagccgcgat gattcccgaa ctcaagaaaa
    10801 ccaccgagca caagatgctc aagtccatcg aggcgctcaa ggcggatctg gccaaggtcc
    10861 gtaccggccg cgcccacacg gggattctgg atcatgtgat ggtggagtat tacggcaatc
    10921 cggtgcccgt caaccaggtg gccaacgtga ccctcatcga cgcccgcacc attggcgtgc
    10981 agccgtggga aaagccgatg gtggggaagg tcgaaaaggc catccgtgac tccgatctgg
    11041 gtttgaatcc ggccgcccag ggtgacatca ttcgggtgcc catgcctccc ttgaccgaag
    11101 agcgtcgtaa ggagctcatc aaggtggtca agcatgaagg cgaaggtgcc aaggtggcgg
    11161 tccgcaatct gcgtcgtgat gccaacagtt cgctcaagga cgcggtcaag gccaagacca
    11221 tttccgaaga tgacgagcgt cgtgcccagg acgacatcca gaagctgacc gacaaggcca
    11281 tcgtcgaaat cgacaagctg cttgttcaaa aagaacaaga gctgatgcag atctgatccg
    11341 tcgggtcagg cgcgtcccgt ttcgtccagc ggagagatta tggtgcgatt gccttttctg
    11401 agctccactc ggggcatccc cgaggtcgta gaggttccgc gccacatcgc gatcatcatg
    11461 gatggaaacg ggcgctgggc acgcaagcgc ctgatgcccc gcgtggcggg ccatgccaag
    11521 ggtgtggagt ctttgcgggc gatgatccgc acatgccagg acctgaaggt cgagtacctc
    11581 acggtctttg cattcagttc agaaaactgg cggcgtccga aggacgaggt caccttcctc
    11641 atgaagctct tcctccgtac cctcaaggag gaaatcgagc ggatgcatcg cagcaacatt
    11701 cgtctgcggg tcgtgggcga catcagtcgc ttcgattcgg cgctcgtgaa tctcatcgag
    11761 gagtccgaga cgctgaccgc cgggaacacg gcgatgaacc tcaccatcgc ggccaattat
    11821 ggcgggcgct gggacatcct tcaggccgtc gagaagatgc tggctgcttc gcccgatgcc
    11881 cgggaagggt ttacggagga tcagcttgca gaatatctgg cgctgagctt cggtcccgaa
    11941 ccggacctct tcatccgtac cgggggcgag cagcgcatca gcaacttcat gctgtggcaa
    12001 ctggcgtaca ccgagctcta tttcaccgac aagctctggc cggaatttga cgaagcggcg
    12061 ctgaaggagg cgatccgctc ctatcagggg cgtgagcgtc gtttcgggcg aacgagcgag
    12121 cagcttgacg ccggcgctgg cgggcctgat tcaagcgatc atgcttaaaa cgcgggtcat
    12181 caccgcgttg tgcctgctgg ctggcattct ggctgcactg ttcctgctgc ccgagcccgc
    12241 ctgggtcgtg ttctgtgcgc tgatctgcgc ggcggctgcg cacgaatggg ctggcatgat
    12301 cggattcgcg gcggcccgga gggcggtcta ttccgtgctg ctcggggcgt tgtgcctggc
    12361 agggggatgt gtggccggac tgcaccggcc ggaaaccgtg gcgccctttg cactcgcgcc
    12421 ggtttatggg gtcagtgccc tgttctggct gacctgcgtg cccgtctggc tgaaatcccg
    12481 atggcaactc cgcggcgtgg gggcgggtgc cctggtgggt ctggtgctgt tgttgccccc
    12541 ctcgttggcg atcgcccatc tgcgcctgct tggcccctgg gtgctgctgg cggtgatggc
    12601 ggcagtctgg gtggccgaca tcgccgcgta tttcagcggt cgggccttcg gatcgcgcaa
    12661 gctggcgccc agcatcagtc cgggcaagag ctgggaaggg gcatacggcg cctgtgtcgg
    12721 cgtggtcgtc tatgggtttg cctgcgcggc atcggtcgga ctcgactgga ccgccccacg
    12781 ggtgtggatg cctggcgtga tcggtctgat gggcttcacg gccgtgagca tcatcggcga
    12841 cctcttcgaa tccttgatca agcgtcaggc cggcatcaag gacagcggta ccctgctgcc
    12901 cggtcacggg ggggtgcttg atcggatcga cagtctgacc tcgacgcttc ccatggcggc
    12961 cctcgtggcc ctgtggctgg ctcgctgagg gaatatccac gtgaagaagc tcattaccgt
    13021 cctcggggcg acgggttcca tcggcgtcag caccctcgat gtggtggccc gccatccgga
    13081 ccgctatggc gtattcgccc tgaccgccca tcggcaggtg gacaagctct acgaacaatg
    13141 cctcgcccat cgccccgcct acgccgtggt cgaacgggcg gaggatgcgg tggcgttgca
    13201 gcggcgcctg gacgcggcgg agtgccgttg cgacgttcta tacggcccgg ctgccctgtg
    13261 cgacgtggca tctgcgccgg acgtggcgtc cgtgatggcc gccattgtcg gcgccgcggg
    13321 tttgccgccc accctggcgg cggcgcgggc tggcaagcgg gtactgctcg ccaacaagga
    13381 ggcgctggtg atgtctggcg cactcttcat ggctgaagtg gatacccatg gcgccacgct
    13441 gctacccatc gacagcgaac acaacgccat cttccaggcg atgccgtccg gccatgcccg
    13501 gccgctcgaa accggggggg tcacgcagat tttgctcacc gcgtccggcg ggcccttccg
    13561 gtccaccccg atctcggatt tgccggctgt gacacccgac atggcctgcg ctcatcccaa
    13621 ctgggtgatg gggcgcaaga tttccgtcga ctcggccacc atgatgaaca aggggctgga
    13681 ggtcatcgaa gcgcgctggc tctttgcggc cccggcagag cgcatccagg tggtgatcca
    13741 tccccagagc gtgatccact ccctggtggc ttattccgac ggttccgtgc tggcgcaact
    13801 tggcaacccg gacatgcgca cgcccatcgc ccatgccctg gcatatcccg aacgtattga
    13861 ttcgggcgtg aagccgctgg atctgttcga agtggcccgg ctcgattttg agcggccgga
    13921 tttcgcgcgt tttccctgtc tggcgttggc ctatcgggcg ctgcaggcgg gcggcgtggc
    13981 gccggcagtg ctcaatgcgg ccaacgaggt ggcggtcgcc gcgttcctcg acggccatct
    14041 gagattcgac cgtatcgccg ccgtgattgc cgatgtgctc gacgccgtgc cggacgctgc
    14101 tgccgattcc ctggaggtgg ttttcgcggc cgatcgtgag gctcgcgaag tggcagcgtc
    14161 ccgtgtccgg gcttacctgg ctggcgcccg cccatgatcc tggtcgacta cgtcctccca
    14221 ttcctgctgg cgctggggat cctcatcgtc gtgcacgagt ttggccacta tcaggtggcg
    14281 cgctggtgcg gcgtcaaggt gttgcgtttt tccttgggtt tcggcaagcc gctgatgctg
    14341 tggcgggcgg gcgcggatcg caccgagtgg gccatcgcgg cctttccgct gggcggttac
    14401 gtgaagatgc tcgatgagcg ggaagggcag gtgccgaccg aggaactgca ccgtgccttc
    14461 aatcgccaga atgtctggaa gcgcatcgcc attgttgccg ccggaccgct ggccaatttc
    14521 ctgctcgcga tcacgctgta ctggggattg tacggaggcg gagtggagga gatccgcccg
    14581 cgggtgggca caccggtggc gggcagtcag gccgatctgg caggctttca ggacggagaa
    14641 ctggtgcgtg cggtgagcgg cgagcaggtt cagacctggc aggaattccg ctggaagctg
    14701 ctcaaccgcg ccctcgaaca ccaggctgtc gaggtcgaaa cccagaacgg acagggcgag
    14761 atcgccttcc gtcgcctcga tctcgccgga atcaccgtgg atcaggggac cgaggacatc
    14821 accgcccagc tcggcatgaa gctcttccgt ccagtgctgc ccgccgtgat cgggagcgtg
    14881 gcccccgaca gcgtggcggc ccgggcgggg ctgcaaggcg gagatcgggt gctgcgcatc
    14941 gacgacaagg atatcggcgg ctggtccgaa ctcgtcatga tggtgcgcga tgcgccgggc
    15001 cgtcgcctga gattcgaggt ggtgcggggt acggaagcgc ttgccgtgga cgtcgtgccg
    15061 gcagccgtcg aggaccgcgg tcgcacgatc ggccggatcg gcgtgggctt ggcggaaaac
    15121 gcgcagcggc gtgacgagat gtttgtgacc gtgcgctacg gaatcgtcga tgccctggga
    15181 aagggcctgt cccagacctg ggagacgacc gtgatgagcc tgtcggtgat gcgccgcatg
    15241 atcgtcggtg aggtgtcggt caagaacctg tccggacccg tgaccatcgc cgattttgcc
    15301 ggccagtcgg cgcgcatggg cttgccccac tacatcaagt tccttgccct gatcagcatc
    15361 agtctgggcg tcctgaacct cctccccatc ccgctgctcg acggggggca cttgctgtat
    15421 tatgtgctgg agatcatcaa gggcggcccc gtttccgaca ggacgatgga gatcggccag
    15481 cagataggca tggcactgct ggcgatgctc atggcatttg ccttttacaa cgacatcaac
    15541 cgccttgtag ccagctgaaa tcggatgaat tccaagcttc tccccgcggt gatcgcggcg
    15601 ctgatctgcg cctcgcccgc tctggccttt gagcccttcg tcgtcaaaga tatccgtgta
    15661 gaaggcatcc agcgcaccga agccggtacg gtcttcaact atctaccggt caaggtgggt
    15721 gagcgcttca gcgaggacaa ggcgtcccag gccatcaagg cgctgttcgc gacgggcttc
    15781 ttcaaggatg tgcgcatcga ggtggatggc gacgtgatcg tcgtcgtcgt cgacgagcgg
    15841 ccggccatcg cgcaggtgga tttcgtcggc atcaaggagt tcgacaagga ggccctgaag
    15901 aaggggttga aggaagtcgg tctcgccgag tcacggattt tcgaccgggc cgtgctcgag
    15961 cgcgccgagc aggagttgaa gcgccagtat ctgtcgaagg gcctctacgg cgtgcagatc
    16021 accacgacgg tcacgcccct cgaacgcaat cgcgtcggcg tgaatttcaa cgtggtcgaa
    16081 ggcgacgccg ccaagatccg ccagatcagc atcatcggca acaaggtgtt caaggagaag
    16141 gatctcctcg acctcttcgc cctgaccacg ccgggctgga tgacctggta ttccaagagt
    16201 gaccagtatt ccaagcagaa gctgtcgggc gatatcgagg ccctgcgcgc ctactatctg
    16261 aaccgcggct atctggattt cacggtggac tccacccagg tgtccatcac gcccgacaag
    16321 aaggatatct acatcaccct cagcatcacc gagggtgaga aatacacggt gtccgacgtc
    16381 aagctggccg gcgatctggt cttgccagag aaggaggtcc tgaacctcgt caaggtcaag
    16441 tccggcgaca cctttgtgcg tgacaacgtg acggccacca ccaaggccat cagcgatcgg
    16501 ttgggcaacg aaggatatgc gttcgccaac gtgaatgcgg cgcccgaggt ggacaaggtc
    16561 aagcgccagg tggccttcac gttctatgtc gacccggggc ggcgtgttta tgtgcgcaag
    16621 atcaatttcg ccggcaatgc ccgcacccgt gacgaggtgc tgcgccgcga gatgcgtcag
    16681 atggaaggtg cctggtacga cggggctgca ctcaaccgtt cgcggacgcg tctggatcgc
    16741 ctcggctatt tcgacgaagt caccgtcgag acgccagcgg tgccgggttc caccgaccag
    16801 gtggacgcga acttctcggt caaggagcgg gccacgggca acctgatgct gggtgcgggc
    16861 ttctccagct ctgaaaaagt catcctgtcg ggctcgatct cccagcagaa cctgttcggc
    16921 acgggtaatt ccatgaccct gcagctgaat acgggtcgca tcaaccgtac cgcggcgctg
    16981 tccttcacca atccctactg gaccatcgat ggcgtcagca tcggttggga tgtgtatcag
    17041 cgcaatgtgg atcccacgtc cctttcggtg gccagctaca agacccattc ccttggtgcc
    17101 ggactgcgct tcggttatcc gattgccgag gacgaccgca tcaactttgg tctcgcggtg
    17161 gatcgcacca agatcaccgt atttgatacc agcccgatcc agtacgtgga tttcgtcaat
    17221 aaattcggca gcactgcgga aagtctggtc agcacggtgg gctggtcgcg ggaccggcgc
    17281 gacagcttcg tctatcccac atcgggaacc tatcagcgtg tcggcctgga aatggccacc
    17341 gcgccgatgg acatccgcta tttccgctcg agttaccagc atcagcactg gattcccttc
    17401 ggcggcggct acgccctcat gctcaatggc gatgtcggct atgcccacgg ctacgacggc
    17461 aaggaactgc ccttctacaa gaacttctac gcgggcggta tcggctcggt gcgggggtat
    17521 cagcagagca cgttgggtcc gcagttcgtg gatgtatacg gcgtggcgcg cgccctcggc
    17581 ggtaaccggc ggatcatcgg caacgcggag tactacttcc cgatgccggg ccagggcaag
    17641 gaaaagtcca tgcgcatgtc gatgttcctg gacgcgggcc aagtgtgggg cgtggaagag
    17701 aaggttcgcc tgtcggattt gcgctacagt gcaggtttgg ccttttcgtg gagttccccg
    17761 gtggggccgc tcaagttcag tctgggtcag gcgctcaaga aagagcctca ggatcggacc
    17821 cagaagttcc agttccagct cggaacggtg ttttaaagag tcatcgcagg caagcggagg
    17881 tttgagtgaa aggtatcggt atttccacgc tggcgggtgt gcttctcgct gtgtttgcgc
    17941 agacggctgc tgccgaagtg aaagtgggat tcgtcaattc cgaccggatc atgaaggaag
    18001 cggttccggc caagaaagct cagcaaaagc tcgaaaagga attcgaaaag cgcgatcagg
    18061 atctgcaacg catggccaag cagctccagg gtatgcagga aggtctggaa aagaacggcg
    18121 tgaccatggc tgaaggcgaa catcgtgcca aggagcggga gttcaacgat ctgaaccggg
    18181 atttccagcg caagcagcgg gagttccgcg aggatctgaa ccagcgccgc aacgaggaac
    18241 tcgccggagt gctggagcgg gccaacaaga ccatcaaggc catcgccgag gccgagaagt
    18301 tcgacatcat cttccaggaa gcggtgtatg ccagcccgcg cattgacatc accgacaagg
    18361 tgatcaaagc gctcaccgag accaagtaag tcttggcttc gatgcagatc accctgggtg
    18421 cgctggttga gcggctgggc ggcgaactga tcggcgatcc gtccatcgtg atccgccaga
    18481 tcgcgaccct cgaaaaggcc ggggagggcg atctggcctt cctcgccaat cccaagtatc
    18541 gcgcagcact ggatggcacg acggcgtccg cggtcatcgt cgatcccaaa tccgccggac
    18601 tgaccgagcg ggcgcgcatt gtcacgcgcg atccctatct gtactttgcg cgggccgccc
    18661 agtggttcaa tccgctccgg cgtccgccgg cgggcatcca tgccagcgcg gtggtggcgt
    18721 cggcgctgcc cgaatccgtc tcggtggggc cgaatgccgt gattggcgag ggctgcgaga
    18781 tcggtgagaa cacggtcatc ggtccgggct gtcacgtcgg tgatggcgtg cggatcggcc
    18841 cggatggcct gctccacgcg ggggtcacga tttacgccga ctgcgtcgtc ggcgcccgcg
    18901 ccatcctcca ttccggcgcc gtgatcggtg ccgacggttt cggtttcgcg cgggattcag
    18961 acaaacgctg ggtgaagatt ccccagatcg gccgggtggt gatcggggac gacgtggagg
    19021 tcggcgccaa caccaccatc gaccggggcg cgctggacga cacggtgatc ggcaacgggg
    19081 tcaagctcga caacctgatc cagattgccc acaacgtcca cgtggacgac gacgccatca
    19141 tggccgggct ctccggcatc gccgggagca gccgtctcgg caaacgggtg atggtggctg
    19201 ggcaggccgg catctccggt cacctctcgg tgggagacga catcgtcgta tcggccggca
    19261 ccatcattac caagaccctc gacaagccgg gcgtctatac ggccatgctg ccgcagatga
    19321 cccacgccga ctgggtgaag aacgtctccc atgtgcggcg tctcgacggc cttgccgatc
    19381 gggtccggga actggaaaag ctgctcgctg aaaaaaacaa ggaaaattca tgagcgtcat
    19441 cgacatcaac gaaatcaagc agtatctgcc gcaccgctat cctttcctgc tggtggatcg
    19501 catcatcgag ctggaactgg acaagcgggt cgtcgggatc aagaacgtca cgatcaacga
    19561 gcccttcttt ccgggccatt ttccgaccaa tccggtcatg cccggcgtgc tgatcgtcga
    19621 agccatggcc cagaccgcgg ccgtgctggc cttcaaatca gacggctcgg tgcctgacga
    19681 gcattccacg gtgtatttcg cctcgctgga caacgtgcgc ttcaagcggc ccgtagtgcc
    19741 gggcgaccag cttgtcatgg aagccgagat cgtgcgcaag atgcgcaaca tctggaagtt
    19801 cagggcagtg gcccgcgtcg atggcgagct ggccaccgaa gccgaattca tgtgcgcgat
    19861 caagcgatga gcatccatcc taccgccatc gttcatccgg gcgcgcgcct ggccgaaggg
    19921 gtgcaggtcg gggcgtattc gatcgtcggc gagcacgtgg tcatcggtga aggcacgagc
    19981 gtcggccccc acgtggtgat cgaaggtcac acgaccattg gctgcaacaa ccgcatcttc
    20041 cagttctgct cgctgggctc cgaaccccag gacaagaagt acgccggcga accgacgcgg
    20101 cttgaaatcg gcgacggcaa caccattcgc gagttctgca gcttcagtac cggtaccgtc
    20161 caggacggcg gcctgacccg cgttggtcac gacaactgga tcatggccta tgtgcacgtg
    20221 gcccacgact gcgtcgtcgg cgatcacacc atcttcgcca acaatgccac gctggcgggc
    20281 cacgtgacgg tgggtgactg ggccattctt ggcggtttca cgggcgtgca tcagtttgtg
    20341 cgcgttggcg cccacagctt ctgtggtgtc gggacggtgc tgttgcagga tctgccgccc
    20401 ttcgtcacgg tgtccggcaa cccggcggca ccccacggca tcaacagcga aggcctgcgc
    20461 cggcgcggct acagtagtga cggcatcacg gccatcaagc gcgcctacaa gacgctctat
    20521 cggagcggtc tcaccctgga tgaggcgcgt cggcaaatcg ccgaggcagc cgccaatgtg
    20581 ccagagctgc aaatgttttc cgacttcatc gccgattccg gtcgcgggat cgtccgttaa
    20641 tccatgaccg tccgcatcgc catggtggcg ggcgaggcgt cgagcgatct gctggccagc
    20701 cacctcatcc aggcgctccg tcgtcatctg cctgatgccg agttctatgg catcggcggg
    20761 cccaagatgc aggccttggg tttccaggcc ctgtggccgg ccgaaaagct tgcggtgcat
    20821 ggctatgtgg atgccctgcg ccactaccgc gagctctccg gtatccgtcg ccagctcaag
    20881 gcgcgcatcc tggccgaccg cccggcagcc ttcatcgggg tggatgcacc ggatttcagc
    20941 ctgtggctgg agcgccgcct caaggataag ggcatcccgg cgatccactt cgtgagcccg
    21001 tccatttggg catggcggcg tggccggctg aagggcatcg cccgcgcggt gagccacatg
    21061 ctgtgtctgt ttcctttcga gcccgagctc taccgcacgg ccggagtgcc cgtgagctac
    21121 gtgggccatc cctttgccga cgtgctgcct ttggcgccgg atcgggccgg cgcccgggaa
    21181 cttctgcaac tgagtggcac ggggccggtg ttcgccctgc tgccgggcag ccgtcagtcc
    21241 gagctgcgca atctcgccga tacctacatc gccaccgcgg cgatcctgca cgagcgccat
    21301 ccagacgcca ccttcctggt gcccctggcg acccgcgaga cgcggctgct gtgggacgcg
    21361 gcgatggccc gccatgccca cctcacactg cctttgcgca ccctgttcgg tcatgccgtt
    21421 gaggccatga ctgccgccga cgtggtgctg gtggccagcg gcacggccac cctggaggcg
    21481 gccctgctca agcgcccgat ggtcatcacc taccggatcg gcaagtggtc ctggcgcctg
    21541 atgaagcgcc tggcgtatct accctgggtt ggtctgccca atgtgctggc cggtcgcgag
    21601 gtggtgccgg aactgctcca ggatgccgcc acgccccaag ccctggcgga cgccctcgat
    21661 gcctggctgc aggatgcagg ccggcgcggc gagctgctcg agaccttcac cgacatgcat
    21721 cggcaactgc gccaggacac cgcgtcgcgg gctgccgagg cgatcctgcc ttatctgcgg
    21781 ggtcgcgtct gatggagcgc ctgtgcggcg tagacgaggc cggccgcggg cccttgtgtg
    21841 gcccggtggt ggcggcggcg gtgatcctcg acccggcgcg tcccatcgcc ggcctggcgg
    21901 attccaagaa gctctctgcg gcgcgccgcg aggccctggc ccggacgatc aaggcccagg
    21961 ccctggcctg gcatatcgct gaagccagcg tcgaagagat tgaccgcctc aacatcctgc
    22021 acgcttcgat gctggccatg cagcgggccg tggcgggcct gaacctgcgg ccgacccgcg
    22081 tccaggtgga cggcaaccgc tgcccggccc tcgacctgcc ctgcgaggcg gtggtcaggg
    22141 gcgacagcct ggtggcggaa atctccgccg cgtccatcct tgccaagacg gcccgcgacg
    22201 cactgctggt ggcccttgac cggcgctatc cccagtacgg tctcgctggc cacaagggct
    22261 acccgacggc ggcgcatctg gccgccctga aggctcacgg cgcgtgcgac atctatcggc
    22321 gcagcttcgg gccggtgcgt gccgtcgtcg cccaagcttc cctgtggaac gaggatccgc
    22381 acccatgaaa gtccatcacc tgctcctgtt tctgcacctc gccggcgtga tcgtctggat
    22441 gggtggcatg gtcttcgccc acctgtgcct gcgtccggcg gccctggagc tgcctccggc
    22501 tcagcgcctg agcctgtggc gggcggtctt cgggcgtttc ttcccgctgg tggcagcggc
    22561 cgtcgccacc atcctgctga ccggctttgc catgctggcc gatgtgggct tcgcccaggc
    22621 accgcgcacc tggcacgtga tggccctgct cgggactgtg atggccggtg tcttcgcaag
    22681 tatctggttc ggtccgtggc cgcggctgcg ccaggcagtg gccgaggaaa gctgggcccg
    22741 cggcgccgag gccctggcga cgatccgtca gcgggtcggc ctcaatctgg tactgggcac
    22801 actgaccgtg gccgtggcca tcctgggcct ggccctgtag cggtcgtgaa gcgcctcacc
    22861 tcgcgggaca acgccgcggt cagacacctc cacgccctcg ccacctcggc gcgggagcgg
    22921 cgcaaggccg gcgagaccct gctcgacggg gcccatctgg tgcaggtggc cctgtcccgc
    22981 gggcagtccc tgcgccagat cgtggtcagc gacgcgggct ttgcgcaggc cgagatcgct
    23041 acgctcctgg cggcggcgcc cgacgtggcg caacttgtcc tgccggacgc cctgttcgcc
    23101 cacgtgagtc cggtggattc tcccagcggg gtgctggccg tgatggcgct cccggcggga
    23161 caggccggca acagcccgac gggtgactgc gtactgctcg atggcgtgca ggaccccggc
    23221 aacctgggca ccatcctgcg caccgcagcg gcggctggca tcgctcaggt gctgctcacc
    23281 cccggctgcg cccaggcctg gtcgccccgc gtgctgcggg cggcgatggg cgcgcatttc
    23341 ctgctgcaca tcgacgaggg catcgatgcg tcggctgcgc tggcgggcta ccccggcgca
    23401 atcctcgcca cggccctgca ggacgcgacg gagctgttcg ccaccgatct gcgcgggccg
    23461 gtggcctggc tgttcggttc ggaaggtcag ggcctgagcg aggggattgc ggcgctggcc
    23521 accggccggg tgcgcatccc catgcccggt ggcatggaat ccctcaacgt gggcgtggcg
    23581 gccggcgtat gcctgttcga gcaggtgcgc cagcgcggcc tgcatccttc cggcggctga
    23641 acgccgcccg gcgcgctcag ggcagacgca gggccgtggc agccagggcg gcgtcccggt
    23701 cttcgcggaa gggcaggacg cccagcagcg gtgccggcag ccccttttgc aaggccgcca
    23761 gattctcgtc gacacgcagc atggccgggt cgacgcggtt ggcgatccag ccggcgaagg
    23821 tcaggccgcg ggcggcgatg gcctcgacac tgagcagggc gtgattgatg cagcccaggc
    23881 gcaggcccac caccaggatc accggcaggc ccagatcgcg ggccaggtcc gcgctgtcgt
    23941 agtcgtcacc gaggggtacg cggaagccgc cgacgccttc caccaggagc aggtcggcct
    24001 gcgcgcgcag ggtgtcgaag gcggtgcgga tgcgggcggg ttcgatgcgc acgccttcct
    24061 cgcgggcagc aatgtggggc gcaatggggc tggcaaggca gtagggattg agcaacgcca
    24121 ggccgggatc gaagctgcca gcggcgcgga gacgggccgc gtcctcgttg aggaattcgc
    24181 cgttcaccca ttccgcaccg gcggcgaccg gcttcatgcc gagggcacgg agcccctggg
    24241 cacgggcggc gtggagcagg gcgcaggtgg agaaggtctt gccgatctcg gtgtcggtgc
    24301 cggtgatgaa gtaagcgctc atggtttttc ggcgacgagc cagagggtgt cataggacgc
    24361 gggcaggccg tccggggtgc ggtaggcctc gtaggcagcg gtgaggcggg ccaggtcggt
    24421 cttgcgcagg ggacgtcgcg tggcgccggt ctggtgggcg ccgagggtct tgatgtcgtg
    24481 gagcaggcgg cgcaggtccg gcgcccaggc ctgcagggtg tcccgctcgc tggcgagcag
    24541 gcgcaggccg gcggactcgg cggcgtcctc gacgacctcc ggcggctgga agcgcagcac
    24601 gtgctcggcg tcgtctacgg cggcgaaggc atcgcgcagc tcgaagaggg tgccgggccc
    24661 cagggtggcc agccacacct ggccgcccgg gcgcagcacc cgtgcgatgt cggtcagggc
    24721 ccggcgcggc gcacaccatt gcagggcgta gctggaccac agcagatcca cgcaggcatc
    24781 ggcgacgggc agctgttcca ggtccgcgca gagggggtga gcgagaccgg cgggcaggcg
    24841 cgcgagcatg gcgggggcga aatccacggc gcacagttcg gcgtcgggga agcgctgccg
    24901 caggccgggc agggcatagc cggtgccgca gccggcatcg acgacgcgag cggccgttga
    24961 aaggtggggc gtgccgaggg cgaagaggcg gtcgcaggcg ctgcgctgta cgtgggccac
    25021 cgaatcgtag gcggccgcgg cccggtcgaa ggcggcgcgg acggcgcgtt tggaggcggg
    25081 cgcgtcagtc atggcgggcg ccttccacga gggtggcgaa atcgtccggg cgtgacagga
    25141 agggcgcgtg gccgctgtcg gcgaaaatgc tcaggcgggc atggggcagg gcgtcggcca
    25201 ggtgttcggc ggcggcgacc ggcatcaggg cgtcgcgggc gccatggagg aggcgtaccg
    25261 gctgggcgat ggccggcagg atggggcgca gatcggtgtc ggccagcagg gcgaggccgt
    25321 cggcgagggc ctcgcgatgg gcggtgtcgg ccggggtgag tgcggcggtg agcgtctggg
    25381 tgacgttgcg ccgggcgtgg tcgccgaggg cctggagggc gacgaagcgg cgctgggtgg
    25441 cgtccggctc ggcggcgaag gcagtgcgga agtcgcgcag ggtgtcggcc gccagggcgc
    25501 agggccagtc gtcggtggcg gtgaagcggg ggctcgcgcc ggtgaggagc aggcgcgcaa
    25561 ccttgtcggg atgtcggcgg gcgatgtcca gggcgagcag cgcgccgagg gaccagccgg
    25621 ccagcgtgca gcctttgggc aggccggcgg cgacggcgtc ggaccaatcc gcgagccgcg
    25681 ggccggcgcg cggggcgtcg ccgtggccgg gcaggtcggg ggtgagcatc gcgtgccccg
    25741 gcagggccgc gaggaggggc gcccataccc ggtggctcag gccccagccg tggaggagga
    25801 cgagggcgct cacggggcgc cggcctccag gcgcctaagg gtggtcacga ggtggtccac
    25861 gtgttcgatc gtgtggcccg ccgacagggt gatgcgcagg cgtgccgagc ctttgggcac
    25921 ggtgggcggg cggatggcgg gcacccacag gccggcctcg tcgagggcgc gggcgacggc
    25981 gagggcttcg tggttgtcgc cgatgacgag gggttggatc ggggtcgtgg agggcatcag
    26041 atgccagcgg cgcagggtga ggccgttgcg caggcgctgg atgagggcgc tcaggtgggc
    26101 gcgccgggcg tcgccctgtt cgatgagatc gacggcggcg agcagggtgt cggcgagcag
    26161 gggcggcgcg ccggtggtga agatgtagct gcgggccttg ttgagcagcc agtcgatgac
    26221 gtcggcgcgg gcggcgacga aggcgcccgc cacgccggca gccttgccca gggtgcccat
    26281 gtagatcagg cgcggcgagc gcaggccgaa gtgggccagg ctgccgcggc cctggggccc
    26341 gagcacgcca aagccgtggg cgtcgtccac cagcagccag gctttgtggg tttcggccag
    26401 ttcgaggagc cggggcagcg gggcgatgtc gccgtccatg ctgaatacgc cgtcgctgac
    26461 gatgagcttg cgcggcgcgc tggaggcggt gagctggcgg gcgagggcgt ccacgtcgcc
    26521 gtgggggtag cggtgcagat cggcgcggct gaggagggcg ccgtccacca gggaggcgtg
    26581 gttgaggcgg tcggcgaaga cggcatcgcc gcggccgacg agggcgggca tgacgccgat
    26641 gttggccatg tagccggtgg tgaagcagag ggcggcgtcg aagccggtga aggcggcgag
    26701 gcgggcttcg agggcatcgt gggcgaccgt gtggccgctc accagatggg cggctccgga
    26761 gcccacgccc cagcgggcgg cgccggcgcg ggcggcgtcg atgagggccg ggtcggacgc
    26821 gaggcccagg tagtcgttgg agcagaaggc aatgcgcgcg ctgccgtcgg ccagggtggc
    26881 ggccggcgcg caggggctgg cgacgacgcg gcggcggcgg cgcaggccgg cggcgtccag
    26941 ctctgcaagg ccggtggcaa gggcgtcgag ggggtcgtgg gcctcagccg gcaagtgcgg
    27001 cctccagggc ttcggccgag cggcgggcga ggaagtcgat gtgcacgtcg tcgaggatgt
    27061 agggcggcag ggtgtagacg gtgggaccga tggggcgcag caggagttcg cgctggaggg
    27121 cttcggcaaa gtagcggcgg gcgaagtcgg gcggggcgtc ggccacgtcg aatgcgagga
    27181 tcatgccctg ctggcgcaga tgccggaccc gcggatggtc gccgacagcg gcgcggaagg
    27241 cctcgccgag gcgggtggcg cggtggcggt tggcggcgat cacctcgtcc tcggcgaaga
    27301 tgtccagggt cgccagggcg gcccggcagg cgaggggatt gccggtgtag gagtgcgagt
    27361 gcaggaagcc gcgggcggtg tcgttgtcgt agaagacgtt gtagacggcg tcgcgggtga
    27421 ggacgatgga caggggcagg tagccgccgg agatgccttt ggacaggcac aggaaatccg
    27481 gtgtgatgcc ggcctgctcg caggcgaaga agctgccgct gcggccgcag ccgacggcga
    27541 tctcgtcggc gatgaggtgt acctggtggc ggtcgcacag ggcgcgggcc agacgcaggt
    27601 attccgggtc gtacatggcc atgcccgagg cgcactggac gaggggttcg acgatcaacg
    27661 cggcggtctc gccggcatgt tcggcgagga aggtttccag tgcggcggcg gcgcggcggg
    27721 ccacatctac ggcagtttcg ccgggcatcg cctggcgtgc gtccgggttg ggtacgcagc
    27781 ttgccgggcg gatcaagggg gcgtaggcat cgcggaacag ggccacgtcg gtgacagcga
    27841 gggctccgac ggtttcgccg tggtagctgc cggcgaggca gacgaagcgg ttcttgtggg
    27901 gctgcccctg attgcgccag aagtgggcgc tcatcttgag ggcgatctcg gtggcggagg
    27961 cgccgtcgga ggcgtagaag gcgtggccga ggcggtggcc ggtgagggcg gcaaggcgct
    28021 cggagagctc gacgaccggt tcgtgggtga agcccgccag caggacgtgt tcgaggcggt
    28081 cgagctggtc cttgatggca tggccgatgc gcgggttcgc atggccgaag aggttgaccc
    28141 accaggagct gatgccgtcc aggtagcgcc ggccgtccat gtcgtagagc cagggcccct
    28201 caccccgcgc gatggggatc agcgggagct gctcgtggtg cttcatctgg gtgcaggggt
    28261 gccagacggc ggcgagagag cgggcgagga gggcggcgtt ggacatgggg cggggcgctg
    28321 caggttcgat ttccgggtcc gaaaggaccg agtggagcgg ccttgcgggc gctccactcg
    28381 gagaccgggc cggcggggct tactgggagt aggccatggc cttgttggcg cccaccgcga
    28441 tgtactggtt ggcggccttc atgagggtgt agaggtcgtt gccgctgctg gcagcgtagt
    28501 aggtccaggt cacgccgtcg gggctggtga aggccgcgcc gttggcgccc acggcgacga
    28561 actggttgct gccgatgacg gaatacaggg tgaccgccgg cgagacggtg gacgcggtcc
    28621 aggtggtgcc gtcgctgctg gtgaggacct gaccgcccgc gcccgtggcc acgtaggtat
    28681 aggtggtggt gctgccggag gtggtggcca tgccggacac tccgtagagg gtggcggcgg
    28741 tgccggaggt gcgttgggtc cagttggtgc catccgagct ggtcagcacc acaccgccgg
    28801 cgcctacggc gatccacagg ccggacgggg tgtagctgat gccgtacagg tggccgatgc
    28861 cggacggtac gctggccgca gcggtccagg tgccggcgct ggtggaatag cggatcgtgc
    28921 cgttctcgcc gaccgccact gcgacaccgc cggaggtggc gacggcggtg agcttgttgg
    28981 tggtgttgga ggtggcctgg gtccacgtgg acaggtcggt gctgtagtag atggcgccac
    29041 cgtcgccgac cgcgatgaag gtagacaggc cgacggtcat ggcattgacg ttggcggtgc
    29101 cggcgggatt ggcgagggct gtccaggtgt gaccatcggt ggacgagaag gcggcaccag
    29161 ctgtgcctgc cgccacgtag gtggcggtgg tgaccgtgga ggtggtgccg taggcgagac
    29221 cgcgcatgtc ggcggtaagg ttggtgacgg ccgtccaggt ggtgccggcg agacgtgggg
    29281 tggtcgtccc gtatgcgtag tcgccgccct tgccaccgtt gatccggccg ttgagcgcga
    29341 aggcgtactg ggtgccgttg gtcaggccgg tgacgacgta gggcgagccg atgttgatgt
    29401 gatagcggat gcccggcgtg ttggagtcgc tcggattgat ggtgctgccc ggcgcatacc
    29461 agagccagta ttcgactccg gcgtcctgtt gccaggtgat ggtgacctgg gtgtcgccgg
    29521 gggtcacggt gatgccgccc gtcggtgcat tggctgaact gcccttgcca ccgcagccgg
    29581 cgaggatgac ggaaccgagc agggcgccga cgacgaggcg gcgccgggac aggaaggcgg
    29641 gacgcaggga catggggttg gccaggactg ttcgtgggaa gtcccgcatt ctacatcacg
    29701 gccgggccga tcccgcctgt ccggccgtaa gcggatatgt ccggcgcggc cgcgtcctca
    29761 cgggagggtc acgccggcca cgtccttgag gagcagttcc tgggcggcgc gccggctggt
    29821 gcggggattc ttgcgccggt agtggttgtc ctggtccagc tcccaggcct ggcagttgtc
    29881 gcccaggtag gggcgcaggc cctccttgac gacgcgtcgc ttgaccctgg ggtcgaggat
    29941 ggggaaagcc acctcgatgc gcttgaagaa gttgcgctcc atccagtcgg cactggagag
    30001 gtagagcttc tcggcgccgt cggcgtggaa gtagaagata cggtggtgtt cgaggaagcg
    30061 gcccaccacc gagcggaccc ggatgttctc ggagaggccg ggcacgccgg gcttgagggc
    30121 acacacgctg cgggcgatca ctgtgatgtc cacgccggcg caggaggcgt cgtagagggc
    30181 ctcgatggtt tccgcttcga gcagggcatt gaccttgacc acgatgcggg ccttgcggcc
    30241 ggcgcgggcg atttcggctt cgttgcggat ggcggcgacc acgttgggct gcagcgtaaa
    30301 aggcgcctgc cacaggtggc gcagcttgcc cgcctggccc aggccggtaa gctgcttgaa
    30361 gacctcggcc acgtcggcgc cgatctcctc gttggcggtg aggaggccga agtcggtgta
    30421 gaagcgggtc gtgcgtgggt ggtagttgcc ggtgcccagg tgcacgtagc gcttcagccc
    30481 gcggtcctca cgacgcacca gcaggagcat cttggcgtgg gtcttgtagc cgaacacgcc
    30541 atagaccacg tgggcgccgg cctcttccag gcgggcggcc cagctgatgt tggcctcttc
    30601 gtcgaagcgg gccatcagtt cgagcaccac ggtgacttcc ttgcccttct gggcggcgcg
    30661 gacgaggtgt tccatcagca cggagtcggt gccggtgcgg tacacggtca tcttgacggc
    30721 catcacctgc ggatcgtcgg ccgcggcctg gaggagctcg atgaccggct cgaagctctg
    30781 gaagggatga tgcagcagga tgtcctggcg gcggatggcg gcgaagatgt tgtcgcgctt
    30841 gtcgaggccc ttgggacgcg ccggcaggaa ggggcggtat ttcaggtccg ggcggtccac
    30901 ccagtccggc acctggatca ggcgcaccgg attgacgatg cccggcacgc gatacacgtc
    30961 cgcgtcgcca aggccgaact ggctcttgag gaagtcggcc atggcgctgg agcagttgtc
    31021 ggccacttcc aggcgcacgc cgtcgccgaa gtggcgctgc tggagttcgc ccttgagggt
    31081 ggcgcgcagg tccttcacct cttcctcgtc cacgtagaga tcggagttgc gggtgacgcg
    31141 gaactggtac acgccctgca cgttcatgcc ttcgaacagg gtgcccacgt gggcgtggag
    31201 cagggacgac aggaagacgt aggtgtattc ggcgtcggcc agctcccgcg gcacgcggat
    31261 gacccgcggc agggcgcggg gcgcctggac gatggcggcg ccggagttgc ggccgaaggc
    31321 gtcacggcct tccagttcca cggcgaaatt gaggctcttg ttgagcaccc gcgggaaagg
    31381 atgggccgga tcgaggccga tgggggtgag gacgggcatc acctcgcgca ggaagtagtc
    31441 ccgcgcccag gcgtgctggg cctcggtcca cgccacccgg cgcaggaata cgatgccttc
    31501 ggcggcaagg gccggcagga tggtgtcgtt gagcagcgcg tactgctcgc tgatgatgct
    31561 gtgcacctcc cggctgatgt gctcgtagag cacccgcggc gaggtgccgt cggccgtggt
    31621 ggcgacccgc ccgaggcgca tctgctcctt cacgccggcc acgcggatct cgaagaattc
    31681 gtccaggttg ctggaaacga tgcacaggaa gcgcagccgc tccagcagcg gcacggcggg
    31741 atccgccgcc tgggcgagca cccggcgctg gaactggagc agggacagct cgcgattgat
    31801 gaagtgatcg gtgctgtagc ggggtgcggg ggcggggcgc atgggcggat ccttgttctg
    31861 ttgtctctcc ggacgaggat tctgtgacag ttttattgcg atcgtgtgac ggcggcgaag
    31921 aagggcggtg cggacggtgg ggtgctagaa tgcagcccct gcgtgccttc cggcgcacct
    31981 tgcgaccgat tcatgaacca cgaactgatc gccgccatcg acctgggttc caacagcttc
    32041 cggctccagg tcggacggat tgtaaacgac caaatctatc ctctcgacgg catcaaggag
    32101 tcggtccgcc tcgcggccgg tctgtcgccc gaccgcatgc tggacgccgc cgcccagttc
    32161 cgcggcctca acgcattgcg ctgcttcaac gagcgcatcc gggattatcc gccggaggcg
    32221 gtgcgcgcgg tggccaccaa caccctgcgg gtggccaaga acgcgccaca cttcctcatc
    32281 caggccgaag cggcgctggg atttcccatc gaagtgatcg ccggtcgcga ggaagcccgc
    32341 ctgatctacg tgggcgtcgc ccataccctg cccaatccgc accgccagca actggtggtg
    32401 gatatcggcg gcggctccac cgagttcatc atcggcaaga gcttcaagcc gctggaactg
    32461 gagtccctgt acatgggctg cgtgggttat tcgctgcgtt acttcccgga cgggcgggtg
    32521 gacaagaaag ggttgcggga agccgagctg gccgcgctca aggaaatcca gaccatcagc
    32581 cacgcctacc gccgcaccgg ctgggaagag gcggtcgggt cgagcggatc ggccaaggcg
    32641 ctggtcgaga tcctcgagct caacggcctg tcggctggcg gcatcacccg cgacgggctg
    32701 gagaagctca aggtgctgat gctcaaggcc ggcagcgtcc aggccctgca gctggaaggc
    32761 ctgcggccgg atcgactgcc ggtgctgccc ggcggctttg ccatcatgtc ggcgatcttc
    32821 caggagttcg gcctggagcg catggtgttt tcggaaggtg ccctgcgtct cggagtgctc
    32881 tacgacctgc tcggccgcta ccaccaccac gatctgcgcg acgccaccgt cacggccttc
    32941 atggagcgtt acagcgtgga tgtcgcccac gcccgccggg tcgccgatac cgcgctgcat
    33001 ttcctggagc agctcgatcc atcgacggaa gatcccgaga acacggatcg ccgcttcgtg
    33061 gaatgggctg cgctgctcca cgaggtgggc gtgtcggtgg cccattccag ctaccacaaa
    33121 cacagtgcct acatcctcgg caacgccgac atgccgggct tctcgcggat ggaccaggga
    33181 cgcctgtcgc gcatggtgct ggcccaccgg ggcaagctcg cgcgggtcag cgcactggat
    33241 ccggaaagcc tggagtggcg cttcatcctg tgcctgcgtc tggcggcggt ggtgcatcgc
    33301 gcccgcgacg gccgcggtgt gccgcccttg cgggtgaagc gcgaaggccg cgcctacgtg
    33361 ctcctggcgg acggtgactg gctgcgcaac ctgccgctca cggcggcggc cctggacgag
    33421 gaagagcgcc agtggcaggg catcggcatg ccgctgcggg tgcgcgtcga acgggcggcg
    33481 cgcggggcgg cctgaggcgt cacatggcgt gcggtagccc atgaatgcgg ccccgcaggc
    33541 agcgccggcg ctccgggtgg acggtcacgg cgccgcggcg acggtgaccc tgacgggcga
    33601 ctggaccctg cgggcattgc tgccgcgcct gtcggtggtg caggcggccc tcgccgaggt
    33661 gcacggggtg gccgtctggg atctggacgg ggtcatccgt ctcgacacct ttggcgcgac
    33721 cctgctgtgg cgccactggg gccggcaacg accggcccgt ctgcaggtgc cgaccatcct
    33781 cgacgaaaac tttgtccgtc ttgcggcgct gggggggccg tcggtgccgg tcgcgccgcc
    33841 ttaccgcttt ctcgacgcga cggcgcatct gggccgtctg gcgattggct tcgggcggca
    33901 tctggcggat ttcaccgccc tggtcgggca gatcctgctg gacgccctcc atctgctggg
    33961 ggatcgccag cgctggccgt ggcgcgagat gtcggccaat ttctacaagg tgggcgtcaa
    34021 ggccatgccg gtgacggcgc tggtgggctt cctgatcggc gtcgtgctgt cgtatctgtc
    34081 ggcgctacag ctgcgggcct tcggtgcgga catctacatc gtcaacatcc ttggcctggg
    34141 catcatccgc gagctgggcc cggtgctggt ggcggtattg gtggccgggc gctcgggttc
    34201 ggcgatgacc gcccagctcg gcgtgatgcg ggtcaccgaa gagatcgacg cgctggcgac
    34261 catggggatc tcgcgttacg tgcgactggt gctacccaag gtggtggccc tgttcctggc
    34321 gatgccgctg ctcaccctgt ggtgctcggc ggtggcgatc gtgggcggca tggtgtcggc
    34381 ctggctccag ctggggatcg gcttcgagta cttcctgcag gcgctgccgc gggccgtgcc
    34441 ctttcccaat ctggtgatcg ggttgtccaa gggcgcggcg ttcggtgccc tgatcgctct
    34501 ggtggcctgt catttcggct tgcgggtcaa gcccaatacg gagagtctgg cggcccatac
    34561 cacggcatcg gtggtgaccg cgatcaccct ggtgatcctg acggacgccg tatttgccat
    34621 cgccacccgc tccatcgggg tgccaggatg agcgccttcc cggtagaggt gaaggggctg
    34681 tccacccgtt ttggtgacac ctgggtgcat cgcggcatcg acctggcggt gccagcgggt
    34741 gagatcgtcg ccctggtggg tggctcgggc agtggcaaga ccaccctgct gcgtcagatc
    34801 gtcggcctgc tgcgtcctac cgcgggggag gtgcgcctgt tcggcgaacc cctgttcggt
    34861 ggcagccgcc tcgagcaggt ggcccggcgg cggcgcttcg gcatgctgtt ccagcagggc
    34921 gcgctgttct cggccctcga cgtctacgcc aacattgcct ttccgttgca ggaagcaggt
    34981 gttcgggacg aggcgatgct ccacgacctg gtgggcgtca agctggccat ggtggagctg
    35041 gcgccggagg tgggtctgcg catgcctgcg gagctgtccg gcggcatggt caagcgggtg
    35101 gccctggcgc gggcgctggc cctggagccc gagctgctcc tcctcgacga acccaccgcc
    35161 gggctcgacc cggaccgtag cgcgggcttc gtgcgtctga tccgcgacct gcaccgggcg
    35221 ctgggcctga cggtggtgct ggtgacccac gatctggata ccctggccgc cctggcgacc
    35281 cgcgtcgccg tgctggcgga aggacgtata ctgggcttcg gttccctgga ttccgtcatg
    35341 gcactggagc atcccttcat cgaacgtttc ttccgaggcg agcgcagtcg tgcggcgctg
    35401 gcgcgggggc gggcggaggc ctgatggaag atcgcgccca cgccatcgcc gccggcctgt
    35461 tcgcgctgct gctgggtacg gcgctggtgt tcaccctgtg gtggttctcg gacaagcggg
    35521 aggtgacccg cgacgtggtg ctggtcagcg ccggcagcgt gaatggcctc aatccccagg
    35581 ccacggtacg ctaccggggc atcatggcag gcaaggtggc gagcatcgcc ctcgacccgg
    35641 cgcggccgca ggatctgctg gtcaccgcgc gcatccgtgc cgatctgccc ctgacccacg
    35701 gcacgcgggc gcgtctggcg acccagggcg tcaccggcat cgccttcatt gccctcgacg
    35761 acagcggcgc cgatccgaca cccctcgcgg ccgacggcaa cgggccgccc cgcattgccc
    35821 tggagccagg cctggtggaa gaggtggccg atgcgacgcg gcgcaccttg ggccagctgc
    35881 gcgagctgtc cgaacgcttt gcgcggctcg ccagcaacga gaacatcggc cgcgtggagc
    35941 gcatcctggc gcatatggag tcgtcttcca aaggtctgga caagggcatg caggatgccg
    36001 cggcgtccct ggcagcggtg catcggatgc tcggcaagga caacctggcg cgcatcgaac
    36061 gtacgttggc caatgccgaa cgcttcagca gcgatgccgc gccgctggcc gccgacggtc
    36121 gccgtctgct ggcgcgcatg caggatgtgg ccgaccgtct tgacgtgatc gccgcctcca
    36181 ccggcgaagg cttcgccacc agcaccctgc cgcgcctcaa cgtgctgctg caggaactga
    36241 ccaccacctc gcggcagatg aacgatctgc tcgacgagct ggattcccat ccgcagatct
    36301 tcctgctcgg gcgcggcaag gccccgccgg ggcccgggga ggcgggattt gtcccgaatc
    36361 gaccatgagc catccatgac gcattccctg cgtgcctgcc tgtcggcgtc tgtgtcgctc
    36421 gtcgtggccc ttgtaagcct ggcgggctgt ggcgccgtgc tgccgaaggg cgccccgacc
    36481 ttcgccgtgc atgattttgg cccgctggcg ccggtcgggc cccgcagtct gccctacccg
    36541 atccgcacgc tggaggtgat ccccgcgccc tggctggcgt ccacggccat gcagtaccga
    36601 ctggcctacg cccaggagac gcgccgtcag gccttcgtgc agagccgctg ggcggcgcag
    36661 ccgggtcagt tgctggaggt ggccctgaag cgttcggtgc gggttaacgt gaccaccgtg
    36721 agtctcggcg gctgccgtct gcgggtcgat ctggacgagt tcatgcaggt cttcgacaat
    36781 gagtcaggca gccggggtgt catcgacgcg cgggcgtccc tgctcgcgcc gcggaccgac
    36841 cagctcctgg ccacccaggg cttcagcgtg acccgtccgg cgtcgagccc ggatgcggcc
    36901 ggaggcgtcg tggcgctgcg ggacgccgtc caggcgttcg acgcggccct gttgacctgg
    36961 ctcgacggtc tggcgggcag cgtcggagcg cgatgcagcc agtaaatccg ggcgaaccga
    37021 tttttttcac cggccatttc gagccggcat tttcaaagta cggaggagaa ctcatgagcg
    37081 cagtcgtcga caccccccag gaacccatgc tgctgcgcac cgacgcggac ggcgtgacca
    37141 ccctgaccct gaaccgtccg ggccagttca atgccctgtc ccaggccatg ctcgaagcct
    37201 tgctggcgga gctggagcgc atcgccgagg acagcgcggt gcgcgtcgtg gtgctggccg
    37261 gcgccggcaa ggccttctgc gccggccacg acctgaagga aatgcgcggc aattacacca
    37321 agtccttcca gcaggccctg ttccgcctgt gcggaaaggt gatgatgaag atcgtcgcca
    37381 tgccgcagcc ggtggtcgcg cgcatccacg gcatcgctac ggcggcgggc tgccagctgg
    37441 tgtccatgtg cgatctggcg gtggcctcgg acgtggcgcg ctttgccgtg tccgggatca
    37501 acgtggggct cttctgcgcc acgcccagcg tgggcctggc ccgcaacatg gggcgcaagg
    37561 aagccttcga gatgctggtc accggcgatt tcatcgacgc ccaggaagcc cgccgccgtg
    37621 gtctggtgaa tcgcgtggtg cctttggagc aactcgacga cgaggtcgcg aagctgaccg
    37681 cctccatctg cgccaagtcg cccgccgcca tcgcgatggg caagcgcatg ttctatgcgc
    37741 agctggagat ggggcttgac gccgcctacc agatggccgc cgaggccatg gcctgcaaca
    37801 tgatgtgcga ggacgcggcc gagggtatcg acgccttcat ggccaagcgc acgccggtgt
    37861 ggaccggccg ctgaggcggc ccgcgtgctg atcgacaccc actgccatct ggacgccgcg
    37921 gaattcgccg acgatcggtc ggcgcagatc gacgcggcgc gggcggcggg tgtcggccgc
    37981 ttcctggtgc cggcggtgga cgcgggcaac ctggacgccg tggccgcgct gtcggcggcc
    38041 cacgcggact gccgccacgc cttcggcatt catccgatgt acgtggcgcg ggcgcaggcg
    38101 gacgatctcg agcgtatacg gtcgcggctc gccgacggct ccgcggtggc ggtgggggag
    38161 atcggcctcg atgcttacgt gccggacggt gacttgccgc ggcagacgga attcttcgag
    38221 gcgcagcttc gcctggcgcg gacgttcgac ctgccggtgg tcctgcacat ccgccgggcc
    38281 caggacgcag tgttgggcgc cctgcggcgg atcagggtgc ggggcggcat cgctcatgcc
    38341 tttaatggaa gccgccagca ggccgaggcc ttcatcgggc tgggcttcaa gctggggttt
    38401 ggcggtgcga tgacctattc gggttcgcgg cgcatccgcg aactggccgc aacgctgccc
    38461 ctcgacgcgc tggtgctgga aaccgatgcg ccggacattc cgccggcgtg ggccgcccgg
    38521 cagcgtaacc tgccggccaa tctgcgccgt attgccgccg agctggcggc gctgcggggc
    38581 atggacgagg cggagctgat cgccgcgacg gggcgcaacg ccgtggccgt gattgccggc
    38641 ctgcgttgat gccgtgcatc acgccgacgt cggcgccggc gggccgacga tcagcgcgcg
    38701 gatgtcttcc gtgaggcgcg ggatgtccgc gtcgaagcgg tcgtagatca tgatggacgc
    38761 gccgaaaacg taggcataga gcagcatgct gcgggtcgag gcctcgtgct ggggcaggcc
    38821 acaggccagg aacagctcgc gggcgcagcg caggcgccag gcgtccacct cttcaacgat
    38881 ggcggcggcc tcggcgtcgc ggcgcgccca ttcccgcaca gccagttcga tgagcaggcc
    38941 cttgcggctg cggctggtgc tgtagatgtc caccaggcgg gcgatctggg cccgctcccg
    39001 gcccggttcg aggcgcgtct gcttgacgat gtcctggatg cggccggctt tccaggtctc
    39061 cagcacgccg gacagcagat cctggcgatc cttgaaatgc cagtagaagc tgcccttggt
    39121 cacgcgcaga cgcttggcga ggacttccac ccgcatgccc tgcacgcctt cgtcggcgag
    39181 gacttccagg gcggcctgga tccaggcggc gcggtcgagc tgggggcggg tcgttttttc
    39241 catacggcgt agtattgaca ggtttgtcgt ccatacgcta ccgtatggaa agtgacaaag
    39301 caagcgggcg tgtgagaagc tgccggtttg cggttcaggc gccgtcccga gcgtcggcgc
    39361 atccagaaga acggccgtgc aggcaggcgg agttttgcct gcggcggtcg cataagcgtt
    39421 ggtggagaac ctttttgagg aaggagcaca gcattgaaga ttctggttcc cgttaagcga
    39481 gtggtcgatt acaacgtcaa ggtccgggtc aagagcgacg ggtccggcgt ggatgtggcc
    39541 aacgtcaaga tgtccatgaa ccccttcgac gaaattgcgg tcgaagaagc cgtgcgtctc
    39601 aaggaagccg gcgtggcgac cgaggtcgtg gcgatctcct gcggtgtcgg cgcctgccag
    39661 gagaccctgc gcaccgccat ggcgatcggt gccgaccggg gcatcctcgt cgacaccgac
    39721 gtggagcttc agcccctggc cgtggccaag ctgctcaagg ccctggtgga caaggagcag
    39781 cccagcctgg tggtgctcgg caagcaggcc atcgacgacg attccaatca gaccgggcag
    39841 atgcttgccg cgctcctcgg ctggccccag gccaccttcg cctccaaggt ggcggtgggc
    39901 gcgggcaagg cgaccgtgac gcgggagatc gacggtggtc tggaaaccct cgagatcgcg
    39961 ctgccggcgg tggtgaccac cgacctgcgc ctgaacgagc cgcgctatgc cacgctgccc
    40021 aacatcatga aggccaagaa gaagccgctg gaaaccgtca aacccgccga tctgggcgtg
    40081 gacgtgacgc cgcgtctgac caccctcaag gtggccgagc cggccaagcg cagcgccggc
    40141 gtggtggtgg ctgatgtcgg tcagctggtg gacaaactga agaacgaagc gaaggtgctg
    40201 tgatgacgat tctggttgtt gcggaacatg acaatgccgc gctgaagagc gcgaccctca
    40261 acaccgtcac cgcggcggcc cgtctgggcg gcgaggtgca cctgctggtg gcgggcgcgg
    40321 gcgtcggcgg tgtggctgaa agtgccgcga agctggctgg cttggccaag gtgctcgtcg
    40381 ccgatgcagc ccacctgggc gaacagtcgg cagagaacgt ggcggcccag atcctcgcgg
    40441 tggtccaggc cggtggttac agccacgtgc tcgcgccggc caccaccttc ggcaagaacg
    40501 tcctgccccg cgtcgcggca ctgctggacg tggcgcagat ttccgacatc gtcggcatcg
    40561 aggcatcgga caccttcgta cgtccgatct atgcgggcaa cgccctggcg accgtgaaga
    40621 gtgcggacgc cgtcaaggtc atcaccgtgc gcacaacggc cttcgaggcg gcgggcgagg
    40681 gtggcggcgc ggcggtggct gcagtggatg ccgtcgcgga cagtggccag agcaagctgg
    40741 tcagtcgtga gctcaccaag tcggcccggc ctgagctggg tgcggccaag atcatcgttt
    40801 ccggcgggcg cggggtgggc agcggcgaaa actaccatcg tctgctcgaa cccctggccg
    40861 acaagctcgg cgccgccctg ggtgcgtccc gcgcggc
//
LOCUS       DAOQMM010000018        40569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_235_length_40569_cov_49.284988, whole genome shotgun sequence.
ACCESSION   DAOQMM010000018 DAOQMM010000000
VERSION     DAOQMM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 40569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40569
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235_length_40569_cov_49.284988"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1153)
                     /locus_tag="PLH85_04960"
     CDS             complement(5..1153)
                     /locus_tag="PLH85_04960"
                     /inference="COORDINATES: protein motif:HMM:NF018809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalM family protein"
                     /protein_id="HNB63941.1"
                     /translation="MLNNFGRSSRWFCFALVWSVLLSGCANVAFDHGVSRLDGKALVF
                     GRIVLDREGERLVISPFSMPVVIRDISSADEPRMLAQKFENDGRFYWALPPGRYQVSI
                     VLHNYAGGVVSYSFALERSGRSYYFGDLILHGKKRFDSVGSANMRDIKPEFVNDLQGA
                     KAELLRRNPQLDAASIDRLTVRDMTSPEQRGVAYGEVLSELRPCCASLASLPYKKLSS
                     GQRGEDTISSNSPVFDFPEGRSRFVAWELSAPSMQNTVALRSVVTPSGLPGTGQFYIF
                     SPAVMLLDENFNVLADLQRGLFVPVPASMMPPRSASLQARIPLNAQTARVRYLVVYTT
                     RSIIEGAWSTSRPGFVPIAGGVLPTGIPIGVVMEPAISGIIEAELSRH"
     gene            1437..2153
                     /locus_tag="PLH85_04965"
     CDS             1437..2153
                     /locus_tag="PLH85_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB63942.1"
                     /translation="MKLDRLLQAQGFGSRRQCRQLVELGEVRVRGDVVDDPDAAIDTA
                     GLTYEVAGEAWIYREKTYLALFKPAGFECSHEPKHHRSVYSLLPHPLVVRNVQSVGRL
                     DVDTTGLLLFSDDGAFIHTLSSPKKHVPKIYRAELAEPAADTLVPRLLAGVDLIDEPR
                     PVAALDACLRGPTTLEMRLDLGKYHVVKRMIAAAGNHVQTLHRSGFGGLSLGEGPLAG
                     LAEGQFIYLDADALRQLRAV"
     gene            2164..3903
                     /locus_tag="PLH85_04970"
     CDS             2164..3903
                     /locus_tag="PLH85_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB63943.1"
                     /translation="MSDTLRRRPMLPTRTGTLRLKSGTPTPAGDRPATQDWRPPAARR
                     GKASMPEQANTESNRRPPREGDVRGDVPPRRFGDERPPRTDERRPGRNADERFRRDDN
                     GHPPRADFRKRRDDTDRDPRRFDDDRRPARSDDTGVRRDRDTRPARPFRLPDERRREA
                     EDGRPPRGGFRDRRDDAPDRAPRRFEDERPPRNDDGRPGRAADERFRRDDDRRPPRGD
                     FRERPRREDTDRGPRRFADERPARGPQEARPPRSDAPRRPGREDDRRPPRDRDDRPAR
                     GADARGRRDEARPFSGGNPDAPAPAGRPRPVARPDAPPRHERKPWNGEVRPAPAPSPL
                     RIDGSIRLSKRMTELGLCSRREADEYIEKGWVRVDGRVVSELGARVKPGQTIELDRRG
                     QTAQERRVTILLNKPVGYVSGQAEDGYEPASVLLTPENRWAEDPNETRWLRTHLKGLA
                     PAGRLDIDSVGLLVFTQDGRIARALIGDDSDVEKEYHVRVEGRLPPEGLALLNHGLSL
                     DGQALRPAKVSWLNEDQLRFVLREGKKRQIRRMCELVGLKVVGLKRVRIGRVLLGPLP
                     PGQWRYLAAGEKF"
     gene            complement(3923..4831)
                     /locus_tag="PLH85_04975"
     CDS             complement(3923..4831)
                     /locus_tag="PLH85_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNB63944.1"
                     /translation="MKAWNGWCSAARWAVVALAMVWSGLAAARGPLFLWEVLDDAGTV
                     RAYLYGTIHVCDAACFPLPARVTEAFARSDSLALELDPEDPALAGVLARAGVLPEDQR
                     LDDRLPAALRPRLTAAARLLGIPPAVLQRLQPWMASTVLTLRAAAQAGFVTEQGIDLW
                     LARAAQARGLPLRPLETVERQLAALSAGGDAAQAAGVEEVVDLVENEAGREYFGALVQ
                     AWRAGDVGELDHLMREVTSSPAMAPLLEDMLDARNREMAETLVGRLTPGARPFVAVGA
                     GHFGQDSGLLAQLARRGFRLRQVEAD"
     gene            4871..5836
                     /gene="miaA"
                     /locus_tag="PLH85_04980"
     CDS             4871..5836
                     /gene="miaA"
                     /locus_tag="PLH85_04980"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNB63945.1"
                     /translation="MMPDSASLPPAVLLMGPTASGKTACALALARHLPVEIVSVDSAL
                     VYRDMDIGTAKPDAAERAACPHHLIDLISPEEAYSAARFCEDARRLMAEISARGRLPL
                     LVGGTMLYYKALRDGLSELPEADPELRRAIEDTAARDGWPVLHAELARLDPDAAARLA
                     PHDAQRIQRALEIVRLTGAPLAEAYARRAAAVATHRFIPVALTPSDRSVLHARIAQRF
                     DAMLAAGFVEEVETLRRRYVLTPEMPSMRCVGYRQALEYLEGRVDHATFRDKGIFATR
                     QLAKRQITWQRNFRDTWGNLAELDSLAADLPERLHRHVARSLDAR"
     assembly_gap    5846..5945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6016..6162
                     /locus_tag="PLH85_04985"
     CDS             6016..6162
                     /locus_tag="PLH85_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63946.1"
                     /translation="MADYSADILWERGGQDFLDRRYSRTHRLRFDGSLDIAGSASPHV
                     VRPP"
     gene            6525..7613
                     /locus_tag="PLH85_04990"
     CDS             6525..7613
                     /locus_tag="PLH85_04990"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNB63947.1"
                     /translation="MQRIDTLIPPGRPAARVELLLGDLTALDAEYALDVLVVSAFPGD
                     YLPTPGSLIGALHRKGLSVEALAADKDVDLRSAYACWLSRPLAPTDPGLRFSRILCFE
                     PATRGQPPEVVGDIFRALTPILAERPEIRRIGLPVVAAGDQGYAIPTMLAPLLEAAVH
                     WITRGLPIERLSIVLYDAADVAAARETFERFKAQLTTPVPQDLSRRLSPEADAATPEP
                     ATVAPDYDVFISYSHKNTAASEALEAALRRLQPDLRIFVDRKDLDIGCAWQPEIFETL
                     DRCRKVVALLSPDYLASKVCKEEFNIAWIRAREADQDIIFPLYLFSAALPTYMKYRNF
                     FDCREGDADKIEAASKVLLKALASGPTA"
     gene            7671..8195
                     /locus_tag="PLH85_04995"
     CDS             7671..8195
                     /locus_tag="PLH85_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB63948.1"
                     /translation="MRPPLPLLLVALFVTIGFGRPVQAGDPLPEAAPSGPPESPAASA
                     SEPPPNTTPVPLPLRPADMNWASPSGQDAIRESWMMGGEALAGPYALRVRLTAGARVP
                     VHRHPDDRVVTVLAGTVHAGFGGIFDETRTVAMQTGTVFLVPADESHYLWARDGDAEY
                     QENGVGPTGQSRGW"
     gene            complement(8202..8972)
                     /locus_tag="PLH85_05000"
     CDS             complement(8202..8972)
                     /locus_tag="PLH85_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNB63949.1"
                     /translation="MVSSIGSSSYSSYAVSRPDPAQMASKLFSKLDTKGQGYLSETDL
                     QSAFNQMFTSATGGSSSASSTSGTDSEASQLFKALDSDGNGQVTKSEFTSGLQKLAEA
                     LDNQASSSRMGQAHGAGGMAPPPPPQGDGDGDGDDGVSGAGLTKDQLTSQLQQIGDTD
                     SQRSSLISKIVANFDKADTNGDGKVSAQEAMAYDRSSRSSGTAATASAEGTSSSSNQL
                     SDARLLHRVMELMRAYDVNGDAAGNAATGSSGLTAVSA"
     gene            9485..9559
                     /locus_tag="PLH85_05005"
     tRNA            9485..9559
                     /locus_tag="PLH85_05005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9517..9519,aa:Val,seq:cac)
     gene            9713..10747
                     /locus_tag="PLH85_05010"
     CDS             9713..10747
                     /locus_tag="PLH85_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB63950.1"
                     /translation="MSLRIVPRLLAGLLTAALFAIALPARAGKTLDGIRQKGQLACGV
                     STGVIGFSALDSQGRWQGLDVDVCKAIAAAVLGDAGKVRWVPLNSQQRFTALQSGEVD
                     LLSRNTTWSLTRDAGQGLHFAAITYYDGQGFLVPKKAKISSAKQLKGAEICVQSGTTS
                     EKNLADFFRAQGTKVKPVVFDRFDASLKAFFSGRCQAYTTDASSLAFIRMKEAPKPDD
                     YVVLPDLISKEPLGPLVRRGDDEWFAIVKWVVYALIEAEELGIGQARVDALKTSPDPA
                     IQRLVGSGDDLGKALGLDKDWSARALRAVGNYGEIFARNVGADSPLQLPRGLNAPWNK
                     GGLLYAPPLR"
     gene            10758..11714
                     /locus_tag="PLH85_05015"
     CDS             10758..11714
                     /locus_tag="PLH85_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB63951.1"
                     /translation="MAPRRVRLLVQALIVAACVAALAGLVDTTARHLAERGIRSGFDF
                     LLEPAGFSIGESPLPFAADDILLRAFAVGLLNTLRVALPGAALALCIGIVVGLLRLSA
                     HPLARGLGAAYVHAVRNLPLLLHLLAWYFVLTDLLPAADAPLHPLPGVFLSKSGLALP
                     WWGDDGLELPAPGDFGIDGGARLTPEYLALLAGLALYAAAYIAETLRAAVSALPAGQT
                     LAGLALGMRPRQTFFLVLLPQAWRAALPPVTSQLINLAKNASLGVAVGYPDLVSIAGT
                     TLNQTGRAVECLSIVIAVYLCLSLAGAALSRRLERAALRETT"
     gene            11711..12781
                     /locus_tag="PLH85_05020"
     CDS             11711..12781
                     /locus_tag="PLH85_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNB63952.1"
                     /translation="MIAGRLGRLRRALFGDPVATAISLAILAAVAFTLPPLIRWALLN
                     ARFAPDAAACDALDHAGACWGVVTEKYRLILLGRYPQDEAWRPVVASLLMGGGWLAGA
                     AGVLRGRTLAALLGLAPLAAAALLAGGVGGLAEVPAERWGGLPLTLLITATGLAGALP
                     LGIAIAYGRRARLPVLRGLLTAAVELARGIPLVAVLFMASFLLPLLLPEGTGPGMLGR
                     VLIAIVLVAAAYLSEIVRGGLQALPTGQRDAATALGLTPWQIERSIVLPQVLRAVAPS
                     LANSAIALFKDSALVTVVSLYELTGALGLALAGDPQWRAYQLEAYIFLAAIYWAGCAL
                     LARLAVRIGAPPEAGPGPGPRP"
     gene            complement(12789..14150)
                     /locus_tag="PLH85_05025"
     CDS             complement(12789..14150)
                     /locus_tag="PLH85_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB63953.1"
                     /translation="MKRSILVVEDDPVLNHLLVKTLSRAGFDVSAARAWSEARLCLES
                     DAPELVLLDMNLPDARDFGPLAEVAQTRPVIMLTAFGSINQAVEAMRLGAVDYLVKPV
                     NLDELELVIHRALEGSRLRAGDALRTARSTGPAFSPMVGDSPAMRRLWELVQAVADDD
                     VTVMIRGESGAGKELVAQAIHAAGGRRDGHFVAVDCCTLQETLFESELFGHERGAFTG
                     ADRRKPGLIEAAAEGTLFLDEIGEIGPVVQAKLLRVLETGRFRRVGATTDLRANVRVV
                     LATNRDLARMVREGKFRADLYYRISAFEVTVPPLRERREDIAALVAHFARRRAGNGPV
                     PVFSPEALGALLAYDWPGNVRELRNVVERALILASRTGVVEPGHLPPLQGLGDDGADG
                     QIRIQGEPSLEEIERHYLADLLQRYDGNRRKVAEVLGVSERTAYRMLARYGYRNRLDL
                     PDA"
     gene            complement(14147..16000)
                     /locus_tag="PLH85_05030"
     CDS             complement(14147..16000)
                     /locus_tag="PLH85_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB63954.1"
                     /translation="MSLRAACSLSRRLTLVLMLALLMVSGMLSAGIWWVSREAMRREQ
                     EASAERIARLFEASLHTAMLRRDLGGLAEILSRLGHMQGVASAALLHPDGEIRFASRA
                     TDLGTRRPDLVAGLCLRSGCAESPPRIQPLEADDRLRIAYPIRNQARCAACHGPVDVH
                     PVNGVLVVEFKGLVEGDISGQMRGLLPLGLAALGLFALAVGWVVRREVLVPVARLGDV
                     ADRVAEGDLSARAGEPGVGELGRLGLRLDSMTERLGRMIEASGRQQAYLQSLLDAAPD
                     PMLVIAEDGCIVLANSAYLRLLGLAAGEAIGQPCHRVSRGLSEPCPTTLVRCPLAELR
                     ARPEPLRTVMSLRRRDGSEVSVEIHAAAVHTPDGRRMVVEAIRSLDDQVRFSQEQRLS
                     AVGLLANGVAHEIHNPLASIRLALQSSLRGLRDGTMSGEDVTEYLVLVDKEIDRCVAS
                     TQRLLTLSHPPGETRPVDVRAAVEDTLALLEAERQTTGVSVQICLEPPGARVMADEAE
                     LRQVILNLVQNAFHAMPRGGQLRVALGREGADYHLSVSDTGVGIGADDQALVFMPFYS
                     RRADGQQGSGLGLAICKAIVERHGGRIGVDSTPGVGSTFHVRLPAADGESP"
     gene            complement(16042..17049)
                     /locus_tag="PLH85_05035"
     CDS             complement(16042..17049)
                     /locus_tag="PLH85_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB63955.1"
                     /translation="MLNIDGDACVHARSATSRCTACVDACPRGAWTVDWREVAFQPDA
                     CDGCGVCVPACPAGVLELAQPPRLRPDGAGCACLVCERCADDGTLEADALRLPCVHGL
                     SEARILQAHGRGLRRLELRTGDCGTCPRGGGQPLTQRLAALNRALQARGAAPVVLAGT
                     EDAVRGVRPEDARRSFFRRFFERPGAALAEGAGGNAEGSDRRRDALERLARLGPGPGL
                     WSVVLDPARCNGCGVCATLCPELALGWLEGGGQAALRFDAARCTGCQLCVEVCDRDAL
                     RPAAVDEAWPGGPQHFFQVKPCRRCHAPIRRGATASEDADLCPICAVATVGRSGRLVV
                     D"
     gene            complement(17065..17718)
                     /locus_tag="PLH85_05040"
     CDS             complement(17065..17718)
                     /locus_tag="PLH85_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNB63956.1"
                     /translation="MSATDVLPVAAGIAVPAGLLCAAEDLAALAWFHEAERSPALIAA
                     LHEGGFPCGLGVLPADAPALLRFAAAVADVAGVRPDDAPGLADELAADFAAIYLTHAL
                     RASPCESVWRDEDHLMLQGSVFDVRARYQAHGVVARNWRLLPDDHLSNELAFVAHLLG
                     SAQADAAAHFLDAHLLVWLPDFAERVASRAATRFYATLADVTLAAVRAVRERLETGR"
     gene            complement(17715..18407)
                     /locus_tag="PLH85_05045"
     CDS             complement(17715..18407)
                     /locus_tag="PLH85_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB63957.1"
                     /translation="MADKKSIRKLSAEARAEVPAAQARRDAATYEPVKPARQLGFIHN
                     NMDCIGCRACEIACKDKNGLGPGPRFRRVMYVEGGQFPDVFAYKVNMSCNHCAEPACL
                     PTCPTGAIWKRADTGIVDIDSTLCIGCRRCEASCPYGAPQFDPVDNLVKKCNMCVDEI
                     DAGRKPYCVMACMMRVLDVGPIDQLRAGTYATKAMAPGEEPVRQVKHMASIELTNPSI
                     VFIPHPKGRVEE"
     gene            complement(18425..20998)
                     /locus_tag="PLH85_05050"
     CDS             complement(18425..20998)
                     /locus_tag="PLH85_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB63958.1"
                     /translation="MNSVLDAGVTPSATETTRRDFLKVMGVGAGLAGLGGLTMTPKDA
                     KAFAYEPYYTDDQLTTVVTSCAHNCGSRHMLVAHKKGDVIVRLSSDDGRYQKGGFFGK
                     DTVDEPQLRACLRGRSYRSRLYSPERLLYPMMRVGKRGEGKFKRVSWDEALDFVARKM
                     VELKKTYGPTALLDQSYAGASYGVLHKSDQIEGLLGRFLGMFGCRTNSWSVPSYQGTT
                     FSSRITFGTIEDGNEDDAYAHSKLIIMWGWNPAYTFHGGNTFYYMRMAKQRGCKFVLV
                     DPQYTDSASAYDAWWIPIRPNTDAAMLAGMAHVIFTENLHDQAFIDKFVLGMDAGTMP
                     KDYADAESFKDYILGTRDGTPKSPEWAEAICGVSADDIRKLARLYATTKPAALKASWA
                     PGRAAYGEQYNRMAAAVQAMTGNIGKLGGCAEGVGKAWHSESVAYPYDEYANVWYASI
                     KSDRWAHCVINYPKLKREDVGLWPREDELDGQIPNIRGIFWQGSDWFNQLTNINKEIQ
                     AIEKLDLVVCLDSTITPSGIWADVLFPVATHFERHDVALPWYKGHYYIHRPKVIQPLG
                     ESKSDFQIFTELAYRIEALDPTVKNFGKRYNPRATRDYFRNPDAVDEAYLVAWWKGKV
                     QAHQGVTMSWDEFKKRGVYKFTFKEPLVAFRAQVEQGEPFPTGSGKIEIFSPYLAGIT
                     DWTLTQYGAPIPPIPRWIEPFESLNSPKTKDFPFHLISPHPRWRTHSIFHNIPWLRET
                     YDQEVTVNASDARRLGIQTGDTVEVWNERGTCVVPVYVTERCLPGVAVLFEGAWMDLD
                     RDGVDRAGNPDFLTLDEPSPAGAFAYNTVLVNIRKTELAHRPGWDQLGAARSTVFRRD
                     Y"
     gene            complement(20995..21822)
                     /locus_tag="PLH85_05055"
     CDS             complement(20995..21822)
                     /locus_tag="PLH85_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63959.1"
                     /translation="MVVTRRIKQYLRLLATLAAAWTLGPALAAEGHDATGVDYQRARY
                     SPLHFRPAIQQASNEQCLACHSEILTRRPRERSPAGVTAASAKAWYQQLSTYQGAQAD
                     FHRRHLDTGLGKSLMNLQCNTCHDGVDPRDKHPLSSATSPRDGGFRMRKTVMPETTCL
                     KCHGQMNWQVMGLPGPWKESREMFQNNCLLCHEGIRTQRHQVNYLKPEAIETAGKESG
                     DACYGCHGGRAWYRITYPYPRHPWPGMAEETPDWAKGRPTRSEARFVTGASEKGQQP"
     gene            complement(21899..22378)
                     /locus_tag="PLH85_05060"
     CDS             complement(21899..22378)
                     /locus_tag="PLH85_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63960.1"
                     /translation="MKSFGKIVSCSALALALCAALPAVAKDEQKGMSGMSGMSGMEGQ
                     KGMSGMSGMSGMEGQKGMSGMSGMEGQKGMSGMSGMSGMQGQKGMSGMSGMEGQKGMS
                     GMSGMQGQKGMSGMSGMEGQKGMSGMSGMSGMSGNQHKKHKQQGMSGMSGMSGMEGK"
     gene            complement(22489..22788)
                     /locus_tag="PLH85_05065"
     CDS             complement(22489..22788)
                     /locus_tag="PLH85_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63961.1"
                     /translation="MIRSLMVLSAVAMSVVLSAPVSAGAFVAGLQPDRRPAGAPHITT
                     FVLTPEMKAVRLTGISLPLPGNVEVIAQQGAWYSPMFRPGMTGPYDLRRWHGSRE"
     gene            complement(23383..23598)
                     /locus_tag="PLH85_05070"
     CDS             complement(23383..23598)
                     /locus_tag="PLH85_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63962.1"
                     /translation="MLNKFIALAIASAFATASFAEAPAAAPAAAPAAAATAPAAEPAA
                     KPKAKKHHHHHKAAAPAAPAAEAAPAK"
     gene            complement(23733..24128)
                     /locus_tag="PLH85_05075"
     CDS             complement(23733..24128)
                     /locus_tag="PLH85_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNB63963.1"
                     /translation="MPIIQLKISAPKSLEMTHRIAGMLLDLTSRILHKKPEVTAITID
                     YVAPEDWIVGGRTLAEQRRHSFFFDIRITDETNTKDEKARYIREAFDAFAGLLGDVHE
                     ESYIHVHDVRAASYGYGGRTQEFRYHRPG"
     gene            complement(24180..24596)
                     /locus_tag="PLH85_05080"
     CDS             complement(24180..24596)
                     /locus_tag="PLH85_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB63964.1"
                     /translation="MFDQCLYFNTTALARRLEREWADAFRPFGLTPPQAFLLRAVVAE
                     PGRCPGELAAFLAISRPTATRALDGLAAKGLVERRSSPRDGREATVFPTAEAERIGPA
                     VNEAAGAVTTRLKTVLGEAVFVDALAQVRSVRGALG"
     gene            24769..26772
                     /locus_tag="PLH85_05085"
     CDS             24769..26772
                     /locus_tag="PLH85_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNB63965.1"
                     /translation="MKLHAFVAMPFGTKPDAAGNPVDFNAVYDDLIRPALEKAGLDVF
                     RADEEPRAGDIRADMFQELLMADLVLADLSIDNPNVWYELGVRHALRARGVILLQAPR
                     DRQPFDIYTDRKLHYRLKDGRPDPATLDDDRKAITDMATATLKAWHGRKVSPVYALLP
                     NLQEPDWKSLRIGDAREFWERHDAWCALIERARDDNRPEDILVLADEAPITALRVEAR
                     LAAGNALLQGRHFNFALEQFDLAHRFDPDNLTVSRKRGVCLQRTGRAAEAQDLYRDIL
                     AAHPDDVETWSLLGRVDKDAWVTAWRTPGSTPEAMRDAATYEDALLRAAIDSYAAAFR
                     AVPGHFYSGINAVTLMALYQDLTGDTRYAADQAVMAGGIAWSAHSTLTARPCGQAPQD
                     LYWAAATLGDLAVLRGDAPAVRAAYKEAIVHADNDWFALDSTLGQLRLLAELGFRPDN
                     VAAGIATYERALARLRPPEKSWQPGKVFLFSGHMVDAPGRKEPRFPADKEPVAAAAID
                     QALSDEGASDQDLALTQGAAGGDLLFAEAALKRGLRLQLLLPLPEPDFIEASILRSAD
                     GETWRKRYFQVRDHALCLPPRVMPDVLGPLPVDHLGNSMNEFERCNQWLLYSALACGI
                     ARTRFVCLWNGTGGDGPGGTQHMVQEVRRRTGKVTWLDTRKLW"
     gene            26795..27286
                     /locus_tag="PLH85_05090"
     CDS             26795..27286
                     /locus_tag="PLH85_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63966.1"
                     /translation="MLRPLPLLSLAALLLFVGVLWSDQPLVEAGHSMVELQFAGSAER
                     AARILDTWRTAGVEDAIRLNQQIDFAFLLTYPAALSLACRSLAGHLGPGQRIGHFLTR
                     AVFACTVLDGAENLAIQVMLDHGARDSMARLATACAIPKFALAAWAIGWCLWALLRRV
                     RLP"
     gene            27283..30093
                     /locus_tag="PLH85_05095"
     CDS             27283..30093
                     /locus_tag="PLH85_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB63967.1"
                     /translation="MSTQAHGHPHGPRQRRSFAARLFGYDLFISFALGHPPRGTQSYA
                     SDLARRLRERDFAVFYSEDEAAPGGELDSTLRNALARARILVVIANRGTLADPRWVRR
                     EVEEFRALHPGRPIIPISLDGALQDPQLAAACKDWLDMSDTIWLDDTAEAAEHGLVSP
                     AVIDRLVLAPTQMRSSQGWRITVAAVSLTLIGLTASSIWSEHRARTNENSARQEAEHA
                     RRAERQAREELRVSTGLRLRAESSAMLGGLRPEPDERALLQLLAALHLGRGDDTLDPD
                     AEGELLGGLRHGRNLVRMSPAAHPILSLALSRDGTHLVTGGEDATLQLWNALTGDPVG
                     PPLRAHEDAVRAVDFSPDGQWLVSASTDNTLRLWQVGRDGLQPAPLPPLKGHTDSVRT
                     VAFSPDGRLIASGSDDNTVILWDARSGTPLPRRLTGHGDSVTGLAFLPDGRRLVTASN
                     DMSLRLWDIPSGRPLGPPLSGHTDSVKCVAVSPDGRRIVSGAWDNTLRLWDPVTRRPI
                     GRPLTGHRALVRSVAFSADGRRIVSSSDDGTLRLWDARRGTLLAPPLRGHQGEVTSAL
                     FARKGQRIISGGMDRSLRQWDPRTTSPLGEAQSAGSAALLSVAVSPDGKRLAGSGEDL
                     RGRLWRVASARTAANARGIALSGPSAAFYALEFNADGARLAGGDAAGQLWQWDGRSGE
                     TLAAPHPAHARPISALAYSPDSRRIATGSWDNTIRIWDATSGAPLGPPLTGHRGWIAA
                     LAFSPDGRTLVSASADTTLRRWDVATGHPLGAPLSGHTNWVLAVAFSPDGRNLASAGA
                     DRQIRRWNAVTGAGIGTPSGGHESWITSLAFSPDSRYLVSAGWDRSLRLWRTDSGLPV
                     GIPLQGHRDWIQKVAFRVDGLRIHSADRAGALHLWAAPRHWPEMLCAKLSRNLSRAQW
                     KAWVSPDIDYVVQCPGLPVPPD"
     gene            30115..30492
                     /locus_tag="PLH85_05100"
     CDS             30115..30492
                     /locus_tag="PLH85_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HNB63968.1"
                     /translation="MHEPADIHRLFAERFAAADLDGLLELYAPDALLLPQAGSEVRGH
                     AAIREALGGFIALGGRFTMAPPRCLRAGDIAVLHADWQLSASGPDGQPVHLGGHTADV
                     VQRQVDGTWRLIIDNPWGDAVTA"
     gene            30614..31771
                     /locus_tag="PLH85_05105"
     CDS             30614..31771
                     /locus_tag="PLH85_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB63969.1"
                     /translation="MTQTLADRIRAPGPKKILSCDGGGILGLISVEILAKLEADLRVR
                     TGKPDLLLGQWFDFVCGTSTGAIIAACIAAGMSMERIRTFYIESGQQMFDKASLFRRL
                     QYSYNDEPLARKLRDEFTRALGDDTTLGSPGLRPLLMMMMRNATTDSPWPVSNNPFGK
                     YNQPARKDCNLRLPLWQLVRASTAAPTYFPPEVVTLGQGDDAYSFIFVDGGVTTYNNA
                     AFMAFQMATATPFGIRWATGTREMLIVSVGTGSVAAARPDLEADDLTLIENAKTLPGA
                     LMNAAAAGWDRACRLIGECRFGALIDSEFGDMVVPPGGANTYSGSKQFAYVRYDPDVS
                     QAGLNALGLSGIKAADVQTLDSVAHIPDIRTVGKTYADRNVKLEEHLRGFV"
     gene            31853..33301
                     /locus_tag="PLH85_05110"
     CDS             31853..33301
                     /locus_tag="PLH85_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4071 domain-containing protein"
                     /protein_id="HNB63970.1"
                     /translation="MTPKTCFVIQGFGKKTDYTDGRVLDMDASYGVIKDAVEAAGLVC
                     LRADEIPHSGTIDEPMYRQLLQADLVIADLSTYNVNAAFELGVRYGLRPRATLIVAEE
                     GFKNPFDVSHLVIRRYKHLGEDIGVKEARRFADELKLAIAAILSADQADSPVYTFLPG
                     LQPPALPPPAPRAAPVQAQAEAPTRAGGFDLSKARDWLDGLFHTSAPEPGTDAAADTA
                     GPPTLSRWEPPEVPAPAAPRSAKSLLDEALAALQSGEDTPPDFIRARRLLEAVREQRP
                     NDPFVIQQLVRAISGAARPTPQAALREALALMNELGPATTNDPETLSLWGSVHQRRWE
                     LDAQPADLEEAIAGFERGYFVKQDWQTGSALAFLLDRRALEHLKSGASDEAVADGVQA
                     RRLRREVLRLAGPHIDHPADDAAKRFSVMCAVWEAHLGLGARSEADMLDARLDALPVP
                     APLNARRKAHGRTLVAQLDAFTRLRGGAAGST"
     gene            complement(33544..34359)
                     /locus_tag="PLH85_05115"
     CDS             complement(33544..34359)
                     /locus_tag="PLH85_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB63971.1"
                     /translation="MPAEVVSVAFDCLPRLAPSADEPVTVNQRRVPPDVAVPPHRHPW
                     GQLAYPVSGALRIAAAGIAWIVPPFRAVWIPPEMEHDVVMIGQVDLRTLYIAREVAPL
                     ALDRCAVVDVSPLLRNLIEALAERRTDRRHRLMMELVLEEIRVAPPLSLGLPLPKDRR
                     LKQLCDRLMADPADDRGLAEWAREVAASERTLARLFRAELDTSFGAWRQQLRLARAVD
                     DITRGVPLARVAAEVGYASQAAFSAMFRRALGVPPSRFSGAPKERRGGDPEQG"
     gene            34495..35697
                     /locus_tag="PLH85_05120"
     CDS             34495..35697
                     /locus_tag="PLH85_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB63972.1"
                     /translation="MSSDAALSAPPRHDAFVIALIGSVHGVSHFFHLLLPPLFPWLMP
                     EFGLDYSRIGTTMTVFFVVSGVGQALAGLAVDRFGPLRVLASGIGCFVLAGLTLSRAN
                     GFADLMIVAALAGLGNSVFHPCDFTVLNRNVSVPRLGHAFSVHGLSGNVGWALAPLFM
                     TGIATAAGWRTAALAAGLLAIPALALLLAGRRHLVDATPPEHQDGRRASTAEILRVGP
                     VWLCFAFFFFLTTAFGAIQAFGTPLMQHLYELPLGIAAGTVSSYLFAGAVGIFVGGFL
                     ARSVQQDRVIALALSAAAAMAVLLASAQVPAGAVLPVMACIGFLSGLAGPSRDLLVRR
                     AATARFGQAAFGRVYGFVYSGLDTGLALAPLVFGGFMDSGHYGTVLVGIALLQVAAVF
                     TALKVGRG"
     gene            35697..36269
                     /locus_tag="PLH85_05125"
     CDS             35697..36269
                     /locus_tag="PLH85_05125"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNB63973.1"
                     /translation="MARIFSFPPVAAPDARVLILGSMPGEASLKARQYYAHPRNLFWP
                     ILGELVGARPDLPYPDRLAALRQARIALWDVLQSCERDGSLDSAIDPQTRVVNDFGDF
                     FTRHPHIGWIFFNGREAEKTFRKHAIHVCDAERFRYTLLPSTSPANASIPPAVRLDAW
                     RVIVDAPRRVSAPHPGSMAGAGATLQGSST"
     gene            complement(36251..37984)
                     /gene="phaC"
                     /locus_tag="PLH85_05130"
     CDS             complement(36251..37984)
                     /gene="phaC"
                     /locus_tag="PLH85_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNB63974.1"
                     /translation="MSSREPPAPFSTARKSLKKIHDSVEASIDPIGMTAPIVHAQLAW
                     MAHPQELADALAKVSTSLWALQWHSMRRVFGLPSEDPEKPHGDDTRFADPVWGESPTW
                     DLIKEWYLVFTHHMQDMLYDTPGLSQKERRRAAFWWRKWLNAMAPTNFLMTNPVALRL
                     AMETRGESLVKGFQNFLADMEAGNVRMTDPNDFQVGVNLATTPGKVVFRNRLLEVIHY
                     TPSQPKIHARPVVIVTPWINKFYILDLTSRKSMIRYLLDQGLDVFITSWKNPGEDMRD
                     VRFDDYLTEGVHMLVETARAMSGADKVHAVGYCIGGTVLTTYMAWANRHFKAEDVPVA
                     HFTLFTTLVDFHKPGDIEVFVDEGSIRYLTQTMAQKGYLDGAEMASAFRLLRSNSLIW
                     HYVVHGWLYGEKPAPFDVLYWNMDSTRMPYAMHAWYLRELYLHNRLIEKDALTVAGEP
                     IDLGAIAQPVYAVAAEDDHIAPWQQTFRTMNYVAGPKRYVLSSSGHILGIVNPPVNPP
                     KRRYHVGPARRSDAADGWLAKAAEHPGSWWTDWMAWLKPQCGDLVDARPVADEAHPAL
                     ADAPGTYVLEP"
     gene            38154..38597
                     /locus_tag="PLH85_05135"
     CDS             38154..38597
                     /locus_tag="PLH85_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB63975.1"
                     /translation="MFKHILVPTDGSELSSATVTRAVTFAREAGAKITFFYAQPDFPM
                     PIYGEGALIDPTTPEQFSKAAAAEADAILAKSRELATVAGVECAGVTVVSEVPYEAII
                     EAAEKSACDLIFMASHGRRGLSGFLLGSETQKVLTHSKIPVLVYR"
     gene            complement(39087..39329)
                     /locus_tag="PLH85_05140"
     CDS             complement(39087..39329)
                     /locus_tag="PLH85_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63976.1"
                     /translation="MRKLIEVLWPSFLVAGLAEIVFFTVVNPQELYLFGEPVHFSATA
                     TYSIGFFGFWLVCAASSLMTLFFQRTSAEINKLDGV"
     gene            complement(39389..39532)
                     /locus_tag="PLH85_05145"
     CDS             complement(39389..39532)
                     /locus_tag="PLH85_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNB63977.1"
                     /translation="MAWKELFSTDIGMLSLFTIGFVIVMGGYLYRYAKSHMAEDERRA
                     RGG"
     gene            complement(39547..40080)
                     /locus_tag="PLH85_05150"
     CDS             complement(39547..40080)
                     /locus_tag="PLH85_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNB63978.1"
                     /translation="MNTSTLNTIRDDKPWYRERWPWLLMAMPATAIVAGFITLWLAIT
                     SFDGLVADDYYKQGLAINQTIGRAVVARDLGLVAQVRFSADGVAIALTAKPGIALPER
                     VLVTIAHPTRGGLDQHLNLQGQAGAYRGALAQELVSAHWKVLLEDESRSWRLTGTAFL
                     PTETEIRLDSADLKPVD"
     gene            complement(40077..>40569)
                     /locus_tag="PLH85_05155"
     CDS             complement(40077..>40569)
                     /locus_tag="PLH85_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HNB63979.1"
                     /translation="PKGLIRYTTENAMRRHWGRKEILGHVMRPRILIYSSVLVALVIA
                     LVWGLATRSTLRVDVIKDRSTLSREVEGGLIANVYNLQVMNMTEKPRTFTLSVSGLDQ
                     IRISPNKPFEVDAAGLKAVTVEVAAPPESGKPGSNTIYIEVKAADDQTVAVREKTAFL
                     LPQ"
BASE COUNT         6480 a        14795 c        12815 g         6379 t
ORIGIN      
        1 cgtatcaatg ccgtgatagc tccgcctcga tgattccaga tatagcaggt tccatgacga
       61 cgccgatggg aatccccgtg ggcaaaacgc cacccgcaat tggaacaaat cccgggcgcg
      121 aagtactcca cgccccttca atgatcgacc gagttgtata gacaacaagg tatcgtaccc
      181 tggccgtttg cgcgttcagg ggaatgcgcg cctggagcga ggctgaacgc ggaggcatca
      241 tcgatgccgg gaccgggacg aagagtccgc gctgaagatc ggcgaggacg ttgaaattct
      301 cgtcgagcag catgaccgct ggcgaaaaaa tataaaactg cccagtgccg ggcaatccac
      361 tcggcgtcac cacgcttcgc aaggccacgg tgttctgcat cgatggcgcg gacagctccc
      421 acgcaacgaa tcggcttcgc ccttcaggaa agtcaaacac tggactattt gaactgatcg
      481 tatcctcacc acgctgaccg gagctcaatt ttttataggg caagctcgcg aggctggcgc
      541 agcacggacg caattcactc aggacttcgc cataagcgac gccgcgctgc tcaggagaag
      601 tcatatccct gactgtcagg cggtcaatgg acgccgcatc cagctgagga tttcggcgga
      661 ggagttcggc tttggcgccc tgaagatcat tgacaaattc cggctttata tcgcgcatat
      721 tggcgctgcc gacgctgtca aaacgcttct tcccgtgcag aatcagatca ccgaagtaat
      781 aggatcttcc ggagcgctcg agagcaaagc tataggaaac gacacctccg gcgtagttgt
      841 ggagcacgat gctgacttga tatcgcccgg gcggcaacgc ccagtaaaag cgtccatcat
      901 tctcgaattt ctgcgccagc atgcgcggtt cgtcggctga cgatatgtcg cgaatgacga
      961 cgggcatgga gaacggtgaa atgacaagcc tttcgccctc tcgatcaagg acgatccggc
     1021 caaagaccag ggcctttccg tccagcctgg acactccatg atcaaatgcg acattggcac
     1081 agccactcag caacacgctc cagacaaggg caaaacagaa ccaccgggat gaccttccaa
     1141 aattattgag cattgccgac actcccacga aatatccgcc catacaataa agccatacgt
     1201 gcaacgatct ccgctcgaat tggctttgcg ctccgacggt cttgaccggg atcgtcgcac
     1261 ttgacgaggc acctgcgtgc acgcgccacg tctgcgggca cgtggcagaa cagtaccgga
     1321 gcggatgcag tgcccttcgc cacgttttct gccaaaatac gccccttctg gcctccttcc
     1381 cgccaatttt tgcatccgct gcgccccacg aggccttccg gccctcgcct cccaccgtga
     1441 aacttgaccg tctcctccag gctcagggct tcggcagccg ccgccaatgc cgccaactcg
     1501 tcgaactcgg cgaggtgcgc gtgcgtggcg atgtcgtgga cgacccggac gccgccatcg
     1561 acacagccgg tctcacctat gaagtcgccg gcgaggcctg gatctaccgg gaaaagacct
     1621 acctcgcgct gttcaagccc gccggcttcg agtgctccca cgagcccaag caccaccgca
     1681 gcgtgtacag cctgctgccc cacccgctgg tggtgcgcaa cgtgcagagc gtcggccggc
     1741 tggacgtgga caccaccggt ctgctcctct tctccgacga cggcgccttc atccacaccc
     1801 tgtcctcccc caagaagcac gtgcccaaga tctaccgggc cgaactcgcc gagcccgccg
     1861 ccgacaccct cgtcccgcgt ctgctggccg gcgtcgacct gatcgacgag ccccgcccgg
     1921 tcgccgcgct tgacgcctgc ctgcgcggcc ccacgaccct cgaaatgcgc ctcgatctgg
     1981 gcaagtacca cgtggtcaag cgcatgatcg cggcggccgg caaccacgta cagaccctcc
     2041 accgcagcgg tttcggcggc ctcagcctgg gtgaaggccc cctcgccgga cttgccgaag
     2101 ggcagttcat ctacctggac gccgacgccc tgcgtcagct ccgcgccgtc tgaaagggac
     2161 gccatgtccg acaccctccg ccgccgtccc atgctgccca cgcgcacggg caccctgcgc
     2221 ctcaagtccg gcacacctac cccggcgggc gaccgccccg cgacccagga ctggcgcccg
     2281 cccgccgccc gccgcggcaa agcgtccatg cccgagcagg ccaatactga atccaaccgc
     2341 cgcccgcccc gcgaagggga cgtccgcggc gacgtgccgc cgcgccgctt tggcgacgag
     2401 cgcccccctc gcacagacga gcgccgccct ggccgcaacg ccgacgaacg cttccgccga
     2461 gacgacaacg gccacccgcc ccgtgccgat ttccgcaaac gtcgggacga cacggaccgc
     2521 gacccgcgcc gcttcgacga cgaccgccgt cctgcccgaa gcgacgacac gggcgtccgt
     2581 cgtgaccgcg acacccgtcc ggcccgcccc ttccgcctgc cggacgaacg ccgccgcgaa
     2641 gccgaagatg gccgtccgcc ccgtggcgga ttccgcgacc gccgggacga cgccccggac
     2701 cgcgccccgc gccgcttcga ggacgaacgt cccccgcgga acgatgacgg tcgccctggg
     2761 cgcgcggccg acgagcgctt cagacgcgac gacgaccgcc gtccgccccg tggcgatttc
     2821 cgcgagcgtc cccgccgcga ggacaccgac cgcgggccac gccgcttcgc cgatgaacgc
     2881 cccgcccggg gccctcagga ggcgcgtccg ccgcgttccg acgcgccccg ccgtccgggc
     2941 cgcgaagacg accgccgccc gccccgcgat cgggacgacc gtcccgcccg cggcgccgac
     3001 gcgcgcggcc gccgcgatga ggcccgcccc ttctctggcg gcaatccgga cgcccccgcg
     3061 cccgccggcc ggccgcgtcc tgtcgcccgt cccgacgcac cgccccgcca cgaacgcaaa
     3121 ccctggaacg gcgaagtccg cccggcgccg gccccgtcgc cccttcgcat cgacggctca
     3181 atccgcctct ccaaacgcat gaccgaactg ggtctgtgct cccgccgtga agcggacgaa
     3241 tacatcgaga agggctgggt gagagtggat ggccgcgtgg tcagcgagct gggcgcccgc
     3301 gtcaagcccg gccagacgat cgaactggat cgccgcggcc agaccgccca ggagcgccgc
     3361 gtcacgattc tcctcaacaa acccgtcggc tacgtgtccg ggcaggccga ggacggctac
     3421 gaacccgcca gcgtgctcct caccccggag aaccgctggg ccgaagatcc caacgagacc
     3481 cgctggctgc gcacccacct gaagggcctc gcccccgccg gccggctgga catcgactcg
     3541 gtgggcctgc tggtcttcac ccaggacggc cgcatcgccc gcgccctgat cggcgacgat
     3601 tcggatgtgg aaaaggagta ccacgtgcgc gtcgaaggtc gcctcccgcc ggagggcctc
     3661 gccctgctca accatggcct gtccctggac ggccaggccc tgcgtccggc caaggtgagc
     3721 tggctcaacg aggaccagct gcgcttcgtg ctgcgggaag gcaagaaacg ccagatccgc
     3781 cgcatgtgcg aactggtggg cctgaaggtg gtcggtctca agcgcgtgcg catcggccgc
     3841 gtactcctcg gtccgctgcc gccggggcaa tggcgctacc tggcagcggg cgagaagttc
     3901 tgaggccccg cctcgtcgcg cctcagtccg cctccacctg acgcagacgg aatccccgcc
     3961 gcgcaagttg tgccaacagg ccggaatcct ggccgaaatg tcccgcgccc acggccacga
     4021 agggccgcgc accgggcgtg aggcgcccga cgagcgtctc ggccatctcc cggttgcggg
     4081 cgtccagcat gtcctccagc agtggcgcca tcgcggggct ggacgtcacc tcccgcatca
     4141 agtggtcgag ttcgcccacg tcgccggccc gccaggcctg cacgagggca ccaaaatatt
     4201 cccgcccggc ctcgttctcc accagatcga ccacctcctc cacgcccgcc gcctgggcag
     4261 cgtcgccgcc ggccgacagg gccgccagct ggcgctccac cgtttccagc ggacgcaggg
     4321 gcaggccgcg ggcctgggcg gcgcgggcca gccacaagtc gatcccctgc tccgtgacga
     4381 aaccagcctg ggctgccgcc cgcagggtga gcaccgtgga cgccatccac ggctgcaggc
     4441 gttgcaggac ggccggcgga atccccagca ggcgggccgc cgcagtaagg cgcgggcgca
     4501 gggccgccgg cagccggtca tccaggcgct ggtcctccgg cagcacgccg gcacgggcca
     4561 gcacgcccgc cagggccgga tcctcaggat ccagttccag cgccaggctg tcggaccggg
     4621 cgaaggcttc cgtcactcgc gccggcagcg gaaagcaggc cgcgtcgcag acgtggatcg
     4681 tgccatacag gtaggcgcgc accgtgccgg cgtcgtccag cacctcccac aggaagagcg
     4741 gccccctcgc cgcagccagc ccactccaca ccatcgccag cgccacgacc gcccaccgcg
     4801 ccgcactgca ccacccgttc cacgccttca tgccactccc gctacaatgc gccaccctga
     4861 tccagtcgcc atgatgcccg attccgcctc cctgccgccg gccgtcctgc tgatgggccc
     4921 caccgccagc ggcaagaccg cctgcgccct ggccctcgcc cgccatctgc cggtggagat
     4981 cgtcagcgtg gattcggccc tggtgtatcg cgacatggac atcggcaccg ccaagccgga
     5041 cgccgccgag cgggccgcct gcccccatca cctgatcgac ctgatcagcc cggaagaggc
     5101 ctattccgct gcccgcttct gcgaggatgc gcgccgcctg atggccgaga tcagcgcccg
     5161 cggtcgcctg cccctactgg tgggcggcac catgctctac tacaaagccc tgcgggacgg
     5221 cctgtcggaa ttgccggagg cggaccccga actgcgccgc gccatcgaag acactgccgc
     5281 gcgggacggc tggccggtcc tgcatgccga actcgcccgc ctcgacccgg acgccgcggc
     5341 gcgcctcgcg ccccatgacg cccagcgcat ccagcgtgcc ctggagatcg tgcggcttac
     5401 cggcgcgccc ctcgccgagg cctacgcccg ccgcgcggcc gcagttgcta cccaccgctt
     5461 catcccggtc gccctcaccc cctcggaccg cagcgtgctc cacgcacgca tcgcccaacg
     5521 cttcgacgcc atgctggcgg ccggcttcgt ggaagaagtc gagacgctgc gccgccgcta
     5581 cgtcctcacg cccgagatgc cctccatgcg ctgcgtgggt taccgccagg cgctggaata
     5641 cctggaaggt cgcgtggatc acgccacttt ccgcgacaag ggcatcttcg ccacccgcca
     5701 gctcgccaag cgccagatca cctggcagcg caacttccgc gacacctggg gcaatctggc
     5761 ggaactggac agcctcgccg ccgacctgcc cgagcggctc caccgccacg tggcgcggtc
     5821 cctcgacgcc cgctgaatcc cgaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnncgagc ggctccaccg ccacgtggcg cggtccctcg acgcccgctg aatcccgacc
     6001 gaaaggcagc gaccgatggc cgactactcc gccgacatcc tgtgggaacg gggcgggcag
     6061 gactttcttg accgccgcta cagccgcacc caccgtctgc gtttcgacgg cagcctggac
     6121 atcgccggct ccgcctcgcc ccacgtggtg cggccaccct aggccgaccc gtccgccgtc
     6181 gaccccgaag aagccttcgt cgccgcgctg gcgagctgtc acaggctgtg gttcctgcac
     6241 ctggcctgcg aaaagggctt ccgggtggat cggtacgccg acggcaagct gtggatgagc
     6301 cacgtcaccc tgcgtccggc cgtgcgcttt gccggcacgc cgtgtcccga cgcagcagcc
     6361 ctcgccaccc tccaccacgc ggcccacacc gaatgcttca tcgcccgttc ggtcaccggg
     6421 ttgagccggt gctcgatccc gcctgagccc cgccgccacg acaggccgcc gctgccgggc
     6481 tatgctgaaa tgatgacatt gccccgcaca acggagatcc caccatgcag cgcatcgaca
     6541 ccctcatccc gccgggcagg cccgctgcgc gggtcgaact cctgctcggc gacctgaccg
     6601 ccctcgacgc cgaatacgcc ctggacgtcc tcgtcgtctc cgcctttccc ggcgactacc
     6661 tgcccacgcc cggctccctg atcggcgccc tgcaccgcaa gggcctgtcg gtggaagccc
     6721 tggccgccga caaggacgtg gacctgcgca gcgcctacgc ctgctggctg tcccggcccc
     6781 tggcgccgac cgatccgggc ctgcgctttt cacgcatcct gtgcttcgag ccggcgaccc
     6841 gcggccagcc gcccgaggtg gtgggcgaca tcttccgtgc cctgacgccg atcctcgccg
     6901 agcggccgga aatccgccgc atcggcctgc ccgtcgtggc ggcgggcgat cagggctacg
     6961 ccattcccac catgctcgcc ccgctccttg aggccgccgt gcactggatc acccgcggcc
     7021 tgcccatcga gcggctgtcc atcgtcctct acgacgccgc cgacgtggct gccgcacgcg
     7081 agacctttga gcgattcaaa gcgcaactga caacccctgt cccccaggac ctcagccgac
     7141 gcctctcgcc ggaagccgac gccgccacgc cggaacccgc cacggtggcg cccgattacg
     7201 acgtcttcat cagctattcc cacaagaaca ccgcggccag cgaagccctc gaagcggccc
     7261 tgcgccgcct gcagcccgat ctgcgcatct tcgtggatcg caaggacctg gacatcggct
     7321 gcgcctggca gccggagatc ttcgaaaccc tcgaccgctg ccgcaaggtc gtcgccctgc
     7381 tgtcgcccga ctacctggcc tccaaggtct gcaaggagga gttcaacatc gcctggatcc
     7441 gcgcccgcga ggccgaccag gacatcatct ttcccctcta cctgttctcg gcggcgctcc
     7501 ccacctacat gaagtaccgc aacttcttcg actgccgcga aggcgacgcg gacaagatcg
     7561 aagccgccag caaggtcctg ctgaaggccc tcgccagcgg tccgacggcg tgagcggcgc
     7621 ggtatcatcg ccgtcacccg cccgtcgccc acaattcccg gaggcccgtc atgcgcccac
     7681 cgttgccgct gctgctcgtc gccctcttcg tcacaatcgg gtttgggcgg cctgttcagg
     7741 caggcgatcc cctgcccgag gcggccccgt caggcccccc ggagtccccc gcagccagcg
     7801 ccagcgaacc gccgcccaac accacgccgg tgccactacc cctgcgcccg gccgacatga
     7861 attgggcgtc cccgtcggga caggacgcga tccgcgaaag ctggatgatg ggcggcgaag
     7921 cgctcgccgg cccctacgcg ctgcgggtcc ggctgacggc cggcgcacgg gtgcccgtgc
     7981 atcgccaccc ggacgaccgg gtggtcaccg tgctcgccgg caccgtgcac gccggcttcg
     8041 gcggcatctt cgacgaaacc cgtaccgtgg ccatgcagac gggcacggtc ttcctcgtcc
     8101 ccgccgacga atcccactat ctgtgggccc gggacgggga cgcggaatat caggagaacg
     8161 gcgtcggccc gacaggccag agccggggct ggtgagatcc ctcaggccga cacggccgtg
     8221 agccctgacg acccggtggc ggcgttgccg gccgcatccc cattcacgtc ataggcacgc
     8281 atcagctcca tcacccgatg cagcaggcgt gcgtcgctga gctgattgga ggacgagctc
     8341 gtcccctcgg cgctggccgt ggccgcggtc ccggaactgc ggctgctgcg gtcatacgcc
     8401 atggcctcct gggcgctcac cttgccgtcg ccattggtat ccgccttgtc aaaattggcg
     8461 acgatcttcg aaatcaggct ggagcgctga ctgtccgtat cgccaatctg ctggagctgg
     8521 ctggtcagtt gatccttggt caggccggcg ccggacaccc catcgtcccc atcgccgtcg
     8581 ccatccccct ggggcggcgg tggcggcgcc atgccgcccg ctccgtgggc ctgccccatg
     8641 cggctgctcg aggcctgatt gtccagggcc tccgccagct tctggaggcc ggacgtgaac
     8701 tcgctcttcg tgacctggcc gttgccgtcc gaatccagcg ccttgaagag ctgactcgcc
     8761 tcgctgtcgg tcccgctggt ggacgacgcg gaggatgaac cacccgtcgc cgacgtgaac
     8821 atctggttga aggccgactg gagatccgtc tcgctgagat agccctggcc cttcgtgtcg
     8881 agcttcgaga agagtttgct cgccatctgt gccgggtcgg gacgactcac ggcgtaactg
     8941 gaatagctgg aactgccgat gctgctgacc atgatggaat cctttcctgt ctgagcgggc
     9001 accgcccgcg ggacaggccg ccgcgtcctg aacggacgcc gaggccctgc ccggtcctga
     9061 gcaacttatc ggccggaaac ccaccggatt cgcggcgatt caggttaagc cgggtaaagt
     9121 tcggttaaga ctccacggcg ccaggacggt cggtggcggc ggtgaatcac cctctgaccg
     9181 gcgcgggatc gtcccggccc tccgacctgg cacatcctgc cggtcgttct ccctgcaggt
     9241 cgccggaacg gcaaggaaac tccatgagaa gccctggctc gccaggggaa gctccggtac
     9301 gcggcgcgcg cgcaggcgcc acccggcaaa tcctgccgcc ctgatccacg ctgcgccgcc
     9361 ctggcgcgga accaccggat tgccgtccgc gcccgaatgg cgggagatgc cgtctattgg
     9421 cctgttttcg cagaaaaggc ttgacggaca gcgggtccac gtctaatatt cgcgcctctc
     9481 ttccgggcgg ttaactcagc ggtagagtgc caccttcaca cggtggaagc cactggttcg
     9541 atcccagtac cgcccaccag attccgaaag ggccacgcca gcgcgtggcc cttttgcttt
     9601 gcaggggctc ccggtccctc gccacactca cgggctttgc acggagcctt cgaagcccga
     9661 taagctggcg ggcattccga ttctcccggc ggttcgatct gccgtccccg tgatgtccct
     9721 acgcatcgtc ccccgcctgc tcgccggcct cctcaccgcc gcgcttttcg ccattgccct
     9781 tccggcccgg gccggcaaga cgctcgacgg gatccgccag aaaggtcagc tcgcctgcgg
     9841 cgtcagcacc ggcgtgatcg gcttttccgc cctggacagc cagggccgct ggcagggcct
     9901 ggacgtggac gtctgcaaag ccatcgccgc cgcggtgctg ggcgacgccg gcaaggtgcg
     9961 ctgggtgccg ctcaacagcc aacagcgctt caccgccctg caatccggcg aggtggacct
    10021 gctgtcccgc aataccacct ggagcctcac ccgcgacgcg ggccagggcc tgcacttcgc
    10081 agccatcacc tattacgacg gccagggctt cctggtgccc aaaaaggcaa agatcagttc
    10141 ggccaaacag ctcaagggcg ccgagatctg cgtccagtcc ggcaccacca gcgaaaagaa
    10201 tctggccgat ttcttccgcg ctcagggcac caaggtcaag ccggtggtgt tcgaccgctt
    10261 cgacgcgtcc ctcaaggcct tcttcagcgg gcgttgccag gcctacacca ccgacgcctc
    10321 atccctcgcc ttcatccgca tgaaagaggc gcccaagccg gacgactacg tggtgttgcc
    10381 ggacctgatc tccaaggaac ccctcggccc gctggtacgc cgcggcgacg acgaatggtt
    10441 cgccatcgtc aaatgggtcg tttacgccct catcgaagcc gaggagctgg gcatcggcca
    10501 ggcccgcgtg gacgcgctga agacctcacc cgacccggcc atccagcgtc tggtgggtag
    10561 cggcgacgac ctgggcaagg ccctcggcct ggacaaggac tggtcggccc gcgccctgcg
    10621 cgccgtgggg aactacggcg agatcttcgc ccgcaacgtg ggcgccgatt cgcccctcca
    10681 gttgccccgc ggcctcaacg ccccgtggaa caagggcggc ctgctctacg cgccgcccct
    10741 gcgctgaagg ccatccgatg gcgccgcgca gggtccggct gctggttcag gcgctgatcg
    10801 tcgcggcctg cgtcgccgcc ctggcggggc tggtggacac caccgcccgg catctggcgg
    10861 agcgcggcat ccgcagcggc ttcgacttcc tgctggagcc ggcaggcttt tccattggcg
    10921 aatcgcctct gcccttcgcc gccgacgaca tcctgctgcg agcctttgcg gtcggcctcc
    10981 tcaacaccct gcgggtcgcc ctgcccggcg ccgccctggc cctctgcatc ggcatcgtcg
    11041 tcggcctgct gcgcctctcc gcccatccgc tggcgcgggg gctgggtgca gcctatgtcc
    11101 atgccgtgcg caatctgccc ctgttgctac acctgctcgc ctggtatttc gtcctcaccg
    11161 atctgttgcc cgccgccgac gcgcccctcc atcccctgcc cggcgtgttc ctcagcaaga
    11221 gcggcctggc cctgccctgg tggggtgacg acggcctgga actgccggcg ccaggcgatt
    11281 tcggcatcga cggcggcgcg cgcctcacgc cggaatacct ggctctgctg gccgggctgg
    11341 ccctctacgc cgccgcctac atcgccgaga ccctgcgcgc cgcggtgagc gccctgcccg
    11401 ccggccaaac cctcgccggc ctcgccctgg ggatgcgccc gcgccagacc ttcttcctgg
    11461 tcctgctgcc ccaggcctgg cgcgcggccc tgccgccggt caccagccag ctcatcaacc
    11521 tggccaagaa cgcgtccctg ggcgtggccg tcggctatcc ggacctggtc tcgattgccg
    11581 gcaccactct caaccagacc ggccgcgcgg tggagtgcct gagcatcgtg attgccgtct
    11641 acctgtgcct gtccctcgcc ggcgccgccc tctcgcgccg cctggagcgg gccgccctgc
    11701 gggagacgac gtgatcgccg ggcgcctcgg ccggctacgc cgcgccctgt tcggcgatcc
    11761 cgtcgccacg gccatctccc tggcgatcct cgccgcggta gccttcaccc tgccgcccct
    11821 catccgctgg gcgctgctga atgcccgctt tgcgccggat gcagcggcct gcgatgccct
    11881 cgaccatgcc ggcgcctgct ggggcgtggt gaccgaaaag taccgcctca tcctcctcgg
    11941 ccgctatccc caggacgaag cctggcgccc ggtcgtcgca agccttctca tgggcggcgg
    12001 atggctcgcc ggcgccgccg gtgtgctgcg cggccgcacc ctggccgccc tgctcggcct
    12061 tgcaccgctg gcggcggcgg ccctgctggc gggcggcgtt ggcggccttg ccgaggtgcc
    12121 cgcagaacgc tggggcggcc tgcccctcac cctgttgatc accgccaccg gactggccgg
    12181 tgctctgccc ctcggcatcg ccatcgccta cggccggcgc gcccgcctgc ccgtgctgcg
    12241 gggcctgctg accgcagccg tcgagctggc gcggggcatc ccgctggtgg ccgtgctgtt
    12301 catggcgtcc ttcctgctgc ccctgctgct gcccgaaggc accggaccgg gcatgctcgg
    12361 gcgggtgctg atcgccatcg tcctggtggc cgccgcctac ctgtcggaga tcgtgcgcgg
    12421 tgggctgcaa gccctcccca ccgggcagcg ggacgccgca acggccctcg gcctgacgcc
    12481 ctggcagatc gaacgcagca tcgtcctgcc ccaggtcctg cgcgccgtag cgccctccct
    12541 cgcgaacagc gccatcgccc tcttcaagga cagcgcgctg gtcacggtgg tgagtctcta
    12601 cgaactcacc ggcgccctcg ggctggccct ggccggtgat ccccaatggc gggcctacca
    12661 gctggaggcc tatatcttcc tggcggcgat ctactgggcc ggctgcgcgc tgctggcgcg
    12721 cctggcggtc cgtattgggg caccgcccga agcgggtccg ggtccgggtc cgcgtccctg
    12781 agcccggctc acgcgtccgg cagatccaga cgattgcggt agccgtagcg cgccagcatg
    12841 cggtaggcgg tgcgctcgga gacgcccagc acctccgcca ccttgcggcg attgccgtcg
    12901 tagcgctgga gcagatccgc caggtaatga cgttcgatct cctccaggct gggctcgccc
    12961 tggatgcgga tctgcccgtc cgcaccgtcg tcccccagac cctgcagggg cggcaggtga
    13021 cccggttcga cgacgccggt acggctggcg aggatgaggg cccgctccac cacgttgcgc
    13081 agctcacgca cattgccggg ccagtcgtag gccagcaggg cccccagcgc ctcgggcgag
    13141 aacaccggca ccggcccgtt gccggcacgc cgccgggcga aatgggcgac cagcgcggca
    13201 atgtcctcgc gccgctcgcg caggggcggc acggtgacct cgaaggcgct gatgcggtag
    13261 tagagatccg cgcggaactt gccttcgcgc accatgcgcg ccagatcccg gttggtggcg
    13321 agcaccaccc gcacgttggc gcgcaggtcg gtggtggcgc ccacccgccg gaagcgcccg
    13381 gtctcgagca cgcgcaggag cttggcctgc acgacggggc cgatctcgcc gatctcgtcg
    13441 aggaacaggg tgccctccgc agcggcctcg atgaggcccg gcttgcgccg gtcggcgccg
    13501 gtgaaggcgc cccgctcgtg gccgaagagc tctgactcga acagcgtctc ctgcagggtg
    13561 cagcaatcca ccgcgacgaa atgcccgtcg cgccgcccgc cggccgcgtg gatcgcctgg
    13621 gccaccagct ccttgccggc accgctctcg ccacggatca tcaccgtcac atcgtcgtcc
    13681 gcaacggcct ggacgagctc ccacagccga cgcatggccg ggctgtcgcc caccatcggg
    13741 ctgaacgccg ggccggtgct gcgggccgtg cgcagggcat cgccggcgcg caggcgggag
    13801 ccttccaggg cgcggtggat gaccagctcc agctcgtcca gattgaccgg cttgaccagg
    13861 taatccactg cgcccaggcg catggcctcc acggcctgat tgatggaacc gaaggccgtg
    13921 agcatgatca ccgggcgggt ctgggcgacc tccgccagcg ggccgaagtc ccgcgcatcg
    13981 ggcagattca tgtccagcag caccagctcc ggcgcgtcgc tctcgaggca aaggcgggcc
    14041 tcggaccagg cgcgcgcggc ggacacgtcg aaacccgcac gggacagggt cttgacgagc
    14101 aggtggttga ggacggggtc gtcttcgacc accaggatgg agcgcttcat ggggattctc
    14161 cgtcggcggc gggcaggcgc acatggaagg tgctgccgac gcccggcgtg ctatcaacgc
    14221 cgatacggcc accgtgccgt tcgacgatgg ccttgcagat ggcgagaccg agcccggagc
    14281 cctgctggcc gtcggcccgg cgactgtaga agggcatgaa caccagggcc tggtcatcgg
    14341 caccgatgcc cacaccggta tcgctcacgc tcaggtgata gtcggcacct tcccgaccca
    14401 gggcgacccg cagctgaccg ccgcggggca tggcgtggaa ggcgttctgg accagattga
    14461 ggatcacctg gcgcagctcg gcctcatcgg ccatcacgcg cgccccgggg ggttcgaggc
    14521 agatctgcac gctcacgccg gtcgtctggc gttcggcttc gagcagggcc agggtgtcct
    14581 ccacggcggc acgcacgtcc accggacgcg tctcgccggg cgggtggctg agggtcagga
    14641 ggcgctgggt cgaggcgacg cagcggtcga tctccttgtc caccagaacc aggtattcgg
    14701 tgacgtcctc gccgctcatc gtgccgtccc gcaggccgcg caggctggac tggagcgcca
    14761 gacggatgga cgccagcggg ttgtggatct cgtgggcgac gccgttggcg agcagcccca
    14821 cggccgacag gcgctgctcc tgggagaagc gcacctgatc gtcaagggag cggatcgcct
    14881 cgacgaccat gcggcggccg tccggggtgt gcacggcggc cgcgtggatc tcgacgctca
    14941 cctcgctgcc gtcacgccga cgcaggctca tcaccgtgcg caggggctcc ggccgcgccc
    15001 gcagttcggc cagcggacag cgcaccaggg tggtcggaca gggttcggac agaccgcggc
    15061 tgacccggtg gcagggctgg ccgatcgcct cgccggccgc gagcccgagc agacgcaggt
    15121 aggcgctgtt ggccagcacg atgcacccgt cttcggcgat gaccagcatg ggatcgggcg
    15181 ccgcgtccag cagactctgc aggtaggcct gctgacggcc cgaggcttcg atcatgcggc
    15241 ccagccgctc ggtcatggaa tcgaggcgca gccccaggcg gcccagctcg cccacgcccg
    15301 gttcgccggc gcgggcactc agatcgccct ccgcgacccg gtcggccacg tcccccaggc
    15361 gcgccaccgg caccagcacc tcgcgccgca cgacccagcc caccgccagg gcgaacagcc
    15421 cgagggcggc cagaccgagg ggcagaagcc ccctcatctg gccggagatg tcgccttcca
    15481 caaggccctt gaactccacc accagcaccc cgttcacggg atgcacgtcc acgggcccgt
    15541 ggcaggcggc gcagcgggcc tggttacgga tcgggtaggc aatgcgcagg cggtcatccg
    15601 cctccagcgg ttggatacgc ggcggcgatt cggcacagcc gctgcgcagg cacaggccgg
    15661 ccaccagatc gggccgccgg gtgcccaggt cggtagcccg ggacgcgaac cggatctcgc
    15721 cgtcaggatg gagcagcgcc gccgaggcca ccccctgcat atgccccagg cgcgagagga
    15781 tctcggccag gccgcccaga tcgcggcgca gcatggccgt gtgcaggctt gcttcgaaga
    15841 gccgcgcaat gcgctcggcc gaggcctcct gctcgcgccg catcgcctcc cgcgacaccc
    15901 accagatccc ggccgacagc atgccggaca ccatcagcag ggcaagcatg aggaccaggg
    15961 tgaggcggcg ggacagggag caggcggcgc ggagggacac gggacggatg ggcgttggat
    16021 gggggtcgga tgaccgaatt ttcagtctac caccaggcgt ccggagcgcc ccacggtggc
    16081 cacggcgcag atcgggcaca ggtcggcatc ctcggacgca gtggcaccgc gccggatcgg
    16141 cgcgtgacac cgtcggcacg gcttgacctg gaagaagtgc tgcggcccgc cgggccacgc
    16201 ctcgtccacc gcagccggac gcagcgcatc gcggtcgcac acttcgacgc acagctggca
    16261 gccggtacag cgcgcggcgt cgaaacgcaa tgccgcctgc ccgccaccct ccagccagcc
    16321 aagggccagc tccgggcaca gggtcgcgca cacgccgcag ccgttgcagc gggcggggtc
    16381 gaggaccacg ctccacagcc cgggaccggg acccagccgg gccaggcgct cgagggcgtc
    16441 gcggcgccgg tccgagccct cggcgttgcc accggcgccc tccgccaggg ctgcgccggg
    16501 acgttcgaag aagcggcgga aaaaactgcg gcgggcgtct tcgggacgaa cgccgcgcac
    16561 cgcgtcttcc gtcccggcca gcacgaccgg cgccgcgccg cgagcttgca gggcccggtt
    16621 gagcgccgcg agacgctgtg tgaggggctg ccccccgccg cgcggacagg tgccacagtc
    16681 accggtccgc agttcgagcc ggcgaagtcc ccgcccgtgg gcctgcagga tgcgggcctc
    16741 gctgagccca tggacgcagg gcagcctgag cgcatccgcc tcaagcgttc cgtcgtccgc
    16801 acagcgctcg cagacgaggc acgcacaccc cgcgccatcg ggccggagcc tgggcggctg
    16861 cgccagttcc agcacgcccg caggacaggc cggcacgcac acgccacagc cgtcgcaggc
    16921 gtcgggctga aaggcgacct cccgccagtc cacggtccac gcaccgcgcg gacaggcatc
    16981 gacacacgcg gtacaccggg aggtggccga gcgggcgtgg acgcaggcgt cgccgtcgat
    17041 gttcagcatg acctcggccg cgcctcagcg accggtctcg agacgctcgc ggaccgcgcg
    17101 gaccgccgcc agggtcacgt ccgccagcgt ggcatagaag cgggtcgcgg ctcggctggc
    17161 cacccgctcg gcgaaatcgg gcagccagac cagcaggtgc gcgtcgagaa agtgcgccgc
    17221 cgcgtccgcc tgagcgctgc ccagcagatg ggcgacgaag gccagctcat tgctgaggtg
    17281 atcgtccggc agcagccgcc agttgcgggc cacgacgccg tgggcctggt agcgggcgcg
    17341 cacgtcgaag acgctgccct ggagcatcag gtggtcctcg tcccgccaca cggattcgca
    17401 gggcgaggcg cgcagggcgt gggtaaggta gatggcggcg aagtcggccg ccagctcgtc
    17461 ggccaatccc ggcgcatcgt ccgggcggac gccggcaacg tcggccacag ccgcggcaaa
    17521 gcgcaggagc gctggcgcgt cggccgggag cacgcccagt ccgcagggaa agccgccctc
    17581 gtgcagggcg gcgatcagcg ccggcgagcg ctcggcctcg tggaaccagg ccagggccgc
    17641 cagatcctcg gcggcgcaga gcaggccggc cggcacggcg attccggccg ccacgggcag
    17701 cacgtccgtg gcactcattc ttcgacccgt cccttgggat ggggaatgaa gacgatggac
    17761 ggattggtca gctcgatgct ggccatgtgc ttgacctgac gcaccggctc ctcgcccggc
    17821 gccatggcct tggtggcgta ggtgccggcg cgcagctggt cgatgggccc cacgtcgagc
    17881 acgcgcatca tgcaggccat gacgcagtag ggcttgcgcc cggcgtcgat ctcatccacg
    17941 cacatattgc acttcttgac caggttatcc accgggtcga actggggcgc accgtagggg
    18001 caggaggcct cgcagcggcg acagccgatg cacagggtgg agtcgatgtc cacgatgccg
    18061 gtatcggcgc gcttccagat ggccccggtg ggacaggtgg gcaggcaggc cggttcggcg
    18121 cagtggttgc aggacatgtt caccttgtag gcgaagacat ccgggaactg gccgccttcc
    18181 acgtacatca cgcggcggaa gcgcggcccg gggccgaggc cgttcttgtc cttgcaggcg
    18241 atctcgcagg cccggcagcc gatgcagtcc atgttgttgt ggatgaaacc gagctgcctg
    18301 gcgggcttga ccggctcgta ggtggccgcg tcgcggcgcg cctgggcggc cggcacctcg
    18361 gcgcgggcct cggcggagag cttgcggatg cttttcttgt cagccatgga atgcgccccc
    18421 ttgctcagta gtcacgccgg aacaccgtgg accgcgctgc gcccagctgg tcccagccgg
    18481 gacgatgggc cagttcggtc ttgcggatgt tcaccagaac ggtgttgtag gcgaaggcac
    18541 cggccgggct cggctcgtcc agggtgagga agtccgggtt accggcccgg tcgacgccgt
    18601 cccggtccag gtccatccag gccccctcga agagcaccgc gacacccggc aggcagcgtt
    18661 cggtgacgta caccggcacc acgcaggtgc cccgctcgtt ccacacctcc accgtgtcgc
    18721 cggtctggat gccgagacgc cgggcgtcgg aggcgttgac ggtcacttcc tggtcgtagg
    18781 tttcccgcag ccacgggatg ttgtggaaga tggagtgggt gcgccagcgc gggtgcgggc
    18841 tgatcaggtg gaaggggaag tccttggtct tcgggctgtt gagggactcg aaaggctcga
    18901 tccagcgggg aatcggcgga atcggcgcgc cgtactgggt gagcgtccag tcggtgatac
    18961 cggccaggta gggggagaag atctcgatct tgcccgatcc ggtggggaac ggttcgccct
    19021 gctccacctg ggcacggaag gccaccagcg gctccttgaa ggtgaacttg tagacgccac
    19081 gcttcttgaa ttcgtcccag ctcatggtga cgccctggtg cgcctgcacc ttgcccttcc
    19141 accaggcgac gaggtaggcc tcatccactg cgtcggggtt gcggaagtag tcgcgggtgg
    19201 cacgcgggtt gtagcgcttg ccgaagttct tgacggtggg atccagcgct tcgatccgat
    19261 aggccagctc ggtgaagatc tggaagtcgg acttgctctc gcccaggggc tggatcacct
    19321 tggggcggtg gatgtagtag tggcccttgt accagggcag cgccacgtcg tgccgctcga
    19381 agtgggtggc caccgggaag agcacgtcgg cccagatgcc tgaaggggtg atggtggaat
    19441 ccaggcacac cacgagatcc agcttctcga tggcctggat ctccttgttg atgttggtga
    19501 gctggttgaa ccagtcggag ccctgccaga agatgccgcg gatgttggga atctggccgt
    19561 cgagttcatc ctcccgcggc cacagcccca cgtcctcgcg cttcagcttg gggtagttga
    19621 tcacgcagtg ggcccagcgg tccgacttga tggaggcgta ccagacgttg gcgtattcgt
    19681 cgtagggata ggccaccgac tccgaatgcc aggccttgcc cacgccctcg gcgcagccgc
    19741 ccagcttgcc gatgttgccg gtcatggcct gcactgcggc ggccatgcgg ttgtactgtt
    19801 cgccgtaggc ggcccggccg ggcgcccagg acgccttgag ggcggccggc ttggtggtgg
    19861 cgtacaggcg cgccagcttg cggatgtcgt cggccgacac gccgcagatg gcctcggccc
    19921 actccggcga cttgggcgtg ccgtcgcggg tgccgaggat gtagtccttg aagctctccg
    19981 cgtcggcgta gtccttgggc atcgtgccgg catccatgcc cagcacgaat ttgtcgatga
    20041 aggcctggtc gtggaggttc tcggtgaaga tcacgtgggc catgccggcc agcatggcgg
    20101 cgtcggtatt gggccggatc gggatccacc aggcgtcgta ggcggacgcc gagtcggtgt
    20161 attgcgggtc caccagcacg aacttgcagc cgcgctgctt ggccatgcgc atgtagtaga
    20221 aggtgttgcc gccgtggaag gtgtaggccg ggttccagcc ccacatgatg atgagcttgc
    20281 tgtgggcgta ggcgtcgtcc tcgttgccgt cctcgatggt gccgaaggtg atgcggctgg
    20341 agaaggtggt gccctggtag ctcggcaccg accaggaatt ggtgcgacag ccgaacatgc
    20401 ccaggaagcg cccgaggagg ccttcgatct ggtcggactt gtggagcacg ccgtaggagg
    20461 cgcccgcgta gctctggtcg agcagcgccg tggggccgta ggtcttcttc agctcgacca
    20521 tcttgcgggc gacgaaatcc agggcttcgt cccaggacac ccgcttgaac ttgccctcgc
    20581 cgcgcttgcc gacgcgcatc atgggataga gcaggcgctc cggcgaatac aggcgcgaac
    20641 ggtaggagcg gccccgcagg caggcgcgca gctggggctc atccaccgtg tccttgccga
    20701 agaagccgcc cttctggtaa cggccatcgt cggaggacag gcgcacgatc acgtcgccct
    20761 tcttgtgggc caccagcatg tggcgtgacc cgcagttatg ggcgcaggag gtgaccaccg
    20821 tagtgagctg gtcgtcggtg taatacggtt cgtaggcgaa ggccttggcg tctttcggcg
    20881 tcatcgtcag cccgcccagg ccggcgagtc cggccccgac gcccatcacc ttcaggaaat
    20941 cccgtcgggt cgtctccgtc gcagacggtg tgacgcccgc atcgagcacg ctgttcatgg
    21001 ttgttgtcct ttttcgctgg cacccgtcac gaagcgggct tcggaacggg tcggccgccc
    21061 cttcgcccag tctggcgtct cctccgccat gcccggccag ggatgacgcg gataggggta
    21121 ggtgatgcgg taccacgccc ggccgccgtg gcagccgtag caggcgtcac cgctctcctt
    21181 cccggccgtc tcgatggctt cgggcttcag atagttcacc tggtgccgct gggtccggat
    21241 gccctcgtgg cacagcaggc agttgttctg gaacatctcg cgactctcct tccacggccc
    21301 cggcagcccc atgacctgcc agttcatctg gccatgacac ttcaggcagg tggtctcggg
    21361 catcaccgtc ttgcgcatgc ggaaaccacc atcgcgcggg gaggtggcgg agctgagggg
    21421 atgtttgtcg cgggggtcga caccgtcgtg acaggtgttg cactggaggt tcatcaggct
    21481 tttcccgagc ccggtatcca ggtgacgacg atggaaatcg gcctgcgcac cctggtaggt
    21541 ggacagctgc tggtaccacg ccttggccga ggccgcggtg acacccgccg gcgagcgttc
    21601 ccgcggcctg cgggtcagga tctcgctgtg acaggcgaga cattgttcat tgctggcctg
    21661 ctggatcgcg ggccggaagt ggagcgggga ataacgggca cgctgataat cgaccccggt
    21721 cgcatcgtga ccttcggcgg cgagggccgg acccagggtc caggccgcgg ccagcgttgc
    21781 cagcaggcgc aggtactgct tgattcttct tgtgaccacc atggcagcct cgtgcattgc
    21841 gtccggccac acggccggca aaaaggatcc cggccgaagc cgggatccgg aagaaggctt
    21901 acttgccttc catgcccgac atgccggaca tgccgctcat gccttgctgc ttgtgcttct
    21961 tgtgctgatt gccggacatg ccgctcatac cagacatgcc ggacatgcct ttctggcctt
    22021 ccatgcccga catcccggac atgcccttct ggccctgcat gccggacatg ccgctcatgc
    22081 ccttctggcc ttccataccc gacatcccgg acatgccctt ctgaccctgc atgcctgaca
    22141 taccggacat gccgctcatg cctttctggc cttccatgcc ggacataccc gacatcccct
    22201 tctggccttc catgccggac atgccggaca tgccgctcat gcccttctga ccttccatac
    22261 cggacatgcc cgacatgccg gacatcccct tctgctcatc cttggccaca gcgggcagcg
    22321 cagcacagag agccagggcg agggcggaac agctgacgat cttgccgaaa gacttcatag
    22381 gtttctcctt ggtttgacag acttggactt ggaaaccggc ctcccccctg gcgggaaagt
    22441 caccggacag aacaagggcg gtgcgacccg ccccgaaaaa ctgggggatc actcccgcga
    22501 cccgtgccat cgacgcaggt catacggtcc ggtcatgccc ggccggaaca tgggcgagta
    22561 ccacgccccc tgctgcgcga tcacctccac gttcccgggc aaaggcaggc tgatgcccgt
    22621 gaggcggacg gccttcatct cgggcgtgag tacgaaggtc gtgatgtgcg gcgcacccgc
    22681 gggtcgccga tccggctgca ggccggccac aaacgcgccg gcggacacgg gcgcagacaa
    22741 caccaccgac atcgccacgg cactcaaaac catcagggaa cggatcatgg actgcacctc
    22801 cttgcagtcc ttatcgcaac ccgcgtgccc gcccgagcag gtactccaag cgactgtttt
    22861 aacggtggaa tttcattcac ctcagaccac gccggggttt cggcctgtca ccctgacaga
    22921 ttgacatgcc actctgacag tccgcacgcc cctcgcccca cgccggcgac gacgacgtgc
    22981 gccgcggtgg gcagccactc cggtccgtca aactcggcct agcaaccgtg cggcgaaacc
    23041 gctccaggcc ggaacgcacg gccccgctgc acatctgccg gattcagacg gcgtcgccgt
    23101 catcacgcgc acctcgcggc ctcgtccccg cgggcgcagg gtcgccacgc tgtcgatccg
    23161 gaacgggtca tcggcacctc gtgcgccgcg tcctgcccgc gcaccgcccc cgcatgtcct
    23221 ggcgcgtcgc ccgattcacg ggcatcagcc tgcagcggga cgggcgccca tcgccggagg
    23281 tacgtgcatc ggaatgcgca aaaagacagg gcccggaaac aaggtttccg ggccctgcat
    23341 ccttccgcgc cggccagtcc ggcgcgggaa ggcttcgtcg acttacttgg cgggagccgc
    23401 ttcggcagcc ggcgccgccg gggccgctgc cttgtgatga tggtggtgct tcttcgcctt
    23461 cggcttggcg gcgggttcag ccgccggggc ggtcgccgca gccgcaggcg ctgctgccgg
    23521 cgcagcggcc ggtgcctcgg cgaaagaagc ggtcgcaaac gcggaagcaa tggccagggc
    23581 gatgaatttg ttcagcatgt tctgtttctc ctagatcgga tcacgatgtt gtggggttgg
    23641 cagaatcacc agcctcaagc agaacaacgc gccaccctgc cagagcgtga caggcaaagt
    23701 gtaagcaaac attccacccc gccgcatcca cgtcaccccg gcctgtgata gcggaactcc
    23761 tgggtgcgcc cgccgtaccc gtaggacgca gcgcgcacat cgtgcacatg gatgtagctc
    23821 tcctcatgca cgtcacccag cagtcccgca aaggcatcga aggcctcgcg aatgtagcgg
    23881 gccttttcgt ccttggtatt ggtctcgtcg gtgatgcgga tgtcgaagaa gaagctgtgc
    23941 cgccgctgct ccgccagcgt ccgcccccct acgatccagt cttccggcgc cacgtagtcg
    24001 atggtgatcg ccgtcacctc tggcttcttg tgaaggatgc ggctcgtcag gtcgagcagc
    24061 atcccggcaa tgcgatgggt catttccaga gacttcggag cactgatctt caactggatg
    24121 atgggcatac acgcctcctt tttgattagc aacacaacta tataagcgaa caaaagccgc
    24181 taccccagcg caccccgcac gctccgcacc tgggcgagcg catcgacaaa caccgcctcg
    24241 ccaagcaccg tcttcagacg ggtggtgacc gcgccggccg cctcgttcac cgccggaccg
    24301 atccgttcgg cctccgccgt ggggaacacc gtcgcctccc gtccgtcccg cggcgacgac
    24361 cgacgctcca ccagcccctt ggccgccagc ccatccagcg cacgggtggc cgtcggacgg
    24421 gagattgcca ggaacgccgc cagttcaccg ggacaccggc cgggctcggc cacgaccgcc
    24481 cgcagcagga aggcctgggg cggcgtcagg ccgaagggac ggaaggcgtc ggcccattcc
    24541 ctttccaggc ggcgcgccag ggctgtcgtg ttgaaataca ggcattgatc gaacatggct
    24601 gcatggtagt acgatttaat tcgctatgca atcatattcc ggtggagtcc atcctctggg
    24661 gggccggtac atcccccgcc accttggcag ggcgaagcct gccgaactaa gatgggacat
    24721 gccatccgat ccgcatggat ccccttcctg accccgaaag cccccaactt gaaactgcac
    24781 gccttcgtcg ccatgccctt tggcaccaaa ccggatgctg ccggcaatcc cgttgacttc
    24841 aacgccgtct acgacgacct gatccgccct gccctcgaga aggccgggct ggacgtgttc
    24901 cgcgccgatg aagagccgcg cgccggcgac atccgcgccg acatgttcca ggaactcctg
    24961 atggccgacc tggtgctggc cgacctgagc atcgacaatc ccaacgtctg gtatgaactg
    25021 ggcgtgcgcc acgccctgcg cgcccgcggc gtgatcctcc tgcaggcccc ccgcgaccgt
    25081 cagcccttcg acatctacac cgaccgcaag ctccactacc gcctcaagga cggcaggcca
    25141 gacccggcga ccctggacga cgaccgcaag gccatcaccg acatggccac cgccaccctc
    25201 aaggcctggc acggacgcaa ggtcagcccg gtgtacgccc ttctccccaa tctgcaggag
    25261 cccgactgga agagcctcag gatcggcgac gcccgcgagt tctgggaacg ccacgacgcc
    25321 tggtgcgcgc tcatcgagcg ggcccgcgac gacaaccgtc cggaggacat cctcgtcctc
    25381 gccgatgaag cgcccatcac cgccttgcgt gtggaggccc gcctcgcggc cggcaacgcc
    25441 ctgctccagg gccggcactt caatttcgcc ctggaacaat tcgacctcgc ccaccgtttc
    25501 gacccggaca acctcaccgt cagccgcaag cggggcgtct gcctgcagcg caccggacgc
    25561 gccgccgagg cccaggatct gtaccgggac atcctggccg cccacccgga cgacgtggag
    25621 acctggtccc tcctgggccg cgtggacaag gacgcctggg tcaccgcctg gcggacgccc
    25681 ggcagcacac ccgaagccat gcgcgacgcg gccacctacg aggacgccct gctgcgcgcc
    25741 gccatcgaca gctacgccgc cgccttccgc gccgtgcccg gtcacttcta ctcaggcatc
    25801 aacgccgtca ccctgatggc cctctaccag gatctgaccg gcgacacccg ctacgccgcc
    25861 gaccaggccg tgatggccgg cgggatcgcc tggtcggccc attccaccct cacggcccgg
    25921 ccgtgcggcc aggcgccaca ggatctctac tgggcggccg ccaccctggg cgacctggcc
    25981 gtcctgcgcg gcgacgcccc ggcggtccgc gccgcttaca aggaagccat cgtccacgcc
    26041 gacaacgact ggttcgccct cgactccacc ctcggccagc tccgcctgct ggcggaactg
    26101 ggcttccggc ccgacaacgt ggccgccggc atcgccacct acgagcgtgc ccttgcccgc
    26161 ctgcgccccc cggagaagag ctggcagccc ggcaaggtct tccttttctc cggtcacatg
    26221 gtcgatgcgc cgggccgcaa ggaaccccgc ttcccggccg acaaggagcc cgtcgccgcc
    26281 gccgccatcg accaggcgct gagcgacgag ggcgccagcg atcaggacct cgccctcacc
    26341 cagggcgccg caggcggcga cctgctcttc gcggaagccg ccctgaaacg cggcctgcgc
    26401 ctccagctcc tgctccccct gcccgagccg gacttcatcg aagcctccat cctccgctcc
    26461 gccgacggcg agacctggcg caagcgttac ttccaggtcc gcgaccatgc cctgtgcctg
    26521 ccgccccgcg tgatgcccga cgtcctcggc cccctacccg tcgaccatct gggcaattcg
    26581 atgaacgaat tcgagcgctg taaccagtgg ctgctctaca gcgccctcgc ctgcggcatc
    26641 gcccgcaccc gcttcgtctg cctgtggaac ggcaccggcg gcgacggacc cggcggcacc
    26701 cagcacatgg tccaggaagt ccgtcggcgg accggcaagg tcacctggct cgatacacgc
    26761 aagctgtggt gatgccgcca ccggctcgat gaccatgctg cgtccgctgc ccctcctgtc
    26821 cctcgctgcg ctgttgctct tcgtgggcgt actgtggtcg gaccagcccc tggtcgaagc
    26881 cgggcacagc atggtcgaac tgcagttcgc gggatcggcc gagcgggccg cccggattct
    26941 cgacacctgg cggaccgcgg gcgtggagga cgcgatccgc ctcaaccagc agatcgactt
    27001 cgccttcctg ctgacctacc cggcggccct gtccctcgcc tgccgcagcc tcgccggaca
    27061 tctcggtccc ggccagcgca tcggccactt cctgacccgc gcagtctttg cctgcacggt
    27121 gctggacggt gcagagaatc tcgccatcca ggtcatgctc gaccacggcg cccgcgacag
    27181 catggcccgc cttgccacag cctgcgcgat accaaagttt gccctcgccg cctgggccat
    27241 tggctggtgc ctgtgggcgc tgttgcgccg cgtccgactg ccatgagcac tcaggcgcac
    27301 ggtcacccgc acggaccacg ccagaggcgc tctttcgccg cccgcctgtt cgggtacgac
    27361 ctgttcatct ccttcgccct cggccatccg ccccgcggca cccagtccta tgcctcggat
    27421 ctggcacgcc gcctgcggga gcgggacttt gccgtgttct acagcgaaga cgaggcggcc
    27481 cccggcggcg aactggactc gaccctgcgc aacgccctcg cccgcgcgcg catcctggtg
    27541 gtcatcgcca accgcggcac cctcgccgac ccccgctggg tgcgccgtga agtggaggag
    27601 ttccgcgcac tccatcccgg ccgccccatc atcccgatca gcctcgatgg cgccctgcag
    27661 gatccgcaac tggccgctgc ctgcaaggac tggctggaca tgagcgacac catctggctc
    27721 gacgacaccg cagaggccgc cgagcacgga ctggtgagcc cggccgtcat cgaccggctt
    27781 gtcctcgcgc ccacccagat gcgctcgtcc cagggctggc gcatcacggt cgccgccgtc
    27841 agcctgacgc tgatcggact caccgcatcg tccatctggt ccgaacatcg cgcccgcacc
    27901 aatgaaaact ccgcccgcca ggaagccgag cacgcacgcc gcgccgaacg ccaggcgcgc
    27961 gaagagctgc gcgtctcgac gggcctgcgc ctgcgcgccg aatcatccgc catgctcggc
    28021 ggcctacgcc cggagccaga cgaacgggcc ctcctgcaac tgctcgccgc ccttcatctg
    28081 ggacgcggcg acgacaccct cgacccggat gcggaaggcg agctcctcgg gggcctgcgc
    28141 cacggtcgca acctcgtccg catgtcgccc gccgcccacc ccatcctgag ccttgccctc
    28201 agtcgcgacg ggacccacct cgtcaccggc ggcgaagatg ccaccttgca gctgtggaac
    28261 gcgctgaccg gcgaccccgt cggcccaccg ctgcgcgccc acgaagacgc cgtgcgggcc
    28321 gtggatttca gcccggacgg gcagtggctg gtcagtgcca gcaccgacaa caccctgcgt
    28381 ctgtggcagg tgggtcgcga cggcctgcag ccggctccgc ttccgcccct gaaaggccac
    28441 accgactccg tgcggaccgt ggccttcagc ccggacggtc gcctcattgc ctcgggcagc
    28501 gacgacaaca ccgtcatcct gtgggacgcc cgcagcggca cgcccctgcc ccgacgcctg
    28561 accggccacg gcgattccgt caccggcctc gcgttcctgc cggacgggcg gcggctcgtc
    28621 accgccagca acgacatgag cctgcgcctg tgggacattc ccagcggccg ccccctgggc
    28681 ccgcccctgt ccggccacac ggactccgtc aaatgcgtgg ccgtctcccc ggacgggcga
    28741 cgcatcgtgt ccggcgcctg ggacaacacg ctccggctgt gggatccggt gacccgccgc
    28801 cccatcggcc gccccctcac cggccaccgt gccctggttc gcagcgtggc cttcagcgcc
    28861 gacggtcggc gcatcgtgtc gagcagcgac gacggcaccc tgcgcttgtg ggacgcccgc
    28921 cgcggaacgc tcctcgcgcc gcccttgcgg ggccaccagg gcgaagtgac cagcgccctg
    28981 ttcgcccgca aggggcagcg gatcatctcc ggcggcatgg accggagtct gcgccagtgg
    29041 gatccgcgca ccacctcgcc cctcggcgaa gcacagtctg ccggatccgc cgcgctcctc
    29101 agcgtggccg tcagcccgga cggcaaacgc ctggctggca gcggcgaaga cctccgcggc
    29161 cgcctgtggc gggtcgcctc tgcacgcacc gcggcaaacg cccgcgggat cgccctgtca
    29221 ggcccctccg ccgcgttcta tgccctcgag ttcaacgccg acggcgcccg cctggcgggg
    29281 ggcgacgccg ccggccagct gtggcaatgg gacggacgca gcggcgaaac cctcgccgca
    29341 ccccaccccg cccacgcgcg ccccatctcc gcgctcgcct acagcccgga cagccgccgc
    29401 atcgccaccg gtagctggga caacacgatc cgcatctggg acgccaccag cggcgccccg
    29461 ctcgggccgc ccctcaccgg ccatcgcggc tggatcgccg cccttgcctt cagtccggac
    29521 ggccggaccc tggtctccgc ctcggccgac acgaccctgc gccgctggga cgtggccacc
    29581 ggccatcccc tcggggcgcc cctgtccggc cacaccaact gggtgctggc ggtggctttc
    29641 agcccggacg gccggaacct cgcctcggcg ggcgcggacc gtcagatccg ccgctggaac
    29701 gccgtcaccg gcgcaggcat cggcaccccg tccggcggcc acgaatcctg gatcaccagc
    29761 ctcgccttca gtccggacag ccgctatctc gtctccgcag gctgggatcg cagtctgcgt
    29821 ctgtggcgca ctgacagcgg actccccgtg ggcatcccgc tgcaagggca ccgggactgg
    29881 atccagaagg tcgcattccg cgtagacgga ttgcgcatcc actctgccga ccgggccggc
    29941 gccctgcacc tctgggctgc accacgccac tggccggaga tgctgtgcgc caaactcagc
    30001 cgcaacctga gccgcgccca gtggaaggcc tgggtctcgc cggacatcga ctacgtggtc
    30061 cagtgtcccg gcctgcccgt gccgcccgac tgacgcgtcc ccctggagac cgccatgcac
    30121 gaacccgccg acatccaccg cctgttcgcc gaacgcttcg ccgcggccga tctggacggc
    30181 ctcctcgaac tctacgcgcc agacgcgctg ctgctgcccc aggcgggcag cgaggtgcgc
    30241 ggccacgccg ccatccgcga agccctcggc ggcttcatcg ccctcggcgg acgcttcacg
    30301 atggcgccgc cccgctgcct gcgggcgggc gatatcgccg tcctgcacgc cgactggcag
    30361 ctctccgcca gcggcccgga cggacagccc gtccacctgg ggggacacac cgccgacgtc
    30421 gtgcagcgtc aggtcgacgg cacctggcgc ctcatcatcg acaatccatg gggcgacgcg
    30481 gtgaccgcct gaagccggcg ccccggccgg ccggtgtaaa ggagaccgga tatcgcatgc
    30541 ggatttgcga aacggccggc gcctgcatat agtccttgaa gaaccccgca cccgcgccct
    30601 tcaggagacc gtcatgaccc agacccttgc cgaccggatc cgcgcccccg gtccgaagaa
    30661 aatcctgtcc tgtgacggcg ggggcatcct cggcctgatc agcgtcgaga tcctcgccaa
    30721 actcgaagcc gatctgcgcg tccgcaccgg caaacccgat cttctgctgg gccaatggtt
    30781 cgatttcgtt tgcggcacca gcaccggcgc catcatcgcc gcctgcatcg ccgccggcat
    30841 gagcatggag cgcatccgga cgttctacat cgaaagcggc cagcagatgt tcgacaaggc
    30901 cagcctcttc cgccgcctgc aatacagcta caacgacgag cccctcgccc gcaagctgcg
    30961 cgacgaattc acccgcgccc tgggcgacga caccaccctg ggcagcccgg gtctgcgtcc
    31021 gctgctcatg atgatgatgc gcaacgccac caccgattcg ccgtggccgg tctccaacaa
    31081 ccccttcggc aagtacaacc agcccgcccg caaggactgc aacctgcgct tgcccctgtg
    31141 gcagctcgtg cgcgccagca ccgcggcccc cacctatttc ccgccggagg tcgtcacgct
    31201 cggccagggg gatgacgcct acagcttcat ctttgtcgat ggcggtgtca ccacctacaa
    31261 caacgccgcc ttcatggcct tccagatggc caccgccacc cccttcggca tccgctgggc
    31321 cacgggcacc cgcgagatgc tgatcgtctc ggtgggcacc ggcagcgtcg ccgccgcccg
    31381 gccggacctc gaggccgacg acctgaccct catcgaaaac gccaagaccc tgcccggtgc
    31441 cctcatgaac gcggccgccg cgggatggga ccgggcctgc cggctgatcg gcgagtgccg
    31501 cttcggcgcc ctgatcgaca gtgaattcgg cgatatggtc gtcccgcccg gcggcgccaa
    31561 cacctacagc ggcagcaagc agttcgccta cgtgcgctac gatccggacg tcagccaggc
    31621 cgggctcaat gcgctcggcc tctccggcat caaggccgcg gacgtgcaga ccctcgactc
    31681 cgtggcccac attcccgaca tccggacggt cggcaagacc tacgccgatc gcaacgtgaa
    31741 gctggaggaa cacctgcgcg gctttgtctg agggcccgat ccggcaactt ccgccgacag
    31801 accggcaaca tttgccgcat tcacgccacc ttgcgccgac cgggacgccg ccatgacgcc
    31861 caaaacctgc ttcgtcatcc agggcttcgg caagaagacc gactacaccg acggccgcgt
    31921 gctggacatg gacgcctcct atggcgtcat caaggacgcg gtcgaggccg ccggcctcgt
    31981 ctgtctgcgg gccgacgaga ttccccattc cggtaccatc gacgagccca tgtaccggca
    32041 gctcctgcag gccgatctgg tgatcgccga cctgtccacc tacaacgtca atgcagcctt
    32101 cgagctgggc gtgcgctacg gcctgcggcc ccgcgccacc ctcatcgttg ccgaagaggg
    32161 cttcaagaac cccttcgacg tgagccatct ggtgatccgc cgctacaagc acctgggcga
    32221 ggacatcggc gtcaaggagg cccgccgctt cgccgacgag ctcaagctgg ccatcgctgc
    32281 catcctgtct gcagaccagg cggacagccc ggtctacacc ttcctgcccg gcctgcagcc
    32341 ccctgcgctg ccacccccgg ccccacgcgc cgcccctgtc caggcccagg ccgaagcccc
    32401 cacccgcgcc ggcggcttcg atctcagcaa ggcgcgcgac tggctcgacg ggctcttcca
    32461 cacctcggcc cccgagccgg gcacggatgc cgccgcggac accgcgggac cgcccaccct
    32521 cagccgctgg gaacccccgg aagtacccgc tccggccgcc ccccgcagtg ccaaatccct
    32581 gctcgacgaa gccctcgccg ccctgcaatc cggggaggat acgccgcccg atttcatccg
    32641 cgcccgcagg ctgctggaag ccgtccgcga acagcgcccc aacgacccct tcgtgatcca
    32701 gcaactggtc cgggcgattt ccggcgccgc ccgtccaacg ccccaggcag ccctgcggga
    32761 ggccctcgcg ctgatgaacg agctcggccc cgccaccacc aacgacccgg agaccctcag
    32821 cctgtggggc agcgttcacc agcgccgatg ggaacttgat gcgcagccgg ccgatctgga
    32881 ggaagccatc gccggcttcg agcgagggta ttttgtgaaa caggactggc aaacaggcag
    32941 tgcgctggcc tttctgctcg accgccgcgc acttgagcat ctgaagtccg gcgcaagcga
    33001 cgaagcggtt gcggacggcg tgcaggcacg ccgccttcgg cgcgaggtgc tgcgccttgc
    33061 cgggccacac atcgaccacc ccgccgacga tgccgcaaag cggttcagcg tgatgtgcgc
    33121 cgtctgggaa gcccaccttg ggctgggcgc aaggtccgag gccgacatgc tcgacgcgcg
    33181 ccttgacgct ctgccggtac cggcacccct caacgcccgc cgaaaggcgc atgggcgcac
    33241 cctggtggca caactcgacg ccttcacgcg cttgcgcggt ggcgccgccg gatcgacgtg
    33301 acgggatccg tgtcccgcaa gaggccctga gcgtgcgacg gttgaccggc aggcgcactc
    33361 gccgtttccc ccgaacgacg cgcccccgcc gccgcgctgc cttcaacccc cgtaaccacc
    33421 catagatccg cacggcggac ctgccgatac tgattcgcgc ccgatgccgg catcgcacct
    33481 acaagcgcgt ccctcgcgcg agccgtcggc catgatgccc tgagggcgcg ggcgaacagg
    33541 gcgtcagccc tgctccggat cgccaccccg gcgctccttg ggcgcgccgc tgaagcggct
    33601 cggcggcacg ccgagcgcgc gccggaacat ggcggagaac gctgcctgac tcgcgtaccc
    33661 tacctccgcc gccacacggg ccagcggcac cccgcgcgtg atgtcatcca cggcgcgggc
    33721 caagcgcagc tgctgacgcc aggcaccgaa gctggtatcc agctcggcgc ggaacagacg
    33781 cgccagggtc cgttcgctgg ccgccacctc ccgcgcccac tcggccagtc cccggtcatc
    33841 ggccgggtcg gccatcaggc ggtcgcacaa ctgcttgagg cgccggtcct tgggcagggg
    33901 caggcccagg gacaagggtg gcgcgacgcg gatctcctcc aggaccagtt ccatcatcaa
    33961 acgatgacgc cggtcggtgc gccgctcggc cagggcctcg atcaggttgc gcaacagcgg
    34021 cgacacatcc accacagcac agcggtccag ggccagcggc gccacttccc gggcgatgta
    34081 gagcgtgcgc agatccacct gaccgatcat caccacgtcg tgctccatct ccggcggaat
    34141 ccacactgcc cgaaacggcg gcacgatcca cgcaatgccg gcggccgcaa tgcgcagcgc
    34201 gccggacacc ggataggcca gttgccccca cgggtggcga tggggcggca ctgccacgtc
    34261 gggcggcaca cgacgctggt tgacggtcac cggctcgtca gcggacgggg ccaggcgcgg
    34321 gagacaatcg aaggcgacac tgacgacctc agccggcatg atttgtctcc tttgaacaag
    34381 ctaatgacgg tatatcgaat aacggccact gctcgaaccg ataccatggg cgtcgatgtg
    34441 cggaaacacg ccccagagcc tgccgccccc ccgaccccag tccggagaac agccatgtca
    34501 agcgatgccg cgctgtccgc gccgccccgc cacgacgcct ttgtgatcgc cctcatcggg
    34561 tcggtacacg gtgtctcgca tttcttccat ctgctgctgc cacccctttt cccctggctg
    34621 atgcccgaat tcggcctgga ctattcgcgt atcggaacga ccatgacggt cttcttcgtg
    34681 gtttccggcg tgggtcaggc cctggccggt ctggcggtgg atcgcttcgg tcccttgcgg
    34741 gtgcttgcct cgggcatcgg atgttttgtg cttgcgggtc tgaccctttc ccgggcaaac
    34801 ggtttcgctg atctgatgat cgtggcggcc ctggctggtc tcggcaacag cgtcttccat
    34861 ccctgcgact tcacggtcct caaccgtaac gtgtcggtgc cccgcctcgg ccacgccttt
    34921 tcggtgcacg gtctttccgg caatgtgggc tgggccctgg cgccgctctt catgacgggc
    34981 atcgccacgg ctgccggctg gcgcaccgcg gccctggccg ccggcctgct ggcgattccg
    35041 gcgctggccc tgctcctcgc cggccggcgt catctcgtgg acgccacgcc gccggagcac
    35101 caggacggtc gccgggcgag caccgctgaa atcctgcgcg tcggccccgt gtggctgtgc
    35161 tttgcgttct tcttctttct cacgacagcc ttcggcgcca tccaggcctt cggcacgccg
    35221 ctgatgcagc acctctatga gctgcccctg ggcatcgcag ccggtaccgt gtcgtcctac
    35281 ctgtttgccg gcgcggtggg gatcttcgtc ggcggcttcc tggcccgcag cgtgcaacag
    35341 gaccgggtga tcgccctggc cctgagcgcc gccgcggcca tggcggtgct gctcgccagt
    35401 gcgcaggtgc cggccggggc cgtgctgccg gtgatggcct gcatcggctt cctgagcggt
    35461 ctcgccggcc cgtcacggga tctgctggtg cgccgcgccg ccaccgcccg tttcggtcag
    35521 gcagccttcg ggcgggtgta tggctttgtc tactccggac tcgacacagg cctcgccctg
    35581 gcaccgctgg tattcggcgg cttcatggac agcggccact acggcaccgt gctggtgggc
    35641 atcgccctgc tccaggtggc ggcggtattc accgcactca aggtggggcg tggctgatgg
    35701 cgcggatctt cagctttccg ccggtggctg cgccggatgc gcgggtgctg atcctgggca
    35761 gcatgccggg cgaagcgtcc ctgaaggccc gccagtatta cgcccatcca cgcaacctgt
    35821 tctggcccat cctcggagaa ctggtgggcg cccgaccgga tctgccctac ccggacaggc
    35881 tcgccgcgct gcgtcaggcc cgcatcgccc tgtgggacgt actccagtcc tgcgagcggg
    35941 atggcagtct ggattcggcc atcgacccgc agacgcgcgt ggtgaacgac ttcggtgatt
    36001 tcttcacccg tcacccgcac atcggctgga tcttctttaa cggccgggaa gcggagaaga
    36061 ccttccgcaa gcacgccatc cacgtctgcg acgcggagcg cttccgttac accctcctgc
    36121 cctccacaag cccggccaac gcgtcgattc cgccggccgt gcgcctggac gcctggcgcg
    36181 tcatcgtgga tgcgcccagg cgggtgtcgg cgccgcatcc cggctcgatg gccggcgcgg
    36241 gtgcgacact tcagggttca agcacgtagg tccccggtgc atcggcgagg gccggatggg
    36301 cctcgtcggc caccgggcgg gcgtccacca gatcgccgca ctggggcttg agccacgcca
    36361 tccagtctgt ccaccagctg ccgggatgct cggccgcctt ggccagccag ccatctgctg
    36421 cgtcgctgcg ccgcgccggg cccacgtggt aacgacgctt gggcggattg accggcggat
    36481 tgacgatgcc caggatgtgc ccggaactgg acagcacata acgcttgggt cccgccacgt
    36541 agttcatggt gcggaaggtc tgctgccacg gtgcgatgtg gtcgtcttcg gcagcgactg
    36601 cgtagaccgg ctgtgcgatc gcacccaggt cgatgggttc gcccgccacc gtgagcgcgt
    36661 ccttctcgat cagccggttg tgcaggtaga gttcgcgcag ataccaggcg tgcattgcat
    36721 agggcatgcg cgtggaatcc atgttccagt acagcacatc gaagggcgcc ggcttctctc
    36781 cgtacagcca gccatgcacc acgtagtgcc aaatgaggct gttcgaccgc agcagacgaa
    36841 aggccgacgc catctcggca ccgtccagat agcccttctg ggccatggtc tgggtgaggt
    36901 agcggatgct gccctcatcc acgaagacct cgatgtcgcc gggcttgtgg aaatccacca
    36961 gggtcgtgaa gagggtgaag tgcgccaccg ggacgtcttc ggccttgaag tgacgattcg
    37021 cccaggccat gtaggtggtc agcacggtgc cgccgatgca gtagccgacc gcatgaacct
    37081 tgtccgcacc gctcatggca cgggcggtct ccaccagcat gtggacacct tcggtcaggt
    37141 aatcgtcgaa gcgcacgtcg cgcatgtcct cacccgggtt cttccagctc gtgatgaaca
    37201 cgtccagtcc ctggtcgagg agatagcgga tcatgctttt tcgggacgtg agatcaagga
    37261 tgtagaactt gttgatccac ggcgtgacga tcaccaccgg acgtgcgtgg atcttcggct
    37321 ggctcggggt gtagtggatc acctccagca ggcggttgcg gaataccacc ttgccgggtg
    37381 tcgtggccag gttgacgccc acctggaagt cattgggatc ggtcatgcgg acgttgcccg
    37441 cctccatatc ggccagaaag ttctggaagc ccttcaccag actctcacca cgggtctcca
    37501 tcgccagacg caaggcaacc gggttggtca tcaggaagtt ggtgggcgcc atggcgttga
    37561 gccacttccg ccaccagaac gcagcgcgcc gccgctcctt ctgggacagc cccggcgtgt
    37621 cgtagagcat gtcctgcatg tggtgcgtga acaccagata ccattccttg atcaggtccc
    37681 aggtcggcga ctccccccac accggatctg caaatcgcgt gtcatcacca tggggcttct
    37741 ccgggtcctc gctgggcagg ccaaagacac gacgcatcga gtgccactgc agtgcccaca
    37801 ggctggtgga caccttggcg agtgcatctg ccagctcctg gggatgagcc atccacgcca
    37861 actgcgcatg gacgatgggc gcggtcatgc cgatggggtc gatcgacgct tcaacgctgt
    37921 catgaatctt cttcagggac ttgcgggccg tactgaacgg agcggggggt tcacgcgatg
    37981 acatgttcaa agactcctgg cgacgtatgg tgttgacgca cgcacagggc aaatcttgat
    38041 cctgccggcg accgaggcgt tgatgtacgt caagccggtg gcgcgcacgc cctgcgcacg
    38101 ccgaccctgc ccgtataatg aaaacgtcag gttacaccga tgggagtgac gccatgttca
    38161 aacacattct tgttccaacc gacggctctg aactgtcctc ggccacggtg acgcgggccg
    38221 tgaccttcgc ccgggaagct ggtgcgaaga tcaccttttt ctatgcccag ccggatttcc
    38281 cgatgcccat ctacggcgaa ggtgcgctga tcgaccctac caccccggaa cagttcagca
    38341 aggcggcagc cgctgaagcc gacgccatac tcgcaaagtc ccgtgagctc gctacggtgg
    38401 caggtgtcga gtgcgccgga gtcacggtcg tcagcgaagt accgtacgaa gcaatcatcg
    38461 aggcggccga aaaaagcgct tgcgacctca tcttcatggc ctcccacggt cggcgaggct
    38521 tgtcgggttt tctgctgggc agcgagacac agaaagtgct gacccacagc aagatcccgg
    38581 tcttggtcta tcgctgaacc ggcgcccccg gtgccacggt cggcgcgcgt ccgtgcaccg
    38641 ggcccggcct cgttttgaaa gccttcacca ctgcggcaat accgctcggt caagggcttt
    38701 atccagaagc cggtgacgta aagggtggag cgtgctctgc cgagaggggc tttggcgccc
    38761 ccttcatttt ttcgacgcct acaaactgca tccgggtacg ccgtcgtaac aggtggacat
    38821 gtcgcttgcg ctcggtcata gcgacagacc agaccttgtc gtgctgcaga aggtacagcg
    38881 cgcgcatgaa gctcggggct accgcgttgg ccatcgccaa gcacatcagg ttgtaagtgc
    38941 gacgctgaca gccttcatcg ctttaatcag ctgagtaaaa atgaaaatgc cgttcagtga
    39001 gcactgaacg gcaataccgc ttaggcggga gtttattttt agcgcatagc ggaacagtcc
    39061 ggattccgat acgccaggcg gtttgtctaa acgccgtcga gtttgttgat ttctgccgac
    39121 gtgcgctgga aaaacagggt catcaggctg gacgcagcac agacaagcca gaagccgaaa
    39181 aaaccgatgc tataggtcgc cgtcgccgaa aagtgcaccg gctcaccgaa caggtacagc
    39241 tcctgtggat tgacgaccgt aaagaatacg atctcggcga gaccggccac cagaaaggac
    39301 ggccacagga cttcgatcag ctttctcatc tttcatgctc ctccatgccg agcgctctgc
    39361 tgaatggact gctcagcgga cagtcggctt aacccccgcg tgcgcggcgc tcatcttcag
    39421 ccatatgtga tttggcataa cgatacaggt agccgcccat cacgatgacg aacccgatgg
    39481 tgaacaagct caacatgcca atatcggtgc tgaacaattc tttccaagcc atgattttcc
    39541 tccatgtcaa tcgacgggtt tgaggtccgc cgaatccagg cggatctccg tctctgtcgg
    39601 gaggaacgcg gtccctgtca gcctccagct gcgggactca tcttctaaaa gtactttcca
    39661 gtgggcgctc acgagttcct gggcgagcgc gccccggtat gcccccgcct gcccctgcag
    39721 gttcaggtgt tggtccaggc ctccacgcgt cgggtgggca atcgtcacca gaacccgctc
    39781 gggaagggca atgccgggct ttgccgtcag cgcaatcgcc acaccgtcgg ccgagaacct
    39841 cacctgcgcg acaagcccca gatcgcgagc caccaccgca cgcccgatgg tctggttgat
    39901 cgccagaccc tgcttgtagt aatcgtcagc caccaatcca tcaaaagacg tgattgccag
    39961 ccacagggtg atgaacccag cgacgatcgc cgtcgctggc atcgccatca acaaccatgg
    40021 ccaacgctcc cgataccagg gcttgtcatc gcgaatcgta ttcagggtgg aagtattcat
    40081 tgcggtagca ggaatgcagt cttttcacga acggcgacgg tttggtcgtc cgccgctttc
    40141 acttcgatgt agatggtatt ggagccaggc ttaccggact ccggcggcgc agcaacctcg
    40201 accgtcacgg ccttcagacc ggcggcgtcg acttcgaagg gcttgtttgg cgaaatccgg
    40261 atctgatcca gaccgctcac ggacaaggta aaggtacggg gcttttcggt catattcatg
    40321 acctgcaggt tgtagacgtt agcgatcaga ccaccctcca cctctcggct gagggtcgag
    40381 cggtccttga tcacgtccac ccgcagcgtg gagcgggtcg ccagacccca gaccagtgca
    40441 atcaccagcg ccaccaggac cgacgaatag atgaggatgc gcggtcgcat cacgtggccg
    40501 aggatttcct tgcgacccca atgacgccgc atggcgttct cggtggtgta acggatgagc
    40561 cccttcggg
//
LOCUS       DAOQMM010000019        39716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_249_length_39716_cov_45.798391, whole genome shotgun sequence.
ACCESSION   DAOQMM010000019 DAOQMM010000000
VERSION     DAOQMM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 39716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39716
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249_length_39716_cov_45.798391"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLH85_05160"
     CDS             complement(<1..824)
                     /locus_tag="PLH85_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB63980.1"
                     /translation="MSTYVAPVKDMLFDMKELAGLDEIATLPGNEEVSQDLVEAILDE
                     AAKFASEVVDPLNRVGDKQGNKWDNGVVTTADGFKEAYATFCETGWNGMPASTEFGGQ
                     GLPVTVSTAVLEMWKSASISFSLCQMLTLGAVEAIAHHASDELKATYLPNMVSGKWTG
                     TMNLTEPQAGSDLAAIRSKADPRGDGSYAITGTKIFITWGEHDMAENIIHLVLARLPD
                     APPGVKGISLFIAPKFLVNADGSLGERNDLICSSIEHKLGIHGSATAVMSFGDKGGA"
     gene            complement(911..1846)
                     /locus_tag="PLH85_05165"
     CDS             complement(911..1846)
                     /locus_tag="PLH85_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB63981.1"
                     /translation="MRALVIAEHDHGSLKAATLNTVTAAAKLGAPVDVLVAGAACAAA
                     ADAATKLAGVSTVKVADAPAYADQGAENLAALLVDVIKTGGYSHVLAPATTLGKNVLP
                     RVAALLDVAQISDIVAIESADTFVRPIYAGNALATVKSADAIKVVTVRTTAFDAVEPG
                     GSATLEAVAAGPDLGLSSLVGRELTVSARPELGAAKIIVSGGRGLGSGDAYRSVLEPL
                     ADKLGAALGASRAAVDAGFVPNDYQVGQTGKIVAPQLYVAVGISGAIQHLAGMKDSKV
                     IVAINKDPEAPIFQVADYGLVADLFEAVPQLVAAL"
     gene            complement(1849..2598)
                     /locus_tag="PLH85_05170"
     CDS             complement(1849..2598)
                     /locus_tag="PLH85_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNB63982.1"
                     /translation="MKILVPVKRVVDYNVKIRVKADQSGVELANVKMAMNPFDEIAVE
                     EAVRLKEAQKATEVVAFSAGVAQCQETLRTAMAIGADRGILAETDADLQPLAVAKLLK
                     AVVAKEQPQLVILGKQAIDDDANQTGQMLAALLGWPQATFASKVELGADRVTVTREID
                     GGLETLSLPLPAVITTDLRLNEPRYATLPNIMKAKKKPLETVKPADLGVDVAPRLATL
                     KVVEPAARSAGVMVGDVAQLVNKLKNEAKVI"
     gene            3028..5070
                     /locus_tag="PLH85_05175"
     CDS             3028..5070
                     /locus_tag="PLH85_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HNB63983.1"
                     /translation="MNALDDSAAAGDAAQSGAEGDRRYREMLGAGLDLLDQGVTVFDA
                     DLRMVAWNAAFLRLLEFPAELARPGVSFDDFIRYNARRGEYGPGDPEAQVAERVTRAR
                     SFQAHTTERVRPNGQVLLIRGAPLPHRGFITLYTDITEERRAQAELEEHIRVRTEALT
                     RANAELTAAVESNKAIAAALQRSEQRLREITDAIPAAVAYFDQNWTYRYANKGYAEWF
                     GWSAERIEGRSIREVIGQEVFDIVSPYVTRALAGERMAYEYTLTGRDGGPAHARSTLV
                     PDFGADGQVIGCYVHSVDITEPRRTQMALSQAQKMEAIGQLTGGLAHDFNNMLTVVMG
                     NLAALRENHPQDPLTENFVEPAMQAAHRGAELIRRLLSFARKQPLEARPVEVNELILG
                     MSKLIRRSLPGTIAVSTESREPCLVALVDEHQLENALLNLALNARDAMPNGGELRIES
                     SLETLSPSAAADLEVPPGPYVQIAVTDNGMGMDGSTLARVFEPFFTTKQFGMGSGLGM
                     SMVYGFVKQSGGGVRIRSRQARGTTVALLLPWVEDTADSEVVPVGAPVSEADLAGKLV
                     LLAEDDADVRTVVRMQLAELGCAVVEAENGAEAADMVENIPAIALVISDVVMPGDMDG
                     RALARFVRRFRPELPVVLMSGFADSAAAFDADLALPLLAKPFTRDKLLAALGPLAA"
     gene            5084..5797
                     /locus_tag="PLH85_05180"
     CDS             5084..5797
                     /locus_tag="PLH85_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB63984.1"
                     /translation="MTRNPELIYVVEDELDVARLIRAALEKFGFRTEVFRTAEAFFHG
                     VRHRVPDLVILDLGLPDMDGMAVLQQLRSKHTCGLLVLTGRGDVYDRVMGLELGADDY
                     VVKPYEPREMIARVRSILRRYPRQPVVPADPTQRVAEFAGWRFETGSNTLVGPDGQQE
                     TLSTAEAQLLSVLLAHPNHILTREQLLGGRDVSALDRSIDLRISRLRRRFEENPQHAK
                     LIKTVYGAGYLLAATVTWS"
     gene            5890..6360
                     /locus_tag="PLH85_05185"
     CDS             5890..6360
                     /locus_tag="PLH85_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNB63985.1"
                     /translation="MRHARMLALLVGTMMMAGAAMAADVEVRDAWVRGVVPAQKSTGA
                     FMQLTSPAGATLVGAASPAAAKVELHEMAMEGNVMRMRPVPRLALAPGQTVALKPGGY
                     HVMLIGLKKPLVDGDKVPLTLLLEGRDKQVDKVEVSAEVRPLTAAAMDEHHHHH"
     gene            complement(6391..6768)
                     /locus_tag="PLH85_05190"
     CDS             complement(6391..6768)
                     /locus_tag="PLH85_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63986.1"
                     /translation="MSTEALAARLAAWAAVTPLTPSDVDCTTTVMLKILDGKCKMSPT
                     DKVQMAALYDLLKDRPGARFGADFHALIARAREGLDEALRMEVYEQRLLAETRLSRPV
                     MKAYKAMLRATGALEAATTDAED"
     gene            complement(6758..8455)
                     /locus_tag="PLH85_05195"
     CDS             complement(6758..8455)
                     /locus_tag="PLH85_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB63987.1"
                     /translation="MKLHLERPHVEALVGAFPRLAWLQEQLRFGHRAEVGLLQLEADE
                     LRFVETLLAGAGPEQHRRAAQIATVRKALGAGGVRFEAEDLEMMVPVLARYLAEDALH
                     GWLFSLTGPDRPTAWVVTRLDFTPAGNEEVGKLLIELKANARGKVITTNLRILAGDIA
                     GRTVGEILIAKGYLKETPELVAAYEEMAERYFDWRARYGEQFSGRGTAIFAENPTATH
                     RDTDWTRKEQVILSSSGGPARLVNDEGILADRELTLAAPGDILGPYLRRAAKSLRYKA
                     DDETEATRDTLPAGLFTRLPVHFFILMFHLDLHHYLWVHVADVTPYAYTPALKQKLIL
                     PEEQTDLIDILTAEMDLLTEDIVAGKSGGTTVLCAGPPGVGKTLTAEVYAEIIRRPLY
                     RVHSGQLGLNVAAMETALKDVLTRAQRWGAVMLIDEADVYIKRRDDNITMNAVVGVFL
                     RVLEYFNGLLFLTTNRIDDIDEAIVSRCIALIKYAPPDAEARSRIWQVMGEQFGLDLD
                     AAHIARLVALFPDATGRDIKGLAKLVAKYCARKACPADLDAFIRCATFRGLEVAHVH"
     gene            complement(8572..9471)
                     /locus_tag="PLH85_05200"
     CDS             complement(8572..9471)
                     /locus_tag="PLH85_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB63988.1"
                     /translation="MHYDLTDLRLFAALAEEASLTRGAARAHLAPSSASHRLARLEES
                     LGVALFERHARGLRPTRAGESLLGHARQVFAQLEQMHADLSPFARGVRGHVSLYANTN
                     AINCFLPDDLGEFLRGSPQIRITLKEMTSPEILRCVAAGEAEIGVVAGEIPPDSLIAH
                     PYRQDRIVLVTPPGHPLSARARVRIAEALDQPFVMLHAGSAFHTGLMNYVAETGGRMD
                     VRIEVRSFEAVLRLAGAGVGLGLVPRSSAERIGRQRPFAIVDVDEPWAQRDLTIVVRR
                     DVALSPHADALVEALRHGGVSAS"
     gene            9571..10467
                     /locus_tag="PLH85_05205"
     CDS             9571..10467
                     /locus_tag="PLH85_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB63989.1"
                     /translation="MSAAGHRLGVLFATLGAVGFSFKAILIKLAYPYGVDAVTLLALR
                     MGFSLPFFVAIGWADARRRQPAALGGRDVLLLLGLGTSGYYLASYLDFLGLQYISAAL
                     ERLILFSYPTLVVVFSAIFLGKRITGRALASIALCYAGIGLAVAHDLKVAGSGRDVWI
                     GGGLVFASAVSYALYLMGNGQAVARLGAARVTAWASTAACLLCLVHFGLTRPLGLLVQ
                     PAQVLGMAAAMAIFSTVLPVWLVSEAMRRLGAGPVSLIGTLGPVVTLFLGWLILAEPI
                     GVFQIAGGTLVVAGVLLVGKRG"
     gene            10572..11666
                     /gene="trmA"
                     /locus_tag="PLH85_05210"
     CDS             10572..11666
                     /gene="trmA"
                     /locus_tag="PLH85_05210"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - S-adenosylmethionine-dependent
                     tRNA (m5U54) methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HNB63990.1"
                     /translation="MPLPRFDPADYDAQLAAKVARLRADFAPFGLPEPAVFRSEPLHY
                     RLRAEFRLWHREGRVDYAMFDPENPRQPVILDDFPAAAEPIHAAMPRLRALLCASEVL
                     RKRIFQVEFLATLSGELMISLVYHRPLDEAWTTAARALAAELGAQIIGRSRGQKIVLE
                     RDWLLEDFEVDGLRRRYQQIEGSFTQPNGGVNRQMLAWARARVAGVGGDLLELYCGNG
                     NFTLALAPCFERVLATELSKSSVHAARYNIALNDVANVTLVRMSSDEISDALAGGRAY
                     TRLRDVDLAGYRFSTLFVDPPRSGLDPATVALARGFGRILYISCNPQTLRDNVAALSA
                     THAIEAAAAFDQFPYTHHLECGVMLRRRAD"
     gene            11693..12034
                     /locus_tag="PLH85_05215"
     CDS             11693..12034
                     /locus_tag="PLH85_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNB63991.1"
                     /translation="MSALPPCPQCGSEFTYEDGAMLVCPECAHEWSASAAPEGGETAR
                     VVKDAHGTVLQDGDSVTVIKDLKVKGSSSVVKVGTKVKNIRLVDGDHDIDCRIDGFGA
                     MQLKSEFVKKA"
     gene            complement(12082..12462)
                     /locus_tag="PLH85_05220"
     CDS             complement(12082..12462)
                     /locus_tag="PLH85_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63992.1"
                     /translation="MPRDLPLPDTITDRGLRVALIFALAGERLNQFLEHGRWLTDAQG
                     ATLAADWLRRGRRTLERAEREHLSALSDRLARQVADSVSREAALYIAHEMAEALDPNY
                     VSDIARSLMAECERLLDADSAAGV"
     gene            12616..12870
                     /locus_tag="PLH85_05225"
     CDS             12616..12870
                     /locus_tag="PLH85_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB63993.1"
                     /translation="MNTTCDCRGLPVAPGDTVRVLAVTLDPDLDDDDADMFRDMIGAT
                     CEVERIDGDGAAWVAVWWNGFDGPLLTTVGLDPAQMEKMA"
     gene            complement(12979..13713)
                     /locus_tag="PLH85_05230"
     CDS             complement(12979..13713)
                     /locus_tag="PLH85_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNB63994.1"
                     /translation="METATAPISREVALRIALAARAMPNATLPRLIELLQRQLGEEIQ
                     LDDLRKVTVTQLKTGFASADGEEDGEDIGIGLEAMKLAVRILWGETQGDDSLPRIQPY
                     TEGDMPGSVRVAIASDQGDTLSGHFGSCLRFLVYQVSPDEIRLVDIRDTMDAEFAPDK
                     NLWRAQLIGDCQVAYVVSIGGPAAAKVIRADIYPIKLPDGGKAEEVLVRFQQAMRESP
                     PPWLLKILGVSAEKRLKNYDASAADE"
     gene            complement(13859..15370)
                     /locus_tag="PLH85_05235"
     CDS             complement(13859..15370)
                     /locus_tag="PLH85_05235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB63995.1"
                     /translation="MTAAQRLRELLLPLVAGFGALALTLVLWQHEQQANQAAMAAHFD
                     ASVRQTAARIDQRIAVYTQMLRGVQGFFRASDLVNREDFATYVDALAQGPEFAGLQSL
                     VYSRAVPGPDGLHAPMIYAAPDLTGNQRAVGHDQMIDAARRAALLHARDSGTLAISQR
                     LRLHSDTQDGPESAFILYLALYRHDRPLNTQAERRDALTGWVQAAFRMKDLMSSLAGE
                     TPPGLDVQLYDGIEPRPDALLYDSRGGQDAAAPVRFEGREFVAAAGHDWCLVVRSQPE
                     FERRFGSKAAEIVLIAGISLSALIAALIWQLITCQTRAHAQARSMTQELRENAEVMRL
                     MAQHDPLTLLPNRALFSDRLQSVLARARRDTTRAALMFVDLDRFKPVNDAYGHAVGDD
                     LLREVSRRMKLCLRESDTLARLGGDEFVVLLAPVDAADDARIVAQRILQTVEEPFYIA
                     DHRLSVSASIGIALYPDQGVDDLALMKAADDAMYVAKDRGRGRMVFAGDPAPA"
     gene            complement(15372..16151)
                     /locus_tag="PLH85_05240"
     CDS             complement(15372..16151)
                     /locus_tag="PLH85_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNB63996.1"
                     /translation="MLLYCAFVCLMLCLTRPAAADGPDCTRPRTLALHEHGLLYSGAS
                     GEGIDKDFADELIRRSGCPVNVTVLPRARIWQLIETGALDFSLSGITNDARERFAAFA
                     WYFSNRYTLLVRKDAGVTRIDELDARPALRLGAIRSFRYGPTANRLVDRLAQTGRVIH
                     ANTLDTLYRALATDHIQAMIIEPFDIPILERARLAEQTRTLEFPDPPIPHGLIMSRAA
                     VSPEEQARWRALVDGMRADGSVERIFGKYFKPDLARAMTRF"
     gene            complement(16337..18151)
                     /locus_tag="PLH85_05245"
     CDS             complement(16337..18151)
                     /locus_tag="PLH85_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNB63997.1"
                     /translation="MRRSPSGPPPAPATGDRHDWQTIRQLLPYLWAYKGRVLFALVCL
                     ISAKFANVSVPLIFKRLVDDLDLSRGQALIVVPAALLLAYGVLRFSTSLFTELREIIF
                     ARVTQEAVREISLQVFHHLHALSLRFHLERQTGGLTRDIERGTRSIGSLISYTLYSIL
                     PTLVEIGLVMGILLTKYEPSFAAITAATLTCYIVFTVRVTHWRTALRRRVNELDSAAN
                     ARAIDSLINFETVKYFNNEAFETRRYDAELARWTEAQIRNQQSLSLLNMGQAAIIAVG
                     VTAMMWRAALGVAGGQMTLGDLVLVNAFLIQLYVPLNFLGVLYREIRQALTDIERMFG
                     LLDARREVADRTDARALARGPAEVRFDHVAFAYEPARAILHQVDFAIPAGHTVAVVGH
                     SGSGKSTLARLLFRFYDVQGGAVRINGEDIRALTQDSLRAAIGIVPQDTVLFNDTLYY
                     NVQYGRPDAPRAEIEAAARAAQLEDFIARLPEGWETRVGERGLKLSGGEKQRVAIART
                     LLKNPDILIFDEATSALDSKTEKAIQAQLDAAARGRTTLVIAHRLSTVMNADEILVLD
                     QGHIVERGDHASLLARNGAYAQMWALQQEERAAEEAAV"
     gene            complement(18197..18829)
                     /locus_tag="PLH85_05250"
     CDS             complement(18197..18829)
                     /locus_tag="PLH85_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNB63998.1"
                     /translation="MPSFDTLLAFLGLSIALTLAPGPDNLMVLGQALAQGWRAGLAIA
                     LGCALGCLTHLTWTVLGVAALLAASPRAFFALRVAGAAWLLGLAVQALRGSRPVRAGD
                     AAAVPWRRSLRRGFVANALNPKVALFFLAFLPQFVDPARGDTARQMIVLGLVFSAQTV
                     VVFGGIALASGALGHALARHPALGPWLDRLCGLIFAGIALRLLAEPSAGA"
     gene            complement(18905..20035)
                     /locus_tag="PLH85_05255"
     CDS             complement(18905..20035)
                     /locus_tag="PLH85_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB63999.1"
                     /translation="MWIARHRFEDMQRRLDGAQRQEGALNAEIDALRAQLAARAAPSP
                     QDNEAAMLRAVITRMAEFGTSLTDAQGSLGEMARLLRDEKEKAVEAAHVSLASGQATT
                     EIAANLQRLADESSQSAREVDTLAQRADEIGTIVKLIHGVADQTNLLALNAAIEAARA
                     GEAGRGFAVVADEVRKLAERTSTATNEISALVNRIRDDSARARSAMESLSRAAGEFSA
                     RGQLATADMQRLMTLSGNMEGVIAGSAMKSFVEVAKIDHIVFKFRIYLAVFGLQPLDV
                     GALPDHTACRLGKWYYTGEGQACFSRLPGYREVEPPHIEVHRAGADVLRALTAGNTVD
                     LVRQLDRMEQASLRVLDHLQRMGEAAVGDPSVLCHAGDGHAH"
     gene            20216..20833
                     /locus_tag="PLH85_05260"
     CDS             20216..20833
                     /locus_tag="PLH85_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNB64000.1"
                     /translation="MNGRDERLPAALLGLVALVLAVSAIAPYDRATWWMEVAPVFLVA
                     PLLIASHRRFPLTPLLQTLIALHALVLIVGGVWTYARVPFGFWLQDVFHLARNPYDRI
                     GHFMQGFVPALAAREVLLRRGIVRGRGWLFFLVTCVCLAVSALYELIEWGAAVALGQG
                     ADAFLGTQGDPWDTQNDMAMALVGALVAQIGLGGWHARQIARLRL"
     gene            20962..22569
                     /locus_tag="PLH85_05265"
     CDS             20962..22569
                     /locus_tag="PLH85_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HNB64001.1"
                     /translation="MPDLPVMPPLEAAPAEPPAAPITPADLVLAYHRRTRHRFDAYAR
                     GPSTLDWDAQPAPFRHFGGTRRIRLPLACAEACAAPPATPAAADLTALGTLLHLSFGI
                     TAWKRLGPDRWAVRANPSSGNLHPVEAYVLVAGLPDLEDGLYHYLPDVHALELRAAHP
                     RPFAAGPALAVGLTSVMWREMWKYGERAFRYCQLDTGHALACLHYAAALLGWRVSDRP
                     GLASAALARVLGTDRDEDFPGRRPDVSREEVELLACVGPADGLPVVTADALAEAADAA
                     RWQGVASEVDRHPMFRWPVVDEVAAASRRVSGVPVTPLPVAIPRPASARHILQRRSAQ
                     RFDPAHVMPREDFVRLLDALRPVAGGRLGLALFIHRVDGLAPGVYLLARADWLRPQPT
                     RAGASALQPVTGCPDLYCLSTSESVPLRRLARSLHCHQDIAANACIAFGLLAQLEREV
                     ADDPARYRDLLREAGFLGQVLYLEAEAMGLRGTGVGCYFDDPVHELVIAGQRDAHSLY
                     HFTIGLPVDDGRIETGPAYPATLEMPS"
     gene            22566..23390
                     /locus_tag="PLH85_05270"
     CDS             22566..23390
                     /locus_tag="PLH85_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNB64002.1"
                     /translation="MNASATAPLSAKFQRISAAEAAALLRRPEGAPRPVLFDVRDRAS
                     YERAHVSGAEHLTDAHLGAALRRVPKSAAVMIYCYHGNASQVYAETFADFRFAEVYSV
                     DGGYEPLAAALALLDGVRLAPRLPDGASADLRAFVLRHAFDPADLDAPRAQGLTPLMR
                     AALQGQAELVGELLAAGVEVDRRNGDGNNALWLACVSRDAATVAALVAAGIDLDNRND
                     MGATCLMYAASSGRPTMVEALLKAGADPYVRNFDDARAVDLCATLECLRLLRHTGN"
     gene            23427..23747
                     /locus_tag="PLH85_05275"
     CDS             23427..23747
                     /locus_tag="PLH85_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64003.1"
                     /translation="MSKYRSIALLMEAVEAPLAAAGVVAGEAWRADEQAVGFFSHADV
                     GLAAYVFTYGQRSGRYGINLDFPLPAEGGPLLTVPTSAEDLPLAAVIALLLEHFDVPV
                     LEAA"
     gene            complement(23955..24341)
                     /gene="apaG"
                     /locus_tag="PLH85_05280"
     CDS             complement(23955..24341)
                     /gene="apaG"
                     /locus_tag="PLH85_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNB64004.1"
                     /translation="MTTLPDPDSIQVKAVARYLADQSDEAADRYVFAYHITIRNTGDA
                     PAQLLSRHWIITDAHGTIQEVEGSGVIGQQPTLKPGEAFEYTSGCVLTTPVGTMKGSY
                     RMQTEDGVDFNATIPEFTLAMPRTLH"
     gene            24478..25170
                     /gene="rpe"
                     /locus_tag="PLH85_05285"
     CDS             24478..25170
                     /gene="rpe"
                     /locus_tag="PLH85_05285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNB64005.1"
                     /translation="MSFRIAPSLLSANFAQLGAEVANVIAAGADWIHFDVMDNHYVPN
                     LTIGPLVCEAIRPCTTAPIDVHLMVKPVDRIVPDFAKAGADIITFHPEASEHIDRTLG
                     LIRDSGCQAGLVFNPATPLHWMDHVMDKLDVVLLMSVNPGFGGQSFIPGTLDKLREAR
                     AKIDAYTARSGRPILLEIDGGVKVDNIAEIARAGADTFVAGSAVFGAGRDTDPHRYDS
                     VIGALRGALAGV"
     gene            25172..25825
                     /locus_tag="PLH85_05290"
     CDS             25172..25825
                     /locus_tag="PLH85_05290"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNB64006.1"
                     /translation="MSPRHAVLFDLDGTLLDSIPDLAEACQRMMRELGHPPHDLETIR
                     SFVGKGMVNLVTRCLGDASAEAVDAAVAVFRRHYAAVNGDATTLYPGVVPALEALAAR
                     GVPLACVTNKPAAFTEPLLARCGIADYFAVTVSGDTLAEKKPHPAPLQHACRLLGVAP
                     AAALMVGDSANDAEAARAAGMPVLLVTYGYSEGRPVDSIDCDGLLSSLEDVLRHLPA"
     gene            26019..27491
                     /gene="trpE"
                     /locus_tag="PLH85_05295"
     CDS             26019..27491
                     /gene="trpE"
                     /locus_tag="PLH85_05295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNB64007.1"
                     /translation="MTEAEFNALAAQGYNRIPVTVDTFADLDTPLSVYLKLANAPNTY
                     LLESVQGGERFGRYSIIGLACSTRIEARGRSVMLLTNDRVVERRDDTNPLDYVAEFKA
                     RIKVPPRDRLPRFAGGLVGCFGYDTVRYIEPRLASTHKPDALGTPDVLLLLSEEIAIV
                     DNLSGKLTLVVYAEPQVPDAYARATRRLKELIGRLREPVSIPAEAPGAPAEAVSSFGE
                     AAFKDAVRKAKHYIVEGDIMQVVLAQRMSKPFAASPLALYRAIRSLNPSPYMFYFNFE
                     DFQVVGASPEILVRLEDNVVTVRPIAGTRKRGATHDEDLALEAELLADEKERAEHLQL
                     LDLGRNDCGRVAGTGSVRVTEQFTVERYSHVMHIVSNVEGRLRDGLDALAVLRATFPA
                     GTVSGAPKLRAMEIIDELEPTKRGIYAGSVGYVGFHGDMDLAIAIRTAVLKDGHIHVQ
                     AGAGIVADSNPDAEWEETQNKARAMLRAAEIAEAGLDTPL"
     gene            complement(27596..28150)
                     /locus_tag="PLH85_05300"
     CDS             complement(27596..28150)
                     /locus_tag="PLH85_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64008.1"
                     /translation="MKNARILLTAVTLAAATAPAFAGDWKFLPILDKDYRPNFVASVT
                     GGMLDPKHGSSDTALGLELAMNCGLFQTPTGVVRTKLSWNRYDKDGVTLNTVELNPRW
                     TIPVAQNLTFGVGPGIGYVKADAGGRTTDLFAWQAGADLDYRIGQVNLGLGARWQDTV
                     NKDIAPGMQGAENWLVQAKVGIAF"
     gene            28336..28905
                     /locus_tag="PLH85_05305"
     CDS             28336..28905
                     /locus_tag="PLH85_05305"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNB64009.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGADVRVFRNDEITVEQIAELAP
                     AQIVVSPGPCSPAEAGISVAAIQTFAGKIPLLGVCLGHQSIGAAFGGRIVHAKQLMHG
                     KVSPVHHLDQGVFKGLPNPLTCTRYHSLAIERETLPDCLEVTAWTDDGEIMGVRHKTL
                     AVEGVQFHPESILTERGHDMLRNFLDASR"
     gene            28918..29949
                     /gene="trpD"
                     /locus_tag="PLH85_05310"
     CDS             28918..29949
                     /gene="trpD"
                     /locus_tag="PLH85_05310"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNB64010.1"
                     /translation="MNFTPQEALQRTIEHREIFYDEMLSLMRQIMAGEVSPVMTAAIL
                     TGLRVKKETIGEIAAAATVMRELSSKVIVPPPHEHFLDVVGTGGDGAHTFNISTATIF
                     VAAAAGARVAKHGGRSVSSKSGSADVLEALGVNLNLNAEQVAECIARTGIGFMFAPNH
                     HSAMKNVAPVRREMGVRTLFNILGPLTNPAGAPNTLLGVFHADLVGICVRVMQRLGAH
                     HVLVVHGRDGMDEVSLGAATLVGELKDGEIREYEIHPEDFGLAMMSSRRLQVSTADES
                     RAMLLSVLDNVPSAAREIVILNAGVALYTANLADSIGAGIALAREAIASGAARARLDA
                     FVAATRSFA"
     gene            29957..30745
                     /gene="trpC"
                     /locus_tag="PLH85_05315"
     CDS             29957..30745
                     /gene="trpC"
                     /locus_tag="PLH85_05315"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNB64011.1"
                     /translation="MSDILNKILAVKRDEVAAARARVSDAAVRQLAADQPAPRDFVGS
                     MRARLGAGRSAVIAEIKKASPSKGVIRADFRPAEIAPSYERAGAACLSVLTDRQFFQG
                     APDYLQAARAACTLPVLRKDFLVDPYQVFEARAMGADAILLIVSALSLAEMQAMEAIA
                     ESLGMAVLVEVHDGAELDLALQLRTPLVGINNRNLRTFEVSLQTTLDLLPRIPAERIV
                     VTESGILQPADVALMREHRVNAFLVGEAFMRADDPGAGLAALFA"
     gene            30752..34783
                     /locus_tag="PLH85_05320"
     CDS             30752..34783
                     /locus_tag="PLH85_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNB64012.1"
                     /translation="MGDAAPPPDPATPPPAQAPSPARRGAADAFARLLRATLGGAFAT
                     LALLIATLGGLGGTEAGLRLLAEGATALSGGDLRIEGAEGHLLGHVRLAALRLHRADL
                     HLDLTRVDLDWTPAALLHGRLDIAALSAADVVVATPPDDTPATLPASLELPVAVHVGR
                     LHLDRLRIGALRDGAAAAPDVDLTEVEAAADSDGRLHRVATARARTAFGALAAQGQLD
                     GRRPFALRAHADLATRQGGDAYTVQADAHGDLQTLTIDARAAGAGLSGEAHVAATPFA
                     AVPFRSARLAIRDLDPARFHAAAPGARLDVSADLEPVASATDGAWSVAGPVRLLNRAP
                     GRYDAGRLPVASLAALLRWGEGRLSLTDLDLRLPRGEVRGGASWVRAAAGAPDGDIGR
                     VEADLQVRGVALPALDGRLPAATLAGRVQARADAGGQTVEADLRDPRVALRLSARHAD
                     GAVTVQELLARAGKARLSVRGAADLAGDGRFELKGRLDHFDPHAFFTEAPAADLTLDL
                     EARGRRSPQMAATLSFQLAPSTLEGRPLVGHGTLAVDGRRLSDADLTLAVAANRLSAR
                     GRYGAPGDALDLHLEAPDLAALGHTLSGRVTAAAVLRGTPARPAGELELSGEGLAYGT
                     VHVEGVNGRGRLEEGADAPLNLLLNLAGVRTGADLEHGLRRASLVVDGRRSAHRAHLE
                     AHTPGADALELELSGGLGDALRWQGRLDRLRVLGPHATTLAAPVPLVLAPAEVRIGAA
                     ELRSEAGAALRLDETRWADGEIVARGRLSGLPVGIGLDAAGANRAALGDTLRMGGDWD
                     IHVGGHVDGTVHLFRERGDLLVLGDAPVRLGLDEVQIVVNAVASRLAFGFSVSGAQLG
                     KASGAGTALAERTADGGVRLVPDAPLLGSAQLDMPSIAWTGPLADPNLKTAGRLKADF
                     TLSGTPAQPVTEGRIRGEALAFAMADQGLNLSGGTLVADFNRDRLRVERFDFASPNRV
                     KPNEGRIDVARLTRTPGSLQVKGEVALASGAGAFSFQAERLPLLQRPDQWLVLSGAGE
                     VHSGWNSARIRGRLTADAGYVELSRTPAPSLGDDVEVVRPEQARPPAVKPFSTAVDLA
                     IGLGDALYVRALGLDTRLAGELRLRAGDGQPLAMTGVINTQGGVYEGYGQKLDIERGI
                     VTFTGAVSNPSLNVVALRKGLSVEAGVAITGTARRPLVRLVSEPNVPDPEKLAWIVLG
                     RPPDQGSGGELALLLPAAQALLGTDSMSSGLVRALGFDEFSFGSSVDSRQRIQTSSVA
                     TGSVTGTTSSAGTTTGSESTAGQVVSIGKRLSARALLSFEQSVTGASSIVKLSYQLTR
                     RIAVVGRAGSENALDAVFTLSFR"
     gene            34889..35614
                     /locus_tag="PLH85_05325"
     CDS             34889..35614
                     /locus_tag="PLH85_05325"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNB64013.1"
                     /translation="MTEPLQPCRIPVSAVWAALAEGGRDFRRHPGPAALFASVFAALG
                     LGLFGVLAAYDLHALRLPLAGGFLLVGPALLAGFFAVRRVAATRPPRLADLLGGFAAA
                     PRGVWALAAVCALLFLIWMSDAATVYSFMIGDTRPGADQVWRFHAWTSLMGGVFALIV
                     FHITAFAVPLLLDRRATLVGAVVASVRGIWTNGPAMATWAGVLAVAIIATVFVPPLLG
                     VSLPWLAFVSDLLYLRVYPSRPK"
     gene            35639..36181
                     /gene="msrA"
                     /locus_tag="PLH85_05330"
     CDS             35639..36181
                     /gene="msrA"
                     /locus_tag="PLH85_05330"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNB64014.1"
                     /translation="MTDTTSETAILAGGCFWCLEAVFLLAEGVHTVTSGYIGGHVDAP
                     TYHQVCDGTTGHAEAIRIEFDPARITFDDLLDIFFAIHDPTTLNRQGNDVGTQYRSAI
                     FATTPAQAAAAQARIAALAGAYHAPIVTEVVPAPVFWPAEPYHHDYFSRNGHQPYCQV
                     IVAPKVQKFLRAFPQRVRST"
     gene            36218..36562
                     /locus_tag="PLH85_05335"
     CDS             36218..36562
                     /locus_tag="PLH85_05335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB64015.1"
                     /translation="MSEVTTASGLVIEDLEVGTGPEASAGQRVQVHYTGWLTDGRKFD
                     SSKDRNDPFVFPLGARHVIAGWDEGVQGMKVGGRRKLTIPPNLGYGARGAGGVIPPNA
                     TLVFEVELLAVA"
     gene            complement(36675..38909)
                     /locus_tag="PLH85_05340"
     CDS             complement(36675..38909)
                     /locus_tag="PLH85_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB64016.1"
                     /translation="MTALPFQPRRLALLLLSSCPALLATVPARAAEGPAALPEVEVKA
                     TRLRPLAEGAGPTSARLAGLKATTSDTAALLQDVPGVALAGAGGLSSLPVVRGLPDDR
                     NRVQVDGMDLISACGNHMNPPLSYLESFRVGAIQVFTGAVPVSLGGDSIGATVVAESA
                     PPPFAAPGQGTVAGGALGAFYRSNGAARGVQGEAHWASERLSVRYAGGVSTSDNLRAA
                     KDFKPATQATWTRSGTHPLDANEIGSTAYTLRSHSLGFALRSGEHMADLKVSVQEIPR
                     QGFPNQHMDMTDNHSTQVNLGYTGSFAWGSLQARLYNEETRHRMDFGDDKLYWYGSAR
                     NVAGMPMETHGVNTGARVRADVNLNERDILRVGSEYQRYHLNDWWPPVANAMMMSPNT
                     FQNLHNGQRDRMDVFAEWEAAWSPRWQTLAGLRSSTVRMDADPVQGYNTMAMMYGADA
                     ARFNAASRARTDHNLDATLLARFTPDAGRSFDAGYARKTRSPSLYERYTWSTNGMAMT
                     MNNWVNDGNGYVGSIDLAPEVAHTLSASADLHDAGQARWGVKLQSFYTYVNNYINARC
                     LTSCPPNQFNYLRLVNTDARLYGADLSGYRVLDGGETLGPITGRGTLAYVHGKDTHSD
                     DGLYNQMPLNARLSLEQKHGPWTTAVEEVLVGAKRAVSQVRNEIATGGYALLNLRSSY
                     DTRHYRVDLGLENALNRFYASPLGGAYIGQGTTMSLNGAGAPYGIAVPGMGRSVYVAL
                     TVRY"
     gene            complement(39486..>39716)
                     /locus_tag="PLH85_05345"
     CDS             complement(39486..>39716)
                     /locus_tag="PLH85_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64017.1"
                     /translation="RHASVGVAPATRPWLHVTQGFPTGMHDDRGGTIAYFLGTRGFLN
                     PTIASFLGSTAYFFGNSGFSRPMIAYFLGTIA"
BASE COUNT         5587 a        13930 c        14020 g         6179 t
ORIGIN      
        1 gcgccgccct tgtcgccgaa ggacatgacg gcggtggcgc tgccgtggat gcccagcttg
       61 tgttcgatgg acgagcagat caggtcgttg cgctcaccca ggctgccgtc ggcattgacc
      121 aggaacttgg gggcgatgaa cagggagatg cccttcacgc ccggcggggc gtccggcagg
      181 cgggccagca cgaggtggat gatgttctcc gccatgtcgt gctcacccca ggtgatgaag
      241 atcttggtgc cggtgatggc gtagctgccg tcgccgcggg ggtcggcctt gctgcggatg
      301 gcggccaggt cggagccggc ctggggttcg gtgaggttca tggtgccggt ccatttgccg
      361 gagaccatgt tgggcagata ggtggccttg agttcgtcgg aggcgtggtg ggcgatggct
      421 tccacggcgc cgagggtgag catctggcac agggagaagg agatgctggc ggacttccac
      481 atctccagca cggcggtgga gacggtcacc ggcaggccct ggccgccgaa ctcggtggag
      541 gcgggcatgc cgttccagcc ggtctcgcag aaggtggcgt aggcttcctt gaagccgtcg
      601 gcggtggtga ccacgccgtt gtcccacttg ttgccctgct tgtcgccgac ccggttgagg
      661 ggatcgacca cttcggaggc aaacttggcg gcctcgtcga ggatggcctc gaccaggtcc
      721 tggctcactt cctcgttgcc gggcagggtg gcgatctcgt ccagaccggc cagttccttc
      781 atgtcaaaca gcatgtcctt cacgggggcg acgtaggtgc tcatgggggc tccttgaaat
      841 ttcgggttcc ggccgcgggg gccggggcgc gacgcaacgg tcgcgcccgg ggtttcggat
      901 cgattcggga ttacagggcg gcgaccagct ggggcaccgc ttcgaacaga tcggccacga
      961 ggccgtagtc ggccacctgg aagatcgggg cttccggatc cttgttgatc gccacgatca
     1021 ccttggaatc cttcatgccg gccaggtgct ggatggcgcc ggagatgccc accgccacat
     1081 agagctgggg cgcgacgatc ttgccggtct ggcccacctg gtagtcgttg ggcacgaagc
     1141 cggcgtccac cgccgcgcgg gacgcaccca gggcggcgcc gagcttgtcg gccaggggtt
     1201 cgagcacgct gcggtaggcg tcgccgctgc ccagaccgcg gccgccggag acgatgatct
     1261 tggcggcgcc cagttcggga cgggcggaga cggtcagttc gcggcccacc aggctgctga
     1321 ggcccaggtc ggggccggcg gcgacggctt ccagggtcgc gctgccgccc ggttcgacgg
     1381 cgtcgaaggc ggtggtgcgc acggtgacga ccttgatggc gtcggcgctc ttgacggtgg
     1441 ccagggcgtt gccggcgtag atcgggcgca cgaaggtgtc ggcggattcg atggcgacga
     1501 tgtcggagat ctgggcgaca tcgagcagcg cggcgacgcg gggcaggacg ttcttgccca
     1561 gggtggtggc gggggccagc acatggctgt agccgccggt cttgatgaca tccaccagca
     1621 gcgcggcgag gttctcggcg ccctggtcgg cgtaggcggg cgcgtcggcg accttgacgg
     1681 tgctgacgcc ggccagcttg gtggcggcgt cggcggccgc ggcgcaggcg gcgccggcca
     1741 ccagcacatc tacaggcgcg cccagcttgg cggcggcggt gacggtgttg agggtggcgg
     1801 ccttgaggct gccgtggtcg tgttcggcga tgacgagtgc gcgcatgttc agatcacctt
     1861 cgcttcgttc ttgagtttgt tcaccagctg ggccacgtcg cccaccatga cgccggcgct
     1921 gcgggcggcc ggctccacca ccttgagggt ggcgaggcgc ggggccacgt ccacgcccag
     1981 gtcggccggc ttgacggttt ccagcggctt cttcttggcc ttcatgatgt tgggcagggt
     2041 cgcgtagcgc ggctcgttga ggcgcaggtc ggtggtgatc acggccggca gcgggaggga
     2101 gagggtctcc aggccgccgt cgatctcacg ggtgacggtg acccggtcgg cgcccagctc
     2161 caccttggag gcaaaggtgg cctgcggcca gccgagcagg gcggcgagca tctggccggt
     2221 ctggttggcg tcgtcgtcga tggcctgctt gccgaggatc accagctggg gctgctcctt
     2281 ggcgaccacg gccttgagca gcttggcgac ggcgagcggt tgcaggtcgg cgtcggtctc
     2341 ggccaggatg ccccggtcgg cgccgatggc catggcggtg cgcagggttt cctggcactg
     2401 ggcgacgccg gcggagaagg cgacgacctc ggtggccttc tgggcttcct tgaggcgcac
     2461 ggcctcctcg acggcgatct cgtcgaaggg gttcatggcc atcttcacgt tggccagctc
     2521 cacgccgctc tggtcggcct tgacgcggat cttgacgttg taatcgacga cccgtttgac
     2581 gggcacgaga atcttcatgg ctgctttttc tccggtggtg agctttcagt ccaatgcggc
     2641 gggcgatacg acgacggcgc gcgggtgcgc cgggggctgc ccatgggcca tgaagacggt
     2701 ggcggagccg ggtcggacgg tgcgggaggc gggggcggac ggcggctctg gcgcgaatgg
     2761 ccggaacggt ccggggtgat gcgggacggg ggacatccga ttctcctcct cgtggtgcac
     2821 ggtcgccgac gatcggcgct gcggcggctc tgtggccctt cttttttccc cggctcggga
     2881 cggacgcccg gggatgggga cggagcttgg caggagaacg tgtcgcaact ttcgccaaaa
     2941 tgtactgaat cttgtcggcc gttccggcgt gcggacggcg ggctagaatc cgcccgaagg
     3001 caggaggcga aggggttgat gggggctatg aacgcactgg acgacagcgc ggccgcgggc
     3061 gacgcggcgc agagcggcgc agaaggggac cggcgttacc gggagatgct cggggccggt
     3121 ctggacctgc tcgaccaggg cgtgacggtc ttcgacgccg atctgcgcat ggtggcgtgg
     3181 aacgccgcct tcctgcgcct gctggagttc cccgccgagc tggcgcggcc cggcgtgtcc
     3241 ttcgacgact tcatccgcta caacgcccgc cgcggcgaat acgggcccgg cgatccggag
     3301 gcccaggtgg cggagcgggt cacccgtgcc cgcagcttcc aggcccacac caccgagcgg
     3361 gtgcgcccga acggccaggt cctgctgatc cgcggtgcgc cgctgcccca ccggggcttc
     3421 atcacgctgt ataccgacat caccgaggag cgccgcgccc aggccgagct ggaggaacac
     3481 atccgcgtgc gcaccgaggc cctgacccgc gccaacgcgg agctcaccgc ggcggtggag
     3541 tccaacaagg ccatcgccgc cgccttgcag cgcagcgagc agcgcctgcg tgagatcacc
     3601 gacgcgatcc ccgccgcggt ggcctatttc gaccagaact ggacctaccg ctatgccaac
     3661 aagggctatg ccgagtggtt cggctggtcg gcggagcgca tcgaggggcg cagcatccgc
     3721 gaggtgatcg gccaggaagt gttcgacatc gtctcgccct acgtgacccg cgccctggcc
     3781 ggcgagcgca tggcctacga atacaccctg accggccgcg acggcggacc ggcccatgcc
     3841 cgcagcaccc tggtgccgga tttcggcgcc gacgggcagg ttatcggctg ctatgtgcat
     3901 tcggtggaca tcaccgagcc gcggcgcacc cagatggcgc tgtcccaggc ccagaagatg
     3961 gaagccatcg gccagctcac cggcgggctg gcccacgatt tcaacaacat gctcacggta
     4021 gtgatgggca acctggccgc cctgcgggag aaccatcccc aggaccccct caccgaaaac
     4081 ttcgtcgagc ccgccatgca ggccgcccac cggggcgccg agctgatccg gaggctcctc
     4141 agcttcgccc gcaagcagcc cctggaggcg cggccggtgg aggtcaatga gctgatcctg
     4201 ggcatgtcca agctgatccg ccgctccctg ccggggacca tcgcggtgag cacggagagc
     4261 cgtgagccct gtctggtggc gctggtggac gagcaccagc tcgagaacgc cctcctcaac
     4321 ctggccctca acgcccgtga cgccatgccc aacggcggcg agctgcgcat cgagtcgtcc
     4381 ctggaaaccc tcagcccctc ggccgccgcc gacctggaag tgccgcccgg cccctacgtg
     4441 cagatcgccg tcaccgataa cggcatgggc atggacggca gcaccctggc gcgggtcttc
     4501 gagcccttct tcaccaccaa gcagttcggc atgggcagcg gcctcggcat gtccatggtg
     4561 tacggcttcg tcaagcagtc cggcggcggc gtgcgcatcc gcagccgtca ggcccgcggc
     4621 accacggtgg cgctgctgct gccctgggtc gaggacacgg ccgacagcga ggtggtgccg
     4681 gtcggcgcgc ccgtgtcgga ggccgatctg gcgggcaagc tggtgctgct ggccgaggac
     4741 gacgccgacg tgcgcactgt ggtgcgcatg cagctcgccg agctgggctg cgccgtggtc
     4801 gaggcggaga acggcgccga ggcggcggac atggtggaga acatccccgc catcgccctg
     4861 gtgatctcgg acgtggtgat gcccggcgac atggacggcc gcgccctggc gcgcttcgtg
     4921 cgccgcttcc ggcccgaact gccggtggtg ctgatgagcg gcttcgccga ttcggcggcc
     4981 gccttcgatg cggacctggc cctgcccctg ctggccaagc ccttcacccg cgacaagctg
     5041 ctggccgccc ttggcccgct ggcggcctga ggggagggcc gtcatgaccc gcaacccgga
     5101 gctgatctat gtggtcgagg acgaactgga tgtggcgcgc ctgatccgcg ccgccctgga
     5161 gaagttcggc ttccgcaccg aggtcttccg caccgccgag gccttcttcc acggggtacg
     5221 ccaccgggtg ccggacctgg tgatcctcga cctgggcctg cccgacatgg atggcatggc
     5281 ggtgctccag cagttgcgca gcaagcacac ctgcggcctg ctggtgctca ccgggcgggg
     5341 cgacgtctac gaccgggtga tgggcctcga gctgggcgcc gacgattatg tggtgaaacc
     5401 ctacgagccc cgcgagatga tcgcccgggt gcgcagcatc ctgcgccgct acccgcgtca
     5461 gccggtcgtg ccggccgatc ccacccagcg ggtcgcggag tttgccggct ggcgcttcga
     5521 gaccggcagc aacaccctgg tcgggcccga cggccagcag gagaccctca gcaccgcgga
     5581 ggcccagctc ctgagcgtgc tcctggccca tcccaaccac atcctcaccc gcgagcagct
     5641 cctgggcggg cgcgacgtgt ccgcgctgga ccgcagcatc gacctgcgca tttcgcgcct
     5701 gcgccggcgc ttcgaggaaa atccccagca cgccaagctc atcaagacgg tgtacggcgc
     5761 cggttacctg ctggccgcca ccgtcacctg gtcctgaggc cgcctgcggc aacgcgtcgc
     5821 agggcatcga tcgcccgttg cggcgggcga gccggtaagc ttgcgccttt gccaaactgg
     5881 agcgacagga tgagacatgc ccggatgctg gccctgctgg tcgggacgat gatgatggcc
     5941 ggcgcggcga tggccgccga tgtggaggtg cgtgacgcct gggtgcgggg cgtggtcccg
     6001 gcccagaaga gcacgggcgc cttcatgcag ctcaccagcc cggccggagc gaccctggtg
     6061 ggtgccgcca gcccggccgc ggccaaggtg gaactccacg agatggccat ggaaggcaat
     6121 gtgatgcgca tgcgcccggt gccccgcctg gcgctggcgc ccggccagac cgtggcgctg
     6181 aagcccggcg gctaccacgt gatgctgatc ggcctgaaga agcccctcgt cgacggtgac
     6241 aaggtgccgc tcacgctgct gctggaaggg cgcgacaagc aggtcgacaa ggtcgaggtg
     6301 agcgccgagg tgcggcccct cacggcggcg gcgatggacg agcaccacca ccaccactga
     6361 gcggcaggcc tgccgggtgg cttggcgggt tcagtcctcg gcgtctgtcg tggccgcctc
     6421 cagcgccccg gtggcccgca gcatggcctt gtaggccttc atgacggggc gcgacaggcg
     6481 cgtctcggcg agcaggcgct gctcgtagac ctccatccgc agggcctcgt ccaggccctc
     6541 gcgggcgcgg gcgatgaggg catggaagtc ggcgccgaag cgcgcccccg gccggtcctt
     6601 gagcaggtcg tagagggcgg ccatctgcac cttgtccgtc gggctcatct tgcacttgcc
     6661 gtcgaggatc ttgagcatca ccgtggtggt gcagtccacg tcggacgggg tgaggggcgt
     6721 gactgccgcc caggcggcca ggcgcgcggc gagggcctca gtggacatgg gccacctcca
     6781 ggccgcggaa ggtggcgcag cggatgaagg cgtccaggtc cgcggggcag gccttgcggg
     6841 cgcagtactt ggccaccagc ttggcgagcc ccttgatgtc gcggccggtg gcgtccggga
     6901 acagggcgac caggcgcgcg atatgggccg cgtccagatc cagcccgaac tgttcgccca
     6961 tcacctgcca gatgcggctg cgcgcctcgg cgtccggcgg ggcgtacttg atcagggcga
     7021 tgcagcggct gacgatggcc tcgtcgatgt cgtcgatgcg gttggtggtg aggaagagca
     7081 ggccgttgaa gtattccagc acccgcagga acacgcccac cacggcgttc atggtgatgt
     7141 tgtcgtcgcg ccgcttgatg tagacgtcgg cctcgtcgat gagcatcacc gcgccccagc
     7201 gctgggcgcg ggtgagcacg tccttgaggg ccgtctccat ggccgccacg ttgaggccga
     7261 gctggccgga gtgcacccgg tagagggggc ggcggatgat ctcggcgtac acctcggcgg
     7321 tgagggtctt gcccacgccg ggggggccgg cgcacagcac cgtggtgccg ccggacttgc
     7381 cggcgacgat gtcctcggtg aggaggtcca tctccgcggt gaggatgtcg atgaggtcgg
     7441 tctgctcctc cggcaggatc agcttctgct tgagggccgg cgtgtaggcg tagggcgtca
     7501 cgtcggccac gtgcacccac aggtagtggt gcaggtccag gtggaacatg aggatgaaga
     7561 agtgcaccgg caggcgtgtg aacaggccgg cgggcagggt gtcgcgggtg gcctcggtct
     7621 cgtcgtcggc cttgtagcgc aggctcttgg cggcgcggcg caggtagggg ccgaggatgt
     7681 cgcccggcgc ggcgagggtc agctcacggt cggcgaggat gccttcgtcg ttcaccaggc
     7741 gggccgggcc gccgctggag gacaggatca cctgctcctt gcgcgtccag tccgtgtcgc
     7801 gatgggtggc ggtggggttc tcggcgaaga tcgccgtgcc ccgcccggag aactgctcgc
     7861 cgtagcgggc gcgccagtcg aagtagcgct cggccatctc ttcgtaggcg gccaccagct
     7921 ccggggtctc cttgaggtag cccttggcga tgaggatctc gcccacggtg cgcccggcaa
     7981 tgtcaccggc gaggatgcgc aggttggtgg tgatcacctt gccgcgggcg ttggccttga
     8041 gctcgatgag gagcttgccc acttcctcgt tgccggcggg ggtgaagtcc aggcgcgtga
     8101 ccacccacgc ggtggggcgg tccggcccgg tgagggagaa cagccagccg tgcagggcat
     8161 cctccgccag gtaacgggcg aggaccggca ccatcatctc caggtcttcc gcctcgaagc
     8221 gcacgccgcc ggcgcccaga gccttgcgca cggtggcgat ctgggcggcg cggcggtgct
     8281 gttccggccc ggcgccggcc agcagggtct ccacgaaacg cagttcgtcg gcttccagct
     8341 gcagcaggcc gacctcggcg cggtggccga aacgcagctg ctcctgcaac caggcgaggc
     8401 gggggaaggc ccccaccagg gcttccacgt gggggcgttc gaggtgtagc ttcatcggca
     8461 aggtctccgc ggcggtcgga gcccttcacg caaattccgc gccgcctgcc ggacgcacgc
     8521 aagtgacggg ggcaaaaggg attttgcgcc ggagaggggt gcgacggcgt gtcaggaagc
     8581 cgacacgcca ccgtggcgca gggcctcgac gagggcgtcg gcgtggggcg acagggccac
     8641 gtcgcggcgc accacgatgg tcaggtcgcg ctgcgcccag ggctcgtcca catccacgat
     8701 ggcaaagggg cgctggcggc cgatgcgctc ggccgaggag cgcggcacca ggcccaggcc
     8761 gacacccgcg ccggccagac gcagcaccgc ctcgaagctg cgcacctcga tgcgcacgtc
     8821 catgcgcccg ccggtctcgg ccacgtagtt catcagcccg gtgtggaagg cgctgcccgc
     8881 gtggagcatc acgaagggct ggtcgagggc ttcggcgatg cgcacccgcg cccgtgccga
     8941 cagggggtgg ccgggcggcg tgacgagcac gatgcggtcc tgccggtagg ggtgggcgat
     9001 cagcgaatca ggcgggatct cgcccgccac cacgccgatc tcggcctcgc cagcggccac
     9061 gcagcgcagg atctccgggc tggtcatctc cttgagggtg atgcggatct gaggcgagcc
     9121 gcgcaggaac tcccccaggt cgtcgggcag gaagcagttg atcgcattgg tgttggcgta
     9181 gaggctcacg tggccgcgca cgccgcgggc aaagggactc aggtcggcgt gcatctgttc
     9241 gagctgggcg aacacttgcc tggcgtggcc gagcagggac tcgccggcgc gggtggggcg
     9301 caggccgcgg gcgtggcgtt cgaacagggc cacgccgagg ctctcctcca ggcgggccag
     9361 gcggtgactg gctgaggacg gtgccaggtg ggcgcgggct gcgccgcggg tcaggctggc
     9421 ttcctcggcg agggccgcga agaggcgcag gtcagtcagg tcgtaatgca caaggtttcg
     9481 tctccatcga atgcttggct cggatcttag caattcccac cgtagccggg cgatggccta
     9541 atggtggcct gttccctgct ccgtcttccc atgtccgcag ccggtcatcg cctcggcgtc
     9601 ctctttgcca ccctcggtgc cgtgggcttt tcgttcaagg ccatcctcat caagctcgcc
     9661 tacccctacg gcgtggatgc ggtcaccctg cttgccctgc gcatgggctt ctcgctgccc
     9721 tttttcgtgg cgatcggctg ggccgacgcg cgccgccgtc agcccgcggc cctgggcggg
     9781 cgtgatgtgc tgctcctgct ggggctgggc acctccggct attacctggc gagctacctg
     9841 gacttcctcg gcctgcaata catctctgcg gcgctggagc ggctgatcct gttctcctat
     9901 ccgaccctgg tggtggtgtt ctcggcgatc ttcctgggca agcgcatcac gggccgggcg
     9961 ctggcgtcca tcgccctgtg ttacgcgggc atcggcctcg cggtggccca cgatctgaag
    10021 gtggcgggct cggggcgcga tgtatggatc ggcggcgggc tggtgttcgc cagcgccgtg
    10081 tcctacgccc tgtacctgat gggcaatggg caggcggtgg cgcgcctggg ggcggcgcgg
    10141 gtcactgcgt gggccagcac cgcggcctgc ctgctgtgcc tggtgcattt cgggctgacc
    10201 cggcccctcg gcctgctcgt ccagccggcg caagtgctgg gcatggcggc ggcgatggcg
    10261 atcttctcca cggtgctgcc ggtgtggctg gtgtcggagg ccatgcgtcg cctgggggcc
    10321 gggccggtgt ccctgatcgg cacccttggg ccggtggtga ccctgttcct cggctggctg
    10381 atcctggcgg agccgatcgg ggtcttccag atcgcggggg gcacgctggt ggtggccggc
    10441 gtgctcctgg tgggcaagcg cggctgacgc gcgcggggtg cgcggggggc gtgtccggcg
    10501 tatagtccgc gcttcgccac gcgtgcgggc ctctttccgg gcccgtaccg gcccgttttc
    10561 gctgcatcgc catgcctctg ccccgtttcg accccgccga ctacgacgcc cagcttgccg
    10621 ccaaggtggc gcgcttgcgg gccgatttcg cgcccttcgg cctgcccgag ccggcggtgt
    10681 tccgctctga gcccctgcat taccgcctgc gcgccgagtt ccgcctgtgg caccgggagg
    10741 ggcgcgtgga ttacgccatg ttcgacccgg agaacccgcg ccagccggtg atcctggacg
    10801 attttccggc cgccgcggaa ccgatccacg ccgccatgcc gcgtctgcgg gcgctgctgt
    10861 gcgccagcga ggtgctgcgc aagcggatct tccaggtgga gttcctggcc accctgagcg
    10921 gcgagctgat gatcagcctg gtctatcacc gccccctgga cgaggcctgg accactgccg
    10981 cgcgggccct ggcggccgag ctgggcgccc agatcatcgg ccgcagccgc ggccagaaga
    11041 tcgtactgga acgggactgg ctgctggagg acttcgaggt ggacggcctg cgtcgccgct
    11101 accagcagat cgagggcagc ttcacccagc ccaacggcgg cgtgaaccgc cagatgctgg
    11161 cctgggcgcg ggcccgcgtg gccggcgtgg ggggcgacct gctggagttg tactgtggca
    11221 acggtaactt cacgctggcg ctggcgccct gcttcgagcg ggtgctggcc accgagctga
    11281 gcaagtcctc ggtccatgcg gcgcgctaca acatcgccct caacgatgtc gccaatgtga
    11341 ccctggtgcg tatgtccagc gacgagatca gcgacgccct ggccggcgga cgggcctaca
    11401 cgcggctgcg tgacgtggac ctggcgggct accgcttctc gaccctgttc gtcgatccgc
    11461 cccgcagcgg gctggatccg gccaccgtcg cgctggcgcg gggcttcggg cgcatcctgt
    11521 acatctcgtg caacccccag accctgcgcg acaacgtggc ggccctgtcc gccacccatg
    11581 ccatcgaggc cgccgcggcc ttcgaccagt ttccctacac ccatcacctg gagtgcgggg
    11641 tgatgctgcg tcggcgcgcc gactgaccgc cccttttccg gagaatcgcc ccatgagcgc
    11701 cttgcccccg tgtccccagt gcgggtccga attcacctat gaggacgggg cgatgctcgt
    11761 ctgtcccgaa tgcgcccacg aatggtccgc cagtgcggcc cccgagggcg gcgagactgc
    11821 gcgggtggtg aaggatgccc acggcaccgt gctgcaggac ggggactccg tcaccgtcat
    11881 caaggacctg aaggtgaagg gctcgtcttc ggtggtgaag gtgggcacca aggtgaagaa
    11941 catccgcctg gtggacggcg atcacgacat cgactgccgc atcgacggct tcggcgcgat
    12001 gcagctcaag tcggagttcg tgaagaaggc ctgagttggc aactgagtcg gcaactgagg
    12061 cggccgcgga gtcggccggt ctcagacgcc cgcggcggag tccgcatcca gcaggcgctc
    12121 gcattccgcc atcaggctgc gggcgatgtc cgagacgtag ttcgggtcga gggcttccgc
    12181 catctcgtgg gcgatgtaca gcgccgcctc ccgcgacaca ctgtcggcca cctggcgggc
    12241 gaggcggtcg ctgagagctg acaggtgttc ccgttcggcc cgctccagcg tgcggcgccc
    12301 gcggcgcagc cagtcggcgg ccagggtggc gccctgggcg tcggtgagcc agcggccgtg
    12361 ttccaggaac tggttcaggc gctccccggc gagggcgaag atcagcgcga cgcgcaggcc
    12421 gcggtcggtg atggtgtccg gcaaaggcag gtcgcggggc atgggcggcg tccacaggca
    12481 tcgggaggcg gacgctagca cgattggggc aggctggcac ggggtttgca ccggaagatt
    12541 ccaggcacga tccggtgtcg cattcgatac gccgggcgat gccgccgatt tcgaccgacc
    12601 cctggagatc ccgtcatgaa caccacctgc gactgccgcg gcctgcccgt ggcccccggc
    12661 gacaccgtcc gcgtgctggc cgtcaccctc gacccggacc tggacgacga cgacgcggac
    12721 atgttccgcg acatgatcgg cgccacctgc gaggtggagc gcatcgacgg cgacggcgcg
    12781 gcctgggtgg cggtgtggtg gaacggcttc gacggcccgc tgctgacgac ggtggggctg
    12841 gatccggcgc agatggaaaa gatggcctga ggatggcctg aggtccgcgc gcgggcctgc
    12901 cgccgcgggc aggctcacgc ccggcgcgac ggggcgcggg agcggtttgc agcgtccccg
    12961 cgccggggcc gccggccctc actcgtcggc ggcggaggcg tcgtagttct tcaggcgctt
    13021 ctcggccgac acgccgagga tcttcagcag ccagggcggc ggcgattcac gcatggcctg
    13081 ctggaagcgg accaggacct cctcggcctt gccgccatcc ggtagcttga tggggtagat
    13141 gtcggcgcgg atcaccttgg cggcggcggg accgccgatg gacaccacgt aggcgacctg
    13201 gcagtcgccg atcagctggg cgcgccacag gttcttgtcc ggcgcgaact ctgcgtccat
    13261 ggtgtcgcgg atgtccacca ggcggatctc gtccggcgac acctgataga ccaggaagcg
    13321 caggcaggag ccgaagtggc cggacagggt gtcgccctgg tcggaggcga tggcgacccg
    13381 caccgagcca ggcatgtcgc cctcggtgta gggctggatg cgcggcaggg agtcgtcacc
    13441 ctgggtctcg ccccacagga tgcgcacggc cagcttcatg gcctccaggc cgatcccgat
    13501 gtcctcgccg tcctcttcgc cgtcggcgct ggcgaagccg gtcttgagct gggtgacggt
    13561 gaccttgcgc aggtcgtcga gctggatctc ttcgccgagc tggcgttgca ggagctcgat
    13621 caggcgcggc agggtggcgt tgggcatggc ccgggccgca agggcgatgc gcagggcaac
    13681 ttcccggctg atgggcgccg tggcagtttc catatttttc tcctctgtct gggctggggg
    13741 gtgccgggac gatgcaggtt cagtgccagc ggagaaagtg ccgttttttc aggggtttgg
    13801 aatggaggcg ccaacgtcct gggcgcccgc gtgtgtcgga taccccacac ggggtcggtc
    13861 acgccggtgc cgggtcgccg gcaaatacca tgcggccacg cccgcggtcc ttggcgacgt
    13921 acatggcgtc gtcggcggcc ttcatgaggg cgagatcgtc gacgccctgg tccgggtaga
    13981 gggcgatgcc gatggaggcc gacacggata gacggtgatc ggcgatgtag aagggctctt
    14041 ccacggtctg caggatgcgc tgggcaacga tgcgggcgtc gtcggcggcg tccacggggg
    14101 ccagcaggac cacgaactcg tcgcctccga ggcgtgcgag ggtgtcggat tcccgcaggc
    14161 acagcttcat gcggcgcgac acctcgcgca gcagatcgtc gccgacggcg tggccatagg
    14221 catcgtttac gggtttgaag cggtccagat ccacgaacat cagcgccgcc ctggtggtgt
    14281 cccgccgggc ccgcgcgagg acggactgga ggcggtcgga gaaaagggcg cggttgggca
    14341 gcagggtgag ggggtcgtgc tgggccatca ggcgcatcac ctcggcgttc tcgcgcagct
    14401 cctgggtcat gctgcgggcc tgggcatggg cgcgggtctg gcaggtgatg agctgccaga
    14461 tcagggccgc gatcagggcg ctcaggctga tgccggcgat gaggacgatc tcggcggcct
    14521 tgctgccgaa gcggcgttcg aattcgggct ggctgcgcac caccaggcac cagtcgtgac
    14581 cggctgcggc gacgaactcg cgcccttcga agcggaccgg cgccgctgcg tcctgcccgc
    14641 cgcggctgtc gtagaggagg gcgtcggggc ggggctcgat tccatcgtag agctgcacgt
    14701 cgagccccgg cggcgtctcg ccggcgaggc tggacatcag gtccttcatg cggaaggcgg
    14761 cctggaccca gccggtgagg gcgtcgcggc gttcggcctg ggtgttgagc ggtcggtcgt
    14821 ggcgatacag ggccaggtac aggataaagg cgctctccgg cccgtcctgg gtgtcggaat
    14881 gcaggcgcag gcgctggctg atggccaggg tgccggaatc ccgggcgtga agcagggctg
    14941 cccggcgggc cgcgtcgatc atctggtcat gcccgacggc gcgctggttg ccggtgaggt
    15001 ccggcgccgc atagatcatc ggggcgtgca ggccgtcggg gccgggcacc gcgcggctgt
    15061 aaaccaggct ttgcaggccg gcgaactcgg gcccctgagc caaggcatcc acataggtgg
    15121 cgaaatcttc gcggttgacc aggtcggagg cccggaagaa tccctgcacg ccgcgcagca
    15181 tctgggtata gacggcgatg cgctggtcga tgcgggcggc ggtctggcgc acgctggcgt
    15241 cgaagtgggc cgccatcgcc gcctggttgg cctgctgctc gtgctgccac agcaccagcg
    15301 tgagggccag ggcgccgaag ccggcgacca ggggcaggag gagttcgcgc aggcgctggg
    15361 cggcggtcat ctcagaaacg ggtcatggca cgggccagat ccggcttgaa gtatttgccg
    15421 aagatgcgtt cgacgctgcc gtcggcgcgc atgccgtcca ccagggcccg ccagcgggcc
    15481 tgttcctccg gcgacacggc cgcgcgggac atgatcaggc cgtgggggat gggcggatcc
    15541 gggaattcga gggtgcgggt ctgctcggcc aggcgcgccc gttccaggat cgggatgtcg
    15601 aagggttcga tgatcatggc ctggatgtgg tccgtcgcca gggcccggta cagggtgtcg
    15661 agggtgttgg cgtggatgac ccggccggtc tgggcgagac gatccaccag acggttggcg
    15721 gtcgggccgt agcggaaact gcggatcgcg cccaggcgca gcgcggggcg ggcgtccagt
    15781 tcgtcgatgc gcgtcacgcc ggcgtccttg cggaccagca gggtgtagcg gttggagaaa
    15841 taccaggcga aggcggcgaa gcgctcgcgg gcgtcattgg tgatgcccga caggctgaag
    15901 tcgagggcgc cggtctcgat gagctgccag atgcgggcgc gcgggagcac cgtcacattg
    15961 accgggcagc cgctgcggcg gatcagttcg tcggcgaagt ccttgtcgat gccttctccg
    16021 ctggcgccgg aatagagcag accatgctcg tgcagcgcca gggtacgcgg tcgcgtgcag
    16081 tccggcccgt cggcggcggc cggacgggtg aggcacagca tcaggcagac aaatgcgcag
    16141 tacagaagca tgaacggacg ggccggttcg ggtggagggg ccggcgcgta ctggaagcaa
    16201 cgggtgaccc aagccggcat tgtccggcag cgcggcgacg gcgacgaagg actattcatc
    16261 cgaaattttg cgatatttca catttcgatt cgatcaggcc gacgccgccg atccgcggcg
    16321 ccccgtccgt gtcgcatcag accgcggctt cctcggcggc gcgctcttcc tgctgcaggg
    16381 cccacatctg ggcataggcg ccgttgcggg cgagcaggct ggcgtggtcg ccccgttcga
    16441 cgatgtggcc ctggtcgagc accaggatct cgtcggcgtt catcaccgtg gacaggcgat
    16501 gggcgatgac cagcgtggtg cgcccccgcg cggcggcgtc gagctgggcc tggatggcct
    16561 tttcggtctt ggagtcgagg gccgaggtgg cctcgtcgaa gatcaggatg tccgggttct
    16621 tgagcagggt gcgggcaatg gcgacgcgct gcttctcgcc gcccgagagc ttgagtccgc
    16681 gctcgccgac gcgggtctcc cagccctcgg gcaggcgcgc gatgaagtcc tccagctggg
    16741 cggcacgggc ggcggcttcg atctccgcgc ggggggcgtc ggggcggccg tactggacgt
    16801 tgtagtagag ggtgtcgttg aacagcacgg tgtcctgggg aacgatgccg atggcggcgc
    16861 gcaggctgtc ctgggtgagg gcgcggatgt cctcgccgtt gatgcgcacg gcgccgccct
    16921 gcacgtcgta gaagcggaac agcaggcggg cgagggtgga cttgccggag ccggaatggc
    16981 ccaccaccgc caccgtgtgg ccggccggga tggcgaaatc cacctggtgg aggatggcgc
    17041 gggccggctc gtaggcgaac gccacatggt cgaagcgcac ctcggcgggg ccgcgggcca
    17101 gcgcgcgggc gtcggtccgg tcggccacct cgcggcgggc atcgagcagg ccgaacatgc
    17161 gctcgatgtc ggtgagggcc tggcggatct cccggtagag cacgccgagg aagttgaggg
    17221 ggacgtagag ctggatcagg aaggcattca ccagtaccag gtcgccgagg gtcatctgtc
    17281 cgcccgccac gcccagggcg gcgcgccaca tcatggccgt aacgcccacc gcgatgatcg
    17341 cggcctggcc catgttgagg agggacaggg actgctggtt gcggatctgg gcctcggtcc
    17401 agcgcgccag ctcggcgtcg tagcggcgcg tctcgaaggc ttcgttgttg aagtacttga
    17461 ccgtctcgaa gttgatcagg ctgtcgatgg cgcgggcatt ggcggcggaa tccagctcat
    17521 tgacccggcg gcgcagggcg gtgcgccagt gggtgacgcg caccgtgaag acgatgtagc
    17581 aggtgagggt ggcggcggtg atggcggcga agctgggttc gtacttggtc agcaggatgc
    17641 ccatcacgag gccgatctcc accagcgtgg gcaggatgga gtagagcgtg tagctgatca
    17701 gcgagccgat ggagcgggtg ccgcgctcga tgtcgcgggt caggccgccg gtctggcgct
    17761 ccaggtggaa gcgcagggac agggcgtgca ggtgatggaa gacctgaagg gagatctccc
    17821 gcaccgcctc ctgggtgacc cgcgcaaaga tgatctcgcg cagttcggtg aacagggagg
    17881 tggagaagcg cagcacgcca taggccagca gcagggcggc ggggaccacg atcagggcct
    17941 ggccgcggga caggtccagg tcgtccacca ggcgcttgaa gatcagcggc accgacacat
    18001 tggcgaactt ggcggagatc aggcagacca gggcgaacag cacgcggccc ttgtaggccc
    18061 acaggtaggg cagcagctgg cggatcgtct gccagtcatg gcggtcgccg gtggcggggg
    18121 caggaggcgg gccggagggg gagcgtcgca cggaaggtcc tcgggacgaa gggtgtgaag
    18181 tcggggtgat gggcgctcag gcgcccgctg agggttcggc cagcagacgc agggcgatgc
    18241 cggcaaagat caggccgcac aggcgatcca gccacgggcc gagggccggg tggcgggcca
    18301 gggcgtgccc caaggcaccg gaagcaaggg cgatgccacc gaagaccacg accgtctgcg
    18361 cgctgaacac caggcccagg acgatcatct ggcgtgcggt gtcgccacgg gccgggtcga
    18421 cgaactgggg caggaaggcc aggaagaaca gggccacctt gggattgagg gcgttggcga
    18481 cgaagccgcg ccgcagactg cgtcgccatg gcaccgccgc agcgtcgcct gcccgaacgg
    18541 ggcggctgcc gcgcagggcc tggacggcca gccccagcag ccacgcggct ccggccacgc
    18601 gaagggcgaa aaaggcccgg ggcgacgcgg ccagcagggc ggcgacaccg agcaccgtcc
    18661 acgtgaggtg ggtcagacac cccagggcgc agccgagggc gatggccagc cccgcacgcc
    18721 agccctgggc aagggcctgg ccgaggacca tcaggttgtc cggtccgggc gcgagggtga
    18781 gggcgatcga gaggccgagg aaggcgagca gggtgtcgaa gctgggcatg acccgccgat
    18841 tctcggcggc tggcgcggag gcgtcaatgg aacgcccgtc gcggcgacgg gcggttcccc
    18901 ggcgctagtg ggcgtgaccg tcgccggcgt ggcacagcac cgacgggtcg ccgacagccg
    18961 cctcgcccat gcgctgcagg tgatcgagca cgcgcaggga cgcctgctcc atgcggtcga
    19021 gctggcgcac caggtcgacc gtgttgccgg cggtcagggc gcgcaggacg tcggcgccgg
    19081 cccggtgcac ctcgatgtgg ggcggctcga cttcccgata gcccggaagc cggctgaagc
    19141 aggcctggcc ttcgcccgtg tagtaccact tgcccaggcg gcacgcggtg tggtcgggca
    19201 gggcgccgac gtccaggggc tgcaggccga acaccgccag gtagatgcgg aacttgaaga
    19261 cgatgtggtc gatcttggcc acctccacga agctcttcat ggcgctgccg gcgatgacgc
    19321 cctccatgtt gccggacagc gtcatcaggc gctgcatgtc ggccgtggcg agctgaccgc
    19381 gggcgctgaa ttcaccggcc gcgcgggaca ggctctccat ggcgctgcgg gcgcgggccg
    19441 agtcgtcgcg aatacggttc accagggccg agatctcgtt ggtggcggtg ctggtccgct
    19501 ccgccagctt gcgcacctcg tcggccacca ccgcaaagcc gcgcccggcc tccccggcgc
    19561 gggccgcctc gatggcggcg ttgagggcca gcaggttggt ctggtcggcg acgccgtgga
    19621 tgagcttgac gatggtgccg atttcgtcgg cgcgctgggc gagggtgtcc acctcccgcg
    19681 ccgactggga cgattcgtcc gccaggcgtt gcagattggc ggcgatctcg gtggtggcct
    19741 gaccgctcgc cagggacaca tgggccgcct ccacggcctt ctccttctcg tcgcgcagca
    19801 gccgcgccat ctcgcccagg ctgccctggg cgtccgtcag ggaggtgccg aactccgcca
    19861 tgcgcgtgat gacggcgcgc agcatggcgg cttcattgtc ctgtggggaa ggggccgcac
    19921 gggcggcaag ctgggcgcgc agggcgtcga tctcggcgtt cagcgcgcct tcctgacgct
    19981 gtgccccgtc gagccggcgc tgcatgtctt cgaatcggtg tcgggcgatc cacatagctg
    20041 tttttcctgg tgtatcggca ttccttcggg atgccggcga gcgatgtcct ctcgggctgg
    20101 atgtcggttc cgccgcgcat ttcttgaggt cagggacata aattgtttcc gttggccacg
    20161 gcaggcgcga caatcgaccc cggaccgatc cggtccgccg cggaggaatt tcatcatgaa
    20221 tggtcgcgac gaacgcctgc ccgccgcgct gctgggcctg gtcgcgctgg tcctggcggt
    20281 gtccgccatc gccccctacg accgggccac ctggtggatg gaggtggcgc cggtgttcct
    20341 ggtggcgccg ctgctgatcg ccagccaccg gcgtttcccg ctgacgccgc tgctccagac
    20401 gctgatcgcc ttacacgccc tggtgctcat cgtgggcggc gtgtggacct acgcgcgcgt
    20461 ccccttcggc ttctggctgc aggatgtctt tcacctggcg cgcaatccct atgaccgcat
    20521 cggccacttc atgcagggct tcgtgccggc cctggcggcg cgggaggtcc tgctgcggcg
    20581 cgggatcgtg cgcggacgcg gctggctgtt cttcctggtg acctgcgtgt gtctggccgt
    20641 ctcggccctc tacgaactca tcgaatgggg cgccgccgtg gccctcggcc agggggcgga
    20701 cgccttcctc ggcacccagg gcgatccctg ggacacccag aacgacatgg ccatggccct
    20761 cgtcggcgcc ctggtggcgc agatcggtct gggtggctgg cacgcgcgcc agatcgcgcg
    20821 cctgcgcctg tagcaaagcc gacaggcgcc tgcacacgcc gccccgatcg ccggactggc
    20881 gcccgcggcg gcgcgggatc gacgcttggt accgatcttg ctgagtcggg ttcgacctca
    20941 aaatattgtc gacctcaagc catgcccgac ctgcccgtca tgccgcccct tgaagccgct
    21001 cccgccgagc cccccgcggc gccgatcacg cccgccgacc tggtgctggc ctatcaccgg
    21061 cgcacccgtc accgcttcga cgcctacgcc cgcggcccct ccacactgga ttgggacgcc
    21121 cagccggcgc ccttccggca tttcggcggc acgcgccgca tccgcctgcc cctggcctgt
    21181 gccgaggcct gtgccgcgcc gcccgccacg ccggccgcag cggatctgac ggctctcggc
    21241 acgctgctgc acctgtcctt cggcatcacc gcgtggaagc gcctggggcc ggaccgctgg
    21301 gcggtgcgcg ccaacccgtc cagcggcaac ctgcatccgg tcgaagccta cgtgctggtg
    21361 gccggcctgc ccgacctgga ggacggcctc taccactacc tgccggatgt ccacgccctc
    21421 gagctgcggg ccgcccatcc gcgccccttc gcggccgggc cggcattggc cgtggggctg
    21481 accagcgtca tgtggcggga gatgtggaag tacggcgagc gggctttccg ctactgccag
    21541 ctcgacaccg gtcacgccct ggcctgcctg cactacgcgg cggccctgct gggctggcgg
    21601 gtgagcgacc ggcccggcct ggcgtcggcc gcccttgccc gtgtgctggg caccgaccgg
    21661 gacgaggact tccccggccg tcgccctgac gtgagccgcg aggaggtcga gctgctggcc
    21721 tgcgtcggtc cggcggacgg cctgccggtc gtcaccgccg acgccctcgc cgaggccgcg
    21781 gacgccgccc gctggcaggg cgtcgccagc gaggtggacc gtcaccccat gttccgctgg
    21841 ccggtggtgg acgaagtggc cgccgccagc cggcgcgtgt ccggtgtccc ggtgacgccg
    21901 ctaccggtgg cgatcccgcg gccggcatcg gcgcggcaca tcctgcagcg gcgcagcgcc
    21961 cagcgtttcg acccggccca cgtgatgccc cgcgaggact tcgtgcgcct cctcgacgcc
    22021 ctgcgtccgg tggcgggcgg gcgtctcggc ctggccctgt tcatccaccg ggtggacggc
    22081 ctcgcgcccg gcgtctatct gctggcccgc gccgactggc tgcggcccca gccgacccgc
    22141 gccggggcgt ccgcgctgca gcccgtgacc ggctgtccgg acctgtactg cctgtccacc
    22201 tcggaatccg tccccctgcg ccgtctggcg cgcagcctgc actgccacca ggacatcgcc
    22261 gccaacgcct gcatcgcctt cggtctgctg gcgcagctgg agcgggaggt cgccgacgat
    22321 ccggcccgct atcgcgacct gctgcgggaa gccggcttcc tcggccaggt gctctatctg
    22381 gaggccgagg ccatgggcct gcgcggcacc ggcgtcggct gctatttcga cgacccggtg
    22441 cacgaactgg ttatcgccgg ccagcgggat gcccactccc tctaccactt caccatcggc
    22501 ctgccggtgg acgacggccg catcgagacc ggccccgcct accccgccac gttggagatg
    22561 ccgtcatgaa cgcttccgcc accgcgcccc tgtccgccaa gttccagcgc atctccgccg
    22621 ccgaggccgc cgcgctgctg cgccgtccgg aaggggcgcc gcgtcccgtg ctcttcgacg
    22681 tgcgcgaccg ggccagctac gagcgtgccc acgtgagcgg cgccgagcat ctcaccgacg
    22741 cccatctggg cgccgccctg cgccgcgtgc cgaaaagcgc ggcggtgatg atctactgct
    22801 accacggcaa tgccagccag gtgtatgccg agaccttcgc cgatttccgc ttcgccgagg
    22861 tgtacagcgt cgatggcggc tacgagcccc tcgccgcggc actggccctc ctcgacggcg
    22921 tgcgcttggc gccgcgtctg ccggacgggg cgagcgccga cctgcgcgcc ttcgtcctcc
    22981 gccacgcctt cgatccggcc gatctggacg cgccgcgggc gcagggcctc accccgctga
    23041 tgcgcgccgc cctgcagggg caggccgaac tggtggggga actgctcgcc gcgggcgtgg
    23101 aggtggatcg gcgcaacggc gacggcaaca acgccctgtg gctggcctgc gtgtcgcggg
    23161 atgccgccac cgtggcggcc ctggtggccg ccggcatcga cctggacaac cgcaacgaca
    23221 tgggcgccac ctgcctcatg tacgccgcct ccagcggtcg ccccaccatg gtggaggcct
    23281 tgctgaaggc tggcgccgat ccctacgtgc gcaacttcga cgacgcccgc gcggtggacc
    23341 tgtgcgccac cctcgagtgt ctgcgtctgc tgcgtcacac gggcaactga gcgccacgcg
    23401 gcgccccccg accccggaga gagatcatga gcaaataccg ttcgattgcc ctgctgatgg
    23461 aagccgtcga ggcgcccctg gccgcggccg gcgtcgtggc cggcgaggcc tggcgtgccg
    23521 acgaacaggc ggtgggcttc ttcagtcacg ccgacgtggg cctggcggcg tatgtattca
    23581 cctacggcca gcgttccggg cgctacggga tcaacctgga cttcccgctg ccggcagaag
    23641 gcggtccgct cctcaccgtg cccaccagcg ccgaggatct gcccctcgcg gcggtcatcg
    23701 ccctgctgct ggagcacttc gacgtgccgg tgctggaagc ggcctgagcc ccagccggcc
    23761 ggacccggcc ggctcccgaa cgcgatcaat ggcgtccggc tgtgcgtgcc gcacgctgag
    23821 cgggcggaca ctccggccgc tccggcacga cgcccgctgt catgatgagg acggcgatcc
    23881 gcatgtgagg cgcgtccccc ggtcaccgcc gctggcgcgc cgtaccgacc gcggaccggg
    23941 ccgcccgcgg cgcctcagtg cagcgtgcgc ggcatcgcca gcgtgaattc cgggatcgtc
    24001 gcgttgaaat cgaccccgtc ctccgtctgc atccggtagc tgcccttcat cgtgcccacc
    24061 ggcgtggtga gcacgcagcc gctggtgtac tcgaacgcct cccccggctt gagggtcggc
    24121 tgctggccga tcaccccgct gccttccacc tcctggatcg tgccgtgggc atcggtgatg
    24181 atccagtgcc ggctgaggag ctgggcgggc gcgtcgcccg tattgcggat cgtgatgtga
    24241 taggcgaaca cgtagcggtc cgccgcctcg tcggactggt cggccagata gcgggcgacc
    24301 gccttgactt ggatggaatc cggatcgggg agcgtggtca tcgtcggcgc atcggttgcg
    24361 aggatgacgc attgaagccc atcgccctca tcgctgcaag tccggctttc cccaacgtgg
    24421 cgaacggtcc gacgggcggg tacactgcgc cgttttccgc gtctgccagg atcgaccatg
    24481 agcttccgca tcgcccccag cctgttgtcc gccaacttcg cccagcttgg cgcggaagtc
    24541 gccaacgtga tcgccgccgg tgccgactgg atccacttcg acgtgatgga caaccactac
    24601 gtccccaatc tcaccatcgg ccccctggtc tgcgaagcca tccgcccgtg caccaccgcg
    24661 cccatcgacg tgcatctgat ggtcaagccg gtggatcgca tcgtgccgga cttcgccaag
    24721 gccggcgccg acatcatcac cttccacccg gaggcctccg agcacatcga ccgtacgctg
    24781 ggcctgatcc gcgacagcgg ctgccaggcc ggcctggtct tcaacccggc cacgccgctg
    24841 cactggatgg atcacgtcat ggacaagctc gacgtggtgc tgctgatgag cgtcaacccg
    24901 ggcttcggcg gccagtcctt catccccggc accctcgaca agctgcgcga ggcgcgggcg
    24961 aagatcgacg cctacacggc ccgcagcggc cgccccatcc tgctggagat cgacggcggc
    25021 gtgaaggtgg acaacatcgc cgagatcgcc cgcgccgggg ctgacacctt cgtggccggc
    25081 tccgcggtct tcggcgccgg ccgcgacacc gacccgcacc gctacgacag cgtgatcggc
    25141 gccctgcgcg gcgccctggc gggggtctga ggtgtccccg cgccacgcgg tgctgttcga
    25201 tctggacggc accctgctcg attccatccc cgacctggcc gaagcctgcc agcgcatgat
    25261 gcgggagctg ggccatccgc cccacgacct ggagacgatc cgcagcttcg tcggcaaggg
    25321 catggtcaac ctcgtcaccc gctgtctcgg cgacgcctcc gcggaagcgg tggacgccgc
    25381 cgtggcggtc ttccgccgcc actacgccgc ggtcaatggc gacgccacca ccctctatcc
    25441 gggggtggtg ccggccctgg aggccctcgc cgcgcggggc gtgcccctcg cctgcgtcac
    25501 caacaagccg gccgccttca ccgagcccct gctggcgcgc tgcggcattg ccgattactt
    25561 tgccgtcacc gtcagtggcg acaccctcgc cgagaagaag ccccatcccg cccccttgca
    25621 gcacgcctgt cgcctgctcg gcgtggcccc cgcggcggcc ctgatggtgg gcgactccgc
    25681 caacgacgcc gaagctgccc gcgctgccgg catgccggtg ctcctcgtca cctacggcta
    25741 cagtgaggga cgcccggtgg acagcatcga ctgcgacggg ctactatcca gcctcgagga
    25801 cgtgctgagg cacctccccg cctgaaatcc cccggaattt catccccttc atgacccgta
    25861 agcgcatgtt cttcgtcgag gagttccctg ccgtccgtat cccccgctcc ggcggatgcg
    25921 gcggccggtc atggctgcgc gcctgacgcc gccctcccgt cgcgggccaa gccccgtgat
    25981 accctcgcga acacgcagca tgcccttccg gagtctccat gaccgaagcc gaattcaacg
    26041 ccctggccgc ccagggctat aaccgcatcc cggtaaccgt cgacaccttc gcggatctcg
    26101 acacccccct ctccgtctac ctcaagctcg ccaacgcgcc caacacctac ctgctcgaat
    26161 ccgtgcaggg gggcgagcgt ttcgggcgct attccatcat cggcctggcc tgctccacgc
    26221 gcatcgaggc gcgcggccgc agtgtgatgc ttctcaccaa cgaccgcgtc gtggagcgtc
    26281 gcgacgacac caatcccctc gattacgtgg ccgaattcaa ggcccgcatc aaggtgcccc
    26341 cgcgcgaccg cctgccgcgc ttcgccggtg gcctggtcgg ctgcttcggt tatgacacgg
    26401 tgcgctacat cgagccgcgc ctggccagca cccacaagcc cgacgccctc ggcacgcccg
    26461 acgtgctgct gctcctgtcc gaagagatcg ccatcgtgga caacctgtcc ggcaagctca
    26521 ccctcgtggt gtatgccgag ccccaggtgc ccgatgccta cgcccgcgcc acccggcggc
    26581 tcaaggaact catcgggcgc ctgcgcgagc ccgtgtcgat tcccgccgag gcgcccggcg
    26641 cccccgccga ggccgtctcc agcttcggcg aggcggcctt caaggacgcc gtgcgcaagg
    26701 ccaagcacta catcgtcgag ggcgacatca tgcaggtggt gctcgcccag cgcatgagca
    26761 agcccttcgc cgccagtccg ctggccctgt accgggcgat ccgcagcctc aacccgtcgc
    26821 cctacatgtt ctacttcaac ttcgaggact tccaggtcgt cggcgcctcg cccgagatcc
    26881 tcgtgcggct ggaggacaac gtggtcaccg tgcggcccat cgccggcacc cgcaagcgcg
    26941 gcgccaccca cgacgaagac ctggccctgg aggccgaact cctcgccgac gagaaggagc
    27001 gggccgagca cctgcagctc ctcgacctgg gccgcaacga ctgcggccgc gtggccggca
    27061 ccggctccgt gcgcgtcacc gagcagttca ccgtcgagcg ctactcccac gtgatgcaca
    27121 tcgtctccaa cgtggaaggc cgcctgcgcg acgggctcga cgccctcgcc gtgctgcgcg
    27181 ccaccttccc ggccggcacc gtctcgggtg cgcccaagct gcgggcgatg gagatcatcg
    27241 acgagctgga acccaccaag cgcggcatct acgccggttc ggtgggctat gtgggcttcc
    27301 acggcgacat ggacctggcc atcgccatcc gcaccgcggt gctcaaggac ggccacatcc
    27361 atgtccaggc gggtgccggc atcgtcgccg actccaaccc cgacgccgaa tgggaagaga
    27421 cccagaacaa ggcccgcgcc atgctgcgcg ccgccgagat cgccgaagcc ggcctcgaca
    27481 cgccgctgtg aacgcccggc aaaaggccgc ccgcaggcgg cctgaagcgc tgcaccggca
    27541 cgtccggacc gcgcccgccc atgtcggtgg ggcggcggtc cggaggcgcc gcgcgtcaga
    27601 aggcgatgcc caccttggcc tgcaccagcc agttctcggc gccctgcatg ccgggggcga
    27661 tgtccttgtt caccgtgtcc tgccagcgcg cgccgaggcc cagattcacc tggccgatgc
    27721 ggtaatccag gtctgcgccg gcctgccagg cgaacaggtc ggtggtgcga ccgcccgcgt
    27781 ccgccttcac gtagccgatg ccggggccga cgccgaaggt caggttctgg gccaccggga
    27841 tcgtccagcg cgggttcagt tccacggtgt tcagggtcac gccgtccttg tcatagcggt
    27901 tccacgacag cttggtgcgg accacgccgg tgggcgtctg gaacaggccg cagttcatgg
    27961 ccagctccag gcccagggcg gtgtcgctgc tgccgtgctt ggggtcgagc atgccgccgg
    28021 tcaccgacgc gacgaagttg ggccggtagt ccttgtccag gatgggcagg aacttccagt
    28081 caccggcaaa ggccggggcg gtggcggccg ccagggtgac ggcggtcagc aggatgcggg
    28141 cgttcttcat ggttcgtcct cttggtgttg tggaaaatgg atccatcatg accgatggcg
    28201 ccgctcgaag tcggtaaccc cggtcacata gccatttttc cgtcccgcgc cccctgcgac
    28261 gggtcggaac tgcgagaaaa tgtttcagcg tcgcgccccc gcgggtcgcg gcgattgccg
    28321 aaaggaagtc cagccatgct gctgatgatc gacaactacg acagcttcac ttacaacctc
    28381 gtgcagtact tcggcgagct cggtgccgac gtgcgcgtgt tccgcaacga cgagatcacc
    28441 gtcgagcaga tcgccgaact ggcgccggcc cagatcgtcg tctcccccgg cccctgttcg
    28501 ccggccgagg cgggcatctc cgtcgccgcc atccagacct tcgccggcaa aatcccgctc
    28561 ctcggcgtgt gcctcggcca ccagagcatc ggcgccgcct tcggcggacg catcgtgcac
    28621 gccaagcagc tcatgcacgg caaggtgtcg ccggtccatc acctggacca gggcgtcttc
    28681 aagggcctgc ccaatcccct cacctgcacc cgttaccact ccctcgccat cgaacgcgag
    28741 accctgcccg actgcctcga agtcaccgcg tggaccgacg acggcgagat catgggcgtg
    28801 cgccacaaga ccctcgcggt ggagggcgtg cagttccacc ccgagtccat cctcaccgag
    28861 cgcggccacg acatgctgcg caacttcctc gacgcatccc gctgaacgga gcccgccatg
    28921 aacttcacgc cccaggaagc cctgcaacgg accatcgaac accgggagat cttctacgac
    28981 gagatgctct ccctgatgcg ccagatcatg gcgggcgagg tctcgccggt gatgaccgcc
    29041 gccatcctca ccggcctgcg ggtcaagaag gagaccatcg gcgagatcgc cgccgccgcc
    29101 acggtgatgc gcgagctctc ctccaaggtc atcgtgccgc cgccccacga gcacttcctg
    29161 gacgtggtcg gcaccggcgg tgacggcgcc cacaccttca acatctccac cgccaccatc
    29221 tttgtcgccg cggcggccgg cgcgcgggtg gccaagcacg gcgggcgcag cgtctccagc
    29281 aagtccggca gcgccgacgt gctcgaagcc ctcggcgtca acctcaacct caacgccgaa
    29341 caggtcgccg aatgcatcgc ccgcaccggc atcggcttca tgttcgcccc caaccaccac
    29401 agcgccatga agaacgtggc gccggtgcgc cgcgagatgg gcgtgcgcac cctcttcaac
    29461 atcctcggcc ccctcaccaa cccggccggc gcgccgaaca ccctgctggg cgtcttccac
    29521 gccgacctgg tgggcatctg cgtgcgcgtc atgcagcgcc tcggcgccca tcacgtgctg
    29581 gtggtgcatg ggcgggacgg catggacgaa gtctccctcg gcgccgccac cctggtgggc
    29641 gagctcaagg acggcgagat ccgcgaatac gaaatccacc cggaggactt cggcctcgcc
    29701 atgatgtcca gccgccgcct gcaggtgagc accgccgacg aatccagggc catgctcctg
    29761 tccgtcctcg acaacgtccc gtccgccgcc cgtgagatcg tcatcctcaa cgccggcgtc
    29821 gccctctaca ccgccaacct ggccgactcc atcggcgccg gcatcgccct cgcccgcgag
    29881 gccatcgcct ccggcgcggc ccgggccagg ctcgacgcct ttgtcgccgc cacccgttcc
    29941 ttcgcctgag cgtcccatgt ccgacatcct caacaagatc ctcgccgtca aacgcgacga
    30001 agtcgccgcc gcccgcgccc gcgtctccga tgccgccgtg cgccagctcg ccgccgacca
    30061 gcccgccccg cgggatttcg tcgggtccat gcgtgcccgc ctgggcgccg gccgttccgc
    30121 cgtgatcgcc gagatcaaga aggccagccc cagcaagggc gtcatccgcg ccgacttccg
    30181 ccccgccgag atcgccccgt cctacgagcg cgccggcgcc gcctgcctct ccgtgctcac
    30241 cgaccgccag tttttccagg gcgcgccgga ctacctccag gccgcccgcg ccgcctgcac
    30301 cctgccggtg ctgcgcaagg acttcctcgt cgatccctac caggtcttcg aggcccgcgc
    30361 gatgggcgcc gacgccatcc tcctcatcgt ctccgccctc agcctcgccg agatgcaggc
    30421 catggaggcc atcgccgaat ccctcggcat ggccgtgctg gtggaagtcc acgacggcgc
    30481 cgagctcgac ctggccctgc agctgcgcac ccccctggtg ggcatcaaca accgcaacct
    30541 gcgcaccttc gaagtcagcc tgcagaccac cctcgacctg ctcccgcgca tccccgccga
    30601 gcgcatcgtc gtcaccgaat ccggcatcct gcagcccgcc gacgtggccc tgatgcgcga
    30661 gcaccgggtc aatgccttcc tcgtcggcga agccttcatg cgcgccgacg atccgggcgc
    30721 cggtctcgcc gccctgttcg cctgatccgc catgggcgac gccgcgccgc cgcccgaccc
    30781 cgccaccccg ccgcccgcgc aggccccgtc gcccgcgcgg cgtggggctg cggatgcttt
    30841 cgcccgcctg ctgcgggcca cgctgggggg cgcgttcgcc acgctcgccc tcctcatcgc
    30901 cacgctgggc gggctgggcg gcaccgaggc cgggcttagg ctgctggctg aaggggccac
    30961 cgccctcagc ggcggcgatc tgcgcatcga aggcgccgaa ggccatctcc tcggccacgt
    31021 ccgcctcgcc gccctgcgcc tgcatcgcgc cgatctccat ctggacctca cccgggtgga
    31081 tctggactgg acgcccgccg cgctgctcca cggccgtctc gacatcgccg ccctgagcgc
    31141 cgccgacgtc gtcgtcgcca cgcctccgga cgacaccccg gcgaccctgc cggccagcct
    31201 cgaactcccc gtcgctgtgc acgtcggccg cctgcacctg gaccgcctgc gcatcggcgc
    31261 cctccgcgac ggcgcagccg ctgcgccgga cgtggacctc acggaggtgg aggccgccgc
    31321 cgactccgac ggccgcctcc accgcgtcgc cactgcccgc gccaggaccg ccttcggcgc
    31381 gctcgccgcc cagggccagc tcgacggccg ccgccccttc gccctccgcg cccacgccga
    31441 tctggccacc cgccagggcg gcgacgccta caccgtgcag gccgatgccc acggcgacct
    31501 gcagaccctc accatcgacg cccgcgccgc cggtgccggc ctcagcggcg aggcccacgt
    31561 ggccgccacg cccttcgccg ctgtgccctt ccgcagcgcc cgcctggcga tccgcgacct
    31621 tgaccccgcc cgcttccacg cggccgcccc cggcgcccgc ctcgacgtga gcgccgatct
    31681 cgagcccgtc gccagcgcca ccgatggcgc gtggagcgtc gccggtccgg tgcgcctcct
    31741 caaccgcgcg cccggccgat acgacgccgg ccgcctgcct gtcgcgtccc tcgctgccct
    31801 gctgcgctgg ggcgaaggcc gtctcagcct gaccgacctg gacctgcgcc tgccccgcgg
    31861 cgaagtccgc ggtggcgcca gctgggtgcg cgccgcggcc ggggccccgg acggtgacat
    31921 cggccgtgtg gaagccgacc tgcaggtgcg cggcgtcgcc ctcccggccc tggacgggcg
    31981 cctgccggcc gccaccctcg ccggtcgcgt gcaggcccgc gccgacgccg gcgggcagac
    32041 cgtggaggcg gatctgcgcg atccgcgcgt ggccctgcgc ctctccgccc gccatgccga
    32101 cggcgcggtc acggtgcagg agctcctcgc ccgcgccggc aaggcccgcc tcagcgtccg
    32161 cggcgcggcc gacctggccg gcgacggccg cttcgagctc aagggccgcc tcgatcactt
    32221 cgatccccac gccttcttca cggaggcgcc cgccgccgac ctcaccctgg acctggaggc
    32281 ccgcggccgg cgcagcccgc agatggccgc aacgctgagc ttccagctgg cccccagcac
    32341 gctggagggc cggcccctcg tcggccacgg caccctcgcc gtggacggcc ggcgcctcag
    32401 cgacgccgac ctgacgcttg ccgtagccgc caaccgcctg tccgcccgtg gccgctacgg
    32461 tgccccgggc gatgccctcg acctgcacct ggaggccccc gatctcgccg ccctcgggca
    32521 caccctcagc ggccgcgtca cggccgctgc cgtgctgcgc ggcacgcccg cccgcccggc
    32581 cggcgagctg gagctgagcg gcgaaggcct cgcctatggc accgtgcatg tggagggcgt
    32641 caatgggcgg ggccggctgg aggagggcgc cgatgcgccg ctgaacctgc tcctcaacct
    32701 ggccggtgtg cgcacgggcg cggacctgga gcatggcctg cgccgcgcca gtctggtcgt
    32761 cgatggccgg cgcagtgccc accgcgccca cctggaagcc cacacgcccg gcgcggatgc
    32821 ccttgagctg gagctctccg gcggcctggg cgacgccctg cgctggcagg gacgcctcga
    32881 ccggctgcgc gtcctcgggc cccatgcgac gacgctggca gcgccggtgc cgctggtgct
    32941 ggccccggcc gaagtgcgca tcggggcggc cgaactgcgc agcgaggccg gcgccgccct
    33001 gcgtcttgac gagacccgct gggcggatgg cgagatcgtc gcccgcggcc ggctgtccgg
    33061 gctgccggtc ggcatcggcc tcgacgccgc cggcgcgaac cgcgccgcgc tgggcgacac
    33121 tctgcgcatg ggcggcgact gggacatcca cgtgggcggc cacgtggacg gcaccgtgca
    33181 tctcttccgg gagcggggcg acctgctggt gctgggcgac gcgccggtac gcctggggct
    33241 ggatgaagtg cagatcgtcg tcaacgcggt ggccagtcgc ctggccttcg gcttctcggt
    33301 gagcggcgcg cagctgggca aggcctccgg cgccggcacc gccctggccg agcgcacggc
    33361 cgacggcggc gtgcgcctgg tgcctgacgc gccgctgctg ggctcggccc agctggacat
    33421 gcccagcatc gcgtggaccg gccccctggc cgaccccaac ctcaagaccg ccggccgcct
    33481 gaaggccgac ttcaccctgt ccggcacgcc cgcccagccc gtcaccgaag ggcgcatccg
    33541 cggcgaggcg ctggccttcg cgatggccga ccagggcctc aacctgtccg gcggtaccct
    33601 ggtggccgac ttcaaccggg accgcctgcg cgtggagcgc ttcgacttcg cgtcgcccaa
    33661 ccgggtcaaa cccaacgaag ggcgcatcga cgtggcgcgc ctcacccgca cccccggcag
    33721 tctgcaggtc aagggcgaag tcgccctggc gagcggcgcc ggcgccttca gcttccaggc
    33781 cgagcgcctg cccctgctgc aacgccccga ccaatggctg gtgctcagcg gtgcgggcga
    33841 ggtgcacagc ggctggaatt cggcgcgcat ccgcggccgg ctcaccgccg acgccggcta
    33901 cgtggagctc tcccgcacac cggcgcccag cctgggggac gacgtggagg tggtgcgccc
    33961 cgaacaggcc cgcccgccgg cggtcaagcc cttcagcacc gcggtggacc tggccatcgg
    34021 cctgggggac gcgctctacg tgcgcgccct cggcctcgac acccgtctgg cgggcgagct
    34081 gcgcctgcgc gccggcgacg gccagcccct cgccatgacc ggcgtcatca acacccaggg
    34141 cggcgtgtac gaaggctacg gccagaagct ggacatcgag cggggcatcg tcaccttcac
    34201 cggtgcggtg agcaatccca gcctcaatgt ggtggccctg cgcaaaggcc tctcggtgga
    34261 agccggagtc gccatcaccg gcacggcgcg gcgcccgctg gtgcgcctgg tctccgaacc
    34321 caatgtgccc gacccggaaa agctcgcctg gatcgtcctc gggcgtccgc ctgaccaggg
    34381 ctccggcggc gagctggccc tgctgctgcc cgccgcccag gccctgctgg gcaccgacag
    34441 catgtcctcc ggtctggtcc gtgccctcgg cttcgacgag ttctcctttg gcagcagcgt
    34501 ggacagccgc cagcgcatcc agaccagcag cgttgccacg ggcagcgtca ccggcaccac
    34561 ctcgtcggcg ggcaccacca ccggcagcga atccaccgcc ggtcaggtgg tgagcatcgg
    34621 caagcgcctg tcggcccgcg ccctgctgtc cttcgagcag agcgtcaccg gcgcctccag
    34681 catcgtcaag ctgagctacc agctcacccg gcgcatcgcc gtggtgggcc gcgccggcag
    34741 cgagaacgcc ctcgacgcgg tgttcaccct ctccttccgc tgaagcctgc ggggccgcgc
    34801 caaggcggcg gcgggcgctt gatgcaggtc atggacggcc cggcccgtct cgggcgagat
    34861 gcggggcctg caccccggag gcccgcccat gaccgaaccg ctccagccct gccgcatccc
    34921 cgtctccgcc gtctgggcgg ccctggccga aggcgggcgg gactttcgcc gccatccggg
    34981 gccggcggcg ctcttcgcca gtgtgttcgc cgccctcggg ctggggctct tcggggtact
    35041 ggccgcctac gatctgcacg cccttcgcct gcccctggcc ggtggcttcc tgctggtggg
    35101 gccggcgctg ctggcgggct tcttcgccgt gcgccgggtg gccgcgaccc gcccgccgcg
    35161 gctggcggat ctgctcggcg gcttcgcggc ggcgccgcgg ggtgtgtggg cccttgcggc
    35221 ggtgtgcgcc ctgctgttcc tgatctggat gagcgacgcc gccaccgtct atagcttcat
    35281 gatcggcgac acgcggccgg gggccgacca ggtgtggcgc ttccacgcgt ggaccagcct
    35341 gatgggcggc gtcttcgcgc tcatcgtctt ccacatcacc gcctttgcgg tgcccctgct
    35401 cctcgaccgg cgcgccacgc tggtgggcgc ggtggtggcc agcgtgcgcg gcatctggac
    35461 caacgggccg gccatggcga cgtgggccgg cgtgctggcc gtggcgatca tcgccaccgt
    35521 ctttgtgccg cccctgctgg gcgtgtcgct gccctggctc gcctttgtca gcgatctcct
    35581 ttatcttcgc gtctacccgt cacgcccaaa atgagcagta cgctcctgga gaccgaccat
    35641 gaccgacacg acctccgaaa ccgccatcct ggccggcggc tgcttctggt gcctggaagc
    35701 ggtcttcctg ctcgccgaag gggtgcacac cgtcacctcc ggctacatcg gcggccatgt
    35761 ggatgcgccc acctatcacc aggtctgcga cggcaccacc ggccacgccg aagccatccg
    35821 gatcgaattc gaccccgcgc gcatcacctt cgacgacctc ctcgacatct tcttcgccat
    35881 tcacgacccc accaccctca accgccaggg taacgacgtg ggcacccagt atcgctccgc
    35941 catctttgcc accaccccgg cccaggccgc ggcggcccag gcccggatcg ccgccctggc
    36001 gggcgcgtat cacgccccca tcgtcaccga agtcgtgccc gcgccggtct tctggccggc
    36061 ggagccctat caccacgatt atttttcccg caacggccac cagccctatt gccaggtcat
    36121 cgtcgccccc aaggtgcaga agtttctgcg cgcgttcccg cagcgggtgc ggtcgacttg
    36181 atctagaatc cgggcggtct ttccatagga gcactgtatg tctgaagtca ccacggcgag
    36241 cggtctcgtc atcgaggatc tggaagtcgg caccggcccc gaagccagcg ccggccagcg
    36301 cgtgcaggtt cattacacgg gctggctcac cgacggccgc aagttcgatt ccagcaagga
    36361 ccgcaacgac cccttcgtct tccccctcgg cgcccgccac gtgatcgccg gctgggacga
    36421 aggcgtgcaa ggcatgaagg tcggcggccg tcgcaagctc accatcccgc ccaacctggg
    36481 ctacggcgcc cgcggcgccg gcggtgtgat tccccccaac gccaccctgg tgttcgaggt
    36541 ggagctgctg gccgtggcct gatcgcccgc ccgcaacccc ggccgtgacg aggccgaagc
    36601 gcgatggatg tcgcgcttcg gcctttttca tggggatgtc cgacagcggc ccggcggcgc
    36661 gggccgtctg cccctcaata tcggacggtc agcgccacat acacggagcg gcccatgccg
    36721 gggacggcga tgccgtaggg ggcgcccgcg ccgttgaggg acatggtggt gccctggccg
    36781 atataggcgc cgccgagggg cgaggcgtag aacctgttca gtgcattctc gaggcccaga
    36841 tccacccggt agtggcgcgt gtcgtagctg ctgcgcaggt tgagcagagc gtagccgccg
    36901 gtggcgatct cattgcgcac ctgggacacg gcgcgcttgg cgcccaccag cacttcctcc
    36961 accgcggtgg tccacgggcc gtgcttctgc tccaggctca ggcgggcgtt gaggggcatc
    37021 tggttgtaga gcccgtcgtc gctgtgggtg tccttgccat gcacgtacgc cagggtgccg
    37081 cggccggtga tcgggcccag ggtctcgccg ccgtccagca cccggtagcc ggacaggtcg
    37141 gcgccgtaca gccgcgcgtc ggtattgacg aggcgcaggt agttgaactg gttaggcggg
    37201 caactcgtca ggcagcgagc gttgatgtag ttgttcacat aagtgtagaa ggattgcagc
    37261 ttcacccccc agcgggcctg cccggcatcg tgcaggtcgg cgctggcact cagggtgtgg
    37321 gcgacttccg gcgccagatc gatgctgccc acgtagccgt tgccgtcgtt cacccagttg
    37381 ttcatggtca tggccatgcc gttggtggac caggtgtagc gttcatacag gctgggcgag
    37441 cgggtcttgc gggcgtagcc ggcatcgaaa ctgcggcccg catccggcgt gaagcgggcc
    37501 agcagggtgg cgtccaggtt gtggtcggta cgggcgcggc tggccgcatt gaaacgggcg
    37561 gcgtcggcgc cgtacatcat tgccatggtg ttgtagccct gcaccgggtc ggcatccatg
    37621 cgcacggtgc tgctgcgcag gccggccagg gtctgccagc ggggcgacca ggcggcttcc
    37681 cactcggcaa atacatccat gcggtcgcgc tggccgttgt ggagattctg gaaggtgttg
    37741 ggcgacatca tcatggcgtt cgccaccggc ggccaccagt cgttcaggtg gtagcgctgg
    37801 tattcgctgc cgacgcgcag aatatcccgc tcgttgaggt ttacatccgc gcggacgcgg
    37861 gcaccggtat tcacgccatg ggtctccatg ggcatgccgg ccacgttgcg ggcgctgcca
    37921 taccagtaca gtttgtcgtc gccgaaatcc atccggtggc gcgtttcctc gttgtaaagc
    37981 cgcgcctgca ggctgcccca ggcaaagctg ccggtgtagc ccaggttgac ctgggtgctg
    38041 tggttgtccg tcatgtccat gtgctggttg gggaagccct ggcggggaat ctcctgcacg
    38101 ctcaccttca ggtcggccat gtgctcgccg ctgcgcaggg cgaagcccag gctgtggctg
    38161 cgcagggtgt acgccgtcga gccgatctcg ttggcgtcga gggggtgggt accgctgcgg
    38221 gtccacgtag cctgggtggc gggcttgaag tccttggcgg cacgcaggtt gtccgaggtg
    38281 ctcacgcccc ccgcatagcg cacgctgagt cgctcgctgg cccagtgggc ctcgccctgc
    38341 acgccgcggg ctgcgccgtt ggaacggtag aaggcgccca gcgcgccgcc cgccacggtg
    38401 ccttggcccg gtgcggcgaa gggcggcggt gccgattccg ccaccacggt ggcgccgatg
    38461 ctgtcgccgc ccaggctcac cggtaccgcg ccggtaaaaa cctggatcgc ccccacacgg
    38521 aaggactcca ggtaggacag ggggggattc atgtgattgc cgcaggcgga gatcaggtcc
    38581 atgccatcca cctgcacccg gttgcggtca tccggcaggc cgcgcaccac cgggagactg
    38641 gagagaccgc cggcgccggc gagggcgaca cccggcacgt cctgcaacag ggcggccgtg
    38701 tcgctggtgg tggccttcag cccggccagc cgcgcggacg tgggccccgc cccctccgcg
    38761 aggggacgca aacgggtggc cttgacctcc acctcgggca gagcggcggg gccttctgcc
    38821 gcgcgtgccg gcacggtcgc cagcagggcg ggacaggacg aaagaaggag cagggcgagc
    38881 cggcggggct gaaaaggcag ggcggtcatg gcagatccga agcaggtcag ggacgaagcc
    38941 gaaattgtcc cggatcgcct gggcgcaccc ctgcggccta tcgtcgcatc gacggcgcct
    39001 tgccgaggct gcgcgggcgc ctgcccagca ggtctgccct cacgtgcgcc ccgtgagaca
    39061 gtgtcgaacc cacaaccggt atggtgtgtg gcccgtcgcc gggtccgccc ccctcgcgcc
    39121 gcgtgccggc tgccacgtac gcctcggccg gcggtgactg ccacacgcgg ccacccccgc
    39181 acctgcgacc gttccggacg cgttcccggc ccgccgtctt gccggacggc gagcgcggca
    39241 gaccggtgat cccgaacccg gagcagcccg tctcgctatt gaccgggatg gggctttacc
    39301 gggcctgaca cacacacgat tggacactca cgggggccgc ctctccactc ggtgcgtgtc
    39361 gaccctcgat tgcctcaaaa agaggcacgc gtggcttcca gagactcatg attgcctttt
    39421 tatgaggcga cgctgcctaa aaaataggca atgccgccta aaaaagaggc agcgttgcct
    39481 aaaatttagg caatcgtgcc taaaaaatag gcaatcatgg gtctcgaaaa cccactgttg
    39541 ccaaaaaaat aggcagtgct gcctaaaaat gaggcaatcg tgggattcag aaagcctcgc
    39601 gtgcctaaaa aataggcaat cgtccctccg cggtcgtcat gcatgccggt cgggaagcct
    39661 tgcgtgacgt gaagccaggg gcgcgtggca ggggcgacgc cgacagaggc gtggcg
//
LOCUS       DAOQMM010000020        39239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_257_length_39239_cov_44.823499, whole genome shotgun sequence.
ACCESSION   DAOQMM010000020 DAOQMM010000000
VERSION     DAOQMM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 39239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39239
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_39239_cov_44.823499"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLH85_05350"
     CDS             <1..189
                     /locus_tag="PLH85_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HNB64018.1"
                     /translation="ALFTSLMAVPRTFINASAGWLVEDLGWFRFFLLCGLLAVPGMLL
                     LFRVAPWRLADDSSEGMK"
     gene            186..776
                     /locus_tag="PLH85_05355"
     CDS             186..776
                     /locus_tag="PLH85_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNB64019.1"
                     /translation="MMGKGRLEAFSDGVLAIIITIMVLELKVPHGDDLAALAPLLPVF
                     LSYLLSFVYVGIYWNNHHHLLHAAHGVTGAVLWANLHLLFWLSLIPFVTGWMGENHFS
                     ALPTALYGGVLLMAAIAYFILQWAILRGDGTASVLAQALGRDWKGKLSPVIYIVAIPA
                     AFFAEWVSDALYVLVALIWLVPDRRIERALVVDEHP"
     gene            806..1519
                     /locus_tag="PLH85_05360"
     CDS             806..1519
                     /locus_tag="PLH85_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB64020.1"
                     /translation="MTALEAAARELSAALAPMRFAAPVSHVYNPLAYAWAPYADYLRR
                     FGQGRKRVVLVGMNPGPFGMVQTGVPFGEVAAVRDWMGIRAEVTAPAAQNPKRPVEGF
                     ACTRSEVSGRRLWGLFAERFGPAEAFFAEHFVANYCPLAFFDGGRNLTPDKLPAAEQG
                     PLLAACDAHLRRVAEILQPEWVIGVGAWAELRAREALAGLPLRFGRVLHPSPASPAAN
                     RGWAEAATRQLVDLGVWPA"
     gene            complement(1527..3011)
                     /locus_tag="PLH85_05365"
     CDS             complement(1527..3011)
                     /locus_tag="PLH85_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNB64021.1"
                     /translation="MRTLENLPFDNAFARLPEQFYTRLAPDPLPEPVLVAASPAAAAL
                     IDLDPAEFARPEFARVFAGNALLPGSEPLAAVYSGHQFGMWAGQLGDGRAHLLGEVPG
                     AGAGWEIQLKGAGQTPYSRFADGRAVLRSSLREFLCSEAMAALGIPTTRALCVVGSPL
                     PVRRETVETAAVCTRLAPSFLRFGSFEHWAVNKQPDALRVLADYVIDRFRPELRDAAN
                     PYDALLRDAGRRTGDLIARWQTVGFMHGVMNTDNMSILGLTLDYGPFGFMDGFDANHI
                     CNHSDDHGRYAYKAQPQIGHWNLFALGRALRSLVGSQDDVKAAIADSYVPAFENGYLN
                     GLRAKLGLQDALPGDEDFIGETLSLLQRFHPDYTTFFRVLSRLPGTVADASRATVDAP
                     LRDHFIDRDACDAWLTLWRARLAAEGSRDDVRQAAMLTVNPKYVLRNWVAEEAIRAAQ
                     QGDDRITAEVLACLSRPFDEQPEFERYAAPPPDWAQHLAVSCSS"
     gene            3160..4584
                     /locus_tag="PLH85_05370"
     CDS             3160..4584
                     /locus_tag="PLH85_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB64022.1"
                     /translation="MLQTHIDAPADAGRPAAPLPDSGVDLKRYLLWRVSLFALAIGLA
                     AVAAVFVQTGERLRGHIARTGSTVENLLAAEVDRPRDSFRRTVEGLDLTSLDGIGPLL
                     GICVKVEDIYKRAVIRRCFDEGEGAPAPVRAVLAALLGPDVAYRGVIGQYPGVKVGEF
                     TVTPDLDAAARDAWSRIGMVLGMTFGILLLNGLVVLPVRRALAPAEQILAVLARMEAG
                     DLAARMPRPRLIELRRIAAGFDHLVERLEATLAARRQLAQRLLAVREEERRHLARELH
                     DEFGQCLASVSAEAAFIGARAERSDPSLLPAARAVSAVTAHMMESLQGILHQLRPVGL
                     EEFGLAAALEQLVGGWRRRQPDCQFHLEMRGAVDDLPDELTVSLYRIVQESLTNALRH
                     GAPRRVAVSLVRDADGCRVTVDNDGQGRAEPNPGSGLGVLGMRERVEALGGQFDLLPL
                     TPQGMRVAAVLPVAALIPVETSHA"
     gene            4577..5212
                     /locus_tag="PLH85_05375"
     CDS             4577..5212
                     /locus_tag="PLH85_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB64023.1"
                     /translation="MLEPTRLLLVDDHAVVREGYRRLLETRAELRIVGEAATARDALD
                     AFRRLAPDVLVLDLGLPDMGGVELIRRLLQREPGARILVFTMHREPLFATQALRAGAQ
                     GYVTKSSPPEVLVQAVLQVAAGRPFISPDLAPELALALVDRPRAPLADLAPREFEILR
                     LLLEGLDPEEIGRRLSLSAKTVRNCHYQIKSKLGVRSDIELTRLAMAHGLV"
     gene            complement(5270..5713)
                     /locus_tag="PLH85_05380"
     CDS             complement(5270..5713)
                     /locus_tag="PLH85_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HNB64024.1"
                     /translation="MHTFDERSIPWQPFGDLPHFVFAIQHVDRAHRTADVLFKFAAGQ
                     QIVLHRHKALNHTFVVQGEHRLYEPDGRLREVRPAGTYTVTPASETPHREGGGATDAV
                     VLFSMRPAPGEILYELLDDDGRVIAELTLETLADLFDAARAPAVA"
     gene            5974..6936
                     /locus_tag="PLH85_05385"
     CDS             5974..6936
                     /locus_tag="PLH85_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64025.1"
                     /translation="MNLHSGATLLALTLLAGTAAADNRFCGGAVVQSGEPTDLAHVVG
                     DQTLEFVADQPASLTVCSKGYLLEKCGDHETALKIFDRCIAAGYVGAMIWKALMLQDG
                     AGTPPDLPAAAELMRRAAHSPDSPYATLGKLHYASALHEGKGVPRDEVAARRWFEAAA
                     ADGNPDAQEFLRTGYHVGDRDLAGAGTGVAPPVPAPAGTPSQEGEPPRLLASTGARGD
                     GLRSAASTSLAARRLGPPAASPPPISELPVVPALLREDALATEVPPDVRGQRLARVEA
                     AAPEAPGSVVSRWLPVALLGTVLAGALGQGRGRGALPAVAGSVA"
     gene            6933..7424
                     /locus_tag="PLH85_05390"
     CDS             6933..7424
                     /locus_tag="PLH85_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB64026.1"
                     /translation="MNAALESLLYEASQLFLVPVLLAIAGLFLYAFYAVGAFLWQARQ
                     RHAGDPAGFELMGLLAAQPDLGVADLEAHAFRRLEVARIATRVAPMLGLVATMIPLGP
                     ALRALGDGHMADVSRGLGVAFSAVILALVAASLTFWVVNVRRRWYASDLLAIEARLSG
                     SGR"
     gene            7421..7738
                     /locus_tag="PLH85_05395"
     CDS             7421..7738
                     /locus_tag="PLH85_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HNB64027.1"
                     /translation="MRLRMHLGEAEDDDPVLSLVNLIDVFLVIVAALLLAVAQNPLGP
                     FAEGKVTVIRGAGTPQMEVVIKDGAKIERFRGDGGAASASGGVRAGTAYRLADGSLVY
                     VPE"
     gene            complement(7742..9823)
                     /locus_tag="PLH85_05400"
     CDS             complement(7742..9823)
                     /locus_tag="PLH85_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64028.1"
                     /translation="MNTPRHLPILNLRQVEGHPDRLCGTVRMPDADRDTHLLVLRRGQ
                     HWSAVPAHCPHEGHRLDQSALDADGRLICPAHGLEVDTQGSRNILPVRRAGDEFIIEL
                     PAADALQLTPVADVQRLQAEVEALRQANAALEQQIGAVTATMDAMVDDVSFQSRELAA
                     RSAEQTRLSAFVTNVMETMDSLLLVLDRFGAIRQANAALRHRLGIDPAGLSGQSPDPL
                     LAPETLTTLAADLPSAGAGTLLFRTILKAGSLEMETGMPSRLAGDPVRHFILRATTLY
                     DRSGKLDGVVIVGSDITALRAREQALRESEQLFRDYSALSTDWFWQTDADMRFTTYVG
                     PPRGGHDLGVVVAGKRREDFADPEDLRNAVKWQAYHAEIDARRQFRDFEYRVVHPLIG
                     TTWFSVSGMPLFAPDGAFLGYRGTAKDITGPKAIEKELRAHRDHLSELVAAQTADLVS
                     AKETAERANQLKSEFLANMSHEFRTPLHGILSYARLGLTRLGQVPPDKIAAYFNRIHQ
                     SGSRLAGLVNDLLDLSKLEARRVDFRLQAADVAVVVERVRGDLAALIASRGLSVAVER
                     ATVSTVAMVDTQQLYHAIQNLLSNAIKFSPDGGVLTLRFADALLGEQDALAFQVRDRG
                     VGIPPGEEERIFDKFIQSSATKTGAGGTGLGLAITREIVRGHQGLIEARNHPDGGAVF
                     EIRLPRRAAED"
     gene            complement(9820..10281)
                     /locus_tag="PLH85_05405"
     CDS             complement(9820..10281)
                     /locus_tag="PLH85_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNB64029.1"
                     /translation="MASEQTHIICFGNALHGDDGFGPTVYTHLGETPLPPGVRLFCAD
                     TAGLGALACFEDCTTAIVVDALRGFGPPGSLHSLAAGELEGSDDAASANIHGAGVSSL
                     LRLLPVALPTPPRVRVIGVEIATLSPFQPGLSAAVAARLPEALAHIRRLLA"
     gene            complement(10271..11806)
                     /locus_tag="PLH85_05410"
     CDS             complement(10271..11806)
                     /locus_tag="PLH85_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNB64030.1"
                     /translation="MPLTTLNIDLNRVEGDLEFQVDLDGDTVVDARCVGTLFRGFEQL
                     LIGRAPRDALVITPRVCGICGTAHLYTAALALERLAQIPVSPHATLVRNLCLMAENVQ
                     SDLRQSFLFFTPDLVHRRYADEPLFADVERAFAPLKGRIVRGALGATRKILELVAIFG
                     GQWPHSSYMLPGGITRGATARDLVDCHDIVAQAREWYEQDVIGDTLEHWLALDSADAF
                     FAWLDTAAPAGSALGLLTQFARSIGLQHIGAGAGHFLSAGSWHDPQRWQPPYGEPTGL
                     IDAGLYDGQHGTVTPFDPALINEHVRHAWYRPYPGGRHPYDGETVPDYQPDSDRYTWA
                     KAPRYGDAVVETGPLAELLVGGDRLIRDLLRQEGPNTWLRQFARLRRAGLTLHLMKRH
                     LDDLAGQLGTPHFLPPPDSALADGEGFGLVQAARGTLGHWIRVEQGRISRYQIITPTA
                     WNASPKDSNGRHGHWEQSVIGVRLADPGDPVEIGHIIRAHDPCLVCTVHVVGRGTRHR
                     YGV"
     gene            complement(11822..12763)
                     /locus_tag="PLH85_05415"
     CDS             complement(11822..12763)
                     /locus_tag="PLH85_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HNB64031.1"
                     /translation="MATLIWLQTGACSGDTLSLLNADSPSIERLTADGTLDILWHPSL
                     TAGPPGHLGTLIAAVEAGAQPLDLLCVEGSLITGPYGSGMFDSYRGRPKIRIVEALAA
                     RARVVVAIGTCAAFGGIPAAPPNPTDCTGLQFDRESPGGLLSPEWRSSAGLPVINLAG
                     CPTHPTTITRALAMLARDQPLELDFLNRPRLFFGAMVHQGCTRNEYHEYDVEETQPGG
                     RGCMYFNLGCQGPSTLATCNSELWNRRSSKTRAGVPCFGCTAPGFPREGDLFRTEKIG
                     AVPVTLPLGVSRAHYVAYKNLAQAAAPLRVKNREMEP"
     gene            complement(12905..13318)
                     /locus_tag="PLH85_05420"
     CDS             complement(12905..13318)
                     /locus_tag="PLH85_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64032.1"
                     /translation="MSPDDGLLDALRLRISPEAGLFLGSLRESFGPLWLLYDGQAPDE
                     RERLRWAPALREPPPEGYSPWACCACGTLLVTDADRSPLAQACLDVDLVEQKPGSTTL
                     SCTGLYLSAHLWPLGGTDLALRRALEAEQRSTAGR"
     gene            complement(13340..13711)
                     /locus_tag="PLH85_05425"
     CDS             complement(13340..13711)
                     /locus_tag="PLH85_05425"
                     /inference="COORDINATES: protein motif:HMM:NF017426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HNB64033.1"
                     /translation="MTERIVATEAAVQFIDELRRDFGPVIFLLSGGCCDGSVPNCFKV
                     GEFMPSQTTLLLGTVGGAEFWLGHQEYIYYENSQLTLDVRPGQGGDFSLDCGRGRAFE
                     TTSRPFTDAELATVLPPVRGA"
     gene            complement(13806..15326)
                     /locus_tag="PLH85_05430"
     CDS             complement(13806..15326)
                     /locus_tag="PLH85_05430"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB64034.1"
                     /translation="MLYALPGTAEAKVQYKSRYDNFIGGKWVAPLKGEYFDVITPITG
                     KPYTQVARSQAEDVELALDAAHAAADKWGKTSPAERSNILLKIADRLEANLEKLAYAE
                     TVDNGKPIRETLNADIPLAVDHFRYFAGCLRAQEGTISEINEDTVAYHIHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALGAGNCVVLKPAESTPISILVLAELIADLLPPGTLNIVNG
                     YGREAGMPLATSKRIAKIAFTGSTATGKIIGAAAASNLIPATLELGGKSPNIFFADVM
                     DKDDGFLDKAIEGMVLFAFNQGEVCTCPSRALIQESIYDKFIERVLPRVAAIKQGSPL
                     DTDTMIGAQASQMQIDKITEYLAVGKQEGAQCLIGGERAHLSGDLSEGYYIQPTLFKG
                     HNKMRIFQEEIFGPVLAVTTFKDEAEAVAIANDTVYGLGSGVWSRNGNVAYRMGRAIK
                     AGRVWTNCYHAYPAHAAFGGYKESGIGRETHHMMLDHYQQTKNLLVSYAEQKLGFF"
     gene            15637..16344
                     /gene="rquA"
                     /locus_tag="PLH85_05435"
     CDS             15637..16344
                     /gene="rquA"
                     /locus_tag="PLH85_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HNB64035.1"
                     /translation="MSSQPSADQAPAAVIEPSLPDYLRETYTWAYLDPRTVPWLDRPL
                     VVSAILWGNARRLMDAAVAEFTPGQRVLQAAAVYGDFSVRLARQLGPEGALTVVDVAR
                     IQVANTRRKLEAWPTARVLRADLTEPQGTTFDAVCCFFLLHEVPPHARQRIVGNLLAA
                     VEPGGKIVFVDYHRPRWWHPMGPVMALVFRFLEPYARSLLAESIDTLVPAAADFDWHS
                     ETRFGGLYQKVVGVRRR"
     gene            complement(16355..17155)
                     /locus_tag="PLH85_05440"
     CDS             complement(16355..17155)
                     /locus_tag="PLH85_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNB64036.1"
                     /translation="MPRLPCRIVCLSTETVEVLYLLGEAARIAGISGFTTHPPQARKE
                     KPKVSGFSSAKIDRILAVEPDLVLAFSDLQGEIARDLVRAGVAVHVFNQRSVDGILAM
                     VETVGRLVGAEARALALVAGLETDIAAARAAGEARIARLGRRPRVYFEEWNDPLISGI
                     RWVSELIHLAGGEDCFAELAVHAGAKARILSGPEAVIPRAPEVIVGSWCGKHFQPAQV
                     LARPGWDALPAVRDGHVYEVKSAILLAPGVAAIREGLPALAACLDRLG"
     gene            complement(17179..18219)
                     /locus_tag="PLH85_05445"
     CDS             complement(17179..18219)
                     /locus_tag="PLH85_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64037.1"
                     /translation="MKRTAALLLLAATLARAEGGPLPGEQLLGPGQLAPLPADARVVP
                     GYAGTWRRADSQERVRDRIVELEGRRAKPCTDVRVTDTEQAAPDLAETRPTRIHHALE
                     RWTVRACGATRSYEVWYRFAPGASRVAVAENGGGRFQGELDPPYRRLLELAAAHPRDA
                     GAGADPRRWLNLPLPEGWHYASQSGGLPGQDWRTDFLPVGPRLPEWTDLISVQGLPRR
                     PATGQARLYLEGLQASRQTRCGAAPEPIEALPADDATGRARLRTLLICPQVPGTDYAE
                     LALVQALEGPDILYVVQRAWRLPAADADSLRRDSADVLKTGAYFLSLVRLCPPATRMR
                     DCPAPFPADLRP"
     gene            complement(18216..19100)
                     /locus_tag="PLH85_05450"
     CDS             complement(18216..19100)
                     /locus_tag="PLH85_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNB64038.1"
                     /translation="MRRRFPALLLTALLTVSGAARGAIDLADDQGTPVHLAQPARRIV
                     SLAPHVTELLFAAGAGGKVVGAVQYSDYPEAARSVARVGSYTAVDMEKVAALKPDLVV
                     AWKSGNRDAHLDKLRALGIPVFINEPRRIEDVARSLTQLGRLAGTEAAAEAAAQAFRA
                     RQGALAARYSARPTVRVFYEIWNHPLMTINGEHLISDVIRLCGGENVFARLPVLAPAI
                     DTEAVLAAAPEVIVASGMGEARPEWLDDWRRWTKLNAVARGNLFFIPPDQIQRHTPRI
                     LDGAERLCGQLDEARARR"
     gene            complement(19109..20083)
                     /gene="cobD"
                     /locus_tag="PLH85_05455"
     CDS             complement(19109..20083)
                     /gene="cobD"
                     /locus_tag="PLH85_05455"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNB64039.1"
                     /translation="MLEHGGRLREAARRTGIARADWLDLSTGINPHGYPVPSLAAEAW
                     QRLPEVDDGLEAAAAAYYGSAELLPVAGSQAALQALPACFPPGRVLTLAPTYAEHPHA
                     WRHHAVTALPAAALDDDAVDRTDTLLLVQPNNPDGQRFPRTRLLAWHARLQARGGRLI
                     VDEAFADADPVDSLTPLAGTPGLVVLRSLGKFFGLAGARVGFVFAEAALRDALAERLG
                     PWTVAGPARAVATAALQDRAWQTATRHRLAADCRRLAGLLAAHGLPPAAGTALFQWVP
                     HGDAAQIHEALARQGILLRLFTAPTGLRFGLPGTPAHWDRLAAALARL"
     gene            complement(20076..21029)
                     /gene="cbiB"
                     /locus_tag="PLH85_05460"
     CDS             complement(20076..21029)
                     /gene="cbiB"
                     /locus_tag="PLH85_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNB64040.1"
                     /translation="MPGFTLPVATSPVALPLALLMALGLLLDRLLGEPRHHHPLVAFG
                     RIAGRIESLLRLTGSGRAAGLLAWALAVLPWVALAGSLRPLHPWAGALLDATLLYLAV
                     GGRSLAEHAAAVATPLLDGDLGGARQRLSWMVSRDTTQLDESGVANATVESVLENGND
                     AVFGALFWFVLLGGPGALLYRLANTLDAMWGYRNARYLRFGWAAARIDDVLNYLPARL
                     TALSYALLGHTATALRCWRTQAPAWDSPNAGPVMASGAGALDLRLGGPAVYHGHLETR
                     PALGDGARARAADIDRAVALVQNTTWLWLGVIAAAGFALRA"
     gene            21069..22580
                     /locus_tag="PLH85_05465"
     CDS             21069..22580
                     /locus_tag="PLH85_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNB64041.1"
                     /translation="MRRASTLMVQGTTSDAGKSTLVAGLARLLRRRGVAVAPFKPQNM
                     ALNSAVTADGGEIGRAQALQALAAGVPPHTDMNPVLLKPASDIGAQVIIHGRAVAHLS
                     ARDYHDFKPRAMAAVLASFGRLCAGYDAVLIEGAGSPAEINLRDRDIANMGFAEAADC
                     PVILVADIDRGGVFAHLVGTLDLLSASEQARVKGFVINRFRGDIGLLQSGLDWLEART
                     GRPVLGVLPYLHGLFLDAEDALGEACVDKSATRLRVVAPVYPRISNHTDLDALRLHPQ
                     VDFRWVGPGQAMPPADLIVLPGSKAVQADLAWLRAQGWEPALRRHLRYGGKLVAVCGG
                     FQMLGTRLHDPQGLEGEAGSVAGLGLLDAETVLEAEKQLVNVHGRLALPGAAPVSGYE
                     IHMGVTRGPALAQAAVELEDGRRDGAISDDGQILGTYLHGLFDHPDALVALLAWAGAG
                     DVDRVDLAARREADLERLADALEAHLDLPRLAACLGDGTAAEGVRTSLHVPLA"
     gene            22613..22807
                     /locus_tag="PLH85_05470"
     CDS             22613..22807
                     /locus_tag="PLH85_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64042.1"
                     /translation="MDAEFASLEQKLEQLLALHAAAQAENRSLRERLAAVEGANRELN
                     DRLGIAGSRLEALLARIPEV"
     gene            22811..23107
                     /locus_tag="PLH85_05475"
     CDS             22811..23107
                     /locus_tag="PLH85_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNB64043.1"
                     /translation="MEHLDIKLLGREYRVACKPEERDGLMAAVAYLDEKMRDLGAKTN
                     SAGERLAVMTALNITHEFLQFQRAGGFDIQAVKRRIEHMNSRLDGVLAPQEKLF"
     gene            complement(23464..23919)
                     /locus_tag="PLH85_05480"
     CDS             complement(23464..23919)
                     /locus_tag="PLH85_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB64044.1"
                     /translation="MSDLLRSAPARHPVRLVLTLGVAALATQMAGCGNKAAVDEELAA
                     KLTQPVASVVLTAAPAAAPGSRSGEDIYKAVCSACHASGAAGAPKAGDKAAWGPRLGA
                     GLAGLVKSATNGKNAMPPKGGAADLTDTELTRAVAFLANQAGAGFKEPQ"
     gene            24066..24142
                     /locus_tag="PLH85_05485"
     tRNA            24066..24142
                     /locus_tag="PLH85_05485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24100..24102,aa:Arg,seq:ccg)
     gene            complement(24288..24701)
                     /locus_tag="PLH85_05490"
     CDS             complement(24288..24701)
                     /locus_tag="PLH85_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64045.1"
                     /translation="MPAPAHTDFDIALIEDSTVLRDLLSAMLADIPNIRLVGAAASEA
                     EGLKLLQATRPRLVIVDLELREGTGLRVLAALQRQPDHFGSPQAVVFSNHAHSVVQAR
                     CRMLGARAFFDKSFQTDDLLAFIEDAAGCPATASH"
     gene            complement(24961..25227)
                     /locus_tag="PLH85_05495"
     CDS             complement(24961..25227)
                     /locus_tag="PLH85_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64046.1"
                     /translation="MRGIQNTPQEPKWALPGKWDWSVRLSLVSSVALCAGMLYGSSVG
                     HQGARPRLESTSCLPDRHDVDRLIGPAAWTSVTDCVLLPRDTSH"
     gene            complement(25556..25867)
                     /locus_tag="PLH85_05500"
     CDS             complement(25556..25867)
                     /locus_tag="PLH85_05500"
                     /inference="COORDINATES: protein motif:HMM:NF039524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64047.1"
                     /translation="MSATHSTANSLRQIANDLGDSARDALPPVRKQLGITADTTREAA
                     TSLMGNLRTAARDTVGIVRRDAAHASEAAGAYIRRKPLQSAAVVGVVGLLIGALLGRR
                     F"
     gene            complement(25924..>26239)
                     /locus_tag="PLH85_05505"
     CDS             complement(25924..>26239)
                     /locus_tag="PLH85_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNB64048.1"
                     /translation="ASLLALALASVPATQAATAAQGDRQAPRSAAARFIDDASITANI
                     KARHAEDRSVRITAIGVETRNGVVQLSGFTPSPAEKTRAEALAWTIPGVKAVQNDIIL
                     IN"
     assembly_gap    26240..26339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26750..28672
                     /locus_tag="PLH85_05510"
     CDS             26750..28672
                     /locus_tag="PLH85_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNB64049.1"
                     /translation="MNMPISPRSRRRLRHVAAILAVAGLGVALFDGNLLRGPLARLAS
                     AQLGRSVQIAGPVDLHPWSLHPTLSVGGVTVANVTGGRASLLASAGRLAVTADLRALL
                     RGAVVLDALEVDRAEIHLEEDREGRGNWQLGGQIGAGGKDPAAAPTRLRIARVRIHDS
                     RLSAELPSRKTSLQLEVATGDDGRIRFTARGRYQGQDLTVDGEAGSVPGLIDGQLPYP
                     LKAAGTVGATRFRIDGSSADLAGLNGLDVGFELAGRSLAELYPLTGVPLPASPAYRFA
                     ARLVQSGTLWRFENIDGTVGRSDLAGTFAVDRGPVPQRLSGTLRARHLDLQDLSGFLG
                     ARDSSGERLAPRPGKVLPARPLGFDKIAAADVDLDFAIGDIRHSGLPLDAASGHLRIV
                     DRRVLLQPLRVGMAQGEVSGVLELETRGMPARARLDLQASRLKLRELMPTLESRSLTT
                     GVLGGRATVSMRGNSVAALLGSATGDLALAMDGGSTSRLLVRLANLDVANAFVSWLAG
                     GQREDIRCLVGDFSARDGVLEPRALLLDTERTLIRGEGQINLRDETLDLRLRASARDG
                     SLLSLRGPLHLDGSFADPKVLPEPVPLGSRVAGAVALGLISPPLALLPLLEAGDARES
                     ACKRLLQQAGKTMGRP"
     gene            complement(28789..28956)
                     /locus_tag="PLH85_05515"
     CDS             complement(28789..28956)
                     /locus_tag="PLH85_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNB64050.1"
                     /translation="MLHYAVVFFVIAIVAALFGFGGIAAGAVEIAKVLFFIFLILAIV
                     SFVFNLLLGKG"
     gene            complement(29012..29215)
                     /locus_tag="PLH85_05520"
     CDS             complement(29012..29215)
                     /locus_tag="PLH85_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNB64051.1"
                     /translation="MNHNIIEGNWKQLKGKLKEHWGKLTDDDLDVIEGRREVLAGRIQ
                     ERYGLTLDEAKRHVQAWEKMLDA"
     gene            29597..29779
                     /locus_tag="PLH85_05525"
     CDS             29597..29779
                     /locus_tag="PLH85_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64052.1"
                     /translation="MKRVSIDWNAKPVRAARRSPARSVWTDTLEDEIDLRRLRTERRL
                     DKVRRALINQSGLTSR"
     gene            29850..31244
                     /locus_tag="PLH85_05530"
     CDS             29850..31244
                     /locus_tag="PLH85_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNB64053.1"
                     /translation="MNTVLPLRDPRARMRLVRHWPVFLVILGVGLLLGLLTIGRLHSE
                     FGVSALRTSQAYNSQIDRLNRLLIVLLDAETGVRGFIASGLNPVYLEPYQQAEQDIGP
                     LLKAISNDYPQGTQDHTEFELLGQLIAVKRQLLAETVRIRRVPTVVPPGETGPGKAYM
                     DQIRLSIAVLRGRLEERNARSIAESTKRFNDTRTLVTVLALGAVGLVLALFAVQQRQT
                     VLRARIAELLERENIALESTVAERTRELSDLASYLTTARELEQARLARELHDELGALL
                     TAAKMDASWLLRSLGAQSTPDIQARFRRLIDTIGSGITIKRRVVSDLRPPLLQGLGLV
                     EALRALSDDLHDEYRVTLQLPGENIDCDEEQALALFRIVQESVTNIRKYARARAIEVG
                     LAVDAGVVRLWVKDDGVGFDPDNPQLNRHGLAGMKHRVQMFEGRFTVTSAPGAGTRID
                     ATMPLRPAAPSAHG"
     gene            complement(31307..31942)
                     /locus_tag="PLH85_05535"
     CDS             complement(31307..31942)
                     /locus_tag="PLH85_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB64054.1"
                     /translation="MLRLAIVDDHKIVRAGFREMLSDELGIQVEFEAASGEEALQHLR
                     TTPCDVLLLDLSLPGQSGVDVLRAVRQRYADLKVLVLSGFPEERYALPMIRNGANGYL
                     CKDCERDELIRAIRTVAQGRRYVSPRTAELLADDLAGAGTALPHESLSERELQVFLRL
                     ARGESVSGIADVLNLSVKTVSTYRSRLLEKLDVASNAELAAYALRHGLIVD"
     gene            32181..32591
                     /locus_tag="PLH85_05540"
     CDS             32181..32591
                     /locus_tag="PLH85_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNB64055.1"
                     /translation="MKIVRRRPLALEASVRIGLLAVTFVAAFAAGHSTASRAPQSLDD
                     PAAGPALLRDGVDRAGRSVAAAVGLDVYSEAGRPVGRLTALLHGEGHDGLARIAVAER
                     GDEGAREIVVPLGRLVWRDDRVVLADGAAPRWLI"
     gene            32602..33795
                     /locus_tag="PLH85_05545"
     CDS             32602..33795
                     /locus_tag="PLH85_05545"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNB64056.1"
                     /translation="MATAAARPQDRATRLKVRRARKMARSAHAYVRGSTARFYAWLAA
                     SDGDALPDGPPVWICGDCHLGNLGPVADSDGRTGVEIRDLDQAVIGNPAHDLVRLGLS
                     LACACRSADLPGVMSLRVVDMLLDGYARAFAQRPRPPSPPDSVKAVIRRAVKRTWKQL
                     MRDHLDHARPRIPLGARFWPLTRREQGAIAQCLDETRLPELARQLGIARDTEAVRVAD
                     AAYWVKGCSSLGRPRYVVLLQVDGAAEARLAMVDIKDAPPAAAPRTGSVSMPRDNAER
                     VVEAARALAPALGGRMVAMRLLDRPMVARELSPQDLKIEPDQLAPADMAALAHYLGRI
                     VGDAHARQMSAPQRRHWHATLRRSHSRVVDAPSWLWRAIVDALADHERGYLEHCRRHV
                     AALVA"
     gene            33973..34368
                     /locus_tag="PLH85_05550"
     CDS             33973..34368
                     /locus_tag="PLH85_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64057.1"
                     /translation="MPSEPTVLRTVIIEDNAVLLDLLTGMLAGIEGVSVVGHAASEAE
                     ALALVRATRPQLAIVDLELRGSSGLGVLAAFQQTPDEYGAPLAIVFSNHAHPIVQSRC
                     LALGARAFFDKSFQMDDLLDFVQATARAL"
     gene            complement(34464..34775)
                     /locus_tag="PLH85_05555"
     CDS             complement(34464..34775)
                     /locus_tag="PLH85_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNB64058.1"
                     /translation="MKTTAVTLALAGLLAFSAAGCAITREQSTVGQYVDDATITTRVK
                     AKFAEDSTVSAMAIGVETLKGTVQLSGFAKSTTERDRAESLARGTPGVSMVKNDIVVR
                     P"
     gene            35166..36671
                     /locus_tag="PLH85_05560"
     CDS             35166..36671
                     /locus_tag="PLH85_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene/methane monooxygenase
                     hydroxylase/oxygenase subunit alpha"
                     /protein_id="HNB64059.1"
                     /translation="MALLNRMDWYDLARSTNWTPKYVTEDELFPPKLSGEFGLPLTAW
                     ESYDEPYKQTYPEYVKVQREKDAGAYSVKAALERSRIFENADPGWKSVMKAHYGAIAR
                     GEYAAASAEARMMRFSKAPGMRNMSTLGCMDEIRHGQMQLYFPHEHVAKDRQMDWAFK
                     AYDTNDWAMIAARHFFDDIMMTRDAISVSIMLTFSFETGFTNMQFLGLAADAAEAGDH
                     TFANLISSIQTDESRHAQIGGPALKVLIENGQKAEAQKRVDIAVWGAWKLFSVLTGPI
                     MDYYTPLEHRKQSFKEFMEEWIVAQFERALTDMGLELPWYWEIFLKDLSETHHGMHLG
                     SYYWRPTVWWNPAAGVTPDERQWLEEKYPGWNDTWGQCWDVIIDNVVDGNMAKAYPET
                     LPYVCNMCQLPILGTPGKGWNVKDYPLEYKGRLYHFGSEVDRWVFQQEPERYAGHLSI
                     VDRFLAGMIQPMNLEGALAYMNIAPGECGDDAHNYAWAEVYRALRAARKAG"
     gene            36728..36988
                     /locus_tag="PLH85_05565"
     CDS             36728..36988
                     /locus_tag="PLH85_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene-4-monooxygenase system B family protein"
                     /protein_id="HNB64060.1"
                     /translation="MALFPLTSNFEGDFVLQLLPVDTEHTMDEVAAAAAVHSVGRRVR
                     ARPGHILRVRRHGSAEFLPRTLKVGEAGFQPTETVDIIWQAA"
     gene            37014..37349
                     /locus_tag="PLH85_05570"
     CDS             37014..37349
                     /locus_tag="PLH85_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNB64061.1"
                     /translation="MCFTKVCTLDDVWEGEMAAFTVNGHEILLVCPEGGEIKAFQGVC
                     PHQDIALAEGKFDGKRVICRAHLWQFDAATGQGVNPDDCALAVYPVKVEGDDVLVSTE
                     GVEPLFAHT"
     gene            37408..37722
                     /locus_tag="PLH85_05575"
     CDS             37408..37722
                     /locus_tag="PLH85_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HNB64062.1"
                     /translation="MSTAAAGQAYHNNLVGPVMRAGDLAMAVIEAAKVDNPGKDIYVD
                     DKRAYIRIHAEQEMILRQATIEEELGRPFRMNDLEVDLSSFAGQIESRDDSVRFYFVK
                     KL"
     gene            37766..38752
                     /locus_tag="PLH85_05580"
     CDS             37766..38752
                     /locus_tag="PLH85_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HNB64063.1"
                     /translation="MSEIQVLKPQKTWSHLAVRRRKPSEYEIVSANLHYNDRDPNAPY
                     ELDPGMFMNEWYKKHTFGTALKHADWNAFRDPDEVVYRTYNMMQDGQETYVFGLFDQF
                     NEREHDKALDPRWAGTLARLYTPARYLFHTLQMASAYVGQVSPASTITNCHYFQMADS
                     LRWLSHTAYRTTELARAFADKGFGRDERHYWEEDAVWQGFRELMEKALTVWDWGEAIV
                     VLNLIVKPAVEETVLRRLGESARHNGDTLLGLLTDAQLIDVARHRRWAAAFVAMALET
                     PGNRELIAGWIAQWEPLADRAIDAYCAALPDVPEAAAAARAATRDLRRSLGF"
     gene            38815..>39239
                     /locus_tag="PLH85_05585"
     CDS             38815..>39239
                     /locus_tag="PLH85_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNB64064.1"
                     /translation="MNPPLIVNERDGSRFAQAPGDTVLRAALRAGIGLSYECNSGGCG
                     GCKIELLQGDVETLWPDAPGLGERDRRRGRHLACQCRAVGDITIKAPTAPEYVPPILP
                     RRRRAFLIGTTDLTHDLREFRFAADGPADFLPGQYAMLDL"
BASE COUNT         5664 a        12915 c        14552 g         6008 t
ORIGIN      
        1 gccctgttca ccagcctgat ggcggtgccg cgcaccttca tcaacgcctc cgccggctgg
       61 ctggtggagg atctgggctg gttcaggttc ttcctcctgt gcggcttgct cgcagtgccc
      121 ggcatgctgc tgctgtttag ggtcgccccg tggcgcctgg cggatgattc cagtgaaggg
      181 atgaaatgat gggaaaagga cgcctggagg ccttcagtga cggcgtgttg gcgatcatca
      241 tcacgatcat ggtgcttgag ctcaaggtcc cccatggcga tgacctggcc gcgctggccc
      301 cgctgctgcc agtgtttctc agctacctgc tgagcttcgt ctatgtcggc atctactgga
      361 acaaccacca tcacctcctg catgcagccc acggggtgac gggggcggtg ctgtgggcca
      421 acctgcatct gttgttctgg ctgtcgttga ttccctttgt cacggggtgg atgggcgaga
      481 atcacttttc cgccttgccg acggcgctct atggcggcgt gctgttgatg gcggcgatcg
      541 catacttcat cctgcagtgg gcgatcctgc ggggcgatgg cactgcctcg gtgctggccc
      601 aggcgctggg ccgcgactgg aagggcaaac tgtcgccggt gatctacatc gtcgcgattc
      661 ctgcagcctt ctttgccgaa tgggtctccg acgcgctgta cgtgctggtg gcactgatct
      721 ggctggtgcc ggaccggcgc atcgagcggg cccttgtcgt cgacgagcac ccctgaatgc
      781 ctgctttgat ggatctcccc acgccatgac tgccctcgaa gccgccgccc gcgaactctc
      841 cgccgccctc gcgccgatgc gcttcgcggc gcccgtcagt cacgtttaca accctttggc
      901 gtacgcctgg gcgccctacg cggactacct gcggcgcttc ggccaggggc gcaagcgggt
      961 ggtgctggtg ggcatgaacc ccggcccctt cggcatggtg cagaccggcg tgcccttcgg
     1021 cgaggtggcc gcggtgcggg actggatggg catccgtgcc gaggtgacgg cgccggccgc
     1081 gcagaacccc aagcggcccg tggagggctt cgcctgcacc cgctcggagg tgagcgggcg
     1141 gcgcctgtgg gggctgttcg ccgagcgctt cggcccggcg gaggccttct tcgccgagca
     1201 cttcgtggcc aactactgcc cgctggcctt cttcgatggc gggcgcaacc tcacgccgga
     1261 caagctgccc gccgccgagc agggaccgct gctggcagcc tgcgacgcgc atctgcggcg
     1321 ggtggcggag atcctccagc cggaatgggt gatcggcgtg ggcgcgtggg ccgagctgcg
     1381 cgcccgcgag gcgctggcgg gtctgcccct gcgcttcggg cgggtgctcc atcccagccc
     1441 ggccagcccg gcggcgaatc ggggctgggc cgaggcggcg acgcgccagc tggtggacct
     1501 gggcgtgtgg ccggcctgag gggcgctcag ctggagcagc tcaccgccag atgctgggcc
     1561 cagtccggcg gcggggcggc gtagcgttcg aattcgggct gctcgtcgaa gggccgcgac
     1621 aggcaggcta gcacctcggc ggtgatgcgg tcatcgccct gctgggcggc gcggatggcc
     1681 tcctcggcga cccagttgcg cagcacgtac ttgggattga cggtgagcat cgccgcctgg
     1741 cggacgtcgt cgcggctgcc ttcggcggcg aggcgcgcgc gccacagggt gagccaggcg
     1801 tcgcaggcgt cccggtcgat gaagtgatcg cgcaggggcg cgtccacggt ggcgcggctg
     1861 gcgtcggcga ccgtgccggg caggcgcgac aggacgcgga agaaggtggt gtagtccggg
     1921 tggaagcgtt gcagcaggga cagggtctcg ccgatgaagt cctcgtcacc gggcagggcg
     1981 tcctgcaggc cgagcttggc gcgcaggccg tttaggtagc cgttctcgaa cgccggcacg
     2041 tagctgtcgg cgatggcggc cttcacgtcg tcctggctgc ccaccaggct gcgcagggcg
     2101 cggccgaggg cgaagaggtt ccagtggccg atctggggct gggccttgta ggcgtagcgg
     2161 ccgtggtcgt ccgagtggtt gcagatgtgg ttggcgtcga agccgtccat gaagccgaag
     2221 gggccgtaat ccagggtgag gcccagaatg gacatgttgt cggtgttcat caccccgtgc
     2281 atgaagccca ccgtctgcca acgggcgatc aggtcgccgg tgcggcggcc ggcgtcccgc
     2341 agcagggcgt cgtaggggtt ggcggcgtcg cgcagttcgg gacggaagcg atcgatgacg
     2401 tagtcggcga ggacgcggag ggcgtccggc tgcttgttca ccgcccagtg ctcgaaggag
     2461 ccgaagcgca ggaagctggg cgccaggcgg gtgcacaccg cggcggtctc gacggtctcg
     2521 cggcgcacgg gcaggggcga gcccaccacg cacagggcgc gggtggtggg gatgccgagg
     2581 gccgccatgg cttcggagca gaggaattcc cgcaggctgg agcgcagcac cgcgcggccg
     2641 tcggcaaagc gcgagtaggg cgtctggccg gcgcccttga gctggatctc ccagccggcg
     2701 ccggcgcccg gcacctcgcc cagcagatgg gcgcggccgt cgccgagctg gccggcccac
     2761 atgccgaact ggtggccgga atacaccgcg gccaagggct cgctgccggg cagcagggcg
     2821 ttgccggcaa atacccgggc gaactccggg cgggcgaatt cggccgggtc caggtcgatc
     2881 agcgcggcgg cggcggggct ggccgccacc agcacgggct cgggcagcgg gtccggcgca
     2941 aggcgggtgt agaactgctc gggcaggcgc gcgaacgcgt tgtcgaaggg caggttttca
     3001 agggtgcgca tggagggctc cggtggcggc gcatcgggca aattgcccac gcgctccgcg
     3061 gtaaagtgcc ggcaggcggg acggcggtcc ggcctaaaat ccgactattt cactatggta
     3121 tggttccgcg gcccccacgg gaagcgggcc gacgcaaaaa tgctgcaaac gcacatcgac
     3181 gcgcccgcgg atgccgggcg gcccgccgcc ccgttgcccg attccggcgt ggatctgaag
     3241 cggtatctcc tgtggcgggt gagcctgttc gccctggcca tcggcctcgc ggcggtggcg
     3301 gcggtcttcg tgcagaccgg cgagcgcctg cgcggccaca tcgcccgcac cggcagcacg
     3361 gtcgagaatc tcctcgccgc cgaggtggat cgtccgcggg acagcttccg gcgcaccgtg
     3421 gagggcctcg acctcacctc gctggacggc atcggcccgc tgctgggcat ctgcgtgaag
     3481 gtggaggaca tctacaagcg ggcggtgatc cggcgctgtt tcgacgaggg cgagggcgcg
     3541 cctgccccag tgcgggccgt gctggccgcc ctgctggggc ccgacgtcgc ctaccggggg
     3601 gtgatcggcc agtatcccgg cgtgaaggtg ggcgaattca ccgtgacgcc ggacctggac
     3661 gccgccgcgc gggacgcctg gagccgcatc ggcatggtgc tcgggatgac tttcggcatc
     3721 ctcctcctca acgggctggt ggtgttgccc gtgcgccgcg ccctggcgcc ggccgagcag
     3781 atcctggcgg tgctggcgcg catggaggcg ggcgacctgg ccgcgcgcat gccgcgcccg
     3841 cggctgatcg agctgcgccg catcgcggcg ggcttcgacc acctggtgga acgcctggaa
     3901 gccaccctcg ccgcccgccg ccagcttgcc cagcgtctgc tggcggtgcg ggaggaggag
     3961 cgccggcacc tggcgcggga gctgcacgac gaatttggcc agtgtctcgc ctcggtgagc
     4021 gccgaggcgg ccttcatcgg cgcccgcgcc gagcgcagcg atccgtccct gctgcccgcc
     4081 gcccgcgccg tgtcggcggt gaccgcccac atgatggaga gcctgcaggg catcctgcac
     4141 cagttgcgac cagtgggtct ggaggagttc ggcctcgccg ccgctctgga gcagctggtg
     4201 ggcggctggc ggcggcgtca gccggactgt cagtttcatc tggagatgcg cggtgcggtg
     4261 gacgatctgc ctgacgagct gaccgtgagc ctctaccgca tcgtgcagga aagcctcacc
     4321 aacgccctgc gccacggcgc gccgcggcgc gtggcggtca gtctggtgcg ggatgcggac
     4381 ggctgccggg tgaccgtgga caacgatggc cagggccgcg ccgaaccgaa cccgggcagc
     4441 ggcctgggcg tgctgggcat gcgcgaacgg gtcgaggccc tgggcgggca gttcgacctg
     4501 ctgccgctta cgccccaggg catgcgcgtg gcggcagtgc tgccggtcgc ggctctgatc
     4561 cctgtggaga catcccatgc ttgaaccgac ccgcctgctg ctggtggacg accacgcggt
     4621 ggtccgcgaa ggctatcgcc ggctgctgga aacccgcgcc gagcttcgta tcgtcggcga
     4681 ggcggccacc gcccgcgacg ccctcgacgc cttccgccgc ctggcgccgg acgtgctggt
     4741 gctggacctg ggcctgcccg acatgggcgg cgtggagctg atccggcgcc tgctccagcg
     4801 ggaacccggc gcgcgcatcc tggtgttcac gatgcatcgc gaaccactct tcgccaccca
     4861 ggccctgcgc gccggcgcgc agggctatgt gaccaagagc agtccgccgg aggtgctggt
     4921 gcaggccgtg cttcaggtgg cggccgggcg gcccttcatc agccctgacc tggcgccgga
     4981 gctggccctg gccctggtgg accggccgcg ggcgcccctc gccgacctgg cgccgcggga
     5041 gttcgagatc ctgcgcctgc tgctggaggg gctggacccg gaggagatcg gccggcgcct
     5101 gtccctgtcg gccaagacgg tgcgcaactg ccactatcag atcaagagca agttgggcgt
     5161 gcgcagcgac atcgagctga cccgcctggc gatggcccac gggctggtgt gagggcgccc
     5221 ggcccaccgc cgcgggcgtg ggcacgctcc ggcgggggcg ccggcggctt caggcgacgg
     5281 cgggtgcgcg ggcggcgtca aagagatcgg cgagggtctc cagggtgagc tcggcaatca
     5341 cgcggccgtc gtcgtcgagg agttcgtaga ggatttcgcc gggtgccggg cgcatgctga
     5401 agagtacgac ggcgtccgtc gcgccgccgc cttcccggtg gggcgtctcg ctggcgggcg
     5461 tgacggtgta ggtgccggcg gggcggactt cccgcaggcg gccgtcgggc tcgtagaggc
     5521 ggtgctcgcc ctgcacgacg aaggtgtggt tgagggcctt gtggcggtgc aggacgatct
     5581 gctggccggc ggcgaacttg aacagcacgt cggccgtgcg gtgcgcccga tccacgtgct
     5641 ggatggcgaa cacgaagtgg ggcagatcac cgaagggctg ccaggggatg gaacgttcgt
     5701 caaaagtgtg catggaggtc tccggaagga cggggcggtg aatgagcccg ggcggacgcc
     5761 gcccggtgcc ggcattggac gcccggatcg gcctcaccgc cgagggccgc gtgcccgcgg
     5821 gacaacgggc atccggcccg cccgggggca gatgcgatgg cgggcaaaat gcccgcccgc
     5881 gcggggcgaa atgccgctga cgccggtccg gtcgatgcct acagtggggg gcgaagcggg
     5941 ccgccggcct gcattgcctg gacggggaca gccatgaacc tccactccgg cgccacgttg
     6001 ctggcgctga ccctgctggc cggcacggcc gcggcggaca atcgcttctg cggcggcgcc
     6061 gtggtccaga gcggcgagcc gaccgacctg gcccacgtgg tgggcgacca gacgctggaa
     6121 ttcgtggccg accagccggc cagcctgacg gtgtgcagca agggctacct gctggaaaag
     6181 tgcggcgatc acgagacggc gctgaagatc ttcgaccgct gcatcgccgc cggctacgtg
     6241 ggggcgatga tctggaaggc gctgatgctc caggacgggg ccggcacgcc gccggatctg
     6301 cccgccgccg ccgagctgat gcgccgggcg gcccacagcc cggattcgcc ctacgcgacc
     6361 ctgggcaagc tgcactacgc cagcgccctg catgagggca agggcgtgcc gcgggacgag
     6421 gtggcggcac ggcgctggtt cgaggccgcg gcggcagatg gcaacccgga tgctcaggag
     6481 ttcctgcgca ccggctacca cgtgggcgac cgggatctgg ccggcgccgg caccggcgtg
     6541 gcgccgcccg ttccggcacc cgcggggacc ccgtcgcagg agggcgaacc gccgcgcctg
     6601 ctcgcgtcga ccggggcgcg tggcgacggc ctccgatcgg cggcgtccac gtccttggcg
     6661 gcgcgccgtc tcggaccacc cgccgcgtcg ccgccgccca taagcgaact gccggtggtg
     6721 ccggcgctgc tgcgcgaaga cgccctggcg accgaggtgc cgccggatgt ccgcggccag
     6781 cgtctggcgc gggtggaggc tgccgcaccg gaggcgcccg ggagtgtcgt gtcgcgctgg
     6841 ctgccggtgg ccctgctggg gacggtgctc gcaggggcgc tggggcaggg gcgcggacgc
     6901 ggagcgctgc ccgccgtggc cgggagcgtc gcatgaacgc ggcgctggaa tccctgctct
     6961 acgaggcgag ccagctcttc ctggtgccgg tgctgctggc catcgccgga ctgttcctgt
     7021 atgccttcta cgcggtcggc gcctttctgt ggcaggcccg ccagcgtcac gccggcgatc
     7081 ccgccgggtt cgagctcatg ggcctgctgg cggcgcagcc ggatctgggc gtggcggacc
     7141 tggaagccca tgccttccgc cgcctggagg tggcgcgcat cgccacccgg gtggcgccga
     7201 tgctgggtct ggtggcgacc atgatccccc tggggccggc cttgcgggcc ctcggcgacg
     7261 ggcatatggc ggacgtgtcg cgggggctcg gagtggcctt ctcggcggtg atcctggcgc
     7321 tggtggcggc gtccctgacc ttctgggtgg tgaacgtgcg gcggcgctgg tacgcgagcg
     7381 acctgctggc cattgaggcc cgcctctcag ggagcggacg atgaggctgc gcatgcatct
     7441 gggcgaggcg gaggacgacg atccggtgct gtcgctggtg aacctgatcg acgtgttcct
     7501 ggtgatcgtg gcggccttgc tgctggcggt ggcgcagaac cccctggggc cctttgcgga
     7561 gggcaaggtg accgtgatcc gcggcgccgg cacgccgcag atggaggtgg tgatcaagga
     7621 cggggcgaag atcgagcgct tccgcggcga tggcggtgcc gcctcggcaa gcggcggggt
     7681 gcgggcgggg acggcctacc ggctggcgga cgggtccctg gtctatgtgc cagagtgagg
     7741 gtcagtcctc cgctgcccga cgggggaggc ggatttcgaa cacggcgccg ccgtcggggt
     7801 gattgcgggc ttcgatgagg ccctggtgtc cgcgcacgat ctcgcgggtg atggccaggc
     7861 ccagtcccgt gccgcctgcg ccggtcttgg tggcgctgct ctggatgaac ttgtcgaaga
     7921 tgcgctcctc ctcgcccggc ggaatcccga cgccccggtc ccgcacctgg aaggccaggg
     7981 cgtcctgttc gccgaggagg gcgtcggcaa aacgcagggt caggacgccg ccatcgggcg
     8041 agaacttgat ggcattggac agcaggttct ggatggcgtg gtagagctgc tgggtatcca
     8101 ccatggcgac ggtgctgacg gtggcgcgct cgacggcgac gcttagcccc cggctggcga
     8161 tgagggcggc gaggtcgccc cggacgcgct cgacgaccac cgccacgtcg gcggcctgca
     8221 ggcggaagtc cacgcggcgg gcttccagct tggagaggtc gagcaggtca ttcaccagcc
     8281 cggcgaggcg gctgccgctc tggtggatgc gattgaagta cgcggcgatc ttgtcgggcg
     8341 gcacctgccc caatcgcgtc agtccgagcc gggcgtagga caggatgccg tgcaggggcg
     8401 tgcggaactc atgggacatg ttggcgagaa actcgctctt gagctgattg gcgcgctcgg
     8461 cggtctcttt ggcgctgacc aggtcggcgg tctgggcggc aaccagctcg gatagatgat
     8521 cccggtgggc ccgcagttcc ttttcgatgg ccttcgggcc ggtgatgtcc ttggcggttc
     8581 cccggtaacc gaggaaggcg ccgtccgggg cgaacagcgg catgcccgag acgctgaacc
     8641 aggttgtacc gatcagcgga tggacgacac ggtattcgaa gtcgcggaac tgccggcggg
     8701 cgtcgatctc ggcgtggtag gcctgccact tgacggcgtt gcgcagatcc tccggatccg
     8761 caaagtcctc gcgtcgcttg cccgcgacca cgacgcccag atcgtgcccg ccccgcggag
     8821 ggccgacgta ggtggtgaag cgcatgtctg cgtcggtttg ccagaaccag tcggtggaca
     8881 gggccgaata atcgcggaac agctgttcgc tctcccgcag tgcctgctcg cgggcgcgca
     8941 gggcggtgat gtcggagccg acgatgacca cgccatccag tttgccgctg cggtcgtaga
     9001 gcgtggtggc gcgcaggatg aagtggcgca cggggtcgcc cgccagacgg ctgggcatgc
     9061 ccgtttccat ttcgaggctg ccggccttca ggatggtccg gaacagcagc gtgccggcac
     9121 ctgcactggg gagatcggcc gccagggtcg tgagcgtctc cggggcgagc agcgggtcgg
     9181 gactctggcc gctcagcccg gccgggtcga tgccgaggcg gtggcgcagg gcggcgttgg
     9241 cctgacggat ggcgccaaag cggtcgagca ccagcagcag gctgtccatg gtctccatca
     9301 cattggtgac gaaggcgctc aggcgcgtct gctcggcgct gcgtgcggcc agctcgcggg
     9361 actggaagct cacgtcatcc accatcgcgt ccatcgtcgc cgtcaccgca ccgatctgct
     9421 gttccagggc ggcgttggcc tggcgcaggg cctccacttc ggcctggagg cgctggacgt
     9481 cggccaccgg ggtgagctgc agggcgtcgg cagcgggcaa ctcgatgatg aactcatccc
     9541 cggcgcgccg gacgggcagg atgttccggc tgccctgggt gtccacctcg agcccgtggg
     9601 ccgggcagat caggcgaccg tcggcatcca gcgcgctctg gtcgaggcgg tggccttcgt
     9661 gcgggcagtg cgccgggacg gcgctccagt gctggccacg gcgcagcacg aggaggtgcg
     9721 tgtcgcggtc ggcgtcaggc atccgcacgg taccgcacag gcggtccggg tggccctcga
     9781 cctggcgcag gttgaggatc ggcaggtggc gcggggtgtt cacgcgagca ggcgcctgat
     9841 gtgggccagc gcctcgggca agcgggctgc aacggcagcg gagaggccgg gctggaaggg
     9901 gctgagcgtg gcgatctcga cgccaatgac gcggaccctg gggggcgtgg ggagcgccac
     9961 cgggagcagg cgcagcaggc tactcactcc ggccccatgg atgttggcgg aggccgcgtc
    10021 gtcactgccc tccagttctc ccgccgccag gctgtgcagg gagccgggcg ggccgaagcc
    10081 gcgcagggcg tccacgacga tggccgtcgt gcagtcctcg aagcaggcca gcgcgcccag
    10141 gccggcggtg tctgcgcaga acagccggac cccgggcggc aagggggtct cacccaggtg
    10201 cgtgtagacg gtcgggccga agccgtcgtc gccgtgcagc gcgttgccga agcagatgat
    10261 gtgggtctgt tcagacgcca tagcgatgac gggtgccccg gccgacgaca tgcacggtgc
    10321 acaccagaca ggggtcatgg gcgcggatga tgtgtccgat ctcgaccgga tcgcccggat
    10381 cggccagccg cacgccgatc acgctttgct cccaatgccc gtggcgcccg ttgctgtcct
    10441 tgggggacgc gttccaggcg gtgggtgtga tgatctggta gcggctgatg cggccctgct
    10501 ccacgcggat ccagtgaccg agggtgccgc gcgcggcctg gacgaggccg aagccttcgc
    10561 cgtccgccag ggcgctgtcc ggcggaggca ggaaatgggg cgtgccgagc tgaccggcca
    10621 ggtcgtccag atggcgcttc atcaggtgca gggtgaggcc ggcccggcgc aggcgggcga
    10681 actggcgcag ccaggtgttg gggccttcct gccggagcag gtcacggatc aggcggtcgc
    10741 cgcccaccag cagttcggcg agcgggccgg tctcgacgac cgcgtcgccg tagcggggcg
    10801 ccttggccca ggtgtagcgg tcggagtcgg gctggtagtc gggcacggtc tcgccgtcgt
    10861 acggatggcg tccgccggga taggggcggt accaggcgtg gcggacgtgt tcgttgatca
    10921 gggccggatc gaagggcgtc acggtgccat gctgaccgtc atagagaccg gcatcaatga
    10981 gacctgtggg ttcgccgtag ggcggctgcc agcgttgcgg atcgtgccac gagccggcgc
    11041 tcaggaaatg cccggcgccc gccccgatgt gttgcaggcc gatgctgcgg gcaaattggg
    11101 tgagcaggcc gagcgcgctg cctgcggggg ccgcggtgtc cagccaggcg aagaaggcgt
    11161 cggccgagtc gagggcgagc cagtgctcca gggtgtcgcc gatgacgtcc tgttcgtacc
    11221 attcgcgggc ctgggccacg atgtcgtggc aatccaccag gtcacgggcg gtggcgccgc
    11281 gggtgatgcc gcccgggagc atgtaggagg agtggggcca ctgaccgccg aagatcgcca
    11341 ccagttcgag gatcttgcgg gtggcgccga gggcgccccg cacaatcctg cccttcaggg
    11401 gggcgaaggc ccgttcgacg tcggcgaaca ggggttcgtc ggcgtagcgg cggtggacca
    11461 gatcgggggt gaagaacagg aagctctggc gcaggtcgct ctgcacgttt tccgccatca
    11521 ggcagaggtt gcggaccagg gtggcgtgag ggctgaccgg gatctgggcg aggcgctcca
    11581 gggccagggc cgcggtgtac aggtgggcgg tgccgcagat gccgcacacg cgcggggtga
    11641 tcaccagggc gtcccgcggc gcacggccga tcaggagctg ctcgaatccg cggaacaggg
    11701 tgccgacgca gcgggcgtcc acgaccgtgt cgccgtccag gtctacctgg aactccaggt
    11761 cgccttcgac gcggttcaga tcgatgttga gggttgtcag gggcatggcc ggggtggcgc
    11821 ctcagggttc catttcgcgg ttcttgacgc ggagcggggc ggctgcctgg gcgaggttct
    11881 tgtacgccac gtagtgggcg cgggagacgc cgaggggcag ggtgaccggg acggcgccga
    11941 tcttctcggt gcggaacagg tcgccctccc gcgggaaccc cggcgcggtg cagccaaaac
    12001 acgggacccc cgcgcgtgtc ttgctgctgc gccggttcca cagttcgctg ttgcaggtgg
    12061 cgagggtgct ggggccctgg cagccgagat tgaagtacat gcagccgcgt ccgcccggct
    12121 gggtctcttc aacgtcgtat tcgtggtact cgttgcgtgt gcagccctga tgcaccattg
    12181 cgccgaagaa cagacgcggc cggttgagga aatccagttc gaggggctgg tcccgggcca
    12241 gcatcgccag ggcgcgggtg atcgtcgtcg gatgggtcgg gcagcctgcc aggttgatga
    12301 cgggcaggcc ggcagaggag cgccattccg gggacagcag accgcccggc gattcccggt
    12361 cgaactgcag gccggtgcag tcggtggggt tgggcggtgc ggcaggaatg ccgccgaagg
    12421 cggcgcaggt gccgatggcg acgaccaccc gggcgcgggc ggcaagcgct tcgacgatcc
    12481 tgatcttggg gcgtccgcga tagctgtcga acatgccgga gccgtagggg ccggtgatca
    12541 ggctcccttc cacgcagagc aggtccaggg gctgggcacc tgcctcgacc gccgcgatga
    12601 gcgtgcccag atggcccggc ggccctgccg tgagcgacgg gtgccagagg atgtcgagcg
    12661 tgccgtcggc ggtgaggcgt tcgatggagg ggctgtcggc gttcagcagg gacagggtgt
    12721 caccgctgca ggcaccggtc tgcagccaga tcagggtggc catggggacg atctccagag
    12781 tccgtcatgc ctcctctcgg aaggcgaacg gggcgcgcac cggtacgcga caggatgtac
    12841 cggaagcttg ccgcctctgg gtctacgtcg tgtgccgtga gaacttgagt ccgctgctca
    12901 ggattcagcg gccggcagtg gaccgttgtt cggcctcaag ggcccggcgc agggccagat
    12961 cggtgccgcc aaggggccac aggtgcgccg acagatacag cccggtgcag ctgagcgtgg
    13021 tgctgccggg cttctgctcg accagatcca cgtcgaggca cgcctgggcc aggggcgacc
    13081 ggtccgcgtc ggtcaccagc agggtgccgc aggcgcaaca ggcccagggg ctgtagccct
    13141 caggcggggg ctcacgcagg gcgggcgccc agcgcaggcg ttcccgttcg tccggtgcct
    13201 ggccgtcgta gagcagccac aagggaccga agctttcacg cagggagccc aggaacagcc
    13261 cggcttccgg gctgatgcgg agcctcaggg cgtcgagcag gccgtcgtcg ggggacattg
    13321 cggtggccgg gccaaggctt cacgcgccgc gcacgggcgg cagaacggtg gccagttcgg
    13381 cgtcggtaaa ggggcgcgag gtggtctcga aggcgcgtcc gcggccgcag tcgagggaga
    13441 agtcgccgcc ctgaccgggt cgcacgtcga gggtgagctg gctgttctcg taatagatgt
    13501 attcctggtg gccgagccag aactcggcgc cgccgaccgt gcccagcagc agggtggtct
    13561 ggctgggcat gaattcgccc accttgaagc agttggggac gctgccatcg cagcagccgc
    13621 cggacagcag gaagatcacc gggccgaagt cgcggcgcag ttcgtcgatg aactggacgg
    13681 cggcctcggt ggcgacgatg cgttcggtca tggacggatc tccggaatcg aacaaaagca
    13741 ctggacgaaa aaaacggggc gagagactcg ccccgtaaca ccttgcctta catgaaacgc
    13801 tcagatcaga agaagcccag cttctgctcg gcgtagctga ccagcaggtt cttggtctgc
    13861 tggtagtggt cgagcatcat gtggtgggtt tcgcgaccga taccggactc cttgtagcca
    13921 ccgaaggcgg cgtgggcggg gtaggcgtgg tagcagttgg tccacacgcg gccggccttg
    13981 atggcgcgac ccatgcggta ggcgacgttg ccgttgcggc tccacacgcc ggagccgagg
    14041 ccatagacgg tgtcgttggc gatcgcgacg gcttcggctt cgtccttgaa ggtggtcacg
    14101 gccagcaccg gcccgaagat ctcttcctgg aagatgcgca tcttgttgtg gcccttgaac
    14161 agggtcggct ggatgtagta gccctcggag aggtcgccgc tcaggtgggc gcgctcacca
    14221 ccgatcaggc actgggcgcc ttcctgcttg cccacggcca ggtattcggt gatcttgtcg
    14281 atctgcatct gggacgcctg ggcaccgatc atggtgtcgg tgtcgagggg gctgccctgc
    14341 ttgatggcgg ccacacgggg cagcacgcgc tcgatgaact tgtcgtagat ggattcctgg
    14401 atcagggcgc gggacgggca ggtgcacact tcgccctggt tgaaggcgaa cagcaccatg
    14461 ccttcgatgg ccttgtcgag gaagccgtcg tccttgtcca tcacgtcggc gaagaagatg
    14521 ttgggggact tgccgcccag ttccagcgtg gcggggatca ggttggaggc tgccgctgca
    14581 ccgatgatct tgccggtggc ggtggagccg gtgaaggcga tcttggcgat gcgcttgctg
    14641 gtggccagcg gcatgccggc ttcacggccg tagccattga cgatgttcag ggtgcccggg
    14701 ggcagcaggt cggcgatcag ctcggccagc accaggatgg agatcggggt ggattcggcg
    14761 ggcttcagga ccacgcagtt gccggcgccc agcgcggggg ccagcttcca ggcggccatc
    14821 aggatcggga agttccaggg gatgatctgg ccgacgacgc ccaggggctc atggatgtgg
    14881 taggcgacgg tgtcttcgtt gatctcggag atggtgcctt cctgggcgcg caggcagccg
    14941 gcgaagtagc ggaagtggtc gacggccagg gggatgtcgg cgttcagggt ttcgcggatc
    15001 ggcttgccgt tgtcgacagt ttcggcgtag gccagcttct cgaggttggc ttcgaggcgg
    15061 tcggcgatct tcagcaggat gttggagcgc tcggcggggg aggtcttgcc ccacttgtcg
    15121 gcggcggcgt gggcggcgtc gagggccagt tccacgtctt cggcctggga gcgggcgacc
    15181 tgggtgtagg gcttgccggt gatcggggtg atcacgtcga agtattcacc cttcagcggg
    15241 gcgacccact tgccaccgat gaagttgtcg taacgggact tgtattggac cttggcttcg
    15301 gcggtgccgg gcagtgcgta cagcatggaa atctcctcct cgagtggatt gataacgcgg
    15361 tgttgggcgt tcaatagaga tcagcaggcc gcgtgccata cggggagttg gggcggtaga
    15421 aaatgaattg ttcaatgtat tcatgaagtt gatgctattc atgttgcatt gcaacgtcct
    15481 tggagcctgt ggagcacctg ttcattttcg cgacaggtgc tccatcctgc gtgtgcaggc
    15541 gggacagcgc cttcccgcgc cgccagcccc atggatccat ggtgccggtg cgcgcgcggt
    15601 ggcatcatgg cggttcctgc atcgaccagc gtctccatgt cctcccagcc ttcggcggat
    15661 caggcccccg cggccgtcat cgaaccttcc ctgcccgact acctgcgcga aacctacacc
    15721 tgggcctatc tggacccgcg caccgtgccc tggctcgacc gtcccctggt ggtgtcggcg
    15781 atcctgtggg gcaacgcccg ccgcctgatg gatgccgccg tggcggaatt cactcccggc
    15841 cagcgggtgc tccaggcggc cgccgtgtat ggcgacttct ccgtgcgcct ggcgcgccag
    15901 ttggggccgg agggggcgct gaccgtggtg gatgtggcgc gcatccaggt ggccaacacc
    15961 cgtcgcaagc tcgaagcctg gcccacggcg cgggtgctgc gggcggatct caccgagccc
    16021 cagggcacca ccttcgacgc ggtgtgctgc ttcttcctgc tccacgaggt gccgccccac
    16081 gcccgccagc gcatcgtcgg caatctgctg gcggcggtgg agccgggcgg caagattgtc
    16141 ttcgtcgatt accaccggcc gcgctggtgg catccgatgg ggccggtgat ggcgctggtc
    16201 ttccgctttc tcgaacctta cgcgcgttcc ctgctggccg aatccatcga caccctggtg
    16261 ccggccgcgg ccgacttcga ctggcacagc gagacccgct tcggcgggct ctaccagaag
    16321 gtggtgggcg tacgccggcg ctgaagcgcc ggattcagcc gaggcggtcc agacaggccg
    16381 ccagcgcggg caggccttcg cggatcgccg ccacgccggg ggccagcagg atcgccgact
    16441 tcacctcgta gacatgaccg tcgcggaccg ccggcagggc gtcccagccc ggtcgggcga
    16501 gtacctgggc gggctggaag tgtttgccgc accaggagcc gacgatcact tccggcgccc
    16561 gcggaatcac cgcctccggg ccggagagga tgcgcgcctt cgcgccggca tgcaccgcca
    16621 gctccgcgaa gcagtcttcg cccccggcaa ggtggatcag ctccgacacc cagcggatgc
    16681 cgctgatgag gggatcgttc cattcctcga aatacacccg cggacgtcgc cctaggcggg
    16741 cgatgcgggc ctcgccggcg gcccgggcgg cggcgatgtc ggtctccagc cccgccacca
    16801 gggcgagggc ccgggcctcg gcgcccacca gccggccgac cgtctcgacc atggccagga
    16861 tgccgtccac gctgcgctgg ttgaagacat gcaccgcaac gcccgcccgc accagatccc
    16921 gcgcaatctc gccctgcagg tcggagaagg ccagcaccag gtccggttcg acggccagga
    16981 tgcggtcgat tttggcgctg gagaagccgc tgaccttggg cttttccttg cgcgcctggg
    17041 gcgggtgggt ggtgaagccg gagatgccgg cgatgcgggc cgcctcaccg agcagataga
    17101 gcacctcgac ggtctcggtg gacaggcaga cgatgcggca gggcagtcgg ggcatggcgg
    17161 acgtcgggtg ggggcggttc aggggcgcag atcggccggg aagggcgccg gacagtcgcg
    17221 catgcgcgtg gccggcgggc acaggcggac gagggacagg aagtaggcgc cggtcttcag
    17281 cacgtcggcg ctgtcgcggc gcaggctgtc cgcgtcggcg gcgggcaggc gccaggcgcg
    17341 ctgcaccacg taaaggatgt ccggaccctc gagggcctgc accagggcca gctcggcgta
    17401 gtcggtgccg ggcacctggg ggcagatcag cagggtgcgc agacgcgcgc gcccggtcgc
    17461 gtcgtcggcg gggagcgctt cgatgggctc gggcgctgcg ccgcagcggg tctgccgact
    17521 ggcctgcaga ccttcaagat agaggcgtgc ctgaccggtg gcggggcggc ggggcaggcc
    17581 ctgcacgctg atcaggtcgg tccattcggg caggcgcggg ccgacgggca ggaagtcggt
    17641 gcgccagtct tgaccgggga ggccgccgct ctggctggcg tagtgccagc cttcgggcag
    17701 gggcaggttg agccagcgcc gggggtcggc gcccgcgccc gcgtcccgtg ggtgggccgc
    17761 ggcgagttcg agcagccggc ggtagggcgg gtcgagttcg ccctggaagc ggccgccacc
    17821 gttttccgcc accgccacgc gggaggcgcc tggcgcgaag cggtaccaca cctcgtagct
    17881 gcgcgtcgcg ccgcaggccc gcacggtcca tcgttcgagg gcatggtgga tccgcgtcgg
    17941 gcgggtttcc gccaggtccg gcgcggcctg ttcggtgtcg gtgacgcgca cgtcggtgca
    18001 gggcttcgcg cgccggcctt ccagttccac gatgcgatcg cgtacccgct cctgactgtc
    18061 ggcccgccgc caggtaccgg cgtagccggg caccacgcgg gcgtcggcgg gcagcggggc
    18121 gagctggccg gggccgagga gctgttcgcc gggcagcggg ccgccctcgg cgcgggcgag
    18181 cgtcgcggcc agcaggagga gggcggcggt gcgcttcatc ggcgggcgcg ggcttcgtcg
    18241 agctggccgc acaggcgctc ggcgccgtcg aggatgcgcg gcgtgtggcg ctggatctgg
    18301 tccggcggga tgaagaacag gttgccccgg gccactgcgt tgagcttggt ccagcgccgc
    18361 cagtcgtcga gccactccgg ccgtgcctcg cccatgccgc tggcgacgat gacctccggc
    18421 gccgcggcga gcaccgcttc ggtgtcgatg gcgggcgcca gcacgggcag ccgcgcgaac
    18481 acgttctcgc cgccgcacag gcggatcacg tcggagatga ggtgctcgcc gttgatggtc
    18541 atcagcgggt ggttccagat ctcgtagaac acgcgcaccg tcggccgcgc cgaatagcgg
    18601 gccgccaggg cgccctggcg ggcgcggaag gcctgcgcgg cggcctccgc ggcggcttcg
    18661 gtaccggcca ggcggccgag ctgggtcagg ctgcgggcca cgtcctcgat gcgccgcggc
    18721 tcgttgatga agaccgggat gccgagggcg cgcagcttgt cgagatgggc gtcccggttg
    18781 ccgctcttcc aggccaccac caggtcgggc ttgagggcgg cgaccttttc catgtccacc
    18841 gcggtgtagc tgcccacccg cgccaccgac cgggcggcct cggggtagtc gctgtactgc
    18901 accgcgccca cgaccttgcc gccggcgcca gcggcgaaaa gcagctcggt gacgtggggc
    18961 gccagactga cgatgcgccg tgccggctgg gccaggtgca ccggcgtgcc ctggtcgtcg
    19021 gcgaggtcga tggcgccgcg ggcggcgccg gagacggtga gcagggcggt gagcagcagg
    19081 gcggggaagc gtcggcgcat ggcgggggtc agagtcgggc gagggccgcg gcgaggcggt
    19141 cccagtgggc aggtgtaccc ggcaggccga agcgcaggcc ggtcggcgcg gtgaagaggc
    19201 gaagcaggat accctggcgg gccagggcct cgtggatttg ggctgcatcg ccatggggca
    19261 cccactggaa cagggcggtg ccagcggcgg gaggcagccc gtgggcggcg agcagcccgg
    19321 ccagacgccg gcagtcggcg gccaggcggt ggcgggtggc ggtctgccag gcgcgatcct
    19381 gcagggcggc cgtggcgacg gcgcgggcgg gcccggcgac ggtccacggt ccgaggcgct
    19441 cggccagggc gtcgcgcagg gctgcctcgg cgaacacgaa gccgacccgc gcgccggcca
    19501 ggccgaagaa tttgcccagg gagcgcagca ccaccaggcc gggcgtgccg gcaaggggcg
    19561 tcaggctgtc gaccgggtcg gcatcggcga aggcttcgtc cacgatcagg cggccgccgc
    19621 gggcctgcag gcgggcgtgc caggcgagca ggcgggtgcg gggaaagcgc tggccatccg
    19681 ggttgttggg ctggacgagg aggagggtgt ccgtacggtc gacggcgtcg tcgtcgaggg
    19741 ccgcggcggg cagcgccgtg accgcgtggt ggcgccaggc gtggggatgc tccgcgtagg
    19801 tgggcgcgag ggtcagcacc cgccccggcg gaaagcacgc cggcagggcc tggagggcgg
    19861 cctgggagcc ggccaccggg agcagctcgg cgctgccgta atacgccgcg gcagcggctt
    19921 cgaggccgtc gtcgacttcc ggcaggcgct gccacgcctc ggcggcgagg gacggcaccg
    19981 ggtagccgtg gggattgatg ccggtggaca ggtcgagcca gtcggctcgc gcgatgccgg
    20041 tgcggcgcgc cgcctcgcgc agccggccgc cgtgttcaag cacggagggc gaatccggcc
    20101 gcggcgatca cgccgagcca cagccaggtg gtgttctgca cgagggccac ggcccggtcg
    20161 atgtcggcgg cgcgggcacg ggcgccgtca ccgagggccg ggcgggtctc cagatggccg
    20221 tgatagaccg ccgggccgcc caggcgcagg tccagggcgc cggcgcctga ggccatcacc
    20281 gggccggcgt tggggctgtc ccaggcgggc gcctgggtgc gccagcagcg cagggcggtg
    20341 gcagtgtgcc cgagcagggc gtaggacagg gcggtgaggc gggccggcag ataattgagc
    20401 acgtcgtcga tgcgcgcggc ggcccagccg aagcgcaggt agcgcgcgtt gcggtagccc
    20461 cacatggcgt ccagggtgtt ggccaggcgg tacagcaggg cgcccggtcc gcccagcagg
    20521 acgaaccaga agagggcgcc gaacaccgcg tcgttgccgt tctcgaggac cgattcgacg
    20581 gtggcgttgg ccacgccgga ttcgtcgagc tgggtggtgt cgcgggagac catccaggac
    20641 aggcgctggc gcgcgccgcc caggtcgcca tcgagcaggg gcgtcgccac ggccgcagcg
    20701 tgctcggcga ggctgcgtcc gccgacggcg agatacagca gggtggcgtc cagcagggcg
    20761 cccgcccagg gatggagcgg ccgcagcgag cccgccagcg cgacccaggg cagcaccgcc
    20821 agcgcccagg ccagcaggcc cgcggcgcga ccggagccgg tgaggcgcaa caggctttcg
    20881 atgcggccgg caatgcggcc aaaggcgaca agggggtgat ggtgccgggg ttcgccgagg
    20941 aggcggtcga ggagcagacc gagggccatc aacagggcca ggggaagcgc cacgggtgac
    21001 gtggcgaccg ggagggtgaa gccgggcatg ggataatcga caacttttcg cagacgggat
    21061 tgtaagccat gcgacgcgcg tccaccctga tggttcaggg caccacctcc gatgcaggca
    21121 agagcaccct ggtggcgggc ctggcccgcc tgctgcgccg ccgtggcgtg gcggtcgcgc
    21181 cgttcaagcc ccagaacatg gccctcaact cggcggtgac ggcggacggc ggcgagattg
    21241 gtcgcgccca ggcgctgcag gcgctggctg ccggcgtccc gccccatacg gacatgaacc
    21301 cggtgctcct caaacccgcc agcgacatcg gcgcccaggt gatcatccat ggccgtgccg
    21361 tggcccatct gtcggcgcgg gattatcacg acttcaagcc gcgggcgatg gccgcggtgc
    21421 tggcgagctt tggccgcctc tgcgcgggct acgacgcggt cctcatcgaa ggggccggca
    21481 gcccggcgga gatcaacctg cgcgaccggg acatcgccaa catgggcttc gccgaggccg
    21541 ccgactgccc ggtgatcctt gtcgcagaca tcgaccgggg cggcgtcttc gcgcacctgg
    21601 tgggcaccct ggacctcctc tcggccagcg agcaggcgcg ggtgaagggt tttgtcatca
    21661 accgcttccg tggcgacatc ggcctgctcc agtccgggct ggactggctc gaggcgcgca
    21721 ccgggcgccc ggtgctgggc gtgctcccct atctgcacgg cctcttcctg gatgcggagg
    21781 atgccctcgg cgaggcctgt gtcgacaagt cggccacgcg cctgcgggtc gtcgcgccgg
    21841 tgtacccgcg catctccaac cacaccgacc tggacgccct gcgcctgcat ccccaggtgg
    21901 acttccgctg ggtcgggccg ggccaggcaa tgccccccgc cgacctgatc gtgctgcccg
    21961 gctccaaggc cgtgcaggcc gatctggcct ggctgcgcgc ccaggggtgg gagcccgccc
    22021 tgcgccgcca cctgcgctat ggcggcaagc tcgtggcggt gtgtggcggc tttcagatgc
    22081 tcggcacccg gctgcacgac ccgcagggcc tggagggcga ggcgggcagc gtcgccggcc
    22141 tcggactgct cgatgcagag accgtgctcg aagcggagaa gcagctggtg aacgtccacg
    22201 gtcggctcgc cctgcccggc gccgcgccgg tgagcggcta cgagatccac atgggcgtga
    22261 cccgcggtcc ggccctggcg caggcggccg tggagctgga ggacgggcgc cgggacggtg
    22321 ccatctcgga cgatgggcag atcctcggca cctacctgca cgggctcttc gatcacccgg
    22381 acgcactggt ggcgctgctg gcctgggcgg gggcgggcga cgtggatcgg gtggatctgg
    22441 cggctcgacg cgaggccgat ctggagcggc tggccgacgc gctcgaggcc catctggatc
    22501 tgccgcggct ggccgcatgc ctgggtgacg ggacggccgc cgagggcgtg cgtacgtcat
    22561 tgcatgtgcc ccttgcttga ccgcgtttcg ggccctctct aacattcgcc gcatggacgc
    22621 cgaattcgcc tctcttgaac agaagctcga gcagcttctc gccctgcatg ccgccgcaca
    22681 ggcggaaaac cgcagtctgc gggagcggct ggctgccgtc gaaggcgcca accgcgagct
    22741 gaatgaccgg ctgggtatcg ccggatcccg cctcgaagcc cttctcgccc gcatccccga
    22801 ggtctgatgc atggaacacc tggatatcaa actcctcggc cgcgaatatc gcgtggcctg
    22861 caagcccgaa gagcgggacg gcctgatggc ggcggtggcc tatctggacg aaaagatgcg
    22921 cgatctgggt gccaagacca actcggccgg cgagcgtctg gcggtgatga cggccctcaa
    22981 catcacccat gaatttctgc agttccagcg cgccggcgga tttgacatcc aggcggtcaa
    23041 gcgtagaatc gagcacatga acagtcgcct tgatggcgtc ctggcgcctc aggaaaagct
    23101 cttctgaggc aaccggaagc gttgttcaat gttttgattt gcaagggttt tcaggttcga
    23161 tcatcccctg cggtgttggt caaggctgta tactccttga accaatgtct ttatgactgg
    23221 gtcgtggccc gtagaaacca ctggtgtgcg cgccccactg tcgggtgtgc ctgaagtggg
    23281 aggaaagcga cccccctgaa ccccggttca ggatgccggc ctgaccgcac aggcggggga
    23341 cctacaaaaa aagcgcagag tttcggctct gcgctttttc tttgtctgag cacgcgggca
    23401 actggggctg ccgcgcgctt cggtggcctg ttccacagga aacaggccac cggcagagac
    23461 tgcttattgc ggttccttga aaccggcgcc cgcctggttg gcgaggaagg ccacggcgcg
    23521 ggtcagttcg gtgtcggtca gatccgccgc gccacccttg ggcggcatgg cgttcttgcc
    23581 gttggtggcg gacttcacca gaccggccag accggcgccc agacgcggac cccaggcggc
    23641 cttgtcgccg gccttgggcg cgccagcggc gccgctggcg tgacaggcgc tgcacacggc
    23701 cttgtagatg tcttcgccgc tacggctgcc cggcgcggcg gccggcgcag cggtcaggac
    23761 cacgctggcc acgggctggg tcagcttggc agccagttct tcatcgacgg cggccttgtt
    23821 gccgcagccg gccatctggg tggccagcgc agcgacgcca agggtgagaa caagccgcac
    23881 cgggtggcgg gcgggagcag agcgaagcag atccgacatg ctgaaatcct cgagagcggg
    23941 gggacgattg attaaagcgt tgattatagc gggggtattc cccttctgtc aggcgaagca
    24001 tggacgccgg cggcgattcg cgctatcctg cgcgccgcgt gacgtctccg gcgtcattgc
    24061 tccgcgcgcc cgtagctcag ctggatagag tactgccctc cgaaggcagg ggtcggacgt
    24121 tcgaatcgtc tcgggcgcgc cattctctca atgggttgga tcgaaagatc cggcccattg
    24181 tcgtttccgg cgtccgatcc cgcggcgcgg gctgcccgcg cggatgcccg tctccgaacg
    24241 ccgcgtgaag ccgtcggtcc cgtactgctc gtggcagggg tccttgatca atggcttgcg
    24301 gtggcagggc agcctgcggc gtcttcgatg aaggcgagca gatcgtccgt ctggaaggac
    24361 ttgtcgaaga aggcgcgtgc gcccagcatc cggcagcggg cctggacgac ggagtgggcg
    24421 tggttggaga agaccaccgc ctgtggcgag ccgaagtggt ccggctggcg ctgcagcgcc
    24481 gccagcacac ggagtccggt gccctcccgc agttccagat cgacgatgac gagccggggg
    24541 cgggtggcct gaagcagctt caggccttcg gcctcgctgg cggcggcacc gacgaggcgg
    24601 atgttcggga tgtcggccag catggccgac agcaggtcgc gcaggacggt ggagtcttcg
    24661 atcagtgcga tgtcgaaatc ggtgtgggca ggggcaggca tgggggttct cgtgcggcgg
    24721 aggtgttcag ttcgggcgtg gcgcatgggt cggcgcgaag gcgaaagtgt ccgggagggt
    24781 ctttgacgtc caggttgagc gttcagccgg gcggtcggct tcggccgacg ggtcacgcac
    24841 gaagcgcagt agcgccccgg tgggggcccc tcaggccatg acgacccggc ccgccacaca
    24901 acatccccgc gggccggtcc gtgtctgggg cgccggcatg ggtgagatag accgtcggcg
    24961 tcaatgggag gtgtctctgg gcagcaggac gcaatcggtg acggaggtcc aggccgcggg
    25021 tccgatcagg cgatcgacgt cgtgccggtc gggcaggcat gaggtgctct ccaggcgcgg
    25081 ccgcgcgccc tgatggccga cggacgaccc gtacagcatg ccggcgcaga gggcgacgga
    25141 gctgacgagg gacagtcgca ccgaccagtc ccatttgccg gggagcgccc acttgggttc
    25201 ttgcggggtg ttctggatgc cgcgcatgtt catctcctga gggggtggtt caggtgccgg
    25261 gatcatcggt ggccacgccg gcacgcgggg tcggatctgt gtccgatcgg ctgtcctgct
    25321 ggcgtcccgt tcggccgaaa ccggatcggt gacgccgcac gcccttcgag gcgaagctgg
    25381 ccactgcgat ctgaccggac cgcaacgcag gggttgcagc tgcactgcgt aaagtcatga
    25441 caagcggtgt aaagaaggcc gccggcggag cggacagggc accgccgggc gggcccttgc
    25501 ggggccgggg cacgggtcgg gcgtgggcga catggaggcc gtgtcccggc gtcgttcaga
    25561 accggcggcc caggagggcg ccaatcagca gaccgaccac cccgacgacg gcggccgact
    25621 gcagcggctt gcggcggatg taggcgcctg cggcctcgct ggcgtgggcg gcgtcgcggc
    25681 ggacgatgcc cacggtgtcg cgggcggccg tgcgcagatt gcccatcagg ctggtggccg
    25741 cctcgcgggt ggtgtcggcg gtgattccga gctgcttgcg gaccgggggc agggcgtcgc
    25801 gggcgctgtc tcccaggtcg ttggcgatct ggcgcaggct gttggcagtg gaatgggtgg
    25861 cgctcatggt ggtgctcctt ggggtgggga tcgggggcgg gcggaggtgt cgcgaccgcg
    25921 caatcagttg atgaggatga tgtcgttctg gaccgccttc acgccgggga tcgtccaggc
    25981 gagtgcctcg gcgcgggttt tctcggcggg ggagggggtg aagccggaca gctgcacgac
    26041 gccgttgcgg gtttcgacgc cgatggcggt gatgcgtacc gaccggtctt ccgcgtgcct
    26101 ggccttgatg ttcgcggtga tggaggcgtc gtcgatgaag cgggcggccg ccgagcgggg
    26161 ggcctggcgg tcgccctggg ccgcggtggc ggcctgggtg gcgggtacgc tggcgagcgc
    26221 cagggcgagg aggctggcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    26341 gtggcggcct gggtggcggg tacgctggcg agcgccaggg cgaggaggct ggcgaggagg
    26401 atgccgggga ggggtgtctt cacggtgggg tctccttgtg gaccggaata gccgggttcg
    26461 gggcgggcac cggaccgggg gtccgacacc gttctgccat gactgcactg tacggaaggc
    26521 cgggcgggcg gactgtccga caagcgcgga tgcgtgtgta ggaatcggcc gtcggacatg
    26581 tcggcgctgg ccgggccggg gacgccgccg gcgggccgcg tggtcgtgcc ccgtggcggc
    26641 aggggcacgg cgcggtgcag gaaaattccg acgcggggct catccgccac cgacaccggg
    26701 gcgggcgcga cggacctaga atgggtccgg acccttacac gcttccatca tgaacatgcc
    26761 gatctctccg cgctcccgcc gtcgcctgcg ccatgtggcg gccatcctcg ccgtggcggg
    26821 gctgggtgtc gcgctgttcg acgggaacct gctgcgcggc cccctggccc gcttggccag
    26881 cgcgcagctc ggtcgcagcg tccagatcgc aggcccggtc gatctgcatc cgtggagcct
    26941 tcacccgacc ctcagtgtgg gcggcgtgac cgtggccaac gtgaccggcg gccgcgccag
    27001 cctcctggcg agcgccggcc ggctggccgt gacggccgac ctgcgggcgc tgctgcgggg
    27061 ggcggtggtg ctggatgcgc tggaggtcga ccgggccgag atccacctgg aagaagaccg
    27121 ggaggggcgc ggcaactggc agctcggcgg gcagatcggc gcgggcggca aggaccccgc
    27181 tgcggcgccg acgcgcctgc gcatcgcccg ggtgcgcatc cacgacagcc ggttgagcgc
    27241 cgagctgccg tcgcgcaaga cctcgctgca gctcgaggtg gcgacagggg acgacggtcg
    27301 catccgcttc accgcccgtg gtcgctacca ggggcaggac ctgacggtgg acggcgaggc
    27361 ggggagcgtg cccgggctga tcgacggcca gctgccctac ccgctgaagg ccgccggcac
    27421 ggtgggcgcc acgcgcttcc gtatcgacgg gtcgagcgcc gatctggcag ggctgaacgg
    27481 cctcgacgtg ggcttcgagc tggcgggccg cagtctggcg gagctgtatc cgctgaccgg
    27541 cgtgccgctg ccggcgtctc cggcctaccg gttcgcggcg cggctggtgc agtccggcac
    27601 cctgtggcgc ttcgagaaca tcgacggcac cgtcggccgc agcgatctgg cgggcacctt
    27661 tgccgtggat cgggggcccg tgccgcagcg cctgagcggc acgctgcgcg cccggcatct
    27721 ggatctgcag gatctctcgg ggttcctcgg cgcgcgcgac agcagcggcg agcggctggc
    27781 gccccggccg ggcaaggtgt tgccggcacg cccgctggga ttcgacaaga tcgctgcggc
    27841 cgatgtggat ctggacttcg ccattggcga catccgccac agcggcctgc ccctcgatgc
    27901 cgcgtcgggg catctgcgca tcgtcgatag gcgtgtcctg ctccagcccc tgcgcgtcgg
    27961 catggcccag ggcgaggtga gcggcgtcct cgagctcgag acccgcggca tgcccgcccg
    28021 tgcgcgtctg gatctgcagg ccagccggct gaagctgcgt gagctgatgc ccacgctcga
    28081 atccagatcc ctgaccaccg gcgtcctcgg gggccgggcc acggtgtcca tgcggggcaa
    28141 ttccgtcgct gcgctgctgg gcagcgccac cggcgacctc gccctcgcca tggatggcgg
    28201 cagcaccagc cgcctgctgg tgcgcctggc caatctggat gtggccaacg cctttgtgtc
    28261 ctggctggcc ggcggtcagc gggaggacat ccgctgtctg gtgggagatt tcagcgcccg
    28321 cgacggggtg ctcgagccgc gcgcgctgtt gctcgacacg gagcgcaccc tcatccgcgg
    28381 cgaagggcag atcaatctgc gcgatgaaac gctggatctc cgcctgcggg cgtcggcacg
    28441 ggacggcagc ctgctgtccc tgcgggggcc tctgcatctg gacggaagtt ttgccgaccc
    28501 caaggttctg ccggagccgg tcccgctggg cagccgggtg gccggggcgg tggcgctcgg
    28561 gctgatctcg ccgccgctgg ccctgctgcc cctgctggaa gccggcgatg cacgcgagtc
    28621 ggcctgcaag cgtctcctcc agcaggccgg gaagacgatg gggcgaccct gaggggccgc
    28681 agcagatcgg ggcgtgaacg ggcgccgggg cccgcgggac gacgttgtgc cgtcttccgg
    28741 aggggcccgg cggaagtgcg gcgggcgctc atcgccgccc gcgcgcattc atcccttgcc
    28801 cagcagcagg ttgaagacaa agctgacgat ggcaaggatc aggaagatga agaacaggac
    28861 cttcgcgatt tccaccgcgc cggcggcaat cccgccaaaa ccgaacaggg cggcgacgat
    28921 cgcgatgaca aaaaagacca cggcatagtg cagcatggcg gttctcctga aacggtgatg
    28981 acggacgggg gcgatggcgc ggcgcggggg ctcaggcgtc gagcatcttt tcccaggcct
    29041 gcacgtggcg cttggcttcg tccagggtga ggccatagcg ctcctggatc cggccggcga
    29101 gtacctcgcg gcggccttcg atgacgtcca ggtcgtcgtc ggtgagcttg ccccagtgtt
    29161 ccttgagctt gcccttgagc tgcttccagt tgccttcgat gatgttgtgg ttcatggcga
    29221 ttctcctgag gttgagggct tcgagcgttc cggaggggtg tcccgggcgg tcggcatgcg
    29281 gtggagcctg catgactcca gcttaggcag gcgccgccgg gcgggcagtc ggctggatct
    29341 gaacgggatg tgggactgcg ccgacggccc tgtcgggcat cgccgtccgg ggtgtcccgg
    29401 tggcgcccgg cggggcccgc cggggcggcg gagcgcggcc ccgcagcggc gggaaggcgt
    29461 gtcagacaac tcccacatac cattcagcgc cgaccgacgg tatcgtgatg tttgtcacgg
    29521 tacagtttca aaaaaggcgt tttatacagc gcccccaccc ggcagcacgc ccccgaccca
    29581 tcgacaggag cacaggatga aacgcgtcag catcgactgg aatgccaaac ccgtccgcgc
    29641 agcccgccgg agccctgcgc gtagtgtgtg gaccgacacc ctcgaggacg agatcgatct
    29701 gcgcaggctg cggaccgaac gcagactcga caaggtgcgc cgcgccctca tcaaccagag
    29761 cggcctgacg tcccgctgat cggcaggccg ccccccgacg gggcggggcc gcccgcctgt
    29821 ccgcaatgcc ccgcccgttc cccgccgcca tgaataccgt cctgcccctg cgggatcccc
    29881 gggcccgcat gcgcctcgtg cggcattggc ccgtcttcct ggtgatcctg ggggtcgggc
    29941 tgctcctcgg gctgctcacc atcggccggc tccactcgga gttcggcgtg agcgccctgc
    30001 gcacctcgca ggcctacaac agtcagatcg accggctgaa ccggctgctg atcgtgctgc
    30061 tggatgccga gaccggcgtg cgcggattca tcgcgagcgg gctcaatccg gtctatctcg
    30121 agccctacca gcaggccgaa caggacatcg gccccttgct gaaagccatc tccaatgact
    30181 atccccaggg cacccaggac cacaccgagt ttgagctgct cgggcagctg atcgccgtca
    30241 agcgccagct gctggcggaa acggtccgca tccggcgtgt gccgacggtc gtgccgccgg
    30301 gagagaccgg gccgggcaag gcctacatgg accagatccg cctttccatc gccgtgctgc
    30361 gggggcgtct ggaagagcgc aatgcccgct cgattgccga atccaccaag cgcttcaacg
    30421 acacgcgcac cctcgtcacc gtactggccc tgggtgcggt ggggctggtg ctggccctgt
    30481 ttgccgtgca gcaacggcag accgtcctgc gggcgcgcat tgccgagctg ctggagcggg
    30541 agaacatcgc tctggaatcc acggtggccg agcgcacgcg ggagctgtcg gatctggcga
    30601 gctacctgac caccgcgcgg gaactcgaac aggcgcggct ggcccgcgag ctgcacgacg
    30661 aactgggggc gctcctcacc gcggccaaga tggacgcgag ctggttgctg cgcagcctgg
    30721 gcgcgcagtc cacgccggac atccaggccc gtttccggcg cctgatcgac accatcggtt
    30781 cgggcatcac catcaagcgg cgtgtggtga gcgacctgcg tccgccgctg ctccaggggc
    30841 tgggcctggt ggaagccctg cgagccctgt ccgacgatct ccacgacgag taccgggtca
    30901 cgctgcagct gcccggcgag aacatcgact gcgacgagga acaggccctg gccctcttcc
    30961 gcatcgtcca ggaaagcgtg accaacatcc gcaagtacgc gcgggccagg gccatcgagg
    31021 tgggtctggc ggtcgacgcg ggggtcgtcc ggctatgggt gaaggacgac ggtgtcggct
    31081 tcgaccccga caacccgcag ctcaatcgcc atggcctggc cgggatgaag caccgggtgc
    31141 agatgttcga ggggcgtttt acggtgacgt ctgcacccgg cgccggaacg cgcatcgacg
    31201 ccacgatgcc cctgcggccg gcggccccgt cggcgcacgg ctgaatccga cgcacccacg
    31261 ccggcggcgg gctccggcgt ggcgcggacc cgcccgtgcc ggcggctcag tcgacgatca
    31321 gaccgtggcg cagggcgtag gcggcgagtt ccgcattgct ggccacgtcg agcttttcca
    31381 ggaggcggga gcgataggtg ctgaccgtct tcacgctcag attgagcacg tcggcgatac
    31441 cggacacgga ttcccccctg gcgaggcgca ggaagacctg cagctcccgc tcggagagac
    31501 tctcgtgggg cagtgcggtg cccgcgccgg ccagatcgtc cgccagcaat tcggcggtgc
    31561 gcggtgacac atagcgccga ccctgcgcga ccgtgcggat cgcgcggatc agctcatccc
    31621 gttcgcagtc cttgcacagg tagccgttgg cgccgttgcg gatcatgggc agcgcatagc
    31681 gctcctcggg gaagccgctc agcaccagca ccttgagatc cgcgtagcgc tgccggacgg
    31741 cccgcagcac atccacgccg ctctgccccg gcaaggacag atccagcagc aggacatcgc
    31801 agggcgtcgt gcgcaggtgc tgcagggctt cctcgccgga ggcggcctcg aactccacct
    31861 ggatgcccag ttcatcggag agcatttcgc ggaatcccgc acgcacgatc ttgtggtcgt
    31921 cgacgatggc cagacgcagc atggcggttc tcccggacgg aatggacgga ttgtatcggt
    31981 ggggcgcgcg gcggcgcgtc ccccgagtcc gacaatcatg caggaattgt cctacgggcg
    32041 ggcgccgatt cccgtgcggc aggggcgccc gcatgtaggt gccggcctac aggccaatcg
    32101 gcggtggatg actgcgcgcc aggccgggac gggggaggat gcaagcatca ccgtcgcccg
    32161 tccatcgctg gaatgccgcc atgaagatcg tccgccgtcg tccccttgcc ctggaagcgt
    32221 ccgtccggat cggcctgctg gccgtcacct tcgttgccgc gtttgccgcg ggacattcga
    32281 ccgcgtcccg cgcgccgcag tcgctggatg acccggccgc ggggcccgcc ctgctgcgcg
    32341 acggggtcga tcgggccggc cggagcgttg ccgcggccgt cggcctcgac gtgtattccg
    32401 aggcgggccg gcccgtcggg cgcctgaccg ccctcctgca cggcgaaggc cacgatggcc
    32461 tggcccggat cgccgtggcc gaacggggcg acgaaggcgc acgggagatc gtggtgccgc
    32521 tcggccgcct ggtctggcgg gacgatcgcg tggtgctggc ggacggcgcc gcgccgcgct
    32581 ggctgatctg aggcggcgca agtggccacc gcggcggcgc gcccgcagga ccgcgcgacc
    32641 cgcctgaagg tccgccgggc acgcaagatg gcccgctcgg cccacgccta cgtgcggggc
    32701 agcacagccc gtttttacgc ctggctggcg gcgagcgacg gcgacgccct gccggacggt
    32761 ccgcccgtct ggatctgcgg tgactgccac ctgggcaacc tggggccggt ggccgacagc
    32821 gatgggcgta ccggtgtcga gatccgcgac ctcgatcagg cggtgatcgg caacccggcc
    32881 catgatctcg tccgcctcgg gctgtccctg gcctgcgcct gtcgcagcgc cgacctgccc
    32941 ggggtcatga gtctccgtgt cgtcgatatg ctcctggacg ggtatgcccg tgccttcgcg
    33001 cagcgtccgc gtccaccgtc gccgccggac agcgtgaagg cggtgatccg ccgggccgtg
    33061 aagcgtacgt ggaagcagct gatgcgcgac cacctcgacc acgcccgtcc gcggatcccc
    33121 ctcggcgccc gcttctggcc gctcacccgc cgcgagcagg gggccatcgc acagtgtctg
    33181 gacgagaccc gcctgcccga actggcgcgc cagctgggca tcgcgcggga caccgaggcg
    33241 gtgcgcgtgg ccgacgcggc ctattgggtc aagggctgca gcagcctcgg gcggccccgc
    33301 tacgtcgtcc tgcttcaggt ggacggcgcg gccgaggcgc gtctggcgat ggtggacatc
    33361 aaggatgcgc cgcccgccgc ggcaccccgc accgggtcgg tgagcatgcc ccgggacaac
    33421 gccgagcggg tggtggaggc ggcccgtgcc ctggcgccgg cgctgggcgg gcgcatggtg
    33481 gcgatgcgtc tgctcgaccg gccgatggtg gcgcgcgagc tgtcgcccca ggacctcaag
    33541 atcgagccgg accagctggc cccggcagac atggcggccc tggcgcacta cctggggcgc
    33601 atcgtcggcg atgcccacgc ccgtcagatg tcggccccgc agcgtcgtca ctggcatgcc
    33661 actctgcgcc gttcccacag ccgcgtcgtc gacgccccgt cgtggctgtg gcgcgccatc
    33721 gtggacgccc tggcggatca tgagcggggc tatctggaac attgccggcg gcacgtcgca
    33781 gcgctcgtcg cgtgagggtc gagcatcccg ggcgcagccc cgcgccctgt aggaactgac
    33841 cgacagaccc gccccggcgg gcgccctata ctggattcgt ccggaagcgc gcaggccgcc
    33901 gccgcgcgca gccggcccgc accctccgtc acgcgacgct tctgaacgcc atcttctgcc
    33961 cacgcgccca ccatgccgtc tgaacccacc gtcctgcgaa cggtcatcat cgaggacaac
    34021 gccgtgctcc tcgacttgct gacgggcatg ctggcgggta tcgaaggggt gagcgtggtg
    34081 gggcacgcgg cgagtgaggc cgaggccctg gcgctggtgc gcgccacccg gccccagctg
    34141 gccatcgtgg atctggagct gcgcggcagt tctggtctgg gcgtgctcgc cgcctttcag
    34201 cagacgcctg acgagtacgg cgcgccgctg gccatcgtgt tctccaacca cgcccatccc
    34261 atcgtgcagt cccgctgcct ggcgctgggt gcgcgggcgt tcttcgacaa atccttccag
    34321 atggacgatc tgctcgactt cgtgcaggcc acggcgcgcg ccctctgagg gcgctccgtt
    34381 cgtccgcctg cgccatcggg cgccagcggc gttcacgacg cagggggcgg aagcccgccc
    34441 cctgctgccg ctgtgcgtgc gccttacggg cgcacgacga tgtcgttctt caccatcgac
    34501 acgccgggcg tgccgcgcgc cagggattcg gcccgatccc gctccgtggt gctcttcgcg
    34561 aagccggaca gctgcaccgt ccccttgagc gtttccaccc cgatggccat cgcgctcacc
    34621 gtgctgtcct cggcgaactt ggccttgacg cgggtcgtga tggtggcgtc gtccacgtac
    34681 tgtccgacgg tggattgttc gcgggtgatg gcgcagcccg ccgcggaaaa ggcgagcaga
    34741 ccggcaaggg caagggtgac ggcggtggtt ttcatgacag gctcctcatc tggggaaagt
    34801 cggtccggta gacatgtgct ccggtacgta ctgctgactc cagcataggg cggggccacg
    34861 ccggcgcctg tccgacggga cggattgacg tgtaggactc gtccttcccg gcgccccggc
    34921 gcctacggcc acggtgccgc cgcgcgcgcc gccatccggc tgaaggccgg gcgcgcgcct
    34981 gtggcgccca gtgcggcgcg gagcgtgcca tgcacggggc atttgctgcg cagcatctaa
    35041 accattgtct aatttctgga caggccgatt ccccctaagg tgtctccaca cggtcgatcc
    35101 gctgggcgcg gaggaccgag ggggagcttg cagggctcct gcatcccgac atgaaggaga
    35161 caagaatggc cttactcaat cggatggact ggtacgacct ggcccgatcc accaactgga
    35221 ccccgaagta cgtcaccgag gacgaactgt tcccgcccaa gctgtcgggc gagttcggcc
    35281 tgccgctcac ggcctgggag tcctacgacg aaccctacaa gcagacctac ccggaatacg
    35341 tgaaggtgca gcgcgagaag gatgccggcg cctactcggt gaaggccgcc ctggagcgca
    35401 gccgcatctt cgagaacgcc gatccgggct ggaagtcggt gatgaaggcc cactacggcg
    35461 ccatcgcccg tggcgagtac gccgccgcca gcgccgaggc gcgcatgatg cgcttctcca
    35521 aggcaccggg gatgcgcaac atgtccacgc tgggctgcat ggacgagatc cgccacggcc
    35581 agatgcagct ctacttcccc cacgagcacg tggccaagga ccggcagatg gactgggcct
    35641 tcaaggccta cgacaccaat gactgggcca tgatcgccgc ccggcatttc ttcgacgaca
    35701 tcatgatgac ccgggacgcc atcagcgtct ccatcatgct gaccttcagc ttcgagaccg
    35761 gcttcaccaa catgcagttc ctggggctgg cggcggacgc ggcggaagcc ggcgaccaca
    35821 ccttcgccaa cctcatctcc agcatccaga ccgacgagtc ccgccacgcc cagatcggcg
    35881 gcccggccct caaggtgctc atcgagaacg gccagaaggc cgaggcccag aagcgcgtgg
    35941 acatcgccgt atggggcgcc tggaagctct tctcggtgct gaccggtccg atcatggact
    36001 actacacgcc gctggagcac cgcaagcagt ccttcaagga gttcatggag gagtggatcg
    36061 tcgcccagtt tgagcgcgcc ctcaccgaca tgggcctgga gctgccctgg tactgggaga
    36121 tcttcctcaa ggatctctcc gagacccacc acggcatgca cctgggctcc tactactggc
    36181 gccccaccgt gtggtggaac cccgctgcgg gggtcacccc ggacgagcgg cagtggctgg
    36241 aagagaagta ccccggctgg aacgacacct ggggccagtg ctgggacgtg atcatcgaca
    36301 acgtggtgga cggcaacatg gccaaggcct acccggagac gctgccctac gtctgcaaca
    36361 tgtgccagct ccccatcctc ggcacgccgg gcaagggctg gaacgtcaag gattacccgc
    36421 tggaatacaa gggccgcctg taccacttcg gctccgaggt ggatcgctgg gtgttccagc
    36481 aggagcccga gcgctacgcc ggccacctgt ccatcgtcga tcgcttcctg gccggaatga
    36541 tccagcccat gaacctggaa ggcgccctcg cctacatgaa catcgccccc ggcgaatgcg
    36601 gcgacgatgc ccacaactac gcctgggccg aggtctaccg cgccctgcgc gccgcccgca
    36661 aggccggctg agcccgacac cgccggcccc gcgcgggccg gcggctcaca tcaaggagat
    36721 tcgaagcatg gccttatttc cattgacctc caatttcgag ggggatttcg tcctgcaact
    36781 gttgccggtg gataccgagc acaccatgga cgaggtggct gccgccgctg cggtgcattc
    36841 cgtcggccgc cgcgtccggg cccgtccggg tcacatcctg cgggtccgcc gccacggcag
    36901 cgcggagttc ctgccgcgca ccctgaaggt cggcgaggcg ggcttccagc ccaccgagac
    36961 cgtggacatc atctggcagg ccgcctgacg gccaccgcga tcggagcgag cgcatgtgct
    37021 tcaccaaggt atgcaccctc gatgacgtgt gggaaggcga gatggcggcc ttcaccgtca
    37081 atggacacga gatcctcctc gtctgccccg agggcggcga aatcaaggcc ttccagggcg
    37141 tctgtccgca ccaggacatc gccctcgccg aaggcaagtt cgacggcaag cgggtgatct
    37201 gccgcgccca tctgtggcag ttcgacgccg ccaccggcca gggcgtcaat cccgacgact
    37261 gcgccctggc ggtctacccc gtcaaggtcg agggcgacga cgtgctggtc agcaccgaag
    37321 gcgtcgagcc cctgttcgcc cacacctgag ccggaccttg cggacgccgc ccggcgcggc
    37381 gtccccggca tcccacaagg agacaccatg agtacagcag cagcgggtca ggcctatcac
    37441 aacaacctgg tcggtccggt catgcgggcc ggcgatctgg ccatggcggt catcgaggcc
    37501 gccaaggtgg acaaccccgg caaggacatc tacgtggatg acaagcgcgc ctacatccgc
    37561 atccacgccg agcaggaaat gatcctgcgc caggccacca ttgaagagga gctgggccgg
    37621 cccttccgca tgaacgacct ggaggtggac ctcagctcct tcgccggcca gatcgagagc
    37681 cgcgacgact cggtgcgctt ctacttcgtc aagaagctct gagcgcccgc cccgtccacg
    37741 cccaaaaaga cacgaggaga cacccatgtc cgagattcaa gtcctgaagc cccagaagac
    37801 ctggagccat ctggcggtgc gccgccgcaa gcccagcgaa tacgagatcg tcagcgccaa
    37861 cctgcactac aacgaccgcg acccgaacgc gccctacgag ctggatccgg gcatgttcat
    37921 gaacgagtgg tacaagaagc acaccttcgg caccgccctc aagcacgccg actggaacgc
    37981 cttccgcgat ccggacgagg tggtctaccg cacctacaac atgatgcagg acggccagga
    38041 gacctacgtc ttcggcctct tcgaccagtt caacgagcgt gagcacgaca aggccctcga
    38101 tccccgctgg gccggcaccc tggcgcgtct gtatacgccg gcccgctacc tcttccacac
    38161 cctgcagatg gcctccgcct acgtggggca ggtctcgccc gccagcacga tcaccaactg
    38221 ccactacttc cagatggccg acagcctgcg ctggctgagc cacaccgctt accggaccac
    38281 cgagctggcg cgcgccttcg ccgacaaggg cttcggtcgc gacgaacggc actattggga
    38341 agaggacgcc gtctggcagg gcttccgcga gctgatggaa aaggccctca ccgtgtggga
    38401 ctggggcgag gccatcgtgg tcctcaacct gatcgtcaag ccggcggtcg aggagacggt
    38461 gctgcgccgc ctgggagaat ccgcccgcca caacggcgac accctgttgg gcctgctcac
    38521 cgacgcccag ctcatcgacg tggcgcgcca ccgccgctgg gctgccgcct tcgtggccat
    38581 ggccctggag acgccgggca accgggaact catcgccggc tggatcgccc agtgggaacc
    38641 gctggccgac cgggccatcg acgcctactg cgcggccctg cccgacgtgc ccgaagccgc
    38701 cgccgcggcc cgtgccgcca cccgcgacct gcgtcgcagc ctgggcttct gagcccgccg
    38761 ccacgggcga ccgctgcggt cctgtcgggc cgcggcggtg cgccgggaga cgccatgaat
    38821 ccacccctca tcgtcaacga gcgggacggc agccgtttcg cgcaggcccc tggcgacacc
    38881 gttctgcgcg ccgccctgcg ggccggcatc ggcctgtcct acgaatgcaa ttccggcggc
    38941 tgcggcggct gcaagatcga gctgctgcag ggcgacgtgg agactctgtg gcctgacgcg
    39001 cccggcctgg gcgagcggga tcgccgccgc ggccgtcacc tggcctgtca gtgccgcgct
    39061 gtcggcgaca tcaccatcaa ggcccccacc gcgcccgagt acgtgccgcc gatcctgccc
    39121 cggcgccgtc gcgccttcct gatcggcacc accgacctga cccacgacct gcgcgaattc
    39181 cgctttgccg ccgacggtcc ggccgacttc ctgcccggcc agtacgccat gctggatct
//
LOCUS       DAOQMM010000021        38796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_262_length_38796_cov_51.165871, whole genome shotgun sequence.
ACCESSION   DAOQMM010000021 DAOQMM010000000
VERSION     DAOQMM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 38796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38796
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_38796_cov_51.165871"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLH85_05590"
     CDS             complement(<1..1246)
                     /locus_tag="PLH85_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB64065.1"
                     /translation="MANDSIPTFPQPDLAAWNKAAAKSAPEGDVAKLNWVTPEGITVK
                     PLYTAADVNGLSFTDTLPGFEPFLRGPQATMYAVKPWTIRQYAGFSTAEASNAFYRKA
                     LAAGGQGVSVAFDLATHRGYDSDNPRVVGDVGKAGVAIDSVEDMKILFNGIPLDKISV
                     SMTMNGAVLPILAGYIVAAEEQGVSQDKLSGTIQNDILKEFMVRNTYIYPPTPSMKII
                     ADIFQYTSSHMPKFNSISISGYHIQEAGANQAIELAFTLADGLEYVRTGIKSGMDVDT
                     FAGRLSFFWAVGMNFYLEIAKMRAARLLWWRIMKQFNPKNAKSMMLRTHSQTSGWSLT
                     EQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARVARNTQIIIQEETH
                     ICNVVDPWAGSYMMEKLTQDMAD"
     gene            1523..2197
                     /locus_tag="PLH85_05595"
     CDS             1523..2197
                     /locus_tag="PLH85_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB64066.1"
                     /translation="MNATRIAPLALYQEVAERLRQRIFAHELPPGTWVDEQALAAQYG
                     ISRTPLREALKVLASEGLVTLKPRRGCYVTEISERDLDEIFSVMALLEGQCAASTARR
                     AGDGDLVRLHTIHDELEKAAASNDIDGFFEANQAFHQELQRIANNRWLTQAIVDLRKV
                     IKLSRHHSLFIDGRLQQSLLEHRVILAALDRRDEAGAEAAMREHISSARRALARIAST
                     RDEAAA"
     gene            complement(2297..3961)
                     /locus_tag="PLH85_05600"
     CDS             complement(2297..3961)
                     /locus_tag="PLH85_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB64067.1"
                     /translation="MIARLNLALLGLLAPALACAGDAISATEKQPLNLHAIGMFVLFV
                     SMTLGITYWAAGRTKSAADFYTAGGGITGFQNGLAIAGDYMSAATLLGLTALVYTKGV
                     DGYIYMIAFFAGWPIILFLMAERLRNLGKFTFADITSYRLNQSKVRTMAAVSSLTVVC
                     FYLVAQMVGAGQLIKLLFGLDYTIALFVVGALMMVYVTFGGMVATTWVQIIKACMLLA
                     GGTLVMVLAMSQFGFSFENLMTQAVSLHSLGEKILFPGSLLPDPVTALSLGLGLMFGT
                     AGLPHILMRFFTVTNAKEARKSVLYASGFVAYFFNVILLMGFSAIIIVGQNPDFFEGG
                     KIGGKIIGGGNMVAMHLAKAVGGNLLLGFLSAVAFATILAVVSGLALAGAAAISHDIY
                     ARVIRGGKATEMEELRVSKLSSVALGIVAILLGIVFEKMNVAFMVALAFGIAASANFP
                     VLILSMYWRGLTTRGAVVGGYVGLVAAVLFVLLSKSVWVTVLGNAQAIFPYDQPALFS
                     MPLAFLVTIVLSKLDNSAEAQAERAAFEDQYVRAQTGFGAAEASQH"
     gene            complement(3961..4293)
                     /locus_tag="PLH85_05605"
     CDS             complement(3961..4293)
                     /locus_tag="PLH85_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNB64068.1"
                     /translation="MSTASQSAVYARIRSNPRFAELVRKRQGFATILSVIVLTIFYGF
                     FMVVAFNPQLIGQRLSEGSYVTVGIAAELFMFIFFWILTAVYVKRANSEFDDITTAIV
                     NDAAKEVK"
     gene            4532..5314
                     /locus_tag="PLH85_05610"
     CDS             4532..5314
                     /locus_tag="PLH85_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB64069.1"
                     /translation="MSGEIVLQRDGGIATLSLSNPDKLNAVDAGMWRALHATQTALNA
                     DPDLRCVLLRGAGDKAFAAGGDIEEFLTVRATVDDALHYHEDLVAAALDAIRACPVPT
                     VAMIQGACIGGGLEIAGCCDLRIAGESSRFGAPINKLGFSMYPGEMAGLLDLVGPAVL
                     LEILLEGRILSAREALAKGLLTRVVDDEEVEREALATVSRILKGAPLVARWHKQWVHR
                     LADRRPLGTDEKRAAFDFLATEDYREGLDAFFAKRTPDFKGR"
     gene            complement(5330..5998)
                     /locus_tag="PLH85_05615"
     CDS             complement(5330..5998)
                     /locus_tag="PLH85_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64070.1"
                     /translation="MSDATVRLQLHFELELATPPELAGLDHAALCRTLADALGPTVLR
                     GLPAIAGKQLTPLGARVVRHHHHLDAAAQAARRVERAQVIQVAPHLTDAEVDTLAQRA
                     AARLPAAESEQAAYLRGQALALVNEYRLVPCAVEAQLTSGKPAHIEGRLNLTNGHIFL
                     DASHRQTRLQAGQGALRVSIAGTPVALAAECSGHTLTGPVLDVAVASLVPHRDVLLAR
                     WQAG"
     gene            complement(6083..6889)
                     /locus_tag="PLH85_05620"
     CDS             complement(6083..6889)
                     /locus_tag="PLH85_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB64071.1"
                     /translation="MELHWLAAYLLLGAFVGFFAGLLGVGGGAIMVPMLTTLFAAQGF
                     PREHMVHLALGTSMAAIVVTSISSLRAHHAHGAVRWEIVKGIAPGVLLGTFGGSFVAS
                     RAPTAPLALFFASFTAYVAVQMILNKKPKPSRELPGPAGLAAVGGGIGFISSLVAIGG
                     GSLSVPFMTWCNVKMQHAIGTSAAIGLPIAVAGALGYLINGWGTEGLPAWTAGFVYLP
                     ALVLVSAVSSLTAPIGARLTHRLPVATLKKVFAGVLILLSAKMLHTVFGG"
     gene            complement(6889..7371)
                     /locus_tag="PLH85_05625"
     CDS             complement(6889..7371)
                     /locus_tag="PLH85_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNB64072.1"
                     /translation="MRFWLMKSEPDDVSIDDLAARPDQIVPWYGVRNYQARNFMRDAM
                     TVGDGVLFYHSGCAEPGIAGIARVASAAYPDATQFDPASRYFDPKATPEQPRWVHVDV
                     AFVRKTRLLGLSELRTHPELATLRVLARGNRLSITPVEPAEWRFIVGDLLGATDLKDG
                     "
     gene            7486..7719
                     /locus_tag="PLH85_05630"
     CDS             7486..7719
                     /locus_tag="PLH85_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64073.1"
                     /translation="MSIPTERRKNLHLRDLVDKAYALIEPFFDPANAWNGQTLEHLAY
                     RVVRENLPGIPPEDVQVVVSAATRIYRAKIGPQ"
     gene            7778..8635
                     /locus_tag="PLH85_05635"
     CDS             7778..8635
                     /locus_tag="PLH85_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB64074.1"
                     /translation="MTRLYPLLFVLLWSTGFIGAKFGLPYAEPLVFLCIRYTLVIALM
                     GAAALLARAPWPQDPRQWLHIGLTGVLVHGVYLGGVFTAIRHGLPAGVTALVVGLQPL
                     LTALGAGVLLGERVLPRQWAGLVLGFAGVGLVVAHKVAAAADAHGLTAMLVPALAALA
                     GITVGTLYQKRFCPAFDLRTGAVIQFVPSLALSALVAWHTESLTVAWTGSFVFALMWL
                     VLVLSVGAISLLNLLIRSGSAVNVASLFYLTPPTTALIAWAAFGETLTGLALAGMGLT
                     AFGVWLARR"
     gene            8632..9180
                     /gene="ruvC"
                     /locus_tag="PLH85_05640"
     CDS             8632..9180
                     /gene="ruvC"
                     /locus_tag="PLH85_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNB64075.1"
                     /translation="MSLTTERVATRILGIDPGLRITGFGVIDTLGSELRYVASGCIRS
                     GEGSLPQRLKVILDGVREVIATYRPDQAAVEIVFVNVNPQSTLMLGQARGAAICGAVS
                     CELPVAEYTALQVKQAVVGHGKAAKQQVSHMVERLLTLEASPSPDAADALACAICHAH
                     ASAGLAALAGVGPRKRGGRLTR"
     gene            9305..11140
                     /locus_tag="PLH85_05645"
     CDS             9305..11140
                     /locus_tag="PLH85_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNB64076.1"
                     /translation="MSAPAALPPDALREHRLSLAEVTDLLVADRLVDRPDADDFLNGP
                     ARLRGEIHPLVLLAEQQWTARGAPSRPLDLETLTEWLAGRVSLDYFRIDPLKIDFTAV
                     TAVMSAAYAARFAILPVKVTPQEVVIATAQPFLREWEGELAGILKKRIHRVLANPLDI
                     ARYLVEFHNLARSVKQAAQGNHGGGVSSFEQLVELGQTNRQYDANDQHIVNIVDWLWQ
                     YAFEQRASDIHMEPRRDMGLVRFRIDGVLHQVYQMPMPVMNAMTSRIKILGRMDVVEK
                     RRPQDGRIKTRVASGKEVELRLSTLPTAFGEKLVMRIFDPQVLVRTLAELGFSDTDEG
                     RWHTITRRPHGIVLVTGPTGSGKTTTLYSTLKQLATPEVNVCTIEDPIEMVEPSFNQM
                     QVQHSIDLGFAEGVRALMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDA
                     PSAVTRMLDLGVPAYLLSATLLGIMAQRLVRILCPHCKTAAPLTPDEEAQWDHLVTPW
                     KAARPSRLHHPVGCMECRMTGYLGRIGLYEILLLSPELRRIVVGSGDLAQVRLQAIRE
                     GMKPLRISGALKVAAGLTTLEEVLKVAPPAEDGHERMPEDGSGRP"
     gene            11560..12567
                     /locus_tag="PLH85_05650"
     CDS             11560..12567
                     /locus_tag="PLH85_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNB64077.1"
                     /translation="MSVRRLALALSLCGATAAIAASPARPQEPITPLAPTKVSAPALV
                     ELGKKLFFDPRLSRSGFISCNSCHNLAMGGTDNLKTSIGHNWQEGPINAPTVLNSSLN
                     LAQFWDGRAPDLKAQAGGPIANPGEMASTHVLAVEVIQSIPGYSAEFKQVFGSDKVDI
                     DKITRAIAAFEETLVTPNSRFDQWLKGNKKALNADEVAGYTLFKDSGCVACHNGPAVG
                     GNSFQKMGVVAPYTASSPAEGRSAVTGKDADRFNFKVPTLRNVELTYPYFHDGAATTL
                     KDAVDTMGRIQLGKHFTDAENARIVAFLKTLTGAQPNFKLPILPPSSDATPRPEPFGR
                     K"
     gene            12717..14000
                     /locus_tag="PLH85_05655"
     CDS             12717..14000
                     /locus_tag="PLH85_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNB64078.1"
                     /translation="MPHTASPQDVFVTPEQLCVGLFIHVDLPWFSHPFSFNSFKIKSD
                     EQIATLRTLGVSRFRCDPARSDLPPAVGASEPRDAPAPPPEAPAADDPALAAKRERIL
                     RLQQRKRQVIEVEQAFVKAANVMRDIGKNLFARPKECLEEVDELVNQMVVAFLDQPEV
                     ALHVIGEHAGGEEAYYHGLNCSILAMMLAGELGLPRAAGQTLGVGALLHDIGLVDIPD
                     RIARARHELTPPERHLREMHCDYGVRLARQIGLPEPVLRIIAQHHEMADGSGYPKALK
                     GDQIDPCARLVALVNYYDNLCNPGDLAKALTPHEALSLMFAQRRGRFDAQALQVMIRC
                     LGVYPPGTVVKLSNDALALVASVNPEKPLRPWVTVYDPDIPKDEAIMLDLEQEPDINI
                     AKALRPIVLPPEVYEYLSPRKRVTYYFDANRRRGQ"
     gene            13997..15895
                     /locus_tag="PLH85_05660"
     CDS             13997..15895
                     /locus_tag="PLH85_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64079.1"
                     /translation="MSAAHDAILLAGAEELLMLVDPATLAILAVNPAAERLLGLRAEA
                     LVGQPVTEIECALADLFFWEEVRSGGGADEQVMEGLYRRADGTLLSARKCVRRVPAGM
                     LVRATDIGAQKKAETELALMTAQLRATLEATADGILVIDADGRVVNMNRRLARQWQLP
                     ETLLAHRDDDALIAHMAAALESPAPFRSLLTDHLFDLTDTFDTFVFRDGRRCEITSRP
                     ARQAEQVIGRVFCFTDVTERHRSQQALIAARDAADAANRAKSDFLAIMSHEIRTPMNG
                     IIGMAQLLGMSEHRPEEREYIDTILTSSDALMGIINDILDYSKIEARKLTLERISFDL
                     HQLVTDVERMFRPKALEKGLQFGATLATTLPRVVMGDPTRLRQILVNLVSNAFKFTPQ
                     GAIRIDVEPAEALRGGRVIVRFAVTDTGIGIPADKCEHIFTPFEQADMSTTRRYGGTG
                     LGLAICRMLCTLMEGEIRVDSQVGAGSRFDFSVALDVGVAEERPTVEPPRALLRRDTR
                     ILIAEDNPVNQVVLARLLSRLGATQPAFARDGREAVDLGTADRYDLIFMDARMPVMSG
                     IEATQALRERGCRAYIVGCSADTLPDDIRLALDAGMDDYLTKPVSHEALGAAIERWRR
                     HLGETPTG"
     gene            16099..16389
                     /locus_tag="PLH85_05665"
     CDS             16099..16389
                     /locus_tag="PLH85_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64080.1"
                     /translation="MKRHPSLAAALLLTLGCAATVPAYAHGGGEGHHHGAWGWGPRHH
                     HYYPVREPVMVVPPQAYYAPPPVYYAAPPRPVYYTPQPSVVIGIPPLVIPLR"
     gene            complement(16373..16972)
                     /locus_tag="PLH85_05670"
     CDS             complement(16373..16972)
                     /locus_tag="PLH85_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNB64081.1"
                     /translation="MNFLAHAWLAGPHPADRLGGLMGDFIKGPLPGALAPAVASGVRL
                     HRRIDVFADSHPAFLRSRARVSTARRRVAGVMVDMFYDHFLARDWAEFHPEPLADFTA
                     AQYRLLEEAPGLPERLQKILPSMRDDDWLASYRDVETVAYALERMAGRLRPGNPLAGG
                     GAELLMHYAEFAADCREFLPDARAFAQAFWQRDGFSAAE"
     gene            17209..17583
                     /locus_tag="PLH85_05675"
     CDS             17209..17583
                     /locus_tag="PLH85_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64082.1"
                     /translation="MTAAPVILLVDDSRVSRMLCGAIVRERRPDAILVEAGNGDEALS
                     CIATLVPHLAVLDVNMPGMSGLELAERIRNDHPDMRIALLTANVQDSVRQRAAALNAA
                     FFAKPINDTVVGRILDLLESHP"
     gene            17580..18209
                     /locus_tag="PLH85_05680"
     CDS             17580..18209
                     /locus_tag="PLH85_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNB64083.1"
                     /translation="MRPLSELECDALSEAFNLALGEAAATFASIVREEITLSAPVVEI
                     ITRDTLIARLQAAGCGNGTSRICSIDQRFHADMHFDSDALLLFPEQGSLEIVRRMIGD
                     ETPIEQITELEQDALAEIGNIIINSCMSSLANLFGTEMHGSLPVVRSCTADTLLGVRQ
                     ASDVILVAQINMRMSAHDISGYVLFIMDVPSIESFMRQVARLFNLPERV"
     gene            18217..19560
                     /locus_tag="PLH85_05685"
     CDS             18217..19560
                     /locus_tag="PLH85_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB64084.1"
                     /translation="MSSTPALLDALDVGIILVDRDGRVQHWNRWMVTFSGLPARDILG
                     SRLSTAFGTLMDPRIAVAIREAIDFGRSSHLSQVFHPAPLPLHNPADPGAPRLRQMID
                     ASPVDDGDGRSCLLQVRDVTETVRREALLRSQARRLQEDLARLSAAQAELQRSELRFR
                     ELARQAPAGLFETDAQGRLIFLNERCAQMLGRSPADLPDEPWTHLLPPDDVDRMTARW
                     RRAVQTGNRFSEQFRYRHPNGSDLWIRAEAGVVREGSGIGGFIGTMVDVTEFHEQVVR
                     NEFRANHDSLTRLLNRERFDVLVRAAVSGAREVGQKIAILFIDLDQFKAVNDTHGHAA
                     GDRVLKSVAGRVRRVVRGEDVVARYGGDEFAILLHDAGDEAMLKRLTANIERSIALPI
                     PVGTGQVQIGCSIGVAIYPDHGRDPLTLLAHADRQMYRIKRSRWEAAEPDRDPVA"
     gene            complement(19582..21726)
                     /locus_tag="PLH85_05690"
     CDS             complement(19582..21726)
                     /locus_tag="PLH85_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNB64085.1"
                     /translation="MGFKPLKLLAFLLGGLSAVMGAGALYLYATFDGARLAAELTQSL
                     RQQTQRTVRLDGPVALSLFPRLSLRLPATTVSDPRSDGAFLAFERAELRVGLWPLLSR
                     RVAIEAVELQGPQLTLRRRRDGAVNTDDLAALLQGQEGDTALPLTADVHRLVVHDGRV
                     DWLDEAGGRRLGVTGLTLETDDLASQSVGRLSLTGHLRGDSPNVEVDLRVASDYRLDA
                     VGGRHALRHLSLSAVGVLAGRGDLEARASLDELAWVTGGELVVKGAAGDLRARAAEGA
                     PVLHLATPDLRLTANGPVAATVDADARWGSEAEGGRLTARLSDLQARAAGISVTADLR
                     TGDDRLNLQGRGAGDWRAAESLAQLRGVVLEGTWTRRGGASSPVRAGADLQVDTARGS
                     AGGTVDLRIDGSRINGTWALSRFAPWAGRFELEADRLDADRLFAATGDRWDLSGLHGF
                     DVDGQLRIGSLRAAGLQFERVRLPLSLHGGRLVSRGHAAGLYGGAVAGNFTLVADGNK
                     AFYTGQINGVSVEPLLRDATGREALSGTLNAFVDVATAAGRRSALAGALAGTGRFVLG
                     HGAVRGIDPFAALREWQAAIAARRGARRTFRDAEQAGLTEWSGSFGIERGVVSSADLQ
                     ARSAQWRASGHGVLDLAAGRIDVQQRLTVLALPPGAEGIALASLRGATLPVRVTGPLG
                     RAEWLLEPSSGVQSAPPGGPGAGRGPADGGRR"
     gene            complement(21767..24097)
                     /locus_tag="PLH85_05695"
     CDS             complement(21767..24097)
                     /locus_tag="PLH85_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 and HATPase_c domain-containing protein"
                     /protein_id="HNB64086.1"
                     /translation="MKSGTGTVLRRPTLEWLAVTTLCIVLAVFAALEGWLWRIDQTIY
                     DSFLSSFRRATSANVAIVAIDDASLAELGSWPWSRRVHAALLDRLHDAGARAVALDLI
                     LDTPSRDDPAADTVLAQSMRAFGRVALPLTQATTGVLLTGEILPLPEFVAAAAALGHG
                     QVEFDPDGIARSVYLWEGFGSARHPQLALALLRIVDPEVAARYAQPGPGDDDDRLQWV
                     RATWLRVPFAGPPGTYPYYSYAAVLRGEVPADALRDKVLLVGATASGMAEAVPTPMSG
                     LSRPMPGVEVDANVYDALRRGDAVRPLSAGTSAGVAALAVLVAMLALFYGDPRTGLLV
                     SVLAGLAALALAAVALQAGSMWFGPAPAVLGCMLAYPLWSWRRLEWAQRYLDAELAAL
                     EREGDALPSEVDRGAADRLERRIRIVRHVARQQRDVRRFIADTLEHLPIGVVVIDPGN
                     RVVLHNERARHLLQAFRGEDLLAALRSLPWPVYLPMRDGLPDLAGFAGIRQFELPVGR
                     RHLLLSVAELVAGTGSLSGRVIGLADVTVMHEAQRTREDTMHFLSHDLRAPLASILTL
                     IDGYKGDPAAQAEQIPRIARYARSALDLADGLFRLVRAEGSDPKDFVELDLAGLVDSA
                     ADEVWPQAHARGIRVAVDTRAAETEEPIVRGDFSLLRRAVINLLTNAIKYGSPDSTVS
                     AELAAEGADWVIRVRGQGAGIPPDALPRLFKRFSRIVEEGRDRPSGVGLGLLIVKTVA
                     ERHGGNVSVESTPGVGSMFEIRLPLRMRPDAPSRPD"
     gene            complement(24094..25683)
                     /locus_tag="PLH85_05700"
     CDS             complement(24094..25683)
                     /locus_tag="PLH85_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNB64087.1"
                     /translation="MALHRHRILSAVLGVLAWSGGAFAADEDIVYTARPGDTLIGLQK
                     QLLAAPFGWKGVMLHNRIAEPRRIPTGTQVRIPEAWLRVEARSAQVVGVHGEATLNGR
                     PLALEDQVPPGAVLRTGNASFVTLRMPDESRLTLQPDSEARLEKIQGFAGLTGQTAQI
                     HLERGRIENTVAAQRGPAARYRIRTPSASVSVRGTEFRVGSDGTDGRARAEVTGGEVR
                     VGAETGPATAVAMGFGVTAEPGGGVQPPQALLPAPALEGLATVHERTAVEITLRPVDK
                     AVAYRAQLARDAAFTDVVTEAVSPAPPVRLENVPDGRLWVRVRAIDAAGLEGFDTQRA
                     LEVRARPVPPDAAGPLAGAAFVGGPVHFEWTPVADAPGYRFQVLRRAAPEVLLIDREV
                     VTPGIDLTLPRGEFRWRVATLKPAGGQGPWGDARALAVTRPIGVPTVRRYNDRLRFEW
                     SGDDGQIYDFQLARDAGFGDLVVNQRLGEAAITVPVPAGGRYFVRVRGTDAQGAASPY
                     TPVQVLYRLPLLMWTLSNAAP"
     gene            25903..26322
                     /gene="mscL"
                     /locus_tag="PLH85_05705"
     CDS             25903..26322
                     /gene="mscL"
                     /locus_tag="PLH85_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNB64088.1"
                     /translation="MSIIQEFKEFALKGNVVDLAVGVIIGGAFQKIVDSLVKDLLMPV
                     LGKLLGGVDFKHLYINLGDKTYETLDAAEKAGAPLFKYGVFINTVIDFVIVAAAIFVA
                     IKAMNKLKREEPVPAPEAPVEAEDVKLLREIRDALKK"
     gene            complement(26372..27115)
                     /locus_tag="PLH85_05710"
     CDS             complement(26372..27115)
                     /locus_tag="PLH85_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB64089.1"
                     /translation="MRFAILEDDPAQMEVLTGWLREAGHDVHGFAMARELMRVASRES
                     FDLFLVDWVLPDLTGEQVLRWLRTERNNDTPAIFITSRDAEDDIASAFSAGADDYLVK
                     PPRRIELLSRIEAVLRRTQPRQVSQLLQVAPYSFDLIRKMVTVNGEPVEMTDKEFELA
                     AFLFRNMGRLLSRGHLLEAVWGRNPDLATRTVDTHISRVRSKLNLRPENGFRLTPTYN
                     FGYRLERVDAANLAPDGAPAEGADLSSQV"
     gene            complement(27193..27834)
                     /locus_tag="PLH85_05715"
     CDS             complement(27193..27834)
                     /locus_tag="PLH85_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB64090.1"
                     /translation="MLQYKIVPVTPFEQNCSILWCDASRQAAVVDPGGNVDRILAAVQ
                     ALGVTVERILLTHGHIDHAGGTADLARQLAVPIEGPDREDAFWIEGLPQQSRMFGFPP
                     VGAFTPDRWLADGDTVQVGEETLDVIHCPGHTPGHVVFFSPSARLAVVGDVLFAGSIG
                     RTDFPRGNHAALIASIRTRLWPLGKDVAFIPGHGPMSTFGDERDANPYVGDYA"
     gene            complement(28223..28858)
                     /locus_tag="PLH85_05720"
     CDS             complement(28223..28858)
                     /locus_tag="PLH85_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64091.1"
                     /translation="MMRRLLFCLLAAVSVPAGAQSSAKAPRVFCCPDASGHQICGDVL
                     PEQCYGKSYREMSPQGGVRRLVEAPLTPEQLARREAEERARKAEQARQRAETRRNQSL
                     LETYSSVQDIDNRRDKAIESVQSELKQAEARYVQLQARRQGLTREAEFYQKRSMPVAL
                     ANSLRESDSELAAQWSVIEVKRRDIENIRARYEQDRARYIALTDPRAPDIR"
     gene            complement(28865..29512)
                     /gene="pyrE"
                     /locus_tag="PLH85_05725"
     CDS             complement(28865..29512)
                     /gene="pyrE"
                     /locus_tag="PLH85_05725"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNB64092.1"
                     /translation="MDFSRDFIQLACDKGVLRFGEFVTKAGRLSPYFFNAGLFNDGES
                     FGRLCDFYARTLIAAHLPCDMLFGPAYKGIPLVAGTAMRMAERGVNLPFCFNRKEAKD
                     HGEGGTLVGAPLAGRVAILDDVISAGTSVRESVEIIRAHGATPAAVVIALDRMERGTG
                     SLSAVQEVQRTYDIPVLAVANLADLIAYLKDSPALAENLGAVTRYRETYGVAREA"
     gene            29551..30339
                     /locus_tag="PLH85_05730"
     CDS             29551..30339
                     /locus_tag="PLH85_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB64093.1"
                     /translation="MLRVVTANLNGIRSANVKGFLPWLALQQADVVCLQELKAQSADL
                     PPETVTPNGFRGFFSFAEKKGYSGVGIYAKKIPDQVIYGFGIPEFDAEGRYIQADFGN
                     VSIISLYLPSGSSSDERQAAKFRFMAHFLPHLAQLRCSGREVIVCGDWNIAHQAIDLK
                     NWRSNQKNSGFLPEERAWLSRLFDEQGWVDTYRRLHPDATDACYTWWSNRGQAWAKNV
                     GWRLDYQIATPGIAAQVRRCAIYKEQRFSDHAPLTVDYDHPFGD"
     gene            30467..30952
                     /locus_tag="PLH85_05735"
     CDS             30467..30952
                     /locus_tag="PLH85_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNB64094.1"
                     /translation="MANPFHDPASATVQNLEAAFAGESMAHIKYRYFAKLCREMGDEE
                     TARAFEATADQEVMHAFGHLDLLYPKAKMSPARALEFAIEGETYEYTEMYPAFRHTAV
                     AEGHSAAVQEIDEQIAESREHAEQFKAVLEKAAKRFAALAKVEERHAAHYQARLDQID
                     A"
     gene            30987..31151
                     /locus_tag="PLH85_05740"
     CDS             30987..31151
                     /locus_tag="PLH85_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015389172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNB64095.1"
                     /translation="MKTWQCIVCGFIYDETAGLPEEGIAPGTAWADIPDDWACPDCGV
                     AKSDFEMIEL"
     gene            31203..32357
                     /locus_tag="PLH85_05745"
     CDS             31203..32357
                     /locus_tag="PLH85_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB64096.1"
                     /translation="MTAPLLILGTGLAGYTLAREVRKLDRDRPLVLVSRDDAPFYSKP
                     MLSNALAAGKSATTLVMKSAERMAEELGATILPRRRVASLDPAARHVQFDGGETLAYG
                     DLVLALGADPIRLPLAGDGADDVLSVNDLDDFARFSARLDGVQRVVILGAGLIGCEFA
                     NDLLARGISPSVFDVAPWPLGRLLPDAAGRHFQARLEAAGVRFHLGQAAQAVHRDGPG
                     YRVELGDGRQEPADLVLSAVGLRPRTALAAAAGLTVGRGITVDRRLATSDPHIFALGD
                     CAEVDGLHLPFVLPLMQQARALARSLTGTPTDVSYPPMPVLVKTPAIPTIVAPPPIGA
                     AGVWRIVEDTPDAGLEARFEAPDGSLRGFALLGPATARKQTLSAALPALL"
     gene            32441..34090
                     /locus_tag="PLH85_05750"
     CDS             32441..34090
                     /locus_tag="PLH85_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB64097.1"
                     /translation="MVPPFTRPVLRLLILWAGLLTALAALPAFAAPASSHPQKVQSAP
                     PDLPPAADSAPVLLGGEEVFRIAAPFKAYSPAVRARQISRRLADLATRGTAPEIRVEE
                     SDISSDLLADDQVLLSVFDADAAAEGIPRADLAARHAATLGAALARYHDEHRHEALLI
                     GLGKSAGAVLAALALLTLFERGYRRLREAILRKADETIASVERRSFSLVRGSYLRTPL
                     LALLHGIRLAVWASLLYAALHLSLASFPQTRRFATHLSELVLTPLGTLGHGLLDAIPG
                     LLVVVIIGVLARWLIRSASFIFERIGDGRIVLDGFYPEWAAPTRRIVHLLLIAGAVMI
                     AYPYIPGSDSAAFKGVSIFLGVLLSLGSSGVISNIVTGFMITYMRAFKVGDVVRIGET
                     TGIVVSSSLLVTRLRTIRNIEITVPNSQILNGQVTNFSVTGNPTVSTQVTIGYDTPWR
                     QVHALLLDAAARTTGIVHQPPPFVLQTALEDFYIRYELMCVVDNPTRMPFILSALHQA
                     ILDAFNEYGVQIMSPHFIDNPGNPAVVPREQWHAAPARPPA"
     gene            34252..34560
                     /locus_tag="PLH85_05755"
     CDS             34252..34560
                     /locus_tag="PLH85_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNB64098.1"
                     /translation="MSETQVTKDKLVADFKAVVADTEELLKLTAGTAGDKVADVRARL
                     TDKLGTAKEKLQDLEAAVVAKTKAAARATDDYVHDNPWKAVGVAAGVGFLLGLLVNRR
                     "
     gene            34569..34952
                     /locus_tag="PLH85_05760"
     CDS             34569..34952
                     /locus_tag="PLH85_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNB64099.1"
                     /translation="MSDSAPPRLGESLRGLADTGLGVLQTRLELFAVELKEEKLRVGS
                     FLFDTVLAALFIGFGIVFLLAFLTVLMWDEHRLLALGLATAGLFAAGIYAATRAATRL
                     RAGSRLFAASLAELAQDRDALRPED"
     gene            34961..35245
                     /locus_tag="PLH85_05765"
     CDS             34961..35245
                     /locus_tag="PLH85_05765"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HNB64100.1"
                     /translation="MSPELVELALRKQRLQIRSAAQRQALAGHLAAFAPLLRGADAVT
                     DGLHWARRHPALLAGAAAFLLVTRPRRAVRWLRRGWFGWQLVRRARALVS"
     gene            35257..35814
                     /locus_tag="PLH85_05770"
     CDS             35257..35814
                     /locus_tag="PLH85_05770"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNB64101.1"
                     /translation="MTLDLDDIRRVRAEADCLCTPAEVDAALDRLAAAITERFHDRNP
                     LLYVVMNGGLVLAGRLLHRLDFPLEVAYLHATRYGHALQGTLLDWRVRPTQDLRDRHV
                     LVLDDILDEGHTLAAIIDYLKSEGARDVQSAVLVHKLHDRKAYPGMRADFTGLDVADR
                     FLFGCGMDYKGYWRNAAGIYALKGH"
     gene            complement(35856..37478)
                     /locus_tag="PLH85_05775"
     CDS             complement(35856..37478)
                     /locus_tag="PLH85_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB64102.1"
                     /translation="MNLNMQKIWVRLVIVIASLMALGSAGVLWWVAREQQHTAIEQAE
                     EFAQSVHQMTMAQLMFAKATRNYKRQGYYLEQVEQSRGLRHLKVLRGAATVQQFGERE
                     QGVIPADDPLEKAVLADGKARYEIREEEGQPVLKAVLPAIAEKRYLGKECLECHDEAK
                     EGDVLGAVSMSVQLDAVKRNLRESLLTTVAVALLLGVVLVAAVYVFVRNALARPLNGM
                     TRNLADIAGGEGDLTRRLKARRQDEVGLASAAFNRMMDSLQRLIGQVIRSSGDVAGAA
                     SALERGTARIADGSTEQARRSASAAAGMEEIVGSIAQVADLSTRVQALATHSMTCTHH
                     GNETLTELDDKLHGIDRAVQEIVGTVENFVNRAESITAMTQQVKGISDQTNLLALNAA
                     IEAARAGESGRGFAVVADEVRKLAEQSHRSAAAIDTITGALGEDSSAARTAIQHGLEV
                     LRSSRESMRRVAEALGEANRAVEDAVRGTAEISAATGVQRDVSGRVAADVEGIAQLAE
                     RNGAEIADAAVAARQLAGLAAELQAETGRFRV"
     gene            37674..>38796
                     /locus_tag="PLH85_05780"
     CDS             37674..>38796
                     /locus_tag="PLH85_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64103.1"
                     /translation="MTDAPRSPRSLRLFLSSPGDVADERQAVRRVLEDLALTPLLRTR
                     VRFDLVAWDDPAAPVPLDATETPQLSVNRYKGLPRDCDLTVVILWSRLGTPLPSHVTR
                     PDGSAYASGTVWELEDARTGRKPLWLYQRTATPQVDLDDPEFDAKRAQYHAVKAFIAD
                     FRAPDGSLSAGINTHADPAAFTILFRQHLEAHLGGLLFDAPPVAPDSPRIDHILLALD
                     AQLAAKDSTIAERDGEIARLKAELDALRRSAIADTLTRAAASDATPAEQAAAQALEAG
                     DSAPAEALLRADETALTPADRPPPPPNAAPPRTSPASRAPSSSATTPRAPSPPTSARP
                     NTIRKTSGRGSTSATSTSPWAISRRPRRPTRAPKPTSPTA"
BASE COUNT         6413 a        14505 c        12079 g         5799 t
ORIGIN      
        1 tgtcggccat gtcctgggtg agcttttcca tcatgtagga gccggcccag gggtcgacga
       61 cgttacagat gtgggtttct tcctggatga tgatctgggt gttgcgggcg acgcgggcag
      121 agaactcggt gggcagcgcg atggcttcgt cgagggcgtt ggtgtgcagg gactgggtgc
      181 cgccgaacac ggccgccatg gcttcgatgg tggtgcgcac cacgttgttg tacgggtcct
      241 gctcggtgag ggaccagccg gaggtctggc tgtgggtccg cagcatcatg gacttggcgt
      301 tcttgggatt gaactgcttc atgatgcgcc accacagcag gcgggcggcg cgcatcttgg
      361 cgatctcgag gtagaagttc atccccaccg cccagaagaa ggacaggcgg ccggcgaagg
      421 tgtccacgtc catgccggac ttgatgccgg tgcgcacgta ttcgaggccg tcggcgaggg
      481 tgaaggccag ttcgatggcc tggttggcgc ccgcttcctg gatgtgatag cccgagatcg
      541 agatgctgtt gaacttgggc atgtgcgacg acgtgtactg gaagatgtcg gcgatgatct
      601 tcatcgacgg cgtcggcggg tagatgtagg tgttgcggac catgaactcc ttgaggatgt
      661 cgttctggat ggtcccggac agcttgtcct ggctgacgcc ctgctcttcg gcggcgacga
      721 tgtagccggc gaggatcggc agcacggcgc cgttcatggt catggagacg gaaatcttgt
      781 cgagggggat gccgttgaag aggatcttca tgtcctcgac ggaatcgatg gccacgccgg
      841 ccttgcccac gtcacccacc acgcgggggt tgtcggagtc gtagccgcgg tgggtggcca
      901 ggtcgaaggc caccgacacg ccctgaccgc cggcggccag ggccttgcgg tagaaggcat
      961 tggaggcttc ggcggtggag aagccggcgt actggcggat ggtccacggc ttgaccgcgt
     1021 acatggtggc ctggggaccg cgcaggaagg gctcgaaacc gggcagggtg tcggtgaagg
     1081 acagaccgtt cacatcggcg gccgtgtaca acggcttgac ggtgatgcct tccggcgtca
     1141 cccagttgag cttggcgaca tccccttcgg gggccgactt ggcggcggcc ttgttccagg
     1201 cagccagatc gggttggggg aaggtcggaa tgctgtcgtt cgccatggcg gtctacccta
     1261 taaaattgtt cggccgctac gcgaccgcta ttgtgttgta cgccaggacc ggccgtgacc
     1321 gaacggtcac ggccgggctg ccgtgtcccg tgcagggccg ttttctccct ccctctgttt
     1381 ttcgcgccgc ggccggggcc ccgtcgccaa gggtatacaa cctgcacagg aacttgactt
     1441 gctcccttgg tgataatact ttatccataa ttatgaatgc aatagccgat ttgatccgtc
     1501 cgactcctac gcccgccgtc ccatgaacgc cacccgcatc gcccccctcg ccctctatca
     1561 ggaagtcgcc gagcgcctgc gtcagcggat ctttgcccac gagctgccgc ccggcacctg
     1621 ggtggacgaa caggccctcg ccgcccagta cggcatctcc cgcactcccc tgcgggaagc
     1681 gctcaaggtc ctcgcctccg agggcctggt gaccctcaag ccgcgccgcg gctgctacgt
     1741 caccgagatc tccgagcggg acctggacga gatcttctcg gtgatggccc tgctcgaggg
     1801 ccagtgtgcc gccagcaccg cgcggcgtgc cggcgacggc gatctggtcc gcctgcacac
     1861 catccacgac gaactcgaaa aggccgcggc gagcaacgac atcgacggct tcttcgaggc
     1921 caaccaggct tttcaccagg aactgcagcg catcgccaac aaccgctggc tgacccaggc
     1981 catcgtggat ctgcgcaagg tgatcaagct gtcccgccac cattccctgt tcatcgacgg
     2041 ccgcctgcag cagtccctgc tggagcatcg ggtgatcctc gccgccctcg accggcgcga
     2101 cgaggccggc gccgaggctg ccatgcgcga gcacatctcc agcgcccgcc gcgccctcgc
     2161 ccgcatcgcc tcgacccgcg acgaagccgc cgcctgacgc ggtcacggtc cattcaagca
     2221 aaaacccccg gtcgccgcga ggcgccgggg gtttttgctg ccaccgggtg cccgcaggca
     2281 cccggaacag gggcgatcag tgctggctgg cttccgccgc accaaagccg gtctgggccc
     2341 gcacgtactg gtcctcgaag gcagcgcgct cggcctgggc ctcggcgctg ttgtcgagct
     2401 tggacagcac gatggtgacc aggaaggcca ggggcatgga gaacagggcc ggctggtcgt
     2461 aggggaagat cgcctgggcg ttgcccagca cggttaccca cacggacttg gacagcagca
     2521 cgaagagcac cgccgccacc aggccgacgt agccgcccac caccgcaccg cgggtggtga
     2581 ggccgcgcca gtacatggag aggatcagca ccgggaagtt ggccgaggcc gcgatgccga
     2641 aggccagcgc caccatgaag gccacgttca tcttctcgaa gacgatgccc agcaggatcg
     2701 ccacgatgcc cagggcgacg gaagagagct tggagacgcg caactcttcc atctcggtgg
     2761 ccttgccgcc gcggatcaca cgggcgtaga tgtcgtggga gatggccgcc gcaccggcga
     2821 gggccagacc ggacaccacc gccaggatgg tggcgaaggc caccgccgac aggaagccca
     2881 gcagcaggtt gccgcccacc gccttggcca ggtgcatcgc caccatgttg ccgccgccga
     2941 tgatcttgcc gccgatcttg ccgccttcga agaagtccgg gttctggccg acgatgatga
     3001 tggcggagaa gcccatcagc aggatcacgt tgaagaagta ggcgacgaaa ccggacgcgt
     3061 agagcaccga cttgcgggct tccttggcgt tggtcacggt gaagaagcgc atcaggatgt
     3121 ggggcaggcc ggcagtgccg aacatcaggc cgaggcccag ggacagggca gtcaccggat
     3181 cgggcagcag ggagccgggg aacaggatct tctcacccag gctatgcagg gagacggcct
     3241 gggtcatcag gttctcgaag ctgaagccga actgggacat ggccagcacc atcaccaggg
     3301 tgccccccgc cagcagcatg caggccttga tgatctgcac ccaggtggtg gcgaccatgc
     3361 cgccgaaggt gacatagacc atcatcagcg cgcccaccac gaacagggcg atggtgtagt
     3421 ccaggccgaa gagcagcttg atgagctgac cggcacccac catctgggcc accaggtaga
     3481 agcacaccac ggtcagggac gacaccgccg ccatggtccg taccttgctc tggttgagcc
     3541 ggtaggaggt gatgtccgca aaggtgaact tgcccaggtt gcgcaggcgc tcggccatca
     3601 ggaacaggat gatgggccag ccggcgaaga aggcgatcat gtagatgtag ccatccacgc
     3661 ccttggtgta gaccagcgcg gtgagcccca gcagggtcgc ggcagacatg tagtcaccgg
     3721 cgatcgccag accgttctgg aagccggtga tgccgccgcc agcggtgtag aaatccgccg
     3781 ccgacttggt gcggcccgcc gcccagtagg tgatgcccag ggtcatggac acgaacagca
     3841 cgaacatccc gatggcgtgc agattgaggg gctgcttctc ggtggcggag atggcgtcgc
     3901 cggcacaggc cagcgcgggg gccagcaggc ccagcagggc caggttgaga cgggcgatca
     3961 tcacttcacc tccttggcgg cgtcgttcac gatggcagtg gtgatgtcgt cgaactcgct
     4021 gttggcgcgc ttgacgtaca cggcggtgag gatccagaag aagatgaaca tgaacagctc
     4081 ggccgcgatg ccgacggtca cgtaggaccc ttcggacaga cgctggccga tgagctgcgg
     4141 gttgaaggcc acgaccatga agaagccgta gaagatggtc agcacgatga ccgacaggat
     4201 ggtcgcgaag ccctggcgct tgcgcaccag ctcggcgaac cggggattgc tgcggatgcg
     4261 tgcgtagacc gcactctgag acgcagtgct catgattttt ccctccctcg agaaacttca
     4321 cttggatgtg gcgcgatctg cctgttggtc tgtgcctggg gcacaggggg caatcatttt
     4381 tatgtatagg gtcccttgcg ggcagggcga agccgcgccg acgcgacgcc caggctgaat
     4441 gttataacat atacaagatt ccataccaga acgtacgggc gtttttgccg gcggcgccgg
     4501 cctaccaccc caaaaaaaca cggaccccat catgagcgga gaaatcgtcc tgcagcggga
     4561 cggcggcatc gccaccctga gcctctccaa cccggacaag ctcaacgccg tcgacgccgg
     4621 catgtggcgc gccctgcatg ccacccagac cgccctcaac gccgacccgg acctgcgctg
     4681 cgtgctgctg cgcggcgccg gggacaaggc cttcgcggcc ggcggcgaca tcgaggaatt
     4741 cctcaccgtg cgcgccaccg tggacgacgc gctgcattac cacgaagacc tggtcgccgc
     4801 cgccctcgac gccatccgcg cctgcccggt gcccaccgtg gcgatgatcc agggcgcctg
     4861 catcggcggc ggactggaga tcgccggctg ctgcgacctg cgtatcgccg gcgaatcgag
     4921 ccgcttcggc gcgcccatca acaagctcgg tttctccatg tatcccggcg agatggccgg
     4981 cctgctggat ctggtcggcc ccgccgtact gctggaaatc ctcctggaag gccgcatcct
     5041 gagcgcccgc gaggccctcg ccaaaggcct gctgacccgc gtggtggacg acgaggaggt
     5101 ggagcgggaa gccctcgcca ccgtcagccg catcctcaag ggcgcgccgc tggtggcgcg
     5161 ctggcacaag cagtgggtgc atcgcctggc cgaccggcgc ccgctcggca cggacgaaaa
     5221 gcgcgccgcc ttcgactttc tcgccaccga ggattaccgg gaaggcctgg acgccttctt
     5281 cgccaaacgc acgccggatt tcaagggacg ctgacgcccc ggccggccct cagcccgcct
     5341 gccagcgggc gaggagcacg tcccggtgag gcaccaggct cgccaccgcc acgtccagca
     5401 ccggaccggt gagcgtatgg ccggagcatt ccgccgccag tgccaccggc gtcccggcga
     5461 tgctcacccg cagggcaccc tggcccgcct gcaggcgggt ctggcgatgg gacgcatcca
     5521 gaaagatgtg gccgttggtc aggttcagcc tgccttcgat gtgcgccggc ttgccgctgg
     5581 tgagttgcgc ctccaccgcg cagggcacca gccggtactc attgaccagt gccagcgcct
     5641 gcccgcgcag ataggccgcc tgttcggact cggccgccgg caggcgcgcc gccgcacgct
     5701 gggccagggt gtccacctcg gcgtccgtca ggtgtggtgc cacctgaatc acctgggcgc
     5761 gctcgacgcg acgcgccgcc tgggccgccg catccagatg atggtgatgg cgcaccacgc
     5821 gcgcccccag cggcgtgagc tgcttgccgg cgatcgccgg caggccgcgc agcaccgtcg
     5881 gccccagcgc atcagccagg gtccggcaca gggccgcatg gtcgagcccc gccagttcgg
     5941 gcggcgtggc gagctcgagc tcgaaatgca gttgcaggcg gacggtcgcg tcggacatgg
     6001 cgggcaccgg gtggcagtca tcagaaacgt ccacggcagc cccgtgtcat ccttgagcag
     6061 ccccccgcgc cgccgaacgc cctcagccgc caaacaccgt gtgcagcatc ttggccgaca
     6121 gcaggatcag cacgccggca aagaccttct tcagggtcgc caccggcagg cggtgggtga
     6181 ggcgggcgcc gatgggggcg gtgaggctgg acacggcgct caccagcacc agggcgggca
     6241 ggtagacgaa gcccgcggtc cacgccggca gaccctcggt gccccagccg ttgatcaggt
     6301 agcccagcgc accggccacg gcgatgggca ggccgatggc ggccgaggtg ccgatggcgt
     6361 gctgcatctt cacgttgcac caggtcatga agggcaccga cagggagccg ccgccgatgg
     6421 ccaccaggga cgagatgaag ccgatgccgc cgcccactgc ggccagcccc gccgggcccg
     6481 gcagctcgcg ggacggcttg ggtttcttgt tgaggatcat ctgcaccgcc acgtaggccg
     6541 tgaagctggc gaagaacagc gccagcggcg cagtgggcgc ccgcgaggcg acgaaggagc
     6601 cgccgaaggt gcccaacagg acgccggggg cgatgccctt gacgatctcc cagcgcaccg
     6661 cgccgtgggc gtggtgggcg cgcaggctgg agatcgaggt gacgacgatg gccgccatgg
     6721 aggtgcccag ggccaggtgc accatgtgct cccgcggaaa gccctgggcg gcaaagaggg
     6781 tggtgagcat gggcaccatg atggcgccgc cgcccacccc gagcagtccc gcgaagaatc
     6841 ccacaaacgc ccccagcagc aggtaggcgg ccagccagtg cagttccatc aaccgtcctt
     6901 cagatccgtt gcgccgagga gatccccgac gatgaagcgc cactcggcgg gttccaccgg
     6961 cgtgatcgac agccggttgc cgcgggcaag gacgcgtagg gtggcaagct ccgggtgtgt
     7021 gcgcaattcg gacaaaccca ggaggcgggt cttgcgcacg aaggccacgt ccacatgcac
     7081 ccagcggggc tgctccggcg tggccttggg gtcgaagtat cggctcgccg gatcgaactg
     7141 ggtggcgtcg ggataggccg cgctggccac ccgggcgatg ccggcgatgc ccggctcggc
     7201 gcagccggag tggtagaaca gcacgccgtc gcccaccgtc atggcgtcgc gcatgaagtt
     7261 gcgcgcctgg tagttgcgca cgccgtacca gggcacgatc tggtccggcc gggcggccag
     7321 atcgtcgatg gacacgtcgt cgggttccga cttcatcagc caaaaacgca tggaatgctc
     7381 ctgctttacc ggatttggaa gctcggggcc tagggtatca tcggctccgg tgtcctgcgc
     7441 ccctgcgcca ggacggcccg tcgccccctt ccggagcccc ctcccatgag catcccgacc
     7501 gaacgccgca agaacctgca cctgcgcgac ctggtggaca aggcctacgc cctcatcgag
     7561 ccctttttcg atcccgccaa tgcctggaac ggccagaccc tcgaacacct ggcctaccgg
     7621 gtggtccgcg agaacctgcc gggcattccg ccggaggacg tgcaggtggt ggtgagcgcg
     7681 gccacacgca tctaccgcgc caagatcggc ccccagtgac acccccgcag cgtcccgatc
     7741 gccccggcgg cgtcgctgtc cgccgggaat ccgcccgttg acccgcctgt accccctgct
     7801 cttcgtgctc ctgtggagca cgggcttcat cggcgccaag ttcgggctgc cctatgcgga
     7861 gcccctggtc ttcctgtgca tccgctacac gctggtgatc gccctgatgg gcgccgcggc
     7921 gctgcttgcc cgcgcgccct ggccgcagga tccgcgccag tggctgcaca tcggcctcac
     7981 cggcgtactg gtccatggcg tctacctggg cggcgtgttc acggccatcc gtcacggcct
     8041 gcctgcgggc gtcacggccc tggtggtggg cctgcaaccg ctgctcaccg ccctcggcgc
     8101 cggcgtcctg ctgggcgaac gggtactgcc gcgccagtgg gccgggctgg tgctgggctt
     8161 cgccggggtc ggcctggtgg tggcccacaa ggtggcggcg gcggccgacg cccacggact
     8221 caccgcgatg ctggtgccgg ccctggcggc gctggccggc atcaccgtcg gcaccctgta
     8281 ccagaagcgc ttctgcccgg ccttcgacct gcgcaccggg gcggtgatcc agttcgtgcc
     8341 cagcctcgcc ctgtcggccc tggtggcctg gcacacggaa tccctgacgg tggcgtggac
     8401 ggggtccttc gtctttgcgc tcatgtggct ggtcctggtg ctgtcggtgg gcgccatcag
     8461 cctgctcaac ctgctgatcc gcagcggcag cgcggtgaat gtggcgagcc tgttctacct
     8521 cacgcccccc accacggcgc tgatcgcctg ggctgccttc ggcgagacgc tcaccggcct
     8581 ggccctcgcc ggcatggggc tgaccgcctt cggcgtgtgg ctggcgcgtc gatgagcctc
     8641 accaccgagc gcgtcgccac gcgcattctg ggcatcgacc cgggcctgcg catcaccggt
     8701 ttcggcgtca ttgacacgct gggcagcgag ctgcgctacg tggcgagtgg ctgcatccgc
     8761 agcggcgagg gcagcctgcc ccaacgcctc aaggtgatcc tcgacggggt gcgggaagtc
     8821 atcgccacct accggccgga ccaggcggcg gtggagatcg tcttcgtgaa tgtgaatccc
     8881 cagtccaccc tgatgcttgg gcaggcccgc ggcgcggcga tctgcggcgc cgtgtcctgc
     8941 gaattgccgg tggcggaata caccgcgctg caggtcaagc aggcggtcgt cggtcacggc
     9001 aaggccgcca agcagcaggt cagccacatg gtggaacgcc tgctgaccct ggaggcgagc
     9061 ccttcgcccg atgcggccga cgccctcgcc tgcgccatct gccacgccca tgccagcgcc
     9121 gggctggctg ccctggcggg tgtcgggccg cgcaaacggg gcgggcgcct gacgcgctga
     9181 gacgtccacc gcgtaaagac atgcaaatgt cccgccacgg cctctgctgg cacgctatcg
     9241 tagagggcgc gcgggcgtgc tgatgccccg cccgtccgcc tttccgcccg ccgaggcagc
     9301 cctcatgtcc gctcccgccg ccctcccccc cgacgccctg cgcgaacacc gcctgagcct
     9361 ggccgaagtc accgacctgc tggtcgccga ccggctggtg gatcggcccg acgccgacga
     9421 tttcctcaac ggcccggccc gcctgcgcgg tgagatccat cccctggtcc tgctcgccga
     9481 gcagcagtgg accgctcgtg gcgccccgtc ccgcccgctg gacctggaga ccctcaccga
     9541 atggctcgcg ggacgggtca gcctggacta tttccgcatc gaccccctca agatcgactt
     9601 caccgcggtc accgcagtga tgtccgccgc ctacgccgcg cgctttgcga tcctgccggt
     9661 caaggtgacg ccccaggaag tggtgatcgc cacggcccag cccttcctgc gggaatggga
     9721 aggcgagctg gccggcatcc tcaagaaacg cattcaccgg gtgctggcca acccgctgga
     9781 catcgcccgc tacctggtgg agttccacaa cctcgcccgc tcggtgaagc aggccgcgca
     9841 gggcaaccac ggcggcggcg tgtcgtcctt cgagcagctc gtggagctgg gccagaccaa
     9901 ccgccagtac gacgccaacg accagcacat cgtcaatatc gtcgactggc tgtggcagta
     9961 cgccttcgag cagcgcgcca gcgacatcca catggaaccg cgccgcgaca tggggctggt
    10021 gcgcttccgc atcgacggcg tgctccatca ggtttaccag atgcccatgc cggtcatgaa
    10081 cgccatgacc agccgcatca agatcctcgg ccgcatggac gtggtcgaga agcgccggcc
    10141 tcaggacggc cgcatcaaga cccgcgtggc cagcggcaag gaggtggagc tgcgcctgtc
    10201 cacgctgccc accgccttcg gcgaaaagct ggtaatgcgc atcttcgacc cccaggtgct
    10261 ggtgcgcacc ctcgccgagc tcggcttttc cgacaccgac gagggccgct ggcacaccat
    10321 cacccggcgt ccccacggca tcgtgctggt caccggcccc accggatccg gcaagaccac
    10381 cacgctctac tccaccctga agcagctcgc cacgcccgag gtgaacgtgt gcaccatcga
    10441 ggatcccatc gagatggtgg agccgtcctt caaccagatg caggtccagc acagcatcga
    10501 cctcggcttt gcggagggcg tgcgcgccct catgcggcag gatccggaca tcatcatggt
    10561 gggcgagatc cgcgacctgg aaaccgccga gatggccatc caggccgccc tcaccggcca
    10621 cctggtgctg tccaccctgc acaccaacga cgcgccctcc gccgtcaccc gcatgctcga
    10681 cctgggcgtg cccgcctacc tgctgtccgc caccctgctg ggcatcatgg cccagcgcct
    10741 ggtgcgcatc ctgtgccccc actgcaagac cgccgcgccc ctcacgccgg acgaggaggc
    10801 ccagtgggat cacctcgtca cgccgtggaa ggctgcccgg ccgagccgcc tgcaccaccc
    10861 cgtcggctgc atggaatgcc gcatgaccgg ctatctgggg cgcatcggcc tgtacgaaat
    10921 cctgctcctg tcgccggaac tgcgccgcat cgtggtgggc agcggcgatc tcgcccaggt
    10981 gcgcctgcag gccatccgcg aaggcatgaa gcccctgcgc atctccggcg ccctcaaggt
    11041 ggcggccggt ctcaccacgc tcgaggaagt cctcaaggtc gccccgccgg ccgaagacgg
    11101 tcacgagcgc atgcccgagg atgggtcggg acgcccctga gcgccgacgc cctcacagga
    11161 acccgccgtc acagcctgct cggccgcggg tgagcggttg acggccaatc cgccatccgg
    11221 cccctcgccc cgggccgcag cactgcgcct cggcccggag cccatccaca gcaagcactg
    11281 tctcgccccc cgccgaacgc cagcctgacg gcccgaaccc gccgggccat cgacgcatgc
    11341 gtccagctgt gccccccatc gaattccccg ggaacaggga acgccttgct ccgggctggg
    11401 gtgtgcgcgc gagcatgcct ccacgccatg ccaggcccgc cctgtgacct tctgtggcga
    11461 ttcccggccc gtgaccttga cccacatcaa cacgcccccg gccaccgcgc atatgctgaa
    11521 cgactattcc atgttgctaa ccagaaggga gaccccctca tgtccgtccg ccgcctcgcc
    11581 cttgccctgt ccctctgcgg cgccaccgcc gcaattgccg catcccccgc cagaccacag
    11641 gaaccgatta cgccccttgc cccgaccaag gtgagcgccc ctgccctggt cgaactgggc
    11701 aagaagctgt tcttcgatcc gcgcctgtcg cgctccggct tcatctcgtg caattcctgc
    11761 cacaacctcg ccatgggcgg caccgacaac ctgaagacct ccatcggcca caactggcag
    11821 gaaggtccga tcaacgcccc caccgtgctc aactccagcc tcaacctggc ccaattctgg
    11881 gacggccgtg cgccggatct caaggcccag gccggcggcc ccatcgccaa tccgggcgag
    11941 atggcctcca cccatgtgct ggccgtcgag gtgatccagt ccattcccgg ctacagcgcc
    12001 gagttcaagc aggtcttcgg cagcgacaaa gtggatatcg acaagatcac ccgcgccatc
    12061 gccgccttcg aggaaaccct ggtcacgccc aattcccgtt ttgaccagtg gctcaagggc
    12121 aacaagaagg ccctcaacgc cgacgaggtc gccggctaca cgctcttcaa ggacagcggc
    12181 tgtgtcgcct gccacaacgg cccggcggtg ggcggtaatt ccttccagaa gatgggtgtc
    12241 gtcgccccct acaccgccag cagcccggcc gaagggcgca gcgccgtcac cggcaaggat
    12301 gccgaccgct tcaacttcaa ggtgcccacc ctgcgcaacg tggaactgac ctacccgtac
    12361 ttccatgacg gcgccgccac caccctgaag gatgccgtcg ataccatggg ccgcatccag
    12421 ctcggcaagc acttcacgga cgcggagaac gcccggatcg tcgccttcct gaagaccctc
    12481 accggtgccc agccgaactt caagctgccg atcctgcccc cctcgtccga cgcgacgccg
    12541 cggcccgaac cgttcggccg caaataagcc cctgagcgcc cgcgcgcccc ggtccgccgg
    12601 ggcgtcggcg cacttttccg ccaccgcggg gctcgatcgg aacgcaaaac tgtcaaaacc
    12661 ctcaacttcg gagcatccgg gccgaaagag actcgttccg tcccgtcgtg cccccgatgc
    12721 cgcataccgc ctcaccgcag gatgtcttcg tcacgcccga gcagctgtgc gtcgggctgt
    12781 tcattcacgt ggacctgccg tggttcagcc accccttcag cttcaacagc ttcaagatca
    12841 agtcggacga gcagatcgcc acgctgcgca cactgggcgt cagccgcttc cgctgcgatc
    12901 cggcccgctc cgacctgccc ccggccgtcg gcgcctccga gcctcgggac gcgcccgcgc
    12961 cgccccctga agcgcctgcc gccgacgatc cggcgctggc cgccaaacgc gaacgcatcc
    13021 tccgtctgca acagcgcaaa cgccaggtca tcgaggtgga acaggccttc gtgaaggccg
    13081 ccaacgtgat gcgcgacatc ggcaagaacc tcttcgcgcg gccgaaggag tgtcttgaag
    13141 aggtggacga gctggtcaac cagatggtgg tggccttcct ggaccagccg gaagtcgccc
    13201 tgcacgtgat cggtgaacac gccggcggcg aggaggccta ctaccacggc ctcaactgct
    13261 cgatcctcgc catgatgctg gccggcgagc tgggcctgcc ccgcgccgcc ggccagaccc
    13321 tcggcgtcgg cgccctcctc cacgacatcg gcctggtgga catccccgac cgcatcgccc
    13381 gcgcccgcca cgaactgacc ccgccggaac gccacctgcg cgagatgcac tgcgactacg
    13441 gcgtgcgcct cgcccgccag atcggccttc ccgagccggt cctgcgcatc atcgcccagc
    13501 accacgaaat ggccgacggg agcggctatc ccaaggccct caagggcgac cagatcgacc
    13561 cctgcgcccg cctggtggcc ctggtgaact attacgacaa cctgtgcaat ccgggcgacc
    13621 tcgccaaggc cctcacgccc cacgaggcac tgtccctgat gttcgcccag cgccgcggcc
    13681 gcttcgacgc ccaggcgctg caggtgatga tccgctgcct gggcgtgtat ccgccgggca
    13741 cggtggtcaa gctgtccaac gacgccctcg ccctggtggc ctcggtcaat cccgaaaagc
    13801 cgctgcgccc gtgggtcacg gtctatgatc cggatatccc caaggacgag gcgatcatgc
    13861 tcgatctgga gcaggaaccc gacatcaaca tcgccaaggc cctgcgcccc atcgtgctgc
    13921 cacccgaggt ctatgagtac ctgagcccgc gcaagcgcgt gacctactac ttcgacgcca
    13981 accggaggcg cggacagtga gtgccgccca cgatgccatc ctcctcgcgg gcgccgaaga
    14041 gctcctgatg ctcgtagacc cggccaccct ggccatcctc gccgtcaatc cggccgccga
    14101 gcgcctcctc ggcctgcgcg ccgaggccct cgtcggccag ccggtcaccg agatcgaatg
    14161 cgcccttgcg gacctgttct tctgggaaga ggtgcgtagc ggcggtggcg ccgacgaaca
    14221 ggtgatggaa ggcctctacc gccgcgccga cggcaccctg ctgagcgccc gcaaatgcgt
    14281 gcgccgtgtg cccgccggca tgctggtgcg ggccaccgac atcggcgccc agaaaaaggc
    14341 cgaaaccgaa ctggccctga tgaccgccca gctgcgcgcc accctcgagg ccacggcgga
    14401 cgggatcctg gtcatcgacg ccgacggacg ggtggtcaac atgaaccgcc gccttgcgcg
    14461 ccagtggcaa ctgcccgaaa ccctgctcgc ccaccgcgac gacgacgccc tgatcgcgca
    14521 catggccgct gcgctggaaa gcccggcgcc cttccgcagc ctgctcacgg accatctgtt
    14581 cgatctgacc gacaccttcg acaccttcgt cttccgtgac ggccgtcgtt gcgagatcac
    14641 ctcgcgcccg gcacgccagg ccgagcaggt gatcggccgc gtgttctgct tcaccgacgt
    14701 caccgagcgc caccgcagcc agcaggccct catcgccgcc cgcgacgccg ccgacgctgc
    14761 caaccgggcc aagagcgact tcctggcgat catgtcccac gaaatccgca cgccgatgaa
    14821 cggcatcatc ggcatggccc agctcctcgg catgtccgag caccgtccgg aagagcggga
    14881 atacatcgac accatcctga ccagcagcga tgcgctgatg ggcatcatca acgacatcct
    14941 cgactattcc aagatcgaag cccgcaagct caccctcgaa cgcatcagct tcgacctgca
    15001 ccagctcgtc accgacgtgg agcgcatgtt ccgccccaag gccctcgaaa agggcctcca
    15061 gttcggcgcc accctggcca ccaccctgcc ccgcgtggtg atgggcgatc ccacgcgcct
    15121 gcgccagatc ctcgtgaatc tggtgagcaa cgccttcaag ttcaccccgc agggggccat
    15181 ccgcatcgac gtggaaccgg ccgaagcgct ccgcggcggt cgcgtcatcg tgcgctttgc
    15241 ggtcaccgac accggcatcg gcatcccggc cgacaagtgc gagcacatct tcacgccctt
    15301 cgaacaggcc gacatgtcca ccacccggcg ctatggcggc accgggctgg ggctcgccat
    15361 ctgccgcatg ctctgcaccc tgatggaagg cgaaatccgc gtggacagtc aggtcggggc
    15421 cggatcccgt ttcgacttca gcgtggccct cgacgtgggc gtcgcggaag aacgcccgac
    15481 cgtcgaaccg ccccgcgccc tgctgcgtcg cgacacccgc atcctgatcg ccgaagacaa
    15541 cccggtgaac caggtggtgc tcgcccgcct cctcagccgc ctgggggcca cccaaccggc
    15601 gttcgcgcgg gatgggcggg aagcggtgga cctgggcacg gcagaccgtt acgacctgat
    15661 cttcatggat gcgcgcatgc ccgtcatgag cggcatcgag gccacccagg ccctgcgcga
    15721 acggggttgt cgcgcctaca tcgtgggctg cagcgccgac accctgccgg acgacatccg
    15781 cctcgccctc gatgccggca tggacgacta cctgaccaaa cctgtcagcc acgaagccct
    15841 cggtgccgcc atcgaacgct ggcgccgcca cctgggcgag acgcccaccg gctgatccac
    15901 cccctgcaaa agcctcagca tttgtaagcc gccacgcacg gaagcggccc gctccgccct
    15961 gccacggcgc cggacacccg ccacgcaccg gcgtggtttt ccccaatttt gcttacaaag
    16021 gcccacaacg cccatcgccg cctgcgccta tgctgccctt gaaacctccc tatcggcgga
    16081 ggacaaggag aaatcgccat gaaacgccac ccgtcgctcg ctgccgccct gctcctgacg
    16141 ctgggatgtg ctgccaccgt gcccgcctat gcccatggcg gcggagaagg gcatcaccac
    16201 ggcgcgtggg gctggggacc gcggcaccat cactattacc cggtccgcga acccgtcatg
    16261 gtcgtcccgc cccaggccta ttacgcgccg ccgccggtgt actacgccgc gccgccgcgg
    16321 ccggtctatt acacgccgca gccctccgtc gtgatcggca ttccgcccct cgtcattccg
    16381 ctgcgctgaa gccgtcgcgc tgccagaagg cctgggcgaa cgcccgcgcg tccggcagaa
    16441 attcgcgaca atccgccgcg aattccgcgt agtgcatcag caactcggca ccgccgcccg
    16501 caagcgggtt gccgggccgc aggcggcctg ccatgcgctc cagcgcgtac gccaccgtct
    16561 ccacgtcgcg gtacgacgcc agccagtcat cgtcccgcat ggacgggagg atcttctgca
    16621 gacgctcggg cagcccgggc gcctcctcca gcagccggta ctgggccgcc gtgaagtcgg
    16681 ccagcggttc aggatggaat tccgcccagt cgcgcgccag gaagtgatcg taaaacatgt
    16741 ccaccatcac tccggcgacc cgccggcgcg ccgtgctgac ccgcgcccgg ctgcgcagga
    16801 atgccgggtg gctgtctgca aacacgtcga tgcgccggtg cagccgtacg cccgacgcca
    16861 ccgccggcgc cagcgcgccg ggcagcggcc ccttgatgaa gtcccccatc aggcccccca
    16921 ggcgatcggc cggatggggc cccgcgagcc acgcatgggc gaggaaattc acggcctcac
    16981 ccctttcccg gccaccgccg tgcccgctcc ggtgctgccg gcgtcccgac gcagacgcgc
    17041 gcccggagcc cacccgtgcg gcacctcgat gcccattcca gatacatcaa cgatcatcgt
    17101 ttctggcatt tcatccaggc actccgccga aaatgaaaaa aatgctcaag ctccctcaag
    17161 ccatgacagg ttcggccgtt acggagcgtc atgatctgga ggtctgccat gactgctgcg
    17221 ccggtgatcc tgctcgttga tgacagtcgg gtttcccgaa tgctgtgcgg cgccatcgtc
    17281 cgcgagcgcc gccccgatgc catcctcgtc gaagccggca acggcgacga ggccctctcg
    17341 tgcatcgcca ccctggtacc ccacctggcc gtgctggacg tgaacatgcc gggcatgagc
    17401 ggtctcgaac tggccgaacg gatccgcaac gaccaccccg acatgcgcat cgccctgctc
    17461 accgccaacg tccaggacag cgtacgccag cgcgcggcgg cactcaatgc agcctttttt
    17521 gccaagccga tcaacgacac cgtggttggc cgcatcctcg acctgctgga gtcgcaccca
    17581 tgagacccct gagcgagctg gagtgcgacg cattgtcgga agccttcaac ctcgcactgg
    17641 gtgaagcggc cgccaccttt gcatcgatcg tacgcgaaga gatcaccctg tcggctccgg
    17701 tggtggagat catcacccgg gacaccctca tcgcccgcct ccaggcggcc ggctgcggca
    17761 atggcacctc acggatctgc agcatcgacc agcgcttcca cgcggacatg cacttcgaca
    17821 gcgacgccct gctgctgttt cccgaacagg gcagcctcga gatcgtgcgc cgcatgatcg
    17881 gtgacgagac gcccatcgag cagatcaccg aactggagca ggacgccctc gccgagatcg
    17941 gcaacatcat catcaacagc tgcatgagca gtctcgccaa cctgttcggc accgaaatgc
    18001 acggttcgct gccggtggtg cgcagttgca ccgccgacac cctgctcggc gtgcgccagg
    18061 ccagcgacgt gatcctggtg gcgcagatca acatgcgcat gtcggcccac gacatttccg
    18121 gctacgtcct gttcatcatg gacgtgccct ccatcgagtc cttcatgcgc caggtggcgc
    18181 ggctcttcaa tctgcccgaa cgggtctgag cgcgtcatga gcagcacccc cgcccttctc
    18241 gatgccctgg acgtcggcat catcctggta gaccgggatg ggcgggtgca gcactggaac
    18301 cgctggatgg tcacgttcag cggactgcct gcccgtgaca tcctgggttc gcgcctgagt
    18361 accgcgttcg gcaccctgat ggacccgcgc atcgcggtag cgatccgcga agccattgat
    18421 ttcggccgct cctcgcacct gtctcaggtc ttccaccctg cgcccctgcc cctgcacaat
    18481 ccggcagatc ccggcgcgcc ccgtctgcgc cagatgatcg acgccagccc ggtggacgac
    18541 ggcgacgggc ggagctgcct gctgcaggtt cgcgacgtca ccgagacggt ccgccgggag
    18601 gccctgctgc gcagccaggc gcggcgcctg caggaggatc tggcgcggct gtcggccgcc
    18661 caggccgagc tgcagcgcag cgaactccgc tttcgcgaac tggcgcgcca ggcgcccgcg
    18721 ggactgttcg agaccgacgc ccagggccgc ctcatcttcc tcaacgaacg ctgcgcccag
    18781 atgctcggcc gcagcccggc cgatctgccc gacgaaccct ggacccacct gctgccaccc
    18841 gatgacgtgg accgcatgac cgcccgctgg cggcgggccg tccagaccgg caaccggttc
    18901 tccgagcagt tccgctaccg ccatcccaac ggctccgacc tgtggatccg cgccgaggcc
    18961 ggtgtcgtgc gggaaggcag cggcatcggc ggcttcatcg gcaccatggt ggatgtcacc
    19021 gaattccacg agcaggtcgt gcgcaacgaa tttcgcgcca atcacgacag cctgacccgc
    19081 ctgctcaacc gcgagcgctt cgacgtgctc gtccgcgcgg ccgtctccgg cgcgcgggaa
    19141 gtcggccaga agatcgccat cctgttcatc gatctggacc agttcaaggc ggtcaacgac
    19201 acccacggcc acgcggcggg tgaccgggta ctcaagtccg tcgccggacg ggtgcgccgg
    19261 gtcgtccgcg gcgaggatgt ggtggcccgc tacggcggcg acgaattcgc aatcctgctc
    19321 cacgacgcgg gcgacgaagc catgctcaag cggctcaccg ccaacatcga gcgttcaatc
    19381 gccctgccca tcccggtcgg caccggtcag gtccagatcg gctgttccat cggcgtcgcc
    19441 atctatccgg accacggccg cgatccgctc accctgctcg cccacgcgga ccgccagatg
    19501 taccgcatca agcgcagccg ctgggaggcc gcggagccgg accgcgatcc ggtcgcctga
    19561 acacccgcgc gccgcgccca ttcatcgccg ccctccgtcc gccggaccac gcccggctcc
    19621 cggcccgcca ggcggggcgg actgcacccc ggacgacggc tccagaagcc attcggcgcg
    19681 ccccagcgga cccgtcaccc gcaccggcag cgtcgccccg cgcaaggacg ccagcgcaat
    19741 gccctccgcc cccggcggca gcgccagcac ggtcaagcgc tgctgaacgt cgatgcggcc
    19801 ggccgccaga tccagcacgc cgtgtccgct cgcccgccac tgggcgctgc gcgcctgcag
    19861 atcggcactg ctcaccacgc cccgctcgat gccgaaactg cccgaccatt ccgtcaggcc
    19921 cgcctgctca gcgtcccgaa acgtccgccg ggccccacgg cgagcggcaa tggctgcctg
    19981 ccattcgcgc agcgccgcga acggatcgat cccccgcacg gcgccgtgac ccagcacaaa
    20041 gcgccccgtg ccggcgaggg cgcccgcgag ggctgatcgc cgcccggccg ccgtcgccac
    20101 atccacgaac gcgttcagcg tgccgctcag ggcctcccgt ccggtggcat cgcgcagcag
    20161 cggctccacg ctgacgccat tgatctggcc ggtgtagaac gccttgttgc catcggccac
    20221 cagggtgaaa ttgcccgcca cggcgccgcc gtacagcccg gcagcatgcc cccgcgacac
    20281 cagccggccg ccgtgcaggc tgaggggcag acgaacgcgc tcaaactgca gccccgcggc
    20341 gcggaggctg ccgatgcgca gctgaccgtc cacatcgaac ccgtgcaggc cggacagatc
    20401 ccagcggtcg cccgttgccg caaacaggcg gtcggcgtcc aggcggtccg cctccagctc
    20461 aaagcgaccg gcccacggcg cgaaccggct caaggcccag gtgccgttga tccggctgcc
    20521 gtcgatgcgc agatcgaccg tcccgccggc gctgccgcgg gccgtatcca cctgaaggtc
    20581 tgcaccggcc cgcaccggac tggacgcgcc accgcgccgc gtccaggttc cttcgagcac
    20641 cacgccacgc agctgtgcga gggactccgc ggcccgccag tcgccagcgc cgcgcccctg
    20701 gaggttcagg cggtcatccc ctgtgcggag atccgccgtc acgctgatgc cagccgctcg
    20761 cgcctgcaga tcggagagac gggccgtcag acgtccgccc tccgcctcgc tcccccagcg
    20821 tgcgtccgcg tccaccgtcg ccgcgaccgg gccgttcgcc gtgaggcgca ggtcgggcgt
    20881 cgccaggtgc aggaccgggg cgccctccgc agcccgtgca cgcagatcgc ccgccgcacc
    20941 cttgaccaca agctcgcctc ccgtcaccca ggccagctcg tcgaggctcg cccgcgcctc
    21001 gaggtctcca cggcccgcca gcacgccgac cgccgacagg ctcagatgac gcagggcatg
    21061 acgcccgccc accgcgtcca ggcgatagtc gctggccacg cggaggtcca cctccacgtt
    21121 cggtgaatcg ccgcgcaggt ggcctgtcag gctcaatcgc ccgaccgact gcgacgccag
    21181 atcgtccgtc tcgagcgtca gcccggtgac gcccaggcga cgaccgcccg cctcatccag
    21241 ccagtccacc cggccgtcat gcacgacgag gcggtgcaca tcggccgtca gcggcaaggc
    21301 cgtgtcgccc tcctggccct gcagcagcgc ggccagatcg tcggtgttca cggccccatc
    21361 gcgtcggcgc cggagcgtga gctgcgggcc ctgcagttcg accgcttcga tcgccacccg
    21421 ccgcgacaac agcggccaca gaccgacacg cagctcggcc cgctcaaacg cgaggaatgc
    21481 cccgtcgctg cgcggatccg acacggtggt ggccggcagg cgcagactca gccggggaaa
    21541 cagcgacaag gccaccggcc cgtccaggcg cacggtacgc tgggtctgct ggcgcaggga
    21601 ctgggtcagc tcagcggcga ggcgggcgcc atcgaaggtc gcatacaggt agagggcccc
    21661 agcccccatc acggcgctca gcccgcccag caggaaagcc aggagcttca gcggtttgaa
    21721 acccatgtcc gggacgtccg tgcttcaccc agcgccgcgc ccggtctcaa tccgggcgcg
    21781 aaggcgcgtc cggacgcatg cgcaggggca gccggatctc gaacatgctg ccgacccccg
    21841 gcgtgctctc gacggacacg ttgcccccat gacgctcggc cacggtcttg acgatgagga
    21901 gccccagccc gacgccgctg ggccggtcgc gcccttcctc cacaatgcgg ctgaagcgct
    21961 tgaacaggcg cggcagcgca tccggcggaa tgccagcccc ctggccgcgc acgcggatca
    22021 cccagtcggc gccctcggcg gcgagctccg cgctcaccgt gctgtccgga ctgccgtact
    22081 tgatggcatt ggtgagcagg ttgatcacgg cccggcgcag cagcgagaag tcgccccgca
    22141 cgatgggctc ctcggtctcc gccgccctcg tgtccacggc gacacggatg ccgcgggcgt
    22201 gggcctgggg ccacacctcg tcggcggcgg aatccaccag accggccagg tccagctcca
    22261 cgaaatcctt cgggtcagac ccctcggcac ggaccagacg gaacaggccg tcagccagat
    22321 cgagcgccga acgggcgtag cgggcgatgc gcggaatctg ttccgcctgg gcggccggat
    22381 cacccttgta gccgtcgatg agcgtcagga tggaggccag cggcgcgcgc aggtcgtggg
    22441 acaggaaatg catggtgtcc tcccgcgtac gctgggcctc gtgcatgacg gtcacatccg
    22501 ccagaccaat cacgcggccg gacaggctgc ccgtaccggc caccagctcg gccaccgaca
    22561 ggagcagatg gcgccgcccc acgggcagct cgaactggcg gatgccggca aacccggcca
    22621 ggtccggcag gccgtcgcgc atcggcagat acacgggcca cggcaggctg cgcaaggccg
    22681 ccagcaggtc ttcgccacgg aaggcctgga gcagatggcg ggcacgctca ttgtgcagca
    22741 ccacccggtt gcccggatcg atgaccacca caccgatggg caggtgctcg agggtgtcgg
    22801 caatgaagcg gcggacatcc cgctgctgcc gggccacgtg gcggacgatg cggatgcgcc
    22861 gctccaggcg atccgccgcc ccccgatcca cctccgacgg cagtgcgtcc ccttctcgct
    22921 ccagcgcggc cagctccgca tccagatagc gctgggccca ttccaggcgg cgccagctcc
    22981 acaggggata cgccagcatg cagcccagca ccgcgggagc cggcccgaac cacatgctgc
    23041 ccgcttgcag cgccacggcc gccagggcca gcgcggccag cccggccagg acggacacca
    23101 gcagtccggt gcgcggatcc ccgtagaaca gcgcaagcat cgcgaccagc accgccaggg
    23161 ccgccacacc ggccgaggtc cccgccgaca gcggccggac ggcatctccc cggcgcaagg
    23221 cgtcgtaaac gttggcgtcc acctcgacgc cgggcatggg ccgcgacagg ccggacatgg
    23281 gcgtcgggac ggcctcggcc atccccgagg ccgtggcgcc caccagcagc accttgtcgc
    23341 gcagggcgtc ggcgggcact tcgccccgca gcaccgcggc gtaggaataa tagggatagg
    23401 tgcccggcgg cccggcgaag ggtacccgca gccacgtggc gcgcacccat tgcagacgat
    23461 cgtcgtcatc gccggggccc ggctgcgcat agcgggcggc cacctccgga tcgacgatgc
    23521 ggagcagcgc cagcgccaat tggggatggc gggcactgcc aaagccttcc cacagataga
    23581 cgctgcgggc gatgccgtcc ggatcgaact ccacctgccc gtggccgagt gcagccgccg
    23641 ccgcgacgaa ttcgggcagg ggcaggatct cgccggtcag caggacgccg gtcgtggcct
    23701 gggtgagggg cagggcgacc cggccgaagg cccgcatgga ctgggccagc accgtgtcgg
    23761 ccgccggatc gtcccgcgac ggggtgtcca ggatcaggtc cagcgccacc gcccgcgccc
    23821 cggcatcgtg cagacgatcg aggagcgccg catgcacccg ccgcgaccag ggccagcttc
    23881 ccagttcggc caggctcgcg tcatcgatgg ccacgatcgc cacgttggca ctcgtggccc
    23941 gacggaagct ggacaggaag ctgtcgtaga tcgtctgatc gatgcgccac agccagccct
    24001 caagggccgc aaacaccgcc agcacgatgc acagcgtcgt gacggccagc cactccagtg
    24061 tcggccggcg caagacggtc ccggttcccg acttcacggc gccgcgttcg acagggtcca
    24121 catcagcagg ggcagcctgt aaagaacctg caccggcgtg taaggactcg ccgcgccctg
    24181 ggcgtcggtg ccgcgcacgc ggacgaaata gcgcccgccc gccggcaccg gcacggtgat
    24241 ggccgcctcg cccaggcgct ggttgaccac cagatcgccg aacccggcgt cgcgggcgag
    24301 ctgaaaatca tagatctgac cgtcgtcgcc gctccattca aatcgcaggc ggtcgttgta
    24361 gcggcggacc gtgggcacac cgatggggcg cgtcaccgcc aaggcacggg cgtcgcccca
    24421 cggaccctgc ccaccggcag gcttcagggt ggccacacgc cagcggaact cgccccgcgg
    24481 caaggtcaga tcgatgcccg gcgtaacaac ctcccgatca atcagcagca cctccggcgc
    24541 agcacgccgc agcacctgga agcggtaacc cggtgcatcc gccaccggcg tccactcgaa
    24601 atggaccggc ccgccgacga aggccgcgcc ggccaagggc ccggcagcgt cgggcggcac
    24661 cggccgggcg cgcacttcca gggcacgctg ggtgtcgaag ccttcgagtc cggccgcgtc
    24721 gatggcgcgg acgcgtaccc acaggcgccc gtccggaacg ttttcgaggc gcaccggcgg
    24781 cgccggcgat acggcctccg tcaccacgtc ggtgaatgcc gcatcgcgag ccagctgggc
    24841 acggtaggcc accgccttgt ccacaggccg cagcgtgatc tccacggcgg tacgttcatg
    24901 gacggtcgcc agaccttcca gcgcgggtgc cggcagcagg gcttgcggcg gctggacgcc
    24961 tccaccaggc tcggcggtga ccccgaagcc catggccacg gcggtggccg gcccggtctc
    25021 ggcgccgacg cgcacctcac cgccggtcac ttcggcgcgc gcgcgcccat cggtcccatc
    25081 ggaacccacg cggaattcgg tgccccgcac ggatacgctc gcactcggcg tgcggatccg
    25141 gtaacgggcc gccgggccac gctgggccgc aaccgtattc tcgatgcggc cccgttcaag
    25201 gtggatctgg gccgtctggc cggtgagccc ggcaaacccc tggatcttct cgaggcgtgc
    25261 ctcgctgtcc ggctggaggg tcagacgcga ttcgtcgggc atgcgcagcg tgacgaagga
    25321 cgcgttcccc gtccgcagca ccgccccggg cggcacctga tcctccaggg ccaggggacg
    25381 cccgttgagg gtggcctccc catgcacgcc caccacctgg gcggatcgcg cctcgacccg
    25441 cagccaggct tcggggatgc gcacctgcgt gccggtggga atgcgccgcg gctcggcgat
    25501 gcggttgtgc agcatgaccc ccttccagcc aaacggtgcg gcgagcagct gtttctgcaa
    25561 gccgatcagg gtatcgcccg ggcgagccgt gtagacaatg tcctcgtccg cggcgaacgc
    25621 gccgccgctc caggccagga cgcccagtac ggcggacagg atgcgatgtc gatgaagggc
    25681 caaggtcagg tctcgcggat cggtcgaagg aacaaaatat agtcggttcg ccgaggtcgg
    25741 gcgatgtgaa acttcgcaaa ctggccttaa aaaggtgaag aacggtgccg tattcccgcc
    25801 gctccatggc ccgtatcgct atgatcggca cggctttccg ggtaggcgcc gtttcgggcc
    25861 gtagtctacc cgtccccttt cccccgacac gaggagaata gcgtgagcat catccaggag
    25921 ttcaaggaat ttgccctcaa gggcaatgtg gtcgatctgg cggtcggcgt gatcatcggc
    25981 ggcgccttcc agaagatcgt cgactccctc gtcaaggatc tgctgatgcc ggtgctgggc
    26041 aaactcctcg gtggcgtgga cttcaagcac ctttacatca acctgggtga caagacctac
    26101 gagacgctgg acgccgccga gaaggcgggc gcccccttgt tcaagtacgg cgtgttcatc
    26161 aacaccgtga tcgacttcgt gatcgtcgcc gccgccatct tcgtcgccat caaggcgatg
    26221 aacaaactca aacgcgaaga acccgtgccc gcgccggaag caccggtcga ggccgaagac
    26281 gtgaagctgc tgcgggaaat ccgcgatgcc ctgaagaaat aggacatcgt ccggtgtgcc
    26341 agcgggaccc gcccgggcgg gtcccgccgg ttcagacctg ggaagacagg tcggcgcctt
    26401 cggcgggcgc accgtccggc gcgaggttcg ccgcatcgac ccgctcgagg cgatagccga
    26461 agttataggt gggggtcagg cggaacccgt tttccggccg cagattgagc ttgctgcgca
    26521 cccggctgat atgggtatcc acggtccggg tcgccagatc ggggttacgg ccccacaccg
    26581 cttccagcag atggccgcgg gacaggagac ggcccatgtt gcggaacagg aaggccgcca
    26641 gttcgaactc cttgtcggtc atctcgaccg gctcgccatt taccgtgacc atcttgcgga
    26701 tcaggtcgaa gctgtagggc gcgacctgca gcagctggct gacctggcgc ggctgggtcc
    26761 ggcgcagcac ggcctcgatg cgcgacagca gctcgatgcg ccgcggcggc ttgaccagat
    26821 agtcgtcggc accggcgctg aaggcgctgg cgatgtcatc ttcggcatcc cgcgacgtga
    26881 tgaagatcgc cggcgtatcg ttgttgcgct cggtgcgcaa ccagcgcagc acctgctcgc
    26941 cggtcagatc cggcagcacc cagtccacca ggaagagatc gaaactctcc cggctggcca
    27001 ccctcatcag ttcgcgcgcc atggcaaatc catgcacgtc gtgcccggct tcgcgcaacc
    27061 agccagtcag cacttccatt tgggcaggat cgtcttcgag aatagcgaat cgcacgctgg
    27121 ctcacctttt caataaatgt gcaacgtcaa atcggcaccg gaatcccctg cctgcgggct
    27181 gcgaccggca tttcaggcgt aatcccccac atagggattg gcgtcccgtt cgtcgccgaa
    27241 cgtggacatc ggcccgtggc ccggaatgaa ggccacatcc ttgcccagcg gccacagacg
    27301 agtccggatc gaggcaatca gtgcggcgtg attgcctcgt ggaaaatcgg tacgcccgat
    27361 ggagccggcg aacagcacgt cccccaccac cgccagacgt gccgaaggac tgaaaaacac
    27421 cacatgcccc ggggtatgac cggggcagtg gatgacatca agggtttcct caccgacctg
    27481 gacggtgtcg ccatcggcca gccagcgatc gggcgtgaag gcgcccaccg ggggaaagcc
    27541 gaacatgcgg ctctgctggg gcaatccctc gatccagaag gcgtcctccc gatccgggcc
    27601 ctcgatgggc acggccagtt gccgcgccaa atcggcggta ccgcccgcat gatcgatatg
    27661 accatgggtc agcaggatgc gctcgacggt gacgcccagc gcctggacgg cagccaggat
    27721 ccggtccaca ttgcctcccg gatcgacaac ggccgcctga cgacttgcgt cacaccagag
    27781 gatcgagcaa ttctgctcga aaggtgtcac gggaacgatc ttgtattgca acatgagcag
    27841 tcgggcaatc ggcagcgttt ctgaaaccgg tcgggccggg cacgcgggca atggcttgac
    27901 cgccttcacc caccatgaca tcagcggagg agtttatcac gcagccttcg ggcgaccagc
    27961 gtccgcggac catcccaaaa ggatgtcagc ccctatcgac acgcttgttt tcatttaatg
    28021 aaaagcagcc attccacccg cggtgcgttt tagcgcccgg aagcaccgcc ggcagaatgc
    28081 gcctcccggc gcctgctcac gcccctcgca ggggcgccgc caattgatcc cgaccggcga
    28141 tgcaattcgc cggagcgccg tgtcctattc ggttaccggc gtcctgtcga aataattgaa
    28201 aaggaatatt gcttcgcagc gctcagcgaa tatcgggcgc gcgcggatcg gtcagggcaa
    28261 tgtagcgggc ccgatcctgc tcgtatcggg cgcggatgtt ctcgatgtct cggcgcttga
    28321 cctcgatcac cgaccattgg gcggccagct cgctgtcgga ttccctcagg gaattggcca
    28381 gggccaccgg catggatcgc ttctggtaga actccgcctc ccgcgtcagc ccctgccggc
    28441 gggcctgcaa ctggacataa cgcgcttcgg cctgcttcag ctcggactgg accgactcga
    28501 tggccttgtc acggcgatta tcgatgtcct gcacgctgga ataggtttcc agcagggact
    28561 gattgcgccg tgtttcggcc cgctggcgtg cctgctcggc cttgcgggcg cgctcctcgg
    28621 cctcccggcg ggcgagctgt tcgggcgtga ggggcgcctc caccagacga cgcacgccgc
    28681 cctgtgggct catttcacgg tagcttttgc catagcactg ctccggcagc acatccccgc
    28741 agatctgatg cccgctggcg tcgggacagc agaacacccg tggcgccttg gcggacgact
    28801 gggcgccggc gggcaccgac acggcggcga gcagacagaa aagcagccgg cgcatcatca
    28861 cggctcaggc ctcccgcgcc acgccatagg tttcccggta acgcgtgacc gcgcccaggt
    28921 tttccgcaag ggcagggctg tccttgaggt aggcgatgag gtctgccaga ttggccaccg
    28981 ccagcacggg aatgtcgtag gtgcgctgaa cctcctggac ggccgacagg ctgccggtgc
    29041 cccgctccat gcggtcgagg gcgatcacga ccgctgccgg cgtggcacca tgggcgcgga
    29101 tgatctcgac ggattcccgc acggaggtgc ccgcggaaat cacgtcgtcg agaatcgcca
    29161 cccgcccggc cagcggcgcc cccaccaggg taccgccctc accgtggtcc ttggcttcct
    29221 tgcggttgaa gcagaatggc aggttcacgc cccgctcggc catccgcatc gccgtgccgg
    29281 cgaccagcgg aatgcccttg taggcaggac cgaagagcat gtcgcagggc agatgcgccg
    29341 cgatcagcgt gcgggcgtag aagtcgcaca gacggccgaa ggattcaccg tcgttgaaca
    29401 gccccgcatt gaagaaataa ggcgacagac ggccggcctt ggtgacgaac tcgccgaaac
    29461 gcaagacgcc cttgtcacag gcaagctgga tgaaatcacg gctaaaatcc actggcaatc
    29521 caacccataa accggtccaa attaagtcaa atgttacgcg tcgttacagc caacctcaac
    29581 ggaattcgct ccgcaaacgt gaaaggcttc ctcccctggc tcgctttgca gcaggccgat
    29641 gtggtctgcc tccaggagct gaaagcacag tccgccgacc tgccgccgga aacggtcacc
    29701 cccaatggat ttcggggatt cttttccttt gccgagaaaa agggctacag cggcgtcggc
    29761 atatatgcaa aaaaaattcc cgaccaggtc atatacggat tcggcattcc tgaattcgat
    29821 gccgaaggtc gctatatcca ggccgatttt ggcaatgtgt cgattatttc actttatctc
    29881 ccgtctggtt ccagttccga cgaacggcag gccgccaagt tcaggttcat ggcgcacttc
    29941 ctgccccacc tggcgcagct gcgctgcagc ggtcgggagg tgatcgtctg cggcgactgg
    30001 aacatcgccc accaggccat cgacctcaag aactggcgct ccaaccagaa gaactccggc
    30061 ttcctgcccg aagagcgggc ctggctgtcg cgcctgttcg acgaacaggg ctgggtggac
    30121 acctaccgcc gcctccatcc cgacgccacc gatgcctgct acacgtggtg gtccaaccgg
    30181 ggccaggcct gggccaagaa cgtgggctgg cggctcgact accagatcgc cacccccggc
    30241 atcgccgcgc aggtccggcg ctgcgccatc tacaaggagc agcgcttcag tgaccacgcg
    30301 ccgctgaccg tcgattacga ccacccgttc ggagactgaa gcccgcagcg gcgtttaaat
    30361 aggcaccgac tatcttaata ctaacaacaa tcaatttcac acatcgagac ccccgccgta
    30421 gcatctctcc atcgcaagcc tgcgacactt ttcaaggaga gacatcatgg caaacccctt
    30481 ccacgacccc gcctcggcca ccgtccagaa cctcgaagcc gccttcgccg gcgagtcgat
    30541 ggcgcacatc aagtaccgct atttcgccaa gctctgccgc gagatgggcg acgaggagac
    30601 ggcccgcgcc ttcgaggcga cggccgacca ggaggtcatg cacgccttcg gccacctgga
    30661 cctcctctac ccgaaggcga agatgagccc cgcccgcgcg ctcgaattcg ccatcgaagg
    30721 cgagacctac gagtacaccg agatgtaccc ggccttccgc cacaccgccg tcgccgaagg
    30781 ccatagcgcc gcggtgcagg aaatcgacga acagatcgcc gaatcccgcg agcacgccga
    30841 gcagttcaag gccgtcctcg aaaaggccgc caagcgcttc gccgccctcg ccaaggtgga
    30901 agagcgccac gccgcccact accaggcgcg ccttgaccag atcgacgcct gaacccaccc
    30961 cctacctgag acaaggaatc aagaccatga aaacctggca atgcatcgtg tgcggcttca
    31021 tctacgacga aaccgcagga cttcccgaag aaggcatcgc gcccggcacc gcctgggccg
    31081 acatcccgga cgactgggcc tgcccggact gcggcgtcgc caagtccgac ttcgagatga
    31141 tcgagctctg acccgctggc gcccgccccc gcgcgggcgc cgcactgcac cggacgccca
    31201 ccatgaccgc ccccctcctc atcctcggca ccggcctcgc cggctacacc ctcgcccgcg
    31261 aagtccgcaa gctcgaccgg gaccggcccc tcgtgctcgt cagccgcgac gatgcgccct
    31321 tctactccaa gcccatgctc tccaacgccc tggccgccgg caagagcgcc accaccctgg
    31381 tgatgaagtc cgccgagcgg atggccgagg aactgggcgc gacgatcctg ccgcgccgcc
    31441 gcgtggcgtc cctcgacccg gccgcgcggc acgtgcagtt cgatggcggc gagaccctcg
    31501 cctacggcga cctcgtcctc gccctcggcg ccgacccgat ccgcctgccg ctcgccggcg
    31561 acggcgccga cgacgtgctc tcggtgaacg acctggacga cttcgcccgc ttcagtgccc
    31621 gcctggacgg cgtacagcgc gtcgtgattc tcggcgccgg cctgatcggc tgcgaattcg
    31681 ccaacgacct cctcgcccgc ggcatcagcc cgagcgtctt cgacgtcgcc ccgtggcccc
    31741 tcggccgcct gctgccggac gccgcaggcc gccacttcca ggcccgcctc gaagccgccg
    31801 gcgtacgctt ccacctgggc caggccgccc aggccgtgca tcgggacgga cccggctacc
    31861 gggtcgaact gggcgacggt cgccaggagc cggcagacct cgtcctgtcc gccgtcggcc
    31921 tgcgcccgcg caccgcgctg gccgctgccg caggcctcac cgtcggacgc ggcatcacgg
    31981 tggaccgccg cctcgccacg agcgacccgc acatcttcgc cctcggcgac tgcgccgaag
    32041 tggacggcct tcacctgccc ttcgtgctgc ccctgatgca gcaggcccgg gccctcgccc
    32101 gcagcctgac gggcaccccc accgacgtga gctacccgcc catgccggtg ctggtgaaga
    32161 ccccggcgat ccccaccatc gtcgcgccac cgcccatcgg cgcggcgggc gtctggcgga
    32221 tcgtcgaaga caccccggac gcaggcctcg aagcccgctt cgaagccccc gacggcagtc
    32281 tgcgcggctt tgcgctgctc ggtcccgcca ccgcccgcaa gcagaccctc agcgcagccc
    32341 tccctgcact gctgtaggcc ccggcgcggc cagccgcccg gcgtacccga tcgggatgcg
    32401 ccggggtata gtcggagcct tgtccgcccg gagcgcgccc atggtccctc ccttcacccg
    32461 ccctgtcctc cgcctcctca tcctgtgggc cggcctgctg acggccctcg ccgcgctgcc
    32521 cgccttcgcc gcacccgcgt cgtcccaccc acaaaaagtc cagtccgccc cccccgacct
    32581 tccgcccgcc gccgacagcg cccctgtcct cctgggcggc gaagaggtct ttcgcatcgc
    32641 cgccccgttc aaggcctatt caccggccgt ccgcgcccgc cagatctccc gccgcctcgc
    32701 cgatctggcc acccggggca ccgcgcccga gatccgcgtc gaggaatcgg acatctccag
    32761 cgaccttctg gccgacgatc aggtcctcct ctccgttttt gatgcggacg ccgccgccga
    32821 gggcattccg cgtgccgacc tcgccgcccg ccacgccgcc accctcggcg cagccctcgc
    32881 ccgctatcac gacgaacacc gccacgaagc cctcctcatc ggccttggca aaagcgccgg
    32941 ggccgtgctc gccgccctcg ccctgctgac cctcttcgag cgtggctacc ggcgcctccg
    33001 tgaagccatc ctgcgcaagg ccgacgagac catcgccagc gtcgagcgca ggagcttcag
    33061 cctggtccgc ggcagctacc tgcgcacccc gctgctggct ctgctccatg gcatccgcct
    33121 ggccgtgtgg gccagcctgc tgtacgccgc cctgcacctg tccctcgcct ccttccccca
    33181 gacgcgacgc ttcgccaccc atctgtcgga gctggtcctg acccccctcg gcaccctcgg
    33241 ccacggcctc ctcgacgcca ttcccgggct cctggtggtg gtcatcatcg gggtgctcgc
    33301 ccgctggctg atccgcagcg ccagcttcat cttcgagcgc atcggcgacg ggcgcatcgt
    33361 cctcgacggc ttctacccgg aatgggccgc accgacccgg cgcatcgtgc atctgctcct
    33421 catcgccggc gcggtgatga tcgcctaccc ctacatcccc ggctccgact ccgccgcctt
    33481 caagggcgtc tccatcttcc tcggcgtgct cctgtccctg ggctccagcg gcgtcatctc
    33541 caacatcgtc accggcttca tgatcaccta catgcgcgcc ttcaaggtgg gcgacgtggt
    33601 gcgcatcggc gagaccaccg gcatcgtggt gagcagctcc ctgctggtca cgcgcctgcg
    33661 caccatccgc aacatcgaga tcaccgtgcc caattcccag atcctcaacg gtcaggtcac
    33721 caacttcagc gtcaccggca atcccaccgt ctcgacgcaa gtcaccatcg gctacgacac
    33781 gccctggcgc caggtgcacg ccctgctcct cgacgccgcc gcgcgcacga ccggcatcgt
    33841 ccaccagccg ccgcccttcg tcctgcagac cgccctcgaa gatttctaca tccgctatga
    33901 actcatgtgc gtcgtagaca atccgacacg catgcccttc atcctctccg ccctgcacca
    33961 ggccatcctc gacgccttca acgaatacgg cgtgcagatc atgtcccccc atttcatcga
    34021 caaccctggc aacccggccg tggtgccacg cgaacagtgg cacgccgccc cggcgcggcc
    34081 gcctgcctga gccggaccct gcgccagatc aagggtccgg cgttttaccg gtcccttcaa
    34141 attgccttcc cgatcgattg ggattagcct agaaccgatg ccccaggcgg cccagaccgt
    34201 cccgcgccac ccatcacctt ccttcctgaa acaagacgcc cggagaacac catgagcgaa
    34261 acccaagtca ccaaggacaa actggttgcc gatttcaagg ccgtcgtcgc cgacaccgaa
    34321 gagctgctca agctcaccgc cggcaccgcc ggtgacaagg tcgcagatgt gcgcgcgcgc
    34381 ctcaccgaca agctcggcac cgccaaggaa aagctccagg atctcgaagc cgccgtggtt
    34441 gccaagacca aggccgccgc ccgcgccacc gacgactacg tgcacgacaa cccgtggaag
    34501 gccgttggcg tcgccgccgg ggtcggcttc ctgctcggcc tgctggtcaa ccgccgctga
    34561 accggcgcat gagcgactcc gcgccgccgc gcctcggcga gtccctcagg gggctcgccg
    34621 acaccggcct gggcgtgctc cagacccggc tcgagctctt cgccgtcgag ctgaaggaag
    34681 agaagctgcg cgtcggcagc ttcctcttcg acaccgtgct ggccgccctc ttcatcggct
    34741 tcggcatcgt cttcctgctc gccttcctca ccgtgctgat gtgggacgag catcgtctgc
    34801 tggcgctcgg cctcgccacc gccgggctct tcgcggcggg catctatgcg gcgacccgcg
    34861 cggccacccg tctgcgtgcc ggttcgcggc tcttcgccgc cagtctggcc gagctggccc
    34921 aggaccggga cgctttgcgg cccgaggact gaccccacgc atgagccccg aactggtcga
    34981 acttgccctg cgcaagcagc gcctgcaaat ccgttccgcg gcccagcgtc aggcccttgc
    35041 cgggcaccta gcggccttcg caccgctgct gcggggggcc gacgccgtga ccgacggtct
    35101 gcactgggcg cgccgccacc ccgccctgct cgccggcgcc gcagcctttc tgcttgtcac
    35161 ccgcccccgc cgcgccgtgc gctggctgcg ccgcggctgg ttcggctggc agctcgtgcg
    35221 ccgcgcccgc gccctggtct cctgaacctc cgaaccatga ccctcgatct cgacgacatc
    35281 cgccgcgtcc gcgccgaagc cgactgcctg tgcacaccgg ccgaggtgga cgccgccctt
    35341 gaccgcctcg ccgccgccat caccgaacgc ttccacgacc gcaacccgct gctctacgtg
    35401 gtcatgaacg gcggcctggt cctcgccggg cgtctgctcc atcgcctgga cttcccgctg
    35461 gaggtcgcct atctccacgc cacccgctac ggccacgccc tgcagggcac cctgctggac
    35521 tggcgcgtgc gccccaccca ggacctgcgc gaccgtcacg tgctggtgct cgacgacatc
    35581 ctcgacgaag gccacaccct cgccgccatc atcgactacc tgaaatccga aggcgcccgc
    35641 gacgtccagt ccgcggtgct ggtgcacaag ctgcacgacc gcaaggccta ccccggcatg
    35701 cgcgccgatt tcaccggcct ggacgtggcc gaccgcttcc tgttcggctg cggcatggac
    35761 tacaagggct actggcgcaa cgccgccggc atctacgccc tcaagggaca ctgaggcgca
    35821 ccgcaggccg agcgccgcgg ccccgcgccg ggcgctcaca cccggaagcg tcccgtctcc
    35881 gcctgcagtt ccgccgccag cccggcgagc tggcgcgccg ccaccgccgc atcggcaatc
    35941 tccgcgccat tgcgctcggc cagctgggcg atgccctcca cgtccgccgc cacccgcccg
    36001 ctcacgtcgc gctgcacacc cgtggccgcc gagatctccg ccgtgccgcg caccgcatcc
    36061 tccaccgccc gattcgcctc gcccagggcc tcggccaccc ggcgcatgga ctcccggctc
    36121 gaacgcagca cctccagccc gtgctggatc gccgtccgcg ccgcgctgga atcctccccc
    36181 agggcgccgg tgatggtgtc gatggccgcc gccgaccggt gcgactgctc cgccagcttg
    36241 cgcacctcgt ccgccaccac cgcaaaccca cggcccgatt cgcccgcccg cgccgcctcg
    36301 atggccgcgt tcagggccag cagattggtc tggtccgaga tccccttcac ctgctgggtc
    36361 atggcggtga tggactccgc ccgattcacg aagttttcca cggtgccgac gatctcctgc
    36421 accgcccggt cgatgccgtg cagcttgtcg tccagctccg tcagggtctc gttgccgtgg
    36481 tgggtgcagg tcatgctgtg ggtcgccagg gcctgcaccc gcgtgctcag atccgccacc
    36541 tgggcaatgc tgcccacgat ctcctccata cccgccgccg ccgacgccga acgccgcgcc
    36601 tgctcggtgg agccgtccgc gatgcgcgcc gtgccccgct ccagggccga cgccgccccg
    36661 gccacgtcgc cggacgagcg gatcacctgc ccgatcaggc gctgcagcga atccatcatc
    36721 cggttgaagg ccgccgacgc cagccccacc tcatcctgac gccgcgcctt gaggcgccgg
    36781 gtcagatccc cctcgccgcc ggcgatgtcc gccagattgc gggtcatccc gttcagcgga
    36841 cgcgccagcg cattgcgcac aaagacatac accgccgcca ccagcaccac ccccagcagc
    36901 aaggccaccg ccaccgtggt cagcaacgac tcccgcagat tgcgcttcac cgcatccagc
    36961 tgcacgctca tggagaccgc cccgagcacg tcgccctcct tcgcctcgtc gtggcattcc
    37021 aggcactcct tgcccaggta gcgcttctcc gcgatggccg gcaacaccgc cttcagcacc
    37081 ggctggccct cctcctcgcg gatctcgtag cgcgccttgc catcggccag caccgccttt
    37141 tccagcggat cgtccgccgg aatcaccccc tgctcccgct cgccgaactg ctggaccgtc
    37201 gccgccccgc gcagcacctt cagatgccgc agcccgcggg actgctccac ctgctccagg
    37261 taatagccct ggcgcttgta gttgcgcgtc gccttggcga acatcaactg cgccatcgtc
    37321 atctgatgca ccgactgggc gaactcctcc gcctgctcga tggccgtgtg ctgctgttcc
    37381 cgcgccaccc accacaacac ccccgccgat cccagcgcca tcagcgacgc gatcacgatc
    37441 accaggcgga cccagatctt ctgcatgttc aggttcatga aatccctcga caaagtggtc
    37501 ggcgccccgt ctcggcgggg tctccgagcc gcctaacggc gcgggcaggc aatagttgag
    37561 atgcgataag gggttttgct gcccggaccg gtccccgacg cccgtccccg actggccgcc
    37621 ctcgcgccgg ccggcctata cttgcccgcg tcatcccccg tctgcccgcc gtcatgaccg
    37681 acgccccccg ctcgccgcgc agcctccgcc tcttcctcag ctcccccggc gacgtcgccg
    37741 acgagcggca ggccgtccgc cgcgttctcg aagacctggc cctcaccccg ctgctgcgca
    37801 cccgcgtgcg cttcgacctc gtcgcctggg acgaccccgc cgcccccgtc cccctggacg
    37861 ccaccgaaac accccagctc tccgtcaacc gctacaaagg cctgccccgc gactgcgacc
    37921 tcaccgtcgt catcctgtgg agccgcctcg gcacccccct gccgtcccac gtcacccgcc
    37981 cggacggcag cgcctacgcc tccggcaccg tgtgggaact ggaagacgcc cgcaccggcc
    38041 gcaagcccct gtggctctac cagcgcaccg ccacgcccca ggtggatctg gacgacccgg
    38101 agttcgacgc caaacgcgcc cagtaccacg ccgtcaaagc cttcatcgcc gacttccgcg
    38161 cgccagacgg cagcctctcc gccggcatca acacccacgc cgaccctgcc gccttcacga
    38221 tcctgttccg ccagcacctg gaggcccacc tgggcgggct cctcttcgac gcgcctcccg
    38281 tcgccccgga ttccccccgc atcgaccaca tcctgctcgc cctcgacgcc cagctcgccg
    38341 ccaaagactc cacgatcgcc gagcgggacg gcgaaatcgc ccgtctgaag gccgaactcg
    38401 acgccctgcg ccgcagcgcc atcgccgaca ccctcacccg cgccgccgcc tccgacgcca
    38461 cccccgcaga acaggctgcc gcccaggccc tcgaagccgg cgacagcgcc cccgccgaag
    38521 ccctgctgcg cgccgacgaa accgccctca cccctgccga ccgcccccct cccccgccga
    38581 acgccgcgcc gccgcggacc tcgcccgcca gcagggcgcc ctcctcctcg gccacgacgc
    38641 ccagggcgcc ctcgccgcct accagcgcgc ggccgaacac gatccggaag acgtctggac
    38701 ggggttccac atcggcgacc tccacgtcac cctgggcaat ctcgcggcgg cccaggcggc
    38761 ctaccagagc gcccaagccc acgtcaccca ccgcct
//
LOCUS       DAOQMM010000022        37583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_270_length_37583_cov_48.559502, whole genome shotgun sequence.
ACCESSION   DAOQMM010000022 DAOQMM010000000
VERSION     DAOQMM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 37583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37583
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_37583_cov_48.559502"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1117)
                     /locus_tag="PLH85_05785"
     CDS             complement(149..1117)
                     /locus_tag="PLH85_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB64104.1"
                     /translation="MSLSQLYAPIADDMKALDAVIRERLYSDVVLIRQVAEYIIGSGG
                     KRLRPALVLFSAGAMGYRGRFHLELAAVVEFIHTATLLHDDVVDESDLRRGNKTANAL
                     FGNAASVLVGDFLYSRAFQMMVGVDDMRVMRVLADATNTIAEGEVLQLLNCHNADVDV
                     EDYLRVIRYKTAKLFEAATRLGGILGKADPQLEEQLAAFGMHLGTAFQLVDDVLDYSG
                     DEAATGKHLGDDLAEGKPTLPLIHAMRHGSPEQAARVRAAIENGGRDDFAGVLDAVRA
                     TGALEATLAVATQEAERALAMLEGLPPSIFKDSLVHLSKFAVAREY"
     gene            complement(1197..1925)
                     /locus_tag="PLH85_05790"
     CDS             complement(1197..1925)
                     /locus_tag="PLH85_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNB64105.1"
                     /translation="MVAHRCGGALAPENSLAGLEIAARLGCSAVEFDVMLSRDAVPVL
                     MHDETLERTAGLPGRVPDLEGGELAAVDIGRLHHPAFAGECIPRLDTALERCRWLGLA
                     ANVEIKPADDLDEVTARAVIAVVTQCAERDRPAILFSSFSRKALEQVALALPAIPRAL
                     LAERYDADILACARALGCGAVHLEATSLRASDVAAVHAAGLWLRAYTVNQVDLACRLV
                     EWGVDGLFSDRPDRLLRLDALSAG"
     gene            complement(1955..3262)
                     /locus_tag="PLH85_05795"
     CDS             complement(1955..3262)
                     /locus_tag="PLH85_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNB64106.1"
                     /translation="MKNVCRVAAGGAVLGLALLSPVYAAPAKKVAPAVPQATAPVGDL
                     ELVHTLGPDKGAQLQKLVDRFNAANPGTHIVVSERPWTEGALPHMLILGERDLPAFLA
                     GPQRYRPLYQVMKDAGERVETLKAPAFMTPTPLDASNRLLALPVGLSTPVMFYNRDAF
                     KAAGVNPDEPPKTWQDLQGALGKLVANGSTCPYTSAQPAWILVENSSAWHNEPVVAEG
                     KRASLAINGMLPVKHVAMLSSWFKARYLHVFGRGDEATEKFAQGECAVLTAPSAALPT
                     LRREAKFDVGVATLPYHAEYYGAPQNTLADGPALWIGAGKKPAEYKAIARFVNLWLTP
                     ESQVEWQRNAGYLPLNRAGLLAATESRVLGPELEAIRIGVKQLTHKPVTKISSASSYP
                     QRTAVRQIVEEELEAVWADRKPSKVAMDDAVARSRSLGCSPSC"
     gene            3412..3867
                     /locus_tag="PLH85_05800"
     CDS             3412..3867
                     /locus_tag="PLH85_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64107.1"
                     /translation="MKITSVRLALMLALGPTLLGGCERLGIPDPAKAQAQAEAEGKAI
                     GSACRHAGRAIEDCYTLNQAAPKAAIFSGWKEMNDYMVENKIAEVAPQLPPPPPPAPP
                     QKTGKKAKDGKGDKDEDHGDKEDAADSDKSSGGHRADEETPRRRRRSAE"
     gene            complement(3898..5685)
                     /locus_tag="PLH85_05805"
     CDS             complement(3898..5685)
                     /locus_tag="PLH85_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNB64108.1"
                     /translation="MNSIVLRERNGIAVIGNEALARMLARYGFGQAQQAAIRAFGRIV
                     DGQGLTESLLERLDLLSPDGRGEGAIGPALRLFCEDLACLPQWDFTPRWPERLVASWS
                     ECYLAGAVAEFPFVAIQQLVDLCQQRLFDEREMVYRLEMDIFSAVIRLCWCLGGLLSE
                     VSIEREQAFRLYAEDGDPVLGIPNRRRFLALLDQQLAGPDPVGLVVIGVDWGRSEALL
                     PMDERDGIRLAFTEALQGTVRPADILCALGEKEWAVILPGIHHPAQVSLAGHKMVDAC
                     EILRNNSFVSLNGRFAAGGALAPEHATDPLGLEQAARAALVAARHANGYFDLYSASLA
                     RASRADADMEDEVVRAFEACQFDLYLQPQICLTDAQVIGAEALLRWPRGEGVVVGPQR
                     ILAVLGNVGMIQALSRWVIQRAVQAVAALRAAGCQIRVSVNLSAEDLRDQELAVFVRQ
                     TCETWRVPAERLCFEITEGGFVADEAMTARTLESLRETGGWLSLDDFGTGYASMDYLR
                     RMPVQELKLDKSFVERLNRDEADRAIVELMIRIAHTFGLEVVGEGVEDADTALTLAAL
                     GCDHAQGYHYARPMPVDDFIDWWQTREGG"
     gene            complement(5682..7211)
                     /locus_tag="PLH85_05810"
     CDS             complement(5682..7211)
                     /locus_tag="PLH85_05810"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNB64109.1"
                     /translation="MKEIVAVQDLAVGSFICELDRPWLESPFLIQGFLVADETILAQV
                     RSLCRFVYVDRARSVGVEWRPDTVTPAGDADVRAARLAEFHVPAADARRRPQDFFALL
                     RAMRERKADDANADERTREPFVYRYPDRPERARSGSVAEPSADASPAGRVSRPIVTPT
                     GDGKRTGARRAAGGEEGASPSGAAANAGLVYGGPAVEEEIVAAAPLVDEAQVILKEIA
                     RDVQSNLNPDLERLRAVVGEMVLSVARNPDALLWLVRLKQTDQYSYDHSLDVAAHVMI
                     YARHLALPEDVIATLGIAGMLQDVGKLRMPVRLLQKVGAITRTEYEIFKTHVDFSVQI
                     LAANPNASPLLLEIVGQHHERCDGSGYPRGLKDEQISLAAQIAGLCDVYCAMTRNRPY
                     GKAASSQAALDELRAMRGRGFAETTVDQFVQCIGIYPVGTLVELNSGEVAVVIAQNRV
                     RRLKPRVMLLLGPDKKPNAYPLVVDLLYDPPNLAGDPYAIVRSLPPGAYGVDPSEYYL
                     A"
     gene            complement(7257..8294)
                     /locus_tag="PLH85_05815"
     CDS             complement(7257..8294)
                     /locus_tag="PLH85_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB64110.1"
                     /translation="MEVRQLGGSDLRVSAVCLGTMTFGQQNSEAESHALLDLAMARGV
                     NFIDTAEMYPVPPRAETCGRSESIIGTWLRGRPREQVVIATKVAGPARSMDWIRGGPL
                     SLDRDNIRVAVEGSLRRLQTDYIDLYQIHWPARNQPMFGQWRFDAAAEREATPIREQL
                     EAMAELVREGKIRYVGLSNEHPWGVMEFLRHARELGLPRVVSVQNAYNLLNRNFEYTL
                     AETCFREQIGLMPYSVLAFGHLTGKYLVDPPAQGRLSLFPAFGQRYGKANVQPAVQAY
                     AALAQEAGLSPATLAQAFVAGRPFVASTIVGATSPAQLEENLAACARVLEPAVVAQVD
                     ALHLQFTNPAP"
     gene            8451..9539
                     /gene="rlmM"
                     /locus_tag="PLH85_05820"
     CDS             8451..9539
                     /gene="rlmM"
                     /locus_tag="PLH85_05820"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HNB64111.1"
                     /translation="MSTPSPHCLTLGALIQCRPGFEPDAAAELASRAHAARLVGPVSG
                     APRSGFCTLTLAEPLPRDLLQRRLDASQLVFSRQSFQVVAVLDDLPERDRLTPLVSAI
                     AAGGERFCRLLLETPDTNEGKETSGFLRRFEPLLDAALRDAGLLRPDSPHLPRLHVFF
                     ANARHVWLGFSAPSDGSAWPMGIPRLRMPKDAPSRSTLKLAEAFLTLLTPDEQKATLR
                     PGLQAVDLGAAPGGWSFQLASHGLRVTALDNGPLAPAALATGLIEHLRADGFTWKPRK
                     PVEWMVCDMVEQPARIAPLMAEWLASGRCRRSLFNLKLPMKRRYEELERCRALIDQRL
                     RHAGIKYVLRIKHLYHDREEVTCYLTRR"
     gene            9615..11066
                     /locus_tag="PLH85_05825"
     CDS             9615..11066
                     /locus_tag="PLH85_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB64112.1"
                     /translation="MSFSELGLIPEILQAVADAGYTEPTPIQRQAIPVVLAGHDVMGG
                     AQTGTGKTAGFTLPLLNRLARHASASASPARHPVRALILAPTRELAMQVYESVRMYSK
                     HLPLRSVCIYGGVDMNPQIQELRRGIEIVVATPGRLLDHVQQKTIVLNQVEFLVLDEA
                     DRMLDMGFIPDIRRILALLPAQRQSLLFSATFSDEIKKLADQMLKNPQLVEVARRNMV
                     SETITHVVHPVSSGLKRNLLAHLIRHEEEAQVLVFVDTKFGCSRLAHFLERHGISADA
                     IHGDKSQQARTETLENFKSGKTRVLVATDVAARGLDIEDLPSVINFELPHTAEDYVHR
                     IGRTGRAGKTGKAVSLVSAEERHRLADIQKLIKVEIRQEVVAGYDPEPDFFEADGKRR
                     RHAASAPKAEAGGEPRERHERRERRPERSPRESSGRGGPRTPHIAPDGFDFSKPYEQR
                     ETPPHTLADAADASPKKARKPIAFLLGGLGRRD"
     gene            complement(11163..11393)
                     /locus_tag="PLH85_05830"
     CDS             complement(11163..11393)
                     /locus_tag="PLH85_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNB64113.1"
                     /translation="MVFDKEVDARGLNCPLPILRAKKALAELQAGQVLRVLATDPGSA
                     KDFAAFARQTGHELIEQNERPDQSFEFFLRRK"
     gene            11761..12114
                     /locus_tag="PLH85_05835"
     CDS             11761..12114
                     /locus_tag="PLH85_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNB64114.1"
                     /translation="MLQTVLSDLNGTSADIEASAIISSDGLMMAALLPNTVDEDRVGA
                     MSAAMLSLGDRTARELARGNLEQVLIKGEKGYVLMLQAGADAVLTVMAKPSAKLGLVF
                     LDVKRAADSIARILG"
     gene            12134..12607
                     /locus_tag="PLH85_05840"
     CDS             12134..12607
                     /locus_tag="PLH85_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNB64115.1"
                     /translation="MTDMDPASIVGNARECTLQHFQSVARKVYVTDVETPYPQGKLIV
                     SRTNVEGVITHCNQSFVDMSGYEEHELVGQPHCILRHPDMPAAAFRDLWETVQAGRQW
                     HGYVKNLRKDGGYYWVYATVVPNVRDGQIVGYTSVRREPARNRIAEAEALYRSMS"
     gene            12610..13356
                     /locus_tag="PLH85_05845"
     CDS             12610..13356
                     /locus_tag="PLH85_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNB64116.1"
                     /translation="MTLNFTVSPSFPNERISDWYIFNTWLQRALSAQVHLELYDGFGA
                     QRQAIADGKVDLIYANPFDASLLVREKGFRAVAGPDDRADEMVVFTTDASSARQIADL
                     KPGTRLACTDDPAVNTVGRILLEPAGLGPDNTVTVQKETYVQVARDVLQGHVDVGFMP
                     VEAFEGLSRLVRRDLRPLVTSQLHALFHVFLVGPALAARSDEIAELLTGLNDSDKGRQ
                     MLAALGSTHWRRVSQEDAEFMIDIISTLAQ"
     gene            13353..13829
                     /locus_tag="PLH85_05850"
     CDS             13353..13829
                     /locus_tag="PLH85_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64117.1"
                     /translation="MSLFITDTAQPGRIRPPSVPAVFGLPVRTLNIASHWHSPATGAL
                     LAAEIGRTPANLHAHVQRISLWSSLGDSRELIAAIIDLWMVLGPHGMDLRKRMLDTHR
                     EILEREGLAAWMSAQLSTGADRHDPLLARPGVVLARPVRGIRRFLRTPATLSAART"
     gene            13925..15106
                     /locus_tag="PLH85_05855"
     CDS             13925..15106
                     /locus_tag="PLH85_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64118.1"
                     /translation="MKHPPRPSARSQPLALHLLEVGSMDQRLIGLSVERHMKHVLRLV
                     TEPDQARAVLIDCDRQGAQDALRQARLLRGVGIVGYAFNPRERNNAFPGIEILNKPLA
                     LDRLPEALDRAIAAAGAAPPTIPADSPPPAAPPAPPTLGEGVQADDADLCGALEDLPP
                     APGAPLPDKVFFDPSDYLIGTLAQAVRTTAELGRPQAIAGLPRIIRIEPKPAPTCIAG
                     YRDNQLRPLSMAQLPQSTARIVPAPSFREHIAGEVACAAEELLWNVAAWGARGRIPAG
                     HDPYRPVRLRAWPNFPAIFVSPHALRIAALWVRSHATPVEIASRLAIPQRYVFSFYSA
                     AHFSGLLDLQPPPPTPAPVESAPQTERVPDTPVASRHPAPSPQKPSLLSRILRKLLNA
                     V"
     gene            15096..15656
                     /locus_tag="PLH85_05860"
     CDS             15096..15656
                     /locus_tag="PLH85_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNB64119.1"
                     /translation="MRSERARHNKIVFSGTVGAGKSTAIAALSDIPPVSTEADATDET
                     ADIKKTTTVAMDYGLLSLPDGEKVMLYGTPGQERFSFMWEILSEGGIGLILLINNDTP
                     APLDDLERYLKAFKNFIASTAVAIGVTHMDKAPVPSLDTYRAKLAEVGLHPMPAVFRV
                     DARSREDVATLVKALLFTLNPGLIDA"
     gene            15649..16281
                     /locus_tag="PLH85_05865"
     CDS             15649..16281
                     /locus_tag="PLH85_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64120.1"
                     /translation="MLEIIPGSYLAVTPYGAWRAVSRADDSIERRILQAILCEAQSPM
                     LSAEAACAWTGESNAEAALGHLLRLQEDASLEAWTTPRKVPFGSMEASLPPLLATLSD
                     TRRAVLADRMGFCLAMAGFPQEQGDALAALGADILSVAERQSAALAQIGDQLLDGWGN
                     VDAAGVSGLSFWPMHVGRIRLALTVSGRPRFNQATFSDLVWSLCVRYGRI"
     gene            17480..17875
                     /locus_tag="PLH85_05870"
     CDS             17480..17875
                     /locus_tag="PLH85_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNB64121.1"
                     /translation="MSQLEDLKSELNKIDGEIAALSQRRIDLQTQIDAEQKKYAEEAL
                     SKIVGELKSLNLDPADIAKALGVPVTPTAKKTRAPRGSAPVKSKGEPKYRNSVDPSKT
                     WSGKGRKPEWVITYLANGGKLEDLLIHKA"
     gene            18067..18546
                     /gene="moaC"
                     /locus_tag="PLH85_05875"
     CDS             18067..18546
                     /gene="moaC"
                     /locus_tag="PLH85_05875"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNB64122.1"
                     /translation="MSDPFTHFDAQGQAHMVDVADKAETRRVARAEGCIVMRPDTLAR
                     ILAGDNRKGDVIGIARIAAIQGAKRTADLIPLCHPIPLTRVSADFTVEGADTIRCTVT
                     AETVGRTGVEMEALTAVQIGLLTIYDMCKAVDRGMLMQGIRLLEKEGGKSGHWVAEA"
     gene            18853..20358
                     /locus_tag="PLH85_05880"
     CDS             18853..20358
                     /locus_tag="PLH85_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNB64123.1"
                     /translation="MSHEIELKLTLPKKALAALRKHPLLAEAPREGRTCTLENTYYDT
                     PELELRERRMAVRTRKAGSRWLQTVKCAAESVGGLSARPEWEQPYVNASFDFSTVDRD
                     DVRERLETYHPRLVPVFTTQFRRETVVLTPRPGTRVLAMIDSGTIEAGERSAALCELE
                     LELVDGDPRDLYAIALQLAETLPLTPENVSKAERGYRLYSNLALRPAKARPSRLSAEL
                     SPVDAFRLIAFDCLALWQANESGARESDDPEFIHQLRVALRRLRSALRLFGPALPDDF
                     TRRWSDGLKEAADGLGAARDLDVMLAEVLSPVAESGLADEATQRLIAMAVDRRDREKT
                     AARAQLLAASHGRQMLALAADLYALESDSLVKSAQLSTFARLQLSQLRKRARKRYSAA
                     GDLQAEKLHELRVALKRLRYGIEFLLPLLPQKATVRYANALADAQETLGYLNDEAVAR
                     RRLRDWAGKDPELRSAAAFVAGWHAPRIARLRRNVLPGLEALLWGKAPWSR"
     gene            20431..20883
                     /locus_tag="PLH85_05885"
     CDS             20431..20883
                     /locus_tag="PLH85_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNB64124.1"
                     /translation="MDLLLWRHAEAVDGVPDSTRELSERGLRQAHKMAEWLDKRRPKR
                     LRVLVSPTLRTRQTASAFTHDFEVVPALGPGNDIASILAATGWPDAGGAVLIVGHQPE
                     LGRLAALLLAGQEADWTIKKGALWWFTNRVRDEETQTVLRAVVPADLA"
     gene            complement(20943..21389)
                     /locus_tag="PLH85_05890"
     CDS             complement(20943..21389)
                     /locus_tag="PLH85_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNB64125.1"
                     /translation="MSDQSKQFEEFYGYTFEELSVGQSAVYARTVTEADILMFAGVSG
                     DYNPVHINQELAESTMFGGRIAHGMLSAAFISTVFGMKLPGPGAIYLSQSLKFKAPVK
                     IGDTVVTRVTIKELVPEKRKAKFDTVCSVRGKNVVEGEAEIMVPAR"
     gene            21583..22431
                     /locus_tag="PLH85_05895"
     CDS             21583..22431
                     /locus_tag="PLH85_05895"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB64126.1"
                     /translation="MSQPDQAVLDRLLRGIDIPPCPAVLTALQREVAAPNASLTRIAQ
                     IINGDVSLAASVLKAANSPAFGLSRRVGNVAQAVPVLGMRTLSAITAGVALRTALSGG
                     ARRLERFWDSSSKTALLCARLTREFTGVPAETAYTFGLFHDCGIPVLIHRFPDYLDTL
                     QLANGSFDLSFTEVEERRHATHHAAIGTLLTRNWGLPEDISTAILLHHDLDIFRPDAD
                     HIAARARTLVALGALASYFVGSSLRMSEDPWWIRGRPLYLAHLGLGDKDFLDLRETAF
                     EAIESF"
     gene            22445..23947
                     /locus_tag="PLH85_05900"
     CDS             22445..23947
                     /locus_tag="PLH85_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNB64127.1"
                     /translation="MPAWPLYPVSALRAVEALALPGARPPLMERAGAAAADLARRLLG
                     SSAGDVLVLCGPGNNGGDGFVLARHLLQAGHRVCVVCTADPARLPADAAAARQTWLDA
                     GGTTQTHFPAAPASGWALAVDALFGIGLQRPPTGQYAAWIEAFNALDCPRLALDIPSG
                     LDADSGRRLEVCVDATHTVTFIAAKPGVCTLDGPDTCGVLEIAPLGLDPEALVPAPGR
                     TISPALFAESLRLRRRNTHKGSFGDAALIGGAPGMVGAALLAGRAAAFLGAGRVLVGL
                     LAADALAVDPLRPELMFRNAHGLLAENAGAYAIGPGLGQSDAARSITADALRLQRPLV
                     LDADALNLVARHAELQHALTARDAPTLLTPHPAEAARLLACSTADIQTDRIAQALALA
                     HRFNALVVLKGCGSVVAAPDGAWWINTSGNPAMATAGMGDVLTGLIVALLAQGWPARS
                     ALLAGVHLHGCAADLCCAEQGLHAGLLAGEILEPARRCLNRWLGAPEARP"
     gene            23944..25926
                     /locus_tag="PLH85_05905"
     CDS             23944..25926
                     /locus_tag="PLH85_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB64128.1"
                     /translation="MNGTAFDPAGRSIRSIRLFPLPPVDATMPLTDALRVLLDQNVPA
                     ALVRLQGTVIGILSERSAVGACLGADASRLTARDACLLNPPVCGPDTELREAYRLLVR
                     HDSRDLLVCEADGRPTGLVAESDILSALGMEHYAHLDQVGQVMSLRPRSVAETASTED
                     ALRVMRRHDIGSVVVVTDQWPVGIVTFHDLSPRVARGERLHELPVTDVMVRDPITIAP
                     HTSVWEASQIMHRARIRHLPVVHHDRLVGMLAEHDIVTSLEGRYVAFLREIIVEQERE
                     LIAQQVHIARRESEESLRRIIDASPDMVIVKDPQGCWQIANRAALELFGLDHDACHGR
                     AEEAFADRVPQPRREALLRYAAASRHAWQSPDGLRHSDRVPAAEGADVHVEFIEKPMF
                     GADGAPSMLVTLGRDITDRVRAEDAVRALNASLEERVAQRTRELRATLAELETFNYSI
                     SHDLSTPLRAIDGFSGIVMQDYADQLDPQALEYLRRVRDASRRMARMIDDLLDLSRHS
                     RKPLERSPLDLSAMARSVADELVHGDPGRQAHIHVEPGIEAEGDPGLLRTVLQNLLGN
                     AWKFTRPQACADIRFSMRRRQGERIFTVSDNGVGFDAAHAAHLFVPFQRYHAEEDFEG
                     SGIGLSTVQRIISRHGGRIWAESAPGQGCSFHFTLG"
     gene            25930..26802
                     /locus_tag="PLH85_05910"
     CDS             25930..26802
                     /locus_tag="PLH85_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB64129.1"
                     /translation="MHAPPRPARGVLFAMVSLVLFVSLDVTSKYLSQRYPVPLLIWAR
                     YTVHCLLMVLVLGPSQRGRLLATGRPALHVLRAGLLLATSLCGLSAFQRLPLAEATAI
                     AFCSPLIVTLLARPLLGERVGPARWVAVLVGFAGVMLVARPGSGLVPDGVLFAFATAV
                     VYALYQLLTRKMAATERPLTLLFYSALVGSAVMSATLPWLDRGPTPSLPELPLFLAMG
                     TLAGLGHFCLTRAFREAPASLLSPLLYGQLFWATLFGWAVFGQFPDRLALTGMLAIGA
                     SGVLIAVDSRRGMR"
     gene            complement(26812..27648)
                     /locus_tag="PLH85_05915"
     CDS             complement(26812..27648)
                     /locus_tag="PLH85_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB64130.1"
                     /translation="MHAFRFRAGLCCIVGALCLGRAVSAEAALLDEDELAEVVRKPTI
                     GLYKGYAEFKMAHYAEARQIWTALADRGQGEAWFNLGILAEDGLGEPRDGTEARRRYE
                     RAAQAGSRNAALRLGTLLQTARLGAPDPAAARRWLQQAADLGDDEARAQLAALEGRAV
                     AVNGADRRLAEARRLEADGKLADAVDAYRAIAAAGDVRGTTRLAWLFEAGRGVPRDLA
                     EAARLFRQAAEAGEAEAQYALAVMLETGAGQPRDPEEALRWLRASAAGRYPPAVDALK
                     AR"
     gene            27874..28722
                     /gene="nadC"
                     /locus_tag="PLH85_05920"
     CDS             27874..28722
                     /gene="nadC"
                     /locus_tag="PLH85_05920"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNB64131.1"
                     /translation="MEFADQLRVEVQRSVAASLAEDIGTGDLTARLIPAAREARGRVI
                     TREDAVLCGTAWFDAAFAALEPSASVIWHARDGERVKPGQLLCEVDASARALLTAERT
                     ALNFVQLLSGTATLTATFVEAVAGTRARIVDTRKTLPGLRLAQKYAVKVGGGTNHRIG
                     LYDGILIKENHIIAAGSIRAVIEQARAIAPSNVFIEVEVETLDQLREALAAGARMILL
                     DNMDLDQMREAVRLNEGRAELEASGGVNLQRVRAIAETGVDRISIGSLTKDVRAIDLS
                     LRHIEE"
     gene            28778..29674
                     /locus_tag="PLH85_05925"
     CDS             28778..29674
                     /locus_tag="PLH85_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64132.1"
                     /translation="MRIENDINVAPSESPTVADAVEKLVLRITARDTGIDLSAVTRIL
                     ITADLPDATQRFGGTPVTPDQLTRIVREGQGYMLLFSGVRMLEMFKGGVAEVSRVIHH
                     LHKDLWHLHDVARPRTPKPTTPLARALAPICAIMWQEYRVNRRTAWSVPRDSDLLMHH
                     LAALLQALPGGMQEELTLYFADQDLDALFAKSLGRISHLMQAVAHVQGYLDGIGQPLQ
                     TISPDMHEVVNQSFLAPLWTTTARHLAAGYEHYPAEVADLPLRRDIQNAFATFGLTIR
                     ENDAGDDVWLQVEPPVPPEKMH"
     gene            complement(29703..30056)
                     /locus_tag="PLH85_05930"
     CDS             complement(29703..30056)
                     /locus_tag="PLH85_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNB64133.1"
                     /translation="MQKILIIVHAPPYGSERCLSALRLAIALAGSDAQPKVELFLMSD
                     ATVMALPHQQDANGNGLQGMVEELVRNGVAIRLCRTCALARGLGDLPLIPGTTIGTLP
                     ELAQATLDADKVITF"
     gene            complement(30133..30339)
                     /locus_tag="PLH85_05935"
     CDS             complement(30133..30339)
                     /locus_tag="PLH85_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNB64134.1"
                     /translation="MESRLTEIEIKLALTEDLVETLNLTVHRQQEQIDALQRQLLALA
                     KQVHSTSASTAEPHSLRDEIPPHY"
     gene            30708..31706
                     /locus_tag="PLH85_05940"
     CDS             30708..31706
                     /locus_tag="PLH85_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNB64135.1"
                     /translation="MESIGTWWMWAGFFALVLVMLAIDLFAVGGGRRHRVSLREAGLW
                     SAIWVGVTLAFAGALWVWLDGSAGRETATTRTLEFLTGYLIEKSLAVDNVFVWLMLFS
                     FFGIPLELQKRVLTYGVLGALVLRTLMILGGAWLIVQFHWLLYVFGAFLLLTGVKMLW
                     LADARPDLAANPVLRWIRRHVPVTEALHGERFIVTLPGTNGPVRALTPLALALVMVEL
                     SDVIFAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLIAGMADRFALLKYGLALV
                     LVFIGAKMLLLDVVKIPIALSLTVVALLIGGAVMLSLHRERRRALPEPRRDDGGGR"
     gene            31766..33700
                     /locus_tag="PLH85_05945"
     CDS             31766..33700
                     /locus_tag="PLH85_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNB64136.1"
                     /translation="MIPTRQHLELLAPAKNADFGIEAINHGADAVYIGGPAFGARAQA
                     GNEVADIERLARHAHRYGARVLVALNTILHDAELDGAEALIRQVYEAGADALIVQDMG
                     LLELDLPPIQLHASTQTDIRHPEKARFLQDVGFSQIVLAREMSLTEIRKVAAVTDCAL
                     EFFVHGALCVAFSGQCYISHAHTGRSANRGECSQACRLPYDLEDQDGHLVTGNRHLLS
                     MKDNDQSANLRALADAGISSFKIEGRYKDIGYVKNVTAHYRTLLDQLLEDAPDYRRSS
                     SGRCTFLFTPQPEKTFNRGATDYFVNGRQGDIGAFDSPKHVGEPVGSILRIDPQRRSF
                     ELSSAVELHNGDGLSFYDRKGELIGLRINRAEKLGDDFRVHTADAVPADLRPGSGVFR
                     NHDHAFERLMDKKTAERRIAVDLHLTDDTDGLVLQVTDEDGFSAQARLPHPLEAAQNA
                     ERARSALHDGLAKLGATIFSARSIRLDLATPWFVPAAALNGLRRDAIERLEAARLAGL
                     PRPPRRAAVEPPAPYPQTELSYLANVLNERARSFYRRHGVTLIEDAYEANQVLDDASL
                     MITKHCLRYSFNLCPKEVKGIRPDPMTLVNGKERLTLRFDCKRCEMHVIGRLRPAVLD
                     AVPQPVRFHAQAPAALRAGR"
     gene            33725..34435
                     /locus_tag="PLH85_05950"
     CDS             33725..34435
                     /locus_tag="PLH85_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64137.1"
                     /translation="MKLAITLIVLALLIIVGPAAWQFTHTNPAAVPTSGLPWQIDTPA
                     PGESRVFNLTLGRSTLADARTRFGPDAQLAIVATPSEAGNVELYYESITAGFIAGRLI
                     VTADLPAATVEGLRERAAKTEYMQSTTRKATLAEADLPVALAAPIRAIAFIPSANLDE
                     AIVVERFGAPAERLRSNDHIEHLLYPAKGLDVVLDSKGKELLQYVAPARFDALRTPLR
                     AAADQAATAPPAAAPDKP"
     gene            34486..34803
                     /locus_tag="PLH85_05955"
     CDS             34486..34803
                     /locus_tag="PLH85_05955"
                     /inference="COORDINATES: protein motif:HMM:NF039672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HNB64138.1"
                     /translation="MSTPSTVRHFVTYSGVSLPLKLTTPLEGADLAHRITFYRAHYDA
                     AERLALVEKVVYGEIESTHHYTYYPSGVLQEARVCQIADEETTLMAYDETGALVSTTR
                     LED"
     gene            34815..35075
                     /locus_tag="PLH85_05960"
     CDS             34815..35075
                     /locus_tag="PLH85_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64139.1"
                     /translation="MAVQSVQRRRCLTCNRWGGQRRPGDEPDTVEFDEAHDRGPCQDG
                     PWHGTLRGPRNACGQWLRWTLIVPAPDPAEAPAQASPPHEQP"
     gene            35062..35454
                     /locus_tag="PLH85_05965"
     CDS             35062..35454
                     /locus_tag="PLH85_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB64140.1"
                     /translation="MNSPDDSDLLVACLCAAWCGTCRDYAAGFHALAERFPGARFLWL
                     DVEDEADLLDDYDVENFPTLLIQRGDAVLFFGTMLPHHELLERTVRAFASMTPDESRH
                     YAQADATRRTWQHDRNLRRALAERDDDA"
     gene            35479..35889
                     /locus_tag="PLH85_05970"
     CDS             35479..35889
                     /locus_tag="PLH85_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNB64141.1"
                     /translation="MDTCVFCRIVRGELPASVIHEDEHTLAFMNIMAGNPGHALVIVK
                     PHVENIYTLDDALAAAVFRTTTRVAKAVRAAAGCEGVSLFQANEVAGGQTVFHFHIHV
                     LPRLPGDALPGVWPAVAPSREALDAMAARLRAAL"
     gene            36024..37544
                     /locus_tag="PLH85_05975"
     CDS             36024..37544
                     /locus_tag="PLH85_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB64142.1"
                     /translation="MHIPLLSAEAPITLILMVIAGLIMLRPLAHLRRSLMNTGVFYGA
                     CFAGDLLAGLLEGSSQPAAARWLRELCVFGEGLAVVRFVGLTLFQVILPRLHVNISGI
                     LADILVIVGYILWGFIRLSMSGVEFTALFTTSAVLTAVVAISMQETLGNLLGGLALQM
                     DNSLEIGDWIQMGDLAGRVTDIQWRYTAIVTRAGERMVVPNSHLMKNRYAIVCDKRQS
                     NPHQRRHIPFNVDLSVPPARVIGAVLQDILTARIPNVSATPAPACLLMDFGPGFARYQ
                     LRYWLDDPGPDEITDSDVRSHILAALQREDIRLAVADQTVHLVQEGKAHRAEVQAREL
                     NRRLQAITHIDLFCKLSEAEQLELARRLVNAPFARGDIVTRQGAVAHWLYIIVSGEAE
                     AWWQPPDGSPQRLLEKRGPGTVFGELGLMTGAPRRATVIATTDVEAYRLDKTGFEHII
                     RARPEIAEGISAILERRLSHFQALERGDGAPRPDGNKPANGDTGVIGRKIREFFGL"
BASE COUNT         6376 a        14405 c        11072 g         5730 t
ORIGIN      
        1 ccggcccctg cgtcccgaac gcagtgctct accaggctga gctattcccc gacacgatcg
       61 aaagatggcg aaaacacaaa tgccaccttt ctgaaacaaa acagccgcac tgactatcga
      121 cgattcggac tgtgctgcac gccgaacatc aatattcccg cgcgactgcg aactttgaca
      181 agtgtaccag cgaatccttg aaaatggaag ggggcagccc ttccagcatc gccagcgcgc
      241 gctcggcctc ctgggttgcg accgcaagcg tagcctccag tgcgccggtg gcacgcacag
      301 cgtccagcac gccggcaaag tcgtcacggc ccccgttctc aatggcggcc cgcaccctcg
      361 ccgcctgctc gggtgaacca tgccgcatgg catgaatcag gggcagggtc ggcttgccct
      421 ctgcaagatc gtcacccaga tgcttgcccg tggccgcctc gtcgccggag taatcgagca
      481 catcgtccac gagctgaaag gcggtgccca agtgcatgcc aaaggccgca agctgttctt
      541 ccagttgcgg atcggccttg cccaggatgc cccccaggcg cgtcgctgcc tcgaacagct
      601 tggcggtctt gtagcggatc acccgcaggt agtcctccac gtccacatcg gcgttgtggc
      661 aattgaggag ctgcaagacc tcaccttcgg cgatcgtgtt ggtggcatcg gccagcacgc
      721 gcatgacgcg catgtcgtcc acacccacca tcatctggaa cgcacgcgaa tacaggaaat
      781 ctcccacaag caccgacgca gcgtttccga acaatgcatt ggccgtcttg ttgccccgcc
      841 gcagatccga ttcgtcgacc acgtcgtcat ggagcagcgt ggcggtgtgg atgaattcca
      901 ccacggcggc gagctccaga tggaaacgcc cgcgataccc catggcaccg gccgaaaaga
      961 gcacgagcgc ggggcgcagg cgcttgccgc cgctgccgat gatgtattcg gccacctggc
     1021 gaatcagaac cacgtcggag tagagacgtt cgcggatgac ggcatcgagc gccttcatgt
     1081 cgtcggcgat cggtgcgtaa agctgcgaga gagacaaggc agtgcccaga cgggaaaaag
     1141 cgcgatggta gggggagctc agcccgtgcg tcaagcggcg tggcgtaata ccctgctcag
     1201 cccgctgaca atgcgtccag ccgcagcagg cggtccggtc ggtcactgaa cagaccatcc
     1261 acgccccact ctacgaggcg acacgccaga tcgacctgat tgaccgtgta cgcccgtagc
     1321 cagagccccg ccgcgtgaac ggctgcaaca tcgctggccc gcagtgacgt cgcctcgaga
     1381 tgcacggcac cacagcctaa ggcgcgggcg cacgccagaa tgtcggcgtc gtagcgttcc
     1441 gccaggaggg ctcggggtat cgcagggagc gcaagggcca cctgttccag cgccttgcgc
     1501 gagaaagacg agaaaaggat cgccggtcga tcgcgttccg cgcactgcgt caccacggca
     1561 atcaccgccc gtgcggtcac ctcgtccaaa tcgtcggcgg gtttgatctc cacattcgca
     1621 gccagaccca gccaccggca gcgttcaagc gccgtatcga ggcgcggaat gcactcccct
     1681 gcgaaggcag ggtgatgcag gcgaccgata tcgactgcag cgagctcccc cccttccaga
     1741 tcgggaacac gcccggggag cccggcggta cgctccaggg tttcgtcgtg catgagcacc
     1801 ggcacggcgt cccgtgacag catgacgtcg aactccaccg cggagcaacc gagccgcgcg
     1861 gcaatctcca gcccggcaag ggagttctcg ggcgccagcg caccgccgca gcggtgggcc
     1921 accacccggg gccacgctcc gccgggtccc tccatcaaca ggagggcgag cagccgaggg
     1981 aacgcgaacg ggccacggca tcgtccatgg ccaccttgga gggcttgcgg tcagcccaca
     2041 cggcttccag ttcctcttcg acgatctggc gcacggcggt acgctgggga tagctgctcg
     2101 ccgacgagat cttggtgacc ggcttgtggg tcagctgctt gacaccgatg cgaatggcct
     2161 ccagttcggg cccgagcaca cggctttctg tcgccgccag cagaccggca cggttcagcg
     2221 gcagatagcc cgcgttgcgc tgccattcga cctgactttc aggcgtcagc cacagattga
     2281 cgaaacgggc gatggccttg tattcggccg gcttcttgcc agcaccaatc cacagagccg
     2341 ggccgtccgc cagcgtgttc tggggtgcgc catagtattc ggcgtgatac ggcagcgtgg
     2401 ccacacccac gtcgaacttc gcctcgcgac gcagggtcgg gagggcggcg gacggcgccg
     2461 tgaggacggc gcattcacct tgggcaaact tctcggtggc ttcgtcgccg cgaccgaaga
     2521 cgtgcaggta gcgggccttg aaccaggagg acagcatggc cacatgcttg acgggcagca
     2581 tgccattgat ggccagggaa gcccgcttgc cctccgccac caccggctcg ttgtgccagg
     2641 cgctgctgtt ttccaccagg atccaggcgg gctgagccga ggtgtaggga caggtgctgc
     2701 cgttggcaac aagcttgccg agcgcgcctt gcaggtcctg ccaggtcttg ggcggctcat
     2761 ccggattgac gcccgctgcc ttgaaggcat cacggttgta gaacatcacc ggcgtggaca
     2821 ggccgaccgg cagggccagc agtcgattcg aggcatccag cggggtcggc gtcatgaagg
     2881 ccggcgcctt gagggtttcg acccgttcgc cagcatcctt catgacctgg tagaggggtc
     2941 gatagcgctg cggcccagcc aggaaggcag gcaggtcccg ttcgccaaga atcagcatgt
     3001 ggggcagcgc gccttccgtc cagggccgct cgctgacgac gatatgagta cccggattgg
     3061 cagcgttgaa gcggtcgacg agcttttgca gctgggcacc cttgtcgggt ccgagggtat
     3121 gcacgagttc gagatcgccg acaggcgcgg tggcctgggg cacagccggg gccaccttct
     3181 tggctggcgc cgcgtaaacg ggggacagta gggccaggcc caggacagcg ccgccggcgg
     3241 cgacgcgaca gacattcttc atggagaggg aatccgcagg aaacaaggag agaggttacg
     3301 caccgctaac cggtcattat agggacaagc cgcggcggga agtttaagca aatgttcaag
     3361 tcacctgcag accggccgtt gactggcttg cctcacgatc cctcgacgca gatgaagatc
     3421 acctctgtcc gccttgcact catgctggcc ctcggcccca cgctgctggg ggggtgcgag
     3481 cgcctgggca tccccgatcc ggccaaggcc caggcccagg cggaagccga aggcaaggca
     3541 attggcagcg cctgccgcca tgccgggcgg gccatcgagg actgctacac cctcaaccag
     3601 gcggccccca aggcggcaat cttctccggc tggaaagaga tgaacgacta catggtcgag
     3661 aacaagatcg ctgaagtcgc gccccagctg cctccgccgc ccccgccggc gcccccgcaa
     3721 aaaaccggca aaaaagcaaa ggacggcaag ggcgacaagg acgaggacca cggcgacaag
     3781 gaggacgccg ccgattcgga caagagcagc ggcgggcatc gcgccgacga agaaacgccc
     3841 cgcaggcgcc gacgcagcgc cgaatagcac cgcagttccg tccgggcatc gcccggttca
     3901 acccccttcg cgcgtctgcc accagtcgat gaaatcgtcg accggcatcg gccgtgcata
     3961 gtggtacccc tgcgcgtgat cgcaccccag cgccgccagt gtcagcgcgg tgtcagcatc
     4021 ctcgaccccc tcgcccacta cttcgaggcc gaaggtatgg gcgatgcgga tcatcagttc
     4081 gacaatggcc cgatcggctt cgtcccggtt gagtcgctcc acaaaggact tgtccagctt
     4141 gagctcctgg accggcatac ggcgcaggta atccatcgag gcgtagccgg tgccgaaatc
     4201 atcgagcgac aaccagccgc cggtttcgcg caggctctcc agtgtccgtg ccgtcatcgc
     4261 ctcatcggcc acaaagccac cctcggtgat ctcgaagcac aggcgctccg ccggcacgcg
     4321 ccaggtctcg caggtctgcc gcacaaagac ggccaactcc tgatcgcgca ggtcctctgc
     4381 ggacagattc accgacacgc ggatctggca tcccgccgcg cgcagcgcag cgaccgcctg
     4441 caccgcacgc tggatcaccc agcgcgacag ggcctggatc atccccacat tgccgagcac
     4501 tgccagaata cgttgcggcc cgaccacgac cccctctccc cgcggccagc gcaacagggc
     4561 ttccgcgccg atcacctgtg cgtccgtcag gcagatctgg ggctgcagat acaggtcgaa
     4621 ctggcaggct tcaaacgcac ggacgacctc gtcttccatg tcggcatcgg cccgcgaagc
     4681 ccgcgcgagg gacgccgaat agaggtcgaa atagccattc gcatgacggg ccgccaccag
     4741 cgcggcgcgg gccgcctgct ccagcccgag cgggtcggtc gcatgttccg gcgccagcgc
     4801 cccgccagcg gcaaagcgtc cattcaggga gacgaagctg ttgttgcgga ggatttcaca
     4861 ggcatccacc atcttgtgtc cggccaggga gacctgcgcc ggatgatgga tgcccggcag
     4921 gatcacggcc cattccttct caccgagcgc gcacagaatg tcggcgggcc gcacggtccc
     4981 ctgcagggcc tcggtaaacg ccagacggat accgtcgcgt tcgtccatgg gcagcagggc
     5041 ctccgaacgt ccccaatcga cgccgatcac caccagacca acgggatcgg gcccggcgag
     5101 ctgctgatcg aggagcgcca ggaaacgacg ccgattggga ataccgagaa ccggatcgcc
     5161 atcctccgcg tacaggcgga aggcctgctc ccgctcgatg gacacctccg acagcaatcc
     5221 ccccaggcac cagcacaggc ggatcacggc gctgaagatg tccatctcca gccgatagac
     5281 catctcgcgc tcatcgaaca ggcgctgctg gcacagatcg acgagctgct ggatggcgac
     5341 aaacgggaac tcggcaaccg ctccggccag gtaacactcc gaccagctcg ccaccagccg
     5401 ctccggccag cgcggcgtga aatcccattg gggcagacac gccagatcct cgcagaacag
     5461 gcgcaacgcg ggaccgatgg cgccttcgcc ccggccgtcc ggactcagca ggtcgagacg
     5521 ctccagcaag gactcggtca atccctgccc atccacgatc cgcccaaagg cccggatcgc
     5581 tgcctgctgg gcctgtccaa agccatagcg cgccagcatg cgtgccaggg cctcgttgcc
     5641 gatgacggcg ataccgttgc gctcccgcag tacgatgctg ttcatgccag gtaatactcg
     5701 gaaggatcga ccccataggc gccgggcggc agggatcgca cgatggcgta aggatcaccc
     5761 gccagattgg gcgggtcgta gagcagatcg accaccagcg gataggcatt gggcttcttg
     5821 tccggcccga gcagcagcat cacccggggt ttgagccggc gcacgcggtt ctgggcgatc
     5881 accaccgcca cctcgccgga gttcagctcc accaacgtcc cgaccgggta gatgccgatg
     5941 cactggacga actggtcgac ggtggtctcg gcaaaccccc gcccacgcat ggcgcgaagc
     6001 tcgtccaggg cggcctggga gctggccgcc ttgccgtagg gccggttccg cgtcatggcg
     6061 cagtagacgt cgcacaggcc cgcgatctgg gccgcgagac tgatctgctc gtccttgagc
     6121 ccgcgcggat agcccgaccc gtcgcagcgc tcgtggtgct gcccgacaat ctccagaagc
     6181 agcggcgacg cgttcgggtt ggccgccagg atctggaccg aaaaatccac gtgggttttg
     6241 aagatctcgt actcggtgcg ggtgatcgca cccactttct gcagcaggcg caccggcatg
     6301 cgcagcttgc ccacgtcctg gagcatgccc gcaatgccga gcgtggcgat gacgtcctcc
     6361 ggcagggcca gatggcgcgc gtagatcatc acgtgcgctg ccacgtcgag ggagtggtcg
     6421 tagctgtact gatcggtctg cttgagccgc accaaccaca ggagcgcgtc cggattgcgg
     6481 gccacgctca gcaccatctc gccgacgacg gcccgcaggc gctccagatc ggggttgaga
     6541 ttgctctgca cgtcgcgggc gatctccttg agaatcacct gcgcctcatc caccagcggc
     6601 gcagccgcga cgatctcctc ctccacggcg ggcccgccat agacgaggcc agcgttggcc
     6661 gccgcacccg acggcgacgc gccctcttca cccccggccg cgcgccgggc gccagtacgt
     6721 ttgccatcgc cggtcggcgt cacaatcggt cggcttaccc gaccagcagg gcttgcatcc
     6781 gccgaaggct cggccacgga accgctgcgc gcgcgctcgg gacggtcggg ataacggtaa
     6841 acgaagggct cacgggttcg ctcgtccgcg tttgcgtcgt ccgccttgcg ttcacgcatg
     6901 gcgcgcagca gcgcaaagaa atcctgggga cgccgacggg catccgctgc cggcacatga
     6961 aactctgcca gccgcgcagc gcggacgtcg gcgtcacccg ccggcgtgac cgtgtcggga
     7021 cgccattcca cgcccaccga acgggcccgg tccacataga cgaagcgaca cagggagcgc
     7081 acctgcgcaa ggatcgtttc gtcagcgacg agaaatccct ggatcaggaa cggcgactcc
     7141 agccatggcc ggtcgagttc gcagatgaac gaaccgaccg ccagatcctg aaccgccacg
     7201 atttccttca aacccttctc ccgatgacca caacaagccg cgtcgcaaga gacgtactac
     7261 ggtgccggat tcgtaaactg aagatgcagc gcatccacct gcgccacgac ggccggttcc
     7321 agcacccggg cgcacgcggc cagattctct tccagctggg caggcgaggt cgctccaaca
     7381 atcgtgctgg cgacaaacgg ccgcccggcc acaaaggcct gagccagcgt ggccggtgaa
     7441 agaccggctt cctgcgccag cgccgcgtag gcctgcaccg ccggctggac attggccttg
     7501 ccgtagcgct gaccaaaggc cgggaagagc gacaggcgcc cctgcgcggg cggatccacc
     7561 agatattttc cggtgagatg cccgaaagcc agcaccgaat acggcatcag gccgatctgc
     7621 tcgcgaaagc aggtctctgc cagcgtgtat tcaaaattgc ggttgagcaa gttgtacgca
     7681 ttctggaccg acaccaccct aggcaggccc agttcccgcg catggcgcag gaattccatc
     7741 acgccccagg gatgttcgtt ggaaagcccc acgtagcgga tcttgccctc gcggaccagc
     7801 tcggccatcg cctccagttg ctcgcggatc ggcgttgcct cccgctcggc ggcagcgtcg
     7861 aagcgccact gcccgaacat gggctggttc cgcgccggcc agtggatctg gtagaggtcg
     7921 atgtaatccg tctgcaggcg ccgcaggctg ccttccaccg ccacccggat gttgtcccgg
     7981 tcgagggaca gcggcccgcc gcggatccag tccatgctgc gtgccggacc ggccaccttg
     8041 gtggcgatca ccacctgctc gcgcggacgt ccgcgcagcc aggtgccgat gatggattcc
     8101 gaacggccac aggtttcggc ccgcggcggc accggataca tctcggccgt atcgatgaag
     8161 ttcacgcccc gcgccatcgc cagatccagc agggcgtggc tctcggcctc gctgttctgc
     8221 tggccgaagg tcatggtgcc gaggcagacc gccgacaccc gcagatcgct gccacccagt
     8281 tgtcgtactt ccatgcgctt gctccacaga ccggccgggc ggaagccgga cgcaatcggg
     8341 tatgatcggc accggcctgc gcctgcctcg accgtcgagc cccgcccgcc acagaccaac
     8401 cggcgcattc tacatcccgt cccccgccgc ccggcgaccg attatccccg atgagcaccc
     8461 cgtctccgca ctgcctgacc ctgggcgccc tgatccagtg ccgccccggt ttcgagccgg
     8521 acgccgcggc cgaacttgcg tcccgcgccc atgcagcgcg cctcgtcgga ccggtgtccg
     8581 gcgccccccg cagcggcttc tgcaccctga ccctcgccga gcccctgccc cgggatctcc
     8641 tgcaacgccg tctcgacgcc tcccagctgg tgttctcgcg ccagagtttc caggtcgtcg
     8701 cggtgctcga cgatctcccc gaacgggatc gcctgacgcc gctggtgtcc gccatcgcgg
     8761 ccggcggcga acgcttctgc cgcctcctgc tggaaacccc ggacaccaac gagggcaagg
     8821 aaacctcggg cttcctccgg cgcttcgaac cgctcctcga cgcggcgctc cgtgacgcgg
     8881 gcctgctgcg ccccgactcg ccccacctgc cgcgcctcca cgtcttcttt gcgaacgccc
     8941 gccacgtctg gctggggttc agcgcgccga gcgacggctc cgcctggccc atgggcattc
     9001 cgcgcctgcg catgccgaag gacgcgccga gccgctccac cctcaagctc gccgaagcct
     9061 tcctcaccct gctcacaccg gacgaacaga aagccaccct gcgaccgggc ctgcaggcag
     9121 tggacctggg cgccgcgccc ggcggctgga gcttccagct cgccagccat ggtctgcgcg
     9181 tcaccgccct ggacaacggc cccctcgcgc cggccgccct cgccaccggg ctcatcgagc
     9241 acctgcgcgc cgacggcttc acctggaagc cgcgcaaacc cgtcgaatgg atggtctgcg
     9301 acatggtcga gcaacccgcc cgcatcgcac cgctgatggc cgaatggctg gccagcggac
     9361 gctgccggcg cagcctcttc aacctcaagc tcccgatgaa gcggcgttac gaggagctgg
     9421 agcgctgccg cgccctcatc gaccagcgcc tgcgccacgc gggcatcaaa tacgtcttgc
     9481 gaatcaaaca cctttaccac gaccgggaag aggtcacctg ctatctgaca cggcgctgaa
     9541 gtgccgacca tgtataattc aggtttcctg atttggtgca ctgcaaaaac actgcacccc
     9601 aacgcgatcg atccatgtcg ttttccgaac tcgggctgat tcccgaaatt ctccaggcgg
     9661 tggccgatgc cggctacacc gaacctaccc cgatccagcg ccaggccatc ccggtcgtcc
     9721 ttgccggtca cgacgtcatg ggcggagccc agaccggcac cggcaagacc gcgggcttca
     9781 cgctgcccct cctgaaccgc ctcgcccgcc acgccagcgc ctcggcgagc ccggcacgcc
     9841 atccggtgcg cgcactgatc ctcgcaccca cgcgcgaact ggccatgcag gtgtacgaat
     9901 ccgtgcggat gtactctaag cacctgcccc tgcgcagcgt gtgcatctat ggcggcgtgg
     9961 acatgaaccc gcagatccag gagctgcgcc gcggcatcga gatcgtcgtc gccacgcccg
    10021 gtcgcctgct cgaccacgtg cagcagaaga ccatcgtcct caaccaggtg gaattcctgg
    10081 tgctcgacga ggccgaccgg atgctcgaca tgggcttcat cccggacatc cgccgcatcc
    10141 tcgcgctgct cccggcccag cgccagagcc tgctgttctc ggccaccttt tccgacgaga
    10201 tcaagaagct cgccgaccag atgctcaaga acccgcagct ggtcgaggtc gcccgccgca
    10261 acatggtcag cgagaccatc acccacgtgg tgcacccggt ttccagcggc ctcaagcgca
    10321 acctgctcgc ccatctgatc cgccacgaag aagaagccca ggtgctggtc ttcgtggaca
    10381 ccaagttcgg ctgcagccgc ctggcccact tcctggaacg tcacggcatc tccgccgacg
    10441 ccatccacgg cgacaagagc cagcaggccc gcaccgagac cctcgagaac ttcaagtccg
    10501 gcaagacccg cgtactggtc gccaccgacg tcgccgcccg cggcctcgac atcgaggacc
    10561 tgccctcggt catcaacttc gaactgcccc acaccgccga agactacgtc caccgcatcg
    10621 gccgtaccgg ccgtgccggc aagacgggca aggccgtgtc gctggtcagc gcggaggagc
    10681 gtcatcgtct cgccgacatc cagaagctca tcaaggtgga gatccgccag gaagtcgtcg
    10741 ccggttacga ccccgaaccc gatttcttcg aggccgacgg caagcgccgc cgccacgccg
    10801 cctccgcacc caaggccgag gccggcggcg aaccccgcga acgccacgaa cgtcgcgagc
    10861 gtcgcccgga acggagcccg cgggaatcct ctggccgcgg cggtccccgg acgccccaca
    10921 tcgcgccgga cggtttcgac ttcagcaagc cctatgagca gcgtgagacg ccgccccaca
    10981 cgcttgccga cgccgccgat gccagtccca agaaggctcg caagccgatc gccttcctgc
    11041 ttggcggcct cggccggcgc gactgacgca ggcatctccc acggcgtcca aaccagcgcc
    11101 attgcaaaga aaaggccacg cgatgcgtgg ccttttcaca tgccgcccaa tccgcagccg
    11161 cgtcacttgc ggcgcagaaa gaactcgaaa ctctggtccg gccgctcgtt ctgctcgatc
    11221 agttcgtgcc ccgtctgtcg tgcgaatgcc gcaaaatcct ttgccgagcc cggatccgtc
    11281 gcgagcacgc gcagcacctg ccccgcctgc aattcggcca gagccttctt ggcccgcagg
    11341 atcggcagcg ggcaattcaa accgcgggca tccacttcct tgtcgaacac catctcgaat
    11401 cctcttcacc gcagatgaca ggaggcgatt ctaacgtgca caccccacgc ccgcccgcca
    11461 gaacaccccc attcgagggg tgatttcggc acgacttcgc acacgtttag caaactttgc
    11521 gtttatatca aaatggcaaa ttctgacttt tgtcatctgt gaattacgtc accaattcaa
    11581 tcagaatagc gaagtcgggg atatctgact tcatggcata atcgtgctgc caaaatttca
    11641 tatgtcattt tcggatgacc ccgatgtctt gtccgaaaag ggcttttgcc catttcgggc
    11701 agcacccgaa gacattgccc cctcagtcac ttcaagaaag ccaaaaagat gcgctctaac
    11761 atgctccaga cggtcctcag cgacctgaac ggcacctccg ccgacatcga agcctccgcc
    11821 atcatctcct ccgacggcct catgatggcc gccctgctgc ccaacacggt cgatgaggac
    11881 cgcgtgggcg caatgagcgc cgccatgctc tccctgggcg accgcaccgc tcgagagctg
    11941 gcccgcggca acctcgaaca ggtgctgatc aagggcgaaa aaggctacgt cctgatgctc
    12001 caggccggcg ccgacgcagt gctcaccgtg atggccaagc cctccgccaa gctcgggctg
    12061 gtgttcctcg acgtcaagcg cgcagccgac tcgatcgccc gcatcctggg ttgaaagcga
    12121 ggagtcgagc atcatgactg acatggaccc cgcgtccatc gtcgggaacg cccgggagtg
    12181 cacgctgcag cacttccaga gcgtcgcccg caaggtgtac gtgaccgatg tggaaacgcc
    12241 ctacccccag ggcaaactga tcgtctcccg aaccaacgtg gagggcgtca tcacccactg
    12301 caaccagtcc ttcgtggaca tgtcgggcta cgaggaacac gaactcgtcg gacagcccca
    12361 ttgcatcctg cgtcatcccg acatgcccgc tgcagccttc cgtgatctgt gggaaacggt
    12421 gcaggcgggc cgccagtggc atggttatgt caagaacctg cgcaaggatg gcggctacta
    12481 ctgggtctat gccaccgttg tgcccaatgt ccgtgacggc cagatcgtcg gctacacctc
    12541 cgtccgccgc gaacccgccc gcaacaggat cgccgaagcc gaagccctct accggagcat
    12601 gagctgacca tgaccctcaa tttcacggtc agtccgagct ttcccaacga gcgcatctcg
    12661 gactggtaca tcttcaacac ctggctgcag cgtgccctgt ccgcgcaagt ccacctggaa
    12721 ctctacgacg gcttcggcgc ccagcgtcag gccatcgccg acggcaaggt ggacctcatc
    12781 tacgccaacc cgtttgatgc gtcgctgctg gtgcgcgaga agggattccg cgccgtcgca
    12841 ggtcccgatg accgcgccga cgagatggtg gtcttcacca ccgacgcatc gtccgcacgc
    12901 cagatcgccg acctgaagcc gggcacccgc ctggcctgca ccgacgaccc ggcggtcaat
    12961 accgtcggac gcatcctgct cgagccggcc ggtctgggtc ccgacaacac ggtcaccgtg
    13021 cagaaggaga cctatgtcca ggtcgcccgc gatgtcctgc agggccatgt ggatgtgggc
    13081 ttcatgcccg tcgaagcctt cgaaggcctc tcccgtctag tacggcgcga cctgcgtccg
    13141 ctggtcacga gccagttgca cgcgctcttt cacgtgttcc tggtcggtcc ggccctggcg
    13201 gcacgcagcg acgagatcgc cgagctcctg accggactca acgactccga caagggtcgc
    13261 cagatgctcg ccgcgctcgg cagcacgcac tggcggcggg tttcccagga agatgccgaa
    13321 ttcatgatcg acatcatctc gaccctcgcc cagtgagcct gttcatcacc gacacggcgc
    13381 agcccggacg gatccgtccc ccctccgtcc cggcggtctt cggactgccg gtacggaccc
    13441 tgaacatcgc ttcccattgg cacagcccgg ccaccggcgc actgctcgcc gccgagatcg
    13501 gccgcacacc ggccaatctg cacgcccacg tccagcgcat ctccctgtgg agcagcctgg
    13561 gcgactcgcg ggaactgatc gccgcgatca tcgatctgtg gatggtgctc ggcccccatg
    13621 gaatggacct gcgcaagcgg atgctcgaca cccatcggga gatcctcgag cgcgaagggc
    13681 ttgccgcgtg gatgagtgcc cagctcagca ccggcgccga ccgccacgat cccctgctgg
    13741 cccgcccggg cgttgtgctg gcgcgccccg tccgcggcat ccggcgattt ctgcgaactc
    13801 ccgccactct gtctgctgcc cggacatgag cagcaatgaa accccggcag gcatgcttca
    13861 gctcatccgg cacgccctct cgtttcccgg cccgtcctcg ccctcctcgc cgacgccaca
    13921 cgccatgaaa caccccccgc gcccttccgc ccgcagccag cccctggccc tgcatctcct
    13981 cgaagtaggc agcatggacc aacggctcat cggtctgtcg gtcgaacggc acatgaaaca
    14041 tgtccttcgc ctggtgaccg aacccgacca ggcccgggcc gtcctcatcg attgcgaccg
    14101 ccagggcgcg caggacgccc tgcgccaggc acggctcctg cgcggcgtgg ggatcgtggg
    14161 ttatgccttc aacccgcggg agcgcaacaa cgcctttccg ggcatcgaga tcctcaacaa
    14221 gccgctggcc ctggaccgcc tgcccgaagc actggatcgg gccatcgcgg cggccggtgc
    14281 ggctccgccg acaatcccgg cggattctcc cccgccggca gccccccccg ccccccccac
    14341 cctgggtgaa ggcgttcagg cggacgacgc ggacctgtgt ggcgcactcg aagatcttcc
    14401 acccgccccg ggcgccccgc tgccggacaa ggttttcttc gatccctccg actacctgat
    14461 cgggaccctt gcccaggcgg tcaggaccac cgccgagctc ggtcggcccc aggccatcgc
    14521 cggactgccg cggatcatcc gcatcgaacc gaagcccgcc cccacctgca tcgccggcta
    14581 ccgggacaat cagctccggc ccctgtccat ggcccaactc ccccaaagca cggcccgtat
    14641 cgtgccggcc ccgtcgttcc gggaacacat cgccggtgag gtcgcctgcg ccgcggaaga
    14701 gctcctctgg aatgtggccg cctggggcgc ccgcggacgg attcccgcag gccacgaccc
    14761 ctaccgcccg gtccgactcc gggcctggcc gaatttcccc gccatcttcg tgtcccccca
    14821 cgccctgcgt atcgctgccc tgtgggttcg cagccatgcg accccggtcg agatcgccag
    14881 ccggctcgcg attccgcagc gctatgtctt ttcgttctac agtgcggcac atttttcggg
    14941 cctcctggac cttcagccac caccgcccac gccggctccc gtagaatccg cgccgcagac
    15001 ggaacgcgtg ccggacacac cggtcgcgtc gcgacatcca gccccgtcgc cccagaaacc
    15061 gtcactgctt tcgcgaatcc tgaggaaact cctgaatgcg gtctgaacgc gcccgccaca
    15121 acaagatcgt cttctcgggc accgtcggtg caggcaagag cacggccatc gccgccctca
    15181 gcgacatccc gccggtgagc accgaagccg acgcaaccga cgagacggcc gacatcaaga
    15241 agaccaccac agtcgccatg gattacgggc tcctgagcct gcccgacggc gaaaaggtca
    15301 tgctctacgg cacccccggc caggagcgct tcagcttcat gtgggaaatc ctgtcggaag
    15361 gcggcatcgg cctgatcctc ctgatcaaca acgacacccc cgcccccctg gacgacctgg
    15421 agcgctacct caaggccttc aagaacttca tcgcgtccac tgccgttgcc attggcgtca
    15481 cccacatgga caaggccccc gtacccagcc ttgacaccta ccgggccaag ctcgccgaag
    15541 tcggcctcca ccccatgccg gccgtcttcc gcgtcgatgc ccgcagccgg gaagacgtgg
    15601 cgacgctcgt caaggccctc ctcttcaccc tgaacccggg tctgatcgat gcttgagata
    15661 atccccggct cctacctcgc cgtgacgccc tacggcgcct ggcgggctgt gtcccgtgcc
    15721 gacgactcca tcgaacggcg cattctgcag gccatcctgt gcgaggccca gtccccgatg
    15781 ctgagcgccg aggccgcctg cgcgtggacc ggcgagagca acgccgaagc cgcgctcggt
    15841 catctcctgc gcctgcagga ggacgcgagc ctggaagcct ggaccacgcc gcgtaaagtg
    15901 ccgtttggct ccatggaggc cagcctgccg cccctcctcg ccaccctcag cgatacccgt
    15961 cgggccgtgc tggccgaccg catgggcttc tgcctcgcca tggccggttt cccccaggag
    16021 caaggcgacg cccttgcggc cctcggcgcc gacatcctgt cggtggccga acgccagagc
    16081 gctgccctgg cccagatcgg cgaccagctt ctggacggct ggggcaacgt ggacgccgcc
    16141 ggagtcagtg gattgtcctt ctggcccatg cacgtgggac gcatccgtct cgccctcacc
    16201 gtgagcggcc gtccccgctt caatcaggcc acgttctcgg acctcgtttg gtccctgtgc
    16261 gtccgctacg gccgcatctg acaccactcg gtcccacgcc caccggatgc cgcgtacccg
    16321 cgatcacaca atcgccgcag gactctgact ccgtcgcacc cgccgggacg gccgacgcga
    16381 caccgtctgt gcgcgcgcac gccgaattca tccccaccgc agtagctcag acgggcccgc
    16441 acgtagccgg cgtcgcctac caaccgacgc cgcaccgcct acgcacgttc gcacgttgca
    16501 ccgccggcgg ggccgcagca taccgaatgc cggcatcgca attgtccaac gaaatcgcgg
    16561 aatgatcgga tcgtccaggc aaagtcatgc caatgaccct taaagggatc cgctcaaaac
    16621 gacggtaaac gagaaatatg aaccgcaagg cggcattcga gcctggcctg tcgaatcgga
    16681 atgcacggca tgcaatctca tcgacgcgga ttgcccccgc caccacgagg attgccgcgc
    16741 cccgtatcca tcgattaccg aatgcccagc gccgggctga ccgccatccg atgcaagacc
    16801 atgaaaatgt gcgcaattca cctgatgcct cgcacaaccg ccacggcgat caccgaccgg
    16861 ctccgggcat ttaaaactcg cacgtgccga atggcggacc accgcgtcac cttgcattgc
    16921 ggcctcgccg tgtggcttta gcgagcccct tgcccgcaga ggcaccgtcg tcgaaatccg
    16981 atcgcgccgt agccgctgaa cagatcactc aggcaagcac tccccgatac acgggctgcc
    17041 gcgccggaca gtggccccca tgccacctgc gcacatcgcg agccgatgac tgcgcgaatc
    17101 cagcgcagga aatcagcggg tcatgaccga tcgcccgcga agcacccgac ccgacggaaa
    17161 cccatgaacg gcaagggttt tcaattggcg gcacgaactt tccggagagc gcagcaatca
    17221 aagtcagctt catgcaagac tttaacgctg ccacaaagca tatattcccc acccgctcac
    17281 ccggcgccgc accgattcca ataggcgctg ttgcctgcaa caatcaggca tccatacggg
    17341 tctcgaatcg ccttaatgtt gtgtcaaagg ccttgcccac gccaatattc accattttac
    17401 gctccaacac ctattcagct attgcaccac tattgcttta gtgatacgtt tcgcccactc
    17461 tcaacacgac aggtgcctta tgagccagct ggaagacctg aaaagcgaac tgaacaagat
    17521 cgatggcgaa attgccgctt tgtcgcaacg tcgcatcgac ctgcagacgc aaatcgatgc
    17581 cgaacagaag aaatatgccg aagaagccct cagcaagatt gtgggcgaac tcaagtcgct
    17641 gaatctcgac ccggccgaca ttgcgaaggc cctcggcgtt ccggtcacgc cgaccgccaa
    17701 gaagacgcgt gcgccgcgcg gttcggctcc cgtcaagagc aagggcgaac cgaagtatcg
    17761 caacagcgtc gatccttcca aaacgtggag cggcaagggc cgcaagcccg aatgggtgat
    17821 cacctatctg gccaatggcg gcaagcttga agacctgctg atccacaagg cctgagcgca
    17881 ccccggcctt tcgccgggac tgccccagca gtccttcaca gcccgcattc cgctgcgcag
    17941 gtagccggtg cgggctgttt gcatgacggc aacgccccga cggttttgcg tcggcaccgc
    18001 cgcattccac ccttgcgtgt catccgcgga ttccgcgatt ccatcggttc aacgacccca
    18061 tctgccatga gcgacccctt cacccacttc gacgcccagg gccaggccca tatggtggac
    18121 gtcgccgaca aagccgaaac ccgccgtgtc gcccgcgccg aaggctgcat cgtcatgcgc
    18181 cccgacaccc ttgcacgcat cctcgctggc gacaatcgca agggcgacgt gatcggcatc
    18241 gcccgcattg cagccatcca gggcgccaag cgcaccgccg acctcattcc cctgtgccac
    18301 cccatccccc tcacgcgggt gagcgctgac ttcacggtcg aaggcgcgga caccatccgc
    18361 tgtaccgtca ccgcagaaac cgtggggcga accggtgtcg aaatggaggc gctcacggcc
    18421 gtgcagatcg gcctcctgac catctacgac atgtgcaagg ccgtggaccg gggcatgctc
    18481 atgcaaggca tccgcctcct cgaaaaggaa ggtggcaaat ccgggcactg ggtggccgaa
    18541 gcctgagcgc cgaccggctc ccgaccctgc accgcgaggc cggatttcgg ccggcacgcg
    18601 gacccgcgtg ggctggcgtc ggcgggcccc cgcatcgccg ccgggccgat gtccggatcg
    18661 acctggtcaa tcgggcgccg tgccgccatc acccgccccg atcgggcggc agttgcgtcc
    18721 tgccctgccc gcccataggg ctccgtgccg cacgccccaa aacagggcat gcgcaagaca
    18781 ggggtcaagc ttcgggtcta gaatgggcgc cgccggaacg ccctgccggt gcctcccctg
    18841 atagatggca tcatgagcca cgaaatcgaa ctgaagctga ccctccccaa aaaggcgctc
    18901 gcggccctgc gcaagcaccc cctcctggcc gaagcccccc gcgaaggccg cacctgcacc
    18961 ctcgaaaaca cctactacga cacgccggag ctggagctgc gcgagcggcg catggccgtg
    19021 cgcacccgca aggccggcag tcgctggctg cagaccgtca aatgcgcggc cgaatccgtc
    19081 ggtggcctga gtgcccgccc ggaatgggaa cagccctacg ttaacgccag cttcgacttt
    19141 tctaccgtgg accgggacga cgtgcgcgag cgcctcgaaa cctaccaccc gcgcctcgtc
    19201 cccgtcttca ccacccagtt ccggcgcgag accgtagtgc tcacgccacg ccccggcacc
    19261 cgcgtgctgg cgatgatcga cagcggcacc atcgaagccg gcgagcgcag cgcagcgctg
    19321 tgcgaactgg aactggaact cgtcgacggc gacccccgcg acctctacgc catcgccctg
    19381 caactcgccg agaccctgcc cctcacgccc gagaacgtca gcaaggccga gcgcggctac
    19441 cgcctgtact ccaacctcgc cctgaggcct gccaaagccc ggccgtcgcg cctcagcgcc
    19501 gagctctcgc cggtggacgc cttccgcctg atcgccttcg actgcctggc cctgtggcag
    19561 gccaacgaat ccggcgcccg cgaaagcgac gacccggaat tcatccacca gctccgcgtc
    19621 gccctgcgcc gcctgcgctc tgccctgcgc ctgttcggac cggccctgcc cgatgacttc
    19681 acacgacgct ggtccgacgg tctcaaggag gccgccgacg gcctgggtgc cgcccgcgac
    19741 ctggacgtga tgctcgccga agtgctcagc ccggtggccg aatccggcct cgccgacgaa
    19801 gccacccaac gcctcatcgc catggccgtg gatcgtcgcg accgggaaaa aaccgccgcc
    19861 cgcgcccagc tcctcgccgc cagccacggt cgccagatgc tcgccctcgc ggcagatctg
    19921 tatgccctgg agagcgattc cctggtcaaa tccgcccagc tctccacttt cgcccgcctg
    19981 cagctgagcc agctgcgcaa gcgcgcccgc aagcgctaca gcgccgccgg cgacctacag
    20041 gccgaaaagc tccatgaact gcgcgtggcc ctcaagcgcc tgcgctacgg catcgaattc
    20101 ctgctcccgc tgctgcccca aaaggccacc gtgcgctacg ccaacgccct cgccgacgcc
    20161 caggagaccc tcggctacct caacgacgaa gccgtcgccc gccgccgcct gcgggactgg
    20221 gccggcaagg atccggaact gcgcagcgcc gcggcattcg tggccggctg gcacgccccg
    20281 cgcatcgccc gcctgcgccg caacgtgctg cccggcctgg aagccctcct gtggggcaag
    20341 gctccgtgga gccgttgaag cgtgtcatcg tccggtaaca ccccaccccc agactgcccg
    20401 cctctttggg gagaagagga gacacctacc atggatctgc tgctgtggcg tcacgccgaa
    20461 gccgtggacg gcgttcccga cagcacgcgg gaactcagcg aacggggcct gcgccaggcc
    20521 cataagatgg ccgaatggct ggacaagcgc cggcccaagc gcctgcgcgt gctggtcagc
    20581 cccacgctgc gcacgcgcca gacggcgagc gccttcaccc acgatttcga agtcgtcccg
    20641 gcactcggcc cgggcaacga catcgcctcc atcctcgccg ccaccggctg gccggatgcc
    20701 ggcggtgccg tgctgatcgt cggccaccaa cccgaactgg gccgtctcgc cgcgctgctg
    20761 ctggccggcc aggaggccga ctggacgatc aagaaaggcg cgctgtggtg gttcaccaat
    20821 cgcgtccgcg acgaggagac ccagaccgtc ctgcgcgccg tggtgcctgc cgacctggcc
    20881 tgatccccgc agcatgaaaa aagccgcccg cgggcggctt ttttgttggc gcagcgccgg
    20941 ggtcagcggg ccggcaccat gatctcggct tcgccttcga ccacattctt gccgcgcacg
    21001 gagcacaccg tgtcgaactt cgccttgcgc ttttcgggca ccagttcctt gatggtgacg
    21061 cgggtcacca cggtgtcgcc gatcttcacc ggcgccttga acttgagcga ctgggacagg
    21121 tagatcgcgc ccgggccggg caacttcatg ccgaacaccg tggagatgaa ggccgccgac
    21181 agcatgccgt gggcgatgcg gccgccgaac atggtggatt cggccagttc ctggttgatg
    21241 tgcaccgggt tgtagtcgcc ggacacaccg gcaaacatca ggatatccgc ttcggtcacg
    21301 gtacgcgcgt agacggcgct ctgcccgaca gagagctcct cgaacgtgta gccgtagaat
    21361 tcttcaaact gcttgctctg atcgctcatg gctgttccct taacgtgggt tcttggttgt
    21421 gttcgattct cgatccgtcc cgggcacacc gcccggaacg gatgcttcgc cagcataccg
    21481 caatccgccg tacggcgaag ccccgtttcc atgacagttc cccggggcca cgccaagcgc
    21541 ttgggctaga atctcggtca aagcccgccc ccctaaagca ccatgtccca gcccgaccag
    21601 gccgttctcg accgactcct gcgcggaatc gacattcctc cctgccccgc cgtcctcacc
    21661 gccctgcagc gggaagtggc cgcccccaac gcctccctga cccgcatcgc gcagatcatc
    21721 aatggcgacg tatcccttgc cgcttccgtt ctgaaggcgg ccaactcgcc cgccttcggc
    21781 ctgtcgcgcc gggtgggcaa cgtggcccag gccgtgcccg tgctgggcat gcgcaccctg
    21841 tcggcaatca ccgcgggcgt cgcgctgcgc accgccctgt ccggcggcgc ccgccggctc
    21901 gagcgcttct gggactcatc cagcaagacg gccctgctct gcgcccgcct gacccgcgag
    21961 ttcaccggcg taccggccga gaccgcctac accttcgggc tcttccacga ttgcggcatc
    22021 cccgtgctga tccaccgctt cccggattac ctcgacacgc tccagcttgc gaacggcagc
    22081 ttcgacctca gcttcaccga agtcgaggaa cgccgccatg ccacccacca cgcggccatc
    22141 ggcaccctgc tcacccgcaa ctgggggctg cccgaagaca tcagcacggc gatcctgctt
    22201 caccacgacc tggacatctt ccgccccgat gccgaccaca tcgccgcccg cgcccgtacc
    22261 ctggtggccc tgggcgccct cgccagctat ttcgtgggca gttccctgcg catgagcgaa
    22321 gacccctggt ggattcgcgg acgccccctg tatctggcgc acctgggcct cggggacaag
    22381 gacttcctcg acctgcgcga gacggccttc gaggccatcg aatcgttctg accggatccc
    22441 cgccatgccc gcctggccgc tctatcccgt gtccgccctg cgcgccgtcg aagccctcgc
    22501 cctgcccggc gcccggccgc cgctgatgga acgtgccggg gccgccgcag ccgacctcgc
    22561 ccgacggctc ctcggaagct ccgcgggcga cgtgctggtg ctgtgcggac cgggcaacaa
    22621 tgggggcgac ggattcgtgc tcgcccgcca ccttctccag gccgggcacc gggtctgcgt
    22681 cgtctgcacg gcggacccgg cacgcctgcc ggccgacgca gcggcggccc ggcagacctg
    22741 gctcgacgcc ggcggcacca cgcagacgca cttccccgcc gcgcccgcct ccggctgggc
    22801 cctcgccgtg gacgccctct tcggcatcgg cctgcaacgt ccgccgaccg ggcaatacgc
    22861 cgcgtggatc gaggcattca acgcactgga ctgtccccgc ctggccctgg acatccccag
    22921 cgggctcgat gccgacagcg ggcgacgcct ggaggtctgc gtcgacgcca cccacaccgt
    22981 caccttcatc gccgccaaac ccggcgtgtg caccctggat ggccccgaca cctgcggcgt
    23041 actggagatc gcccccctcg gtctcgaccc cgaagccctg gtccccgcac cgggccgcac
    23101 gatctcgcca gcgcttttcg ccgagtccct gcggctgcgc cgtcgcaaca cccacaaggg
    23161 cagcttcggt gacgcggcac tgatcggcgg cgcaccgggc atggtcggtg ccgcactcct
    23221 cgccggccgt gccgcggcct ttctgggtgc cggacgggtg ctggtcgggc tcctcgcggc
    23281 cgacgccctg gcagtggacc cgctgcgccc cgaactcatg ttccgtaacg cccacggcct
    23341 gctcgccgaa aacgccggcg cctacgccat cggccccggc ctgggccaat ccgacgccgc
    23401 acggtcgatc acggccgatg ccctgcgcct gcagcgcccc ctggtgctgg acgcggacgc
    23461 cctcaatctc gtggcgcgac acgcggaact gcagcacgcc ctcaccgccc gcgacgcgcc
    23521 caccctcctc accccgcacc ccgccgaggc ggcgcgcctg ctggcctgct ccacggccga
    23581 catccagacc gaccgcatcg cccaggccct cgcccttgcc caccgattca acgcactggt
    23641 ggtgctcaaa ggttgcggca gcgtcgtcgc cgcgccggac ggtgcctggt ggatcaacac
    23701 cagcggcaac ccggccatgg ccacggccgg catgggcgac gtcctcacgg gcctgatcgt
    23761 cgccctcctc gcccagggct ggcctgcccg ctcggccctg ctggcgggcg tccatctcca
    23821 cggatgtgcg gccgatctgt gctgcgccga gcagggcctg cacgcggggc tgctggccgg
    23881 cgagatcctg gaacccgcgc gccgctgcct caaccgctgg ctgggtgcgc cggaagcacg
    23941 gccatgaacg gtaccgcctt cgaccccgcc ggacgcagca tccgctcgat ccgcctcttc
    24001 ccccttcccc cggtcgacgc gacgatgccg ctcaccgatg ccctgcgcgt cctgctcgat
    24061 cagaacgtac ctgccgccct ggtgcgcctg cagggtacgg tgatcggcat cctgtccgag
    24121 cgcagtgcgg tcggcgcgtg tctgggcgcc gacgcatccc gactcaccgc ccgggacgcc
    24181 tgcctgctca atccacctgt ctgcggtccg gacaccgagc tgcgcgaggc ctaccgcctc
    24241 ctcgtccgcc acgacagccg cgatctgctg gtgtgcgaag cggacggacg gcctaccggg
    24301 ctggtggccg agagcgacat tctctcggcg ctgggcatgg agcattacgc ccacctggac
    24361 caggtcggcc aggtcatgag cctgcgcccc cgctcggtgg ccgagaccgc cagcaccgaa
    24421 gacgctttgc gtgtcatgcg gcgccacgac atcggcagcg tggtcgtcgt gaccgaccag
    24481 tggccggtcg gcatcgtcac cttccacgac ctcagcccgc gggtggcccg cggcgagcgc
    24541 ctgcacgaac tccccgtcac cgacgtgatg gtgcgcgacc ccatcaccat cgccccccac
    24601 acctccgtgt gggaggcctc ccagatcatg caccgggccc gcatccgcca cctgccggtg
    24661 gtacaccacg accgcctggt gggcatgctc gccgaacacg acatcgtcac cagcctggaa
    24721 ggccgctatg tggcctttct gcgggagatc atcgtcgaac aggaacggga actcatcgcc
    24781 cagcaggtcc acatcgcccg ccgcgagagc gaggagagcc tgcgccgcat catcgacgcc
    24841 tcgcccgaca tggtcatcgt caaggatccg cagggctgct ggcagatcgc caaccgggcc
    24901 gcgctcgaac tcttcggact cgaccacgac gcctgtcatg gccgcgccga agaagcgttc
    24961 gccgaccgcg ttccgcagcc ccgccgggag gccctcctgc gctacgccgc cgccagccgc
    25021 cacgcctggc agtccccgga cggcctgcgc cacagtgacc gcgtgccggc cgccgagggc
    25081 gccgacgtcc acgtggaatt catcgaaaag cccatgttcg gcgccgacgg cgccccatcg
    25141 atgctggtca ccctggggcg cgacatcacc gaccgggtcc gcgccgagga tgccgtccgc
    25201 gccctcaacg cgtccctcga agagcgggtc gcccagcgca cccgcgagct tcgcgccacc
    25261 ctcgccgaac tggagacttt caactactcc atctcccacg acctgagcac gcccctgagg
    25321 gccatcgacg gcttctcggg catcgtgatg caggattacg ccgaccagct cgacccccag
    25381 gccctcgaat acctgcgccg cgtgcgcgac gcctcccggc gcatggcgcg catgatcgac
    25441 gatctgctcg atctctcccg ccactcccgc aaacccctcg aacgcagccc gctggacctc
    25501 tctgccatgg cgcgcagcgt cgccgacgaa ctcgtccacg gcgacccggg ccgccaggcg
    25561 cacattcatg tcgagccggg catcgaggct gaaggcgacc ccggcctgct gcgcaccgtg
    25621 ctgcagaacc tgctgggcaa tgcgtggaag ttcacccgcc cgcaggcctg cgccgacatc
    25681 cgcttcagca tgcgccgccg gcaaggcgag cggatcttca cggtgagcga caacggcgtc
    25741 gggttcgacg ccgcccacgc cgcgcatctc ttcgtaccct tccagcgcta ccacgccgag
    25801 gaggacttcg aggggagcgg gatcggcctg tccaccgtgc agcgcatcat cagccgccac
    25861 ggcggccgga tctgggccga gagcgcgccc ggacagggct gcagcttcca cttcaccctc
    25921 ggctgacgca tgcacgcgcc gccacgcccg gcccggggcg tcctcttcgc catggtgtcg
    25981 ctggtgctct tcgtgtccct ggacgtcacc tccaagtacc tctcccagcg ctatcccgtg
    26041 ccgctgctga tctgggcgcg ctataccgtc cattgcctgc tgatggtgct cgtcctcggc
    26101 cccagccagc gcggccgtct gctcgccacc gggcggcccg ccctgcacgt gctgcgtgcc
    26161 ggcctgctgc tcgccaccag cctgtgcggg ctgtcggcct tccagcgcct gcccctcgcc
    26221 gaagccaccg cgattgcctt ctgctcgccc ctcatcgtca ccttgctggc ccgaccgctg
    26281 ctgggcgagc gggtcggtcc ggcccgctgg gtcgcagtac tggtcggctt tgccggcgtg
    26341 atgctggtgg cgcgcccggg cagtggtctg gtgcccgacg gtgtactctt cgccttcgcc
    26401 accgccgtgg tgtatgccct ctatcagctc ctcacgcgca agatggcggc gaccgagcgg
    26461 cctctcaccc tgctcttcta ctccgccctg gtcggcagcg cggtcatgtc cgccaccctg
    26521 ccctggctgg accgggggcc gacgccgagc ctcccggagc tgccgttatt cctcgccatg
    26581 ggcaccctcg ccgggctcgg ccacttctgc ctgacccgcg ccttccgtga agcccccgcg
    26641 tccctgctgt cgccgctact gtacggccag ctgttctggg cgacgctctt cggatgggcc
    26701 gtgttcggcc agtttccgga tcgcctcgcc ctgaccggca tgctcgccat cggcgcgtcg
    26761 ggcgtactca tcgccgtgga cagccgacgc ggcatgcgct gacggacgcc ctcagcgcgc
    26821 tttcaaggcg tccaccgcgg gtggatatcg ccccgccgcc gacgcccgca accagcgcag
    26881 cgcctcctcg ggatcgcggg gctgcccggc gcccgtctcc agcatcaccg ccagcgcgta
    26941 ctgggcttcc gcctcgcccg cttcggccgc ctgccggaac agccgggccg cctccgccag
    27001 atcccgcggg accccgcgac cggcctcgaa cagccaggcg aggcgcgtcg tcccgcggac
    27061 atcccccgcc gcggcgatgg cgcggtaggc atccacggcg tcggccagtt tgccgtccgc
    27121 ctccagccga cgggcctcgg caaggcgccg gtcagcccca ttcacggcca cggcgcggcc
    27181 ctccagcgca gccagctggg cccgtgcctc gtcgtctccc agatcggcgg cctgctgcaa
    27241 ccagcgccgc gccgcggccg gatcgggcgc cccgaggcgg gccgtctgca gcagggtgcc
    27301 caggcgcagc gccgcgttgc gactgccggc ctgcgccgcc cgttcataac gccggcgcgc
    27361 ctcggtgccg tcccgcggct cccccagccc gtcctccgca aggatgccca ggttgaacca
    27421 cgcctcaccc tggccccggt ccgccagggc cgtccagatc tgccgggctt ccgcgtaatg
    27481 ggccatcttg aactccgcat agcccttgta gaggccgatg gtcggcttgc gcaccacctc
    27541 ggccagctcg tcctcgtcaa gcagcgcggc ctccgcggaa acggcgcggc ccaggcacag
    27601 cgcgccgacg atgcagcaca ggccggcacg aaatcggaag gcgtgcatgg tgagactcct
    27661 ctgtctgccg tggccgcaac cccatcgcga ccctcgccgg cttggagtac ggcgtggggt
    27721 cccggagttc ccccggtgcc cgccatccgt accggccgtg ccgtgcacgc agcggtggcc
    27781 cggtgcgcgc gccatgcccc cggtctgcgc gagcccgctc cccctccccg cccggtgtta
    27841 gaatcgcggc ctgttttctc tcaggcgact ccgatggaat ttgcagatca gcttcgcgtt
    27901 gaagtccagc gctccgtggc ggcctccctg gccgaagaca tcggcaccgg cgacctgacc
    27961 gcccgcctca tccccgccgc ccgcgaagcc cgtggccgcg tgatcacccg cgaagacgcc
    28021 gtgctgtgcg gcaccgcctg gttcgacgcc gccttcgccg cgctggagcc cagcgcctcg
    28081 gtcatctggc atgcccgcga cggcgagcgg gtcaagcccg gccagctgct ctgcgaagtc
    28141 gacgccagcg cccgcgccct gctcaccgcc gagcgcaccg cgctgaactt cgtccagctc
    28201 ctgtccggca ccgccaccct caccgccacc ttcgtcgaag ccgtggccgg cacccgcgcc
    28261 aggatcgtcg acacccgcaa gaccctgccc ggcctgcgcc tggcgcagaa gtacgcggtc
    28321 aaggtgggcg gcggcaccaa ccaccgcatc ggcctctacg acggcatcct catcaaggaa
    28381 aaccacatca tcgccgccgg cagcatccgc gcggtgatcg agcaggcccg cgccatcgcc
    28441 ccgtccaacg tcttcatcga ggtcgaggtg gaaaccctcg accagctccg cgaagccctc
    28501 gccgccggcg cccgcatgat cctgctggac aacatggacc tggaccagat gcgcgaagcc
    28561 gtgcgcctca acgaaggtcg cgccgagctg gaagcctccg gcggcgtcaa tctgcaacgc
    28621 gtccgcgcca tcgccgaaac cggggtcgac cgcatctcca tcggcagcct caccaaggac
    28681 gtgcgcgcca tcgacctgtc cctgcgtcac atcgaagagt aagccccacc cgccaggccc
    28741 ggccgagcac cgggtccgcg ccagccctgc gcctgccatg agaatcgaaa acgacatcaa
    28801 tgtcgccccg tccgaaagcc ccaccgtcgc cgacgccgtc gaaaagctcg tgctgcgcat
    28861 cacggcccgc gacaccggca tcgacctgtc ggccgtcacc cgcatcctga tcaccgccga
    28921 cctgcccgac gccacccaac gcttcggcgg cacgccggta acccccgatc agctgacccg
    28981 catcgtccgc gagggccagg ggtacatgct gcttttcagt ggcgtcagga tgctggagat
    29041 gttcaagggc ggcgtcgctg aagtctcccg cgtgatccat cacctgcaca aggacctgtg
    29101 gcacctccac gacgtggccc ggccgcggac gcccaagccc accaccccgc tggcccgcgc
    29161 cctcgcgccg atctgcgcca tcatgtggca ggaataccgg gtcaaccggc gcaccgcctg
    29221 gtccgtgccg cgggattccg atctgttgat gcaccacctg gccgccctcc tccaggcgct
    29281 gcccggcggc atgcaggaag agctcaccct gtacttcgcc gaccaggacc tggacgccct
    29341 cttcgccaag agcctggggc gcatttccca cctcatgcag gcggtcgccc acgtgcaggg
    29401 ctacctggac ggcattggcc agccgctgca gaccatcagc cccgacatgc acgaggtggt
    29461 gaaccagagc ttcctcgcgc ccctgtggac gaccaccgcc cgacacctgg ccgcgggcta
    29521 cgagcactat ccggcagaag tggccgacct gcccctgcgc cgcgacatcc agaacgcctt
    29581 cgccaccttc ggcctcacca tccgcgaaaa cgacgcgggc gacgacgtgt ggctgcaagt
    29641 cgaaccgccc gtcccgcccg aaaagatgca ctgagacccc ggcgccgggc tgctgccgcg
    29701 cgtcagaagg tgatgacctt gtccgcgtcc agggtcgcct gggccagctc cggcagggtg
    29761 ccgatggtgg tgccggggat caggggcaga tccccgagcc cccgcgccag ggcgcaggtg
    29821 cggcacaggc ggatcgccac cccgttgcgc accagctcct ccaccatgcc ctgcagcccg
    29881 tttccgttcg cgtcctgctg atggggcaag gccatcaccg tggcgtccga catcaggaag
    29941 agctccacct tcggctgcgc gtcgctgccc gccagggcga tggccagacg cagcgcggac
    30001 aggcagcgct cgctgccgta gggaggcgca tggacgatga tcagaatctt ctgcatggtg
    30061 atgtccttca aagaaaaggg aatgccgcac tgctcgccgg cccggtggcg cacggccgac
    30121 cggcgggcag catcagtaat ggggcgggat ctcgtcccgc agggaatggg gctcggcggt
    30181 actggccgac gtgctgtgca cctgcttggc gagcgccagc agctgccgct gcaaggcgtc
    30241 aatctgctcc tgctggcgat gcacggtcag attgagggtc tccaccagat cttcggtgag
    30301 agccagcttg atttcgatct cggtcagtcg cgattccatg ggcgtttccg ttccggcaga
    30361 gggccgaccc ggattgttcc agccagcggc gccggcgcca agcccttgcg acaccgatcc
    30421 gatgcacctt gacccgccaa gggtcgtcac ctaagctggg gactgtccca agggggagta
    30481 gctcttccgc cgtggcgtcg tcagtacggg caggtttctc ctgccccggc gtcacgggtt
    30541 gccggcccag tccggcagcg agcaagacct ttgccgcgat ggcgaaggtc tcatggcact
    30601 ccctccccgg atccaacggc ccttcgtcct cgctggacaa agggccgttc tttcgtggca
    30661 tgccgccacg gaacggcgga tgccatccgg acctggagac agcactcatg gaatcaatcg
    30721 gaacctggtg gatgtgggcc ggcttcttcg ccctcgtcct ggtcatgctc gccatcgacc
    30781 tctttgccgt gggtggcgga cgccgccatc gggtgtccct gcgggaagcc ggcctctggt
    30841 cggccatctg ggtcggcgtc accctcgcct ttgccggcgc cctgtgggtc tggctggacg
    30901 gcagcgccgg gcgcgagacg gccaccacgc gcacgctgga attcctcacc ggctatctca
    30961 tcgaaaaatc cctggccgtg gacaacgtct tcgtgtggct gatgctcttc agcttcttcg
    31021 gcattcccct agaactgcag aagcgggtgc tcacctacgg cgtgctcggc gccctcgtgc
    31081 tgcgcacgct catgatcctg ggcggcgcct ggctgatcgt ccagttccac tggctgctct
    31141 acgtcttcgg agccttcctg ctgctcaccg gcgtgaagat gctgtggctc gccgacgccc
    31201 gcccggatct ggccgccaac ccggtgctgc gctggatccg ccgtcacgtc ccggtcaccg
    31261 aggcgcttca cggcgagcgc ttcatcgtca ccctgcccgg caccaacggt ccggtccgtg
    31321 ccctcacgcc gctggccctg gccctggtga tggtcgaact gtcggacgtg atcttcgccg
    31381 tcgactccat ccccgccatc ttcgccgtca ccaccgatcc cttcatcgtg ctcacctcca
    31441 acctgttcgc gatcctgggc ctgcgggcaa tgtacttcct catcgccggc atggcggacc
    31501 gcttcgccct gctcaaatac ggcctcgccc tggtgctcgt gttcatcggc gccaagatgc
    31561 tcctgctgga cgtggtgaag atccccatcg ccctgtcgct caccgtggtg gcgctgctga
    31621 tcggcggtgc ggtgatgctg tccctgcacc gcgaacggcg cagggcgctg ccggagcctc
    31681 gacgcgacga tgggggcggg cgataatgcg cggttatcag ccagccgccg tccgagcggt
    31741 ccgcatccgc cccaggagag ccgccatgat ccccacccgc cagcacctcg aactgctcgc
    31801 accggccaaa aacgccgact tcggcatcga ggccatcaac catggcgccg acgccgtgta
    31861 catcggcggc cccgccttcg gcgcgcgggc ccaggccggc aacgaggtgg ccgacatcga
    31921 acgcctcgcc cgccacgccc accgctacgg cgcgcgggtg ctggtggccc tcaacaccat
    31981 cctccacgac gccgaactcg acggcgccga ggccctcatc cggcaggttt acgaggccgg
    32041 cgccgacgcg ctgatcgtgc aggacatggg cctgctggaa ctcgacctgc cgcccatcca
    32101 gctccacgcc agcacccaga ccgacatccg ccatccggaa aaagcccgct tcctgcagga
    32161 cgtgggcttc tcccagatcg tgctggcgcg ggagatgagc ctcaccgaga tccgcaaggt
    32221 cgccgccgtc accgactgcg ccctcgaatt cttcgtccac ggcgccctct gcgtcgcctt
    32281 ctcgggccag tgctacatca gccacgccca caccggccgc agcgccaacc ggggcgaatg
    32341 ctcccaggcc tgccgcctgc cctacgacct ggaagaccag gacggccacc tcgtcaccgg
    32401 caaccgccac ctcctgtcca tgaaggacaa cgaccagagc gccaacctgc gcgccctggc
    32461 cgacgccggc atcagcagct tcaagatcga aggccgctac aaggacatcg gctacgtcaa
    32521 gaacgtcacc gcccactatc gcaccctcct cgaccagctc ctcgaagacg cgcccgacta
    32581 ccgccgcagt tcctccggcc gctgcacctt cctgttcacc ccccagcccg aaaagacctt
    32641 caaccgcggc gccaccgact acttcgtgaa cggccgccag ggcgacatcg gcgccttcga
    32701 ttcccccaaa cacgtgggcg agcccgtcgg cagcatcctg cgcatcgacc cgcaacgccg
    32761 cagcttcgag ctcagcagcg ccgtcgagct gcacaacggc gacggcctca gcttctacga
    32821 ccgcaagggc gaactcatcg gcctgcgcat caaccgcgcc gaaaagctcg gtgacgactt
    32881 ccgcgtgcac accgccgacg ccgtgcccgc cgacctccgc cctggcagcg gcgtgttccg
    32941 caaccatgac cacgccttcg agcggctcat ggacaagaaa accgccgaac gccgcatcgc
    33001 cgtggacctg cacctcaccg acgacaccga cggcctcgtc ctgcaggtca ccgacgaaga
    33061 cggcttcagc gcccaggcgc gcctgcccca tcccctcgaa gccgcccaga acgccgagcg
    33121 cgcccgcagc gccctccacg acggcctcgc caagctcggc gccacgatct tcagcgcccg
    33181 cagcatccgc ctcgacctcg ccacgccctg gttcgtcccc gctgccgccc tcaacggcct
    33241 gcgccgcgac gccatcgaac gcctcgaagc cgcccgcctc gccggcctgc cacgcccgcc
    33301 gcggcgcgcc gccgtcgaac cgccggcgcc ctatccccag accgaactca gctacctggc
    33361 caacgtcctc aacgaacgcg cccgcagctt ctaccggcgc cacggcgtca ccctcatcga
    33421 agacgcctac gaagccaacc aggtgctcga cgacgcctcc ctgatgatca ccaagcactg
    33481 cctgcgctac agcttcaacc tgtgccccaa ggaagtgaaa ggcatccgcc ccgaccccat
    33541 gaccctcgtc aatggcaagg aacgcctcac cctgcgcttc gactgcaagc gctgcgagat
    33601 gcacgtcatc ggccgcctgc gtcccgccgt gctggacgcc gtcccgcagc ccgtgcgctt
    33661 ccacgcccag gcgccggccg ccctgcgcgc cggccgctga gccgcccacc accgcgatcc
    33721 ctccatgaag ctcgccatca ccctcatcgt cctcgccctg ctgatcatcg tcgggcccgc
    33781 cgcctggcaa ttcacccaca ccaaccccgc cgccgtgccc acctccggcc tgccctggca
    33841 gatcgacacc ccggcgcccg gcgaatcccg cgtgttcaac ctcaccctcg ggcgcagcac
    33901 cctcgccgac gcccgcacgc gctttggccc cgacgcccag ctcgccatcg tcgccacccc
    33961 cagcgaagcc ggcaacgtgg agctctacta cgaatccatc accgccggct tcatcgccgg
    34021 ccgcctcatc gtcaccgccg acctgcccgc cgccaccgtc gaaggcctgc gcgaacgggc
    34081 cgccaagacc gaatacatgc agagcaccac gcgcaaggcc accctcgccg aggccgacct
    34141 ccctgtcgcc ctcgccgcgc cgatccgcgc catcgccttc atccccagcg ccaacctgga
    34201 cgaagccatc gtcgtcgaac gcttcggcgc ccccgccgaa cgcctgcgca gcaacgacca
    34261 catcgaacac ctgctctacc cggccaaggg cctcgacgtg gtgctcgaca gtaagggcaa
    34321 ggaactcctc cagtacgtcg ccccggcccg tttcgacgcc ctgcgcacgc ccctgcgggc
    34381 cgccgccgac caggccgcga ccgccccgcc cgccgcggcc cccgacaaac cctgacggca
    34441 caccgattgc ttgcccggca ggattccccg tccggagcat tgcccatgag caccccgtcc
    34501 accgtccgcc acttcgtcac ctacagcggc gtcagcctgc ccctcaagct caccacgccc
    34561 ctcgaaggcg ccgatctcgc ccaccgcatc accttctacc gtgcccacta cgacgcggcc
    34621 gagcgtctgg ccctcgtcga gaaagtcgtg tacggtgaga tcgaatccac ccaccactac
    34681 acctactacc ccagcggcgt gttgcaggaa gcccgggttt gtcagatagc cgacgaagaa
    34741 acgacactca tggcctacga cgaaaccggc gccctcgtct ccaccacccg cctggaagac
    34801 tgaatcccgc atacgtggcc gtccagtccg tccaacgccg ccgctgtctc acctgcaacc
    34861 gctggggcgg acagcgccgg ccgggcgacg aacccgacac cgtcgaattt gacgaagccc
    34921 acgaccgcgg tccctgccag gacggcccct ggcacggcac cctgcgcggc ccgcgcaatg
    34981 cctgcggcca gtggctgcgc tggaccctga tcgtgcctgc tcccgacccc gccgaagcgc
    35041 ctgcccaagc gtccccgccc catgaacagc cctgacgatt ccgatctcct cgtcgcctgc
    35101 ctgtgcgccg cctggtgcgg cacctgccgc gactacgccg ccggcttcca cgccctcgcc
    35161 gaacgcttcc ccggcgcccg cttcctgtgg ctcgacgtgg aagacgaggc cgatctcctc
    35221 gacgactacg acgtggagaa cttccccacc ctcctcatcc agcgcggcga cgcggtgctt
    35281 ttcttcggca ccatgctgcc ccatcatgag ctcctcgaac ggaccgtgcg cgcctttgcc
    35341 agcatgacgc ccgacgaaag ccgccactac gcccaggccg acgccacccg gcgcacctgg
    35401 cagcacgacc gcaacctgcg tcgcgccctc gccgaacggg acgacgacgc ctaaaacaag
    35461 cacaacggga gacaccccat ggacacctgc gttttctgcc gcatcgtgcg cggcgaactg
    35521 cccgcctccg tcattcacga agacgaacac accctcgcct tcatgaacat catggccggc
    35581 aaccccggtc acgccctggt catcgtcaaa ccccacgtcg aaaacatcta caccctcgac
    35641 gacgccctcg ccgccgccgt cttccgcacc accacccgcg tcgccaaggc cgtgcgcgct
    35701 gccgccggct gcgaaggcgt cagcctcttc caggccaacg aagtggccgg cgggcagacc
    35761 gtcttccact tccacatcca cgtcctgccg cgcctccccg gcgacgccct gcccggcgta
    35821 tggcccgccg tcgcccccag ccgcgaagcg ctggacgcca tggccgcccg cctgcgcgcc
    35881 gccctgtaag gcacacggcg ccgcccgcca caccggcatt caattccgcc ggcccgccgg
    35941 ccgataaact tcccgtccac cgcgccgccg cctgccgtgc attccgcccg gcccgcggcg
    36001 gatgcctcca tccggtcgcc gccatgcaca tccccctgct cagcgccgaa gcccccatca
    36061 ccctgatcct catggtgatc gccggcctca tcatgctccg gcccctcgcc catctgcgcc
    36121 gctccctgat gaacaccggc gtgttctacg gcgcctgctt tgctggcgac ctcctcgccg
    36181 gcctcctcga aggcagcagc cagcccgccg ccgcccgctg gctgcgcgaa ctctgcgtct
    36241 tcggcgaagg gctcgccgtc gtccgcttcg tcggcctcac cctcttccag gtcatcctgc
    36301 cgcgcctgca cgtcaacatc tccggcatcc tcgccgacat cctcgtcatc gtcggctaca
    36361 tcctgtgggg cttcatccgc ctcagcatga gcggcgtcga attcaccgcc ctgttcacca
    36421 cctccgccgt cctcaccgcc gtcgtcgcca tctccatgca ggagaccctc ggcaacctcc
    36481 tcggcggcct cgccctgcag atggacaact ccctcgagat cggcgactgg atccagatgg
    36541 gcgacctcgc cggccgcgtc accgacatcc agtggcgcta caccgccatc gtcacccgcg
    36601 ccggcgaacg catggtggtg cccaacagcc acctcatgaa gaaccgctac gccatcgtct
    36661 gcgacaagcg ccagtccaat ccccaccagc gccgccacat ccccttcaac gtggatctct
    36721 ccgtgccccc cgcgcgggtc atcggcgccg tgctgcagga catcctcacc gcccgcatcc
    36781 ccaacgtcag cgccaccccc gcccccgcct gcctgctcat ggacttcggc cccggctttg
    36841 cgcgctacca gctgcgctac tggctcgacg accccggccc cgacgaaatc accgactccg
    36901 acgtgcgcag ccacatcctc gccgccctcc agcgcgagga catccgcctc gccgtcgccg
    36961 accagaccgt ccacctcgtc caggaaggca aagcccaccg cgccgaagtc caggcccgcg
    37021 aactcaaccg ccgcctccag gccatcaccc acatcgacct cttctgcaaa ctctccgaag
    37081 ccgaacagct cgaactcgcc cgccgcctcg tcaacgcccc cttcgcccgc ggcgacatcg
    37141 tcacccgcca gggcgccgtc gcccactggc tctacatcat cgtcagcggc gaagccgaag
    37201 cctggtggca accgcccgac ggcagccccc agcgcctcct cgaaaaacgc ggccccggca
    37261 ccgtcttcgg cgaactcggc ctgatgaccg gcgccccccg ccgcgccacc gtcatcgcca
    37321 ccaccgacgt ggaagcctac cgcctcgaca agaccggctt cgaacacatc atccgcgccc
    37381 gccccgagat cgccgaaggc atctccgcca tcctcgaacg ccgcctcagc cacttccagg
    37441 ccctcgaacg gggcgacggc gccccgcgcc ccgacggcaa caaacccgcc aacggcgaca
    37501 ccggcgtcat cggccgcaag atccgcgaat tcttcgggct gtagacgcac caaggccccg
    37561 ccgcccttgc ggagacgggg ccc
//
LOCUS       DAOQMM010000023        37538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_274_length_37538_cov_51.172131, whole genome shotgun sequence.
ACCESSION   DAOQMM010000023 DAOQMM010000000
VERSION     DAOQMM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 37538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37538
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_274_length_37538_cov_51.172131"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..215)
                     /locus_tag="PLH85_05980"
     tRNA            complement(128..215)
                     /locus_tag="PLH85_05980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(179..181),aa:Ser,seq:tga)
     gene            388..1074
                     /locus_tag="PLH85_05985"
     CDS             388..1074
                     /locus_tag="PLH85_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNB64143.1"
                     /translation="MQIDTYGQQTQMLGASTNRVLRNTYLLLALSMVPTIAGAMLGVQ
                     MQFSFFAGSPLMGFLLFMGISFAFFWGIERNKNSGLGVALLLGFTFFMGLMLSSLLRV
                     ALGFSNGGTLIALAGGGTAAIFGVMATVATVSKRDFSGLGKFLFVGVIVLLLASVANI
                     FFQVPALYLTISAIAVALFSAYILYDINDIVRGGETNYVTATLRVYLDVYNVFVNLLN
                     LLMAFGGDRD"
     gene            complement(1136..2449)
                     /gene="rlmD"
                     /locus_tag="PLH85_05990"
     CDS             complement(1136..2449)
                     /gene="rlmD"
                     /locus_tag="PLH85_05990"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNB64144.1"
                     /translation="MHVDAPREALIESLDHDGRGVARVDGKTVFIEGALPGERVEFVS
                     YRVKPKFELADVTRIVAPSAQRVAPPCVLFGECGGCSMQHLEPSAQLAAKQRVLENAL
                     WHLARVKADVLYPVISGAPWGYRFRARLSVRQVPSKGGVRIGFHQRRSSHIVDMRSCP
                     VLPTHVSVLLPALRDLVTALSVADRLPQIEVAVGEGLTVLVFRHLEPLTAADKDHLRA
                     FAESHGVQVWLQPFGPDSASRLHPVKAPGLAYTLPEFGVSMAFRPTDFTQVNMGINRL
                     LMRRAMLLLDPRPGERIADLFCGLGNFSLPIARLGATVVGVEGSDALVRRAAENAAAN
                     GLAERSEFHAANLFEATEDSLAALGRLDKLLIDPPREGAIAVVKALGADSPRRVVYVS
                     CNPATLARDAAVLVHEKGYRLKGAGIANMFPQTSHVESIALFERD"
     gene            2528..3658
                     /locus_tag="PLH85_05995"
     CDS             2528..3658
                     /locus_tag="PLH85_05995"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB64145.1"
                     /translation="MIDTLLGGLSVEQFLAEYWQKKPLLIRQAIPGFTGVVSRDDLFA
                     LARDPDVESRFIAHDGARWEVLHGPQKATDLRRKKQPWTVLVQGLNLFVPEGDALLHR
                     FDFIPQARLDDLMVSYAVDGGGVGPHFDNYDVFLLQGIGQRRWLISNQDDRSLIEDVP
                     LKILQDFRPVHDWVLESGDMLYLPPHWAHNGIALGECTTYSIGFRSPTYQELAQEFLV
                     YLQDAVQLEGLYADPDLKPHVHSAEIGDAMVDRVAEQLARITWTREDVRRFLGAYLTE
                     PKPHVFFDSPDEPLDHDSFLAQCNRAGYVLDARSLLLFSDGRFFLNGEAVSISHTDLP
                     VMIELADRRRISTVADLTQDGQALLYDWYCCGFAHPDSIESK"
     gene            complement(4317..5483)
                     /gene="bamC"
                     /locus_tag="PLH85_06000"
     CDS             complement(4317..5483)
                     /gene="bamC"
                     /locus_tag="PLH85_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNB64146.1"
                     /translation="MHSRTLASGSAVAMAIALAGCSGSLLESKKIDYKSASAAPVKSL
                     EIPPDLTAPTRDDRYTVPDVSPKGTATFSAYSAERTGQGAQVGATPGAAPTAASASGS
                     DKMHIERAGSQRWLVVQGSADKLWPQIRDFWQESGFLLNVDKPEIGVMETDWAENRAK
                     IEQGFIRNTLGKVLDGLYSTPERDKFRTRLEAGAEAGTVEIYISHRGMMEIYPTEGKD
                     RTIWQPRAADPELEAEFLQRLMVRLGADESRAKAQLATAPSSDKARLANAADGATLLT
                     VQDAFDRAWRRVGLALDRVGFTVEDRDRSQGLYYVRYVDPEVDVKKKGDEGLLSKLAF
                     WRSDKPALPTGSQYRIFVKNAGDESSVQVLTREGGVDKSDSARKILGLLYEQLK"
     gene            complement(5513..6391)
                     /gene="dapA"
                     /locus_tag="PLH85_06005"
     CDS             complement(5513..6391)
                     /gene="dapA"
                     /locus_tag="PLH85_06005"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNB64147.1"
                     /translation="MITGSIVAIVTPMNDDGSLDLPRLRTLIDFHVQEGTDGIVVVGT
                     TGESPTVDVDEHCELIRVCVEHAAGRIPVIAGTGANSTREAIELTEFSRKAGAVAGLS
                     VVPYYNRPTQEGLYRHFRAIAEASELPLILYNVPGRTVADLANDTALRLAEIPNIIGI
                     KDATGSLDRACDLIARAPRDFALYSGDDMTMMAFILLGGHGTISVTANVAPGLMHEVC
                     AAALQGDAVTARAANAKLVGLHRDLFCEANPIPVKWAVEQMGLIGPGIRLPLTRLSES
                     CHERVRKAMRQAGIEV"
     gene            complement(6564..7490)
                     /locus_tag="PLH85_06010"
     CDS             complement(6564..7490)
                     /locus_tag="PLH85_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNB64148.1"
                     /translation="MTSTAFITHRECWLHNMGAHHPESPERLAAINDRLIAAGLDMYL
                     SFYDAPVATAEQLSRAHPREHVDAILASAPEHGIRHLDPDTAVCPHSIKAALRSAGAG
                     VMATDLVMSGEVENAFCAVRPPGHHAEAAKAMGFCFFNNVAVAARHALDVHGLKRVAV
                     VDFDVHHGNGTEDIFKDDPRVLMVSIFQHPFYPYSGHDNPAPNMVNVPVKAGSRGDVF
                     REIVTNIWVPALRNHAPEAIFISAGFDAHYEDDMGSLGLVEKDYIFATEELKKVAAEF
                     SKRRIVSMLEGGYSLSSLARSVVAHVKSLADL"
     gene            7659..8576
                     /locus_tag="PLH85_06015"
     CDS             7659..8576
                     /locus_tag="PLH85_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB64149.1"
                     /translation="MPLYIANRLVDAAGRILLGKEQAIRLALACLLARGHLLIEDVPG
                     VGKTTLAHLLARLLGLQFQRIQFTSDLLPADILGVSVFDPRDGAFRFHPGPVFAQMIL
                     ADEINRATPKAQSALLEAMEEGQVSAEGRTLPLPAPFFVIATQNPADQIGTFPLPESQ
                     LDRFLMCIALGYPDPAAERALLAGEDRRALLARQETLLAPGELDTLFDAAARVHVAPP
                     LLDYVQALLAHSRQATGFSLGLSPRAGLGLVASARAWALLAGRDHVQPEDVQAVLPAV
                     AGHRLRSDGDRRTSPEERVRRLLEAVPLP"
     gene            8576..9562
                     /locus_tag="PLH85_06020"
     CDS             8576..9562
                     /locus_tag="PLH85_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB64150.1"
                     /translation="MPVHARLDALRARFQRWLFRVHGPEPAPIRLGQRRIFVMPTAFG
                     VALGVTLLVMLLASINYALSLGYALTFLLGGVGWVHIHLSFRNLVGLSIAPGRVEPVF
                     AGATASFGVLMTNPLPRSRLALRLWPARTGASPDEAFELPPDSQVRISVHVAAPQRGW
                     LPLPRLQLESRHPLGLIRAWSLFQPDLRALVFPTPEADPPPLPRGTGLRPGAHGRRQG
                     RDDFAGLRAHQPADPPRHVAWKALARSGEWLTKQFDGGGAEALWLDWNTLPPGLDTEQ
                     RLARLTRWLCDAEAAGLEYGLRLPQQTFPPASGPAHRLACLTALALFGGPDG"
     gene            9555..11495
                     /locus_tag="PLH85_06025"
     CDS             9555..11495
                     /locus_tag="PLH85_06025"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and DUF4129 domain-containing
                     transglutaminase family protein"
                     /protein_id="HNB64151.1"
                     /translation="MAERGLRPDQTGWLVVAAGVSLLPHAAQLPHWLSAVCALFLSWR
                     GMLAAQRARLPHRALLGTLGLAVAGGVLVEYGHLFGKDPGIALLAAFLGLKLLETDDI
                     RGGRAVILLTFFLQMGQFLHQQGMATAALALVGTVVSLATLAALAGRARPLRPRLTEA
                     ARLTVLSLPFMAVLFILFPRVQGPLWGLPADAFSALTGLSDTMTPGALARLSESGAIA
                     FRATFDGPPPPRAQRYWRGPVLTRFDGHTWRAAHLPARDVPAYAVSGRAYTYTLTLEP
                     HDRPWLLALDFPDAQAGLRYGADFQLLAREPVRSRLRQTLASYPDTPVGLDEPPILRR
                     LALALPAGANPRTVAAGSQLRANAVRADDIPARGIALMRDARLTYTLEPPPLGEDDVD
                     AFLFDTRQGFCEHFAAAFVVLMRAAGVPARVVTGYQGGEINPVDGSLEVRQSDAHAWA
                     EVWLAGRGWLRVDPTAASAPARIERGLAAALPDTGHLPLLMRPELAWLQGLQHHLDAL
                     NHRWNQWVLGYNPARQRDLLAGLGLGEVDWPQLTGLMTAACAAVTAALAGVLLRNRRR
                     DPVLRAWEGVCSRLAAHGLPRADWEGPTAYAARVGTTRPDLADAIAGLATDFARLRYG
                     EASDTAARRDRFLHSARRFRPR"
     gene            11492..12505
                     /gene="mltB"
                     /locus_tag="PLH85_06030"
     CDS             11492..12505
                     /gene="mltB"
                     /locus_tag="PLH85_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNB64152.1"
                     /translation="MILRPFRLRPALLLASALAAGPLAAAERYADRPDVQQFIAEMKE
                     RHGFDPDALTFIFRRATFQPAVIKAISPPKDPGVRSWQRYRGRFIEPVRIKAGVRFWD
                     AYAETIKAASDKYGVPEEIIVGIIGVETIYGRNTGSFQAVSALSTLAFDYPRRAELFR
                     GELESLLLMAREQGRDPLDYQGSYAGALGLPQFLPSSVRNYAVDFDGDGHIDLLASPK
                     DAIGSVARYLQMHGWERGGPVAIRAVTNADATLETLIANDITPAFDAEALAAHGVRAA
                     SGTPAVGPAAFVELVTPGADSEYWLGYQNFYVITRYNRSSFYAMSVFQLAEAVKEARQ
                     ARR"
     gene            complement(12563..12913)
                     /gene="arsC"
                     /locus_tag="PLH85_06035"
     CDS             complement(12563..12913)
                     /gene="arsC"
                     /locus_tag="PLH85_06035"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765798.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNB64153.1"
                     /translation="MTDTIRLYHNARCSKSRSACALLADKGVPTTIIEYLKTPPTREE
                     LAAVLAALGMRAEDLVRKGEDVFKATFKGRTLSEDEWLDALVAHPILIERPIAVRGAR
                     AVVGRPPEKVLELL"
     gene            complement(12928..13863)
                     /gene="rpoS"
                     /locus_tag="PLH85_06040"
     CDS             complement(12928..13863)
                     /gene="rpoS"
                     /locus_tag="PLH85_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HNB64154.1"
                     /translation="MYEPGVPEEELETPEPDLPPEVEVFLETPAPVDTEFLGDVTQLY
                     LNEIGANPLLTAEEELSLSRRVREGDFPARQTMIERNLRLVVNIAKHYLNRGIPLLDL
                     VEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELNQ
                     VLRTQRQLEASGHGDTTVEEVARQLSRPVEDVRAILALNEHTASLDAPLDIDPSLSIG
                     ESLADEGAETPDTQIHGLEVESLVREWIGQLNEKQRMVIRHRYGIDECEVQTLEELAD
                     SLDLTRERVRQIQLEALGQLRRIIKRRGVSKDVLF"
     gene            complement(14008..14991)
                     /locus_tag="PLH85_06045"
     CDS             complement(14008..14991)
                     /locus_tag="PLH85_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB64155.1"
                     /translation="MLSRLSLSLFLSALALAGCATKSPAPVADRAKAGATPPVRAETP
                     AAPAPVVARPGYYIVKPGDTLIRIALDHGQNYRDIAAWSGLENPNLIEVGQELRVKPP
                     ESGAVVKPVAPAGADVRPVAPSADGLRREPKGGKVPYSEQAWAQAQKPEAPVVPVKAD
                     AKAEPRTDARPDAKPDVKPETKPEAKPETADDKGKDAAKPAAAGDDRIEWSWPAGGKV
                     MAGFNDTSSKGIDLSGKPGDPVYAAAAGKVVYAGTGLRGYGKLLIVKHDNAYLSAYAH
                     NQQLLVKEGQSVSKGQKIAELGDTDSDRPKLHFEIRKQGKPVDPVKYLPSR"
     gene            complement(15009..15668)
                     /locus_tag="PLH85_06050"
     CDS             complement(15009..15668)
                     /locus_tag="PLH85_06050"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNB64156.1"
                     /translation="MSEVARVGVHGAQRARARMVERLREQGVRDERVLAALLHVPRHL
                     FVEEALASRAYEDTALPLGLQQTISQPFVVARMLELLLAGRESLGKTLEVGTGCGYQA
                     AVMASLASDVYSVERLRLLLDRARANLRHLRLPNVRLKHADGSVGLKDAAPFDTIIVA
                     AAAPKVPPALVEQLAEGGRLILPVGMAEQVLVLIERTVRGPKETRLSPVRFVPLLPGT
                     E"
     gene            complement(15665..16408)
                     /gene="surE"
                     /locus_tag="PLH85_06055"
     CDS             complement(15665..16408)
                     /gene="surE"
                     /locus_tag="PLH85_06055"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNB64157.1"
                     /translation="MRILLSNDDGYFAPGLQALASALGDVGEISVVAPERDRSGASNS
                     LTLDRPLSLRRAANGFHFVNGTPTDCVHLAVTGMLEQLPDIVVSGINHGANMGDDTIY
                     SGTVAAATEGYLLGVPAIAMSLVSKEASDFSAAARIARELVARFIRQPVRQPTLLNVN
                     VPDLPYDQIKGIRVTRLGKRHKAEPTIKSVTPRGETVYWVGAAGAAQDAGEGTDFHAV
                     AHGYVSVTPLQIDLTHAGQIAPVQDWLAS"
     gene            16678..17070
                     /locus_tag="PLH85_06060"
     CDS             16678..17070
                     /locus_tag="PLH85_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64158.1"
                     /translation="MMHRSVTHALIGLGALLAAACTTLPGLATAPAQSRDGLLADAKG
                     MTLYVFDKDAAGSGKSACNGPCANNWPPLVAAEGDKASGDWSVIVRDDGRRQWAFKGK
                     PVYTWVKDQKPGDTTGDGFNSVWHVARP"
     gene            17091..17591
                     /locus_tag="PLH85_06065"
     CDS             17091..17591
                     /locus_tag="PLH85_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB64159.1"
                     /translation="MSFRQAIVAEIPRLRRYARALTGDASRADDLVQDTLERALGKWR
                     LWRPGNLRAWLFTLMHNIFVNQLQESVLVISLDGDTHLDVPVRPRQQDNLELRDLDRA
                     LARLSPEQREVLLLVGLEELSYEDTARVIGVPVGTVMSRLARARDKLRLLLAGDERAL
                     HLKVVK"
     gene            17588..18367
                     /locus_tag="PLH85_06070"
     CDS             17588..18367
                     /locus_tag="PLH85_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNB64160.1"
                     /translation="MNTPPDRDRLHAFADDRLPADERVRVAGWLADHPDSAAAVHDWR
                     RQNAALHAAYDGVLEQSVPPHLLAPPTPTPRPLLRVAAVLGWLALGIVIGRVLPPDGP
                     AATVDDPLAALPHRAAVAYAVYVPEVRHPVEVGADQESHLVSWLSKRLGTPLRAPHLV
                     AEGFRLVGGRLIPSEQGPGALFMYEDGTGARLTLYVVTVEHDGGETAFRFAHAGALST
                     FYWVDRHTAYALSGELPRASLLPVASAVYRQLGEDRPPAQK"
     gene            18439..19365
                     /gene="msrP"
                     /locus_tag="PLH85_06075"
     CDS             18439..19365
                     /gene="msrP"
                     /locus_tag="PLH85_06075"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNB64161.1"
                     /translation="MLIRRPADLPPSEITDPAVYGARRRFMGQAGALALGSLLPAAGQ
                     AALGPLGPLSHSALSTTETPTSLRAVTGYNNFYELGTSKTDPAENTFRLKPAPWTLRV
                     EGLVNKPRTFALEDLFRLAPLEERIYRMRCVEGWSMVIPWVGLPLASVLKLADPQGSA
                     KYVEFTSHYDRATMTRGVLDWPYSEGLRLDEAMHPLTILAVGLYGEVLPVQNGAPVRL
                     VVPWKYGFKGAKSLVSLRLVDKPPLTAWMKAGPSEYGFYANVNPDVDHPRWSQATERR
                     IGEFLKRKTLMFNGYADQVASLYTGMDLRRYF"
     gene            19372..20040
                     /locus_tag="PLH85_06080"
     CDS             19372..20040
                     /locus_tag="PLH85_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNB64162.1"
                     /translation="MNPDTRQLALLKTALFALCLVPAVLLGRAFSLDGLGANPIEALT
                     RATGEWTLRLLLITLCVTPLRKLTGWHWLVRLRRMLGLFTFAYACTHLLTYLWLDQFF
                     DWHAIAKDILKRPFITVGMAAFVLMAPLAATSSNAAIRRLGGRRWQALHRSIYPIAIL
                     ACVHFWVLVKKDITEPLLYALITAVLLGLRAWWREQERRRQLAGAYAPPPQARGKVIP
                     IVAK"
     gene            20090..20692
                     /locus_tag="PLH85_06085"
     CDS             20090..20692
                     /locus_tag="PLH85_06085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB64163.1"
                     /translation="MSLDHFAHKADSFDANPVRVDNVDAIARVLRDTVALDPAMRLLD
                     FGSGTGLLLERLAPHVAHITAVDVSPAMNRQLDAKRAHLPCTVEILELDLVAHDLAQT
                     FDGIVSSMTLHHVADVAALLRKFHGLLPDGGFIALADLDTEDGSFHSEDTGVFHAGFE
                     RAYIAGLAAAAGFREVQVHSASVVRKPQGDYPVFLLTAVR"
     gene            complement(20709..21161)
                     /locus_tag="PLH85_06090"
     CDS             complement(20709..21161)
                     /locus_tag="PLH85_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNB64164.1"
                     /translation="MSDLSFQHSLFARLRALEESAFPKTCANCGRKYRTHEEFLTATH
                     PVRRDHTGLKQSYDDDGAVIVDVFRNCVCGSTLLESFHDRRDTSPAGQKRRARFAEML
                     SSLVAAGLSESVVRAELLLLMRGKPNRLVELIRSVKDGAGGGDGLPLP"
     gene            complement(21222..21809)
                     /gene="mog"
                     /locus_tag="PLH85_06095"
     CDS             complement(21222..21809)
                     /gene="mog"
                     /locus_tag="PLH85_06095"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNB64165.1"
                     /translation="MSETAIIGLVSISDRASEGVYQDKGIPALQDWLGSALASPWRAE
                     TRLIPDERAQIEATLIELCDGVGCDLVLTTGGTGPALRDVTPEATLAVGHKEMPGFGE
                     QMRQVSLAFVPTAILSRQVAVIRGKTLIINLPGQPKAIAETLEGLKDGEGRPTVHGIF
                     AAVPYCIDLIGGAYIETHDAVIKAFRPKSAIRPKA"
     gene            complement(21806..22354)
                     /gene="yjgA"
                     /locus_tag="PLH85_06100"
     CDS             complement(21806..22354)
                     /gene="yjgA"
                     /locus_tag="PLH85_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNB64166.1"
                     /translation="MAHPHSHHDDDEDTGPSKSQLKRDSQALQELGQELAALGKERLA
                     KVPLDEDLRDAVRDYQRFPSHEAKRRQLQYIGKLMRHVDPAPIRAALDAFKGVSAEEN
                     ARMHRLEALRTRLLDDEKTLHDIATAHPGADLQHLRVLRRNALKERELGKPPRAFREL
                     FRVLRELEGAPDNDPPSGEDEA"
     gene            22424..23764
                     /gene="pmbA"
                     /locus_tag="PLH85_06105"
     CDS             22424..23764
                     /gene="pmbA"
                     /locus_tag="PLH85_06105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNB64167.1"
                     /translation="MAEKRFSFTQDELRTLADDILKYARKGGASACEVDVSEGFGQSV
                     TVRRDEVDTIEYNRDKGIGVTVYDGQRRGYASTSDFSAASLKATVAAALSIARFTAPD
                     PAAGLPDAALLARDIPDFDLYHPWALSSEDAIELARRCERAAFAVSPEIRNSDGASVS
                     MQESHFISANSLGFMGGYSSSRHFLSCSVIAGEGDDMQRDDWYSSKRDPAELADAEEV
                     GTFAARRALARLGARQIPTCEVPVLFEAPLAAGLIGAFVHAVSGGALYRKSSFLLDSL
                     GKPVFPGHIQISERPHLPKAFASSAFDDDGVATRDREVIVDGVLQGYFLSTYSARKLG
                     LETTGNAGGSHNLLVKPGAHDLDGLVRQMDRGLLVTELLGHGVNYVTGDYSRGAAGFW
                     VENGQIQFPVHEITIAGNLKAMLAGIREIGSDVLVRGSKHCGSILVDRMTVAGA"
     gene            23802..24908
                     /locus_tag="PLH85_06110"
     CDS             23802..24908
                     /locus_tag="PLH85_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNB64168.1"
                     /translation="MERRRFLTQAGAGMAAGALAAPAAAAMADLPILKWRLASSFPKS
                     LDSIHSSTESLARQVALLTGGRFQIQVFAAGELVPSAAVLDAVKDGTVEIGHSASYYY
                     VGKDPTFALDTGIPFGLNSRQQMAWMHDGGGLELLRDFFREYAIHNIPCGATGTQMGG
                     WFRKEIRSVEDLKGLKFRVGGFAGQVLARLGVVPQQIPAGDIYAALEKGAIDAAEWVG
                     PYDDLKLGFQRVAKYYYYPGFWEGGPQLTVYVNLKHWNALPPEYQAALENACALAHLE
                     MQARYDARSPAALRQLIASGTQLRPFSHEIMSAAYQAAHALYDETAAKNPRFRKIYEP
                     WKKFRDDQLQWFAVAESHFDAFMQATRAAARSRK"
     gene            25006..26109
                     /locus_tag="PLH85_06115"
     CDS             25006..26109
                     /locus_tag="PLH85_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNB64169.1"
                     /translation="MERRSFLKKASAGVAAGAVAAPALAAELPTIKWRLASSFPKSLD
                     TIFGGADVVAKRVAAATGGKFQIQVFAAGEIVPGPGVMDAVKDGTVECGHTCSYYFVG
                     KDPTFALDTAIPFGLNSRQQTAWMMDGGGLELFREFFKDYNIYNIPCGNTGAQMGGWF
                     RKEIKSVEDLKGLKFRCGGFAGQVLARLGVVPQQIPGGDIYPALEKGTIDAAEWIGPY
                     DDQKLGFNKVAKYYYYPGFWEGGPQISLYVNMKQWAALPKEYQTILEDACLYAHAEMQ
                     ARYDAKNPGALKQLVGSGTQLRPFPNDVLAAAYKAANDLYDETAASNPKFKKIYEAWK
                     KFRDDQLQWFAVAENRFDNFMQAARNASAKAKK"
     gene            26183..26539
                     /locus_tag="PLH85_06120"
     CDS             26183..26539
                     /locus_tag="PLH85_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNB64170.1"
                     /translation="MIAAFALLLGFQLAGETLRVALHLPVPGPVMGMALLLVYLIFRG
                     GPDDGLRTTSGGLLQHLSLLFVPAGTGVMLHAGRLADEAGPILVALVASTVLGLAATG
                     LTLHALTRRSRQEDAQ"
     gene            26536..27270
                     /locus_tag="PLH85_06125"
     CDS             26536..27270
                     /locus_tag="PLH85_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNB64171.1"
                     /translation="MSSGMTEIWVYLSAQPLLWLSLTLAAYLVALTLHRKAGAHPAVN
                     PVLLSVLILVGLLVATGTPYQHYFDGAQFVHFLLGPTTVALAVPLHAQLKRLVAMGGP
                     LLIALAVGSLTAGLSAYAIGALLGASTPTVISLAPKSVTTPIAMGIAERLGGLASLTA
                     VFVILTGIFGAIFATGLLHRLGVKDEASTGFALGLASHGIGTARAFQLSEQTGAFAAL
                     AMGLNGLFTALTLPWLLPLLAPLLSR"
     gene            27274..28215
                     /locus_tag="PLH85_06130"
     CDS             27274..28215
                     /locus_tag="PLH85_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNB64172.1"
                     /translation="MDLLIDVILKAGRSAVELSFFVLLPVMVVMLSLMRLLEARGVLD
                     WLVEKLAPALRPLGLTGLGVFAALQINFVSFAAPVATLAMMDQRGASSRHLAATLAMV
                     MAMAQANVSMPMAAMGLHFGIVLGWSLVGGLVAAAATYYGFGRHLSAVEHTLDETLNH
                     PVAESAKGVLDVINRAGAEAFKIAVGAIPMLVLSLTVVLALRRLGALDVLTGLLAPIL
                     LAVGADPALILPTLTKYLAGGTAMMGVMDEMLRAGTATAATLNGASAGLLIHPLDIPG
                     VAVLISAGRRVADVWKPAALGAGVGILARVAGHLVVG"
     gene            28416..29147
                     /locus_tag="PLH85_06135"
     CDS             28416..29147
                     /locus_tag="PLH85_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNB64173.1"
                     /translation="MMKLLDVLAEYSDPVPLKQLALETGLHPSTAHRILGAMSGSGFV
                     ERSEAGAYRLGIRLLELGSLVKSRISLRETAMPAMLRLHAETGESVNLGIRAGDEIVY
                     VERTSSGRSSVRVVHIVGARAPLHTTATGKLFLVEDGAHQVKEYARRTGLPGTTPTSI
                     TTLAALEKELDKVRRHGVAYDLDEVENGVRCIAAGIRDDSGELVAGLSLSTPAERFNP
                     DWAPLIKATADEISRALGYVPPAGR"
     gene            complement(29211..30236)
                     /gene="pbpG"
                     /locus_tag="PLH85_06140"
     CDS             complement(29211..30236)
                     /gene="pbpG"
                     /locus_tag="PLH85_06140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HNB64174.1"
                     /translation="MNIRKSLLALAGAVALQATGPADALAATHRPAKKSFVAKAAPAP
                     AKNAKARNRAAARAAFASPQVQRTAALAVAPEMDHEGNPVLASAAFMVADQATGTVLL
                     EKNSDAVLPIASITKLMTAMVVLDARLNLGEVLTVANDDVDTLRGSSSHLPVGVDLTR
                     EDMLRLALMSSENRAASALARHYPGGLPAFVAAMNRKAVSLGLKDTRFSDSTGLNSAN
                     VSSARDLVKMVSAASRYPLIREFSTTGNYTVYLNGRPRQFGNTNALVKSPDWQIGVSK
                     TGFINESGKCLVMQAWLLNKPMIIVLLDSFGRFTRVADAQRVKRWLESAAAQQRLAEV
                     VRGGPTT"
     gene            complement(30454..31611)
                     /locus_tag="PLH85_06145"
     CDS             complement(30454..31611)
                     /locus_tag="PLH85_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB64175.1"
                     /translation="MTDGRTALVLTGGGARAAYQVGVLVAIRDVWGKRSGNPFPILCG
                     TSAGAVNAVALAAYSADFDAAVRKLAFIWRNFHVDQVYRADTAAVVRSVTRWGSAIFL
                     GWLFHQVPRSLLDNEPLRALLTAMLDFGAIDRAIADGHLRAVSVTASGYTSGESLSFF
                     QGVPALEPWRRAQRVGLRASLAVDHLLASSAIPFVFPAVRLHREYFGDGSMRQQAPVS
                     PAIHLGARRILVIGAGRRGEEARQRTYDYPSPAQVAGHAMASIFLDGLTTDLERLERI
                     NATLAALPPGAREAAGIPLRPIETLVIAPSQRLEYIAARYRNALPPALRLVLRGVGAT
                     RRNGSMLLSYLLFERSYTRALVELGYRDAMARRGEIARFLGLDSVQGRSSD"
     gene            complement(31766..34033)
                     /locus_tag="PLH85_06150"
     CDS             complement(31766..34033)
                     /locus_tag="PLH85_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNB64176.1"
                     /translation="MDERIRLAALEYHRLPKPGKISIAPTKALTNQQDLSLAYSPGVA
                     AACDAIVEDPAEAASLTARSNLIGVVTNGTAVLGLGNIGPLAAKPVMEGKGVLFKKFA
                     GIDVFDLEIDEPDADKLIDMIAALEPTFGGINLEDIKAPECFYIEQKLRERMKIPVFH
                     DDQHGTAIVVGAAFLNGLHLLGKDLKQVKLVTSGAGAAALACLDLLVMLGVPVENIWV
                     TDIEGVVYEGRTVLMDPIKARYAKTTSARTLGEVIEGADVFLGLSAGGVLKPAMVAKM
                     ASRPLIMALANPTPEILPEEVRKVRDDALMATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTITDEMKLAAVKAIAELARAEASDIVAAAYGQEVSGFGPEYIIPKPFDPRLIVKIA
                     PEVARAAMASGVATRPIADFDAYCQQLNNFVWQSGLVMKPVFQAAKRSPKRIIFAEGE
                     SERVLRAVQTVVDEGLCRPVLIGRPDVTLENIDRYGLRLRPELDFELVNPETDPRYDQ
                     LWTHYHALMERKGVSVDYAKREVRRRPTLVGALLLKFGYADGLICGTYGMHGLHLRFI
                     ETVLGRQTGVRNFYTMNLLSLPGRTVFLCDTYVNYDPTPEQVVEMTRLAADEVRRFGI
                     QPRIALLSHSSFGTDNSPTAEKMRKALVMLHAEHADLEVEGEMHGDAALDASIRQQIF
                     PNARMREDANLLIFPTLDAANIAFNLLKTAAGEGMTVGPILLGSAKPVHILTPTATVR
                     RIVNMTALTVVEAGR"
     gene            34317..36173
                     /locus_tag="PLH85_06155"
     CDS             34317..36173
                     /locus_tag="PLH85_06155"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNB64177.1"
                     /translation="MSQSIAEAAIAAAPACVKNEKLKQWVGEIAALTEPDRVVWCDGS
                     QEEYDRLCAEMVDAGMLIKLNPEKRKNSYLAWSDPSDVARVEDRTFICSQNKGDAGPT
                     NNWEDPAKMRKTLNELFKGCMHGRTMYVIPFSMGPLGSPIAHIGVEISDSPYVVTNMR
                     TMTRMGKAVYDVLGADGEFVPCVHTVGAPLGQGEKDSRWPCNPDTKYIVHYPETREIW
                     SYGSGYGGNALLGKKCFALRIASNMARDEGWLAEHMLILGVESPQGEKTYVAAAFPSA
                     CGKTNFAMLIPPRSFDGWKIYTVGDDIAWIKPRTDADGVTRFYAINPEAGYFGVAPGT
                     SEKTNFNAMATLKENIIFTNVALTDDGDVWWEGMSKEAPAHLIDWQGKDWTPELAKET
                     GRKAAHPNARFTAPAAQCPSIDPNWENPEGVPISAFIFGGRRATTVPLVYQAFNWNFG
                     VYMAATLGSETTAAAFGAQGVVRRDPFAMLPFCGYHMGDYFNHWLKMGHAVDQTPGIF
                     CVNWFRMNEKGEFMWPGFGENMRVLKWIVDRVRGRVAAQENALGWMPQYEDIDWTGSD
                     VTKAEFEALTAIDADAWKTELASHKEWFDKLQDRLPKQFVLKRELFELALSV"
     gene            36256..37416
                     /locus_tag="PLH85_06160"
     CDS             36256..37416
                     /locus_tag="PLH85_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB64178.1"
                     /translation="MTRFARRMADIQPFHVMEILKRAHELAAQGRDIIHLEVGEPDFP
                     SPKPVVEAARCFLNGGHVHYTPALGLSALREAIAGFYRDRFGADVAPERIIVTPGASG
                     ALMLALAVLTDPGDAWLLPDPGYPSNRHLVRSFEGVPVALPVDATTRFQPTPTQVAEA
                     WTTQTRGLMVATPANPTGTLLTPGEIVALHAAVTARHGVLIVDEIYQGLCYGVDATTA
                     LTRLDDVFVVNSFSKYFGMTGWRLGWLVAPPDYVRPLEKLAQHFFIAPSTPAQHAALA
                     AFRPDTLAILEARREAFAQRRNTLLPALQTLGFRFATEPQGAFYLYADITHLADDSQQ
                     LARRLIEEAGVATTPGIDFGDHGPERHLRIAYTTDENRLLEAAARIRRVLGL"
BASE COUNT         6071 a        13379 c        11821 g         6267 t
ORIGIN      
        1 ggccaagcgg gaagagagcc gcttaagagt ctcgtcaggc cgcatcatcc aaccgttttt
       61 tctatggtgg gtcggatgat gggtagaaaa gaaaaacgcc ccaaaatggg cgtatttgct
      121 aagtatctgg cggaagcggt gagattcgaa ctcacgaacg gttgcccgtt gccggttttc
      181 aagaccggtg caatcgacca ctctgccacg cttccggaag gccctgatcg acggcgggat
      241 gccgtcaggc cggcccgaat tctagcacgg ccccccaggc gcgttctccc tgcacggttg
      301 aaactttccc gcacgtccga tactctcagg cagtacagcc cctcaacggg cggcgacctt
      361 aaccctctca attcaatgga gaaatctatg caaatcgaca cttacggcca gcagacgcag
      421 atgcttggcg ccagtacgaa ccgcgtgctg cgcaatacat acctgctgct tgcactgtcc
      481 atggtgccga ccatcgctgg cgccatgctt ggcgtgcaga tgcagttctc gttctttgcc
      541 ggcagcccgc tgatgggttt cctgctgttc atgggcatct ccttcgcctt cttctggggc
      601 atcgagcgca acaagaacag tggcctgggc gtggcgctgc tgctgggctt caccttcttc
      661 atggggctga tgctgtccag cctgctgcgg gtggccctcg gcttctccaa cggcggcacg
      721 ctgatcgccc tggcgggcgg cggcacggcg gcgatcttcg gcgtgatggc caccgtggcc
      781 acggtctcca agcgggattt cagcggcctc ggcaagttcc tgttcgtcgg cgtgatcgtc
      841 ctgctgctgg ccagtgtggc caacatcttt ttccaggtgc cggccctcta cctgacgatc
      901 tcggcgatcg cggtggcgct gttctccgcc tacatcctgt acgacatcaa cgacatcgtg
      961 cgcggcggcg aaaccaacta tgtgacggcc accctgcggg tctatctgga cgtctataac
     1021 gtgttcgtca atctgctcaa cctgctcatg gccttcggtg gcgaccggga ctgatcccac
     1081 cccgtccggc aaaaaggcga cccgcgggtc gcctttttgc tgacccgtgc ctcggctaat
     1141 cccgctcgaa cagggcgatg gactccacgt gggaggtctg cggaaacatg ttggcgatgc
     1201 ccgccccctt cagccggtag cctttctcgt gcaccagcac cgccgcatcc cgcgcgaggg
     1261 tcgcgggatt gcaggacacg tacacgacgc gccgcggact gtccgcgccg agcgccttca
     1321 ccaccgcaat agccccttcc cgcggcggat cgatgagcag cttgtcgagc cgaccgaggg
     1381 ccgcgagact gtcttcggtg gcttcgaaca ggttggccgc atggaattcg ctgcgttccg
     1441 ccaagccgtt ggccgccgcg ttctcagcgg cgcgccgcac cagcgcgtcg ctgccttcca
     1501 ccccgaccac tgtggccccc aggcgggcga tgggcagact gaagttgccc agcccacaga
     1561 acaggtccgc aatgcgctca cccggccggg ggtccagcaa gagcatggcg cggcgcatca
     1621 gcaggcggtt gatgcccatg ttgacctggg tgaaatccgt cggccggaaa gccatggaga
     1681 cgccgaactc cggcagcgtg taggccagtc cgggcgcctt gaccgggtgc aggcgagagg
     1741 ccgaatccgg cccgaagggc tgcagccaca cctgcacgcc gtgggattcg gcaaacgccc
     1801 gcaggtggtc cttgtccgcc gcggtcaggg gttccagatg gcggaatacc agcaccgtga
     1861 ggccctcgcc caccgccacc tcgatctgcg gcagccgatc cgccaccgac agggccgtca
     1921 ccagatcccg cagggccggc agcaggaccg acacatgggt cggcaacacg ggacagctgc
     1981 gcatatccac aatgtgactg ctgcgccgtt gatggaagcc gatccgcacg ccgcccttgc
     2041 taggcacctg gcgcacgctc agacgcgcgc ggaagcggta accccatggc gcgcccgaaa
     2101 tgaccggata cagcacgtca gccttcaccc gcgccagatg ccacagggcg ttctccagca
     2161 cccgctgctt ggccgccagc tgcgcagagg gttcaaggtg ctgcatgctg cacccgccgc
     2221 actcaccgaa caggacgcag ggcggtgcca cgcgttgggc gctgggtgcc acgatgcgcg
     2281 tcacgtctgc caattcgaac ttgggcttga cccggtagga gacgaactcc acccgctcac
     2341 cgggcagtgc gccttcgatg aagaccgtct tcccgtccac ccgcgcgaca ccgcgcccat
     2401 cgtggtccag ggattcgatc agcgcttcgc ggggggcgtc gacatgcatg ggcgcaagag
     2461 tgaaaagaaa ggtcgccatt ctagcgacag agccggctgt tgcaaggcaa gggcgtataa
     2521 tcacggcatg atcgacacac ttctgggtgg cctttcggtc gagcagttcc tcgccgaata
     2581 ctggcagaaa aagcccctcc tcatccgtca ggccatcccc ggcttcaccg gcgtggtctc
     2641 cagggacgac ctcttcgccc tcgcccgcga tccagatgtg gaatcgcgct tcatcgccca
     2701 cgacggtgcc cgctgggaag tcctccacgg cccgcaaaaa gccaccgacc tgcgccgcaa
     2761 gaagcaaccg tggacggtgc tcgtgcaggg actcaacctc ttcgtgcccg aaggcgacgc
     2821 cctgctccat cgtttcgact tcatccccca ggcccgcctc gacgatctga tggtgagcta
     2881 cgccgtcgat ggcggtggcg tcggacctca ctttgacaac tacgacgtgt tccttctaca
     2941 gggcatcggt cagcggcgct ggctcatcag caaccaggac gaccgcagcc tcatcgagga
     3001 tgtccccctc aagatcctgc aggatttccg gccggtgcac gactgggtgc tggaatccgg
     3061 cgacatgctc tacctgccgc cccactgggc ccacaacggc attgcccttg gagaatgcac
     3121 cacgtattcc atcggcttcc gttcgcccac ctaccaggag ctggcccagg agttcctggt
     3181 ctatctccag gatgccgtgc agctcgaagg cctttacgcc gaccctgacc tgaagcctca
     3241 cgtccactcc gccgagatcg gcgatgccat ggtggatcgg gtggccgaac agctcgcgcg
     3301 gatcacgtgg acacgggagg acgtgcgccg tttcctcggc gcctacctga ccgaacccaa
     3361 gcctcatgtg ttcttcgact cgccggacga accactcgat cacgacagct ttctcgccca
     3421 gtgcaacagg gcgggctatg tgctggacgc gcgcagcctg cttctgttct cggacggccg
     3481 cttctttctc aatggtgaag ccgtgagcat ctcccacacg gatctgcccg tcatgatcga
     3541 actggcagac cggcgcagga tctccacggt tgccgatctg acgcaggatg gacaggccct
     3601 tctgtacgac tggtactgct gcggctttgc ccacccggac agcatcgaaa gcaaataact
     3661 tcaagcactt ggcatctacc gcgacattcc atcgatgttg cggcaacgca acaatgtctt
     3721 tctggggcca cacccggcgt gctagaatcc tctcagatcg ggtcccccac ctgattgctc
     3781 ctgattttca ggccgttgaa ctcacttatc gataagggaa accaacaatg aaacagtctc
     3841 tcctcgttgc tgctctcgtc gccctggccg tttccgcttg cggcaagaag gaagaaccca
     3901 aggccgttga agcgccggct cctgccgctg ctccggctcc cgctgctgct ccggccccgg
     3961 ctgccccggc tgctgacgcc aagcctgctg aagccgctgc cccggccgct cccgctgccc
     4021 cggctgctga cgccaagccc gctgaagctg ctcccgccgc tccggctgcc gacaagaagt
     4081 aatccttctt ccggcaacga aaaaagccaa cctgcgggtt ggcttttttt gtacccattg
     4141 atggagcaca tcgcggtctc catccctctg ccacaccgcg aggcacctcc ccgcaggacc
     4201 gtccgcccat cgcgcctgtc ctgaatcggc aagcctggga tcggataccg cagccgccgc
     4261 ttcaagaaaa acgccgaccc tcaggtcggc gttcatccgt gacgggtgcg caggccttac
     4321 ttgagctgct cgtacagcaa gcccagaatc ttgcgcgccg aatccgattt gtcgacaccc
     4381 ccttcgcggg tgaggacctg aaccgacgat tcgtctcccg cattcttgac gaagatgcgg
     4441 tactgactgc ccgtcggcag tgccggcttg tcgctgcgcc agaaggccag tttggagagc
     4501 aagccctcgt cacccttctt tttgacgtcc acttcagggt cgacatagcg cacgtagtac
     4561 agaccctggg agcgatcccg gtcctcgacg gtgaaaccga cccgatccag tgccagcccg
     4621 acacggcgcc aggcgcggtc gaaggcatcc tgcacggtca gcagcgttgc cccatccgcc
     4681 gcattggcca gcctggcctt gtccgaagac ggcgcagtgg ccagttgggc cttggctcgg
     4741 ctttcgtccg caccgaggcg gaccatcagg cgctgcagga actccgcttc aagttccgga
     4801 tcggccgctc ggggctgcca gatcgtgcgg tccttgcctt ccgtaggata gatttccatc
     4861 atgccccggt ggctgatgta gatctccacc gtgcccgctt cggcaccggc ttccagacgg
     4921 gtgcggaact tgtcgcgctc cggtgtggaa tacaggccat cgagcacctt gccaagcgtg
     4981 ttgcggatga agccctgctc gatcttcgcc cggttctcgg cccagtcggt ttccattacg
     5041 ccgatttcag gcttgtccac gttcaggagg aaaccgcttt cctgccagaa atcgcggatc
     5101 tgcggccaca gcttgtcggc tgaaccctgg accaccagcc agcgctggga accggcacgc
     5161 tcgatatgca tcttgtcgga ccccgacgca gatgccgcgg tcggcgctgc gccaggcgtg
     5221 gcgcccacct gtgcgccctg gcccgtgcgt tcggcactgt aggccgagaa cgtggcggtt
     5281 cccttgggac tgacatcggg caccgtgtaa cggtcatcgc gcgtcggggc ggtgagatcc
     5341 ggcggtattt cgagggactt gaccggcgca gcggacgcgc tcttgtagtc gatcttcttc
     5401 gattccagca gggaaccgga gcagccggcg agggcgatcg ccatcgcaac cgccgatccg
     5461 gacgccaggg tgcggctatg catgcgtaag cctcgggagg ggatggtgga actcaaactt
     5521 cgatgccggc ctggcgcatg gccttgcgga cacgctcgtg gcaggattct gacagccggg
     5581 tgaggggcag gcggatgccc ggaccgatca gccccatctg ctcgacagcc cacttcacgg
     5641 gaatcgggtt ggcttcgcag aacaggtcgc ggtgcagacc gaccagcttg gcgttggccg
     5701 cacgggccgt gacggcgtcg ccctggagcg ccgcggcaca cacctcatgc atcagccccg
     5761 gcgccacgtt ggcggtcacc gaaatcgtgc cgtgcccacc cagcaggatg aaggccatca
     5821 tggtcatgtc gtcgccactg tacagggcga aatccctggg tgcccgggcg atcagatcgc
     5881 aggcgcggtc cagactgccg gtcgcatcct tgatgccgat gatgttggga atctcggcaa
     5941 gccgcagggc cgtgtcattg gccagatcag ccacggtccg gcccggcacg ttgtagagga
     6001 tcagcggcag ctcgctcgcc tcggcgatgg cccggaaatg gcggtacagc ccttcctggg
     6061 tcggccggtt gtaatagggc accacggaca ggcccgcgac agcgcccgcc ttgcgggaga
     6121 actcggtcag ttcgatggcc tctcgggtcg aattggcccc cgtgccagca atgaccggaa
     6181 tgcggcccgc cgcatgttcc acgcagacac ggatcagttc gcagtgctcg tccacatcaa
     6241 cagtgggcga ctcaccggtc gtgccgacca cgacgatgcc gtccgttcct tcctggacat
     6301 ggaagtcgat cagcgtgcgc agacggggaa gatccaggct gccatcgtcg ttcatggggg
     6361 tgacgatggc gacaatcgaa ccggtaatca tgagctatcg cgggaaaggg aaagtgaact
     6421 attgtaccca atcacttggg gaaggccagt gggcgaaaaa tgcgcttacc aaaagcccgt
     6481 ctgcgcagcg cgaaccggat gccagaagcg cgggcagcgg aacgcccggc cggatcggac
     6541 cctccggccg ggcgctccgc tactcagagg tcagccaatg acttgacgtg cgccacaacg
     6601 ctgcgcgcca gggaagacag ggaatagccg ccttccagca tggagacgat gcgccgcttg
     6661 gagaactcgg ccgcgacctt cttgagctct tcggtcgcga agatgtaatc cttctccacc
     6721 agcccgaggg agcccatgtc gtcctcgtag tgggcgtcga aacccgccga gatgaagatc
     6781 gcctccggcg cgtggttgcg cagggccggt acccagatgt tggtgacgat ctcgcggaac
     6841 acgtcgccgc gggaacccgc cttgacgggc acattgacca tgttgggcgc gggattgtca
     6901 tgtccgctgt aggggtagaa cggatgctgg aagatgctga ccatcagcac ccgcggatcg
     6961 tccttgaaga tgtcctcggt gccattaccg tggtgcacgt cgaagtccac caccgccacg
     7021 cgcttgagtc catgcacatc gagggcatgg cgggccgcaa cggccacatt gttgaagaag
     7081 cagaagccca tggccttggc cgcttcggcg tggtgaccgg gcgggcggac cgcacagaag
     7141 gcgttctcca cctcgccgga catcaccaga tcggtcgcca tcacgccggc accggccgaa
     7201 cgcagggccg ccttgatgga gtgcgggcac acagccgtat ccggatccag gtgacggatg
     7261 ccatgctccg gcgcgctggc aaggattgcg tccacgtgct cgcggggatg ggcgcggctg
     7321 agctgttcgg cggtggccac cggcgcgtcg tagaacgaga ggtacatgtc cagccccgcc
     7381 gcgatcaggc ggtcgttgat ggctgcgaga cgctccgggg attccggatg atgggcgccc
     7441 atgttgtgca gccagcactc ccgatgcgtg atgaatgcgg tactcgtcat acactctccc
     7501 cctctactcg cgaaaacgtc tgtttgattg attgatcgtc ttcccggcga acttcacagc
     7561 cccatgcggc tccatctcct ggagaccacc cgcagcaccg ggaatgcact ggtgatcccc
     7621 attatccaca taacccacgc cctgcaaaag cccacgccat gcccctttac atcgccaatc
     7681 gcctcgtgga tgccgccggc cggattctcc tcggcaagga gcaggccatc cgcctcgccc
     7741 tggcctgcct gctggcccgc ggccacctcc tgatcgagga tgttccgggc gtcggcaaga
     7801 ccaccctggc ccacctcctt gcccgcctgc tggggctcca gttccagcgc atccagttca
     7861 ccagtgacct gctgccggcc gacatcctcg gcgtgtcggt gttcgacccc cgcgacgggg
     7921 ccttccgctt ccatccgggc ccggtcttcg cgcagatgat cctcgccgac gagatcaacc
     7981 gggccacccc caaggcccag agcgccctgc tcgaagccat ggaggaaggc caggtcagcg
     8041 ccgagggccg cacgctgccc ctgccggccc ccttcttcgt catcgccacc cagaacccgg
     8101 ccgaccagat cggcaccttt ccgctgcccg agagccagct cgatcgcttc ctgatgtgca
     8161 tcgccctcgg ctaccccgac ccggccgcag agcgggccct gctggccggc gaggatcgcc
     8221 gcgcgctgct ggcccgccag gagacactgc tcgcgccggg cgagctggac accctgttcg
     8281 acgccgcagc ccgcgtccat gtggcccccc cgctgctcga ctatgtccag gccctgctcg
     8341 cccacagccg ccaggccacc ggcttcagcc tgggcctgtc gccccgcgcc ggcctgggtc
     8401 tggtggcctc ggcgcgggcc tgggcgctgc tggccggacg tgaccacgtg cagcccgagg
     8461 acgtacaggc cgtcctcccg gcggtggccg gccaccgcct gcgcagtgac ggggaccgcc
     8521 gcacatcgcc tgaagaacgg gtccgccgcc tcctcgaagc cgttcctctg ccctgatgcc
     8581 ggtccacgcg cgccttgacg cactgcgggc ccgcttccag cgctggctgt tccgcgtcca
     8641 cggcccggag ccggcgccga tccgcctggg ccagcgccgg atcttcgtga tgcccaccgc
     8701 ctttggcgtg gcgctgggcg tgaccctgct ggtgatgctc ctcgcctcca tcaactacgc
     8761 cctgagcctg ggctacgccc ttaccttcct gctgggcggc gtgggctggg tccacatcca
     8821 cctgagcttt cggaatctag tgggactgtc aatagcgccg ggacgggtcg agccggtctt
     8881 tgccggcgcc acggccagtt ttggcgtcct catgaccaac ccgctgccac gttcccgtct
     8941 cgccctgcgc ctgtggccag cccgcacggg cgcgtccccg gacgaagcct tcgagctccc
     9001 gcccgacagc caggtgcgta taagcgtcca cgtggcggcc ccccaacgcg gctggctgcc
     9061 cctcccccgc ctccagctgg agagccgtca tccgctgggt ctcatccgcg cctggagcct
     9121 gttccagccc gatcttcgcg ccctggtctt cccgacgccc gaagccgacc cgccgccctt
     9181 gccgcgcggc acgggtctgc ggccgggcgc ccacggcaga cgccaagggc gtgacgactt
     9241 cgccggcctg cgcgcccacc agcccgccga cccgccccgt cacgtggcct ggaaagccct
     9301 tgcccgcagc ggcgaatggc tcaccaagca gtttgacggc gggggcgcgg aggcgctctg
     9361 gctggactgg aacaccctgc cccccggtct ggacacggag cagcgcctcg cccgcctgac
     9421 ccgctggctg tgcgatgcgg aggccgcggg actggagtac ggcctgcgcc tgcctcagca
     9481 aacctttccg ccggccagtg gtcccgccca tcgccttgcc tgcctcaccg cgctggccct
     9541 gttcggaggc cctgatggct gagcgtggcc tgcgtccgga ccagaccggc tggctcgtcg
     9601 tcgcagccgg cgtcagcctg ctgccccacg ccgcgcagct cccccactgg ctgtccgccg
     9661 tctgcgcgct gttcctgtca tggcgcggca tgctcgccgc gcagcgcgcg cgcctgcccc
     9721 accgggcgct gctcggaacc ctcggcctcg ccgtcgcggg cggggtgttg gtcgaatacg
     9781 gccacctgtt cggcaaggat ccgggcatcg ccctgctggc cgccttcctg ggcctcaagc
     9841 tgctggagac ggacgacatt cgcggcgggc gcgccgtgat cctgctcacc tttttcctgc
     9901 agatgggcca gttcctgcac cagcagggca tggccaccgc cgccctggcc ctggtcggca
     9961 cggtcgtctc cctggccacg ctggcggccc tcgccggacg cgcccggccc ctgcgcccac
    10021 gcctgacaga agccgcccgc ctcaccgtcc tgagcctgcc cttcatggcc gtgctgttca
    10081 tcctctttcc gcgcgtccaa gggccgctgt gggggctccc ggcggacgcc ttcagcgcac
    10141 tgaccggcct ctccgacacg atgacgccgg gtgcccttgc acgtctgagt gaatccggcg
    10201 cgatcgcctt ccgggccacc ttcgacggcc cgccgccgcc ccgcgcccag cgctactggc
    10261 gcggcccagt gctgacccgc ttcgacggtc acacctggcg cgccgcccac ctgcccgccc
    10321 gggacgtgcc cgcgtacgcc gtttccggac gcgcctacac ctacaccctg acgctggaac
    10381 cccatgaccg gccgtggctg ctggccctgg actttccaga tgcccaggcg ggcctgcgct
    10441 acggcgcaga cttccagctc ctcgcccgtg agccggtccg cagccgcctg cgccagaccc
    10501 tggcgtccta cccagacacg ccggtcggtc tcgacgagcc ccccatcctg cgacgcctgg
    10561 ccctggccct gccggccggc gccaatccgc gcaccgtcgc cgcaggcagc cagctgcggg
    10621 ccaacgccgt ccgggcggac gacatccccg cgcgcggcat tgccctcatg cgcgacgccc
    10681 gcctcaccta cacgctggag ccgccccccc tgggtgagga cgacgtggac gccttcctgt
    10741 tcgacacccg ccaaggcttc tgcgaacact tcgccgccgc cttcgtggtg ctgatgcgcg
    10801 ccgccggcgt gccggcgcgg gtggtcaccg gttatcaggg cggcgagatc aacccggtgg
    10861 acggcagtct tgaagtccgc cagtccgacg cccacgcctg ggcggaagtg tggctcgccg
    10921 ggcgcggctg gctgcgggtc gaccccaccg ccgccagcgc cccggcgcgc atcgagcgcg
    10981 gcctcgccgc cgccctcccg gacaccgggc acctgcccct gctgatgcgc ccggagctgg
    11041 cctggctgca gggactccag caccacctgg acgccctcaa ccaccgctgg aaccaatggg
    11101 ttctcgggta caatcccgcc cgccagcgcg acctcctcgc cggtctgggc ctgggcgagg
    11161 tggactggcc tcagctcacc gggctgatga ccgccgcctg cgcggccgtc accgccgcac
    11221 tcgccggggt gctgttgcga aaccgccgcc gcgacccggt gttgcgtgcc tgggaaggcg
    11281 tctgcagccg cctcgccgcc cacggtctgc cccgcgcgga ctgggaaggc ccgacggcct
    11341 acgcggcccg cgtgggcacc acccgccccg atctggccga cgccatcgca gggctcgcta
    11401 cagattttgc gcgcctgcgc tacggcgagg cgtcagacac cgccgcccgt cgcgaccgat
    11461 tcctccattc cgcccgacgc ttccgccccc gatgattctc cgccccttcc gcctccgtcc
    11521 cgcactcctg ctggccagcg ccctcgccgc cgggccgctg gccgccgccg aacgctacgc
    11581 ggaccgtccc gacgtccagc aattcatcgc cgaaatgaag gaacgtcacg gcttcgatcc
    11641 ggatgccctc accttcatct ttcgtcgtgc cactttccag cccgccgtga tcaaggccat
    11701 ctcgccgccc aaggatcccg gcgtgcgctc ctggcagcgt taccgcggcc gcttcatcga
    11761 gccggtgcgc atcaaggccg gcgtgcgctt ttgggacgcc tacgcggaga caatcaaggc
    11821 cgccagtgac aagtacggcg tgccggagga gatcatcgtc ggcatcatcg gcgtggaaac
    11881 gatctacggg cgcaataccg gcagcttcca ggccgtctcg gccctgtcca cgctggcctt
    11941 cgactatccc cgccgggccg agctgttccg cggtgagctg gagtccctgc tgctgatggc
    12001 ccgcgaacag ggccgagacc ccctcgacta ccagggttcc tacgcgggcg ccctgggcct
    12061 gccccagttc ctgccgagca gcgtgcgcaa ttacgcggtg gattttgacg gcgatggcca
    12121 catcgacctg ctggccagcc cgaaggacgc catcggcagc gtcgcccgct acctgcagat
    12181 gcacggctgg gaacggggcg ggccggtggc catccgggcg gtcacgaatg ccgacgcgac
    12241 gctggaaacg ctgatcgcca acgacatcac gccggccttc gacgccgagg ccctggccgc
    12301 ccacggcgtg cgcgccgcct ccggaacgcc cgcggtcggg ccggccgcct tcgtggaact
    12361 ggtcaccccc ggcgccgaca gcgaatactg gctgggctat cagaatttct acgtgatcac
    12421 ccgctacaac cgcagcagct tctacgccat gtcggtgttc cagctggccg aggccgtcaa
    12481 ggaagcccgt caggcgcgcc gctgaggccc gcgcatcccg cccggaaccg gccgtccgac
    12541 cgttgcgacg gtcggacgtg cctcagagca gttccagcac cttttcaggc gggcgtccca
    12601 ccacggctcg cgcgccgcgc actgcgatgg gccgctcgat gaggatgggg tgggcgacaa
    12661 gggcgtccag ccattcgtcc tcgctgaggg tccggccctt gaaggtcgcc ttgaagacat
    12721 cctcaccctt gcgcaccagg tcttcggccc gcatgccgag cgccgccagc acggcagcca
    12781 gctcttcgcg ggtgggcggc gtcttgaggt actcgatgat cgtggtcggc acgcccttgt
    12841 cggccagcag ggcgcaggcg gagcggctct tggaacagcg ggcgttgtgg tagaggcgga
    12901 tcgtgtcggt catgatggat cgggtgttca gaacaggacg tccttggaaa cgccccggcg
    12961 cttgatgatg cggcgcagct ggccgagggc ttcgagctgg atctgacgca cccgttcgcg
    13021 ggtgaggtcc aggctgtccg ccagttcttc gagggtctgc acctcgcact cgtcgatgcc
    13081 gtagcggtgg cggatcacca tgcgctgctt ttcgttgagc tggccgatcc actcgcgcac
    13141 gaggctctcg acctccaggc cgtggatctg cgtgtcgggt gtttccgcgc cttcatccgc
    13201 cagggattcg ccgatggaga ggcttggatc gatatccagc ggcgcgtcga gggacgcggt
    13261 gtgctcgttg agggcgagga tggcgcgcac atcctccacg ggccggctga gctggcgggc
    13321 cacctcctcg acggtggtgt cgccatggcc ggacgcttcg agctgacgct gggtgcgcag
    13381 aacctggttg agctccttca ccacatgcac cgggaggcgg atggtgcggg actggttcat
    13441 gatggcccgc tcgatgttct gacggatcca ccaggtggca taggtggaaa agcggaagcc
    13501 ccgctccgga tcgaacttct cgagggcgtg gatgaggccc aggttgccct cctccaccag
    13561 gtcgaggagc gggatgccgc ggttgagata atgcttggcg atgttcacca cgaggcgcag
    13621 gttgcgctcg atcatcgtct ggcgggccgg gaagtcgccc tcacgaacgc ggcgcgagag
    13681 gctcagctcc tcttccgcag tgagcagcgg gttggcaccg atctcgttga gatagagctg
    13741 ggtgacgtcg ccaaggaatt ccgtatccac tggcgcgggg gtctcgagaa agacctcaac
    13801 ctccggcggc agatccggtt cgggggtctc cagctcttct tcaggcacgc ccggctcgta
    13861 catggatgtc ctctggggtc aaaggggcgc cggagggttt cccgccgccc gccccgcaat
    13921 gcccggcacg ccccgcttct tgacgagggg tagtgccgca ccaacacttc agatcatgtc
    13981 gcccacctgt tacggatgtg tcattgctca gcgggacggc aggtatttca caggatccac
    14041 cggcttgccc tgcttgcgga tctcgaagtg cagcttgggg cggtcgctgt ccgtgtcccc
    14101 cagttccgcg atcttctgac ccttgctcac cgactgccct tccttcacca gcaactgctg
    14161 gttgtgggca taagcgctca gataggcgtt atcgtgcttc acgatcagga gtttgccata
    14221 tcctcgcaaa cccgtaccgg catacaccac cttgccggcc gccgccgcat agacgggatc
    14281 gcccggcttg ccggagaggt cgatcccctt gctggaggta tcgttgaagc cggccatgac
    14341 cttgccaccg gccggccagg accattcgat gcggtcgtcg cccgctgccg cgggtttggc
    14401 ggcgtccttg cccttgtcgt cggccgtctc gggcttggct tccggcttcg tctcgggctt
    14461 cacgtccggc ttggcatcgg gcctggcgtc cgtccggggc tcggctttgg cgtcggcctt
    14521 gaccggcacg acaggtgcct ccggcttctg ggcctgagcc caggcctgct ccgaataggg
    14581 caccttgccg cccttgggct cgcggcgcag accgtctgct gacggcgcca caggtcgcac
    14641 gtcggcgccc gcgggcgcca ccggtttgac caccgcaccg gactccggcg gcttgacgcg
    14701 cagttcctgg cccacttcga tgagattggg gttttcgagg ccgctccagg cggcgatgtc
    14761 ccggtagttc tgaccatgat cgagggcgat gcggatcagg gtatcgccgg gcttgacgat
    14821 gtagtaaccg gggcgtgcca ccaccggtgc cggcgctgcg ggcgtttcgg cacggaccgg
    14881 cggcgtggca cccgccttgg cccggtccgc caccggagcg ggggacttgg tggcacaccc
    14941 ggcgagggcc agcgcagaca ggaacagact cagggacaga cgggacaaca tggttccttc
    15001 acggtgcttc attcggtacc gggcagcagc gggacgaatc gtaccgggct gagacgggtt
    15061 tccttggggc cgcggacggt gcgctcgatc agcacgagta cctgctcggc catgcctacg
    15121 ggcagaatca gacgcccacc ctcggccagt tgttccacca gggcgggcgg cacctttggc
    15181 gcggcggccg cgacgatgat ggtgtcgaaa ggggccgcat ccttgagacc gacactgcca
    15241 tccgcatgtt tcaggcgcac gttgggcagg cgcaggtggc gcagattggc gcgcgcgcgg
    15301 tcgagaagca ggcgcaggcg ctcgacggaa tacacgtcac tcgccaggga ggccatcacc
    15361 gcggcctggt agccgcagcc ggtgcctact tccagggtct tgcccaggct ttcgcggccg
    15421 gccagcagca gttcgagcat gcgcgccacg acaaagggct gggagatggt ctgctgcagc
    15481 ccgaggggca gggccgtgtc ctcgtaggcg cgggaggcca gggcctcctc gacaaaaagg
    15541 tgacggggca catgcagcag ggctgccagg acccgctcgt cgcgcacgcc ctgctcgcgc
    15601 aggcgctcga ccatgcgggc acgggcacgc tgggcgccgt gaacaccgac acgggccacc
    15661 tcgctcatga cgcgagccag tcctgaaccg gggcaatctg cccggcgtgg gtgagatcga
    15721 tctggagagg cgtgaccgac acgtagccgt gggccaccgc gtggaaatcg gtgccttcgc
    15781 cggcatcctg ggccgcaccg gcggcaccga cccaatacac cgtctcgcca cggggcgtga
    15841 ccgacttgat ggtgggctcg gccttgtggc gcttgccgag gcgggtcacc cggatgccct
    15901 tgatctggtc gtagggcaga tccggcacat tgacattgag cagcgtgggc tgacgcaccg
    15961 gctgacggat gaaacgcgcc acaagctcgc gagcgatgcg cgcagccgcg ctgaaatcgg
    16021 acgcctcctt cgacaccagg gacatggcaa tcgctggcac gccgaggaga taaccttcgg
    16081 tcgccgcggc aacggtgccc gagtagatcg tgtcatcccc catgttggcg ccatgattga
    16141 tgccggagac aacgatgtcg ggcagttgtt cgagcatgcc ggtgaccgcc aggtggacgc
    16201 agtcggtcgg cgtgccgttc acgaagtgaa aaccgttggc cgcgcggcgc agggacaggg
    16261 gccgatcaag ggtcagggaa ttgctcgccc cgctgcggtc ccgctccggc gccaccacac
    16321 tgatttcacc cacgtccccc agggcggaag ccagggcctg caggccgggc gcgaaatagc
    16381 cgtcgtcgtt gctgagaaga atgcgcatcg ggtatcgatc cggatcgggc ggaacaggtg
    16441 tcgcggaacg gaaaagggca ggaatcggaa aggccggcac agcccgcacg ctccgagcag
    16501 ggcgccggaa ctggcggcga gtttagcaga gtcggcttcg cagccgctag gcggaaaaga
    16561 cggactacac tgaaggagcg ccccgcggga caccgctgca gaatattttg cacgggacgg
    16621 aataaaccgg actgcccggt tgtttacgca tcacccattc cccaccggag acctcccatg
    16681 atgcaccgtt ccgtcacaca cgccctgatc ggcctgggcg ccctgctcgc cgccgcatgc
    16741 accaccctgc ccggactggc cactgcaccg gcccagagcc gcgacggcct cctcgccgac
    16801 gccaagggca tgaccctgta tgtgttcgac aaggacgccg ccggcagcgg caagagcgcc
    16861 tgcaacggcc cctgcgccaa caactggcct cccctcgtgg cggccgaggg cgacaaggcg
    16921 tcgggcgact ggtcggtgat cgtccgtgac gacggtcgcc gccagtgggc cttcaagggc
    16981 aagccggtct atacctgggt caaggaccag aagcccggcg acaccaccgg cgacggcttt
    17041 aacagcgtct ggcacgtggc ccgtccataa gcccccgccc cgatgcgccc atgagtttcc
    17101 gccaggccat cgtcgccgag attccccgcc tgcgccgcta tgcccgggcg ttgacggggg
    17161 acgccagccg cgccgacgac ctggtccagg acaccctcga acgggccctc ggcaagtggc
    17221 gcctgtggcg acccggcaac ctgcgggcgt ggctattcac cttgatgcac aacattttcg
    17281 tcaaccagct ccaggaaagc gtgctggtca tctccctgga tggcgacacc cacctggacg
    17341 taccggtccg cccgcgccag caggacaacc tggaactgcg cgatctggac cgggccctgg
    17401 cccgcctgtc gcccgagcag cgggaagtcc tgttgctggt gggcctggaa gagctcagct
    17461 acgaggacac cgcccgcgtc atcggcgtgc ccgtgggcac cgtgatgtcg cgtctggccc
    17521 gcgcccgcga caagctgcgc ctgctcctgg cgggcgacga gcgcgccctg cacctgaagg
    17581 tcgtcaaatg aacaccccgc cggatcgcga ccgcctgcac gccttcgccg atgaccggct
    17641 ccccgccgac gagcgggtgc gcgtggccgg ctggctcgcc gaccatcccg acagcgccgc
    17701 cgccgtccat gactggcgcc gccagaacgc tgccctgcac gcggcctacg acggcgtgct
    17761 ggagcaatcc gtgccgcccc atctcctcgc gccccccacg cccacgccgc gccccttgtt
    17821 gcgcgttgcc gccgtgctgg ggtggctggc cctgggcatc gtgatcggtc gcgtgctgcc
    17881 ccccgacggg cccgcagcga cagtggacga tccgctggcg gcgctgcccc accgcgcagc
    17941 cgtggcctac gcggtttatg tgccggaagt gcgtcacccg gtggaggtcg gcgccgacca
    18001 ggaatcgcat cttgtgtcgt ggttgtccaa gcgcctgggc acgccccttc gggcgccgca
    18061 tctcgtggcg gaaggcttcc gcctggtggg tggccgcctg atcccgtcgg agcagggccc
    18121 tggcgcactg ttcatgtacg aggacggcac gggcgcgcgc ctcacactgt atgtggtcac
    18181 cgtcgagcac gacggcggcg aaaccgcctt ccgcttcgct catgccgggg cgctgtccac
    18241 cttctactgg gtggaccggc acaccgccta cgccctgtcc ggggaactcc cgagggcgtc
    18301 cctgctgccc gtcgccagcg cggtctaccg ccagctcggc gaggatcgcc cgcccgcgca
    18361 aaaatagtcc ggatcttctg taagctgaac gcccggtcgc cggtcttgtc ccatgccacg
    18421 cgtccaagga ctttccccat gttgatccgc cgccccgccg acctgccgcc ctccgaaatc
    18481 accgaccccg ccgtatacgg ggcccgccgc cgcttcatgg gtcaggccgg cgccctcgcc
    18541 ctgggcagcc tgctgccggc tgccggacag gccgccctcg gccccctcgg ccccctcagc
    18601 cacagcgcct tgagtaccac cgaaacgccc acatccctgc gcgcggtgac cggctacaac
    18661 aacttctacg aactgggcac cagcaagacc gaccccgccg agaacacctt ccgcctcaag
    18721 cccgcgccct ggaccctgcg cgtcgaaggc ctggtgaaca agccccgcac ctttgccctc
    18781 gaagacctgt tccgcctcgc gccgctggaa gagcgcatct accgcatgcg ctgcgtggaa
    18841 ggctggtcga tggtcatccc gtgggttggc ctgcccctcg ccagcgtgct caagctggcc
    18901 gatccccagg gcagcgcgaa gtacgtggag ttcacctccc attacgaccg cgccaccatg
    18961 acccgcggcg tgctcgactg gccctactcg gaaggtctgc gcctggacga agcgatgcat
    19021 cccctgacaa tcctcgcggt gggcctctat ggagaggtgc tgccggtgca gaatggcgcg
    19081 ccggtgcgcc tggtcgtccc ctggaagtac ggcttcaagg gcgccaaatc cctggtcagc
    19141 ctgcggctgg tggacaagcc accgctcact gcctggatga aggccggccc cagcgaatac
    19201 ggcttctacg ccaacgtgaa tcccgacgtg gatcaccccc gctggagcca ggcgacggaa
    19261 cgccgcatcg gcgaattcct caaacgcaag accctgatgt tcaacggcta cgccgaccag
    19321 gtcgcctcgc tctacacagg catggacctg cggcgctact tctgacccac gatgaatccc
    19381 gacacccgcc agctcgccct cctcaagacc gccctgttcg ccctgtgcct cgtccctgcc
    19441 gtcctcctcg ggcgcgcctt cagcctggac ggactgggtg ccaatcccat cgaggccctc
    19501 acccgcgcca ccggcgaatg gaccctgcgc ctgctcctca tcaccctgtg cgtcaccccc
    19561 ctgcgcaagc tcaccggctg gcactggctc gtgcgcctgc ggcgcatgct cgggctgttc
    19621 acctttgcct acgcctgcac ccatctgctc acctacctgt ggctggacca gttcttcgac
    19681 tggcacgcca tcgccaagga catcctcaag cgccccttca tcaccgtggg catggccgcc
    19741 ttcgtgttga tggcgccgtt ggccgccacc tccagcaacg cggccatccg ccgcctgggc
    19801 ggtcgtcgct ggcaggccct gcaccgcagc atctacccca tcgcgatcct cgcctgcgtg
    19861 catttctggg tgctggtgaa gaaggacatc accgaaccgc tgctgtacgc cctcatcacc
    19921 gccgtcctgc tcggcctgag agcctggtgg cgcgagcagg aacgccgccg tcagctggcc
    19981 ggcgcctatg cacccccgcc ccaggcgcgg ggcaaggtga tccccatcgt cgccaagtaa
    20041 gcagcgcggc tttgccctag acttccaatt ttcataccgg aagcctgcca tgtcgctgga
    20101 tcactttgcc cacaaggctg acagtttcga cgccaatccc gtccgcgtag acaacgtgga
    20161 cgccatcgcc cgcgtcctgc gcgacaccgt ggccctggac cccgccatgc gcctgctgga
    20221 cttcggctcc ggcaccggcc tgctgctgga gcgtctggcg ccgcatgtgg cgcacatcac
    20281 cgccgtggac gtgtcgccgg ccatgaaccg ccagctcgac gccaagcgcg cgcacctgcc
    20341 ctgcacggtg gagatcctgg aactcgacct ggtcgcccac gatctggcgc agaccttcga
    20401 cggcatcgtc tcgtccatga ccttgcacca cgtggcggat gtggccgccc tgctgcgcaa
    20461 gttccacggc ctgctgcccg acggcggttt catcgccctg gccgatctgg acaccgagga
    20521 cggcagcttc cacagcgagg acaccggcgt cttccacgcc ggcttcgagc gggcctacat
    20581 cgccggcctg gcggcggcag ccggctttcg cgaggtgcag gtgcactccg ccagcgtggt
    20641 gcgcaaaccc cagggggatt acccggtctt cctgctcacg gccgtccgct gaatcgacgc
    20701 ctaccccctc agggcagggg cagaccgtcg ccgcccccgg cgccgtcctt caccgagcgg
    20761 atcagctcca ccagccggtt gggcttgccg cgcatcagca gcagcaactc ggcgcgcacc
    20821 acggattccg acaggccggc cgccaccaga ctgctcagca tctcggcgaa gcgggcgcgc
    20881 cgcttctgcc ctgccggcga ggtgtcgcgg cggtcgtgga agctttccag cagggttgag
    20941 ccacacacgc agttgcgaaa gacgtccacg atcacggcgc cgtcgtcgtc gtagctctgc
    21001 ttgagcccgg tgtgatcccg gcgcaccgga tgggtggcgg tgaggaattc ctcgtgggtg
    21061 cggtatttcc gcccgcagtt ggcgcaggtc ttgggaaagg ccgactcttc cagtgcgcgc
    21121 aggcgggcga agagggaatg ctggaaggac aggtcgctca cggggccctc gcggacgatt
    21181 ccggacggcc ggcgccgccc ggattcgatg ccgcgtgccc gtcaggcctt gggccggatc
    21241 gccgacttgg gacggaacgc cttgatcacg gcatcgtggg tttcgatgta cgcaccgccg
    21301 atcagatcga tgcagtaagg cacggcggcg aagatcccgt gtactgtcgg ccgtccctcc
    21361 ccatccttga gcccttcgag ggtttccgca atggccttgg gctggcccgg caggttgatg
    21421 atcagggtct tgccgcggat caccgccacc tggcgcgaca ggatcgccgt gggcacgaag
    21481 gccaggctga cctggcgcat ctgctcgccg aagcccggca tctccttgtg gccgaccgcc
    21541 agagtcgctt ccggcgtcac gtcccgcagg gccgggccgg tgccgccggt ggtgaggacc
    21601 aggtcgcagc ccaccccatc gcacagttcg atgagggtcg cctcgatctg cgcccgttcg
    21661 tcaggaatca ggcgggtttc cgctcgccac ggactggcca gggccgagcc aagccagtcc
    21721 tgcagcgccg ggatgccttt gtcctggtac acgccttcgg aagcgcggtc gctgatggac
    21781 accaggccga tgattgctgt ctcgctcatg cttcgtcctc tccggatggg gggtcgttgt
    21841 cgggtgcgcc ttcgagctca cgcagcacgc ggaacagttc gcggaaagcc cggggcggct
    21901 tgcccagctc gcgctccttg agggcattgc gccgcagcac gcggaggtgc tggagatcag
    21961 cgccgggatg cgcggtggcg atgtcgtgga gggtcttctc gtcatcgagc aggcgggtgc
    22021 gcaaggcttc caggcgatgc atgcgggcgt tttcctcggc cgagacgccc ttgaaggcgt
    22081 ccagcgcagc gcggatcggc gccgggtcca cgtgacgcat cagcttgccg atgtattgca
    22141 actgacggcg cttggcctcg tgggacggaa agcgctggta atcacgcacc gcgtcgcgca
    22201 ggtcttcgtc caggggcacc ttggccaggc gctccttgcc gagggcggcc agctcctggc
    22261 cgagttcctg cagggcctgg ctgtcgcgtt tgagctggga tttgctcggg ccggtgtctt
    22321 cgtcgtcgtc gtgatgggaa tggggatggg ccattgcggg cggttcgggg gactggaaac
    22381 ggttaagatt ataaccttga ccccgtacca ccccccgaga gccatggccg agaaaagatt
    22441 cagcttcacc caggacgaac tccgcaccct ggccgacgac atcctcaagt acgcccgcaa
    22501 gggcggcgcc tcggcctgcg aggtggacgt gtcggagggc ttcggccagt ccgtcacggt
    22561 ccgtcgcgac gaggtggaca ccatcgaata caaccgcgac aagggcatcg gcgtcaccgt
    22621 gtacgacggc cagcggcgcg gctacgccag cacctccgac ttctctgccg cctccctcaa
    22681 ggcgactgtc gcggcggccc tgtccatcgc ccgcttcacc gcccccgacc cagcggccgg
    22741 gctgcccgat gcggcgctgc tggcgcggga catcccggac ttcgacctgt accacccgtg
    22801 ggcgctgtcc tccgaagacg ccatcgagct ggcccgccgc tgcgagcggg cggccttcgc
    22861 ggtcagcccg gaaatccgca attccgacgg cgccagcgtg tccatgcagg aatcccactt
    22921 catctcggcc aacagcctgg gcttcatggg cggctactcc agttcgcgcc acttcctgtc
    22981 ctgctcggtg atcgccggcg agggcgacga catgcagcgg gacgactggt attcctccaa
    23041 gcgcgacccg gccgagctgg ccgacgcgga agaggtgggc accttcgccg cacgccgcgc
    23101 cctggcccgc ctcggcgcgc gccagattcc cacctgcgag gtgccggtgc tcttcgaagc
    23161 ccccctcgcg gccggcctga tcggtgcttt cgtgcacgcg gtaagcggcg gcgccctcta
    23221 ccgcaagtcc agcttcctgc tggacagcct gggcaagccg gtcttccccg ggcacatcca
    23281 gatctccgaa cgcccgcacc tgcccaaggc attcgccagc agcgccttcg acgacgacgg
    23341 cgtggccacg cgggaccggg aagtcatcgt ggacggcgtg ctccagggct acttcctgag
    23401 cacctactcg gcacgcaagc tgggtctcga gaccaccggc aatgcgggcg gcagccacaa
    23461 cctgctggtc aagcccggcg cccacgacct ggacgggctg gtgcgccaga tggaccgggg
    23521 cctgctcgtc accgagctgc tgggccacgg cgtgaattac gtgaccggcg actactcccg
    23581 cggcgccgcc ggcttctggg tggagaacgg gcagatccag ttcccggtgc acgagatcac
    23641 catcgccggc aatctcaagg cgatgctggc cggcatccgc gagatcggct cggacgtgct
    23701 ggtgcgcggt tccaagcact gcggctccat cctcgtggac cgcatgaccg tcgcgggcgc
    23761 ctgagcccca cccgctgcgg gccttgacgg agacaccgga gatggaacgg cgtcgattcc
    23821 tcacgcaggc cggtgccggc atggcggccg gcgccctcgc ggctccggcg gcggcggcca
    23881 tggccgacct cccgatcctc aaatggcgcc tcgcctccag ctttcccaag agcctggatt
    23941 cgattcacag ctcgacggaa tccctggccc ggcaggtggc gctgctcacc ggcggacgct
    24001 tccagatcca ggtcttcgcc gccggcgaac tcgtccccag cgcggcggtg ctggatgcgg
    24061 tcaaggacgg taccgtcgag atcggccaca gtgcgtccta ttactatgtg ggcaaggatc
    24121 ccaccttcgc cctcgacacg ggcatccctt tcggcctcaa cagccgccag cagatggcgt
    24181 ggatgcatga cggtggcggg ctcgaactgc tgcgcgactt cttccgcgag tacgccatcc
    24241 acaacatccc ctgcggcgcc accggcaccc agatgggcgg ctggttccgc aaggagatcc
    24301 gcagtgttga agacctcaag ggcctcaagt tccgcgtcgg cggctttgcc ggtcaggtcc
    24361 tcgcccgcct cggcgtggtg ccccagcaga tccccgccgg cgacatctac gcagccctcg
    24421 aaaaaggtgc catcgacgcc gccgaatggg tcggccccta cgacgacctg aagctcggtt
    24481 tccagcgggt cgccaagtac tactactacc cgggcttctg ggagggcggt ccccagctca
    24541 ccgtttatgt gaatctcaaa cactggaacg ccctgccgcc ggaataccag gccgcactgg
    24601 agaacgcctg cgccctcgcc catttggaga tgcaggcccg ctacgacgcc cgcagcccgg
    24661 ccgccctgcg ccagctcatc gcctcgggca cccagctgcg ccccttctcc cacgagatca
    24721 tgagcgctgc ctaccaggcc gcccacgcac tctacgacga aaccgccgcg aaaaacccgc
    24781 gcttccgcaa gatctacgag ccctggaaga aattccgcga cgaccagctg cagtggttcg
    24841 ccgtggccga gagccacttc gacgccttca tgcaggcgac gcgcgccgcc gcccgcagcc
    24901 gcaaataagc ccggcgggcc ggattggggt tttcccatac tgcgggaagg ccgccggctc
    24961 atgaataatc ggcatccaga ttccaacacc acacgaggag acaaagtgga acgacgttca
    25021 ttcctgaaga aggccagtgc cggcgtcgcc gccggtgcgg tggccgcgcc ggccctggcg
    25081 gccgaactgc ccaccatcaa atggcgtctg gcctccagtt tccccaagag cctggacacc
    25141 atcttcggcg gcgccgatgt ggtggccaag cgggtggcgg ccgccaccgg cggcaagttc
    25201 cagatccagg tgttcgccgc cggcgagatc gtccccggtc ccggcgtgat ggacgccgtg
    25261 aaggacggca ccgtcgagtg cggtcacacc tgctcctact atttcgtcgg caaggacccc
    25321 accttcgccc tcgacacggc catccccttc ggcctcaaca gccgccagca gaccgcctgg
    25381 atgatggatg gcggcgggct ggaactgttc cgtgagttct tcaaggacta caacatctac
    25441 aacatcccct gcggcaacac cggcgcccag atgggcggct ggttccgcaa ggagatcaag
    25501 agcgtcgagg atctcaaggg cctcaagttc cgctgcggcg gcttcgccgg ccaggtcctc
    25561 gcccgacttg gcgtggtgcc ccagcagatt cccggcggcg acatctaccc ggccctcgaa
    25621 aaaggcacca tcgacgccgc cgaatggatc ggcccctacg acgaccagaa gctgggcttc
    25681 aacaaggtcg ccaagtacta ctactacccg ggcttctggg aaggcggccc gcagatcagc
    25741 ctgtacgtga acatgaagca gtgggcggcc ctgcccaagg aataccagac catcctggaa
    25801 gacgcctgcc tctatgccca cgccgagatg caggcccgct acgacgccaa gaacccgggc
    25861 gcgctcaagc agctggtcgg ttccggcact cagctgcgcc ccttcccgaa cgacgtcctg
    25921 gccgccgcct acaaggccgc caacgacctc tacgacgaaa ctgccgccag caatccgaag
    25981 ttcaagaaga tctacgaggc ctggaagaaa ttccgcgacg accagctgca atggttcgcc
    26041 gtggccgaga accgcttcga caacttcatg caggctgccc gcaacgcctc ggccaaggcc
    26101 aagaagtaag cgcccgccac cccctgggcg ggtttgatcc gggccgtgtc cgcacggccc
    26161 gcttttttga ggaagaccgc ccatgattgc cgccttcgcc ctcctcctgg gcttccagct
    26221 tgctggggaa accctgcgtg ttgccctgca tctgccggtg cctggcccgg tgatgggcat
    26281 ggcgttgctg ctcgtctatc tgatcttccg cggcggtccg gacgatggcc tgcggaccac
    26341 ctccggcggc ctgctgcagc acttgtccct gctctttgtg cccgccggca ccggggtgat
    26401 gctccacgcc ggacgcctcg ccgacgaagc cggcccgatc ctcgtggccc tcgtcgccag
    26461 caccgtgctg ggcctcgccg ccaccggcct gaccctgcac gctctcaccc gccgcagtcg
    26521 ccaggaagac gcgcaatgag cagcgggatg accgaaatct gggtgtacct ctccgcccaa
    26581 cccctgttgt ggctctccct cacgctggcc gcctacctcg tggccctgac cctgcaccgc
    26641 aaggcgggag ctcatccggc ggtcaacccg gtgctgctgt cggtgctgat cctggtcggc
    26701 ctgctcgtcg ccaccggcac gccctaccag cactacttcg acggcgccca gttcgtgcat
    26761 ttcctcctcg gacccaccac cgtcgccctc gccgtaccgc tgcacgccca gctcaagcgc
    26821 ctcgtcgcga tgggcggccc gctcctcatt gccctcgccg tgggctcgct gaccgccggc
    26881 ctgtcggcct acgccatcgg cgccctgctg ggcgccagca caccgacggt gatctccctg
    26941 gcgccaaaat ccgtcaccac gcccatcgcc atgggcatcg ccgagcggct cggcgggctg
    27001 gcgtccctga ccgcagtgtt cgtgatcctc accggtatct tcggtgccat cttcgccacc
    27061 ggcctgctgc accgcctggg cgtcaaggac gaggccagca cgggcttcgc cctcggcctg
    27121 gcgtcccacg gtatcggcac tgcgcgggcc ttccagctgt ctgagcagac cggcgccttt
    27181 gccgccctgg cgatgggcct caacggcctg ttcaccgccc tcacgctgcc ctggctgctg
    27241 cccctcctcg cccccctctt gagccgctga accatggacc tgctcatcga cgtcatcctc
    27301 aaggcgggcc gctccgccgt cgaactgtcc ttcttcgtgc tgctacccgt gatggtggtg
    27361 atgctgtccc tgatgcgcct actcgaagcc cgcggcgtgc tggactggct ggtggaaaag
    27421 ctcgccccgg ccctgcgtcc gctgggtcta accgggctcg gcgtgttcgc ggcgctgcag
    27481 atcaatttcg tgagctttgc tgcccccgtc gccaccctgg cgatgatgga ccagcgtggc
    27541 gcgtccagcc gccatctggc ggccaccctg gccatggtga tggccatggc ccaggccaat
    27601 gtgtccatgc cgatggccgc catgggcctg cacttcggca tcgtgctggg ctggtcgctg
    27661 gtaggcgggc tggtcgcagc tgccgccacc tattacggtt tcggccggca tctgagcgcg
    27721 gtcgaacaca ccctcgacga aacgctcaat cacccggtgg cggaatccgc caagggcgtg
    27781 ctggacgtca tcaaccgggc cggcgccgag gccttcaaga tcgcggtggg cgcgatcccc
    27841 atgctggtcc tgtccctgac cgttgtactc gccctgcgcc gcctgggcgc cctcgacgtg
    27901 ctcaccggcc tgctcgcgcc gatcctgctt gcggttggcg ccgatccggc cctgattctg
    27961 cccacgctga ccaaatacct ggccggcggc acggccatga tgggcgtcat ggacgagatg
    28021 ctgcgggccg gcacggccac cgccgccacc ctcaacggcg ccagcgccgg cctgctgatc
    28081 catcctctcg acattcccgg cgtggccgtg ctgatatctg ccggccggcg cgtggccgat
    28141 gtgtggaagc ccgccgccct cggggccgga gtgggcattc tggcacgggt cgccggacac
    28201 cttgtggtcg gctgagaccc cggcagccct ccccctgcgc gcagaccacc tgagtggcgc
    28261 ggaacatgca tcgcacaccg ggcttgccac gccatttcat taggcgaaat ggcgccttct
    28321 gcgctaccat gcgcgctcct tcccgatccc ggccgaaacc gttgtccacc gcgcctgaac
    28381 ccgccgccaa gaatcccatc caggtcatcg agcgcatgat gaagctgctc gacgtgctgg
    28441 cggaatactc cgatccggtg ccccttaagc agctcgccct ggagaccggt ctgcacccct
    28501 cgaccgcgca ccgcatcctg ggcgccatga gtggcagcgg tttcgtcgaa cgcagcgagg
    28561 ccggcgccta tcgcctgggc atccgcctgc tggaactggg cagcctggtc aaatcgcgca
    28621 tctccctgcg ggaaaccgcc atgccggcca tgctccggct gcacgccgaa accggcgaga
    28681 gcgttaacct gggcatccgc gccggtgacg agatcgtcta tgtggaacgg acctccagcg
    28741 gccgctcgtc ggtgcgcgtg gtccacatcg tcggcgcccg tgcccccctt cataccaccg
    28801 ccaccggcaa gctctttctt gtggaggacg gcgcccacca ggtcaaggaa tacgcgcgcc
    28861 gcaccggcct gcccggcacc acgcccacct ccatcaccac cctcgcggcc cttgagaagg
    28921 aactggacaa ggtgcgccgc cacggcgtgg cctacgatct ggacgaggtg gaaaacggcg
    28981 tgcgctgcat cgccgccggc atccgcgacg atagcggcga gttggtggcc ggcctgtccc
    29041 tgtccacccc ggccgagcgc ttcaatcctg actgggcgcc tctcatcaag gccacggccg
    29101 acgagatctc ccgcgccctc ggctacgtcc cgccggccgg gcgctgatcc acggcaccca
    29161 tgaaaggggc tcccgcggga gccctttttc ttgatcggcg ggataccgaa tcaggtggtc
    29221 ggaccgccgc gcaccacctc cgccagacgc tgctgggctg ccgcgctctc gagccagcgc
    29281 ttgacccgct gggcatccgc cacacgggtg aagcggccga aggagtcgag cagcacgatg
    29341 atcatcggct tattgagcag ccaggcctgc atcaccaggc atttgcccga ctcgttgatg
    29401 aagccggtct tggagacgcc gatctgccag tccgggctct tcaccagtgc gttggtgtta
    29461 ccgaactgac gtggccggcc gttgagatac acggtgtagt tgccagtggt ggagaactcg
    29521 cggatcagcg gatagcggga cgccgcggac accatcttga ccagatcgcg ggcgctggac
    29581 acgttggcgg agttcagccc ggtgctgtcg gaaaagcggg tgtccttgag gcccagggac
    29641 accgccttgc ggttcatcgc cgccacgaag gccggcaaac cgcccggata atgccgggcc
    29701 agcgcagatg cggcacggtt ttcagaggac atcagggcca ggcgcagcat gtcctcacgg
    29761 gtcaggtcca cgcccaccgg cagatgggaa ctgctgcccc gcagggtatc cacgtcgtcg
    29821 ttggcgacgg tgaggacttc gcccagattg aggcgggcat ccagcaccac catggcggtc
    29881 atcagtttgg tgatggaggc aatcggcagc acagcgtcgg aattcttttc cagcagcacc
    29941 gtgccggtgg cctggtccgc caccatgaag gccgcggacg ccaggaccgg attgccttca
    30001 tggtccatct ccggcgccac cgcgagggca gcggtacgct gcacctgggg cgatgcgaaa
    30061 gcggcgcggg cggcggcccg gttacgggcc ttggcgttct tggccggcgc gggcgccgcc
    30121 ttggccacaa aagatttctt ggccggacgg tgcgtggcgg ccaaggcgtc cgccggaccc
    30181 gtggcctgga gcgcgacagc accagccagc gccaggagcg atttgcgaat gttcattgtg
    30241 ttacgtcgat tctgattccg ggtgagggga aggcgaggcc tccccgaaag atccggccca
    30301 cctgccggcg tacgtccccg cacgcctgag acggaccgcc gccgagttcc aattctaacg
    30361 gcagacttct atttaaaata aaggcttatt cagaaaattt aaagtttttt ctgaatctgc
    30421 actcgtagct agcaattcta acgcatgaag ggctcaatcc gacgaccggc cttgcacgga
    30481 atcaagtccg aggaagcgcg cgatctcgcc gcgccgggcc atggcgtcgc gatagcccag
    30541 ttccaccagg gcgcgggtat aggaccgctc gaagagcaga taggaaagca gcatcgaccc
    30601 gttcctgcgg gtggccccga caccgcgcag gaccaagcgc agcgccggcg gcagggcgtt
    30661 gcggtagcgg gcagcgatgt actcgagccg ctgggacggc gcgatcacca gcgtctcgat
    30721 gggtctcaac gggatgccgg cagcctctcg ggcccccggc ggtagtgctg ccagggttgc
    30781 gttgatgcgc tccaggcgct ccagatcggt ggtcagccca tccaggaaga tgctcgccat
    30841 cgcatgcccc gccacctggg ccggcgacgg gtagtcgtag gtccgctgcc gcgcctcctc
    30901 gccacgccgc ccggcgccga tcaccagaat gcgccgtgcg cccagatgga tggccggact
    30961 gacgggtgcc tgctggcgca tggagccatc accgaagtat tcccggtgga gccggaccgc
    31021 cgggaacacg aacggaatcg cgctggaggc cagcagatga tcgaccgcca gactggcgcg
    31081 cagcccgacc cgctgggcgc gtcgccaggg ctccagcgcc gggacgccct gaaagaacga
    31141 caggctctcg cccgaggtat agccggacgc cgtgacggac acggcacgca gatggccgtc
    31201 cgcgatggcg cggtcgatgg cgccgaaatc cagcatcgcc gtcagcagcg cgcgaagcgg
    31261 ctcgttgtcc agcagggagc ggggcacctg atggaacaac cagcccagga agatcgccga
    31321 cccccagcgc gtgaccgagc gcaccaccgc cgcagtgtcg gcgcgataca cctgatccac
    31381 gtgaaagttg cgccagatga acgccagctt gcgcacagcc gcatcgaagt cggccgaata
    31441 ggccgcgagg gccaccgcat tgacggcacc cgctgacgtt ccgcacagga tcgggaatgg
    31501 attgccggag cgtttgcccc agacatcgcg gatggccacc aggacgccca cctgataggc
    31561 cgcccgagcg ccaccgcccg tgagtaccag cgccgtccgc ccgtccgtca tgcccctcct
    31621 ccgtgcgccg acattccgtc acgcccaccc gcgaccgcgg gcgtcatgcc cctttcaagc
    31681 agtgataacg gccgcctgga gcgtcggcgc agggttgacc cggccgcggg cgccatcacg
    31741 cggccccggc ggccgtgccg gcgactcagc ggccggcttc gacgacggtc aatgccgtca
    31801 tgttgacgat gcgccgcacc gtggcggtgg gggtgaggat gtgcaccggc ttggccgaac
    31861 ccagcaggat cggccccacc gtcatgccct cgccggcagc cgtcttgagc agattgaagg
    31921 cgatgttggc cgcgtcgagc gtggggaaga tcagcaggtt ggcgtcctcc cgcatgcggg
    31981 cattggggaa gatctgctgg cggatgctcg catccagcgc ggcgtcgccg tgcatctcgc
    32041 cttccacctc cagatcggcg tgttccgcgt gaagcattac cagggccttg cgcatcttct
    32101 cggccgtcgg cgaattgtcc gtaccgaaac tggaatggga cagcagcgca atgcggggct
    32161 ggatgccgaa acgacggacc tcgtcggcgg cgaggcgggt catctccacc acctgttccg
    32221 gtgtcgggtc gtaattgacg taggtgtcgc acagaaacac cgtgcgtccc ggcaaagaca
    32281 gcagattcat cgtgtagaag ttacgcacgc cggtctggcg gccgagcacc gtctcgatga
    32341 agcgcaggtg caggccgtgc atgccgtagg tgccgcagat caatccatct gcgtagccga
    32401 acttgaggag cagcgcgccc accagggtgg gtcggcgacg cacctcgcgc ttggcgtaat
    32461 ccacggacac acccttgcgc tccatcaagg catggtaatg ggtccacagc tggtcgtagc
    32521 gcggatccgt ctcggggttg accagttcga aatccagttc ggggcgcagg cgcaggccgt
    32581 aacgatcgat gttttccagc gtcacgtcgg gccgaccgat caggaccgga cggcacaggc
    32641 cctcgtccac gaccgtctgc accgcgcgca gcacccgctc cgactcgccc tcggcaaaga
    32701 tgatgcgctt gggcgagcgc ttggcggcct gaaagaccgg cttcatgacg aggccggatt
    32761 gccatacgaa attgttgagc tgctgacagt aggcgtcgaa gtcggcgatg ggccgcgtcg
    32821 ccacgccaga cgccatcgcg gcgcgggcca cctccggcgc gatcttgacg atcaggcgcg
    32881 gatcgaaggg cttggggatg atgtactcgg gcccgaagcc ggacacttcc tggccgtagg
    32941 cagcggcgac gatgtccgag gcctcggcac gggccaactc ggcgatggcc ttgaccgcgg
    33001 ccagcttcat ttcatccgtg atcgtggtcg cgccgacgtc cagtgccccg cggaagatga
    33061 aggggaagca caggacgtta ttgacctggt tcgggtaatc cgaacggccg gtggccatca
    33121 gcgcgtcgtc acgcaccttg cgcacctctt cgggcaggat ctcgggcgta gggttggcca
    33181 gggccatgat cagcggccgc gaggccatct tcgccaccat ggcgggcttg agcaccccgc
    33241 cggccgacag accgaggaag acgtcggcgc cctcgatgac ctcgcccagg gtacgcgccg
    33301 aggtcgtctt ggcgtagcgg gccttgatgg ggtccatcag cacggtacgg ccttcataga
    33361 ccacgccctc gatgtccgtc acccagatgt tctccaccgg cacgcccagc atcaccagca
    33421 gatccaggca ggccagcgcc gcggcaccgg cgccggaggt caccagcttg acctgcttca
    33481 gatccttgcc cagcagatgc agaccgttca ggaaggccgc gcccaccacg atggccgtgc
    33541 cgtgctggtc gtcgtggaag accgggatct tcatccgctc gcgcagcttc tgctcgatgt
    33601 agaagcactc gggcgccttg atgtcctcca ggttgatgcc gccgaaggtg ggctccagtg
    33661 cggcgatcat gtcgatcagc ttgtccgcgt cgggctcgtc gatctccagg tcgaagacgt
    33721 cgatgccggc gaacttcttg aacagcacgc ccttgccttc catcaccggc ttggccgcca
    33781 gcggaccgat gttgcccagg cccagcaccg cggtgccgtt ggtcaccacg ccgatcaggt
    33841 tgctgcgcgc cgtcaggctg gccgcctcgg ccggatcttc gacgatggca tcacaggccg
    33901 ccgccacgcc aggcgaatag gccagcgaca agtcctgctg gttggtgagc gccttggtgg
    33961 gcgcgatgga gatcttgccg ggcttgggaa ggcggtggta ttccagcgcg gcaaggcgga
    34021 tgcgttcgtc catggggtgt ctccctcttc ggtgtgggtg tgctgcgatg atccgactaa
    34081 acggacattt tggccatccg tgacttccac agcatttcat gcgaaatgcg tgatttttgt
    34141 cctcgaccga accgtggcat aatattaggc ttcctccttt ggggaatcgc ccgcacactc
    34201 gccaacaata cgcgccgagc cttgatggcc caaagacgcg atcgccgccc ggcggccgcc
    34261 ctctcaccgc cggacagagc aactcacgaa tctgtaacgt cctggagaat cgtctgatgt
    34321 ctcaaagcat tgccgaagca gccattgccg ccgcgcctgc ctgtgtcaag aacgaaaagc
    34381 tcaagcagtg ggtcggtgaa atcgccgccc tcaccgagcc cgatcgcgtc gtctggtgcg
    34441 acggctcgca agaagaatac gaccgcctct gcgccgagat ggtcgacgcc gggatgctga
    34501 tcaagctgaa cccggaaaag cgcaagaatt cctacctcgc ctggtccgac ccctcggacg
    34561 tggcccgtgt cgaagaccgc accttcatct gctcccagaa caagggcgac gccggcccca
    34621 ccaacaactg ggaagacccg gccaagatgc gcaagaccct gaacgaactg ttcaagggct
    34681 gcatgcacgg ccgcaccatg tacgtcatcc cgttctccat gggccccctc ggctctccca
    34741 tcgcccacat tggcgtggag atctccgaca gcccctacgt ggtgaccaac atgcgcacca
    34801 tgacgcgcat gggcaaggcc gtctatgacg tgctgggcgc cgatggcgag ttcgtcccct
    34861 gcgtgcatac cgtgggcgct cccctcggcc agggcgagaa ggattcccgc tggccctgca
    34921 acccggacac caagtacatc gtccactacc ccgaaacccg cgagatctgg tcctacggtt
    34981 ccggctacgg cggcaacgcg ctgctgggca agaagtgctt tgccctgcgc atcgcctcca
    35041 acatggcccg cgacgaaggc tggctggccg aacacatgct gatcctcggc gtggaaagcc
    35101 cgcaaggcga aaagacctac gtcgccgccg ccttcccctc cgcctgcggc aagaccaact
    35161 tcgccatgct gatcccgccg cgctccttcg acggctggaa gatctacacc gtcggcgacg
    35221 acatcgcctg gatcaagccc cgcaccgacg cggacggcgt gacccgcttc tacgccatca
    35281 accctgaagc cggctacttc ggcgtcgccc ccggcacgtc cgaaaagacc aacttcaacg
    35341 ccatggcgac gctgaaggaa aacatcatct tcaccaacgt cgccctgacc gacgacggcg
    35401 acgtgtggtg ggaaggcatg tccaaggaag ccccggccca cctgatcgac tggcaaggca
    35461 aggactggac ccccgagctg gccaaggaaa ccggccgcaa ggcagcgcac ccgaacgccc
    35521 gcttcaccgc cccggccgcc cagtgcccgt ccatcgaccc gaactgggaa aaccccgaag
    35581 gtgtgcccat ctccgccttc atcttcggcg gccgccgtgc caccaccgtg ccgctggtgt
    35641 accaggcctt caactggaac ttcggcgtgt acatggccgc caccctcggc tccgaaacca
    35701 ccgccgccgc cttcggcgcc cagggcgtgg tccgtcgcga tcccttcgcc atgctgccct
    35761 tctgcggcta ccacatgggc gactacttca accactggct gaagatgggt cacgcggtgg
    35821 accagacccc gggcatcttc tgcgtcaact ggttccgcat gaatgagaag ggcgaattca
    35881 tgtggcccgg cttcggcgag aacatgcgcg tcctcaagtg gatcgtggac cgtgtccgcg
    35941 gtcgcgtcgc cgcccaggaa aacgccctcg gctggatgcc ccagtacgag gacatcgact
    36001 ggaccggttc cgacgtgacc aaggccgagt tcgaggccct caccgccatc gacgccgacg
    36061 cctggaagac cgagctggcc tcccacaagg aatggttcga caagctgcag gaccgcctgc
    36121 ccaagcagtt cgtgctcaag cgcgaactgt tcgagctggc cctgtcggtc taagcttgtc
    36181 cggccgtcag gccgttgccg tacccgaaaa agcgccgagc aatcggcgct tttttgcttt
    36241 gcgaaggaaa tcccgatgac ccgcttcgcc cgtcgcatgg ccgacatcca gcccttccac
    36301 gtgatggaga tcctcaagcg cgcccacgaa ctggccgccc aggggcgcga catcatccac
    36361 ctggaggtcg gcgaaccgga ttttcccagc ccgaaaccgg tcgtcgaggc cgcccgctgc
    36421 ttcctcaacg gcggtcatgt ccattacacg ccggcgctgg ggctgagcgc cctccgcgaa
    36481 gccatcgccg gcttctaccg ggatcgcttc ggggctgacg tggcgcccga gcgcatcatc
    36541 gtcacccccg gcgcctccgg cgccctgatg ctggccctcg ccgtgctcac cgaccccggc
    36601 gacgcctggc tgctgcccga tccgggctat ccgtccaacc gccacctggt gcgcagcttc
    36661 gaaggggtac cggtcgccct gccggtggat gcgacgaccc gcttccagcc tacgcccacc
    36721 caggtggctg aagcctggac gacgcagacc cgcggcctga tggtcgccac gccggccaat
    36781 cccacgggca ccctgctcac gccgggcgag atcgtcgccc tccacgcggc cgtaacggcc
    36841 cgccatggcg tattgatcgt ggacgagatc taccagggcc tctgctatgg cgtcgacgcc
    36901 accaccgccc tcacccgcct tgacgacgtc ttcgtggtga acagtttctc caagtatttc
    36961 ggtatgaccg gttggcgcct gggctggctg gtcgcgccgc cggactacgt gcgcccgctg
    37021 gagaagctgg cccagcactt cttcatcgcg ccctccacac cggcccagca cgcggctctg
    37081 gcggccttcc ggcccgacac cctggcaatt ctggaagccc gccgcgaggc cttcgcgcag
    37141 cgtcgcaaca cccttctgcc agccctgcag acactcggat tccgcttcgc caccgagccc
    37201 cagggcgcct tctatctgta cgccgacatc acccacctgg cggacgacag tcagcagctt
    37261 gcccggcgcc tgatcgagga ggccggcgtc gccaccacgc cgggtatcga cttcggcgac
    37321 cacgggccgg agcgccacct gcgcatcgcc tatacgacgg acgagaaccg tctgctggaa
    37381 gccgccgcgc gcatccgccg cgtactcggc ctttaaagac ggaccatcgc cgtcaacgac
    37441 gaagggcccg cacaggcggg cccttccaga ggtcgaccta aaaagtcggc cctgaaatat
    37501 tcaactttcc acgccaagcc gcccgccaag cggccagg
//
LOCUS       DAOQMM010000024        36365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_292_length_36365_cov_49.741889, whole genome shotgun sequence.
ACCESSION   DAOQMM010000024 DAOQMM010000000
VERSION     DAOQMM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 36365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36365
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_292_length_36365_cov_49.741889"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /gene="ruvB"
                     /locus_tag="PLH85_06165"
     CDS             complement(<1..620)
                     /gene="ruvB"
                     /locus_tag="PLH85_06165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNB64179.1"
                     /translation="MIETDKLKGGAPDPAARLIGARSNGAQEDALERALRPKRLADYV
                     GQQKIRDQLEIFIQAARRRSEALDHVLLFGPPGLGKTTLAHIVAAEMGVNLRQTSGPV
                     LERAGDLAAILTNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSV
                     KLDLPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYTAEELAWIVSR"
     gene            complement(617..1681)
                     /locus_tag="PLH85_06170"
     CDS             complement(617..1681)
                     /locus_tag="PLH85_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNB64180.1"
                     /translation="MAPRRTAQIAIIALLLLGCAFVLAPFFAAILFAGTVCITTWPMY
                     AALHRRLGRRDGLAAISMTVLLTAVLLLPMIGLTASLTDAVAEGVDQAREAIEQSDGK
                     PPAWLKDIPVVGPDVDVYVRRLTRNHEEARKLALQALEPARKFALGAARVVGQGLLQI
                     SLVLFIVFFLYRDGERLARMLDTGALRLGGELGERLLELAGNTVKAVMIGIVGTAAAQ
                     ALVALAGFLVAGVPGALLLAAGIFFLSMVPVGPPLIWGGAAFWLYQQDQSGWAIFMVL
                     WGTLAVSSVDNFLKPMLISRTASLPILLIALGAFGGVLAFGFIGLFLGPTFLALGQAL
                     VREWTAIEPQIAAPAAEDEA"
     gene            complement(1777..2268)
                     /locus_tag="PLH85_06175"
     CDS             complement(1777..2268)
                     /locus_tag="PLH85_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNB64181.1"
                     /translation="MSRKLRQGFLRNVQIGAGSLRLLMNLWPPFIGAGIHVERIAPDF
                     TEAVVRLRLGLLNRNAMGTHFGGSLYAMTDPFFALLVMYQLGRGYVVWDKASRIDYKL
                     PGRGTVYATFRVPPAQVDAIRHATAGGDKVEPVFEVDVLNRRGEVVATVEKTVYVRRR
                     PAD"
     gene            complement(2265..2861)
                     /gene="ruvA"
                     /locus_tag="PLH85_06180"
     CDS             complement(2265..2861)
                     /gene="ruvA"
                     /locus_tag="PLH85_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNB64182.1"
                     /translation="MIGRLTGRLAEKAPPQILLDVNGVGYELEVPMSTFYGLPATGQT
                     VTLATHLAVREDGHFLYGFASEDERAAFRQLLKVAGVGARTALAVLSGLSVSDLAQAI
                     ALQESGRLIKIPGIGKKTAERLLLELRDKLPKALPGLAAAGGLATAAPGDGRSDIVHA
                     LLALGYNEKEALAAVKTLPEGVGVSDGIRQALKGLSKA"
     gene            complement(2874..3485)
                     /locus_tag="PLH85_06185"
     CDS             complement(2874..3485)
                     /locus_tag="PLH85_06185"
                     /inference="COORDINATES: protein motif:HMM:NF025850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNB64183.1"
                     /translation="MKLVGMLDSPFVRRAFISGRMLGIPFEHQSLSVLRQVEEFRALN
                     PLVKAPTLVFDDGEVMVDSSQIITLFESLAAPGSSLLPAAPADRRRCLQLTGFGLAAA
                     EKALHRVLETRIRPKEARFPEWLARTERQLRDTWAMIDARVGDADWLCGDAPTQADIT
                     VAVAWRFTHYSAPGFLADGDYPHVAALAADAETLPAFVAADFT"
     gene            complement(3498..3812)
                     /locus_tag="PLH85_06190"
     CDS             complement(3498..3812)
                     /locus_tag="PLH85_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64184.1"
                     /translation="MRRVRAALLAFGLPACAAAQAPGSTAFCLFPLPAEAGAQRFINL
                     GIVQYVEVRADEVRLAYGGGNLGSGHDARIPIRSREEGEAVLDRLRRTASACRFPSPT
                     PP"
     gene            complement(3809..4492)
                     /locus_tag="PLH85_06195"
     CDS             complement(3809..4492)
                     /locus_tag="PLH85_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNB64185.1"
                     /translation="MSPSLPFLPGDIDGVIFDMDGLLLDSERVALTIIEATAAELGLP
                     WRHEVGLAMVGLNSRDSPAVVRSHLGADFPVEALMDACGVHYERAIAAGRIPLKPGVE
                     ALFDVLDTLGLGRVVATSTRRVRAEPKLAAVGLLPRLHGMVCGDEVSRGKPAPDIFLA
                     AAARLRLPPARCLVLEDSNAGVRGALAAGARVVMVPDLLQPADDVRAAAVPVAASLAD
                     LADFFGRAP"
     gene            complement(4489..5463)
                     /locus_tag="PLH85_06200"
     CDS             complement(4489..5463)
                     /locus_tag="PLH85_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNB64186.1"
                     /translation="MASAPPASPAEPRVVLRTRPVLAAFLATRPAFLGVTLCACLVGL
                     ATAHGSGVIVDSTRAALTIAFALFAHAGINVLNDYYDARSGADAANTERVFPFTGGSR
                     FIQNGVLSEAETARLGWGLLLPVVPAGLWLAAQSGIGLVALGVAGLFLGWAYTAPPLA
                     LVSRGLGEIAVAAGWLIVVAGSDFVQRGAWSFLPVAAGLSYALLVAAILFFNEFPDHR
                     GDAAAGKRTLVVRFGPDGAKWACVGLVVGAYAWLMLMVGRELLPHAAAAAAFSLILSF
                     RALRHLLAHAAEPAALAPALKLTIAAAHLHGLILAATLAFARWPGGGA"
     gene            5882..7051
                     /locus_tag="PLH85_06205"
     CDS             5882..7051
                     /locus_tag="PLH85_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64187.1"
                     /translation="MPCRSAPPLLYPSLGALLWVAAHSAAAATLTVSPDGPIRRIADA
                     ARLAKDGDTVLIRAGTYRHDVASWPQRNLTIRGVAGRPILDAAGEGAEGKAIWVLKDG
                     HFRIDNIAFRGARVPDGNGAGIRLEHGRLDLSRCVFEDNQMGLLTSDDATVELNIRDS
                     LFRRAPPQRKPLPHLLYAGQIRLLTVQGSRFEQGYEGHLVKSRARRSELKYNLLADGP
                     GGQASYELDLPNGGVAVVVGNVIAQSAATRNRALVSYGAEGALWPENRLLLAHNTLIT
                     TGPQAAWFARAWRERLPADTQVVTRNNLTVGPGVFNLGLGGDHRGDTALTGPAPTPPD
                     YALPAASPLRGRVTAAEQDGEGLPAAEFAWPVGTRPLAAPARWAPGAMQRHASGN"
     gene            complement(7065..7670)
                     /locus_tag="PLH85_06210"
     CDS             complement(7065..7670)
                     /locus_tag="PLH85_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNB64188.1"
                     /translation="MVRKTKEEAETTRRQIIDAARRVFHECGVSRTSLEKIATAAGVT
                     RGAVYWHFKNKTELFFAMRDEAKLPMVDRVVFDPCDENPLGGIETALREMVQVLIDQP
                     ETRETFEIVSFKCEYVDEFALALQGNNCQMDFLDKLAAAYARAAEKGQLRAGFAPEAL
                     AHDTFLFVGGLIKHWLARMPDERFRQRAGDMIRAHVALRRA"
     gene            7817..9013
                     /locus_tag="PLH85_06215"
     CDS             7817..9013
                     /locus_tag="PLH85_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB64189.1"
                     /translation="MTARPNALLTSVPVLLAATLLAACGSKDDKAGGMPPGGMPALPV
                     TVLEMQPTKVETSVEAVAQTEGPREVEVRARVGGILVKRLYDEGAAVKAGQPLFQIDR
                     EPYEVAVAQAKAQVAEAKARVDQTRREAARLKGLLAQQAISQREYDTATSDDAAAAAA
                     VLAAEAALRQAQLNLEYATVTAPVAGLTGRVAVSEGNLLATGGGTLLTTVVQVNPMWV
                     RFSLAEADLAAATPGGRFNPKAVRGVELVLPDGSTYPVRGHLNFSASQVDPKLGTLQL
                     RGEFANPDNVLLPGQFVRARLLTGERDGVFKVPQNAVMQTDMGAVVMLANADNKVEPR
                     PVKTGNWSGKDWVILGGLKAGDKVIVDNLIKIRPGAPVAPHGPGEGPGAPGGQPGAPA
                     AAPAKG"
     gene            9027..12152
                     /locus_tag="PLH85_06220"
     CDS             9027..12152
                     /locus_tag="PLH85_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNB64190.1"
                     /translation="MSRFFISRPIFASVISIIIVIAGLMASLTLPVAQYPEITPPTII
                     ISASYPGANAETLSKTVAAPIEEQLSGAEGLIYYNSQSTSNGTVTITATFEVGTNPDN
                     ALIAVNNRVKVAEPRLPDDVRRTGVLVQKRSNNILMFAAVRSPKGAFDTLYLSNYVAL
                     NIIEEIRRIPGVGDAQQFDAIYAMRVWLKPDVMAKLGITTTDIANAIRVQNTQNAAGK
                     IGQEPVVDGQQLTYTVTAKGRLLTPEEFGGIILKAEGPNGIVRIRDVARIELGAENYD
                     RSTAVNGAPVSGMGIYLQSGANALDTAKRVRARLDEMQKRFPQGMEYFIPYDTTRFIQ
                     ESAHEVVKTLVEAALLVIAVVYLFLQSWRATLIPMVAVPVSLIGTFAGMWLFGFSINT
                     LTLFAMVLAIGIVVDDAIVVLENVERLMSEQKLAPFDAAVEAMREVSGAVVAIVLVLC
                     AVFVPVAFLGGIAGKLYQQFAVTVAVAVVISGIVALTLTPALCALLLKASHEEKPLFK
                     PFNRLFERFTRSYTNTVGLTLRHGIIGTLVFLGTIGATAFLLRIVPGSFVPAEDQGYL
                     FGFVTLTDGASAQRTFAASEQMRTRIMSDDIENIFFVNGIDFITGNNRASVATTFIIF
                     KPWEQRSATADDMAKKFMGVGMSLSDGLGLVFNPPPIQGLGTAGGFEVYLQNRADGDA
                     RRLAQVSGQFLEALRKRPELTGLNSFFRVTAPQLYVEVDEPKALSMGISLDSIYATLQ
                     ATTGTLYVNDFNRAGKTYKVELQAEAAYRMNPEDLLKPYVRTASGAMVPLSAVAKVKT
                     LTGAEMVERFNGFVSAKVLGNAAPGFSSGQAIQAVEEVAREALPEGFQVEWVGQAFQE
                     KRTGSASIVAFTFGIIMVFLILAAQFEKWSLPLAVIMAVPFAMFGALSAVLMRSMPND
                     IYFQIGLVVLVGLAAKNAILIVEFAAQKLDEGMGVVDAAVEAARLRFRPIVMTSLAFV
                     LGVLPLALSTGAGAAARRSMGTGVVGGMLAATFIATIFVPLFFKWLSRKGKGSGRSTP
                     PATAATEEVKS"
     gene            12149..13588
                     /locus_tag="PLH85_06225"
     CDS             12149..13588
                     /locus_tag="PLH85_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNB64191.1"
                     /translation="MSPARPTPALLAVLLAAALGGCAVGPDYVRPESATNLPAQFAPG
                     SAKLAYSPSVASTWWTLFGDRQLDALVGKALANSPDALTAAARVEEADAVIRQVDAAL
                     LPQVDATAAGTHSRISQTTATPLPSSVAAVRNNARLALTTSFELDVWGKLRRASEAAR
                     AQALGSRYGRDTVALSIAGLVTQGYLNLRALDAQRLVTAESVASAERSLKLTQVRFDG
                     GIASQLDVQQADSALATLQAALLQLDQAREQAENLLGYLVGEPGLKIAAGDLRDLPIP
                     PVPPAGLPSSLLEARPDVRQAETDLIAANAKIGVAKAALFPGITLTGNFGRESKDLSQ
                     LFTSPSSVWALGAGITQPIFEGGRLRAQVDQVTAQQKQVAEAYRKAVYTAFKETNDAL
                     ASLRVGADAEDTLTRALNAAKRNLMLAQARYDGGYSPYLEVLTAQRSANDAALAWIQN
                     RQARLAASVDLFRALGGGWQDSQKLAANP"
     assembly_gap    13658..13757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13758..14668)
                     /locus_tag="PLH85_06230"
     CDS             complement(<13758..14668)
                     /locus_tag="PLH85_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNB64192.1"
                     /translation="MTRSARYIVLHETEYTPCGVPVSLAQHLLHLMPRALPWQTVEKA
                     ELTVDPDLARIQQGEDVFGNPVAWMALDTPHDGLLVRAESRVTVLGRTLPKAEESPSW
                     ESVREIFHYHSGARDADSLDAQRYLYSSPRAPRDARLAAFAALAFKPETPLLAGVQAL
                     MEIIYKTFTFDAKATEVSTPVFEVLERRRGVCQDFAHLMIAALRSVGLPARYMSGYLL
                     TDPPPGKPRLVGADASHAWLAVWCPENGWVEYDPTNGIQPDLRHVTLGWGRDFQDVSP
                     LRGVILGGQAQAPEVRVTVMPVDEWEAR"
     gene            complement(14671..17199)
                     /locus_tag="PLH85_06235"
     CDS             complement(14671..17199)
                     /locus_tag="PLH85_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNB64193.1"
                     /translation="MPSSILSHYPYRTDAYDELFGPDGVRPHWQDFLDHVDESSADTL
                     KARAAQLARGIQQNGVTYNVYADARGTDRPWSLDLLPLILPADEWREISTAVAQRATL
                     LNALLADLYGPQTTLAEGLLPPELVYGHKGFLWPCQGIQPPGGTYLHLYAVDLARSPD
                     GRWWVIADRTQSPSGAGYALENRLLVSRAFPDLFRDLQVQTLAAFFRSLQRSLAAHAP
                     CDGNQVPLTVLLTPGPFNETYFEHVYLARYLGFPLVEGQDLTVREDTVYLKTVSGLKR
                     VHAILRRLDDDFCDPVELRADSALGVPGLLGAVRAGRVLVANALGSGLLESAALPGFL
                     PGICRHLLKEELAMPAVATWWCGEDPALDHVLGHLDSLVIKPTFPGGLSEVVFGDQLD
                     EDQREALRVRIAANPSAWVAQELVQLSGAPAWAGGHRRRLINRSMGLRVYAAASPEGY
                     TVLPGGLTRVAGMENARLLTMQKGGSSKDTWVLSDGPVSTFSMLPKNLRVADLVKVTG
                     TLSSRLAENLFWFGRYSERCDAVARLARLTLARLSEGRRTERSAALAMLLELAAGAEL
                     VERDVVRTSLGKAELALFRAVLDRRAGNGLAANLQRLTWCATQAREHLSLDHWHALKR
                     LSAEIEDCRGRVSDTSALLEFLDEALTTFVSLSGFAMDNMTRDMGWCLHILGRRIERL
                     DSLGSTLAHFLRCHADTAQPLQREMAVEALLELCDSIITYRTRYRAQPELLPAIHLLV
                     MDDANPHALAFQVRMIINYLGKYEQDGDGIGIAGLEEALYVIEHFSWESLERVRPDAE
                     RAAASLALAHALDALVHAGRELSDRLAMRFFSHVGRGSQATFAA"
     gene            complement(17342..20731)
                     /locus_tag="PLH85_06240"
     CDS             complement(17342..20731)
                     /locus_tag="PLH85_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNB64194.1"
                     /translation="MTIHVALNHLTSYKYDRPVTLGPQVVRLRPAPHSRTRILAYSLK
                     VLPKPHFINWQQDPQSNYLARLVFPEKVTEFTVEVDLVAEMSVINPFDFFLEPEAENY
                     PFEYAGNLSHELAPFRLKDAPTPLFQAYLDSVPRDSRRVVDFLVDINAKLQQHIGYVI
                     RMEPGVQAPEETLEKRTGSCRDSAWLLVQLLRHLGFAARFVSGYLIQLKADVKALDGP
                     SGTEVDFTDLHAWTEVYLPGAGWVGLDPTSGLFAGEGHIPLAASPDPTSAAPITGLVD
                     ECECEFAHHMSVTRVLEAPRVTKPYTEEQWAEIEALGHVIDEDLKAHDMRLTMGGEPT
                     FVSIDDPDGAEWNTEAMGPTKRVRAEDLYHRLRDKYGPTGLAHFGQGKWYPGEQLPRW
                     SLNCYWRKDGEPIWTHPDLIADSATPGDADADLAGAFLRDVAERLGLDARRVFAGYED
                     AFYYLWRERRLPGNVDPFDARLDDPLERDRLRRVFDYGLANAVGYVLPISINTYTGAW
                     QTGPWFLRDERCYLIPGDSPIGYRLPLDSQPWVTATSYPYVNPPDAHQSFPPLPEYQA
                     IRRQLPGGQTAVLNALNAFKDDETYGLGSGAAARAAALKAARAAEKLSPTDRTPQSFE
                     SADWIVRTAMCVEPRNGTLHIFMPPLSRLENYLELVAAVESSAEAFGTPVVLEGYEPP
                     TDPRLTHFRITPDPGVIEVNIHPAGSWEELVDRTTHLYDAAHFSRLTTEKFMVDGRHT
                     GTGGGNHFVLGGATPSDSPFLRRPDLLRSLISYWHNHPSLSYLFSGLFIGPTSQAPRI
                     DEARNDSVYEIETAFRQFPKVGDSVPPWLIDRMLRNLLIDVTGNTHRAEFCIDKLYSP
                     DGPAGRLGLVEMRAFEMPPHARMSLAQQLLLRGLLARFWDTPYAPERLVRWGTELHDR
                     FLLPHFVWQDFSDVIEDMQGHGLDFKPEWFAPHQEFRFPKIGDFAARGIEVELRGALE
                     PWHVMGEEGAIGGTVRFVDSSVERMQVKVSGMSDDRYLLTANGRSIPLQPTGTVGEFV
                     AGVRYRAWQPPSCLHPTIPSHAPLVFDLVDTWTGRSLGGCQYHVSHPGGRSFETFPIN
                     AFEAESRRLARFFRMGHTPGAPKFETSAVEKEFPFTLDLRR"
     gene            complement(20990..22507)
                     /locus_tag="PLH85_06245"
     CDS             complement(20990..22507)
                     /locus_tag="PLH85_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNB64195.1"
                     /translation="MTEPLLIAKSGPKDIVLLPALANRHGLITGATGTGKTVTLQKMA
                     ESFAAIGVPVFMADVKGDLSGIGAASNVSPKLKERLTALGIDDFTPRANTAVFWDVFG
                     TSGHPVRATVSDMGPLLLARLLNLNDTQAGVLQLVFKIADDQGLLIIDLKDLRATLQY
                     VGDNAKDFTTQYGNISAASIGAIQRGLMTIEAQGGDKFFGEPMLDIADLIQTDGDGRG
                     VVNILAADALYNSPKLYSTFLLWLLAELFEHLPEVGDLDKPKLVFFFDEAHLLFNEAP
                     KALLEKIEQVVRLIRSKGVGVYFVTQSPLDIPENVLGQLGNRVQHALRAFSPKDQKAV
                     KTAAETLRANPEFDAATVIMELGVGEALVSFLDEKGRPSIVERGFIVPPASRLGPLSG
                     EERKAAIENSVIFGHYEQIVDRESAFEKLRGAPAAQAAPAGAPGGASAPAPAQDGGGL
                     LGGLGDILFGTTGPRGGRHPGLAETAARSVVRTIGSQVGREIVRGVLGSILGGRR"
     gene            22714..23442
                     /locus_tag="PLH85_06250"
     CDS             22714..23442
                     /locus_tag="PLH85_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNB64196.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGRVFSRLAKEITVAARMGGGDPG
                     FNPRLRLAVDRAKAVNMPADNIDRAVKKGTGELEGVTYEEARYEGYGIGGAAVMVDCL
                     TDNKTRTVADVRHAFNKFGGNMGTDGCVAFQFKHCGQLLFAPGTSEDALMEAALEAGA
                     DDVVSNEDGSIEVLTEPYQFGEVKDALEKAGFTPEFGEVTMKPLNEAELTGDDAVRMQ
                     KLLDALESLDDVQAVYTSAVMDDA"
     gene            complement(23530..24474)
                     /locus_tag="PLH85_06255"
     CDS             complement(23530..24474)
                     /locus_tag="PLH85_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB64197.1"
                     /translation="MTHAPAFAENSSSRESVHARPSRVVTGAGGAGHQPCILFADVAG
                     GVRLAAKLGDAEAGYAVERCLNRMSRCAEAYATSGIEVRPDVLVARFADADAALYAAR
                     EMRERVRALPPMSGIKLVLRAGLVLESEAPDDLKGAETLASRLAVGHNPDTVSVSEAF
                     SEALSPAMRKLLSSMVQANDQLPFQVLELGEATPPSPVELRHASVRPKAQMKVSFQGR
                     SWTVDASSPTLLFGRETGNDIVVVDPRVSRQHARIELRNGLFYLTDSSTNGTYLLEEG
                     AAEHCIKRDDFILGEKGYIGCGFSVADNMSGAVAFALG"
     gene            complement(24471..25415)
                     /gene="ttcA"
                     /locus_tag="PLH85_06260"
     CDS             complement(24471..25415)
                     /gene="ttcA"
                     /locus_tag="PLH85_06260"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNB64198.1"
                     /translation="MDLAVGAALPLPIDTPTPESRTSNTFRNLKRRLERKVGEAIADY
                     GMIRDGDVVMVCVSGGKDSFSLLSILLALQKRAPVNFRIVAMNLDQKQPGFPDHVLPA
                     YFESIGVEYRIVTENTYKIVKDKIPEGKTTCSLCSRLRRGIIYRTAKELGATKIALGH
                     HRDDMIETLFLNMFFGGKMKAMPPKLVSDDGQNVVIRPLAYCPEKDIERFARGMGYPI
                     IPCNLCGSQENAQRKQIKAMLRDWDARFPGRIVSIATALQNVVPSHLADGKLFDFLNV
                     SCDTKVDQGDLVFDAPELPSVPPAEAAVSLGFLREKSP"
     gene            complement(25482..26231)
                     /locus_tag="PLH85_06265"
     CDS             complement(25482..26231)
                     /locus_tag="PLH85_06265"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNB64199.1"
                     /translation="MSSASPAPVMLVTGGARRVGAAIVRRLHGEGARVAVHCRHSRDE
                     AEALAAELNARRPGSVAVLQADLLDTAVLPALVSAVVARFGRLDGLVNNASSFFPTPM
                     GRMDEAAWTDLVGSNLKAPLFLSQAAAPHLLATRGSIVNIVDVHAERPLHGYALYCAA
                     KAGLLGLTRALAVELGPAVRVNGVAPGAIEWPDDGQFPPAAQDAVVRHTLLGRVGEPD
                     DVARTVVFLLCNAPYITGQIIAVDGGRSAHL"
     gene            26365..27525
                     /locus_tag="PLH85_06270"
     CDS             26365..27525
                     /locus_tag="PLH85_06270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB64200.1"
                     /translation="MNLPPPSPDALAQSERLVARLRDLIAREGGWISFATYMGEALYA
                     PGLGYYSGGSRKFGPGGDFITAPELTPLFGQAVAGQLVEIMAQSAPQLIEAGAGTGLL
                     AADLLLELEHRGALPERYAILEVSGELRSRQFDTLAEKVPHLASRVCWLDTLPEHFSG
                     ALIANEVLDVMPVHLVAWRPEGLFERGVAMDDAGRFVWADVPASGRVAEAAAALERPL
                     PSTGEYVSELNLASRAWIGEWAARLDKGALLLVDYGYPRAEYYLDSRSTGTLLCYYRH
                     RAHPDPFLWPGLNDITAFVDFTAMAEAGFDAGLDVLGYTSQATLLLNCGVLECLARRA
                     PEDSPDYIRASRAVQRLTGPHEMGELFKVIALGRGIDGPLLGFRRGDRLHTL"
     gene            complement(27578..27796)
                     /locus_tag="PLH85_06275"
     CDS             complement(27578..27796)
                     /locus_tag="PLH85_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64201.1"
                     /translation="MVIYMDMESGAQARMDPVDEQAPEYGDEILNANLLPPELALGLQ
                     EVAVTHAERHFVPTDVDAFLSAIYRFQE"
     gene            complement(27992..28795)
                     /locus_tag="PLH85_06280"
     CDS             complement(27992..28795)
                     /locus_tag="PLH85_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB64202.1"
                     /translation="MKSARFGDLEVLCRLPSGTPRPTPLLFVHGAFTAAWCWEEHFLP
                     HFASLGYAAYAVSLSGHGSSRNRAFLDSYSLADYVADVADVADRLPAPPVLIGHSMGG
                     MVVQKCLERQRVPAAVLMAAVPPQGLLGSAFGLAFSRPNMMGDLNRIMGGGQPDLDTL
                     RDALFHEPVELVRLRQYYHLCQPESHRAIWDMSLFDLPALGHVALPPMLVLGAEHDML
                     IPPDQVRMTARRYGTEAYIVPRLGHGMMLEAGWRIAADPIAAWLAAHGL"
     gene            complement(29066..29476)
                     /locus_tag="PLH85_06285"
     CDS             complement(29066..29476)
                     /locus_tag="PLH85_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64203.1"
                     /translation="MEDTMKPWVQGLLLGLCASGALAADPPQCRQQIVEKSTLELCLV
                     PGAALQHDLYVLKADRQVIFALVDDFAEKVELEHEIPEGLVVEFPLSLQGEKIVKISG
                     GCRPESKEGLEVARVCNFHWGKHHVVKDVRFEFK"
     gene            29561..29857
                     /locus_tag="PLH85_06290"
     CDS             29561..29857
                     /locus_tag="PLH85_06290"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNB64204.1"
                     /translation="MLGARRRIPLSKVLPRSGRLRFNTAFVLPVPRPMLKWLLAIVVI
                     AVVSGLGRGISPGRWRIGHLPGDLHFHLWGRHIHIPFTSTLLLSLAAMLVIRLL"
     gene            complement(29854..31920)
                     /locus_tag="PLH85_06295"
     CDS             complement(29854..31920)
                     /locus_tag="PLH85_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNB64205.1"
                     /translation="MRLLRVPSLPGLRPGRLSGPRAGVRPRRATGPETRIGAVLLAAI
                     LALPALPARAHQPLPAFADVKAAARPSVAVLLARDGTPLAERRLDPQVRRLEWVGLPA
                     LSPVLKEALLAAEDKRFFEHSGVDWQAFAGSLWHNLWNSRKRGASTLSMQLAGLLDPE
                     LALGRDGQPRRSLGQKWDQAFAAQALEARWTKDQILEAYLNLAPFRGDLQGVHAAARA
                     FFGKTPGELNRPESLILAVLLRGPNARPEVVGHRACMLAERLGTPAACASAQRLAQNT
                     LDRQQLQPRWDLAPHAAAQLLHQPGERIVSTLDADAQKLVLAALRRSGVSEAAAVVLD
                     NASGDVIAYVGALEPAAADALPVPRTSASLLAPLVYGLALERRVLTAATLLEDALSNH
                     APTLPDEKLWVSARRALADGLPEPAAAVQRLVAEALPERLRVMELDPARGPAPDLSLL
                     QLAGFYRSLANGGLWQEVRLTQGERAAPRRLLRSEANFIVGDMLVLDDAEGVPQPFVQ
                     RMASGDGRDMEVAGFAERYTLVLWAAGGRWTASSAGAAWQEILRGLYRGTVPARTVAA
                     PVGVVSSVVVFDPPVEPARREWFIRGTETDKVGMPRPAAHILHPAAGSLVDGMALADD
                     PAFRIRFEASPALPGLSWRLDGERIGRGAHLAWTPVAGLHTLELLAADDRVLDTVTFA
                     VRAPLP"
     gene            complement(31966..33246)
                     /locus_tag="PLH85_06300"
     CDS             complement(31966..33246)
                     /locus_tag="PLH85_06300"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNB64206.1"
                     /translation="MKVYCVGGAIRDELLGLPVQDRDWVVVGATPEDLLARGFRPVGK
                     DFPVFLHPASHEEYALARTERKTAPGYAGFVFHTDPSVSLEEDLARRDLTINAMARDE
                     AGSLMDPYGGQADLAARVFRHVSPAFAEDPVRILRLARFAARFADFRVAPETLVLMRR
                     MVDDGEVDALVPERVWQELARGLMEARPSRMLRVLRDCGALARLLPEVDRLFGVPQPA
                     KHHPEVDTGEHLCLVLDHAAATGQPLPVRFACLLHDLGKGVTPAPVLPHHYGHEASSE
                     RLARAVCERLKCPVDCRDLALMTAREHGIVHRALELRPDTTVKLIERCDGLRRPERFV
                     ALLDACACDHAGRAGLAGRPYPARDRLLAALAAVRAVDAGAIARQQADKARIPAAVHA
                     ARIDAVKALGRGTEVPVGAANDTQIDSPDDAGVT"
     gene            complement(33243..34196)
                     /locus_tag="PLH85_06305"
     CDS             complement(33243..34196)
                     /locus_tag="PLH85_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNB64207.1"
                     /translation="MTSRKVLIVGGSGFVGTAVAARLARDGLSLRIPTRRRARVRHLL
                     TLPDVDLVEADVFDPPTLAGLMQGVDAVVSLVGVLHSRPGQPYGPDFARAHVELPTLL
                     VAAARQAGVTRLVHVSALGAAPDGPSEYQRSKAAGEAAIRAGAPELAWTILRPSVIFG
                     PGDSFLNLFAGLVRTFPVLPLGGAGARFQPVYVQDVADVVAEALVRPDAAGLTCDLAG
                     PTVYTLAELVAYVARLTGRSTRIVPLSEPLALLQAAVLERLPGPLMSRDNVRSMRADN
                     ITGGPPQPWGRTPTALETVVPRWLGRANRRGHFDAFRKPRP"
     gene            complement(34273..36240)
                     /locus_tag="PLH85_06310"
     CDS             complement(34273..36240)
                     /locus_tag="PLH85_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB64208.1"
                     /translation="MKNSVRAAIAAVLWAVLAAQADTGDERILAARDAARSGDRARLA
                     AYAAPAGHVLDPYPEYWWLSAQVARPADIDPAALREFLERNRGAWLAEKLRGEWLRGL
                     GRRGEWGTFAIEYPFMEQPEQDLQCYQFQARLANGDGMALAEARPLWFNLVDTPESCV
                     PVLQTLVREALVSPDEIWARVRRLMEAKRNTHARYAASWLPADQVPAPSDLDKAAANP
                     AGYLDRLPPNFSATRPGRELALIALARLARDDTLGAYVRYQRMDERFSPAERGYALGQ
                     IAWQAAQRLMPEAHTWYRAAGDTPMSDEQIAWRARAALRAGDWKGVRSAIEAMPASLR
                     EQPDWAYWLGRAQAALGKQDAARRLFEQIGGHPAFYSILADEELGRSFVLPKKARGPS
                     DDEATLVRTTPGVQRAVALFRLDMRTEGTREWNWSMRNRDDRFLLAAAAYAQELGIYD
                     RAINAADRTRSEHDYSLRYLAPYRERIEPNARSRDLDTAWVYGLMRQESRFVTAARSG
                     VGAQGLMQIMPSTGQWIAGKLGMKGYNIGWLRDPDTNVMLGTTYMRMVLEGLDNHPVL
                     ASAAYNAGPGRARKWRDSKPLEGAIYAETIPFNETRDYVKKVMANSIVYAALLDGRAP
                     SLKARLGTVLPGNGDASGLGSPAPGAGLENE"
BASE COUNT         5738 a        12191 c        13137 g         5199 t
ORIGIN      
        1 cggctgacga tccaggccag ctcttcggcg gtgtagaact ccaggcggga gacgatgccg
       61 aagcggtcgc gcagggggtt ggtgagcatg ccggcgcggg tggtggcgcc caccagggtg
      121 aagggcggca gatccagctt gaccgagcgg gccgccggac cttcgccgat catgatgtcg
      181 atctggaaat cctccagggc cgggtagagg atctcctcca ccaccgggct gaggcggtgg
      241 atctcgtcga tgaacaggac gtcgtggggt tccaggttgg tgaggatcgc cgccagatcg
      301 cccgcccgct ccagcaccgg gccggaggtc tggcgcaggt tgacgcccat ctccgccgcc
      361 acgatgtggg ccagggtggt cttgcccagg cccggcgggc cgaacaggag cacgtggtcg
      421 agggcctcgc tgcggcgccg cgcggcctgg atgaagatct ccagctggtc gcggatcttc
      481 tgctggccca cgtaatccgc cagccgcttg ggccgcaggg cgcgctccag ggcgtcttcc
      541 tgggcgccgt tgctgcgggc gccgatcagc cgcgcggccg gatcgggcgc gccgcccttg
      601 agcttgtcgg tctcgatcat gcctcgtcct ccgcggccgg cgccgcgatc tgcggctcga
      661 tggcggtcca ttcccgcacc agcgcctgcc ccagggccag gaaggtgggg ccgaggaaca
      721 ggccgatgaa gccgaaggcc agcacgccac cgaaggcgcc cagggcgatc agcaggatgg
      781 gcaggctggc ggtgcggctg atcagcatcg gcttgaggaa gttgtccacc gaactgaccg
      841 ccagggtgcc ccacagcacc atgaagatcg cccagccgct ctggtcctgc tgatacagcc
      901 agaaggccgc gccgccccag atcagcggcg gcccgacggg caccatggac aggaagaaga
      961 tccccgccgc gagcagcagc gcccccggca cgccggccac cagaaagccc gccagcgcca
     1021 ccagggcctg ggccgccgcg gtgccgacga tgccgatcat caccgccttc acggtgttgc
     1081 cggccagttc cagcaggcgc tcgccgagct cgccccccag gcgcagggcg ccggtgtcga
     1141 gcatgcgggc caggcgttcg ccgtcccggt acaggaagaa cacgatgaac agcaccaggg
     1201 agatctgcaa cagcccctgg cccaccaccc gcgcggcgcc cagggcaaac ttgcgcgccg
     1261 gctccagcgc ctgcagggcc agcttgcgtg cctcctcgtg gttgcgggtg aggcggcgca
     1321 cgtacacatc cacgtccggc cccaccacgg gaatgtcctt gagccacgcc ggcggcttgc
     1381 cgtcggactg ctcgatggcc tcccgcgcct gatccacgcc ctccgccacc gcatccgtga
     1441 gggacgccgt cagcccgatc atgggcagca gcagcaccgc cgtgagcagc accgtcatgg
     1501 agatcgccgc cagcccgtca cgccgcccca gccgccgatg cagggcggcg tacatgggcc
     1561 aggtggtgat gcacaccgtc ccggcaaaca ggatcgccgc aaagaacggc gccagcacaa
     1621 aggcacagcc cagcagcagc agggcgatga tggcgatctg ggcggtacgt cggggagcca
     1681 tggggatacg ggggaatggg gaggagggat tatcgctgaa tcgggtgacg gatcggggac
     1741 ggcgatcggc caccgcgcag ccgcacgcgc ccccgcctaa tccgccggcc tgcgcctcac
     1801 gtacaccgtc ttttccaccg tcgcgaccac ctcgccgcgg cggttgagga cgtccacctc
     1861 gaacaccggc tccaccttgt cgccgccggc ggtggcgtgg cggatcgcgt ccacctgcgc
     1921 aggcgggacg cggaaggtgg cgtacacggt gccgcggccg gggagcttgt agtcgatgcg
     1981 cgaggccttg tcccacacca cgtagccacg gccgagctgg tacatgacca gcagggcgaa
     2041 gaaggggtcg gtcatggcgt acagggagcc accgaagtgg gtgcccatgg cgttgcggtt
     2101 gagcaggccc aggcgcaggc gcacgacggc ttcggtgaag tccggggcga tgcgttccac
     2161 gtggatgccg gcgccgatga agggcggcca caggttcatg agcaggcgca ggctgccggc
     2221 gccgatctgc acgttgcgca ggaagccctg gcggagcttg cggctcatgc cttggagagg
     2281 cccttgaggg cctggcggat gccgtcggag acgccgacgc cttcgggcag ggtcttgacc
     2341 gcggcgaggg cttccttctc gttgtagcca agggcgagga gggcgtggac gatgtcgctg
     2401 cgtccgtcgc ccggcgcggc ggtggcgagc ccgccggcgg cggcgaggcc gggcagggcc
     2461 ttgggcagct tgtcgcgcag ctccagcagc aggcgttcgg cggtcttctt gccgatgccg
     2521 gggatcttga tgaggcggcc ggattcctgc agggcgatgg cctgggcgag gtcgctgacg
     2581 gacaggccgg agagcacggc cagcgcggtg cgggccccca cgccggccac cttgaggagc
     2641 tggcggaagg cggcgcgctc gtcctcgctg gcaaagccgt agaggaagtg gccgtcctcg
     2701 cgcaccgcca ggtgggtggc gagggtgacg gtctggccgg tggcgggcag gccgtagaag
     2761 gtgctcatgg gcacttccag ctcgtagccg acgccgttca cgtcgaggag gatctggggc
     2821 ggggcttttt cggcgaggcg tccggtgagg cgtccgatca tggggcgttc aggtcaggtg
     2881 aagtcggcgg ccacgaaggc cggcagggtt tcggcatcgg cggcgagggc cgccacgtgg
     2941 gggtagtcgc cgtcggcgag gaagccgggg gcgctgtaat gggtgaagcg ccaggccacg
     3001 gcgacggtga tgtcggcctg ggtgggggcg tcgccgcaca gccagtcggc gtcgccgacg
     3061 cgggcgtcga tcatggccca ggtgtcgcgg agctggcgct cggtgcgcgc cagccattcc
     3121 ggaaaacggg cctccttggg ccggatgcgg gtctcgagca cgcgatggag ggccttttcg
     3181 gcggcggcaa ggccaaagcc ggtgagctgc aggcagcgcc gacggtcggc gggcgcggca
     3241 ggcagcaggc tgctgccggg cgcggcgagg gattcgaaga gggtgatgat ctggctgctg
     3301 tccaccatca cctcgccgtc gtcgaagacc agggtgggcg ccttgaccag cgggttgagg
     3361 gcgcggaatt cctcgacctg gcgcagcacc gacagggact ggtgctcgaa ggggatgccc
     3421 agcatgcggc cactgatgaa ggcgcgacgg acaaaggggg aatcgagcat gccgacgagt
     3481 ttcatggaga cctccggtca gggcggggtg ggggaaggga aacgacaggc ggacgcggtg
     3541 cgacgcaggc ggtcgaggac ggcctcgcct tcttcgcggg aacggatcgg gatgcgcgcg
     3601 tcgtggccgc tgcccaggtt gccgccgccg taggcgaggc gcacttcgtc ggcgcggacc
     3661 tccacgtact gcacgatgcc caggttgatg aagcgctggg cgccggcctc ggccggtagc
     3721 gggaacaggc agaaggcggt ggagccgggt gcctgggcgg ccgcgcaggc gggcagcccg
     3781 aatgccagca gggcggcgcg gacgcgtctc atggggcgcg cccgaagaag tcggccagat
     3841 cggcgaggct cgcggccacc ggcacggcgg cggcgcgcac gtcgtcggcg ggctggagca
     3901 ggtccggcac catcaccacc cgcgccccgg cggcgagggc gccgcgcacg ccggcgttgg
     3961 aatcctccag caccaggcag cgggccggcg gcaggcggag ccgcgcggcg gcggccagga
     4021 agatgtccgg cgcgggcttg ccgcggctca cctcgtcgcc gcacaccatg ccgtgcaggc
     4081 gcggcagcag gcccaccgca gcgagcttgg gctcggcccg cacgcggcgc gtggaggtgg
     4141 ccaccacgcg gcccaggccg agggtgtcga gcacgtcgaa gagggcctcc acgccgggct
     4201 tgagggggat gcgccctgcg gcgatggccc gttcgtagtg cacgccgcag gcatccatca
     4261 gggcctccac ggggaagtct gcgcccaggt ggctgcggac aacggcgggg gaatcccgcg
     4321 agttgagccc caccatggcg agcccgacct cgtgacgcca gggcaggccc agttcggccg
     4381 cagtggcttc gatgatggtc agggcgaccc gctcactgtc cagcagcagg ccgtccatgt
     4441 cgaagatgac gccgtcgatg tcgcccggca ggaagggcag ggacgggctc atgcgccgcc
     4501 tcccggccag cgcgcgaagg ccagcgtggc ggcgaggatc agcccgtgca ggtgagccgc
     4561 ggcgatggtg agcttgaggg caggcgccag ggcggccggc tcggcagcgt gggcgagcag
     4621 gtggcgcagg gcacggaagg acaggatcag gctgaaagcc gcggccgccg ccgcgtgggg
     4681 cagcagctcg cgccccacca tcagcatcag ccaggcgtag gcgcccacca cgaggccgac
     4741 gcaggcccac ttggcgccat ccggcccgaa gcggaccacc agggtgcgct tgccggcggc
     4801 ggcatcgccg cggtggtccg ggaattcgtt gaagaacagg atcgccgcca ccagcagcgc
     4861 gtaggacagc cctgccgcca ccggcaggaa cgaccaggcg ccgcgctgca cgaagtcgct
     4921 gcccgccacc acgatgagcc agcccgccgc cacggcgatt tctcccaggc cgcggctcac
     4981 cagggcaagg ggcggcgcgg tgtaggccca gcccaggaac aggccggcga cacccagggc
     5041 gaccaggccg atgccggact gggccgccag ccacagcccg gcaggcacca ccggcagcag
     5101 caggccccag cccaggcgcg cggtctcggc ctcggagagc acgccgttct ggatgaagcg
     5161 gctgccgccg gtaaagggaa agacccgctc cgtgttggcc gcgtcggcac cgctgcgggc
     5221 atcgtaatag tcgttgagga cgttgatgcc ggcgtgggcg aagagggcga aggcgatggt
     5281 cagcgccgcg cgggtggaat ccacgatcac gccgctgccg tgggcagtgg ccagccccac
     5341 caggcaggcg cacagggtga cgccgagaaa ggccggccgg gtggcgagaa aggccgccag
     5401 gacggggcgg gtgcgcagga cgacgcgggg ttcggcagga gacgcgggag gagcggacgc
     5461 catcgggaca gcatgaaggt gggaagcccc catcctgcga cgggccgggg cgccggtcaa
     5521 gggcgcggcg ccggacggac agatggcggg ccgggccgtg cacacgccac ggggacgcgg
     5581 ccgggcagga accgacaagg cacgcgcatc ccgccggcgg ggccctccgg ccggcgacgg
     5641 cggacagtca atggcggggt cccgcgccgc cgcgcccgat gcccgagcgc cgcgggcttg
     5701 cgcagccggc cgccacgcag gctcacgttt gtgcgcacca gggcgcaggg gcgcaggcgt
     5761 accatcgcga tcgacaccca ccgtgaccgg gacgcctgtt ccgcccggtg gccccttcca
     5821 ccggccgacc ccgtgggacg cgacggcgtc ccccaatcgc ccatttccct tttccgaccc
     5881 gatgccttgt cgatccgccc cgcccctgct gtatccaagc ctcggtgccc tgctgtgggt
     5941 cgccgcccac agtgccgccg cggccaccct cacggtgagc ccggacgggc cgatccgccg
     6001 catcgccgac gcggcacgcc tggccaaaga cggtgacacg gtgctgatcc gcgccggcac
     6061 ctaccgccac gacgtggcca gctggcccca gcggaacctg acgatccgcg gcgtggccgg
     6121 ccggcccatc ctggacgcgg cgggcgaggg cgccgaaggc aaggcgatct gggtgctcaa
     6181 ggacgggcat ttccgcatcg acaacatcgc attccgcggc gcccgcgtgc cggacggcaa
     6241 cggcgccggc atccgcctgg aacacggccg cctggacctg agccgctgcg tcttcgagga
     6301 caaccagatg gggctgctca cctcggacga cgccacggtg gaactgaaca tccgcgacag
     6361 cctgttccgc cgcgcgcccc cccagcgcaa gccgctgccg cacctgctct acgccggcca
     6421 gatccgcctg ctgacggtgc agggcagccg cttcgagcag ggctacgagg gccacctggt
     6481 gaagagccgc gcccgccgca gcgagctgaa gtacaacctg ctcgccgacg gccccggcgg
     6541 tcaggcctcg tacgaactgg atctgcccaa tggcggcgtg gcggtggtgg tgggcaacgt
     6601 gatcgcccag agcgccgcga cgcgcaaccg ggccctcgtc tcctacggcg ccgaaggcgc
     6661 gctgtggccg gagaaccgcc tgctgctggc ccacaacaca ctgatcacca ccggcccgca
     6721 ggcggcgtgg ttcgcccgcg cctggcgcga acgcctgccg gcggacaccc aggtcgtgac
     6781 ccgcaacaac ctgacggtcg gtccgggcgt cttcaatctg ggcctggggg gcgatcatcg
     6841 cggtgacacg gcgctgaccg ggccggcgcc gacaccgccg gactacgccc tgcccgccgc
     6901 ttcgcccctg cgcggacggg ttacggcggc ggagcaggac ggcgagggtc tgccggcagc
     6961 ggaattcgca tggcccgtgg gcacccggcc gctggccgca ccggcccgct gggcaccggg
     7021 ggccatgcag cgccacgcct ccggcaactg agccggcacc gcgctcaggc ccggcgcagg
     7081 gccacgtggg cgcggatcat gtcgccggcc cgctggcgga aacgctcgtc gggcatgcgc
     7141 gcgagccagt gcttgatgag cccgccgacg aacaggaagg tatcgtgagc cagggcttcc
     7201 ggcgcaaaac cggcgcgcaa ctggcccttc tcggcagcgc gggcgtaggc ggcggcgagt
     7261 ttgtcgagga aatccatctg gcagttgttg ccctgcaggg cgagggcgaa ctcgtccacg
     7321 tactcgcact tgaagctgac gatctcgaag gtctcacggg tttccggctg gtcgatgagc
     7381 acctgcacca tctcccgcag ggcggtctcg atgccgccga ggggattctc gtcgcaggga
     7441 tcgaagacca cccggtccac catgggcagc ttggcctcgt cgcgcatggc gaagaacagc
     7501 tcggtcttgt tcttgaaatg ccagtacacg gcgccccgcg tcaccccagc ggcggtggcg
     7561 atcttctcca gcgaggtgcg gctcaccccg cactcgtgaa acacccggcg cgcagcatcg
     7621 atgatctggc ggcgggtggt ttcggcttct tccttggttt tgcggaccat atgggatact
     7681 ccgacagcat gggtcgggca gcaggctgca tccactgcag acgcagcgac aaatgtagcg
     7741 aaaggtattt acatacattc gtgtttgtat aaactatgac gcaaactgtc ccaaaaatcc
     7801 agtcccggag gtccccatga ccgcgcgccc caacgccctg ctcacgtccg tgcccgtcct
     7861 gcttgccgcc acgctcctcg ccgcctgcgg cagcaaggac gacaaggccg gcgggatgcc
     7921 ccccggcggc atgcccgccc tgcccgtcac cgtcctcgag atgcagccca ccaaggtgga
     7981 gaccagcgtc gaggcggttg cccagaccga aggcccgcgc gaagtggaag tgcgcgcccg
     8041 cgtcggcggc atcctcgtga agcgcctgta cgacgaaggc gcggcggtga aggctggcca
     8101 gcccctcttc cagatcgacc gggagcccta cgaggtggcc gtggcccagg ccaaggccca
     8161 ggtcgccgag gccaaggccc gcgtcgacca gacccgccgc gaagccgccc gcctgaaggg
     8221 cctcctcgcc cagcaggcca tcagccagcg cgaatacgac accgccacct ctgacgatgc
     8281 cgccgctgcg gccgccgtgc tggccgccga ggcagccctg cgccaggccc agctcaatct
     8341 ggaatacgcc accgtcaccg cgccggtggc cggcctcacc ggccgcgtcg cggtgtcgga
     8401 aggcaatctg ctggccaccg gcggcgggac gctgctgacc acggtggtcc aggtgaatcc
     8461 catgtgggtg cgcttctccc tggcggaggc cgacctggcc gccgccacgc cgggcggacg
     8521 cttcaacccc aaggccgtgc gcggcgtgga actggtcctg ccggacggca gcacctaccc
     8581 ggtgcgcggc catctgaact tctccgccag ccaggtggat cccaagctcg gcaccctgca
     8641 gctgcgcggc gagttcgcca acccggacaa cgtgctgctg cccggccagt tcgtgcgcgc
     8701 gcgcctgctc accggcgagc gcgacggcgt cttcaaggtg ccccagaacg ccgtgatgca
     8761 gacggacatg ggcgccgtgg tgatgctcgc caacgccgac aacaaggtgg agccgcggcc
     8821 ggtcaagacc ggcaactgga gtggcaagga ctgggtgatc ctgggcggcc tgaaggccgg
     8881 cgacaaggtc attgtggaca acctgatcaa gatccgcccc ggtgcgccgg tggcgcccca
     8941 cgggccgggc gaaggtccgg gcgcgccggg cggccagccg ggtgcgcctg ccgccgcgcc
     9001 ggccaagggc tgaggaggcg catcccatgt cacgcttctt catcagtcgc ccgatcttcg
     9061 cgtcggtcat ctccatcatc atcgtgatcg ccggcctgat ggcctccctc acgctgccgg
     9121 tggcccagta ccccgagatc acgccgccca ccatcatcat cagcgccagc taccccggcg
     9181 ccaacgccga aaccctgtcc aagacggtgg ccgcgcccat cgaggagcag ctctccggcg
     9241 ccgaggggct gatctactac aactcccaga gcacctccaa cggcacggtg accatcaccg
     9301 ccaccttcga ggtgggcacc aacccggaca acgccctcat cgcggtgaac aaccgggtca
     9361 aggtggccga gccgcgcctg cccgacgacg tgcgccgcac cggcgtgctg gtgcagaagc
     9421 gctccaacaa catcctgatg ttcgccgcgg tgcgctcccc caagggcgcg ttcgacacgc
     9481 tctacctgtc caactacgta gccctcaaca tcatcgagga gatccgccgc attcccggtg
     9541 tgggcgacgc ccagcagttc gacgcgatct acgccatgcg ggtgtggctg aagccggacg
     9601 tgatggccaa gctgggcatc accaccaccg acatcgccaa cgcgatccgg gtgcagaaca
     9661 cccagaacgc cgccggcaag atcggccagg agccggtggt ggatggccag cagctcacct
     9721 acacggtgac ggccaagggt cgcctgctca cgcccgagga gttcggcggg atcatcctca
     9781 aggccgaagg ccccaacggc atcgtgcgca tccgcgacgt ggcgcgcatc gagctgggcg
     9841 ccgagaacta cgaccgctcc accgcggtga acggcgcgcc ggtgtcgggc atgggcatct
     9901 acctgcagtc cggcgccaac gccctcgaca cggccaagcg cgtgcgcgcg cgcctggacg
     9961 agatgcagaa gcgcttcccg cagggcatgg aatacttcat cccctacgac accacccgct
    10021 tcatccagga gtccgcccac gaggtggtca agaccctggt ggaggcggcc ctgctggtga
    10081 tcgcggtggt ctacctgttc ctgcagagct ggcgcgccac cctgatcccg atggtggcgg
    10141 tgccggtgtc gctgatcggc accttcgccg ggatgtggct gttcggcttc tccatcaaca
    10201 ccctgaccct cttcgccatg gtgctggcca tcggcatcgt ggtggacgac gccatcgtgg
    10261 tgctggagaa cgtggaacgc ctgatgagcg agcagaagct ggcgcccttc gacgcggccg
    10321 tggaggccat gcgcgaggtg tccggcgcgg tggtcgccat cgtgctggtg ctgtgcgcgg
    10381 tgttcgtgcc ggtggccttc ctgggcggca ttgcgggcaa gctctaccag cagttcgcgg
    10441 tgacggtggc ggtggcggtg gtgatctcgg gcatcgtcgc cctgaccctc acgccggccc
    10501 tgtgcgccct gctgctcaag gccagccacg aggagaagcc gctcttcaag cccttcaacc
    10561 ggctcttcga gcgcttcacc cgcagctaca ccaacaccgt cggcctgacc ctgcgccacg
    10621 gcatcatcgg caccctggtg ttcctgggca cgatcggcgc gacggccttc ctgctgcgca
    10681 tcgtgccggg cagcttcgtg cccgccgaag accagggcta cctgttcggc ttcgtcaccc
    10741 tcaccgacgg cgcctcggcc cagcgcacct tcgccgcctc ggagcagatg cgcacgcgga
    10801 tcatgtccga tgacatcgag aacatcttct tcgtgaatgg catcgacttc atcaccggca
    10861 acaaccgcgc cagcgtggcc accaccttca tcatcttcaa gccctgggag cagcgcagcg
    10921 ccaccgccga cgacatggcc aagaagttca tgggtgtagg catgagcctg tccgacggcc
    10981 tcgggctggt gttcaacccg ccgccgatcc agggcctggg caccgccggc ggcttcgagg
    11041 tgtacctgca gaaccgcgcc gacggcgacg cccgccgcct cgcccaggtc tccggccagt
    11101 tcctcgaagc cctcaggaag cgtcccgagc tgaccggcct gaacagcttc ttccgggtga
    11161 cggcgccgca gctctacgtg gaggtggatg agcccaaggc cctgtccatg ggcatctccc
    11221 tcgacagcat ctacgccacc ctgcaggcca ccaccggcac gctgtacgtg aatgacttca
    11281 accgcgccgg caagacttac aaggtcgagc tgcaggccga ggcagcctac cggatgaacc
    11341 cggaagacct gctcaagcct tacgtgcgca cggcgtccgg tgcgatggtg cccctctctg
    11401 ccgtcgccaa ggtgaagacc ctcaccggcg ccgagatggt ggagcgcttc aacggcttcg
    11461 tctccgccaa ggtgctgggc aacgccgcgc cgggcttcag ctccggccag gccatccagg
    11521 ccgtggaaga agtggccagg gaagccctgc ccgaaggctt ccaggtggaa tgggtgggcc
    11581 aggccttcca ggagaagcgc accggctcgg cctccatcgt ggccttcacc ttcggcatca
    11641 tcatggtgtt cctgatcctg gcggcccagt tcgagaagtg gtccctgccc ctggcggtga
    11701 tcatggcggt gcccttcgcc atgttcggcg ccctcagcgc ggtgctgatg cgcagcatgc
    11761 ccaacgacat ctacttccag atcggtctgg tggtgctggt gggtctggcg gccaagaacg
    11821 cgatcctgat cgtggagttc gccgcccaga aactggacga gggcatgggc gtggtggatg
    11881 cggcggtgga ggcggcgcgc ctgcgcttcc gccccatcgt gatgacgtcc ctcgccttcg
    11941 tgctcggcgt actgcccctg gccctgtcca ccggcgccgg tgccgcggcg cggcgttcca
    12001 tgggcaccgg cgtggtgggc ggcatgctcg ccgccacctt catcgccacc atcttcgtcc
    12061 ccctgttctt caagtggctg tcccgcaagg gcaagggcag cggtcgctcg accccgcccg
    12121 ccactgccgc caccgaggag gtgaagtcat gagtcccgcc cgtccgaccc cggccctcct
    12181 ggccgtgctc ctcgccgccg ccctgggcgg ctgcgcggtg ggccccgact acgtgcgccc
    12241 cgagtccgcg acgaacctgc cggcccagtt cgcgccgggc agcgccaagc tcgcctacag
    12301 cccgagcgtc gccagcacct ggtggaccct cttcggcgac cgccagctcg acgccctggt
    12361 gggcaaggcc ctggccaaca gtccggacgc cctcaccgcc gccgcgcggg tggaagaagc
    12421 cgacgcggtg atccgccagg tggacgcggc cctgctgccc caggtggacg ccaccgccgc
    12481 cggcacccac agccgcatca gccagaccac cgccacgccg ctgccgtcca gcgtcgccgc
    12541 ggtgcgcaac aacgcccgcc tggccctgac cacgtccttc gaactggacg tctggggcaa
    12601 gctgcggcgc gcctccgagg cggcccgcgc ccaggcgctg ggcagccgct acggccgcga
    12661 cacggtggcc ctgtccatcg ccgggctggt cacccagggc tatctgaacc tgcgcgccct
    12721 cgacgcccag cgcctcgtca ccgccgaatc ggtggccagc gccgagcgca gcctcaagct
    12781 cacccaggtg cgcttcgacg ggggcatcgc gtcgcagctg gacgtgcagc aggccgactc
    12841 cgccctcgcc accctgcagg cggcgctgct ccagctcgat caggcccgcg aacaggccga
    12901 aaacctgctc ggctacctgg tgggcgaacc aggtctgaag atcgctgccg gcgatctgcg
    12961 cgacctgccg atcccgcccg tgccgcccgc cggcctgccg tccagcctgc tggaagcccg
    13021 ccccgacgtg cgccaggcgg agaccgacct gatcgccgcc aatgccaaga tcggcgtcgc
    13081 caaggccgcc ctcttccccg gcatcaccct gaccggcaac ttcggccgcg agagcaagga
    13141 cctgtcccag ctgttcacgt cgccgtcgtc ggtctgggcg ctgggcgccg gcatcaccca
    13201 gccgatcttc gaaggcggcc gcctgcgcgc ccaggtggat caggtcacgg cccagcagaa
    13261 gcaggtcgcc gaggcctatc gcaaggcggt atacaccgcc ttcaaggaga ccaacgacgc
    13321 cctggcgagc ctgcgcgtgg gtgccgatgc ggaagacacc ctgacccgtg ccctgaacgc
    13381 ggccaagcgg aacctgatgc tggcccaggc ccgctacgac ggtggctact cgccgtatct
    13441 ggaagtgctg accgcccagc gcagcgccaa cgacgccgcg ctggcgtgga tccagaaccg
    13501 ccaggcccgc ctggcagcgt ccgtggatct gttccgcgcc ctcggcggcg gctggcagga
    13561 cagccagaaa ctcgcagcga acccctgacc cgccgtccgg ggcggttccg gtcgatgcag
    13621 cgggcgcctt cgggcgcccg ttgtgcttgc gccctcannn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnncgc gcctcccact cgtccaccgg catcacggtg acgcgcacct
    13801 cgggcgcctg ggcctggccg ccgagaatca ccccgcgcag gggcgacacg tcctggaagt
    13861 cccgccccca gccgagggtg acatggcgca ggtcgggctg gatgccgttg gtggggtcgt
    13921 attccaccca gccgttctcg ggacaccaca ccgccagcca ggcgtgggag gcgtcggcac
    13981 cgaccaggcg gggcttgccc gggggcggat cggtgagcag gtagccggac atgtagcgcg
    14041 ccggcagccc gaccgagcgc agggcggcga tcatcaggtg ggcgaagtcc tggcacacgc
    14101 cgcggcggcg ctccagcacc tcgaacaccg gggtgctcac ttcggtggcc ttggcgtcga
    14161 aggtgaaggt cttgtagatg atctccatca gcgcctgcac accggccagc agcggcgttt
    14221 ccggcttgaa ggcaagggcc gcaaaggcgg ccaggcgggc atcccgtggg gcgcgcggcg
    14281 agctgtagag atagcgctgg gcatcgaggg agtccgcatc ccgcgcaccg gagtggtaat
    14341 ggaagatctc ccgcacggat tcccaggacg gcgactcctc ggccttgggc agcgtgcggc
    14401 ccagcaccgt gacgcggctc tcggcccgca ccagcagacc gtcgtggggc gtgtcgaggg
    14461 ccatccaggc caccggattg ccgaagacgt cctcaccctg ctggatgcgg gcgagatccg
    14521 gatcgacggt gagctcggcc ttctccacgg tctgccacgg cagggcgcgg ggcatcaggt
    14581 ggagcaggtg ctgggcgagg gacacgggca cgccacaggg cgtgtattcg gtctcgtgga
    14641 ggacgatgta gcgggcgctg cgagtcatga tcaggcggcg aaggtggcct ggctgccgcg
    14701 gccgacgtgg ctgaagaagc gcatggcaag gcggtcggac agttcgcggc cggcatgcac
    14761 cagcgcgtcg agggcgtggg ccagggccag gctggccgct gcccgctcgg cgtctgggcg
    14821 gacgcgctcg aggctctccc aggagaaatg ctcgatgacg tacagggcct cctccagccc
    14881 ggcgatgccg atgccgtcgc cgtcctgctc gtacttgccc aggtagttga tgatcatgcg
    14941 cacctggaag gccagcgcgt gggggttggc gtcgtccatc accagcaggt ggatggccgg
    15001 cagcagctcc ggctgggcgc gatagcgggt gcggtaggtg atgatggagt cgcacagctc
    15061 cagcagcgcc tccacggcca tctcgcgctg cagcggctgg gcggtgtcgg cgtgacagcg
    15121 caggaagtgg gcaagggtgc tgcccaggct gtcgagacgt tcgatgcggc gcccgaggat
    15181 gtgcagacac cagcccatgt cgcgggtcat gttgtccatc gcgaagccgg acagggacac
    15241 gaaggtggtg agggcttcgt cgagaaattc cagcagcgcc gaggtgtcgc tgacccggcc
    15301 gcgacaatcc tcgatctcgg cggacaggcg cttgagggcg tgccagtggt cgagggagag
    15361 gtgttcccgc gcctgggtgg cgcaccaggt gaggcgttgc aggttggcgg cgaggccgtt
    15421 gccggcgcgt cggtcgagca ccgcgcggaa cagggcgagc tcggccttgc cgagggaggt
    15481 gcggaccacg tcccgctcca ccagttcggc accggcggca agctccagca gcatggcgag
    15541 ggcggcggaa cgctcggtac gccgcccttc ggacaggcgc gccagggtca ggcgggcgag
    15601 gcgggccacg gcgtcgcagc gctcgctgta gcgcccgaac cagaacaggt tctccgccag
    15661 ccgcgacgac agcgtgccgg tgaccttgac gagatcggcc acccgcaggt tcttgggcag
    15721 catgctgaag gtggacacgg ggccatcgga cagcacccag gtgtccttgc tgctgccgcc
    15781 cttctgcatg gtgagcaggc gggcgttctc catgccggcc acccgcgtca ggccgccggg
    15841 cagcaccgtg tagccttccg gcgacgcggc cgcgtagacc cgcaggccca tggagcggtt
    15901 gatgaggcga cgccgatggc cgcctgccca ggccggtgcg ccggacagct ggaccagctc
    15961 ctgggcgacc caggccgacg gattggcggc gatgcgcacg cgcagggcct cgcgctggtc
    16021 ctcgtcaagc tggtcgccga agaccacctc gctcaggccc ccggggaagg tgggcttgat
    16081 gaccagggag tcgaggtggc cgagcacatg gtccagggcc ggatcctcac cgcaccacca
    16141 ggtggcgacc gccggcatgg ccagctcttc cttgagcagg tggcggcaga tgccgggcag
    16201 gaaaccgggc agcgcggcgg attcgagcag gccggagccg agggcattgg ccaccaggac
    16261 acgcccggca cgcacggcgc cgagcaggcc gggcaccccg agggccgagt cggcgcgcag
    16321 ttcgaccgga tcgcagaaat cgtcgtcgag gcggcgcagg atggcgtgga cgcgcttcag
    16381 gccggagacg gtcttgaggt aaacggtgtc ctcacgcacg gtgaggtcct gcccttccac
    16441 cagcgggaag cccaggtagc gggccaggta gacgtgttcg aaataggttt cgttaaaggg
    16501 ccccggcgtg aggaggacgg tgaggggcac ctggttgccg tcgcacggcg cgtgggccgc
    16561 gagactgcgc tgcaggctgc ggaagaaggc ggcaagggtc tgtacctgga ggtcgcggaa
    16621 cagatccgga aaggcgcggg ataccagcag gcggttctcg agagcatagc cggcgccgga
    16681 aggggattgg gtgcgatcgg cgatgaccca ccagcgccca tccggcgagc gggccagatc
    16741 cacggcgtac agatgcagat aggtgccgcc aggcggctgg atgccctggc agggccacag
    16801 gaaacccttg tgaccataga ccagctcggg gggcagcagg ccctcggcga gggtggtctg
    16861 ggggccatag aggtcggcca gcagggcatt gaggagggtg gcccgctggg cgacggccgt
    16921 ggagatctcg cgccattcat cggccggcag gatcagcggc aggaggtcga gggaccaggg
    16981 ccggtccgtg ccgcgcgcgt ccgcatagac gttgtaggtg acgccgttct gctggatacc
    17041 acgcgccagc tgggcggcgc gggccttcag cgtgtcggcg ctggactcgt ccacgtggtc
    17101 gaggaagtcc tgccagtggg gacgtacgcc gtccgggccg aacagctcat cgtaagcgtc
    17161 ggtgcgatac gggtaatgac tgagaatcga actgggcatg tagcaacgct aacactgttc
    17221 cccgtatccg ggaaatgaac aatttcgggg cgacccgcag acgacaggcg cgcaccccgc
    17281 cgccccgtcg tctgggaccg cccgtcgtcc ggccgcaagc ccggacgacg ggcagttcgg
    17341 atcagcgccg caggtcgagc gtgaagggga attccttctc cacggccgag gtctcgaact
    17401 tcggtgcgcc cggcgtgtgc cccatgcgga agaagcgcgc cagccggcga ctttccgcct
    17461 cgaaggcatt gatcgggaag gtctcgaagc tgcgcccgcc ggggtgcgac acgtggtact
    17521 ggcagccgcc gagggaacgg ccggtccagg tatccaccag atcgaacacc aggggtgcgt
    17581 gggacgggat ggtgggatgc aggcaggacg gcggctgcca ggcacggtag cgcacgccgg
    17641 ccacgaactc acccacggtg ccggtgggct gcagcgggat cgagcggccg ttggcggtga
    17701 ggaggtagcg gtcgtcggac atgccgctga ccttcacctg catgcgttcc accgacgaat
    17761 cgacaaaacg cacggtgccg ccgatggccc cttcctcgcc catcacgtgc cagggctcga
    17821 gggcgccccg cagttccact tcgatgccac gggcggcgaa gtcgccgatc ttggggaagc
    17881 ggaattcctg gtggggcgcg aaccactccg gcttgaagtc gaggccgtga ccctgcatgt
    17941 cctcgatgac gtcgctgaag tcctgccaca cgaagtgggg gagcaggaag cggtcgtgca
    18001 gctcggtgcc ccagcgcacg aggcgctcgg gcgcgtaggg cgtgtcccag aagcgggcca
    18061 gcaggccgcg caggaggagc tgctgggcca gggacatgcg cgcgtggggc ggcatctcga
    18121 aggcgcgcat ctccaccagc cccaggcggc cggcggggcc gtcgggcgag tagagcttgt
    18181 cgatgcagaa ctcggcccgg tgggtgttgc cggtgacgtc gatgagcagg ttgcgcagca
    18241 tccggtcgat gagccagggc gggacgctgt cgccgacctt gggaaactgg cggaaggcgg
    18301 tctcgatttc gtagacggaa tcgttgcgtg cctcgtcgat ccgcggggcc tggctggtcg
    18361 gtccgatgaa caggcccgag aacaggtagg acaggctggg gtggttgtgc cagtaggaga
    18421 tcaggctgcg cagcaggtca ggccgccgca ggaagggcga gtcggacggc gtggcgccgc
    18481 ccagcacgaa gtggttgccg ccgccggtgc cggtgtggcg accatccacc atgaacttct
    18541 cggtggtcag acgggagaag tgggccgcgt cgtagagatg ggtggtgcgg tccaccagct
    18601 cttcccagga gccggccggg tggatgttca cctcgatcac gccggggtcg ggcgtgatgc
    18661 ggaagtgggt caggcgcgga tcggtgggcg gctcgtagcc ttccaggacc accggcgtgc
    18721 cgaaggcctc ggccgagctc tccactgcgg cgacgagttc cagatagttc tccaggcgcg
    18781 acaggggcgg catgaagatg tgcagggtgc cgttgcgcgg ctccacgcac atggcggtgc
    18841 gcacgatcca gtcggcagac tcgaaggact gcggcgtgcg gtcggtgggg ctgagcttct
    18901 cggcggcccg ggcggccttg agggcggcgg cacgggcggc agcgccgctg ccgaggccgt
    18961 aggtctcgtc gtccttgaag gcattgaggg cgttgagcac cgccgtctgg ccgccgggca
    19021 gctgacggcg gatggcctgg tattcaggca gcggcgggaa gctctggtgg gcgtccggcg
    19081 gattgacgta ggggtagctg gtggccgtca cccagggctg ggaatccagc ggcagacggt
    19141 agccgatcgg ggaatcgccg gggatcaggt agcagcgctc gtcccgcagg aaccacggcc
    19201 cggtctgcca ggcgccggtg taggtgttga tggagatggg cagcacgtag cccacggcgt
    19261 tggcaaggcc gtagtcgaag acgcggcgca ggcggtcgcg ctccagcgga tcgtccaggc
    19321 gggcgtcgaa gggatccacg ttgcccggca ggcggcgctc gcgccacagg tagtagaagg
    19381 catcctcgta gccggcgaag acgcggcgtg cgtcgaggcc gaggcgctcc gccacgtccc
    19441 gcaggaaggc gccggccagg tcggcgtcgg cgtcacccgg cgtggcggaa tcggcgatga
    19501 ggtccggatg ggtccagatc ggctcgccgt ccttgcgcca gtagcagttg agggaccagc
    19561 gcgggagctg ctctccggga taccacttgc cctggccgaa gtgggccagg cccgtggggc
    19621 cgtacttgtc gcgcagacgg tggtagaggt cttcggcgcg gacgcgcttg gtggggccca
    19681 tggcctcggt gttccattcg gcgccgtccg ggtcgtcgat ggacacgaag gtgggctcgc
    19741 cccccatggt gaggcgcatg tcgtgggcct tgaggtcttc gtcaatgaca tgacccaggg
    19801 cttcgatctc ggcccattgt tcctcggtat agggcttggt gacgcgcggc gcttcgagca
    19861 cgcgggtgac gctcatgtgg tgggcgaact cgcactcgca ttcgtccacc agaccggtga
    19921 tcggcgccgc ggacgtggga tcgggcgagg cggccagcgg aatgtggcct tcgccggcga
    19981 agaggccgga ggtgggatcg agacccaccc agccggcgcc ggggagatac acctcggtcc
    20041 atgcgtgcag gtcggtgaag tccacctcgg tgccggacgg gccgtcgagg gccttcacgt
    20101 cggccttcag ctggatcagg tagccggaca cgaagcgcgc ggcgaaaccc aggtgacgca
    20161 acagctggac cagcagccag gcggagtcgc ggcacgagcc ggtgcgcttt tcgagggttt
    20221 cttccggtgc ctgcaccccg ggctccatgc ggatcacgta gccgatgtgc tgctggagct
    20281 tggcgttgat atccaccagg aaatccacca cgcgacggct gtcccgcggc acgctgtcca
    20341 ggtaggcctg gaacaggggc gtgggcgcgt ccttgaggcg gaagggcgcc agctcatggc
    20401 tcagattgcc ggcgtactcg aagggatagt tctcggcttc gggctcgagg aagaaatcga
    20461 aggggttgat gaccgacatc tcggccacca gatccacctc cacggtgaat tcggtgactt
    20521 tttccggaaa gaccagacgg gccagatagt tggactgggg atcctgctgc cagttgatga
    20581 agtggggctt gggcagcacc ttcagcgagt aggcaaggat gcgggtgcgg ctgtggggcg
    20641 ccgggcgcag acggacgacc tggggtccga gggtgacggg gcggtcgtat ttataggagg
    20701 taaggtgatt gagggcgacg tgaatggtca cgggatactc cgatctctgg ttcgcggggt
    20761 gaaggcgcgc ggggcttgcc ggcagtttta gcaagcttca cgccagaaga tttacccgca
    20821 gcaggttaag gttttaccgg cgatacaggg gcttgcagag ggtgcactag gcagggcggg
    20881 caatttcgag cctcgccttg gggcacgcac cagaatggac aaggccggac gcaccgcgct
    20941 ggtgcgcccg gctggatgcg acggatgggg ggaaacaaga tcctttgcct cagcggcgtc
    21001 cgcccaggat ggagcccagc acaccccgga cgatctcccg gccgacctgg gaaccgatgg
    21061 tgcggacgac gctgcgggcg gcggtttcgg cgaggccggg gtgccggccg ccgcgagggc
    21121 cggtggtgcc gaagaggatg tcaccgagcc caccgagcag accgccccca tcctgggccg
    21181 gtgccggcgc ggacgccccg ccgggggctc cggccggtgc cgcctgggcc gccggagcgc
    21241 cacgcaattt ctcgaacgcc gactcgcgat ccacgatctg ctcgtagtgg ccgaagatca
    21301 ctgaattttc gatcgcggcc ttgcgttcct cgccgctgag gggccccagg cgcgacgcgg
    21361 gcgggacgat gaagccccgc tcgacgatgg acggccggcc cttctcgtcg agaaaggata
    21421 ccagcgcctc gccgacgccc agttccatga tcaccgtggc ggcgtcgaac tcggggttgg
    21481 cgcgcagggt ctcggcggcg gtcttgacgg ccttctggtc cttcgggctg aaggcccgca
    21541 gggcgtgctg gacccggttg ccgagctggc cgaggacgtt ttccgggatg tccagcggac
    21601 tctgggtgac gaaatagacg ccgacgccct tggagcggat caggcgcacc acctgctcga
    21661 tcttctccag cagggccttg ggcgcctcgt tgaagagcag gtgggcctca tcgaagaaga
    21721 acaccagctt gggtttgtcc agatcgccca cttccggcag atgctcgaag agctcggcca
    21781 gcagccacag caggaaggtg gagtagagct tgggcgagtt gtagagcgcg tcggcggcga
    21841 ggatgttgac gacaccgcgg ccgtcgccgt cggtctggat caggtcggcg atgtcgagca
    21901 tcggctcgcc gaagaacttg tcgccgccct gcgcctcgat ggtcatcagg ccgcgctgga
    21961 tggcgccgat ggaggctgcg gagatgttgc cgtactgggt agtgaagtcc ttggcgttgt
    22021 cgcccacgta ctgcagggtg gcgcgtagat ccttcaggtc gatgatcagc aggccctggt
    22081 cgtcggcaat cttgaaaacc agttgcagca cgccggcctg ggtgtcgttg agattgagca
    22141 ggcgcgccag cagcagcggc cccatgtcag agacggtggc gcgcaccggg tgacccgagg
    22201 tgccgaagac gtcccagaac accgcggtat tggcgcgcgg cgtgaaatcg tcgatgccca
    22261 gggcggtgag gcgctccttc agcttcggtg acacgttgct tgctgcaccg atgcccgaca
    22321 gatcgccctt cacgtcggcc atgaagaccg gcacgccgat ggccgcgaag gactcggcca
    22381 tcttctgcag ggtgacggtc ttgcccgtgc cggtggcgcc ggtgatcaga ccgtgacgat
    22441 tggcaagggc cggcaggagg acgatgtcct tggggccgga cttggcgatg aggagcggct
    22501 cggtcatggg gatctccagg cgggcgaaag ggggcggact gcgccaatcc agcaattatc
    22561 ggtcaccccg cgggatttta gggagcgcct ctgcgtgccg cggcacggaa tgaccgcgga
    22621 accggcatcg ccgggccctg cgggaggctg cgcgaagggc tgccggcgcg gtaaaatcct
    22681 gcctttcggg tgcactttca aggacacaga atcatggcgg gtcacagcaa atgggccaac
    22741 atccagcatc gcaagggtcg gcaggatgaa aagcgcggcc gggtcttctc ccggctggcc
    22801 aaggaaatca ccgtcgcggc ccgcatgggc ggcggcgacc cgggcttcaa cccgcgcctg
    22861 cgcctcgcgg tggaccgggc caaggccgtc aacatgcccg ccgacaacat cgaccgggcc
    22921 gtcaagaagg gcaccggcga gctggaaggt gtcacctacg aggaggcccg ctacgaaggc
    22981 tacggtatcg gcggcgccgc ggtgatggtc gattgcctca ccgacaacaa gacccgcacc
    23041 gtggccgacg tgcgtcacgc cttcaacaag ttcggcggca acatgggcac cgacggctgc
    23101 gtggcttttc agttcaagca ctgtggccag ctgctgttcg cgccgggtac ctcggaggat
    23161 gcgctgatgg aagcggccct cgaggcgggc gccgacgatg tggtgagcaa tgaggacggc
    23221 tccatcgagg tgctcaccga gccctaccag ttcggcgagg tcaaggacgc tctggaaaag
    23281 gccggcttca cgccggaatt cggcgaagtg acgatgaagc ccctcaacga ggccgaactc
    23341 accggcgatg acgcggtacg catgcagaaa ctgctcgacg cgctggaatc cctcgacgac
    23401 gtgcaggcag tctacacctc tgccgtcatg gacgacgcct gatcgcacgc gcgccgttgc
    23461 cgccccgatg tgatggacgg ggtggttcgg cggcgcggga gcgggctccg ctgcaaggcg
    23521 ccgcgcgcct cagcccaggg cgaaggcgac cgcgccgctc atgttgtcgg caaccgagaa
    23581 cccgcagccg atatagcctt tttcgccaag gatgaagtcg tcgcgcttga tgcagtgctc
    23641 ggctgcgcct tcttccagca ggtaggtccc gttggtggat gaatcggtca gatagaacag
    23701 accattgcgc agttcgatac gcgcatgctg gcgggacaca cgcggatcca cgacgacgat
    23761 gtcgttaccg gtctcacggc cgaagagcag ggtcggggaa ctcgcgtcga ccgtccagct
    23821 gcgcccctgg aagctgacct tcatctgggc cttgggccgc acgctggcgt ggcgcagttc
    23881 cacgggactg ggcggcgtgg cctcgcccag ttcgagcacc tggaagggca gctggtcgtt
    23941 cgcctggacc atggacgaga ggagcttgcg catggccggc gacagggctt cactgaaggc
    24001 ctcggagacg gaaacggtgt ccgggttgtg cccgacggcc agccgcgagg ccagggtttc
    24061 ggcccccttc agatcgtccg gcgcctcgga ttcgagcacc aggcccgcgc gcagaacgag
    24121 cttgatcccg ctcatcggtg gcagggcacg gacccgttcg cgcatctcgc gggcggcgta
    24181 gagcgccgcg tcggcatccg caaagcgcgc caccagcacg tccggacgca cctcgatgcc
    24241 ggacgtggca taggcttcag cgcagcggga catgcggttc aggcagcgtt cgacagcgta
    24301 ccctgcctcc gcatcgccga gcttggcggc cagccggaca ccgccggcca catcggcaaa
    24361 caggatgcac ggttgatgtc cggccccgcc ggcgcccgtc acgacgcggg acggacgggc
    24421 atggacggat tcacgggacg acgaattctc agcaaaggcg ggggcgtggg tcatggtgac
    24481 ttctcacgga gaaaccccag actgaccgca gcctccgcgg gcgggacgga gggcagttcc
    24541 ggggcatcga atacaagatc tccctgatct accttggtat cgcacgagac attcaggaag
    24601 tcaaacagct ttccgtccgc caggtgggac gggacaacgt tctgcaaggc cgtggcgatg
    24661 gacacgatgc gcccgggaaa acgcgcatcc cagtcccgca gcatggcctt gatctgcttg
    24721 cgctgggcgt tctcctggga gccgcacagg ttgcacggga tgatgggata gcccatcccg
    24781 cgggcgaagc gctcgatgtc tttttccggg cagtaggcca gcgggcggat caccacgttc
    24841 tggccatcgt cgctcaccag cttgggcggc atggccttca tcttgccgcc gaagaacatg
    24901 ttcaggaaca gggtctcgat catgtcgtcc cgatggtggc cgagggcgat cttggtggcg
    24961 ccgagttcct tggcggtgcg gtagatgatg ccgcggcgca ggcgcgagca cagggagcag
    25021 gtggtcttgc cctcggggat cttgtccttg acgatcttgt aggtgttctc ggtgacgatg
    25081 cggtattcga cgccgatgga ctcgaagtag gcgggcagga cgtggtccgg gaagcccggc
    25141 tgcttctggt ccaggttcat cgccacgatg cggaaattca cgggcgcccg cttctgcagg
    25201 gccagcagga tagacagcag cgagaaggag tccttgccgc ccgagacgca gaccatgacc
    25261 acgtcaccgt cccggatcat cccgtagtcg gcgatcgcct cgcccacctt gcgttccaga
    25321 cgccgcttga ggttgcggaa ggtgttggac gtgcgggatt ccggcgtggg agtgtcgatc
    25381 ggaaggggga gggctgcgcc cacggcgaga tccatgatgt cgtgatgtcc gattatagta
    25441 tggggatatt cgggccttcc ccgaacgcaa tgtaatttaa cttagaggtg cgccgagcgc
    25501 cccccatcca cggcaatgat ctgccctgtg atgtaaggcg cattgcacag caaaaaaacc
    25561 accgtgcgtg ccacgtcgtc gggctcaccg acgcgcccga ggagcgtgtg gcggacgacc
    25621 gcatcctgcg ctgcgggcgg gaactggcca tcgtcgggcc attcgatggc gcccggcgcc
    25681 accccgttca cccgcactgc cggccccagc tccacggcaa gcgcgcgggt caggccgagc
    25741 agcccggcct tggcggcgca gtacagtgca tagccatgca ggggccgttc tgcgtgcacg
    25801 tcaacgatat tcacgatgct tccgcgggtc gccagcagat ggggcgcagc cgcctgggac
    25861 aggaacaggg gcgccttcag attggagccg accagatcgg tccaggcggc ttcgtccata
    25921 cgccccatgg gggtcgggaa gaaactcgat gcgttgttca ccaggccgtc gaggcggccg
    25981 aaacgggcca ccaccgcgga caccagggcg ggcagcacgg ccgtgtcgag aagatcggcc
    26041 tgcagcaccg ccacggaccc gggccgccgg gcgttgagct cggccgccag cgcctcggcc
    26101 tcgtcgcggg agtgccggca gtgcacggcc acccgcgcgc cttccccatg aaggcgacgg
    26161 acgatggcgg cgccgacgcg ccgggctccg ccggtgacga gcatcaccgg cgcgggggag
    26221 gctgaggaca tgactgacgg aaggaggcag cggggatgaa cccgttatct tacgggccgg
    26281 acgcgacgcc cgggcgattt tctcggcccg gcggcccgac cgggcggcac gctaaaataa
    26341 cggttttgtg ccccgactgt tgacatgaac ctgcccccgc cttccccgga tgccctcgcc
    26401 cagagcgagc gcctcgttgc ccgcctgcgt gacctcatcg cccgcgaagg gggctggatc
    26461 tccttcgcca cctacatggg cgaagccctc tacgcaccgg ggctgggcta ttacagcggc
    26521 ggctcgcgca agttcggtcc gggcggcgat ttcatcaccg caccggaact cacgcccctc
    26581 ttcggccagg ccgtcgccgg gcagctcgtg gagatcatgg cccagtcggc gccacagctc
    26641 atcgaggccg gcgccggcac cggcctgctg gcggccgacc tgctcctcga actggaacat
    26701 cgcggtgccc tgccggagcg ctatgcgatc ctcgaagtgt ccggcgaact gcgttcgcgc
    26761 cagttcgaca ccctggccga aaaagtgccc catctcgcct cgcgggtgtg ttggctcgac
    26821 accctgccgg agcacttttc cggtgcgttg atcgccaatg aggtgctcga cgtgatgccc
    26881 gtgcatctgg tggcgtggcg acccgagggg ctcttcgagc ggggcgtggc gatggatgac
    26941 gcaggccgct tcgtgtgggc cgacgtgccc gccagcggcc gcgtggccga agccgccgct
    27001 gcgctggagc gaccgttgcc gtccaccggc gagtacgtga gtgagctgaa ccttgccagc
    27061 cgggcgtgga tcggcgaatg ggccgcgcgc ctcgacaagg gcgccctgct cctggtggat
    27121 tacggctacc cgcgcgccga gtactacctg gacagccgca gcaccggcac cctgctgtgc
    27181 tactaccgtc accgcgccca ccccgatccc ttcctgtggc cggggctcaa cgacatcacg
    27241 gccttcgtgg atttcaccgc gatggcggag gcgggcttcg acgcaggcct cgacgtgctg
    27301 ggctacacca gccaggcgac cctgctgctc aactgcggcg tgctggaatg tctggcgcgg
    27361 cgtgcgccgg aggacagccc ggactacatc cgggcgtcac gggcggtgca aaggctcacc
    27421 ggcccccacg agatggggga actgttcaag gtcattgccc tggggcgcgg catcgacgga
    27481 ccactcctcg gctttcgccg cggcgaccgc ctgcacacac tctgacccac ggaggcggca
    27541 cgccggggca atggcggccc cggcctgcgt gccgtgctca ttcctggaag cggtagatcg
    27601 cgctcaggaa ggcgtccacg tcggtgggca cgaaatgccg ctcggcgtgg gtgaccgcca
    27661 cttcctgcag acccagggcc agctcgggtg gcaggaggtt ggcgttgagg atctcatccc
    27721 cgtattccgg cgcctgctcg tccaccggat ccatgcgcgc ctgggcgccg ctttccatgt
    27781 ccatgtagat caccatttca gcctccgtcg gggaagcgca cgatgcctcc cacatagttg
    27841 ctaacggctg tccttcagaa aggttgaagg ccgccccgtg agacaccgtg cacgaaccgt
    27901 gccggtgtga cgcgacaagc cggtgatgtc agcatggacg aggtcgccac gccgcgccag
    27961 gacacctgtc acggccgacg cccgatgccg cttacagccc gtgggcggcc agccaggcgg
    28021 cgatcggatc ggcggcgatg cgccagcccg cctccagcat catgccgtgg cccaggcgcg
    28081 gcacgatgta ggcctcggtg ccataacggc gggcggtcat gcgcacctgg tcgggcggga
    28141 tgagcatgtc gtgttcggct cccaacacca gcatgggcgg caatgcgaca tggccgaggg
    28201 cgggcaggtc gaacagggac atatcccaga tcgcccggtg ggattccggc tggcacaggt
    28261 ggtaatactg gcgcaggcgc accagctcga ccggttcgtg gaagagggcg tcccgcaggg
    28321 tgtccagatc cggctggccc ccgcccatga tgcggttcag gtcgcccatc atgttcggtc
    28381 gcgaaaaagc cagcccgaag gccgagccca gcaacccctg gggcggcacc gcggccatca
    28441 gcaccgccgc cggcacgcgc tgccgctcca gacacttctg caccaccatg ccccccatgg
    28501 aatggccgat cagcaccggc ggcgcgggca ggcggtcggc cacgtcggcc acgtcagcca
    28561 cgtaatcggc gagggaatag ctgtccagaa aggcccggtt ccggctgctc ccgtgccccg
    28621 acagggagac ggcgtacgcc gcatagccca gactcgcgaa atggggcagg aagtgctcct
    28681 cccagcacca cgccgccgtg aaggcaccat ggacaaagag cagcggcgtg ggacggggcg
    28741 tgccggacgg caggcgacac aggacttcca ggtcaccgaa acgggcggat ttcatcgggg
    28801 ctgttcttgt tgtggtgtcg tccgcacgga tggcggttgc cgagaccgga cgacgcaagt
    28861 gtggggcgtg gggcgggaac ataccggaat cctggggtgg tgagaagcgg tccaccgcgg
    28921 gcacgggcaa tcggggagtg cgctgcacgc acgctcgaag cgtcctgcag cgcatggtcg
    28981 gaccgagtgt gccggaagag ccggcccgac gcgcgtagaa agaggacgtc cctggagatc
    29041 ccggtacgcc ccctcgattc cggcgctatt tgaattcgaa acgaacgtcc ttgaccacgt
    29101 gatgcttgcc ccaatggaaa ttgcagacac gggcaacttc cagcccctcc ttgctttcgg
    29161 ggcgacatcc accgctgatc ttgacgattt tctccccctg gagcgagagg ggaaactcga
    29221 ccaccagccc ctcgggaatc tcgtgttcca gttcgacctt ttccgcaaaa tcatcgacga
    29281 gcgcaaagat cacctgccga tcggctttca acacgtagag gtcgtgctgg agcgcggcgc
    29341 cgggcacgag acacaattcc agcgtcgatt tctcgacgat ctgctgcctg cattggggtg
    29401 gatccgcagc cagcgcaccg gacgcacaca gtcccagcaa tagcccttgc acccaaggct
    29461 tcatcgtgtc ttccatcgtt gactccggca tggcgaaagg cgccgtgcat gcctcaggat
    29521 gcccgatccc gcagcgaaat tatacgtcat gagcgcgtcc atgctgggcg cccgccgacg
    29581 cattccgctc tcgaaagtgc ttccccgctc aggccggcta cggttcaata ccgccttcgt
    29641 tctgcccgtc ccccgcccca tgctcaaatg gctgctcgcg atcgtcgtga tcgccgtcgt
    29701 ctccggtctc ggccgcggca tttcgccggg gcgctggcgc atcggccacc tgccggggga
    29761 tctgcatttc catctgtggg ggcgccacat ccacatcccc ttcaccagca ccctgctgct
    29821 gtccctggcg gcgatgctgg tgatccggct gctttagggc aggggcgcgc ggacagcgaa
    29881 ggtgaccgtg tcgaggacgc ggtcgtcggc agcgaggagt tcgagggtgt gcaggcccgc
    29941 caccggcgtc cacgcgaggt gggcgccgcg accgatgcgc tccccgtcga ggcgccagga
    30001 caggccgggc agcgccgggc tcgcctcgaa gcggatgcgg aaggcgggat catcggcgag
    30061 ggccatccca tccaccaggc tgccggcagc gggatggagg atgtgcgcgg ccgggcgggg
    30121 catgccgacc ttgtccgtct cggtcccgcg gatgaaccat tcccggcgcg ccggctcgac
    30181 cggcggatcg aagaccacca ccgacgacac cacaccgacc ggcgccgcga ccgtgcgggc
    30241 cggcacggtg ccccggtaca ggccgcgcag gatctcctgc caggcggcgc ccgcggagga
    30301 ggcggtccac cgcccgcccg cggcccacag gaccagcgtg tagcgctcgg cgaagcccgc
    30361 cacctccatg tcgcgcccgt cgccactggc catgcgctgg acaaaaggct gaggcacgcc
    30421 ctccgcgtcg tcgaggacca gcatgtcgcc gacgatgaag ttcgcctcgg acctgagcag
    30481 gcgccgcggc gccgcgcgct caccctgcgt caggcgcacc tcctgccaca gaccgccgtt
    30541 cgccaggctg cggtagaagc ctgccagctg cagcaggctg aggtccggcg ccgggccgcg
    30601 ggccggatcc agctccatca cgcgcaggcg ctcgggcagc gcctcggcca ccaggcgctg
    30661 caccgccgcc gccggctcgg gcagcccgtc ggccagggca cggcgggcac tcacccacag
    30721 cttctcgtcc ggcagggtgg gcgcatgatt ggacagggcg tcctccagca aggtcgcggc
    30781 ggtcaggacg cgacgttcca gcgcaagccc atagaccagc ggcgcgagca ggctggccga
    30841 cgtgcgcggg acaggcagcg cgtcggccgc cgccggttcg agggcgccca cataggcgat
    30901 cacgtcgccg ctggcgttgt cgagcaccac cgccgccgcc tcgctgacgc cgctgcggcg
    30961 cagggcggcg aggaccagct tctgggcgtc ggcgtccagg gtgctgacga tccgctcgcc
    31021 gggctggtgc aggagctgcg ccgccgcgtg gggcgccagg tcccagcgcg gctggagctg
    31081 ctggcggtcc agggtgttct gggcgaggcg ctgggccgag gcgcaggcgg cgggcgtgcc
    31141 gaggcgctcg gcgagcatgc aggcccggtg gccgaccacc tccggccgcg cgttggggcc
    31201 ccgcagcagc acggcgagga tcagggattc cggccggttg agttcgcccg gcgtcttgcc
    31261 gaaaaaggcg cgggcggcgg catgcacgcc ctgcagatcg ccgcggaagg gcgccaggtt
    31321 gaggtaggcc tcgaggatct ggtccttggt ccagcgcgcc tccagggcct gggcggcaaa
    31381 ggcctggtcc catttctggc cgaggctgcg gcgcggctgg ccatcgcgac cgagggccag
    31441 ctcggggtcg agcagcccgg cgagctgcat ggacagggtg gaggcgccgc gcttgcggct
    31501 gttccacagg ttatgccaca gggagccggc gaaggcctgc cagtccacgc cagagtgctc
    31561 gaagaagcgc ttgtcctcgg cggccagcag ggcctccttg aggaccggcg agagggctgg
    31621 caggccgacc cattccaggc gacgcacctg cgggtcgagg cggcgctcgg ccagcggcgt
    31681 gccgtcccgc gccagaagca cggccaccga ggggcgcgcc gcggccttca cgtcggcaaa
    31741 ggccggcagg ggctgatgcg cgcgggcagg cagcgccggc agggcgagga tggcagcaag
    31801 caggacggcg ccgatccggg tttccggacc cgtcgcccgg cgcggacgca cgccggcacg
    31861 cgggccggag aggcggccgg ggcgcagacc gggcagggac gggacgcgga gcaggcgcat
    31921 gatcgggcgg cattgtaggc cgcgggccgg cgggcgcagg cgggttcagg tcacgccggc
    31981 gtcgtccggg ctgtcaattt gtgtgtcatt ggcggccccg accggcacct ccgtacctcg
    32041 gcccagggcc ttcactgcgt cgatccgcgc ggcatgaacg gcggcgggaa tgcgcgcctt
    32101 gtcggcctgc tggcgggcga tggcgccggc gtccaccgca cgcaccgcgg ccagggccgc
    32161 gaggagacgg tcccgcgcgg gatagggccg gccggcgagg cccgcacggc cggcgtggtc
    32221 gcaggcgcag gcgtcgagca gcgcgacgaa gcgctcgggc cggcgcaggc cgtcgcagcg
    32281 ctcgatgagc ttgacggtgg tgtccgggcg cagctcgagg gcgcgatgga cgatgccatg
    32341 ctcgcgggcc gtcatcaggg ccagatcgcg gcagtcgacg gggcacttca ggcgttcgca
    32401 gacggcgcgg gcgaggcgct cgctggacgc ctcgtggccg tagtggtggg gcagcacggg
    32461 ggccggcgtg acgcccttgc ccaggtcgtg gagcaggcag gcgaagcgca ccggcagggg
    32521 ctgaccggtg gcagcggcgt ggtcgaggac caggcacagg tgctcgccgg tatccacttc
    32581 cgggtgatgc tttgccggct gaggcacgcc gaacaggcga tccacctcgg gcagcaggcg
    32641 cgccagggcg ccgcagtcgc gcaggacccg gagcatccgc gaggggcggg cctccatcag
    32701 gccgcgggcg agctcctgcc acacccgctc cggcaccagg gcgtccacct cgccgtcgtc
    32761 caccatgcgg cgcatcagga caagggtctc gggcgccacg cggaaatcgg caaagcgggc
    32821 agcaaagcgg gcgaggcgca ggatgcgcac cgggtcttcg gcgaaggccg ggctgacgtg
    32881 gcggaacacc cgcgcggcga ggtcggcctg accgccatag gggtccatca gggagccggc
    32941 ttcgtcgcgg gccatggcgt tgatggtcag gtcgcggcgg gcgaggtctt cttccaggct
    33001 caccgacggg tcggtgtgga agacgaagcc ggcgtagccc ggcgcggtct tgcgctcggt
    33061 gcgcgcgagg gcgtattcct cgtggctggc cgggtgcagg aagaccggga aatccttgcc
    33121 caccggcctg aagccgcggg cgaggagatc ctccggcgtg gcgccgacca ccacccagtc
    33181 gcggtcctgg accggcagac cgaggagttc gtcgcggatc gcgccgccga cgcaatagac
    33241 cttcatggcc ggggtttccg gaaggcgtcg aagtgcccgc gccggttggc ccggccgagc
    33301 caccggggca ccacggtctc cagcgcggtg ggcgtgcggc cccagggctg gggcgggccg
    33361 ccggtgatgt tgtcggcgcg catggagcgc acgttgtcgc ggctcatcag cgggccgggc
    33421 aggcgctcca gcaccgccgc ctggagcagg gcgaggggtt ccgacagggg cacgatccgc
    33481 gtgctgcggc cggtcaggcg agccacatag gccaccagct cggccagggt atacaccgtg
    33541 gggccggcca ggtcgcaggt gaggccggcg gcgtccgggc ggaccagggc ctcggcgacc
    33601 acgtcggcca cgtcctgcac gtacaccggc tggaagcgtg caccggcgcc gcccaggggc
    33661 agcaccggga aggtccgcac caggcccgca aagaggttga ggaagctgtc gccgggcccg
    33721 aagatcaccg acgggcgcag gatcgtccac gccagttcgg gcgcgccggc gcggatggct
    33781 gcctcgcccg ccgccttgga gcgctggtat tccgacggcc cgtccggcgc ggcgccaagg
    33841 gcgctcacgt ggacgaggcg cgtcacgccg gcctggcgcg ccgcggcgac gaggagggtc
    33901 ggcagctcca cgtgggcgcg ggcgaagtcc ggaccgtagg gctgacccgg acgggaatgg
    33961 agcacgccga ccaggctcac cactgcgtcc acgccctgca tcaggcctgc gagggtgggc
    34021 ggatcgaaga cgtcggcctc gaccaggtcc acatcgggca gggtgaggag gtgccggacg
    34081 cgggcacggc gccgggtggg aatgcgcagg gacaggccgt cccgtgccag tcgcgccgcc
    34141 acggccgtgc cgacgaagcc gctgccgccg acgatgagca ccttgcggga ggtcatgggg
    34201 tcatcctcgc gtgatcctgg gattcgtcag agtgccgcag cggccaacaa ttcccggccc
    34261 ctgcgccgac cgtcattcat tctccagccc cgcgcccggc gccgggctgc ccaggcccga
    34321 ggcatcgccg ttgccgggca ggacggtgcc gaggcgggcc ttgagggacg gcgcgcgacc
    34381 gtccagcagg gcggcgtaga cgatggaatt ggccatcacc ttcttcacgt agtcgcgggt
    34441 ctcgttgaag gggatggtct cggcgtagat ggcgccttcc aggggcttgc tgtcgcgcca
    34501 cttcctggcc cggccggggc ccgcgttata ggcggcggag gcgagcaccg gatggttgtc
    34561 gaggccttcc agcaccatgc gcatgtaggt ggtgccgagc atcacgttgg tgtcgggatc
    34621 ccgcagccag ccaatgttgt agcccttcat gccgagcttg ccggcgatcc actggccggt
    34681 ggacggcatg atctgcatca gtccctgggc gcccacgccg gagcgggcgg cggtcacgaa
    34741 gcggctttcc tggcgcatca ggccgtagac ccaggcggtg tccaggtcgc ggctccgggc
    34801 gttgggttcg atgcgctccc ggtagggtgc gaggtagcgc aggctgtagt cgtgttcgct
    34861 gcgggtgcga tcggcggcgt tgatggcccg gtcgtagatg cccagctcct gggcgtaggc
    34921 ggcggcggcg aggagaaagc ggtcgtcccg gttgcgcatc gaccagttcc actcccgcgt
    34981 gccctcggtg cgcatgtcga gccggaagag ggctacggcg cgctgcacgc cgggggtggt
    35041 gcgcaccagg gtcgcctcgt cgtcgctggg gccgcgggct tttttgggca gtacaaagct
    35101 gcgcccgagc tcctcgtcgg cgaggatgct gtagaaggcg gggtggccgc cgatctgctc
    35161 gaaaagccgg cgcgccgcgt cctgtttgcc gagggcggcc tgggcacggc cgagccagta
    35221 ggcccagtcg ggctgttccc gcaggctcgc gggcatggcc tcgatggcgc tgcgcacgcc
    35281 cttccagtcg ccggcgcgca gggccgcgcg ggcccgccag gcgatctgct cgtcgctcat
    35341 gggcgtgtct ccggcggcgc gataccaggt gtgggcctcc ggcatcagac gctgggctgc
    35401 ctgccaagcg atctggccga gggcgtaacc ccgctcggcg ggggagaagc gctcgtccat
    35461 gcgctgatag cgcacatagg cgccgagggt gtcgtcgcgg gcgaggcggg ccagggcgat
    35521 gagggccagt tcgcggccgg gacgggtggc ggagaagttg ggcggcaggc ggtccaggta
    35581 gcccgccgga ttggcggcgg ccttgtccag atcggacggt gccggcacct ggtcggcggg
    35641 cagccagctg gcggcgtagc gggcatgggt gttgcgcttg gcttccatca ggcggcgcac
    35701 gcgggcccag atctcgtccg ggctgaccag agcctcgcgg accagggtct ggagcaccgg
    35761 cacacaggat tccggcgtgt ccacgaggtt gaaccacagg ggccgtgcct ccgccagcgc
    35821 catgccgtcg ccgttggcga ggcgggcctg gaactggtag cactggagat cctgctccgg
    35881 ctgctccatg aaggggtatt cgatcgcgaa ggtgccccac tcgccgcgcc gccccaggcc
    35941 gcgcagccat tcgccgcgca gcttctcggc gagccaggcg ccgcgattgc gttcgaggaa
    36001 ttcccgcagg gccgccggat cgatgtcggc cgggcgcgcc acctgggcgg agagccacca
    36061 gtattcggga tagggatcga ggacgtggcc ggccggcgcg gcgtaggccg ccagacgcgc
    36121 ccggtcgccg ctgcgggccg cgtcccgcgc ggccaggata cgttcgtcgc ccgtatcggc
    36181 ctgcgccgcc aggaccgccc acaacactgc cgcgatggcg gcccgcacgc tattcttcat
    36241 cgactgccag attgacctga aatgcccgcg gagcatagca cagtgaccca gcccccctcc
    36301 ccgcccccgg cgccagacgg caccgccgag cgccgccgcg ccctgcgcag cgcccgcatc
    36361 gccgc
//
LOCUS       DAOQMM010000025        35797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_302_length_35797_cov_45.107465, whole genome shotgun sequence.
ACCESSION   DAOQMM010000025 DAOQMM010000000
VERSION     DAOQMM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 35797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35797
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_302_length_35797_cov_45.107465"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /gene="ettA"
                     /locus_tag="PLH85_06315"
     CDS             complement(<1..1303)
                     /gene="ettA"
                     /locus_tag="PLH85_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNB64209.1"
                     /translation="MAQYVMSMLRVSKIVPPKRQIIKDISLSFFPGAKIGLLGLNGAG
                     KSTVLKIMAGVEKEFDGEVQWQPNMSIGYLPQEPQLDAAKTVREEVESGLGEVMEAKQ
                     KLEEVYAAYAEPDADFDKLAELQAKYENIIATSGADVETQLEIAADALRLPPWDARIG
                     PLSGGEKRRVALCKLLLSRPDMLLLDEPTNHLDAESVEWLEQFLCRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEQRLEQEAKQEGARMKAMKQELEWVR
                     SNPKARQAKSKARLARYEEMSSVEYQKRNETQEIFIPVGERLGDKVIEFTDVSKGFGD
                     KLLMDKVNFSIPPGAIVGIIGPNGAGKSTLFKMVTGQDTPDSGSVVIGPTVKIAYVDQ
                     SRDCLDGSKTVFDELAGGADILTIGKFEMPSRAYIGRFNFKG"
     gene            complement(1388..2101)
                     /locus_tag="PLH85_06320"
     CDS             complement(1388..2101)
                     /locus_tag="PLH85_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain N-acyl amino acid synthase"
                     /protein_id="HNB64210.1"
                     /translation="MTDRACHSHYHSVEPPPRGVHLQDPRRFTLERADPVRHGQTVRM
                     LIDRMYSWRGLTPVGSHHGPHHNHHRTTLVTSSADEVFGTITVGIDSPAGLQADPLYR
                     HELDAFRQRGRLLCEITGLAVDSPVQAKEALGGLFHLAYIQARLIDKADDVVIEVNPR
                     HAPFYQRLLGFGQIGPLRHCQRVNAPAVLLHLAISHMDDHIARFGGTSPKHTRTLYPY
                     FFSLAEQAVLLAQMQQAAN"
     gene            complement(2280..4004)
                     /locus_tag="PLH85_06325"
     CDS             complement(2280..4004)
                     /locus_tag="PLH85_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNB64211.1"
                     /translation="MLTALVQRLRPYRRRVLVAALALVLAAAAMLAVGQGLRAVIDQG
                     FAARDPAWLNRSLAATFALIALLAAATYTRFYNVSWLGERVTADLRQQLFDHLLSLPP
                     GWFEQGRTGEVMSRLTADTTQIEQVIGSSLSIALRNALLLAGGLAMLFTTSLKLTLLT
                     LAGVPLVVAPILLYGRRVRRLARESQDRVADLGNRIDETLHEIRIVQAYGHEDADRQD
                     FARLVETGFATARQRIANRARLVAAVLLLVFGAIAFILWVGGHDVLEGRLSAGELSAF
                     VFYAALVAASIGALSEVWGDLQRAAGATERIAEILATRPEIVVPANPRPLPDPPRGAL
                     HFERVRFHYPSRPDTPALDDFSLSVTPGETVALVGPSGAGKSTVFQLLLRFYDVAAGE
                     IAVDGVPLPQADPAALRRRMALVPQEPAIFAASVLENVRYARPDADEAAVRAACRAAF
                     ADEFIDRLPEGYATWLGERGQRLSGGQRQRIAIARALLADRPILLLDEATSALDSESE
                     RMVQAALDELMRGRTTLMIAHRLSTVRRAHRIVVLDHGRVVQHGSPTELLAQGGLYAR
                     LAGQQRPE"
     gene            4077..4481
                     /locus_tag="PLH85_06330"
     CDS             4077..4481
                     /locus_tag="PLH85_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNB64212.1"
                     /translation="MLSTLPLTAGVTLLIVLLMFGTCFAVGKARHTYGIQAPAMSGHP
                     AFERLYRVQMNTLEWAAMTLPALWICAAYVGDGVAAALGGVWLVGRVWYALGYARDPA
                     LRARGFGIAALAFGALGLAAGGGVIRHLLGVS"
     gene            4478..5545
                     /locus_tag="PLH85_06335"
     CDS             4478..5545
                     /locus_tag="PLH85_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HNB64213.1"
                     /translation="MRQTLRRVAVLAAIALAGPVGAAPALPALGVEGDGVTVSGISSG
                     GYMAVQFQVAFSRIVKGAGVLAAGPYECAEGSVAKALTRCMAPNRLAPPPTVADSVAR
                     VDARARAGQIDAPAGLAEDRAWVLSGGADRTVERPVVDALVAFYRHWLPATSVAYVRL
                     DDAAHGLSAGHGMISVSDAQANACATSEPPFINRCGNFDAAGELLRFLLGPLQARGDA
                     RAGRLDAFDQRPFVSGLAADAGLAEEGLVYVPDACRAGGCRLHVAFHGCRQNLDQVGR
                     RFAEGAGYAEWAATNRLVVLFPQTVTRNGWAWGSWRWVVNPKGCWDWWGYSGADYATR
                     TGPQMAAVRRMIARLAEAPGR"
     gene            complement(5603..6379)
                     /locus_tag="PLH85_06340"
     CDS             complement(5603..6379)
                     /locus_tag="PLH85_06340"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNB64214.1"
                     /translation="MSKLTTEKVLSVHHWNDSLFSFKTTRDTALRFENGQFVMIGLDV
                     EGRPLTRAYSIASPNYEEHLEFFSIKVPNGPLTSRLQHLRPGDPIVVSKKPTGTLVLH
                     DLLPGKHLYLFSTGTGLAPFLSVIQDPETYERFDKIVLLHGVRYVSELAYSEFIEDFL
                     PQNEYFGDAIRDKLIYYPTVTREEFRHQGRVTDLVTSGKLFEDIGLPPLDPAVDRAMI
                     CGSPHMLKDCCDMLDARGFKVSAHIGAPGDYVIERAFVEK"
     gene            6492..7418
                     /locus_tag="PLH85_06345"
     CDS             6492..7418
                     /locus_tag="PLH85_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB64215.1"
                     /translation="MRYTLRQLEIFVAVARHESVSRAADEIALSQSATSAALSEFERQ
                     FDTQVFDRIGKALRLNELGRLLLPEAVALLEHGRAIEGLLMRQEAFGPLQIGATLTVG
                     NYLATLIAAEFLRRHPQVRIGLQVHNSATIVQQLLRFELDLGLIEGSCRDPELIVEPW
                     VADELVVFCAPTHPLAAAGQASVDALLRQPWILREQGSGTRETFEQAMRHVTGAFDVR
                     LELEHTEAIKRAVESGLGIGCISRLALREAFRRGSLVPIEVPELDLRRQFQFVTHARK
                     YVTAGMGEFIALCRQLSAGATRSDEIELPFIP"
     gene            complement(7408..7884)
                     /locus_tag="PLH85_06350"
     CDS             complement(7408..7884)
                     /locus_tag="PLH85_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB64216.1"
                     /translation="MNLLLTARDIYRVVVAQLQRLSPLFDLAIRLYVARVFILSGLVK
                     LQSWDSTLALFENEYEVPLLPPEIAAYMGTGAELILPVFLALGLGGRFFAFALFVFNA
                     VAVLSYPDLTPAGLKDHVLWGSLLLVTLFHGPGPLAADRLLGTESPVGPTPAPVTE"
     gene            complement(7881..8657)
                     /locus_tag="PLH85_06355"
     CDS             complement(7881..8657)
                     /locus_tag="PLH85_06355"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNB64217.1"
                     /translation="MSLHELQQAFSAALLHGASADHLIAGPRGLGAADAAAIYRDALF
                     ANYRRALAASFPVVERLIGPDCFRQTADALVRAVPSTDGDVAAFGAHLPDFLADHPPT
                     RGLAWLPDVARLEWAIEMAAGAPDATPFDPAALAVDAPEALRLALHPSAWLLASPHPT
                     LAIWRANQPDRDGTVDAAAAAGGACLLVVRRPDDVHVEALTPAEFAWLAALAAGQPLG
                     DAVTAAFDTDPAFDLPATLTHHVRAGTLAGSSSAPERKLP"
     gene            complement(8644..9489)
                     /locus_tag="PLH85_06360"
     CDS             complement(8644..9489)
                     /locus_tag="PLH85_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNB64218.1"
                     /translation="MPSSAPLRSPRALAGLGLRTPHIHEILATRPPVGWLEVHSENYF
                     APGGSGIGLLERLRSHYPVSLHGVGLGLGSADPLDDTHLDRLEALIDRIDPFIVSEHL
                     CWARVAGRHLNDLLPLPYTHEALDLVCARIDAVQTRLRRRLLVENVSSYCRFHADALN
                     EWDFVAAVARRTGCALLVDVNNIYVSSRNHGFDPLAYLDALPADAVAELHLAGHEAAH
                     GCLIDTHSRPVCDAVWALYADALERFGPRPTLLERDQDIPPLAELLAEVYRAADCLEA
                     CHVPA"
     gene            complement(9556..9846)
                     /locus_tag="PLH85_06365"
     CDS             complement(9556..9846)
                     /locus_tag="PLH85_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005475027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HNB64219.1"
                     /translation="MTTITRTAPATQTVRAAVAGLLALGLAAGAMPAHAAKGDNEKCA
                     GVVKAGKNDCATASSACAGTVSKDNQKDAWIYVPKGTCEKIAGGVVVADAKK"
     gene            10065..10322
                     /locus_tag="PLH85_06370"
     CDS             10065..10322
                     /locus_tag="PLH85_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64220.1"
                     /translation="MTSRTSGIALATAAAALFATAMAAPVAQAADAPFVKCTGINSCK
                     GTSECKTAKNECKGHNSCKGMGWVKKASADECTSAGGKVMK"
     gene            10443..11285
                     /locus_tag="PLH85_06375"
     CDS             10443..11285
                     /locus_tag="PLH85_06375"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNB64221.1"
                     /translation="MSTASTIVAGFGLGLRTDHYRDFTEGRPPVDWLEILSENYMVPG
                     GKPLHFLDRIRRDYPMVMHGVSLSIGSADPLDRAYLADLKTLVERVEPAWVSDHLCWT
                     GVDHAHLHDLLPLPYTEAALRHVTERVQAVQDVLGRRLVLENVSSYVAWQADEMSEWD
                     FLAEVARRADCEILLDVNNVYVSARNHGFDPRHFIDAMPAARVRQIHLAGHEDLGDVV
                     IDTHDHPVCDDVWALYAYTVRRLGLVPTMIERDDRIPPLQTLLAELDLARRTAQGALQ
                     EAVA"
     gene            11282..12076
                     /locus_tag="PLH85_06380"
     CDS             11282..12076
                     /locus_tag="PLH85_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNB64222.1"
                     /translation="MTPLANQQRALQTAILSDVAPAGLLAGPDAARRFSIYADAYRAR
                     LAAALRDNYPVLHRVLGDEDFDALARAYLHAHPSVAPSIRWFGGALADFVQATPDALP
                     HPALVDLIRLEWAMRAAFDAEDAPPMGFATLAALPPDRWPALRLRLHPSVSRLALEWA
                     VEPLWQALAPLEDGQSPEQEPPPPEPHVHGLIAWRQGLTPCWRSVEALEGHLLAALAG
                     GAAFADLCTLAADAGEEAAAHQVAGLLRRWVDEGLLQACDALMSTP"
     gene            complement(12495..13196)
                     /locus_tag="PLH85_06385"
     CDS             complement(12495..13196)
                     /locus_tag="PLH85_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNB64223.1"
                     /translation="MIVKALIFDVFGTLVDWRTSVAREVTAHLAPQGVQVDALAFADA
                     WRDQYQPAMEAVRSGRLPFCKLDRLHRHNLDVVLADFGLDAVDESTRQSLNLAWHRLD
                     AWPDVAPGLHRLRRHYRLAPCSNGNISLMVDLARRNDFPWDAILGAEVARDYKPKPAV
                     YLAAADAFDLTPAETLMVAAHSSDLDAAARAGLRTAFIARPDEFAQGAGERMPTVPVD
                     LTAVSLTDLADQLTP"
     gene            complement(13193..14056)
                     /locus_tag="PLH85_06390"
     CDS             complement(13193..14056)
                     /locus_tag="PLH85_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB64224.1"
                     /translation="MELIALRTFEAVVEEGGILAASRKLNTVQSNVTGRIRRLEEELG
                     TELFFRKGRGLELAPPGRVLLDYARRMLQLERETSAALRQVGESVGELRIGAMETFTA
                     LHLPAALKSVRRHHPAVELRIETAPSAVLVERVLDHKLDCAMVAGPVDHPALDFCPLV
                     DEELVQICAIGTRPVHLPLILFREGCAYRTRALAWQRAMGHALSDAMEFGTVEGILGC
                     VAVGLGWTLMPRRVVAQSVHAPDLVVESVPEDIAHVPTGMIRLRGAPPMAAVDTLVAA
                     FQPIAPGGPRP"
     gene            14153..15349
                     /locus_tag="PLH85_06395"
     CDS             14153..15349
                     /locus_tag="PLH85_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNB64225.1"
                     /translation="MSSRRERFQVMGAGICSLILVLGVARFAYTPLLPLMQRQAGLGV
                     GEAGLLAAINYGGYLSGALIASLISDLVLKDRLYRIGMVLAIASTALMGLTTDVTVWA
                     VSRFVAGLSSAAGMLLGTGLILNWLIRHDHRSELGIHFSGIGLGIAGCAAAVLLMSPW
                     FDWRGQWLIFALLGGMLLVPALRWLPAPDPSPFTKSGEALRDQPPGRLFMRLFMAAYF
                     CAGVGYVVSATFIVAIVDRLPGLAGQGALVFLAIGAGAAPACIVWDFIARRTGALNAL
                     ILAAVLQIAGILLPVAVGGSLAAFVGALLFGGTFIGMVSLVLTMAGRYYPTRPAKMMG
                     KMTLSYGAAQILGPALTGWLALHWGSYTAGVYLAAGMMAVGTVLLLVLKGVERRDAAA
                     LRAVAA"
     gene            15404..17185
                     /locus_tag="PLH85_06400"
     CDS             15404..17185
                     /locus_tag="PLH85_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB64226.1"
                     /translation="MPAPVEYTIRAAHPAAHLFEVTLTLAEPDVAGQVFSLPAWIPGS
                     YMIRDFARHIVSLQAEAGGRAVAAVKDDKHTWRLPAGLRGPLTLRYTVYAWDLSVRTA
                     HLDETHGFFNGTSVFLRAHGFEDRPQRVRLLPPAGLEAAGWRVATSLPEASPEVGGAE
                     RYGFGVYEADTYDALIDHPVEMGCFDLFEFEACGVPHAVAITGRHDCDGERLVADLKP
                     VCEWQIRLFGEPAPMERYVFLVMAVGDGYGGLEHRASTALLCSRNDLPHRGMGAPTEG
                     YRQFLGLCSHEYFHTWNVKRIKPAAFVPYELGRENYTRLLWAFEGFTSYYDDLALLKS
                     GRISRREYLDLLAKTVSAVHKGSGRLKQSVAESSFEAWTKYYRQDENSPNAIVSYYTK
                     GALVALLLDLRLRGESGGACSLDSLMGVLWRDHGQTGVGVAEEGLYAAAAALGGASLG
                     RWLRNLVEGTDELPLEDGLRRVGVKLAWEASVKCPGMGARTTVEGESVKLANVFDGGA
                     AQAAGLSAGDVLVALDGLKVTGKTLDALLARRMAGETVQVHAFRRDELKTFSVILQAP
                     LPDTARLTEAPRCSAAARALREGWLGA"
     gene            complement(17233..18267)
                     /locus_tag="PLH85_06405"
     CDS             complement(17233..18267)
                     /locus_tag="PLH85_06405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB64227.1"
                     /translation="MPFIDLLKACASQLIVLHHLVIYGPLSEAMADVMPALVAWIAEY
                     GRLAVPVFLAAAGFLSARGLAPGGVPTRVQPLTLIARRYMRLAIPYLVAVTCALACAA
                     VARTWMHDDATPAIPTIRQFLAHVFLLHSLLGYDSLSAGVWYVAVDFQLYALMVGLVW
                     LAHSGRGAASPTQRLMMLVAAAAAASLLYFNLDQDWDSWGLYFFGSYALGAFAYWAAG
                     RSSHGWLVLLAAAGTLVLVVDFRVRIAVALIASLSMALACRHALFQRISPTGIVVFLS
                     KISYSTFLIHYPVSLVFNTLFANVAGHQPALAVTTFVLTWATSIGAGTLLHRYVEDGP
                     VFAVRRPVPA"
     gene            complement(18349..19785)
                     /locus_tag="PLH85_06410"
     CDS             complement(18349..19785)
                     /locus_tag="PLH85_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNB64228.1"
                     /translation="MPTPQEPAAPRIPSAEPSLARRILQLAWPVLIAQLSSMTQMVSD
                     TVLTGRYGTEDLAAVAVGSGIYIAIAMLMVGILQAVAPTVAHHVGAGRHEAIGPDLQQ
                     GFWLALLLTVPAVLFLRHPDCLMQLSHMSPAVEAKSRDYLSTIAWGLPAVLLYRTFYA
                     FANALGRTRVLMLISLAATSVHIPLSWLLINGHAGFAPLGGTGCAASSATIAWFALGC
                     AMLYLLRNPAYARYRLFTRWQAPRPAALAELLRLGLPMGFSSFVEITAFTLIALFVAE
                     LGAAVVAGHRIVANLSALIYMLPLALATATLVLVGQSAGARDDVRARQAAWCGILLAT
                     GLSTTVSVLVWLVRDRVVAAYTTDPAVGTLADALILYICLYQFFDAIQTVAAHALRGY
                     KITFGPMLVHTLCFWGIGLAGGYLLAFRGIGPFPPQGVAGFWQASVLSNIAAATLFGT
                     YLYRIARLKRPAEDAAVRQKIPYRTEKS"
     gene            complement(19820..21256)
                     /locus_tag="PLH85_06415"
     CDS             complement(19820..21256)
                     /locus_tag="PLH85_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HNB64229.1"
                     /translation="MLWCALYLPDLALQVFTRGHADATPLALLTPRPRVRVVAANPAA
                     MACGVAPGISRASALALAPGLTFAERDPDLEQAALEEIATWAGRFAGTLSLDPPGAVL
                     LEVRGSLRLFGGLPRLAEALLDGLAELGYTGRLGAAATPLAARWQARQGLSPGVGSLT
                     DDQATLDALPLTVLDDASGPGADVLELLAGLGLQRLGALRHLSRHGLARRHAQALTDC
                     LDRAYGRTPDPRPSFVPPERYSARLPLPAPTDQVDMLLFGLRRLLAGLSGWLSARQAG
                     VERFQLVLEYAGTGVETLPVILGKLANDPSRCQLLAREHLTRLLLTAPVEALRLETEA
                     PRPLAPSATDLFDAPSGPRSDPALLLATLRARLGDEAVFTCAPHADHRPEQAWRRGTP
                     RREHAPADVHTPAGPRPLWLLPRPRAVTAPAPERLLAGPERIESGWWDGAGVRRDYYV
                     ARRSDDSLVWLFQDLEPPYGWYLHGYFA"
     gene            complement(21192..21908)
                     /gene="imuA"
                     /locus_tag="PLH85_06420"
     CDS             complement(21192..21908)
                     /gene="imuA"
                     /locus_tag="PLH85_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HNB64230.1"
                     /translation="MSASARLHAVDSLPGHLVWRGDRLAVPAEAGIPSGFPDLDACLP
                     GGGWPNAALSELLIPRPGIGELGLLLPLLARTAEDHWSVCIAPPLHPYAPALAAGGVP
                     LGRILFVQTESPAHARWAARQALASRSCTAVLMWLAGTDMAALRRLQLASEDSATPLF
                     LIRPEHCATQASPAVLRLALRPRVGGVEIRILKRRGPLFAGTLALELPGPVPPRPRPH
                     ALVRPLPARSGPPGLHAGTC"
     gene            complement(22085..22687)
                     /gene="lexA"
                     /locus_tag="PLH85_06425"
     CDS             complement(22085..22687)
                     /gene="lexA"
                     /locus_tag="PLH85_06425"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNB64231.1"
                     /translation="MSEKLTARQQEILQLIRDTVEHEGRPPTRAEICTAFGFRSPNAA
                     ETHLRALAAKGVISLEEGRARGIRLTEALGLPLVGRVAAGSPILAAEHVQGRFQVDPS
                     LFSPRADYLLKVRGMSMRDAGILDGDLLAVHRTAEARNGQIVVARVDDEVTVKRLSRS
                     GPIVELLPANPDFEPIVVDTRRDLLEIEGLAVGLIRSEPL"
     gene            23024..23527
                     /locus_tag="PLH85_06430"
     CDS             23024..23527
                     /locus_tag="PLH85_06430"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNB64232.1"
                     /translation="MGQPAPLFALPDADMEIFDLASALASHHVVLYFYPRDNTPGCTR
                     QAIDFSDHDGDFARERCIVVGVSPDDCLTHADFRDHHGLSVQLLSDEDYEVCKLYNVW
                     RSQEVDGVMKQGVRRSTFIISCDGVLQHVLHDVNPRGHAAEVFKLVQQLNTRKFNANR
                     QEHGRHA"
     gene            23493..23972
                     /locus_tag="PLH85_06435"
     CDS             23493..23972
                     /locus_tag="PLH85_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB64233.1"
                     /translation="MQIAKNTVVTLDYQVRDPDGNMIDDGAHPLVYLHGGYDGIFTVI
                     EEALQGKNVGEQLNVKLQPDDAFGEYDEELVLVEDASLFPDNIEVGMSFERVGEDGED
                     EQIYRITDIADGKVVVDGNHPLAGTALVFDITIKSVRAATAEEIGHGHVHGEGGHHH"
     gene            24087..26096
                     /locus_tag="PLH85_06440"
     CDS             24087..26096
                     /locus_tag="PLH85_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB64234.1"
                     /translation="MKGQSLLQVDGLRVELRAGGRRVYPVDGVHVTVPAGQTLALVGE
                     SGCGKSMTALACMGLLPRGGRITAGQVKLAGEALERLPEVRMRDVRGRRMAMIFQEPA
                     SSLNPVMTVGDQIGEVLVRHRSLHGQPAWNEAERLLAAVGIPDPRRRLGEYPFQLSGG
                     MKQRVMIAIALAGEPELLIADEPTTALDVTIQAQVLDLLRTLQARRGMGMLLITHDLG
                     VVARMAHRVAVMYAGEVVEAGEREAFFAAPLHPYSRKLFAALPDAGRRDHALETIPGQ
                     VPPLGARLRGCRFAERCPQVRPRCRDAQPALHEVDGRQVRCHLYDAESGLSWLSAEAV
                     PAGRAATSPDSRPVLEVDGLKVHFPVRKGVFRRVTGQIKAVDGVTLSVAAGETVALVG
                     ESGCGKTTAGKALLQLVPASGGAVRYDGRELLTLAPADWRPVRRELQMIFQDPFSSLN
                     PRMRVGEILEEGMRSLGVEPDAAQRALRIEALLQRVGLDPSMRDRFPHEFSGGQRQRI
                     AIARALAVSPRLIVCDEPTSALDVSVQAQILNLLKDLQGEFGLSYLFITHNLAVVDYL
                     AHQVAVMYLGRIVERGRAEEVLRDARHPYTRMLLAAVPKVSGPVLTAEQGAGEAIDLP
                     SPLSPPAGCHFHPRCSEATVACREIYPDETRLSPTRVVRCHLYAS"
     gene            complement(26201..27634)
                     /locus_tag="PLH85_06445"
     CDS             complement(26201..27634)
                     /locus_tag="PLH85_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB64235.1"
                     /translation="MFQRVIALWVLLVGVGPALADGVPDVPLQLAQATSLSDAVGLQA
                     PKAFEVREQHVKVPTVDLTIEPNDLWDRIRRGFSMPDLNTDLVTDRQIYYINRPGALR
                     QMFSRGQRYLFHIVEELERRGMPTELALLPMVESAFNPTALSPAQASGLWQFIPSTGK
                     NYKLEQNWWVDERRDVVASTSAALDYLQTIYEMHGDWQLALASYNWGEGAVGRAIAKN
                     RAAGLPTEYQYLNMPGETRYYVPKLQALKNIIAQPELFGITLPPIANRPYFVTVESRV
                     SLDLATAARLAETPVDEILALNPGYKRPVLPKNGTQSLVIPAEKLEIFLNNLETHDPS
                     TATWRTYELQEGEGLEGVADRFGISSSRLRQVNGLSPRARIGSGYTLIVPSEAPGERI
                     ALANMLPTHPQAGAVEPPPAGRTLIQRTVVRGHNGRPMVVERRVVVTPPPAGAHRGAP
                     APGRAAAPATKSAPPAKAAPAAPHKRR"
     gene            27852..28805
                     /locus_tag="PLH85_06450"
     CDS             27852..28805
                     /locus_tag="PLH85_06450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB64236.1"
                     /translation="MRALLIEDTLTSATLVSHQLRNIGIEPILARDGERGIELFKQER
                     PDLVLLDVILPGMDGFEVARRIRQLEQAGEWTPIIFLTARTGDEDLEKGIAVGGDDYL
                     IKPVSETVLAAKVRAMQRIAQMRYSLLVLTRKLDEANQELTRLSSLDGLTAIANRRQF
                     DETLQREWRRASRQGKPVSLLFCDVDFFKQFNDGYGHQVGDECLKAVARTLKDVLRRP
                     ADMVARYGGEEFAAVLPDTDIAGALQVAEALRAAVEALGITHRFSRATKVVTISVGAA
                     TAVPARQDGSPMPLLKMADEALYAAKENGRNRVATVAPRAV"
     gene            complement(28827..29591)
                     /gene="gloB"
                     /locus_tag="PLH85_06455"
     CDS             complement(28827..29591)
                     /gene="gloB"
                     /locus_tag="PLH85_06455"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNB64237.1"
                     /translation="MDIIPLHAFSDNFIWLLRAGRHAVVVDPGDAAPVQACLAAEGLQ
                     LAAILTTHHHDDHAGGVAALTAAAPVPVYGPRDGLPVAGLTHPVGEGDVVTIPEINAT
                     FEVLHVPGHTAGHLAYYAAPALFCGDTLFASGCGRVFDGTLAQLFASLARIAALPDDT
                     QVYCAHEYTLSNLKFAAAVEPDNPAIAARVAECQALRARDLPTVPFRLGGERLVNPFL
                     RSREPAVIAAARAHGLEGDATPAAVFAAIRGWKNRF"
     gene            29626..30399
                     /locus_tag="PLH85_06460"
     CDS             29626..30399
                     /locus_tag="PLH85_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB64238.1"
                     /translation="MSPAESFPGLHAWLETPLGRYVMDWEADRHDRVVADIFGYNAVQ
                     LGIPEFDFLRANRMPFRCKAGVGDVRVQADYGALPFAANSLDLVVLPHVLEFAAHPHQ
                     VLREVERVLVADGQVVITGFNPYSLFGLRRLAAGECMGYPWHGQYLSQHRLKDWLALL
                     GFEMRLCDAGCYIPGVEQEQWIERLRLLDRAGRRWWPFMGGVYMVQAIKRVQGMRLIM
                     PRWDRKAGARALAPLVQKSTAPRAGCAQQNRNDVYFGRD"
     gene            30386..30850
                     /gene="rnhA"
                     /locus_tag="PLH85_06465"
     CDS             30386..30850
                     /gene="rnhA"
                     /locus_tag="PLH85_06465"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNB64239.1"
                     /translation="MDEIDIYTDGACSGNPGPGGWGAILRSGAHEKELWGGEPATTNN
                     RMELLAVIRALEALKRPVAARVHTDSQYVQKGISEWIHGWKARGWKTASKAPVKNEDL
                     WRALDAQAARHQVKWLWVKGHAGHVDNERADALARRGVEAVRKAGAAVEGAA"
     gene            30847..31569
                     /gene="dnaQ"
                     /locus_tag="PLH85_06470"
     CDS             30847..31569
                     /gene="dnaQ"
                     /locus_tag="PLH85_06470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNB64240.1"
                     /translation="MRQIVLDTETTGLDWKGGDRVIEIGCVEIVDRKLTGRHYHVFIN
                     PERDIDAGAIAVHGITNEFLVDKPLFRQIVEEFEAFVAGGELVIHNASFDVGFLNNEL
                     GLLGRPALTAICPSVIDTLKLAKEQNPGKKASLNALCTLYEIDNTHRSLHGALLDAEL
                     LADVYLAMTRGQESLIMALDEPPPRLDDGSGGAAASVWPPLRVILASAAEQAAHDAVL
                     AEIAKANKGHCLWIPPAPPAES"
     gene            complement(31571..32671)
                     /locus_tag="PLH85_06475"
     CDS             complement(31571..32671)
                     /locus_tag="PLH85_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB64241.1"
                     /translation="METVDCVVVGAGVVGLACARRLAQAGREVLILESEARFGSGISS
                     RSSEVIHAGLYYPPGSLRARLCVPGRDALYTYCAARAIPHRRLGKLLVATDDAQLPQL
                     DAIAACAIANGVQGLRRLSATEACALEPALTCTGALLSPDTGIVDSHALMLALLADAE
                     AAGTTLVTHVPVLGGRCSDAGIELYTGGTAPMTLRARSVINAAGLGAIRLAGALEGFP
                     ARCVPVARYAKGNYFALQGRSPFRHLIYPIPEAAGLGVHLTLDLGGQARFGPDVEWIA
                     APDYRVDSDRAAAFYPAVRRYWPALPDGALVPAHAGVRPKIHGPNEPAADFRLLGPAE
                     HGIAGLVQLFGIESPGLTCCLALADAVAAALG"
     gene            complement(32671..33423)
                     /locus_tag="PLH85_06480"
     CDS             complement(32671..33423)
                     /locus_tag="PLH85_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB64242.1"
                     /translation="MSVPIDISEEAGVRFLHFGSEWVQGAMRLRKPWSLELVYTREMM
                     AGLLLHPRPWPRKVLLIGLGAGSLARFVHHHLPDAVCTVVEIAPEVHAVARQFFRLPD
                     ENARFRVVIGDGAQYVMDTDAHWDLIAVDGFDRHARAGDLATLPFYQACWTRLSERGV
                     MAVNLFSGQRGFKGRQDAIREAFDGRLCMLPPSETGNAIALAVRGAPIELSHDALLAR
                     AAALKADTGLDLHPTLKRLDAAGGLPDGRLSV"
     gene            33507..33809
                     /locus_tag="PLH85_06485"
     CDS             33507..33809
                     /locus_tag="PLH85_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64243.1"
                     /translation="MVSFENYDSELESIDMELARLAQLCGIDLRRPGVAEQVLKGDAS
                     LCTTSNPIAWEKLRGLLVLHYHVVQEEASEEGNAVAADSVRRAVERVTARMKSGRQ"
     gene            complement(33819..34778)
                     /locus_tag="PLH85_06490"
     CDS             complement(33819..34778)
                     /locus_tag="PLH85_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64244.1"
                     /translation="MKTLLASALAGLALAAAPVVDAADGFVFGAFGDTPYNRFERLHL
                     PNLLAEMDAEPLEFVIHDGDIKNGGERCDDVLFDDRLALFQTSRNALIYVPGDNEWTD
                     CHRASNGRFDPLERLRKLRGMFFPKAGHRALGQTALALETQADSADFPEYVENQRWQI
                     GPVVFVAVNVTGSANNYGYGDAPSEEFLKRDAAVQAWLAESFARARANGAQGVVIAMQ
                     ANPDIEDFGAGRHPRGFDRLMKQLLAETRAFAGEVLLIHGDTHLHRIDQPLKDPASGE
                     TIRNFTRLETYGSPFMGWVKVAVAPGERPLFRFQSRPYTPRRD"
     gene            complement(34775..>35797)
                     /locus_tag="PLH85_06495"
     CDS             complement(34775..>35797)
                     /locus_tag="PLH85_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288082.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNB64245.1"
                     /translation="LSAQERALAREKLLYEAVLDSLAEAIPVFQRIARACALLDGLCA
                     FAEAAARYDYAMPVFSAQPGVDIRAGRHPVVERQVENFIANDCRLDATRRMLLITGPN
                     MGGKSTTMRQVALIVLLAHVGCFVPAAAARIGPVDAIFTRIGAYDDLASGRSTFMVEM
                     TEAAAILHGATERSLVLMDEIGRGTSTFDGVALAVAIARHLLDKNRCWTLFATHYFEL
                     TRLSQDYPECANVHLDAVEHNHGIVFLHALEDGPASQSYGIEVAALAGIPGSVVRDAK
                     RRLRALENREIGAGPQADLFAALPEPEAEAPAHPALTALAGIDPDSLTPREALEALYD
                     LKRLTR"
BASE COUNT         5584 a        10998 c        13553 g         5662 t
ORIGIN      
        1 cgcccttgaa gttgaagcgg ccgatgtagg cgcggctggg catctcgaac ttgccgatgg
       61 tgaggatgtc ggcgccgccg gccagctcgt cgaagacggt cttgctgccg tcgaggcagt
      121 cgcgggactg gtccacgtag gcgatcttga ccgtggggcc gatgacgacg ctgcccgagt
      181 cgggcgtgtc ctggccggtg accatcttga acagggtgga tttaccggcg ccgttggggc
      241 cgatgatgcc gacgatggcg cccggcggga tgctgaagtt caccttgtcc atcagcagct
      301 tgtcgccgaa gcccttggac acgtcggtga attcgatgac cttgtcaccg agacgctcgc
      361 ccaccgggat gaagatttcc tgggtctcgt tgcgcttctg gtattcgacg ctggacattt
      421 cctcgtagcg ggccaggcgg gccttgctct tggcctggcg ggccttgggg ttggagcgca
      481 cccattccag ttcctgcttc atggccttca tgcgggcgcc ttcctgcttg gcttcctgct
      541 ccaggcgctg ctctttctgc tccagccagc tggagtagtt gcccttccag gggatgccgt
      601 ggccgcggtc cagttcgagg atccattcgg cggcgttgtc gaggaagtag cggtcgtggg
      661 tgacggccac cacggtgccg gggaagcggc acaggaactg ttcgagccac tcgacggatt
      721 cggcgtccag gtggttggtg ggttcgtcga gcaggagcat gtcggggcgc gacaggagca
      781 gcttgcacag ggcgacgcgg cgcttttcgc cgccggagag ggggccgatc ctggcgtccc
      841 agggcggcag gcgcagggcg tcggcggcga tctcgagctg ggtttccacg tcggcgccgg
      901 aggtggcgat gatgttctcg tacttggcct ggagctcggc gagcttgtcg aagtcggcgt
      961 cgggctcggc gtaggcggcg tagacctctt ccagcttctg tttggcttcc atgacttcgc
     1021 cgaggccgga ttcgacctct tcgcgcacgg tcttggcggc gtcgagctgg ggttcctggg
     1081 gcaggtagcc gatggacatg ttgggctgcc actgcacttc gccgtcgaat tccttctcga
     1141 cgccggccat gatcttgagc accgtggatt tgcccgcgcc gttgaggccg aggaggccga
     1201 tcttggcgcc ggggaagaag gacagggaga tgtccttgat gatctgacgc ttggggggaa
     1261 cgatcttgct cacgcggagc atgctcatca cgtactgggc cattttggga tgccttcggg
     1321 ctgcaaaaac aaaggcgcaa ctttacccga gttgccccgc tttggctgcg ccggtgcggc
     1381 gccaggctca gttggccgct tgctgcatct gggcgaggag gacggcctgt tcggcgaggg
     1441 agaagaagta ggggtagagg gtgcgggtgt gcttggggga ggtgccgccg aagcgggcga
     1501 tgtggtcgtc catgtggctg atggccagat ggagcagcac cgccggggca ttgacgcgct
     1561 ggcagtggcg caggggaccg atctgcccga accccagcag gcgctggtag aacggggcgt
     1621 ggcgcgggtt gacttcgatg accacgtcgt ccgccttgtc gatgaggcgg gcctggatgt
     1681 aggccagatg aaagaggccg ccgagggctt ccttggcctg gaccggggag tccacggcga
     1741 ggccggtgat ctcgcagagc aggcgcccgc gctggcggaa ggcgtcgagt tcgtgacggt
     1801 agagcgggtc ggcctggagc ccggccgggg agtcgatgcc aaccgtgatg gtgccgaaga
     1861 cttcgtctgc gctggaggtg acgagggtgg tgcggtggtg gttgtgatgc gggccgtgat
     1921 gactgccgac cggggtgagt ccgcgccacg agtacatgcg gtcgatgagc atccggacgg
     1981 tctgcccgtg gcggaccgga tcggcccgtt cgagcgtgaa gcgtcgcggg tcctgcagat
     2041 ggacgccgcg ggggggcggt tcgacgctgt gataatggct gtggcaggct ctgtctgtca
     2101 tccgttcgag gggccgtgtc atcggtggcg tcagggattc cgcagcggag gaggccggtg
     2161 ttcccggcgt gggcaggcga gtctgaagga gccctgacga tatacgtata cagggttcgg
     2221 cacgaggccc attttggtat tgagcgcgac agatgcaacg aatcgccgaa aattaacatt
     2281 cattccggcc gctgctggcc ggcaagacgg gcgtagagcc cgccctgggc gagcagttcc
     2341 gtgggactac cgtgctgcac cacgcggccg tggtccagca cgacgatgcg gtgggcgcgg
     2401 cggacggtgg acaggcggtg ggcgatcatc agcgtcgtgc gtccgcgcat gagctcgtcc
     2461 agggcggcct gcaccatccg ctcgctttcg ctgtcgaggg ccgaggtggc ttcgtccagc
     2521 agcaggatgg ggcggtcggc taggagggcg cgggcgatgg cgatgcgctg gcgctgcccg
     2581 ccggacaggc gctggccccg ttcgcccagc caggtggcgt agccctcggg gaggcgatcg
     2641 atgaattcgt cggcgaaggc ggcccggcag gcggcgcgga cggccgcctc gtcggcatcc
     2701 gggcgggcgt agcggacgtt ttcgaggacg ctcgcggcga agatcgccgg ctcctggggc
     2761 accagggcca tgcgccggcg cagcgccgcg ggatcggcct ggggcagggg tacgccgtcg
     2821 acggcgatct cgccggccgc gacatcgtag aaacgcagca ggagctggaa gaccgtgctc
     2881 ttgccggcgc cggacgggcc caccagggcg acggtctcgc cgggggtcac ggacaggctg
     2941 aagtcgtcca gggccggcgt gtcggggcgc gacgggtagt ggaagcgcac cctctcgaag
     3001 tgcagcgcgc cgcggggcgg atcgggcagg ggccggggat tggccggtac gacgatctcc
     3061 ggtcgggtgg cgaggatctc ggcgatgcgt tcggtggcgc cggcggcccg ctgcagatcg
     3121 ccccagactt ccgacagggc ccctatgctg gccgccacca gggcggcgta gaagacgaag
     3181 gcggacaact cgccggcgct caggcggcct tccagcacat cgtggccgcc cacccacagg
     3241 atgaaggcaa tggcgccaaa gacgaggagc agcaccgccg ccaccagacg ggcccggttg
     3301 gcgatgcgct ggcgggccgt ggcgaagccc gtctccacga ggcgcgcgaa gtcctgccgg
     3361 tcggcgtctt cgtgaccgta ggcctggacg atgcggatct cgtggagcgt ctcgtcgatg
     3421 cggttgccca ggtcggcgac ccggtcctgg ctttcccggg cgaggcggcg cacgcgtcgc
     3481 ccgtacagca ggatgggggc caccaccagg gggacgccgg cgagggtcag cagggtgagc
     3541 ttgaggctgg tggtgaacag catggccagc ccgcccgcca gcagcagggc gttgcgcagg
     3601 gcgatggaca ggctggaacc gatcacctgt tcgatctgcg tcgtgtcggc ggtgaggcgc
     3661 gacatcactt caccggtgcg tccctgctcg aaccagcccg ggggcaggct caggaggtgg
     3721 tcgaagagct gctgccgcag atccgccgtg acccgctcgc cgagccagga tacgttgtag
     3781 aagcgcgtgt aggtggcggc ggcgaggagg gcgatgaggg caaaggtggc ggcgaggctg
     3841 cgattgagcc aggccgggtc gcgggcggcg aagccctggt cgatgaccgc gcgcaggccc
     3901 tgtccgaccg ccagcatggc agcggcggcc agcaccaggg ccagggccgc gacgagcacg
     3961 cggcggcggt aggggcgcag acgttggacg agggctgtca gcatgagcaa tgacggtgcg
     4021 gcgcgcggca aaggccggta ctctagcagg ccccgtgtgc ttcctttggg ttccccatgc
     4081 tttccacgct tcccctcact gccggagtga cgctgctgat cgtgctgctc atgttcggga
     4141 cctgtttcgc ggtcggcaag gcgcgtcaca cctatggcat ccaggcgccg gcgatgagcg
     4201 gacaccccgc cttcgagcgc ctctaccggg tgcagatgaa caccctggag tgggccgcga
     4261 tgaccttgcc ggccctgtgg atctgcgcgg cctacgtggg cgacggcgtc gccgcggcgc
     4321 tcggcggcgt gtggctcgtc gggcgggtgt ggtacgcgct cggctacgcc cgcgatccgg
     4381 ccctgcgggc gcggggcttc ggcattgcgg cgctggcctt cggggccctc ggcctggcgg
     4441 ctggcggcgg cgtcatccgg cacttgctgg gggtgtcgtg aggcagaccc tgcgccgggt
     4501 cgcggtcctt gcggcgatcg cgctggcggg accggtcggg gctgcgcccg ccttgcccgc
     4561 cctgggcgtg gagggcgatg gggtgacggt ctccggcatt tcatccggcg gctacatggc
     4621 ggtgcagttc caggtggcgt tttcgcggat tgtgaagggc gccggcgtgc tggcggccgg
     4681 gccctacgag tgcgcggagg gcagtgtggc gaaggccttg acgcgttgca tggcacccaa
     4741 ccgcctggcg ccgccgccga ccgtggccga cagcgtggcg cgggtggacg cccgtgcccg
     4801 tgccgggcag atcgacgcgc cggcggggct ggcggaggat cgcgcgtggg tgctctccgg
     4861 cggtgccgac cgcacggtgg agcggccggt ggtggacgcc ctggtggcct tctaccgcca
     4921 ctggctgccg gcgacctcgg tcgcctacgt acgcctcgac gacgcggccc acggcctgag
     4981 cgccggccac gggatgatct cggtgagcga cgcccaggcc aatgcctgcg ccacgtcgga
     5041 gccgcctttc atcaatcgct gcggaaattt tgacgcggcg ggcgagctgc tgcgctttct
     5101 gcttggcccc ttgcaggcgc ggggcgacgc gcgggcgggg cggctggacg ccttcgacca
     5161 gcggcccttc gtcagcggcc tggcggcgga cgcggggctg gcggaggagg gcctggtgta
     5221 cgtgccggac gcgtgccgcg cggggggctg ccggctgcac gtggctttcc acggctgccg
     5281 acagaacctg gatcaggtcg gccggcgctt tgcggaaggt gccggctatg ccgagtgggc
     5341 cgccaccaac cggctggtgg tgctgtttcc ccagaccgtg acgcgcaacg gctgggcctg
     5401 gggctcgtgg cgctgggtgg tcaatcccaa gggctgctgg gattggtggg gttattccgg
     5461 tgcagactac gcgacccgca cggggccaca gatggcggcg gtgcggcgga tgatcgcgcg
     5521 tctggccgag gcccccgggc gctgaccggc gcgtgtgcgc ccgccctgcg ccgcacggcg
     5581 cgcgggtggc gcctcgaccc cgtcactttt ccacgaaggc ccgctcgatg acgtagtcgc
     5641 cgggcgcgcc gatgtgggcc gagaccttga agccgcgggc gtccagcatg tcgcagcagt
     5701 ccttgagcat gtgcgggctg ccgcagatca tggcccggtc caccgccgga tcgaggggcg
     5761 gcaggccgat gtcctcgaag agcttgcccg aggtgaccag gtcggtgacg cggccctggt
     5821 ggcggaactc ctcgcgggtg acggtggggt aatagatcag cttgtcgcgg atggcgtcgc
     5881 cgaagtattc gttctgcggc aggaaatcct cgatgaactc ggagtaggcc agctcggaga
     5941 cgtaacggac gccgtggagc aggacgatct tgtcgaagcg ttcgtaggtc tcggggtcct
     6001 ggatcacgct caggaagggt gcgaggccgg tgccggtgga gaacaggtag aggtgcttgc
     6061 ccggcagcag gtcgtgcagc accagggtgc ccgtgggctt cttgctgacc acgatggggt
     6121 cgcccgggcg cagatgctgc aggcgcgagg tgagcgggcc gttgggcacc ttgatgctga
     6181 agaactccag gtgctcttcg tagttggggc tggcgatgct gtaggcgcgg gtgaggggac
     6241 ggccttccac gtccaggccg atcatcacga actggccgtt ttcgaagcgc agggcggtgt
     6301 cgcgggtggt cttgaagctg aacaggctgt cgttccagtg atggacggaa aggacttttt
     6361 cggtggtgag tttgctcatg gccgcctccg ggcggatggg atgacgagga cagtgtaacg
     6421 gggcggtaat tcgatagaat ggattgtata aatatgtgcg ttcgaacaaa tcgataagag
     6481 gcctgccgcc catgcgctac accctgcgtc agctggagat cttcgtggcc gtcgcccgcc
     6541 atgagagcgt gtcccgggcg gccgacgaga tcgccctgtc ccagtccgcc accagcgcgg
     6601 ccctgagtga attcgagcgg cagttcgaca cccaggtgtt cgaccgtatc ggcaaggccc
     6661 tgcgcctcaa cgagctgggc cgcctgctgc tgcccgaggc ggtggcattg ctggagcatg
     6721 gccgcgccat cgaggggctg ctgatgcgcc aggaggcctt cgggcccttg cagatcgggg
     6781 cgacgctgac ggtcggcaat tacctcgcca cgctgatcgc ggccgagttc ctgcgccgcc
     6841 atccccaggt gcgcatcggc ctgcaggtgc acaactccgc gaccatcgtc cagcagctgc
     6901 tgcgcttcga gctggatctg gggctgatcg agggctcctg ccgcgacccg gagctgatcg
     6961 tggagccctg ggtggcggac gagctggtgg tgttctgcgc gccgacgcac ccgctggccg
     7021 cggccgggca ggcgagcgtg gatgcgctgc tgcgtcagcc gtggatcctg cgggagcagg
     7081 gctccggtac gcgggaaacc ttcgagcagg ccatgcgcca cgtcaccggc gcattcgacg
     7141 tgcgcctcga gctggagcac accgaggcga tcaagcgcgc ggtggagagt ggcctgggca
     7201 tcggctgcat ctctcggctg gccctgcggg aggccttccg gcggggcagc ctggtgccga
     7261 tcgaggtgcc ggagctggat ctgcgccgcc agttccagtt cgtcacccac gcccgcaagt
     7321 acgtgaccgc gggcatgggg gagttcatcg ccctgtgtcg gcagctgagc gccggcgcca
     7381 cgcgcagcga cgaaatcgag ctgcccttca ttccgtgacc ggcgcggggg tcggcccaac
     7441 cggcgattcc gtaccgagca ggcggtctgc ggccaggggg ccggggccgt ggaagagggt
     7501 caccagcagc aggctgcccc acaggacgtg atccttcagg ccggccggcg tcaggtccgg
     7561 gtaggacagc accgcgacgg cgttgaagac gaacagggcg aaggcgaaga agcggccgcc
     7621 gagcccgagg gccaggaaga ccggcaggat cagctcggcg ccggtgccca tgtaggccgc
     7681 gatctcgggc ggcagcagcg gcacctcgta ttcgttctcg aagagggcga gggtgctgtc
     7741 ccagctctgc agcttgacca aaccggacag gatgaagacc cgcgccacgt acagacggat
     7801 ggccaggtcg aagaggggcg agaggcgttg cagctgggcg acgacgacgc ggtagatatc
     7861 gcgggcggtg aggaggaggt tcatggcagc ttcctttcgg gtgcggagga cgatccggcc
     7921 agggtgccgg cgcggacgtg gtgggtgagg gtggccggca gatcgaaggc cgggtcggtg
     7981 tcgaatgcgg cggtgacggc gtcgcccagc ggctggccgg ccgccagtgc cgcgagccag
     8041 gcgaactcgg ctggcgtgag ggcttccacg tggacgtcgt ccggacgacg gacgaccagc
     8101 aggcaggcgc cgcccgcggc cgcagcggca tcgaccgtgc catcccggtc gggctggttg
     8161 gcgcgccaga tggccagggt cgggtgggga ctggcgagca gccaggcgga cggatgcagg
     8221 gccaggcgca gggcttccgg cgcgtcgacc gccagcgcgg cggggtcaaa gggcgttgcg
     8281 tcgggggcgc cggccgccat ctcgatggcc cattcaaggc gcgccacgtc cggtagccag
     8341 gcaaggccgc gggtgggcgg atggtcggcg aggaaatccg gcaggtgggc gccgaaggcc
     8401 gccacgtcgc cgtcggtgga ggggacggcg cggaccaggg cgtcggcggt ctgacggaag
     8461 cagtccggcc cgatcagccg ctccaccacc ggaaagctgg cggcgagggc gcggcggtaa
     8521 ttggcgaaga gggcatcccg gtagatcgcc gcagcgtccg cagcgccgag gccccgcgga
     8581 ccggcgatca ggtggtcggc ggaggcgcca tggagcagcg cggcgctgaa ggcctgctgg
     8641 agctcatgca gggacatggc aggcctccag gcagtcggcg gcgcgataga cctcggcgag
     8701 gagctcggcg aggggcggga tgtcctggtc ccgctccagc agggtcggac gcgggccgaa
     8761 acgttccagg gcgtcggcgt agagggccca cacggcgtcg cacacgggac ggctgtgggt
     8821 gtcgatgagg cagccgtggg cggcctcgtg accggccagg tggagttcgg cgaccgcatc
     8881 cgccggaagg gcgtccaggt aggcaagggg gtcgaagccg tggttgcggc tgctcacgta
     8941 gatgttgttc acgtcgacga gcaatgcgca gccggtgcgc cgggcgaccg cggcgacaaa
     9001 gtcccattcg ttcagggcat cggcgtggaa gcggcagtag ctggagacgt tctccaccag
     9061 caggcggcgg cgcaggcggg tctgtacggc gtcgatgcgg gcgcacacga ggtccagggc
     9121 ttcgtgtgtg tagggcaggg gcagcaggtc gttgagatgg cgaccggcga cgcgggccca
     9181 gcagaggtgc tcggagacga tgaaggggtc gatgcggtcg atgagggctt cgaggcggtc
     9241 caggtgggta tcgtccaggg gatcggccga accgagcccg agcccgacac cgtgcaggct
     9301 gaccggatag tgactgcgca gacgctcgag cagaccgatg ccgctgccgc cgggggcgaa
     9361 gtagttttcg ctgtggactt ccagccagcc gaccggcggg cgggtcgcga ggatctcgtg
     9421 gatgtgcggg gtgcgcaggc ccaggccggc gagggcgcgg ggcgagcgga gcggggcgct
     9481 tgagggcatg aggcgacgtc cggagagagt ccgtgggcgg gcggggccgt gacggccgtc
     9541 cgcccgcgct gggaattact tcttggcgtc cgccaccacg acgccaccgg cgatcttttc
     9601 gcaggtgccc ttgggcacgt agatccaggc gtccttctgg ttgtccttgg acacggtgcc
     9661 ggcgcaggcg ctggaggcgg tggcgcagtc gttcttgccg gccttgacga cgccggcgca
     9721 cttttcgttg tcgcccttgg cggcgtgggc gggcatcgca ccggcggcga ggccgagggc
     9781 cagcaggccg gcgacggcgg cgcggacggt ctgggtggcg ggggcggtgc gggtgatcgt
     9841 ggtcatgtcg gttctcctga tggaatgaag gcttcggttt cgagcggggc gcccgtgcgc
     9901 cgccgtcgtg ggaaaagaca gggggccggc cgggatcctt acgaccggat gcgaaaaata
     9961 tttttttgcc catgccgtaa gctttggcga gggtggacgg tctcttccca tgcgagacgc
    10021 catccgggtg tcgcgtaaga cccaaccctt tcaggagact gatcatgact tcccgtactt
    10081 ccggtattgc cctggccacc gctgccgccg ccctcttcgc caccgccatg gccgcgcccg
    10141 tcgcccaggc cgccgacgcg cccttcgtga agtgcaccgg catcaacagc tgtaaaggca
    10201 cctccgagtg caagaccgcc aagaacgaat gcaagggcca caacagctgc aagggcatgg
    10261 gctgggtgaa gaaggcttcg gcagacgaat gcacctccgc tggcggcaag gtgatgaagt
    10321 aagcacggcc gaaacagcca tccgtaccgg acaccggccc tcgcgagggc gccggtgtcc
    10381 ggtcctgaat tccgcgggcg tgtgtgtgcg cgcgagcggg aaagaaagcg aaagggcgct
    10441 gtgtgagcac tgcatcgacg atcgtcgcgg gcttcgggct ggggctgcgt acggatcatt
    10501 accgggattt caccgagggc cgtccgcctg tggactggct ggaaatcctg agcgagaact
    10561 acatggtgcc cggcggcaag ccgctgcact ttctggaccg catccgccgg gactatccga
    10621 tggtgatgca cggcgtgtcc ctgtccattg gcagcgccga tccgctggat cgggcctatc
    10681 tggccgatct gaagacgctg gtggagcggg tggagccggc ctgggtgtcg gaccatctgt
    10741 gctggaccgg cgtggatcac gcccatctgc acgatctgct gccgctgccc tatacggagg
    10801 ccgcgctgcg tcatgtgacg gagcgggtcc aggcggtgca ggatgtactc ggccgccgcc
    10861 tggtgctgga aaacgtgtcc agctatgtgg cctggcaggc ggacgagatg agcgagtggg
    10921 attttctggc ggaagtggcg cggcgcgccg actgcgagat cctgctcgat gtgaataatg
    10981 tgtacgtgag tgcccgcaac cacggcttcg atccgcgcca cttcatcgac gcaatgcccg
    11041 ctgcgcgggt tcgccagatc catctggccg gccacgagga cctgggcgac gtggtcatcg
    11101 acacccacga ccacccggtg tgcgacgacg tgtgggcgct gtacgcgtat acggtccgcc
    11161 ggctcgggct ggtgccgacg atgatcgagc gggacgaccg gattccgccg ctgcagaccc
    11221 tgctggccga actggatctg gcccggcgta ccgcgcaagg cgccctgcag gaggcggtgg
    11281 catgacccct ctggcgaatc agcagcgggc cctgcagacg gccatcctga gcgacgtggc
    11341 gcccgccggc ctgctcgccg gtcccgacgc cgcacggcgc ttttccatct atgcagacgc
    11401 ctaccgggca cgcctggcgg cggcgttgcg ggacaactat ccggtgttgc accgggtgct
    11461 gggggacgag gacttcgacg cgctggcgcg cgcctacctg cacgcgcatc cgtcggtggc
    11521 cccgtcgatc cgctggtttg gtggtgcgtt ggcggacttc gtgcaggcaa cgccggatgc
    11581 ccttccccac ccggcgctgg tggacctgat ccgtctggaa tgggccatgc gggcggcctt
    11641 cgatgcggag gacgccccgc ccatgggctt tgcgacgctc gctgcgctgc cgccggatcg
    11701 ttggccggcc ctgcgcctgc gtctgcatcc ctcggtctcg cgccttgccc tggagtgggc
    11761 cgtggagccc ctgtggcagg cgcttgctcc cctggaggac ggacagtcgc ccgagcagga
    11821 gccgccacca cccgagcccc atgtccatgg tctgatcgcc tggcggcagg ggcttacgcc
    11881 gtgctggcgt agtgtggagg cccttgaagg acatctgctg gctgcgctgg cagggggagc
    11941 ggccttcgcg gatctatgca cccttgcggc ggatgcgggc gaggaggccg ccgcgcacca
    12001 ggtggccggc ctgttgcgcc gctgggtgga cgagggcctc cttcaggcgt gtgacgcgct
    12061 gatgtccacg ccgtgatgac gaagtgacaa ctacccacac ggggcgtggg caagcctgtg
    12121 gacaaactgt gggggaggcg ggcaggtggc ggacgggaaa gggattttca ggtctgccta
    12181 aaaatagtgc agatattgcg aaagccgata gcggatagtt ttttcctatc cacactccgt
    12241 ggggaatact gtgtggacaa gatgggagtc atgccctcaa gtccctgatt tattggaatc
    12301 tcgtgctgct gaccgtttca ggtcaggaac gtcgtgatgg cagcgctgcc cggttttccc
    12361 cagcattcgt gggcaagcat gtggacaagc tgtgggggcg tcgggcaatc ccttcatcgc
    12421 aaaggggttt tccagactgc cgcaaaatgg ggcatccata ccccgcgcgg tcggcggtgc
    12481 gcagggggcg acgctcaggg ggtgagctgg tcggccagat cggtcaggct gacggcggtc
    12541 aggtccaccg ggacggtcgg catccgttca ccggcaccct gggcgaactc gtcggggcgg
    12601 gcgatgaacg ccgtgcgcag gccggcgcgg gcggccgcgt ccagatcgga ggaatgggcc
    12661 gccaccatca gggtctcggc cggtgtgagg tcgaaggcgt cggcggcggc caggtagacc
    12721 gccggcttgg gtttgtagtc ccgcgccact tcggcgccga ggatggcgtc ccacgggaag
    12781 tcgttgcgcc gggccaggtc caccatcagc gagatgttgc cgttggaaca gggcgccaga
    12841 cggtagtgtc ggcgcaggcg gtgcaggccc ggcgcgacgt cgggccaggc gtcgagccgg
    12901 tgccaggcca ggttgaggga ctggcgggtg ctttcgtcca cggcatccag accgaagtcg
    12961 gcgagcacca cgtccaggtt gtggcggtgc aggcggtcga gcttgcagaa gggcaggcgc
    13021 ccgctgcgga ccgcttccat ggccggttgg tattggtcgc gccaggcgtc ggcaaaggcg
    13081 agggcgtcca cctgaacgcc ctggggagcc agatgcgccg tgacctcacg ggcgacggag
    13141 gtgcgccagt ccaccagggt gccgaagacg tcgaagatca gggctttcac gatcatggcc
    13201 gggggcctcc gggtgcgatg ggctggaagg ccgcgacaag ggtgtccacg gcggccatgg
    13261 gcggtgcgcc ccggagccgg atcatgccgg tggggacgtg ggcgatgtcc tccggtacgc
    13321 tttccacgac gagatccggg gcatgcaccg actgggcgac gactcggcgc ggcatcagcg
    13381 tccaccccag ccccaccgca acacagccga ggatgccttc gacggtgccg aattccatgg
    13441 cgtcggagag ggcgtggccc atggcccgct gccaggccag ggcgcgggtg cggtaggcgc
    13501 agccttcgcg gaacaggatc aggggcaggt gcaccggtcg ggtgccgatc gcgcagatct
    13561 ggaccagctc ttcgtccacc agcgggcaga aatccagcgc gggatgatcc accgggcccg
    13621 ccaccatggc gcagtccagc ttgtggtcga gcaccctttc cacgaggacg gcgctggggg
    13681 cggtctcgat ccgcagttca accgccggat gatgccgccg cacggatttg agggcggccg
    13741 gcaggtgcag ggcggtgaag gtctccatgg cgccgatgcg cagctcgccg acgctttcgc
    13801 ccacctggcg cagggccgcg ctggtttccc gttccagctg gagcatgcgg cgggcgtagt
    13861 cgaggaggac gcggccgggc ggggcgagtt ccagtccgcg gcccttgcgg aagaagagct
    13921 cggtgccgag ttcttcttcg aggcggcgga tgcggccggt gacgttggac tgcaccgtgt
    13981 tcagcttgcg agaggcggcc aggatgccgc cttcctcgac gacggcctcg aaggtgcgca
    14041 gggcgatgag ttccatatat ctctatagaa gaatggatgc ttcttgacaa atcatttgaa
    14101 tggatagatt acacgcctta atgtgggaac atcaacccag atggagaaaa ccatgagttc
    14161 ccgacgagag cgctttcagg tgatgggggc aggcatctgc agtctcatcc tggtgctggg
    14221 ggtggcgcgc ttcgcgtaca cgccgctgct gcccttgatg cagcgccagg ccggcctggg
    14281 cgtgggcgag gccggcttgc tcgcggcgat caactatggt ggttacctga gcggggcgct
    14341 gatcgcgtcc ctgatcagcg atctggtgct gaaggatcgc ctgtaccgca tcgggatggt
    14401 cctggccatc gccagtacgg ccctgatggg gctcaccacg gatgtgacgg tgtgggcggt
    14461 gtcccgcttc gtggcggggc tgagcagcgc ggcaggcatg ctgctcggca cggggctgat
    14521 cctcaactgg ctgatccgtc acgatcatcg gagcgagctg ggcatccatt tttcgggtat
    14581 cgggctgggg attgccggct gtgcagcggc ggtgctgctg atgagtccgt ggttcgactg
    14641 gcgcgggcag tggctgatct tcgcgctgct gggcggcatg ctgctggtgc cggccctgcg
    14701 ctggctgccg gcgccggatc cgagcccctt cacgaagagc ggcgaggcgc tgcgggacca
    14761 gccgccgggg cgactgttca tgcgcctctt catggcggcg tatttctgcg ccggggtggg
    14821 ttatgtggtg agcgccacct tcatcgtggc catcgtggat cgtctgccgg ggctggccgg
    14881 gcaaggggcg ctggtcttcc tggccatcgg tgcgggagcc gcgccggcgt gcatcgtgtg
    14941 ggatttcatc gcccgccgca ccggggcgct gaacgcgttg atcctcgcgg cggtgttgca
    15001 gatcgccggc atcctgctgc cggtggcggt gggcggcagt ctggcggcgt ttgtcggggc
    15061 gctgctcttt ggcgggacct tcatcggcat ggtgagcctg gtgctgacca tggccgggcg
    15121 ctattacccg acccggccgg ccaagatgat gggcaagatg accctctcct atggcgcagc
    15181 ccagatcctc gggccggcgc tcaccggctg gttggccctg cattggggca gttacaccgc
    15241 gggggtgtat ctggcggccg ggatgatggc ggtgggtacg gtgctgctgc tggtgctgaa
    15301 gggcgtggag cggcgcgacg cggcggcctt gcgggcggtg gcggcctgag cggcggggcg
    15361 ggtcgggtta gaatccggcc ttcccccctg cctggtccct gccatgcctg cgcccgtcga
    15421 atacacgatc cgggcggccc atccggccgc ccatctgttt gaagtcaccc tcaccctggc
    15481 cgagccggac gtggccggcc aggtcttcag cctgccggcg tggattcccg gcagctacat
    15541 gatccgggac tttgcccgcc acatcgtgag cctgcaggcg gaggcgggcg ggcgggcggt
    15601 ggcggctgtc aaggacgaca agcacacctg gcgcctgccg gccgggctgc gggggccact
    15661 cacgctgcgc tacacggtgt atgcgtggga cttgtcggta cgcaccgccc atctggacga
    15721 gacccacgga ttcttcaacg gcaccagcgt gttcctgcgt gcccacggtt tcgaggatcg
    15781 accccaacgg gtgcgtctcc tgccgcccgc ggggctggag gcggccggct ggcgggtggc
    15841 gacgagcctg ccggaggcgt cgccggaagt cgggggcgcc gagcgctacg gattcggtgt
    15901 ctatgaggcg gacacctacg atgcgttgat cgaccatccc gtcgagatgg gctgcttcga
    15961 tctgtttgag ttcgaagcct gcggcgtgcc ccacgcggtg gcgatcaccg gccgccacga
    16021 ctgcgacggc gagcggctgg tggcggatct caagccggtc tgcgagtggc agatccggct
    16081 gttcggggaa ccggcaccga tggaacgcta tgtgttcctc gtgatggcgg tgggcgacgg
    16141 ttatggcggg ctggagcacc gggcgtccac ggcgctgctg tgcagccgca acgacctgcc
    16201 ccatcgcggc atgggcgccc cgaccgaagg ctaccggcag ttcctgggct tgtgcagcca
    16261 cgagtatttc cacacctgga acgtcaaacg catcaagccc gcagccttcg tgccctatga
    16321 gctggggcgc gagaactaca cgcggctgct gtgggctttc gagggcttca cgtcctatta
    16381 cgacgatctg gccctgctga agagcgggcg gatcagccgt cgcgaatatc tggatctgct
    16441 ggcgaagacg gtgagtgctg tccacaaggg cagcggacgc ctgaagcaga gtgtggcgga
    16501 gtcgtccttc gaggcgtgga cgaagtatta ccggcaggac gagaacagcc ccaacgccat
    16561 cgtcagctat tacaccaagg gcgcgctggt ggccctgctg ctggatctgc gcctgcgggg
    16621 cgagagcggc ggggcgtgtt cactggacag tctcatgggt gtgctgtggc gagaccatgg
    16681 ccagaccggc gtcggggtgg cggaggaggg gctctacgcg gccgccgctg ccctcggcgg
    16741 cgcgtcgctc gggcgctggc tgcgtaacct ggtggaggga accgacgagc tcccgctgga
    16801 ggacgggctg cggcgcgtgg gggtgaagct ggcgtgggag gcgtcggtga aatgcccggg
    16861 aatgggcgca cggaccaccg tggagggcga atccgtgaag ctcgcgaatg tgttcgacgg
    16921 cggcgcggcc caggcggccg ggctgtcggc gggcgacgta ctggtggcgc tggacggtct
    16981 gaaggtgacc ggcaagacgc tggacgcgtt gctggcccgg cgcatggccg gtgagactgt
    17041 ccaggtccat gccttccggc gggacgaatt gaagaccttt tcggtgatct tgcaggcgcc
    17101 gttaccggat acggcccgtc tgacggaagc gccccgctgc tcggccgcgg cgcgggcctt
    17161 gcgggaaggg tggctgggcg cttgagacga cgggggccgg atctccggcc ccgcggggtg
    17221 ctgggcgggc gctcaggcgg gtaccggccg gcggacggcg aataccgggc cgtcttccac
    17281 gtaccggtgg agcagggtgc cggcgccgat gctggtggcc caggtcagca caaaggtggt
    17341 gacggccagg gcaggctgat ggccggccac attggcgaag agggtgttga agaccagact
    17401 gaccgggtag tggatcagga aggtggagta ggagatcttc gacaggaaga ccacgatgcc
    17461 ggtcggcgag atacgctgga acagggcatg acggcatgcc agcgccatgg acagggaggc
    17521 gatgagggcg accgcgatgc ggacccggaa atccaccacc agcacgaggg ttccagcggc
    17581 ggccagcagc accagccagc catgggaact gcgacccgcg gcccagtagg caaaggcgcc
    17641 cagggcgtag ctgccgaaga agtacaggcc ccagctgtcc cagtcctggt cgagattgaa
    17701 gtacagcagc gacgcggcgg cggcggccgc gaccagcatc atcaggcgtt gggtggggct
    17761 ggcggcaccg cgcccgctgt gggccagcca gaccagacca accatcaggg cgtagagctg
    17821 gaaatcgacg gccacgtacc acacgccggc ggacaggctg tcatagccga gcaggctgtg
    17881 aagcaggaag acgtgggcca ggaactggcg gatcgtcgga atggccgggg tggcgtcgtc
    17941 gtgcatccag gtgcgggcga cggcggcaca ggccagggca caggtgacgg ccaccagata
    18001 gggaatggcg agccgcatgt agcgccgggc gatcagggtg aggggctgga cgcgggtcgg
    18061 gacgccaccg ggcgccagtc cgcgggccga gaggaagccg gcggcggcca ggaatacggg
    18121 gactgccagc cggccgtatt cggcgatcca cgcgacgagg gcgggcatca cgtcagccat
    18181 ggcctcggac aagggcccgt agatgaccag atggtggagg acgatgagtt gggacgcgca
    18241 ggccttcaga agatcgatga agggcatccg gcgtgcaagt acgatgtgac ccactagagt
    18301 aaattgtgta tcaaattctt tgattttaca cggaaacatg ccggactgtc atgacttttc
    18361 cgtacggtac ggtatttttt gacgcactgc cgcatcttcg gcaggccgct tgagacgggc
    18421 aatccggtac agataggtgc cgaacagggt tgccgcggcg atgttgctga ggaccgacgc
    18481 ctgccagaat ccggccactc cctggggcgg aaaggggccg atgccgcgga aggccaggag
    18541 gtatccgccg gccagtccga tgccccagaa gcacagggtg tgcacgagca tcgggccgaa
    18601 ggtgatcttg tagccgcgca aggcatgggc ggcgacggtc tggatggcgt cgaagaactg
    18661 gtacaggcag atgtacagga tcagggcgtc ggccagggtg cccacggccg ggtcggtggt
    18721 gtaggcggcg acgacgcggt cccggaccag ccagaccagg acgctgacgg tggtggagag
    18781 tccggtggcg aggaggatcc cgcaccaggc ggcctgacgg gcgcggacgt cgtcgcgggc
    18841 gccggcggac tgtcccacca ggacgagggt ggcggtggcg agggccaggg gcagcatgta
    18901 gatcagtgcg gaaagattgg cgacgatgcg gtgaccggcg accacggcgg cgcccagttc
    18961 ggccacgaac agggcgatca gtgtgaaggc ggtgatctcg acgaagctgg aaaagcccat
    19021 cggcaggccg aggcgcagga gttcggccag tgccgcgggg cgcggcgcct gccagcgggt
    19081 gaagaggcgg taacgggcgt aggcgggatt gcgcagcagg tagagcatcg cgcagcccag
    19141 ggcgaaccag gcgatggtgg cgctggacgc ggcgcacccg gtgccgccca ggggggcgaa
    19201 tcccgcgtgg ccgttgatca ggagccagga gagggggatg tgcaccgacg tggcggccag
    19261 actgatgagc atcagtacgc gggtccggcc cagggcattt gcgaaggcgt agaaggtgcg
    19321 gtacagcagc accgcgggca ggccccaggc gatggtggag aggtagtcgc gggacttcgc
    19381 ttccaccgcc gggctcatgt gggacagctg catgaggcag tccggatggc gcaggaagag
    19441 gaccgccggc acggtcagga ggagggccag ccagaagccc tgctgcaggt ccggaccgat
    19501 ggcctcgtgc cgtcctgcgc ccacgtgatg ggcgacggtg ggggcgacgg cctggaggat
    19561 gcccaccatg agcatcgcga tggcaatgta gatcccgctg ccaacggcca cggcggccag
    19621 atcctcggta ccgtaacgtc cggtcagcac ggtgtcgctg accatctggg tcatggacga
    19681 gagctgggcg atcaggacgg gccaggcgag ctgaaggatg cgtcgcgcga gggacggctc
    19741 ggctgaaggg atgcgtgggg cggcgggttc ttggggggtg ggcatgggcg gcgagtgtaa
    19801 cccgcgtgcc ggtcggacct catgcgaaat agccgtggag gtaccagcca tatgggggct
    19861 ccagatcctg aaagagccag accaggctgt cgtcggagcg ccgcgcgacg tagtagtcgc
    19921 gccgcacgcc ggcgccgtcc caccagccgc tttcgatgcg ttccgggccg gcgaggaggc
    19981 gttccggggc gggcgccgtt acggcacggg gccgggggag cagccacagg ggacgggggc
    20041 cggcgggggt gtggacatca gcgggcgcgt gctcccgccg gggcgtgccc cgtcgccagg
    20101 cctgctcggg gcgatgatcg gcatggggtg cgcaagtgaa tacggcttcg tcgccgaggc
    20161 gggcgcgcag ggtggcgagg agcagggccg ggtcgctgcg gggcccgctg ggggcatcga
    20221 acagatcggt ggcggacggg gccagcggcc ggggggcttc ggtctccagg cgcaaggcct
    20281 cgacgggggc cgtgaggagg aggcgggtca ggtgttcgcg ggccaggagc tggcagcggg
    20341 acgggtcgtt ggccagcttg cccagaatga cgggcaaggt ttccacgccc gtgccggcgt
    20401 attccaggac cagctggaag cgttccaccc cggcctggcg ggcgctgagc cagccgctca
    20461 ggccggcgag caggcggcgc aggccgaaga gcagcatgtc cacctggtcg gtgggggccg
    20521 gcagggggag gcgggcgctg tagcgttccg gtgggacgaa gctcgggcgg ggatcaggtg
    20581 tccggccata ggcccggtca agacagtcgg tgagggcctg ggcgtggcgg cgggcgagcc
    20641 cgtggcggga caggtggcgg agcgctccga gccgctgcag ccccaggccg gcgaggagtt
    20701 ccaggacgtc ggcgccgggt cccgaggcat cgtccaggac ggtcagggga agcgcgtcga
    20761 gcgttgcctg atcgtcggtg agactgccga cgccagggga aaggccctgg cgtgcctgcc
    20821 agcgggcggc gaggggggtc gccgctgcac cgagccggcc ggtgtagccg agttcggcga
    20881 gaccgtcgag cagggcttca gcgaggcgtg ggagaccgcc gaacaggcgc aggctgccgc
    20941 gcacttccag gaggacggcg cccggtgggt ccaggctgag ggtgccggca aagcggccag
    21001 cccaggtggc gatctcttcg agcgcagcct gctcgagatc gggatcccgt tcggcaaaag
    21061 tgaggccggg ggctagggcc agagcactgg cgcggctgat ccccggcgcc acgccgcatg
    21121 ccattgccgc cggattcgcc gccaccaccc gtacgcgggg gcggggggtg aggagggcga
    21181 gcggcgtggc atcagcatgt ccccgcgtga agacctggag ggccagatcg ggcaggtaga
    21241 gggcgcacca gagcatgggg gcgggggcgc ggggggaccg ggccgggcag ttcgagagcg
    21301 agggtgccgg cgaagagggg gccgcgccgt ttcaggatgc gtatctccac gccaccgacg
    21361 cggggacgca gggccaggcg cagcacggcc ggggacgcct gggtggcgca atgttccggg
    21421 cggatgagga acaggggggt ggcgctgtcc tcggaggcca gctgaaggcg gcgcagcgcc
    21481 gccatgtcgg ttccggcgag ccacatcagg acggctgtgc agctgcggga tgcaagggcc
    21541 tgacgggcgg cccagcgggc atgcgccggg ctttcggtct ggacgaacag gatgcgtccg
    21601 aggggcaccc cgcccgctgc gagggccggg gcataggggt gcaggggcgg cgcgatgcag
    21661 acgctccagt gatcctcggc ggtgcgggcg agcaggggga gcagcagtcc gagttcgccg
    21721 atgcccggac ggggaatcag gagttcgctg agggcggcgt tgggccagcc gccaccgggc
    21781 aggcatgcgt cgagatcagg gaagccgctg gggatgcccg cttctgccgg cacggccaga
    21841 cggtcgccac gccagaccag gtggcctggc aggctgtcga cggcgtggag gcgggcggag
    21901 gcggacatgg tgtgtcaggc gcggagcgtg ctcgatggtt ggcgatgtct gtgatgcggg
    21961 cggatcaaat gcttcagaac gattggctga cgggctctgt ccgttctgcc gggccattct
    22021 ccacacgcgg ggcgtgcagg gccccgtgag ggatgtcaga ccggctgagc gcggtcggca
    22081 gcgatcagag cggctcactg cggatcaggc cgaccgcaag cccttcgatt tccaggaggt
    22141 ccctgcgggt gtcgacgacg atgggttcga aatccggatt ggcggggagg agttcgacaa
    22201 tggggccgct gcgggacagg cgcttgacgg tgacctcgtc gtccacccgg gcgacgacga
    22261 tctggccgtt gcgggcttcg gccgtgcgat ggaccgccag caggtcgccg tcgaggatgc
    22321 cggcatcgcg catgctcatg ccgcgcacct tgaggaggta gtcggcgcgg ggggagaaca
    22381 gggacgggtc cacctggaaa cgcccctgga catgttcggc tgccaggatg gggctgccgg
    22441 cggcgacccg gccgaccagg ggcaggccga gggcttcggt caggcggatg ccgcgggcgc
    22501 ggccttcttc cagggaaatg acgcccttgg cggcgagggc acgcaggtgg gtctctgccg
    22561 cattgggaga gcggaagccg aatgcggtac agatctccgc ccgggtggga ggacgcccct
    22621 cgtgctcgac ggtgtcacgg atcagctgga ggatttcctg ctggcgggcg gtgagttttt
    22681 cggacatggc agactccttt tgtgtaattt tatacagctt gtgtgctttg gcaagcggtt
    22741 tatgcaaggc cgccactttc cggccggcag accccggacg gcatgtgaca aacgtcaaac
    22801 aatgcggaca aagagcttga gcccctgcgt gacttggtct atccatcaag gacagccccc
    22861 gcagcggcca gacgggggat ccacaacgac agtgcgcgtg gcccggcagg ccacttccaa
    22921 ccccggagga gacttcatgc tggtcagtcc gtccaacccg accgccggta gagttatcca
    22981 ggcgcctcgg ttagaatcgc ccggcgtacc cgaagcccct ttcgtgggtc agcccgcacc
    23041 gctttttgcc ctgccggatg cagatatgga aatcttcgat ctcgcgtcgg cgctcgcgtc
    23101 ccatcatgtg gtcctgtact tctatccccg cgacaacacc cccggctgca cccgtcaggc
    23161 cattgatttc agtgaccacg acggagactt tgcccgggag cgctgcattg tcgtgggcgt
    23221 gagtccggac gattgcctga cacatgcgga tttccgggac catcatgggc tatcggtgca
    23281 actgctttcc gacgaagact acgaggtatg caaattgtat aacgtctggc gctcgcagga
    23341 ggtggacggc gtgatgaaac agggcgttcg ccgctcaact ttcatcattt cgtgcgatgg
    23401 tgtgctccag cacgtgctgc acgacgtcaa cccacgcggc catgctgcgg aagtcttcaa
    23461 gctggtccag caactcaaca caaggaaatt caatgcaaat cgccaagaac acggtcgtca
    23521 cgcttgacta tcaggtgcgt gatccggacg gcaacatgat cgacgacggt gctcacccgc
    23581 tggtgtacct gcatggcggc tatgacggca tcttcaccgt catcgaggag gccctgcagg
    23641 gcaagaacgt gggcgagcag ctgaacgtca agctgcagcc cgacgatgcc tttggcgaat
    23701 acgacgaaga gctggttctc gtcgaggatg catccctgtt cccggacaac atcgaagtgg
    23761 gtatgtcctt cgagcgcgtg ggcgaggacg gcgaggacga gcagatctac cgcatcaccg
    23821 acatcgccga cggcaaggtc gtggtggacg gcaatcatcc cctcgccggg accgcgctgg
    23881 tcttcgacat caccatcaag agcgtgcgtg ccgcgacggc cgaagagatc ggtcacggcc
    23941 acgtgcacgg cgaaggcggt catcaccact gatccgcaac acggtgtgcc ggaacccgaa
    24001 gggatcgacc ggcacccgga taccctgccg acgggccgct ctggcggtcc gttttctttg
    24061 cttcggacag atcgattgaa gaatccatga aaggtcagtc cctgctccag gtcgatggat
    24121 tgcgggttga actgcgtgcc ggcggacggc gtgtgtatcc cgtggatggt gtgcatgtga
    24181 cggtgccggc ggggcagacc ctggcgctgg tcggcgagtc cggctgcggc aagtccatga
    24241 cggccctcgc ctgtatgggg ctgttgccgc ggggtgggcg catcaccgca gggcaggtga
    24301 agctcgccgg cgaggcgctc gagcgtctgc cggaggtccg catgcgcgat gtgcgcggac
    24361 ggcgcatggc gatgatcttc caggagccgg cgagcagcct gaatccggtg atgacggtcg
    24421 gtgaccagat cggcgaggtg ctggtccgtc atcgcagcct gcacggccag ccggcctgga
    24481 acgaggccga gcgtctcctc gcggcggtgg gaatccccga tccgcgccgg cgcctgggcg
    24541 aatacccgtt ccagctctcg ggcggcatga agcagcgggt gatgatcgcc atcgccctgg
    24601 ccggcgagcc ggagctgctc atcgctgatg aacccaccac tgcgctggac gtgaccatcc
    24661 aggcccaggt gctcgatctg ctccgcacgc tgcaggcacg gcgcggcatg ggcatgctgc
    24721 tgatcaccca cgatctgggc gtcgtcgccc gcatggccca tcgggtggcc gtcatgtacg
    24781 cgggcgaggt ggtcgaggct ggcgaacggg aggccttctt cgcggcgccc ctgcatcctt
    24841 attcccgcaa gctgtttgcc gctttgccgg atgcggggcg gcgcgatcat gccctcgaga
    24901 ccattcccgg tcaggtccct ccgctggggg cacgcctgcg cggctgccgt tttgccgagc
    24961 gctgtccgca ggtccgcccg cgatgtcggg atgcgcagcc ggccctgcac gaggtggacg
    25021 gacgtcaggt gcgctgtcat ctttacgacg cggaatccgg cctgagctgg ctgtccgccg
    25081 aggcggtgcc ggcaggacgc gcagccacct cgccggactc ccggccggtg ctggaggtgg
    25141 atggattgaa ggtccatttc ccggtccgca agggggtatt ccggcgggtg accggacaga
    25201 tcaaggccgt ggatggcgtc accctgagtg tggcggcagg cgagacggtc gcccttgtcg
    25261 gcgaatccgg ctgcggcaag accacggcgg gcaaggcgct gctccagctg gtgcccgcca
    25321 gtggcggcgc ggtccgctac gatggtcgtg agctgctcac ccttgcgccg gccgactggc
    25381 ggccggtgcg gcgggaactt cagatgatct tccaggatcc tttcagctcc ctcaatccgc
    25441 gcatgcgcgt gggcgagatc cttgaagagg gcatgcgctc cctgggcgtg gagcctgatg
    25501 ccgctcagcg ggcgctgcgc atcgaggccc tgctgcagcg ggtcggcctg gatccgtcca
    25561 tgcgcgaccg ctttccccac gaattttcag gggggcagcg tcagcgcatc gccattgccc
    25621 gggccctcgc ggtgtcgccg cggctgatcg tctgcgatga gccgacaagt gccctggacg
    25681 tctcggtgca ggctcagatc ctcaatctcc tcaaggatct gcagggggaa ttcggcttgt
    25741 cctacctgtt catcacccat aacctggcgg tggtggatta tctggcccat caggtggcgg
    25801 tgatgtacct ggggcgcatc gtggagcgcg gtcgggccga ggaggtgctg cgggatgcgc
    25861 gccaccccta tacccgcatg ctgcttgcgg ccgtaccgaa ggtgtccggg ccggttttga
    25921 cggcggagca gggggccggt gaggcgatcg atctgccgtc tccgctgagt ccgccggcag
    25981 gctgtcattt tcatccccgc tgcagcgagg ctacggtcgc gtgccgcgag atctacccgg
    26041 acgagacccg cctgtcgccc acccgggtgg tgcgctgcca tctgtacgca tcgtgaccgg
    26101 gcggcgttga acgacaggca ggggtttgac ctctgccgcg tgtcccgccg gcgctggcgg
    26161 cacgggcagg gcgccccggg tgtcgtctcc cgactgccgt tcagcggcgc ttgtgtggcg
    26221 ctgccggtgc agccttggca ggcggcgcag acttggtcgc cggggcggcg gccctgcctg
    26281 gagcaggtgc accgcgatgg gcgcctgccg ggggcggcgt gaccaccacg cggcgttcca
    26341 ccaccatcgg ccgtccgttg tggccccgca ccacggtccg ctggatcagg gtgcgtccgg
    26401 ccgggggcgg ctcgacggca ccggcctggg gatgggtcgg cagcatattg gccagagcga
    26461 tgcgctcgcc cggcgcctcg gacggcacga tcaaggtata gccactgccg atgcgggcgc
    26521 ggggcgacag gccattgacc tggcgcaggc ggctcgacga gatgccgaag cggtcggcca
    26581 ccccttcgag gccttcacct tcctggagtt cgtaggtgcg ccaggtggcc gtggaggggt
    26641 cgtgggtttc caggttgttg aggaagatct ccagcttttc cgccggaatc accagggatt
    26701 gggtgccgtt cttgggcagg accgggcgct tgtaacccgg attgagggca aggatttcat
    26761 ccaccggcgt ctcggccagg cgcgccgcgg tggccaggtc gaggctcacg cggctctcga
    26821 cggtgacgaa atagggccgg ttggcgatgg gcggcagggt gatgccgaag agctccggct
    26881 gggcgatgat gttcttgagc gcctgcagct tgggcacgta gtagcgggtt tcccccggca
    26941 tgttcagata ctggtattcg gtaggcaggc cggcggcgcg gttcttggcg atggcccggc
    27001 cgacggcgcc ttcgccccag ttgtaggagg ccagcgccaa ttgccagtcg ccgtgcatct
    27061 cgtagatggt ctgcaggtag tcgagggccg cgctggtgga ggccaccacg tcgcggcgct
    27121 cgtccaccca ccagttctgt tccagcttgt agtttttgcc ggtggacggg atgaactgcc
    27181 acagaccgga ggcctgggcc ggggacaggg cggtgggatt aaaggcgctc tccaccatgg
    27241 gcaggagggc caactcggtg ggcatgccgc ggcgttcgag ttcctcgacg atatggaaca
    27301 ggtagcgctg gccgcgggag aacatctggc gcagggcgcc gggacggttg atgtaataga
    27361 tctgccggtc tgtcaccagg tcggtgttga ggtccggcat ggaaaagccg cggcggatgc
    27421 gatcccagag atcgttgggc tcgatggtca ggtcgacggt tggaaccttg acatgctgct
    27481 cgcggacttc aaaggccttg ggtgcctgca ggcccacggc atcggacagg gacgtggcct
    27541 gggccagttg cagcggcaca tcgggcacgc cgtcagccag ggccgggccc acacccacga
    27601 gcagcaccca cagcgcgata actctctgaa acattgggtt tcgccgcgca gggacaaagg
    27661 gcggattcta tcccccgggg gcaggagggt caaaccggcc ccggtgccat tggtccaccg
    27721 gcgagccatg aagggagagg gggacggaaa ctacggattg tggcgtgaat ccgttaaagt
    27781 aacgatttcg tacagcgggt gcccagagcg cccgccccgc ccgagaccgc ccgccaggac
    27841 cgatagccgc cgtgagagcc ctgttgatcg aggataccct gaccagcgcc acgctggtgt
    27901 cgcatcaact gcgcaatatc ggtatcgagc cgatccttgc ccgcgacggt gagcggggca
    27961 tcgagctgtt caagcaggag cgcccggacc tggtgctgct cgacgtgatc ctgcccggca
    28021 tggatggttt cgaggtggcc cggcgcatcc gccagctgga acaggcgggc gagtggacgc
    28081 ccatcatctt cctgaccgcc cgcaccggcg acgaagacct ggaaaagggc atcgcggtgg
    28141 gcggggacga ctacctcatc aagcccgtgt ctgagaccgt gctggctgcc aaggtccgtg
    28201 ccatgcagcg catcgcccag atgcgctact ccctgctggt gctgacccgc aagctcgacg
    28261 aggccaacca ggagctcacc cgcctgtcgt ccctcgatgg actcaccgcc atcgccaacc
    28321 ggcgccagtt cgacgagacc ctgcaacggg aatggcggcg agcatcccgc cagggcaagc
    28381 cggtgtcgct gctgttctgc gatgtggatt tcttcaagca gttcaacgac ggttacggcc
    28441 accaggtggg tgacgagtgc ctcaaggcgg tggcccgtac actcaaggac gtcctgcgcc
    28501 gcccggccga catggtggcc cgctatggcg gcgaggagtt tgcagccgtg ttgcccgaca
    28561 ccgacatcgc cggggccctg caggtggccg aagccctgcg ggcggccgtg gaggcgctgg
    28621 gcatcaccca ccgtttctcc cgcgcaacca aggtggtgac catcagcgtc ggggcggcga
    28681 cggccgtgcc tgcccgccag gacggcagtc ccatgccctt gctcaagatg gccgacgagg
    28741 ccctttacgc ggccaaggag aacggtcgca accgggtggc gacggtggca ccgcgggccg
    28801 tctgagggcg cccgccggct cacgcgtcag aaacgattct tccagccgcg gatcgccgcg
    28861 aagaccgcgg ccggcgtggc gtccccctcg agcccgtgag cccgcgccgc tgcgatgacg
    28921 gcgggctcgc ggctgcgcag gaagggattg acgaggcgct cgccccccag ccggaagggc
    28981 acggtcggca gatcgcgcgc ccgcaaggcc tgacactcgg cgacgcgggc ggcaatggcc
    29041 gggttgtcag gttcgacggc ggcggcgaat ttcaggttgg acagggtgta ttcgtgagcg
    29101 cagtacacct gcgtgtcgtc cggcagcgcg gcgatccgcg ccagggacgc gaagagctgc
    29161 gccaacgtgc catcgaagac ccgcccgcag ccggacgcga acagggtgtc gccgcagaac
    29221 agggcgggcg ccgcgtagta ggccagatgg ccggccgtgt ggccgggcac atgcagaacc
    29281 tcaaaagtgg cgttgatttc cgggatggtg acgacatcgc cctcaccgac cggatgggtc
    29341 aggcccgcga ccggcagccc gtcgcgcggt ccatagacgg gcaccggtgc cgccgcggtc
    29401 agcgccgcga cgccgccggc gtggtcgtcg tgatggtgcg tggtgagaat ggctgccagc
    29461 tgcagcccct cggcggccag acaggcctgt acgggcgctg catcgcccgg atcgaccacc
    29521 acggcgtggc ggcccgcgcg cagcagccag ataaagttat cgctgaaggc gtgaagaggg
    29581 ataatgtcca tccgcgcatt ctagcccctg tccccgatcc tgccgatgtc tcctgccgaa
    29641 tcctttcccg gtctgcacgc atggctcgag acaccgctcg ggcggtacgt catggactgg
    29701 gaagcggatc gccatgaccg ggtcgtggcc gacatcttcg gctacaacgc ggttcagctg
    29761 ggcattcccg aattcgattt cctgcgggcc aatcgcatgc ccttccgctg caaggcgggg
    29821 gtgggggacg tccgggtaca ggccgactat ggcgcgctgc ccttcgcggc caacagcctc
    29881 gatctggtgg tgctgcccca cgtgctggaa tttgcggccc atccgcacca ggtgttgcgg
    29941 gaggtggagc gggtgctggt ggcggacggt caggtggtca tcaccggctt caacccctac
    30001 agcctgttcg gcctgcggcg gctggctgcc ggggaatgca tgggctatcc gtggcatggc
    30061 cagtacctca gccagcaccg cctgaaggac tggctggcgc tgctgggctt cgagatgcgc
    30121 ctgtgcgacg ctggctgcta cattcccggc gtggagcagg aacaatggat cgagcgcctg
    30181 cgcctcctcg accgggcggg ccgccgctgg tggcccttca tgggcggtgt gtacatggtg
    30241 caggcgatca agcgggtgca gggcatgcgc ctcatcatgc cccgctggga ccgcaaggcg
    30301 ggcgcccggg cgctggcccc gctggtacag aaaagcacgg ccccacgcgc gggctgtgcc
    30361 caacagaaca ggaatgacgt ttattttgga cgagattgac atctacacgg acggggcctg
    30421 ctcgggcaat ccggggcccg gcggctgggg cgcgatcctg cgcagtggcg cccacgagaa
    30481 ggaactgtgg ggcggcgagc ccgccaccac caacaatcgc atggagctgc tcgccgtgat
    30541 ccgcgccctg gaagccctca aacggcccgt cgcggcccgc gtgcacacgg attcccagta
    30601 tgtgcagaag ggcatttccg agtggatcca cggctggaag gcgcgaggct ggaagactgc
    30661 ctccaaggcg ccggtcaaga acgaggatct gtggcgcgcc cttgacgccc aggccgcccg
    30721 acaccaggtc aagtggctgt gggtcaaggg ccacgcgggg catgtcgaca acgaacgggc
    30781 cgacgccctg gcgcggcgcg gcgtggaggc ggtccgcaag gcaggcgctg cagtggaggg
    30841 cgcagcatga gacagatcgt cctggatacg gaaaccaccg gcctggactg gaagggcggt
    30901 gaccgggtca tcgagatcgg ctgtgtcgag attgtggacc gcaagctcac cggccggcac
    30961 taccacgtgt tcatcaaccc ggagcgggac atcgacgcgg gcgccatcgc ggtgcacggc
    31021 atcaccaacg agttcctggt ggacaagccc ctgttccggc agatcgtcga ggagttcgag
    31081 gccttcgtgg cgggcggcga actggtcatc cacaacgcca gcttcgacgt gggcttcctc
    31141 aacaacgagc tgggcctgct cggccggccg gcgctgacgg ccatctgccc gagcgtgatc
    31201 gacaccctca agctcgccaa ggaacagaat cccggcaaga aggcgtccct caacgccttg
    31261 tgcaccctct acgagatcga caacacccac cgcagcctgc acggcgcatt gctggatgcg
    31321 gagctgctgg cggacgtcta cctggccatg acccgcggcc aggagagcct gatcatggct
    31381 ctggatgaac cgccgccgcg gctcgacgat ggcagcggcg gtgcggcggc cagcgtgtgg
    31441 ccgcccctgc gggtgatcct tgcctccgcg gccgaacagg cggcccacga cgcggtgctc
    31501 gccgagatcg ccaaggccaa caaggggcac tgtctgtgga tcccgccggc gccgccggcc
    31561 gagtcctgag tcagcccagc gccgcggcca ccgcgtcggc cagggccagg cagcaggtca
    31621 gtcccggcga ttcgatgccg aagagctgga ccagacccgc gataccgtgt tccgccgggc
    31681 cgagcaggcg gaaatcggcg gcaggctcgt tcgggccgtg gatcttcggg cgcacgccgg
    31741 catgggccgg caccagcgcc ccgtccggca gcgccggcca gtagcgccgc acggccgggt
    31801 agaaggctgc tgcgcgatcg gaatcgacgc ggtagtccgg cgcggcgatc cactccacgt
    31861 cgggcccgaa gcgcgcctga ccgcccagat ccagggtcag atgcacgccc agaccggcgg
    31921 cttccgggat ggggtagatc aggtgacgga agggactgcg gccttgcagg gcgaagtaat
    31981 tgcccttggc gtaacgggcg acgggcacgc accgcgccgg aaacccctcc agtgcccccg
    32041 ccagcctgat ggcgccgagc ccggcggcgt tgattacgct gcgggcgcgc agggtcatgg
    32101 gcgccgtgcc gccggtgtag agctcgatgc cggcatccga gcagcgtccg cccagcaccg
    32161 gcacgtgggt gaccagcgtc gtgcccgcgg cttcggcatc cgccagcagg gccagcatca
    32221 gggcatggct gtccacgatg ccggtgtccg gcgacagcag cgcgccggtg caggtgaggg
    32281 cgggctccag ggcgcaggcc tcggtggcgg acagacggcg cagcccttgc acaccgttgg
    32341 caatggcgca cgccgcaatg gcgtcgagct gcggcagctg tgcgtcgtcc gtcgccacca
    32401 gcagcttgcc gaggcggcgg tggggaatcg cccgcgccgc gcagtaggtg tacagggcgt
    32461 cgcggccggg cacgcacagg cgcgcgcgca gggagcccgg cgggtaatac aggccggcgt
    32521 ggatgacctc tgacgagcgg ctcgagatgc cactgccgaa gcgcgcctcc gattcgagga
    32581 tcagcacctc gcgacccgcc tgggccaagc gccgcgcgca ggccaggccg accacgccgg
    32641 cgccgacgac gacgcagtcg accgtctcca tcacacgctc aggcgcccgt ccggcaggcc
    32701 gccggccgcg tcgaggcgct tgagggtcgg gtgcaggtcg aggcccgtat cggccttgag
    32761 ggcggcggcg cgggcgagca gcgcgtcgtg ggacaactcg atgggcgcac cgcgcaccgc
    32821 cagggcgatg gcgttgccgg tctcggacgg tggcagcatg cacaggcgcc cgtcgaaggc
    32881 ttcgcgaata gcgtcctggc ggcccttgaa accgcgctgg ccgctgaaca ggttgaccgc
    32941 catcacgccc cgttcgctca ggcgcgtcca gcaggcctgg tagaagggca gggtggcgag
    33001 atcgccggcg cgggcgtggc ggtcgaagcc gtccacggcg atgaggtccc agtgggcgtc
    33061 ggtgtccatc acgtattggg cgccatcgcc gatcaccacc cggaagcgcg cattttcatc
    33121 tggcaggcgg aagaactggc gggccacggc atgcacttct ggagcgatct cgaccacggt
    33181 gcacaccgcg tcgggcaggt gatgatggac gaagcgcgcc agggagccag cgcccaggcc
    33241 gatcagcagc accttgcgcg gccacggccg gggatggagc agcaggccgg ccatcatctc
    33301 gcgggtatag accagctcca gcgaccaggg cttgcgcagg cgcatggcgc cctggaccca
    33361 ttccgagccg aaatggagga agcggacgcc cgcttcttca ctgatgtcga tgggtacact
    33421 catgttggaa gaggtgtatg ctgcaatgca aaattccggg gccggcagac tgcgcggttc
    33481 cgaacccgac aggacaggag tacagcatgg tcagctttga aaactacgac agcgaactgg
    33541 aatccatcga catggagctg gcgcgcctgg cccagctctg tggtatcgac ctgcgtcgcc
    33601 ccggtgtggc cgagcaggtg ctgaagggcg atgccagcct gtgcacgacg tccaacccca
    33661 tcgcctggga gaagctgcgc gggctgctgg tgctgcacta ccacgtggtc caggaagagg
    33721 cgtccgagga aggcaatgcg gtggcggccg acagcgtgcg ccgtgcggtg gagcgcgtca
    33781 ccgcccgcat gaagtccggc cgtcagtaaa gccatggctc agtcccgccg cggcgtgtag
    33841 gggcgggact ggaagcggaa cagggggcgt tcgccgggcg ccactgccac cttcacccag
    33901 cccatgaagg gcgagccgta ggtttccagg cgggtgaagt tccggatcgt ctcgccgctt
    33961 gccgggtcct tgaggggctg gtcgatgcgg tgcagatggg tatcgccgtg gatcagcagc
    34021 acctcgccgg caaaggcgcg ggtttcggca agcaactgct tcatcaggcg gtcgaagccg
    34081 cggggatggc ggccggcgcc gaaatcctcg atgtccgggt tggcctgcat ggcgatcacc
    34141 acgccctggg cgccattcgc gcgggcgcgg gcaaaggatt cggcgagcca ggcctgcacg
    34201 gccgcgtcgc gcttgaggaa ttcctcgctg ggcgcgtcgc catacccata gttgttggcg
    34261 ctgccggtga cgttcaccgc cacgaagacc accgggccga tctgccagcg ctggttctcc
    34321 acgtactccg gaaagtctgc cgagtcggcc tgggtctcca gggccagcgc ggtctggccg
    34381 agggcgcggt ggccggcctt cgggaagaac atgccgcgca gtttgcgcag gcgctccagg
    34441 ggatcaaagc ggccgttgct ggcccggtgg cagtcggtcc attcgttgtc gccgggcacg
    34501 tagatcaggg cattgcggga ggtctggaag agggcgaggc ggtcgtcgaa gaggacgtcg
    34561 tcgcagcgct cgccgccgtt cttgatgtcg ccgtcgtgga tcacgaattc caggggttcg
    34621 gcgtccatct cggccagcag attgggcagg tggaggcgct cgaagcggtt gtagggcgtg
    34681 tcgccgaagg cgccgaagac gaagccgtcg gcagcatcga cgacgggtgc ggcggcaagg
    34741 gcgaggccgg cgagggcgga ggcgagcagg gttttcatcg ggtcaggcgc ttgagatcgt
    34801 agagggcttc gagggcttcc cgcggggtca ggctgtccgg atcgatcccc gccagggcgg
    34861 tgagggccgg atgggcaggt gcttcggctt ccggctcggg cagggcggcg aacaggtcgg
    34921 cctgggggcc ggcgccgatc tcgcggtttt ccagagcgcg caggcggcgt ttggcgtcgc
    34981 gcaccacgct gccgggaata ccggccaggg cggcgacctc gatgccgtag ctctggctcg
    35041 ccgggccgtc ctccagggcg tgcaggaaga cgatgccgtg gttgtgctcc acggcgtcca
    35101 ggtgcacgtt ggcgcactcc gggtaatcct gggacaggcg ggtcagctcg aagtaatggg
    35161 tggcgaagag ggtccagcag cggttcttgt ccaacagatg gcgggcgatg gccaccgcca
    35221 gggcaacgcc gtcgaaggtg gaggtgccgc gcccgatctc gtccatcagc accaggctgc
    35281 gttcggtggc gccgtgcagg atggcggcgg cctcggtcat ctcgaccatg aaggtggagc
    35341 gcccggacgc caggtcgtcg taggcgccga tgcgcgtgaa gatcgcatcc accgggccga
    35401 tgcgcgctgc cgccgcgggc acgaagcagc ccacgtgggc gagcagcacg atcagcgcca
    35461 cctggcgcat ggtggtggat ttgccgccca tgttggggcc ggtgatgagg agcatgcgcc
    35521 gggtggcgtc caggcggcag tcgttggcga tgaagttttc cacctggcgc tccaccaccg
    35581 ggtggcggcc ggcgcgaatg tccacgcccg gctgcgcgct gaacaccggc atggcgtagt
    35641 cgtagcgggc ggcggcttcg gcaaaggcac acaggccgtc gagcagggcg caggcgcggg
    35701 cgatgcgctg aaagacgggg atcgcctcgg cgaggctgtc gagaacggct tcgtagagca
    35761 gcttctcccg cgccagggcg cgttcctggg cggacag
//
LOCUS       DAOQMM010000026        35117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_315_length_35117_cov_46.429553, whole genome shotgun sequence.
ACCESSION   DAOQMM010000026 DAOQMM010000000
VERSION     DAOQMM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 35117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35117
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_315_length_35117_cov_46.429553"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="PLH85_06500"
     CDS             complement(<1..1360)
                     /locus_tag="PLH85_06500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNB64246.1"
                     /translation="MKPSLQLKLSQHLALTPQLQQSIKLLQLSTLELNQEIEKILLEN
                     PMLERDEPEGDLGSSRVEPPGPMAPAGSEAAQEREAPAEPDREGDYDSSHEAGEWLAA
                     GSGGARDDDDESDFQEFQAAVPSLRDHLDAQIALTPLTDRDRALVRFVIEALDDDGYL
                     SQDLEDLIPLLPPELNFDLDDLQIALRQVQSLDPPGIGACDVSQCLRLQLEPRPPSPG
                     RTLALEIVAEHLELLAARDFVKLKRALRCTDDELRDAHALILSLNPRPGAQFSQADTR
                     YVVPDVVVRKIRSTWMVSLNPDAMPRLRINQLYAQILRDNRGASGSLSSHLQEARWLI
                     KNVQQRFDTIQRVSQAIVDRQRQFFDYGDVAMRPLTLREIAEQLDLHESTVSRVTTQK
                     YMATPRGIFEFKYFFGSHVSTDTGGAASSTAIRALIRQLVGAEDKKKPLSDAKIAELL
                     GQQ"
     gene            complement(1375..2097)
                     /gene="lptB"
                     /locus_tag="PLH85_06505"
     CDS             complement(1375..2097)
                     /gene="lptB"
                     /locus_tag="PLH85_06505"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNB64247.1"
                     /translation="MSLLKVSNLRKKYKARTVVHDVSFDVGSGEVVGLLGPNGAGKTT
                     CFYMIVGLVAADGGDIVLDDEVLTHRPIHQRARLGLSYLPQEMSVFRKLTVAENIQAV
                     LELQDMPKARIAERLDELLGELGIDHLRDNTSISLSGGERRRCEIARALATDPRLILL
                     DEPFAGVDPIAVIDIQKIIRFLKEKGIGVLITDHNVRETLGICDRAFIINAGEVLASG
                     RPDDIVRNEQVRQVYLGEHFRL"
     gene            complement(2084..2533)
                     /gene="recX"
                     /locus_tag="PLH85_06510"
     CDS             complement(2084..2533)
                     /gene="recX"
                     /locus_tag="PLH85_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HNB64248.1"
                     /translation="MIAPLHARALRCLAQREHSRAELARKLDGLGSADEVEAELDRLA
                     DLGLLSDARFAEAYVRAKAARFGSSRLKAELARRGVAADLIGSALHDACGGSEPERAR
                     TVLRRKFDTPPADAREWARQARFLQGRGFSTEIIRSVLKESPGDEPA"
     gene            complement(2559..3590)
                     /gene="recA"
                     /locus_tag="PLH85_06515"
     CDS             complement(2559..3590)
                     /gene="recA"
                     /locus_tag="PLH85_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNB64249.1"
                     /translation="MDDNKAKALAAALSQIEKQFGKGSIMRLGDGEVESDILTVSTGS
                     LGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKLGGTAAFIDAEHALDVT
                     YAQKLGVNIGELLISQPDTGEQALEIADMLVRSGGVDVLVIDSVAALTPKAEIEGEMG
                     DQLPGLQARLMSQALRKLTANIKRTNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRMDIRRVGTIKKNDEVIGSETKVKVVKNKVAPPFKEALFDILYGEGTSREGEIID
                     LGVTHKFIDKSGAWYAYQGDKIGQGKDNAREFLKANPALAREIENKVRVAVGLPELPG
                     VAPAAAAAA"
     gene            complement(3742..4230)
                     /locus_tag="PLH85_06520"
     CDS             complement(3742..4230)
                     /locus_tag="PLH85_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNB64250.1"
                     /translation="MMDAALDALSQRVGAFLAARGWRLVTAESCTGGWVAETVTATAG
                     SSAWFDCGFVTYSNDAKCALLGVSPATLARHGAVSEATAAEMARGALDHSDGDVSLSI
                     SGIAGPGGGTPDKPVGTVCFAWARPGATVQTATVQFDGDRETIRRQAVIHALEELLRR
                     AT"
     gene            complement(4227..4706)
                     /locus_tag="PLH85_06525"
     CDS             complement(4227..4706)
                     /locus_tag="PLH85_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNB64251.1"
                     /translation="MKPTPRFLVSDPSHFIALGFGSGLAPRAPGTFGTLLGWALFPLV
                     RAPLADVGFGLLLAACFLAGIVVCERTGRALGVADHGAIVWDEIVAIWLVLWLTPPTL
                     AWQVLAVALFRFFDIVKPPPVRWVDERTRGGFGVMLDDLLAAGYALFVLAACVRLVA"
     gene            complement(4703..5671)
                     /gene="thiL"
                     /locus_tag="PLH85_06530"
     CDS             complement(4703..5671)
                     /gene="thiL"
                     /locus_tag="PLH85_06530"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNB64252.1"
                     /translation="MASEFALIRRHFTRPARHTDLSVGDDAALIRPAAGMQLAISTDM
                     LVAGTHFFADTDPRDLGWKTLAVNVSDLAAMGATPRWVVLAASLPAADEAWVAAFAEG
                     FFACCEAFGVDAIGGDTTRGPLNLCPTVFGEVPVGQAVLRSGARPGDDLWISGTPGRA
                     ALGLAHLRGDVVLADAHLGACLAALQRPQPRVALGLALRGQATAMLDVSDGLFGDLGH
                     ILEASGVGAVVRRDALPLTDLIAACDDGERAWRACTAGGDDYELLFTAPAPRREAIAA
                     LAAPAGVSLHRIGTITADAGTLYVEGADRVRRPALLRGYDHFGEVS"
     gene            complement(5700..6176)
                     /gene="nusB"
                     /locus_tag="PLH85_06535"
     CDS             complement(5700..6176)
                     /gene="nusB"
                     /locus_tag="PLH85_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNB64253.1"
                     /translation="MSAPNKSPRRRAREFALQGVYQWLLNQSGLSAIESHMASVTGFD
                     KCDRDLFVGLLRGTLGNAEALQQAFTPFIDRSVGELSPVERAILLMGSFELVHRPETP
                     YRVVINEAIELAKGYGGTDGHKFVNGVLDKLAGRVRADEVEAAAIQRRAARQDKAD"
     gene            complement(6173..6643)
                     /gene="ribH"
                     /locus_tag="PLH85_06540"
     CDS             complement(6173..6643)
                     /gene="ribH"
                     /locus_tag="PLH85_06540"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNB64254.1"
                     /translation="MPRYDNIPEIAPSLDGQGLRIGIVMCRFNLDVCEGLLSACTAEL
                     LRLGVAPDAILIATVPGALEAPLTLQTMAKSGRYDALVALGAVIRGETYHFELVSNEQ
                     GAGITRVTLDTGVPIANGILTTEDDDQALARMQVKGADCAQAAVEMARLLKALA"
     gene            complement(6680..7774)
                     /gene="ribBA"
                     /locus_tag="PLH85_06545"
     CDS             complement(6680..7774)
                     /gene="ribBA"
                     /locus_tag="PLH85_06545"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNB64255.1"
                     /translation="MSALSPIQDIIADIKAGRMVVLVDEEDRENEGDIVLAAEHVTPD
                     AINFMAKHARGLICLTLTEARCRQLGINQMARDNRSQFSTAFTVSIEAAEGVTTGISA
                     ADRARTVQAAVARHAKPCDIVQPGHIFPIMARPGGVLIRAGHTEAGCDLAAIAGLEPA
                     SVICEIMNDDGTMARLPQLIDFAQTHGLRIGAIRDLIEYRARTEKLVTRVSEKTVDTP
                     HGAFRLCAFEDTTSGEVHLAMVHGEIRPDVETLARVHEPVSVLDFLDPAAGRHSFTVD
                     DALRRIAAHGNGVLVLLYRPQSGQDLLDQLGTTVPRPAQKWDPRLFGIGAQILRELHV
                     GKMKLMASPRKIPSMAGFGLTLSGHVTPDA"
     gene            complement(7771..8382)
                     /locus_tag="PLH85_06550"
     CDS             complement(7771..8382)
                     /locus_tag="PLH85_06550"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNB64256.1"
                     /translation="MFSGIVAATGRILDVAPLEDGVRLTVDVGALGLDDVQLGDSIAH
                     NGVCLTVIAREGNRVQFDVSRETLNCTVGLDAPGEVNLEKALRLADRLGGHLVTGHVD
                     GIGEVVKFAPIGESHELVIRAPAALAGYIARKGSITVDGVSLTVNWVKGRDFSINLIP
                     HTVQVTTLKRLAPGARVNLEIDLIARYVERMQAWRESGEEATS"
     gene            complement(8535..8966)
                     /locus_tag="PLH85_06555"
     CDS             complement(8535..8966)
                     /locus_tag="PLH85_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB64257.1"
                     /translation="MHRKLREVVAGQTVVSASASTTVRAAAVTMAQKNIGAIVVVDAA
                     GKLEGLFTERDVLNRVVARSLDPDTTPLSAVMTAKLQTASPDQPLAHALHMMFEGGFR
                     HVPVVEGGLPIGMVSARNALGPEMVEFEKELKDRDHITEIL"
     gene            complement(9057..9191)
                     /locus_tag="PLH85_06560"
     CDS             complement(9057..9191)
                     /locus_tag="PLH85_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64258.1"
                     /translation="MTRTIALLLALALGFATTAQACGDHATKNSKPDMSKPAGPKTGT
                     "
     gene            complement(9420..10373)
                     /locus_tag="PLH85_06565"
     CDS             complement(9420..10373)
                     /locus_tag="PLH85_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB64259.1"
                     /translation="MSTGNAPLQPIAFPAADGYILRGFAGCHSQAAATRPVVIINPAT
                     SVRCRYYARFATWLHGHGFDVITYDYRGIGESRPPQLRGFEAGWIDWGRLDFEGALRF
                     AAARFPGQPVHVAAHSVGGFLIGLAPSSPCVARIFTMGAQYAYWRDYARPRRAGLLLK
                     WHVAMPVLTAVFGYFPGRRLGWLEDTPRGVVRDWTARQPRFEDSWRSGARVLPEAERR
                     ALVERFAAVRAATLAVSLSDDEFGTVEAIRRLLGYYRRSDRTHLHLTPEAVGQRTVGH
                     FAFFHSRFEHSLWPIALQWLRHGHLAARPIGEVWHLAADGG"
     gene            complement(10493..13708)
                     /locus_tag="PLH85_06570"
     CDS             complement(10493..13708)
                     /locus_tag="PLH85_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64260.1"
                     /translation="MRPPSRLPSFSAQISRSFALLFTLAICAILAGWYFGVPGLGLQG
                     ERQRYIGTSIQLLEQGADFQRARLVSRLRAHRGNLLTLAENRVITGHLMARRDDDAAR
                     DIERVLERLMRAYPDDYRAVQVISAESGTLLGAVGKGQAGQAFAHPELLDTLQEPGIL
                     ELIRLLPVRGGGRDELVIARQMLAPGPDGTPGTQLVGYLVAFLNQDLLLFEDSGDDTA
                     PDEARRQTLLADTGGAVLVNTLGPGTDTTIRPLLGVSTGFEGTLRLTVAGLDEVVASY
                     RHVPINGTQGLRLVHFQRQEDMLGGVKEVTDTLVALGGAFLVLGLILVWDAARRLTRP
                     LGELLRVVDAVGAGQPAPPFEPDRADCRELAHLGTAFNEMAERVAETRLDLENRVAER
                     TAELARERSFLETVLKTVPALIWLKAPDGRYLACNAEFERFFGATEADILGRTDYDFV
                     PRELADFFREHDRRATERGGPSVNEEWVTYASDGRRVLLETTKTPLRGPDGSLLGVLG
                     IGFDITARKQAEGALLDNQRTLMAKVDERTRDLAHAKDAAEAANRAKSTFLANMSHEI
                     RTPLNAIIGLTHLLRRDQPAPRQAERLDKIDASGRHLLAIINDILDLSKIEAGKLTLD
                     DQDFALGQILDHVASLVGESAASKGLRIVIDEDHVPRWLRGDVTRVRQALLNYASNAV
                     KFSRHGTIVLRAELLQDAGDTMHVRFAVEDQGDGIPLDKQPNLFHEFEQADTSTTREH
                     GGTGLGLAITRRLAGLMGGEAGFESTPGQGSRFWFTAWLRRGRGVMPPPEAPARDTAA
                     SAEHLLRARHAGARLLLAEDNRINVEVALELLHGVSLHADVAENGRVAVEMAHSNTYD
                     LILMDMQMPEMDGLEACRQIRALPGWAARPILAMTANAFDDDRAACQAAGMNDFIAKP
                     VDPAALYATLLRWLPAAAGPIDASTATPPSVEPAPTPAAAPEDRLATAQGVDLARGLR
                     ITRNNRERYLHLLEGALDGLQQHLLTLRERLAAGDFSGAKPIAHTVKGTAGNVGLTTV
                     AAVAIELDEVLRGPDPEANRCSQLMDALDAALAEVAHALHD"
     gene            complement(13716..14777)
                     /locus_tag="PLH85_06575"
     CDS             complement(13716..14777)
                     /locus_tag="PLH85_06575"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB64261.1"
                     /translation="MRPRLLLGLLVLSIAMPALAERDTPPVGLSPASPEVDVGIQPLG
                     VPSGVISAVMSRDRILRQALESLGTPPRFHAFWRGADIVPLLADRRLEAGLLGDMPTL
                     MACARGQAVIVGLVKQTSTAIVGRRVAQVSDLAGRRIGYVEGSSAHHTLLQGLRSAGL
                     SERQVRLVPLAVSEMPEALERGEIDAFAAWEPAPSAALAANRANRIVFRGLTSDFLVL
                     DRRFAEQQPAAARHLIAGFVRALEWMRRAQANLDRAVSWARLDAQTLSGKAPPISDRQ
                     VAHITHEEILDIPSAPAIPGRSTSPPLRKEFDFLAGQGKLPPEARWDAIADALRYDGL
                     YQVLAEPRRFRLAEFDYTD"
     gene            15050..15829
                     /locus_tag="PLH85_06580"
     CDS             15050..15829
                     /locus_tag="PLH85_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64262.1"
                     /translation="MDSLSAALQRLFGRPGQPLPDPWPESGALLEPVDPDGRAWALVI
                     GLPRPAGGAMVRALCEGATALDLPVPAVSLAGDAGFHVWFALTDPVPAERGRVFLEAL
                     CRRCLPEGASRALRLPGTVGLTGLSLPPARDGGRWTAFIDPSLAGMFDGETWLDMAPS
                     PERQAELLAGFRAIRPAQFDRALAELDPPGAVVPSPRGALAGQVVEPPALSGTVPPLG
                     LGVGSGFVDPRTFLLAVMNDPGASADHRIDAAKALLPYFER"
     gene            complement(15840..16349)
                     /locus_tag="PLH85_06585"
     CDS             complement(15840..16349)
                     /locus_tag="PLH85_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNB64263.1"
                     /translation="MPAFLHRLRGHAAPLPPAPPLRAILLAGLGGFLAIAVVAGLAER
                     TAAALVLGSFGASCVLVFGFPEVPFSQPRNVVLGHVLSSAVGLACLSLAGPHWWAAAL
                     AVALAIMGMMALRCVHPPAGSNPVIVFLLQPGWSFLLLPTLAGALLLVGVALVYNNLA
                     RDADWPRYW"
     gene            16576..17181
                     /locus_tag="PLH85_06590"
     CDS             16576..17181
                     /locus_tag="PLH85_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB64264.1"
                     /translation="MTAAPPSPAESAAPMHSRSERRAHLVDTALRLFARGGYHATGID
                     AVLAASGVAKKTLYHHFPSKDDLIVEVLRERDRRFRASLAAFVAARATPRARLAAVFE
                     WHALWFAEPDFAGCMFLNAAAEFHDGSAGILAVAREHKQALEAWLAALLTDLVEAERA
                     APLAADLNLLLDGAIGTALVSRDPAAAARAWALAERLLPAK"
     gene            17193..17948
                     /locus_tag="PLH85_06595"
     CDS             17193..17948
                     /locus_tag="PLH85_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64265.1"
                     /translation="MRSACLAVILLAVAGIAHASDEARLPGDAAAVRAADLEKPFKLS
                     LGEYRYGGNFSGQDVNLRYRRGDSSAWLGSYRDRDFGTQTRAGADTSWQPFAGSSLAL
                     QPSVQLASGGFVGGSFSVELGEPWFVQAGIGRTNTRPYFNLNFDPNDAQSYAVGYRDD
                     SGQTYYLMSVRDDRLGTRQRHTHAVARWPAGPTRLTVDVLHKTGDGDNGHVDAWGATL
                     GVDYLDWFVRLVRDPHQNFGDANATRLTVGRRF"
     gene            complement(17996..18763)
                     /locus_tag="PLH85_06600"
     CDS             complement(17996..18763)
                     /locus_tag="PLH85_06600"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNB64266.1"
                     /translation="MNRRINLNADLGESFGAWTMGSDAELLATVNSANIACGFHAGDP
                     LVMRTTLRHARAAGVGVGAHPSFPDLQGFGRRPMQLPPAELEAVLIYQMAALAGMAQA
                     EGVKMGHVKPHGALSNMACTDAALARTVVAAIRDFDAGLILLAPACSALFTAGAEAGL
                     RVVAEIFADRAYQDDGSLVPRSRPDAMIHGAGASLAHVSAMLAEQALISVNGVRRPTP
                     IGSVCVHGDGPDAVATARFLRDALTRDGWALVGLDAL"
     gene            complement(18849..19874)
                     /locus_tag="PLH85_06605"
     CDS             complement(18849..19874)
                     /locus_tag="PLH85_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNB64267.1"
                     /translation="MTHLHVLDGGLGVSVQDRGRVGYRALGVPVSGALDPLLLAAANA
                     LAGAPADAAALEILLHGPRLQAADGPVRVALAGAGASRVLRGDGSAEPLPPWRSATLT
                     PGDILAVGRVEAGVAVLAVSGGMTVPVVLGSRATYARAGLGGIAGRPIQAGDRLPCGA
                     APDGPERVGRPWRLPAGPLRVIAGPQREHVDEACFAAFLAGPWTVTPAADRMGARLAG
                     PRLTHSARGAEIVTDGVTPGAIQVPADGQPILLRADCQTTGGYPKIATVIAADLPRLG
                     HLRPGDTLSFSAVDAAAAARARQEQARLFAAWRAALSALPPAGGFDATALYAANLVGG
                     VLRGDED"
     gene            complement(19871..20647)
                     /gene="pxpB"
                     /locus_tag="PLH85_06610"
     CDS             complement(19871..20647)
                     /gene="pxpB"
                     /locus_tag="PLH85_06610"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNB64268.1"
                     /translation="MPRTAPAPMSATPNAPRLLPLGDTAFTVEFGTTIAPDLHARVRG
                     FAAGLAAAADAGALPGVIEWLPAFASVTVFFDPDNADPDALAAHLLSLARQTHAVPLN
                     GATWTLPVCFDEDFGPDLDDLAARCGLDRAGVIARLTGAEFSVWMLGFLPGFPYLGGL
                     PAELAQPRLATPRTAVPAGSLAVADALCAVYPWTSPGGWRLLGRCPVPCFDVRRSARP
                     ALLAPGDRVRWQAVTRSDFDTLAADFTAGRADPDRLRAAP"
     gene            complement(20883..21575)
                     /locus_tag="PLH85_06615"
     CDS             complement(20883..21575)
                     /locus_tag="PLH85_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNB64269.1"
                     /translation="MRTLRGILKALLLLVICTQAWAFGLADLSDKDASGGLREALSQG
                     ASKAVGLLGTDGGFLNNPKVKIGLPESLAPVEGVLRTMGRGKDLDSLVATMNHAAEQA
                     VPKAKVLLVDAVKKMSVDDAKKILGGGDDAATQYFKEKTSAQLFESFLPTVKATTDKL
                     ALSQQYNALAGKAGSFGLGKGDDLKVENYVTRKALDGLFLMIAEEEKAIRKDPLGTAT
                     GLAKKAFGALGQ"
     gene            21606..22100
                     /locus_tag="PLH85_06620"
     CDS             21606..22100
                     /locus_tag="PLH85_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB64270.1"
                     /translation="MLPKAITRFIAALLFLALLDGCSAPAAPDVSFTTLDGQTTRLSA
                     LRGKVVLVNFWATTCTTCVGEMPKLVDTYRRFAPRGFETVAIAMSYDPPEYVRNYTAK
                     AGLPFKVALDARGEAARGFEEVRLTPTTFLLDKQGRVVQKYLGEPDFPKLHALLEKLL
                     ADPA"
     gene            22097..23332
                     /locus_tag="PLH85_06625"
     CDS             22097..23332
                     /locus_tag="PLH85_06625"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB64271.1"
                     /translation="MKRPVLSVALLAAAGLAALVLWPRGREVDGVAVRRAPLTQSVVA
                     TGRIATPARITVGAPLAATVLAISVREGDSVKPGQVLARLQADDADALVRQAAAALAE
                     AEARLSQIATVGEPVAAQALAQAEANLHVARREAERSRQLVAQGFYSQSRVDDALRIE
                     TNARNALDTTRAQLRAQQQGGTEREAARARVDQARAQLANARARAALLTLTAPSAGTV
                     LSRKAEPGDVTTAGKVLLELAEAGETRVYATVDEKNLRLLALGQKARGVADAFPGTPF
                     EAELYYLAPAVDPQRGTVEIRFRVPAPPAFLRPDMTISVETVTGHRDATLVLPSEALR
                     EADGGAPWVLVARDGVAERVPVQTGLTGIGQVEILHGLVEGDVAILPASGALDGDRVR
                     LREMKKPRNTLQVPTGMAR"
     gene            23856..25076
                     /locus_tag="PLH85_06630"
     CDS             23856..25076
                     /locus_tag="PLH85_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB64272.1"
                     /translation="MTLRLPFAPLVALRFLREGRMQTLLILAGTTGGVAVIVFLTQLI
                     TQLQAQIVDRVLGSQAHVVIRPLEEANRRAFAPDGMATVVEPRAQRLRSIDQWESVAR
                     LAAGLPGVTAVSPVATGPAFATRGQANKSIALMGVDADRYRRIVRMEEYMKAGRFAVD
                     GTRAVIGADLATDLGLRLEDKLRVMLPNGRDELLQVGGIFDIGNKDLNRRWVFVTLPL
                     AQSLLDLPGGVSNLDLTVADIFEARPIAERLRGATGLTVDSWMETNAQLLAALRNQTV
                     SNNLIRSFVVLIVALGIASVLVVSVVQKQKEIGILRAMGARRGMILQVFLIQGAVVGA
                     VGSVLGSALAFTLLRVFSSIYRNADGSPLFSPQLELRFIVMAALVATAVGLLSALIPA
                     RRAARMDPVQAIRA"
     gene            25073..25777
                     /locus_tag="PLH85_06635"
     CDS             25073..25777
                     /locus_tag="PLH85_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB64273.1"
                     /translation="MSALRPAAAGPVLELAGITKTYNPGTPVATEVLHGIDLSLAEGE
                     FTALVGPSGSGKSTLLNLIGLLERPSAGRLLIAGRDTGQLDEAALTALRGRSIGFVFQ
                     FHHLLPAFTAHENVMMPALIAHGVADPQIEADALALLDAVGLKAHAHKKPAQLSGGQQ
                     QRVAIARALALRPRLILADEPTGNLDTRTADDIFALLREFNHQTRCACLIVTHDPRLA
                     ARCDRTVTLVDGRLVI"
     gene            complement(25869..26465)
                     /locus_tag="PLH85_06640"
     CDS             complement(25869..26465)
                     /locus_tag="PLH85_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64274.1"
                     /translation="MRRPFVFILATLCSAPLAVLPAAAHARERLAHPNPAGGTTARHV
                     DAGIGPHGTTFAHGRRSVSDGTGQISSTRAGVFRGAEGASAAHASRLTREADGALTRD
                     SRFAARSARGSLDSQGSVSRAADGTLYGDRSTTAQGSAGGSYAGSTHYDQDSVSHSSA
                     ATRDDGTRYTGTTAYSRGEGVSHSGGCSDPSGKPVACR"
     gene            26673..28046
                     /locus_tag="PLH85_06645"
     CDS             26673..28046
                     /locus_tag="PLH85_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNB64275.1"
                     /translation="MSDTRELAKEALGTFESAIMGIAGTAPAFSVAVTTAAIVASVGV
                     LSVGSILYCGLIMFGIMLAFVHLSKISAHAGAAYAWVGEVFGPVWGFFTGWGVLVAST
                     FFMVSATIPAATSTLVILMPDKVEDTLWVTSTAALWLTAVTAVVTKGIKHASYAQVIL
                     TVIETVVVLALIVAAFLEYGSRPAHPPSMIWFSPFAFTPQIFATGALTAIFFYWGWDV
                     TMNLSEESKQAAGGHTAAGQGAFWAMVNLILFFVIMMIVVLIVLNDAEIAEANTNVLY
                     AIANKLFAPPWNYLAVLSTILSTVGTIETQILQFSRSMFAMARDEMLHPRYARIHPEW
                     QTPWVATVVIWGLGVVLLFASSALPSVKVILQSSILAIGFQICFYMSLAGFACAWHYR
                     AKLGEGLKAATSYVLWPLGGALFMVFIAVYSVPTFDLITNVSGIGGLLIGAIPLILNR
                     KRVMAGA"
     gene            complement(28116..28445)
                     /locus_tag="PLH85_06650"
     CDS             complement(28116..28445)
                     /locus_tag="PLH85_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNB64276.1"
                     /translation="METLDPLLHQPLRTQLAAFLAGAGEASFSELKRGLQVSDGNLES
                     HLKKLVAAGYVSVARDTGPGRPQTRYALTAEGSEALRTYVQTLQRLLPLAEPNLPDTA
                     GGTLADA"
     gene            complement(28457..28855)
                     /locus_tag="PLH85_06655"
     CDS             complement(28457..28855)
                     /locus_tag="PLH85_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64277.1"
                     /translation="MHRLAALALVSLVLPGCASLIRPSDAELAQLPVVRFGDAAPAGQ
                     PYILHYPAGAPLPVEARIHGSLMEQDTRSTLQVSIRKDIYTYKQWASLDGKTWCRATD
                     LIGGHFQFVVPGEGDSRAPGVLRAEFNLKP"
     gene            complement(28881..29930)
                     /locus_tag="PLH85_06660"
     CDS             complement(28881..29930)
                     /locus_tag="PLH85_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64278.1"
                     /translation="MADPIARQLDAIQSMLLRGQRNLRMERHSLILWGLAGALLFACS
                     DTVLTAEQIPDASRRATAWLLFISTVLGGVGVADWHLTRRVKQARDETWSFIHRQVVK
                     LLWLLMGVGTLFTFATFFYGGAYMLCTVWLVLIGLALYVHGLFSEEVLEWAGGTIILI
                     GVAGLVARLPFETMKWIAIAVFGLGLPLLSALLDHGRTRSFGVRLAQSAGWTVLVLAA
                     PLAAHRYASTSLPPELPVMSLAEFMAGGTMTGERIVQLPAGTRVPVRVQVSGDLFRPD
                     PDAVLPLVLDRPVELLLRDGALTGDVRAPGGPWQLARETRWISIPWIRAGLEAGRGPS
                     VDTALVVDFHGHPFH"
     gene            30310..30990
                     /gene="bluB"
                     /locus_tag="PLH85_06665"
     CDS             30310..30990
                     /gene="bluB"
                     /locus_tag="PLH85_06665"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HNB64279.1"
                     /translation="MSDTPPQRFSDADIAAVYRAIAERRDMRHFRPDPVAPALLERLL
                     WAAHHAPSVGYMQPWRFIRITDRALRERLHALVEVERCATARALGEREDEFMRLKVEG
                     VREAGEVIVTALADGRERHIFGRRTLPEMDLASVACAIQNMWLAARAEGLGLGWVSIF
                     DPAEVATLLGLPEGARAVAILCIGHVEAFYPRPMLEMEQWAERAPLATVVYENAWGRT
                     GTGPADPA"
     gene            complement(31036..32019)
                     /locus_tag="PLH85_06670"
     CDS             complement(31036..32019)
                     /locus_tag="PLH85_06670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HNB64280.1"
                     /translation="MFKAILIEKDEAGYRAGVRELDDSALPEGDVTVRVAYSCLNYKD
                     GLAITGKGPVVRKFPLVAGIDLAGTVEASSHPGIAVGDAVVLNGWGVGEGHWGGLAQR
                     ARLRGDWLIPLPGAFTPRQAMAIGTAGYTAMLCVMALERHGVTPAQGEVLVTGANGGV
                     GSVAIALLAKLGYTVVASTGRLDEAGFLKSLGAAEVIDRAPFSAPGRPLAKERWAGAV
                     DTVGSHTLANVCASMRYRGTVAACGLAQGMDLPATVAPFILRGVTLAGVDSVMCPRAE
                     RIEAWNRLARDLDMSKLDLITHEIGLAEAIPTAAALLAGQVRGRVVVDVNR"
     gene            complement(32121..32639)
                     /locus_tag="PLH85_06675"
     CDS             complement(32121..32639)
                     /locus_tag="PLH85_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB64281.1"
                     /translation="MSSPPLPYLLGYPEAVRTQAAQALATGRLGPWLQQRYPDTHGLR
                     SDTALYDYVAALRNRFLRTAPPLAKVRYDGTLQTVHQALGTHTRVTRVHGGRLRTKRE
                     IRIATLFRVAPEPFLHMIAAHELAHLREPEHDKAFYQLCCHMAPDYAQLELDVRLYLT
                     HLDAAGAPLWTP"
     gene            complement(32709..33443)
                     /locus_tag="PLH85_06680"
     CDS             complement(32709..33443)
                     /locus_tag="PLH85_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNB64282.1"
                     /translation="MASEFQLSVCPHDTAKNLLGWFTLNTYLQRKLGIGIHFEPQDNF
                     LVERQRVLDARFHVVYANPYSALCFARDKGFVPVARPAGVVDEAVIVARTEGAVPDAP
                     TVASATDKLIIHDLGLQVLRTLGIDPAAARFEFAGNHLNAAKAVIDGRADLGIVFNET
                     WAGLSPATRASLRVLGESTDGVAFHCFMVSPEWADKRADIQAILCGMHEDPAGRKVLD
                     DLRFSAFAPVGEECLPPLQALLAAKG"
     gene            33607..34023
                     /locus_tag="PLH85_06685"
     CDS             33607..34023
                     /locus_tag="PLH85_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64283.1"
                     /translation="MSGRGAGGRAPFAYLTRLPPGKAVLWCYLIWYLSTVAHHFDPSP
                     KLWLNALGISGVIGIALMLSVAGTTRPDRWQTARLFMMPFGVSSFSALIKGQGFVLIF
                     PPTWAECAVSAGACALFLAVVAAAKALVRQPGTTPQ"
     gene            complement(34017..34295)
                     /locus_tag="PLH85_06690"
     CDS             complement(34017..34295)
                     /locus_tag="PLH85_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64284.1"
                     /translation="MLKTVRQFVFARAWLTFVVLALSFVAFGIASLNLVTLLKANLAL
                     FLEHGSMAAMDGALGQLLDLLLSTVLSMVFYLVFKSCEYALVRRIGGY"
     gene            complement(34353..>35117)
                     /locus_tag="PLH85_06695"
     CDS             complement(34353..>35117)
                     /locus_tag="PLH85_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HNB64285.1"
                     /translation="DKHGTFLFRPYKPSYILPVRWSDARNPTPTSPRLGTAATSGTLD
                     SVEAKYQLSFKTKLWEGLFGRHGDLWLGYTQQSSWQVYNKGNSSPFRETDYEPELMAV
                     FRTNRDLGAGFRLRLLSIGLNHQSNGRAGDLSRSWNRVMAQAGVERGDFSLIVRPWVR
                     LHENAGNDDNADITHYLGHGDVQAVWRFSQQTLGLNLRGNPTAGRGSAGVDWSFPIHR
                     NLKGYLQVFSGYGETLIDYNHRQNVIGFGVSLVDWM"
BASE COUNT         5086 a        11662 c        12956 g         5413 t
ORIGIN      
        1 cctgctggcc caacaactcg gcaatcttgg cgtcggacaa gggttttttc ttgtcctcgg
       61 ctcccaccag ctggcgaatc agcgcccgaa tcgccgttga cgaggccgcc ccaccggtgt
      121 cggtggaaac gtgactgccg aaaaaatatt tgaactcgaa gataccgcgc ggcgtggcca
      181 tgtacttttg ggtggtcacc cgcgacaccg tggattcgtg tagatccagc tgctcggcga
      241 tttcccgcag ggtaagcggg cgcattgcca catcgccata atcgaagaac tgacgctggc
      301 ggtcaacgat ggcttgcgac acgcgctgga tcgtgtcgaa gcgctgctgt acgttcttga
      361 tcagccagcg tgcctcctgc agatggctgg acagggagcc gctggcgccc cggttgtcgc
      421 gcaggatctg ggcatacagc tggttgatgc gcaggcgagg catggcgtcc ggattgaggc
      481 tcaccatcca ggtgctgcgg atcttgcgca cgaccacatc cggcaccacg tagcgggtat
      541 cggcctggga gaactgcgcg ccaggtcgcg gattgagact gaggatcagg gcatgggcgt
      601 cgcgcagctc atcgtcggtg cagcgcaggg cgcgcttgag cttgacgaag tcccgcgcgg
      661 cgagcagttc gagatgctcg gcgacgatct ccagcgccag cgtacggccc ggagacggcg
      721 gccgcggttc gagctgcagg cgcaggcact ggctcacgtc acaggcgccg atgccgggcg
      781 gatcgaggct ttgcacctgt ctgagggcga tctgcagatc atccaggtcg aaattgagtt
      841 cgggcggaag cagagggatc aggtcttcca ggtcctggct caggtagccg tcgtcgtcga
      901 gggcctcgat gacgaagcgc accagcgccc gatcccgatc ggtcagcgga gtcagggcga
      961 tctgggcgtc caggtggtcg cgcagggagg gtacggcggc ctggaattcc tgaaagtcgg
     1021 actcgtcgtc atcgtcccgt gcgccgccgc tgccggcggc cagccattca ccggcctcgt
     1081 ggctgctgtc gtagtcccct tcccggtcgg gttcggccgg ggcctccctt tcctgggcgg
     1141 cctcgctgcc ggccggcgcc atgggcccgg ggggctcgac gcggcttgag ccgagatccc
     1201 cttccggctc gtcccgttcg agcatgggat tctcgaggag gatcttttcg atttcctggt
     1261 tgagctcgag ggtggacagc tgcagcagct tgatggattg ctgcagctgc ggggtcagcg
     1321 cgaggtgctg ggaaagcttg agctggagac tgggcttcat gagtcgggac gctctcagag
     1381 ccggaaatgc tcgcccagat agacctggcg gacctgctcg ttgcggacga tgtcgtccgg
     1441 gcgcccgctg gcgagcactt cgccggcgtt gatgatgaag gcccggtcgc agatgccgag
     1501 ggtttcccgg acgttgtggt cggtgatgag gacgccgatc cccttttcct tgaggaaacg
     1561 gatgatcttc tggatgtcga tgacggcgat ggggtccacg ccggcgaagg gttcgtcgag
     1621 gaggatcagg cgcggatcgg tggccagggc gcgggcgatc tcgcagcggc gacgttcgcc
     1681 gccggacagg gagatcgacg tgttgtcgcg caggtggtcg atgcccagct caccgaggag
     1741 ttcgtcgagg cgttcggcga tgcgggcctt gggcatgtcc tgaagctcca gcaccgcctg
     1801 gatgttttcg gcgaccgtga gcttgcggaa cacgctcatt tcttgcggca ggtaggacag
     1861 accgaggcgc gcgcgctggt ggatggggcg gtgggtgagt acctcgtcgt cgaggacgat
     1921 gtccccgcca tctgccgcca cgaggccgac gatcatgtag aagcaggtcg tcttgcctgc
     1981 gccgttgggc cccagcaggc cgaccacttc gccactgccg acgtcaaagg acacgtcatg
     2041 gacgacggtg cgcgccttgt atttcttgcg cagattgctg actttaagca ggctcatcgc
     2101 cgggggattc tttcaggaca ctacggatga tttcggtcga aaaaccgcga ccttgaagga
     2161 aacgggcctg acgtgcccat tctcgcgcgt ccgcgggggg tgtgtcgaac tttctccgca
     2221 gcaccgtgcg ggccctttcg ggttcggagc caccgcaggc gtcgtggagg gcgctgccga
     2281 tcaggtccgc tgccacgccc cggcgggcca gttctgcctt gaggcgactg ctgccgaagc
     2341 gggccgcctt ggcgcgcacg taggcttcgg cgaagcgggc gtcggagagc aggcccaggt
     2401 ccgcgaggcg gtcgagttcc gcttccacct cgtcggcgct gcccagccca tcaagcttgc
     2461 gcgcgagttc ggcgcgggag tgctcgcgct gggccagaca gcgcagggcc cgggcgtgga
     2521 ggggggcgat catccgcaat gcgcccgact gaacgttctc aggcagctgc ggcggcggcg
     2581 ggcgccaccc ccggcagctc gggcagcccg acggcgacac ggaccttgtt ctcgatctcg
     2641 cgggcaaggg ccgggttggc cttgaggaat tcccgggcgt tgtccttgcc ctgaccgatc
     2701 ttgtcgccct gataggcgta ccaggcgccc gatttgtcga tgaacttgtg ggtgacgccc
     2761 agatcgatga tctcgccctc gcgggaggtg ccctcgccgt agaggatgtc gaacagggcc
     2821 tccttgaacg ggggcgcgac cttgttcttg acgaccttga ccttggtttc ggagccgatc
     2881 acttcgtcgt tcttcttgat ggtgccgaca cggcggatgt ccatgcgcac ggaggcgtag
     2941 aacttgagcg cgttgccgcc ggtggtggtc tcggggctgc cgaacatcac gccgatcttc
     3001 atgcggatct ggttgatgaa gatcaccagg gtgttggtgc gcttgatgtt ggcggtgagc
     3061 ttgcgcaggg cctggctcat caggcgggcc tgcaggccgg gcagctggtc gcccatctcg
     3121 ccttcgattt cggccttggg cgtgagggcg gccaccgagt cgatgaccag cacgtccacg
     3181 ccgccggagc gtaccagcat gtcggcgatc tcgagggcct gttcgccggt gtcgggctgg
     3241 gagatgagga gctcgccgat attcacgccg agcttctggg cgtaggtgac gtccagcgcg
     3301 tgttcggcgt cgatgaaggc ggcggtgccg ccgagcttct gcatctcggc aatgacctgc
     3361 agtgtgagcg tggtcttgcc ggaggattcc gggccgtaga tttccaccac gcggccgcgg
     3421 ggcaggccgc cgatgccgag ggcgatgtcg aggccgaggg agccggtgga gacggtgagg
     3481 atgtcgcttt ccacctcgcc atcgccgagg cgcatgatcg agcccttgcc gaactgcttt
     3541 tcgatctggg agagcgcggc ggcgagcgcc ttggccttgt tgtcgtccat cttggtattc
     3601 cttgcgaaag gttgtgcgga ttatggcaca gagtttgcgt gcgggtggac gcggcgccgg
     3661 agcggcatcg cgataagtga tattttatac agtgatatgc cggcgtccag cacaatcgtg
     3721 cggatcgccc gcccgcggac gtcaggtggc gcgccgcagc agctcttcga gggcgtggat
     3781 caccgcctgg cgacggattg tttcccggtc gccgtcgaac tggacggtgg cggtctggac
     3841 ggtggctccg gggcgcgccc aggcgaagca cacggtgcct accggcttgt ccggcgtgcc
     3901 gccgcccggg ccggcgatgc cggagatgga caggctcacg tcgccgtcgc tgtggtccag
     3961 cgcaccgcgc gccatttcgg cggcggtggc ttcgctcacc gcaccgtggc gtgccagggt
     4021 cgcgggcgag acgcccagca gggcgcactt ggcatcattg gaataggtga cgaagccgca
     4081 gtcgaaccag gccgagctgc ccgccgtggc ggtgacggtt tcggccaccc agccgccggt
     4141 gcaggattcc gcggtgacca ggcgccagcc gcgggcggcg aggaaggcgc cgacccgctg
     4201 cgacagggcg tccagggcgg catccatcag gccaccagcc gcacgcaggc ggccaggacg
     4261 aagagcgcgt agcccgctgc cagcaggtcg tccagcatca ccccgaagcc gccgcgggtg
     4321 cgctcgtcca cccagcggac gggcggcggt ttgacgatat cgaagaagcg gaacagcgcc
     4381 accgccagca cctgccaggc cagggtcggt ggcgtcagcc acagcaccag ccagatggcg
     4441 acgatctcgt cccacacgat ggcgccgtga tcggccacgc ccagggcgcg cccggtgcgt
     4501 tcgcacacca cgatgccggc caggaagcag gcggccagca gcaggccgaa acccacgtcg
     4561 gccaggggcg cacggaccag cgggaacagc gcccagccca gcagggtgcc gaaggtgcca
     4621 ggcgcccgtg gcgccagccc ggagccgaaa ccgagggcga tgaaatggga cgggtcggac
     4681 accaggaatc gcggcgtcgg cttcatgaga cctcaccgaa gtgatcgtag ccgcgcagca
     4741 gggcgggccg gcggacgcgg tcggcgcctt cgacgtacag cgtgcccgcg tccgcggtga
     4801 tcgtgccgat gcggtgcagc gacacgccgg ccggagcggc cagcgcggcg atggcctccc
     4861 gccggggagc gggcgcggtg aacagcagtt cgtagtcgtc gccgccggcg gtgcaggcgc
     4921 gccaggcccg ctcgccgtcg tcacaggcag cgatgaggtc cgtcaggggg agcgcgtccc
     4981 gccgcacgac ggcgcccacg ccggatgctt caaggatgtg gccgagatcg ccgaagagcc
     5041 cgtcggagac gtcgagcatg gcggtcgcct gtccgcgcag ggcgaggccg agggcgaccc
     5101 gcggctgggg tcgctgcagc gcggcgaggc aggcgccgag gtgggcgtcc gccagcacca
     5161 cgtcgccgcg caggtgggcg aggccgaggg cggcgcggcc gggcgtgccg gagatccaca
     5221 ggtcgtcgcc cggccgggcg ccgctgcgca ggacggcttg cccgacgggc acctcgccga
     5281 agacggtggg gcacaggttg agggggccgc gggtggtgtc gccgccgatg gcgtccacgc
     5341 cgaaggcctc gcaacaggcg aagaaaccct ccgcgaaggc ggcgacccag gcctcgtcag
     5401 cggccggcag gcttgcggcg aggaccaccc agcgtggcgt ggcacccatc gcggcgagat
     5461 cggagacgtt gaccgcgagg gtcttccagc ccagatcccg cgggtcggtg tcggcgaaga
     5521 agtgggtgcc ggccaccagc atgtcggtgg agatggccag ctgcatgccc gccgcggggc
     5581 ggatcagggc cgcgtcatcg cccaccgaca ggtcggtgtg gcgggccgga cgggtgaaat
     5641 ggcgccggat cagggcaaac tcggaggcca tggcggattc cgcgtcaccc ggcagggcgt
     5701 cagtcggcct tgtcctggcg cgcggcgcgg cgctggatgg cggcggcttc cacttcgtcg
     5761 gcgcggaccc ggccggcgag cttgtcgagc acgccgttca cgaacttgtg gccgtccgtg
     5821 ccaccgtagc ccttggccag ttcgatggct tcgttgatga cgacccggta gggggtttcc
     5881 gggcgatgga ccagttcgaa gctgcccatc aggaggatcg cccgttccac cggggaaagt
     5941 tcgccaacgc tgcggtcgat gaagggcgtg aaggcctgct ggagcgcctc ggcgttgccc
     6001 agggtgccgc gcaggaggcc gacgaagagg tcccggtcgc acttgtcgaa gccggtgacg
     6061 ctggccatgt ggctttcgat ggcgctcagg ccggactggt tgagcagcca ctgatacacg
     6121 ccctgcaggg cgaattcgcg ggcacggcgg cggggcgact tgttgggcgc gctcatgcca
     6181 gggccttcag cagtcgggcc atttccacgg cggcctgggc gcagtcggcg cccttgacct
     6241 gcatgcgggc gagggcctgg tcgtcgtcct cggtggtgag gatgccgttg gcgatgggca
     6301 cgccggtgtc cagggtcacg cgggtgatgc cggcgccctg ctcattggaa accagctcga
     6361 agtggtaggt ctcgccgcgg atcaccgcgc cgagggcaac cagcgcgtcg tagcgaccac
     6421 tcttggccat ggtctgcagc gtgaggggcg cttcgagggc gcccggcacg gtggcgatga
     6481 ggatggcgtc cggcgccacg cccaggcgca gcagttcggc ggtgcaggcc gacagcagac
     6541 cttcgcacac gtccaggttg aagcggcaca tgacgatgcc gatgcgcagg ccctggccgt
     6601 cgaggctggg ggcgatttcg gggatgttgt cgtaacgggg catgggatgt cctgcggaaa
     6661 agagcgaggg cggccgcgct caggcgtcgg gcgtgacgtg gccgctgagg gtgaggccga
     6721 agccggccat gctggggatc ttgcgtgggc tggccatcag cttcatcttg ccgacgtgca
     6781 gctcacgcag gatctgggcg ccgatgccga acaggcgcgg gtcccacttc tgggccggcc
     6841 gcggcaccgt ggtgccgagc tggtcgagca gatcctggcc gctctggggg cggtagagga
     6901 gcaccagcac gccgttgccg tgagcggcga tgcggcgcag ggcgtcgtcc accgtgaagc
     6961 tgtggcggcc ggcggcgggg tcgaggaagt ccagcaccga gacgggttca tggacacggg
     7021 cgagggtctc cacgtccggg cggatctcgc catggaccat ggccaggtgc acctcgccgc
     7081 tggtggtgtc ctcgaaggca cacaggcgga aggcgccgtg gggcgtgtcc acggtcttct
     7141 cgctgacccg cgtgacgagc ttttcggtgc gcgcgcggta ttcgatcagg tcgcggatgg
     7201 cgccgatcct gagcccgtgg gtctgggcga agtcgatgag ctggggcagg cgggccatgg
     7261 tgccgtcgtc gttcatgatc tcgcagatca cggacgccgg ctccagcccg gcgatagccg
     7321 ccaggtcgca gcctgcctcg gtgtggccgg cgcggatcag cacgccgcca gggcgcgcca
     7381 tgatcgggaa gatgtggccc ggctggacga tgtcgcacgg cttggcgtgg cgcgccacgg
     7441 cggcctgcac ggtgcgcgcc cggtcggccg ccgagatgcc ggtggtgacg ccctcggcgg
     7501 cttcgatgga gaccgtgaag gcggtggaga actggctgcg gttgtcccgc gccatctggt
     7561 tgatgcccag ctggcggcag cgcgcttcgg tgagggtcag gcagatcagg ccgcgggcgt
     7621 gcttggccat gaagttgatg gcgtccggcg tgacgtgctc ggcggcgagg acgatgtcgc
     7681 cctcgttttc ccggtcttcc tcgtccacga ggacgaccat gcggccggcc ttgatgtcgg
     7741 cgatgatgtc ctggatcggc gacagggcgc tcacgatgtc gcctcctcgc cggattcgcg
     7801 ccaggcctgc atgcgttcca cgtagcgggc gatcaggtcg atctccagat tgacccgcgc
     7861 gccgggcgca aggcgcttga gggtggtgac ctggaccgtg tgggggatca ggttgatgga
     7921 gaagtcgcgg cccttgaccc aattgacggt gaggctgacg ccatccacgg tgatcgagcc
     7981 cttgcgcgcg atgtagccgg ccagcgccgc aggggcgcgg atgaccagtt cgtggctttc
     8041 gccgatgggc gcgaacttca ccacctcgcc gatgccgtcc acgtggccgg tgaccaggtg
     8101 gccgcccagg cggtcggcca ggcgcagggc cttttcgagg ttcacctcac cgggggcgtc
     8161 gaggcccacg gtgcagttga gggtttcgcg ggagacatcg aactggacgc ggttgccctc
     8221 gcgggcgatg acggtgaggc acacgccgtt gtgggcgatg ctgtcaccga gctggacgtc
     8281 gtccagcccg agggcgccga cgtcgacagt gaggcggacg ccgtcttcaa ggggcgcgac
     8341 gtccaggatg cggccggtgg cggcaacgat tccggagaac atggcttggg gggaggtgat
     8401 gggggaaagt cgcgatttta ggcgatttcc gtccacggca ggcagtttgt gcggcgcggg
     8461 tcggtccatc gccgccgcgg ctcataaacg cgaaaagccc cggacggggc ttttccggtg
     8521 gctgggcgga aggctcagag gatctcggta atgtgatccc ggtccttgag ttccttctcg
     8581 aattcgacca tttccggacc cagggcgttg cgcgccgaga ccatcccgat gggcagcccg
     8641 ccttcgacga ccggcacatg gcggaagcca ccctcgaaca tcatgtgcag ggcgtgggcc
     8701 agaggctggt ccggggaggc ggtctgcagc ttggcggtca tcaccgcgga gagcggggtg
     8761 gtgtccggat ccagactacg ggcgacgacg cgattgagca cgtcccgctc ggtgaacagg
     8821 ccttcaagct tgccggccgc gtccacgacg acgatcgcgc cgatgttctt ctgggccatc
     8881 gtcaccgcgg cagcacggac ggtggtggat gcgctggcgg atacgacggt ctgacccgct
     8941 acgacttcgc gcagttttcg atgcatggtt gtctcctgat gggggtttga tggggaaaac
     9001 ggtgcggggg aacgacgcac aaaaggggcg ctcctggcgc cccgtgcttg cgacgttcag
     9061 gtccctgtct tgggaccggc cggcttgctc atgtctggct tggaattctt ggtggcgtgg
     9121 tcgccgcagg cctgggcggt cgtggcgaaa cccagcgcga gcgccagcag cagtgcgatc
     9181 gtacgagtca tgacggtgtc tcctccgtga tgcaacgatg cttcatcatg gaacggatcg
     9241 cagggatgcg caagaggggg gcgcgcgcgg ccgcgcggtc tcccagtcgc gatgccggtc
     9301 agttccggaa gggctgtcac ggggaccggt cgtcgtgctc aggcaacccg tcggaggggt
     9361 gccggacccg cgccgtccgt acggtgggcg ccagacggaa gtctgccgcg ccggttccgt
     9421 caaccgccgt cggcggcgag atgccacacc tcgccgatgg gccgggcggc gagatgcccg
     9481 tggcgcagcc actgcaacgc gatgggccac aggctgtgtt cgaagcggct gtgaaagaac
     9541 gcgaagtggc cgacggtccg ctgtccgacc gcctccgggg tgaggtgcag atgcgtgcgg
     9601 tcgctgcgcc ggtagtagcc gagcagccgg cggatggcct cgacggtccc gaattcgtca
     9661 tcgctgaggc tcacggcgag ggtcgcggcg cgtaccgcgg caaagcgttc gacgagggcg
     9721 cggcgttcgg cttccggcag gacccgcgcg ccgctgcgcc agctgtcttc aaagcggggc
     9781 tggcgggcgg tccagtcgcg caccacgccg cgcggcgtgt cttccagcca accgaggcgc
     9841 cgacccggga aatagccgaa gacggcggtg agcacgggca ttgccacgtg ccacttgagg
     9901 agcagaccgg cacgccgcgg tcgggcgtag tcgcgccaat aggcatattg ggcacccatt
     9961 gtgaagatcc gcgccacgca cgggctggac ggtgccagtc cgatgaggaa gccgccgacg
    10021 ctgtgggccg ccacgtgcac cggctggccg ggaaaacggg ccgccgcgaa gcgcagggcg
    10081 ccctcgaaat cgagccggcc ccagtcgatc cagccggcct cgaagccgcg caattgcggc
    10141 gggcgcgatt cgccaatgcc gcggtaatcg taggtgatga cgtcaaagcc gtggccgtgc
    10201 aaccaggtgg cgaagcgagc gtagtagcgg cagcgcacgg aggtggcggg attgatgatc
    10261 accaccgggc gggtcgctgc ggcttgggaa tggcatccgg caaaaccgcg caggatgtag
    10321 ccatccgcgg cggggaaggc gatgggctgc agtggggcgt tgccggtgga catgggcgga
    10381 tgcggcgagg ctgggacggg ggaatgctag gtcggccgat ccggtcgcgc aaacggaact
    10441 ccggcatggg cgtcatgcgg tctgcgcatg ggcgctccgc gcgcgggtgg cgtcagtcgt
    10501 gcagggcgtg ggccacttcg gcgagcgccg cgtcaagggc gtccatcagc tggctgcagc
    10561 ggttggcctc gggatccggc ccccgcagca cctcgtccag ttcgattgca accgccgcca
    10621 cggtggtgag ccccacgttg ccggcggtgc ccttgactgt atgggcaatg ggcttggcgc
    10681 ccgagaagtc gcccgcagca aggcgttcgc gcagggtcag caggtgctgc tgcagaccgt
    10741 ccagggcgcc ctcgagcaga tggaggtagc gctcgcggtt gttacgggtg atgcgcaggc
    10801 cgcgggccag gtccacgccc tgtgcggtgg cgaggcggtc ctcgggggct gctgcgggcg
    10861 tgggggccgg ctccacggat ggcggtgtcg cggtggaagc gtcgatcgga cctgccgccg
    10921 ctggcagcca gcgcagcagc gtggcgtaaa gggcggccgg atccaccggt ttggcgatga
    10981 agtcgttcat gcccgccgcc tgacaggccg cccggtcgtc atcgaaggcg ttggcggtca
    11041 tggccaggat cggccgcgca gcccagccgg gcaatgcacg gatctgccgg caggcttcga
    11101 ggccgtccat ctcgggcatc tgcatgtcca tcaggatgag gtcgtacgtg ttgctgtggg
    11161 ccatctccac ggcgacgcgg ccgttttcgg ccacgtccgc gtggaggctg acgccatgga
    11221 gcagctccag cgcgacttcg acgttgatcc ggttgtcctc cgccaggagc aggcgggcgc
    11281 cggcatggcg ggcgcgcagc aggtgctcgg ccgaggcagc ggtgtcccgg gccggggcct
    11341 cgggcggcgg catgacgccc cggccgcggc gcagccaggc ggtgaaccag aagcggctgc
    11401 cctgcccggg tgtgctttcg aagcccgctt cgccgcccat caggccggcc aggcgtcgcg
    11461 tgatagccag cccgaggccg gtgccgccat gctcgcgggt ggtgctggtg tcggcctgct
    11521 cgaattcgtg gaacaggttg ggctgcttgt cgagggggat gccgtcgccc tgatcctcca
    11581 cggcgaagcg tacgtgcatg gtgtctccgg cgtcctgcag cagctcggcg cgcaggacga
    11641 tggtgccgtg acgggagaac ttgaccgcat tgctggcgta gttgaggagg gcctgacgga
    11701 cgcgggtgac atcgccccgc agccagcgcg gcacgtggtc ttcgtcgatg acgatgcgca
    11761 ggcctttgga cgctgcggat tcgccgacga gggaggcaac gtggtcgagg atctggccga
    11821 gggcgaagtc ctgatcgtcg agggtgagct tgccggcctc gatcttggag aggtcgagga
    11881 tgtcgttgat gatggcgagc aggtggcggc ccgaggcgtc gatcttgtcg agccgctcgg
    11941 cctggcgggg ggcgggctgg tcgcggcgca gcaggtgggt gaggccgatg atggcgttga
    12001 ggggcgtgcg gatttcgtgg ctcatgttgg ccaggaaggt gctcttggcc cggttggcgg
    12061 cttcggcggc atccttggca tgggcgaggt cgcgggtacg ctcgtccacc ttggccatca
    12121 gggtgcgctg gttgtcgagc agggcgcctt cggcctgttt gcgggcggtg atgtcgaagc
    12181 cgatcccgag cacgccgagg aggctgccgt cgggaccccg caggggcgtc ttggtggtct
    12241 ccagcaacac gcgccggccg tcgctggcgt aggtgaccca ttcttcattg acggacggac
    12301 cgccccgctc cgtggcgcgg cggtcgtgct cgcggaagaa gtcggccagt tcgcggggta
    12361 cgaagtcgta gtcggtgcgg cccaggatgt cggcttcggt cgcaccgaaa aagcgctcga
    12421 attcggcgtt gcaggccaga tagcggccgt cgggggcctt cagccagatc agtgcaggga
    12481 cggtcttcag cacggtctcg aggaagctgc gctcccgcgc cagctcggcc gtgcgttcgg
    12541 cgacgcggtt ttccaggtcg aggcgggtct ccgcgacccg ttcggccatc tcgttgaagg
    12601 ccgtgcccag atgggccagt tcgcggcagt ccgcccgatc gggctcgaac ggaggcgccg
    12661 gttggccggc gccgaccgcg tccaccaccc gcagcagctc gccaagcggt cgcgtcaggc
    12721 ggcgggcggc gtcccacacc aggatcagcc ccagcacgag gaaggcgccg cccagggcga
    12781 cgagcgtgtc ggtgacctcc ttgacgcctc cgagcatgtc ttcctgacgc tggaagtgga
    12841 cgaggcgcag gccctgggtg ccgttgatcg gaacgtggcg atagctggcg accacctcgt
    12901 cgagccccgc aacggtcagc cgcagggtgc cttcgaatcc ggtgctgacg ccgagcaggg
    12961 gccggatcgt ggtgtcggtg ccggggccca gggtgttgac gaggactgcg ccgccggtgt
    13021 cggcgagcaa ggtctggcga cgcgcctcgt ccggcgcggt gtcgtctccg ctgtcctcga
    13081 agaggagcag atcctggttg aggaaggcca ccagatagcc caccagctgc gtgccgggcg
    13141 tgccgtccgg tccgggagcg agcatctggc gggcgatgac cagctcgtcg cggccgccgc
    13201 cgcggaccgg cagcaggcgg atcagttcaa ggatgccggg ctcctgcagc gtgtcgagga
    13261 gctctggatg ggcgaatgcc tgtccggcct gtcccttccc gaccgcaccg agcagcgtgc
    13321 cggattccgc cgagatcacc tggaccgccc ggtagtcgtc cgggtaggcg cgcatcaggc
    13381 gttcgagtac ccgctcgatg tcgcgggccg cgtcgtcgtc gcggcgggcc atcaggtggc
    13441 cggtgatgac gcggttttcc gcgagggtga gcaggttgcc gcggtgggcc cgcaggcgcg
    13501 acaccaggcg tgcgcgctgg aagtcggccc cctgctcgag cagctggatc gaggtgccga
    13561 tgtagcgctg ccgttccccc tgcaggccaa ggcccggcac gccgaagtac cagccggcca
    13621 gaatggcgca gatcgccagc gtgaacagca gcgcaaagct gcggctgatc tgtgccgaaa
    13681 agctgggcag gcgggacggc gggcgcacga tccggtcagt cggtgtagtc gaactcggcg
    13741 agcctgaagc gacgcggctc ggcaaggacc tggtagagcc cgtcgtagcg cagggcgtcg
    13801 gcgatggcgt cccagcgtgc ttcggggggc agcttgccct gacccgcgag gaagtcgaac
    13861 tccttgcgca agggcggcga cgtgctgcgg cctggaatcg cgggcgccga ggggatgtcg
    13921 aggatctcct catgggtgat gtgggcgacc tgacggtcgg agatgggggg cgccttgccc
    13981 gacagcgtct gggcgtcgag gcgtgcccag gacaccgccc gatccaggtt cgcctgggcg
    14041 cggcgcatcc actccagcgc ccgcacaaag ccggcgatga ggtgccgggc ggcggcaggc
    14101 tgctgctcgg cgaagcgccg gtccagcacc aggaaatcgc tggtcaggcc gcgaaagacg
    14161 atccggttgg cgcggttggc cgcgagcgct gcgctcggtg ccggctccca ggcggcaaag
    14221 gcatcgatct cgccgcgctc cagggcctcg ggcatctcgc tcaccgccag cggtacgagg
    14281 cgcacctgac gctcggacag gccggccgag cgcaggccct gcagcagggt gtgatgggcg
    14341 ctggagcctt ccacgtagcc gatgcgtcgg cccgccagat cgctgacctg cgccacccgg
    14401 cgaccgacga tggcggtgga cgtctgcttc accagcccga cgatgaccgc ctggcctcgc
    14461 gcgcacgcca tcagagtcgg catgtccccc agcaggccgg cctccaggcg ccggtcggcg
    14521 agcaggggca cgatatcggc gccacgccag aaggcgtgga agcggggagg cgtgccgagg
    14581 gattcgaggg cctgccgcag gatacgatcg cggctcatca cggcgctgat gacgccggag
    14641 ggcacgccga ggggctggat gcccacgtcc acctccggcg atgcggggga caagccgacg
    14701 ggcggcgtgt cccgctcggc cagggcaggc atcgcgatcg acaggaccag caggcccagg
    14761 agcagtctcg gtcgcacgtc ggagtctccg atccgctgtg aggattgacg agcttgtcag
    14821 ttcagaccgt tttaacggcc gagcgcgacg ataacgttaa ttattccgaa aaaccagtgg
    14881 cattcttgtg gcctcggggc gcgtcttccg tgcggagggc gttggcctgg agtacagtcg
    14941 ccggcgctgg cggaagcgcc ctgccgcgcc gggccgtcgg ctgtgtgtgt gggacggcgg
    15001 ccgcgcgcct gcgcccccgc gtccgtgaat gcccgtcaag gagacccgaa tggacagtct
    15061 gagcgcagcc ctgcaacgcc tcttcggccg gcccggccag cctctgcccg atccgtggcc
    15121 ggagtccggc gcgctgctcg aaccggtgga cccggacggg cgcgcgtggg ccctcgtgat
    15181 cggtctgccc cgcccggccg gcggggcgat ggtgcgggcc ctgtgcgagg gggcgacggc
    15241 tctcgatctg cccgtgccgg cagtttccct ggcaggggac gcaggctttc acgtgtggtt
    15301 tgccctgacc gacccggtgc cggcggagcg cggccgggtc ttcctggagg ccttgtgccg
    15361 gcgctgcctg cccgagggcg cgtcccgagc gctgcggctg cccggcaccg tgggcctgac
    15421 gggcctgtcc ctgccgcccg cccgcgacgg cggacgctgg acggccttca tcgacccgtc
    15481 cctggccggc atgttcgatg gtgaaacctg gctggatatg gcgccatctc cagagcggca
    15541 ggcggagctg ctcgcgggtt tccgcgccat ccgcccggcc cagttcgacc gtgcgctggc
    15601 agaactcgat ccacccggcg ccgtggtgcc ttcgccgcgg ggcgccctgg ccgggcaggt
    15661 ggtggagccc cctgcgctga gcgggacggt gccgcccctc gggctgggtg tggggagcgg
    15721 cttcgtggac ccgcgcacct tcctgctcgc cgtgatgaac gatccgggcg ccagtgccga
    15781 tcaccgcatc gacgcggcca aggccctgct gccttatttc gaacgctgat ggcggtccgt
    15841 caccagtacc gcggccagtc cgcgtcccgc gccagattgt tatagaccag cgccacgccc
    15901 accagcagca gggcgccggc cagggtcggc agcagcagga aggaccagcc cggctggagc
    15961 agaaagacga tcaccggatt cgagcccgca ggcggatgga cgcagcgcag cgccatcatg
    16021 cccatgatgg ccagcgccac cgccagcgcc gcggcccacc agtgcggccc ggccagactc
    16081 aggcaggcca gaccgacggc cgagctcagg acgtggccca gcaccacgtt gcgtggctgg
    16141 gagaatggca cctccggaaa gccgaagacc agcacgcagc tggccccgaa cgagcccagt
    16201 acgagggcgg cggcagtgcg ttccgccagg cccgccacca cggcgatggc gaggaagccg
    16261 ccgagaccgg cgagcaggat ggcgcgcagc gggggcgccg gcggcagcgg cgcggcgtgg
    16321 ccgcggagac ggtgcaggaa agcgggcatg gcggagctcc ttgaggaggg tcaggtcggg
    16381 cgcggcgcgg gggtggccgg tccgctcgcc ggtcgggcga tgctacgggt gggccatgtc
    16441 cctgatctgc cggacctgaa ggagacagat ctgtctacat catcccatgg cgcatccgcc
    16501 ggtgcaagcc ggacgaatga ccggcgcccg caggtcgggc tttgcaagcc cgggtcggcg
    16561 cggtaaggtc tgagcatgac cgctgccccc ccctcccctg ccgaatccgc tgccccgatg
    16621 cattcccgct ccgagcgccg ggcccatctc gtggacacgg ccctgcgcct gttcgcccgc
    16681 ggcgggtacc acgccaccgg catcgatgcg gtgctggccg cgtccggcgt ggccaagaag
    16741 accctgtacc accattttcc gtccaaggac gatctgatcg tcgaggtgct gcgcgaacgg
    16801 gaccggcgct tccgggccag cctcgccgcc ttcgttgcgg cccgcgccac gccccgcgcg
    16861 cgcctggccg cggtgttcga atggcacgcc ctgtggtttg ccgagccgga tttcgccggc
    16921 tgcatgttcc tcaatgccgc ggcggagttc catgacgggt cggccgggat cctcgccgtc
    16981 gcccgcgaac acaagcaggc gctggaagcc tggctggccg cgctgctcac cgatctggtg
    17041 gaagcggagc gcgccgcccc cctggcggcc gacctcaacc tcttgctgga cggcgccatc
    17101 gggaccgccc tggtgagccg cgacccggcg gccgcagccc gcgcctgggc gctggccgag
    17161 cgcctgctgc ccgccaaatg aggagtttcc cgatgcgctc agcctgtctg gctgtgatcc
    17221 tgttggcggt ggccggcatc gcccacgcct ccgatgaagc ccgtctgccc ggcgacgccg
    17281 ccgccgtgcg tgccgccgat ctggaaaaac ccttcaaact gagcctcggc gagtaccgct
    17341 acggcggcaa cttcagcggc caggacgtga acctgcgcta tcgccgcggc gacagctcgg
    17401 cttggctggg cagctaccgt gaccgggact tcggcaccca gacccgcgcc ggcgcggaca
    17461 ccagctggca gcccttcgcc ggttcctcgc tggccctgca gccctccgtg cagctcgcct
    17521 ccggcggctt cgtcggcggc agcttcagcg tggaactggg cgaaccctgg ttcgtgcagg
    17581 cgggtatcgg gcgcaccaac acgcggccgt acttcaacct gaacttcgac cccaacgacg
    17641 cccagtccta cgccgtcggc tatcgggacg acagcggcca gacttactac ctgatgagcg
    17701 tccgcgacga ccggcttggc actcgccagc gtcacaccca cgccgttgcg cgctggccgg
    17761 cggggccgac gcggctcacg gtggacgtcc tgcacaagac tggcgacggc gacaacggcc
    17821 acgtggacgc ctggggcgcc accctgggcg tcgattacct ggactggttc gtgcgcctgg
    17881 tgcgggatcc gcaccagaac ttcggcgacg ccaacgcgac gcgcctgacc gtggggcgac
    17941 gtttctgaga cccgccgcgc tgaccaggtc acgcagatga agcctggggc gctgcctaca
    18001 gcgcgtcgag ccccaccagc gcccagccgt cccgcgtcag cgcgtcccgc aggaagcggg
    18061 cggtggcgac cgcgtccgga ccgtcgccat ggacgcacac gctgccgatg ggcgtcggcc
    18121 ggcgcacgcc gttcaccgag atcagggcct gctcggcgag catcgcgctg acgtgggcga
    18181 gggacgcccc ggcgccgtgg atcatcgcgt ccgggcggct gcgcggcacc aggctgccgt
    18241 cgtcctggta ggcccggtcg gcgaagatct ccgccaccac ccgcaacccg gcctcggcgc
    18301 cagccgtgaa cagcgccgaa caggccgggg cgaggaggat caggcccgcg tcgaagtcgc
    18361 ggatcgccgc gaccaccgtg cgcgccagcg ccgcatccgt gcaggccatg ttggacaggg
    18421 cgccgtgggg tttcacgtgg cccatcttca cgccttcggc ctgcgccatg ccggccagcg
    18481 ccgccatctg gtagatcagc accgcctcca gttcggccgg cggcagctgc atggggcggc
    18541 ggccgaagcc ctgcagatcc ggaaaggacg gatgggcgcc aacgcccacc ccggcggccc
    18601 gggcgtggcg cagggtcgtg cgcatcacca gcggatcgcc ggcgtgaaag ccgcaggcga
    18661 tgttggcgct gttcaccgtg gcgagcagct cggcatcgct gcccatcgtc caggcgccga
    18721 agctttcgcc cagatcggcg ttgagattga tgcggcggtt catggtcggt gtctttcctc
    18781 cgggttcgtc ttcaccgcgc gtgcggtggc gaggggcgat gcgcttccgg gccgcaacgg
    18841 cccgtgtgtc agtcctcgtc gccgcgcagc acaccgccga ccaggttggc ggcgtagagc
    18901 gccgttgcat cgaagccgcc ggcgggtggt agcgcggaga gggcggctcg ccaggcggcg
    18961 aaaagccggg cctgttcctg ccgggcgcgg gcggctgcgg cggcgtccac ggcgctgaag
    19021 ctgagcgtgt cgccgggccg caggtgaccc aggcggggca ggtcggcggc gatgacggtg
    19081 gcaatcttgg ggtatccgcc ggtggtctgg caatcggcgc gcagcaggat gggctggccg
    19141 tccgccggca cctggatcgc gccgggcgtc acgccgtcgg tgacgatctc ggcgccccgc
    19201 gcgctgtggg tcaggcgcgg gccggccagg cgcgcgccca tgcggtcggc ggcgggcgtg
    19261 acggtccacg gcccggccag gaaggcagcg aagcaggcct cgtcgacatg ctcgcgctgg
    19321 ggcccggcga tcacccgcag cggaccggca gggaggcgcc acggccgacc gacccgttcc
    19381 ggcccgtccg gggccgcacc gcaaggcagg cggtcgccag cctggatcgg gcgtccggca
    19441 atgcccccca gcccggcgcg ggcgtaggtg gcgcggctgc ccagcacgac cggtaccgtc
    19501 atgccgccgg acaccgccag cacggcgacg ccggcttcca cgcgccccac cgcgaggatg
    19561 tcgccggggg tgagggtggc gctgcgccag ggcggcaggg gctcggcgct gccgtcgccg
    19621 cgcagcacgc ggctcgcccc ggcgccggcg agggcgaccc gtaccggacc gtctgcggcc
    19681 tgcaggcgcg gtccgtggag caggatctcc agcgctgcgg cgtcagccgg cgcgccggcc
    19741 agggcgttgg cggcggcgag cagcagtggg tccagggcgc cggagacggg cacgcccagg
    19801 gcgcggtagc cgacgcggcc gcgatcctgc acgctgacgc cgaggccgcc gtcgaggacg
    19861 tggaggtggg tcatggggcg gcccgcaggc gatccgggtc agcgcgtccg gcggtgaagt
    19921 cggcggccag cgtgtcgaag tcggagcgcg tcacggcctg ccagcgcacc cggtcacccg
    19981 gcgccagcaa ggccggtcgc gcgctgcggc ggacgtcgaa gcagggtacc gggcagcgtc
    20041 ccagcaggcg ccagccgccg gggctggtcc atggatagac ggcgcacagg gcgtccgcca
    20101 cggccaggga gccggctggg acggcggtac gcggtgtggc caggcggggc tgtgccagct
    20161 ccgccggcag gccgcccagg taggggaaac cgggcaggaa gccgagcatc cacacgctga
    20221 attccgcgcc ggtgaggcgg gcgatcacgc cggcgcggtc cagcccgcag cgtgcggcga
    20281 ggtcgtccag atcggggccg aaatcctcgt cgaagcacac cggcagcgtc caggtcgcgc
    20341 cgttcagcgg cacggcgtgg gtctggcggg cgaggctcag caggtgcgcg gcgagggcat
    20401 ctggatcggc gttgtccggg tcgaagaaca ctgtcaccga cgcgaaggcc ggcaaccatt
    20461 cgatcacgcc gggcagcgcg cccgcgtccg ccgccgcggc cagtccggcg gcgaagcccc
    20521 gcacgcgggc gtgcaggtcc ggcgcgatgg tcgtgccgaa ttccaccgtg aaggcggtgt
    20581 cgccgagggg cagcaggcgg ggggcgttgg gcgtggcgga catgggcgcg ggggctgtgc
    20641 gcggcatctt acgcgccgtc tgcggggcgt tccaggccgg tgcgacgccc tcgcgtggat
    20701 gaagatgctg gcgccgcggg tccgggaaga tcgcctccgt cgggcaggac ggcgcctgtc
    20761 gcgaaagcgg atggacgaga cccgggtttc gtctgtgcca atgagcaccc ggcacagcgg
    20821 gtgtgagcct gccggtcgca cccgccgggc gggttgccgc gtaccggcgc gcggcgcggc
    20881 gcttactgcc cgagggcgcc gaaggccttc ttcgccagcc cggtggcggt gcccagcgga
    20941 tccttgcgga tcgccttctc ttcctcggcg atcatcagga acagcccgtc cagggccttg
    21001 cgggtcacgt agttctccac cttcaggtcg tcccccttgc ccaggccgaa gctgccggcc
    21061 ttgcccgcca gggcgttgta ctgctgggac agggccagct tgtcggtggt ggccttgacg
    21121 gtgggcagga aggactcgaa gagctgggcc gaggtcttct ccttgaagta ctgggtcgcc
    21181 gcgtcgtcgc caccgccaag gatcttcttc gcgtcgtcca cgctcatctt cttcaccgcg
    21241 tccaccaaca gcaccttggc cttgggcacg gcctgctcgg cggcgtggtt catggtggcc
    21301 acgaggctgt ccagatcctt gccgcggccc atggtgcgca gcacgccttc caccggcgcg
    21361 aggctctccg gcaggccgat cttcactttc gggttgttga ggaaacctcc gtcggtgccc
    21421 agcagtccaa cggccttgga tgcgccctgg ctcagagcct ctcgcaggcc gccggaagcg
    21481 tccttgtcgc tcaggtccgc caggccgaag gcccacgcct gggtgcagat gacaagcagc
    21541 agcaaagctt tcagaatccc acgcagcgta cgcatcgccc aatctcctga ccggtcttaa
    21601 atcgaatgtt gcccaaggct atcacgcgct tcattgccgc cctgctgttt cttgcacttc
    21661 ttgacggatg cagcgccccc gccgcgccgg acgtgagctt caccaccctc gacggccaga
    21721 ccacccgcct gtcggcgctg cgcggcaagg tggtgctggt gaatttctgg gccaccacct
    21781 gcaccacctg cgtgggcgag atgcccaagc tggtggacac ctaccggcgt ttcgccccca
    21841 ggggcttcga gaccgtggcc atcgccatga gctacgaccc gcccgagtac gtgcgcaact
    21901 acaccgccaa ggccggcctg cctttcaagg tggcgctgga tgcccgcggc gaggccgccc
    21961 gcggcttcga ggaggtgcgc ctgacgccca ccaccttcct cctcgacaag cagggccggg
    22021 tggtccagaa atacctgggc gagccggatt tccccaagct ccacgccctc ctcgaaaaac
    22081 tcctcgccga tccggcgtga agcgccccgt cctgagtgtc gccctgctgg cggcggccgg
    22141 tctggcggcc cttgtgctgt ggccccgcgg ccgcgaggtg gacggcgtcg ccgtgcgccg
    22201 cgcgcctctc acccagtccg tcgttgccac cggacgcatt gccaccccgg cgcgcatcac
    22261 cgtcggtgcg ccgctggccg ccaccgtgct ggccatcagc gtgcgtgaag gcgactccgt
    22321 caaacccggc caggtgctgg cccgcctgca ggccgacgac gccgatgccc tggtgcgtca
    22381 ggccgccgcc gctctggccg aagccgaagc ccgcctcagc cagatcgcca ccgtgggcga
    22441 gccggtggcc gcccaggccc tggctcaggc ggaggccaac ctgcacgtgg cccggcgcga
    22501 ggccgagcgc tcgcgccagc tggtcgccca gggcttctac tcccagtccc gggtggatga
    22561 cgccctgcgc atcgagacca acgcccgcaa cgccctggac accacccgcg cccagctgcg
    22621 cgcccagcag cagggcggca ccgagcggga ggcagcgcgc gcccgcgtgg accaggcccg
    22681 cgcccagctt gccaacgccc gtgcccgcgc cgccttgctc accctcaccg ccccatcggc
    22741 gggcacggtg ctcagccgca aggccgagcc cggcgacgtg accaccgccg gcaaggtgct
    22801 gctggagctc gccgaggccg gcgagacccg cgtctatgcc accgtggacg agaagaacct
    22861 gcgcctcctg gccctcggcc agaaggcccg cggcgtcgcc gacgcctttc caggcacgcc
    22921 cttcgaggcc gaactgtact acctcgcccc ggccgtcgat ccccagcgcg gcaccgtcga
    22981 gatccgcttc cgcgtacccg cgccgccggc cttcctgcgc ccggacatga ccatctccgt
    23041 cgagaccgtc accggccacc gggacgccac cctggtcctg cccagcgagg cgctgcgtga
    23101 agccgacggt ggcgcaccct gggtgctcgt cgcccgcgac ggggtggccg agcgcgtgcc
    23161 ggtgcagacc ggcctcaccg gcatcggcca ggtcgagatc ctgcacgggc tggtcgaggg
    23221 cgacgttgcc atcctgcccg cctccggcgc cctggacggc gaccgggtgc gcctgcgcga
    23281 gatgaagaag ccccgcaaca ccctgcaggt gccgaccggg atggcgcgat gagggcggct
    23341 cggagagggg ctgagcgagg cggggccgcg cgggctggaa cggcgcgagc tgtcggagag
    23401 ccggtttccg cggcgacctc cacccatgga caaagccaag cctgccccga tacgggcgcg
    23461 gttcagcggg cgggcgtccg cggtaccgac tccgtccccg gtcgggaaaa tccccgctgt
    23521 gcatttttaa atggcccggc cggatttcgt cgcctctttt tgaggcagat aaatgcccgc
    23581 agcccattta aaaatccggt gaccggattt ttctgcctca aaaagaggca agaaaatccc
    23641 tttggcgcat tatttaaggg tcgtcacccc tttaaaaatc cgaccggtac atttttaaat
    23701 gtctccgccg gattaaataa tgcccggcgc ccatttaaaa atggttgccg ggtattttcg
    23761 tcggcagcgg cgggggcggg ctggcggtcg tccgccgccc gcgtcacccc gcgttccgcc
    23821 gggtccgcca ccccggctcc cgccggcgcc gtccgatgac cctccgcctc ccctttgcgc
    23881 ccctcgtggc cctgcgtttc ctgcgggaag ggcgcatgca gaccctgctg atcctcgccg
    23941 gcaccaccgg cggcgtggcg gtgatcgtct tcctgaccca gctcatcacc cagctccagg
    24001 cccagatcgt ggatcgggtg ctgggcagcc aggcccacgt ggtgatccgt ccgctggagg
    24061 aggccaaccg tcgcgccttc gcgcccgacg gcatggccac cgtcgttgag ccgcgcgccc
    24121 agcgcctgcg ctccatcgac cagtgggaga gcgttgcccg cctggccgcc gggctgcccg
    24181 gcgtcacggc cgtgtcaccg gtggccaccg gcccggcctt tgcgacgcgc ggccaggcca
    24241 acaagtccat cgccctgatg ggcgtggacg cggaccgcta ccggcgcatc gtgcgcatgg
    24301 aggaatacat gaaggccggt cgctttgcgg tggacggcac ccgcgcggtg atcggcgccg
    24361 acctggccac cgacctgggc ctccgcctgg aggacaagct gcgggtgatg cttcccaacg
    24421 gtcgcgacga gctgctccag gtgggcggca tcttcgacat cggcaacaag gacctcaacc
    24481 ggcgctgggt cttcgtcacc ctgcccctcg cccagagcct gctggatctg cccggcggcg
    24541 tctccaacct ggacctgacg gtggccgaca tcttcgaggc ccgccccatc gccgagcgcc
    24601 tgcgcggcgc caccggcctg accgtggaca gctggatgga gaccaacgcc cagctcctcg
    24661 ccgccctgcg caaccagacc gtctccaaca acctgatccg cagcttcgtg gtcctcatcg
    24721 ttgccctggg tatcgccagc gtgctggtgg tgagcgtggt gcagaagcag aaggagatcg
    24781 gcatcctgcg cgccatggga gcgcgccgcg ggatgatcct gcaggtcttc ctgatccagg
    24841 gcgcggtggt gggcgccgtt ggctcggtgc tcggcagcgc cctcgccttc acgctgctgc
    24901 gggtgttttc cagcatctac cgcaacgctg acggctcgcc cctgttctcg ccccagctgg
    24961 agctgcgctt catcgtcatg gcggccctgg tggcgaccgc cgtcgggctg ctctccgccc
    25021 tcatcccggc ccgccgcgcg gcgcgtatgg acccggtgca ggcgatccgc gcatgagcgc
    25081 cctgcgcccc gccgccgccg gcccggtgct ggaactggcg ggcatcacca agacctacaa
    25141 ccccggcacg ccggtggcca ccgaggtgct ccacggcatc gacctcagcc ttgccgaggg
    25201 cgaattcacg gcgctcgtcg gcccctccgg ctccggcaag agcaccctcc tcaacctcat
    25261 cggcctgctg gaacggccca gcgccggccg cctcctcatc gccgggcgtg acaccggcca
    25321 gctcgacgag gccgcgctca ccgccctgcg cgggcgcagc atcggcttcg tgttccagtt
    25381 ccaccacctg ctgccggcct tcaccgccca cgagaacgtg atgatgccgg ccctcattgc
    25441 ccatggcgtc gccgacccgc agatcgaggc cgacgccctg gccctgctgg acgccgtcgg
    25501 cctcaaggcc cacgcccaca agaagcccgc ccagctctcc ggcggccagc agcagcgcgt
    25561 cgccatcgcc cgcgccctgg ccctgcgccc gcgcctgatc ctcgccgacg aacccaccgg
    25621 caacctggac acgcgcaccg ccgacgacat cttcgccctg ctccgggaat tcaatcacca
    25681 gacgcgctgc gcctgcctga tcgtgaccca tgacccacgc ctcgccgccc gctgcgaccg
    25741 cacggtgacg ctggtggatg ggcggctggt gatctgagct cattccgccg gcgtgccgcc
    25801 tccaggtggc cgggcgaatc cgcgcgggag gcgcccgcca gtgggacgcg gcgagggcat
    25861 cccgtggctc agcggcacgc caccggcttg cccgacgggt cgctgcatcc gccgctgtga
    25921 ctgacgcctt cgccgcggga gtaggccgtc gtgcccgtgt aacgggtgcc gtcatcgcgg
    25981 gtggcggcgc tcgagtggct cacgctgtcc tggtcgtagt gggtgctgcc cgcgtagctt
    26041 ccgccggcgc tgccctgggc ggtggtgctg cggtcgccat acagcgtgcc gtcggcggca
    26101 cggctgacgc tgccctggct gtcgaggctg ccccgcgcgc tccgggcagc gaagcggctg
    26161 tcacgggtga gcgcgccgtc ggcttcgcgg gtgaggcggc tggcgtgagc cgcgctcgca
    26221 ccctccgcgc cgcggaatac ccccgcgcgg gtggaactga tctggccggt gccatcgctt
    26281 acggaacgcc tgccgtgggc gaacgtggtg ccgtgggggc cgatcccggc atcgacgtgg
    26341 cgggcggtag tgccgccagc ggggttggga tgcgcgaggc gctcccgcgc atgggcggcg
    26401 gcagggagga cggcgagggg cgcggaacac agggtggcca ggatgaagac aaacgggcgg
    26461 cgcatggcga ggtcctttcc ggtggcggtg gcggggatgg ggcgcaggcc gcagtctagt
    26521 cctggacgcg cggtgggcat gtgtcgcggg gacggcgaag tgtgagggga tgtacggcct
    26581 ccgccggcca cgggcgcgga ggcgggagcg gaacgatggg aacaaaaacc ggtatcctcc
    26641 gcccatcccc cacttccccc cggagcatca tcgtgtcgga cacccgcgag ctggccaagg
    26701 aagcacttgg caccttcgaa tcggccatca tgggcatcgc cggaacggcg ccggccttca
    26761 gcgtggcggt gacgacggcg gcgatcgtgg cctcggtagg cgtgctgtcg gtggggagca
    26821 tcctgtactg cggcctgatc atgttcggga tcatgctggc tttcgtgcat ctgtccaaga
    26881 tctcggccca tgccggggcg gcctatgcgt gggtggggga ggtcttcggg ccggtgtggg
    26941 gcttcttcac cggctggggg gtgctggtcg cgtcgacctt cttcatggtg tcggcgacga
    27001 tccccgcggc cacttccacg ctggtgatcc tgatgccgga caaggtggag gacaccctgt
    27061 gggtcacgtc caccgcggcc ctgtggctca cggcggtgac cgcggtggtg accaagggca
    27121 tcaagcacgc gagctacgcc caggtgatcc tgaccgtcat cgagacggtg gtggtgctgg
    27181 ccctgatcgt ggcggccttt ctggaatacg gcagccggcc ggcccatccg ccgtcgatga
    27241 tctggttctc gcccttcgcc ttcacgccgc agatcttcgc caccggcgcc ctgacggcaa
    27301 tcttcttcta ctggggctgg gacgtgacca tgaacctcag cgaggagagc aagcaggcgg
    27361 ccggcgggca cacggcggcc gggcaggggg cgttctgggc gatggtcaac ctgatcctgt
    27421 tcttcgtgat catgatgatc gtggtgctca tcgtgctcaa cgacgcggag atcgccgagg
    27481 ccaacaccaa cgtgctgtac gccatcgcca acaagctctt cgcgccgccc tggaactatc
    27541 tggcggtgct gtccaccatc ctcagcacgg tgggcaccat cgagacccag atcctccagt
    27601 tcagccgcag catgtttgcc atggcccgcg acgagatgct gcatccgcgc tacgcccgca
    27661 tccacccgga gtggcagacg ccgtgggtgg ccaccgtggt gatctggggc ctgggggtgg
    27721 tgctgctgtt cgcgtcgtcg gcgctgccct cggtcaaggt catcctgcag agctcgatcc
    27781 tcgccatcgg tttccagatc tgcttctaca tgagcctggc gggctttgcc tgcgcctggc
    27841 attaccgggc caagctgggc gaggggctga aggcggcgac gagctatgtg ctgtggcccc
    27901 tgggcggggc gctgttcatg gtgttcatcg cggtgtacag cgtgccgacc ttcgatctca
    27961 tcaccaacgt gtcgggcatc ggcgggttgc tgatcggggc gattccgctg atcctcaacc
    28021 gcaagcgggt gatggcgggc gcctgagcgc gggcggcgct gcggcgcggc atcgccgaaa
    28081 cttggggact cgcgcgaccg gcatccgggc ggccttcacg cgtctgccag cgtaccgccc
    28141 gctgtgtcgg ggaggttcgg ctcagccagc ggcagcaggc gctgcaaggt ctgcacgtag
    28201 gtgcgcaggg cctcgctgcc ctcggcggtg agggcgtagc gggtctgggg ccggccgggg
    28261 ccggtgtcgc gggcgaccga cacgtagccg gccgccacca gcttcttgag atgggattcc
    28321 agattgccgt cggagacctg gagcccccgt ttcagttcgc tgaagctggc ctcgccagcg
    28381 ccggccagaa aggcggcaag ctgggtgcgc aggggctggt gcagcagggg gtccagggtt
    28441 tccatcgtgg cggctgtcag ggcttgaggt tgaattctgc ccgcaatacg cccggcgcgc
    28501 ggctgtcgcc ttcgcccggc acgacgaact ggaagtggcc gccgatcagg tcggtggcgc
    28561 ggcaccaggt cttgccgtcc aggctggccc actgcttgta ggtatagatg tccttgcgga
    28621 tgctcacctg cagggtgctg cgggtgtcct gttccatcag gctgccatgg atgcgggctt
    28681 ccaccggcag gggggcgccc gccgggtagt ggaggatgta gggctggccg gcaggggcgg
    28741 cgtcgccgaa acggacgacg ggcagctgtg ccagttcggc atcggagggg cggatcaggc
    28801 tggcgcagcc gggcagcacg aggctgacca gggcgagggc ggcaaggcgg tgcatggacg
    28861 actcctaggg agggggaaaa tcagtggaag gggtggccgt ggaagtccac caccagggcg
    28921 gtatcgacgc tgggcccgcg gccggcttcg aggcccgcgc ggatccacgg aatggagatc
    28981 cagcgggtct cccgggcgag ctgccagggg ccgccggggg cacgcacgtc gccagtgagg
    29041 gcgccgtccc gcagcagcag ttccacgggg cggtcgagca ccaggggcag cacggcgtcg
    29101 gggtcgggac ggaacagatc gccggacacc tgtacccgca ccggcacgcg ggtgccggcc
    29161 gggagctgga cgatgcgttc tccggtcatc gtgccgcccg ccatgaactc ggccagggac
    29221 atcaccggca gctccggggg caggctcgtc gacgcgtagc ggtgagccgc caggggggcg
    29281 gccaggacga gcaccgtcca gccggcggac tgggccaagc gcacgccgaa gctgcgggtc
    29341 cgcccgtggt ccagcagcgc tgacaggagc ggcaggccga gcccgaacac ggcgatggcg
    29401 atccacttca tggtctcgaa gggcagccgc gccaccaggc cggccacgcc gatcaggatg
    29461 atggtgccac ccgcccactc cagcacttct tcggagaaca ggccatgcac gtacagggcg
    29521 aggccgatga ggaccagcca cacggtgcac agcatgtagg cgccgccgta gaagaaggtg
    29581 gcgaaggtga acagggtgcc aacgcccatc agcagccaca gcagcttgac cacctgacga
    29641 tggatgaagg accaggtctc gtcgcgggcc tgcttgaccc gccgcgtcag atgccagtcc
    29701 gccactccga cgccgcccag cacggtcgag atgaacagca gccaggcggt ggcccgccgg
    29761 gacgcgtcgg ggatctgctc ggcggtgagg acggtgtcgc tgcaggcgaa cagcagcgca
    29821 ccggcgaggc cccacaggat caggctgtgg cgctccatgc gcaggttgcg ctggccgcgc
    29881 aggagcatgg actggatggc gtcgagctga cgggcgatgg ggtcggccat gggcgggcgt
    29941 cctattaact ctgtgacgca gagcttataa ctctgaatcg cagagtgtca acgcgtgccc
    30001 catggaatat tcgcagggcg agaccgactc cggtccggcg cccaacgccc gcggcgccaa
    30061 accttgccgc ggccgggcgt ggcgcggcgg gggcgcatcg atgggccgag gcacggcagg
    30121 cctcccgcgg ccggcggcgg tcgtgacgaa agatgccccg cgcaccgccg gtgacgggca
    30181 ttccgtggtc cgcgaatgtt cggggccgtc ccggaatcgg gggacggcgt ggggccatgc
    30241 ggcggctgcg accccgtccg cggggtccgg gcggtatcat ccgccctccc cttccgcgtg
    30301 cccgccgcca tgtccgatac cccgccgcag cgcttctccg acgccgacat cgccgccgtc
    30361 taccgggcca tcgccgagcg ccgggacatg cgccatttcc gcccggaccc ggtggcgccg
    30421 gcgctgctgg agcgcctgct gtgggctgcc caccatgcgc ccagcgtggg ctacatgcag
    30481 ccctggcgct tcatccgcat caccgaccgg gccctgcgcg aacgcctgca cgccctggtg
    30541 gaggtggagc gttgcgccac cgcgcgggcc ctgggcgagc gggaagatga attcatgcgc
    30601 ctcaaggtgg aaggcgtgcg ggaggcgggc gaggtgatcg tcacggcgct ggcggacggg
    30661 cgcgagcgcc acatcttcgg ccgtcgcacc ttgcccgaga tggacctggc ctcggtggcc
    30721 tgcgccatcc agaacatgtg gctggcggcc cgcgccgaag gcctgggcct gggctgggtg
    30781 tcgatcttcg atccggccga ggtggcgacc ctgctgggtt tgccggaagg cgcccgggcc
    30841 gtggccatcc tgtgcatcgg ccacgtggag gccttctacc cgcggccgat gctggagatg
    30901 gagcagtggg ccgagcgtgc gccgctggcc accgtggtct acgaaaacgc ctggggccgg
    30961 acagggacgg ggccggcgga cccggcctga ggccgggcgc gaaggccccc gcccgcggac
    31021 cgggcgcgtt ccgcgtcagc ggttcacatc caccaccacc cgcccgcgca cctgtccggc
    31081 gagcagcgcc gcggcggtgg gaatcgcttc ggcgagtccg atttcgtggg taatgaggtc
    31141 gagcttgctc atgtccaggt cgcgggcgag gcggttccac gcctcgatgc gctcggcgcg
    31201 ggggcacatc acgctgtcca cgccggccag ggtcacgccg cgcaggatga agggcgcgac
    31261 ggtggcgggc aggtccatgc cctgggccag cccgcaggcg gccacggtgc cccggtagcg
    31321 catgctggcg cagacgttgg cgagggtgtg gctgccgacg gtgtcgaccg cgccggccca
    31381 gcgttccttg gccagggggc ggccgggggc ggagaacggg gcccggtcga tgacttcggc
    31441 ggcgccgagg gacttgagga agccggcttc gtccagtcgg ccggtggagg ccaccacggt
    31501 gtagccgagc ttggccagca gggcgatggc cacgctgccc acgccgccgt tggcaccggt
    31561 gaccagtact tcgccctggg cgggcgtgac gccgtgacgt tccagcgcca tcacgcacag
    31621 catggcggtg tagccggcgg tgccgatggc catggcctgg cgcggcgtga aggcccccgg
    31681 cagcgggatc agccagtcgc cccgcaggcg cgcccgctgg gccaggccgc cccagtggcc
    31741 ttcccccacg ccccagccgt tgagcaccac ggcgtcgccc acggcgatgc cgggatggct
    31801 gctggcctcc accgtgcctg ccaggtcgat gccggccacc agcggaaact tgcgcaccac
    31861 cgggcctttg ccggtgatgg ccaggccgtc cttgtagttg aggcaggaat acgccacgcg
    31921 gacggtgacg tctccttcgg gcaatgcgga atcgtccagc tcgcgcacgc ccgcgcggta
    31981 gccggcttcg tccttttcga tgaggatggc cttgaacatg tctgtctccc ggtgaatggt
    32041 gttgtcaggg tggcacgcgc cacgcagcaa cgatagcccg ttgcggcagg cagggccagt
    32101 ggcccgggcg gcgtcgcgcc tcagggcgtc cacaggggcg cgccggccgc gtccaggtgg
    32161 gtgaggtaga ggcgcacgtc gagctccagc tgcgcataat ccggcgccat gtggcagcac
    32221 agctggtaga aggccttgtc gtgttcgggt tcgcgcaggt gggcgagctc atgcgccgcg
    32281 atcatgtgca ggaagggctc cggggccacg cggaacaggg tggcgatgcg gatctcccgt
    32341 tttgtccgga gccgaccgcc gtgcacgcgg gtcacgcggg tgtgggtgcc gagggcctgg
    32401 tggacggtct gcagcgtgcc gtcgtagcgc accttggcga ggggcggcgc ggtgcgcagg
    32461 aagcggttgc ggagcgcggc cacatagtcg tagagcgcgg tatcgctgcg caggccgtgg
    32521 gtgtccgggt agcgctgctg cagccagggg ccgaggcggc cggtggcgag ggcctgggcg
    32581 gcctgggtgc ggacggcttc ggggtagccc agcaggtagg gcaagggggg agatgacatg
    32641 gggcggcaga cggcggcggg gcggctcatg atgccgcccc gaccgggttt ccgccagccc
    32701 ggtggccgtc agcccttggc ggcgagcagg gcctgaagcg gcgggaggca ttcctcgccg
    32761 accggcgcga aggcgctgaa acgcaggtcg tcgagcacct tcctgccggc cggatcctcg
    32821 tgcatgccgc acaggatggc ctggatgtcg gctcgcttgt cggcccattc gggcgatacc
    32881 atgaaacagt ggaaggccac gccgtcggtg ctctcgccca gcacccgcag gcttgcgcgg
    32941 gtcgccgggc tcaggccggc ccaggtctcg ttgaagacga tgcccaggtc ggcgcggccg
    33001 tcgatgacgg ccttggcggc gttgaggtgg ttgccggcga actcgaagcg cgccgcggcc
    33061 gggtcgatgc ccagcgtgcg cagcacctgc aggcccagat cgtggatgat cagcttgtcg
    33121 gtggcactgg cgacggtggg ggcgtccggc accgcgcctt cggtccgggc gacgatgacg
    33181 gcctcgtcca ccacacccgc cggccgcgcg accggcacga agcccttgtc gcgggcgaag
    33241 cacagggcgc tgtaggggtt ggcatagacc acgtggaaac gggcgtcgag cacgcgctgg
    33301 cgttcgacga ggaagttgtc ctggggctcg aagtggatgc cgatgcccag cttgcgctgg
    33361 aggtaggtgt tgagggtgaa ccaaccgagc aggtttttgg cggtatcgtg ggggcagacg
    33421 ctcaactgga attcgctggc catgggtctc tcgtgggatt gggggtgaaa cctcgacggg
    33481 ccggaccggg ccggcggcat cgaatcattt tctgggacga taaaagtgga atataacaat
    33541 tccaatcgca ttcatgccac gcattctgtc ggtgcccgtg acatccgtac ggcgggcggc
    33601 cccggtttgt ccgggcgcgg cgcgggcggg cgcgcgcctt tcgcctatct gacgcggctg
    33661 ccgcccggca aggcggtgct gtggtgttac ctgatctggt acctgtccac ggtggcccac
    33721 cacttcgacc cctcgcccaa gctgtggctc aacgccctgg gcatcagcgg ggtgatcggc
    33781 attgccctga tgctcagcgt ggccggcaca acccgtccgg accgttggca gacggcgcgg
    33841 ctgttcatga tgcccttcgg cgtgtcgagc ttttcggcgt tgatcaaggg gcagggcttc
    33901 gtgctgatct tcccgccgac ctgggccgaa tgcgcggtca gcgccggggc ctgcgccttg
    33961 ttcctcgcgg tcgtggccgc cgcaaaggcg ctggtgcggc agccgggcac gacgcctcag
    34021 tagccgccga tgcggcgcac gagggcgtat tcgcaggact tgaacaccag gtagaagacc
    34081 atgctcagca cggtgctgag cagcaggtcg agcagttgac cgagggcgcc atccatggcc
    34141 gccatgctgc cgtgttccag gaacagggcc aggttggcct tgagcagggt caccagattc
    34201 agcgacgcga tgccgaaggc gacgaaggac agcgcgagca ccacgaaggt gagccacgcg
    34261 cgggcgaaga cgaattgacg aacggtcttg agcatggccg gctcctgggt gtggggtctg
    34321 atgtgcacca gaccgggccg gaccgccgcg ccttacatcc agtccaccag cgacacgccg
    34381 aaaccgatca cgttctggcg gtggttgtag tcgatcaggg tctcgccgta gccgctgaac
    34441 acctgcaggt agcctttcag attgcgatgg atcgggaagc tccagtccac gccggccgag
    34501 ccgcgcccgg cggtgggatt gccgcgcagg ttgaggccga gggtctgctg actgaagcgc
    34561 cacacggcct gcacgtcgcc gtggccgagg tagtgggtga tgtcggcgtt gtcgtcgttg
    34621 cccgcgtttt catggaggcg cacccagggg cgcacgatca gggaaaaatc gcccctttcc
    34681 acgccggcct gggccatgac ccggttccag ctgcgggaca gatcgcccgc acggccgttg
    34741 gactggtggt tgaggccgat ggagagcagg cgcagacgga aaccggcgcc caggtcccgg
    34801 ttggtacgga acaccgccat cagctcgggc tcgtagtcgg tctcgcggaa gggcgaggag
    34861 ttgcccttgt tgtagacctg ccagctggat tgctgggtat agcccagcca aaggtcgcca
    34921 tgccggccga acaggccttc ccacagcttg gtcttgaagc tgagctgata cttcgcctcc
    34981 accgaatcca gcgtgccgct ggtggcagcc gtgcccaggc gcggcgaggt gggggtgggg
    35041 ttgcgggcgt cgctccagcg caccggcagg atgtagctgg gcttgtaggg gcggaacagg
    35101 aaggtgccgt gcttgtc
//
LOCUS       DAOQMM010000027        34605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_323_length_34605_cov_49.746049, whole genome shotgun sequence.
ACCESSION   DAOQMM010000027 DAOQMM010000000
VERSION     DAOQMM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 34605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34605
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_34605_cov_49.746049"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLH85_06700"
     CDS             complement(<1..538)
                     /locus_tag="PLH85_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNB64286.1"
                     /translation="MTLRSTLAAGVAALVLGVPVARAADVAQFAPCVAGLKAGAVARG
                     VSPAGYERLTAGLEPDLKVLDFLDYQPEFRTPIWDYLSGLVDDERVADGQAMLKQWGD
                     TLAAVAARYHVDPATVVAVWGVESNFGRNFGTRPLLTSLSTLSCYGRRQAYFRGEFYD
                     ALKIVDGGDVKAEQLTGSW"
     gene            complement(538..1068)
                     /locus_tag="PLH85_06705"
     CDS             complement(538..1068)
                     /locus_tag="PLH85_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HNB64287.1"
                     /translation="MQHHRSARLPVLFLLSCAALALGACSSAPKKDLTYRSERFNTNS
                     PYEVAVDVAPTAACDAGRRALLSQGYVIEDAKPDAVKARKFFQPERNVHVQINFVLSC
                     LADGDGTEVFANAREVRDELKASSSSAGVSVAGIGSLNLPLGGNGESLVKVGEETITD
                     SDFYGRFFALMQTYIR"
     gene            complement(1155..3887)
                     /gene="polA"
                     /locus_tag="PLH85_06710"
     CDS             complement(1155..3887)
                     /gene="polA"
                     /locus_tag="PLH85_06710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNB64288.1"
                     /translation="MPTLLLVDGSSYLYRAFHALPDLRNSAGEPTGAVRGVLAMLRRL
                     ESDYKADYRACVFDAKGKTFRDDWFPAYKAQRPPMPEDLRAQIEPLHAAVRAEGWPLL
                     MEDGVEADDVIGTLTRQAAEAGWEVVISTGDKDLTQLVGPGVRWVNTMSEEVLDAAGV
                     EAKFGVPPGRIVDYLALIGDTVDNVPGVEKCGPKTAVKWLSQYGDLDTLVAHAAEVGG
                     KVGENLRKHLDFLPLGRKLVTVVTDLPLPVGPADLAVRPADRAALAELYRRMEFKSWL
                     KELESAPPAGASETPPAGDAPPPSAEPAAPGAHRQGYATLLDWDAFDAWLAQIQAAEL
                     TAFDTETTGLDPLVATLVGMSFATVTGQAAYLPLAHTYADAPAQLPLAEVLARLKPWL
                     ESDAHRKLGQNLKYDAHILANHGIRLAGIAEDTLLESYVLESDKSHDMDSLASRHLGL
                     KTLTYAEVCGKGAKQIGFGEVDLARATEYAAEDADITLRLHHALSAQLAAEPALAALY
                     RDVELPTARVLFEMERTGVLIDDFLLAQHSEELGRRLHALELDAHALAGQPFNLGSPK
                     QLGEILFTKLGLPVVKKTATGQPSTDEDVLTQLAEDYPLPKLLLEHRSLAKLKNTYTD
                     KLPLMVNPRTGRVHTSFSQAVAVTGRLASTEPNLQNIPIRTPEGRRIRAAFIAPAGHR
                     IVSADYSQIELRIMAHLSGDAGLLNAFAHGEDVHRATAAEIFGITPMEVGSEQRRTAK
                     VINFGLIYGMSAHGLARNLGIERAAAATYIDRYFARYPGVAAYMERTRTEARTQGYVE
                     TVFGRRLYLPEIRAQQVGRRQAAERAAINAPMQGTAADLIKKAMIAVSAWLKTAGVRA
                     RLILQVHDELVLEVPDDEVALIRERLPQLMAGVAQLSVPLVAEVGVGANWDEAH"
     gene            3944..4669
                     /locus_tag="PLH85_06715"
     CDS             3944..4669
                     /locus_tag="PLH85_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB64289.1"
                     /translation="MTAKDKLPKMAPGSESQHFTARESWRIFGIMAEFVEATERLKPI
                     RPAVSIFGSARVKPDHPYYAQTERIARLLSDAGFSVISGGGPGIMEAANKGAYFGKSP
                     SVGLNIQLPMEQQANPYQDISQTFQHFFARKFMFVKFASAYVVMPGGFGTLDELLEAM
                     TLIQTGKSRKIPVILVHEPFWRGLVDWFKDRLVSEEMIHPEDLNLIQVIDEPEKVVDA
                     IFNHYQTRGFLPLPDEHELMLNL"
     gene            4715..5053
                     /locus_tag="PLH85_06720"
     CDS             4715..5053
                     /locus_tag="PLH85_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNB64290.1"
                     /translation="MPRIPHFAALMLAVTAGAWAQQPPRLEPLPEPPPPPPGMELDPS
                     LEPQVTIVKKGEDTVEEYRVNGQLYMIKVTPVNGPAYYLYDDVGNGDFLRRDSQDSGV
                     RVPKWVIKRW"
     gene            complement(5143..6972)
                     /gene="sppA"
                     /locus_tag="PLH85_06725"
     CDS             complement(5143..6972)
                     /gene="sppA"
                     /locus_tag="PLH85_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNB64291.1"
                     /translation="MERKSRPGLIRRMLTGLWRGVDRLRQISLNLLFIAVAVFLIAGW
                     WASRPTPLEAGSALLVAPVGQIVEQRTSRSPMSVLQGGDGIHQVQLRDIVDGIRAAAA
                     DSRIKALVIEPDGINTVGLSKLQEIAAAIAEFRKAGKKVYVHAKHYSQSQYYLAAQAD
                     EAFMNPDGYVFLSGFARYPTYYKGLLDSLGVRMQIFRVGTYKSFVEPYTRSDMSPEDR
                     EASRGYLEAAWQIFRQDIAAARPKAADKLDAYINDANAQMAAAGGDAARMARDAGLLD
                     GLKTADEWRDFLKARVGTDGDGKQYRHVDLAAYLAHVREAAPHPDERVGVVVAQGAIV
                     DGDQPAGVVGGDTAALLIRQARENDAIKALVLRVDSPGGSAFASEVIRRELELTRKAG
                     KPVIVSMSSVAASGGYWISTAADEVWASPATLTGSIGIFAMVPDLSGPMNKLGLTVDG
                     FGTTPLAGGLDPRRPLDPKVADLLQQSIDHGYKRFLQVVAQGRRMTPEAVDPIAQGRV
                     WLGTQAKEKGLVDKLGGLDEAVKAAAARAGLTRYDVTYVEKSLSPRDQLLTRLLDSEE
                     ESRASAAASPVDVLLANLRGELANLAHWNDPGFAYLHCLCVAP"
     gene            7074..7487
                     /locus_tag="PLH85_06730"
     CDS             7074..7487
                     /locus_tag="PLH85_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB64292.1"
                     /translation="MFTNPSPDQLRTLLREVKSIAVVGLSPRPDRPSHRVARALQGFG
                     YRIVPVRPAVAEVLGEKAYARLADIPHPVDLVDVFRASDEVAPLVDECIALGVKRLWL
                     QDGVIDEAAAEKAQAAGMTVVMDRCVLRDYVDLIP"
     gene            7484..8323
                     /gene="dapF"
                     /locus_tag="PLH85_06735"
     CDS             7484..8323
                     /gene="dapF"
                     /locus_tag="PLH85_06735"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNB64293.1"
                     /translation="MKQTLAFTKMQGLGNDFVMLDAVRQPVDLSPERVRALADRHFGI
                     GCDQLLVVEPSDTPGVDFRYRIFNADGGEVEQCGNGARCFARFVFEQGLTDKREIRVE
                     TAKGLITPRLEDDGTVTVDMGVPVLTPAEVPFVSDSDAYVQPLDVAGRTVAITAVSMG
                     NPHAVQVVADVDTAPVAETGPLIEHHPRFPARVNAGFLQVVDAHRVRLRVFERGAGET
                     LACGTGACAAVVAGILRELVVSPVTVETRGGELTIAWDGVGTPVMMTGPAVTVFSGEI
                     ALP"
     gene            8357..9007
                     /locus_tag="PLH85_06740"
     CDS             8357..9007
                     /locus_tag="PLH85_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNB64294.1"
                     /translation="MNASDVVQFLKDHPGFFDDNPSLLTELTFTHPHAGQAISLTERQ
                     LLALRERIAQLEDKFAELLEFGEENDGISEKVHRLTLSLLEAEDFESIRQAIYTHLLE
                     DFSVPHVAFRVWNSVLSRSSPEFSPVSEAVRFFTGDLGDPYCGPPNQDEVIAWFGPAA
                     RHIRSVALVPLARDDQPFGLLAMGSEDSERFYPEMGTLYVTRIGEMVACALRRQLG"
     gene            9255..10445
                     /locus_tag="PLH85_06745"
     CDS             9255..10445
                     /locus_tag="PLH85_06745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB64295.1"
                     /translation="MADLILLPGKERSVLRRHPWIFAGSVARLEGRARPGDTVIVKDA
                     QGTPLARAAWSPSSQIRARVWSFDPDLSVDHAWFKRAVAASVARRAAHPWLAGQDGVR
                     LIHGESDGLPGVIADRYGEVVVVQLTSAGSDKWRDAIVAGLAQATGCRAVYERSDSDV
                     RKLEGLEPVTGLVHGALPDAPLTIVEHGVRMEVDVAAGHKTGFYLDQRDNRRLTGQLA
                     AGRDVLNCFCYTGGFSLQALAGGARSVLSIDSSGPALATAARNVALNPQLDAARAEWR
                     EDDVFQALRALRAEGRRFDLIILDPPKFAPSAAHAERAARAYKDINLLGLRLLKEGGI
                     LMTYSCSGGIGLELFQKIVAGAAADVGVDARILHRLCAAPDHPVGLAVPEGEYLKGLA
                     CQVS"
     gene            10567..11094
                     /locus_tag="PLH85_06750"
     CDS             10567..11094
                     /locus_tag="PLH85_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64296.1"
                     /translation="MNASTPVVPEPHVSDATPGEADNPTLRRYRRMLSLLLARYTLRH
                     LVRLYAAFEGDLLLPIVLGEVGLYNVSGMDVDEAARGQFRCGCDEDFRKLVLRPCNAF
                     SISSSTGIPRETVRRKVARLAELGWIVRDSRGGLTITAKAAAECLQPFDRENLADFLN
                     TAERLRTLLTAEIKA"
     gene            complement(11144..11626)
                     /gene="bamE"
                     /locus_tag="PLH85_06755"
     CDS             complement(11144..11626)
                     /gene="bamE"
                     /locus_tag="PLH85_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNB64297.1"
                     /translation="MRSLVTLLCSAAAALGLGACDAVTLKDLKPGVSTGYDVRDRLGT
                     PGIEWRNADGSVTWEYSRQPEGTECFMATIGPDNVLRSLENVLTPQNFARVQAGWDKD
                     QVRRLLGKPRTVQTFALKQEEVWDWRLPPEVSADVFFNVHFDRDGRVTGTSRSVQPRG
                     "
     gene            11756..12871
                     /locus_tag="PLH85_06760"
     CDS             11756..12871
                     /locus_tag="PLH85_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB64298.1"
                     /translation="MKRLLTCCVLAAALAGPARADTVVKIGFAGPLTGPIAHVGKDEQ
                     FGAQLALDDVNAKGLTLNGQKVKFELVPEDDQADPRVATTVAQRLADAGVKGVVGHVT
                     SGAAIPASRIYEQAGIPAITPSSTTPKLTQQGFRSIYRVIANDLQQGAAMARFATGVL
                     KAQRIAIIDDRTAYGQGLADALVESLKGLGVTPVGREFTHDKATDFTAILTRLKATRP
                     DAVFYGGMDAQGAPLLKQMQQLGLKVRFLGGDGVCTAEMIRIAGPALSGDVYCTQAGL
                     AMDRMPGGKDFGARFRKRFNADVQLYAPYSYDATMALVEAMKLANSAEPAKYLPLMQK
                     VSFKGVTGPIAFDARGDSREGGVSLYQFNAGRWESRN"
     gene            12928..14040
                     /locus_tag="PLH85_06765"
     CDS             12928..14040
                     /locus_tag="PLH85_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB64299.1"
                     /translation="MKHTLVALAVLGLGASAVHAQEVVKLGTAAPLTGTQAHLGKDIE
                     NGVRLAVEEYNAKGVTLGGKKVKFEVQAEDDQADPRAATTVAQRLVDSGVKGVVGHLN
                     SGASIPASRIYNDAGLPQISPASTNPKLTQQNFKAVFRTIANDVQQGLAIGKYAATKL
                     GKKIALIDDRTAYGQGLADEVEKAVKASGGQIVGREFTSDKANDFQAILTRIKGQNPD
                     VIVYSGMDTQGGPMLKQIKQLGINAKFITGDGGCTGEIIKLAGDAIGANAYCTQPGLP
                     LEQMPGGKDFNARFKKRFNADVQIYAPYAYDATSAIIEAMKAANSADPARYAPAIAKV
                     NFPGVIGKVAFDAKGDILDGAVTVYQFKGGKWEPTR"
     gene            14132..15064
                     /locus_tag="PLH85_06770"
     CDS             14132..15064
                     /locus_tag="PLH85_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB64300.1"
                     /translation="METLLQQVVNGLVVGSVYALIALGYTMVYGILGLINFAHGDVLM
                     VGALVALEAIELIQARWPDLGAIPTLLAGLGVATATCMALGYTIERTAYRRLRNAPRL
                     APLITAIGMSFLLQTVAMIVWGRNYHTFPQLVSTTPLEPVSGVFITPVQIVILATSAL
                     LMVALVLLVNKTRLGRAMRATAENHRVASLMGVDTNKIIASTFIIGSGLAAVAGVMFS
                     SNYGIAHYAMGFMPGLKAFTAAVLGGIGNLGGAMLGGIVLGLVESIGAGYIEDMSFGF
                     LNSSYQDIFAFAILGIVLIFRPTGLLGERVADRA"
     gene            15069..16169
                     /locus_tag="PLH85_06775"
     CDS             15069..16169
                     /locus_tag="PLH85_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB64301.1"
                     /translation="MNELALAIPYLGRRHPAAARAVVLLLAFAAPLIAILFGKTWVRI
                     LDFAMLYVMLAIGLNLVVGFAGLLDLGYIAFYAVGAYTFAFLASPHFDLHLPFWLVLP
                     LGAAFAALAGIILGLPTLRLRGDYLAIVTLGFGEIVRIFLNNLNHPVNITNGPQGINM
                     IDPLVLFGWDISRPLKIGDEITVNSLFLYYYVFLACVALSVMLVQRLQVSRVGRAWAA
                     IRDDELAAKAIGIDTRNMKLLAFSLGATFGGVSGGLFAAFQGFVSPESFSLMESIAVL
                     TMIVFGGMGNLAGVIVGALALTALPEVLRHVAVPLQQSLFDRVILDPEVLRMLLLSLA
                     MIFMMLLRPAGLIPAHSRYSRPELVLGKESAR"
     gene            16166..16957
                     /locus_tag="PLH85_06780"
     CDS             16166..16957
                     /locus_tag="PLH85_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB64302.1"
                     /translation="MITLLEARGITKRFGGLTALSDVSLTIRKGEVYGLIGPNGAGKT
                     TFFNVLTGAYTAEEGELVFNGTELPAGKPHRVVEAGIARTFQNIRLFRDMTALENVMA
                     GHHIRSRANPFAILLQTRQAREEERLITERAYELLRYVGIERFADTVSKNLSYGDQRR
                     LEIARALATEPQLLALDEPAAGMNATETGRLRDLVQTIRHDGITVLLIEHDVKLVMGL
                     CDRLAVLDFGRKIAEDVPAEVQRNEAVIAAYLGGHSTAAAVKEAS"
     gene            16954..17676
                     /locus_tag="PLH85_06785"
     CDS             16954..17676
                     /locus_tag="PLH85_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB64303.1"
                     /translation="MSDASLLELTGLRVAYGAIEAVKGIDLHLAKGELVALIGANGAG
                     KTTTLNAIAGTLPAAGGEIRYDGARINALPAHRRLRKGLALVPEGRGIFTRLTVEENL
                     RMGAYCRRDAAGVEEDMDRIFSMLPRVKERLQQVAGTLSGGEQQMVAIGRALLSRPRL
                     LLLDEPSMGLAPLVVEKIFEVIRSVAAGGVTILLVEQNAHLALEFCQRAYVMESGAVT
                     LSGTGARLLADPKVRAAYLGEL"
     gene            17759..18241
                     /locus_tag="PLH85_06790"
     CDS             17759..18241
                     /locus_tag="PLH85_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HNB64304.1"
                     /translation="MSVLSVSASSSSLSATRPGGAGGASALSDAERRTVARMQQRDRD
                     VRAHELAHITAGGGLITRGAAYTYETGPDGQRYAVGGDVGIDVSPGRTPQETLARAEQ
                     VRRAALAPADPSAQDRQVAARADQMAFQAQQAIAAQATSGTDPTSDTPPPGSLIDVYA
                     "
     gene            complement(18232..18900)
                     /locus_tag="PLH85_06795"
     CDS             complement(18232..18900)
                     /locus_tag="PLH85_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64305.1"
                     /translation="MNWAESIDLYCERTDASFGSEPLNALTNLAFVAVALWLARRYGR
                     TAPRDARGLIALIAMVGLGSLVFHTVATRWAALLDVGFIAVFVLAYFQRFQCHVLGGT
                     DAAAWRGLGVFLVLAAGFAVLLRWGPPWSLNGSEIYLPPFALLGYCAWQARRRDLAAG
                     RWLMAASGLFLFSLACRVIDQALCPLWPWGTHFGWHLGNAAVLACCMEGLLRHLPGGA
                     ADQA"
     gene            19176..21140
                     /locus_tag="PLH85_06800"
     CDS             19176..21140
                     /locus_tag="PLH85_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64306.1"
                     /translation="MHTPHATRRLPRLGLALAVAVPLVLGGLAFADGSLLKSPQGKGE
                     TERNASGTGTPAATTPTRNRASSGEDGGRVTPSSSSSGSGVTTVSIVGSPTVATGSQT
                     VVTSTAAATLPRYTLLAWNDLGMHCVDGKDYSIMSILPPYNNLHAQLIDRQSGKQVTS
                     GVTLSYESLRDPSGSINTFSAGKTNFWQYVKALYGGSPAPDRGLNLSNPAVSNPTPSS
                     RPAAMSFNTVWRWYEAEGLPILPYDDTLTTAGYTKNYYPMVKVVAKNSTGTVLASTQT
                     VLPVSDEMTCVGCHASNSNRDAARPPVRGWANFTANPEKDWKKNVLRLHDDKHANDPV
                     YQAALLKLQPAASGGLALRADQGTPTLCSSCHGSNALNSPGFTVTVNGRAVKISAFTS
                     AMHTLHGKVKDPVKQVALDTIGNRDSCYQCHPGAVTKCLRGAMSDTPNLDCQSCHGNM
                     GAVGKSTRVGWLQQPNCQSCHHDGKRETVAVTDVTTGALRAVTDTRFATEPNKPATGY
                     SLFRFSVGHGKNQCEACHGSTHAEYSSKNQAWASSHDNDLLQSKAIQGYAGTLTECSV
                     CHTSVPLTTNGGPHGLHTIGQAWVNAHGDRVESSGASACKSCHGADYRGTALSQVKVA
                     KTFSHDGRQYALAAGTQVGCYTCHNGPKGD"
     gene            21356..22243
                     /locus_tag="PLH85_06805"
     CDS             21356..22243
                     /locus_tag="PLH85_06805"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HNB64307.1"
                     /translation="MTPPSEPHGPAGGEGPSSLDPALADALRAVLAPLARLALARGVS
                     CGAVEELLKEMFVDEAMRAQGASASGRMVSRISVATGLNRREVTRLTRAERAPRAVRQ
                     PPSSEVFARWITDPALRDADGRPVELPRLPDERGSPSFETLARSVTRDVHPRSLLDEL
                     CRIGVARHDEATDRVCLLRADFVPDADRGGMLGFLGDNVGDHLNAAVANVTAAVPIHF
                     EQAVFADELSETALVAVKALVRDHWLALVHGAVPALEALIEADRQAGRAQDRRVRIGM
                     YSYAAPQTAPVGPAEESTP"
     gene            22240..23430
                     /locus_tag="PLH85_06810"
     CDS             22240..23430
                     /locus_tag="PLH85_06810"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNB64308.1"
                     /translation="MTTFLTRAGRHFCALLVILFALASCGGGGSGVGDLAGVGTGGTG
                     PAAVGIGPISGFGSVIVGGVRYDDSRAVVTDDEGAPRSRLDLRLGMVAEISGSADFTA
                     RTGVATAIRYGSEVLGPVTSVNTAANRFSVLGVGVDVVADTVFDERLTGVAALGMGDP
                     VEVYGYYNVATGRFTATRVDSATGASRYTLRGTVSGLDTTRKRFALAGLTIDYAAVPA
                     AQTAALANGQALRVSATRAPAGGVWVIDALTGAARASLAEGEAEIVGRIDSLTSARHF
                     SVEGVSVDATAASVSGTPALGLRVQVEGTVKNGVLQAREVEVSDDGQSETEEFDLTDV
                     IQSFDPATQRFRLRGQLVDASGTVSFEDGSRSDLANGVRIECKGSFDAALGAVRARLI
                     HFER"
     gene            complement(23446..24660)
                     /locus_tag="PLH85_06815"
                     /pseudo
     CDS             complement(23446..24660)
                     /locus_tag="PLH85_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(24687..25085)
                     /locus_tag="PLH85_06820"
     CDS             complement(24687..25085)
                     /locus_tag="PLH85_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64309.1"
                     /translation="MSVSPAPAAPRSSKKWLVSLIGFFVIVAALVAAYTWLVLSWSYS
                     NGERAGYVQKFSKKGWLCKTWEGELAIVAIPGSLPEIFRFTVRDEKVATDINAVMGKR
                     VSLTYDQHVGIPTTCFGETGYFVSQVHAVE"
     gene            complement(25104..26603)
                     /locus_tag="PLH85_06825"
     CDS             complement(25104..26603)
                     /locus_tag="PLH85_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNB64310.1"
                     /translation="MSLALLKSRALAGMQAPEVIVEVHLANGLPAFTLVGLPDMEVRE
                     ARDRVRAALQTSQFEFPQRRITVNLAPADLPKEGGCFDLPIALGILAASGQIPAEALA
                     GYEFAGELSLTGELRPVRGALALARQAAGSGRRLILPTANAAEAALVDGLVLLPAASL
                     LDVAAHLCGHTPLAPYAGAPLLAEVSYPDLADVKGQAPARRALEVAAAGRHSLLLFGP
                     PGTGKSMLAQRLPGLLPPMDDVEALESAAIQSVDGSFRPERWRQRPWRSPHHGASVAA
                     IVGGGSTPRPGEISLAHQGVLFLDELPEFDRRVLEALREPLENGHITVSRAARRADFP
                     ARFQLVAAMNPCPCGYLGHPDGRCRCTPDQVARYRGKLSGPLLDRIDLLLEVPALPAA
                     DLQAAPAGEPTAAVRERVATARAVQQARQGKANADLGGAEVDRWCRPDAAGVNLLQTA
                     IARLGLSARGYHRILRVARTIADLAGRPDVGAAHVAEAVQSRRGLPGTV"
     gene            complement(26674..27891)
                     /locus_tag="PLH85_06830"
     CDS             complement(26674..27891)
                     /locus_tag="PLH85_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64311.1"
                     /translation="MSVPLLAACAGIGPPAIDQTRLQYNEALKRTAEEQLLLNIVRLR
                     YTDTPSSLAVTGIAAQFEVAQSLGLLPFFTAAGGDLNRSFTAMLPQAQATRADRPTFS
                     FTPLDDQEFTRKLFTPLPLEGLVYLAKTTWPISTVFRLYLENLNWVPNAQTASGPTPR
                     QAPPINDFLEGVHALQRLQDRGDVVFGVEEQEELLGGPLPATAVTPRDVIEAARSGYV
                     YRPDEDGRSWSLVRRFTRPTLQIAPSAVNAEATQRFLSAFRLKPGLLQYAVTQEALPP
                     FREQQPRGGLTQIDLETRSLLQALYYVSLGVDVPPAHLERGVVTLTRDADGTPFDWQR
                     VMIGLFRVRASAADARPADAHVAVRYRDHWFYVDDRDTDSKSTFSLLMELARLELAGK
                     TGPGPQLTLPVGR"
     gene            complement(28032..28607)
                     /locus_tag="PLH85_06835"
     CDS             complement(28032..28607)
                     /locus_tag="PLH85_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB64312.1"
                     /translation="MSALRCALAALLTVGGVLPVRAEPVSYSIDPNHTHVIFETDHLG
                     LSTQRGRFSRSSGKIVLDRAARSGTVDVSLDAAALDMGFAKWNENMRGENFFNVAEFP
                     AVSFRSTRLVFDGDTPVAAEGTVTLLGVSRPLTLTLQRVHCAPNPLNKRETCGADITG
                     RLKRSEFGMTRNIPSVSDEVRLIIAVEAFRD"
     gene            complement(28611..28871)
                     /locus_tag="PLH85_06840"
     CDS             complement(28611..28871)
                     /locus_tag="PLH85_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNB64313.1"
                     /translation="MANPKILDEIGSKVSELLANSPARDLEKNARALLASGFSKLDLV
                     TREEFEVQRDVLARTREKLAELEHRVAHLEAELAKRPPAEGV"
     gene            29145..>29375
                     /locus_tag="PLH85_06845"
     CDS             29145..>29375
                     /locus_tag="PLH85_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNB64314.1"
                     /translation="MRKTVLASALLASFVAAPAVSFAEDAAPAAPAFTSNVTLASEYI
                     YRGIGQTNRKPAIQGGFDYAHSSGLYVGTWASN"
     assembly_gap    29376..29475
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29573..29911
                     /gene="glnK"
                     /locus_tag="PLH85_06850"
     CDS             29573..29911
                     /gene="glnK"
                     /locus_tag="PLH85_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNB64315.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVQTEILDQVIEAIEKSASTGKIGDGKIFVFDLEQAIRI
                     RTGESGNEAL"
     gene            29927..31453
                     /gene="amt"
                     /locus_tag="PLH85_06855"
     CDS             29927..31453
                     /gene="amt"
                     /locus_tag="PLH85_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNB64316.1"
                     /translation="MKKFVAVLLTAFAVNFAGGALADDKPAAPAAAVTAPAEAKPAEA
                     PAAEAKPAEAAPAAAAEAAPAEAAAAPVPPNKGDNAWLIVATAFVILMSIPGLALFYG
                     GLVRAKNMLSVLLQVFTVFSLISVLWVLYGYSVAFTEGNAFFGGLDKVFLKGITPDSV
                     AATFSKATNISEFIYVAFQGAFAAITVALILGAFAERIKFSAVLLFSVLWFTFSYLPI
                     AHMVWFWAGPDAYTSAEAADAANATAGWLFQKGALDFAGGTVVHINAAVAGLVGAFLV
                     GKRIGFGRESFAPHSLTLTIVGASLLWVGWFGFNAGSALEANGVAALAFVNTWVATAA
                     AAMSWLLAEWIIKGKPSGLGAASGAVAGLVAITPACGFVGVVGAIVIGLLAGVVCLWG
                     VNGLKRMLGADDSLDVFGVHGVGGILGATLTGVFAAPSLGGSGIWDYVANKAADAYSI
                     GDQVVIQLTAVGTTIVWSAVVAFISYKLVDMFIGLRVPEDEEREGLDVTSHGETAYHQ
                     "
     gene            31604..33271
                     /locus_tag="PLH85_06860"
     CDS             31604..33271
                     /locus_tag="PLH85_06860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB64317.1"
                     /translation="MFRSPARSPRWAAVIVALVLLVPPLHASAREAVTLQLKWRHAFQ
                     FAGYYAAKEKGFYDAAGLDVHLQEATPGMDPVEPVLNGQAQYGVGNSSLLLARRAGQP
                     VVVLAVVFQHSPLVLLTRQQPDTADAQGLQHLAGKRVMIEPHSDELIAYLKREGVPLD
                     QLVQVPHSYRPEDLIEGRVDAITAYASNEPFYLDRAGLAYQSYSPRAAGIDFYGDNLF
                     TTEDELRNHPDRVRAFRDASLRGWHYALEHPTEIADLLHRHYAPQHPRAFFLFEAQRM
                     MPLLRADLIEVGYMNPERWRHIADTYADLGLLPRDYALDGFLYAPDVQLDRSRFYLTL
                     ALLALVTGAAMYVVRINRRLSRVLAESQAAEARIRHLAQHDPLTELPNRALLSDRLGQ
                     ALVAAKRDTRRLAVMFIDVDNFKPINDTLGHAVGDRLLQQIAKRLQASLRESDTVARI
                     GGDEFVVLLRNVTDGPDALAVADKLREVLSVPIVVEAHTLVVGASVGVALYPDHGSDA
                     LELFRHADEAMYRAKRDGRNRAQVFEARSDRGQPLAPSQPAASSVGP"
     gene            complement(33366..33977)
                     /locus_tag="PLH85_06865"
     CDS             complement(33366..33977)
                     /locus_tag="PLH85_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64318.1"
                     /translation="MRDFLDKVRTLRWDDHRYYHQSRINQTLHLISAISFLVAYGLLF
                     SDPALAGLVGWLVGMVTRQSGHIFYEPRGFDAIHNTTYAHKESIKVGYNMQRKGVLIG
                     VWLSIPVLLLVSPDAFGLLDAGGGAAGYLHNLGLLWLCLGAGGLGFRTLHLFFLRGPV
                     WGLAWAFKILTDPFHNIDIYWRSPIALMRGQLLDPLDGEEAHA"
     gene            complement(34198..>34605)
                     /locus_tag="PLH85_06870"
     CDS             complement(34198..>34605)
                     /locus_tag="PLH85_06870"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNB64319.1"
                     /translation="RAFAEVQRIFEEVHGDTMVLARYMQILSPDLCAAHPGRIINIHH
                     SFLPSFVGAKPYHQAYDRGVKLIGATCHYVTADLDQGPIIEQDVIRIDHSDSVEDMVR
                     YGKDIEKAVLARGLRYHLEDRVLVHGNKTVVFR"
BASE COUNT         5510 a        12069 c        11589 g         5337 t
ORIGIN      
        1 cccaggagcc ggtgagctgc tcggccttca cgtcgccgcc gtccacgatc ttcagcgcgt
       61 cgtagaactc gccgcggaag taggcctggc ggcgcccgta acaggacagg gtagacaggg
      121 aggtgagcag cgggcgggtg ccgaagttgc ggccgaagtt gctctccacg ccccacacgg
      181 cgacgacggt ggcggggtcc acgtggtagc gggcagccac ggcggcgagg gtgtcgcccc
      241 actgcttgag catcgcctgc ccgtcggcga cgcgttcgtc gtccaccagg ccggagaggt
      301 aatcccagat cggggtgcgg aattcgggct ggtagtcgag aaaatccagc accttgagat
      361 ccggctccag gccggcggtg aggcgttcgt agccggcagg cgagacgccc cgcgcgacgg
      421 cgccggcctt gaggcccgcg acgcaggggg cgaactgggc gacatcggcg gcgcgggcga
      481 caggcacgcc caggacgagg gccgccacgc cggcggccag ggtggagcga agagtcatca
      541 gcggatgtag gtctgcatca gggcgaagaa gcgcccgtag aaatcggaat cggtgatggt
      601 ctcttcaccg accttgacga gggattcgcc gttgccgccc aggggcaggt tgagggagcc
      661 gatgccggcg acgctgactc cggcgctgct ggaactggcc ttgagctcgt cgcggacttc
      721 gcgggcattg gcgaagactt cggtgccgtc gccgtccgcc aggcaggaga ggacgaagtt
      781 gatctgcacg tgcacgttgc gctcgggctg gaagaacttg cgcgccttga ccgcgtcggg
      841 cttggcgtcc tcgatcacgt agccctggct gagcagggcg cgccggccgg cgtcgcaggc
      901 cgcggtcggc gccacgtcca ccgccacctc gtagggcgag ttggtgttga agcgctcgct
      961 gcggtaggtc aggtctttct tgggggcgct gctgcaggcg ccaagggcca gcgcggcgca
     1021 gctgaggagg aacagaacgg gcaggcgggc ggagcggtgg tgctgcatgg gcggatctcg
     1081 tggggtgggc ggagggtcgg cgcgaaccgg gccgggcgcg gaggagccgt ccgcgcccgg
     1141 cgcacgcatt gcgatcagtg ggcctcgtcc cagttcgcgc cgacccccac ttcggcaacc
     1201 agcggcacgg aaagttgcgc gacgcccgcc atgagctggg gcagccgctc gcggatcaga
     1261 gcgacctcgt cgtcgggcac ttccagcacc agttcgtcgt ggacctgcag gatcaggcgc
     1321 gcccgcacgc cggccgtttt gagccaggca gacacggcga tcatggcctt cttgatcaga
     1381 tccgcggcgg tgccctgcat gggcgcgttg atggcggccc gttcggcagc ctggcgccgc
     1441 cccacctgct gggcgcggat ctccggcagg tacaggcggc ggccgaagac ggtctccacg
     1501 tagccctgtg tccgggcttc ggtgcgggtg cgctccatgt aggcggcgac gccggggtat
     1561 cgggcaaagt agcggtcgat gtaggttgcc gcggcggccc gctcgatgcc caggtttctg
     1621 gccaggccgt gggcgctcat gccgtagatc agcccgaagt tgatcacctt ggcggtgcgg
     1681 cgctgctcgc tgccgacttc catgggcgtg atgccgaaga tctcggcggc ggtggcccgg
     1741 tgcacgtcct cgccgtgggc gaaggcgttc agcaggccgg cgtcgccgga caggtgggcc
     1801 atgatgcgca gctcgatctg ggagtagtcc gccgagacga tgcggtggcc ggccggggcg
     1861 atgaaggcgg cgcggatgcg gcggccttcc ggcgtgcgga tggggatgtt ctgcaggttg
     1921 ggctcggtgc tggccaggcg cccggtgacg gccaccgcct ggctgaagct ggtgtgcacg
     1981 cgcccggtgc gcgggttgac catcagcggc agcttgtcgg tgtaggtgtt cttgagcttg
     2041 gccaggctgc ggtgctccag cagcagcttg ggcagcgggt agtcctcggc gagctgggtg
     2101 agcacgtcct cgtcggtgga cggctggccg gtggcggtct tcttgaccac cggcaggccc
     2161 agcttggtga acaggatctc gccgagctgc ttgggcgagc ccaggttgaa gggctggccg
     2221 gcgagggcgt gggcgtcgag ctccagcgcg tgcaggcgac gcccgagctc ctcggaatgc
     2281 tgggcgagga ggaagtcgtc gatgagcacg ccggtgcgct ccatctcaaa gagcacccgc
     2341 gcggtgggca gctccacgtc ccggtagagg gcggcgaggg cgggctcggc ggcgagctgg
     2401 gcggacaggg cgtggtgcag gcgcagggtg atgtccgcgt cctcggcggc atattcggtg
     2461 gcgcgggcca ggtccacctc gccgaagccg atctgcttgg cgcccttgcc gcagacttcg
     2521 gcgtaggtca gggtcttgag gcccaggtga cgactggcga gggaatccat gtcgtgggac
     2581 ttgtcgcttt ccagcacgta ggattccagc agggtgtcct cggcgatgcc ggcgagccgg
     2641 atgccgtggt tggcgagaat gtgggcgtcg tacttgaggt tctggccgag cttgcggtga
     2701 gcgtcggact ccagccaggg cttgaggcgg gccagcacct cggccagggg cagctgggcc
     2761 ggcgcgtcgg cgtaggtgtg cgccagcggc aggtaggcgg cctggcccgt gacggtggcg
     2821 aaggacatgc ccacgagagt cgcgaccagc gggtcgaggc cggtggtctc ggtgtcgaag
     2881 gcggtcagtt cggctgcctg gatctgtgcc agccaggcgt cgaaggcgtc ccagtcgagc
     2941 agggtggcat agccctgacg gtgggcaccg ggcgcggccg gctcggcgga cggcggtgga
     3001 gcgtcgccgg cggggggcgt ctcggaggca ccggcgggcg gggcggattc gagctccttc
     3061 agccagctct tgaactccat gcgccggtag agctcagcca gcgccgcccg gtcggccggt
     3121 cgcaccgcca gatcggccgg gccgacgggc aggggcagat cggtgacgac ggtgacgagc
     3181 ttgcgcccga ggggtaggaa gtccagatgc ttgcgcaggt tctcgccgac cttgccgccc
     3241 acctcggcgg cgtgggccac gagggtgtcc aggtcgccgt actggctgag ccacttgacg
     3301 gcggtcttgg ggccgcattt ctcgacgccc ggcacgttgt ccacggtgtc gccgatgagg
     3361 gccaggtaat ccacgatgcg gcccggcggc acgccgaact tggcctccac gccggctgcg
     3421 tcgagaactt cctcgctcat ggtgttcacc cagcgcacgc ccggtcccac caactgggtg
     3481 agatccttgt cgccggtgga gatgaccacc tcccagccgg cctccgcggc ctggcgggtg
     3541 agggtgccga tcacgtcgtc ggcctcgacg ccgtcttcca tcagcagcgg ccagccttct
     3601 gcgcgtacgg cggcgtggag gggctcgatc tgggcgcgca gatcctcggg catgggcggc
     3661 cgctgggcct tgtaggcggg gaaccagtcg tcgcggaagg tcttgccctt ggcatcgaag
     3721 acgcaggcgc ggtagtccgc cttgtagtcg gattcgaggc gacgtagcat ggcgagcacc
     3781 ccgcgcacgg cgcccgtggg ttcgccggcc gagttgcgca ggtcgggcag ggcgtggaag
     3841 gcgcggtaca ggtagctgga accgtcgacg agcagcaggg tcggcatggg caagcccggg
     3901 cccgacggca cgatgccggc ggcccctatt cctcagaaga aaaatgacag caaaagacaa
     3961 gctcccgaag atggcgccgg ggtcggaatc ccaacacttc acggcccggg agtcgtggcg
     4021 gattttcggg attatggcag agttcgtcga ggcgaccgaa cggctcaagc cgatccgccc
     4081 ggcggtgtcg atcttcggca gcgcccgcgt caaacccgac catccctact acgcccagac
     4141 cgaacgcatc gcgcgactgc tgtcggacgc gggcttctcg gtgatctccg gcggcgggcc
     4201 gggcatcatg gaggcggcca acaagggcgc ctatttcggc aagtcaccca gcgtggggct
     4261 caacatccag ctccccatgg agcagcaggc gaatccctat caggacattt cccagacctt
     4321 ccagcacttc ttcgcgcgca agttcatgtt cgtgaagttc gcgtcggcct acgtggtgat
     4381 gccgggcggc ttcggcaccc ttgacgagct gctggaggcg atgaccctga tccagaccgg
     4441 caagagccgc aagatcccgg tgatcctggt gcatgagccc ttctggcgcg ggctggtgga
     4501 ctggttcaag gaccgcctgg tgagcgagga gatgatccac ccggaggatc tgaatctgat
     4561 ccaggtgatc gacgagccgg agaaggtggt ggacgccatc ttcaaccatt accagacccg
     4621 cggcttcctg cccctgccgg acgaacacga gctgatgctg aatctgtaag cgccggagcg
     4681 cggggtacaa tgccggcatc ttgaggagaa caccatgccc cgcatcccgc atttcgccgc
     4741 tctgatgctg gcggtcaccg ccggcgcctg ggcccagcag ccgcccaggc tcgaacccct
     4801 gcccgaaccg cccccgccgc cgcccggcat ggaactggac ccgtccctgg agccccaggt
     4861 caccatcgtc aagaagggcg aggacacggt ggaggagtac cgggtcaatg gccagctcta
     4921 catgatcaag gtcaccccgg tgaacggccc ggcctactac ctctacgacg acgtgggcaa
     4981 cggcgacttc ctgcgccgcg acagccagga ttccggcgtg cgcgtgccca agtgggtgat
     5041 caagcgctgg tgaggccctg aaacgccgga cccggccgca gccgggtccg atcaatggac
     5101 tgcgctgcgc cgggcggcct caccgcccgg cggcgccccg gatcagggcg ccacgcacag
     5161 gcagtgcagg taggcgaagc ccggatcgtt ccagtgggca aggttcgcca gttcgccccg
     5221 caggttggcc agcaggacat ccaccggtga cgcagcagcg cttgcgcggg attcctcctc
     5281 gctgtcgagc agccgggtga ggagttgatc gcggggcgac agggatttct ccacgtaggt
     5341 cacgtcgtat cgggtgaggc cggcccgggc ggccgcggcc ttgaccgctt cgtcgagccc
     5401 gccgagcttg tccaccagac ccttctcctt ggcctgcgtc cccagccaca cgcgcccctg
     5461 ggcgatcgga tccaccgcct cgggcgtcat ccggcggccc tgggccacca cctgcaggaa
     5521 gcgcttgtaa ccgtggtcga tggactgctg gagcaggtcc gccaccttcg ggtccagcgg
     5581 ccggcgcgga tccagtccgc ccgccagcgg tgtggtgccg aaaccgtcca ccgtgaggcc
     5641 cagcttgttc atcggcccgg agaggtccgg caccatcgcg aagatgccga tggagccggt
     5701 cagggtcgcc gggctggccc acacctcgtc ggcggcggtg ctgatccagt aaccgccgga
     5761 agccgccacc gagctcatgg agacaatcac cggcttgccg gccttgcggg tcagctccag
     5821 ttcgcggcgg atcacctcgg aggcgaaggc gctgcccccc gggctgtcga ctcgcagcac
     5881 cagggccttg atggcgtcgt tttcccgcgc ctgacggatc agcagggccg cggtgtcgcc
     5941 gcccaccaca ccggcaggct gatcgccgtc cacgatggcg ccctgggcca ccaccacgcc
     6001 gacacgctcg tcggggtgag gcgcagcctc gcgcacgtgg gcgaggtagg ccgccagatc
     6061 cacgtgcctg tactgcttgc cgtcgccgtc cgtgccaacg cgcgccttga ggaagtcgcg
     6121 ccactcgtcg gccgtcttga ggccgtccag caggcccgcg tcccgcgcca tgcgggccgc
     6181 gtcgccgccg gcggcggcca tctgcgcatt ggcgtcgttg atataggcgt cgagcttgtc
     6241 ggcggccttg ggacgggccg ccgcgatgtc ctgacggaag atctgccagg ccgcctccag
     6301 gtagccgcgg ctcgcttccc ggtcttccgg tgacatgtcg ctgcgggtgt agggctcaac
     6361 gaaggacttg taggtgccca cccggaagat ctgcatgcgc acccccagac tgtcgagcag
     6421 gcccttgtag taggtgggat agcgcgcgaa gccggacagg aagacgtagc cgtccgggtt
     6481 catgaaggcc tcgtccgcct gggccgccag gtaatactgg ctctggctgt aatgcttggc
     6541 atgaacatag accttcttgc cggccttgcg gaattcggca atggccgcgg cgatctcctg
     6601 cagcttggac aggccgacgg tattgatgcc gtccggctcg atgaccagcg ccttgatgcg
     6661 cgagtcggcc gccgcggcgc ggatgccgtc cacgatgtcg cgcagctgca cctggtggat
     6721 gccgtcgccg ccctggagca cgctcatggg actgcgcgag gtgcgctgct cgacgatctg
     6781 gcccaccggc gccaccagca gggcgctgcc tgcttccagc ggcgtggggc ggctcgccca
     6841 ccagccggcg atcagaaaga ccgcgactgc gatgaacaac aggttcaggc tgatctgccg
     6901 gaggcgatcc accccccgcc acaggccggt cagcatcctg cggatcagac cgggcctcga
     6961 cttgcgttcc attgtgctct ccgttgctgc ggcccggtgc gcgggcctgt tatgcgggga
     7021 ccggtggcgg cgtccaaggt ttcgacgccg cggcaatccg gtagaatccc gccatgttta
     7081 ccaatccatc ccccgaccag ctccgcacac tgctgcggga ggtcaagtcc atcgccgtgg
     7141 tcggcctgtc gccgcgcccg gatcgcccca gtcaccgggt cgcccgcgcc ctgcagggtt
     7201 tcggataccg catcgtaccg gttcgacctg ccgttgccga agtgctcggt gaaaaagcct
     7261 acgcccgcct ggcggacatt ccccacccgg tggatctcgt ggacgtcttc cgggcgtcgg
     7321 acgaggtcgc accgctggtg gacgaatgca tcgccctggg tgtcaagcga ctgtggctgc
     7381 aggacggggt gatcgacgaa gcggcggccg aaaaggcaca ggcggcgggc atgaccgtgg
     7441 tgatggaccg ttgcgtgctg cgggactacg tggatcttat cccatgaagc agactctcgc
     7501 cttcaccaaa atgcaggggc tgggcaacga cttcgtcatg ctcgacgccg tgcgccagcc
     7561 cgtagacctg tcccccgagc gggtgcgtgc gcttgccgac cggcatttcg gcatcggctg
     7621 cgaccagttg ctggtggtcg agccatccga tacgccgggc gtggatttcc gctaccgcat
     7681 cttcaatgcc gacggcggcg aggtggaaca gtgcggcaac ggcgcccgct gcttcgcccg
     7741 cttcgtcttc gaacagggcc tcaccgacaa gcgcgagatc cgcgtcgaga ccgccaaggg
     7801 cctcatcacc ccgcgcctgg aggacgacgg cacggtcacc gtggacatgg gcgtgccggt
     7861 gctcaccccg gccgaggtgc ccttcgtcag cgattccgac gcctacgtcc agcccctcga
     7921 cgtggccggc cggaccgtcg ccatcactgc cgtgagcatg ggcaatcccc acgccgtgca
     7981 ggtggtggcg gacgtggaca cggcgccggt ggccgagacc ggccccctca tcgagcacca
     8041 tccgcgcttc ccggcgcggg tcaacgcggg cttcctgcag gtggtggacg cccaccgcgt
     8101 gcggctgcgc gtcttcgaac gcggtgccgg cgagaccctc gcctgcggca cgggcgcctg
     8161 tgccgccgtg gtggccggca tcctgcgcga actcgtggtc tcgccggtca ctgtcgagac
     8221 ccgcggcggc gagctcacca tcgcctggga cggcgtcggc acgccggtca tgatgaccgg
     8281 cccggccgtc accgtgttct ctggcgaaat cgcccttccg tgaccccgtg acccactcct
     8341 ctcccggcgc ccgcccatga atgcatccga cgtcgtccag ttcctcaagg accatcccgg
     8401 cttcttcgac gacaacccgt cgctgctgac cgagctcacc ttcacccacc cccacgccgg
     8461 ccaggccatc tccctcaccg aacggcagct cctcgccctg cgggagcgca tcgcccagct
     8521 ggaggacaag ttcgccgagc tgctggagtt cggcgaggag aacgacggca tcagcgagaa
     8581 ggtgcaccgc ctgaccctgt ccctgctgga agccgaggac ttcgaatcca tccgccaggc
     8641 catctacacc cacctgctgg aggatttctc ggtgccccac gtggccttcc gggtgtggaa
     8701 cagcgtgctg tcccgcagca gcccggaatt ctcgccggtc agcgaggcgg tgcgcttctt
     8761 caccggcgac ctgggcgacc cgtattgcgg cccgcccaat caggacgagg tcattgcctg
     8821 gttcggcccc gcggcccggc acatccgctc cgtcgccctg gtgcccctcg cccgcgacga
     8881 ccagcccttc ggcctgctgg ccatgggcag cgaggactcc gagcgcttct atcccgaaat
     8941 gggcacgctc tacgtcaccc gcatcggcga gatggtggcc tgtgccctcc gccgccagct
     9001 cggctgaggg gtgcataaag ctactgcgcc ggccgatctc gcgactgcga tgctcgccgt
     9061 acgtcttgta cggctgcgct tctcgtcacg acctcgaccg gctcgcgacg ctttctgcac
     9121 ccctctgacg cggggtcggg cgatccggcc cggacgcgct ttcctcactc cgaactcatt
     9181 gcacgtgctc agcgcacgcg attccgacgc ggcgcccaac cgcgtgccgc tcaaccccac
     9241 tgaattgctt cgtcatggct gacctgattc tcctccccgg caaggaacgt tccgtgctcc
     9301 gtcgtcaccc ctggatcttc gccggctcgg tggcgcggct ggaggggcgg gcgcggccgg
     9361 gcgacacggt gatcgtcaag gatgcccagg gcacgccgct ggcgcgggcg gcctggtcgc
     9421 cgtcgtccca gatccgggcg cgggtgtgga gcttcgatcc ggacctgtcg gtggatcacg
     9481 cctggttcaa gcgggcggtc gcggcctcgg tggcgcggcg ggcggcgcat ccgtggctgg
     9541 cggggcagga cggcgtgcgc ctgatccacg gcgaatccga cggcctgccc ggcgtcatcg
     9601 ccgaccgcta cggcgaggtg gtggtggtcc agctcaccag cgccggttcg gacaaatggc
     9661 gcgatgcgat cgtcgccggg ctggcccagg ccaccggctg ccgcgcggtc tacgagcgct
     9721 ccgactcgga cgtgcgcaag ctcgaaggcc tggagccggt caccggcctg gtgcatggcg
     9781 cgctgccgga tgcgccgctc accatcgtcg agcacggcgt gcgcatggag gtggacgtgg
     9841 ccgcgggcca caagaccggc ttctacctgg atcagcgcga caaccgccgc ctcaccggcc
     9901 agcttgccgc cggccgggac gtgctcaact gcttctgcta cacgggcggc ttttccctgc
     9961 aggccctggc tggcggcgcc cgcagcgtgc tgtccatcga ctcctccggg ccggcgctgg
    10021 ccaccgcggc gcgcaatgtg gcgctcaacc cgcagctcga cgccgcccgc gccgaatggc
    10081 gcgaggacga cgtcttccag gcgctccgcg cactgcgcgc cgagggacgc cgtttcgacc
    10141 tgatcatcct cgacccgccc aagttcgccc cctccgccgc ccacgcggag cgcgccgcgc
    10201 gggcctacaa ggacatcaat ctcctcggtt tgcgtttgct caaagaaggg ggaatcctga
    10261 tgacctactc ttgctccgga ggcatcgggc tggagctgtt ccagaagatc gtggccggag
    10321 ctgcggccga cgtgggggtg gatgcgcgga tcctgcaccg gctctgcgcg gcaccggatc
    10381 accccgtcgg cctcgcagtg ccggaaggtg aatatctgaa agggctggcc tgccaggtgt
    10441 catgagagtg cccaaagcgg gtagtccggg ggtttgagcc gcggcgcggg tgtgtatcct
    10501 gcgcggcttg tcgccgggag cgccgcacag ggcacaccgt accggtggtc cctttcggag
    10561 agctccatga atgcctcgac gcccgtagtg cccgaaccgc atgtttccga tgcgacaccc
    10621 ggcgaggccg acaaccccac cctgcgtcgc taccggcgga tgctgtcgct gctcctggcg
    10681 cgctacaccc tgcgccatct ggtgcgcctc tacgcagcct tcgagggcga cctgctgctg
    10741 cccatcgtgc tgggtgaagt gggtctctac aacgtcagcg gcatggacgt ggacgaagcg
    10801 gcgcgcggcc agttccgctg cggctgcgac gaggatttcc gcaagctcgt gttgcggccc
    10861 tgcaatgcgt tctccatcag ttcctccacc ggcatcccgc gcgagaccgt ccgccgcaag
    10921 gtggcgcgcc tggcggaact gggctggatc gtgcgcgaca gccggggcgg cctgaccatc
    10981 acggccaagg ccgcggcgga atgcctgcaa cccttcgacc gggaaaatct cgccgacttc
    11041 ctgaacaccg ccgagcgcct gcgcacgctg ctcacggccg aaatcaaggc ctgaacgcct
    11101 ttccggatga cggccccgcg tgcggacccg gggcgcggct tgctcaaccg cggggctgca
    11161 cgctgcggct ggttccggtg acgcgtccgt cccggtcgaa gtgcacattg aagaacacgt
    11221 ccgcgctcac ctccggcggc agacgccagt cccacacctc ttcctgcttg agcgcaaagg
    11281 tctgcacggt ccgcggcttg cccagcagac ggcgcacctg atccttgtcc caacccgcct
    11341 gcacgcgggc gaagttctgc ggcgtgagga cgttctcgag gctgcgcagc acattgtccg
    11401 gcccgatggt ggccatgaag cattcggtgc cctcgggctg acggctgtat tcccaggtca
    11461 cgctgccgtc cgcgttgcgc cattcgatgc ccggcgtgcc aaggcggtcg cgcacgtcgt
    11521 agccggtgga cacgccgggc ttgaggtcct tcagggtgac cgcgtcgcag gcccccaggc
    11581 cgagggcggc ggctgcgctg cacagcaggg tgacgaggga acgcatggcg ggcacttcct
    11641 ttccgacgcc ggccgttcaa acccggcagg cttggttaca attcccggct gcggcggtcc
    11701 gggcaaggtc cgggccgatt ctggcacgaa tgccccttgc ggagaaccga ccccgatgaa
    11761 acgcctgctc acctgctgcg tcctggccgc tgccctcgcg ggccccgccc gcgcggacac
    11821 ggtggtgaag atcggcttcg ccggccccct caccgggccc atcgcccacg tgggcaagga
    11881 cgaacagttc ggcgcccagc tcgccctcga tgacgtcaac gccaaaggcc tcacgctcaa
    11941 cgggcagaag gtgaagttcg agctggtgcc cgaggacgac caggccgacc cccgtgtcgc
    12001 caccacggtg gcccagcgcc tcgccgacgc cggcgtgaag ggcgtggtcg ggcacgtcac
    12061 ttctggcgcg gcgattcccg cctcgcgcat ctacgagcag gccgggattc ccgccatcac
    12121 cccgtcgtcc accacgccca agctcaccca gcagggcttc cgcagcatct accgggtcat
    12181 cgccaacgac ctgcagcagg gcgccgccat ggcacggttc gccaccggcg tgctcaaggc
    12241 ccagcgcatc gccatcatcg acgaccgcac ggcctatggc cagggtctcg ccgacgcgct
    12301 ggtcgagagc ctcaaaggcc tcggcgtgac gccggtcggg cgggaattca cccacgacaa
    12361 ggccaccgac ttcaccgcca tcctcaccag gctgaaggcc acccgccccg acgcggtgtt
    12421 ctacggcggc atggacgccc agggcgcgcc gctcctcaag cagatgcaac agctcggcct
    12481 caaggtgcgc ttcctcggcg gcgacggcgt gtgcaccgcc gagatgatcc gcatcgccgg
    12541 cccggccctg agcggcgacg tgtattgcac ccaggccggc ctggccatgg accgcatgcc
    12601 cggcggcaag gacttcggcg cccgcttcag gaagcgcttc aacgccgacg tgcagctcta
    12661 cgcgccttac agctacgatg cgacgatggc cctcgtcgag gcgatgaagc tggccaactc
    12721 ggccgagccg gccaaatatc tgcccctgat gcagaaggtg tccttcaagg gcgtcaccgg
    12781 cccgatcgcc ttcgatgccc gcggcgacag ccgcgaaggg ggcgtgagcc tctaccagtt
    12841 caacgccggc aggtgggaat cccgcaactg accggccccg atattgcttg accgtccttg
    12901 ccgtccccat ccacaggaga atcgcccatg aaacacaccc tcgtcgcact cgccgtgctc
    12961 ggtcttggtg catcggccgt ccacgcccag gaagtcgtca agctcggtac cgccgcgccc
    13021 ctcaccggca cgcaggccca cctgggcaag gacatcgaga acggtgtgcg gctggccgtc
    13081 gaggaataca acgccaaggg cgtgaccctg ggcggcaaga aggtcaagtt cgaagtccag
    13141 gccgaggatg accaggccga cccccgcgcc gccaccaccg tggcccagcg ccttgtggac
    13201 agcggcgtga agggcgtcgt cgggcacctc aactccggcg cctcgattcc cgcctcgcgc
    13261 atctacaacg acgccggact gccccagatc tcccccgcct ccaccaatcc caagctcacc
    13321 cagcagaact tcaaggcggt cttccgcacc atcgccaacg acgtgcagca gggcctcgcc
    13381 atcggcaagt acgccgccac caagctgggc aagaagatcg ccctcatcga cgaccgcacg
    13441 gcctacggcc agggcctggc ggacgaggtg gagaaggccg tcaaggccag cggcggccag
    13501 atcgtcggcc gcgaattcac cagcgacaag gccaacgact tccaggccat cctgacccgc
    13561 atcaagggcc agaacccgga cgtgatcgtt tatagcggca tggacaccca gggcggcccc
    13621 atgctcaagc agatcaagca gctgggcatc aacgccaagt tcatcaccgg cgacggcggc
    13681 tgcaccggcg agatcatcaa gctggcgggc gacgccatcg gcgccaacgc ctactgcacc
    13741 cagcccggcc tgcccctgga gcagatgccc ggcggcaagg acttcaacgc ccgcttcaag
    13801 aagcgcttca atgccgacgt gcagatctac gccccctacg cctacgacgc cacctcggcc
    13861 atcatcgaag ccatgaaggc tgccaactcg gccgatccgg ccaggtatgc gccggccatt
    13921 gccaaggtga acttccccgg cgtgatcggc aaggtcgcct tcgacgccaa gggcgacatc
    13981 ctggacggtg ccgtcacggt ctaccagttc aagggcggca agtgggaacc cacccgctga
    14041 tctgagtctt ccgaccgcgg ggtgcgccgg ccggtgcgcc ccgcccggac cggcccgcgc
    14101 cggccttccc ttcccgttcc gggtcgcttc gatggaaacc cttctgcaac aggtggtcaa
    14161 tggactggtg gtgggcagcg tgtacgccct catcgccctc ggctacacca tggtctacgg
    14221 catcctcggc ctgatcaact tcgcccacgg cgacgtgctg atggtcggcg ccctggtggc
    14281 gctggaggcc atcgaactga tccaggcccg ctggccggac ctgggcgcga tccccacgct
    14341 gctcgccggc ctcggcgtgg ccaccgccac ctgcatggcc ctcggctaca ccatcgagcg
    14401 caccgcctac cgccgcctgc gcaatgcgcc gcggctggcg ccgctgatca ccgccatcgg
    14461 catgtccttc ctgctccaga ccgtcgccat gatcgtctgg ggccgcaact accacacctt
    14521 cccgcagctg gtctccacca caccgctgga acccgtcagc ggcgtcttca tcacgcccgt
    14581 gcagatcgtc atcctggcca cctcggcgct gctgatggtg gccctggtgc tgctggtgaa
    14641 caagacccgc ctcggccgcg ccatgcgtgc caccgccgag aaccaccggg tcgcaagcct
    14701 gatgggcgtg gataccaaca agatcatcgc ctccaccttc atcatcggtt ccggcctggc
    14761 cgccgtggcg ggggtgatgt tctcgtccaa ctacggcatc gcccattacg ccatgggctt
    14821 catgcccggc ctcaaggcct tcactgccgc agtgctgggg ggcatcggca acctgggcgg
    14881 cgccatgctc ggcggcatcg tcctgggcct ggtggaatcc atcggcgccg gctacatcga
    14941 ggacatgagc ttcggcttcc tcaactccag ctaccaggac atcttcgcct tcgccatcct
    15001 cggcatcgtg ctgatcttcc ggccgaccgg cctcctgggc gaacgcgtcg ccgaccgggc
    15061 ctgatgccat gaacgaactc gccctcgcca tcccctacct cggccgccgc caccccgccg
    15121 ccgcccgcgc ggtggtgctc ctgctggcct tcgccgcgcc gctcatcgcc attctgttcg
    15181 gcaagacctg ggtgcgcatc ctcgatttcg cgatgctcta cgtgatgctc gccatcggac
    15241 tcaacctggt ggtgggcttc gccggcctcc tcgacctggg ctacatcgcc ttttacgcgg
    15301 tgggggccta caccttcgcc ttcctggcct cgccccactt cgacctgcac ctgcccttct
    15361 ggctggtgct gcccctgggc gcagccttcg ccgccctggc cggcatcatc ctcggcctgc
    15421 ccaccctgcg cctgcgcggc gactatctgg ccatcgtcac tctgggtttc ggcgagatcg
    15481 tgcgcatctt cctcaacaac ctgaaccacc cggtgaacat caccaacggc cctcagggca
    15541 tcaacatgat cgacccgctg gtgctgttcg gctgggacat ctcacgcccg ctgaagatcg
    15601 gcgacgagat cacggtcaat tccctgttcc tgtactacta cgtcttcctc gcctgtgtgg
    15661 ccctgtcggt gatgctggtg cagcgtctgc aggtgtcccg ggtcggccgc gcctgggcgg
    15721 cgatccgcga cgacgagctg gcggccaagg ccatcggcat cgacacccgc aacatgaagc
    15781 tcctcgcctt ctccctcggc gccactttcg gcggcgtctc gggcgggctg tttgccgcct
    15841 tccagggctt cgtctcgccg gagagcttct cgctgatgga atccatcgcg gtgctgacca
    15901 tgatcgtctt tggcggcatg ggcaacctgg ccggcgtcat cgtcggcgcg ctggccctca
    15961 ccgccctgcc ggaagtcctc cgccacgtgg ccgtgcccct gcagcagtcc ctcttcgacc
    16021 gggtgatcct cgatcccgag gtgctgcgca tgctgctcct gtccctggcc atgatcttca
    16081 tgatgctgct gcgcccggcc ggcctgatcc ccgcccacag ccgctattcg cgccccgagc
    16141 tggtgctggg caaggagtcg gcgcggtgat caccctcctc gaagcccgcg gcatcaccaa
    16201 gcgcttcggc ggccttaccg ccctgtcgga cgtgtccctg accatccgca agggcgaggt
    16261 ctacggcctc atcggcccca acggcgctgg caagaccacc tttttcaacg tcctcaccgg
    16321 cgcctacacc gccgaagaag gcgagctggt cttcaacggc accgagctgc ccgccggcaa
    16381 gccccaccgc gtggtcgagg ccggcatcgc ccgcaccttc cagaacatcc gcctgttccg
    16441 cgacatgacc gccctcgaga acgtcatggc cggccaccac atccgctccc gcgccaaccc
    16501 tttcgccatc ctgctgcaga cccgccaggc ccgcgaggaa gaacgcctga tcaccgaacg
    16561 ggcctacgaa ctgctgcgct acgtgggcat cgagcgcttt gccgacaccg tgtcgaaaaa
    16621 cctctcctac ggcgaccagc gccgcctgga gatcgcccgc gccctcgcca ccgagcccca
    16681 gctcctcgcc ctcgacgaac cggccgccgg catgaacgcc accgagaccg gccgcctgcg
    16741 ggatctcgtc cagaccatcc gccacgacgg catcaccgtg ctcctcatcg agcacgacgt
    16801 gaagctcgtc atgggcctgt gcgaccgcct tgccgtgctc gacttcggcc gcaagatcgc
    16861 cgaggacgtg cccgccgagg tccagcgcaa cgaagccgtc atcgccgcct atctgggcgg
    16921 ccacagcacg gccgcggccg tgaaggaggc gtcatgagcg acgcgtccct cctcgaactg
    16981 accggcctgc gggtcgccta cggtgccatc gaggccgtca agggtatcga cctgcacctc
    17041 gccaagggcg agctggtggc cctgatcggc gccaacggcg ccggcaagac caccaccctc
    17101 aacgccatcg ccggcaccct gcccgccgcg ggcggcgaga tccgctacga cggcgcccgc
    17161 atcaacgccc ttcccgccca caggcgcctg cgcaagggcc tcgccctggt gccggaaggg
    17221 cgcggcatct tcacccgcct cacggtcgag gagaacctgc gcatgggcgc ctactgccgc
    17281 cgcgacgcgg ccggcgtgga agaggacatg gaccgtatct tcagcatgtt gccgcgggtc
    17341 aaggagcgcc tccagcaggt ggccggcacc ctctccggcg gcgagcagca gatggtcgcc
    17401 atcggccgcg ccctgctgtc gcgcccgcgc ctgctgttgt tggacgagcc ctccatgggc
    17461 ctcgcgcccc tggtggtgga aaagatcttc gaggtcatcc gctccgtcgc cgcgggcggc
    17521 gtcaccatcc tgctggtgga gcagaacgcc cacctggcgc tggaattctg ccagcgcgcc
    17581 tacgtgatgg aatccggcgc ggtcaccctg agcggcaccg gcgcccggct gctggccgac
    17641 cccaaggtcc gcgccgccta cctgggcgag ctctgagcgc ctgccgcgca gcccggcgga
    17701 ccctgtcgcg ccgggccaag gccggctagg ctgaagcatc cgcgacggag ttgcctccat
    17761 gtccgtccta tccgtcagcg cctcgtcgag ttccctgtcc gctacccgtc cgggcggcgc
    17821 ggggggcgcg tctgcgctga gcgacgccga acgccgcacc gtcgcccgaa tgcagcagcg
    17881 cgaccgggat gtacgcgccc acgagctggc ccacatcacc gccggtggcg gtttgatcac
    17941 ccgcggcgcc gcctacacct acgagaccgg cccggacggc cagcgctacg cggtgggcgg
    18001 cgatgtgggc atcgacgtgt cgccggggcg cacaccccag gaaaccctgg cccgcgccga
    18061 gcaggtccgg cgcgccgccc tcgccccggc ggacccctcg gcccaggatc gtcaggtcgc
    18121 cgcccgcgct gaccagatgg ccttccaggc ccagcaggcc atcgccgccc aggccacatc
    18181 tggcaccgat ccgaccagcg ataccccgcc gccgggcagc ctgattgacg tttacgcctg
    18241 atccgccgcc ccgcccggca ggtggcgcaa cagcccctcc atgcagcagg ccagcaccgc
    18301 cgcgttgccc agatgccagc cgaagtgcgt gccccacggc cagagtggac acagggcctg
    18361 atcgatgacc cggcacgcca ggctgaacag gaacaatccg ctcgccgcca tcagccaccg
    18421 accggctgcc aggtcacggc ggcgcgcctg ccacgcgcag tagcccagca gcgcaaaggg
    18481 cggcagatag atctccgaac cgttcaatga ccagggcggc ccccaccgca gcagcactgc
    18541 aaaacccgcc gccagcacca ggaacacccc cagcccgcgc cacgccgcag cgtccgtgcc
    18601 gcccagcaca tggcattgaa agcgctggaa atacgccagc acgaagacgg caatgaagcc
    18661 cacatccagc agcgccgccc aacgtgtggc caccgtgtgg aagaccaggc tgcccagccc
    18721 gaccatggcg atcagggcga tcaggccacg cgcatcccgc ggcgcggtcc gcccgtagcg
    18781 ccgcgccagc cacagggcca cggccacaaa tgccaggttg gtgagggcat tgagcggctc
    18841 cgacccgaaa ctggcgtcgg tgcgctcgca atagaggtcg atactttcgg cccagttcat
    18901 gaaacgcctc ggcggggacg ggaaatcggc tcgccattat gccgtcggtg cgtgcgcgac
    18961 gacggtcggt gtcggtcggc tcaatgcacg gtgggacggc agtgcgccgc tggcgatgca
    19021 ggatcaagtc gattcggctt gaaatgtgca attcacacat ttaatataag ctcatattcg
    19081 gcagttcatt cgtctggccg ggtcattgca ggcggagcct gcacggggcg ttccgacgga
    19141 acgccgttcg ttatcgatct gaatgggaca tctccatgca tactccgcac gcaacgcgcc
    19201 gcctgccacg cctgggcctt gccctggccg tcgcggtgcc cctggtgctc ggcggcctcg
    19261 cctttgccga cggcagtctc ctcaagtcgc cgcagggcaa gggcgaaacg gaacgcaacg
    19321 ccagcggcac cggcacgccg gctgccacca cgccgacgcg caaccgcgcg tcatccggtg
    19381 aagacggcgg gcgcgtcacg ccctcgtcga gcagtagcgg aagcggcgtg acgacggtgt
    19441 ccatcgtcgg cagcccgacc gtcgccaccg gcagccagac cgtcgtgacc agcacggcgg
    19501 cggccaccct gccgcgttac accctgctgg cctggaacga tctgggcatg cactgcgtgg
    19561 acggcaagga ctactccatc atgtccatcc tgccgcccta caacaacctg cacgcccagc
    19621 tcatcgaccg gcagagcggc aaacaggtta cctccggcgt caccctgagc tacgaatccc
    19681 tgcgcgatcc gtccggctcc atcaacacct tctcggccgg caagaccaat ttctggcagt
    19741 acgtgaaggc cttgtacggg ggctcgccgg cgccggaccg gggtctcaac ctgagcaatc
    19801 ccgccgtctc caatcccacg cccagcagcc gcccggcggc catgagcttc aacacggtgt
    19861 ggcgctggta cgaagcggag ggcctgccca tcctgcccta cgacgacacc ctgacgaccg
    19921 ccggctacac caagaattac tacccgatgg tcaaggtggt ggcgaagaac agcactggca
    19981 cggtgctcgc cagcacccag accgtgctgc cggtctccga cgagatgacc tgcgtcggct
    20041 gccacgccag caattccaac cgggacgccg ccaggccacc cgtgcggggc tgggccaact
    20101 tcaccgccaa cccggaaaag gactggaaga agaacgtcct gcgcctgcac gacgacaaac
    20161 acgccaacga cccggtctac caggcggccc tgctgaagct ccagcccgcc gccagcggcg
    20221 gcctggccct gcgcgccgac cagggcacgc cgaccctgtg ctcgtcctgt cacggctcca
    20281 acgcgcttaa ttcgccgggc ttcaccgtga ccgtgaatgg ccgcgccgtg aagatctccg
    20341 ccttcacctc ggccatgcac accctgcacg gcaaggtgaa ggacccggtc aagcaggtgg
    20401 ccctcgacac gattggcaac cgggattcct gttaccagtg ccacccgggc gccgtcacca
    20461 agtgcctgcg cggggccatg agcgacacac cgaacctgga ctgccagagc tgccacggca
    20521 acatgggcgc cgtcggcaaa tccacccgag tgggctggct gcagcaaccc aactgccagt
    20581 cctgtcacca tgacggcaaa cgcgagacgg tggctgtcac cgatgtcacc accggcgccc
    20641 tgcgcgcggt gaccgacacg cgcttcgcca ccgagcccaa caagccggcc accggctatt
    20701 ccctgttccg cttcagcgtc ggccacggca agaaccagtg cgaagcctgc cacggctcca
    20761 cccacgcgga gtattcgtcc aagaatcagg cctgggcctc gtcccacgac aacgacctgc
    20821 tccagtccaa ggcgatccag ggctacgccg gcaccctcac cgaatgcagc gtgtgccaca
    20881 ccagcgtgcc cctcaccacc aatggcggcc cccatggcct gcatacgatt gggcaggcct
    20941 gggtgaatgc ccacggcgac cgggtcgaat ccagcggcgc cagcgcctgc aagagctgcc
    21001 acggcgcgga ctaccggggc accgccctgt cccaggtgaa agtcgccaag accttcagcc
    21061 acgacggcag gcaatacgcg ctggcggccg gcacccaggt cggctgttac acctgccaca
    21121 acggaccgaa gggcgactga ccgcaaagtc caccgtccag gccgggggcg gcagcgtccc
    21181 cggcccgatc tgggggatcc ggcgggagcc ggaaagccgt gccgtgcctg cgctcggggc
    21241 gcaatgggtc ataatgagaa cgattctccg gaccgcaggt ccaccgttct cccagcttga
    21301 aatgtgcaga tcgcacattc acaatgtccc ctgcaatgat ccccaaggcg gtgtcatgac
    21361 gcccccctcc gagccccacg gtcctgccgg cggcgagggc ccatcgtccc tcgatccggc
    21421 cctggccgat gccctgcgcg ccgtgctggc gcccctggca cgcttggcgc tcgcccgcgg
    21481 cgtgtcctgc ggggccgtgg aagaactgct caaggagatg ttcgtggacg aagccatgcg
    21541 cgcccaaggc gcgtcggcat ccgggcggat ggtcagtcgc atcagtgtgg ccaccggcct
    21601 caaccggcgc gaggtgacac gtctgacccg cgccgagcgg gcccctcgcg ccgtccgcca
    21661 accgccgagc tcggaagtct tcgcccgctg gatcaccgac ccggcgctgc gcgatgcaga
    21721 cggccggccc gtcgagcttc cccgcctgcc cgacgaacgc ggcagcccca gtttcgaaac
    21781 cctggcccgc tcggtcaccc gcgacgtcca tccgcgcagt ctgctggacg aactgtgccg
    21841 catcggggtc gcccgccacg acgaggcgac cgaccgggtc tgcctgctgc gtgccgactt
    21901 cgtacccgat gcggaccggg gcggcatgct cggcttcctg ggcgacaacg tgggcgatca
    21961 cctgaatgcg gcggtggcca acgtgaccgc cgccgtgccg attcacttcg aacaggcggt
    22021 gtttgccgac gagctgtcgg agacggccct cgtcgcggtc aaggccttgg tccgggacca
    22081 ctggctggcg ctggtccacg gcgccgtgcc ggccctggaa gccctcatcg aggctgaccg
    22141 ccaggccggc cgcgcccagg accggcgggt ccgcatcggc atgtacagct acgccgcacc
    22201 acagaccgcg ccggttggcc cggcggaaga atcgacacca tgacgacttt tctcacccgt
    22261 gccggacggc acttctgcgc gctgctggtc attctgttcg cgctggcctc ctgcgggggc
    22321 ggcggcagcg gcgtcggcga tctggccggc gtgggcaccg gcggcacggg tccggccgcc
    22381 gtggggatcg gaccgatcag cggcttcggc tcggtgatcg tcggcggtgt gcgctacgac
    22441 gacagtcgtg cggtcgtcac cgacgatgag ggcgcgcccc gcagtcgcct ggacctgcgc
    22501 ctcgggatgg tcgccgagat cagcggcagc gccgatttca ccgcgcgcac cggcgtggcc
    22561 acggccatcc gctacggcag cgaagtactc gggccggtga cctcagtgaa taccgcggcg
    22621 aatcgcttca gcgtcctggg cgtcggcgtg gacgtcgtgg ccgacaccgt attcgacgag
    22681 cgcctgaccg gcgtggcagc gctggggatg ggcgacccgg tggaggtgta tggctattac
    22741 aacgtggcca ccggccgctt caccgccacc cgcgtggaca gtgccaccgg agcgtcccgc
    22801 tacacactgc ggggcaccgt gagcgggctc gacacgacgc gcaagcgctt tgcgctggcc
    22861 ggtctgacga tcgactacgc cgctgttccg gcggcgcaga ccgcggccct ggccaacgga
    22921 caggccctgc gggtgagtgc gacgcgcgcc cccgcgggcg gcgtgtgggt catcgacgcc
    22981 ctgacgggcg ccgcccgggc cagtctggca gagggcgagg ccgagatcgt cgggcgcatc
    23041 gacagcctga cctcggcacg acacttcagc gtggagggcg tgagcgtgga tgccacggcg
    23101 gcgagcgtca gtggtacgcc ggccctcggc ctgcgcgtgc aggtggaagg caccgtgaag
    23161 aacggcgtcc ttcaggccag ggaagtggag gtgtcggacg acggccagag cgagacggag
    23221 gaattcgacc tgaccgacgt gatccagtcc ttcgacccgg ccacccagcg tttccgcctg
    23281 cgtggccagc tcgtggatgc gagcggaacc gtaagcttcg aagacggcag ccgcagcgac
    23341 ctggccaacg gcgtgcgcat cgagtgcaag ggcagcttcg atgccgcgct gggcgccgtg
    23401 cgggcgcgtc tcatccactt cgagcgctga agtggagcgg ccacctcagg cgccgggctt
    23461 cggggcctga agccgcgtcc acagcgccac ccccaccagc cccagggcca tgaagccgag
    23521 catgccgaag gccaggcccg gcacggtccg ccacagggcg ggcgcgacca ggctggccga
    23581 taccgcgctg gtcatcatct gcaggaagct ctggcagctc gccgccaggc cccggttggc
    23641 cggaaagtac tccagcgcca gcagggtcag gctgggcatg gccacggcca tgccgaagtt
    23701 gtagaccatc accggcagca ccgagaccgg gatgcccggc gccaggagca ggctcacgcc
    23761 gacgttcaag gcacatgcga gggccatcac ggcgtagccg agagccaccg tgcgggtctg
    23821 gctgaggcgc ccggcgagcc gtgccgagag ggccgaaccg ctcatcatgc ccaccacgcc
    23881 ggggatgaag agccagccga actcccgctc cgacaggccc aggtgagtca tcaggaacac
    23941 cggcgccgac agcacgtaga tgaagaatcc attgaagttg aaccccaccg ccagagtcaa
    24001 cagcaggaaa ccggcatgac ggaaggcgtc ccggtagctg cgcgcgaggc tggccggatg
    24061 cagcggacga cgctcggacg gggcgagggt ttccggcagg ccgacggcgg tgagcagggc
    24121 cagcaccagg ccgagcacgg agagaaagac gaacaccgcg cgccagccga acaggctgaa
    24181 gatccagcca ccgaggatgg gcgccagggc cggaccgagg gcaaaggcca tcgccacgtg
    24241 gctcatcagg cgctgggcgc gggggccttc gtgcagatcg cgcaccacgg cgcggccgat
    24301 caccatgccg gcgccagccg tgatcccctg cagcgcccgt ccggccatca gcatctccag
    24361 gctgctggcg aagacgcaca ccagcgacgc cagcccgaac agcagcatgg aaccgacgat
    24421 caccggcttg cgcccccagg catccgacag ggctccatgc cacagcacca tcgcgcccat
    24481 gggcagcatg taggcggtga gggtgagctg gacctgctcc ggcgtggcgt ggagttccgc
    24541 cccgatggcg tgaaaggccg gcaggtaggt gtcgatggag aaagggccga cggtggcgag
    24601 ggctgcgagc agcgccgcca gctggacgta gggcatggaa gaccttgcgg ggcgcgccgg
    24661 ggcccgggcg cgcgacggga gccggcttac tcgacggcgt gaacctggct cacgaaatag
    24721 ccggtctcac cgaagcaggt ggtggggatg cccacgtgct gatcgtaggt cagggacacg
    24781 cgcttgccca tcaccgcgtt gatgtcggtg gcgaccttct cgtcgcggac cgtgaagcgg
    24841 aagatctcgg gcaggctgcc gggaatggcg acgatggcca gctcgccttc ccaggttttg
    24901 cacagccagc ccttcttgga gaacttctgc acgtagccgg cgcgttcgcc gttggagtaa
    24961 ctccagctga gcacgagcca ggtgtaagcc gccacaaggg cggcaacgat cacaaaaaat
    25021 ccgatcagcg agaccagcca tttcttgctg ctgcgcggtg cagccggtgc gggggagacg
    25081 ctcactttca gctccaaggg ggatcagacg gttccgggca gtccgcggcg gctctgcacc
    25141 gcttcggcca cgtgggcggc acccacgtcg gggcggcccg ccaggtcggc gattgtacgc
    25201 gcgacccgca agatgcgatg gtacccccgc gcggacaagc cgagccgggc gatggcggtc
    25261 tgtaacagat tcacgccggc ggcgtccggc cggcaccagc ggtccacctc ggccccgccc
    25321 aggtcggcat tggccttgcc ctgtcgggcc tgctgcacgg cccgtgcggt ggcgacccgc
    25381 tcgcgcacgg cggcagtggg ctcgccggcc ggtgcggcct gcaggtcggc ggccggcagg
    25441 gcggggactt ccaggagcag gtcgatgcgg tccagcaagg ggccggagag cttgccgcga
    25501 tagcgcgcga cctggtccgg cgtgcaccgg cagcggccgt cggggtgccc caggtagccg
    25561 caggggcagg gattcatcgc cgccacgagc tggaagcggg cgggaaagtc cgcccgccgc
    25621 gcggcgcggg agacggtgat gtggccgttc tcgaggggct cgcggagggc ttcgaggacg
    25681 cgccggtcga attcgggcag ctcgtcgagg aacagcacgc cctggtgggc gaggctgatc
    25741 tcgccggggc gcggggtact gccgccgccg acaatggcgg ccaccgaggc gccgtgatgg
    25801 ggactgcgcc acggccgctg gcgccagcgc tcgggccgga agctgccatc caccgactgg
    25861 atcgcggcgc tctccagcgc ctcgacgtcg tccatgggcg gcagcaggcc gggcaggcgc
    25921 tgggccagca tggatttgcc ggtgcccggc ggaccgaaca ggagcaggga atggcgcccg
    25981 gcggcggcca cttcgagggc gcgacgcgca ggtgcctggc ctttcacgtc ggccaggtcg
    26041 gggtagctga cctcggccag cagcggcgcg ccggcgtagg gcgccagcgg cgtgtggccg
    26101 cacaggtgcg ccgccacgtc cagcagactg gccgccggca gcaggacaag gccgtccacc
    26161 agggccgcct cggcggcgtt ggcagtgggc aggatcaggc gccgaccgct gcccgcggcc
    26221 tgacgggcca gggccagagc gccacgcacc gggcgcagtt cgccggtaag ggacagctcg
    26281 ccggcgaatt cgtacccggc cagggcctcg gcggggatct ggccggaggc ggcgaggatg
    26341 cccagggcaa tgggcaggtc gaagcagccg ccttccttgg gcagatcggc gggggccaga
    26401 ttgacggtga tgcgccgctg gggaaattcg aactggctgg tctgcagcgc ggcgcggacc
    26461 cggtcgcggg cctcgcgcac ctccatgtcg ggcaggccga ccagggtgaa ggcgggcagg
    26521 ccgttggcca ggtgcacctc gacgatcacc tcgggggcct gcatgccggc cagggcgcgg
    26581 ctcttcagga gtgccaggga catgatccgg atcgcatgca aagccgtgag tgtagcctgt
    26641 acggacgtac cgtgggatgc ccgtcgcatg gtttcagcgg ccgaccggca gggtgagctg
    26701 gggaccgggg ccggtcttgc cggcgagctc caggcgggcc agctccatca gcagggagaa
    26761 ggtggatttg ctgtcggtgt cccgatcatc cacgtaaaac cagtggtcgc ggtagcgcac
    26821 cgccacgtgg gcgtccgccg gacgggcgtc ggcggcgctg gcgcggacgc ggaacaggcc
    26881 gatcatcacc cgctgccagt cgaagggcgt gccgtccgcg tcgcgggtga gggtgacgac
    26941 gccccgttcc agatgcgcgg gcggcacgtc tacgccgagg ctcacgtaat acagggcctg
    27001 gagcagggat cgggtttcca ggtcaatctg ggtgaggcca ccgcggggct gctgttcccg
    27061 gaagggtggc agcgcctcct gggtgacggc gtactgcagg aggcccggct tgagccggaa
    27121 agcactcagg aagcgctgcg tcgcctccgc gttcacggcc gacggggcga tctgcagggt
    27181 cgggcgggtg aatctgcgga ccaggctcca gctgcggccg tcctcatcgg ggcggtacac
    27241 gtatcccgag cgggccgcct cgatcacgtc gcgaggcgtc accgcggtgg cgggcagcgg
    27301 gccgccgagc agttcttcct gttcttccac gccgaagacg acgtcgcccc gatcctgcag
    27361 acgctgcaag gcgtgcacgc cctcgagaaa gtcgttgatg gggggcgcct ggcgcggcgt
    27421 ggggccgctg gccgtctggg cgttgggcac ccagttgagg ttttccagat agaggcggaa
    27481 caccgtggag atgggccagg tggtcttggc caggtagacc agaccttcaa ggggaagggg
    27541 cgtaaacagc ttgcgcgtga actcctggtc gtccagaggc gtgaagctga aggtgggccg
    27601 gtcggcgcgg gtggcctggg cctggggcag catggcggtg aagctgcggt tgaggtcacc
    27661 gccggcggcc gtgaagaagg gcagcagtcc gaggctctgg gccacctcga actgggcggc
    27721 gatgccggtc acggcaaggc tgctgggcgt gtcggtgtag cgcagtcgga cgatgttgag
    27781 gaggagctgc tcctccgcgg tgcgcttgag ggcttcgttg tactgcaggc gggtctggtc
    27841 gatggccggc ggaccgatgc cggcacaggc ggccagcagt ggcacggaca tgcaggcggc
    27901 caggaggtgg cggacagacc ggctggagcg gaacgcgggg atcatggcac gagtgtgccg
    27961 ccccaggatg acggggccgt gaccgggacc gcccatcctc cgggaaggcc gggcaccatg
    28021 cccggcggga ttcagtcgcg gaaggcttcg acggcgatga tcaggcgcac ttcgtcgctg
    28081 acggacggaa tgttgcgggt catgccgaac tcggaacgct tcagccggcc ggtgatgtcg
    28141 gcgccacagg tctcccgctt gttgagcggg ttgggcgcac agtggacgcg ctgcagcgtg
    28201 agcgtgaggg gacgcgacac acccagcagg gtcaccgtgc cctcggctgc caccggcgtg
    28261 tcgccgtcaa acaccaggcg cgtggaccgg aagctcacgg cgggaaactc ggcgacgttg
    28321 aagaagttct cgccgcgcat gttctcgttc cacttggcga agcccatgtc cagggccgcc
    28381 gcatcgagac tgacatccac cgtgccgctg cgggcggccc ggtcgagcac gatcttgccg
    28441 ctgctgcggc tgaagcggcc acgctgggtg gacaggccca ggtggtcggt ctcgaagatc
    28501 acgtgggtgt ggttcggatc gatgctgtag gacaccggct ccgcccgcac gggcagaacc
    28561 ccgccgacgg tcaggagcgc agccagcgca caacgcaggg ccgacatgtt tcagacgcct
    28621 tcggcaggcg gacgcttggc cagctcggcc tccaggtggg cgacccggtg ttcgagttcg
    28681 gcaagctttt cgcgggtacg cgccagcacg tcgcgctgga cctcgaattc ctcgcgggtc
    28741 accagatcaa gcttggaaaa gccgctggcg agcagggcac gggcattttt ctccagatcg
    28801 cgggccggac tgttggcaag cagctcagac actttggagc cgatttcgtc gaggatcttc
    28861 ggattggcca tggcaggact cctgtacacg atagagggca ttccgggcga cccggagcaa
    28921 ggtccgagtg tagcaccggg tctgcgcgcc gcgacggcat gcccatgcac ccgaatggcg
    28981 caccgacttg gtgcaacatt ccaacttggt gcgcacgata aaaattgcgc accgcaatag
    29041 gtcacgaaaa acaagtttaa cttactgttt acaaaagaat taatgttttt ggcacatgct
    29101 ttgctataca gttctgccaa ctttgcaaac aggagattcc taccatgcgc aagaccgtcc
    29161 ttgcttccgc cctgctcgct tcctttgtgg ccgcccccgc cgtcagcttt gccgaagacg
    29221 ccgcgcctgc cgcgcccgcc ttcaccagca acgtgaccct ggcgtccgag tacatctacc
    29281 gcggcatcgg ccagaccaac cgcaagcccg ccatccaggg cggcttcgac tacgcccatt
    29341 ccagcggcct gtacgtcggc acctgggcgt ccaacnnnnn nnnnnnnnnn nnnnnnnnnn
    29401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnnnnggcaa cggccgcgtg ctgttctccg tgacccgcac gttctaattt
    29521 cgaactggca tggcggcccg gtgacgggtc gcccagcatg aggaataccg tcatgaaact
    29581 cgttaccgcc atcatcaagc ccttcaagct cgacgaagtg cgtgaggctc tgtccgccat
    29641 cggcgtgcaa ggcatcacgg tcaccgaggt caagggtttc ggccggcaga aaggtcacac
    29701 cgagctgtac cggggcgccg aatacgtcgt cgacttcctg cccaaggtga agatcgaagc
    29761 cgccgtgcag accgaaatcc tcgaccaggt gatcgaagcc atcgaaaagt ccgccagcac
    29821 cggcaagatc ggtgacggca agatcttcgt gttcgatctg gaacaggcca tccgcatccg
    29881 taccggcgag tccggtaacg aagcgctgta agggagcgtt cttatcatga aaaagttcgt
    29941 agctgtcctg ctgaccgcct ttgcggtcaa ttttgcgggc ggcgcgctgg cggatgacaa
    30001 gcccgccgcg ccagccgcag ccgtcacggc acccgccgag gccaagccgg ccgaagcccc
    30061 cgccgccgaa gccaagcccg ccgaagcggc ccccgctgcc gctgccgaag ccgccccggc
    30121 cgaagccgct gccgcgccgg tgccccccaa caagggcgac aacgcctggc tgatcgtcgc
    30181 caccgccttc gtcatcctga tgtccatccc tggcctggcc ctgttctacg gcggcctggt
    30241 ccgtgccaag aacatgctgt cggtgctcct gcaggtgttc acggtgttct cgctgatcag
    30301 cgtcctgtgg gtgctgtacg gctactccgt ggccttcacc gagggcaacg ccttcttcgg
    30361 cggtctggac aaggtcttcc tgaagggcat cacgccggat tccgtggcgg ccaccttcag
    30421 caaggccacc aacatctccg agttcatcta cgtggccttc cagggcgcct tcgcggcgat
    30481 caccgtggcc ctgatcctcg gcgccttcgc cgagcgcatc aagttctcgg ccgtgctgct
    30541 gttctccgtg ctgtggttca ccttcagcta cctgcccatc gcccacatgg tgtggttctg
    30601 ggccggtccg gacgcctaca cctccgccga agcggccgat gcggccaacg cgacggccgg
    30661 ctggctgttc cagaagggcg ccctcgactt cgccggcggc accgtggtgc acatcaacgc
    30721 cgccgtggcc ggcctggtgg gtgccttcct ggtgggcaag cggatcggct tcggtcgtga
    30781 atccttcgcg ccccacagcc tgaccctgac catcgtcggc gcctccctgc tgtgggtggg
    30841 ctggttcggc ttcaacgccg gttccgccct ggaagccaac ggcgtcgccg ccctggcctt
    30901 cgtgaatacc tgggttgcca ccgctgccgc cgccatgtcc tggctgctgg ccgaatggat
    30961 catcaagggc aagccctctg gcctgggcgc tgcttccggt gccgtggccg gcctcgtggc
    31021 catcaccccc gcctgcggct tcgtcggtgt ggtcggtgcc atcgtgatcg gtctgcttgc
    31081 cggcgtggtc tgcctgtggg gtgtcaatgg tctcaagcgt atgctcggcg ccgacgactc
    31141 cctggacgtc ttcggagtgc acggcgtggg cggcatcctc ggcgccaccc tgaccggcgt
    31201 gttcgcggcc ccctccctgg gcggctccgg catttgggac tacgtggcca acaaggccgc
    31261 cgacgcctac tccatcggtg accaggtcgt gatccagctg accgccgtcg gcaccaccat
    31321 cgtgtggtcc gccgtggtgg ccttcatctc ctacaagctg gtcgacatgt tcatcggtct
    31381 gcgcgtgccg gaagacgaag agcgcgaagg tctggacgtc acctcccacg gcgaaaccgc
    31441 ttaccatcag taacatcggt aacaggttcc gtcggcaaaa cgggcgcttc ggcgcccgtt
    31501 ttcatttgtt cggcgcgcct gggcagacgc acgcgcccgg gtccattcca gacacgcgct
    31561 ccatggacgc accggataaa tgccatcggc gtacaatggt gcgatgttcc gttcccccgc
    31621 cagatcgcct cgctgggcgg ctgtcatcgt cgcgctggtc ctgctggttc cgcccctgca
    31681 cgcctcggcg cgggaggcgg tgaccctgca gctcaaatgg cgtcacgcct tccagtttgc
    31741 cggctactac gcggccaagg aaaaggggtt ctacgacgcc gccggcctgg acgtgcatct
    31801 gcaggaagcc acacccggca tggatccggt cgagccggtc ctcaacggtc aggcccagta
    31861 tggcgtgggc aacagcagcc tgttgctggc acgccgggcc ggtcagccgg tggtcgtgct
    31921 ggccgtggtg ttccagcatt cacccctggt gctgctgacg cgacagcaac ccgacacggc
    31981 agacgcgcaa ggccttcagc acctcgccgg caaacgggtg atgatcgaac cccattccga
    32041 cgaactgatc gcctatctga aacgggaggg tgttcccctc gaccagctgg tccaggtgcc
    32101 ccacagctac cggcccgagg acctgatcga aggccgggtc gatgccatca cggcctacgc
    32161 cagcaatgag cccttctacc tcgaccgcgc cggcctcgcc taccaatcct attcgccccg
    32221 ggccgccggc atcgacttct acggcgacaa cctcttcacc accgaagacg aattgcgcaa
    32281 ccacccggat cgggtccgcg ccttccggga cgccagcctg agaggctggc attacgccct
    32341 ggagcacccg accgagattg ccgacctgct ccaccgccac tacgcgcccc agcacccgcg
    32401 agccttcttt cttttcgaag cccagcgcat gatgccgctg ctgcgcgccg acctcatcga
    32461 ggtgggctac atgaatccgg agcgctggcg ccacattgcc gacacctacg ccgatctggg
    32521 cctgctgccc cgcgactacg cccttgacgg cttcctctac gcaccggacg tccagctcga
    32581 ccgaagccgc ttctacctga ccctggcctt gctggcgctg gtcacgggcg ccgccatgta
    32641 cgtggtccgc atcaaccggc gcctgtcccg cgtgctggcc gaaagtcagg ccgccgaagc
    32701 ccgcatccgg cacctggccc agcacgaccc actgaccgag ctgcccaatc gtgccctcct
    32761 gtccgaccgc ctgggccagg ccctggtggc cgccaaacgc gacacccggc gcctggcggt
    32821 gatgttcatc gacgtggaca acttcaaacc catcaacgac accctggggc acgcggtggg
    32881 cgatcgcctg ttacagcaga tcgccaagcg cctgcaggcc agcctgcggg aatcggacac
    32941 cgtggcccgc atcggtggag atgaattcgt ggtgctcctg cgcaacgtca cggacgggcc
    33001 ggacgccctc gccgtggcgg acaaactccg cgaagtcctc tccgtaccca ttgtggtgga
    33061 ggcacacacc ctcgtcgtcg gtgccagcgt cggcgtggcc ctgtaccccg accacggcag
    33121 cgatgcgctc gaactgttcc ggcacgccga cgaagccatg tatcgcgcca aacgcgacgg
    33181 tcgcaaccgg gcacaggtgt tcgaagcccg tagcgaccgg gggcagcccc tggcgccgtc
    33241 gcagcctgcg gcttcgagcg tcggcccgta aacgtgcaac gggcgcccca ccgggcgccc
    33301 gccatccagc cgaacgccgg actgcgccgc atgccgcggc gcgccggtcg tccaggcccc
    33361 gctgctcacg cgtgggcctc ctccccgtcc agcggatcga gcaactgacc acgcatcagc
    33421 gcaatcgggc tgcgccagta gatgtcgatg ttgtggaacg gatcggtcag gatcttgaaa
    33481 gcccaggcga gtccccagac cggtccgcgc aggaagaaca ggtgcagggt gcggaagccc
    33541 agcccgccgg caccgaggca cagccacagc agacccaggt tgtggaggta gccggccgcg
    33601 ccgccgcccg cgtcgagcag cccgaaggcg tcgggactca ccagcagcag caccgggatg
    33661 gacagccaca cgccgatcag cacgcccttg cgctgcatgt tgtaacccac cttgatggat
    33721 tccttgtgcg cgtaggtggt gttgtggatg gcgtcgaagc cgcgcggctc gtagaagatg
    33781 tggccggact ggcgcgtcac catgcccacc agccagccca ccagcccggc cagggccggg
    33841 tcggagaaca gcaggccgta ggccacgaga aaactgatcg cgctgatcag gtgcaaggtc
    33901 tggttgatgc ggctctggtg gtaatagcgg tggtcgtccc agcggagggt ccgcaccttg
    33961 tcgagaaagt cacgcacgtt gaaggctccc ggtaatgaat gctttgcgtg accgcaatgt
    34021 gacgggcttg catgacgctc cggtaatgga ggcgttacag atttgcgtca cggagaagtc
    34081 gtttgagcca ggtcaacatt tcgggacgca cgcgccacgt caccgtcaca tcgggcgccg
    34141 cggccatgaa aaagccggcc acgcgggccg gctcacacgg gtctgacggc ggacggctca
    34201 gcggaacacg accgtcttgt tgccgtggac cagcacccgg tcttccaggt gataacgcag
    34261 gccgcgggcg aggaccgcct tctcgatatc cttgccgtaa cggaccatgt cctccaccga
    34321 gtcggaatgg tcgatgcgga tcacgtcctg ctcgatgatg gggccctggt ccaggtcggc
    34381 ggtgacgtaa tggcaggtgg cgccgatcag cttgacgccc cggtcgtagg cttggtgata
    34441 gggcttggcg ccgacgaagc tgggcaggaa gctgtggtgg atgttgatga tccggcccgg
    34501 atgggcggcg cacaggtccg ggctgaggat ctgcatgtaa cgcgccagca ccatggtgtc
    34561 gccgtggact tcctcgaaga tgcgctgcac ctcggcgaaa gcgcg
//
LOCUS       DAOQMM010000028        33877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_338_length_33877_cov_49.859677, whole genome shotgun sequence.
ACCESSION   DAOQMM010000028 DAOQMM010000000
VERSION     DAOQMM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 33877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33877
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_33877_cov_49.859677"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..456)
                     /locus_tag="PLH85_06875"
     CDS             complement(1..456)
                     /locus_tag="PLH85_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNB64320.1"
                     /translation="MRLLPLSLALVAVVSVTGCASGLGGGTYERAEARRVMNVRMGVI
                     ESVRVVKLEGTKSPIGTLGGAAVGGIAGSTVGGGKGSSIGAVVGAVVGGLAGSAAEEG
                     LTRKNGLEITLKMDNGEMLAIVQEDEGEGFKPGERVRLLQDGRTTRVTR"
     gene            complement(468..1403)
                     /locus_tag="PLH85_06880"
     CDS             complement(468..1403)
                     /locus_tag="PLH85_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNB64321.1"
                     /translation="MSTLICGSIAFDSIMVFHDRFRNHILPEQIHILNVSFLVPEMRR
                     EFGGCAGNIAYNLKLLGGDPLIMATVGDDAGPYLERLDGLGLSRAHVTHVPGTYTAQA
                     FITTDLDDNQITAFHPGAMTQSHLNKVSDARDVRLGIVAPDGRDGMLEHCRQFAEAGI
                     PFIFDPGQGLPMFSGEELVACLQLADYCTVNDYEARLMCERTGRTPASLSGELKALIV
                     TRGGEGSEIHTGGQRIDIPCVRPAAVEDPTGCGDAYRAGLLYGIEAGLDWALTGRLAS
                     LMGALKIASRGGQNHTPRRDEIAERFREAFGTTLW"
     gene            complement(1487..2794)
                     /locus_tag="PLH85_06885"
     CDS             complement(1487..2794)
                     /locus_tag="PLH85_06885"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HNB64322.1"
                     /translation="MILARCPACATTFRVRPEQLRARQGRVRCGHCQHAFNALETLVE
                     NPVPPPPASVDGEPLAEAVESPVFILEEKSVRARHDWTAPDPDSPSPHIEPIGEIPPL
                     PDDAPAEPELLDAADEPTSQTPAFDAAPLADPFPDGSTHTLPERLDDAPPVEPTFILD
                     DPPARHGDGPPDEHLLPLDVDEHVAEIARATEPAPIESARDDGASTEPPPAAAFPLPP
                     DFGAPPNDVRLEPEPGAAPDPAPKPFTLVADADDAPFVLPAPQAGPDTSAADDEDAPP
                     RGLRQAVWAAAVTFLIVALLAQGVLVFRNRIALAAPALRPALEALCDRIGCDMPLPRD
                     SSLITIESSDMQPDGSREARFTLYATLRNRADFEQAYPYLEITLTDARDKALVRRVLE
                     PAQWLPPDARKAAFAPNQEISARIVFDAPGVAAAGYQVLAFHP"
     gene            complement(2791..3693)
                     /gene="prmA"
                     /locus_tag="PLH85_06890"
     CDS             complement(2791..3693)
                     /gene="prmA"
                     /locus_tag="PLH85_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNB64323.1"
                     /translation="MWLSVILQADAARAETLSEALMEHGALSVSIDDADAGTEAERPQ
                     FGEPGMHPASLWDHSRVVALFDPDADLASALSAAAGEAGLDAVPPFHIEEVEEQNWVQ
                     LTQSQFDPIRVTDRLWIVPSWHAAPDPDAINLVLDPGMAFGTGSHPTTRLCLEWLATS
                     LQPGQSVLDYGCGSGILGIAAARLGAGTVTGVDIDEKAVETAADNARNNGVHLTLLHS
                     STALDGQFDRVVANILTNPLKMLAPLLASHIAPGGRIALSGVLEAQADQVIAAYAPYI
                     ALRVADLSDGWVRLEGVRPEQTGP"
     gene            complement(3698..5059)
                     /gene="accC"
                     /locus_tag="PLH85_06895"
     CDS             complement(3698..5059)
                     /gene="accC"
                     /locus_tag="PLH85_06895"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB64324.1"
                     /translation="MFEKILIANRGEIALRVLRACRELGIKTVAVHSEPDADAKYVRL
                     ADESVCIGPASSALSYLNIPALISAAEVTDAEAIHPGYGFLSENADFAERVEQSGFVF
                     IGPRAETIRLMGDKVSAKDAMKAAGVPCVPGSEGALPEDPKEIVKIARSIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVTTTRAEAQAAFGNPVVYMEKFLENPRHVEIQVLADQ
                     HGNAIYLGERDCSMQRRHQKVIEEAPAPGIERRHITRIGERCAEACRKIGYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTELITGVDIVQEQILVAAGQKLRYRQKDIVLRGHA
                     IECRINAEDPFKFTPCPGRITHWHVPGGPGIRVDSHVYNGYTVPQHYDSMIGKLIAYG
                     DTRDQAIRRMRIALSEMMVEGIKTNVALHQDLMRDAKFIEGGTSIHYLEHKLAARNEV
                     KQG"
     gene            complement(5071..5526)
                     /gene="accB"
                     /locus_tag="PLH85_06900"
     CDS             complement(5071..5526)
                     /gene="accB"
                     /locus_tag="PLH85_06900"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNB64325.1"
                     /translation="MDLRKLKKLIDLVQESGISELEVTEGEEKVRIAKHSAGPAPVAY
                     AAPAPMAINVPAPAAAPAAAAPAAEPALPDGHVVKSPMVGTFYRASAPGAKPIVEVGQ
                     SIAAGEALCIIEAMKLMNEIEADVAGVVKAILVENGEPVEYGEPLFIIG"
     gene            complement(5581..6081)
                     /gene="aroQ"
                     /locus_tag="PLH85_06905"
     CDS             complement(5581..6081)
                     /gene="aroQ"
                     /locus_tag="PLH85_06905"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNB64326.1"
                     /translation="MTRKNRTQEGTVGELPQTRILVLHGPNLNLLGRREPGIYGLTTL
                     ADIHGAMESRARADGVCLESFQSNHEGELIDRVQAAESEGVAFIIINPAGYTHTSVAL
                     RDALTGVAIPFVEVHLSNIHAREPFRHHSYFSDKAVGVICGLGAQGYQLALEFVLARL
                     RTAPGA"
     gene            complement(6143..6694)
                     /locus_tag="PLH85_06910"
     CDS             complement(6143..6694)
                     /locus_tag="PLH85_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB64327.1"
                     /translation="MTRLTTWSAIAALGLLAAAAGLLTRQHVTDPPSPAPTAAARDAT
                     ARLMALSLPDSAGTQQALQQWRGQVLVVNFWATWCPPCRKEMPEFATLSDKYKGRKVQ
                     FVGISIDSPKNVAEFAAKSPVPYPLLIASPDVITLTEALGNAAQGLPFTAIIDREGRV
                     ARVKLGAFPQDELDRQLAALSGS"
     gene            complement(6691..7308)
                     /locus_tag="PLH85_06915"
     CDS             complement(6691..7308)
                     /locus_tag="PLH85_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64328.1"
                     /translation="MPRPAVPHRKHLRREIAALAARMIAEDGISDYGFAKRKAARQLG
                     AFAADELPNNAEIEAEVRTYLAVFQDEEHLERQLAMRRAALDVMRELEPFRPYLTGAV
                     LEGTAGRYAEVEIDLFPESAKDLDIFLLNQGVPYDHREPRRNQPDGPEAILHLDWDDI
                     PVTLRVYPQDVERLGRRGHGTRPTERARLATVEALVNQTEQTSTP"
     gene            7393..8739
                     /gene="mpl"
                     /locus_tag="PLH85_06920"
     CDS             7393..8739
                     /gene="mpl"
                     /locus_tag="PLH85_06920"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNB64329.1"
                     /translation="MHIHILGICGTFMGGVALLARAAGHTVTGCDANVYPPMSTQLEE
                     QGIGLTEGYGVEQLDLKPDVFVVGNAISRGNPLLEAILDAGLPYVSGPQWLAEHVLQG
                     RWVLAVAGTHGKTTTTSLLAWILQDAGLNPGFLVGGVPKNFGVSARLTESPFFVIEAD
                     EYDTAFCDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRIMPRAGRIV
                     ANAAEETLQRVIGRGCWSELEWFNDAAGWSVAEGAVEDEVVVRHLGREIGRRRLPLSG
                     RHNRSNALAALAAARHVGVTPEIALDALTRFEGVKRRLELRGTVRGVAVYDDFAHHPT
                     AIALTVGGLRRQVGAARILAVLEPRSNTMKLGTMKDQLPVSLTDADKVFCYAAHLGWD
                     AAAALAPIADKSLVTDDFDALIAAIAAEARDGDRILVMSNGGFGGIHGKLLDALAR"
     gene            complement(8757..9800)
                     /locus_tag="PLH85_06925"
     CDS             complement(8757..9800)
                     /locus_tag="PLH85_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNB64330.1"
                     /translation="MSRINRLVLECALALALVVVSLGAYVRLSDAGLGCPDWPGCYGQ
                     LVVPEQAPDLAAARNAFPDRPVEAPKAWKEMIHRYAASGLGLAILALVAMAWRRRAER
                     TPGLEIALLAVVCAQGLLGMLTVTRLLKPVIVTGHLLGGLSTLALLAVLWLRERPAPS
                     RRPAPAWLRSLALAALAAVFVQIALGGWVSSNYAAAACPDFPTCQGRWWPELDTHHAF
                     TLDRELGATASGALLPGTALTAIHWAHRLFAGVVLGLVGTLGVALMGRVGWRGEGLAV
                     LVALSLQLALGIGNVLLHWPLPLAVAHNTGAALLLCATLAATLRLFTQGAVQTARPAR
                     TTGLFAPPPQPLR"
     gene            complement(9797..10699)
                     /gene="cyoE"
                     /locus_tag="PLH85_06930"
     CDS             complement(9797..10699)
                     /gene="cyoE"
                     /locus_tag="PLH85_06930"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNB64331.1"
                     /translation="MPALLHAPTALRAPLTLRLAAFVTQCKPRVNSLIVFTALIGMFL
                     AVPGWPPLGRLLAATLGIALVAGAAAAINCLVERTIDARMARTRARPLPLGLITPAET
                     LGLACLTGGAGLWVLHMAVNPLTMWLTLATFLGYAIVYTLLLKPATPMNIVIGGASGA
                     MPPVLGWAAMTGTIGLPALALFLIIFLWTPPHFWALACYRREEYARAGLPMLPVSHGV
                     PLTCLHILLYTVLLAAVSYLPCTLGLSGGTYALLVSGLNLGYLHQAWRLWRDYSDDRA
                     RGAFRYSLIYLTGLFAALFLDRLL"
     gene            complement(10719..11276)
                     /locus_tag="PLH85_06935"
     CDS             complement(10719..11276)
                     /locus_tag="PLH85_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64332.1"
                     /translation="MTARYRPLWLILGLFLLPLAIGGGLFAYGWRPAAPAANGDLLNP
                     ARTLPELLGADGRPLDPATFTDHWTLLVASAGPCEDACRQWIVATRQVHVALYKQMNR
                     VQRVWLTDQPHPDATPLLTLQPDLHTAIAASEAARQRFDLDRAGPQVFVVDPQGRLIL
                     RYAADADPRGLLKDMERLLRYAWAG"
     gene            complement(11273..12046)
                     /locus_tag="PLH85_06940"
     CDS             complement(11273..12046)
                     /locus_tag="PLH85_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNB64333.1"
                     /translation="MKTTARSSNGVRRLCAGLLAATSGRRFRPSLAGGVVALAVATLC
                     VQLGHWQAGKADQKQAAQARLDRLGHDAAVDITAAPVDAEVLHGRPVRLRGRFETEYQ
                     FFVDNRVHREQAGYHVITPLRLEGSDRRILVNRGWIPALARHSDVPQVDTPTGPVTLD
                     GVAMVPGHRFFTLGTEAASAGWPAVWQNLDLERFARLAGVPVQPVIVQLDPSAPAGFA
                     REWPRADERYERHLSYAYQWYGFGAAAGLIWFALAWRRR"
     gene            12036..12230
                     /locus_tag="PLH85_06945"
     CDS             12036..12230
                     /locus_tag="PLH85_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HNB64334.1"
                     /translation="MVFKLAVVLMLVAVIVSLFSGLFFLYRDKGDGARVVRALTIRIS
                     LSVLLFLALLVAYRFGLYSQ"
     gene            complement(12218..13084)
                     /locus_tag="PLH85_06950"
     CDS             complement(12218..13084)
                     /locus_tag="PLH85_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNB64335.1"
                     /translation="MNASHASTPHTGHYYVPQPSVYPFILSGGMFLLALGFILLMNRF
                     APGPWVMGGGAMVIIGVLFRWFGAVIAENQGGAYTDWEDKSFRQGMVWFIGSEVMFFA
                     AFFGALFYCRTISVPALAGMDPMFTLYEGYTGGWPTAGPKGASFTPMGAWGIPAINTA
                     LLLTSGMTLTWAHWGLMKGKRAQLNLGLILTVLLGVIFLGFQAYEYHHAYTEMGLTMG
                     AGVYGATFFMLTGFHGFHVTLGAIMLAVVSGRCLAGHFDAQRHFAFEAVAWYWHFVDV
                     VWLLLFVVVYWL"
     gene            complement(13103..13666)
                     /locus_tag="PLH85_06955"
     CDS             complement(13103..13666)
                     /locus_tag="PLH85_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNB64336.1"
                     /translation="MTAMHDASLPTSPRHGRLVGRLVLLAGGCFIFGFALVPLYDTLC
                     KAVGFNGKTLKDALSAQEAGATAVDSSRSLNVQFTATVMPGLPWDIRPLEPAFDLHPG
                     ELRTTRFLVRNLSDQPIVGQAVPSVSPTVAASHFRKLDCFCFKQQTLAPGESREMALT
                     FIVDRDIGPDLRELTLAYAFFPAPTPR"
     gene            complement(13805..15409)
                     /gene="ctaD"
                     /locus_tag="PLH85_06960"
     CDS             complement(13805..15409)
                     /gene="ctaD"
                     /locus_tag="PLH85_06960"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNB64337.1"
                     /translation="MNTATHALDAHGDHAHHPTGLLRWLTTTNHKDIGTMYMTFALIM
                     FFVGGAMILGVRAELFQPGLQLMKPEFFNQLIGVHALVMIFAALMPAATGFANWMIPL
                     MIGAPDMALPRLNNWGFWLLPPAAMLLTMPFVLALFGIGDGAVATGWTLYAPLSVQGG
                     IGVDFAIFSIHILGISSIMGSINIIATILNMRAPGMTLMKMPLFCWGWLVTAVLLIIT
                     LPVLAGGVTMLLTDRHFGTHFFDAAGGGDPILFQHLFWFFGHPEVYILLLPVWGLMPH
                     VLSTFSRKPVYGHTAQVWSFIAIGVLGLIVWAHHFLTAGLPVPALLYFMYSTMSISLP
                     LAVLFFCWLATMWRGAMSFETPMLFAVGFIVLFGIAGLTGLVLADVAADAQYHHSYFV
                     VAHFHYALFAGGIMGVMTGVYFWLPKWTGHMYSERLGKLHFWLTVVSFNLTFMPQFFL
                     GLAGMPRRIPDYALQFAEFNAISTVGAFCLGLSQLIFAYNVWRCVKGGEPASDQVWEG
                     TDGLEWELSSPPPYHSWETQPVIESR"
     gene            complement(15420..16598)
                     /gene="coxB"
                     /locus_tag="PLH85_06965"
     CDS             complement(15420..16598)
                     /gene="coxB"
                     /locus_tag="PLH85_06965"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNB64338.1"
                     /translation="MIPTRLPTLRRPFFAAGIGLALSAPAFADYTWNFPTPVTPIARD
                     TLSMHNQFMVIITALFVVVFAIMIYSMVKHRKSTGAQPAGFSGPSGALQWFWVLVPFA
                     MLLFIDFVLMGIPAFHAVIDMEDTRTKADMVLKVTGMQWKWQYEYPDTGVKFVSTLST
                     PREQIEGKEAKGEHYLLEVDNEVVLPVGKKVRVLLTSTDVIHTWWVPQFGVKRDAIPG
                     FLRETWVRIETPGIYRGQCAELCGKDHGFMPVVVRAVPEAEYVAWLDEKKATQAAASA
                     GSDKTWSKDELMAKGKEAYDKTCAACHQPDGKGVPPAFPALAGSKVVNSPLLSPEGKL
                     IPDSHLDRVLNGKAGTAMQAFKNTLSDTDIAAIVTYERNSFGNAMGDMVQPAQIKALR
                     "
     gene            complement(16633..17109)
                     /locus_tag="PLH85_06970"
     CDS             complement(16633..17109)
                     /locus_tag="PLH85_06970"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HNB64339.1"
                     /translation="MALTFQEAGGAREWTARPNRSLSSAARRGLIGGLVIVTLLVSAG
                     FMLAGAWMVLPFAGLELIVLALALRALERSDTAYETIRLESGRLSVLTHCPHGDLSAS
                     FHPFWARVSLEPDGPGGDPLLYIRSHGRHARVGHLMTPDQRRTLAAELRGQLQRLP"
     gene            17230..17853
                     /locus_tag="PLH85_06975"
     CDS             17230..17853
                     /locus_tag="PLH85_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB64340.1"
                     /translation="MTNHTFAMLRCAISGVLAAMLLAGCTPSGEGGGKTQGFVATDIT
                     GADYARGFRLTDHTGRPRTLEDFRGKVVTIFFGYTQCPDVCPTNLLAMAEVMRQLGDD
                     AARVQVLFITVDPERDTPALLAQYVPSFDGRFLGLYGDPAATAAVAKEFRVFYQKQGD
                     VKGGSYTVDHSTGTYVFDPSGRLRLYVKHGEQPDRIAADLRRLLAGQ"
     gene            complement(17979..18833)
                     /gene="rpoH"
                     /locus_tag="PLH85_06980"
     CDS             complement(17979..18833)
                     /gene="rpoH"
                     /locus_tag="PLH85_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNB64341.1"
                     /translation="MTTTLSFPVPSAVGSLDQYIQSVNRIPLLTEKEESDLAHSFRDD
                     GDLDAARKLVMSHLRLVVAIARGYLGYGLPHADLIQEGNVGLMKAVKRFDPTRGVRLV
                     SFAIHWIKAEIHEYIVRNWRLVKIATTKAQRKLFFNLRGMKHDSATLQGDEVLAVAKQ
                     LGVKPEEVREMETRLSGHDVALDAGPDDDEERFAPIAYLADPHAEPSEVLAEQQLARL
                     QDRGLHDALASLDERSRAIVSRRWLSEGDAATLHELAAEYGVSAERIRQIEAKAMQKM
                     RTQLAAFA"
     gene            complement(19018..19806)
                     /locus_tag="PLH85_06985"
     CDS             complement(19018..19806)
                     /locus_tag="PLH85_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HNB64342.1"
                     /translation="MCIATSVGLGGRNDLADVAAIQILLNLNATRFKPPNPPPLGVDG
                     RIGKNTIQRISDFEVQAMGLPQSDGLIAPGDATVRALLAGLPEGPTKEKLSIVLPLSP
                     AERIDHYFDPLKSGMTKYGITTPLQAAHFLAQVAHESASFLYTEEIASGDAYEGRKDL
                     GNTQTGDGRRYKGRGLIQLTGRANYTAYSKACGVDYVAHPEKIADDPLAAVDVACWFW
                     VTRGLPALAEQDDVKAVTKRINGGYNGLDDRIHYLTRAKAVLGL"
     gene            complement(19962..21020)
                     /locus_tag="PLH85_06990"
     CDS             complement(19962..21020)
                     /locus_tag="PLH85_06990"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNB64343.1"
                     /translation="MTFDVRGYAAHSPSDDLVPYRFARRSPRPDDVTIEILFCGVCHS
                     DLHTARNDWRNTVYPVVPGHEIVGRVTSVGPAVTRFRPGDRVGVGCLVDACRHCEACA
                     AGLEQFCQEGPTFTYNSTDRLDRTPTQGGYSERIVTSERFVLRIPDGLDLAGAAPLLC
                     AGITTWSPLRHWQAGPGRRVGVVGLGGLGHMALKLGKALGAELTLFTRSPGKEADARR
                     LGADHIVLSTDAAQMKAVRNRFDLIIDTVPYAHDLNPYLPTLRHDGTLVLVGFLGKLD
                     AMLNTTSLVVGRKAVAGSFIGGLPETQEMLDFCGAHGVTSDVEIIRMQDINAAYARML
                     RSDVKYRFVIDMASLKAV"
     gene            21202..22326
                     /locus_tag="PLH85_06995"
     CDS             21202..22326
                     /locus_tag="PLH85_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64344.1"
                     /translation="MNVRKHLAPALLTVLVGAIGGGIWYSNGQLGEEHAQRQLASQAQ
                     ARHVTLRGLIGSEKEAFFNDPRVRQALEAQGIGVTVEKAGSRTIVAQFDAARYDFGLP
                     SGAPDAERLRQVSKAKRVFNPFYTPMVIASWRPIADILVANGIVERQGELYYLTDLPR
                     LLDLIQHGTRWRELKDSGRFSTSKSVLINSTDIRSSNSAAMYLALASYFGNGQQVVQS
                     QADVERVLPVVAPLFLRQGFQEQSSSAPFEDYLALGMGKTPLLFAYESQLVEFWLRHP
                     DRRKGDMVMIYPRPTVYSKHVLVPYTPAGERLGVVLESDPALRALAHEYGFRTGGDVH
                     GPEEWARQGITVPDTLDDVIDPPSQEWQERLIQAIETRFK"
     gene            22340..23590
                     /locus_tag="PLH85_07000"
     CDS             22340..23590
                     /locus_tag="PLH85_07000"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNB64345.1"
                     /translation="MTTQNPAVAPAASLDLPPPDAFASLTLEAPAPVAPVPVASAGGK
                     VRLKAEDVHALDQMAGQFIDEITRYDSHDPRFKSAVERIHAMGNRDVEASASVSNRML
                     NRPVQTLKNGMFDKSSLIGANLIDLRRTIEDLDPSRQHDLFKPRKILGLIPFGNTLID
                     YFDRYSSSQSHINAIIEGLKRGRDELLRDNAAIEQEKANLWGLMERLEKYIYIGRKLD
                     GELDAKAAQLDLADPDKARVVREEMLFYARQKVTDLLTQMSVNIQGYLALDMIRKNNL
                     ELIKGVDRATTTTVAALRTAVMVAQALANQKLVLDQITALNTTTGNLIAATSVLLKEQ
                     SQAIHEQAASGTIEIAKLQQAFQNIYQTMDAIDDFKLKALASMQTTVETLGSEVEKSR
                     SYLDRVRRREAGNTFEASSAGVSL"
     gene            23587..25155
                     /locus_tag="PLH85_07005"
     CDS             23587..25155
                     /locus_tag="PLH85_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNB64346.1"
                     /translation="MTDRRRGWAAVGAVLLALGLTACGRESGGGAAGTAQGPVTVLAG
                     SELRDVEPQLLKAAADAGVPVKLSYAGTLDIVERVNAGEAVDMILPPSGAYPSLALAT
                     PPRAREKLFYSRVVLGVRSDKARALGWDRRAPAWTDIARAAGAGRLHFAMTNPTSSNT
                     GMSALFAVASAVAGKTEDLSEKEVNGTVLKAFLAGQTLTAGSSGWLAEAYARDPSALD
                     AMINYEAVILRTNASLADKDRLVIIHPRDGVITADYPLMLLNERRRDAYTRLVAAFKS
                     VAVQQNLAASAFLRPALPDVPLAAGVSPEPVVELTFPNRLEVIDAVLQAAQAQWRRPA
                     TSIFVLDVSDSMAGARLDAMRQALLTLVEAKGLAASQRYAGFQPRERVIMIPFSDKVF
                     PPTQVDFGQETLDDGRRQLRTFVNALHIAGGTAIYAALIRADELADAELRQSPDRTVS
                     IVLLTDGINNSGPGYYEFMRHRGADAVRVFPILFGEASVDQMESLASATRGRTFDGRH
                     TALASIFKDIRAYQ"
     gene            25152..25799
                     /locus_tag="PLH85_07010"
     CDS             25152..25799
                     /locus_tag="PLH85_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64347.1"
                     /translation="MNDRSRRPASLRALLFLYSNANIGGCLSALLGPALLFGGVIGPG
                     WMWITAGLYGVGALGGTLLRRTPEVERQIEATLTLDEILQHLDRVIAEVTPEVGRDIL
                     THLASLRSAIAEVLPRLLELSGDHGDLYTVRETVLRYLPETLANYVALPPAFRSAHVL
                     RDGKTARQLLTEQIALLDARMQDIVANVASADAQALLANGEFLRTKFDSADFLPR"
     gene            complement(25820..26488)
                     /locus_tag="PLH85_07015"
     CDS             complement(25820..26488)
                     /locus_tag="PLH85_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64348.1"
                     /translation="MSPNDDELSALLNTHATRHRAGPGLQARVRSRILLAGLRGAAGS
                     ALSRAAGPDVLRWRGMAAGFILGVLATLVGVPWVQQIEQDRSAEAAVVASHVRSLGSG
                     PLIEVASADRHTVKPWFQGRIDYAPPVADLADVGFPLLGGRVDTLAGTRVAGLAYTHR
                     KHVINVFVWPDERVAVLRQAQIRGFNTLQWSNGRMGFRVISDLDAGELGRFGETLRAR
                     TAAP"
     gene            complement(26491..27021)
                     /locus_tag="PLH85_07020"
     CDS             complement(26491..27021)
                     /locus_tag="PLH85_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB64349.1"
                     /translation="MSAARRFESLVLPHLDAAYNLARWLLTDRAAAEDAVQEAALRAF
                     RYFESARGDAIRPWFLGIVRNTCFSHLERTRNRQELGGFDEAALDAAHAAAGHSAPEP
                     GRVVEGRQARARIDAALRALPPPLREVIVLRELEGLEYSEIARISAIPVGTVMSRLSR
                     ARERLRAILGALESES"
     gene            complement(27028..27405)
                     /locus_tag="PLH85_07025"
     CDS             complement(27028..27405)
                     /locus_tag="PLH85_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNB64350.1"
                     /translation="MKTWTRRRLLGGTLSLGAATLLEGLAGRNAVADPPPVIDVEARR
                     FRFTPAEIPLKAGQAVELAIRSVDFVHGFSLPDLGLRTDLPPGRVTRVALPPLPPGTY
                     DFLCDNFCGDGHEEMHGRLIVRA"
     gene            complement(27402..28289)
                     /locus_tag="PLH85_07030"
     CDS             complement(27402..28289)
                     /locus_tag="PLH85_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB64351.1"
                     /translation="MDRRHFLGLSALGGGVVFASGLAGCAGLATAPGATRDFYFVQMS
                     DVHWGYQGEANPDAAITLRKTVQAVNALAVPPAFIVFTGDLTHTTDDPAERRRRMREF
                     KAIVADLQVRTHYYLPGEHDASLDRGAAYREHFGDPNYTFVHEGVHFIALDNVSDPGA
                     RVGEDQLRWLEATLAPLAADTPIVVLTHRPLFDLAPRWDWATRDGAQVMAILQRWRNV
                     TVFYGHIHQEHHRMTGHIPHHSAKSLIFPLPAPGAQEKRTPLPWDAAEPYRGLGFREV
                     HTAAGGSAFGLQEFPVQRS"
     gene            complement(28359..28757)
                     /locus_tag="PLH85_07035"
     CDS             complement(28359..28757)
                     /locus_tag="PLH85_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB64352.1"
                     /translation="MHRLLSLALFGLALCAAMNAHADDAPERGRALYREHCSACHAPD
                     FNGAGPAHRGVVGRLAGTVPGFAYSPALKDAGFVWTPERLDRWLTDPEAVRPGQRMGV
                     SVADAAERADLIAYLRTLGEAPQKETAPRE"
     gene            complement(29390..29815)
                     /locus_tag="PLH85_07040"
     CDS             complement(29390..29815)
                     /locus_tag="PLH85_07040"
                     /inference="COORDINATES: protein motif:HMM:NF017954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF995 domain-containing protein"
                     /protein_id="HNB64353.1"
                     /translation="MDARPIPIACALHLLVAGCVLATPLAWAEGEGVRLSRDELQALI
                     PGSETHHTSSAGSVRSWKNAADGSFVASSDNRKYGSAAGTSNATAPGTWNISDDGKYC
                     VQIEWRRVSEKWCSAIVKGDDGAYYLNSVDPAKRIEFVR"
     gene            30086..30505
                     /locus_tag="PLH85_07045"
     CDS             30086..30505
                     /locus_tag="PLH85_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB64354.1"
                     /translation="MTNRSLQYIVKDQEPLTRGPEASLQEACQDMWTRRVGAVMVVDD
                     GRLVGIFTGRDAVHALSRGFDPRHTPLATAMTPAPDTIPPEATAIDALRRMRDCGYRH
                     LPIVDRGRIVGIVSRGDFQGLELDRLEGEDALWERIC"
     gene            30618..33404
                     /locus_tag="PLH85_07050"
     CDS             30618..33404
                     /locus_tag="PLH85_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNB64355.1"
                     /translation="MAVLFSLLILGVGTAISLYHFGETRHLLDAAQAALFRAVVAEVG
                     RTVDDADRAVRRSFVLLAASSLADATRFGERVRDLPTLTGLLEADPVVDSVMIGYGGG
                     DFLLVRHGRGEVAWEVVDVSRPAGVDAVPVVMVSRYDAHLRLLDARAEDDVSARYEPR
                     LAEWYRLAAAQDALVVTAPGMLPFASRPGVTYARRNGAEVFAVNVGLDNLAGLLARAP
                     VPAGSRLQIADGAGRVVASGGRPDGAGADVMAATLRMSAGSTARRSVHDETGAGWQVS
                     AAPLRGFGEQHWTLAVATPDASLFAEAERQRGHAIRVTVLAMLFSVPLAWGLSRLLTT
                     PLHDLADVARGLSALQFQARPDTRSVILEVDQLARAIGVMRGAIARFLEMGRDLGAAR
                     DVGAVLDDVVNAARDVAGARLCRVEVADDGEGCAEVSRRATGQPLPAGPALQVLSALV
                     YEAEGPVSLADRRTSGPWQYLGIPLRTPDGEALGALLLADRQGPRSGLARAEVAGFLL
                     ALAGSAAVALENQRLLRGRKALLRGVISMVAEAIDAKSPYTGGHCRRVTALAQGLARA
                     AEASSQPSLAQFRLDANGHEALEIACWLHDCGKLTTPEYVIDKGSKLETLYNRIHEIR
                     TRFEVLKRDVEVAYWRGIASGGDAYALRRRRDAAWQSLDDDFAFVARCNRGAEGVSEA
                     DLTRIDRIAARRWVRTLDDRLGLSPEELARHHAPPPPLPVAEALLHDGPEQVIARTDS
                     ALFARGNPWGFTMTVPSRLYDRGEVHNLKVGRGTLTEEERFKIREHIVQTILMLSRLP
                     FPRELAAVPEMAGGHHETPDGRGYPRGLRGDQMSVAARILAIADIYEALTAPDRPYRS
                     ANTPAEALEIMTRLADEQAIDEALFRVFVEAQVWQVDDRPVAPASGLSAIGQQAGQHD
                     KQDECDPQCPADPS"
BASE COUNT         5273 a        10669 c        12566 g         5369 t
ORIGIN      
        1 tcagcgtgtc acgcgggtgg tgcggccgtc ctgcagcagg cgcacgcgct caccgggctt
       61 gaagccctcg ccctcgtctt cctggacgat ggcaagcatt tcaccgttgt ccatcttcag
      121 ggtgatttcc agaccgttct tgcgggtcag gccttcttcg gctgccgagc cggccagacc
      181 gcccaccacg gcaccgacca cggcaccgat gctggagccc ttgcctccgc cgacggtgct
      241 cccggcgatg ccgccaacgg cggcgccgcc cagagtgccg atcggcgact tggtgccttc
      301 gagtttgacg acgcggacgc tttcgatgac gcccatgcgg acattcatga cgcggcgggc
      361 ttcggcacgc tcataggtgc cgccgcccag tccggaggca cagccggtga cggatacgac
      421 ggccacgagg gcgagggaga gggggagcag acgcatggtt tttcctttca ccacagggtt
      481 gtgccgaagg cttcgcgaaa gcgttcggcg atttcgtcgc ggcggggggt gtggttctgg
      541 cccccgcggc tggcaatctt gagggccccc atcagcgacg caaggcggcc ggtgagcgcc
      601 cagtccaggc ccgcctcgat gccgtagagc aggccggcac ggtaggcgtc gccgcagccg
      661 gtggggtctt cgacggccgc cggccggacg cagggaatgt cgatgcgttg cccgccggtg
      721 tggatctcgg agccttcgcc gccgcgtgtg acgatcagtg ccttgagttc tccggacagg
      781 ctggccggcg tccggccggt gcgctcgcac atcaggcgcg cctcgtagtc gttgaccgtg
      841 cagtagtcgg ccagttgcag acaggcgacg agctcttcgc cggagaacat gggcaggccc
      901 tgaccgggat cgaagatgaa cgggatgccg gcttcggcaa actggcggca gtgttcgagc
      961 atgccatcgc ggccgtccgg ggcgacgatg cccaggcgca cgtcgcgggc atcgctcacc
     1021 ttgttgaggt gggactgggt catggcgccc ggatggaagg cagtgatctg gttgtcatcc
     1081 agatcggtgg tgatgaaggc ctgcgcggta taggtgccgg gcacgtgggt gacgtgggca
     1141 cgggacaggc cgagaccgtc gagacgctcc aggtacgggc cggcgtcatc gccgacggtg
     1201 gccatgatca gcggatcgcc gcccaggagc ttgaggttgt aggcgatgtt gcccgcgcag
     1261 ccaccgaact cccgccgcat ctccggcacc aggaaggaga cgttgaggat gtggatctgc
     1321 tcggggagga tgtggttgcg gaagcgatca tggaacacca tgatcgaatc gaaggcgatg
     1381 gagccgcaga tgagggtgga catgggcgtc ttgaagatgt cgttcggatt atagaagccg
     1441 cgcatggatg ccgggatggc atgtgttgcg ggatgtttcg gcgcgctcag gggtggaagg
     1501 cgaggacctg atagcctgct gcggccacgc cgggggcgtc aaagacgatg cgggcggaga
     1561 tttcctggtt gggcgcgaag gccgccttgc gggcatcggg gggcagccat tgggccggtt
     1621 cgagcacgcg gcggacgagg gccttgtcgc gggcgtccgt gagggtgatc tcgaggtagg
     1681 ggtaggcctg ctcgaaatct gcccggttgc gcaaggtggc gtagagggtg aaccgcgctt
     1741 cccggctgcc gtccggctgc atgtcggaac tttcgatggt gatcaggctg gaatcccgcg
     1801 gcaggggcat gtcgcagccg atccgatcgc acagcgcttc gagggccggg cgcagggcgg
     1861 gtgcggcgag ggcgatccgg ttgcggaata cgagcacccc ctgcgcgagc agcgcgacga
     1921 tcaggaaggt gacggcggcg gcccagacgg cctggcgcag accgcggggt ggggcgtcct
     1981 cgtcatccgc tgcggaggtg tcggggccgg cctgcggagc aggcaggacg aagggagcgt
     2041 cgtctgcgtc ggccaccagc gtgaacggct tgggcgcggg gtctggtgct gcgccaggtt
     2101 cgggttcgag gcgcacgtcg ttcggagggg cgccaaagtc gggcggcagc gggaaggccg
     2161 cggcgggcgg cggctctgtg cttgcgccat cgtcgcgcgc tgactcgatc ggggcgggct
     2221 cggtcgcgcg ggcaatctct gcgacgtgct catccacgtc caacgggaga aggtgctcgt
     2281 cgggcggtcc gtcaccgtgt cgcgccggtg ggtcgtcaag gatgaaggtg ggctcgacag
     2341 ggggcgcgtc gtccaggcgc tcgggcaggg tgtgcgtgct tccgtcggga aacggatctg
     2401 caagcggtgc tgcgtcgaag gccggcgtct gggatgtggg ctcgtcagca gcgtcaagca
     2461 gctcgggctc ggcaggtgcg tcgtccggca ggggcggaat ctcgccgatg ggctcgatat
     2521 ggggcgacgg gctgtcaggg tccggggctg tccagtcgtg ccgagccctg acggatttct
     2581 cctcgaggat gaagaccgga ctttcaaccg cttcggcgag gggttcgcca tcgacgctgg
     2641 cgggcggcgg cgggaccggg ttctcgacga gggtttcgag ggcgttgaag gcgtgctggc
     2701 agtggccgca gcgcacgcgg ccctggcggg cgcgcagttg ttccggacga acccggaagg
     2761 tcgtggcgca cgcagggcag cgcgcgagga tcatggcccg gtctgctccg ggcggacgcc
     2821 ttcgagccgc acccagccgt ccgacaggtc cgccacgcgt aatgcgatgt agggcgcgta
     2881 ggctgcgatg acctggtcgg cctgggcttc cagcacgccg gacagggcga tccggccgcc
     2941 aggggcgatg tggctggcca gaagcggtgc cagcatcttg aggggattgg tgaggatgtt
     3001 ggcgaccacg cggtcgaact ggccgtccag agcggtggac gaatgcagga gggtgaggtg
     3061 gacgccgttg ttgcgggcgt tgtcggcggc ggtctcgaca gccttctcgt cgatgtccac
     3121 accggttacc gtgccggcgc cgagacgtgc agcggcgatg ccgaggatgc ctgagccgca
     3181 gccgtagtcg aggacgctct ggccgggctg cagactcgtc gccagccatt ccaggcacag
     3241 gcgcgtggtc ggatgggagc cggtgccgaa tgccatgccg ggatcgagga cgaggttgat
     3301 ggcgtcggga tcgggcgcgg cgtgccacga ggggacgatc cacagtcggt cggtgacgcg
     3361 gatcgggtcg aactggctct gggtgagctg gacccagttc tgctcctcga cttcctcgat
     3421 gtggaagggg ggcacggcgt cgaggccggc ctcaccggct gcggccgaca gggcgctggc
     3481 caggtcggcg tccgggtcga agagggcgac gacgcggctg tggtcccaca ggctggccgg
     3541 atgcatgccg ggctcgccga actggggtcg ctcggcctcg gtgccggcgt cggcgtcgtc
     3601 gatgctcacc gacagggcgc cgtgctccat gagggcttca gagagggtct cggcgcgggc
     3661 cgcgtcggcc tgcaggatga cggagagcca catggtgtca gccctgtttg acctcgttgc
     3721 gggcggccag cttgtgctcc aggtagtgga tgctggtgcc accctcgatg aacttggcgt
     3781 cacgcatcag gtcctggtgc agggcgacgt tggtcttgat gccttccacc atcatctcgg
     3841 agagggcgat gcgcatgcgc cggatggcct ggtcgcgggt gtcgccgtag gcgatcagct
     3901 tgccgatcat ggaatcgtag tgctggggta ccgtgtagcc gttgtacacg tgcgaatcca
     3961 cgcggatgcc aggaccgccg ggcacgtgcc agtgggtgat gcggccgggg cagggggtga
     4021 acttaaaggg gtcctcggcg ttgatgcggc actcgatggc gtggccgcgc aggacgatgt
     4081 ccttctggcg gtagcgcagc ttctggccgg cggcgacgag gatctgttcc tggacgatgt
     4141 ccacgccggt gatgagttcg gtgaccgggt gttccacctg gacccgggtg ttcatctcga
     4201 tgaagtagaa ctcgccgttc tcgtagagga attcgaaggt gccggcaccc cggtagccga
     4261 tcttgcggca ggcctcggca cagcgttcgc cgatgcgggt gatgtgacgg cgctcgatgc
     4321 ccggcgcggg ggcttcctcg atgaccttct ggtggcgacg ctgcatggag cagtcccgct
     4381 cgcccaggta gatggcgttg ccgtgctggt cggcgaggac ctggatttcc acgtggcgag
     4441 ggttttccag gaatttttcc atgtacacca cgggattgcc gaaggccgcc tgggcttcgg
     4501 cgcgggtggt ggtgacggcg ttgaggaggg cggcttcggt gtgcaccacg cgcatgccgc
     4561 gcccgccgcc gccgccggcg gccttgatga tcaccgggta gccgatgctg cgggcgatct
     4621 tgacgatctc cttgggatcc tccgggaggg cgccttccga gccgggcacg cagggtacgc
     4681 cggccgcctt catggcgtcc ttggcggaaa ccttgtcacc catcaggcgg atggtttcgg
     4741 cgcgggggcc gatgaagacg aagccggact gctcgacccg ctcggcgaag tcggcatttt
     4801 cggagaggaa gccgtagccg gggtggatgg cttccgcgtc ggtgacctcg gcggcagaga
     4861 tcagggccgg gatgttgaga tagctgaggg cggacgaggc cgggccgatg cacacggatt
     4921 cgtcggccag ccgcacgtac ttggcatctg catccggctc ggagtgcacg gcgacggtct
     4981 tgatgcccag ctcgcggcag gcgcgcagca cccgcagggc aatctcgccg cggttggcga
     5041 tgaggatctt ttcgaacatg gccatggcga tcagccgatg atgaagaggg gttcgccgta
     5101 ttcgacgggt tcgccgttct cgacaaggat ggccttcacg acgccggcca catccgcctc
     5161 gatctcgttc atcagcttca tcgcctcgat gatgcacagg gcttcgccgg ccgcaatgct
     5221 ctggccgact tcgacgatgg gcttggcgcc cggcgcgctg gcccggtaga aggtgccgac
     5281 catcggggac ttgaccacgt ggccatcggg cagggccggt tctgcggcgg gcgctgccgc
     5341 ggcgggggcc gctgctggcg caggcacatt gatggccatc ggggccggcg ccgcgtaggc
     5401 cactggcgcc gggccggcgc tgtgtttggc gatgcggacc ttttcctcgc cttcggtgac
     5461 ttcgagttcg gaaatgcccg attcctggac caggtcgatc aatttcttga gcttgcgaag
     5521 atccatagtg ttccccttga atgatgacaa cccgccgtcc ggcgggccgt gatgtcgatt
     5581 tcaggcgcct ggcgcggtgc gcaggcgggc gaggacgaat tccagggcga gctggtagcc
     5641 ctgggcgccc agtccgcaaa tgacgcccac cgccttgtcc gagaaatagg agtgatggcg
     5701 gaagggctcg cgggcgtgga tgttggacag gtgcacctcg acgaagggaa tcgcgacccc
     5761 cgtgagggca tcccgcaggg cgacactggt gtgcgtgtag cccgccgggt tgatgatgat
     5821 gaacgcgacg ccttcgcttt cggcagcctg gacccggtcg atcagttcgc cttcgtggtt
     5881 gctctggaag gattccagac acacgccgtc ggcgcgggca cggctttcca tggcgccatg
     5941 gatgtccgcc agggtggtga gaccatagat gccgggctca cgccggccca gcaggttgag
     6001 attggggccg tggaggacaa ggattcgggt ttgcggaagc tcgccgacgg tgccttcctg
     6061 agtgcgattt tttcgcgtca tggcggcaag tttgccgcaa ttcggcgcac gttgtccacc
     6121 tcggcagcgc cagcccggtg cgtcagctgc cgctcagggc ggccagttgc cggtccagtt
     6181 cgtcctgcgg gaatgcgccc agcttgacgc gggcgacgcg accttcccgg tcgatgatgg
     6241 ccgtgaaggg cagtccctgg gccgcgttgc cgagggcttc ggtgagggtg atgacgtccg
     6301 gcgaggcgat caggagcggg taggggacgg gtgatttggc agcgaattcg gcgacgttct
     6361 tgggactgtc gatgctgatg cccacgaact ggaccttgcg ccccttgtac ttgtcgctga
     6421 gggtcgcaaa ttccggcatc tccttgcggc agggggggca ccaggtggcc cagaagttca
     6481 ccaccagcac ctgaccacgc cattgttgca gggcctgttg cgtgccggcg ctgtcgggca
     6541 gcgagagcgc catcaggcgt gctgtggcat cgcgggcagc ggcggtcggc gctggtgacg
     6601 gcgggtcggt gacgtgctgg cgggtgagga ggccggcggc ggctgcaagc aggccgaggg
     6661 cggcaattgc gctccaggtg gtcaggcgag tcatggcgtc gaggtctgtt ccgtttgatt
     6721 gaccagggct tcgacggtgg cgagccgggc gcgttccgtc gggcgcgtgc catggccgcg
     6781 gcgtccgagg cgttccacgt cctggggata gacccgcagg gtgaccggaa tgtcgtccca
     6841 gtcgagatgc aggatggcct cggggccgtc cggctggttg cggcggggct cgcggtggtc
     6901 gtaggggacg ccctggttga ggaggaagat gtccaggtcc ttggcgcttt ccgggaagag
     6961 gtcgatttcc acctcggcat agcggcctgc ggtgccttcg aggacggcgc cggtgagata
     7021 ggggcggaag ggctccagtt cgcgcatcac gtcgagggcg gcgcggcgca tggcgagctg
     7081 gcgttccagg tgttcctcgt cctggaagac ggcgaggtag gtgcggacct cggcttcgat
     7141 ttcggcgttg ttggggagtt cgtcggcggc gaaggcgcca agctggcggg ccgccttgcg
     7201 tttggcgaaa ccgtagtcgc tgatgccgtc ttcggcaatc atgcgggcgg cgagggctgc
     7261 gatctcgcgg cgcaggtgct tgcggtgggg aacggcggga cgcggcatgg gcagacggtg
     7321 cactccgttc gattacaatg ccggccattc tacacacgac tgttgcggcg ctgtttccgc
     7381 gacgaagcga ccatgcacat tcacattctg ggcatctgcg ggaccttcat ggggggcgtt
     7441 gccctgctgg cccgggcggc cggtcatacc gtgacggggt gcgatgccaa cgtctatccg
     7501 cccatgagta cccaactgga ggagcagggc atcgggctga ccgaaggcta tggcgtcgag
     7561 cagctcgacc tgaagcccga cgtcttcgtg gtgggcaatg cgatctcgcg cggtaacccg
     7621 ctgctggagg cgatcctcga tgccggtctg ccctacgtgt cggggcccca gtggctggcg
     7681 gagcacgtcc ttcaggggcg ctgggtgctg gcggtggcgg gcacccacgg caagaccacg
     7741 accacgtccc tgctggcgtg gattctccag gacgcggggc tgaatcccgg cttcctggtg
     7801 ggcggtgtgc cgaagaactt tggcgtgtcg gcacgcttga ccgaatcgcc cttcttcgtc
     7861 atcgaggccg atgagtacga cacggcgttc tgcgacaagc gctccaagtt cgttcattac
     7921 cgcccgcgga cggcgatcct gaacaatctg gagttcgatc atgcagacat ctttgccgat
     7981 ctggcggcga tcgagacgca gttccaccat ctggtgcgga tcatgccgcg tgccgggcgt
     8041 atcgtcgcca acgctgccga agagaccctg cagcgggtga tcggccgcgg ctgctggtcc
     8101 gaactggaat ggttcaacga tgcggcgggc tggtcggtgg cggaaggggc ggtggaggat
     8161 gaggtggtgg tgcgtcatct gggccgggag atcggtcgcc ggcgcctgcc cctgtccggt
     8221 cggcacaacc gttcgaatgc cctggcggcc ctcgccgccg cgcgtcatgt gggcgtgacg
     8281 ccggaaatcg ccctggacgc actgacgcgc ttcgagggcg tcaagcggcg tctggaatta
     8341 cgcggcactg tgcgcggggt ggcggtgtac gacgatttcg cccatcaccc gaccgccatc
     8401 gccctgacag tgggcggcct gcgccgccag gtcggggcgg cgcgcatcct ggcggtgctg
     8461 gagccgcgct ccaacacgat gaagctgggc accatgaagg atcagctgcc ggtcagcctg
     8521 accgacgcgg acaaggtctt ctgctacgcc gcccacctgg gctgggatgc cgcggcggcg
     8581 ctggcgccga ttgccgacaa gtccctcgtg acggacgatt tcgacgcgct gatcgcggcc
     8641 atcgccgccg aggcccggga cggcgaccgc atcctggtga tgagcaacgg cggcttcggc
     8701 ggcatccacg gcaagctgct cgacgccctc gcccgctgag gggccgtccg ccccggtcag
     8761 cgcagcggtt ggggcggcgg tgcgaagagg cctgtggtgc gggcagggcg ggccgtctgc
     8821 actgcgccct gcgtgaacag gcgcagcgtc gccgccagcg tggcgcacag gagcagggcc
     8881 gcgcctgtgt tgtgggcgac ggccaagggc agaggccaat gcagcaggac gttgccgatg
     8941 cccagcgcga gctgcaagct gagggccacc agcacggcaa gcccttcgcc ccgccagccg
     9001 acgcgaccca tcagggcgac gcccagggtc ccgacgaggc ccagcacgac cccggcgaag
     9061 agccggtgag cccagtggat tgcagtcagg gcggtaccgg gcagcagggc gccgcttgcc
     9121 gttgccccca gttcccggtc cagggtaaag gcgtggtggg tgtccagttc gggccaccag
     9181 cggccctggc aggtggggaa atccgggcag gccgccgccg catagttgct gctcacccag
     9241 ccacccaggg cgatctggac gaagactgcc gccagcgcag caagcgcgag gctgcgcagc
     9301 cacgcggggg cgggacggcg ggacggggcg gggcgttccc gcagccacag caccgccagc
     9361 agcgccaggg tgctcaggcc acccagcagg tggccggtga cgatcaccgg cttgaggagt
     9421 cgcgtgaccg tgagcatgcc cagcaggccc tgggcgcaga cgacggcaag caacgcgatt
     9481 tccagcccgg gcgtccgttc ggcgcgtcgg cgccaggcca tggcgaccag cgcgaggatg
     9541 gccagcccta gaccgcttgc cgcgtagcga tggatcatct ccttccaggc cttgggggct
     9601 tccacgggtc tgtcgggaaa ggcgttgcgg gcggcggcca gatcgggggc ctgttccggc
     9661 acgacgagct ggccgtagca gccgggccag tccgggcagc ccagaccggc atccgacagg
     9721 cgcacgtaag cgcccaggga gactacgacc agcgccagcg ccagcgcgca ctccagcacg
     9781 aggcggttga tgcggctcac agcaggcggt ccaggaagag ggcggcgaac aggcctgtga
     9841 gatagatcag cgagtagcgg aaggcgccgc gggcgcgatc gtccgagtag tcgcgccaga
     9901 gccgccaggc ctggtgcaga taccccaggt tgaggccgct gacgagcagg gcgtaggtgc
     9961 cgccggacag gcccagggtg cagggcagat aggacacggc ggccagtagt acggtgtaga
    10021 gcaggatgtg caggcaggtg agcggcacgc cgtggctcac cggcaacatg ggcaggccgg
    10081 cgcgggcgta ttcctccctc cggtagcagg ccagggccca gaagtggggc ggtgtccaca
    10141 ggaagatgat gaggaacagg gcgagggccg gcaggccgat ggtgccggtc atcgcagccc
    10201 agccgaggac gggcggcatg gcgccggacg cgccgccgat gacgatgttc atgggcgtcg
    10261 cgggtttgag gagcagggtg tagacgatgg cgtagcccag gaaggtggcg agggtcagcc
    10321 acatggtcag gggattgacc gccatgtgca gtacccacag cccggcgccc ccggtcaggc
    10381 aggcgaggcc gagggtctcc gcaggggtga tcaggccgag gggcagtgga cgggcgcggg
    10441 tgcgggccat gcgtgcgtcg atggtgcgtt ccaccaggca attgatggcc gcggcggccc
    10501 ccgccacgag ggcgatgccc agagtggcag ccagcaggcg gccgaggggc ggccagccgg
    10561 gcacggcaag gaacatgccg atcagtgcgg tgaacacgat caggctgttc acccggggct
    10621 tgcactgggt gacaaaggcg gccaggcgca gcgtcagggg ggcgcggagg gcggtggggg
    10681 cgtgcaggag ggcaggcata gggtctccgg ggtgagcgtc agccggccca cgcgtagcgg
    10741 agcaggcgtt ccatgtcctt cagcaggccg cgggggtctg cgtcggcggc gtagcgcagg
    10801 atgaggcggc cctgcgggtc tacgacaaag acctgcgggc cggcgcgatc caggtcgaag
    10861 cgctggcggg cggcttcgct ggctgcgatg gcagtgtgca ggtcgggctg cagggtgagg
    10921 agcggggtgg cgtccggatg aggctggtct gtgagccaga cacgctggac gcggttcatc
    10981 tgcttgtaga gggctacatg tacctggcgg gtggcgacga tccattgccg gcaggcgtcc
    11041 tcgcagggac cggcggatgc gaccagcaga gtccagtggt ccgtaaaggt tgcagggtcc
    11101 agcgggcgac cgtccgcgcc aaggagttcg ggcagcgtgc gcgcggggtt cagcaggtcg
    11161 ccattggccg ccggggcggc aggtcgccag ccgtaggcga acaggccgcc gccgatggcg
    11221 aggggcagca ggaacaggcc gaggatcagc cacagggggc ggtagcgggc ggtcaccgac
    11281 gcctccatgc gagggcgaac cagatcaggc ccgcggcggc gccaaagccg taccactggt
    11341 aggcgtagga caggtggcgc tcgtaacgct cgtcggcgcg gggccattcg cgggcaaagc
    11401 cggccggcgc cgacgggtcg agctggacga tgacgggctg caccggaacg ccggcgaggc
    11461 gggcgaaacg ctccagatcc aggttctgcc agaccgcggg ccatccggcg ctggcagctt
    11521 cggtgccgag ggtgaagaaa cggtggcccg ggaccatcgc gacgccgtcg agtgtcaccg
    11581 ggccggtggg cgtgtccacc tgggggacat cgctatggcg ggccagggcg gggatccagc
    11641 cccggttcac gaggatgcgc cggtcgctgc cttcgaggcg caggggggtg atcacgtggt
    11701 aacccgcctg ttcccgatgg acgcggttgt ccacgaagaa ctggtactcg gtttcgaagc
    11761 ggccccgcag gcggacggga cggccgtgga ggacctcggc gtccacgggc gcggcggtga
    11821 tatcgacggc ggcgtcgtgg ccgaggcggt cgaggcgggc ctgggcggcc tgtttctgat
    11881 ctgccttgcc ggcctgccag tgaccgagtt gtacacagag cgtcgccacg gccagggcca
    11941 cgacgccgcc cgccagggac ggccggaagc ggcggccgct tgtggcggcc agcaggccgg
    12001 cgcacaatcg ccgcacccca ttcgaggagc gggcagtggt cttcaagttg gcagtggtgc
    12061 tcatgctggt ggcggtgatc gtcagcctgt tttccggcct gttcttcctc tatcgggaca
    12121 aaggggacgg cgcccgcgtg gtgcgtgccc tgacgatccg catctccctg tccgttctgc
    12181 tctttctggc cttgctggtg gcgtaccggt ttgggcttta cagccagtag acgacgacga
    12241 agagcagcag ccagaccaca tccacgaagt gccagtacca cgccaccgcc tcgaaggcga
    12301 agtggcgctg ggcgtcgaaa tgaccggcca gacagcggcc ggaaaccacc gcgagcatga
    12361 tggcgccgag ggtgacgtgg aatccgtgga agcccgtgag catgaagaag gtggcgccat
    12421 atacgccggc tcccatcgtc aggcccatct cggtgtaggc gtggtggtat tcgtaggcct
    12481 ggaagccaag gaagatcacc cccagcagca cggtgaggat caaccccagg ttgagctggg
    12541 cacgcttgcc cttcatcagg ccccagtggg cccaggtgag ggtcatgccc gaggtgagga
    12601 gcagggcggt gttgatggcc gggatgcccc aggcgcccat gggcgtgaag ctggcgccct
    12661 tggggccggc ggtcggccag ccgccggtgt agccctcgta gagcgtgaac atcggatcca
    12721 tgcccgccag cgccggcacg gagatggtgc gacagtagaa cagggcgccg aagaaggcgg
    12781 cgaagaacat cacctcggag ccgatgaacc acaccatgcc ctggcggaag gacttgtctt
    12841 cccagtcggt gtaggcgccg ccctgatttt cggcaatgac ggcaccgaac cagcggaaca
    12901 gcaccccgat gatgaccatc gcgccgccac ccatgaccca cggacccggc gcgaagcggt
    12961 tcatgagcag gatgaatccc agggcgagca ggaacatgcc gcccgacagg atgaagggat
    13021 agacgctggg ctgcgggacg tagtagtgac cggtgtgagg cgtggaggcg tgagaggcgt
    13081 tcaaggcggg ctccctgatt gtttagcggg gcgtgggggc gggaaagaag gcgtaggcca
    13141 gggtcagttc gcggaggtcc gggccgatgt cgcgatccac gatgaaggtc aacgccatct
    13201 cgcggctttc gccgggggcg agggtctgtt gcttgaagca gaagcaatcc agcttgcgga
    13261 agtgggacgc cgccaccgtg ggtgacacgc tgggtaccgc ctggccgacg atgggctgat
    13321 cggacaggtt gcgcaccagg aagcgggtgg tgcgcagttc gcccggatgc aggtcgaagg
    13381 cgggttccag cgggcggatg tcccagggca ggccgggcat caccgtggcg gtgaattgca
    13441 cattgaggct gcggctgcta tccaccgcag tggcgccggc ctcctgggcg gagagggcgt
    13501 ccttgagggt cttgccgttg aaaccgacgg ccttgcacag ggtgtcgtag aggggcacca
    13561 gggcaaagcc gaaaatgaag cagcctcccg ccagcagcac cagacggccg accaggcggc
    13621 cgtggcgggg cgaagtgggc aggctggcat cgtgcatcgc ggtcatcggg atctcagcgc
    13681 tggacgaaga agccgagggt gtagagggcc actgcggcgg cacccgccca ccaggcgagg
    13741 cgggcagagg cgcggcggcg acgggccaga tcggcgtcgg agatcatctg catggggtgg
    13801 ctcctcagcg gctctcgatg acgggctggg tttcccagga gtggtagggc gggggcgagg
    13861 acagttccca ctccaggcca tcggtgcctt cccagacctg gtcgctggcg ggttcgccgc
    13921 ccttcacgca gcgccacacg ttgtaggcga agatcagctg ggacaggccc aggcagaagg
    13981 cgccgacagt gctgatggcg ttgaactcgg caaactgcag ggcgtagtcc gggatgcgcc
    14041 ggggcatgcc ggcaaggccc aggaagaact ggggcatgaa ggtcaggttg aagctgacga
    14101 ccgtgagcca gaagtgcagc ttgccgagcc gttcgctgta catgtgaccg gtccacttgg
    14161 gcagccagaa atacacaccg gtcatcacgc ccatgatgcc gccggcaaag agggcgtagt
    14221 ggaagtgggc gacgacaaag tagctgtggt ggtactgggc atcggcggcc acatcggcca
    14281 gcaccaggcc ggtgaggccg gcgatgccga acagtacgat gaagcccacg gcgaagagca
    14341 tcggcgtctc gaagctcatg gcgccgcgcc acatggtggc gagccagcag aagaacagca
    14401 ccgccagggg cagggagatg gacatggtgc tgtacatgaa atacagcaga gctggcacgg
    14461 gcaggccggc ggtcaggaaa tggtgggccc acacgatgag gcccaacacg ccgatggcga
    14521 tgaaggacca gacctgggcg gtatggccat aaaccggctt gcggctgaag gtggacagca
    14581 cgtggggcat caggccccac accgggagaa gcaggatgta cacctcgggg tggccgaaga
    14641 accagaacag gtgctggaac aggatcgggt cgccgccgcc cgccgcgtcg aaaaagtggg
    14701 tgccgaagtg gcggtcggtg aggagcatgg tgacgccacc ggcgagcacc ggcagggtga
    14761 tgatgagcag caccgcggtg accagccagc cccagcagaa gaggggcatc ttcatcagcg
    14821 tcatccccgg cgcgcgcatg ttgaggatgg tggcgatgat gttgatggag cccatgatgg
    14881 agctgatgcc gaggatgtgg atcgagaaga tcgcgaaatc cacgccgatg ccgccctgca
    14941 ccgagagggg cgcgtagagc gtccagccgg tggccacggc gccgtcgccg atgccgaaga
    15001 gggccagcac gaagggcatc gtgagcagca tggcagccgg cggcagcagc cagaagcccc
    15061 agttgttgag gcgcggcagg gccatgtcgg gggcgccgat catcagcgga atcatccagt
    15121 tggcaaagcc cgtcgcggca ggcatcaggg ccgcgaagat catcaccagt gcatgcacgc
    15181 cgatgagctg gttgaagaac tccggcttca tcagttgcag gccgggctgg aagagttcgg
    15241 cccggacgcc gaggatcatc gccccgccca cgaagaacat gatgagggcg aaggtcatgt
    15301 acatcgtccc gatgtccttg tggttggtgg tggtgagcca ccggaggaga ccggtcgggt
    15361 ggtgggcgtg gtcgccgtgg gcgtcgaggg cgtgggtggc ggtgttcatg gggtgctcct
    15421 cagcgaaggg ccttgatctg tgccggctgg accatgtcac ccatggcgtt gccgaaactg
    15481 ttgcgctcgt aagtgacgat ggcggcgatg tccgtgtcgg acagggtgtt cttaaaggcc
    15541 tgcatggcgg tgccggcctt gccgttgagc acccggtcca ggtggctgtc ggggatcagc
    15601 ttgccctcgg gcgagagcag cgggctgttg accaccttgc tgccggccag cgccgggaag
    15661 gctggcggga cccccttgcc gtcgggctgg tggcaggcag cgcaggtctt gtcgtaggct
    15721 tccttgccct tggccatcag ttcgtccttg ctccaggtct tgtcgctgcc ggcgctggcg
    15781 gcggcctggg tcgccttctt ttcgtccagc caggccacgt attcggcctc gggcacggcg
    15841 cgcaccacca cgggcatgaa gccgtggtcc ttgccgcaca gctcggcgca ttggccgcgg
    15901 tagatgccgg gcgtctcgat gcgcacccag gtctcccgca ggaagccggg gatggcgtca
    15961 cgcttcacgc cgaactgggg tacccaccag gtgtggatca cgtcggtgga ggtgagcagc
    16021 acgcgcacct tcttgcccac cggcaggacg acctcgttgt ccacctccag caggtagtgt
    16081 tcgcccttgg cttccttgcc ctcgatctgc tcccgcgggg tggacagggt gctgacgaac
    16141 ttgacgccgg tgtccgggta ttcgtactgc cacttccatt gcatgccggt gaccttgagg
    16201 accatgtcgg ccttggtcct ggtgtcttcc atgtcgatca ccgcatggaa ggcggggatg
    16261 cccatcagca cgaagtcgat gaagagcagc atggcgaagg gcaccaggac ccagaaccac
    16321 tggagcgctc ccgaaggccc gctgaatccg gcgggctgcg cgccagtgct cttacggtgc
    16381 ttgaccatcg aataaatcat gatggcgaag acgaccacga acagcgccgt gatgatcacc
    16441 atgaactggt tgtgcatcga cagcgtgtcg cgggcgatgg gggtgacggg cgtgggaaag
    16501 ttccacgtgt agtcggcgaa ggcaggggcg gacagcgcca ggccgatgcc ggccgcaaag
    16561 aatggccggc gaagggtggg gagtcgggtg gggatcaagt ctcttcctct tctgggcatg
    16621 gacgggaggg ggtcagggca gccgttgaag ctggccgcgc agttcggcag ccaatgtgcg
    16681 acgctggtcg ggcgtcatca ggtgtcccac gcgggcgtgg cggccatggg agcggatgta
    16741 cagcaggggg tcgccgccgg ggccgtcggg ttcaaggctg acccgcgccc agaaaggatg
    16801 gaagctggcg ctcaggtcgc cgtgcggaca gtgggtcagc acgctgagac ggccggattc
    16861 gaggcggatg gtttcgtagg cagtgtcgct gcgctcaagg gcgcgcaggg ccagggcgag
    16921 gacgatcagt tccagcccgg caaagggcag taccatccag gcgcccgcca gcatgaaccc
    16981 tgcgctgacg agcagggtca caatgacaag tccgccgatc aggccgcggc gggcagcaga
    17041 actcagagag cggttggggc gcgcggtcca ttcccgcgcg ccgcccgctt cttgaaaggt
    17101 cagagccatg gcgtctcctc caggctttgt ctgcgacttt ttgacgcaga tcaaatttgt
    17161 gggcgcagtt tagcatccgc ttacagtttt gcggtgcacc ggattttttg cgtaacctgc
    17221 tgattaaaaa tgactaacca tacttttgcg atgttgcggt gcgcaatttc aggtgttctg
    17281 gcggccatgc tgctggcggg atgcacgccg tccggcgagg ggggcgggaa gacacagggc
    17341 tttgtggcga cggacatcac gggggcggac tatgcccgcg ggttccggct caccgatcac
    17401 acgggccggc cgcgcacgct ggaggacttt cgcggcaagg tggtgacgat cttcttcggc
    17461 tatacccagt gccccgacgt gtgtccgacc aatctgctgg cgatggccga ggtgatgcgc
    17521 cagctcgggg acgacgcggc acgggtccag gtgctgttca tcaccgtcga tccggagcgg
    17581 gatacgcccg ccctgctggc ccagtacgtg ccgtcatttg atgggcgttt cctgggcctg
    17641 tacggcgatc cggcggcgac tgcggccgtg gccaaggaat tccgcgtgtt ctaccagaag
    17701 cagggcgacg tgaagggcgg tagctacacg gtggaccatt ccaccgggac ctacgtgttc
    17761 gatccctccg gccgcctgcg tctgtacgta aaacacggtg agcagccgga ccgcatcgcg
    17821 gccgatctcc ggcgcctgtt ggcagggcag tgagtcggcc ggtcgtcgcg ggagccggcg
    17881 gtgccgggtg ggtgtccgcg cgcccgggtg gggcgcggtg cggcgctgaa tgaaaaaggg
    17941 cagcccgcag gctgccccta atccgacagt ggcgtcgctc aggcgaaggc ggcgagttgc
    18001 gtacgcatct tctgcatggc cttggcttcg atctgacgga tgcgctcggc cgatacgccg
    18061 tattcggcgg ccagttcgtg gagcgtggcg gcgtcgcctt cactcagcca gcggcggctg
    18121 acgatggcac gactgcgctc gtccaggctg gccagggcgt cgtgcaggcc gcggtcctgc
    18181 aggcgggcaa gctgctgctc ggccagcacc tcggacggct cggcgtgcgg gtcggccagg
    18241 taggcgatgg gggcgaagcg ctcctcatcg tcgtccggcc cggcgtccag cgccacgtcg
    18301 tgaccggaca ggcgggtttc catctcccgg acctcctcgg gcttgacgcc gagctgcttc
    18361 gccacggcca gtacttcgtc gccctgcagg gtggcgctgt cgtgcttcat gccgcgcagg
    18421 ttgaagaaca gcttgcgctg ggccttggtg gtggcgatct tgaccagacg ccagttgcgc
    18481 acgatgtatt cgtggatctc ggccttgatc cagtggatcg cgaaggacac cagacgcacg
    18541 ccgcgcgtcg ggtcgaaacg cttgacggcc ttcatgaggc ccacgttgcc ttcctggatc
    18601 aggtcggcgt ggggcaggcc atagcccagg tagccgcggg cgatggccac caccagacgc
    18661 aggtgggaca ttacgagctt gcgggccgcg tccaggtctc cgtcatcccg gaagctgtgg
    18721 gcgagatcgg actcctcctt ctcggtcagc agcgggatgc gattgacgct ctggatgtac
    18781 tgatccaggc tgccgaccgc ggagggaaca ggaaacgaca gggttgtggt catgggtaag
    18841 gctctccttg cgcccaaagt ttagcactcc atgccagaga gtgctaacag cgccaggagt
    18901 tcccgtgccg cgggtcggtg ccgcgggaag tccgccacgc gtgttgccgg ccctcaccgg
    18961 ccggggcccc ggcttccccc gcgggcgggg gaccggggcg cccgaccggg agggtgatca
    19021 caggccgagg acggccttgg cgcgggtcag atagtggatg cggtcgtcca gaccgttgta
    19081 gccgccgttg atgcgcttgg tgaccgcctt cacgtcgtcc tgctcggcca gcgcgggcag
    19141 cccgcgggtg acccagaacc agcaggccac atccacggcg gccagcggat cgtcggcaat
    19201 cttttcaggg tgcgccacgt aatcgacgcc gcaggccttg gagtacgcag tgtagttggc
    19261 ccggccggtc agctggatca gcccgcgccc tttgtagcgc cggccgtcgc ccgtctgggt
    19321 gttgcccagg tccttgcggc cttcataggc atcgccgctg gcgatctctt cggtgtacag
    19381 gaacgaggcg gattcgtggg cgacctgggc caggaagtgg gccgcctgca ggggcgtggt
    19441 gatgccgtac ttggtcatgc cggacttgag cggatcgaag taatggtcga tgcgctcggc
    19501 cggcgagagg ggcagcacga tggacagctt ctccttggtc ggcccttccg gcaggccggc
    19561 cagcagggcg cggacggtgg cgtcgccggg ggcgatcagg ccgtcggact ggggcaggcc
    19621 catggcctgc acttcgaagt cggagatgcg ctggatggtg ttcttgccaa tgcggccgtc
    19681 cacccccagc ggtggggggt tgggcggttt gaagcgggtg gcgttgaggt tgagcaggat
    19741 ctggatggcg gccacgtcgg cgaggtcgtt acggccgccg agtccgacgg aggtggcgat
    19801 gcacatggtg tttctcctgg agggcttgca aaagggggcg cgacctatcc ggtatatatc
    19861 acccacccgc catgtcaaat ccgggtccgc gccgggcgca ctgccgtgaa gggcctgaag
    19921 ccctcgccgc gcagggcgag ccggcgccgg aggcgcccgc gtcagaccgc cttgagcgag
    19981 gccatgtcga tcacgaagcg atatttcacg tcgctgcgca gcatgcgggc gtaggcggcg
    20041 ttgatgtcct gcatgcggat gatctcgaca tccgaggtga ccccgtgggc gccgcagaag
    20101 tcgagcatct cctgggtctc gggcaggccg ccgatgaagg agccggccac cgccttgcgg
    20161 cccaccacca gggacgtggt gttgagcatc gcatccagct tgccgaggaa gcccaccagc
    20221 accagggtgc cgtcgtggcg cagggtgggc aggtaggggt tgaggtcgtg cgcgtagggt
    20281 acggtgtcga tgatcaggtc gaagcggttg cgcacggcct tcatctgcgc ggcatcggtg
    20341 gacagcacga tgtggtcggc gcccaggcgg cgggcgtcgg cttccttgcc gggcgagcgg
    20401 gtgaacaggg tgagttcggc gcccagggcc ttgcccagct tgagggccat gtggccgaga
    20461 ccgcccaggc ccaccacgcc cacgcggcgg cccggtccgg cctgccagtg gcgcaggggc
    20521 gaccaggtgg tgatgccggc gcacagcaag ggcgcggcgc cggccagatc gaggccatcc
    20581 gggatgcgca ggacgaagcg ttcggacgtg acgatccgct ccgaatagcc gccctgggtg
    20641 ggcgtgcggt cgaggcggtc ggtgctgttg taggtgaagg tcgggccttc ctggcagaac
    20701 tgttcgagcc ccgccgcgca ggcctcgcag tggcggcagg cgtctaccag acagcccacg
    20761 ccgacccggt cgccgggccg gaagcgggtc accgccgggc ccacgctggt cacacggccg
    20821 acgatctcgt gaccgggcac caccggatag accgtgttgc gccagtcgtt gcgggcggtg
    20881 tgcaggtccg aatggcacac gccgcagaag aggatctcga tcgtcacgtc gtcgggtcgc
    20941 ggactgcggc gggcaaagcg gtagggcacc aggtcgtcgc tgggagagtg ggcggcatag
    21001 ccgcggacgt cgaaggtcat ggtcgtcgct ccaggagtcg gggtcggccg ccgggccacg
    21061 gccgcagcgg cagtgtacgc ccggcgtaca tctctgggcg gtgccggttc gggcgccggc
    21121 ggcggcgcaa caaaatcgtc atgaaatcta aatatcatgc ctgttgatga aacagttcga
    21181 cactcggacg aggagcgtgg ggtgaacgtc cgcaagcatc tcgcccccgc cctcctgacc
    21241 gtgctggtgg gcgccatcgg cggcggcatc tggtattcca acgggcagct gggcgaggag
    21301 cacgcccagc gtcaactggc cagccaggcg caggcccgcc acgtcaccct gcgcgggctg
    21361 atcggctcgg agaaggaggc cttcttcaac gatccgcgtg tgcgccaggc actggaggcc
    21421 cagggcatcg gcgtgaccgt ggagaaggcg ggttcgcgca ccatcgtggc ccagttcgat
    21481 gccgcccgct acgacttcgg gctgccctcc ggcgcaccgg atgccgagcg cctgcgccag
    21541 gtctccaagg ccaagcgcgt cttcaatccc ttctacacgc cgatggtgat cgccagctgg
    21601 cggccgattg ccgacatcct ggtggccaac ggcatcgtcg agcgccaggg cgagctgtac
    21661 tacctgaccg atctgccccg cctcctcgac ttgatccagc atggcacgcg ctggcgcgag
    21721 ctcaaggaca gcgggcgttt ctccaccagc aagtcggtgc tgatcaactc caccgacatc
    21781 cgcagctcca acagcgcggc catgtacctg gcgctggcca gctatttcgg caacggccag
    21841 caggtggtgc agagccaggc ggacgtggag cgggtgctgc ccgtcgtcgc accgctgttt
    21901 ttgcgccagg gctttcagga gcagtcctcg tcggcgccct tcgaggacta cctcgccctg
    21961 ggcatgggca agacgccgct gctgttcgcc tacgaatccc agctcgtgga gttctggctg
    22021 cgccatccgg accgccgcaa gggcgacatg gtgatgatct acccgcgccc cacggtgtat
    22081 tccaagcacg tgctggtgcc gtacacgccg gccggcgaac ggctcggggt ggtgctcgag
    22141 agcgatcccg ccctgcgcgc gctggcccac gaatacggct tccgcaccgg cggcgacgtg
    22201 catggccccg aggaatgggc cagacagggc atcaccgtgc ccgacaccct ggacgacgtg
    22261 atcgacccgc ccagccagga atggcaggag cgtctgatcc aggccatcga aacccgcttc
    22321 aagtaaggag gcgccttcca tgacaaccca gaaccccgct gttgccccgg ccgcgagcct
    22381 cgacctgccg ccgcccgacg cgtttgcgtc cctgaccctg gaagcgcccg cgccggtggc
    22441 cccggtgccg gtggcgtcgg ccggcggcaa ggtgcgcctg aaggccgaag acgtgcacgc
    22501 cctggaccag atggccggcc agttcatcga cgagatcacc cgctacgaca gccacgatcc
    22561 gcgcttcaag tcggcggtgg agcgtatcca cgccatgggc aaccgggatg tggaagcgtc
    22621 cgcgtcggtg tccaaccgca tgctcaaccg cccggtgcag acgctcaaga atggcatgtt
    22681 cgacaagagc agtctgatcg gtgccaacct catcgacctg cggcgcacca tcgaggatct
    22741 ggacccgtcc cgccagcacg atctcttcaa gccgcgcaag attctcggtc tgatcccctt
    22801 cggcaacacg ctgatcgatt atttcgaccg ctacagctcg tcccagtccc acatcaacgc
    22861 catcatcgaa ggcctcaagc gcggccgcga cgagctgctc cgcgacaacg cggccatcga
    22921 acaggagaaa gccaacctgt ggggcctgat ggagcgcctc gagaagtaca tctacatcgg
    22981 ccggaagctc gacggcgagc tggacgccaa ggccgcccag ctcgacctgg ccgatccgga
    23041 caaggcccgc gtggtgcgcg aggagatgct cttctacgcc cgccagaagg tcaccgacct
    23101 gctcacccag atgagcgtca acatccaggg ctacctggcc ctggacatga tccgcaagaa
    23161 caacctggag ctcatcaagg gcgtggaccg ggccaccacc accaccgtgg cggccctgcg
    23221 caccgcggtg atggtggccc aggccctggc caaccagaag ctcgtgctcg accagatcac
    23281 cgcgctcaac accaccaccg gcaacctcat cgccgccacc agcgtgctcc tcaaggagca
    23341 gagccaggcc atccacgagc aggccgcgtc cggcacgatc gagatcgcca agctgcagca
    23401 ggccttccag aacatctacc agaccatgga cgccatcgac gacttcaagc tcaaggcgct
    23461 ggcgtccatg cagaccacgg tggagaccct cggcagcgag gtggaaaagt cccgcagcta
    23521 tctcgaccgg gtgcgtcggc gcgaggcggg caacaccttt gaggcttcgt cggccggagt
    23581 gagcctgtga cggaccgccg ccgcgggtgg gctgcggtcg gggccgtgct gctggcgctg
    23641 gggctgacgg cctgtgggcg cgagtcgggc ggcggggccg ccgggaccgc ccaggggccg
    23701 gtgaccgtcc tcgccggctc cgagctgcgc gacgtggagc cccagctcct caaggcggca
    23761 gctgatgcag gcgtgccggt gaaactcagc tacgccggca ccctggacat cgtggagcgg
    23821 gtcaatgccg gcgaagcggt ggacatgatc ctgccgccca gcggcgccta cccgtccctc
    23881 gccctggcca cgccgcccag ggcgcgggaa aagctcttct attcccgcgt ggtgctgggc
    23941 gtgcggagcg acaaggccag ggccctgggc tgggaccggc gggcgccggc gtggacggac
    24001 atcgcgcggg cggctggcgc cgggcgactg cactttgcga tgaccaaccc gacgtcgtcg
    24061 aacaccggga tgagcgccct cttcgcggtg gcttccgcgg tggcgggcaa gaccgaggat
    24121 ctgagcgaaa aagaggtgaa tggcaccgtc ctgaaggcct tcctggctgg ccagacgctg
    24181 actgccggca gctccggctg gctcgccgaa gcctatgccc gcgacccctc cgctctggac
    24241 gcgatgatca actacgaagc ggtgatcctg cgcaccaacg ccagtctggc ggacaaggac
    24301 cgcctcgtga tcatccaccc ccgcgacggg gtgatcaccg cggactaccc gctgatgctc
    24361 ctcaacgagc gtcggcgcga cgcgtacaca cgcctcgtgg cggccttcaa gtcggtcgcg
    24421 gtgcagcaga atctggccgc gagcgccttc ctgcggccgg ccctgcccga cgtgcccctc
    24481 gccgccggcg tatcgccgga gcccgtggtg gagctgacct tccccaaccg gctggaggtc
    24541 atcgacgccg tcctgcaggc ggcgcaggcc caatggcggc ggcccgccac ctccatcttc
    24601 gtgctcgacg tcagcgactc catggccggc gcccgcctcg acgccatgcg tcaggcgctg
    24661 ctgacgctcg tggaggcgaa ggggctggcg gccagccagc gctacgccgg ctttcagccc
    24721 cgcgagcggg tgatcatgat ccccttctcg gacaaggtct tcccgccgac gcaggtggac
    24781 ttcggccagg aaaccctcga cgacgggcgt cggcagctgc gcaccttcgt gaacgcgctg
    24841 catatcgctg gcggaacagc catctatgcg gcgctgatcc gcgccgacga gctggccgac
    24901 gcggagctca ggcagtcgcc cgaccggacc gtgagcatcg tgctcctgac cgacgggatc
    24961 aacaattccg ggccgggcta ctacgaattc atgcggcacc gtggcgcgga cgccgtccgg
    25021 gtctttccca tcctgttcgg ggaggccagc gtcgaccaga tggagtccct ggcctcggcc
    25081 acccgcgggc gcacctttga cggacgccac actgccctgg cgagcatctt caaggacatc
    25141 cgcgcctacc aatgaacgac cgctcccgcc gcccggcgtc cctgcgtgcg ctgctcttcc
    25201 tctacagcaa cgccaacatc ggcggctgcc tgagcgcgct gctcgggccg gccctgctct
    25261 tcggcggcgt catcggtccc ggctggatgt ggatcaccgc cggcctgtac ggcgtgggcg
    25321 ccctgggcgg cacgctgctg cggcggacgc ccgaggtgga gcggcagatc gaggcgacgc
    25381 tcaccctcga cgagatcctc cagcacctgg accgcgtcat cgccgaggtg acgcccgagg
    25441 tcgggcggga catcctgacg cacctggcga gcctgcgcag cgccatcgcc gaggtgctgc
    25501 cgcgcttgct ggagctgtcc ggcgaccatg gcgacctcta caccgtgcgc gagacggtgc
    25561 tgcgctacct gccggagacc cttgccaact acgtggccct gccgcccgcc ttccgcagcg
    25621 cccacgtgct gcgggacggc aagaccgcgc gccagctcct caccgagcag atcgccctgc
    25681 tcgacgcacg gatgcaggac atcgtggcca acgtggcgag tgccgatgcc caggccctgc
    25741 tcgccaacgg cgagttcctg cgcaccaagt tcgattccgc cgacttcctg ccgcgctgaa
    25801 cggcgcgtcg ggcgctgctt cagggcgccg cggtgcgggc ccgcagcgtc tcgccgaacc
    25861 ggcccagctc gccggcgtcc agatcggaga tcacccggaa gcccatccgg ccattgctcc
    25921 actgcagcgt gttgaagcca cggatctggg cctggcggag gaccgccacg cgctcgtcgg
    25981 gccacacgaa aacattgatg acgtgcttgc ggtgcgtgta ggcaaggccc gccacgcgcg
    26041 tgccggcgag ggtgtccaca cgcccgccga ggagcggaaa gcccacgtcc gccagatccg
    26101 cgaccggcgg cgcgtaatcg atgcgcccct ggaaccaggg cttgacggtg tgccgatctg
    26161 ccgacgcaac ctcgatcagc ggaccgctgc ccaggctgcg cacatgactg gcgaccacgg
    26221 cggcctcggc ggagcggtcc tgctcgatct gctgcaccca cggcacaccc accagggtcg
    26281 cgagcacgcc gaggatgaag ccggcggcca tcccgcgcca gcgcaggacg tcgggacctg
    26341 ccgcccggga cagggcgctg cctgccgcac cgcgcaggcc cgccagcagg atgcggctgc
    26401 gcacgcgggc ctgcagaccg ggccccgcgc gatggcgggt cgcgtgggtg ttgagcaggg
    26461 cggacagttc gtcgtcgttc ggactcatct tcaggactcg ctttccaggg cgccgagaat
    26521 ggcccgcagg cgctcgcggg cgcgggacag gcgggacatc accgtaccga ccgggatcgc
    26581 ggagatccgg gcgatctccg aatattccag gccttccagt tcgcgcagca cgatgacctc
    26641 gcggagcggc ggcggcaggg cgcgcagggc cgcgtcgatc cgcgcgcgcg cctgccggcc
    26701 ttccacaacc cgccccggct ccggcgcgct gtggccggcc gcggcgtggg ccgcgtccag
    26761 cgctgcctcg tcgaagccgc cgagctcctg gcggttgcgg gtgcgctcca gatgactgaa
    26821 gcaggtgttg cgcacgatgc ccagaaacca gggccgtatc gcgtcgccgc gggcgctctc
    26881 gaagtagcgg aaggcgcgca gggccgcctc ctgcaccgca tcctcggccg ccgcccggtc
    26941 ggtcagcagc cagcgggcca gattgtaggc ggcgtccagg tgcggcagca cgaggctttc
    27001 gaaacggcgg gcggcgctca cgacggatca ggcacggacg atcaggcgtc cgtgcatctc
    27061 ctcgtggccg tcgccgcaga agttgtcgca caggaagtcg taggtgccgg gcggcagggg
    27121 cggcagggcg acccgtgtca cccgccccgg cggcagatcg gtgcgcaggc cgaggtccgg
    27181 caggctgaaa ccgtggacga aatccaccga gcggatcgcc agttccaccg cctggccggc
    27241 cttgagcggg atctcggccg gcgtgaaacg gaagcggcgg gcttccacgt cgatgacggg
    27301 cggcgggtcg gccacggcgt tgcggccggc cagcccttcc agcagggtgg cggcgccgag
    27361 gctgagggtg ccgcccagga ggcggcgtcg ggtccaggtc ttcatgagcg ttgcaccggg
    27421 aattcttgga ggccgaaagc gctgccgccg gcggcagtat ggacttcccg gaagccgagg
    27481 ccgcggtaag gttcggcggc atcccagggt aatggcgtgc gtttctcctg ggcgccgggc
    27541 gccggcagcg gaaagatcag ggattttgcg gaatggtgcg ggatgtggcc ggtcatgcgg
    27601 tgatgctcct ggtggatgtg gccatagaac acggtcacgt tgcgccagcg ttgcaggatc
    27661 gccatgacct gcgcgccgtc ccgcgtggcc caatcccagc ggggcgccag gtcgaacagg
    27721 gggcgatggg tgagcacgac gatgggggtg tccgccgcaa ggggcgcgag ggtcgcctcc
    27781 agccagcgga gctggtcttc accgacgcgg gcgccggggt ccgagacatt gtcgagggcg
    27841 atgaaatgca cgccctcgtg gacgaaggtg taattcgggt cgccgaagtg ttcccggtag
    27901 gcggcacccc ggtcgaggga ggcgtcgtgt tcaccgggga gatagtagtg ggtgcggacc
    27961 tgcaggtccg ccacgatggc cttgaactcg cgcatccggc gccggcgctc ggccgggtcg
    28021 tcggtggtgt gcgtcaggtc gccggtaaag acgatgaagg ccgggggcac tgccagggca
    28081 ttgaccgcct gcaccgtctt gcgcagcgtg atggccgcat cggggttggc ctctccctga
    28141 tagccccagt gtacgtccga catctggacg aagtagaagt cccgcgtggc gccgggcgct
    28201 gtggccagcc cggcgcaacc ggccagtccg gaggcgaata ccacgccgcc gccgagggcg
    28261 ctcaggccca gaaaatgccg tcgatccatg tccttgctcc tgttgggtga cggacgcccg
    28321 ctgcgccctc cctgtgccat accggatggg gcgcgggttt attcccgcgg ggcggtctct
    28381 ttttgcggcg cctcgccgag ggtgcgcaga taggcgatca gatcggcccg ctcggccgca
    28441 tcggcgacgc tcacccccat gcgctgcccc ggacggacgg cctccgggtc ggtcagccag
    28501 cggtcgaggc gctcgggcgt ccagacaaat ccggcgtcct tcagcgcagg ggaataggcg
    28561 aagccgggca cggtgccggc caggcggccg acgacgcccc ggtgggcggg gccggcgccg
    28621 ttgaagtcgg gggcatggca ggcgctgcag tgctcccgat acagggcacg tccccgctcg
    28681 ggcgcgtcgt cggcgtgggc gttcatggcg gcacagaggg caagcccgaa cagggcgagg
    28741 gagaggaggc ggtgcatgac ggaactccag gcggggtgat ggggagacgc cgacggtgat
    28801 gccggtgtcg ccctgtttac cgcccgtgag gcggattcat tcccggcgtg gcccgaaggt
    28861 ggccgacgtg gcttgcctcg agactgcctg caccgcggcg gcccgctgac ggccggcctg
    28921 gtggcgaccc ggagtgcccg cgggctgcgc cccgaatcgg caaggcgcgg cccacccact
    28981 gctgcggtgt catggaccgc cggaatgctg cctcgacgcg ctgagctttt ttggcgatcg
    29041 ctttttcacg ttcgccatcg cacgggtgag cctcgtggat gtggagcgcc aggcggcggt
    29101 ctggcggctc gcgagacgtg ccgggaggtc ggcttctgcg gacatgccaa gcctgccgtt
    29161 ccgcgtacgg ggggtggacg cccggcgcaa tgcacgtttc cgcgacaatc cgcgggcggc
    29221 aggggccccc cttcagctgc gccgatgcgg gcgtaccgct gcgtctgcgg ggcggcgagt
    29281 tcgtcggcac gctgtgcctg atcggcccgc agcccggcac tcaggcggaa cgggccgtct
    29341 ggcggtggga ctggcgcgtg gcgccggccc ctgacgcgcc gcggcggtgt cagcgcacga
    29401 attcgatgcg cttggcgggg tcgacgctgt tcaggtagta ggcgccgtcg tcgcccttga
    29461 cgatggcgct gcaccatttt tccgagacgc gccgccattc gatctgcacg cagtacttgc
    29521 cgtcgtcgga tatgttccag gtgccggggg cggtggcatt ggaggtgccc gcggcgctgc
    29581 cgtacttgcg gttgtccgag gaggcgacga agctgccatc cgccgcattc ttccagctcc
    29641 ggacgctgcc ggcgctggac gtatggtggg tttcgctgcc gggaatcagg gcctgcagtt
    29701 cgtcccggct gagccgaacc ccctcgccct cggcccaggc cagcggggtg gccaggacgc
    29761 agccggcgac gagaaggtga agtgcgcagg caatgggaat ggggcgtgca tccatggtga
    29821 cgatcctcct gacagggtgt ggttcgggcg gtcctcggcc gggtctgcgc tgaagcgtcg
    29881 cgctggcgga cgacgtccag ggggacggga gccctgcgtt tcatgatacg cccgccgccg
    29941 cccggaggcg cccgggtgcg ccggcttgtc acgcggtcgt caaggaactg accctaaact
    30001 gtaccgagcc tgtaacccag gcgggctgcg gcgggactac attcaaagta gtcgatgatt
    30061 tcatcggtcg cgtgcggagc acatcatgac gaaccgcagc ctgcagtaca tcgtgaagga
    30121 tcaggagccc ctgacgcggg ggccggaggc gagcctgcaa gaggcctgcc aggacatgtg
    30181 gacgcgccgc gtcggggccg tgatggttgt ggacgacggc cggctggtgg gtatcttcac
    30241 cggccgggac gccgtccatg ccctgtcccg gggcttcgat ccgcggcata cccccctggc
    30301 gacggcaatg acgccggcac cggacacgat tccgccagag gccaccgcca tcgacgccct
    30361 gcgccgcatg cgtgactgcg gttatcgcca cctgcccatc gtggaccggg ggcggatcgt
    30421 cggcatcgtc tcgcggggcg acttccaggg cctggaactg gaccgcctgg aaggcgagga
    30481 cgcgctctgg gagcgtatct gctgaatggc ccggccggct tccggcgcgc cgggtgggcg
    30541 tgagggtggg ggagggaggc ttcgtgcggg cacgttgggg gacatggccg acgtggcgcc
    30601 tgccgctgca cgttccggtg gccgttctgt tttcgctgct gatcctcggg gtgggcacgg
    30661 cgatctccct gtatcacttc ggtgagacgc gccacctgct ggatgcggcg caggcggccc
    30721 tgttccgcgc cgtcgtggcg gaggtggggc gcacggtgga cgacgcggat cgggcggtgc
    30781 ggcgcagctt cgtgctgctg gcggcttcgt ccctggccga cgccacccgc tttggcgagc
    30841 gggtgcgcga tctgccgacg ctgacgggcc tgctggaggc cgatcctgtc gtcgactcgg
    30901 tgatgatcgg ctacggcggg ggcgacttcc tgctggtgcg gcacgggcgc ggggaggtgg
    30961 cctgggaggt ggtggacgtg agtcgtcccg ccggtgtgga cgccgtgccg gtggtcatgg
    31021 tgtcccgcta tgacgcgcat ctgcgcctcc tcgatgcgcg ggccgaggac gatgtgtccg
    31081 cccgctacga gccgcgtctc gccgagtggt accggctggc cgcagcgcag gacgccctgg
    31141 tggtgacggc gcccgggatg ctgcccttcg cgtcccgtcc gggtgtcacc tacgcccggc
    31201 gtaacggggc cgaggtgttc gcggtgaacg tggggctgga caatctggcc gggctcctcg
    31261 cccgcgcgcc tgtgccggcg ggcagccgtc tccagatcgc cgacggcgcc gggcgcgtcg
    31321 tggcgagcgg cggccgtccg gacggggcgg gcgcggatgt gatggccgcc accttgcgga
    31381 tgtccgccgg ctcgaccgcc cgccggtcgg tgcacgatga gacgggcgcc ggctggcagg
    31441 tgtccgccgc accgctgcgg ggctttggcg agcagcactg gacactggcg gtggctacgc
    31501 ccgacgcgag tcttttcgcc gaagccgaac gccagcgagg tcacgctatc cgcgttacgg
    31561 tgctggcgat gctgttcagc gtgccactcg cctgggggct gtcgcgcctg ctcacgacgc
    31621 ccctgcacga tctggcggac gtggcgcgcg gcctgtcggc gctccagttc caggcgcggc
    31681 cggatacccg ctcggtgatc ctggaggtgg accagctggc ccgcgccatc ggtgtgatgc
    31741 gcggtgccat cgcccgcttt ctggagatgg ggcgcgatct gggcgcggcg cgggatgtgg
    31801 gcgcggtcct cgatgacgtg gtgaacgcag cgcgggatgt ggcgggggca cgcctgtgcc
    31861 gggtcgaggt ggccgacgac ggggaaggtt gcgcggaggt gtcgcgccgg gcgaccggtc
    31921 agcccttgcc ggcgggcccg gcgctgcagg tgctgagcgc gctggtgtat gaggccgagg
    31981 ggcctgtgag tcttgccgac cgcaggacct ccggcccctg gcagtacctg ggcattcccc
    32041 tgcgcacacc cgatggcgag gcgctggggg cgttgttgct ggcggaccgg caggggccgc
    32101 gcagcggtct ggcgcgggcc gaggtggcgg gcttcctgct ggccctcgcc ggcagtgccg
    32161 cggtggccct ggaaaaccag cgcctgttgc gggggcgcaa ggcgctgctg cgcggcgtga
    32221 tcagcatggt ggccgaggcc atcgatgcga agtccccgta cacgggcggc cattgccgcc
    32281 gggtgacggc cctggcccag ggcctggcgc gggccgccga ggcgtcttcg cagccgtccc
    32341 ttgcccagtt ccgcctcgac gccaacgggc acgaagccct cgagatcgcc tgctggctgc
    32401 atgactgcgg caagctcacc acgcccgaat acgtcatcga caagggcagc aagctggaga
    32461 ccctgtacaa ccgcatccac gagatccgca cccgcttcga ggtgctcaag cgcgatgtgg
    32521 aggtggcgta ctggcgcggc atcgccagcg gtggtgacgc gtacgccctg cgtcggagac
    32581 gggacgccgc gtggcagtcc ctggacgatg atttcgcttt tgtcgcacgg tgcaaccggg
    32641 gcgccgaagg ggtgtccgag gccgacctga cccgcatcga ccgcatcgct gcccggcgct
    32701 gggtgcggac gctcgacgac cggctgggcc tgtctcccga ggaactggcg cgccaccacg
    32761 cgccgccgcc gcccctgccg gtcgcagaag cgctgctcca cgacgggccc gaacaggtga
    32821 tcgcccgtac cgacagcgcc ctgtttgccc gcggcaatcc gtggggcttc acgatgacgg
    32881 tgccgtcgcg cctgtacgac cggggcgagg tgcacaacct gaaggtcggc cgcggcacac
    32941 tgactgagga ggagcgtttc aagatccgcg aacacatcgt tcagacgatc ctgatgctgt
    33001 cgcgtctgcc cttcccgcgg gagctggccg cggtgccgga gatggccggc ggtcaccatg
    33061 aaacgccgga cggacggggc tacccgcggg gtctgcgggg tgaccagatg agcgtcgccg
    33121 cgcgcatcct ggccatcgcc gacatctacg aggccctcac cgccccggat cgcccctatc
    33181 gcagcgccaa tacgccggcg gaggccctgg agatcatgac gcgcctggcc gacgagcagg
    33241 ccatcgacga ggcgctgttc cgcgtgttcg tggaggcgca ggtgtggcag gtggacgacc
    33301 gcccggtcgc acctgcctca ggcctgtcag cgatcggcca gcaggccggt cagcacgaca
    33361 agcaggatga atgcgatccc caatgcccag cagaccctag ttgagggctt ccaggtgcgc
    33421 cgccggcccg tcccgcggcg gcccacgcca ggacggcggg tccacccgtg cgcggctctc
    33481 gacaatccgt tctccatctg actcatcaca gtgccccgat cctgagtggt tgtaccgcga
    33541 gcgtagcgtc ctgagctgaa cgcagccttc acaaaggctt gcatggggct gaatgagggg
    33601 aggcaggggc gttgacgcgt cccctgcgtg gcagtgccaa gtcccggttg gctgttgccc
    33661 ccggcctatc ggggcgtggg gacggaggcg gattcgcctg gtatgcaggc gtcgggacgt
    33721 ggccgggccc tccgtttcga ccggcagaag ccgcgggtct gcgcgcttga cggaggcacc
    33781 aaaagcaaaa aggccaaccc cggaggattg gcctaagtgc ttgaattctg gtgggttgtg
    33841 agtggctcga acactcgacc tacggattaa gagtccg
//
LOCUS       DAOQMM010000029        33176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_354_length_33176_cov_53.237825, whole genome shotgun sequence.
ACCESSION   DAOQMM010000029 DAOQMM010000000
VERSION     DAOQMM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 33176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33176
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_354_length_33176_cov_53.237825"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLH85_07055"
     CDS             complement(<1..1122)
                     /locus_tag="PLH85_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB64356.1"
                     /translation="MSELRKTLNLPDTPFPMRGDLAKREPGWIADWQQRKLYQKIRKA
                     AAGRPKFVLHDGPPYANGSIHIGHALNKILKDIIVRSKTLAGFDAPYVPGWDCHGLPI
                     EHKIEVTHGKNLPADKVRALCRTYAAEQIEEQKKDFIRLGVLGDWDNPYRTMDFGNEA
                     GEIRALAEMVGRGWVFKGLKPVNWCFDCGSALAEAEVEYQDKQSPAVDVGFPCAEPDR
                     LAAAFGLPALPADKTAWAVIWTTTPWTIPANQALNVHPEHAYALVDTPRGLLVLMAEL
                     ADASLKRFGLEGRVIARTDGRSLDRIAFRHPFYDRLSPVFVADYVGLDAGTGIVHSAP
                     AYGLEDFNSCKANGFSNDDILTPVQSNGVYADTLPFFGGL"
     gene            complement(1136..2068)
                     /locus_tag="PLH85_07060"
     CDS             complement(1136..2068)
                     /locus_tag="PLH85_07060"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNB64357.1"
                     /translation="MQVLRGIPDHAEHGCVLTIGNFDGVHRGHQALLARLAAKARETG
                     LPAVVLTFEPHPREYFTHEVRPVRLTSLREKVGLLEQHGVDRIVIGRFNERFAGLSAE
                     AFIEDVLIHGLGVRHLLIGDDFRFGRGRKGDFALLRATGERAGFTVEAMPTLADADGR
                     VSSSAVREALAAGDMPRAARLLGRPYSISGRVLHGDKIGRTIGFPTANIQLKHRSPPL
                     MGIFTVSVDGVAERPWPGVASIGVRPTINDAGRPSLEVHLFDWHGDCYDAHLRVNFLA
                     WQRAEERYDSLDALTAQIARDADQARAHFARNPL"
     gene            complement(2140..3252)
                     /gene="tgt"
                     /locus_tag="PLH85_07065"
     CDS             complement(2140..3252)
                     /gene="tgt"
                     /locus_tag="PLH85_07065"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNB64358.1"
                     /translation="MKYELLATDGAARRGRLTLNHGTVDTPAFMPVGTYGSVKGISPQ
                     DLRDIGAQICLGNTFHLWLRPGLDVMTAFGGLHQFMAWDGPLLTDSGGFQVFSLGALR
                     KISEEGVRFASPVNGDKLFLTPEESMRIQRVLNSDVAMIFDECTPYPASETEAGDSMR
                     LSLRWARRSRDEFDRLENPNALFGIVQGGMYESLRDESLAGLTEIGFHGYAIGGLSVG
                     EPKEDMQRILHHTAPRLPKDSPRYLMGVGTPEDIVFAVQAGIDMFDCVMPTRNARNGW
                     LFTRHGDIKIRNARHKADTRPLDDSCDCYTCRHFSRGYLHHLNRLNEILGARLATIHN
                     LHYYQQLMRELRDAIAHGRLTDYVARFHRERAGQDA"
     gene            complement(3249..4277)
                     /gene="queA"
                     /locus_tag="PLH85_07070"
     CDS             complement(3249..4277)
                     /gene="queA"
                     /locus_tag="PLH85_07070"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNB64359.1"
                     /translation="MALTLDDFDYPLPPELIAQAPLPRRTDSRLLHVAEPLADLHFAD
                     LPALLRPDDLLVFNDTRVLHARLFGVKDSGGRVEVMIERPLDEHEALAQIRASKSPKA
                     GTRLRLEDALDVDVLGRAGDFFHLRFPPHAPLVTLLERHGRLPLPPYIERAAGEADES
                     RYQTVYARHPGSVAAPTAGLHFDDALLAQLAAQGVRSATVTLNVGAGTYQPVRVQDLT
                     QHRMHTEAYHVPDATVAAIDTARATGGRVICVGTTSLRALESAARDGSLRAGQAESDL
                     FILPGYQFRVADALITNFHLPKSTLLMLVSAFAGMDTLRSAYAHAVAQRYRFFSYGDA
                     MFLTRKPA"
     gene            4321..4405
                     /locus_tag="PLH85_07075"
     tRNA            4321..4405
                     /locus_tag="PLH85_07075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4355..4357,aa:Leu,seq:caa)
     gene            4653..5606
                     /locus_tag="PLH85_07080"
     CDS             4653..5606
                     /locus_tag="PLH85_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB64360.1"
                     /translation="MALDPQARALLDMVYRVGAPRFHELDVHQARHSFEKLLFALRPE
                     APPVASVTEVPIPRAHARDGVIMARLYRPLNSHADEALPITFYFHGGGWCVGNVASYD
                     VLGRQLANLSGGAVLSVDYALAPEHPFPGAVVDAQRAVDWALANAVLLGVDPARFALA
                     GDSAGGTLSAVLAHQMRNARVHAARLQLLIYPCVEILSQRPSRKTYSNGYFLDNESMA
                     WFFDKYLPNAEDWSDWRASPLLAPRFDDLPPAAIITAGCDPLTDDCLAYADALRAAGV
                     PVSVHGFDGMVHGFFTLGKFFPQANEAVTRAGEALRDALKA"
     gene            complement(5629..6522)
                     /locus_tag="PLH85_07085"
     CDS             complement(5629..6522)
                     /locus_tag="PLH85_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB64361.1"
                     /translation="MPEAVPPSSEYAVLFADLVGSTELYERVGDKAAFEQVDRCIRGM
                     RIAVETKQGRVVKHTGDGLMAVFRDADSAADATLLIHQTVKELPAAGDQKLALRIGFH
                     FGPVVVAGTDVFGETVNFAARLAELASPGKAITSSETARRLGPEWRSVLHTLPPRAIR
                     GLSRPVEVCELMCEAVGELTIVQSDHFLLATEPELRLYLDSKTLLLNATRPSARIGRD
                     TAADLVVADTQSSRRHAEIELRGDKFVLIDRSSNGTFVQIDGEKEFLLSREEVVLRGR
                     GHFSLGRSCANSPQVVDFVCL"
     gene            6776..8020
                     /locus_tag="PLH85_07090"
     CDS             6776..8020
                     /locus_tag="PLH85_07090"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB64362.1"
                     /translation="MKRVDDFRLRFGRQEYVPIMIGGMGVDISTADLALEAARLGGVG
                     HISDAMVKTVADRRFKTKYVKEKLQQYKYNVANPDKSEVQFDLGHLAEATHLHVGRTM
                     EAKRGQGLIFVNCMEKLTMNAPKETLKVRLTAAMDAGIDGITLAAGLHLGSFALIEDH
                     PRFRDVKLGIIVSSLRALQLFLRKSARTQRLPDYVVVEGPLAGGHLGFGMDWAQYDLA
                     TIVAEIRDWLKVEQLDIPLIPAGGIFTGSDAAAFLEAGAGAVQVATRFTVSQECGLPD
                     AVKQQYFRADEDDIEVNQISPTGYPMRMLKNSPAIGSGIRPNCEAYGYLLDAKGSCAY
                     IEAYNRELALHPAEKKLSVLDKTCLCTHMRNFDCWTCGHFTYRLKDTTRLDENGQYRL
                     LTAEHIFRDYQFSTDQKIALPD"
     gene            complement(8108..9097)
                     /locus_tag="PLH85_07095"
     CDS             complement(8108..9097)
                     /locus_tag="PLH85_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNB64363.1"
                     /translation="MTADHHPDSVLFQGEKPFPVLPAVDHYAGSEKLMRKALALQQEI
                     GPLFDITCDCEDGAHAGAEAEHARMAASIVMSGDNRFGRVGARIHDITHPHWEQDVDI
                     LVGEAGARLAFLTLPKPRSVADAARQIDALRSAEERHGIEQAIPVHVLIETHGALRDA
                     WEIAALDRVESLDFGLMDFVSGHHGAIPGAAMRSPGQFEHPLIIRAKCDIAAAALAHG
                     VVPSHNVTTELKDLAYIRRDAERARREFGYLRMWSIHPNQILPIVEAMRPDFSEVEEA
                     TAILAGAQDAGWGPIQHAGKLHDRASYRYYWELLKRARATGMNLPDDALARFF"
     gene            complement(9195..10187)
                     /locus_tag="PLH85_07100"
     CDS             complement(9195..10187)
                     /locus_tag="PLH85_07100"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNB64364.1"
                     /translation="MAKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDL
                     PQAQKAVKGVIMELEDCAFPLLAGVIATDDPNVAFKDAQVALLVGARPRGPGMERKDL
                     LTENAKIFTVQGAAIGQYADPDVKVLVVGNPCNTNAYIAKEIAVKYGRVQAKNFTGML
                     RLDHNRALSQLATKTGRDVSSLKNLVVWGNHSPTMYADYRFATSNGDKVPALVNDEAW
                     NRDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGSNGEWVTMGIPSDGSY
                     GIPEGIIYGFPVTTVNGEYTIVQGLEIDEFSRSKMTNTLNELLEERDGVKDLLG"
     gene            10418..11146
                     /locus_tag="PLH85_07105"
     CDS             10418..11146
                     /locus_tag="PLH85_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB64365.1"
                     /translation="MRQDSPTFSPLYRQIKNLMMNALESAEWRPGQAIPSEQELALRF
                     SVSQGTVRKAIDEMAAENLLVRKQGKGTYVASHNDPRALFRFLRLVPIDGELAHPQSI
                     PLDCWRAKAGNEAARMLGIELGAPVTILRRLLKFGGRPVVVDEIYLPGEVFHGLTMEV
                     LQGSTGSLYGLFEARFGIRMIRAEERIRAVAADRMVGETLGVAEGTPLLSVERVTYTY
                     GDRPVEWRRGLYSTADHYYLNELS"
     gene            11262..11651
                     /gene="sdhC"
                     /locus_tag="PLH85_07110"
     CDS             11262..11651
                     /gene="sdhC"
                     /locus_tag="PLH85_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNB64366.1"
                     /translation="MTEVTLKKQRPKHLALNEIRLPLPGKVSILHRVSGAGLFLMLPV
                     LLALFGASVGSPESYAAYKDVVSNVFVKLLLVGLLWAFLHHFCAGIRFLLLDVHVGVD
                     KESAVSSARTVLIVSLALTVILVAKLW"
     gene            11645..11992
                     /gene="sdhD"
                     /locus_tag="PLH85_07115"
     CDS             11645..11992
                     /gene="sdhD"
                     /locus_tag="PLH85_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNB64367.1"
                     /translation="MVKPIIVGAHYGFRDWLAQRITAVVMAVYSILFAVSALATLDGT
                     YESWRGLFSGGFMKFATFLFFMSLFYHAWIGVRDIFMDYIKPTGLRLTLHSVVALLLI
                     GYAGWAAQILWRL"
     gene            11993..13786
                     /gene="sdhA"
                     /locus_tag="PLH85_07120"
     CDS             11993..13786
                     /gene="sdhA"
                     /locus_tag="PLH85_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNB64368.1"
                     /translation="MKVAKRTFDAVIVGAGGAGLRAALQLSEAGMKTAVLTKVFPTRS
                     HTVAAQGGVAASLGNSTEDNWHWHMYDTVKGSDWLGDQDAIEFMVKKANEVVVELEHY
                     GMPFDRTDNGKIYQRPFGGHSQNYGQTPVSRSCAAADRTGHAMLHALYQRNVRANTQF
                     FVEWMALDLLRDAEGNVLGVTAMEMETGEVSIFHSRATIFATGGAGRIYYSSTNAFIN
                     TGDGVGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILRNASGERFMERY
                     APNLKDLASRDVVSRSMVTEINEGRGCGSDKDHVLLDITHLSPETIMKRLPGIREISI
                     QFAGVDPIKAPIPVVPTCHYQMGGIPTNYKGQVVVPQDGNPNVPVHGFYAAGECACAS
                     VHGANRLGTNSLLDLLVFGKSAGETVVADFKAGQLPLKPLPDNAADVSLARIARLEGQ
                     TGGEEVADVRIAMQRTMQKHAGVFRFADMLKEGAAKILEVAERAKVTQIKDKSQVWNT
                     ARIEALELDNLIEVAKATMVSAEARKESRGAHVRDDAIDCAETPNGRDDKNWLKHTLW
                     YSEGNRLDYKPVNLQPMSPEVEPIALAKRTY"
     gene            13807..14517
                     /locus_tag="PLH85_07125"
     CDS             13807..14517
                     /locus_tag="PLH85_07125"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNB64369.1"
                     /translation="MNKRTVQFKIYRYDPDKDNQPYMQDISVELEESDKKLLDALVRL
                     KAKDDSLSFRRSCREGVCGSDAMNINGKNGLACLTGIDDLAQPITIRPLPGLPVIRDV
                     IVDMTQFFKQYHSIKPYLINNEPAPERERLQTPEDREELNGLYECILCACCSTSCPSF
                     WWNPDKFVGPAGLLAAYRFLADTRDQATSERLDNLEDPYRLFRCHSIMNCVDVCPKGL
                     NPTKAIGKIKDMMLRRAV"
     gene            14514..14750
                     /locus_tag="PLH85_07130"
     CDS             14514..14750
                     /locus_tag="PLH85_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNB64370.1"
                     /translation="MTLNRGRVRWQCRRALLELDLVFTRFLDTHFDHLTDDQVADLES
                     LLVVEDHDLWAMVNGSKPVSDDRWKEMVKLLRAG"
     gene            14808..16103
                     /gene="gltA"
                     /locus_tag="PLH85_07135"
     CDS             14808..16103
                     /gene="gltA"
                     /locus_tag="PLH85_07135"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNB64371.1"
                     /translation="MTTQRTAALNVDGKSVEFPIMSGTHGNDVIDIRTLGGKTGLFTY
                     DSGFLSTASCQSKITYIDGDKGELLYRGYPIEQLAEQCNFLEVTYLLKNGELPNTTQK
                     VDFENTIKRHTMVHEQLAKFYSGFRRDAHPMAVMTGVVGALSAFYHDAMDFADAEHRN
                     VSFNRLIAKMPTIVAMAYKYNMGMPFMYPDNELDYTANFMRMMFGNPCEKYVPNPTLV
                     RALDIIFTLHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEACLKM
                     LEEIGDVSRVGEYINRAKDKNDTFKLMGFGHRVYKNFDPRAKLMRKVCYDVLTELGLE
                     NDRLFKLAMELEKIALEDQYFVEKKLYPNVDFYSGIVQKALGIPTEMFTCIFALARTV
                     GWMTQWEEMITDPEYKIGRPRQLYIGAARRDVVPLAQRG"
     gene            16266..19091
                     /locus_tag="PLH85_07140"
     CDS             16266..19091
                     /locus_tag="PLH85_07140"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNB64372.1"
                     /translation="MMKQLFNTSQLFGSNAPFIEELYEAYLDNPDSVSDEWRSYFDTL
                     QNTPGNGRDVAHYPIINAFADMAKRGPVRTVAAGGGDQKQVSVLQLINAHRFLGTRWA
                     QLDPLKRTERPEIHELELSHYGFTEGDLNQTYNTGSFHGLPGDHATLREILDAVRQTY
                     CGSIGAEYMYITDIAEKRWIQARLEPTRGKPAFPAEQQRRILERLTAAETMERYLHTK
                     YVGQKRFSLEGGESTIVAMDELIRVAGSQGVAEIVIGMAHRGRLNVLVNTLGKSPKML
                     FSEFEGKAASDLSAGDVKYHMGFSSDVMTSGGPVHLTLAFNPSHLEIVNPVVEGSVYA
                     RQTRRGENGKAEVIPVIIHGDAAVAGQGVNQEMLNFAQTRGYGTGGTIHVVVNNQIGF
                     TTSDPRDYRSSLYCTDIFKMAEAPIFHVNGDDPEAVAFVTGLAVEYRQTFKKDVVIDI
                     VCYRKLGHNEADEPMVTQPLMYKKIAKHPGTRKLYAEKLVQLGVCKAEDPDQFIKDYR
                     AALDKGELVTNPVLSDFKRQFSQDWSPYVNKKYTDKCDSRVPMAELKRLSKALTTTPE
                     GFVLHPRVQKIIDDRKAMGEGKLALDWGMGENLAYASLLVQGYGVRLSGEDVGRGTFF
                     HRHAALHDQNRDQWHDGTFHPLKNIREGQGPFYCYDSVLSEEAVLAFEYGYASAEPNQ
                     LVIWEAQFGDFANGAQVVMDQFISSGEAKWGRQCGLVMMLPHGYEGQGPEHSSARIER
                     YMQLCAEMNMEVCIPSGPSQIFHLLRRQALRKLRKPLVIITPKSLLRHKDAVSSLDDL
                     SKGTFQTVIGEVDDLDAKKVKRVLLCSGKIYYELLAHRRANNIDDIAIVRMEQLYPFP
                     ADAFSAEVNKYPNAKEVVWVQEEPRNQGVWYWLASRHHLDTNLSDDHKFLLVARPAAA
                     SPAVGYYAKHNAQQKAVIENAFGEINA"
     gene            19131..20315
                     /gene="odhB"
                     /locus_tag="PLH85_07145"
     CDS             19131..20315
                     /gene="odhB"
                     /locus_tag="PLH85_07145"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNB64373.1"
                     /translation="MLIEVKVPQLSESVSEATLVSWHKKEGDTVSRDENLIDIETDKV
                     VLETPAPADGVLVKIVKNGGDSVTSGEVIATIDTEAKAAAAPAAAAAPAPAAAPAAAA
                     PAAAVSAASPSARKILDEKGIAAADVAGSGRGGRVTKEDAVAAQPKAAAAAPAVAVPT
                     GDRVEQRVPMTRLRARIAERLIQSKNENAILTTFNEVNMAPIMALRKQYGDKFEKAHG
                     VRLGFMGFFVKAAVAALKKFPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDV
                     DQMSIADIEKKIAEFGQKAKDGKISIEELTGGTFSISNGGVFGSMMSTPIINPPQSAI
                     LGIHATKDRAVVENGQVVVRPINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLIL
                     DV"
     gene            20394..21815
                     /gene="lpdA"
                     /locus_tag="PLH85_07150"
     CDS             20394..21815
                     /gene="lpdA"
                     /locus_tag="PLH85_07150"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB64374.1"
                     /translation="MAKQFDVLVIGGGPGGYVAAIRAAQLGFSTACAESNPYADPKGE
                     PRLGGTCLNVGCIPSKALLHTSHLFEEAGHAFEEQGISVGKPKIDVPTMIGRKSKIVE
                     QLTQGIKGLFKKNKVTFLAGHGSFVAPIDGGWQVKVGEEVVEAKQVIVATGSTARHLP
                     GIPVDNKLVCDNIGALDIDAVPKRLGLIGSGVIGLEMGSVWNRLGAEVTILEAMDSFL
                     AAADQDVAKEALKLFTKQGLDIKLSVKVGAVTVGKKSVTVEYETKDGAQKAEFDRLIV
                     SVGRVPNTAGLNAEAVGLKIDERGFVDVDGHNRTNLPGVWAIGDVVRGPMLAHKAMEE
                     GVAVAETMAGQKGHTNFDTIPWVIYTSPEIAWVGKTEQQLKAEGVAYKVGKIPFLANG
                     RALGAGAPAGFVKMLADANTDRVLGVHIIGANASELIGEAVVTMEFAGASEDLARICH
                     AHPTLSEVVHEAALACDKRPLHF"
     gene            21924..23036
                     /gene="zapE"
                     /locus_tag="PLH85_07155"
     CDS             21924..23036
                     /gene="zapE"
                     /locus_tag="PLH85_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNB64375.1"
                     /translation="MPHRILNVSEKGMLDAYEGLLRARGYKSDPAQRAAAQRLQGMYS
                     ELVAFKAARRGAIRKLLSRPKMPRSVYFWGGVGRGKSFLMDCFFDSVPYQRKRRVHFH
                     AFMQEVQNDLKNLNHEPDPLQKVADRIARQTRLLCFDEFHVSDIADAMILGRLLDALF
                     QRGTIFVMTSNYPPDGLYPNGLQRINFLPTIDMIKRRFDVVEVDHGTDYRLRSLEKME
                     AFLVPGDDVADKRLASDFAQIAGTPGEGGEIEVLERTLKVVRQAPGVIWFDFATLCGG
                     ARSQNDYLEIAREHHTLILSRVPKMNAGMASEARRFTWLIDVLYDHRVKLLVSAECEA
                     YDLYREGHQANEFQRTVSRLMEMRSREYLAEAHRVD"
     gene            23044..23859
                     /locus_tag="PLH85_07160"
     CDS             23044..23859
                     /locus_tag="PLH85_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64376.1"
                     /translation="MGRGRFTVLRGAMAAIVGLGVPAVPAQAPVGRLEIIPLRHRPAD
                     QIVAAIRPLVAPGGALSAMGDKLVLRTSPANLAELRRAIEALDTPIRRLVISVRQGDV
                     RDFAMADGGVGGIFRPGDSRVVIRGDARQGSAREQIDQQVEVIEGGQALIRVGQSVPL
                     RMRQGYATPDGGWVTADSIALVDVGSGFYAAPQLVGEQVRIEIRPEQARLDAGGQVES
                     ARLATTVSGPLGAWIPLGGSTTESTRNAAGLSGAAQGRETLSRQVWLRVDALD"
     gene            23864..25822
                     /locus_tag="PLH85_07165"
     CDS             23864..25822
                     /locus_tag="PLH85_07165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNB64377.1"
                     /translation="MDIDTAFAADGPLAAAIPGFAPRPQQLEMARQIADTLRANRVLV
                     AEAGTGTGKTFAYLVPALLNGGKVILSTGTKTLQDQLFNRDLPTVRGALKVPVAVALL
                     KGRANYVCHYHLARNLVDGRFQSPQDAVHLRAIGRFLEITRSGDKADCPEVPEDAPAW
                     MYATSSRDNCLGQDCPNIKECFVAAARRAAMEADVVVVNHHLFFADVMLRDEGMGELL
                     PSCNAVIFDEAHQLPETASLFFGESVSTAQVLELARDTRSETVAAARDCVDLIEATRS
                     LEKAARDLRLAVSAENARFGFAQLADNPGFHEAVAHLDDELGAFRILVESQAERSEGL
                     AACLRRTQEIQERLTRWRAPENTDWVRWVEVFSQTLALNATPLSIASIFKRQLDGHPR
                     AWIFTSATLAVGPDFGHYCRELGLHWLEPPPDTALWGSPFDYPQQAILYAPQDMPDPN
                     SPVYPEAVARVAYPLIRAAQGRTFVLCTALKAMRRIHEVIADKLAAEQLDLPLLLQGE
                     GSRSELLARFRRLGNAILVASQSFWEGVDVPGDALSVVVIDKLPFAPPDDPVLAARIE
                     HLQKSGRNPFMEYQLPRAVINVKQGAGRLIRTERDRGVLAICDPRMLSKPYGRRVWQS
                     LPPMRRSKVEAEVVAFLEDLPPPAAGGC"
     gene            25907..27262
                     /locus_tag="PLH85_07170"
     CDS             25907..27262
                     /locus_tag="PLH85_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64378.1"
                     /translation="MTTRLRSADPEPADRAHSTGTAGCGGRAETLNATCQCITLDRPA
                     LREALARDPRDGALTALLAESRPHLFSDAMAFVAQAHLEHMAEVVAAIERVVAQPAWQ
                     AAVLADAPVAARRPGAAQGVFLGYDFHLGPDGPRLIEINTNAGGGLLSAVLGRAQRAC
                     CAEVGSLMRAAVPTGTDDAETAFLAMFRAEWQAERGDAPLRRIAIVDDRPGDQYLLPE
                     FLLFQRLFERHGIAAVVCDPAELRCERGCLWHDGQRVDMVYNRLTDFALDEPAHAALR
                     AAWNTDAAVITPHPRAHALYADKRNLVRLSDPDLLRGLGVDAETQAVLGAGVPRTEAV
                     TPDRAEALWAARRGLFFKPASGFGSRGAYRGDKLTRRVFAEILAGDYIAQALVPPSER
                     RVTVDGAAVDLKMDLRNYVYGGQVQLVTARLYRGQTTNFRTPGGGFAPVLAVPCDDAR
                     G"
     gene            27267..27758
                     /gene="mobB"
                     /locus_tag="PLH85_07175"
     CDS             27267..27758
                     /gene="mobB"
                     /locus_tag="PLH85_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNB64379.1"
                     /translation="MKVFGFAGWSGSGKTTLMERVIPLLTGRGLKVSVIKHTHHRFDL
                     DQPGKDSWRHREAGAAEVLLISERRWALMHELRDEPEPDLAAQLARMSPCDLVLVEGF
                     KHLGLPKLEIHRPAHGRPLLHLSDPAIVAVATDGALATDLPCLNLNDPAAVAGFIVDH
                     LEL"
     gene            27760..28983
                     /locus_tag="PLH85_07180"
     CDS             27760..28983
                     /locus_tag="PLH85_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNB64380.1"
                     /translation="MSSTLRSYEDVRDALIALARPVVETEVLPTAEALGRVLADAVVS
                     PLEVPPLDNSYMDGYAVRIADLPAPGTRLPVSQRIPAGSVGSRLQPGTAARIFTGAPI
                     PAGADAVVMQEFCSPDGDGVVIDHLPRPGEWIRRAGEDIARGQTILHAGDRLTPQRLG
                     LAASVGAAHLTVRRRLRVALFSTGDELVMPGEALPPGAIYNSNRFTLRGLLEGLGCQV
                     DDRGIVPDRLDATRAALRGAAEVSDLILTSGGVSTGEEDHIKPAVQAEGRLDSWQVAM
                     KPGKPVAFGHVNGAAGTAGAAFIGLPGNPVSSFVTFLVLVRPFILRAQGVREVLPRAL
                     PLTAAFDLPRPDRRREFLRARLGADGRVEIHPNQGPAVFTSTVWAEGLVDNPPGHVVQ
                     AGDRVHYIPYSELLS"
     gene            28980..29234
                     /gene="moaD"
                     /locus_tag="PLH85_07185"
     CDS             28980..29234
                     /gene="moaD"
                     /locus_tag="PLH85_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNB64381.1"
                     /translation="MSVKVLYFASLRETLGYSHETLTLPTQIATLGDLRTHLARRGGV
                     WEALGEGRNVRAAINQEMAGPERPVADRDEIAFFPPVTGG"
     gene            29236..29724
                     /gene="moaE"
                     /locus_tag="PLH85_07190"
     CDS             29236..29724
                     /gene="moaE"
                     /locus_tag="PLH85_07190"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HNB64382.1"
                     /translation="MSTTGFRIQVQEADFDTGAEIAALSAGRDDVGAVASFVGLVRAD
                     KSACQTGHVSAMTLEHYPGMTEKALEAIVDEAAGRWSLQGVRVIHRFGRLLPGERIVF
                     VGVASSHRGDAFAACEFIMDYLKTRAPFWKKEDTPAGGRWVDAREADDSAAARWAQPG
                     GD"
     gene            29876..33037
                     /locus_tag="PLH85_07195"
     CDS             29876..33037
                     /locus_tag="PLH85_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB64383.1"
                     /translation="MWIVRIALQRPYTFIVMALLIVLATPLVLLRIATDVLPEINIPV
                     ISVIWTYTGMSAQEMAQRVTGPNERLLATTVNDVEHFESQSYAGIAIIKLFLQPKASV
                     EAALAQIASTEGGALRQLPPGITPPLVIRYTASAIPVVQLGISSASLPEQVMFDTAVN
                     TLRPRLVSIPGVALPFPYGGKSRVVSVDLDLPALQAKGLAPLDVVNAINAQNLILPGG
                     TAKIGATEFAVKMNGSTRSVEELNDLPVRLGSGAAVYLRDVAVVRDGFSPQTNIVRQD
                     GKRGVIQSVLKNGGASTLQIVDNLRTALADALPSLPEDIRVTPLFDQSVFVRAAIGGV
                     VHEGLIAACLTAAMILLFLGNWRTTCIVAISIPLSVLASILTLYALGETINIMTLGGL
                     ALAVGILVDDATVTIENIERYLHLGHDLETAILEGAGEIAVPALVSTLCICIVFVPMF
                     FLTGVARYLFVPLAEAVIFAMLASYLLSRTLVPTLALLFMKGQHGGGAGGWLQPLYTR
                     FDAAFERLRGGYIVLLSVLLPRRRRFIAAFLAVCALSMGLYAVLGRDFFPAVDAGIVR
                     LHFRAPSGTRIEETARLADRIEAALRELIPADQITTLVDNLGLPNSGINLSYSNAGTI
                     GTLDGEIQVALAPGHGSSANLVERLRDALPPRFPGIEFFFQPADVVTQILNFGLPAAI
                     DVQISGLRMAENYRVAAQLEKAVRRIPGAVDVHIHQRFDLPTVNLAMDRNRLQQLGLS
                     AANVAQNVLIPLSGSFQTAPSFWLNPRNGVSYNITVQSPQYRIDSLDSLLQIPVAGSA
                     GTPGQLLSNLVQVGSGREPAIVSRYNIEPAVDLYVSVHGRDLGAVVADVEREVEAVRG
                     LLPKGSRISLQGQARTMNSSFIGLGIGVVMAVVLVYLLIVVNFQSWVDAFIIITALPA
                     ALAGIAWMLFLTGTPLSVPALTGAIMTVGVATANSILVVSFARQRLLAGAPPLSAALE
                     AGATRLRPVLMTALAMIMGMLPMAIGLGEGGEQNAPLGRAVIGGLLWATLSTLVFVPL
                     VFAAVHRGFARRTARRAALAGSI"
BASE COUNT         5492 a        10939 c        10961 g         5784 t
ORIGIN      
        1 caggccgccg aagaagggca gggtgtcggc atagacgccg ttgctctgca ccggggtgag
       61 gatgtcgtcg ttgctgaagc cgttggcctt gcaggagttg aagtcttcca gaccgtaggc
      121 gggcgccgag tggacgatgc cagtgccggc gtcgaggccc acgtagtcgg cgacgaagac
      181 cggcgacagg cggtcgtaga aggggtggcg gaaggcgatg cggtcgaggg agcggccgtc
      241 ggtgcgggcg atcacgcggc cttccaggcc gaagcgcttg aggctggcgt cggccagttc
      301 ggccatcagc acgagcaggc cgcggggtgt gtccaccagc gcgtaggcgt gctccgggtg
      361 cacgttgagg gcctggttgg cggggatcgt ccacggggtg gtggtccaga tcaccgccca
      421 ggcggtcttg tccgcgggca gcgcgggcag gccgaaggcg gcggcgagcc tgtcgggttc
      481 ggcgcagggg aaacccacgt cgacggccgg gctctgcttg tcctggtatt ccacctcggc
      541 ctcggcgagg gcagagccgc agtcgaagca ccagttcacc ggcttgaggc ccttgaacac
      601 ccagccgcgc ccgaccatct cggccagggc gcggatctcg ccggcttcgt tgccgaagtc
      661 catggtgcgg tagggattgt cccagtcgcc cagcacgccg aggcggatga agtctttctt
      721 ctgttcctcg atctgctcgg cggcgtaggt gcggcacagc gcgcggacct tgtcggcggg
      781 caggttcttg ccgtgggtga cctcgatctt gtgctcgatg ggcagaccgt ggcagtccca
      841 gcccggcacg tagggggcgt cgaagccggc cagggtcttg gagcggacga tgatgtcctt
      901 gaggatcttg ttgagggcgt ggccgatgtg gatgctgccg ttggcatagg gcgggccgtc
      961 gtggaggacg aacttgggcc ggccggcggc ggccttgcgg atcttctggt agagcttgcg
     1021 ctgctgccag tcggcgatcc agccgggttc gcgcttggcg agatcgcccc gcatcgggaa
     1081 gggggtgtcg ggcaggttca gggtcttgcg cagttcggac atggcggcgg ggatgtcaga
     1141 ggggattccg ggcgaaatgg gcgcgggcct ggtcggcgtc gcgggcgatc tgggcggtca
     1201 gggcgtcgag gctgtcgtag cgttcttcgg cgcgctgcca ggcgaggaag ttgacccgca
     1261 ggtgggcatc gtagcagtcg ccgtgccagt cgaagagatg gacttcgagg ctgggccggc
     1321 cggcgtcgtt gatggtgggg cgcacgccga tgctggccac gccgggccag ggccgctctg
     1381 caacaccgtc cacgctcacc gtgaagatgc ccatcagcgg cgggctgcgg tgcttgagct
     1441 ggatgttggc ggtgggaaag ccgatcgtgc ggccgatctt gtcgccgtgc aggacgcgtc
     1501 cgctgatgct gtaggggcgt ccgagcaggc gtgcggcgcg gggcatgtcg ccggcggcaa
     1561 gggcctcgcg cacggcggag ctggacacgc ggccgtcggc gtccgccagg gtgggcatgg
     1621 cctcgacggt gaagccggcc cgttcgccgg tggcgcgcag cagggcgaag tcgcccttgc
     1681 ggccgcggcc gaagcggaag tcgtcgccga tcagcaggtg gcgcacgccg aggccgtgga
     1741 tcagcacgtc ttcgatgaag gcctccgcgc tcaggccggc gaaacgctcg ttgaagcgtc
     1801 cgatcacgat gcggtccacg ccgtgttgtt cgagcaggcc gaccttttcc cgcagggagg
     1861 tgaggcggac cgggcggacc tcgtgggtga agtattcccg cgggtggggc tcgaaggtga
     1921 gcacgacagc gggcagaccg gtctcccgcg ccttggcggc gagccgcgcg agcagcgcct
     1981 gatggccgcg atgcacccca tcgaagttgc cgatggtgag cacgcagccg tgttcggcgt
     2041 ggtcggggat tccgcggagg acctgcatgg ggcgttcggg caaaggcgcg attatagcgg
     2101 ctcggggcgg cccggcgaag ccgccgccag cgcgtgcgtt caggcgtcct ggccggcccg
     2161 ttcccgatga aagcgggcga cgtagtccgt cagccggccg tgggcgatgg cgtcgcgcag
     2221 ttcgcgcatg agctgctggt agtagtgcag gttgtgaatg gtcgccagac gcgcgccgag
     2281 gatctcgttg aggcggttga ggtggtgcag gtagccgcgg gagaagtgcc tgcaggtgta
     2341 gcagtcgcag gagtcgtcca gcgggcgcgt gtcggccttg tggcgggcgt tgcggatctt
     2401 gatgtcgccg tggcgggtga acagccagcc gttgcgggcg ttgcgggtgg gcatgacgca
     2461 gtcgaacatg tcgatgccgg cctgcaccgc gaagacgatg tcctccggcg tgcccacgcc
     2521 catcaggtag cgcggcgagt ccttgggcag gcgcggcgcg gtgtgatgga ggatgcgctg
     2581 catgtcttcc ttgggctcgc ccaccgacag gccgccaatg gcatagccgt ggaagccgat
     2641 ctcggtgagc ccggccaggg attcgtcgcg cagggattcg tacatgccgc cctggacgat
     2701 gccgaagagg gcgttggggt tctcgaggcg gtcaaattcg tcccgcgaac gccttgccca
     2761 gcgcagggac aggcgcatgg agtcgccagc ctcggtctcg ctggcggggt agggcgtgca
     2821 ttcgtcgaag atcatcgcca cgtccgagtt gagtacgcgc tggatgcgca tggactcctc
     2881 gggcgtaagg aagagcttgt cgccattgac gggcgaggcg aagcgcacac cttcctcgct
     2941 gatcttgcgc agggcaccca gggagaacac ctggaagccg ccggagtcgg tgaggagggg
     3001 gccgtcccag gccatgaatt ggtgcaggcc gccaaaggcg gtcatgacgt cgaggccggg
     3061 gcgcagccac agatggaagg tgttgcccag gcagatctgg gcgccgatgt cgcgcaggtc
     3121 ctggggcgag atgcccttca ccgagccgta ggtgcccacg ggcatgaagg ccggggtgtc
     3181 cacggtgccg tggttgaggg tgagtcggcc gcggcgggcg gcgccgtcgg tggcgaggag
     3241 ttcgtatttc atgcgggctt ccgggtcagg aacatggcgt cgccgtagct gaagaagcgg
     3301 tagcgctgcg ccacggcgtg ggcgtaggcg ctgcggaggg tgtccatccc ggcaaaggcc
     3361 gagaccagca tcagcagggt ggacttgggc aggtggaagt tggtgatgag ggcgtctgcc
     3421 acgcgaaatt ggtagccggg caggatgaag aggtcgctct cggcctggcc ggcacgcagg
     3481 ctgccgtcgc gggcagcgga ttcgagggcg cgcaggctgg tggtccccac gcagatcacc
     3541 cggccgccgg tggcgcgggc agtgtcgatg gcggcgacgg tggcgtccgg cacgtggtaa
     3601 gcctcggtgt gcatgcggtg ctgggtcaga tcctgcacgc gcaccggctg gtaggtgccg
     3661 gcgcccacgt tgagggtgac ggtggcgctg cgcacgccct gagcggcgag ctgggcgagg
     3721 agggcgtcgt cgaagtgcag cccggcggtg ggcgcggcta ccgaaccggg atggcgggcg
     3781 tagacggtct ggtagcggga ttcgtcggct tcgccggccg cccgttcgat gtaggggggc
     3841 agcggcagac ggccgtgacg ttccagcaac gtcaccaggg gcgcatgcgg cgggaagcgc
     3901 aggtggaaga agtcgccggc gcggccgagc acgtccacgt cgagggcgtc ttccaggcgc
     3961 aggcgggtgc cggctttggg cgatttgctg gcgcggatct gggcgagggc ttcgtgttcg
     4021 tccagaggac gctcgatcat cacttccacg cgtccgccgc tgtccttcac gccgaacagg
     4081 cgggcgtgaa gcacgcgggt gtcgttgaag acgaggagat cgtccggccg cagcagggcg
     4141 gggagatcgg caaaatgcag gtcggcaagg ggctcggcca cgtgcagcag gcggctgtcg
     4201 gtgcgccggg gcaggggcgc ctgggcgatc agttcggggg gcagcgggta gtcgaaatcg
     4261 tcgagggtca aggccatgtt gatgcagtaa gtcgattcgg ggataatgcg cggctccctt
     4321 gccgggatgg cggaatcggt agacgcagcg gattcaaaat ccgccgccga aaggcgtgag
     4381 ggttcgagtc cctcttccgg caccacaggc tttttgccgt cgggtagtca tgcggtgcgc
     4441 gacgcaccga cgtcgggcag cgcccgcgcg tatcggacga agtcgcgcat ggtagctgtc
     4501 cggggcgtca tccgcaaacc cggtgtcggc cggggcggat cattgccggc aacgctggcc
     4561 cggctgtctg gcgtgatgct tgctctgatt ctggcctgcg gcgcgcgatg atgtcgttcg
     4621 ccactcccat cgatctttcc ggaccttctc ccatggctct cgatccccag gcacgggctc
     4681 tgctcgacat ggtctatcgc gtgggtgccc cgcgcttcca cgagctcgac gtgcatcagg
     4741 cccggcattc cttcgagaag ctgctgttcg ccctgcgtcc cgaggcgccg cccgtcgcgt
     4801 cggtgacgga agtgcccatc ccccgcgccc acgcccgcga cggcgtgatc atggcgcgtc
     4861 tgtaccgtcc gctgaattcc cacgccgacg aggctctacc gatcaccttc tattttcatg
     4921 gcggcggctg gtgcgtgggc aacgtggcga gctacgacgt gctggggcgg caactggcca
     4981 atctgtcggg gggcgcggtg ctgtcggtgg attacgcgct ggcgcccgag catcccttcc
     5041 ccggcgcagt ggtggacgcc cagcgggcgg tggattgggc gcttgcgaac gcggtcctgc
     5101 tgggcgtcga tccggcgcgt tttgccctgg ccggggacag tgccgggggg accctgtcgg
     5161 ccgtgcttgc ccaccagatg cgtaacgccc gggtccatgc cgcccggctg cagctgctca
     5221 tctatccgtg tgtggaaatc ctcagccagc gaccgtcccg caagacctac tcgaacggct
     5281 acttcctcga caacgagagc atggcctggt tcttcgacaa gtatctgccc aatgcggaag
     5341 actggtccga ctggcgcgcc tcaccgctgc tcgcaccgcg cttcgatgac ctgccgccgg
     5401 cagcgatcat caccgccggc tgcgatccgc ttacggacga ctgcctcgcc tacgctgacg
     5461 cactgcgcgc ggccggtgtg cccgtttcgg tgcatgggtt cgacgggatg gtgcatggct
     5521 tcttcaccct cgggaagttc tttccgcagg ccaatgaggc cgtgacgcgg gcgggtgagg
     5581 ctttgaggga tgccttgaag gcgtaggacg cctgccgggg gtgcaggctc aaaggcagac
     5641 gaagtcgacc acctgggggc tgttggcgca gctgcggccc agcgagaagt ggccgcgccc
     5701 gcgcaggacc acttcttcgc gggacagcag gaactccttc tcgccgtcga tctgtacgaa
     5761 ggtgccgttg ctgctgcggt cgatgagcac gaacttgtcg ccgcgcagtt cgatctcggc
     5821 atggcggcgt gacgactggg tgtcggccac caccagatct gctgcggtgt cgcgaccgat
     5881 gcgcgcgctg ggccgcgtgg cattgagcag gagcgtcttg gagtccaggt acagacgcag
     5941 ttccggttcg gtggcgagca ggaaatgatc gctctggaca atggtgagct cacccaccgc
     6001 ctcgcacatc aattcgcaga cctcgaccgg ccgcgacagc ccccggatcg cccggggcgg
     6061 cagggtatgc agcaccgagc gccactcggg gcccaggcgg cgcgccgtct ctgaggaggt
     6121 gatcgccttt ccgggcgagg ccagctccgc caggcgggcg gcgaagttga cggtttcgcc
     6181 gaagacatcc gtgccggcca cgacgaccgg cccgaagtgg aagccgatgc gcaaggccag
     6241 cttctggtca ccggccgcgg gaagttcctt gacggtctgg tggatcagca gcgtggcatc
     6301 ggctgcactg tcggcgtcgc ggaagaccgc catgaggccg tctccggtgt gcttgacgac
     6361 acgtccctgc ttggtttcaa cggcgatgcg catgccgcgg atgcaacgat ccacctgttc
     6421 gaaggctgcc ttgtcgccga cgcgctcgta gagctccgtg ctgccgacca gatcggcgaa
     6481 caggactgcg tactcggaac tgggaggtac agcttcaggc atctcgattc ggacgtttgg
     6541 gtgacacaga attttacgga atccgtgaac aattccgcag tttcaaaggt accaaatttt
     6601 gatggccttg gctgctccgc ttgtcggcgt tgatgctgcg acgaaaggag gcggcaaggg
     6661 agatggcact tccttgatct aatctgcggc gcaacgtcgt gggcggtggg ataatgcgcg
     6721 cctacgcctg cggtacgcgc atcgtccgcg gctctttacg tcttgaaagg gcttcatgaa
     6781 acgagttgat gatttccggc tgcgcttcgg caggcaggag tacgtgccga tcatgatcgg
     6841 cgggatgggg gtcgacatct ccaccgcgga tcttgcgctg gaagcggcgc ggctgggggg
     6901 ggtcggccac atctcggatg cgatggtgaa gacggtagcc gatcgccgtt tcaagacgaa
     6961 gtatgtgaag gaaaaacttc agcaatacaa gtacaacgtt gcaaaccctg acaaatcgga
     7021 ggtccagttc gatctgggcc acctggccga agcgacccat ctgcacgtgg ggcgtaccat
     7081 ggaggcgaag cgcggccagg ggctgatctt cgtgaactgc atggaaaagc tcacgatgaa
     7141 cgcgcccaag gaaaccctca aggtgcgcct cactgcggcc atggacgcgg gaatcgacgg
     7201 catcaccctg gcggcggggc tgcatctggg ctcgtttgcc ttgatcgagg atcatccccg
     7261 cttccgcgat gtgaagcttg gcatcatcgt ttcgtccctg cgcgcgctcc agctcttcct
     7321 gcgcaagagt gcccgcacgc agcgtctgcc tgattatgtg gtggtggaag gcccgcttgc
     7381 gggggggcac ctggggttcg gcatggactg ggcgcagtac gatctggcga ccattgttgc
     7441 cgagatccgc gactggctga aggtcgagca gctcgacatt ccgctgatcc cggccggcgg
     7501 catcttcacc ggcagtgatg cggcggcctt tctggaggcg ggggccggcg ccgtacaggt
     7561 cgccacgcgt ttcacggtgt ctcaggaatg cggtctgccc gacgcagtca agcagcagta
     7621 tttccgggcc gatgaggatg acattgaggt gaatcagatc tcgcccaccg gctatccgat
     7681 gcgcatgctc aagaacagtc cggcgatcgg ttcgggcatc cgccccaact gtgaggccta
     7741 cggctatctg ctcgacgcca aggggagctg cgcctacatc gaggcctaca accgggaact
     7801 ggcgctgcat ccggccgaga agaaactttc ggtgctggac aagacctgcc tgtgcaccca
     7861 catgcgcaac ttcgactgct ggacctgcgg gcacttcacc tatcgcctga aggatacgac
     7921 ccggctcgac gagaatggtc agtaccggct actgactgcc gagcacatct tccgcgacta
     7981 ccagttcagc accgaccaga agattgccct gccggattga gggcatcggc cccggcggat
     8041 gccaatcggc ccgatcgctt ccggctcgcg ccagggagtg atcgggccga tgtcctgtgg
     8101 gcagtgctca gaagaagcgc gcgagggcgt catcgggcag attcatgccg gtggcgcggg
     8161 cacgcttgag gagctcccag taatagcggt aggacgcccg gtcgtggagc ttgccggcgt
     8221 gctggatcgg tccccagccg gcatcctggg cacccgcgag gatggcggtc gcttcctcga
     8281 cctcggaaaa atccgggcgc atggcttcga cgatggggag gatctggttg ggatggatgc
     8341 tccacatgcg caggtagccg aattcccgac gggcgcgttc ggcgtcgcgg cggatgtagg
     8401 ccagatcctt gagttcggtg gtgacgttgt gggacggcac gacgccgtgg gccagggcgg
     8461 cggcggcgat gtcgcacttg gcgcggatga tcagcggatg ctcgaactgg ccagggctgc
     8521 gcatggcggc gcccggaatg gcgccatggt gcccgctgac gaagtccatc aggccgaagt
     8581 cgagggattc gacccggtcc agggcggcaa tctcccaggc atcacgcagt gcaccatggg
     8641 tttcgatgag gacgtgtacc ggaatggcct gctcgatgcc gtggcgttcc tcggcgctgc
     8701 ggagggcgtc gatctggcgc gcggcatccg caacgctgcg gggcttgggc agcgtcagga
     8761 aggccaggcg ggcaccggct tcgcccacca ggatgtccac gtcctgttcc cagtgggggt
     8821 gggtgatgtc gtggatgcgg gcccccacgc ggccgaagcg gttgtcgccg gacatgacga
     8881 tgctggcggc catgcgggca tgttcggcct cggcgccagc gtgggcgcca tcctcgcagt
     8941 cacaggtgat gtcgaagagc ggaccgatct cctgctgcag ggcgagggcc ttgcgcatca
     9001 gtttctcact gccggcatag tgatcaacgg ccggcaggac ggggaagggc ttttcgccct
     9061 ggaagaggac ggaatcgggg tggtggtcgg cggtcatggc gtgcggaaag agggcatctg
     9121 agccgccgga ggataacaaa aaacgcgggg gcgtttccgc ccccgcgttc tgcacagctg
     9181 ggatgcagcg aggatcagcc gagcagatcc ttgacgccgt cacgctcttc gaggagttcg
     9241 ttgagggtgt tggtcatctt gctgcgggag aactcgtcga tctccagacc ctggacgatg
     9301 gtgtattcgc cattgacggt ggtcaccggg aagccgtaga tgatgccttc ggggatgccg
     9361 taggagccgt cggacggaat gcccatggtg acccattcgc cgttggagcc gaggacccag
     9421 tcacggatgt ggtcgatcgc cgcgttggcg gcggaggcgg cggaggacag gccgcgagct
     9481 tcgatgatgg cggcgccgcg cttgccgacg gtgggcagga acacgtcgcg gttccaggcc
     9541 tcgtcgttca ccagggcggg caccttgtcg ccgttggacg tggcgaagcg gtagtcggcg
     9601 tacatcgtgg gggagtggtt gccccacacg accaggttct tcaggctgga cacgtcacgg
     9661 ccagtcttgg tggccagctg ggacagggcg cggttgtggt ccaggcgcag catgccggtg
     9721 aagttcttgg cctggacgcg accgtacttg acggcgattt ccttggcgat ataggcattg
     9781 gtgttgcagg ggttgcccac caccagcacc tttacgtccg gatcggcgta ctggccgatg
     9841 gccgcgccct gaacggtgaa gatcttggcg ttttcggtca gcagatcctt gcgctccatg
     9901 ccggggccgc gggggcgggc gccgaccagc agggcaacct gggcatcctt gaaggcaacg
     9961 ttcggatcat cggtggcgat gacgccagcc agcagcggga aggcgcagtc ttccagctcc
    10021 atgatcacgc ccttcacggc cttctgggcc tgcggcaggt cgagcagttg caggatcacg
    10081 ggctgatcct tgccgagcat ttcaccgctg gcgatgcgga agagcaggct gtaaccgatc
    10141 tggccggcag cgccggtgac agcaacacgg acgggggctt ttgccattat tgatactccc
    10201 tcaggaagga aagaagtttt atcggactga tgcttcgaga tttcgcgtcg ccgtagcgcc
    10261 gccgcgaaga tgggtcgagg ttgcgatcat cgccggatcg gtcaacatac gaatcgtagg
    10321 aacattttga tgacctgtca atttcgatgt cttatgtctt atataagata taagacagcc
    10381 tatggactgt cttttgaatt tatgcttcaa taagtccatg cgacaggact cgcccacttt
    10441 cagccccctt taccgtcaga tcaagaatct gatgatgaat gcattggagt cggctgaatg
    10501 gcggcccggg caggccattc ccagcgaaca ggagctggcc ctgcgattca gcgtgagtca
    10561 gggaaccgtg cgcaaggcca tcgatgagat ggctgccgag aacctgttgg tgcgcaagca
    10621 ggggaagggt acgtatgtcg cgtcgcacaa cgatccgcgt gcgctgttcc gcttcctgcg
    10681 gcttgtgcca atcgacggtg agctggctca tccccaaagt atcccccttg attgctggcg
    10741 tgccaaggcc ggcaatgagg ccgcgcgaat gctcggaatc gagctcggcg cgccggtcac
    10801 gatcctgcgt cgcctcctca agtttggcgg gcgaccggtg gtggtggatg agatctatct
    10861 gccgggcgag gtttttcatg gcctgacgat ggaagtcctg cagggttcga cagggtccct
    10921 gtacgggctg ttcgaggccc ggttcgggat ccgcatgatc cgtgccgagg aacgcattcg
    10981 cgcagtcgcg gctgatcgca tggtgggcga gacgctcggc gtggcggaag ggacgccgct
    11041 cctgtcggtc gagcgcgtca cctacaccta tggcgacagg cccgtggagt ggcggcgcgg
    11101 cttgtattcg actgcagacc attattatct caatgaactg agttgaaggg cgggtcgcaa
    11161 ggcccggtct gtcaggggct ggtaagggta tatacttaca attttgttgt gtgggataag
    11221 agcggcgctc agccgctatt cgctagaggg agtatctatc tatgacagag gtgactttga
    11281 agaaacagcg cccgaagcat ctggcgctga acgagatcag gcttcccctc cccgggaaag
    11341 tctcgatcct gcaccgcgtc agtggcgcgg ggctgttcct gatgctgccg gtattgctgg
    11401 cgctgttcgg tgcgagcgtc ggttcgcctg agtcctatgc ggcctacaag gacgtggtga
    11461 gcaatgtgtt cgtcaagctg ctcctggtcg ggttgctgtg ggcgttcctg catcatttct
    11521 gtgcaggtat ccgtttcctg ctgctggacg tgcatgtcgg tgtcgacaag gaatctgcgg
    11581 tcagcagtgc ccgcaccgtc ctgatcgtca gcctcgccct cactgtcatc ctggtggcca
    11641 aactatggta aagcccatta ttgtcggcgc ccattacggt ttccgtgact ggctcgccca
    11701 gcgcattacc gccgtcgtca tggcggtcta ctccattctg tttgcggtgt ccgccctggc
    11761 gactttggat ggcacctatg aaagctggcg cggccttttt agcggcggct tcatgaagtt
    11821 cgcgaccttc ctgttcttca tgtctctgtt ctaccacgcg tggatcggcg tacgcgacat
    11881 cttcatggac tacatcaagc ccacgggtct gcgtctcacg ctgcattccg tcgtcgctct
    11941 tctgctcatc ggctacgccg gctgggctgc tcagattctg tggaggctgt aagtgaaagt
    12001 cgcaaagcgt acatttgatg cagtaatcgt cggcgcgggt ggcgccggtc tgcgggcggc
    12061 gctccagctt tccgaagcgg gcatgaagac agccgtgctg accaaggtgt tcccgacccg
    12121 ttcccacacc gtggcggcgc agggcggcgt ggccgcatcg ctgggcaatt ccaccgagga
    12181 caactggcac tggcacatgt acgacaccgt caaggggtcg gactggctgg gcgaccagga
    12241 cgccatcgaa ttcatggtca aaaaggccaa tgaagtggtg gtcgagcttg agcactacgg
    12301 catgcccttt gaccgtaccg acaacggcaa gatctaccag cgccccttcg gtggtcattc
    12361 ccagaactac ggtcagaccc cggtgtcccg ctcctgtgcg gccgctgacc ggacggggca
    12421 tgccatgctt cacgcgctct atcagcgcaa cgtacgggcc aacacccagt tcttcgtcga
    12481 gtggatggcg ctcgatcttc tgcgcgatgc cgaaggcaat gtccttggcg tgaccgcgat
    12541 ggaaatggaa accggcgagg tctcgatctt ccattcccgc gccaccatct tcgcgacggg
    12601 cggtgccggc cggatctact acagttccac caacgccttc atcaacaccg gtgacggtgt
    12661 cggcatggcg gcgcgtgccg gcattccgct ggaagacatg gaattctggc agttccaccc
    12721 gaccggcgtg gcgggtgcag gtgtgctgat caccgaaggt gtgcgtggcg aaggcggcat
    12781 cctgcgtaac gcgtccggtg agcgcttcat ggaacgctac gcgccgaacc tgaaggatct
    12841 ggcctcccgt gacgtggtgt cccgttccat ggtcaccgag atcaacgaag gtcggggttg
    12901 cggttctgac aaggatcacg tgctgctgga catcacccac ctgtcgcccg agaccatcat
    12961 gaagcgcctg ccgggcattc gtgagatctc catccagttc gccggagtgg atccgatcaa
    13021 ggccccgatc cccgtggtgc caacctgtca ctaccagatg ggcggtatcc cgaccaatta
    13081 caagggtcag gtcgtggtgc cccaggacgg caacccgaat gtgccggtgc atggcttcta
    13141 cgccgccggc gaatgtgcct gtgcctcggt gcatggcgcc aatcgcctcg gcaccaactc
    13201 cctgctcgat ctgctggtgt tcggcaagtc cgccggcgag accgtggtgg cggatttcaa
    13261 ggccggtcag ttgcccctca agccgctgcc tgacaacgcg gccgacgtct ctctggcgcg
    13321 gattgcccgc ctggaaggtc agacgggcgg cgaggaagtc gccgatgtcc ggattgccat
    13381 gcagcgcacc atgcagaagc atgccggcgt gttccgcttt gccgacatgc tgaaggaagg
    13441 tgccgcgaag atcctcgaag tggccgagcg cgccaaggtc acccagatca aggacaagtc
    13501 ccaggtctgg aacactgcac ggatcgaagc cctggagctc gacaacctca tcgaggtggc
    13561 caaggcgacc atggtgtccg cagaggcccg caaggaatcc cgtggcgccc acgtgcgcga
    13621 cgacgcgatc gactgcgccg agacccccaa cggccgtgac gacaagaact ggctcaagca
    13681 cactttgtgg tacagcgaag gcaaccgact cgactacaag ccggtcaacc tgcagccgat
    13741 gtcccccgaa gtcgagccca tcgcgctcgc caagcgtacc tactgagcgc cccggagaga
    13801 aacactatga acaagcgaac cgtacagttc aagatctatc gttacgatcc ggacaaggac
    13861 aaccagccct acatgcagga catctccgtc gaactcgaag agtccgacaa gaagctgctg
    13921 gacgcgctcg tccgactgaa ggccaaggat gattccctgt cattccgtcg atcctgtcgc
    13981 gaaggggtgt gtggctccga cgccatgaac atcaacggta agaacggcct ggcctgtctg
    14041 accggcatcg atgatctggc gcagcccatc accatccgtc ccttgcccgg cctgccggtc
    14101 atccgtgacg tgatcgtgga catgacccag ttcttcaagc agtaccactc gatcaagccg
    14161 tacctgatca acaacgagcc ggcacccgag cgtgagcgcc tgcagacccc ggaagaccgg
    14221 gaggagctca acggcctcta cgagtgcatc ctgtgcgcct gctgttccac gagctgcccg
    14281 tccttctggt ggaatccgga caagttcgtc ggtccggcgg gtctgcttgc ggcgtaccgc
    14341 ttcctggcgg atacccgcga ccaggccacc agcgagcgtc tcgataacct cgaggatccg
    14401 tatcgtctgt tccgttgcca ctcgatcatg aactgcgtcg atgtctgccc gaagggcttg
    14461 aacccgacca aggcgattgg caagatcaag gacatgatgc tgcgccgcgc cgtatgacgc
    14521 tcaaccgggg gcgcgtgcgc tggcagtgca gacgggctct gctggagctc gatctggtgt
    14581 tcacgcgctt tctcgatacg catttcgatc atctcaccga tgatcaagtg gcggatctgg
    14641 agagtctgct ggttgtcgag gaccatgacc tgtgggctat ggtcaacggc agcaagccgg
    14701 tctccgacga ccgctggaag gaaatggtga aactgctgcg tgctggatga cgcagcagtt
    14761 tttccgctag ggagctggag tttcaacagg gataaaggga ccaaaagatg accacgcaac
    14821 gcactgctgc actgaatgta gatggtaagt ccgtagagtt tccgatcatg tcgggcactc
    14881 atggcaatga tgtgatcgat atccgcaccc tcggcgggaa gaccgggctg ttcacgtacg
    14941 attccggctt cctctccacc gccagctgcc agtccaagat cacctatatc gatggtgaca
    15001 agggcgaact gctgtatcgg ggctacccga tcgagcaact ggccgagcag tgcaacttcc
    15061 tggaagtcac gtacctgctg aaaaacggtg aactgcccaa caccacccag aaggtcgact
    15121 tcgagaacac catcaagcgt cacacgatgg tgcatgagca actcgccaag ttctactccg
    15181 gcttccgccg cgacgcccac ccgatggccg tcatgaccgg tgtcgtcggc gccctgtccg
    15241 cgttctacca cgacgccatg gacttcgccg atgccgagca ccgcaacgtc agcttcaacc
    15301 gtctgatcgc caagatgccg accatcgtgg ccatggcata caagtacaac atgggcatgc
    15361 cgttcatgta tccggacaac gaactggatt acacggcgaa cttcatgcgc atgatgttcg
    15421 gcaacccctg cgagaagtac gtgccgaacc cgacgctcgt tcgcgccctc gacatcatct
    15481 tcacgctgca cgccgaccac gagcagaacg cttccacctc caccgtccgt ctggccggtt
    15541 cgtcgggtgc caatccgttc gcctgtatcg ctgccggtat cgcctgtctg tggggtccgg
    15601 cccacggcgg tgccaacgaa gcctgcctca agatgctcga ggagatcggt gacgtgtccc
    15661 gtgtcggcga atacatcaac cgcgccaagg acaagaacga caccttcaag ctgatgggct
    15721 tcggccaccg cgtgtacaag aacttcgatc cgcgcgccaa gctgatgcgc aaggtttgct
    15781 acgacgtgct gaccgaactg ggcctcgaga acgatcgcct cttcaagctc gccatggagc
    15841 tcgagaagat cgccctcgaa gatcagtact ttgtcgagaa gaagctctac ccgaacgtgg
    15901 acttctactc tggcatcgtc cagaaggcgc tgggcatccc gaccgagatg ttcacctgca
    15961 tcttcgcgct ggcccgcacc gtcggctgga tgacgcagtg ggaagaaatg atcaccgatc
    16021 cggaatacaa gatcggtcgt ccgcgtcagc tgtacatcgg cgctgcgcgc cgcgacgttg
    16081 tgcctctggc ccagcgcggc taagcagccg gagggggagg gggaaaccgg cgaggcaatg
    16141 cggggtggtc gcatcatcag gccatcccgc tttgtttttc cgcgaacagt tgcagacaag
    16201 ggattctccc caccggaata tacccaagcc aataaataag actcgtacaa gtcaggtggt
    16261 cgcccatgat gaaacagctt ttcaacacgt cccagctctt cggcagcaac gcgccgttca
    16321 tcgaagagct ctatgaagcc tatctagaca atcccgattc ggtctccgac gagtggcgca
    16381 gctatttcga tacgctgcag aacacccccg gtaacggccg cgatgtcgcc cattacccga
    16441 tcatcaacgc cttcgccgat atggccaagc gcggcccggt gcgtaccgtg gcagccggtg
    16501 gcggtgacca gaagcaggtc agcgtcctgc aactgatcaa cgcccaccgc ttcctgggca
    16561 cccgctgggc gcagctcgat ccgctcaagc gcaccgagcg tcccgagatc cacgaactcg
    16621 agctgtccca ctacggcttc accgagggtg atctcaacca gacctacaac accggttcct
    16681 tccacggtct gcctggcgac cacgccaccc tgcgcgagat cctggatgcc gtgcgtcaga
    16741 cctactgcgg ctccatcggc gccgagtaca tgtacatcac ggacattgcc gagaagcgct
    16801 ggatccaggc ccgtctcgaa ccgactcgcg gcaagcccgc cttcccggcg gaacagcagc
    16861 gccgcatcct cgaacgcctg accgctgcag agacgatgga gcgctacctg cacaccaagt
    16921 acgtgggcca gaagcgcttc tccctcgaag gtggtgaaag caccatcgta gcgatggacg
    16981 agctgatccg cgtggccggc tcccagggcg tggccgagat cgtcatcggc atggctcacc
    17041 gtggccgtct caatgtgctg gtgaacaccc tcggcaagtc gccgaagatg ttgttctccg
    17101 aattcgaagg caaggctgcg tccgatctct ccgcgggcga cgtgaagtac cacatgggct
    17161 tctccagcga tgtgatgacg tccggcggtc cggtgcacct gaccctggcc ttcaacccgt
    17221 cccacctgga aatcgtcaac ccggtggtcg aaggctccgt ctatgcccgc cagacccggc
    17281 gcggtgagaa cggcaaggcc gaggtgatcc ccgtcatcat ccacggtgac gccgccgtcg
    17341 ccggccaggg tgtcaaccag gaaatgctca acttcgcgca gacccgcggc tatggcacgg
    17401 gcggcacgat ccacgtcgtg gtgaataacc agatcggttt caccacctcc gatccgcgcg
    17461 actaccgctc gtccctgtac tgcacggaca tcttcaagat ggccgaggcg ccgatcttcc
    17521 acgtcaatgg cgacgatccg gaggccgtgg ccttcgtgac gggtctggct gtcgaatacc
    17581 gccagacctt caagaaggac gtggtcatcg acatcgtctg ctatcgcaag ctcggccaca
    17641 acgaagccga cgagccgatg gtgacccagc cgctgatgta caagaagatc gccaagcacc
    17701 ccggcacccg caagctgtac gcggaaaagc tggtgcagct gggtgtctgc aaggccgagg
    17761 atcccgatca gttcatcaag gattaccgcg ccgcgctgga caagggcgaa ctcgtcacca
    17821 accccgtgct gtccgacttc aagcgccagt tctcccagga ctggagcccg tacgttaaca
    17881 agaagtacac cgacaagtgc gattcccgag tgccgatggc cgagctgaag cgtctgtcca
    17941 aggcgctcac caccacgccc gaaggtttcg tgctgcatcc ccgcgtccag aagatcatcg
    18001 acgatcgcaa ggcaatgggt gaaggcaagc tggccttaga ctggggcatg ggtgaaaacc
    18061 tggcctacgc cagcctgctg gtgcagggct atggcgtccg gctctccggt gaggacgtag
    18121 gccgcggtac cttcttccac cgtcatgcgg ccctgcacga tcagaaccgc gaccagtggc
    18181 acgatggcac cttccatccg ctcaagaaca tccgcgaagg tcagggcccg ttctactgct
    18241 acgactccgt gctgtccgaa gaggccgtgc tggccttcga atacggctac gcctccgccg
    18301 agcccaacca gctcgtcatc tgggaagcgc agtttggcga cttcgccaac ggtgcccagg
    18361 tggtgatgga ccagttcatc agctctggcg aagccaaatg gggtcgccag tgtggtctgg
    18421 tcatgatgct gccccacggc tatgaaggtc agggtcccga gcactcctcc gcccgcatcg
    18481 agcgctacat gcagctgtgc gccgagatga acatggaagt gtgcatcccg tccggcccgt
    18541 cgcagatctt ccacctgctg cgccgtcagg ccctgcgtaa gctgcgcaag ccgctggtga
    18601 tcatcacgcc caagtccctg ctgcgtcaca aggatgccgt gtcgtccctg gacgatctct
    18661 ccaagggcac cttccagacc gtcatcggcg aagtggacga tctggatgcc aagaaggtca
    18721 agcgggtgct cctgtgctcc ggcaagatct actacgaact gctggcccac cgccgtgcca
    18781 acaacatcga cgacatcgcc atcgtgcgca tggaacagct ctatccgttc ccggccgatg
    18841 ccttctcggc cgaggtcaac aagtacccca acgcgaagga agtcgtgtgg gtgcaggaag
    18901 agccgcgtaa ccagggcgtc tggtactggc tggcgtcccg ccatcacctg gacaccaacc
    18961 tgagcgacga ccacaagttc ctgctggtgg cgcgcccggc agccgcttcg ccggcggtgg
    19021 ggtactacgc caagcacaat gcgcaacaaa aggctgttat cgaaaatgcg ttcggcgaaa
    19081 tcaatgcctg atgctcatgt ccgtgccgaa ttgaacaaga gggagaaaac atgctgatcg
    19141 aagtcaaagt gccgcaactg tccgagtccg tctccgaagc gaccctggtg agctggcaca
    19201 agaaggaagg cgacactgtc agtcgcgacg agaacctgat cgatatcgaa accgacaagg
    19261 tcgtgctgga aaccccggcc ccggccgacg gcgtgctggt caagatcgtc aagaacggtg
    19321 gcgattccgt cacctccggc gaagtgattg ccaccatcga cactgaagcc aaggccgctg
    19381 ctgcgccggc cgctgcggct gctccggcac cggctgctgc gcccgctgcg gctgcgccgg
    19441 ccgctgcggt ctccgccgcc agcccctcgg cccgcaagat cctggacgag aagggcatcg
    19501 ctgccgctga cgtggccggt agcggtcgtg gtggtcgtgt gaccaaggaa gacgccgtgg
    19561 ctgcccagcc caaggccgct gccgccgcgc cggccgtggc cgtgccgacc ggcgaccgcg
    19621 tcgaacagcg cgtgccgatg acccgtctgc gcgcccgtat cgccgagcgt ctgatccagt
    19681 ccaagaacga aaacgccatc ctgaccacct tcaacgaagt caacatggcc ccgatcatgg
    19741 ccctgcgcaa gcagtacggg gacaagttcg agaaggctca cggcgtgcgt ctgggcttca
    19801 tgggcttctt cgtcaaggcc gctgtcgccg cgctcaagaa gttcccgatc ctgaacgcct
    19861 ccgtggatgg caacgacatc gtgtaccacg gttacatcga catcggcatc gccgtgggct
    19921 ccccgcgcgg tctggtggtg cccatcctgc gtgatgtgga tcagatgtcc atcgccgaca
    19981 tcgagaagaa gatcgccgaa ttcggccaga aggccaagga cggcaagatc tccatcgaag
    20041 agctcaccgg tggcaccttc tccatctcca acggcggcgt gttcggttcc atgatgtcca
    20101 ccccgatcat caacccgccc cagtccgcga tcctcggcat ccacgccacc aaggaccgcg
    20161 ccgtggtcga gaatggtcag gtcgtcgtgc gtccgatcaa ctacctcgcc atgtcctatg
    20221 accaccggat catcgacggc cgtgaagccg tgctgggtct ggtgaccatg aaggaagcgc
    20281 tggaagatcc ggcccgtctg atcctcgacg tctgatcttc cgatccgggg cacgcgggcg
    20341 acagcgatcc tgtcgccccg tgccttgttt ccctccgcac aaggaactct cacatggcga
    20401 agcaatttga cgttctggtc atcggcggtg gtcccggtgg ctacgtggcc gcgatccgcg
    20461 ccgcgcaact cggtttcagc acggcctgcg ccgaatccaa tccctatgcc gacccgaagg
    20521 gcgaaccccg tctgggcggc acctgcctga acgtgggctg catcccctcc aaggcgctgc
    20581 tgcacacgtc ccacctgttc gaagaagccg gtcatgcctt cgaggagcag ggcatctccg
    20641 ttggcaagcc caagatcgac gtgcccacca tgatcgggcg caagtccaag atcgtcgagc
    20701 agctcaccca gggcatcaag ggtctgttca agaagaacaa ggtcaccttc ctggccggtc
    20761 acggttcctt cgtggccccc atcgacggtg gctggcaggt caaggttggt gaagaggtcg
    20821 tcgaggccaa gcaggtcatc gtcgcgaccg gctccacggc ccgccacctg ccgggcattc
    20881 cggtggacaa caagctggtg tgcgacaaca tcggcgcgct cgacatcgac gccgtgccca
    20941 agcgcctcgg cctgatcggc tccggcgtga tcggcctgga aatgggttcc gtgtggaacc
    21001 gcctgggcgc cgaagtcacc atcctggaag ccatggactc cttcctggcc gccgctgacc
    21061 aggatgtggc caaggaagcg ctcaagctct tcaccaagca gggcctggac atcaagctgt
    21121 ccgtcaaggt cggcgccgtc acggtcggca agaagtccgt caccgtcgag tacgagacca
    21181 aggatggcgc ccagaaggcc gagttcgacc gactgatcgt ctccgtgggt cgcgtgccca
    21241 acaccgccgg cctcaacgcc gaagcggtgg ggctcaagat cgacgagcgc ggcttcgtgg
    21301 acgtggatgg ccacaaccgc accaacctgc cgggcgtctg ggccatcggc gatgtggtgc
    21361 gtggccccat gttggcccac aaggcgatgg aagagggcgt ggccgtggcc gagacgatgg
    21421 ccggtcagaa gggtcacacc aacttcgaca ccatcccctg ggtcatctac acctcgcccg
    21481 agatcgcctg ggtcggcaag accgagcagc agctcaaggc cgaaggcgtc gcctacaagg
    21541 ttggcaagat ccccttcctg gccaacggcc gggccctggg ggccggtgcg ccggcgggct
    21601 tcgtcaagat gctggcggat gccaacaccg accgcgtgct gggtgtgcac atcatcggcg
    21661 ccaatgcctc cgagctcatc ggtgaggccg tggtgacgat ggagttcgct ggcgcgtccg
    21721 aagacctggc ccgcatctgc catgcccacc cgaccctgtc ggaagtcgtg cacgaagcgg
    21781 ccctggcctg cgacaagcgt ccgctgcact tctgatgtga caccgggccg gcccgtgccg
    21841 gcccccgcac caccgggggt gggaggtagg cgcctacctc ccgcccccgt tccattgaat
    21901 cacccggaaa tacggtctcg accatgccgc accgcatcct caacgtttcc gaaaagggca
    21961 tgctggacgc ctatgaaggc ctcctccgcg cccggggcta caagtccgat ccggcccagc
    22021 gtgccgctgc ccagcgcctg cagggcatgt actccgaact ggtggccttc aaggccgcac
    22081 ggcgcggggc catccgcaag ctgctgtccc gtcccaagat gccccgcagc gtctatttct
    22141 ggggtggcgt ggggcgcggc aagagctttc tgatggactg cttcttcgat tcggtgccct
    22201 accagcgcaa gcgccgggtg cacttccatg ccttcatgca ggaagtgcag aacgatctga
    22261 agaacctcaa ccacgagccc gatcccctgc agaaggtggc cgaccgcatc gcgcgtcaga
    22321 cccgcctgct gtgcttcgac gagttccacg tctccgacat cgccgacgcc atgatcctcg
    22381 gccgtctgct cgacgccctg ttccagcgtg gcacgatctt cgtgatgacc tccaactacc
    22441 cgccagacgg tctgtatccc aatggtctgc agcgcatcaa cttcctgccc accatcgaca
    22501 tgatcaagcg gcgtttcgac gtggtggagg tggatcacgg caccgactac cgcctgcgca
    22561 gtctggagaa gatggaagcc ttcctggtgc ccggcgacga tgtcgcggac aagcgcctgg
    22621 cgtccgattt cgcccagatc gccggcacgc cgggggaggg tggcgagatc gaggtgctcg
    22681 agcgcacgct caaggtcgta cgtcaggcgc ccggcgtcat ctggttcgac tttgccaccc
    22741 tgtgcggggg cgcccgttcc cagaacgact acctggagat cgcccgcgag catcacaccc
    22801 tgatcctctc ccgcgtgccg aagatgaacg ccggcatggc ctccgaggct cggcgcttca
    22861 cctggctcat cgacgtgctc tatgaccacc gggtcaagct gctggtcagc gccgaatgcg
    22921 aggcctacga cctctatcgc gagggccatc aggccaacga gttccagcgc accgtcagcc
    22981 gcctgatgga gatgcgctcg cgggaatacc tggccgaggc ccaccgggtc gactgagcga
    23041 tccatggggc gtgggcgttt caccgtcttg cggggcgcga tggcggccat cgtcgggctc
    23101 ggcgtgcccg ccgtcccggc ccaggcgccc gtcggacgcc tggagatcat ccctctgcgg
    23161 catcgccccg ccgatcagat cgtggcggcg atccggcccc tcgtggcgcc cggcggcgcc
    23221 ctcagcgcga tgggcgacaa gctcgtcctg cgcacctcgc cggccaatct ggccgagctg
    23281 cgccgcgcca tcgaagccct cgacacgccg atccgccggc tggtgatttc cgtgcgtcag
    23341 ggcgatgtgc gggactttgc gatggcggac ggtggcgtgg ggggcatctt tcgtcccggc
    23401 gacagccgtg tcgtcatccg gggcgacgcc cgtcagggca gcgcccgcga gcagatcgac
    23461 cagcaggtcg aggtgatcga gggcggtcag gccctgatcc gggtcggcca gtccgtgccc
    23521 ctgcggatgc gccagggcta tgccacgccc gacggtggct gggtgacggc cgacagcatc
    23581 gcgcttgtcg acgtgggcag cggtttctac gccgcgcccc agctcgttgg cgaacaggtg
    23641 cgcatcgaga tccgtcccga gcaggctcgc ctggacgctg gcgggcaggt cgaatccgcg
    23701 cgcctcgcga ccaccgtgag cggccctctg ggggcctgga ttcccctcgg tggcagcacc
    23761 acggaatcca ctcgcaacgc ggccggtctg tctggcgcgg cgcagggccg cgagacgctc
    23821 agccggcagg tgtggctgcg ggtagacgcc ctcgactgag cccatggata tcgacaccgc
    23881 cttcgccgcc gacggccccc tcgccgccgc cattcccggc tttgcgccgc gcccccagca
    23941 gctcgagatg gcccgccaga tcgccgacac cctgcgcgcc aaccgggtgc tggtggccga
    24001 ggccggcacc ggcaccggca agaccttcgc gtacctcgtg ccggcccttc tcaacggcgg
    24061 caaggtcatc ctgtccaccg gcaccaagac cctgcaggac cagctcttca accgggatct
    24121 ccccaccgtg cggggcgccc tcaaggtgcc cgttgccgtc gccctcctca agggccgcgc
    24181 caactacgtg tgtcactatc acctggcgcg caacctggtg gacggacgct tccagagccc
    24241 ccaggacgcc gtccacctgc gcgccatcgg ccgcttcctg gaaatcaccc ggagcggcga
    24301 caaggccgac tgcccggagg tgccggaaga cgcgcccgcg tggatgtacg ccacctcttc
    24361 gcgggacaac tgtctgggtc aggactgtcc caacatcaag gaatgtttcg tcgccgccgc
    24421 ccgtcgggcg gccatggagg ccgacgtggt ggtggtcaat caccacctct tcttcgccga
    24481 cgtgatgctg cgcgacgaag gcatgggcga actgctgccg tcctgcaacg cggtgatctt
    24541 cgacgaagcc caccaactgc ccgagaccgc cagcctgttc ttcggcgaga gcgtgtccac
    24601 tgcccaggtg ctggagctgg cccgcgatac ccgctccgag accgtcgctg ccgcgcggga
    24661 ctgcgtggat ctcatcgaag ccacccgcag cctcgaaaag gctgcccgcg acctgcgcct
    24721 cgcggtgagc gccgagaacg cccgcttcgg ctttgcccag ctcgccgaca accccggctt
    24781 ccacgaagcg gtggcgcatc tcgacgacga actgggtgcc ttccgcattc tcgtcgaatc
    24841 ccaggccgaa cgctccgaag ggctggccgc gtgcctgcgc cgcacgcagg agatccagga
    24901 acgcctcacc cgctggcgcg cgccggagaa caccgactgg gtgcgctggg tcgaggtctt
    24961 cagccagacc ctggccctca acgccacgcc cctgtccatc gcctccatct tcaagcgcca
    25021 gttggacggc cacccacggg cctggatctt cacctccgcg accctcgccg tcggccccga
    25081 ttttggccac tactgccgcg aactgggcct gcactggctg gaaccacccc ccgataccgc
    25141 cctgtggggc agtcccttcg actacccgca gcaggccatc ctctatgcgc cccaggacat
    25201 gccggacccc aacagcccgg tctatccgga ggccgtcgcg cgggtcgcct atcccctgat
    25261 ccgcgccgcc caggggcgca ccttcgtgct gtgtaccgcc ctcaaggcca tgcgccgtat
    25321 ccacgaggtg attgccgaca agctcgccgc cgagcagctc gatctgcccc tgctgctcca
    25381 gggcgaaggc tcccgctcgg agctccttgc ccgcttccgg cgcctgggca atgcgatcct
    25441 ggtggccagc cagagcttct gggaaggcgt ggacgtgccg ggcgacgccc tctcggtggt
    25501 ggtcatcgac aagctgccct ttgcgccgcc cgacgacccg gtcctcgccg cgcgcatcga
    25561 acacctccag aagagcggcc gtaatccctt catggagtac cagctgccgc gcgccgtcat
    25621 caacgtcaag cagggcgccg gccgcctcat ccgcaccgag cgcgaccgag gcgtcctcgc
    25681 catctgcgac ccgcgcatgc tcagcaagcc ctacggccgg cgtgtctggc agagcctgcc
    25741 gccgatgcgg cgcagcaagg tggaggccga agtggtggcg tttctagaag acctgccgcc
    25801 gcctgccgcg ggtgggtgct gagcgtcgct accgcttgct acaatccgcc acctgtaccg
    25861 atccgccaat cgcccgaacc cgtttcccga ccccaccgtt gctttcgtga ccacccgtct
    25921 gcgctccgcc gaccctgaac ccgccgaccg ggcccactct acgggcacgg cgggctgtgg
    25981 cggacgggcc gaaaccctga acgccacctg ccagtgcatc accctcgaca ggccggccct
    26041 gcgcgaggcc ctggcgcggg atccgcggga cggggctctg accgcgctcc tcgccgaatc
    26101 ccgtccgcac ctgttctccg acgccatggc cttcgtcgcc caggcccacc tggagcacat
    26161 ggcagaggtg gtcgccgcca tcgaacgggt cgttgcgcag cccgcctggc aggctgccgt
    26221 gctggccgat gcgcccgtcg cggcgcggcg tccaggggcg gcgcagggcg tcttccttgg
    26281 ttacgacttt cacctcgggc ccgacggacc gcggctcatc gagatcaaca ccaatgccgg
    26341 gggcggactg ctcagcgctg tgctcggccg tgcccagcgc gcctgctgtg cggaagtcgg
    26401 cagcctgatg cgcgccgccg tgccgacggg gaccgacgat gcggaaaccg ccttcctcgc
    26461 catgttccgc gccgaatggc aggcggagcg gggcgacgcg cccctgcggc gcatcgccat
    26521 cgtggatgat cggcctggcg accagtatct gctgcccgaa tttttgctct tccagcgcct
    26581 cttcgagcgc cacggcatcg ccgcggtggt ctgcgatccg gccgagctgc gctgcgagcg
    26641 gggctgcctg tggcacgacg gtcagcgggt ggacatggtc tacaaccgcc tcaccgactt
    26701 cgcccttgac gaacctgccc acgccgccct gcgtgccgcg tggaacacgg atgccgcggt
    26761 gatcacaccc catccccgcg cccacgccct ctacgcggac aagcgcaatc tggtccgcct
    26821 gtccgacccg gacctgctgc gcgggctggg cgtcgatgcc gagacgcagg ccgtcctggg
    26881 cgccggcgtg ccgcgcaccg aggcggtcac gccagaccgg gcggaggccc tgtgggcggc
    26941 gcggcggggc ctcttcttca agcccgcgtc gggcttcggc agccgcggcg cctaccgggg
    27001 cgacaagctc acccggcggg tctttgccga gatcctggct ggtgattaca tcgcccaggc
    27061 cctggtgccg ccctccgagc ggcgcgtgac cgtggatggc gcggcggtgg atctgaagat
    27121 ggacctgcgc aactacgtgt atgggggcca ggtccagctc gtgaccgcgc gcctgtatcg
    27181 gggccagacc accaatttcc gcacccccgg cggcggcttc gcgccggtgc tggcggtgcc
    27241 ctgcgacgat gcccgaggat gaggggatga aggtgttcgg attcgccggc tggtccggct
    27301 cgggcaagac gaccctgatg gagcgggtga tcccgctgct caccgggcgg ggcctgaagg
    27361 tgtcggtgat caagcacacc caccaccgct tcgacctgga ccagccgggc aaggattcct
    27421 ggcgtcaccg ggaggcgggc gccgccgagg tgctgctgat ctcggaaagg cgctgggccc
    27481 tgatgcatga gctgagggac gagccggaac ccgatctggc ggcccagctg gcgcgcatgt
    27541 cgccctgcga cctggtgctg gtggagggct tcaagcacct gggcctcccc aagctcgaga
    27601 tccatcgtcc ggcccacggg cgtccgctgc tccatctgtc cgatccggcc atcgtggccg
    27661 tggcgaccga cggggcactt gcgacggacc tgccttgtct caatctcaac gatccggccg
    27721 cggtggccgg cttcatcgtg gatcacctgg aactctgaca tgagctcaac cctgcgttcc
    27781 tacgaggacg tgcgcgacgc cctgatcgcc ctggcccgac ccgtcgtcga gaccgaggtc
    27841 ctgcccaccg ccgaggccct cggccgcgtg ctggccgatg ccgtggtgtc gccgctggag
    27901 gtgccgcccc tcgacaattc ctatatggac ggctacgccg tgcggatcgc cgatctgccc
    27961 gccccgggaa cccgcctgcc ggtgagtcag cgcatcccgg ccggcagcgt gggcagccgc
    28021 ttgcagcccg gcacggcggc ccgcattttc accggcgcgc ccattccggc cggcgccgat
    28081 gcggtggtga tgcaggagtt ctgctcgccg gacggagacg gcgtggtgat cgatcacctg
    28141 ccgcgacccg gcgaatggat ccgtcgggcc ggcgaggaca tcgcccgcgg tcagaccatc
    28201 ctccacgccg gcgaccggct gacgccccag cgcctcggtc tggcggcctc cgtgggcgcg
    28261 gcgcatctga cggtccggcg gcgtctgcgg gtggccctgt tctccaccgg tgacgagctg
    28321 gtcatgcccg gcgaggcctt gccgccgggc gccatctaca actccaaccg cttcacgctg
    28381 cggggcctgc tggaaggact gggctgccaa gtggacgacc ggggcatcgt gcccgaccgg
    28441 ctcgacgcga cccgcgccgc cctgcgcggt gcggcggagg tgagcgacct gatcctcacc
    28501 agcggtggcg tctccaccgg cgaggaggac cacatcaagc cggcggtgca ggccgagggg
    28561 cgcctggact cctggcaggt cgccatgaag ccgggcaagc cggtggcctt cgggcatgtg
    28621 aacggggccg ccgggacggc gggcgccgcc ttcatcggcc tgcccggcaa tccggtctcc
    28681 agctttgtca ccttcctggt gctggtccgg cctttcatcc tgcgtgcgca gggggtgcgc
    28741 gaggtgctgc cccgcgcgct gcccctgacg gccgccttcg acctgccccg gccggaccgg
    28801 cgacgggagt tcctccgcgc ccgtctcggt gccgacgggc gcgtcgagat ccatcccaac
    28861 cagggaccgg cggtgttcac gtcgacggtg tgggccgagg gcctggtcga caatcctccc
    28921 ggtcacgtcg tccaggcggg cgaccgggtc cattacattc cctattccga actgctgtca
    28981 tgagcgtcaa agtcctgtat ttcgccagcc tgcgggaaac cctcggctac tcccacgaaa
    29041 ccctgaccct gcccacgcag atcgccactc tgggcgacct gcgcacccac ctggcccgcc
    29101 gcggcggtgt gtgggaggcg ctgggggagg gccgcaacgt gcgcgctgcc atcaaccagg
    29161 agatggccgg ccccgagcgc ccggtggccg accgggacga gattgccttt tttccgccgg
    29221 tgaccggagg ctaggatgag cacaacggga tttcgcatcc aggtgcagga ggccgacttc
    29281 gacaccggcg ccgagatcgc cgcgctgtcg gcgggtcgcg acgacgtggg tgcggtggcc
    29341 agcttcgtcg gcctggtgcg ggccgacaag tccgcctgcc agaccggcca cgtctcggcc
    29401 atgaccctgg aacactaccc cggcatgacc gagaaggccc tcgaagccat cgtggacgaa
    29461 gccgccgggc gctggtccct gcagggcgtg cgggtcatcc accgcttcgg ccggctgctg
    29521 cccggcgagc gcatcgtctt cgtcggggtg gcgagcagcc accggggcga cgccttcgct
    29581 gcctgcgaat tcatcatgga ctacctcaag acccgcgccc cgttctggaa aaaggaagac
    29641 acccccgcgg gcggccgctg ggtggacgcc cgcgaagccg acgacagcgc tgcggcgcgc
    29701 tgggcgcagc ccggtggtga ttgatgcgct tccccggtcc gctgcggcgg tcgcgggcgt
    29761 ccccctgcgt ccggtcgtcc gacccgcgcc ggtcccggcg gacggcgtcc gttccgcctc
    29821 caacgcctgc atgggcagcg tgactgctcc gccgctttcc gcccgcagct aggccatgtg
    29881 gatcgtccgg atcgccctgc agcggcctta caccttcatc gtgatggccc tgctgatcgt
    29941 tctggccacg ccgctggtgc tgctgcgcat cgccacggac gtgctgcccg agatcaacat
    30001 cccggtcatc agcgtgatct ggacctacac cggcatgtcg gcccaggaaa tggcccagcg
    30061 cgtcaccggc cccaacgagc gcctgctggc gaccacggtc aacgacgtgg agcacttcga
    30121 gtcccagtcc tacgccggca tcgccatcat caagctgttc ctccagccca aggccagcgt
    30181 cgaggcggcc ctcgcccaga tcgcgtccac cgagggcggc gccctgcgcc agctgccgcc
    30241 gggcatcacg ccgcccctcg tcattcgcta caccgcatcg gccattccgg tggtgcagct
    30301 gggcatctcc agcgcgtccc tgccggagca ggtgatgttc gacaccgcgg tgaacaccct
    30361 gcggccccgc ctggtgtcca ttccgggcgt cgcgctaccg tttccgtatg ggggcaagag
    30421 ccgtgtggtg tccgtggacc tggatctgcc ggcgctgcag gccaaggggc tcgcgccgct
    30481 ggacgtggtc aatgccatca acgcccagaa cctgatcctg cccggcggca cggccaagat
    30541 cggtgccacc gagttcgcgg tgaagatgaa cggctcgacg cgcagcgtgg aggagctcaa
    30601 cgacctgccg gtgcgcctgg gcagcggcgc ggcggtctat ctgcgggacg tggccgtggt
    30661 gcgggacggc ttttcgcccc agaccaacat cgtgcgccag gacggcaagc gcggcgtgat
    30721 ccagtccgtg ctcaaaaacg gcggcgcctc cacgcttcag atcgtggaca acctgcgcac
    30781 cgccctggcc gacgccctgc ccagcctgcc cgaggacatc cgcgtcacgc ccctgttcga
    30841 ccagtccgtg ttcgtccgcg cggccatcgg cggggtggtg cacgaaggcc tcatcgccgc
    30901 ctgcctcacg gccgcgatga tcctgctctt cctcggcaac tggcgcacca cctgcatcgt
    30961 cgccatctcg attcccctgt cggtgctggc ctccatcctc accctctacg ccctcggcga
    31021 gaccatcaac atcatgaccc tcggcgggtt ggcgctggcg gtgggcattc tggtggatga
    31081 cgctaccgtc accatcgaga acatcgagcg ctacctgcac ctgggccacg acctggagac
    31141 tgccatcctc gaaggcgccg gcgagatcgc cgtgccggcg ctggtgtcga ccctgtgcat
    31201 ctgcatcgtc ttcgtgccca tgttcttcct caccggcgtg gcccgttacc tctttgtgcc
    31261 cctggccgag gcggtgatct tcgccatgct ggcctcctac ctgctgtccc gcaccctggt
    31321 cccgaccctc gccctgctgt tcatgaaggg ccagcacggt ggcggggcgg gcggctggct
    31381 gcagcccctc tacacccgct tcgacgccgc cttcgagcgc ctgcgggggg gctacatcgt
    31441 gctcctgagc gtgctgctgc cgcgccggcg ccgcttcatc gccgccttcc tggcggtgtg
    31501 cgccctgtcc atgggcctgt acgccgtgct cggccgcgac ttcttccccg cggtggatgc
    31561 gggcatcgtc cgcctccact tccgcgcccc cagcggcacc cgcatcgagg agacggcgcg
    31621 cctggcggac cgcatcgagg cggccctgcg cgaactcatc ccggccgacc agatcaccac
    31681 cctggtcgac aacctgggcc tgcccaacag cggcatcaac ctctcctaca gcaatgccgg
    31741 caccatcggc accctggacg gcgagatcca ggtcgccctg gcgcccgggc atgggtcgtc
    31801 cgccaacctc gtcgagcgcc tgcgggacgc gctgcccccg cgctttccgg gcatcgagtt
    31861 cttcttccag cctgccgacg tggtcaccca gatcctcaac ttcggcctgc cggccgcgat
    31921 cgacgtgcag atcagcggcc tgcgcatggc cgagaactac cgcgtcgccg cccagctcga
    31981 aaaagccgtc cggcgtattc ccggcgcggt cgacgtccat atccaccagc gcttcgacct
    32041 gcccacggtc aacctggcca tggaccgcaa ccgcctgcag caactcggcc tgtccgccgc
    32101 caacgtggcc cagaacgtcc tgatccctct ctccggcagc ttccagaccg ccccgagctt
    32161 ctggctcaat ccccggaacg gtgtgtccta caacatcacc gtgcagtcgc cccagtaccg
    32221 catcgactcc ctcgacagcc tgctccagat ccccgtggcc ggcagcgcgg gcacgcccgg
    32281 ccagctcctg tccaacctcg tgcaggtggg ttccggccgc gagccggcca tcgtctcccg
    32341 ctacaacatc gaaccggcgg tggatctcta cgtcagcgtg cacggccgcg acctgggggc
    32401 cgtggtggcc gacgtggagc gcgaggtgga ggccgtccgc gggctgctgc ccaaaggctc
    32461 gcgcatcagc ctgcagggcc aggcccgcac catgaacagc tccttcatcg gcctgggcat
    32521 cggtgtggtg atggccgtcg tgctggtcta cctgctcatc gtcgtgaatt tccagtcctg
    32581 ggtggacgcc ttcatcatca tcaccgcgct gccggccgcc ctggcgggca tcgcctggat
    32641 gctcttcctc accggcacgc ccctcagtgt gccagccctc accggcgcga tcatgaccgt
    32701 cggtgtcgcc acggccaaca gcattctggt ggtgtctttc gcccgccagc gtctgctggc
    32761 cggcgcgccg cccctgtccg cggcgctgga ggcgggcgcc acccgcctgc gtccggtgtt
    32821 gatgacggcc cttgccatga tcatgggcat gctgcccatg gccatcggcc tcggcgaagg
    32881 gggcgagcag aacgccccgc tgggccgcgc cgtgatcggc ggcctgctgt gggcgaccct
    32941 gtccacgctg gtgttcgtgc ccctcgtgtt cgccgccgtg catcggggct ttgcccgtcg
    33001 cacggcgcgt cgggcggcgc tggccggaag catctaggat gattcgcccc tcacgctcac
    33061 tttgttcctg gccccctgga ggggcgccgg tcgtccgtgg gacggcccgg caggaggtct
    33121 gacatgtccg ccaagcgcca cgccagtctc ggcattcacg gtgaccacgc gtccgc
//
LOCUS       DAOQMM010000030        31701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_380_length_31701_cov_50.868325, whole genome shotgun sequence.
ACCESSION   DAOQMM010000030 DAOQMM010000000
VERSION     DAOQMM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 31701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31701
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_31701_cov_50.868325"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLH85_07200"
     CDS             complement(<1..551)
                     /locus_tag="PLH85_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNB64384.1"
                     /translation="MEEFIGGLWHRFITRAASRSHPEAAVRLSEIERTAGILFRALGG
                     DSGLRVAAAAEVEHGARRSWLARIAHAGEKVAHAARDDETLRLPAEIALFPERRLNRD
                     LYLWLIAQGAALPELPGNWIVANQTASAATLARFPGLAARYRRLVAAVLAERPDPARL
                     PADEAAAEAAIRQALTDPGSVMTL"
     gene            complement(560..1051)
                     /locus_tag="PLH85_07205"
     CDS             complement(560..1051)
                     /locus_tag="PLH85_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64385.1"
                     /translation="MSGLNDFALARALHVLAVLIWIGGVAFVTWVLLPACRDLAEPAA
                     RIDLFERLEHRFAAIARAAVLLAGASGLYLVWRLDLWSRFAQPAEWWWMHAMVAVWTV
                     FALMLFVLEPFVLHKLFRQLAQADPDATFARIRRLHGVLLAASLVTVLGAVAGSHGGW
                     IFG"
     gene            complement(1048..2004)
                     /locus_tag="PLH85_07210"
     CDS             complement(1048..2004)
                     /locus_tag="PLH85_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB64386.1"
                     /translation="MQPDGGRQAQRRLAQGAFFVLFVLAPVFDLLRYDLVAGHAWFLG
                     MPWHAGLEDFSAGRIGALEAGLRVLVRVLLPILAAGAVLIGVAWRWGRLYCGWLCPHF
                     SVVETINGLMRRASGKPSVWEPHALPARAPDGSPLPVDPRWWGATVPLAVAFAFVWAV
                     VLLTYLLPPFQVYGHLLAGTPTRNEALFIGAGTVLLSAEFLFARHLFCRFACAVGMFQ
                     SVAWMGNRDAMVVGFERARAAQCVGCDAACEHVCPMRLKPRAIKRAMFTCTQCAQCVS
                     ACATTQRDHPSGPLLHWVSGAAARHNEAAFRAPRAEEWRKHP"
     gene            complement(2043..2444)
                     /locus_tag="PLH85_07215"
     CDS             complement(2043..2444)
                     /locus_tag="PLH85_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64387.1"
                     /translation="MLPVDPADADVAGRELAVAAEALYLANLMLIPGLGFIVLAVLWS
                     LRHRDAPPLARSHLRQTMAASLWAVALLIVANGAILLLGGYRAASTWVVLILYFTLVH
                     SSLILCGAVGLSRALAGRSFRFPVVGPREVA"
     gene            complement(2476..3261)
                     /locus_tag="PLH85_07220"
     CDS             complement(2476..3261)
                     /locus_tag="PLH85_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNB64388.1"
                     /translation="MTANLPFYEAAGNEIQVFEQAWKNRLPLLIKGPTGVGKTRFVAH
                     MAARLGLPLFTVACHDDLTAADLVGRHLLGDGQTVWCDGPLTRAVRQGGICYLDEVVE
                     ARRDTTVVIHPLADDRRVLPIERTGETLHAPDAFMLVMSYNPGYQNFLKGLKPSTRQR
                     FVSLRFEFPAAAREEAIVIGETGCDPMLAKRLVSIAGRLRALKDVDLEEVVSTRLLVY
                     AAKLVKVGLDPVEACRSALVESLTDDADVADALLEVVQATFGR"
     gene            complement(3270..3530)
                     /locus_tag="PLH85_07225"
     CDS             complement(3270..3530)
                     /locus_tag="PLH85_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNB64389.1"
                     /translation="MNVRQLNFAWVVLAVATALTWELGEHGAAGPAAMAALLAISFVK
                     GRLVVLDFMGLRGVKFFWRALLLGWLLVVAGLIALAYWIALR"
     gene            complement(3527..4108)
                     /locus_tag="PLH85_07230"
     CDS             complement(3527..4108)
                     /locus_tag="PLH85_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNB64390.1"
                     /translation="MSTSARPAPAAAPPGDLAIWFFILAELLAFGVFFAAYAFARARH
                     PDLFDSMQATLDRRAGAVNTLLLITGSFCVARAVEAAGRDRSATAARWLAGGLVCGAG
                     FLGVKGVEYAAKFGAGINLSTNTFYMFYLSLTFFHFMHVILGLVILAVLWVQTRRGAY
                     GAGRMNGLESGAAYWHMVDLVWIVLFPLVYVMR"
     gene            4277..5215
                     /locus_tag="PLH85_07235"
     CDS             4277..5215
                     /locus_tag="PLH85_07235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB64391.1"
                     /translation="MKILLVDDSRAVAAVMGARLSGAGYEVILAENGKVAVEQFRASA
                     PDLVLMDIEMPVMNGFQAAQAIRELEATGEWAWTPIIFLTASDTPENLVTAIEAGGDD
                     FIAKILPEAVLEAKMKAMARIARLRDQLSAANRKLTEQASQDGLTGLCNRRYMDLRLD
                     SQWAGCCDAREPFGLLMLDVDHFKKYNDHYGHQAGDDCLKAVARAISETVEAANVRGD
                     VHGALAARYGGEEFAVLMPRCTRDTFRALADAICTAIRTLAIPHERNAEWGIVTASVG
                     GAYAAQAEGRVALRFREADANLYRAKDLGRNRAVLD"
     gene            complement(5232..5723)
                     /locus_tag="PLH85_07240"
     CDS             complement(5232..5723)
                     /locus_tag="PLH85_07240"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNB64392.1"
                     /translation="MNGPTVRWEGLRGEAYVMIQVALLVMLVFGPRTVAGLPPWPAEA
                     ALPALIGGIALMAAGGLIAALGALRLGSNLTPLPHPKDGATLVVSGLYARVRHPIYLG
                     LILSAFGWALFHQGWLTLGWAALLFVLLDVKSRREERWLADRFPAYADYRRRTRRLIP
                     WLY"
     gene            complement(5720..6799)
                     /locus_tag="PLH85_07245"
     CDS             complement(5720..6799)
                     /locus_tag="PLH85_07245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB64393.1"
                     /translation="MTLAEAARGRDNNFHLIRFLAALAVLYGHCYPLAGGGAPPLADW
                     LGIDIGSVAVDVFFVTSGFLVTASLRDRPGVRAFVWARVLRIYPALWGVVVLTVAVLG
                     PLLTTRPAAAYWADPGVVDYLIKCASLWGGIAIHLPGVFEANPYPAAVNGSLWTLPYE
                     LRMYAILALLGIVVQRLGRRRQGVLTGAVALIAGVAGGTMLVRHFFHTDAGYFARFAW
                     MFFCGACLQMAAARVPLGWSGLLAGGGVLALASLQRDVFFVAYVLLLGYLVVALAHVP
                     GGVIRRFNRVGDCSYGVYLYAFPVQQTVVALLPGITPVGLLAVSAPLTVALAGLSWIC
                     VERRALAFKGRMDVERGMATEGSMR"
     gene            6983..7474
                     /locus_tag="PLH85_07250"
     CDS             6983..7474
                     /locus_tag="PLH85_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64394.1"
                     /translation="MGAIFAKTRKGQQEIETRSGGLHPRVRRTLIVVDGKRSVDELRE
                     ALRSDDLQHTLGLLEEEGYIELISGARLAEAAPASISAFRLHPPGEDPLRLQQSRNFM
                     LNTLRTFVGAVGTSSLQDRLEAARTHADLRALFDEWYHALVTSRDGRREAEHLRSKLL
                     EIL"
     gene            complement(7486..7821)
                     /locus_tag="PLH85_07255"
     CDS             complement(7486..7821)
                     /locus_tag="PLH85_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNB64395.1"
                     /translation="MAFDEGLAERLREAFAGMTDVGERRMFGGLALMWRGHMCVGIVG
                     ETLMVRVGPDAYAAALARPHARPMDFTGRPLRGYVYVAPDGFAEDADLAAWVEAARLY
                     VAGLPPKGS"
     gene            7925..8227
                     /locus_tag="PLH85_07260"
     CDS             7925..8227
                     /locus_tag="PLH85_07260"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNB64396.1"
                     /translation="MFKKLSALYTLIREDLRILGFALRHPARPVWLLPALVALGLYVL
                     SPIDLLPDLVPVLGWVDDLILVPLVIAWLVSRLPVDLRRAWQDARRTVDAPAVRRA"
     gene            complement(8232..8564)
                     /locus_tag="PLH85_07265"
     CDS             complement(8232..8564)
                     /locus_tag="PLH85_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNB64397.1"
                     /translation="MDWLLLFVAIVAEVIATSALKLADGFRRLWPWGIVVSCYSLALY
                     LLSLTLDVLPVGVVYAVWSGSGVALMTLVGRYFFGQKLDRPALIGIGLIVAGVVVLQL
                     FSAAVVRA"
     gene            complement(8583..8966)
                     /locus_tag="PLH85_07270"
     CDS             complement(8583..8966)
                     /locus_tag="PLH85_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNB64398.1"
                     /translation="MAAPDPTATPAPAPRAGTLQWVLLFVAIVAEVIATSTLKATEGF
                     TRLWPSVVVVVCYETAFILLSLSLKKIPVGIVYAVWSGVGVALITVVAWVFLDQTLDP
                     AGLLGIGLIVAGVVVINAFSETVTE"
     gene            complement(8966..9178)
                     /locus_tag="PLH85_07275"
     CDS             complement(8966..9178)
                     /locus_tag="PLH85_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64399.1"
                     /translation="MRAFVLAGLLATGLAACGFGDAGSSAATAAKLKAQEAQQAEAVK
                     AQVESQLEAARLQAEQRRRDIEAATR"
     gene            complement(9175..10290)
                     /locus_tag="PLH85_07280"
     CDS             complement(9175..10290)
                     /locus_tag="PLH85_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNB64400.1"
                     /translation="MSPPAAVSALFQPVRLGDLDLANRIVMAPLTRCRVTNGEAAPDA
                     TQAQYYAQRAGAGLIVSEGTIVSPQGRGYPFTPGLWSAAQVAGWRLVTDAVHAAGGRI
                     VCQLWHCGRLSLPEFHGGAAPVAPSAIDPQWKMFGSAGLTPTVTPRALTREEIAGIVA
                     DFARAAAHAREAGFDGVEIHSSNGYLFHQFFARCSNVRDDEYGGSHANRARLLFEVLD
                     AVSRELPLGRVGIRLNPMMNTLHGIVVDEDTVPFFDDLVRTADGLGLAYLHLTEPFQP
                     GQLDGVARPLDAVAAHFRAFTRTPILVNGGLSPQRGAELVAQGTADGAVFGRLFISNP
                     DLPARLQAGAPLAEWDMDTFYQGGEKGYLDYPRWEAA"
     gene            10480..10989
                     /gene="napF"
                     /locus_tag="PLH85_07285"
     CDS             10480..10989
                     /gene="napF"
                     /locus_tag="PLH85_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HNB64401.1"
                     /translation="MDITRRRFLGGRIGRNPGPAPFRPPWSLPEAAFLNQCTRCDDCV
                     KACPTGLLRRADDGFPVADFSAAECTFCGDCVPVCPPGALVRVGDAAPWSFGVRIGEG
                     CLALQQVECRICGEVCDPRAIRFRPRPGGIAPPEVDNTSCTGCGACIAPCPVVAVTRI
                     PLTTAPEAP"
     gene            10986..11276
                     /locus_tag="PLH85_07290"
     CDS             10986..11276
                     /locus_tag="PLH85_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HNB64402.1"
                     /translation="MNIVSLVLRIHPDTRAEAEAALTAFPGVECHGMSDDGKLVVTVE
                     DAEGASMSDTLIALHRVPQVLAATLAYEYTDRTAATPTHAPNSNSPCEEAQS"
     gene            11273..13828
                     /gene="napA"
                     /locus_tag="PLH85_07295"
     CDS             11273..13828
                     /gene="napA"
                     /locus_tag="PLH85_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HNB64403.1"
                     /translation="MTINRRDFIKAQAIATAAATAGISLPVVAAPKEAPAAAGGDTRV
                     RWDKAACRFCGTGCSVLVGVQDGRVVATQGDPDAPVNRGLNCIKGYFLSKIMYGEDRL
                     TKPLLRMKDGKYDKNGEFTPISWNQAFDIMAEKWKAALKAEGPGSIGMFGSGQWTIWE
                     GYAASKLMKAGFRSNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDIEQADAFVLWG
                     SNMAEMHPILWTRITDRRLTKTDVQVHVLSTFEHRSFELADNGMIFVPQTDLAILNYI
                     CNHIIQSGKVNEAFVKAHVNFKAGETDIGFGLRPAHALEKDAKFNGYPGADGKPKNNP
                     NDAKPITFDEFKKFVSTYTLDYTTKLTGVPAERLKKLAELYADPKKKVVSFWTMGFNQ
                     HTRGTWVNNMIYNVHLLVGKISTPGSGPFSLTGQPSACGTAREVGTFAHRLPADMVVT
                     NPDHRHHTEEIWQLPEGTINEKIGYHAVAQSRALKDGKLKCYWTSTTNNMQAGPNVNQ
                     EIYPGWRNPAAFVVVSDVYPTVSALAADLILPSAMWTEKEGAFGNAERRTQFWRQQVK
                     APGEARSDLWQYMEFSKRFKVEDVWPAELIAKKPEYKGKTLYDVLYANKVVNKFQKAE
                     TVKVNEHAIKNYTNDESEAFGFYVQKGLFEEYALFGRGHGHDLALFDVYHKARGLRWP
                     VVDGKETLWRFREGYDPYVKAGEDIKFYGHKDGKAVIFALPYQDPPEKPDADYDLWLC
                     TGRVLEHWHTGTMTRRVPELHKAVPEAQVFMHPDDAKKRGLQRGMQVKVQSRRGEMLA
                     RIETKGRNKPPVGLIFVPFFDESKLVNKLTLDATCPISKETDYKKCAVKVVRA"
     gene            13906..14823
                     /gene="napG"
                     /locus_tag="PLH85_07300"
     CDS             13906..14823
                     /gene="napG"
                     /locus_tag="PLH85_07300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HNB64404.1"
                     /translation="MDPVRNAARPPGRPAESPASAARRKFLNGIAAAAGGGCLLALGA
                     GLYSGSASALPAQALRPPGALAEADFLAACVRCGLCVRDCPYGTLRLADLGEAVPVGT
                     PFYTARRVACEMCEDIPCVKACPTGALDHGLTDINRARMGLAALVDHESCLNFLGLRC
                     DVCYRVCPVIDKAITLETQHNPRSDRHAMLLPTVHSDACTGCGKCEKSCVLPEAAIKV
                     FPRKLAQGALPAHYRKGWEEKARHGGSLIGDQVELPIRGFEGSTAGRAPTQTVVAPTA
                     PAPALPAPTADLPRPRTPAGSGINSHWKP"
     gene            14826..15695
                     /gene="napH"
                     /locus_tag="PLH85_07305"
     CDS             14826..15695
                     /gene="napH"
                     /locus_tag="PLH85_07305"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HNB64405.1"
                     /translation="MATAPSCQLSASPSADRRSLWQRQRWLILRRLSQASILLLFLAG
                     PWFGLWLVKGNLSSSLTLDVLPLTDPLLLLQTLVTGHVPEATAFLGAALVTGFYLLVG
                     GRVFCGWVCPVNIVTDAAAWTRRRLGIKTGRVPDAATRLWLLAGVLGVAAVTGRLAWE
                     WVNPVSLLHRSLIFGLGGGLWMMAAIFLYDVLVASRGWCGHLCPMGAFYGLLGQSALV
                     RVSAHRRKACDDCMDCFAVCPEPQVIRPALKQAGQTHPLILSADCTTCGRCIDVCDKQ
                     VFKMTHRFDQRSE"
     gene            15695..16180
                     /locus_tag="PLH85_07310"
     CDS             15695..16180
                     /locus_tag="PLH85_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HNB64406.1"
                     /translation="MNTPKIQTLRLRAAALALCLVHVLGVGILGAPAQAADAPVKLQS
                     LRGAEVPASDPEGDGARYGRDQPPMPRDFVQQPPLIPHTIRGYTITKNFNKCLDCHAW
                     SRAKETGATKISVTHFKDREGQELSSVSPRRYFCSQCHVPQSDAKPLVGNTFRPGVGM
                     R"
     gene            16205..16852
                     /locus_tag="PLH85_07315"
     CDS             16205..16852
                     /locus_tag="PLH85_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNB64407.1"
                     /translation="MNNTTGLIGRLRTTGWGLAALIFIAGIVFWGGFNTVLELTNREA
                     FCISCHEMKDNVYQEYRNTIHASNRSGVRATCPDCHVPKDWIPKIIRKIQASNEVLHK
                     VLGSIDTPEKFNAKRLELAKHEWERMKKTDSRECRNCHNYDSFDYAEQGRRSSRMHQT
                     GLNEGKTCIDCHKGIAHQLPQVEQSIGAPKEGATPEEFHPAPAAPAPATAPAEKS"
     gene            17084..18787
                     /locus_tag="PLH85_07320"
     CDS             17084..18787
                     /locus_tag="PLH85_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64408.1"
                     /translation="MSSPNPPGSPSSRRLHGPIVALVIALIVLIWGAILYKIHTEEEL
                     VIHSVNVENLNLARAFEEHTIRTLKSVDQAVLFLKFQYEKYGDKVDIGEYVREGMIIS
                     TIFNQLGVIDEHGIYILSNLPNHKKIDLSDREHFKVHMERDTNQLFISKPVLGRASKK
                     WSIQMTRRINKPDGSFGGVVVISLDPMYFSDFYKGVDLGTHGAVALVGLDGIVRARRS
                     GNNDSVGQDISASPFFTQTNTGTTGSFRSTSISDGIVRLYSYRALKDYPLAVIVGAAE
                     DEALQEFRGRRDGYLAYAAGMSLLVLFFGGVAIRLLNRQTQISDALDEARVRAESANR
                     MKSEFLASMSHELRTPLNGIIGYAEYLRDTLEGPDKEFAQTIHESGEHLLELVNSILD
                     LARVESGKMTLEFQEEMPRSLIDKVVRAQLPTAEAKGLQLETRFADDLPDHIVCDGTR
                     VVQVLNNLVNNAIKFTDQGQVTVTAGRDGKMMRVAVKDTGCGIPADMQDSIFERFRQV
                     EGFLTRRQQGSGLGLALVKELLTLMGGSVTLESTPGQGSEFIIRIPFTPARPDPLGHA
                     S"
     gene            18784..19140
                     /locus_tag="PLH85_07325"
     CDS             18784..19140
                     /locus_tag="PLH85_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64409.1"
                     /translation="MKVLVVDDQRVNRTLPAVLLRQQGCEVLEAESGEDALRLIDSHG
                     FQAILLDVSMPGISGTELCRQLRSRPEFGGVRIIAYTAHAMPGEREDILSAGFDDILI
                     KPINRQVLLAALRLPA"
     gene            19222..20127
                     /locus_tag="PLH85_07330"
     CDS             19222..20127
                     /locus_tag="PLH85_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNB64410.1"
                     /translation="MWFRNLQLYRLPENWDLSADTLADKLAARPFQPCGSQDMASRGW
                     TSPTRDDRFVLAVGGHYLIALAVEQKLLPSSVVNQVAADKAELIEAEQGYKPGRKQMK
                     EIKEAVLQELLPRAFTRRRTMWAWIDPKGGWLAVDAASPARAEDVLDALRDTLDALPV
                     KLLKTQQSPVSAMTGWLAGGEAPGAFTIDQDCELRAVTDEKAAVRYVRHALDGKDVQD
                     HLAAGKLPTRLALTFDDRIALLLTEKLEIKRIAFLDVVKEEAERQAETADLQMEADFA
                     LMTGELSRLIPALLEALGGEQKDAF"
     gene            complement(20177..20755)
                     /locus_tag="PLH85_07335"
     CDS             complement(20177..20755)
                     /locus_tag="PLH85_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HNB64411.1"
                     /translation="MSGDHFHVHGPHDHAVEHAAEHGGGDPFASRIAAMTAILATLGA
                     LFSYAGGATQNDALMHKNEAAIRKTEASNRWGYYQAKSAKQNLAELGVSLTTGEIQTR
                     YTAEIERYKQEKQEIKSAAEELEKAAIEAEEASEHAMHAHHRWAQAMTAMQVAISLAA
                     ITLLTRRRWMQWTSVGVATAGCAIAGLALAGI"
     gene            complement(20915..21094)
                     /locus_tag="PLH85_07340"
     CDS             complement(20915..21094)
                     /locus_tag="PLH85_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64412.1"
                     /translation="MLPTPFERIKDLIGKIHALEDKLMTTGILPLSEQAKIHEQIRHY
                     KEEIALLERRTRKHS"
     gene            complement(21180..21767)
                     /locus_tag="PLH85_07345"
     CDS             complement(21180..21767)
                     /locus_tag="PLH85_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64413.1"
                     /translation="MAVRPDRDAASYDRRIADPVPDHPLLAGLAELGRGESSIVLDAG
                     DGERVFKVVSSPADYCFLAADDRPIGLHFPRLIKDHGVVGKASNGYSFRLLELERLLP
                     VAGEAEALGQLLSDAYWQGCEKWANLGVNRGRLALYHIAQEPPPGLPTSLVKAVADLS
                     DFIEGYPVQPDILNPHNLMMRPDGTLVFSDPVFLP"
     gene            complement(21776..22555)
                     /gene="fabI"
                     /locus_tag="PLH85_07350"
     CDS             complement(21776..22555)
                     /gene="fabI"
                     /locus_tag="PLH85_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNB64414.1"
                     /translation="MQPIINLAGKVGLITGIANEKSISTGVAEACIEAGAKVVITYQN
                     DKTLAFIEPIVARLGIEDVYLLDVTKPETMDDVFAQIDAKHGKLDFAIHSMAFAKRDD
                     LHGRVVDTSPDGFALAMDVSTHSFVRLARKAEPLFEKAGGGSLITMTYLGSEKVIANY
                     GVMGLCKAALEAATRYMAFELGPKNIRVNAISPGPLMTRAASGIAGFDGLMAAAVERS
                     PLHRLVTPEDIGALTAFLVSDAGRNLSGGVHFVDAGYNIIA"
     gene            22694..23200
                     /locus_tag="PLH85_07355"
     CDS             22694..23200
                     /locus_tag="PLH85_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HNB64415.1"
                     /translation="MHQTEVSPPAAAGPGPVVVFLDFDGVTHPWGESEDFRCLPVFEA
                     VLRDYPEVRVVIASDWRTLFSLPKLTARFAEDLRARVVDVSPTIFPKEANELYGLRER
                     EARQWLARHPDIAGWLAVDDAPGNWPTRSRLVLTDFKRGFTEEDAGRMRRMLDGFRRG
                     EYPEQAAA"
     gene            complement(23268..24272)
                     /locus_tag="PLH85_07360"
     CDS             complement(23268..24272)
                     /locus_tag="PLH85_07360"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNB64416.1"
                     /translation="MSAGDGAAAPAPFGPLTPDFVLAAVESAGLFCDGRLLTLNSYEN
                     RVYQVGIEDAAPLVAKFYRPGRWSDAAILEEHTFLAELAAAEIPAVPALELDGRTLHA
                     FEGFRFALFPRRGGRAPELDDPAVLTWLGRFLGRIHAVGATRPFVHRPDLTPRSFGEE
                     ARDALLETAFIPAELRATWQSVVDQALAGVHACYARAGELPTIRLHGDCHGGNVLWTD
                     AGPHFVDFDDARNGPAIQDLWMLLSGDAPAMARQLDHVLEGYEAFADFDPRSLHLVEA
                     LRTLRLIHYAAWIARRWDDPAFPAAFGWFDSPRYWQDMILALREQVALMDESPLTRQF
                     "
     gene            complement(24269..25030)
                     /locus_tag="PLH85_07365"
     CDS             complement(24269..25030)
                     /locus_tag="PLH85_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNB64417.1"
                     /translation="MEFLTDPQLLIAFLTLTALELVLGIDNVIFISILVDKLPPERRA
                     FTRRLGLFLAMFMRIGLLMILSWLAGLTEPLLTVLGHGFSGRDLILLGGGLFLVWKST
                     MEVHQLMEGEEGEASSTVQATFGAVLLQIVLIDIVFSLDSIITAIGMVNNLTVMIAAV
                     IASVGLMMVASGPIGDFVSRHPTVKMLALSFLLVIGVVLIADAFGQHVPKGYIYSAMA
                     FSVMVEMLNLRMRSRQAAEPVKLHEPYVREPGRDA"
     gene            complement(25177..27489)
                     /locus_tag="PLH85_07370"
     CDS             complement(25177..27489)
                     /locus_tag="PLH85_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB64418.1"
                     /translation="MKSAADSQTGPGASREDALSPDATLFEALSLFLAGGPSYLPVGD
                     AGPARRYLGAATLLGALHAGLSGDTPVSRLNLDTSADSATAAGSAKTLIDALPLLLWM
                     KDAEGRYLIVNRSFAEACGRSTSAEVIGRADDELWPAGRAALHAAADAEVLAGGEAMR
                     AVDQVSGGASPVWLESVRAPLRGAEGGIVGTVGFAVDVSERIRLLAAAERSEKDLNFV
                     LSAIGEGVWDRDLASGTVRHNRRWCELLGLGHAQLIHSVDDLLAMIHEADRPLYNAAV
                     AACMAQHGAYICEYRMRRPDGRVLWVRDRGDVVEFDADGQPSRMMGSIADITEVKESQ
                     RHLEHMAYHDTLTQLPNRSLFYDRMQMAVAQAERNGTLAAIAYLDLDGFKAVNDSLGH
                     AVGDRLLVVIARRLMASTRAGDTVARIGGDEFALIYNGLERSEECEQILQRLLASIGE
                     PVTIDNRELTVTASVGVTLCPLDGSDPEHLLLHADEAMYLAKESGRNAFQLYDPGRDR
                     RLRALRDAQHRVELALDGGELELHYQPKVDVLTNALTGVEALIRWKHPELGLRMPGEF
                     LPAVKDSRFEIELGDWVLRTAVKQLDAWHREGLSTPMSVNIAARHLVHPEFVANLRRL
                     LAAYPAVHPELLQLEVLETATLGDVKRVAGIMAACNELGVSFAIDDFGTGQGALSSLR
                     RLPVRSIKIDRSFIHNMLEDEEDLATVQSVIGLGAAFHREVIAEGVETPGHRQALLRL
                     GCRMIQGHGVARPMPAARMQRWMQEHRTQH"
     gene            27765..28457
                     /locus_tag="PLH85_07375"
     CDS             27765..28457
                     /locus_tag="PLH85_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB64419.1"
                     /translation="MSPDKIDIPGLLSRMPMFSALSPDDLAHIAAATRERRLDKGERL
                     FQRGDAPKGFFLVVWGQVKLAFTSSQGNEKVVEILGPLQSFGEAVMFLDQPYPVLAEA
                     LADALILHIGSAAVFGLIDADPKFARKLLAGLSIRLHSMVRDVESYALRSSTQRVIGY
                     LLQLAESTPCAAQGERSVELPTSKQVIASRLSLTPETLSRIFHELSGAGLISVHGKQI
                     VIHDAERLAAHD"
     gene            complement(28468..28704)
                     /locus_tag="PLH85_07380"
     CDS             complement(28468..28704)
                     /locus_tag="PLH85_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB64420.1"
                     /translation="MSETLAPVIVDARGLMPPQPMHLTLEALDTLPAGGEVILLLYRE
                     PTPLYDVLIRNGYVYRAESTPGGEFAIHIRHRTD"
     gene            28857..29387
                     /locus_tag="PLH85_07385"
     CDS             28857..29387
                     /locus_tag="PLH85_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64421.1"
                     /translation="MKEGTVLLVSCAATAALWLWAEQNRPAPALPEAAPTPSAEAPPL
                     ASPPVAPPSPDSRAYLCIANGQRSYSDRPCAAGERSERVNIQAPPPGSPTPSIQEQYA
                     DLVANRPRVTPGAAEPLVIPRDPDHPEKAGLCEELWLHIREIDEALRQPTPPQQAETL
                     RALRLRYQNDRFTQGC"
     gene            complement(29517..31076)
                     /locus_tag="PLH85_07390"
     CDS             complement(29517..31076)
                     /locus_tag="PLH85_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNB64422.1"
                     /translation="MKRLLASLAFAATLLPTVAAAVVDAPALYTQHCASCHGPGRLGA
                     MGPALLPESLERLRRADAIATLRDGRTATQMPAFGATLSAAEIEALADWVRTPVSPAP
                     AWSEDDIRASRVEPFPRGSLPDAPQGPVAQTDPMNLFIVVEAGDHHVSVLDGDRMTRI
                     HRFPSRYALHGGPKFTPDGRYVFFASRDGWVSKFDLWNLKVVAEVRAGLNTRNAAVSA
                     DGRFVAVANYLPGNVAIFDADLKLLRLIPARTLKGDASSRVAAVYDAPPRRSFIVGLK
                     DVAEIWEVSYDPDAKPIFDGYVHDYKMGEAMALPGFLNSRRTALDTPLDDFFFTQDYA
                     NVVGASREGRGEVVNLDVRRAIATLDLPGMPHLGSGISWMRDGRRVVASTHLKEGRVS
                     VIDLQRWQTVATIPTLGPGFFLRSHENSPYAWVDSMMSPTAKDTLQVIDKQRLAVVAQ
                     VRPEPGRTFAHVEFDRHGRYVIASLMENDGALIFLDATTFQEIKRLPASKPIGKYNLF
                     NKIGKSEGTSH"
     gene            complement(31468..>31701)
                     /locus_tag="PLH85_07395"
     CDS             complement(31468..>31701)
                     /locus_tag="PLH85_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HNB64423.1"
                     /translation="LSDPLLAGLKQSPRPVSGRCGACRYLAMCNGNTRVRAWQLTGDP
                     WQEDPGCYLDNAEIGVADGERLEVKPWVRLQPA"
BASE COUNT         5415 a        11343 c        10366 g         4577 t
ORIGIN      
        1 agggtcatca cgctgccggg gtcggtgagg gcctggcgga tggcggcttc ggcggcggcc
       61 tcgtcggcag gcagccgcgc cgggtcgggg cgctcggcga gcaccgccgc caccagccgg
      121 cggtagcgcg cggccaggcc cggaaagcgc gccagggtgg cggcgctggc ggtctggttg
      181 gcgacgatcc agttgcccgg cagctcgggc agcgcggcgc cctgggcgat cagccacagg
      241 tacaggtcgc ggttgaggcg gcgctccggg aacagggcga tctccgccgg caggcgcagg
      301 gtctcgtcgt cgcgggccgc gtgggcgacc ttttcacccg cgtgggcgat gcgcgccagc
      361 cagctgcgcc gggcgccatg ctccacctcg gcggcggcgg ccacacgcag gccggaatcg
      421 ccgcccaggg cgcggaacag gatgcccgcg gtgcgttcga tctcgctgag cctgaccgct
      481 gcctcgggat ggctgcggga cgcggcgcgg gtgatgaagc ggtgccatag cccgccgatg
      541 aactcttcca tgacccgtcc tatccgaaga tccacccgcc atgactgccc gccaccgcgc
      601 ccagcacggt gaccaggctg gccgcgagga gcaccccatg cagccgccgg atgcgcgcga
      661 aggtggcgtc cggatcggcc tgggcgagct ggcggaacag cttgtggagc acgaagggct
      721 ccagcacgaa gagcatcagc gcgaagaccg tccacaccgc caccatggcg tgcatccacc
      781 accactcggc gggctgggcg aagcggctcc acagatcgag ccgccacacc aggtagaggc
      841 cggacgcgcc cgccagcagc accgcagcgc gggcgatggc ggcgaaacga tgctccagac
      901 gctcgaacag gtcgatgcgc gcggccggct cggccagatc gcggcaggcc ggcagcagca
      961 cccaggtgac gaaggcgacg ccgccgatcc agatcagcac ggccagcacg tgcagggcgc
     1021 gggccagggc aaagtcgttt aaaccgctca cggatgcttc ctccattctt cggcacgggg
     1081 cgcgcggaat gccgcctcgt tgtggcgcgc cgccgcgccg ctcacccagt gcagcagggg
     1141 gccggacggg tgatcccgct gggtggtggc gcaggcgctc acgcactggg cgcactgggt
     1201 gcaggtgaac atggcgcgct tgatcgcccg cggcttgagg cgcatcgggc agacgtgctc
     1261 gcaggcggcg tcgcacccga cgcactgggc cgcgcgggcg cgctcgaagc ccaccaccat
     1321 ggcgtcccgg ttacccatcc aggccacgct ctggaacatc cccaccgcgc aggcgaaacg
     1381 gcagaataaa tgccgcgcga agaggaactc ggcgctcagc agcaccgtgc cggcgccgat
     1441 gaagagcgcc tcgttacggg tcggcgtacc cgccagcagg tggccataca cctggaaggg
     1501 cggcagcaga taggtgagca gcaccaccgc ccagacgaag gcgaaggcca ccgccagggg
     1561 cacggtggcg ccccaccagc gcggatccac cggcaggggc gagccgtccg gcgcccgtgc
     1621 cggcagggcg tggggctccc acacgctggg cttgccgctg gcgcggcgca tcagcccgtt
     1681 gatcgtctcc accaccgaga agtgcggaca cagccagccg cagtacagcc ggccccagcg
     1741 ccaggccacg ccgatcagca ccgcgccggc cgccagaatc ggcagcagga cgcgcaccag
     1801 cacccgcagg ccggcctcca gggcaccgat gcgcccggcc gagaagtctt ccagcccggc
     1861 atgccagggc atccccagga accaggcgtg gccggccacc aggtcatagc gcagcaggtc
     1921 gaacaccggc gccagcacga acagcacgaa gaaggcgcct tgggcgaggc ggcgctgggc
     1981 ctgacgcccg ccgtccggtt gcatcgcggg cacggcgtgg atcgggatca cgcgggccgg
     2041 gttcacgcga cctcccgcgg gcccaccacc ggaaagcgga aggaccgccc cgccagcgcc
     2101 cgggacaggc ccaccgcgcc gcacaggatc agggacgaat ggaccagcgt gaaatagagg
     2161 atgagcacca cccaggtaga cgcggcgcgg tagccgccca aaagcaggat cgccccgttg
     2221 gcgacgatca gcagggccac ggcccacagg ctcgccgcca tcgtctgacg caggtggctg
     2281 cgcgccaggg gcggcgcgtc ccgatgacgc aggctccaca gcacggccag gacgatgaag
     2341 ccgaggccgg ggatcagcat cagattggcc aggtagaggg cctcggcggc caccgccagc
     2401 tcgcgtccgg ccacgtccgc atcggcggga tcgacgggga gcatgtgcgg cgcccgcggg
     2461 cagtgcgtgc cgcgttcagc gcccgaaggt ggcctgcacc acctcgagca gcgcgtcggc
     2521 cacgtcggca tcgtcggtga ggctttccac cagtgccgag cggcaggcct ccaccggatc
     2581 caggcccacc ttcaccagct tggcggcgta aaccagcagg cgggtggaca ccacctcctc
     2641 cagatccacg tccttcagcg cccgcaggcg gccggcgatg gacaccaggc gcttggccag
     2701 catcgggtcg cagccggtct cgccgatgac gatggcctct tcccgcgccg ccgcggggaa
     2761 ttcgaaacgc aggctgacga agcgctggcg ggtggacggc ttgaggccct tgagaaagtt
     2821 ctggtagccc gggttgtagg acatcaccag catgaaggca tccggcgcgt ggagggtctc
     2881 gccggtgcgc tcgatgggca gcacgcggcg gtcatcggcc agcgggtgga tcaccacggt
     2941 ggtgtcacgg cgggcttcca ccacttcatc caggtagcag atgccgccct ggcgcacggc
     3001 acgggtcagg ggaccgtcac accacacggt ctgaccgtcg cccagcaggt ggcggcccac
     3061 caggtcggcg gcggtgaggt cgtcgtgaca ggccacggtg aacaggggca ggcccaggcg
     3121 cgccgccatg tgcgcgacga agcgggtctt gcccacgccg gtggggccct tgatgaggag
     3181 gggcagacgg ttcttccagg cctgttcgaa gacctggatc tcgttgccgg ccgcttcgta
     3241 gaaaggaaga ttggcagtca tgggtgcgtt catcggagtg cgatccagta ggcgagggcg
     3301 atcagccccg cgacgacgag cagccagcct aacaggagtg cgcgccagaa gaacttgacc
     3361 ccgcgcaaac ccatgaaatc gagcaccacc aggcgcccct tgacgaagct gatggccagc
     3421 agcgccgcca tggccgcggg accggcagcg ccgtgctcgc ccagttccca ggtgagcgcg
     3481 gtggccaccg cgaggacgac ccacgcgaag ttgagctggc ggacgttcat cgcatcacgt
     3541 acaccagcgg aaacagcacg atccacacca gatccaccat gtgccagtag gccgcgccgc
     3601 tctccaggcc gttcatgcgc cccgcgccgt aggccccgcg acgggtctgc acccacagca
     3661 cggcgaggat caccagcccc aggatcacgt gcatgaagtg aaagaaggtg agggacaggt
     3721 agaacatgta gaaggtgttg gtggacaggt tgatgcccgc cccgaacttg gccgcgtatt
     3781 ccacgccctt cacgcccagg aagccggcgc cgcagaccag accgcccgcc agccagcgcg
     3841 cggccgtggc ggaacgatcg cgaccggccg cctccacggc gcgcgccacg cagaagctgc
     3901 cggtgatcag gagcagggtg ttcaccgcgc cggcgcgccg gtccagggtg gcctgcatgg
     3961 aatcgaagag atcgggatga cgggcgcggg caaaggcgta ggcggcgaag aacacgccga
     4021 aggccagcag ctcggccagg atgaagaacc agatcgccag gtcgccgggc ggcgccgcgg
     4081 caggcgcggg gcgggcagat gtgctcatgc ccggcagtgt cgccggagcg tgccaccgga
     4141 gccttgatct ggcataagac gcgcactctc ccggcgtcgt aaattcctgt cacacgcccc
     4201 ccgctgatct atagctttag tcgtagatgt cgttatggca gggagattct tggacccctc
     4261 gcgcggaggc gcgccgatga aaattcttct tgtggacgat tcccgggcgg tggccgccgt
     4321 gatgggcgcg cgtctgagcg gtgccggtta cgaggtgatc ctcgccgaga acggcaaggt
     4381 ggcggtggag cagttccgcg ccagcgcacc cgatctggtg ctgatggaca tcgagatgcc
     4441 ggtcatgaac ggcttccagg cggcccaggc gatccgcgaa ctggaagcca ccggcgaatg
     4501 ggcgtggacc ccgatcattt tcctgaccgc ctccgacacc cccgaaaacc tggtcactgc
     4561 cattgaggcc ggaggcgacg acttcatcgc caagatcctt ccggaagccg tgctggaagc
     4621 caagatgaag gccatggcgc gcatcgcccg cctgcgcgat cagctctccg ccgccaatcg
     4681 caagctcacc gagcaagcca gccaggacgg tctcaccggc ctgtgcaacc gtcgctacat
     4741 ggacctgcgc ctggacagcc agtgggccgg ctgctgcgat gcgcgtgagc ccttcgggct
     4801 gctcatgctg gacgtggacc acttcaagaa atacaacgac cactacggcc accaggccgg
     4861 tgacgactgc ctcaaggccg ttgcgcgggc catctccgag accgtcgaag ccgccaacgt
     4921 gcggggcgac gtgcatggcg ccctggccgc ccgctacggc ggagaggaat tcgcggtgct
     4981 gatgccccgc tgcacgcggg acacgttccg ggccctcgcc gacgcgatct gtacggccat
     5041 ccgcacactc gcgattcccc atgaacgcaa cgcggaatgg ggcatcgtca cggcatcggt
     5101 cggcggcgcc tacgccgcgc aggcggaagg ccgcgtcgcc ctgcgcttcc gggaagcaga
     5161 cgccaacctc taccgggcca aggatctggg ccgcaaccgg gccgtactgg actgacgtcc
     5221 ctgccggccc gtcaatagag ccacggaatc agccgccggg tgcgccgacg atagtcggcg
     5281 taggccggaa agcgatccgc cagccagcgc tcctcccggc gtgacttcac atccagcagc
     5341 acgaacagca gggccgccca gccgagggtc agccagccct ggtggaagag ggcccaaccg
     5401 aaggccgaga ggatcagccc cagatagatg gggtgccgga cgcgcgcgta caggccgctc
     5461 accaccagcg tcgcgccgtc cttgggatgg ggcagggggg tgaggttcga acccaggcgc
     5521 agggccccca gggcggcgat gagaccacct gccgccatca gcgcaattcc gccgatcagc
     5581 gccggcagcg ccgcctccgc cggccagggg ggcagacccg ccacggtgcg cggtccgaac
     5641 acgagcatga ccagcagcgc cacctggatc atcacgtagg cctcgccgcg caggccctcc
     5701 cagcgtactg tcggaccgtt caccgcatgc tcccttctgt cgccataccc ctctccacgt
     5761 ccatgcgccc cttgaaagcc agcgcacgcc gctccacaca gatccacgac agacccgcca
     5821 gcgccaccgt cagcggtgcc gagactgcca gcagccccac cggcgtgatc cctggcaaca
     5881 gcgccacgac ggtctgctgc acgggaaagg cgtacaggta gaccccgtag gagcagtctc
     5941 cgacgcggtt gaagcgtcgg atcacgccgc ccggcacatg cgccagcgcc accaccagat
     6001 agcccagcag cagcacgtag gccacgaaga acacgtcccg ctgcagactc gccagcgcca
     6061 gcaccccgcc tccggccaac aggccggacc accccagggg tacccgtgcc gccgccatct
     6121 gcaggcacgc accgcagaag aacatccacg cgaagcgggc aaagtagccc gcatccgtat
     6181 gaaagaaatg gcggaccagc atcgtcccgc ccgccacccc cgcaatcagc gccacggcgc
     6241 ccgtcagcac gccctgccga cgccgaccga gacgctgcac gacgatgccc agtaaggcca
     6301 ggatcgcgta catgcgcagc tcgtaaggca gggtccacag ggatccattc accgcagccg
     6361 gatagggatt ggcctcgaac accccgggca gatggatcgc gatgccgccc cacaggctgg
     6421 cacatttgat caggtagtcg accaccccgg ggtcggccca gtaggccgcc gccgggcgcg
     6481 tcgtgagcag tggcccgagc acggccacgg tcagcaccac caccccccac agcgccgggt
     6541 agatgcgcag cacccgtgcc cacacaaagg ccctcacccc cggacggtca cgcaggctgg
     6601 ccgtcaccag gaaaccgctg gtcacgaaga acacatccac ggcgacgctg ccgatgtcga
     6661 tgcccagcca gtccgccaac ggcggcgccc cgcctcctgc gagggggtag caatgcccat
     6721 agagcaccgc cagggccgcc aggaagcgga tcaggtggaa attgttgtcc cgcccgcggg
     6781 ccgcctcggc cagcgtcacg cgagctccgg ggggccggac gacgcggtcg tcagtccgct
     6841 gcaatcattt cgcattgcac cattgtagcc gctgcaggca aggctgcatg caatgcggga
     6901 ggctgcggga aagaaacctt acaatgcagt gacagacacg tcgcgcccgg gacaagggcg
     6961 cacgaccaca ggaggagaca ccatgggcgc catcttcgcc aagacccgca aagggcagca
     7021 ggaaatcgag acccgttccg gcgggctcca cccgcgggtg cgtcgcaccc tcatcgtcgt
     7081 cgacggcaag cgcagcgtcg acgagctgcg cgaagcgctg cggagcgacg acctgcagca
     7141 caccctcggc ctgctcgaag aggagggcta catcgagctg atctccggcg cacggctcgc
     7201 ggaggctgca ccggcgtcca tcagcgcctt ccggctccat ccgccggggg aagatccgct
     7261 gcgcctgcag cagagccgca atttcatgct caacaccctg cgcaccttcg tcggcgcggt
     7321 gggcaccagc tccctgcagg accgcctcga ggcggcgcgc acccatgccg acctgcgcgc
     7381 cctcttcgac gagtggtacc acgccctggt cacctcccgg gatggccgcc gcgaggccga
     7441 acacctgcgc agcaagctcc tcgagatcct gtaggccagc cgcgctcagc tccccttggg
     7501 gggcaggccg gccacataaa ggcgggccgc ctccacccag gccgccagat ccgcgtcctc
     7561 ggcgaagccg tccggtgcca cgtagacata gccacgcaag ggtcgtccgg tgaagtccat
     7621 cggccgggca tgggggcggg cgagcgcggc ggcataggca tccggtccga cccggaccat
     7681 cagcgtctcg ccgacgatgc cgacacacat gtgaccccgc cacatcagcg cgagcccgcc
     7741 gaacatgcgc cgctcgccca cgtccgtcat tccggcgaac gcctcccgca gacgctccgc
     7801 cagcccttcg tcgaatgcca tcccgccctc cgcccgtcct gacgtctgct tacagttaag
     7861 ggggcacttt cggcgcccgt gaggctcgaa ggcctcgcca tccccttctg cgaggcctgc
     7921 gtcgatgttc aagaagctgt ccgccctcta caccctgatc cgcgaagacc tgcgcatcct
     7981 gggcttcgcc ctgcgtcatc cggcgcgtcc ggtctggttg ctgccagccc tcgtggcgct
     8041 ggggctgtac gtcctgtcgc cgatcgacct gctgccggac ctggtgccgg tactcggctg
     8101 ggtggatgac ctgatcctgg tcccccttgt catcgcctgg ctggtgagcc ggctgccggt
     8161 cgacctgcgc cgcgcctggc aggatgcccg gcggaccgtg gatgcgcccg ccgtgcgccg
     8221 cgcctgaagc ctcaggcgcg caccaccgcc gccgaaaaga gctggagcac caccaccccg
     8281 gcgacgatca ggccgatgcc gatcagcgcc gggcggtcca gcttctgacc gaagaaataa
     8341 cgcccgacca gggtcatcag cgccaccccg gagccggacc acaccgcgta caccaccccc
     8401 accggcagca cgtccagggt gagggacagg agatacaggg ccagcgaata gcagctcacc
     8461 acgatgcccc agggccacag gcgcctgaaa ccgtcggcca gcttgagggc cgaggtagcg
     8521 atgacctccg cgacgatggc gacgaagagc agcagccagt ccatgtcagc ctcccgccgc
     8581 gttcattcgg tcaccgtctc ggagaaggcg ttgatcacca ccacccccgc cacgatcagc
     8641 ccgatgccca gcagccccgc cggatccagc gtctgatcca gaaagaccca cgccaccacg
     8701 gtgatcagcg ccacacccac gccggaccac accgcataca cgatgcccac ggggattttc
     8761 ttgaggctca gggacagcag gatgaaggcc gtctcgtagc acaccacgac gaccacagac
     8821 ggccacaggc gggtgaagcc ttcggtggcc ttgagagtcg acgtggcgat cacctccgca
     8881 acgatggcca cgaagagcag cacccactgc agggtgccgg cccggggcgc aggggccggc
     8941 gtggcggtcg gatcgggcgc agccatcagc gcgtcgccgc ctcgatgtcc ctgcggcgct
     9001 gctccgcctg caggcgcgca gcttcgagct ggctttccac ctgggccttg acggcctccg
     9061 cctgctgcgc ctcctgggcc ttgagcttgg ccgcggtggc cgcgctgctg ccggcatcac
     9121 caaagccgca ggccgccaag ccggtggcca gcaggccggc cagaacgaag gccctcatgc
     9181 ggcctcccag cgcgggtaat ccagatagcc cttctcaccg ccctggtaga aggtgtccat
     9241 atcccactcc gccagcggcg cgccggcctg caggcgtgcc ggcaggtccg ggttggagat
     9301 gaacaggcgg ccgaacaccg cgccatcggc cgtgccctgc gccaccagct cggccccacg
     9361 ctgcggcgac agtccgccat tgaccaggat gggtgtgcgg gtgaaggcgc ggaagtgggc
     9421 cgccacggca tcgaggggtc gggccacgcc gtcgagctgg cccggctgga aaggctcggt
     9481 gagatgcagg taggcgaggc ccagaccatc cgccgtgcgc accagatcgt cgaagaaggg
     9541 cacggtgtct tcgtccacga cgatgccgtg gagcgtgttc atcatcgggt tgaggcggat
     9601 gcccacccgg ccgaggggca gctcgcggct caccgcgtcg agcacctcaa agagcaggcg
     9661 agcgcggttc gcgtggctgc cgccgtactc gtcgtcgcgg acgttgctgc agcgggcaaa
     9721 gaactggtgg aacaggtagc cgttggagga atggatctcc acgccatcga agcccgcttc
     9781 acgggcatgg gcggcggcgc gggcgaagtc ggcgacgatg ccagcgatct cctcgcgggt
     9841 gagggcgcgg ggggtgaccg tgggcgtcag gcctgcacta ccgaacatct tccactgggg
     9901 atcgatggcc gagggcgcca ccggcgccgc gccgccgtgg aattccggca gggacaggcg
     9961 gccgcagtgc cacagctggc agacgatgcg cccgccggcg gcgtgcaccg cgtcggtgac
    10021 gaggcgccag cccgccacct gcgcggccga ccagaggccc ggcgtaaagg ggtagccgcg
    10081 cccctggggc gacacgatgg tgccttccga aacgatcagg ccggcgccgg ccctttgggc
    10141 gtagtactgc gcctgagtgg cgtccggcgc cgcctcccca ttggtcacac gacagcgcgt
    10201 gagcggcgcc atgacgatcc ggttggccag atcgagatcg cccagccgga ccggctggaa
    10261 gagggcggaa acagcagcag ggggactcat gacggggcct ttcgacgatg acggcagcac
    10321 gccgcgatgg tagcaaatcc ggatggcggg tggtcccgga ccgagtggga gatgtctgtc
    10381 cgtaagcttt caaggggctg gcaattcttt gcacagatca aggaaaccac ctggccgggt
    10441 gcctagactg actctgtttc gatccacctt ttcctgttca tggacatcac ccgccggcgc
    10501 ttcctgggcg gacgcatcgg acggaacccg ggccccgcgc ccttccgccc cccctggagc
    10561 ctccccgagg ccgccttcct caatcaatgt acacgctgtg acgactgcgt gaaggcctgc
    10621 ccgaccggcc tgctgcgccg tgcggacgat ggcttcccgg tggcggactt cagcgctgcc
    10681 gaatgcacct tctgcggcga ctgcgtgccg gtctgcccgc ccggcgcctt ggtgcgcgtc
    10741 ggcgacgccg ccccctggtc cttcggtgtg cggatcggcg aaggctgcct ggccctgcag
    10801 caggtggaat gccgcatctg cggcgaggtc tgtgatccgc gggcgatccg cttccgtccg
    10861 cgtcctggcg gcatcgcccc acccgaggtg gacaacacct catgcaccgg ttgcggtgcc
    10921 tgtattgcgc cctgtccggt ggtggccgta acgcgcatcc ccctgaccac cgccccggag
    10981 gccccatgaa catcgtcagc ctggtactgc gcatccatcc cgacacccgc gccgaggcgg
    11041 aagccgcgct caccgccttt cccggcgtgg aatgtcacgg catgtccgac gacggcaagc
    11101 tcgtcgtcac ggtggaggac gcggaaggtg cttccatgtc cgacaccctg atcgccctgc
    11161 accgggtgcc ccaggtgctc gccgcgacgc tggcctacga atacaccgac cgcaccgccg
    11221 ccactccgac ccacgccccg aattcgaatt ccccctgcga ggaggctcaa tcatgaccat
    11281 caatcgacgc gacttcatca aggcccaggc catcgccacc gccgcggcca ccgccggcat
    11341 ttccctgccc gtcgtggcgg cccccaagga ggcgcccgcc gccgcgggcg gcgacacccg
    11401 ggtgcgctgg gacaaggccg cgtgccgctt ctgcggtacc ggctgctcgg tgctggtggg
    11461 cgtgcaggat ggccgcgtcg tggccaccca gggggatccc gacgcgccgg tgaaccgggg
    11521 cctcaactgc atcaagggct atttcctgtc caagatcatg tacggcgaag accgcctgac
    11581 caagcccctg ctgcgcatga aggacggcaa gtacgacaag aacggcgagt tcacgccgat
    11641 cagctggaac caggccttcg acatcatggc cgagaagtgg aaggccgccc tgaaggccga
    11701 aggccccggc tcgatcggca tgttcggctc gggtcaatgg accatctggg aaggctacgc
    11761 cgcctccaag ctgatgaagg ccggcttccg ctccaacaac ctggacccga acgcccgcca
    11821 ctgcatggcc tcggcggtcg ccggcttcat gcgcaccttc ggcatcgacg agccgatggg
    11881 ctgctacgac gacatcgagc aggccgacgc cttcgtgctg tggggctcca acatggccga
    11941 gatgcacccc atcctgtgga cccgcatcac cgaccgccgc ctcaccaaga ccgacgtgca
    12001 ggtgcacgtg ctatccacct tcgaacaccg cagcttcgag ctggccgaca acggcatgat
    12061 cttcgtgccc cagaccgacc tggccatcct caactacatc tgcaaccaca tcatccagtc
    12121 gggcaaggtc aatgaagcct tcgtcaaggc ccacgtgaac ttcaaggccg gcgagaccga
    12181 catcggtttc ggcctgcgcc ccgcccatgc gctggagaag gacgccaagt tcaacggcta
    12241 ccccggcgcc gacggcaagc ccaagaacaa cccgaacgac gccaagccca tcaccttcga
    12301 cgagttcaag aagttcgtct ccacctacac tctggactac accaccaagc tcaccggcgt
    12361 gccggccgag cgcctcaaga agctcgccga gctctacgcc gacccgaaga agaaggtggt
    12421 cagcttctgg accatgggct tcaaccagca cacccgcggc acctgggtga acaacatgat
    12481 ctacaacgtc cacctgctgg tgggcaagat ctccaccccg ggctccggcc ccttctccct
    12541 caccggtcaa ccctccgcct gcggcaccgc gcgggaagtg ggcaccttcg cccaccgcct
    12601 gcccgccgac atggtggtca ccaacccgga tcaccgtcac cacaccgagg aaatctggca
    12661 actgccggaa ggcaccatca acgagaagat cggctaccac gccgtcgccc agagccgcgc
    12721 cctcaaggac ggcaagctca agtgctactg gaccagcacc accaacaaca tgcaggcggg
    12781 ccccaacgtc aaccaggaga tctaccccgg ctggcgcaac ccggccgcct tcgtggtggt
    12841 gtccgacgtg tatcccaccg tctccgccct ggccgccgac ctgatcctgc cctccgccat
    12901 gtggaccgag aaggaaggcg ccttcggcaa cgccgagcgg cgcacccagt tctggcggca
    12961 gcaggtcaag gcgcctggcg aggcccgctc cgatctgtgg cagtacatgg aattctccaa
    13021 gcgcttcaag gtggaggacg tgtggcccgc cgagctcatc gccaagaagc ccgaatacaa
    13081 gggcaagacc ctgtatgacg tgctctacgc caacaaggtg gtgaacaagt tccagaaggc
    13141 cgagaccgtc aaggtcaacg agcacgccat caagaactac accaacgacg aatccgaagc
    13201 cttcggcttc tacgtgcaga agggcctgtt cgaggaatac gccctcttcg gccgcggcca
    13261 cggccacgac ctggccctct tcgacgttta tcacaaggcc cgcggcctgc gctggccggt
    13321 ggtggacggc aaggagaccc tgtggcgctt ccgtgaaggc tacgacccct acgtcaaggc
    13381 cggcgaggac atcaagttct acggccacaa ggacggcaag gccgtgatct tcgccctgcc
    13441 ctaccaggat ccgcccgaaa agcccgatgc cgactacgac ctgtggctgt gcaccggccg
    13501 cgtgctggag cactggcaca ccggcaccat gacccgccgc gtgcccgaac tccacaaggc
    13561 cgtgcccgaa gcccaggtct tcatgcaccc ggacgacgcc aagaagcgcg gtctgcagcg
    13621 gggcatgcag gtcaaggtcc agtcccgccg cggcgagatg ctcgcccgca tcgagaccaa
    13681 gggccgcaac aagccgccgg tgggcctgat cttcgtgccc ttcttcgacg aatccaagct
    13741 ggtgaacaag ctcaccctcg acgccacctg cccgatctcg aaggaaaccg actacaagaa
    13801 gtgcgccgtc aaggtggtgc gcgcctgagg ctggctacgg cgaccggccg cgtgacgccc
    13861 ccttgcccac gcacgccggc cgcctgatcc gacatcggaa tcatcatgga ccccgtccgc
    13921 aacgctgccc gtccgcctgg caggccggct gaatcgccgg cctccgcagc gcgacgcaaa
    13981 ttcctcaacg gcatcgccgc cgctgcgggc ggcggctgcc tgctcgccct cggggccggg
    14041 ctctattccg gcagcgcgtc ggccctgcca gcccaggccc tgcgcccgcc cggcgccctg
    14101 gcggaggccg atttcctggc cgcctgcgtg cgctgcggcc tgtgcgtgcg cgactgcccc
    14161 tacggcaccc tgcgtctcgc cgatctgggc gaggcggtac cggtgggcac gcccttctac
    14221 accgcccgcc gcgtcgcctg cgagatgtgc gaggacatcc cctgcgtcaa ggcctgcccc
    14281 accggcgccc tcgaccacgg cctcaccgac atcaaccggg cccgcatggg cctcgccgcc
    14341 ctcgtggatc acgagagctg cctgaacttc ctcggtctgc gctgcgacgt gtgctaccgg
    14401 gtgtgccccg tcatcgacaa ggccatcacc ctcgaaaccc agcacaaccc gcgctccgac
    14461 cgtcacgcca tgctgctgcc gacggtccat tcggacgcct gcaccggctg cggcaagtgc
    14521 gagaaatcct gcgtcctgcc cgaagcggcc atcaaggtct tcccgcgtaa gctcgcccag
    14581 ggcgcgctgc ctgcccacta ccgcaagggc tgggaggaaa aggcccgcca cggcggctcc
    14641 ctgatcggcg accaggtgga actgccgatc cgcggcttcg aaggctccac tgccggccgc
    14701 gcgccgaccc agacggtcgt cgcacccacc gccccggccc ccgcgctgcc ggcgccgacc
    14761 gcggaccttc cccgcccacg caccccggcg ggcagcggca tcaactccca ctggaaaccc
    14821 tgagcatggc caccgccccg tcctgccagc tttccgcgag cccctccgcc gaccggcgca
    14881 gcctgtggca gcgccagcgc tggctcatcc tgcgacgcct cagtcaggcg tcgatcctgc
    14941 tcctcttcct ggccgggccg tggtttggcc tgtggctcgt caagggcaac ctgtcctcct
    15001 ccctcaccct ggacgtactg cccctcaccg atccactgct gttgctccag accctcgtca
    15061 ccggccacgt gccggaagcc accgccttcc tcggcgccgc cctggtcacc ggcttctacc
    15121 tgctggtggg cgggcgggtg ttctgcggct gggtgtgccc ggtcaatatc gtcaccgacg
    15181 ccgccgcgtg gacgcgccgc cggcttggca tcaagaccgg ccgcgtgccc gatgccgcca
    15241 cccgcctgtg gctgctagcc ggtgtgctgg gggtggccgc ggtaaccggc cgcctcgcct
    15301 gggagtgggt caatcccgtg tccctgctgc accgcagcct gatcttcgga ctcggcggcg
    15361 gcctgtggat gatggccgcc atcttccttt atgacgtgct ggtcgccagc cgcggctggt
    15421 gcggccacct gtgtccgatg ggcgccttct acggcctgct cggccagagt gccctggtgc
    15481 gcgtctcggc ccatcggcgc aaggcctgcg acgactgcat ggactgcttc gccgtgtgcc
    15541 cggaacccca ggtgatccgc ccggccctca agcaggccgg ccagacccac cccctcatcc
    15601 tgtccgcgga ctgcaccacc tgcggacgct gcatcgacgt ctgcgacaaa caggtattca
    15661 agatgaccca ccggttcgac caaaggagcg agtgatgaac acgccaaaaa tccaaaccct
    15721 ccgcctgcgg gccgctgccc tggccctgtg cctcgtccac gtcctgggcg tgggcatcct
    15781 cggcgctcct gcccaggccg ccgacgcccc ggtcaagctg cagagcctgc gcggcgccga
    15841 agtgccggca tccgacccgg aaggagacgg tgcccgctac ggccgcgacc aaccgcccat
    15901 gccccgcgac ttcgtccagc agccgcccct gatcccgcac accatccgcg gctacacgat
    15961 caccaagaac ttcaacaagt gcctggactg ccatgcctgg agccgtgcca aggagaccgg
    16021 cgccaccaag atctcggtga cccacttcaa ggaccgggag ggccaggagc tgtccagcgt
    16081 gtcgccgcgc cgttacttct gcagccagtg tcatgtgccc cagagcgatg ccaagcccct
    16141 ggtgggcaac accttccgcc cgggcgtggg catgcgctga tccatcatca ccgggagccc
    16201 tgacatgaac aatacgaccg gcctcatcgg ccgcctgcgc accaccggct ggggtcttgc
    16261 cgcgctgatc ttcatcgccg gcatcgtgtt ctggggcggc ttcaacaccg tcctcgaact
    16321 gaccaaccgg gaagccttct gcatctcctg ccacgagatg aaggacaacg tgtatcagga
    16381 ataccgcaac accattcacg cgtccaaccg ctccggcgta cgggccacct gcccggattg
    16441 ccacgtgccc aaggactgga tacccaagat catccgcaag atccaggcct ccaacgaagt
    16501 cctgcacaag gtgctcggct ccatcgacac acccgagaag ttcaacgcca agcgcctgga
    16561 gctggccaag catgagtggg agcggatgaa gaagacggat tcccgcgagt gccgcaactg
    16621 ccacaactac gacagcttcg actatgccga gcagggtcgt cgctcgtcgc ggatgcacca
    16681 gaccggcctc aacgaaggga agacctgcat cgactgccac aagggcatcg cccaccagct
    16741 tccccaggtg gaacagtcta tcggcgcgcc caaggagggt gccacgcccg aggaattcca
    16801 ccccgccccg gccgcacccg cgccggcgac ggcccccgcg gaaaagagct gacgcattcg
    16861 gtggagcagg ctccggtcgc ccccggccgg agcacagggg agacgacggg ctgctgtggc
    16921 agcccgtcgt ctttatggcc acgccatcgg tatgcggccg cccccaagac gtcgtactat
    16981 ctgcgccacg gccggacacg cagccctcaa gcccggcgtc accgggacgt tatagaaacc
    17041 cgtcagggcg acgggtcttt cgcgcccgca gccaaggtag cccatgtcgt cccccaatcc
    17101 acccggttct ccttccagcc gacgcctgca cggccccatc gtcgccctgg tcatcgccct
    17161 gatcgtgctg atctgggggg cgatcctgta caagatccat accgaagagg aactggtcat
    17221 ccactcggtc aacgtggaaa acctcaacct cgcccgtgcc tttgaggaac acaccatccg
    17281 caccctcaag agcgtcgatc aggcggtgct cttcctcaag ttccagtacg agaagtacgg
    17341 tgacaaggtc gacatcggcg agtacgtgcg ggaaggcatg atcatcagca ccatcttcaa
    17401 ccagctgggg gtcatcgacg aacacgggat ctacatcctc agcaacctcc ccaatcacaa
    17461 gaagatcgac ctctccgacc gggaacactt caaggtccac atggagcggg acaccaacca
    17521 gctcttcatc agcaagccgg tgctgggccg cgcctccaag aagtggtcga tccagatgac
    17581 gcggcggatc aacaagcccg acggcagctt cggcggcgtg gtggtgatct ccctcgatcc
    17641 gatgtacttc tccgatttct acaagggcgt ggacctgggc acccacggcg cagtcgccct
    17701 cgtgggcctg gacggtatcg tgcgcgcccg ccgctccggc aacaacgatt ccgtcggcca
    17761 ggacatcagc gccagcccct tcttcaccca gaccaacacg ggcacgacgg ggagcttccg
    17821 ttccaccagc atctccgacg gcatcgtgcg cctgtacagc taccgggccc tcaaggacta
    17881 tcccctggcc gtcatcgtcg gcgcggccga agacgaggcc ctgcaggaat tccgcggccg
    17941 ccgcgacggc tacctcgcct acgccgccgg catgagcctg ctggtgctgt tcttcggcgg
    18001 cgtggcgatc cggctcctca accgtcagac gcagatcagc gatgccctcg acgaggcccg
    18061 tgtccgcgcc gaatccgcca accgcatgaa gtccgagttc ctcgcctcca tgtcccacga
    18121 gctgcgcacg cccctcaacg gcatcatcgg ctacgccgaa tacctgcgtg acaccctcga
    18181 aggcccggac aaggaattcg cccagaccat ccacgagagc ggcgaacacc tgctcgaact
    18241 ggtcaattcc atcctcgatc tggcccgcgt cgaatccggc aagatgaccc tcgaattcca
    18301 ggaggagatg ccgcgcagcc tcatcgacaa ggtcgtccgg gcccagttgc ccaccgccga
    18361 ggccaaggga ctccagctcg agacccgatt cgctgacgat ctgcccgacc acatcgtgtg
    18421 cgacgggacc cgcgtggtgc aggtcctcaa caatctggtc aacaacgcca tcaaattcac
    18481 cgaccagggg caggtcaccg tcaccgccgg gcgcgacggc aagatgatgc gggtcgccgt
    18541 caaggacacc ggttgcggca ttcccgccga catgcaggac tccatcttcg agcgcttccg
    18601 tcaggtcgaa ggcttcctca cccgccgtca gcagggcagc ggcctcggtc tggccctggt
    18661 caaggaactc ctcaccctca tgggcggcag cgtcaccctc gaatccaccc ctggccaggg
    18721 cagcgaattc atcatccgca ttcccttcac gcccgcccgc cccgatcctc taggacatgc
    18781 ctcatgaaag tcctcgtcgt cgacgaccaa cgcgtcaatc gcaccctgcc cgccgtgctc
    18841 ctgcgccagc aggggtgcga ggtcctcgaa gccgaatccg gcgaagacgc cctccggctc
    18901 atcgacagcc acggtttcca ggccatcctg ctggacgtga gcatgcccgg catcagcggc
    18961 accgaactgt gccgacagct gcgcagccgc cctgaattcg gcggcgtacg catcatcgcc
    19021 tacaccgccc acgccatgcc tggcgaacgg gaagacatcc tctccgcggg tttcgacgac
    19081 atcctcatca agcccatcaa ccgccaggtc cttctggccg ccctgcgcct gcccgcctga
    19141 gcaggccgat ttcccccctg cggcggctgc ggctgccgct acaatgccgc tcccttcgac
    19201 tcagtacgga tcgatttgcc catgtggttc cgcaacctcc agctctaccg cctgcccgaa
    19261 aactgggacc tcagcgccga caccctcgcc gacaagctcg ccgcccgccc cttccagccc
    19321 tgtggcagcc aggacatggc gagccgcggg tggacctcgc cgacccgcga cgaccgcttc
    19381 gtcctcgccg tgggcggcca ctacctgatc gccctggccg tggagcagaa gctgctgccg
    19441 tcctcggtgg tcaaccaggt ggcggccgac aaggccgagc tgatcgaagc cgaacagggc
    19501 tacaagcccg gtcgcaagca gatgaaggag atcaaggagg cggtgctgca ggaactcctc
    19561 cctcgcgcct tcacgcggcg gcgcacgatg tgggcgtgga tcgacccgaa gggcggctgg
    19621 ctcgccgtgg atgccgccag cccggcccgc gccgaggacg tcctcgacgc cctgcgtgac
    19681 accctcgacg ccctgccggt gaagctgctc aagacccagc agtcgcctgt cagcgccatg
    19741 accggctggc tggccggcgg cgaggcgccc ggcgccttca ccatcgacca ggattgcgaa
    19801 ctgcgcgccg tcaccgacga gaaggccgcc gtgcgctacg tgcgccacgc cctcgacggc
    19861 aaggacgtgc aggaccacct ggccgccggc aagctgccca cccgcctcgc cctcaccttc
    19921 gacgaccgca tcgccctgct cctcaccgaa aagctggaga tcaagcgcat cgccttcctc
    19981 gacgtggtca aggaagaggc cgaacgtcag gccgaaaccg ccgacctgca gatggaagcg
    20041 gatttcgccc tgatgaccgg cgaactctcg cgcctcatcc cggccctgct ggaggccctc
    20101 ggcggcgagc agaaggacgc gttctgacgg ccacggcgga cgccgcgaag ccgtccgcca
    20161 tcgcgccgcg ccggcctcag atgccggcca gcgccagccc ggcgatggcg catccggccg
    20221 tcgccacccc gaccgaggtc cattgcatcc agcgccgccg cgtcagcagc gtgatcgccg
    20281 ccagggagat cgccacctgc atcgccgtca tggcctgggc ccagcggtga tgggcgtgca
    20341 tggcatgttc gctggcttcc tcggcctcga tggcggcttt ttccagttcc tccgccgcgc
    20401 tcttgatctc ctgcttctcc tgcttgtagc gctcgatctc ggccgtgtag cgggtctgga
    20461 tttcccccgt cgtaaggctc acgcccagct ccgccagatt ctgcttggcg ctcttggcct
    20521 ggtaatagcc ccaccggttg gacgcctccg tcttgcggat ggccgcctcg ttcttgtgca
    20581 tcagcgcgtc gttctgggtg gcgccaccgg catagctgaa cagcgcaccc agggtggcga
    20641 ggatcgccgt catcgccgcg atgcgcgacg cgaaggggtc gcccccgcca tgttccgcgg
    20701 catgctcgac cgcgtggtcg tgggggccat ggacgtgaaa atgatcgccc gacatgtgca
    20761 actcctgaaa tcaatgggat gggccggcgg acgccggccc ggcgggcggc attctaggac
    20821 agggatgtga ccgcgcaaac caccgcacac gcccatgcag gccgcagcgt gcggcacgac
    20881 gccccgggcg ggcaccctca gggcgtttcg cgcttcagga atgcttgcgc gtgcgccgct
    20941 cgaggagggc gatctcttcc ttgtagtggc ggatctgctc gtggatcttt gcctgttcgc
    21001 tcaggggcag gatgcccgtg gtcatgagct tgtcctccag ggcgtggatc ttgccgatga
    21061 gatccttgat ccgttcgaat ggcgtcggaa gcatggcggg ggcctgttgt tgtgcgtgag
    21121 ggatgtctcc gccctcatct tagccccgga accggccggg atgaaggccg cacgccggct
    21181 cagggcagga acaccggatc cgagaacacc agcgtcccgt cgggacgcat catcaggttg
    21241 tggggattga ggatgtccgg ctgcaccgga tagccttcga tgaaatccga caggtccgcc
    21301 accgccttga ccaggctcgt cggcagaccc ggcggcggtt cctgggcgat atggtaaagc
    21361 gccagacggc cccgattgac acccaggttg gcccacttct cgcagccctg ccaataggca
    21421 tcggagagca gctgcccgag cgcctcggcc tcgcccgcca ccggcagcag acgctccagt
    21481 tcgagcaggc ggaaggaata gccgttgctc gccttgccca ccacgccgtg atccttgatc
    21541 aggcgcggga agtgcaggcc gatcgggcga tcatcggccg cgaggaaaca atagtcggcc
    21601 ggggaactga ccaccttgaa gacccgctcc ccgtcgccgg cgtccagcac gatggacgac
    21661 tccccccgcc cgagctcggc cagcccggcg agcagggggt ggtcgggcac cggatcggcg
    21721 atgcggcggt cgtagctggc cgcatcgcga tcaggtcgga cagccatcgg cgggctcagg
    21781 cgatgatgtt gtagccggca tccacgaaat gcacaccgcc gctcaggttg cgacccgcgt
    21841 ccgagaccag gaaggcggtg agagcgccga tgtcctccgg cgtcaccaga cggtgcagcg
    21901 gcgaacgctc gactgcggcg gccatcagcc cgtcaaagcc ggcaatcccg gacgccgcac
    21961 gggtcatcag cgggcccggg gagatggcgt tgacgcggat gttcttgggg cccaactcga
    22021 aggccatgta gcgcgttgcg gcttccagcg cagccttgca caggcccatc acgccgtaat
    22081 tggcgatgac cttttcgctg cccaggtagg tcatggtgat cagcgaaccg ccgccggcct
    22141 tctcgaacag gggctcggcc ttgcgggcca ggcgcacgaa ggagtgggtg gacacgtcca
    22201 tggccagggc gaagccgtcc ggcgaggtgt ccacgacgcg gccatgcagg tcgtcacgct
    22261 tggcgaaggc catggagtgg atggcgaagt ccagcttgcc gtgcttggcg tcgatctggg
    22321 cgaacacgtc gtccatggtc tcgggcttgg tcacgtcgag caggtagacg tcctcaatac
    22381 ccagacgcgc gacgatgggc tcaatgaagg ccagggtctt gtcgttctgg taggtgatga
    22441 ccacctttgc cccggcctcg atgcacgctt ccgcgacgcc ggtggagatg gatttctcat
    22501 tggcgatgcc ggtgatcagg cccactttgc ctgccagatt gatgatcggt tgcatgacgg
    22561 actctcctag cttgcgtgaa aaaatactgc attgcagcaa aacctgaccg tagaatagtc
    22621 cagattgctt gctatggttg agttatcggc aagagatgtc agagtcccac cccttttttg
    22681 aacggatatt gcgatgcatc aaaccgaggt cagtccgccc gcagccgccg gccccggtcc
    22741 ggtggtcgtg ttcctggatt tcgacggggt cacccatccc tggggcgaat ccgaggattt
    22801 ccgctgtctg ccggtgttcg aggcagtgtt gcgggactac ccggaggtcc gcgtggtgat
    22861 cgcgtccgac tggcgcacgc tgttctccct gcccaagctg acggcgcgct ttgccgaaga
    22921 tctgcgggcg cgggtggtgg acgtgtcgcc caccatcttc cccaaggaag ccaacgagct
    22981 ctacggcctg cgcgaacggg aagcccgcca gtggctggcg cgccacccgg acatcgccgg
    23041 ctggctggcg gtggacgacg cgccgggcaa ctggccgacc cgcagccgcc tggtgctcac
    23101 cgatttcaag cgcggcttca ccgaagagga cgccggccgc atgcggcgca tgcttgacgg
    23161 cttccgccgg ggagaatacc cggagcaggc cgccgcctga gccctgccgc cgcgcggccg
    23221 cccgtacgtg aagcgcgcca gaacggccgc ttcgacacgg aaccgcgtca gaactgccgc
    23281 gtcagcggcg attcgtccat cagcgccacc tgctcccgca gggccaggat catgtcctgc
    23341 caatagcgcg ggctgtcaaa ccacccgaag gcggccggaa aggccggatc gtcccagcgg
    23401 cgggcaatcc acgccgcgta gtggatgagg cgcagggtgc gcagggcctc caccagatgc
    23461 aggctgcgcg gatcgaagtc ggcgaaggcc tcgtagcctt ccagcacgtg atccagctgc
    23521 cgcgccatgg ccggcgcgtc gccggacagg agcatccaca ggtcctggat cgccgggccg
    23581 ttgcgcgcat cgtcgaaatc cacgaagtgc ggtccggcgt cggtccacag cacgttgcca
    23641 ccgtggcagt cgccgtgcag gcggatcgtc ggcagttccc ccgcacgcgc gtaacaggcg
    23701 tgcacgcccg ccagggcctg atccaccacc gactgccagg tggcacgcag ctcggccgga
    23761 atgaaggcgg tttcaagcag cgcgtcgcgc gcctcctcgc cgaagctgcg gggcgtcagg
    23821 tccggtcggt ggacgaaggg acgggtcgcg ccgaccgcgt ggatgcgccc caggaagcgc
    23881 ccgagccagg tgagcacggc cggatcgtcc agctccggcg cgcgcccgcc acggcgcgga
    23941 aacagcgcga agcgaaagcc ctcgaaggcg tgcagggtgc gcccgtccag ttccagcgcc
    24001 ggcacggcgg ggatctccgc cgccgccagc tcggccagga aggtgtgttc ctccaggatc
    24061 gccgcgtcgc tccagcgccc cggccggtag aacttggcca ccaggggtgc cgcgtcctcg
    24121 atgcccacct ggtagacccg gttctcgtag ctgttgagcg tgagcagacg cccgtcacag
    24181 aagaggccgg cggattccac cgccgccagc acgaaatccg gcgtgagcgg cccgaagggc
    24241 gccggcgcgg cggcgccgtc cccggcgctc acgcgtcgcg gcccggttcg cgcacgtagg
    24301 gttcgtgcag cttcaccggc tcggccgcct ggcggctgcg catgcgcagg ttgagcatct
    24361 cgaccatcac cgagaaggcc atcgccgagt agatgtagcc cttgggcacg tgctggccga
    24421 aggcgtcggc gatcagcacc acgccgatca ccagcagaaa ggacagggcc agcatcttca
    24481 cggtgggatg gcgcgacacg aagtcgccga tggggccgga ggccaccatc atcaggccca
    24541 ccgaggcgat caccgcggcg atcatcacgg tgaggttgtt caccatgccg atggcggtga
    24601 tgatggaatc cagcgagaac acgatgtcga tcaggacgat ctgcagcagc accgcgccaa
    24661 aggtggcctg aacggtactg gaggcctcgc cttcctcgcc ttccatgagc tggtggacct
    24721 ccatcgtgct cttccacacc aggaacagac cgcctcccag caggatcagg tcacgccccg
    24781 aaaagccatg ccccagcacc gtcagcaggg gttcggtgag ccccgccagc caggacagga
    24841 tcatcagcag gccgatgcgc atgaacatgg ccaggaacag ccccaggcgc cgggtgaagg
    24901 cgcgccgttc cggcggcagc ttgtccacca ggatggagat gaagatgacg ttgtcgatgc
    24961 ccagcaccag ctccagcgcg gtgagggtca gaaaggcgat cagcaattgc gggtcggtga
    25021 ggaattccat ggcggctcgc ggcagttgtc cggatgcggc ccgcacggga gccgcggcgc
    25081 gcatggtcgc atgaatccgt gacgggcggt cggccgtccg gcggacgctg cggccggacc
    25141 gccggagccg cctggcaggc ggcacgggcg acgggatcag tgctgcgtgc ggtgttcctg
    25201 catccagcgc tgcatgcgcg ccgccggcat cggacgggcc acgccatggc cctggatcat
    25261 gcggcagccc aggcgcagca gggcctgacg gtgacccggc gtctccacgc cctcggcgat
    25321 cacctcccgg tggaaggctg cgccgaggcc gatcacgctc tgcaccgtgg ccaggtcttc
    25381 ctcgtcctcc agcatgttgt ggatgaagga gcggtcgatc ttgatgctgc gcaccggcag
    25441 ccggcgcagg ctggacaggg cgccctggcc ggtgccgaaa tcgtcgatgg cgaagctcac
    25501 gcccagttcg ttgcacgccg ccatgatgcc ggccacccgc ttcacgtcgc cgagggtcgc
    25561 cgtttccagc acctccagct ggagcagctc cgggtgcacg gccggatagg ccgccagcag
    25621 acggcgcagg ttggcgacga actccgggtg caccagatgc cgggcggcga tattgacgct
    25681 cattggcgtg ctcaggcctt cccggtgcca cgcgtcgagc tgcttcactg cggtacgcag
    25741 cacccagtcg cccagttcga tctcgaaacg ggaatccttc accgccggca ggaactcgcc
    25801 gggcatgcgc agacccagct ccgggtgctt ccagcggatc agcgcctcca cgccggtgag
    25861 ggcgttggtg agcacatcca ccttgggctg gtagtgcagc tccagttcgc ccccgtcgag
    25921 ggccagctcg acccggtgct gggcgtcgcg cagggcgcgc aggcgccggt cgcggcccgg
    25981 atcgtagagc tggaaggcgt tgcggccgga ttccttggcc aggtacatgg cctcgtcagc
    26041 gtgcagcagc aggtgttccg gatcggagcc gtccagcggg cagagggtca cgcccacgct
    26101 ggcggtgacg gtcagctccc ggttgtcgat ggtcaccggc tcgccgatgc tggcgagcag
    26161 gcgttgcagg atctgctcgc actcctcgct gcgctcgagc ccgttgtaga tcagcgcgaa
    26221 ctcgtcgccg ccgatgcgcg ccacggtgtc gccggcccgc gtggaggcca tcagacggcg
    26281 ggcgatgacg accagcaggc ggtcgcccac ggcgtggccg agggaatcat tcaccgcctt
    26341 gaagccgtcc aggtccaggt atgcgatggc cgccagggtg ccattgcgct cggcctgggc
    26401 gacggccatc tgcatgcgat cgtagaacag gctgcggttg ggcagctggg tgagggtgtc
    26461 gtggtaggcc atgtgctcca ggtgacgctg gctttccttc acttcggtga tgtcagcgat
    26521 gctgcccatc atgcgcgagg gctggccgtc ggcgtcgaat tccaccacgt cgccccgatc
    26581 gcgcacccac agcacccgtc cgtcggggcg acgcatgcgg tactcgcaga tgtaggcccc
    26641 gtgctgggcc atgcaggcgg ccacggcggc gttatagagg ggccggtcgg cctcgtggat
    26701 catcgccagc aggtcgtcca ccgaatggat cagctgcgcg tggccgagtc ccagcagttc
    26761 gcaccagcgc cggttgtgac gcaccgtgcc gctggccaga tctcggtccc acacgccttc
    26821 accgatggct gacagcacga agttgaggtc cttctcgctg cgctcggcgg cggccagcag
    26881 acggatgcgc tcactcacat ccacagcaaa gcccaccgtg ccgacgatgc cgccttcagc
    26941 gccgcgcagg ggcgcccgca cgctctccag ccacaccggc gaggctccgc cgctcacctg
    27001 gtccaccgcc cgcatggcct cgccgcccgc cagcacctcg gcgtcggctg cggcatgcag
    27061 cgccgcgcgc ccggcgggcc acagctcgtc gtcggcccgc ccgatcacct cggccgatgt
    27121 actccgtccg caggcttcgg caaagctgcg attgacgatc agatagcggc cttcggcgtc
    27181 cttcatccac aggagcaggg gcaaggcgtc gatcagggtc ttcgccgaac cggctgcggt
    27241 ggcgctgtcg gccgaggtgt ccaggttcag acgcgacacc ggcgtgtcgc cggacaggcc
    27301 ggcatggagg gcgccgagca gggtggcggc ccccaggtag cggcgcgccg gcccggcgtc
    27361 acccaccggc aggtacgacg gtcccccggc gagaaagagg gacagggcct cgaagagggt
    27421 ggcgtcgggc gacagggcat cctctcggga ggcgccaggc ccggtctggg aatcggcagc
    27481 ggacttcatg ggcagtgtcc atcgaacgtt atgggttgcc ccgatgatgc cccgcggcca
    27541 acgcttcgct aaggaaggat gtcccccccg cgacgggcag aaagtcacgg atccgccggg
    27601 cctcagcgac aaaattcacg ccaccgccgc gatttccgca tgtcattcac aggacttcac
    27661 agatcggcgc aggacgccgc tcccgcggat gccgcaccga cctggtgcag ggccggcggg
    27721 gtagaatgca gtgccggcac cccgccggaa cgtggagtcc cccgatgagc cccgacaaga
    27781 tcgacattcc cggcctcctg agccgcatgc ccatgttcag cgccctgtcg ccggacgatc
    27841 tcgcccacat cgctgcggcc acccgcgagc gtcgcctcga caagggcgaa cgcctgttcc
    27901 agcgcggcga cgcgccaaag ggcttcttcc tggtggtgtg gggccaggtc aagctggcct
    27961 ttacctcttc ccagggcaac gagaaggtgg tggagatcct cgggccgctg cagagcttcg
    28021 gcgaggccgt gatgttcctc gaccagccct atccggtgct cgccgaggcc ctggccgacg
    28081 ccttgatcct gcatatcggc tcggccgccg tgttcggcct catcgacgcc gatccgaaat
    28141 tcgcccgcaa gctgctcgcc ggcctgtcga tccgcctgca ctccatggtg cgggacgtgg
    28201 aatcctatgc cctgcgctcc tccacccagc gggtcatcgg ctacctgctg cagctggccg
    28261 aaagcacgcc ctgcgccgcc cagggcgaac ggagcgtcga gctgcccacc agcaagcagg
    28321 tcatcgcctc acgcctgagc ctgacgccgg aaaccctgtc gcgcatcttc cacgagctgt
    28381 ccggcgccgg tctcatctcg gtgcacggca agcagatcgt catccacgac gccgaacgcc
    28441 tcgccgcgca cgactgacgc ggacagatca gtccgtgcgg tggcggatgt ggatggcgaa
    28501 ctcgccgccg ggcgtggatt cggcgcgata gacgtagccg ttgcggatca gcacgtcgta
    28561 gaggggcgtc ggttcccggt agagcaagag gatcacctcg ccgccggcgg gcagggtgtc
    28621 gagggcttcc agggtcagat gcatgggctg gggcggcatc aggccgcggg catcgacgat
    28681 cacgggggca agggtttcgc tcatgggggc gctcgcagga aggacccgtg cagcttgggc
    28741 gcgggcgcgt cgcgggtcat tgagccgcgt caaggcgctc cgatccggcg gacgctccgc
    28801 cgggcccggc gcagggatgc tacagttccg ccccgatccg accatccgcc accgccatga
    28861 aggaaggcac cgtcctgctt gtgtcctgtg ccgccaccgc cgcgctctgg ctgtgggcgg
    28921 agcagaaccg cccggcaccc gccctcccgg aggccgcgcc gacaccgtcc gcggaggcgc
    28981 ccccgctcgc cagcccgccg gtcgccccgc catcgccgga cagccgcgcc tacctgtgca
    29041 ttgccaacgg ccagcgcagc tattccgacc gcccctgcgc cgccggcgag cgctccgagc
    29101 gcgtgaatat ccaggcgccg ccgcccggct caccgacccc cagcatccag gagcaatacg
    29161 ccgatctggt cgcgaaccgc cccagggtga cgcccggcgc cgccgagccg ctggtcatcc
    29221 cacgcgatcc cgatcacccg gagaaagccg gcctgtgtga agagctctgg ctgcacatcc
    29281 gggagatcga cgaggccctg cgccagccca cgccacccca gcaggccgag acgctgcgcg
    29341 ccctgcgcct gcgctaccag aacgatcgct tcacccaggg ctgttaaccg ccgccgcctg
    29401 gcggactgcg gacgccggat cgtccccccg cgagccgtcg cggcctcggc gatgcgggac
    29461 cgcacccact accgtggccg ccggtcgcag ccgcgcccgc tgctccgggt ggcacctcag
    29521 tggctggtgc cttccgattt tccgatcttg ttgaacaggt tgtacttgcc gatgggcttg
    29581 ctcgccggca ggcgcttgat ctcctggaag gtggtggcgt cgaggaagat cagcgcgccg
    29641 tcgttctcca tcaggctggc gatgacgtag cgcccgtggc ggtcgaactc cacgtgggcg
    29701 aaggtccgac ccggctcggg ccgcacctgg gccaccaccg ccaggcgctg cttgtcgatg
    29761 acctgcaggg tgtccttggc ggtggggctc atcatcgaat cgacccacgc gtaggggctg
    29821 ttctcgtggc tgcgcaggaa gaagccgggc cccagggtgg gaatcgtcgc cacggtctgc
    29881 cagcgctgca ggtcgatcac actgacgcgc ccctccttca ggtgggtgct ggccaccacc
    29941 cggcggccgt cgcgcatcca cgagatgccc gagcccaggt ggggcatgcc gggcaggtcc
    30001 agggtggcga tggcgcggcg cacgtccagg ttgaccacct cgccgcggcc ttcgcggctg
    30061 gcgcccacca cgttggcgta gtcctgggtg aagaagaagt cgtccagggg cgtgtccagc
    30121 gccgtgcgac gggaattgag gaagcctggc agggccatcg cctcgcccat cttgtagtcg
    30181 tgcacgtagc catcgaagat gggcttggcg tccgggtcgt aggacacttc ccagatctcc
    30241 gccacatcct tgaggccgac gatgaagctg cgccgcggtg gtgcgtcgta caccgccgcc
    30301 acgcgggagc tcgcgtcgcc tttcagcgtg cgcgccggga tcaggcgcag cagcttcagg
    30361 tccgcgtcga agatcgccac gttgccgggc aggtagttcg ccaccgccac gaagcgcccg
    30421 tccgccgaca ccgcggcgtt gcgggtgttg agcccggcgc gcacctcggc caccaccttc
    30481 aggttccaca ggtcgaactt gctcacccag ccgtcacggg aggcgaagaa cacgtagcgc
    30541 ccgtccggcg tgaacttggg cccgccatgc agggcatagc gggacggaaa acggtggatg
    30601 cgcgtcatgc ggtcgccgtc gagcacgctc acgtggtgat cgccggcctc cacgacgatg
    30661 aacaggttca tcggatcggt ctgggccacc gggccctggg gcgcatccgg caggctgccg
    30721 cgggggaagg gctcgacgcg ggaggcgcgg atgtcgtcct cgctccaggc gggcgccgga
    30781 ctcaccggcg tgcgcaccca gtccgccagg gcctcgatct ccgccgccga gagggtcgcc
    30841 ccgaaggcgg gcatctgggt cgcggtgcgg ccgtcgcgaa gggtggcgat cgcgtcggcg
    30901 cggcgcaggc gttccaggct ctccggcagc agggccggac ccatggcacc gaggcggccc
    30961 ggcccgtgac agctggcgca gtgctgggtg tacagggccg gtgcgtcgac cacggcggcc
    31021 gcaacggtgg gcaagagcgt cgccgcaaag gcgaggctgg cgagcagtct tttcattcca
    31081 tccctcccgg tgcccagggc cgttccggta cggggtcttg atcgtgtccg ccccgcgggc
    31141 gaagggcgcg cacccgcgcg caagtgagca gtcctcgcac gtgggtgaat ttcactggcg
    31201 ggcgcgtgaa cgccggtcgc gcgcgaatgg acacgggtcg cggacgaccg gccggcactc
    31261 gccggtgggt attggccact cgtccgcgag taaaaatcac tcgcgcgcgg gtgaagagca
    31321 ttcgtctgcg agtgacggcc cctcgcgggc gaatggggcg cagtcgtcgg cgaggaacgg
    31381 tcacccgcgc gccggtgaac ggcgctcgcg agaggctgaa gcccatccac cggagcggaa
    31441 aagtcatggg cccttgcgcc aggcggatca ggccggctgc agacgcaccc acggcttcac
    31501 ctccagacgc tcgccatcgg ccacgccgat ctcggcgttg tccaggtagc agccgggatc
    31561 ttcctgccag ggatcgccgg tgagctgcca ggcgcggacc cgggtgttgc cattgcacat
    31621 ggccaggtaa cggcaggccc cgcagcgccc gctgacgggc cgcggcgact gcttgaggcc
    31681 ggccagcagg gggtcggaga g
//
LOCUS       DAOQMM010000031        31344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_386_length_31344_cov_45.352808, whole genome shotgun sequence.
ACCESSION   DAOQMM010000031 DAOQMM010000000
VERSION     DAOQMM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 31344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31344
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_386_length_31344_cov_45.352808"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLH85_07400"
     CDS             complement(<1..182)
                     /locus_tag="PLH85_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNB64424.1"
                     /translation="MAAKEVKFGDSARARMVEGVNILADAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEI"
     gene            complement(234..524)
                     /gene="groES"
                     /locus_tag="PLH85_07405"
     CDS             complement(234..524)
                     /gene="groES"
                     /locus_tag="PLH85_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNB64425.1"
                     /translation="MKIRPLHDRVIVKRIEAERTTSSGIVIPDSAGEKPDQGEVLAVG
                     NGKVLDNGEFRKLEVKVGDRVLFGKYAGQTVKVDGQELLVMREEDIMGVVEA"
     gene            1037..1504
                     /locus_tag="PLH85_07410"
     CDS             1037..1504
                     /locus_tag="PLH85_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB64426.1"
                     /translation="MTTPPSALRRQLTTLLIRAARLYRKEVNRALTTYGISDAQAIPV
                     LHIARHGGGMRQHELAEEIGIEGPSLVRLLDQLCAHGLVERRSDPQDRRARTLHLTDA
                     GEALAARVETALVGIRGRLLAAVSEADLSTCLRVLESLLHQLDVGPSHHDDPA"
     gene            1470..3539
                     /locus_tag="PLH85_07415"
     CDS             1470..3539
                     /locus_tag="PLH85_07415"
                     /inference="COORDINATES: protein motif:HMM:NF016513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNB64427.1"
                     /translation="MSGHPTTMIRPEPLLRLDWRDVQFSVVSLAAALAALYWSMSLDL
                     QQPYWSMLSAYVVSQPLAAAVRSKAVYRLAGTLLGAAMTVLLLPALANTPELLCAAMA
                     LWVGACLSISLLDRSPRSYVLMLAGYTAAIVGFSSVNQPGQIFDLAVARAEEISIGIL
                     CATLAHSLWFPRSVGEVLEQRIRDWLGEADRWALDLFAADDPSAGVPHRAHLAAAATD
                     IHLLAAHLPFDTSHLRETTAIVRALHDHILLLIPVLSSLSDRLAALHGMRAPVDPPTQ
                     DRLTRVGAWIRADCPAAEGRALSAMLAADLDTPAAAPDAASWYALNRNGLTTRLLDLV
                     QIQTECRQLLAHLHEPDAPLAPALRDALGQAGARPLHSDWRLALTSGAVATTAILICC
                     AAWIGLGWSEGGASAMLAAILCCLFASLDDPAPAIRKFGVAICIAVSLAALYQFAVFP
                     AINTFPLLALVLAPPLLAIGAATLNPRLASSALFILLNFCNFMAIQERFTPDLAVFLN
                     NNLSQFFGVFVAIYVTRAARSLSTASSARRLARATWQDLARFAHGDVREPHQNLTARL
                     VDRIGLLAPRLAALRDQDAELADGDVLRELRIGMDLSTLQDAHPPLPGIAQTQVTELL
                     HAVGRHYQQRPDGTARPPQDLCALLDGCIRHLAGPPGQGRALAALVGLRRNLFPDTPY
                     AAGGLNP"
     gene            3536..3745
                     /locus_tag="PLH85_07420"
     CDS             3536..3745
                     /locus_tag="PLH85_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HNB64428.1"
                     /translation="MNGEIELFGVYLSAELSTSIAALLLTFGLQRGLVWLGLLRRVWH
                     RPLFETALFIIVWRLVVAAWALHSP"
     gene            3769..4665
                     /locus_tag="PLH85_07425"
     CDS             3769..4665
                     /locus_tag="PLH85_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNB64429.1"
                     /translation="MNTTFLSKAAPVLLTLAIVAVALIAGRQLWIHYRSEPWTPDGRV
                     RADIVQIAPDVSGTVTDLAVGHDQPVKAGQFLFQLDPERYALALHDAESAERVQEARL
                     AQLQREVRRNQTLGELVSREAAEQTQAHLDEARAALAQARAQRELAALNLKRTRIVAP
                     ADGTLSDLTLRVGNYVAAGKPVMALIDSRSLRVEGYFEETKLPRINVGQTARVHLMGD
                     DRILTGHVQSIAAGIEDRDRTSGSSLLPNVNPTFSWVRLAQRIPVRIALDAPHDPALI
                     AGRTATVTLLPPTAERDGGPAR"
     gene            4662..6104
                     /locus_tag="PLH85_07430"
     CDS             4662..6104
                     /locus_tag="PLH85_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNB64430.1"
                     /translation="MTPRAALVSGAIALLLSACSAVGPDYRLPDAAQLRSPDARQAFQ
                     EAGQPPFGRDDLPDAWWRLYEDPALNDLIARALVANAGLRAAAANLARSEAVVREVDG
                     LRDPSLGAGVAIERAQVSAESYLLEHKVPVFNLGNAGLRAHYQVDLAGQLRRALEVAE
                     AEAGAVQAAGDLARISVVADTALAYMDACAAGEALDLARRALALQVREREAIARLVAG
                     GRRIPLDLPRAAEQEDQLRAALPAFAARRRSAAYRLAALTGQSPARAPAAAEACRHLP
                     DLAQPIPVGDGAALLRRRPDVRQAERRLAAASARIGVATAALYPSLSFGLSAGGTGIL
                     SHLGEAPTQRWSLGPVLSWSLPGEEARARVAQADAGAAGALATLDQVVLNALQETESA
                     LATLARDRDRHAALQQARAHAETARRQVHTLYQAGRTGVLDDLDAERRLLAADARLAA
                     HRGRLATDEIRLFLALGGGWQPGEVHTTPQ"
     gene            complement(6176..7564)
                     /locus_tag="PLH85_07435"
     CDS             complement(6176..7564)
                     /locus_tag="PLH85_07435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HNB64431.1"
                     /translation="MGLNRRLSLTVRLTLLFALASSAVLLILGFIVGASVERHFEELD
                     RATLYGQLADLRRGLALGHLHGEAGGEGGLVEGAHLHPPMMELRGPDGRVLAASPGAV
                     FPSDVVIGAPGPQVWQDASGHWFRGLAAELPAAPAGAGPLRARVATDISHHQAFMDGF
                     RRTLWGVVVASALATGLLGWAAARSGLAPLEAMRRTAAQVSAQRLDPRLAVDSVPVEL
                     AALAESLNAMLARLEESFRRLSDFSSDLAHELRTPVSNLMTQTQVALSRPRSAAEYRE
                     VLESGAEELERLTRTIADMLFLAKADNGLIVPAREPVELADEVRALFEFYAALAEEVG
                     VSLSQAGAGRVAGDRLMLRRALGNLLSNALRHTPAGGAVRVTLGNAEDRVVVAVANDG
                     EPIPAEHLPRLFDRFYRADPARRHGSDGDGAGLGLAITRSIVRAHGGEVVVRSGVHGT
                     VFQMRLPPAGGS"
     gene            complement(7555..8238)
                     /locus_tag="PLH85_07440"
     CDS             complement(7555..8238)
                     /locus_tag="PLH85_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HNB64432.1"
                     /translation="MKILIVEDESKTGDYLRQGLVEAGFVADWVQDGLDGLHRGLTEA
                     YDLVILDVMLPGADGWQVLQGLRRAGKDLPVLFLTARDQVDDRVKGLELGADDYLVKP
                     FAFSELLARVRTLLRRGARAREPDVLKVADLELDRPRRRVTRAGRRIDLTAKEFALLE
                     LLLRRRGEVLPRSLIASQVWDMNFDSDTNVIEVAVRRLRAKVDEGFTPRLIRTVRGMG
                     YVLEETPWD"
     gene            8402..8725
                     /locus_tag="PLH85_07445"
     CDS             8402..8725
                     /locus_tag="PLH85_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HNB64433.1"
                     /translation="MRLRLLPAAPLAAVLLAALPAIAAEPTAHAAHATAAQALTDGVV
                     KKIDKAAGRVTLTHGPLNGMPGMTMAFAVKDPAWLDTLKPGERIRFAADTVNGALTVV
                     KIERP"
     gene            8739..10010
                     /locus_tag="PLH85_07450"
     CDS             8739..10010
                     /locus_tag="PLH85_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB64434.1"
                     /translation="MKPPRLVHLLLIGCAVALPAAACAQEAPLGRDVEGLLAWARAHS
                     PELAAMRHEADAAAERVVPAGALPDPRFRTELRDITRMGEQSPALLPARTGSTRYLLM
                     QDLPWAGKRDLKQAAAEQDARSASGRADATWADLSARIKITWTQLHYVHQSTRLTREL
                     LDLGGRIGQIAQVRYAGGLAPQQDAIRAQVELTGLQADLVALDNEGRQLQVRLNALLA
                     RPSAAPLAAAERLRPLPVPARLDPAALEDRLRARNPQLFADEARIAAAERNQALTLRN
                     RYPDFTVGISPIQYQNAVREWELMVELNLPLQQASRRAQEREADSLLAAARARREATA
                     QQALGELGEALSALDAARRTEALASQSLLPQAELTFRAALAGYETGKVDFTTLLDAQR
                     QIRLARQNRLKAQTEAQLRLADIERLVGEDL"
     gene            10007..11548
                     /locus_tag="PLH85_07455"
     CDS             10007..11548
                     /locus_tag="PLH85_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB64435.1"
                     /translation="MSAPRPALRLAAALLALAAAAGAGYGLGQRHGTEPPAASPTPAA
                     PAARKLLYYRNPMGLPDTSPVPKKDPMGMDYLPVYEGEADAEAAPGQVRLSPEKIQKL
                     GVRTEAVTLRALDRSVDASGRVEPDERRVVAIAPKFEGFVERLHVSVTGQPVARGQAL
                     FEVYSPELVSAQREYALAAQGADSLKDAGPEAQKGLRQLADAALQRLRNWDIPDAQIR
                     ALTHSGEARRTLTFTSPAAGIVTEKKAVQGMRFMPGETLYQVTDLSAVWVVADVNEQD
                     IGAVRPGSPVKLRVDAYPGETFDGRVSYIYPTLNADTRSVPVRIELANPSLRLKPAMF
                     VHVSLPAGSAAPVLTVPSAAVLDSGTRRVVLVERGEGRFEPREVRLGARSARYVEVLD
                     GVKADERVVVAANFLIDAESNLQAALGAFTATGGTPPAAQAASVVHRAEGQVDGADAA
                     AGTVTLSHGPVASLNWPAMTMDFAVANPALQAALKPGARVAVDFVERKPGEWVITAVR
                     PAAGR"
     gene            11559..14687
                     /locus_tag="PLH85_07460"
     CDS             11559..14687
                     /locus_tag="PLH85_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB64436.1"
                     /translation="MLAALIDWSGRNRFLVLLATLCVVLAGVYAVLRTPIDALPDLSD
                     VQVIVYTEFPGQAPQVVEDQITYPLTTAMLSVPKSRVVRGFSFFGVSFVYVIFEDGTD
                     LYWARSRVLEYLSAAAGRLPAGIMPQLGPDASGVGWVYQYAVLARDKTLAELRSLQDW
                     TLRYPLAKARGVAEVASVGGFVQTYQVTVDPVKLRAYGIPLSRVAQVIRASNRDVGGR
                     VVEMAETEYMVRGRGYLRGVDDIASLVVKAERGTPVLVRDIGRVELVPDERRGLAELD
                     GEGEAVGGITVARSGQNALDVIRHVKATLADLAPGLPDGVTLKTVYDRSELIQRAIAT
                     LRHTLLEESLIVAAVCVVFLLHVRSALVAILMLPVGVLIAFVALHLLGMNSNIMSLGG
                     IAIAIGAMIDAAIVMIENAHKHLERLPDDATPAARAAALLAACREVGPALFFSLLIVT
                     VSFLPVFTLEGQEGRLFAPLAWTKTFAMAGAALLSVTLVPVLMGLFIRGRVLPEARNP
                     VNRLLIRVYRPVIAAVLAWPRLTVVLAGAVLALTAIPATRLGAEFMPPLDEGTLLYMP
                     TSLPGLSVTKAAELLQIQDRIIKSFPEVDSVFGKAGRAGTATDPAPVEMFETVITLKP
                     HEQWRPGLTTDGLVAEMDKALQFPGVANAWTMPIKARTDMLATGIRTPIGIKVFGRDL
                     DEMERLAKDIETVVRQVPGTTSAFAERLTGGLYLDIEPDRARLARYGLSVGELQEVIA
                     GALGGEAVTTTVEGRERYAVAVRYPRELRSDPERIAREVLVPVDGGAMVPLGQVATVS
                     IARGAPGIRTENAQLAAYLYVDLRDRDIGGYVADAQRAVAEQVHFPPGAYVTWSGQFE
                     YMERARERLQVVVPITLLSIFMLLYLNFRRLTETFIVMLSVPFALVGGVWLLAALGYN
                     LSVAVGVGFIALAGVAAETGVVMLIYLDHAWADALARCRAEGREASRADLLAAVTSGA
                     VERVRPKMMTVVAIMAGLLPILWSRGTGSEVMSRIAAPMVGGMLSSTVLTLAVIPALY
                     LLVKGRALAGRP"
     gene            14941..16104
                     /locus_tag="PLH85_07465"
     CDS             14941..16104
                     /locus_tag="PLH85_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64437.1"
                     /translation="MTSARSALRLCHAGMEGRTLDAFQIFLSRVDAFSCRITDENHAD
                     AAFIDADGDLGQYLLEGHRLLYPGRPMIVTSSRPTPNHDPFTIQVTKPVGLAAFSAAL
                     QRVHELLPRSRDDGRRAPQPPFYGDLGAPPDDLPAFTDAVEAETIFATRYVGSAPDVD
                     LDDPRQRERIYYLPENFLQGTLARALAHARDVERPVKLRGPLGTILYLDPTNESAYQP
                     LSPKALRAQAQVPTRGTIKQTTLDAGDVGPVARLDPRPLQVVEWEVALWASRGRLPRG
                     TDLDTPVRLVRWPNFTRLPVPEGALRIAGLWSRTPTTLRDTVHLLGLPQRHVFAFFSA
                     CAALDLLQLGDEGMPDRRGTGDPARTPERRGLLQRTLGRLLGARPDERPDPRP"
     gene            16101..16577
                     /locus_tag="PLH85_07470"
     CDS             16101..16577
                     /locus_tag="PLH85_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64438.1"
                     /translation="MSGRIIFSGPSGAGKTSAIDVASDIPAIWKGNADYGAMTLDDGG
                     VIHLCSSACETATGVSGDDSLGLILLIDNRRTAPDDDLLRHLRSFDAFIRRTAVAVGI
                     THMDQQPRPRIDLFHTVMSYAGHGGMPLFEVDARDRRDICLLVEAMVSVLDPVACA"
     gene            16700..17938
                     /locus_tag="PLH85_07475"
     CDS             16700..17938
                     /locus_tag="PLH85_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB64439.1"
                     /translation="MPRLFTPAFAAALMVPALCLAQASVSPLTLADADRLLLDANPAI
                     LQARHQLTGAHAGIDIAGARPNPQLTLGVSTIDTGGSRWRRPTDNSVRIDQTFERGDK
                     RDLRLKAAERGVQAADADLTNTIRLARIDLANAWIDLRTAQDVQRASDESAAFATRTA
                     QAAELRGKAGDLAGVDVARYAADAARVANDAVQAGLQLAQARIALSQILGNRIAPNQL
                     RAADTWPDEATNSVAANAERADIAAARRRLEQADALRGLARAQRSRDLSVGVQYEHTP
                     ASLNNAVSENTFGLSVSVPLFLGYDYRGDIARSEADYLMAEQELARIRAAVDGERLRL
                     KAEVAAADARYRRLQDIALPAAERAARGAEVAIQKGGMSLTDYLDTQRGLRAARIETI
                     QARADLARARQALALAGDTP"
     gene            17935..19095
                     /locus_tag="PLH85_07480"
     CDS             17935..19095
                     /locus_tag="PLH85_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB64440.1"
                     /translation="MNRKQIAIAAVVAIAVAAAVYLRITRVGEDTPDTPVVQKPSAEP
                     GLLRFPTAAPQLAQLKVDAAAVSPVPMDEPLAAKVAYDENATARLFAPVAGRVIEIHG
                     QIGDTVKAGAVLAVLDAPELGAAEADVSKADAEASRTKAAFERARSLFDAEVIARRDL
                     ESAESDWRQARAEADRARLRLANLTPGGSSVSGQRYTVRAPIGGVIAARHINPAMEVR
                     PDAQDPLFVVTDLSRLSVLVDVPERDLPLVTVGKAAVVEASAYPNRNFDGQVVHVAPT
                     LDPATRRVQARVSVDNREKLLKPEMYARVSLLADAREELPRIPTAALLVEGVKNFVFV
                     ERAPGEFEKREVTLAVQTRQFAYVWHGLKGGEKVVSAGALLLNAELASSSAR"
     gene            19101..22193
                     /locus_tag="PLH85_07485"
     CDS             19101..22193
                     /locus_tag="PLH85_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB64441.1"
                     /translation="MFDALITAALRQRAFFLIAALALLGYGIYAFRQISIEAFPDVQD
                     VQVQVVTQVPGQAPEEVERTVTLPIEREMSGVPGMTQLRSVSITGLSVVTLTFSENTG
                     DYFARQQVLERLQTVNLPPAIQPTLAPLTTAVGEIYRYVLESPPDTPLEEIRAVQDWS
                     IRPALRIVPGIADVVSFGGAVREIQVRIDPDLLRKYNVSLNDVSQALATASANGGGGL
                     LRRGDEALVVRALGLFNTLDDVRDVVIVARDGKPVRISDVAEVASGARPRAGIVAFND
                     RDDVIEGIVQMTKGGNAAKIVEALRAKVDEVNARLPEGYRIRPIYQRTELIGHTVATV
                     GENLLVGAFLVTGILLVFLRNLRGAVIVAAVIPLSLMFAFILMHAMGVSANLISLGAV
                     DFGVIIDSAVVLVEALMVRLAVDAKAEHHISSPVGRRLYALKHTCVELGQPILFSKAI
                     IIVAFLPIFTFQRVEGRIFTPVALTLSFALLGGLILTLTLVPTLLSFALNKDMAEKES
                     HWLHRLQENYRSLLLGLQARRRSMVVGSIALLITSLGLAPMLGSEFLPKLDEGNIWLT
                     ISLPQSSALGNTKDVERKVRAIIASYPEVKAVITHVGRPDDGTDPKGPNNMEILVDLK
                     PRGEWRFPYKDDLVDSMAARLSTIPGLPTNFSQVIQDNVEESLSGAKGEIAVKVFGPN
                     LEVLEAKGRQIADILNRIQGSADVAALPVGGQSEVDIRLHREALARYDINVTDVNALI
                     QTALGGSAVNAYYEGDRRYDVTLRFGAAFRDQVDDIATLPVPLADGVGSIPLGDLADV
                     ELRQGASRIQREAGSRNVIVKANLRGRDQGSFVAEAQAKVAAEVSLPKGYTITWGGQF
                     ENQQRAMGRLKVIVPITLGLIFSLLFWAFRDVRPAFLVLSMVPFTLIGGFAGLGLAGL
                     HLSVSAAVGFIAVAGISVQNGLIMVEQVIEISRRGASRAEAILEGAVLRLRPIIMTAL
                     MAGLGLLPAALSHGIGSETQRPFAVVIVGGIVSATLFTLLLLPVLLSFGGAQSLRDKE
                     "
     gene            22234..22926
                     /locus_tag="PLH85_07490"
     CDS             22234..22926
                     /locus_tag="PLH85_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB64442.1"
                     /translation="MNARVLIVEDDPLLADGVAQVLRDAGFVTDYVGTAEQAEASLAA
                     EAFDLLVLDIGLPGMDGLELLRRLRARSSAIHVLMLTARDALNERVRGLNAGADDYLT
                     KPFAAVELLARVNALARRSRGQRGARREFGPLILDEEAYRAWLDGEPLTLPGREWAVL
                     LFLLDNQGRVMSKERIAAAVCRWDQDMSENAVEVYISRLRAKLEPAGIRIRTVRGLGY
                     MLENLRDEPKPA"
     gene            22907..24271
                     /locus_tag="PLH85_07495"
     CDS             22907..24271
                     /locus_tag="PLH85_07495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB64443.1"
                     /translation="MSPSLRKSLLLWLLLPAGLAVLTFLPLAYHLVHQPAEEALDRAL
                     ADASLALVPHLEVRNGEPHFVFPPAAEQVLRADRVDQIYYLVLGPGERFVAGDAGLPH
                     QPEGEEIHGERVSFNSHFRGQPIRVTAVHHSVAGEPFIFVTAETLRKRNQLAVDLAFA
                     LAMPLFFFAAATGFTIWFGIRHALLPVEDIRRSLHGMEHHALQPLDALSAPAEIRPLV
                     VEFNAVLDRLERASDAQQRFVANAAHQLRTPLAGIRTQLELLLRDADPEQRRNRTTQC
                     IAALERLGHLIGQMLVLISAEPGGRDAAAGSLLDVPEIIRERSPDWIRLAEAKGIDLG
                     FELGPVRIRGDGLLLGELIANLVINAITYTPAPGEVTVRCRQSSDHAEVDVEDSGPGI
                     PAEARELVFERFYRLPTSTAPGSGLGLAIVREIAGRFGGRALIAAPPGGKGTLVRVSL
                     PASA"
     gene            24285..25250
                     /locus_tag="PLH85_07500"
     CDS             24285..25250
                     /locus_tag="PLH85_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HNB64444.1"
                     /translation="MKLVHRLLRRSALVLARTVGGRPFATRIRHLAAQHYPPSAAGRA
                     GEATRFHLRSLIFAGPTRSWFELLESDARLQHLVRLNPHLAEKIHRPYLRCHYSPSRR
                     LAALSTHYRFCLDHPQGGLLLHAAAGRTVPVGEILGKQDSVFRAVLTSEEQFMKEGEF
                     VIQLHDEHGRLYSIAAAIVGSRGGPVLSVGCMQGPGQGENGSARIKAATKAMWGLRPK
                     AAILLIAQNLAENFGLTGLVAVGNDHHIYRSWRKRRTIHTDYDALWADLGGTPRPDKD
                     FDFPVVPPVRPISDFPSGKRADAQRRQTLREALAVSIRSHLAAAT"
     gene            25441..26241
                     /locus_tag="PLH85_07505"
     CDS             25441..26241
                     /locus_tag="PLH85_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNB64445.1"
                     /translation="MPAAPTATEHDDAAPYPSITVPEPLLCRPSKGREAPIPLVDGEV
                     HLWQVQLPIRDTALCRAVESATTANERARAGRLPFEDDRLRQLYTRGMLRILLGHYLS
                     KSPNALNFPLNPWDKPLLEEDPGLHFNVSHAHSLALIGVTRSAPVGVDLEAVRPLDDR
                     EEQFRRTLCSDELSWIDRFSEPLRRNHEFMRLRTGKEAALKALGMGLARHLDSFGVVP
                     PPSPVGPGEVNGLRGRWTLHFPPLPQGFAGAVAIATDSAGPLRVFRVG"
     gene            complement(26256..26681)
                     /locus_tag="PLH85_07510"
     CDS             complement(26256..26681)
                     /locus_tag="PLH85_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNB64446.1"
                     /translation="MKTFSFCAALALALMPVASHAEADLSTYRAFGEKPGLVSLMDAF
                     MVNLLADGRTRPFFEHADQTRIKAQLVDQICAVLDGPCTYQGAPMGPLHAGLGIQQGH
                     FNALVEDLQQAMDAQGVPFRAQNKLLARLAPLHRDIVTR"
     gene            complement(26716..27579)
                     /locus_tag="PLH85_07515"
     CDS             complement(26716..27579)
                     /locus_tag="PLH85_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HNB64447.1"
                     /translation="MRFRVRIAPIVLAWGAAMPAFAASDKLLLTGGVSQLEGAAGGGL
                     TPWAVIAGYGTRDQIGASAFYTQVRSQDYGLGVSGIAVGIRDRLELSYAHQSFDTRDV
                     GGALGLGNGFSFNQEIFGAKLRLLGDAVLEQDSWLPQIAIGLQHKRNDQGAVLHAIGA
                     KKDHGTDFYVAATKLFLDQSLLANLTLRETKANQFGILGFGGDLKDRYTTQFEGSLAY
                     LLRRDLAVGVEYRTKPDNLGIAREDKAYDAFLAWQPVKNVAVTLAYANLGNIVIKDNQ
                     RATYLSVQLGF"
     gene            27711..28499
                     /locus_tag="PLH85_07520"
     CDS             27711..28499
                     /locus_tag="PLH85_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64448.1"
                     /translation="MTARRLPACLALLALLGLTALPAAWAALPDEIQVYADDLNAPGE
                     FGLEVHVNTSPRGQRDATYAGEVKNDHSLFVTPELSWGLNDTMDAGLYLPTARDAATG
                     NLYAAGAKGRFKWLPIRGDELQGGGFAGLNLEIARIARRFEEYRSAAELRAMLGWRTP
                     DWLLAFNPVLSWDLSDGADRRTGYGFAFKGTHRIGEGLAGGFEYYTDQGKVGERQPWN
                     AQSNKLFAVLDVERHGWGFNVGVGKGLSTDTEKLTFKGILEFAF"
     gene            28794..31031
                     /locus_tag="PLH85_07525"
     CDS             28794..31031
                     /locus_tag="PLH85_07525"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNB64449.1"
                     /translation="MAAGKSTIIYTLTDEAPLLATCAFLPVVRTFTAPAGVDIVKSDI
                     SVSARVIAEFSDMLPDELKMPNTLAELGRITLQPDANIIKLPNISASVAQLKACIKEL
                     QEKGYPIPDFPENPTTDTEKAIKARYGKCLGSSVNPVLREGNSDRRAPAAVKNYARKN
                     PHSMGEWKQWSQTHVSHMEHGDFYHGEKSLTLDKARRVRMELIAVGGKTTVLKPEVKL
                     LEGEIIDSMFMSKTALCNFYEQQLEDCRESGILFSLHVKATMMKISHPIVFGHCVKIY
                     YKDAFEKHAKTFEELGINVNNGMVDLYEKIKALPESKRDEIIRDLHACQEHRPRLAMV
                     DSAKGITNFHSPNDVIVDASMPAMIRSGGKMWGADGKQYDCKAVMPESTFARIYQEMI
                     NFCKWHGNFDPKTMGTVPNVGLMAQQAEEYGSHDKTFEIAEDGIANIVDIDSGEVLLT
                     QNVEAGDIWRMCQVKDAPIRDWVKLAVTRARNSNTPAVFWLDPYRPHEAELIKKVETY
                     LKEHDTTGLDIKILSQVRAMRFTLERVIRGLDTISVTGNILRDYLTDLFPIMELGTSA
                     KMLSIVPLMAGGGMYETGAGGSAPKHVQQLTQENHLRWDSLGEFLALAVSLEELGIKT
                     DNAKAKLLAKTLDAATGKLLDNNKSPSPRTGELDNRGSQFYLSMYWAQELAAQTEDAE
                     LAATFAPLAKALTDSEDQIVAELKMVQGRPVDIGGYYRADSEKCKAVMRPSPTFNAAL
                     KAART"
BASE COUNT         4842 a        12060 c         9847 g         4595 t
ORIGIN      
        1 tcgatttcct tggccacgga cacaccgtcc ttggtcacgg tgggggcgcc gaaggaacgc
       61 tccagcacca cgttgcggcc cttggggccg agggtcacct tgaccgcgtc ggccaggatg
      121 ttgacgcctt cgaccatgcg ggcgcgggcg gaatcaccga acttgacttc tttggctgcc
      181 attcttgatt actcctgaat tctgggggac ggggaagacg gaacgcggga ggctcaggcc
      241 tcgaccacac ccatgatgtc ttcttcgcgc atgaccagca gttcctggcc atcgaccttg
      301 acggtctggc cggcatactt gccgaacagg acgcggtcgc caaccttcac ttccagcttg
      361 cggaactcgc cgttgtcgag aaccttgccg ttgccgacgg ccagcacttc gccctgatcg
      421 ggcttttcac cggcggagtc ggggatgacg atgccgctgg aggtggtgcg ttcggcttcg
      481 atacgcttca cgatgacgcg atcatgcaag ggacggattt tcatgtagag gtctcctact
      541 cgattcaaac cgggcaaacc tgcccgacca caattcgtgg ccagcctcgg tgctggcgct
      601 accgggagcc ggctgttagc actcaccccc gtcgagtgct aataataggg gcggcatggc
      661 gccatttcaa gtggggcatg cggaaataaa gatgccgacc catcgaacgc gccataaaca
      721 cagaacagag cgccgtctcg cccctccgcc gaggccgggc ctgcgcccca cgcagggtcg
      781 cgcggggccg cacgagggga cgcctgggga cgcctgggga cgcctgggga cgcgcccagc
      841 gtgtcgcgaa accgccattc tgcgggcggt ccgctggcgt cgcggggcga tggcccgcag
      901 gtcggatgaa cccgtcgtca gcggcgtggt gcgtcgcccg cttgcaaata gatagatagc
      961 tatctattat ttaaacgctt actgccatgg gtccctgctc cggtcctgcc cttccctctg
     1021 ccagtcccgc ccgacgatga cgactccacc ttccgccctg cgccgccagc tcacgaccct
     1081 cctgatccgc gccgcgcgcc tgtaccgcaa ggaggtcaat cgggcgctca cgacctatgg
     1141 catctcggat gctcaggcca tcccggtcct gcatatcgcc cgccacggcg gcggcatgcg
     1201 tcagcacgaa ctggcggagg agatcgggat cgaggggcct tcgctggtcc ggctgctcga
     1261 tcaactctgc gcgcacggcc tggtggagcg acgcagcgac ccccaggacc gccgcgcccg
     1321 cacccttcac ctcactgatg ccggtgaagc actcgccgcc cgcgtcgaga ctgcgctggt
     1381 cgggatccgc ggccgcctcc ttgccgccgt gagcgaggcg gacctgagca cctgtctccg
     1441 ggtgctggaa agcctgctcc atcagttgga tgtcgggcca tcccaccacg atgatccggc
     1501 ctgagcccct gttgcgcctc gactggcggg acgtccagtt ctcggtggtc agtctggccg
     1561 ccgccctggc cgccctgtac tggagcatga gcctggatct ccagcagccc tactggtcga
     1621 tgctgtccgc ctacgtggtg agccagcccc tggcagccgc cgtacgctcc aaggctgtct
     1681 accgtctggc cggcaccctt ctgggcgctg cgatgacggt gctgctgctg cccgccctcg
     1741 ccaatacgcc cgaactgctg tgcgcagcga tggccctgtg ggtcggcgcc tgcctgtcca
     1801 tttcactgct ggatcgctca ccgcgcagct atgtcctcat gctggccggc tacacggccg
     1861 ccatcgtcgg cttcagcagc gtcaatcaac ccgggcagat cttcgacctt gccgtggcac
     1921 gtgccgagga gatctccatc ggcatcctct gcgccacgct ggcccatagc ctgtggtttc
     1981 cccgctcggt gggcgaggtg ctcgagcagc gcatccggga ctggctcggt gaggccgacc
     2041 gctgggcgct cgaccttttc gccgccgatg acccgagcgc cggcgttccg catcgggccc
     2101 atctggctgc ggcggccacg gatatccacc tgttggcggc gcacctgccc ttcgacacct
     2161 cccatctgcg cgaaaccacc gccatcgtcc gggcgctcca cgaccacatc ctgctgctga
     2221 tcccggtcct gtccagcctc tcggatcgcc tcgccgccct ccacgggatg cgtgcccccg
     2281 tcgatccgcc cacgcaggac cgcctgaccc gcgtcggtgc ctggattcgc gcggactgcc
     2341 ccgcggcgga gggccgcgcg ctcagcgcca tgctggccgc agacctggac acaccggcag
     2401 cggcgcctga cgccgcctcc tggtacgcac tcaaccggaa cggtctgacc acacgcctgc
     2461 tcgatctggt gcagatccag accgaatgcc gtcagctgct cgcccacctg cacgaaccgg
     2521 acgcccccct cgcgcccgcc ctgcgggacg ccctcggcca ggccggcgca cgtccgctgc
     2581 acagtgactg gcggctggcg ctcacgtcgg gcgcggtcgc caccaccgcc atcctcatct
     2641 gctgcgccgc gtggatcgga ctcggctggt ccgaaggcgg tgccagcgca atgctcgccg
     2701 cgatcctgtg ctgtctgttt gccagcctcg acgaccccgc accggccatc cgcaagttcg
     2761 gcgtcgccat ctgtatcgca gtgagccttg ccgcgctcta ccagtttgcg gtgtttccgg
     2821 ccatcaacac cttcccgctc ctcgcccttg tcctggcacc gccgctgctc gccatcggcg
     2881 ccgcgacact caacccgcgc ctggcctcgt cggccctctt catcctcctc aacttctgca
     2941 atttcatggc catccaggag cgcttcacgc cggatctagc ggtcttcctc aacaacaacc
     3001 tgtcccagtt cttcggcgtc ttcgttgcca tttacgtcac ccgcgcggcc cgctccctga
     3061 gtaccgcgtc cagcgcccgc cgcctggccc gggccacctg gcaggacctg gcgcgcttcg
     3121 cccacggaga cgtccgcgag cctcaccaga acctcacggc ccggctggtg gaccggatcg
     3181 gcctgctggc accccgcctg gcggccctca gggatcaaga cgccgagctc gccgacggcg
     3241 atgtgctgcg ggaactgcgc atcggcatgg acctgagcac cctgcaggac gcccatccgc
     3301 ccctgcccgg gatcgcacag acgcaggtga ccgaattgct ccacgccgtc ggccgccact
     3361 accagcagcg tcccgacgga accgcccgcc cgccgcagga cctctgcgcg ctgctcgacg
     3421 gctgcatccg gcacctcgca ggcccccccg gtcagggccg cgcccttgcc gccctcgtgg
     3481 gattgcggcg caacctcttc ccggacacgc cctatgccgc cggaggactg aacccatgaa
     3541 cggcgagatc gaactgttcg gcgtctatct cagcgccgaa ctgagcacca gcatcgccgc
     3601 cctgctgctc accttcgggc tgcagcgggg cctcgtgtgg ctcggactgc tccgccgcgt
     3661 atggcatcga ccgctcttcg agaccgcgct gttcatcatc gtctggcgac tggtggtggc
     3721 cgcctgggcg ctgcactcac cctgagaccc tccgcggaga tcgcccccgt gaacacgacc
     3781 ttcctctcca aagccgctcc cgtgctgctc acactggcca tcgtggcggt cgccctgatc
     3841 gccggccggc agctctggat ccactaccgc agcgagccgt ggacccccga cggccgtgtc
     3901 cgcgccgaca tcgtacagat tgcgcccgac gtctcgggca ccgtcacgga cctcgccgtc
     3961 ggtcatgacc agcccgtcaa ggccggccag ttcctgttcc agctcgaccc ggaacgttat
     4021 gccctggcct tgcacgacgc agaatccgca gagcgcgtcc aggaagcccg cctggcccag
     4081 ctgcagcggg aggtgcgccg taaccagacg ctcggcgaac tggtcagccg cgaagcggcc
     4141 gaacagaccc aggcccacct ggatgaggcc cgcgccgcgc tcgcccaggc ccgcgcgcag
     4201 cgggaactgg ccgcgctcaa cctcaagcgt acccggatcg tcgcgccggc agacggcacc
     4261 ctgtccgacc tcaccctgcg cgtcggcaac tatgtggcgg ccggcaagcc ggtgatggcc
     4321 ctgatcgact cccgctccct gcgcgtcgaa ggctatttcg aagagaccaa gctgccccgc
     4381 atcaacgtcg ggcagacagc ccgcgttcac ctgatgggcg acgaccggat cctgacgggg
     4441 cacgtacaga gcatcgcggc gggcatcgag gaccgggacc ggacgtccgg cagcagcctc
     4501 ctgcccaatg tcaacccgac cttcagctgg gtccggctgg cccagcggat tcccgtacgc
     4561 atcgccctcg acgcccccca cgaccctgcg ctgatcgccg gccgcacggc cacggtgacg
     4621 ctcctcccgc cgaccgccga acgcgacggc ggccccgcgc gatgacgccc cgcgccgccc
     4681 tcgtctcggg cgccatcgcg ctgctgctca gtgcctgcag cgccgtcggg cccgactatc
     4741 gcctacccga cgctgcacag ctgcgcagcc ccgacgcccg ccaggccttc caagaagccg
     4801 gccagccgcc cttcggtcgc gacgatctgc ccgacgcctg gtggcgcctg tacgaggacc
     4861 ccgccctgaa cgacctgatc gcgcgcgcgc tggtcgccaa tgccgggctc agggcggcgg
     4921 cggccaacct cgcccgcagc gaagccgtgg tccgggaagt cgacggcctg cgcgatccgt
     4981 ccctgggcgc cggcgtcgcg atcgagcgcg ctcaggtttc ggctgaatcc tatctgctcg
     5041 aacacaaggt ccccgtcttc aacctgggca acgcgggtct gcgggcccac taccaggtgg
     5101 acctggccgg acaactgcgg cgggccctgg aagtcgccga ggcggaggcc ggcgccgtgc
     5161 aagccgccgg cgatctggcc cggatcagtg tggtggccga caccgcgctg gcctacatgg
     5221 acgcctgcgc ggccggcgag gcgctcgatc tcgcccgccg cgccctggca ctgcaggtcc
     5281 gcgaacgcga ggccatcgcc cgcctggtgg ccggtggacg gcgcatcccg ctggatctgc
     5341 cgcgggccgc agaacaggaa gaccagctgc gcgccgccct gcccgccttc gccgcgcgtc
     5401 gccgcagcgc cgcctaccgc cttgccgcgc tcacgggcca gtcccccgcc cgcgcgcccg
     5461 ctgccgctga ggcctgccgg cacctgcccg atctcgccca gccgattccc gtgggtgacg
     5521 gcgccgccct gctgcgccgc cggcccgatg tgcgccaggc agaacgccgg cttgccgcgg
     5581 catcagcccg catcggggtg gccacggcgg ccctctaccc cagcctgtcc ttcggattga
     5641 gcgccggcgg cacgggcatc ctcagccacc tgggcgaagc ccccacccag cgctggagcc
     5701 tgggccccgt gctgagctgg agcctccccg gagaagaagc ccgcgcccgc gtggcccagg
     5761 ccgatgcggg cgcggccggc gcactggcga ccctcgatca ggtcgtcctg aacgccctcc
     5821 aggaaaccga gagcgccctc gccaccctgg cccgcgaccg ggaccgccac gcagcactgc
     5881 agcaggcccg cgcccacgct gaaactgcgc gccgccaggt gcacaccttg tatcaggcgg
     5941 gccgtacggg tgtgctggac gacctggacg ccgagcgccg cctgctcgcc gcggacgcgc
     6001 gcctcgccgc gcatcgtggc cgcctcgcaa ccgatgaaat ccgcctgttc cttgcactgg
     6061 gcggcggctg gcagccgggc gaggtgcaca cgaccccgca ataagtggat cgcaaggtgc
     6121 tcgcgcgaac ccggccgccc ggcgtctcgc cgccctacgc ccgtctgggc cgccatcagc
     6181 tgccgccggc cggcggcagg cgcatctgga agaccgtgcc atggacgccg ctgcgcacga
     6241 ccacctcgcc cccatgggcg cgcacgatgg agcgggtgat ggcgagcccc aggccggcgc
     6301 cgtctccgtc gctgccatga cggcgcgccg ggtctgcccg gtagaagcgg tcgaagaggc
     6361 gcggcaggtg ttccgccggg atcggttcgc cgtcattggc gaccgcgacg accacccggt
     6421 cctccgcgtt cccgagggtc acccgcacgg caccacccgc cggcgtgtgg cgcagcgcgt
     6481 tggagagcag gttgcccagc gcacggcgca gcatcaggcg gtcgccggcc acgcgcccgg
     6541 cgcccgcctg ggacaggctc acaccgacct cctcggccag cgccgcatag aactcgaaga
     6601 gggcacgcac ctcgtccgcc agctccacgg gttcccgcgc cggcacgatg aggccgttgt
     6661 cggccttggc caggaagagc atgtcggcaa tcgtgcgggt caggcgctcc agctcctccg
     6721 cgcccgattc cagcacctcc cggtattcgg cggcgctgcg cgggcgcgac agggcaacct
     6781 gggtctgggt catcaggttg ctcaccggcg tgcgcagctc gtgggccagg tccgacgaga
     6841 aatccgacag gcgccggaag gactcctcca gccgcgccag catggcgttg agggattccg
     6901 ccagggccgc cagctcgacc ggcaccgaat ccaccgccag gcgcgggtcg aggcgctggg
     6961 cgctgacctg ggcggcggtg cggcgcatcg cctcgagggg cgccaggccg ctgcgggccg
     7021 cggcccagcc gagcagcccg gtggccagcg cgctggccac caccacgccc cacagggtgc
     7081 gccggaagcc gtccatgaag gcctgatgat gggagatgtc cgtcgccacc cgggcccgca
     7141 gcgggccggc gcccgccggc gcagcgggca actcggcggc caggccgcgg aaccagtgcc
     7201 cggaggcgtc ctgccacacc tgaggcccgg gcgcgccgat caccacatcg gacggaaaga
     7261 cggcgccggg cgacgcggcg agcacccgcc cgtccggacc gcgcagctcc atcatcggcg
     7321 gatgcaggtg ggcgccttcc accaggccgc cctcgccgcc cgcttccccg tgaaggtggc
     7381 cgagggccag cccgcggcgc agatctgcca gctgaccgta cagggtggcc cggtcgagct
     7441 cctcgaaatg acgctccacc gaggcgccga cgatgaagcc gaggatcagc agcaccgcgc
     7501 tcgacgccag ggcgaagagc agggtcaggc ggaccgtcag ggacaggcgc cggttcagtc
     7561 ccacggcgtt tcctccagca cgtagcccat gccccgcacg gtgcggatca gacgcggcgt
     7621 gaagccctcg tccaccttgg cgcgcaggcg gcgcaccgcc acctcgatca cgttggtgtc
     7681 gctgtcgaag ttcatgtccc acacctggga ggcgatcagg gagcgcggca gcacctcgcc
     7741 gcgccggcgc aggagcagct ccagcagggc gaactccttg gcggtgaggt cgatgcgccg
     7801 gccggcgcgg gtcacccggc ggcgcggccg gtccagctcc aggtcggcca ccttcagcac
     7861 gtccggctcg cgcgcccgcg cgccgcgccg cagcagggtg cgcacccggg cgagcagctc
     7921 cgagaaggcg aaaggcttga ccaggtagtc gtcggcgccc agctccagcc ccttgacccg
     7981 gtcgtccacc tgatcgcggg cggtcaggaa cagcaccggc agatccttgc cggcgcggcg
     8041 caggccctgc agcacctgcc agccgtccgc gccgggcagc atcacgtcca ggatcaccag
     8101 gtcgtaggcc tccgtcaggc cgcggtgcag cccgtccagg ccgtcctgca cccagtccgc
     8161 cacgaagccc gcttccacca gtccctggcg caggtagtcg ccggtcttgc tctcgtcctc
     8221 gacaatcagg atcttcatcg tgcgtctccc gcgggcgccc cgccgcccct tgcctcccag
     8281 tgtcccgcag gatcgtgcgg gcggacgaag cttacagatt tgtaatccgg cagacagctt
     8341 cccgtcggcg cccatggccc acaatgacga cattcccgtc atccatcgtc cggatactgc
     8401 catgcgcctc cgcctgctgc ccgccgcccc cctcgccgcc gtcctcctcg ccgccctgcc
     8461 ggcgattgcc gccgagccca ccgcccacgc ggcccacgcg actgccgccc aggccctcac
     8521 cgacggcgtg gtgaagaaga tcgacaaggc cgccggccgt gtcaccctga cccacggccc
     8581 cctcaacggc atgcccggca tgaccatggc cttcgccgtg aaggacccgg cctggctgga
     8641 cacgctcaag cccggtgagc gcatccgctt tgccgccgac accgtcaacg gtgcgctcac
     8701 cgtggtgaag atcgaacgcc cctgaatcat ccgccgccat gaagcctccg cgcctcgtcc
     8761 atctcctgct tatcggatgc gccgtcgccc tgccggcggc agcctgcgcc caggaggctc
     8821 ccctcggccg cgacgtggag gggctgctcg cctgggcccg cgcccacagc ccggaactcg
     8881 ccgcgatgcg ccacgaggcc gacgccgccg ccgagcgcgt cgtccccgcc ggcgccctgc
     8941 ccgatccgcg cttccgcacc gaactgcgcg acatcacccg catgggcgag cagagccccg
     9001 ccctgctccc cgcccgcacc ggcagcaccc gttacctgct gatgcaggat ctgccctggg
     9061 ccggcaagcg cgacctgaag caggccgccg ccgaacagga cgcccgcagc gccagcggcc
     9121 gcgccgacgc cacctgggcc gacctgagcg cgcgcatcaa gatcacctgg acccagctcc
     9181 actacgttca ccagagcacc cgcctcaccc gcgaactgct ggacctcggc ggacgcatcg
     9241 ggcagatcgc ccaggtgcgc tacgccggcg gcctcgcccc gcagcaggac gccatccgcg
     9301 cccaggtgga actgaccggc ctgcaggccg acctggtcgc cctcgacaac gaaggccgcc
     9361 agctccaggt gcgcctcaac gccctgctcg cccgcccgtc cgccgcgccc ctcgccgcag
     9421 cggagcgcct gcgcccgctg ccggtcccgg cgcgtctcga tccggccgcc ctggaagacc
     9481 gcctgcgcgc ccgcaatccc cagctcttcg ccgacgaggc gcgcatcgct gccgccgagc
     9541 gcaaccaggc cctcaccctg cgcaaccgct acccggactt caccgtcggc atctcgccga
     9601 tccagtacca gaacgccgtg cgcgaatggg agctgatggt ggaactcaac ctgccgctgc
     9661 agcaggcctc gcgccgcgcc caggagcggg aagccgacag cctgctcgcc gccgcccgcg
     9721 cccgccgcga ggccaccgcc cagcaggccc tcggcgagct gggcgaggcc ctgtcggccc
     9781 tggacgccgc gcgccgcacc gaagccctgg ccagccagag cctgctgccc caggcggaac
     9841 tcaccttccg cgccgccctg gccggctacg agaccggcaa ggtggacttc accaccctgc
     9901 tcgacgccca gcgccagatc cgcctggccc gccagaaccg cctcaaggcc cagaccgagg
     9961 cgcaactgcg ccttgccgac atcgaacgcc tcgtgggaga agacctgtga gcgccccccg
    10021 ccccgccctg cgcctcgccg ccgcccttct tgccctggcc gccgccgcgg gcgccggtta
    10081 cggactcggc cagcgccacg gcaccgaacc cccggccgcc tcgcccaccc cggccgcccc
    10141 ggccgcgcgc aagctgctct actaccgcaa ccccatgggc ctgcccgaca cctcgccggt
    10201 gcccaagaag gaccccatgg gcatggacta cctcccggtc tatgaaggcg aggccgacgc
    10261 ggaggccgcc cccggacagg tccgcctcag ccctgaaaag atccagaagc tcggcgtgcg
    10321 caccgaagcc gtcaccctgc gcgccctgga ccgcagcgtg gacgccagcg gccgggtgga
    10381 gccggacgaa cgccgcgtgg tcgccatcgc gcccaagttc gaaggcttcg tggagcgcct
    10441 gcacgtgagc gtcaccggcc agccggtggc ccgcggccag gccctgttcg aggtgtacag
    10501 cccggagctg gtgtccgccc agcgcgaata cgccctcgcc gcccagggcg ccgacagcct
    10561 caaggacgcc ggcccggagg cccagaaagg cctgcgccag ctcgccgacg cggcccttca
    10621 gcgactgcgc aactgggaca tcccggacgc ccagatccgc gccctcaccc acagcggtga
    10681 ggcccgccgg acactcacct tcacctcacc ggcggccggc atcgtcaccg agaagaaggc
    10741 cgtgcagggc atgcgcttca tgcccggcga gaccctgtac caggtcaccg acctgtccgc
    10801 cgtgtgggtg gtggcggacg tgaacgagca ggacatcggc gcagtccgcc ctggcagccc
    10861 ggtgaagctg cgggtggacg cctaccccgg tgagaccttc gacggccgcg tgagctacat
    10921 ctacccgacc ctcaacgccg acacccgcag cgtgccggtg cgcatcgaac tggccaatcc
    10981 gagcctgcgc ctcaagccgg ccatgttcgt ccacgtgagc ctgcccgccg gcagcgctgc
    11041 accggtcctc accgtgccga gcgcggcggt cctcgacagc ggcacccgcc gggtggtgct
    11101 ggtggaacgg ggtgaaggcc gcttcgaacc ccgcgaggtg cgcctgggcg cccgcagcgc
    11161 ccgctacgtg gaggtactcg acggcgtgaa ggcggacgag cgggtggtgg tggccgccaa
    11221 cttcctcatc gacgcggaga gcaacctgca ggccgcgctg ggcgccttca cggccaccgg
    11281 cggcacgccg ccggccgccc aggccgcgag cgtggtgcat cgtgccgaag ggcaggtgga
    11341 cggcgccgac gccgcggccg gcacggtgac cctcagccac ggcccggtgg ccagcctcaa
    11401 ctggccggcc atgaccatgg acttcgccgt cgccaacccg gccctgcagg cggcgctcaa
    11461 gcccggcgcg cgggtggcgg tggacttcgt cgagcgtaag cccggcgagt gggtcatcac
    11521 cgcggtgcgc ccggccgccg gccgctgagg gcgacaccat gctggcagcc ctcatcgact
    11581 ggtccggacg caaccgcttc ctggtgctgc tggcgaccct ctgcgtggtc ctcgccggcg
    11641 tctacgccgt gctgcgcacg cccatcgacg ccctgccgga cctctccgac gtgcaggtca
    11701 tcgtctacac cgagttcccc ggccaggcgc cccaggtggt ggaggaccag atcacctacc
    11761 ccctcaccac tgccatgctg tcggtgccaa aatcccgcgt ggtgcggggg ttttccttct
    11821 tcggcgtgtc cttcgtgtat gtcattttcg aggacggcac ggacctctac tgggcccgct
    11881 cgcgggtgct ggaatacctc agcgccgccg ccggacgcct gcccgcgggc atcatgcccc
    11941 agctcggccc ggacgcctcc ggcgtgggct gggtgtacca gtacgccgtg ctggcccggg
    12001 acaagaccct cgccgaactg cgcagcctgc aggactggac cctgcgctat cccctcgcca
    12061 aggcccgcgg cgtggccgag gtggcgtcgg tgggcggctt cgtgcagacc taccaggtca
    12121 ccgtcgatcc ggtgaagctg cgcgcctacg gcatacccct gtccagggtc gcccaggtga
    12181 tccgcgcctc caaccgggac gtgggcggcc gcgtggtgga gatggccgag accgaataca
    12241 tggtgcgcgg gcgcggctac ctgcgcggcg tggacgacat cgccagcctg gtggtcaagg
    12301 cggaacgggg cacgccggtg ctggtccgcg acatcggccg ggtggaactc gtgccggacg
    12361 agcgccgcgg cctggccgag ctggacggcg aaggtgaggc ggtgggcggc atcaccgtgg
    12421 cgcgcagcgg gcagaacgcc ctggacgtga tccgccacgt caaggccacc ctcgccgacc
    12481 tggcgcccgg cctgcccgat ggcgtcacgc tgaagacggt gtacgaccgc tccgagctga
    12541 tccagcgcgc catcgccacc ctccgccaca ccctgctgga agaatccctc atcgtcgcgg
    12601 cggtctgcgt ggtcttcctg ctccacgtgc gcagcgccct ggtggcgatc ctgatgttgc
    12661 cggtgggcgt gctgatcgcc ttcgtcgccc tccacctgct cggcatgaac tccaacatca
    12721 tgagcctggg cggcatcgcc atcgccatcg gcgccatgat cgacgccgcc atcgtgatga
    12781 tcgaaaacgc ccacaagcac ctggaacgcc tgccggacga cgcgaccccg gcggcgcggg
    12841 ccgccgccct gctggcggcc tgccgcgagg tgggaccggc cctgttcttc tccctgctga
    12901 tcgtcaccgt gtccttcctg ccggtgttca ccctggaagg ccaggaaggc cgtctgttcg
    12961 ccccgctggc atggaccaag accttcgcca tggccggcgc agcgctgctc tccgtcaccc
    13021 tggtgccggt gctgatgggc ctgttcatcc gcggccgggt gctgccggag gcccgcaacc
    13081 cggtcaatcg cctgctcatc cgcgtctatc ggccggtgat cgccgccgtg ctggcgtggc
    13141 cgcgcctcac cgtcgtcctc gccggggcgg tgctggccct caccgcgatc cccgccaccc
    13201 gtctgggcgc ggagttcatg ccgcccctgg acgaaggcac cctgctctac atgcccacct
    13261 ccctgcccgg cctgtcggtc accaaggccg ccgagctgct ccagatccag gaccgcatca
    13321 tcaagagctt cccggaggtg gacagcgtct tcggcaaggc cggccgcgcc ggcaccgcca
    13381 ccgatccggc acccgtcgag atgttcgaga cggtcatcac cctcaaaccc cacgagcagt
    13441 ggcggcccgg cctcaccacc gatggcctcg tcgccgagat ggacaaggcg cttcagtttc
    13501 ccggcgtcgc caacgcctgg acgatgccca tcaaggcgcg caccgacatg ctcgccaccg
    13561 gcatccgcac gcccatcggc atcaaggtct tcggccgcga cctggacgag atggagcgcc
    13621 tcgccaaaga catcgagacc gtcgtacgcc aggtaccggg caccaccagc gccttcgccg
    13681 aacgcctcac cggcgggctc tatctggaca tcgaaccgga ccgggcgcgc ctggcccgct
    13741 acggcctgtc cgtgggtgaa ctgcaggagg tgatcgccgg cgccctcggc ggcgaggccg
    13801 tcaccaccac cgtggaaggc cgcgagcgct acgccgtggc cgtgcgctac ccccgcgagc
    13861 tgcgcagcga cccggaacgc atcgcccgcg aggtgctggt gccggtggac ggcggcgcca
    13921 tggtgcccct cggccaggtg gcgacggtgt ccatcgcccg cggcgcgccg ggcatccgca
    13981 ccgagaacgc ccagctcgcc gcctatctct acgtggacct gcgcgaccgg gacatcggcg
    14041 gctacgtggc cgacgcccag cgcgccgtcg ccgagcaggt acactttccg cccggcgcct
    14101 acgtcacctg gagcggccag ttcgaataca tggagcgggc ccgcgagcgt ctgcaggtgg
    14161 tcgtgccgat caccctgctg agcatcttca tgctgctcta tctcaacttc cgccgcctca
    14221 ccgagacctt catcgtgatg ctgtcggtgc ccttcgccct ggtgggcggg gtgtggctgc
    14281 tggccgcgct cggctacaac ctgtcggtgg cggtgggcgt gggcttcatc gccctggccg
    14341 gcgtcgccgc cgagaccggc gtggtgatgc tgatctacct ggaccacgcc tgggccgacg
    14401 ccctcgcccg ctgccgcgcc gagggccgcg aggcgagccg cgccgacctc ctcgccgccg
    14461 tcacctccgg cgccgtggag cgggtgcgcc ccaagatgat gaccgtcgtc gccatcatgg
    14521 ccggcctgct gcccatcctg tggagccgtg gcaccggctc cgaggtgatg agccgcatcg
    14581 ctgcgcccat ggtgggcggc atgctctcct ccacggtgct caccctcgcc gtcatcccgg
    14641 cgctctatct gctggtcaag ggccgcgccc tcgccggcag gccgtgacaa cccgtcgctc
    14701 cgcggttcac gcccctctgc cgcccatccc taggcggcgg aatacccatg aattaaatat
    14761 ggttttgtaa aacactgtta acgtgacaga agttgccaat gcgaaggcat ccggccggcg
    14821 gtgatccacc tatcattccc gcaaagccgt cacgggcgct ccctccgccc cgcctcctcc
    14881 gacggaccgg tcacaccggc cctcgcggca gacggcaccc aaccgagaag gatgcgtgtc
    14941 atgacctctg cccgttccgc cctgcgcctg tgccatgccg gcatggaggg ccgcaccctg
    15001 gatgccttcc agatcttcct gtcccgcgtg gatgccttct cctgccgcat caccgacgag
    15061 aatcacgccg acgccgcctt catcgacgcg gacggcgacc tgggccagta tctgctggaa
    15121 ggtcaccgcc tgctctaccc gggccggccg atgatcgtca cctccagccg tcccacaccc
    15181 aaccacgacc ccttcaccat ccaggtgacc aagccggtgg ggctcgccgc attctcggcc
    15241 gccctgcagc gggtccacga actcctgccg cgcagccgcg acgacggtcg ccgcgccccc
    15301 cagccgccct tctacggcga tctgggcgcc ccgcccgacg acctgccggc cttcaccgac
    15361 gccgtcgaag ccgagacgat cttcgccacc cgctacgtgg gctcggcacc cgacgtggac
    15421 ctggacgacc cccgccagcg cgaacgcatc tactacctcc ccgaaaactt cctgcagggc
    15481 acgctggcac gggccctcgc ccacgcccgt gacgtggaac gccccgtcaa gctgcgcggc
    15541 cccctcggca ccatcctcta tctcgacccg accaacgagt cggcctacca gcccctcagc
    15601 cccaaggccc tgcgcgccca ggcccaggtg cccacccgcg gcaccatcaa gcagaccacc
    15661 ctcgacgccg gtgacgtggg ccccgtcgcc cgcctcgacc cgcgaccgct gcaggtcgtc
    15721 gagtgggaag tagccctgtg ggcctcccgc ggtcgcctgc cgcgcggcac cgacctggac
    15781 actccggtgc gactggtgcg ctggccgaac ttcacccgcc tgccggtgcc cgagggcgcc
    15841 ctgcgcatcg ccggcctgtg gtcgcgcacg ccgaccaccc tgcgcgatac cgtgcatctg
    15901 ctcggcctgc cccagcgtca cgtctttgcc ttcttcagcg cctgcgccgc cctggacctg
    15961 ctgcagctcg gcgatgaggg catgccggac cgccgtggca cgggcgatcc ggcccggacg
    16021 ccggaacggc gcgggctgct gcagcgcacc ctcggccgcc tcctgggcgc ccgccccgac
    16081 gagcggccgg acccgcgccc gtgagcggac ggatcatctt cagcggaccg tccggagcgg
    16141 gcaagacctc ggccatcgac gtcgcgagcg acatcccggc catctggaaa ggcaacgccg
    16201 actacggcgc catgaccctc gacgacggcg gcgtgatcca cctgtgcagc agcgcctgcg
    16261 agaccgccac cggcgtcagc ggcgacgaca gcctcggcct gatcctgctc atcgacaacc
    16321 gccgcaccgc gcccgacgac gacctgctgc gccacctgcg cagcttcgac gccttcatcc
    16381 ggcgcaccgc ggtcgccgtc ggcatcaccc acatggacca gcagccgcgc ccgcgcatcg
    16441 acctgttcca cacggtgatg agctacgccg gtcacggcgg catgcccctc ttcgaggtgg
    16501 acgcccgcga ccggcgggac atctgcctgc tggtggaagc catggtcagc gtcctggacc
    16561 cggtcgcctg cgcctgacac gccgcagact gcgtccgcca cggcgaacgc cccggacgca
    16621 agattcgcgc aaggatttcg tcagcacatc gacagagagg cgccctacac tgcgggcctg
    16681 atcctcgttg cgagttcgca tgccccgcct ctttaccccg gctttcgcgg cggccctgat
    16741 ggtgcccgcc ctgtgcctgg cccaggcgtc cgtgagcccc ctgacgctgg ccgacgccga
    16801 tcgcctgttg ctggacgcca atccggccat cctgcaggcc cgccaccaac tgaccggagc
    16861 ccacgccggt atcgacatcg ccggtgcacg ccccaatccg cagctcacgc tgggtgtcag
    16921 cacgatcgac acgggcggca gccgctggcg tcgaccgacg gacaactccg tccgcatcga
    16981 tcaaaccttc gagcgcggcg acaaacgcga tctgcgcctg aaagctgcgg aacggggtgt
    17041 gcaagctgcc gacgcggacc tcaccaacac catccgcctg gcccgcatcg acctggccaa
    17101 cgcctggatc gacctgcgca ccgcccagga cgttcagcgg gccagcgacg agagtgccgc
    17161 ctttgccacg cgtaccgccc aggccgccga actgcgcggc aaggcggggg atctggccgg
    17221 tgtggacgtg gcccgttacg ccgccgatgc cgcccgcgtc gccaatgacg ctgtgcaggc
    17281 cggtttgcag ttggcccagg cgcgtatcgc tctctcccag atcctgggca accggatcgc
    17341 ccccaaccaa ctgcgcgccg ccgacacctg gcccgacgag gcgacgaatt ccgtcgccgc
    17401 caacgccgag cgcgccgata tcgctgccgc ccgtcgccgc cttgaacagg cagacgcatt
    17461 gcgagggctg gcccgcgccc agcgcagtcg tgacctgtcc gttggcgttc aatacgaaca
    17521 tacgccggcc agcctcaaca acgcggtgtc cgaaaatacc ttcggcctca gcgtcagcgt
    17581 cccgctcttc ctcggctacg actaccgcgg cgacatcgcc cgcagcgaag ccgactacct
    17641 gatggccgag caggagctcg cccgcatccg cgccgctgtg gacggcgaac gcctgcgcct
    17701 caaggccgag gtcgccgccg ccgacgcccg ctaccggcgc ctccaggaca tcgccctgcc
    17761 cgccgccgag cgggccgccc gcggcgccga ggtcgccatc cagaagggtg gcatgagcct
    17821 caccgactac ctggataccc agcgcggcct gcgcgccgcc cgcatcgaaa ccatccaggc
    17881 ccgggccgat ctggcccgcg cccgccaggc cctggcgctg gccggagaca cgccatgaac
    17941 cgcaagcaga tcgccattgc cgccgtggtc gccatcgccg tggccgccgc cgtctacctc
    18001 cgcatcaccc gggtggggga agacacgccc gacaccccgg tcgtccagaa gccctcggcc
    18061 gaacccggcc tgctgcgctt ccccacggcc gcgccgcagc tcgcccagct caaggtggac
    18121 gccgccgccg tctcgccggt gcccatggac gagcccctgg ccgccaaggt ggcctatgac
    18181 gagaacgcca ccgcccgcct gttcgccccg gtggccggcc gcgtcatcga gatccacggg
    18241 cagatcggcg acaccgtgaa ggccggcgcc gtcctcgcgg tgctcgacgc ccccgagctg
    18301 ggcgccgccg aggccgacgt gagcaaggcc gatgccgagg cctcgcgcac caaggccgcc
    18361 ttcgagcgcg cccgcagcct cttcgacgcg gaagtgatcg ccagacgcga cctggaatcc
    18421 gccgagtccg actggcgcca ggcccgcgcc gaagccgaca gggcccgcct gcgtctggcc
    18481 aacctgaccc cgggcggctc cagcgtctcc ggccagcgct acaccgtgcg cgcaccgatc
    18541 ggcggcgtca ttgccgcgcg gcacatcaat cccgccatgg aagtccgccc cgacgcccag
    18601 gatcccctgt tcgtcgtcac cgacctgagc cgcctgtcgg tgctggtgga cgtgcccgag
    18661 cgcgatctgc ccctcgtcac cgtgggcaag gccgccgtag tggaagcctc cgcctacccc
    18721 aaccgcaatt ttgacggaca ggtggtgcac gtggccccca ccctcgaccc ggccacccgt
    18781 cgcgtccagg cgcgggtgag cgtggacaac cgggaaaagc tcctcaagcc cgagatgtac
    18841 gcccgcgtct ccctgctcgc cgacgcccgc gaggaactgc cgcgcatccc cacggccgcg
    18901 ctgctggtcg aaggcgtcaa gaacttcgtc ttcgtggaac gggccccggg cgaattcgag
    18961 aagcgcgagg tcaccctcgc ggtgcagacc cgccagttcg cctacgtctg gcacggcctc
    19021 aagggcggtg agaaggtggt cagcgccggt gcactgctcc tcaacgccga actggccagc
    19081 agctcggccc ggtaagcgcc atgttcgacg ccctcatcac cgccgccctg cgtcagcggg
    19141 ccttttttct gatcgccgcc ctggcgctgc tcggctatgg catctatgcc ttccgtcaga
    19201 tttccatcga ggccttcccg gacgtacaag acgtgcaggt ccaggtcgtc acccaggtgc
    19261 ccggccaggc cccggaagag gtggagcgca ccgtcaccct gcccatcgag cgggagatga
    19321 gcggcgtgcc gggcatgacc cagctgcgct ccgtgtccat caccggcctg tcggtggtga
    19381 ccctcacctt ctccgagaac accggcgact acttcgcccg ccagcaggtg ctggagcgtc
    19441 tgcagacggt caacctgccg cccgccatcc agcccaccct ggcgcccctc accaccgcgg
    19501 tgggcgagat ctaccgctac gtgctggagt cgccgcccga tacgccgctg gaggagatcc
    19561 gcgcggtgca ggactggagc atccgcccgg ccctgcgcat cgtgcccggc atcgccgacg
    19621 tggtcagctt cggcggcgcg gtgcgggaaa tccaggtacg catcgacccc gacctgctgc
    19681 gcaagtacaa cgtgagcctc aatgacgtca gccaggccct cgccaccgcc agcgccaacg
    19741 gcggcggcgg tctcctgcgg cgcggcgacg aggccctggt ggtgcgcgcc ctgggcctgt
    19801 tcaacaccct ggatgacgtg cgcgacgtcg tcatcgtggc ccgcgacggc aaaccggtgc
    19861 gcatcagcga cgtggccgaa gtggcctcgg gcgcccgccc gcgcgccggc atcgtggcct
    19921 tcaacgatcg tgacgacgtg atcgagggca tcgtgcagat gaccaagggc ggcaacgccg
    19981 ccaagatcgt ggaggccctg cgcgccaagg tggacgaggt gaacgcgcgc ctgcccgagg
    20041 gctaccggat ccgcccgatc taccagcgca ccgagctgat cggccacact gtcgccaccg
    20101 tgggcgagaa cctgctggtg ggcgccttcc tggtcaccgg catcctgctg gtcttcctgc
    20161 gcaacctgcg cggcgcggtg atcgtcgccg cggtgatccc cctgtccctg atgttcgcct
    20221 tcatcctgat gcacgcgatg ggggtgtcgg ccaacctgat ctccctgggc gcggtggact
    20281 tcggcgtcat catcgacagc gccgtggtgc tggtggaggc cctcatggtg cgcctcgcgg
    20341 tggatgccaa ggcggaacac cacatcagct cgccggtcgg acgacgcctg tatgccctca
    20401 agcacacctg tgtggagctg ggccagccga tcctgttctc caaggccatc atcatcgtcg
    20461 ccttcctgcc catcttcacc ttccagcggg tcgaggggcg gatcttcacg ccggtggccc
    20521 tgaccctgtc cttcgccctg ctgggcggcc tgatcctcac cctgaccctg gtgccgaccc
    20581 tgctgtcctt cgccctcaac aaggacatgg ccgaaaagga gagccactgg ctgcaccgcc
    20641 tgcaggagaa ctaccggtcc ctgctgctgg gactgcaggc ccgtcgccgg agcatggtcg
    20701 tcggctccat cgcgctgctc atcacgtccc tcgggttggc cccgatgctc ggctccgaat
    20761 ttctgcccaa actggacgaa ggcaacatct ggctgaccat cagcctgccc cagtcctcag
    20821 ccctggggaa caccaaggac gtggaacgca aggtgcgcgc catcatcgcc agctacccgg
    20881 aagtgaaagc ggtgatcacc cacgtggggc gcccggatga cgggaccgac cccaagggcc
    20941 ccaacaacat ggaaatcctg gtggacctga agccccgcgg cgaatggcgc ttcccctaca
    21001 aggacgacct ggtggacagc atggccgcca ggctgtccac catccccggc ctgcccacca
    21061 acttctccca ggtgatccag gacaacgtgg aagagtccct gtccggcgcc aagggcgaga
    21121 tcgcggtgaa ggtcttcggc cccaatctgg aggtgctcga agccaagggc cgccagattg
    21181 ccgacattct caaccgcatc cagggctcgg ccgacgtggc cgccctgccg gtgggcggcc
    21241 agagcgaggt ggacatccgc ctccaccgcg aagccctggc ccgctacgac atcaacgtca
    21301 ccgacgtgaa tgcgctgatc cagaccgccc tgggcggcag cgcggtcaat gcctactacg
    21361 agggcgaccg ccgctacgat gtgaccctgc gcttcggcgc agccttccgc gaccaggtgg
    21421 acgacatcgc gaccctgccg gtgcccctcg ccgacggcgt gggcagcatt cccctgggcg
    21481 atctggccga tgtggagctg cgccagggcg cctcccgcat ccagcgcgag gcgggcagcc
    21541 gcaacgtgat cgtcaaggcc aacctgcgcg gccgcgacca gggcagcttc gtcgccgaag
    21601 cccaggccaa ggtcgcagcc gaggtgagcc tgcccaaggg ctacacgatc acctggggcg
    21661 gccagttcga gaaccagcag cgcgccatgg gccgcctgaa ggtcatcgtg ccgatcaccc
    21721 tggggctgat cttctccctg ctcttctggg ccttccggga cgtacgcccg gccttcctgg
    21781 tgctctccat ggtgcccttc accctgatcg gcggtttcgc cgggctgggg ctggcggggc
    21841 tgcacctgtc cgtatcggcc gcggtgggct tcatcgcggt ggccggcatc tcggtgcaga
    21901 acggcctgat catggtggag caggtcatcg agatctcccg ccgcggcgcc agccgggccg
    21961 aggccatcct cgaaggggcc gtactgcgcc tgcgtcccat catcatgacc gccctgatgg
    22021 ccggcctcgg cctgctgccc gctgccctgt cccacggcat cggctcggaa acccagcgcc
    22081 cctttgcggt ggtgatcgtc ggcggcatcg tcagcgccac cctattcacc ctgttgctgc
    22141 tgccggtgct gctcagcttc ggcggcgccc agagcctgcg ggacaaggaa tgacgtgccg
    22201 ggcatccggc ccctggagtg aagcccccga accatgaacg cccgagtcct gatcgttgaa
    22261 gacgatcccc ttcttgccga cggtgtcgcc caggtcctgc gcgacgccgg attcgtcacc
    22321 gactacgtgg gcaccgccga acaggccgaa gcgtccctcg ctgcggaagc cttcgacctg
    22381 ctggtgctgg acatcggcct gcccggcatg gatggtctgg agctgctgcg ccggctgcgc
    22441 gcccgcagca gtgcgatcca tgtgctcatg ctcactgccc gcgacgccct caacgagcgc
    22501 gtgcgcggtc tcaacgccgg cgccgacgac tacctgacca aacccttcgc cgcggtggaa
    22561 ctcctcgccc gggtcaacgc cctggcgcga cgctcccgtg gccagcgggg tgcccgacgc
    22621 gagttcgggc cgctgatcct cgacgaggag gcctatcgcg cctggctcga cggcgagccc
    22681 ctgaccctgc ccggcaggga atgggccgtg ctgctcttcc tgctcgacaa ccaggggcgg
    22741 gtcatgagca aggaacgcat cgccgcggcc gtgtgccgct gggaccagga catgagcgag
    22801 aacgccgtcg aggtctacat ctcccgcctg cgggccaaac ttgaaccggc gggcatccgc
    22861 atccgcaccg tgcggggcct cggctacatg ctcgaaaacc tgcgcgatga gcccaagcct
    22921 gcgtaaatcc ctgctgctct ggctgctcct gcctgccggt ctcgccgtcc tcaccttcct
    22981 gcccctggcc tatcacctgg tgcaccagcc ggccgaggaa gccctcgacc gggccctggc
    23041 ggacgccagc ctggcgctgg tcccccacct ggaagtacgc aacggtgagc cgcacttcgt
    23101 ctttccgcct gcggccgagc aggtcctgcg ggcggatcgg gtggaccaga tctattacct
    23161 cgtgctgggc cccggcgagc gcttcgtggc cggcgacgcg ggcctgcccc accagcccga
    23221 aggcgaggag atccacggcg aacgggtcag cttcaactcc cacttccgcg gccagccgat
    23281 ccgcgtcacc gccgttcacc acagcgttgc gggcgagccc ttcatcttcg tcaccgccga
    23341 aaccctgcgc aagcgcaacc agctcgccgt ggacctggcc ttcgccctgg cgatgcccct
    23401 gttcttcttt gccgcggcca ccggcttcac gatctggttc ggcatccgcc acgcgctgct
    23461 gcccgtggag gacatccggc gcagcctgca cgggatggaa caccacgcgc tgcaacccct
    23521 cgatgccctc tccgcccccg ccgagatccg ccccctggtg gtggagttca acgccgtgct
    23581 ggaccgcctg gagcgggcct ccgatgccca gcagcgcttc gtggccaacg ccgcccacca
    23641 gctgcgcacg cccctggccg gcatccgtac ccagctcgag ctgctgctgc gcgacgccga
    23701 tccggagcag cgccgcaacc gcaccaccca gtgcattgcg gcccttgaac gccttggcca
    23761 cctcatcggc cagatgctgg tcctgatctc ggccgagccg ggcggccgcg acgccgctgc
    23821 cggcagcctc ctggacgtgc cggagatcat ccgcgagcgc tcccccgact ggatccgtct
    23881 cgcggaagcc aagggcatcg atctgggctt cgaactcggc cccgtgcgca tccgcggtga
    23941 cggattactg ctgggcgagc tgattgccaa tctggtgata aacgccatca cgtatactcc
    24001 cgccccgggt gaagtcactg tgcgctgccg ccagagcagc gatcatgcgg aggtggacgt
    24061 ggaagacagc ggaccgggca ttcctgcaga agcgcgcgaa ctggtgttcg aacggttcta
    24121 tcgcctgccc accagcaccg cgccgggcag cggtctgggg ctcgccatcg tgcgcgagat
    24181 cgccggccgt tttggcgggc gcgccctcat cgccgccccc cctggcggaa agggcaccct
    24241 cgtccgggtc agcctgccgg cctccgcctg agtccatcgt ctccatgaag ctggttcacc
    24301 gccttctccg gcgcagcgcc ctggtcctcg cccgcaccgt gggcgggcgg cccttcgcca
    24361 cccgcatccg ccatctcgcc gcccagcact acccgccctc cgcggcgggg cgcgccggcg
    24421 aagccacccg cttccacctg cgcagcctga tctttgccgg gccgacccgc agctggttcg
    24481 agctgctcga gagcgacgcc cgcctgcagc atctggtgcg cctcaacccc cacctcgccg
    24541 agaagatcca ccgcccctac ctgcgctgcc attactcgcc gtcacgccgt ctcgcggccc
    24601 tgagcaccca ctaccgcttc tgcctggatc acccgcaggg cgggctgctg ctccatgccg
    24661 ccgccgggcg gaccgtgcct gtcggcgaga tcctcggcaa gcaggacagc gtgttccggg
    24721 cggtcctcac gagcgaggag cagttcatga aggaagggga gttcgtcatc cagctccacg
    24781 acgaacacgg ccgcctgtac tcgattgccg ccgccatcgt tggcagtcgc ggcgggccgg
    24841 tgctctccgt cggctgcatg cagggcccgg gccagggtga aaacggcagc gcccgcatca
    24901 aggccgccac caaggccatg tggggcctgc gccccaaggc ggcgatcctg ctgatcgccc
    24961 agaacctggc cgagaacttc ggcctgacgg ggctggtggc ggtgggcaat gaccatcaca
    25021 tctaccgcag ctggcgcaaa cgccgcacca tccataccga ctatgacgcc ctgtgggccg
    25081 atctgggcgg cacaccgcgg ccggacaagg atttcgactt cccggtcgtt ccgccagtgc
    25141 gcccgatctc cgacttcccg tcaggcaagc gcgccgacgc ccagcgccgc cagaccctgc
    25201 gggaggcgct cgccgtgtcg atccgcagcc acctcgccgc ggcgacctga tcccggcgcc
    25261 tcaccgccac cgccaaagag caatacgccc cctgactgcg ccggtcatac gctgtaagca
    25321 taatcacctt acaagatttt aagatgtgaa tgggggcccc tctcgccgtg ggcggtattc
    25381 tgctgcccac acatcatctg gccagatcac ccggccggat ccatcggggg atcggggccc
    25441 atgcccgccg caccaactgc cacagaacac gacgacgccg cgccctaccc gtccatcacg
    25501 gtcccggagc cgctgctgtg ccgcccctcc aagggccgcg aggcgccgat cccgctggtg
    25561 gacggcgaag tgcatctctg gcaggtccag ctcccgatcc gcgacacggc cctgtgtcgc
    25621 gccgtggaga gcgccaccac cgccaacgaa cgggcccgcg ccggccgcct gcccttcgag
    25681 gacgaccgcc tgcgccagct gtacacccgc ggcatgctgc gcatcctgct gggacattac
    25741 ctgtccaagt cgccgaatgc cctgaacttc cccctcaacc cctgggacaa gccgctgctg
    25801 gaagaggacc cgggactcca cttcaacgtc tcccatgccc actccctggc gctcatcggc
    25861 gtcacccgct cggcgcccgt cggcgtggac ctggaagccg tgcgtcccct ggacgaccgg
    25921 gaagagcagt tccggcgcac cctgtgcagc gacgaactga gctggatcga ccgcttcagc
    25981 gagccgctcc ggcgtaacca cgagttcatg cgcctgcgca ccggcaagga agcggcgctc
    26041 aaggccctgg gaatgggcct ggcgcgccac ctcgacagct tcggtgtggt cccgcccccc
    26101 agcccggtcg ggccgggcga ggtcaacggt ctgcgtggac gctggacctt gcacttcccg
    26161 cccttgcccc agggcttcgc cggcgcggtg gcgatcgcca ccgacagcgc cggcccgcta
    26221 cgcgtgttcc gcgtcggctg aggcggccgg acggctcacc gcgtcacgat gtcccgatgc
    26281 aggggcgcca gacgcgccaa cagcttgttc tgtgcccgga acggcacccc ctgtgcgtcc
    26341 atcgcctgct ggaggtcctc caccagggca ttgaagtgcc cctgctggat gcccaggcca
    26401 gcgtgcaggg ggcccatcgg cgccccttga taggtgcatg gcccatccag caccgcgcag
    26461 atctgatcca ccagctgtgc cttgatgcgc gtctggtcgg catgttcgaa gaagggtcgg
    26521 gtccggccgt ccgccagcag attgaccatg aaggcgtcca tgaggctgac gaggcccggc
    26581 ttctcaccga acgcgcgata ggtggacaga tcggcctcgg catgggaggc gacgggcatc
    26641 agggcgagcg cgagggctgc gcagaagcta aaggtcttca tcgttcgatt cctttccgga
    26701 aaatcgcggg cgtcatcaga aacccagctg caccgacaga taggtcgcgc gctggttgtc
    26761 cttgatgacg atgttgccca ggttcgcgta ggccagcgtc accgccacgt tcttcaccgg
    26821 ctgccaggcc aggaaggcgt cataggcctt gtcctcgcgg gcgatgccca ggttgtcggg
    26881 cttggtccgg tactccacgc ccaccgccag atcgcggcgc aacaggtagg ccagcgagcc
    26941 ctcgaactgg gtcgtgtagc ggtccttgag atcgccgccg aagcccagga tgccgaactg
    27001 gttggccttg gtctcccgca gggtcaggtt ggccagcagg ctctggtcga ggaagagctt
    27061 ggtggccgct acgtagaagt cggtgccgtg gtccttcttc gcaccgatgg catgcagcac
    27121 cgccccctgg tcgttgcgct tgtgctgcag gccgatggcg atctgcggca gccaggaatc
    27181 ctgctccagc accgcgtcgc ccagcagccg cagcttggca ccgaagatct cctggttgaa
    27241 gctgaagccg ttgcccagcc ccagggcgcc gcccacgtcg cgggtatcga aggactggtg
    27301 cgcataggac agctccaggc gatcacggat gcccaccgcg atgccggaca cgcccagccc
    27361 gtagtcctgg gaacgcacct gggtatagaa ggcgctggcg ccaatctggt cgcgggtgcc
    27421 gtagccggcg atcaccgccc acggcgtgag gccgccgccg gccgcgcctt cgagctgcga
    27481 cacgccgcca gtgagcagca gcttgtcgct ggccgcgaag gccggcatgg ccgcccccca
    27541 ggccagcacg atgggcgcaa tccgaaccct gaatctcatg ttcatctccc tgatgaaaac
    27601 ggcggccgga agggccaccc ctgaccctcc tacgcagcac cgcacgcccc ggatgcagcc
    27661 cggcacgcga ttcgctatcc tcccgcgtcg tatgcactcg tccctgcccc atgaccgccc
    27721 gccgccttcc ggcctgcctc gcactcctcg cactcctcgg tctaaccgcc ctgcccgcgg
    27781 cctgggccgc cctgcctgac gaaatccagg tctatgccga cgacctcaac gcaccgggag
    27841 aattcggcct cgaagtgcac gtcaatacct cgccccgcgg ccagcgcgac gcgacctacg
    27901 cgggcgaggt gaagaacgac cacagcctgt tcgtcacgcc ggaactctcc tgggggctca
    27961 acgacacgat ggacgcgggc ctctacctgc ccaccgcccg cgacgcggcg accggcaacc
    28021 tctacgcggc cggcgccaag ggccgcttca agtggctccc gatccggggc gacgaactcc
    28081 agggcggcgg ttttgccggc ctcaacctgg agatcgcgcg gatcgcccgg cgcttcgagg
    28141 aataccgcag cgccgccgag ctgcgcgcca tgctcggctg gcgcacgccc gactggctgc
    28201 tcgccttcaa cccggtgctg agctgggatc tgtcggacgg ggctgaccgg cgcaccggct
    28261 acggtttcgc cttcaagggc acccaccgca tcggcgaagg cctcgccggc ggcttcgagt
    28321 actacaccga ccagggcaag gtgggcgagc gccagccctg gaacgcccag agcaacaagc
    28381 tcttcgccgt gctggacgtg gagcgccacg gctggggctt caatgtgggc gtcggcaagg
    28441 gcctctccac cgacaccgaa aagctgacgt tcaagggcat cctcgaattc gccttctgag
    28501 tgctgccggc ccccggcgcg cagcaccaaa agggggcgga gcgggacgcg cggcgccctt
    28561 ttccgcccca accggaggcg ctccgggaag ccgggcggcc ggcacgcaat cgcccggccg
    28621 cggcgcggtt ttcgggggcc gctgcaacgg ttgacggacc tgcgacgggt cgcgatactt
    28681 ctaagcttgt gccgggtccg gtcggcgcgt gggcgcaccg gaaagcatgg catgcaacag
    28741 ggcgttcgtt cgaaatcgac ccacccaaat caatcaaatc gagggaagaa acaatggctg
    28801 ctgggaaatc cacgatcatc tacacactca ccgacgaagc gccgctgctg gccacctgtg
    28861 ccttcctgcc cgttgtccgc accttcaccg cgcccgccgg cgtcgacatc gtcaagagcg
    28921 acatctccgt gtccgcccgc gtgatcgccg agttctccga catgctcccc gacgagctga
    28981 agatgcccaa caccctggcg gaactgggca ggatcaccct ccagcccgac gccaacatca
    29041 tcaagctgcc caacatctcg gcctccgtcg cccagctcaa ggcctgcatc aaggaactcc
    29101 aggaaaaggg ctaccccatc cccgacttcc cggaaaaccc caccaccgac accgaaaagg
    29161 ccatcaaggc gcgctacggc aagtgcctgg gttcctcggt gaacccggtc ctgcgcgaag
    29221 gcaactccga ccgccgcgct cccgccgccg tcaagaacta cgcccgcaag aacccccact
    29281 ccatgggtga atggaagcag tggtcccaga cccacgtgtc ccacatggag cacggcgact
    29341 tctaccatgg cgaaaagtcc ctgaccctcg acaaggcccg ccgcgtgcgc atggagctca
    29401 tcgccgtcgg cggcaagacc accgtcctca agcccgaagt caagctcctg gaaggcgaga
    29461 tcatcgactc catgttcatg agcaagacgg ccctgtgcaa cttctacgag cagcagctcg
    29521 aggactgccg cgaatccggc atcctcttct cgctgcacgt gaaggccacg atgatgaaga
    29581 tctcccaccc catcgtcttc ggccactgcg tcaagatcta ctacaaggac gccttcgaga
    29641 agcacgccaa gaccttcgaa gagctgggca tcaacgtcaa caacggcatg gtcgacctct
    29701 acgagaagat caaggccctg cccgaatcca agcgtgacga gatcatccgc gacctgcacg
    29761 cctgccagga acaccgcccc cgcctggcga tggtggattc tgccaagggc atcaccaact
    29821 tccactcccc caacgacgtg atcgtcgatg cctccatgcc cgccatgatc cgttccggcg
    29881 gcaagatgtg gggcgcggac ggcaagcagt acgactgcaa ggccgtgatg ccggaatcca
    29941 ccttcgcccg catctaccag gagatgatca acttctgcaa gtggcacggc aacttcgatc
    30001 ccaagaccat gggcaccgtg cccaacgtcg gtctgatggc ccagcaggcc gaagagtacg
    30061 gctcccacga caagaccttc gagatcgccg aggacggcat cgccaacatc gtcgacatcg
    30121 actccggcga agtgctcctc acccagaacg tggaagccgg cgacatctgg cgcatgtgcc
    30181 aggtcaagga cgccccgatc cgcgactggg tcaagctggc tgtcacccgc gcccgcaact
    30241 ccaacacccc cgccgtgttc tggctcgacc cctaccgtcc ccacgaagcc gagctgatca
    30301 agaaggtcga gacctacctc aaggagcacg acaccaccgg gctcgacatc aagatcctgt
    30361 cccaggtgcg cgccatgcgc ttcaccctgg agcgcgtcat ccgcggcctc gacaccatct
    30421 ccgtcaccgg caacatcctg cgcgactacc tgaccgacct gttccccatc atggaactgg
    30481 gcacctccgc caagatgctg tccatcgtcc cgctgatggc cggtggtggc atgtacgaaa
    30541 ccggcgccgg cggctccgcg cccaagcacg tgcagcagct cacccaggaa aaccacctgc
    30601 gctgggattc cctcggcgaa ttcctggccc tcgccgtgtc cctggaagag ctgggcatca
    30661 agacggacaa cgccaaggcc aagctcctcg ccaagaccct cgacgcggcc accggcaagc
    30721 tgctggacaa caacaagtcc ccgtcgcccc gcaccggcga gctggacaac cgcggcagcc
    30781 agttctacct gtccatgtac tgggcccagg aactcgccgc ccagaccgag gacgccgaac
    30841 tggccgccac cttcgcaccg ctcgccaagg ccctcaccga cagcgaagac cagatcgtgg
    30901 ccgagctgaa gatggtccag ggccgtccgg tggacatcgg cggctactac cgcgccgact
    30961 ccgagaagtg caaggccgtg atgcgtccca gccccacctt caacgccgcc ctgaaggccg
    31021 cccgcacctg atgtgacgcg ccctcagcgc atcgagcgct gacaagacaa cgccggcaga
    31081 cctggacacg ggtctgccgg cgttttcgtc tggggaattt cctgaccaag ccctggcgcc
    31141 ttgcctcatc gggcgccgcg cgccgtgccg gtctctgcgc agaagctcga cgctccgttt
    31201 gggcaggacg gtcgcgaaac cgtgcatcgc attcaggccc gggatccgcg tcacacgccg
    31261 acaggatcga tgcgtgccgg gcagcgccac cccccacggg gcacgaggcc ccctcagccg
    31321 cccagatcct cccgcacatc caca
//
LOCUS       DAOQMM010000032        30996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_394_length_30996_cov_44.027245, whole genome shotgun sequence.
ACCESSION   DAOQMM010000032 DAOQMM010000000
VERSION     DAOQMM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 30996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30996
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_394_length_30996_cov_44.027245"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1501
                     /gene="prpF"
                     /locus_tag="PLH85_07530"
     CDS             332..1501
                     /gene="prpF"
                     /locus_tag="PLH85_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HNB64450.1"
                     /translation="MTRFPPPVRPCRIPACLMRGGTSKGLFFLEQDLPDDPDLRDRLL
                     IRAVGSPDPYRQHMDGLGGATSSTSKVVLVGPSTHPGCDVNYRFGAVAVDRAVIDWSG
                     NCGNLSAAVAPFALHRGLVRVPRDGVAVVRIWQADIRKVIVAHIPVADGAVVEDGDFQ
                     LDGVAFPGAEIRLDFLDPAGDGEALFPTGHRRDTLSVAGLGNVDATLINAGNPTVFVH
                     ADTLGLTGTELQRDVNGQPKLLARAEALRAAAAVAMGLAPTADEATRNRPHTPKLAYV
                     AAPAPYVASDGRQIVAGEVDLLVRIFSMGPLHHAMTGTGAVAIAVAAAIPGTLVHEVA
                     RVNAGGRVCFGHPSGTLTAGADAQIDAAGNWVIRQVSMSRSARRLMDGQVFIPAV"
     gene            complement(1517..2572)
                     /locus_tag="PLH85_07535"
     CDS             complement(1517..2572)
                     /locus_tag="PLH85_07535"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNB64451.1"
                     /translation="MARSSSVSFEAVEQICFQLLSQGENPSFNLVYAELGNKGSSEIV
                     QRYIDQWRRETGAAFFRQRSVPGMPDGLVTATDRFMAEVWQMSLHHAEQLLESRRVEL
                     EQEREAIRAQLQLAHDQIGAQNAEAVRVAQQLETLERKAEACEITIGDLRAQNGDLMR
                     QREALETRVRLLEEAAETREQKFTAESERLAKLLQTDRDRYNASLKEEQERGAVEREH
                     LMKQIDVARQEKLAEITSMRQQLATREERETRQREQAEEARAALREAQSRARQMETSL
                     AAAEGRIERLQAQLKTASDAAHGKESAVAAAEARARAMEDVRIAETARAQRAEAALAE
                     TLAELGILRKKMAAGGA"
     gene            2694..3590
                     /locus_tag="PLH85_07540"
     CDS             2694..3590
                     /locus_tag="PLH85_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB64452.1"
                     /translation="MNFPAPPRAIAAIEREGILHPRAVEHLITGQPTRDGAGVTLNRL
                     ITPALQRRLDPFLMLDAFGSDSPDDYLAGFPDHPHRGFETITFMIEGRMRHRDSVGHE
                     GVLTSGGVQWMVAGRGVIHSELPEQQDGRMEGFQLWLNLAARDKLCPPWYRDIAAADL
                     PVYTTPDGVRVQIIAGRSHGIHGAVERPVTEPLYLDIRMTAGSHFAQPLPAGHNAFVC
                     VYRGQVCCGAADTEVAAGRMAILETNPDADGVLLTAKAPTRLILVAGRPLGEPIVQYG
                     PFVMNTEQEIRQTLEDFRAGRF"
     gene            3638..4042
                     /locus_tag="PLH85_07545"
     CDS             3638..4042
                     /locus_tag="PLH85_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB64453.1"
                     /translation="MTITVTRDTSGPMRHIVSVGRHRLTVDEPPDVGGEDAGPNPHDL
                     YDAALGACKALTLVWYARRKGIPLDAVEIEVERDASHERDGHYRLSTRLTLGGPLDEA
                     QRAQLLAVAARCPVHKLMTQVSTEIETTLKNG"
     gene            4193..4783
                     /locus_tag="PLH85_07550"
     CDS             4193..4783
                     /locus_tag="PLH85_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNB64454.1"
                     /translation="MAGHRAVLGVLQALKERLDVRMPPLTGGSPKALVLGSQTLAAGP
                     SGENLGIYLHRIAIDPFARNRYLAPAPPQRTPRPELPVNLHVLLIGWSVKNETEIGYL
                     AAAMQIIGGALTLDVSHLASADATWDDHDSVQVMPEEMSTEDLMRLWDSLPGDYRLST
                     PYLIKTLRLAPDLSADEGPLVRSVLIPAGTLPEDAP"
     gene            4780..5595
                     /locus_tag="PLH85_07555"
     CDS             4780..5595
                     /locus_tag="PLH85_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64455.1"
                     /translation="MILETVIVHGSDFIEWLDASGNAGGTVRRIAPARLRIELTKAPR
                     NLRIAHKPRATALLRTTDTPLPPAKEPARLALVVPGEATPAQRVPPAQNPYQIEGIVR
                     DPAGEFLPRRFSLNGGTAAGHQIRLFRSPLGSRYGSAGGVFGRLTFADDAPAAWALVE
                     LSVKPPLGATQTYTAQADAHGEFILPLDRLPAVAVDAVVKSYPATLTVLASATTDPNT
                     PFDPDELVACDVATGKDAGGQPEFAPSLSFTFTPGAISRVVSPEHSLLRLKSN"
     gene            5614..7782
                     /locus_tag="PLH85_07560"
     CDS             5614..7782
                     /locus_tag="PLH85_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNB64456.1"
                     /translation="MPEYLAPGVFIEETSFRQKSIEGVGTSVAALVGPTRSGPLRGTP
                     EALTSYAEFERTFGDAEDLKLGGSATLNLTSHAARAFFDNGGKQLFVVRVAAGGNDSD
                     ATSTGSSATAASVADSKGNLAFKARFPGAQGNLTLEILWRDTENLLASETVASAADGV
                     QYYLDARGVVEAAKVTGAITSDKFPVDIQAIVQKDGANLRIPDARATLVSKADPANPA
                     PVGANQITVLDPTKLPAGTQLRRVYARQPSAGALSADALAVLTLKAEADLSAFTKGDH
                     WGKALVLRGTLDKGGQTFTVKGDATLNPGITKDVSVPLAALASVPGAAGALVVQRSFD
                     LEVRNGGPDGEILYVYGSLDFTASGDRSLAKALPAAPERKEDALHSPVACTVKTLDGA
                     ALAATLYTLFTPAALFPPAGSYKDPRYLITLSGGTDGNVPGAGDYAGETNELLGSTGL
                     AALEAVEDVSIVMVPAAAAHADTHTAVLMEMLKHCRRMRYRVGLVDSREGMSLGEVRT
                     FRNDFDDSRLALYHPWVTIADPTGQRREITVPPAGFIAGVYARTDVDRGVHKAPANET
                     VLGALSFEQDINQFQQELLNPNGINCLRAFPGRGLRVWGGRTLSSDPEWKYVNVRRYF
                     LYLERSIEKSTQWAVFEPNGEALWANIRSSVEDFLFNEWKNGRLLGGAPKEAYFVRCD
                     RSTMTQNDLDNGRLVCVVGVAPLRPAEFVIFRIGQKTADA"
     gene            7799..8257
                     /locus_tag="PLH85_07565"
     CDS             7799..8257
                     /locus_tag="PLH85_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNB64457.1"
                     /translation="MAQMRETPYAAFNFLVELQPGQGKEVQAGFSEVSGLNAEVTVAE
                     YRAGNSTVNYVHKLPGIHKAGDVTLKRGVIGAQNLYEWLEASRSGRIKDAKREIVIKL
                     LSEDRADTVVSWKLRGAMPIKWTGPTLTAKGGGDVAIEELVLSVETVEQE"
     gene            8265..9887
                     /locus_tag="PLH85_07570"
     CDS             8265..9887
                     /locus_tag="PLH85_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64458.1"
                     /translation="MFLADQRIEIDARPAPPVPPVLEVVMLGHCPGPDDGSGASGAQW
                     QRVADDEALLADIAALGFSREVRRLKALRAGVDSAGRSVTPEPGDADLRALYRLPLPI
                     HALGQFRVLFPYSFDTPCRAVSTLAGDRAWLPRAVQDFFEDSTSLGREALKLWVIRVP
                     EAEGAHGFLPDAGAQLTDIGRLGAFERALLPPRAGLIVLPDLERLQVPALLPDIPRMQ
                     LDNPVPAFLPVGTAVDDGHRERRRGDEIPHATEPLPPEAVIEPLIRTLARLRPDMQCL
                     LALPLDADAGQEVPVPAPAWTAYLTRTVGSALKEGAASLDAASALRHLQWIYPYLRGA
                     DRPLGSPCGLVAGLQALTAQRAGPWRSIGERPFAGRDLPWPVLDQNQAAALRQAPGVT
                     VLLRRNGRIVVDDERLCVPCLPLPALRQLDAARRGQDHWYSAEVMRFMGWLQRQLRAL
                     GERLVFDADVHDPRTEMALRAFFTHLHARGALRGARPEDAYRLAIRSDGDSTLIADIE
                     IAPAFPLDLIRITFLQDRHNADARVQMEALDG"
     gene            9880..10371
                     /locus_tag="PLH85_07575"
     CDS             9880..10371
                     /locus_tag="PLH85_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNB64459.1"
                     /translation="MADSAPVPAEFIPFRFRVALFADEGGSQLVCRGAFSEVSGLEAS
                     VAVKNLKEGGRNWGEVQLSGPTTFSPVVLKRGFTEVGDLWKWFDAMNRQANYALRYTG
                     LIEVFHPSRAFTDGPTVKWWLHQVLPVKFKGPDLSATASQVAIEELHLAHEGFTLEAG
                     GAP"
     gene            10368..11066
                     /locus_tag="PLH85_07580"
     CDS             10368..11066
                     /locus_tag="PLH85_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64460.1"
                     /translation="MSPAGAPPSFAKAVLIPMNGDQVETDPAKHIPVQFNPSTLRVTL
                     ANTLHADSGGGRRGRRPAAQYVEKSESSLAVELVFDTSVAQQGTPANSDVRALTGRIA
                     ETFMKPVDPDSARPGAPKRCRFQWGSFKFTGMLASYGETLDFFAPEGIPLRATLALTF
                     KEDRYQFDRDESVRAAARAQPAFAPGGSDVSADRATQAAGGDPRDWRGVADLNALENP
                     RFTPAGGVRIPGRD"
     gene            11075..11224
                     /locus_tag="PLH85_07585"
     CDS             11075..11224
                     /locus_tag="PLH85_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64461.1"
                     /translation="MPVVIEELSAEITPDPHESPAQPAEDAAERAVLDLIELAREREA
                     RLAVD"
     gene            11211..12257
                     /locus_tag="PLH85_07590"
     CDS             11211..12257
                     /locus_tag="PLH85_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HNB64462.1"
                     /translation="MPSIDAPLLNVRPRFRVDGQDRPNLGEAVLALDLRLPRSGMAGA
                     EVRVLNWSGSGSAATFVFNDIRLGQRLELFLGEGANATAAFSGDITALEERYGDGAPQ
                     LVLLAEDGLHRLARRRTSRAFEDQSLDDVIRRIASDAGLQADVQAGSDQATWLQNNES
                     DLAFLLRQLEPRDIGLRLQDGRLRARDDEADPRPARLTVGANAEKIRLIADLNHQVSR
                     VQARGYDLAAATDLSGDASSLVPAPAGVSGVATLGSLGWNTVSTRPHPAPRTAGEAND
                     LARKGLRQSARRFVHGEIVCRDTPDLRGGREVELVDASPRLAGRYRVMDCHHRFDASA
                     GLRTHLRVERPDLS"
     gene            12267..12905
                     /locus_tag="PLH85_07595"
     CDS             12267..12905
                     /locus_tag="PLH85_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNB64463.1"
                     /translation="MSPHSLGPLHELHLASVLDNSDPDNRGRLRIRLAATGLETWAGV
                     IVASAGNGYGVSLLPRVGEQVVIAFVSPDLPLVIGALWAGGSSQPGDAAPVDQRYLVQ
                     TPAGFKLLMDDQAQRVRIETPAGYHFTVTDQGSGTVTIEKGAEKIEMASGGITLTTGS
                     KVTVQASSVEVSAGSVQVNAGMSKFSGVVQCDTLISNSVVSSSYTPGAGNIW"
     gene            12899..14521
                     /locus_tag="PLH85_07600"
     CDS             12899..14521
                     /locus_tag="PLH85_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64464.1"
                     /translation="MVNVPVAARPPSVHHTPRLLPTWFAGGADEGMSLHRYLDDQFVP
                     RFLQDAQAGRLTATRSQGWYQSDRVGRYKDAPLLRLPMHRAFYIACCEVSCDTFGLPA
                     FDPVKVRSAGFVVRRGLPGGAVQRWMIREGEALGWQGGAGDGGEPDEYRRLLHHGLLK
                     PRFPEPPYSGEETHPLHPALVKARDASGHVRNRTLLWGYLPLGGTYREQGGVEIPTDT
                     DTALGQEIAWPCGSRGARAWQAQDSRPVQLGYASQGFRELLSVLLRYRITDATQPDNE
                     ALRALLGQIHFYPAPSDRFAAPPPTTRGTSLLEWLDRSEAAVLDWLTRLAAGSATLTS
                     APLPSQSGLDAAGKPLSVPVVLDVYLSQTQAALLRQLVPARGRRAMARNDDGIAFPRY
                     PQGEDDLFHAVPFVRWEDDCGCERTVWGRPSLPFRVASPLDPDFQRPQAILLPRLDDL
                     KRGAARGIALMAPKSLADKLLGIAPKMPPAEGGPGNACGLCWVFSLSIPIVTICAMII
                     LMILINLLNLIFGWLPWALLALPRLCGKLLKD"
     gene            14525..14917
                     /locus_tag="PLH85_07605"
     CDS             14525..14917
                     /locus_tag="PLH85_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNB64465.1"
                     /translation="MASSAPRLLSFPLLDGVAPDGRLAWADGDKSIREVMLNILLTRP
                     GERLLRPDFGAGLRDFIHHPNNETTRALVADAVQRALVRWEPRVSIEEVRVVPDPQRL
                     SHINLSIRYRLRQDGRRDSLDLAIELGA"
     gene            14928..17105
                     /locus_tag="PLH85_07610"
     CDS             14928..17105
                     /locus_tag="PLH85_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNB64466.1"
                     /translation="MPLPLPDLDDRRFDDLVAELQQRLQRQVPELTALAPGDPAFALV
                     DLFAWLTETILYRANRIPDRQRLAFLNLLALPLRPARPARGVLCIDPSGGDGRSLAPL
                     VRTETALAAGQIALTTVGELQPLPLELHVLVKRTLDAATLSAEGISLAQLRQQYGVEP
                     AAFRPVRLTPPADTVTTAGSLDDSLYLAFAVPKALVRKADAVRRLLAGVVLNLALAPA
                     DDLDGELAASLSPRRLDWDVAVWRDPANHPESVVWLPLETVADSSRGGRQAGVVRLRL
                     PRDGSFLVVPSAIDPQFAGMGDTPPEAPADLAAGQLQCWLRLRSADGDGIELGYLGVN
                     GVDVLAQGVARDVMLGIGTGRPEQSLALPDAAVDTSDVRIDVESAGRFEPWQQVDHFG
                     AAGPDDAVYLVDGDSGSVRFGDGLRGRRPSDGLRIRASYYRFGGGTAGNLAPGAVQSV
                     AVGGGRLKPRQEWPLRGGVDAETVAEAEQRIPAFLAHRERAVTAEDFAVLARDNPLRP
                     IARADAAAGFLPGASLTAVRRNVPGVISVFVLPPAEPAFAAFPRPTAGTLRDVFEYLS
                     PRTLLGTELYVLSLQYQPVSVAVSVEVVDPALEQQTFRAVEQALLAYLWPLPPHGVRG
                     AGWPRGRAVDINELRTQASRVDGIEAVNGLRLFYQDLASGLWRELTGRLSLPLTDYQL
                     PELMAVAVQSGEATPAPPRAFAPGTSTGGSTRAVPVPVIPDVC"
     gene            17109..19505
                     /locus_tag="PLH85_07615"
     CDS             17109..19505
                     /locus_tag="PLH85_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64467.1"
                     /translation="MDSNGTRFLLLDRAADFRNAGGSCAWDADARAFTLVRHDEPRLP
                     RLAAADALQAWADAGPWILDDYGQIARLSADRRSIEAAVGWPDAPWNPLRAHRDPTAP
                     LAADLEALTLDPVDAPTACAFTDLHLGGSGLLAASWMADDGTRAGVTLVHLRARWQRH
                     LEIASPEAPAFRPVRLWVDASDRVWVASDTHLTLTIGAPLDPPYVPRPDRFEPVSVEP
                     SPPRVRWAQALPPLRRINAIAVVATRLLILAEASGSTDGAPQQILLVRALSDDPTAAL
                     TALPLPTGLPFASDIAALDDGRAILLPPLETGVTRAQRRDCPVITLDDPPALAAERWP
                     RRSEAAVRFVRHRDNQVRHLANDGVVRLFRLAQARFLPHGRALLTPVFDAGTPDTVWD
                     RLILDACIPAGCSVMVEAQAADTPGGFADTFHRQPLPLAFTTPDIPFATPRGTLHEIL
                     LQRSSGAARELRGRYLRLRLTLGGDGRHSPAVYALRIWYPRFSWQAHYLPDHFHQQQP
                     ADPEPVPAGRAANGADLRERLFASFSGLLTPIEDRIAASEQLLDPLAVPAPWLANLAA
                     MLGTRLRPHWPETRQRRWLAALGRLQPHKGSYGALCQALDILTEGGVSRGEVVPVELF
                     RLRRTLATVLGLSMDDARHPLTLGTGQSGNSLVGDTLVLSEDHAVEILALFAPELARP
                     GTERQAVEHFFDRYARRLTVILHGPAQSLRGVIEEALPELVPAIVQWNVLVSEHPFVL
                     GLSPLLRIDTYLETQPPPKPVRLDTTRLGRGDLIYNPVALLPEQAGSLPPPESEGDRA
                     "
     gene            19502..20437
                     /locus_tag="PLH85_07620"
     CDS             19502..20437
                     /locus_tag="PLH85_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64468.1"
                     /translation="MSQSDDNAATDSSLPDDGVFSEPLRRVSFEPGMLLGIEATRAEQ
                     AYHRRRHTRHAYWLHGAGTVAGLRVVMDSTPPKTAGKPWRVRLIITPGIGVDGLGREV
                     SVHEPYAIDLGDWLTAQHADSTAWGALERDGLDTAGTTLYLRITMRYQDVPSGLQPVM
                     ATAINAGTDPVGPSRIQDTVLYELTPWRPAIGDVPERPFTAHDDLSAYDDIADRLADH
                     ERAAIAAADAAGRRQLQLGARLLFALSDDNTALATRESLAVTAAELARTLLATVTIAL
                     DPGGQLVIDPARIVVDNLARPFLFNASTLARLLRQ"
     gene            20445..23057
                     /locus_tag="PLH85_07625"
     CDS             20445..23057
                     /locus_tag="PLH85_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HNB64469.1"
                     /translation="MANTLQFQPYAPGTTATAGGARLGSIDPRLSRTNYFDGQLLKAT
                     DLTRDQIYLDERLLELGQAFGAGIAEGLDLDIADGRYVRVQPGLAIAPSGRVLKLSGR
                     VLEADLQNSALIASLNNGAYRQFPRGLYAVVLQHAEVASGVAEAYPTDLESRRQWKVS
                     EYAEGVELVLVPLPIGLPRGDALSLRATLAREILHASGSLMLPSDDAIALGLVAIEQA
                     VVAWYDKGLVRRRLRNPLAPAAQGEDLALHYVELLHDVLDARSHAGLGDGFPAAQYFR
                     MLPAHGPLPKAAIDPVNGTQIFFPKAYEVSIAPVRRSDLPRLLADSALLAPIDLERDA
                     DIDVMVLVPMDDASFALRARQLEADPVTGTLGKTGILAHLDVFALRLYAPPALGTEDR
                     RTWEQIWAAVAPSDVRYVRRPPRTAETNVSAVVLARGFAIPEEITLPQRDLLDSEHQL
                     DQALTDIQALQVELSQQRPHIQDLEAQLATASGDLTTARNQVDSLNATNTSLTDANAR
                     LTGDLANLKTTNDTLTGDLQSATSRADEADARATALQGTVAELNTTITQLQARIDENP
                     DQRVAQLTTDLAAAQAAADTARQQLADTTSQLNAQRTAAEAATNQATAAQARADDLQN
                     QLNSANGRVASTESDLAGARDALNASNARLSATSAALDAANNRIGGLQNDVTTAQNAL
                     TVARGDADQLRGQVAGLNVELSNQAQIQAGLRNQIAADQATQAQLTAQINQQASQINA
                     LNNQIQLLQRQLADLRANPNASGVTLADVARVRGAQMDLGLKLDDQLRGRPSEVLNAA
                     YDLFLLIGVEYDEVLWPSLQDLARQPDRLPKLRDELVQLLTVQRMTVPKAMVQAGQNA
                     GLSPNLINQWANQR"
     gene            23085..24773
                     /locus_tag="PLH85_07630"
     CDS             23085..24773
                     /locus_tag="PLH85_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64470.1"
                     /translation="MTASIHVESPQAGPDDPRRLNLFPGLALPEQAFRKLQDYTDDRM
                     APLAGRLQPGILAGFEVSLEEAARAPRLRFRPGRGVTGAGQLVRLYTPLVTAWSDLLT
                     LAERDRGNAPLKDGLYLLTLRSVVEEVDALDTRSPAIRTEADPTRERRLEVVTLPGLV
                     ELSRSPRLLSLPQQETVNRLSVGFLDASPFTLAVGAVPVAMVKVADRQLAWLESVGGR
                     FMAEADAPHRTFLAHTLSVLQAGLQPLGSDASPAGFLLTERLGLSYLPAAGALPPALL
                     RNPADHPPQLDFNPSDLGIDLIPVPANTVEGIIAEELSRGYVDLLHPRRDRLRLMLAI
                     PDLDYRPDLLDLPLRDVRAEATLCERFDTAEAARQAWRNQWQKVFGNLAPVELLDTLL
                     QGTQPRLPGDVADQEPVAAELARPERSATRARVEEVRPPVSPDAFRGARRSLLVDAFS
                     AGKMADDPVFWLDDHPPRYEAVPAALPETDTTDGLLRQRADLRAQIKDMQTRLETSYK
                     LLNELNDYLGLQRQHFDALTVSFSALAGGVAGDGSGLKVMRWTNAASYTPASTK"
     gene            24789..26687
                     /locus_tag="PLH85_07635"
     CDS             24789..26687
                     /locus_tag="PLH85_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64471.1"
                     /translation="MNLLNKSGVFDNIADLKLQVGVKPSAALPQVEKLSTQQAFLTLE
                     PQTQAIAAFAVAAQAVESSAASAAARPADPIAIGTIAQTTQSLGTIRQFDVVQNYYST
                     ALENLTALRRIAIALGEDGKPTLDTTTTLLARVRRFLIDQLGSEDALNARLGQHGIAA
                     FSNLFTTLPPKIGATFTDADSKSATHNAHSALFHHGAVITDDIRGIEALRAVLVALRQ
                     DHQNQLAALQDQLATVETQLPEAAARLDTLNHTRLEALDDYAAAQHTLAEHWDAVAQA
                     YAERRRVIDSHRGLFYVKQRETPVTVTLPDPQDLRFTSPDDIVPGVPDREIPLADDLA
                     PFMEVVLDIPVADWAVLSGLAHLLPSRPRLELMVATRLQKVALRQKIGLPVATARLQG
                     LAFQNRGLLDDIALRPFAASAMLDLQRQGHAILALDDLLASPVPLLREPARVLHRRLD
                     AAAGGLLDLLRPIAPSLRLAWAAAAEADTLPVERPERWPGIAQAEAADLNGLRTFGEL
                     VAWWFRQLDSDASAASRTAMRNFVRACVMLATGDNPDEILQGHVSSPPRRFRIGEVMR
                     LELNREPRPGALLQLLDDAQRLVGTLRVDDHDNGQTVASIVRVLDDSLGVNRPLTTAF
                     RVTGSRNP"
     gene            26703..28346
                     /locus_tag="PLH85_07640"
     CDS             26703..28346
                     /locus_tag="PLH85_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64472.1"
                     /translation="MDRASATVTIARLNLRGARDDVARARTPLLAALDRAPWPDPGPD
                     TLLFVRHLRLRGSATDIARRAADLVRELTPRAEDGWSRAADQADAVRFRSRADMSACL
                     LADLLDGRAADRWFWARRAELFERPVPDAIVRILEEAPFELPELLDAGSARARPLAAI
                     ELLPRLMARLDAPATERLLGALRAATGWSLRFAAPEATAMAPAPGLPTALLATATRQA
                     HALRLAPAAPAARLVAVLLAWRHVPAHLGTPDADVLLTRLTHLVTQDGARSAPQQPMP
                     IARDATVPAPADTPSPHPAPLPTAAPVEARTTGAHPGRTTTTSDASREPASDAPAAEC
                     AAAALHSPRAAAPEDGFVTSCGGLLFLLNVLALTPVQSLLGDRNLPGCGWRWLARLAA
                     TLGLEPDPPLAAFLATEAALASPDALAVLPPLAEEARLEAFAAARYGADLWRADAFRL
                     PARIVATPSHVDAHFRLADARLDLRRAGLDLNPGWLPWLGRVVTFHFDDGPRALPAPM
                     GKPEDFTMGRDHSPALSPAGPPDTGPAAPAAAKKPGERP"
     gene            28343..30310
                     /locus_tag="PLH85_07645"
     CDS             28343..30310
                     /locus_tag="PLH85_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB64473.1"
                     /translation="MNAPLASARALDGAVLNRAFVRAGLARFGLTRLAAPSGLFAELA
                     ADAGLHCLLDSSPDDWAAACAALAARAPSLGGAWGALLTDYRLQLPEWFALALAAEVE
                     ADHAVGRALAELQAPDADGRPALHLLDALAATLFDAALPPLAWPHHALVQRGLASLSG
                     DLPLVRTSLRVDPRLAALLAGDRRPWADFTPLPPDTLTHVLPATLLTELDAAAHLVQQ
                     GAARLVVLRGSHGAGRAAAARLGAALGLLPVTIPPDPWRSTPIFAAACRYGGWLPVFL
                     TDLGPGDTFSLPTGSLEQTPQVLVTGQDGSVDTPDAIELDIPPLERHERHRAWQAVLG
                     PDAPPVLADHARLDGPAIHTLAQAVGRDAARRHEMPALAHLRRARSTHGAERLRQLAQ
                     PVHRDVGPDGLVLPAETARQFEHLVRRCRMRERLWDGLGPSLASPAPGVRALFAGESG
                     AGKTLAASRLATELGAPLYRVDLAAILNKYVGETEKNLGRLLDEAATLDALLLMDEAD
                     ALFGRRSEGKDGGERYANMMTNFLLTRIESHPGIVILTTNARNRLDAAFTRRFDTIIE
                     FPLPGVAERLRLWHAHLGARSPGEAACGLLATYSDLPGGHIRNAVLYAVALSPRPPGT
                     PVEITLLVAGLAEEYRKLGRTPPPQLAQIGE"
     gene            30314..>30996
                     /locus_tag="PLH85_07650"
     CDS             30314..>30996
                     /locus_tag="PLH85_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNB64474.1"
                     /translation="MASLTPVRRAAKRPDLARAPAPKDDKRPLQRAATPAKSPSPPAY
                     LQAKLAISHPSDHSEQEADRVAAQVARSPRPGEPETPQATPAARLQRASLQPGVEADD
                     VAPVPAARHLHRSPAPAQTPPTVHRKAAPDALAGQPASADTEARIEARRGGGAPLPDA
                     ARRDMEARFNQDFAAVRIHTDADAADLCGETRARAFTVGSDIFFAPGEFAPERGPGRE
                     LLAHELTHVV"
BASE COUNT         4634 a        12447 c         9412 g         4503 t
ORIGIN      
        1 ggcagcggaa agcacgccat gcccgaccgc tggttcgacc cgtcacccca cacccacgtc
       61 gccctggacg atgcgctgga acagggccga ctgttctgca acatgctggc cgacaatctt
      121 gcacggcgtg cgtgatgaag caccgctggt cagcctgggt cgtggcttcg cgcacatggc
      181 acacggccgt gcgacccaca atgagcctgc gctgcacgcg aatctttctt tctgaaaatt
      241 actgttttca gaactttaat catgccatcc gtacggtgcg atccgtcccg cgatgaaccc
      301 caccacaccc tcgccagcag ctgattccac agtgactcgt ttcccgccgc ctgtccgtcc
      361 ctgccgtatc cctgcctgcc tgatgcgtgg cggcaccagc aagggcctgt tcttcctcga
      421 gcaggatctg cccgatgatc cagacctgcg tgaccgcctg ctgatccgcg cggtcggcag
      481 ccccgacccc tatcgccagc acatggacgg cctcggcgga gccacctcaa gcaccagcaa
      541 ggttgtcctt gtcgggccct ccactcaccc ggggtgcgac gtgaactacc gcttcggcgc
      601 cgtggcggtg gatcgggccg tcatcgactg gtccggcaac tgcggcaatc tgagtgccgc
      661 cgtggcgcct tttgccctgc accggggcct ggttcgcgtt ccccgggacg gcgtggcggt
      721 cgtgcgcatc tggcaggcag acatccgcaa ggtgatcgtt gcccacatcc cggtggccga
      781 tggcgcagtg gtcgaagacg gagatttcca gctcgacggc gtggcatttc caggcgccga
      841 gatccggctc gacttcctcg atccggccgg cgatggcgag gccctgttcc ccaccggcca
      901 tcgacgggac acgctgtcgg tagccggcct cggaaacgtg gatgccaccc tcatcaatgc
      961 aggcaatccg acggtcttcg tgcacgcgga cacgctcgga ctgacgggca cggaacttca
     1021 gcgggacgtg aacgggcagc ccaagctgct ggcccgcgcg gaggccctcc gcgccgccgc
     1081 ggcggtcgcc atgggcctcg ccccgaccgc tgatgaggcc acccgcaatc gaccgcatac
     1141 gcccaagctg gcctatgtcg cggcacccgc gccctacgtg gcatcggacg gccgtcagat
     1201 cgtcgctggc gaggtggatc tgctcgtacg gattttctcg atggggccgc tgcatcacgc
     1261 catgacagga acaggcgcgg ttgccattgc cgtggcggcg gcgatcccgg gcacgctggt
     1321 ccatgaggtg gcgcgcgtca atgccggggg gcgcgtgtgc ttcggccatc cctcgggcac
     1381 gctgacggca ggggccgacg cacagatcga cgcggccggc aactgggtca tccgtcaggt
     1441 gagcatgtcg cgcagcgcac gacgtctgat ggacggccag gtattcattc ctgccgttta
     1501 gccgtcaagc gggcgctcag gcgccgccgg cagccatctt cttgcgcagg atgcccagtt
     1561 ccgccagcgt ctcggccagc gcggcctcgg cgcgctgggc acgggccgtt tcggcgattc
     1621 gcacgtcctc catcgcgcgg gcgcgcgctt ccgccgcggc cacggccgat tccttgccat
     1681 gggctgcgtc gctggcagtc ttcaattggg cctgcaagcg ctcgatgcgt ccttccgcag
     1741 cggccaggga agtctccatc tgacgggcgc gggactgggc ctcgcgcagc gctgcacggg
     1801 cctcttcagc ctgctcgcgc tgacgcgtct cacgctcctc gcgggtggcg agctgttgcc
     1861 gcatggaggt gatctccgcc agtttctcct ggcgggccac atcaatctgt ttcatcaggt
     1921 gctcgcgctc cacggcacct cgctcctgct cttccttgag cgacgcgttg taacggtccc
     1981 ggtcggtctg gaggagcttg gcgagccgtt cggattccgc ggtgaatttc tgctcccgcg
     2041 tctcggcggc ttcttcgagg aggcgcacgc gggtctcgag ggcttcgcgt tgccgcatca
     2101 ggtcaccgtt ctgcgcacgt agatcgccga tggtgatctc gcacgcttcc gccttgcgct
     2161 ccagcgtctc cagttgctgg gccacgcgaa cggcttcggc gttctgggca ccgatctggt
     2221 catgcgccag ctgcaactgg gcacggatcg cctcacgctc ctgctcgagt tccaccctgc
     2281 gggattccag cagctgttcg gcgtgatgca gggacatctg ccagacctct gccatgaagc
     2341 ggtcggtggc cgtcaccagt ccgtcgggca tgcctggaac gctgcgctga cggaagaagg
     2401 ccgcaccggt ttcccgccgc cactggtcga tataccgctg gacgatctcg ctggatccct
     2461 tgttgcccag ctcggcgtag acgaggttga aggacgggtt ctcgccctgg gacagaagct
     2521 gaaagcagat ctgctccacc gcttcgaaac tgacactgct gctgcgggcc atgtgtgttt
     2581 cccccatttt tccccggatg gtctagtctg aatccagggg gcacggtttc caaatattat
     2641 acgaccgtat aacgtcagat ttaatacggt atttttcacg gagccacata ttcatgaatt
     2701 tcccggcccc tccccgcgcc atcgcggcga tcgagcgtga aggcatcctc cacccccgcg
     2761 ccgtggagca tctgatcacc ggacaaccca cccgggatgg tgccggcgtc acgctgaacc
     2821 gcctcatcac tcctgccctg cagcgacgcc tggatccttt cctgatgctc gacgcctttg
     2881 gcagcgacag tccggacgat tacctggcgg gcttccccga tcatccccac cggggcttcg
     2941 aaacgatcac gttcatgatt gaaggacgca tgcgccatcg ggacagcgtc gggcacgaag
     3001 gcgtgctgac aagcggcggt gtccagtgga tggtggccgg gcgcggcgtg atccattccg
     3061 aattgcccga acagcaggac gggcgcatgg aaggctttca gctctggctc aacctggcgg
     3121 cccgggacaa gctgtgtccg ccgtggtacc gcgacattgc cgcggccgac ctgcccgtct
     3181 acaccacgcc ggacggtgtc cgcgtgcaga tcatcgcagg gcgcagccac ggcatccacg
     3241 gcgcggtgga acgtcccgtc accgagccgc tctacctgga catccggatg accgccggga
     3301 gccacttcgc gcagccgctg cccgccggcc ataacgcctt tgtctgcgtg tatcgcggac
     3361 aggtctgctg tggcgcggca gatacggagg tcgcagccgg gcgcatggcg atcctcgaaa
     3421 ccaaccccga tgcagacggt gtgttgctga ctgcaaaggc gccgacccgg ctcatcctcg
     3481 tcgcgggccg gccccttggc gaacccatcg tccagtacgg accattcgtc atgaacaccg
     3541 aacaggaaat ccgtcagacc cttgaggatt tccgcgccgg gcggttctga gtcgtccggt
     3601 cgcgcctcac cctccgtcct tccgggaaac ctacgctatg accatcaccg tcacccgcga
     3661 cacttcaggt cccatgcgcc atatcgtctc cgtcggccgt caccgcctga ccgtggacga
     3721 accgcccgac gttggtggcg aagatgctgg cccgaatccc catgacctgt acgacgccgc
     3781 cctcggcgcc tgcaaggccc tgaccctcgt gtggtacgcg cgacgcaagg gcatcccgct
     3841 cgacgctgtc gaaatcgaag tggagcggga cgccagccac gaacgcgacg gccattaccg
     3901 gttgagcacg aggctgacgc tcggcggtcc gctcgatgag gcccagcggg cccagctgct
     3961 ggcggtcgca gccaggtgcc cggtacacaa gctgatgacc caggtgagca ccgaaatcga
     4021 aacgaccttg aagaacggct gaatacgaca taaaaaagta acagtcacct tcctacgtcc
     4081 atggcatcgt agcggtgcga ggaaggctgt ggtcccctca cagcgttccg gcgtcctcca
     4141 atcggcagtg cgtgtttgcg gagcaatcac catgaggcag gcggagtagg tcatggcggg
     4201 tcatcgcgcg gtcctcggag tcctgcaggc gctcaaggag cgtcttgatg tgcgcatgcc
     4261 gcccctgacc ggtggcagcc cgaaggccct tgtccttggc tcccagaccc tcgcagcggg
     4321 cccctcgggt gaaaacctgg gcatctacct gcaccgcatc gccatcgacc cctttgcccg
     4381 caaccgctac ctcgcccccg ccccgcccca gcgcacgccc cgcccggaac tgcccgtcaa
     4441 tctgcacgtc ctgctgattg gctggagcgt caagaacgag accgaaatcg gctacctggc
     4501 cgcggccatg cagatcatcg gcggcgccct caccctggat gtctcccacc tggccagcgc
     4561 cgacgccacc tgggacgacc atgacagcgt gcaggtgatg cccgaagaga tgagcaccga
     4621 ggatctcatg cgcctctggg acagtctgcc cggtgactac cggctgtcca ccccctacct
     4681 catcaagacc ctgcgcctgg cgcccgatct gagcgccgac gagggcccgc tggtgcgcag
     4741 cgtactgatc cccgccggca cgctgcccga ggacgcgcca tgatcctcga aaccgtcatc
     4801 gtccacggca gcgacttcat cgaatggctg gatgcctccg gcaatgccgg cggcaccgtc
     4861 aggcggatcg ccccggcgcg cctgcgcatc gagctcacca aggcgccccg caacctgcgg
     4921 atcgcccaca agccccgcgc cacggccctc ctccgcacca ccgacacacc gcttccaccg
     4981 gccaaggagc ctgcccgact ggccctcgtc gtgcccggtg aggcgacacc cgcccagcgc
     5041 gtgccgccgg cccagaatcc ctaccagatc gaaggcattg tccgcgatcc ggccggcgaa
     5101 ttcctgcccc gccgcttcag cctgaacggc ggcacggccg ccgggcacca gatcaggttg
     5161 ttccgctccc cgctcggcag ccgctatggc agcgccggcg gcgtgttcgg gcgcctcacc
     5221 tttgccgacg acgcgccggc cgcgtgggcc ctggtcgagc tcagcgtcaa gcctcccctc
     5281 ggcgcgaccc agacctacac ggcccaggcc gatgcccacg gcgaattcat ccttcccctc
     5341 gaccgcctgc ctgcggtcgc cgtggacgcg gtggtcaaga gctaccccgc caccctcacc
     5401 gtgctggcat ccgcaacgac cgatccgaac acccccttcg atccggatga gctcgtggcc
     5461 tgcgacgtcg ccaccggcaa ggacgccggc ggtcagccgg agtttgcccc gtccctgtcc
     5521 ttcaccttca cgccgggcgc catcagccga gtggtcagtc cggaacacag cctgctgcgc
     5581 ctgaagtcga actaacccgc aaggagaccg accatgcccg agtatctcgc cccaggcgtc
     5641 ttcatcgagg agacgagttt tcgccagaaa tccatcgaag gggtcgggac cagcgtggcc
     5701 gcccttgtcg gtcccacgcg cagtggtccg cttcgcggca cacccgaagc gctcaccagc
     5761 tatgccgagt tcgaacgcac cttcggcgac gccgaggatc tgaaactcgg tggcagtgcc
     5821 accctcaacc tcacgtccca cgccgcccgc gcctttttcg acaacggtgg caagcagctc
     5881 ttcgtggtac gtgtggccgc cggcggcaac gacagcgacg cgaccagcac cggcagcagc
     5941 gccaccgccg cttcggtggc cgacagcaag ggcaatctcg cattcaaagc ccgattcccc
     6001 ggcgctcagg gcaacctgac cctcgagatc ctgtggcgcg acaccgaaaa cctgcttgcc
     6061 tccgagaccg tcgccagtgc agccgacggt gtccagtact acctcgatgc ccgcggcgtc
     6121 gttgaggccg ccaaggtcac cggcgccatc accagcgaca agttcccggt ggacatccag
     6181 gccatcgtcc agaaggacgg cgccaacctg cgcattcccg acgcccgcgc cacccttgtc
     6241 tccaaggccg accccgccaa cccggcgccc gtcggcgcca accagatcac ggtgctcgac
     6301 cccaccaagc tccccgccgg cacccagctc aggcgcgtgt atgcccgcca gccctccgcc
     6361 ggtgccctga gcgccgacgc gctggccgtc ctcaccctca aggccgaagc cgacctgtcg
     6421 gcgttcacca agggcgacca ctggggtaaa gccctggtcc tgcgtggcac cctcgacaag
     6481 ggcggccaga ccttcaccgt gaagggcgac gcgaccctca acccgggcat cacgaaggac
     6541 gtcagcgtgc ccctcgccgc cctcgccagc gtaccgggcg cggccggcgc cctggtggtc
     6601 cagcgcagct tcgatctgga agtgcgcaac ggcggcccgg acggcgagat cctctacgtc
     6661 tacggcagcc tggacttcac cgcctcgggc gaccggagcc tcgccaaggc cctgcctgcg
     6721 gcaccggaac gcaaggagga cgccctccac tcgccggtgg catgcaccgt caagaccctc
     6781 gacggcgcgg cgctcgcggc cacgctctac accctgttca ccccggcggc cctgtttccc
     6841 ccggccggca gctacaagga tccgcgctac ctcatcaccc tgtccggcgg gaccgacggc
     6901 aatgtgccgg gtgccggcga ctacgccggc gagaccaatg aactcctcgg cagcaccggc
     6961 ctcgccgccc tcgaagcggt cgaggacgtc tccatcgtca tggtcccggc cgccgccgcc
     7021 cacgccgaca cccacaccgc cgtgctcatg gagatgctca agcactgccg gcgcatgcgc
     7081 taccgggtcg gtctggtgga cagccgcgag ggcatgagcc tgggcgaagt gcgcaccttc
     7141 cgcaacgact tcgacgacag ccgcctggcc ctttaccacc cctgggtcac catcgccgat
     7201 cccaccggcc agcggcgcga gatcaccgta ccgcccgcag gattcattgc cggcgtctac
     7261 gcgcgcaccg acgtggaccg gggcgtccac aaggcccccg ccaacgagac cgtgctgggc
     7321 gccctgagct tcgagcagga catcaatcag ttccagcagg agctcctcaa ccccaacggc
     7381 atcaactgcc tgcgcgcctt ccccggccgc ggcctgcgcg tgtggggcgg acgcaccctg
     7441 tccagcgatc cggaatggaa atacgtcaat gtgcggcgct acttcctcta cctggaacgc
     7501 tccatcgaaa aatccaccca atgggcggtc ttcgagccca acggtgaagc cctgtgggcc
     7561 aacatccgca gctcggtcga ggacttcctg ttcaacgaat ggaagaacgg ccggctcctc
     7621 ggcggcgccc ccaaggaagc ctatttcgtc cgttgtgacc gctccaccat gacccagaac
     7681 gacctggaca acgggcggct cgtctgcgtg gtcggcgtcg ccccgttgcg tcccgccgaa
     7741 ttcgtgatct tccgcatcgg ccagaagacc gccgacgcct gacctggaga gctgaaccat
     7801 ggcccaaatg cgcgaaaccc cctacgccgc cttcaacttc ctcgtggagc tccagcccgg
     7861 ccagggcaag gaggtccagg ccggcttctc cgaggtctcc ggcctcaacg ccgaagtcac
     7921 cgtcgccgaa taccgcgccg gcaattcgac ggtcaattac gtgcacaagc tgcccggcat
     7981 ccacaaggcg ggcgacgtga ccctcaagcg cggcgtgatc ggcgcccaga acctctacga
     8041 atggctcgaa gccagccgca gcgggcgcat caaggacgcc aagcgcgaga tcgtcatcaa
     8101 gctcctctcc gaagaccggg ccgacaccgt cgtcagctgg aagctgcgcg gcgccatgcc
     8161 tatcaaatgg accggaccca ccctcaccgc caagggcggc ggcgacgtgg ccatcgagga
     8221 gctggtgctg tccgtggaga cggtggaaca ggagtaatcg gtccgtgttc ctcgccgacc
     8281 agcgcatcga gattgacgcc cgcccggcgc cccctgtgcc gccggtgctg gaggtggtca
     8341 tgctcggcca ttgccccggc ccggacgacg gcagcggcgc gtccggtgcc caatggcagc
     8401 gggtggccga cgacgaggcc ttgctcgccg acatcgccgc cctcggcttc agccgggagg
     8461 tgcgccggct caaggccctg cgcgcgggcg tggacagcgc cggacggtcc gtcacccccg
     8521 agcccggcga tgcggatctg cgcgcactct accgtctgcc gctgccgatc cacgcccttg
     8581 gccagttccg cgtgctcttc ccgtacagct tcgacacccc gtgtagggcg gtcagcaccc
     8641 tggccggcga ccgggcctgg ctgcctcggg ccgtacagga cttcttcgag gacagcacct
     8701 ccctcggccg cgaagccctc aagctgtggg tcatccgcgt ccccgaggcc gaaggcgccc
     8761 acggctttct ccccgacgcg ggtgcccagc tcaccgacat cggacgcctt ggcgccttcg
     8821 agcgggcgct gctccccccg cgggcggggc tgatcgtgct gccagatctt gaacgcctcc
     8881 aggtgcccgc cctcctcccg gacatcccgc gcatgcagct ggataacccg gtgccggcct
     8941 tccttcccgt gggcacggcc gtggacgacg gccaccggga acgtcgccgc ggcgacgaga
     9001 ttccccacgc caccgaaccg ctgccccccg aggccgtcat cgagccgctg atccgtaccc
     9061 tcgcccgcct gcgaccggac atgcagtgtc tgctcgccct gcccctggac gcggacgccg
     9121 gccaggaagt cccggtgccg gcccccgcct ggaccgccta cctcacgcgg acggtcggca
     9181 gcgccctcaa ggaaggcgct gccagtctcg acgccgcctc cgcactgcgt cacctgcagt
     9241 ggatctaccc ttatctgcgg ggtgccgatc gtccccttgg ctccccgtgc ggcctcgtcg
     9301 ccggattgca ggccctcacc gcccagcgcg ccgggccgtg gcgctccatc ggcgaacgtc
     9361 ccttcgccgg acgcgatctg ccctggccgg tgctggacca gaaccaagcc gccgccctgc
     9421 gtcaggcacc gggcgtcacc gtattgctgc gtcgcaacgg gcgcatcgtc gtggacgatg
     9481 aacgcctgtg cgttccctgc ctgccgctgc cggccctgcg ccagctcgac gccgcacggc
     9541 gcggtcagga tcactggtat tccgccgagg tcatgcgctt catggggtgg ctgcaacgcc
     9601 agttgcgggc gctgggcgag cgcctggtct tcgacgccga tgtgcatgat ccccgcaccg
     9661 agatggccct gcgtgccttc ttcacacacc ttcacgcccg tggagccctg cgcggcgcac
     9721 ggcccgaaga cgcctatcgc ctggccatcc gcagtgacgg cgacagcacg ctgatcgccg
     9781 acatcgagat cgcaccggcc tttccgctgg acctgatccg catcaccttc ctgcaggacc
     9841 ggcacaacgc cgacgcccgt gtgcagatgg aggccctcga tggctgacag cgcgcccgtt
     9901 ccggccgaat tcatcccctt ccgcttccgc gtcgccctct ttgcggacga aggcggcagc
     9961 cagctcgtgt gccgcggtgc cttcagcgaa gtcagcgggc tggaagccag cgtggcggtc
    10021 aagaatctca aggaaggtgg ccgcaactgg ggcgaagtgc agctctccgg gcccaccacc
    10081 ttctcgccgg tggtcctcaa acgtggcttc accgaagtgg gcgacctgtg gaagtggttc
    10141 gacgcgatga atcgccaggc caactacgcc ctgcgttata ccgggctgat cgaggtcttc
    10201 catccctccc gcgccttcac cgacggcccc accgtcaagt ggtggctgca ccaggtgctg
    10261 ccggtcaagt tcaagggccc ggacctctcg gccaccgcca gccaggtggc catcgaggaa
    10321 ctgcacctcg cccatgaagg ctttaccctc gaagcgggag gcgccccatg agccccgcag
    10381 gcgctccgcc cagcttcgcc aaggccgtgc tcatccccat gaacggcgac caggtcgaga
    10441 ccgatccggc caagcacatc ccggtgcagt tcaacccgtc caccctgcgt gtgaccctcg
    10501 ccaacaccct gcacgccgac agcggtggcg gccgtcgcgg gcgccgcccc gctgcccagt
    10561 acgtagagaa gagcgaatcc tccctggccg tcgaactggt cttcgacacc tcggtcgccc
    10621 agcagggcac gcccgccaat tccgacgtgc gggccctcac cggacgcatc gccgagacct
    10681 tcatgaagcc cgtcgatccc gactccgccc gtcctggcgc gcccaagcgc tgccgcttcc
    10741 agtggggcag cttcaaattc accggcatgc tcgcctccta cggtgaaacg ctggatttct
    10801 ttgccccgga gggcataccg ctgcgcgcca cgctggccct caccttcaag gaagaccgct
    10861 accagttcga ccgggacgaa tccgtgcgcg ccgccgcccg cgcccagccc gccttcgccc
    10921 cgggcggcag cgatgtcagc gcggaccggg ccacccaggc tgccggcggc gatccccgcg
    10981 actggcgcgg cgtcgccgac ctcaacgccc tcgaaaaccc ccgcttcacc ccggccggcg
    11041 gggtccgcat ccccggccgc gactaggagc cgccatgccc gtcgtcatcg aagaactctc
    11101 ggccgaaatc acccccgacc cccacgagtc cccggcccag cccgctgaag atgccgccga
    11161 gcgggccgtc ctcgacctga tcgaactcgc ccgcgaacgg gaggcccgcc ttgccgtcga
    11221 ttgacgcccc gctcctcaac gtccgccccc gcttccgggt ggacggccag gaccgtccca
    11281 acctgggcga ggccgtgctc gccctggacc tgcgcctgcc ccgcagtggc atggcgggtg
    11341 ccgaggtgcg cgtgctcaac tggagcggaa gcggcagcgc cgccacgttc gtcttcaacg
    11401 acatccggct ggggcaacgt ctggaactct tcctcggcga aggtgccaac gcgacggccg
    11461 ccttcagcgg cgacatcacc gccctcgaag agcgctatgg cgacggcgcg ccgcagctcg
    11521 tgctgctggc cgaggacggt ctgcaccgcc tggcccgccg ccgtaccagc cgcgctttcg
    11581 aagaccagag cctggacgat gtgatccgcc gcatcgccag cgacgccggc ctgcaggccg
    11641 acgtgcaggc cggcagcgat caagccacct ggctgcagaa caacgagagc gacctggcct
    11701 tcctgctgcg tcagctcgaa ccccgcgaca tcggcctgcg cctccaggac ggtcgcctgc
    11761 gggcccgtga tgacgaagcc gacccccgcc cggcgcgcct cacggtgggt gccaacgccg
    11821 agaagatccg cctgattgcc gatctcaacc atcaggtcag tcgcgtccaa gcccgggggt
    11881 acgatctggc cgctgccacc gacctcagcg gtgacgcgtc cagcctggtc cccgcgccgg
    11941 caggcgtgag tggcgtcgcc accctgggca gcctgggctg gaacacggtc tccacccggc
    12001 cccatcccgc cccgcgcacg gccggcgagg ccaacgacct ggcccgcaag ggactgcgtc
    12061 agagcgcccg ccgcttcgtc catggcgaga tcgtctgccg cgacaccccg gatctgcgcg
    12121 gtggtcgcga ggtcgaactg gtggacgcgt cgccccgtct ggccggccgc tatcgggtga
    12181 tggactgcca tcaccgcttc gacgcttccg ccggcctgcg cacccacctg cgcgtcgagc
    12241 ggcccgacct gagctgaggc gcccccatga gcccccacag cctcggcccc ctccacgaac
    12301 tccacctggc ctcggtgctg gacaacagcg acccggacaa ccgcggccgc ctgcgcatcc
    12361 gcctcgccgc cacgggcctg gaaacctggg ccggcgtcat cgtcgccagc gctggcaatg
    12421 gctatggcgt cagcctgctg ccgcgggtcg gggaacaggt cgtcatcgcc ttcgtgagcc
    12481 ccgacctgcc cctggtgatc ggcgccctgt gggccggcgg cagcagccag cccggcgacg
    12541 ccgcgccagt ggatcagcgc tacctcgtcc agacgcccgc cggcttcaag ctgctgatgg
    12601 acgatcaggc ccagcgcgta cgcatcgaga cgcccgccgg ctaccacttc accgtcacgg
    12661 atcagggcag cggcacggtc actatcgaaa agggtgcgga aaagatcgag atggcgtccg
    12721 gcggcatcac cctcaccacc ggctcaaagg tcaccgtgca ggcctcctcg gtcgaggtct
    12781 cggccggctc cgtgcaggtt aacgcgggca tgagcaaatt ctccggcgtg gtccagtgcg
    12841 acaccctgat cagcaacagt gtggtgtcgt ccagctacac accgggcgcg gggaacatat
    12901 ggtgaacgtc cccgtggcgg cccggccgcc ttccgtccat cacacgccgc gcctgctgcc
    12961 gacctggttc gcagggggcg cggacgaggg catgagcctg caccgctacc tggacgacca
    13021 gttcgtaccg cgcttcctgc aggacgccca ggccggccgt ctcacggcca cccgcagtca
    13081 gggctggtac cagtccgacc gggtcgggcg ttacaaggac gcgcccctgc tgcgcctgcc
    13141 catgcaccgg gccttctaca tcgcgtgctg cgaagtgagc tgcgacacct tcggcctgcc
    13201 ggccttcgat ccggtcaagg tccgctccgc cggcttcgtc gtgcgccgcg gcctgcccgg
    13261 cggcgccgtc cagcgctgga tgatccgcga aggcgaggcc ctcggctggc aaggcggggc
    13321 cggcgacggc ggcgaaccgg atgagtaccg gcgtctgctt catcacggcc tgctcaagcc
    13381 gcgcttcccc gaaccgccct attccggcga agagacccac cccctccacc cggccctcgt
    13441 caaggcccgc gacgcatccg gccacgtgcg caaccgcacc ctcctgtggg gctatctgcc
    13501 cctcggcggc acctatcgcg agcagggcgg cgtggagatc ccgactgaca cggacacggc
    13561 cctcggtcag gagatcgcgt ggccctgcgg cagccggggc gcccgcgcct ggcaggcgca
    13621 ggattcccgc ccggttcagc tcggctatgc cagccagggc ttccgcgaac tcctcagcgt
    13681 cctcctgcgc tatcgcatca ccgacgccac ccagcccgac aacgaggccc tgcgcgccct
    13741 cctcggccag atccatttct acccggcccc gtccgaccgc ttcgccgcgc cgccccccac
    13801 gacccgcggc acctccctgc tcgaatggct cgaccgcagc gaagccgcgg tgctggactg
    13861 gctcacccgc cttgccgccg gcagtgcgac cctcacgagc gccccgctcc ccagtcagag
    13921 cggcctcgac gcggccggca agccgctcag cgtccccgtc gttctggatg tctatctctc
    13981 ccagacccag gccgccctgc tgcgtcaact cgtgccggcc cgggggcgcc gcgccatggc
    14041 ccgcaacgac gacggcatcg cctttccgcg ctacccccag ggtgaggacg acctcttcca
    14101 tgccgtaccc ttcgtccgct gggaggatga ctgcggctgc gagcgcaccg tgtggggccg
    14161 ccccagcctg cccttccgcg tcgcctcccc cctcgatccg gacttccaac gtccgcaggc
    14221 catcctgctg ccgcgcctgg acgacctcaa gcgcggcgcg gcccgcggca ttgccctgat
    14281 ggcgcccaag tccctcgccg acaagctcct cggcattgcc cccaagatgc cacccgccga
    14341 gggcggtccc ggcaacgcct gcggactgtg ctgggtcttc agcctgagca tccccattgt
    14401 caccatttgc gcgatgatca tcctgatgat cctcatcaac ctgctcaacc tgatcttcgg
    14461 ctggctgccc tgggccctgc tggccctgcc ccgcctgtgc ggcaagctcc tcaaggactg
    14521 agccatggcc tcctccgcgc cccgcctcct cagctttccc ctcctcgacg gcgtcgcgcc
    14581 cgatggccgt ctcgcgtggg ccgacgggga caagagcatc cgcgaggtga tgctcaacat
    14641 cctcctcacg cgccccggcg aacgcctgct gcgcccggac ttcggcgccg gcctgcggga
    14701 cttcatccac caccccaaca acgagacgac ccgcgccctc gtcgccgacg cggtgcagcg
    14761 tgccctcgtc cgctgggaac cgcgcgtgag catcgaggag gtccgcgtgg tgcccgaccc
    14821 gcaacgcctg tcccacatca acctgtccat ccgctaccgc ctgcgccagg acggccgccg
    14881 ggacagcctt gatctggcca tcgaactcgg tgcctaggac gaccatcatg cccctgcccc
    14941 ttcccgatct ggacgaccgc cgcttcgacg acctcgtcgc cgaactgcag cagcggctcc
    15001 agcgccaggt gcccgaactt accgccctgg cccccggcga cccggccttt gcgctggtgg
    15061 acctcttcgc ctggctcacc gagaccatcc tctaccgcgc caaccgcatc cccgaccgcc
    15121 agcgcctcgc tttcctcaac ctcctggccc tgcccctgcg ccccgcgcgc cccgcccgcg
    15181 gcgtgctgtg catcgatcct tccggcggcg atggccgcag cctggcgccg ctggtgcgca
    15241 ccgagacggc cctcgccgcg ggccagatcg ccctcaccac cgtgggcgaa ctccagcccc
    15301 tgcccctgga actgcacgtc ctcgtcaaac gcacgctgga cgccgccacc ctcagcgccg
    15361 aaggcatctc cctggcccag ctgcgccagc aatacggtgt cgaaccggcc gccttccgcc
    15421 cggtgcgcct cacgccgccg gccgacacgg tcaccacggc cggcagcctg gacgacagcc
    15481 tctacctcgc cttcgccgtg cccaaggcgc tggtgcgcaa ggccgacgcc gtccgccgcc
    15541 tgcttgccgg cgtggtcctc aacctggccc tggcgcccgc cgacgacctg gacggcgagc
    15601 tggccgccag cctgtcaccc cgtcgcctcg actgggacgt ggcagtctgg cgcgatcccg
    15661 cgaaccatcc cgagagcgtc gtctggctgc ccctcgagac cgtcgccgac tcctcccgcg
    15721 gcggtcgcca ggccggcgtg gtgcgcctgc gcctgccccg cgacggcagc ttcctggtcg
    15781 ttccgagcgc catcgacccc cagttcgcgg gcatgggcga cacgcccccg gaagcccccg
    15841 ccgacctggc cgccggccag ctccagtgct ggctgcgcct gcgcagcgcc gacggcgacg
    15901 gcatcgagct gggctacctg ggcgtcaatg gcgtggacgt cctcgcccag ggcgtcgccc
    15961 gcgacgtgat gctgggtatc ggcaccggcc ggcccgaaca gagtcttgcc ctgccggacg
    16021 ccgccgtgga cacgtccgac gtgcgcatcg acgtggaatc cgccggccga ttcgagccct
    16081 ggcagcaggt ggaccatttc ggtgccgccg gcccggacga cgcggtctac ctcgtggacg
    16141 gcgacagcgg cagcgtgcgc ttcggcgacg gcctgcgcgg ccgccgtccg tctgacggcc
    16201 tgcgcatccg ggcctcgtac taccgctttg gcggtggcac ggcaggcaac ctcgcgcccg
    16261 gtgcggtcca gtccgtcgcc gtcggtggcg ggcgcctcaa gccgcggcag gaatggcccc
    16321 tgcgcggcgg cgtggatgcc gagaccgtgg ccgaggccga gcagcgcatc cccgccttcc
    16381 tggcccatcg ggaacgggcc gtcaccgccg aggacttcgc cgtcctggcc cgcgacaatc
    16441 cgctccgccc gatcgcccgc gccgacgccg ccgccggctt cctgcccggc gccagcctca
    16501 ccgcagtgcg gcgcaacgta ccgggcgtca tcagcgtctt cgtcctgccc ccggccgaac
    16561 ccgccttcgc cgccttcccg cggcccaccg ccggcaccct gcgggatgtc ttcgaatacc
    16621 tctctccgcg caccctgctg ggcaccgagc tctacgtgct gagcctccag taccagccgg
    16681 tctccgtcgc ggtctccgtc gaagtggtgg accccgccct ggagcagcag accttccgcg
    16741 ccgtcgagca ggccctcctc gcctacctgt ggcccctgcc gccccacggc gtgcgtggcg
    16801 caggctggcc ccgggggcgc gccgtggaca tcaacgagct gcgcacccag gccagccgtg
    16861 tggacggcat cgaggcggtc aacggcctgc gcctgttcta ccaggatctc gccagcggcc
    16921 tctggcgcga actcaccggg cggctgtccc tgcccctcac cgactaccag cttcccgaac
    16981 tgatggccgt cgccgtccag tccggcgaag ccacaccggc cccgccccgc gccttcgcgc
    17041 ccggcacctc gaccggcgga agcacccgtg ccgtcccggt accggtgatc ccggacgtct
    17101 gctgaaccat ggacagcaac ggcacccgct tcctgctcct cgaccgcgcg gcggatttcc
    17161 gcaacgcggg cggcagctgt gcctgggatg cagacgcccg cgccttcacc ctggtccgtc
    17221 acgacgagcc ccgcctgccc cgtctggcgg cggctgacgc gctccaggcc tgggccgatg
    17281 cagggccgtg gattctggac gactacggcc agatcgcccg gctgtccgcc gaccggcgca
    17341 gcatcgaagc cgccgtcggc tggcccgatg ccccgtggaa ccccctgcgt gcccatcgcg
    17401 accccaccgc ccccctcgcc gccgacctgg aagccctcac gctcgacccg gtcgacgcgc
    17461 ccaccgcgtg cgccttcacc gacctgcacc tgggcggcag cggcctgctc gccgccagct
    17521 ggatggcgga cgacggcacc cgcgccggcg tcacccttgt ccatctgcgc gcccgctggc
    17581 agcgacacct ggagatcgcg tcgccggagg cgccggcctt ccggccggtc cggctctggg
    17641 tcgacgcctc tgaccgggta tgggtcgcca gcgacaccca cctgaccctg accatcggcg
    17701 cccccctcga cccgccctat gttccgcgtc cagaccgctt cgaaccggtc tccgtcgaac
    17761 cgtcgccacc gcgcgtacgg tgggcccagg cgctgccgcc gctccgccgc atcaacgcca
    17821 tcgccgtggt cgccacccgg ctgctgatcc tcgccgaagc gtccgggagc actgacggcg
    17881 caccgcagca gatcctcctc gtccgcgccc tgtccgacga cccgacggct gcgctcacgg
    17941 ccctcccgct ccccaccggc ctccccttcg ccagtgacat cgccgccctc gacgacggcc
    18001 gggcgatcct cctgccgccg cttgaaaccg gcgtcacgcg ggcccagcgg cgcgactgcc
    18061 cggtgatcac cctcgacgat ccacccgccc tcgcggccga gcgctggccg cgccgcagcg
    18121 aagccgccgt gcgcttcgtg cgtcatcggg acaaccaagt gcggcatctg gccaacgacg
    18181 gcgtggtgcg cctgttccgc cttgcccagg cccgcttcct cccccacggc cgcgccctgc
    18241 tgaccccggt tttcgatgcc ggcacgccgg acaccgtctg ggaccgcctc atcctcgacg
    18301 cctgcattcc ggctggctgc tcggtcatgg tcgaagccca ggcagcggac acgcccggcg
    18361 gatttgccga caccttccac cgccagcccc tgcccctggc cttcacgacc cctgacatcc
    18421 ccttcgcgac accgcgcgga acactccacg agatcctcct ccagcgcagc agcggcgccg
    18481 cccgtgaact ccgcgggcgt tatctgcgcc tgcgcctgac gcttggcggc gacggtcgcc
    18541 acagtcccgc cgtgtacgcc ctgagaatct ggtacccgcg cttttcctgg caggcccact
    18601 acctgccgga ccacttccac cagcagcagc ccgccgatcc ggaaccggtt ccggccgggc
    18661 gggcggccaa cggcgccgac ctgcgcgaac gcctcttcgc cagcttcagc ggcctgctca
    18721 cccccattga agaccgcatc gccgccagcg aacaactcct cgacccgctg gccgtgccgg
    18781 ccccgtggct ggccaatctc gccgccatgc tcggcacccg cctgcgtccc cactggccag
    18841 agacccgcca gcgccgctgg cttgccgccc ttggccgtct gcagccccac aagggcagct
    18901 acggtgccct gtgccaggcc ctcgacattc tcacggaagg gggcgtgagc cgtggcgaag
    18961 tggtgccggt cgaactcttc cgcctgcgcc gcacgctggc cacggtactg ggtctgtcca
    19021 tggacgatgc gcgccatccc ctcaccctgg gcaccggcca gtccggcaac agcctcgtcg
    19081 gcgacaccct ggtgctgtcc gaggaccacg ccgtggagat cctcgccctg ttcgcccccg
    19141 aactggcccg ccccggcacc gagcgccagg ccgtcgaaca ctttttcgac cgctacgccc
    19201 gccgcctcac ggtgatcctg cacggccccg cccagtccct gcgcggcgtc atcgaggagg
    19261 ccctgccgga gctcgtcccg gccatcgtgc aatggaacgt actggtcagc gagcacccct
    19321 tcgtgctcgg cctgtcgccc ctgctgcgca tcgacactta tctcgagacc cagccgccgc
    19381 cgaagccggt ccggcttgac accacccgcc tgggccgcgg cgacctcatc tacaacccgg
    19441 tggccctgct gcccgaacag gccggttcgc tgccgccccc cgaatccgaa ggagaccggg
    19501 catgagccag tccgacgaca acgcggccac cgattccagc ctgcccgacg acggcgtctt
    19561 cagcgagccg ctgcgccgcg tcagctttga acccggcatg ctgctcggca tcgaggcgac
    19621 ccgcgccgag caggcctacc accgccgccg ccacacccgc cacgcctact ggctgcacgg
    19681 cgccggtacg gtcgccggcc tgcgcgtggt gatggactcg acgccgccca agaccgccgg
    19741 aaagccgtgg cgggtgcgcc tcatcatcac cccgggcatc ggcgtggacg gcctcggccg
    19801 cgaggtgagc gtccacgaac cctacgccat cgacctgggc gactggctca ccgcccagca
    19861 cgccgacagc accgcctggg gcgccctgga acgggacggt ctcgacaccg ccggcaccac
    19921 cctgtacctg cgcatcacca tgcgctacca ggacgtgccc tccgggctcc agccggtgat
    19981 ggcgacggcg atcaatgccg gcaccgaccc ggtcggtccc agccgcattc aggacacggt
    20041 gctttacgaa ctcaccccgt ggcggcccgc catcggcgac gtccccgaaa ggcccttcac
    20101 cgcccacgac gacctctccg cctacgacga catcgcggac cgcctggccg accacgaacg
    20161 tgccgccatc gccgccgccg atgccgccgg gcgccgccag ctccagcttg gcgcccgcct
    20221 gctgttcgcc ctgtccgacg acaacaccgc cctcgccacc cgcgagtccc tcgccgtgac
    20281 cgcagccgaa ctggccagaa ccctgctcgc aacggtcacc atcgccctcg acccgggcgg
    20341 ccagctcgtc atcgacccgg cgcgcatcgt cgtggacaac ctggcccggc ccttcctctt
    20401 caacgccagc accctggctc gtctgctccg tcagtgaggt catcatggcg aacacgctcc
    20461 agttccagcc ctacgctccc ggcaccaccg ccaccgccgg cggcgcccgc ctgggcagca
    20521 tcgacccgcg cctgtcccgc accaactatt ttgacgggca gctcctcaag gccacggacc
    20581 tcacccgcga ccagatctac ctggacgagc gcctgctgga actcggccag gccttcggcg
    20641 ccggcatcgc cgaggggctg gacctggaca ttgccgacgg ccgctacgtc cgcgtgcagc
    20701 ccggcctcgc catcgccccg tccggacggg tgctcaagct cagcgggcgg gtgctcgaag
    20761 ccgacctgca gaacagcgcc ctcatcgcga gcctcaacaa cggcgcctac cgccagtttc
    20821 cgcggggact ctacgccgtc gtcctgcagc atgccgaagt cgccagtggc gtcgcggagg
    20881 cctaccccac cgacctggaa agccgccgcc agtggaaggt cagcgagtac gccgagggcg
    20941 tcgagctggt cctcgtgccc ctgcccatcg gcctgccccg gggtgacgcc ctgagcctgc
    21001 gcgccaccct ggcccgcgag atcctccacg ccagcggcag cctcatgctg ccctccgacg
    21061 acgccatcgc cctcggcctc gtggccatcg aacaggccgt cgtcgcctgg tacgacaagg
    21121 gcctcgtacg ccgccgcctg cgcaacccgc tggcgcccgc cgcgcaggga gaagacctgg
    21181 ccctgcatta cgtcgagctg ctccacgacg tgctcgacgc ccgcagccac gccggattgg
    21241 gcgacggctt tccggccgcc cagtatttcc gcatgctgcc cgcccatggc ccgctgccca
    21301 aggccgccat cgacccggtg aacggcaccc agatcttctt tcccaaggcc tacgaggtga
    21361 gcattgcgcc cgtgaggcgc agcgacctgc cgcgcctgct ggccgactcc gccttgcttg
    21421 cgcccatcga tctggagcgc gatgcggaca tcgacgtgat ggtcctggtc cccatggacg
    21481 acgccagctt cgccctgcgc gcccgccagc tggaggccga tccggtcacc gggaccctcg
    21541 gcaagacggg catcctcgcc catctggacg tcttcgccct gcgcctgtac gcaccaccgg
    21601 ccctcggcac cgaggaccgc cggacctggg aacagatctg ggctgcggtc gccccatccg
    21661 acgtgcgcta tgtccgccgc ccaccccgca ccgccgagac caatgtctcg gccgtcgtcc
    21721 tcgcccgcgg ctttgcaatc cccgaagaga tcaccctgcc ccagcgggat ctgctcgatt
    21781 cggaacacca gctcgaccag gccctcacgg acatccaggc actccaggtc gaactctccc
    21841 aacagcgccc ccacatccag gacctggaag cccaactggc cacggcgagc ggagacctga
    21901 ccaccgccag gaaccaggtg gacagcctca atgccacgaa caccagcctc accgatgcga
    21961 acgccaggct gacgggcgac cttgccaacc tgaaaaccac caacgacacc cttaccggcg
    22021 acctgcagag cgccacctcc cgcgccgacg aagcggacgc ccgcgccacc gcgctgcagg
    22081 gcaccgtcgc ggaactcaac accacgatca cccagctcca ggcccgtatc gacgaaaacc
    22141 ccgatcagcg cgtcgcccag ctcaccaccg acctggcggc ggcccaggcc gccgccgaca
    22201 cggcccgcca acagctcgcc gatacgacca gccagctcaa cgcgcagcga accgcggccg
    22261 aagccgccac caaccaggcg acggccgcac aggcccgcgc cgacgatctc cagaaccagc
    22321 tcaactccgc caacggacgg gtcgccagca ccgaatcgga tctggcgggt gcccgcgatg
    22381 cactgaacgc cagcaacgcc cggctcagcg cgaccagcgc cgccctggac gcggccaaca
    22441 accgcatcgg tggtctccag aacgacgtca ccaccgcgca aaacgccctc accgtggccc
    22501 gcggtgacgc cgaccagctg cgcggccagg ttgccgggct caatgtcgag ctcagcaacc
    22561 aggcccagat ccaggccggc ctgcgcaacc agatcgccgc ggatcaggcc acccaggccc
    22621 agctcaccgc tcagatcaac cagcaggcgt cgcagatcaa cgccctgaac aatcagatcc
    22681 agctcctgca acgtcaattg gccgacctgc gcgccaatcc caatgcctcc ggcgtcacgc
    22741 tggccgatgt ggcgcgggtc cgtggcgccc aaatggatct gggcctcaag ctcgacgatc
    22801 agttgcgggg tcggccgagc gaagtgctga atgccgccta cgacctgttc ctgctgatcg
    22861 gcgtcgaata cgacgaggtc ctgtggccaa gcctgcagga tctcgcgcgt cagccggatc
    22921 ggctgcccaa gctacgggac gaactggtcc agttgctcac cgtccagcgc atgaccgtcc
    22981 ccaaggccat ggtccaggcg ggccagaacg cgggactgtc cccgaacctc atcaaccagt
    23041 gggccaacca gcgctgaccc cgcccccgcg gacaggagct ttccatgact gcgtcgatcc
    23101 acgtcgaatc tccgcaggcc ggaccggacg atccgcgccg gctcaacctc tttccgggcc
    23161 ttgccctgcc ggagcaggcc tttcgcaagc tccaggacta cacggacgac cgcatggcgc
    23221 ccctcgccgg gcgccttcag cccggcatcc tcgcgggctt cgaggtgagc ctcgaggagg
    23281 cggcccgtgc gccccgcctg cgtttcaggc ccgggcgggg cgtcaccggc gcaggtcagc
    23341 tggttcgcct ctacacaccg ctggtcactg cctggagcga tctcctcacc cttgcggaac
    23401 gtgaccgggg caatgcgcca ctgaaggatg gtctctatct cctcaccctg cgcagcgtcg
    23461 tggaagaggt cgatgcgctc gacacgcgca gcccggccat ccgcaccgaa gcagacccca
    23521 cccgcgaacg ccgtctggag gttgtcaccc tgccgggtct ggtcgagctg tcccgcagcc
    23581 cgcgcctgct ctcgctgccc cagcaggaga ccgtcaaccg cctgagcgtg ggcttcctcg
    23641 acgcctcccc cttcaccctg gcggtcggcg ccgtgccggt ggccatggtc aaggtcgcag
    23701 accgccagct ggcgtggctc gaatcggtcg ggggccgctt tatggcggaa gcggacgcgc
    23761 cccatcgcac cttccttgcc cacaccctgt cggtgctgca ggccggcctg cagcccctgg
    23821 gatcggacgc cagccccgcg ggcttcctgc tgaccgaacg cctcggcctg tcctacctgc
    23881 ccgcggccgg cgcgctgccg cctgccctgc tgcgcaaccc ggccgaccat cctccccagc
    23941 ttgatttcaa tccgtccgat ctgggcatcg acctcatccc ggtgccggcc aacaccgtgg
    24001 agggcatcat tgccgaagaa ctttcccgtg gctacgtgga cctcctccac ccccgccgcg
    24061 accgtctgcg cctgatgctc gccattcccg acctggacta tcggccggat ctcctcgacc
    24121 tgcccctgcg tgacgtgcgg gccgaggcca ccctgtgcga acgcttcgac accgccgagg
    24181 ctgcacgcca ggcctggcgc aaccagtggc agaaggtgtt cggaaacctg gcgcccgtcg
    24241 aactcctcga caccctgctg cagggcaccc agccgcgtct gcccggcgac gtggcggacc
    24301 aggaacccgt tgccgcggag cttgcccggc cggagcggag cgccacccgg gcgcgcgtcg
    24361 aagaagtccg tccgccggtc agcccggacg ccttccgggg cgcccgccgc agcctgctcg
    24421 tcgatgcctt cagcgccggc aagatggccg atgacccggt cttctggctt gacgatcacc
    24481 cgccgcgtta cgaggcggtt cccgcagccc tgcctgaaac cgacaccacc gacggcctgc
    24541 tccgccagcg cgccgacctg cgcgcccaga tcaaggacat gcagacccgc ctcgaaacca
    24601 gctacaagct cctcaacgag ctcaacgact acctggggct gcaacgccag cacttcgacg
    24661 cgctcaccgt gtccttcagc gccctggccg gcggcgtcgc cggcgacggc tcggggctca
    24721 aggtgatgcg ctggaccaac gcggccagct acacgcccgc cagcacaaaa tagggccggg
    24781 agacgcgcat gaacctgctc aacaaatccg gcgtcttcga caacatcgcc gacctcaagc
    24841 tccaggtggg cgtcaaaccc agcgccgcgc tgccccaggt ggaaaaactt tccacccagc
    24901 aggcgttcct gaccctcgag ccccagaccc aggcgatcgc cgccttcgcc gtcgccgccc
    24961 aggccgtcga aagctccgcc gccagcgccg ccgcccgccc ggccgatccc atcgccatcg
    25021 gcaccattgc ccagaccacc cagagcctcg gcaccatccg ccagttcgac gtggtgcaga
    25081 actactactc gacggccctc gagaatctca ccgccctgcg gcgcatcgcc atcgccctgg
    25141 gcgaagacgg caaacccacc ctcgacacca ccaccacgct gctggcccgc gtccgtcgct
    25201 tcctcatcga tcagctgggc agcgaggacg cgctcaacgc gcgcctgggc cagcacggca
    25261 tcgccgcctt cagcaacctg ttcaccacgc tgccccccaa gatcggcgcc accttcactg
    25321 acgccgacag caagagcgcc acccataacg cccactcggc cctgttccac cacggcgccg
    25381 tgatcaccga cgacatccgc ggcatcgaag ccctgcgcgc cgtcctggtg gccctgcgcc
    25441 aggaccacca gaaccagctc gcggccctcc aggaccagct cgccaccgtc gaaacccagc
    25501 tcccggaggc cgccgcccgc ctcgacaccc tcaaccacac ccgcctcgaa gccctcgacg
    25561 actacgccgc tgcccagcac accctggccg agcactggga cgccgtcgcg caggcttacg
    25621 ccgaacgccg ccgcgtcatc gacagccacc ggggactgtt ctacgtcaaa cagcgcgaga
    25681 cgccggtcac cgtgaccctg cccgaccccc aggacctgcg cttcacctcg ccggacgata
    25741 tcgtccccgg cgtgcccgac cgggagattc cgctcgccga cgacctcgcg cccttcatgg
    25801 aggtcgtcct cgacatcccc gtcgcggact gggccgtgct gtcgggcctg gcccacctgt
    25861 tgccgagccg cccgcgtctg gaactgatgg tcgccacgcg cctgcagaag gtcgccctgc
    25921 gccagaagat cggcctgccc gtcgccaccg cgcgtctgca ggggctcgcc ttccagaacc
    25981 gcggactcct cgacgacatc gccctgcgcc cctttgccgc cagcgccatg ctcgacctgc
    26041 aacgccaggg ccacgccatc ctggccctcg atgacctcct tgccagcccg gttcctctgc
    26101 tgcgcgaacc cgcccgcgtg ctgcaccgcc gcctcgatgc cgccgccggc gggctgctgg
    26161 acctgctgcg ccccattgcc ccgtccctgc gcctggcctg ggccgccgcc gccgaggccg
    26221 acaccctgcc cgtggagcgc ccggaacgct ggcccggcat cgcccaggcc gaagccgccg
    26281 acctcaacgg cctgcgtaca ttcggtgagc tcgttgcctg gtggttccgc cagctcgaca
    26341 gcgacgccag cgccgcgtcc cgcacggcca tgcgcaactt cgtccgtgcc tgcgtcatgc
    26401 tcgccaccgg cgacaacccg gacgagatcc tccagggtca cgtcagcagc ccgccacgcc
    26461 gcttccgcat cggcgaggtc atgcgcctcg aactcaaccg cgagccccgc ccgggcgccc
    26521 tgctgcagct cctcgacgat gcccagcggc tggtcggcac gctgcgggtg gacgaccacg
    26581 acaacggcca gacggtggcc tccatcgtcc gcgtgctgga cgacagcctc ggcgtgaacc
    26641 ggcccctcac cacggcattc cgtgtgaccg gcagccgcaa tccctagccc ggacaccgcc
    26701 ccatggaccg cgcctccgcc accgtcacca tcgcccgcct gaacctgcgc ggcgcccggg
    26761 acgacgtggc gcgggcacgg acgcccctgc tggccgcgct ggatcgggca ccgtggcccg
    26821 acccggggcc ggacaccctc ctcttcgtgc gccacctgcg cctgcgcggc tccgccaccg
    26881 acatcgcccg tcgtgctgcc gacctcgtcc gcgaactcac gccccgtgcc gaggacggct
    26941 ggagccgggc cgccgaccag gccgacgcgg tgcgcttccg atcccgcgcg gacatgtccg
    27001 cctgtctgct tgccgacctg ctggacggcc gcgccgccga tcgctggttc tgggcacgcc
    27061 gggccgaact gttcgaacgg cccgtcccgg acgccatcgt ccggatcctc gaagaggcgc
    27121 ccttcgaact gcccgaactg ctcgatgccg ggtccgcccg cgcccgtccc ctcgcggcca
    27181 tcgaactcct gccgcgcctg atggcccgcc tggacgcgcc ggccaccgag cgcctgctcg
    27241 gcgccctgcg cgcggccacc gggtggtcgc tgcgttttgc ggcccccgag gccaccgcca
    27301 tggcgcctgc gcccggcctc ccgaccgccc tgctcgccac cgccacacgg caggcccacg
    27361 cccttcgcct cgcgcccgcg gcccccgcgg cgcggctcgt ggccgtgctg ctcgcctggc
    27421 gccacgttcc cgcgcacctg ggcacaccgg acgccgacgt cctgctgacc cgcctcacgc
    27481 acctcgtcac ccaggatggc gcccggagcg ccccgcagca gccgatgccc atcgcccggg
    27541 acgccacagt gcctgcgcct gccgacacgc catcaccgca ccctgcgccg ctgccgacgg
    27601 ccgctcccgt cgaggcgcgc accaccggcg cgcatcccgg gcggaccacc acgacatccg
    27661 atgcgtcgcg cgagcccgca tccgacgccc cggccgccga atgcgccgca gccgccctcc
    27721 attcaccgcg cgccgccgcc cctgaagacg gctttgtcac ctcgtgcggc ggtctgctct
    27781 tcctgctgaa cgtccttgcc ctcacgccgg tgcagagcct gctcggagac cggaacctcc
    27841 cgggctgcgg ctggcgctgg ctggcgcgtc tggccgccac cctcggactc gagccggacc
    27901 caccgctggc cgcgttcttg gcgaccgaag ccgccctcgc cagtccggat gccctcgccg
    27961 tcctcccgcc gctggccgaa gaggcgcgcc tggaggcctt cgccgcagcc cgctatggcg
    28021 cggatctgtg gcgcgctgac gccttccgcc tgcccgcccg gattgtcgcc acccccagcc
    28081 acgtggacgc ccacttccgc cttgccgacg cgcgcctcga tctgcggcgc gccgggctcg
    28141 acctcaaccc cggctggctg ccgtggctgg gccgggtggt caccttccac ttcgacgacg
    28201 gcccgcgtgc actgcccgcc ccaatgggca agcccgaaga cttcacgatg gggcgcgacc
    28261 actcgcccgc cctttcgccc gccggccctc ccgacaccgg acccgctgcg cccgcagccg
    28321 ccaaaaagcc cggggaacga ccatgaacgc cccgctcgcg tccgcccgcg cgctggatgg
    28381 agcggtcctc aaccgcgcct tcgtccgggc cggtctggca cgtttcggac tcacccgact
    28441 cgctgcgccc tccggtctct ttgccgagct cgcggccgat gccggcttgc attgcctgct
    28501 ggacagttcc ccggacgact gggccgccgc ctgcgcggcc ctggccgccc gagccccgag
    28561 ccttggcggt gcctggggcg ccctcctgac cgactaccgc cttcagctgc ctgaatggtt
    28621 cgccctggcc ctggccgccg aagtggaggc ggatcacgcc gtcggccggg ccctcgccga
    28681 gctgcaggcc cccgacgcgg acgggcgacc cgccctgcac ctcctcgacg ccctcgccgc
    28741 gacgctcttc gacgcggccc ttccgcccct cgcgtggccc caccacgccc tcgtccagcg
    28801 cggcctggcg agcctgtccg gcgacctccc cctggtccgt accagcctgc gtgtagaccc
    28861 gcgcctggcc gccctgctgg ccggagaccg acgcccgtgg gcagacttca cgccgctgcc
    28921 gccggacacc ctcacgcacg tgctacccgc caccctgctc accgaactgg acgcagccgc
    28981 gcacctggtg cagcagggcg ctgcccgcct cgtcgtcctg cggggcagcc acggcgccgg
    29041 tcgggcggct gcggcacgcc ttggcgcggc cctggggctg cttccagtga ccattccacc
    29101 cgacccctgg cgcagcaccc cgatcttcgc cgcggcctgc cgctacgggg gctggctgcc
    29161 cgtcttcctc acagacctgg ggcccggcga caccttcagc ctgcccacgg gctcgcttga
    29221 gcagacgccc caggtcctcg tcaccggtca ggacggcagc gtggatacgc ccgacgccat
    29281 cgaactggac atcccgcccc tcgaacggca cgagcggcac cgggcctggc aggccgtcct
    29341 gggccccgac gccccgcccg tcctcgccga tcacgcccgg ctggacggcc ccgccatcca
    29401 caccctggcc caggccgtgg gccgcgacgc cgcgcgccgc cacgagatgc cggcactcgc
    29461 ccacctgcgc cgcgcccgca gcacccacgg cgccgaacgc ctgcgccagc tggcccaacc
    29521 ggtgcaccgc gacgtgggcc ccgatggact tgtcctgccc gccgagacgg cccgccagtt
    29581 cgaacatctt gtgcgccgct gccgcatgcg cgaacgcctg tgggacggcc tcggccccag
    29641 cctcgccagc cccgcaccgg gggtgcgcgc cctcttcgcc ggggagagcg gtgccggcaa
    29701 gaccctcgcc gccagccgcc tcgccaccga gctgggcgca ccgctctatc gggtggatct
    29761 ggccgccatc ctcaacaaat acgtgggcga gacggagaag aacctggggc gcctgctgga
    29821 cgaggccgcc accctggacg cgctgctcct gatggatgag gccgacgccc tctttggccg
    29881 acgcagcgag ggcaaggatg gcggcgagcg ttacgccaac atgatgacca acttcctgct
    29941 cacccgcatc gaaagccacc cgggcatcgt catcctcacc accaacgccc gcaaccgcct
    30001 cgacgccgcc ttcacccggc gcttcgacac catcatcgaa tttccgctgc cgggcgtggc
    30061 cgagcgcctg cgcctgtggc acgcccatct gggcgcccgc agccccggcg aagccgcctg
    30121 cggcctgctc gccacctaca gtgacctgcc cggcggccat atccgcaatg ccgtgctgta
    30181 cgccgtcgcc ctcagccccc gcccgcccgg cacgccggtc gagatcaccc tgctggtggc
    30241 cgggctcgcc gaggaatacc gcaagctggg ccgcaccccg ccgccccagc tggcccagat
    30301 cggggaatga accatggctt ccctcacacc cgtccgccgc gccgccaagc gccccgacct
    30361 tgcccgcgca ccggcaccaa aggacgacaa gcgtcctctg cagcgggccg cgacgccggc
    30421 caagtccccg tcgcccccgg cttatctgca ggccaagctc gccatcagcc acccgagcga
    30481 ccactccgag caggaggccg accgggtggc cgcccaggtg gcccgcagtc cccgtccagg
    30541 cgaacctgaa acgccgcagg cgacgcccgc ggcccgcctg cagcgcgcca gcctgcaacc
    30601 gggtgtcgaa gcggacgacg tcgccccggt gcccgccgcg cgccacctcc accgcagccc
    30661 ggcgccggcg cagacgccgc ccaccgtcca ccgcaaggcc gcccccgacg ccctggccgg
    30721 acagcccgcg tctgccgaca cggaggcgcg catcgaagca cgtcggggcg gcggcgcccc
    30781 tctgccggac gccgcccgcc gggacatgga agcccgtttc aaccaggact ttgcggccgt
    30841 ccgcatccat accgacgcgg acgccgccga cctgtgcggc gagacccgcg cccgcgcctt
    30901 caccgtcggc agcgacatct tcttcgcgcc cggagaattt gcccccgaac gtggccccgg
    30961 gcgggagctg ctcgcccacg aactgaccca cgtggt
//
LOCUS       DAOQMM010000033        30487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_406_length_30487_cov_54.476899, whole genome shotgun sequence.
ACCESSION   DAOQMM010000033 DAOQMM010000000
VERSION     DAOQMM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 30487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30487
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_406_length_30487_cov_54.476899"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1433)
                     /gene="clpX"
                     /locus_tag="PLH85_07655"
     CDS             complement(165..1433)
                     /gene="clpX"
                     /locus_tag="PLH85_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNB64475.1"
                     /translation="MADKKTSGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCN
                     DIIRDEITADTGAKGVKGELPSPKEICAILDQYVIGQGSAKRILSVAVYNHYKRLRHL
                     SKSHDEVELAKSNILLVGPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVE
                     NIIQKLLQKCDYEVDKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEG
                     TIASVPPQGGRKHPNQDFVQVDTTNILFICGGAFDGLEKVIRNRTEKVGIGFGAEVKS
                     RATADVSKTLREVEPEDLIKFGLIPELIGRLPVVATLQELDEEALIQILIEPKNALIK
                     QYQKLFSMEGVELEVRPVALQAIARKALKRKTGARGLRSILESTLLDTMYELPSMENV
                     AKVVIDEGTIDGAAKPILIYADQPKVSGSN"
     gene            complement(1478..2122)
                     /gene="clpP"
                     /locus_tag="PLH85_07660"
     CDS             complement(1478..2122)
                     /gene="clpP"
                     /locus_tag="PLH85_07660"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNB64476.1"
                     /translation="MNFMTPPDSRSNWDPVGLGLIPMVVEQSGRGERSYDIYSRLLKE
                     RVVFLVGPVTDVTANLIVAQLLFLESENPDKDIFFYINSPGGSVTAGMAIYDTMQFIK
                     PDVSTLCVGQAASMGAFLLAAGAQGKRFSLPNSRVMIHQPLGGFQGQASDIEIHAREI
                     LSLRAKLNDMLAKHTGQPVETIEKDTDRDNFMSAEEAMRYGLVDKVLTSRADVA"
     gene            complement(2144..3448)
                     /gene="tig"
                     /locus_tag="PLH85_07665"
     CDS             complement(2144..3448)
                     /gene="tig"
                     /locus_tag="PLH85_07665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNB64477.1"
                     /translation="MQTNQETVSALERRIDMTVPLADIEKDVEARLKQMARTVKMPGF
                     RPGKVPFRMVAQSYGSQARSEAVGAAVERVLAEQIRAQNLRIAGYPRIEPKDAASAEA
                     LEFSAVFEVYPEVVVGDLSAREVERPILSVTEAEVDKTLEVLRKQRTTFVPAERAAQD
                     GDRVTVDFTGRKDGVEFDGGKATDFPFVIGAGQMLKDFETAVVGLSAGESKTFDMTFP
                     DDYHASHLAGQAVQFEVTVKQVAEPKLPEVDGEFAKSLGIADGDTSKMREEVKANLER
                     EVKRRIQARIKEQVMNVLLDAHPIEVPKALVEQESQQLADNARKDFESRGMKTKDLPI
                     STDWFTDQAVRRVKLGLIMSELVRSKELFAKPEQIRAIIEDFAASYEDPSEVVSWYYA
                     QPERLAQAEALVVEDNVVAWVVGNAKATDKETSFEELMGNAA"
     gene            3844..5766
                     /locus_tag="PLH85_07670"
     CDS             3844..5766
                     /locus_tag="PLH85_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNB64478.1"
                     /translation="MSIFTSFQSRFESAREEEMSVQEYLDLCKADPLAYATSAERMLQ
                     AIGEPELVDTRLDPRLSRIFSNKMIKIYPAFRDFYGMEEAIENIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKSLVERVPFYAIKGSPVHESPLGLFSAGEDGEILESD
                     YGIPRRYLNTIMSPWAVKRLHEFNGDITKFRVVKLRPSVLRQVAVAKTEPGDENNQDI
                     SSLVGKIDIRKLEQFSQDDPDAYSYSGGLCLANRGMLEFVEMFKAPIKVLHPLLTATQ
                     EGNYKGTEGFGAIPFDGIVMAHSNESEWTAFKNNRNNEAFLDRIYTVKVPYCLRVSDE
                     IRIYDKLLTNSSLSEAPCAPDTLRMMAQFAVLSRVKEPENSSIYSKMRVYDGENLKDT
                     DPKAKSYQEYRDYAGVDEGMTGLSTRFAFKVLSKVFNFDHREVAANPVHLMYVLEQQI
                     AQEQYPPENEAKYLGFIKEFLAPRYAEFIGKEIQTAYLESYSEYGQNIFDRYCTYADF
                     WIQDQEYRDQNTGEILDRNALNDELEKIEKPAGISNPKDFRNEVVNFVLRARAKNAGK
                     NPSWTSYEKLRAVIEKKMFSNTEDLLPVISFNAKASGDEQKKHQNFVNRMIEKGYTEK
                     QVRLLCEWYLRVRKSS"
     gene            5830..>6692
                     /locus_tag="PLH85_07675"
     CDS             5830..>6692
                     /locus_tag="PLH85_07675"
                     /inference="COORDINATES: protein motif:HMM:NF003707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNB64479.1"
                     /translation="MVRIVDRRLDSKNKSAVNRQRFMRRFKNQIRRAVSDAIAGRSIK
                     DLDNGESVSIPAKDLAEPQFHHGRGGIWETIYTGNDQFSSGDQINRPQGGGGGGTGKG
                     KASNEGEGEDDFVFNLSREEFLDVFFDDLALPNLVKTQLARIHEYKIQRAGFTNDGTP
                     ANINVVRSMRGALGRRLALASPYAGKLRELQKQLDELLETRPDDDPDVEKLRHDIAVL
                     RAKIEAIPFIDTFDLRYNNRVRIPKPTTQAVMFCIMDVSGSMDEEKKSTAKRFFMLLY
                     LFLTRTYEHIEV"
     assembly_gap    6693..6792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6793..7253
                     /locus_tag="PLH85_07680"
     CDS             <6793..7253
                     /locus_tag="PLH85_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HNB64480.1"
                     /translation="FFMLLYLFLTRTYEHIEVVFIRHHTVAKEVNEEDFFHSRESGGT
                     VVSSALELMRDILHERYLNGAWNVYGAQASDGDNWENDSPHCRELLAETILPFVQYFA
                     YIEITPGEPQNLWREYEKLTGAKSNFAMQHIEGLPDIYPVFRELFKKKLA"
     gene            7250..8779
                     /locus_tag="PLH85_07685"
     CDS             7250..8779
                     /locus_tag="PLH85_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNB64481.1"
                     /translation="MSARRPKRKKALPAPGEWTFDLIETYHAEIDRVARDFGLDTYPI
                     QLEIITSEQMMDAYSSVGMPVNYHHWSFGKSFLHTEKSYRRGQMGLAYEIVINSNPCI
                     AYLMEENTMTMQALVIAHAAYGHNSFFKGNYLFRQWTNADAIIDYLLFARNFLGECEQ
                     RHGEEAVELLLDSCHALMNVGVDRYKRPAKLSLEKERTRQRERAEYLQSQVNDLWRTL
                     PIQQIKAQAVEERRFPPEPQENLLYFIEKNAPLLEPWQREVVRIVRKVGQYFFPQRQT
                     QVMNEGWATYWHYTLLNTLYDEGLLSDSFMLEFLHSHTNVVYQPRYSEPWYRGLNPYA
                     LGFAMWQDIRRICEHPTDEDRAWFPDMAGSDWRETFDFAMRNFKDESFIAQFLSPKVM
                     RDFRLFAILDNDKDPKLKVSAIHDESGYRRVREILSDQYNLGSREPNIQVWNVDLRGD
                     RSLTLRHQAYMRRPMGGDAEEVVKHIGRLWGFPVRLEGVQDDGTVQKLHEWSPKHAQD
                     A"
     gene            8862..9854
                     /locus_tag="PLH85_07690"
     CDS             8862..9854
                     /locus_tag="PLH85_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNB64482.1"
                     /translation="MSADDFHRILGVKPGASAAEIKRAYRRQAMRWHPDRNADPDAHD
                     QFKRIREAFERLSVGASQVDNDGDADTPPPAAPRGEDRRVEVTLDLTEAALGTTVDVT
                     VEGRTSCTACGGSGQRSYGRTTMCGACHGSGRVRSKNGLDNCTQCSGKGYFTDRRCPD
                     CDGRGWHPSERRIEVTVPPALLPGDELRVPGQGGPAPEAGTPGDLYITVRARPHPLFR
                     LDGRNVHLTVPVSVFRLLAGGEILVPTLSGLETVQLPEGAGQPELRVTGHGFPARGKR
                     PAGDLHLHLKTELPAYLSQEQKAMLEMAEQVLLHHLDTQSPALALWKQVLEQHR"
     gene            9908..11176
                     /locus_tag="PLH85_07695"
     CDS             9908..11176
                     /locus_tag="PLH85_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNB64483.1"
                     /translation="MPPKTPELLAPAGSLAMLDTALAFGATALYAGQPRYSLRVRNND
                     FGDLPILAEGIRRTHAIGARFYLVANIYPHGAKVKTFLKDIAPVIALAPDALIMSDPG
                     LILLVREHFPHMPIHLSVQANTVNAAAVRFWRQAGISRVILSRELSLDEIASIRQDCP
                     DTELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNACRWEFKTHGARTCSAGDVH
                     LLEEPQKRAGEYMPIEEDEHGTYILNSRDLRAIEHVERLVEMGIDSFKIEGRTKSPYY
                     VARTVQAYRQAIDDAVAGRRLDPALLGQLEGLANRGYTDGFYRRHTPHATQNYLRGHS
                     ESGRSLYVGDVVAFDAARGLAEIASRNAFSVGDRIELIQPAGTREIRIDRLEDADGAP
                     LERVPGSDHRAWIPLPPDCVGAFVARFIHP"
     gene            11269..11487
                     /gene="rpmE"
                     /locus_tag="PLH85_07700"
     CDS             11269..11487
                     /gene="rpmE"
                     /locus_tag="PLH85_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNB64484.1"
                     /translation="MKADTHPKYAEVEVTCSCGNVFTTRSTLGKPQLHVEVCAACHPF
                     YTGKQKIVDTAGRVERFRQKYGNVQRLG"
     gene            11554..13179
                     /locus_tag="PLH85_07705"
     CDS             11554..13179
                     /locus_tag="PLH85_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64485.1"
                     /translation="MPVPPKSALLPTPLTGWPLAVLIGVYLLVGMGHVPWRGDDLTYL
                     GPIHAILTDGSWLIPSIAGEPFYEYPPLYYWLGAVLARAFGWLMPVHDAARLASSLCA
                     AATLYWTGLAARRLYGDNTGAPAILLGLGSLGLVVHAHETQPLLAQLAGMALCYAGLA
                     DMVRRPWAGARQAGIGAGLTFLAGGLPGMFVTLPLFFAMPLLCDNCRNGAVPRALAGG
                     LVVALLLGGSWPALLGWLHPEALASWWNDELAASLPHGNHLQALGKMIPLLGWFAWPL
                     WPIAGWALWKNRRALNTPPVALPIIASLLAMVAVVTTGPLRPASTLPILPPLCLLAAL
                     GVGTLRRGAANALDWFAIMAFAFFAILVWIGWSALYFGWPPGLGRQLARLAPDLVRSP
                     STIAAVTGMFLSLALFALPVLTTRSTLRGATNWAIGMTLLWCLAVALWQPWFEHTKNY
                     APVAQALARELELHPAPCITRKGLNDTQRAALDYFNGIKTIHHSADQDCPAFLVYSAD
                     GLPYRPPGDWRTSWEKRLGGGRKTELFRLYRHD"
     gene            13172..14806
                     /gene="pgi"
                     /locus_tag="PLH85_07710"
     CDS             13172..14806
                     /gene="pgi"
                     /locus_tag="PLH85_07710"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNB64486.1"
                     /translation="MTDLTPFDLDASDAGTSLSRLASAPELTHLRALFSKDPARFQAF
                     SCRYGEWLLDYSRQRMDAAIRSQLLALAEESRLRQGIDAMLSGAIVNVTEQRAVLHCA
                     ARLSPHTPLNARNQIEETLARVMALVDRIHAGSLTGSRGMPIRHVVHLGIGGSDLGPR
                     VLSEAFSTAYPRRLKVSFAANVDASEIRSILIDATPEETLFVVASKSFTTAETLSNAR
                     IARQWLTDQLGPDADVRAHFAAISNNVHAASSFGVPEERIFPLPEWVGGRFSVWSAIG
                     IPIMLEFGTPVFLEFLAGGRDMDAHFATAPFDSNLPVLAALIGLWNASYLRINCHAVL
                     PYCHALRSLPAYLQQLEMESNGKHVRRDGSALSYPTAPILFGGAGTVGQHAYHQLFYQ
                     GTETVALDFIVPIPDTPTPATRSLVGNALAQAAALMHGRTEDEARAELTQRGLAQDEV
                     ERLAPHLVCPGNQPSSTLLMPALTPFTVGQLIALYEHKVFAQGWMWGLNSFDQYGVEL
                     GKQLARRLESGDMSDADSSTQGLMADIARRWEPAKE"
     gene            complement(14872..15153)
                     /gene="ftsB"
                     /locus_tag="PLH85_07715"
     CDS             complement(14872..15153)
                     /gene="ftsB"
                     /locus_tag="PLH85_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNB64487.1"
                     /translation="MRWPLLILVLLVAALQYPLWIGKGGWLRVWEVEQQLAEQKEGNR
                     KLEQRNTGLEAEVRDLKSGYEAVEERARFELGLIKPDEVYVQVPQKTPQ"
     gene            complement(15201..16490)
                     /gene="eno"
                     /locus_tag="PLH85_07720"
     CDS             complement(15201..16490)
                     /gene="eno"
                     /locus_tag="PLH85_07720"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNB64488.1"
                     /translation="MSAIVDVIAREVLDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDAARYLGKGVLQAVENVNTEISEALVGLDAEEQAFLDRTLIELDGTEN
                     KSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGGMSMPVPMMNVINGGAHANNSL
                     DIQECMIMPVGAKSFREALRCGAEIFHHLKKLTDKKGYPTTVGDEGGFAPNVSGTDEA
                     LNMILEATANAGYEPGRDVVLALDCAASEFFKDGKYELHGEGLSLSSEGFTDYLATLA
                     DKFPIISIEDGMAEGDWAGWKILTERLGKKVQIVGDDLFVTNTRILKEGIEKGIANSI
                     LIKINQIGTLTETFAAVEMAKRASYTAVISHRSGETEDSTIADIAVGLNAMQIKTGSL
                     SRSDRISKYNQLLRIEEDLGDTAFYPGLSAFYNLRTR"
     gene            complement(16585..17424)
                     /gene="kdsA"
                     /locus_tag="PLH85_07725"
     CDS             complement(16585..17424)
                     /gene="kdsA"
                     /locus_tag="PLH85_07725"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNB64489.1"
                     /translation="MKLCGFDVGLDKPFFLIAGPCVIESREMALDTASALKEICAELA
                     IPFIYKSSYDKANRSSGKSFRGMGMEKGLEILADVRATVGVPVLTDVHTEDEVPAVAA
                     VVDVLQTPAFLCRQTDFIHAVAASGKPVNIKKGQFLAPGDMKNVVDKAREANGGADTI
                     MVCERGASFGYNNLVSDMRSLAIMRDTGCPVVFDATHSVQLPGGQGTSSGGQREFVPV
                     LARAAVAVGVAGLFMETHPDPAKALSDGPNAWPLPRLKALLKTLKDIDALVKGQGFIE
                     TQA"
     gene            complement(17436..19079)
                     /locus_tag="PLH85_07730"
     CDS             complement(17436..19079)
                     /locus_tag="PLH85_07730"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNB64490.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMGKRNNFTTGQIYEAVIKKE
                     RRGEYLGKTVQVIPHITDEIKLYIKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
                     IEEGRQGTCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPAD
                     ERRKIALFCNVMPEAVIECLDADSIYSIPGMLHDQMLDEIVCHKLGILARAADLSVWE
                     RLIDALNNPQHHLDIAFVGKYVDLTESYKSLIEALNHAGMHTRSKVSIHYIDSEDIEK
                     SGCDVLKRMDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDV
                     AGMNGAHSTEFDKHTEFPVIGLITEWLDASGKLEKRGEDSDLGGTMRLGGQVCRLTEG
                     SLAREVYGRPEITERHRHRYEVNNSLLSRLEAQGLRVAGRAPGTDLCEMIELPTEGPN
                     AHPWFVGCQFHPEFTSNPRQGHPLFTAFVKAALAHQQAG"
     gene            complement(19185..19727)
                     /locus_tag="PLH85_07735"
     CDS             complement(19185..19727)
                     /locus_tag="PLH85_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNB64491.1"
                     /translation="MLMDRRCASLLVVDVQERLLPAIDDGAAVLDHCVWLVRVARELG
                     VPVVLSEQYPQGLGPTATALQEAAGDAAVVYKTAFSCVADGSLSGTPVDARRQVVVTG
                     TEAHVCVLQTALGLRWQGKEVFVVAEAVGSRKPADKELALARMRAHGIEVVSREMVAF
                     EWMERCTAPEFRTVSRNFIR"
     gene            complement(19738..21714)
                     /locus_tag="PLH85_07740"
     CDS             complement(19738..21714)
                     /locus_tag="PLH85_07740"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNB64492.1"
                     /translation="MSYPIAAAPLWTPSPERVAAALITGFAQRASARWQRPLDGYAAL
                     HAWSVAHPEEFWTSVWEDGDVCGVRGDTVLADGERMPGARWFPDARLNYAENLLRSRD
                     DADALVFWGEDKVKNRLSHGDLYRQVAHFAAALQEQGVGAGDRVAAWMPNLPETIVIM
                     LAAASIGAVFSSASPDFGVQGVLDRFGQIEPKVLVAVDGYYYNGKVVDCRDRLAAIAD
                     GLPSLRRVVVVPYVHASHDLSHIHHARLLPEFVRPFLAETEIPFVRLPFAHPLFILYS
                     SGTTGVPKCIVHSAGGTLLQHIKELRLHGDVRPGDRLFYFTTCGWMMWNWLVSGLASG
                     ATLLLYDGSPMVGQGSILFDFAEAERMTHFGTSAKFIDGIAKLGVAPRETHDLTSLRA
                     MFSTGSPLVPEGFEYVYRAVKADLCLSSISGGTDIISCFVLGNPTLPVYPGEIQCRGL
                     GMAVDVFDETGTPIQGEKGELVCTAPFPSMPVGFWNDPDGSRYRAAYFERFEHVWCHG
                     DYCEITAHAGLIIYGRSDATLNPGGVRIGTAEIYRQVEKCDAVLESLVIGQQWPAGSG
                     DVRVVLFVKLRDGRVLDDALCEQIRQTIRDNATPRHVPARIVQVDDIPRTRSGKIVEL
                     AVRAVVHGEPVKNLEALANPAALDNFRSRPELVN"
     gene            complement(21763..22185)
                     /locus_tag="PLH85_07745"
     CDS             complement(21763..22185)
                     /locus_tag="PLH85_07745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB64493.1"
                     /translation="MNTPASAPDRKLLLTTRIPVRWGDMDFYGHVNNTVYFRYYEQAR
                     VEWLEAFGFAVGPTAETAPVIINASCTFLIPVNYPATVIVRLYAGAPGRSSVMTWYEM
                     GVEGDERVFATGDCKVVWMDTRTGKSAPLPDALRAAVA"
     gene            22405..24045
                     /locus_tag="PLH85_07750"
     CDS             22405..24045
                     /locus_tag="PLH85_07750"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNB64494.1"
                     /translation="MERDSMDFDVLIVGGGPAGLSAAIRIKQIAAEKGQDVSVCLIEK
                     GAEVGAHILSGAVVDPRALNELFPDWQALGAPLTTPVSEDRVIFLSETGGLKVPNAFL
                     PDSFHNAGNYIVRLGNLAKWLGEQAEGLGVEIYPGFAGAQLLTGDNGAVLGVITGDMG
                     LLKDGTEGPNFQPGMELRAKYTLFAEGCRGHLGKELEARFNLREGVDPQTYGIGLKEL
                     WEVPAPQHVQGLVVHTAGWPMEASTYGGGFVYHLEDNLISVGYVVGLNYSNPFLSPFE
                     EFQRFKTHPEIRKFLEGGKRLAYGARAIAAGGLQSQPRLVFPGGALIGDDAGFLNAAR
                     IKGTHAAMKSGMLAAEAVFEAVTAGRAQDSLDAYPAAFRESWLYSELHKTRNFKPYMK
                     KGLWMGSLLFGIDQKVFGGKLPWTLHNHADHTALRPAAECTPIAYPKPDGKLTFDRLS
                     SVFLSNTNHEEDEPCHLRLADASIPISVNLARYDAPEQRYCPAGVYEIVRSDDGAARL
                     QINAQNCVHCKTCDIKDPTQNINWVTPQGGEGPIYQGM"
     gene            24156..25295
                     /locus_tag="PLH85_07755"
     CDS             24156..25295
                     /locus_tag="PLH85_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNB64495.1"
                     /translation="MKTAQIVLNGSSVSSSLATLTAIQPHLVFVFADPSWCVGEDRLS
                     PLLQALTPAQLVGCSTAGEVAGHGVQENSCVLTAVHFDDPAFTVTRTHVDDMRDSRAA
                     GERLAVALAPHSPHSVLVFGPGVRLNGSSLVAGMAAGLPGATTLAGGLAGDAGHFRTT
                     WTIGPDGIRERDVVAVGFHSPRLKVCHGSFGGWTPFGPVRRVTRAHENILYELDGERA
                     LDVYRRYLGDYAKDLPASGLLFPFAMLNGDRDETGIIRTILGIDAEAGSLLLAGEIDP
                     HGYLRLMHASTDRLVDGAAEAAARIGKAPQRSDGLALLVSCVGRKLVMGDRVDEEVEA
                     VARVLGPTSCLAGFYSYGEIAPHGADADCTLHNQTMTITRLHEEA"
     gene            25295..28420
                     /locus_tag="PLH85_07760"
     CDS             25295..28420
                     /locus_tag="PLH85_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB64496.1"
                     /translation="MPDVFHPLLLRQLRRTCSLGDAAELETALAELRKASLDGRLDGS
                     AAALAHGLERLFERVGGGYRQLERDLDLRTRSLEHSSAELNALNARLQDDLESRSRAL
                     AALRTTLIELQDAPAAPSADASSEGLEATSHLLARLVESRQQKARELDYQKFALDQHA
                     IVVVMNAHGIIEYANDRFCRVSGYQSDELVGRHRHMLTSRDHPRSFFIDLWRAVNGGD
                     VWHGQICNRQRDGRPYWLDTTIVPFRDGGGAISRHIAICTDISAMKQLEQALREQLAL
                     IETLFETIPIPIYIKDAGGHYQRFNKAFEEFFGIRREDWLGRTALEVLPESLARRHVA
                     LDEEVLGCGGVQEFEFEWPFPGGTRVGDYRKVALRDPHGAVTGLIGVIHDITPRKQAE
                     EAMRQARDAAENANRAKSDFLANMSHEIRTPMNGILGMTELTLDTDLNAEQREYLQLV
                     QTSAESLMGIINDILDFSKIEAGKVLVEHIDFDLHRTVTEALKTLAVRAYEKNLELLC
                     DIDPAVPQRISGDPGRLRQVLLNLTGNAIKFTPGGEVAVTVRYAADAGEAGPRIHLAV
                     RDTGIGIAPDKHTAIFEPFSQEDGSTTRRFGGTGLGLTICRRLVEAMGGRLWLDSTPG
                     TGSTFHFTLPAANPAAAPREAPPAVLAGRRVLIVDDHPTNRLVLERSLRRIGMQACST
                     GASDHVVAILNASQCEGTPKEVVLIDSCMPGMDGFTLAQAIVAEGFDPCPALVLLSSA
                     GAKGDAARCREIGIEAYLTRPVSQDEISQTLQRVMAARPRPAPRSLVTRHVLGEDGPR
                     LNLLVLEDQAVSRRLMIALLTRLGHQVELADTLAGIAPAARRFGGPVDVVLASLQVPE
                     LPPSRVIAGLRAAGIDAPVIGVTADPDEDVRNACVAAGIADVLAKPLQTGALRAALDV
                     LAATRCDVRDAARHATAAPRFDYARALLDADREIVDMIGEQLLAQLPGEIAQLRSSLS
                     GAPPLTIQARIRTLKGLLDTVRAEPAIRLLDELALRLSQSDTPYADDLLATLDAEIAE
                     FTRALRARRPG"
     gene            complement(28500..>30487)
                     /gene="purL"
                     /locus_tag="PLH85_07765"
                     /gene_synonym="purI"
     CDS             complement(28500..>30487)
                     /gene="purL"
                     /locus_tag="PLH85_07765"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNB64497.1"
                     /translation="ITIGDRSVGGMTARDQMVGPWQVPVADVAVTTMSFLGYRGEAFA
                     MGERTPVATLDAPASGRMAIGEAITNLAAADIASLGDVKLSANWMAAAGHRGEDVKLF
                     RTVEAVSRFCQSAGLSIPVGKDSLSMRTAWHDDGEDKQVVSPLSLIVTAFAPVQDVRR
                     TLTPQLQLPEGVETELLLIDLGNHRNRLGGSVLAQAYNSVAEHAPDVDPAQLKAFFDL
                     IQQWRRDDLLLAYHDRSDGGLFATLCEMAFASHCGLSVMLDTVAYDPFMNDVDGLDKR
                     PDALKGRFNDMLFKALFAEELGAVIQIRREDRSRITEPLRQAGLNYHFVGEPNDRDEL
                     RFWRNAKLQFAAPRAELHRIWSETSYQIARLRDDATCAQEEFDALAEAGNPGLSVVLG
                     FDPAEDIVAPFIATGARPKVAILREQGVNSQAEMAAAFARAGFAPYDVHMSDLQAGRV
                     HLEDFKGLAACGGFSYGDVLGAGQGWAKSILFNSRLRDQFEAFFQRTDTFALGVCNGC
                     QMMSNLAEIIPGGDCWPRFQRNRSEQFEARFVMVEVVDSPSILLAGMAGSRMPIVVSH
                     GEGRAVFAGIDAQDNARVALRYVDNTGAVAERYPFNPNGSPAGITGLTTPDGRFTIMM
                     PHPERTARTVQMSWHPAGLGEDSPWLRMFRNARRWVG"
BASE COUNT         5533 a        10266 c         9265 g         5323 t
ORIGIN      
        1 tgggggaaca ccaccacgtc gcgcaggggc aacaggggca gttcgatctg ttcttcgggg
       61 aggtcaagcg cgctcgacat gattattcct ttaaaggtac taaggcccca tatggggccc
      121 tagcgacatc aattcaagca gaccagcgga aaatctgcgg gatgtcagtt ggagccggag
      181 accttgggtt gatcggcgta gatcaggatg ggtttcgctg caccgtcgat cgtgccctcg
      241 tcgatgacca ccttggccac attttccatc gacggcagtt cgtacatggt gtcgaggagc
      301 gtgctttcaa ggatggaccg cagaccacgg gcgccggtct tgcgtttcag ggccttgcgg
      361 gcgatggcct gcagcgcgac aggacgcacc tccagttcga ccccttccat cgagaacagt
      421 ttctggtact gcttgatgag ggcgttcttc ggttcgatca ggatctggat gagggcttcc
      481 tcgtccagct cctgcagggt cgccaccacc ggcagacggc caatcagctc ggggatgaga
      541 ccaaacttga tcagatcctc gggctccact tcccgcaagg tcttggagac atcggcagtg
      601 gcgcggctct tcacctcggc gccaaagccg atcccgacct tctcggtgcg gttgcggatg
      661 accttttcaa gtccgtcgaa ggcgccgccg cagatgaaca ggatgttggt ggtgtccacc
      721 tgcacgaaat cctggttcgg atgcttgcgt ccgccctggg gcggcaccga ggcgatggtg
      781 ccctcgatga gcttgagcag ggcctgctgc acaccttcgc ccgacacgtc acgggtgatg
      841 gacgggttgt cggacttgcg ggagattttg tcgatctcgt caatgtagac gatgccctgc
      901 tgggccttgt ccacctcgta gtcgcacttc tgaagcagct tctggatgat gttttccacg
      961 tcctcgccga cgtagccggc ttcggtgagg gtcgtggcgt ccgccatcac gaagggcacg
     1021 ttgaggagac gggccaaggt ctgggccagc agggtcttgc cggaaccggt gggaccgacc
     1081 aggaggatgt tgctcttggc cagttcgact tcgtcgtggg acttgctcag gtgacgcaga
     1141 cgcttgtagt ggttgtagac cgccaccgac aggatgcgct tggcagatcc ctgcccgatc
     1201 acgtactgat cgaggattgc gcagatctcc ttgggcgacg gcagttcgcc cttgacgccc
     1261 ttggcaccgg tatcggcagt aatttcatcg cggatgatgt cgttgcacag ctcgatgcac
     1321 tcgtcgcaga tgaacacgga aggtccggcg atcagcttgc ggacctcgtg ctggctcttg
     1381 ccacagaacg agcaatacag gagcttctcg ccgctcgttt tcttgtcagc cattactgcc
     1441 ctttctctct gttacgtcga aatggagtac gtcggtctca ggcgacatcc gcgcgggagg
     1501 tcagcacctt gtccaccaga ccgtagcgca tggcttcttc ggcggacatg aagttgtccc
     1561 ggtcggtgtc cttctcgatg gtttccaccg gctgcccagt atgcttggcc agcatgtcgt
     1621 tgagcttggc gcgcagggac aggatctccc gggcatggat ttcgatgtcc gaagcctgac
     1681 cctggaagcc gcccagcggc tggtggatca tgacacggga attgggcagc gagaagcgct
     1741 tgccctgggc gccggccgcc agcaggaagg cgcccatgga cgccgcctgg ccgacgcaca
     1801 gggtgctcac gtccggcttg atgaactgca tcgtgtcgta gatcgccatc ccggcggtga
     1861 cggaaccgcc gggggagttg atgtagaaga agatgtcctt gtcgggattt tccgactcga
     1921 ggaacagcaa ctgggcgacg atgaggttgg cggtcacgtc ggtgaccggt ccgaccagga
     1981 acaccacccg ctccttgagc agacgcgagt agatgtcgta ggaacgctcg ccgcgaccac
     2041 tctgctcgac caccatcgga atcaggccga ggccgaccgg atcccagttg ctacgcgaat
     2101 cggggggtgt catgaagttc atagatcgtc gcctttcgcg gccttacgcc gcattgccca
     2161 tcagttcttc gaaagaggtt tccttgtcgg tcgccttggc gttacccacc acccaggcca
     2221 ccacgttgtc ttcgaccacc agggcttcag cctgcgccag acgttccggc tgggcgtagt
     2281 accagctcac cacttcgctc gggtcttcgt agcttgccgc aaagtcttcg atgatcgcgc
     2341 gaatttgttc cggcttggcg aagagttcct tgctgcgcac cagttcggac atgatcagcc
     2401 ccagcttgac gcggcgcacg gcctggtcgg tgaaccagtc ggtggagatc ggcagatcct
     2461 tggttttcat gccgcgggat tcaaaatcct tgcgggcgtt gtcggcgagc tgctgggatt
     2521 cctgctccac cagcgccttg ggcacttcga tgggatgcgc gtcaagcagc acgttcatga
     2581 cctgctcctt gatgcgggcc tggatgcgac gcttcacttc gcgctccagg ttggccttca
     2641 cttcctcgcg catcttgctg gtgtcgccgt cggcgatacc cagggacttg gcgaattcgc
     2701 cgtccacttc aggcagcttg ggctcggcaa cctgcttgac ggtgacctcg aactggacgg
     2761 cttgacccgc cagatgggag gcgtggtagt cgtcggggaa ggtcatgtcg aaggtcttgg
     2821 actcaccggc ggacaggccg accacagcgg tctcgaaatc cttgagcatc tggccggcgc
     2881 cgatcacgaa ggggaaatcg gtcgccttgc cgccgtcgaa ctcgacaccg tccttgcggc
     2941 cggtgaagtc cacggtgacg cgatcgccat cctgagccgc gcgttcggcc ggcacaaatg
     3001 tggtgcgctg cttgcgcagg acctccaggg tcttatcgac ttcggcttcg gtcaccgaca
     3061 ggatcggccg ctcgacttca cgggcgctca gatcgccgac caccacttca ggataaacct
     3121 cgaacaccgc gctgaattcc agcgcttcgg cagacgccgc atccttcggt tcgatgcgcg
     3181 gatagccggc gatgcgcagg ttctgggcac ggatctgttc ggcgagcacc cgctcgaccg
     3241 cggcgccgac ggcctcggag cgggcctggg agccatagga ctgcgccacc atccggaacg
     3301 ggaccttgcc cggacggaag cccggcatct tcacggtgcg ggccatctgc ttgagacggg
     3361 cctccacatc cttttcgata tcggccagcg gcacggtcat gtcgatacgg cgctccagcg
     3421 cgctcacggt ttcctggttg gtctgcatga aaagtttcct gatcggtcta cacaaagacg
     3481 catcacgagc aggcaggtga gacaagagca atactcgtgc cgtcctgatt tcccactcat
     3541 gatgcggtcg gaaaaatctt tagtctaaca cagagacaag gacaccttgc cttccgtcca
     3601 aaacgttgct tggatgaaag ggaaaatttc tccttgcagg ttcaaaggaa ctggcattct
     3661 gtccgggagt cggataacca tgcaggccag accgccgcaa taacacgtaa cgacgggcat
     3721 ttcggggcga tcccgccgct caagattgcc cgtcccccgc cgttggagca cctcaccgcc
     3781 acacgcagca gatcgtcaga aacccgcgca gcccgccagg gcgccccgat ccaggagacc
     3841 cagatgagca tcttcaccag cttccagtcc cgattcgaat cggcccggga ggaggaaatg
     3901 tcggtccagg aatatctgga tttgtgcaag gctgatccgc ttgcctatgc cacttcggcc
     3961 gagcgcatgc tccaggccat cggtgagccg gaactggtcg acacccgcct cgaccctcgc
     4021 ctgtcccgca tcttctccaa caagatgatc aagatctatc cggccttccg ggacttctac
     4081 gggatggagg aggccatcga gaacatcgtg tcgtatttcc gacacgcagc tcaggggctg
     4141 gaggaaaaga aacagatcct gtatctcctc ggcccggtgg gcggtggcaa gagctccctc
     4201 gccgaaaagc tcaagtcact ggtcgagcgc gtccccttct atgccatcaa gggctcgcct
     4261 gtgcatgaat cgccgctggg gctgttcagc gcgggcgagg acggcgagat cctggaatcc
     4321 gactacggca tcccgcgccg ctatctcaac accatcatga gcccgtgggc ggtcaagcgc
     4381 ctgcacgagt tcaatggcga catcaccaag ttccgggtcg tcaagctgcg cccgtcggtg
     4441 ctgcgtcagg tcgccgtcgc caagaccgaa ccgggcgacg agaacaacca agacatctcg
     4501 tccctggtcg gcaagatcga catccgcaag ctcgaacagt tctcccagga cgacccggat
     4561 gcgtactcat acagcggtgg cctgtgcctc gctaaccggg gcatgctcga attcgtcgag
     4621 atgttcaagg cccccatcaa ggtcctgcac ccgctgctca ccgccaccca ggaaggcaat
     4681 tacaagggca ccgaaggttt tggcgcgatt cccttcgacg gcatcgtgat ggcccactcc
     4741 aacgaatcgg aatggacggc cttcaagaac aaccgcaata acgaagcctt cctcgaccgc
     4801 atctacaccg tcaaggtgcc gtactgcctg cgcgtgtccg acgagatccg catctacgac
     4861 aagctgctaa ccaacagctc cttgtccgag gccccctgcg cgcccgacac cctgcgcatg
     4921 atggcccagt tcgccgtgct gtcccgcgtc aaagaaccgg agaactcctc catctactcc
     4981 aagatgcgtg tctacgacgg tgagaatctg aaagataccg accccaaggc caagagctat
     5041 caggagtacc gtgattacgc cggcgtcgac gaaggcatga ccggtctgtc cacgcggttc
     5101 gccttcaagg tgctgtccaa ggtcttcaac ttcgatcacc gggaagtggc ggccaacccg
     5161 gtgcatctga tgtacgtgct cgaacagcag atcgcccagg agcagtatcc gcccgagaac
     5221 gaggccaagt acctgggctt catcaaggag ttcctggcgc cccgctacgc cgagttcatc
     5281 ggcaaggaaa tccagaccgc ctacctggaa agctacagcg aatacggcca gaacatcttc
     5341 gaccgctact gcacctacgc cgacttctgg atccaggacc aggaataccg cgaccagaac
     5401 accggcgaga tcctcgaccg caacgccctc aacgacgaac tcgaaaagat cgagaagccg
     5461 gccggcatca gtaacccgaa ggatttccgc aatgaggtgg tgaacttcgt gctgcgtgcc
     5521 cgcgccaaga atgccggcaa gaacccgagt tggaccagct atgaaaaact gcgggcggtg
     5581 atcgagaaga agatgttctc caacaccgag gatctcctgc cggtcatcag cttcaacgcc
     5641 aaggccagcg gtgacgaaca gaaaaagcat cagaacttcg tcaaccgcat gatcgagaag
     5701 ggctataccg agaaacaggt gcgcctgctc tgtgagtggt atctgcgcgt ccgcaagtcg
     5761 tcgtgattcc cagcgccctg cccgccgaca ggcgggcgga gcacgtgcgg cacccaccgg
     5821 gaggcttcca tggtccgaat cgtcgaccgg cgactcgaca gcaagaacaa gagcgcggtc
     5881 aatcgccagc gcttcatgcg ccgcttcaag aaccagatcc gtcgggcggt gtccgacgcg
     5941 atcgcgggac gctccatcaa ggatctcgac aacggcgaaa gtgtatccat ccccgccaag
     6001 gatctggcgg aaccccagtt ccaccacggc cgcggcggca tctgggaaac catctacaca
     6061 ggcaacgacc agttctcgtc cggtgaccag atcaaccgtc cccagggcgg cggaggcggt
     6121 ggcacaggta aaggcaaggc cagcaacgaa ggggaagggg aggacgattt cgtcttcaac
     6181 ctgtcgcggg aagaattcct cgatgtcttc ttcgatgacc tggccctgcc gaacctggtc
     6241 aagacccagc ttgcccgcat ccacgaatac aagatccagc gcgccggctt caccaacgac
     6301 ggcacgccgg ccaacatcaa cgtggtgcgt tccatgcgcg gcgcccttgg ccgccgtctg
     6361 gccctggctt cgccctacgc cggcaagctg cgcgaactgc agaagcagct cgacgaactc
     6421 ctcgaaaccc gaccggacga cgatccggac gtggaaaaac tgcgccacga catcgccgtc
     6481 ctgcgcgcca agatcgaggc gattccgttc atcgacacct ttgacctgcg ttacaacaac
     6541 cgggtgcgca tccccaagcc cacgacccag gccgtgatgt tctgcatcat ggacgtctcc
     6601 ggctccatgg acgaggagaa gaagtccact gccaagcgct tcttcatgct gctctacctc
     6661 tttctcaccc gcacctacga gcacatcgag gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nngcttcttc atgctgctct acctctttct cacccgcacc tacgagcaca
     6841 tcgaggtggt cttcatccgc caccacacgg tggccaagga agtgaacgag gaggatttct
     6901 tccactcccg cgagtccggc ggcacggtgg tgtccagcgc cctcgagttg atgcgtgaca
     6961 tcctccacga gcgctatctc aatggcgcct ggaacgtcta tggcgcccag gcttccgacg
     7021 gcgacaactg ggaaaacgat tcgccccatt gccgggagct gctggccgaa accatcctgc
     7081 ccttcgtcca gtacttcgcc tacatcgaga tcacgccggg cgaaccccag aacctgtggc
     7141 gcgaatacga aaagctgacc ggcgcgaaat ccaacttcgc gatgcagcac atcgaaggcc
     7201 tgccggacat ctaccccgtg ttccgcgaac tcttcaagaa gaaactcgca tgagcgccag
     7261 acgacccaaa cgcaagaagg ccctccccgc tcccggcgaa tggactttcg acctcattga
     7321 gacctaccac gccgagatcg accgggtggc gcgggatttc gggctcgaca cctaccccat
     7381 ccagctcgag atcatcacct ccgagcagat gatggacgcc tattcgtccg tcggcatgcc
     7441 cgtcaattac caccactggt ccttcggcaa gagcttcctg cacactgaaa agagctaccg
     7501 ccgcggacag atggggctcg cctacgagat cgtcatcaac tccaacccct gcatcgccta
     7561 cctcatggaa gagaacacca tgacgatgca ggccctggtg atcgcccacg cggcctacgg
     7621 ccacaacagc ttcttcaagg gcaactatct tttccggcag tggaccaacg ccgacgcgat
     7681 cattgactat ctgctcttcg cgcggaattt cctgggcgaa tgtgagcagc gccacggcga
     7741 agaagccgtt gagctgctgc tcgactcctg tcatgccctg atgaacgtgg gcgtggatcg
     7801 ctacaagcgc cccgccaagc tctccctcga gaaggaacgc acccgccagc gggagcgcgc
     7861 cgaatacctg cagagccagg tcaacgatct gtggcgcacc ctgcccattc agcagatcaa
     7921 ggcccaggcc gtggaggagc gccgcttccc gcccgagccc caggagaacc tgctctactt
     7981 catcgaaaag aacgcccccc tgctcgaacc ctggcaacgg gaagtcgtgc gcatcgtgcg
     8041 caaggtgggt caatactttt tcccccagcg ccagacccag gtcatgaacg aaggctgggc
     8101 cacctactgg cactacaccc tcctcaacac gctgtacgac gaaggcctgc tcagcgacag
     8161 cttcatgctg gaatttctcc actcccacac caacgttgtc tatcagccgc gctacagcga
     8221 accctggtac cggggtctca acccttacgc cctgggcttc gccatgtggc aggacatccg
     8281 gcgcatctgc gagcacccca ccgacgaaga tcgcgcctgg tttcccgata tggctggcag
     8341 cgactggcgc gagaccttcg atttcgccat gcgcaacttc aaggacgaga gcttcatcgc
     8401 ccagttcctg tctcccaagg tcatgcgcga cttccgcctc ttcgccatcc tcgacaacga
     8461 caaggacccc aagctcaagg tctccgccat ccacgacgaa agtggctacc ggcgcgtgcg
     8521 tgagatcctc tcggaccagt acaacctcgg gagccgcgag cccaatatcc aggtgtggaa
     8581 cgtggacctg cgcggtgacc gctccctcac cctgcgccac caggcctaca tgcgtcgacc
     8641 gatggggggc gacgccgagg aggtcgtcaa gcatatcggc cgtctgtggg gtttcccggt
     8701 acgcctggaa ggcgtgcagg acgacggtac ggtccagaag ctccatgaat ggtcgccgaa
     8761 acacgctcag gacgcttgat cgccggaacg cggaacccgg ccactgcgcc aaccgccatc
     8821 ccgatgatcc ttagcgtgcc gcaggcgggt aatatatccg gatgtccgcc gacgactttc
     8881 atcgcatcct cggagtcaaa cccggcgctt cggccgccga gatcaaacgc gcctatcgcc
     8941 gtcaggccat gcgctggcac ccggaccgca atgccgatcc cgacgcccac gaccagttca
     9001 agcgcatccg cgaggccttc gagcgcctga gcgtcggcgc ttcgcaggtc gacaacgacg
     9061 gcgacgcgga caccccaccg cccgccgcgc cccgtggcga ggaccgccgc gtcgaagtca
     9121 ccctcgatct tacggaagcc gcactcggga cgaccgtgga cgtcacggtc gaagggcgca
     9181 catcctgtac cgcctgtggc ggcagcggcc aacgcagcta tgggcgtacc accatgtgcg
     9241 gcgcctgcca cggcagcggc cgtgtccgca gcaagaatgg gctggacaac tgtacgcagt
     9301 gttccggcaa aggctacttc accgaccgcc gctgccccga ttgcgacggc cgcggctggc
     9361 atccatccga gcggcgcatc gaagtcacgg taccgccagc gctattgccc ggagatgaac
     9421 tccgcgtacc cggccaaggc ggccctgcgc ccgaagccgg cacaccgggt gatctctaca
     9481 tcaccgtacg ggcccgacct caccccctgt tccgtctgga cgggcggaac gtgcatctga
     9541 cggtcccggt cagcgtcttc cgtctgctgg cgggcggtga gatccttgtg cccaccctca
     9601 gcgggctcga aaccgtgcag cttcccgaag gcgccggtca gcccgaactg cgggttacgg
     9661 gccacggctt tccggcccgc ggcaagcgcc ccgccggcga cctgcaccta cacctcaaga
     9721 ccgaactgcc ggcctatctg agccaggaac agaaggccat gctcgaaatg gccgagcagg
     9781 ttctgcttca ccacctggac acccaaagcc cggctcttgc cctgtggaaa caggtgcttg
     9841 aacagcaccg ctgaacccgg cgcgccactc cgccgaacat cgccggcccc cttcccggct
     9901 gccacccatg cccccgaaaa cgcccgaact tctcgccccg gccggatccc tcgcgatgct
     9961 ggacaccgcc ctcgcctttg gtgccaccgc cctctacgcg ggccagccgc gctactccct
    10021 gcgggtgcgc aacaacgact tcggcgacct gcccatcctc gcggaaggca tccgccgcac
    10081 ccatgcgatc ggtgcgcgct tctatctggt ggccaacatc tatccgcacg gcgccaaggt
    10141 gaagaccttc ctgaaggaca tcgcgcccgt catagccctc gcccccgacg cgctgatcat
    10201 gtccgatccc ggattgatcc tcctcgtgcg ggagcacttc ccccacatgc ccatccacct
    10261 gtcggttcag gccaacaccg tgaacgcggc cgccgtgcgc ttctggcgcc aggcaggcat
    10321 cagccgcgtg atcctgtccc gcgaactctc cctcgatgag atcgcgtcga tccgccagga
    10381 ctgccccgat accgagctgg aggtcttcat ccacggcgcc ctgtgcattg cctattccgg
    10441 gcgctgtctc ctgtcgggct acttcaacca tcgggatccc aatcagggca cctgcaccaa
    10501 tgcctgccgc tgggagttca agacccacgg cgcacgtacc tgcagcgccg gtgatgtgca
    10561 tctgctcgaa gagccccaga agcgcgccgg cgaatacatg cccattgagg aagacgagca
    10621 cggcacctac atcctcaact ccagggatct gcgcgcgatc gagcatgtgg aacggctcgt
    10681 cgagatgggc atcgactcct tcaagatcga aggtcgcacc aagagcccct attacgtggc
    10741 ccggaccgtt caggcctacc gtcaggccat cgacgatgcc gttgcgggac ggcgtcttga
    10801 tcccgccctc ctcggccagc tcgaaggtct ggccaaccgc gggtataccg atggctttta
    10861 ccgtcgtcac accccccatg caacgcagaa ttacctgcgc ggccactccg aatcagggcg
    10921 tagtctgtat gtgggtgatg tggtggcctt cgatgctgcc cgcggcctcg cggaaatcgc
    10981 ttcccgcaac gccttttcgg tcggagaccg gatcgaactg attcagccgg ccggcacacg
    11041 cgaaatccgc atcgaccgtc tcgaagatgc agacggtgcg ccacttgaac gcgtgcccgg
    11101 cagcgaccac cgggcctgga ttccactgcc gcccgattgc gtcggtgcct ttgtggcgcg
    11161 ctttatacac ccctgaacca agaaccttgc ataaggcgct gaaagcgttc aaaatatagc
    11221 gttttacggg tggccaaatt caccacccac gtactttaag gaaccgtgat gaaagccgat
    11281 acccatccca aatacgccga agtggaagtg acctgctcct gtggcaacgt ctttaccacc
    11341 cgttccacgc tgggcaaacc tcagctgcat gtggaagtgt gtgcagcctg ccaccccttc
    11401 tacaccggta agcagaagat cgttgacacc gcgggtcgcg tcgagcgctt ccgtcagaag
    11461 tacggtaacg ttcaacgtct gggctgattc ccggcgacga ccgcaaaagg cagccgaggc
    11521 tgcctttttt attgccccca agactccgca ccgatgcctg ttcctcccaa gtctgccctt
    11581 ttgcccacgc ctctgaccgg ctggccgctt gcagtcctga tcggcgtcta cctgcttgtc
    11641 ggtatgggcc atgttccctg gcggggcgac gacctgacct atcttggtcc gatccacgcc
    11701 attctcaccg atggctcgtg gctgatcccg agcattgccg gcgagccctt ctacgaatat
    11761 ccacccctgt attactggct tggcgccgtc ctcgcacgcg cattcggatg gctgatgccg
    11821 gttcacgatg ccgcacgtct ggccagcagc ctgtgcgctg ccgccacact ctactggacc
    11881 ggtcttgcgg cacggcgact ttatggcgac aacacgggtg ctccggcgat cctgctcgga
    11941 cttggcagcc tcggactggt ggttcacgcc cacgaaacgc aacctctcct ggcccaactg
    12001 gcgggcatgg ccctgtgtta cgccggcctt gcagacatgg tcaggcggcc ttgggccggc
    12061 gcacgccagg caggaatcgg cgcaggcctg acctttctgg ccggcggact gcctggcatg
    12121 ttcgtcaccc tgccgctgtt ctttgccatg ccgctcctct gcgacaactg ccggaatggc
    12181 gccgtgccgc gggccctggc aggagggctt gtcgttgccc tgctgctggg cggtagctgg
    12241 cccgcactcc tgggatggct ccaccccgaa gcccttgcca gctggtggaa cgacgaactg
    12301 gccgccagcc tgccccacgg taatcacctg caggccctcg gaaagatgat cccgctgctc
    12361 ggctggttcg cgtggcccct atggcccatc gcgggctggg ccctgtggaa gaaccgtcgc
    12421 gccttgaaca ccccccctgt cgccctgccc atcatcgcaa gtcttctcgc gatggtggcc
    12481 gtcgtgacga cgggcccgct ccgtcccgcg agcaccctgc cgatcctgcc ccctctttgc
    12541 ctgctggcgg ccctgggcgt cggcaccctg cgtcgcggcg ccgccaatgc cctcgactgg
    12601 tttgcgatca tggcttttgc gttcttcgcg atcctggtgt ggatcggctg gtccgcgctg
    12661 tattttggct ggccgcccgg gctggggcgc cagttggcac gcctggcacc cgatctggta
    12721 cgttccccgt cgaccatcgc tgccgtgacc gggatgttcc tgagcctcgc gctcttcgcg
    12781 ctaccggtgc tcacaacccg ctctacgctc cggggggcta ccaactgggc catcggcatg
    12841 acgctgctct ggtgcctggc ggtcgcattg tggcaaccgt ggttcgagca caccaaaaac
    12901 tacgcgccgg tcgcccaggc gctggcccgc gaactggagc tccaccctgc cccttgcatc
    12961 acgcgcaagg gactgaacga cacccagcgc gcggccctcg actattttaa cggcataaaa
    13021 accattcatc attctgccga ccaagactgt ccggcatttc tcgtatatag tgccgacggc
    13081 ttgccgtatc gcccacccgg cgactggcgg acaagctggg aaaagcgcct cggtggaggg
    13141 cgcaagacgg agcttttcag actgtatcgg catgactgac ctgacgcctt ttgatctcga
    13201 cgcctcggac gcgggaacat ccctcagccg cctggcttcc gcaccagaac tgacccatct
    13261 gcgcgccctt ttctcaaagg accctgcgcg cttccaggcc ttcagctgtc gctacggcga
    13321 atggctgctc gattactcac gtcagcggat ggacgccgcc atccggtccc agcttctggc
    13381 gctggccgag gaatcacggc tccgtcaagg catcgatgcg atgctgtccg gcgccattgt
    13441 caatgtcacc gaacagcgcg ccgtactcca ttgcgcagcc cgcctctcgc cccacacccc
    13501 cctgaacgcg cggaatcaga tcgaagagac gctggcacgc gtgatggccc tggtcgaccg
    13561 gattcatgcc ggatcattga ccggaagccg cggaatgccg atccggcatg tggtgcatct
    13621 gggaatcggt ggctccgacc tgggcccgcg cgtgctttcg gaagccttct ccactgccta
    13681 tccgcggcgt ctcaaagtgt ctttcgcggc caacgtggat gcgtccgaaa tccggtcgat
    13741 cctcatcgac gcgactccgg aggaaaccct cttcgttgtc gcgtcgaaga gcttcacgac
    13801 ggctgaaacc ctcagcaacg cgcgtatcgc ccgtcaatgg ctgacggatc agcttggacc
    13861 cgatgccgat gtccgcgccc actttgcagc catcagcaac aacgtccatg ccgcgtcctc
    13921 cttcggcgtc cctgaggaac gcatcttccc cttgcccgaa tgggtcggcg gacgcttttc
    13981 ggtgtggtcg gctatcggta tcccgatcat gctcgaattc ggaactcccg tgtttctcga
    14041 attcctcgcg ggtggacggg acatggacgc gcatttcgca acggccccat ttgactcgaa
    14101 tctcccggtg ctcgcggccc tcatcggcct ctggaacgcc agctacctgc gcatcaactg
    14161 ccacgccgtg ctgccctact gtcacgccct gcgcagcctg ccggcctacc tgcaacagct
    14221 tgagatggag agcaacggca agcacgtccg gcgggacggt agtgccctgt cgtacccgac
    14281 ggccccgatc ctgttcggcg gcgctggcac ggtcggccag cacgcctacc accagctctt
    14341 ttatcagggc accgaaaccg ttgccctgga tttcatcgtt ccgattccgg acaccccgac
    14401 gcctgcaacc cgcagcctcg tgggcaacgc gctggcccag gccgcagcgc tgatgcacgg
    14461 acgcacagag gatgaagcgc gtgcagaact cacgcaacgt gggctcgcac aggatgaagt
    14521 cgagcgtctc gcacctcacc tggtatgccc gggcaaccaa cccagcagca cgctgctgat
    14581 gccagccctg acccccttca cggtcggtca gctcattgca ctgtacgagc acaaggtgtt
    14641 tgcccagggc tggatgtggg gcctcaacag cttcgaccag tatggcgtcg aactgggcaa
    14701 gcaactcgcc cgccgtcttg aatccggcga catgtccgat gcggacagct ccacccaggg
    14761 cctcatggcc gacatcgcca gacgctggga accggccaag gaatgacgcg gccgtacgac
    14821 accaaataaa aaggcgggtc cgacccgcct ttttttcgca cgcgtgtcac gtcattgcgg
    14881 agtcttctgc ggcacctgca catacacctc gtccggcttg atcaggccca attcgaagcg
    14941 cgcacgctcc tcgactgcct cgtagccgga cttcagatcc cgcacttccg cttcgagccc
    15001 cgtgttgcgc tgctcgagct tgcgattgcc ttccttctgc tcggcgagct gctgctccac
    15061 ttcccagacg cgcaaccagc cgcccttgcc gatccacagg ggatactgca aggcggcgac
    15121 gaggaggacg aggatcagca gcggccagcg catgggagat gctccggatc ggcctcacgg
    15181 ccgcgccgga gctgaaccga tcaacgggtc cgcaggttgt agaacgccga cagaccgggg
    15241 tagaaggcgg tatcgcccag atcttcttcg atgcgcagca gctggttgta cttggagatg
    15301 cgatcggagc gggacaggga gccggtcttg atctgcatcg cgttgagacc cacggcgatg
    15361 tcggcgatgg tggagtcctc ggtctcgccg gaacggtggg agatcaccgc agtgtagctg
    15421 gcgcgcttgg ccatctcgac ggcggcgaag gtttcggtca gggtgccgat ctggttgatc
    15481 ttgatgagga tggagttggc gatgcccttc tcgatgcctt ccttgaggat gcgggtgttg
    15541 gtgacgaaga ggtcgtcgcc gacgatctgc accttcttgc ccagacgctc ggtcaggatc
    15601 ttccagccgg cccagtcacc ttcggccatg ccgtcttcga tggagatgat ggggaacttg
    15661 tcggccaggg tggcgaggta atcggtgaag ccctcggaac tgagggacag gccttcgcca
    15721 tgcagttcgt acttgccatc cttgaagaac tcggacgcgg cacagtcgag ggccagcacc
    15781 acgtcgcggc ccggctcgta gccggcgttg gcggtggctt cgaggatcat gttgagggct
    15841 tcgtcggtgc ccgacacatt gggcgcgaaa ccgccttcgt cacccaccgt ggtggggtag
    15901 cccttcttgt cggtgagctt cttcaggtga tggaagatct cggcgccgca gcgcagggct
    15961 tcgcggaagc tcttggcgcc gaccggcatg atcatgcatt cctggatgtc cagggagttg
    16021 ttggcgtggg caccgccatt gatgacgttc atcatcggca cgggcataga catgccgccg
    16081 gagccgccga agtagcggta caggggcaga ccggcctctt cggccgccgc cttggccacg
    16141 gccatggaga cggccagcat ggcgttggcg cccaggcgcg acttgttctc ggtgccgtcc
    16201 agttcgatca gggtccggtc gaggaaggcc tgctcttcgg catccagccc gaccagcgct
    16261 tcggagattt cggtgttgac gttctcgacc gcttgcagca cgcccttgcc caggtagcgg
    16321 gcggcatccc catcgcgcag ctcgatggct tcgcgggatc cggtggatgc accggacggc
    16381 accgccgcgc ggcccatcac gccggattcg agcaggacat cggcttcaac cgtgggattg
    16441 ccgcgggaat ccagtacctc gcgggcaatg acatcaacaa tggcgctcat gaacttcctt
    16501 tcgagtgtgc aaatggcgat ataacgggtg cggccgtcgg accgcgttca aatttgaagg
    16561 atggcggggc ggggctgcgc tccatcaggc ctgcgtttcg atgaagccct gccccttgac
    16621 cagcgcatcg atatccttga gggtcttcag cagcgccttg aggcgcggca atggccacgc
    16681 gttgggaccg tctgacagag ctttggccgg gtccggatgg gtttccatga acaggccggc
    16741 gacgcccacg gccaccgcgg cccgcgccag gaccggcacg aactcccgct gaccaccgga
    16801 cgaggtgccc tgcccgccgg gcagttgcac cgaatgggtg gcgtcgaaca ccaccgggca
    16861 gccggtgtcg cgcatgatcg ccagggaacg catgtcggac accaggttgt tgtagccgaa
    16921 ggatgcgccc cgctcgcaga ccatgatcgt gtctgccccg ccattggctt cgcgggcctt
    16981 gtccaccaca ttcttcatgt cgccgggcgc aaggaactgg cccttcttga tattcacagg
    17041 cttgccggaa gcggccaccg cgtggatgaa gtcggtctgg cggcacagga aggccggagt
    17101 ctggagcaca tccaccacgg ccgccacggc cgggacttcg tcttcggtgt ggacatcggt
    17161 gagcaccggc acgccgaccg tggcgcgcac gtcggcgagg atctcgagcc ccttctccat
    17221 gcccatgccg cggaaggact tgccggaact gcggttggcc ttgtcgtagc tggacttgta
    17281 gatgaagggg atggccagct cggcgcagat ctccttgagc gccgacgcgg tgtccagcgc
    17341 catctcgcgg gactcgatga cgcacggtcc ggcgatgagg aaaaacggct tatccagacc
    17401 gacatcgaat ccgcagagtt tcatggggtt tcctttcagc cggcttgctg gtgggccagg
    17461 gccgccttga cgaatgcagt gaacagcgga tggccctgac gcggattgga ggtgaattcc
    17521 ggatggaact gacagcccac aaaccacggg tgggcgttcg ggccttccgt cggcagctcg
    17581 atcatctcgc acagatcagt gcccggcgcg cgtccggcga cccgcagacc ctgcgcttcc
    17641 agacggctca acaggctatt gttcacttcg tagcgatgac ggtgacgctc ggtgatctcg
    17701 ggccgaccat agacttcccg cgccaatgaa ccttcggtca agcggcagac ctgaccgccc
    17761 agacgcatgg tgccgcccag gtcggaatcc tcgccacgct tttcaagctt gccggaagcg
    17821 tccagccact cggtgatgag gccgatcacc gggaattccg tgtgcttgtc gaattcagtg
    17881 ctgtgggcac cgttcatgcc ggccacatca cgggcgaact cgacgacagc gagctgcatc
    17941 ccgaggcaga tgcccaggta aggcaccttg ttctcgcggg cgtagcggat cgcggcgatc
    18001 ttgccttcgg tgccgcgccg accgaacccg ccgggcacga gaatcgcatc catgcgcttg
    18061 agcacgtcgc acccgctctt ctcgatgtcc tcggagtcga tgtagtggat gctcaccttg
    18121 ctgcgggtgt gcatgcccgc gtgattcagc gcctcgatga gggacttgta ggactcggtg
    18181 agatccacgt acttgccgac aaaggcgatg tccaggtgat gctgggggtt gttgagcgca
    18241 tcgatgaggc gctcccacac agacagatct gccgcgcggg ccaggatgcc cagcttgtgg
    18301 cagacgatct cgtcgagcat ctggtcatgg agcatgcccg ggatgctgta gatggaatcc
    18361 gcatccagac actcgatgac cgcttcgggc atcacgttgc agaacagcgc aatcttgcgc
    18421 cgctcgtcgg cggggatgga acggtcggcg cggcacagca ggatgtcggg ctggatgccg
    18481 atctcgcgga gttccttgac ggagtgctgg gtcggcttgg tcttcagctc gcccgcggtg
    18541 gggatgtagg gcagcagggt caggtggatg aagcaggtgc cctgacgacc ttcctcgata
    18601 cccatctgac ggatggcttc caggaaaggc agcgattcga tgtcgcccac cgtgccaccc
    18661 acctcgacga tggccacgtc cgccccttcg gcgccgcgct tgatgtagag cttgatctcg
    18721 tcggtgatgt gggggatgac ctgaaccgtc ttgccgaggt attcgccgcg gcgttccttc
    18781 ttgatgacgg cttcgtagat ctggccggtg gtgaagttgt tgcgcttgcc catcttggcg
    18841 ctggtgaagc gctcgtaatg gcccagatcc agatcggtct cggcgccatc ttcggtcacg
    18901 aacacttccc cgtgctggaa gggactcatc gtgccggggt cgacgttgat gtaggggtcg
    18961 agcttgaggt gggtaacctt gatgccgcgg gattcgagaa tggccccgag ggaggcggcg
    19021 gcgatgccct tgcccagaga ggacacgaca ccaccggtaa cgaagacgta tttggtcatg
    19081 acacggggga cttctggaaa gagaaattat agccgatcca cccaccccgc tcaacggtgg
    19141 atgcctcccc agccgtcccc cgaaacgctg cccgtacgcc tccgtcagcg gatgaaattc
    19201 cggctgaccg tccggaattc cggtgccgtg cagcgctcca tccattcgaa cgccaccatc
    19261 tcgcgactca ccacttcgat accgtgggcc cgcatgcggg ccagggcaag ttccttgtca
    19321 gcaggctttc gcgacccgac cgcctcggcc acgacgaaca cctccttgcc ctgccaacgc
    19381 agcccgaggg cagtctgaag cacacagaca tgtgcctcgg tgcccgtcac aaccacctga
    19441 cgacgggcat cgaccggcgt gccgctcaga ctcccgtccg ccacacagga gaaggccgtc
    19501 ttgtacacca cggccgcgtc gcctgcagcc tcctgcaagg cggtcgccgt tggtccgagc
    19561 ccctgcggat attgctctga cagcacgacc ggcacgccca gttcacgggc gacacggacg
    19621 agccagacgc aatggtcgag tacggcggcg ccatcgtcga tcgcgggcaa cagacgctcc
    19681 tgaacatcca cgaccagcag gctggcgcaa cgacgatcca tcagcatggc ggactcctca
    19741 gttcaccagt tccgggcgcg accggaaatt gtccagggcg gcggggttgg cgagcgcctc
    19801 gaggttcttc accggttccc catgcacgac ggcacgcacg gccagttcga cgatcttgcc
    19861 gctgcgggta cgcggaatgt cgtccacctg gacgatgcgc gccggcacat ggcgcggcgt
    19921 cgcgttgtcc cggatggtct gacggatctg ttcgcacagg gcgtcgtcca ggacccgccc
    19981 gtcccgcagc ttgacaaaca gcaccacgcg cacgtcgccg ctgcccgctg gccactgctg
    20041 gccgatgacg agggattcga gcactgcgtc gcacttttcc acctggcggt agatttcggc
    20101 ggtgccgatg cgcacgccgc cgggattgag ggtagcgtcg gagcgaccat agatgatcag
    20161 ccccgcgtgg gcggtaatct cacaatagtc gccgtggcac cacacgtgct cgaaacgctc
    20221 gaaatacgcg gcacggtagc gactgccgtc cgggtcgttc cagaatccga cgggcatgga
    20281 cgggaagggc gcggtgcaga ccagttcgcc tttctccccc tgtattggcg tgcccgtctc
    20341 gtcgaaaacg tctaccgcca tgcccagccc gcggcactgg atctcgcccg gatacacggg
    20401 cagcgtcgga ttgcccagaa cgaagcacga gatgatgtcc gtcccgcctg agatggatga
    20461 caggcacagg tccgccttga ccgcccgata cacgtactcg aagccttccg gcaccagcgg
    20521 gctgcccgtc gagaacatcg cccgcaggga cgtgaggtcg tgcgtctccc gcggcgccac
    20581 cccgagcttg gcgatgccgt cgatgaactt ggccgacgtg ccgaagtggg tcatgcgctc
    20641 cgcctcggcg aaatcgaaca ggatgctgcc ctgccccacc atcggcgaac cgtcgtacag
    20701 cagcagcgtc gcgcctgaag ccaggccact caccagccag ttccacatca tccagccgca
    20761 ggtggtgaag tagaacaagc ggtcgccggg ccggacatcg ccatgcaggc ggagctcctt
    20821 gatgtgctga agcagggtgc caccggcact atggacgatg cacttgggca cccccgtcgt
    20881 gcccgacgaa tacaggatga acaagggatg ggcgaagggc agccggacga agggaatctc
    20941 cgtctccgcc aggaatggcc gcacgaattc gggcagcaga cgggcatgat ggatgtggga
    21001 gagatcgtgg ctcgcatgga cgtagggcac caccaccacc cgccgcaggg acggcagacc
    21061 atcggcgatg gcggccaggc ggtcgcgaca atccaccacc ttgccgttgt aataatagcc
    21121 atccaccgcc accagcacct tgggctcgat ctgcccgaag cggtcgagca ccccctgcac
    21181 gccgaaatcc ggcgacgcgg acgagaagac tgcgccgatg ctcgccgccg ccagcatgat
    21241 gacgatcgtc tccggaagat tcggcatcca cgccgccacc cggtcacccg cgcccacgcc
    21301 ctgctcctgc aaggccgcgg caaagtgcgc cacctgtcgg tacagatcgc cgtgggacag
    21361 ccggttcttg accttgtctt ctccccagaa caccagcgca tcggcgtcgt cgcggctgcg
    21421 caacaggttc tcggcgtagt tcaggcgggc atcgggaaac cagcgggctc ccggcatccg
    21481 ctcgccgtcg gcgagcacgg tgtcaccgcg cactccgcac acgtcgccgt cctcccagac
    21541 cgaggtccag aactcctctg gatgggcgac agaccaggca tgcaacgccg cgtagccatc
    21601 aagcggccgc tgccaacggg cagacgcgcg ctgcgcgaat ccggtgatca acgccgcggc
    21661 cacgcgctcg ggcgacggcg tccacagggg cgcggcagca atcggataac tcatggcgaa
    21721 actcctcttc ctcgaacggg aatggatcga cggaacaggc cctcaggcca ccgcggcacg
    21781 cagcgcatcc ggcaagggcg cggacttgcc ggtacgcgta tccatccaca ccaccttgca
    21841 gtcgcccgtg gcaaacacgc gttcatcacc ctccacgccc atctcgtacc aggtcatcac
    21901 gctgctgcgt cccggcgcgc cggcatacag acgcacgatc acggtggcag gatagttcac
    21961 cgggatcaaa aaggtacaac tcgcgttgat gatcaccggc gcggtctcgg cggtgggccc
    22021 cacggcaaat ccgaacgctt ccagccactc cacccgggcc tgctcgtaat agcggaaata
    22081 caccgtgtta ttgacgtgcc cgtagaaatc catgtctccc cagcgtacgg ggatgcgggt
    22141 ggtgagcaga agtttgcgat caggcgcaga ggcgggtgtg ttcatgggct gtctcctgtg
    22201 ggtttgctgg attcgttgtc gtggccaccc gcccatcacg cccaggcggg ggcacgagga
    22261 atttagcata cggtggcgta tgcccaacac accggagggc gtcgggcagg cttttttgtg
    22321 acgccgggat tgccccgtcc tgcgcccgga tgtacgctcg acgtctaacg aaaacaagac
    22381 ataccaaaac aaaaggaggg cagtatggaa cgcgattcca tggactttga cgtcctgatc
    22441 gtggggggag ggcctgccgg gctgtccgcc gcgatccgga tcaagcagat cgcagcagaa
    22501 aaggggcagg acgtctccgt ctgcctcatc gagaagggcg ccgaggtcgg cgcccacatt
    22561 ctctccggcg cggtggtcga cccccgcgcc ctcaatgaac tcttcccgga ctggcaggcc
    22621 ctcggcgcac cgctcaccac gcccgtgagc gaggaccggg taatcttcct gtccgaaacc
    22681 ggcggcttga aggtgcccaa cgccttcctg ccggacagtt ttcacaacgc cggcaattac
    22741 atcgtccgcc tcggcaatct ggccaagtgg ctgggggaac aggccgaagg cctcggcgtc
    22801 gagatctatc cgggctttgc cggcgcccag ttgctcaccg gtgacaacgg cgcggtgctg
    22861 ggcgtcatca ccggcgacat gggcctgctc aaggacggga ccgaaggccc caacttccag
    22921 ccgggcatgg agctgcgcgc aaaatacacg ctgttcgccg aaggctgccg tggccacctg
    22981 ggcaaggaac tcgaagcgcg cttcaatctg cgcgaaggcg tcgaccccca gacctacggc
    23041 atcggcctga aggaactgtg ggaagttccc gcgccccagc atgtgcaggg tcttgtggtg
    23101 cacaccgcag gctggccgat ggaggcatcc acctatggcg gcgggttcgt ctaccacctg
    23161 gaagacaatc tcatctccgt cggctacgtg gtcggcctca actacagcaa tcccttcctg
    23221 tcacccttcg aggaattcca gcgtttcaag acccatcctg agatccgcaa attccttgaa
    23281 ggcggcaagc gcctcgccta cggcgcccga gccatcgcgg ccggcggcct gcagagccag
    23341 cccaggctgg tcttcccggg cggtgccctg atcggtgacg atgccggctt cctcaacgcg
    23401 gcccgcatca agggcaccca cgccgccatg aagtccggga tgctcgccgc cgaggcggtt
    23461 ttcgaggccg tcaccgccgg tcgcgcccag gacagcctcg acgcctatcc cgccgccttc
    23521 cgcgagagct ggctgtattc cgagttgcac aagactcgca acttcaagcc ctacatgaaa
    23581 aaagggctgt ggatgggctc cctgctcttc ggtatcgacc agaaggtctt cggcggcaag
    23641 ctgccatgga ccctccacaa ccacgccgat cacaccgccc tgcgaccggc cgccgaatgc
    23701 acgccgatcg cctatcccaa accggacggc aagctgacct tcgaccgcct ctcctcggtg
    23761 ttcctgtcca acaccaacca cgaggaagac gaaccctgcc acctgcgtct ggcggatgcc
    23821 tcgattccca tctcggtcaa cctggcccgc tatgacgccc ccgagcagcg ctactgtccg
    23881 gctggcgttt acgagatcgt acgcagcgac gacggtgccg cgcgcctgca gatcaacgcc
    23941 cagaactgcg tccactgcaa aacctgcgac atcaaggatc cgacccagaa catcaactgg
    24001 gtcacgcctc aaggcggcga aggcccgatc taccagggca tgtagcgcgc ccgtcgtcca
    24061 gcgggcgtag caccctgcgc ccgccccacc tcaagtcatc atggggcagg ccgttagggc
    24121 ggatatcctt ttggtccgtc ccacgacctg ccaccatgaa gactgcccag atcgtcctga
    24181 acggatcctc cgtcagttcc agcctcgcga ccctcaccgc gatccagccc cacctcgtat
    24241 ttgtgttcgc cgacccgtcc tggtgcgtcg gcgaagaccg gctctcgccg ctcctccaag
    24301 ccctgacgcc cgcgcagctg gtcggctgct ccacggcggg cgaagtggcg ggccacggcg
    24361 ttcaggaaaa ctcctgcgtg ctgaccgcgg tccatttcga cgacccggcg ttcaccgtca
    24421 cccgcacgca cgtggacgac atgcgcgact cccgcgcagc cggtgaacgg ctggcggtcg
    24481 ccctggcgcc ccactcgcct cattccgtac tggtcttcgg tcctggggtg cgcctcaacg
    24541 gctcgtcact ggtggccggc atggcggcag gattgcctgg cgccaccaca ctggccggcg
    24601 gtctggcggg cgatgcgggg cattttcgta ccacctggac catcggaccg gatggcatcc
    24661 gcgagcggga cgtggtcgcc gtggggtttc acagcccccg cctgaaggtc tgccatggaa
    24721 gcttcggtgg ctggacgccc tttggacccg ttcgacgggt cacgcgggcc catgaaaaca
    24781 tcctgtacga actggacgga gagcgcgccc tcgatgtcta tcggcgctac ctgggcgact
    24841 acgccaagga cctcccggcg tccggcctgc tgtttccttt tgcaatgctc aacggtgacc
    24901 gggatgaaac cggtatcatc agaaccattt tgggtatcga cgccgaggcg ggcagcctgt
    24961 tgctggccgg agaaatcgat ccgcacggct acctccgtct catgcacgcc agcacggatc
    25021 gcctggtgga cggcgccgcc gaagcggccg cccggatcgg taaagcgccc cagcgcagcg
    25081 atggactggc cctgctggtg agttgcgtgg gccgcaaact ggtcatgggc gaccgggtgg
    25141 acgaggaggt cgaagccgtc gcccgtgtcc tcggtccgac aagttgcctc gcgggcttct
    25201 attcctatgg tgaaatcgca ccccacggcg cggacgcgga ctgcaccctg cacaatcaga
    25261 ccatgacgat cacccgcctc cacgaggagg cctgatgcct gacgtattcc atccgctgct
    25321 cctgcgtcag ctccggcgca cgtgcagcct gggcgatgcg gcggaacttg aaaccgccct
    25381 tgccgagctg cgcaaggcca gcctggatgg gcgcctggac ggcagcgctg cggccctggc
    25441 tcatgggctc gaacgcctgt tcgagcgggt gggcggcggc taccgtcagc tcgagcgcga
    25501 cctcgacctg cgcacccgca gcctcgaaca cagctctgcc gaactcaatg ccctcaacgc
    25561 ccggcttcag gatgatctgg aaagccgctc gcgcgccctc gccgcgctgc ggaccaccct
    25621 gatcgaactc caggacgcac cggcggcgcc ttcagcggac gcctcctccg aaggtctcga
    25681 agccacctcc cacctcctcg cccgcctcgt cgagagccgt cagcagaagg cacgggagct
    25741 ggactaccag aaatttgccc tcgaccagca cgccatcgtc gtggtcatga acgcgcacgg
    25801 catcatcgaa tacgcgaatg accgcttctg ccgggtttcc ggctaccagt ccgacgaact
    25861 ggtgggccgc caccggcaca tgctcaccag ccgcgatcac ccccgcagct tctttatcga
    25921 cttgtggcgc gccgtcaatg gcggcgacgt ctggcatggc cagatctgca accggcagcg
    25981 ggacggccgc ccctactggc tcgacaccac catcgtcccc ttccgcgacg gcgggggcgc
    26041 catctcccgt cacatcgcca tctgtaccga catcagcgcc atgaagcagc tcgaacaggc
    26101 gctgcgggag cagctcgcgc tgatcgagac gctcttcgag accatcccga taccgatcta
    26161 catcaaggac gcgggcgggc actaccaacg tttcaacaaa gccttcgagg aattcttcgg
    26221 catccgccgc gaggactggc tgggccggac cgcactcgaa gtgctacccg agtcgctggc
    26281 ccggcgccat gtcgctctgg acgaggaggt gctgggatgt ggcggcgtgc aggagttcga
    26341 gttcgaatgg cccttccccg gcggcacgcg ggtgggcgac taccgcaagg tggccctgcg
    26401 cgacccccac ggagccgtca ccggcctgat cggcgtgatc catgacatca ccccgcgcaa
    26461 gcaggctgag gaggccatgc gccaggcccg cgatgccgcc gaaaacgcca atcgggccaa
    26521 gagcgatttc ctggccaaca tgagccacga gatccgtacg cccatgaacg gcatcctcgg
    26581 catgaccgaa ctcaccctcg acaccgatct gaacgcggag cagcgggagt acctgcagct
    26641 cgtgcagacc tccgccgaat ccctgatggg catcatcaac gacatcctcg acttctccaa
    26701 gatcgaggct ggcaaggtgc tcgtcgagca catcgatttc gacctgcacc gcaccgtcac
    26761 cgaagccctc aagacgctcg cggtgcgcgc ctacgaaaaa aacctcgaac tgctctgcga
    26821 catcgatccg gcagtgcccc agcgcatctc cggcgatccc ggccggctgc gtcaggtact
    26881 gctcaacctg accggcaatg ccatcaaatt cacccctggc ggtgaagtcg ccgtcacggt
    26941 gcgctacgcc gcggacgccg gcgaagccgg tccgcgcatc catctggccg tacgggatac
    27001 gggcatcggc atcgcaccgg acaaacacac tgccatcttt gagcccttca gccaggaaga
    27061 cggctccacc acccgccgct tcggcggcac cggcctgggc ctcaccatct gccgccgcct
    27121 cgtggaagcc atgggcgggc ggctgtggct ggacagcaca cccggcacgg gcagcacctt
    27181 tcacttcacc ctcccggcgg ccaatccggc ggcagcgcct cgggaggccc cgccggcggt
    27241 actggccggc agacgggtcc tgatcgtcga cgaccacccg accaatcggc tcgtgcttga
    27301 acgcagcctg cggcgcatcg gcatgcaggc ctgcagcacc ggcgccagcg atcacgtcgt
    27361 ggccatcctg aacgccagcc agtgcgaggg cacccccaag gaggttgtcc tcatcgacag
    27421 ctgcatgccg ggcatggacg gcttcacgct ggctcaggcc atcgtcgccg aaggcttcga
    27481 tccgtgcccc gccctggtgc tgctgtcctc ggccggcgcc aagggcgatg ccgcccgctg
    27541 tcgcgagatc ggcatcgaag cctatctcac ccggccggtc agccaggacg aaatcagcca
    27601 gaccctgcag cgggtcatgg ccgcccgccc gcgccccgcg ccccggtcgc tggtcacccg
    27661 ccatgtgctc ggcgaggacg gcccccgcct caacctgctg gtgctggaag accaggccgt
    27721 gagtcgccgg ctgatgatcg ccctcctcac gcgcctcggg catcaggtcg agctggcaga
    27781 cacgctggcg ggcatcgccc ccgctgcgcg acgtttcggc ggaccggtgg atgtcgtgct
    27841 ggccagcctg caagtgccgg aactgccccc cagccgcgtg atcgccggcc tgcgggcggc
    27901 cggcatcgac gcgcccgtga ttggtgtaac cgcggatccg gacgaagacg tgcgcaacgc
    27961 ctgcgtcgcc gccggcatcg ccgacgtcct cgccaagccc ttgcagaccg gcgccctgcg
    28021 cgcagccctg gacgtcctcg cggcaacccg ctgcgatgtc cgggacgcgg cccgccatgc
    28081 gaccgcagcg ccccgtttcg actacgcccg cgccctgctg gacgccgacc gggagatcgt
    28141 ggacatgatc ggcgagcagt tgctggcgca gctgcctggc gagatcgccc agctgcgcag
    28201 cagcctgtcc ggcgcgccgc ccctcaccat ccaggccagg atccggaccc tgaagggtct
    28261 cctcgacacc gtgcgggccg aaccggccat ccgtctcctc gacgagctgg ccctacgcct
    28321 gagccagtcc gacaccccct acgcagacga cctgctggcc accctggacg ccgagatcgc
    28381 ggaatttacg cgagccctgc gcgcccgccg cccgggctga gggtccggcg cgccccgcga
    28441 acgcctccac agcgcgcccg catcggcccg gaggccggtg cgcggaccac cgaccggtct
    28501 cagcccaccc agcggcgcgc gttgcggaac atccgcagcc agggcgaatc ctcacccaga
    28561 ccggccggat gccagctcat ctgcaccgtg cgggcggtgc gttccgggtg cggcatcatg
    28621 atcgtgaagc ggccgtccgg cgtggtcagg ccagtgatgc cggccggcga accgttggga
    28681 ttaaagggat aacgctcggc caccgcgccg gtgttgtcca cgtagcgcag ggccacgcgg
    28741 gcgttgtcct gggcgtcgat gcccgcgaag acggcacgtc cttcgccgtg ggacaccacg
    28801 atgggcatgc ggctgccggc catgccggcg agcaggatgg acgggctgtc caccacctcc
    28861 accatcacga agcgggcctc gaactgctcg ctgcggttgc gctggaagcg cggccaacag
    28921 tcgccgccgg gaatgatctc ggccaggttg gacatcatct ggcagccatt gcagacgccc
    28981 agggcgaagg tgtcggtgcg ctggaagaag gcctcgaact ggtcgcgcag gcggctgttg
    29041 aagaggatgg acttggccca gccctgaccg gcgccgagga cgtcaccgta ggagaagcca
    29101 ccgcaggccg cgaggccctt gaagtcttcc agatggacgc gcccggcctg caggtcggac
    29161 atgtgcacgt cgtagggcgc aaagccggcc cgcgcgaagg cggccgccat ctcggcctgg
    29221 gaattgacgc cctgttcgcg caggatcgcc accttgggac gcgcgccggt ggcgatgaag
    29281 ggcgcgacga tgtcctcggc cggatcgaag ccgaggacca cgctcaggcc ggggttgccg
    29341 gcctcggcca gggcatcgaa ctcctcctgg gcgcaggtag cgtcgtcccg caggcgggcg
    29401 atctggtagc tggtctcgga ccagatccgg tgcagctcgg cccgcggtgc ggcgaactga
    29461 agcttggcgt tgcgccagaa gcgcagctcg tcccggtcat tgggctcacc gacgaagtgg
    29521 taattgaggc cggcctggcg caggggctcg gtgatgcggc tgcggtcttc gcggcggatc
    29581 tggatcacgg cgcccagttc ctcggcgaag agggccttga agagcatgtc gttgaagcgc
    29641 cccttgaggg cgtccgggcg cttgtcgagg ccgtccacgt cattcatgaa cgggtcgtag
    29701 gccaccgtgt cgagcatcac cgacaaaccg cagtgggacg cgaaggccat ttcacacagg
    29761 gtggcgaaga ggccgccgtc agagcggtcg tggtaggcca gcagcaggtc gtcccggcgc
    29821 cattgctgga tcaggtcgaa gaaggccttg agctgggccg gatccacgtc cggcgcgtgt
    29881 tccgccaccg agttgtaggc ctgggccagc accgagccgc cgaggcggtt gcggtggttg
    29941 cccagatcga tgagcagcag ctcggtctcg acgccttccg gaagctggag ctggggcgtc
    30001 agggtgcggc gcacgtcctg caccggcgcg aaggcggtga caatcaggga caagggcgag
    30061 acgacctgct tgtcctcacc gtcgtcgtgc caggcggtgc gcatggacag ggaatccttg
    30121 cccaccggaa tcgacagacc ggcgctctgg cagaagcggg agaccgcctc gacggtgcgg
    30181 aacagtttga cgtcctcacc gcgatggccg gcggcggcca tccagttggc ggagagcttc
    30241 acgtctccga ggctggcgat gtctgccgcg gcgaggttgg tgatcgcctc gccgatggcc
    30301 atgcgaccgg aggccggcgc gtccagcgtg gcgaccgggg tgcgctcgcc catggcgaag
    30361 gcctcgccgc gatagcccag gaagctcatg gtggtgacgg ccacgtcggc caccggcacc
    30421 tgccacgggc cgaccatctg gtcgcgggcg gtcatgccgc ccacggaacg gtcgccgatg
    30481 gtgatga
//
LOCUS       DAOQMM010000034        30418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_409_length_30418_cov_45.965254, whole genome shotgun sequence.
ACCESSION   DAOQMM010000034 DAOQMM010000000
VERSION     DAOQMM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 30418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30418
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_409_length_30418_cov_45.965254"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1333)
                     /locus_tag="PLH85_07770"
     CDS             complement(77..1333)
                     /locus_tag="PLH85_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNB64498.1"
                     /translation="MTTRRSFLKACAAAGALMPGLGIGRLAYAATDAAAPLTVVVFLR
                     GGCDGLLLAGPANEPAYVEARPPELRVLDGGERAGLPLANGLTADLDFRLHPDARPLY
                     ELYQAGRLALVHAVGLTNGTRSHFEAQDLIERGVSTLEQAYRTPEGWLTRYLTLYGGA
                     DGSADHLAALAAGAGLPASLARQGDALALPDLQGGVGLPGGTDGAALLGQLYAAGDSP
                     VHEAGRRALARLSQVDAHLPRTPDGKLAPYAPDGAGYESGAFSLGLQAIARVARMNVG
                     LRVACIDHASWDTHDAESGRFGGLVGQLSRGLAAFHRDLASAGIPFRLVAMSEFGRRL
                     RANRSQGTDHGHGGAMLVLGEGVRGGRMLGRWPGLSTPALDQGVDLAVTTDWRAVLAE
                     LLALDGRGRGLVFPGLPVEAPLGVLG"
     gene            complement(1338..2954)
                     /locus_tag="PLH85_07775"
     CDS             complement(1338..2954)
                     /locus_tag="PLH85_07775"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNB64499.1"
                     /translation="MSRRRIPATPEPARGPSPRQILNRLGFGIGPSDELARLARDGLP
                     AWLDAQLAPPPGDDGECLQRLGAARLRIRYDAGKAPGPEYPAVDENRPLETLQAPIET
                     LWPRTDYKRPMAGSERFRPRAEVAAATLIRAVHSRWSLREVLADFWHNHFNVDASQIV
                     QVAVALPAYDRDVIRAHALGNFREFLEAVATSTAMLYYLNNRSSRAGAANENYARELF
                     ELHTFGRGAYLNEFYNRWRDVPGALTGQPKGFIDQDVYEAARAFTGWTVEDGSGLGGS
                     DRLPATGRFAYVDSWHDNYQKRVLATEFDPFRPALEDGRKVLDLVAAHPATARFVSGK
                     LCRRLLGVEPSAGMLAAASETWRAALRAPDQIARVVRTIVLSPEFARSADLRVRRPLD
                     LVAALVRGAGMDFVPTEGLVNEMAAAGQRLFGWGAPTGHPEEVDYWLSSNGMRRRWLL
                     VQGLIENRWGNGVVEVRPPTPASTWREAADAWAEHLLGEAPGSTPVGQILAGLRQDGA
                     TPFDPGKPAQAAQMRRVLGYLAMGPGFQRG"
     gene            complement(2967..3299)
                     /locus_tag="PLH85_07780"
     CDS             complement(2967..3299)
                     /locus_tag="PLH85_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64500.1"
                     /translation="MKSLIPVSCLILLCGFAQAQASEAHTLRAPPADRADTCAHGGER
                     HGLEYAHWNEYLATRAGDSQSARDWAERARAECRELASERRDGPERPEARPDVRQERA
                     RRRELARR"
     gene            3613..7185
                     /locus_tag="PLH85_07785"
     CDS             3613..7185
                     /locus_tag="PLH85_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNB64501.1"
                     /translation="MAEQDPVRARPPGATLDDKYTAATGRVYLTGYQALVRLLLIQRD
                     RDRAAGLRTAGFVSGYRGSPLGGLDQTLWKAQAHLAGHDIVFQPGLNEDMAATAVWGS
                     QQVSLSPGARVEGVFAMWYGKGPGVDRCGDVFRHANAAGSARHGGVLVIAGDDHAAKS
                     STLPHQTDHFFKSMMMPVLAPAGVQEYLDFGVHGWAMSRYSGCWVAFKALADTVETSA
                     SVDVDPLRVVTRLPEDFALPPDGLNLRWPDPPLVQERRLLHYKLYAALAYCRANDLNR
                     VVIDSPEPRLGILTSGKSYLDVRQAFDDLGIDETLAARIGLRLYKVGMVWPLEAEGVR
                     RFAEGLDEILVIEEKRQLLEYQLKEELYNWREDVRPRVVGKFDEKGEWALLTTVGPDG
                     RASVDHGDWLLPAAGELTPAMIARVIAARIGRFFTSEHIQARLAFLDAKEHALAHRVF
                     ALDRVPTFCPGCPHNTSTRLPEGSRGLAGIGCHYMVSFMPDRRTGTFTHMGGEGVPWV
                     GQAPFTDERHIFANLGDGTYFHSGLLAIRQSVAAFNRPGATHGITYKILYNDAVAMTG
                     GQPVDGVLTVPALLRQLQAEGVHTIALVTDGTARPWHVADIPHGVTIHHRDELDVLQR
                     QLRSVPGVSALVFDQTCAAEKRRRRKRGTYPDPQTRVFINEAVCEGCGDCGRASNCMA
                     ILPVETEFGRKRRIDQSACNKDFSCMDGFCPSFVTVEGGALRRGSARHATDARFPALP
                     APVLPPTGRPWGILVTGVGGTGVVTIGALIGMAAHLDGKGVTVLDMTGLAQKGGSVFS
                     HIRIADRPEALHAVRIAAGEADAVIGGDVVVTASVDALAKMAVGRSHGVVNCAETPTS
                     DFTRNPDWQFPLERMQAALVEAVGADACDFLDATDLATRLLGDAIATNLFLLGYAWQK
                     GWVPVSEASLDRAIELNGAAVALNRAAFLWGRRSAVDRAAVEALARPAATPQDDTQTD
                     GLDARIARRAAFLTDYQDAAYARRYTDFVARVRTAEAPLGSERLGEAVADGYFRLLAC
                     KDEYEVARLFTAPAFRAAVDAAFEGDYRLRFHLAPTFLARPDPATGRLRKLAFGPWLM
                     TAFRGLAWLRRLRGTPFDLFAYHPERKAERALTAHYEADIADLLSDLSATTLHVAEAV
                     ARLPETIRGYGHVKADAMAQAAQRRTALLAELAALRALRRPSGT"
     gene            7266..7649
                     /locus_tag="PLH85_07790"
     CDS             7266..7649
                     /locus_tag="PLH85_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64502.1"
                     /translation="MLEPITDHKGLHIVSIGGRDAQARRYFVQDGEQLISKALPSLDT
                     ARAEAERLLAKRARGPNPWRAPWATHLIAPGVAVIQYPLGRALLLALSGGHYPPRGPH
                     NSLFVVSFPWMLLYAVFGVMSVFID"
     gene            7995..8804
                     /locus_tag="PLH85_07795"
     CDS             7995..8804
                     /locus_tag="PLH85_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNB64503.1"
                     /translation="MSYLAPTEFVTKMVDAGESKIFMSTRDTLIRAYMAGAILALAAA
                     FAVTINVQTGQPLAGAILFPVGFCMLYLLGFDLLTGVFTLAPLALLDKRPGVTVGGLL
                     RNWGLVFVGNFAGALTVAVMMAIIFTFGFTEAPNAIGQKIGTIGESRTVGYAAHGAAG
                     MLTLFIRGVLCNWMVSTGVVAAMMSTHVTGKIFAMWMPIMVFFYMGFEHSIVNMFLFP
                     SGLMLGGNFSLYDYMVWNEIPTVVGNLVGGITFVGLTLYSTHVRTAPKRNA"
     gene            8858..10588
                     /locus_tag="PLH85_07800"
     CDS             8858..10588
                     /locus_tag="PLH85_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB64504.1"
                     /translation="MPTSLTVTVGQYSDKGRKPANQDFHGLCIPVEPQLTTKGIAAAV
                     ADGISSSEVSHLASESAVKSFLGDYYCTAETWSVRTSAQRVLEATNAWLHAQTRNSPH
                     RYDKDRGYICTFSGLVLKSHTAHLFHVGDSRIARLRDGRLEVLTDAHRIVVSPEQSYL
                     ARALGFEAQLELDYRQVPIAAGDVFVLSTDGVHEHVGADVIVATLRAAPDLDAAARRI
                     ADAALAQGSADNLTIQLIRVDTLPVQAAAEHLQQFAELPLPPLLDARMEFDGYRILRT
                     LHGSSRSHLYLAEDIASGTRVALKLPSIDQREDKAYRERFMLEEWIARRLDSPHVLKP
                     CPVTRQRSYLYVATEYIEGSTLAQWMRDHPTPDVETVRGIVEQIARGLLAFHRLEMLH
                     QDLRPENIMIDTQGVVRIIDFGATHVNGLQETAANDTLAPILGTCQYTAPEYFLGEPG
                     TSRSDLFSLGVIAYQMLCSRLPYGTDVSQARTRSAQKKLRYRSVLSDTREIPAWLDAA
                     LAKAVHPDPLRRYAELSEFTYDLRHPNAALLGQRPPPLIERNPLLFWQILCALQTVLL
                     ILLLARRSLL"
     gene            10656..11453
                     /locus_tag="PLH85_07805"
     CDS             10656..11453
                     /locus_tag="PLH85_07805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB64505.1"
                     /translation="MRLPPALKALLAQLAGWAVAIGLLRAGLLPGQAWALVLAQAIAA
                     VSAAVALRSAPWWRWIHAGFTPLAALALQLGLHPAWYLAAFALLALVYWSSFRTQVPL
                     FLSNPATAAAVAQLVPPGHSARVLDLGSGTGALLRPLARLRPDCRLEGMESAPAPYLL
                     SRLLSRRHPNIALARGDFWQIPWTDYDLIYAFLSPVPMPEVWRKACAELRPGALLVSN
                     SFPVEGIEPDDIIHVADRRGTRLYCYHPAGPLPPDGRPVGSPVGLLP"
     gene            11568..12266
                     /locus_tag="PLH85_07810"
     CDS             11568..12266
                     /locus_tag="PLH85_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64506.1"
                     /translation="MSTIICIIGNKGGTGKTTLSHMLCQGFGLLGQRSACVLTDVSRE
                     PLSPDGRRYVTADGRSPEALHKVVGKLRTMQDWIGVIDGGGNRSEVDRLLDSYADIVL
                     LPFRDSHEDMRTVRRDLETFPRAYGLPSQWATNAWQRDAADRAVLELLGDHRDRLLHP
                     VASLSASKLLLQKHIPASLPSNLGNACRALARQVMDVLETDADALGTDAHEAAHSLPA
                     PTVLPAGYASTAHH"
     gene            12454..13068
                     /gene="ccmA"
                     /locus_tag="PLH85_07815"
     CDS             12454..13068
                     /gene="ccmA"
                     /locus_tag="PLH85_07815"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNB64507.1"
                     /translation="MLRAHGLACDRGDRRLFSGVDCAVAPGEWLQVEGPNGAGKTSLL
                     RILAGLAPAAAGHIEWEGRPVADVRDEYHARLLYLGHAPSIKDDLSALENLTIAAAVA
                     GRPLDEAAALGALRRIGLKGREDLPSRVLSQGQKRRVALARLLCSQARLWILDEPFVA
                     LDVAAVDQLCAILDDHLGRGGMLLFTSHQAVQLSGAGGSLRLGA"
     gene            13065..13730
                     /gene="ccmB"
                     /locus_tag="PLH85_07820"
     CDS             13065..13730
                     /gene="ccmB"
                     /locus_tag="PLH85_07820"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNB64508.1"
                     /translation="MNAFIAIVRRDLLLAARRKSEVLTALFFFLIVVSLFPLGIGPET
                     AMLRRIAPGVLWVAALLAAMLSLHRLFAADHQDGTLEQMALSPVPLALLVGGKIVAHW
                     LTSGLPLVLLAPVLGIQFDLAPQALGILMLSLLLGTPVLSLIGAIGAALTLGVRGGGV
                     LLSLLVLPLYIPALIFGAGAVEAEMAGLGAGGHLSLLGAVLALGAFFAPWAATAALRI
                     ALE"
     gene            13752..14501
                     /gene="ccmC"
                     /locus_tag="PLH85_07825"
     CDS             13752..14501
                     /gene="ccmC"
                     /locus_tag="PLH85_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HNB64509.1"
                     /translation="MSQRLINWFKFASPASFYPLAGRAWPWFAALAAAFAAAGLWLGF
                     FVAPTDAQQGEGYRIIFIHVAASWMSMFIYCVMAFWSALGISLNTRLSGMMCSALAPT
                     GALFAFLSLWTGALWGKPMWGAWWVWDARLTSELILFFLYLGYIALTSAIDDPRRADK
                     AGAIISLVGVINVPIIYFSVKWWNTLHQGASVSLTKAPSMATTMLWGMLLMALAFWMY
                     SIATALYRVRAIILERERHAAWVAELPEMQA"
     gene            14498..14686
                     /gene="ccmD"
                     /locus_tag="PLH85_07830"
     CDS             14498..14686
                     /gene="ccmD"
                     /locus_tag="PLH85_07830"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNB64510.1"
                     /translation="MNWGSASEFFAMGGYGLYVWGSFGVTFGALLIEALLTRARFRAA
                     RRALRRELAADRESQDHP"
     gene            14683..15123
                     /gene="ccmE"
                     /locus_tag="PLH85_07835"
     CDS             14683..15123
                     /gene="ccmE"
                     /locus_tag="PLH85_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNB64511.1"
                     /translation="MKARHKRFAIVLGGLAALGIAATLVLNAFQSNLVFFFTPTQVAA
                     GEAPKDRAFRIGGLVKEGSLQRTDITARFTVTDTAKEIPVTYTGILPDLFSEGKGVVA
                     QGRLGVDGQFTASEVLAKHDENYMPPEAKAAVDQAHQAAKSLKQ"
     gene            15140..17095
                     /locus_tag="PLH85_07840"
     CDS             15140..17095
                     /locus_tag="PLH85_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNB64512.1"
                     /translation="MIPELGSFALVLALLVALVQGVLPLVGAQRGIPAWIALARPASA
                     TLFLLVAVAFSCLTASFINNDFSVLYVVQHSNSRLPVEYRIAGVWGGHEGSLLLWVQM
                     LALWSLAVAAFSRSLPEDMVARVQAVLGLVAAGFILFILTTSSPFERLLPGAEDGRDL
                     NPLLQDPGLIFHPPMLYMGYVGFSVAFAFALAALLSGRLDAAWARWSRPWTTAAWIFL
                     TLGIGLGSIWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGSFKNWTV
                     LLAIGAFSLSLLGTFLVRSGVLSSVHAFAADPKRGIFILAFLAVVIGASLTLFAWRAP
                     KVGLGGAFALVSRETLLLVNNVLLLTAAGAVLLGTLYPLIIDALNLGKLSVGPPYFNT
                     VFVPLMVPVLFLMAAGPVARWKQADLGEIARRLRWAFGVAVAAGVSLPFVSGGWSAGV
                     ALGLALALWIFGSAIVQIRERLKTGRPPRAFWGMHLAHVGVAVTVLGIVMVKNYESER
                     DVRMAPGDTVKAGDYVLRFKGVESVPGPNYTAARGDLDLIRDGQVVLQLQPEKRKYLS
                     SAMPMTEAAIDSGLTRDVYVSLGDPLDGEDGAWSVRVYYKPFVAWIWAGALLMALGGL
                     LAASDRRYRLTAKAARATALAGQGAAS"
     gene            17092..17682
                     /locus_tag="PLH85_07845"
     CDS             17092..17682
                     /locus_tag="PLH85_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNB64513.1"
                     /translation="MKRFLIPLALFLVLVGFLAIGLNRDPHEVPSPLVGKPAPDFRLE
                     VLAEPGRTFTPADMRGKVWLLNVWASWCVSCRAEHPLLVAFSKQGGVPIVGLNYKEVR
                     GDGQTDVKGLAPADEQALTRQRAAEWLARHGNPYSLSVLDMDGRVGIDYGVYGVPETY
                     LIDKKGVIRYKQIGPITPEALEQKILPLARQLEQEA"
     gene            17684..18235
                     /locus_tag="PLH85_07850"
     CDS             17684..18235
                     /locus_tag="PLH85_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNB64514.1"
                     /translation="MTMPSRAQRVRPAITMRGRLLATAAALLVAGSPVLAADARPLAD
                     DPIVEARMLTISEELRCLVCQNETLAASRAELAEDLRREVRSLIRQGKSDTEIKDYLV
                     TRYGDFVLYRPPVKSTTWLLWFGPFLLLAGGVAGLVAYLRRRAGRVAPPTLSAADRAA
                     AEALLAGSADAPAGSTSSPKDQA"
     gene            18232..19455
                     /gene="ccmI"
                     /locus_tag="PLH85_07855"
     CDS             18232..19455
                     /gene="ccmI"
                     /locus_tag="PLH85_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNB64515.1"
                     /translation="MTAFWIAAGLLTALVLALLCRPLLHRRDATGASRKALNAAIYRD
                     QMAELERDLQSGVLSRDDYAVARDELERRVLEDVAADDTPTDTRRRLPRSAVGLAVLL
                     PAAAVGLYFVLGTPAALDPARVAGTEQAPSQADIEKMVAALAAKLDKEPNNPQGWVML
                     GRSYRVMGRLDEAEKAFGRAGPVMESEPALMLELAELSADRNEGRVEGHARELLLRVL
                     KDAPENPQALVIAGTDAFFRKDFAAAIRHWEKVLALVPPDSEDATNLTAGIERARAQL
                     GQGTAAARPATATPVKDAAASAVKGRVELAPGVHAAPDDTVFIFARAFDGPRMPLAVI
                     RTKASALPMDFTLDDSLAMSPDLRLSGAAQLRIEARVSRSGDALARPGDFTASVGPVK
                     PGTRGLRLVIADPVK"
     gene            complement(19480..21909)
                     /locus_tag="PLH85_07860"
     CDS             complement(19480..21909)
                     /locus_tag="PLH85_07860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB64516.1"
                     /translation="MFAQFRRSLRARIAAGVLGGVFVTLWLTYVTLDYFLRREMEATL
                     SAQQFSVVSLLADDIDRSVRERLQALEAVAEVLQQAGRGASGTPSDVLRQQPALLSLF
                     NWGVLVTDREGTALVSLPESMGRQGVNYGEVESIRLAVSEGRAQIGRAVFGKTTGKPV
                     IPMVVPVRDRDGRISGTLIGLTNLAAANFLDDISSNRFGRRGGYLITDPEQRIFIAAT
                     DKTRVMQAGPPVGVNPVFDRYLEGYDGSGVARSSRGVVELSSSKRVPSTGWLMQAVLP
                     SEEAFAPIDEVLQRIVVASVAVGLLAAGLVLWWLRHELRPLGEAASMLGQMRDGLIPR
                     QPLPVRRDDETGALAGAFNGLLAAIVAEEARAAEHLFNQRLRAIVSRVPGIVIQYQRY
                     DDGRYALPFASDALREICGLEASAVEHSADDFHALLHADDAEAFFASRDRSAEALEPW
                     HCEFRIRHPEAGVRWLLLDAMPEPDAAGLSWHGFVTDITGLKEIESELRIAAATFETQ
                     EGIFITDAEKRIVRVNQAFTAMTGYSVEEAVGQRPSLLRSGLHSADFYRHLHESLAKD
                     GFWQGEIWNRRKNGEIYAEWVTISAVQDTAGRVTNYVAAFSDITEHKKSEEQIHRLAF
                     YDPLTQLPNRRLFYDRLEQAAVASARAGYGCALLFLDMDGFKSLNDRYGHALGDELLV
                     TVAQRLSVCVRESDTVARLGGDEFVVILEHLGDEPAAAAQAEMVAQKIREALVAPYEL
                     RGRNGDTHVTFNCSASIGVSLFRGHELSREDIIKRADIAMYEAKAGGRNTIRFYTPAC
                     EVAAGLKAAAG"
     gene            22155..22532
                     /locus_tag="PLH85_07865"
     CDS             22155..22532
                     /locus_tag="PLH85_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB64517.1"
                     /translation="MTSSTPPPDPGRPPTVRNLFADRPSAGPDEHFDTLLNHPQARIE
                     RILSHRHASPPDFWYEQPGDEWVLLVHGSATLEFDNGQRTELRPGDWLTLPAGCRHRV
                     AATGPDTVWLAVHLSRGSVSLSG"
     gene            22555..23550
                     /locus_tag="PLH85_07870"
     CDS             22555..23550
                     /locus_tag="PLH85_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB64518.1"
                     /translation="MIRRRIALLTFSALACTAAVAPVGPLVGAAHAEAPAVRTQVPGF
                     YRMALGDAEVTALYDGYIDLDTKILSNASPAEIQRLLARMFIAGEKVQTAVNTYLVNV
                     GGKLILIDTGTAKAFGPTLGFVADHLRAAGYTPEQVDVVLLTHLHPDHVNGLLGADGK
                     PLFPNAQVRVAQVESDFWLSADNTAKAPKDFQPFFKMAQAAAAPYQASGQWKPFHDES
                     EVAPGIKPVPAVGHTPGHSAYVVESKGERLLILGDTVHSYAVQFARPEVAIEFDTDKK
                     QAVATRKKLFAWAAREKLAVAGMHLPFPGLGHIRSEGKAFAWVPVEYSPLRDRAK"
     gene            complement(23679..24815)
                     /locus_tag="PLH85_07875"
     CDS             complement(23679..24815)
                     /locus_tag="PLH85_07875"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNB64519.1"
                     /translation="MIDDLCLLDDALDGAAECLQRWRASRPAAGVLALVAEASRDRVP
                     ALQAIARAAAMPLVGAVFPALVTATGFAVRGTWLLCLDPMPPTFLLEDVATADGVERL
                     AAAMGAHLQSPDGRAPPLMFTIFDALVPNIGTLMMTAHSRLPAPPRHAGANAGSERFQ
                     PIPCLFDATREVAGGVLGLCLPPDMAAVVRHGYPVSGTQMRATSTTGNRIDRIDGRPA
                     FTVYREVIWASFGVALTHANFYEYAVHFPLGVITMFDVLVRIPVGFTDDGAVVCVGEV
                     PPNSVLRVLEAPAAADDALLDGIVRGLNVAPGTMQTPLLTFYCAGRRMHFGAAESGRE
                     VERLRGRAGAAQIVGALSLGEIDSIEDLGFPRFHNAALVCLART"
     gene            complement(24922..26088)
                     /gene="metK"
                     /locus_tag="PLH85_07880"
     CDS             complement(24922..26088)
                     /gene="metK"
                     /locus_tag="PLH85_07880"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNB64520.1"
                     /translation="MAKEYLFTSESVSEGHPDKVADQISDAVLDAILAQDPRGRVACE
                     TLVSTGLVVISGEITTRADINYMDIARETVKRIGYDDSEIGFDYKSCAVLTAINRQSS
                     DIAQGVNEGEGLDLDQGAGDQGLMFGYACNETPSLMPFPIYYAHRIMQRQGEVRKDGR
                     LPWLRPDAKSQLTVKYVDGKPVAIDTVVVSTQHHPDVSHAALSEAVIEEVIKPVLPKE
                     MLTPATRFLINPTGRFVIGGPNGDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDR
                     SAAYAGRYVAKNIVAAGLAERCEVQVAYAIGVARPVSLMVDTFGTGRISDEKIVELVE
                     AHFDLRPKGIIASLDLLRPIYSRTAAYGHFGRDEVDFTWETTDKAAALREAAGL"
     gene            26316..27191
                     /locus_tag="PLH85_07885"
     CDS             26316..27191
                     /locus_tag="PLH85_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB64521.1"
                     /translation="MASLFFSLLARLPLGLLQQLGAGLGWLTYALSGSYRRRLRENLE
                     RALGPEAAARVRPAAIADAGRQALELPWILLRPQADLARKMVAVSGWEHVEAAEARGG
                     GVLFITPHLGCFEITAQYFSLHKPITVLYRPPKKALLQPLIDAGRSRGNMHLAPADTA
                     GVRRLVKALRAREAVGMLPDQVPGAGEGQWVPFFGRPAYTMTLAARLSEVAGVSTLFA
                     WAERLPGGRGFHLHLRPALIPLTGDTAARCAAINREMEAQIRACPAQYLWGYNRYKRP
                     AGAPAAPPTAEVPPA"
     gene            27188..28060
                     /locus_tag="PLH85_07890"
     CDS             27188..28060
                     /locus_tag="PLH85_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HNB64522.1"
                     /translation="MSRLLVALLWLIHWLPLSVQAPIGRGLGHLLYWLIAPRRKVALT
                     NLRLCFPELSEAERVALCKEHFALAGRSLLERGIAWFASEERVRRIVRLDNEPMLRRL
                     IEGGQPVILLAPHFLGLDLGGSRMAIAFDSVSIYARQSDPVIDRWLYHGRSRFGNQLL
                     LARDESIRASIKAMKALRPFYYLPDMDHGAEESVFVPFFGVQAATLAALPRFAKLGGA
                     VVVPCVTRMLPGGLGYVVELGEPWQDFPGPSVEGDARRMNAFLEDRIRSMPAQYYWVH
                     RRFKTRPPGEPPIY"
     gene            complement(28088..28318)
                     /locus_tag="PLH85_07895"
     CDS             complement(28088..28318)
                     /locus_tag="PLH85_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64523.1"
                     /translation="MGLKRLPAGAWLMGVPGLACLIAGALLLAGFGAELHPLLQESGA
                     GLVLIVSGVALVGSAAFPLVLAELAAKDDPTE"
     gene            complement(28321..29061)
                     /gene="trmB"
                     /locus_tag="PLH85_07900"
     CDS             complement(28321..29061)
                     /gene="trmB"
                     /locus_tag="PLH85_07900"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNB64524.1"
                     /translation="MNDTPHIPADAQPADAPEGASAPRLTSQSIRSFVLRQGRMSEAQ
                     HRFLDEMMPRVGLDYRPAPVDLDAVFGRTAPHIVEIGFGMGASTAEIARARPDEDFVG
                     IEVHAPGVGSLCKLIDEGGLHNLRIVQHDAVEVLRDMIPEASLAGVHIYFPDPWPKKR
                     HHKRRLVQGPFVRLIASRLAPGGYLHCATDWEDYAVQMQDVLSAEPLLANTADGYAPR
                     PDYRPLTKFEQRGLRLGHGVWDLIFRRV"
     gene            complement(29137..29922)
                     /locus_tag="PLH85_07905"
     CDS             complement(29137..29922)
                     /locus_tag="PLH85_07905"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNB64525.1"
                     /translation="MDDALTIAGKRYSSRLLVGTGKYRDFAQTREAVLASGAQIVTVA
                     IRRTNIGQDPNAPSLLDALPPSEFTYLPNTAGCYSAEDAVRTLRLARELLDDHRLVKL
                     EVLGDPHTLYPNMPETLQAARTLVKEGFEVMVYCSDDPIQAKMLEDIGCVAVMPLASL
                     IGSGMGVLNPWNVQLVRDAVKLPLLLDAGVGTASDAAIAMELGCDGVLMNTAIALAQD
                     PVRMAAAMKKGVEAGREAFLAGRMPRKFYTASPSSPTTGLIGS"
     gene            complement(30002..30238)
                     /gene="thiS"
                     /locus_tag="PLH85_07910"
     CDS             complement(30002..30238)
                     /gene="thiS"
                     /locus_tag="PLH85_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNB64526.1"
                     /translation="MFTDSRPPGGAVLSLMVNGEARVLPAPATVFELLTSMGLVGKRV
                     AVERNGDIVPKGLHAGTALADGDRLEIVVAVGGG"
     gene            30308..30381
                     /locus_tag="PLH85_07915"
     tRNA            30308..30381
                     /locus_tag="PLH85_07915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30340..30342,aa:Gly,seq:ccc)
BASE COUNT         4553 a        11647 c         9597 g         4621 t
ORIGIN      
        1 aggcggtcca gcagaccgcc gtgcatgcgg gcgaggtgga tctgttctga cgccggccgg
       61 gcaccggagc aaagcctcag cccagcacac caagaggtgc ttcgaccggc aggcccggaa
      121 acaccagccc gcgtccgcgc ccgtcgaggg ccaacagctc ggccagcact gcacgccaat
      181 cggtggtgac ggccagatcc accccttgat cgagggccgg cgtactcagc cccggccaac
      241 gccccagcat gcggccgccg cgcacgccct cgcccagcac cagcatcgcg ccgccatgcc
      301 cgtggtccgt gccctggctg cggttggccc gcagacgccg cccgaactcg ctcatggcca
      361 ccagccggaa gggaatcccc gcgctggcta ggtcccggtg aaatgcagcc agcccccgcg
      421 acaactggcc gaccagcccg ccgaagcgcc cgctttccgc gtcgtgggtg tcccaactgg
      481 cgtggtcgat acacgcgacc ctcaggccga cgttcatccg cgccacccgg gcaatggcct
      541 gcaggcccag gctgaatgcg ccggactcgt agcctgcgcc gtcgggcgca tatggcgcga
      601 gcttgccgtc cggcgtgcgc ggcagatgcg catccacctg ggacaggcgc gccagcgccc
      661 ggcgcccggc ctcatgcacc ggcgaatcgc ccgcggcgta gagttgcccg agcagcgccg
      721 cgccgtccgt gccaccgggc agtccgacgc cgccctgcag atcaggcagg gcgagggcgt
      781 cgccctggcg ggccagggag gccggcagac ccgcgcctgc cgccagggcc gcgaggtggt
      841 cggcactgcc gtccgcgccg ccgtacaggg tcaggtagcg ggtcagccag ccttcgggcg
      901 tgcgatacgc ctgttccagc gtgctcaccc cgcgctcgat gagatcctgg gcctcgaagt
      961 ggctgcgcgt cccgttggtc aggccgaccg catggaccag cgccaggcgc cccgcctgat
     1021 agagctcgta cagcgggcgc gcgtccggat ggagccggaa atccagatcc gcggtcagcc
     1081 cgttggccaa gggcaggccc gcccgctcgc cgccatccag cacccgcagc tccgggggac
     1141 gcgcctccac gtaggccggc tcgttggcgg gcccggccaa cagtagcccg tcacaccccc
     1201 cgcgcaggaa gaccaccacc gtcaacggcg ccgccgcgtc ggtcgctgca taggccaggc
     1261 gcccgatgcc caggccgggc atcagggcgc ccgccgccgc acaggctttg agaaagctgc
     1321 gccgtgtcgt catgtcctca tccccgctgg aagcccggcc ccatggccag gtaacccagt
     1381 acccgccgca tctgagctgc ctgggccggt ttgcccggat cgaagggcgt agccccgtcc
     1441 tggcgcaggc cggccaggat ctgcccgacg ggggtactcc ccggcgcctc acccagcagg
     1501 tgctcggccc aggcatcggc cgcctcgcgc caggtggacg ccggggtggg tgggcgaacc
     1561 tccaccacac cgttgcccca gcggttctcg atgagtccct gcaccagcag ccagcggcgc
     1621 cgcatgccgt tcgacgacaa ccagtaatca acctcctccg ggtggccggt tggcgcgccc
     1681 caaccaaaca gacgctgccc cgcggccgcc atctcgttga ccagcccttc ggtaggcacg
     1741 aagtccatgc ccgccccccg caccagcgcc gccaccagat ccagtggccg ccgcacccgc
     1801 aggtccgccg aacgggcaaa ctccgggctg agcacgatgg tgcgcaccac ccgggcgatc
     1861 tggtccggcg cccgcagcgc cgcgcgccac gtctcggacg ccgccgcgag cattccggcc
     1921 gagggctcga cacccagcaa gcggcggcac agcttgccgc tcacgaaacg cgccgtggcc
     1981 ggatgggccg ccaccaggtc gagcaccttg cgcccatcct ccagcgccgg ccgaaagggg
     2041 tcgaactcgg tggccagcac gcgcttttgg tagttgtcgt gccagctgtc tacgtaggcg
     2101 aagcggccgg tggccggcag gcggtcgctg ccgcccaggc cgctgccgtc ctccaccgtc
     2161 cagccggtga aggcgcgggc ggcctcatag acgtcctggt cgatgaagcc cttgggctgc
     2221 cccgtgagag cgccgggcac atcgcgccag cggttgtaga actcgttcag gtacgcgccg
     2281 cgcccgaagg tgtgcagctc gaacagctcc cgcgcgtagt tctcgttggc ggcgccagca
     2341 cgcgaggaac gattattgag gtaatacagc atcgccgtgc tggtggcgac ggcctcaagg
     2401 aattcccgga agttgcccag ggcgtgggcg cggatcacgt cccggtcgta ggcgggcagg
     2461 gccaccgcca cctgcacgat ctgggaggca tctacattga agtggttgtg ccagaaatcc
     2521 gccagcactt cgcgcagcga ccagcgactg tggacggcgc ggatcagggt tgcagccgcg
     2581 acctcggccc gcggccggaa acgctccgac ccggccatcg ggcgcttgta atcagtgcgc
     2641 ggccacaggg tctcgatggg cgcctgcagg gtctccagcg gccggttctc gtccacggcc
     2701 gggtattccg gtcccggcgc cttgcccgcg tcgtagcgga tgcgcagtcg tgcggcgccc
     2761 aggcgctgca ggcattcgcc gtcgtcaccg ggcggcggcg ccagttgcgc gtcgagccac
     2821 gccggcaggc cgtcgcgggc cagccgggcg agctcgtcgg acggaccgat gccgaatccc
     2881 agccgattca ggatctggcg gggagacggt ccgcgggccg gctcgggcgt cgcgggaatg
     2941 cgccgacgtg acatggccga agcgggtcag cgccgcgcca gttcccgccg ccgggcacgt
     3001 tcctggcgca cgtccggccg cgcttcgggc cgctcggggc cgtcgcgacg ctcactcgcc
     3061 agctcccggc actcggcgcg ggcgcgctcg gcccagtcgc gggcgctctg gctgtcgccg
     3121 gcgcgggtgg ccaggtattc gttccagtgg gcgtattcca gcccgtgacg ctctccgccg
     3181 tgggcgcacg tgtccgcccg atcggcgggc ggagcgcgaa gcgtgtgggc ttcgctcgcc
     3241 tgggcctggg caaagccgca cagcaggatg aggcaggaga cggggatcag ggatttcatg
     3301 gtgtatgtcc agggtcgggc agcgcagacg cgggccgcag cggcaattca gcccattaac
     3361 gcccggaagg ccggaaagga gaagtcttgc ggtcgaaaaa atcttttctg tttgtaagca
     3421 tcatgcagac ggcctgccgg cacatcggtg aaaaccgcag ccgtcgtgca acctgtggca
     3481 cagcctgcgt tccaaggttt gagcggcagg gccttccaga ctacgctgca agtgcgacac
     3541 ctccatggca aaggccacaa gcctaacgcc ccggacgcag cccgaacatg attttgcgca
     3601 aggaggttca ccatggccga acaggatccc gtccgcgccc gcccgcccgg cgccacactc
     3661 gacgacaaat acaccgccgc caccggccgc gtgtatctga ccggctacca ggccctcgtg
     3721 cgcctcctgc tgatccagcg tgaccgcgac cgcgccgccg gcctcaggac cgccggcttc
     3781 gtctcgggct atcgtggctc cccgctcggc gggctcgacc agaccctgtg gaaggcccag
     3841 gcccacctcg ccggtcacga catcgtcttc cagcccggcc tcaatgagga catggccgcc
     3901 accgcggtgt ggggcagcca gcaggtgagc ctctcccccg gcgcgcgcgt ggaaggcgtt
     3961 ttcgccatgt ggtacggcaa ggggccgggc gtggaccgct gcggcgacgt gttccgccac
     4021 gccaacgcgg ccggcagcgc ccgccacggc ggtgtgctgg tgatcgccgg cgacgaccac
     4081 gcggccaagt cctccacgct gccgcaccag accgaccact tcttcaaatc catgatgatg
     4141 ccggtgctgg cgccggcagg cgtccaggaa tacctggatt tcggcgtgca tggctgggcc
     4201 atgtcccgct attccggctg ctgggtggct ttcaaggctc tcgccgacac ggtggagacc
     4261 tcagcctcgg tggacgtgga tccgctgcgc gtcgtcaccc gtctgcccga ggacttcgcc
     4321 ctgccgccgg acggcctcaa cctgcgctgg cccgatccgc ccctggtaca ggaaaggcgc
     4381 ctgctccact acaagctcta cgcggccctc gcctactgcc gcgccaacga cctcaaccgg
     4441 gtggtcatcg actcgcccga accgcgcctg ggcatcctca ccagcggcaa gagctacctg
     4501 gacgtgcgcc aggccttcga cgacctgggc atcgacgaga ccctcgccgc gcgcatcggc
     4561 ctgcgcctct acaaggtggg catggtatgg ccgctggaag ccgagggcgt gcgccgcttc
     4621 gcagaaggtc tggacgagat cctggtcatc gaggagaaac gccagctcct cgaataccag
     4681 ctcaaggaag agctctacaa ctggcgcgag gacgtgcgcc cgcgggtcgt cggcaaattc
     4741 gacgagaagg gcgaatgggc cctcctcacc accgtcgggc cggacggccg cgcgtcggtg
     4801 gaccacggcg actggctgct gccggcggcg ggcgaactca ccccggcgat gatcgcccgc
     4861 gtcatcgccg cgcgcatcgg gcgcttcttc acctcggaac acatccaggc acgcctcgcc
     4921 ttcctcgacg ccaaggaaca cgccctcgcc caccgggtct tcgccctcga ccgggtgccc
     4981 accttctgtc ccggctgccc ccacaacacc tccacccgcc tgcccgaagg cagccgcggc
     5041 ctggccggca tcggctgcca ctacatggtc agcttcatgc ccgaccgccg caccggcacc
     5101 ttcacccaca tgggcggcga aggcgtgccc tgggtcggcc aggccccctt cacggacgaa
     5161 cgtcacattt tcgccaacct gggtgacggc acttatttcc actcgggcct gctggccatc
     5221 cgccagtccg tggccgcctt caaccgccct ggcgcgaccc acggcatcac ctacaagatc
     5281 ctctacaacg acgccgtcgc catgaccggc ggccagcccg tagacggcgt cctcaccgtc
     5341 cccgccctcc tgcgccagct ccaggccgaa ggcgtgcaca cgatcgccct ggtcaccgac
     5401 ggcaccgcgc gtccgtggca cgtggcggac atcccccacg gcgtcacgat ccatcaccgg
     5461 gacgagctgg acgtgctgca gcgccagctg cgcagtgtgc ccggcgtctc cgcgctggtc
     5521 tttgaccaga cctgcgccgc cgagaagcgc cgccgccgca aacgcggcac ctaccccgac
     5581 ccgcagaccc gcgtcttcat caacgaagcc gtctgcgaag gctgcggcga ctgcggccgc
     5641 gcctccaact gcatggcgat cctgccggtg gaaaccgaat tcgggcgcaa gcgccgcatc
     5701 gaccagtccg cctgcaacaa ggatttctcg tgcatggatg gtttctgccc gagcttcgtc
     5761 accgtggaag gcggcgccct ccgccgcggc agcgcccggc acgccaccga cgcccgcttc
     5821 ccggcgctgc cggcccccgt cctgccgccc accgggcggc cctggggcat cctcgtcacc
     5881 ggcgtggggg gcaccggcgt ggtcaccatc ggcgccctca tcggcatggc cgcccacctg
     5941 gacggcaagg gcgtcaccgt gctcgacatg accggcctcg cccagaaggg cggctcggtc
     6001 ttcagtcaca tccgcatcgc cgaccggccc gaggcgctgc acgccgtgcg catcgccgcc
     6061 ggcgaggccg acgccgtgat cggcggcgac gtggtggtca ccgccagcgt ggacgccctc
     6121 gccaagatgg ccgtcggacg cagccacggc gtcgtcaatt gcgccgagac gcccacctcc
     6181 gacttcaccc gcaacccgga ctggcagttc cccctcgagc gcatgcaggc cgccctcgtc
     6241 gaagcggtgg gcgccgacgc ctgcgacttc ctcgacgcca ccgacctggc cacgcgcctg
     6301 ctcggcgacg ccatcgccac caacctgttc cttctcggct acgcctggca gaagggctgg
     6361 gtcccggtca gcgaggccag cctcgaccgg gccatcgaac tcaacggcgc cgccgtcgcc
     6421 ctgaaccgcg ccgccttcct gtggggccgc cggagtgccg tggatcgggc cgccgtcgaa
     6481 gccctcgccc gcccggcggc gacaccgcag gacgacaccc aaaccgacgg cctcgacgcc
     6541 cgcattgccc gccgcgccgc cttccttacc gactaccagg acgccgccta cgcccgccgc
     6601 tatacggatt tcgtcgcccg cgtgcggacc gccgaagcgc ccctcggcag cgaacgcctg
     6661 ggcgaggccg tcgccgacgg ctatttccgg ctgctcgcct gcaaggacga gtacgaagtc
     6721 gcccggctgt ttaccgcgcc cgccttccgc gccgccgtgg acgccgcctt cgagggcgac
     6781 taccggctgc gcttccatct cgcccccacc ttcctcgccc gccccgaccc ggccaccgga
     6841 cgcctccgca agctcgcctt cggcccctgg ctgatgacgg ccttccgcgg cctggcctgg
     6901 ctgcgacgcc tgcgcggcac gcccttcgac ctcttcgcct accacccgga acgcaaggcc
     6961 gaacgcgccc tcaccgcgca ctacgaggcc gacatcgccg acctgctgag cgacctcagc
     7021 gccaccaccc tccacgtcgc cgaagccgtc gcccgcctgc ccgagacgat ccgcggctat
     7081 ggccacgtga aggccgacgc catggcccag gcggcgcaac gccggaccgc gctgctcgca
     7141 gaactcgccg ccctgcgcgc gctgcgccgc ccctccggca cctgaaccgc cagccggaga
     7201 acatggcgac cggcggcgcc cgcgcctcga ctatactcat gccgcccgca tcgaccggaa
     7261 ccgccatgct cgaaccgatc accgaccaca agggcttgca catcgtgtcc atcggcggcc
     7321 gcgatgcgca ggcgcgccgc tatttcgtcc aggacggcga gcagctgatc tccaaggcgc
     7381 tcccgagcct ggacacggcg cgggccgaag ccgagcgcct gctcgcgaag cgcgcccgcg
     7441 gccccaatcc gtggcgcgcg ccctgggcca cccacctcat cgcaccgggc gtcgccgtga
     7501 tccagtatcc cctcggccgg gcattgctgc tggccctcag cggcggacac tatccgcccc
     7561 gcgggcccca caacagcctc ttcgtcgtga gctttccgtg gatgctcctg tacgccgtgt
     7621 tcggcgtgat gtcggtcttc atcgactgag gccgcgcggg tcgacgcacc tttccgcggc
     7681 gcacgcccag gcgcctgaca cgctacgcac caataacgtg cctgatccgc agcaccgcac
     7741 ccaatcgggg cgcattctgc catccatgcc ctgcgaaaac ccgcagcgcc gccctcattt
     7801 gccttggcgt gaaacctgct ttatcaccct ccagagctga accaacgctg gttcagtgtt
     7861 cctttgtttg atggataacg gcgtccattt ccgtcccgca ccgcgggagg aaacggacgc
     7921 cgtttttcgt ttccgcattg cctgtcgatg cggaaacgcc attccggaat cttcacgacc
     7981 gcaggagacg caccatgtcc tatctcgccc cgaccgaatt cgtcaccaag atggtggacg
     8041 ctggggaatc gaaaatcttc atgtccaccc gcgacaccct gatccgcgcc tacatggccg
     8101 gcgccatcct ggccctggcg gcggccttcg cggtcaccat caatgtccag accggccagc
     8161 cgctggccgg cgccatcctc tttccggtgg gcttctgcat gctctatctg ctgggcttcg
     8221 acctgctcac cggggtgttc acgctggcgc ccctggccct gctggacaag cgtcccggcg
     8281 tcaccgtcgg cggcctgctg cgcaactggg ggctggtctt cgtgggcaac ttcgccggcg
     8341 ccctgacggt ggcggtgatg atggccatca tcttcacctt cggcttcacc gaggcgccca
     8401 acgccatcgg gcagaagatc ggcaccatcg gcgagagccg caccgtcggc tacgccgccc
     8461 atggcgccgc cggcatgctg accctcttca tccgcggcgt gctgtgcaac tggatggtgt
     8521 ccaccggcgt cgtcgcagcc atgatgtcca cccacgtcac cggcaagatc ttcgccatgt
     8581 ggatgcccat catggtgttc ttctacatgg gcttcgaaca ctccatcgtc aacatgttcc
     8641 tgttcccgtc cggcctgatg ctggggggca acttctccct gtacgattac atggtgtgga
     8701 acgaaatccc caccgtggtg ggcaatctgg tgggcggcat caccttcgtc ggcctgaccc
     8761 tctactccac ccacgtgcgc acggccccca agcgcaacgc ctgatccgcc gtccctcccg
     8821 gcgtccgcct cgcgcggacg ccgcgccgga gccgtccatg ccgacctccc tcaccgtcac
     8881 cgtcgggcaa tactcggaca agggccgcaa gcccgccaac caggacttcc acgggctctg
     8941 catccccgtg gagccgcagc tcaccaccaa gggcatcgcg gcggcggtgg cggacggcat
     9001 cagcagcagc gaagtcagcc atctggccag cgaatcggcg gtcaagagct tcctcggcga
     9061 ctactactgc accgccgaga cctggagcgt gcgcacctcc gcccagcgcg tgctggaggc
     9121 caccaacgcc tggctccacg cccagacccg caacagcccc caccgctacg acaaggatcg
     9181 gggctacatc tgcaccttca gcgggctggt gctcaagtcc cacaccgccc acctcttcca
     9241 cgtgggtgac agccgcatcg cccgcctccg cgacggccgc ctggaggtcc tcaccgacgc
     9301 ccaccgcatc gtcgtctcgc ccgagcagag ctacctcgcc cgcgccctcg gcttcgaggc
     9361 tcaactggaa ctggactacc gccaggtccc catcgcggcg ggcgacgtct tcgtcctgag
     9421 caccgacggc gtgcacgaac acgtcggcgc cgacgtcatc gtcgccacgc tgcgcgcagc
     9481 ccccgacctg gacgccgcgg cccgccgcat cgccgacgca gcccttgccc agggcagtgc
     9541 cgacaacctc acgatccagc tcatccgcgt cgacaccctg cccgtccagg ccgccgccga
     9601 gcacctccag cagttcgccg aactgcccct gccgccgctg ctggacgccc gcatggaatt
     9661 cgacggctac cgcatcctgc gcacgctcca cggcagcagc cgcagccacc tctacctggc
     9721 cgaggacatt gccagcggca cccgcgtcgc cctcaagctc ccgtccatcg accagcgcga
     9781 ggacaaggcc taccgggagc gcttcatgct cgaggagtgg atcgcccgcc gcctcgacag
     9841 cccccacgtg ctcaagccct gcccggttac ccggcagcgc agctacctct acgtcgccac
     9901 cgagtacatc gaaggctcca ccctggccca gtggatgcgc gaccacccca cgccggacgt
     9961 ggagaccgtg cgcggcatcg tcgaacagat cgcccgcggc ctgttggcct tccatcgcct
    10021 ggagatgctc caccaggacc tgcgcccgga aaacatcatg atcgacacgc aaggtgtcgt
    10081 ccgcatcatc gacttcggcg ccacccacgt gaacggcctg caggaaaccg ccgccaacga
    10141 caccctggca cccatcctcg gcacctgcca gtacaccgcg cccgaatact tcctcggcga
    10201 gcccggcacg tcccgctcgg acctcttctc cctgggcgtc atcgcctacc agatgctctg
    10261 cagccgcctg ccctacggca ccgacgtgtc ccaggcgcgc acccgcagcg cccagaagaa
    10321 gctccgctac cgcagcgtcc tcagcgacac ccgcgagatc ccggcctggc tggacgccgc
    10381 gctggccaag gcggtccatc ccgatcccct gcgccgctac gcggaactga gcgaattcac
    10441 ctacgacctg cgccacccca atgcggccct cctgggccag cgcccgcccc ccctgatcga
    10501 gcgcaatccc ttgctgttct ggcagatcct gtgcgccttg cagaccgtgc tcctgatcct
    10561 gctgctggcg cgccgcagcc tgctctgagc ggctgcgccc cccgccgggc cgtttcaccc
    10621 tacactgcag gggcagcctc ccgatcagtc atccgatgcg ccttccccct gccctcaaag
    10681 ccctccttgc ccagctcgcc ggctgggccg tcgccatcgg gctgctgcgc gccggcctcc
    10741 tgcccggcca ggcctgggcc ctggtgctgg cccaagccat tgctgcggtg agcgccgccg
    10801 tcgccctgcg cagcgccccc tggtggcgct ggatccacgc cggctttacg ccgctggcgg
    10861 cgctggcgct gcaactgggt ctgcatccgg cctggtatct ggcggccttt gcgctgctgg
    10921 cgctggtgta ctggagcagc ttccgcaccc aggtgcccct gttcctgtcc aatccggcca
    10981 ccgcagcggc cgtcgcgcaa ctcgtccccc ccggtcattc cgcccgcgtg ctggacctgg
    11041 gcagtggcac cggcgccctg ctgcggcccc tcgcccgcct gcgcccggac tgccgcctgg
    11101 aaggcatgga aagcgccccg gcgccctatc tgctgtcccg cctcctgtcc cgccgccacc
    11161 ccaatatcgc cctggcgcga ggcgatttct ggcagatccc gtggaccgac tacgacctga
    11221 tctacgcctt cctgtccccc gtccccatgc ccgaggtgtg gcgcaaggcc tgcgccgagc
    11281 tgcgccccgg cgccctgctg gtgagcaaca gctttccggt cgagggcatc gagcccgacg
    11341 acatcatcca cgtcgccgac cgccgcggca cccgcctcta ctgctaccac cccgccggac
    11401 cgctgccgcc ggacggccgt cccgtcgggt ctccggtcgg tctcctcccc tgaggcacgc
    11461 tggtccggaa ttacccccgg aacgcccctc aattccaccc ctcgccgtgc cgttagggca
    11521 cgaataatgg cgccaccgcg ccgtgcgttc tgcgagaggt tttcaccatg tccacgatca
    11581 tctgcatcat cggcaacaag ggcggcaccg gcaagacgac cctgtcccac atgctctgcc
    11641 agggtttcgg gctgctcgga cagcgttccg cctgcgtcct caccgacgtt tcccgcgagc
    11701 ccctcagccc cgacgggcgg cgctacgtca ccgccgacgg gcgcagcccg gaagcccttc
    11761 acaaggtcgt cggcaagctg cgcaccatgc aggactggat cggcgtgatc gacggcggcg
    11821 gcaaccgcag cgaggtggac cgcctcctcg acagctacgc cgacattgtg ttgctgccct
    11881 tccgcgactc ccacgaagac atgcgcaccg tccgccgcga cctggaaacc ttcccccgcg
    11941 cctacggcct gccctcccag tgggccacca acgcctggca gcgcgacgcc gccgaccggg
    12001 ccgtgctcga actgctgggc gaccaccggg accgtctgct gcacccggtg gccagcctgt
    12061 ccgcctccaa gctgctgctc cagaagcaca tccccgccag tctgccctcc aacctgggca
    12121 acgcctgccg tgcgctggcc cgccaggtga tggacgtgct cgaaaccgac gccgacgccc
    12181 tgggcaccga tgcacacgaa gccgcccact cgctgcccgc ccccaccgtc ctgccggccg
    12241 gctacgccag cacggcccat cactgagtcc gccgcccccc gcatccgcga cggtccaccc
    12301 gatccgccgc gcccacctgc ggaacaggtc cgggtgacgt gcctgcgggc gtgacagaca
    12361 cccgcgcacg ccgcccgccc accccccggc ggtttaccgg ggtgcccacg tcgggcagaa
    12421 ttcgccccgg cctccacttt ccccccgatc acgatgctcc gtgcccacgg cctcgcctgc
    12481 gaccgcggtg accgccgcct gttttccggt gtcgactgcg ccgtcgcgcc cggtgaatgg
    12541 ctccaggtgg aaggccccaa cggcgccggc aagaccagcc tgctgcgcat cctcgccggc
    12601 cttgcgccag ccgccgccgg ccacatcgag tgggaaggcc ggccggtggc cgacgtgcgc
    12661 gacgaatacc acgcccgcct gctctacctg ggccacgcgc caagcatcaa ggacgacctg
    12721 agcgccctcg agaacctgac catcgccgcc gccgtggccg gccgacctct ggacgaggcc
    12781 gccgccctcg gtgccctgcg tcgcatcggc ctgaaggggc gcgaagacct gcccagccgg
    12841 gttctctccc agggccagaa gcgccgcgtc gccctggccc gcctgctgtg cagccaggcc
    12901 cgcctgtgga ttctcgacga acccttcgtg gccctcgacg tggcagcggt ggaccagctc
    12961 tgcgccatcc tcgacgacca cctggggcgc ggcggcatgc tgttgttcac cagccaccag
    13021 gcggtgcagc tcagcggcgc cggcggcagc ctgaggctgg gcgcatgaac gccttcatcg
    13081 ccatcgtccg ccgcgacctg ctgctggccg cccggcgcaa gagcgaagtg ctcaccgccc
    13141 tgttcttctt cctcatcgtg gtgagcctgt tcccgctcgg catcggcccg gaaaccgcca
    13201 tgctgcggcg catcgccccc ggcgtgctgt gggtggccgc cctgctcgcg gcaatgctgt
    13261 ccctgcaccg cctgtttgcc gccgaccacc aggacggcac tctggaacag atggccctgt
    13321 cgcctgtgcc cctggccctg ctggtgggcg gcaagatcgt cgcccactgg ctcacctccg
    13381 gcctgcccct ggtgctgctg gcaccggtgc tggggatcca gttcgacctg gcgccccagg
    13441 cgctgggcat cctgatgctg tccctgctgc tggggacgcc ggtgctgtcc ctcatcggcg
    13501 ccatcggcgc agccctcacg ctgggggtgc gcggcggcgg cgtgctgctg tccctgctgg
    13561 tgctgcccct gtatattcca gccctgatct tcggtgccgg cgcggtcgag gccgagatgg
    13621 ccgggcttgg cgccggcggt cacctgtccc tgctgggcgc ggtgctggcc ctcggcgcgt
    13681 tcttcgcccc gtgggcggcc acggcggccc tgcgcatcgc cctcgaatag cgtttcctgc
    13741 ggaacccctc catgagtcag cgtctcatca actggttcaa gttcgcctcg ccggccagct
    13801 tctaccccct cgccggccgg gcctggccct ggtttgccgc cctggcggcg gcatttgcgg
    13861 cggcagggct gtggctcggc ttcttcgtcg cccccaccga tgcccagcag ggcgaaggct
    13921 accggatcat cttcatccac gtggcggcgt catggatgtc catgttcatc tactgcgtga
    13981 tggctttctg gtcggcattg ggcatctccc tcaacacccg cctgtcgggc atgatgtgtt
    14041 ccgccctggc gcccaccggc gcgctgttcg ccttcctgtc cctgtggacc ggggccctgt
    14101 ggggcaagcc catgtggggc gcctggtggg tgtgggacgc gcgcctcacc tcggagctga
    14161 tcctcttctt cctgtacctg ggctacatcg ccctcaccag cgccatcgac gacccgcgcc
    14221 gcgccgacaa ggccggcgcg atcatctccc tggtgggcgt gatcaacgtg cccatcatct
    14281 atttctcggt gaagtggtgg aacaccctgc atcagggcgc gtccgtgtcc ctcaccaagg
    14341 ccccgtccat ggccaccacc atgttgtggg gcatgctgct gatggccctg gccttctgga
    14401 tgtacagcat cgccaccgcc ctgtatcgcg tgcgcgccat catcctcgaa cgggagcgcc
    14461 acgccgcctg ggtggccgaa ctgccggaga tgcaggcatg aactggggca gcgcgagtga
    14521 attcttcgcc atgggcggct atggcctcta cgtgtggggc agcttcggcg tcacctttgg
    14581 cgccctgctg atcgaagccc tgctgacccg cgcccgcttc cgggccgccc gccgcgccct
    14641 gcgccgggaa ctggccgccg acagagagag tcaagatcac ccatgaaagc ccgacacaaa
    14701 cgcttcgcca tcgtcctcgg cggcctcgcc gcgctgggca tcgccgccac cctggtgctc
    14761 aacgccttcc agagcaatct ggtgttcttc ttcaccccca cccaggtggc cgccggagag
    14821 gcgcccaaag accgggcttt ccgcatcggc gggctggtga aggagggcag cctgcagcgc
    14881 acggacatca ccgcccgctt caccgtcacc gacaccgcaa aggaaatccc ggtgacctac
    14941 accggcatcc ttcccgacct gttcagtgaa ggcaagggcg tggtggccca gggacgcctg
    15001 ggggtggacg gccagttcac cgccagcgaa gtcctcgcca agcacgatga aaactacatg
    15061 ccgccggaag ccaaggcggc cgtggaccag gcccaccagg ccgccaagtc cctcaagcag
    15121 taagcccgga ggcatcctca tgatcccgga actcggcagt ttcgccctcg tcctcgccct
    15181 gctggtcgcc ctcgtccagg gcgtgctgcc cctggtaggc gcccagcgcg gcatcccggc
    15241 gtggatcgcc ctcgcccgac cggcgtcggc caccctgttt ctgctggtcg ccgtggcctt
    15301 cagctgcctg acggcgagct tcatcaacaa tgacttctcg gtgctctacg tcgtccagca
    15361 ttccaactcc aggctgcccg tcgaataccg catcgccggc gtgtggggcg gccacgaagg
    15421 ctccctgctg ctgtgggtgc agatgctggc cctgtggtcg ctggcggtgg cggccttctc
    15481 gcgcagcctg ccggaggaca tggtggcccg cgtgcaggcg gtcctcgggc tggtggcggc
    15541 cggcttcatc ctcttcatcc tgaccacctc cagccccttc gagcgcctgc tgccgggcgc
    15601 cgaagacggg cgtgacctga acccgctgct gcaggacccg ggcctgatct tccacccgcc
    15661 catgctgtac atgggctacg tgggcttctc ggtggccttc gccttcgccc tcgccgcgct
    15721 cctgtcgggt cgcctggatg cggcctgggc gcgctggtcg cggccgtgga ccacggcggc
    15781 gtggatcttc ctcaccctcg gcatcggcct gggctccatc tgggcctatt acgaactggg
    15841 ctggggcggc tggtggttct gggatccggt ggagaacgcc tccttcatgc cctggctggt
    15901 gggcacggcc ctgatccatt ccctcgccgt caccgaaaag cgcggcagct tcaagaactg
    15961 gaccgtgctg ctcgccatcg gcgccttctc cctgtccctg ctgggcacct tcctggtccg
    16021 ctccggcgtg ctgagttccg tgcacgcctt cgccgccgac cccaagcgcg gcatcttcat
    16081 cctggccttc ctggcggtgg tcattggcgc ctccctcacc ctcttcgcct ggcgcgcccc
    16141 caaggtcggc ctgggcggcg cctttgcgct ggtgtcgcgg gagaccctgc tgctcgtgaa
    16201 caacgtgctg ctcctcaccg ccgccggcgc cgtgctgctg ggcaccctgt acccgctgat
    16261 catcgacgcc ctcaacctgg gcaagctgtc ggtcggcccg ccctacttca acacggtctt
    16321 cgtgcccctg atggtgcccg tgctcttcct gatggcagcc ggcccggtgg cgcgctggaa
    16381 gcaggccgat ctcggcgaga tcgcccgccg cctgcgctgg gcctttggcg tggcggtggc
    16441 ggccggcgtg agcctgccct tcgtcagcgg tggctggtcg gccggcgtcg ccctgggcct
    16501 cgccctggcc ctgtggatct tcggctcggc catcgtgcag atccgtgagc gcctcaagac
    16561 cggccggccc ccccgcgcgt tctggggcat gcacctggcc cacgtgggcg tggcggtgac
    16621 cgtgttgggc atcgtcatgg tcaagaacta cgagtcggaa cgggacgtgc gcatggcccc
    16681 cggcgatacg gtcaaggccg gcgactacgt gctgcgcttc aaaggtgtgg aatccgtgcc
    16741 gggccccaac tacacggccg cccgcggtga cctggacctg atccgcgacg gccaggtggt
    16801 gctccagttg cagccggaga agcgcaagta cctctcctcc gccatgccca tgaccgaggc
    16861 cgccatcgat tccggcctga cccgcgacgt ctatgtctcc ctcggcgacc cgctggacgg
    16921 cgaggacggc gcctggagcg tgcgcgtgta ttacaaaccc ttcgtcgcct ggatctgggc
    16981 cggcgcgctg ctgatggccc tcggcggcct gcttgccgcc agcgaccggc gttaccgcct
    17041 caccgccaag gctgcccggg ccaccgctct ggccggccag ggagccgcct cgtgaaacga
    17101 ttcctgattc cccttgccct gttcctcgtg ctggtgggct tcctcgccat cggcctcaac
    17161 cgcgatcccc acgaggtacc ctcgcctctg gtaggcaagc cggcaccgga cttccgcctc
    17221 gaggtgctgg ccgagccggg ccggaccttc acccccgccg acatgcgcgg caaggtctgg
    17281 ctgctcaacg tgtgggcctc ctggtgcgtc tcgtgccggg ccgagcatcc gctgctggtg
    17341 gccttcagca agcagggcgg cgtgcccatc gtgggcctca actacaagga ggtacgcggt
    17401 gacggccaga ccgacgtgaa gggcctcgcc cccgccgacg aacaggccct cacccgccag
    17461 cgcgccgccg aatggctcgc gcgccatggc aacccctaca gcctgtcggt cctcgacatg
    17521 gacggtcggg tgggcatcga ctacggcgtg tatggcgtgc ccgagaccta cctgatcgac
    17581 aagaagggag tgatccgcta caagcagatc ggccccatca cccccgaggc gctcgaacag
    17641 aagatcctgc ccctcgcccg ccagctggaa caggaggcct gaggtgacga tgccctcccg
    17701 cgcgcagcgc gtgcgccccg ccatcacgat gcgcggtcgc ctcctcgcga ccgccgccgc
    17761 cctgctcgtg gccggctcgc cggtcctcgc cgccgacgcc cgccccctcg ccgacgaccc
    17821 gatcgtcgag gcccgcatgc tgaccatctc cgaagaactg cgctgcctgg tctgccagaa
    17881 cgagaccctc gccgcctccc gcgccgagct ggccgaggat ctgcgccgcg aggtgcgcag
    17941 cctgatccgc cagggcaaga gcgacaccga gatcaaggac tacctcgtga cccgctacgg
    18001 tgacttcgtc ctctaccgtc cgccggtcaa atccaccacc tggctgctgt ggttcggccc
    18061 cttcctgctg ctggccggcg gcgtggccgg cctcgtcgcc tatctgcggc gccgcgccgg
    18121 acgcgtcgcc ccgcccaccc tgtccgccgc cgaccgggcc gccgccgaag ccctgctcgc
    18181 cgggtcggcc gacgcgccgg ccggctccac ctcctcgccg aaagaccagg catgaccgcc
    18241 ttctggatcg ctgccgggct gctcaccgcc ctcgtcctcg ccctcctgtg ccgcccgctg
    18301 ctgcaccgtc gcgatgccac cggggcctcc cgcaaggccc tcaacgccgc catctaccgg
    18361 gaccagatgg ccgaactgga gcgggatctg cagagcggcg tcctgtcccg cgacgactac
    18421 gccgtcgccc gtgacgagct ggagcgccgc gtgctcgaag acgtagccgc cgacgacacg
    18481 ccgaccgaca cccgccgacg cctgccccgc agcgccgtcg gcctggccgt gctcctcccc
    18541 gccgcggccg tggggctgta cttcgtcctc ggcacgcccg ccgccctcga tcccgcccgc
    18601 gtggccggca ccgagcaggc acccagccag gccgacatcg agaagatggt cgccgccctt
    18661 gccgccaagc tcgacaagga gccgaacaac ccccagggct gggtcatgct cgggcgcagc
    18721 taccgcgtga tgggccgcct ggatgaggcc gagaaggcct ttggacgcgc cggtcccgtc
    18781 atggaatcgg aaccggcgtt gatgctggaa ctcgccgaac tctccgccga ccgcaacgag
    18841 ggccgcgtcg aaggccacgc ccgggagctg ctcctgcggg tgctcaagga cgcaccggag
    18901 aatcctcagg ctctggtcat cgccggcacc gacgccttct tccgcaagga cttcgccgcc
    18961 gcgatccgtc actgggaaaa agtgctggca ctcgtgcctc ccgactccga agacgccacc
    19021 aacctgacag ccggcatcga gcgcgcccgt gcccagctcg gccagggcac agcggccgcg
    19081 cggcccgcca cggcgacgcc cgtcaaggac gccgcggcca gcgcggtgaa aggccgcgtg
    19141 gagctcgcgc cgggcgtcca cgccgcgccg gacgataccg tcttcatctt cgcccgcgcc
    19201 ttcgatggcc cgcgcatgcc cctggcggtg atccgcacca aggcctccgc gctgcccatg
    19261 gacttcaccc tcgacgacag cctcgccatg agcccggacc tgcgcctctc cggcgccgcc
    19321 cagctgcgca tcgaggcccg ggtgtccagg tccggcgacg cccttgcccg gccgggcgac
    19381 ttcaccgcca gcgtcggccc cgtcaagccc ggcacccgcg gcctcaggct cgtcatcgcc
    19441 gatccggtga aatagcagcc cctgccccgc agcacgcgct cagccagccg ccgccttcag
    19501 ccccgcggcc acttcgcagg ccggcgtgta gaagcggatc gtgttgcgtc cgccagcctt
    19561 ggcctcgtac atggcgatgt cggcgcgctt gatgatgtcc tcccgcgaca gctcgtggcc
    19621 gcggaacaga ctcacgccga tgctcgccga acaattgaag gtgacgtgcg tatcgccgtt
    19681 gcgcccccgc agctcgtaag gcgccaccag ggcctcgcgg atcttctggg ccaccatttc
    19741 ggcctgggcc gccgccgcgg gttcgtcccc caggtgctcc aggatcacca cgaattcgtc
    19801 gccccccagg cgcgccaccg tgtccgactc ccgcacgcag acgctcaggc gctgggccac
    19861 cgtcaccagc agctcgtcgc ccagcgcgtg gccatagcga tcgttgaggc tcttgaagcc
    19921 gtccatgtcg aggaacagca aggcacagcc ataaccggcg cgggcactgg cgacggccgc
    19981 ctgctccagg cggtcgtaga acaggcgccg gttgggcagt tgggtgagcg gatcgtagaa
    20041 ggccagacgg tggatctgct cctcgctctt tttgtgttcc gtgatgtccg agaacgccgc
    20101 cacgtagttc gtcacgcgac ccgccgtatc ctgaaccgcg gagatcgtca cccattcggc
    20161 gtagatctcg ccgttcttgc gtcgattcca gatctcgccc tgccagaagc cgtccttcgc
    20221 caggctctca tgcagatgac gatagaaatc cgcactgtgc aggcccgaac gcaacaggga
    20281 cggacgctgg ccgaccgcct cttccacgga atagccggtc atcgccgtga aggcctggtt
    20341 gacccgcacg atgcgcttct ccgcatcggt gatgaagatg ccttcctggg tctcgaaggt
    20401 ggccgcggca atgcgcaact cactctcgat ctccttgagc ccggtgatgt cggtgacgaa
    20461 gccgtgccag ctcaggcccg cagcgtccgg ttccggcatg gcgtccagga gcagccagcg
    20521 cacgcctgcc tccgggtggc ggatccggaa ctcgcaatgc cacggctcca gcgcctcggc
    20581 agagcggtcg cgggaggcaa agaatgcctc ggcatcgtcc gcatgcagga gggcgtggaa
    20641 gtcgtccgcg gaatgctcga cagcgctcgc ctccagcccg cagatctcgc gcagggcatc
    20701 gctcgcgaag ggcagcgcgt agcggccgtc gtcgtaccgc tgatactgga tcacgatgcc
    20761 cggcacccgc gacacgatgg cccgcaggcg ctgattgaac agatgctccg ccgcccgtgc
    20821 ctcctccgcc acgatggccg ccagcagccc gttgaaggcg cccgccaggg caccggtttc
    20881 atcgtcgcgc cggaccggca gcggctggcg cggaatcaga ccgtcgcgca tctgccccag
    20941 catgctggcc gcctcgccga gagggcgcag ctcgtgacgc agccaccaca acaccagccc
    21001 ggccgccagc agacccaccg ccaccgatgc cacaacgatg cgctggagca cctcgtcgat
    21061 gggcgcaaag gcctcttcgg aaggcagcac cgcctgcatc agccagcccg tggacggcac
    21121 ccgcttgctg gatgacaact ccaccacccc ccgcgaactg cgcgccaccc ccgagccgtc
    21181 gtagccttcc agatagcggt cgaacaccgg gttgacgccc accggcggcc ccgcctgcat
    21241 cacccgtgtc ttgtcggttg cggcgatgaa gatgcgctgc tcaggatcgg tgatcagata
    21301 gccgccacgc cgcccgaaac ggttcgaact gatgtcgtcc aggaaattgg ctgccgccag
    21361 gttggtgagc ccgatcaggg tgccactgat ccgcccgtcc cggtcgcgca cagggaccac
    21421 catcggaatc accggcttgc cggtggtctt gccgaacacg gcccggccaa tctgggcgcg
    21481 cccttcggac accgccagcc ggatcgactc cacctcgcca taattcaccc cctgccgccc
    21541 catggattcg ggcaggctca ccagcgccgt cccctcccgg tcggtcacca gcacacccca
    21601 gttgaacagg gacagcaggg ccggctgttg cctgaggacg tcggacggcg taccggacgc
    21661 gccccgcccc gcctgctgca gcacctcggc aaccgcctcc agggcctgca aacgctcgcg
    21721 caccgaacgg tcgatgtcgt cggcgagcag cgataccacc gagaactgct gggccgacag
    21781 ggtcgcctcc atctcccggc ggagaaaata atccagcgtg acgtaggtga gccacagggt
    21841 cacgaagacc ccgcccagca cacctgccgc aatgcgcgcc cgcagggacc gtcgaaactg
    21901 cgcaaacatc ccggtcacct tcacgttcca gctctccgga ccactgctcc tcctgctcac
    21961 ggcgggccgg ggcccgcctc tcgtcgtcgc ggccgatgct tgccgaccgc gttgttgtac
    22021 attgttccac cacagacgac tatatgcctc tcgacgtagt cgccctgcgg gcggattccc
    22081 cattccgggc acctgtccgg gccggacggg cccgcaggcg agcacgtccg ccccttcaac
    22141 ccgacgggac atccatgaca tcgagcacgc cgcccccgga tcctggccgc cccccgacgg
    22201 tccgcaacct ctttgccgac cgcccgtccg ccggcccgga cgaacacttc gacaccctgc
    22261 tgaaccatcc ccaggcacgc atcgaacgga tcctgtccca tcggcatgcc agcccaccgg
    22321 acttctggta cgagcagccg ggcgacgaat gggtccttct cgtccacggc agcgcaaccc
    22381 tcgaattcga caacgggcag cgcacggaac tgcggcccgg cgactggctc accctgcccg
    22441 ccggctgtcg tcaccgggtg gcggccaccg ggccggacac cgtctggctg gccgtgcatc
    22501 tgtcacgcgg atcggttagc ctaagcggat agattcactc ctcgggaaac caccatgatc
    22561 cgtcgtcgca tcgcccttct cactttcagc gcccttgcct gcaccgccgc cgtggcccct
    22621 gtcggcccgc tggtcggcgc ggcccacgcc gaggcgccgg ccgtgcgcac ccaggtcccc
    22681 ggcttttacc gcatggccct gggcgacgcc gaagtcaccg cgctctatga cgggtatatc
    22741 gacctggaca cgaaaatcct cagcaacgcg agccccgccg agatccagcg cctgctggcg
    22801 cgcatgttca tcgccggcga gaaggtccag accgccgtca acacctacct ggtcaatgtc
    22861 ggcggcaagc tgatcctcat cgacaccggc acggccaagg ccttcggccc caccctcggc
    22921 ttcgtcgcgg accacctgcg cgccgccggc tacacgccgg agcaggtgga cgtggtgctg
    22981 ctcacccacc tccacccgga ccacgtcaac ggcctgctgg gggccgacgg caagcccctc
    23041 ttccccaacg cccaggtgcg cgtcgcccag gtcgagagcg acttctggct gtccgccgac
    23101 aacacggcca aggcccccaa agacttccaa cccttcttca agatggccca ggccgccgcc
    23161 gcgccttacc aggcctccgg ccagtggaag cccttccacg acgaatccga agtcgcgccc
    23221 ggcatcaagc ctgtccccgc ggtcggccac acaccgggcc acagtgccta tgtggtggaa
    23281 tcgaagggcg agcgcctgct gatcctcggc gacacggtgc acagctacgc cgtccagttc
    23341 gcccggccgg aagtggccat cgagttcgac accgacaaga agcaggccgt cgccacccgc
    23401 aagaagctct tcgcctgggc cgccagggaa aagctcgctg tcgccggcat gcacctgccc
    23461 ttccccggcc tcggccacat ccgcagcgag ggcaaggcct tcgcctgggt gccggtggag
    23521 tacagccccc tgcgcgacag ggcaaagtaa gcccccgcgg tcgcgggcgg cgaaaaaacg
    23581 tcagacacag cggactggag gccggccccc ggtccgcttc accccgacgc accgcccgcg
    23641 atgcagccgc tccggtgcgc gccccacacg ggcgcgcctc aggtccgggc cagacagacc
    23701 agtgccgcgt tgtggaagcg cggaaaaccc agatcctcga tgctgtcgat ctcgcccagg
    23761 gacaaggccc cgacgatctg tgcggccccc gcgcgccccc tcaggcgctc gacctcgcga
    23821 ccgctttccg ctgccccgaa atgcatccgc cggccggcgc agtagaaagt cagcaacggc
    23881 gtctgcatgg tcccgggagc cacgttcagg ccacggacga tgccatcgag cagcgcatcg
    23941 tcggccgccg ccggcgcctc cagcacccgc aacaccgaat tagggggcac ctcgcccacg
    24001 cagacgacgg ccccgtcatc ggtgaagccc acggggatcc gcaccagcac gtcgaacatg
    24061 gtgatgaccc cgaggggaaa atggacggcg tactcgtaga aattcgcgtg ggtcagcgcc
    24121 acgccgaagc tcgcccagat cacctcccga tacaccgtga aggccgggcg gccatcgatg
    24181 cggtcgatac ggttgcccgt cgtggaggtc gcacgcatct gggtgccgct cacgggatag
    24241 ccgtggcgca ccaccgccgc catgtccggc ggcagacaca gtcccagcac cccgccagcc
    24301 acctcgcggg tggcatcgaa caggcaggga atgggctgga agcgctcact ccccgcattc
    24361 gccccggcat gccggggcgg cgcagggaga cggctgtggg cggtcatcat cagcgtgccg
    24421 atgttcggga ccagggcgtc gaagatcgtg aacatcaacg gtggcgcacg gccgtccgga
    24481 ctctggaggt gcgcgcccat cgccgccgca agacgctcca cgccgtccgc cgtcgcgaca
    24541 tcctccagca ggaaggtcgg cggcatgggg tcgaggcaca gcagccacgt tccgcgcacc
    24601 gcaaagccgg tcgccgtgac cagcgccggg aagaccgccc cgaccagcgg catcgccgcc
    24661 gcacgggcga tcgcctgcaa ggcggggacc cgatcgcggg aagcctcggc caccagggcc
    24721 agcacgccgg ccgccggccg cgacgcgcgc cagcgctgca ggcactccgc cgccccgtcg
    24781 agggcatcgt ccagcaggca gaggtcgtcg atcatcgcag ggcggtcatg gaagacatgg
    24841 catgcagtgg gtggcactca tgcgctaggc gggaggggtc gccaaagcgc tcgcggcgga
    24901 cgaaggccga cgcaccgcgg cttacagccc ggccgcttcc cgcagggccg ccgccttgtc
    24961 ggtcgtctcc caggtgaaat ccacctcgtc ccgcccgaag tgcccgtagg ccgcggtgcg
    25021 ggagtagatc ggccgcagca aatccaggct ggcgatgatg cccttgggac gcaggtcgaa
    25081 gtgggcctcg accagttcga cgattttctc gtcggagatc ctgccggtgc cgaaggtgtc
    25141 caccatcagg ctcaccggcc gcgccacgcc gatggcgtag gccacctgca cctcgcagcg
    25201 ctccgccagc ccggcagcga cgatgttctt ggccacgtaa cgaccggcgt aggcggcgga
    25261 acggtccacc ttggacggat ccttgcccga gaatgccccg ccgccgtggt gggccgcgcc
    25321 gccgtaggtg tccacgatga tcttgcgccc ggtcaggccg cagtcgccgt tgggcccgcc
    25381 gatgacgaag cgcccggtgg gattgatcag gaagcgggtc gccggcgtga gcatctcctt
    25441 gggcagcacc ggcttgatga cctcctcgat caccgcctcg gacagcgccg cgtgggaaac
    25501 gtccggatga tgctgggtgg acaccaccac ggtgtcgatg gccaccggct tgccgtccac
    25561 gtatttgacg gtgagctggc tcttggcgtc cggacgcagc cacggcaggc ggccgtcttt
    25621 ccgcacctcg ccctgacgct gcatgatgcg gtgggcgtag tagatcggga agggcatcag
    25681 gctgggcgtt tcattgcacg cgtagccgaa catgaggccc tggtcgccgg cgccctggtc
    25741 cagatccagg ccctcgccct cgttcacgcc ctgggcgatg tccgaagact ggcggttgat
    25801 ggcggtcagc accgcgcagc tcttgtaatc gaagccgatc tcggagtcgt cgtagccgat
    25861 gcgcttgacg gtctcgcggg cgatgtccat gtagttgatg tcggccctcg tggtgatctc
    25921 gccggagatc accaccagcc cggtggacac cagggtctcg caggccactc ggccgcgcgg
    25981 gtcctgggcg aggatcgcgt ccagcaccgc atcggaaatc tggtcggcga ccttgtcggg
    26041 atggccttcg gagacggatt ccgaagtgaa gagatattcc ttggccatgc actgctcctg
    26101 aaaatgaaaa atcccctgtc ctggcgtcgt agccgtctcg ggggatctcg gagcggcgac
    26161 gctttagcag tatttgtcgg cctgcgcacg gggtgtccgc acactgcacc cgccctgcaa
    26221 gttgtcggat taactcggcg gaatcacaat tatatcttcg cgccttccct cgtcaatttt
    26281 ccgccccgcc cgcgccgcgc gcccgaccgt ttccgatggc ttccctgttc ttctccctcc
    26341 tggcccggct gcccctcggg ctgctccagc agctcggcgc cgggctgggc tggctgactt
    26401 acgccctgtc tgggagctac cgtcggcgcc tgcgggaaaa tcttgagcgc gccctcggcc
    26461 ccgaggccgc cgcgcgcgtc cggcccgcag ccattgccga cgccggacgc caggccctgg
    26521 agctgccctg gatcctgctg cgcccccagg ccgacctggc ccgcaagatg gtggcggtga
    26581 gcggctggga gcacgtggag gccgccgagg cccgcggcgg cggcgtgctc ttcatcacgc
    26641 cgcacctggg ctgcttcgag atcactgccc agtacttctc cctgcacaag cccatcaccg
    26701 tgctctaccg gccacccaaa aaggccctgc tgcaacccct catcgacgcc ggccgcagcc
    26761 gcggcaacat gcacctggcc cccgcagata cggccggcgt gcgccgtctg gtcaaggcgc
    26821 tgcgcgctcg cgaagcggtg ggcatgctgc ccgaccaggt gcccggcgcc ggcgaaggtc
    26881 agtgggtgcc cttcttcgga cgtcccgcct acaccatgac cctcgccgcg cgcctgtccg
    26941 aagtcgccgg cgtgagcacc ctcttcgcgt gggccgagcg cctgcccggc ggacggggct
    27001 tccacctgca cctgcgcccg gccctgattc ccctcacggg cgacaccgcc gcgcgctgcg
    27061 ccgcgatcaa ccgggagatg gaagcccaga tccgcgcctg tccggcccag tacctgtggg
    27121 gctacaaccg ctacaaacga cccgccggcg cccccgcagc gccgcccacc gccgaggtgc
    27181 cccccgcatg agccgtcttc tcgtcgccct actgtggctg atccactggc tgcccctgtc
    27241 cgtgcaggcg cccatcgggc gcggactggg ccacctgctg tactggctca tcgcgccgcg
    27301 ccgcaaggtg gccctcacca acctgcgcct gtgctttccg gaactgtccg aagccgagcg
    27361 ggtggccctg tgcaaggaac acttcgccct ggccggacgc agcctgctgg agcgcggcat
    27421 cgcctggttc gcctccgagg agcgggtgcg gcgcatcgtg cgcctggaca acgagcccat
    27481 gctgcgccgc ctcatcgagg gcggccagcc ggtgatcctc ctcgcccccc acttcctcgg
    27541 cctggatctg ggcggttcgc gcatggccat cgccttcgac agcgtcagca tctacgccag
    27601 gcagagcgac ccggtcatcg accgctggct ctaccacggc cgcagccgct tcggaaacca
    27661 gctcctgctg gcgcgggacg agagcatccg cgcgtccatc aaggcgatga aggccctgcg
    27721 ccccttctat tacctgcccg acatggacca cggcgccgag gagtccgtgt tcgtgccctt
    27781 cttcggcgtg caggccgcca ccctcgccgc gctgccgcgc tttgcgaagc tgggcggcgc
    27841 cgtggtggtg ccctgcgtca cccgcatgct gccgggcgga ctggggtatg tggtggaact
    27901 gggcgagccc tggcaggatt tccccggccc cagcgtggaa ggcgacgcgc ggcgcatgaa
    27961 cgccttcctg gaagaccgca tccgcagcat gccggcccag tactactggg tgcaccggcg
    28021 cttcaagacc cgtccgccgg gcgagccgcc gatctactga gcgcgccccg ccggccgggg
    28081 cgccggctca ttccgtcgga tcgtccttgg ccgccagctc ggccagcacc agcggaaagg
    28141 ccgcgctgcc caccagggcc acgccggaca cgatcaacac caacccggcg ccggactcct
    28201 gcaacagggg gtggagttcc gcgccaaacc ccgccagcag cagggccccg gcgatcaggc
    28261 aggcaaggcc gggaacgccc atcagccagg cgccggccgg cagtcgcttg agccccatgc
    28321 tcacacccgc cggaagatca ggtcccacac cccgtggccg aggcgcaggc cgcgctgctc
    28381 gaacttggtg aggggtcggt aatcgggccg cggcgcgtag ccgtcggcgg tattggccag
    28441 cagcggctcg gcggagagca cgtcctgcat ctgcacggcg tagtcctccc agtcggtggc
    28501 gcagtgcagg tagccgccgg gcgccagacg cgaggcgatc agccggacaa agggcccctg
    28561 caccagacgc cgcttgtggt ggcgcttctt gggccaggga tcgggaaaat agatatgcac
    28621 gccggccaga gacgcttcgg ggatcatgtc ccgcagcacc tccaccgcgt cgtgctggac
    28681 gatgcgcaga ttgtgcaggc cgccctcgtc gatgagcttg cacaggctgc ccacgccagg
    28741 ggcgtgcacc tcgatgccga cgaaatcctc gtccggccgc gcccgcgcga tctcggcggt
    28801 gctggcgccc atgccgaagc cgatctccac gatgtgcggc gcggtgcgcc cgaacaccgc
    28861 gtccagatcc accggcgccg gccggtagtc caggcccacg cggggcatca tctcgtcgag
    28921 gaaacggtgc tgggcctcgg acatgcggcc ctgacgcagc acgaagctgc ggatggactg
    28981 ggaggtcagg cgcggcgcgg aggcgccctc cggcgcgtcg gccggctggg cgtcggcggg
    29041 gatgtgcggc gtgtcgttca tggatcgtgc gggcagacgc ggcgccacgc gccgcgggtc
    29101 atctggggaa agtcttcggt gcgcgtgggc cggcgctcag ctgccgatga ggccggtggt
    29161 gggcgagctc gggctggcgg tgtagaactt gcggggcatg cgcccggcca ggaaggcctc
    29221 gcgcccggcc tcgacaccct tcttcatggc ggcggccatg cgcaccgggt cctgggccag
    29281 ggcaatggca gtgttcatca gcaccccgtc gcagcccagc tccatggcga tggcggcgtc
    29341 cgacgccgtg ccgacgcccg cgtccagcag cagcggcagc ttcaccgcgt cgcgcaccag
    29401 ctgcacgttc cacgggttga gcacgcccat gccggagccg atcagcgagg ccaggggcat
    29461 caccgccaca cagccgatgt cttccagcat cttggcctgg atcgggtcgt cggagcagta
    29521 caccatcacc tcgaagccct ccttcaccag cgtgcgcgcc gcctgcaggg tctcgggcat
    29581 gttggggtag agggtgtggg gatcgcccag cacttccagc ttgaccaggc ggtggtcatc
    29641 cagcagctcc cgcgccaggc gcagggtgcg caccgcgtcc tcggccgaat aacagccggc
    29701 ggtgttgggc agataggtga attccgacgg cggcagcgcg tccagcaggc tgggcgcgtt
    29761 cggatcctgg ccgatgttgg tgcggcggat cgccaccgtc acgatctgcg cgccgctcgc
    29821 caacaccgcc tcgcgggtct gggcaaaatc ccggtacttg ccggtgccca ccagcagccg
    29881 ggaggaatag cgcttgcctg caatcgtcag tgcatcgtcc atgggaagat ccgtcgcgaa
    29941 aaattctggg gaatgaagcc ggagaggaag aacgccgggc cgcagaccgg gcgccgcggg
    30001 cctagccgcc gcccaccgcc accacgatct cgagccggtc accgtccgcc agcgcggtgc
    30061 cggcgtgcag gcccttgggc acgatgtcgc cattgcgctc cacggccacc cgcttgccca
    30121 ccagccccat gctcgtcagc agctcgaaca ccgtggccgg cgccggcagc acacgcgcct
    30181 cgccattgac catgagggac aggacagcgc cgccgggcgg ccgggaatcg gtaaacatcg
    30241 gcacagttta ccacgcccat cccggttgcc ccccggccgc cggatgcgta caatcctcgt
    30301 ccttcgagcg ggcgtcgtat aatggtaata ccctagcttc ccaagctaga gccgtgggtt
    30361 cgattcccat cgcccgctcc acatcgaatt ccggcaggtt gttgatctgc aagcaaaa
//
LOCUS       DAOQMM010000035        30399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_410_length_30399_cov_56.821942, whole genome shotgun sequence.
ACCESSION   DAOQMM010000035 DAOQMM010000000
VERSION     DAOQMM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 30399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30399
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_410_length_30399_cov_56.821942"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLH85_07920"
     CDS             complement(<1..458)
                     /locus_tag="PLH85_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNB64527.1"
                     /translation="MRTQYCGLVSAAYLDQIVTLCGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRPEMFQVAESVRNEFVLKITGKVRRRPAGTENSQLVSGEIEVLCHEIEVL
                     NVSATPPFQLDEENLSETTRLTHRVIDLRRPQMQKNMILRYRTAMAFRRFL"
     gene            complement(513..1154)
                     /locus_tag="PLH85_07925"
     CDS             complement(513..1154)
                     /locus_tag="PLH85_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNB64528.1"
                     /translation="MKKYFVTGLLIWIPLVITAVVLAWIVNTLDQILLVVPPSVRPEA
                     FIGFHVPGIGVVVSLLLILLTGLAAANFVGQHLVKFWEKLLSRIPVVKSIYYSVKQVS
                     DTLLSSNGQAFRKALLIQYPREGMWTIGFQTGIPGGDAAHHLGSDFVSVYVPTTPNPT
                     SGFFLMLPRRDAIELDMSVDEALKYIISMGVVAPPPHRAPAPSHAATPALQNP"
     gene            complement(1180..1443)
                     /locus_tag="PLH85_07930"
     CDS             complement(1180..1443)
                     /locus_tag="PLH85_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB64529.1"
                     /translation="MPIYAYRCNACGFQKDHLQKLSDAPLTTCPSCGADSYAKQLTAP
                     GFQLKGNGWYATDFKGGSSSASKAEPAKADAPATPCGGSCACH"
     gene            complement(1589..2437)
                     /locus_tag="PLH85_07935"
     CDS             complement(1589..2437)
                     /locus_tag="PLH85_07935"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNB64530.1"
                     /translation="MQIFTEPPVFTAVCVLLGLFVGSFLNVVIHRLPIMMEREWQAEA
                     AALRGEAGEGADPFNLATPRSRCPHCGHLISALENIPVVSYLLLRGRCRHCSAPISLR
                     YPLVEVICAMLSGITAWHFGFGAAGLGALAFVWAMIALTFIDLDTQLLPDSITLPLLW
                     LGLLLNLQQAFATLPDAVVGAAAGYLTLWSVYWLFKLTTGKEGMGYGDFKLLAAIGAW
                     FGWQILPLTILLSSVVGALVGVGLIVFRRHGREVPIPFGPYLAAAGLLALYWGRPLTD
                     AWLGGF"
     gene            complement(2459..3703)
                     /locus_tag="PLH85_07940"
     CDS             complement(2459..3703)
                     /locus_tag="PLH85_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB64531.1"
                     /translation="MATVPKKTSSRAPVTAKEFLFVWEGKDKTGKIIRGEMRATTPNV
                     VQTVLRRQGILAHKITKQAFARGKKITDKDIALFTRQLSTMMRSGVPLLQSFDIAIKG
                     CGNPSFARLLNEVRSDVETGSSLSQAFRRHPLYFDALFCNLVAAGEQAGILDSLLDRL
                     ATYKEKMLAIKSKIKSAMFYPIAVVVVAALVIAVMMLFVVPEFKKVFTSFGADLPAPT
                     MVVIAISDFFVEYWYLFFGILAGTVFGISFTYKRSQAMQIAVDRAILQIPVIGEVVRK
                     ATIARWTRTLSTMFAAGVPLVEALDSVGGAAGNIVYKTATKQIQSDVSTGTSLTVSMQ
                     NSLVFPTMVVQMVSIGEESGQLDSMLGKVAEFFEREVDDAVAGLSQLLEPMIMVFLGV
                     VIGGLVVAMYLPIFKLGAVVGG"
     gene            complement(3726..5438)
                     /gene="pilB"
                     /locus_tag="PLH85_07945"
     CDS             complement(3726..5438)
                     /gene="pilB"
                     /locus_tag="PLH85_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNB64532.1"
                     /translation="MSASPQVALSGLARALVQHGRLMESEALSCTTPSGNPNEFIIEA
                     IKRGLISAAEVARFSAATFGYPLLDLGSFDEAHFALDAIDRKLMAKHQAVPLGRRGNR
                     LTLAMADPSNLRVLDEIRFQSGLAVDPVVVEAPKLVELVERLSESASTVLKDMTGDEF
                     DMDLLGKEGASEAQIEDVNSEVDDAPVVRFIQKLLIDAINEGASDVHFEPYEKYYRIR
                     FRTDGILREIAQPPLVLKEKIAARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRV
                     STLPTLHGEKIVMRILDPTSAMLGIDALGYDPDQREALLAAVERPYGMVLVTGPTGSG
                     KTVSLYTCLNILNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFASALRAFLRQDP
                     DVIMVGEIRDLETAEISIKAAQTGHLVLSTLHTNDAPSTLERLRNMGVAPFNIASSVI
                     LITAQRLARRLCSCKQPMDIPIEALLTAGFTEQDLDGTWQPYGPKGCERCKGSGYKGR
                     VGIYQVMPISEEIARIIMTNGNSMDIAAQAQLEGVKDLRQSGLLKVKQGVTSLTEVLA
                     CTNE"
     gene            complement(5528..6802)
                     /locus_tag="PLH85_07950"
     CDS             complement(5528..6802)
                     /locus_tag="PLH85_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HNB64533.1"
                     /translation="MDSIPLSVQSAILAALLALSGFFSMAETAMMAANRYRLRTLAKQ
                     GSRGARLALDLLGQTDKLLGIILLFNNLINAASATLVSVITIQLFGNEEWALGVSTLG
                     VTFLILVFSEITPKVIGANHADHMACVVSYPLSILLRGLYFVIWFVNLFVAGLLRLFR
                     LQSNGEGENQPMSTEELRTIVLEAGSFIPPKHRSILVNLFELEDITVEDVMIPRHQIE
                     ALDLSQPRDVILHQIATCYHTRIPVFDAHMDAVAGVLHVRRILAQSLEHPVDAADIRG
                     MLSPPYFIPASTPIYSQLQFFQENRERIGLVVDEYGELLGLVTLEDIIEELIGKFTTS
                     APDASDKLAWNKDGSVIADGSQTIRTLNRKLGLALPVDGPKTLNGLLLEHLQDIPESG
                     VSVRIAGVPIEVIQAEDRRIKNARLFRPRSAG"
     gene            complement(6856..7683)
                     /gene="ccsA"
                     /locus_tag="PLH85_07955"
     CDS             complement(6856..7683)
                     /gene="ccsA"
                     /locus_tag="PLH85_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNB64534.1"
                     /translation="MPVILLHLLPASLYALLGLHFWRSRWLQPALGPRQELLPWERGA
                     LLAALGAHGFALHHAFFASNGMVFGFSLALSLMLWLAVLFYWIESLYARLDGLQALAM
                     PAAALCALLPAFFPEQHVLENAHSPLFRAHFVVAMLAYSLFTLAALHALLMSVAERRL
                     HGARLSRAFASLPPLLTMEALLFRLIGIAFVLLTLTVGSGVLFSEVLFGRAFRFDHKT
                     VFAILSWLIFGGLLIGRHTRGWRGRKALRWTLAGFVALMLAYVGSRFVAEVLLGRAA"
     gene            7722..>9055
                     /gene="ffh"
                     /locus_tag="PLH85_07960"
     CDS             7722..>9055
                     /gene="ffh"
                     /locus_tag="PLH85_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNB64535.1"
                     /translation="MLDNLTTRLAKVVKTLRGQARLTEDNIQDALREVRMALLEADVA
                     LPVVKDFIARVKEKAVGTEVISSLTPGQALVGVVHRELTELMGGAHTGLNLATQPPAI
                     ILMAGLQGAGKTTTTGKLGKLLVERMKKKVLAVSTDVYRPAAIAQLQTVAQQAGVEFF
                     PSTPDQKPVDIALAAVDYARRHYMDVLLVDTAGRLAIDQAMMDEIKALHAAVNPVETL
                     FVVDAMLGQDAVNTARAFNEALPLTGVVLTKLDGDSRGGAALSVRHVTGKPLKFAGVG
                     EKLAGLEEFHPERMASRILGMGDILSLVEDARRGVDEEKAKQFAQKLKSGKGFDLNDF
                     KDQIGQMRKMGGLSSMLDKLPAQFAQAAGQLQGGVEEKAIRRIEGIINAMTPLERTKP
                     ELIKASRKRRIATGAGVTVQEVNRLLSQFEQTQKMMKQFSKGGMQKMMRAMKG"
     assembly_gap    9056..9155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9277..10113
                     /locus_tag="PLH85_07965"
     CDS             9277..10113
                     /locus_tag="PLH85_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNB64536.1"
                     /translation="MPETLYGRAYTALMTVDPAVKAARVAVLVADWQAGRLPAADGRD
                     APVLAVPDAGRPARPALLPHTDLPRRKPTTPEGHAALLHAIAHIEFNAINLALDCVYR
                     FRSLPDEFYAGWLQVAAEEADHFARVCTRLAALGHAYGDFPAHRGLWDMAVKTAEDPL
                     ARMALVPRVLEARGLDATPPIVAALHRIGDADSIAVLDIILRDEVGHVALGDRWFRHL
                     CAERGLEPEATYLALMADFDAPWPPGPLNVEARRRAGFSAAEIVRLEAGRPRGKVPQS
                     EP"
     gene            complement(10098..11237)
                     /gene="ccsB"
                     /locus_tag="PLH85_07970"
     CDS             complement(10098..11237)
                     /gene="ccsB"
                     /locus_tag="PLH85_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNB64537.1"
                     /translation="MDIAHPDTPSLLRRLQPGDWLYALAVAGGGMFALRQYGGFMDVY
                     DMAILALVVPAVIAVGWRWKPFRLFLAAVAALALTSVGLYEGDLARAEKIFLLKYFLS
                     SQTAIMWMSALVFVATGSYGLGLAARSDFAEKTGSALTWTSATFGTTGLFVRWYESYL
                     IGRDVGHIPLSNLYEVFVLFTLLTSLLYLYYEGRYATRRMGAFVLLVISAAVSFLLWY
                     TFSREAQEIQPLIPALQSYWMKVHVPANFIGYGAFALAAMIGVADLLVRRGLLASRLP
                     APEVLDDVMYKAIAIGFAFFTIATILGALWAAEAWGTYWQWDPKETWAFIVWLNYAAW
                     LHIRLTKGLRGALLAWWAVVGLLVTAFAFVGVNMFLSGLHSYGSL"
     gene            complement(11243..13297)
                     /locus_tag="PLH85_07975"
     CDS             complement(11243..13297)
                     /locus_tag="PLH85_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNB64538.1"
                     /translation="MQRSTLRKTYELLSSMRFAISLLTVLAIASIIGTVIKQNEPYNN
                     YLNQFGPFWFPVFEKLGLYAVYNAAWFLLILGFLVLSTGACIVRQTRPMLKDMRSFRE
                     HARETSLAAFQHHALLAVGLPFDEAARFTREHLIRNGFRFRENVRDDGVLFAGRQGSW
                     SRIGYFLAHAAILLICLGGLLDGNLPLKVQMALGGKHTTSGNQLIADIGPESRLGRDN
                     WSFRGNVFVPEGKSSNVAVLNVKDGILLQDLPFTVSLRKFTLEHYDSGMPKRFASDVL
                     ITGADGKTYEKTIEVNKPVEVDGIMLYQASFEDGGSRLRLLARDLRVDGTPDQSVDVA
                     VGDSLPLKRNGLDLTLELIAFRPFNVENLADPDTGAASGTLGTLEKHLGSGARSTAPK
                     DLHNVGPSFQYKLRDNTGQAREYQTYMQPIAQDGAWYLLAGMRDTPDAPFRFMRIPVD
                     ADQKADAWFALRRVLLDPRRHDELARRFAATALDRGDDAALRERLQETARRTLDLFAR
                     GGFESVGKFIESTIPEGERDRAAGVFIKVLEGVTWEAWQLARQDAGLPPLKNDAQYAR
                     FVRDSLNAASDSQHYGAPLLFQLDRFDEVRATVLQATRSPGKPIVYLGSLLLVLGVFA
                     MLYIRERRLFVLVKHSGEALVALSSNRKSLDVDTAFAAHRDALATRFTPPADAPPAIE
                     PR"
     gene            complement(13414..14049)
                     /locus_tag="PLH85_07980"
     CDS             complement(13414..14049)
                     /locus_tag="PLH85_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB64539.1"
                     /translation="MIKRSLLLSLLLAATAVQAEEKAPAQPDLAKAKMTAEQVCGACH
                     GADGNSQIPTNPKLAGQHPEYLAKQLKNFKSVDGKPAERANPVMGGMAAPLSDDDITG
                     LAAYFGSQTLKPEAAKNKASIELGQKLWRAGDLKRGIPACAACHGPAGAGLPAQFPRI
                     GGQFADYTEAQLKAFRAGERANDPAKMMRTIALKMTDPEIKAVSDFAAGLR"
     gene            14182..14790
                     /gene="yihA"
                     /locus_tag="PLH85_07985"
     CDS             14182..14790
                     /gene="yihA"
                     /locus_tag="PLH85_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNB64540.1"
                     /translation="MSLFRNARFEISIAKPSGLPPPNGAEVAFAGRSNAGKSSAINTL
                     ANHTRLAFVSKTPGRTQLINFFRLQNGAVLVDLPGYGYAQVPEAVRRQWQHLLENYLT
                     RRPNLIGLVLIMDSRHPLTERDQAMIGWFAPSGRPMHVLLTKSDKLSRNEAAATLTAV
                     RRELAPLGSQVTVQLFSSLKKTGIEEVEQVVGAWLAPAQDPA"
     gene            14898..16337
                     /locus_tag="PLH85_07990"
     CDS             14898..16337
                     /locus_tag="PLH85_07990"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNB64541.1"
                     /translation="MHLQPIILSGGSGTRLWPLSREQYPKQLLALVGHETMLQATAGR
                     LAGFAGALPVEQAPLVVCNEEYRFVTAEQLRACGRGGRDIVLEPAGRNTAPALTLAAL
                     AVEEADPVLLVMPADHVIRDLAGFQRAIATGIPAALAGAIVTFGIVPDRPETGYGYIR
                     FGAERNDGARSIAAFVEKPDAATAQRYLDSGEYLWNSGLFMMQASTWLKAMAHLQPAM
                     EAACRAAWAGGSRDADFVRLDKAAFLACPSDSIDYAIMEKLAGAEGLGIPGCVVPMSA
                     GWSDVGAWDALWQIADKDEAGNTARGDVLFENTRNTLVHADSRLVACVGLEDTIVVET
                     PDAVLVAHKERTQDVKQIVARLKADQRSQASAHRKIHRPWGWYDSIDSGERFQVKRIV
                     VNPGARLSLQMHYHRAEHWIVVKGTAEVTNGEKTFLLGENESTYIPLGHVHRLANPGK
                     VPLEIIEVQSGSYTGEDDIVRFEDKYGRG"
     gene            complement(16365..16880)
                     /locus_tag="PLH85_07995"
     CDS             complement(16365..16880)
                     /locus_tag="PLH85_07995"
                     /inference="COORDINATES: protein motif:HMM:NF022609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HNB64542.1"
                     /translation="MDDIVRQALLKWPDVPDCRGWLHLTRRGAWRVPTGIIHHAGLSA
                     FIGRNYGADETGRWFFQNGPQKVFVSLEYTPYVLRLRSDGSLETHTGLPVDALQAGWL
                     DDDGSLLLDSEHGIGLLDDRDLAAMAEGLEGNPDSAEPLHLRWRDTRVSIGRLKRAEV
                     SARFGFLAEPT"
     gene            complement(16911..17714)
                     /locus_tag="PLH85_08000"
     CDS             complement(16911..17714)
                     /locus_tag="PLH85_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNB64543.1"
                     /translation="MAHFTLRSLAFIGVLLLAGCSGLSDQADETAGWSAQKLYTEAKE
                     SMSDGAWDRAIKMFEKLEARYPYGRYAQQSQLEVAYAYYKSGEPASAVAACDRFIKLH
                     PNHPNVDYAYYLKGLAYFNEDLGLLGALANQDMSERDTKASRDAFDTFKDLVTRYPNS
                     VYAADAQARMRYLVNALAAHEVHVARFYLRRGAYVAAANRAQAAVKDFPESPSVEEGL
                     FLMIKAYDAMGLTELRDDADRVLRKNYPNSAFYSGQPRPGEKSAWWKLW"
     gene            17701..18681
                     /gene="rluD"
                     /locus_tag="PLH85_08005"
     CDS             17701..18681
                     /gene="rluD"
                     /locus_tag="PLH85_08005"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNB64544.1"
                     /translation="MKCAIDIPQEDDWLDYSPADPAPSPQRETVPADCAGLRADQALA
                     RLFPEHSRSRLQAWLKSGRIRIDGASPDAKCKVWGGETVEVSAEPTEESLAQQAEDIP
                     LAVVFEDDTLAVIDKPVGLVVHPGSGNWSGTLLNGLLHHDPAFRALPRAGIVHRLDKD
                     TSGLLVVAKTLEAQTDLVRQLQARSVKRHYYALVHGQPAEQGAVEAPIGRHPVQRTKM
                     AVVRGGREALTRYRVCERFRHATLVECELATGRTHQIRVHMAHIGHPLVGDATYGKAR
                     SADPRLDAFPRQALHAWQLGLIHPRSGEACVWQRALPADFETLLATLRSV"
     gene            18684..19430
                     /gene="pgeF"
                     /locus_tag="PLH85_08010"
     CDS             18684..19430
                     /gene="pgeF"
                     /locus_tag="PLH85_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNB64545.1"
                     /translation="MSNGNWIVPDWPAPACVRALSTTRLGGVSPAPWDSLNLGTHVQD
                     DPARVLANRAILRAQLPADPVWLNQVHGTTVVDAAVVSGIPDADAAIARASGAVCVVM
                     TADCLPVLLCDRAGTVVGAAHAGWRGLHGGVIEATLAAMGVAPHEVLAWLGPAIGPAA
                     FEVGDEVRAAFVADDEAAASAFLPGAAPDKWLADLYRLACQRLARAGVTRVSGGGLCT
                     VADRRRFFSYRRDGLTGRMASLVWLAAPDA"
     gene            19470..20261
                     /locus_tag="PLH85_08015"
     CDS             19470..20261
                     /locus_tag="PLH85_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNB64546.1"
                     /translation="MTPLAWIVLMSVGGGALSVLAASVVAYAARESWVPVLISYAIGA
                     LLGAAFLDVLPHAIESAATPTRAAATVLAGILLFFVLEKLVLWRHCHDEGCHGHGHDH
                     HHGHEGHDHGRSGLLILVGDTFHNFVDGVLIAAAFVDSTALGIVTALAIIAHEIPQEV
                     GDFLILLHSGFSRTKALAFNLLSSLATLVGGFLAYFALSQIESLVPVFLALAAASMIY
                     VAVADLIPGLHKRTELRATAQQVSLIAAGIATIAIAHPLIEGALG"
     gene            complement(20193..20417)
                     /locus_tag="PLH85_08020"
     CDS             complement(20193..20417)
                     /locus_tag="PLH85_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64547.1"
                     /translation="MYIVAIAWLYVALLATAAETTVVGGLLTFFFYGLAPLSLFLWLF
                     GTPARRRRSAQRALDEGMGDGDRGDTGSNQ"
     gene            20548..22287
                     /gene="phaC"
                     /locus_tag="PLH85_08025"
     CDS             20548..22287
                     /gene="phaC"
                     /locus_tag="PLH85_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNB64548.1"
                     /translation="MTPEQATEALQGMFKFGQSLAQNYFDFLGKQSSAMQQAAPVGAP
                     VPAVPLPEAQELARLQQELAESHARLWSTLAQTRPGEKAEAVAHPEPGDRRFSAPEWT
                     SSPIFDYIRQSYLLNSSFLNKVADALPVADGRAKARMQFLTRQYIDALAPSNFAATNP
                     EFIQTALDTHGESITRGIKNLIGDLEKGRISMTDDAAFEIGRNLATTPGSVIYENELM
                     QLIQYAPLTEQVYQRPLLIIPPCINKFYIMDLQPANSLVRFIVEQGFTVFLVSWRNAK
                     AEQAHHTWDDYLEKGPITALQVVREITQVDKPNVLGFCVGGTLLSSSLAVLRGRGEDP
                     AESLTLMTTLLDFGDAGELGCLVDETSVAAREAAIGKGGLLMGRELAQVFSSLRANDL
                     IWQYVVGNYLKGKTPEAFDLLYWNSDSTNLPGPFLAWYLRNMYLENNLRVPGKLEMLG
                     VKVDLGKVDLPAYLLATREDHIVPWTSAYLVRQILGGETTFVLGASGHIAGAINPASK
                     NRRSYWTSESAAAVPEEWLAAATERKGSWWLHWIEWLKTRSGEQIAARVKPGTKKYKV
                     IEAAPGRYVKEKV"
     gene            22411..23151
                     /gene="phbB"
                     /locus_tag="PLH85_08030"
     CDS             22411..23151
                     /gene="phbB"
                     /locus_tag="PLH85_08030"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNB64549.1"
                     /translation="MARVALVTGGMGGLGESICIKLAALGYKVATTHSPGNTKVGEWL
                     QTMNNMGYGFKAYPCDVADFDSCKACIEAVTAELGPVDVLVNNAGITRDMTFKRMTKG
                     DWDAVMSTNLDSVFNMTKQVMDGMVERKWGRVINVSSLNGIKGAFGQTNYAAAKAGMH
                     GFTKSLAQEVAKSGVTINTISPGYIGTKMVLAIPQEVLDSKILPHIPMGRLGKPEEIA
                     GLIAYLSSDEAAFVTGANISINGGQHMF"
     gene            23197..23937
                     /gene="phbB"
                     /locus_tag="PLH85_08035"
     CDS             23197..23937
                     /gene="phbB"
                     /locus_tag="PLH85_08035"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNB64550.1"
                     /translation="MTQRIALVTGAVGGLGTAICKSLAAEGIRVVGNFFPGDPAKDAW
                     VEARTAEGLDIPLYGFDVSDYEQCKAAVEKIVAEVGPIDILVNNAGITRDKFFPKMEK
                     GQWDAVIATNLTSLFNVTHTISPKMAERGWGRIVNISSVNGVKGQAGQTNYSAAKAGV
                     IGFTKALAAELATKGVTCNAIAPGYIGTDMVMAIKEEIRQGIIDTVPVKRLGKPEEIG
                     AAVVYLSSELAGYVTGATLNVNGGLYYQ"
     gene            24046..24651
                     /gene="phaR"
                     /locus_tag="PLH85_08040"
     CDS             24046..24651
                     /gene="phaR"
                     /locus_tag="PLH85_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNB64551.1"
                     /translation="MADQVRLIKKYPNRRLYDTQTSSYITLADVKQLVLEHGSFQVVD
                     AKTGEELTRNILLQIILEEEAGGAPMFTSDLLSQMIRFYGNAMQGMMGKYLENNIRAF
                     TDMQAKLQDQVRSVYGDNATVSHDLWAQFLNLQGPAMQSMMAAYMEQSKRMYAQMQEQ
                     LDSQTRNLFSGFQFPSFSGGFAPGKADADEAVAPPPAASKK"
     gene            24703..26034
                     /gene="rimO"
                     /locus_tag="PLH85_08045"
     CDS             24703..26034
                     /gene="rimO"
                     /locus_tag="PLH85_08045"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNB64552.1"
                     /translation="MTQSKQAGVPRVGFVSLGCPKALVDSEHILTRLRAEGYEISPSY
                     DGADLVVINTCGFIDAAVEESLDAIGEALNENGRVIVTGCLGAKEDVVMGAHPQVLAV
                     TGPHATEEVMQAVHKHLPKRHDPFVDLVPPQGIKLTPDHYAYLKISEGCNHRCTFCII
                     PSLRGDLLSRPIGDVMREAENLVNAGVKELLVVSQDTSAYGVDVKYRTGFWGGRPLRT
                     KLYDLCKALGDLGVWVRLHYVYPYPSVDDILPLMAEGKILPYLDVPFQHASPRILKAM
                     KRPASAENNLERVRRWREICPDLTIRSTFITGFPGETDADFEALLQFLEEAQLDRVGA
                     FAYSPVEGAAANELPGAVPEELREERRIQLMEFQEDISTARLERFIGREMTVLVDEID
                     EEGALARSPGDAPEIDGLVTIPAGEHLQVGEFARVRITDCDVHDLYAEPLE"
     gene            26047..26964
                     /locus_tag="PLH85_08050"
     CDS             26047..26964
                     /locus_tag="PLH85_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB64553.1"
                     /translation="MARRPPRIGIALGSGAARGWAHVGVLRALAREGIEPAVICGSSI
                     GAFVGGIAAAGDLDKLADWVDSLNWQQVVGLLDMGLRGGLIKGDKLIDFFQRHFVDHD
                     FASLRRPFGCVATDLQTGHEVWLRDGSVATAVRASIAMPGLMTPVVHEGRLLVDGGVV
                     NPVPVSLARAMGADLVIAVDLNADVVGRAWRAPNEPPPPPPEAGPFWARKLLTRFGLG
                     ERPAGGAGDDEGIDMPSMLSVIQGSISIMSVRIARSRLAGEPADVLIAPRMAQVGWME
                     FHRGREGIAEGEAAVELALPALRRALESF"
     gene            26975..28378
                     /locus_tag="PLH85_08055"
     CDS             26975..28378
                     /locus_tag="PLH85_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB64554.1"
                     /translation="MDAFLDAVRAIVGPTHCLCGEAAAPYLTDWRGRYTGSARAVAFP
                     ADTAEVAALVRVCVDADVPIVPQGGNTGLCGGATPLDDGRALLIQLSRLNRIRHVDGG
                     NNALTAEAGVTLAAVHAAAEAADRQFPLSLASEGSCEVGGVISTNAGGVQVLRYGNTR
                     ELVLGLEVVLPDGQIWNGLRALRKDNTGYDLKHLFIGAEGTLGIVTAATLKLFPRPVA
                     TRTVWLAIPHPMAAVALLGQMRARAGDRLTAFELVSRVSLDLVLQHMPGARDPLPTVH
                     PWYVLAELTDTVRDASLDAMAETLTAEVLESGLVQDGTVASSEAQARALWALREHISE
                     AQKREGYSIKHDISLPVSAIPDFLDDVAGRLEQVWPGLRIVCFGHLGDGNLHYNLSLP
                     VSADNAGLIARSSDVSGLVHDRVHRFGGSISAEHGIGQLKRGLLPRYKSEVELDLMRR
                     IKAAMDPAGRMNPGKVL"
     gene            28631..29473
                     /locus_tag="PLH85_08060"
     CDS             28631..29473
                     /locus_tag="PLH85_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB64555.1"
                     /translation="MSYMAKTKQLAVALGAALTIGGASVAHADVYEYANKFSANYVFS
                     GFSDAGTVGDDHTFTLSFSNVSGNLDATIPASSGYYSISRKGEFDFDYNGTSVPSTLQ
                     APSVCSGLGKANWDFCSKTTNYVPVGAGNFNWSGITGTHFLYNWDTDTFTSNGIPQTT
                     GDFAGNSTAIMLLLGTFLGPVGTTIATYVGDGNVFVHQVMNGAANTWTLTFTEDPGVG
                     IESVLKALDAGTIPGLPKALTNAMIDGVVYADGSLRVVPEPGSLALLGLGAAALAVRR
                     RKSA"
     gene            29674..30057
                     /locus_tag="PLH85_08065"
     CDS             29674..30057
                     /locus_tag="PLH85_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HNB64556.1"
                     /translation="MADKAKEAFKQRIREMTRRSGGRSLPEIVERLRAYLPGWKAYFQ
                     LAQMPGVFRELDEWIRHRLRAVQLKHWRRGTTMYRELKAMGASETDARQVAANSRRGW
                     RNSRFLLNRAMPVAYFDRLGVPRLS"
     gene            complement(30217..>30399)
                     /locus_tag="PLH85_08070"
     CDS             complement(30217..>30399)
                     /locus_tag="PLH85_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64557.1"
                     /translation="TADLLHAMQALSQLSYTPNQRAAHYKKHCTACKAPPLKIFLKQR
                     NDLRIIDSNNIESKLL"
BASE COUNT         5433 a         9406 c        10209 g         5251 t
ORIGIN      
        1 aggaagcggc ggaaggccat ggccgtgcgg tagcgcagga tcatgttctt ctgcatctgc
       61 gggcggcgca ggtcgatcac ccggtgggtg aggcgggtgg tctcggagag gttttcctcg
      121 tcgagctgga agggcggcgt ggcggagacg ttgaggactt cgatctcgtg acacaggact
      181 tcgatctcgc cagagacgag ctggctgttc tcggtgccgg ccggccggcg gcgcaccttg
      241 ccggtgattt tgaggacgaa ctcgttgcgc accgactcgg ccacctggaa catctccggg
      301 cggtccggat cgcacaccac ctggaccagg ccttcccggt cgcgcaggtc gatgaagatc
      361 acgccgccgt ggtcgcgacg acggtggacc cagccgcaga gggtgacgat ctggtcgagg
      421 taggcggcgg agacaaggcc gcagtattga gttcgcatgg tgggatctag gttcttgagg
      481 aaacggaacg cggctgacgg ggcagccgcg gcttacggat tctgcagggc cggcgtggcc
      541 gcgtgcgatg gcgcgggcgc ccggtggggc ggcggcgcaa cgacgcccat ggagatgatg
      601 tacttgagag cctcatccac gctcatgtcg agttcgatgg cgtcgcggcg cggcagcatc
      661 aggaagaagc ccgacgtggg gttgggcgtg gtgggcacat agacgctgac gaaatcgctg
      721 cccagatggt gggctgcgtc gcccccaggg atgccggtct ggaagccgat ggtccacatc
      781 ccttcacggg gatattggat cagcagcgcc ttgcggaacg cctgaccatt gctggaaagc
      841 agggtgtcgg agacctgctt gacgctgtaa tagatggatt tcaccaccgg gatgcgcgac
      901 agcagctttt cccagaactt caccaggtgt tggccgacga agttggctgc cgcgaggccg
      961 gtgagcagga tgagcagcag ggacaccacg acgccgatcc ccggcacgtg aaagccgatg
     1021 aaggcctccg ggcgcactga ggggggcacc accagcagga tctggtcgag ggtattgacg
     1081 atccaggcca gcaccacggc ggtgatgacc agcggaatcc agatcagcag gccggtgacg
     1141 aagtattttt tcatcgggtc aggtcggttt ccacaacctt cagtgacagg cgcaactgcc
     1201 gccacagggc gtggccggtg cgtccgcctt ggccggctcg gccttggagg cagacgaact
     1261 gccgcccttg aagtcggtgg cataccagcc attgccctta agctggaagc cgggcgccgt
     1321 gagctgcttg gcgtagctgt cggcgccaca ggacgggcag gtggtgagcg gggcgtcgct
     1381 cagtttctgg aggtggtctt tctgaaagcc gcaagcgttg cagcgatacg cgtagatggg
     1441 catgacgaac tcccggaagt caattcggtg aagtttagcg catcgcaaca tcgggcgaaa
     1501 caggcgcgca ttcaggcact tgggggtgag gggcgaagtt tcaagccccg cggggcctgc
     1561 acggggtgat cggcagacgt gccgcgcgtc agaatccgcc cagccaggcg tccgtgagcg
     1621 ggcggcccca gtacagcgcc agcaggccgg cggccgccag ataggggccg aaggggatcg
     1681 gaacctcccg gccgtgacgc cggaacacga tcagcccgac gcccaccagc gcaccgacga
     1741 cggaggagag gagaatcgtc aggggaagga tctgccaccc gaaccaggcg ccgatggccg
     1801 ccagcagctt gaaatcgccg tagcccatgc cttccttgcc ggtggtgagc ttgaacagcc
     1861 aatagaccga ccacagggtg agatagcctg ccgccgcgcc gaccactgca tcgggcagcg
     1921 ttgcaaatgc ttgctgaagg ttgagtaaca gccccagcca cagcaacggg agggtgatgc
     1981 tgtcgggcag gagctgcgtg tccagatcga tgaaggtcag ggcgatcatg gcccacacga
     2041 aggccagggc gcccagcccg gctgcgccga aaccgaaatg ccaggcggtg atcccggaca
     2101 gcatggcaca gatcacttcc accaacggat acctcaggct gatgggcgca ctgcaatgcc
     2161 ggcagcgacc ccgcaggagc aggtagctga ccaccgggat gttctcgagc gcagaaatca
     2221 gatggccgca atgggggcag cgggagcgcg gagtggccag attgaacgga tcggcgccct
     2281 cgccggcttc gcctcgcagg gccgcggctt cggcctgcca ctcccgttcc atcatgatgg
     2341 gcaggcggtg gatgacgacg ttgaggaagc tgccgacgaa cagtccgagc agcacgcaga
     2401 cggccgtgaa gaccggaggt tcggtaaaga tctgcatgga tcgagacggg cccgggcgtc
     2461 agccgcccac gacggcaccc agcttgaaaa tgggcaggta catggccacc acgagaccgc
     2521 cgatcaccac accgaggaag accatgatca tcggttcgag caactggctg aggccggcga
     2581 cggcatcgtc cacttcccgc tcgaagaatt cggcgacctt gccgagcatg gagtcgagct
     2641 ggccggattc ttcaccgatg gacaccatct gaaccaccat ggtggggaac accagggaat
     2701 tctgcatgga cacggtgagg ctggtaccgg tgctgacatc ggactggatc tgtttggtgg
     2761 cggtcttgta gacgatgttg ccggccgcac cgccgacgga gtcgagggct tccacgagcg
     2821 gtacgccggc ggcgaacatt gtggacaggg tacgggtcca gcgtgcgatg gtggccttgc
     2881 gcaccacttc gccgatcacc ggaatctgca ggatcgcccg gtctacggcg atctgcatgg
     2941 cctgcgagcg tttgtaagtg aacgagatgc cgaagacggt acccgcgagg atgccgaaga
     3001 acaggtacca atactcgacg aaaaagtcgg aaatggcgat gacgaccatt gttggcgcgg
     3061 gcagatccgc gccgaaactg gtgaagacct tcttgaactc gggcaccacg aacagcatca
     3121 tcaccgcgat gaccagcgca gctaccacga ctaccgcgat ggggtagaac atggccgact
     3181 tgatcttgga cttgatcgcc agcatctttt ccttgtaggt ggcgaggcga tcgagcagcg
     3241 agtcgaggat gccggcctgt tcgccagcag ccaccaggtt gcagaacagg gcgtcgaaat
     3301 acagaggatg tcggcggaat gcctgggaca gactgctgcc agtttcaacg tccgagcgga
     3361 cttcgttgag caggcgggca aaggacggat tgccgcaacc cttgatggcg atgtcgaaag
     3421 actggagcag gggaacgccg gagcgcatca ttgtggacag ttgccgggtg aacagggcga
     3481 tgtccttgtc ggtgatcttc ttgccacggg cgaaggcctg cttggtgatc ttgtgggcca
     3541 ggatcccctg gcggcgcaat accgtctgga ccacattggg agtggtcgcg cgcatttctc
     3601 cgcggatgat cttgccggtc ttgtccttgc cttcccagac gaagaggaat tccttggcgg
     3661 tgacgggagc gcgggaggac gtctttttgg gtacggtggc catcaaaaac tttcgcggga
     3721 ggcggtcact cgttggtgca ggcgaggact tcggtcaggg aggtgacgcc ctgcttgacc
     3781 ttgagcaacc ccgactggcg cagatctttc acgccctcga gctgggcctg ggccgcgatg
     3841 tccatggagt tgccgttcgt catgattata cgtgcgatct cttccgagat cggcatcacc
     3901 tggtaaatgc cgacccgtcc cttgtagccg gagcccttgc accgttcgca ccccttgggc
     3961 ccatagggtt gccaggtgcc gtccagatcc tgttcggtga atcccgcggt gagcagggct
     4021 tcgatcggaa tgtccatcgg ctgcttgcac gagcacagac gccgggccag gcgctgggcg
     4081 gtgatcagga tcactgacga cgcaatgtta aacggcgcga cgcccatgtt tcttaagcgt
     4141 tccagcgtgg agggcgcatc gttggtgtgc agcgtggaaa gcaccaggtg gccggtctgg
     4201 gcggccttga tggaaatttc ggcggtttcc agatcccgga tttcgcccac catgatgaca
     4261 tccggatcct gccggaggaa ggcgcgcagg gccgacgcga aggtgaggcc ggccttttcg
     4321 ttcacgttga cctggttgat cccggggagg ttgatttccg ccgggtcttc ggcggtggag
     4381 atgttcacgc cggccttgtt gaggatgttc aggcaggtgt aaagagacac cgtcttgccg
     4441 ctgccggtgg ggccggtgac gagcaccatg ccgtaggggc gctcgacggc ggcgagcagg
     4501 gcttcgcgct gatcaggatc gtagccgagg gcgtcgatgc ccagcatggc tgacgtggga
     4561 tcgaggatgc gcatcacgat cttttcgccg tgcagggtcg gcagggtcga gacgcggaag
     4621 tcgatggcct tgttcttgga caggacgagc ttcattcgac cgtcctgggg cacccgcttt
     4681 tcggagatgt ccaggcgcga gatgaccttg atccgcgcgg cgatcttttc cttgagcacc
     4741 aacggcggct gggcgatctc ccgcaggatg ccgtcggtgc ggaaacggat gcggtagtac
     4801 ttctcgtagg gttcgaagtg gacgtcggag gcgccttcgt tgatggcgtc gatgagcagc
     4861 ttctggatga agcgcaccac cggggcgtcg tctacctccg agttaacgtc ctcgatctgg
     4921 gcttccgagg cgccttcctt gcccagcaga tccatgtcga actcgtcgcc cgtcatgtcc
     4981 ttgaggacgg tggacgcgga ttcggacagg cgctcgacca actcgacgag cttgggcgcc
     5041 tcgacgacca ccgggtccac ggcgagaccg ctctggaagc ggatttcgtc gagcacccgc
     5101 aggttcgacg gatcggccat cgcgagggtg aggcggttgc cgcggcgccc caggggaacg
     5161 gcctggtgct tggccatcag cttgcggtcg atggcgtcga gggcgaaatg agcttcatcg
     5221 aagctgccca gatccagcag cggataacca aaggtggcgg cgctgaagcg ggcgacctcg
     5281 gcggcgctga tcagtccacg cttgatggcc tcgatgatga attcgttggg attgccggag
     5341 ggcgtcgtgc aggacagggc ctcggattcc atgaggcgac cgtgctggac gagggcgcgg
     5401 gcgagcccgc tcagggcgac ttgaggcgac gcggacatgc cgggctgggg gcgtgagggg
     5461 gtgtgggtgg aattgggcgg atcatgccgc ccggcggtgg gcttgtaaag ccgcactcac
     5521 agtgttttta tccggcgctg cgcgggcgga acaggcgggc gttcttgatg cgccggtctt
     5581 cggcctggat gacttcgatg gggacgccgg caatgcgcac ggagacaccg gattcaggaa
     5641 tgtcctggag atgttccaga agcaggccat tgagggtctt gggcccgtcc accggcaggg
     5701 ccaggccgag cttgcgattg agggtgcgga tcgtctggct gccatcggcg atgacactgc
     5761 cgtccttgtt ccaggccagt ttgtcggagg cgtccggcgc gctggtggtg aatttgccga
     5821 tgagttcctc gatgatgtct tcgagggtca cgagacccag cagttcgccg tactcgtcca
     5881 ccaccaggcc gatgcgttcg cggttttcct ggaagaactg gagctgggag tagatcggcg
     5941 tgctggccgg aatgaaatac gggggcgaca gcatgccgcg gatgtccgcc gcatccaccg
     6001 ggtgttcgag ggattgggcg aggatgcgcc tgacatggag gacgcccgcg actgcgtcca
     6061 tgtgcgcgtc gaataccggg atgcgcgtgt gatagcaggt ggcgatctgg tgcaggatca
     6121 cgtcgcgggg ctgggagagg tccagggcct cgatctgatg gcggggaatc atcacgtcct
     6181 cgacggtgat gtcctccagt tcgaacagat tgaccaggat gctgcggtgc ttgggcggga
     6241 tgaaggagcc ggcttccagc acgatggtgc gcaattcctc ggtggacatg ggctgattct
     6301 cgccctcgcc gttcgattgc aggcggaaca gcctgagcag gccggcaacg aacaggttca
     6361 cgaaccagat cacgaaatac agcccgcgca gcaggatcga caggggatag ctcacgacgc
     6421 acgccatgtg atcggcgtgg ttggcaccaa tgaccttggg cgtgatctcc gagaacacga
     6481 ggatcaggaa ggtgacgccc agggtgctca cacccagggc ccattcctcg ttaccgaaga
     6541 gctggatggt gatcacgctc accagcgtgg ccgaggcggc gttgatgagg ttgttgaaca
     6601 gcaggatgat gcccagcagc ttgtcggtct ggccgagcag atcaagggcg aggcgggcgc
     6661 cgcgggaacc ctgcttggcc agcgtgcgca ggcggtagcg gttggccgcc atcatggccg
     6721 tttcggccat cgagaaaaaa ccggatagcg ccagcagggc tgccaggatg gcggactgga
     6781 cggagagggg gatgctgtcc aagcgaggac ggggagatga cgaccggccg agtattcagc
     6841 ccggatgggg ccttgtcaag cggcgcgccc gagcagcacc tcggcgacga agcggctgcc
     6901 cacgtaggcc agcatcaggg ccacgaagcc cgccagcgtc cagcgcaggg ccttgcgacc
     6961 gcgccagccg cgggtgtggc gaccgatcag gagtccgccg aagatcagcc acgacaggat
     7021 cgcgaagacg gtcttgtgat cgaagcggaa ggcacgtccg aagagcactt ccgagaacag
     7081 gacgccggac cccacagtga gggtgagcag cacgaaggcg atgccgatca gccggaacag
     7141 cagggcctcc atggtcagca gcggcggcag ggaggcgaag gcgcggctca ggcgggcgcc
     7201 gtgcaggcgc cgttcggcca ccgacatgag cagggcatgg agcgcagcca gggtgaacag
     7261 gctgtaggcc agcatggcca ccacgaagtg agcgcggaac aggggcgagt gggcgttctc
     7321 cagaacgtgc tgttccggaa agaaggccgg cagcagcgcg cacagggccg cagccggcat
     7381 ggccagggcc tgcaggccgt cgaggcgggc gtagaggctc tcgatccagt aaaagagcac
     7441 cgccagccat agcatcaggg acagggccag actgaaaccg aagaccatgc cattggaggc
     7501 gaagaaggca tgatgcagcg cgaagccgtg agcgccgagg gcggccagca gcgcgccccg
     7561 ttcccaggga agaagttcct gacgggggcc gagggcaggc tgaagccagc gggatcgcca
     7621 gaaatgcagg cccagcaggg cgtacaggga ggcggggagg agatgcagta gaatgaccgg
     7681 catcgttcaa gtttactcca aggccccggt cacgaaccat catgctggac aacctcacca
     7741 cgcgcctggc caaagtcgtc aagaccctcc ggggtcaggc ccgcctcacc gaagacaaca
     7801 tccaggatgc cctgcgcgag gtgcgcatgg ccctgctgga agccgacgtc gccctgccgg
     7861 tcgtcaagga tttcatcgcc cgggtcaagg aaaaggccgt cggcaccgag gtcatctcct
     7921 ccctcacgcc gggccaggcc cttgttggcg tggtgcaccg ggagctcacc gagctgatgg
     7981 ggggggccca tacggggctc aacctggcga cccagccgcc ggccatcatc ctcatggcag
     8041 gtctgcaggg ggcgggcaag accaccacca ccggcaagct cggcaagctg ctcgtggagc
     8101 ggatgaagaa gaaggtgctg gcggtgtcca ccgacgtcta tcgtcctgcg gccatcgccc
     8161 agttgcagac cgtcgcccag caggccggcg tcgagttctt cccgtccacg ccggaccaga
     8221 agcccgtgga catcgccctc gcagcggtgg attacgcccg ccggcactac atggacgtgc
     8281 tgctggtgga taccgccggg cggcttgcca tcgaccaggc gatgatggac gagatcaagg
     8341 cgctgcacgc tgcggtcaat cccgtcgaga ccctcttcgt ggtggacgcg atgctgggtc
     8401 aggacgcggt caacactgcc cgcgccttca acgaggcgct gcccctcacg ggcgtggtgc
     8461 tcaccaagct ggacggcgat tcccgcggcg gcgcggcgct gtcggtacgg catgtcaccg
     8521 gaaagcccct caagttcgca ggcgtgggcg agaagctcgc cggcctggag gaattccacc
     8581 ccgagcggat ggccagccgc atcctgggca tgggcgacat cctgtccctg gtggaggacg
     8641 cccgccgtgg cgtggacgag gagaaggcca agcagtttgc ccagaagctc aagagcggca
     8701 agggtttcga tctcaacgat ttcaaggacc agatcggcca gatgcgcaag atgggcggcc
     8761 tcagcagcat gctcgacaag ctccccgccc agttcgccca agccgcgggc caattgcagg
     8821 gcggcgtgga ggaaaaggcg atccgccgca tcgagggcat catcaacgcc atgacgccgc
     8881 tggagcggac caagcccgag ctgatcaagg cctcccgcaa gcgccgcatc gccaccggtg
     8941 ccggggtgac cgtgcaggag gtcaaccgcc tcctcagcca gttcgagcag acccagaaga
     9001 tgatgaagca gttctccaag ggcggcatgc agaagatgat gcgcgccatg aagggnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgatg aagcagttct ccaagggcgg
     9181 catgcagaag atgatgcgcg ccatgaaggg cctgatgccc gggggcggac tgcgctgaga
     9241 tccggtgcgc ccgacggggc gtccacgtgc cttcccatgc ctgaaaccct ttacgggcgt
     9301 gcctacacgg cactcatgac ggtcgacccg gcggtcaagg cggcgcgggt ggccgtcctc
     9361 gtggccgact ggcaggccgg ccggctgccg gcggcggacg gtcgtgacgc ccccgtcctt
     9421 gctgtgcccg atgccggccg gcccgcacgg ccggcgctcc tgcctcacac cgacctgccc
     9481 cgtcgcaagc ccaccacgcc ggaaggccac gcggcgctcc tccacgccat cgctcacatc
     9541 gagttcaatg ccatcaactt ggccctggac tgcgtgtatc gcttccgtag cctgccggac
     9601 gagttttacg ccggctggct gcaggttgcg gcggaggaag cggatcactt cgcgcgggtg
     9661 tgcacccggc tggccgcgct ggggcatgcc tacggcgatt tcccggccca tcggggcctg
     9721 tgggacatgg cggtgaagac cgctgaggac ccccttgcac gtatggccct ggtgccgcgc
     9781 gtgttggaag cccgtggact ggacgccacg ccgcccatcg tggcggccct gcaccgcatc
     9841 ggcgacgcgg acagcatcgc cgtgctggac atcatcctgc gggacgaggt gggccacgtg
     9901 gccctggggg atcgctggtt ccgccacctg tgcgcggagc gcgggcttga gcccgaagca
     9961 acctatctgg cgctgatggc ggatttcgac gcgccgtggc cgcccgggcc cctgaacgtt
    10021 gaagcccgcc ggcgggcggg cttcagtgcc gcagagatcg tccggctgga ggccggacgt
    10081 ccgcgcggca aggtgcctca gagcgagcca taggagtgca ggccggacag gaacatgttc
    10141 acgccgacaa aggcgaaggc cgtcaccagc agtcccacca ccgcccacca ggccagcagc
    10201 gcgccgcgca ggcccttggt gaggcggatg tgcagccacg cggcgtagtt gagccacacg
    10261 atgaaggccc aggtttcctt gggatcccac tgccagtagg tgccccaggc ttccgccgcc
    10321 cacagggcgc cgaggatggt ggcaatggtg aagaaggcga aaccgatggc gatggccttg
    10381 tacatcacgt cgtccagcac ctcgggcgcc ggtagacggc tggcgagcag gccgcgccgc
    10441 accagcaggt ccgccacccc gatcatcgcg gccagcgcaa aggcgccgta gccgatgaaa
    10501 ttggccggca catggacctt catccagtaa ctctgcagcg ccgggatcag cggctggatc
    10561 tcctgcgctt cgcgggaaaa ggtgtaccag agcaggaagc tcaccgcggc cgagatcacc
    10621 agcagcacga aggcgcccat ccgccgcgtg gcgtagcggc cctcgtaata caggtagagc
    10681 agggacgtga gcagggtgaa cagcacgaag acttcgtaca ggttgctgag ggggatgtgg
    10741 cccacgtcgc ggccgatcag gtagctctcg taccagcgca cgaagagacc cgtggtgccg
    10801 aaggtggcgg aggtccaggt cagcgcggag ccggtcttct cggcgaagtc ggagcgggcg
    10861 gcgaggccca ggccgtagct tccggtggcg acgaagacca gggcgctcat ccacatgatg
    10921 gcggtctggc tggacaggaa atacttgagc aggaagatct tttcggcccg cgccagatcg
    10981 ccttcgtaaa gcccgacgct tgtcagggcg agggcggcga cggcggccag gaagaggcgg
    11041 aagggcttcc agcgccagcc cacggcgatc acggccggca ccacgagggc caggatggcc
    11101 atgtcgtaga cgtccatgaa gccaccgtac tgacgcaggg caaacatgcc gccgcccgcc
    11161 acggccaggg catacagcca gtcgccgggc tggaggcggc gcagcaggga aggagtgtcc
    11221 gggtgggcga tgtccatgcg gattacctcg gttcgatggc aggtggagcg tctgccggag
    11281 gggtgaagcg ggtggcaagg gcgtcccggt gggcggcgaa ggccgtgtcc acgtccaggg
    11341 acttgcggtt ggagctgagg gccacgaggg cctcgccaga gtgtttgacg agcacgaaga
    11401 ggcggcgttc ccggatgtag agcatggcga acacgcccag caccagcagc agggagccga
    11461 gatagacgat gggcttgccc ggtgagcggg tggcctggag gaccgtggcg cgcacttcgt
    11521 cgaagcggtc gagctggaag aggaggggcg cgccgtagtg ctggctgtcg ctggcggcgt
    11581 tgaggctgtc acgcacgaaa cgggcgtatt gcgcgtcgtt cttgaggggc ggcaggccgg
    11641 cgtcctgacg ggcgagctgc caggcttccc aggtcacccc ttccaggacc ttgatgaaga
    11701 cgccggcggc gcggtcgcgc tcgccttccg ggatggtgct ttcgatgaat ttgccgacgc
    11761 tctcgaagcc gccgcgggcg aacaggtcga gggtgcgacg ggcggtttcc tgcaggcgtt
    11821 cacgcagggc tgcgtcgtcg ccccggtcca gggcagtggc ggcaaagcgg cgggccaact
    11881 cgtcatggcg gcgggggtcg aggagcaccc ggcgcaaggc gaaccaggca tcagccttct
    11941 gatcggcgtc caccgggatg cgcatgaagc ggaagggcgc gtcgggcgtg tcgcgcatgc
    12001 cggcgagcag ataccaggca ccgtcctggg cgatgggctg catgtaggtc tgatattccc
    12061 gcgcctggcc ggtgttgtcg cgcagcttgt actggaagct cgggcctaca ttgtgaagat
    12121 ccttcggcgc cgtgctgcga gcaccgctgc ccagatgctt ctccagcgtg ccgagggtgc
    12181 cggacgccgc gcccgtgtcg ggatcggcga ggttttccac gttgaagggg cggaaggcaa
    12241 tgagctccag ggtcaggtcc aggccattgc gcttcagggg cagcgaatca ccgacggcga
    12301 cgtcaacgga ctgatccggc gtgccgtcga cgcgcaggtc gcgtgccagc aggcgcaggc
    12361 gcgagccgcc atcttcgaag ctcgcctgat agagcatgat gccgtccacc tcgacaggct
    12421 tgttgacctc gatggtcttc tcgtaggtct tgccatccgc gccggtgatg aggacgtcgc
    12481 tggcgaagcg cttgggcatc ccggaatcgt agtgttccag cgtgaacttg cgcagggaca
    12541 cggtgaaggg caggtcctgg agcaggatgc cgtccttcac gttgagcacc gcgacgttgc
    12601 tgctcttgcc ttcggggacg aagacgttgc cgcggaagct ccagttgtcc cggcccaggc
    12661 gactctcggg cccgatgtcg gcgatcagct ggttaccgct ggtggtgtgc ttgccaccca
    12721 gggccatctg gaccttgagg ggcaggttgc cgtccagcag gccgcccagg cagatgagga
    12781 ggatggccgc atgggccagg aagtagccga tgcggctcca gctcccctgc cggccggcga
    12841 acaggacgcc gtcgtcacgg acgttctcgc ggaagcggaa gccattgcgg atgaggtgct
    12901 cacgggtaaa gcgggccgcc tcgtcgaagg ggaggccgac ggccagaagg gcatgatgct
    12961 ggaaggcggc cagggaggtt tcccgcgcat gctcgcggaa gctgcgcatg tccttgagca
    13021 tcgggcgggt ctggcggacg atgcaggcgc cggtggacag gaccaggaaa ccgaggatga
    13081 gcaggaacca ggcggcgttg tagaccgcgt agagtccgag cttttcaaat accgggaacc
    13141 agaacggtcc gaactggttg aggtaattgt tatagggctc gttctgcttg atcaccgtgc
    13201 cgatgatcga ggcgatggcc aggaccgtca gcaggctgat cgcaaagcgc atcgagctca
    13261 ggagttcgta agtcttgcga agggtgctgc gctgcatggc tggggtgggc gatggaggcg
    13321 gagagcagtg tgacggccga cgagcgtgcc gaatgaaaaa ggggtggtcg aaaccacccc
    13381 tttttagcgt cggcgcgtcg ggattgcgtt gattcagcgc aagccggcgg cgaagtcgga
    13441 aacggccttg atttccggat cggtcatctt gagggcaatg gtgcgcatca tcttggccgg
    13501 atcgttggca cgctcacctg cacggaaggc cttcagctgg gcttcggtgt aatcggcaaa
    13561 ctgaccgccg atacgcggga actgggcggg caggccggca ccggcggggc cgtggcaggc
    13621 tgcacaggcg gggatgccgc gcttcaggtc gccggcacgc cagagcttct gaccgagctc
    13681 gatgctggcc ttgttcttgg ccgcctcggg cttgagggtc tggctgccga agtaggcggc
    13741 gaggccggtg atgtcgtcgt cggacagggg cgccgccatg ccgcccatga ccgggttggc
    13801 gcgttcggcg ggcttgccgt ccacactctt gaagttcttg agctgtttgg cgaggtattc
    13861 cggatgttgc cctgccagct tcggattggt gggaatctgg ctgttgccgt cggcaccgtg
    13921 gcaggcgccg cagacctgtt cggcggtcat tttggccttg gccaggtcgg gctgggcggg
    13981 agccttctct tcagcctgga ccgccgtggc ggccagaagc agcgaaagca gcagggaacg
    14041 cttgatcatg atgtcctcgg aagccaagaa tggtggtgtc tcaaacctgt tattctatag
    14101 caacttccgt tatttgcgcg atgcctgcgt tgtgcgccgc gccgtctgcc gcgagcgccc
    14161 gcgatgggcc cgcctcacct catgtccctg ttccgcaacg cccgcttcga gatttccatt
    14221 gccaagccca gcggcctgcc cccgcccaac ggtgcggagg ttgcctttgc cgggcgttcc
    14281 aacgcgggca agtcaagtgc catcaatacg ctggccaatc atacccggct ggctttcgtg
    14341 tccaagacgc ccggccgcac gcaactgatc aacttcttcc ggttgcagaa tggcgcagtt
    14401 ctggtggatc tgccgggcta cggctacgcc caggtaccgg aggcagtccg ccgccagtgg
    14461 cagcatctgc tggaaaacta cctcacgcgc cgcccgaacc tgatcggact ggtgctgatc
    14521 atggattccc gtcatcccct caccgagcgg gaccaggcga tgatcggctg gttcgccccg
    14581 agcggccgtc cgatgcatgt gctcctgacc aaatccgaca agctttcccg caacgaggct
    14641 gctgccacgc tgacggcggt acggcgcgaa cttgcgccct tgggcagcca ggtcacggtg
    14701 cagctcttct ccagcctcaa gaagacgggc atcgaggagg tggagcaggt ggtgggggcg
    14761 tggctggcgc cggcgcagga tcccgcctga atttttcatg acgagcaaca tttttcactc
    14821 ggcgtcacga cgaccgggtg agatcatcgc gcatcgctcg taagagccct cccgttcgtc
    14881 caatcatgac gtcctccatg cacctgcaac cgatcatcct ctccggcggc tccggcaccc
    14941 gcctgtggcc cctgtcgcgc gagcaatacc ccaagcagct cctcgccctg gtggggcacg
    15001 agaccatgct ccaggccacc gcgggccggc tggccggctt tgccggcgcg ctgccggtcg
    15061 aacaggcacc cctcgtcgtg tgtaacgagg aataccgatt cgtcaccgct gaacaactgc
    15121 gtgcctgcgg gcgcggcggc cgcgacatcg tcctcgaacc ggccggacgc aacaccgccc
    15181 ctgccctgac tctcgccgcc cttgccgtgg aagaggccga cccggtcctg ctggtgatgc
    15241 ccgccgacca cgtcatccgc gatctggccg gcttccagcg ggcgatcgcc accggcatcc
    15301 cggccgccct cgctggcgcc atcgtcacct tcggcatcgt tccggaccgt cccgaaaccg
    15361 gctacggcta catccgcttc ggcgccgaac gcaacgatgg cgcccgcagc attgccgcct
    15421 tcgtcgaaaa gcccgacgcc gccaccgccc agcgctacct ggacagcggc gaatacctgt
    15481 ggaacagcgg cctcttcatg atgcaggcga gcacctggct caaagccatg gcccacctgc
    15541 agccggccat ggaagccgcc tgccgcgccg cctgggcggg cggttcgcgg gatgccgatt
    15601 tcgtgcgcct ggacaaggcc gccttcctgg cctgcccgtc ggactccatc gactacgcca
    15661 tcatggaaaa gctcgctggc gccgaggggc tcggcatccc gggctgtgtc gtccccatga
    15721 gtgccggctg gtcggacgtg ggcgcctggg acgccctgtg gcagatcgcc gacaaggacg
    15781 aagccggcaa caccgcgcgg ggcgacgtcc tctttgaaaa cacccgcaac accctcgtcc
    15841 acgccgacag ccgcctggtg gcctgcgtcg ggctggaaga caccatcgtc gtcgaaaccc
    15901 ccgacgccgt gctggtggcc cacaaggaac gcacccagga cgtcaagcag atcgtcgccc
    15961 gtctcaaggc cgaccagcgc agccaggcct ctgcccaccg caagatccac cgtccgtggg
    16021 gctggtacga ctccatcgac agcggcgagc gcttccaggt caagcgcatc gtggtcaatc
    16081 ccggcgcgcg cctgtcgctg cagatgcact accaccgggc cgagcactgg atcgtcgtga
    16141 agggcactgc cgaggtgacc aacggcgaga agaccttcct gctgggggag aacgagtcga
    16201 cctacatccc cctgggccac gtgcatcgtc tggccaatcc gggcaaggtg ccgctggaga
    16261 tcatcgaggt gcagtccggc agttacaccg gcgaggacga catcgtgcgg tttgaggata
    16321 agtacgggcg cggctgaact cagcaatgac gacgcggacc ggcgtcaggt cggttccgcc
    16381 aggaagccga agcgggccga cacctcggcc cgcttcagtc gcccgatgct cacccgcgtg
    16441 tcgcgccaac ggaggtgcag gggctcggcg gagtccggat tcccttccag gccctccgcc
    16501 attgcagcca gatcccgatc gtccagcaga ccgatgccgt gctcgctgtc gagcaggaga
    16561 ctgccgtcgt cgtccagcca gcccgcctga agcgcatcga cgggcagtcc ggtatgggtt
    16621 tccaggctgc cgtcggatcg caggcgaagg acgtaaggcg tgtattccag gctgacaaag
    16681 accttttgcg gcccgttctg gaaaaaccag cggccggttt catccgcgcc gtaattgcgg
    16741 ccgatgaagg cgctcagtcc ggcgtggtgg atgatgccgg tcggaacgcg ccaggcgccc
    16801 cgccgggtca ggtgcagcca tcctcggcag tcaggcacgt ctggccactt cagaagggcc
    16861 tgacggacga tgtcatccat ggtgggcgcg gtgccggcga gggcggggac ttaccagagc
    16921 ttccaccagg cgctcttttc gcccggccgt ggctgtccgg aatagaaggc gctgttcgga
    16981 tagttcttgc gcaggacccg gtcggcgtcg tcgcgcagct cggtcagtcc catcgcgtca
    17041 taggccttga tcatcaggaa caggccttcc tccaccgagg gggattccgg aaagtccttc
    17101 accgcagcct gcgcccggtt ggccgccgcc acgtaggcgc cacgtcgcag gtagaaacgg
    17161 gcgacatgca cctcgtgggc ggccagggca ttgacgaggt agcgcatgcg cgcctgggcg
    17221 tcggcggcat agacgctgtt ggggtagcgg gtcaccagat ccttgaaggt gtcgaaggcg
    17281 tcgcgggagg ccttggtgtc ccgttccgac atgtcctggt tggcgagggc gccgagcagg
    17341 cccagatcct cgttgaagta cgccaggccc ttgaggtagt aggcgtaatc cacattggga
    17401 tggttcgggt gcagcttgat gaagcggtcg caggcggcca ccgcggaggc cggctcgccg
    17461 gacttgtaat aggcgtaggc cacctcaagc tgggactgct gggcgtagcg gccatagggg
    17521 tagcgcgcct ccagcttctc gaacatcttg atggcccggt cccaggcccc gtcggacatg
    17581 gattccttgg cttcggtgta gagtttctgg gcgctccagc cagcggtttc gtctgcctgg
    17641 tcggacaggc cgctgcagcc ggccagcagg agaacgccga taaacgctaa actacgcagg
    17701 gtgaagtgcg ccattgatat tccgcaggaa gatgactggc tcgattatag cccagccgat
    17761 cccgcccctt ctccgcaacg cgaaaccgtt cccgccgact gtgccggcct gcgcgccgat
    17821 caagctttgg cgcgcctgtt tcccgagcat tcccgcagcc gtctgcaggc ctggctgaag
    17881 tccggccgaa tccgcatcga cggcgcctca cccgacgcca agtgcaaggt gtggggcggc
    17941 gagacggtgg aggtgagcgc cgaacccacc gaggaaagcc tggcccagca ggccgaggac
    18001 atcccccttg cggtggtttt tgaggatgac accctcgccg tcatcgacaa gcctgtcgga
    18061 ctggtggtgc atccgggcag cggcaactgg tccggcacct tgctcaacgg gctgctgcat
    18121 cacgatccgg cattccgcgc cctgccccgg gcgggcatcg tccatcgtct cgacaaggac
    18181 accagcggcc tgctggtggt ggccaaaacg ctggaggccc agaccgacct ggtccgccag
    18241 ctccaggccc gcagcgtcaa gcgccattac tatgccctcg tacacggcca acccgcggag
    18301 cagggtgcgg tggaggcgcc catcggtcgt catcccgtgc agcgaaccaa gatggcggtc
    18361 gtgcgcggtg gccgcgaagc cctcacgcgt tatcgtgtgt gcgaacgctt tcgtcacgcg
    18421 acgctggtgg aatgcgaact cgccaccggg cgcacccacc agatccgtgt tcatatggcc
    18481 cacatcggcc acccactggt gggggatgcc acctacggca aggcccgcag cgccgatccg
    18541 cgactcgatg cctttccgcg ccaggccctg catgcctggc agctgggcct gatccatccc
    18601 cgcagtggtg aggcctgtgt ctggcagcgc gccctgccag cggatttcga aaccctgctg
    18661 gccaccttgc ggagcgtcta aggatgtcca acggcaactg gatcgttccg gactggcctg
    18721 ccccggcctg cgtccgggcc ctgtccacca cccgcctggg gggcgtgagc cctgcgccct
    18781 gggacagtct gaatctcggc acccacgtgc aggatgatcc cgcccgcgtg cttgccaatc
    18841 gggcgatcct ccgtgcgcag ctgccggccg acccggtctg gctcaatcag gtgcatggca
    18901 cgaccgtggt ggatgcggcg gtcgtgtctg gcattccgga cgccgatgcc gcgatcgccc
    18961 gcgcgtcggg cgccgtgtgc gtcgtcatga ctgccgactg tctgcccgtt ttgctctgcg
    19021 accgggccgg gacggtcgtc ggcgcggccc atgcgggctg gcgcgggctg cacggcgggg
    19081 tgatcgaggc aacgctggcc gccatgggcg tggcgccaca cgaggtgctc gcctggctcg
    19141 gcccggccat cggccctgcg gccttcgagg tgggcgacga ggtgcgggcg gccttcgtcg
    19201 ccgacgatga ggcggcggcg tccgcttttc tgccgggagc tgcgcccgac aagtggctgg
    19261 cggatctcta tcggctggcg tgtcaacgtc tggcgcgggc aggcgtcacg cgtgtgtccg
    19321 gcggcgggct gtgtaccgtg gcggatcgcc ggcggttctt ctcctatcgc cgggacggcc
    19381 tcaccggacg tatggcctcg ctggtgtggc tggccgcgcc ggacgcctga gggtatatac
    19441 tcgccggcct ttttgtgtat ccgaattcca tgacgcccct ggcctggatt gtcctgatga
    19501 gtgtcggcgg cggcgccctg agcgttctgg ctgcgtccgt cgtggcctat gcggcgcggg
    19561 agtcctgggt tccggtgctg atcagctacg ccatcggcgc gctgctcggc gcggcttttc
    19621 tcgacgtgct gccccatgcc atcgagtcgg ccgccacgcc tacacgggcg gcggcgacgg
    19681 tcctggcggg catcctgctt ttctttgtgc tggaaaaact ggtcctgtgg cgccactgcc
    19741 atgacgaggg gtgtcatggc catgggcatg accaccatca tgggcacgaa ggtcatgatc
    19801 acggtcgcag tgggctgctg atcctcgtcg gcgacacctt ccacaacttt gtagacggcg
    19861 tgctgattgc cgcggcattt gtcgacagca cggccctggg catcgtgacc gcgctcgcga
    19921 tcatcgccca tgaaatcccg caggaagtgg gggatttcct gatcctgctc cactcgggct
    19981 tcagccggac caaggcgctg gccttcaatc tgctgtcgag tctggcgacg ctggtggggg
    20041 gcttcctggc gtatttcgcc ctgtcgcaga tcgaatcgct ggttccggta tttctcgcgc
    20101 tcgcggcggc gagtatgatc tacgttgcgg tggccgacct cattcccggc ttgcacaaaa
    20161 ggacggaatt gcgcgccacc gcccagcagg tatcattgat tgctgccggt atcgccacga
    20221 tcgccatcgc ccatcccctc atcgagggcg cgctgggctg aacggcgacg gcgggctggt
    20281 gtgccgaaga gccacaagaa cagggagagc ggcgcaagac catagaagaa gaacgtcagg
    20341 agacctccga cgacggtggt ctccgctgca gtggcaagca gcgcgacata cagccaggca
    20401 atcgcaacga tatacatacc cggattctac ttggccgcgt acgtctcgcg ggggttcgtg
    20461 cgtccgcggg ggcgacacga cgctcccggg cgatgcggcg ttgggtcggg agccgggccg
    20521 caaggataaa aacatcagga gaggtgcatg acaccggagc aggcaacaga ggcattgcag
    20581 ggcatgttca agttcggcca gtccttggcg cagaattatt tcgatttcct tggcaagcag
    20641 agctccgcca tgcagcaggc ggctccggtg ggagcacccg tgccggccgt tccgctgcct
    20701 gaagcgcagg aactggcgcg actccagcag gaactcgccg aatcccacgc ccgcctctgg
    20761 agcacgctgg cgcagacccg tccgggtgag aaagccgaag ccgtggccca tcccgaaccg
    20821 ggcgatcgtc gcttcagcgc accggaatgg acctccagtc cgatcttcga ctatatccgt
    20881 cagtcctacc tgctcaactc ctccttcctg aacaaggtgg ccgatgcgct gccggtggcg
    20941 gacggtcggg ccaaggcacg catgcagttc ctgacgcgtc agtacatcga cgccctggcg
    21001 ccgtccaact tcgctgcgac caatccggag ttcatccaga ccgccctcga tacccacggc
    21061 gagagcatca cccgcggcat caagaatctc attggcgacc tggaaaaggg ccgcatctcg
    21121 atgaccgatg acgccgcctt cgaaatcggc cgcaacctgg ccacgacgcc cggctcggtg
    21181 atctacgaaa acgagctgat gcagctcatc cagtacgcgc cgctgacgga gcaggtttac
    21241 cagcgccccc tgttgatcat cccgccctgc atcaacaagt tctacatcat ggacctgcaa
    21301 ccggcgaatt cgctggtgcg gttcatcgtt gaacagggct tcaccgtgtt tctcgtgtcc
    21361 tggaggaacg ccaaggccga gcaggcgcat cacacctggg atgattacct cgagaaaggg
    21421 ccgattaccg ccctccaggt ggtgcgcgag attacccagg tcgacaagcc caacgtgctg
    21481 ggattctgcg tcggcggtac gctgctgtcg tcgtccctgg cggtcctgcg cggacgcgga
    21541 gaagacccgg ccgagagcct gaccctcatg accacgctgc tggatttcgg cgacgcgggc
    21601 gaactgggct gcctggtgga cgagacctcc gtggcggccc gcgaggcggc gatcggcaag
    21661 ggcggactgc tcatgggtcg cgaactggcc caggtgttct ccagcctgcg cgccaacgac
    21721 ctgatctggc agtacgtggt cggcaattac ctcaagggca agacgcccga ggccttcgac
    21781 ctgctttact ggaactccga cagtaccaac ctgccggggc ccttccttgc ctggtacctg
    21841 cgcaacatgt acctggaaaa caatctgcgc gtgccgggca agctggagat gctcggcgtc
    21901 aaggtggacc tgggtaaggt ggatttgccc gcctacctgc tcgccacgcg ggaggaccat
    21961 atcgtgccgt ggaccagcgc ctatctggtc cggcagatcc tcggcggcga aaccaccttc
    22021 gtgctggggg cgagcgggca cattgcgggg gccatcaatc cggcctccaa gaatcgtcgc
    22081 agctactgga ccagtgagtc ggctgccgcc gtgccggaag aatggctggc ggctgccacc
    22141 gagcgcaagg gcagctggtg gctgcactgg atcgaatggc tcaagacccg cagtggcgag
    22201 cagatcgctg cacgggtcaa gccgggcacc aaaaaataca aggtcatcga agccgcgccc
    22261 ggccgctatg tgaaggaaaa ggtctgagcc tgttcgttgt tcgatgcctg gcatcgaata
    22321 cgggacggac ggaccgcaca ctggcgcagc gtgaaggggg cgccgccggt ggtaactgac
    22381 ccctgcaaaa ccatataagg agggagaaac atggcacgtg tagcactggt aacgggtggt
    22441 atgggcggac tcggcgagtc catctgcatc aagctggcag cgctcgggta caaggtggcc
    22501 accacccatt ccccgggcaa caccaaggtg ggcgaatggc tgcagaccat gaacaacatg
    22561 ggctacggtt tcaaggccta tccctgtgac gttgcagact tcgactcctg caaggcctgc
    22621 atcgaggccg tcaccgcgga gctggggcct gtggatgtgc tggtgaacaa cgccggcatc
    22681 acccgcgaca tgaccttcaa gcgcatgacc aagggcgact gggatgcggt catgagcacc
    22741 aacctggaca gcgttttcaa catgaccaag caggtcatgg atggcatggt cgaacgcaag
    22801 tggggccgcg tcatcaacgt ttcgtccctg aatggcatca agggtgcttt cggacagacc
    22861 aactacgccg cagcgaaggc cggcatgcac ggcttcacca agtcgctggc gcaggaagtg
    22921 gccaagtccg gggtcaccat caacaccatt tctccgggtt acatcggcac caagatggtg
    22981 ctggcgattc cccaggaagt gctggacagc aagatcctgc cgcacattcc gatgggtcgc
    23041 ctcggtaagc cggaagaaat cgccggtctg atcgcctatc tctcctccga cgaggctgcg
    23101 ttcgtgaccg gtgccaacat ttctatcaac ggcggtcagc atatgttcta atggccgctt
    23161 tctttggcac tgcaactgca ataaaaggaa agtgaattga ctcaacgcat cgctcttgtt
    23221 actggcgcgg tcggtggcct cggcaccgcg atctgcaagt ccctcgctgc tgaaggcatc
    23281 cgtgtcgtcg gcaacttctt ccccggtgat cccgccaaag acgcctgggt cgaagcccgc
    23341 acggctgaag gtctggacat ccccctgtac ggtttcgacg tgtctgacta cgagcagtgc
    23401 aaggcggccg ttgagaagat cgttgccgaa gtcggcccga tcgacatcct ggtgaacaac
    23461 gccggcatca cccgcgacaa gttcttcccg aagatggaaa agggccagtg ggatgcggtc
    23521 attgcgacca acctcaccag cctgttcaac gtgacccaca ccatctcccc caagatggcg
    23581 gagcgtggct ggggccgcat cgtgaacatc tcctcggtga acggcgtcaa gggtcaggcc
    23641 ggtcagacca actactccgc cgccaaggcg ggcgtgatcg gcttcaccaa ggcgctggcg
    23701 gccgagctgg ccaccaaggg cgtgacgtgt aacgccatcg ctccgggcta catcggtacc
    23761 gacatggtca tggcgatcaa ggaagagatc cgtcagggca tcatcgacac cgtgcccgtc
    23821 aagcgtctgg gcaagccgga agagatcggc gctgcggtgg tttatctgtc ctccgaactg
    23881 gctggctacg tcaccggcgc caccctgaac gtcaacggcg gcctgtatta tcaataagct
    23941 gccgcgaaac gcgtcactga gccccgccag tccggcgggg cttttttttg cgcagcgcag
    24001 tataatcatc acaggcgaca tgatcgccag tgaggagata aatacatggc cgatcaagtc
    24061 cgcctgatca agaaataccc gaaccgccgc ctttacgata cccagaccag ttcctacatc
    24121 acgctcgccg acgtgaagca gctcgtgctc gagcatggct ccttccaggt ggtcgatgca
    24181 aagaccggcg aggaactaac ccgcaacatc cttttgcaga tcatcctcga agaggaagcg
    24241 ggcggtgcgc cgatgttcac cagcgacctg ctttcgcaga tgatccgctt ctacgggaat
    24301 gccatgcagg ggatgatggg caagtacctg gaaaacaaca tccgggcctt taccgacatg
    24361 caggccaagc tgcaggatca ggttcgctcg gtgtatggcg acaacgccac cgtcagccat
    24421 gacctgtggg cccagtttct caacctccag ggccccgcca tgcagagtat gatggctgcc
    24481 tacatggagc agagcaagcg gatgtatgcc cagatgcagg agcaactgga cagccagacc
    24541 cgtaatctgt tctctggttt ccagttcccc agtttctccg gcggctttgc cccgggcaag
    24601 gccgacgccg acgaggctgt tgcgccgcca cccgccgcgt ccaagaaatg atctccgggc
    24661 cgcagcctgc gctgcggccg gtgccttttc ccattcgcac gcatgaccca atccaagcaa
    24721 gcgggagttc cccgcgtcgg cttcgtgtct ctcgggtgcc ccaaggccct ggtggactct
    24781 gagcacatcc tcacccgcct gcgcgccgaa ggctacgaga tttcaccgtc ctacgatggc
    24841 gccgatctgg tggtcatcaa tacctgcggc ttcatcgatg cggcggtgga ggaatccctc
    24901 gacgccatcg gcgaagccct caacgagaac ggccgggtca tcgtcaccgg ctgtcttggc
    24961 gccaaggaag acgtggtgat gggcgcacac ccccaggtgc tggccgtcac cgggccccac
    25021 gccaccgaag aggtcatgca ggcggtgcac aagcacctgc ccaaacgaca cgatcccttc
    25081 gtcgatctgg tgccgcctca gggtatcaag ctgacgcccg atcattacgc ctacctgaag
    25141 atttccgaag gctgcaatca tcgctgcacc ttctgcatca tcccgtccct gcgcggcgat
    25201 ctgctcagcc ggcccatcgg cgacgtgatg cgcgaggccg agaacctggt caacgccggc
    25261 gtcaaggagc ttctggtggt gtcccaggac accagcgcct atggcgtgga cgtgaagtac
    25321 cgcactggtt tctggggtgg gcggccgctg cgtaccaagc tctacgacct gtgcaaggcg
    25381 ctcggtgatc tgggcgtgtg ggtgcgtctg cattatgtct atccttaccc gagtgtggat
    25441 gacattctgc cgctgatggc cgaaggcaag atcctgccct acctggatgt gcccttccag
    25501 catgccagcc cgcgcatcct caaggccatg aagcggcccg ccagtgcaga gaacaatctg
    25561 gagcgggtcc gccgctggcg tgagatctgc ccggacctca cgatccgatc caccttcatc
    25621 accggctttc ccggcgagac cgacgccgat ttcgaagcgc tgctccagtt cctcgaagaa
    25681 gcgcagctcg accgcgtcgg tgcctttgcg tattcaccgg tggaaggcgc ggccgccaac
    25741 gagctgcccg gcgccgtgcc ggaagaactc cgcgaagagc ggcgcatcca gctcatggaa
    25801 ttccaggagg acatctccac ggcgcgcctc gagcgcttca tcggccgcga gatgaccgtg
    25861 ctggtggacg agatcgacga ggagggcgcc ctggcccgat cgcccggcga cgcaccggag
    25921 atcgacggcc tggtgacgat cccggcgggc gagcacctgc aggtcggcga gttcgcccgc
    25981 gtgcgcatca ccgactgcga cgtgcacgac ctctacgccg aacccctcga gtagccgagc
    26041 ggctccatgg cccgccgccc gccccgcatc ggcattgccc tcggcagcgg cgcggcgagg
    26101 ggctgggccc acgtgggcgt actgcgcgcc ctggcgcggg aaggcatcga gcccgcggtg
    26161 atctgtggca gttccatcgg tgcctttgtc ggcggaatcg ccgcggcggg cgatctggac
    26221 aagcttgccg actgggtgga cagtctcaac tggcagcagg tggtcggact gctcgacatg
    26281 ggtctgcggg gcgggctcat caagggcgac aagctcatcg acttcttcca gcgccacttt
    26341 gttgaccacg acttcgcttc gctgcggcgc cctttcggct gtgtcgccac ggatctgcag
    26401 acggggcacg aagtgtggtt gcgggacggc agcgtggcga ccgccgtgcg cgcctccatc
    26461 gccatgcctg gcctgatgac gcccgtcgta cacgaaggcc ggctgctggt ggatggcggc
    26521 gtcgtcaatc ccgtgccggt gtccctcgcc cgtgccatgg gcgccgatct cgtcattgcc
    26581 gtcgatctca acgccgatgt ggtcggtcgc gcctggcgtg cgccaaacga accgcctccg
    26641 cccccgccag aagccgggcc gttctgggcg cgcaagctgc tgacccgctt tggcctgggc
    26701 gagcgacctg cgggcggcgc cggcgacgac gagggtatcg acatgccgtc catgctgtcg
    26761 gtcatccagg gcagcatctc catcatgtcc gtgcgcattg cccgcagccg gctggcgggc
    26821 gaaccggccg acgtgctcat cgcaccgcgc atggcccagg taggctggat ggagttccat
    26881 cgcggtcggg aaggcatcgc cgagggcgaa gccgccgtcg aactggcgct gccggccttg
    26941 cgtcgcgccc tggagtcctt ctagcatcca cttcatggat gcctttctcg atgcggtccg
    27001 ggccattgtc ggtccgaccc actgcctgtg cggcgaagcg gccgctccgt atctgaccga
    27061 ctggcgtggc cgttataccg gctccgcacg cgcggtggcc tttccggccg atactgccga
    27121 agtcgctgcg ctggtgcgcg tctgcgtgga tgccgacgtg cccatcgtgc cccagggcgg
    27181 caacaccggc ctgtgcggcg gcgcgactcc cctggatgac gggcgtgcgc tgctcatcca
    27241 actgtcgcgc ctgaatcgca ttcgccacgt ggatggcggc aacaatgccc tcaccgctga
    27301 ggcaggggtg acgctcgcag ccgtacatgc ggccgccgaa gcggcggatc ggcaatttcc
    27361 cctgtccttg gccagcgagg gatcctgcga ggtgggcggc gtgatttcca ccaatgccgg
    27421 cggggtgcag gtgctgcgtt acgggaacac ccgggaactg gtcctgggcc tggaagtggt
    27481 cctgcccgac ggccagatct ggaacggcct gcgcgcactg cgcaaggaca ataccggcta
    27541 cgatctgaag cacttgttca tcggcgccga aggcacgctg ggcattgtca cggccgccac
    27601 cctgaagctg tttcctcgcc cggtcgcaac ccggacggtg tggcttgcga ttccccatcc
    27661 gatggccgcc gtggccttgc tcgggcagat gcgcgcccgt gccggtgacc ggctgacggc
    27721 cttcgagctg gtgagccgcg tgagtctcga tctggtgctg cagcacatgc ccggcgcgcg
    27781 ggatcccttg ccgacggttc acccctggta cgtgctggcg gaactgaccg acaccgtccg
    27841 ggacgcctct ctggatgcca tggcggaaac gctgacggcc gaggtgctgg aatcgggcct
    27901 cgtgcaggat ggtacggtcg ccagcagcga agcacaggcg cgcgcactgt gggcgctacg
    27961 cgaacacatt tcggaagccc agaagcgtga gggctacagc atcaagcacg atatttcatt
    28021 gccggtgagc gccatccccg actttctcga tgacgtggcc ggtcgcctcg aacaggtgtg
    28081 gccgggcctg cgcatcgtgt gcttcgggca cctgggagac ggaaatctgc attacaacct
    28141 gtctctgcct gtgtcggcgg acaatgccgg gctgattgcc cggagttcgg acgtcagtgg
    28201 cctcgtccat gatcgggtgc atcgattcgg tggatcaatt tcggctgagc atggcatcgg
    28261 tcagctcaag cgaggcctgc tgccccgcta caaatcagag gtggaactgg atctgatgcg
    28321 ccgcatcaag gctgccatgg acccagccgg gcggatgaat ccggggaaag tgctgtgagg
    28381 tcaccgtggc cagcgcttcc tgtggggaat cgcggtgtga acgatctcac gggacggcgg
    28441 gaggggtctg tgtcggagaa ttttacattc ctgacagatc tgccagcttc gcagttcatt
    28501 ttagcaatgg ataacatggg cttatctgac ctggcgtgat gcttgctttt aatctgtgtg
    28561 tagagtactg cgtgaagcgc ttcacggcct tcccgggtgg taattggatt cggttcaact
    28621 tgaaataagg atgagttaca tggccaaaac aaaacagctt gccgtcgccc tgggcgcggc
    28681 cctgaccatc ggcggcgcct ccgtggccca cgctgatgtc tatgagtatg ccaacaagtt
    28741 cagcgcgaac tacgtgttct cgggtttctc cgacgcgggc actgtgggcg atgatcacac
    28801 ctttaccctg tcgttcagca acgtcagcgg caacctggat gccaccatcc ccgcctcgtc
    28861 cggttactac tccatttccc gcaagggcga gttcgacttc gactataacg gcacctctgt
    28921 tccgtccacc cttcaggcgc cttccgtgtg tagcggtttg ggcaaggcca actgggattt
    28981 ttgctccaag accactaact acgtgccggt cggtgcgggc aacttcaact ggtccgggat
    29041 cactggaacc catttcctct acaactggga caccgacaca ttcacgtcca acggcattcc
    29101 ccagaccacg ggtgacttcg ccggcaactc cacggccatc atgctgctgc tgggcacctt
    29161 cctcggcccc gtcggcacga ccatcgccac ctatgtgggc gatggcaacg tgttcgtgca
    29221 tcaggtcatg aacggcgctg cgaatacctg gacgctgact ttcaccgaag atcccggcgt
    29281 tggcatcgaa tccgtgctaa aggcgctgga tgccggcacc attccgggcc tcccgaaggc
    29341 gctgacgaac gccatgatcg atggcgtcgt gtacgccgac ggtagtctgc gcgtcgttcc
    29401 ggagccgggc agcctggcgc tgctgggtct gggtgcggct gcactggcag tgcgtcgccg
    29461 caagagcgcc tgagcggttc tgtctcggca aaacggcggc ttcggccgcc gtttttgttt
    29521 tgtgcgccct acatgggggc actcctgtgg gtgcaagtcc cgccgtaagt tgatcacagc
    29581 gaacgaagag aagcgcaact gcgtgagggc ggaccgagcg tggggaggac gcgtggattc
    29641 tggtacgggt caggcggaca ggtacggtgc aaggtggccg acaaagccaa agaggccttc
    29701 aagcaacgca tccgggagat gacgagacgc tcgggcgggc gcagcctgcc agagatcgtc
    29761 gaaagactgc gggcttacct gccgggctgg aaggcgtact tccagcttgc acagatgccc
    29821 ggggtgtttc gcgaactcga cgagtggatc cgccatcgac tgcgtgcggt gcagctcaag
    29881 cactggcgtc ggggcacgac gatgtatcgg gaattgaagg cgatgggcgc ctcggaaacg
    29941 gacgctcggc aggtggctgc gaacagtcgg agggggtggc gtaacagccg cttcctactg
    30001 aaccgtgcca tgcctgtcgc ctactttgat cggctcggtg tacctcgact ctcatgacct
    30061 caactgctcg aaccgcccgg tgcggacccg catgccgggt gctgtgggag gggaacggtc
    30121 gggtgatctg accgccccta tcccgattgg ccgcgccgca gagaagggct tcaggcaggt
    30181 acgcatgaga ggcggacggc ttccacaaaa aatttttcat agaagcttgc tttcaatatt
    30241 atttgaatct ataattctca aatcgtttcg ttgcttcaaa aatattttga gcggcggtgc
    30301 tttacaggct gtgcaatgtt tcttataatg cgcagctcgt tgattggggg tatagctcag
    30361 ctgggagagc gcttgcatgg catgcaagag gtcagcggt
//
LOCUS       DAOQMM010000036        29678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_435_length_29678_cov_43.532694, whole genome shotgun sequence.
ACCESSION   DAOQMM010000036 DAOQMM010000000
VERSION     DAOQMM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29678
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_29678_cov_43.532694"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..588
                     /locus_tag="PLH85_08075"
     CDS             118..588
                     /locus_tag="PLH85_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64558.1"
                     /translation="MPVLVENSALIELKPAKATGLGMTQLGVPVLDGTQVKKGTLHEF
                     LQSTGEGTVGRRYQNIRVTAVRTTEGGVESAKVFVAFEVFGDDNVPEGANGGFAGALL
                     KGDVVLLALPVTPLFLPYGRAWYENQAVFDVPLAVFDGAERLQFTARADSIRAI"
     gene            complement(599..1195)
                     /locus_tag="PLH85_08080"
     CDS             complement(599..1195)
                     /locus_tag="PLH85_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64559.1"
                     /translation="MPPPVPAMLTDADARARLATHPLPDNPWVVATDHAILIKADYTD
                     TIERLLRWAPRARWSARQRCWIVPLGSLELVRSILPELSRLAEAMQPQPMAAEANGSS
                     ALPADAIARDAAARRLFRDHARCLYGSDWQRETARALGRDEAALAGWLIGQGVMEAPP
                     EVLLAEMLALMRRRVTEIGAAADALERQLIASGIAPLS"
     gene            complement(1305..2495)
                     /locus_tag="PLH85_08085"
     CDS             complement(1305..2495)
                     /locus_tag="PLH85_08085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB64560.1"
                     /translation="MHGPTLLLLTCILLGMMTLALAAARSGNRHIPGLGAWTLSYLCG
                     FLGSLSFLFRPPEVSVTSVLLAQGLLFTLAYLNLAGARAYMGRRPFPAWSVGAMLVLL
                     LGVAAFFTTAVPNTPLRVGVLGAVCGALFLASAFTMARGTPQLYPARYFLALACAVHG
                     LILIVRPWLFEAGGALGVRVDGSFLLSEYALLESIVALVMMAFGVLMAAHAHTTEALR
                     QQAERDPLTGVYNSRAFAVLLDKAAHLAGRMGTPLTLLVVDLDNFKRINDTWGHRSGD
                     LALRHFVNIATRSLRTEDIMGRIGGEEFAICLPNTLPEHALSVAERLRADVVAHPALG
                     EHGTLAFTVSIGLARCSPDESPAAALHRADQAMYRAKQRGRNRVETLPASEPGPLEAM
                     PARS"
     gene            2692..3612
                     /locus_tag="PLH85_08090"
     CDS             2692..3612
                     /locus_tag="PLH85_08090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB64561.1"
                     /translation="MHALLAAIASAPRLTDAGRLFHGRGSLHPGCEHWTLDVYPPVWL
                     TTSFQPVDDAALAALGDALSARWAQLAPGEPLCWVHQTRDEVRADTRTMAGQVPAPHV
                     VSEAGARFYVHLPRGFNHGFFLDMAEGRRWVREHVAARPGARVLNLFAYTCAFSVVAL
                     QAGAGQVANVDMAGGALAIGQRNHLLNGLNGGASFLPHDIFASWGRLRRGGPYDLIVV
                     DPPSYQKGSFVAEKDYPRLMRRLPELLAPGGHALLCLNSPKLGVAFLQDGMAEQAPTL
                     GFVGRLPNPPAFADVSDERALKVLVYRAPD"
     gene            complement(3637..4290)
                     /gene="nfsB"
                     /locus_tag="PLH85_08095"
     CDS             complement(3637..4290)
                     /gene="nfsB"
                     /locus_tag="PLH85_08095"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HNB64562.1"
                     /translation="MSLTRNVYTRYTTKAFDPARRIPPEVFAELETLLRFAPSSVNSQ
                     PWHFFIADDDAGKARIARATPAAYAYNEGKIRNASHVVVLAVRKDLDDAHLAALLEQE
                     DRDGRFPTREARANQEKARAFYVGLHRNELKDVQTWASKQVYLALGALLLGAATLELD
                     AAPLEGFDTQILDAELGLAERGLTSLVIVALGYHSGDDFNARLPKSRLPAERVITRL"
     gene            4413..5324
                     /locus_tag="PLH85_08100"
     CDS             4413..5324
                     /locus_tag="PLH85_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB64563.1"
                     /translation="MTHLRDLEVLVQAAERGSLSAAARRLEITPAAASMALRRLEDAL
                     GVPLFLRSTRSLRLTEAGQIYLEHCRQALQLLADGRDALLTGQAALRGTLHLSLPSDL
                     GRNAVQAWLDDFLAAHPQLNLRLLLSDRLADIYRQPVDLALRYGSLPDSALIALPVAP
                     DNQRLLCAAPEWLARHGTPRHPQDLAGHECLCFLLGDAVHDHWRFFRDGEVQRVQVSG
                     PRVADDGDAVRRWAVAGRGIAYKSALDVADDLAAGRLVRLLPDWRGEPAPLHLLCADR
                     RQISPLVQALRAFLAERCRQRLASPGP"
     gene            5445..6779
                     /locus_tag="PLH85_08105"
     CDS             5445..6779
                     /locus_tag="PLH85_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNB64564.1"
                     /translation="METKRRFYHSLYFQVLTAIAIGVALGHFQPDTGAAMKPLGDGFI
                     KLIKMIIAPIIFCTVVVGIAGMEDMKKVGKTGGLALLYFEIVSTIALIVGLVIVNVIQ
                     PGAGMNVDPNTLDTKGIAAFTGPGKMQTTTEFLLGVIPSSVVDAFAKGDILQVLLFSV
                     FFGFALHKFGGRGTLVFDFIEKMSHVFFQIVNMIMKAAPIGAFGAMAFTIGKHGVGAL
                     AQLGQLMATFYLTCLVFIFCVLGLIAKFHGFSIWKFIKYIKEELFIVLGTSSSESVLP
                     RMMAKMENLGVKKTVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQATNTPMDLTQQITL
                     LAVLLLTSKGAAGVTGSGFIVLAATLSAVGGVPVAGLALILGIDRFMSEARALTNLIG
                     NGVATVVVGKWCKELDTNALNRHLNGETAQEADAPELVLDIQETRLPGSKAA"
     gene            6978..7943
                     /locus_tag="PLH85_08110"
     CDS             6978..7943
                     /locus_tag="PLH85_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNB64565.1"
                     /translation="MRFPPRRPLLLWLVPLLLTSTDALAWGLYTHVYFAQLLLWAIPL
                     ADPALRRLARRHPRLVMAGACLPDVALTARSAGADRLGTSHHWPQLHALMDAARNDAE
                     RAVVLGYASHLMADIIAHNHFVPAHERLWFESDLTTHVAVEWAMDHHVGGHLFARPGP
                     LLHSEREALVPLMSRAFHTHPEQVGQALATLAQGETWLRASRLHGGLYHLARFVDRRV
                     RRRFNLYLAHTSRRLGQLDRLMEGHLPSWAAEVEPVAARARLTDAPRRLLRACLPLPE
                     DFFAGACRRHPVGEIVLPDLPQGDDAGPASALLRPPGAALLSQPL"
     gene            8231..9313
                     /locus_tag="PLH85_08115"
     CDS             8231..9313
                     /locus_tag="PLH85_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64566.1"
                     /translation="MKALPQLPLWTLVAPVLGWLLLAGTRLDLGDAYLAVLAAGLLIG
                     VLASVHHAEVVAHRVGEPFGTLVLAAAVTAIEVALIVTLMLAGGESAMALARDTVFAT
                     VMIILDGIVGLCLLVGGRRFHEQQFGLYGVNAALSTLAAFVFLTLILPNFATTVPGPY
                     YSNKQLGFVAIVSLVLYGTFLFVQTVRHRDYFLPANADHDESVHAAPPSRAVTLISAL
                     LLPVGLAAVVLIAKAISPAIEAGVARLGAPQALVGVIIAVIVLLPEGLAAVRAARLNR
                     LQTSLNLALGSALASIGLSIPTVAIVSIVNGWPVQLGLDEKSMVLLVLSMLIAGQSLG
                     TGRTTMMQGAVHLVVFAVYLFFTVFP"
     gene            9338..9808
                     /locus_tag="PLH85_08120"
     CDS             9338..9808
                     /locus_tag="PLH85_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNB64567.1"
                     /translation="MIDNPRLITADARLTAVVPLIVPRADIAQVMGPAIHEVIATLHA
                     QGHAPAGPCFSLHHCRPTEVFDFEVGFPVNAPVTPAGRVVASTLPGVRVAQTVYRGPY
                     EGLGAAWGAFMAWIAAQHLQTGPGLWECYAAGPEVSPNPADWITELNCPVSDRS"
     gene            9805..10116
                     /locus_tag="PLH85_08125"
     CDS             9805..10116
                     /locus_tag="PLH85_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64568.1"
                     /translation="MKSPRFAAALAAALLSGTAGAADLCHANCASERKDCRFTAAQTE
                     ASEKGELIHTGPHAYPPTDYASTADAANQRLEEARARRRERESGCEAAYQRCANDCTP
                     R"
     gene            complement(10153..14571)
                     /locus_tag="PLH85_08130"
     CDS             complement(10153..14571)
                     /locus_tag="PLH85_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB64569.1"
                     /translation="MAAELPLGPLGADCEALLGVVDTGLLIADPDSGQVLAVNDAWVA
                     TCGIARAAALGRRQDALGLWGDERDLQRCAAALGAHGVVRDFPAVLQVRGAARRMQLG
                     GRHVTLGGRTLEVWSFRDMTAHDEARSGLERCDERLRLLQMGGRVGVWEWHPEQGEAW
                     WSPALEAMFGFHEGAARSPADFRGRVHPDDQAVLETAAAALRGTGSFAVEFRVIRAAN
                     DVAWISAHGRAVSGLDGRSMRVVGNAVVITERKRLELEAHDLLRMRNVILDNASVGIL
                     FVQDRRIAWANRHFAAMFGHDLGALIGCSMRGFYAHPDDYRSLGEEGYATLARGEPYR
                     REVEVQGRDGRRFWARLTGNALDAAVPHDGSIWIIEDVSERRRYEQMLHASEARLRDI
                     IEGTSDAIFIKNLDGSYRLANEAAARFVGLPLEEIVGRDDTALFGAEDARAIMARDRA
                     VLAAGKVSTVEETVTTADGCVRTFLATKGPMRDETGHVIGLFGISRDITDRRRAEDAL
                     RERESQLRSISDNLPGGMIYQLEVDAATGISRLVYVSSGIERVQGFTAQEVMENPDLL
                     IEQVIEEDRPRLLAAQQAAGSGPGPFQAEVRIRRPDGEIRWLSLRSAPRQTMDGRQVW
                     DGIALDVTETREAHRALMLAERNYQMLFSEMLNGFALHEIVCDTGGRPVDYRFLDMNP
                     AFERMTGLRAAEVIGRTVREVLPGTESYWIDTYGKVALDGTPVKYENYSAELGKHFEV
                     MAFRPSPGRFACMVLDISERKQAERERVQSGRALRLLSHCNFALVKAQSERQLLTDIC
                     RLFVETGGYAMAWVGVPEDDAGRRVRPVVSWGDRHGYLDTVSVSWDEAQATGRGPCGR
                     AVRSRETQISRNIPGAPEMAPWHGASQRSGFRSSLSIPLIHGETLFGVLSFYSTEEDA
                     FGVGEVALLKEVADNVAYGIHTLQVRLERDAAEAATVAKSAFLANMSHEIRTPLNAIT
                     GMAQLIRRGGLTPLQADQMGKLESAGTHLLEIINDILDLSKIEAGKFVLEDTAVDPAA
                     LVKRVAALLEPRIDPAQVSMQVNVPSLPAGLQGDPTRLQQAVLNYASNAVKFTEAGTV
                     TLGLRVQDEDADGVLLRFEVSDTGIGIEADVLPRLFSAFEQADNSTTRKYGGTGLGLA
                     ITRKLAHLMGGDAGVNSTPGEGSTFWFTARLRKGRPTAREGAPVTTVRAEDALRREFP
                     GAVVLGVDDEPINLEIAAALIEDAGLCVETAGNGREAVALAHAGRHAAVLMDMQMPEL
                     DGLEATRRIRALPGGGRVPVIAMTANAYAEDRERCLEAGMNDFLAKPVEPDRLFEMLL
                     HWLRAGATPVSGPEDEFVWSARYSVGVDLLDRQHRALLGLCAAARQCLARPPGAGEGP
                     DLHELLADMEHYADEHFRTEEALLAAAGFPYMDAQIADHQAYRESLADFLYSASFDRL
                     KAGTVEQFLLKWWVHHILEGDAAYAGWLARDATVGT"
     gene            complement(14968..18324)
                     /locus_tag="PLH85_08135"
     CDS             complement(14968..18324)
                     /locus_tag="PLH85_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB64570.1"
                     /translation="MSAQQEDITAELLLLYELSLTVGRTLDAPATCRDFLRVLMARRN
                     LTSASVWWFEPPDGNDPAPDEAAALTLIHALPRHAVPAGTLPSGHALARWARTAQTPR
                     HIPAGTPEHGALCFGEGEAEGAIALFPLDGHGVLRLHSQVAATLGERTLGQLRAVVDK
                     LATAIEGARAYARVRREIDERRQAEARLSEALEELQGLSRAVPDILVRIDPAGRLRWA
                     NDQALAVTGLPLETLLALPATAHFDEADHARVEAAIAAVFAGGQVDLEVPLRTRLGLR
                     MYHFRGVRVQAAGGEAALLASGRDISQRVEDLRAVEESRNLLQTVLETIPSRVFWKDR
                     ASRYLGCNSLFAADAGLAGPAQVIGREDGDLAWRAAAETIRHEDCRILLSGVPIPTGD
                     RRQMTADNPRAWVRGSRLPLRDAGGRIIGVLGVYEDVTRQKQTETELQVSRERLEYAL
                     RGTNEGMWDWNTDTDEVYFSPRWSSMLGYRNDELPHRLDTWADLMEPGDRERTLQHAV
                     AYLNGRIPRFEVEYRMRHRDGHWVDILSRAVAVQDLTGGTPRARRLVGTHMDISERKA
                     LQLSLQRQAAFTRAVIDAEADGVAVFHAVTGGPGVRFTVWNPSIELLCGYSLEAMNVH
                     GPIGTVFRPRRPERARRRLAQILTGRHLTGVELALHARDGTRRIVQVVTSTVARDEDG
                     VHVLAVFHDVTARKAAEAELRRHRSHLEELVAARTADLSVAKEAAEAANRAKGTFLAN
                     MSHELRTPMNAIIGLTHMVERQSSDPAQCERLRKIATSAQHLLQLLNDVLDLSKIDAD
                     RLTLERAPFRLGTVLGAVESLVGERASAKRLGLIQRIAPQLQARELIGDALRLQQILL
                     NLAGNAIKFTTEGHVTLAASIERTAPEGLHVRFEVADTGIGIPPEARQRIFDPFEQVD
                     GSTTRQFGGTGLGLTICRRLVRLMGGDIEVTSQPGAGSTFAFTICLEQGAAGIPRPDG
                     GLRPYSGAEAEARLRNRHRAARVLLAEDDWINQEVAIELLQEVAGLEVDLAIDGAQAV
                     TLAARRAYAVILMDVQMPEMDGLEAARRIRALPAHGATPILAMTANVFEDDRRACYEA
                     GMNDFIPKPVNPDVLFATVLDWLEAPEGRM"
     gene            complement(18329..19426)
                     /locus_tag="PLH85_08140"
     CDS             complement(18329..19426)
                     /locus_tag="PLH85_08140"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNB64571.1"
                     /translation="MYLRFASPAHVAAELRQSAVAGESPLWLLCVADTELPRVPELLE
                     SCRGEGVRVCGGVFPGLIHGTRVVHNGLLALPLQGASQVAVADLSAAGPRWRTPPSGA
                     GAHSAYVLLDSLAAGIAAFLDDLFDRFGGSLRYVGAGAACRDLRRAPVVFTGDGLWAD
                     GALAVLTPEHMEVHVRHGWTRVLGPFVASRSRANSILELNWRPAAALYRQSVEALAPA
                     FAGRPVFPDLGAAFPLCIGREDSEDVIRDPMGETDDGGLAVLADVAEHSPLYIAHGTP
                     ESLVAAARQAVEACAAGPRPARYFISDCYSRALMLGAGFRHELAGVATALAALTDAVP
                     EGVLALGEIASGGTRLLEFYNKTFVIAASRP"
     gene            19631..20260
                     /locus_tag="PLH85_08145"
     CDS             19631..20260
                     /locus_tag="PLH85_08145"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNB64572.1"
                     /translation="MKPAPCIRTVAVALTGASGMAYGLRLTECLLAAGCRVWLLVSQV
                     AQVVARQELDLTLPSRPAEAQALLTARFGAAEGQLQVFGRDEWFAPPASGSNPPDAMV
                     VCPCTMGSLAAIAAGLAGTLIERAADVVIKEGRPLILVPRETPFSALHLENMLRLARM
                     GVVILPANPGFYHRPQSVEALVDFVVARILDQLRVPHSLLPRWGEDATE"
     gene            20263..20859
                     /locus_tag="PLH85_08150"
     CDS             20263..20859
                     /locus_tag="PLH85_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNB64573.1"
                     /translation="MERVELPLFPLRTVLFPGGVLPLRIFEARYLDMAARCMKAGMPF
                     GVCLIQDGQEVGEAATPHSVGTTARIVSWDMPQQGVLLVTCRGERRFRIDARQTHADR
                     LQSAQVQFLDAADAAPTPIPAGRAGVLPLLKAIVSDAGDELFPPPHAFDDAAWVVRRF
                     AEVLPIPALARQRLLELDDPLQALEIVQTYLRQHQLAS"
     gene            complement(20894..21961)
                     /gene="mutY"
                     /locus_tag="PLH85_08155"
     CDS             complement(20894..21961)
                     /gene="mutY"
                     /locus_tag="PLH85_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNB64574.1"
                     /translation="MKTHHSFAATLIAWQRIHGRHDLPWQGTRDAYRIWLSEIMLQQT
                     QVDTVLPYYGRFLARFPDLASLAAAPVEDVLALWSGLGYYARARNLHACAQAVMARHG
                     GVLPADPAQLAALPGIGRSTAAAVAAFAYGTRAAILDGNVKRVLTRVFGIDGYPGERD
                     VEQHLWALAERLLPAQDVDVYTQAQMDLGATVCTRARPACGRCPLAGECVARRDERVA
                     ELPAPRPRRAVVVREGRFAAILRDGQVLLERRPPRGIWGGLLSLPELPEDVDSAAWVG
                     QRFGLDVVRVARLPDFRHVFTHFTLHLRPERVDVAASDGAPGAGQVWLRLGALADAAL
                     PAPVRRLLERLAAEGTPTVAG"
     gene            22052..22543
                     /locus_tag="PLH85_08160"
     CDS             22052..22543
                     /locus_tag="PLH85_08160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNB64575.1"
                     /translation="MTAFKNDPAVRAFRDTLGMFPTGVTVVTARGADGAPVGLTVSSF
                     NSVSLAPPLVVWSLSGHLACVPAFENAAVYAINVLAEDQQALSQRFASRADDKFAGVA
                     CTDGTDGVPLLAGCCAVMECRPFAVHPGGDHLVFIGEVIRFERDAERKPLVFQGGAYR
                     RLA"
     gene            22548..23333
                     /locus_tag="PLH85_08165"
     CDS             22548..23333
                     /locus_tag="PLH85_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB64576.1"
                     /translation="MPAPHWIESGGGTRGLLFLHGVSGGAEAARTLLPQLLPQGWRGL
                     AWDMPGYGASAPLDPLDFDTLADAIVTLLDAAGLSRAVLVGHSLGGMIALHTAARHPA
                     RVDALVLACCTPAFGAPAGPLQQAFLARRLGPLETGATMAELAADLIPAMAGPDADPA
                     LIGAHIALMTRIPPARYAAALRALVHFDARAALPGLTMPALAVAGRHDSVSTPRVVRQ
                     MAERLPAATYAELPAGHLAPFEAPEAFARLVRDFLTRLPDRPA"
     gene            23355..23726
                     /locus_tag="PLH85_08170"
     CDS             23355..23726
                     /locus_tag="PLH85_08170"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNB64577.1"
                     /translation="MDESPFKGKTGLSRVWNAFRYSMAGLAAAYRNEDAFRQEVWLSA
                     LLIPVALVLPASAVGKALMVASVLLVVIVELINSAVEAAVDRVSLERHHLAKRAKDIG
                     SAAVFVALVNVIAVWGLVLAG"
     gene            23853..24944
                     /locus_tag="PLH85_08175"
     CDS             23853..24944
                     /locus_tag="PLH85_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB64578.1"
                     /translation="MLRPLRYLPHGIAAACALIAACAALAAEPLVLQPVQLKALGIET
                     AVAAEAAAGRGARLPARVSVPNAQLRVVSAPVEGLVESLAVAPGSTVRKGQEVARLAS
                     RAALELQRDALQADSQAALLKHSLSRDETLFREGLIPESRLQATRAAAAQAGAQAAER
                     RQALGLAGAAAGTLGSALILTAPLDGVVLEQSVQTGQRVEPAAPIFRIARLSPLWLEI
                     QAPLEVAAGLREGAPVRLADGALRGRLIAIGRAVDATSQTVLLRAEVGAGAERLRPGQ
                     VVDVELDAPAGGPRRLPAAAVVRHEGASLVFVQTGSDAHGTRFEPRPVKIVAEGGEGL
                     GVDGVAATERVVVKGASGLKALLAGVGRE"
     gene            24941..28030
                     /locus_tag="PLH85_08180"
     CDS             24941..28030
                     /locus_tag="PLH85_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB64579.1"
                     /translation="MILDALIRFALTQRLLLLVATALLAAAGVDAYLNLPIDAFPDVS
                     STQVKIILKAPGMTPEEVETRIAVPVEQEMLGIPRQRLLRSMSKYALTDITIDFEDGT
                     DIYWARQQVGERLANALGNLPPGVSGGMAPITTPLGEMFMFTVDGELPLAERRALLDW
                     VIRPQLRTIPGVADVNSLGGEVRSFEVVPRPQSMAARGLALKDLQAVLEANNRNDGAG
                     RLNDGEEGLLVRAEGAIQTLDDLRAIVVQARDGRVTRVADVAEVRWGALTRYGAVSAD
                     GRGEAVEGMVLGLRGADAKQVVAGVKARLAEIEPTLPEGVHLTVLYDRSRLVERAVGT
                     VSKALGEAIVLVVLLLLAFLGNLRAALVVALMLPLAALSTFILMRFDGLSANLMSLGG
                     LAIAIGMLVDAAVVVVENVENQLAEAQANLPTLHVIYRATREVAVPVASGIAIIIIVF
                     LPLLTLQGLEGKMFAPVALTVVFALFSSLLLSLTVVPVLASWLIRRGAHHEPWLVRVL
                     TAAYTRLLDRALARGRQFVLIAVAGLAAAAVAFTFLGKSFMPTMDEGDLILQLEKLPS
                     IGLDQSLAVDQRLQQALLARVPEIRAIVARTGSDELGLDPMGLNQTDTFLVLTPRDTW
                     RHADPAWLADRVREVMADFPGIGYSFTQPIDMRVSEMLTGVRGDVAIKVFGPDLATLN
                     RLAKAIETAVRAVPGAEDTFTLKNDGVQYLKVSVDRLAAGRFGLNVDDIQNDLKALLE
                     GRQVGVVVEHGRRLPVLLRGPDALRDSPADFAALRLSLPDGGSVPLADVARLTRIDGP
                     VKVDRENAQRYVVIQSNVRDRDLVGFVEDARAAVAAAVPLPAGYRLVWGGQFENQQRA
                     AARLMVVVPVALVLIFLLLFSTFRSVRQALLVLSNIPFALIGGVFGLLLAGEYLSVPA
                     SVGFIALLGVAVLNGVVMVSHFNQLLERGLPLAEVVREGARRRLRPVLMTASIAAFGL
                     VPMLFASGPGSEVQRPLAVVVIGGLVTSTALTLILLPVLFRRFGVAAAPTLPEAPRG"
     gene            28023..28325
                     /locus_tag="PLH85_08185"
     CDS             28023..28325
                     /locus_tag="PLH85_08185"
                     /inference="COORDINATES: protein motif:HMM:NF023014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HNB64580.1"
                     /translation="MADVLLTLIVPTDIAQHVEDLLLSRPDLVRGFTTSAADGHGSVV
                     TLSGAEELVGGHAPRMLIRTAGPEDLIRSLMAAVKEAFPRANLYYWLVPLLDRGQL"
     gene            28322..29578
                     /locus_tag="PLH85_08190"
     CDS             28322..29578
                     /locus_tag="PLH85_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB64581.1"
                     /translation="MIRPLALLAALLITAAPAGAAAPADTAILPPDAAVARVLRAQPA
                     IQAAVSQIRAEEATRRRLEAGPYEWALRLGGQQRLTRLPATPEERFTEWNAALERPLR
                     LPGKAAADAETGAAGVALAEIAHQDMRHETSRVLLAGWFTWLRARAGAEQWAAQVALL
                     ERQAAAVSRRQQLGDAARIETVQAEAALAQARAQGGQARSRAQVAAEDLRRRFPGLPV
                     DAPARVPEPAPVLGSEDDWIARQLEHNHELGVARNETRRQHALASRAGHERSPDPSVG
                     VQVARERGGEDRLVGAFVSIPLPGAARAASADAALAQADAAHRREAAVLQRITAEAAA
                     LYRNAVAAVDAWRSLHDAALRLDSAATLTARAYQLGEGSLNDVLLTRRQAHEATLAAT
                     TAQLDALELGYRLQLDAHALWDLDEE"
BASE COUNT         4163 a        11375 c         9856 g         4284 t
ORIGIN      
        1 ccccactggc accgaacctg cttccccacc tccgtcgccg cgcgggcgcg cctttcggcg
       61 tgtcgcgtgt cgcacacgcg ccctgtcctt gtcgcattcg ataccacgga gagcatcgtg
      121 ccggtccttg ttgaaaattc cgccctgatc gaactgaaac ccgccaaggc caccggcctg
      181 ggcatgaccc agctgggggt gcccgtgctc gacggcacgc aggtgaaaaa gggcacgctg
      241 catgaattcc tgcaatccac cggcgagggt acggtggggc ggcgttacca gaacatccgg
      301 gtgacggcgg tgcgaaccac cgaggggggc gtggagtcgg ccaaggtctt cgtggccttc
      361 gaggtcttcg gcgacgacaa cgtgccggaa ggcgccaacg gcggctttgc gggggcgctg
      421 ctgaagggtg acgtggtcct gctggccctg ccggtgacgc ctctgttcct gccctacggc
      481 cgcgcctggt atgagaatca ggcggtcttc gacgtgcccc tcgcggtctt cgatggggcc
      541 gaacggctgc aattcaccgc ccgggccgat tcgatccggg cgatctgaac gcccggcctc
      601 agctgagcgg cgcgatgccg ctggcgatga gctgccgttc cagcgcgtcg gcggcggcgc
      661 cgatctcggt gacgcggcga cgcatcagcg ccagcatctc ggcgagcagc acctcaggcg
      721 gcgcctccat caccccctgc ccgatcagcc agccggccag ggcggcttcg tcgcggccca
      781 gcgcccgcgc ggtctcccgc tgccagtcgc tgccgtagag acagcgtgcg tggtcgcgga
      841 agagcctgcg cgcggccgcg tcacgggcga tggcgtcggc cggcagcgcg ctgctgccgt
      901 tcgcctcggc ggccatcggt tgcggctgca tggcctcggc cagacggctc agttccggca
      961 ggatggagcg caccagttcc aggctgccca ggggcacgat ccagcagcgc tggcgggcgc
     1021 tccagcgcgc cctgggcgcc cagcgcagca ggcgttcgat ggtgtcggtg tagtcggcct
     1081 tgatgaggat ggcgtgatcg gtggccacca cccagggatt gtccggcaga ggatgggtgg
     1141 cgaggcgggc acgggcgtcg gcgtcggtga gcatggcggg cacaggcgga ggcattggca
     1201 cgctgcaagc aagttgggcg ccaggaatcc ggcggccccg ggcggtgggg ctgggtgccc
     1261 ggcccctgtc gcggcgcacc gtacgtgtcg actcgcggag cggctcagct gcgcgccggc
     1321 atcgcctcca gcggaccggg ctcgctggcg ggcagggttt ccacccggtt gcgcccccgc
     1381 tgcttggcgc ggtacatggc ctggtcggcc cggtgcaggg cggccgccgg cgactcgtcc
     1441 gggctgcagc gggccaggcc gatgctgacc gtgaaggcca gggtgccatg ttcgcccagc
     1501 gcggggtggg ccaccacgtc ggcgcgcagg cgttccgcca cgctgagggc gtgctcaggc
     1561 agcgtgttgg gcaggcagat cgcgaactcc tcgccgccga ttcgccccat gatgtcctcg
     1621 gtgcgcaggc tgcgggtggc gatattcacg aaatggcgca gggcgaggtc gccgctgcgg
     1681 tgcccccagg tgtcgttgat gcgcttgaag ttgtccagat ccaccaccag cagggtcagg
     1741 ggcgtgccca tgcggccggc cagatgggcg gccttgtcca gcaggaccgc gaacgcgcga
     1801 ctgttgtaga cgcccgtcag cggatcgcgc tcggcctgct ggcggagggc ctcggtggtg
     1861 tgggcatggg ccgccatcag aacgccaaag gccatcatga ccagggccac gatggactcc
     1921 aggagggcgt attcggacag caggaagctg ccatccaccc ggacccccag ggcgccgccc
     1981 gcctcgaaca gccacgggcg gacgatcagg atgaggccgt gcacggcaca ggccagggcc
     2041 agaaagtagc gggccgggta cagctggggc gtgccccgcg ccatcgtgaa ggcgctggcc
     2101 aggaacaggg ctccgcacac ggcgcccagc acgcccaccc gcagcggggt attgggaacc
     2161 gccgtggtga aaaacgcggc cacgcccagc aacagcacca acatggcgcc cacgctccac
     2221 gcgggaaagg gacgtcgccc catgtacgcc cgggcgccag ccaggttcag gtaggccagc
     2281 gtgaagagca agccctgcgc cagcaggacg gaggtcaccg acacctcggg tgggcggaac
     2341 agaaagctca ggctgcccag aaagccgcac agatacgaga gcgtccacgc gcccagcccc
     2401 ggaatatggc ggttgccgct gcgggccgcc gccagggcca gcgtcatcat gcccagcagg
     2461 atgcaggtca ggaggaggag cgtggggccg tgcatggggg atcgcctgag ggccggacgg
     2521 gcgtcgatgg gacagggaag ggcacgatgc gggcttcacc tgccgacgtc aagcaccgga
     2581 tgtcgcggat cgggggaaat tctgcaggcg gcccgcatcc aggcgccgtg gaccggtcga
     2641 acagcccgtg cccggtgtag gatgcgcgcc gtccttcccg tccctgcgcc catgcacgcc
     2701 ctcctcgccg ccatcgcctc cgcgccccgc ctgaccgacg ccggacggct gtttcatggc
     2761 cgcggcagcc tgcatcccgg gtgcgagcac tggacgctgg acgtctatcc ccccgtgtgg
     2821 ctgacgacca gcttccagcc ggtggacgac gccgcactgg ccgccctcgg ggacgccctg
     2881 agcgcccgct gggcgcagct ggcgccgggc gagcccctgt gctgggtgca tcagacccgc
     2941 gacgaggtgc gcgccgacac ccgcacgatg gccggccagg tgccggcgcc ccacgtggtg
     3001 agcgaggccg gcgcgcgctt ttacgtgcat ctgccgcgag gtttcaacca cggcttcttt
     3061 ctggacatgg cggaaggccg acgctgggtg cgcgagcacg tggcggcgcg gccgggcgcc
     3121 agggtgctca acctcttcgc ctacacctgc gccttctcgg tggtggccct gcaggccggc
     3181 gcgggacagg tggcaaacgt ggacatggcc ggcggcgcgc tggccatcgg gcagcgcaac
     3241 cacctgctca acggcctcaa cggcggggcg agcttcctgc cccacgacat cttcgcctcc
     3301 tggggacgcc tgcgccgcgg cggaccttat gacctgatcg tggtcgatcc acccagctac
     3361 cagaagggca gcttcgtcgc cgagaaggac tatccgcgcc tgatgcgccg tctgccggag
     3421 ctcctcgccc ccggcggcca cgccctgctg tgcctcaact cgcccaagct gggcgtggcc
     3481 ttcctgcagg atggcatggc cgaacaggcg cccacgctcg gtttcgtcgg acgcctgccc
     3541 aatccgccgg ccttcgccga cgtatcggac gagcgggcgc tgaaggtgct ggtctaccgg
     3601 gcgccggact gagcaccgcg gcgggcggcg gtgggatcag agccgcgtga tgacccgctc
     3661 cgcaggcagg cgggacttgg gcaggcgggc attgaagtca tcgccgctgt ggtagcccag
     3721 ggcgacaatg acgaggctgg tcaggccgcg ttcggccagt cccagctccg cgtcgaggat
     3781 ctgcgtgtcg aagccctcca gcggcgcggc gtcgagttcc agcgtggcgg cgcccagcag
     3841 cagcgccccg agggccaggt agacctgctt cgaggcccag gtctgcacgt ccttcaactc
     3901 gttccggtgc agtccgacgt agaaggcgcg ggccttttcc tgattggcgc gggcctcgcg
     3961 tgtggggaag cggccgtccc ggtcctcctg ctccagcagc gcggccaggt gggcgtcgtc
     4021 cagatccttg cgcaccgcca gcacgaccac gtgggaggcg ttgcggatct tgccctcgtt
     4081 gtaggcgtag gcggccggcg tggcgcgggc gatgcgcgcc ttgccggcat cgtcgtcggc
     4141 gatgaagaaa tgccagggct gggaattcac cgacgacgga gcaaagcgca gcagggtttc
     4201 cagttcggca aacacctcgg gcggaatgcg ccgggcgggg tcaaaggcct tggtggtgta
     4261 gcgggtgtag acgttgcggg tgaggctcat gacgaaatcc ttgttcgaat ggacgcgccc
     4321 atcatgcgcc gcctcgcgct ttcaaaaaac cacaatgctg ttgattcact ttcaagatat
     4381 tctgaagaat cttcccctcc ggaacgccat ccatgaccca cttgcgcgac ctggaagtgc
     4441 tggtccaggc ggccgagcgc ggcagcctgt cggccgcggc gcgccggctg gagatcaccc
     4501 cggcggcggc gagcatggcc ctcaggcgcc tggaggacgc cctcggcgtc cccctgttcc
     4561 tgcgctccac ccgcagcctg cgcctcaccg aggcgggcca gatctacctc gagcactgcc
     4621 gccaggccct ccagctgctg gccgacgggc gcgacgcgct gctcaccggg caggcggccc
     4681 tgcgcggcac cctgcacctg tccctgccct ccgacctggg ccgcaatgcg gtgcaggcct
     4741 ggctcgacga cttcctcgcc gcacatccgc agctgaacct gcgcctgctc ctcagcgacc
     4801 gcctcgccga catctatcgc cagcccgtgg acctggccct gcgctacggc agcctgccgg
     4861 attccgccct gatcgccctg ccggtggcgc ccgacaacca gcgtctgctg tgcgcggcgc
     4921 ccgaatggct ggcccgtcac ggcaccccgc gccacccgca ggatctggcc ggccacgagt
     4981 gcctgtgttt cctgctgggc gacgcggtgc atgaccactg gcgtttcttt cgcgacggcg
     5041 aggtgcagcg cgtgcaggtg tccggcccgc gggtggcgga cgatggcgac gccgtgcggc
     5101 gctgggcggt ggccggccgg ggcatcgcct acaagtcggc actggatgtg gccgacgatc
     5161 tggccgccgg gcgactggtg cgcctgctgc ccgactggcg cggcgaaccg gccccgctgc
     5221 atctgctgtg cgccgaccgg cgccagatca gcccgctggt gcaggccctg cgtgccttcc
     5281 tcgccgagcg ctgccggcag cggctggcgt cgcccggtcc ctgaaaccgc gcgaccggct
     5341 gcctgccgcg gcccgattgc ggctttccac aatggggatg gacacaattc atttccccct
     5401 ccgccgtgcc tacactggaa tcatcaaaaa ccgggggaaa gaccatggag acaaaacgcc
     5461 gcttctatca ttcgctgtac ttccaggtgc tcaccgccat cgccatcggc gtggccctgg
     5521 ggcacttcca gcccgacacc ggcgcggcca tgaagccgct cggcgacggc ttcatcaagc
     5581 tcatcaagat gatcatcgcg ccgatcatct tctgcaccgt cgtggtcggc atcgccggca
     5641 tggaagacat gaagaaggtg ggcaagaccg gcggcctcgc cctgctgtac ttcgagatcg
     5701 tctccaccat cgccctgatc gtgggtctgg tcatcgtcaa cgtgatccag ccgggcgccg
     5761 gcatgaacgt ggatcccaac acccttgaca ccaagggcat cgccgccttc accgggccgg
     5821 gcaagatgca gaccaccacc gagttcctcc tcggcgtgat cccctccagc gtcgtggatg
     5881 ccttcgccaa gggagacatc ctgcaggtgc tgctgttctc ggtgttcttc ggcttcgccc
     5941 tgcacaagtt cggcggtcgc ggcacgctgg tcttcgactt catcgagaaa atgtcccacg
     6001 tcttcttcca gatcgtgaac atgatcatga aggccgcccc catcggcgcc ttcggggcca
     6061 tggccttcac catcggcaag cacggcgtcg gcgccctcgc ccagctcggc cagctcatgg
     6121 ccaccttcta cctgacctgt ctggtcttca tcttctgcgt gctgggcctc atcgccaaat
     6181 tccacggctt ctcgatctgg aagttcatca agtacatcaa ggaagagctg ttcatcgtgc
     6241 tcggcacctc gtcgtccgaa tccgtgctgc cgcgcatgat ggccaagatg gagaacctgg
     6301 gcgtcaagaa gaccgtggtc gggctggtga tccccaccgg ctactccttc aacctggacg
     6361 gcacggcgat ctacctcacc atggccgccg tgttcatcgc ccaggccacc aacaccccga
     6421 tggacctgac ccagcagatc accctgctgg ccgtgctgct cctcacctcc aagggcgcgg
     6481 cgggcgtcac cggcagcggc ttcatcgtgc tggccgccac cctcagcgcc gtgggcggcg
     6541 tgcccgtggc cggtctggcc ctgatcctcg gcatcgaccg cttcatgtcc gaagcccgcg
     6601 ccctcaccaa cctgatcggc aacggcgtcg ccaccgtggt ggtgggcaag tggtgcaagg
     6661 aactggacac caatgccctg aaccgccacc tcaacggtga aaccgcccag gaggccgatg
     6721 cccccgagct ggtgctggac atccaggaaa cccgcctgcc cggctccaag gccgcctgaa
     6781 ccatcgctca acgatagacc gaccctgaca gcgctcggcc cggcggagac cccgtcgggc
     6841 cgagtcgctt ttggcccccc tgcgccagcg ccgggggcca ccccgccagc cgatctaaac
     6901 caaaccgcaa ccgcgacgtc acccgcctga cactccggct gcacacgcac gtcatccgcg
     6961 cccggcacag tggcgcgatg cgtttcccgc cccgtcgccc cctcctgctg tggctcgtgc
     7021 ccctgctcct caccagcacc gacgcgctgg cctggggcct ctacacccac gtgtatttcg
     7081 cccagctgct gctgtgggcg atcccgctgg ccgacccggc cctgcgtcgc ctggcccggc
     7141 gccatccgcg cctggtgatg gccggcgcct gtctgccgga cgtggccctc accgcccgca
     7201 gcgccggcgc cgaccgcctc ggcaccagcc accactggcc ccagctccac gccctgatgg
     7261 acgccgcccg gaacgatgcc gaacgcgccg tggtcctggg ctacgccagc catctgatgg
     7321 ccgacatcat cgcccacaac cacttcgtgc cagcccacga gcgcctgtgg ttcgaatccg
     7381 acctcaccac ccatgtggcc gtcgaatggg ccatggatca tcacgtgggc ggccacctct
     7441 tcgcccgccc gggcccgctg ctgcacagcg agcgggaagc gctcgtaccg ctgatgtccc
     7501 gcgccttcca cacccatccg gagcaggtcg gccaggccct ggccaccctc gcccagggcg
     7561 agacctggct gcgcgccagc cgcctccacg gcggcctcta ccacctcgcc cgttttgtcg
     7621 accggcgcgt gcggcggcgc ttcaacctct accttgccca cacctcgcgt cgcctcggcc
     7681 agctcgaccg cctgatggaa ggccacctgc cgagctgggc cgccgaggtg gaacccgtcg
     7741 ctgcccgcgc ccggctgacc gacgcaccgc gccgcctgct gcgcgcctgc ctgcccctgc
     7801 cggaagactt cttcgccggt gcgtgccgcc gccacccggt cggcgagatc gtcctgcccg
     7861 acctgccgca gggcgacgac gccggtccgg cctccgccct cctccgccca cccggagcgg
     7921 ccctcctcag ccagcccctg tagtccggcc ctgggagtcc ggcccccgcc cggcgcacgc
     7981 cacgccgtcg aggcgtaccg atccccgcgc ggttctgata ccttcccacc ctgaggccgg
     8041 acgcccccgc gatgatgcgg gaatgcccgc cctccggact ttgcggagca cccgatggat
     8101 gcccgacctg tggccggcga ggtgttgccg cccggtcggc ccgcgccatc gggtgcgacc
     8161 gcccgtatcg gtcggccgat cctgccccgc cgcaggccgg tcgtccatcg cccgcccatc
     8221 aggacacccg atgaaagccc tgccgcaact ccccctctgg accctcgtcg cccccgtgct
     8281 gggctggctg ctcctggccg gcacccgcct cgacctgggc gacgcctacc tggccgtgct
     8341 cgccgccggc ctgctgatcg gtgtgctcgc ctccgtccat cacgccgaag tggtcgccca
     8401 ccgggtcggc gagccattcg gcaccctggt gctcgccgcg gcggtcaccg ccatcgaggt
     8461 ggccctcatc gtcacgctca tgctcgccgg cggggaatcc gccatggccc tggcccgcga
     8521 cacggtgttc gccaccgtga tgatcatcct cgatggcatc gtcggcctgt gcctgctggt
     8581 gggcgggcgg cgcttccacg aacagcagtt cggcctgtac ggcgtcaacg ccgcgctgtc
     8641 caccctggcc gccttcgtct tcctgaccct gatcctgccc aatttcgcca cgaccgtacc
     8701 gggcccctac tacagcaaca agcagctcgg tttcgtggcc atcgtgtcgc tggtcctcta
     8761 cggcaccttc ctgttcgtcc agaccgtgcg ccaccgggat tacttcctgc cggccaacgc
     8821 cgaccacgat gaatccgtcc atgccgcgcc accgagccgg gccgtcaccc tcatcagcgc
     8881 cctgctgctg ccggtcggcc tggcggccgt cgtcctgatc gccaaggcca tttcacccgc
     8941 catcgaagcc ggtgtggccc gcctgggcgc cccgcaggcc ctggtggggg tgatcatcgc
     9001 ggtcatcgtg ctgctgcccg aaggcctcgc cgccgtccgc gccgcccgcc tcaaccgcct
     9061 gcagaccagc ctcaacctgg ccctcggatc ggcgctggcc tccatcggcc tgtccatccc
     9121 caccgtcgcc atcgtctcca tcgtcaacgg ctggccggtc cagctgggcc tcgacgagaa
     9181 gtccatggtg ctgctggtgc tctccatgct catcgccggg cagtccctcg gcaccgggcg
     9241 gacgacgatg atgcagggcg ccgtccacct ggtggtgttc gccgtctacc tgttcttcac
     9301 ggtctttccc tgaacgctca aacgcccgac gtccatcatg atcgacaacc cccgcctcat
     9361 caccgccgac gcccgcctca cggccgtggt gcccctcatc gttccgcgcg ccgacatcgc
     9421 ccaggtgatg ggcccggcca tccatgaagt catcgccacc ctgcacgccc agggccatgc
     9481 gccggccggg ccgtgtttct cgctgcatca ctgccgcccc accgaggtct tcgatttcga
     9541 ggtgggtttt cccgtcaacg cgcccgtgac acccgccgga cgcgtcgtcg ccagcaccct
     9601 gcccggcgtg cgcgtggccc agacggtcta ccggggtcct tatgagggcc tcggagcggc
     9661 atggggtgcc ttcatggcct ggatcgccgc ccagcacctg cagaccgggc ccgggctgtg
     9721 ggaatgttat gccgccgggc cggaagtcag ccccaacccg gcggactgga tcaccgaact
     9781 caactgcccg gtcagcgatc gatcatgaaa tccccgcgct tcgcagccgc attggcggcc
     9841 gcgctcctga gcggcaccgc cggcgctgcc gacctgtgcc acgcgaactg tgcgtccgaa
     9901 cgcaaggact gccgcttcac cgcagcccag acggaagcca gcgagaaggg cgagctgatc
     9961 cacacagggc cccatgccta cccgccgacc gattacgcgt ccaccgccga tgcggccaac
    10021 cagcgcctcg aggaagcccg cgcccgccgc cgcgagcggg agtccggctg cgaagcggcc
    10081 taccagcgct gcgccaacga ctgcacaccc cgctgaatcg gcaccgcggg ccgcccccgc
    10141 ccggcacggg gatcaggtgc ccacggtcgc gtcgcgggcg agccagcccg cgtaggccgc
    10201 gtcgccctcc aggatgtggt gcacccacca cttcaggaga aactgctcca cggtgccagc
    10261 cttgagccga tcgaaggagg ccgaatacag aaaatcggcc agcgactccc gataggcctg
    10321 gtgatccgcg atctgggcgt ccatgtaggg aaagccggcc gcggcgagga gcgcctcctc
    10381 ggtacggaag tgttcgtcgg cgtaatgctc catgtcggcc agcagctcat gcagatccgg
    10441 cccctcgccg gcacccggcg gacgggcgag gcactgccgt gccgccgcgc acaagcccag
    10501 cagggcgcgg tgctggcggt cgagcagatc gacgccgacg ctgtagcgcg ccgaccagac
    10561 gaactcgtcc tcgggtccgc tcaccggcgt cgcacccgcc cgcagccagt ggagcagcat
    10621 ctcgaagagg cggtccggct ccaccggctt ggccagaaaa tcgttcatgc ctgcttcgag
    10681 gcagcgctcc cgatcttccg catacgcatt ggcggtcatc gcaatgaccg gcacccgccc
    10741 tccgccgggc agggcgcgaa tgcgccgggt ggcctccagc ccgtccagtt cgggcatctg
    10801 catgtccatc agcaccgcgg cgtggcggcc cgcatgggcc agggcgaccg cttcgcggcc
    10861 attgcccgcc gtctccacgc acagcccggc gtcctcgatc agtgccgcgg cgatctccag
    10921 gttgatcggc tcatcgtcca cccccagcac gacggcgccc ggaaattccc gccgcagggc
    10981 atcctcggcc cgcacggtgg tgacaggcgc cccctcccgt gccgtcggcc ggcccttgcg
    11041 caggcgcgcg gtgaaccaga aggtgctgcc ctcgccgggc gtgctgttca cgccggcatc
    11101 gccccccatc aggtgcgcca gcttgcgcgt gatggccaag cccaggccgg tgccgccgta
    11161 cttgcgggtg gtgctgttgt cggcctgctc gaaggccgag aacagacggg gcagcacgtc
    11221 ggcctcgatg ccgatgcccg tgtcgctcac ctcgaagcgc agcaacaccc cgtcggcgtc
    11281 ctcgtcctgc acccgcaggc ccagcgtgac ggtgcccgcc tcggtgaact tgaccgcgtt
    11341 gctggcgtag ttgagcacgg cctgctggag gcgcgtcgga tcgccctgca ggccggccgg
    11401 cagggacggc acgttcacct gcatgctcac ctgcgccggg tcgatgcgcg gttccagcag
    11461 ggccgcaacc cgcttcacca gcgccgccgg atccaccgcg gtgtcctcca gcacgaactt
    11521 gccggcctcg atcttcgaca gatccaggat gtcgttgatg atctccagca aatgggtgcc
    11581 ggcgctctcc agcttgccca tctggtccgc ctgcaggggc gtgaggccgc cgcggcggat
    11641 cagctgggcc atgcccgtga tggcattgag gggcgtgcgg atctcatggc tcatgttggc
    11701 caggaaggca ctcttggcca ccgtggcggc ctcggcggcg tcccgctcga ggcgcacctg
    11761 cagcgtgtgg atgccgtagg ccacgttgtc ggccacctcc ttgagcaggg ccacctcgcc
    11821 caccccgaag gcgtcctcct cggtggaata gaagctcagc acaccgaaca gggtctcgcc
    11881 gtggatcagc ggaatgctca ggctggagcg gaacccggaa cgctgcgacg ccccgtgcca
    11941 cggcgccatc tcgggcgcgc ccgggatgtt ccggctgatc tgcgtctccc ggctgcgcac
    12001 cgcccgcccg cagggccccc gtccggtagc ctgggcctca tcccacgata ccgaaaccgt
    12061 gtcgagatag ccgtgccggt cgccccagga caccaccggc cgcacgcggc ggccggcatc
    12121 gtcctcgggt acgcccaccc aggccatggc gtagccgccc gtctccacga acaggcggca
    12181 gatgtccgtg agcagctgcc gttctgactg ggccttgacc agggcaaagt tgcaatgact
    12241 gagcaggcgc agggcgcgcc cggactggac ccgctcccgc tcggcctgct tgcgctcgct
    12301 gatgtcgagc accatgcagg caaaacggcc cggcgagggg cggaaggcca tcacttcgaa
    12361 gtgcttgccc agttcggcgg aatagttctc gtatttgacc ggcgtcccgt ccagggcgac
    12421 cttgccgtag gtgtcgatcc agtaggattc ggtgcccggc agcacctcgc gcacggtgcg
    12481 cccaatcacc tccgccgcgc gcaggccggt catccgctcg aaggccgggt tcatgtcgag
    12541 gaagcggtag tccacggggc ggccaccggt atcgcagacg atctcgtgaa gcgcgaagcc
    12601 gttgagcatc tcgctgaaga gcatctggta attgcgctcg gccagcatca gggcccgatg
    12661 ggcctcacgg gtctcggtga cgtccagcgc gatgccgtcc catacctgac gtccgtccat
    12721 ggtctgacgc ggcgcggagc gcagggacag ccagcggatc tcgccgtcgg ggcggcggat
    12781 gcgcacctcg gcctggaacg gtcccggccc gctgccggcg gcctgctgcg ccgccagcag
    12841 gcgcggccga tcctcctcga tgacctgctc aatgaggagg tccgggttct ccatcacttc
    12901 ctgggcggtg aagccctgaa cccgttcgat gcccgaactc acgtatacca agcgcgagat
    12961 cccggtcgcc gcatccacct ccagctggta gatcatgccg ccgggcaggt tgtcgctgat
    13021 gctgcgcagc tgggactccc gctcccgcag cgcgtcctcg gcacggcgcc ggtcggtgat
    13081 gtcccgtgag atgccgaaca ggccgatcac gtgaccggtc tcgtcgcgca tggggccctt
    13141 ggtggccagg aaggtgcgca cgcaaccgtc cgccgtggtc acggtctcct cgaccgtgct
    13201 gaccttccct gccgccagca ccgcccggtc gcgggccatg atcgcgcggg catcctccgc
    13261 gccgaacagc gcggtgtcgt cgcgaccgac gatctcctcc aggggcagcc cgacaaagcg
    13321 cgccgcggcc tcgttggcga ggcggtagct gccgtccaga ttcttgatga agatggcgtc
    13381 cgaggtgcct tcgatgatgt cccgcaggcg tgcctcgctg gcatgcagca tctgctcgta
    13441 gcgccgccgc tcgctgacat cctcgatgat ccagatgctg ccgtcgtggg gcaccgcggc
    13501 atccagcgca ttgcccgtga ggcgcgccca gaaacgccgg ccgtcgcggc cctgcacctc
    13561 cacctcgcgc cggtagggct cgccccgcgc gagcgtggcg tacccctctt cgccgaggga
    13621 gcggtaatcg tccgggtgcg cgtagaagcc acgcatgctg cagccgatca gcgcgcccag
    13681 gtcgtgaccg aacatcgccg caaaatgccg gttggcccac gcgatgcggc ggtcctgcac
    13741 gaagaggatg cccacgctgg cgttgtccag gatcacgttg cgcatgcgca gcagatcgtg
    13801 ggcctccagt tcgaggcgtt tgcgctcggt aatgacgacg gcgttgccca ccacgcgcat
    13861 gctgcggccg tccaggccgc tcaccgcgcg gccgtgggca gagatccagg cgacatcgtt
    13921 cgcggcgcgg atcacccgga actccaccgc aaagctgccg gtcccgcgca gggccgccgc
    13981 cgcggtctca agcaccgcct ggtcatccgg atggacacgc ccgcggaaat ccgcggggct
    14041 gcgcgccgcc ccttcgtgga atccgaacat cgcctccagg gccggcgacc accaggcctc
    14101 accctgctcc ggatgccact cccacacgcc cacgcgcccg cccatctgaa gcagtcgcag
    14161 gcgctcatcg cagcgctcga gcccgctccg cgcctcgtcg tgggccgtca tgtcgcggaa
    14221 ggaccacact tccagggtcc gcccgccaag ggtcacgtgc cgcccgccca gctgcatccg
    14281 ccgcgccgcg ccgcgcacct gcaggaccgc cggaaagtcc cggaccacgc cgtgggcgcc
    14341 cagggcggcc gcgcagcgct gcagatcgcg ttcgtcgccc cacaggccca gcgcgtcctg
    14401 ccgccggccg agggccgccg cgcgcgcaat gccacaggtg gcgacccagg catcgttcac
    14461 ggccaggacc tgcccggaat ccgggtccgc gatcaggagt cccgtgtcca caacgcccaa
    14521 caaggcctcg caatcggccc ccagcggccc aaggggaagc tcagcagcca tgttccggcg
    14581 ctccccagac tgccatcgtt tccatcgtcc gtcctccgcc ccctcgtttc gcgtacttca
    14641 tcggcgccgc cccaccggtc tgaagccggt tgtggcgcgc cgcgtcgccc aggatccgat
    14701 cagaccataa aaaacgggcc ctgcggtgcc agcgaaggca cgggagggcc cgaaaagacg
    14761 aggaggagat tcggcatgcg gccatccgag acaaccgcac cgaacgcttt ggacgatgca
    14821 agagccattc cacaccgtcc cgcgcccgga acggcccgcg ccacgaccct ccgccaccct
    14881 gccgcaggcc ccgaactggc cggaaaacgg gccggactgg tgcatccgcc gtccatgcgc
    14941 acccaaaatg ggcaccctgc cggcgactca catccggccc tccggcgcct ccagccagtc
    15001 caggactgtc gcaaacaaca catccggatt caccggcttg gggatgaaat cgttcatgcc
    15061 ggcctcgtag caggcgcgcc ggtcgtcctc gaacacgttg gcggtcatgg ccagaatcgg
    15121 ggtcgcgccg tgtgcgggca gggcgcggat gcgccgcgcc gcctccagcc cgtccatctc
    15181 gggcatctgc acgtccatca ggatcaccgc gtaggcgcgc cgcgccgcca gggtgaccgc
    15241 ctgggcgccg tcgatggcca ggtccacctc caggcccgcc acctcctgca gcagctcgat
    15301 ggccacctcc tggttgatcc agtcgtcctc cgccagcagc acgcgggccg cgcgatggcg
    15361 gttccgcagg cgcgcctccg cctcggcgcc ggaataggga cgcagcccgc cgtccgggcg
    15421 cgggatgccg gcggccccct gttccaggca gatggtgaag gcaaaggtgc tgccggcacc
    15481 gggctggctg gtcacctcga tgtcgccgcc catcagccgc accagacgcc ggcagatggt
    15541 gaggcccagg ccggtcccgc caaactggcg cgtggtggag ccatccacct gctcgaaggg
    15601 gtcgaagatg cgctggcgcg cctccggtgg aatgccgatg ccggtgtcgg cgacctcaaa
    15661 gcgcacatgc aggccttcgg gtgccgtccg ttcgatcgag gccgccaggg tgacatgccc
    15721 ttccgtcgtg aacttgatgg cgttgcccgc cagattgagc aggatctgct gcaggcgcag
    15781 ggcgtcgcct atcaactcgc gcgcctgcaa ctggggcgcg atgcgctgga tgaggcccag
    15841 gcgcttggcg ctggcacgtt cgccgacgag gctctccacg gcgcccagga ccgtgccgag
    15901 gcggaacggc gcccgctcga gggtgaggcg gtcggcgtcg atcttggaca gatcgaggac
    15961 gtcgttgagg agctgcagca gatgctgggc cgaggtggcg atcttgcgca ggcgctcgca
    16021 ctgggccgga tcgctgctct gccgctccac catgtgggtc aggccgatga tcgcgttcat
    16081 cggcgtgcgc agctcgtggc tcatgttggc caggaaggtg cccttcgccc gattggcggc
    16141 ctcggcggcc tccttggcca ccgacaggtc ggcggtgcgc gctgccacca gctcttccag
    16201 gtggctgcgg tgacgccgca gctccgcctc ggcggccttg cgcgcggtga catcatggaa
    16261 caccgccagc acgtggacgc catcctcgtc gcgggcaacg gtggacgtga ccacctgaac
    16321 gatgcgccgg gtcccgtcgc gggcatgcag ggccagctcc acccccgtca ggtgccgtcc
    16381 tgtcaggatc tgcgccagac gccgccgcgc ccgctccgga cgccgcgggc ggaagaccgt
    16441 gccgatcggc ccatgcacgt tcatcgcttc gagggaatat ccgcagagca gctcgatgct
    16501 cgggttccac accgtgaagc gcacgcccgg cccccccgtc accgcatgga acaccgccac
    16561 cccgtccgcc tccgcgtcga tgaccgcgcg ggtgaatgcg gcctgccgct gcaggctgag
    16621 ctgcagggcc ttgcgctcgc tgatgtccat gtgcgtcccc accagccgtc gggcgcgcgg
    16681 ggtgccgccg gtcaggtcct gcaccgccac ggcgcgggac aggatgtcca cccagtggcc
    16741 gtcccggtga cgcatgcggt attccacctc gaagcgggga atgcggccgt tcaggtaggc
    16801 caccgcgtgc tgcagggtac gttcccggtc gcccggttcc atcaggtccg cccaggtgtc
    16861 caggcgatgg ggcagttcgt cgttgcggta gccgagcatg gaggaccagc gcggcgagaa
    16921 atacacctcg tccgtgtcgg tgttccagtc ccacatgccc tcgttggtcc cgcgcagggc
    16981 gtactccagc cgctcgcggc tcacctggag ctcggtctcc gtctgcttct ggcgggtgac
    17041 gtcctcgtac accccgagca cgccgatgat ccgcccgccg gcatcccgca ggggcaggcg
    17101 cgatccgcgc acccatgccc gcggattgtc ggcggtcatc tggcggcggt cgccggtcgg
    17161 gatcggcaca ccgctcaaca ggatgcggca gtcctcgtgg cggatcgtct cggcggccgc
    17221 gcgccacgcc agatcgccat cctcgcggcc gatcacctgc gccggcccgg ccagcccggc
    17281 atccgccgca aacaggctgt tgcagcccag atagcgggac gcacgatcct tccagaagac
    17341 ccggctgggg atggtctcga ggaccgtctg cagcaggttg cgggactcct ccaccgcgcg
    17401 caggtcctcc acccgctggc tgatgtcccg cccggatgcc agcagcgccg cctcgccccc
    17461 cgccgcctgc acgcgcacgc cgcggaagtg gtacatgcgc agcccgaggc gggtgcgcag
    17521 agggacttcc agatccacct gcccgccggc gaagacggcc gcgatggccg cctccacgcg
    17581 ggcgtggtca gcctcgtcga agtgcgccgt ggcgggcagg gccagcaagg tctcgagggg
    17641 caggccggtc acggccaggg cctgatcgtt ggcccagcgc aaccggcccg ccggatcgat
    17701 gcgcacgagg atgtcaggca ccgcccgcga cagcccctgc agctcctcca gcgcctcgct
    17761 caggcgcgcc tcggcctggc gccgctcgtc gatttcgcgg cggacgcggg cgtaggcgcg
    17821 ggcgccttcg atggccgtgg cgagcttgtc caccaccgcc cgcagttggc ccagggtgcg
    17881 ttcgcccagg gtcgcagcca cctgggaatg aaggcgcagc acgccgtgac cgtcgagggg
    17941 aaacagggcg atcgccccct ccgcctcgcc ttcgccaaaa cacagggcgc cgtgttccgg
    18001 cgtccccgcc gggatgtggc gcggcgtctg ggcggtccgc gcccagcggg ccagggcatg
    18061 cccggacggc agtgtgcccg ccggcactgc gtgacgcggc agcgcatgaa tcagggtcag
    18121 cgccgcggcc tcgtccggcg ccgggtcgtt cccgtcgggg ggctcgaacc accacacgga
    18181 cgcggaggtc aggttgcgcc gggccatcag cacacgcagg aagtcccggc acgtggcggg
    18241 cgcatccagc gtgcgaccga cggtgaggga cagttcatac aacaggagca gctctgcggt
    18301 gatgtcttct tgttgtgcac tcatgcggtc agggccggga tgcggcgatg acaaaggtct
    18361 tgttgtagaa ctccagcagg cgggttccgc ccgacgcgat ctcgcccagg gccagcaccc
    18421 cctccggcac ggcgtcggtg agcgccgcca gggccgtcgc cacgcccgcc agttcgtgcc
    18481 ggaaaccggc gcccagcatc agtgcgcggg aatagcagtc cgagatgaaa taacgggccg
    18541 gccgcggccc ggccgcacac gcctccaccg cctgacgggc cgccgcgacg aggctttccg
    18601 gcgtaccgtg ggcaatgtac agcggcgaat gctcggccac atccgccagc accgcgaggc
    18661 cgccgtcgtc cgtctcgccc atggggtcac ggatcacgtc ctcgctgtcc tcgcgtccga
    18721 tgcacagcgg aaaggccgca cccagatcgg gaaacacggg ccggccggca aaggccggcg
    18781 ccagcgcctc cacgctctgc cggtagaggg cggcggccgg tcgccagttg agttccagaa
    18841 tgctgttggc gcggctgcgc gaggccacga acggccccag gacccgggtc cagccgtggc
    18901 ggacgtgcac ctccatgtgc tcgggcgtga gcaccgccag ggccccgtcg gcccacagac
    18961 cgtccccggt gaagaccacc ggcgcccggc gcaggtcgcg gcaggcagca cccgcgccca
    19021 cgtaacggag gctgccgccg aagcggtcga agagatcgtc gaggaaggcg gcgatgccgg
    19081 ccgccaggct gtccagcagc acgtaggcgc tgtgcgcccc ggcgccggac ggcggcgtgc
    19141 gccagcgcgg gccggcggcg ctcagatccg ccaccgccac ctggctggca ccctgcaggg
    19201 gcagcgcgag gagaccgttg tgcacgaccc gcgtcccgtg gatcagaccc gggaacaccc
    19261 cgccgcagac cctcacgcct tcgccccggc aggactccag cagctccggc acgcggggca
    19321 actcggtgtc ggccacgcac agcagccaca ggggcgactc gccggccacg gcggattggc
    19381 gcagctcggc ggcgacgtgg gcgggagacg cgaagcgcag gtacatggac gtatccggaa
    19441 cgggcgggcc ggccgccgcc tccggacgtt ccggacaggg cggcgaggcg cgatgcggtg
    19501 cattcacttc tcaggctagc agatgcaccg gcgatgcaac atgcctgatg ccgtgtcgcc
    19561 ccccggtcgc cgcccagcac gctttcccgt atcctgcgaa ccgatctccc ccgccacggt
    19621 ccgacctgcc atgaagcccg cgccctgcat ccgcaccgtc gccgtcgccc tcaccggcgc
    19681 ctccggcatg gcctatggcc tgcgcctcac cgaatgtctg ctcgccgccg gctgccgggt
    19741 ctggctgctg gtctcccagg tcgcccaggt ggtggcccgg caggagctgg acctcaccct
    19801 gcccagccgc ccggccgagg cgcaggccct gctcaccgcc cgcttcggcg cggccgaagg
    19861 gcaactgcag gtgttcggcc gggacgaatg gttcgcgccg cccgcctccg gctccaaccc
    19921 gcctgacgcg atggtggtgt gcccctgcac catgggctcc ctcgccgcca tcgccgccgg
    19981 cctggccggc acgctcatcg agcgggctgc cgacgtggtc atcaaggaag gccgtccgct
    20041 gatcctggtg ccgcgggaga cgcccttctc ggcgctgcac ctggagaaca tgctgcgcct
    20101 ggcgcgcatg ggcgtggtca tcctgcccgc caatccgggc ttctaccacc gcccccagag
    20161 cgtggaggcc ctggtggatt tcgtcgtcgc gcgcatcctc gaccagctcc gcgtgcccca
    20221 cagcctgctg ccccgctggg gcgaagacgc aacggagtga acatggagcg cgtcgaactg
    20281 cccctctttc ccctgcgcac cgtcctgttc cccggcggcg tgctgcccct ccggatcttc
    20341 gaagcgcgct acctggacat ggcggcccgc tgcatgaagg ccggaatgcc cttcggcgtg
    20401 tgcctgatcc aggatggcca ggaggtgggc gaggcggcca cgccccacag cgtgggcacc
    20461 accgcccgca tcgtcagctg ggacatgccc cagcagggcg tgctgctggt cacctgccgg
    20521 ggcgaacggc gcttccgcat cgacgcgcgg cagacccacg ccgaccgcct gcagagcgcc
    20581 caggtgcagt tcctcgacgc ggccgacgcg gcgcccaccc cgattcccgc cgggcgggcc
    20641 ggcgtgctgc ccctgctcaa ggccatcgtc agcgatgcgg gcgatgagct gttcccgccg
    20701 ccccacgcct tcgacgacgc cgcctgggtg gtccgccgct tcgccgaggt gctgcccatc
    20761 cccgccctcg cccgccagcg cctgctggaa ctggacgatc cgctgcaggc cctggagatc
    20821 gtccagacct acctgcgcca gcatcagctg gcaagctgaa gcgcgtccgc atccgtcgca
    20881 ccaccacgcg ggctcagccg gcgacggtcg gcgtgccctc cgccgccagc cgctccagca
    20941 gccgccgcac cggcgccggc agcgcagcgt ccgccagcgc acccagccgc agccagacct
    21001 gccccgcgcc gggcgccccg tcggatgccg ccacgtccac ccgttccgga cgcaggtgca
    21061 gcgtgaagtg ggtgaagacg tggcggaaat cgggcaggcg cgcgacacgc accacgtcca
    21121 gcccgaagcg ctgccccacc caggccgcgg agtccacatc ctcgggcagc tccggcaggc
    21181 tcaggagtcc gccccagatg ccccgcggcg gacgccgctc cagcagcacc tgcccgtccc
    21241 gcaggatcgc cgcgaagcgt ccctcgcgca ccacgacggc acgccgcgga cgtggcgccg
    21301 gcagctcggc aacccgctcg tcccgccgcg ccacgcactc gccagccagc ggacagcgcc
    21361 cgcaggcggg ccgggcgcgg gtgcacacgg tggcgcccag atccatctgg gcctgggtgt
    21421 agacgtccac gtcctgcgcc ggcagcaggc gctcggccag cgcccacagg tgctgctcca
    21481 cgtcccgctc gcccggataa ccgtcgatgc cgaagacgcg ggtcagcacc cgcttcacgt
    21541 taccgtccag gatcgctgcc cgcgtgccgt aggcgaaggc cgccaccgcc gccgcggtgg
    21601 agcggccgat acccggcagc gccgccaatt gcgccggatc cgccggcaac acgccgccgt
    21661 ggcgggccat caccgcctgg gcgcaggcat gcaggttgcg ggcacgggcg tagtagccca
    21721 ggccgctcca cagggccagc acgtcctcca ccggcgccgc cgccaggctg gccagatccg
    21781 ggaagcgcgc caggaagcgc ccgtagtagg gcagcaccgt atccacctgg gtctgctgca
    21841 gcatgatttc cgacagccag atgcggtagg cgtcccgggt gccctgccag ggcaggtcgt
    21901 ggcggccatg gatgcgctgc caggcgatca gcgtggcggc aaaagagtgg tgtgttttca
    21961 attcgatacc ccctcgacgg cgacaggacg cagggcgata atgcgggccc ccaacaggtc
    22021 tgcggcgcct ttgcagcgat ccccacccgc catgacagcc ttcaagaacg atcccgccgt
    22081 gcgtgccttc cgcgacaccc tgggcatgtt tcccaccggc gtgaccgtcg tcaccgcccg
    22141 cggcgccgac ggcgcgcccg tgggcctcac ggtcagctcc ttcaattccg tgtccctcgc
    22201 gccgccgctg gtggtgtgga gcctgtccgg ccatctggcc tgcgtgccgg ccttcgaaaa
    22261 cgccgcagtg tacgcgatca acgtcctcgc cgaggaccag caggcgctct cccagcgctt
    22321 cgccagccgc gcggacgaca agttcgccgg cgtcgcctgc accgacggca ccgacggcgt
    22381 gcccctgctc gccggctgct gcgccgtcat ggaatgccgg cccttcgccg tgcatccggg
    22441 cggcgaccac ctggtcttca tcggcgaggt gatccgcttc gagcgggacg ccgagcgcaa
    22501 gcccctggtc ttccagggcg gcgcctaccg ccgcctcgcc tgagtccatg cccgcgccgc
    22561 actggatcga gtccggcggc ggcacccgcg ggctgctctt cctgcacggc gtcagcggcg
    22621 gcgccgaagc ggcccgcacg ctgctgccgc agctcctgcc gcagggctgg cgcggcctgg
    22681 cctgggacat gcccggctac ggcgccagcg cgccgctgga tccactcgat ttcgacaccc
    22741 tcgccgacgc catcgtcacc ctgctggacg ccgccggcct gtcccgcgcc gtgctcgtcg
    22801 gccacagcct gggcggcatg attgccctgc acaccgccgc ccgccacccg gcccgcgtgg
    22861 acgccctggt gctggcctgc tgcacgccgg ccttcggcgc cccggccggg ccgcttcagc
    22921 aggccttcct ggcccgccgc ctgggccccc tggagaccgg cgccacgatg gccgaactcg
    22981 ccgccgacct gattcccgcc atggccgggc cggacgccga cccggcgctg atcggcgccc
    23041 acatcgcgct gatgacgcgc atcccgcccg cccgctacgc cgccgccctg cgcgccctgg
    23101 tgcacttcga cgcccgcgcg gccctgcccg gcctgacgat gccggccctg gcggtggccg
    23161 gccggcacga cagcgtgtcc acgccgcgcg tggtgcgcca gatggccgaa cgcctgcccg
    23221 ccgccaccta cgccgaactc cccgccggcc acctcgcccc cttcgaggcg ccggaggcct
    23281 tcgcccgcct cgtgcgggac ttcctcaccc gcctgcccga ccggccggcc tgaccttcca
    23341 ggatccgctc ccccatggat gaaagcccct tcaaaggcaa gaccggcctg agccgggtgt
    23401 ggaacgcctt ccgctactcc atggccggcc tcgccgccgc ctaccgcaac gaggatgcct
    23461 tccgccagga ggtgtggctc tccgccctgc tgatccccgt cgccctggtc ctgcccgcca
    23521 gcgccgtggg caaggccctg atggtggcca gcgtgctgct ggtggtgatc gtggaactga
    23581 tcaactccgc cgtggaggcg gcggtggacc gggtctccct ggagcgccac cacctcgcca
    23641 agcgggccaa ggacatcggc agcgcggcgg tcttcgtcgc cctggtgaat gtgatcgccg
    23701 tgtggggcct ggtcctcgcc ggctgaggcg ggtcggcgca ccgcgcctcc gccgtctgcc
    23761 tcgcagggcg gagccgccat cgccccgtta agggtcgctt aatctggcgg ggctaaggtg
    23821 gcgcccatca tcgcgtcgct ccgagtccgc ccatgctccg cccccttcgt tacctccccc
    23881 acggcatcgc cgccgcctgt gccctgatcg ccgcctgcgc ggccctcgcc gccgagccct
    23941 tggtcctcca gccggtccag ctcaaggccc tcggcatcga gaccgccgtc gccgccgaag
    24001 ccgccgccgg ccgtggcgcg cgcctgcccg cccgggtgag cgtacccaac gcccagctgc
    24061 gcgtcgtctc cgcgccggtg gaaggcctcg tcgagagcct cgccgtggcg cccggcagca
    24121 cggtgcgcaa gggccaggag gtggcccgcc tggccagccg cgccgcactt gaattgcaac
    24181 gggacgccct ccaggccgac agccaggcgg ccctcctcaa gcacagcctg agccgcgacg
    24241 agaccctgtt ccgcgaaggc ctgatccccg aatcccgcct ccaggccacc cgcgccgccg
    24301 ccgcccaggc gggcgcccag gccgcagagc gccgtcaggc cctgggcctc gccggcgccg
    24361 cggcgggcac gctgggcagc gcgctgatcc tcaccgcgcc cctcgacggc gtggtgctgg
    24421 agcaatccgt gcagaccggt cagcgcgtcg agccggccgc gccgatcttc cgcatcgccc
    24481 gcctgtcgcc cctgtggctg gagatccagg cgcccctgga ggtggccgcc gggctgcgcg
    24541 agggcgcgcc cgtgcgcctc gccgacggcg ccctgcgggg ccgcctgatc gccatcgggc
    24601 gggccgtgga tgccaccagc cagaccgtgc tgctgcgcgc cgaggtgggc gccggggccg
    24661 agcgcctgcg ccccggccag gtggtggacg tggaactgga cgcccccgcc ggcggccccc
    24721 gccgcctgcc tgccgccgcc gtggtgcgcc acgagggcgc cagcctggtg ttcgtgcaga
    24781 ccggcagcga cgcccacggc acccgcttcg agccccgccc ggtgaagatc gtcgccgaag
    24841 gcggcgaagg cctcggcgtg gacggtgtgg ccgccaccga gcgggtggtg gtgaagggcg
    24901 cctccggcct caaggccctg ctcgccggag tcggccggga atgatcctcg acgccctgat
    24961 ccgcttcgcc ctgacccagc gcctgctcct gctggtggcc accgccctgc tcgccgcggc
    25021 cggggtggac gcctacctga acctgcccat cgacgccttt cccgacgtct ccagcaccca
    25081 ggtcaagatc atcctgaagg cgccgggcat gacccccgag gaggtcgaga cccgcatcgc
    25141 cgtgcccgtg gagcaggaga tgctgggcat cccgcgccag cgcctgctgc gctccatgtc
    25201 caagtacgcc ctcaccgaca tcaccatcga cttcgaggac ggcaccgaca tctactgggc
    25261 ccgccagcag gtgggcgaac gcctcgccaa tgccctgggc aacctgccgc ccggcgtcag
    25321 cggcggcatg gcgccgatca ccacgcccct gggcgagatg ttcatgttca ccgtggacgg
    25381 cgagctgccc ctggcggaac ggcgcgccct gctcgactgg gtgatccgcc cgcagctgcg
    25441 cacgatcccc ggcgtggccg acgtgaacag cctgggcggc gaggtgcgca gcttcgaggt
    25501 ggtgccgcgg ccccagtcca tggcggcgcg gggcctcgcc ctcaaggacc tgcaggcggt
    25561 gctggaggcc aacaaccgca acgacggcgc cggccgcctc aacgacgggg aggaaggcct
    25621 gctggtgcgc gccgaagggg cgatccagac cctcgacgac ctgcgcgcca tcgtcgtgca
    25681 ggcccgcgac ggccgcgtga cgcgggtggc cgacgtggcc gaggtgcgct ggggcgccct
    25741 cacccgctac ggcgcggtga gcgccgacgg ccgcggcgag gccgtggagg gcatggtgct
    25801 gggcctgcgc ggcgccgacg ccaagcaggt ggtggcgggc gtcaaggcgc gcctcgccga
    25861 gatcgaaccg accctgcccg agggcgtgca cctcaccgtg ctctacgacc gcagccgcct
    25921 cgtggagcgc gccgtgggca ccgtctccaa ggccctgggc gaagccatcg tgctggtggt
    25981 gctcctgctg ctggccttcc tcggcaacct gcgcgcggcc ctggtggtgg ccctgatgct
    26041 gcccctggcg gccctgtcca ccttcatcct gatgcgcttc gacggcctgt ccgccaacct
    26101 catgagcctg ggcggcctgg ccatcgccat cggcatgctg gtggatgcgg cggtggtggt
    26161 ggtggagaac gtggagaacc agctcgccga agcccaggcc aacctgccca ccctgcacgt
    26221 catctaccgc gccacgcggg aggtggcggt gccggtggcg tcgggcatcg ccatcatcat
    26281 catcgtcttc ctgcctctcc tcaccctgca ggggctggag ggcaagatgt tcgcgccggt
    26341 ggccctgacg gtggtgttcg ccctgttctc gtccctgctg ctgtccctca ccgtggtgcc
    26401 ggtgctggcc tcctggctga tccgccgcgg cgcccaccac gaaccctggc tggtgcgcgt
    26461 gctgaccgcc gcctacacgc gcctgctcga ccgcgccctg gcccgcggcc gccagttcgt
    26521 gctgatcgcc gtggccgggc tggccgccgc cgcggtggcc ttcaccttcc tcggcaagag
    26581 cttcatgccc accatggacg agggcgacct gatcctgcag ctggaaaagc tcccctccat
    26641 cggcctcgac cagagtctgg cggtggacca gcgcctgcag caggccctgc tggcgcgggt
    26701 gccggagatc cgcgccatcg tcgcccgcac cggctcggac gagctgggcc tcgaccccat
    26761 gggcctcaac cagaccgaca ccttcctcgt gctcaccccc cgcgacacct ggcgccacgc
    26821 cgacccggcg tggctggccg accgggtgcg cgaggtgatg gccgacttcc ccggcatcgg
    26881 ctactccttc acccagccca tcgacatgcg cgtctccgag atgctcaccg gcgtgcgcgg
    26941 cgacgtggcc atcaaggtct tcgggccgga cctggcgacc ctcaaccgcc tcgccaaggc
    27001 catcgagacc gccgtccgcg cggtgcccgg cgccgaggac accttcaccc tcaagaacga
    27061 cggcgtgcag tacctgaagg tgagcgtgga ccgcctcgcc gccgggcgct tcgggctcaa
    27121 cgtggacgac atccagaacg acctcaaggc cctgctggaa ggccgtcagg tgggcgtggt
    27181 ggtggagcac ggccggcgcc tgccggtgct gctgcgcggc cccgacgccc tgcgcgactc
    27241 ccccgccgac ttcgccgccc tgcgcctgtc cctgcccgac ggcggcagcg tgcccctcgc
    27301 cgacgtggcc cgcctcaccc gcatcgacgg cccggtcaag gtggaccggg aaaacgccca
    27361 gcgctacgtg gtgatccagt ccaatgtgcg cgaccgggac ctggtgggct tcgtcgagga
    27421 cgcccgcgcc gccgtggccg ccgccgtgcc cctgccggcc ggctaccgcc tggtgtgggg
    27481 cggccagttc gagaaccagc agcgcgccgc cgcccgcctg atggtggtgg tgccggtcgc
    27541 cctggtgctg atcttcctgc tcctgttctc caccttccgc tcggtgcgcc aggccctgct
    27601 ggtcctgtcc aacatcccct tcgccctgat cggcggcgtg ttcggcctgc tcctggccgg
    27661 cgaatacctg tcggtgcccg cctcggtcgg cttcatcgcc ctgctcggcg tggcggtgct
    27721 caacggcgtg gtgatggtga gccacttcaa ccagctgctg gagcgcggcc tgcccctcgc
    27781 cgaggtggtg cgcgaaggcg cccgccgacg cctgcgcccg gtgctgatga ccgccagcat
    27841 cgccgccttc ggcctggtgc ccatgctctt cgccagcggc cccggctccg aggtgcagcg
    27901 ccccctcgcc gtggtggtga tcggcggcct cgtcacctcc accgccctca ccctgatcct
    27961 gctgccggtc ctgttccgcc gcttcggcgt cgccgcagcc cccaccctgc cggaggcccc
    28021 ccgtggctga cgtactcctc accctcatcg tccccaccga catcgcccag cacgtggaag
    28081 acctgctcct gtcccggccg gacctggtgc gcggcttcac caccagcgcc gccgacggcc
    28141 acggcagcgt cgtcaccctc agcggcgcgg aagaactggt gggcggccac gccccgcgca
    28201 tgctgatccg cacggccggc cccgaagacc tgatccgcag cctgatggcc gccgtcaagg
    28261 aggccttccc gcgggccaac ctctactact ggctggtgcc gctcctcgac cgagggcagc
    28321 tatgatccgc cccctcgccc tgctcgccgc cctgctgatc accgcggccc cggccggcgc
    28381 cgcagccccc gccgacacgg ccatcctgcc ccccgacgcc gccgtcgccc gcgtgctccg
    28441 tgcccagccg gccatccagg ccgccgtcag ccagatccgc gccgaagagg ccacccgccg
    28501 ccgcctggaa gccggcccct acgaatgggc cctgcgcctg ggcggccagc agcgcctcac
    28561 ccgcctcccc gccaccccgg aagagcgctt caccgaatgg aacgccgccc tggagcggcc
    28621 cctgcgcctg cccggcaagg ccgccgcaga cgccgagacc ggcgccgccg gcgtcgccct
    28681 ggcggagatc gcccaccagg acatgcgcca cgagaccagc cgcgtcctcc tcgccggctg
    28741 gttcacctgg ctgcgggcgc gcgccggcgc cgaacagtgg gccgcccagg tggccctgct
    28801 ggaacgccag gccgccgccg tgagccgccg ccagcagctc ggcgacgccg cccgcatcga
    28861 gaccgtccag gccgaagccg ccctcgccca ggcccgtgcc caggggggcc aggcccgcag
    28921 ccgcgcccaa gtggccgccg aagacctgcg ccgccgcttc cccggcctgc cggtggacgc
    28981 ccccgcccgg gtgcccgagc ccgcccccgt cctcggcagc gaggacgact ggatcgcccg
    29041 ccagctggaa cacaaccacg agctgggcgt cgcccgcaac gagacccgcc gccagcacgc
    29101 cctggccagc cgcgccggcc acgaacgcag ccccgacccc agcgtcggcg tgcaggtggc
    29161 ccgcgagcgc ggcggcgaag accgcctcgt cggcgccttc gtgagcatcc ccctgcccgg
    29221 cgccgcccgc gccgccagtg ccgacgccgc cctcgcccag gccgacgccg cccaccgccg
    29281 cgaagccgcc gtcctgcaac gcatcaccgc cgaagccgcc gccctctacc gcaacgcagt
    29341 cgccgccgtg gacgcctggc gcagcctgca cgacgccgcc ctccgcctgg acagcgccgc
    29401 caccctgacc gcccgcgcct accagctcgg cgaaggcagc ctcaacgacg tcctcctcac
    29461 ccgccgccag gcccacgaag ccaccctcgc cgccaccacc gcccagctcg acgcgctgga
    29521 gctgggctat cggctgcaac tggatgccca cgcgttgtgg gatctggatg aggagtgaag
    29581 cgggacggcc gtgggcagcg tcccggcgat cacccgagac atccggcccc gtcccctgga
    29641 cgacgtttcg gcggccccaa aagtggggat gcgcatcc
//
LOCUS       DAOQMM010000037        29189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_452_length_29189_cov_48.981396, whole genome shotgun sequence.
ACCESSION   DAOQMM010000037 DAOQMM010000000
VERSION     DAOQMM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29189
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_452_length_29189_cov_48.981396"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLH85_08195"
     CDS             <1..330
                     /locus_tag="PLH85_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNB64582.1"
                     /translation="IDVVMIANLLIMVIIGGYETFVSRLDLDNHPDQPEWLSHVNAGV
                     LKIKLSTAIIGISSIHLLKTFINAGNLPEHTIKWQVIIHVVFLLSALAMALVDKLMNQ
                     TVLLSKH"
     gene            349..1881
                     /locus_tag="PLH85_08200"
     CDS             349..1881
                     /locus_tag="PLH85_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNB64583.1"
                     /translation="MTAIKQEDLIQSVADAFQYISYYHPLDYIKALGEAYDREEAPAA
                     KDAIAQILTNSRMAAEGHRPICQDTGIGMVFIKVGMQVTWPDATMSIQEMINEGVRRA
                     YSNPDNPLRASVLADPAGARKNTKDNTPAVVHFEIVEGHHVEVICAAKGGGSEAKSKF
                     AMLNPSDDLVDWVLHKIPEMGAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIHEL
                     KAKAASGAPLSRAEALRLELMEKINALGVGAQGLGGLTTVLDVKVLDYPCHAANLPIA
                     LVPNCAATRHCHFHLDGSGPAKLDPPKLEDWPAVTWTPDVKSATRVNLDTLTKEEVAS
                     WKVGQKILLNGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVG
                     PAGPTTATRMDKFTRMMLEQTGLISMIGKSERGPVAIEAIKDNNSAYLMAVGGAAYLV
                     AKAIKSAKVVGFEDLGMEAIYEFDVQDMPVTVAVDSTGTSVHNTGPKEWQVKIGKIPV
                     KA"
     gene            2066..3484
                     /locus_tag="PLH85_08205"
     CDS             2066..3484
                     /locus_tag="PLH85_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB64584.1"
                     /translation="MSPEHFLADAERIRQRVIDTLLAEFAELSEGVVIVDRDARIVWM
                     NDKYPRHLMVHDAADIIGRPVEEVIPNSLMRQVVSTGKPIMLDIMELGHESVVVMRLP
                     VRDEHGEVIGGVGMVIFDDARQLAPVVSRFQSLRTELADTRRKLDEARRSKYTFASFI
                     GASPRCVAVKNKARRAARAASPVLILGETGTGKELLAHAIHAASPRAGGPFVAVNIAA
                     VPETLLEAEFFGVAPGAYTGAERKGRSGKFELAQGGTLFLDEIGDMSPALQSKLLRAL
                     QEKEIEPVGSNRLISVDVRIIAATSRDLPKEIEAGRFRADLFYRLNVITLDVPPLRDR
                     LDDLPFIAEALTDSLARQLDEPPRSLSRAALDYLAHHSWPGNVRELANVLERALLMSD
                     ADCLDQADFEQVLPGLVRPPARPVAENARTLDQIQQATERQAIADAIAAAGGNKAQAA
                     RSLGISRASLYEKIAALGIAAP"
     gene            3642..5042
                     /locus_tag="PLH85_08210"
     CDS             3642..5042
                     /locus_tag="PLH85_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNB64585.1"
                     /translation="MSFLIVLAALCFLMFVAYRGYSVILFAPVAALGAVLLTDPTLVA
                     PMFTGLFMDKMVGFVKNYFPVFLLGAIFGKVIELSGFSKSIVSSVIKVVGAERAMLSI
                     VLVCSLLTYGGVSLFVVVFAVYPFAAEMFRQGGIPKRLIPGTIALGAFTFTMDSLPGT
                     PQIQNVIPTTFFKTNIYAAPVLGTVGAVFIFIVGLSYLEWRRRTAKSAGEGYGTELLN
                     EPEPFDETEKLANPVLAILPLVLVGVTNKLFTDLIPQIYGTSHSFVPAVIGTAKPVVQ
                     DVSKIAAIWAVEGALLVGILTVMVFAWRTVSTKFAEGTKAAVGGALLASMNTASEYGF
                     GAVIAALPGFLVVADALKVIPNPLVNEAITVTTLAGITGSASGGMGIALAAMSETFIQ
                     AANAAGIPLEVLHRVASMASGGMDTLPHNGAVITLLAVTGLTHKVAYKDIFAITMIKT
                     TAVFVIIATYYATGLV"
     gene            5079..7082
                     /locus_tag="PLH85_08215"
     CDS             5079..7082
                     /locus_tag="PLH85_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HNB64586.1"
                     /translation="MSISRHPLANSLRASDTGKIVSAREAVNLIRDGDTVATGGFVGI
                     GFPENIAIALEELYLENQGSEGQGLGFPRNLTLVYAAGQGDGKDRGLNHLGHDGLVSR
                     VIGGHWGLVPKLQQLAVANRIEAYNLPQGVITHLFRDIAAGKPGTLTRVGLGTFVDPR
                     FGGGKLNEKTTADVVRLMQIDGEDYLFYKAFPINVGIIRGTTADPDGNITMEKEALTL
                     EAQAIAMAAHNSGGIVIAQVERIAERGTLSSRQVKIPGIFVNCVVVAEKPEYHMQTFI
                     EQYSPGFAGELRVPMSAVPSMEMSERKIIARRAALELRANAVVNLGIGMPEGVASVAA
                     EEKIIDLFTMTAEPGVIGGIPAGGLNFGAAVNTQAIIDQPSQFDFYDGGGLDLAFLGL
                     AQADRLGNLNVSKFGPRLAGAGGFINISQNAKKVVFVGTFTAGNLEIAIQHGKLAILN
                     DGRSKKFVEEVEHRTFSGPEAARRHQPVLYVTERCVFRLDPEGLELIEVAPGIDIERD
                     ILARMAFRPIIRGEPALMDERIFGDGPMNLRPDMLEIPMKDRLSYDAENNLFFVNFEG
                     LAVRTEADIERIRATVEHQLAPIGHKVFAIVNYDRFHIEPELTDDYVGMVKGLMDRHY
                     IDVTRYTSSTFLRLKLGEALARSDVVPHMFASADEAQAGLNQR"
     gene            7283..7867
                     /locus_tag="PLH85_08220"
     CDS             7283..7867
                     /locus_tag="PLH85_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64587.1"
                     /translation="MTLRRTPKVLLALLTATLPALSNAGCFPVAGSVRLLPDPTCAVQ
                     SSVSGVVFTGQCFSVNLALIGFPAGTGYAGTTVEPLAGSNGVPTLTPAVIPVDGQPTL
                     PRQMIQTARSVITLGQTERRHSAATKLYTTDVIVFQPKLSSTGDVMPGPTVEQILIAG
                     TDGKGAYAGATGNLTITGNSIGTPAPVVGKICLP"
     gene            7949..8725
                     /locus_tag="PLH85_08225"
     CDS             7949..8725
                     /locus_tag="PLH85_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64588.1"
                     /translation="MLRRLRIAVLLLILASVALTAWRSQARIEAWRSSLHVTVYPIAA
                     DDAPATRRYIESLTADTFDELEDWFEAEFMRHGKALRRPLAFNLAPPLTALPPPFPAG
                     GSALATARWSLQLRLWAWRHERAPGPAPDVRLFLLFHDPARTPTLAHSVGLEKGRIGL
                     VQLFARRSETPRNAVIIAHELLHTLGAADRYDPVTLHPRWPDGYAEPDRTPRWPQHLA
                     EIMGGRIPVDAGRADIPDGLHEALIGAATAAEIGLSAPRP"
     gene            complement(8737..9045)
                     /locus_tag="PLH85_08230"
     CDS             complement(8737..9045)
                     /locus_tag="PLH85_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNB64589.1"
                     /translation="MDHSRAERRRDRRIPLGCKVAIRVEGTREPVVGVCVELSVGGMT
                     IQSSHVPRMDEVFELVVLPPEPGGVHSPLHVRARVRRCDGTAQPGLYEMGVEIIEVLR
                     "
     gene            complement(9138..9632)
                     /locus_tag="PLH85_08235"
     CDS             complement(9138..9632)
                     /locus_tag="PLH85_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64590.1"
                     /translation="MIRSLQRVAWGSALLVTLAGCSSVSTEPPHDWLIVPAAGMAAVT
                     NGYLKIPALTAAVAAYAVVDPLAPNWVIREAPLGEDRYRLALRMKGIHTGGDGEARQV
                     FARRAAQLAGQPGFGAYEVVAWQEGLDSTRPFSQRVAEGEIRLLRASRALAAMPPPAT
                     SARP"
     gene            complement(9643..10833)
                     /gene="flgL"
                     /locus_tag="PLH85_08240"
     CDS             complement(9643..10833)
                     /gene="flgL"
                     /locus_tag="PLH85_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNB64591.1"
                     /translation="MRISTGMIYDAGVASIQQQNSKLLDAQKQVATGRRVLSPADDPV
                     ASARALEVSQSKSVNDLYASNQAYANDALKLVDSKLGSVTDLLTYVRTRAVESGNGTY
                     SSSEFAAIASDMTQQFSALQSLANSQDSTGEYVFAGYRSNAQPFAGSLAGGITYQGDQ
                     GQRSLQISASRILPVTSSGDQIFNQIRAPAGSAYALAGAANTGTALVSATSLSGYNGH
                     TYAISVGAGPAYQVFDSTADPTHASPITPTVTSSGTTTTLAFGGISMTLTGTPAAGDS
                     YSLGTVAGAFDVLGNFVRSLQSGNAAVTAFGANQAIAGMDAAQDSVSQVRSAVGSRMV
                     EVESQQNVTSAMTLQYSETLSNLQDADYASAVSNLTQRSTYLQAAQQSFMKVSNLSLF
                     NYLS"
     gene            complement(10851..12800)
                     /gene="flgK"
                     /locus_tag="PLH85_08245"
     CDS             complement(10851..12800)
                     /gene="flgK"
                     /locus_tag="PLH85_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNB64592.1"
                     /translation="MGTSLFSIGLTGLNAAQAGLSTTSHNIANVGTAGYSRQSITQST
                     NPAQFTGGGFLGEGTKVDTVKRAYDDFLTRQVLSADTKYNEYDTYNTQIAQLDNLLAD
                     SSAGLTPAYQSFFQGVQEVAANPSSIPARQSLLSAGESLVSRFQTLSDRINEVRSGVE
                     GQIQDTVKSISTYAQQIADLNKRIADAQQAGSSQPANDLLDQRDQAVRELNQLTRVST
                     TVLSDGSMGVFIGTGQPLVMGANATVLQARASQGDPTRFSVNIIAQNGNAITIPESML
                     SGGKLGGLLSLRSETLDSTQNQIGLMAVGLATAFNAQHALGQDLNGNLGTDFFVAPSV
                     SVQPAQGTTTAAPAVTYANIAQLTADDYRLTFTDTTGGYSLTRLSDGTSVSAASVGLS
                     ITPPASSKVGDSFLVQPTRYAAGNMAVALTDTRMIAASAPITTGARSGNLGTGSIGAA
                     VVSSTSSLSSFGTAASPTTLTYSGGNLSGFPAGLPVTYTPPGGSPVTVAAPVASIPYT
                     SGMSIALGGVSFTLSGAIQNNDTFTVSPTVAGVSDSRNAVLLGSLETTKMLLTANGAP
                     SATFQSVYSQMVSAVGNKAREVKVNAEAQNSLLSQATAAQQSITGVNLDEEAANLIRY
                     QQAYQASGKVMSVAAKLFEQVLQLG"
     gene            complement(12886..13836)
                     /gene="flgJ"
                     /locus_tag="PLH85_08250"
     CDS             complement(12886..13836)
                     /gene="flgJ"
                     /locus_tag="PLH85_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNB64593.1"
                     /translation="MAADFSQINALDPRTLGNLQRLAKENSPEAAREAAKQFEGLFLQ
                     QVLKSMRDASPKNGLFDSEESRMYQGMLDQQLALQLASRGGGVGLAKVIERQLGVGRA
                     TADTSGSSPSLDTAIRRSYDAALPAGSAANAAAKSAASAASAPASGLDLPAGAGPRAF
                     VERIWPDAVEASRATGIPARFFVAQAALETGWGKYELKNADGSPSYNLFNIKAGRRWS
                     GDTVSTTTVEYANGTPTRESARFRRYGSYAESFRDYAAMLANNPRYAAVMGQSDASGF
                     ARALQSAGYATDPQYADKLTRIIQGNTLRTALAASASRNA"
     gene            complement(13838..14908)
                     /locus_tag="PLH85_08255"
     CDS             complement(13838..14908)
                     /locus_tag="PLH85_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNB64594.1"
                     /translation="MALCLVAPAVRAERLKDLASIGGVRNNQLVGYGLVVGLDGSGDQ
                     TTQTPFTVQSIINMLGNLGVTLPPGQSLQLKNVAAVMVTASLPPYARPGQQIDVTVSS
                     MGNARSLKGGTLVMTPLKGADGQIYAMAQGSLAVSGAGGASGGSKVTVNHLAAGRIPG
                     GATVERALPSAVGQGEYVFIELNDADFATAQNAVDAINRLAPGSAQAVDARQIQVRAP
                     DDPNQRVAFLGRVENLDVTPAIQAAKVIVNSRTGSVVMNQKVALQNCAVAHGSLTVTV
                     NNEPQVSQPGALSGGQTVVTNKGSVDIKQEGGTLFNLKAGTNLADVVKALNAVGANPL
                     ELIAILQAMKASGALRAELEVI"
     gene            complement(14962..15639)
                     /locus_tag="PLH85_08260"
     CDS             complement(14962..15639)
                     /locus_tag="PLH85_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNB64595.1"
                     /translation="MKSPRALTSLVAVALLAGCSALQTTPPTAVHQPMSIRPEPRTAQ
                     ASQPGAIFQQSFSRPLFEDRRARFVGDIITINLVEQTAASKKSSANAERNASIGASIS
                     ALTKVPLAGLNGLDATASDASKFGGKGDAAANNAFTGTITTTVIEVLPNGNLLVSGEK
                     QIAINQGNEFIRFSGVVNPVNVTGSNTVQSTQVADARIEYRANGYIDETERMGWLQRF
                     FMTALPF"
     gene            complement(15660..16442)
                     /gene="flgG"
                     /locus_tag="PLH85_08265"
     CDS             complement(15660..16442)
                     /gene="flgG"
                     /locus_tag="PLH85_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNB64596.1"
                     /translation="MIRSLWIARTGLDAQQTQLDVISNNLANVSTNGYKRARGVFEDL
                     LYQTLRQPGAQSTQQTTIPSGLQIGTGVRPVATERIHTQGNLNQTGNSLDVAIQGSGF
                     FQILLPDGTTAYTRDGAFQKDANGQIVTSSGYPLQPAITIPSNALSLTIGKDGVVTVT
                     LPNQTAPAQIGNIQVATFVNPGGLQSVGENLYLETAASGTPNPNTPGSNGAGVLNQTY
                     VETSNVNVAEELVLMIQTQRAYELNSRAVQTSDTMLGKLTSL"
     gene            complement(16591..17331)
                     /gene="flgF"
                     /locus_tag="PLH85_08270"
     CDS             complement(16591..17331)
                     /gene="flgF"
                     /locus_tag="PLH85_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNB64597.1"
                     /translation="MDRLIYTAMTGAAATLGQQAAVAHNMANATSTGFRAELHRLRAV
                     PVQNAQLPTRAFVVDASVSDDFSTGPLQHTGHTYDMAIQGKGWFAVQMPDGSEAYTRD
                     GSFEVSANGVLQTRSGLPVLGGGGPITIPPDTEVIVGADGTVSTVPSSGARNTTAVVD
                     QLKLVNPPEQDLQRGGDGLFRLAGGTAAPADPAVRVASGYLEGSNVNVVEQMVSMISL
                     SRQFEMQTKMLTTAQENDRSAAQVITKA"
     gene            complement(17354..18922)
                     /locus_tag="PLH85_08275"
     CDS             complement(17354..18922)
                     /locus_tag="PLH85_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNB64598.1"
                     /translation="MAFQQGLSGLSIASKALDAISNNVANSGTVGFKQSAAQFSDVFA
                     ASLASGSSVQVGIGASIDKVSQQFTQGNITTTSNALDLAINGQGFFRMSDNGTISYSR
                     NGQFQTDKNGFIVNSSGLHLTGKVADANGVLTGTTGDIKLNFSDASPSATTAANYNVN
                     LDSRAATPSALAAAVVTGTAVPSLTVGASPANVLTITVDGAPTATSVTIAPSTYADVG
                     SLVSAIQTAVDQTALKGRVKISSDSTGKISVTSARSGRLSDVTVGGAAATALNLPGTT
                     ATSSNSGFSTSDPTSYTSTTSQTVYDSLGNAHTQSLYFVKTAQSNVWDVYTSLDGGFP
                     PEINPATGVHTPTTISFDANGVLQTPTSFATSYTISTGAVVPLDFTVELKGTTQFGNS
                     YGVNQLSQDGYTTGKLSGLTVSADGTIQGNFSNGQSRVMGQVWLASFQNPNGLQSLGG
                     NQWAVTNASGPEQPNAPGTGSLGVVQSAAVEDSNVDLTSELVNMITQQRAYQANAQSI
                     KTQDQVLQTLVNLR"
     gene            complement(18936..19610)
                     /locus_tag="PLH85_08280"
     CDS             complement(18936..19610)
                     /locus_tag="PLH85_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNB64599.1"
                     /translation="MSTTVNSTTTSDFVASLQKRASTSSTTTTDAQSKFLTLLTEQLK
                     NQDPLNPMDNAQMTSQLAQISTVDGIEKLNTTLASLLQDSQSNAALQAAALVGQNVMV
                     AGKDLTLSGSKGYGGIELPSAADKVTVTIKDANGLQVRKLELGARDAGVSDFAWDGTT
                     DSGSKAVDGAYTISVTASRGDTALKPVALELTKVGSVLRTASGGVSLDVGNGSLVSLS
                     DIKQIL"
     gene            complement(19630..20034)
                     /gene="flgC"
                     /locus_tag="PLH85_08285"
     CDS             complement(19630..20034)
                     /gene="flgC"
                     /locus_tag="PLH85_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNB64600.1"
                     /translation="MSMLNVFQVAGSALHAQSTRLNTTASNLANADSVVSSNGQPYRA
                     KQVVFAATPMGNPAAQGVRVTQVVESAAPLRMVYDPKSPAANADGYVAMPNVNVVEEM
                     TNMISASRAYQTNADVMGTAKSLLEKTLTIGQ"
     gene            complement(20045..20449)
                     /gene="flgB"
                     /locus_tag="PLH85_08290"
     CDS             complement(20045..20449)
                     /gene="flgB"
                     /locus_tag="PLH85_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNB64601.1"
                     /translation="MTDRLDNELRFHQTALNARAYRQQVIASNIANADTPNFKARDLD
                     FREALKGALGGGTANLDLRTTHAAHLPGAVATPMAAALKFRNEEQGAVDGNTVNMDVE
                     RSAFAENAVQYQASVTFINGLLRSMQLAVQGQ"
     gene            20787..21509
                     /gene="flgA"
                     /locus_tag="PLH85_08295"
     CDS             20787..21509
                     /gene="flgA"
                     /locus_tag="PLH85_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNB64602.1"
                     /translation="MPNRSPVRPMHRLSIRLLLLLIGLAAAAVASARQDAAPVRAEVA
                     AFLQQQTRSLAGEVRIEVGEFAPDNGLAPCTRLEAFLPPGARAWGRVTAGIRCLAPTP
                     WSAYVPAQVRVTGPYLITTTALAAGQALGPGDVRVEVGEVSAQPADVLTHPTQAAGQI
                     ARLAIAAQRPLVASLLRAPVAVAQGQAVKVITRGPGFQVANDGTALSGGSDGQPVQVR
                     LGNGQVVRGTARVGGYVEIALP"
     gene            21618..21932
                     /gene="flgM"
                     /locus_tag="PLH85_08300"
     CDS             21618..21932
                     /gene="flgM"
                     /locus_tag="PLH85_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNB64603.1"
                     /translation="MKIDNSVKSAAGLPSGDGRTRNAKESPKTQGSSGGGEKVDISSL
                     SARMQQMEEVISNTPVVDSAKVDEIKQAISDGRFKVDTDKVADGLIESVRQMLSAQTG
                     QA"
     gene            21945..22421
                     /locus_tag="PLH85_08305"
     CDS             21945..22421
                     /locus_tag="PLH85_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNB64604.1"
                     /translation="MTGHPVLTERLARLVRDEHTQLAGFVELLKQEEALLVENSIDPL
                     LTLAGEKTERYRGLQRLSDERIVLFARMGIPVSGEAIRATLAPDPAALAEWEAVVDLA
                     REAHARNALNGKLITERLQNNQQALSTLLLAAEQPQIYGPDGQARPTGGSRHLGSV"
     gene            complement(22548..23438)
                     /gene="motD"
                     /locus_tag="PLH85_08310"
     CDS             complement(22548..23438)
                     /gene="motD"
                     /locus_tag="PLH85_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNB64605.1"
                     /translation="MGRRRGRHEEEHENHERWLVSYADFITLLFAFFVVMYSLSSLNE
                     GKYRIMSDSVVNAFRNLAVNSNSDRMIASPIPNLRPPTPQAADAAKTEEARQARAERV
                     RNMAEEIRKVLAPLVADGQVRVTEGAFGITVEINASVLFAPGEAQLGPDAVRALRAVA
                     QVVAAASFPVTVEGHTDNTRIATAMFPSNWELSAVRASSVVRLFVESGVAPDRLTAAG
                     YGDQRPVADNLTPDGRARNRRVTLLIESRVAEPSPDGTVRASGRTDSILPADALPPMP
                     RTQDASAAPAVPASPVPPRP"
     gene            complement(23449..24195)
                     /locus_tag="PLH85_08315"
     CDS             complement(23449..24195)
                     /locus_tag="PLH85_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNB64606.1"
                     /translation="MDSISLIGITLALAAILVGQVLEGGHIGSLVQPTAFLIVIGGTL
                     GAVMLQSSLRVFRDGLRMLRWVFMPPALDHATLIQKVVSWSVTARKEGLLALEAQIPA
                     LDDPFIAKGLQLLVDGVEPERLREVLEVEIDTWEARMKLGAKIWEAAGGYAPTIGILG
                     AVMGLIHVMENLSDPSKLGSGIAVAFVATIYGVGSANLIFLPMSKKLMGVIATLVTLR
                     EMVVDGLVGIANGDNPRIIESRLSGYAGNA"
     gene            complement(24224..24988)
                     /locus_tag="PLH85_08320"
     CDS             complement(24224..24988)
                     /locus_tag="PLH85_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNB64607.1"
                     /translation="MYTASGTLDKEQLVIQYAPLVKRIAYHLMAKLPASVQVEDIIQN
                     GMLGLLDAINRYEEGLGAQFETYAVQRIRGAMLDGLRENDWLPRSLRRDMRRIEAAVH
                     QLEQQNGRPPTETELAGSLGMPLADYQHMLQEARGYQLVYFEDFSGDGDEDYLERHIT
                     IEGNDPLTLLMDSNMRDVLIQGIDDLPEREKTVMGLYYEQDMNLREIGEVLGVSESRV
                     CQLHSQAIARLRSRIAGAVNGTAPVKVKGRGRRAAG"
     gene            complement(25079..25864)
                     /locus_tag="PLH85_08325"
     CDS             complement(25079..25864)
                     /locus_tag="PLH85_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FleN"
                     /protein_id="HNB64608.1"
                     /translation="MHAHSDQAAGLRRLFRRTPPAVFALFATGRSPLKTAARAVRSLT
                     EGGRRVVVLEEHGEATGSLFGSFGTDGGADLLATLERGLDPADTLREAADGLWLVRAA
                     ATAQAVPWLDAGHLSRLESAIGELQRRADLLAVHAAGDGAGLTPFAQAAARRLLVVEA
                     SGVGARGACQWIKGLAAAGAGSLEVAVSGARDRADATALFASLSDYTARHVGLPLVWR
                     GELERDGLAEAMSTPLGARHPAEGVQAFVRRLRAWSAQASAAG"
     gene            complement(25864..27405)
                     /gene="flhF"
                     /locus_tag="PLH85_08330"
     CDS             complement(25864..27405)
                     /gene="flhF"
                     /locus_tag="PLH85_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNB64609.1"
                     /translation="MNVKRYFAKTAREALRQLKEELGPDAVVLSNRAVNGGVEILALP
                     ANDVAALQRARQQAAAAAPAAPVIPAAASTEVPRAAARASQPAADFFDDDFRLSLSRA
                     AAARTQPAAAPAAAPRRPAPVAATAPRAPEMQPFNPPRVELGRDVFHAAAPARKPAEP
                     VRSPLRGDPAAPREAAPRPRATGIEEEARIRALEAANSQMATELAAIRGMIERQLAGF
                     AWGELNRTAPSRTRLMSDLLEAGFSAALARELADAVPEDASVEDAHRILRTVLSQRLR
                     AMSSDADIIDRGGVYALVGPTGVGKTTTTAKLAARCVVRHGADKLALITTDGYRIGAH
                     EQLRIYGRILGVPVFVVRDATDLRETLAGLRDRHMVLIDTMGMSQRDKMVAEQAAMLT
                     GAGKVKRLLLLNATSRGDTLDDVINAYAGDDLQGCIFTKTDEAMSVAPVLDVAIRHQV
                     EVFYVANGQRVPEDLHLPNRDTLLAQALKELPEPSPFRLAPMEAGLVMAGSPAAAVHA
                     AGGAR"
     gene            complement(27553..>29189)
                     /locus_tag="PLH85_08335"
     CDS             complement(27553..>29189)
                     /locus_tag="PLH85_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNB64610.1"
                     /translation="TLDAMPGKQMAIDADLNAGLIDEKEAKRRRAETAQEADFYGAMD
                     GASKFVRGDAVAGILIMGINVVGGLIVGVIQHNMELGQAANNYTLLTIGDGLVAQLPA
                     LIISVAAGLVVSRVGDEGDMGSQVVGQVFSNPQVLVLTAAILGVLGLIPGMPNFVFLL
                     LAGGLGALAWYGGKRQAAIAEEEVPAAAAPAVPPEAQEASWTDVTPVDVLGLEVGYRL
                     IPMVDKAQDGELLKRIRGLRKKFARDIGFLSSAVHIRDNLELKPNAYRIVLKGVEVGN
                     GMAFPGQFMAINPGRVSGPLNGRETTDPAFGLPAVWVDAGQREQAHALGYTVVDASTV
                     VATHLNHLVLSHAAELLGRTETQALLDHIAKDNPKLVEDLVPKLLPLATVQRVLQNLL
                     DEGVNIRDMRSIVEVLAECAPRVQDPVELTTRVRQALGRAIVQGLFPGNGEIQVMALD
                     PGIERVLLQAIGGGGPEGGAIEPGLADTLLRETAVAAQRMEDIGLPPVLLVPPALRWL
                     LSRFLRRSVPTLKVVSNAEVPETRLIRVASMITVAR"
BASE COUNT         4552 a         9824 c        10041 g         4772 t
ORIGIN      
        1 atcgacgtgg tgatgatcgc caacctgctg atcatggtga tcatcggcgg ctacgagacc
       61 ttcgtctcgc gcctcgatct ggacaaccac ccggaccagc cggagtggct ctcccacgtg
      121 aacgccggcg tgctcaagat caagctgtcc accgccatca tcggcatctc gtcgatccac
      181 ctgctcaaga ccttcatcaa cgccggcaac ctgcccgagc acaccatcaa gtggcaggtg
      241 atcatccacg tcgtattcct gctgtccgcc ctggccatgg cgctggtgga caagctcatg
      301 aaccagaccg tcctgctgtc caaacactaa cgagagggag cctcacccat gaccgccatc
      361 aaacaggaag acctgatcca gtccgtcgcc gacgccttcc agtacatcag ctactaccac
      421 ccgctggatt acatcaaggc cctgggcgag gcgtacgacc gggaagaggc gcccgccgcc
      481 aaggacgcca tcgcccagat cctgaccaac tcgcgcatgg ccgctgaagg ccaccgtccg
      541 atctgccagg ataccgggat cggcatggtc ttcatcaagg tcggcatgca ggtcacctgg
      601 cccgacgcca ccatgagcat ccaggagatg atcaacgaag gcgtgcgtcg cgcctacagc
      661 aacccggaca accccctgcg cgcctccgtg ctggccgacc ccgccggcgc ccgcaagaac
      721 accaaggaca acacccccgc ggtggtgcac ttcgagatcg tcgaaggcca ccacgtggaa
      781 gtgatctgcg cggccaaggg cggcggctcc gaagccaagt ccaagttcgc catgctcaac
      841 ccctccgacg acctggtgga ctgggtcctg cacaagatcc ccgagatggg cgccggctgg
      901 tgtccgccgg gcatcatcgg catcggcatc ggcggcacgc ccgagaaggc catgctgctg
      961 gccaaggaat ccatcatggc gccggtggac atccacgaac tcaaggccaa ggccgcctcc
     1021 ggcgccccac tcagccgcgc cgaagcgctg cgcctggagc tgatggaaaa gatcaacgcc
     1081 ctgggcgtcg gtgcgcaggg cctgggcggc ctcaccaccg tgctggacgt gaaggtgctg
     1141 gactacccct gccacgccgc caacctgccc atcgccctgg tgcccaactg cgccgccacc
     1201 cgccactgcc acttccacct ggacggctcg ggccccgcca agctggaccc gcccaagctg
     1261 gaagactggc ccgccgtcac ctggacgcct gacgtcaagt ccgccacccg cgtcaatctg
     1321 gacaccctga ccaaggaaga agtcgcctcc tggaaggtcg gccagaagat cctcctcaac
     1381 ggcaagatgc tcaccggccg cgacgccgcc cacaagcgca tcgccgacat gctggccaag
     1441 ggcgagaagc tgccggtgga cttcaccaac cgggtcatct actacgtcgg accggtcgac
     1501 ccggtgcgtg acgaggtcgt cggcccggcc ggccccacca ccgccacccg catggacaag
     1561 ttcacccgca tgatgctcga gcagaccggc ctgatctcca tgatcggcaa gtccgagcgc
     1621 ggccccgtgg ccatcgaggc gatcaaggac aacaactccg cctacctgat ggccgtcggc
     1681 ggcgctgcct acctggtggc caaggccatc aagtccgcca aggtggtggg cttcgaggac
     1741 ctgggcatgg aagccatcta cgagtttgac gtgcaggaca tgcccgtcac cgtggcggtg
     1801 gactccaccg gcacctcggt gcacaacacc ggcccgaagg aatggcaggt caagatcggc
     1861 aagatcccgg tcaaggcctg attccctcgc ctcggcgacg tccctgcagc ccggccccgc
     1921 gccgggctgt gtcgttgacg ggcccggcat cagggtgaac ccgtatcggg cggtgccgcg
     1981 cgggtgcgct atgctgcacc cacgcccgga ggagacaggc cgttccggcc ccaccggcgc
     2041 ccccacaaga acaaggctgc ccaccatgtc gcccgaacac ttcctcgcgg acgcggagcg
     2101 catccgccag cgcgtcatcg acaccctgct cgccgaattc gccgagctgt ccgaaggcgt
     2161 ggtcatcgtg gaccgggacg cccgcatcgt gtggatgaac gacaagtatc cacgccatct
     2221 catggtccat gacgccgccg acatcatcgg ccgcccggtg gaagaggtca tccccaacag
     2281 cctgatgcgc caggtggtga gcaccggcaa acccatcatg ctggacatca tggaactggg
     2341 gcacgaatcc gtcgtcgtca tgcgcctgcc ggtgcgcgac gagcatggcg aggtcatcgg
     2401 cggcgtgggc atggtcatct tcgacgacgc ccgccagctc gccccggtgg tctcccgctt
     2461 ccagagcctg cgcaccgaac tggccgacac ccggcgcaag ctcgacgagg cccgccgcag
     2521 caaatacacc tttgccagct tcatcggcgc cagcccgcgc tgcgtggccg tcaagaacaa
     2581 ggcccggcgc gccgcccgcg cagcctcgcc ggtcctcatc ctgggcgaga ccggcaccgg
     2641 caaggagctc ctcgcccacg ccatccacgc cgccagcccc cgcgccggcg gccccttcgt
     2701 ggcggtcaac atcgccgccg tgccggaaac cctgctggag gccgaattct tcggcgttgc
     2761 gccgggtgcc tacacgggcg ccgagcgcaa ggggcgcagc ggcaagttcg aactcgccca
     2821 gggcggcacc ctcttcctcg acgagatcgg cgacatgtcg ccggcgctcc agtccaagct
     2881 gctgcgcgcc ctgcaggaaa aggagatcga gccggtcggc tccaaccgcc tgatcagcgt
     2941 ggacgtgcgc atcatcgccg ccaccagccg cgacctgccg aaggagatcg aggccggccg
     3001 cttccgcgcc gacctgttct accgcctcaa cgtcatcacc ctcgacgtac cgcccctgcg
     3061 cgaccgcctg gacgacctgc ccttcatcgc cgaagccctc accgacagcc tcgcccgcca
     3121 gctcgacgag ccgccccgca gcctgagccg cgccgccctg gactacctgg cccaccacag
     3181 ctggcccggc aacgtgcgcg agctggccaa cgtgctggag cgggccctgc tgatgtcgga
     3241 cgccgactgc ctcgaccagg ccgacttcga acaggtgctg cccgggctgg tccgaccgcc
     3301 cgcgcgcccc gtcgccgaaa acgcacgcac cctcgaccag atccagcagg ccacggagcg
     3361 gcaggccatc gccgacgcca tcgccgcggc cggtggcaac aaggcccagg ccgcacgcag
     3421 cctgggcatc tcccgcgcat ccctctacga gaagatcgcc gcgctgggca tcgccgcgcc
     3481 ttgaagctgt ccggctccgc ggacagtgcc gcctgccgca ccggacacga cgcaccgcaa
     3541 catcgcaaaa actgcttaca aatcaacccc aaaaaagcag gcatggaatc tgcagtgaca
     3601 ccccgacagt tttgcatttt aacgacggag gagatctcac catgtcattc ctgattgtat
     3661 tggcagcctt gtgcttcctg atgttcgtag cctaccgggg ctacagcgtc atcctgtttg
     3721 cgccggtcgc ggccctcggc gccgtgctgc tcaccgaccc gaccctggtc gccccgatgt
     3781 tcaccggcct cttcatggac aagatggtcg gcttcgtgaa gaactacttc ccggtcttcc
     3841 tgctcggcgc catcttcggc aaggtcatcg aactgtccgg cttctccaag tccatcgtct
     3901 cctccgtcat caaggtggta ggggccgagc gggcgatgct ctccatcgtg ctggtgtgct
     3961 cactgctcac ctacggcggc gtgtccctgt tcgtggtggt cttcgcggtg tatccgtttg
     4021 ccgccgagat gttccgtcag ggcggcatcc ccaagcgcct gatccccggc accatcgcgc
     4081 tgggcgcctt caccttcacg atggactccc tgcccggcac gccgcagatc cagaacgtga
     4141 tccccacgac cttcttcaag accaacatct acgccgcccc ggtactgggc accgtgggcg
     4201 cggtcttcat cttcatcgtc ggcctcagct accttgagtg gcgtcgtcgc accgccaagt
     4261 ccgcgggcga aggctacggc accgaactgc tgaacgagcc ggaacccttc gacgagaccg
     4321 agaagcttgc caatccggtc ctcgccatcc tgcccctggt actggtgggc gtcaccaaca
     4381 agctgttcac cgacctcatc ccgcaaatct acggcaccag ccactccttc gtgccggccg
     4441 tgatcggcac cgccaagccg gtggtccagg acgtctccaa gatcgccgcc atctgggccg
     4501 tggaaggcgc cctgctggtg ggcatcctca cggtcatggt attcgcctgg cgcaccgtct
     4561 ccaccaagtt cgcagagggc accaaggctg ccgtgggcgg cgccctgctg gcgtcgatga
     4621 acaccgcgtc cgaatacggc ttcggtgccg tcatcgccgc gctgccgggc ttcctggtgg
     4681 tggccgacgc cctcaaggtg atccccaatc cgctggtgaa tgaagccatc accgtcacca
     4741 ccctggccgg tatcaccggt tccgcgtccg gcggcatggg cattgccctg gcggccatgt
     4801 cggagacctt catccaggcg gccaacgccg cgggcatccc gctggaagtg ctgcaccggg
     4861 tcgcctccat ggccagcggc ggcatggaca ccctgcctca caatggcgcc gtcatcacac
     4921 tgctggccgt caccggtctg acccacaagg tggcctacaa ggacatcttt gccatcacga
     4981 tgatcaagac cactgcggtg ttcgtcatca tcgccaccta ctacgccacc ggcctcgtat
     5041 aacaacgctc gcggacctgc ccgggaggag acaccaccat gagcatttca cgccatcccc
     5101 tcgccaactc cctgcgcgcc tcggacaccg gcaagatcgt gtccgcgcgg gaggcggtca
     5161 acctgatccg tgacggcgac accgtcgcca ccggcggctt cgtcggcatc ggctttccgg
     5221 agaacatcgc catcgccctg gaagagctgt acctggaaaa ccagggcagc gaaggccagg
     5281 gcctcggctt cccgcgcaac ctgacgcttg tctacgccgc cggccagggc gacggcaagg
     5341 accggggcct caaccacctg ggccacgacg gcctggtgtc gcgggtcatc ggcggccact
     5401 ggggcctcgt gcccaagctg cagcagcttg ccgtggccaa ccgcatcgaa gcctacaacc
     5461 tgccccaggg cgtcatcacc cacctgttcc gcgacatcgc cgccggcaag cccggcacgc
     5521 tgacgcgggt cggcctcggt accttcgtag acccacgctt cggcggcggc aagctcaacg
     5581 agaagaccac cgccgacgtg gtccgcctga tgcagatcga cggcgaggac tatctgttct
     5641 acaaggcctt cccgatcaac gtgggcatca tccgtggcac caccgccgac ccggacggca
     5701 acatcaccat ggagaaggag gccctcaccc tggaggccca ggccatcgcc atggcggccc
     5761 acaactccgg cggcatcgtc atcgcccagg tggagcgtat cgccgagcgg ggcaccctga
     5821 gttcgcgcca ggtgaagatc cccggcatct tcgtgaactg cgtggtggtg gccgaaaagc
     5881 ccgaatacca catgcagacc ttcatcgagc agtacagccc gggctttgcc ggcgagctgc
     5941 gggtgcccat gtccgccgtt ccgtccatgg agatgagcga acgcaagatc attgcccgcc
     6001 gcgccgcgct ggagttgcgc gccaacgcgg tggtgaacct gggcatcggc atgcccgagg
     6061 gcgtcgcctc ggtggctgcc gaagagaaga tcatcgatct cttcacgatg accgccgagc
     6121 cgggcgtgat cggcggcatt cccgccggcg ggctcaactt cggcgccgcg gtgaacaccc
     6181 aggccatcat cgaccagccc agccagttcg acttctacga cggcggcggg ctggacctgg
     6241 ccttcctcgg tctcgcccag gccgaccgcc tgggcaatct caacgtgtcc aagttcgggc
     6301 cacgcctcgc aggcgccggc ggcttcatca acatcagcca gaacgccaag aaggtggtct
     6361 tcgtcggcac cttcaccgcc ggcaacctgg agatcgccat ccagcacggc aagctcgcca
     6421 tcctcaacga cggtcgctcc aaaaaatttg tcgaggaggt ggagcaccgc accttcagcg
     6481 gcccggaagc cgcgcggcgc caccagccgg tgctctacgt caccgagcgc tgcgtgttcc
     6541 gtctcgaccc ggagggcctg gagctgatcg aggtggcgcc gggcatcgac atcgaacggg
     6601 acatcctcgc ccgcatggcc ttccgcccca tcatcagggg cgaaccagcc ctgatggacg
     6661 aacgcatctt tggcgatggc cccatgaacc tgcgccccga catgctggag atccccatga
     6721 aggatcgcct cagctacgac gccgagaaca acctgttctt cgtcaatttc gagggcctgg
     6781 cggtacgcac cgaagcagac atcgagcgca tccgcgcaac ggtggagcac cagctcgccc
     6841 ccatcggcca caaggtgttc gccatcgtca attacgaccg cttccacatc gaaccggaac
     6901 tcaccgacga ctacgtgggc atggtcaagg gtctgatgga ccgccattac atcgacgtga
     6961 cgcgctacac ctccagcacc ttcctgcgtc tcaagctcgg cgaggccctc gcccgcagcg
     7021 acgtggtgcc ccacatgttc gccagcgccg acgaggccca ggcgggcctc aaccagcgct
     7081 gacaagcccc cgcccccacg aggaacccgg ctccgagccg ggtttttttc gccttcaaat
     7141 cggcagaact gcaagatcct gcaaaaccca cacgcaattt cagcgagtcg ctatagtgcg
     7201 ctccatggcg tctacgggcg cccgcccacc cggtcacacc cttgccgaca accctgtcca
     7261 ccctgccacc cgaggaaccg tcatgaccct ccgccgcacc ccgaaagtcc tgctcgccct
     7321 gctcacggcg accctgccgg ccctgtccaa cgccggctgc ttccctgttg ccggcagcgt
     7381 tcggctgctg cccgacccga cctgcgcggt ccagtcgtcc gtcagcggcg tcgtgttcac
     7441 cggccagtgc ttcagcgtca atctcgccct gatcggattt ccggccggca ccggctatgc
     7501 cggcaccacc gtggaacccc tggcaggctc caacggcgtg ccgaccctga ccccggcggt
     7561 gatcccggtg gacggccagc ccaccctgcc ccgtcagatg atccagaccg cccgctcggt
     7621 gatcaccctc ggccagaccg agcgtcgcca cagcgcggcc accaagctct acaccaccga
     7681 cgtgatcgtc ttccagccca agctcagcag caccggcgac gtgatgccgg ggcccacggt
     7741 ggagcagatc ctgatcgccg gcaccgacgg caagggtgcc tacgccggcg ccaccggcaa
     7801 cctcaccatc accggcaaca gcatcggcac gcccgcaccg gtcgtcggca agatctgtct
     7861 gccctgatcc gctcacgggt ccggcctcgc cggacccttc tccggacgac gatggccatc
     7921 gccccgtcgt catgctcgcc cgtcctccgt gctccgccgc ctgcgtatcg ccgtcctgct
     7981 gctgatcctc gccagcgtcg ccctgaccgc ctggcgcagt caggcccgca tcgaggcttg
     8041 gcgcagttcg ctgcacgtca cggtctatcc gatcgccgcg gacgacgccc ccgcaacccg
     8101 gcgttacatc gaaagcctca cggccgacac cttcgatgaa ctcgaagact ggttcgaagc
     8161 ggagttcatg cgccatggca aagccctgcg acgtcccctt gccttcaacc tcgccccgcc
     8221 cctgaccgcg ctgccgcccc ccttcccggc cgggggcagc gcccttgcca ccgcgcgctg
     8281 gagcctgcag ctgcgcctgt gggcctggcg ccatgagcgg gccccgggcc ccgcgccgga
     8341 cgtgcgcctg ttcctgctct ttcatgatcc ggcgcgcacg ccgacgctgg cgcattcggt
     8401 cggactggaa aagggccgca tcggcctcgt gcagctcttt gcccggcgca gcgagacccc
     8461 gcgcaatgcg gtgatcattg cccacgagct gctccacacc ctgggcgccg ctgatcgcta
     8521 cgaccccgtc acgctgcacc cccgctggcc tgacggctac gccgaacccg accgcacgcc
     8581 gcgctggccg cagcacctgg cagagatcat gggcggacgg attccggtgg acgccgggcg
     8641 cgcggacatc ccggacggcc tgcacgaggc cctgatcggc gccgccacgg ccgcggagat
     8701 cggcctgagc gcaccccgcc cgtgacgccg cggggcctaa cgcagcactt cgatgatctc
     8761 gacacccatc tcatagagac cgggctgagc cgtgccgtca cagcgccgca cccgggcgcg
     8821 cacatggagc ggggaatgga cgccacccgg ttcgggcggc aggacgacga gctcgaacac
     8881 ctcgtccatg cgcggcacgt ggctggactg gatggtcatg ccgcccacgc tcagctcgac
     8941 gcagacgccc accaccggct cccgtgtgcc ctcgacccgg atggcgacct tgcatcccag
     9001 aggaatgcgc cggtcgcgcc gtcgttcggc cctgctgtga tccatgttcg tagagtttcc
     9061 cttggcgtga agcgcgcacc ttacggaatg cgccgcggcc ccgcaaggtt cagagacgct
     9121 tgcaggcgga tgaccgctca gggccgggcc gacgtggccg gcggaggcat tgctgcaagc
     9181 gcccgcgagg cgcggagcag gcggatctcg ccttcggcca cgcgctggga gaaggggcgc
     9241 gtactgtcca gtccctcctg ccaggccacc acttcgtagg ccccgaaacc gggctggccc
     9301 gccagctgcg ccgcgcggcg ggcgaagacc tggcgggcct ccccgtcgcc gccggtgtgg
     9361 atgcccttca tgcgcagggc cagccggtag cggtcctcgc ccaggggcgc ctcgcggatc
     9421 acccagttgg gcgccagcgg atcgaccacc gcataggccg ccacggcggc ggtcagtgcc
     9481 gggatcttca ggtagccgtt ggtgacggcc gccatgccgg cggccggcac gatgagccag
     9541 tcgtggggcg gctcggtgct gacactgctg cagccggcca gcgtgacgag gagcgcgcta
     9601 ccccaggcca cgcgttgcag ggaccgaatc atgaccggcc tctcagctga ggtagttgaa
     9661 gagggacaga ttgctcacct tcatgaagga ctgctgggcg gcctgcaggt aggtggagcg
     9721 ctgggtcagg ttgctcaccg ccgacgcata gtccgcgtcc tgcaggttcg acaaggtctc
     9781 ggagtattgc agggtcatgg cgctggtgac gttctgctgg ctctccactt ccaccatgcg
     9841 cgaaccgacg gcggagcgca cctgggacac gctgtcctgg gccgcgtcca tgcctgcgat
     9901 ggcctggttg gcgccgaacg cggtcaccgc cgcgttaccg gactggagcg atctgacgaa
     9961 gttgcccagc acgtcgaagg cgcccgccac ggtacccagg gaatagctgt cgccggccgc
    10021 cggcgtgccc gtgagcgtca tgctgatgcc gccaaaggcc aaggtggtgg tggtgccgct
    10081 ggaggtgacg gtcggggtga tcggtgacgc gtgggtcggg tcggccgtgg agtcgaagac
    10141 ctgataggcc ggtccggcac cgacgctgat ggcgtaggtg tggccgttgt agccgctcag
    10201 ggaggtggcc gacaccaagg cggtcccggt gttggcggca ccggcgaggg cataggcgga
    10261 gccggccggc gcgcggatct ggttgaagat ctgatcaccg gagctggtca ccggcaggat
    10321 gcgcgaggcc gagatctgca ggctgcgctg gccctggtcg ccctggtaag tgatgccgcc
    10381 ggccaggctg ccggcaaagg gttgggcatt gctgcgatag ccggcgaaga cgtactcgcc
    10441 ggtggagtcc tggctgttgg cgagggactg gagcgccgag aactgctggg tcatgtcgga
    10501 cgcgatggcg gcaaactccg agctgctgta ggtcccgttg ccggattcga cggcgcgggt
    10561 gcgtacgtag gtgagcagat cggtcaccga acccagcttg ctgtcgacca gcttgagggc
    10621 gtcgttggcg taggcctggt tggacgcgta gagatcattc acgctcttcg actgggagac
    10681 ttccagcgcc cgcgccgagg ccaccggatc gtccgccggc gagagcacgc gccggccggt
    10741 cgccacctgc ttctgggcat cgagaagctt gctgttctgt tgctggatcg acgcgacgcc
    10801 cgcgtcgtag atcatgccgg tactgatgcg catgagggtt ctcctggttc ctagccgagc
    10861 tgcagcactt gctcgaagag cttggccgcc acgctcatga ccttgccgct ggcctggtat
    10921 gcctgctgat aacggatcag attggccgct tcttcatcga gattcacgcc ggtgatggac
    10981 tgctgggcgg cagtggcctg ggacagcagg gaattctggg cctcggcatt gaccttcacc
    11041 tcccgcgcct tgttgccgac ggcactgacc atctgggaat acacggactg gaaggtggcc
    11101 gacggggccc cgttggccgt cagcagcatc ttggtggttt ccagcgagcc gagcagcacc
    11161 gcgttgcggc tgtcggacac gcccgccacg gtgggggaaa ccgtgaaggt gtcgttgttc
    11221 tggatcgcgc cggacagggt gaaggacacg ccaccaaggg cgatgctcat gccgctggtg
    11281 tagggaatgg acgccaccgg cgccgccacg gtgacgggcg agccgccagg gggcgtgtag
    11341 gtgaccggca ggccggcggg aaagccgctg aggttgccgc cggaataggt cagggtcgtc
    11401 ggcgaggctg ccgttccgaa gctggacagg ctgctcgtac tgctcaccac ggctgcaccg
    11461 atactgccgg tgcccaggtt gccgctacgg gcgccggtgg tgatgggcgc ggacgcggcg
    11521 atcatgcgcg tgtcggtgag ggccacggcc atgttgcccg ccgcgtagcg ggtgggctgg
    11581 acgaggaagc tgtcccccac cttggagctg gccggcgggg tgatgctgag gccgacggag
    11641 gcggcgctga ccgaggtgcc gtcggacaga cgggtcaggg agtaaccgcc cgtggtgtcg
    11701 gtgaaggtga gcctgtagtc gtcggcggtg agctgggcga tgttggcgta agtcacggct
    11761 ggcgccgccg tggtcgtgcc ctgggcaggc tggaccgaca cgctgggcgc gacgaagaag
    11821 tccgttccga gattaccgtt cagatcctgc ccgagcgcgt gctgggcgtt gaaggcggtg
    11881 gccagcccga cggccatcag gccgatctgg ttctgggtgc tgtccagagt ctcgctgcgc
    11941 aaggacagga gtccgcccag cttgccgccg ctcagcatgc tctcgggaat tgtgatggcg
    12001 ttgccgttct gggcgatgat gttgacgctg aagcgcgtcg gatccccctg ggaggcccgg
    12061 gcctgaagca cggtggcgtt ggcgcccatc accagcggtt gcccggtacc gatgaagacc
    12121 cccatggagc cgtcgctcag gacggtggtg gacacgcggg tgagctggtt gagctcgcgc
    12181 accgcctggt cgcgctggtc cagcagatcg ttggcgggct gactggagcc agcctgctgg
    12241 gcgtcggcga tgcgcttgtt caggtcggcg atctgctggg cataggtgct gatggacttg
    12301 accgtatcct ggatctggcc ctccacgccg gagcggactt cgttgatgcg gtcggacagg
    12361 gtctggaagc gcgacaccag actctcgccg gccgacagca gggattgccg cgcgggaatg
    12421 ctggacggat tggcggccac ttcctgcacg ccctggaaga aggactggta ggccggcgtg
    12481 agaccggcgc tggaatccgc cagcagattg tcgagctggg cgatctgggt gttgtaggtg
    12541 tcgtactcgt tgtacttggt gtcggcgctc agcacctgac gggtcaggaa gtcgtcgtag
    12601 gcgcgcttga ccgtatcgac cttggtgcct tctcccagaa agccgccgcc ggtgaactgg
    12661 gctgggttgg tgctctgggt gatggactgg cggctgtagc cggccgtgcc cacgttggcg
    12721 atgttgtggc tggtggtgga cagccccgcc tgggcggcat tgaggccggt gagtccgatg
    12781 ctgaacaggc tggtgcccat gatccgcgtc cttctgtcgg aagtgtctgc ggagggaaca
    12841 gcaagatctc tgccagcctc gcgccaagcg gcacggaacg tgggctcagg cgttgcggga
    12901 ggcgctcgcc gcgagagcgg tgcgcagggt gttgccctgg atgatgcggg tcagcttgtc
    12961 cgcgtactgg ggatcggtgg cgtagccggc cgattgcagc gcgcgggcga agccgctggc
    13021 gtcgctctgg cccatcaccg ccgcgtagcg cgggttgttg gcgagcatcg ccgcgtagtc
    13081 gcggaaggat tcggcatagg agccataacg ccggaagcgc gcggattcgc gggtcggcgt
    13141 gccattggcg tactccacgg tggtggtgct caccgtgtcg ccggaccacc gccggccggc
    13201 cttgatgttg aagaggttgt agctcgggct gccgtcggcg ttcttcagtt cgtacttgcc
    13261 ccagccggtt tccagcgccg cctgggcaac gaagaagcgg gccgggatgc cggtcgcacg
    13321 gctggcctcg acggcgtccg gccagatccg ttcgacgaag gcccgtggcc cggcaccggc
    13381 cggcagatcg agcccgcttg ccggggcaga cgctgccgat gcggcagatt ttgccgccgc
    13441 gtttgccgca ctgccggcgg ggagggcggc gtcatagctg cgccggatgg cggtgtccag
    13501 cgacggcgaa ctgcccgacg tgtcggcggt tgcgcgcccg acgccgagct ggcgttcgat
    13561 gaccttggcc agacccacgc cgccgccccg gctggcgagt tgcaaggcca gctgctggtc
    13621 gagcatgccc tggtacatgc gcgattcttc gctgtcgaag agcccgttct ttggcgaggc
    13681 atcgcgcatg gacttgagca cctgttgcag gaacaggcct tcgaactgct tggccgcttc
    13741 cctggccgcc tcgggggaat tctccttggc gagacgctgg agattgccca gggtgcgcgg
    13801 gtcgagggcg ttgatctggc tgaagtcggc ggccatgtca gatcacttcc agttcggccc
    13861 gcagggcgcc gctggccttc atcgcctgaa ggatggcgat gagttcgagg ggattggcgc
    13921 cgaccgcgtt gagggccttg accacgtcgg ccaggttggt gccggccttc aggttgaaga
    13981 gggtgccgcc ttcctgcttg atgtccaccg agcccttgtt ggtgaccacg gtctggccgc
    14041 ctgaaagggc gcccggctgg ctcacctggg gctcgttgtt caccgtgacg gtgaggctgc
    14101 cgtgggccac cgcgcagttc tgcagggcca ccttctggtt catcaccacc gagccggtgc
    14161 gggaattgac gatgaccttg gcggcctgga tggccggcgt cacgtccaga ttctcgacgc
    14221 ggccgaggaa ggccacgcgc tggttgggat cgtccggcgc gcgcacctga atctggcgcg
    14281 catccaccgc ctgggcgctg cccggggcga ggcggttgat ggcgtccacg gcgttctggg
    14341 cggtggcgaa atcggcgtcg ttgagctcga tgaacacgta ctcgccctgc cccaccgcgc
    14401 tgggcagggc ccgctccacg gtggcgccgc cgggaatgcg gccggcggcc aggtggttga
    14461 cggtgacctt gctgccgccg gacgccccgc cggcgccgct caccgccaga ctgccctggg
    14521 ccatcgcgta gatctgcccg tcggcgccct tgaggggcgt catgaccagg gtgccgccct
    14581 tgagggaacg ggcgttgccc atggacgaga cggtcacgtc aatctgctgg cccggccggg
    14641 catagggcgg caggctggcg gtgaccatca cggcggccac gtttttgagc tgcagggact
    14701 ggcccggcgg cagggtgacg cccaggttgc cgagcatgtt gatgatgctc tgcacggtga
    14761 agggcgtctg ggtggtctgg tcgccggagc cgtcgaggcc caccaccagg ccgtagccca
    14821 ccagctggtt gttgcgcacc ccgccgatgc tggcgaggtc tttcaggcgc tcggcgcgga
    14881 cagccggtgc gacgaggcac agggccaccg caagggcgaa acagagcgtg cggacggaca
    14941 ggggtttcac gatggcgatc ctcagaacgg cagcgcggtc atgaagaagc gttgcagcca
    15001 gcccatgcgc tcggtctcgt cgatgtagcc gttggcccgg tattcgatgc gggcgtcggc
    15061 cacctgggtg gactggacgg tgttggaacc ggtgacattg accggattga ccacgccgga
    15121 gaagcggatg aactcgttgc cctggttgat ggcgatctgc ttttcaccgg acaccaacag
    15181 gttgccgttg ggcaggactt cgatcacggt ggtggtgatg gtgccggtga aggcgttgtt
    15241 ggccgccgcg tcgcccttgc caccgaactt gctggcgtca gaggcggtgg cgtcgagccc
    15301 gttgagcccg gccaggggca ccttggtcag ggccgagatg gaggctccga tggaggcgtt
    15361 gcgttcggcg ttggccgagg acttcttgct cgcggcggtc tgctccacga ggttgatggt
    15421 gatgatgtcg cccacgaagc gggcacgccg gtcttcgaac aggggccgcg agaaggattg
    15481 ctggaagatc gcgccgggct gggaagcctg cgccgtgcgc ggctcggggc ggatgctcat
    15541 gggttgatgc acggcggtgg ggggcgtggt ctgcagggcg gagcagccgg ccagcagcgc
    15601 gacggcgacg agggacgtca gggcacgggg ggatttcatg gtgcgtctcc ggcctggact
    15661 tacagggacg tgagcttgcc gagcatggtg tcggaggtct gcacggcgcg ggagttgagt
    15721 tcgtaggcgc gttgggtctg gatcatcagc accagttcct cggccacatt gacgttggag
    15781 gtttccacgt aggtctggtt gagcacgccg gcgccgttgc tgcccggcgt gttggggttg
    15841 ggtgtgccgc tggcggcggt ttccaggtag aggttctcgc ccacgctctg caggccgccg
    15901 ggattgacga aggtggcgac ctggatgttg ccgatctggg ccggcgcggt ctggttgggc
    15961 agggtcaccg tgacgacgcc gtccttgccg atggtgaggc tgagcgcgtt ggacgggatg
    16021 gtgatggccg gctgcagcgg gtagccgctg gaagtgacga tctggccgtt ggcgtccttc
    16081 tggaaggcgc cgtcgcgggt gtaggcggtg gtcccgtcgg gcagcaggat ctggaagaag
    16141 ccggacccct ggatggccac atcgagactg ttgccggtct ggttcaggtt gccctgggtg
    16201 tggatgcgct cggtggcgac gggacgcacg ccggtgccga tctgcaggcc ggagggaatg
    16261 gtggtctgct gggtggactg ggcgcccggc tggcgcaggg tctggtagag gagatcctcg
    16321 aagacgccac gggcgcgctt gtagccgttg gtggagacgt tggccagatt gttggagatg
    16381 acgtcgagct gggtctgctg ggcgtcgagg ccggtgcggg cgatccagag ggagcggatc
    16441 atggcgggct cctgagagtc ggggccgccc cgggggcggc aaaggttgcg acgggttcca
    16501 tgctagtccg tgcaagctcc gttccaggcg cggagcagga ccgggaaagg ccgtcaattc
    16561 ggacgcccgg atcggccgcc gggtgccgga tcaggccttg gtgatgacct gggcggcgga
    16621 gcggtcgttt tcctgggcgg tggtgagcat cttggtctgc atctcgaact ggcgggacag
    16681 ggagatcatg ctgaccatct gctcgaccac gttgacgttg ctgccttcca ggtagccgga
    16741 agcgacccgc acggcaggat ctgccggggc ggcagtgccg ccggcaagcc ggaagaggcc
    16801 gtcgccgccc cgctgcagat cctgctcggg cgggttgacg agcttgagct ggtccacaac
    16861 cgcggtggtg ttgcgggcgc cgctgctggg caccgtggag acggtgccgt cggcgccgac
    16921 gatgacctcg gtgtccggcg gaatggtgat gggcccgccg ccgcccagca caggcaggcc
    16981 gctgcgggtc tggagcacgc cgttggcgct gacctcgaag ctgccgtcgc gggtataggc
    17041 ctcgctgcca tcgggcatct gcacggcgaa ccagcccttg ccctggatgg ccatgtcgta
    17101 ggtgtggccg gtgtgctgga gcggcccggt gctgaagtcg tccgaaacgc tggcgtccac
    17161 cacgaaggcg cgggtcggca gttgggcgtt ctggaccggc actgcgcgca ggcgatgcag
    17221 ctcggccctg aagccggtgc tggtggcgtt ggccatgttg tgggccaccg cggcctgctg
    17281 gccgagggtg gcggcggcgc cggtcatggc ggtgtagatc aggcggtcca tggtcgagtc
    17341 ccgtcaggcc ggctcagcgc aggttcacca gggtctgcag cacctggtcc tgggtcttga
    17401 tggactgggc gttggcctgg taggcgcgct gctgggtgat catgttcacc agttcggagg
    17461 tgagatccac gttggagtcc tccaccgcgg cggactggac gacacccagg ctgccggtgc
    17521 ccggcgcgtt gggctgttcc gggccggagg cattggtcac ggcccactgg ttgccgccga
    17581 gggactggag tccgttgggg ttctggaagg acgccagcca cacctggccc atcacccgcg
    17641 actggccgtt gctgaagttg ccctggatgg tgccgtcggc gctgaccgtg aggccgctca
    17701 gcttgccggt ggtgtagccg tcctggctga gctgattgac gccgtaggag ttgccgaact
    17761 gggtggtgcc cttcagctcc accgtgaaat ccaggggcac gacggcgccg gtggagatgg
    17821 tgtagctggt cgcgaaggag gtgggcgtct gcaacacgcc gttggcgtcg aagctgatgg
    17881 tggtgggcgt atggacgccg gtggccggat tgatctcggg cgggaaaccg ccgtccaggc
    17941 tggtgtagac gtcccacacg ttggactggg cggtcttcac gaaatacagg gactgggtgt
    18001 gggcgttgcc cagggagtcg tacaccgtct gggaggtcgt gctggtgtag ctggtggggt
    18061 cggaggtgct gaatcccgaa ttgctggagg tggccgtggt gcccggcaga ttgagtgcgg
    18121 tggccgccgc accgccgacc gtcacgtcgg acaggcgccc gctgcgcgcc gaggtgacgc
    18181 tgatcttgcc ggtgctgtcg ctggagatct tgacccggcc cttcagcgcg gtctgatcaa
    18241 cggcggtctg gatggcggaa acgagggagc ccacgtccgc gtaggtgctc ggggcgatgg
    18301 tgaccgacgt ggccgtcggg gcaccgtcaa cggtgatggt cagcacattg gccggcgagg
    18361 cccccacggt gagggacggg accgccgtgc cggtgaccac cgcggcggcc agcgcggagg
    18421 gcgtcgcggc gcgggaatcc aggttgacgt tgtagttggc ggcggtggtg gccgacgggc
    18481 tggcgtcgct gaaattgagc ttgatgtcgc cggtggtgcc ggtgagcacg ccgttggcgt
    18541 cggccacctt gccggtcaga tgcaggcccg acgaattgac gatgaagccg ttcttgtccg
    18601 tctggaactg cccgttgcgg ctgtagctga tggtgccgtt gtcgctcatc cggaaaaaac
    18661 cctggccgtt gatggccagg tccagcgcat tgctggtggt ggtgatgttg ccctgggtga
    18721 actgctggga caccttgtcg atggaagcgc cgatgcccac ctgcaccgag ctgcccgacg
    18781 cgagagaggc ggcgaacacg tccgagaact gggccgcgga ctgcttgaaa ccgacggtgc
    18841 cggagttggc gacgttgttg ctgatggcgt cgagggcctt ggaagcgatg gaaaggccgc
    18901 tcaaaccttg ctggaaagcc atgttctgtc tccgctcaga gaatttgctt gatgtccgac
    18961 aggctcacca gggagccgtt gccgacgtcg agggatacgc cgccgctggc ggtgcgcagc
    19021 acgctgccaa ccttggtcag ttccagggcg acgggcttga gcgccgtgtc gccgcgggag
    19081 gcggtcacgc tgatggtgta ggcgccgtcc accgccttgc tgccgctgtc ggtggtgccg
    19141 tcccaggcga aatcggacac gccggcatcg cgggcaccca gttccagctt gcgcacctgc
    19201 aggccgttgg cgtccttgat ggtcacggtg accttgtccg ccgccgacgg cagctcgatg
    19261 ccaccgtagc ccttgctgcc ggacagggtc agatccttgc ccgccaccat cacgttctgt
    19321 cccaccagcg cggccgcctg cagggccgcg ttggactggg agtcctggag caggctggcg
    19381 agggtggtat tgagcttctc gatgccatcc acggtgctga tctgagccag ctgggaggtc
    19441 atctgggcgt tgtccatggg gttgagcgga tcctggttct tcagctgctc cgtcagcagc
    19501 gtcaggaact tgctctgggc atccgtggtg gtggtggaac tcgtgctggc gcgcttctgc
    19561 aggctggcga cgaagtccga ggtggtcgtg gaattgacgg tcgtgctcat ggtggcgatc
    19621 ttccggggct tactgaccga tggtcagggt tttttccagc aggctcttgg cggtgcccat
    19681 cacgtcggcg ttggtctggt aggcgcggga ggcggagatc atgttggtca tctcctccac
    19741 cacattgacg ttgggcatcg ccacatagcc gtcggcgttg gcggccgggc tcttcgggtc
    19801 gtagaccatg cgcagcggcg cggcgctctc caccacctgg gtcacccgca caccctgagc
    19861 ggccgggttg cccatgggcg tggcggcaaa gaccacctgc ttggcccggt agggctggcc
    19921 attgctggag acgacgctgt cggcgttggc caggttgctg gcggtggtgt tgagccgggt
    19981 ggattgggca tgcagcgcac tgccggccac ctggaagacg ttgagcatgc tcacgattca
    20041 gtcctcactg accctgcacg gcgagctgca tggagcgcag cagcccattg atgaaggtga
    20101 cgctggcctg gtactggacc gcattctcgg caaaggcgct gcgctccaca tccatattga
    20161 ccgtgttgcc atccacggcg ccctgctcct cgttgcggaa cttgagggcc gccgccatgg
    20221 gcgtggcgac cgcaccgggc agatgcgccg cgtgggtggt gcgcagatcg aggttcgccg
    20281 tgccgccccc cagcgcgccc ttcagggcct cgcggaaatc caggtcgcgg gctttgaaat
    20341 tgggcgtatc cgcattggcg atgttggacg cgatcacctg ctggcgatag gcgcgcgcat
    20401 tgagcgcggt ctggtggaag cgcagttcgt tgtccagtcg gtcggtcatt tccggtcacc
    20461 tctcacgttg ctgggcgggc gtgccgtcat gcatgcattc gggggcggcg cccgatgaag
    20521 ccagtgcacc ttccatgcca gccatgctaa agaggcgcct ggcgggacat ttggctgaac
    20581 agcagcgcat tcccagccca attcgtccgc ggggcgcagc agccggccgc ccgaacaccg
    20641 gcggccctac ggcaggcctg accggcaggg cggcaaagtt tgccgcccgg cagaaccccc
    20701 gcgcggcaca tcccccgaaa tgcccccgaa agtccggtca cttccgggcg acgtccacct
    20761 ccccggcatg ctccaatgga ctcaccatgc cgaaccgctc gcccgtccgc cccatgcacc
    20821 gcctgtcgat ccgcctgctc ctcctgctga tcggtctggc ggctgcggct gtggcctccg
    20881 cccgccagga cgcggcgccc gtgcgcgccg aggtcgccgc cttcctccag caacagaccc
    20941 gcagcctggc cggagaggta cgaattgaag tgggtgaatt tgcgccggac aacggactcg
    21001 cgccctgcac ccggctcgaa gccttcctcc cgccgggcgc ccgcgcctgg ggtcgcgtca
    21061 cagccggcat tcgctgcctt gccccgaccc cctggtcagc ctacgtgccg gcccaggtac
    21121 gggtcaccgg cccctatctg atcaccacca cagcactggc cgccggacag gcgctggggc
    21181 caggggacgt gcgcgtcgag gttggcgaag tcagcgccca gccggccgac gtgctcaccc
    21241 atccgaccca ggctgcgggt cagatcgccc gcctggccat cgccgcccag cgccccctgg
    21301 tcgccagcct gctgcgcgcg cccgtcgcgg tcgcccaggg gcaggcggtc aaggtcatca
    21361 cccgcgggcc cggctttcag gtggccaatg acggaacggc cctgtcgggc ggcagcgatg
    21421 gccagcccgt gcaggtgcgc ctcggcaacg gtcaggtcgt ccgtggcacc gcccgcgtcg
    21481 gcggctatgt ggagattgcc ctgccctgag ccccgcagcg gactgccacg acgcacgcat
    21541 gcccgatccc ggatgggtta aagttttgtc ccatggggtc gttgacttcc ccgactcgtg
    21601 gaagaaaggg ctatgccgtg aagatcgaca actccgtgaa gtccgccgcc ggcctgccgt
    21661 cgggggatgg acgtacgcgc aacgccaagg aatcgcccaa gacccagggc agttccggcg
    21721 gtggcgaaaa ggtggacatc tcctccctgt ctgcacgcat gcagcagatg gaggaggtta
    21781 tttccaacac tccggtcgtg gacagtgcca aggtggatga gatcaagcag gccatcagcg
    21841 acggtcgctt caaggtggac accgacaagg tggccgacgg gctcatcgag agcgtccgcc
    21901 agatgctctc cgcccagacc gggcaggcct gatcccccac gcccatgacc ggccatcctg
    21961 tgctcaccga acgcctcgcc cgcctggtgc gggacgagca cacccagctg gccggcttcg
    22021 tggaactcct caagcaggag gaagccctgc tcgtggaaaa cagcatcgac ccgctgctca
    22081 ccctggcggg cgaaaagacc gaacgctatc gtggcctgca acgcctgtcg gacgagcgaa
    22141 tcgtgctgtt tgcgcggatg ggcattccgg tcagcggtga ggccatccgg gcgacgctgg
    22201 cacctgaccc ggcggccctc gccgagtggg aggcggtggt tgacctcgcc cgcgaggccc
    22261 atgcgcgcaa cgcgctcaat ggcaagctca tcactgaacg cctgcagaac aaccagcagg
    22321 ccctgagcac cctcctgctg gcggccgaac aaccccagat ctacggcccg gacggtcagg
    22381 cccgcccgac cggcggcagc cgccacctcg gctcggtctg accttcccct gcggacaccc
    22441 gccgccaccg tccccgactg acagcgcccc gtccggcacg cgcatccgcg catgcccact
    22501 tcgccatgcg cctgcgtggc ggagcgccat cccgccggcc ccgggtctca aggtcggggt
    22561 ggcaccggtg acgcgggcac ggcgggcgcc gcggacgcgt cctgcgtccg cggcattggc
    22621 ggcaacgcat ccgccggcag gatcgaatcc gtccgccccg aggcccgcac cgtgccgtcc
    22681 ggcgacggct cggccacccg ggattcgatg aggagggtca cccgccggtt gcgtgcccga
    22741 ccgtccggcg tgaggttgtc tgccaccgga cgctggtcgc catagccggc ggccgtcaga
    22801 cgatccggcg caacgccgct ctcgacgaac aggcgcacca cgctcgacgc ccgcaccgca
    22861 gacagctccc agttggacgg aaacatcgcg gtggcaatgc gggtgttgtc ggtatgccct
    22921 tccacggtca ccggaaagct tgctgcggcc accacctggg ccaccgcgcg cagggcgcgt
    22981 accgcatccg gcccgagctg ggcctcgccg ggcgcgaaga gcacgctggc gttgatctcc
    23041 acggtgatcc cgaaggcgcc ttcggtgacc cgcacctgcc cgtcggccac caggggggcc
    23101 agcaccttgc ggatctcctc ggccatgttg cgcacccgct cggcgcgcgc ctggcgggcc
    23161 tcttcggtct tggccgcatc ggccgcctgg ggtgtcggcg gccgcaggtt ggggatgggg
    23221 gaggcgatca tccggtcgct gttactgttt accgccagat tgcggaaggc attgaccacc
    23281 gaatcggaca tgatgcggta cttgccctca ttgagcgacg acagggagta catcaccacg
    23341 aagaaggcga acagcagggt gatgaagtcg gcgtaggaga caagccagcg ctcgtggttc
    23401 tcgtgctcct cctcgtgccg gccacgacgg cgccccatgg cggctccttc aggcgttgcc
    23461 ggcgtagccg gacaggcgtg actcgatgat gcgcgggttg tcgccgttgg cgatacccac
    23521 cagaccatcc accaccatct cgcgcagggt gacaagggtg gcgatgacgc ccatcagctt
    23581 cttggacatg ggcagaaaga tcaggttggc tgagcctacc ccgtagatgg tggccacgaa
    23641 ggccacggcg atgccggaac ccagcttgga cgggtccgag agattctcca tgacgtggat
    23701 caggcccatc acggccccca ggatgccgat ggtcggcgcg taaccgccgg cggcctccca
    23761 gatcttggcg cccagtttca tgcgcgcctc ccaggtgtcg atttccacct ccagcacttc
    23821 ccgcagacgc tccggctcga cgccgtccac cagcagctgc aggcccttgg cgatgaaggg
    23881 atcgtcgagg gccggaatct gggcctccag cgccagcaga ccttccttgc gggcggtgac
    23941 gctccagctc acgaccttct ggatcagggt ggcatgatcg agggccggcg gcatgaagac
    24001 ccagcgcaac atgcgcaacc cgtcgcggaa cacccgcagg gagctctgca gcatcaccgc
    24061 accgagggtg ccgccgataa cgatgagaaa ggccgtcggc tggaccaggg agccgatgtg
    24121 gccgccctcc agcacctgcc ccaccaggat cgcagccagg gcgagggtga tgccgatcag
    24181 gctgatgctg tccatggacg cgcctccgct gccgggtgag cgctcagccg gcagcgcgcc
    24241 ggccgcggcc cttgaccttg accggcgcgg tgccattcac tgcgcccgcg atgcgcgagc
    24301 gcaggcgcgc gatggcctga ctgtgcagct gacacacgcg cgattcggac acgcccagca
    24361 cctcgccgat ttcccgcagg ttcatgtcct gctcgtaata cagacccatc acggtctttt
    24421 cccgctccgg cagatcgtca atgccctgga tcagcacgtc gcgcatgttg ctgtccatca
    24481 gcagggtgag cgggtcgttg ccctcgatgg tgatgtgacg ttccaggtag tcctcgtcgc
    24541 catcgccgga aaaatcctcg aaatacacca gctgatagcc gcgggcctcc tggagcatgt
    24601 gctggtaatc ggccaggggc atgccgaggg agccggccag ttcggtctcc gtcggcgggc
    24661 ggccgttctg ctgttcgagc tgatgcaccg ccgcctcgat gcggcgcatg tcgcggcgca
    24721 ggctgcgcgg gagccagtca ttttcgcgca ggccgtcgag catggcgccg cggatacgct
    24781 ggaccgcgta ggtctcaaac tgggcgccga gcccttcctc gtagcggttg atggcgtcga
    24841 gcaggcccag catgccgttc tggatgatgt cctccacctg cacgctggcc ggcagcttgg
    24901 ccatcaggtg gtaggcgatg cgcttgacca gcggtgcgta ctggatcacc aactgttctt
    24961 tgtcgagggt gccggatgcg gtgtacatcg ttcgtcagct tcgtgaaagc gcctcagggc
    25021 ggaaaaggac cccgccgggc ggggacacgt catcgggggg cctgcaggat tcgggacctc
    25081 atcccgccgc gctggcctgg gccgaccagg cccgcagtcg tcgcacgaag gcctgcacgc
    25141 cttctgccgg gtgacgcgcg ccgaggggtg tgctcatggc ctcggccagg ccgtcccgct
    25201 ccagctcgcc gcgccacacg agtggaagcc ccacatgacg ggcggtgtaa tcggacaggc
    25261 tggcaaacaa tgcggtggcg tcggcccggt cgcgggcgcc gctgacggcg acttccagcg
    25321 acccggctcc ggccgcggca agccccttga tccactggca ggcaccgcgc gcacccacgc
    25381 cgctggcctc caccaccagc agccggcggg cggcggcctg ggcaaagggt gtgaggccgg
    25441 caccatcacc ggcggcatgg accgccagca gatcggcgcg gcgttgcagt tcgccaatgg
    25501 cggattcgag gcggctgagg tgaccggcat ccagccaggg cactgcctgg gcggtggcgg
    25561 cagcgcgcac cagccacagg ccgtcggccg cctcgcgcag ggtgtcggcc gggtcgagcc
    25621 cgcgctccag agtggccagc aggtcggcac cgccgtcggt gccgaagctg ccgaagaggg
    25681 agccggtggc ctcgccgtgc tcttccagca ccaccacccg gcgtccgcct tcggtgaggc
    25741 tgcgcaccgc gcgggcggcg gtcttgaggg gggaccgccc ggtcgcgaag agggcaaaga
    25801 ccgccggtgg cgtgcggcgg aacagacggc gcaggccggc ggcctgatcg gaatgggcgt
    25861 gcatcaccgg gcacctcccg cagcgtgaac ggcggccgcc gggctgcccg ccatcaccag
    25921 gccggcttcc atgggggcga gacgaaacgg cgacggctcg ggcagctcct tgagggcctg
    25981 ggccagcagg gtgtcccggt tgggcaggtg cagatcctcg ggcacgcgct gaccattggc
    26041 cacatagaag acctccacct ggtggcggat cgccacgtcg agcaccggcg ccacggacat
    26101 ggcctcgtcg gtcttggtga agatgcagcc ctgcaggtcg tcgccggcgt aagcgttgat
    26161 gacgtcgtcc agggtgtcgc cgcggctggt ggcgttgagc agcagcagac gcttgacctt
    26221 gcccgcgccg gtgagcatgg cggcctgctc ggcgaccatc ttgtcgcgct ggctcatgcc
    26281 catggtgtcg atgagcacca tgtggcggtc gcgcaggccg gccagggtct cgcgcagatc
    26341 ggtggcgtcg cgcacgacga acaccggtac gcccaggatc cggccgtaga tgcgcagttg
    26401 ctcgtgggcg ccgatccggt agccgtcggt ggtgatgagg gccagcttgt cggccccgtg
    26461 gcggaccacg cagcgggcgg caagcttggc ggtggtggtg gtcttgccca cgccggtggg
    26521 tcccaccagg gcgtacacgc cgccccggtc gatgatgtcg gcgtcgctgg acatggcgcg
    26581 caggcgctgg ctcagcactg tgcgcaggat gcgatgggcg tcctcgacgg acgcgtcctc
    26641 gggcacggcg tcggccagct cccgcgccag cgcggcagaa aagccggcct ccagcaggtc
    26701 gctcatcagg cgggtacggg acggcgcggt gcgattgagc tcgccccacg caaagccggc
    26761 cagctggcgc tcgatcatgc cgcggatggc ggcgagttcg gtggccatct ggctgttggc
    26821 ggcctccagg gcgcggatgc gggcttcctc ttcgatgccg gtggcgcgcg gacggggcgc
    26881 cgcctcgcgg ggagcggccg gatcgccgcg caggggcgag cgtaccggct cggcgggctt
    26941 gcgggcgggt gcggcggcgt ggaaaacatc gcggccgagc tccactcgcg gcggattgaa
    27001 gggctgcatc tcgggcgcac ggggtgcggt cgcggccacg ggggccggac ggcggggcgc
    27061 ggcggctggc gccgctgcgg gctgggtacg ggccgcggcg gcgcgggaca gactcaggcg
    27121 gaaatcgtcg tcgaagaagt cggccgccgg ctgggacgcc cgggccgccg cacggggcac
    27181 ctcggtcgaa gccgcagcgg gtatcaccgg ggcagcaggc gccgcggccg ccgcctgctg
    27241 gcgcgcgcgc tgcagggccg ccacgtcgtt ggccggcagc gccagaatct ccaccccgcc
    27301 attcaccgcg cgattggaca gcacgacggc gtcgggcccc agctcttcct tgagttggcg
    27361 cagcgcctcg cgggcggttt tggcgaagta gcgcttgacg ttcatgctcc ggtctccggg
    27421 gtgcgatggc tcacaaggca gggatgcgat ccatttccat gcctccgatt atcgggagac
    27481 gggcgccatt cgatggccgg gaaaagacag gttttagggc gctattcccg tgttgggcgt
    27541 accggacggc gttcagcggg ccacggtgat catggaggcc acgcgaatca ggcgggtctc
    27601 cggcacctcg gcattggaga cgaccttgag ggtcgggacg ctgcggcgca ggaagcgcga
    27661 cagcagccag cgcagggccg gtggcaccag cagcaccggc ggcaggccga tgtcctccat
    27721 gcgctgggcg gcgacggcgg tctcccgcag cagggtgtcg gcgaggcccg gctcgatggc
    27781 gccgccctcc ggcccgccgc cgccaatggc ctgcaggagc acccgctcga tgcctggatc
    27841 gagggccatg acctggattt cgccgttgcc cgggaacagt ccctgcacga tggcgcggcc
    27901 aagggcctgg cggacgcggg tggtgagctc caccgggtcc tgcacgcgcg gcgcgcactc
    27961 ggccagcacc tcgacgatgg agcgcatgtc gcggatgttg accccttcgt cgaggaggtt
    28021 ctgcaagacc cgctggacgg tggcgagggg cagcagcttg ggcaccaggt cttccaccag
    28081 cttggggttg tccttggcga tgtggtccag cagggcctgg gtctcggtgc gccccaggag
    28141 ctcggcggcg tggctgagga ccaggtgatt gagatgggtc gccaccacgg tactggcgtc
    28201 cacgaccgtg tagccgaggg cgtgggcctg ctcccgctga ccggcatcca cccacaccgc
    28261 aggcaggccg aaggccggat cggtggtctc gcgaccgttg agggggccac tcacgcggcc
    28321 cggattgatg gccatgaact gtcccggaaa ggccatgccg ttgcccacct cgacgccctt
    28381 gaggacgatg cggtaggcgt tgggcttgag ctccaggttg tcgcggatgt gcaccgcaga
    28441 cgacaggaag ccgatgtccc gcgcgaactt cttgcgcagg ccgcggatac gcttgaggag
    28501 ctcgccatcc tgggccttgt ccaccatggg aatcaggcga tagcccacct ccagacccag
    28561 gacgtccacc ggcgtcacat cggtccagct ggcctcctgg gcctcgggcg gcacggccgg
    28621 cgcggcggcg gccggcacct cctcttcggc aatcgccgcc tgacgcttgc cgccgtacca
    28681 ggcgagcgca ccgagcccgc cggcgagcag caggaacacg aagttgggca tgcccggaat
    28741 gaggcccagc acgccgagga tggcggcggt gaggaccagc acctgggggt tggaaaagac
    28801 ctggccgacc acctggctgc ccatgtcccc ttcgtcgccc acccgggaca ccaccagacc
    28861 cgccgccacc gagatgatga gggccggcag ctgggcgacg aggccgtcac cgatggtcag
    28921 cagggtgtaa ttgttggccg cctgccccag ctccatgttg tgctggatga cgccgacgat
    28981 gagcccaccc accacgttga tgcccatgat caggatgccc gccaccgcgt cgccgcggac
    29041 gaacttggag gcaccgtcca tggcgccgta gaagtcggcc tcctgggcgg tctcggcacg
    29101 gcggcgcttg gcctcttttt cgtcgatgag gcccgcgttg aggtcggcgt cgatggccat
    29161 ctgcttgccg ggcatggcgt ccagggtga
//
LOCUS       DAOQMM010000038        29018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_456_length_29018_cov_50.761454, whole genome shotgun sequence.
ACCESSION   DAOQMM010000038 DAOQMM010000000
VERSION     DAOQMM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29018
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_456_length_29018_cov_50.761454"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLH85_08340"
     CDS             complement(<1..1009)
                     /locus_tag="PLH85_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB64611.1"
                     /translation="MSVALRLAPVFLGALARRRMTTLLSCAAIALGVALGMGVQVIHE
                     AALAEFGRGLRSLAGAADLQVVGPRDGFDEALYGVLAARPEVAAANPLVEVDVRPAGR
                     DETLAVLGVDVFAAAEVAPALMPQADGGAGRLGVLAEDAVFLSAAAAEAWGAGATLTV
                     QAGAATLSLRVAGSVPGVGAGRRLAVMDIAAAQRLFGRLGRLSRIGLRLAEGVSPAQA
                     QRALAPLLPAGVILEVPAAEETRSASLSRSYRVNLALLAAIALLTGGFLVFSAQALSV
                     VRRRREFAFLRALGLSRRSLMAWLVGEGAIIGGVGGVFGVALGLGLAWMAVQALGGDL
                     GA"
     gene            complement(1006..1659)
                     /locus_tag="PLH85_08345"
     CDS             complement(1006..1659)
                     /locus_tag="PLH85_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB64612.1"
                     /translation="MLEIDHLCRSFQGRSVLRDVCLRLQPGDYVAIVGASGVGKSTLL
                     NLIAGLDRPDGGRLSLDGVAYDALDEDALTRLRRARFGFVFQAFHILPHLTLRQNVAL
                     PLWLQGRGGAEADAAAWALLEEVGLGARADGWPRELSGGELQRVAIARALVHRPRLVL
                     ADEPTGNLDPDNGARVLSLLAERVRASGAIGVLVTHSAEAAASADRVLRLTPDGLTG"
     gene            1765..2586
                     /gene="hpnC"
                     /locus_tag="PLH85_08350"
     CDS             1765..2586
                     /gene="hpnC"
                     /locus_tag="PLH85_08350"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNB64613.1"
                     /translation="MPVDHYENFPVASFLLPARLREPVEAIYAFARSADDIADEGDAL
                     PVMRLARLNDYRRALHAIEQGTPYDDPGLAPMFDRLARAIRQFGLPVGLFRDLLDAFS
                     QDVGKTRYADFAELADYCRRSANPVGRLLLCLYRADTPENLRRSDWICTSLQLINFWQ
                     DVAVDLKKGRVYLPQDEMSLFGVCDEDLARGVVTPAVASLLDFQVTRARRMMTDGAPL
                     ARALPGRIGWELRLVIQGGLAILKKIEDVRYDVFRQRPTVGRTDWLRLAWRAAFR"
     gene            2592..3428
                     /gene="hpnD"
                     /locus_tag="PLH85_08355"
     CDS             2592..3428
                     /gene="hpnD"
                     /locus_tag="PLH85_08355"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNB64614.1"
                     /translation="MTDFHQYCQDKAAASGSSFYYSFLFLPPERRRAITALYAFCREV
                     DDVVDECNDPQVAQAKLDWWREEVHRVFDGAPHHPVGLALKDIASHFNLPREQLLEII
                     DGMQMDLVQTRYLDWKALHLYCYRVASVVGLLSAEIFGYTERQTLKYAHDLGLAFQLT
                     NIIRDVGEDARRGRIYLPVDELQRFNVPAHEILEARYSENFRSLMAFQAQRARGLYEQ
                     AFAQLPAADRKAQRPGLIMAAIYRSLLDEIEADGFQVLDRRTSLTPLRKIWIAGRTWL
                     RG"
     gene            3425..4735
                     /gene="hpnE"
                     /locus_tag="PLH85_08360"
     CDS             3425..4735
                     /gene="hpnE"
                     /locus_tag="PLH85_08360"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HNB64615.1"
                     /translation="MTPSVAIVGGGYAGFAAAVTLARGGARVTLFEASRVLGGRARVV
                     ETDGQRVDNGQHILLGAYSETLRLLRLVGVRPSVLATRRLALVYPGHCELRAAALPAP
                     LHLALGLLRARGLGTADKLAMLRLMRHLKRQRFRVAPDRSVGTLLTETAQTERLVRLV
                     WEPLCVAALNTPVADASAQVFAHVLRDSLAGSASASDLLLPRVDLSELFPVPASRWLA
                     LRGHTVRIVEPVKAIQRDDTGFRLDGDPFPTCYDHVVIATAPYHVAALVTGLPGMRPV
                     VTQIERLRHEPIVTAWLGFDGPVSFPEPMIGLSGGYGQWAFDRTALGGPTGLVSIVIS
                     ASGLHQQLDRDALRDALVAELAAALGPLPRLAWSRLITEKRATFACTPGLERPTPRTP
                     EPGLWLAGDYVASDYPATLESAVRSGVAAGRAILNACGLKESLF"
     gene            complement(4754..5458)
                     /locus_tag="PLH85_08365"
     CDS             complement(4754..5458)
                     /locus_tag="PLH85_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB64616.1"
                     /translation="MLTRTVPLCASSASVVVEVAALSRRVVSEGAALTILDDVSFSIA
                     AGEAVAIVGASGSGKSTLLGLLAGLDVPDSGHVRVRGQDLFALDEDGRAALRGASIGF
                     VFQSFQLLPALTALENVMLPLELAGLPDARAAAKGWLERVGLGGRLGHYPRQLSGGEQ
                     QRVAVARAFAPQPALVLADEPTGNLDAATGEAVIELMFALNREAGTTLVLVTHDTALA
                     ARCGRVLRLAAGRLVS"
     gene            complement(5931..7391)
                     /gene="guaB"
                     /locus_tag="PLH85_08370"
     CDS             complement(5931..7391)
                     /gene="guaB"
                     /locus_tag="PLH85_08370"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNB64617.1"
                     /translation="MRVIQKALTFDDVLLIPAHSSVLPRDVSLRTRLTRNVCLNIPLA
                     SAAMDTVTEARLAIALAQEGGIGIVHKNLTPQQQAAEVAKVKRFESGILKDPIIIPPT
                     MTVREVLTLTRTHKFSGLPVVDNGKVIGIVTNRDTRFETNLDQPVSAIMTPQARLVTV
                     REGASLDEARALMHKHRLERVLVLNEAGGLAGLITVKDMMKSTEHPLAAKDDLGRLRV
                     GAAIGVGAGTEERAALLAEAGVDVIVVDTAHGHAQGVLDRVNWVKKNFPKVEVIGGNI
                     ATAAAALALVEAGADGVKVGIGPGSICTTRIVAGVGVPQITAVDNVARALEGSGVPLI
                     ADGGIRFSGDISKAIAAGANVVMLGGLFAGTEEAPGETVLFQGRSYKSYRGMGSLGAM
                     QQGAADRYFQENDGNADKLVPEGIEGRVPYKGAVTAVIHQLMGGLRASMGYVGCATVD
                     EMRTRAEFVEITSAGMRESHVHDVQITKEAPNYHVD"
     gene            7475..7933
                     /locus_tag="PLH85_08375"
     CDS             7475..7933
                     /locus_tag="PLH85_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNB64618.1"
                     /translation="MSRALLALLILGASPLVLAQSIYSWKDAAGITHYSDTPPADSTS
                     RRLKQAPLAPPPTAQDGATQSFADKELAFRKRRAEAAEAEDKARKDKARDEDRQRECA
                     AAKSQLAGLESGQRIARFNAAGEKEYLDDAQRDAEIERARRVVENNCKPQ"
     gene            complement(8226..8555)
                     /locus_tag="PLH85_08380"
     CDS             complement(8226..8555)
                     /locus_tag="PLH85_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNB64619.1"
                     /translation="MSDSINVEVCYALQDRQEIVSLRLPAGSTVQKAVEASGLLQRHP
                     EIDLVKANKLGVFAKLVKGDTVLRDKDRVEIYRPLIADPKEVRRKRAEEGKVMKKGGG
                     SADEAAA"
     gene            complement(8557..8994)
                     /locus_tag="PLH85_08385"
     CDS             complement(8557..8994)
                     /locus_tag="PLH85_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNB64620.1"
                     /translation="MAAVKKIVLIEFTPAQMFDLVDRCEDYPRFLPWCGGSEVHHRDE
                     RITRATLHINYHGIKSHFSTENDKQYPTHMHIRLTEGPFTHLDGSWDFTPLGDAACKV
                     EFNLRYEFSSRLIEKAVGPVFNHIANTFVDSFVKRAGQIYGGK"
     gene            9062..9508
                     /gene="smpB"
                     /locus_tag="PLH85_08390"
     CDS             9062..9508
                     /gene="smpB"
                     /locus_tag="PLH85_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNB64621.1"
                     /translation="MSIIDNRKAYHDYFIEDKYEAGLVLEGWEVKAIRAGRSNIKESY
                     VVIRNGEIFIVGMHITALESASTHIHPDPTRTRKLLLHATEIDKLIGKVERAGYALVP
                     LDLHYTKGRIKALIGLAKGKKQFDKREAEKERDWEREKARLIRAKG"
     gene            complement(9545..10477)
                     /locus_tag="PLH85_08395"
     CDS             complement(9545..10477)
                     /locus_tag="PLH85_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64622.1"
                     /translation="MHLNLSFDLSAAVRRVRRPASVADASAIGAKVWKSFPYGNRLYR
                     YDGAALQANWARLHRGDREPFPSATRIERLVRHNPALAEHVRDPARTAACLEQAWRAY
                     HAGDFAEAVHFAQDAGVAGTMVAVRAAAVYAAHVEDQDDRRLKILREATEAAEELVQV
                     APVWANAWYAFGLAVGRYSQNLSVMKALTLGFGGKVRDSFLRALALEPDHADAHLALG
                     VYHAEVVDKVGSVVGALSHHARRDAVEHHFELARRLTPDSPIVLAEYAKARMLAFGAA
                     QFGRAYGLFQEAAACRPVDALERLDVELAKRELI"
     gene            complement(10679..10969)
                     /locus_tag="PLH85_08400"
     CDS             complement(10679..10969)
                     /locus_tag="PLH85_08400"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNB64623.1"
                     /translation="MNRNRLDAVQIDAALAELPGWQRVEGRDALGKTYRFADFNAAFG
                     WMARVALMAERLDHHPEWANVYSRVDVVLATHDAGGVTAFDVALARFMESAV"
     gene            complement(10989..14666)
                     /locus_tag="PLH85_08405"
     CDS             complement(10989..14666)
                     /locus_tag="PLH85_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNB64624.1"
                     /translation="MWQKVHWNGPWARGAKWGGGVLAVVGVVGFLVAPPVVRSVAESA
                     LTQALHRPVSIEGVSINPYALSAEVRGLRIDEPGGGVEAASFKALYANLEAESLFRGG
                     VVVKEVQLIEPHVSVIRTEGQRYNWSDLIDEILNKPDDGSKTFFSVHNIRVEHGRVDF
                     DDRPTGQKHTLADLNLGVPFVSNLPSQVETYVEPLLAVTLDGAPFEVRGKARPFSKAR
                     ESAVELRFEGVDLTPYLAYVPLEKPFRVPSARLSSNLVVTFAQPDGQAPDVAVKGEVG
                     LDAFELQDTAGHPAVKLPHVAVGIERLAPLAGVVSLGTVTVERPEVVVDRDRDGRLGL
                     LKLVPKPAPSSEPPSPAAAPSDTPPARPLAVTLGEFRLTGGKVMISDALPGGTYRRTL
                     DDLHVSLRKFDLAGAEPAALDAGFHTDLGESVEHVGTVGLAPLRAEGTITVDRLDLPG
                     HAAYYAAALKQADVVSGHFDGKLAYAFSIDKEEPRVSLKGEVLGLKDLVVRLKGEKAP
                     LAKVGTLELRELAVDTAARKVDVGEVIGKATRLALIRDGEGRFNAERMTATEAVPPPA
                     PEKPVRAAASKAPKAVAAGPAWQVAVRRVTLDDWGVRIEDQTLSPAIVFNAEPLSLRV
                     EGLSTAKGSKAKVELKASINKRGRFGVAGTVGLAPLAAALDLDLSAVDLAMLQPYVTE
                     KVNIAITRGVVTSKGRLAFELPDGGAMKGGFKGSLTIGDFASVDKLNAADFLKWRSLY
                     FGGVDVRLAPLAVNVDEIALTNFYTRLILDEQGGLNIREITAERARDQKRAKDAARAA
                     GKGSNAGSSAPPAPVAAKAVAAPAAPTPPPPVTIRRITLQGGNIAYSDRFVKPNYDAN
                     LTGMGGVLSGLSSDPSTLAELDLRGRVDNAAPVEVSGRLNPFRQDKALDIRASVTDFE
                     LSGVSTYAAKYVGYGIEKGKLSAKLNYKVEDRKLTASNQVFLDQLTFGDQVDSPDALK
                     LPVQLAVSLLKNSRGEIDLDLPISGTLDDPQFSVGGIIVKVIVNLIGKALTSPFALLG
                     SLFGGNAEELAWLEFDPGFARIDEKGESKLKAIAKAMSDRPGIKLEISGRVDPDADRD
                     GLRRAAMLGRVEALKVKEMARKGEAAGEDGRVRVDPAEYPVLLARVYKDADFVKPRTM
                     LGLTKDLPVPEMEKLLIANAKVTDEDLLTLAHQRAQVVKSWLIEQGKVAGERVFILAP
                     RVGKGGKDVSATLSRVDFSMR"
     gene            complement(14857..16923)
                     /locus_tag="PLH85_08410"
                     /pseudo
     CDS             complement(14857..16923)
                     /locus_tag="PLH85_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            17295..17732
                     /gene="pedF"
                     /locus_tag="PLH85_08415"
     CDS             17295..17732
                     /gene="pedF"
                     /locus_tag="PLH85_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HNB64625.1"
                     /translation="MIQVTVRKSSLFRLATAAALSLAAGALFAHGDVTPQAVDTSTLK
                     PVGKEWLAKNPYSKDKEAIRIGSSAFNQNCARCHGLGAVSGGIAPDLRYLPEGQEGDE
                     IFLNRIRHGATRDGRVYMPPFEGILSQEAMWTIRAWLETVRAD"
     gene            17722..18570
                     /locus_tag="PLH85_08420"
     CDS             17722..18570
                     /locus_tag="PLH85_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNB64626.1"
                     /translation="MPTDRRQFLTAAGACLLAAGLPARADGLEDIRRRGSLTVAVYND
                     FAPFSSKGKGIDVELGEALGRKLGLATQVIGYNADEDMDDDLRNMVWKGHYLGARPSD
                     VMMHVPVDSYLQGKNDKVRIFGPYHLETIAVARDPARVGAFRGSAANALELFTREKIA
                     VEGRSLSDAFLLGVLNGRLRDNVVHCKDIAEAIEKLKAGEVIAVMGPRSEIEVALGKG
                     TRFIVDIVQMPELKINNWPLGMAVKADEAPLADALAGALKALEQDGTVAEIFARHGVT
                     YLKPTV"
     gene            18578..19519
                     /locus_tag="PLH85_08425"
     CDS             18578..19519
                     /locus_tag="PLH85_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNB64627.1"
                     /translation="MRLTLSGRLLLACTAFPGLVLAAPFAYVPNEKSATVSVIDTATD
                     SVVDTLPGGKRPRGIATDGQRLYISEAPSSSLVVVDLARRQLLRSIRLGESPEGVSVS
                     TDGRLIAAAVEESNSVSLIQAATGKVLADIKVQGKNPEHAVFSPDGQWLLVSAEEAEQ
                     VDLIDVAARKQVASIAVGRRPRGIGFLPDSRRAYVACELDSTVYALDVTTRQATATIK
                     TGTFSNGIAVAPDGSRVYVSNGKDGTVSAIRTADNQVEATIPVGKRPWNMALTPDGRK
                     LYVANGKSNSVSVIDTTRLENIGDVPVGELPWGVVIR"
     gene            complement(19683..19997)
                     /locus_tag="PLH85_08430"
     CDS             complement(19683..19997)
                     /locus_tag="PLH85_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB64628.1"
                     /translation="MKFVQTVCAIALGCAVVSPALASEAIIKKARCVACHSVEEKRVG
                     PAYKDVAAKYKGQKDAEAALIAKVRNGGSGNWGQIPMPPHGPDKISDADLKAAIEWIL
                     KL"
     gene            20430..22295
                     /locus_tag="PLH85_08435"
     CDS             20430..22295
                     /locus_tag="PLH85_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNB64629.1"
                     /translation="MSPIDQHLINLPVDTIRRSWERCATRGLRADERLPDDPNARVRL
                     SDRLEANARLVAYAQPIMEHLHQQIARSSSTILLADDSGVILRSVGDSDFLERAARVA
                     LKPGHCWSEHVMGTNAIGTALTEQRPVAVIGEEHFLDRNKFLTCIAAPIHAPTGGVLG
                     ILDISCSARLTQVHALALLQTTTEIIENRLIETLDDASVVLRFHHQPEALASPLEGLA
                     VFNESGGMLACNRRAERLLGLPDTRHSQSAFNDIFETRWNVLLDHALRSSFRPTKLRC
                     NRGREFAATVLVSNLRRVRPAGAVRDPRVLPRPRPRLTLEDMDLGDKAVGTAIRRARR
                     ILGLDIPLLIQGETGTGKEVFAQAFHNSGPRRDGPFVAINCAAIPGNLIEAELFGYTA
                     GAYTGARGEGARGKLREAHGGTLFLDEIGDMPLKLQAVLLRVLETRRVTPLGGGPEEE
                     IDLSLVCASHRSLKALCEVGEFRSDLYFRLSAMSLALPPLRERSDLEGMARRILQEES
                     PDRPIRLATSTLDLIRRHPWPGNIRQLKNALRLGVAMLGDDEETLGPDHLPQDLLDGD
                     DTVHPVPAAKSLRAAEVRLVEDTVQRHNGNISAAARELGITRTTLYRKLRQTSDD"
     gene            22357..22761
                     /locus_tag="PLH85_08440"
     CDS             22357..22761
                     /locus_tag="PLH85_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64630.1"
                     /translation="MKIRPTSRPRRPTLAALCLAPLCALALNACSGRSDLPERLRQEF
                     RPGLSIAEAREKLTAHGTTYSIRNTMECEGLVRNSPIAALQPPQGGLCIFGKIPVSKS
                     WFGVRSDVILQLVFSGDGVLQDGNFEEVTSAQ"
     gene            complement(22888..23268)
                     /locus_tag="PLH85_08445"
     CDS             complement(22888..23268)
                     /locus_tag="PLH85_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB64631.1"
                     /translation="MNALCKAALLAGLAAVATTASAAADDEMKALATKSGCLTCHAIE
                     SGTPGPNGMAPIGPAWQDVAKHYKGKPGADAFLTRVVLEGSNPYGSHWKDQVSGLAMP
                     PNKVAIKEADAKKLVKWILTLSPK"
     gene            complement(23315..24655)
                     /locus_tag="PLH85_08450"
     CDS             complement(23315..24655)
                     /locus_tag="PLH85_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB64632.1"
                     /translation="MKISGVPSMDLRWKLFGTVLGFALALLTAAAAWIGGALQRDATQ
                     EMAASARLVDAIRAASAAPGMRVDELERLLGAEPLRHVRLQIERSNLEHSVSDAPAGP
                     MDLLAAALLGERTVPEQRIPLADGVLVVRADAHAEIREILWDGAGLMLTLLVFSVAMA
                     VVAWLAAHRALKPVRELEAGLARIARDTGPVRLPEFELREFSRIGVAIERLAGDLARE
                     RADRQTLARRLVELQEAERRELARELHDEVGQALTAMGVRAAFIERHAGRADAVRLSA
                     CARDIRTESGRLVKLVGTVLARLRPRGLDDGAQVAAALDGLVRTWQHRAPEVAIEADL
                     PARLPALPPSAGLALYRTLQEALTNALRHSLADRVTLSLRADADAIDLVVSDNGGGRA
                     TDVAARGRGGLLGMRERAEMAGGRVWLSDGPDGGLSVRLSLPLHGGDRVPVPAV"
     gene            complement(24782..26548)
                     /locus_tag="PLH85_08455"
     CDS             complement(24782..26548)
                     /locus_tag="PLH85_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HNB64633.1"
                     /translation="MKKPFAMTVIAAAVMVAGAAHAKEVTDADILNDAKTPGDVVHFG
                     LGQQGQRYSALDQINTKTVKDLVPVWSFSFGGEKQRGQESQPVVFDGKMYVTASYSRL
                     FALDAKTGKKLWKYEHRLPEGIMPCCDVINRGAALYDNLVIFATLDAQLVALNKDTGK
                     VVWKDKIDDYAAGYSASAAPLIVKGLVITGVSGGEFGVQGRMEARDAKTGKMVWMRPT
                     VEGHMGYKWVNGEKVENGISGTTNATWTGDMWKTGGAATWNGATYDPETNLVFAGTGN
                     PAPWNSHLRPGDNLYSSATVAIDPDTGKIVWHYQTTPHDGWDFDGVNEFVSFDYKDPK
                     SGKVIKAGGKADRNGFFFVIDRTNGKLLNAFPFVKDITWAKGIDLATGRPIYNDENRP
                     GDPSAGTDGKKGSTVFAAPSFLGGKNQMQMAYNPKTGLFYVPANEWGMDIWNEPVSYK
                     RGAAYLGAGFTIKALHDDYIGALRAVDPSAGKIVWENKNFAPLWGGVLTTGGNLTFYG
                     TPEGYLKALDAKTGKEVWSFQTGSGVVAPPVTWTEGGEQFVAVVSGWGGAVPLWGGDV
                     ARRVNFLEQGGSVWVFKLHKSK"
     gene            27043..27816
                     /locus_tag="PLH85_08460"
     CDS             27043..27816
                     /locus_tag="PLH85_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HNB64634.1"
                     /translation="MPRIITALMFALVCHAALAAAPADPPDPLNSGRWADMRKEVLPA
                     DARTVFDERIRVIAPISAENPLNVPITVDASALDGVEEVRVFADYNPIVQIVRFEPGG
                     ALATLGFRVKLQQSTPIRAAARGADGLWHVGGSWVNTTGGGCTLPSTGSSSPEWQRRL
                     GEVSSRVWARKEGGERIRLRIVHPEDTGLAAGIPAFYIEDLRLTDDGGRPLMRVRGFE
                     PIAENPVFTVDIPAAHTGHGYRINGRDNNGNTIAAEIDK"
     gene            27813..28793
                     /locus_tag="PLH85_08465"
     CDS             27813..28793
                     /locus_tag="PLH85_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HNB64635.1"
                     /translation="MSGAAPMRLRVPRLLRAPAAALALGWTLLLGAADFDYALQPRRI
                     ADDTWVLTGRNEDFTLANGGNIVNTAFIVTADGVVVIDSGPSRRYGEQLRQAIAQVTD
                     RPIRLVLNTHHHPDHILGNQAFPADTLAALAGTADGIHTEGGAFNDNMYRLVGDWMAG
                     TEVVTPARRLPSGIQEIGGHRLEFIALDGHTGADLAVLDRSTGVLFAGDLVFHDRSPT
                     TPHARLPAWNAALDRLQASGFRVLVPGHGPVAGDAGPIRQTRAWLAWLDGTLRSAAED
                     GLDMNEVMALPIPAEFRRLAVVETEYRRSVGQLYPAYEQAALHRPRRSGP"
     gene            28790..>29018
                     /locus_tag="PLH85_08470"
     CDS             28790..>29018
                     /locus_tag="PLH85_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64636.1"
                     /translation="MMTPALPHDWPWQPAPALPRTLRLALTASIGVHVLALMGHYGLL
                     PNPSDSPAPLQVELRLPPPVAPEPVRAVAPPL"
BASE COUNT         4904 a        10591 c         8955 g         4568 t
ORIGIN      
        1 ctgcccccag atcacccccc agcgcctgca cagccatcca cgccagcccc agacccagcg
       61 ccacgccgaa cacgcccccc accccgccga tgatcgcgcc ttcgcccacc agccaggcca
      121 tcagactgcg ccgcgacagc ccaagcgccc gcaggaaggc aaactcccga cgccgccgca
      181 ccaccgacag tgcctgcgcc gaaaacacca ggaagccgcc cgtcagcagc gcaatcgccg
      241 ccagcagggc cagattcacc cggtaggacc gcgacaggga cgcgctgcgc gtttcctccg
      301 ctgcaggcac ttcgaggatc acccccgccg gcaggagggg cgcaagcgcg cgctgcgcct
      361 gcgcgggcga cacgccctcc gcaagcctca gaccgatccg gctgaggcgc cccagccggc
      421 cgaaaaggcg ctgtgccgcc gcgatgtcca tcaccgccag acgccggccg gcgcccaccc
      481 cgggcacgct gccagccacc cgcaagctca gggtggccgc acccgcctgt accgtcagcg
      541 tggccccggc gccccaggct tcggcggcgg ccgcactgag gaacaccgca tcctccgcca
      601 gcacgcccag gcgaccggcg ccgccgtccg cctggggcat cagggccggc gcgacctcgg
      661 ccgcggcaaa gacatccaca cccagcacag ccagcgtctc gtcccgcccg gccggacgca
      721 cgtccacctc caccagcgga ttcgctgcag ccacctcagg ccgggccgcc aggacgccgt
      781 acagcgcttc atcaaagccg tcccgcggac ccaccacctg cagatccgcc gccccggcaa
      841 ggctgcgcaa cccccgcccg aactccgcca gcgccgcctc gtggatcacc tggaccccca
      901 tgcccagggc cacgccaagg gcgatggccg cgcaggacag cagcgtcgtc attcgccgcc
      961 gggccagggc cccgagaaag accggcgcca ggcgcagggc cacactcatc ccgtcagccc
     1021 atccggagtc aggcgcaaca cccggtccgc gctggccgcg gcctccgcgg aatgggtcac
     1081 cagcacgccg atggcgcccg acgcgcgcac ccgctcggcc agcagggaca gcacccgcgc
     1141 cccgttgtcc ggatcgagat tgcccgtcgg ctcgtcggcc agcaccagcc gcggtcgatg
     1201 caccagcgcg cgggcgatgg ccacgcgctg cagctcaccg ccggacagtt cccgtggcca
     1261 gccgtccgcc cgcgccccca gacccacctc ctcgagcagt gcccacgccg ccgcatccgc
     1321 ttcggccccg ccgcgcccct gcaaccacag gggcagcgcc acgttctggc gcagggtgag
     1381 gtggggcagg atgtggaagg cctggaagac aaagccgaaa cgcgcccgcc gcaggcgtgt
     1441 cagggcatcc tcgtcaagcg catcgtaagc cacgccgtcc agggacaggc gccccccgtc
     1501 cggacgatcc agcccggcaa tgagattgag cagggtggac ttgccgacgc cggacgcccc
     1561 gacgatggcc acgtagtcgc cgggctgaag ccgcaggcac acatcccgca gcacgctgcg
     1621 cccctggaag ctgcggcaaa ggtgatcgat ctcgagcatg ctgacatgga cggcgggctt
     1681 gcccggccgg ttccggaaga cggaaaggca atggatgcgc gggcgcggag ccaagcgttg
     1741 cacagggtat actccgccgc gcccatgccc gtcgaccact acgagaattt tcccgtcgcc
     1801 tccttcctgc tgccggcccg cctgcgcgag ccggtggagg ccatctacgc cttcgcgcgc
     1861 agcgccgacg acatcgccga tgaaggcgac gccctgccgg tgatgcgcct ggcgcgcctc
     1921 aacgactacc gtcgcgccct gcatgccatc gagcagggca caccctacga cgatcccggc
     1981 ctggcaccga tgttcgaccg cctggcccgc gccatccgcc agttcggcct gcccgtcggc
     2041 ctcttccgcg acctcctcga cgccttcagc caggacgtgg gcaagacccg ctacgccgat
     2101 ttcgccgagc tggccgacta ctgccgccgc tccgccaacc cggtcggccg cctgctcctg
     2161 tgcctgtacc gtgccgacac acccgagaac ctgcgccgct ccgactggat ctgcaccagc
     2221 ctgcagctca tcaacttctg gcaggacgtg gccgtcgatc tcaagaaggg ccgcgtctac
     2281 ctgccccagg acgagatgag cctgtttggc gtgtgcgacg aagatctggc ccgcggcgtc
     2341 gtcacccccg cggtggcctc gctgctggat ttccaggtca cccgcgcccg ccgcatgatg
     2401 acggacggcg cccccctcgc ccgtgccctg cccggccgca tcggctggga actgcgcctg
     2461 gtgatccagg gcgggctggc catcctcaag aagatcgagg acgtccgcta cgatgtcttc
     2521 cggcaacgcc ccaccgtggg cagaaccgac tggctgcgcc ttgcgtggcg cgccgcattc
     2581 cgttgattcc catgaccgat ttccaccagt actgccagga caaggcggcc gccagcggct
     2641 ccagcttcta ctacagcttc ctcttcctgc cccccgagcg ccggcgcgcc atcacggccc
     2701 tgtacgcctt ctgccgcgag gtggacgacg tcgtggacga atgcaacgac ccccaggtgg
     2761 cccaggccaa gctcgactgg tggcgcgaag aagtgcaccg cgtcttcgac ggcgcccccc
     2821 atcacccggt cggcctggcg ctcaaggaca tcgcgtcgca cttcaacctg ccccgcgagc
     2881 agttgctcga gatcatcgac ggcatgcaga tggatctggt ccagacccgc tacctggact
     2941 ggaaggccct gcacctctac tgctaccggg tcgccagcgt ggtgggcctg ctgtccgccg
     3001 agatcttcgg ctacaccgaa cgccagaccc tcaagtacgc ccacgacctg ggcctcgcct
     3061 tccagctcac caacatcatc cgcgacgtgg gtgaggacgc ccgccgcggc cgcatctacc
     3121 tgcccgtcga cgagctgcag cgcttcaacg tcccggccca cgagatcctc gaagcgcgct
     3181 acagcgaaaa tttccgcagc ctgatggcct tccaggccca gcgcgcccgc ggcctctacg
     3241 agcaggcctt tgcccagctc ccggcggccg accgcaaggc ccagcgtccc ggactcatca
     3301 tggcagccat ctaccgcagc ctgctggacg agatcgaggc cgacggcttc caggtactcg
     3361 atcgccgcac ctccctcacg cccctgcgca agatctggat cgccgggcgc acctggctgc
     3421 gcggatgacg ccgtcggtcg ccattgtcgg cggcggttac gcgggattcg ccgccgccgt
     3481 caccctggcc cgtggcggcg cccgtgtcac cctcttcgaa gcctcccgcg tgctcggcgg
     3541 gcgggcacgg gtcgtggaga ctgacggcca gcgcgtggac aacggccagc acatcctcct
     3601 cggcgcctat tccgaaaccc tgcgcctgct gcgcctggtg ggcgtgcgac cgtccgtgct
     3661 cgccacccgc cgcctcgccc tggtgtatcc cggccactgc gaactgcgcg ccgcggccct
     3721 gcccgcaccg ctccacctgg cgctcggcct gctccgcgcc cgtggcctcg gcaccgcgga
     3781 caagctcgcc atgctgcgcc tgatgcgtca cctcaagcga cagcgcttcc gcgtggcgcc
     3841 agaccgctcg gtcggcaccc tgctcaccga aaccgcccag accgaacgcc tcgtccgcct
     3901 ggtctgggag cccctctgcg tggcggccct caacacgccg gtagcggatg cctccgccca
     3961 ggtctttgcc cacgtgctgc gggacagcct cgccggcagc gcatccgcgt cggacctgct
     4021 gctgccccgc gtggacctct ccgaactgtt cccggtgccc gcttcccgct ggctggccct
     4081 gcgcggccat acggtgcgca ttgtcgagcc ggtcaaggcc atccagcggg acgatacggg
     4141 cttccgcctc gacggcgacc ccttccccac ctgctacgac cacgtggtga tcgccaccgc
     4201 gccctaccac gtggccgcgc tggtgaccgg tctgcccggt atgcggccgg tggtcacgca
     4261 gatcgagcgc ctgcgccacg agcccatcgt gaccgcatgg ctgggctttg acggcccggt
     4321 gagtttcccc gaacccatga tcggcctcag cggcggctat ggccaatggg ccttcgaccg
     4381 taccgccctg ggcggcccga cggggctggt cagcatcgtc atcagcgcca gcggcctgca
     4441 ccagcagctc gaccgggacg ccttgcgcga cgcgctggtg gcggagctgg ccgccgccct
     4501 cggccctctg ccgcgcctcg cctggtcccg cctgatcacc gaaaagcgcg ccaccttcgc
     4561 gtgcacaccg ggtctggagc gtcccacccc gcgcacgcca gaacccggcc tgtggctggc
     4621 cggtgactac gtggcctcgg actacccggc gaccctggaa tccgccgtgc gcagcggcgt
     4681 cgccgccggg cgcgccatcc tgaacgcctg cgggctcaag gaaagcctgt tctgacccgg
     4741 ccgacggccg gcgtcacgac accagccgcc cggccgccag acgcagcacc cgtccgcagc
     4801 gggccgccag cgccgtgtcg tgggtcacca gcaccagggt cgtgcccgcc tcccggttca
     4861 gcgcgaacat cagctcgatg accgcctctc cggtcgccgc atccaggttg cccgtcggct
     4921 cgtccgccag caccagtgcc ggctgcggtg cgaaggcccg cgccacggcg acgcgctgct
     4981 gctcaccgcc ggacagctgg cgcggatagt gaccgagccg tccgcccagc ccgacccgtt
     5041 ccagccagcc cttcgccgcg gcccgcgcat cgggcaggcc ggccagttcc aggggcagca
     5101 tcacgttctc cagcgccgtc agcgccggca ggagttggaa ggactggaac acgaagccga
     5161 tgctggcccc acgcagcgcc gcgcggccat cctcatccag ggcgaacagg tcctgtccgc
     5221 gcacccgcac atggccgcta tccggcacgt ccaatcccgc cagcagaccc agcagggtgg
     5281 acttgccgga gccggacgcc ccgacgatgg ccaccgcctc gccagcggcg atggaaaacg
     5341 acacgtcgtc cagaatcgtc agcgcagcgc cctccgacac gacgcggcgg ctgagggcgg
     5401 cgacctccac gaccacggac gcggaagagg cgcaaagggg aacagttcgg gtcagcatgg
     5461 cgggatggag tgccggagga ggccggaggt tcagtccggc cgcacaaaga gacggattat
     5521 cgcactggta cgatgacgcg ccggtgtcgt cccgggacgc gttgcagcgc ccgcccggcg
     5581 ctcgcgcagg ccatccgtga tcggacccgc gaccgccagc ctcccgtgcg tctcccggcg
     5641 cccacaggtc gcgcccgccc ggacagcgca ccgccatcat gagcgcggcc acagcctggc
     5701 caggcgccgt cgtaccgtgg gcgtgtcgcc cggcgttgaa ggggcacggg tgcgccatgc
     5761 ccgcagcgcg gaatcatcgg cacgacattc accatccgcg ccgacaccgt ccctgcatgt
     5821 accggaaaat ctgcacccgc agcgctccac gatgcgcgcg tcaatggaaa agggcagccc
     5881 gcaggctgcc cttccgtgtc atgcggggcg ttccgcctgc gcggcagccc tcaatccacg
     5941 tggtagtttg gcgcttcctt ggtgatctgc acgtcgtgga cgtgggattc gcgcatgccc
     6001 gccgaggtga tctcgacgaa ttcggcacgg gtgcgcatct catccacggt cgcacacccc
     6061 acgtagccca tggacgcccg caggccgccc atcagctggt ggatcaccgc cgtcaccgcg
     6121 cccttgtagg gcacgcggcc ttcaataccc tcgggcacca gcttgtcggc gttgccgtcg
     6181 ttctcctgga agtaccggtc ggcggcgccc tgctgcatcg cccccaggga gcccatgccg
     6241 cggtaggact tgtagctgcg cccctggaac agcaccgttt cgcccggcgc ttcctcggtg
     6301 ccggcgaaca gtccacccag catcaccacg ttggcgccag ccgcaatggc cttggagatg
     6361 tcgccggaga agcggatgcc accgtcagcg atgaggggca caccggagcc ttcgagggcg
     6421 cgggccacgt tgtccaccgc ggtgatctgc ggcacgccca cgccggccac gatccgcgtc
     6481 gtgcagatgg agccggggcc gatacccacc ttgacgccgt cggcgccggc ctcgaccagc
     6541 gccagcgccg cggcagccgt ggcaatgttg ccgccgatga cctcgacctt ggggaaattc
     6601 ttcttgaccc agttcacccg gtccagcacc ccctgggcgt ggccgtgggc cgtatccacg
     6661 acgatcacgt ccacgccggc ctcggccagc agcgcggccc gctcttcagt accggccccc
     6721 acgccgatgg ccgctccgac ccgcagccga cccaggtcgt ccttggcggc gaggggatgc
     6781 tcggtggact tcatcatgtc cttgacggtg atcagccccg ccaggccgcc ggcctcattg
     6841 agcaccagca cccgctccag gcggtgcttg tgcatcagcg cgcgggcttc gtccaggctg
     6901 gcgccttcgc gcaccgtgac cagccgcgcc tgcggcgtca tgatggcgga gaccggctga
     6961 tccaggttcg tctcgaagcg ggtatcccga ttggtgacga tgccgatgac cttgccgttg
     7021 tccaccacgg gcagacccga gaacttgtgc gtgcgggtca gggtcaacac ttcgcggacc
     7081 gtcatggtcg gcggaatgat gatgggatcc ttgaggatgc ccgactcgaa gcgcttgacc
     7141 ttggccacct cggccgcctg ctgctgcgga gtgagattct tgtggacgat gccgattcca
     7201 ccttcctgcg ccagggcaat ggccagacgc gcttcggtga cggtgtccat ggcggcggac
     7261 gccaggggaa tattcagaca gacgttgcga gtcaggcggg tgcggaggct gacatcgcgc
     7321 ggaagaacac tggaatgggc ggggatgaga aggacgtcat cgaacgtcag cgccttctgg
     7381 atcactcgca tggctataat cccttcgacc aaatccgtat tatacgcagt cgccccttgc
     7441 cccgcaaaag ctttgtcctt ccggagtccc gttcatgtcc cgcgccctcc tcgccctcct
     7501 gatcctcggt gccagcccgc ttgtcctggc ccagagcatc tacagctgga aggatgctgc
     7561 aggcataacg cactactcgg atacgccgcc cgccgacagc acgtcgcgcc gcctgaagca
     7621 ggccccgctc gcgccgcccc cgactgcgca ggacggcgcg acacaaagct ttgccgacaa
     7681 ggaactcgcc ttccgcaagc gccgcgccga agccgccgaa gccgaggaca aggcgcgcaa
     7741 ggacaaggcc cgcgacgagg atcgccagcg tgagtgcgca gcggcaaaat ctcagctcgc
     7801 cgggctggag agcgggcaac ggatcgcccg cttcaatgca gcgggcgaaa aggaatatct
     7861 tgacgacgcc cagcgcgatg ccgaaattga acgcgcccgg cgtgtggttg aaaacaactg
     7921 caagccccaa tagcagcgaa ggtgcaggtt ggtcgtccct tcagcattca cctgacgggc
     7981 ggacccagcg gtcacgtacg gccaagatgg caacatcacg cgcccgaagt gacgggccct
     8041 caggcccgtc cgaatccttc gtggcgcacc cgaccaggac atctcatcca ccctgcccga
     8101 acggtgcgat gcacgtcgtc gtgcaccgtg cctgatcgtc acacggggca ggcgtaacga
     8161 gccttcccac ctcagacgca cccactccca ctccggggca agggcctccc gagcgtgttc
     8221 gatgctcagg ccgccgcctc gtcggcggag ccgcccccct tcttcatcac cttcccctct
     8281 tcggcgcgct tgcggcgcac ctccttcggg tcggcgatga ggggtcggta gatctccacg
     8341 cgatccttgt cgcgcaacac cgtgtcccct ttgaccagct tggcgaacac gcccagcttg
     8401 ttggccttga ccagatcgat ctcgggatgg cgctggagca ggcccgacgc ctcgacagcc
     8461 ttctgcacgg tgctgcccgc cggcagcctg aggctgacaa tctcctgccg atcctgcagg
     8521 gcgtagcaga cttccacgtt gatcgaatcg gacatcctac tttccaccgt aaatctgacc
     8581 ggcacgcttc acgaaggaat cgacgaaggt gttggcaatg tgattgaaaa ccggacccac
     8641 cgccttttcg atgagccggc tggaaaattc atagcgcagg ttgaactcca ccttgcacgc
     8701 agcatcgccc agcggcgtga aatcccagct gccatccaga tgcgtgaacg gcccctcggt
     8761 cagacggatg tgcatgtggg tggggtactg cttgtcattc tcggtgctga aatgggattt
     8821 gatgccgtgg tagttgatgt ggagcgtggc gcgggtgatc cgttcgtccc ggtgatgcac
     8881 ctcgcttccg ccacaccagg gcagaaacct gggatagtcc tcgcagcgat ccaccagatc
     8941 gaacatctgc gcaggggtga attcgatcag gacgattttc ttgacagcgg ccatcggtcg
     9001 cgagccttgc gctcgaactg gtaaaatacg cgattttacg catccgttcg cgccgccgcc
     9061 aatgagcatc atcgacaatc gcaaggcgta ccacgactat ttcatcgagg acaagtacga
     9121 agccggactg gtcctcgagg gctgggaggt gaaagccatc cgcgccggac ggtcgaacat
     9181 caaggaaagc tatgtggtga tccgcaacgg cgagatcttc atcgtcggca tgcacatcac
     9241 ggccctcgaa tccgcgtcca cccatatcca tccggacccg acccggaccc gcaagctgct
     9301 gctccatgcc accgagatcg acaagctgat cggcaaggtg gaacgcgctg gctatgccct
     9361 ggtgcccctt gacctgcact acaccaaggg ccgcatcaag gcattgatcg ggctcgccaa
     9421 gggcaagaag cagttcgaca agcgggaagc cgaaaaggaa cgcgactggg agcgcgagaa
     9481 ggcccgcctg atccgcgcca agggctgagg gaccgcccct cacccgccgg gcgcgggacc
     9541 ggcatcaaat caactcgcgc ttggccagtt ccacatcaag ccgctccagg gcgtccacgg
     9601 gccgacaggc cgccgcctcc tgaaacaggc cataggcacg cccgaactgg gcggccccga
     9661 aggccagcat gcgcgccttg gcgtattccg ccagcacaat gggtgagtcc ggcgtgagcc
     9721 ggcgggccag ctcgaagtgg tgttcgacgg cgtcacgccg ggcatggtgg gacagggccc
     9781 ccaccacgga tccgaccttg tccaccacct ctgcgtgata aacgcccagc gccagatgag
     9841 cgtcggcatg gtccggctcg agtgccagcg cccgcaggaa actgtcgcgc accttgccac
     9901 cgaaccccag ggtgagagcc ttcatcacgg agaggttctg gctgtagcgc cccaccgcca
     9961 gcccgaacgc ataccacgcg ttggcccaca ccggcgccac ctggaccagt tcctcggccg
    10021 cctccgtagc ctcccgcagg attttcagac gccggtcgtc ctggtcttcc acatgggctg
    10081 cgtagaccgc tgccgcccgc accgccacca tggtaccggc cactccggcg tcctgcgcga
    10141 agtgcaccgc ctcggcgaaa tcccccgcgt gataggcccg ccaggcctgc tcgagacatg
    10201 cagccgtccg cgccgggtcg cgcacatgct cagccaatgc ggggttgtgg cggaccaggc
    10261 gttcgatccg tgttgcgctg ggaaacggct cccgatcacc ccgatgcaga cgcgcccagt
    10321 tcgcctggag cgccgcgccg tcatagcggt acaggcgatt gccgtaagga aaggacttcc
    10381 agacctttgc cccgatcgcg gacgcgtccg ccaccgacgc agggcggcgg acacggcgga
    10441 ccgcagcgct caggtcgaaa ctgagattca gatgcatggc agctcccccg gggatccaca
    10501 ttgttcttga tcattgtctc ggggcgtcct gcgccttccc gtggggaggg gctgaggaac
    10561 ccggacagat gtgttccgcg tcgtgcgcgg aggcgtggtc ggatcggggc gtagcgtacc
    10621 accaccggcg ccgcgaagca tgtcgcagcg ccgcattcgg gcgaaaaggc gtgcacacct
    10681 acacagcaga ctccatgaag cgagccaggg ccacatcgaa cgccgtgacg ccgcccgcgt
    10741 cgtgggtcgc aagcaccaca tccacccggc tgtagacgtt cgcccactcg ggatgatggt
    10801 caagcctctc ggccatcagc gccacccgtg ccatccagcc gaaggccgcg ttgaagtccg
    10861 caaagcggta ggtcttgccg agtgcgtcgc ggccctcgac acgctgccat cccggcagtt
    10921 ccgccagggc ggcgtcgatc tgtacggcat ccagtcggtt ccggttcatc ggacagcctc
    10981 ccaaaggttc agcgcatgga gaaatcgaca cggctcaggg tggcgctgac gtccttaccg
    11041 cccttgccga cccgcggagc aagaatgaag acacgttcgc cggccacctt gccctgttcg
    11101 atcagccagc tcttcacgac ctgagcccgc tgatgggcca gggtgagcag atcctcgtcg
    11161 gtgaccttgg cgttggcgat gagcagcttt tccatctcgg gcaccggcag gtccttggtg
    11221 agccccagca tggtccgcgg cttgacgaaa tccgcgtctt tatagacccg cgccagcagg
    11281 accgggtatt cagcggggtc aacgcgcacg cgcccatcct cacccgccgc ctcgcccttc
    11341 ctggccatct ccttgacctt gagtgcctcg accctgccca gcatcgctgc gcggcgcagg
    11401 ccgtcccggt cggcatccgg atcgacacgt ccggaaatct cgagcttgat gccgggccgg
    11461 tcggacatgg ccttggcgat cgctttcaac ttcgactctc ctttttcgtc gattcgcgca
    11521 aaaccgggat cgaactcgag ccacgcgagt tcttcagcgt tcccgccaaa cagcgagccc
    11581 agcaaggcaa agggggaggt caaggccttg ccgatgagat tgacgatgac cttgacgatg
    11641 atgccgccga cactgaactg gggatcgtcc agcgtgccgc tgatgggcag atccaggtcg
    11701 atctcgcccc ggctgttctt gagcagggac acggccagct gcaccggcag cttgagcgca
    11761 tccgggctgt ccacctggtc accgaaggtc agttgatcga ggaagacctg gttgctggcc
    11821 gtgagcttgc ggtcttccac cttgtagttc agcttggccg acagcttgcc tttttcgatg
    11881 ccatagccca cgtatttggc cgcgtaggtg gacacgccgg acagctcaaa atccgtcacc
    11941 gacgcacgga tatcgagggc cttgtcctga cggaagggat tcagccggcc cgacacttcg
    12001 accggcgccg cgttgtccac ccggccccgc aggtccagtt cggccagggt gctcgggtcg
    12061 gacgacagcc cggacagcac gccgcccatc cccgtcaggt tggcgtcata gttcggtttc
    12121 acgaagcggt cgctgtaggc gatattgccg ccctgcaggg tgatccgcct gatcgtgacc
    12181 ggaggcggcg gagtgggtgc agcgggcgcc gccacggcct ttgctgcgac cggcgcgggc
    12241 ggcgcactgc tccccgcatt gctccccttt ccggcggcgc gggcggcgtc ctttgcccgc
    12301 ttctgatccc gcgcccgctc ggcggtgatt tcgcggatgt tcaggccacc ttgttcgtcc
    12361 aggatcaggc gggtgtagaa attggtcagc gcgatctcgt cgacattgac ggccaggggc
    12421 gcaagacgga catccacgcc gccgaagtag agcgagcgcc acttgaggaa atcggcggca
    12481 ttgagcttgt ccaccgaagc gaaatccccg atggtcaggc tgcccttgaa ccctcccttc
    12541 atggcgcccc catcgggcag ctcgaaggcg agacgtccct tcgacgtcac cacaccgcgg
    12601 gtgatggcga tattcacctt ctccgtcacg tagggttgga gcatcgccag gtccaccgcc
    12661 gacagatcca ggtccagggc cgcagccagc ggcgccagcc ccaccgtgcc tgccacgccg
    12721 aagcgcccgc gcttgttgat ggaggccttc agctccacct ttgccttgct gcccttggcg
    12781 gtcgacagcc cttcgacacg cagggaaagg ggctcggcat tgaagacgat ggccggggac
    12841 agggtctggt cctcgatgcg cacgccccag tcatccagag tcacacgcct gaccgccacc
    12901 tgccatgccg gaccggcggc cacggctttc ggggccttgg acgctgcagc cctgaccggc
    12961 ttctccgggg caggcggagg caccgcctcg gtggccgtca tccgttcggc gttgaaccgc
    13021 ccctccccgt cgcggatcag ggccagccgc gtcgccttgc cgatcacttc gcccacatcc
    13081 accttgcggg ccgccgtatc cacggccagt tcgcgcagtt ccagcgtccc caccttcgcg
    13141 aggggcgcct tctccccctt caggcggacc accaggtcct tcaggcccag gacttcgcct
    13201 ttcaggctta cgcggggctc ctccttgtca atggagaacg cataagccag cttgccgtcg
    13261 aaatgacctg acacgacatc ggcctgcttc agggccgccg cgtaatacgc ggcatggccc
    13321 ggcaggtcga gtcggtccac cgtgatcgtg ccctcggcgc gaagcggtgc caggcccacg
    13381 gtgccgacat gctcgaccga ttcgcccaga tcggtgtgga agccggcgtc cagtgcagcg
    13441 ggctcggctc ccgccagatc gaacttgcgc aggctgacat gcagatcgtc cagcgtgcgc
    13501 cggtaggttc cgcccggcag cgcgtcggaa atcatcacct tgccgccggt cagtctgaat
    13561 tcgccgagcg tgacggcaag cggcctcgcc ggcggagtgt ccgacggggc tgccgccggc
    13621 gacgggggct ctgacgacgg ggcgggtttc gggacgagct tcagcagacc gagccggccg
    13681 tcccggtccc gatcgacgac gacttccggg cgttcgacgg tcactgttcc cagagacacg
    13741 acaccggcca ggggggcaag acgctcgatg cccaccgcga cgtgaggcag cttgaccgcc
    13801 ggatgtcccg ctgtgtcctg cagttcgaaa gcatcgagcc ccacctcacc cttcaccgcc
    13861 acgtccggcg cctggccgtc cggttgggcg aaagtcacca cgagattcga cgacagccgc
    13921 gcggacggca cacggaaggg cttctcgagg ggcacatagg ccagatacgg cgtgaggtcg
    13981 acgccttcaa acctgagctc gaccgccgat tcacgggctt tggaaaacgg ccgggccttg
    14041 ccccggacct cgaagggcgc gccatccagg gtcacggcga gcagcggctc gacataggtt
    14101 tccacctggg acggcagatt ggagacaaag ggtacgccca ggttcaggtc ggccagcgtg
    14161 tgcttctgcc ccgtgggacg gtcgtcaaaa tccacacggc catgctcgac ccgaatgtta
    14221 tggacggaga agaaggtctt ggatccatcg tccggcttgt tgaggatctc atcgatcagg
    14281 tccgaccagt tgtagcgctg cccctccgta cggatcacgc tcacgtgggg ctcgatcagc
    14341 tggacttcct ttaccaccac gccgccacgg aacagggact cggcctcaag gtttgcataa
    14401 agcgccttga aactggccgc ctcgacgcca ccgccgggct catcgatgcg caggccgcgc
    14461 acctcggcgg acaaggcata cggattgatc gacaccccct cgatgctcac cggtcggtgc
    14521 aaggcctggg tgagggccga ctccgccacc gagcgcacca ccggtggcgc caccagaaag
    14581 cccacaaccc cgacgacggc cagcaccccg ccgccccatt tcgctccccg ggcccagggc
    14641 ccgttccagt gcaccttctg ccacatcttg ttgttctcct tcagcgccgc gtcgacgcgc
    14701 agcggcgggc catcccgtcc tttgcaagcc tagcaggccc gacggggggt gagaatgaac
    14761 ttgtgcggac ggcgccggcc ggccaccgcc gggtgtggct taccggcaaa aaaaaagagg
    14821 cgcccgcagg cgcctcgcgt caaacggccg agccgctcaa aaacgtccca gcttgcgata
    14881 cagcgtattg cgactgatgc cgagtcgacg cgccgcggca gaaatgttgc ccttttccgc
    14941 ctccagcgtg cgctgaatca tttcccgttc gatctcttcc aggcggccga taccggctgc
    15001 gccaacgggc tcggccaccg ccatcggccg gacttcgctg accagccccg gcaccttgat
    15061 cggcgcagcg ggggcagccg cagccagctg accgatctcc tcaaagatgt cgtcgggcag
    15121 atggcagggg cggatgacgt cctcgtcgtc gtccagcagc gccagcgcca cgcggatgac
    15181 attgttgagc tgacggatgt tgcccggcca gttgtagcga cgaaaggcat ccatgacttc
    15241 ggccgacacc cgtaccgagc ggttcgggcc cgcttcgcaa cgcagcagct tgtcgatcag
    15301 acggtcgatg tcgctgcgct cccgcaggga cggcagattc acacagagtc cgttgagccg
    15361 gtagtacaga tcctcacgga atccgccccg cgccacttct tccttgagcc tgcggtgcgt
    15421 cgcgcagacc agggtgatgt ccaccgggat gctcttgttg gtgcccagcg gcgtcacaca
    15481 gcgttcctgc agtacgcgca gcagacgcgc ctgcaggggc agcggcatgt cgccgatttc
    15541 atcgaggaac agggtaccgc catgagcctg ctggatcttg ccgacagcgc cttccttgcg
    15601 tgcgccggtg aaggcaccgc ccgaatagcc gaagagctcg gattcgatca gggtctccgg
    15661 gatcgccgcg cagttgagcg ccacgaaggg cccctgggca cgaggtccgc tgttgtggaa
    15721 ggccttggcg aacatctcct taccggcacc ggactccccc tggatcagga tggggatgtc
    15781 ccggccgatc acccgcagcc ccttgtcgat ggccgcctgg aggcgttcgt cgccggtcgc
    15841 cagggattgc agcgtgagcc ggctcttgtc catggacgca cccgcgctga ccgaaaccgg
    15901 cgcgggggtc ggcgcagcag gcgcctcgac cgcggccttg agcgccttgc cgcggacatc
    15961 ggtgaggccc gtggacgcca ccggcattgc cgcaaagtta ccccgcagac gggcatagaa
    16021 gcgctcgcca ttacggccgg agagggcaat cagcgacagg gggtcgcgcc gcgcccggtc
    16081 caccagcgta cccaggggca cgtcgaagag catgccgtaa tgccggcgcg gcagatcctc
    16141 ccggcgcatg ccgaggagct cgagggcgac ctggtcggca cccagcaggt cgccgtcggg
    16201 ggtgatggcc agcagggcct cacagaggct gcctacgtga tcgggacggg aatggaaact
    16261 cagcaggccc tgatgggcga attcggcttc gaacaggcgt ttttcgataa tgcgggagga
    16321 catcttcacc agaccaaggg aatgacgctg gaaggcacgg tagtcgctgg agatatccag
    16381 cacacccatc aggcgcccgt gcggatcgag gatcggcgag gcactgcagg tcaggaaggc
    16441 gttgccttcg aagaagtgct ccgacccgaa cacgttcacc ggcacccgtt cggccagggc
    16501 tgttccgatg gcgttggtgc cgcgttcgaa ctcgtgccag ctggcaccgg gactgagggc
    16561 cacctgttcg gcacggctca ggaaatccgc gtcgccgtag gtctggagca ccatgccccg
    16621 ggcgtcggcc aggatggcca tgctgtggga attgcggatc tgctcgaaca ggtgttcgag
    16681 caccggctgg gcatgggaca tcaaggtacg gttgcgctac cgcgcttcca cgacgccccg
    16741 ctggttcggg tcgccgcatt cgaggcgtgc ggggttgagc ccggcctgcc ggcatcgttc
    16801 ccaggaacgc agcacactct gatcgaccag ggcttcaggg agttcgcccc gaccgaagta
    16861 gagtgaccgc gcctcctgga ggctgaccga gcgcgcctcc acactataag gaacctgaat
    16921 catctcctcc tccgttcaat acggattgtc ggcgccgact gacggatgac cgccgccggc
    16981 ttatgtatcg gcacgagagg aaggtgggtt aagccatggg cgctggccac actttccgat
    17041 ccctgcaccg agatgctcca aaatgtagca gctgaaccac attacagcaa cgtgactgct
    17101 ccacagtgca tccacctgag caagtgttgg gccagtttat tgcaccatca gaaaaactga
    17161 aaaatcctcg cctttccaga tccccctcca tcaaacattc cccggaggcg actggcacgc
    17221 cccttgctgt tgaggaacgc agacgcggaa cgccccggcg atccgcccga ccgactccaa
    17281 ctacacaacc aaaggtgatc caggtgaccg tccgtaaatc ctcccttttc cgtcttgcca
    17341 ccgccgctgc cctctccctc gctgccggcg ccctcttcgc ccacggtgac gtgacgcccc
    17401 aggccgtcga tacgtccacc ctcaagccgg tcggcaagga atggctcgcc aaaaacccgt
    17461 acagcaagga caaggaagcc atccgcatcg gctcctccgc cttcaaccag aactgcgccc
    17521 gctgccatgg cctgggcgcc gtctccggcg gcatcgcacc ggatctgcgc tacctgccgg
    17581 aaggccagga aggcgacgag atcttcctga accgcatccg ccatggcgcc acccgcgacg
    17641 gtcgcgttta tatgcccccg tttgaaggta tcctgtccca ggaagccatg tggaccatcc
    17701 gcgcctggct ggagaccgtt cgtgccgact gatcgtcgtc aattcctcac cgcggccggg
    17761 gcctgcctgc tggccgccgg cctgccggcc cgtgcagacg ggctggagga catccgccgc
    17821 cgcggttccc tgaccgtggc ggtgtataac gatttcgcgc ccttctccag caagggcaag
    17881 ggcatcgacg tggagctcgg cgaagccctc ggacgcaagc tcggtcttgc cacgcaggtc
    17941 atcggttaca acgccgatga ggacatggac gacgacctgc gcaacatggt ctggaaaggc
    18001 cattacctgg gcgcccgccc ctccgacgtg atgatgcacg tgccggtgga ttcctacctc
    18061 cagggcaaga acgacaaggt ccgcatcttc gggccctatc acctggagac catcgccgtg
    18121 gcgcgggatc cggcgcgggt cggcgccttc cgcggctccg ccgccaatgc gcttgaactc
    18181 ttcacccgcg agaagatcgc cgtcgaaggc cgcagcctgt ccgatgcctt cctgctgggc
    18241 gtgctcaatg gccgcctgcg ggacaacgtc gtgcattgca aggacatcgc cgaagccatc
    18301 gagaagctca aggccggcga agtcatcgct gtcatggggc cacgcagcga gatcgaagtg
    18361 gccctcggaa agggcacccg tttcatcgtg gacatcgtcc agatgccgga actcaagatc
    18421 aacaactggc ccctgggcat ggccgtcaag gccgatgaag cccccctcgc cgacgccctg
    18481 gccggcgccc tcaaggcact cgaacaggac ggtacggtgg cggagatctt tgcccgtcac
    18541 ggggtgacct acctcaaacc caccgtctga cacacccatg cgcctcacgc tttccggccg
    18601 gctcctgctg gcctgcacgg cctttcccgg gctcgtgctg gccgcgcctt ttgcctacgt
    18661 ccccaacgaa aagtcggcca ccgtctcggt gatcgacacg gccaccgaca gcgtggtgga
    18721 taccctgccc ggcggcaaac gcccacgcgg catcgccaca gatgggcagc ggctctacat
    18781 cagcgaggcg ccttcatcga gcctggtggt ggtcgatctg gcacggcgtc aacttctgcg
    18841 cagcatccgc ctgggtgaat cgcccgaagg ggtcagcgtg tcgacggacg gtcgtctgat
    18901 cgccgcggcg gtcgaggaat ccaacagcgt ctccctgatc caggccgcca ccggcaaggt
    18961 cctggccgac atcaaggtcc agggcaagaa tccggaacac gcggtgttca gtccggacgg
    19021 ccagtggctc ctggtcagcg ccgaagaagc ggagcaggta gacctgatcg acgtggccgc
    19081 gcgcaagcag gtggcgagca tcgccgtcgg ccgccgcccg cggggcatcg gctttctgcc
    19141 cgacagccgc cgcgcctatg tggcgtgcga actggacagc accgtatatg cgctcgacgt
    19201 gacgacgcgt caggcaaccg ccaccatcaa gacgggcacc ttctccaacg gaatcgccgt
    19261 ggcgcccgac ggcagccggg tgtatgtgtc caacggcaag gacggcacgg tcagcgcgat
    19321 ccgtacggcc gacaatcagg ttgaagccac gattccggtc ggcaaacggc cctggaacat
    19381 ggccctcacg cccgacgggc gcaagctcta cgtggccaac ggcaagtcca acagcgtatc
    19441 ggtcattgat acgacccgcc tcgaaaacat cggagacgtc cccgtcggtg aactgccctg
    19501 gggtgtcgtc attcgctgaa acgaggctgg aagctcaggc gaaagcgccc cgacttcccg
    19561 ccggtcgatt gcatgacgta gcgtcaactt gatacacctg ctccagattg gagcaggtgt
    19621 atcgcccggc cgctacacac catccaggaa cgcacgccca gagtgccgcc ggacgttctg
    19681 gatcacagct tcaggatcca ctcgatggcc gccttcaggt cggcatcgct gatcttgtcc
    19741 gggccgtgcg gcggcatcgg aatctgcccc cagttgccgg aacccccatt gcgcaccttg
    19801 gcgatcaacg ctgcctccgc atccttctga cccttgtact tggctgccac gtccttgtag
    19861 gccgggccga cccgcttttc ctcaaccgag tgacaggcca cacagcgggc cttcttgatg
    19921 atcgcttccg atgcgagcgc gggtgagacg accgcacacc ccagagcgat ggcacagacg
    19981 gtctggacaa atttcatgaa acgcctcctt cttcgagtgg tttgactggg caagtccggt
    20041 ctttccggca ggaaaaaatg gggcggccca tggccgcccg aaaaaccgcc ttgcctcctg
    20101 atttcagcaa aaaccatgca tgccacccgc cgttcaatca aaaatcaaat ttcccttttg
    20161 tttcataagc ttgacgaatc gcctcgaaag cccctcgcgt ccggttgctg aattcctgtt
    20221 cagccgctgc acacctgctc catcccgcaa cgccaagaaa ctcaccaccg gaacactgtg
    20281 cacccgcccc agtacccgcc tcggccgcaa agccgccgca gcgctccata aaattgaatg
    20341 aaatcagtca ctccagcaca cctgcccgat gggtttgggg gggacatgcg gccctgcatg
    20401 acaggcatgc ttcctgctgt catgggagcg tgagcccgat cgaccagcac cttatcaatc
    20461 tccccgtgga taccatccgg cgctcctggg aacgctgtgc gacccggggc ctgcgcgcgg
    20521 atgaacgtct accggacgac cccaacgcgc gcgtgcgcct gtcggaccgg ctggaggcca
    20581 acgcccgtct ggtggcgtac gcccagccca tcatggagca cctccaccag cagatcgcgc
    20641 gctcctcatc caccatcctg ctggccgacg attccggcgt gatcctgcgc tcggtgggcg
    20701 attcggactt tctcgaacgg gccgcccggg tggccctcaa gcctggccac tgctggtccg
    20761 agcacgtcat gggcaccaac gccatcggca cggcactcac ggaacaacgc ccggtcgccg
    20821 tgatcggcga ggaacacttt ctggaccgca acaagttcct cacctgcatc gccgcaccga
    20881 tccacgcccc caccggcggc gtgctgggca tcctggacat ctcgtgcagc gcccggctca
    20941 cccaggtgca cgccctggcc ctgctccaga ccaccaccga gatcatcgag aaccgcctca
    21001 tcgagaccct ggacgacgcg tcggtggtgc tgcgcttcca tcatcagccc gaagcgctcg
    21061 cgtcgccgct ggagggactg gccgtcttca acgaaagtgg cggcatgctg gcctgcaacc
    21121 ggcgcgctga acgcctgctg ggcttgcccg acacacgcca cagccagtcc gccttcaacg
    21181 acatcttcga gacccgctgg aacgtgctgc tcgaccacgc cctgcgcagc agcttccggc
    21241 ccaccaagct gcgctgcaac cgtggccgcg agtttgccgc cacggtgctc gtgagcaacc
    21301 tgcggcgcgt ccgcccggcc ggcgccgtac gcgatccgcg cgtcctgccg cgcccgcgac
    21361 cgcgcctgac gctggaagac atggacctgg gtgacaaggc cgtgggtacg gccatccgcc
    21421 gcgcccggcg cattctgggc ctggacatcc cgctgctgat ccagggcgag accggcaccg
    21481 gcaaggaagt ctttgcccag gccttccaca acagcggccc gcgccgtgac ggccccttcg
    21541 tggccatcaa ctgcgccgcc atcccgggca acctgatcga ggcagagctc ttcggttaca
    21601 ccgccggcgc ctacactggc gcccgtggcg aaggcgcccg cggcaaactg cgggaagccc
    21661 acggcggcac cctcttcctc gacgaaatcg gcgacatgcc cctcaaactc caggctgtgc
    21721 tgctacgggt tctggaaacc cgtcgcgtca ccccgctggg cggcggcccg gaagaggaga
    21781 tcgacctgtc gctggtgtgt gccagccacc gctccctcaa ggccttgtgc gaagtaggcg
    21841 agttccgttc agacctgtac ttccgcctgt cggccatgag cctggcgctc ccgccgctgc
    21901 gggagcgctc ggatctggaa ggcatggcac gacgcatcct gcaggaggaa tcgccggacc
    21961 gcccgatccg cctcgccacc tccaccctcg acctgatccg ccgccatccg tggccgggga
    22021 acatccgtca gctcaagaac gccctccgcc tcggggttgc catgctgggg gacgacgagg
    22081 aaaccctggg tcccgaccac ctgccccagg acctccttga cggcgacgac accgtccacc
    22141 ccgtgccggc cgccaaaagc ctgcgcgctg ctgaagtccg cctggtggaa gatacggtcc
    22201 agcgccacaa cggcaacatc tcggccgccg cccgtgagct gggcatcacc cgcaccaccc
    22261 tctaccgcaa gctgcgccag acgagcgacg actgaacccg accccgggcg tacgtcgtgc
    22321 gatactgggc gcctccgtgt gccgtccagc ctgcccgtga agatccgtcc gacctcccgt
    22381 ccgcgccgcc ccaccctggc ggccctgtgt ctggcgcccc tgtgcgccct cgccctgaat
    22441 gcctgctccg gcagaagcga tctgcccgaa cgcctgcgac aggagttccg tcccggcctg
    22501 tccatcgcgg aggcccgcga aaagctcacc gcccatggca cgacctattc gatccgcaac
    22561 accatggaat gcgaaggcct ggtgcgcaac tcaccgattg ccgccctgca gccgccccag
    22621 gggggactgt gcatcttcgg caagatcccg gtttccaagt cctggtttgg cgttcgctcc
    22681 gacgtgatcc tgcagctcgt gttcagcggc gatggggttc tgcaggacgg caactttgaa
    22741 gaagtcacca gcgcccagta ggcggcaggc cgtgcgcgcc agcctggatg agggagatca
    22801 ccgtcgccca gcaaaaagcc ccggcagggg ggctgaccgg ggctttcatt ccttgttcgg
    22861 cagggcttca ccctgccgct cctgcgctta cttcggcgac agggtcagga tccacttcac
    22921 cagcttcttg gcgtcggctt ccttgatggc caccttgttg gggggcatcg ccagaccgct
    22981 cacctggtcc ttccagtggc tgccgtaggg gttggagcct tccagcacca cacgggtcag
    23041 gaacgcgtcc gcacccggct tgcccttgta atgcttggcc acgtcctgcc aggccgggcc
    23101 gatgggcgcc atgccgttcg gtccgggcgt gcccgactcg atggcgtggc aggtcaggca
    23161 gccgctcttg gtggcgaggg ccttcatttc atcatccgct gcggccgacg cggtcgtggc
    23221 aaccgcggcc agaccggcca gcagcgcggc tttgcataag gcgttcatgg tgtttctcct
    23281 tggtaaaaaa gggtttgctc gctgaaacag ggactcacac ggcaggcaca ggcaccctgt
    23341 cgccaccgtg gagcggaaga ctcaatcgca ccgacaggcc gccgtccggc ccatctgaca
    23401 gccacacccg gccgccggcc atctcggccc gctcgcgcat gcccagcagg ccgccgcggc
    23461 cccgggcagc gacatccgta gcccgaccgc cgccattgtc actgaccacc aggtcgatcg
    23521 cgtccgcatc ggcccgcaac gacaaggtca cacgatctgc gagactgtgg cgcagcgcgt
    23581 tggtcagtgc ttcctgcagg gtccgataga gcgccagccc ggcggacggc ggcaaggcgg
    23641 gcagacgcgc cggcaggtcc gcctcaatag cgacctccgg cgcccggtgc tgccaggtgc
    23701 gcaccaggcc gtccagagcc gcggccacct gcgccccatc gtccaggccg cgggggcgca
    23761 ggcgcgccag cacagtgccc accagcttca ccagtcgtcc ggattcggtg cggatgtcgc
    23821 gggcgcaggc gctcaggcgc acggcgtccg cccggccggc gtggcgttcg atgaaggccg
    23881 cgcgcacgcc catcgcagtc agcgcctgac cgacttcgtc gtgcagttcc cgcgccagct
    23941 cgcggcgttc ggcttcctga agttccacca gacggcgagc cagcgtctgc cggtcggcgc
    24001 gctcgcgggc cagatcgccg gcaaggcgct cgatggccac cccgatacgc gagaactcgc
    24061 gtagttcgaa ctccggcagc cgcaccggcc ccgtgtcgcg ggcaatgcgg gcgagtcccg
    24121 cctccagctc gcgtaccggt ttgagggccc gatgggcggc cagccacgca acaaccgcca
    24181 tcgccacgga aaagaccagc agggtgagca tcagcccggc gccgtcccac aggatctcgc
    24241 gaatctccgc gtgcgcatcg gcgcgcacca ccagcacgcc atccgccagg ggaatgcgct
    24301 gttcaggcac ggtccgttcg cccagcaggg cggccgccag caggtccatc ggccccgcag
    24361 gcgcgtccga cacggagtgt tccagattgg agcgctctat ctgcagacgg acgtggcgca
    24421 ggggctccgc gcccagcagg cgctccagct cgtccacgcg catgccgggc gcagcacttg
    24481 ccgcgcggat cgcatccacc aggcgggccg acgcggccat ttcctgcgtc gcgtcccgct
    24541 gcagggcccc gccgatccac gcggcggctg ccgtcagcaa ggccagggca aagcccagca
    24601 cggtgccgaa cagcttccag cgcagatcca tcgacggaac ccccgaaatt ttcatgatca
    24661 attcaattgc aaggcatgtg ccatcttgcc ctgcgtcaag aaaaacggca cggaagccgc
    24721 agcgccgtgc cgttttccgc ctgcaacggc ccgccgcggc gggccgtcca tccggcgcgt
    24781 cttacttgct cttgtgcagc ttgaagaccc acacggaacc accctgttcg aggaagttca
    24841 cacgacgggc cacgtcgccg ccccacagag gcaccgcacc gccccagccg gacaccacgg
    24901 caacgaactg ctcgccacct tcggtccagg tgacgggggg cgccaccacg ccggagccgg
    24961 tctggaagga ccagacttcc ttgccggtct tggcgtccag cgccttcagg tagccttcgg
    25021 gggtaccgta gaaggtcagg ttgccgccgg tggtcaggac gccgccccac aggggagcga
    25081 agttcttgtt ttcccagacg atcttgcctg cagacggatc cacggcgcgc agcgcgccga
    25141 tgtagtcgtc gtgcagggcc ttgatggtga agcccgcgcc caggtaggcc gcgccgcgct
    25201 tgtaggagac cggctcgttc cagatgtcca tgccccactc gttggccggc acgtagaaca
    25261 ggccggtctt cgggttgtag gccatctgca tctggttctt gccgcccagg aaggacgggg
    25321 ccgcgaatac ggtggagccc ttcttgccat cggtgccggc agacgggtcg cccggacggt
    25381 tttcgtcgtt gtagatcgga cggccggtgg ccaggtcgat gcccttggcc caggtgatgt
    25441 ccttgacgaa cgggaaggcg ttcagcagct tgccgttggt gcggtcgatg acgaagaaga
    25501 agccgttacg gtcagccttg ccgccggcct tgatgacctt gccggacttc ggatccttgt
    25561 agtcgaagga cacgaattcg ttcacaccgt cgaagtccca gccgtcgtgc ggggtggtct
    25621 ggtagtgcca cacgatcttg ccggtatccg gatcgatggc cacggtggcg gaggagtaca
    25681 ggttgtcgcc ggggcgcagg tggctgttcc acggcgccgg gttgccggtg cccgcgaaga
    25741 ccaggttggt ctccggatcg taggtggcgc cgttccaggt ggccgcgccg ccggtcttcc
    25801 acatgtcgcc ggtccaggtg gcgttggtgg tgccggagat gccgttctcg accttctcgc
    25861 cattcaccca cttgtaaccc atgtgacctt cgacggtggg acgcatccac accatcttgc
    25921 cggtcttggc atcgcgcgct tccatgcggc cctgcacgcc gaattcgccg ccggacacgc
    25981 cggtgatgac caggcccttg acgatcaggg gcgccgcgga ggcggagtag ccggccgcgt
    26041 agtcgtcgat cttgtccttc cacacgacct tgccggtgtc cttgttcagg gccacgagct
    26101 gggcgtcgag ggtggcgaag atcaccaggt tgtcgtagag cgcagcgccg cggttgatca
    26161 cgtcacagca gggcatgatg ccttcgggca gacggtgctc gtacttccac agcttcttgc
    26221 cggtcttggc gtccagggca aacagacggc tgtaggaagc ggtcacgtac atcttgccgt
    26281 cgaacacgac cggctgggat tcctggccgc gctgcttttc accgccaaag gagaaggacc
    26341 acaccggaac cagatccttc acggtcttgg tgttgatctg gtccagggcg ctgtagcgct
    26401 ggccttgctg gccgagaccg aaatggacca cgtcgccggg ggtcttggcg tcgttgagaa
    26461 tgtcggcgtc cgtcacttcc ttggcgtggg cagcgccggc aaccatcacg gcggcggcga
    26521 tcaccgtcat tgcaaacggc tttttcatct tttgactcct ttggatcatt gagtgtctcc
    26581 tttgagaggg tgctgaagca ctattcgggg cggccacctc ccggcccaac caaaccgtgc
    26641 agcaatggcg gtctgcaaga gctgtgccat tggtcgtgac gaaattccac acgccccgca
    26701 tccgggaaaa tttcccgata caaaaccgcg gcttggacat gccccccgtt ccccggaatg
    26761 cgcgtcgcca cgcgcattcc ggcacacact gcgccatcgc tgcttcattc caaaccaagt
    26821 gctccaaagt gcagcacacc gccttaacac acccgcactg caacatccgg agacccccgc
    26881 cacggaaagc cccggatcgc tgaacacacc ccccgaccag ccccttcggc cggcacggac
    26941 actgtagaga tcccatacga tttcatggca caggtcttgc agaatccggg gatcgacaga
    27001 gcgtgccgca ccgacgagcc gccacgagga gacaccgaga ccatgccgcg catcatcacc
    27061 gccctgatgt tcgccctggt ctgccacgcg gccctggccg ccgcaccggc agacccgccc
    27121 gaccccctca actccggccg ctgggccgac atgcgcaagg aagtgctgcc ggccgatgcc
    27181 cgcaccgtct tcgacgagcg catccgcgtg atcgccccga tcagcgccga gaaccccctc
    27241 aacgtcccca tcaccgtgga cgccagcgcg ctggacggtg tcgaggaagt gcgcgtcttt
    27301 gccgactaca accccatcgt gcagatcgtc cgcttcgagc ccggcggggc gctggcgacc
    27361 ctcggcttcc gcgtcaagct ccagcaatcc acccccatcc gcgccgccgc ccgtggcgcc
    27421 gacggtctct ggcacgtggg cggcagctgg gtgaacacca ccggcggcgg ctgcaccctg
    27481 ccctccaccg gcagcagctc gcccgaatgg cagcgccgcc tcggcgaagt gagcagccgc
    27541 gtgtgggcgc gcaaggaagg cggcgagcgc atccgcctgc gcatcgtcca cccggaggac
    27601 accggcctcg ccgccggcat cccggccttc tacatcgaag acctgcgcct caccgacgac
    27661 ggcgggcggc cgctgatgcg tgtgcgcggc ttcgagccca tcgccgagaa ccccgtgttc
    27721 accgtggaca tccccgccgc gcacaccggc cacggctacc ggatcaacgg ccgggacaac
    27781 aacggcaata ccatcgccgc ggagatcgac aaatgagcgg cgcggccccc atgcggcttc
    27841 gcgtgccgcg cctgctgcgc gccccggcgg cggccctcgc cctgggctgg accctcctcc
    27901 tcggcgccgc cgacttcgac tacgccctgc agccccggcg catcgccgac gacacctggg
    27961 tgctcaccgg ccgcaacgag gacttcaccc tcgccaacgg cggcaacatc gtcaacaccg
    28021 ccttcatcgt caccgccgac ggcgtcgtgg tcatcgacag cggcccctcg cgacgctatg
    28081 gcgaacagct ccgccaggcc atcgcgcagg tcaccgaccg gcccatccgc ctcgtcctca
    28141 acacccacca tcacccggac cacatcctcg gcaaccaggc cttcccggcc gacaccctgg
    28201 ccgccctcgc cggcaccgcc gacggcatcc acaccgaagg cggcgccttc aacgacaaca
    28261 tgtaccgcct ggtgggcgac tggatggccg gcaccgaagt cgtcaccccc gcccgccgcc
    28321 tgccctccgg catccaggag atcggcggcc accgcctcga attcatcgcc ctggacggcc
    28381 acaccggcgc cgacctggct gtcctcgacc gcagcaccgg cgtcctcttc gccggcgacc
    28441 tggtcttcca cgaccgcagt ccgaccaccc cccacgcccg cctgcccgcc tggaacgccg
    28501 ccctcgaccg cctccaggcc agcggcttcc gcgtcctggt gcccggtcac ggccctgtcg
    28561 ccggcgacgc cggcccgatt cgccagaccc gcgcctggct cgcctggctg gatggcaccc
    28621 tgcgcagcgc cgccgaagac gggctcgaca tgaacgaagt catggccctg ccgattcccg
    28681 ccgaattccg ccgcctcgcc gtggtcgaaa ccgagtacag gcgctccgtc ggccagctct
    28741 atcccgccta tgagcaggcc gccctgcacc gcccccggag gtccggccca tgatgacccc
    28801 tgccctgccc cacgactggc cctggcagcc cgcgccggcc ctgccgcgca ccctgcgcct
    28861 cgccctgacc gcctccatcg gcgtccacgt ccttgccctg atgggccact acggcctgct
    28921 gccgaatccg tccgatagcc ccgcccccct tcaggtcgaa ctgcgcctgc cgccccccgt
    28981 cgcgccagag cccgtccgcg ccgtcgcccc gccgctgc
//
LOCUS       DAOQMM010000039        28985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_460_length_28985_cov_45.890321, whole genome shotgun sequence.
ACCESSION   DAOQMM010000039 DAOQMM010000000
VERSION     DAOQMM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 28985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28985
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_460_length_28985_cov_45.890321"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLH85_08475"
     CDS             complement(<1..425)
                     /locus_tag="PLH85_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNB64637.1"
                     /translation="MKILVLPGDGIGPEIMEQALRVLDALRGDGLSFEVEHGLLGGSA
                     VDATGTPFPEATRALARAADAVLLGAVGGPQWDALPRDQRPERGLLGIRKELNLFANL
                     RPAILYPELANASTLKPEVVAGLDILIVRELTGDIYFGQP"
     gene            complement(472..1110)
                     /gene="leuD"
                     /locus_tag="PLH85_08480"
     CDS             complement(472..1110)
                     /gene="leuD"
                     /locus_tag="PLH85_08480"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNB64638.1"
                     /translation="MKPFTRLDGLVAPMDRANVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDIGQPGMDNTGRPKNPEFVLNQPRFQGAQILLTRDNFGCGSSREHAPWALEDF
                     GFRVLIGTSFADIFFNNCFKNGLLPIRLPAAEVDALFGQCLATPGYHLTVDLAQQTVT
                     RPDGKAIPFEVDPFRRECLLNGWDDIGLTLRHADKIREFEARRRIEQPWLFA"
     gene            complement(1110..1235)
                     /locus_tag="PLH85_08485"
     CDS             complement(1110..1235)
                     /locus_tag="PLH85_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HNB64639.1"
                     /translation="MRKFVALMGVFAVLLLTGCNTVHGIGKDIEKGGEAIQKVAK"
     gene            complement(1255..2664)
                     /gene="leuC"
                     /locus_tag="PLH85_08490"
     CDS             complement(1255..2664)
                     /gene="leuC"
                     /locus_tag="PLH85_08490"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNB64640.1"
                     /translation="MHAQTLYEKLWSSHVVHVEDDGTALIYIDRHLVHEVTSPQAFEG
                     LKLAGRKPWRVNSIVATADHNTPTDHWDQGIQDPVSRQQVETLDANIREVGALAYFPF
                     MHERQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVMATQCL
                     LQKKSKTLLVRVDGALGPGVTAKDVVLAIIGRIGTAGGTGYAMEFGGSAIRSLSMEGR
                     MTVCNMAIEAGARAGLVAVDDKTIAYLKDRPFSPRGDQWDRAVTYWRTLHSDEGAQFD
                     AVVVLNGAEIKPQVTWGTSPEMVVPVDGSVPDPALEKDPVKREGMERALQYMGLTPRT
                     PINKVAVDKVFIGSCTNSRIEDLRAAAAVARGRHKADSVRQVLVVPGSGLVKHQAEAE
                     GLDKIFIAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSP
                     AMAAAAAIAGHFVDVRNLA"
     gene            complement(2694..3542)
                     /locus_tag="PLH85_08495"
     CDS             complement(2694..3542)
                     /locus_tag="PLH85_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB64641.1"
                     /translation="MMSVWKGMLPLGAAVLMVTACQTVQTTQSGAVGVERRQSMLVSS
                     REMEQASAQSYHKILQQAAEKNALNRDRVQVERVRVVARRLIPATGAFRPDAPGWRWE
                     VNVLTSEELNAWCMAGGKIAFYSGIIERLQLSDDEMAAIMGHEIAHALREHARERASQ
                     AMVTNIGVSIVGAVLGAGQAGTNLIGQVANVSFGLPNSREQETEADRIGVELAARAGY
                     DPRAAIVLWQKMAKVSNGAPPQWLSTHPSNETRQADLQQYAARVMPFYEEARNARPTQ
                     GSKPVK"
     gene            complement(3567..4712)
                     /locus_tag="PLH85_08500"
     CDS             complement(3567..4712)
                     /locus_tag="PLH85_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB64642.1"
                     /translation="MIPYWRLSGYYFFYFAFVGAFSPYFSLYLQSIGRSAAQIALLMS
                     LLSVMRLVAPNLWGWLADRLGARVPIVRLSAVLSLAGFAGFLMTESPVGLFASMAVLA
                     FFWSAALPLVEALTFSHLGAHASRYGSIRVWGSVGFILAVLGLGYVLDGAPIRTLLWI
                     TLGLLGGILVYAAVLPEGAAPAHSMNPASLGEVLRRREVRALLTACFFMSAAHGALYV
                     FYSVHLVQLGHHKTLVGWMWTLGVVAEIAVFMAMPQILRRYSLRGILLFSFAVAVVRF
                     LLIGWGANSLAVLILAQIGHGATFGAYHAAGVAAINRWFGPQLQARGQALYGSVSFGA
                     GGMVGGVVSGYTWDSIGAGWTYTLGSAFALMGLAVLLAGWRPAVREA"
     gene            complement(4819..5061)
                     /locus_tag="PLH85_08505"
     CDS             complement(4819..5061)
                     /locus_tag="PLH85_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNB64643.1"
                     /translation="MEQHDLNHEFPEYRDQIHSLKVSNNHFSRLFDEYHDVNRHVVRV
                     ELNAELATDFELEELKKRRLKLKDELYDMLKGAVVN"
     gene            complement(5151..6293)
                     /gene="aroC"
                     /locus_tag="PLH85_08510"
     CDS             complement(5151..6293)
                     /gene="aroC"
                     /locus_tag="PLH85_08510"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNB64644.1"
                     /translation="MSGNTLGTLFTVTSFGESHGPAIGCVVDGCPPGLALCEADIQAE
                     LDRRKPGTSRHVTQRREPDTVEILSGVFEGQTTGTPIALLIRNQDQRSKDYGNIAETF
                     RPGHADYAYWEKYGIRDHRGGGRSSARETAVRVAAGAVARKWLAERYGIVIRGYMAQL
                     GPVEIPFAAWDDVDDNAFFAPNAALVPALEDYMDELRKSGDSVGARINVVATGVPVGW
                     GEPVYDRLDADIAHAMMGINAVKGVEIGAGFASVAQLGTQHSDEMTPEGFLSNQAGGV
                     LGGISTGQDILVSIAIKPTSSTRLDRRSINKAGEPVVVNTHGRHDPCVGIRATPIAES
                     MLAIVLMDHALRHRAQNADVACPTPPVAGLAPDGVQGVPSPKGSKG"
     gene            complement(6398..6733)
                     /gene="ilvN"
                     /locus_tag="PLH85_08515"
     CDS             complement(6398..6733)
                     /gene="ilvN"
                     /locus_tag="PLH85_08515"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNB64645.1"
                     /translation="MSDQTSQLSEYIPDVPEHTGFHKVVLELAVANHPGVMSHICNLF
                     ARRAFNVEGILCMPVGEGGTSRIWLLIFEDERLEKMIRQVEKLEDVLYVRQHAGDHPV
                     FGRLDEFFQ"
     gene            complement(6752..8416)
                     /gene="ilvB"
                     /locus_tag="PLH85_08520"
     CDS             complement(6752..8416)
                     /gene="ilvB"
                     /locus_tag="PLH85_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNB64646.1"
                     /translation="MMQMTGADLIMKLLERQGIRTVAGVPGGAALPMYDALTRSESIH
                     HVLCRHEQGAGFIAQGMARVSGRPEVCLASSGPGATNLITAIADAKLDSIPIIALTGQ
                     VPLSMIGTDAFQEVDTYGLTIPITKHNFLVRSAKELLTVIPAAFRIAMSDRPGPVLVD
                     VPKDVQNQMISFEDFPDVVVPDAPPPVDIAAVEEAARMIEAAERPVLYLGGGVIHSGA
                     SQLAVTLAEQASLPTTMTLMALGAMPMDHPLAIGMLGMHGARYTNYVLEESDLIICVG
                     ARFDDRAIGKAAEFCPNAKIIHIDIDPSELHKIKTAHVAIAGDVGQVLGELLPRVKPQ
                     LRKRWLSHVESLKGRFPLAMPGVDDPRTPYGLIHAVASCLDDNAIITSDVGQHQMWVA
                     QAYPFRRARQWLNSGGLGTMGFGFPAAIGAALAEPERTVVCFSGDGSLKMNIQEMATA
                     AQENVNVKIVLFNNQSLGLVHQQQNLFYGKRIMASRYEHPTDFLKIAEGFGMRAVNLD
                     PSPNPRATLAEALNTPGPCLIHATIDPNEFVYPMVPPGAANSEMIG"
     gene            complement(8733..10406)
                     /locus_tag="PLH85_08525"
     CDS             complement(8733..10406)
                     /locus_tag="PLH85_08525"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNB64647.1"
                     /translation="MDKIWLQSYPPGVPPEVDLDEFSSLVDLFEQSVRKFAARTAYVN
                     MGKSLTYGELDELSARFAGWLQGELGLGPGARVALMMPNCLQYPVAMFGVLRAGLTVV
                     NVNPLYTARELEHQLKDAGCEAIVIVENFAHTLQDVLPSTPVRHVVTTGLGDLLGFPK
                     SLLVNFVVRHVKKMVPAWSLPGSIAFREALAKGAAHPLRPVMLGHEDLAFLQYTGGTT
                     GVAKGAMLTHGNIVANLQQAHAWIGPYVREGEELIVTALPLYHIFSLTANCLTFFKIG
                     AENLLITNPRDIPGFIAELAKVRFTAITGVNTLFNALLNHPDFEKLDFSSLRLALGGG
                     MAVQQAVAERWKAVTGRPLAEAYGLTETSPAVCINPLDLPEFNHSIGLPISSTDVSLR
                     DDEGYEVLLGEPGELCVKGPQVMKGYYNRPEDTARVFTRDGYLRTGDVAVIAPDGYVR
                     IVDRKKDMILVSGFNVYPNEVEDVVASHPGVLEVAALGVPDERSGEAVKIFVVRKDPT
                     LTAEALIAHCRQGLTGYKVPHYVEFREELPKSNVGKILRRALRDASTPK"
     gene            complement(10641..11090)
                     /locus_tag="PLH85_08530"
     CDS             complement(10641..11090)
                     /locus_tag="PLH85_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB64648.1"
                     /translation="MLVSEILAIKGKVLFTIAANKTLADAVAVMTEQDVGSLVVFDKG
                     HMAGLLTFREVLVATQKAGAGWESLPVTEAMLKTPVVANPSMEMDELRRQMVEHHQRY
                     LPVMDDTMLMGVVSFHDVAKAVLEEQSFENRMLKNYIRNWPSEEGQV"
     gene            complement(11186..14491)
                     /locus_tag="PLH85_08535"
     CDS             complement(11186..14491)
                     /locus_tag="PLH85_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB64649.1"
                     /translation="MSVPHDSPVERARRALDPARSVVVEACAGSGKTWLLVSRLLRLL
                     LAGARPGEILAITYTRKAAREIEERLRQWLRLLAGAPDAAVLDFLSERGLEAAAAQAA
                     LPRARGLLEAVLGAEPPMTITTFHGWFARIVGGAPFGSGLAGFRLSEAEAPLLDEAWA
                     RFARSCAAEPAGVEAAALLRLFDTVGAARTRALLQAFVNRRAEWHAYLDDGRDGLDVA
                     LARLQADLGVVAPGAALPAFLATAGLAEAVEALARLLDANTDSDRARAAALRAGFAAD
                     DAATRFAALKAAVLTQKGEPRAVKASAAQAKRLGEAGQTRFLDLHGRLTDQILAAADA
                     VLEERIFVLNRDVLSAAGGLLHALEAHKAERRLMDFADLEWHTDRLLSDPEQAPFLQA
                     RLDARYRHILLDEFQDTNPLQWRILVAWLDAYGLDGTPPRIFMVGDPKQSIYRFRRAD
                     FRIFTHAAEWLARRFDADRLANDATFRNAPAVVEVVNSLFAHEPAFVGFRPQTAVRAG
                     LPGQVRLLPLIEDDEAAGGTASNVATDALRDPLRVAPVLAENLRRRREALAVAEHISA
                     LVGRWQVAGEHGPRPARYGDVLILTRRRSVLPEFERALRDAGIPYLSVSRGGLLKTLE
                     AADLVALLRWLASPADDLALVHALRSPVFGVSDDALLRLAARPEAGWWARLGAMLAEG
                     AADAALERAGHRLADWKTQAATLPVHDLLDRIFHEGQVLARYHAVVPPPMAAGVRANL
                     EAFIALALELDGGRYPSLPRFIDELGRLSGAGDDEAPDEGLIPGDGDDADGRVRIMTI
                     HGAKGLEAPVVWLIDARNGHQKPDTDQVLLDWPPGDIRPRHFSLATRKDERGRCRAAL
                     FEAEEQARAREDLNLLYVAITRASQYFLASGSLPTRDAAAPNPYQRIAAALGRLGAEA
                     GTWGSPPVEVAAAPAPAPAVAAEVAPTIAPVPVGQYRSVADAVAESAGTLFGTAFHTA
                     LECLTEARSTAGLDPAAVELAGRLVARPQLARFFDPAACRWARNEVEFVDAAGRLGRI
                     DRLVDDGETLWVVDYKTGAPDERLLDVYRAQVRTYGRAVAALWPGRPLRAVLVFAGGE
                     ALEVAVDEAGNAA"
     gene            complement(14488..17145)
                     /locus_tag="PLH85_08540"
     CDS             complement(14488..17145)
                     /locus_tag="PLH85_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNB64650.1"
                     /translation="MTVVTLPSGGDFLKACAGHVLAAAPDPAELSRAGLILPNLLLAS
                     DLRAAFHAAAGGAVLLPRLQTLPAVLEPWLAAQPHLPDSRRLFALYHALKARRWLDEA
                     SLWDVCAELVALFDELTLAGVGLPATEAELQMRLAHAYARAVDEPLRFEARLVHVLWQ
                     ADNRGVPSRAAARLLAARAWAAARTAPLFAIAEGALSPFERDVYAACAERVPVTVFVP
                     DRWRATDGLARVLNAAWPAGADAPPLALRAAGLADTGLPLGGRVRLFAAHSLEQEARA
                     VAATVRGWLASGKRRIALVAADRVAARRTRALLERDGVLVEDETGWKLSTTRAAALID
                     AWLSVLSSDGYHRDVLDLLKSPFVAGSTGEADRAAGIEAVARLYAHHGLASGLDAVAL
                     LAAGDAPAAALVRLLLEARSVFPRTAATPAQWLDRLGAALAALGAVTALASDAAGADI
                     LAWIAARRAELADEDARIGFHEWRQWLDRELESVMFRDRSVDSPVVMTHLAAARLRRF
                     EAAALIGADADNLAPQDRRPVFAHEGVRAELGLPGSAQARARLHDDLAGLLTLADAVC
                     ISWQHLRDGEAVLPCAAVDVLSLVHQQAFGESLRAPVPGSFEADVPVVGQPVPAPVLM
                     PEQVPTRISASGLASLIACPYRYFARHVLGLGEAEEVSEALEKADYGQMIHAILQAFH
                     ARFPQIATEDDAVLVAALEDESARVFGPALARNFLEHAWAARWRAQLPSYVAWQKARE
                     AEGWTFAAAEESAWRDFALADGARVTLHGRLDRIDRRRDGTEAVLDYKSRPLGALRKQ
                     AADVDDVQLAVYTLLRGTAVREAAYVALDDGAVGAAPLDDPVAMAEVQAERLVTAFDA
                     LHAGAPLPAHGVACTHCEMRGLCRKDYQA"
     gene            complement(17188..17511)
                     /locus_tag="PLH85_08545"
     CDS             complement(17188..17511)
                     /locus_tag="PLH85_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNB64651.1"
                     /translation="MTYVVTENCIKCKYTDCVDVCPVDCFREGPNFLVIDPEECIDCT
                     LCVAECPAEAIFAEDDVPAGQESYIALNAELALTWTPIVERKDPLPDADDWRGVPDKL
                     QYLER"
     gene            17836..18162
                     /gene="trxA"
                     /locus_tag="PLH85_08550"
     CDS             17836..18162
                     /gene="trxA"
                     /locus_tag="PLH85_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNB64652.1"
                     /translation="MSEHIHYVTDANFEAEVLQSQTPVLVDYWAEWCGPCKMIAPILD
                     DVAKEYDGKLKVAKLNIDENQDTPAKFGIRGIPTLMLFKGGNIEATKVGALSKSQLTA
                     FIDSHL"
     gene            18335..19594
                     /gene="rho"
                     /locus_tag="PLH85_08555"
     CDS             18335..19594
                     /gene="rho"
                     /locus_tag="PLH85_08555"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNB64653.1"
                     /translation="MHLSELKSLHVGQLLEMAIANEIEGANRLRKQELVFALLKNRAK
                     KGEPIFGDGVMEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDTVEGEIR
                     TPKDGERYFALVKVDKVNFEPPEASKHKILFENLTPLHPNKVLKLERDIRGEENITSR
                     VIDMISPIGKGQRGLLVAPPKSGKTVMLQHIAHSITTNHPDVVVIVLLIDERPEEVTE
                     MQRSVKGEVVASTFDEPAIRHVQVAEMVIEKAKRLVEHKKDVVILLDSLTRLARAYNT
                     VVPASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRVYPAINVNRSGTRREELLLKADVLQKVWVLRKLLYGMDD
                     IEAMEFLLDKIKATKSNGEFFDAMRRG"
     gene            19721..19805
                     /locus_tag="PLH85_08560"
     tRNA            19721..19805
                     /locus_tag="PLH85_08560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19755..19757,aa:Leu,seq:tag)
     gene            complement(19855..21066)
                     /locus_tag="PLH85_08565"
     CDS             complement(19855..21066)
                     /locus_tag="PLH85_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNB64654.1"
                     /translation="MKEFAEIADWLGTRGAVHAVPLVESLPAPHFRMDGAEHVSFSTN
                     NYLSLATSPRMIRAAMTGLQRYGVANCESRLLGGDLDVYRTLEDKLARLKGKDTALLF
                     ATGYLTNLGVLAALPRIGQVARTYGYRPRTRHTYAFFSDEYNHVSIREGIRLSGADRF
                     TYRHLDLAHLEHLLNQSAATSRVIVTDGVFSQDGDIAPLPELLALAERYDAMVYVDDA
                     HGSGVLGAHGGGITEHFGIDDPRLICMGTLSKAYGAIGGFIATEQAIGDLLRLTCTAY
                     GFTSTLPPDQAFAVSEALDMVQDEPERLQRLWANQRYFVERMRGLPYRLVATETPIVP
                     VLIGDEALTDHLAACMHEAGLHVDSVKFPAVPRRQGRLRVILNAGHTQEDIDRLVAVF
                     ERHQALCRAAA"
     gene            21264..22433
                     /locus_tag="PLH85_08570"
     CDS             21264..22433
                     /locus_tag="PLH85_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB64655.1"
                     /translation="MLTALRTLPSTVWLLGLVSFFNDTAGELVYPVLPLYLASVLMAG
                     PRALGLIEGIAEATGSLLKLVSGVLSDRLRHTKRWVVAGYAIAGLSRPLLTLATSWPL
                     VLALRFADRIGKGLRSSPRDALLAATVAPQHRGLAFGLHRACDNVGSVAGPLLAAALL
                     ARQVPLERIFLWASLGGAVAVALTLLIREPVRVESRPALRPGWTLATLPPAFRRYLAV
                     LVLFTLGNASNMFLLLKSRDAGVADYQVPLLWALVSFTAALFSTPLSALSDRIGRSRL
                     ILAGWSVYALFYLLLGLAGQAWMMWPLFAFYGLFMAATEGVEKALVADLVPQEQLGTA
                     FGWFNLAAGLTLLPASLIFGALWDALAPTAAFAFSAACAALAALLLRLWVHPERP"
     gene            22517..25915
                     /locus_tag="PLH85_08575"
     CDS             22517..25915
                     /locus_tag="PLH85_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64656.1"
                     /translation="MPDQHAPPVQRVVKIRRDYNTWVARETLEDYALRFTPRNFRRWS
                     ELRVANTAFGAASFLVLEAVGATMLVNAGFINAFWAILATGLIIFLIGLPISHYAARH
                     GLDMDLLTRGAGFGYIGSTLTSLIYASFTFIFFALEAAIMAYALELAFGVPPAWGYLL
                     CALVVIPLVTHGITAISRLQAWTQPLWLVLLILPYLFLLTQEPGVLAELVRYPGANGD
                     GGAFSPQLFGAALTIGIALVTQMGEQVDYLRFMPERTPANRRAWWLGMILGGPGWILP
                     GIAKMLGGALLAYLALRNAVPADKAVDPNQMYLIGFSHVFDHTGLAIAATALFVVVSQ
                     LKINVTNAYAGSLAWSNFFARLTHSHPGRVVWVVFNTVIALLLMELDVFQALGQVLGL
                     YSNIAISWMAAVVADLVINKPLGLAPPGVEFKRSHLYDINPVGVGAMGLASLLSVATF
                     AGAFGATLQPYASFVALGVAFVTAPLIAWATGGRYYLARPAVATGAGLQRCVICERDY
                     EGEDMAHCPAYQGSICSLCCSLDARCHDQCKPHASVAEQWKALLHHLLPHSARAYIDT
                     GLGHYLLLMAGVTALLALVLGVLYTHEALLLGPDQPDLVASLRASFLKLFAALLLLSG
                     VGAWWMVLTQQSRRVAQEESNRQTELLMQEIESHRRTDEQLQRAKEAAEQANQAKSRY
                     ITAISHELRTPLNSILGYAQILAADDAIPPGRRQAVSVIRKSGDHLLSLIEGTLDIAR
                     IEGGKVALEVRPLEFPAFVQQIVAMFELQARNKGLDFTYTPHGALPAVVRADERRLRQ
                     ILINVLGNAVKFTARGGVELAVSYRREMATFDIRDSGPGIPAADLERIFEPFTRGRTG
                     GSPGSGVGLTIARMLTTLMGGELQLSSTPGEGSQFRIRLFLPQLHTAQAEALPRAARV
                     GYVGVRRRILIVDNEKVDRDMLQSVLDPLGFQIEQVGSGEECLVAVPRFAPHLIFMDL
                     AMPGIDGWETIRRLRAGPAPLAGADVKIAILSANAFDKGLDNDAGVAPQDFIVKPLRV
                     DALLDWIGRSLDLEWVHADSPPPPAPAPAAPPPLIAPDASHLAALDELIALGYLRGIL
                     GKLAEIERLDPCHGEFVRVLREQARQFQFDAMKAILRKARDDRP"
     gene            25902..26834
                     /locus_tag="PLH85_08580"
     CDS             25902..26834
                     /locus_tag="PLH85_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNB64657.1"
                     /translation="MTALDRSISDIVLIVDDVPENLALLHDALDDAGHTVLVATDGES
                     ALLRARQSLPDVILLDAVMPGLDGFEVARRLKADFTTRHIPIVFMTGLTETEHVVAAF
                     AAGGADYVTKPIRPAEVLARIAAHVQNARQMKQARSALDAFGQATVAVRVADGRLVWQ
                     TPLARRLLRDFFAAAGEDAPLRLLDWIHDAELARREGREPVGLLAADGSRRLLASFHD
                     QTGDAEWLVVLREENDASAIESLIAAFRLTQKEAEVLYWVTQGKTSKDIGDILGSSPR
                     TVNKHLEHVFEKLGVETRTAAANLAMSRMRSGSA"
     gene            complement(26859..27302)
                     /locus_tag="PLH85_08585"
     CDS             complement(26859..27302)
                     /locus_tag="PLH85_08585"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNB64658.1"
                     /translation="MALVSDLPTVAHAIQQSVAPVFLLTGVGSLLGVLANRLGRIVDR
                     SRALRAAGAEAVARAADEMALLQRRSRLVHAAIRYCTLCALLICVVIAVLFVGVELGS
                     DFSSLVAVLFIGGMASLIAGLVCFLREISVATSRLEDRPPPPPPA"
     gene            27301..27447
                     /locus_tag="PLH85_08590"
     CDS             27301..27447
                     /locus_tag="PLH85_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64659.1"
                     /translation="MTQRPPNPRRKAPPPAHAARPAPWPATRHGPCTACLPLLLARVI
                     LALL"
     gene            complement(27605..28735)
                     /locus_tag="PLH85_08595"
     CDS             complement(27605..28735)
                     /locus_tag="PLH85_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNB64660.1"
                     /translation="MGAMGRDGARFLANFRSEQESAALYREMAAQESQAEVAALYRKL
                     ATVEETHAEFWRRRIEKAGGTVPQGLGWRARCLLWATRRFGPQAVLPLVAGDEVRNRG
                     VYDDQRETAGTGMRAQERSHARMLSLLASRSTTGWNGALYSRLEGRHGAGAANNLRAM
                     VLGANDGLVSTFCLVMGVAGTAAGPQALLATALAGTLAGACSMAMGEWISVQSARELQ
                     EKQIASEAEELAQVPAEEQEELSLIYQARGFSQDEARQMAARVIGDKDSALDTLVREE
                     LGINPEDLGGSALEAAASSFLVFLVGAMIPALPMFLAPRASILLASTVSSALGLFVLG
                     AAIAVFTGKHPLLAGARQLLIGGAAAGVTFGIGRLFGVAVGG"
BASE COUNT         4680 a        10554 c         9376 g         4375 t
ORIGIN      
        1 ggctggccga agtagatgtc gccggtcagc tcgcgcacga tgaggatgtc cagcccggcc
       61 acgacttccg gcttgagggt ggaggcgttg gcgagttccg ggtacaggat ggccggacgc
      121 aggttggcga agaggttgag ctccttgcgg atgcccagca ggccgcgttc ggggcgctgg
      181 tcgcggggca gcgcatccca ctggggaccg ccgacggcgc cgaggagcac cgcgtcggcc
      241 gcccgggcga gggcgcgggt ggcttccggg aaaggcgtgc cggtggcatc cacggcgctg
      301 ccgccgagca ggccgtgctc gacctcgaag gacagaccgt ccccgcgcag ggcgtcgagc
      361 acgcgcaggg cctgctccat gatttcggga ccgatgccgt cacccggcag aacaagaatc
      421 ttcatggcgt cgtcagaata aggcggtgaa aaagtccggc gcggcgccgg ctcaggcgaa
      481 cagccagggc tgctcgatgc ggcggcgagc ttcgaactcg cggatcttgt cggcgtggcg
      541 cagggtcagg ccgatgtcgt cccagccgtt gagcaggcat tcgcggcgga acggatccac
      601 ctcgaagggg atggccttgc catccggccg ggtcacggtc tgctgggcca gatccaccgt
      661 gaggtggtag ccgggcgtgg ccaggcactg gccgaacagg gcatccacct cagccgccgg
      721 caggcggatc ggcagcaggc cgttcttgaa gcagttgttg aagaagatgt cggcgaagct
      781 ggtgccgatc agcacgcgga agccgaaatc ctccagcgcc cagggcgcgt gctcgcggga
      841 actgccgcaa ccgaagttgt cgcgggtgag caggatctgg gcaccctgga aacggggctg
      901 attgagcacg aactccgggt tcttcgggcg gccggtgttg tccatgccgg gctggccgat
      961 gtccaggtag cgccattcgt caaacaggtt ggggccgaag ccgctgcgct tgatcgactt
     1021 gaggaactgc ttggggatga tcgcgtcggt atccacgttg gcgcgatcca tcggcgccac
     1081 gagaccgtca aggcgggtga agggcttcat tatttggcca ccttctggat ggcctcgcca
     1141 cccttttcga tgtcttttcc gatgccgtgc acggtattgc agccggtcag cagcagcacg
     1201 gcgaacacgc ccatcagtgc cacaaattta cgcatggtcg tctccttgga tgattcaggc
     1261 caggttgcgc acgtcgacga aatgtccggc aatggcagca gccgccgcca tggccggact
     1321 caccaggtgc gtccgaccgc cggcaccctg gcgaccttcg aaattgcggt ttgacgtgga
     1381 cgcacagcgc tcgcccggtt cgaggcggtc ggcgttcatg gccaggcaca tggaacaccc
     1441 cggctcacgc cattcgaagc ctgcggcgat gaagatcttg tccagtcctt ccgcctcggc
     1501 ctgatgcttg accagcccgg agcccggcac caccagcacc tgccggacgg aatcggcctt
     1561 gtgacgcccg cgagccacgg cggccgcggc gcgcaggtcc tcgatgcgcg aattggtgca
     1621 ggaaccgatg aacaccttgt ccaccgccac cttgttgatc ggcgtgcgcg gggtcagacc
     1681 catgtactgg agcgcccgct ccatgccctc gcgcttgacc ggatcctttt cgagggccgg
     1741 atcgggcacg ctgccatcca ccggcacgac catctcgggc gaggtgcccc aggtgacctg
     1801 cggcttgatc tcggcgccat tgagcaccac caccgcatcg aactgcgcgc cttcgtcgga
     1861 gtgcagcgtg cgccaatacg tgacggcgcg atcccactga tcgccgcggg gcgagaaggg
     1921 gcgatccttg agataggcga tggtcttgtc gtcgacggcc accagcccgg cgcgggcacc
     1981 ggcttcgatg gccatattgc agaccgtcat gcgcccttcc atggacaggc tgcggatcgc
     2041 gctgccgcca aactccatgg cgtagccggt gccaccggcg gtgccgatgc gaccgatgat
     2101 ggccagcacc acgtccttgg cggtcacgcc gggaccgagg gcgccatcca cgcgcaccag
     2161 cagggtcttg gacttcttct gcagcaggca ctgggtcgcc atcacgtgct ccacttcgga
     2221 cgtgccaata ccgtgggcca gacaggcgaa ggcgccatgg gtggaggtgt gggaatcgcc
     2281 gcacaccacg gtcatgcccg gcagcgtggc gccgttttcg gggccgatca cgtggacgat
     2341 gccctgacgc tcatgcatga aggggaagta ggccagcgcg cccacctcgc ggatgttggc
     2401 gtcgagggtt tccacctgct ggcgcgagac cggatcctgg atgccctggt cccagtggtc
     2461 ggtaggtgtg ttgtggtcgg ccgtcgcgac gatggaattc acgcgccagg gcttgcgacc
     2521 ggccagcttg aggccctcga aggcctgcgg gctggtgact tcatggacca gatggcggtc
     2581 gatgtagatc agggcggtgc cgtcgtcctc gacgtggacc acgtggctcg accagagctt
     2641 ctcgtagagc gtttgcgcgt gcatgggcgg ataaagcgac ggaaaagttt cgattatttc
     2701 acaggcttcg atccctgagt aggccgggcg ttgcgggcct cctcgtagaa cggcatgacc
     2761 cgcgccgcgt actgctgcag atcggcctgg cgggtttcgt tggacgggtg ggtggacagc
     2821 cactggggcg gcgccccgtt ggacaccttg gccatcttct gccatagcac gatggcagcc
     2881 cgcgggtcgt agccggcgcg ggcggcgagc tccacgccga tgcggtcggc ctcggtctcc
     2941 tgctcgcggg aattgggcag cccgaaggac acgttggcca cctgcccgat caggttggtg
     3001 ccggcctgcc cggcacccag cacggcgccg acgatactca cgccgatgtt ggtcaccatc
     3061 gcctgggatg cccgctcacg ggcgtgctcg cgcagggcgt gggcgatctc gtgacccatg
     3121 atcgccgcca tttcgtcgtc ggacagctgc aggcgctcga tgatcccgga gtagaaagcg
     3181 atcttgccgc cggccatgca ccaggcgttg agctcctctg acgtgagcac gttgacctcc
     3241 cagcgccagc ccggcgcatc ggggcggaag gcgccggtgg ccggaatcag ccgacgcgcg
     3301 acgacgcgca ctcgctccac ctggacgcgg tcccggttca gggcgttctt ctcggccgcc
     3361 tgctggagga tcttgtgata gctctgcgcc gacgcctgct ccatttccct ggacgacacc
     3421 agcatcgact ggcgccgttc gacgcccacc gccccggact gggtcgtctg caccgtctgg
     3481 caggccgtca ccatcaagac tgctgccccc aggggcaaca tccccttcca cacgctcatc
     3541 aaagccatac ccactccttg tcgagttcaa gcctcgcgga ccgccggtcg ccagcccgcg
     3601 agcaggaccg ccaggcccat caacgcaaac gcggacccga gggtgtacgt ccaccctgcg
     3661 ccgatgctgt cccatgtgta accactcacc acaccgccca ccatgccccc cgcgccgaag
     3721 gacacactgc cgtagagcgc ctgaccgcgc gcctggagtt gcggcccgaa ccagcggttg
     3781 atcgccgcca cgcccgccgc atggtaagcc ccgaacgtgg ctccatgacc gatctgggcc
     3841 aggatcagca cggccaatga attggctccc cagccgatca gcaggaaacg cacgaccgcc
     3901 acggcaaagc tgaacagcag gatgccccgc agggaatacc gccgcaggat ctggggcatc
     3961 gccatgaaca cggcgatctc tgccaccaca ccgagggtcc acatccagcc caccagagtc
     4021 ttgtgatggc cgagctgcac cagatggacc gaatagaaga cgtacagcgc accgtgagcc
     4081 gccgacatga agaaacaggc ggtcagcagg gcacgcactt ccctgcggcg gagcacctcg
     4141 cccagagatg ccgggttcat tgaatgggcg ggtgccgcgc cttccggcag cacggccgca
     4201 tagaccagaa tcccgcccag cagcccgagg gtgatccaca gcagcgtgcg gatcggcgca
     4261 ccgtcgagca cataccccag gcccagcacc gcgagaatga agcccaccga gccccagacg
     4321 cgaatgctgc cgtagcggct ggcgtgggca cccaggtggg aaaaggtcaa ggcttccacc
     4381 agcggcaaag ccgcactcca gaaaaaggcc agcaccgcca tgctcgcaaa cagcccgacc
     4441 ggcgattcgg tcatcagaaa gcccgcaaaa cccgcaagac tcagcaccgc cgacaggcgc
     4501 acgatgggca cccgcgcgcc cagccgatcc gccagccagc cccacaagtt gggcgccacc
     4561 agacgcatca ccgacagcag ggacatgagc agcgcgatct gcgccgccga gcggccgatg
     4621 gactgcaggt aaaggctgaa atacggcgag aaggcgccaa cgaaggcgaa atagaagaag
     4681 taatagcccg acaggcgcca gtacgggatc atgggcaggg cttgtggtgt cggaagaatc
     4741 gggcgctcgg ggcattcaaa gggcggcagg acaaaaaaag agcgccctcg ggcgcccttt
     4801 tcagcggagc tgcagcggtc agtttactac ggcgcctttc agcatgtcgt agagttcatc
     4861 cttcagcttc agacgacgct tcttgagctc ttccagttca aaatccgtcg ccagttcggc
     4921 attcagctct acccggacca cgtggcgatt cacgtcgtga tattcgtcga acagacgtga
     4981 gaaatggttg ttcgacacct tgagcgaatg aatctggtcg cggtactcgg ggaattcgtg
     5041 attcagatcg tgttgctcca tgcaagacct cccggtggac tgttttcagg tagatgacag
     5101 gctctgaggc cgactgtacg cccctgagtc ccccgaaaag ttgatggacg tcaacccttg
     5161 gaacccttgg gcgagggcac gccctgcacg ccgtcgggcg ccagacccgc caccggcggc
     5221 gtgggacagg ccacatccgc gttctgggca cgatggcgca gggcatgatc catcagcacg
     5281 atggccagca tgctctcggc gatgggcgtg gcgcggatgc ccacgcaggg atcgtgacgc
     5341 ccgtgggtat tcaccaccac cggctcgccc gccttgttga tggagcgccg gtccaggcgg
     5401 gtgctggagg tgggcttgat ggcgatgctg acgaggatgt cctgccccgt tgagatgccg
     5461 cccagcacgc cgccggcctg gttggagagg aagccctccg gcgtcatctc gtccgaatgc
     5521 tgggtgccga gctgggccac gctggcgaag ccggcgccga tctccacgcc cttgacggcg
     5581 ttgatgccca tcatggcgtg ggcgatgtcc gcgtcgaggc ggtcatacac cggctcgccc
     5641 cagcccaccg gcacgccggt cgccaccaca ttgatgcgcg cacccaccga atcgccggac
     5701 ttgcgcagct cgtccatgta gtcttcgagt gccggcacga gggcggcatt gggcgcgaag
     5761 aaggcgttgt cgtccacatc gtcccaggcc gcgaagggaa tctccacggg cccgagctgg
     5821 gccatgtagc cgcggatcac gatgccgtag cgctcggcca gccacttgcg ggcgacagcg
     5881 ccggccgcca cacgcaccgc ggtctcccgc gccgacgagc ggccgccgcc gcggtgatcg
     5941 cggatgccgt acttttccca gtaggcgtag tcggcatggc ccggacggaa ggtctcggcg
     6001 atgttgccgt agtccttgga gcgctgatcc tggttgcgga tcagcaacgc gatcggcgtg
     6061 ccggtggtct gcccttcgaa cacgccggag aggatctcca cggtgtccgg ctcgcgacgc
     6121 tgggtgacgt gacgggacgt cccgggcttg cgccggtcca gctccgcctg gatgtccgcc
     6181 tcgcacaggg ccagccccgg cgggcagcca tccaccacac agccgatggc cgggccgtgg
     6241 gattcgccga aggacgtgac ggtgaacagg gtgccaaggg tattgccgga catggggcgc
     6301 tcgcgaacgg aaagaggtct caacaaacgg aaaggccggc ccgcaggccg accttgagtc
     6361 tagcacgcga ccggcagggc gccggccacg cgggtactta ctggaagaac tcgtccaggc
     6421 ggccgaacac cgggtggtca ccggcgtgct ggcggacgta gagcacgtct tccagctttt
     6481 cgacctggcg gatcatcttc tcgaggcgct catcctcgaa aatcagcagc cagatgcggc
     6541 tggtgccgcc ctcgcccacc ggcatgcaca ggatgccttc cacgttgaag gctcgccgcg
     6601 cgaagaggtt gcagatgtgg ctcatcaccc ccgggtggtt ggccacggcc agctccagca
     6661 cgaccttgtg gaagccggtg tgctcgggca cgtcggggat gtactcggac agttgggagg
     6721 tttggtcgga catcgtgttt gctcctgggg ctcagccgat catctcggag ttggcggcgc
     6781 ccggcggcac catcgggtag acgaactcgt tcgggtcgat cgtcgcgtga atcaggcagg
     6841 gccccggggt gttcagggct tccgccaggg tcgcgcgggg gttgggcgac ggatccagat
     6901 tgacggcgcg catgccgaag ccttcggcga tcttcaggaa gtcggtcgga tgctcgtagc
     6961 gggaggccat gatgcgcttg ccgtagaaga ggttctgctg ctgatgcacc agtccgaggg
     7021 actggttgtt gaacagcacg atcttcacat tgacgttttc ctgggcggcg gtggccattt
     7081 cctggatgtt catcttcagg ctgccgtcgc cggagaagca caccacggtg cgctcgggct
     7141 cggccaaggc tgcgccgatg gcggccggga agccgaaacc catggtgccc aggccaccgg
     7201 aattgagcca ctggcgggcg cggcggaagg gataggcctg ggccacccac atctggtgct
     7261 ggcccacgtc gctggtgatg atggcgttgt cgtcgagaca gctcgccacc gcgtggatca
     7321 ggccgtaggg cgtgcgcgga tcgtccacgc ccggcatggc cagcgggaag cggcccttga
     7381 ggctctccac gtgggacagc cagcgcttgc gcagctgcgg cttgacccgc ggcagcagct
     7441 cgcccagcac ctgccccacg tcaccggcga tggccacgtg ggcggtcttg atcttgtgca
     7501 gttcggacgg gtcgatgtcg atgtggatga tcttggcgtt ggggcagaac tcggcggcct
     7561 tgccgatggc ccggtcgtca aaacgggcgc cgacgcagat gatcaggtcg gactcttcca
     7621 gcacgtagtt ggtgtagcgc gccccgtgca tgcccagcat gccgatggcg aggggatggt
     7681 ccatgggcat cgcgccgagg gccatcaggg tcatggtggt gggcagggag gcctgctcgg
     7741 cgagggtcac ggccagctgc gacgcgccgg agtggatcac gccgccgccc aggtacagca
     7801 ccggacgctc cgccgcctcg atcatgcgag cggcttcttc cacggcggcg atatccaccg
     7861 gcggcggcgc atcgggcacg accacgtcgg ggaagtcctc gaagctgatc atctggttct
     7921 gcacatcctt gggcacatcc accagcaccg ggccgggccg gtcggacatg gcgatgcgga
     7981 aggcggcggg gatcaccgtg agcagttcct tcgccgagcg caccaggaag ttgtgcttgg
     8041 tgatggggat ggtcaggccg taggtatcca cttcctggaa ggcatcggtg ccgatcatgg
     8101 acaggggcac ctggccggtc agggcgatga tggggatgga atccagcttg gcatcggcga
     8161 tggcggtaat caggttggtc gccccgggac cgctggaggc cagacagact tcgggccggc
     8221 ccgacacccg cgccatgccc tgggcgatga aaccggcgcc ctgctcgtga cggcacaaca
     8281 cgtggtggat cgattcgctc cgggtcaggg cgtcatacat cggcagcgcc gcaccgcccg
     8341 gcacgccggc cacggtacgg atgccctgcc gttcgagcag cttcatgatc aagtctgctc
     8401 cggtcatctg catcatcggg tgatctccta aaggctggga agccggactg cccttcccgg
     8461 tcggggtgcg ccaaaaagca aaaacccccg ccggttttgc gccggcgggg gtttgaaatt
     8521 cgtgttcgac tgcttgttcc ccttacgacg cgaccatgcc tacgctgact acgcgtacga
     8581 ctacgacgac agcaggcagg aaacgggcgt tatgggtcac ggaagggttc aggtcgaaac
     8641 ttcaggctcc ggattgtggc acaaagcgga caaaactcaa gcgctttctg caccgcacca
     8701 gggcgtgcgc cacgctccgg tgcggtgcag gttcacttcg gcgtgctcgc gtcccgcagc
     8761 gcccggcgca ggatcttgcc cacgttgctc ttgggcagct cttcgcggaa ctccacatag
     8821 tggggcacct tatagccggt gaggccctgc cggcagtggg cgatcagcgc ctccgcggtg
     8881 agcgtcggat ccttgcgcac cacgaagatc ttcaccgcct cgcccgagcg ttcgtccggc
     8941 acgcccaggg cggccacctc cagcacgccc ggatggctcg ccaccacgtc ttccacctcg
     9001 ttgggataca cgttgaagcc ggacaccagg atcatgtcct tcttgcgatc cacgatgcgc
     9061 acgtagccgt caggcgcgat taccgccaca tcgccggtgc gcaggtagcc gtcccgcgtg
     9121 aacacccgcg ccgtgtcctc cggccggttg tagtagccct tcatcacctg ggggcccttg
     9181 acgcacagct cgccgggctc gcccaacagg acttcgtagc cctcgtcgtc ccgcaggctg
     9241 acgtcggtcg acgaaatggg caggccgatg gaatgattga actcaggcag gtcgagggga
     9301 ttgatacaca ctgccggcga cgtttcggtg agcccgtagg cttccgccag gggccgcccg
     9361 gtcaccgcct tccagcgctc tgccaccgcc tgctgcaccg ccatgccgcc ccccagggcc
     9421 agccgcaggg aggaaaaatc cagcttctcg aaatccggat ggttgagcag cgcattgaac
     9481 agcgtattga cgccggtgat ggcggtgaag cggaccttcg ccagttcggc gatgaagccc
     9541 gggatgtcgc gcgggttggt gatgagcagg ttctcggcgc cgatcttgaa gaaggtcagg
     9601 cagttcgccg tgagggagaa gatgtgatag aggggcagcg ccgtgacgat cagctcctcg
     9661 ccctcccgca cataaggccc gatccaggcg tgggcctgct gcaggttggc gacgatgttg
     9721 ccgtgggtca gcatcgcccc cttggccacg cccgtcgtgc cgccggtgta ttgcaggaag
     9781 gccagatcct cgtgccccag catcaccggc cggaggggat gggcggcgcc cttcgccagg
     9841 gcctcgcgga aggcgatgct gccgggcaga ctccacgccg gcaccatctt cttcacgtgg
     9901 cggaccacga aattcaccag cagcgacttg ggaaacccca gcagatcgcc gagcccggtg
     9961 gtcaccacgt ggcgcaccgg cgtgctgggc agcacatcct gcagggtgtg ggcgaagttc
    10021 tcgacgatga cgatcgcctc gcagccggcg tccttgagct gatgttccaa ctcccgcgcg
    10081 gtatagaggg gattgacgtt caccaccgtg agtccggccc gcagcacgcc gaacatggcc
    10141 accggatatt gcaggcagtt gggcatcatc agggcgacgc gggcgcccgg cccaaggccc
    10201 agttcgccct gcagccagcc ggcaaagcgc gcggacagct cgtccagttc accataggtg
    10261 agggacttgc ccatgttcac ataggcggtg cgcgctgcaa acttgcggac gctctgctcg
    10321 aagaggtcga cgagggacga gaactcgtcc aggtccacct cggggggcac gccagggggg
    10381 tagctttgca gccagatctt gtccatgttg tctccttagg tcgttggttg ttttaagcct
    10441 gaccaccggg cccggccgtc gcagtgcgac cggtcatcct gcggtccgcc ctcccgaggc
    10501 ggccccggtg ccttgccggc accggcttcc aacgtacgtc cagatcggcg cgtgggcgcg
    10561 ccttgtcatg acggatccgc gatcatgcgc agatcccgcc ggcccgtccg ccccctgcgc
    10621 ggtgcgggcc ggagtgccgc tcagacctgg ccttcttccg acggccagtt gcggatgtag
    10681 ttcttgagca tgcggttctc gaagctctgc tcttccagca cggccttggc cacgtcgtgg
    10741 aaggacacca cgcccatcag catggtgtcg tccatcaccg gcaggtagcg ctggtggtgt
    10801 tcgaccatct ggcggcgcag ttcatccatt tccatgctgg gattggccac gaccggagtc
    10861 ttgagcatgg cttccgtgac cggcagggac tcccagccgg cgccggcctt ctgggtggcg
    10921 acgagcactt cgcggaaggt cagcagaccg gccatgtggc ccttgtcgaa gaccaccagc
    10981 gagcccacgt cctgctccgt catcaccgcc accgcatcgg cgagggtctt gttggccgcg
    11041 atcgtgaaga gcaccttgcc cttgatcgcg agaatttcac tgaccagcat gttcttgttc
    11101 ccctagaagc gcgaacgcct tgttttgtat tgcacgcgat ggtattcgat tgtggagccg
    11161 gcgagaagcc tgctgcgaca cggattcagg cagcatttcc cgcctcgtcg acggccacct
    11221 cgagcgcctc gccaccggca aacaccagga cggcgcgcag cgggcgtcct ggccacaacg
    11281 ccgccaccgc ccgaccatag gtccggacct gggcccggta gacgtccagc aggcgctcgt
    11341 ccggcgcacc ggtcttgtaa tccaccaccc acagcgtttc gccgtcatcc accaggcggt
    11401 cgatacgtcc aaggcggcca gcggcgtcca caaactcgac ttcgttgcgc gcccagcgac
    11461 aggcggccgg gtcgaagaaa cgcgccagct gcggccgggc gacaagacgt ccggccagtt
    11521 ccacggccgc agggtcgagc ccggcggtgg agcgcgcttc agtgagacat tccagagcag
    11581 tgtggaaggc tgttccaaac agtgtgccgg ccgattcggc gacggcgtcg gccacgctgc
    11641 ggtactggcc gacaggcacg ggcgcaattg tcggcgccac ctcagccgcc actgcaggcg
    11701 ccggcgcagg ggccgccgcg acctccaccg gcggagagcc ccaggtgccc gcctccgccc
    11761 cgagccgccc caacgcggcg gcaatgcgct gatacggatt gggcgcggcg gcgtcccggg
    11821 tcggcaggga tccactggcc aggaaatact ggctcgcccg ggtgatcgcc acgtagagga
    11881 ggttcagatc ctcccgtgcg cgggcctgct cctcggcctc gaacagggcc gcccggcacc
    11941 ggccgcgctc gtccttccgg gtggccaggg agaaatgccg cgggcggatg tcgccgggcg
    12001 gccagtcgag cagcacctgg tcggtgtcgg gcttctggtg gccgttgcgg gcgtcgatga
    12061 gccacacgac gggcgcctcc aggcccttgg cgccatggat ggtcatgatg cgcacgcgcc
    12121 cgtccgcatc gtccccgtcg ccggggatca gcccctcgtc cggcgcctcg tcgtcgcccg
    12181 caccggacaa gcggcccagc tcgtcgatga agcgcggcag gctggggtag cgcccaccgt
    12241 ccagttccag cgccagggcg atgaaagcct ccagattggc acgcaccccg gcggccatgg
    12301 gcggcggcac cacggcgtga tagcgggcca gcacctggcc ttcgtgaaag atccggtcga
    12361 gcagatcatg caccggcagc gtcgcggcct gcgtcttcca gtcggccagc cgatgcccgg
    12421 cgcgctccag tgccgcgtcg gccgcccctt ccgccagcat ggcgccaagg cgcgcccacc
    12481 agccggcttc gggacgggcc gccagacgca gcagcgcgtc atcagacacg ccgaacaccg
    12541 gcgagcgcag ggcatgcacc agggccagat cgtcggcagg gctcgccagc cagcgcaaca
    12601 gggccaccag atcggcggct tccagggtct tgagcaggcc gccacggctc acgctgagat
    12661 acggaatgcc ggcgtcccgc agggcccgct cgaattcggg cagcacgctg cgccggcggg
    12721 tcaggatcag cacgtcgccg tagcgggccg gacgcggacc gtgctccccc gccacctgcc
    12781 agcgtccgac cagtgcggag atgtgttcgg ccaccgccag cgcctcacgg cgacggcgca
    12841 ggttttcggc gaggaccggc gccacccgca ggggatcgcg cagcgcgtcc gtcgcaacgt
    12901 ttgacgcggt cccacccgcc gcctcatcgt cctcgatcag cggcagcaga cgcacctgcc
    12961 cgggcagacc ggcacgcacc gccgtctggg gacggaagcc gacgaaagcc ggctcatggg
    13021 cgaacaggga attgaccacc tccaccacgg ccggcgcatt gcggaaggtg gcgtcgttgg
    13081 cgaggcggtc cgcgtcgaag cgccgggcga gccattcggc ggcgtgggtg aagatgcgga
    13141 aatcggcacg ccggaagcgg tagatggact gcttggggtc acccaccatg aagatccgcg
    13201 gcggcgtgcc gtcgagcccg taggcgtcca gccaggccac caggatgcgc cactgcagcg
    13261 ggttggtgtc ctggaactcg tccagcagga tgtggcggta gcgggcatcc agccgcgcct
    13321 gcaggaaggg cgcctgctcg ggatcggaga gaagccggtc ggtgtgccat tccaggtcgg
    13381 caaaatccat caggcggcgc tccgccttgt gggcctccag ggcgtgcagc aggccgcccg
    13441 cagcgctcag cacgtcccgg ttgaggacga agatgcgctc ctccagcacc gcatcggcgg
    13501 cggccaggat ctggtccgtg aggcgtccgt ggaggtcgag gaagcgcgtc tgcccggcct
    13561 cgcccaggcg ttttgcctgg gccgcggagg ccttgacggc gcggggctcg cccttctggg
    13621 tgaggaccgc tgccttgagg gcggcgaagc gcgtggcagc atcgtccgcg gcaaagccgg
    13681 cccgcagggc agcggcgcgg gcccggtcgc tgtcggtgtt ggcgtcgagc agacgtgcca
    13741 gcgcctccac cgcctccgcc agccccgccg tcgcgaggaa ggccggcagc gcggcccccg
    13801 gcgcgaccac gcccagatcg gcctgcaggc gcgccagggc cacatcgagc ccgtcgcgac
    13861 cgtcgtccag gtaggcgtgc cactcggcgc gacgattcac gaaggcctgg agcagcgccc
    13921 gcgtgcgtgc ggcgcccacc gtatcgaaca ggcgcagcag ggcggcggcc tcgacgcccg
    13981 ccggctcggc cgcgcagctg cgggcgaagc gtgcccaggc ctcgtccagc agcggcgcct
    14041 cggcctcgct gaggcggaag cccgccagcc cgctgccgaa gggcgcaccg ccgacgatgc
    14101 gggcgaacca gccatggaag gtggtgatgg tcatgggcgg ctcggccccg agcacggctt
    14161 ccagcaagcc gcgggcgcga ggcaacgccg cctgggccgc cgcggcctcc agtccgcgct
    14221 cggacaggaa gtccagcacc gccgcatcgg gcgctccggc gagcaggcgc agccactgac
    14281 gcaggcgctc ctcgatctcg cgggcggctt tgcgggtgta ggtgatggcc aggatctcgc
    14341 cgggccgcgc gccggccagc agcaggcgca gcaggcgcga caccagcagc caggtcttgc
    14401 cgctgccggc gcaggcctcg accaccacgc tgcgggcagg atcgagggcg cggcgggcac
    14461 gctcgacggg cgagtcgtgg ggcacgctca tgcctggtaa tccttgcggc acaggccgcg
    14521 catctcacaa tgtgtgcagg cgacgccgtg ggccggcagc ggtgcaccgg cgtgcagggc
    14581 atcgaaggcg gtgacgaggc gttccgcctg gacttcggcc atcgccacgg gatcgtccag
    14641 cggcgcggcg ccgacggccc cgtcgtcgag ggccacgtag gcggcctcgc ggacggccgt
    14701 gccacgcagc agggtataga ccgccagttg cacgtcgtcc acgtcggcgg cctgcttgcg
    14761 cagcgcaccg aggggccgcg acttgtaatc cagcaccgcc tcggtgccgt cccggcgccg
    14821 gtcaatgcgg tccaggcggc cgtggagggt cacgcgggcg ccgtcggcca gggcaaagtc
    14881 gcgccaggcg ctttcctcgg cggcagcgaa ggtccagccc tccgcctccc gcgccttctg
    14941 ccaggccacg taggacggca gttgcgcccg ccagcgggcc gcccaggcgt gttcgaggaa
    15001 gttgcgggcg agcgccgggc cgaacacgcg cgcgctctcg tcctccagcg cggcgacgag
    15061 gaccgcgtca tcctccgtcg cgatctgcgg gaagcgggcg tggaaagcct gcaggatggc
    15121 gtggatcatc tggccgtaat cggccttctc gagggcctcg ctcacctcct ccgcctcgcc
    15181 caggcccagc acgtggcggg cgaagtagcg gtaggggcag gcgatcaggc tggccagacc
    15241 gctggcggag atgcgcgtgg gaacctgctc cggcatgagc acgggcgccg gaacaggctg
    15301 tccgacgacg ggcacgtcgg cttcgaacga ccctggcacg ggcgcacgca gggactcgcc
    15361 gaaagcctgc tggtgcacca gggacagcac atcgaccgcc gcacagggca gcacggcttc
    15421 gccgtcgcgc aggtgctgcc agctgatgca caccgcgtcg gccagcgtga ggagtccggc
    15481 caggtcgtca tggagtcgcg cccgcgcctg ggccgatccg ggcaggccca gctccgcgcg
    15541 cacgccttcg tgggcgaaca ccgggcggcg atcctgcgga gccaggttgt cggcgtcggc
    15601 gccgatgagg gcggccgcct cgaagcggcg caggcgggcc gccgccagat gggtcatcac
    15661 caccgggctg tccacgctgc ggtcgcggaa catcacgctc tccagctccc ggtcaagcca
    15721 ctggcgccac tcgtgaaaac cgatgcgcgc gtcctcgtcc gccagttcgg cacggcgggc
    15781 ggcgatccac gccaggatgt cggcccctgc cgcatcggat gcgagggcgg tcaccgcacc
    15841 cagggccgcc agcgccgcac ccagccgatc gagccactgg gccggcgtcg ccgcggtgcg
    15901 gggaaacacc gaacgcgcct caagcagcag gcgcaccagg gccgcggccg gggcatcccc
    15961 cgccgccagc agggccacgg catccaggcc ggaggcaagg ccatgatggg cgtacaggcg
    16021 cgccaccgct tcgatgcccg ccgccctgtc ggcctcgccg gtgctgcccg ccacgaaggg
    16081 cgacttgagc agatccagca cgtcccggtg atagccatcg gacgacagca ccgacagcca
    16141 cgcgtcgatc agggccgcgg cgcgggtggt ggacagcttc cagccagtct cgtcctccac
    16201 caggacgccg tcccgctcca gcagggcgcg ggtgcgccgc gcggcgaccc ggtcagccgc
    16261 caccagggcg atgcgccgct tgcccgacgc cagccagccg cgcaccgtcg cggccacggc
    16321 acgggcttcc tgctccaggc tgtgggccgc gaagagacgc acgcggccgc ccagtggcag
    16381 gccggtgtcc gccagcccgg ccgcccgcag cgccagaggc ggcgcatcgg cccctgccgg
    16441 ccaggccgcg ttgaggacac gggcgagacc atcggtggcg cgccaccgat ccggcacgaa
    16501 cacggtgacc ggcacccgct cggcacaggc ggcgtagacg tcccgctcga agggcgacag
    16561 ggcgccttcg gcgatcgcaa acagcggcgc cgtccgcgcc gccgcccagg cccgggccgc
    16621 cagcagccgg gcggcggcgc gggacggcac gccccggttg tcggcctgcc acagcacatg
    16681 cacgaggcgc gcctcgaagc gcaggggctc gtccacggcc cgggcgtagg catgcgcaag
    16741 acgcatctgc agctcggcct cggtggccgg cagaccgacg ccggccaggg tcagttcgtc
    16801 gaagagggcg accagctcgg cgcagacgtc ccacaggctg gcctcgtcca gccagcggcg
    16861 ggccttgagg gcgtggtaga gcgcaaacag ccggcgggaa tcgggcagat ggggctgcgc
    16921 cgcgagccag ggctcgagca ccgccggcag ggtctgcagg cgcggcagca ggaccgcccc
    16981 gcccgccgcg gcgtggaaag cggctcgcag atcactggcg agcagcaggt tgggcaggat
    17041 caggccggcg cgggacagct cggccggatc aggggccgcc gccagcacat ggccggcaca
    17101 ggccttgagg aaatcccccc cagaggggag ggtgacgacg gtcacatcgc catgcaccac
    17161 ggtgccgtga cggaccgccg cggacgctca gcgttccaga tattgcagct tgtccggcac
    17221 accgcgccag tcgtcggcat cgggcagcgg atccttgcgc tcgacgatgg gcgtccaggt
    17281 cagggccagc tcggcgttga gggcgatgta ggactcctgg ccggcgggca cgtcatcctc
    17341 ggcaaagatc gcctcagccg ggcattcggc cacgcacagg gtgcagtcga tgcattcttc
    17401 cggatcgatc accagaaagt tggggccttc acggaagcag tccaccgggc acacgtcgac
    17461 gcagtcggtg tatttgcact tgatgcagtt ctcggtaacg acgtaggtca tgacgttctc
    17521 tctccttggt ggcccccgcc ggtgcgccgg aaaggcggcg accgacggca gaaaatcagc
    17581 gtattcaggg gccggaatca taccccattc ggcgttcctg ccccatcccc ggcacgcgag
    17641 cggcgccgct gtgccggcga tgccgccctc gccgtttcgc tcccctcgcc cgaaaaccgc
    17701 ttttcttgcc tgccaatatt cgctaggatc acctccatcc atttcattgc gcgggcggct
    17761 ccgtcctaag cgagcccgga agccctcttc cctcccggca atctccttca aaaatcccct
    17821 gaactgcgag gaatcatgag cgaacatatc cattacgtca ccgacgcgaa tttcgaagcg
    17881 gaggtgctcc agtcccagac ccccgttctg gtggactact gggcggagtg gtgtggtccc
    17941 tgcaagatga tcgccccgat cctggacgac gtggccaagg aatacgacgg caagctcaag
    18001 gtcgccaagc tcaacatcga cgaaaaccag gacaccccgg ccaagttcgg catccgcggc
    18061 atccccaccc tgatgctctt caagggcggc aacatcgaag ccaccaaggt cggcgccctg
    18121 tccaagtcgc aactcacggc tttcattgac agccacctct gagccggtta cattccgtag
    18181 cgtcagtctc ctgcgacgtc cgcggagcgg ccccacccgg tcccgctccg ccgtcgcggt
    18241 acagcaagag gcagtcctgc ccctgtacct gcagccgtaa cggctgccca ttcttcccgc
    18301 ctccttcccc ttcttctctc cccctccgca cgccatgcat ctctcggagc tcaagtctct
    18361 ccacgtcggg caattgctcg aaatggccat cgccaatgaa atcgaaggcg ccaaccgcct
    18421 gcgcaagcag gagctggtct tcgcgctgct caagaaccgc gccaagaaag gcgagccgat
    18481 cttcggcgac ggggtgatgg aagtgctgcc ggacggattc ggcttcctgc gctcgcccga
    18541 cacctcctat ctggcgggca ccgacgacat ctacgtgtcg ccgagccaga tccgccgctt
    18601 caacctgcac accggcgaca cggtggaagg cgagatccgc acccccaagg acggcgagcg
    18661 ctactttgcg ctggtcaagg tggacaaggt caacttcgag ccgcccgaag cctccaagca
    18721 caagatcctc ttcgagaacc tcaccccgct gcaccccaac aaggtgctca agctcgaacg
    18781 ggacatccgc ggcgaggaga acatcacctc ccgcgtcatc gacatgatct cccccatcgg
    18841 caagggccag cgcggcctgc tggtggcgcc gccgaagagc ggcaagacgg tgatgctgca
    18901 gcacatcgcc cactccatca ccaccaacca tcccgacgtg gtggtgatcg tgctgctcat
    18961 cgacgagcgc cccgaggaag tgaccgaaat gcagcgctcc gtgaagggcg aagtggtcgc
    19021 ctccaccttc gacgagcccg ccatccgcca cgtgcaggtg gccgaaatgg tcatcgagaa
    19081 ggccaagcgc ctggtcgagc acaagaagga tgtggtgatc ctgctcgact ccctgacccg
    19141 cctcgcccgc gcctacaaca ccgtggtgcc ggcctccggc aaggtgctca ccggcggcgt
    19201 ggacgccaac gccctgcaga agcccaagcg cttcttcggc gccgcccgca acatcgagga
    19261 aggcggctcc ctcaccatca tcgccaccgc gctgatcgac accggcagcc gcatggacga
    19321 cgtgatctac gaagaattca agggcaccgg caacatggag atccacctgg atcgccgcat
    19381 ggccgaaaag cgcgtgtatc ccgccatcaa cgtcaaccgc tccggcaccc gccgcgagga
    19441 gctcctcctc aaggccgacg tgctccagaa ggtgtgggtg ctgcgcaagc tcctctacgg
    19501 catggacgac atcgaggcga tggaattcct cctcgacaag atcaaggcca ccaagagcaa
    19561 cggcgaattc ttcgacgcca tgcggcgcgg ctgatccgcc cccggctcag gccccatcga
    19621 cgccacctcc gggtggcgtc gtgttttccg ggcggcatcg gcacaggccc gacgaaacac
    19681 tggtcgcggc cctgccccgc cagtacaatg cgcccctcct gcggacgtgg cgaaattggt
    19741 agacgcacca gatttaggtt ctggcgccgc aaggcgtggg ggttcgagtc ccttcgtccg
    19801 caccatgaaa aaaccggccc tctggccggt ttttgtttgc gcctcgcaga cgcctcaggc
    19861 cgccgcccgg cacaaggcct gatgccgctc gaacaccgcc accaggcggt cgatatcttc
    19921 ctgcgtatga cccgcgttga ggatcacgcg caggcgcccc tggcgtcgcg gcaccgccgg
    19981 aaacttcacg ctgtccacgt gcaggccggc ctcgtgcatg cacgccgcca gatgatcggt
    20041 cagcgcctcg tcgccgatca gcaccggcac gatgggcgtc tcggtcgcca ccagccggta
    20101 gggcagtccg cgcatgcgct ccacgaaata gcgctggttg gcccacaggc gctgcaggcg
    20161 ctcgggctcg tcctgcacca tgtccagcgc ctcgctcacc gcaaaagcct ggtccggcgg
    20221 cagcgtggac gtgaagccgt aggccgtgca ggtcaggcgc agcaggtcgc cgatggcctg
    20281 ttcggtggcg atgaagccac cgatggcgcc ataggccttg ctcagggtgc ccatgcagat
    20341 cagacgcggg tcgtcgatcc cgaaatgttc cgtgatgccg cccccgtggg cgcccagcac
    20401 gccgctgccg tgggcatcgt ccacatacac catcgcgtcg tagcgctcgg ccagggccag
    20461 cagctcgggc agcggcgcga tgtccccgtc ctggctgaag accccgtcgg tgacgatcac
    20521 ccggctggtg gccgcggact ggttgagcag atgctccagg tgtgccagat ccaggtgccg
    20581 gtaggtgaag cggtcggcgc cggacaggcg gatgccctcg cggatgctca cgtggttgta
    20641 ttcgtcactg aagaaggcat aggtgtgacg cgtgcgcggg cgatagccat aggtccgggc
    20701 cacctggccg atgcgcggca gcgcggcgag cacccccagg ttggtcagat accccgtggc
    20761 aaaaagcagc gccgtgtcct tgcccttgag ccgggccagc ttgtcctcca gggtccgata
    20821 gacgtccaga tcgccgccca gcaggcgcga ttcgcaattg gccacgccgt aacgctgcag
    20881 accggtcatc gccgcgcgga tcatgcgcgg gctggtggcc agcgacaggt aattgttggt
    20941 gctgaaggag acgtgctcgg ccccgtccat ccggaaatgt ggcgccggca gggattcgac
    21001 gaggggcacc gcatggacgg caccacgcgt cccgagccag tcggcaattt ctgcaaattc
    21061 tttcatcgtg cggggccgcc catggacggc agggataagc gagcgcccga tcttagcgac
    21121 agacgctgtc agcaccttgg ctccggtctg acgccgggct gtcacgctgc ccatcctggg
    21181 aagtcccccg cccgggcccc cttccggtat cctgcggcgc gcctgcgcat catcgcggag
    21241 gcccctgccg ccggaccgac tccatgctca ccgccctgcg caccctgccg tccaccgtct
    21301 ggctcctcgg cctggtcagc ttcttcaatg acaccgccgg cgagctggtc tatccggtgc
    21361 tgcccctcta tctcgcctcg gtgctgatgg ccggccccag ggccctcggg ctcatcgaag
    21421 gcatcgccga ggccaccggc agcctgctca agctggtctc cggcgtgctg tcggaccgcc
    21481 tgcgccacac caagcgctgg gtggtggccg gctatgccat cgccggcctg tcgcgccccc
    21541 tgctgaccct cgccaccagc tggccgctgg tgctcgccct gcgctttgcc gaccgcatcg
    21601 gcaagggcct gcgcagctca ccgcgggacg ccctcctcgc cgccaccgtg gcgccgcaac
    21661 accgcggtct ggccttcggc ctccaccgcg cctgcgacaa cgtgggctcg gtggccggtc
    21721 cgctcctcgc cgccgccctc ctcgcccgcc aggtgcccct ggaacggatc ttcctgtggg
    21781 ccagcctggg cggcgcggtg gccgtggccc tcaccctgct gatccgcgaa ccggtccgtg
    21841 tcgaatcccg cccggcgctg cgccccggct ggaccctcgc caccctgccg ccggcgttcc
    21901 ggcgctatct ggcggtgctg gtgctgttca ccctgggcaa cgcctccaac atgttcctgc
    21961 tcctcaagag ccgcgacgcc ggcgtggccg actaccaggt gccgctgctg tgggcgctgg
    22021 tctccttcac cgccgcgctg ttctccacgc ccctctcggc cctctccgac cgcatcgggc
    22081 gcagccgcct gatcctcgcc gggtggagcg tctatgccct gttctatctg ctcctcggcc
    22141 tcgccggtca ggcctggatg atgtggcccc tgttcgcctt ctacggcctc ttcatggccg
    22201 ccaccgaagg ggtcgagaag gccctggtgg ccgacctggt gccgcaggaa cagctcggca
    22261 ccgccttcgg ctggttcaac ctggcggccg ggctgaccct gctgccggct tcgctgatct
    22321 tcggcgccct gtgggacgcc ctcgccccca ccgcggcctt cgccttctcg gccgcctgtg
    22381 ccgcgctggc cgcgttgctg ctgcgcctgt gggtgcaccc ggaacgaccc tgaggcgtcc
    22441 ccgccgccgc cccaccccct gcgcagtcct gcgcattgtg ggcgtccgcc cacctgcggt
    22501 ataagaaccg ccacccatgc ccgaccagca cgcccctccc gtccagcgcg tcgtcaagat
    22561 ccggcgcgac tacaacacct gggtcgcccg cgagaccctg gaggactacg ccctgcgctt
    22621 cacgccgcgc aacttccggc gctggtccga gctgcgcgtc gccaacaccg ccttcggcgc
    22681 cgcctcattt ctggtgctgg aagccgtggg cgccacgatg ctggtgaacg ccggcttcat
    22741 caacgccttc tgggccatcc tcgccaccgg cctcatcatc ttcctgatcg gcctgcccat
    22801 cagccattac gcggcccgtc acgggctgga catggacctg ctcacccgtg gcgccggctt
    22861 cggctacatc ggctcgacgc tcacctccct catctacgcc agcttcacct tcatcttctt
    22921 cgcgctggag gccgccatca tggcctacgc cctggagctg gccttcggcg tcccccccgc
    22981 ctggggctac ctgctctgcg ccctggtggt gattcccctg gtcacccacg gcatcaccgc
    23041 catcagccgc ctgcaggcgt ggacccagcc cctgtggctg gtcctgttga tcctgcctta
    23101 cctcttcctg ctcacccagg agcccggcgt gctcgccgag cttgtccgct atcccggcgc
    23161 gaacggcgat ggcggcgcct tcagccccca gctcttcggc gccgccctca ccatcggcat
    23221 cgccctggtc acccagatgg gcgagcaggt ggactacctg cgcttcatgc ccgaacgcac
    23281 ccccgccaac cgccgcgcct ggtggctggg catgatcctc ggcgggcccg gctggatcct
    23341 gcccggcatc gccaagatgc tcggcggcgc cctcctcgcc tacctggccc tgcgcaacgc
    23401 ggtgcccgcc gacaaggccg tggatcccaa ccagatgtac ctgatcggct tctcccacgt
    23461 cttcgaccac accggcctgg ccatcgccgc caccgccctc ttcgtcgtcg tctcccagct
    23521 caagatcaac gtcaccaacg cctacgccgg ctccctcgcc tggtccaact tcttcgcccg
    23581 cctcacccac agccaccccg gccgggtcgt gtgggtggtg ttcaacaccg tcatcgccct
    23641 gctgctcatg gagctggacg tcttccaggc cctcggccag gtcctcggcc tctactccaa
    23701 cattgccatc tcgtggatgg ctgcggtggt ggccgacctg gtcatcaaca agcccctggg
    23761 cctcgccccg ccgggcgtcg aattcaagcg cagccacctc tacgacatca accccgtggg
    23821 tgtcggggca atggggctgg cctccctgct gtcggtggcc accttcgccg gcgccttcgg
    23881 cgcgaccctg caaccctatg cctccttcgt cgccctgggc gtcgccttcg tcaccgcgcc
    23941 cctcatcgcc tgggccaccg gcgggcgcta ctacctcgcc cgcccggcgg ttgccaccgg
    24001 tgccggcctg caacgctgcg tcatctgcga gcgggactac gaaggcgagg acatggccca
    24061 ctgccccgcc taccagggtt ccatctgctc cctgtgctgc tcgctggatg cccgctgcca
    24121 cgaccagtgc aagccccacg ccagcgtcgc cgagcaatgg aaggccctgc tccatcacct
    24181 cctgccccac tccgcccgcg cctacatcga caccggcctg gggcactacc tgctcctcat
    24241 ggccggcgtc accgccctgc tggccctcgt gctgggggtg ctctataccc acgaagccct
    24301 gctcctcggc ccggaccaac ccgacctcgt cgcctccctg cgggccagct tcctcaagct
    24361 cttcgccgcg ctgctgctgc tctccggcgt gggcgcctgg tggatggtgc tcacccagca
    24421 gagccgccgg gtcgcccagg aggaatccaa ccgccagacc gaactcctca tgcaggagat
    24481 cgaatcccac cgccgcaccg acgaacagct ccagcgcgcc aaggaagccg ccgagcaggc
    24541 caaccaggcc aagagccgct acatcaccgc catcagccac gagctgcgca cgcccctcaa
    24601 cagcatcctc ggctatgccc agatcctcgc cgccgacgac gccatccccc cgggccggcg
    24661 ccaggccgtc agcgtcatcc gcaagagcgg cgaccacctc ctgtccctca tcgaaggcac
    24721 cctggacatc gcccgcatcg aaggcggcaa ggtcgcgctg gaagtgcgcc cgctggaatt
    24781 ccccgccttc gtccagcaga tcgtcgccat gttcgagctg caggcccgca acaagggcct
    24841 ggacttcacc tacacccccc acggcgcgct gcccgccgtg gtccgcgccg acgaacgccg
    24901 cctgcgccag atcctcatca acgtgctcgg caacgccgtc aaattcaccg cccgcggtgg
    24961 cgtcgaactg gccgtgagct accgccgcga gatggccacc ttcgacatcc gcgacagcgg
    25021 ccccggcatc cccgccgccg acctggagcg catcttcgaa cccttcaccc gcggccgcac
    25081 cggcggctcg ccgggcagcg gcgtcggcct caccatcgcc cgcatgctca ccaccctcat
    25141 gggcggcgag ctgcagctca gcagcactcc gggcgaaggc agccagttcc gcatccgcct
    25201 gttcctgccc cagctccaca ctgcccaggc cgaggccctg ccccgcgccg cccgggtcgg
    25261 ctatgtgggc gtgcgccgac gcatcctcat cgtggacaac gaaaaggtgg accgggacat
    25321 gctgcagagc gtcctcgacc ccctcggctt ccagatcgag caggtgggca gcggcgaaga
    25381 atgcctcgtc gccgtgcccc gcttcgcccc ccacctgatc ttcatggacc tcgccatgcc
    25441 cggcatcgac ggctgggaga ccatccgccg cctgcgcgcc ggccccgcgc ccctggccgg
    25501 cgccgacgtg aagatcgcca tcctctccgc caacgccttc gacaagggcc tcgacaacga
    25561 cgccggcgtg gcgccgcaag acttcatcgt caagcccctg cgggtggacg ccctgctcga
    25621 ctggatcggc cgcagcctcg acctggagtg ggtccacgcc gacagcccgc cgccccccgc
    25681 gccggccccg gccgccccgc cgcccctcat cgcgccggac gcatcccacc tcgccgccct
    25741 ggacgaactc atcgccctgg gctacctgcg cggtatcctc ggcaagctcg ccgagatcga
    25801 acgcctcgac ccctgccacg gcgaattcgt ccgcgtcctg cgcgaacagg cccgccagtt
    25861 ccagttcgac gccatgaaag ccatcctccg caaggcccgc gatgaccgcc cttgaccgca
    25921 gcatctccga catcgtcctc atcgtggacg acgtgcccga gaacctcgcc ctgctccacg
    25981 acgccctcga cgacgccggc cacaccgtgc tcgtcgccac cgacggcgaa tccgccctgc
    26041 tgcgcgcccg ccagagcctg cccgacgtga tcctgctgga tgccgtgatg cccggcctgg
    26101 acggctttga agtggcccgc cggctcaagg ccgacttcac cacccgccac atccccatcg
    26161 tcttcatgac cggcctcacc gagaccgaac acgtggtcgc cgccttcgcc gccggtggcg
    26221 ccgactacgt caccaagccc atccgccccg ccgaagtcct cgcccgcatc gccgcccacg
    26281 tccagaacgc ccgccagatg aagcaggccc gcagcgccct ggacgccttc ggccaggcca
    26341 ccgtcgccgt gcgcgtggcc gacggccgcc tggtctggca gacccccctc gcccgccgcc
    26401 tgctgcgcga cttctttgcc gccgccggcg aggacgcccc gctgcgtctc ctcgactgga
    26461 tccacgacgc cgagctcgcc cgccgcgaag gccgcgagcc cgtcggcctg ctcgccgccg
    26521 acggcagccg ccgcctcctc gcctccttcc acgaccagac cggcgacgcc gaatggctcg
    26581 tcgtgctgcg cgaagagaac gacgcctccg ccatcgaatc cctcatcgcc gccttccgcc
    26641 tcacccagaa agaggccgaa gtcctctact gggtcaccca gggcaagacc agcaaggaca
    26701 tcggcgacat cctgggcagc agcccccgca ccgtgaacaa gcacctggaa cacgtcttcg
    26761 agaagctcgg cgtggagacc cgcaccgccg ccgccaacct ggccatgagc cggatgcgca
    26821 gcggcagcgc ctgaacgccc gccgcgacac ccggcgcgtc acgccggcgg gggtggcgga
    26881 ggccgatcct ccaggcgcga ggtggcgacc gagatctccc gcaggaaaca caccagcccg
    26941 gcgatcagcg acgccatccc gccaatgaag agcaccgcca ccagcgacga gaaatcgctg
    27001 cccagctcca cccccacgaa caacaccgcg atcaccacgc agatcagcaa ggcacacagg
    27061 gtgcaatagc ggatcgctgc atggaccagg cgcgaacgcc gctgcagcag cgccatctcg
    27121 tccgcggcgc gggccaccgc ctcggcgccg gccgccctca gcgcgcgcga acgatccacg
    27181 atgcgaccga gccgattggc cagcaccccg agcagggagc ccaccccggt caacaggaac
    27241 accggcgcca ccgactgctg gatcgcgtgg gccaccgtag gcagatcgga caccagggcc
    27301 atgactcagc gcccccccaa tccgcgacgc aaggcgccgc cgcccgccca cgccgcccgg
    27361 cccgccccct ggccagcgac ccgccacggc ccctgcaccg cctgtctgcc cttgctcctt
    27421 gcccgcgtca tcctcgccct cctgtgagtg cctgttgtcc gttccggtac cgcctgcggc
    27481 agtggccggc ctcgacccgt gccggcgcgg ggcagtctag cgaccgccgc gggccgactc
    27541 aaccgccccg gcgcccccgc ggcgccgcgc gccaacgcac ggctcgcctg cgccaggccg
    27601 ggcctcagcc ccccaccgcc acgccaaaga ggcgcccgat gccaaaagtc acgcccgccg
    27661 ccgcgccgcc gatcagcagc tggcgcgccc ccgcaagcag cgggtgcttg cccgtgaaca
    27721 ccgcaatcgc cgcccccagc acaaacagac ccagcgccga cgacaccgtg ctcgccagca
    27781 ggatggaggc ccgcggcgcc aggaacatgg gcagcgccgg aatcatcgcg cccaccagga
    27841 agaccagaaa cgacgaagcc gccgcctcca gcgccgagcc ccccagatcc tccggattga
    27901 tgcccaactc ctcccgcacc agggtatcca gcgcagagtc cttgtcgccg atcacccgcg
    27961 ccgccatctg ccgcgcctcg tcctgggaga acccccgcgc ctggtagatc aggctcagct
    28021 cctcctgctc ctcggccggc acctgggcca gctcctccgc ttcgctggcg atctgcttct
    28081 cctggagctc ccgcgcgctc tgcaccgaga tccattcccc catcgccatc gagcacgccc
    28141 ccgccagcgt ccccgccagg gccgtcgcca gcagcgcctg gggccctgct gccgtgcccg
    28201 ccacccccat caccagacag aaggtggaca ccagcccgtc attggccccc agcaccatcg
    28261 cccgcagatt gttggccgcg ccggccccgt ggcgaccttc caggcgggaa tagagcgccc
    28321 cgttccagcc cgtcgtcgag cgggacgcca gcagggacag catgcgcgcg tgggagcgct
    28381 cctgggcccg catgcccgtg cccgccgtct cccgctggtc gtcatacacc ccgcggttgc
    28441 gcacctcgtc gccggccacc aggggcagca ccgcctgggg cccgaagcgc cgcgtcgccc
    28501 acagcagaca gcgcgcccgc cagcccagcc cctggggcac cgtgcccccc gccttctcga
    28561 tgcgccgccg ccagaactcc gcgtgagtct cttccaccgt ggccagcttg cgatacagcg
    28621 ccgccacctc cgcctgggac tcctgcgccg ccatctcccg atacagcgcc gcgctctcct
    28681 gctcgctgcg gaaattcgcc agaaaccgcg ccccatcgcg ccccatcgca cccatgctcg
    28741 gtcctcccgg aaagtcgcca gtggcgcgca gatccgccgc tgccaccgtt ccgcccgatt
    28801 gtcgcagagt ccgacgcgcc cacgcctcct ccgatccccg cgacgcgctt gcacacggtc
    28861 gtcctgcaat gccgcgcacc cacctcccga cgcgccggcc cgccttcgcc cccattcacc
    28921 ccgcacgccc ggcccagaaa gcggccgaaa tccgcgacag ggtcacccag ggcaagaccc
    28981 gccag
//
LOCUS       DAOQMM010000040        28968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_461_length_28968_cov_50.072563, whole genome shotgun sequence.
ACCESSION   DAOQMM010000040 DAOQMM010000000
VERSION     DAOQMM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 28968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28968
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_461_length_28968_cov_50.072563"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLH85_08600"
     CDS             complement(<1..209)
                     /locus_tag="PLH85_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64661.1"
                     /translation="MQKGSGRNVVRSATVSVLCALLLAACGGSPESMVASAKEYLAKN
                     DRNAASIQLKNALQKNPGFGEARFLL"
     gene            complement(227..1600)
                     /gene="prsR"
                     /locus_tag="PLH85_08605"
     CDS             complement(227..1600)
                     /gene="prsR"
                     /locus_tag="PLH85_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNB64662.1"
                     /translation="MSDKRRTLLIVEDDPALQKQMRWAFDQYDTVVASNRDEAIAQIR
                     RYEPAVVTMDLGLPPAPDDVTEGFRLLGEILALMPDTKVIVLTGQHDRENAVRAVGMG
                     AYDFFAKPFEPEMLVLTLERAFRLYDLQQDNRRLKMQQGSALSGVITRDPGMLRVCRL
                     VEKVASANVTVMLLGESGTGKEVLARGLHELSPRRNERFVAINCAAIPENLLESELFG
                     YEKGAFTGAVKQTLGKIEVANKGTLFLDEIGDLPMPLQAKLLRFLQERVIERIGGREE
                     IPVDVRIICATHQDVKGLIQTGRFREDLYYRLAEIVVDIPALRDRDGDAVLLAHAFVQ
                     RFAASNGRGTMTLTDDAVQAIESHRWPGNIRELENAIKRSVIMADGNCISADDIGVGD
                     GTVEEGELEALNLRVVRDEAERRAVIRVMARVNGNVARAAEVLGISRPTLYDLMNRFG
                     LKKETST"
     gene            complement(1597..3702)
                     /gene="prsK"
                     /locus_tag="PLH85_08610"
     CDS             complement(1597..3702)
                     /gene="prsK"
                     /locus_tag="PLH85_08610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNB64663.1"
                     /translation="MELFLSQVSVWSYAFATVLHGLFALYLGIAWRRGRRGWLLVAAV
                     VLSGGWAAASWGFALTGWIWLFQLGNLFDVLRQAAWFGFLVAMLLQNASEADGNVAAL
                     AWPRPAMLAIVALAAGGVACFTLGIPLYGTPMRTWAATSLASSVFGLVLLEHFYRSVP
                     ERSHWGIKPLTLALGGLFAFDVYLFSDALLFTRLHPSVWSVRGFAHTLAIPLIAVSAS
                     RNPEWTFRISVSRHVVLHSTAMAGTGLYLTLIAGAGYYMRYFGGEWGQALQVALLFGG
                     ALFLGVVAFSGAVRSRFKVLIAKHFFAYRYDYRTEWLGVTQALSAAGDGLTLGESVVN
                     ALGDLVESPGGGVWLKDAAGRYSQQARLNMPHCGMQVGDDDAFIRFLLEKEWVVNLEE
                     ARAGVNFGESQPVPAWIGEVDDAWLIVPLISAGSLIGFVILLTPRTPVDVNWEVLDLL
                     KTAGRQAAGYFARMQAAEALLEARKFDAFNRMSAFVVHDLKNLVAQLSLMLRNAERHK
                     DNPEFQADMLDTVRHVEERMRGLMAQLMEKTPINQRKPVPLTPLLEHIVQSKRLFRPA
                     PVLVSGGPDLAVLAHSERLERIVGHIVQNALDATSEHGSVSVEVSRSGNFASIRVADT
                     GSGMSAAFIRDQLFKPFQTTKASGMGIGAYEARQYIHELGGSLSVESEVGAGSCFEIR
                     LPLAHLPGGVDGEPAAKQT"
     gene            complement(3780..5168)
                     /locus_tag="PLH85_08615"
     CDS             complement(3780..5168)
                     /locus_tag="PLH85_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNB64664.1"
                     /translation="MLKLFNHYVPSNTILQLMFDVTLLFVSVVVVFLLQTQLDESLVR
                     VVVPSALVFAITMMVLNGALGLYRAGSGADLRETTVRVLISIVLSVPVAYGIFRVLPW
                     GEFAHEAMELDVIALLGFVVAVRGFTMRKMGVGVLAKRVMVIGTGADAVAVEHALTHP
                     ANPSLQVVGFFPAGSGGDEVRVDPRRIVHYDGRLLAAANDLRVNEIIVAVKERRGGVL
                     PLRELLECKMAGIKVLDLSSFFERVRGQVRIDSLRASWLIYGEGFRQGLGRTLVKRVF
                     DIVASLILLALATPVMLLAMLLIVMEDGAPVFYRQERVGLNGRLFKVTKFRSMRRDAE
                     RDGKPRWASANDDRVTRFGRIMRKTRIDELPQLFNVLKGDMSLVGPRPERPFFVDQLT
                     KDIPFYAVRHCVKPGVTGWAQVRYQYGSSVDDAINKLQYDLYYVKNHTLFLDIVVLFE
                     TVRVVLTGDGAH"
     gene            complement(5588..7483)
                     /locus_tag="PLH85_08620"
     CDS             complement(5588..7483)
                     /locus_tag="PLH85_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNB64665.1"
                     /translation="MSAVVAEDQHGHSGTKVLPVALAAMGVVFGDIGTSPLYTMKEVF
                     NGPHAVPATPENVLGILSLVFWALTFVVSLKYVVYIMRADNRGEGGIMALTSLALRST
                     DSPRAASLMAALGLFGAALFYGDGVITPAISVLSAVEGLEVATPAFRPFVIPISLVVL
                     IGLFVIQRKGTAQVGAVFGPVMLFWFATLGVLGAFNVVRHPDVLGALNPLHAVRFFVE
                     NRVHGFLALGAVVLAITGGEALYADMGHFGRRPIKLAWFGFVFPCLYLNYLGQGALII
                     DDPSAVANPFYLLVPDPLLYPMVGLSTVATIIASQAVISGAFSLTKQAVQLGYSPRIH
                     IVHTSDREAGQIYVPNVNWMLLGAVIALVIGFKSSSALASAYGIAVTLTMMIDTVLAF
                     VVVRALWHWRWSAAVAFVALFFSVDFSFFAANSVKILDGGWFPLALGLGVHTLLMTWK
                     TGRALLTERMRVDTMPLDLFIQSMFHDPPHRVPGLGIFMTTNPDGVPRALLHNMLHNK
                     VLHASVVLVHVVTADVPYVPLEERVRLEELDFGFFRVFVRYGFKDDPDIPAALALCAE
                     KGLPYELMQSSFFLGRETLIPRKGSAMPLWREHLFNIMFRNADSAADFFRIPVNRVVE
                     FGTQIEL"
     gene            complement(7599..8978)
                     /locus_tag="PLH85_08625"
     CDS             complement(7599..8978)
                     /locus_tag="PLH85_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB64666.1"
                     /translation="MNSIDRRARASGPDVLIVDDEDDIRELLELTLMRMGLACDGAGT
                     VADAQRLLTERRYRLCLTDMRLPDGDGLDLVRFIADKVGDLPVAVITAFGSMDNAVTA
                     LKAGAFDYLSKPVSVDQLRALVKSVFSVSPADGVEGTRDTSSLGGHSAAMVQVRALIE
                     KLARSQAPVLISGESGSGKERAARMIHELGPRSGAPFVAVNCGAIPESLMESEFFGVK
                     RGAFTGADADREGFFQAAHGGTLFLDEVGDLPLPMQVKLLRAIQEKRFRRLGDTTEVG
                     VDVRLISATHRNLKSMVEASSFRQDLYYRLNVIELRMPALRERSEDIPELATTLLGRL
                     AENSGMDVPRLGPDALQALVGYAFPGNVRELENILERALALSNGNVITAADLLLEPAE
                     ELAGESPSIVVQDGGAPLQDYLDHVERQAIVEALERAGGNRTAAARALGVTFRSLRYR
                     LERLGMKDG"
     gene            complement(8975..10555)
                     /locus_tag="PLH85_08630"
     CDS             complement(8975..10555)
                     /locus_tag="PLH85_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64667.1"
                     /translation="MAYPGLESGATEFRGRWEPLRYLNLFRLVLAGVFLVAGPGLSLG
                     GDSATLFFGAAFAYLTAVLILGFPDAARHLGMERLVTVQVLVDVTLLTVIMWASGGYR
                     SGMPILIMIVLAGSGLVGQGRMVLFYAAYATVAVLFENLLRFLSGRDVVDFFTVGVTC
                     IGFFTIAQVARLLALRAIANETLARQRGADLAAQLRLNQQIIQDMQDGVLVVEPGGVV
                     RHFNPQANALLGGTLREGGALASCAPALAGPLGAGEDGEPGAHSALRFRAEGSGKLLL
                     ARMLRAGDSDATLVYLEDFDLIHDQIQQVKLAALGRLTASIAHEIRNPLAAVSHAAEL
                     LSDEKRGDMQARLIRIIGTNAQRIDRLVGDVLALGRRDEALPEVLPLKQFVDEFLDEF
                     ALTDPVRRALVDVAIASDLTLLMDRTHLRQILWNLLANAQRHGSGAPGSIRIAAGLRD
                     DGRIAVDVTDDGPGIAEALRNQVFEPFFTTHSKGTGLGLYIARELADANGAVLELGQA
                     EAGGLPGAHFRLTARSRS"
     gene            complement(10561..10815)
                     /locus_tag="PLH85_08635"
     CDS             complement(10561..10815)
                     /locus_tag="PLH85_08635"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNB64668.1"
                     /translation="MRNLLVFVLFLIGLYYVRRALQGKSGDTPGAGREARSEAPRQPT
                     EAEAMVACAHCGVHVPESEGLHVGGYFFCSDAHRRLGPRV"
     gene            complement(10844..12004)
                     /locus_tag="PLH85_08640"
     CDS             complement(10844..12004)
                     /locus_tag="PLH85_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB64669.1"
                     /translation="MPVFSAPLVSRLPTVGTTIFTRMSQLAQTCGAINLSQGFPDFSA
                     EDVLFERVAHWMRAGFNQYAPMAGMPALRDAIATKVHALYGAHYDPGAEITVTAGATQ
                     ALFTAVAALVHPGDEVIVFEPVYDSYVPAIELQGGVVRRVRLGAPEYRPDWAALRALI
                     TPRTRMIMINTPHNPTGAVWSAADMIELAAVTRGTGIVVVADEVYEHIVFDGARHESA
                     ARDPELAARSVIVSSFGKTYHVTGWKVGYVLAPQALMDEFRKVHQFNVFTVNAPVQLA
                     LADYMAEPSRHLALAAFYQAKRDAFRAGLAATHFELLPCAGTYFQTVRYAAVSDLPDL
                     AFVEWLTREVGVAAIPLSAFFGDGRDEGVIRFCFAKQEATLAEACERLARFC"
     gene            12090..13187
                     /gene="mtnA"
                     /locus_tag="PLH85_08645"
     CDS             12090..13187
                     /gene="mtnA"
                     /locus_tag="PLH85_08645"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNB64670.1"
                     /translation="MQTPSNAPVTDTCPTLRREGDSVLILDQTRLPHEEAFVRLATLA
                     DAALAIRSMQVRGAPLIGATAAYGVALALASDASDAALADADACLRTTRPTAVNLHWA
                     LDRMGRKLASVAPADRREAAWGEADAIRREDADANAAIGQHGLALLNALAAEPGRPVH
                     VLTHCNAGWLATCGHGTALAPVYAAHAAGLPVHVWVSETRPRNQGLLTAWELRRDGIP
                     HTLIADNAAAWLMAEGRVDCVIVGADRIAANGDTANKVGTCLKALAARAHALPFYVAA
                     PLSTVDRSCPNGRAIPIEARDGDEIRRIGGLDDNGRPAFVHIAPPDSPVCNPAFDVTP
                     AALIDGIVTPVGVCAAHDIGRLAGSLSGDSP"
     gene            13184..13888
                     /locus_tag="PLH85_08650"
     CDS             13184..13888
                     /locus_tag="PLH85_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNB64671.1"
                     /translation="MIENVLREALLATAQSMGSLGLNTGTAGNASVRCHDNLLITPTG
                     CDYASCTPSDMVLMAMDGTWQGHCAPSSEWRIHRDILAARPEAGAVLHAHSPFATALA
                     CHRRGIPAFHYMIARFGGTDIRCAPYATFGTQALSDGALAALEGRSACLLANHGMLVI
                     GSDLRRALALAVEFESLCAQFWRACQIGTPTLLSDAEMADVLAKFRHYGQPAQAALLA
                     DALPAAGPAASGLSDG"
     gene            13901..16993
                     /locus_tag="PLH85_08655"
     CDS             13901..16993
                     /locus_tag="PLH85_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64672.1"
                     /translation="MHPHSRSVLLGIAGASFIAAVLAWHLLGDHPAFFLPVLAFLMAA
                     PVLACLGFLGRPADTARQAWRLLESSPIAVVINRPDGTPLFANTRAHDLAGTDAAGFM
                     ARPVMSWFGDPDFPQAALERLRAGERLHDMEVEFQTVRGDSFWTLTSMESLDLDGRPA
                     IISWSYDITARKTAEQELRKLSLAVEQSPSMLLVTDPDGRIQYANPCYCRTAGLTAAE
                     LVGQPPDLVDLTDAPLRFSAPRHPELKDGGVWLQECRLRTAHPRTQWVRVMVSPLIED
                     ATIRHCIWVLEDLSLHRDAMEALDQAKRLAEEAAASRARFLADMSHEIRTPANAIVGL
                     THLALDSGLPPAQLDHLTRIQSAADTLLAVINDVLDLSKIDAGRLSLEQAPFSLDEMI
                     DRVITLVAPAAHARHLALLLAIAPDVPLRPVGDALRLGQVLTNLLANAVKFTERGFVR
                     LNVVVAARDETSASLHFEVEDTGIGLPPGEVEHLFEAFSQADDSVTRRFGGTGLGLAI
                     SNHLVTLMGGRIEVRSAPGDGSRFGFTVRLGVAPAPAAVELPSALRGAHTLVIDPHTA
                     ARDLLVAQLNALGVPTEGCDTPDAALAWAHDPDRSIRLVLAAAPRGESGPSLTAALHT
                     LAGHGPLPALVIMGPFPAHRDHAGLPAGHLRIPATRSRLRDAVVEALAPTRPEPHELP
                     APPPRALAGRSILVAEDVEINRTITQNFLENAGARVTLANDGDEALRLLQTMGAQAFD
                     AVLMDIQMPVLDGLSATRQLRADSRFAHLPVIAMTAHAMADDRDRCLQAGMTDHVAKP
                     IAPERLIDVLIRHTMRAPQPAPAAGPQPAAVSHPSDLPHIAGIDVPGALPRFNHNVDM
                     YLRILRRFAPHHGDAPARIEAALAAGQRDDAERIAHTLRSSAAGLGADGIADLAAQIE
                     LGLRKGAGIDTPLHGLHEEMTRLLAALDRALPPEETPRPGAPELDAESLDAACRELDA
                     LLRHADGRAPALFLRIRNDLSRRHAPAAVETLADAIARYDYDKALIHLANVMNRAPAP
                     "
     gene            17026..18195
                     /locus_tag="PLH85_08660"
     CDS             17026..18195
                     /locus_tag="PLH85_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HNB64673.1"
                     /translation="MEQTDPTSDQDDHRAIILVVDDAPAAIEPIVDCLHRADFRTRIA
                     TTGERGLALARVEPLPALILLDVIMPGLDGYEVCRRLMADPVTANIPVIFLTARNDVV
                     DEQAGFDAGAVDYITKPISPPTVLSRVRNHLVLKSASDFLRDRNTYLEQEVLRRTREL
                     SLIQDATIVAMASLAETRDNETGNHIRRTQMYLRALARHLQHHPRFAAELSDENIDLM
                     YKSAPLHDIGKVGIPDRILLKPGRLTADEFEIMKTHTTLGRDAISAAESLFGVSARFL
                     RYAKEIAYSHQEKWDGSGYPQGLSGDRIPLSARLMAVADVYDALICRRVYKPPMSHAD
                     AADIMRAGRGTHFDPDILDAFLALLDEFHAIALRFADLEQDHAREIERLRQVFGD"
     gene            18303..21134
                     /locus_tag="PLH85_08665"
     CDS             18303..21134
                     /locus_tag="PLH85_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB64674.1"
                     /translation="MISSTTSRQVVDTVRRYGLALLAFALVLLVSFERYHSTVQQEQA
                     TTHTRFQLMAQEQIARLRERTDELFSHADVLQHYAGQGGSLDPQRFARLLMPMVAHNP
                     EIASAGILRRHADGAPVEVLQVTGATRLTRGADFPNSEAYPDADDRRILLEALRNAPH
                     TATGTATLPVATRDIGGTPADTFLIFAPVREDPDAPPGNQQDAAFLEIRLSRLIDAAF
                     QPAINRDVNQSIGVEVLDATDEARTLLSSPGFRPAELTYADAVPMGNRRWLVIIVPDG
                     PHYSLSPSADAGGQLFAGFSLAALIAFMVYVLQSRTEAIRHRVAEQTVALAEANDALG
                     QYVNALGETNRLLENEVDKHARAEALLRETTSLQRAILDCAEYAIIYTDTAGIIRVLN
                     PAAERLFGYRAEDVIGRDTPLIFYTAEDIAGLTLAGKPSGFAALAVEPLNGAADTPTG
                     AADPREVTLRRRDGETFPANIVVSTVRDASRNVRGYLGIVADITQRRAAESRIRHLAH
                     YDNLTSLPNRTLLNQHLTEVIEQAAVDQRRVAVLFTDLDRFKYVNDTLGHQAGDQLLL
                     SVAQRLRGCVRDGDLVARTGGDEFVVVLSRLEHAGGAEEAAGRILTALARPFELCGQQ
                     FTVTPSIGIALYPNDGLDGEALIKNADAAMYLAKERGRNNFQFFDHGLSERYSERLEL
                     EARLRQALQAREFELYYQPQVDTLSGEIIGMEALIRWPQPDGSLIPPDKFIPIAEECG
                     LIVPIGAWVLEEACRQNQAWIREGIARVPVGVNLSARQFDDKDLLDTIKSALDTAGLA
                     PAYLDLELTESLVMRNPEHTRNLLVECKRLGLQLSVDDFGTGYSSLANLKRFPIDRLK
                     IDRSFIKDIASEPDDATIAQTIVAMAHTLRLEVIAEGVETEAQLALLRRWRCDAYQGY
                     LCSRPINAAAMTQMLRNLRASRLVSLTAL"
     gene            complement(21147..21620)
                     /locus_tag="PLH85_08670"
     CDS             complement(21147..21620)
                     /locus_tag="PLH85_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNB64675.1"
                     /translation="MERGRRRFLTVAAAALTALGGCAVMRLGLDAPEVRVAGIQPVDA
                     TLLEQRFLITLRVLNPNNVEIDVEGLTFAVELNGHAFARGVSNQATVIPRLGEATLEV
                     TATTGLMDVLNQLRQFQKAPEAVPYRLKGRLVTGRFGSIDFDRSGEVSLTRLQPR"
     gene            complement(21623..22501)
                     /locus_tag="PLH85_08675"
     CDS             complement(21623..22501)
                     /locus_tag="PLH85_08675"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB64676.1"
                     /translation="MRVGFVGLGVMGRPMAQHLLAAGHDLAVWARRPASAEALRAAGA
                     AWCESPAALAARSEVVISIVTACADVEALALGADGLLAGFAPGSVHVDMSTIAPATAR
                     QLAGCYAERGVGWLDAPVSGGEVGARDATLAIMAGGEAAVLAQVRPLLDCLGRTVVHI
                     GPAGAGQVAKACNQMVMVNAIQACAEAMCLANAHGVDPERLWTALMGGSAASRVLEVM
                     GGRMARRDFAAGIQSRLHHKDFAILMAAARQGGVPLPVAAQTWQQLNALMAAGGGHDD
                     TASLLKMLEPAGRHGA"
     gene            complement(22504..23376)
                     /locus_tag="PLH85_08680"
     CDS             complement(22504..23376)
                     /locus_tag="PLH85_08680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB64677.1"
                     /translation="MKVGFIGLGIMGRPMALNLLKGGHAVTVWARRADSMAPLLDAGA
                     TGAADAAAVAGDVDVVFSMVADAADVEQVALGPRGVADGARPGLIYVDMSTIAPAAAQ
                     SIAARLAERGVSMLDAPVSGGEVGAINGALTIMVGGDEAAFGTVAPLFACMGKAATLI
                     GASGAGQVAKACNQILTGVGVMAVAEAFNFARKSGVDAARVREALLGGFAYSRILENH
                     GQRMLDRNFKPGFKAWMHQKDMNIVMQEAHRLGLMLPSSAATAQLFNAMVGSGLGEED
                     SIAALKLLEQLSGA"
     assembly_gap    23475..23574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23575..23881)
                     /locus_tag="PLH85_08685"
     CDS             complement(<23575..23881)
                     /locus_tag="PLH85_08685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB64678.1"
                     /translation="MTQRIAAVVVTFNRKALLLECLAGLLAQSRPVDRIFLIDNASTD
                     GTREALEGAGFLSHPLIEYVPMATNTGGAGGFHHGLKVAFEAGYDWFWLMDDDVEPLP
                     "
     gene            complement(23919..25175)
                     /locus_tag="PLH85_08690"
     CDS             complement(23919..25175)
                     /locus_tag="PLH85_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNB64679.1"
                     /translation="MASLRRTLVSLYVIQAANFAVPLLTQPWLTRALGVDGYGRLNFA
                     LAVVAYFVLLADYGFNLSATRQIAVQRDDKAARSRTFWATLGVKALLAFVGGLMLLVL
                     AALSSTLGEISGLLALGYLAVIGSVLTPTWYFQGVERADFAALATLLPKALAIPLIVA
                     GVRGPADLPAAMLLTAAPGLLSGLWCLAELARSRAVAMQRPALADLRAALADGWHVFV
                     STAAISLYTTSNTVLLGWLSGTAAVGHFAAADKLLKAAQAATAPVSQALFPRVSHLMH
                     HDRAAALGTLRRLLKWQGAFTFAVSVGLFVLAPVLVRLLYGEAFEAAVEVVRWMAPLP
                     CLIGLSNVFGIQTLLPLGREKLFSRILLVSGVVNLGLLALLAPVYGAPGAAIAVTITE
                     AGVTALMGWAVRAHFAHRAPVDAARP"
     gene            complement(25185..26354)
                     /gene="glf"
                     /locus_tag="PLH85_08695"
     CDS             complement(25185..26354)
                     /gene="glf"
                     /locus_tag="PLH85_08695"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HNB64680.1"
                     /translation="MSVDAIIVGAGFSGAVLAERFASQLGWKVLVLEQRPHIGGNCYD
                     EPDAHGVLVHRYGPHLFHTDNPRVWDYLSAFTEWRPYEHKVLSSIDGQLVPIPFNLNS
                     IDRLFDADTATCMREALVSRFGLGARVPILRLKEEDEPLLRELADFVYRKAFLNYTTK
                     QWGVKPEEISPEVTGRVPVVVDRDDRYFTDPYQAVPAEGYTKLFEKLLAHPHITVRLD
                     TPFNAVGSFDPDTGAVQFEGAPFGGLLVFTGMVDELFGRDRGALPYRSIRFDFQHHAV
                     PVFQPATTVNYPNEHRYTRITEFKHLTGQQCAGTSIVYEYPQAYDPADPEANVPYYPI
                     FDERSKAAYTAYADRLGGHARVVAVGRLAQYRYFDMDDAVANALQVFDRIAAAQG"
     gene            complement(26401..27855)
                     /gene="wbaP"
                     /locus_tag="PLH85_08700"
     CDS             complement(26401..27855)
                     /gene="wbaP"
                     /locus_tag="PLH85_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HNB64681.1"
                     /translation="MRRPQRPAGEVRGWLVLADALALGVAFGLALLLLLWLRGHRIGP
                     SFAQWWAAEGVNQALAFLLLLVLALVTFAWRGHYHRRQPFWDELLEIIRSLLLAALLN
                     GMVVLLAKWPVSRFLWPVSWGLALVLLPLFRNGMKSALMRAGSWQLPTVIVGVGENAV
                     AAWQALRSEHLLGFEVHAFVAVGPEAPAELVVAPGVTVPVARPDKLLPWLADQGHPHV
                     TVAVSEPELQEIAGQVELLTLHYKDLHIIPPIAGLPLFGLSANHFFSHEVLMLRVRNN
                     LASRPLRWLKRMFDIAGSACGLALLSPLLAYIAVQVKRQGGPGSVFFGHERIGLDGVP
                     FKCWKFRTMTHDSAEVLARLLASDPQAREEWNRDFKLKNDPRITPIGAFLRRSSLDEI
                     PQLWNVLKGEMSLVGPRPVVEAELERYGERVAFYLEARPGLTGLWQVSGRNETTYDER
                     VALDAWYVKNWNLWYDIAILCKTIRTVVSGHGAY"
     gene            complement(27857..>28968)
                     /locus_tag="PLH85_08705"
     CDS             complement(27857..>28968)
                     /locus_tag="PLH85_08705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB64682.1"
                     /translation="CAGARGRVEGIVAHILHVVESFGAGTLSMVSAIANRQAADGHRV
                     SLVYSTREETPANWRALFDPRIATRHVPMTRAIRPATDWASAVALRRILRDWRPDVVH
                     LHSSKAGALGRLLSLVCPGPRWFFSPHGLSFLQKAAGRIKNAVFLTLEKLLARIPVTF
                     IACSPSEAEQIRTHLSRRVVVVPNAVDLAILPQARGGTGVVRIGTVGRVTTARHPELF
                     AEVARRSGRDGVEFVWIGGGEASGEAALRAAGVHITGWVDRARALEELGRLDIYLQTS
                     RWEGLPVAVIEAMGAGLPVIATRVVGNRDLVQDGDTGWLVPEDAAAFVARLGPLVNDP
                     ALRQATGARAQAFVRAHFAFDRMMEALYRAYGLQN"
BASE COUNT         5190 a        10568 c         8838 g         4272 t
ORIGIN      
        1 agcaggaagc gcgcttcccc gaatcccgga ttcttctgca aggcgttctt gagctggatg
       61 gacgccgcat tgcggtcatt cttggccaga tactccttgg ccgacgccac catcgattcc
      121 ggactgccac cacaggccgc cagcaacagc gcacacagca cgctcaccgt ggcgctgcgc
      181 accacgttcc gtccgcttcc tttttgcatc cgcctgattc cttgcatcac gtcgaggtct
      241 ctttcttcag tccgaaccgg ttcatcaagt cgtacagggt cggacggctg atgcccagca
      301 cctcggccgc ccgtgccacg tttccattca cccgagccat cacgcggatc accgcccgcc
      361 gctcggcctc gtcccgcact acacggagat tgagggcttc gagttcacct tcttcgacgg
      421 tgccatcgcc cacgccgata tcgtccgccg agatgcagtt gccgtcggcc atgatgaccg
      481 accgcttgat ggcgttttcc agctcacgga tgttgccggg ccagcggtgg gattcgattg
      541 cctgcaccgc gtcgtcggtc agcgtcatgg tgccgcgacc gttgctcgcc gcaaagcgct
      601 ggacgaaggc gtgggccagc agcaccgcgt caccatcccg atcccgcagc gccggaatat
      661 ccacgacgat ctccgcgagg cggtaataca ggtcttcccg gaagcggccg gtctggatca
      721 gtcccttcac gtcctggtga gtggcgcaga tgatccgcac gtccaccggg atctcctcac
      781 gcccgccaat ccgctcgatc acccgttcct gcaggaagcg cagcagtttg gcctgcagcg
      841 gcatgggcag atcaccgatt tcgtccagga acagggtgcc cttgtttgcc acctcgatct
      901 tgccgagcgt ctgcttgacc gcccctgtga acgcgccctt ctcgtaaccg aagagctcac
      961 tctcgaggag gttttcagga atcgcggcac agttgatggc cacgaagcgc tcgttacggc
     1021 gcggcgacag ttcatgaagc ccgcgggcca gcacttcctt gccggtacca ctctcaccca
     1081 gcaacatgac cgtcacgttg gcggaggcaa ccttttccac cagccggcac acccgcagca
     1141 tgccgggatc gcgcgtgatg acgccggaca gcgcgctgcc ctgctgcatc ttgaggcgcc
     1201 ggttgtcctg ctggaggtcg tacagacgga aggcccgctc cagcgtgagg accagcattt
     1261 cgggttcgaa gggcttggcg aagaagtcat aggcccccat tcccaccgca cggaccgcat
     1321 tttcccggtc gtgctgcccg gtgagcacga tcaccttggt gtcgggcatc agcgcgagga
     1381 tctcgccaag caggcggaac ccctcggtca cgtcatccgg ggcaggaggc agccccaggt
     1441 ccatggtcac cacggccggc tcgtagcgcc ggatctgcgc gatcgcctcg tcccggttac
     1501 tggccaccac cgtatcgtac tggtcgaagg cccaccgcat ctgcttctgc agcgcggggt
     1561 cgtcttcgac aatgagtaag gtcctgcgtt tatcgctcat gtctgcttag ccgccggttc
     1621 gccatccacg ccacccggga gatgcgcaag aggcagacgg atctcgaaac atgatccggc
     1681 accgacctcg ctctcaacgg agagactgcc gccgagctcg tggatatatt gccgggcttc
     1741 gtatgcaccg atgcccatgc cgctggcctt ggtggtctga aagggcttga ataactggtc
     1801 ccggatgaac gctgcggaca ttccggaacc tgtatcggcc acccggatgc tggcgaaatt
     1861 accactccgg gatacctcga ccgacacact accatgctcc gatgtcgcgt ccagtgcatt
     1921 ttgcacgata tgccccacga tacgttccaa gcgttcggaa tgcgccagca cggccagatc
     1981 aggcccgccc gacaccagga cgggcgcagg acggaagagt cgtttggatt gaacgatatg
     2041 ctccaacaat ggtgtgagcg gaacgggctt gcgctggttg atgggcgtct tctccatcaa
     2101 ctgggccatc aggccgcgca tgcgctcttc cacgtgtcgc accgtgtcga gcatgtcggc
     2161 ctggaactcg ggattgtcct tgtgacgctc ggcattgcgc aacatcagag agagctgtgc
     2221 aacgaggttt ttcaggtcat ggactacaaa ggccgacatc cggttgaatg catcgaactt
     2281 gcgtgcctcc agcagcgctt ccgcggcctg cattcgcgca aaatagccgg cggcctgacg
     2341 ccctgccgtc ttgagcagat cgaggacttc ccagttcacg tccaccggcg tgcgcggcgt
     2401 caacaggatc acgaagccga tcaggctgcc agccgagatg aggggcacga tcagccacgc
     2461 atcgtccact tcgccgatcc aggcgggcac gggctgggat tcgccgaaat tcacccctgc
     2521 gcgggcttcc tccagattga ccacccattc cttttcgagc aagaagcgga tgaatgcatc
     2581 gtcatcgccg acctgcatcc cgcaatgggg catgttgagc cgtgcctgct gactgtagcg
     2641 ccccgctgca tccttgagcc agaccccgcc tcccggactt tccaccagat cgcccagcgc
     2701 gttcaccacg gattcaccca gagtgagccc gtctccggcc gccgacagcg cctgcgtgac
     2761 gcccagccac tcggtccggt agtcgtaacg ataagcaaag aaatgtttgg caatcagcac
     2821 cttgaagcgt gatcggacgg ccccggagaa cgccacgacg cccaggaaca gggcgccgcc
     2881 gaacagcagg gccacctgca gggcctgtcc ccactcgcca ccgaagtagc gcatgtaata
     2941 gccggcgccc gcaatcaagg tcaggtaaag ccccgtcccg gccatggccg tcgaatgcag
     3001 cacgacgtgc cgcgagaccg agatgcggaa ggtccattcc gggttgcgcg acgccgatac
     3061 ggcgatgagc gggattgcga gggtgtgggc aaatccgcgc acgctccaga ccgagggatg
     3121 caggcgcgtg aacagcagcg cgtccgagaa gaggtagaca tcgaacgcaa acaggccgcc
     3181 cagcgcgagg gtcaaaggct tgatgcccca atgggagcgc tccggcacgg aacgatagaa
     3241 gtgttccaga agcaccagac cgaacaccga cgaagccagc gatgtggccg cccaggtgcg
     3301 catcggcgtc ccgtaaagcg ggatgccgag cgtgaagcac gccacgcccc ctgccgccag
     3361 ggccacgatc gccagcatcg cgggccgcgg ccaggcgagt gcagcgacgt tcccgtccgc
     3421 ctcgctggcg ttctgcaaca gcatcgccac caggaagccg aaccacgccg cctgacgcag
     3481 gacatcgaac agattgccca gctggaacag ccagatccag cccgtcagcg caaagcccca
     3541 gctcgcagcc gcccaaccac cgcttaatac gacagcggcg accagcagcc agccgcggcg
     3601 cccccgccgc caggcaatcc ccagatacag cgcaaacaac ccatgcagga ccgtcgcgaa
     3661 ggcatagctc cacacggaca cttgggacag gaagagttcc attcaggctg gacaggaaac
     3721 gggcgacccg atgaggggtc gcccggacag gcaggttacg cgcacgacgc tagcgcaggt
     3781 cagtgggcac cgtcgccggt caggaccaca cggacagttt cgaagagcac gacgatgtcg
     3841 aggaacagcg tgtggttctt gacgtagtaa agatcgtact ggagcttgtt gatcgcgtca
     3901 tccaccgacg agccgtactg gtaacgcacc tgggcccagc ctgtgacacc cggcttgacg
     3961 cagtggcgaa cggcgtagaa gggaatgtcc ttggtgagct gatccacgaa gaagggacgt
     4021 tccgggcgag ggccgacgag actcatgtca cccttcagca cgttgaagag ctgcggcagt
     4081 tcgtcgatgc gcgtcttgcg catgatccgg ccaaaacgcg tcacacggtc atcgttggcc
     4141 gacgcccagc ggggcttgcc atcccgctcc gcatcgcggc gcatgctgcg gaacttggtg
     4201 accttgaaca ggcggccatt gagaccgacc cgctcctggc gatagaacac cggcgcgccg
     4261 tcctccatca cgatcagcag catcgccagc agcatgaccg gcgtggcgag ggcgagcagg
     4321 atcagggagg ccacgatgtc gaacacacgc ttgaccaggg tccggcccag cccctgacgg
     4381 aaaccttcgc cgtagatcag ccagctcgcc cgcagggaat cgatgcggac ctgaccgcgg
     4441 acccgttcga agaaactgga gaggtcaagt accttgatgc cggccatctt gcattcgagc
     4501 agttcccgca gcggcagcac gccaccgcga cgctccttga cggcgacgat gatctcattg
     4561 acgcgcagat cgttcgcggc agccagcagg cgtccgtcgt aatggacgat gcgcctcggg
     4621 tcgacccgga cctcatcgcc accactgccc gccgggaaga acccgaccac ctgcagactc
     4681 ggattggccg gatgggtgag cgcgtgctcc accgcgaccg catcggcacc ggttccgatc
     4741 accatcacac gcttcgccaa cacacccact cccatcttcc gcatcgtaaa accgcgtacg
     4801 gccacgacaa agccaagcaa tgcgatcacg tcgagctcca tggcctcgtg cgcgaattcg
     4861 ccccacggca ggacccggaa gattccgtac gccaccggca cgctgagcac gatggagatc
     4921 aacaccctca ccgtggtttc ccgcaaatct gcgccactgc ccgcccgata cagcccgagg
     4981 gcaccgttga gcaccatcat ggtgatggcg aacaccaggg ccgaaggcac gaccacgcgg
     5041 accagactct cgtcgagctg ggtctgcagg aggaacacga ccacgacgga gacaaacagc
     5101 aacgtcacgt cgaacatcag ctgcaggatc gtattcgacg gaacgtagtg attgaaaagt
     5161 ttgagcattc cgaatttcag cccgcttctc agcaggccgc ccatgggatg agggttcgag
     5221 cactgatggt cggggcccgc gacaaccggc aaaaattgcc ggcaattatc gccgatctgg
     5281 gcacccgatc accctccagt catgccgatt gtccgtcacg aacttgccaa ttgcgtgact
     5341 acgaccacgg acgaacaata tcgcctcccg cgtcgcagcg ctgtgaagcc gttcacacaa
     5401 aaatgacaga gcctttccgg caagaccaaa ccgctacgac acaaacggag cgattctaat
     5461 gccctgggca tagaagaaac gttaatctct atgtcttgca ggcgtttagg ggcacaacca
     5521 ccctgcctcg gcgcaacaaa gtgtgtccgc ccctgcccga ttcaggcgtg ttcgcgtcgg
     5581 gaaccgccta caactcgatc tgggtgccga actcgacgac acggttgacc ggaatgcgga
     5641 agaagtccgc cgcgctgtcg gcgttgcgga acatgatgtt aaaaagatgc tcgcgccaca
     5701 ggggcatcgc cgatcccttg cgcgggatca gcgtttcgcg ccccaggaag aaggacgact
     5761 gcatcagttc gtagggcagg cccttttcgg cacacagcgc cagcgcggcg ggaatatccg
     5821 gatcgtcctt gaaaccatag cggacgaaga cccggaagaa cccgaaatcc agttcctcga
     5881 ggcggacgcg ctcttccagg ggaacgtagg gcacatcggc tgtcaccaca tggaccagca
     5941 ccacgctggc gtgcagcacc ttgttgtgca gcatgttgtg gagcaacgcc cgcggcaccc
     6001 cgtccggatt ggtggtcatg aagatcccca gcccgggcac ccggtgtggc ggatcgtgaa
     6061 acatggactg gatgaacaga tcgagcggca tcgtatccac ccgcatccgc tccgtgagca
     6121 gcgcccgacc agtcttccag gtcatcagca gcgtgtgaac gcccagcccg agggccagcg
     6181 gaaaccaccc gccgtcgagg atcttcacac tgttggcggc gaagaacgag aaatccacgg
     6241 agaagaacaa ggcgacgaag gcaaccgctg cactccagcg ccagtgccac agggcacgca
     6301 ccacgacaaa cgcaaggacg gtgtcgatca tcatcgtcag ggtcaccgca atgccgtaag
     6361 cggaagccag cgcgctcgac gacttgaagc cgatcaccag cgcgatcacg gcccccagga
     6421 gcatccagtt tacgttcggc acatagatct ggcccgcttc ccggtccgag gtatggacga
     6481 tgtggatgcg cggcgagtag cccagctgga cggcctgttt ggtcaacgaa aacgccccgg
     6541 agatcaccgc ctgggaggcg atgatggtcg ccaccgtcga gagcccgacc atggggtaga
     6601 gcagcggatc cggcaccagc aggtagaacg gattggcgac cgccgacgga tcgtcgatga
     6661 tgagggcgcc ctgaccgaga tagttcagat agaggcacgg aaagacaaag ccgaaccatg
     6721 ccagcttgat cggccgccga ccgaaatgcc ccatgtccgc gtagagcgcc tcgccgcccg
     6781 tgatggcgag caccaccgcg ccgagggcca gaaagccatg aacacggttc tccacaaaga
     6841 accggacagc gtgcaggggg ttcagcgctc ccagcacgtc cggatggcgc acgacgttga
     6901 aggcgccaag gactccgagg gttgcaaacc agaacagcat gaccggcccg aatacggcac
     6961 cgacctgcgc cgtacccttg cgctggatga cgaacagccc gatcagcacg accagggaga
     7021 tcggaatcac gaagggcctg aatgcggggg tcgccacctc caggccctcc accgcggaca
     7081 ggacggaaat ggccggggtg atcacgccat caccgtagaa cagggccgca ccgaagagtc
     7141 cgagtgccgc catcagggat gccgcacgcg gcgagtcggt gctgcgcaag gccagcgaag
     7201 tcagcgccat gatgccgccc tcgccccggt tgtcggcacg catgatgtac accacgtact
     7261 tgagcgacac cacgaaggtc agagcccaga acaccaggga caggatgccc agcacgtttt
     7321 ccggtgtggc gggcacggca tgcgggccgt tgaacacttc cttcatcgtg tacagcggac
     7381 tggtgccgat atccccgaac accacgccca tcgctgccag cgccaccggc aataccttcg
     7441 tacccgaatg cccgtgctga tcttccgcga caaccgccga cattccactc tccccgactc
     7501 atagtcattg ccgcccggac cacggccggc ggcgggcgca ttataggtga agtatgctgc
     7561 gctgccgcag atcgctgaac ttttcgagcg gggcccggtc acccgtcctt catgccgagc
     7621 cgttccaggc gatagcgcag ggacctgaag gtcacgccaa gcgcccgggc ggcggccgtg
     7681 cgattgccgc ccgcgcgctc cagcgcctcc acgatggcct gacgctctac gtgatccagg
     7741 tagtcctgca aaggcgcgcc accgtcctgc accacgatcg acggcgattc gcccgccagc
     7801 tcctcggcag gctccagcaa cagatcggcc gccgtgatca cgttgccatt ggacagcgcc
     7861 agcgcccgct ccagaatgtt ttcgagttcc cgcacattac ccggaaaggc gtacccgacg
     7921 agggcctgca gggcatccgg gccaaggcgc gggacatcca tcccggaatt ttcggcgaga
     7981 cgccccagca gggtggtcgc cagctccggg atgtcctcac tgcgttcccg cagcgccggc
     8041 atgcgcagtt cgatgacgtt gaggcgatag tagagatcct gccggaagct ggaggcttcg
     8101 accatcgatt tgagattgcg atgggtggcg ctgatgaggc gcacatccac gccgacctcg
     8161 gtggtgtcac ccaggcgcct gaagcgcttc tcctggatcg cccgaagcag cttgacctgc
     8221 atgggcaacg gcaggtcgcc cacttcgtca aggaacagcg tgcccccgtg agccgcctgg
     8281 aagaagccct cccgatccgc atcggcgccc gtgaaggccc ctcgcttcac gccgaagaat
     8341 tcgctttcca tgaggctctc ggggatcgcc ccgcagttga ccgccacaaa gggcgcaccg
     8401 ctgcgcggac caagctcgtg gatcatgcgc gcggcacgct ccttgccgct gcccgattcg
     8461 ccggaaatca gcaccggcgc ctgacttcgc gccagcttct cgatcagcgc acgcacctgc
     8521 accatggccg ctgaatgacc tcccagactg gacgtatccc gggtgccctc cacgccatcc
     8581 gcgggcgaca cggagaacac ggacttcacc agggcccgca gctgatccac cgacacaggt
     8641 ttgctgaggt agtcgaaggc cccggccttc agcgcggtca cggcattgtc catgctgccg
     8701 aaggcggtga tcaccgccac cggcaggtcg ccgaccttgt ccgcaatgaa acgcaccaga
     8761 tccagcccat ccccgtcggg cagacgcata tcggtcaggc acagacgata gcgccgttca
     8821 gtgagcaggc gctgcgcgtc ggccaccgtg cccgcaccgt cgcaggccag gcccatgcgc
     8881 atcagggtca gttcgagaag ttcgcggatg tcgtcctcgt cgtccacgat gaggacgtcc
     8941 ggcccgctgg cgcgggcacg tcggtcgatg gagttcatga tctgctgcgt gccgtgagtc
     9001 gaaaatgggc accgggcagg ccgcccgcct cggcctggcc cagttcgagc acagcaccgt
     9061 tggcatcggc cagttcccgg gcaatataga gcccgagacc ggttcccttg gaatgtgtgg
     9121 tgaagaaggg ttcaaagacc tggttgcgaa gcgcctcggc aatccccggg ccgtcgtcgg
     9181 tcacgtccac cgcgatccgg ccgtcgtccc gcagacccgc ggcgatgcgg atggagcccg
     9241 gcgcaccgct gccatgccgc tgagcgttgg cgagcaggtt ccacaggatc tgacgcaggt
     9301 gcgtacgatc catcagcagg gtcaggtcgg atgcaatcgc cacatcgacc agcgcgcgcc
     9361 gcaccggatc ggtgagggcg aattcgtcga gaaattcatc cacgaactgt ttcaggggca
     9421 gcacttccgg cagggcctcg tcgcgccgcc ccagcgccag cacatcgccg accagccggt
     9481 cgatgcgctg cgcgttggtg ccgatgatgc ggatcaggcg ggcctgcatg tcgccccgct
     9541 tttcgtcgga cagcagctcc gcagcgtggg agaccgccgc cagcggattg cggatctcgt
     9601 gggcgatact tgccgtcagc cttccgaggg cggccagctt gacctgctgg atctggtcat
     9661 ggatgaggtc gaaatcctcg aggtagacca gggtcgcgtc cgaatcaccg gccctcagca
     9721 tgcgcgccag caggagcttg ccgctccctt ccgcgcgaaa acgcagggcc gaatgggcac
     9781 ccggctcgcc gtcctcaccc gcccccagcg gtccggccag tgctggcgca cagctggcca
     9841 gcgcaccgcc ctcgcgcagc gttccgccca gcagggcatt ggcctgagga ttgaagtgac
     9901 gcaccacgcc gccgggctcc accaccagca caccgtcctg catgtcctgg atgatctgct
     9961 ggttgaggcg caactgcgcg gccagatccg caccccgttg acgcgccagg gtctcgttgg
    10021 cgatcgctcg caaggccagc aagcgcgcca cctgggcaat cgtgaagaaa ccgatgcagg
    10081 tcacgccgac ggtgaagaaa tccaccacat cgcgcccgga gaggaagcgc agcagatttt
    10141 cgaacagcac cgccactgtg gcatacgctg cgtagaacag caccatccga ccctgcccga
    10201 ccagcccaga accggccagg acgatcatga tcaggatggg catgccgctg cggtagccac
    10261 cgctggccca catgatcacc gtgagcaggg tgacgtccac cagcacctgc accgtcacca
    10321 gacgctccat gcccaggtga cgggccgcat cggggaatcc gaggatcaga accgccgtga
    10381 ggtaggcaaa cgcggcgccg aagaacaggg ttgccgaatc gccccccagg ctgaggccgg
    10441 gcccggccac cagaaaaacg ccggccagca ccaggcggaa gagattgaga tagcgcaggg
    10501 gctcccaccg cccacggaac tccgtggccc cggattccag ccccgggtac gccactgtct
    10561 tcacacccgc ggaccaagcc ggcggtgggc gtccgagcag aagaaatacc cgccgacgtg
    10621 cagaccttcg ctttccggca catgcacccc gcaatgggcg caggccacca tcgcctcggc
    10681 ctccgtcggc tgccggggcg cttcggagcg cgcctcccgc cccgccccgg gtgtgtcgcc
    10741 ggactttcct tgcaaggcac gccgcacgta atacaggccg atcaggaaca acacgaagac
    10801 aagcagattt ctcaccggaa cgcctccaga ctgacgggcc agtttagcag aagcgtgcca
    10861 gccgctcgca ggcctccgcc agggtcgcct cctgcttggc gaagcagaag cggatcaccc
    10921 cttcatcccg cccgtcaccg aagaaggcgg acaggggaat cgccgccacg cccacctcgc
    10981 gggtgagcca ctcgacgaac gccaggtccg gcaggtcgga cacggccgcg taacggaccg
    11041 tctggaaata ggtcccggcg cagggcagca attcgaagtg tgtcgccgcc agccccgccc
    11101 ggaaggcgtc ccgcttggcc tggtagaacg cagccagcgc gagatggcgg gacggctctg
    11161 ccatgtaatc cgccagggcc agctggaccg gggcattcac cgtgaacaca ttgaactgat
    11221 gcaccttgcg gaactcgtcc atcagggcct gcggcgcgag cacgtagccg accttccagc
    11281 cggtcacgtg gtaggtcttg ccgaagctcg agacgatcac gctgcgcgcc gccagctccg
    11341 gatcccgcgc ggcgctttcg tggcgggcac cgtcgaagac gatgtgctca tacacttcgt
    11401 ctgccaccac cacgatgccc gtgccgcggg tcaccgccgc cagctctatc atgtccgcgg
    11461 ccgaccacac cgcgcccgtc ggattgtggg gcgtgttgat catgatcatc cgcgtccgcg
    11521 gcgtgatcag cgcccgcagc gcggcccagt ccggccgata ttccggcgcg ccaaggcgga
    11581 cacgacggac cactccgccc tgcagttcga tggccgggac gtaagagtca tagaccggct
    11641 cgaagacgat cacctcatcg ccgggatgca ccagcgccgc caccgcggtg aacaaggcct
    11701 gggtggcgcc cgccgtgacc gtgatctccg ctcccggatc gtagtgcgca ccatagagcg
    11761 catgcacctt ggtggcgatg gcgtcgcgca gcgcgggcat gcccgccatc ggcgcgtact
    11821 ggttgaaccc cgcccgcatc cagtgggcca cgcgttcgaa gagcacgtcc tcggcgctga
    11881 aatcgggaaa gccctgggac aggttgatcg ctccgcaggt ctgcgccagc tggctcatgc
    11941 gggtgaagat ggtggtgccg acggtcggca atcgcgagac cagcggcgcg gaaaagaccg
    12001 gcatcagtga caaggcccga aaaggtgtcg gtccatttta acgccatgcc gcgtgtccgt
    12061 ccgaggtgcc cggcggctac aattcctgca tgcagacccc atcgaacgcc cccgtgaccg
    12121 acacctgccc caccctgcgc cgggagggtg acagcgtcct gatcctcgac cagacccgcc
    12181 tgcctcacga ggaggccttc gtccgcctcg ccacactggc ggatgcagcc ttggcgatcc
    12241 gcagcatgca ggtccgcgga gcaccgctga tcggcgccac ggccgcctat ggcgtcgccc
    12301 tggcgctggc atccgacgcc tccgacgcag ccctcgccga tgccgacgcc tgcctgcgca
    12361 ccacccgccc cacggccgtc aacctgcact gggccctcga ccgcatgggc cggaagctgg
    12421 cgtccgtggc gccggccgac cgccgcgagg ccgcctgggg cgaggccgac gccatccggc
    12481 gcgaggacgc cgacgccaac gcggccatcg gacaacacgg ccttgccctg ctgaacgccc
    12541 tggccgccga gccaggacga ccggtccacg tcctgaccca ctgcaacgcc ggctggctcg
    12601 ccacctgcgg ccatggcaca gccctcgcac cggtctatgc ggcccacgcc gcgggcctgc
    12661 cggtccatgt gtgggtcagc gagacccgtc cccgcaatca gggcctgctc actgcctggg
    12721 agctgcgccg cgacggcatt ccccacaccc tcatcgccga caacgcggcc gcctggctga
    12781 tggccgaagg acgcgtcgat tgcgtcatcg tcggcgccga ccgcatcgcc gccaatggcg
    12841 acaccgccaa caaggtgggg acctgcctca aggccctcgc cgcccgcgcc cacgctctgc
    12901 ccttctatgt ggcggcccct ctgagcaccg tggaccgcag ttgccccaac ggccgtgcca
    12961 tccccatcga agcacgggac ggggacgaga tccggcggat cggtggactg gacgacaacg
    13021 gacggcccgc cttcgtgcac atcgccccac cggacagccc ggtctgcaac ccggccttcg
    13081 acgtcacccc ggcggccctg atcgacggca tcgtcacccc ggtcggcgtg tgtgccgccc
    13141 acgacatcgg ccggctcgcc ggcagcctga gtggagactc cccatgatcg agaatgtcct
    13201 gcgcgaagcc ctcctcgcca ccgcccagtc catgggcagc ctcggcctca acaccggcac
    13261 ggccggcaat gccagtgtcc gctgtcacga caacctgctc atcacgccta ccggctgcga
    13321 ttacgccagc tgcacgccgt cggacatggt gctgatggcc atggacggaa cctggcaggg
    13381 ccactgtgcg ccgtccagcg agtggcgcat ccatcgggac atcctcgccg cccgcccgga
    13441 agccggcgcc gtgctccatg cccattcccc cttcgccacc gccctcgcct gccaccgccg
    13501 cggcattccc gccttccatt acatgatcgc ccgcttcggc gggaccgaca tccgctgtgc
    13561 gccctacgcc accttcggca cccaggccct gtcggatggt gcgctcgccg cgctggaggg
    13621 tcgcagcgcc tgtctcctcg ccaaccacgg catgctcgtc atcggcagcg acctgcgccg
    13681 cgccctggcc ctcgcagtcg aattcgaatc cctctgcgcc cagttctggc gcgcatgcca
    13741 gatcggcacg ccgaccctgc tgtcggacgc cgaaatggcc gacgtcctcg ccaaattccg
    13801 ccactacgga cagcccgccc aggcggccct gctcgccgac gcgctgcccg ccgcggggcc
    13861 cgccgccagc ggactctccg acggctgaac gttcgcgccc atgcatcccc acagccgcag
    13921 cgtcctgctc ggcatcgccg gcgcaagctt tattgccgcg gtgctggcct ggcacctcct
    13981 cggcgaccac cccgcattct tcctgccggt actggccttc ctgatggcgg ccccggtcct
    14041 cgcctgcctg gggttcctcg gcagaccggc cgacaccgcg cggcaggcgt ggcgcctgct
    14101 ggagtccagt ccgatcgccg tggtcatcaa tcgccccgac ggcaccccgc tgttcgccaa
    14161 cacgcgcgcc cacgatctgg ccggaaccga cgccgccggc ttcatggccc gcccggtgat
    14221 gagctggttc ggcgatccgg attttcccca ggccgccctc gagcggctgc gcgccggcga
    14281 acgccttcac gacatggagg tggagttcca gaccgtccgc ggcgattctt tctggaccct
    14341 gacctccatg gaatccctgg acctggacgg caggccggcc atcatcagct ggagctacga
    14401 catcaccgcc cgcaagaccg ccgagcagga actgcgcaag ctgtccctcg ccgttgagca
    14461 gagtccgtcg atgctgctcg tcaccgatcc ggatggccgc atccagtacg ccaatccttg
    14521 ctactgccgg accgccggcc tgactgcggc cgaactggtt ggccagcccc cggacctcgt
    14581 cgatctgacg gacgcgcccc tgcgtttctc cgccccccgt caccccgaac tcaaggacgg
    14641 cggcgtatgg ctgcaggaat gccgcctgcg cacggcacac ccacgcacgc aatgggtgcg
    14701 cgtcatggtg agccccctca tcgaggacgc cacgatccgc cattgcatct gggtcctgga
    14761 agacctcagc ctgcatcggg acgccatgga ggccctcgat caggccaagc gcctcgcgga
    14821 ggaggccgcc gcgtcgcggg cacgcttcct cgccgacatg agccacgaga tccgcacccc
    14881 cgccaacgcc atcgtcggac tcacccacct ggccctggac agcgggttgc cgccggccca
    14941 actggatcac ctcacccgca tccagtccgc cgcagacacc ctgctggcgg tcatcaatga
    15001 cgtgctcgac ctctccaaga tcgacgccgg ccgcctctcg ctggagcagg cgcccttcag
    15061 tctcgacgag atgatcgacc gggtcatcac cctggtcgca cctgcggccc acgcccggca
    15121 cctcgccctg ctgctggcca tcgcgccgga cgtcccgctc cgccccgtgg gcgacgcact
    15181 gcgtctcggg caggtgctca ccaacctcct cgcaaatgcc gtcaagttca ccgaaagagg
    15241 cttcgtccgg ctgaatgtcg tggtggcagc gcgcgatgaa acgtccgcaa gcctgcactt
    15301 cgaggtggag gacaccggaa tcgggctgcc gcccggtgaa gtcgagcacc tcttcgaggc
    15361 cttctcccag gccgacgatt cggtgacccg gcggttcggc ggcacgggtc tcggactcgc
    15421 catctcgaat cacctggtga cgctcatggg gggacgcatc gaggtccgca gcgcccccgg
    15481 tgacggcagc cgcttcgggt tcacagtgcg tctgggcgtc gcccccgccc cggcggcggt
    15541 cgaattgccg agcgcgctgc ggggcgcaca caccctggtc atcgaccccc acacagcggc
    15601 ccgcgatctg ctggtggccc agttgaatgc gctcggcgtg cccaccgagg gctgcgacac
    15661 cccggacgcc gccctcgcgt gggcccacga tcccgaccgc agcatccgcc tggtcctggc
    15721 cgcggccccg cggggcgaga gcggcccttc cctgaccgcc gccctgcaca cccttgcagg
    15781 ccacggtccg ctgcccgcac tggtgatcat ggggccgttt cctgcgcatc gcgaccacgc
    15841 cggcctgcca gccggccacc tgcgaattcc cgcaacccgc agccgcctgc gcgacgccgt
    15901 cgtcgaggcc ctcgcgccga ccagaccaga gccccatgag ctacccgccc ccccgccgcg
    15961 cgccctcgcc ggacgctcca tcctggtggc cgaggacgtg gagatcaatc gcacgatcac
    16021 ccagaacttc ctcgagaacg cgggcgcccg cgtcaccctc gccaatgacg gcgacgaggc
    16081 cctgcggctg ctccagacca tgggcgccca ggccttcgac gccgtcctca tggacatcca
    16141 gatgcccgtt ctggacggac tgagcgccac gcggcaactg cgcgccgaca gccgcttcgc
    16201 ccacctcccg gtgatcgcca tgacggccca tgccatggcg gacgaccggg accgctgcct
    16261 gcaggccggc atgaccgacc acgtggccaa acccatcgcg ccagaacgcc tcatcgacgt
    16321 tctgatccgg cacaccatgc gcgcccccca gccggctccg gccgcggggc cgcaacccgc
    16381 cgccgtctca cacccctcgg acctgccaca catcgccggc atcgacgtgc ctggcgccct
    16441 gccgcgcttc aaccacaacg tcgatatgta cctgcgcatc ctgcgccgct tcgcgcccca
    16501 ccacggcgac gcccccgccc gcatcgaagc cgccctcgcc gccggccagc gtgacgacgc
    16561 cgagcgcatc gcccacaccc tgcgcagcag tgcggccggt ctgggcgcag acggcatcgc
    16621 cgatctggcg gcacagatcg aactcgggct gcgcaagggc gccggcatcg acacaccgct
    16681 gcacggcctg catgaggaga tgacccgcct cctggccgct ctggaccggg cactgccgcc
    16741 ggaagaaacc ccgcggcccg gtgcgcccga actcgacgcc gaatccctgg acgccgcgtg
    16801 tcgcgagctc gacgccctcc tgcggcatgc cgacggacgc gcgcccgcgc tgttcctgcg
    16861 catccgtaac gatctttcac gtcgccacgc gcctgccgcc gtggaaactc ttgcagacgc
    16921 catcgcccgt tacgattatg ataaagcgtt gatacacctc gctaatgtca tgaaccgcgc
    16981 cccggcgccg tgaaccgcgc cgcgccccgg agatcacact tccccgtgga acagaccgac
    17041 cccacatccg accaggacga tcaccgcgcc atcatcctgg tagtggacga cgcgcccgcc
    17101 gccatcgagc ccattgtcga ctgcctgcat cgggccgact tccggacccg catcgccacc
    17161 acgggcgagc ggggcctcgc cctcgcgcgg gtcgagcccc tccccgcgct gatcctcctc
    17221 gacgtcatca tgcccgggct cgacggctat gaggtctgcc gtcgcctgat ggccgatccc
    17281 gtcacggcca acatcccggt catcttcctg accgcgcgca acgacgtggt ggacgagcag
    17341 gccggattcg acgccggtgc agtggactac atcaccaagc cgatcagccc gcccacggtc
    17401 ctcagccgcg tccgcaacca cctcgtactc aaatcggcca gcgattttct gcgcgaccgc
    17461 aacacctatc tggagcagga agtcctgcgc cgcacgcgtg aactgagcct catccaggac
    17521 gccaccatcg tcgccatggc ctccctcgcc gagacccgcg acaacgagac cggtaatcac
    17581 atccgccgca cccagatgta cctgcgcgcc ctggcgcggc acctgcagca ccacccccgc
    17641 ttcgccgccg agctctccga cgagaacatc gacctgatgt acaagtccgc ccccctgcac
    17701 gacatcggca aggtgggcat tcccgaccgc atcctgctca aacccggccg cctcacggcg
    17761 gacgagttcg agatcatgaa gacccacacc accctgggtc gggatgcgat cagcgccgcc
    17821 gaatccctct tcggcgtctc ggcgcgcttt ctgcgctacg ccaaggaaat cgcctactcc
    17881 caccaggaaa aatgggacgg cagcggctat ccgcagggac tctccggtga ccggattccc
    17941 ctttccgcgc gcctcatggc ggtagccgac gtctacgacg cgctcatctg ccggcgtgtg
    18001 tacaaaccgc ccatgagcca cgccgacgcc gcggacatca tgcgtgccgg acgcgggacc
    18061 cacttcgacc ccgacatcct cgacgccttc ctcgccctgc tggacgaatt ccacgccatc
    18121 gccctgcgtt ttgccgacct ggagcaggat cacgcccgcg agatcgaacg cctccgccag
    18181 gtgttcggcg actgagctgc cgcctgtccg ccgcggagcc gattcaagtc caccgcccca
    18241 gggtcgttat ctgaggataa cggaggcgcc cgcccgaggc accccattcc ccaggagtcc
    18301 ggatgatttc cagcacgacc tcccgtcagg tcgtcgatac cgtacggcgc tacgggctgg
    18361 cgctgctcgc gtttgccctg gtgctgctcg tgagcttcga gcgttaccac tcgaccgtcc
    18421 agcaagagca ggcgaccacc cacacgcgct tccagctcat ggcccaggag cagatcgccc
    18481 gtctgcgcga gcggaccgac gagctgtttt cccacgcgga cgtcctccag cactatgccg
    18541 gtcaaggcgg atccctggac ccccagcgtt ttgcacgact gctgatgccc atggttgcac
    18601 acaatccgga gattgcgtcg gccggcatcc tgcgccgcca tgccgacggc gcccccgtgg
    18661 aggtcctcca ggtcaccggc gccacgcgcc tcacacgggg cgccgacttc ccaaacagcg
    18721 aagcctaccc cgacgccgac gaccgccgca tcctcctcga ggcactccgc aatgcgcccc
    18781 acacggctac cgggacggca acgcttccag tggccacccg cgacatcggc ggcacaccgg
    18841 cggatacctt cctgatcttc gcaccggtcc gcgaagaccc ggacgccccc ccgggcaacc
    18901 aacaggatgc cgcctttctg gagatccgtc tgagccgcct cattgacgcg gccttccagc
    18961 ccgccatcaa ccgggacgtc aatcagagca tcggcgtgga ggtccttgat gctaccgacg
    19021 aggcccgcac cctgctttcg tcaccgggct ttcgcccggc agagctcacc tacgccgacg
    19081 ccgtacccat gggcaatcgc cgctggctgg tgatcatcgt gcccgacggt ccccactact
    19141 cgttgagccc cagtgcggac gccggcgggc agctcttcgc cggcttcagt ctggcggccc
    19201 tgatcgcctt catggtgtac gtgctccaga gccgcaccga agccatccgg caccgcgtgg
    19261 ccgaacagac ggttgccctc gccgaggcca atgatgccct cggacaatac gtcaacgccc
    19321 tgggcgaaac caaccgcctc ctcgaaaacg aggtagacaa gcatgcccgc gcggaagccc
    19381 tcttgcggga gacgacctcc ctgcaacggg ccatcctcga ctgcgccgag tacgccatca
    19441 tctacaccga caccgccggc atcatccggg tcctcaatcc cgccgccgaa cgcctcttcg
    19501 gctaccgggc cgaggacgtc atcgggcgcg acacaccgct gatcttctac acggcggaag
    19561 acatcgcagg tctgaccctc gccggcaagc cctcaggctt cgcggccctc gccgtcgagc
    19621 ccctgaacgg cgccgccgac acgccaaccg gcgccgccga cccccgcgaa gtcaccctgc
    19681 gccggcgcga cggggagacc tttcccgcca acatcgtggt ctccacggta cgcgatgcca
    19741 gccgcaacgt ccgcggctac ctgggcatcg tcgccgacat cacccaacgg cgcgcggccg
    19801 aaagccgcat ccgccatctg gcccattacg acaacctcac ctccctgccc aatcgcaccc
    19861 tgctcaacca gcacctcacg gaggtgatcg aacaggccgc cgtggatcag cgccgggtag
    19921 ccgtactgtt taccgacctg gaccgcttca agtacgtgaa cgataccctc ggtcatcagg
    19981 ccggcgacca gctcctgctc tccgtcgccc agcgcctgcg cggctgcgtg cgcgacggcg
    20041 atctggtggc gcgcaccggg ggggacgagt tcgtcgtcgt gctgagccgg ctggaacatg
    20101 ccgggggcgc cgaggaggcc gccgggcgca tcctcacggc cctcgcccgt cccttcgagc
    20161 tgtgtggcca gcaattcacc gtcacgccca gtatcggcat cgccctgtac cccaacgacg
    20221 gcctggacgg cgaagccctc atcaagaacg cggacgccgc catgtacctg gccaaggaac
    20281 gcgggcgcaa caacttccag ttcttcgacc acgggctgtc ggaacgctat tccgaacgcc
    20341 tcgaactgga ggcacgcctg cgccaggcgc tgcaggcacg ggaattcgag ctctactatc
    20401 agccgcaggt ggacaccctg tcgggcgaga tcatcggcat ggaggccctg atccgctggc
    20461 ctcagcccga cggcagcctc atcccgccgg acaagttcat ccccatcgcc gaagagtgcg
    20521 gcctcatcgt gcccatcggc gcctgggtgc tggaggaagc ctgccgccag aatcaggcgt
    20581 ggatccggga gggaatcgcc cgcgtaccag tgggcgtcaa cctctcggcg cggcagttcg
    20641 acgacaagga cctgctcgac accatcaagt ctgcactcga caccgccggg ctcgcccccg
    20701 cctacctgga tctggaactc accgagagcc tggtcatgcg caatccggaa cacacccgca
    20761 acctgctggt ggaatgcaag cggctgggcc tgcaactgtc ggtggatgac ttcggtaccg
    20821 gctattccag tctggccaac ctgaagcgct tcccgatcga ccgtctcaag atcgaccgca
    20881 gcttcatcaa ggacatcgcg tccgaacccg acgacgccac catcgcccag accatcgtgg
    20941 ccatggccca caccctgcgc ctggaggtga tcgccgaagg cgtcgaaacc gaagcccagc
    21001 tcgccctgtt gcgccgctgg cgctgcgatg cttaccaggg ctacctgtgc agccgaccca
    21061 tcaacgccgc ggctatgacg cagatgctgc gcaacctgcg cgcctcacgc ctggtgtccc
    21121 tcaccgccct ctgaacacga ccgcgctcag cggggctgca gacgggtcag ggacacctcg
    21181 ccgctgcggt caaagtcgat ggaaccgaaa cgcccggtca ccagccggcc tttcaggcgg
    21241 tagggcaccg cctcgggcgc cttctggaac tggcgcaatt ggttgaggac gtccatcagc
    21301 ccggtggtgg ccgtcacctc cagcgtcgcc tcgcccaggc gcggaatcac ggtcgcctgg
    21361 ttgctcacgc cacgggcaaa ggcatgaccg ttcagctcca ccgcgaaagt cagcccttcc
    21421 acgtcgatct ccacgttgtt ggggttgagc acccgcaggg tgatcaggaa gcgctgctcc
    21481 agcagcgtgg catccacggg ctgaatgccg gccacgcgga cctctggcgc gtccagcccg
    21541 aggcgcatca ccgcgcaccc gcccaaggcc gtcagcgccg ccgccgcgac cgtcaaaaat
    21601 cgccgtctgc cccgttccat ctttacgctc cgtgtctgcc cgccggctcc agcatcttca
    21661 gcaggctcgc cgtatcgtca tgacctccac ccgctgccat cagcgcattg agctgctgcc
    21721 aggtctgggc cgccaccggc aagggtacgc ccccttgccg cgccgccgcc atcaggatcg
    21781 caaaatcctt gtggtgcagg cgcgactgga tgcctgccgc aaagtcccgc cgcgccatgc
    21841 gcccgcccat cacctccagc acccgcgacg ccgccgaacc gcccatcagc gccgtccaca
    21901 gccgctccgg gtcgaccccg tgggcgttgg ccaggcacat cgcctccgca caggcctgga
    21961 tcgcgttgac catcaccatc tggttgcagg ccttggcgac ctgcccagcc ccggcaggac
    22021 cgatatggac caccgtacgc cccagacagt ccagcagcgg ccgtacctgc gccagtacgg
    22081 cggcctcgcc gcccgccatg atggccaggg tcgcatcccg cgcgcccacc tcacccccgg
    22141 acaccggcgc atccagccag cccacgccgc gctcggcgta gcacccagcc aactgccgcg
    22201 cggtggcggg cgcgatggta ctcatgtcca cgtgcacgct gccgggggca aagccggcca
    22261 gcagcccgtc cgcccccagg gccagcgcct ccacatccgc gcaggcggtg acgatggaga
    22321 tcaccacctc gctgcgcgcc gccagggcgg ccggtgattc acaccaggcc gctccggccg
    22381 ccctcaaggc ctcggccgag gccgggcgtc gcgcccacac ggccagatcg tgaccggccg
    22441 ccagcagatg ctgggccatc ggccgcccca tcacccccag tccgacgaat ccgacccgca
    22501 tgctcaggct ccggacagct gttccagcag cttgagggcc gcgatggagt cctcctcacc
    22561 cagcccgctg cccaccatcg cgttgaagag ctgggccgtc gccgcgctgc tgggcagcat
    22621 caggcccagg cggtgggctt cctgcatcac gatgttcatg tccttctggt gcatccaggc
    22681 cttgaaacca ggcttgaagt tgcggtccag catgcgctgg ccgtggttct ccaggatgcg
    22741 gctgtaggca aagccgccca gcagcgcctc ccgcacgcgg gccgcgtcca cgccgctctt
    22801 gcgggcgaag ttgaaggctt ccgccaccgc catcacgccc acccccgtaa ggatctggtt
    22861 gcaggccttg gccacctgcc cggcccccga ggcgccgatc agcgtggcgg ctttgcccat
    22921 gcacgcgaag agcggcgcga cggtgccgaa ggccgcctcg tcgccgccca ccatgatggt
    22981 cagcgcaccg ttgatggcgc ccacctcgcc gccggagacg ggcgcgtcga gcatgctcac
    23041 gccccgctcc gccagtcgcg ctgcaatcga ctgggccgcc gccggggcga tggtgctcat
    23101 gtccacatag atcaggccgg gccgggcgcc gtccgccaca ccgcgggggc ccagcgccac
    23161 ctgctccaca tcggcggcgt ccgccaccat gctgaacacc acatccacgt cacccgccac
    23221 cgccgccgcg tcggccgcgc cggtggcgcc ggcgtccagg agcggcgcca tggaatcggc
    23281 ccgccgcgcc cacacggtga cggcatgtcc gcccttgagg agattcagcg ccatgggacg
    23341 ccccatgatg cccagaccga tgaatccgac tttcatgttc gacctttccg attaaaacgt
    23401 gacgcaccgg gccttcaggc cgcggcgccg aaatacagcc tgagtccgtc ccgcacgccc
    23461 cgcagcaccg tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgggca
    23581 gcggctccac gtcgtcgtcc atcagccaga accagtcgta gcccgcctcg aaggccacct
    23641 tcagaccgtg atggaagccg ccggcgccgc cggtattggt cgccatcggc acgtattcga
    23701 tgaggggatg ggacagaaag ccggcgccct cgagggcttc ccgcgtgccg tcggtgctgg
    23761 cgttgtcgat caggaaaatg cggtccacgg gccggctctg ggccaacagg ccggccaggc
    23821 attccagcag gagcgcctta cggttgaaag tcaccaccac ggcggcgatg cgttgggtca
    23881 tggtcggaag tccggtttca gttcaggtcg gggacaattc aggggcgtgc cgcatccacc
    23941 ggcgcgcggt gcgcaaagtg ggcgcgcacc gcccagccca tcagggccgt cacccccgct
    24001 tcggtgatgg tgaccgcgat ggccgcgccg ggcgcgccgt agaccggcgc cagcagcgcc
    24061 agcaggccca ggttgacgac gcccgaaacc agcaggatgc gcgagaacag cttctcccgc
    24121 ccgagcggca gcagggtctg gatgccgaac acgtttgaca ggccgatcag gcagggcaag
    24181 ggcgccatcc agcgcaccac ctcgaccgcc gcctcgaagg cctcgccata cagcagccgg
    24241 acgaggaccg gcgcgaggac gaagagcccc accgacaccg cgaaggtgaa cgcgccctgc
    24301 cacttgagca gacgccgcag cgtgcccaag gccgcggcgc ggtcatgatg catgaggtgg
    24361 ctcacccgcg ggaagagcgc ctgggacacc ggcgccgtgg cggcctgggc agccttcagc
    24421 agcttgtcgg cggcagcgaa atggcccacc gccgccgtcc ccgagagcca gcccagcaac
    24481 accgtgttgg aggtggtgta caggctgatg gccgccgtcg agacgaaaac gtgccagccg
    24541 tccgccagtg cggcccgcag gtcggcgagg gccggccgct gcatcgccac ggcccgcgag
    24601 cgggccagct ccgccaggca ccacagtccc gacagcaggc ccggcgcggc cgtcagcagc
    24661 atggccgcag gcagatccgc cggcccgcgc acgccggcga caatcagcgg aatcgccagc
    24721 gccttgggca gcagggtcgc cagcgcggca aaatcggccc gctccacgcc ctggaagtac
    24781 caggtgggcg tcagcacgct gccgatcacc gccagatagc ccagcgccag caggccggag
    24841 atttcaccca gcgttgagct cagggccgcc aggacgagca gcatcagccc gccgacaaag
    24901 gcgagcagcg ccttcacgcc cagcgttgcc cagaaggtcc gcgagcgcgc ggccttgtcg
    24961 tcccgttgca cggcgatctg gcgggtggcg ctcaggttga agccgtagtc cgccagcagc
    25021 acgaaatagg cgacgaccgc cagcgcaaaa ttgaggcggc cgtagccgtc aacgcccagc
    25081 gcccgggtga gccagggctg ggtgagcagc ggcaccgcga agttcgcggc ctggatgacg
    25141 tacagggaca cgagggtgcg ccgcagggaa gccatcggcg ccgatcaacc ctgggccgcg
    25201 gcgatgcggt cgaacacctg cagggcgttg gccaccgcgt catccatgtc gaagtaacgg
    25261 tactgggcca gacgccccac cgccaccacc cgggcatgac cgccaaggcg gtcggcgtag
    25321 gcggtgtagg ccgccttgct gcgctcgtcg aagatcgggt agtagggcac attcgcctcc
    25381 ggatcggcgg gatcgtaggc ctggggatat tcgtacacga tgctggtgcc cgcgcactgt
    25441 tgcccggtga ggtgcttgaa ctcggtgatg cgggtgtagc ggtgctcgtt ggggtaattc
    25501 accgtggtgg cgggctggaa gaccggcacc gcatggtgct ggaagtcgaa gcggatggag
    25561 cgataaggca gagcgccccg gtcgcggccg aagagttcgt ccaccatgcc ggtgaacacc
    25621 agcaggccgc caaagggtgc gccctcgaac tgcaccgcgc cggtgtccgg atcgaaggac
    25681 cccaccgcgt tgaacggcgt atcgaggcgc accgtgatgt ggggatgggc cagcagcttc
    25741 tcgaagagct tggtatagcc ctccgccggc acggcctggt acggatcggt gaagtagcgg
    25801 tcgtcccgat ccaccaccac cggcacccgc ccggtcactt ccggcgagat ctcctcgggc
    25861 ttcacgcccc actgcttggt ggtgtagttg aggaaggcct tgcgatagac gaagtcggcc
    25921 agctcccgca gcagcggctc gtcctcttcc ttgaggcgca ggatgggcac ccgagcgccc
    25981 aggccgaagc gggacaccag cgcctcacgc atgcacgtcg cggtgtcggc gtcgaagagc
    26041 cggtcgatgc tgttgaggtt gaaggggatc ggcaccagct gtccgtcaat gctggacagc
    26101 accttgtgct cgtagggccg ccattcggta aaggcggaca ggtaatccca cacgcgggga
    26161 ttgtccgtgt ggaacaggtg ggggccgtag cgatgcacca gcacaccgtg ggcgtccggc
    26221 tcgtcgtaac agttgccgcc gatgtggggg cgctgctcca gcacgaggac cttccagccc
    26281 agttgcgacg cgaagcgctc cgccagcacg gcgccggaaa acccggcccc gacgatgatc
    26341 gcatccacac tcatggttct ctccttgcgc cccgcgggcg gcttcgccct ggcgaactcg
    26401 tcaatacgcc ccgtggccgc tcaccaccgt gcggatcgtc ttgcacagga tggcgatgtc
    26461 gtaccacagg ttccagttct tcacgtacca cgcatccagc gcgacgcgct cgtcgtaggt
    26521 cgtctcgttg cggcccgaga cctgccacag gccggtgagg cccggccgcg cctcgagata
    26581 gaaggccacg cgctcgccgt agcgttccag ttcggcctcg accaccggcc gcggccccac
    26641 caggctcatc tcgcccttca gcacgttcca cagctgcggg atctcgtcca ggctggagcg
    26701 ccgcagaaag gccccgatgg gggtgatgcg cggatcgttc ttcagcttga aatcccggtt
    26761 ccactcctcc cgcgcctggg gatcggacgc cagcaggcgc gccagcacct cggccgaatc
    26821 gtgggtcatg gtacggaact tccagcactt gaagggcacc ccgtccaggc caatgcgctc
    26881 gtggccgaag aacaccgaac ccggcccgcc ctgacgcttc acctgcaccg cgatataggc
    26941 cagcagcggc gacaggagcg ccagtccgca ggccgacccg gcgatgtcga acatccgctt
    27001 gagccagcgc agcgggcggg acgccaggtt gttgcgcacc cgcagcatca gcacctcgtg
    27061 gctgaagaag tggttggcgc tcagcccgaa caaaggcagc ccggcaatcg gcgggatgat
    27121 gtgcagatcc ttgtaatgca gggtcagcag ctccacctgc ccggcgattt cctgcagttc
    27181 cggctcgctc accgccaccg tcacatgggg atgtccctga tccgccagcc agggcaggag
    27241 cttgtccggg cgcgccaccg gtaccgtgac gccaggcgcc acgaccagct cggccggcgc
    27301 ctcgggcccc acggcgacga aggcgtgcac ctcgaagccc agcagatgct cgctgcgcag
    27361 ggcctgccag gcggccacgg cgttctcacc gacgcccacg atgacggtcg gcagttgcca
    27421 ggagccggcc cgcatcagcg cggacttcat gccgttacgg aacaggggca gcagcaccag
    27481 cgccaggccc cacgataccg gccacaggaa gcgcgacacc ggccacttgg ccagcagcac
    27541 caccatgccg ttgagcagcg ccgccagcag caggctgcgg atgatctcca gcaactcgtc
    27601 ccagaagggt tgtcgccggt ggtaatgccc acgccacgcg aaggtgacga gcgccagcac
    27661 cagcagcaac aggaaggcca gcgcctggtt caccccttcc gccgcccacc actgcgcaaa
    27721 actcggtccg atgcgatgcc cgcgcaacca cagcagcagg agcagcgcca agccgaaggc
    27781 cacccccaag gccagcgcat cggccagcac cagccagccg cgcacttccc cggcgggccg
    27841 ctgcgggcgc ctcatgtcag ttctggagtc cataggcccg gtacagtgcc tccatcatcc
    27901 ggtcgaaggc gaaatgcgcg cgcacgaagg cctgggcccg tgccccggtc gcctggcgca
    27961 gggccggatc gttcaccagc gggccgagcc gcgcgacgaa ggccgccgcg tcctccggca
    28021 ccagccagcc cgtgtccccg tcctgcacca gatcccggtt gcccaccacc cgcgtggcga
    28081 tcaccggcag gcccgcgccc atggcttcga tcaccgccac cggcaaccct tcccagcggg
    28141 acgtctgcag atagatgtcg agccggccga gctcctccag cgcacgggcc cggtccaccc
    28201 agccggtgat gtgcacgcct gccgcccgca gcgccgcctc gccggacgcc tcgccgccgc
    28261 cgatccagac gaactccacg ccgtcacgtc cgctgcggcg ggccacttcg gcgaagagct
    28321 ccggatggcg cgccgtggtg acgcgcccca ccgtgccgat gcgcacgacg cctgtcccgc
    28381 cccgcgcctg gggcagaatc gccaggtcca ccgcattggg caccaccacc acgcgccggc
    28441 tcagatgggt gcggatctgc tccgcctccg agggcgaaca ggcgatgaag gtcaccggaa
    28501 tgcgcgccag cagcttctcg agggtcagga acaccgcgtt cttgatgcgc cccgccgcct
    28561 tctgcaggaa ggacagccca tggggcgaaa aaaaccagcg cggccccgga cagaccaggc
    28621 tcagcaggcg ccccagcgca ccggccttgg acgagtgcag atgcaccacg tcgggccgcc
    28681 agtcccgcag gatgcgccgc agggccaccg ccgacgccca gtccgtcgcc gggcggatcg
    28741 cccgggtcat gggcacatga cgggtcgcga tgcgcggatc gaagagcgcc cgccagttcg
    28801 ccggcgtctc ttcccgcgtc gaatacacca gcgacacccg gtgcccgtcc gccgcctggc
    28861 ggttggcgat ggccgacacc atggacaggg tgccggcacc gaaggattcc accacgtgca
    28921 ggatatgcgc cacgatcccc tcaacccgcc cgcgcgcccc ggcgcagc
//
LOCUS       DAOQMM010000041        28415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_478_length_28415_cov_47.175353, whole genome shotgun sequence.
ACCESSION   DAOQMM010000041 DAOQMM010000000
VERSION     DAOQMM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 28415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28415
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_28415_cov_47.175353"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PLH85_08710"
     CDS             complement(<1..946)
                     /locus_tag="PLH85_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64683.1"
                     /translation="MKKLAIVAALAALGAASQANAAFTSISVDPDGAGGAYGTISITS
                     LNWLAGNAVAIGAGGLAGQSLADYNTALATTGETIHTYYQAQLNTFVSNSGGSSTTYT
                     PVGQWTVVADIVENQMASGAASVSLTPVGGTVSIYYNATKTANDITGTGFTDGTKILQ
                     GSIVGGSGSFQNTTALNDLLVSLGLIPSNPTPITQLDNFGADNKPGVTSIQGNGQSTL
                     KVDIGATAGDFVDANFFKTNITTFDFLTTGLEDANDTGQLVDPFNQANPSAMVNGYTP
                     VYGAGNVNGVLAPVSGGVFTQNSDFQFQTTNVTSFNAIP"
     gene            complement(1161..2336)
                     /locus_tag="PLH85_08715"
     CDS             complement(1161..2336)
                     /locus_tag="PLH85_08715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB64684.1"
                     /translation="MNPTAPESHPAPTHGGVALPGLTGLRAYLAFWVIAFHIIAGLVI
                     DKFPVDSLLPPAALGFANAGMWGVDGFFILSGFVLSHAYGRSMSTGFDLHGTLDFLGN
                     RLARIYPAHFAVLAGYGLVKLAGLSFLYESCGSPYQPVACERFGIEALFRHLTLISSW
                     GWNPPLTWNPPAWSISSEWFAYLCFPLLIIAARRLHTAGAALALATVALLAMLGTLYL
                     HGFHPFGEEPEAGLIRICGEFVCGVSLYRFYHTPLYARLPWHWIGSIAAVLVLSNVLG
                     QVQWLLPFVMALIVLSAAQPRGPLSRLLSTRPMVWLGNVSYSLYMVHLLVLELLGILF
                     PEPPTPPEQMGPLDVLGTIIFMVTLCVVGATALYYLVEEPARVRVKSLVTRALGQRS"
     gene            2517..3758
                     /locus_tag="PLH85_08720"
     CDS             2517..3758
                     /locus_tag="PLH85_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB64685.1"
                     /translation="MHPQLTRALLDLSLAFRNVIRHRQRAAFALIIIGGGVIALLLAS
                     GFIQWVLDIQRESTIRSQLGHIEVVRPDYFGKGISNPFAYLLPQNHPAEEVIRGHKEV
                     DALAPRLNFVGLLAFGDATLSFVGDGVDPAAEKEFADYVFVEDGERLDDADPTGVIVG
                     QGLATNLGVKTGDRIVLMVSTAKGGLGATDAHIRGIFFTAEKMYDDSAIQMPITLARK
                     LVKVKGATSWLVVLHDTEDTDTVLGSLRAKLDPKTFELVPWYDLADFYKKTVQLFSRQ
                     VMVVKFLIGMIIVLSISNTLSMAVVERTGEIGTAMALGVRRHSILRLFLMEGLVLGAL
                     GGSVGALIGWLLSVVISAVGIPMPPPPGMTTGFTGQITVNFAMVVEAVLLAVVTTLLA
                     SAFPAWKASRMVIVDALRHQR"
     gene            complement(3759..4958)
                     /locus_tag="PLH85_08725"
     CDS             complement(3759..4958)
                     /locus_tag="PLH85_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB64686.1"
                     /translation="MSRKKILLMAHDVTLAHVGRPLKLGELLHADGHDVVLATSADSA
                     RFLAGFPGRTRTLAPTGKARFIDNLAKGRPVFDYATLKRYAEEDEAVLSAEKPDLVIG
                     DFRITLSITARRLSIPYATLTNAYWSPAFTPRFIVPDLPMVRFLGVRLSQAIFDAVRP
                     LAFALHCRPMDALRRHYGLPPLGPDLRRIYTDADLALFSDIPEVYGLYGDIPGGHFLG
                     PVRWSPEVPLPDWWAALDPNKPLIYVTLGSSGDGRLLPRLLDRLARPGLQLAVATAGA
                     TVGVTAPDVFCADYLPGDAVAARAALVICNGGSPTCAQALAAGVPVLGVPGNLDQYLN
                     MHYLEGQGVGLTLRNRELDGPNLKRTVDRLLGEPSFRRAAQSLAATLSRYDLAERLRE
                     ALRRLTA"
     gene            complement(5030..5617)
                     /locus_tag="PLH85_08730"
     CDS             complement(5030..5617)
                     /locus_tag="PLH85_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64687.1"
                     /translation="MSHLPLIRPGPAHRSVAAALALALLGGGAAYSTASRHADAEARL
                     LQTQHALTDVRTRLQVAEDTLAARQKQLAAAAQLRAQGLSAGPDRPAWLKAVRAELQA
                     QHLASLDVELGPHRPLDGAADFDASPLTLRGPLAHEGQLLRLLDVLDRQPGTLLLPRR
                     CRVSRLDTTDAPDERLHADCQLEFVTLRPDPARAP"
     gene            complement(5614..7038)
                     /locus_tag="PLH85_08735"
     CDS             complement(5614..7038)
                     /locus_tag="PLH85_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64688.1"
                     /translation="MALCLLHLCADGATAYLRTTGAYRASARFGQADAAFSAWLGAHP
                     RMTLRVMADLPDEQFLIDALPILRGQERHQLIERRRSRLFASTPFVGIRPLGRADGRE
                     TLLFHALTRPAALQPWLDALRHAGARLDGLHSVADAMAALRPHLPSGAPTCAIAGLTH
                     TGLRLTALVDKHPQWSRLVPATAVTDLSTDLPNICNAARDHLVARRLLHPEHPLPVVL
                     LPGLETFAGALGAGLQAIAPSATELARRCRLPPPGPHTDALPYILYGAGRRGLPQFVP
                     PDRLTARPLRAVDQAILLAGATAAAIGAAVAIKFMADTRALSDTDARLTAEVVATEDR
                     LSRVNAALPPPPAGDPLAATQSLSRLQDARNWPTRLLDRLSDLLEAEPDSELKSVEWQ
                     LPPDGPPVAVAEIALPAALADTPPALAARLDRLRAAVISAGYVQASLERVPDELDTTR
                     SVRLRPAPEAQPRVVVRFSPAPPP"
     gene            complement(7041..8243)
                     /locus_tag="PLH85_08740"
     CDS             complement(7041..8243)
                     /locus_tag="PLH85_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB64689.1"
                     /translation="MTRFRYRAADAHGQLREGELEAHHVDDLDLRLRRLGLDLIRATP
                     ASRTPRGGRRGVPRRELINFCFHLEQCQRAGVPILESLADMRDSTPHPVLRDTLGALV
                     DGIQGGLSLSRALERHPALFDGVFVSLVRAGEQAGQLPTVLHALAEGLKRDDELAAFA
                     RRIAIYPAIVGTVIAAALVVALLYVVPELARLFHATGQPLPLQTRLLIGLSNLLIDHG
                     RLLAVLALAAVAALRFALATRPDFRRWYDGAQLALPVLGEIRRKIILARFTALFAMMY
                     GAGITIVDALAAAEGVVGNTALRAALQRIADAIGDGRRVSDAFADTPAFPPLVARMLR
                     VGEHTGALDTALANVGYFYERDVREAIARVQASLEPALTLLLGGLLLWVMSAVLLPVY
                     DLATRMKL"
     gene            complement(8240..9916)
                     /locus_tag="PLH85_08745"
     CDS             complement(8240..9916)
                     /locus_tag="PLH85_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNB64690.1"
                     /translation="MNGQPLGQALLQRGLISADQLAIALREQERSHEAIGRVLVRLGF
                     LSEATLRDALSHALGRRSVDLDTLVPDPAALTLLPRDLACRLNVLPVSLDSTSRVLTV
                     AAARIGDPILLDSLQAQLPAGLHIEPVLAAESQIARAIDQLYGHALSIDGILHEIEAG
                     AAGPVEQAQPVVRLVDALLVDAVKSDASDIHFEPEPGFLRIRYRIDGLLRQIRALHTS
                     YWPAMAVRIKVMAGLNIAETRMPQDGRISLHVNGRPVDFRVSTHPTLHGENIVLRVLD
                     RQKGIVPLDRLGLADTQLDTLKLMIARPEGLILVTGPTGSGKTTTLYSILNHINAEGI
                     HIMTLEDPVEYPMPLIRQTAVAEAAHLDFGNGIRAMLRQDPDVILVGEIRDTDTARMA
                     LQAAMTGHQVYSTLHTHSAVGALPRLLDLGVAADILAGHLVGIVAQRLLRRLCPHCRE
                     ARPAEDYELHLLAQPADAPRPRLHHPRGCPQCQFQGYRGRMAIMELLRIDHRLDELVA
                     RRASPRELLAAARDQGFRSLAEDGSRRVLEGHTSLEELARVVDLTDRMRP"
     gene            complement(9913..11145)
                     /locus_tag="PLH85_08750"
     CDS             complement(9913..11145)
                     /locus_tag="PLH85_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB64691.1"
                     /translation="MSLLLEVLRKHEAERRGETTPPGASAPPPAPAPAPADDIHTEQA
                     SQTPAADARSPVMCPSPLSLEPLNAPPTGNSQPPAPPPFTTAPQPIHPPPPGMAPTTP
                     ATTPHSHARWAWIAGLTVAGLAGLAGWVWSETRPTGLSPIQLAQARGAEASTEVQPPV
                     SAPPPETPPETLPAAPPAVARSRTTSPAARPPSPARTDPPDSDAHAALRFEPRPMPVA
                     SPQPDLLRAHDAYTRGDLTAAHQHYQAALSLDPTNPDAHDGLGALALHSGLHVEAARH
                     FRLALAARPTDSAALAGLASLPGNDSRQAESRLRSSLDADAGDATAQFALGTVLARQQ
                     RWAEAQHAFFRARSLAPEDPDIAYNLAVSLDHLRQPGPAAAHYRLALQLAERHLAGFD
                     RAACAARIDALAAPDNAP"
     gene            complement(11142..12770)
                     /gene="mshL"
                     /locus_tag="PLH85_08755"
     CDS             complement(11142..12770)
                     /gene="mshL"
                     /locus_tag="PLH85_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HNB64692.1"
                     /translation="MVHNVRLQDLLFALARDARLNVDIHPGLNGTVTLNAIDQTLPQI
                     LTRLARQADLRFELDGTHLAVMPDSPYLRTYRVDYVNLSRSVTGTVSTNTQIATSGSG
                     SAVSGSTTLQTAALPNGGNVSATRIENASRNQFWESIERNIRDLLRETDKLLPEGSSD
                     TVIENLETTSTSGTGVQPSATAARSRTRTTSASPQAGIAASPNPALLQNSATTVVHKA
                     TFREAASVIVNRETGVLTVRATGRQHEKVQEFLDQVVAAARRQVLIEATILEVGLSDT
                     YRQGIDWNRVLSSGTRFGITGPTLGSSAGNAVNPFSVAYKSADGIDSTIRLLEGFGTV
                     KVLSSPKLSVLNNQTAVIKVVEEYVYFNVKADTTQTTNAGALTTVTTTPQAVSVGLVM
                     TVTPQVSEADEIILNVRPTISSIADFRRDPNPNIPATIPNLVPQIRTREIESVLRIEN
                     GETAVLGGLMEDRVDFKTGRVPGVGAVPLLGEVFTTRDNLVQKTELVIFLRPVVVRDG
                     NLLAASPLRNSLPRDDFFRRGPLPGTRNFPDTTP"
     gene            13351..15309
                     /gene="acs"
                     /locus_tag="PLH85_08760"
     CDS             13351..15309
                     /gene="acs"
                     /locus_tag="PLH85_08760"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNB64693.1"
                     /translation="MTSETTQKLYYPSEEVVKNAAVSGMEAYKALCAEAEADYEGYWG
                     KRAKELIEWQTPFTQVLDESNTPFFKWFADGKLNVSYNCLDRQVKNGLGDKVAMIFEG
                     DKGDVTKVTYGDLLTRVSKFANALKSLGVKKGDRVVIYLPMSIEGVVAMQACARIGAT
                     HSIVFGGFSAQSLADRVNDAGAVVLITSDGQNRGGKALPLKAIADEALASGCPSIQKV
                     LVVKRTGGETAMVEGRDVWADDIVAQQSDVCEPEWVEAEHPLFLLYTSGSTGKPKGVQ
                     HSTGGYLLHAILTMKYTFDIKPNDIFWCTADIGWVTGHTYITYGPLACGATEIVFEGV
                     PTYPDAGRFWKMIQDHKVSVFYTAPTAIRSLIKASDTTPAVHPKNYDLTSLRILGSVG
                     EPINPAAWEWYYENVGGSRCPIVDTFWQTETGGHMITPMPGATPLVPGSCTLPFPGIQ
                     AAVVDETGTDVPWGQGGILVVKRPWPSMIRTIWGDPERFVKSYYPADFQGKLYLAGDG
                     AIRDKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVAHEKVAEAAVVGRPDDLTGEA
                     IVAFVVLKGARPSGDEAAKVVKELQNWVGQEIGPIAKPKDIRFGENLPKTRSGKIMRR
                     LLRQLAKGEEITQDTSTLENPHILDQLK"
     gene            15616..15804
                     /locus_tag="PLH85_08765"
     CDS             15616..15804
                     /locus_tag="PLH85_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64694.1"
                     /translation="MKATLMGQRLVAVFVLGCVLANFPVLSIFNDDGAWQGIPLLYLY
                     VFGLWAAVIVLMAWIVER"
     gene            15846..18602
                     /locus_tag="PLH85_08770"
     CDS             15846..18602
                     /locus_tag="PLH85_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB64695.1"
                     /translation="MLSGTTVVAVSFLYLAALFGVAYYGDKRADAGRSVIANPTIYAL
                     SLAVYCTTWTYYGSVGRAASSGIGFVPVFLGPTLVVGLWWFVMLKMIRIGKSNRITSI
                     ADFIASRYGKSHLLGGLVTVIAVLGVIPYIALQLKAVSGSFSILIHPQGLLTPIRTGA
                     LPWMTDTTFYVALMMAAFTILFGTRHLDATERHEGLVAAVAFESLVKLVIFLVVGGFV
                     TFGLFDGFGDLFSQAAEDPALRALMVVETGGNSYGNWFALTVLSMLAVMFLPRQFQVA
                     VVENVNEKHLRRAIWLFPLYMMLINVFVLPIALGGLIYFRGQAVDADTFVLTLPLASG
                     HPLLALLVFIGGLSAATGMVIVETIALSTMVCNDLVMPALLRFSRGALRGHNDLSGLL
                     LAIRRGAIVLILLLAYLYFRLAGEAHALVSIGLISFAAVAQFAPAMLGGMYWRGGSRA
                     GALAGLSAGFLVWGYTLLLPSFAKSGWLPGDFLQGGLFGLEFLRPQELFGLRGLDPMA
                     HSLFWSLFANVGAYTWVSLLRPPNAVEAGQATLFVDVFRQGRPSGGAFWRGSARVGDL
                     LPLIARFVGLERAQALFSSYARERGVERVADLEADAGLVHFAETTLAGAIGGASARVM
                     VASAVKEEPLGLDEVMHILDEASQVRAYSQRLEQKSRELEAATAELRHANDRLKELDR
                     LKDDFMSSVTHELRTPLTSIRAFSEMLLEDPRIDLADRKRFLGIIVAETERLTRLVNQ
                     VLDMAKIESGHAEWHNGEVNLVEVVEQAVASTSQLFKDRGVSLELDLPASLPVLLADR
                     DRLIQVMLNLLSNAVKFVERDGGQVRVKLAETSDGVQVDVSDNGPGLTLAEQTVVFEK
                     FRQGGNTMTAKPQGTGLGLPISRQIVEHYGGRLWVESAPGEGARFSFFLPCGRSTEAQ
                     ERAG"
     gene            18683..19057
                     /locus_tag="PLH85_08775"
     CDS             18683..19057
                     /locus_tag="PLH85_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64696.1"
                     /translation="MTKKILIADDEQNIVISLEFLMKREGFAVCIANDGQEALDKVRS
                     ESPDLVLLDVMMPRKNGFEVCQEIRADGALQGVRILMLTAKGRETEVSKGLAMGADAY
                     MTKPFSTKELVARVKSLLELPA"
     gene            19252..21219
                     /locus_tag="PLH85_08780"
     CDS             19252..21219
                     /locus_tag="PLH85_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB64697.1"
                     /translation="MKSFVQAHVAAPALLADEIGLIVGTDPARRLKAQADPDLNSIAD
                     AVNALADQRRSLQDDVEARIREARLSVEEERNRLAALMSELTQSVVVCNLDGRVLLYN
                     NRARLQFKALSDAPAAAGGGEIIGLGRSIYAVFERNLIAHALESIQERLRRQSAQPVA
                     NFVTTTRAGQLLRVQMAPVLSVGEGSAEPAVTGFVLMLDNITRNFESETRRDQVLHTL
                     TEGSRSALANVRAAVEMLEFPDLDADLRERFLTVIRDEVRGMSERLDKTASEFADSLK
                     TRWPLDEMLGADLVAAAQRRIEKKIGLPTKLEDVDDSVWVKVDSFSLLQAVGYLASRL
                     SDEFEVREVRLRLTSAGRLVHLDLIWSGQAMSTETVMAWELEPMSFAGESSPLTVRDV
                     IERHDGEIWLQREKTRHRAFFRVLIPSAAPQEQVESSAYLKGDSRPEYYDFDLFKHSE
                     ASDALDDRLLSELTFTVFDTETTGLNPSEGDEIIQIGATRIVNGKLLKSESFEQLVDP
                     LRDLPEASTKIHGITPEMLEGQPALDKVLPAFHAFSQDTVLVAHNAAFDMRFLQIKEE
                     SLGVRFTQPVLDTLLLSAVIHPAQESHRLEAICERMGVNVMGRHTAMGDAIVTGEVFL
                     KMIPLLAEAGIRTLGDARRASEKTYYARVKY"
     gene            21289..22722
                     /locus_tag="PLH85_08785"
     CDS             21289..22722
                     /locus_tag="PLH85_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNB64698.1"
                     /translation="MSSSRESRPLSGDGQALASLLRRAPVSVPPESPVRDVLKRMLAA
                     RVGSVVVADVDTGFPLGVLTHRDVLERVVLAAGDLDVPVASVMTGGVMSLPLGANVHL
                     ARLLLAQRRLRRVVVVDADGRLAGVVSRGDLYAQRQPCADDLVSRIRAADHVEALRDA
                     ARGVRDAAARWLDDGAGAGPVAEWIALLNDLVAQAALDLALRDVELPLVRWCWLAFGS
                     EGRLEQTFETDQDNGLIFEVPDDVDADAMRERFLPWAQRVNDVLDTCGFARCAGGIMA
                     GNPTWCLSVGEWRERFLSWMLSADPEALLNATIFFDFRPLAGDWELAGRLQEWLLART
                     AESELFLRHMAGNALRGGPPLGRIRDFAVDRKTGRLDLKRDGSRPFVDVARIYALALG
                     LRETATADRLRGAGRLLNWSRREIDAVVEAFHCIQRLRLDRQQRGQGPANLIAPEELN
                     ELERATLKEAFRQARKLQARLQIRYQL"
     gene            22778..23044
                     /locus_tag="PLH85_08790"
     CDS             22778..23044
                     /locus_tag="PLH85_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HNB64699.1"
                     /translation="MAVDSAGRRAYWRANLALTAVLLVIWAAVSFVPGWYAEDLEAYS
                     VFGWPLDFYMAAQGSLIVFLVIVWLYDRCMRRLERRLGVPDERD"
     gene            23055..25127
                     /locus_tag="PLH85_08795"
     CDS             23055..25127
                     /locus_tag="PLH85_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB64700.1"
                     /translation="MKSSHSYFVRLGRYYGWYSAGFFVLIGLLAIAEQLGMTPRWIGQ
                     IFLLATIALYAAIGVMSRTSDVSEYYVAGRRVPAVFNGMATAADWMSAASFIGLAGTL
                     YYTGFEGLAFVTGWTGGYVLVALLLAPYLRKFGQFTVPDFLGARYGGHAARIVGVVAA
                     VLASFVYVVAQIYGVGLITSRFIGIEFEIGVFVGLAGILVCSFLGGMRAVTWTQVAQY
                     LILIVAYLVPVTILSYKVTGVPLPQLTYGGVLKDLGNKELELFSEPRENEVRTQYRYR
                     ADDYARKIIDLPDSLAAERTGQLQRVEELRTQGASARELAAAERALRDMPRTPDEARV
                     KWERARQESLVRAAPPVPHAQAHPGPDRAARDSARNNFIALAFVLMVGTAALPHILMR
                     YYTTPSVRQARNSVFWSLFFIFLLYVTAPAYAVFAKWEVYSGLVGTPIASLPAWVAAW
                     GRVGLVKIDDINGDGILQLAELSLNSDAILLLVPEIAGLPYVVSGLVAAGGLAAALST
                     ADGLLLTISHALSHDLYYKMINPEASTQRRLIITKSQLLIVAVCAAWVASLRPDGILF
                     MVGLAFSIAASAFFPALVLGIFWKRANRWGAVSGMAAGLGITLYYIVRTHPFFGGSLS
                     EAWFDIHPISCGIFGVPVGFVTIVVVSLLTPPPDAATQRLVDFIRMPDGIEDFPDRDE
                     KVFDAGGS"
     gene            25149..25239
                     /locus_tag="PLH85_08800"
     tRNA            25149..25239
                     /locus_tag="PLH85_08800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25183..25185,aa:Ser,seq:gga)
     gene            25376..25756
                     /gene="rpsF"
                     /locus_tag="PLH85_08805"
     CDS             25376..25756
                     /gene="rpsF"
                     /locus_tag="PLH85_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNB64701.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMIERYTTLVTSQGGQVHRLEDWGR
                     RQLAYPIQKIHKAHYVLMNIECDQPTIAELEHGFKFNDAVLRHLSIRMKRAVTAPSPM
                     MKEEKARSLTPAADEAKPAEQPAA"
     gene            25759..26109
                     /gene="priB"
                     /locus_tag="PLH85_08810"
     CDS             25759..26109
                     /gene="priB"
                     /locus_tag="PLH85_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HNB64702.1"
                     /translation="MQEAGRNSLELEGVLTEIGALRRTPAGIPVLACRIEHRSRQMEA
                     GRLRDVGVELQAVVVGDKTSLLAAAPLGSLLHVTGFLAAKSLRSRAPILHIDTIEFLE
                     GNRHGIQARNEAQG"
     gene            26075..26344
                     /gene="rpsR"
                     /locus_tag="PLH85_08815"
     CDS             26075..26344
                     /gene="rpsR"
                     /locus_tag="PLH85_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNB64703.1"
                     /translation="MAFKPAMKRKDDRGGRGLFKRRKFCRFSAEKIEEIDYKDVDILK
                     DFITENAKIMPARITGTKSGYQRQLSTAIKRARFLALLPYTDLHQ"
     gene            26360..26809
                     /gene="rplI"
                     /locus_tag="PLH85_08820"
     CDS             26360..26809
                     /gene="rplI"
                     /locus_tag="PLH85_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNB64704.1"
                     /translation="MQIILLDKVVNLGGLGDVVKVKDGYARNYLIPQGKAKRATATNL
                     AEFEARRAELERQAAEQFAAAQAEGAKLEGQSVTIARKAGVDGRLFGSVTNADIADAL
                     KAQGFAVEKSAVRMPEGPLKTVCEAQIVVSLHTDVTATITVTVVGEN"
     gene            26918..28309
                     /gene="dnaB"
                     /locus_tag="PLH85_08825"
     CDS             26918..28309
                     /gene="dnaB"
                     /locus_tag="PLH85_08825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNB64705.1"
                     /translation="MRNASLSEDPALASLRLPPHSLEAEQSLLGGLLIDNTVWDRVGD
                     IVSEVDFYRDDHRRIFRQIAKLIELGKPADVVTVYEALEKAGEAEHVGGLAYLGEIAN
                     STPSAANVRRYGEIVRERAILRKLVAVGDQIAASALAPSGKDAKTLLDEAEAKVFEIA
                     EAGARTTTGFVPIQPLLGQVVDRIQELYDRDSPSGITGVATGLTDLDEKTSGLQPSDM
                     IVVAGRPGMGKTSFALNIAENVAVECGLPVAVFSMEMPGTQLATRFLSSVGRLDQHRV
                     RTGKLTDEEWQRLTFALGKLHEAPIYIDETPGLNPTDLRARCRRLHRQCGRLGLIVID
                     YLQLMTSLKESDNRAAELSEISRSVKSLAKELHVPIIALSQLNRSLEQRPNKRPVMSD
                     LRESGAIEQDADIIMFIYRDEIYNPDSQDKGTAELIVAKHRNGSTGTIRMTFIGEYTR
                     FENYVGGAAVAEY"
BASE COUNT         4611 a         8313 c        10410 g         5081 t
ORIGIN      
        1 cggggatggc gttgaacgag gtcacgttgg tggtctggaa ctggaagtcg gagttctggg
       61 tgaacacgcc gccggaaacc ggggccagaa cgccgttcac attgccagcg ccgtacaccg
      121 gggtgtagcc gttgaccatt gcggacgggt tggcttggtt gaacgggtca accagctggc
      181 cggtgtcgtt cgcgtcttcg aggccggtgg tcaggaagtc gaaggtggtg atgttcgtct
      241 tgaagaagtt ggcatcgacg aaatcgccag cggtggcgcc gatatcgacc ttcagggtgc
      301 tctggccgtt accttggatg gaggtcacgc ccggcttgtt gtccgcgccg aagttgtcca
      361 gctgggtgat cggggtcggg ttggacggga tgaggcccag ggacaccagc aggtcgttca
      421 gggcggtggt gttctggaag ctgccggaac cgccaacgat ggaaccttgc aggatcttgg
      481 tgccatcggt gaagccggtg ccagtgatgt cattggccgt cttggtggcg ttgtagtaga
      541 tcgacacggt gccgccgacc ggggtcaggc tgacggaagc tgcgccggaa gccatctggt
      601 tttcaacgat gtcagcgacg acggtccatt gacccaccgg ggtgtaggtg gtggaggaac
      661 caccggagtt gctgacgaag gtgttcagct gagcctggta gtaggtgtgg atggtctcac
      721 cggtggtggc cagggcggtg ttgtagtcag ccagggattg gccggccaga ccgcctgcac
      781 cgatggccac ggcgttgccg gccagccagt tcagggaggt gatggagatc gtgccgtaag
      841 caccgccagc gccatccggg tcaaccgaga tggaggtgaa ggccgcgttg gcctgggagg
      901 cagcgccaag ggcagcgagg gctgcaacga tagcgagttt tttcattaga ggctcctggg
      961 ttgaaggttt gagatcatcg aagttatctc acgggaacta ttaagcagct gccgtgccag
     1021 tttcttaatt ggctgaaatg caaatgtttt ttgccagcac gctggcgcct gcgaggtgga
     1081 ctgtaaaaaa tgtcgacagg atggtccggc gaaaaccggt aatttgtcac ggtcgtgctt
     1141 tccgttacgg aaggtgacat tcagctgcgt tgcccgaggg cgcgtgtaac cagcgacttg
     1201 actcgcacgc gggcaggttc ttccacgaga tagtagagcg cggtggcgcc gaccacgcac
     1261 agggtgacca tgaaaatgat cgtcccgagt acgtcgaggg gacccatttg ctcggggggc
     1321 gtgggcggtt ccggaaagag gatgcccagc agttccagca cgaggagatg gaccatgtag
     1381 agggaatagg acacgtttcc cagccacacc attgggcgcg tggacagcag acgcgacagg
     1441 gggccgcgtg gctgggcggc ggacaggacg atcagggcca tcacgaaggg cagcagccac
     1501 tggacttggc cgaggacatt gctgaggacc aggacggcgg cgatgctgcc gatccagtgc
     1561 cacgggaggc gggcgtacag gggcgtgtgg tagaagcggt acaggctgac gccgcacacg
     1621 aattctccgc agatgcggat caggccggct tcgggctctt cgccgaaagg gtgaaatccg
     1681 tgcagataca gcgtcccgag catggccaac agggccaccg tggcgagggc gagggccgcc
     1741 ccggcggtat ggagccgacg ggctgcgatg atcagcagcg gaaagcacag gtaggcgaac
     1801 cattcgctgc tgatcgacca ggccggcgga ttccatgtga ggggcgggtt ccagccccag
     1861 gacgagatca gcgtcaggtg ccggaacagg gcctcgatgc cgaagcgttc gcacgccacg
     1921 ggctggtatg gactgccaca ggattcatac aggaaggaca gcccggcgag cttgaccagc
     1981 ccgtatccgg ccaggacggc gaaatgggcc gggtagatgc gcgccaggcg gttgccgaga
     2041 aaatccaggg tgccgtgcag atcgaagccc gtggacatgc tccggccgta ggcatgggag
     2101 aggacgaatc cgctcaggat gaagaagccg tccacccccc acatgccggc gttggcgaag
     2161 ccgagggctg caggcgggag caggctgtct accggaaact tgtcgatgac gaggcccgca
     2221 atgatgtgga aggcgatcac ccagaaggca aggtaggcac gcaggccggt caggccgggg
     2281 agggcgacgc cgccgtgggt gggggccggg tgggactccg gagcggtggg gttcatggac
     2341 aatccttctt tgcgggcggg ccgggacgac cggacatgcc gggcagttta cagggcgtca
     2401 ggtccttgcg gcgcagggcc aggccctgac gcgtgtgcga aggtgcggcg accggcgggt
     2461 gccgtggcag gggaataggc agtatgattc cgtcgtttcc atccgacggc ttttccatgc
     2521 atccgcaact gacccgggca ctgctcgatc tgtccctggc gttccgcaac gtgatccgcc
     2581 atcggcagcg ggcggcgttt gcgctgatca tcatcggtgg cggggtcatt gcattgctgt
     2641 tggcgagtgg attcatccag tgggtgctcg acatccagcg tgaatcgacg atccgctccc
     2701 agctcggtca catcgaggtg gtgcggcccg attatttcgg taagggcatt tccaatccct
     2761 ttgcctacct gctgccccag aatcatccgg cggaggaggt gatccgcggg cacaaagagg
     2821 tggatgcgct ggcgccgcgc ctgaacttcg tcgggctgct ggcctttggc gacgcaaccc
     2881 tgtctttcgt cggcgacggt gtcgatcctg cggcggagaa ggagtttgcc gactacgtgt
     2941 tcgtggagga tggcgaacgc ctcgacgacg cggatccgac cggcgtgatc gtcgggcagg
     3001 ggctggcgac caacctgggg gtgaagaccg gagaccggat cgtgctgatg gtgagtacgg
     3061 ccaagggcgg actgggggcg accgacgccc atatccgcgg catcttcttc acggccgaga
     3121 agatgtacga cgacagcgcc atccagatgc ccatcacgct ggcgcgcaag ctggtgaagg
     3181 tcaagggggc cacctcgtgg ctggtggtgc tccacgacac ggaggacacc gacacggtgc
     3241 tgggcagcct gcgcgcgaag ctcgacccga agacctttga actggtgccc tggtacgatc
     3301 tggcggattt ctacaagaag accgtgcagc tgttcagccg ccaggtgatg gtggtgaagt
     3361 tcctgatcgg catgatcatt gtcctgagca tctccaacac cctgtcgatg gcggtggtgg
     3421 aacggaccgg cgagatcggc accgcgatgg cattgggcgt gcgccggcac agcatcctgc
     3481 gcctcttcct gatggagggc ctggtgctcg gcgctctggg gggctcggtg ggcgccctga
     3541 tcggctggct gctgtcggtc gtgatctccg ccgtcggcat tccgatgccg ccgccgccgg
     3601 gcatgaccac gggctttacc ggacagatca cggtgaactt tgcgatggtc gtggaggctg
     3661 tgctgctggc ggtcgtgacc accttgctgg ccagcgcctt tccggcctgg aaggcgtcgc
     3721 gcatggtgat cgtggatgcc ctgcgccacc agcgttgatc aggcggtgag gcggcgtagc
     3781 gcttcgcgca ggcgctcggc caggtcgtag cgggacaggg tggcggcgag ggactgcgca
     3841 gcgcgcctga atgagggctc ccccagcaag cggtcgacgg tgcgcttcag gttcggaccg
     3901 tcgagttccc ggttgcgcag ggtcaggccg acgccctggc cctccagata gtgcatgttc
     3961 agatattggt ccagattgcc gggtacgccg agcaccggca cgcctgcggc caaggcctgg
     4021 gcacaggttg gactgccacc gttgcagatc accagggcgg cgcgggcggc gactgcatcg
     4081 ccgggcaggt agtcggcgca gaacacgtcc ggtgcggtca cgccgaccgt cgcgccggcc
     4141 gtggcgacgg ccaactgcaa cccggggcgg gccaggcgat ccagcaggcg gggcagcagg
     4201 cgtccgtcgc cggagctgcc aagggtcacg tagatcaggg gcttgttcgg atcgagggct
     4261 gcccaccagt ccggcagcgg gacttcgggt gaccagcgca cgggtccgag gaagtggcca
     4321 ccgggaatgt ccccgtacag tccgtacact tccgggatgt cgctgaagag ggccaggtct
     4381 gcatcggtgt agatgcgccg caggtccggt cccaggggtg gcaggccgta gtgtcgacgc
     4441 aaggcgtcca tgggccggca gtgaagtgcg aaggcgaggg ggcgcacggc gtcaaagatc
     4501 gcctgggaca accggacgcc gaggaaacgg accatgggca ggtccggcac gatgaagcgg
     4561 ggcgtgaagg ccgggctcca gtaggcattg gtgagcgtgg cgtaggggat gctcagccgg
     4621 cgtgccgtga tggacagggt gatgcggaag tcgccgatca ccagatcggg tttttcggcg
     4681 ctgaggacgg cttcgtcctc ctcggcgtag cgtttgaggg tggcgtagtc gaagaccggc
     4741 cggcctttgg ccaggttgtc gatgaagcgg gccttgccgg tcggtgcgag ggttcgcgtg
     4801 cggccgggaa agccggcaag gaagcgggca gagtcggccg acgtggcgag caccacgtcg
     4861 tgcccgtcgg cgtggagcag ttcacccagc ttcagcggac gccccacatg ggccagggtg
     4921 acgtcatggg ccatcaggag gatcttcttg cgggacatga cggtggactc cgttgggcgg
     4981 cggcgggaat tgtcccggtg gtggtcggga atgtccagcc gaccgctgtt cagggcgcgc
     5041 gggccggatc cggccgcaag gtgacgaatt cgagctggca gtcggcgtgg aggcgttcgt
     5101 cgggtgcgtc ggtggtgtcg agacggctga cgcgacagcg gcggggcagg agcagggtgc
     5161 ccggctgtcg gtcgaggacg tcgagcagcc tgaggagctg gccttcgtgg gcaagggggc
     5221 cccgcaaggt cagcggcgag gcatcgaaat ccgccgcgcc gtcgagcggg cggtggggcc
     5281 ccagttcgac gtccagggag gcgaggtgct gggcttgcag ttccgcacgc acggccttga
     5341 gccaggcggg ccggtccggc ccggcggaca gcccctgtgc gcgcagttgg gcggcggcgg
     5401 cgagctgctt ctgtcgggct gcgagggtgt cttcggcgac ctggaggcgc gtgcggacgt
     5461 cggtgagcgc gtgctgcgtc tgcaggaggc gggcctccgc gtccgcgtgg cggctggccg
     5521 tgctgtaggc cgcgcccccg ccgaggaggg cgagggccag ggccgctgcc acgctgcggt
     5581 gggcggggcc gggccggatc aggggaaggt gactcatggc ggcggcgcgg ggctgaagcg
     5641 gaccacgacc cgcggttgtg cttccggcgc cggtcggagc cgaacgctcc gggtggtgtc
     5701 cagttcgtcg ggcacgcgtt cgaggctggc ctggacgtag ccggccgaga tcaccgcagc
     5761 gcggaggcgg tccagcctcg cggcaagcgc ggggggcgtg tcggccaggg ccgccggcag
     5821 ggcaatctcc gccaccgcca cgggggggcc gtcgggcggg agctgccact ccacggactt
     5881 cagttcgctg tcgggctcgg cctcaagcag gtcggacagc cggtcgagca gccttgtcgg
     5941 ccagttccgg gcgtcctgaa ggcggctcag gctttgcgtg gcggcgaggg ggtcgccggc
     6001 tgggggcgga ggcaacgcgg cgttcacgcg gctgaggcga tcttcggtcg cgacgacctc
     6061 ggccgtcagg cgggcgtcgg tgtcgctgag cgcccgggtg tcggccatga acttgatggc
     6121 gacggccgcg ccgatggcgg cggcggtggc gccggccagc aggatggcct ggtcgacggc
     6181 gcgcaggggg cgggcggtca gccggtctgg cgggacgaat tgggggagcc cgcggcggcc
     6241 tgcaccgtag aggatgtagg gaagggcgtc ggtgtgcggg ccggggggcg gcaggcggca
     6301 gcggcgggcg agctcggtgg ccgacggcgc gatggcctgc aggccggctc ccagcgcacc
     6361 cgcgaaggtc tccaggccgg gcagcagcac gaccgggagg ggatgttcgg ggtggagcag
     6421 ccgacgggcc acgagatggt cgcgggcggc attgcagatg ttcgggagat cggtggacaa
     6481 atcggtgact gcggtggctg gcaccaggcg gctccactgc ggatgtttgt cgacgagggc
     6541 cgtgaggcgc aaaccggtgt gcgtcagacc cgcaatggca caggtcgggg cgccggacgg
     6601 caggtggggc cgcagcgcgg ccattgcgtc ggcgacgctg tgcagaccat cgagccgggc
     6661 gccggcgtgg cgcagggcat ccagccaggg ctgcaatgcg gcggggcggg tgagtgcgtg
     6721 gaacagcagg gtttcccgcc cgtccgcgcg gccaagcggg cggatgccga caaagggcgt
     6781 cgaggcaaag agccggctgc gccgccgttc gatgagctgg tggcgctcct gaccccgcag
     6841 gatgggcagt gcgtcgatga gaaactgctc gtccggcaga tcggccatca cccgcagtgt
     6901 catgcgtggg tgggcgccga gccaggccga gaacgcggcg tcggcctgcc cgaaacgggc
     6961 gctggcccgg tatgcaccgg tcgtccggag ataggcggtg gcgccatccg cgcacaggtg
     7021 gaggaggcag agcgccatga tcagagcttc atgcgggtcg cgagatcgta gaccggtagc
     7081 agcacggcgc tcatgaccca caggaggagg ccgccgagga gcagggtgag ggcgggctcg
     7141 aggctggcct gcacgcgggc gatggcttcg cgcacgtccc gttcgtagaa atagcctacg
     7201 ttggcgaggg ccgtgtcgag ggcgccggtg tgctcgccga cacgcagcat gcgggcgacg
     7261 aggggcggaa aggcgggcgt gtcggcgaag gcgtccgaga cccgccggcc gtcaccgatg
     7321 gcgtcggcga tgcgctgcag ggccgcgcgc agggcggtgt tgccgaccac gccctcggcc
     7381 gcggcgaggg cgtccacgat ggtgatgccg gcgccgtaca tcatggcgaa gagggcggtg
     7441 aagcgggcga ggatgatctt gcgccggatc tcgccaagga ccggcagcgc cagttgggcg
     7501 ccgtcgtacc agcggcggaa gtcggggcgg gtggcgaggg cgaagcgcag ggcggcgacg
     7561 gcggcgagcg ccagcactgc cagcaggcgg ccgtgatcga tcaggaggtt cgacaggccg
     7621 atgagcaggc gtgtctgcag gggcaggggc tggccggtgg catggaacag gcgggcgagt
     7681 tccggcacga cgtaaagcag ggcgacgacg agggcggcgg cgatgacagt gccgacgatg
     7741 gcggggtaga tggcgatccg gcgcgcgaag gcggcgagtt cgtcgtcacg cttgagcccc
     7801 tcggcgaggg cgtgaaggac ggtgggcagt tgtccggcct gttcgccggc gcggaccagg
     7861 ctgacaaaga cgccgtcgaa gagcgcgggg tggcgttcga gggcgcggga caggctgagg
     7921 ccgccctgga tgccgtcgac aagggcgccg agggtgtcgc gcagcaccgg gtggggcgtg
     7981 ctgtcgcgca tgtcggcgag gctttcgagg atggggacgc ccgcgcgctg acactgctcc
     8041 aggtggaagc agaagttgat gagttcgcgc cgcggcacgc ctcggcgccc tccccggggc
     8101 gtccggctgg ccggggtggc gcggatcagg tcgaggccga ggcggcgcag gcgcaggtcc
     8161 agatcgtcca catggtgggc ttccagttcg ccttcgcgga gttggccgtg ggcgtccgcg
     8221 gcgcggtagc ggaagcgggt catgggcgca tgcggtcggt gaggtccacc acgcgggcga
     8281 gctcctcgag actggtgtgg ccttccagga cccgccggct gccgtcttcg gcgaggctcc
     8341 ggaagccctg gtcgcgggcc gcggcgagga gctcgcgtgg gctggcgcgt cgtgcgacga
     8401 gttcgtcgag gcggtggtcg atgcgcagca gttccatgat ggccatgcgc ccgcggtagc
     8461 cctggaactg gcattggggg cagccgcggg ggtggtggag ccgggggcgg ggggcgtcgg
     8521 cgggctgggc gagcaggtgc agttcgtagt cttctgccgg acgggcttcg cggcagtggg
     8581 ggcacaggcg gcgcagcagg cgctgggcga cgatgccgac gaggtggccg gccaggatgt
     8641 cggccgcgac gcccaggtca agcaggcgcg gcagggcgcc gacggcggag tgggtatgca
     8701 gcgtggagta cacctggtgg ccggtcatgg cggcctgcag ggccatgcgg gcggtgtcgg
     8761 tatcgcggat ctcgccgacc aggatcacgt ccggatcctg gcgcagcatg gcgcggatgc
     8821 cgttgccgaa atccaggtgg gcggcctcgg cgacggcggt ctggcggatc aggggcatgg
     8881 ggtactccac cggatcctcg agggtcatga tgtggatgcc ctcggcgttg atgtgattga
     8941 ggatggaata cagcgtggtg gtcttgccgc tgccggtggg gccggtgacg aggatgaggc
     9001 cctcgggacg ggcgatcatc agcttgaggg tgtcgagctg ggtgtcggcc aggccgagac
     9061 ggtcgagggg gacgatgccc ttctggcggt cgaggacgcg caggacgatg ttctcgccgt
     9121 gcagcgtggg gtgggtggag acgcggaaat cgacgggacg gccgttgacg tgcagggaga
     9181 tgcggccgtc ctggggcatg cgggtctcgg cgatgttgag gcccgccatg accttgatgc
     9241 ggacggccat ggcgggccag taggacgtgt gcagggcacg gatctggcgc agcaggccgt
     9301 cgatgcggta gcggatgcgc aggaagccgg gttcgggttc gaagtggatg tcggaggcgt
     9361 cggacttgac cgcgtccaca agcagcgcgt ccaccaggcg gacgaccggc tgggcctgct
     9421 ccaccgggcc cgccgcgccg gcctcgatct cgtggaggat gccgtcgatg gacagcgcgt
     9481 ggccgtagag ctggtcaatg gcccgggcga tttgcgattc ggccgccagc acgggctcga
     9541 tgtgcaggcc ggcggggagc tgggcctgca agctgtccag caggatcggg tcgccgatgc
     9601 gcgcagcggc cacggtgagc acccggctcg tgctgtccag gctcacgggg aggacgttca
     9661 ggcgacaggc caggtcgcgg ggcagcaggg tgagggcggc agggtcgggc accagggtgt
     9721 cgagatccac gctgcgccgg cccagggcat gggacagggc gtccctcagc gtggcttccg
     9781 acaggaatcc caggcggacc agcacgcgac cgatcgcttc gtggctgcgc tcctgctccc
     9841 gcagggcgat ggcgagttga tcggcactga tcaggccccg ttgcaggagc gcctggccga
     9901 ggggttgtcc gttcacggtg cgttgtccgg ggctgccagg gcgtcgatgc gtgcggcgca
     9961 ggcggcccgg tcgaatcccg ccaggtgccg ttcggccagt tgcagcgcga ggcggtaatg
    10021 cgctgcggcg ggaccgggct ggcgaaggtg atcgaggctg accgccaggt tgtaggcaat
    10081 gtccggatct tcgggcgcga ggctgcgggc acggaagaag gcgtgctggg cctcggccca
    10141 gcgctgctgg cgggccagca ccgtgccaag ggcgaactgg gcggtggcgt cgccggcgtc
    10201 cgcgtcgagg ctgctgcgca ggcggctttc ggcctggcgg ctgtcgttgc cgggcagact
    10261 ggcaagcccg gcgagggctg cgctgtccgt ggggcgggcg gcgagggcca ggcggaagtg
    10321 gcgggcggcc tcgacgtgca gaccgctgtg cagggcgagg gcgccaagcc cgtcatgggc
    10381 gtccgggttg gtgggatcga gggacagggc ggcttggtag tgctggtgcg ctgcggtgag
    10441 atcgccgcgt gtgtaggcgt cgtgggcgcg gagaaggtcg ggctgtggcg aggccaccgg
    10501 catcgggcgg ggctcgaagc gcagtgcggc gtgcgcgtcc gaatcgggcg ggtcggtgcg
    10561 ggcgggggag ggtggccgcg cggccggcga cgtggtgcga gaccgggcca cggcgggtgg
    10621 tgccgcaggc agggtttccg ggggtgtttc cggtggcggg gcggacacag ggggctgcac
    10681 ttcggtcgag gcttcggcgc cgcgtgcctg ggcaagctgg atgggggaca ggccggtcgg
    10741 gcgggtttcc gaccacaccc atccggcaag ccccgccagt ccggcgaccg tcaggccggc
    10801 gatccaggcc caccgggcgt gggagtgtgg cgtggtggcg ggtgtcgtgg gggccatccc
    10861 cgggggcggc gggtggatgg gctgaggtgc ggtcgtgaag ggcggcggcg cagggggctg
    10921 gctgttgcca gttggggggg cgttgagggg ttcgaggctc aggggcgagg ggcacatgac
    10981 agggctgcgg gcgtcagcgg cgggcgtctg gctggcctgc tccgtatgga tgtcgtccgc
    11041 tggggcgggg gcgggggcgg gtggcggcgc ggaggcgccg ggcggtgtcg tttcgccgcg
    11101 ccgttcggcc tcgtgcttgc gcaggacttc caggagcagg ctcatggggt ggtgtcagga
    11161 aagttgcggg tgccgggtag cggaccgcgg cggaagaagt cgtcgcgggg gaggctgttg
    11221 cgcagggggc tggcggccag gagattgccg tcgcggacca ccaccgggcg caggaagatg
    11281 accagctcgg tcttctggac caggttgtcg cgggtggtga agacctcacc cagcagcggc
    11341 acggcgccga cgccggggac gcggccggtc ttgaagtcga cccggtcttc catgagcccg
    11401 ccgaggacgg cggtttcgcc gttttcgata cgcagcacgg attcgatctc gcgggtgcgg
    11461 atctggggga cgaggttggg aatcgtggcg ggaatgttgg ggttggggtc gcggcggaag
    11521 tcggcgatgc tggaaatggt ggggcgcacg ttgaggatga tctcgtcagc ctcgctgacc
    11581 tggggcgtga cggtcatgac gaggcccact gagacggcct ggggcgtggt ggtgacggtg
    11641 gtgagggcgc ccgcgttggt ggtctgggtg gtgtcggcct tgacgttgaa atagacgtat
    11701 tcctcgacca ccttgatcac cgccgtctgg ttgttgagga ccgacagttt ggggctggag
    11761 aggaccttca cggtgccgaa gccttcgagc aggcggatcg tggagtcgat gccgtcggcg
    11821 ctcttgtagg cgacgctgaa gggattgacg gcattgccgg cgctgctgcc gagggtcggt
    11881 ccggtgatgc cgaaacgggt gccgctcgag agcacgcggt tccagtcgat gccctgacga
    11941 taggtgtcgg acaggccgac ttcgaggatg gtggcttcga tgagcacctg acggcgggcg
    12001 gccgccacca cctgatcgag gaattcctgc accttttcgt gctggcggcc ggtggcgcgc
    12061 acagtgagga cgccggtttc ccggttgacg atgacggaag cggcttcgcg gaaggtggcc
    12121 ttgtggacca ccgtggtggc gctgttctgt aacagggcgg gattgggact cgccgcgatg
    12181 ccggcttgcg gtgacgcgga ggtggtgcgc gtccggctcc gggcggccgt ggcggagggc
    12241 tgtacgccgg tgccgctggt gctggtggtc tcgaggtttt cgatgacggt gtcgctggag
    12301 ccttcgggga ggagcttgtc ggtttcccgc agcaggtcgc ggatgttgcg ctcgatggat
    12361 tcccagaact ggttgcggga ggcgttttcg atgcgggtgg ccgaaacgtt gccgccgttg
    12421 gggagggcgg ccgtctggag tgtggtgctg ccgctgacgg ccgagccgct gccgctggtg
    12481 gcgatctggg tgttggtgga gaccgtgccg gtgacgctgc ggctgagatt gacgtaatcg
    12541 acgcggtacg tgcgcaggta ggggctgtcg ggcatcaccg ccaggtgggt gccgtcgagt
    12601 tcgaagcgca ggtcggcctg tcgggcgagg cgcgtcagga tctggggcag ggtttggtcg
    12661 atggcgttga gggtgaccgt gccgttcagg cccgggtgga tgtccacgtt caggcgggcg
    12721 tcgcgggcga gcgcgaacag gagatcctgg agccgcacat tgtgcaccac gacggagaat
    12781 gtctcggggc gcggcgtggc ttgcgggcgg ggcaggatca ggctcggccc ggcaaggggc
    12841 ggaatcgttc ctgcgggaaa tgccgcattc ctgtcctgga tatggctttc ggatggcgcg
    12901 ggggctcggg tggacagcat tggcgcacag gcgctggtgc tcaggagcag gattgcgcag
    12961 gcagagagac gtatacgcat caacaggaat ccggggtgta tgcgtataaa atatgacagt
    13021 ttccgggctt tgtcgatgct ttctgaattc ccggtcgatg actgaaaatt gctgcgttgc
    13081 cgcaataatt gcggcgcgac gtggcctccg ggcggcgcgg tgacggcgtg tcgggtggcg
    13141 gtctgcggaa atgaaaaaat ctcgtggaga gggtgtaagt cctgtgtaag attaagcgcc
    13201 tttaatctcg ggcggactcg atcggaaata cgcagtacaa gaaaaacaga actcaatccg
    13261 gtcagtggac ggcgcgtctg gtcctgcatc cgcccaacaa ggtttcaaat ccactacgtt
    13321 gcacactcac aacacgagga gaggtgaaaa atgacgagcg aaaccaccca gaaactttac
    13381 tatccgtctg aagaggtggt caaaaacgct gccgtgtcgg gcatggaagc gtacaaggcg
    13441 ctgtgcgccg aagcggaagc cgattacgaa ggctactggg gcaagcgcgc caaggaactg
    13501 atcgaatggc agaccccttt cacccaggtg ctggatgaga gcaatacgcc gttcttcaag
    13561 tggtttgccg atggcaagct gaacgtctcc tacaactgcc tggatcgcca ggtcaagaat
    13621 ggcctgggcg acaaggtcgc catgatcttc gaaggcgaca agggcgatgt caccaaggtg
    13681 acgtatggcg atctgctgac ccgcgtcagc aagttcgcca atgcgctgaa gagcctgggc
    13741 gtcaagaagg gtgaccgggt cgtcatctat ctgcccatgt ccatcgaagg cgtggtcgcc
    13801 atgcaggcct gtgcccgtat cggtgcgacg cactccatcg tgttcggcgg cttctccgcc
    13861 cagtccctgg ccgaccgcgt caatgacgcc ggcgccgtgg tgctgatcac ctccgacggc
    13921 cagaaccgcg gtggcaaggc gctgcccctc aaggccatcg ccgacgaagc cctcgccagc
    13981 ggctgcccgt ccatccagaa ggtgctggtg gtcaagcgca cgggcggcga aactgccatg
    14041 gtcgaaggtc gtgacgtgtg ggccgacgac atcgtcgccc agcagtccga tgtctgcgaa
    14101 ccggaatggg tcgaggccga gcatcccctg ttcctgctct acacctccgg ctccaccggc
    14161 aagccgaagg gtgtccagca ctccaccggc ggctacctgc tgcacgccat cctgaccatg
    14221 aagtacacgt tcgacatcaa gccgaacgac atcttctggt gtaccgccga catcggctgg
    14281 gtcaccggtc atacctacat cacttacggc cccctggcct gcggtgccac cgagatcgtc
    14341 tttgaaggtg tgccgactta cccggatgcc ggccgtttct ggaagatgat ccaggaccac
    14401 aaggtctccg tgttctacac cgccccgacc gcgatccgtt ccctgatcaa ggcctccgac
    14461 accaccccgg cggtgcaccc gaagaactac gatctcacct cgctgcgcat cctgggttcc
    14521 gtgggcgagc ccatcaaccc cgccgcgtgg gagtggtatt acgagaacgt tggcggcagc
    14581 cgctgcccga tcgtcgatac cttctggcag accgagaccg gcggccacat gatcactccg
    14641 atgcccggcg ccacgccgct ggttcccggc tcctgcacgc tgcccttccc gggcatccag
    14701 gcggcggtgg tcgatgagac cggcaccgac gtgccctggg gccagggtgg catcctggtc
    14761 gtgaagcgtc cgtggccgtc catgatccgc accatctggg gcgatccgga gcggttcgtg
    14821 aagtcctact acccggccga cttccagggc aagctgtacc tggcgggcga cggtgcgatc
    14881 cgcgacaagg acaccggcta cttcaccatc accggccgta tcgatgacgt gctgaacgtc
    14941 tccggtcacc gtatgggcac gatggaaatc gaatccgccc tggtggccca cgagaaggtg
    15001 gccgaagccg ccgtggttgg tcgtccggac gacctgaccg gcgaagccat cgtggccttc
    15061 gtggtgctca agggtgcccg cccgtccggc gacgaagccg ccaaggtggt caaggagctg
    15121 cagaactggg tgggtcagga aatcggcccg atcgccaagc ccaaggacat ccgcttcggt
    15181 gaaaacctgc cgaagacccg ttccggcaag atcatgcgcc gcctgctccg tcagctggcc
    15241 aagggcgagg agatcaccca ggacacctcg acgctggaaa acccgcatat tctggatcag
    15301 ctcaagtaag cattgtgtgc cggccggtca ggccgggcac gtcctgctac cggcgattca
    15361 ccccgagtcg ccggcataaa aatccagtgc gggacgtgag aggaacgatg gggaggaacc
    15421 cggcctaatg agccgggcaa gagggggaga aaagggcgcg aatttcgcgc ctttttctcg
    15481 tctacgccgg gaagtgggtc ctccgcccgg gcgatgccga aggttctcgc caagcccgcc
    15541 gattcggttt ataaatctgg caccggcgga cgcgctcgat gccggacata aaaagaaaac
    15601 ggaggagcag ggatgatcaa ggcgaccctg atggggcagc ggctggtcgc ggtattcgtg
    15661 ctgggatgtg tgctggccaa ttttccggtg ctgtcgatct tcaatgacga tggcgcctgg
    15721 cagggaatcc cgctgctcta tctttatgtg ttcgggctgt gggcggcggt aatcgtgctg
    15781 atggcgtgga tcgtcgagcg atgatgcaca cctgccgcat tcccgtacct gaaagaggat
    15841 aggcgatgct gtccggcaca accgttgtcg cggtgtcctt cctttatctg gcggccctgt
    15901 tcggagttgc ctattacggt gacaagcggg cagatgccgg gcgctcggtg atcgccaacc
    15961 ccacgatcta cgcgctgtcc ctggcggtgt attgcacaac ctggacgtat tacggttcgg
    16021 ttggccgggc ggcatcgtcg gggataggtt tcgtgccggt ttttctcggg ccgacgctgg
    16081 tggtgggatt gtggtggttc gtgatgctga agatgatccg catcggcaag tcgaaccgga
    16141 tcacctccat cgcggacttc atcgcatcgc gttacggcaa gagccatctg ctcggcgggc
    16201 tggtcacggt gatcgcggtg ctgggcgtga ttccgtatat cgcgctgcag ctgaaggcgg
    16261 tctccggcag tttttcgatc ctcatccatc ctcagggcct tctgacgccg atccggaccg
    16321 gcgccctgcc atggatgacg gacaccacgt tctatgtcgc gctgatgatg gccgcattca
    16381 cgatcctgtt cggcacccgt catctcgatg ccacggagcg ccacgagggg ctggtggcag
    16441 cggtcgcgtt cgagtcgctg gtgaagctgg tgatcttcct ggtagtgggt ggcttcgtca
    16501 cgttcggact gttcgacggt ttcggcgatc tgttttccca ggctgccgag gatcccgccc
    16561 tccgggcatt gatggtcgtc gaaacgggcg gcaacagtta cggaaactgg tttgcgctga
    16621 ccgtgctgtc gatgctggcg gtgatgttcc tgccgcgtca gttccaggtc gcggtggtcg
    16681 agaacgtcaa cgagaagcac ctgcgtcggg cgatctggct gttccccctg tacatgatgc
    16741 tgatcaacgt gttcgtgttg cccatcgccc tcggcggact gatttacttc cgtgggcagg
    16801 cggtggatgc ggacaccttc gtcctgaccc tgccgctggc atcgggtcat cccctgctgg
    16861 cgctgctggt cttcatcggg ggattgtcgg cggcgaccgg gatggtgatc gtggaaacga
    16921 tcgccctgtc cacgatggtc tgcaatgatc tggtgatgcc ggcgctgctc cgattcagcc
    16981 ggggggcgct gcgcgggcac aacgatcttt ctggcctcct gctggcgatc cggcggggcg
    17041 ccatcgtgtt gatcctgctg ctggcctacc tgtatttcag gttggcgggc gaggcgcatg
    17101 cgctggtgtc gatcggtctc atcagctttg ccgcggtcgc ccagtttgcg ccggccatgc
    17161 tgggtgggat gtactggcgc ggagggtccc gggcgggggc gctggcggga ctcagtgccg
    17221 gcttcctcgt atggggctac acgctcctgc tgccgtcatt cgccaaatca ggctggctgc
    17281 ccggcgattt cctgcagggg gggctgttcg gactcgagtt cctgcgaccg caggaactgt
    17341 ttggtctgcg cggactggat cccatggccc acagcttgtt ctggagcctg tttgcgaatg
    17401 tgggcgcgta tacgtgggtg tccctgttga ggccgcccaa cgcggtggaa gccgggcagg
    17461 cgaccttgtt cgtggatgtc ttccgccagg gacggcccag cgggggggct ttctggcgcg
    17521 gcagcgcgcg ggtcggcgac ctgcttcccc tgattgcccg tttcgtggga ctggagcgcg
    17581 cccaggccct gttctcctcc tacgcccgcg agcgcggcgt cgagcgcgtt gcagacctgg
    17641 aggcggacgc cggcctggtt cactttgcgg aaacgaccct ggcgggtgcc atcggcggtg
    17701 cgtcggctcg cgtgatggtg gcgtcggccg tcaaggaaga gccgctcggg ctggacgagg
    17761 tgatgcacat tctggacgag gcctcccagg tccgtgccta ttcccagcgc cttgagcaga
    17821 aatcccggga gctggaagcc gccacggcag agttgcgcca tgccaacgac cgcctcaagg
    17881 aactcgaccg cctcaaggac gatttcatgt cgtccgtgac ccatgagctg cgcacgcccc
    17941 tcacctcgat ccgtgccttt tccgagatgc tgctggaaga cccgcgcatc gatctggccg
    18001 atcgcaaacg ctttctgggc atcatcgtgg ccgagaccga acggctgacc cgcctcgtca
    18061 atcaggtgct cgacatggcc aagatcgagt ccggtcacgc ggaatggcac aacggcgagg
    18121 tgaatctggt ggaggtggtg gaacaggccg tggcgtccac aagccagttg ttcaaggacc
    18181 ggggtgtgag cctcgagctc gatctgcccg cgtcgctgcc ggtcctgctg gcggaccggg
    18241 atcggctcat tcaggtgatg ctcaacctct tgtccaacgc cgtgaagttc gtggagcggg
    18301 acggcggtca ggtccgcgtg aagctggcgg aaacgtcgga cggcgtgcag gtggacgtca
    18361 gcgacaatgg ccccgggctg acgttggccg aacagacggt cgtctttgaa aaattccgtc
    18421 aggggggaaa cacgatgacc gccaagccgc agggcaccgg tctcggcctg ccgatcagcc
    18481 ggcagatcgt ggagcattat ggcgggcgtc tgtgggtgga gagtgctccg ggtgagggcg
    18541 cgcggttctc ctttttcttg ccttgcggca gatcaacaga agcgcaagag agagcgggat
    18601 aaactataca atcgagccaa ccggggcaga ggttcgcgtg tgcgcggatc ggacctgcgg
    18661 agacataagg aggggggact gaatgaccaa aaaaatactg atcgccgatg acgagcaaaa
    18721 catcgtcatc tctctggaat tcctgatgaa acgcgagggt tttgccgtct gtatcgccaa
    18781 cgacggtcag gaggccctcg acaaggtacg cagcgaatcg cccgatctgg tgctgctgga
    18841 cgtgatgatg ccgcgcaaga atggtttcga agtctgtcag gagatccgtg ccgacggcgc
    18901 gctgcaaggc gtgcgcattc tgatgctcac tgccaagggc cgtgagaccg aagtgagcaa
    18961 ggggctggcc atgggggccg acgcctatat gaccaagccg ttctctacca aggaactggt
    19021 ggcgcgggtc aaatccctgc tggaattgcc cgcgtgagcc ccaaggcccg gcaggcgtgg
    19081 ccgattctgg ccgcctgcgg gatggtggct gccatcctca tttctgcggc cgtggccctg
    19141 tggcgcgatc tgggcgtcga tgaacaggcc gttctggagc ccatcctcaa tccccggctc
    19201 ggtctgctga tcatgatcgg tctggcgggc tgcggtgccg tcgccgtggc gatgaagtcc
    19261 ttcgtccagg cccatgtggc tgcgcctgca ttgctcgccg acgagatcgg cctgatcgtc
    19321 gggacagatc cggcgcggcg cctcaaggcg caggccgatc ccgacctgaa cagcattgcg
    19381 gatgccgtga atgcgctcgc ggaccagcgc cgcagtcttc aggacgacgt ggaagcgcgc
    19441 attcgcgaag cccgcctgtc cgtggaggag gagcgcaacc gcctcgccgc actgatgtcg
    19501 gaactgaccc agagcgtcgt ggtgtgcaac ctcgatggcc gtgtcctgct ctacaacaac
    19561 cgcgcccggc tgcagttcaa ggccttgtca gatgcgcccg cggcggccgg tggcggcgag
    19621 atcatcggcc tggggcgttc catctatgcg gtcttcgagc gcaatctcat cgcccatgcg
    19681 ctggagagca tccaggaacg tctgcggcgt cagagcgccc agccggtggc caacttcgtc
    19741 acgacgaccc gcgccgggca gctcttgcgc gtgcagatgg cgccggtgct gtcggtgggt
    19801 gagggcagcg cggagccggc ggtgacgggc tttgtgctga tgctcgacaa catcacccgg
    19861 aatttcgagt ccgagacgcg acgggatcag gtcctgcaca ccttgaccga aggcagtcgc
    19921 tcggcgctcg ccaacgtgcg cgctgccgtg gagatgctgg aatttcccga tctcgatgcc
    19981 gatctgcgcg aacgtttcct gacggtcatc cgcgacgaag tgcgggggat gagtgaacgt
    20041 ctggacaaga ccgccagcga gtttgcggac tcactcaaga cccgctggcc gctggatgaa
    20101 atgctgggcg cagacctggt ggcggcagct caacgccgca tcgagaaaaa gatcggcctg
    20161 ccgaccaagc tggaagatgt ggacgacagc gtgtgggtca aggtggacag cttctccctg
    20221 ctgcaggccg ttggctacct ggcaagccgt ctgtccgacg agttcgaggt gcgcgaggtg
    20281 cgtctgcgcc tgacctcggc cgggcggctg gtgcatctgg atctgatctg gtccggtcag
    20341 gccatgagca cggagacggt gatggcctgg gaactcgagc ccatgagctt tgccggcgag
    20401 agttcgccgc tcacggtgcg cgatgtcatc gaacgtcatg atggcgagat ctggctgcaa
    20461 cgggaaaaga ctcgtcatcg cgccttcttc cgcgtgctga tcccgtcggc cgcgccccag
    20521 gagcaggtgg agtcgtccgc ctatctgaaa ggtgacagcc ggccggaata ttacgatttc
    20581 gacctcttca aacattccga agcgagcgat gccctcgacg accgcctgct ctccgagctg
    20641 acctttacag tcttcgatac ggaaacgacc ggtctcaacc cctccgaggg cgatgagatc
    20701 atccagatcg gtgccacgcg catcgtcaat ggcaagctgc tcaagtccga atccttcgag
    20761 caactggtgg atcccttgcg cgacctgccg gaggcctcca ccaagatcca cggcatcacg
    20821 cccgagatgc tcgaagggca gcccgccctc gacaaggtat tgccggcatt ccacgcgttt
    20881 tcccaggata cggtgctggt ggcccacaac gcggcgttcg acatgcgttt cctgcagatc
    20941 aaggaagaga gcctgggcgt gcgcttcacc cagccggtgc tggatacgct actgttgtcg
    21001 gcggtgatcc acccggccca ggagtcccac cgtctggagg ccatctgcga gcgcatgggg
    21061 gtcaatgtga tggggcgtca cacggccatg ggggatgcca tcgtcacggg agaggtattc
    21121 ctgaagatga tcccgctgct ggccgaggcg ggcatccgga cgctgggcga cgctcgccgt
    21181 gcgtcggaga agacctatta cgcacgggtg aagtactgag cggttgatga gggcgcaggt
    21241 gctctccgac cgcaatccgg tgatgttgcc gccggccagg aggggcgcat gagttcgtcg
    21301 cgggaatccc gtcctttatc tggcgatggt caggcgcttg cgtccctgct gcggcgcgct
    21361 cctgtgtcgg tgccgcccga atcgcctgtg cgtgacgtgc tcaagcggat gctggccgcc
    21421 cgcgtcggtt cggtggtggt ggccgacgtg gacacgggct tcccgctggg cgttctgacg
    21481 catcgcgatg tgctggagcg ggtggtgctc gcagcaggcg atctggacgt gccggttgcc
    21541 agcgtgatga ccggcggcgt aatgagtctg ccgctcggtg cgaatgtcca cctggctcgt
    21601 ctgctgctgg cgcagcgtcg tctgcgccgg gtcgtggtgg tcgatgcgga cggccgtctg
    21661 gccggagtgg tgtcgcgcgg ggatctgtat gcccagcggc agccgtgcgc cgacgatctg
    21721 gtgagccgga tccgggctgc cgaccatgtc gaggcgctgc gcgacgctgc ccgtggtgtc
    21781 cgcgatgcag cggccagatg gctggatgac ggcgccgggg ccggtccggt tgccgaatgg
    21841 attgcccttc tcaatgatct cgtggcgcag gcggccctgg acctggctct acgggatgtc
    21901 gaactgccgc tggtgcgatg gtgctggctg gcgttcggct cagaaggccg tctggaacag
    21961 accttcgaga cggatcagga caacggcctg atcttcgaag tgccggacga tgtggatgct
    22021 gatgccatgc gggagcgttt cctgccctgg gcgcaacggg tcaatgatgt gctcgacact
    22081 tgcggattcg cccgctgtgc gggggggatc atggccggca acccgacctg gtgtctgtcg
    22141 gtgggggaat ggcgggagcg cttcctgagc tggatgttgt cggcggaccc cgaggcgctg
    22201 ctgaatgcaa ccatcttttt cgactttcgc ccgctggcag gggattggga gttggccgga
    22261 cgcttgcagg aatggcttct ggcgcggacg gcggagagcg agctgttcct gcggcacatg
    22321 gcggggaatg cgctgcgggg cggtccgccc ctcgggcgga tccgcgactt cgcggtggat
    22381 cggaagaccg gccggctgga tctcaagcgc gacggcagcc ggccctttgt ggacgtggcg
    22441 cgcatctatg ccctggccct ggggttgcgg gagacggcca cggccgaccg actgcggggc
    22501 gcggggcgct tgctgaactg gtcccgtcgc gagatcgatg ccgtcgtgga agccttccac
    22561 tgcatccagc gactgcgtct ggatcggcag cagcgcggcc agggaccggc aaacctgatt
    22621 gcacccgaag agttgaacga gctggagcgg gccacgctca aggaggcctt ccgtcaggcc
    22681 cgcaagctgc aggcgcggct tcagatccgc tatcagttgt aagtcgagag cccggctata
    22741 ctctgcccag tgccgatcgg gccaaggagg aatgtgcgtg gcggtagaca gtgcagggcg
    22801 tcgcgcctac tggcgggcca atctggcttt gactgccgta ttgcttgtga tctgggccgc
    22861 ggtcagtttt gtgccgggct ggtatgcgga agatctggag gcctattccg tgttcggctg
    22921 gccgctcgat ttctacatgg cggcccaggg gtcactgatc gtatttctcg tcatcgtgtg
    22981 gctgtacgac cggtgcatgc gtcgtctcga gcgtcgcctc ggggtgcccg acgagcgcga
    23041 ctgaagcgtc tgccatgaag tcatcgcaca gctatttcgt tcgcctgggt cgttattacg
    23101 gctggtattc ggcggggttc ttcgtgctga tcggactgct cgcgatcgcc gagcagctcg
    23161 gaatgacccc gcgctggatc gggcagatct tcctgctggc caccattgcc ctctatgcgg
    23221 cgatcggcgt gatgagccgc acgtccgacg tttccgaata ttacgtggcc gggcgccgcg
    23281 tgcctgcggt gttcaatggc atggctacgg cggcggactg gatgagtgcc gcgtcattca
    23341 tcgggctggc cggcaccctg tattacaccg gcttcgaagg actggccttc gtcaccggct
    23401 ggacgggggg gtacgtgctg gtggcgctgc tgctcgcgcc gtacctgcgc aagttcgggc
    23461 aattcaccgt gccggatttt ctcggcgccc gctatggcgg ccatgcggca cgcattgtgg
    23521 gtgtggtggc tgcggtgctg gcgagctttg tgtacgtggt ggcgcagatt tacggcgtag
    23581 gtctcatcac cagccgcttc atcgggatcg agttcgagat cggtgtcttt gtgggcctgg
    23641 ccggcattct ggtgtgctcc ttcctggggg gcatgcgggc ggtgacgtgg acccaggtgg
    23701 cccagtacct cattctgatc gtcgcctatc tggtgccggt gaccatcctg tcctacaagg
    23761 tgacgggggt tcccctgcca cagctgacct acggcggcgt cctgaaggac ctcggcaaca
    23821 aggagctgga gctgttctcc gagccgcggg agaatgaagt ccgcacccag taccgctacc
    23881 gggcggatga ctacgcccgg aagatcatcg atctgcccga ttccctcgcc gccgagcgga
    23941 cggggcagct gcaacgggtc gaggaactgc gtactcaggg cgcctccgcc cgcgaactgg
    24001 ccgcagcgga gcgggccttg cgcgacatgc cgcgtacgcc ggatgaggct cgggtgaagt
    24061 gggaacgggc acgtcaggaa agtctggtcc gcgcggctcc gccggtgccg cacgcccagg
    24121 cccacccagg tcccgaccgg gcggcgcgcg acagcgcccg caacaacttc atcgcgctgg
    24181 cttttgtgct catggtcggg acggccgcat tgcctcacat cctgatgcgc tactacacaa
    24241 cgccctcggt gcggcaggcg cgcaactccg tgttctggtc cctgttcttc atctttttgc
    24301 tgtacgtgac cgctccagcc tacgcggtat tcgccaaatg ggaggtgtat agcggtctcg
    24361 tcggtacgcc catcgcgtcc ttgccggcgt gggtggcggc gtggggccgg gtcggtctgg
    24421 tgaagatcga cgacatcaac ggtgacggca tcctgcaact ggccgaactc tccctcaaca
    24481 gcgacgcgat cctgctgctg gtgccggaaa ttgccggcct gccctacgtg gtctccggtc
    24541 tggtggcggc aggcgggctg gctgcggcgt tatccacggc ggacggcctg ctcctgacca
    24601 tctcccacgc cctgtcccac gacctgtatt acaagatgat caatcccgag gcctcgacgc
    24661 agcgtcgcct catcatcacc aagtcacaat tgctgatcgt cgcggtgtgc gcggcctggg
    24721 tcgcctccct gcgaccggat ggcatcctgt tcatggtggg cctggccttt tcgattgccg
    24781 cgtcggcctt ctttccggcg ctggtgctgg gcatcttctg gaagcgtgcc aatcgctggg
    24841 gggccgtctc cggcatggca gcggggctcg gcatcacctt gtattacatc gtgcgcaccc
    24901 atccgttctt tggcggcagc ctgtccgagg cctggttcga tattcatccg atctcctgcg
    24961 ggatctttgg cgtgccggta ggattcgtga ccatcgtggt ggtcagtctg ctgaccccgc
    25021 cgcccgacgc cgcaacgcag cgcctcgtgg atttcatccg catgcccgac ggcatcgagg
    25081 attttccgga tcgcgacgaa aaggtgtttg acgcgggcgg aagttaatgt atgatcccgc
    25141 ctccacttgg agaggtggat gagtggttta agtcgcacgc ctggaaagcg tgttcagggt
    25201 aataccctga cgcgggttcg aatcccgccc tctccgccaa gaatttaccc ggatgttcgc
    25261 ggcgtgcgca tgcaagtgcg cacgctttcc gtttataggc ggccgcatcc gggggtgttt
    25321 caccttgccc gaccgccccg catggtcggc gggctattat ccataaggag atcccatgcg
    25381 gcattacgaa atcgtcttca tcgtgcatcc tgatcagagc gagcaggtgc ccgcgatgat
    25441 cgaacgttac acgacgctcg tgaccagtca gggtggtcag gtgcatcgtc tggaagactg
    25501 gggccgtcgt cagctggcct acccgatcca gaagatccac aaggcccact atgttctgat
    25561 gaacatcgag tgcgaccagc ccaccatcgc cgaactggag catggcttca agttcaacga
    25621 cgccgtgctg cgtcatctct ccatccgcat gaagcgcgcc gtgactgccc cgtcgcccat
    25681 gatgaaggaa gagaaggccc gctccctgac gcccgcggcc gacgaagcca agccggccga
    25741 gcagcccgcc gcctgatttt gcaggaagca gggcgcaaca gtcttgagct ggaaggtgtc
    25801 ctgaccgaaa tcggtgcgct gcgtcgtacg cctgcgggga tccccgtgct ggcatgtcgc
    25861 atcgaacacc ggtcgaggca gatggaagca gggcggctcc gcgatgtcgg ggtcgaattg
    25921 caggcggtcg tggtagggga caagacgtcc ctcctggctg cggcgcccct gggaagcctg
    25981 ctgcacgtca ccggcttcct cgcggcgaaa agtctgcgta gcagggcacc gatactgcat
    26041 atcgatacga tcgaattttt ggaaggaaac agacatggca ttcaagcccg caatgaagcg
    26101 caaggatgac cgcggtggcc gcggcctctt caagcgtcgt aagttctgcc gcttcagcgc
    26161 agagaagatc gaggaaatcg actacaagga tgtggacatc ctgaaggatt tcatcaccga
    26221 aaacgccaag atcatgccgg cccgcattac cgggaccaag tccggttacc agcgtcagct
    26281 gtccaccgcg atcaagcgtg cgcgcttcct ggcgctgctg ccctacaccg atctgcacca
    26341 gtaatccggg agaacaagca tgcaaatcat tctgctggac aaggttgtga acctcggtgg
    26401 tcttggtgat gtggtcaagg tcaaggatgg ttacgcgcgt aactacctga tcccccaggg
    26461 caaggccaag cgtgccacgg ccaccaacct ggctgaattc gaggcccgtc gcgccgaact
    26521 ggagcgccag gcggccgagc aattcgctgc cgcccaggcc gaaggcgcca agctggaagg
    26581 tcaatccgtc accatcgccc gcaaggcggg tgtggatggc cgtctgttcg gttccgtgac
    26641 caatgccgac atcgccgacg cgctgaaggc gcagggcttt gccgtcgaga agtctgctgt
    26701 gcggatgccg gaaggcccgc tgaagaccgt ctgtgaagcc cagatcgtcg tgtccctgca
    26761 tactgacgtg acggccacga tcaccgtgac ggtggtcggc gagaactgat cggttcgggc
    26821 ttcggctcgc tgctgtggaa aagggctgcc cggtgcagcc cttttttctt gggtgcgcgg
    26881 ccgagggtcg cggccccggt ttattgtggt gacttccatg cgcaacgctt ccctttccga
    26941 agatcccgcc ctcgcttcgc ttcgactgcc accccattcg cttgaggctg agcagtcgtt
    27001 gctgggcggg ctgctcatcg acaatacggt gtgggatcgg gtgggcgaca tcgtcagtga
    27061 ggtcgacttc taccgggacg atcatcgtcg catcttccgg cagatcgcca aactcatcga
    27121 actcggcaag ccggcggacg tggtgacggt ctacgaggcc ctcgaaaagg ccggcgaggc
    27181 cgagcacgtc ggcgggcttg cgtatctggg agagattgcc aacagcacgc cttcagctgc
    27241 caacgtgcgc cgttatggcg agatcgtccg cgagcgcgcg atcctgcgca agctggttgc
    27301 ggtcggtgat cagatcgcgg ccagtgcgct ggcgccatct ggcaaggatg cgaagacgct
    27361 gctcgacgag gctgaggcca aggtcttcga gatcgccgaa gccggcgcgc ggaccacgac
    27421 cggcttcgtt cccatccagc cgctgctcgg gcaggtcgtg gatcgcatcc aggaactcta
    27481 cgaccgggat tccccgagcg gaatcactgg cgtcgccact gggttgaccg atctggatga
    27541 aaagacctcc ggcctgcagc cctcagacat gatcgtcgtc gcggggcgcc ctggtatggg
    27601 caagacctcg ttcgccctca atatcgcaga gaacgtggcg gttgaatgtg ggctgccggt
    27661 ggccgtgttt tccatggaaa tgccaggtac ccagctggcg acgcgttttc tgtcttcggt
    27721 cgggcgtctg gaccagcatc gggtgcggac gggcaagctc accgatgagg aatggcagcg
    27781 tctcaccttc gccttgggta agctccatga ggcgcccatc tacattgacg agacgccggg
    27841 gctgaacccg acggacctgc gcgcccgctg ccgacgcctg caccggcagt gcggacgctt
    27901 ggggctgatc gtcatcgact atctgcagct gatgacgtcg ctcaaggagt ccgacaaccg
    27961 ggccgcagag ctgtcggaga tttcccgctc cgtaaaatcc ctggcaaagg agttgcacgt
    28021 ccccatcatt gccctgtccc agctcaaccg gagcctggag cagcgcccca acaagcgtcc
    28081 ggtcatgtcg gacctgcggg agtccggtgc catcgagcag gacgcggaca tcatcatgtt
    28141 catctaccgg gatgaaatct ataacccgga ctcccaggac aagggcactg ctgaactgat
    28201 cgtggccaag caccggaacg gctccacggg caccatccgc atgaccttca tcggcgagta
    28261 cacgcgtttc gagaactacg tgggcggcgc cgccgtggcg gagtattgat tgctacacgc
    28321 gggtgaaatt tatttttcac taacgtgttg acggggcccg atagttcttt ataatgcgca
    28381 cctcttcttc acgcggtgca gcaaaggcgg cgcgg
//
LOCUS       DAOQMM010000042        27409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_519_length_27409_cov_45.369160, whole genome shotgun sequence.
ACCESSION   DAOQMM010000042 DAOQMM010000000
VERSION     DAOQMM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 27409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27409
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_27409_cov_45.369160"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLH85_08830"
     CDS             complement(<1..714)
                     /locus_tag="PLH85_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB64706.1"
                     /translation="MSTPVAARHYPPGGDAHGRAVTLKAAAGQLVVAPVEGAALHVPA
                     ARLQASPRGFNHSQWALAWAGADGDHLLIVDDAAVGPLRNGLPGLFATGDQARQRAGL
                     HRVFAYGVLILACLALVVGGVWGFDGLVDRVVAHIPASVEAQIGEAVLARTRQDGPFI
                     ETGAAAEAVQAIGRRLTRPGETLQFHLVQSPQVNAFAAPGGVVVVYSALLRQAGSADE
                     VAGVLAHEIAHVELRHSLRQ"
     gene            complement(813..1514)
                     /locus_tag="PLH85_08835"
                     /pseudo
     CDS             complement(813..1514)
                     /locus_tag="PLH85_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
     gene            complement(1653..2327)
                     /locus_tag="PLH85_08840"
     CDS             complement(1653..2327)
                     /locus_tag="PLH85_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HNB64707.1"
                     /translation="MKALHRSAPVRAVTARPRLIFSTICGVLALVALPDTWAHQPVPR
                     GILAWNIGCWLYLALSLTMMFRSTHERMQRRALDQDAGQRTVLALVLLAAVMSLGAIV
                     AELAVAKDLTGPSRLLHIAFAAVTILSSWLLTQVMFAQHYAHDFYLAEARQGSGGLAF
                     PGGHAPDYGDFLYFACVIGTSGQTADVSFTTRAMRRTGLFHCVLSYFFNATVLALTIN
                     IASGLV"
     gene            complement(2413..2646)
                     /locus_tag="PLH85_08845"
     CDS             complement(2413..2646)
                     /locus_tag="PLH85_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64708.1"
                     /translation="MRLNLSLSILALAVVAGCSSPSHASTHERALVTAELVASRPACA
                     GFIRQLRETVATDQALAETYAAMRAAGCLRPDV"
     gene            complement(2894..3331)
                     /locus_tag="PLH85_08850"
     CDS             complement(2894..3331)
                     /locus_tag="PLH85_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64709.1"
                     /translation="MYDILTLEPEDDVVMRAIKAVIAGRATIGGPEGDYRLLTPGDTW
                     VEGDGGTGVEVLVIVGHGNANKLSGCGKWTLFREHFDGRVDWNHKTEVYLAACSTAGE
                     GGSAFVHGHFAGGVRAAFPTRVRVWASTTAVRGRDLSGDWVQL"
     gene            3602..5911
                     /locus_tag="PLH85_08855"
     CDS             3602..5911
                     /locus_tag="PLH85_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64710.1"
                     /translation="MLTPAPDVRTVSGDPLRLLGESAAALTDGRVCLAERIHPDDQDV
                     AARLFDPHAPAADGPLHLRLRRADGRIVVTCATCTHHASADGQHFELHLSDSRDLPRT
                     LDDVATLSVRAMLENTDDYIYFKDRHHVFAAASQTLVSLCEPVGHWTDFLGQTDYDVF
                     PEAYADVYYRLEKQVFAGAQVAHEVQPYLHRDGVTRGWVDNRKYPVLDADGRIIGLFG
                     IARDITDKVLAEAALRESEYFFRESQRAAHIGSYKFDVGSGRWTASEVLDDIFGLAPA
                     PDRQLQDWLDLIHPDDRDGMHRYLLDHVLTQRQPFNREYRILRRCDGSLRWVLGCGQL
                     ELDATGHPTHMFGTIQDITARRLLDEELADYRAHLEQRVDERTRELQDAKNAAEAASL
                     AKSAFLANMSHEIRTPLNAITGMAHLIRKEPLSPQQAERLAKLETAGQHLLEILNAVL
                     ELSKIEAGKFALEEQPVRLAQVVADVVALLQWRSAGKGVVLRSEVGPLPANLLGDATR
                     LQQALLNYTANAVKFTERGEIVLRAGIVEDGPADVLVRFDVEDTGIGITADALPRLFN
                     AFEQADNSTTRRYGGTGLGLAITRKLAQIMGGEAGADSQPGRGSRFWFTARLRKGPTA
                     AEAARPDPAATLLALSTRHAGARVLLAEDEPINQEITQLLLEEARLVADIAENGEAAV
                     AKARDNDYAVILMDMQMPRLDGLDATRQIRQLPRHARTPILALTANAFAEDEARCRQA
                     GMNAFIAKPIHPEALYGEVLRVLEGEYAS"
     gene            complement(6138..10229)
                     /gene="hrpA"
                     /locus_tag="PLH85_08860"
     CDS             complement(6138..10229)
                     /gene="hrpA"
                     /locus_tag="PLH85_08860"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNB64711.1"
                     /translation="MAAPHDRPDAVRQDPYSLLEHCLTADRPALRRDLRRLPRRPRTL
                     EDLPEALRLRIADSAARRIAREQARPRPDFPDELPVNQRRDEIAAAIRDHQVVIVCGE
                     TGSGKTTQLPKICLELGRGVAGLIGHTQPRRIAARATASRIAQELRTELGSAVGYKIR
                     FTDRIGPQSYIKLMTDGILLAETQGDPLLSAYDTLIIDEAHERSLNIDFLLGYLRQLL
                     AKRPDLKLIVTSATLDAERFARHFGTPERPAPVIEVSGRLYPIELRYRPVERDAPKPL
                     RPGEAPRPEPRGPDRDLYDALVDAVDEAQRSGPGDVLVFLPGEREIREAAEALRKAHH
                     AAGTEILPLFARQSAADQARVFAPSKGRRVVLATNVAETSLTVPGIRYVVDTGLARVK
                     RYAHRNKVEQLQIEKIAQSAARQRAGRCGRVSDGICFRLYDEDDFNKRPAHTDPEILR
                     SSLAGVILRMKALKLGEVADFPFIDAPLPRMVADGYQLLAELGAVEEGGADEAGSARM
                     ALTPIGRELARLPLDPKIGRMILAARDRGCLAEVLVIAAALSTQDPRERPPERQAAAD
                     QAHARFRGPEHEQKSEFLWFWNLWKAWDEVLRHESASKQKAWCKQHFLSWLRLREWRD
                     VHGQLHALCAEHGWKENTEPAAYEALHKALLAGLLGHVGCKIEDATGPAAGSYLGARG
                     IKFWPHPGSSLAKKAGKWIMVAELVDTSRLFGRCLARMEPEWLEEVGAHLLKRQISEP
                     HWSKSHGAVRAWERATLYGLTVYARRGVSYRDIDPALCRELFIREGLVQGEIAEGPAR
                     GMAFLAHNRRLVAEIERLEHKSRRPDVLVDEALIHAFYDAKLPADVVDLASFEAWRKD
                     AERKAPKVLQLTRDQLMRHDAEGITTDRFPAHLDVLGQKLKLTYLHAPGEADDGVTLT
                     VPLAMLNQVPAHRCEWLVPGLLEEKVTALLRTVPQKHRHRLQPMADSAAAFMERFEAG
                     EFDLDEPFLKALQGFVEERVSLKLPMESFRPENLNPHCFMHFRVQDEHGRVLGQSRNL
                     AELRSRFRDQVAARFQSARIVPAADVPATVAPVGKAPAAVDATSPVAGGALAGLTGWT
                     FGPLPELLEVKVAGREIVGFPALHDDGASVSLRPYDTPEEAAKVHRGGLARLFALALA
                     AQVKAIEKLPGLRELALQFLPYGTEAELKAQLVAATLERCCLMAPLPADADAFARCCQ
                     DAKPRITLVAQELMRLAGQLIAEHATLGKRLAGLKTFPEVVADITAQAARLMPKDFIV
                     ALPYERLAQVPRYLKGASVRIDKLRNNAARDAQLQADWKRLAQPFEREWLAKARAGVT
                     DPQLEEFRWLLEELRVGLFAQELKTPMPVSVKRLQKIWDSRPR"
     gene            complement(10257..10661)
                     /gene="crcB"
                     /locus_tag="PLH85_08865"
     CDS             complement(10257..10661)
                     /gene="crcB"
                     /locus_tag="PLH85_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNB64712.1"
                     /translation="MPDHSHSFPTLALVAIGVGAALGAWLRWWLGLRLNPVLAQMPLG
                     TLAANLLGGYLVGVAVAFFALRPDIPPEARLLVVTGFLGGLTTFSSFSAEVVHLISTA
                     RYAWAAGAAAVHLFGSLTMTALGMWTVHAVRG"
     gene            complement(10729..11739)
                     /locus_tag="PLH85_08870"
     CDS             complement(10729..11739)
                     /locus_tag="PLH85_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNB64713.1"
                     /translation="MSPGSPATMRAMVLHQPRTPLQLERLPVPAPPPGTVRLKVEACG
                     VCRTDLHLMEGELPDPVWPMIPGHEIVGRVDVVGAGVSGFAPGQRVGVPWLGWTCGTC
                     GFCRSGRENLCAQARFTGYQIPGGYAEYTVADARYCFPLPDGGEAAALAPLLCAGLIG
                     YRALRMADDAQKVGLYGFGAAAHLVAQVLRHQQRPFYAFTRPGDIESQAFARQLGATW
                     VGDAATPPPERLDAALLFAPAGALVPAALRHVDKGGCVICAGIHMSDIPSFPYADLWG
                     ERTIRSVANLTRRDGLEFLALAPQVPVRAEITPFPLARANEALDALRRGALQGAAVLT
                     MD"
     gene            11961..13496
                     /locus_tag="PLH85_08875"
     CDS             11961..13496
                     /locus_tag="PLH85_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB64714.1"
                     /translation="MDSLAIPPALHYQPRYDCRDGALVGAKLYVARPERSDNVVLTAL
                     DDACLARLHSACRQVADWNAHHGLDLILTLGATLEVLATPTFRQALNGLILDARLDAH
                     SLELNIITRTGEIPDEQAAALHHLKRCGVRFAISAASPANARLAWLRRLPVDCLEVPA
                     ALVQDVTADPEGVAIIRALVTRAHKMGQRVSAKGIADRHTAGVLVASDCDFVQGPLLG
                     GPMTAEAFGALLAAGERLNPELIRGPRPDRTLLLVDDEENILNALRRLLRRDGYTLLT
                     ASSGQQALDLLATHPVDVIVSDQRMPGMTGVEFLRKAKDMVPDTVRMVLSGYTELQSV
                     TDAINEGSIYKFLTKPWDDAMLRANIDEAFHRKALVDENLRLDAELHRANAELARINE
                     RLAALLAERERQLDVDESALHLTQAALAALPAPVLGTDPGGMIAFANAAADKLFPAHA
                     PLIGLGVDEALPPDLRDLALRGEGATDYRDGARHFHVVAHPLHAVGTCRGTLLSFTDR
                     SGA"
     gene            13493..13690
                     /locus_tag="PLH85_08880"
     CDS             13493..13690
                     /locus_tag="PLH85_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64715.1"
                     /translation="MSEATEHVDTPPDGAAEEAERAAELERRREAIRARVMHLFRHQA
                     DDPGAQALLHAVLAFRQEQLK"
     gene            13687..14538
                     /locus_tag="PLH85_08885"
     CDS             13687..14538
                     /locus_tag="PLH85_08885"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB64716.1"
                     /translation="MNPMTAAPTLTDTLARIQRLPVMSAVAMEVLQSLNGADLDVDAL
                     ARRIAQDQAIAARVLRVANSPFYGMAGRVASIHDAIMVLGLSAVRSLVLAAAVVTALP
                     AGHCPGFDERRFWHHVIGTAAAAEAMGKALRRSPGHLFLAGLIHDIGRLALVTLYPAD
                     YAEVLRRAGERDAQLIDVELEIFGFDHAEAGAALARRWNFPDEIADALACHHAPERSQ
                     PGGLAGVLHYADAIAQALDLDGETDSQIPRLHAATVDALGLGRDDLHGVLAGTRARFD
                     SYCLLLG"
     gene            14543..16354
                     /locus_tag="PLH85_08890"
     CDS             14543..16354
                     /locus_tag="PLH85_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB64717.1"
                     /translation="MNPRPYALPVSLFPLADSLPSGLVLHRSDRILFANRAMSRLTGY
                     TADELAQMDFWAFFPKAAQGKVQEWGRMRLQGEHPPARYETLIVIKSGEERWVELTLS
                     CEAIDGARTVLCSYLDITDRKRAEAAQAQSRQLLAQIIDGDPVPTLVINARHIVTHWN
                     QACEQITGIRAADIVGTRKHWTAFYDHERPIMADLIVSGEIETITRHYGQKKLRRSPV
                     VASAYEAEDFFPHFGENGRWLYFTAAPLRNAAGATIGAIETLQDITERRKAEDELRQM
                     HTSLEAKVAQRTVELQDAKATLERDIARRETAEAELRARYSELKDLNSRLSDTQQQLV
                     QSEKMASIGQLAAGVAHEINNPIGYVNSNIRSLKSYIAQLLGVVDAYQAREAALPEPD
                     RAALAAARKAADFDFLREDVTQLLAESEEGTERVRKIVQDLRDFSRTEATQDWQSVDL
                     RRGLDSTLNIASNEIKYRAEVVRDYGEMPLVECLPSQLNQVFMNLFVNAAQAMPEGHR
                     GLITVRTGTEPTGEAVWIEVADNGSGMPADVMARIFDPFFTTKPVGKGTGLGLSLSYG
                     IIQKHHGHIGVTSTPGAGTTFRIVLPVHRALTDGATP"
     gene            16351..17652
                     /locus_tag="PLH85_08895"
     CDS             16351..17652
                     /locus_tag="PLH85_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64718.1"
                     /translation="MSEAAAPAFTLLCVDDEANILSALRRLFRPHGYTVLTAGGGEEG
                     LEILAREPVDLIISDMRMPVMDGARFLGEARKRHPDTVRLLLTGYADMESTIEAINAG
                     QISRYISKPWNDQDVLLTVREALERKQLEKEKARLEALTRSQNDELKSLNASLEQKVE
                     ARTAELRIAHDKLKQNFLTSIRVFSNLIELREGAMSGHSRRVADLARKMAIHLGFNAT
                     DAQDVMLAGLLHDVGKIGLRDELLSKPVSHMTGDELGQLRRHPVAGQSALMALENLRK
                     AGTLIRAHHERWDGQGYPDGLSGLAIPIGARLLAVANDFDGLQIGTLTPRKLRQEEAL
                     AFIQQNRGKRYCPQSVDALAAVLGSTESAPAVGNIVSTAALKPGMVLARDLVTPEGVM
                     LLAADFVLDDGLIRQLRELEASEGRRLAIPIRAERKTTASM"
     gene            17713..18231
                     /locus_tag="PLH85_08900"
     CDS             17713..18231
                     /locus_tag="PLH85_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB64719.1"
                     /translation="MIFSFTRKREFEQVVRAYSADLYRFAYWLCRDRFVAEDLVQESF
                     ARAWSNWDSLRDKSAPKPWLLTILRNEHARLYERKQFDYVDEQPEELAIAADHDPVAL
                     HELEDLIGRLPLSLREPFLLQTLGGFSCGEIAAQLDTTEGAVMVRLTRARQGLRQLLD
                     GAAAPRHTGSAS"
     gene            18228..18971
                     /locus_tag="PLH85_08905"
     CDS             18228..18971
                     /locus_tag="PLH85_08905"
                     /inference="COORDINATES: protein motif:HMM:NF023287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HNB64720.1"
                     /translation="MNAQNPARDVFASPMNCLEFRRLKLADPHRLSAAALAHQRECAL
                     CQAFARRVDTQEQRIATVLDIPLPDGLADRVLVRVHHGRPRPWRFLALAATVVLSVGL
                     SFNLWHDYPRDDYARFAIDHTLHEPESFTDHRLADPSQFRLALARFGAELEAPVGEVL
                     YMKLCPVPGGTGWHIVIRTEHGQATLLLIPRKAGEGSAQGKPLEAEWKGMTALAQPGG
                     DGYYAIVADSPERAQAIAAMMKERIRWRI"
     gene            complement(18981..21116)
                     /locus_tag="PLH85_08910"
     CDS             complement(18981..21116)
                     /locus_tag="PLH85_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB64721.1"
                     /translation="MTEASRARILIVDDDPLTRLMAGEALREGGYAVQEAEDGQQALE
                     LFSARRPDLVLLDVVMPGLGGFEVCARLRALDAGLLVPIIMLTGLDDSESVQRAFDAG
                     ATDFISKPINWTLLRFRVRYVLRSADVMKELVRNKESLSNAQRVARLGSWEWWPEQGS
                     IQRSEQYFRLFGQTAASFGDDAQALVDHVYLPDRATVAAALDEAARGHGYQLTYRVVW
                     PDGSVRTLHETAEPAPSSDPARGRWVMEGSVQDITQQVEAQRRIRQLAYFDSLTGLPN
                     REFFRESLLAAVSRCRRNETGCAVMVLDVDRFARINDSLGPDRGDQVLQVIAHRLREC
                     MGDRAPVEPAGGGGLPDLKVARLASDEFAILVQEAGSPSMLLGVAEGLLATVRAPIHV
                     PGQEIALSARIGVAMMPDHASDADALLKAAETALNRAKTQAGEQVALFSEEMKVAAFL
                     RFSLESDLRRAIENNELTVVYQPIVDVRRRAIVKAEALIRWKHSLRGMIPPPEFISLA
                     EESGLILPLSRRLMDTVFADVARLEPVAPEGFRASVNLSGVNFMDVQLIPMVNGLLAD
                     RGVPATRLEFELTETVLMRDLDYATTILARLREMGVRVAVDDFGTGYSSLAYLHSLAV
                     DTLKIDRSFVNELDSGQGGVIVEAVIGLAQSLGLDVVAEGVETAAQLGALRQRGCHLI
                     QGYLFARPMPVDDLQRLLRDGADFSSILA"
     gene            complement(21109..23919)
                     /locus_tag="PLH85_08915"
     CDS             complement(21109..23919)
                     /locus_tag="PLH85_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64722.1"
                     /translation="MNPLNALRDLPIGRKLSTVVRLGVLIALGVGFLALFVTEIHNEI
                     RRVNNEGRVLSEIIADGAASPLRFDDPRVADSLLASLRHHERVATALLVRPDGRVFAA
                     YPAGVGAAQARVEALKAAAEPGRLGWGLTHLVVAQGIESDGENLGRLIIEFDLHPVLG
                     QFLAWFGYALFGLGGALLAASLLTRRMQAMIVEPIQRLAEVMREVGRYKRYDLRAPAG
                     YADEVGELIAGFNGMLAEIATRDQELTLHRAQLESEVAVRTRELVAAKEQAEAANRAK
                     SQFLANMSHEIRTPMNGVMGMIGLLRQTSLAARQGHFVDMLDDSARALMEVINDVLDV
                     SKIEAGRLELESLAFSLRDTVDQVASLFATSAQQRGIRLRLKVEQDVPDMVRGDALRV
                     RQVISNLVSNAQKFTEHGSIAIQLRRLPCAHPGRVRIRATVRDTGIGVPRGMESRLFQ
                     SFSQADNSMARRFGGTGLGLSIAKQLVTLMDGDIGYDSAPGEGATFWVEIELGAVTQG
                     DAHAVLDGRRALLAMGDPASREALAEQIVFMGGAVEIAPDQAAVTARLASGAVFQWIV
                     TDAGFDGGKGFALLEMLREAPSAPPRLAVVGTGREEAEQARIAGAAEVLAGPLTGVAI
                     RRALLGQERDPDDTSVVPLTARVLVAEDHPVNREIAVAVLESLGCRVAVAENGREAFE
                     ACRRERFDLVLMDIQMPEMDGRQATVAIRADEDFRCLPRVPVVALTANALREDRDACL
                     AAGMDDYLVKPVSREQLGETLAKWLTTFVPVEPAPSLLASPRPAPSGHVEAPAALDMD
                     VLLGLPGVNRNPSSPMLVRLVDLFATETERHVASVRSSFEAGDWSAMRAVAHKMKSGC
                     LALGAVRLAGCARALDDHIREGGVPAAGEVEALAEAWQACRAVMVARGLLAAGEAERA
                     AVPPSPDTPPNSPAPTAPQDHD"
     gene            complement(23921..24466)
                     /locus_tag="PLH85_08920"
     CDS             complement(23921..24466)
                     /locus_tag="PLH85_08920"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB64723.1"
                     /translation="MTFRILRFLLVLWAALGAGLPAVAVPPGEYQIKAAYILNFARYV
                     EWPAARLPGGAVLRVCLLGRDPFDGALHGIDGRLVQGHEVRVRQADSLEQAVDCHVVF
                     VAESEERRTGLILRTLAGRGVLTVSDIDGFAEAGGGIGLVTEEDRVRFDINQAALTRD
                     TLKASSQLLRLGRNVFGLKGL"
     gene            complement(24477..26501)
                     /locus_tag="PLH85_08925"
     CDS             complement(24477..26501)
                     /locus_tag="PLH85_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB64724.1"
                     /translation="MNPTVLSIAVLSAILALPVHAGERELDDLSLEELVKLEVASVAR
                     KAQKLSDTPAAVTVLSADDIRRSGARSIPEALREVPGVNVAQIGAARWAVSVRGFNGR
                     FSNKLLVQIDGRSVYSPLFSGVFWEAIDVLLEDVERIEVVRGPGASLWGANAVNGIIN
                     IVTRKATATQGTLASVTVDDRGAPVVAARQGFTLANGAALRVFAKGGEISSTRREDGD
                     AVGDKLTGWLAGFRMDSAVGGRNTWSLQGNAYRRSGGDSAVGLVAEAPSGNAFAPQFD
                     GANLLGRYNWGLAGGEATLQAYLDRQQADVGSYGTGAVTTADLDLQHRLAPVGKHALM
                     WGLGYRAFRAEIESRTPMLWMTPNRRDSHIASAFAQDEITLLPRRLMLTLGGRFEYSS
                     LSGFEPQPTVRLMWTPTTTDSVWAGWSRAARTPSIGETDANLVNRIVPLPPGFGPTSA
                     AIVSQPAADWNRRAERVVAVETGYRRNLGNGSVEAVLFHQRYSGLIGGATDPAGLIVP
                     GIAIGVPPGYLPIQRVLRSNNVSGCSSGLELGMDVPVTAWFRLQASYTLMNTSIDPAS
                     DVALATFGGNLKYSAPNHWASVHGVFALGGGRDVDLMLRRVGRVGEGDIPAYTAVDLR
                     YAWKLNRTFELAVVGQNLFDPLHLEYVSDVFPGVPAYLPRRGFVQGVWRY"
     gene            26809..>27409
                     /locus_tag="PLH85_08930"
     CDS             26809..>27409
                     /locus_tag="PLH85_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNB64725.1"
                     /translation="MTAPTPRGEDNAASNFIRNIVDEHRRTGKWSGRVETRFPPEPNG
                     YLHYGHAKSICLNFGLALSYSGACHLRFDDTNPTKEEQEYVDAIIEAVQWLGFDWGEH
                     LYFASDYFDKMYAMAEYLITVGKAYVDSLSAEEMRAYRGTLSAPGQDSPYRNRSVAEN
                     LELFQRMRAGEFPDGTHILRARIDMASPNINLRDPAIYRI"
BASE COUNT         4518 a         9884 c         9133 g         3874 t
ORIGIN      
        1 ctggcgcagg ctgtggcgca gctccacgtg ggcgatctcg tgggccagca cgccggccac
       61 ctcgtccgcc gagccagcct ggcgcagcag cgccgagtac accacgacca ccccgcccgg
      121 cgccgcgaag gcattcacct ggggactctg caccaggtgg aattgcaggg tttcgccggg
      181 ccgcgtgagg cgccggccga tggcctgcac ggcctccgcc gccgcgcccg tttcgatgaa
      241 gggcccgtcc tggcgcgtcc gggccagcac agcctcgccg atctgcgcct cgacggacgc
      301 ggggatgtgc gccacgaccc gatccaccag cccgtcgaag ccccacacgc caccgaccac
      361 cagggccagg cacgcgagga tcaggacccc gtaggcgaag acacggtgca gaccggcccg
      421 ctgccgcgcc tgatcgcccg tcgcgaacag ccccggcagc ccattgcgca gggggccgac
      481 cgccgcgtcg tccacgatga gcaggtgatc gccgtctgcg ccggcccacg cgagggccca
      541 ctggctgtgg ttgaagcccc gcggcgaggc ctgcaggcgc gccgcgggca catgcagcgc
      601 ggcgccttcg accggagcga cgacgagctg cccggcggcg gccttcaggg tcacggcgcg
      661 gccgtgggcg tcgccgccgg gcgggtaatg acgcgccgcg acgggcgtgc tcactcgtcg
      721 tcgccgagca gggagcccag gcccaccccg ctcagcagcg agccgccttc cttaccgccg
      781 ccgcccggca cggcgcggga cgccaatacg atgcggtcgg ccatgcgcga caggggcagg
      841 gactgcagcc ataccttgcc gggcccggtg agggtggcga agaacaggcc ttcgccgccg
      901 aacagggccg tcttgatctt gcccgcgtac tggatgtcga aatccacact gggctggaag
      961 gccaccacgc agccggtgtc cacgcgcagg cgctcgcccg gcgcaagggt cttctccagc
     1021 agcgtgccgc ccgcgtggat gaaggccatg ccatcaccgt ccagcttctg catcacgaaa
     1081 ccttcgccac cgaacaggcc tacgccgatc ttgcgctgga aggcgatgcc cagagccacg
     1141 cccttggcgg ccgccaggaa ggcgtccttc tggcagatca gggtgccgcc caccgtggac
     1201 agatccaccg ccacgatctt gccgggatag ggcgccgcaa acgccacccg gcgcttgccc
     1261 gtgccttcgt tgtggaacac cgtggtgaac aggctctcgc cggtgagcag acgcttgccg
     1321 gcccccaaca gcttgccgaa cagcccgctc tgctgggccg aaccatcacc gaagaccgtg
     1381 tccatggcga tgccgtcctg catgtacatc atcaccccgg cctcgccgat ggccgcctcg
     1441 cccggatcga gctccacctc cacgtactgc atctcgtggc caaaaatttc gtaatcgacg
     1501 acatccatgg gcatgacaga gtctccatct gaaaggaagg gaagtgtatc gggccgcgcg
     1561 gtctgcgcgc acgcggaagg acatgctgcg cgacgcatgc ttcaagatcc gcattgcatg
     1621 acgatgacgg cggtgcatgg cggacgcccg cgctacacca ggccggacgc gatgttgatg
     1681 gtcaaggcca gcacggtggc gttgaagaag taggacagca cacagtggaa aagaccggtg
     1741 cggcgcatgg cgcgggtcgt gaaactcacg tcggcggtct gcccggaggt gccgatgacg
     1801 caggcgaaat acaggaagtc accgtaatcc ggcgcgtgcc cacccggaaa cgcgaggccg
     1861 ccgctgccct gtcgggcttc ggccagatag aagtcatggg cgtaatgctg ggcgaacatg
     1921 acctgggtga gcaaccagga cgacaggatg gtcacggcgg caaaggcgat gtgcaggagc
     1981 ctggatggac cggtcagatc cttcgccacc gccagttcgg ccacgatggc gcccaggctc
     2041 atcaccgccg ccaggagcac cagcgcgagc acggtacgct gcccggcgtc ctggtccaac
     2101 gcccggcgct gcatgcgttc gtgcgttgag cggaacatca tcgtcaggga cagggccaga
     2161 tacagccagc agccgatgtt ccaggcaagg atgccgcggg gtaccggctg atgtgcccag
     2221 gtatcgggta acgccacgag cgcgagcacg ccacagatcg tgctgaagat gagccggggg
     2281 cgcgcagtca ccgcccgcac cggcgcactg cgatgcagcg ccttcatgga ggggcacctg
     2341 gatcggtcaa tcccggtcgg tcgccgtcgg ggcggccgct gtgccgacgc acgcgatcaa
     2401 cgtcccgccg gatcagacat cgggccgcag gcagccggcc gcccgcattg cggcgtaggt
     2461 ttcggcgagc gcctgatcgg tcgccacggt ctcccgcaac tggcggatga agccggcgca
     2521 ggccggccgc gacgcgacca gttcggccgt cacgagggca cgctcgtggg tggaggcatg
     2581 ggagggggac gaacatcccg ccacgacggc gagggcgagg atcgacaggc tgaggttcag
     2641 gcgcacgggg tgtcctccgg caaggggaag tggctgcaga cggtagcaga gtttggccgc
     2701 cgcgtgcgtc cgttcccggt cgcggacagc ggcctttcgc ccggcgcagc gccgcggatc
     2761 gcaggtcggc gcctcgcacc cggcctgaag cggtcgccct gcgtcgcaga ccaggaggcg
     2821 gtctgtcgcg gcacgggccc tgcgctcccg tgcccgaccg ggccggccgg ggcacgcacc
     2881 gtgtccgcgc gtactagagc tgcacccagt cgccggagag atcccgcccg cgaaccgcgg
     2941 tggtcgacgc ccacacgcgg acccgggtcg ggaaggccgc ccgcaccccg ccggcgaaat
     3001 ggccatggac gaaggcggag ccgccttcac ctgcggtcga acaggccgcg agatacacct
     3061 ccgtcttgtg gttccagtcc acccggccgt cgaagtgttc ccggaagagc gtccacttgc
     3121 cgcagccact gagcttgttg gcgttaccgt gcccgacgat caccaggact tccacgccgg
     3181 tgccaccgtc gccctccacc caggtatcgc ccggtgtcag cagacggtag tcaccctcag
     3241 ggccgccgat ggttgcccgc cccgcgatga ccgccttgat cgcccgcatc accacgtcgt
     3301 cttcgggttc cagagtcagg atgtcgtaca tgttctgagt gctcctgttg gggtttgagt
     3361 gctgccgtcc ggcgcaagcc ccgcagccgg tcagcccgaa atccatcata gataaccgga
     3421 ctggatggcg aaaggcccgt cacggatgtc ctcgcgtccg cccccgggcg gcgggatacc
     3481 ggcaccttgt ccgggctcaa gactttcctg ccacgtgtcg ctaccattcc gtgaatcgaa
     3541 caggcaggca ccgacgatgg acacggtcaa tcaagcgcca cccggcgtgt ggctccgcgg
     3601 catgctcacc cccgcgccgg atgtgcgcac ggtctccggc gatccgctgc gcctgctggg
     3661 cgagtcggcc gccgccctca ccgacggccg cgtctgcctg gccgagcgca tccatcccga
     3721 cgaccaggac gtggccgccc gcctcttcga cccccacgcc cccgccgccg acggccccct
     3781 ccacctgcgt ctgcgccggg cggatggacg catcgtcgtc acctgcgcca cctgcaccca
     3841 tcacgccagc gccgacggcc agcatttcga gctgcacctg agcgacagcc gcgacctgcc
     3901 ccggaccctc gacgacgtcg ccaccctcag cgtgcgggcc atgctcgaga acaccgacga
     3961 ctacatctac ttcaaggatc gccaccacgt ctttgccgcc gccagccaga ccctcgtgag
     4021 cctgtgcgag ccggtgggtc actggaccga ctttctcggt cagaccgact acgacgtctt
     4081 ccccgaagcc tacgccgacg tctactaccg cctggaaaag caggtcttcg ccggcgccca
     4141 ggtggcccat gaagtgcagc cctacctcca tcgggacggt gtcacccgcg gctgggtgga
     4201 caaccgcaaa taccccgtac tcgacgccga tgggcggatc atcggcctgt tcggaattgc
     4261 ccgggacatc accgacaagg tgctcgccga agccgccctg cgggaaagcg aatacttctt
     4321 ccgcgaatcc cagcgcgccg cccacatcgg gtcctacaaa ttcgacgtgg gcagcggccg
     4381 ctggaccgcg tccgaagtcc tcgacgacat cttcggcctc gcccccgcgc ccgatcgcca
     4441 actccaggac tggctggatc tcatccaccc ggacgaccgg gacgggatgc accgctacct
     4501 gctggaccac gtcctcaccc agcgccagcc cttcaatcgg gagtaccgca tcctgcggcg
     4561 ctgcgacggc agcctgcgct gggtgctcgg ctgtgggcaa ctcgagctcg atgccaccgg
     4621 ccacccgacg cacatgttcg gcaccatcca ggacatcacc gcccgccgcc tgctggacga
     4681 ggagctcgcc gactaccgcg cccacctgga acagcgggtc gacgaacgta cccgcgaact
     4741 ccaggacgcc aagaacgccg ccgaagccgc gagcctcgcc aagagcgcct tcctggccaa
     4801 catgagccac gagatccgca cgcccctcaa cgccattacg ggcatggccc acctgatccg
     4861 caaggagccc ctcagccccc agcaggccga gcgcctcgcc aagctcgaga ccgccggtca
     4921 gcacctgctc gagatcctca acgccgtgct tgagctctcc aagatcgaag ccggcaagtt
     4981 cgccctcgaa gaacagccgg tccgcctcgc ccaggtggtg gccgacgtgg tcgccctgct
     5041 gcagtggcgc tctgccggca agggcgttgt cctgcgcagc gaagtcggtc cgctgcccgc
     5101 gaacctgctg ggcgacgcca cgcgtctgca gcaggccctc ctcaactaca ccgccaacgc
     5161 ggtcaaattc accgagcggg gcgagatcgt cctgcgcgcc ggcatcgtgg aagatggacc
     5221 ggccgacgtc ctggtccgct tcgacgtgga ggacacgggc atcggcatca ccgccgacgc
     5281 gctgccgcgc ctcttcaacg ccttcgagca ggccgacaac agcaccaccc ggcgctacgg
     5341 tggcaccggt ctgggtctcg ccatcacccg caagctcgcc cagatcatgg gcggcgaagc
     5401 cggtgccgac agccagcccg gccgtggcag ccgtttctgg ttcacggcgc ggctgcgcaa
     5461 gggtccgacc gcggcggagg ccgcccggcc cgacccggcc gccaccctgc tcgccctcag
     5521 tacccgccac gccggcgccc gcgtgctcct cgccgaggac gaacccatca accaggagat
     5581 cacccagctc ctcctggaag aagcgcgcct cgtcgccgac atcgccgaaa atggcgaagc
     5641 cgccgtcgcc aaggcccgcg acaacgacta cgccgtcatc ctcatggaca tgcagatgcc
     5701 ccgcctcgac ggcctggacg ccacccgcca gatccgccag ctcccccgcc acgcccgcac
     5761 ccccatcctc gccctcaccg ccaacgcctt tgccgaggac gaagcccgct gccgccaggc
     5821 cggcatgaac gccttcattg ccaaaccaat ccatccggag gccttgtatg gggaggtgct
     5881 gcgggtgctg gaaggcgagt acgcgtcctg aagcaggcgc gctgccccgc ggcgggaccg
     5941 gagggcgatc cgcgcccgcg cgggcgccag atcggcccgc cgccatctcc ccctgcatcg
     6001 cccccacaaa gacagaaggc cgctcattgg cggccttccg tgttctccac cgatgtggag
     6061 gctgtgccca ttgcgggtca attcttttat agacagaggg gcggacatcg tcaaatttga
     6121 tgacgtgacg cccgcggtca gcgcggccgg ctgtcccaga tcttttgcag gcgcttgacc
     6181 gagacgggca tgggcgtctt gagctcctgg gcgaagaggc cgacgcggag ctcttccagc
     6241 agccagcgga actcttcgag ctgcggatcg gtgacgccgg cgcgggcctt ggcaagccat
     6301 tcccgctcga agggctgggc gaggcgcttc cagtcggcct ggagctgggc gtcgcgggcc
     6361 gcgttgttgc gcagcttgtc gatgcgcacg ctggccccct tgaggtagcg cggcacctgg
     6421 gcgaggcgct cgtagggcag cgcgacgatg aagtccttgg gcatcaggcg ggcggcctgg
     6481 gcggtgatgt cggcgaccac ctcgggaaag gtcttgaggc cggcgaggcg tttgcccagg
     6541 gtggcgtgct cggcgatcag ctggccggcg aggcgcatga gctcctgggc caccagggtg
     6601 atgcgcggct tggcgtcctg gcagcagcgg gcaaaggcat cggcgtcggc agggagcggt
     6661 gccatcaggc agcagcgttc gagggtggcg gcgacgagct gggccttgag ttcggcctcg
     6721 gtgccgtagg gcaggaactg caaggccagc tcgcgcaggc ccggcagctt ttcgatggcc
     6781 ttgacctggg cggcgagggc cagggcgaac aggcgtgcca gtccgccgcg atggaccttg
     6841 gcggcctcct ccggcgtgtc gtagggacgc agggagacgc tggcgccgtc gtcgtggagg
     6901 gccggaaagc cgacgatctc gcggccggcg accttcacct ccaataactc gggcagcggg
     6961 ccgaaggtcc acccggtgag gcccgccaga gcaccgcccg cgacgggcga cgtggcgtcg
     7021 accgcggccg gcgccttgcc gaccggggcg accgtagccg gtacgtcggc ggcagggacg
     7081 atgcgggcgg actggaagcg ggcggccacc tgatcgcgga agcggctgcg cagctcggcc
     7141 aggttgcggg actggccgag gacgcggccg tgttcgtcct gcacccggaa atgcatgaag
     7201 cagtgggggt tgaggttctc gggccggaag ctttccatgg gcagcttgag ggacacccgc
     7261 tcctccacga aaccttgcag ggccttgagg aagggctcat ccagatcgaa ttcgccggct
     7321 tcgaagcgct ccatgaaggc cgccgcgctg tcggccatgg gctgcaggcg gtggcggtgc
     7381 ttctgcggca cggtgcgcag cagggcggtg accttttctt cgagcaggcc ggggaccagc
     7441 cattcgcagc ggtgggcggg cacctggttg agcatggcca ggggcacggt gagggtgacg
     7501 ccgtcgtcgg cctcgccggg ggcgtgcaga taggtgagct tgagcttctg gcccagcacg
     7561 tccaggtggg cggggaagcg gtcggtggtg atgccctccg cgtcgtggcg catgagctgg
     7621 tcgcgggtga gctgcaggac cttgggcgcc ttgcgctcgg cgtccttgcg ccaggcttcg
     7681 aagctggcca gatccaccac gtcggctggg agcttggcgt catagaaggc gtggatgagc
     7741 gcttcgtcca ccagtacgtc gggacgccgg gacttgtgtt cgaggcgttc gatctcggcc
     7801 acgaggcggc ggttgtgggc caggaaggcc atgccccgcg cggggccttc ggcgatttcg
     7861 ccctgcacca ggccttcgcg gatgaaaagc tcacggcaca aggccgggtc gatgtcccgg
     7921 tagctcacgc cgcggcgggc gtagacggtg aggccgtaga gggtggcgcg ctcccaggcg
     7981 cgcacggcgc cgtggctctt ggaccagtgg ggttcggaga tctggcgctt gaggaggtgg
     8041 gcgccgacct cctccagcca ctcgggttcc atgcgggcga ggcagcggcc gaagaggcgc
     8101 gaggtatcca ccagctcggc caccatgatc cacttgccgg ccttcttggc caggctggag
     8161 ccgggatggg gccagaactt gatgccgcgg gcgcccaggt agctgccggc ggcggggccg
     8221 gtggcgtcct cgatcttgca gcccacgtgg ccgagcaggc cggcgagcag ggccttgtgg
     8281 agggcctcgt aggcggcggg ttcggtgttc tccttccagc cgtgctcggc gcacagggcg
     8341 tggagctggc catgcacgtc gcgccattcg cgcaggcgca gccaggacag gaagtgctgc
     8401 ttgcaccagg ctttctgctt gctggcgctt tcgtggcgca gaacttcgtc ccaggctttc
     8461 cacaggttcc agaaccacag gaattcggac ttctgctcgt gctcggggcc gcggaagcgg
     8521 gcgtgggcct ggtcggcggc agcctggcgt tcgggcgggc gctcccgcgg gtcctgggtg
     8581 gacagggcgg cggcgatgac cagcacctcg gcaaggcagc cccggtcgcg ggcggccagg
     8641 atcatgcggc cgatcttggg atccaggggc aggcgggcca gttcgcggcc gatgggggtg
     8701 agcgccatgc gggcgctgcc ggcctcgtcg gcaccgccct cctccaccgc gcccagctcg
     8761 gcgaggagct ggtaaccgtc ggccaccatg cgcggcaggg gcgcgtcgat gaaggggaag
     8821 tccgccacct cgcccagctt gagggccttc atgcgcagga tcacgccggc cagggaggag
     8881 cgcaggatct ccggatcggt gtgggcgggg cgcttgttga agtcgtcctc gtcgtagagg
     8941 cggaagcaga tgccgtcgct cacccggccg cagcggccgg cccgctgacg ggcggcggac
     9001 tgggcgatct tctcgatctg gagctgttcc accttgttgc ggtgggcata gcgcttgacc
     9061 cgcgcgaggc cggtatccac cacgtagcgg atgcccggca cggtgaggga cgtttcggcc
     9121 acattggtgg cgagcacaac ccgccgcccc ttgctggggg cgaagacacg ggcctggtcg
     9181 gcggcggact ggcgcgcgaa gaggggcagg atctcggtgc cggcggcgtg gtgggccttg
     9241 cgcagggctt cggccgcctc gcggatctcc cgctcgccag gcaggaagac cagcacgtcg
     9301 ccggggccgc tgcgctgggc ttcgtccacc gcatcgacca gggcgtcgta gaggtcccgg
     9361 tcggggccgc ggggctcggg gcgtggcgcc tcgccggggc gcagcggctt gggggcgtcc
     9421 cgctccaccg ggcgatagcg cagctcgatg gggtagaggc ggccggacac ctcgatgacc
     9481 ggcgcaggcc gctccggcgt gccgaagtgg cgggcgaagc gctccgcgtc gagggtggcg
     9541 gaggtgacga tgagcttgag gtcgggccgc ttggcgagga gctggcgcag gtagccgagg
     9601 aggaagtcga tgttgaggct gcgctcgtgg gcctcgtcga tgatcagcgt gtcgtaggcg
     9661 gagagcagcg ggtcgccctg ggtttcggcc agcaggatgc cgtcggtcat gagcttgatg
     9721 tagctctggg ggccgatgcg gtcggtgaag cggatcttgt agccgacggc gctgcccagc
     9781 tcggtcctga gttcctgggc gatgcggctg gcggtggcgc gggcggcgat gcggcgcggc
     9841 tgggtgtggc cgatcagccc ggcgacgccg cggcccagct caagacagat cttgggcagt
     9901 tgggtggtct tgccggagcc ggtctcgccg cagacgatga ccacctggtg gtcgcggatg
     9961 gcggcggcga tctcgtcgcg gcgctggttg acgggcagct cgtcgggaaa gtccgggcgc
    10021 ggacgggcct gctcgcgggc gatgcggcgg gcggcggaat cggcgatgcg caggcgcagg
    10081 gcctcgggca gatcctccag cgtgcggggg cgtcgcggca ggcggcgcag gtcgcggcgc
    10141 agggcgggcc ggtcggccgt caggcagtgt tcgagcaggg agtacggatc ttgccggacg
    10201 gcgtcgggcc ggtcgtgggg tgcagccatg ggtgttcgag ggtgaaacgg agcggatcag
    10261 ccccggacgg cgtggacggt ccacatgccc agggcggtca tggtcaggga gccaaagagg
    10321 tgcaccgccg cggcccccgc cgcccaggca tagcgggctg tggagatcag gtgcacgacc
    10381 tcggcggaga aggacgagaa agtggtgagc ccacccagaa aaccggtcac cacgagcagg
    10441 cgggcttccg gcgggatgtc cgggcgcagg gcgaagaagg ccaccgccac ccccaccaga
    10501 tagcccccga ggagattggc cgccagcgta cccaagggca tctgggccag caccggattg
    10561 agcctcagcc ccagccacca gcgcagccac gcgcccagcg cggcgcccac accgatggcg
    10621 acgagggcaa gggtcgggaa ggaatgactg tggtcgggca tggggccgga ttctaccctg
    10681 ccggaggtcg gaccgggcgg gatcggcctg ggccccgccg cggaggggtc agtccatcgt
    10741 caatacggcc gccccctgca gggcgccgcg acgcagggca tcgagggcct cgttggcccg
    10801 cgccagcggg aagggggtga tctctgcgcg gaccggcacc tggggcgcca gtgcgaggaa
    10861 ttccagcccg tcccggcggg tcaggttggc caccgagcgg atcgtgcgct cgccccacag
    10921 gtcggcgtaa ggaaaggacg ggatgtcgct catgtggatg cctgcacaga tgacgcagcc
    10981 gcccttgtcc acatgccgca gcgccgccgg cacgagggcg ccggccggcg caaagagcag
    11041 cgcggcgtcg aggcgctcgg gcggcggcgt cgcggcatcg cccacccagg tggcgccgag
    11101 ctgacgggcg aaggcctggc tttcgatgtc gccgggacgg gtgaacgcgt agaagggtcg
    11161 ctgctggtga cgcagcacct gggcgacgag atgggccgcc gcaccgaagc cgtacagccc
    11221 caccttctgc gcgtcgtcgg ccatgcgcag ggcacggtag ccgatcagcc cggcgcacag
    11281 cagcggcgcc agcgcggccg cctcgccccc atcgggcagc ggaaagcagt agcgggcgtc
    11341 ggccacggtg tattcggcgt aaccgccggg aatctggtaa cccgtgaagc gcgcctgagc
    11401 gcacaggttc tcgcggccgc tccggcagaa gccacaggtg ccgcaggtcc accccagcca
    11461 gggcacgccg acccgctgac caggcgcgaa gccactcacg ccggcgccga ccacatccac
    11521 ccggccgaca atctcgtggc cggggatcat gggccacacc gggtccggca gctcgccctc
    11581 catcaggtgc agatcggtcc ggcacacgcc gcaggcctcc accttcaggc gcaccgtacc
    11641 gggcggtggc gcggggacgg gcaggcgttc gagctgcagg ggcgtacgag gctggtgcag
    11701 caccatggcg cgcatggtgg cgggggatcc gggcgacatg gagacctcct cgggaccggc
    11761 ggacagtcac gccctcactt taggctgcag agtacacacg gagacttccg cccgtcggaa
    11821 caatctgata aaaacaaact caaacaataa aatcactaaa aacgtaaaat acgaccctaa
    11881 agttcgaata tgaccggtcg ttactggcga cagaacgatc ggagcacacc cgagtgtgcc
    11941 acgctgcaac cggagacgcc atggacagcc ttgccatccc gcccgccctg cactatcagc
    12001 cccgttacga ctgccgtgac ggcgcgctgg tgggcgccaa gctgtacgtg gcccgcccgg
    12061 agcgcagcga caatgtcgtg ctcaccgccc tcgacgacgc ctgcctcgcc cgcctgcaca
    12121 gcgcctgccg gcaggtggcg gactggaacg cccaccacgg actcgacctg atcctgaccc
    12181 tcggcgcgac cctcgaggtc ctcgccaccc ccaccttccg ccaggccctc aacggtctga
    12241 tcctcgacgc ccgcctcgac gcccacagcc tcgagctcaa catcatcacc cgcaccggcg
    12301 agattcccga cgagcaggcc gccgccctgc accacctcaa acgctgcggc gtgcgctttg
    12361 ccatcagtgc cgccagcccc gccaacgccc gcctcgcctg gctgcgccgt ctgccggtgg
    12421 attgcctgga agtccccgcc gcgctggtgc aggacgtgac cgccgatcct gaaggcgtcg
    12481 ccatcatccg cgccctggtg acccgcgccc acaagatggg gcagcgggtc agcgccaagg
    12541 gcatcgccga ccgccacacc gccggcgtgc tggtggccag cgactgcgac ttcgtgcagg
    12601 ggccgctgct gggcggcccg atgacggcgg aggccttcgg agccctgctg gccgccggtg
    12661 aacgcctcaa cccggaactg atccgtggtc cccgcccgga ccgcacgctg ctgctggtgg
    12721 acgacgagga gaacatcctt aacgcgctgc gccgactgct gcggcgggac ggctacaccc
    12781 tgctcaccgc cagcagcggc cagcaggcgc tcgacctcct cgccacccac ccggtggacg
    12841 tgatcgtctc cgaccagcgc atgcccggca tgaccggcgt cgagttcctg cgcaaggcca
    12901 aggacatggt gccggacacc gtgcgcatgg tgttgtccgg ctacaccgaa ctgcagtccg
    12961 tcaccgacgc gatcaacgag ggcagcatct acaagttcct gaccaagccc tgggacgacg
    13021 cgatgctgcg cgccaacatc gacgaggcct tccaccgcaa ggccctggtg gacgagaacc
    13081 tgcgcctgga cgcggagctg caccgggcca acgcggagct ggcgcgcatc aacgaacgcc
    13141 tggccgccct gctcgccgaa cgggagcgtc agctggacgt ggacgagtcg gccctgcacc
    13201 tcacccaggc ggccctcgcg gcgctgcccg cgccggtgct cggcaccgat ccgggcggca
    13261 tgatcgcctt tgccaacgcg gcagctgaca aactctttcc cgcccacgcg cccctcatcg
    13321 ggctgggcgt ggacgaagcc ctgccgcccg acctgcgcga tcttgccctg cgcggcgaag
    13381 gggcaacgga ctaccgggac ggcgcccgcc actttcacgt ggtggctcat cccctccatg
    13441 ccgtgggcac ctgccgcggc accctcttga gcttcacgga caggagtggc gcatgagcga
    13501 agccaccgaa catgtcgaca ccccccccga tggggcagcc gaggaggctg agcgggccgc
    13561 cgagctggaa cgccgccgcg aagcgatccg tgcccgcgtg atgcatctgt tccgccatca
    13621 ggccgacgat cccggcgccc aggcgctgtt gcacgcggtc ctcgcctttc gtcaagagca
    13681 gctgaaatga atccgatgac tgccgccccg accctgaccg acaccctcgc ccgcatccag
    13741 cgcctgccgg tgatgtcggc cgtcgcgatg gaagtcctcc agtccctcaa cggcgccgac
    13801 ctggacgtgg acgcgctggc ccgccgcatc gcccaggacc aggccatcgc cgcgcgggtg
    13861 ctgcgggtgg ccaactctcc gttctatggc atggccgggc gggtggccag catccacgac
    13921 gcgatcatgg tgctgggcct ctctgccgta cgctccctgg tcctggcggc ggccgtggtc
    13981 actgccctgc ccgccggaca ctgcccgggt ttcgatgaac ggcgcttctg gcaccacgtg
    14041 atcggcacgg ctgccgccgc cgaagccatg ggcaaggccc tcaggcgctc acccgggcac
    14101 ctcttcctgg ccggcctgat ccacgacatc ggccgcctcg ccctggtcac cctctacccg
    14161 gccgactacg ccgaggtgct gcgccgggcc ggcgaacggg acgcacagct catcgacgtg
    14221 gagctggaga tcttcggctt cgaccacgcc gaggccggcg ccgccctcgc ccgccgctgg
    14281 aactttccgg acgagatcgc cgatgccctg gcctgccacc acgcaccgga gcgcagccag
    14341 cccggcggac tggccggcgt cctccactac gccgacgcca tcgcccaggc cctcgacctg
    14401 gacggcgaga ccgacagcca gatcccccgc ctgcacgccg ccaccgtcga cgccctgggc
    14461 ctggggcggg acgaccttca tggcgtgctc gccggcaccc gggcgcgttt cgacagttac
    14521 tgcctcctgc tggggtgagc cgatgaatcc acgtccctac gccttgccgg tcagcctctt
    14581 cccgctcgcc gactccctgc cctcgggtct ggtcctgcac cgcagcgacc gcatcctctt
    14641 cgccaaccgg gccatgtcgc ggctcaccgg ttacacggcg gacgagctcg cgcagatgga
    14701 cttctgggcc ttctttccca aggcggccca gggcaaggtg caggaatggg gacgcatgcg
    14761 cctgcagggc gagcatccgc cggcccgcta cgagaccctc atcgtcatca agagcggcga
    14821 ggagcgctgg gtcgaactga ccctctcctg cgaggccatc gacggcgccc gcaccgtgct
    14881 gtgcagctac ctggacatca ccgaccgcaa gcgcgccgaa gcggcccagg cccagtcgcg
    14941 tcagctcctg gcccagatca tcgacggcga cccggtaccg accctggtca tcaacgcccg
    15001 ccacatcgtc acccactgga accaggcctg cgagcagatc accggcatcc gcgccgccga
    15061 catcgtgggc acccgtaagc actggacggc cttctatgac cacgagcggc ccatcatggc
    15121 cgacctcatc gtctccggcg agatcgagac catcacccgg cactacggcc agaagaagct
    15181 gcgccgctcg ccagtggtgg ccagcgccta cgaggccgaa gacttcttcc cccatttcgg
    15241 cgagaacggc cgctggctgt acttcaccgc cgcgccgctg cgcaatgccg ccggggccac
    15301 catcggtgcc atcgaaacgc tgcaggacat caccgagcgg cgcaaggccg aggacgaact
    15361 gcgccagatg cacaccagcc tggaagccaa ggtggcccag cgcaccgtcg aactgcagga
    15421 cgccaaggcc accctggagc gggacattgc ccgccgcgag accgccgagg ccgagttgcg
    15481 cgcccgctac tccgagctga aagacctcaa cagccgcctc tccgacaccc agcagcagct
    15541 cgtgcagtcc gaaaagatgg cctccatcgg ccagctcgcc gccggcgtcg cccacgagat
    15601 caacaacccc atcggctacg tcaattccaa catccgcagc ctcaaaagct acatcgccca
    15661 gttgctgggc gtggtggacg cctaccaggc ccgcgaggcg gccctgccgg aacctgaccg
    15721 cgccgccctc gctgcggcgc gcaaggccgc cgacttcgac tttctgcggg aggacgtcac
    15781 ccagctcctc gccgaatccg aagaaggcac ggagcgggtg cgcaagatcg tccaggacct
    15841 gcgcgacttc tcccgcaccg aggccaccca ggactggcag tcggtggatc tgcgccgtgg
    15901 cctcgacagc accctcaaca tcgccagcaa cgagatcaag taccgcgccg aagtcgtgcg
    15961 tgactacggc gagatgcccc tcgtggaatg cctgccgtcc cagctcaacc aggtcttcat
    16021 gaacctcttc gtgaacgccg cccaggccat gcccgagggc caccgcggcc tcatcaccgt
    16081 gcgcaccggt accgaaccaa ccggcgaggc cgtgtggatc gaagtagccg acaacggcag
    16141 cggcatgccc gccgatgtga tggcgcgcat cttcgacccc ttcttcacca ccaagccggt
    16201 gggcaagggc accggcctgg gcctgtccct gtcctacggc atcatccaga agcaccacgg
    16261 ccacatcggc gtcaccagca cgccgggcgc cggcaccacc ttccgcatcg tcctgcccgt
    16321 gcaccgcgcc ctcaccgacg gagccacccc atgagcgaag ccgccgcccc cgccttcacc
    16381 ctgctgtgcg tggatgacga agccaacatc ctgtcggccc tgcgccgcct cttccgcccc
    16441 cacggctaca ccgtgctcac cgccggcggc ggcgaggaag ggctcgaaat cctcgcccgc
    16501 gagccggtgg acctgatcat ctccgacatg cgcatgccgg tcatggacgg cgcccgcttc
    16561 ctgggcgaag cgcgcaagcg ccacccggac acggtgcgcc tgctcctcac cggctacgcc
    16621 gacatggaat ccaccatcga ggccatcaac gcgggccaga tcagccgcta catttccaag
    16681 ccgtggaacg accaggacgt gctcctcacc gtccgcgaag ccctggaacg caagcagctc
    16741 gaaaaggaga aggcccgcct cgaagccctc acccgcagcc agaacgacga gctcaagtcc
    16801 ctcaacgcca gcctggaaca gaaggtggag gcccgcaccg ccgagctgcg catcgcccac
    16861 gacaagctca agcagaactt cctcacctcg atccgggtgt tctccaacct catcgagctg
    16921 cgcgagggcg ccatgtccgg ccactcccgg cgggtcgccg acctggcccg caagatggcg
    16981 atccacctgg gcttcaacgc caccgacgcc caggacgtga tgctcgccgg cctgctccac
    17041 gacgtgggca agatcggcct gcgcgacgag ctgctgtcca agcccgtgtc ccacatgacc
    17101 ggcgacgagc tgggccagct gcgccgccac ccggtggccg gccagtccgc cctgatggcc
    17161 ctggaaaacc tgcgcaaggc gggcaccctg atccgcgccc accacgaacg ctgggacggc
    17221 cagggctatc cggacggcct ctccggcctg gccatcccca tcggcgcccg cctgctggca
    17281 gtggccaacg acttcgacgg cctgcagatc ggcaccctca cgccccgcaa gctgcgtcag
    17341 gaggaggccc tcgccttcat ccagcagaat cgcggcaagc gttactgccc gcaatcggtg
    17401 gacgccctcg cggcggtcct cggctcgacc gagagtgccc cggcggtggg caacatcgtc
    17461 agcacggcgg cgctcaagcc cggcatggtg ctcgcccgcg atctggtgac gccggaaggc
    17521 gtcatgcttc tcgctgccga cttcgtcctc gacgacggcc tgatccgcca gctgcgcgaa
    17581 ctggaggcct ccgagggccg ccgcctggcg atcccgatcc gcgccgaacg caagacgacc
    17641 gcgtcgatgt aagctttgcc ggtccggtcc ggtcatctcc ggcaagacag cctgccggac
    17701 gctccgacga ccatgatctt ctccttcacc cgcaaacggg aattcgaaca ggtggtgcgc
    17761 gcctacagcg ccgacctgta ccgttttgcc tactggctgt gccgcgaccg cttcgtcgcc
    17821 gaagacctgg tgcaggagag cttcgcccgt gcctggagca actgggacag cctgcgcgac
    17881 aagtccgccc ccaagccctg gctgctcacc atcctgcgca acgagcacgc gcgcctgtac
    17941 gagcgcaagc agttcgacta cgtggacgaa cagccggaag agctggccat cgccgccgac
    18001 cacgacccgg tcgccctgca cgaactggaa gacctgatcg gccgcctgcc cctgtccctg
    18061 cgggaaccct tcctgctcca gactctgggc ggcttttcct gcggcgagat cgccgcccag
    18121 ctggacacca ccgaaggcgc cgtgatggtc cgcctcaccc gcgcccgcca gggcctgcgc
    18181 cagctgctgg acggtgccgc cgccccccgg cacaccggga gtgcctcatg aacgcccaga
    18241 accccgcccg cgacgtcttc gcctcgccca tgaactgcct cgaattccgg cgcctcaagc
    18301 tggccgatcc ccatcgcctg tccgccgccg ccctcgccca ccagcgcgaa tgcgccctgt
    18361 gccaggcctt cgcccgccgc gtcgatacgc aggagcaacg catcgccacg gtcctcgaca
    18421 tcccgctccc cgacgggctg gcagaccgcg tcctggtgcg cgtccaccac ggccggccgc
    18481 gcccctggcg ctttctggct ctggcggcga cggtggtgct gagcgtcggg ctgagcttca
    18541 acctgtggca cgactacccg cgggacgact acgcgcgctt cgccatcgat cacacgctgc
    18601 acgaacccga gtccttcacc gaccaccgcc tggcggatcc gagccagttc cgcctggccc
    18661 tcgcccgctt cggggcggaa ctggaagcac cggtgggcga ggtgttgtac atgaagctgt
    18721 gcccggtccc cggcgggacc ggctggcaca tcgtgatccg caccgagcat gggcaggcga
    18781 ccctgctcct gatcccgcgc aaggccggcg agggcagcgc ccagggtaag ccgctggagg
    18841 ccgaatggaa aggaatgacc gcgctggcgc agccgggcgg cgacgggtat tacgccatcg
    18901 tggcggactc gccggagcgc gcccaggcca ttgccgcgat gatgaaggaa cgcatccgct
    18961 ggcggatctg agcggcgcgc tcaggcgagg atggacgaaa aatcggcccc gtcgcgcagc
    19021 aggcgctgca ggtcgtccac cggcatcggc cgcgcgaaca ggtagccctg gatgaggtgg
    19081 cagccgcgct ggcgcagggc accgagctgg gccgccgtct ccacgccttc ggccaccacg
    19141 tcgagtccca ggctctgggc cagaccgatg acggcttcga cgatcacccc gccctgaccg
    19201 ctgtccagtt cattcacgaa ggagcggtcg atcttgaggg tgtccaccgc caggctgtgc
    19261 aggtaggcca gcgaggaata gccggtgccg aaatcgtcca ccgccacccg cacgcccatc
    19321 tcccgcaggc gcgcaaggat ggtggtcgca tagtccaggt cgcgcatcag caccgtctcg
    19381 gtcagctcga actccagccg ggtcgcgggt acgccgcgat cggccaggag gccattcacc
    19441 atcgggatga gctggacgtc catgaagttc accccggaga ggtttacgct ggcccggaat
    19501 ccctccgggg ccaccggctc caggcgggcc acgtcggcga acacggtgtc catcaggcgc
    19561 cggctcaggg ggaggatcag gccggattcc tcggccaggg agatgaactc cggcggcggg
    19621 atcatgccgc gcagggaatg cttccagcgg atcagggcct cggccttgac gatggcccgc
    19681 cggcgcacgt ccacgatggg ctgatacacg acggtcagtt cgttgttctc gatggcgcgg
    19741 cgcaggtcgc tttccaggct gaagcgcagg aaggccgcca ccttcatctc ctcggagaac
    19801 agcgccacct gctcgccggc ctgggtcttg gcccggttga gggcggtctc ggccgccttc
    19861 agcaaggcgt cggcgtcgct cgcgtggtcg ggcatcatcg ccacaccgat ccgcgccgac
    19921 agggcgatct cctgtcccgg cacatggatc ggcgcccgca ccgtggccag caagccctca
    19981 gccaccccca gcagcatgct gggtgatccg gcctcctgca ccagaatcgc gaactcgtcc
    20041 gacgccaggc gcgccacctt gaggtctggc aagccgccac cgccggcggg ctcgaccggc
    20101 gcgcggtcgc ccatgcattc gcgcagacgg tgggcgatga cctgaagcac ctggtcgccc
    20161 cggtccggcc cgaggctgtc attgatgcgc gcaaaccggt ccacatccag caccatcact
    20221 gcgcagccgg tctcgttgcg gcggcagcgg ctcaccgccg ccagcaggct ttcacggaaa
    20281 aactcccggt tcggcaggcc ggtcagggaa tcgaaatagg ccagctggcg gatgcgccgc
    20341 tgggcctcca cctgctgggt gatgtcctgc acgctgccct ccatcaccca gcgcccccgc
    20401 gccggatcgg aggaaggcgc cggttcggcc gtctcgtgca aagtgcgcac gctgccgtcc
    20461 ggccacacca cccggtaggt gagctgatag ccgtgccccc gtgccgcctc gtcgagggcg
    20521 gccgccacgg tggcccggtc gggcagatag acgtgatcga ccagggcctg cgcgtcatcg
    20581 ccgaagctcg ccgcggtctg cccgaagagc cggaaatact gttcggagcg ctggatgctg
    20641 ccctgctcgg gccaccattc ccaactgccc aggcgggcca cgcgctgggc gttggagagg
    20701 ctttccttgt tgcgcaccag ctccttcatc acgtcggcgg agcggagcac gtaacgcacc
    20761 cggaagcgca gcagggtcca gttgatgggt ttggagatga agtcggtggc gcccgcatcg
    20821 aaggcgcgct gcacggactc gctgtcatcg agccccgtca gcatgatgat ggggaccagc
    20881 aggccggcat ccagtgcgcg cagacgggca cacacctcga aaccgccgag tcccggcatc
    20941 accacatcga gcaacaccag atcgggccgc cgcgcggaga aaagctcaag cgcctgctgc
    21001 ccgtcctcgg cctcctgcac ggcgtacccc ccctcgcgca gggcttcgcc cgccatcagg
    21061 cgtgtcagcg ggtcgtcgtc gacgatcagg atgcgggcac gggacgcttc agtcatggtc
    21121 ttggggcgcg gtcggcgccg gagaattcgg gggtgtatcc ggggacggcg ggacggcggc
    21181 gcgctccgcc tcacccgctg caagcagccc gcgggcgacc atcaccgccc ggcaggcctg
    21241 ccaggcttcc gccagggcct cgacctcgcc ggccgccggc acgccgcctt cgcggatgtg
    21301 atcatccagg gcacgggcac atcctgccag acggacggcc ccgagggcca gacacccgga
    21361 cttcatcttg tgggccacgg cccgcatcgc gctccagtcg cctgcctcga aactactgcg
    21421 cacgctggcc acgtgccgtt cggtttcggt ggcgaagagg tccaccaggc ggaccagcat
    21481 gggcgacgac ggattgcggt tgacgcccgg cagcccgagc aacacatcca tgtccagcgc
    21541 cgccggcgcc tccacatggc cggacggagc cggtcgcggc gatgccagca gggacggcgc
    21601 gggctcgacc ggcacgaagg tggtgagcca tttggccagc gtttcgccca gttgttcccg
    21661 cgacacgggt ttgaccaggt agtcatccat gcccgccgcc aggcaggcgt cccggtcttc
    21721 ccgcagggcg ttggcggtga gcgccaccac cggcacccgc ggcagacagc ggaaatcctc
    21781 gtccgcgcgg atcgccacgg tcgcctggcg accgtccatc tcgggcatct ggatgtccat
    21841 cagcaccagg tcgaagcgct cgcggcggca ggcctcgaag gcctcgcggc cgttctcggc
    21901 gacggccacc cggcaaccga gggattccag caccgccacg gcgatttccc ggttcaccgg
    21961 atgatcttcg gccaccagca cgcgggcggt cagcggcacc acggacgtgt cgtccgggtc
    22021 ccgctcctgc ccgaggaggg cacgccggat cgccaccccg gtgagcggac cggccagcac
    22081 ctcggcggcg ccggcgatgc gggcctgctc ggcctcctcc cggcctgttc ctaccacagc
    22141 cagacgcggc ggcgcagacg gcgcctcccg caacatctcg agcaacgcaa agcccttgcc
    22201 accgtcgaag cctgcgtcgg tgacgatcca ctggaacacg gcaccggagg ccagccgggc
    22261 cgtcacggcc gcctggtcgg gcgcgatctc gacggcacct cccatgaaga cgatctgttc
    22321 ggcaagcgcc tcccgcgagg ccgggtcgcc catcgccagc agggcccggc gaccgtcgag
    22381 caccgcgtgg gcgtcgccct gggtcacggc gcccagctcg atctccaccc agaatgtggc
    22441 gccctcgccc ggcgcggaat cgtagccgat gtcaccatcc atcagggtca ccagctgctt
    22501 ggcgatggac agccccagcc cggtgccgcc gaagcggcgg gccatggaat tgtcggcctg
    22561 ggaaaaggac tggaacaggc gcgattccat gccccgcggc acgccgatgc cggtgtcgcg
    22621 cacggtggcg cggatgcgca cccgaccggg gtgggcgcag ggcagccggc gcagctggat
    22681 ggcgatgctg ccgtgctcgg tgaatttctg ggcgttggaa accaggttgc tgatcacctg
    22741 acggacccgc agggcgtcgc cgcgcaccat gtccggcaca tcctgttcga ccttcaggcg
    22801 caggcgaatg ccgcgctgct gggcagaggt cgcgaagagg gaggccacct ggtccacggt
    22861 gtcgcgcagg gaaaacgcca gagactccag ctccaggcgg ccggcttcga tcttggagac
    22921 gtccagcaca tcgttgatga cctccatcag ggcccgcgcc gagtcgtcca gcatgtccac
    22981 gaaatggccc tggcgcgccg ccagggaggt ctggcgcaac aggccgatca tgcccatcac
    23041 gccgttcatg ggcgtgcgga tctcgtggct catgttggcc aggaactggg acttggcccg
    23101 gttggccgct tcggcctgtt ccttggcagc caccagctcg cgggtacgca ccgccacctc
    23161 ggattccagc tgcgcccgat gcagggtcag ttcctggtca cgggtggcga tctcggccag
    23221 catgccgttg aagccggcga tcagctcacc cacctcatcc gcgtagccgg cgggcgcccg
    23281 caggtcatag cgcttgtagc ggcccacctc gcgcatgacc tccgccaggc gctggatcgg
    23341 ctcgacaatc atcgcctgca tgcgacgggt cagcagggag gccgccagca aggcaccgcc
    23401 cagaccgaac agggcataac cgaaccaggc caggaattgg ccgaggaccg ggtgcaggtc
    23461 gaattcgatg atcagccggc ccaggttctc cccatcgctc tcgatgccct gggcgaccac
    23521 cagatgggtc agcccccacc ccaagcggcc cggctcggcg gcggccttga gcgcctccac
    23581 ccgcgcctgc gcggcgccga caccggcagg gtaggcggcg aagacacggc cgtccggtcg
    23641 taccagcagg gccgtcgcga cccgctcatg gtgacgcagg gaggcaagca ggctgtccgc
    23701 cacacgggga tcgtcgaagc ggagcggcga cgcggcaccg tcggcgatga tctccgagag
    23761 cacgcgcccc tcgttattca cgcgccggat ctcgttgtgg atctcggtca caaagagggc
    23821 caggaacccg acccccagcg cgatgagcac ccccagacgt acgacggtgc tcagtttgcg
    23881 gccgatgggc agatcccgca gggcgttcag cggattcatc tcacagcccc ttcagcccga
    23941 agacgttgcg tcccaggcgc agcagctgcg agctggcctt gagggtgtcg cgggtcagcg
    24001 cggcctgatt gatgtcgaaa cggacgcgat cctcctcggt caccaggccg attccgcctc
    24061 ccgcctcggc aaagccgtcg atatcgctca cggtcagcac gccacgcccg gccagggtgc
    24121 gcaggatcag gccggtgcgg cgctcttccg actcggcgac gaacaccacg tggcagtcca
    24181 ccgcctgctc cagactgtcc gcctggcgga cccgcacctc atgaccctgc accaggcggc
    24241 cgtcgatgcc gtgcagcgcc ccgtcgaaag gatcccgccc gagcaggcag acgcgcagca
    24301 ccgccccgcc gggcaggcgc gccgccggcc actccacgta gcgggcgaag ttgaggatgt
    24361 aggccgcctt gatctggtac tcgccgggcg gtacggcgac cgccggcagg ccggccccga
    24421 gggcggccca cagcaccaac aggaagcgca gtatgcggaa cgtcacgggg gcggcctcag
    24481 taacgccaca caccctggac aaagccccgc cgcggcagat acgccggcac gccggggaac
    24541 acgtcggaga cgtactccag gtgcaggggg tcgaacaggt tctgacccac cacggcaagc
    24601 tcgaacgtcc ggttcagctt ccacgcatag cgcaggtcca ccgcggtgta ggccgggatg
    24661 tcgccctcac ccacgcgccc cacgcggcgc agcatcaggt ccacatcgcg cccgccgccc
    24721 agggcgaaca ccccgtgcac actcgcccag tgattggggg cgctgtactt caggttgccg
    24781 ccaaaggtgg ccagcgccac gtccgatgcg gggtcgatcg aggtgttcat gagggtgtag
    24841 ctggcctgca gacggaacca ggctgtcacc ggcacatcca tgcccagttc cagcccggag
    24901 ctgcaaccgg acacgttgtt gctgcgcagc acgcgctgga tcggcagata gcctggcggc
    24961 acgccgatgg caatgcccgg cacgatcagc cccgccggat ccgtggcgcc gccaatcaga
    25021 ccgctgtagc gctggtggaa gagcaccgcc tcgacactcc cgttgcccag attgcgccga
    25081 tagccggtct ccacggcaac cacccgctct gcgcgacggt tccagtcggc ggccggctgg
    25141 gagacgatgg ccgcggacgt cgggccgaat cccggcggca gcggcacgat gcgattgacc
    25201 agattcgcgt cggtctcgcc gatggacggc gtgcgcgcgg cccgcgacca gcccgcccac
    25261 acactgtcgg tggtggtcgg cgtccacatc aggcgcacgg tgggttgagg ctcgaagccc
    25321 gacaggctgc tgtactcgaa acgccccccc agggtcagca tcaaccttcg cggcagcaaa
    25381 gtaatctcgt cctgggcaaa cgcgctggcg atatggctgt cgcggcggtt gggcgtcatc
    25441 cacagcatcg gcgtgcggga ttcgatctcg gcgcggaacg cccggtaacc cagcccccac
    25501 atcagggcgt gtttgcctac gggtgccagc cggtgctgca ggtccagatc ggcggtcgtg
    25561 accgcgccgg tgccgtagct gcccacgtcg gcctgctgtc gatccagata ggcctgcagc
    25621 gtcgcctcgc cgccggccag cccccagttg taacgtccca aaagattggc gccgtcgaac
    25681 tgcggggcga aggcattgcc ggacggcgct tccgccacca gccccacggc cgaatcaccg
    25741 ccgctgcgcc ggtaggcgtt gccctgcagg gaccaggtgt tgcggccccc cacggccgaa
    25801 tccatgcgga atccggccag ccagcctgtc agcttgtcac ccaccgcgtc gccgtcctca
    25861 cgccgcgtcg aggagatctc gccgcccttg gcgaagaccc gcagggccgc gccgtttgcc
    25921 agggtgaaac cttgccgcgc cgcgacaacc ggcgcaccgc gatcgtccac ggtcacggag
    25981 gccagcgtgc cctgggtggc cgtcgccttg cgggtgacga tgttgatgat gccgttcacc
    26041 gcattggcgc cccacaggga cgcgccgggg ccgcggacca cctcgatgcg ctccacgtcc
    26101 tcgagcagca cgtcgatggc ttcccagaac acgccggaaa acagggggct gtagacgctg
    26161 cgcccgtcga tctgcaccag cagcttgttg gagaagcgcc cattgaagcc gcgcacgctc
    26221 accgcccagc gtgccgcccc gatctgcgcc acattgacgc cgggcacctc gcgcaaggcc
    26281 tcggggatgc tgcgggcgcc gctgcggcgg atatcgtcgg cggacaggac cgtcacggcc
    26341 gcaggcgtgt ccgacaactt ctgggccttg cgggcgacgg acgccacttc cagcttgacc
    26401 agctcttcca ggctcagatc atcgagctcg cgttcaccgg catggaccgg cagggcaagg
    26461 atggcactca gaacggcaat actcaggacg gtgggattca tggacaagga ggacagtgcg
    26521 ggcgacacgt tcaagacgac ggaaagcggg acaacttgag cggaatttca cccgatgcaa
    26581 ggtcgatacg accctcgaat atttctcaaa tccgccaacg ttaactaatt cgtcaataca
    26641 cacgccaatt ttgtcacact gtttcccgcc tcacaaaaat gtgaaattca gggcggaacg
    26701 catcgcgacc gaaccgggcc ggcaacccga cgttcggcta caatcgagca tttacaatgc
    26761 aataagggcg tccagacggc catcgccgcg cccacccaag gtaagcccat gaccgcaccg
    26821 accccccgcg gcgaagacaa cgccgccagc aacttcatcc gcaacatcgt tgacgagcac
    26881 cgtcgcaccg gcaaatggag cgggcgtgtc gaaacgcgct tcccgcccga gcccaacggc
    26941 tacctgcact acggccacgc caagagcatc tgcctcaact tcggcctggc cctcagctac
    27001 agcggcgcct gccacctgcg cttcgacgac accaacccca ccaaggaaga gcaggaatac
    27061 gtggacgcca tcatcgaggc cgtccagtgg ctgggcttcg actggggcga gcacctgtac
    27121 ttcgcgtcgg actacttcga caagatgtac gccatggccg aatacctgat caccgtgggc
    27181 aaggcctacg tggactccct gtccgccgaa gagatgcgcg cctaccgggg caccctctcc
    27241 gcgccgggcc aggacagccc gtatcgaaac cgcagcgtgg ccgagaacct ggaactcttc
    27301 cagcgcatgc gcgccggcga gttccccgac ggcacccaca tcctgcgtgc ccgcatcgac
    27361 atggcctcgc ccaacatcaa cctgcgcgac ccggccatct accgcatcc
//
LOCUS       DAOQMM010000043        26303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_562_length_26303_cov_50.196434, whole genome shotgun sequence.
ACCESSION   DAOQMM010000043 DAOQMM010000000
VERSION     DAOQMM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 26303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26303
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_26303_cov_50.196434"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLH85_08935"
     CDS             <1..681
                     /locus_tag="PLH85_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNB64726.1"
                     /translation="AAGRGERMRPLTDTCPKPLLAAGGKPLIVWHIERLATAGITELV
                     INHAHLGAMIEAALGDGRRLGVTIHYSPEGSALETAGGIARALPLLGDAPFLVINGDV
                     FCDADFAPLARAAAGLRADGPLAHLLLVPNPPHHPDGDFGLHADGHIRDDGSGTRLTF
                     AGVGAYHPALFAGVDPDRPAKLAPLLRTAMSDGRVRGTRFDGRWVDVGTPARLRELDQ
                     ALRTGTPD"
     gene            749..2065
                     /locus_tag="PLH85_08940"
     CDS             749..2065
                     /locus_tag="PLH85_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNB64727.1"
                     /translation="MSLDITPFQARRQRLLATLQASGGGVAILPTAPERLRNRDAHYG
                     YRFDSYFYYLTGFREPEAVLVLVAGDTPRSLLFCRNKDVEREIWDGFRFGPEAARDSF
                     GFDEAYAIGELDDQLARLLADQPAIFHSVGYEPDWDSRIAAALNRVRAQARSGARAPE
                     QIRDIRSLIDEMRLVKDDAELALMRRAADISGAAHRRAMRTARPGTREYEVEAELLHE
                     FRRGGSQAPAYTSIVASGANACVLHYVENDRVMQNGELLLIDAGCELDGYASDITRSF
                     PVNGRFSGPQKAAYELVLAAQAAAIAEIRPGASWNAGHDAAVRVLAQGFIDLGLLAGS
                     LDGVLETGSYRQFYMHRTGHWLGLDVHDAGDYKLAGEWRPLVAGNVLTVEPGCYIRPA
                     DGVPEAFWNIGIRIEDDALVTPDGCEILTTAAPKSVADIEALMADR"
     gene            2067..3209
                     /locus_tag="PLH85_08945"
     CDS             2067..3209
                     /locus_tag="PLH85_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNB64728.1"
                     /translation="MDRHDLVIVGAGPVGMALALALKDAGLDILLVDARARGAARHDP
                     RVLALAHGTRLTLERLGVWTQLRATPIETIHVSQQGGFGRSLLRATDYDQPALGYVVA
                     AGDLAAALDDALLAARIPVRGDASVSGLAAGADDVLVSLADGHTLSARLAACAEGAIQ
                     GDEDDTLVERDYGQHAVISVATPAGSHGHRAWERFTPQGPLAVLPCGHDCAIVHTAAP
                     DAADALMTLDDAAYLAHLQAHFGQRLRFAAVGPRARYPLRLRYRPNPVGHRTVWLGNA
                     AQTLHPVAGQGYNLALRDVWACAQVLLRSGGDPGAPETLATYARERRLDRAGTIRFTD
                     TLVRLFSTDLAPLRHARGAGLFALDLLPPARHFVAKRMMFGARAWP"
     gene            3330..4772
                     /gene="mgtE"
                     /locus_tag="PLH85_08950"
     CDS             3330..4772
                     /gene="mgtE"
                     /locus_tag="PLH85_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNB64729.1"
                     /translation="MSETDDLQHHDDLDRHVREVQHLLARHKLVEDLVHRQDMPRHDL
                     VENLVHKQHVNELTHKLDSLHPADIAHILEALPLDERLFVWDLVKAERDGEILLEVSD
                     AVRESLIESMDTAALQAAAEQLDADELADLAPDLPHEVMEAVYQGLAQEEREQLRAAM
                     SYPEDSVGALMDFEMVTVREDVTLEVVLRYLRRFEALPDHTDQLFVVDRDERLRGVLP
                     LNRLLLNDPDVTVSDVMLTDTLTLEPADEAETAAQAFERYDLVSAPVVDYHRKLVGRV
                     TVAAVVDFIREETENELLSQAGLKEGEDIFAPVWDSVKNRWSWLAVNLVTAFIASRVI
                     GAFENSIEKLVALAALMPIVAGIGGNSGNQTITMIVRAIAMGQVQHDALPRLLKKELG
                     VALLNGLVWGGLLGVLAWILYGSGKLGAVMTAAMTLNLLVAAFVGVMAPMIRHRFGQD
                     PAVGSSVIITAVTDSGGFFIFLGLATIFLM"
     gene            4877..5596
                     /locus_tag="PLH85_08955"
     CDS             4877..5596
                     /locus_tag="PLH85_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HNB64730.1"
                     /translation="MKLAATLATLALSFNAGAAEFNAPWNDAQIALVIDPYAANSLKW
                     DALAGEPRVVAIIHKATIGTQGLDKAYWTRKAEARQRGYLWGSYHWGVAGDPEKQADY
                     YLDTVKPGDDELIALDLEDATSKTLMSAAEAVRFIERIHARSGRYPVLYTNHASAVLI
                     SKTYKNSVFAHTPLWYARFKTQVTDFPKGLWPTYTLWQFSSEIKPQKAIPGTRSDMDV
                     NVFNGSVEQLRTAWPLTQRAP"
     gene            complement(5624..6910)
                     /gene="hemL"
                     /locus_tag="PLH85_08960"
     CDS             complement(5624..6910)
                     /gene="hemL"
                     /locus_tag="PLH85_08960"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNB64731.1"
                     /translation="MTSRNEALFARAQRTIPGGVNSPVRAFRSVGGTPRFLQRAQGAR
                     VWDADGKEYLDYVGSWGPAILGHADPVVVEAVRAAALDGLSFGAPTEREIEMAERLCE
                     LLPSLEMVRLVSSGTEATMSAIRLARGFTGRDAIVKFEGCYHGHADSLLVKAGSGLLT
                     FGNPSSAGVPEDFAKHTVVLDYNDPAQLEETFKARGNEIACVIIEPIAGNMNLVKPSA
                     EFMHLLRRLCTEHGAVLIFDEVMTGFRVGPRGVQGLFGITPDLTTLGKVIGGGMPVGA
                     FGGRRDIMEKIAPLGAVYQAGTLSGSPVAVAAGLASLNQIARPGFYDGLTARTLRLVQ
                     GLAAAAAQHGVRFTADAVGGMFGLYFAPAVPTSYADVMASDRETFNRFFHAMLDAGHY
                     FAPSAFEAGFVSAAHTDADIDSTIATAAAWFAANGG"
     gene            complement(6928..7560)
                     /gene="thiE"
                     /locus_tag="PLH85_08965"
     CDS             complement(6928..7560)
                     /gene="thiE"
                     /locus_tag="PLH85_08965"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNB64732.1"
                     /translation="MAKTDPVLRRGLYLVTPDWTDTGRLVAMLPALLATRPALLQYRN
                     KLADAALRRVQVAGLLPLCRAAGVPLVINDDLALALECGADGVHLGRDDGDLAAARAA
                     LGPHRLLGVSCYAEWPRAEAAAAAGADYLAFGAMFPSSTKPSAPPAPLALLGRAKAAF
                     GRPVAAIGGITVDNAPLLRAAGADLLAVISDVFSAPDPAARAAAYGRLFD"
     gene            complement(7547..8386)
                     /locus_tag="PLH85_08970"
     CDS             complement(7547..8386)
                     /locus_tag="PLH85_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNB64733.1"
                     /translation="MISTRSDPSPPLVMVFAASDPTGGAGIQADIMTLSGMGCHALTV
                     ITALTVQDTAGVEAVLPTDAEFVDEQARALLEDMPVQAFKLGVLGSLDNIEVIARILA
                     DYPHVPVVVDPVIASGRGDELASESMLEGLCELILPQATVVTPNTLEARRLAARHRDD
                     DDTPTLAECARRMVAMGCEYVLITGSHEHSVQVINTLYGRGGVVRADTWERLPGAYHG
                     SGCTLASAVAAALARGLEVPVAVRDAQEFTWQTLAYAFRPGMGQHIPDRFFWARPVDD
                     GED"
     gene            8502..8654
                     /locus_tag="PLH85_08975"
     CDS             8502..8654
                     /locus_tag="PLH85_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNB64734.1"
                     /translation="MCLICGYIYDEAAGAPNEGIAPGTRWEDIPPNWTCPECGARKDD
                     FEMVAI"
     gene            8718..9113
                     /locus_tag="PLH85_08980"
     CDS             8718..9113
                     /locus_tag="PLH85_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64735.1"
                     /translation="MNLNGIKVMVIDDSNTIRRSAEIFLAQAGCHVVLAEDGFDALAK
                     IADHAPDLIFVDIMMPRLDGYQTCSLIKKNARLKSTPVIMLSSKDGLFDRARGRLAGS
                     DEYLTKPFTKESLLRSVASHVPPRAGAAN"
     gene            9135..9500
                     /locus_tag="PLH85_08985"
     CDS             9135..9500
                     /locus_tag="PLH85_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64736.1"
                     /translation="MPIKKILVVDDSPTERLALSEVLVRHGYQVVTADSGDEAVIKSK
                     TEQPDLILMDVVMPGTNGYQATRTIVRDEQTRNIPVIICTSKGLETDKIWGMRQGAFD
                     YLVKPLDHAMLLDKIKALG"
     gene            9508..10023
                     /locus_tag="PLH85_08990"
     CDS             9508..10023
                     /locus_tag="PLH85_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB64737.1"
                     /translation="MAKRISLQEFQAGLARRLAEARDTRASALLAVQAGSRNWLLDLA
                     EAGEILAVPPLAPVPLVHPWFRGLASVRGNLFGVVDFAAFHGEAPVVTGGQARLLLVG
                     ARLNLNCALLVSRALGLRSVDDFEPLSEPEDATTPWIGAQLQDAHGHTWTRLRVQGLL
                     NHSRFLDAALT"
     gene            10110..12194
                     /locus_tag="PLH85_08995"
     CDS             10110..12194
                     /locus_tag="PLH85_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB64738.1"
                     /translation="MATNTGDNTILEASERGEVSAVLPFLAGKSTAEQLRLLRLPFIA
                     FAVLTAVLVIYQIRVSSFGTAYVTAAGQMRTLSQQMAKASQLALQGEAGAFAELKRSR
                     EQFNHLLEAVTQGGDVDGTGVPGGSSAVADELTAVTELWQKSDKDAGQLLGQEKNLAS
                     LGNAVATINTKNAHLLELTEQVAALKLQTGASAREIATANQVVMLTQRIAKNANALLV
                     ANAIDPEVAFLLGKDTNTFREQIEALMKMTAAGSDAQSKLVELEGVAKDNLAAVQAIL
                     GNIQLLVQAKQSGSNIFQNSGPLLEKTSALAEGYSRAYTGFFTWVTVGIMLFAVLSIV
                     LLLLMAKVYNDDIARRRFSAERLRESAESERNSTQQAILRLMNEMGDLADGDLTVRAT
                     VTEDITGAIADSVNYTIEELSVLVRRINDAASRVTSATESAQRTSDELLAASERQAQE
                     LRLAGDTAHSMAVSMSEASEGALQSAQVARRALEAAQKGATAVEDTIKGMNDIRDKIQ
                     DTSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEV
                     QRLAERSAEATKQIGAIVKTIQTDTADAVAAMENATRDVVDGATLSETAGRELAEIER
                     ISAETAELIERISVSTEIQAKSATQVAEKMKGILAVTDRTTAGTQQTAVSIGELADLA
                     VELKGSVSGFKV"
     gene            12238..17895
                     /locus_tag="PLH85_09000"
     CDS             12238..17895
                     /locus_tag="PLH85_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB64739.1"
                     /translation="MSSAHELDLGPLTWVKGEIDLALERAAGALAEAETASDRLGRIK
                     FAQTHLHQAHGALSIVGLDGLTQFSEALDRLLGELAAEQVTYTPAIARLATRGLAALG
                     NYLNELTRGQPDQPLRLFELYAQLVEARGHEAPGPSDLFYPDLSLRPAFAPLPEGGTD
                     DARTLRALRGRFERGLLKWLRNPGDASGPAEMRDAVNGIERTQRQPASRAFWLASAAF
                     FDALGQQAIDVDPPLKRLCGRIDAQMRRLLEGSAVVAERLMRDVLYQVAIARCNTPPV
                     NTLREAYRLVNLIPAPGRSVSDAPVAPLLVALHSGLGDAREAWTLFTGGGAIGLPRFQ
                     DALAPLPATARAIGQPHTAALIEGLGRLAQWLRQDPLRLTDTLSMDMATAMLLVEGLV
                     DSHEMPSPGPRLKKQIAAVTERIQAYLRGEQPAPLSIADPDTADQQLGRQLAHEILAN
                     LGDVEQHLDGFFREPARTDLLAATSAPLKQVEGALAIMNQGAAVRLVQDCAAAISALE
                     SGRQSPEAAIFEDIAHRLSALGVFIDALQHGPADLEQILNPSTGDSAGDSLEAELVQQ
                     KRETAALAAALQENPDDIGLRTELLLNLEGLRQDASLTADAELEKQAREALNLLDAGA
                     PFAAPPAIVPEAMAPALSPEAARLLDASDAEIDAELLDIFIEEASTVRDTIASHLDLL
                     IAAPGDQEHLTTIRRSFHTLKGSGRMVGLKDFGEAAWGMEQTLNHWLRLERPATPELL
                     ALIRRAHVAVSDWVDQLQAGGGLSLDVADILREAERLRGEEDHGAALEIVQNLESSIL
                     QPIIELDAPPPAPAVEFPALDFDSLEASGERPSTDRLDIVLDDAPAEPPVALEELVWA
                     EPPAVPAPPAPTAAEPAPSELATDAIEIEIEIEAAALEDDDVFPEEGAEVIDLGAPEP
                     LEPDASEAVDLPESDATAEAIELGAAAPESVADAPSHGREHDTEDIDSLEDDDAVRLG
                     HVVLSRPLFDLYLAEARQHLATLRHELGRMAVNPSLLPANAAIRAAHTLSGISGTAQF
                     TAPQSLAKALELAQQRLADHHQAPSADQAQLLNGAATALETMLAQLSGRRLPDAQPEL
                     VDALDNVLRGHLAEVITLRPEIPSDLPPPELATAPLARSPEPVTPPPEAPSAAAPVLR
                     DELDAELLPIFLDEGNELLHSLDTALRAWRAQPDDATQPAAVARLLHTLKGSARMVGA
                     MSFGASIHELETRLERARRLAPDDIAIVDDIESALDRSRQTLETLGISPPATTPAAVA
                     AAPIDVETGAVEAVPAGADGTLAAPRAQLRVEATRVDQFVNEAGEISIARTRIEGELR
                     TLRRSLLDLTENVIRLRNQLREVEIQADTQMQSRLSQVETRHTEFDPLEMDRYTRLQE
                     LTRMMAESVGDVTTVQQNLLRNLDGAESALHAQSRMTRDLQQSLMHVRMVPFDSLAER
                     LYRLVRQTAKDVGRRANLDIIGGQIEIDRGVLEAMTGPLDHLVRNAVAHGIELPDARR
                     AAGKAEIGQITLRITHEGNEVVIEFRDDGAGLDFDAIRAHAIATGLLGTDEESDDKRL
                     ANLIFLPGFSTATLSSVAGRGVGMDVVKSETAAVGGRLSVETDRGRGTTFRIHLPLTL
                     AVTQALLVRSGGRTWAIPSSMVAQVLELKAAALEKVRAEHGTDWLGERFAYRYLPRLL
                     GDSATQPQVERFNWVLLLRAGSETLALHVDALRGNQEVVVKNAGPQYARMVGFSGATV
                     LGDGEIVLILNPVALAARAPDATDDGVPGLPAAPAIPEVPLVMVVDDSLTVRKITGRL
                     LEREGYRVLTAKDGVDALEQLGHNDPDVIIADIEMPRMDGFDLTRNIRADARHKRVPI
                     IMITSRMAEKHQRYAEEIGVNHYLGKPYDEDLLLRLIRRYTRA"
     gene            17973..19931
                     /locus_tag="PLH85_09005"
     CDS             17973..19931
                     /locus_tag="PLH85_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNB64740.1"
                     /translation="MNLLFEEDGAFKTGTVLTDNDSTLQVETPHGKRLKLKSSHVLLR
                     FPQPAAGELLDRAEIEAEGLDTEFLWDVCGDDEFGFADFAREYYGHAPTPIEATAVLL
                     RLHAAPIYFHRKGRGRFRKAPADILQAALAGLEKKRQQAAAIERMRDALLAGTLPEEF
                     RPLIRPLLFKPDRNRLETKALEAACAECGKSAARLLLDCGALASSQELHFDRFLYEYF
                     PEGTAFPSVDAPTAPADLPLAPVRAFSIDDAATTEIDDAFSVTATPDGWRIGIHIAAP
                     GLGFGRDSALDAIARTRLSTVYMPGHKITMLPDVVVEGFTLGEGRDCPALSLYLELAR
                     DLRITGHHSALERVPVAANLRHHDLEPVFNETTLADGGPDFPWKAELTLLWELATVLE
                     AGRGKPAANQTLVDYAFNVDWSVATPDGPGRVDIGRRPRGSPLDKLVAELMICANSTW
                     GKALADAGVPALYRAQTGGKVRMTTSAAPHEGLGVDCYAWSSSPLRRYVDLVNQWQLI
                     AWLRGEAPAFPPKSADLIAAMRDFELTYAAYADFQRGMERYWCVRWLRQIERPELTAR
                     VLRESLVRLEDIPLTFKLPSMPTLPSGTRVRLAIDSTDLIDVEVRATYLETLADAPLE
                     MAEALAEDVTDAPLPEAAPPASDAPPAS"
     gene            19995..20894
                     /locus_tag="PLH85_09010"
     CDS             19995..20894
                     /locus_tag="PLH85_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNB64741.1"
                     /translation="MAAGRLPLVAPSRKASRYALEWGVGLSLLLHALLLSVHFVMPDA
                     NHFTRTHQDLAVVLVNARHAQRPDKAELLAQTTLDAGGTSTPDARASTPLPPQTLSRA
                     GDGLVEAQRRAETPQTPQMQAMTAPSGKTALAVDSRQTAPTEPPRQVSGYDLLDSSAA
                     VARIEAQIDKDLVDYAARPRKKFIGARTQEYRFAQYVEDWRQKIERVGTLNYPEAARG
                     KVYGSLLLSVTIRSDGTVVSIDVHRSSGHKVLDDAARRIVQLAAPYAIFPPDIRRDTD
                     VIEITRTWTFTNSDMLQTSDKKE"
     gene            20900..21730
                     /gene="aroE"
                     /locus_tag="PLH85_09015"
     CDS             20900..21730
                     /gene="aroE"
                     /locus_tag="PLH85_09015"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNB64742.1"
                     /translation="MDRYAVIGNPIAHSKSPRIHALFAAQTAQALSYEALLGPLGAFA
                     ATVDAFRAAGGRGLNVTVPFKLDAFAYATRRTPRAEAAGAVNTLAFGPDEVLGDNTDG
                     AGLLRDITVNLGCPLTERRVLLLGAGGAARGSLLPLLLTEPARLVVANRSSAKAVALA
                     AEFARLPSCAVAVKLEGCGFADLAGAQFDVVINATSASLGDAAPDLPAGLYAPASLAY
                     DMMYGKGDTPFLAAARAQGAGRLADGLGMLVEQAAESFALWRGVRPDTAPVLAALRAG
                     "
     gene            21753..22445
                     /gene="mtgA"
                     /locus_tag="PLH85_09020"
     CDS             21753..22445
                     /gene="mtgA"
                     /locus_tag="PLH85_09020"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNB64743.1"
                     /translation="MKTFGRWLKRGLLGLVLVFLAWHLWLLGHVLWWKWQPPSETSFM
                     SLRLDELATRRPDAELRYRWVPYERISVYLKRAVVAAEDDKFTDHEGFDWDGIQKAIE
                     KNQKKGRAVAGGSTISQQLAKNLFLSPARSYLRKAEEALITVMIETFWDKRRILEVYL
                     NVVEWGDGIFGCEAAAQRYFHISAAQLGPAQAARLAVMLPNPRKYEKDFGPRLAAHAA
                     RIQARMQYSDVP"
     gene            22535..23563
                     /gene="dusB"
                     /locus_tag="PLH85_09025"
     CDS             22535..23563
                     /gene="dusB"
                     /locus_tag="PLH85_09025"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNB64744.1"
                     /translation="MQFAGFTLRNNLFVAPMAGVTDRPFRQLCKKMGAGLAVSEMVTS
                     NSLLYGSAKTQRRANHDGETDPISVQIAGADPAMMAEAARHNIDRGAQIIDINMGCPA
                     KKVCNVMAGSALMQDEALVARILEAVVGAAGATPVTLKFRTGWSKAHKNAATIARIAE
                     TSGVQLIAIHGRTRACQYTGDAEYDTIADVKTRVRIPVIANGDITTPHKARFVLDYTG
                     ADGVMIGRAAQGRPWIFREIEHFLSTGEELPPPLVSEIHQVCRDHLADLYAFYGEDTG
                     VKVARKHISWYTKGLAGSAQFRRVMNTLPTVQEQLTAMDEFFDRLADRDSRLVYVDPE
                     DFPKELAA"
     gene            23560..23799
                     /locus_tag="PLH85_09030"
     CDS             23560..23799
                     /locus_tag="PLH85_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB64745.1"
                     /translation="MSRSSNDIAESVHRALETYFRDLDGEKPNALYDMVIRNVERPML
                     EFVLQQAGGNQTLCAEMLGINRNTLRRKLSEYQLL"
     gene            23852..25426
                     /gene="purH"
                     /locus_tag="PLH85_09035"
     CDS             23852..25426
                     /gene="purH"
                     /locus_tag="PLH85_09035"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNB64746.1"
                     /translation="MKVTQALLSVSDKRGVVEFARELSALGIQLLSTGGTAKSLRDAG
                     LPVTDVSDYTGFPEMLDGRVKTLHPKVHGGILARRDLPEHLAKLEAHSIPRIDLVVVN
                     LYPFQATVAKPDCTLEDAIENIDIGGPTMVRAAAKNHGNEAGGVGIVTDPEDYAAIVA
                     ELQANAGALSYKTRFDLARKAFTHTARYDSAISNHLTALAEDGSKQAYPERFQLAFDK
                     VQDLRYGENPHQSAAFYKESDAPAGAIAAYTQVQGKELSYNNIADADAAWECVKAFDA
                     ATPACVIVKHANPCGVAVAASPLDAYKKAFSTDPTSAFGGIIAFNTVVDKAAAEAVSA
                     QFLEVLIAPAYTEEALALLKAKQNVRVLIVPLGAVRQTDYKRVGGGLLVQSADFAPIA
                     AADLKVVTRRAPTEQELGDLLFAWRVAKYVKSNAIVYCKDGMTIGVGAGQMSRVDSAR
                     IAKIKAENAGLAIPGCVVASDAFFPFRDGLDVLAQAGATAVIQPGGSVRDAEVIAAAD
                     EQGLAMVFTGFRHFRH"
     gene            25441..>26303
                     /gene="purD"
                     /locus_tag="PLH85_09040"
     CDS             25441..>26303
                     /gene="purD"
                     /locus_tag="PLH85_09040"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNB64747.1"
                     /translation="MKILVIGSGGREHALAWKLAASQRVQKVFVAPGNPGTAREPGVE
                     NVALSAIPELVAFARSEQIALTVVGPEAPLAAGVVDAFRAAGLPIFGPTRLAAQLESS
                     KDFAKQFLLRHHIPTARYQTFSDPLAAHAYVDAEGAPIVIKADGLAAGKGVVVAMSVD
                     EAHAAIDAMLTHNTLGVQYDERGPRVVIEEFMDGEEASFIVLADGRHALPLATSQDHK
                     RLKDADQGPNTGGMGAYSPAPVVTPEIHARVMREIINPTLAGMAADGLPYTGFLYAGL
                     MIDAKGAPRVV"
BASE COUNT         4456 a        10211 c         7930 g         3706 t
ORIGIN      
        1 gccgccggcc gcggcgaacg catgcgcccc ctcaccgaca cctgccccaa gccgctcctg
       61 gcggcggggg gcaagccgct gatcgtgtgg cacatcgaac gcctcgccac cgccggcatc
      121 accgagctcg tcatcaacca cgcccatctg ggcgccatga tcgaagccgc cctcggtgac
      181 ggccgccgct tgggcgtcac gatccactat tcgcccgaag gcagcgcact ggaaaccgcc
      241 ggcggcatcg cccgagccct gcccctgctg ggtgacgcgc ccttcctggt gatcaacggc
      301 gacgtcttct gcgatgcgga tttcgcgccc ctggcccgtg ccgcagccgg gctgcgcgcc
      361 gacggtcccc tcgcccacct gctcctggtg cccaacccgc cccaccatcc ggacggcgac
      421 ttcggcctgc acgcggacgg ccacatccgc gacgacggca gcggcacgcg cctcaccttc
      481 gctggcgtcg gcgcgtacca cccggccctg ttcgccggcg tcgatcccga ccgccccgcc
      541 aaactcgccc cgctgctgcg caccgccatg tccgacggtc gcgtccgcgg cacccgcttc
      601 gacggccgct gggtggacgt aggcacaccg gcgcgcctgc gcgaacttga ccaggccttg
      661 cgcaccggga cgccggattg acgtaagctc gcaaccaacg gcccgcgccc cggtccatcc
      721 ttcatccccg tccttcccgg cccgtcccat gtccctcgac atcaccccct tccaggcccg
      781 tcgccaacgc ctgctggcca ccctgcaagc cagcggcggc ggcgtcgcca tcctgcccac
      841 cgcgcccgag cgcctgcgca accgggacgc ccactacggc taccgcttcg acagctattt
      901 ctactacctg actggcttcc gcgagcccga ggcggtactg gtgctggtgg cgggcgacac
      961 cccgcgcagc ctgctgttct gccgcaacaa ggatgtggag cgggagatct gggacggttt
     1021 ccgcttcggc cccgaggcgg cgcgggacag cttcggcttc gacgaggcct acgccatcgg
     1081 cgaactggac gaccagcttg cccgcctcct cgccgaccag cccgccatct tccactccgt
     1141 cggctacgag cccgactggg acagccgcat cgccgccgcc ctcaaccggg tccgcgccca
     1201 ggcccgcagc ggcgcccgcg cgccggagca gatccgcgac atccgcagcc tcatcgacga
     1261 gatgcgcctc gtcaaggacg atgccgaact ggcgctcatg cgccgcgccg ccgacatctc
     1321 gggcgccgcc caccgccgcg ccatgcgcac cgcccgaccc ggcacccggg aatacgaggt
     1381 cgaggccgaa ctcctccacg agttccgccg cggcggcagc caggccccgg cctacacctc
     1441 catcgtcgcc agcggcgcca acgcctgcgt gctgcactat gtcgagaacg accgcgtcat
     1501 gcagaacggc gagctgctcc tcatcgacgc cggctgcgag ctggacggct acgcctcgga
     1561 catcacccgc agctttcccg tgaatggccg cttcagcggc cctcagaagg ccgcctacga
     1621 gctggtcctc gccgcccagg ccgccgccat cgccgagatc cgtcccggcg ccagctggaa
     1681 cgccggccac gacgccgccg tgcgcgttct cgcccagggc ttcatcgacc tgggcctgct
     1741 cgccggcagc ctcgacggcg tccttgaaac cggcagctac cggcagttct acatgcaccg
     1801 caccggccac tggctgggcc tcgacgtgca tgacgccggc gactacaagc tcgccggcga
     1861 atggcgcccg ctggtggccg gcaacgtgct caccgtcgag cccggctgtt acatccgtcc
     1921 cgccgacggc gtgccggaag ccttctggaa catcggcatc cgcatcgagg acgacgccct
     1981 cgtgacgccc gacggctgcg agatcctcac caccgcggcc cccaagtccg tcgccgacat
     2041 cgaagccctg atggccgatc gctgacatgg accgccacga cctcgtcatc gtcggcgccg
     2101 gcccggtggg catggccctc gccctggccc tcaaggacgc cggcctcgac atcctcctgg
     2161 tggacgcgcg cgcccgcggc gcggcccggc acgatccccg cgtcctcgcc ctcgcccacg
     2221 gcacccgcct caccctcgaa cggctgggcg tctggacaca attgcgggcc acccccatcg
     2281 agaccatcca cgtctcccag cagggcggct tcgggcgcag cctgctgcgc gccaccgact
     2341 atgaccagcc cgccctcggc tacgtggtcg ccgccggtga tctggccgcg gccctcgacg
     2401 atgccctgct cgccgcgcgc atcccggtgc gcggcgacgc cagcgtgagc ggcctggccg
     2461 ccggcgccga cgacgtgctc gtcagcctcg ccgacggcca taccctgtcg gcccgcctcg
     2521 ccgcctgcgc cgaaggcgcc atccagggcg acgaggacga caccctggtg gagcgggact
     2581 acggccagca cgcggtgatc tcggtcgcca cgcccgccgg cagccacggc caccgggcct
     2641 gggaacgctt taccccgcag ggccccctcg ccgtcctgcc ctgcggtcac gactgcgcca
     2701 tcgtccacac cgcggcaccc gacgcggccg acgccctgat gaccctcgac gacgcggcct
     2761 atctggcgca cctgcaggcg cacttcggcc agcgtctgcg ttttgccgcc gtcggtccgc
     2821 gggcccgcta tccgctgcgc ctgcgctacc ggccgaaccc ggtgggccac cgcaccgtct
     2881 ggctcggcaa cgccgcgcag acgctccacc cggtcgccgg ccagggctac aacctggccc
     2941 tgcgtgatgt atgggcctgc gcccaggtgc tgctgcgcag cggcggcgat cccggcgccc
     3001 cggagaccct cgccacctac gcccgcgaac gccgcctcga ccgggccggc acgatccgct
     3061 tcaccgatac cctggtgcgc ctgttcagca cggatttggc gcccctgcgt cacgcccgcg
     3121 gcgccggcct gttcgccctg gatctgctgc cgccggcacg ccatttcgtg gccaagcgga
     3181 tgatgttcgg cgcccgcgcc tggccgtgag cccggcgggc tgaagtcccg cccggcgccc
     3241 tgcacgtccg cctccccgcg gcgcagcaaa cgccgtagaa tccgcccctc cgcgtcccca
     3301 tccgggggcc tcgtgccgag tggaccgcca tgtccgaaac cgacgacctg cagcaccacg
     3361 acgacctcga ccgccacgtg cgcgaggtgc agcatctgct cgcccggcac aagctggtgg
     3421 aggatctcgt ccatcgccag gacatgcccc gccacgatct ggtggagaac ctggtccaca
     3481 agcagcacgt caacgagctc actcacaagc tcgacagcct ccacccggcg gacatcgccc
     3541 acatcctcga agccctgccc ctcgatgaac gtctcttcgt ctgggatctg gtcaaggccg
     3601 agcgggacgg cgagatcctg ctggaagtct cggacgcggt gcgggaatcc ctcatcgagt
     3661 ccatggacac cgccgcgctg caggccgccg ccgagcagct cgacgcggac gaactggccg
     3721 acctggcgcc ggacctcccc cacgaggtca tggaggcggt gtatcagggc ctcgcccagg
     3781 aagaacgcga gcaactgcgc gccgccatgt cctatccgga ggactcggtc ggtgcgctga
     3841 tggacttcga aatggtcacg gtacgcgagg acgtgaccct ggaagtcgtg ctgcgctacc
     3901 tgcgccgctt cgaggcgctg ccggaccaca ccgaccagct cttcgtggtg gatcgggacg
     3961 aacgcctgcg cggcgtgctg cccctcaacc gcctgctcct caacgacccg gacgtgacgg
     4021 tgagcgacgt gatgctcacc gacaccctca ccctggagcc agccgacgaa gccgaaaccg
     4081 cagcccaggc cttcgagcgc tacgacctgg tgtcggcgcc cgtcgtggat taccaccgca
     4141 agctggtggg ccgcgtgacg gtggcggcgg tggtggactt catccgcgag gaaaccgaga
     4201 acgagctgct cagtcaagcc ggtctgaagg aaggcgaaga cattttcgcg ccggtgtggg
     4261 actcggtgaa gaaccgctgg tcctggctgg ccgtcaacct ggtcaccgcc ttcatcgcca
     4321 gccgcgtcat cggcgccttc gagaactcca tcgaaaagct ggtggccctc gccgcgctga
     4381 tgcccatcgt cgccggcatt ggcggcaact cgggcaacca gaccatcacc atgatcgtac
     4441 gcgccatcgc catgggtcag gtccagcacg acgccctgcc gcgcctgctc aagaaggaac
     4501 tgggtgtggc gctgctcaac ggcctggtgt ggggcgggct gctcggcgtg ctggcgtgga
     4561 ttctctacgg cagcggcaag ctcggcgcgg tgatgaccgc cgccatgacc ctcaacctgc
     4621 tggtcgccgc cttcgtgggc gtgatggccc cgatgatccg ccaccgcttc ggccaggacc
     4681 cggcggtggg ctccagcgtc atcatcaccg ccgtcacgga ttccggcggc ttcttcatct
     4741 tcctcgggct ggcgacgatc ttcctgatgt aagcaccggc ccggggccag cggcaacgcg
     4801 cgctggaaaa gcgggcccac ggcgactacg gtgacaggaa cgcccgtccc cgttgccgcc
     4861 cggaaaggtt cacgccatga agctcgctgc caccctcgcc accctggcgc tgtccttcaa
     4921 cgccggggct gcggaattca acgcgccctg gaacgatgcc cagatcgccc tcgtcatcga
     4981 tccgtacgcc gccaacagcc tgaagtggga cgccctggcc ggcgaaccac gggtcgtcgc
     5041 gatcatccac aaggcgacca tcggcaccca gggcctcgac aaggcctact ggacccgcaa
     5101 ggccgaagcg cgccagcggg gttacctgtg gggctcctac cactggggcg tggccggcga
     5161 tccggaaaag caggcagact actatctgga tacggtcaag ccgggcgacg atgaactgat
     5221 cgccctcgac ctggaagacg ccacgtccaa gaccctcatg tcggcggccg aggcggtccg
     5281 cttcatcgag cgcatccacg cccgcagcgg gcgctacccg gtgctctaca ccaaccacgc
     5341 cagcgcggtg ctgatctcga agacctacaa gaacagcgtc tttgcccaca cgcccctgtg
     5401 gtacgcgcgc ttcaagaccc aggttacgga tttcccgaag ggtctgtggc ccacctacac
     5461 gctgtggcag ttctccagcg agatcaagcc ccagaaggcg atccccggca cccgcagcga
     5521 catggacgtg aatgtcttca acggcagcgt cgaacagctc cgcaccgcct ggccgctgac
     5581 acagcgcgcg ccctgaggcc gggccgcgcg ccgggcacgg tgctcagccg ccgttcgccg
     5641 caaaccaggc ggccgccgtg gcaatggtgg aatcgatgtc cgcatcggtg tgcgctgccg
     5701 acacgaagcc cgcctcgaag gccgacggcg caaagtagtg accggcgtcc agcatggcgt
     5761 ggaagaagcg gttgaaggtc tcccggtcgg aggccatcac gtccgcgtag gacgtcggca
     5821 ccgccggcgc gaagtacagc ccgaacatgc cccccaccgc gtcggcagtg aagcgcacgc
     5881 cgtgctgcgc cgccgccgcg gcgagaccct gaacgaggcg cagggtgcgc gcggtgaggc
     5941 cgtcgtagaa gcccggccgg gcgatctggt tgaggctggc gagccctgcc gccaccgcca
     6001 ccggactgcc ggacagcgtc cccgcctggt acacggcgcc cagcggggcg atcttctcca
     6061 tgatgtcgcg acggccgcca aaggcgccca ccggcatgcc gccgccgatc accttgccca
     6121 gggtggtgag atccggcgtg atgccgaaga ggccctgcac gccccgcggc cccacacgga
     6181 agccggtcat cacctcgtcg aagatcagca ccgcgccgtg ctcggtgcac aggcggcgga
     6241 gcaggtgcat gaactcggcc gagggtttga ccagattcat gttgccggcg atgggctcga
     6301 tgatcacgca ggcgatctcg ttgccccgcg ccttgaaagt ctcttcgagc tgggccgggt
     6361 cgttgtagtc gagcacgacg gtgtgcttgg cgaaatcctc gggcacgccg gcggaagacg
     6421 ggttgccgaa ggtcagcagg cctgagccgg ccttcaccag caggctgtcg gcgtggccgt
     6481 ggtagcagcc ctcgaacttg acgatggcgt cgcgcccggt aaaaccgcgg gccaggcgga
     6541 tggcgctcat ggtggcttcg gtgccgctgg aaaccagccg caccatctcc aggctgggca
     6601 gcagctcgca caggcgctcg gccatctcga tctcacgctc ggtgggcgcg ccgaaggaca
     6661 gcccgtccag cgccgcggca cgcaccgcct cgaccactac cggatccgcg tggccgagga
     6721 tggcgggccc ccaggacccg acgtagtcga gatattcctt gccgtcggca tcccacaccc
     6781 gcgccccctg ggcgcgctgc aggaagcggg gcgtgccgcc caccgagcgg aaggcccgga
     6841 cgggcgaatt gacgccgccg ggaatggtgc gctgggcgcg ggcaaagagt gcttcgttgc
     6901 ggctggtcat acggacgatc ctggaattca gtcgaaaagc cggccatagg ccgccgcgcg
     6961 ggcggccggg tcgggcgcgg aaaacacgtc gctgatgacg gcgagaagat cggcccctgc
     7021 cgcccgcagc aagggcgcat tatcgacggt aatgccgccg atggcagcca ccggccgtcc
     7081 aaaagccgcc ttggcgcggc caagcagcgc cagcggcgcc ggcggcgcag agggcttggt
     7141 ggacgacggg aacatggcgc cgaaggccag atagtcggcg cccgcggcgg cggcggcctc
     7201 cgcccggggc cattctgcgt aacagctcac gccgaggaga cgatggggcc cgagggcagc
     7261 ccgggcggcg gcgaggtcgc cgtcgtcgcg ccccagatgg acgccgtccg cgccgcactc
     7321 caatgccagc gccagatcgt cgttgatcac gaggggcacg ccggcggcgc gacacagggg
     7381 cagcaggcca gccacctgga cgcgccgcag ggccgcatcg gcaagcttgt tgcggtattg
     7441 cagcaaggcc ggtcgggtgg cgagcaaggc agggagcatc gccacgagcc gcccggtgtc
     7501 ggtccagtcg ggagtgacca ggtagaggcc gcgccgcagc acgggatcag tcttcgccat
     7561 cgtccaccgg gcgggcccag aagaagcggt ccgggatgtg ctgccccatg ccggggcgga
     7621 aggcgtaggc cagggtctgc caggtaaatt cctgagcgtc gcgcaccgcc acgggcacct
     7681 ccagccccct tgccagggct gcggccaccg ccgacgcgag ggtgcagccc gagccgtgat
     7741 aggcgccggg aagccgctcc cacgtatcgg cgcgcaccac ccctccgcgc ccgtacaggg
     7801 tgttgatcac ctgcacgctg tgctcatggg aacccgtgat caagacgtat tcacagccca
     7861 tggccaccat tcggcgggca cattcggcca aggtgggggt gtcgtcgtcg tcgcgatgac
     7921 gggccgccag gcggcgggcc tccagcgtat tgggagtgac cacggtggcc tggggcagga
     7981 tcagctcgca aagcccttcg agcatggact cggaggccag ctcgtctccc cgcccggagg
     8041 cgatcaccgg gtccaccacc accgggacgt ggggatagtc ggcgaggatg cgggcgatga
     8101 cctcaatgtt gtcgaggctg cccagcacac cgagcttgaa ggcctggacg ggcatgtcct
     8161 cgagcagcgc ccgggcctgc tcgtcgacga attcggcatc ggtggggagg accgcctcga
     8221 caccggcagt gtcctggacc gtcagggcgg tgatgaccgt caacgcatga caccccatcc
     8281 cggagagggt catgatgtcg gcctggatac ccgcgccgcc ggtgggatcg gaggcggcaa
     8341 acaccatgac gagtggagga gagggatcgc tacgggtgga aatcatcgga gcatgcgagg
     8401 acgcacaaat tttacaacga ctgcgctacc attcacgatc caagcgattc tagcccaatt
     8461 caccccgctc ccacatgctc acccccgaat acaaaacctg gatgtgtctg atctgtggct
     8521 acatctacga cgaggcggcc ggtgcgccca acgagggtat tgcccccgga acgcgctggg
     8581 aggatattcc tcccaactgg acctgccccg aatgcggggc gcgcaaggac gactttgaaa
     8641 tggtcgccat ctgaaccttc aaaacatgac atcatgatat acccaaatgt catgcaatcc
     8701 gagcccggag aatcaaggtg aacctgaacg gcatcaaggt catggtcatc gacgacagca
     8761 acaccatccg tcgcagcgcc gaaatcttcc tggcgcaggc cggctgtcac gtggtgctgg
     8821 ccgaagatgg ctttgacgcc ctggcgaaga tcgccgacca cgccccggac ctgatcttcg
     8881 ttgacatcat gatgccccgc ctggatggct accagacctg ctccctgatc aagaagaacg
     8941 cccgactcaa gtccacgccg gtgatcatgc tgtcctccaa ggacggactg ttcgatcggg
     9001 cgcgaggccg tctggccggg tcggatgagt acctcaccaa gcccttcacc aaggaaagcc
     9061 tgctgcgttc cgtggcgagc catgtcccgc cccgtgcggg cgcagccaac tgatcctcac
     9121 gccatcgtcc gcccatgccg atcaagaaaa tcctcgtcgt agacgactcg cccaccgaaa
     9181 gactggccct gtccgaggtg ctcgtgcgcc acggctacca ggtcgtgacc gccgacagcg
     9241 gcgacgaggc agtgatcaag agcaagaccg aacagcccga cctgatcctg atggacgtgg
     9301 taatgcccgg caccaatggc taccaggcca cgcgcaccat cgtccgcgat gagcagaccc
     9361 gcaacatccc ggtcatcatc tgcaccagca agggccttga aaccgacaag atctggggca
     9421 tgcgtcaggg cgccttcgac tacctggtca aacccctcga ccacgccatg ctgctcgaca
     9481 agatcaaggc cctcggttga cgggatcatg gccaagcgca tcagccttca ggaatttcag
     9541 gccggcctcg cccggcgtct cgccgaagcc cgcgacaccc gcgcctcggc ccttctcgcc
     9601 gttcaggccg gttcgcgcaa ctggctgctc gatctggccg aggctggcga gatcctcgcc
     9661 gtgccacctc tggcgccggt cccgctggtt catccctggt tccgcgggct cgccagcgta
     9721 cggggcaacc ttttcggcgt cgtggacttt gccgccttcc acggcgaagc cccggtggtg
     9781 accggcggac aggcgcgcct gctgctcgtc ggcgcgcgcc tcaacctcaa ctgcgccctg
     9841 ctcgtctcgc gcgccctcgg cctgcgctcg gtggacgatt tcgagcccct gtccgagccc
     9901 gaggacgcca ccaccccgtg gatcggcgcc cagttgcagg atgcccacgg ccacacctgg
     9961 acacggctgc gggtccaggg tctgctgaat cattcacgct ttcttgacgc tgcactgaca
    10021 tgaattcgaa tcggggagga gcagcgggca tcacccgctg aaaagcaact atgggattca
    10081 agctacctgg gctgtccttt ggcagcaaaa tggcgaccaa caccggcgac aacaccatcc
    10141 tcgaagccag cgagcgcggc gaggtgagcg cggtgctgcc cttcctggcc ggcaagagta
    10201 cggccgagca gttgcgcctg ctgcgcctgc ccttcattgc cttcgcggtc ctgactgcgg
    10261 tgctggtgat ctaccagatc cgcgtcagca gctttggtac cgcctacgtc acggcagcag
    10321 gccagatgcg cacgctgtcc cagcagatgg ccaaggccag ccagctcgcc ctgcagggtg
    10381 aagccggcgc ctttgccgaa ctcaagcgca gtcgagaaca gttcaaccac ctgctcgaag
    10441 cggtcaccca gggcggcgac gtggacggca cgggcgtgcc gggcggcagt tcggcggtcg
    10501 ccgacgagct gaccgcggtc accgagctgt ggcagaagtc cgacaaggac gccggccagc
    10561 tcctcggcca ggaaaagaac ctggcgagcc tgggcaatgc cgttgccacc atcaacacca
    10621 aaaacgccca cctcctcgaa ctcaccgaac aggtcgccgc cctcaagctg cagaccggcg
    10681 cctccgcgcg ggaaatcgcc accgccaacc aggtcgtgat gctgacccag cggatcgcca
    10741 agaacgccaa cgccctgctc gtcgccaacg cgatcgaccc ggaagtggcc ttcctccttg
    10801 gcaaggacac caacaccttc cgcgagcaga tcgaagccct gatgaagatg actgccgccg
    10861 gctcagacgc ccagtccaag ctggtggagc tcgaaggcgt ggccaaggat aacctggccg
    10921 ccgtgcaggc catcctcggc aacatccagt tgctcgtcca ggccaagcag tccggcagca
    10981 acatcttcca gaacagcggc ccgttgctgg aaaagacctc ggccctcgcc gaaggctact
    11041 cccgcgcata tacgggcttc ttcacctggg tcaccgtcgg catcatgctg tttgccgtcc
    11101 tgtccatcgt cctgctgctg ctgatggcca aggtctataa cgatgacatt gcccgccgcc
    11161 gcttctctgc cgaacgtctg cgcgagagcg cagagtccga gcgcaacagc acccagcagg
    11221 ccatcctgcg cctcatgaac gagatgggcg acctcgccga cggcgacctc accgtgcgtg
    11281 ccaccgtcac cgaagacatc accggcgcca ttgccgactc ggtgaactac accattgaag
    11341 aactctccgt gctggtgcgc cgcatcaacg acgccgccag ccgcgtgacc agcgccaccg
    11401 aatccgccca gcgcacctcc gacgaactgc tcgccgcctc cgaacggcag gcccaggaac
    11461 tccgcctcgc cggcgatacc gcccacagca tggcggtctc catgagtgaa gcctcggaag
    11521 gcgccctgca gtccgcccag gtggcccgcc gcgccctgga agccgcccag aaaggggcca
    11581 ccgcggtgga ggacaccatc aagggcatga acgacatccg cgacaagatc caggacacct
    11641 ccaagcgcat caagcgcctc ggcgaatcgt cccaggagat cggtgaaatc gtggaactga
    11701 tctcggacat taccgaacag accaacgtgc tggccctcaa cgccgccatc caggccgcct
    11761 ccgccggtga agcgggccgc ggcttctcgg tggtggcgga agaagtgcag cgcctcgccg
    11821 aacgttccgc cgaagcgacc aagcagatcg gcgccatcgt caaaaccatc cagaccgaca
    11881 ccgccgacgc cgtggccgcc atggaaaacg ccacccgcga cgtggtggat ggcgccaccc
    11941 tgtccgaaac cgccggtcgc gaacttgccg agattgagcg gatctccgcc gaaaccgccg
    12001 aactgatcga acgcatctcg gtcagcaccg aaatccaggc caaatccgct acccaggtgg
    12061 ccgaaaagat gaagggcatt cttgccgtaa ccgaccgcac cacggcgggc acccagcaga
    12121 ccgccgtctc catcggcgaa ctggccgacc tcgccgtgga gctgaagggt tcggtctcgg
    12181 gcttcaaggt ctgaccccgc accgtcacct ggaccggaca ccgaccggaa tgcatcgatg
    12241 agctccgcac acgaactcga ccttggcccg ctgacctggg tcaaaggcga aatcgacctg
    12301 gccctggaac gcgccgccgg cgccctcgcc gaagccgaga cggcctccga ccgccttggc
    12361 cgcatcaagt tcgcccagac ccacctgcac caggcccacg gtgccctgtc catcgtcggt
    12421 ctggatggcc tcacccagtt ttcggaagcc ctcgatcgtc tgctgggcga gctggccgcc
    12481 gaacaggtca cctacacgcc ggccatcgcc cgcctcgcca cccggggact cgccgccctg
    12541 ggcaattacc tcaacgaact gacccgcggc cagcccgacc agccgctgcg tctctttgaa
    12601 ctctacgcgc agctcgtcga agcccgcggg catgaggcac cgggcccgtc ggacctgttc
    12661 taccccgacc tctccctgcg tccggccttc gcaccgctcc ccgagggtgg cacggacgat
    12721 gcgcgcaccc tgcgcgccct gcgcggccga tttgagcgtg gcttgctcaa atggctgcgc
    12781 aatcctggcg acgccagcgg tcctgccgag atgcgcgatg ccgtcaacgg gatcgaacgt
    12841 actcagcgcc aacccgcctc gcgcgcattc tggcttgcca gcgccgcctt cttcgatgcc
    12901 ctcggccagc aggccatcga cgtcgatccg cccctcaagc gcctctgcgg gcgcatcgat
    12961 gcccagatgc gacgcctcct cgaaggctct gccgtcgtcg cagaacggct gatgcgggac
    13021 gtgctctatc aggtcgccat cgcccgctgc aacacgcccc ccgtcaacac cctgcgcgaa
    13081 gcctatcggc tcgtcaacct gatcccggcc cccggccgga gcgtgagcga cgcaccggtg
    13141 gcgcccctgc tggtggcgct gcacagcggg ctcggtgatg cgcgtgaagc ctggaccctg
    13201 ttcaccggcg gcggcgccat cggcctgccc cgtttccagg acgccctcgc gcccctcccc
    13261 gccaccgccc gcgcgattgg ccaaccccat accgccgcgc tcatcgaagg gctcggccgc
    13321 ctggcgcagt ggctgcgcca ggatcccctg cgcctcaccg acaccctgtc catggacatg
    13381 gcgacggcca tgctgcttgt tgagggcctg gtggacagtc acgaaatgcc gtccccgggt
    13441 ccgcgcctca agaagcagat tgccgcagtc accgagcgca tccaggccta tctgcggggc
    13501 gaacagccag ccccgctgtc catcgccgac cccgacacgg ccgatcagca actgggtcgc
    13561 cagcttgccc atgaaatcct cgccaacctg ggcgacgtag agcagcatct cgacggattc
    13621 ttccgggagc ccgcccgcac cgacctgctg gccgccacct cggcgcccct caaacaggtc
    13681 gaaggcgcac tggcgatcat gaaccagggc gccgctgtac gcctcgtgca ggactgtgcg
    13741 gccgccatct ccgcgctcga gagcggtcgc cagtcgccag aagcggccat cttcgaagac
    13801 atcgcccacc gcctctcggc cctcggcgtg ttcatcgacg ccctccagca cggaccggcg
    13861 gatctcgaac agatcctcaa cccaagtacc ggtgacagcg ccggcgacag cctcgaagcc
    13921 gaactcgtcc agcaaaagcg cgaaaccgcc gccctcgccg ccgccctcca ggaaaacccg
    13981 gacgacatcg gcctgcgcac cgagctgctc ctcaacctcg aaggcctgcg ccaggatgcc
    14041 agcctcaccg ccgacgccga actcgaaaag caggcccgcg aggccctcaa cctgctcgat
    14101 gccggcgccc ccttcgctgc gccaccggcc atcgtgcccg aagcaatggc gcccgcactg
    14161 tcgcccgagg cggcgcgtct gctggatgcc agcgacgcag agatcgacgc cgaactgctg
    14221 gacatcttca tcgaggaagc cagcaccgtt cgtgacacca tcgccagcca tctcgacctg
    14281 ctgatcgccg cccccggcga tcaggaacac ctcaccacca tccggcgcag cttccatacc
    14341 ctcaagggca gcgggcgcat ggtcggcctc aaggatttcg gcgaggccgc gtggggcatg
    14401 gaacagaccc tcaaccactg gctgcgcctc gaacgcccgg ccacaccgga actcctggcc
    14461 ctcatccgcc gggcccacgt cgcggtgagc gactgggtgg atcagttgca ggcgggcggc
    14521 ggactgtccc tggacgtggc ggacatcctg cgtgaggcag agcggctgcg gggcgaagaa
    14581 gaccacggcg cggcgctcga gatcgtccag aacctggaat cgtcgatcct ccagcccatc
    14641 atcgaactcg acgcgccgcc ccctgcgccg gccgtcgaat ttcccgcgct cgattttgac
    14701 tcgctcgaag catccggcga gcgcccctcg accgaccgcc tggacatcgt ccttgacgac
    14761 gcccctgccg agcctcccgt cgcactggaa gagctcgtct gggcagaacc acccgcagtg
    14821 cccgcgcccc cggcccccac cgccgccgaa cctgcgccat cggagctcgc caccgacgcc
    14881 atcgaaatcg agattgaaat cgaagcggcg gcactggaag acgacgacgt gttcccggaa
    14941 gagggtgccg aggtcatcga tctgggcgca cctgaacccc ttgagcccga cgcgagcgaa
    15001 gcggtcgacc tgcccgaatc cgacgccacc gcagaggcca tcgaactcgg cgcggccgcg
    15061 ccggagtccg tcgcagacgc cccgtcgcac gggcgggaac acgacaccga ggatatcgac
    15121 agcctcgagg acgacgatgc cgtgcgcctc ggccatgtcg tcctgtcgcg ccccctgttc
    15181 gacctctacc tcgccgaagc ccgccagcac ctggccaccc tgcgtcacga actcggccgt
    15241 atggccgtca atcccagcct gctgccggcc aacgcggcga tccgcgccgc ccacaccctg
    15301 tcgggcatct ccggcacggc ccaattcacc gcaccgcaaa gcctggccaa ggccctcgaa
    15361 ctcgcccagc aacgcctcgc cgatcaccac caggcgccca gcgccgatca ggcccagctg
    15421 ctcaacgggg cggccaccgc cctcgagacc atgctcgccc agctttccgg gcgccgactc
    15481 cccgacgccc agcccgagct cgtcgacgct ctggacaacg tgctgcgcgg ccacctggcc
    15541 gaagtcatca ccctgcggcc ggaaataccc tccgacctgc cacccccgga gctcgccacc
    15601 gcaccgctgg cgcgcagccc ggaacccgtc acgccgccgc ccgaggcgcc ctccgcggcc
    15661 gctccggtcc tgcgtgacga gctcgacgcc gaactgctgc ccatcttcct cgacgagggt
    15721 aacgaactcc tccacagcct cgacaccgcc ctgcgcgcct ggcgcgccca gccggacgat
    15781 gcgacccagc cggccgccgt cgcccggctg ctgcacaccc tcaagggcag cgcacggatg
    15841 gtcggcgcca tgagcttcgg cgccagcatc cacgagctgg aaacgcggct cgagcgcgcc
    15901 cgtcgcctcg ccccggacga catcgcgatc gtcgacgaca tcgagtccgc gctcgaccgc
    15961 agccggcaga ccctcgaaac cctcgggatc agcccgcccg ccacaacgcc ggccgccgtg
    16021 gccgcggccc ccatcgatgt cgagacaggc gcagtcgagg cagtgccggc cggcgccgac
    16081 ggcacgctcg cggccccccg ggcccaactg cgggtcgaag ccaccagggt ggaccagttc
    16141 gtcaacgagg ccggcgaaat cagcatcgcc cgcacccgca tcgaaggcga actgcgcacc
    16201 ctgcgccgct ctctgctgga cctcaccgaa aacgtcatcc gcctgcgcaa ccagctgcgc
    16261 gaagtggaaa tccaggccga cacccagatg cagtcgcgcc tgtcccaggt cgaaacccgc
    16321 cataccgaat tcgaccccct ggagatggac cgctacaccc gcctccagga actcacccgc
    16381 atgatggcgg agagcgtcgg cgacgtgacc accgtgcagc agaatctgct gcgcaacctg
    16441 gacggcgccg aaagcgccct gcatgcccag tcgcgcatga cgcgggacct gcaacagtcc
    16501 ctgatgcacg tgcgcatggt gcccttcgac agcctcgccg agcggctcta ccgcctcgtc
    16561 cgacagaccg ccaaggacgt cggccggcgt gccaacctgg acatcatcgg tggccagatc
    16621 gaaatcgacc ggggggtgct ggaggccatg accggccctc tggaccacct cgtgcgcaac
    16681 gccgtggcgc acggcatcga actacccgac gcccggcgcg ccgccggcaa ggccgagatc
    16741 ggccagatca ccctgcgcat cacccacgaa ggcaacgaag tggtgatcga attccgcgac
    16801 gacggcgccg gtctggattt cgatgcgatc cgcgcccacg cgatcgccac cggcctgctc
    16861 ggcaccgacg aagagtccga cgacaagcgc ctggccaatc tcatcttcct gcccggcttc
    16921 agcaccgcca ccctgtccag cgtcgccggc cgtggcgtgg gcatggatgt ggtcaagagc
    16981 gaaaccgccg ccgtcggcgg ccgcctcagt gtggaaaccg accgcggtcg tggtaccacc
    17041 ttccgcatcc acctgccgct cacgctggcc gtcacccagg ctctgctggt gcgttccggc
    17101 ggccggacct gggcgatccc ctcgtccatg gtcgcccagg tgctcgaact caaggccgcc
    17161 gccctcgaaa aggtccgcgc cgagcacggc accgactggc tcggcgagcg ctttgcctat
    17221 cgatatctgc cgcgcctgct cggcgacagc gccacccagc cccaggttga acgcttcaac
    17281 tgggtgctcc tgctgcgcgc cggcagcgaa accctggcgc tgcatgtgga cgccctccgc
    17341 ggcaaccagg aagtcgtcgt caagaacgcc ggcccgcagt acgcccggat ggtcggcttc
    17401 tccggcgcca ccgtgctcgg cgacggcgag atcgtgctga tcctcaaccc ggtggccctc
    17461 gccgcccgtg cgcccgacgc caccgacgac ggcgtaccgg gtctgcccgc cgctccggcg
    17521 attcccgagg tgccgctggt catggtggtc gacgactccc tcaccgtgcg caagatcacc
    17581 ggccgcctgc tggaacggga aggttaccgg gtgctcaccg ccaaggacgg cgtcgacgcg
    17641 ctggagcaac tcggccacaa cgacccggac gtcatcattg ccgacatcga aatgccgcgc
    17701 atggatggct tcgacctcac ccgcaacatc cgcgccgatg cccgccacaa gcgggtgccc
    17761 atcatcatga tcacctcccg catggccgag aagcaccagc gttatgcaga ggagatcggc
    17821 gtgaaccact acctgggcaa gccctacgac gaagatctgc tgctcaggct gatccgccgc
    17881 tacacccgcg cctgacggac cgtgccgtgc cctcgccccc gaggggcggc cgggcttggc
    17941 tataatcggt cgttttcctg tccggccgtc ccatgaatct gctcttcgaa gaggacggtg
    18001 ccttcaagac cggcaccgtc ctgaccgaca acgattccac cctccaggtg gaaactcccc
    18061 atggcaagcg cctcaagctc aagagcagcc acgtcctcct gcgcttcccc cagcccgccg
    18121 ccggcgaact gctggatcgc gccgagatcg aggccgaagg cctcgatacc gaattcctgt
    18181 gggacgtctg cggtgacgac gaattcggtt tcgccgactt cgccagggaa tactacggcc
    18241 acgcccccac ccccatcgaa gcgaccgccg tgctgctgcg cctgcacgcc gcgcccatct
    18301 acttccaccg caaggggcgc ggccgcttcc gcaaagcccc ggccgacatc ctccaggccg
    18361 ccctcgccgg cctcgagaag aagcgccagc aggccgccgc catcgagcgc atgcgcgacg
    18421 cactgctcgc cggcaccctg cccgaagagt tccgcccgct gatccgcccg cttctcttca
    18481 agcccgaccg caaccgcctg gagaccaagg cccttgaagc ggcctgcgcc gagtgcggca
    18541 aatccgccgc ccgcctgctg ttggactgcg gcgccctggc ctcgtcccaa gaactgcact
    18601 tcgaccgttt cctgtacgaa tacttccccg aaggcaccgc cttccccagc gtcgacgcgc
    18661 ccaccgcgcc ggccgacctg ccccttgccc cggtacgcgc cttctccatc gacgacgccg
    18721 ccaccaccga gatcgacgac gccttctccg tcaccgccac gcccgatggc tggcgcatcg
    18781 gcatccacat cgccgccccc ggcctgggct tcggccgcga ctccgccctc gacgccatcg
    18841 cccgcacacg cctgtccacg gtctacatgc ccggtcacaa gatcaccatg ctgccggacg
    18901 tcgtcgtgga aggcttcacc ctgggtgaag gccgcgactg cccggccctg tccctgtacc
    18961 tggagctcgc ccgcgacctg cgcatcaccg gccaccactc ggccctcgaa cgggtgcccg
    19021 tcgccgccaa cctgcgccac cacgacctgg agccggtgtt caacgaaacc accctcgccg
    19081 atggcggccc ggacttcccg tggaaggccg agctcaccct gctgtgggag ctcgccaccg
    19141 tcctcgaagc cggccgtggc aagcccgccg ccaaccagac cctggtggac tacgccttca
    19201 acgtcgattg gtccgtcgcc acaccggatg gccccggccg cgtggacatc ggccgccgac
    19261 cgcgcggctc acccctcgac aagctggtgg ccgaactgat gatctgtgcc aacagcacct
    19321 ggggcaaggc cctggctgac gccggcgttc ccgccctgta ccgcgcccag accggcggca
    19381 aggtgcgcat gaccacctcc gcagcgcccc acgaaggcct cggcgtcgac tgttacgcct
    19441 ggtccagttc acccctgcgg cgctacgtgg acctggtcaa tcaatggcag ctcatcgcct
    19501 ggctgcgcgg tgaggccccc gccttcccgc ccaagtcggc cgacctgatc gccgccatgc
    19561 gcgacttcga actcacctac gccgcctacg ccgacttcca gcgcggcatg gagcgctact
    19621 ggtgcgtgcg ctggctgcgc cagatcgagc gccctgagct caccgcccgt gtgttacggg
    19681 aaagcctggt gcgtctggaa gacattcccc tcaccttcaa gctgccgtcc atgcccaccc
    19741 tgccttccgg cacccgcgtg cggctggcca tcgacagcac cgacctgatc gacgtggagg
    19801 tgcgcgccac ctacctggaa accctcgccg acgccccgct cgagatggcc gaggcgctcg
    19861 ccgaagacgt cacggacgcg ccgctgcccg aagcggcccc gccggcttcg gacgcaccgc
    19921 ccgcgtcctg actcccgatg cagcaccctg ccccgctccc tcccgtagca ccgccgcccg
    19981 ccccgccctc agggatggcg gccgggcgtc tgccgctggt ggccccctcg cgcaaggcgt
    20041 cccgctatgc gctggagtgg ggcgtgggcc tgtccctgct gttgcacgcc ctcctgctgt
    20101 cggttcattt cgtgatgccc gacgccaacc acttcacgcg cacccatcag gatctcgccg
    20161 tggtgctggt caacgcccgc cacgcccagc gccccgacaa ggccgaactc ctggcccaga
    20221 ccaccctcga cgccggcggc accagcaccc ctgacgcccg cgcctcgacg cccctgccgc
    20281 cccagaccct gagccgcgcc ggcgatggcc tcgtcgaagc ccagcgccgc gccgaaacgc
    20341 ctcagacgcc ccagatgcag gccatgacgg cgccgtccgg caagaccgcc cttgccgtgg
    20401 acagccgcca gaccgcgccc accgagcccc cccgccaggt ctccggctac gacctcctcg
    20461 acagctccgc cgccgttgcc cgcatcgagg cccagatcga caaggacctg gtggactacg
    20521 ccgcccgccc gcgcaagaag ttcatcggcg cccgcaccca ggaataccgc ttcgcccagt
    20581 acgtggaaga ctggcgccag aagatcgagc gcgtcggcac cctcaactac cccgaagccg
    20641 cccgcggcaa ggtgtatggc agcctgctcc tgtccgtcac catccgctcg gacggcaccg
    20701 tggtcagcat cgacgtccat cgctcgtcgg ggcacaaggt gctcgacgac gccgcccggc
    20761 gcatcgtcca gctggccgcg ccctacgcaa tctttccgcc ggacatccgc cgcgacaccg
    20821 acgtgatcga gatcacccgc acctggacct tcaccaactc cgacatgctc cagacctccg
    20881 acaagaagga atgaggccca tggaccgcta cgccgtcatc ggcaacccca tcgcccacag
    20941 caagtccccg cgcatccacg ccctgttcgc cgcccagacc gctcaggccc tgagctatga
    21001 ggccctgctc ggccccctgg gcgccttcgc cgccaccgtg gacgccttcc gcgccgccgg
    21061 cgggcgcggc ctcaacgtca ccgtgccctt caagctcgac gccttcgcct acgccacccg
    21121 gcgcaccccg cgggccgagg cggccggcgc ggtgaacacc ctggccttcg ggccggacga
    21181 ggtgctcggc gacaacacag acggcgccgg tctgctgcgc gacatcaccg tcaacctggg
    21241 ctgtcccctg acggagcggc gcgtgctcct gctcggcgcg ggcggcgccg cccgcggcag
    21301 cctgctgccc ctgctgctga ccgaaccggc ccgcctcgtc gtcgccaacc gcagcagcgc
    21361 caaggccgtc gcactggccg ccgaattcgc ccgcctgccg tcctgtgccg tggccgtcaa
    21421 gctcgaaggc tgcggctttg ccgacctggc cggcgcccag ttcgacgtcg tcatcaacgc
    21481 caccagcgcc agcctcggcg acgccgcgcc tgatctgccc gcgggcctct acgcccccgc
    21541 cagcctcgcc tacgacatga tgtacggcaa gggcgacacc cccttcctgg ccgccgcccg
    21601 cgcccagggc gccgggcgcc tcgccgacgg cctgggcatg ctggtggagc aggccgccga
    21661 gagcttcgcc ctgtggcgtg gcgtgcgccc cgacaccgca ccggtgctgg ccgccctgcg
    21721 cgccggctga gcccgccacc cggcaccgcc ccatgaagac cttcgggcgc tggctcaaac
    21781 gcggactact gggcctggtg ctggttttcc tggcctggca cctgtggctg ctcggccacg
    21841 tcctgtggtg gaagtggcag cctccgtccg agaccagctt catgagcctg cgcctggacg
    21901 aacttgccac ccgccggccc gacgccgaac tgcgctaccg ctgggtgccc tacgagcgca
    21961 tctccgtcta cctcaagcgt gccgtggtgg ccgccgagga cgacaagttc accgatcacg
    22021 aaggcttcga ctgggacggc atccagaagg ccatcgagaa gaaccagaag aagggccgtg
    22081 ccgtggccgg cggctccacc atcagccagc agctcgccaa gaacctcttc ctgtcccccg
    22141 cccgcagcta cctgcgcaag gccgaggagg ccctcatcac ggtgatgatc gaaaccttct
    22201 gggacaagcg ccgcatcctg gaggtctacc tcaacgtggt ggaatggggt gacggcatct
    22261 tcggctgcga ggccgcggcc cagcgctatt tccacatctc cgccgcccag ctcggccccg
    22321 cccaggccgc ccgcctggcg gtgatgctgc ccaacccgcg caagtacgag aaggacttcg
    22381 gtccgcgcct ggctgcccat gcggcgcgca tccaggcgcg catgcagtac tccgacgtgc
    22441 cgtgacaggc gacgcacgca ggcgcatcgc cccctgcccg gccggcgccc ccatggggta
    22501 gaatgcgatg ctttccccct gcagtgcctc cccgatgcaa ttcgccggtt tcaccttgcg
    22561 aaacaatctc ttcgtcgcgc ccatggcggg cgtgacggac cgtcctttcc gccagctttg
    22621 caaaaagatg ggggcgggtc tggccgtctc cgagatggtc acctccaact ccctgctcta
    22681 cggcagcgcc aagacccagc gccgcgccaa ccacgatggc gagaccgatc cgatctccgt
    22741 gcagatcgcc ggcgccgatc cggccatgat ggccgaggcc gcacgccaca acatcgaccg
    22801 cggcgcccag atcatcgaca tcaacatggg ctgcccggcc aagaaggtct gcaacgtcat
    22861 ggccggctcc gcgctgatgc aggacgaagc cctcgtggcg cgcatcctcg aagccgtcgt
    22921 cggcgcggcc ggcgccacgc cggtcaccct caagttccgc accggctgga gcaaggccca
    22981 caagaacgcc gccaccatcg cccgcatcgc cgaaacctcc ggcgtgcagc tcatcgccat
    23041 ccatggccgc acccgcgcgt gccagtacac cggcgatgcc gaatacgaca ccatcgccga
    23101 cgtgaagacc cgtgtgcgca tcccggtcat cgccaacggc gacatcacaa cgccccacaa
    23161 ggccaggttc gtcctcgact acaccggcgc cgacggcgtc atgatcggcc gcgccgccca
    23221 gggccgtccc tggatcttcc gcgagatcga acacttcctg agcaccggcg aggaactccc
    23281 gccgccgctc gtcagcgaaa tccaccaggt ctgccgcgac cacctcgccg acctctacgc
    23341 cttctacggc gaagacaccg gcgtcaaggt cgcccgcaag cacatcagct ggtacaccaa
    23401 gggtctggcc ggctcggcgc agttccgccg ggtcatgaac accctgccga cagtacaaga
    23461 acaactcacc gccatggacg aattcttcga ccggctcgcc gacagagaca gccgcctggt
    23521 ctacgtggac ccggaagact ttcccaagga gcttgccgca tgagtcgcag cagcaacgac
    23581 atcgccgaat ccgtccatcg cgccctggag acctatttcc gcgacctgga cggcgagaag
    23641 cccaacgccc tctacgacat ggtgatccgc aacgtcgagc gccccatgct cgaattcgtc
    23701 ctccagcagg ccggcggcaa ccagaccctg tgcgccgaga tgctcggcat caaccgcaac
    23761 accctgcgcc gcaagctctc cgaataccag ctcctgtaac cgattcgccc atccccgccg
    23821 cggcccggcc cgcggccttc cagagaagcc catgaaagtc acccaagccc tgctcagcgt
    23881 gtccgacaag cgcggcgtcg tcgaattcgc ccgcgaactc tccgccctcg gcatccagct
    23941 cctgtccacc ggcggcaccg ccaagtccct gcgcgacgcc ggcctgcccg tcaccgacgt
    24001 ctccgactac accggcttcc cggaaatgct cgatggccgc gtcaagaccc tgcaccccaa
    24061 ggtgcacggc ggcatcctcg cccggcgtga cctgcccgaa cacctggcca agcttgaagc
    24121 gcacagcatc ccgcgcatcg acctggtggt cgtgaacctc taccccttcc aggccaccgt
    24181 cgccaagccc gactgcaccc tcgaagacgc catcgagaac atcgacatcg gcggccccac
    24241 catggtccgc gccgccgcca agaaccacgg caacgaagcc ggcggtgtcg gcatcgtcac
    24301 cgacccggaa gactacgccg ccatcgtcgc cgagctgcag gccaacgcgg gcgccctgtc
    24361 ctacaagacc cgcttcgacc tggcgcgcaa ggccttcacc cacaccgccc gctacgacag
    24421 cgccatctcc aaccacctca ccgccctggc cgaagacggc agcaagcagg cctaccccga
    24481 gcgcttccag ctcgccttcg acaaggtgca ggacctgcgc tacggtgaga acccccacca
    24541 gagcgccgcc ttctacaaag agtccgacgc ccccgccggc gccatcgccg cctacaccca
    24601 ggtgcagggc aaggaactct cctacaacaa catcgccgac gccgacgccg cgtgggaatg
    24661 cgtcaaggcc ttcgacgccg ccacccccgc ctgcgtcatc gtcaagcacg ccaacccctg
    24721 tggcgtggcc gtcgccgcca gcccgctgga cgcctacaag aaggccttct ccaccgaccc
    24781 cacgtcagcc ttcggcggca tcatcgcctt caacaccgtc gtcgacaaag ccgccgccga
    24841 agccgtcagc gcccagttcc tcgaagtgct gatcgccccc gcctacaccg aagaagccct
    24901 cgccctgctc aaggccaagc agaacgtccg cgtgctcatc gtgcccctgg gcgccgtcag
    24961 gcagaccgac tacaagcgcg tcggcggcgg cctgctcgtg cagagcgccg acttcgcccc
    25021 catcgccgcg gccgacctca aggtcgtcac ccgccgtgcc cccaccgagc aggaactcgg
    25081 cgacctgctc ttcgcctggc gcgtggccaa gtacgtcaag tccaacgcca tcgtctactg
    25141 caaggacggc atgaccatcg gcgtcggcgc cggccagatg agccgcgtcg attccgcccg
    25201 catcgccaag atcaaggccg agaacgccgg cctcgccatc cctggctgcg tggtcgcctc
    25261 cgacgccttc ttccccttcc gtgacggcct cgacgtcctc gcccaggccg gcgccaccgc
    25321 ggtgatccag cccggcggct ccgtgcgcga cgccgaagtc atcgccgccg ccgacgaaca
    25381 gggtctggcc atggtattca ccggcttccg ccacttccgc cactgatccg ggaccggccc
    25441 atgaagatcc tcgtcatcgg ctccggcggc cgcgaacacg ccctcgcctg gaagctcgcc
    25501 gcctcgcagc gcgtccagaa agtcttcgtc gcccccggca atcccggcac cgcccgtgag
    25561 ccgggtgtcg agaacgtcgc cctcagcgcc attcccgagc tggttgcctt cgcccgcagc
    25621 gagcagatcg ccctcaccgt cgtcggcccc gaggcccccc tcgccgccgg cgtggtggac
    25681 gccttccgcg ccgccggcct gcccatcttc gggccgaccc gcctcgccgc ccagctcgag
    25741 agctccaagg acttcgccaa gcagttcctg ctccgccacc acatccccac cgcccgctac
    25801 cagaccttct ccgatcccct cgccgcccac gcctacgtgg acgccgaagg cgcgcccatc
    25861 gtcatcaagg ccgacggcct ggccgccggc aagggcgtcg tggtggccat gagcgtggac
    25921 gaagcccacg ccgccatcga cgccatgctc acccacaaca ccctgggcgt gcagtacgac
    25981 gagcgcggcc cgcgggtcgt catcgaagag ttcatggacg gcgaggaggc cagcttcatc
    26041 gtcctggccg acggccgcca cgccctgccc ctcgccacca gccaggacca caaacgcctc
    26101 aaggacgccg accagggccc caacaccggc ggcatgggcg cctactcccc cgccccggtg
    26161 gtcacccccg aaatccacgc ccgcgtcatg cgcgagatca tcaaccccac cctcgccggc
    26221 atggccgccg acgggctgcc ttacaccggc ttcctctacg ccggcctgat gatcgacgcc
    26281 aagggcgccc cccgcgtcgt cga
//
LOCUS       DAOQMM010000044        26031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_580_length_26031_cov_47.341046, whole genome shotgun sequence.
ACCESSION   DAOQMM010000044 DAOQMM010000000
VERSION     DAOQMM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 26031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26031
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_580_length_26031_cov_47.341046"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLH85_09045"
     CDS             complement(<1..256)
                     /locus_tag="PLH85_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNB64748.1"
                     /translation="MSPGQQHLERFIFWSRWFQAPLYLGLVAAQGVYVYLFMAELAHL
                     IGSVFESGGHISETETMLVVLGLIDVVMIANLLIMVIIGGY"
     gene            360..1727
                     /gene="purB"
                     /locus_tag="PLH85_09050"
     CDS             360..1727
                     /gene="purB"
                     /locus_tag="PLH85_09050"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNB64749.1"
                     /translation="MNLHPITALSPLDGRYESKVAALREHFSEFGLIRNRVKVEIEWL
                     KALAAAPELAEIAPFSAATVAELDGVIAHFSVTDGEAVKAIEATTNHDVKAMEYWLKQ
                     RLGHNPEVTRVTEFIHFGCTSEDINNVSHALMLRDGRDGVLLPALDRIIARLVELAHN
                     LADLPMLSRTHGQPASPTTLGKEMANVVHRLRRVRASIAGVELTAKFNGAVGNYNAHL
                     SAYPDFDWEGFNGRFIESLGLTFNPYTIQIEPHDAMAELYDATARANTIVLDLCRDIW
                     MYISLGYFKQKLKAGEIGSSTMPHKVNPIDFENAEGNLGLANGVLRHLADKLPVSRMQ
                     RDLTDSTVLRNMGVAFGYAVLAYDSCLRGLGKLEADPARLAADLDACWEVLAEPIQTV
                     MRRYGVPNPYEQLKELTRGKGITRDALQAFIATLAIPEAEKTRLLAMTPASYIGKAAE
                     LARRI"
     gene            1785..2102
                     /locus_tag="PLH85_09055"
     CDS             1785..2102
                     /locus_tag="PLH85_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HNB64750.1"
                     /translation="MAFADKLKTLPGVSHLAALHLLDGAGQVVGTIENKPGQAGSLAV
                     YNHLAQLHGAITPDAATQGIELYAEHSEDARLNPGKHPNIDRLIGLVERGETLRVKHV
                     FAA"
     gene            complement(2163..2531)
                     /locus_tag="PLH85_09060"
     CDS             complement(2163..2531)
                     /locus_tag="PLH85_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64751.1"
                     /translation="MPLRFPPPVVRTAFALALVVIFWLAMIPLPEALTVFSFQDKVEH
                     TGAFVLLTLMGVTGFPLRRTAVAAGLVAYGLLIEVCQHRFTTNRVGDPWDWLADSVGV
                     FIGLRLAGWFARRVVNIARA"
     gene            2941..3351
                     /locus_tag="PLH85_09065"
     CDS             2941..3351
                     /locus_tag="PLH85_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64752.1"
                     /translation="MSRIHSRRIVPSSKRAQAAWVLYGALLGNLHDAGEAIERCAADR
                     GRDALFQSQALWESVLERLEEMSCNARELPAEAKAGMPRIDWCTWEHLPDTLAGSPRE
                     RRDRLTFVIDTLVPDTLVAAEDYRRALQPQASRA"
     gene            complement(3364..4857)
                     /gene="pepD"
                     /locus_tag="PLH85_09070"
     CDS             complement(3364..4857)
                     /gene="pepD"
                     /locus_tag="PLH85_09070"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HNB64753.1"
                     /translation="MNAPDRLDPVFDGLSPAPVWAHFAAFCRIPRPSRGEAAIREHLL
                     AWATARDIAAQVDAAGNLILRKPASPGHEGAPGVILQAHLDMVCQANAGTPHDFTRDP
                     IRPVRRDGWLVAEHTTLGADNGIGASLILAALEDDTLIHGPLEALLTVDEEAGMGGAH
                     GLEPGLLNGRLMLNLDTEDWGEFYLGCAGGLDVDARATGQADPLPAGWRSLRLSLAGL
                     RGGHSGVNIHEERGNAIKLLVRVLCDLASDAPLRLALLRGGTARNALPREASAVIAVP
                     AAVADGLPARVAAWQALLQDELRGVEAGLSLHLDDAAADSVLAVADQARWLNALHAAP
                     HGVRRWSLDVPGVVETSNNLGIVELTPNGGHANFMVRSLRDSGSAALAAELLSLLTLA
                     GLPATASGHYPGWTPNPDSPLLALCQRVFTRRFGGTSTVKVVHAGLECGLIGARYPGL
                     DTVSFGPTIRGAHAPGERVEIASVDAAWQLLGALLAALARGELPAPA"
     gene            5002..6039
                     /locus_tag="PLH85_09075"
     CDS             5002..6039
                     /locus_tag="PLH85_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNB64754.1"
                     /translation="MVRIAINGYGRIGRCFLRALRASPWRDALQVVAINEPADLDSMA
                     YLTRFDSTHGVFPGEVTVQDGGLSIDGQAIRVFHADTPEGVAWAEAGVDLLVEASGRY
                     STRAELERFVAAGCPRVLLSHPGHSAADVDRTIVYGMNHALLRGDERLVSAASCTTNA
                     IVPVLALLDEAFGIEHALMTTLHSVMNDQPLIDGYHHADLLRTRSAMQSLIPVSTGLA
                     RGVQRLLPELAGRIEAKAIRVPVVNVSAIDLVATLRRDADRGAVQALLRDAAAGPGAG
                     RMAYTDEPHASIDFNHSPHSAIVDGGQIRLVGGRLLNLLVWFDNEWGFANRMVELAGH
                     WARRMDAPGAG"
     gene            complement(6043..8073)
                     /locus_tag="PLH85_09080"
     CDS             complement(6043..8073)
                     /locus_tag="PLH85_09080"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNB64755.1"
                     /translation="MNAIPASAYPRLLAPLDLGFLTLRNRVLMGSMHTGLEEGEDPHA
                     RLAAFYAARARGGVGLIVTGGYGPNPDGAIHAGGSVFDSEDEARRHRVITDAVHAEGG
                     RICLQLLHTGRYAYSRSPVAPSAVKAPINPATPRALDEDEILRTIADYARAATLARAA
                     GYDGVEVMGSEGYLINQFLAPGTNLRDDRWGGSFENRCRFGLEVLRAVRAAAGHDFIV
                     IFRLSLLDLVEGGSTWDEVLAFARAVEQTGVNFINTGIGWHEARIPTIMSSVPHGAFT
                     ALTARLRGVVNVPLITSNRLNDPATAEAVLARGDADMVSMARPFLADADFVAKAAAGR
                     ADTINTCIGCNQACLDHIFAGKTSSCLVNPAACREWEGPAAPARTPRRVAVVGAGPAG
                     LAAATTLAERGHEVSLFDAADTVGGQFNLARRIPGKEDFGETLRYFRVRLAELDVALR
                     LGHTVTAADLAGFEHVVLATGITPRAPAIPGVDHPKVTSYIDVLEGRRSVGPRVALIG
                     AGGIGHDVAEFLTHAHDGSDPITAYRNEWGIDAAYTDNRGGLTAPRGPAVPRQVWLLQ
                     RKASRVGAGLAKTTGWARSLLLRKRGVHMLGGVAYLRIDDAGLHLRVGDADQVLDVDT
                     VVICAGQEPRRDLAAGLEAAGIPYTLVGGADVAAELDAKRAIAQATEFARRF"
     gene            complement(8332..8829)
                     /locus_tag="PLH85_09085"
     CDS             complement(8332..8829)
                     /locus_tag="PLH85_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNB64756.1"
                     /translation="MRIPSAPSLLFALLAGSLALPALADMPCGPMGGPMAPPGASRDN
                     RMERHTLHHAHLHEALKLTPEQEPAWQALMASEPTPMPAPTEDWRSLPTPERAERMLT
                     RMKAHEAQMSAHVDALKTFYATLTPEQKKTFDAAHAVGPRGMKGRMGPGMMGGGTGGR
                     MSDRP"
     gene            complement(9022..9885)
                     /locus_tag="PLH85_09090"
     CDS             complement(9022..9885)
                     /locus_tag="PLH85_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNB64757.1"
                     /translation="MNTRTHRSPLRASLALCALGLLTGTARADVRPDPGDYTGLPAGT
                     NLLLGYAQFPRADKVYSHGNQTVSNLDLSINAGIFRYVHFMKLGDYIIDPQIIVPFGT
                     QKIGLSDSKTSGLGDVIFGGTLWTLADLTRGEHLGWSVFFTAPTGSDRNQGFALSNNR
                     WATDLQVGYIRKLSDTWTLDLIGETEFYQDQRDTKARKDPLYQGHAHLRYFLSGATYL
                     AGTYRHAWGARESLNGADIAGRKNNGTAMLTWASFVDKQWQVQLQYTQDVKVEDGPKV
                     NGVQARVLYVY"
     gene            10359..12305
                     /locus_tag="PLH85_09095"
     CDS             10359..12305
                     /locus_tag="PLH85_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNB64758.1"
                     /translation="MGEMQRAFDARMLRARRAFFEDGTRPSGVIPDAVLRSWERSRQQ
                     GLGVRDRRIFMPVSREEVGRLQAENSELMACALPEMERLQAALGRGGWGIVCTDAQAR
                     VLKAVYGRSTAFREMDRIFEIGRNLSESAIGTNGPGCALIEGRPLTVRAAEHFLDETR
                     PFTCASVPLFGPDGRIVGVLDATRYCDGAAPTVVEPLAIAARAVENRLLGRLGAALKI
                     AFHCREDMLGTPMEGVLVFDGDGVLRGANQAARQLLSLTAGDDGGVRFDSVFDCPPET
                     FGRCLGGRVERTVLWGGEGMRFELRVEAAQPRPVLARPASVPSRAGSGWASAPSAACT
                     RDDGEDAAVARALHVAQRAFERDIPVLVLGETGTGKEVLARRLHGDSARADGPFVAIN
                     CSSLPAGLIESEFFGYEDGAFTGGRRGGAPGKFELAQGGTLFLDEIGDMPLELQGRLL
                     RVLQERNFSRLGGARLIHFDARIVAATHRKLDEAVAAGAFREDLYYRIKGVRVRLPAL
                     RERADRAALIDTLLRRECGGRRPPPLSAEVRAVLLGYRWPGNLRQLGHVLRLALTLAE
                     DDGEIGLQHLPEELLEAGSPTGGGLAPDLREQCLREVELDAVRAAMARCGGNVSAAAR
                     SLGVARATLYRKLRMLGMVGEGEA"
     gene            12804..13454
                     /locus_tag="PLH85_09100"
     CDS             12804..13454
                     /locus_tag="PLH85_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB64759.1"
                     /translation="MSESSPPRHQRRKEARPAELAAAALSLFVEKGFAATRLDEVAAR
                     AGVSKGTLYLYFDSKEALFRAVIEGSVVPLVEQSEARIEALKHDPERLLRETLVNWWF
                     NFGATELGGICKLMISEAGNFPEVAAYYNEAVIVRWMNLLGRVLELGMEQGVFRRLNV
                     DILRQQVFFPLLMQSVWKNSLAGCCAPVALPDSYFDTYFDLVFRGLLAAPPLEPSR"
     gene            13451..14542
                     /locus_tag="PLH85_09105"
     CDS             13451..14542
                     /locus_tag="PLH85_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB64760.1"
                     /translation="MMRPLVLAVAVATVLAACSKPEPVAEAPRPVVAQKLTPVAVASG
                     ALYTGEVRARYESDLAFRITGKIVERKVDVGAVVKPGQVLARLDPADAGLNAEAARAQ
                     LAAAQTDLAFAKAEVERYRDLVAKHFVSRSVLDAKEATFNAAAARVAQARAQASVAGN
                     QAGYTTLVADQAGVITAILAEAGQVVKDSAVVMRLARDGEREVLIAVPESRVAALRKA
                     GQVEIRLWAQPERALEGRVREIAPSADAATRTYAVKVTILSNDPTVQLGMTANVILPD
                     AAAGALLVPSTAVFEHAGGSAVWVLQADGELVKPVLRPVTVKQYREDGVILSGGVQAG
                     ETVAVAGVHKIVAGQPLRPVAPARPGAGA"
     gene            14539..17670
                     /locus_tag="PLH85_09110"
     CDS             14539..17670
                     /locus_tag="PLH85_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB64761.1"
                     /translation="MKRFNLSEWALRHQALVRYLIVALALVGVMAYQRLGQSEDPPFT
                     FKVMVVRTEWPGASAREIEEQVTDRIEKKLQEVPFVDYLRSYSRPGESVVLFVAKDSA
                     PASAIPDVFYQVRKKIGDIKGMLPTGIRGPYFNDEFGDVFGNIYALTGDGFDYAALKS
                     ASETIRAELLRVPNVAKVDLFGEQEQRIYVELSNTKLATLGLDVQTVTAALAQQNTVA
                     PGGYFETRDDRIYLRPSGAYDTVEAIRETRIRAGGREFRIGDIATVARGFVDPPAPRF
                     RYRGHDGLGLGVSMAKGGDIIALGQNLKEAVARIQAQLPVGMELAEVASQPEAVQRSI
                     SEFVHSLAEAVIIVLVVCFFSLGLRTGLVVALSIPLVLAVTFFGMRLFDVGLHKISLG
                     ALILALGLLVDDAIIAVEMMLVKMEQGWERARAAAYAYTSTAGPMLSGTLVTVAGFLP
                     IATAQSSTGEYTRSIFQVNAIALLASWLAAVIVVPYLGYVLLPDPRKAAGPGWLERRV
                     PAFARLKARLGIGGHTHHDGDEDAVFQTPFYNRFRALVDRCVARRWWVIGATLAIFVL
                     ALVGFGQVQQQFFPNSTRLEILVDLRLPEGSSLRATQAEAARLEAILDREPGIANYVT
                     YVGTGSPRFYLPLDQQLPAANFAQLVILTDSIPAREALRQRLIELFRTDFPGLRANVT
                     RLENGPPVGFPVQFRVSGPDIPTVRRFAAEVAEVMRANPNLSDVQLDWDEQSKVLRVE
                     VDQHKARLLGLSTQDVANFLNTSLRGLTATSYREGIESIDVVLRGDAFERDKLSLVSE
                     LSIPTRAGKSVPLGQVATVSYAWEQGLIWRRDRLRSITARGQIYDSRLQAPTVTAQVE
                     PLLAPIKARLPAGYRIDTGGAVEESAKGQSSVNAGFPLFFAVVFTVLMVQLNSFSRTT
                     MVVLTAPLGLIGVVLFLLLFNQPFGFVAMLGTIALFGMIMRNSVILVDQIEQDVEKGR
                     SVWNAIVESAVRRLRPIVLTAAAAVLAMIPLTRSAFFGPMAVAIMGGLIVATALTLLF
                     VPALYAAWYRVRR"
     gene            17774..18427
                     /locus_tag="PLH85_09115"
     CDS             17774..18427
                     /locus_tag="PLH85_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNB64762.1"
                     /translation="MNFEQARFNMVEQQIRPWEVLDFSVLDLLMSIRREEFVPEAYKS
                     VALSEAPIPLGHGASMLVPVIEGKVLQAIQVKGSDKVLEVGAGSGYFAALLAAKAEWV
                     RTVEIEPALVNMAHDNLKRYGVENVIVEEGDAVSGWAGNAPYDLIVVSGAVPHVPESL
                     LAQVKVGGRLFAFVGGPTLQVATLIQVVGEGQYRTEKVFETQVPMMRNAPRKNQFQF"
     gene            18440..18763
                     /locus_tag="PLH85_09120"
     CDS             18440..18763
                     /locus_tag="PLH85_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB64763.1"
                     /translation="MQQILPAQLDEWLRDASRPQPVLLDVREPHEFQYCALAGSLSMP
                     MASVPARQQELDPDAETVVICHHGMRSMQVALFLERQGFGKVINLTGGVALWAQQVDP
                     SMPQY"
     gene            18782..20101
                     /locus_tag="PLH85_09125"
     CDS             18782..20101
                     /locus_tag="PLH85_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNB64764.1"
                     /translation="MSLSKVRFAVLAWAGVMASGAQAVDLEQAYRDAVTYDATLSGAR
                     ASLAAGREKLPQGRAGLLPTISLSGNTNYNTAVFNNLDPHFTSKYNSHSWIAQLNQPL
                     FRWQNWVQFKQGELNVGLAEAQFKLAEQDLVLRVAQAYFDVLAAQDTLVAASALRDAN
                     AQQLALAKKSFEVGTVTITDVHEAQSRYDLSSAQTIAAESDLEVRRNALAVLTGKTPD
                     ALKTLRKGVALSSPEPADMPSWVSASEATALQVQAAQLGVEIAAREIERNRAGHAPTV
                     DLVASYGNSVNPVASVTPTTNYDSRAIGLQVAIPLYQGGGVNSRVREAIALRDKAAAD
                     LDNARRTAAQAARQAYLGVTSGLAQVRALEAAQVSSRSALDANKLGYEVGVRINIDVL
                     NAQSQVFDTEQKLSKARYDTLMAQLKLKAAAGTLGDDDVRALNALLD"
     gene            20237..21709
                     /gene="ubiD"
                     /locus_tag="PLH85_09130"
     CDS             20237..21709
                     /gene="ubiD"
                     /locus_tag="PLH85_09130"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HNB64765.1"
                     /translation="MRYTDLRDFIAQLERLGELKRVRVPVDPHLEMTEICDRVLRAGG
                     PAILFEQPTGHSVPVLANLFGTPQRVALGMGESGADWQSALREVGKLLAFLKEPEPPR
                     GLKDAWDKLPVFRQVLNMSPKTVSSGPCQEVVREGRDVDLASLPIQHCWPGDVAPLIT
                     WGLVVTRGPHKPRQNLGIYRQQVLGPNKVIMRWLAHRGGALDFRDHQQAHPGEPFPVA
                     VVLGCDPATILGAVTPVPDSLSEYQFAGLLRGAKTELVKCLGSDLQVPASAEIVLEGV
                     IHPGETALEGPYGDHTGYYNEQAEFPVFTIERITHRRDPIYHSTYTGKPPDEPAMLGL
                     ALNEVFVPLLQKQFPEIVDFYLPPEGCSYRLAVVSIRKQYPGHAKRVMFGIWSFLRQF
                     MYTKFIIVVDDDVNIRDWKEVIWALTTRMDATRDTTLVDHTPIDYLDFASPVAGLGSK
                     MGLDATNKWPGETQREWGRAIVMDAAVKARVDALWAQLGL"
     gene            21766..23358
                     /locus_tag="PLH85_09135"
     CDS             21766..23358
                     /locus_tag="PLH85_09135"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB64766.1"
                     /translation="MLCRMLARALLSAGVILMPAFASADLVGAGAVQFETDRLAEGQA
                     ALRSRQLERAERAFSEALRANAGSTDALIGLAQTAQLQGRPDKARDWMAKAVAAAPGS
                     PPILQAQSKLLVEQGMGALAEEHYRRAIAANPGNPQFSQDLASLYLEQLKRPAAAVTV
                     LRDLLRRQPELASAYLGLGLALAADGKLDEAGRTLDEAVQRDPVNPFAYHAQGLLALK
                     QGRADAALTAFDKALALRPEFVGALLGRGDALLALGKADGALATYRRAASLAPGSAVP
                     IAKRAQALERLKRPDEAEAAYREALKLEPTHTVVLNNLAFLLASRKQRLDEALGLAQR
                     ATTLDPARADHFDTLGVVLLARGDQAGARKAFERAVAVDPAHAGARQHLARVGAPAEA
                     PAAAGPSAPAASPREIPKEAARSSTDDAARALSTRLEAWRQAWESKDAGRYLSFYGKD
                     FAPPDGRDRGTWEAERRVRLDKKGEIHVQIVAPELRVEGDTAAVSFEQRYRAGALVDT
                     GRKRMAWHREGGEWMIRREGMQ"
     gene            complement(23371..24552)
                     /locus_tag="PLH85_09140"
     CDS             complement(23371..24552)
                     /locus_tag="PLH85_09140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB64767.1"
                     /translation="MFIRIDQLIASPRRLLSAIVLFVVLDLAVLLINVWIAAQVAEDA
                     VAINLAGRQRMLSQEITKALLLAVHVPSADARRDAEQELRTAYRLFERTLRAFHDGGD
                     TAGGTGPSVHLRPIDPGAGLDAVERVATLMAPLDARLPEFLAGKPLADSELGEATHYM
                     VQHNRDILAEMNRLTSALEQASVQRTRTLRSIQIGAFVLALGNFTVIVVGLVRRYRNL
                     TQESQRWRRLAEFDPMTGLASRTAYREARDSTHDAAVRWQRSATLLLIDLDGFKPIND
                     TFGHAVGDLLLAAVSERLRQVARNTDLVGRLGGDEFALICLDISAENDIGPLCERLLA
                     EIAALTTEDGRRYGVTGSIGAAVIPGPYPSADAWEAAADAAMYAAKRRGGNAWRLASA
                     V"
     gene            complement(24694..25878)
                     /locus_tag="PLH85_09145"
     CDS             complement(24694..25878)
                     /locus_tag="PLH85_09145"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNB64768.1"
                     /translation="MQVKKLTDLDVKGKKVFIRADLNVPQDDAGNITEDTRIRASIPS
                     IRYAVEGGAAVMVTSHLGRPTEGELKAEDTLAPVAVRLGQLLGKPVRLIQNWVDGGFD
                     VAPGEVVLLENCRCNKGEKKDNEELARKMAALCDVYVNDAFGTAHRAEATTHGIARFA
                     PTACAGILMGAEIDALGKALHSPKRPLVAIVGGSKVSSKLTILKSLADKVDQLIVGGG
                     IANTFLLASGKRIGESLAEPELVKEAQVIIDLMKARGAEVPLPEDVVVADEVSAMARA
                     NKVPVDEVGPHDRILDIGPKAAAQLSQIIANAGTIVWNGPVGVFELPQFAGGTKMMAS
                     AIAHSEAFSIAGGGDTLAAIAKFHIADDVGYISTGGGAFLEFLEGKTLPAIAALEERF
                     KD"
BASE COUNT         3896 a         8617 c         9381 g         4137 t
ORIGIN      
        1 cgtagccgcc gatgatcacc atgatcagca ggttggcgat catcaccacg tcgatgaggc
       61 ccagcaccac cagcatggtc tcggtctcgc tgatgtggcc gccggattcg aagaccgagc
      121 cgatcaggtg ggccagttcg gccatgaaca gatagacata gacgccttgc gcggcgacca
      181 gccccaggta gaggggcgcc tggaaccagc ggctccagaa gatgaagcgt tcgaggtgct
      241 gttggcctgg actcatggga aaaactcgga gtgaaggacg gcggatttta tcatcggcgc
      301 aggcgcttcc gagacgttaa aatgtcggac tttgcttttc cgcctcaagg gctttccgca
      361 tgaacctgca ccccatcacc gcgctgtcgc ccctggacgg ccgctacgaa tccaaggtcg
      421 ccgccctgcg cgagcacttc tccgaattcg gcctgatccg caaccgggtc aaggttgaga
      481 tcgagtggct caaggccctc gccgccgcgc ccgaactcgc cgagatcgcg cccttttccg
      541 ccgccaccgt ggccgagctg gacggcgtga tcgcccactt ttcggtgacc gacggcgagg
      601 ccgtgaaggc catcgaagcc accaccaacc acgacgtgaa ggcgatggag tactggctca
      661 agcagcgcct gggccacaac ccggaagtca cccgcgtcac cgagttcatc cacttcggct
      721 gcacctcgga agacatcaac aacgtctccc acgccctcat gctgcgcgac ggccgcgacg
      781 gcgtgctgct gccggccctc gacaggatca tcgcccgcct cgtcgagctg gcccacaacc
      841 tggccgacct gcccatgctg tcgcgcactc atggccagcc cgccagcccg accaccctgg
      901 gcaaggagat ggccaacgtg gtgcaccgcc tgcgccgcgt ccgcgcgtcc atcgccggcg
      961 tcgagctcac cgccaagttc aatggcgccg tgggcaatta caacgcccac ctgtccgctt
     1021 accccgactt cgactgggaa ggcttcaacg gccgcttcat cgagtccctg ggcctgacgt
     1081 tcaatcccta tacgatccag atcgagcccc atgacgcgat ggccgagctc tacgatgcca
     1141 cggcccgcgc caacaccatc gtcctcgacc tgtgccgcga catctggatg tacatctccc
     1201 tgggttactt caagcagaag ctcaaagccg gcgagatcgg ctcctccacc atgccccaca
     1261 aggtcaaccc catcgacttc gagaacgccg aaggcaacct gggcctggcc aacggcgtgc
     1321 tgcgccacct ggccgacaag ctgccggtgt cgcgcatgca acgcgacctc accgactcca
     1381 cggtgctgcg taacatgggc gtggccttcg gctacgcggt gctggcctac gattcctgcc
     1441 tgcgcggcct gggcaagctg gaggccgatc cggcccgcct cgccgccgat ctggacgcct
     1501 gctgggaagt gctcgccgag cccatccaga ccgtcatgcg ccgctacggc gtgcccaacc
     1561 cctacgagca gctcaaggag ctgacccgcg gcaagggcat cacccgcgac gcgctgcagg
     1621 ccttcatcgc caccctggcg attccggagg ccgaaaagac ccgcctgctc gccatgacgc
     1681 ccgccagcta catcggcaag gccgccgagc tcgcccgccg catctgaacc ccgtacgggc
     1741 tgccgtatgc gccgcgtgcg cagcccgacg accggagacc tggaatggct ttcgccgaca
     1801 aactgaagac cctgcccggc gtgtctcacc tggcagcgct gcacctcctc gacggcgctg
     1861 gccaggtggt tggcaccatc gagaacaagc ccggccaggc cggctccctg gcggtgtaca
     1921 accacctggc ccagcttcac ggcgccatca ccccggacgc cgccacccag ggcatcgagc
     1981 tctacgccga gcacagcgag gatgcgcgcc tcaaccctgg caagcacccc aacatcgacc
     2041 gcctgatcgg tctggtggag cggggcgaga cgctgcgggt caagcacgtc ttcgccgcct
     2101 gagcgctgcg cgcgctggca tggcgggccg cctgcgggcg gcctttttcg tggatgcggg
     2161 gctcaggctc tggctatatt caccacgcga cgggcaaacc agccggccag acgcagtccg
     2221 atgaacaccc cgaccgaatc cgccagccag tcccacgggt cgccgacgcg attggtcgtg
     2281 aagcggtgct ggcacacttc gatcagcagg ccgtaggcca ccagcccggc ggccaccgcc
     2341 gtccggcgca gggggaaccc ggtcacgccc atcagggtca gcaggacgaa ggcgccggtg
     2401 tgttccacct tgtcctgaaa ggagaacacg gtcagggctt cgggcagcgg gatcatcgcc
     2461 agccagaaga tcacgaccag ggccagcgca aaggctgtgc ggacgacagg gggcggaaag
     2521 cgcaggggca tcggcagggc gaaggaaccg gggacggcgg attctagcgc ggacgcggcg
     2581 ccggtcgccc gaccgttctc atggcatctg tcacagggtg ttcacgcgga cggtgttaga
     2641 tgacgcccca atgctcatga acgctgcaaa gcaccgctga aatgatgggt ttgtactgtc
     2701 tttcggcggg cctacactgc ctgggcggca ccgcgtggac gccgcctgtc gcgagcggag
     2761 ggcgatgcag tgacgatgtc atctgcgcag tgacaaatgt caccgccgtc cagtgacggc
     2821 tgacctatcc tgccagcccg gttctccggc acactgccgc tcaaccggaa atcagggtga
     2881 ccggcgtctg ccggtacccg gccggaacct cgaaggcccg gtgcaactcg aaggtgtccc
     2941 atgtcccgaa tccattcccg tcggatcgtc ccgtcgtcca agcgcgccca ggccgcctgg
     3001 gtgctctatg gcgccttgct gggcaacctg cacgacgccg gggaggctat cgaacgctgc
     3061 gccgccgacc gggggcggga cgccctgttc cagtctcagg ccctgtggga gtccgtgctg
     3121 gagcgcctgg aggagatgag ctgcaatgcc cgcgaactgc cggctgaggc caaggcgggc
     3181 atgccgcgta tcgactggtg cacctgggaa cacctgccgg acaccctggc gggctcgcca
     3241 cgggagcgcc gcgaccgcct cactttcgtc atcgataccc tggtgccgga caccctcgtc
     3301 gctgccgagg actatcgtcg ggccctgcag ccgcaggcgt cccgcgcctg accccggcac
     3361 ccctcacgcc ggcgcgggca gctccccgcg ggcgagcgcg gccaggagcg cgcccagcaa
     3421 ctgccaggca gcatccaccg acgcgatctc cacccgttcg cccggcgcgt gggcgccgcg
     3481 gatggtgggg ccgaaggaga ccgtgtccag gcccggatag cgtgcgccga tgaggccaca
     3541 ttcgagcccc gcgtgcacca ccttcaccgt ggacgtcccg ccgaagcgac gtgtgaacac
     3601 ccgctggcac agggccagca gcggcgaatc cggattcggc gtccagcccg gatagtggcc
     3661 gctcgcggtg gccggcaggc cggcaagggt cagcaggctg aggagctcgg cggcgagggc
     3721 ggcgctgccg ctgtcgcgca gggagcgcac catgaaattg gcgtgaccgc cgttgggcgt
     3781 cagttccacg atgcccagat tgttggaggt ttccaccacg ccgggtacgt ccaggctcca
     3841 gcgccgcacc ccgtggggcg ctgcgtgcag ggcgttcagc cagcgggcct ggtcggcgac
     3901 cgcgagcacg ctgtcggcgg cagcgtcgtc aaggtgcagg ctgaggccgg cttccacgcc
     3961 gcgcagttcg tcctgcaaga gggcctgcca ggcggccacg cgcgcgggca gcccgtcggc
     4021 gacggcggcc ggtacggcaa tcactgcgct ggcttcgcgg ggcagggcat tgcgggcggt
     4081 gccgccgcgc agcagggcga gacgcagcgg cgcgtccgat gccaggtcgc acagcacgcg
     4141 gaccagcaac ttgatggcgt tgccccgctc ttcgtgaatg ttcacgccgg aatggccgcc
     4201 gcgcaggccc gccaggctca ggcgcaggct gcgccagccc gccggcagcg ggtcggcctg
     4261 gccggtggca cgggcatcca cgtccagccc gccggcgcag cccaggtaga actcacccca
     4321 gtcctcggta tccaggttga gcatcaggcg gccgttcagc aggccgggtt cgaggccgtg
     4381 ggcgcccccc atgccggcct cttcatccac cgtgaggagg gcttcgaggg ggccgtggat
     4441 cagcgtatcg tcctccagcg ctgccaggat cagcgacgcg ccgatgccgt tgtcggcgcc
     4501 gagggtggtg tgctcggcca ccagccagcc gtcgcgccgt acgggccgga tcggatcgcg
     4561 ggtgaagtcg tggggcgtgc cggcgttggc ctggcagacc atgtccaggt gcgcctgcag
     4621 gatcaccccc ggggcgccct cgtggcctgg gctggccggc ttgcggagga tcaggttgcc
     4681 ggcggcgtcc acctgggcgg cgatgtcgcg ggccgttgcc caggcgagga ggtgctcgcg
     4741 gatcgccgcc tcgccgcggg aggggcgcgg gatgcggcag aaggcggcga aatgggccca
     4801 cacgggggcg gggctgaggc cgtcgaagac ggggtcgagg cggtccgggg cgttcatggc
     4861 agggcttcgt ggggactggg agtgggcggg cgacaggccc gcgccggcgc cattatcgcc
     4921 cagcctgccg ggcagggacg ggccggaggg tgtaggcttg cgcctttttc agcagggcgg
     4981 aaaggcgtgc acggttcgga catggtgagg attgcgatca acggctacgg gcgcatcggg
     5041 cggtgtttcc tgcgggcgct gcgggcgtca ccgtggcggg acgccttgca ggtggtggcc
     5101 atcaacgagc cggcagacct ggacagcatg gcctacctga cgcgcttcga ctccacccac
     5161 ggcgtgttcc ctggcgaggt gacggtgcag gacggcggcc tgagcatcga cgggcaggcc
     5221 atccgcgtct tccacgccga cacgccggag ggggtggcct gggccgaggc gggcgtggat
     5281 ctgctggtag aggcctccgg gcgctacagc acgcgggccg agctggagcg cttcgttgcg
     5341 gcgggctgcc cgcgggtgct gctgtctcac cccgggcaca gcgcggcgga cgtggatcgc
     5401 accatcgtct atggcatgaa tcacgcgctg ctgcgcggcg atgagcgcct cgtgtcggcg
     5461 gcctcgtgca ccaccaacgc catcgtgccg gtgctggccc tgctggacga ggccttcggc
     5521 atcgagcacg cgctgatgac caccctccat tcggtgatga acgaccagcc gctgatcgac
     5581 ggctaccatc acgccgacct gctgcgcacc cgctcggcga tgcagtccct gattccggtg
     5641 tccaccggcc tggcgcgggg cgtgcagcgt ctgctgccgg agctggccgg gcgcatcgag
     5701 gccaaggcga tccgcgtgcc ggtggtgaat gtgtcggcca tcgacctggt ggcgaccctg
     5761 cgccgcgacg cggaccgtgg ggcggtgcag gccctgctgc gggacgccgc cgccggcccc
     5821 ggcgcggggc gcatggccta cacggatgag ccccacgcct ccatcgactt caaccacagc
     5881 ccccattcgg ccatcgtgga tggcggccag atccgcctgg tgggcgggcg gttgctcaac
     5941 ctgctggtgt ggttcgacaa cgaatggggc ttcgccaacc gcatggtgga gctggctggg
     6001 cactgggccc ggcgcatgga cgcgccgggc gccggctgag cgtcagaaac ggcgggcgaa
     6061 ctcggtggcc tgggcgatgg cgcgcttggc gtccagctcg gccgccacgt cggcgccgcc
     6121 caccagggtg tagggtatgc cggcggcctc caggccggcc gccagatcgc ggcgcggctc
     6181 ctggccggcg cagatcacca ccgtatccac gtccagcacc tgatccgcat cgcccacgcg
     6241 caggtgcagg ccggcgtcgt cgatgcgcag atacgccacg ccgcccagca tgtgcacgcc
     6301 gcgcttgcgc aggagcaggc tgcgcgccca gccggtggtc ttggccaggc ccgcgccgac
     6361 gcggctggcc ttgcgctgga gcagccagac ctggcgcggc acggccggcc cccggggcgc
     6421 ggtgaggccg ccccggttgt ccgtgtaggc ggcgtcgatg ccccattcgt tccggtaggc
     6481 ggtgatgggg tcgctgccgt cgtgggcgtg ggtgaggaac tccgccacgt cgtggccgat
     6541 gccgccggcg ccgatcaggg cgacccgcgg gcccacgctg cggcggcctt ccagcacatc
     6601 gatgtagctc gtcaccttgg ggtggtccac gccgggaatg gcgggcgcgc gcggcgtgat
     6661 gccggtggcc agcaccacgt gctcgaagcc cgccaggtcg gcggccgtca cggtgtggcc
     6721 caggcgcagc gccacgtcca gctcggccag gcgcacgcgg aaatagcgca gggtctcgcc
     6781 gaaatcctcc ttgccgggga tgcgccgggc caggttgaac tggccgccga cggtgtcggc
     6841 cgcgtcgaac aggctgacct cgtggccgcg ttcggcgagg gtcgtggcgg cggccagacc
     6901 ggccgggccg gcgcccacca cggccacccg gcgcggcgtc cgggccggcg ccgccgggcc
     6961 ttcccactcc cggcaggcgg ccggattgac caggcaggac gaggtcttgc cggcaaagat
     7021 gtgatccagg caggcctggt tgcaaccgat gcaggtgttg atggtgtcgg cgcggccggc
     7081 ggcggccttg gcgacgaagt ccgcatcggc caggaagggt cgggccatgg acaccatgtc
     7141 cgcgtcgccg cgggccagta cggcctcggc ggtggccggg tcgttgaggc ggttgctggt
     7201 gatcaggggt acgttcacca caccccgcag gcgtgcggtg agggcggtga aggcgccgtg
     7261 gggcacgctg ctcatgatgg tggggatgcg cgcctcgtgc cagccgatgc cggtgttgat
     7321 gaagttgacg ccggtctgct cgacggcgcg ggcgaaggcc agcacttcgt cccaggtgct
     7381 gccaccttcc accaggtcca gcagggacag gcggaagatg acgatgaagt cgtgtccggc
     7441 ggcagcgcgc acggcgcgca ggacctccag cccgaagcgg cagcggttct cgaaactgcc
     7501 gccccagcgg tcgtcccgca gattggtgcc gggggcgagg aactggttga tcaggtagcc
     7561 ctcggagccc atcacctcga ccccgtcgta gccggcggcg cgggccaggg tggcggcgcg
     7621 ggcgtagtcg gcgatggtgc gcaggatctc gtcctcgtcg agggcgcggg gcgtggccgg
     7681 gttgatgggg gccttgacgg ccgacggggc cactggcgaa cggctgtagg cgtaacggcc
     7741 ggtgtggagc agttgcaagc agatccgccc gccttcggca tggacggcgt cggtgatgac
     7801 gcggtggcgg cgggcctcgt cctcgctgtc gaacaccgag ccgccggcgt ggatcgcgcc
     7861 gtccgggttg gggccgtagc cgccggtgac gatgaggccg acgccacccc gcgcgcgggc
     7921 ggcgtagaag gcggcgaggc gtgcgtgggg atcttcgcct tcctccaggc cggtgtgcat
     7981 ggagcccatc agcacccggt tgcgcagggt gaggaagccg aggtcgaggg gcgcgagcag
     8041 gcgcgggtag gcagacgcag ggattgcgtt catgaagggc tgtctcctgg aattgtgctt
     8101 ggtgttgtgc cgcgcattgc aggggcgggc gcgggaaaag tcaagttgcc gtgacggcag
     8161 tcggggcggg gccaggagcg cggtgcgtgc gggcgcgggc aggacgttcg acatcgcggc
     8221 gggaagcgcc acgccgccgg gatctggcgg acggagacgt tgggggccgg tcggcgtgtg
     8281 gggagcgacc tgccgggacc gggccggcgg cggcccggtc tgctgcgacg atcaggggcg
     8341 gtcggacatg cggccgccgg tgccgccgcc catcatgccg ggacccatgc ggcctttcat
     8401 gccgcgggga ccgacggcgt gggcggcgtc gaaggtcttc ttctgctcgg gggtgagcgt
     8461 ggcgtagaag gtcttgaggg cgtccacatg ggcagacatc tgggcttcgt gggccttcat
     8521 gcgggtgagc atgcgctcgg cccgttcggg cgtggggagg ctgcgccagt cttcggtcgg
     8581 ggcgggcatc ggggtgggct cggacgccat cagggcctgc cacgcgggtt cctgttccgg
     8641 cgtgagcttg agggcctcgt ggaggtgggc gtggtgcagg gtgtgacgtt ccatgcggtt
     8701 gtcgcgggac gcgccgggtg gggccatcgg accccccatg gggccacagg gcatgtctgc
     8761 cagggcgggg agggcgaggc tgccggcaag cagggcgaac agcagggatg gggcggacgg
     8821 gatacgcatg aggactctcc gggtgcaaga cctgacgact ttacgccgat ccgtggcgcg
     8881 tgggcgatgc gcaggggggc gccgggtgca gccgggcccg gctgcgcgga gggcggtgcg
     8941 cgctaacggc ggaatgccga ctggtcgcgc cggggtggat cggtgcggac gcccggagcg
     9001 gggctccggg ccctgcgatc ctcaatacac gtacagcacg cgcgcctgga ccccattgac
     9061 cttgggcccg tcctcgacct tcacgtcctg ggtgtattgc agttgcacct gccattgctt
     9121 gtccacgaag gacgcccagg tcagcatggc cgtcccgttg ttcttgcggc cggcgatgtc
     9181 ggcgccgttg agggattccc gcgcgcccca ggcgtggcgg taggtgccgg ccaggtaggt
     9241 ggcgccgctc aggaagtagc gcaggtgggc gtggccctgg tacaggggat ccttgcgggc
     9301 cttggtgtca cgctggtcct ggtagaactc ggtttcgccg atcaggtcga gggtccaggt
     9361 gtcggacagc ttgcggatgt agcccacctg caggtcggtg gcccagcggt tgttgctgag
     9421 ggcgaagccc tggttgcggt cgctgccggt aggggcggtg aagaacaccg accagcccag
     9481 gtgttcgccg cgggtcaggt cggcgagggt ccacagggtg ccgccgaaga tcacgtcgcc
     9541 caggccgctg gtcttgctgt cggagaggcc gatcttctgg gtgccgaagg gtacgatgat
     9601 ctgcggatcg atgatgtaat cgccgagctt catgaagtgg acgtaacgga agatgccggc
     9661 gttgatggac aggtccagat tgctcacggt ctggttgccg tggctataga ccttgtctgc
     9721 ccgcgggaac tgggcgtaac ccaggagcag attggtgcca gcgggcagcc cggtgtagtc
     9781 gcccggatcg gggcggacgt cggcgcgggc cgtgccggtc agcaggccga gggcacacag
     9841 ggccaggctg gcgcgcaggg gggagcgatg ggtgcgagtg ttcatggaca gtctccgtgt
     9901 cgttgtggtg tgggtgctgc gatgcgctta aaaaagggcc gcggcgtccc ggagggaggg
     9961 gcgggggccg cggcaagggg aagcgggccg gctgggggca gcgtgaccgc cagtcgattc
    10021 agggggcacc accagccggt cgcggggagg gctgctcgga cactgcgcgc ggtggcttca
    10081 tgggggctcc cgattgactt tgtctggacg gattctttaa tgtgaaacgg acttggcgcg
    10141 gtggcagaac cggcgccccg gatcgtccgg gaaggatctg cacccttcgt gccaagcgct
    10201 gaacggcccc gcaagagcgc cgtttttcat aaaaaaacat caggttgggg cgatcggccg
    10261 ggtgcttgca cgcggagcgt ggggcgcgac gtcgacttga aacgagtcaa ccggaagttc
    10321 gcggttgact cgttccgcgc cgccgggagg agaccgccat gggtgagatg cagagggcgt
    10381 tcgacgcgcg catgctgcgc gcgcggcgcg cgttcttcga ggacggcacg cgtccgtccg
    10441 gggtgattcc ggatgccgtg ctccggtcct gggagcgctc ccgccaacag ggcctgggtg
    10501 tgcgcgaccg gcgcatcttc atgccggtga gccgcgagga ggtcggccgg ctgcaggcgg
    10561 aaaacagcga gctgatggcc tgcgccctgc ccgagatgga gcgtctgcag gcggccctgg
    10621 ggcgcggcgg gtggggcatc gtgtgcacgg acgcgcaggc gcgggtgctc aaggcggtgt
    10681 atggccgcag caccgccttc cgtgagatgg accggatctt cgagatcggc cgcaatctca
    10741 gcgagtcggc gatcggcacc aacgggcccg gttgcgcgct catcgaggga cggccgctca
    10801 cggtgcgcgc cgcggaacat tttctggacg aaacccggcc cttcacctgt gcatccgtgc
    10861 ccctgttcgg gccggacggg cgcatcgtcg gcgtgctgga cgcgacgcgc tattgcgatg
    10921 gtgcggcgcc cacggtcgtc gagccgctgg ccatcgccgc ccgtgcggtg gaaaaccgcc
    10981 tgctggggcg tctcggcgcc gcgctgaaga tcgcctttca ctgtcgcgaa gacatgctcg
    11041 gcacgccgat ggagggtgtg ctggtgttcg acggggacgg cgtgctgcgc ggggccaacc
    11101 aggcggcacg gcagctgctg tcgctgacgg cgggggatga cggaggcgtg cgcttcgaca
    11161 gcgtgttcga ctgcccgccg gagacctttg ggcggtgtct gggcgggcgg gtcgaacgga
    11221 cggtcctgtg ggggggcgag ggcatgcgct tcgagctgcg ggtcgaggca gcgcagccgc
    11281 ggcccgtact ggccaggccg gccagtgtcc cctcgcgcgc cggctcagga tgggcctccg
    11341 cgccgtccgc cgcctgtaca cgggatgacg gcgaggacgc cgccgttgcg cgggccttgc
    11401 atgtggcgca gcgggccttt gagcgggaca tcccggtgct ggtgctgggc gagaccggca
    11461 ccggcaagga ggtcctggcc cggcgcctgc acggcgacag cgcgcgtgcc gatggcccct
    11521 tcgtggcgat caactgctcg tccctgcccg ccgggctgat cgaatccgag ttcttcggct
    11581 acgaggacgg ggcgttcacc ggtggccgtc gcggcggtgc gccgggcaag ttcgaactgg
    11641 cccagggcgg caccctgttc ctcgacgaga tcggcgacat gccgctggag ttgcaggggc
    11701 gcctgctgcg cgtgctccag gagcgcaact tctcgcgcct gggcggggcg cggctgatcc
    11761 acttcgacgc ccgcatcgtg gccgccaccc accgcaagct ggacgaagcg gtggccgccg
    11821 gggcgttccg ggaagatctg tattaccgca tcaagggcgt acgcgtgcgc ctgccggcct
    11881 tgcgcgagcg ggccgaccgt gcggcgttga tcgatacgct gctacgccgg gaatgcggcg
    11941 gccggcgccc gccgcccctg tcggccgagg tccgcgccgt actgctcggc taccgctggc
    12001 cgggcaatct ccgccagctc ggccacgtcc tgcgtctggc cctgaccctg gccgaggacg
    12061 acggcgagat cggcctccag catctgcccg aggagctcct ggaggccggg tcgccgacgg
    12121 gcggcggtct ggcgccggac ctgcgggagc agtgcctgcg ggaggtggag ctcgacgccg
    12181 tcagggcggc gatggcgcgt tgcggcggga acgtctcagc ggcggcgcgc agcctgggcg
    12241 tggcgcgggc gaccctgtac cgcaagctgc ggatgctggg catggtgggc gagggggagg
    12301 cctgacgccg ccaaaattcc ccggcgccat tttttgatcc ggcggacgaa tttttaaatg
    12361 gctggcgggg atttaaaaat ccggcgcggg gatttttaag tgggatttcc ggatttcttt
    12421 ttgcggcggg cgcatttatc tgcctcattt tttggcaagc aaatccgtgg cgtgcatttt
    12481 ttaatctggc tgggggatct tgctgcctaa aaatgaggca gcaagatccg atgggcggat
    12541 ttttgtaggg tcgggatcct ttttttcacg gcaggcccgg ggcgcagcct cggtggcgcg
    12601 cgggccgatc ctggggcggt gcgcctgggt ggtgagtacg cacggaaagg gcgtgcccgg
    12661 cgttccggcg gcgccatcgg cgtgcggtga gccgcggccc cggtggcagg cgggaggagg
    12721 atccttcgac atcgcgcgcc gcctttactt ccgctcaagg ctgttctaaa ttgctgactg
    12781 aacggtcagt aattaatttt tccatgagcg aatcctctcc gccccgccac caacgccgca
    12841 aggaggcgcg ccccgccgaa ctggcggcag cggccttgtc cctgttcgtc gagaagggct
    12901 ttgcggccac ccgcctggat gaagtggcgg cccgcgccgg cgtgtccaag ggcaccttgt
    12961 acctgtactt cgacagcaag gaagccctgt tccgggcggt catcgaaggc agcgtggtgc
    13021 ccctggtgga gcagagcgag gcgcgcatcg aggccctgaa gcacgacccg gagcgtctgc
    13081 tgcgggagac cctggtgaat tggtggttca acttcggggc caccgagctg ggcggcatct
    13141 gcaagctgat gatctccgag gccggcaatt tccccgaggt ggcggcctac tacaacgagg
    13201 cggtcatcgt gcgctggatg aacctgctcg gccgcgtgct ggaactgggc atggagcagg
    13261 gcgtgttccg tcgcctgaat gtggatatcc tgcgccagca ggtcttcttc ccgctgctga
    13321 tgcagtccgt gtggaagaac tccctggcgg gctgctgcgc gccggtggcg ctgccggaca
    13381 gctatttcga cacctatttc gatctcgtct tccgtggcct gctggccgcg ccgccgctgg
    13441 agccttcccg atgatgcgtc cccttgtcct tgccgtcgcg gtggcgacgg tccttgccgc
    13501 gtgcagcaaa ccggagcccg tggccgaagc gccccgtccg gtggtggcgc agaagctcac
    13561 gcccgtggcg gtggcctcgg gcgccctgta caccggcgag gtgcgggccc gctacgagtc
    13621 ggacctggcc ttccggatca ccggcaagat cgtcgagcgc aaggtggatg tgggtgccgt
    13681 ggtcaagccc ggtcaggtgc tggcgcggct cgatccggcc gacgcggggc tcaatgcgga
    13741 ggcggcgcgg gcccagctgg ccgcggcgca gaccgatctg gcctttgcca aggccgaggt
    13801 ggagcgctac cgggatctgg tggccaaaca tttcgtcagc cgcagtgtgc tggacgccaa
    13861 ggaggcgacc ttcaacgcgg cggccgcgcg cgtcgcccag gcccgcgccc aggccagcgt
    13921 ggccggcaat caggcgggct acaccacgtt ggtggcggac caggccgggg tgatcacggc
    13981 gatcctcgcc gaggcggggc aggtggtgaa ggactccgcg gtggtgatgc gtctggcccg
    14041 ggacggggag cgggaggtgc tcatcgcggt gccggaaagc cgtgtcgccg ccctgcgcaa
    14101 ggcgggccag gtggagatcc gcctgtgggc ccagccggag cgggcgctgg aaggccgcgt
    14161 ccgcgagatc gcgccgagcg ccgatgcggc gacgcggacc tacgcggtga aggtgacgat
    14221 cctgtccaac gatccgaccg tccagctggg catgaccgcc aacgtgatcc tccccgatgc
    14281 ggccgccggc gccctgctgg tgccgtccac ggcggtcttc gagcacgcgg gcggcagcgc
    14341 cgtgtgggtg ctgcaggccg atggcgagct ggtcaagccg gtgctgcggc cggtgacggt
    14401 gaagcagtac cgggaggacg gggtgatcct gtccggcggc gtgcaggcgg gcgagaccgt
    14461 cgcggtggcc ggcgtgcaca agatcgtcgc cggtcagccc ctgcgcccgg tggcgccggc
    14521 ccgtccgggg gccggggcat gaagcgtttc aacctgtcgg agtgggcgct gcgccaccag
    14581 gccctggtgc gttacctgat cgtcgccctg gccctggtgg gggtgatggc ctatcagcgc
    14641 ctggggcagt cggaggatcc gcccttcacc ttcaaggtca tggtggtgcg caccgaatgg
    14701 cccggcgcct cggcgcggga gatcgaggag caggtcaccg accgcatcga gaagaagctc
    14761 caggaagtgc ctttcgtgga ctacctgcgc agctattcgc ggccgggcga atccgtggtg
    14821 ctcttcgtgg ccaaggattc ggcgccggcg tcggcgattc ccgacgtgtt ctaccaggtg
    14881 cgcaagaaga tcggcgacat caaagggatg ctgccgacgg gcatccgcgg gccctatttc
    14941 aatgacgagt tcggcgacgt cttcggcaac atctacgccc tgaccggcga cggcttcgac
    15001 tacgcggccc tcaagagcgc gtccgagacg atccgcgccg agctgctgcg ggtgcccaac
    15061 gtggccaagg tggatctgtt cggcgagcag gaacagcgca tctacgtgga gctctccaac
    15121 accaagctcg ccaccctcgg gctggacgtg cagacggtca ccgcggccct cgcccagcag
    15181 aacacggtgg cgccgggcgg ctatttcgag acccgcgacg accgcatcta cctgcgcccg
    15241 agcggcgcct acgacaccgt cgaggcgatc cgcgagacgc gcatccgcgc tggcgggcgg
    15301 gaattccgca tcggcgacat cgccacggtg gcgcggggct tcgtcgatcc gccggcgccg
    15361 cgcttccgct accgtggcca cgacgggctg ggcctgggcg tgtccatggc caagggcggc
    15421 gacatcatcg ccctggggca gaacctcaag gaggcggtcg cgcgcatcca ggcccagctg
    15481 ccggtgggca tggagctggc cgaggtggcg agccagcccg aggcggtgca gcgctccatc
    15541 agcgagtttg tgcattccct ggcggaggcc gtgatcatcg tgctggtggt gtgcttcttc
    15601 tcactgggcc tgcgcaccgg gctggtggtg gccctgtcga tccccctggt gctggcggtc
    15661 accttcttcg gcatgcgtct gttcgacgtg ggcctgcaca agatctccct gggcgccctg
    15721 atcctggccc tgggcctgct ggtggacgac gccatcatcg ccgtggagat gatgctggtg
    15781 aagatggagc agggctggga acgggcccgc gctgcggcct acgcctacac cagcaccgcc
    15841 gggccgatgc tgtcgggcac gctggtgacg gtggcgggct ttctgcccat cgccaccgcc
    15901 cagtcgtcca ccggcgaata cacccgttcg atcttccagg tgaacgccat tgcgctgctg
    15961 gcgtcgtggc tcgccgcggt gatcgtggtg ccctatctcg gttacgtgtt gctgccggac
    16021 ccccgcaagg ccgccggacc gggctggctg gaacggcgcg tgccggcctt cgcacggctc
    16081 aaggcgcggc tcggcatcgg cggccacacg catcacgatg gtgacgagga cgcggtcttt
    16141 cagacgccct tctacaaccg cttccgagcg ctggtagaca gatgcgtggc gcggcgctgg
    16201 tgggtcatcg gcgcgacgct ggcgatcttc gtgctggccc tggtgggctt cggtcaggtg
    16261 cagcagcagt tcttccccaa ttccacgcgg ctggagatcc tggtggatct gcgcctgccc
    16321 gagggcagct ccctgcgcgc cacccaggcg gaggcagcca ggctggaggc gatcctcgac
    16381 cgggagcccg gcatcgccaa ctatgtgacc tacgtgggca ctggcagccc gcgcttctat
    16441 ctgcccctgg accagcaact gccggccgcc aactttgccc agcttgtgat cctcaccgac
    16501 agcatcccgg cgcgggaggc gctgaggcag aggctgatcg agctgttccg tacggatttc
    16561 ccgggtctgc gggccaacgt gacgcgcctg gagaacgggc cgccggtggg cttcccggtg
    16621 cagttccggg tgagcgggcc ggacatcccc acggtgcgcc ggtttgccgc cgaagtggcc
    16681 gaggtgatgc gcgccaatcc gaacctctcg gacgtgcagc tggactggga cgagcagtcc
    16741 aaggtgctgc gcgtcgaggt ggatcagcac aaggcgcgcc tgctggggct gtccacccag
    16801 gacgtggcca acttcctcaa cacctctttg cgcggcctca cggccaccag ctaccgggaa
    16861 ggcatcgagt ccatcgacgt ggtgctgcgg ggcgacgcct tcgagcggga caagctgtcc
    16921 ctggtgtcgg agctgtccat ccccacccgc gccggcaagt ccgtgcccct gggccaggtg
    16981 gcgacggtga gctacgcctg ggagcagggc ctgatctggc gccgtgaccg tctgcgcagc
    17041 atcactgccc gcggccagat ctacgacagc cgcctgcagg cgccgaccgt caccgcccag
    17101 gtcgagccgc tgctggcgcc gatcaaggcg cgcctgccgg ccggctatcg catcgacacc
    17161 ggcggcgcgg tagaggagag cgccaaaggg cagagctccg tgaatgcggg cttcccgctg
    17221 ttcttcgcgg tggtgtttac cgtgctgatg gtgcagttga acagcttctc gcggaccacc
    17281 atggtggtgc tcaccgcgcc cctcgggctg atcggcgtgg tgctgttcct gctgctcttc
    17341 aaccagccct tcggctttgt cgccatgctc ggcaccatcg ccctgttcgg catgatcatg
    17401 cgcaactcgg tgatcctggt ggaccagatc gagcaggacg tggagaaggg gcgcagcgtg
    17461 tggaacgcca tcgtggaatc ggccgtgcgc cgcctgcggc ccatcgtgct caccgctgcg
    17521 gcggctgtgc tggcgatgat cccgctgacc cgcagcgctt tcttcgggcc gatggcggtg
    17581 gccatcatgg gcggcctgat cgtcgccacc gcgctgacgc tgctcttcgt gccggccctg
    17641 tacgccgcct ggtatcgggt gcgtcgatga cgcgttcccg cgcaagtacg ctagaattgg
    17701 aaggtttttc cattcacgct ggcaggccga tcctcccgtc cgggccgcgg cggatcttta
    17761 caggggagtt ccaatgaatt tcgaacaagc gcgcttcaac atggtcgagc aacagatccg
    17821 cccctgggag gtgctggatt tctccgtgct ggacctgctg atgagcatcc gccgcgagga
    17881 gttcgtgccc gaggcctaca agtccgtggc cctctccgag gcgccgatcc ccctcggcca
    17941 cggcgcgagc atgctggtgc cggtcatcga gggcaaggtg ctgcaggcga tccaggtcaa
    18001 gggttccgac aaggtcctcg aagtgggtgc cggctccggc tatttcgccg ccctgctggc
    18061 cgccaaggcc gagtgggtgc gcaccgtcga gatcgagccc gcactggtga acatggccca
    18121 tgacaacctg aagcgctatg gcgtcgagaa cgtcatcgtc gaggaaggcg acgccgtgag
    18181 cggttgggcc ggcaatgccc cctacgacct gatcgtggtc tccggcgctg tgccccacgt
    18241 gcccgaatcc ctgctggccc aggtgaaggt gggtgggcgc ctgttcgcct tcgtcggcgg
    18301 gccgaccctg caggtcgcca ccctgatcca agtggtgggc gagggccagt accgcaccga
    18361 gaaagtcttc gagacccagg tgccgatgat gcgcaacgcc ccgcgcaaga accagttcca
    18421 gttctgagcg gacgcggcga tgcagcagat cctgcctgcg caactggacg agtggctgcg
    18481 ggatgcgtcc cgcccgcagc ccgtgcttct cgatgtccgt gagccccacg aattccagta
    18541 ctgcgccctc gccgggtccc tgtccatgcc catggccagc gtgccggcgc gccagcagga
    18601 gctcgatccg gatgcggaga ccgtcgtgat ctgccaccac ggcatgcgca gcatgcaggt
    18661 ggccctgttc ctggagcgcc agggcttcgg gaaggtgatc aacctgaccg gcggggtcgc
    18721 cctgtgggcc caacaggtcg acccgtcgat gccccagtac tgacatcacc aggtgaaggg
    18781 aatgagcttg agcaaggttc ggtttgcagt gctggcgtgg gccggcgtga tggcgtccgg
    18841 tgcgcaggcc gtcgatctgg agcaggccta ccgggacgcg gtgacctacg acgcaaccct
    18901 ctccggtgcg cgggcctccc ttgccgccgg gcgtgagaag ctgccccagg gccgcgccgg
    18961 cctgctgccg acgatctccc tgagcggcaa caccaactac aacacggcgg tcttcaataa
    19021 cctcgacccc catttcacca gcaagtacaa cagtcacagc tggatcgccc agctcaacca
    19081 gcccctgttc cgctggcaga actgggtcca gttcaagcag ggcgagctca atgtgggcct
    19141 ggcggaagcc cagttcaagc tggccgagca ggatctcgtc ctgcgcgtcg ctcaggctta
    19201 tttcgacgtg ctggccgccc aggacaccct ggtggccgcc tcggccctgc gcgacgccaa
    19261 tgcccagcag ctggcgctcg ccaagaagag cttcgaggtc ggcaccgtga ccatcaccga
    19321 cgtgcacgag gcccagtccc gttacgatct gtcctcggcc cagaccatcg cggccgagag
    19381 cgacctggaa gtccgccgca acgcgctggc tgtgctcacc ggcaagacgc cggacgccct
    19441 caagaccctg cgcaagggcg tggccctgtc cagccccgag ccggcggaca tgccgtcctg
    19501 ggtgtccgcg tccgaagcca ccgccctgca ggtgcaggcg gcccagctcg gcgtcgagat
    19561 cgccgcgcgg gaaatcgagc gcaaccgtgc cggccacgcg cccaccgtcg atctggtggc
    19621 gagttacggc aactcggtca atccggtcgc ctcggtgacg cccaccacca actacgattc
    19681 ccgcgccatc ggcctgcagg tggccatccc cctgtaccag ggcgggggcg tcaattcccg
    19741 cgtacgtgag gccatcgccc tgcgcgacaa ggccgccgcg gatctggaca acgcccgtcg
    19801 cactgccgcg caggcggccc gtcaggccta tctgggcgtg accagcggtc tcgcccaggt
    19861 gcgcgccctg gaggccgccc aggtctcgtc ccgctccgca ctggacgcca acaagctggg
    19921 ctacgaggtc ggcgtgcgca tcaacattga cgtgctcaac gcccagagcc aggtcttcga
    19981 caccgagcag aagctctcca aggcgcgcta cgacacgctg atggcccagc tcaagctcaa
    20041 ggcggccgcc ggcaccctgg gggacgacga cgtccgcgcc ctcaacgccc tgctggactg
    20101 agccacgcgc cgggagcccg tgccgccgac ggccgctttg cggccgtttc catttgcggt
    20161 caatccgtga ccgccgtccg ggccgttccg tttatccttc cttcattccg acgttccgac
    20221 ttccgaaccg attcccatgc gttacaccga cctgcgtgac ttcatcgccc agctcgagcg
    20281 cctcggcgag ctcaaacgcg tccgcgtccc cgtcgatccc catctcgaga tgaccgagat
    20341 ctgcgaccgg gtgctgcgcg ccggcggccc ggcgatcctc ttcgaacagc ccacggggca
    20401 ctcggtgccg gtgctggcca acctcttcgg cacgccccag cgggtggccc tgggcatggg
    20461 cgagagcggc gcggactggc aatccgcttt gcgcgaggtg ggcaagctgc tggccttcct
    20521 caaggagccg gagccgcccc gcggcctcaa ggacgcctgg gacaagctgc cggtgttccg
    20581 ccaggtgctc aacatgtcgc ccaagacggt ctcgtcgggg ccctgccagg aggtggtccg
    20641 ggaaggccgc gacgtggatc tggccagcct gcccatccag cattgctggc cgggcgacgt
    20701 ggcgcccctg atcacctggg gcctggtcgt gacccgtggg ccccacaagc cgcgccagaa
    20761 cctggggatc taccgccagc aggtgctcgg ccccaacaag gtgatcatgc gctggctggc
    20821 ccatcggggc ggcgcactcg attttcgcga ccaccagcag gcccatccgg gcgagccctt
    20881 cccggtggcg gtggtgctgg gctgcgatcc cgccaccatc ctcggcgcgg tgacgccggt
    20941 gccggattcc ctgtcggaat accagttcgc cggcctgctg cggggcgcca agaccgagct
    21001 ggtgaaatgc ctggggtcgg acctgcaggt gccggcctcc gccgagatcg tcctcgaagg
    21061 cgtcatccat ccgggcgaga cggcgctgga aggcccctac ggcgatcaca ccggctatta
    21121 caacgagcag gccgagtttc cggtcttcac catcgagcgc atcacccacc ggcgcgaccc
    21181 gatctaccac agcacctaca ccggcaagcc gcccgacgag ccggccatgc tcgggctggc
    21241 cctcaacgag gtcttcgtgc cgctgctgca gaagcagttc ccggagatcg tggacttcta
    21301 cctgccgccg gaaggctgct cctaccgcct cgccgtggtg agcatccgca agcagtaccc
    21361 cggccacgcc aagcgggtga tgttcggcat ctggagcttc ctgcgccagt tcatgtacac
    21421 caagttcatc attgtcgtgg atgatgacgt gaacatccgt gactggaagg aagtgatctg
    21481 ggcgctgacc acgcgcatgg atgccacccg cgacaccacg ctggtggacc atacgcccat
    21541 cgactacctg gatttcgcca gcccggtggc gggtcttggg tcgaagatgg gcctggatgc
    21601 caccaacaag tggcccggtg aaacccagcg ggaatggggg cgcgccatcg tcatggatgc
    21661 ggccgtcaag gcccgtgtcg atgccctgtg ggcgcagctc ggcctgtagg tgtccttcgc
    21721 cggtcccttc ggcccggccc gctcttagtc tgactcgagg tgtccatgtt gtgccgtatg
    21781 ctcgcccgcg ccctgttgag cgcgggggtg atcctgatgc ccgcattcgc gtccgccgac
    21841 ctcgtcggcg ccggcgcagt ccagttcgaa accgaccgcc tggccgaagg gcaggctgcg
    21901 ctgcggagcc gccagctgga gcgggccgag cgcgccttca gtgaagcgct gcgggccaac
    21961 gccggttcca ccgacgccct gatcggcctc gcccagaccg cccagttgca ggggcggccc
    22021 gacaaggcgc gggactggat ggccaaggcc gtggcagcgg cgccgggttc accgccaatc
    22081 ctgcaggccc agtccaagct gctggtggag cagggcatgg gcgccctggc cgaggagcac
    22141 taccgtcgtg ccatcgccgc caatcccggc aatccccagt tcagccagga tctggcgagc
    22201 ctctatctgg agcagctcaa gcgtcccgcg gcggcggtca cggtgctccg cgacctcctc
    22261 cgtcgccagc ccgagctggc ctcggcctac ctcgggctgg gcctggccct ggccgcggac
    22321 ggcaagctcg acgaagccgg tcgcacgctt gacgaagccg tccagcgcga tccggtgaat
    22381 cccttcgcct accatgccca ggggctgctg gccctcaagc aggggcgcgc ggatgcagcg
    22441 ctgacggcct tcgacaaggc gctcgccctg cgccccgagt ttgtcggcgc gctcctcggc
    22501 aggggcgatg cgctcctggc cctgggcaag gccgatgggg cgctggcgac ctatcgccgt
    22561 gccgcgtcgc tggcgccggg cagtgcggtg ccgatcgcca agcgggccca ggcgctggag
    22621 cgcctgaagc gtccggatga agcggaagcg gcgtatcgcg aggcgctcaa gctggagccg
    22681 acccacacgg tcgtcctgaa caatctcgcc ttcctgctag cgtcccgaaa gcagcggctc
    22741 gacgaggcgc tcgggctggc ccagcgggcc acgacgctgg atccggcgcg ggccgatcac
    22801 ttcgatacgc tgggggtggt gctgctggcg cggggcgatc aggccggcgc gcgcaaggcc
    22861 ttcgagcggg ccgtggcggt cgatcccgcc cacgccggcg cacgtcagca cctggcccgt
    22921 gtcggcgcgc cggcggaggc gcccgcggcg gcggggcctt ccgcgcctgc cgcttcaccg
    22981 cgggagatcc cgaaggaggc cgcccggagc agcacggacg atgctgcccg ggccctctcc
    23041 acgcgccttg aggcgtggcg acaggcgtgg gaatccaagg atgccggacg ttacctgagt
    23101 ttctacggga aagatttcgc accgcccgac ggacgcgacc gcggcacctg ggaagccgag
    23161 cggcgggtcc gcctcgacaa gaagggcgag attcacgtgc agatcgttgc ccccgaactg
    23221 cgggtggagg gcgacacggc ggcggtcagc ttcgagcagc gctaccgggc cggtgcgctg
    23281 gtggacacgg gccgcaaacg catggcgtgg caccgggaag gcggggaatg gatgatccgc
    23341 cgggaaggca tgcagtagcg gcgcgcgtgt tcagacggcg ctggcgagcc gccaggcgtt
    23401 gccgccgcgc cgtttggcag catacatggc ggcgtcggcg gccgcttccc aggcatcggc
    23461 actggggtaa ggaccgggga tcaccgccgc gccgatgctg ccggtgacgc cgtaacggcg
    23521 cccgtcttcg gtggtgagcg cggcgatttc cgcgagcagc cgctcacaca gtgggccgat
    23581 gtcgttctcg gcggaaatgt ccaggcagat cagcgcgaat tcatccccac ccaggcgtcc
    23641 caccagatcg gtattgcgcg caacctgccg caggcgttcg gacacggccg ccagcagcag
    23701 atcgcccacg gcgtgcccga aagtgtcgtt gatcggcttg aagccgtcca ggtcgatcag
    23761 cagcagggtc gcgctgcgct gccagcgcac ggcggcgtcg tgggtgctgt cgcgggcttc
    23821 ccggtaggcg gtccggctgg ccaggccggt catcgggtcg aattcggcaa ggcgccgcca
    23881 gcgctggctt tcctgggtca gattgcgata gcgccgtacc aggccgacga cgatcaccgt
    23941 gaagttcccg agggccagga cgaaggcgcc aatctggatg ctgcgcaggg tgcgggtgcg
    24001 ttgcacggag gcctgttcca gtgccgaggt gaggcggttc atttcagcca ggatgtcccg
    24061 gttgtgctgc accatgtagt gcgtggcttc acccagttcg gaatctgcca ggggcttgcc
    24121 ggcgaggaat tcgggcaggc gcgcgtccag cggcgccatc agggtcgcca cccgttcgac
    24181 ggcatcgagt ccggcgcccg ggtcgatcgg ccgcaggtgc acggacgggc cggtgccgcc
    24241 ggccgtgtcg ccgccatcgt ggaaggcacg gagggtccgt tcaaagaggc gataggcggt
    24301 acgcagttcc tgttctgcat cgcgacgggc atcggccgag gggacgtgga ccgccaggag
    24361 cagcgccttg gtgatctcct gggacagcat gcgctgacgg ccggccaggt tgatggccac
    24421 cgcatcttcc gccacctggg cggcgatcca gacattgatc agcagcacgg ccagatcgag
    24481 caccacgaac aggacgatgg cactgaggag gcggcgcggc gaggcgatga gctggtcgat
    24541 gcgaataaac atgggagtcc gcacggcgga gagggacgtc tcatgtcggt caacgaccgc
    24601 gaatttggtg gctttagtaa atgacgctgg aaaattgtca tgaaaaaggg cggctccaga
    24661 gagccgccct attggattcc cgccggggag cgatcaatcc ttgaagcgtt cttccagggc
    24721 cgcgatggcg ggcagggtct tgccttcgag gaattcgagg aaggcaccgc cacccgtgga
    24781 aatgtagccc acgtcgtcgg cgatgtggaa cttggcgatg gccgccaggg tgtcgccgcc
    24841 gcccgcgatg gaaaaggctt cggagtgggc gatggcggag gccatcatct tggtgccgcc
    24901 ggcgaactgg ggcagctcga agacgcctac cgggccgttc cagacgatgg tgcccgcgtt
    24961 ggcgatgatc tgggagagct gcgcagcggc cttgggaccg atgtcgagaa tgcggtcgtg
    25021 ggggccgacc tcatccacgg gcactttgtt ggcgcgggcc atggcagaga cttcgtcggc
    25081 cacgaccacg tcttcgggca ggggcacttc ggcgccacgg gccttcatca ggtcgatgat
    25141 gacctgggct tccttgacca gctcgggctc ggcgagggat tcgccgatgc gcttgcccga
    25201 ggccagcagg aaggtgttgg cgatgccgcc gccgacgatg agctggtcca ccttgtccgc
    25261 cagggacttg aggatggtca gcttggagga caccttggag ccgccgacga tggccaccag
    25321 cggacgcttg gggctgtgca gggccttgcc cagggcgtcg atttcggcgc ccatcaggat
    25381 gccggcgcag gccgtggggg caaagcgggc gatgccgtgg gtggtggctt cggcccggtg
    25441 ggcggtgccg aaggcgtcgt tcacatagac gtcgcagagg gcggccatct tgcgggccag
    25501 ttcctcgttg tccttctttt cgcccttgtt gcagcggcag ttttccagca gcacgacttc
    25561 gcccggggcg acgtcgaaac caccgtccac ccagttctgg atcaggcgca cgggcttgcc
    25621 caggagctgg cccagacgca cggcgacggg cgccagggtg tcctcggcct tgagttcgcc
    25681 ttcggtggga cgtcccaggt gggacgtgac catgaccgcg gcgccgcctt cgacggcgta
    25741 gcggatggac gggatggagg cgcggatgcg ggtgtcttcg gtgatgttgc cggcgtcgtc
    25801 ctggggtacg ttgaggtcgg cacggatgaa gaccttcttg cccttgacgt cgagatcggt
    25861 gagtttcttg acttgcatga tgtggttcta cggagcgtcg gaatgaaaaa cgccccgcgg
    25921 agcggggcgt cggtgaggcg gatgatcaga cgttcatcca ggcgcgggcg gcgtccagca
    25981 tgcggcagga gtagccccac tcgttgtcgt accaggccag caccttgacc a
//
LOCUS       DAOQMM010000045        25178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_613_length_25178_cov_44.565538, whole genome shotgun sequence.
ACCESSION   DAOQMM010000045 DAOQMM010000000
VERSION     DAOQMM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 25178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25178
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_25178_cov_44.565538"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="PLH85_09150"
     CDS             complement(<1..1705)
                     /locus_tag="PLH85_09150"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNB64769.1"
                     /translation="MKISFRRSACTIAAAILLPVTHAQGSPDLSGLLSILNSTPEGTW
                     VQVNLNSYSEAWTPAELRPLYLTSNPDPSKITYAWPSFAWDSNRGDLILYGGGHANYS
                     GNDVYRWHGTTRKWERAALPSELLLNPPGFYTPIDGPDAGPPSSHTYDNSLFLPIIDR
                     FIVFGGAAFNTGGPYNRMNPDGVTYRNTGPYLFDPSKADPNKVGGTTGSHVKRVAPHP
                     EIVGGNMWTNRDYRAYTFTGSTLIPNSHINGVTAYAQENGKDVVYFGARPGQGTDQHL
                     FKYTISSLTDVTQDKLEKVGRFWNGPSDQGAGTYDPTRKLFVRTGPATQPITYWDLNN
                     ASPSNNDVVTVPVDASGTFDFNQMRMTGIDYDPTTDQYLIWYGGPDVWRLTPPTPVTS
                     TGWKLDKVSKTSSAAPTIDADSRGILGKWKFIPNLNAFLGLQDFVNGNIWLFKPVGWK
                     LPTGTGNLPPTVSLTSPSAGASFAVGSIVSVTATASDSDGSVTQVAFYVDGGIATTSS
                     GPTYTANLSGLTAGTHSLTAVATDNAGAITTSAAVSITVTSATNTPPTVSLTSPAAGA
                     SF"
     gene            2001..3281
                     /locus_tag="PLH85_09155"
     CDS             2001..3281
                     /locus_tag="PLH85_09155"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HNB64770.1"
                     /translation="MSPTPSDVSPAASANQPVSPPRRRLWPWFLVLALALGGVYAWRT
                     SMSESKVHAEDRGRNRGDRPQPVLLGEVQAGDIRVYVSALGSLVPRNQVTVRARVDGQ
                     LMKVVFREGQAVKAGDLLAEVDARPFEVAVAQAEGQLLRDKALLQNARIDLDRYKGLL
                     AQDSIARQQVDTQEALVRQYEGTVKADQGAVDSARLQLSYTRITAPIAGRAGLRQVDP
                     GNIVHASDTTGVVTLAQVSPITAVFAVPEERVAEVMARQKAGKPLAVEAWDREQRTLL
                     ATGRLIAADSQIDTTTGTLKLKAEFANEDGSLFPNQFVNVRLILDRLKAVPVLPTAAL
                     MQGDKGRYVYRVGADNTVAAVTVRLGAVDGERIAVEGGLDLGDKVVVDGLDKLRDGAA
                     VEAIAPDAANAPGRKRPPKGGPGGGGPGGGRPAH"
     gene            3294..6380
                     /locus_tag="PLH85_09160"
     CDS             3294..6380
                     /locus_tag="PLH85_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HNB64771.1"
                     /translation="MNPSRLFIVRPVATALLMVAILLTGLVAYRLLPIAALPQVDYPT
                     IQVKTFYPGASPDVMTSAITAPLERQFGQMPGLDEMSSTSSGGASVITLRFSLDLTLD
                     VAEQQVQAAINAASNLLPTDLPAPPTYSKVNPADTPILSLAISSPSLPLTRVHDLVES
                     RLAQKIAQVPGVGLVSIAGGRRPAVRVQVNPTQLAAYGLALEDVRTAIANANITQAKG
                     SFDGPQRASTIDANDQLRSADDYRKLIVTYRNGSPVRLDDVADLKDDAENLRLAAWAD
                     TTGAVIVNVQRQPGANVIEVADRVRALIPQMTASLPASLDVRVLSDRTTTIRASVHDV
                     QVELALAVGLVVMVIFLFLRSVPATLIPSVAVPLSLVGTFGVMYLAGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYVEEGDSPMEAALKGSRQIGFTIISLTLSLIAVLIPLL
                     FMADVVGRLFREFAVTLAIAILISAFVSLTLTPMMCARLLRHVPEAQQGRFARAAGDF
                     IDRVIARYGRMLAWVLDRQGATLLVALATLGLTAALYILVPKGFFPVQDTGAIQGITE
                     APQTISFAAMAERQQAVAAVILRDPAVASLSSFIGVDGTNATLNSGRLLISLKPLAER
                     GVTATEVIRRLAPELARVAGITLYMQPVQDLSIEDRVSRTQYQFSLQSPDAAALAEWS
                     PRLVERLRALPQLADVASDLQDKGLQAYVDIDRDAAGRLGVTTAAIDNVLYNAFGQRL
                     VSTIFTQASQYRVVLEVLPEFRTRPEALAKLYVPTLAGVQVPLSAVARVSERPTTLAV
                     NHIGQFPAVTLSFNLAPGVALGDAVEAIRRAEAELGLPASIETGFEGAALAFQSSLSS
                     TLLLILAAVVTMYIVLGVLYESTIHPVTILSTLPSAAVGALLALLLARAELDITAVIG
                     IVLLIGIVKKNAIMMIDFALEAERHQGMRPREAIYQACLLRFRPILMTTLAALLGALP
                     LMLGTGVGSELRHPLGITLVGGLMVSQVLTLFTTPVIYLAFDRLSLRLQRRTATAP"
     gene            6377..9487
                     /locus_tag="PLH85_09165"
     CDS             6377..9487
                     /locus_tag="PLH85_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNB64772.1"
                     /translation="MNLSRLFIFRPVATSLLTLAIALAGAIAFRLLPVSPLPQVEYPT
                     ISVSASLPGASPETMAATVAGPLERILGQIAGVNEMTSTSTLGSSRVTLQFDLSRDID
                     GAARDVQAAINAARGQLPTSLPNNPTYRKVNPSETPILILALTSTTLTQGQLYDVAST
                     VLAQRLAQVDGIGQVSIGGSSLPAVRVEANPDRLAAAGIALDDVRTAIVGAVANRPKG
                     VLDDGQRAWQIGANDQAYTAREYRPLIVTWKNGAAVRLGDVARVRDSVQDERNFGSAN
                     GRPAVLLILNRQPGANIIATVDRVTALLPHLRALVPAAVDLDVVMDRTPTIRGSLREV
                     ERTLVISVGLVVMVVFLFLRNVRATVIPSIVVPVSLIGTFAIMYLAGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENINRHVEDGHTPLRAALRGGREIGFTVISMSLSLIAVFIPIL
                     AMGGIVGRLFREFAVTLSAAILVSMVVSLTATPMMCARLLRPTPPAAGRGRFDRWIGG
                     AFERLLAGYRHSLGWALDHPRLILAVLLLTVGCNVYLYNAIPKGFFPQQDTGRIRGFV
                     EADQAISFQAMRGKMERLLALVGADPAVDNVVGFTGGFQLNYGSMFVTLKPLGVRTES
                     ADAVVARLRRQLAHFPGANLYLNPVQDIRIGGRSSNSTYQFTLQADRLDDLRLWEPRI
                     RNAMSRLDELTDVNTDQQDRGPQTSLVVDRDAMTRYGLTMRSLTTALNNAFGQRQVST
                     IYNPLNQYRVVLEAAPAYLQDPDVLSRLTLVASGGQPVPLSAVARFESTLAPLSVNHQ
                     DGAVASTISFNLPEGKTLGEATRAIDAALQQIGVPVSIRTSFQGSAGAFQQSMSTQPL
                     LILAALISIYIVLGMLYESLVHPLTILSTLPSAGVGALLALLAVNMEFSLIAMIGVIL
                     LIGIVKKNAIMMIDVALALERSEGVGARAAIHHACLLRFRPILMTTLAAICGAVPLAI
                     GTGDGAELRQPLGIAIVGGLVLSQILTLYTTPVVFVLLDRLRSRAGAARAPVPDLARS
                     PAGNLS"
     gene            9484..10911
                     /locus_tag="PLH85_09170"
     CDS             9484..10911
                     /locus_tag="PLH85_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNB64773.1"
                     /translation="MKTCLRPLPVILVVLLSACRIGPDYVRPEMAVPTVYKEAGDWKP
                     AAPADPAAGDAWWTAFGDPVLDQLQARLLVSNQNLRAVEAQYRQARALADSARASGFP
                     TVTGSVGMTRGTSGSSGSSTASQGPAAVTNSYSLGASSSWEADVWGRIARNVEAADAR
                     VEASDADLAAARLSARATLAQTYFQLRSAERQKRLLEATVAAYERALELTRNRYAAGV
                     AGRADVVQAESLLRSTRAQTIDVRLSRAQYEHAIAVLIGEAPAAFTLAESDAPLPVAV
                     TPVSLPSTLLERRPDIAAAERRVAAANAAVGAAQAARFPVLGLTAGAGYRSSDLSELL
                     SLSSRYWSLGPTVAASLFDGGAKRAATGQAVAVWDQAVATYRQTVLTAFQEVEDNLVA
                     ARLLEHELTEQNAAVTAAAEAEAIALNQYRAGTVSYLNVVTAQTTLLTARRSANDLAA
                     RRLLAAVLLAKNAGGDVARSAKPAP"
     gene            complement(10972..12186)
                     /locus_tag="PLH85_09175"
     CDS             complement(10972..12186)
                     /locus_tag="PLH85_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64774.1"
                     /translation="MMRLNSTTLNVLIASLLCGGCASTSVSDSLWTQVSGDGNRIVLR
                     LDPEHPRLRQFASAQINLAADFVDDSGTPRTASLGGGSRVDAQTRVYTLPETLNNPPS
                     GEICLYFSPADTRLGAFPIRARTAGGTDNSGFRYPDWEARVGAETRRRLNDRQKSGLD
                     AQRNAAQRSVQQLQAELDKDQLASVEDCARRTFTAKADARGRDVIEPAAQAATARRIC
                     VRRTRELNLTSKFSVVDALGQFRNQLPSERSRQAGQFLTDWRNHFERTGADYVPELGS
                     PREPLAIATDAETLAARPSLQPRDSIALIGAWLDAYDVCVQDTTHQLGHRYQLWQVSQ
                     GRSSDRAAAYSDYMAQECRGKFRRLNEQRALLADVDRQLGALPSAAPTSSPQAQNRNV
                     SLNALACTLPPR"
     gene            complement(12183..13079)
                     /locus_tag="PLH85_09180"
     CDS             complement(12183..13079)
                     /locus_tag="PLH85_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64775.1"
                     /translation="MNARFLAGARRLCGLAVLLAVAPLPAVALDFTPVTLDNGLKLLI
                     VHDKRPCARNESCGFTAGDANRLKDFLERDRYVQVLLNSNGGSSTEGQRVGAVLRARG
                     AWVRVAKGHHCISACTAAFLGGVIREVDEGGEFHVHSSSSYLNGVSEEEWQRVSRDPD
                     LELGNTLRATVADRVEWTLSRFAYVQRMIAGESPKGLLEGSNSECLSRAQDQLLQPGR
                     LRQLAQRVRVEGRPALNEIEEATERRTTELCVGILTAQADRLGPRAPQVLRIMDIMTA
                     SRIGATINLSPQALREAGFVTQ"
     gene            complement(13084..13455)
                     /locus_tag="PLH85_09185"
     CDS             complement(13084..13455)
                     /locus_tag="PLH85_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64776.1"
                     /translation="MAGKEVSKRQSKPNKNTAITLALLGCVGADALAGTVYAKLSENG
                     RRAREIALLEAARSGRVQTYRDDQAPTLTGTVTPGPRNRDAAGSLCVTNTDYLADRGK
                     GENVYVKMCQQADGGWAPAVS"
     gene            complement(13947..14282)
                     /locus_tag="PLH85_09190"
     CDS             complement(13947..14282)
                     /locus_tag="PLH85_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB64777.1"
                     /translation="MTPPPDDLSPCQACGACCAQFRVSFYWSEALARTLPDAYVEQIN
                     PWMSCMAGTQGPAPRCAALHGAVGTDVRCTVYPHRPDACREVQAGDDKCERARACHGL
                     PPLGPSAPS"
     gene            14548..15366
                     /locus_tag="PLH85_09195"
     CDS             14548..15366
                     /locus_tag="PLH85_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64778.1"
                     /translation="MNTFFSRLMTLGVVTLAGLGVAGPAAANPFQIATDGSGLAGAQL
                     LSSFDLGGHGFVFQYMDPTSGLPMFQENGAFRIVASDGVSPIGTNDLTLTYALWGTAD
                     PLSGLISLIGGGFDLYSDSSFDFGTSTGIYGADDGTHVASFLVTGGSGDPMGTHLVSM
                     SAQLVAGSLLPGYLFTADHRDMSQLASVTLNASFVDNVATPDATAVEELVCEQAGLAA
                     LGCQGTAYANQIPYYFLVEDSASVTLNAVPVPGSLCLGALGLAMLGMHRRRMPG"
     gene            complement(15462..16691)
                     /locus_tag="PLH85_09200"
     CDS             complement(15462..16691)
                     /locus_tag="PLH85_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB64779.1"
                     /translation="MESHSIEVAKHVLVTFGIILAIGSVSGFASRKLRMPDVAVFLLV
                     GMAIGPSALGWVDVKADSALNQIILIFGSSYILFDGGASLRLAVLKNVWVSIVLLSTL
                     GVLITAFITGYAAQWILGIPFIVALLLASAIASTDPATLVPVFRQVQIRERVAQTVMS
                     ESAFNDAVGAILTFAVLGLAMGQGEFSLSASLADLAVQAGVGLVAGLVLGWLAALFIA
                     HEKYAFLEEYAPVVSLMAVIGAYLGADGLHASGFMAVFVFGMVIGNKDVLGFEMGEEE
                     QHKLEEFVAITALIMRMFIFILLGSQVDFALMRAHLWEGLAVVAVFMFVARPATVFLC
                     ALPDRKARWSWQEMLFMCWTRETGVIPGALAGLLMGMGAPEAPLIASVTFIAILVTIL
                     LQATTTKWLAGRLGLLE"
     gene            complement(16895..19234)
                     /locus_tag="PLH85_09205"
     CDS             complement(16895..19234)
                     /locus_tag="PLH85_09205"
                     /inference="COORDINATES: protein motif:HMM:NF018620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HNB64780.1"
                     /translation="MRPLFALLIVFVTGCTAVAAHRLDERLGPRDPGRYDQAPAPVAP
                     APDYWTQVRPILDRRCVTCHACYDAPCQLNLSRYDGITRGASAETVYSSTRLTAAQPT
                     RLGFDALSTRAWRERGFFPVLNERSPTPEANREGSLLLRLLALKQAQPGPGSGALPDK
                     DFDFSLDRSQACAPLEGVGAYERSHPLWGMPFGLPALRSAEHDILSRWIEAGAPYTPP
                     PPAPPAVARRVAEWEALLNADAPGAQLAARYIYEHWYIGHLYFDEAPDIRFELVRSRT
                     APGAPIDPIATRRPYDDPGVARVYYRLRPNEATPVAKTYMPLVLDSARLTRLRKWFFD
                     TPATVDRLPGYAPETAANPFATFRALPVDSRYRFMLDDAKFTMMGFMKGPVCRGQVAL
                     NVIDDHFWVLFYAPEGEVSRNVQGLLDATQPNLRMPAEDSSSVGALAWLAYARAERNY
                     LNTKSAFMAGKTAVRPRLEDLWTGDGRNPNAALTVFRHFDSASVVRGLAGERPQTVLL
                     LGYPLFERMHYLLVAGFDVYGNVGHQLATRLYMDFLRMEGEQNFLTLLPLKDRQKVLD
                     HWYRGRSEPRIRQFADTSAYYPGETGVRFHGPDSLGELYRLAQRHVRPVREAGLDFAG
                     TGFSPAQGALLARLSAVKGRSASWMPELSLLALRKADGSVQPVTLIRNSAHSNVAQMF
                     NEDARRLPEEDTLLALPGFAGAYPNAIFQLDPAELPRFVNAVEGLTGEDDLLRLVQRF
                     GIRRTDSRFWPVSDALHDAWQRSSPREASILDYSRLENR"
     gene            complement(19328..19741)
                     /locus_tag="PLH85_09210"
     CDS             complement(19328..19741)
                     /locus_tag="PLH85_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNB64781.1"
                     /translation="MTSSLYGPMLALIAWTGGVGMLAARRRVREIRRRRIPLATLARP
                     RDLAQALDDTQAMDNFNNLLQVPVLFHLCCLAFAQQASVPQAAAILAWVYVALRIGHS
                     LIQLTHNRVLPRFGVWTAGNLVLMGLWGVFAAGLV"
     gene            complement(19775..20338)
                     /locus_tag="PLH85_09215"
     CDS             complement(19775..20338)
                     /locus_tag="PLH85_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNB64782.1"
                     /translation="MTRFVPFATSLALLAFATATPVLAEDFTVSSPSFSAAAPLPERH
                     VLDSFGCHGQNLSPALRWDHPPAGTRSFAVTLYDPDAPTGSGWWHWVVVDISADQRAL
                     PEGAGNAGRPGLPAGALQGRTDYGAPGYGGACPPEGAPPHRYVLTVHALKVERLGVPP
                     DASPAMMGYMIHMNRLGSAVVQTTYGR"
     gene            complement(20483..21292)
                     /locus_tag="PLH85_09220"
     CDS             complement(20483..21292)
                     /locus_tag="PLH85_09220"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB64783.1"
                     /translation="MDAAHGARVVATQAYTLKTLTIRDDAFIAVLGGRKTLHAPHARL
                     SADVQQGVMLARGTQWDVTNDPAGRREYAALAIAFPAELIRECHALEAAAGLPEVDAA
                     QVLAVDEELLAAVQRTLPPVAARPLAGALLRHRVLEVLILLAQRGHRFAPAESLPWPE
                     RVRRLVAQRLHADWDVATLATAFHVSESTLRRRLEGSGTTLAALVRDIRLETALGLLQ
                     TTRLPVGEVAARCGWKSHSRFTVAFQERWGVPPSVVRARLKDDAQDLTETA"
     gene            complement(21415..21810)
                     /locus_tag="PLH85_09225"
     CDS             complement(21415..21810)
                     /locus_tag="PLH85_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB64784.1"
                     /translation="MPTNPVVHFEIYVQDMARARAFYENVLGTTLVRMPAPTPEMDLD
                     MLFFPMDKDTGMNTYGSGGMLVRMEGMASGGGGTLVYFACEDCAVQAARAAEFGGRLC
                     KEKTSIGEHGFFSLAQDSEGNMIGFHSMK"
     gene            complement(22053..22715)
                     /locus_tag="PLH85_09230"
     CDS             complement(22053..22715)
                     /locus_tag="PLH85_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64785.1"
                     /translation="MADNNRSWIRDLLAVPLVVGIVIAIFTYALPKLLTESSQLSYTI
                     EEPVAYLDKTSIGTASVKVNDISVPEVFAVRVRIWNSGSLPLKNLGVLFEFAATDKDF
                     RVLSVNHNTNPPKEFGAVTEEGNDTNSKRFIYALLNSEDADSLVFLTTAKADVKVFSK
                     AENLSVKPVSQEKRTGFHWYDAAILAMLVSLVSSFIEFLLKVWRERRRNKKSTQAGES
                     GI"
     gene            complement(22799..23461)
                     /locus_tag="PLH85_09235"
     CDS             complement(22799..23461)
                     /locus_tag="PLH85_09235"
                     /inference="COORDINATES: protein motif:HMM:NF037410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64786.1"
                     /translation="MSRLFTYTITVDDGAAPNPFHGMCSLAICKPGIRRVAKPHDWVA
                     GLGSKNAPSGDLSGHLIYAMRVEQVISLRDYDINARTQWPHRIPNIQSSALQDRLGDC
                     IYDFSSGSAVQRAGVHGLGNVATDLSGENVLISKDFYYFGSRARRLPDYLLPICHQMQ
                     GHRSNSNAPFFDQFVSWVRSLVSTPGQLYGWPDHVVEWGSVSACGGCIPRKLDDEFDP
                     DC"
     gene            complement(23477..24550)
                     /locus_tag="PLH85_09240"
     CDS             complement(23477..24550)
                     /locus_tag="PLH85_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB64787.1"
                     /translation="MDQQPQQITPHEDSQRHRKALVFISHDTRDAEIAEAFSRLLSSV
                     SAGVLKSFRSSDKKGNQGIEYGVEWYPEIMKKLETASDVVCLLTPNSVNRPWLLYEAG
                     VAKGKLDTPVHGVALGIPLHVASTGPFAQFQNCDDAEDSLTSLVIQLVGRIPNAEPDR
                     DVVKIQVEAFKQKISPLFEAKSAEIRTKEEADEASIAKIFEEIKVMFQDLPGRIERRI
                     DPESSAEIRRRRKIHPHFLDKIMHIAGKETGDPVVLLIVCSLFKDDFPWLYEIALDVY
                     RKFESRSVDAPDALKRFVRLFEIATHHPMFREFGPRSRERRFELEETMSFALHTMERT
                     LSAIGANPPLNTDAPPSGGASVS"
BASE COUNT         4103 a         8231 c         8489 g         4355 t
ORIGIN      
        1 tgaagctggc accggcggcg gggctggtga ggctcacggt gggcggcgtg ttggtcgcgc
       61 tcgtgacggt gatgctcacg gccgccgagg tggtgatggc gccggcattg tcggtggcca
      121 cggcggtgag gctgtgggtg ccggcggtga ggccactgag gttggcggtg taggtggggc
      181 cggaggacgt ggtggcgatg ccgccatcga cgtagaaggc gacctgggtc acggagccgt
      241 cggagtcgga tgccgtggcg gtgacggaaa cgatgctgcc gacggcaaag ctggcgccgg
      301 cgctcgggct ggtgaggctg acggtgggcg gcaggttgcc ggtaccggtg gggagcttcc
      361 agccgaccgg tttgaacagc cagatgttgc cgttgacgaa atcctgcagg cccaggaagg
      421 cattcaggtt gggaatgaac ttccatttgc cgaggatgcc gcggctgtcc gcatcgatcg
      481 tcggcgcggc gctgctggtc ttgctgacct tgtcgagctt ccagccggtg gaggtcacgg
      541 gcgtcggcgg ggtcaggcgc cagacatcgg ggccgccgta ccagatcaga tactggtcgg
      601 tggtcgggtc gtagtcgatg cccgtcatgc gcatctggtt gaagtcaaag gtgccactgg
      661 catcgaccgg gacggtgacc acgtcgttgt tggacgggct ggcgttgttg aggtcccaat
      721 aggtgatcgg ctgggtggcc ggccccgtgc ggacgaagag cttgcgggtc ggatcgtagg
      781 tgcctgcgcc ctggtccgat gggccattcc agaagcggcc gaccttttcg agcttgtcct
      841 gggtgacgtc ggtcaggctg gagatggtgt atttgaacag atgctggtcg gtgccctgtc
      901 cggggcgggc gccgaagtag acgacgtcct taccgttttc ctgcgcatat gccgtgacgc
      961 cgttgatgtg gctgttggga atcagggtgc tgccggtgaa ggtgtaggcc cggtaatccc
     1021 ggttggtcca catgttgccg ccgacgattt ccgggtgcgg ggcgacgcgc ttgacgtggg
     1081 agccggtggt gccgccgact ttgttcggat cggccttgga ggggtcgaac aggtaggggc
     1141 cggtgttgcg gtaggtgacg ccatcgggat tcatccggtt gtaggggccg ccggtgttga
     1201 aggcggcgcc gccgaagacg atgaagcggt cgatgatggg caggaacagg ctgttgtcgt
     1261 aggtgtgcga cgaaggcggt ccggcgtcag gcccgtcaat gggcgtgtag aagccgggag
     1321 ggttcaacag gagttcactg ggcagagcgg cgcgttccca cttgcgggtg gtgccatgcc
     1381 agcggtagac gtcgttgccg ctgtagttgg cgtgcccgcc gccatacaga atgaggtcgc
     1441 cccggttcga atcccacgcg aaggacggcc acgcgtacgt gatcttcgac gggtcggggt
     1501 tggatgtcag ataaagcgga cgcaattcag cgggcgtcca ggcttcactg tagctgttca
     1561 ggttgacctg gacccaggtt ccttccggcg tcgagttgag gatcgacagc agcccgctga
     1621 ggtcgggcga accctgggcg tgggtgacgg gcaggaggat cgccgctgcg atggtgcatg
     1681 cggaccgacg gaaggagatt ttcatgggcc aacctgtgaa acggtgtgtt gcagggcagt
     1741 aaatgacaaa tgcattgcat tttgcgaacg tagagcaatg atatttaaca ttctataccg
     1801 tgcgaatgca cggggctgcg actttcttca cggacgggtc gaacctgtcg gagcgtccat
     1861 cggggagcgc gctccgacag gttcgacccg aaccctcgca acgtcccgta gtctgatgga
     1921 agtcactcat cgccatcgtg taggattgtg cattcgccgg gactgcgctc gtccggcgcg
     1981 ccgaaatccg gaaaatcttc atgtcgccca cgccttccga cgtttcccca gccgcttccg
     2041 caaaccaacc cgtctctccg ccccgccgcc gcctgtggcc ctggttcctg gtgctcgccc
     2101 tcgcgctcgg cggcgtgtat gcatggcgca cctcgatgtc ggaaagcaag gtccatgccg
     2161 aagatcgagg acgtaaccgg ggtgatcgcc cgcagccggt gcttctcggt gaggtacagg
     2221 cgggggacat ccgcgtttat gtctcggccc tgggcagtct ggtgccgcgc aatcaggtga
     2281 cggtgcgcgc ccgggtggac ggtcagctga tgaaggtggt gttccgggaa ggacaggccg
     2341 tgaaggcggg cgatctgctg gcggaggtgg acgcgcgccc ctttgaggtg gccgtggccc
     2401 aggctgaggg acagctgctg cgggacaagg ccctgctcca gaacgcgcgg atcgacctgg
     2461 accgctacaa ggggctgctc gcccaggatt ccatcgcccg ccagcaggta gacacccagg
     2521 aggcgctggt gcgtcagtac gaaggcacgg tcaaggccga tcagggggct gtggacagcg
     2581 cccgcctgca gctgtcctat acccgcatca cggcgcccat cgccgggcgg gccgggctgc
     2641 gccaggtgga tccgggcaac atcgtgcatg cgtcggatac gaccggcgtg gttacgctgg
     2701 cccaggtgag ccccatcacc gccgtcttcg cggtgcccga ggagcgcgtc gccgaagtga
     2761 tggcccgcca gaaggccggc aagccgctgg cggtcgaggc gtgggaccgg gagcagcgca
     2821 ccctgctcgc caccggccgg ctcattgccg ccgacagcca gatcgacacc accaccggca
     2881 ccctcaagct caaggcggag ttcgccaatg aggatggcag cctgtttccc aaccagttcg
     2941 tcaacgtgcg cctgatcctg gaccgcctca aggcggtgcc ggtgctgccc accgccgccc
     3001 tgatgcaggg cgacaaaggg cgctatgtct atcgggtggg tgcggacaac acggtggcgg
     3061 cggtcaccgt caggctgggc gctgtcgacg gtgaacggat tgccgtggag ggcggtctgg
     3121 acctgggcga caaggtggtg gtggatggcc tcgacaagct gcgtgacggc gccgccgtcg
     3181 aagccattgc acccgacgcc gccaatgcgc cagggcgcaa gcgccctccg aaaggcggtc
     3241 ccggcggggg tggccccgga ggcggccggc cggcgcactg acccgccatg gcgatgaatc
     3301 cgtcccgtct gttcatcgtc cgcccggtgg ccaccgcgct gctgatggtg gcgatcctcc
     3361 tcaccggcct ggtggcctac cgcctgctgc ccatcgcggc cctgccccag gtggattacc
     3421 cgaccatcca ggtcaagacc ttctatcccg gcgccagccc ggacgtgatg acctccgcca
     3481 tcaccgcgcc gctggagcgc cagttcgggc agatgccggg gctcgacgaa atgtcctcca
     3541 ccagttccgg cggcgcctcg gtgatcaccc tgcgtttttc cctggacctg accctcgacg
     3601 tggctgagca gcaggtccag gcagccatca acgccgcctc caacctgctc cccaccgacc
     3661 tgccggcgcc gcccacctac agcaaggtga acccggccga cacgccgatc ctgagccttg
     3721 ccatctcctc gcccagtctg cccctgacgc gggttcacga ccttgtggag agccgcctcg
     3781 cccagaagat tgcccaggtg cccggcgtcg gtctggtgag catcgccggc gggcggcgtc
     3841 cggccgtgcg ggtgcaggtc aaccccaccc agctggcggc ctacggcctg gccttggagg
     3901 acgtgcgtac cgccatcgcc aacgccaaca tcacccaggc caagggcagc ttcgacggcc
     3961 cgcagcgggc ctccaccatc gacgccaacg accagctgcg ctccgccgac gactaccgca
     4021 agctcatcgt cacctaccgg aacggcagcc cggtgcgcct cgacgacgtg gccgacctca
     4081 aggacgacgc ggagaacctg cgccttgccg cgtgggccga caccaccggt gcggtcatcg
     4141 tcaacgtgca gcgccagccc ggcgccaacg tgatcgaggt ggccgaccgg gtgcgggcgc
     4201 tgatccccca gatgaccgcc agcctgcccg cgtccctcga cgtgcgcgtg ctctcggatc
     4261 gcaccaccac catccgtgcc tcggtgcacg acgtgcaggt ggagctggcc ctggcagtgg
     4321 gcctggtggt gatggtgatc ttcctgtttc tgcgcagcgt gccggccacg ctgatcccca
     4381 gcgtcgccgt gcccctgtcc ctggtgggca ccttcggggt catgtatctg gcgggcttca
     4441 gcctgaacaa cctcacgctg atggccctca ccatctccac cggctttgtg gtggacgacg
     4501 ccatcgtgat gatcgagaac atcgcccgtt acgtggagga gggcgattcg cccatggagg
     4561 cggccctcaa gggctcgcga cagatcggtt tcaccatcat ctccctgacc ctgtcgctga
     4621 ttgccgtgct gattccgctg ctcttcatgg ccgacgtggt cggacgtctg ttccgggaat
     4681 tcgcggtgac cctggccatc gccatcctga tctccgcctt cgtgtccctg accctcacgc
     4741 ccatgatgtg cgcccgcctg ctgcggcacg tgcccgaggc gcagcagggg cgcttcgccc
     4801 gcgccgcggg cgatttcatc gaccgggtca tcgcccgcta cggccgcatg ctggcctggg
     4861 tgctggaccg tcagggggcg accctgctgg tggcgctggc cacgctgggt ctcaccgccg
     4921 cgctctacat tctggtgccc aagggcttct tcccggtgca ggacaccggc gccattcagg
     4981 gcatcaccga ggccccccag accatctcct tcgccgccat ggccgagcgt cagcaggcgg
     5041 tggcggcggt catcctccgg gatccggcgg tggccagcct gtcgtccttc atcggcgtgg
     5101 acggtaccaa tgccaccctc aacagcgggc gcctgctgat cagcctcaag cccctggcag
     5161 agcggggcgt cacggccacc gaggtgatcc gccggctcgc gccggagctt gcccgggtgg
     5221 ccggcatcac gctctacatg cagccagtgc aggacctgtc catcgaggac cgggtcagcc
     5281 gcacccagta ccagttctcg ctccagtcgc cggatgcagc cgcgctggcg gaatggagcc
     5341 cgcgcctggt ggagcgtctg cgcgccttgc cacagcttgc tgacgtggcc tccgatctgc
     5401 aggacaaggg cctccaggcc tacgtggaca tcgaccggga cgcggccggg cggctcggcg
     5461 tgaccaccgc ggccatcgac aacgtgctct acaacgcctt cggccagcgc ctcgtgtcca
     5521 ccatcttcac ccaggccagc cagtatcggg tggtgctgga agtcctgccc gagttccgta
     5581 cccggccgga agccctcgcc aagctctatg tgcccaccct ggcgggcgtc caggtgcccc
     5641 tcagcgccgt cgcccgcgtc tcggagcggc cgacgaccct ggcggtgaat cacatcggcc
     5701 aattccccgc cgtgaccctg tctttcaacc tggcgcccgg cgtggccctg ggcgatgccg
     5761 tcgaggcgat tcgccgggcc gaagccgaac tgggcctgcc agccagcatc gagaccggtt
     5821 ttgaaggcgc cgccctggcc ttccagtcct ccctgtccag caccctgctg ctgatccttg
     5881 ccgcggtggt gaccatgtac atcgtgctgg gcgtgctcta cgagagcacc attcacccgg
     5941 tcaccatcct gtccacgctg ccctctgccg cggtgggcgc gctgctcgcg ctgctgctgg
     6001 cccgtgccga gctggacatc accgcggtga tcggtatcgt gctgctcatc ggcatcgtca
     6061 agaagaacgc gatcatgatg atcgacttcg ccctggaggc cgagcgccac cagggcatgc
     6121 gcccgcggga ggcgatctac caggcctgcc tgctgcgctt ccggccgatc ctgatgacca
     6181 ccctggcggc cctgctgggt gcgctgcccc tgatgctggg cacgggcgtc ggctccgagc
     6241 tgcgccaccc gctgggcatc accctggtgg gcgggctgat ggtgagccag gtgctgaccc
     6301 tgttcaccac gccggtcatc tacctggcct tcgaccgtct ctcgctgcgt ctgcagcgcc
     6361 gcaccgcgac cgccccatga acctgtcgcg gctgttcatc ttccggccgg tcgccacctc
     6421 gctcctgacc ctcgccatcg ccctggcggg ggccatcgcc ttccgcctgc tgccggtgtc
     6481 gccgctgccc caggtggaat accccaccat ctcggtctcc gccagtctgc ccggcgccag
     6541 cccggagacc atggccgcca ccgtggccgg gccgctggaa cgcattctcg gacagatcgc
     6601 cggggtcaac gagatgacct ccaccagcac cctcgggtcg agccgcgtca ccctccagtt
     6661 tgatctgagc cgcgacatcg acggcgccgc ccgtgacgtg caggccgcca tcaacgctgc
     6721 ccgcggccag cttcccacca gcctgcccaa caacccgacc taccgtaagg tgaatccgtc
     6781 cgagacgccg atcctgatcc tggcgctcac ctcgaccacc ctcacccagg gccagctcta
     6841 cgacgtggcg tccacggtgc tggcccagcg cctggcgcag gtggatggca ttggccaggt
     6901 gagcatcggc ggcagctccc tgccggcggt gcgggtggag gcgaatccgg accgcctcgc
     6961 cgccgccggc atcgccctgg acgacgtgcg cacggccatc gtcggtgcgg tggccaaccg
     7021 gcccaagggg gtgctcgacg acgggcagcg ggcctggcag atcggcgcca acgaccaggc
     7081 ctacaccgcg agggaatacc gccccctcat cgtgacctgg aagaacggcg ctgcggtgcg
     7141 cctgggcgac gtggcgcggg tgcgcgacag cgtgcaggac gagcgcaact tcggttccgc
     7201 caacggccgg ccagcggtgc tgctgatcct caaccgccag cccggcgcca acatcatcgc
     7261 cacggtggat cgggtcaccg cgctgctgcc ccacctgcgc gcgctggtgc cggcggcggt
     7321 ggatctggat gtggtgatgg accgcacgcc caccatccgc ggctccctgc gggaagtgga
     7381 gcgcaccctg gtgatctccg tcgggctggt ggtgatggtg gtcttcctgt tcctgcgcaa
     7441 cgtgcgcgcc acggtgattc ccagcatcgt cgtgcccgtg tccctgatcg gcaccttcgc
     7501 catcatgtat ctggccggct acagcctgga caacctgtcc ctgatggccc tcaccatcgc
     7561 caccggcttc gtggtggacg acgccatcgt cgtgctggag aacatcaacc gtcatgtgga
     7621 ggacgggcac acgcccctgc gtgcggcgct gcgcggtggg cgcgagatcg gtttcacggt
     7681 gatctccatg agcctctccc tgatcgccgt gttcatcccc atcctggcca tgggcggcat
     7741 cgtcgggcgg ctgttccggg agttcgcggt gaccctgtcg gcggccatcc tggtctcgat
     7801 ggtggtgtcc ctcaccgcca cgccgatgat gtgcgcgcgc ctgctgcggc ccacgccgcc
     7861 ggcggccgga cgggggcgct tcgaccgctg gatcggcggc gccttcgagc gcctgctggc
     7921 cggctatcgc cacagcctgg gctgggccct ggatcatccg cggctgatcc ttgccgtgct
     7981 cctgctcacc gtcggctgca acgtgtatct gtacaacgcc atccccaagg gtttcttccc
     8041 gcagcaggac acaggccgca tccgcggctt cgtggaggcc gaccaggcca tctccttcca
     8101 ggccatgcgc ggcaagatgg aacgtctgct cgccctggtc ggcgccgatc ctgcggtgga
     8161 caacgtggtg ggcttcaccg gtggcttcca gctcaactac ggttccatgt tcgtcaccct
     8221 caaacccctc ggcgtacgca ccgagtcggc cgatgcggtg gtcgcgcgcc tgcgccgcca
     8281 gctcgcccac tttcccggcg ccaacctgta tctcaatccc gtgcaggaca tccgcatcgg
     8341 cgggcgcagc agcaactcca cctatcagtt caccctgcag gccgaccgcc tggacgatct
     8401 gcgcctgtgg gagccgcgca tccgcaacgc catgagccgc ctggacgagc tcaccgacgt
     8461 gaacaccgac cagcaggacc ggggcccgca gaccagcctg gtggtagacc gggacgccat
     8521 gacccgctac ggcctcacca tgcgcagcct caccacggcc ctcaacaacg ccttcggcca
     8581 gcgccaggtg tccaccatct acaatcccct caaccagtac cgggtggtgc tggaggcggc
     8641 gccggcctac ctgcaggatc cggacgtgct gtcgcgcctc accctggtgg cgtccggcgg
     8701 ccagcccgta cccctgtcgg cggtggcgcg cttcgagagc accctggcgc ccctgtcggt
     8761 caatcaccag gacggtgcgg tcgcctccac catcagcttc aacctgcccg agggcaagac
     8821 ccttggcgag gccacccgcg ccatcgacgc cgccctgcag cagatcggcg taccggtgtc
     8881 gatccgcacc agcttccagg gctcggccgg cgccttccag cagtccatga gcacccagcc
     8941 gctgctcatc ctggccgccc tgatctccat ctacatcgtg ctgggcatgc tctacgagag
     9001 cctcgtccat cccctcacca tcctctccac gctgccctcg gcgggggtcg gcgcgctgct
     9061 cgccctgctg gcggtcaaca tggaattcag cctcatcgcc atgatcggcg tgatcctcct
     9121 catcggcatc gtcaagaaga acgccatcat gatgatcgac gtggccctcg cgctggagcg
     9181 tagcgaaggc gtcggcgccc gcgcggccat ccaccacgcc tgcctgctgc gcttccgccc
     9241 gatcctgatg accaccctgg cggccatctg tggcgccgtg cccctggcca tcggcaccgg
     9301 cgacggcgcc gagctgcgac aacccctggg catcgccatc gtcggcggcc tggtgctgag
     9361 ccagatcctc accctctaca cgacgcccgt ggtgttcgtg ctgctcgacc ggctgcgatc
     9421 gcgcgccggc gcggcccgcg cccccgtgcc cgaccttgcc cgctctccgg ccggaaacct
     9481 gtcatgaaga cctgcctgcg ccccctgcct gtcatccttg tcgttctgct gtccgcctgt
     9541 cggatcggcc cggactacgt ccgtcctgag atggcggtgc ccaccgtcta caaggaagcc
     9601 ggtgactgga agcccgccgc gccggccgat ccggccgcag gtgacgcctg gtggacggcc
     9661 ttcggcgatc cggtgctgga tcagctgcag gcgcgcctgc tcgtctccaa ccagaacctg
     9721 cgcgccgtcg aggcccagta tcgccaggcc cgtgccctcg ccgattcggc ccgggcaagc
     9781 gggtttccga ccgtcaccgg cagcgttggc atgacccgcg gcacgagcgg gtcgagcggg
     9841 tcgagtaccg ccagccaggg ccctgcagcg gtgaccaaca gctattccct cggcgcttca
     9901 tcgagctggg aggccgacgt gtggggccgc atcgcccgca acgtggaagc ggccgatgcg
     9961 cgtgtcgagg cgtccgacgc ggacctcgcc gcggcccgcc tgtcggcccg tgccaccctg
    10021 gcccagacct atttccagct tcgctccgcc gagcgtcaga agcgtctgct ggaggccact
    10081 gtcgcagcct acgagcgcgc cctcgagctc acccgcaacc ggtacgccgc cggcgtcgcc
    10141 ggtcgtgccg acgtggtcca ggcggaatcc ctgctgcgca gcacccgcgc ccagaccatc
    10201 gatgtgcgcc tgtcccgtgc ccagtacgag cacgccatcg ccgtcctgat cggcgaggcg
    10261 ccggccgcct tcacgctggc cgagagcgat gccccgctgc cggtggccgt caccccggtg
    10321 agcctgccgt ccacattgct ggagcggcgt cccgatatcg cggcagcaga gcggcgggtc
    10381 gccgccgcca atgcggccgt gggggccgct caggcggcgc gctttccggt attggggctg
    10441 accgccgggg ccggctaccg ctcatcggat ctgtccgaac tcctgagcct gtccagccgc
    10501 tactggtccc tggggccgac cgttgcggcc agcctcttcg acggtggcgc caagcgggcc
    10561 gccaccggcc aggccgtggc ggtctgggac caggcggtcg ccacctatcg gcagaccgtc
    10621 cttacggcct ttcaggaggt ggaggacaat ctggtcgctg cgcgtctgct ggagcacgaa
    10681 ctgaccgagc agaacgccgc ggtcaccgcc gccgccgaag cggaggccat cgcgctcaat
    10741 cagtatcggg ccggcaccgt cagctacctc aacgtggtca ccgcccagac cacgctgctg
    10801 acggcccgcc gcagcgccaa cgacctcgcc gcccggcgtc tgctggccgc cgtgctgctg
    10861 gcgaagaatg cggggggcga tgtggcgcgt agcgctaagc ccgcaccctg aggccgggtc
    10921 ggccgcctgg ctgatgacgt cagctggagc ggcaggggcg cgacgtcggg gtcagcgggg
    10981 gggcagcgtg caggccagcg cgttcagcga gacgttacgg ttctgggcct ggggtgacga
    11041 cgtgggtgcc gcggatggca gggcgccaag ctggcggtcg acgtccgcga ggagcgcgcg
    11101 ctgttcattc agacgacgga atttcccccg gcattcctgt gccatgtagt cggaataggc
    11161 ggcggcgcgg tccgaggaac ggccctggct gacctgccag agctggtagc ggtggccgag
    11221 ctggtgcgtg gtgtcctgga cgcacacatc gtaggcatcg agccaggcgc cgatcagggc
    11281 gatcgaatcc ctgggctgga gtgacggacg tgcggcgagt gtctcggcat ccgtggcgat
    11341 tgccagcggt tcgcggggcg agcccagttc cggcacgtaa tcggcacccg ttcgctcgaa
    11401 gtggttccgc cagtcggtca ggaactgtcc ggcctggcgg ctgcgttcgc tcggcaactg
    11461 gttgcggaac tggcccaggg catcgacaac gctgaatttg ctggtcaggt tgagctcacg
    11521 ggtccgacgc acgcagatgc gccgagcggt cgccgcctgg gccgcgggct cgatgacgtc
    11581 ccgcccgcgt gcatccgcct tggccgtgaa ggtccgcctg gcgcagtcct cgacgctcgc
    11641 cagctgatcc ttgtccagtt cggcctggag ttgttgcacg ctacgctgcg ccgcattgcg
    11701 ctgggcgtcg agcccggatt tctggcggtc gttcaggcgc cgccgcgttt ctgctccaac
    11761 gcgggcttcc cagtccgggt atcgaaagcc gctgttgtcg gtgccaccgg ccgtgcgcgc
    11821 acggatcgga aaggccccga ggcgggtatc cgcgggggag aagtacaggc agatctcgcc
    11881 gctcggcggg ttgttcaggg tttcggggag cgtatagaca cgggtctggg cgtctacccg
    11941 gctgcccccg cccagggatg ccgtcctggg ggttccgctg tcgtcgacga aatcggcggc
    12001 cagattgatc tgcgcgctcg caaactgtct caggcgcgga tgctccgggt cgagtcgcag
    12061 cacgatccgg ttgccgtcgc cactgacctg ggtccacagg gagtccgaca cgctcgtgga
    12121 ggcgcaaccg ccgcacagca ggcttgcgat gaggacgttc agagtagtgc tgttcaggcg
    12181 catcattggg tcacgaagcc cgcttcgcgc aaggcctggg gagagaggtt gatggtggcg
    12241 ccgatccggc tggccgtcat gatgtccatg atgcgcagca cctggggcgc ccgcgggccg
    12301 aggcggtcgg cttgtgcggt caggatgccc acgcacaatt cggtggtacg gcgctcggtc
    12361 gcctcttcga tttcgttgag ggcggggcga ccttccacac gcacccgctg cgccagttgg
    12421 cgcaggcggc ccggctggag cagctgatcc tgggcacggc tcagacattc cgagttgctg
    12481 ccttccagca ggcccttcgg actctcgccg gcgatcatgc gctgtacgta ggcaaaacgt
    12541 gagagggtcc attcgacccg gtctgcaacg gtggcgcgca aggtgttgcc gagttcaagg
    12601 tcggggtctc gggaaacgcg ctgccattcc tcttcggaca ccccattcag gtagctcgac
    12661 gaggagtgca cgtgaaactc tcctccttca tccacctcgc ggatcacccc gcccagaaaa
    12721 gcggcggtgc atgcgctgat gcagtgatgt cccttggcga cgcggaccca tgcgccacgc
    12781 gcgcgcagga cagcgccgac acgctggcct tccgtggaac tgccgccatt ggagttgagc
    12841 aggacctgca cgtagcggtc ccgttcgaga aagtccttga ggcggttcgc gtcacccgcc
    12901 gtgaaaccgc aggattcatt ccgggcgcag gggcgcttgt cgtgaacgat cagcagtttc
    12961 aggccgttgt cgagtgtgac aggcgtgaag tcgagtgcca ccgcgggaag cggggcgacc
    13021 gccagcagga cggccagtcc gcacagacgg cgggcgccgg caagaaaacg cgcgttcaag
    13081 gcttcagctc acggcgggtg cccagccgcc gtcagcctgc tggcacatct tcacatagac
    13141 gttttcaccc ttgccacggt cggccagata gtcggtgttg gtgacgcaca ggctcccggc
    13201 ggcatcccgg ttgcgcggac ccggcgtaac cgtgccggtc aatgtcggcg cctgatcgtc
    13261 gcgataggtc tggactcggc ctgaccgggc ggcttccagt agcgcgatct cccgcgcccg
    13321 tcggccgttt tcgctcaact tggcatagac ggtgccggca agggcgtccg cgcctacgca
    13381 accgagcaag gccagggtga tggccgtgtt cttgttcggc ttggactgcc gcttcgaaac
    13441 ctccttgccg gccatgtaac cgagcccacc gccaatcccg cagatcaata tccgccacgt
    13501 gacgccaagc tggtcctggg atgcctgagg ggccggggcg ctgccccggg ggacaggtcc
    13561 cgacacgggc gacgacgagg gcgtcgcata aggggaagac ggggtggtac aggcgccggc
    13621 gaaaagggcg gcggccacca tcagtgcgac gctgcggcag cgggcggacg ggatgtcgtg
    13681 gagcgtaagt gacatgggca atcttcctgc gctggaggtg ggaacaggac cgggttcgtt
    13741 catgtgcgcc gccaggttca ggtcgaggag gatccgtgac cgtggtgggt tcctgaccgg
    13801 gcgtgtcgaa gcagtatgcc agcttcgcgc aagcttccag tgtggagaat gaccgcctgt
    13861 cgatgcaatg aagcccgtcg tgtccgtgtc gcagacgttg ccggactgcc cgtccatggc
    13921 ggcacgcgcg atctgcggtc cggcgatcag gagggggcgg agggccccag cggcggcagg
    13981 ccgtgacagg cccgggcccg ttcgcatttg tcgtcaccgg cctgcacttc acggcaggcg
    14041 tcggggcgat gcggatagac cgtgcagcgt acgtccgtgc ctacggcgcc gtgcaacgcg
    14101 gcgcagcggg gggcggggcc ctgcgtgccg gccatgcagc tcatccaggg attgatctgc
    14161 tcgacatagg cgtccggtag cgtgcgggcg agggcttcgg accagtagaa ggacacccgg
    14221 aattgggcgc agcaggcgcc gcatgcctgg cacgggctga ggtcgtcggg gggcggggtc
    14281 atggtgacgg gggagttggg ccgcggcgga ttgtgccatt ccgtcgcatg gcggagaagc
    14341 cgtcaggccg gcgcccgatc tgaacggaaa tccatctgga ataaaatgtg gcgatctgta
    14401 aaattggcgc cgtcaatcaa atttgcatag ttgctttgcg tgccctgcca ggtgcgtgca
    14461 atgcttccgc ggcattgctc ccattcctgc aacccggaat ggtcgccgca gcgtcttcca
    14521 tctatccctg tttccgactc acttccgatg aacacgtttt tctcacgcct gatgacgctt
    14581 ggcgtcgtca ccctggccgg attgggcgtt gccggtccgg ccgcagccaa tcccttccag
    14641 atcgccacgg acggcagcgg tctcgcgggc gcccagctgc tcagcagctt cgacctcggc
    14701 ggacacggct tcgtgttcca gtacatggat ccgaccagcg gcctgcccat gttccaggag
    14761 aacggtgcgt tccgcatcgt ggcttccgac ggggtctcac cgatcggcac gaacgacctg
    14821 accctgacct atgccctgtg gggaaccgct gatccgttga gcgggctgat cagcctgatc
    14881 ggcggcggct tcgacctgta ttcggacagc agcttcgatt tcggcaccag cacgggcatc
    14941 tacggcgccg acgacggcac ccatgtggcg agctttctgg tgacgggtgg gagcggcgat
    15001 cccatgggca cccatctcgt gagcatgtcc gcgcaactcg tggcgggctc cctgttgccc
    15061 ggctacctgt tcaccgccga ccatcgggac atgagccagc tggccagtgt gacgctcaac
    15121 gccagcttcg tggacaacgt ggcgacgcct gacgccacgg cggtcgagga actggtctgc
    15181 gaacaggccg ggctggcggc tctgggctgt caggggacgg cctatgccaa ccagattccg
    15241 tattactttc tcgtggagga cagcgcgtcc gtcacattga acgccgttcc ggtgccgggc
    15301 agtctgtgcc tgggcgcact ggggctggcg atgctgggca tgcaccggcg gcggatgcct
    15361 gggtaatccg cgtgggtgga ggcagggcag cggccggagg gggagacccc tccggccgtt
    15421 gtcctttcag gtggcgaacc gggcgtccgc ccgcaagcca gtcattcaag caggccgagg
    15481 cgaccggcca gccatttggt ggtggtggcc tgcagcagga tggtgaccag gatggcaatg
    15541 aaggtcaccg aggcgatcag cggcgcttcc ggcgcgccca tgcccatcag caggcccgcc
    15601 agcgcgccgg ggatcacgcc ggtctcgcgg gtccagcaca tgaacagcat ctcctgccag
    15661 ctccagcgcg ccttgcggtc gggcagggcg cacaggaaca ccgtcgcggg ccgcgccacg
    15721 aacatgaaca ctgccaccac cgccagccct tcccacaggt gggcgcgcat cagggcgaaa
    15781 tccacctggc tgcccagcag gatgaagatg aacatgcgca tgatcagggc cgtgatggcg
    15841 acgaactcct ccagcttgtg ctgctcttcc tcacccatct cgaagccgag cacgtccttg
    15901 ttgccgatca ccatgccgaa cacgaacact gccatgaagc cgctggcgtg caggccgtcg
    15961 gcccccagat aggcgccgat cactgccatc agggacacca ccggcgcgta ttcctccagg
    16021 aatgcgtact tctcgtgggc gatgaagagt gcggcaagcc agcccagcac cagtccggcg
    16081 acgaggccca cgccggcctg tactgcgagg tcggcgaggg acgcggacag ggagaattcg
    16141 ccctggccca tggccagccc gagcaccgca aaggtgagga tggcgccgac cgcgtcgttg
    16201 aaggccgatt cagacatgac cgtctgggcc acccgctcgc ggatctgcac ctgacggaac
    16261 accggcacca gggtggccgg gtcggtggag gcgatggcgc tggccagcag cagggcgacg
    16321 atgaagggga tgccgaggat ccactgggcc gcgtagccgg tgatgaaggc ggtgatcagc
    16381 acgcccaggg tggacagcag gacgatggac acccacacgt tcttgagcac ggccaggcgc
    16441 agggaggcgc cgccgtcaaa gaggatgtag gacgagccga agatcaggat gatctggttg
    16501 agggccgaat cggccttgac gtccacccag cccagggccg aggggccaat ggccatgccc
    16561 acgagcagga agaccgccac gtcgggcatg cgcagcttgc ggctggcaaa gcccgagacg
    16621 gagccgatgg cgaggatgat gccgaaggtg acgaggacgt gtttggcgac ttcgatcgag
    16681 tgggattcca tgatgggcgc agacggaagg agacgggcag tgggtcgggg cggaagttgc
    16741 cggagcaaac ctgtcgattt cctgacaatt tgcccgccgg gcttggcttc cagggagcgt
    16801 caagcaagct tcgttcctgc aggctgacat tctaagggtt tgacgacccg cggcgcgcgg
    16861 ccatgcgcgt gccgctgtct cccgcggtcg gtgttcagcg attctccagc cggctgtagt
    16921 ccaggattga cgcctcgcgg gggctgctgc gctgccaggc gtcgtgcagc gcgtcgctca
    16981 ccggccagaa gcggctgtcc gtgcggcgga tgccgaagcg ctggaccagc cgcagcagat
    17041 cgtcctcgcc cgtcaggcct tccaccgcat tgacgaagcg cggcagttcc gccgggtcga
    17101 gctggaagat cgcgttcggg taggcgccgg caaagccggg cagggcaagc agggtgtctt
    17161 cctccggcag gcggcgcgcg tcctcgttga acatctgggc cacgttgctg tgggcgctgt
    17221 tgcggatcag ggtcaccggc tgcacgctgc cgtcggcctt gcgcagggcc agcagactca
    17281 gttcgggcat ccacgaggcg ctgcggccct tcaccgccga caggcgggcg aggagcgcgc
    17341 cctgcgccgg actgaagccc gtcccggcaa aatcgagccc ggcctcgcgc acggggcgaa
    17401 catggcgctg cgccagccgg tacagctcgc cgagactgtc cggtccgtgg aagcgcacgc
    17461 cggtctcgcc cggatagtag gccgaggtgt cggcaaactg gcggatgcgc ggttcggagc
    17521 ggccccggta ccagtggtcg agcactttct ggcggtcctt gaggggcagc agggtgagga
    17581 agttctgttc cccttccatg cgcaggaagt ccatgtagag ccgcgtggcg agctggtggc
    17641 cgacgttgcc atacacgtcg aagccggcca ccagcaggta gtgcatgcgc tcgaacaggg
    17701 gatagcccag cagcagcacg gtctgcggcc gctcgccggc cagcccgcgc accaccgagg
    17761 cgctgtcgaa gtggcggaac accgtcagcg ccgcgttggg attgcgcccg tcgccggtcc
    17821 acaggtcttc cagacgcggg cgcacggcgg tcttgccggc catgaaggcc gacttggtgt
    17881 tgaggtagtt ccgttcggcc cgcgcgtagg cgagccaggc cagggcgccg acggagctgg
    17941 aatcctcggc cggcatgcgc aggttgggct gggtggcgtc cagcaggccc tgcacgttgc
    18001 ggctgacctc accttccggc gcatagaaga gcacccagaa gtggtcgtcg atgacgttca
    18061 gcgccacctg gccgcggcac accgggccct tcatgaagcc catcatcgtg aacttcgcgt
    18121 cgtccagcat gaagcggtaa cggctgtcca cgggcagggc gcggaaggtg gcgaagggat
    18181 tggccgccgt ctccggcgcg tagccgggca ggcgatccac ggtggcaggc gtgtcgaaga
    18241 accatttgcg cagccgcgtc agccgtgcgc tgtcgaggac caggggcatg taggtcttgg
    18301 ccaccggcgt cgcctcgttg gggcgcaggc ggtaatacac gcgggcaacg ccggggtcgt
    18361 cgtagggccg tcgggtggcg atggggtcga tgggcgcgcc gggcgccgtg cggctgcgca
    18421 ccagctcgaa gcggatgtcc ggcgcctcgt cgaaatacag atggccgatg taccagtgct
    18481 cgtagatgta gcgggcggcg agctgggcgc cgggggcgtc ggcgttgagc agggcttccc
    18541 attccgccac gcggcgggcc acggcgggcg gcgccggggg gggcggtgtg tacggcgctc
    18601 cggcttcgat ccagcggctc aggatgtcgt gttcggcgct gcgcagggcc ggcagaccga
    18661 agggcatgcc ccacaggggg tggctgcgct cataggcgcc gacgccctcc agcggcgcgc
    18721 acgcctggct gcggtccagg gagaaatcga agtccttgtc cggcagcgcg ccgctgcccg
    18781 gacccggctg ggcctgcttg agggcgagca ggcgcagcag caggctgccc tcccggttcg
    18841 cctcgggcgt cggcgagcgc tcgttgagca ccgggaagaa gccccgctcg cgccacgccc
    18901 gcgtgctgag ggcgtcgaag cccaggcggg tgggctgcgc tgcggtgagg cgcgtcgagg
    18961 aatacaccgt ctcggcgctg gcgccgcggg tgatgccgtc gtagcggctc aggttgagct
    19021 ggcagggggc gtcgtagcag gcgtggcagg tcacgcagcg ccggtccagg atcgggcgca
    19081 cctgggtcca gtagtccggt gcgggggcga ccggcgccgg cgcctgatcg tagcggcccg
    19141 gatcacgcgg accgaggcgt tcgtccagac gatgggcggc gacggcggta cagccggtga
    19201 cgaagacgat gaggagggcg aagagcgggc gcatggggca gacggcggcg gtgtgcggtg
    19261 gggaatccgg gaaggcccaa ggatatcccg gaagacggcc cgccgagcgc cgcgcgcccc
    19321 atcgccgtca cacaagcccc gcggcaaaca cgccccacag tcccatcagc accaggtttc
    19381 cggcggtcca cacgccaaag cgcggcagca cccggttgtg ggtgagctgg atcagcgagt
    19441 ggccgatgcg cagggccacg tagacccacg ccaggatcgc cgccgcctgc ggcacgctcg
    19501 cctgctgggc gaaggccagg cagcacaggt ggaagagtac cggcacctgc agcaggttgt
    19561 tgaagttgtc catggcctgg gtgtcgtcca gcgcctgggc caggtcgcgg gggcgggcga
    19621 gggtggcgag gggaatgcgc cggcggcgga tctcccgcac gcggcgccgg gccgccagca
    19681 tccccacgcc accggtccac gcgatcaggg ccagcatggg cccgtaaagg cttgaagtca
    19741 tcacctctcc tttccgaggg cgtgggggcg ggcttcaacg cccgtaagtg gtctgcacga
    19801 ccgccgagcc cagccggttc atgtggatca tgtagcccat catcgccggc gaggcgtccg
    19861 gcggcacgcc cagccgctcc accttgaggg cgtgcaccgt cagcacgtag cggtggggcg
    19921 gcgcgccttc cggcgggcag gcgccgccgt agccgggtgc accgtagtcc gtgcggccct
    19981 gcagcgcgcc cgccggcagg ccggggcggc cggcgttgcc ggcgccttcg ggcaacgcgc
    20041 gctggtcggc ggagatgtcc accaccaccc agtgccacca gcccgagccg gtgggcgcgt
    20101 ccgggtcata gagggtcacc gcgaagctgc gggtgccggc gggcgggtgg tcccagcgca
    20161 gggcggggga aaggttctgg ccgtggcagc cgaagctgtc cagcacgtgg cgctccggta
    20221 ggggcgccgc agcggaaaag ctcgggctgc tcaccgtgaa gtcctcggcc agcaccggcg
    20281 tagcggtggc gaaggcgagc agggcgaggg atgtggcgaa ggggacgaag cgggtcatgt
    20341 cgggcactct ccagggcaga cgggccgccg gatgaacggc ggcgggcggg tcgggcggct
    20401 tcgggagggc ggtcgggccc cgcagggcgc ccaggccggc caaagccgct gactgcatcg
    20461 tgccgggccg cgccgcggcg cgctaagccg tctccgtcag atcctgtgcg tcgtctttca
    20521 ggcgggcgcg caccaccgac ggcggcacgc cccagcgttc ctgaaaggcc accgtgaagc
    20581 ggctgtggga cttccagccg cagcgcgccg ccacctcgcc caccggcagg cgggtggtct
    20641 ggagcaggcc cagcgcggtc tccaggcgga tgtcccggac cagcgcggcg agggtcgtcc
    20701 cgctcccttc caggcgccgc cgcagggtcg attcgctcac atggaaggcc gtcgccaggg
    20761 tcgccacatc ccagtccgcg tggagccgct gcgccaccag gcggcggacc cgctccggcc
    20821 agggcaggga ttcggccggc gcaaagcggt ggccccgctg ggcgaggagg atcagcacct
    20881 ccagcacccg gtgacgcagc agggcgccgg ccagcgggcg ggccgccacc ggcggcaggg
    20941 tgcgctgcac ggcggcgagg agttcttcat ccaccgccag cacctgggcc gcgtccacct
    21001 ccggcaggcc ggcggcggcc tccagggcgt ggcattcgcg gatcagctcg gcaggaaagg
    21061 caatggccag cgccgcgtat tcgcgccggc cggcgggatc gttggtcacg tcccactggg
    21121 tgccgcgggc cagcatcacc ccctgctgca cgtccgccga caggcgcgcg tggggcgcat
    21181 gcagggtctt gcgcccgccc agcacggcaa tgaaggcgtc gtcccggatc gtcagcgtct
    21241 tgagcgtgta agcctgcgtc gccaccacgc gcgcgccgtg cgctgcatcc atcggagggg
    21301 ctcctggggt ggaaaaggcc cgatcctaca ccggcgccga cagtcctcgc ccgccgcgat
    21361 ccgccaccgg agccgcgcgc tcggcgtgtc ccggacgtcc ggcccctgcg gcgctcactt
    21421 catcgagtgg aagccgatca tgttgccttc cgaatcctgg gcgagggaaa agaagccgtg
    21481 ctcgccgatg gacgtcttct ccttgcacag gcgtccgccg aactcggccg cgcgggcggc
    21541 ctgcaccgcg cagtcctcgc aggcgaaata caccagcgtc ccgccgccgc ccgaggccat
    21601 gccctccatc cggaccagca tgccgccgga accgtaggtg ttcatccccg tgtccttgtc
    21661 catcggaaag aacagcatgt ccagatccat ctccggcgtc ggcgcaggca tcctgaccag
    21721 cgtcgtgccc aagacgttct cgtagaacgc gcgggcgcgc gccatgtcct gcacgtagat
    21781 ctcgaaatga acgactggat tggtgggcat ggcgtgtttc ctcttggaaa gtctgttgtc
    21841 gtgttaccgc ggcgtccgga tgtgcggtgg tcggcggttt gggatgtgca acgcgggcgt
    21901 tccacctgcg tgcacggggc agaaggccta ggacatcaca gggtaacggg cagacgtgtc
    21961 acgagaacga cggcccgtct gcacacaggc gacccggcca tcggtacgag gcgccctggc
    22021 attggggttt gcatcggcct gttagagcaa gattaaattc cactttcgcc cgcctgcgtt
    22081 gacttcttgt ttcgccttcg ctcacgccaa accttcagca agaactcaat aaatgaactc
    22141 accaaagaga caagcatcgc aagaatcgcg gcgtcatacc agtggaaacc tgttcgtttc
    22201 tcctgggaga ctggcttgac ggacagattc tcggcttttg aaaacacctt tacatctgcc
    22261 ttagcggtcg ttagaaacac aaggctatcg gcgtcctctg agttcaatag ggcgtaaata
    22321 aaccgtttgg aattagtgtc gtttccctct tccgtaactg ccccaaattc cttgggcgga
    22381 tttgtgttgt gatttacgga taggacgcga aaatctttat cagtcgcagc gaactcaaac
    22441 agaacaccca agttctttaa cggaagtgag ccggagttcc agatccgtac gcggactgca
    22501 aacacctctg gcaccgaaat gtcgttaact ttgacagagg ccgttccaat agatgtcttg
    22561 tccaaatagg ccactggctc ctctatcgtg tagctaagct gacttgactc agttaaaagc
    22621 ttgggcagag cataggtaaa gatggcgatg acaatgccaa cgacgagtgg gacggctaat
    22681 aggtctctaa tccaggagcg attgttgtct gccatttttg taatgagcta gtgaaatcta
    22741 atgtgaaggt aacagacttt gcgcggcttt ttgcgcaagg ttcgcgttga tcacagggtt
    22801 agcagtctgg gtcaaattca tcatcgagct ttctcggtat gcagcctcca catgcagaaa
    22861 ctgaacccca ttcgacaacg tgatcgggcc agccgtagag ctgcccagga gttgaaacaa
    22921 gggagcgaac ccacgaaaca aattgatcga aaaatggcgc gttcgagttg cttcggtgcc
    22981 cctgcatttg gtggcaaata gggagtaaat aatcaggtag tcttctggct cgactgccga
    23041 agtaatagaa atctttcgat atcaagacat tttcgccgct gaggtcagtg gcgacgttgc
    23101 caagcccgtg aacgcctgcc ctttgaacag cgctgccgct ggaaaaatcg taaatgcaat
    23161 cgccaaggcg atcttggagc gcagagcttt gaatgttcgg aatacggtgc ggccattgag
    23221 ttctggcatt aatgtcgtag tcgcgcaaag atataacctg ctcgacacgc atcgcatata
    23281 tcaagtgccc gcttaggtct ccgcttggcg cgttcttgga gccaaggccc gcaacccaat
    23341 catggggttt cgccactcga cgaatgcctg gtttgcaaat ggcgagggag cacatgccgt
    23401 ggaatgggtt cggggcagct ccgtcatcga cggtgatcgt gtaggtgaat aatcgtgaca
    23461 tatgactact ggtgtgttag ctaacggacg ctccgccgct aggcggagcg tccgtgttga
    23521 gcggcgggtt agccccgatg gccgacagcg tgcgttccat cgtgtgaagg gcaaatgaca
    23581 tggtttcttc caattcaaag cggcgctccc gagaacgagg accaaattcg cggaacattg
    23641 gatgatgggt cgctatttca aatagacgaa cgaaacgctt gagtgcatca ggcgcatcaa
    23701 cagatcgaga ctcaaacttt cgatacacat cgagtgcaat ttcgtagagc caagggaaat
    23761 catccttgaa aaggctacac acaatgagaa gaacaaccgg gtcgccagtt tcttttccgg
    23821 ctatgtgcat aattttatca aggaagtgtg ggtgaatttt acgtcttctt cgaatttccg
    23881 ccgaggactc tgggtcaata cgtcgttcga ttcgccctgg taaatcttga aacatcactt
    23941 tgatttcctc gaaaatcttg gcgatggatg cttcgtccgc ttcttctttt gttctgattt
    24001 cagcagattt ggcttcaaat aggggcgaaa ttttttgctt gaatgcctca acctgtattt
    24061 tgacgacatc acgatctggt tcagcgttcg gaattctccc aacaagctga atgaccaagc
    24121 tcgtcagtga gtcttcagca tcatcacagt tctgaaactg agcaaacgga ccagtgctgg
    24181 cgacgtggag ggggataccc agtgcaaccc catgaacagg ggtgtccaat tttcccttgg
    24241 cgaccccggc ttcatatagt agccacgggc gatttacgct atttggcgta agcaagcaga
    24301 ccacatccga agctgtttcg agttttttca tgatttcggg ataccactca acgccgtact
    24361 cgataccttg gttgcccttc ttgtcggaag agcgaaacga tttcaatacg ccagcactta
    24421 cgctgctcag gagccgacta aaggcttctg ctatttcagc atctctagtg tcgtggctga
    24481 tgaacacaag ggccttccga tgtcgctggc tatcttcatg gggcgtgatt tgttggggtt
    24541 gttgatccaa gaggtatctc caatttggtg agggctaacg caaggtagac gatatccgac
    24601 gcccttggcg tcctacacga ctctcgccca agctgccagg atgtcccaag taactgcatt
    24661 taaaaacaat cgatcccgcg ctttccgtca tgtcgtcgtc tgcccttcct gaggtacttc
    24721 ctgcatcctc tcggctgctg aatcaggttc gacagcgaat cagcatccag cctgaaagca
    24781 gatcaggctc acgcgggatg aattaaacgc tatctccgtc gtcatgacaa gcgaccccca
    24841 caggattggg cgatcaggca ttgatggatg tccgaacgca tgcgcaatcg aagcgccatg
    24901 tctcccgatc atccgttccc actccgcgga gtgaatgcca tgcggtgcga tacggctatc
    24961 gacctgagct gattgaccac gcagccgtga ggtgggatct ctcgctgcgg taaagtgctg
    25021 atgcggcccc gaccgttgca atcgcaatgc ccacacacac tgcgccgcgg tgacaagggt
    25081 ctgtccgtgg gccggatcga tgttttgcgg cccgagatcg ttctgcgcgg acctcaacag
    25141 acgatgtgga cgtcgacaac aaagctgttg atagctaa
//
LOCUS       DAOQMM010000046        24682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_636_length_24682_cov_47.573476, whole genome shotgun sequence.
ACCESSION   DAOQMM010000046 DAOQMM010000000
VERSION     DAOQMM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24682
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_24682_cov_47.573476"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            919..1608
                     /locus_tag="PLH85_09245"
     CDS             919..1608
                     /locus_tag="PLH85_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64788.1"
                     /translation="MSDVVYFTIGLNLAMNQPQKFVDLIEKLIRDARVILASARGGFI
                     EDREALRRWSNELILLRNLGGTMLSPWARRLAHDGVLIPVEYVQRPLAALETVKYATD
                     NGLLTSYRDLVIAEAFTDLYEQGQYLFSQGYFLAAGVIFRAVLEERLRELCLAADCMP
                     EKERPTLGDLNQALFRCESVPYDKAMMLHVTALAAVGNDAAHNSDSLQKEDVERLMRG
                     TLEFLSRYSST"
     gene            1685..2236
                     /locus_tag="PLH85_09250"
     CDS             1685..2236
                     /locus_tag="PLH85_09250"
                     /inference="COORDINATES: protein motif:HMM:NF037888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64789.1"
                     /translation="MTAHSAQVTQEFCKLCDWAFQVWLNHRSMFDDNPRAEELQESMA
                     ADALARLRTISHEYSLLQIAKLHDPVVVAGQVTLGIEYMVKYGAWDAATSATLSQLTE
                     KLNSFAKGLRAVRNKALSHNDLAAVLSNATLGEFEKDEDIQYFATLQQFVDVVHGEVI
                     GGPWPFDDLAKNDVVALMAMVKS"
     gene            2233..2526
                     /locus_tag="PLH85_09255"
     CDS             2233..2526
                     /locus_tag="PLH85_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64790.1"
                     /translation="MKKIAPDAQPIRADLREKPRRPLNSNVVRHRAMSPRNRKLLAAT
                     AGLVFVLAGVVVKFAGQGVVAAIGDTFVATNSIPGWSAPQDRSSFYALFVLGL"
     gene            2615..3025
                     /locus_tag="PLH85_09260"
     CDS             2615..3025
                     /locus_tag="PLH85_09260"
                     /inference="COORDINATES: protein motif:HMM:NF026937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm63 family immunity protein"
                     /protein_id="HNB64791.1"
                     /translation="MSNLASLSALAQEFHAMAAGLDVEPYFKSFHTTPQHDGSPHIEV
                     ADGKFQFVVTERGSEFERIKNLSADDVLYLLFNGITQHMATRYELKNRVPETDGRSVW
                     FPYQERLMERLNPVWGERLRKEHQRILENYPLQP"
     gene            complement(3097..4305)
                     /gene="nifS"
                     /locus_tag="PLH85_09265"
     CDS             complement(3097..4305)
                     /gene="nifS"
                     /locus_tag="PLH85_09265"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNB64792.1"
                     /translation="MEPIYLDNNATTRVDPAVVDAMLPFFTEHFGNASSIHAFGSKVG
                     AALKKARGQVQALIGAEHDSEIIFTSCGTESDSTAILSALKAQPDRKTVITTVVEHPA
                     ILTLCEHLEKEGYTVHRLTVDSKGRLDLEAYKALLSDDVAIVSVMWANNETGTLFPVV
                     EMAELASAKGIMFHTDAVQAVGKVAIDVKSSKIDMLSLSGHKLHAPKGIGVLYLRRGC
                     RFRPLLRGGHQERGRRAGTENSASIVGLGQACELALANLDKENTQVKALRDKLEQGIL
                     SQISHCFPTGDVDNRLPNTSNIAFEYIEGEAILLLLNKLGIACSSGSACTSGSLEPSH
                     VMRAMGIPYTAAHGTIRFSLSVDNTEAEIDRVIAAVPPIIAQLRKLSPYWGENGPAAN
                     PAETFAPTYA"
     gene            complement(4349..5245)
                     /gene="nifU"
                     /locus_tag="PLH85_09270"
     CDS             complement(4349..5245)
                     /gene="nifU"
                     /locus_tag="PLH85_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNB64793.1"
                     /translation="MWEYSDKVREHFFNPRNSGPLEEANGIGDVGSIQCGDALRLMLK
                     VEPETEVIQDAHFQTFGCGSAIASSSALTEIIKGMTLDEALKVSNQDIADYLDGLPPE
                     KMHCSVMGREALQAAIANYRGEEWSDDHEEGALICKCFAVDAVMIEDVIKANNLNTVE
                     EVTFYTKAGGGCAACHEGIEEILARVKAELPGTGEVSPPPACPATPEAPKPPAAKLTL
                     VQKIKKIEEVLESVRPMLQRDHGDVELADVQGKKIYVHLKGACSGCMMEAATLGGIQQ
                     KMIEALGELVQVLPASHMPVEA"
     gene            complement(5258..5581)
                     /locus_tag="PLH85_09275"
     CDS             complement(5258..5581)
                     /locus_tag="PLH85_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNB64794.1"
                     /translation="MINLTPAAVKAVQRFIRGSETPVAGLRLVVSGGGCSGLQYGMKL
                     EAAKAEDDWELDIEGVKVLVDPMTFPMVDNVTIDFVDTLTHTGFKFDNPNASAQCACG
                     SSFSV"
     gene            complement(5702..6124)
                     /locus_tag="PLH85_09280"
     CDS             complement(5702..6124)
                     /locus_tag="PLH85_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64795.1"
                     /translation="MSALPSPRLILLHKHGTSGRVRFLCLASGVLAFAPLPPLAALRD
                     EAYSPKVQVHPTAVVREAEVHLGMAEGTIQTEADFQAWVDTPAGDVPVLMGLFMGIEP
                     PFEVAARSQGRFIALTESRHLSDVERQLLRRAYEHVLG"
     gene            complement(6132..6479)
                     /locus_tag="PLH85_09285"
     CDS             complement(6132..6479)
                     /locus_tag="PLH85_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64796.1"
                     /translation="MALPVPDPALGARICEALRAQVAKLGLSIGDEPVWADAVFETRT
                     DPFSQEESRVGTWSGGLRYGNVTFFADGRIFAEYQVLLPLPDKADSYLESVQVWGSAE
                     RLRGDAVIAEFAK"
     gene            complement(6479..6760)
                     /gene="fdxB"
                     /locus_tag="PLH85_09290"
     CDS             complement(6479..6760)
                     /gene="fdxB"
                     /locus_tag="PLH85_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HNB64797.1"
                     /translation="MSQITGLTRGGAEWTPQFVTELNQAKCIGCGRCYKVCPRNVLDL
                     VERELDDDDDSEDDNMMVMSIANAMDCIGCGSCGRVCPKDCYTYAPASV"
     gene            complement(6766..6987)
                     /locus_tag="PLH85_09295"
     CDS             complement(6766..6987)
                     /locus_tag="PLH85_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HNB64798.1"
                     /translation="MSDVLTPEDLQALDKEVKKLKRIASEWASQMHDLVEDRLPAAYQ
                     EIPTLSQSTYEACKAWADANARLAAAQKG"
     gene            complement(7002..7460)
                     /locus_tag="PLH85_09300"
     CDS             complement(7002..7460)
                     /locus_tag="PLH85_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HNB64799.1"
                     /translation="MTATTADPILETDFVKEMARQMRALDTYGTYTGWSVEKILAPYV
                     LTKERKAEIPLIGDPDDETISRVKAFYNSIAVLIEKEAGLLAVPLIHLTHEGFGRALI
                     TVGRLVAVDKTLRDVHRFGFASLEKMKEEGDKILKGAVARITEHRDVADA"
     gene            complement(7441..7929)
                     /locus_tag="PLH85_09305"
     CDS             complement(7441..7929)
                     /locus_tag="PLH85_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNB64800.1"
                     /translation="MSSAHIEHRGVVRRVAGGKVFVAMETSGCASCDHGAGCGVGRLA
                     QGQDSTLLTVRADADYKAGDAVQVTLPERGMTLFAMLGYLFPAFVMMLGAALGMAVEG
                     SDAAAALGAIGGFLGAMLILRILISLMPDLMPTPQLSPIPLAPAASTLSPLEFHHDRH
                     DR"
     gene            complement(7926..8405)
                     /locus_tag="PLH85_09310"
     CDS             complement(7926..8405)
                     /locus_tag="PLH85_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNB64801.1"
                     /translation="MTPRRLQLVWSIKKEPAAVLKVAFATSDRVRVDQHFGAAESFAL
                     YEVTPDKATLVGVAEFPEEAMDGNEGKLAAKVNFLEGCAAVYVMAIGASAIKQLLAKG
                     IQPIRTNEVDAVFDLLADIGKAMTEGGVPWIDRALALQAKAASTDRFNSMEEEGWQG"
     gene            complement(8368..9777)
                     /gene="nifN"
                     /locus_tag="PLH85_09315"
     CDS             complement(8368..9777)
                     /gene="nifN"
                     /locus_tag="PLH85_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HNB64802.1"
                     /translation="MAEIVHSKKALAVNPLKVSQPVGASLAFLGIARSLPLMHGSQGC
                     TAFGKVFFVRHFREPIPLQTTAMDQVSTIMGADDNVVEALRTLSDKSKPDLIGLVTTG
                     LSETQGTDIRRAVRNFREAHPEFAHVAVVPVSTPDYVGCLESGYALAIESLIETLVPE
                     SSCAGRRPRQVNVLASGMLTPGDIEAIKEWVEAFGLRPVVVPDIGDSLDGHLVEAEYS
                     SLTLGGTPRQEIESMGESVATLVIGPSLYRAADKLKARTGVPDFRFAGLMGLDACDAL
                     TQALAEISGRPVPERIDRQRAQLQDAMVDCHFMLGFARVALAADPDLLGMQAHFLTGM
                     GAEIVAAVSPAKADSLAELPIEQVTVGDLEDLELQARKRGAQLVIANSHAAETARRLG
                     LPLMRAGFPQYDLVGGYARTWVGYRGSRQALFDLANLMLSQHHELEPYRSVYWNESRD
                     AGGSCEHHDTPSAATGLVH"
     gene            complement(9788..11188)
                     /gene="nifE"
                     /locus_tag="PLH85_09320"
     CDS             complement(9788..11188)
                     /gene="nifE"
                     /locus_tag="PLH85_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HNB64803.1"
                     /translation="MKASEIQALLDEPACTHNKKEKSGCARPKPGATAGGCSFDGAQI
                     ALLPIADVAHIVHGPIACAGSSWDNRGTRSSGATLYRIGMTTDLSETDVVMGRGEKRL
                     FHAIKQAVETYSPAAVFVYNTCVTALIGDDVEAVCKAATERWGTPVVPVDAAGFYGTK
                     NLGNRLAGEAMFKHVIGTREPEPAAPRVDGRPTYDVNLIGEYNIAGEFWHVSPLFDEL
                     GLRILCTLSGDARFHEVQTMHRAKVNMVVCAKALLNVARKMQDTYGIPFFEGSFYGVQ
                     DMSTALRDFARIIGDPDLIARTEAVIAREEAKSRADLDPWRERLTGKRVLLYTGGVKS
                     WSIVSALQDLGMKVVATGTKKSTEEDKARIRELMGDDTKMIDDGSPKALLSIYKEYKA
                     DILIAGGRNLYTALKARIPFLDINQEREFGYAGYDGMVELARQLALSMNSPVWEAVRR
                     PAPWAKQSGPGTVLAA"
     gene            complement(11426..11695)
                     /locus_tag="PLH85_09325"
     CDS             complement(11426..11695)
                     /locus_tag="PLH85_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNB64804.1"
                     /translation="MQIGVAYSEPGQQIWLNIEVPDESTVEDAIQRSGILKQFPHIDL
                     AAQKVGVFGRLVKLNAALKPGDRVEIYRGIIADPETVPRRDQDEE"
     gene            complement(11709..12401)
                     /locus_tag="PLH85_09330"
     CDS             complement(11709..12401)
                     /locus_tag="PLH85_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNB64805.1"
                     /translation="MSEYRDICTDGLWKQNIVFSQILGMCPTMAVTTSGTNGLGMGLA
                     TTAVLLVSNALVSSVRNVTTPQVRIPVYIVLIACLVTLVDLGINAWMHDLYKVLGMYI
                     ALIVANCAVLGRAEAFAARKPVLQSAMDGLWMGLGFTFALTTIGLCREFVGQGTLFSQ
                     ASLLLGPSFSFLEINALPGYGGTLLMILPPGGFLTLGLLLAAKQAWERHQAARASAAP
                     PLPMAPAAHCGG"
     gene            complement(12403..13041)
                     /gene="rsxG"
                     /locus_tag="PLH85_09335"
     CDS             complement(12403..13041)
                     /gene="rsxG"
                     /locus_tag="PLH85_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNB64806.1"
                     /translation="MDIKALQEKLGYHGVFLGLVGLLASAALATADMATHEDIRLAER
                     RDLEASLKQVLPQGFADNDLLKDTVTLARGDGKEVKVFRARLGGEAKGAVFEVSSRGY
                     AGDVVALLGVDTAGVVQGVRVLKHQETPGLGDKIEVAKSKWILDFDGKSFGTPPVEKW
                     GVKKDGGVFDQFAGATITPRAVVKAVREGMEFYRDHKDEIYSKPADGGGKEH"
     gene            complement(13055..14140)
                     /locus_tag="PLH85_09340"
     CDS             complement(13055..14140)
                     /locus_tag="PLH85_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNB64807.1"
                     /translation="MTQTTATVAAPVVAPHVAGSAGVARTMFLVVGALTPATLYGFWL
                     YGWPAIILFMLTLGSCVFFEYLCLHLAGAAPKRHVTDGSAILTGWLLAMTLPPWAPWW
                     TAVVGGFIAIVFGKQVFGGLGQNPFNPAMVARVALLVSFPVPLTLWVSPMPITAADAP
                     GFIDALRIVFGTLPIPDGVTSASLLGHVKTEMSRGVDLFHSLNGEHGHLLSYAGERAG
                     SLGETATWLILAGGAALLYLRVITWHIPAAMLLGVVVPAAIGHAVNPARFLDVTTHLM
                     SGGVMLGAFFIATDYVTSPSTTTGKLIFGFGCGFFTFVIRTWGGYPEGVAFSVLLMNT
                     LTPVIDRFTAPRILGRDRRGRPLQIKS"
     gene            complement(14137..15627)
                     /gene="rsxC"
                     /locus_tag="PLH85_09345"
     CDS             complement(14137..15627)
                     /gene="rsxC"
                     /locus_tag="PLH85_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNB64808.1"
                     /translation="MGFFDKIFSRHWGVHPEDHKRPAADAPVLKLPVAPRLFIPLSQH
                     VGAPARPQVVVGQRVLKGQCLAEAQGNISAPIHAPTSGTIVSIGEITAPHPSGLPLVA
                     ISIEPDGEDRWIDCKGLTDPFSESPQALAKRVAEAGVVGLGGATFPASVKLALGLRSK
                     IDTLIINGSECEPYLSCDDRLMRDKAADILSGVRVMLHSTGAQQALVGIEDNKPEAIA
                     AMKEAAAKYGEIRICPVPARYPMGSDRQLIVELTGREVPSDARAADVGVLVHNVGTAN
                     AVHQAIYEGRPLVSRLITLNGGAMAGPGNYEVLIGTLISDVIAFAGGYKGTPARLIMG
                     GPMMGTVLPHAAVPVVKGTAGVLAFTAAEVEEREARNCIRCGSCIKACPMGLMPLEMA
                     NFIRAEKLDGAEKIGLSDCISCGCCSYVCPSNIPLVQYFTYAKGAISAQTRAQLRQDA
                     TKKLAQARQERLDREAREKAEAAAKRKAERAAAQAAATKHTEGANA"
     gene            complement(15641..16171)
                     /locus_tag="PLH85_09350"
     CDS             complement(15641..16171)
                     /locus_tag="PLH85_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNB64809.1"
                     /translation="MIIAIASLTLLGLFLGLGLGVADRIFKVESNPFIAEIEAMMPGS
                     NCGQCGFPGCSGAAKALVEGHAPVTCCPPGGKALATALAARLGVSVDLSQVKDDGPRI
                     ALVSEELCIGCCRCSKVCPTDAIIGAAKQIHNVLREACTGCSSCVEKCPTEALSMKPV
                     PVTLQHWVWPKPAAAA"
     gene            complement(16194..16775)
                     /gene="rsxA"
                     /locus_tag="PLH85_09355"
     CDS             complement(16194..16775)
                     /gene="rsxA"
                     /locus_tag="PLH85_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNB64810.1"
                     /translation="MSELFLLLLTTALINNVVLVKFLGLCPFMGVTKKLDTAFGLGLA
                     TTFVITMASGACWMLENWILIPFDISYLRILMYILVIAAVVQFVEMVVKKMAPELYQA
                     LGVYLPLITTNCAVLGLPLISAQEKHTLFHAIMFGFGSSVGFTLVLAMFAGMRERLAL
                     ARIPGAFSGAPIGFVTASLLALAFMGFSGLNFN"
     gene            17078..18598
                     /gene="nifL"
                     /locus_tag="PLH85_09360"
     CDS             17078..18598
                     /gene="nifL"
                     /locus_tag="PLH85_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HNB64811.1"
                     /translation="MPALTRDKPQAILDALPSEAYRQAVDQADLAISITDPKARILFA
                     NEAFVRVTGYTPDEIIGQNESVLSNHTTPSSVYKEMWTELSAQRPWSGRLLNRRKDGA
                     LYLAELTISPVVDERGATTHFLGMHRDVTELHRLEKVVRNQKKLIESVVDSAPTAFAL
                     LDQSGRLILDNQEYKKLVADLRVKEPAHTLLDSVLANWREELAARPERCIFFNREARV
                     DRPGGRPRWLSVSATTLATQSDLADAYFSVGGQTGLLLVISDVSSVREEQERARTASL
                     QAVLAEEERTASLREGLSAAIFRLEEPMNVMASAVSMLQRRDPSAAAMLKQALTASRE
                     HMENLRQVIPHSPPEMVIPVNVNEILRDVLDICTPRLLSAGVVVDWSPAATLPPVIGR
                     PQQLRLLFKALMENAIEAMNVKGWKRRELSLSTAVERDCIVVSVIDTGPGIPAELRHK
                     AFEPFFTNKSSGRHLGTGLSRAQQIVSDHGGIIDLSPSPSGGCAAVVEFRLDGDPI"
     gene            18710..20344
                     /gene="nifA"
                     /locus_tag="PLH85_09365"
     CDS             18710..20344
                     /gene="nifA"
                     /locus_tag="PLH85_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HNB64812.1"
                     /translation="MQERPKLLDECPPVGGFCRTHELNIQLLAALYAVSRVLSRSLAF
                     GETLRDVLRVLDEEAGLGLGLIAVVDAQSGTLGTHVIHHPNVVGDVSYQPGEGVLGTI
                     LEKPRTIMLQRIADEPRFLNRLGIYQPGLPFIAVPIKAGSTLQGVLAVQPERPDDGLL
                     EERAQFVEMVANLIGQNLRLSLEVEQEKSSLVEERDQLRRTVRHQYGFDSMVGRSAVM
                     RRVFDQARMVAKWNTTVLIRGETGTGKELIANAIHYNSPRARNAFVRLNCAALPENLL
                     ESELFGHERGAFTGAVEARKGRFEQAHGGTLFLDEIGEISATFQAKMLRVLQEGEFER
                     VGGSRTIKVDVRIIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRERVEDILEI
                     ARHLLNKIGGEQKRKLALTDMANRRLASYDWPGNVRELENCLERAAVLSEDGRIDAEL
                     IRFPSARERSSPRPLRTLAPELDSPRASMPVDYAAPAAPLGNPLENPNLSEKERVVAA
                     LEQAGWVQAKAARILGMTPRQIAYRIQTLNIEVKQF"
     gene            20469..20798
                     /locus_tag="PLH85_09370"
     CDS             20469..20798
                     /locus_tag="PLH85_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNB64813.1"
                     /translation="MPRPKKHVLVCVQGRPPGHPRGSCQANGCAPVWQAFSDEFTTRN
                     LWASGFQLTNTGCLGPCNLGPTAIVYPEGTMYVGIKPEDVATIIDEHLMFDQPVERLK
                     APADVWE"
     gene            20803..21072
                     /locus_tag="PLH85_09375"
     CDS             20803..21072
                     /locus_tag="PLH85_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNB64814.1"
                     /translation="MSTVQYEIGDMVFAAEDLLNDGGMPGVTEEEGLIVAEGTRGVVV
                     HFGYAELDESKEIYLVRFENGPDGTLGAPVGCLPDELTQDVPVRN"
     gene            21077..21619
                     /locus_tag="PLH85_09380"
     CDS             21077..21619
                     /locus_tag="PLH85_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNB64815.1"
                     /translation="MPIGIFFGTDTGRTRLIAKQIAKKLGDAAAAPVNIGRTTLDDFL
                     AYDCWILGSPTLGDGELPGQSVGLSQPSWEEFLPQLDGVDLHGRTAAIFGLGDQKKYP
                     DEFVDAIGLLHDALLARGVRIVGRWPVAGYEFNQSKAQDGEHFLGLALDQHHQPVLTE
                     ARLDAWLAQIRAEMAAPVSA"
     gene            21645..22514
                     /gene="modD"
                     /locus_tag="PLH85_09385"
     CDS             21645..22514
                     /gene="modD"
                     /locus_tag="PLH85_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HNB64816.1"
                     /translation="MNPYTLPDTDLEALLQDDTPCGDATTHTLGIGSRRGRMVLRARY
                     AMTLCGSEEAARMGVLRGLNVDGPVLPSGSRLDTGTAFLSLSGPADALHAVWKTAQTL
                     VEYLSGIATCAAELLAAARRAHPDAGVVCTRKNFPGTKRAALKAILCAGASPHRISLS
                     ETLLIFPEHRAFLPPDESPAATLAHLRRQWPERPVVVEVAQAADALAWAAAGVDILQL
                     EKMSPDTVAALHAQISAGPGHPRLAAAGGITPANAEAYVRAGARILVTSAPYFAPPKD
                     VAVTLTPADEPRS"
     gene            22511..22903
                     /locus_tag="PLH85_09390"
     CDS             22511..22903
                     /locus_tag="PLH85_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64817.1"
                     /translation="MKVAIATQNFTIVSGHAGQSRQWLVYDLGEHHSSRLLPAPQRIE
                     LAEDEVMHVFKDDRPHPLDGVDVVVAASAGDGFVRHMRHRRADVLLTRESDPALAITH
                     ILAGEALPDPRFDVTTTLCKIRDLFSRY"
     gene            complement(23077..23331)
                     /locus_tag="PLH85_09395"
     CDS             complement(23077..23331)
                     /locus_tag="PLH85_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNB64818.1"
                     /translation="MRLIFSDAAWEDYLYWQHQDRKMVERINTLIQEVKREPFSGRGK
                     PEPLKHALSGFWSRRITDEHRMVYRIEGDAVLLAQLRYHY"
     gene            complement(23328..23579)
                     /locus_tag="PLH85_09400"
     CDS             complement(23328..23579)
                     /locus_tag="PLH85_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNB64819.1"
                     /translation="MDAITYSTARANLAKTMDRVCEDHAPLIITRNGEQSVVMLSLED
                     YNALEETAYLLRSPANARRLLSAVEQLAAGQGVERDVSL"
     gene            complement(23658..>24682)
                     /locus_tag="PLH85_09405"
     CDS             complement(23658..>24682)
                     /locus_tag="PLH85_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB64820.1"
                     /translation="SAVDMTLIKTEYLAQRNAVQGVSRRARLFGALPRWLHRYRGVLA
                     ALVRLRNASPWVAKKAEGWLGIAAQRSIPSITGHGVADRPGPVDAPRGRVALFVDTFT
                     HYYTPEVAEAAREVLEAGGYAVQVLRPAAGDDEPERPLCCGRSYLSQGLVDEAREEGR
                     RTMAALVPLLAEGVPVVGLEPSCLLALRDEFYSLSLGPEVGQLGKLAFLFEEFLMREG
                     NKGLKLPLKPLPGGRAVVHGHCHQKAFGAMKATRKVLGWIPGFEFDIVDASCCGMSGS
                     FGLEAEHYAASLKMGELSLLPAVRQAAPDALVIADGFSCRHQILDGTGREAEHVAVVL
                     RRALDV"
BASE COUNT         4270 a         8119 c         8158 g         4135 t
ORIGIN      
        1 tggaagacat ttgatagtct ctattgtttc ttttgtcgcc aaagaaacaa tagagcctat
       61 cgaagaagga atttgagggt gcggggaaga gtttgggcgg agcgggatgg aaatcggcgg
      121 ggcggggaaa gcgggcccgg tcacaattgt tccacgccat tctcgaaagc acacgcggcc
      181 gttcgccgca gagtgtcgag aacgtgccca ccaccacacc caccccgatt gaacacacgg
      241 gccaacatca tgctcgtctt cacctcggca ggccggagca atgcctgcgc ggctcgggtg
      301 tctttgccgg aatcgaacac agtccgacgc cccctcgcca tcgcacacca ggattgccct
      361 tcgcaacgtc atcacattcc ttatgcgcat gcgcaggctg gtcagaagcc gcaacccacc
      421 gccctacagc aagcatgcag gaaggccgcc ttccgcatcg tcgatccgac cctgcaagga
      481 caaaccctcg gtccaagtca atattgagag cgcgcgtctc ggattcttcg ccctcggcaa
      541 gccatccagc caggacaatt caaggtccgg tacctcccga tacagaccaa gcgaaaccga
      601 cagcgcctgg atgcgtgggg ccacattcac gtcgagatcc acctccaatg cccccggaaa
      661 cgtctcgacg ttccgccttc cagcatcccg cggttgccgc ttgtcatgaa agaagatgtg
      721 gcgtttaatg cccgactctc cggcggtcgt ttttgcccct gatccgcgcc tgaaccagac
      781 ccaacaagcg gggcgacaag ggagcagccg caagaagggt agacgtcatg atgacgcaaa
      841 cgccgcaaaa aattcttttt catacagaaa cttggtacat tccaacagct taggcgacag
      901 tcgtgtaaga cgccaggggt gtcagatgtc gtctacttca cgatcggcct caacctcgct
      961 atgaatcagc cgcagaagtt tgttgattta atcgaaaagt taattcgaga cgctcgcgtg
     1021 atccttgcgt ccgccagagg cggcttcatt gaagaccgcg aagcccttcg ccgctggtca
     1081 aatgagctaa ttcttcttcg taatctaggc ggaacgatgc tttcaccttg ggcacgtcga
     1141 ttggctcacg acggagtgct catacccgtc gagtacgtcc aacgcccgct ggccgcgctt
     1201 gagaccgtca aatatgcaac cgataacgga ctgctcactt cgtatcgaga cctcgttatc
     1261 gcggaggcat ttactgatct ctacgagcaa ggtcagtatt tgttcagcca gggttacttc
     1321 cttgctgctg gagtaatttt tcgcgcggtg ctggaagaaa gacttcgaga actttgtctt
     1381 gctgctgact gtatgccaga aaaggagcgt ccaactctcg gcgatctaaa tcaagctcta
     1441 ttccgctgtg aatcggtgcc atacgacaaa gcaatgatgc tacatgtcac ggcccttgct
     1501 gccgtcggaa acgatgccgc acacaacagc gattcgcttc aaaaagaaga tgttgagcgc
     1561 cttatgagag gcaccttgga attcctatcg agatactcgt ctacatgagg ccgaacctat
     1621 cattccaccg gaccttgcgc ataaagccgc gcaaggccgg tgaattcaaa cattgggcgt
     1681 ctctatgacc gcgcattctg ctcaagtcac ccaggagttc tgcaaacttt gcgactgggc
     1741 tttccaagtg tggctcaatc atcgatcaat gttcgatgac aatccgcggg ccgaagaact
     1801 tcaagaatca atggcggctg atgcactcgc gaggctgaga acgatcagtc acgagtactc
     1861 acttctccag atcgcgaaac ttcacgatcc ggtggtggtc gcgggccaag tgactctggg
     1921 catcgagtac atggtcaagt acggcgcgtg ggatgcggcc acttccgcaa cgcttagcca
     1981 gcttacggaa aagttgaata gcttcgccaa ggggcttcgt gcagttagaa acaaggcgct
     2041 ctctcacaac gaccttgccg ctgttcttag caatgcgaca ttaggcgagt tcgagaaaga
     2101 cgaagacatt cagtacttcg cgactcttca gcagtttgtc gatgtcgtac atggcgaggt
     2161 cataggtggc ccctggccct tcgatgatct tgcaaagaac gatgtggtag ccctcatggc
     2221 aatggtcaag tcatgaaaaa aattgccccc gacgcccaac ccattcgagc ggacctgcgc
     2281 gaaaagccgc gcaggccgct caattcaaac gttgtgcgtc atagagccat gagtccacgc
     2341 aaccgaaagc tgcttgccgc taccgctggt ctggtcttcg tattagcggg cgtcgtggtt
     2401 aagtttgccg ggcagggcgt cgttgcggcg ataggagata ccttcgttgc caccaattca
     2461 attcctggct ggtcagcgcc acaagaccgg tccagcttct atgctctctt cgtccttggg
     2521 ctgtgagtgt cggcgggtct ttccgtcaca gctttatcgc cctgcaagtt gcatggatct
     2581 caggtaagtc tttgcgacgt taggtgacta aacaatgagc aaccttgcat cgctctccgc
     2641 cttggctcaa gagtttcacg ccatggctgc cgggcttgac gtcgagccgt atttcaagtc
     2701 cttccacaca acgccgcaac acgatggctc acctcatatt gaggttgctg acgggaagtt
     2761 ccagtttgtt gtcactgagc gaggctctga attcgaaaga attaaaaacc tctcagctga
     2821 cgatgttctc tacctgctgt tcaacggaat tacccagcac atggctacga gatacgaact
     2881 caagaacaga gtccctgaaa ctgatggaag gtcagtttgg tttccgtatc aagagcgctt
     2941 gatggaacgc ctgaatcccg tttggggtga aaggctcagg aaggaacatc agcgcattct
     3001 cgaaaattat ccgttgcaac cgtgaaatca ccgccaccct atacaaaacg gccggatctc
     3061 ccgatccggc cgtcccgttt tccgtaaaac cccccgtcag gcgtaggtcg gcgcaaacgt
     3121 ctccgccggg ttggcggccg ggccgttttc gccccagtag ggggacagct tgcgcagctg
     3181 ggcgatgatc ggcggtacgg cggcgatgac gcggtcgatc tcggcttcgg tgttgtccac
     3241 cgacagggag aagcggatgg tgccgtgggc ggcggtgtag gggatgccca tggcgcgcat
     3301 gacgtgggag ggctccaggg agcccgaggt gcaggcggag ccggagctgc aggcgatgcc
     3361 cagcttgttg aggagcagca ggatggcttc gccttcgatg tattcgaagg cgatgttgct
     3421 ggtgttgggc aggcggttgt ccacgtcgcc ggtggggaag cagtgggaga tctggctcag
     3481 gatgccttgc tccagcttgt cccgcagggc cttgacctgg gtgttttcct tgtccaggtt
     3541 ggcgagggcc agttcgcagg cctggccgag gccgacgatg gaggcggagt tctcggtgcc
     3601 ggcgcggcgc ccgcgttcct ggtggccgcc ccgcagcagc gggcggaagc ggcagccgcg
     3661 gcgcaggtag agcacgccga tgcccttggg ggcgtggagc ttgtggccgg acagggagag
     3721 catgtcgatc ttgctgctct tgacgtcgat ggccaccttg cccacggcct gcacggcgtc
     3781 ggtgtggaac atgatgccct tggccgaggc gagttcggcc atctccacca cggggaagag
     3841 ggtgccggtc tcgttattgg cccacatcac cgagacaatg gccacgtcgt cgctgagcag
     3901 ggccttgtag gcttccagat cgaggcggcc cttgctatcc accgtgaggc ggtggacggt
     3961 gtagccctct ttctccagat gttcgcagag ggtgaggatg gccgggtgct cgacgacggt
     4021 ggtgatcacc gtcttgcggt ccggctgggc cttgagggcg gagaggatgg cggtggagtc
     4081 ggactcggtg ccgcacgagg tgaagatgat ctccgaatcg tgctcggcgc cgatgagggc
     4141 ctgcacctgg ccccgtgcct tcttgagggc ggcgcccact ttgctgccga aggcatggat
     4201 ggacgaggcg ttgccgaagt gctcggtgaa gaagggcagc atcgcgtcca ccacggcggg
     4261 gtcgacccgc gtggtggcgt tgttgtccag gtagatcggt tccatggcgc agtccttgtc
     4321 agattctctg tggggggatg tcgggatgtc aggcttcgac gggcatgtgg ctggccggca
     4381 ggacctgcac cagttcgccc agggcctcga tcatcttctg ctggatgccg ccgagggtcg
     4441 ccgcttccat catgcagccg gagcaggcgc ccttgaggtg cacgtagatc ttcttgccct
     4501 gcacgtcggc cagttccacg tcgccgtggt cgcgctggag catggggcgg acggattcga
     4561 ggacttcctc gatcttcttg atcttctgca ccagggtgag cttggcggca ggcggcttgg
     4621 gcgcttccgg cgtggccggg caggcgggcg ggggcgacac ttcgccggtg ccgggcagct
     4681 cggccttgac gcgggcgagg atctcctcga tgccttcgtg gcaggcggcg cagccaccgc
     4741 cggccttggt gtagaaggtg acttcctcga cggtgttgag gttgttggcc ttgatcacgt
     4801 cttcgatcat caccgcatcc acggcgaagc acttgcagat gagggcgcct tcttcgtggt
     4861 cgtcggacca ttcctcgccg cggtagttgg cgatggcggc ctgcagggct tcgcggccca
     4921 tcacggagca gtgcatcttt tcggggggca ggccgtccag gtagtcggcg atgtcctggt
     4981 tggagacctt gagggcttca tcgagggtca tgcccttgat gatctcggtc agggccgacg
     5041 aggaggcgat ggcggagccg cagccgaagg tctggaagtg cgcgtcctgg atgacttcgg
     5101 tttcgggttc gaccttgagc atcaggcgca gggcgtcgcc gcactggatg gagcccacgt
     5161 cgccgatgcc gttggcttct tccagggggc cggagttgcg cgggttgaag aagtgttcgc
     5221 ggaccttgtc agagtattcc cacatggttg gcgctcctta gaccgagaag gaagaaccgc
     5281 aggcgcactg ggcgctggcg ttggggttgt cgaacttgaa accggtgtgg gtcagggtgt
     5341 cgacaaagtc gatggtgacg ttgtccacca tcgggaaggt catcgggtcc accaggacct
     5401 tgacaccttc gatgtccagt tcccaatcgt cctcggcctt ggcggcttcg agcttcatgc
     5461 cgtactggag gccggagcag ccaccgcccg agaccaccag gcgcaggccg gcgacggggg
     5521 tttcggagcc acggatgaag cgttggacgg ctttcactgc tgcgggggtc agattgatca
     5581 tgttggttct cccagttagg atgaagagaa cctcgcaagc cctgtgccat cttctgcatc
     5641 gcggtaagcg attgaattga ctggcttgtt tcaaagaccg ccgttgtcgt atttacgaca
     5701 atcagcccag cacgtgttcg taggcgcggc gcaggagctg gcgctccaca tcgctgaggt
     5761 gacgtgattc ggtgagggcg atgaagcgcc cctggctgcg cgcggccacc tcgaagggcg
     5821 gctcgatgcc catgaagagg cccatcagca cgggcacatc gccggcaggc gtatccaccc
     5881 aggcctggaa atccgcctcg gtctggatgg tgccttcggc catgcccaga tgcacctcgg
     5941 cctcgcgcac caccgcggtg ggatgcacct gcaccttggg gctgtaggcc tcgtcgcgca
     6001 gggctgccag gggcggcagg ggcgcaaagg cgagcacgcc cgaggcgagg cacaagaagc
     6061 gcacccgccc gctggtgccg tgcttgtgca gcaggatcag gcgcggcgaa ggcaaggccg
     6121 acacgcggag ctcacttggc aaactcggcg atcaccgcgt ctccccgcag acgttcggcg
     6181 ctgccccaca cctggacgga ctccaggtag ctgtcggcct tgtccggcag gggcagcagc
     6241 acctggtact cggcaaagat gcgcccgtcg gcaaagaagg tgacgttgcc gtagcgcagc
     6301 ccgccgctcc aggtgccgac ccggctctct tcctgagaga aaggatcggt gcgcgtctcg
     6361 aagacggcgt cggcccacac cggctcgtcg ccgatggaca ggcccagctt ggccacctgg
     6421 gcgcgcaggg cctcgcagat gcgggccccc agcgccggat cggggaccgg cagggccatc
     6481 acacggacgc cggcgcgtag gtgtagcagt ccttgggaca gacgcgcccg caggagccgc
     6541 agccgatgca gtccatggcg ttggcgatgg acatgaccat catgttgtcg tcttcggagt
     6601 cgtcgtcatc gtcgagctcg cgctcgacca ggtcgagcac gttgcgcgga cagaccttgt
     6661 agcagcggcc gcagccgatg cacttggcct ggttgagctc ggtgacgaac tggggcgtcc
     6721 attcggcgcc gccgcgggtg agaccggtga tctggctcat ggtgatcagc ctttctgggc
     6781 ggcggcaagg cgggcgttgg cgtcggccca ggccttgcag gcctcgtaag tggattggga
     6841 cagggtggga atctcctggt aggcggccgg caggcggtct tccaccagat cgtgcatctg
     6901 cgaggcccac tcggaagcga tgcgcttgag cttcttcact tccttgtcga gggcctgcag
     6961 gtcttcgggg gtgaggacgt cggacatgat ggctcccgga ctcaggcgtc ggcgacgtcg
     7021 cggtgttcgg tgatgcgggc cacggcgccc ttcaggatct tgtcgccctc ttccttcatc
     7081 ttctccaggc tggcgaagcc gaagcgatgg acgtcgcgca gggtcttgtc caccgccacc
     7141 agacggccca cggtgatgag ggcgcggccg aagccttcgt gggtgaggtg gatcaggggc
     7201 acggcgagga ggccggcttc cttctcgatg agcacggcga tcgagttgta gaaggccttc
     7261 acgcgggaga tggtttcgtc gtccgggtcg ccgatgaggg ggatctcggc cttgcgttcc
     7321 ttggtgagga cgtagggcgc gaggatcttc tcgacgctcc agccggtgta ggtgccgtag
     7381 gtgtcgaggg cacgcatctg gcgggccatc tccttgacga agtccgtctc gaggatggga
     7441 tcagcggtcg tggcggtcat gatgaaactc cagaggggaa agggtggagg ccgcgggggc
     7501 cagaggaatg ggggacagct ggggcgtcgg catcaggtcg ggcatcaggc tgatgaggat
     7561 gcgcaggatc agcatggcgc ccaggaagcc gccgatggcg ccgagggcgg cagccgcgtc
     7621 gctgccctcc acggccatgc cgagggcggc gccgagcatc atcacgaagg ccgggaagag
     7681 gtagcccagc atggcgaaga gggtcatgcc ccgctcgggc agggtgacct ggacggcatc
     7741 gccggccttg tagtcggcgt cggcgcgcac ggtcagcagg gtgctgtcct ggccctgggc
     7801 caggcggccc acgccgcagc cggcgccgtg gtcgcaggag gcgcagccgg aggtttccat
     7861 ggcgacgaag accttgccgc cggcgacacg gcgtaccacg ccgcggtgtt cgatgtgtgc
     7921 gctgctcatc cctgccagcc ctcctcttcc atcgagttga aacggtccgt cgaggcggcc
     7981 ttggcctgca gggccagggc ccggtcaatc cagggcacgc cgccttcagt catggccttg
     8041 ccgatgtcgg cgagcaggtc gaacacggcg tccacctcgt tggtgcggat gggctggatg
     8101 cccttggcga gaagctgctt gatcgccgag gcgccgatgg ccatcacgta gaccgcggcg
     8161 cagccttcca ggaagttcac cttggcggcg agcttgcctt cgttgccgtc catggcctct
     8221 tcggggaact cggccacgcc gacgagggtg gccttgtcgg gcgtgacctc gtagagggcg
     8281 aaggactcgg cagcgccgaa gtgctggtcg acccggacgc ggtcggacgt ggcaaaagcg
     8341 accttgagca cggcagcggg ctccttttta atggaccaga ccagttgcag ccgacggggt
     8401 gtcatggtgt tcgcaggagc cgccggcgtc ccgggactcg ttccaataca cggatcgata
     8461 gggttcgagt tcatggtgtt ggctcagcat caggttggcg agatcgaaca gtgcctgccg
     8521 cgagccccgg tagccgaccc acgttcgggc gtagccgccc accaggtcgt actgcggaaa
     8581 gcccgcgcgc atcagcggca gaccgagacg ccgcgcggtc tcggcggcgt gggaattggc
     8641 aatgacgagc tgggcgccgc gcttgcgggc ctgcagctcc aggtcttcca ggtcgcccac
     8701 cgtcacctgc tcgatgggca gttcggcgag gctgtcggcc ttggccgggc tcaccgcggc
     8761 gacgatctcg gcgcccatgc cggtgaggaa gtgggcctgc atgcccagca ggtccgggtc
     8821 ggcggccagc gcgacgcggg caaagccaag catgaagtgg caatccacca tggcgtcctg
     8881 caactgggcg cgctggcggt cgatgcgttc gggcaccgga cggccggaga tctcggcgag
     8941 ggcctgggtg agggcgtcgc aggcatccag gcccatcagg ccggcaaagc ggaagtcggg
     9001 cacgccggtg cgggccttga gcttgtcggc ggcgcggtag agggacgggc cgatcaccag
     9061 cgtggcgacg gattcgccca tgctttcgat ctcctgccgc ggcgtgccac cgagggtgag
     9121 ggacgagtac tccgcctcca ccaggtgacc gtcgagggag tcgccgatgt ccggcaccac
     9181 caccgggcgc aggccgaagg cttccaccca ttccttgatg gcctcgatgt cgcccggcgt
     9241 gagcatgccc gaggcgagca cattcacctg acgcggccgg cgtccggcac agctgctttc
     9301 cggcaccagg gtctcgatga gggactcgat ggccagcgcg tagccgcttt ccaggcaacc
     9361 cacgtagtcc ggcgtgctca cgggcaccac ggccacgtgg gcgaactccg gatgggcctc
     9421 gcgaaagttg cgcaccgcgc ggcggatgtc ggtgccctgg gtctccgaga ggccggtggt
     9481 gaccagaccg atcaggtccg gcttgctctt gtcggagagg gtacgcaggg cttcgaccac
     9541 gttgtcgtcg gcgcccatga tggtggagac ctggtccatc gccgtggtct gcagcgggat
     9601 gggctcgcgg aagtggcgca cgaagaagac cttgccgaag gcggtgcagc cctgggagcc
     9661 gtgcatcagg ggcaggctgc gggcgatccc cagaaaggcc agggacgcgc cgacaggctg
     9721 gctgaccttg aggggattga ccgcgagggc cttcttggaa tggacgatct ctgccatggt
     9781 cgtgtcctca ggccgccagc acggtgccgg gaccgctctg cttggcccac ggggccgggc
     9841 ggcgcaccgc ctcccacacc gggctgttca tggacagggc gagctggcgg gccagctcca
     9901 ccatgccgtc gtaaccggcg tagccgaatt cccgctcctg gttgatgtcg aggaagggaa
     9961 tccgcgcctt gagggccgtg tagaggttgc ggccgccggc gatgaggatg tccgccttgt
    10021 attccttgta gatggacagc agcgccttgg ggctgccgtc gtcgatcatc ttggtgtcgt
    10081 cgcccatcag ctcgcggatg cgcgccttgt cttcctcggt ggatttcttg gtgccggtgg
    10141 ccaccacctt catgcccaga tcctgcaggg ccgagacgat ggaccaggac ttgacgccgc
    10201 cggtgtagag cagcacgcgc ttgcctgtga ggcgttcgcg ccacggatcc aggtcggcgc
    10261 gggacttggc ctcctcgcgg gcgatgacgg cctcggtgcg ggcgatcagg tccgggtcgc
    10321 cgatgatgcg ggcgaagtcc cgcagcgcgg tggacatgtc ctgcacgccg tagaagctgc
    10381 cttcgaagaa gggaatgccg taggtgtcct gcatcttgcg ggccacgttg agcagcgcct
    10441 tggcgcacac caccatattc accttggcgc ggtgcatggt ctgcacctcg tggaagcggg
    10501 cgtcgcccga cagggtgcac aggatgcgca ggcccagctc gtcaaagagg ggcgagacat
    10561 gccagaactc gccggcaatg ttgtattcgc cgatcaggtt cacgtcgtag gtgggacggc
    10621 cgtccacccg gggcgcggcg ggctccggtt cgcgggtgcc gatcacgtgc ttgaacatgg
    10681 cttcgccggc aaggcggttg cccaggttct tggtgccgta gaaaccggcc gcatccacgg
    10741 gcacgaccgg ggtcccccac cgctcggtcg ccgccttgca cacggcctcc acgtcgtcgc
    10801 cgatgagggc ggtgacgcag gtgttgtaga cgaacaccgc agccggcgag taggtctcga
    10861 cggcctgctt gatggcgtgg aacagccgct tctcgccgcg ccccatcacc acgtcggtct
    10921 cggagagatc ggtggtcatg ccgatgcggt acagcgtcgc ccccgacgag cgcgtgcccc
    10981 ggttgtccca ggacgaaccc gcgcaggcga tggggccatg cacgatgtgg gcaacgtcgg
    11041 cgatgggcag gagcgcaatc tgcgccccgt cgaaggagca gccgcccgcg gtggcgccgg
    11101 gcttgggccg ggcacagccg gacttttcct tcttgttgtg ggtgcaggcc ggctcgtcga
    11161 gcagagcctg gatttcgctg gctttcatgc tggtctctcc agatgtccat cctacgaaca
    11221 gcaagatgga tgccaaacac aagcccctga aagcacggca gtttttgcag cgcggcgatg
    11281 tcgcaaaccc gacaaacccg ccttgtacga cacgccgcgg agggatctgc gacaggccag
    11341 gtcgccgctc acgcccgacg cgcccgtgcc ggcaataaaa aaaccccggc cggagccggg
    11401 gttctgtgca agtgccaacc tgcagtcatt cttcgtcctg atcccgccgc ggtacggttt
    11461 ccgggtcggc gatgatgccg cggtagatct ccacccggtc gccgggcttg agggcggcgt
    11521 tgagtttgac cagccgcccg aacacgccca ccttctgggc cgccaggtcg atgtggggga
    11581 actgcttgag gatgccggag cgctggatcg cgtcctcgac cgtggattcg tcgggcacct
    11641 cgatgttgag ccagatctgc tggccaggtt cggaatacgc aacgccgatc tgcatggtcg
    11701 tctcctcctc aaccgccgca gtgggccgcc ggggccatgg gcaggggcgg cgcggccgag
    11761 gcacgggcgg cctggtgacg ttcccacgcc tgcttggcgg ccaggagcag ccccagggtg
    11821 aggaagccgc cggggggcag gatcatcagc agcgtgccgc cgtagcccgg cagggcattg
    11881 atctccagga aggagaagga cgggccgagg agcaggctgg cttgcgagaa cagggtgccc
    11941 tggccaacga attcccggca caggccgatg gtggtgaggg cgaaggtgaa gcccaggccc
    12001 atccacaggc cgtccatggc cgactggagc accggcttgc gcgcggcaaa ggcttcggcg
    12061 cggccgagca cggcgcagtt ggccacgatc agcgcgatgt acatgcccag caccttgtag
    12121 aggtcgtgca tccaggcgtt gatgcccagg tccaccaggg tcaccaggca ggcgatcagc
    12181 acgatgtaca ccgggatccg cacctgcggc gtggtcacgt tgcgcacgct ggacaccagc
    12241 gcgttggaga ccagcagcac ggcggtggtc gccagcccca tgccgaggcc gttggtgccg
    12301 ctggtggtca ccgccatggt ggggcacatg cccaggatct gggagaagac gatgttctgc
    12361 ttccacagcc catcggtgca gatgtcgcgg tactcgctca tgtcaatgct cctttccgcc
    12421 gccgtcggcc ggtttggaat agatttcgtc cttgtggtcg cggtagaact ccatgccctc
    12481 ccgcactgcc ttgaccaccg cccgcggcgt gatggtcgcg ccggcaaact ggtcgaagac
    12541 gccgccgtcc ttcttcacgc cccacttctc gaccggcggc gtaccgaagg acttgccatc
    12601 gaagtccagg atccacttgc tcttggccac ttcgatcttg tcacccaggc ccggcgtctc
    12661 ctggtgcttg aggacgcgca cgccctgcac cacgccggcc gtatccacac cgaggagcgc
    12721 caccacgtca ccggcgtagc cgcgggagct cacctcgaag acggcgccct tggcctcgcc
    12781 gcccagacgg gcgcggaaga ccttcacctc cttgccatcg ccgcgggcga gggtcacggt
    12841 gtccttgagc aggtcgttgt cggcaaaacc ttgcggcagc acctgcttga gggaggcttc
    12901 cagatcgcgc cgctccgcca gacggatgtc ctcgtgggtg gccatgtcgg cggtggcgag
    12961 ggcggcgctg gcgagcaggc cgacgagacc gaggaacacc ccgtgatagc cgagcttctc
    13021 ctgtagggcc ttgatatcca tgctgttctc cgggtcagga cttgatctgc agcgggcggc
    13081 cgcggcggtc gcgtcccagg atgcggggcg cggtgaaacg gtcgatgacc ggcgtcaggg
    13141 tgttcatcag cagcaccgag aaggccaccc cttccgggta gccgccccag gtgcggatga
    13201 cgaaggtgaa gaagccacag ccgaaaccga agatcagctt gcccgtcgtc gtggaaggcg
    13261 aggtcacgta gtcggtggcg atgaagaagg cgccgagcat cacgccgccg gacatcaggt
    13321 gggtggtcac gtccaggaaa cgcgccggat tgaccgcgtg gccgatggcc gccggcacca
    13381 ccacgccgag gagcatggcc gccgggatgt gccaggtgat cacccgcagg tagagcagcg
    13441 ctgcaccgcc cgccaggatc agccaggtgg cggtttcgcc caggctgccc gcgcgctcgc
    13501 cggcgtagga cagcaggtgg ccatgttcac cgttaaggga gtggaacaga tcgacgccgc
    13561 gggacatctc ggtcttcacg tggcccagca gggaggcgct ggtcacaccg tcggggatcg
    13621 gcagggtgcc gaaaacgatg cgcagggcgt cgatgaagcc cggtgcgtca gccgccgtga
    13681 tcggcatggg cgacacccac agggtgaggg gcaccgggaa ggacaccagc aaggccaccc
    13741 gggcgaccat cgccggattg aaggggttct ggccgaggcc gccaaacacc tgcttgccga
    13801 agacgatggc gatgaagccg cccaccaccg cggtccacca gggcgcccag ggcggcaggg
    13861 tcatggccag cagccagccc gtgaggatgg ccgagccgtc ggtgacgtgg cgcttcggcg
    13921 ccgcgccggc cagatgcagg cacaggtact cgaagaacac gcaggagccg agggtcagca
    13981 tgaacaggat gatcgccggc cagccgtaca gccagaagcc gtacagggtc gccggcgtca
    14041 gggcgccgac caccaggaac atggtgcggg cgacacccgc gctcccggcg acgtgggggg
    14101 cgacgacggg cgccgccaca gtggctgtgg tctgggtcat gcgttggctc cctcggtgtg
    14161 cttggtggcg gcggcctggg ccgcagcccg ttcggccttg cgcttggcgg cggcttcggc
    14221 cttttcacgg gcttcccgat ccagacgctc ctgacgggcc tgggccagct tcttggtggc
    14281 gtcctggcgc agctgggcgc gggtctgggc ggagatggcg cccttggcgt aggtgaagta
    14341 ctggaccagc gggatgttgg acggacacac gtaagagcag cagccgcagg agatgcagtc
    14401 ggacaggccg atcttctcgg ccccgtcgag cttttcggcg cggatgaagt tggccatctc
    14461 caggggcatc aggcccatcg ggcaggcctt gatgcaggag ccgcagcgga tgcaattgcg
    14521 cgcttcgcgc tcttccacct cggccgcagt gaaggccagc acgcccgcgg tgcccttgac
    14581 caccggcacc gccgcgtggg gcagcacggt gcccatcatc gggccgccca tgatcaggcg
    14641 tgccggggtg cccttgtaac cgccggcgaa ggcgatcacg tcggagatca gggtgccgat
    14701 cagcacctcg tagttgccgg ggccggccat ggcgccgccg ttcagggtga tcaggcgcga
    14761 caccagcggg cggccttcgt agatcgcctg atgcacggcg ttggcggtgc ccacgttgtg
    14821 caccagcacg cccacgtcgg cggcgcgggc gtcggagggc acctcgcggc cggtcaattc
    14881 cacgatcagt tggcggtcgg aacccatcgg gtagcgcgcc ggcaccgggc agatgcggat
    14941 ctcgccgtac ttggcggccg cttccttcat ggccgcgatg gcttcgggct tgttgtcctc
    15001 gatgcccacc agagcctgct gggcgccggt ggagtggagc atcacgcgca cgccgctgag
    15061 gatgtcggcg gccttgtcgc gcatcaggcg gtcatcgcag gagagatagg gctcgcactc
    15121 gctgccgttg atgatcaggg tgtcgatctt ggagcgcagg cccagagcca gcttgaccga
    15181 ggccgggaag gtggcgccgc cgaggcccac cacgccggct tccgccacgc gcttggcgag
    15241 ggcctggggc gactccgaga aggggtcggt gaggcccttg cagtcgatcc agcggtcctc
    15301 gccgtccggc tcgatggaga tcgccaccag gggcagaccg gagggatgcg gcgcggtgat
    15361 ctcgccgatg gagacgatgg tgcccgaggt gggtgcgtgg atcggcgccg agatgttgcc
    15421 ttgcgcctcg gccaggcact ggcccttgag cacccgctgg cccaccacca cctgcggacg
    15481 cgccggggcg cccacgtgct gggacagggg aatgaacagg cgcggcgcga cgggcagctt
    15541 cagcaccggg gcatcggcgg cgggacgctt gtggtcttcc ggatggacgc cccaatggcg
    15601 cgagaagatc ttgtcaaaaa aacccatgat cgatggctcc tcaggcagcg gcggccggct
    15661 tgggccagac ccagtgctgc agggtgacgg ggacgggctt catgctcagc gcttcggtcg
    15721 ggcacttctc gacgcagctg ctgcagccgg tacaggcttc ccgcaggacg ttgtggatct
    15781 gtttggcggc gccgatgatg gcgtcggtcg ggcacacctt gctgcagcgg cagcagccga
    15841 tgcacagctc ctcggacacc agggcgatgc gcggaccgtc gtccttcacc tgggacagat
    15901 cgacggacac gcccagacgc gccgcgaggg cggtggcgag ggccttgccg cccggcgggc
    15961 agcaggtgac gggggcgtga ccttcgacga gggccttggc ggcgccggag cagccgggaa
    16021 agccgcactg gccgcagttg gaacccggca tcatcgcctc gatctcggcg atgaaggggt
    16081 tgctctccac cttgaagatc cggtcggcga cccccagccc caggcccagg aacaggccga
    16141 ggagggtcag gctcgcaatc gcaatgatca ttggggttct cctgtcgcgg gttttagttg
    16201 aagttcaggc ccgagaagcc catgaaggcc agggccagca ggctcgcggt gacaaaaccg
    16261 atgggcgcgc ccgagaaggc gccggggatg cgggccaggg cgaggcgttc gcgcatgccg
    16321 gcgaacatgg ccagcaccag ggtgaaaccc accgaggagc cgaagccgaa catgatggcg
    16381 tggaacagcg tgtgcttttc ctgggcgctg atgaggggca ggccgagcac cgcgcagttg
    16441 gtggtgatca ggggcagata cacgccgagg gcctggtaga gctcgggcgc catcttcttc
    16501 accaccatct cgacgaactg caccaccgcg gcgatcacca ggatgtacat caggatgcgc
    16561 aggtaggaga tgtcgaaggg gatcaggatc cagttctcga gcatccagca ggcgccgctg
    16621 gccatggtga tcacgaaggt ggtggcgagc ccgaggccga aggcggtgtc gagctttttg
    16681 gtcacgccca tgaagggaca caggccgagg aatttgacga gcaccacgtt gttgatcagc
    16741 gcggtcgtca gcaggagcag gaacaattcg gacatggcgg catctcgaat gaggggattg
    16801 atgccctcaa ggcacaagcc gtgccagcct tttgccgcac tgcaaaaacc cttcaattcc
    16861 gggggttttc gtgtgcgggc accacgccgg tgcgcgccgc acccgaaaaa gtgtcgcaaa
    16921 accgacagcc ctttcgtcgc acttgcccca gaaaccaccg cgccgcaaca aaaccacggc
    16981 cagacaggga tttagggctt tcggcatgga acatgcttga aatttccggc gttacacgcc
    17041 cccgtccgtc accgcgattc cgtctcagga gccccccatg ccggccctca cccgcgacaa
    17101 accccaggcg atcctcgatg cgctgccctc cgaagcctat cgccaggcgg tggaccaggc
    17161 ggatctggcc atctccatca cggaccccaa ggccaggatc ctcttcgcca acgaggcctt
    17221 cgtgcgggtc accggctaca cgccggacga gatcatcggc cagaacgaat cggtgctgtc
    17281 caaccacacc accccgtcgt cggtttacaa ggagatgtgg accgagctgt ccgcccagcg
    17341 cccctggtcc ggccggctcc tcaaccggcg caaggacggc gccctgtatc tggcagagct
    17401 gaccatctcg ccagtggtgg acgaacgggg cgccacgacc cacttcctcg gcatgcaccg
    17461 ggacgtgacc gagctgcatc gcctcgaaaa ggtcgtgcgc aaccagaaga agctcatcga
    17521 atcggtggtg gattccgcgc ccaccgcctt cgccctgctg gaccagagcg gccgcctgat
    17581 cctcgacaat caggaataca agaagctggt cgccgacctg cgcgtcaagg agcccgccca
    17641 caccctgctc gacagcgtgc tcgccaactg gcgcgaggaa ctcgccgccc gccccgagcg
    17701 ctgcatcttc ttcaaccggg aagcccgcgt cgaccggccc ggcggccggc cgcgctggct
    17761 gtcggtgagc gcgaccaccc tcgccaccca gagcgatctg gcggacgcct atttcagcgt
    17821 cggcggtcag accggcctgc tgctggtcat ctccgacgtg agcagcgtgc gcgaggaaca
    17881 ggaacgcgcc cgcaccgcct ccctgcaggc cgtgctggcc gaggaagagc gcaccgcgtc
    17941 cctgcgggaa ggcctctccg ccgccatctt ccgcctcgaa gaacccatga acgtgatggc
    18001 ctcggccgtc tccatgctgc aacggcgcga tccgtccgcc gccgccatgc tcaaacaggc
    18061 cctcaccgcc agccgcgagc acatggagaa cctgcgccag gtgatccccc acagcccgcc
    18121 tgagatggtc atcccggtca acgtcaatga gatcctgcgg gacgtgctgg acatctgcac
    18181 gccgcgcctg ctctcggccg gcgtggtggt ggactggtca ccggcggcca ccctgccccc
    18241 ggtcatcggg cggccgcagc aactgcgcct gctcttcaag gccctgatgg agaatgccat
    18301 cgaggcgatg aacgtgaagg gctggaagcg ccgcgagctg agcctgtcca ccgccgtgga
    18361 gcgggactgc atcgtcgtct cggtcatcga caccggcccc ggcatcccgg ccgagctgcg
    18421 ccacaaggcc ttcgaaccct tcttcaccaa caagagcagc ggccgccacc tgggcaccgg
    18481 cctgtcccgc gcccagcaga tcgtgtcgga ccacggcggg atcatcgacc tgagccccag
    18541 ccccagcggc ggctgcgcgg ccgtcgtgga attccgcctg gacggcgacc cgatctaagc
    18601 ccgccgtccc gcaccgggcg ccgcccgcgg cgccggctag aagactttcg tccgccaccg
    18661 cctccaggcg cggcggccct gaaggacata acctgtgagc gcccacggca tgcaagaacg
    18721 cccgaaactc ctcgacgaat gcccccctgt gggcggtttc tgtcgcaccc acgagctgaa
    18781 catccagctc ctggcggccc tctacgccgt cagccgggtc ctcagccgct ccctggcctt
    18841 cggcgagacc ctgcgcgacg tcctgcgcgt cctcgacgag gaggcgggcc tcggcctggg
    18901 cctcatcgcc gtcgtggatg cccagtccgg caccctcggc acccacgtga tccatcaccc
    18961 caacgtcgtc ggcgacgtct cctaccagcc cggcgagggc gtactcggga ccatcctcga
    19021 gaagccgcgc accatcatgc tgcaacgcat cgccgacgaa ccgcgcttcc tcaaccgcct
    19081 cggcatctac cagcccggcc tgcccttcat cgccgtgccc atcaaggccg gcagcaccct
    19141 gcagggcgtc ctggcggtgc aaccggaacg cccggacgac ggcctgctgg aggagcgcgc
    19201 ccagttcgtg gagatggtgg ccaacctcat cggccagaac ctgcgcctgt ccctcgaagt
    19261 cgagcaggag aaatcctccc tcgtcgaaga gcgcgaccag ctgcgccgca ccgtgcgcca
    19321 ccagtacggc tttgacagca tggtggggcg ctcggcggtg atgcgccggg tcttcgacca
    19381 ggcccgcatg gtggccaaat ggaacaccac cgtgctgatc cgcggcgaga ccggcaccgg
    19441 caaggagctc atcgccaacg ccatccacta caactcgccc cgcgcccgca acgccttcgt
    19501 gcgtctcaac tgtgcggccc tgcccgaaaa cctcctcgaa tccgaactct tcggccacga
    19561 gcggggtgcc ttcaccggcg ccgtcgaggc gcgcaagggc cgcttcgagc aggcccacgg
    19621 cggcaccctg ttcctcgacg aaatcggcga gatctccgcc accttccagg ccaagatgct
    19681 gcgtgtgctc caggaaggcg agttcgagcg ggtcggcggc agccgcacca tcaaggtgga
    19741 tgtgcgcatc atcgcggcca cccaccggga cctggaaacc gccgtggaga tgggcgactt
    19801 ccgcgaggat ctgttctacc gcctcaacgt gatgcccctc ttcctgcccc cgctgcggga
    19861 gcgtgtcgaa gacatcctgg agatcgcccg ccacctcctc aacaagatcg gcggcgagca
    19921 gaagcgcaaa ctggccctca ccgacatggc caaccgccgc ctcgccagct acgactggcc
    19981 gggcaacgtg cgcgagctgg aaaactgtct ggagcgggcc gccgtgctct ccgaggatgg
    20041 ccgcatcgac gccgagctga tccgctttcc cagcgcccgc gagcgcagct cgccgcgccc
    20101 cctgcgcacc ctcgccccgg aactggacag cccgcgcgcc agcatgccgg tggactacgc
    20161 cgcgccggcc gcaccgctcg gcaatcccct cgagaatccc aatctctccg agaaggagcg
    20221 cgtcgtggcg gccctcgaac aagccggctg ggtgcaggcc aaggccgccc gcattctggg
    20281 catgaccccg cgccagatcg cctaccgcat ccagacgctc aatatcgagg tcaagcagtt
    20341 ctgaccgggc cgccccccgg attgtcgcaa acccgacatc ccacacaggc cccaagccta
    20401 tgaatttcaa gtgatttaat ctggcatcgg gcttgcaagg aagtgctcac ccaacccgga
    20461 gagacaccat gccccgcccc aagaaacacg tcctcgtctg cgtccagggc cgcccgcccg
    20521 gccatccccg cgggtcctgt caggccaacg gctgcgcccc cgtgtggcag gccttctcgg
    20581 atgaattcac cacccgcaac ctgtgggctt cgggcttcca gctcaccaac accggctgcc
    20641 tgggcccctg caacctgggc cccacggcca tcgtctatcc ggaaggcacg atgtacgtgg
    20701 gcatcaaacc ggaggacgtg gccaccatca tcgacgagca cctgatgttc gatcagccgg
    20761 tggagcgcct caaggccccc gccgacgtgt gggagtgaga ccgtgagcac cgtccagtac
    20821 gagatcggcg atatggtctt cgccgcagaa gacctcctca acgacggcgg catgcccggc
    20881 gtcaccgagg aggaaggcct catcgtcgcc gagggcaccc gcggcgtggt ggtccatttc
    20941 ggctacgccg aactcgacga gagcaaggag atctacctcg tgcgcttcga gaacggcccc
    21001 gacggcaccc tcggcgcccc ggtgggctgc ctgcccgacg aactcaccca ggatgtcccc
    21061 gtccggaact gacaccatgc ccatcggcat cttcttcggc accgacaccg gccggacccg
    21121 cctcatcgcc aagcagatcg cgaaaaagct cggcgacgcg gcagccgcgc cggtcaacat
    21181 cgggcgcacc acgcttgacg acttcctcgc ctacgactgc tggatcctcg gctcgcccac
    21241 cctgggcgac ggcgaactgc ccggccagtc cgtgggcctc agccagccca gctgggaaga
    21301 gttcctgccc cagctcgacg gcgtggatct gcacggccgc accgccgcga tcttcggcct
    21361 gggcgaccaa aagaaatatc cggacgagtt cgtggacgcc atcggcctgc tccacgacgc
    21421 cctgctcgcc cgcggcgtgc gcatcgtggg gcgctggccc gtggccggtt atgaattcaa
    21481 ccagtccaag gcccaggatg gcgagcactt cctcggcctc gccctcgacc agcaccacca
    21541 gcctgtcctg accgaggccc gcctcgacgc ctggctcgcc cagatccgcg ccgagatggc
    21601 cgccccggtg agcgcctgac cgggtttcac gtccacggag gccgatgaac ccctacaccc
    21661 tgcccgacac cgacctggaa gccctgctcc aggacgacac cccctgcggc gacgccacca
    21721 cccacacgct gggcatcggc agccgacggg ggcgcatggt cctgcgcgca cgctacgcca
    21781 tgaccctgtg cggcagcgag gaagccgccc gcatgggcgt acttcgcggc ctcaacgtcg
    21841 acggaccggt cctgcccagc ggcagccgcc tcgacaccgg cacggccttc ctcagcctca
    21901 gcggccccgc cgacgcgctt cacgccgtgt ggaagaccgc ccagaccctg gtggaatacc
    21961 tctcgggcat cgccacctgc gccgccgaac tcctcgccgc tgcacgccgc gcccacccgg
    22021 acgcgggcgt ggtgtgcacc cgcaagaact tccccggcac caagcgcgcc gccctcaagg
    22081 ccatcctgtg cgccggcgcc agcccgcacc gcatcagcct ctccgagacc ctgctgatct
    22141 tccccgagca ccgcgccttt ctgccgccgg acgagtcgcc cgccgccacc ctcgcccacc
    22201 tgcgccgcca gtggcccgag cgcccggtcg tggtcgaggt cgcccaggcc gcggacgccc
    22261 tcgcctgggc cgctgccggc gtcgacatcc tgcagctcga gaaaatgtcc cccgacaccg
    22321 tcgccgccct gcacgcccag atctccgccg gccccggcca cccgcgcctc gccgcggccg
    22381 gcggcatcac gcccgccaac gccgaagcct acgtgcgcgc cggtgcccgc atcctggtca
    22441 ccagcgcccc ctatttcgcc ccgcccaagg acgtggcggt caccctcacc cctgccgacg
    22501 agccccgatc atgaaagtcg ccatcgccac ccagaacttc accattgtca gtggccacgc
    22561 cggccagtcg cgccagtggc tggtgtacga cctgggtgaa caccactcca gccggctcct
    22621 gcccgccccc caacgcatcg agctggccga ggacgaagtg atgcacgtct tcaaggacga
    22681 ccgcccccat cccctggacg gcgtggacgt ggtggtcgcc gccagcgccg gcgacggctt
    22741 cgtccgccac atgcgccacc gccgcgccga cgtcctcctc acccgcgaga gcgaccccgc
    22801 cctcgccatc acccacatcc tcgccggcga agccctgccg gacccgcgct tcgacgtcac
    22861 cacgacgctg tgcaagatcc gggatctgtt ttcgcggtat tgacaacgcc agtactcaaa
    22921 gtccagaaat cgtacctgtt gcccagggaa agccctgaaa actgcaagcg ccctcgtcgt
    22981 ggccgaggct gcaggcaagc gtctcaatca atgggtattg gaggtgttgc agcgcgccgc
    23041 tcatcccgaa gggtgagccg tcttgccccc acggcttcaa tagtgatacc gcaactgcgc
    23101 cagcagcacg gcatcgccct cgatgcggta caccatccga tgttcgtccg tgatcctgcg
    23161 ggaccagaat ccggacaaag cgtgcttcag gggttcgggt tttccccggc cggaaaacgg
    23221 ctcccgtttg acttcttgaa tcagcgtgtt gatccgctcg accatcttgc ggtcctgatg
    23281 ctgccagtac agatagtctt cccacgctgc atccgagaaa atcaacctca cagtgacaca
    23341 tcccgctcaa cgccttgtcc cgcggccaac tgctcgacgg cggagagcag gcgcctggcg
    23401 ttggcggggc tgcgtaggag gtaggccgtc tcttcgaggg cgttgtagtc ttcgagggac
    23461 agcatcacca cggattgctc gccattgcgg gtgatgatca gcggcgcgtg atcctcacac
    23521 acgcgatcca tggtcttggc cagattcgcg cgggccgtgc tgtaggtgat cgcatccatg
    23581 gggggctcct ctgtatatgt acgcgttact gtacaaccta ccccatggtc cggcaagcaa
    23641 tgcctcgccg cccgccctca cacatccagc gcccgccgca gcaccaccgc cacgtgctcc
    23701 gcctcgcgtc cggtgccgtc gaggatctga tgccggcacg agaagccgtc ggcgatgacc
    23761 agggcgtcgg gggcggcctg gcggaccgcg gggagcaggc tcagttcacc catcttcagc
    23821 gaggccgcgt agtgctcggc ctccaggccg aagctgccgg acatgccgca gcagctggcg
    23881 tcgacgatgt cgaactcgaa gccgggaatc cagcccagca ccttgcgggt ggccttcatg
    23941 gcaccgaagg ccttctggtg gcagtggcca tgcaccacgg cgcggccgcc cggaaggggc
    24001 ttgaggggca gcttgaggcc cttgttgcct tcgcgcatga ggaattcctc gaacaggaag
    24061 gcgagcttgc ccagctggcc gacctccggg ccgagggaca gactgtagaa ctcgtcccgc
    24121 agggcaagca ggcaggaggg ctccaggccg accaccggga cgccctcggc gagcaggggc
    24181 accagggcag ccatcgtgcg gcggccctcc tcgcgggcct catccaccag cccctgagac
    24241 aggtagctgc gcccgcagca cagggggcgc tccggttcgt cgtcgccggc ggcggggcgc
    24301 agcacctgga cggcgtagcc gccggcctcc agcacttcgc gggccgcctc ggccacttcg
    24361 ggcgtgtagt agtgggtgaa ggtatcgacg aagagcgcca cgcggccgcg gggggcgtcg
    24421 accggcccgg ggcggtcggc cacaccgtgg ccggtgatgg acgggatgga gcgctgcgcg
    24481 gcgatgccga gccagccttc ggccttcttc gccacccacg gtgaggcgtt gcgcaggcgc
    24541 accagggcgg ccagcacgcc acggtaacgg tgcagccagc ggggcagcgc accgaacagg
    24601 cgggcccgcc gcgacacgcc ctgcacggcg ttgcgctggg ccaggtattc ggtcttgatg
    24661 agggtcatgt ccaccgcgct gg
//
LOCUS       DAOQMM010000047        24607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_638_length_24607_cov_47.727476, whole genome shotgun sequence.
ACCESSION   DAOQMM010000047 DAOQMM010000000
VERSION     DAOQMM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24607
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_24607_cov_47.727476"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2711)
                     /locus_tag="PLH85_09410"
     CDS             complement(<1..2711)
                     /locus_tag="PLH85_09410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNB64821.1"
                     /translation="MPILPPPPYAELCCLTNFTFLTGSSHPEEFVDRARSLGYHALAV
                     TDECSVAGIVRAWDAWRRNPSNTLRLIVGSLIRLEDGPAVVLLATDRAAYGRLAALIP
                     RGRRAAVKGRYRLTRADLEDGLPGCLALLLPPDGPLDPPALANDAHWLATRFPGELWL
                     AAPLLLGADDAARRDTLAAVAAAAGIPVVATATPLMHAPERRRLADVLTALRHRRPLD
                     DAGYRLAANGERHLQPREVLALRHPPAWLAETLAVAARCTFALDSLRYEYPEEIVPPG
                     LTPTSHLRRLVDAGLARRYPPPGTIPAAVRTQVNAELALIAELGYEAFFLTVEDIVRF
                     ARSQGILCQGRGSAANSVVCYALGITEVDPAQATLLFERFISRERNEPPDIDVDFEHD
                     RREEVIQYLYAKYGRDRAALAAAVIRYRPRSALRDVGRALGFDTGQLDQITRRLAWWD
                     GRRVAPERLAEAGLDPASPQVHQLLDLADQLIGFPRHLSQHVGGFVIARGPLAELVPV
                     ENAAMAGRTVIQWDKDDLEALGLLKVDVLALGMLSAIRRSLDLIAAWQGRPFGLPDIP
                     REDPQVYAMLRAADAIGVFQVESRAQLSMLPRLQPRCFYDLVVEVAIVRPGPIQGGMV
                     HPYLQARERLARGESLDYPRPEIRPVLERTHGVPIFQEQVMQLAMVAAGFSAGEADQL
                     RRAMGAWGRKGQLHLYQARLAQGMAERGYPPEFAAALCRQIEGFGSYGFPESHAASFA
                     LLVYVSAWIKRFHPAAFLCALLNSQPMGFYSPSQLIQDARRQGVAVLPADVCTSDGDS
                     TLEAPPGAPQPAVRLGLERIKGIGRDAARRIATARRQAPFASVDDLTRRARLDTADLR
                     ALLAVPASHTILHLQGGAHAQFAAVPLQMLPDARNRTEAVYVITGIPP"
     gene            2823..3941
                     /locus_tag="PLH85_09415"
     CDS             2823..3941
                     /locus_tag="PLH85_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64822.1"
                     /translation="MKRRIGIGLAAALLVTCPTTWATEAQSIEIKVEDIASLKKYPKV
                     ALIGYAIEQQNFLVKMSTSWGSTSSSTSEVTLEGVSTPAMQAATDRLYADLVKRLEAA
                     GLEVVKLDEVRNDPMFADLKGDKPQPSPSETSFVFDKSKGFKNSKALVFSPSGLPWHI
                     PSAHEEAARFGAGDKMSANLSRAFSGKQPVADVENALAKARGITLLKAYYVVGFGRAG
                     GRVSEMTSFNYVSNRSEKTISAAANATAELYLDKTDTRLALRVPGETPMLRMRNNSSP
                     AADGSGFIRLDKKLSAGADFAVGEPKNANSTETQVGNALSTTLAVVGGLAGIRGVGSA
                     STQEFVVTANEQRYVDTVEALIQTVQAEFVDRLAAAAK"
     gene            complement(4061..4843)
                     /locus_tag="PLH85_09420"
     CDS             complement(4061..4843)
                     /locus_tag="PLH85_09420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB64823.1"
                     /translation="MKILPSRLALALLALTATSAVLAADNLATVNGTAIPQARADVII
                     AEQRGQGAPDSDQLRAAVKEDLIRREVLSQEARKKGLEKNASVMAQMDLARQAVLIRA
                     YLQDYVKAHPVSDADVKAEYDRIKAQLGDKEYHARHILVDKEEDAKAIIAKLDKGEKF
                     DELAKQSKDPGSREKGGDLGWANPASFVKPFADAMVKLEKGKYTAAPVKTDFGYHVIR
                     LEDTRALKTPSFEEAKTQLKQRLEQQAVERHITELRGKATVK"
     gene            complement(4877..5140)
                     /locus_tag="PLH85_09425"
     CDS             complement(4877..5140)
                     /locus_tag="PLH85_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNB64824.1"
                     /translation="MSVPDTIRTRLQSLAPLTLDIVDDSALHAGHAGARAGGGHYRLS
                     IVSTAFAGRNTVARHRMIYDALGDMMRHEIHALAIHARTPEEA"
     gene            complement(5137..5436)
                     /locus_tag="PLH85_09430"
     CDS             complement(5137..5436)
                     /locus_tag="PLH85_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNB64825.1"
                     /translation="MLYALIGEDAPGTLDTRMAVRPGHVARLQALQADGRLILAGPCP
                     AVDSPDPGPAGFSGSLIVAEFPTLADAEAWMAEDPYVLEGVFARTVVKPFKKVLP"
     gene            complement(5436..5972)
                     /locus_tag="PLH85_09435"
     CDS             complement(5436..5972)
                     /locus_tag="PLH85_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNB64826.1"
                     /translation="MKLLFDLFPILLFFIAYKFAGIYTATGVAIAATVGQIVWVWFHH
                     RKVDTMLWVSLVLVSVFGGATLIFQNPTFIKWKPTVLYWLFAVVLVGAPLILKRNLIR
                     AMLEAQMHLPDPLWGRLNLAWAGFFAAMGGLNLFVAYSFSEDAWVNFKLFGGMGLMLV
                     FVLAQGMILSRYIEEEPK"
     gene            complement(6027..6437)
                     /locus_tag="PLH85_09440"
     CDS             complement(6027..6437)
                     /locus_tag="PLH85_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNB64827.1"
                     /translation="MPKMPDPALSTPYESFGGEETIRRLVHRFYALMDALPEAWDIRQ
                     MHPADLSGSEQKLFMYLTGWLGGPPLYEEAYGHPRLRARHLPFTIGDTERDQWLLCMR
                     MALDECIPESPLRARILQAIEGLADHMRNRPAGG"
     gene            complement(6421..7131)
                     /locus_tag="PLH85_09445"
     CDS             complement(6421..7131)
                     /locus_tag="PLH85_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB64828.1"
                     /translation="MILFRQFFDTASCTLTYLLADPVTGDAVIIDSVREHVGAYISFL
                     QEQELQLDWILETHVHADHVTGAAALREFTGARSAVGAGGLVGCADRALEDGDSIVFG
                     NQVIRAIATPGHTPGCMSYHWRDRLFTGDALLIGGCGRTDFQGGDAGQLYDSITERLF
                     TLPDETLVYPGHDYRHARVSCIGQEKLANPRFAGKSRADFIELMGHLNLPKPTLMDQA
                     VPANLHCGRLAPLDAQDA"
     gene            complement(7170..8534)
                     /locus_tag="PLH85_09450"
     CDS             complement(7170..8534)
                     /locus_tag="PLH85_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64829.1"
                     /translation="MAIRPSGYRRFFLHAPRTLLWRIFLLVAALILVSVAMWSAIFNH
                     FDEAGRARQIAQTVSSIVNLTRSALVNADPALRRELLMDLATLEGIRIYPAEKQDRME
                     PLADTSLFHEITAEVRRQLGPDTRFASAWEGLPGFWVSFRLEGETPEDSTDFWVLLPP
                     ERVIRPHTREWLGWGAGALCLAMLGAYVIVSRLNRPLRQMAAAAAMVGRGETPPRLDE
                     DGPLELSDLARTFNQMSRDLQELDADRALILAGVSHDLRTPLARLRLGIEMSGASSAD
                     VDAMVSDIEDMDRIIGQFLEFSRSDGGEPLDTVALHELARELSTHYARRGTQLSIAAE
                     QPAFARARPQALRRAVINLVENALRYAGDSPALEVGIEQGQPCIAVLDRGPGIPPADV
                     ERVKRPFTRLDSARGNTKGSGLGLAIVDRIARGQQGELLLLPRDGGGLRAVIRLPAAP
                     KTAG"
     gene            complement(8542..9267)
                     /gene="ompR"
                     /locus_tag="PLH85_09455"
     CDS             complement(8542..9267)
                     /gene="ompR"
                     /locus_tag="PLH85_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HNB64830.1"
                     /translation="MEKESYRILVVDDDVRLRDLLKRYLGEQGFNVKTVSDAAQMDKA
                     LLREHFDLMVLDLMLPGEDGLAICRRLRGTENTIPIVMLTAKGDDVDRIVGLEMGADD
                     YLPKPFNPRELVARIHAVLRRQPQTPPGAPATEDEDVSFGSVVVQLGTRILVRNGEET
                     LLTTGEFALLKVLLQHPRQPLSRDRLMELARGREYDVFDRSIDVQVSRLRKLIEDDPG
                     KPRYIQTVWGFGYVFVPDGNKHG"
     gene            9508..9828
                     /locus_tag="PLH85_09460"
     CDS             9508..9828
                     /locus_tag="PLH85_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64831.1"
                     /translation="MNRFSPTRFAHLRLGRRLLAGGTLVLILVSAAHADERRREAFIR
                     PPGPGQERPTRQIPAFEMDTLRRQEFPEGREPSRRWTSEERRQLRRDVHEAGRDLYVD
                     APRP"
     gene            9907..10596
                     /gene="tsaB"
                     /locus_tag="PLH85_09465"
     CDS             9907..10596
                     /gene="tsaB"
                     /locus_tag="PLH85_09465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNB64832.1"
                     /translation="MKILAIESSTEAASVALLCGDVLLERDVPGRTHSETLLPAIRAL
                     LGEAGCPLNALDGVAFGAGPGAFTGLRLACGAAQGLAMGIDRPVVPVGTLEALASLYE
                     GDRIFVAMDARMAEVYFAAYRRVGTRLDEVLAPACARPEDVRLPASGSWAGVGSAFAA
                     YPDRLLPGLGDRLLAVDAAVQPRAGAVARLAAVRLARGEGLDATLAAPLYVRDKVALT
                     TAERLAQGGRA"
     gene            10623..11045
                     /gene="rimI"
                     /locus_tag="PLH85_09470"
     CDS             10623..11045
                     /gene="rimI"
                     /locus_tag="PLH85_09470"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNB64833.1"
                     /translation="MAETDLSWVAEQDRVLYPFPWVEQNFSDSMAAGYSCWLMRAGDA
                     PVGYAVLMRVLDEAHILNISVAADCQGRGFGRRLLGHLCAVALAAGGRQMFLEVRPSN
                     ASALALYARAGFETIGRRKGYYPAPEGREDALVMRLPL"
     gene            11042..11941
                     /locus_tag="PLH85_09475"
     CDS             11042..11941
                     /locus_tag="PLH85_09475"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNB64834.1"
                     /translation="MSGRRDSVLREMGLAPLWRLRDRPAVDDACGQLADAVVDRAPLQ
                     AERSADTVPAAAGAVDLRIVPPLHSDARRGHAGLPPLAAGVAPAVTPEHVADGRIEGL
                     DWDALEATIRTCEACVLCQKRKQAVPGVGDRQADWLFVGEGPGAEEDERGEPFVGQAG
                     RLLDAMLASIDLRRGHDVYIANAVKCRPPHNRTPETAEIAACFPYLERQIALLQPRLI
                     VALGRPAAQALLNQEIKIGASRGRLFRYGGIPVIVTYHPAYLLRNLHDKGKVWEDLCF
                     ARRTMAELHARADAGAPENLPTI"
     gene            11969..12580
                     /locus_tag="PLH85_09480"
     CDS             11969..12580
                     /locus_tag="PLH85_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNB64835.1"
                     /translation="MRFKLLLSMLLSVLLLSGCGYNQIQVNDEQVNAAWSEVLNQYKR
                     RADLIPNLVQVVQGYAEHEKDVLIKVTEARASVAGVKATPELVNDPEAMARFQKAQGD
                     LSGALSRLLVVSENYPNLKADANFRDLQAQLEGTENRITVARNRYIKAVQDYNVSVRT
                     FPNNLTAMVVGAKPKANFAVDNEQAISEAPKVNFGAPAKPAAQ"
     gene            12618..13523
                     /locus_tag="PLH85_09485"
     CDS             12618..13523
                     /locus_tag="PLH85_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNB64836.1"
                     /translation="MNLRARLLAVCVFICAFVAGAWAEDLKPIPPLAARITDTVGLLP
                     GANRAALEARLADLEARKGAQVAVLIVASTQPEDIAAYSIRVVEAWKLGRTGVDDGVL
                     FLIARDDRRMRIEVGRGLEGAIPDAVAKRIVAERVAPRFKAGDFPGGIEAGLEALVTQ
                     IEGEEALPPPAPAATQGNDLEDFQGPAFIGCVVFAFIGGAILKAIFGRLIGATLTGGV
                     IAAVIWAVTAALVFGLIAGAIGFIFTLGAGGARGRHVGGSSWSGGGFGGAGGFGGGSS
                     DSSDSGGGWSGGGGDFGGGGASGDW"
     gene            13571..14020
                     /locus_tag="PLH85_09490"
     CDS             13571..14020
                     /locus_tag="PLH85_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNB64837.1"
                     /translation="MRRAFTSTVLSAIETAIRRSEATHRGEIRFAVEGGLAWSDLRRG
                     LTARQRAAKVFSDLGVWDTAGNTGVLIYVQWVDRRVEILADRGIAACVPQAEWNAVCH
                     GLEQAFKAGRYAEGCTAAVDAVGQLLATHFPAGADNPDELPDRPVLL"
     gene            complement(14030..17230)
                     /locus_tag="PLH85_09495"
     CDS             complement(14030..17230)
                     /locus_tag="PLH85_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB64838.1"
                     /translation="MTLPPVPASSGTMTGAGVRPAQPERCALVAAGLYAFTATAWLLL
                     TEHTAEGAAGVLIQDGLLLVGVTALGLYLGIRHFLAQVTERAAACEATAQAYRAFFEF
                     VPDAVWFFDTRSRRIVAANPAAGRLYGRLASQMEGADVATVLADSTSAEWNPGPCLLG
                     HRLADGTIRALDTTLYDQVFAGHPCRVVYVQEAAISRALPNRLEPSIADILDKVPLAL
                     YRYGAAHEHYDYVSARMTEWLGVSADALGQPGGWKHFAERVPDDDLGALWARIDAALA
                     QPGTGPVDLTVTYRLHTAGGALLHIQDAMTLLRNPDGSLRAIAGAASDHTAAQQAGEY
                     LGVTLRSIGDGVIATNAAGNITLMNTAACVLTGWTEQDAVGKPLRVVLHLEPPTDGPA
                     SDRGLPRQIRLVDRVGEVRTVACCRTPIRGSASDLPLGEVIVLHDLTDERTVRDSAAG
                     DRARYLALVENSPVGILRFSRDLRIVYCNTRLATLFRSTPDQLVGLDLHGLEDDTVLA
                     TLRDVLSGQPGAYDGPFRSTMLPAPLNLSIRVTPELDADGEVIGGIGTVEDRTAFLET
                     EAQLRDTRERYVLAQRGTNEGLWDWDPRTRHLYLSARLLSLLGLTSDTLRTSGQEWQK
                     LIHPDDRPHVRADFIAHLRGRTEHFESEYRIADKDGRYHWVHARGLAHRNRRGRPVRV
                     VGSIGDITARKQAEEQLKAERDFSRSLIDSLPAPFFLFDQAGRLVLWNSFVKDLTGLS
                     DDELHHAEVEKLVVPEQEPILEHRLQVALTSGQASAEVLLRRRDKDPVPFLVVGRRVI
                     LDGVPHIIGVATDLTERKFAERAIKRLNTELERRVNERTSQLSAALSELESFSYSVSH
                     DLRAPLRAIEGYSTILGSDYADRLDDEAKELLRRVRAAVHRMGQLIDDLLTLSRVSRK
                     ELERRPVDLSLLAQAICDELRQQEPGRHVIVEIAPDLKVDADPSLMRTVLENLLGNAW
                     KFTGRVEVPEIRFSAVRHEGMDAFVVQDNGAGFDMRYRENLFRPFQRLHSDREFPGTG
                     IGLATVARIVRRHGGQVWAEGEVGKGAALYFSIGQS"
     gene            17330..17815
                     /gene="tadA"
                     /locus_tag="PLH85_09500"
     CDS             17330..17815
                     /gene="tadA"
                     /locus_tag="PLH85_09500"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNB64839.1"
                     /translation="MTESDLDRDTAYMRQAIELAREAGRHGEVPVGALVVGDGGIVGR
                     GFNRPIGTHDPTAHAEIMALRDASARLANYRIPGHTLYVTLEPCAMCAGAILHARIAR
                     VVFGATDPKTGVSGSVLDLFSEKRLNHHTTVVGGILAEECGGLLRDFFAMRRNKSVSG
                     A"
     gene            17819..18298
                     /locus_tag="PLH85_09505"
     CDS             17819..18298
                     /locus_tag="PLH85_09505"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNB64840.1"
                     /translation="MQILIDIPSQWLSLLADDGTCLRRYRVSTARNGPGERSGSHCTP
                     RGRHVIRARIGAGQPLGAAFRGRRPTGEVWSPEMAAAQPERDWILTRILWLSGREPGR
                     NRWGDVDSMRRYIYIHGTPDTEPMGVPLSIGCVRMRNRDVVELFDLVPAGTPVEIRS"
     gene            18295..18738
                     /locus_tag="PLH85_09510"
     CDS             18295..18738
                     /locus_tag="PLH85_09510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB64841.1"
                     /translation="MSPAQDAVPEVILGAWDVLRDAACHIRFAVFVDEQGVPAEMEVD
                     EADPSSCHALAWIGGAAVGTGRLLPDGHVGRMAVLPAWRGRGVGRAVLTALMAEARRR
                     GLSRLVLHAQTHAEGFYAGFGFVAEGEVFDDAGLPHRTMVWQVQG"
     gene            complement(18822..19496)
                     /locus_tag="PLH85_09515"
     CDS             complement(18822..19496)
                     /locus_tag="PLH85_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB64842.1"
                     /translation="MKLRHTPLSIAAGPVWLDGLLAHAPDARGLVVIAQNAAGHHQDS
                     REAHFAARIEAAGFATLIFDLLTRYEETRDPDTRYNAPLLGQRLNALFEWIRHQPPLE
                     GLAVGLVTSGNGCAAAIRAMSREPEFIAALVCRGGRPGLAGADPLRLVQSPACMIVGE
                     RDEGIAGVRQAYELLGGPKAWREIPGTDGLFREPGTLDEAASHAADWFLRHLPPPAGQ
                     APAMTA"
     gene            complement(19561..20670)
                     /gene="amrS"
                     /locus_tag="PLH85_09520"
     CDS             complement(19561..20670)
                     /gene="amrS"
                     /locus_tag="PLH85_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNB64843.1"
                     /translation="MEGIGGPDALNGVPARYWHRLDDGRIQCDLCPRDCRLHAGQRGA
                     CFVRQRVGDGLVLTTYGRSSGFCIDPIEKKPLNHFHPGSSVFSFGTAGCNLACKFCQN
                     WDISKSRDMDRLMDAAAPDEIAATAAAHRCRSVAFTYNDPVIFAEYAIDTAVACHARG
                     LATVAVTAGYISPAPRRDFFAVMDAANVDLKAFTDDFYVHQTGARLAPVLDTLLYLRH
                     ETDVWVEITTLLIPGLNDSDAELSALSRWVADELGTDVPLHFTAFHPDYKLDTLPPTP
                     PATLRRALRIALDAGLAYVYTGNVRDIEGASTRCPACGQTLIERDGYRILAYRLDAHG
                     ACTACGHVIAGHFGDFTGAFGPRRVPVHIHRHAGP"
     gene            complement(20640..21206)
                     /gene="amrA"
                     /locus_tag="PLH85_09525"
     CDS             complement(20640..21206)
                     /gene="amrA"
                     /locus_tag="PLH85_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNB64844.1"
                     /translation="MPATDALGPVLLAHARAAIADALGLPHAPAPEDPRLSERRASFV
                     TLTRNGELRGCIGSLSAHRPLGEDVRANAAAAALKDSRFPPLDAREFDAVRVEVSVLT
                     APEFLEFRDEADALSQLVPGRDGVIFFNGCQKATFLPQVWAQLPEPRAFLAALKQKAR
                     LPVDFWGPNVMIARYEVEKWKESEVPTP"
     gene            complement(21193..22011)
                     /gene="amrB"
                     /locus_tag="PLH85_09530"
     CDS             complement(21193..22011)
                     /gene="amrB"
                     /locus_tag="PLH85_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNB64845.1"
                     /translation="MAHASQRPPAVAGLFYPADPHSLHAQISGLLAGAVPAERIDCPK
                     ALVVPHAGYIYSGAVAATAYEALARRRGVLRRVVILCPTHRVAIRGMALPASLSFITP
                     LGAVPVDQDAWRLAQTLPDVVVDDRPHAQEHAIEVQLPFLQCVLDHFAIVPIAVGQAD
                     AASVAAAIEALWGGPETLILVSSDLSHYHPYRQAQLRDKATLAQIQQLDPRIEPEQAC
                     GAGPINGLLLAALRHHLRPQVLDQRNSGDTAGDRNRVVGYAAIAFSEDLDHACH"
     gene            complement(22053..23330)
                     /locus_tag="PLH85_09535"
     CDS             complement(22053..23330)
                     /locus_tag="PLH85_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNB64846.1"
                     /translation="MNTLLFHQAFDPAQLEALAVATAIGLLMGLERERHPASRAGVRT
                     FGLTGLLGALCALLAERLGTPGVLVAGLVLVAVMMIAAYLRRPDPHDPGTTSVIAILV
                     CFALGAMVWLGFGRIAVMAAVGATMLLYFKSELSGVAARLTAQDWRSILQFAVLSLIV
                     LPILPDRGFGPYEALNPHQIWLMVVLIAGVSLAGYAALRLAGARLGAPLLGLLGGLVS
                     STATTMVFSRHARENPAMVSTAKLVILLANLVMVVRVGGIALAVAPSVAPRLWPGAGL
                     ALLAGAAVLAWNWRQLVHQGELPIPDTRNPTELRAAMGFGLLYALVLLCAAWLSDIAG
                     QGGLYMLALASGLTDVDAITLSMLRLLNTGKLGADPAATGILLAMLANLSFKSGLALS
                     LGGRHLGSGVAAGMAAVGAGLGGGIAWLHLASL"
     gene            23402..24109
                     /gene="ispD"
                     /locus_tag="PLH85_09540"
     CDS             23402..24109
                     /gene="ispD"
                     /locus_tag="PLH85_09540"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNB64847.1"
                     /translation="MHTHHSRCFALVPAAGSGSRMKSEQPKQYLSLLGEPLIRHTLAV
                     LCASPLLERVYVVLSVGDQEWDRHPWHSFGDKLVPLFCGGASRADSVLNGLRAIADDT
                     ASSDWILVHDAARPCLAPWHIESLVRELIHDEVGGILAVPVADTLKRADSQQRIAATV
                     PRDGLWQAQTPQMFRYVMLKRALEAARDVTDEASAIEAMGLRPRLVRGDPTNLKVTYP
                     LDLHLAEWILQHREGLA"
     gene            24106..24597
                     /gene="ispF"
                     /locus_tag="PLH85_09545"
     CDS             24106..24597
                     /gene="ispF"
                     /locus_tag="PLH85_09545"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNB64848.1"
                     /translation="MIVPFRIGQGFDVHALVPGRALILGGVTIPHTHGLLGHSDADVL
                     LHALTDAILGAAGLGDIGRHFPDTDPRFEGADSRVLLREAAAAVRRAGWQTVNVDATV
                     IARAPKILPHVPAMVACIAADLEVAPEAVNIKGKTTEKLGFTGRGEGIAAQAVALLAR
                     VET"
BASE COUNT         3849 a         7680 c         9208 g         3870 t
ORIGIN      
        1 ggggggatgc cggtgatgac gtacaccgcc tcggtgcggt tgcgcgcgtc gggcagcatc
       61 tggagcggca cggctgcgaa ttgcgcgtgg gcccccccct gcaggtggag gatggtgtgg
      121 gaggctggga cggcgaggag ggcgcgcagg tcggcggtgt ccaggcgggc acgacgggtg
      181 agatcgtcca ccgatgcaaa cggggcctgg cggcgggccg tggcgatgcg tcgggcggca
      241 tcccggccga tgcccttgat gcgctccagg ccgaggcgca cggcgggctg cggggcgccg
      301 ggcggggctt cgagggtgct gtcgccgtcg ctggtgcaga catcggcggg cagcaccgcc
      361 acgccttggc ggcgggcgtc ctggatgagc tgggaggggc tgtagaaacc catgggctgg
      421 ctgttgagca gcgcgcacag gaaggctgcg gggtggaagc gtttgatcca cgcggacacg
      481 tacaccagca gcgcgaagct ggcagcgtgg gattcgggga agccgtagct gccgaagcct
      541 tcgatctggc ggcacagggc ggcggcgaac tcgggggggt agccccgctc ggccatgccc
      601 tgggccagac gggcctggta taggtggagc tgacccttgc ggccccaggc gcccatggcg
      661 cgccgcagct ggtcggcttc gccggcgctg aagccggcgg cgaccatggc gagctgcatg
      721 acctgttcct ggaagatggg cacgccgtgg gtgcgttcga gtacggggcg gatctcgggg
      781 cgggggtagt caagggattc gccgcgggcg aggcgctccc gcgcctgcag gtagggatga
      841 accatgccgc cctggatcgg accgggtcgc acgatggcca cttccaccac cagatcgtag
      901 aagcagcgtg gctgcaggcg gggcagcatg ctcagctggg cgcgggattc cacctggaac
      961 acgccgatgg cgtcggcggc gcggagcatg gcgtagacct gcgggtcctc gcgggggatg
     1021 tcgggcaggc cgaaggggcg gccctgccag gcagcgatga ggtccaggct gcgccggatg
     1081 gcggacagca tgcccagggc gaggacgtcc accttgagca ggccgagggc ttccaggtcg
     1141 tccttgtccc actggatgac ggtgcgcccg gccatggcgg cgttttcgac cggcaccagc
     1201 tcggccaggg ggccgcgggc gatgacgaag ccgcccacgt gttgggacag gtgacgggga
     1261 aagccgatga gctgatcggc cagatcgagg agttggtgca cctgcgggct ggccggatcg
     1321 agaccggctt cggcgaggcg ttcgggggcg acgcggcggc cgtcccacca ggcgaggcgg
     1381 cgggtgatct ggtcgagctg accagtgtcg aagccgaggg cgcggcccac gtcccgcagg
     1441 gcgctgcggg ggcgatagcg gatcaccgcg gcggccaggg cggcgcggtc gcgaccgtac
     1501 ttggcgtaca ggtactggat gacctcctcg cgccggtcgt gctcgaaatc cacatcgatg
     1561 tccggcggct cgttgcgttc ccgggagatg aagcgttcga agagcagggt ggcctgggcc
     1621 gggtcgacct cggtgatgcc aagggcgtag cacaccacgg agttggccgc cgagccgcgc
     1681 ccctggcaca ggatgccctg gctgcgcgcg aagcggacga tgtcctcgac ggtgaggaag
     1741 aaggcttcat agccgagttc ggcgatcagg gcgagttcgg cgttgacctg ggtgcggacg
     1801 gcggcgggga tggtgccggg tggcgggtag cggcgcgcca ggccggcatc caccaggcgg
     1861 cgcaggtggc tcgtgggggt gaggccgggc gggacgattt cctcgggata ttcgtagcgc
     1921 aggctatcga gggcgaaggt gcagcgggca gccacggcga gggtctccgc cagccaggcg
     1981 ggtggatgac gcagggcgag gacctcacgg ggctggaggt ggcgttcgcc gttggcggcc
     2041 aggcggtagc ccgcgtcgtc gaggggcctg cggtggcgca gggcggtaag gacgtcggcc
     2101 aggcggcggc gctcgggggc atgcatcagc ggggtggcgg tggcgaccac gggaatcccg
     2161 gcggcggcgg cgaccgcggc gagggtgtcg cgtcgggcgg cgtcatcggc gccgagcagg
     2221 agcggggcgg cgagccagag ctcaccggga aaacgcgtgg cgagccagtg ggcgtcgttg
     2281 gcgagcgccg ggggatccag cgggccatcc ggcggcagga gcagggcgag acagccgggc
     2341 agaccgtcct ccagatcggc acgggtgagg cggtagcgtc ccttgacggc ggcccggcgg
     2401 ccacggggga tgagggcggc cagacgcccg taggcggccc ggtcggtggc gagcaggacg
     2461 acggcaggcc cgtcttccag gcggatgagg ctcccgacga tgagtcgaag ggtgttcgac
     2521 gggttccgtc gccaggcgtc ccaggcgcgc acgatgccgg cgacggaaca ttcgtctgtc
     2581 actgccagtg cgtggtaacc cagtgatcgt gcacggtcga cgaactcttc cggatgggaa
     2641 gatcctgtca ggaatgtaaa attcgtgagg cagcacaact cggcataggg aggcggaggg
     2701 aggattggca taggaaaact gtacaaaaga acagtttttc ggtcaatctc acatgccccg
     2761 catcgacctg tcgcgccggg ttgggcttgt tcgacaacac atcatcccgg agcacgacat
     2821 ccatgaagcg tcgaatcggt atcgggctgg ccgcagcctt gctggtcacc tgtcccacga
     2881 cctgggcgac ggaagcccag tccatcgaga tcaaggttga agacatcgcc tccctcaaga
     2941 agtacccgaa ggtggccttg attggctatg ccatcgagca acagaacttt ctggtcaaga
     3001 tgtccacgag ttggggcagc acgtcatcca gcacctcgga agtgaccctc gaaggggttt
     3061 cgacacccgc catgcaggcg gccaccgacc ggttgtatgc cgatctggtc aagcgcctcg
     3121 aggcggcggg gctggaagtg gtgaaactgg acgaggtgcg caacgacccg atgtttgcgg
     3181 acctcaaggg cgacaagccg cagccgtccc cctccgagac gagcttcgtg ttcgacaaat
     3241 ccaagggatt caagaacagt aaggcgctgg tgttctcgcc cagcggtctg ccctggcaca
     3301 ttccttccgc ccacgaagaa gcggcccgct tcggcgcggg cgacaagatg tcggccaacc
     3361 tgtcccgtgc cttctccggc aagcagcccg tggccgacgt ggagaatgcc ctggccaagg
     3421 cccgtggcat caccctgctg aaggcctatt acgtcgtggg cttcggccgc gccggcggac
     3481 gggtgtcgga aatgacgtcc ttcaactacg tgagcaatcg cagcgaaaag acgatttcgg
     3541 cagcagccaa cgcgacggcg gagctctatc tggacaagac ggatacccgt ctggccctgc
     3601 gggtgccggg cgagacaccg atgctgcgca tgcgtaacaa cagctcgccg gctgccgatg
     3661 gcagcggttt catccgcctg gacaagaagc tgagcgccgg ggcggacttc gcggtgggtg
     3721 aaccgaagaa cgcgaattcg acggaaaccc aggtgggcaa cgccctctcg accaccctgg
     3781 cggtggtggg cgggctcgcg ggcatccgcg gcgtgggttc ggccagcacg caggaatttg
     3841 tggtgacggc caacgaacag cgctacgtgg atacggttga ggccttgatc cagacagtgc
     3901 aggccgagtt tgtggaccgc ctggccgccg cggccaagta aatctgcccg ggcgggcgtc
     3961 gattgccacg acgccgcccg ctgcgggcgc catccgtaaa aagccgaccg gaatgacccg
     4021 gtcggctttt ctttgagggc cgcctgctgc agggcgatcc ttacttgaca gtggccttgc
     4081 cgcgcaattc cgtgatgtgg cgttcaacgg cctgctgctc gagacgctgc ttgagctgcg
     4141 tcttggcttc ctcgaaggac ggcgtcttga gcgcgcgggt gtcctcgagc ctgatgacgt
     4201 ggtagccgaa gtccgtcttc acgggggcgg ccgtgtattt gcccttttcg agcttgacca
     4261 tggcatcggc gaacggcttc acgaagctcg cggggttggc ccagcccaga tcgccgccct
     4321 tttcgcggga gcccggatcc ttggactgct tggccagttc gtcgaacttc tcgcccttgt
     4381 cgagcttggc gatgatggcc ttggcgtcct cttccttgtc caccaggatg tggcgggcgt
     4441 gatattcctt gtcgcccagc tgggccttga tgcggtcgta ttcggccttc acgtcggcgt
     4501 cgctgacggg gtgcgccttg acgtagtcct gcaggtaggc gcggatcagc acggcctggc
     4561 gggcaagatc catctgggcc atgacgcttg cgttcttttc gagacctttc ttgcgggctt
     4621 cctgggacag cacctcgcgc cggatcaggt cttccttgac cgccgcacgc aactgatcgg
     4681 agtcgggcgc gccctggccg cgctgctcgg caatgatgac gtccgcacgg gcctggggaa
     4741 tggctgttcc attgacggtg gccagattgt cggcagcgag gacggcggag gtggccgtca
     4801 gggccagcag ggcgagcgcc agacggctcg ggagaatctt catcggatgt tttccttggg
     4861 tgtgggacag ttgaaatcag gcttcttcgg gcgtcctggc gtggatggcc agggcgtgga
     4921 tctcgtggcg catcatgtcg ccgagggcgt cgtagatcat gcggtgccgc gccaccgtgt
     4981 tgcggccagc aaaggcggtg gagacgatgc tcaggcggta atggccgcct cctgcacggg
     5041 cgccggcatg gccggcatgc agggcgctgt cgtcgacgat gtcgagggtc agcggggcaa
     5101 ggctctgcag gcgggtgcgg atggtgtccg gcacgctcac ggcaggacct ttttgaaggg
     5161 cttgacgacg gtacgggcga acacgccctc cagcacgtag gggtcttcgg ccatccacgc
     5221 ctcggcgtcg gccagggtcg ggaattcggc gacaatcagg ctgccgctga agccggcggg
     5281 gcccggatcg ggcgaatcca cggccgggca ggggccggcc aggatcaggc ggccatcggc
     5341 ctgcagggcc tgcaggcggg ccacatggcc cggccgtacg gccatgcggg tgtccagggt
     5401 gccgggggcg tcttccccga tgagggcgta aagcattact tgggttcctc ttcgatgtaa
     5461 cgggacagga tcatgccttg ggcgaggacg aacacgagca tcagccccat gccgccgaac
     5521 agcttgaagt tgacccaggc gtcctcggag aatgaatagg cgacaaaaag gttgagaccg
     5581 cccatcgcag cgaaaaatcc ggcccaggcc aggttgagcc ggccccacag ggggtcgggc
     5641 aggtgcatct gggcttcgag catggcccgg atcaggttgc gcttgagtat cagcggcgcg
     5701 ccgacgagga cgaccgcgaa gagccagtac aggaccgtgg gcttccactt gatgaaggtg
     5761 ggattctgga agatcagggt cgcaccgccg aagaccgaga cgagcaccag gctcacccac
     5821 agcatggtgt caaccttgcg gtggtgaaac cagacccaga cgatctggcc cacggttgcg
     5881 gcgatggcga ccccggtggc cgtgtagatg ccggcaaact tgtaggcaat gaagaacagc
     5941 aggatgggaa acaggtcgaa aagcagtttc atcggcaggc agtgggattg aggcggggcg
     6001 ggcagggccc ggtgagtcgg aggaggtcag ccgcccgcgg ggcggttacg catgtggtcg
     6061 gcgaggcctt cgatggcctg caggatgcgt gcccgcaatg gggattcggg aatgcactcg
     6121 tccagggcca tgcgcatgca caacagccat tggtcgcgtt cggtatcgcc gatggtgaag
     6181 ggcagatggc gggcacgcag acgcggatgg ccataggctt cctcgtagag cggagggccg
     6241 ccaagccagc cggtgagata catgaaaagc ttctgctcgg agcccgagag atccgccggg
     6301 tgcatctgac ggatgtccca ggcctccggc agcgcgtcca tcagtgcgta gaagcgatgc
     6361 accagacgac ggatcgtctc ttccccgcca aaggactcgt agggcgtgga cagggccggg
     6421 tcaggcatct tgggcatcca gcggcgccag gcggccacag tgcaggttgg ccggcacggc
     6481 ctggtccatc agggtgggct tgggcagatt gaggtggccc atcagttcga tgaagtcggc
     6541 gcgggacttg ccggcaaagc gggggttggc gagcttttcc tggccgatgc aggagacacg
     6601 ggcatggcgg tagtcgtggc cgggatagac gagggtctca tcgggcagcg tgaacaggcg
     6661 ttcggtgatg ctgtcgtaga gctgtcccgc gtcaccaccc tgaaagtcgg tgcggccgca
     6721 gccgccgatg agcagggcgt cgccggtgaa caggcggtcg cgccagtggt agctcatgca
     6781 gccgggcgtg tggccggggg tggcgatggc gcggatcacc tggttgccga agacgatgga
     6841 gtcaccgtct tccagggcac ggtcggcaca gcccacgagg ccacccgcgc ccacggcgct
     6901 gcgggcaccg gtgaactcgc gcagggctgc cgcgccggtg acgtgatcgg cgtggacatg
     6961 ggtttccagg atccagtcga gctgtagctc ctgctcctgc aggaaggaaa tgtaggcgcc
     7021 cacgtgttcg cgtaccgagt cgatgatcac ggcatcgccc gtgacggggt cggcgagaag
     7081 ataggtcagc gtgcagctgg cggtatcgaa aaactgtcgg aacaggatca tggtggcgag
     7141 cgatggacga tgaatgcctg attctagctt caaccggcgg ttttgggcgc agcaggcagg
     7201 cggatgacgg cccgcaggcc accgccgtcc cgtggcagca ggagcaattc tccttgctgg
     7261 ccgcgcgcga tgcggtcgac gatggccagt cccaggcccg agcccttggt gttgccgcgg
     7321 gcggagtcga ggcgggtgaa gggacgtttc acccgctcca cgtcggctgg gggaatgccg
     7381 ggaccccggt cgaggaccgc gatgcacggc tgtccctgct cgatgcccac ctccagcgcg
     7441 gggctgtccc ctgcgtagcg cagggcattc tccaccagat tgatgacagc gcggcgaagg
     7501 gcctgggggc gggcgcgggc gaaggccggc tgttcggcgg cgatgctcag ctgagtgccg
     7561 cggcgggcgt agtgggtgga gagctcccgc gcaagctcgt gcagggcgac ggtgtcgagg
     7621 ggttcgccgc cgtcgctgcg ggaaaattcc aggaactggc cgatgatgcg gtccatgtcc
     7681 tcgatgtcgg agaccatcgc gtccacgtcc gcgctgctgg cgccgctcat ctcgatgccc
     7741 aggcgcaggc gggccagggg tgtccgcaga tcgtgggaca cgccggcgag gatcagcgcc
     7801 cggtcggcgt ccagctcctg caggtcgcgg gacatctggt tgaacgtgcg ggccaggtcg
     7861 gacagttcca gcgggccgtc ctcgtccagg cgcggcggtg tctcgccgcg tccgaccatg
     7921 gcggcggcgg cggccatctg gcgcaggggg cggttgaggc gcgacacgat gacgtaggcg
     7981 cccagcatcg ccagacacag ggccccggcg ccccagccca gccattcccg cgtatgcggg
     8041 cggatcaccc gctcgggcgg cagcaggacc cagaaatcgg tgctgtcctc gggcgtctcg
     8101 ccttccaggc ggaagctcac ccagaagccg ggcaggccct cccaggcgga cgcgaagcgc
     8161 gtgtccggtc cgagctgacg gcgtacttcg gcggtgatct cgtggaacag ggaggtgtcc
     8221 gccaggggtt ccatgcggtc ctgcttctcg gccggataga tgcgaatgcc ttccagggtg
     8281 gccagatcca tcagcagttc gcggcgcagg gccgggtcgg cattgaccag ggcgctgcgg
     8341 gtgagattga cgatgctcga caccgtctgg gcgatctggc gggcccggcc ggcttcgtcg
     8401 aaatgattga agatggccga ccacatggcc accgagacga ggatcagggc cgcgacgagc
     8461 aggaagatgc gccacagcag ggtgcggggc gcatgcagga agaaccgccg gtaaccgctc
     8521 gggcggattg ccacgggccg cttatccgtg cttgttgccg tcggggacga agacatagcc
     8581 gaaaccccac acggtctgaa tgtagcgggg cttgccgggg tcgtcctcga tgagcttgcg
     8641 caggcgcgac acctgcacgt cgatggagcg gtcgaagacg tcgtactcgc ggccgcgggc
     8701 gagctccatc aggcggtcac gggacagggg ctggcgcgga tgctggagca gcaccttgag
     8761 cagggcgaat tctccggtgg tgagcagggt ctcctcgccg ttgcgcacca ggatgcgggt
     8821 gccgagctgc accaccacgc tgccgaaact gacgtcctcg tcttcggtgg ccggtgcgcc
     8881 ggggggcgtc tggggctggc ggcgcagcac ggcgtggatg cgggcgacca gctcgcgcgg
     8941 gttgaagggc ttgggcaggt aatcgtcggc gcccatctcg aggccgacga tgcggtccac
     9001 gtcgtcgccc ttggcggtga gcatcacgat ggggatcgtg ttctcggtgc cgcgcaggcg
     9061 tcggcagatg gccaggccat cctcgccggg cagcatcagg tcgaggacca tcaggtcaaa
     9121 gtgttcgcgc agcagcgcct tgtccatctg ggcggcgtcg ctcaccgtct tgacgttgaa
     9181 accctgctct ccgaggtagc gtttgagcag gtcgcgcagg cgaacgtcgt cgtcgacgac
     9241 cagaatccgg tagctttctt tttccatgga cgcatcctag cggctcggcg gatgggcaac
     9301 cagtgtccca ccgcgcaacg ggtaagcaaa cgtaagggtc ggcgcgggcc taacaatccc
     9361 ttacaaaggg tgtggggcgg cccccggcag gctgccagaa tggacgtacg tgtcaatctc
     9421 ccacacgttc cgtccgtaat gcggggctgc gagtgttttt gcggtgccgc gcgcggcaac
     9481 tcgggttagc attgggaccc catgaaaatg aacaggtttt cccccacgcg attcgcgcat
     9541 ctgcgccttg gtcggcgtct gctggccggc gggacgctgg tgctgatcct cgtttcggcc
     9601 gcccatgcgg acgagcgacg gcgggaagcc ttcattcgac cgccggggcc gggccaggag
     9661 cggccgacac gccagatccc cgccttcgag atggacaccc tgaggcgcca ggaattcccg
     9721 gaaggccgcg agccgtcccg gcgctggact tcggaggagc ggcgccagct gcgtcgtgac
     9781 gtgcacgagg ccggccgcga cctttacgtg gatgcacccc ggccctagct gcgtgaggcc
     9841 ggcgggcccg gttcgccgct gtgcccgttg gtggcgcccg ccgcgggcga tagaatgccg
     9901 cccgacatga agatacttgc catcgaatcg tccaccgaag ccgcgtcggt ggcgctcctc
     9961 tgcggggatg tcctgctcga acgggatgtg cccggccgga cccactccga aaccctgctg
    10021 cccgcgatcc gggcgctgtt aggcgaggcc ggatgcccct tgaatgcgct ggacggcgtg
    10081 gccttcggcg ccgggcccgg cgcattcacc gggctgcgtc tcgcttgcgg ggcggcccag
    10141 ggactggcga tggggattga ccggccggtg gtgccggtcg gcacgctgga ggcgctggcc
    10201 agcctgtatg aaggcgaccg gatctttgta gcgatggacg cgcgcatggc cgaagtctat
    10261 ttcgctgcct accggagagt cgggacacgc ctcgacgagg ttctggcgcc cgcgtgtgcc
    10321 cggcctgaag acgtccggct gccggcatcc ggctcgtggg cgggtgttgg ttcggccttt
    10381 gcggcgtatc ccgaccgcct gttgcccgga ctgggcgacc gcctcctcgc ggtggacgcg
    10441 gcggtgcagc cgcgggccgg cgccgtggcg cggctggccg ccgtccgtct ggcgcggggt
    10501 gagggcctcg atgccacgct ggcggcgccg ctttacgtgc gtgacaaggt ggccctgacc
    10561 accgccgagc gtctggccca gggcgggcgg gcgtgagcgc cgaaaccgtc gccttcgtgc
    10621 cgatggccga aaccgacctc tcctgggtcg cggaacagga tcgcgtcctg tatcccttcc
    10681 cgtgggtcga acagaatttc tcggactcca tggccgccgg ctacagctgc tggctgatgc
    10741 gggccggcga cgcgccggtc ggctacgcag tgctgatgag ggtgctcgac gaggcgcata
    10801 tcctgaacat cagcgtggct gcggactgtc aggggcgggg attcgggcgg cgcctcctcg
    10861 ggcatctgtg cgcagtggcg cttgcggcgg gggggcggca gatgtttctc gaggtgcggc
    10921 cgtccaatgc gtcggcgctg gccctgtacg cccgcgcagg atttgagacc atcgggcgcc
    10981 gcaagggcta ttacccggcc ccggaggggc gcgaggatgc gctggtgatg aggttgcctc
    11041 tgtgagcggc cggcgcgaca gcgtgttgcg ggaaatgggc ctggcgcccc tgtggcgctt
    11101 gcgggaccgt ccggccgtgg acgacgcctg tggccagctc gcggacgcgg tcgtcgacag
    11161 agcgccgctt caggccgagc gctcggcgga taccgtcccc gcggctgccg gcgcggtcga
    11221 cctgcggatc gtgccaccgc tgcattccga tgcccggcgg ggccacgccg ggctgccgcc
    11281 ccttgcggcc ggcgtggcgc ccgcggtgac gccggagcac gtggccgacg gtcgaatcga
    11341 agggctggac tgggacgcgc tggaggcgac catccggacc tgcgaggcct gcgtcctgtg
    11401 ccagaagcgc aagcaggccg tgcccggcgt gggcgaccgt caggcggact ggctgttcgt
    11461 cggcgaagga ccgggtgcag aggaagacga gcggggcgag ccctttgtcg gtcaggcggg
    11521 caggctgctg gatgcgatgc tggcaagcat cgacctgagg cgcggtcacg atgtgtacat
    11581 cgccaatgcg gtcaaatgtc gtccgcccca caaccggacg ccggagacgg ccgaaatcgc
    11641 ggcctgtttt ccttacctgg agcgccagat cgcgctgctc cagccacgcc tgatcgtggc
    11701 cctcggccgg ccggcggccc aggcgctgct gaaccaggag atcaagatcg gtgcttcgcg
    11761 ggggcggctc ttccggtacg gggggatccc ggtcatcgtc acgtatcacc cggcctacct
    11821 gctgcgcaat cttcacgaca agggcaaggt atgggaagac ctgtgctttg cccgccggac
    11881 gatggccgag ctgcacgccc gcgccgacgc aggggcgccg gaaaacctgc cgaccatctg
    11941 atttaactgc cttcctgagg gatgaaccat gcgtttcaag ctcttgctgt cgatgctcct
    12001 gtccgtgctg ctgctctcgg gctgcggcta caaccagatc caggtcaatg acgagcaggt
    12061 caatgcggcc tggtccgagg tgctcaacca gtacaagcgc cgggccgacc tgattcccaa
    12121 cctggtacag gtggtgcagg gctacgcgga gcacgagaag gacgtgctga tcaaggtgac
    12181 cgaggcccgc gccagcgtgg caggcgtcaa ggccacgccg gagctggtga acgatccgga
    12241 ggcgatggcg cgcttccaga aggcccaggg tgacctgtcc ggcgccctgt cacgcctgct
    12301 ggtggtctcc gagaactatc cgaacctcaa ggccgacgcg aatttccgcg atctccaggc
    12361 ccagctggaa ggcacggaaa accgcatcac cgtggcccgc aaccgctaca tcaaggcggt
    12421 gcaggattac aacgtgtcgg tgcgcacctt cccgaacaat ctgaccgcca tggtggtggg
    12481 tgccaagccc aaggccaatt tcgccgtgga caacgaacag gcgatctccg aggcgccgaa
    12541 ggtcaatttc ggcgcgccgg ccaagccggc ggcccagtga cagtgcgtcg tcccttcgac
    12601 ttcccggggc ccgcggtgtg aacctccggg cgcgcctgct ggccgtgtgc gtcttcatct
    12661 gtgccttcgt cgccggggca tgggcggagg atctgaagcc catcccgccg cttgcggcgc
    12721 gcatcaccga caccgtcggc ctgctgcctg gggcgaatcg ggcagcactg gaggcccggc
    12781 tggccgatct ggaggcccgc aagggtgccc aggtggccgt gctgatcgtg gccagcaccc
    12841 agccggagga catcgccgcc tattcgatcc gcgtcgtcga ggcctggaag ctgggccgga
    12901 ccggggtgga cgacggggtg ctgttcctga tcgcccggga cgaccggcgc atgcgcatcg
    12961 aggtgggacg cggcctggaa ggcgcgattc cggatgcggt ggccaaacgc atcgtcgccg
    13021 agcgggtggc gccccgtttc aaggccgggg attttcccgg cggcatcgag gcgggtctcg
    13081 aggccctggt cacgcagatc gagggggaag aggcattgcc gccgccggca ccggctgcca
    13141 cgcagggcaa cgatcttgag gattttcaag gccctgcatt catcggctgc gtcgtgtttg
    13201 ccttcatcgg cggtgcgatc ctgaaggcga tcttcggccg gctgattggc gcgaccctga
    13261 ccggaggtgt gatcgctgcg gtgatctggg ccgtgaccgc ggcactggtc ttcggcctga
    13321 tcgccggtgc gattgggttc atcttcacgc tgggtgcggg gggcgcacgc ggacgccatg
    13381 tgggcggctc ctcgtggagc ggtggtggct tcggtggcgc cggaggcttt ggcggcggga
    13441 gcagcgacag cagcgactcc ggtgggggat ggtccggtgg cggcggcgat ttcggcggtg
    13501 gcggcgcctc gggagactgg tgatggagtg gcgcaggatt ctccggcatc tcgccgttcc
    13561 gcactggacg gtgcgtcgcg cgttcacgtc gacggtgctt tccgccatcg agacggcgat
    13621 ccggcgcagc gaggcgaccc accggggcga gatccgcttt gcagtggagg gcggtctggc
    13681 gtggtccgat ctgcgccgcg gactcaccgc acgacagcgg gccgccaagg tcttctcgga
    13741 tctgggtgta tgggataccg ccggcaacac gggcgtgctg atctacgtgc agtgggtgga
    13801 ccggcgggtg gagatcctcg cggatcgagg cattgcggcc tgtgtgccgc aagccgagtg
    13861 gaacgcggtg tgtcacgggc tggagcaggc ctttaaggcg ggccgctacg cggagggctg
    13921 caccgcggcg gtggacgccg tcgggcagct gctggccacc cattttccgg cgggggcgga
    13981 caatccggac gagttgccgg atcgcccggt cctcctctga cagcggtcct cagctttggc
    14041 cgatgctgaa gtagagggcg gcacccttgc ccacctcgcc ttcggcccat acctgcccgc
    14101 cgtgacggcg cacgatgcgg gcgacggtgg ccagaccgat gccggtgccg gggaattccc
    14161 ggtcggaatg caggcgctgg aaggggcgga agaggttttc ccggtagcgc atgtcgaagc
    14221 cggcgccgtt gtcctgcacc acgaaggcgt ccatgccctc gtggcggacg gcggagaagc
    14281 ggatttccgg cacctcgacg cgtccggtga acttccaggc gttgcccagc aggttttcca
    14341 gcacggtgcg catgaggctg gggtcggcgt ccaccttgag gtccggggcg atctcgacga
    14401 tgacgtggcg gcccggttcc tgctggcgca gctcgtcgca gatggcctgg gcgagcagtg
    14461 acaggtccac cgggcggcgc tccagctcct tgcggctgac ccgtgacagg gtgagcaggt
    14521 cgtcgatgag ctggcccatg cgatgcacgg ccgcacgcac gcggcgcagg agctccttgg
    14581 cctcgtcgtc gaggcggtcg gcgtagtccg agccgaggat ggtgctgtag ccctcgatgg
    14641 cccgcagcgg cgcgcgcagg tcgtgggaga ccgaatagct gaaggattcc agttcggaca
    14701 gggcggcgct gagctgggac gtgcgctcat tgacgcgccg ttccagctcg gtgttgaggc
    14761 gcttgatggc gcgctcggca aacttgcgct cggtgaggtc ggtggccacg ccgatgatgt
    14821 ggggtacgcc gtcgaggatg acccggcggc ccaccaccag gaaaggaacc ggatccttgt
    14881 cgcgacggcg cagcagtact tcggccgagg cctgcccgga ggtgagggcg acctgcagac
    14941 ggtgttccag gatgggttcc tgttccggga ccaccagttt ttccacttcc gcatggtgca
    15001 gctcgtcgtc cgacaggccg gtaagatcct tgacgaagct gttccacagc accagccggc
    15061 cggcctggtc gaacaggaag aagggcgccg gcaggctgtc gatgaggctg cgggagaagt
    15121 cgcgctcggc cttgagctgt tcctcggcct gcttgcgggc ggtgatgtcg ccgatggagc
    15181 cgaccacccg taccgggcgg ccgcggcggt tgcggtgggc caggccgcgg gcgtgcaccc
    15241 agtggtagcg cccgtccttg tcggcgatgc ggtattcgga ttcgaagtgt tcggtgcggc
    15301 cgcgcaggtg ggcgatgaag tcggcccgca cgtggggacg gtcgtccggg tggatgagct
    15361 tctgccattc ctgaccgctg gtgcgcaggg tgtcgctggt gaggcccagc agggacagca
    15421 ggcgggccga caggtagaga tggcgcgtgc gcggatccca gtcccacagg ccttcgttgg
    15481 tgccgcgctg ggccaggacg tagcgctcgc gggtgtcgcg cagctgggct tcggtctcga
    15541 ggaaggcggt gcggtcctcc acggtgccga tgccgccgat cacctcgccg tcggcgtcga
    15601 gttcgggcgt gacgcggatc gacaggttga gcggggcggg tagcatggtc gagcggaagg
    15661 ggccgtcata ggcgccgggc tggcctgaca gcacgtcccg cagggtggcg agcaccgtgt
    15721 cgtcttcaag cccgtgcaga tccaggccga ccagctggtc cggcgtggac cggaacaggg
    15781 tcgccagccg ggtattgcag tacacgatgc gcaggtcgcg ggagaatcgc aggatgccga
    15841 cgggggaatt ctccaccagg gcgaggtagc gggcccggtc gccggcagcg ctgtcccgga
    15901 cggttcgttc gtccgtcagg tcgtgcagga cgatcacctc gcccaggggc agatcgctgg
    15961 cgctgccccg gatcggtgtc cggcagcagg caaccgtccg gacctcaccg acccgatcca
    16021 ccaggcggat ctgacggggc aggccgcggt cggatgcggg gccgtccgtg gggggctcca
    16081 ggtgcagaac gacgcgcagg ggcttgccca cggcgtcctg ttcggtccag ccggtcagca
    16141 cgcaggccgc ggtgttcatc aatgtgatgt tgccggcggc gttcgttgcg atgacgccat
    16201 cgccgatgct gcgcagggtg acgcccagat attcgcctgc ctgctgcgcc gcagtgtggt
    16261 cggacgccgc gccggcaatg gcccgcaggc tgccgtccgg gttgcgcagc aaggtcatgg
    16321 cgtcctgaat gtgcaggagc gcgccgcctg cagtgtgcag acggtaggtg accgtgagat
    16381 ccaccgggcc cgtgccgggc tgggccaacg ccgcgtcgat gcgggcccac agcgcgccca
    16441 ggtcgtcatc cggcacgcgt tctgcgaaat gcttccagcc gcccggctgg ccaagggcgt
    16501 cggcagagac ccccagccac tcggtcatgc gggccgatac gtagtcgtaa tgttcgtggg
    16561 cggcgccgta gcgatagagg gcgagtggca ccttgtcgag gatgtcggca atggacggct
    16621 cgaggcggtt cggcagcgcc cgcgagatgg cggcttcctg gacgtagacg acccggcagg
    16681 gatgtcccgc gaagacctga tcgtaaaggg tcgtgtcgag ggcccggatc gtgccgtcgg
    16741 ccaggcgatg ccccagcagg caggggcccg gattccactc ggccgacgtg ctgtccgcca
    16801 gcaccgtggc cacgtccgcg ccctccatct gggacgcgag acggccatag agccgcccgg
    16861 cggccgggtt ggccgcaacg atccggcggc tgcgtgtatc gaaaaaccac actgcatccg
    16921 ggacgaactc gaagaaagca cggtaggctt gtgccgtggc ttcacaggcc gcagcccgtt
    16981 cggtcacctg ggcgaggaag tgacggatgc ccaggtagag gccgagcgcc gtgacgccga
    17041 cgagcagcag tccgtcctga atcagcacgc cggcggcgcc ctccgccgtg tgttcggtca
    17101 gcaacaacca ggccgtggcg gtgaaggcat agagcccggc cgccaccagg gcgcagcgtt
    17161 ccggctgcgc aggccgaacg cccgcgccgg tcatcgtgcc ggaagacgcc gggacaggag
    17221 gcagcgtcat ctgagccgtc ctttaaggcc atgcgggagg gtgggggccg gtgcttccac
    17281 gttgctgaga taaaatccgc cgattgcttt acgactattc actgttccaa tgaccgagtc
    17341 cgacctcgat cgggataccg cctacatgcg ccaggccatc gaactcgccc gcgaggccgg
    17401 ccgccatggg gaagtccccg taggcgcgct ggtggtcggg gacggaggga tcgtcgggcg
    17461 cggcttcaac cgcccgatcg gcacccacga cccgacggcc cacgccgaga tcatggccct
    17521 gcgggacgct tctgcccgcc tggcgaacta ccgcattccc ggccataccc tgtacgtcac
    17581 gctggagcct tgtgccatgt gcgcgggcgc catcctgcac gcgcgcatcg cacgggtggt
    17641 attcggtgca acggacccga agacgggtgt ttcaggtagc gtactcgatc ttttttccga
    17701 gaaacgccta aaccatcaca ccaccgtcgt cggcggcatt cttgcagagg aatgtggagg
    17761 gcttttacgg gattttttcg cgatgcgccg aaataagtcg gtatcagggg cataaatttt
    17821 gcaaattctc attgatatcc ccagccaatg gctgtccctc ctggccgacg atggcacctg
    17881 cctgcggcgc taccgcgttt cgacggcccg caacgggccc ggcgagcgct cgggcagcca
    17941 ctgtacgccc cgcggccggc atgtgatccg cgcccggatc ggcgcgggcc agccccttgg
    18001 cgcagccttc cgcgggcggc gtcccaccgg cgaggtgtgg tcgccggaaa tggccgcggc
    18061 gcagccggag cgggactgga tcctcacgcg catcctgtgg ctgtcgggcc gggagccggg
    18121 gcgcaaccgt tggggcgacg tggattccat gcgccgttac atctacatcc acggcacgcc
    18181 ggacaccgag cccatgggcg tgcccctgtc cattggctgt gtgcgcatgc gcaaccggga
    18241 cgtggtggaa ctcttcgatc tggtgccggc cggcacgccg gtggagattc gctcgtgagc
    18301 ccggcccagg acgccgtgcc ggaggtcatc ctcggggcgt gggacgtcct gcgggacgcg
    18361 gcctgtcaca tccgcttcgc ggtgttcgtt gatgaacaag gggtgccggc ggagatggag
    18421 gtcgatgagg ccgatccgtc gtcctgccat gccctggcgt ggatcggcgg cgcagcggtg
    18481 ggcaccggtc gcctgttgcc ggacgggcac gtgggacgca tggcggtgct gccggcgtgg
    18541 cgcggacggg gcgttggccg tgccgtgctg acggcgttga tggccgaagc ccgccggcgc
    18601 ggtctgtcac ggctggtgtt gcacgcccag acccacgcag aaggcttcta cgcgggcttc
    18661 ggcttcgtgg cggagggcga ggtcttcgac gatgcgggtc tgccccatcg gaccatggtg
    18721 tggcaggtgc aggggtgatc ccttcgcgct tcaggcaacg ggcctgcgcg ggtcgcccac
    18781 cccgtcccgg agtgccttga gcacagtgtc cgaaggcgcc gtcaggcggt catggcgggc
    18841 gcctgcccgg cggggggcgg caggtggcgc aggaaccagt cggcggcgtg gctggcggcc
    18901 tcgtcgaggg tgcccggttc gcggaacagg ccgtcggtgc cgggtatctc gcgccaggcc
    18961 ttggggccgc ccagcagttc ataggcctgc cgcacgccgg cgatgccttc gtcccgctcg
    19021 ccgacgatca tgcaggcagg gctctgcacc aggcgcaggg ggtcggcgcc cgccagtccg
    19081 gggcgtccgc cgcggcacac cagggcggcg atgaattccg gctcgcggct catggcgcgg
    19141 atggcggcgg cgcagccgtt gccgctggtg accagcccga cggcgagtcc ttccagcggc
    19201 ggctgatggc ggatccattc gaagagggcg ttgaggcgtt ggcccagcag gggggcgttg
    19261 taacgggtgt ccggatcgcg ggtctcctcg tagcgggtga ggaggtcgaa gatcagggtg
    19321 gcaaagccgg cggcttcgat gcgggcggcg aagtgcgcct cgcgggagtc ctgatgatgg
    19381 ccggcggcgt tctgggcgat caccaccagg ccgcgggcat ccggggcatg ggcgagcagg
    19441 ccgtcgagcc agacggggcc ggcggcgatg ctgaggggcg tgtggcggag tttcatggtc
    19501 ggattcatcg cttgcacgat ggcacgggcc ggcaggcatc gcaagccggc ggcgcccgct
    19561 tcaggggcct gcgtgccggt ggatgtgcac tgggacacgg cgcggcccga aggcgccggt
    19621 gaagtcgccg aaatgcccgg cgatcacgtg tccgcaggcc gtgcaggcac catgggcatc
    19681 aaggcggtag gcgaggatgc ggtagccgtc ccgctcgatg agcgtctgtc cgcaggcggg
    19741 gcagcgggtc gaagcgcctt caatgtcgcg tacgttgccc gtgtagacgt aggccaggcc
    19801 cgcgtccagc gcgatgcgga gggcgcggcg cagggtggcg ggcggggtcg gcggcagggt
    19861 gtcgagcttg tagtcggggt ggaaggcggt gaagtgcagc ggcacgtcgg tccccagttc
    19921 gtcggccacc cagcgggaca gggcgctcag ctcggcgtcg gaatcgttga ggccggggat
    19981 cagcagggtg gtgatctcga cccacacgtc cgtctcgtgg cgcagataga ggagggtgtc
    20041 gaggaccggg gcgaggcggg cgccggtctg gtgcacgtag aagtcgtcgg tgaaggcctt
    20101 gagatccaca ttggcggcgt ccatcacggc gaagaagtcg cgccgcggcg caggtgagat
    20161 gtagccggcg gtgacggcca ccgtggcgag gccgcgggcg tgacaggcaa cagcggtgtc
    20221 gatggcgtac tcggcgaaga tcaccgggtc gttgtaggtg aaggcgacgc tgcggcagcg
    20281 gtgggctgcg gcggtggcgg cgatctcgtc cggcgcggcg gcgtccatca ggcggtccat
    20341 gtcccgactc ttggaaatgt cccagttctg gcagaacttg caggccaggt tgcagccggc
    20401 ggtgccgaag gagaagacgc tgctgccagg gtggaagtgg ttgaggggct tcttctcgat
    20461 ggggtcgatg cagaagcccg aactgcgccc gtaggtggtg agcaccaggc cgtcgcccac
    20521 gcgctggcgc acgaagcagg cgccgcgctg gcctgcgtgc aggcggcaat cccgcgggca
    20581 caggtcgcac tggatgcggc cgtcgtcgag ccggtgccag tagcgggcgg ggacgccgtt
    20641 cagggcgtcg ggacctccga ttccttccat ttttccacct cgtagcgggc gatcatcacg
    20701 ttcggccccc agaaatccac cggcaggcgg gccttctgct tgagggcggc gaggaaggcg
    20761 cggggctccg gcagctgcgc ccagacctgg ggcaggaagg tggccttctg gcagccattg
    20821 aagaagatca ccccgtcgcg gccggggacg agctgggaca gggcgtctgc ctcgtcgcgg
    20881 aattccagga attccggcgc ggtgaggacg gacacttcca cccggacggc gtcgaactcg
    20941 cgggcgtcca ggggcggaaa gcgggaatcc ttgagggccg ccgctgccgc gttggcgcgc
    21001 acatcctcac ccagagggcg gtgggcgctg aggctgccaa tgcagccgcg cagctcgccg
    21061 ttgcgggtca gcgtgacaaa gctggcacgt cgctcggaca ggcgcggatc ttctggtgcg
    21121 ggggcatggg gcaggccaag ggcgtcggca atggcggcac gggcgtgggc cagcagcacg
    21181 gggccgaggg cgtcagtggc aggcatggtc cagatcctcg gaaaaggcga tggccgcgta
    21241 gccaaccacc cggttgcggt cgcccgcggt gtcgccggag ttgcgctgat ccagcacctg
    21301 gggacgcagg tgatggcgca gggctgcgag cagcaggccg ttgatcggcc cggcgccgca
    21361 ggcctgctcg ggctcgatgc gcgggtcgag ctgctggatc tgggccaggg tggccttgtc
    21421 gcgcagctgg gcctggcggt aggggtggta gtgggagaga tcggaactga cgaggatcag
    21481 tgtctccggg ccgccccaca gggcctcgat ggcggccgcc acggaggcgg catcggcctg
    21541 acccaccgcg atgggcacga tggcgaaatg atccagcacg cattgcagga agggcagctg
    21601 cacctcgatg gcatgctcct gggcgtgggg gcggtcatct acgaccacgt cgggcagcgt
    21661 ctgggcgagg cgccaggcgt cctgatccac ggggaccgcg cccaagggtg tgatgaagga
    21721 caggctggcg ggcagcgcca tgccgcggat cgcaacacgg tgggtcggac agaggatcac
    21781 gacgcggcgc aggacgccac ggcggcgggc aagcgcttca taggcggtgg ccgccaccgc
    21841 accggaatag atataacccg cgtggggcac gacaagggcc ttgggacagt cgatgcgctc
    21901 ggccgggacc gcgccggcca gcaggccact gatctgggca tgcaggctgt gggggtcggc
    21961 cggatagaac agcccggcga ccgccggcgg acgttgggat gcgtgtgcca tgatgccttt
    22021 ctcccggtga ggggaccgaa tgtcacttcg tattataggg acgccagatg aagccacgcg
    22081 atgccgccac cgagtcccgc gccgacggcc gccatgccgg ccgccacgcc gctgcccagg
    22141 tgccgtccgc ccaggctcag ggcgaggccg ctcttgaagc tcaggttcgc cagcatggcc
    22201 aacaggatgc cggtggcggc ggggtcggcg cccagcttgc cggtgttgag caggcgcagc
    22261 atcgaaaggg tgatggcatc cacatcggtg agcccggagg ccagggccag catgtagagg
    22321 ccgccctggc cggcgatgtc cgacagccag gccgcgcaca acaggaccag ggcgtagagc
    22381 aggccgaagc ccatggcggc ccgcagttcc gtcgggttgc gggtgtccgg gatgggcagt
    22441 tcgccctgat ggacgagttg acgccagttc caggccagca cggcggcacc tgccaggagc
    22501 gccagaccgg cacccggcca caggcgcggc gccaccgacg gggccacggc gagggcgatg
    22561 ccccccacgc gtacgaccat taccagattg gcgagcagga tcaccagttt ggccgtggac
    22621 accatggccg gattctcgcg ggcatggcgg gagaacacca tggttgtcgc ggtgctcgac
    22681 accagtccgc cgagcaggcc cagcagcggc gcaccgaggc gcgcgccggc cagacgcagg
    22741 gcggcgtagc cggccagact cacgccggcg atgagcacga ccatcaacca gatctggtgc
    22801 gggttgaggg cctcgtaggg gccgaagccc cggtccggca ggatcggcag gacgatcagc
    22861 gagagcaccg cgaattgcag gatcgaacgc caatcctggg ccgtgaggcg ggccgccacg
    22921 ccgctcagtt ctgatttgaa gtagagcagc atcgtcgcgc caaccgcggc catcaccgca
    22981 atgcgaccga agcccagcca caccattgcg cccagggcga agcacaccag gatggcgatg
    23041 accgaggtgg taccgggatc gtgggggtcg ggcctgcgca gataggcggc gatcatcatc
    23101 accgccacca ggaccagacc cgccaccagc acgcccggcg tgccgagccg ctccgccagc
    23161 agggcgcaga gggcgccgag cagtccggtg agcccgaagg tgcgcacgcc ggcgcgggag
    23221 gcgggatggc gctcccgctc caggcccatg agcagaccga tggcggtggc gaccgcgagg
    23281 gcttccagct gggcgggatc gaaggcctga tggaagagca gggtgttcat ggcgtcagcg
    23341 ccggagggcc cccgcgcccc gaaaacggcg gccgacgggg acattgagta gaatccgccc
    23401 catgcacacc catcacagcc gatgttttgc cctcgtgcct gccgccggaa gcggcagccg
    23461 catgaagagc gaacagccca aacaatatct ttccctcctc ggcgaacccc tgatccgcca
    23521 caccctggcg gtattgtgcg cctctccgct gctggagcgg gtgtacgtgg tgctgtcggt
    23581 gggcgatcag gagtgggacc gccacccctg gcattcgttc ggcgacaagc tggtgccgct
    23641 cttttgtggt ggggcctcgc gggccgacag cgtcctcaac ggcctgcgcg ccatcgccga
    23701 cgacaccgca tcctcggact ggatcctcgt ccacgatgcg gcgcggccct gcctcgcgcc
    23761 gtggcacatt gaaagcctgg tgcgtgagct gatccacgac gaagtgggcg gcattcttgc
    23821 tgtacctgtg gccgacaccc tcaagcgcgc cgacagccag cagcgcatcg ccgccacggt
    23881 gccgcgggac ggcctgtggc aggcccagac gccccagatg ttccgctacg tcatgctcaa
    23941 gcgtgccctt gaagccgccc gcgacgtgac cgacgaggcc agcgccatcg aggccatggg
    24001 tctgcggccg cggctcgtgc gtggcgatcc caccaatctc aaagtgacct atcccctcga
    24061 cctgcatctg gccgagtgga tcctgcaaca tcgcgaagga ctcgcatgat cgttccgttc
    24121 cgcatcggcc agggcttcga cgtgcacgcc ctggtgcccg gccgcgccct cattctcggc
    24181 ggcgtcacca ttccccacac ccacggcctg ctgggtcact cggacgccga cgtgctgctg
    24241 cacgccctca ccgacgcgat cctcggcgcg gcggggctgg gagacatcgg ccgccatttc
    24301 cccgacaccg acccgcgctt cgagggcgcc gattcccgcg tgctgctgcg cgaggccgcc
    24361 gccgcggtgc gccgcgccgg ctggcagacg gtgaatgtgg acgccaccgt gattgcccgc
    24421 gcgccgaaga tcctgcccca cgtgccggcc atggtggcgt gtatcgccgc cgacctggag
    24481 gtggcgccgg aggccgtcaa tatcaagggc aagaccaccg agaagctggg cttcaccggt
    24541 cgcggtgagg gtatcgcggc ccaggcggtc gcgctgctgg cgcgcgtgga gacctgaggc
    24601 gcggacg
//
LOCUS       DAOQMM010000048        24085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_670_length_24085_cov_46.592468, whole genome shotgun sequence.
ACCESSION   DAOQMM010000048 DAOQMM010000000
VERSION     DAOQMM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24085
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_670_length_24085_cov_46.592468"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLH85_09550"
     CDS             complement(<1..802)
                     /locus_tag="PLH85_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNB64849.1"
                     /translation="MTPQDVMKMVQENEVKFVDFRFTDTKGKEQHVGVPLSAFDEDKF
                     ESGHAFDGSSIAGWKGIQASDMLLMPDPNTAYIDPFFDETTLVLTCDLIDPADGKGYD
                     RDPRSIANRAEAYLKASGIGDTAFFGPEPEFFIFDSVEWKVDMSGAYHKIFSEEGAWS
                     SDIKYEGGNTGYRPAVKGGYFPVPPVDSFNDIRAAMCLALEACGVPVEVHHHEVANAG
                     QNEIGTKFNTLVRRADQTQILKYIVQNVAHQYGKTATFMPKPIVGDNGS"
     gene            complement(998..2176)
                     /locus_tag="PLH85_09555"
     CDS             complement(998..2176)
                     /locus_tag="PLH85_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB64850.1"
                     /translation="MAHDSPLSAATEAPLDADRARQAQQATWVSIAVNVVLTVLQIAV
                     GLFAHAQSLVADGMHTLSDLLGDFMVMVASRRGSTPADERHPYGHGRYETAASMILGL
                     VLAGVGAGFLLSAASRLQSTEALPALHPAALWMALLTLAGKEGLFRYMLAVAERLRSP
                     MLVANAWHARADAASSLVVAVGIGGSLLGYRFLEPLAAALVGFMIIRMGLQLSFDALR
                     ELIDTGVAPEEVASIRRAVEETPGVLGVHDLRTRRMAHRILVDAHVRVDARISVSEGH
                     RIAELARHRVRSRVEDVLDVLVHVDTEEDEELAAGRHLPSRDVLLAEIAEILGPDLPQ
                     TERIILHYVGKQVEADVFLPHVWCCDARRIDALRQQVADGVARRPHWGRIALLCQIAP
                     "
     gene            2240..2692
                     /locus_tag="PLH85_09560"
     CDS             2240..2692
                     /locus_tag="PLH85_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB64851.1"
                     /translation="MGKLSETLALAQQRSRDAGLPYAGLLTPREAWDLLQAAPGARLV
                     DVRTRAEWDWVGRVPGAVELEWMLYPGNEPNPHFLAQLQRQVDPEALVMFLCRSGVRS
                     NNAATLAAGHGYTNAYNILEGFEGDKDAHGHRGKLGGWRFAGLPWIQG"
     gene            2719..3819
                     /gene="nadA"
                     /locus_tag="PLH85_09565"
     CDS             2719..3819
                     /gene="nadA"
                     /locus_tag="PLH85_09565"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNB64852.1"
                     /translation="MQTASISFDRFNALADADAHARIRAARARLGDKAVLLCHHYQRA
                     DVYQYADITGDSLKLSRLASDTDAEYIVFCGVHFMAEVADIMSRPQQIAILPDLAAGC
                     SMADMANLAKVERCWRELTEVLDTPDALITPVTYINSAADLKAFCGEHGGIVCTSTNA
                     PVILDWAFAQREKVLFFPDQHLGRWTGFNKGIPLDEMVVWDPDLEYGGLTAEQIRKAR
                     ILLWKGHCSVHQMFQPAHILKFRNQHPDGKVISHPESALEVCRQSDFVGSTEYIIKVV
                     KESAPGTRWLVGTELNLVNRLAEEVKHEGKIVQFMAPTVCMCSTMQRIDPQHLAWTLE
                     NLAEGNVVNRITVPAHEAALAKVALERMLAVS"
     gene            3901..4650
                     /locus_tag="PLH85_09570"
     CDS             3901..4650
                     /locus_tag="PLH85_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB64853.1"
                     /translation="MSTLRICTYNIHKGFSQFNRRLAIHDVRERLHQLGADVVFLQEV
                     QGQHLHHARRHTDWPEQPQHEFLAGDLWRDTAYGGNALYDHGHHGNAILSRHPILSRA
                     NQDVSDHRFERRGLLHCEIRLSGVARPVHCVCVHLGLTAGTRRRQMAALVARLNVLAP
                     PTEPLIIAGDFNDWRNHADDHLIRQLGVVEVFTALRGRPARSFPCHLPMLRLDRIYVR
                     GFAIDQAEVHFGAPWNRLSDHAPLTAQLRAH"
     gene            4660..5820
                     /gene="clsB"
                     /locus_tag="PLH85_09575"
     CDS             4660..5820
                     /gene="clsB"
                     /locus_tag="PLH85_09575"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HNB64854.1"
                     /translation="MAIHWLPGNALTLLENGAEFFPALQAAIDTACHEVYLETYIFAD
                     DVTGRAIAAALARAAARGVTVRLLVDGFGGREFVERLRDDLIARGVHVLIYRRDISPF
                     AIRRRRLRRLHRKLAVIDGRVAFVGGINILSDLSEPNMAAPRHDYAVRVEGPLLAPIH
                     ATVQHLWELVAWASFRHRHRLPAGPAPCCGTCGDVEAAFLIRDNLRHRRDIEDAYLLG
                     IAAARREIVLANAYFLPGRRFRQALLDAARRGVRITLLIQGLADHPLQQRATRALYGQ
                     LLAGGLRIVEYHRSYLHAKVAVVDERWATVGSSNIDPFSLLLAREANVVIRDAAFATH
                     LRDHLQDAIAHGGREISADAWQRGPRLQRLLNWAAYGLVRLMIGLAGYGGRH"
     gene            5882..6370
                     /gene="rfaE2"
                     /locus_tag="PLH85_09580"
     CDS             5882..6370
                     /gene="rfaE2"
                     /locus_tag="PLH85_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNB64855.1"
                     /translation="MNYPAPAFEAKICHPQDLAARVAALPRPLVFTNGCFDILHRGHV
                     TYLAQARALGAAMVVALNSDASVRRQGKGDDRPINVLEDRLAVMAALDCVDLVTWFDE
                     DTPIARILDCQPDVLVKGGDWPVERIVGAPETFARGGHVHSIPFEHERSTTALLTKIR
                     RL"
     gene            6481..7413
                     /gene="cysK"
                     /locus_tag="PLH85_09585"
     CDS             6481..7413
                     /gene="cysK"
                     /locus_tag="PLH85_09585"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNB64856.1"
                     /translation="MRIASNVAELIGKTPLVRLNALAPANGATLALKLEFFNPAHSVK
                     DRIAAAMLDAAEAAGKIKPDTIVLEPTSGNTGIGLAMVCAARGYKCAFVMPETMSRER
                     KLILKAYGAELILTPGAEGMPGAVKRATELAAADPRYFIPQQFENPANPAIHRATTAE
                     EIWADTDGQVDIFVSGIGTGGTITGVGEVLKERRPGVKVIAVEPAASPVLSGGARGPH
                     PIQGIGAGFVPAILNTAVYDEIVQVPNDAALDTARRLASQEGLLVGISSGAAVWAALQ
                     VANRPENAGKLVVVVIPSFGERYLSTVLYQHLEV"
     gene            7500..8660
                     /locus_tag="PLH85_09590"
     CDS             7500..8660
                     /locus_tag="PLH85_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNB64857.1"
                     /translation="MTLPFDFAHGAERAGSDSIKWRRYAGRDVIPMWVADMDFAAPPP
                     VLDALRARLDHGVFGYADPWPGVLEAVIDGIARDHGWRIQPEWLVWLPGVVTGFNVAV
                     RTVGRPGDGVFTATPVYPPFLHAPGNFEQRLVTTPLLRTGTRWEWDWDRSAAALADGV
                     RLFMLCHPHNPVGRVFDRDELGRIAELAERHDLVVCSDEIHCGLVLNPTKAHVPLAAL
                     DERIGRRTITLMAPSKTWNIPALYCAFAIIPDGTLRARYKHAMAGIVPHVNVLGLVAT
                     EAAYRDGGPWREALLATLRSNAARVTDAIDLLPGLATTPVEATYLAWIDCRDFLAARG
                     LTGSPAAFFEQAGVGLSDGRDFGLPGFVRLNFGCPPARLEAALERMRRAVTA"
     gene            8660..9910
                     /locus_tag="PLH85_09595"
     CDS             8660..9910
                     /locus_tag="PLH85_09595"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB64858.1"
                     /translation="MGASPASFGLRTAPRDVRLYVVTTVCSALIHFGQGVAVGWSVYE
                     QTGAPLALGFVGLAQFLPIAVLFLPAGHLADRVERRRMVLVSLLLTFAATLLLAYAAW
                     RALPVGWTYAALVLSGCGQILYRPARDALLPALLPPQHLARGIALASALYQVASLAAP
                     AAVGFGLAQVHSAVPIHLCNALLAALALLATIAIRQRSHGQPAATGSRVAEVLAGFRH
                     VWRTRILLAVMSVDLVAVLFGGAVALLPVYARDILHVGPAGLGLLSTAPALGAACAGL
                     VMTRSGVALDGRVLLAAVGGFGVATVVFGLSTAFPLSLAALAVAGGCDCVSVVLRQTV
                     LQQATPDALRGRVGAVNRVFVSSSNELGALESGLAAAWFGPLLAVAGGGAITVLVALG
                     APALFPVLRALQHDRPLPRDPAVS"
     gene            9977..11788
                     /locus_tag="PLH85_09600"
     CDS             9977..11788
                     /locus_tag="PLH85_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNB64859.1"
                     /translation="MTPLPSAPADALDGILQRHRHDATRLLQILIDAQDLDGWLPPPM
                     LTRIAAALDLPRARVEGVAGFYSFLYTQPVGRYRVLFSDNITDRMLGNAGLMDALCAK
                     LWVEPGKRSEDGLVSIATTSCTGLCDQGPALLVNGRAIGQLDHRRINAIAELIRQQTP
                     LERWPAEFFRVTHNIRRRDALLNLDMAPGAALSAVLALGREGWLTEMEASRLRGRGGA
                     GFTTSVKWLSARNAPGAEKVIVCNADEGEPGTFKDRVLLAGFADLVFEGMTLAAWAVG
                     ARQGLLYLRGEYRWLRPHLDAVLARRRSAGLLGPAILGERGFDFDIDIHLGAGAYVCG
                     EETALIESLEGKRGTPRIRPPFPVTHGYLGRPTVVNNVETLAKTCLIALQGGAAFAAT
                     GTAQSAGTKLLSVSGDCAAPGIYEYPFGVTLARVLEDCGASDTQAVQVGGAAGLCLAG
                     YEFQRRIAYEDVPTAGAFMVFDATRDLFEVARAFVHFFAHESCGFCTPCRVGTALLKG
                     FMDKLADGHGAQADLADIEWLDRLLKNASHCGLGSAAPNPVIDTLQKFRPAYERRLLS
                     LDFEPAFDLDGALAEARRLTARNDPGAHLATHEETRE"
     gene            11785..12483
                     /locus_tag="PLH85_09605"
     CDS             11785..12483
                     /locus_tag="PLH85_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB64860.1"
                     /translation="MSKTFLLDGRPVPFEDGQSILQAARAAGQFIPSLCWHKDFPAHG
                     SCKLCIVKAAGRHVSACATPAREGWEVESNTPEMNNERRALLQMLFVEGNHFCPSCEK
                     SGHCQLQALAYEYEMLTPRFRHFYPERPVDASHPDMLLDFNRCIFCELCVRASRDVDG
                     KSVFALTGRGIAKHLTVNAESGRLADTDFAATDLAARICPVGVILPKGRGFAVPIGAR
                     PYDTGTIRDTAQED"
     gene            12634..13179
                     /locus_tag="PLH85_09610"
     CDS             12634..13179
                     /locus_tag="PLH85_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNB64861.1"
                     /translation="MTAPRKIRVATASLAGCFGCHMAFLDIDEHLLELAGQVEFDRSP
                     LTDIKDVGPCDVGLIEGGVCNAENVHVLRAFRRQCKTLVAMGACAINGGLPAQRNHLS
                     VADCLREVYQFRPGLADGRIPDDPELPLPLDKVRPLHEVVRVDYFLPGCPPSGAAVWK
                     FLSDLLAGRTPRLTGALLHYD"
     gene            13189..14652
                     /locus_tag="PLH85_09615"
     CDS             13189..14652
                     /locus_tag="PLH85_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNB64862.1"
                     /translation="MSLELETAADRAHLRRVAIDPVSRVEGHGKVTLLLDEGGHVQEA
                     RLHIVEFRGFEKFIQGRPFWEVPVMVQRLCGICPVSHHLAAAKALDTVVGATITPTAE
                     KIRRLMHYGQILQSHALHFFHLASPDLLFGFDAPAQQRNIIAVAAAHPDIATHGILLR
                     KFGQEVIRITAGKRIHGTGAIPGGVNKSLSPAERDELLRDVDTMIDWSRDAVALARRL
                     HESDADLYDHFGEVPGQFLSLVRPDGALDLYDGVLRLRGADGRVVRDGIPCTDYLGHI
                     TEAVRPWSYMKFPYITTQGPDTGWYKVGPLARVQNCDLIPTPRAEAARQAFLAHGGGA
                     PLHGPLAFHWARMIEMLHAAEVIRELLDDPDLLSDDRMSGGPRRREGIGIIEAPRGTL
                     IHHYQVDDDDLVSDCNLIVSTTHNNQAMNTAVRAVARRYLDGQTLTEGLLNHIEVAIR
                     AFDPCLSCATHALGKMPLALELRDMAGVLVDRLQRDT"
     gene            14658..15143
                     /locus_tag="PLH85_09620"
     CDS             14658..15143
                     /locus_tag="PLH85_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNB64863.1"
                     /translation="MGVVILAAGNPSRGDDALGPLLLAELAALAPDGVTLVEDFQFQI
                     EHALDLVGQDIALFIDAAASGAAPFCFAEIRPARSRAPSSHALSPDAVLQVYAETLGV
                     PPPAFVLAVRGASFALGAPLSSVAQAHLEAARPLLAACLAHPDVDAWRRLVHPRPSAA
                     D"
     gene            complement(15145..15702)
                     /locus_tag="PLH85_09625"
     CDS             complement(15145..15702)
                     /locus_tag="PLH85_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64864.1"
                     /translation="MLEIRREAGALDGTQWHAAGAPSGYMTDPVSDAALTGAAFVRHL
                     GDARFASLRALRQLLRALDAERVAIGVLDGSGTRLHEELVWSSGFGRCGESERDMALS
                     RFADDLAVWQHGTALSLLVDIDVSSEAWQDVPCNQLLLVRGPNHGAGCPVFVAQLRKT
                     LNLPEGAAHAISLLLNEYSQRRVAA"
     assembly_gap    15981..16080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16202..16822)
                     /locus_tag="PLH85_09630"
     CDS             complement(16202..16822)
                     /locus_tag="PLH85_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64865.1"
                     /translation="MSANTEVPEKEYCSTTEAAQRLGLSLGTVQQMVENGLLEGWKTA
                     GGHRRIRVSSIEDFRARAAAGGGQTVSRPPGVLQVLVAEDDGILQKLYEHTFSSWGLA
                     VQVRMVASGIDGLLEIGRMPPDLLITDLRMPGIDGFEMIRRLRADPQLADLDIVVVSG
                     LTDEEIAENGGLPADVTLYGKPVPFRELKGYVQAKIMAMQRNRKTA"
     gene            complement(16926..17447)
                     /gene="ssb"
                     /locus_tag="PLH85_09635"
     CDS             complement(16926..17447)
                     /gene="ssb"
                     /locus_tag="PLH85_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB64866.1"
                     /translation="MASVNKVILVGNLGKDPETRYAPSGDAICNITVATTDTWRDKAS
                     GEKREATEWHRVAFFGKLAEIAGQYLRKGSQVYIEGSLRTRKWQDKDGQDRYTTEIRA
                     DEMKMLGSRQGMGGGDAPARSEGGYGGGYGGAAPRETAFGGAPAAAPAPSAPPRKPAG
                     GAAFNDFDDDIPF"
     gene            complement(17515..18684)
                     /locus_tag="PLH85_09640"
     CDS             complement(17515..18684)
                     /locus_tag="PLH85_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB64867.1"
                     /translation="MSPAERRAGVSLAAIFALRMLGLFLVLPVFAVHAHGLPGGDNVG
                     LVGFAIGAYGLTQAFLQIPFGTASDRFGRKPVIVFGLLLFVAGSVVAALAQDVTTIAM
                     GRVLQGAGAISAAVTALAADLTRDQHRTKVMAMIGSSIGLVFALSMVAAPVLYGIIGM
                     SGLFWLTAVLAGGAILTVLFVVPAAPPVPRATGRFAEVLGNGQLMRLNFGVFALHLMQ
                     TAMWVLVPAGLVRAGLPVAEHWKIYLPAVLLSFAVMVPAIIMAERKGAMKPVFNTAIA
                     LLLVVQIGLYVEGSAGLWPLAILLTLFFVAFNVLEATQPSWISKIAPAHAKGTALGVY
                     NTLQSLGLFLGGALGGWLAQHEGPGAVSLLGGGLALVWLALAAGMTPPARRVVPA"
     gene            18775..21597
                     /gene="uvrA"
                     /locus_tag="PLH85_09645"
     CDS             18775..21597
                     /gene="uvrA"
                     /locus_tag="PLH85_09645"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB64868.1"
                     /translation="MDAIRIRGARTHNLKNVALDIPRDRLTVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARAGTPHCPDHPEHALEAQSVSQMVDAVLALPEDTRLMIVAPVISGRK
                     GEQADLFAELRAQGFVRVRVDGAVHELDSLPALDKNRRHSIEVVVDRFKVRQDLRGRI
                     AESFETALTHAEGRAIAVEMDSGTEHLFSARFACPVCSFALAELEPRLFSFNNPAGAC
                     PRCDGLGTVEFFDPARVVAHPELSLAAGAIRGWDRRNQFYFQLLASLASHYEFDIDTP
                     FESLPDALRQVVLHGSGRDKIAFRYLGDGGKAVLKEHAFEGIIPNLERRYRETDSLAV
                     REELAKYRATCACPACGGTRLRTEARHVLVGERNLSEVSRLPLAQCRAFFDTLALSGQ
                     RAQVAEKIVAEIAARLQFLINVGLDYLTLDRSADTLSGGEAQRIRLASQIGSGLTGVM
                     YVLDEPSIGLHQRDNDRLLDTLRRLRDMGNTVIVVEHDEDAIRMADYVVDMGPGAGEH
                     GGRIIAAGRPEDIAAHPESLTGAFLSGTRTIPVPAERRAPEPERLLRIEGASGNNLRD
                     VSLELPVGLFTCVTGVSGSGKSTLINDTLYAHVARHLNGSSMEAAPCTALDGLDHFDK
                     VINVDQSPIGRTPRSNPATYTGLLTPIRELFAGVPDAKARGYGPGRFSFNVKGGRCEA
                     CQGDGLIKVEMHFLPDMYVPCDICHGKRYNRETLEIRYKGKTIYDVLEMTVEQALDFF
                     APVPVVARKLDTLMQVGLGYIRLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDE
                     PTTGLHFADIELLLSVLHRLRDHGNTVVVIEHNLDVIKTADWLIDLGPEGGNGGGLIV
                     AAGTPEALAAHPASHTGRYLARLLKA"
     gene            21603..22514
                     /locus_tag="PLH85_09650"
     CDS             21603..22514
                     /locus_tag="PLH85_09650"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HNB64869.1"
                     /translation="MTDLWPRLAACLEHLAPAVPADALAEADTHRDTLAPRLVAVLDG
                     LVADPTPARADGYVLHLYAMLLLACWRDARAYRPLAALGHLDERIIDEVFGQLVHDTY
                     GRALASTCDGDLAPLTALADDPAASQWARAAALEALLVAALEGRAARTAVIAFLADFG
                     AREAQALRDNPADGEHFQLIDVVAVTAADLGAVELLPAIEAWYREGLIDSSFAEAADI
                     AADIARSPDDCRAALRHKGHGLIDDVAAEVALWPAFHSAPPVRGPALPLGLVREPFVR
                     DGVKVGRNDPCPCGSGRKYKKCHGAAV"
     gene            22609..23292
                     /locus_tag="PLH85_09655"
     CDS             22609..23292
                     /locus_tag="PLH85_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNB64870.1"
                     /translation="MTDTPATAENPAFDLDDFEVRVLAVLIEKSFVTPDIYPLSLNAL
                     LAGCNQLTGREPVMSLSETAVQDALDRLAERGLVSQRHQAGARVAKWEHQIRLRHSLT
                     PPMQAVLALLMLRGPQTPAEIRARCDRLHAFTSTAEVEEILEKLGDKFPPMVVQLPKA
                     PGTKEARFAHLLAGEGAVAALGHTTEAGDAPSPRSGRVAELEDEVRRLRQQVDWLTAE
                     FEAFRKQFQ"
     gene            23320..>24085
                     /gene="lgt"
                     /locus_tag="PLH85_09660"
     CDS             23320..>24085
                     /gene="lgt"
                     /locus_tag="PLH85_09660"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB64871.1"
                     /translation="MLVHPQFNPVALSIGPLDVRWYGLMYLTAFILFVALGRAHARRR
                     PELGWDAQQIDDLLFYGVLGVILGGRLGYILFYKPAYYFSHPVEMFAVWQGGMAFHGG
                     FLGVLIAVWLFARSHDRPWLAVTDFIAPLVPLGLAAGRLGNFINGELVGRVTDVPWAM
                     IFPQVDMQPRHPSQLYQFTLEGVCLFVLLWVFSAKPRPRGLVSAVFLIGYGGFRFLAE
                     FGREPDDFLGLLALNLSMGQWLSLPMIVIGIVMAVIA"
BASE COUNT         3740 a         9159 c         7454 g         3632 t
ORIGIN      
        1 cggaaccgtt gtcaccgacg atgggcttgg gcatgaaggt ggcggtcttg ccgtactggt
       61 gcgccacgtt ctggacgatg tacttcagga tctgggtctg gtcggcacga cgcaccaggg
      121 tgttgaactt ggtgccgatt tcgttctggc cggcgttcgc cacttcgtgg tggtgcactt
      181 cgacggggac gccgcaggct tccagagcca gacacatggc ggcgcggatg tcgttgaagg
      241 agtcaaccgg aggcacgggg aagtagccgc ccttgaccgc gggacggtag ccggtgttgc
      301 cgccttcgta cttgatgtcg gacgaccagg caccttcttc ggagaagatc ttgtggtagg
      361 cgccggacat gtccaccttc cactcgacgg agtcgaagat gaagaattcg ggttccggac
      421 cgaagaacgc ggtgtcacca atgccggaag ccttcagata ggcttcagcg cggttggcga
      481 tggagcgcgg gtcgcggtca tagcccttgc cgtcggcggg atcgatcagg tcgcaggtca
      541 ggaccagggt ggtttcgtcg aagaacgggt cgatgtaggc ggtgttgggg tccggcatca
      601 gcagcatgtc ggaggcctgg atccccttcc agccggcgat ggaggaaccg tcaaaggcat
      661 ggccgctttc gaacttgtct tcgtcgaagg cgctgagggg cacgccgacg tgctgttctt
      721 tgcccttggt gtcggtgaaa cggaagtcga cgaacttgac ttcgttttcc tggaccatct
      781 tcatgacgtc ttgcggggtc atataactca ctcctaacgg gttgaagctg ctgcgtagtg
      841 gataaaggaa caaaaccagg aattcgggta tcgctcccgg tagcaccaag cgtgccaccg
      901 ccgggaaggg atcacgctat tgtgccataa gggttaactg ctgccagccg gaaattgacc
      961 cgcaccactc aagggcccct tcaacacaaa cgcaccacta tggtgcaatc tgacacagca
     1021 gcgcgatgcg cccccagtga ggccggcggg caacgccgtc cgccacctgc tgccgcaggg
     1081 cgtcgatgcg gcgcgcatcg caacaccaga catggggcag aaagacgtcc gcctccacct
     1141 gcttgccgac atagtgcagg atgatccgct ccgtctgcgg gaggtccggc ccgaggatct
     1201 cggcgatctc cgccaggagc acgtcccggg acggcagatg gcggccggcg gcgagttcct
     1261 cgtcctcttc cgtatccaca tggaccagca cgtcgagcac gtcctccacc cggctgcgca
     1321 cgcggtgacg ggccagctcg gcgatgcgat ggccttccga cacactgata cgcgcatcca
     1381 cccgcacgtg ggcgtccacc aggatgcgat gggccatgcg ccgcgtgcgc aggtcgtgca
     1441 cccccagcac gccgggcgtt tcttccaccg cacggcggat ggacgccacc tcttccggcg
     1501 ccacgccggt gtcgatcagc tcgcgcaagg cgtcgaagga cagttgcagc cccatgcgga
     1561 tgatcatgaa accgaccagt gccgcggcga ggggctccag aaagcggtag cccagcaggc
     1621 tgcccccgat gccgaccgcc accaccagcg acgacgctgc gtcggcgcgg gcgtgccagg
     1681 cattggcgac cagcatcggc gagcgaaggc gctcggccac cgccagcatg tagcggaaca
     1741 ggccctcctt gccagccaga gtcagcagcg ccatccacag ggccgcggga tgcagcgcgg
     1801 gcaaggcttc ggtgctctgc agacggctgg cggcggagag caggaagccg gctcccacgc
     1861 cggccagcac cagcccgagg atcatcgacg ccgccgtctc gtagcggccg tggccgtagg
     1921 gatggcgctc gtccgccggc gtggagccgc gccggctcgc caccatcacc atgaaatcgc
     1981 cgagcaggtc cgacagggtg tgcataccgt ccgccaccag actctgggca tgggcgaaca
     2041 gccccacggc gatctgcagc accgtcagga caacattgac ggcgatgctc acccaggtgg
     2101 cctgctgggc ctgacgggcg cggtcggcat cgaggggcgc ctcggtggcg gccgagagag
     2161 gggaatcgtg ggccatgact tgcagcggaa gggcgcgggg gtatactcgc aggcccgcca
     2221 ttctagccgg atgtccgcca tgggaaaatt aagcgagacc ctcgcactgg cgcagcaacg
     2281 cagccgcgac gccggcctgc cctacgccgg cctgctcacc ccccgcgaag cctgggacct
     2341 gctccaggcc gcccctggcg ccaggctggt ggatgtacgc acccgcgccg aatgggactg
     2401 ggtcgggcgc gtgcccggcg cggtcgagct tgaatggatg ctctaccccg gcaacgagcc
     2461 caacccccac ttcctcgccc agttgcagcg ccaggtcgat ccggaagccc tggtgatgtt
     2521 cctgtgccgc agcggggtcc gttccaacaa cgcggccacc ctcgcggccg gccacggtta
     2581 caccaacgcc tacaacatcc tggagggctt cgagggcgac aaggacgccc acggccatcg
     2641 cggcaagctg ggaggctggc gcttcgccgg tctgccgtgg atccagggct gacaccgact
     2701 cacaggattg acccgacgat gcagaccgcc tccatcagct tcgaccgctt caacgccctg
     2761 gccgacgccg acgcccacgc gcgcatccgc gccgcccgtg cccgcctcgg cgacaaggcc
     2821 gtgctgctgt gccaccacta ccagcgcgcc gacgtctacc agtacgccga catcaccggc
     2881 gactccctca agctttcccg cctcgcttcg gacaccgacg ccgagtacat cgtcttctgc
     2941 ggcgtgcact tcatggccga agtggcggac atcatgtcca ggccgcagca gatcgccatc
     3001 ctgcccgatc tggcggccgg ctgctccatg gccgacatgg ccaatctggc caaggtagaa
     3061 cgctgctggc gcgaactgac ggaagtgctc gacacgccgg atgccctgat caccccggtc
     3121 acctacatca actccgccgc ggatctcaag gctttctgcg gcgagcacgg tggcatcgtg
     3181 tgcacctcca ccaacgcccc ggtgatcctc gactgggcct tcgcccagcg ggagaaggtg
     3241 ctgttcttcc cggaccagca cctgggccgc tggaccggct tcaacaaagg cattccgctg
     3301 gacgaaatgg tcgtgtggga tcccgacctg gaatacggcg gcctcaccgc cgaacagatc
     3361 cgcaaggcca ggatcctgct gtggaagggc cactgctcgg tgcaccagat gttccagccc
     3421 gcccacatcc tcaagttccg caatcagcat ccggacggca aggtcatctc ccacccggag
     3481 agcgccctgg aggtgtgccg ccagtccgac ttcgtcggct ccaccgagta catcatcaag
     3541 gtcgtcaagg aatctgcccc cggcacccgc tggctggtcg gcaccgagct caacctggtc
     3601 aaccgcctcg ccgaggaagt gaagcacgag ggcaagatcg tccagttcat ggcgcccacc
     3661 gtgtgcatgt gctcgaccat gcagcgcatc gacccgcagc acctggcgtg gaccctggag
     3721 aacctcgccg agggcaacgt ggtcaaccgc atcaccgtgc ccgcccacga ggcggccctg
     3781 gccaaggtgg cgctggaacg gatgctcgcc gtgtcctgat cctgcgccag cacctcaaga
     3841 cggcgcccgc gggcgccgtt tttcctggcg gccatccctg cggacgaaga aggaacggac
     3901 atgagcaccc tgcgcatctg cacctacaac attcacaagg gcttctccca gttcaaccgc
     3961 cgcctcgcca tccacgacgt gcgcgagcgc ctgcaccagc tcggcgcgga cgtggtcttc
     4021 ctgcaggagg tccagggcca gcacctccac cacgcccgcc gccacaccga ctggcccgaa
     4081 caaccccagc acgaattcct cgccggcgac ctgtggcgcg acaccgcgta cggcggcaat
     4141 gccctctacg accacggcca ccacggtaac gcgatcctca gccgccatcc catcctgtcc
     4201 cgcgccaacc aggacgtctc cgaccaccgt ttcgaacggc gcggtctgct ccattgcgag
     4261 atccggctct ccggcgtggc gcgcccggtg cattgcgtct gtgtgcatct ggggctcacc
     4321 gccggcacgc gcaggcgcca gatggcggcg ctggtggcgc gcctcaacgt cctcgccccg
     4381 cccaccgagc ccctcatcat cgctggcgac ttcaacgact ggcgcaacca cgccgacgac
     4441 cacctgatcc gccagctcgg cgtcgtcgag gtgttcaccg ccctgcgcgg ccgaccggcg
     4501 cgcagcttcc cgtgccacct gccgatgctg cgcctggacc gcatctacgt gcggggcttc
     4561 gccatcgacc aggcggaggt gcatttcgga gcaccgtgga accgcctgtc cgaccacgcc
     4621 cccctcaccg cccagctgcg cgcccactga gctgccgcca tggccatcca ctggctgcct
     4681 ggcaacgccc tcaccctgct ggagaacggc gcggaattct tccccgccct gcaggccgcc
     4741 atcgacactg cctgccacga ggtctatctc gaaacctaca tctttgcgga tgacgttacc
     4801 ggtcgcgcca tcgccgccgc cctggcccgc gcggcggcgc ggggcgtcac cgtgcgcctg
     4861 ctggtggacg gcttcggcgg acgcgaattc gtcgagcggc tgcgggacga cctgatcgcc
     4921 cgcggcgtcc atgtcctcat ctaccgccgc gacatcagtc ccttcgccat ccgccgtagg
     4981 cgcctgcgcc gcctccaccg caagctggcg gtcatcgatg gccgcgtcgc cttcgtcggt
     5041 ggcatcaaca tcctcagcga cctgtcggag cccaacatgg cggccccgcg ccacgactac
     5101 gcggtgcgcg tcgaaggccc cctcctcgcc cccatccacg ccaccgtgca gcacctgtgg
     5161 gaactggtcg cgtgggcctc cttccgccac cgtcaccgcc ttccggccgg cccggcgccc
     5221 tgctgcggca cctgtggcga cgtggaggcc gccttcctga tccgcgacaa cctgcgccac
     5281 cgccgcgaca tcgaggacgc ctatctgctg ggcatcgccg cggcacgccg cgagatcgtc
     5341 ctcgccaacg cctacttcct gccggggcgg cgcttccgcc aggccctgct ggacgccgcc
     5401 cgccgcggcg tgcgcatcac cctgctgatc cagggcctcg ccgaccaccc gctccagcag
     5461 cgcgccaccc gcgccctcta cggccagctc ctggccggcg ggctgcgcat cgtcgagtac
     5521 caccgcagct acctgcacgc caaggttgcc gtggtggacg agcgctgggc cacggtgggc
     5581 tcatcgaaca tcgacccctt cagcctgctc ctcgcccgtg aggccaacgt ggtgatccgc
     5641 gacgccgcct tcgccaccca cctgcgcgac catctccagg acgccatcgc ccacggcggc
     5701 cgcgagatct ctgccgacgc gtggcagcgg ggcccacgcc tgcaacgcct gctcaactgg
     5761 gccgcctacg gcctcgtgcg actgatgatc ggcctcgccg gctacggcgg ccggcactga
     5821 gaccgtcgcc cggccggaac atgggacaat ggacgcctcc tccgtcccgc cgccagtctc
     5881 catgaactac cccgcccccg ccttcgaagc caagatctgc cacccgcagg acctggccgc
     5941 ccgcgtcgcc gcgctgcccc gtccgctggt gttcaccaac ggctgcttcg acatcctgca
     6001 ccggggccac gtcacctatc tggcccaggc ccgcgccctg ggcgccgcga tggtggtggc
     6061 cctcaacagc gacgcctcgg tgcgccgcca gggcaaaggc gacgatcgcc ccatcaacgt
     6121 cctcgaagac cgcctcgcgg tgatggccgc cctggactgc gtggacctgg tcacctggtt
     6181 cgacgaggac acgcccatcg cccgcatcct cgactgccag cccgacgtgc tggtgaaggg
     6241 cggcgactgg cccgtcgaac gcatcgtcgg cgcccccgaa accttcgccc gcggcggcca
     6301 cgtgcactcc atcccctttg aacacgagcg ctccaccacc gccctgctga cgaagatccg
     6361 caggctgtga catcggggcg cggggcactt tgtttattcc aatttgaact attgattcac
     6421 gtcaaccagc actagaatta attagccccc ccctcacccc gcccgcaagg agccgccaag
     6481 atgcgcatcg ccagcaatgt ggccgaactg atcggcaaga cccccctcgt ccgcctcaat
     6541 gcactcgccc cggccaacgg cgccacgctg gccctcaagc tggagttctt caacccggcc
     6601 cacagcgtca aggaccgcat cgcagcggcc atgctcgacg ccgccgaagc cgccggcaag
     6661 atcaagccgg acaccatcgt cctcgaaccc acctcgggca acaccggcat cggcctcgcc
     6721 atggtctgcg ccgcccgcgg ctacaagtgc gccttcgtga tgcccgagac gatgagccgg
     6781 gagcgcaagc tcatcctcaa ggcctacggc gccgagctga tcctcacccc cggcgccgaa
     6841 ggcatgcccg gcgccgtcaa gcgcgccacc gagctggccg ccgccgaccc gcgctacttc
     6901 atcccgcagc agttcgaaaa ccccgccaac ccggccatcc accgagccac cacggccgag
     6961 gagatctggg ccgacacgga cggccaggtg gacatcttcg tgtcgggcat cggcaccggc
     7021 ggcaccatca ccggcgtcgg cgaagtgctc aaggaacgcc gccccggcgt gaaggtcatc
     7081 gccgtcgaac cggccgccag cccggtgctg tccggcggcg cccgcgggcc gcacccgatc
     7141 cagggcatcg gcgccggctt cgtgcccgcc atcctcaaca ccgcggtcta cgacgagatc
     7201 gtccaggtgc ccaatgatgc cgccctcgac actgcgcgcc gcctggcgag ccaggaagga
     7261 ctgctggtag gcatctcctc cggcgcggcg gtgtgggccg cccttcaggt ggccaaccgg
     7321 cccgagaacg ccggcaagct ggtcgtcgtg gtgattccgt ccttcggcga acgctacctg
     7381 tccaccgtgc tctaccagca cctggaagtc tgaatcaccc cctccgcagg acgcgccccc
     7441 ttgtggtcgg gcgcgtcccg ttgcaagctc acggccagac accattccag gcctccgccg
     7501 tgaccctccc cttcgacttc gcccacggcg ccgaacgcgc cggctccgac agcatcaagt
     7561 ggcgccgcta cgccggccgc gacgtcattc ccatgtgggt ggccgacatg gatttcgccg
     7621 cgccgccgcc ggtcctcgac gccctgcgcg cgcgcctcga ccacggcgtg ttcggctacg
     7681 ccgatccctg gcctggcgta ctggaagccg tcatcgacgg catcgcccgc gaccacggct
     7741 ggcgcatcca gcccgaatgg ctggtgtggc tgcccggcgt cgtcaccggc ttcaacgtgg
     7801 ccgtgcgcac cgtcgggcgg cccggcgacg gcgtcttcac cgccacgccg gtgtatccgc
     7861 ccttcctcca cgcgccgggc aatttcgagc agcgcctcgt caccacgcca ctcctgcgca
     7921 caggcacgcg ctgggaatgg gactgggacc gcagcgcggc cgccctcgcc gacggcgtgc
     7981 gcctcttcat gctgtgccat ccccacaacc cggtcggccg ggtcttcgac cgggacgagc
     8041 tcggccgcat cgccgaactg gccgaacgcc acgacctggt ggtgtgttcc gacgagatcc
     8101 actgcggcct ggtcctcaat ccgaccaagg ctcacgtgcc ccttgccgcc ctcgacgagc
     8161 gcatcggccg acgcaccatc acgctgatgg cgccatccaa gacctggaac attccggccc
     8221 tctactgcgc cttcgccatc attcccgacg gcaccctgcg ggcccgctac aagcacgcca
     8281 tggccggcat cgtgccccac gtgaatgtgc tgggactggt cgccaccgaa gccgcctacc
     8341 gggacggcgg cccctggcgc gaagccctcc tcgccaccct gcggagcaac gccgcccgcg
     8401 tcacggacgc catcgacctg ctccccggcc tcgccacgac gcccgtcgaa gccacctacc
     8461 tggcgtggat cgactgccgc gacttcctcg ccgcccgcgg cctcacgggc agccccgcgg
     8521 ccttcttcga gcaggccggc gtgggcctgt ccgacggacg cgacttcggc ctgccgggct
     8581 tcgtgcgcct caacttcggc tgcccgccgg cgcgcctgga ggccgccctg gagcgcatgc
     8641 gccgcgccgt cacggcctga tgggcgcgtc accggccagc ttcggcctgc gcacggcgcc
     8701 gcgggacgtc cgcctctacg tcgtcaccac ggtgtgcagc gccctgatcc acttcggcca
     8761 gggcgtcgcg gtgggctggt cggtgtatga gcagacgggc gcgcccctgg ccctcggctt
     8821 cgtcggcctc gcccagttcc tgcccatcgc cgtgctcttc ctgccggccg ggcatctggc
     8881 ggaccgggtc gaacggcgcc gcatggtcct cgtcagcctg ctcctcacgt tcgcggccac
     8941 cctgctgctg gcgtacgccg catggcgcgc cctgccggtg ggctggacct acgcggcgct
     9001 ggtcctctcc ggctgcggcc agatcctcta ccgaccggca cgggacgccc tgctgccggc
     9061 cctgctgccg ccgcagcatc tggcccgcgg catcgccctg gcctcggccc tgtaccaggt
     9121 cgcctccctc gccgcaccgg cggcggtggg tttcggcctc gcccaggtcc attccgccgt
     9181 gcccatccac ctgtgcaacg ccctgctggc ggcgctggcc ctgctcgcta ccatcgccat
     9241 ccgccagcgc tcccacggcc agcccgccgc caccggcagc cgcgtcgcgg aggtgctggc
     9301 gggcttccgc cacgtgtggc gcacccgcat cctgctggcc gtgatgtcgg tggatctggt
     9361 cgccgtgctc ttcggtggcg ccgtcgccct gctgccggtc tacgcccgcg acatcctcca
     9421 cgtggggccg gccgggctgg gcctcctcag caccgcgcca gcgctcggag ccgcctgcgc
     9481 cgggctggtc atgacccgca gcggcgtggc cctggacggg cgcgtgctgc tcgccgcggt
     9541 gggaggcttc ggcgtggcca ccgtggtgtt cggactctct acggcctttc ccctgtccct
     9601 tgcagccctg gcggtggcgg gtggttgcga ctgcgtcagc gtggtgctgc gccagaccgt
     9661 cctccagcag gccacgccag atgcgctgcg cgggcgggtt ggcgcagtga accgggtctt
     9721 cgtgagctcg tcgaacgagc tcggcgccct cgaatcgggc ctcgcggcgg cctggttcgg
     9781 accgctcctg gcggtcgccg gcggcggtgc gatcaccgtg ctggtcgccc tcggcgcacc
     9841 cgcgctcttc cccgtgctgc gcgccctgca gcacgaccgg cctctgccgc gcgaccccgc
     9901 cgtttcctga tccagcgcaa gattccgtca tggcggcggc gcatactggg taacgtcgac
     9961 gccccaggag acgcgcgtga cccctcttcc gtccgccccg gccgatgccc tcgacggcat
    10021 ccttcagcgc catcgtcacg acgccacccg cctgctccag atcctcatcg acgcccagga
    10081 tctcgacggc tggctgccac ctcccatgct tacccgcatc gcagccgccc tggatctgcc
    10141 gcgcgcccgg gtggaagggg tcgccggctt ctacagcttc ctctacaccc agcccgtcgg
    10201 ccgctaccgg gtgctgttct ccgacaacat taccgaccgc atgctgggca acgccgggct
    10261 gatggacgcc ctctgcgcca agctgtgggt ggagccgggc aagcgctcgg aagacggcct
    10321 cgtgagcatc gccaccacct cctgcaccgg cctgtgcgac caggggccgg ccttgctggt
    10381 gaatggccgc gccatcggcc agctcgacca ccgccgcatc aacgccatcg ccgaactgat
    10441 ccgccagcag acgccccttg aacgctggcc ggcggagttc ttccgggtta cccacaacat
    10501 ccgccgccgc gatgccctgc tcaatctgga catggcgccc ggcgcggccc tcagcgcggt
    10561 cctcgccctc ggccgcgaag gctggctcac cgagatggag gcctcccgcc tgcgcggccg
    10621 cggcggcgcc ggcttcacca cttcggtcaa gtggttgagc gcccgcaacg cgccgggcgc
    10681 cgagaaggtc atcgtctgca acgccgacga aggcgagccc ggtaccttca aggaccgggt
    10741 gctgctggcc ggcttcgccg acctggtgtt cgaaggcatg acccttgcag cctgggccgt
    10801 gggtgcacgc cagggcctgc tctacctgcg cggcgaatac cgctggctgc gcccccatct
    10861 ggacgcggtg ctggcccggc gtcgctccgc cggcctcctg ggcccggcca tcctcggtga
    10921 acggggcttc gatttcgaca tcgacatcca cctgggcgcc ggcgcctacg tgtgcggcga
    10981 ggagacggcc ctcatcgaat ccctcgaagg caagcgcggc acaccccgca tccgcccgcc
    11041 cttcccggtc acccacggct acctgggccg tcccacggtg gtgaacaacg tggagaccct
    11101 ggccaaaacc tgcctcatcg ccctgcaggg cggcgcggcc ttcgcggcca ccggcaccgc
    11161 ccagtcggcc ggcaccaagc tgctgtcggt gtcgggcgac tgcgccgcgc cgggcatcta
    11221 cgaatacccc ttcggcgtca ccctggcccg cgtgctggag gactgcggcg ccagcgacac
    11281 ccaggcggtg caggtgggcg gcgcggcagg cctgtgcctg gcgggctacg agttccagcg
    11341 ccgcatcgcc tacgaggacg tccccaccgc gggcgccttc atggtcttcg acgcgacccg
    11401 cgatctcttt gaggtggccc gcgccttcgt tcatttcttc gcccacgaga gctgcggctt
    11461 ctgcaccccc tgccgggtcg gcacggcgct cctcaagggc ttcatggaca agctcgccga
    11521 cggccacggc gcccaggctg acctggccga catcgaatgg ctcgaccgcc tgctcaagaa
    11581 cgcgagccac tgcggcctcg gcagcgccgc gccgaacccg gtcatcgaca ccctgcagaa
    11641 gttccgcccg gcctatgagc ggcgcctgct gtccctggac ttcgagcccg ccttcgacct
    11701 ggacggcgcc ctcgccgaag cgcgccgcct caccgcccgc aacgaccccg gcgcccacct
    11761 ggccacccac gaggagacac gggaatgagc aagaccttcc tgctggacgg ccgcccggtg
    11821 cccttcgagg acggccagag catcctccag gccgcccgcg cggcgggaca gttcatcccc
    11881 agcctgtgct ggcacaagga cttcccggcc cacggcagct gcaagctgtg catcgtcaag
    11941 gccgccggac gtcacgtctc ggcctgcgcc acgccagccc gggagggctg ggaagtcgag
    12001 agcaacacgc cggagatgaa caacgagcgc cgcgccctgc tgcagatgct gttcgtggag
    12061 ggcaaccact tctgcccgtc ctgcgagaag agcggtcact gccagcttca ggccctggcc
    12121 tacgaatacg agatgctcac gccgcgcttc cgtcacttct acccggagcg gccggtggat
    12181 gcatcccacc cggacatgct gctggacttc aaccgctgca tcttctgcga actgtgcgtg
    12241 cgcgccagcc gcgacgtgga cggcaagtcg gtgtttgccc tcacgggccg aggaattgcc
    12301 aagcacctca ccgtcaatgc cgaatcgggc cgcctcgccg acaccgactt cgcagcgacc
    12361 gacctggctg cgcggatctg cccggtgggc gtgatcctgc ccaagggccg cggcttcgcc
    12421 gtgcccatcg gcgcgcggcc ctacgacacc ggcacgatcc gcgacacggc gcaggaggat
    12481 tagcatgaac cggctccgtc cctcactccg tccgccacct gcgctccggt cgccgcgcct
    12541 cggcgcagcc gcacgggcgc caggccccga tgcttcatcc cgccccacgc gcagccgcag
    12601 cgcgctcacg tctctcctgc ccggaggccc gacatgaccg ccccccgcaa gatccgcgtc
    12661 gccacggcgt ccctcgccgg ctgcttcggc tgccacatgg cctttctgga catcgacgag
    12721 cacctgctgg agctggccgg gcaggtggag ttcgaccgct cgcccctcac cgacatcaag
    12781 gacgtgggcc cctgcgacgt gggcctcatc gaaggcggcg tgtgcaatgc ggaaaacgta
    12841 cacgtgctgc gcgccttccg gcgccagtgc aagaccctgg tcgcgatggg cgcctgcgcc
    12901 atcaacggcg gcctgccggc ccagcgcaac cacctgtcgg tggcggactg cctgcgcgag
    12961 gtgtaccagt tccggcccgg cctcgccgat gggcgcatcc cggacgaccc ggaactgccc
    13021 ctgcccctcg acaaggtacg ccccctccac gaggtggtgc gggtggatta cttcctgccc
    13081 ggctgcccgc cctccggcgc ggcggtgtgg aagtttctct ccgatctgct ggcgggccgc
    13141 accccgcgtc tcaccggcgc cctgctccac tatgactgag gtccggccat gagcctcgaa
    13201 ctggaaaccg ccgcagaccg ggcacacctg cgccgcgtcg ccatcgaccc ggtatcccgc
    13261 gtggaaggcc acggcaaggt caccctcctg ctggacgagg gcggccacgt gcaggaggcc
    13321 cgcctgcaca tcgtggaatt ccgcggcttc gaaaaattca tccagggccg gcccttctgg
    13381 gaagtgccgg tgatggtgca gcgcctgtgc ggcatctgcc cggtgagcca tcatctggcc
    13441 gccgccaagg ccctcgacac cgtggtgggc gcgacgatca cgcccaccgc cgagaagatc
    13501 cgccgcctga tgcactacgg ccagatcctc cagtcccacg ccctgcactt cttccatctg
    13561 gcctcgccgg acctgctgtt cggcttcgat gcgccggccc agcagcgcaa catcatcgcc
    13621 gtcgccgccg cccatccgga catcgccacc catggcatcc tgctgcgcaa gttcggtcag
    13681 gaagtgatcc gcatcactgc aggcaaacgc atccacggca ccggcgcgat ccccggcggt
    13741 gtcaataagt ccctcagccc cgccgagcgg gacgaactgc tgcgcgacgt ggacaccatg
    13801 atcgactgga gccgcgacgc cgtggccctc gcccgccgcc tgcacgagag cgatgcggat
    13861 ctctacgacc atttcggcga agtgcccggg cagttcctca gcctggtgcg ccccgatggc
    13921 gccctcgacc tgtacgacgg cgtcctgcgt ctgcgcgggg ccgacggccg tgtggtgcgg
    13981 gacggcatcc cctgcaccga ttaccttggc cacatcaccg aggcggtcag gccctggagc
    14041 tacatgaagt tcccctacat caccacgcag gggccggaca ccggctggta caaggtcggc
    14101 cccctggcgc gggtgcagaa ctgcgacctc atccccacgc cccgcgccga ggcggcgcgc
    14161 caggccttcc tcgcccacgg cggcggcgcc ccgctgcatg gtccgctggc tttccactgg
    14221 gcgcgcatga tcgagatgct ccacgccgcc gaggtgatcc gcgagctcct cgacgatccg
    14281 gacctgctct ctgacgaccg gatgtctggc ggcccgcgtc ggcgcgaggg catcggcatc
    14341 atcgaagccc cccgcggcac gctgatccac cattaccagg tggacgacga cgatctggtc
    14401 agcgactgca acctgatcgt gtccaccacc cacaacaacc aggcgatgaa caccgcggtg
    14461 cgcgccgtcg cccgccgcta cctggacgga cagacactga ccgagggcct gctcaaccac
    14521 atcgaggtgg cgatccgcgc cttcgacccc tgtctgtcct gcgccaccca cgcactgggc
    14581 aagatgcccc tggcgctgga actgcgggac atggccggcg tcctggtgga tcgcctgcaa
    14641 cgggacacct gaacggcatg ggcgtggtga tcctcgccgc cggcaatccc tcgcgcggcg
    14701 acgatgccct cgggcctctg ctgctggccg agctggccgc tctggcgccg gacggggtca
    14761 cgctggtgga ggatttccag ttccagatcg aacacgcgct ggatctggtc gggcaggaca
    14821 ttgccctctt catcgatgcc gcggcaagcg gcgcagcccc cttctgcttt gcagagatcc
    14881 ggccggcccg cagccgcgca ccgagcagcc acgccctgag ccccgacgcg gtgttgcagg
    14941 tgtatgcgga gaccctcggc gtgccgccac cggctttcgt actggccgtg cggggcgcat
    15001 ccttcgcact gggggcgccc ctgtcgtctg tggcccaggc tcatctggag gcggcgcgac
    15061 cgcttctggc ggcctgtctg gcccacccgg acgtggacgc gtggcggcgc ctcgtgcatc
    15121 cgcgcccgtc cgcagcggac tgaatcaggc cgcgacgcga cgctgggagt attcgttgag
    15181 cagcagggaa atggcgtggg cggcgccttc cggcagattc agggttttcc gcagctgcgc
    15241 cacaaacacc ggacaccccg cgccatggtt cggaccgcgg accagcagga gctggttgca
    15301 ggggacgtcc tgccaggcct cggacgacac gtcgatatcc accagcaggg acaaggccgt
    15361 cccgtgctgc cacaccgcca gatcgtcggc gaagcgtgac agggccatgt cccgctccga
    15421 ctccccgcaa cgtccgaaac cggaactcca cacgagttcc tcgtgcaagc gcgtgccact
    15481 tccgtcgagg acgccgatcg ccacccgctc cgcgtccagg gcacggagca gctgacgcag
    15541 ggcccgcagg ctcgcaaagc gcgcatcgcc caggtggcgc acgaaggcgg cgccggtcag
    15601 ggcggcgtcg gaaaccgggt ccgtcatgta gcccgacggt gcacccgccg cgtgccactg
    15661 ggtgccgtcc agggcccctg cttcacgtct gatctcaagc atcgcgtctg ctcctcaatg
    15721 cggctccgcc gggcggagcc tgattccgga aggagggtcc ggtcgcgggc tttttttccc
    15781 gtttcgcggc gccatccgat gacgggaaag tgtacggatt catccgtccg gcattcgtgc
    15841 acaattccgc agacattctg ccacaaatca caaaaacatc gaatttgtca caaacattca
    15901 aatcatgccg tccgcggcgg aggctgccgg ccgccagact ccgtacctcg catcgggcgg
    15961 catcacgttg tgacggcacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 gccggccgcc agactccgta cctcgcatcg ggcggcatca cgttgtgacg gcaccatgaa
    16141 aaagccctcc ggatagcgga gggctttcag gggcaaccgc gccgacgacg gcgcagggtg
    16201 gtcaggccgt cttgcgattg cgctgcatgg ccatgatctt ggcctgcaca tagcccttca
    16261 gctcgcggaa ggggacaggc ttgccataca aggtcacgtc tgccggcagg ccgccgttct
    16321 cggcgatttc ctcgtcggtc aggccgctca ccacgacgat gtccagatcg gccagctgcg
    16381 gatcggcccg caggcggcgg atcatctcga agccgtcgat gcccggcatg cgcaggtcgg
    16441 tgatgagcag atccggcggc atgcggccga tttccagcag gccgtcgatg ccgctcgcca
    16501 ccatgcgcac ctggacggcc agcccccagg acgagaacgt gtgctcgtag agcttctgca
    16561 agatgccatc gtcctcggcg accagcacct gcaggacacc gggcggccgg gacacggtct
    16621 gaccgccacc ggccgccgcg cgggcccgga agtcctcgat ggaactgacc cggatccggc
    16681 gatgcccgcc agcggtcttc cacccttcca gcaggccgtt ttccaccatc tgctggacgg
    16741 tacccagcga aagacccagc cgctgggcgg cttcggtcgt gctgcagtat tccttttccg
    16801 gaacttcagt gttggcagac atggtcaggg cagcaggcga tcaacgtgaa caggcatgat
    16861 cttaacagta acgaaacgta tggaataccc atacacctac aactaaagga ataggcagta
    16921 gagtatcaga atgggatatc gtcatcaaaa tcattgaatg ccgcgccgcc cgcaggcttg
    16981 cggggcggtg cggacggggc cggagccgcg gccggagcgc cgccgaaggc cgtctcccgc
    17041 ggtgcggcgc cgccgtatcc accgccatat cccccctcgc tgcgagccgg ggcgtcgccg
    17101 ccccccatgc cctgccggct gcccagcatc ttcatctcgt cggcccggat ctcggtggtg
    17161 taacggtcct ggccatcctt gtcctgccac ttgcgggtac gcagggagcc ctcgatgtac
    17221 acctggctgc ccttgcgcag atactggccg gcgatttcgg cgagcttgcc gaagaaggcc
    17281 acccggtgcc actcggtcgc ttcgcgcttc tcgccgctgg ccttgtcgcg ccacgtgtcg
    17341 gtggtggcga cggtgatgtt gcagatcgca tcgccgctgg gggcgtagcg cgtttccgga
    17401 tccttgccca gattgccgac gaggatcacc ttgttgaccg atgccatagt tctctcctga
    17461 atgtaagtcc gatggagccg cgccgatggg tggcgggcag gggggcgggg cggatcaggc
    17521 cgggacgacg cgccgggccg gcggcgtcat gccggcggcg agcgccagcc acaccagagc
    17581 caggccgccg cccagcaggg acaccgcacc gggcccctcg tgctgcgcca gccagccacc
    17641 gagcgctccg ccaaggaaca gcccgaggga ctgcaacgtg ttgtacaccc cgagcgccgt
    17701 ccccttggcg tgggccgggg cgatcttgga aatccacgac ggctgggtgg cctcgaggac
    17761 attgaaggcg acgaagaaca gggtcagcag gatggccagc ggccacaagc ccgccgaacc
    17821 ttccacataa agcccgatct gcaccaccag cagcaaagcg atggcggtgt tgaagaccgg
    17881 cttcatggcc cctttgcgct ccgccatgat gatggccggt accatgaccg caaaggacag
    17941 cagcacggcc ggcagataga tcttccagtg ttccgccacg ggcaggccgg cgcgcaccag
    18001 tcccgccggc accagcaccc acatggccgt ctgcatgagg tgcagggcaa acacaccgaa
    18061 gttgaggcgc atgagctggc cattgccgag cacctcggca aaacgcccgg tggcccgcgg
    18121 caccggcggc gcggcgggca ccacgaacag caccgtgagg atcgcgcccc ccgccagcac
    18181 ggcagtcagc cagaacagcc cgctcatgcc gatgatcccg taaagcaccg gcgccgccac
    18241 catggagagg gcaaacacca ggccgatgga cgagccgatc atggccatca ccttggtgcg
    18301 gtgctggtcg cgggtgaggt cggcggccag cgcggtgacc gccgcagaaa tggcgccggc
    18361 gccctgcagc acgcggccca tggcaatcgt cgtcacatcc tgggcaagcg ccgccaccac
    18421 gctgccggcg acgaagagca gcaggccgaa gacgatcacc ggcttgcgtc cgaagcggtc
    18481 cgacgcggtc ccgaagggaa tctgcaggaa agcctgggtc aggccatagg cgccgatggc
    18541 aaagcccacc aggcccacgt tgtcgccgcc gggcagaccg tgggcatgca ccgcgaacac
    18601 cggcaggacc aggaacaggc ccagcatgcg cagcgcaaag atcgccgcca gcgaaacgcc
    18661 tgcacggcgc tcggccggac tcatcttgtc gtcgttctgg gaagccatga atcgtgctgc
    18721 gtcgggaggg ggaagaggcg tatattaaca ggttgccccc accccgaatg ccccatggac
    18781 gccatccgaa tccgcggtgc ccgcacccac aacctcaaga atgtcgccct cgacatcccc
    18841 cgcgaccggc tcaccgtgat caccggcctg tcgggctcgg gcaagagctc cctggctttc
    18901 gacaccctct acgccgaggg gcagcggcgc tacgtggaat ccctctccgc ctacgcccgg
    18961 cagttcctgc agctgatgga aaagcccgac gtggacctca tcgagggcct ctccccggcc
    19021 atctccatcg agcagaaggc caccagccac aacccccgct ccaccgtcgg caccgtcacc
    19081 gagatccacg actacctgcg cctgctctac gcccgcgccg gtacgcccca ctgtccggac
    19141 caccccgagc acgccctgga ggcccagagc gtgtcccaga tggtggacgc cgtgctggcc
    19201 ctgccggaag acacccggct gatgatcgtc gcgccggtca tctccggccg caagggcgaa
    19261 caggccgacc tcttcgccga gctgcgcgcc cagggcttcg tgcgcgtccg cgtggacggc
    19321 gccgttcacg agctggacag cctgcccgcc ctcgacaaga accgccgcca cagcatcgag
    19381 gtggtggtgg accgcttcaa ggtgcgccag gacctgcgcg ggcgcatcgc cgaatccttc
    19441 gaaacggccc tcacccacgc cgagggccgc gccatcgccg tggagatgga cagcggcacc
    19501 gagcacctct tctcggcgcg cttcgcctgt ccggtgtgca gcttcgccct cgccgagctc
    19561 gagccacgcc tgttctcctt caacaacccg gccggcgcct gtcccaggtg tgacggcctc
    19621 ggcaccgtgg agttcttcga cccggcgcgg gtcgtcgccc acccggagct gtccctcgcc
    19681 gccggcgcca tccgcggctg ggaccgccgc aaccagttct atttccagct cctcgccagc
    19741 ctcgccagcc actacgaatt cgacatcgac acgcctttcg agtccctgcc ggacgccctc
    19801 cgccaggtgg tgctgcacgg ctcgggacgc gacaagattg ccttccgcta cctgggcgac
    19861 ggcggcaagg cggtgctcaa ggaacacgcc ttcgaaggca tcatccccaa cctggaacgc
    19921 cgctaccggg agaccgactc cctcgccgtg cgcgaggagc tcgccaagta ccgcgccacc
    19981 tgcgcctgcc cggcctgcgg cggcacccgc ctgcgcaccg aggcgcgcca cgtcctcgtc
    20041 ggcgagcgca acctgtcgga ggtgagccgc ctgcccctgg cccagtgtcg cgccttcttc
    20101 gacaccctgg ccctcagcgg ccagcgcgcc caggtggccg agaagatcgt cgccgagatc
    20161 gccgcccgcc ttcagttcct catcaacgtg ggactcgact acctcaccct ggaccgctcc
    20221 gccgacaccc tgtccggcgg cgaagcccag cgcatccgcc tcgccagcca gatcggctcc
    20281 ggcctcaccg gcgtcatgta cgtcctcgac gagccctcca tcggcctgca ccagcgcgac
    20341 aacgaccgcc tcctcgacac cctgcggcgc ctgcgcgaca tgggcaacac ggtgatcgtg
    20401 gtggagcacg acgaggacgc catccgcatg gccgactacg tggtggacat gggccccggc
    20461 gccggcgagc acggcgggcg gatcatcgcc gccggccggc ccgaggacat cgccgcccac
    20521 ccggagtccc tcaccggcgc cttcctgtcc ggcacgcgga ccatccccgt gccggccgaa
    20581 cgccgcgcgc ccgagcccga gcgcctgctg cgcatcgagg gcgcctcggg caacaacctg
    20641 cgcgacgtca gcctcgaatt gcccgtcggg ctgttcacct gcgtcaccgg cgtgtccggc
    20701 tccggcaaaa gcaccctcat caacgacacc ctctacgccc acgtggcccg ccacctcaac
    20761 ggcagcagca tggaggccgc gccgtgcacg gcgcttgacg gcctcgacca cttcgacaag
    20821 gtcatcaacg tcgaccagag ccccatcggc cgcacgccgc gttccaaccc ggccacctac
    20881 acgggcctgc tcacgcccat ccgcgaactc tttgccggcg tgcccgacgc caaggcccgc
    20941 ggctacggcc ccggccgctt cagcttcaac gtcaagggcg ggcgctgcga agcctgccag
    21001 ggcgacggcc tgatcaaggt ggagatgcac ttcctgccgg acatgtacgt cccctgcgac
    21061 atctgccacg gcaagcgcta caaccgggaa accctggaaa tccgctacaa gggcaagacc
    21121 atctacgacg tgctggagat gaccgtcgag caggccctgg atttctttgc cccggtgccc
    21181 gtggtcgccc gcaagctcga caccctgatg caggtcggcc tcggctacat ccgcctgggc
    21241 cagagcgcca ccaccctctc cggcggcgag gcccagcgcg tcaagctggc cctcgaactc
    21301 tccaagcgcg acaccggccg caccctctac atcctcgacg aacccaccac cggcctgcac
    21361 ttcgccgaca tcgaactgct cctcagcgtc ctccaccgcc tgcgcgacca cggcaacacg
    21421 gtggtcgtca tcgagcacaa cctggacgtg atcaagaccg ccgactggct catcgacctg
    21481 ggccccgagg gcggcaacgg cgggggcctg atcgtcgccg ccggcacccc ggaagccctc
    21541 gccgcccatc ccgccagcca caccggccgc tacctcgccc gcctcctgaa ggcctgaagc
    21601 ccatgaccga tctctggccg cgtctcgccg cctgcctcga acacctcgcg cccgccgtgc
    21661 cggccgacgc cctggccgaa gccgacaccc accgggacac cctggcgccc cgcctcgtcg
    21721 ccgtgctcga cggcctcgtc gccgacccca cccccgcccg cgccgacggc tatgtgctgc
    21781 acctctacgc catgctgctc ctcgcctgct ggcgcgatgc ccgcgcctac cggcccctgg
    21841 cggccctcgg ccacctggac gagcgcatca tcgacgaagt cttcggccag ctcgtgcacg
    21901 acacctacgg ccgcgccctt gcctccacct gcgacggcga cctggcgccc ctcacggccc
    21961 tcgccgacga ccccgccgcc agccagtggg cccgcgccgc agcgctggag gccctgcttg
    22021 tcgccgccct cgaagggcgc gcagcgcgca ccgccgtcat cgccttcctg gctgacttcg
    22081 gcgcccgcga ggcccaggcc ctgcgcgaca atccggccga cggggagcac ttccagctca
    22141 tcgacgtggt cgccgtgacc gccgccgacc tgggcgccgt cgaactcctg cccgccatcg
    22201 aggcctggta tcgggaaggc ctcatcgaca gcagcttcgc cgaggccgcc gacatcgccg
    22261 ccgacatcgc ccgcagcccg gacgactgcc gcgccgccct gcgccacaag ggccacggcc
    22321 tgattgacga cgtggccgcc gaagtcgccc tgtggcccgc cttccacagc gccccgccgg
    22381 tacgcgggcc ggccctgccc ctcggcctgg tgcgcgaacc tttcgtgcgc gacggcgtca
    22441 aggtgggacg caacgacccc tgcccctgcg gcagcggtcg caaatacaag aagtgccacg
    22501 gcgccgcggt ctgagccgcc ggctgcaaca cccccgcaat cctgccgcca tccggacgcc
    22561 ccacggactc cgctagcctt cgacccaacg cctcctgcca aaccgcccat gaccgacacg
    22621 cccgcaaccg ccgagaaccc ggccttcgac ctggacgact ttgaagtccg cgtcctcgcc
    22681 gtcctcatcg agaaatcctt cgtcacgccc gacatctatc ccctgtccct caacgccctc
    22741 ctcgccggct gcaaccagct caccggccgc gagccggtga tgagcctgtc cgagaccgcc
    22801 gtgcaggacg ccctcgaccg ccttgccgag cggggactcg tcagccagcg ccaccaggcc
    22861 ggcgcgcggg tggccaagtg ggaacaccag atccgcctgc gccattccct caccccgccc
    22921 atgcaggcgg tgctggccct gctgatgctg cgcggccccc agacgccggc cgagatccgc
    22981 gcccgctgcg accgcctgca cgccttcacg tccaccgcgg aggtcgagga gatcctcgaa
    23041 aagctcggcg acaagttccc gcccatggtc gtccagttgc ccaaggcgcc gggcaccaag
    23101 gaagcccgct tcgcccacct gctggccggc gaaggggccg tggccgccct cggtcacacc
    23161 acggaggccg gcgatgcgcc ctcgccgcgg agcggccggg tggccgaact ggaagacgaa
    23221 gtccgccgtc tgcgccagca ggtggactgg ctgaccgccg aattcgaagc cttccgcaag
    23281 cagttccagt aagtccgtcc cgcattcccc gaggcactca tgctcgttca tccccagttc
    23341 aacccggtcg cgctctccat tggtcccctc gacgtgcgct ggtacggcct catgtacctc
    23401 accgccttca tcctcttcgt ggcccttggc cgtgcccatg cccggcgccg gccggaactc
    23461 ggctgggacg cccagcagat cgacgacctg ctgttctacg gggtgctcgg cgtgatcctc
    23521 ggcgggcggc tcggatacat cctgttctac aaaccggcgt actacttctc gcatcccgtc
    23581 gagatgttcg ccgtctggca gggcggcatg gctttccacg gcggcttcct cggcgtgctc
    23641 atcgccgtct ggctgttcgc ccgcagtcac gaccgcccat ggctggcggt cacggacttc
    23701 atcgccccgc tcgtccccct cggcctggcg gcaggacgcc tgggcaactt catcaacggc
    23761 gaactggtcg gccgtgtcac agacgtgccc tgggcgatga tcttcccgca ggtggacatg
    23821 caaccgcgcc acccgtccca gctctaccag ttcaccctcg aaggtgtatg cctgttcgtg
    23881 ctgttgtggg tcttctccgc caagccgcgg ccgcggggtc tggtctcggc ggtgttcctc
    23941 atcggctacg gcggtttccg cttcctggcg gaattcggcc gggagcccga cgacttcctc
    24001 ggcctgctcg ccctcaacct gtccatggga cagtggctgt ccctgccgat gatcgtgatc
    24061 ggcatcgtca tggcggtgat tgccg
//
LOCUS       DAOQMM010000049        24066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_672_length_24066_cov_54.947274, whole genome shotgun sequence.
ACCESSION   DAOQMM010000049 DAOQMM010000000
VERSION     DAOQMM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24066
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_24066_cov_54.947274"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1347
                     /gene="rsmB"
                     /locus_tag="PLH85_09665"
     CDS             28..1347
                     /gene="rsmB"
                     /locus_tag="PLH85_09665"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNB64872.1"
                     /translation="MPISPRRSSPARPLPEDSLAHALCCAAELIARVAGGAALTDILA
                     DLWVSRPATPPAVRGAIQDLTYGTLRDYGRQDFILGRLLDKSLPEPVQSLLRVALHRL
                     DVRPETAHTVVDQAVNAAATLLSGRFKGVVNGVLRNALRQSATLRYAADADLVAEMRH
                     PPWWIRRVRDQHPQDWRNVLAAGNVHPPMALRVNRRRSTPEAAAAELAEAGLSARTWP
                     GGALLLERPVPVVRIPGFAEGRVSVQDIGAQAAAPLLDVASGQHVLDACAAPGGKTAH
                     LLELADVDLLALDADPQRARRIDENLQRLGLSARVMAADCRAIDRWWDGRPFDRILAD
                     VPCSASGVVRRHPDIKWLRRSGDIGRFAAQQAEILDALWQVLAPGGKMLYATCSVFRE
                     ENQDQVARFAASHADCHRLTIEGQTDRQFLPCADHDGFFYALLHKAA"
     gene            1307..1924
                     /locus_tag="PLH85_09670"
     CDS             1307..1924
                     /locus_tag="PLH85_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNB64873.1"
                     /translation="MTDFSTPCSTRLPERRRFLHRLSAVAGGVVLGGLAMPAWTAEAD
                     IRSASLVLTDDLYVLHADIVAELGSLLGDVLMRGVSLFFVVEFQLTSSRWYWLDEEIA
                     NRTLTYRLSYQALTRQYRLSVGALHQNFMTLDEALRTMLRVRNWGVLDRAAVKPGHSY
                     NAAMRFRLDVAELPKPLQVSAFGRPDWNLSSDWLRWTFVPQRDAK"
     gene            1921..4056
                     /locus_tag="PLH85_09675"
     CDS             1921..4056
                     /locus_tag="PLH85_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64874.1"
                     /translation="MRPTALVVAAAVAGILLFLLATASANTPLFARNYTLLLGLNGAI
                     TVGLVALVFVQLRSLWQEHRAHLFGSRLKLRLMAIFAAVAVVPGVLVYAVSLQFAVKS
                     IESWFNVRVDAALDSGMQLGRTTLDFLLDQLAEKGGQMAIELETGGPIRATRLNQLRE
                     QAGVDSATVFAGNGRVQVTSESQMGELVPDYPSPTQLKQARDTRGYRAIEGDAASGLL
                     LRVILPIPSRHLSAEAPLLQVTQEVPRNFAIHAESVQNVYRDYQELSLARNGLKRIYT
                     LTLTLTLLLALFGAVAAAFVLTRRLAAPLLILAEGTEAVAAGDFSPRRALPARDELGV
                     LTQSFNQMTRQLEEARGLAEKNRAAVETSRAYLEGVLANLSAGVLAFSPEGLLRAANH
                     GAMTILNDRLEGCERTPLEAWPAHPMFRDALLRGFHGSDPDWHEQLEVTHADGSTQTL
                     LLHGTRLPADTGGGWVVVFDDITQLIAAQRNAAWAEVARRLAHEIKNPLTPIQLSAER
                     LQFKLAERLDDEGREMLERSTRTIVNQVESMKNLVNAFRDYAKLPAPVLVPLDLNALV
                     REVLVLYEGSRVMIRAELAAELPPVLGDATQIRQVIHNLLQNAEDALVDEPDPEIVLL
                     TKSDARRVDLLLRDNGTGFPPDLLSRAFEPYVTTKSRGTGLGLAIVKKIIDEHSGEIR
                     LANLESGGAEVRIRFRSSESLDHGKNPDR"
     gene            4034..5284
                     /locus_tag="PLH85_09680"
     CDS             4034..5284
                     /locus_tag="PLH85_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB64875.1"
                     /translation="MAKILIVDDEVGIRELLSEILRDEGHDIILAENAAAARAARNAA
                     RPDLVLLDIWMPDTDGVSLLKEWSGNGQLTMPVVMMSGHGTIDTAVEATRIGAMEFLE
                     KPIALQKLLAAVKRGLARVKPTHAPSPLGLAAFSRSPALRDLRKRLEQVAEKSRIILL
                     RVGPGSLAELCARTLQRAGTPWLDLSLTTMPLELNTLESARNGVLYIPELIYLSRPQQ
                     KNLVFAMDRLERHGQTLVVATEHRPEELVEHGWDERVVLRLFEVSLAPPALSEIRDDI
                     PEIAGQILLHYVEAGEVPSRRLSSAALNALRNQNWSGGYSELKAVVKSLALGTLEDEI
                     SMDDVAHLLVKAPSTAFGLPLDLPLREAREMFERLYFEHHLQLDGGNMTRLAEKTGLE
                     RTHLYRKLKQLGISVGRRHEEASS"
     gene            5394..6467
                     /gene="hemE"
                     /locus_tag="PLH85_09685"
     CDS             5394..6467
                     /gene="hemE"
                     /locus_tag="PLH85_09685"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNB64876.1"
                     /translation="MSRPKNDTFLRALLRQPTDYTPVWLMRQAGRYLPEYRETRKRAG
                     SFLDLCKNPSLACEVTLQPLARYNLDAAILFSDILTVPDAMGLGLYFSEGEGPRFERP
                     LKEEWEIRNLAVPDPNLELGYVMDAVSEIRRALDGSVPLIGFSGSPWTLACYMVEGGG
                     SDDYRKIKTLLYTRPDLLHHILDITAQAVTAYLNAQIEAGAQAVMIFDSWGGVLAHDA
                     YKTFSLAYMRRILAGLIREKDGERVPAIVFTKGGGLWLEDIAASGTDAVGLDWTVDIG
                     AARRQVGDRVALQGNLDPSVLFGSPEAVAEEARRVLDAFGPHPGHVFNLGHGISLHTP
                     PDNVSVLVQTVHAHSRALRQQAE"
     gene            6992..9160
                     /locus_tag="PLH85_09690"
     CDS             6992..9160
                     /locus_tag="PLH85_09690"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNB64877.1"
                     /translation="MHVVRVALPVPLPQLFDYTSENVSESDVGRCVRVPFGPAEKNGV
                     ILAIDPPDGITPERLKSVLAIQRDAPALPADWLALVDFAARYYQHPLGEVIALALPPR
                     LRRADAVAGDEADPLLDVTPDGHLALGRSTRESRALAVVRRLAAEGAVRRAALRAAEG
                     AAVADALRRGWLAASAADEPARMATAPELTEEQRAAVARLADGFGRYQPFLLVGVTGS
                     GKTEVYLRLVREAITRGGQALILVPEIALTPQLEARVAGRFPAARVVSLHSGLGEAAR
                     SQGFVQALEGRADIVLGTRLAVFTPLPRLALIVVDEEHDPSYKQQEGVRYSARDLAVW
                     RGRQRGVPVVLGSATPSLESWHAAEQGRYGRVELTRRAIAATLPAVRTLDVRREKLDH
                     GLAPRLLEALDVRLQRGEQSLVFLNRRGYAPVLSCPSCGWVSRCLHCSANMVLHLTDR
                     RLRCHHCSFETGLPVACPDCGDVDIQPFGRGTQRVEEVLTERFPAARVVRVDRDSART
                     RRQWEALLEQIGNGAADILVGTQMMAKGHDFPRLTLVGVLNADASLFAADYRAPERLF
                     QQLMQVGGRAGRGALPGEVLIQTQYPEHPLYQCLAAHDFSRYAALQLAERRQARFPPF
                     AANALLCANAAEVATALDWLKAARAAAEPLAVAHGVHLFDPVPMRMVRLARRERAQLL
                     VEAATRGALQGFLTAWSRLLWSQRPPRDLRWHLDVDPAEV"
     gene            complement(9174..9617)
                     /locus_tag="PLH85_09695"
     CDS             complement(9174..9617)
                     /locus_tag="PLH85_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB64878.1"
                     /translation="MPLTAARIAQLQGMPLFGGLTPRTLEVLLRASRIVDAQAGHVFC
                     EEHAPGDTFFILEAGQVEVSKGGPRGRRVLRQLSAGDCFGEMALMDLSPRSATVRALC
                     ASRALEIPAEALQALYECDVEQFALLMMNMGREVSRRLRSAGTLE"
     gene            9836..10126
                     /locus_tag="PLH85_09700"
     CDS             9836..10126
                     /locus_tag="PLH85_09700"
                     /inference="COORDINATES: protein motif:HMM:NF022160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HNB64879.1"
                     /translation="MRAILLVCFLLALAAAQPVPAADLDLNLRMPGLSLSIGERDRDG
                     RYWDGRQWRDERWWGENCHRYEGHKDFRGHCERRGPGVGRDCPPGQAKKGRC"
     gene            complement(10137..11225)
                     /locus_tag="PLH85_09705"
     CDS             complement(10137..11225)
                     /locus_tag="PLH85_09705"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNB64880.1"
                     /translation="MRESDLKDRALGALMGAFIGDALAVGPHWYYDLDALQRDYGPWI
                     TDYTAPRPDRYHAGLKPGEPSQAGILLAITLRSLVERGGYDEADFCRCLDDEFFPQLD
                     GTPMQGPGGYTSQSIREAWRRRVQDGQPWGRCGGPADTTEAAERIIAIAVRYALEPAE
                     LAAAVTRNTLLTQTDGTVVAMTVAFGAILGLLVEGHPLDGDVSGTLMARVKAGTLPFH
                     TVTQGALQAPAPGAAEAPVAGRFPSPDALLGPAFIARAARDPDIRIEPAWKVSLVYGM
                     PCAVYHQFPAAYYLAARFADDFESAVLHAVNGGGQNQARAILTGALAGAQTGLSGIPA
                     RFIEGLADSARLQAQCARLVGQLVTANR"
     gene            complement(11242..12306)
                     /locus_tag="PLH85_09710"
     CDS             complement(11242..12306)
                     /locus_tag="PLH85_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNB64881.1"
                     /translation="MARSPKLIPALFRLGRTSLRQSTRLGKAVARRGLDDGQTLAKAA
                     RIAMSGVARPAPPPPPRPAGGRWYEGRWGPGPLAMRHYRLFIPTGATARRPLPLLVLL
                     HGCAQDAATFAASTHAARTARDRHFAVLMPEQAREANPQRCWNWFGTPAQVELEVLIL
                     MSLVERVQALYPRCRGPLFALGISAGGAIATTLALHRPTHFTAVGSHSGAAPESAVTP
                     LQAAQAMQGRRSPDLNRVRDLLRGLPPPPLILIHGDADGVVAPGNASATADLWRATLS
                     AGTPLGESRGEIRRGTRLPCQRHDWRHNGRPWLRVITIHGLGHAWSGGAAGFAFSEPR
                     GPDALKLAWQFFAATMEESS"
     gene            complement(12737..13315)
                     /locus_tag="PLH85_09715"
     CDS             complement(12737..13315)
                     /locus_tag="PLH85_09715"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB64882.1"
                     /translation="MSVSPEQLVAVSKANAEASLKTAADFATTLFTAVERMSSLNLSV
                     ARGAFDDTVAFSKAVMAAKDPKEVVALQLGSLPPAVEKGVAYGRSLSEIGGDTKEELT
                     KLAESEAAVLSKNVNTAIEALFKNAPAGSEPAISAVKTAIANATSAYEGASKAAKEVA
                     AAAQATVDKATAVAVESVAKGTKAAASALKAV"
     gene            complement(13479..14039)
                     /locus_tag="PLH85_09720"
     CDS             complement(13479..14039)
                     /locus_tag="PLH85_09720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNB64883.1"
                     /translation="MPTSPCAVPLAHATRLLNHGPTVLVSAAHDGLRNVMAAAWNMPL
                     DFEPPKVAVVIDKNTFTRTLIEASGQFVVAVPTRAQAALTLAIGSRSGRDGDKFVALG
                     LDTLPASRVTAPLPVGCIAWLECRVIPEPHVQQTYDLFLGEVLAAWADPAVFAGGRWQ
                     FDDPATRSIHYVAGGRFLMIGEAFDV"
     gene            14125..14988
                     /locus_tag="PLH85_09725"
     CDS             14125..14988
                     /locus_tag="PLH85_09725"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB64884.1"
                     /translation="MSTLTPSPVRPARRWLRGIAWLAGALLGLAVLAVAGIALRQERL
                     LFHPEPLPAGFDFAAILPPGQTFETIPVRVPGATLSAILLRQQSSRGLVFYLHGNSGN
                     LATWLGNAAFYRALGYDLFLLDYRGFGQSSGVIDGEAQLHDDVRAAWQRVAPAYAGRP
                     IVVIGRSLGSGLAVRLARDVRPDLLVLVTPYASLSRLAAEHMPYVPSALLRYPLASDR
                     IIGDVRSPVLIFHGDRDEVIDVAHAHSLLALAAADAELVEVAGASHTDIQTFAAYRDR
                     LAVRLRALGGP"
     gene            complement(15002..17995)
                     /locus_tag="PLH85_09730"
     CDS             complement(15002..17995)
                     /locus_tag="PLH85_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB64885.1"
                     /translation="MRLARLSLLFSAVLLLSVGMSGLIALQAMQAFDRVQTVQDHRLE
                     ALHAVEALRRDTDTLTRLVRAYTVTGETRFLSYYHALQGIRAGALPVPDDSAPTTYWD
                     RVIAGETPYLPASSGPGMSQRQRMIELGFDARELSALATAMDEAETLKAMEQNAIAAT
                     RSAYNPDNGRFNGGHRFDPGDAVLQVYGQDYDRQRARLTVAIANLLQQVDARTTREVG
                     EARDGLRAWIAGAVAGVAVTAVLVVLGLRTTRRELLHPIEDISAVTGQLARGQYDVRL
                     PEAGHIAEIRALRQAVNSMARAVERDIAEREFIQRELGEARTRAELATQAKSMFLANM
                     SHEIRTPLNAVIGMAELMLHSPLAPRQREYADKIHSAGRTLRDIVNDILDFTKIEAGH
                     LELERIPFRLEGAIANALLPVEQSAAEKGVELIFEATANSARVLDRSFLGDPVRVGQI
                     LGNLLSNAVKFTPSGHVSLRVDGQNNGPQGMRVSITVEDTGIGMRPEQIERLFDDFTQ
                     ADGATTRQFGGTGLGLAIVRRLVDAMGGQIRVHSQPKQGSTFQVVLPLGVDTTTAPRP
                     APELPAEFRVLVVDDTPAARLALIDLLHLSGVDEVDSASDRATALECLDYAVESGFRY
                     DLLLLDDALPGNGTRTLFDDLQQHPARTPRHLALLSTGECPDDSLPETAECPIHRCDK
                     PLLPGALARLFDRVLGRSPARSDVPASGEVGALDGMRVLLVEDNATSRDVAVALMGRW
                     GVEVDSAVDGRDALSQLAAHPPEHYGLVFMDLQMPVMDGYEATRRLRAQPIYAGLPVY
                     ALSAHSGRQVTNRCLALGMNGCLDKPYEVADLYRVLHQYYRGEGRAPGTLPSPSDGRP
                     VLEGLDRIPGLDPRCAINDTGISATLYPRLLQKFRDQFADGPSSLRADVAAHAWDRVA
                     PFAHTLKGRAGLLGMGEISAIAGRLEAAAHAGDFVRAERLLIALEAHLRPLVEGLARA
                     LPASAGEGDPIASTSCPEHSVSAGS"
     gene            complement(18026..18676)
                     /locus_tag="PLH85_09735"
     CDS             complement(18026..18676)
                     /locus_tag="PLH85_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64886.1"
                     /translation="MHGERGVALGIVATIMLGLAADRFGGPWGQPLVSVWTWLFLGAL
                     LWRAPALRRPLLGCLLIATTGEIVLSLVLHLYDYRLGNLPLFVPPGHCLLYWLGLYVA
                     PRLGRHLTTAIPWAAVGGVSILAVSGLDGLGPLLLALFLLCLRYGPAPRLYVSMFALS
                     LLMELWGTALGCWTWRSTLPLVDWPVTNPPLAAGAFYCVLDLLCGSLRHPQTCHSA"
     gene            complement(18687..20318)
                     /locus_tag="PLH85_09740"
     CDS             complement(18687..20318)
                     /locus_tag="PLH85_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HNB64887.1"
                     /translation="MLIANNITMQFGAKPLFENVSIKFGDGNRYGLIGANGAGKSTFM
                     KILAGVLEPSGGNVAIDSGERMAFLRQDQFAYEDVRVLDVVMMGHAEMWACMQEKDAI
                     YANPEATEEDYMKAAELEGHFAEYDGYTAEARAGELLLGVGIPTDKHSGPMREVAPGW
                     KLRVLLAQALFANPDILLLDEPTNNLDINTIRWLEHVLNERNSTMIIISHDRHFLNQV
                     CTHMADLDYGRIQVWPGTWDDYIEASTQARERQAAANQKAKERVQELQEFVRRFSANK
                     SKARQATSRAKLIEKIKIDEFKPSSRQYPWIRFEYDEKEKLHRQAVEIENLSFGYDPA
                     KPIIKNFSFTADANDRIAIIGENGIGKTTLLKLLVGEGLHGLKPQHGTLKWAEKAKLG
                     YYAQDHAEDFAQDENLTDWISGYVREGGYEGDDAETLIRGTLGRLLFKGDEVKKSVKV
                     ISGGEQGRMLFGKLMLQRKNVLLLDEPTNHLDMESIESLNSGLADFTGTLFFVSHDRE
                     FVSSIATRILEIRGPGDIVDYRGTYEEYLASQGIE"
     gene            complement(20454..20867)
                     /locus_tag="PLH85_09745"
     CDS             complement(20454..20867)
                     /locus_tag="PLH85_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNB64888.1"
                     /translation="MSQDPDYSERRTRQRFVARRWGAVCFWVSIDGGERQALNDLSLE
                     GFSYPLDTPPEQPRRFTFSLHLEGIPDTIRGQAETMNHVLGEGGGQLGCRFLSFDDDG
                     ERRLHEWLTTHVMATATVRISEQEAAEIVRGPSLI"
     gene            21159..21812
                     /locus_tag="PLH85_09750"
     CDS             21159..21812
                     /locus_tag="PLH85_09750"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HNB64889.1"
                     /translation="MKKQFLAAAVLAAASFGASAATVSFSDSFGLATTDWTHALTLQQ
                     FDTSLGTLNSVTFSYGGEVSTSFRLESLDAAAANVTANAAGNLVFGGPISNTLAVSGS
                     ASRSLSAFDGAIDFGGTSGAIVGPVVGNKSDVLTLLSGLSVYEGLGTYTINVDARGLS
                     NASGAGNLVSIINTQALANITVTYDYTVTPPGRVPEPASMALVGLGLAGLGAIRRRK"
     gene            complement(21912..22178)
                     /locus_tag="PLH85_09755"
     CDS             complement(21912..22178)
                     /locus_tag="PLH85_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64890.1"
                     /translation="MKKLILSIAVSTFAAGLASSALAADATCAEQAAAKKLAGAAKNS
                     FIKKCEGGGATAPSACEGQAKEKKLHGAAKSSFVKKCEADAAAK"
     gene            complement(22283..23218)
                     /gene="hprK"
                     /locus_tag="PLH85_09760"
     CDS             complement(22283..23218)
                     /gene="hprK"
                     /locus_tag="PLH85_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNB64891.1"
                     /translation="MRQTTVARLFDDNLERLRLRHICGSLETRISVPEDRIWPADMVG
                     HLNLIHPERLQILGSSELMWARRQKREKVVHHVKDILSYRPPALIVADDEDIPSMIRT
                     LCANADVALFATPLPSASIVDQLRGYLSRQLAERIALHGVLMDVLGVGVFITGDSGAG
                     KSELALELISRGHGLVADDIVEFSRIAPNLLVGRCPELLQDVIEVRGLGILNVRTIFG
                     ETACRKKMKLKLVVHLQRRQASQDEPLRLTLEGEEQNILGVPIRRVVLPVAAGRNLAV
                     LLEAAVRSTILLLRGINSTQEFVERQRRAMEDDGY"
     gene            complement(23187..23672)
                     /gene="ptsN"
                     /locus_tag="PLH85_09765"
     CDS             complement(23187..23672)
                     /gene="ptsN"
                     /locus_tag="PLH85_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HNB64892.1"
                     /translation="MNLIAQLLPPANVVVGLEASSKKRAFEQAGLLFENNHGIGRSMV
                     FDSLFAREKLGSTGLGQGIAIPHGRIKGLKDALGAFMRLSEPVQFDAPDGRPVNLLFV
                     LLVPEQATEHHLQLLSELAQMFSDRAFRHQLATAPDAAAVHELFAQWGGDAADHGRPA
                     V"
     gene            complement(23821..>24066)
                     /gene="raiA"
                     /locus_tag="PLH85_09770"
     CDS             complement(23821..>24066)
                     /gene="raiA"
                     /locus_tag="PLH85_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNB64893.1"
                     /translation="VIRHFDNVTSVGVILSVEKLRQKAEVTVHVRGKDIHVESDDADM
                     YAAIDALADKLDRQVLKYKAKTSDHGHDSLKHHVAES"
BASE COUNT         3826 a         7846 c         8239 g         4155 t
ORIGIN      
        1 gtacacgccc tgctcacagc ctgattcatg cccatctccc ccagacgttc atcgcccgcc
       61 cgccccctgc ccgaagacag tctggcccac gcactgtgct gtgccgcaga gctgattgcc
      121 cgtgtcgccg gcggtgctgc cctgaccgat attctcgcgg acctgtgggt gtcgcgccca
      181 gccacaccgc cagcggtccg gggcgcgatt caggatctga cttacggcac gctgcgggat
      241 tacggccgac aggacttcat cctgggtcgc ctgctggaca agtcgctgcc tgaacccgtt
      301 cagtcgctcc tgcgggtcgc gctgcaccgc ctcgatgttc gaccggagac tgcgcatacg
      361 gtggtcgatc aagccgtgaa tgccgcggcc accctccttt cagggcgctt caagggtgtg
      421 gtgaatggtg tgctgcggaa cgcgttgcgc cagtccgcca cgctccgcta cgcggccgat
      481 gccgatctcg tcgccgagat gcggcatccg ccctggtgga ttcgccgtgt gcgggaccag
      541 catccccagg actggcggaa cgtgctcgcc gcggggaacg tacatcctcc gatggcgttg
      601 cgggtcaacc gtcggcgcag caccccggag gccgcggcag ctgaactcgc cgaggccgga
      661 ctgagcgccc ggacctggcc cggtggcgcc cttctcctgg aacgaccggt gccggtcgtc
      721 cgcattccag gtttcgcgga agggcgggtg tccgtgcagg acatcggggc ccaggcggcg
      781 gcaccgctac tggacgtggc cagcggacag cacgtgctcg acgcctgtgc ggcacccggc
      841 ggcaagactg cccacctgct ggaattggcg gacgtggacc tgctggctct ggacgccgat
      901 ccgcaacggg cccgccgcat cgacgagaat ctgcagcgcc tcggtctgag cgcccgtgtc
      961 atggccgcag attgccgtgc gatcgatcgc tggtgggacg gccgtccctt cgaccgcatc
     1021 ctggcggacg tgccctgctc ggcctccggg gtggtccgcc gtcacccgga catcaagtgg
     1081 ctgcgccgct ccggtgacat cggccgcttc gccgcccagc aggccgagat tctcgacgcc
     1141 ctgtggcagg tgctcgcccc aggtggcaaa atgctttacg cgacctgttc ggtgttccgg
     1201 gaggaaaacc aggatcaggt cgcccggttc gcggcttcgc atgcggactg tcatcgcctg
     1261 accatcgagg gccaaaccga tcggcagttc ctgccttgcg ccgaccatga cggatttttc
     1321 tacgccctgc tccacaaggc tgcctgagcg tcgccgcttc ctgcaccgcc tgagcgccgt
     1381 cgccggcggt gtggtgctgg gcggtctggc aatgcccgcg tggacggccg aggccgacat
     1441 ccgttccgcg tccctggtcc tgacggacga cctgtacgtg cttcacgcag acatcgtcgc
     1501 cgagctcggt tcgctgctgg gggacgtact gatgcgtggc gtgtccctgt tcttcgtcgt
     1561 cgagttccag ctcacgtcgt cgcgctggta ctggctcgat gaagagatcg ccaaccgtac
     1621 gctcacctac cgcctgtcct accaggccct cacgcgccag taccgcctgt cggtgggggc
     1681 gctgcaccag aacttcatga ccctggatga ggcgctgcgc accatgctgc gcgtgcgcaa
     1741 ctggggtgtc ctggaccgcg ccgccgtgaa gccggggcac agctacaacg cggccatgcg
     1801 cttccgcctc gatgtggcgg agttgcccaa gcccctgcag gtcagtgcgt tcggccgtcc
     1861 ggactggaac ctgtcctccg attggctgcg ctggaccttt gttccgcaga gggacgccaa
     1921 gtgagaccga cggccctggt cgtcgccgcg gcggtcgcag gcatcctgct gttcctgctg
     1981 gccacggcga gcgccaacac gcccctgttc gcccgcaact acacgttgtt gctggggctc
     2041 aatggcgcca tcacggtagg cttggtcgcg cttgtgttcg tccagttgcg gagcctgtgg
     2101 caagagcacc gggcgcacct gttcgggtcc cggctgaagc tgcgcctgat ggcaatcttc
     2161 gcagccgtgg ccgtggtgcc tggagtgctc gtgtacgcgg tgtcgcttca gtttgcggtc
     2221 aagagcatcg agtcctggtt caatgtgcgc gtcgacgcgg ccctcgacag cgggatgcag
     2281 cttggccgca cgacgctcga cttcctcctc gatcagctcg ccgagaaggg cgggcagatg
     2341 gccatcgagc ttgagaccgg cggtcccata cgcgccacgc gcctgaacca gctgcgggag
     2401 caggccgggg tggacagcgc caccgtattt gccggcaacg ggcgcgtgca ggtgacgtcc
     2461 gagagtcaga tgggcgagct cgtgcccgac tatccgagcc cgacccagct caagcaggcc
     2521 cgcgacacgc gcggctatcg cgccatcgaa ggggacgcgg cgagcggatt gctgctgcgg
     2581 gtgatcctgc cgattccgtc gcgccatctc tccgccgagg cgccgctgct ccaggtgacc
     2641 caggaggtgc cgcgcaattt cgcgatccac gccgagagcg tgcagaacgt ctatcgcgac
     2701 taccaggagc tgtccctggc ccgcaatggc ctcaagcgca tctataccct gacactcacg
     2761 ctgaccctgc tgctggcctt gttcggcgcg gtggcggcgg ccttcgtgct gacccgccgc
     2821 ctggcggcgc cgctgctgat cctggctgaa gggaccgagg ccgtggcggc cggggacttc
     2881 agccctcggc gggccctgcc ggcgcgcgac gaactgggcg tcctgaccca gtccttcaac
     2941 cagatgaccc gccagctcga ggaggcgcgc ggcctggccg agaagaatcg ggcggccgtc
     3001 gagaccagcc gcgcctacct tgaaggcgtg ctggccaacc tgtccgccgg cgtgcttgcg
     3061 ttctctcccg agggcctgtt gcgggccgcc aatcatggcg cgatgaccat cctcaacgat
     3121 cgcctggaag gctgcgagcg cacgccgctg gaggcctggc ctgcgcatcc gatgttccgc
     3181 gacgcgctgc tacggggctt tcatggcagc gatccggact ggcacgagca actggaagtg
     3241 acccacgcgg acggcagcac ccagacactg ctcctccatg gcacgcgttt gccagctgac
     3301 accggtggcg gctgggtggt ggtcttcgac gacatcaccc agctcatcgc cgcccagcgc
     3361 aacgcggcct gggccgaggt ggcgcggcgc cttgcccacg agatcaagaa cccgctgaca
     3421 cccatccagc tttctgccga gcgcctgcag ttcaagctgg cggagcgcct cgacgacgaa
     3481 ggccgcgaga tgctggagcg ctccacccgg accatcgtca atcaggtgga gtccatgaag
     3541 aacctggtga acgcctttcg cgattacgcg aagctgccgg cgccggtgct tgttcccctc
     3601 gatctcaacg ccctggtacg ggaagtgctc gtgctttacg aaggttcgcg ggtcatgatc
     3661 cgcgccgaac tggctgccga acttccaccg gtgctgggcg acgcgaccca gatccgccag
     3721 gtcatccaca atctgctgca gaacgccgag gacgccctgg tggacgaacc ggaccccgaa
     3781 atcgtgctgt tgaccaagtc cgacgcccgc cgggtagact tgctgctgcg cgacaacggc
     3841 accggctttc cgccggatct gctgtcccgt gccttcgagc cctacgtgac gaccaagtcg
     3901 cgggggaccg gtcttggctt ggcgatcgtc aagaaaatca tcgatgaaca cagtggcgag
     3961 atccgtctcg ccaatctgga atcgggcggc gccgaggtgc gcatccgctt ccgctcaagc
     4021 gagtcactgg accatggcaa aaatcctgat cgttgacgac gaagtcggca tccgcgaact
     4081 cctctcggag atcctgcgcg atgaaggcca cgacatcatc ctcgccgaaa acgccgctgc
     4141 cgcccgggcc gcccgcaatg cagcgcgccc cgatctggtg ctcctcgata tctggatgcc
     4201 cgacacggat ggtgtcagcc tgctcaagga atggtccggc aatggccagc tcaccatgcc
     4261 agtggtgatg atgtccggcc acggcaccat cgacaccgcg gtggaagcga cccgcatcgg
     4321 cgcgatggag ttcctcgaaa agccgattgc cctgcaaaag ctgctggcgg cggtgaagcg
     4381 tggcctcgcg cgggtcaagc cgacccacgc gcccagtccg ctgggactgg cagccttctc
     4441 gcgttcgccc gccctgcgcg atctgcgcaa gcgcctcgag caggtggccg agaagtcccg
     4501 catcatcctg ctgcgggtcg gtcccggcag tctcgccgaa ctgtgcgcgc gcaccctgca
     4561 gcgggccggc acgccgtggc tggatctgtc cctgaccacc atgccgctgg agctcaatac
     4621 ccttgagagc gcccgcaacg gcgtgctcta tattcccgaa ctgatctacc tgtcgcgtcc
     4681 ccagcagaag aatctggtgt tcgccatgga tcgcctggaa cgtcacggtc agacgctcgt
     4741 ggtggccacc gagcatcgcc cggaggagct tgtcgaacac ggctgggacg aaagggtggt
     4801 gctgcgcctg ttcgaagttt ccctcgcgcc gccggccttg tccgagatcc gcgacgacat
     4861 tccggagatc gccggccaga tcctgctgca ttacgtggag gccggcgagg tcccgtcgag
     4921 gcgcctttcg tcggcagcgc tcaatgcctt gcgcaatcag aactggagcg gcggttacag
     4981 cgagctcaag gcagtggtga agtccctcgc cctgggcacg ctggaagacg agatttccat
     5041 ggacgacgtg gcccatctgc tcgtcaaggc gccgtccacg gccttcgggc tgcccctcga
     5101 cctgcccctg cgcgaggccc gcgaaatgtt cgaacgtctc tacttcgagc atcatctgca
     5161 actggacggc ggcaacatga cccggctcgc cgaaaagacc ggcctggagc gcacccacct
     5221 ctaccgcaag ctcaagcagc tgggcatcag cgtagggcgg cgtcacgagg aagcgagttc
     5281 ctgaccggcc cgcgacggtt gccggcgatt gatccagccc atcgccgccg gacgggcaaa
     5341 tgacggataa tcccgccttt gccgcactgg cggcacgctg ccgaggatac gacgtgagcc
     5401 gtcccaagaa cgataccttc ctgcgcgccc tgctgcgcca gcccaccgac tacacgcccg
     5461 tctggctgat gcgtcaggct ggccgctatc tgcccgaata ccgcgagacc cgcaagcgtg
     5521 ccggcagctt cctcgatctc tgcaagaacc ccagcctggc ctgcgaagtc accctgcagc
     5581 ctctcgcccg ctacaacctg gacgcggcca tcctcttctc cgacatcctc accgtgccgg
     5641 acgccatggg cctgggcctg tatttctcag aaggcgaagg cccgcgcttc gagcgcccgc
     5701 tcaaggagga atgggagatc cgcaacctgg cagtgcccga tcccaacctg gaactgggct
     5761 acgtgatgga cgcagtctcc gagatccgcc gcgccctcga cggctcggtg ccgctgatcg
     5821 gcttctccgg cagtccgtgg accttggcct gttacatggt ggaagggggt ggctcggacg
     5881 actaccgcaa gatcaagacc ctgctctaca cccggccgga cctgctgcat cacattctgg
     5941 acatcaccgc ccaggccgtc accgcctatc tcaatgcgca gatcgaagcc ggcgcccagg
     6001 ctgtgatgat cttcgattcc tggggtggcg tgctggccca cgacgcctac aagacctttt
     6061 cgctggccta catgcggcgc atcctcgcgg gcctgatccg tgagaaggac ggcgaacgcg
     6121 tgcccgccat cgtcttcacc aagggtggcg ggttgtggct ggaagacatc gccgccagtg
     6181 gcaccgacgc ggtcggactg gactggaccg tcgatatcgg ggcagcccgc cggcaggttg
     6241 gcgaccgggt ggcgctgcag ggtaatctgg atccttcggt gctgttcggc agccctgaag
     6301 ccgtggcgga ggaagcacgc agggttctgg acgcgttcgg cccccatccg ggccacgtgt
     6361 tcaacctggg ccatggcatt tccctgcata cgccgccgga caatgtctcg gtcctggtgc
     6421 agacggtgca cgcccatagc cgggcgctgc ggcagcaggc cgagtgagat cccggaacgt
     6481 ggtccgaggc cggcgttgcc ggcctttttt gttgagcacg tcgcaggggc cggccttgca
     6541 gccgaaccgt atttggcgaa ttcgcacgcc gcgggcgaat gcacggagat ggaatgacaa
     6601 tggcgtgggt ccggcagctt gcccggagcg ggacgcgtca agcacttttt ttgcgcgagc
     6661 ggccttgact tgtgcacagc cgcatgattc ccgccagaca gccctcacca aaattgaggt
     6721 gatccggcgt atgtttataa agcattgtat ttaaacgatt aaaattttgc ctaggaaatg
     6781 agcagggtag gatgaagcct gttggcaggc gggtttggca ggttttcaca ggggtttccc
     6841 acaaagttat ccacagaatg tgtggacaat gggaaatacc gcgaccgggc aagcatttca
     6901 gggatatttc agaagcgttg cgtgatgaat gcggcgcaaa ttgctgattt atatggaaat
     6961 aaacaagatt taacaacatt gagaaattgc cgtgcatgtc gtccgagtgg cacttcccgt
     7021 cccgcttccg caactgtttg attacacatc tgaaaatgtg tccgaatcgg atgtggggcg
     7081 gtgtgtgcgg gttcccttcg gacccgccga gaaaaacggc gtcatccttg cgatcgaccc
     7141 gccggatggg atcacgccgg agcgtctcaa atccgtcctg gccatccagc gcgacgcgcc
     7201 tgccctgccc gcggactggc tggcgctggt ggacttcgcg gcccgctact accagcaccc
     7261 cctcggtgaa gtgattgccc ttgccctgcc gcctcgcctg cgtcgcgcgg atgccgtcgc
     7321 cggcgatgaa gcggacccct tgctcgacgt gacgccggac ggccaccttg ccttggggcg
     7381 tagcacgcgc gaaagccgtg ccctggcggt ggtccgccgg ctggccgcgg agggcgcggt
     7441 ccggcgtgct gcattgcggg cggccgaggg agcggctgtc gccgatgccc tgcgccgggg
     7501 gtggcttgcc gcctccgccg cagacgaacc ggcgcgcatg gccaccgcgc ctgagctcac
     7561 ggaggaacag cgcgccgccg tggcgcgtct cgccgacgga ttcgggcgct accagccctt
     7621 tctgctggtc ggcgtgaccg gcagtggcaa aaccgaggtg tatctgcgcc tcgtccgcga
     7681 ggcgatcacc cgcggcgggc aggcgctgat cctggtgccc gagatcgcgc tcacgcccca
     7741 gctcgaagcc cgcgtggccg ggcgctttcc cgctgcgcgg gtggtgagcc tgcattccgg
     7801 actgggcgag gcggcccgct cgcagggctt cgtgcaggcc ctggaaggcc gtgccgacat
     7861 tgtcctgggc acgcgcctcg ccgtgttcac gcccctgccc cgcctcgccc tgatcgtcgt
     7921 ggatgaggaa cacgatccgt cctacaagca gcaggaaggc gtgcgctact cggcccgcga
     7981 cctcgcggtg tggcgcggcc ggcaacgcgg cgtgccggtc gtgctgggct cggccacgcc
     8041 ttccctcgaa agctggcacg cggccgagca ggggcgctat ggccgtgtgg aactgacgcg
     8101 ccgggcgatt gcggccaccc tgcctgccgt gcgcaccctc gacgtgcgcc gcgagaagct
     8161 ggatcacggg ctggccccgc gcctgctgga ggcgctcgat gtccgtctgc agcggggtga
     8221 gcagagcctg gtgttcctca accggcgggg ctatgccccg gtcctgtcgt gtccttcctg
     8281 tggctgggtg agccgctgcc tccattgctc tgccaacatg gtgctgcacc tgaccgaccg
     8341 ccgcctgcgc tgccatcact gcagcttcga gacaggtctg ccggtggcgt gcccggactg
     8401 cggcgacgtg gacatccagc ccttcgggcg cggtacccag cgggtcgaag aggtactgac
     8461 cgagcgcttc cccgccgcgc gggtggtgcg cgtggaccgc gattcggcgc gtacccggcg
     8521 ccaatgggaa gctctgctgg aacagatcgg caatggggct gcggacattc tggtgggcac
     8581 ccagatgatg gcgaagggcc atgactttcc gcgcctgacc ctggtgggcg tcctgaatgc
     8641 cgatgcgtcc ctgtttgccg ccgattaccg tgcgccggag cgcctcttcc agcagctcat
     8701 gcaggtcggt gggcgggcgg gccggggtgc actgcccggc gaggtgttga tccagacgca
     8761 gtatccagag cacccgctct atcagtgcct cgctgcccat gacttcagcc gctacgccgc
     8821 gctgcagctg gcggaacgtc gtcaggcccg ctttcccccg tttgccgcca atgccctgtt
     8881 gtgtgccaat gcggccgagg tggcgacggc gctcgactgg ctcaaggccg ctcgcgccgc
     8941 tgccgagccc ctggcggtgg cccacggcgt gcacctcttc gaccccgtac cgatgcgcat
     9001 ggtgcgcctg gcacgccggg agcgggccca gctgctggtg gaggccgcca cgcggggtgc
     9061 gttgcagggc tttctcacgg cgtggtcgcg cctgctgtgg tcacaacggc ccccccgcga
     9121 cctacgctgg catctggatg tggacccggc cgaagtctga gcgcaggcgc cgctcactcc
     9181 agcgtacccg cagagcgtag ccggcggctg acctcgcgcc ccatgttcat catcaggagg
     9241 gcgaactgct cgacgtcgca ttcgtagagc gcctgcagcg cctcggcggg gatctccagg
     9301 gcgcgactgg cgcagagggc gcgtaccgtg gcgctgcgcg gcgaaagatc catcagggcc
     9361 atttctccaa agcagtcccc cgcactgagc tggcgcagga cccgccgccc ccgcgggccg
     9421 cccttgctca cttcgacctg cccggcctcg aggatgaaga aggtgtcacc gggcgcgtgc
     9481 tcttcgcaga atacatgtcc cgcctgggcg tccacgattc gcgaggcccg tagcagaacc
     9541 tccagtgtcc ggggggtgag accgccgaag aggggcatcc cttgcaattg agcgatgcgg
     9601 gcggcggtga gcggcacggt ggattccttc ggggggctgg ccgatgggcc ggagatatcc
     9661 tgtttatagc gcgtgcgccc ggcattgaca cgtcgtcacg gaggccgtgc gcccgcggca
     9721 tgttctgcac actgtgaaaa agtacgacgt cggatgcgaa atagtgcgca gcgtgaccgc
     9781 atgctccggt gctatcgtga gcccgtattc caatcgcctt tcccggggcc tcgtcatgcg
     9841 tgccattctg ctcgtctgct ttctgctcgc cctcgcggcc gcccagcccg tgcctgccgc
     9901 cgatctggac ctcaacctgc gcatgcccgg cttgagcctt tccatcggag agcgcgaccg
     9961 ggatgggcgc tactgggatg ggcggcagtg gcgggacgaa cggtggtggg gcgagaactg
    10021 ccatcgttac gagggccaca aggacttccg cggccactgc gagcgtcgcg gtccgggtgt
    10081 cggtcgtgac tgtccgccgg gacaggccaa gaagggccgg tgctgagcgt cgccgctcaa
    10141 cggttcgccg ttaccagctg tcccaccagc cgcgcgcact gcgcctgcag ccgcgccgaa
    10201 tctgccaggc cctcgatgaa gcgggcggga atgcccgaca ggcccgtctg cgcacctgcg
    10261 agggcgcccg tgaggatcgc ccgcgcctga ttctgaccgc cgccattgac tgcatgcagg
    10321 acggccgatt cgaagtcgtc cgcgaagcgg gccgccagat agtaggccgc cggaaactgg
    10381 tgatacaccg cgcacggcat gccatacacc agcgaaacct tccaggctgg ttcgatacgg
    10441 atgtccggat cgcgggccgc gcgcgcgatg aaagccgggc ccagcagcgc gtcgggtgag
    10501 ggaaagcgtc cggccaccgg tgcttcggcg gcgccgggtg cgggcgcctg aagggccccc
    10561 tgggtgaccg tatggaaggg caaggtgccc gccttgaccc gcgccatcag cgtgcccgac
    10621 acgtccccgt ccaggggatg tccctccacc agcagtccca ggatggcccc aaaggcgacg
    10681 gtcatcgcca ccacagtgcc gtcggtctgg gtcaggagcg tattgcgcgt cacggccgcc
    10741 gcgagctccg cgggttcgag ggcatagcga actgcgatgg cgatgatccg ctcggcggcc
    10801 tccgtggtat cggccggtcc gccgcagcgt ccccagggct ggccgtcctg cacgcggcgg
    10861 cgccaggcct cgcggatcga ctgactggtg tagccgcccg gtccctgcat gggggtcccg
    10921 tccagctggg ggaaaaactc gtcgtccagg cagcggcaga agtccgcctc gtcgtagccg
    10981 ccccgctcca ccagcgagcg cagggtgatg gccagcagga tgccggcctg cgagggttcg
    11041 ccaggtttca ggccggcgtg atagcgatcc gggcgcggcg cggtgtaatc ggtgatccac
    11101 gggccgtaat cgcgctggag ggcatccagg tcgtaatacc agtgagggcc aaccgccagt
    11161 gcgtcgccaa tgaacgcccc catgagcgcc ccgagggcgc ggtctttgag atcggattca
    11221 cgcatgagtg gactttcgtg gtcaggacga ttcttccatc gtagccgcaa aaaactgcca
    11281 cgccagcttc agcgcgtccg gtccgcgggg ctccgagaag gcgaagccgg cggcgccacc
    11341 gctccaggcg tgacccagcc cgtggatggt gatcacgcgc agccaggggc gcccattgtg
    11401 gcgccagtcg tgacgctggc agggcaggcg cgtaccgcgg cggatctcgc cccgcgactc
    11461 gccaagcggc gtgccggcgg acagtgtggc ccgccacagg tcagcggtgg cggaggcatt
    11521 gccgggtgcg acgacgccgt cggcatcgcc atggatcagg atcagggggg gcggcggaag
    11581 cccccgcagc aggtcgcgga cccggttcag atcggggctc cgccggccct gcatcgcctg
    11641 agcggcctgg aggggcgtga ctgcgctctc cggtgcggcg ccggaatggc tgccgacggc
    11701 ggtgaagtgt gtcggccggt ggagggcgag ggtcgtggcg atggcacccc cggcagagat
    11761 gcccagcgca aagagggggc cccggcaccg cggatagagc gcctgcaccc gctcgacgag
    11821 actcatcagg atgagcactt ccagctccac ctgagcaggt gtgccaaacc agttccagca
    11881 gcgctgcgga ttggcttccc gcgcctgctc gggcatcagc accgcaaagt gccgatcgcg
    11941 cgccgtccgt gcggcgtggg tggaggccgc gaaggtggcg gcatcctggg cacagccatg
    12001 cagcaggacc agcagtggca gggggcggcg ggccgtggcg ccagtgggaa tgaacagccg
    12061 gtaatggcgc atggccagcg gacccggtcc ccagcggcct tcgtaccagc gaccgccggc
    12121 cgggcgcggc ggtggcggtg gtgccggtcg ggcgaccccg ctcatggcga tgcgggccgc
    12181 cttggccagc gtctggccgt catccaggcc acgtcgggcg accgccttgc ccagccgggt
    12241 cgactggcgc aggctggtac gcccgagacg gaacagggcg gggatcagct tcgggctgcg
    12301 ggccatggcg tcggtgatgc tcctgtggca ggcggttcaa gggcctcggg aggattgtgg
    12361 cggcaaggca aagaccgcgt ccagtgcggc gctgcgtgaa gcggggggag gatggggccg
    12421 cgccaggacg gcgcgcgatc catgcgtcgg tctggccgac ctgtatccgg cgctcgtaga
    12481 ggcctgtccg gcggcgggag cgggtgtcgg tgccgcgcag gggcgacgat gactgcgttt
    12541 tacgggacgg ccagcactgt ctgtgcgggc tgcgatacag gcctgcgccg cgggccgctg
    12601 acgggtgagt gagggtgagt tcgtcgtctg cagaggcgtt gcgggcgacc tcggttgcag
    12661 gcgtgccaaa ggaaaaggcc ccgcagggcc ttttccggta cgacaggcaa gcgcgggcgg
    12721 gccgcgccag gccggatcag acggccttca gggcggaggc ggcagccttg gtgcccttgg
    12781 ccacgctttc cacggccacg gcggtcgcct tgtcgacggt ggcctgggcg gcggcggcca
    12841 cttccttggc agccttggag gcgccttcgt aggcggaggt cgcgttggcg atggccgtct
    12901 tgaccgcgga aatggcgggt tcggaaccgg ccggcgcgtt cttgaagagg gcctcgatgg
    12961 cggtattcac gttcttggac agaacagccg cttcgctttc ggcgagcttg gtgagttctt
    13021 ccttggtgtc gccgccgatt tcggacaggc tgcggccgta ggccacgccc ttttcaacgg
    13081 cgggcggcag ggagcccagc tgcagggcga ccacttcctt cggatccttg gcggccatca
    13141 ccgccttgga gaaggcgacg gtgtcgtcaa aggcgccgcg ggcgacggac aggttcaggc
    13201 tggacatgcg ttcgacagcg gtgaacaggg tggtggcgaa atcggcagcg gtcttcaggc
    13261 tggcttcggc gttggccttg ctgacggcga cgagttgttc ggggctcacg gacatgatga
    13321 atctcctgga aaggttggga tcgcagaacc cgaatgaatt cggtatggct ctatgttgca
    13381 ctgcacaaaa cagaaacgca agcgattttt gttgcggtgc acaaaatatt ctcgtcggca
    13441 ccgtttcccg ctccgaaccc ccgaagcggc gacctccttc aaacgtcgaa ggcttccccg
    13501 atcatcaaga aacgaccgcc ggccacataa tggatgctac gtgttgcagg atcgtcaaat
    13561 tgccagcgtc cgcccgcaaa aacggcggga tccgcccagg cggcgagcac ttcgccgagg
    13621 aacaggtcgt aggtctgctg gacgtggggt tccgggatga cgcggcactc cagccaggcg
    13681 atgcagccca ccggcagcgg tgccgtcacg cggctcgctg gcagggtgtc gagacccagg
    13741 gcgacaaact tgtcgccgtc ccgcccgctg cggctgccga tggccagcgt gagcgctgcc
    13801 tgggcccgcg tcgggacggc gaccacgaat tgccccgacg cttcgatcag ggtgcgggtg
    13861 aaggtgtttt tgtcgatgac gactgccact ttgggcggtt cgaaatcgag aggcatgttc
    13921 caggcggccg ccatgacgtt gcgaaggccg tcgtgggccg ccgagaccag cacggtggga
    13981 ccgtgattga ggagacgtgt ggcgtgggcg aggggaacgg cgcagggcga cgtgggcatc
    14041 ggcgtgagag ggtgtgcatg agcatacagt gtagcgcggc ggcgtcgcgt gcatccggcc
    14101 ggcgctcacc tgcgtagacc gcgcatgagc accctgacgc cctcacccgt gcgcccggcg
    14161 cgacgctggc tgcgtggcat tgcatggctc gccggcgcac tgctcggcct tgccgtcctc
    14221 gcggtggcgg gcatcgcgct gcggcaggag cgcctgctct tccatccgga gccgctccct
    14281 gcaggcttcg acttcgccgc catcctgccg ccgggccaga cattcgagac gattccggtg
    14341 cgtgtacccg gcgcgacgct gtcggccatc ctgttacgcc aacagagttc ccgtggtctg
    14401 gtgttctacc tccatggcaa cagcggcaat ctggccacct ggcttggtaa tgccgcgttc
    14461 taccgggccc tgggctacga cctcttcctg ctggattacc ggggattcgg acaaagcagc
    14521 ggcgtgatcg atggcgaggc gcaattacat gacgatgtgc gggccgcgtg gcagcgcgtt
    14581 gcgccggctt acgcgggccg gcccatcgtc gtgatcggac ggtcactggg cagcggcctg
    14641 gcggtacggc tcgcccgcga cgtgcggccg gatctgctgg tgctggtgac gccttacgcg
    14701 agcctgtcgc gtctggcggc cgaacacatg ccctacgtgc cgtcggcgct gctccgctat
    14761 cccctcgcca gcgaccggat catcggcgac gtgcggagcc cggtgttgat cttccacggc
    14821 gaccgggacg aagtgatcga tgtcgcgcat gcccacagtt tgctagcgct ggcggccgcg
    14881 gacgccgaac tggttgaggt ggccggcgcc agtcacacgg acatccagac cttcgcggcc
    14941 taccgggacc gcctggccgt acggcttcgt gcgctgggcg ggccttaggg cgcccgccga
    15001 ctcaggatcc ggcggacacg gagtgttccg gacacgacgt cgaggcgatg gggtcccctt
    15061 cgcccgcgga tgccggcagg gcgcgcgcga ggccctcgac caggggacgc aggtgcgcct
    15121 ccagggcgat cagcagacgt tctgcgcgga cgaaatcacc cgcgtgggcg gcagcctcca
    15181 ggcgccccgc aatggccgag atctccccca tgccgagcag cccggcgcgt cccttgagcg
    15241 tgtgggcgaa cggtgcgacg cggtcccagg cgtgggccgc cacgtcggcc cgcaggctgc
    15301 tggggccatc cgcgaactgg tcccggaact tctggagcag gcgcgggtag agggtcgcgc
    15361 tgataccggt gtcgttgatc gcacagcgtg ggtcgagccc gggaatgcga tcgagccctt
    15421 ccaggaccgg gcgtccgtcg gagggcgagg gcagcgtccc cggcgcgcgc ccctcgccgc
    15481 ggtaatactg atgcaggacg cggtacaggt ccgccacctc gtaaggtttg tcgaggcagc
    15541 cgttcatgcc cagcgccaga catcggttgg tgacctggcg cccgctgtgg gccgataggg
    15601 cgtagaccgg cagccctgca tagatcggct gggcgcgcag gcggcgagtg gcctcgtagc
    15661 cgtccatcac gggcatctgc aggtccatga agaccagccc atagtgctcg ggcgggtggg
    15721 cggcgagctg ggacagggcg tcgcggccat ccacggcgct gtccacttcc accccccagc
    15781 ggcccatcag ggcgacggcc acgtcccggc tggtggcgtt gtcctccacg aggaggacgc
    15841 gcattccgtc gagggcgccc acttcgccgg acgccgggac gtccgatctt gcagggctac
    15901 gtccgagtac acgatcgaac aggcgtgcca gcgcgcccgg cagcaggggc ttgtcgcagc
    15961 gatggatcgg acattcggcg gtttcgggca ggctgtcgtc cgggcattcg ccggtggaca
    16021 gcagggccag atggcgtggc gtgcgtgcgg gatgctgctg caggtcgtcg aagagggtgc
    16081 gcgtgccgtt tcccggcaat gcatcgtcga gcaggaggag gtcgtagcgg aagccggatt
    16141 cgacagcgta gtccaggcat tccagcgccg tggcccggtc gctggcggag tccacctcgt
    16201 cgacgccgct gagatggagc aggtcgatga gggcgaggcg cgccgccggc gtgtcgtcca
    16261 ccaccagcac gcggaattcc gccggcagct cgggcgctgg tcgcggcgcc gtggtggtgt
    16321 cgacgccgag gggcaggacg acctggaagg tgctgccctg cttgggctgg ctgtggaccc
    16381 ggatctggcc gcccatggca tccaccaggc gccggacgat ggcaagaccc aggccggtgc
    16441 cgccgaactg gcgtgtggtg gctccgtcgg cctgggtgaa gtcgtcgaac aggcgctcga
    16501 tctgctccgg acgcatgccg atgcccgtgt cttccacggt gatgctgacc cgcatgccct
    16561 gcgggccgtt gttctgccca tccacgcgca gactgacgtg gcccgagggc gtgaacttga
    16621 cggcgttgga caggaggttg ccgaggatct gccccacccg gaccggatcg cccagaaagg
    16681 atcggtcgag gacccgggcg ctgttggcgg tggcttcgaa gatcagctcg acgccctttt
    16741 cggccgcgga ctgctcgacc ggaagcagcg cgttggcgat ggcaccttcc aggcggaagg
    16801 ggatgcgttc gagctccaga tgcccggcct cgatcttggt gaagtcgagg atgtcgttga
    16861 cgatgtcccg cagggtacgg ccagcgctgt ggatcttgtc ggcgtactcc cgctggcgcg
    16921 gcgccagggg actgtgcagc atcagctcgg ccatgccgat cacggcattg agcggcgtgc
    16981 ggatctcgtg gctcatgttg gccaggaaca tggacttggc ctgggtcgcc agttcggcac
    17041 gggtgcgtgc ctctcccagc tcgcgctgga tgaactcccg ctcggcgatg tcccgttcga
    17101 ccgcacgggc catgctgttg accgcctggc gcagggcgcg gatttccgcg atgtgcccgg
    17161 cttcgggcag gcgcacatcg tattggccgc gggcaagctg accggtgacg gcactgatgt
    17221 cctcgatggg atggagcagc tcgcggcggg tcgtgcgcag gcccaggacc accagcacgg
    17281 ccgtgacggc gacgcccgcc acggcgccgg cgatccaggc gcgcaagccg tcgcgcgcct
    17341 cgcccacctc ccgggtggtc cgtgcatcca cctgctgaag cagattggcg atggccaccg
    17401 tgaggcgggc ccgttgccgg tcgtagtcct ggccgtagac ctgcaggacg gcatcaccgg
    17461 gatcgaagcg atgcccgccg ttgaagcgcc cgttgtccgg gttgtaggcg ctgcgcgtcg
    17521 ccgcgatggc gttctgctcc attgccttga gggtctccgc ctcgtccatg gcggtggcca
    17581 gggcggacag ctcgcgggcg tcgaagccca gttcgatcat gcgctggcgc tgggacatcc
    17641 ctggaccgga cgacgcgggc aggtaaggcg tttcgcccgc gatgacccga tcccagtagg
    17701 tggtgggggc cgaatcgtcc ggtacgggca gcgcacctgc gcggattccc tggagagcgt
    17761 ggtaatagct caggaagcgg gtctcgcccg tgacggtgta agcgcgcacg aggcgggtca
    17821 gggtgtcggt gtcgcggcgc agggcctcca cggcatgcag cgcttcgagg cggtggtcct
    17881 gcacggtctg gacgcgatcg aacgcctgca tggcctgcag ggcgatgagg ccgctcatgc
    17941 cgacggacag cagcaggaca gctgaaaaca ggagggaaag ccgggccagt ctcatggcga
    18001 cggcaagctt acaccctcgc cgatgtcatg cactgtgaca ggtttgggga tgacgcagag
    18061 aaccgcacag cagatcgagc acgcaataga aggcaccggc agcgaggggc gggttggtga
    18121 ccggccagtc caccagcggc agcgtgctgc gccaggtcca gcagcccagt gccgtccccc
    18181 agagttccat cagcagtgac aaggcgaaca tgctcacgta caggcgtgga gcaggtccat
    18241 aacgcaggca cagcaggaac agggcaagca ggagcgggcc gaggccgtcc aggccgctca
    18301 ccgccaggat gctgacgccc cccaccgcgg cccaggggat ggctgtcgtg aggtgtcgcc
    18361 ccaagcgcgg tgcgacgtac agccccagcc agtagagcag gcagtgtccc ggaggaacga
    18421 agaggggcag gttgccgagg cgataatcgt agaggtgcag gaccagggag agcacgattt
    18481 ccccggtcgt ggcgatcagc aggcagccca gcagcggcct gcgtaaggcc ggcgcgcgcc
    18541 acaggagcgc tccaaggaac agccaggtcc acacgctgac caggggctga ccccaggggc
    18601 cgccgaaacg gtcggccgcc aggcccagca tgatcgtggc gacgatcccc agcgcgacgc
    18661 cccgctcccc gtgcatggcg gcgggcttac tcgatgccct ggctggccag gtattcctcg
    18721 taggtgccgc ggtaatccac gatgtcgcca gggccgcgga tttccaggat gcgggtggcg
    18781 atggacgaca cgaattcccg gtcgtgggag acgaagaaca gggtgccggt gaaatcggcg
    18841 aggcccgagt tgagggactc gatggattcc atgtccaggt ggttggtcgg ttcgtccagc
    18901 agcagcacgt tcttgcgctg gagcatcagc ttgccgaaga gcatgcgccc ctgttcgccg
    18961 ccggagatga ccttgaccga cttcttcacc tcgtcaccct tgaagagcag gcgtccgagg
    19021 gtgccgcgga tcagggtttc ggcgtcgtcg ccctcgtagc cgccctcgcg cacgtagccg
    19081 ctgatccagt cggtcaggtt ctcgtcctgg gcgaagtctt cggcgtgatc ctgagcgtag
    19141 tagcccagtt tggccttctc ggcccacttg agggtgccgt gttggggctt caagccgtgc
    19201 aggccctcgc ccaccagcag cttgagcagg gtggtcttgc cgatgccgtt ctcgccaatg
    19261 atggcgatgc ggtcgttggc gtcagcggtg aagctgaagt tcttgatgat cggcttggcc
    19321 gggtcgtagc cgaagctgag gttctcgatc tccacggcct ggcggtgcag cttttccttt
    19381 tcgtcgtact cgaagcggat ccacggatac tgacgactgg agggcttgaa ctcgtcgatc
    19441 ttgatcttct cgatgagctt ggcgcggctg gtggcctggc gcgccttgga tttgttggcg
    19501 ctgaaacggc gcacgaattc ctgcagttcc tggacccgct ccttggcctt ctggttggcc
    19561 gcggcctgac gctcgcgggc ctgggtggag gcttcgatgt agtcgtccca ggtgcccggc
    19621 cacacctgga tgcggccgta gtccaggtcc gccatgtggg tgcacacctg gttcaggaag
    19681 tggcggtcgt gggagatgat gatcatcgtg gagttgcgct cgttgagcac gtgctccagc
    19741 cagcggatgg tgttgatgtc caggttgttg gtcggttcgt ccagcagcag gatgtccggg
    19801 ttggcgaaca gggcctgggc cagcagcacg cgcagcttcc agccgggggc cacctcgcgc
    19861 atcgggccgc tgtgcttgtc ggtcgggatg ccgaccccca gcagcagttc gccggcgcgg
    19921 gcctcggcgg tgtagccgtc gtattcggcg aagtggcctt ccaactcggc cgccttcatg
    19981 tagtcctctt cggtggcctc cgggttggcg tagatggcgt ccttttcctg catgcaggcc
    20041 cacatttcgg cgtggcccat catcaccacg tcgagcacgc gcacgtcttc gtaagcgaac
    20101 tggtcctggc gcaggaaggc catgcgctcg ccggaatcga tggcgacgtt gccgccgctg
    20161 ggttccagca cgccggcgag gatcttcatg aaggtggatt tgcccgcccc gttggcgccg
    20221 atgaggccgt agcggttgcc gtccccgaac ttgatggaga cgttctcgaa caggggcttg
    20281 gccccgaact gcatggtgat gttgttggcg atgagcacga cgggaattcc tcaggcgccg
    20341 tccggcggga cggcaaaacg aaaacgccgg cgcgcggccg gcgaagctgg tacaaatttt
    20401 accgatgatt caagcacctg agcaaacttc ccggtgcctg tcgaaggcgt tgctcagatc
    20461 agcgacggtc cgcgcacgat ctcggccgct tcctgttcgg aaatgcgcac ggtggcggtg
    20521 gccatgacgt gggtggtgag ccattcgtgc agacgccgtt caccatcgtc gtcgaaggac
    20581 aggaagcggc agccgagctg gccgccgcct tcgcccagca catggttcat ggtctcggcc
    20641 tggccccgaa tcgtgtccgg aatgccttcc agatgcaggc tgaaggtaaa gcggcgcggc
    20701 tgctcgggcg gcgtgtcgag cggatagctg aaaccttcga gggacagatc attgagcgcc
    20761 tgccgctcgc cgccgtcgat ggagacccag aagcacaccg cgccccagcg gcgcgcgacg
    20821 aagcgctggc gggtgcggcg ttcgctgtaa tcggggtcct ggctcatcga gagcatcctc
    20881 ctgcggggta gagacgggta cgtcgcaatg ctaggacaaa tccgcggatt cgcccatggg
    20941 gccgccggtg acgtcttgct tcagggaagc atgccacgga aatcatggcg aatgccgttt
    21001 gcactgtcgg aaatttttac tgtttccatg tgaaacacat tttatagatc ataaatatat
    21061 gatttaaatc ataaaacaca acttgggcac acccgttgct gagcaccggc aggcactgat
    21121 ctgcctgtga catatttaac actcaaacgg gagtttccat gaaaaagcaa tttctcgctg
    21181 ctgcagttct cgcggctgcg tccttcggcg ccagcgccgc gaccgtgtcc ttctccgact
    21241 ccttcggcct ggccacgacc gactggaccc acgccctgac gctccagcag ttcgacacct
    21301 cgctgggtac cctgaactcc gtgaccttct cctacggcgg cgaggtgtcc acctccttcc
    21361 gcctggaaag cctggatgcc gccgcggcca acgtgaccgc caacgccgcc ggcaacctgg
    21421 tgttcggtgg cccgatctcc aacaccctcg ccgtgtccgg ctccgccagc cgctccctga
    21481 gcgcttttga cggcgccatc gacttcggcg gcacgtccgg cgccatcgtt ggtcccgtgg
    21541 tcggcaacaa gtccgacgtg ctgaccctgc tgtccggcct gtccgtgtac gaaggtctgg
    21601 gtacctacac catcaacgtg gacgcgcgtg gcctgtccaa cgcctccggt gccggcaacc
    21661 tggtgtccat catcaacacc caggctctgg ccaacatcac cgtgacctac gactacaccg
    21721 tcacgcctcc gggccgtgtg ccggaacccg cctccatggc gctggtgggc ctcggcctgg
    21781 ccggtctggg cgcgatccgt cgccgcaagt aagcacgacg ttgtacaggg gccgttccaa
    21841 cgtgacggga acggcccctg gaatgaaaaa ggccaccgtg aggtggcctt tttcatgagc
    21901 agacggcgct tttacttggc cgcagcgtca gcctcgcact tcttgacgaa gctggacttg
    21961 gcggcgccat ggagcttctt ctccttggcc tggccttcac aggcggacgg agccgtggcg
    22021 ccaccccctt cacatttctt gatgaaactg ttcttggcgg cgccagcgag cttcttggcg
    22081 gcagcctgct cggcacaggt ggcgtcggca gccagggcgc tggaggcgag accggcagcg
    22141 aaggtggaca cggcaatgct caggatcagc ttcttcatct tctcactcct aggtgtgggc
    22201 gggactgctc cactataaac gggccgttgt gcacgatgtt gacccgacgc acgtgcggat
    22261 tcgtgaaact ctgtgacaac gatcaataac cgtcgtcttc catggcgcgg cgctggcgct
    22321 cgacgaactc ctgggtggaa ttgatgccac gcaggagcag gatcgtggaa cgcactgccg
    22381 cctccaggag cactgccagg ttacggcccg cggccaccgg cagcaccacg cggcggatcg
    22441 gcacgcccag gatgttctgt tcctcgccct ccagcgtcag gcgcagcggc tcatcctggc
    22501 tggcctgacg acgctgaagg tgtacgacca gcttgagctt catcttcttg cggcaggccg
    22561 tctcgccaaa gatggtgcgt acattgagaa tgcccaggcc ccgcacttcg atgacgtcct
    22621 gaagcagctc ggggcaacgg cccaccagca ggttgggcgc gatgcgggaa aattcgacga
    22681 tgtcgtcagc caccaggccg tggccgcgag agatgagttc gagcgccagt tccgatttgc
    22741 cggccccgga gtcgccggtg atgaacacgc cgacgcccag cacgtccatc aacacgccat
    22801 gcagggcgat ccgctcggcg agctgacgtg acagatagcc gcgcagctgg tccacgatgc
    22861 tcgccgatgg cagcggggtg gcaaacaggg ccacgtcggc attggcgcac agcgtgcgga
    22921 tcatgctcgg gatgtcctcg tcgtcggcga cgatcagcgc cggcggccgg tacgagagaa
    22981 tgtccttgac gtggtggacg accttttcgc gcttctggcg gcgcgcccac atgagctcgg
    23041 aagaccccag gatctgcagg cgttcggggt ggatcaggtt gaggtggccg accatgtcgg
    23101 ccggccagat gcggtcctcg ggcacgctga tgcgggtttc caggctgcca cagatatggc
    23161 gcagccggag gcgctccagg ttgtcgtcaa acagccgggc gaccgtggtc tgccgcatcg
    23221 ccgccccact gggcaaacag ttcatggacg gccgcagcat ccggtgccgt ggcaagctgg
    23281 tggcggaagg cccggtcgct gaacatctgg gccagttccg aaaggagctg aagatgatgt
    23341 tcggtggctt gctccggcac cagcaggacg aacaggagat tcaccgggcg cccgtccggc
    23401 gcatcgaact gcaccggctc ggacaggcgc atgaaggcgc cgagggcgtc cttgagaccc
    23461 ttgatgcgtc catggggaat ggcaatgcct tgcccgagcc cggtcgaccc cagtttttcg
    23521 cgggcgaaaa ggctgtcgaa caccatactg cgcccgatgc cgtgattgtt ctcgaagagc
    23581 aggccggcct gctcgaacgc gcgcttcttg ctgctagcct cgaggccgac gaccacgttg
    23641 gcgggtggca gaagttgggc gatgaggttc atgggcggga aggaccagac cggaacgtgc
    23701 ttccgatcgc gatcgggatc ggggtgggag aaacccggcg gggggccgga ccgtcgtgtc
    23761 tattgggggc gcattatagc cacaacggtc tggctgaaaa gtgcctgaaa cttcaggcgt
    23821 tcaggattca gcgacgtgat gcttgaggga gtcgtgaccg tggtcgctgg tcttggcctt
    23881 gtacttcaga acctggcggt cgagcttgtc ggccagcgca tcgatggcgg catacatgtc
    23941 ggcatcgtcg ctctcgacgt ggatgtcctt gccgcgcaca tgcacggtga cttcggcctt
    24001 ctgacgcagc ttttccaccg agaggatcac gccgacgctg gtgacgttgt cgaaatgacg
    24061 gatcac
//
LOCUS       DAOQMM010000050        23652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_699_length_23652_cov_53.684663, whole genome shotgun sequence.
ACCESSION   DAOQMM010000050 DAOQMM010000000
VERSION     DAOQMM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23652
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_23652_cov_53.684663"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLH85_09775"
     CDS             <1..543
                     /locus_tag="PLH85_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNB64894.1"
                     /translation="PRGMPQIEVTFDIDANGILHVSAKDKATGKENKITIKANSGLSD
                     DEIQRMVKDAEAHAEEDKKAHELVDARNQLDTLVHSVKKALTEHGDKIDGDEKGKIEA
                     AIKEAEDAIKSGDKAAIDAKSEALATVSQKLGEKVYAQAQAEAAAGGAGAGAGAGSSG
                     AKASDADVVDAEFTEVKDKK"
     gene            638..1765
                     /gene="dnaJ"
                     /locus_tag="PLH85_09780"
     CDS             638..1765
                     /gene="dnaJ"
                     /locus_tag="PLH85_09780"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNB64895.1"
                     /translation="MAKRDLYEVLGVNRDASDDELKKAYRKLAMKYHPDRNPDNKDAE
                     EKFKEAKEAYEILTDANKRAAYDRYGHAGVDPSMGGGGGGGQGFEGFADAFGDIFGDI
                     FGGGGGRGRSNVYRGADLRYNLEISLEEAARGADKTIRIPAVEECDSCHGSGAKPGTQ
                     PKTCPTCGGAGQVRIQQGFFSIQQTCPNCHGSGRIIPDPCKACGGAGRVKRQKTLEVK
                     IPAGIDSGMRLRHGGHGEPGVNGGPAGDLYVEIHIKAHPVFQRDGDDLHCEMPISFAT
                     AALGGDIEIPTLDGAASIRIPPETQSAKVFRLRGKGIRNVRSHAPGDLMVHVIVETPV
                     KLTDRQKELLREFDDIASRNSERHNPKATSWMDKMKNFFSG"
     gene            1927..3084
                     /locus_tag="PLH85_09785"
     CDS             1927..3084
                     /locus_tag="PLH85_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB64896.1"
                     /translation="MKLIHAIKSAAASLGALAVLSLSTPLCNADPGVTDSTITLGMSA
                     PFSGPNGAYGAEMKEGALAYFAQLNAAGGIGGRKVDLVALDDGYDTERAVSNTRKLIN
                     ESNAFALMAFYGSSPTTESMKVFAEAKVPLIGTISGASTLRTPVNRYMFHLRASYADE
                     TETLVNHLVGLGITHIAVFYQNDGFGKSGLEGVTAALGRHKLKPVAIGSVERNSIQVE
                     NAVKDIAKVSPQAVIMVTLYKPTAEFIRQMRKAGQMPQFSTLSPIGADLIVNELGAQQ
                     AHGLGISQVMPYPWNDTVPVVRDYQRALQAFAKHKNYSYYGLEGYLNAKLVAEALRRA
                     GKEPTREKLIAALEGGPFELGGYKVAYSSSNHNGSRFVDLTVLSRDGRVLR"
     gene            complement(3160..4542)
                     /gene="cysS"
                     /locus_tag="PLH85_09790"
     CDS             complement(3160..4542)
                     /gene="cysS"
                     /locus_tag="PLH85_09790"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNB64897.1"
                     /translation="MLKIHNSLSRKKEQFCPIEPGKVRMYVCGMTVYDFCHLGHARVM
                     VVFDMVARWLRASGYAVTYVRNITDIDDKIIKRAQENGESIRSLTDRFIDAMHEDADA
                     LGVLRPDHEPRATEYVPAMQTLIGSLVEHGLAYKAANEDMCYAVRKFDGYGKLSGKSL
                     DELRAGERVEVAGGKQDPLDFVLWKHARADEPEEVKWASPWGAGRPGWHIECSAMSSE
                     LLGAQFDIHGGGADLQFPHHENEIAQSEGAHQCTFVNYWMHNGFVRVDDEKMSKSLGN
                     FFTIRDVLKAYAPEVVRFFILRAHYRSPLNYSDAHLVDARHALSRLYTALRNVPADGT
                     VVNWDHPLAVRFKAALDDDFNTAEAIAVLFDLANEVNRERAPAMAALFKGLANILGLL
                     EQDPAAFLQAGTEAGGEDEGAAIQARIEARLAAKKARNFAEADRIRAELLADGIVLED
                     TPQGTIWRRA"
     gene            4785..5915
                     /locus_tag="PLH85_09795"
     CDS             4785..5915
                     /locus_tag="PLH85_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB64898.1"
                     /translation="MSKRALAASLLLACLAASTPVLADAPQDIQALIKQGQFTQALER
                     TDQALAGKPRDAQLRFLKGVVLTELNRGNDAIAIFQKLTEDYPELPEPYNNLAVLYAQ
                     QRQYDKARNALEMAIRTHPSYATAHENLGDVYARLASQAYDKALALDSSNTSAQNKLA
                     LIREMISPTGKNVRPTAPAPVSVAAAPAAPAVTPPPVAKPAPAPVPATPAAPAPTTVA
                     QAAPKAPPVVASPPVPPAPPPAAKPTQKDDPKPAAKPEPRDESAAQSAAITRSVQGWA
                     TAWSHKDIKGYLSYYARDFDPPKGLSRKAWEEERGQRLTKPGSIEVALENVQIASVAG
                     DKATVRLRQHYKSANLSTSSNKTLILVRQDGKWLIQQERVGG"
     gene            6020..7132
                     /locus_tag="PLH85_09800"
     CDS             6020..7132
                     /locus_tag="PLH85_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNB64899.1"
                     /translation="MVAAPGGPDAALAEALQDVSQGRLTTALARIDRLIAAYPTFRLA
                     QLIRGDLLLAQAGPISGFGNVAEAPPERLADLRDEAVVRLKAFRNRPPANYVPRYLLQ
                     MEPEQKYAVVVDTQSARLYLYQNDAGRPRYVADYYISHGKLGAEKFREGDKRTPIGVY
                     YVTSSLPRQKLNDFYGSGAFPISYPNEWDRQQGRDGHGIWLHGTPSDTYSRPPRASDG
                     CVVLTNPDLEALGGYLQIGITPVIISHNIEWLSLEDWAGERTALGKAVEAWRSDWESR
                     DVKRYLSHYSSRFRNNEGGYDEWSRQKQLVAAGKSWIKVSLNHLSIFRNPGKQDMAVV
                     SFEQDYRSNNLSNTLRKRQYWMKEGGRWKIVYEGFA"
     gene            7171..7740
                     /locus_tag="PLH85_09805"
     CDS             7171..7740
                     /locus_tag="PLH85_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB64900.1"
                     /translation="MKCALAALILASQALVASAAPQVELKTTAGTIVVDLNSEKAPKT
                     VANFVDYVKAGHYNGVIFHRVIDGFMIQGGGFTPEMKEKPTRAPIQSESKNGLKNDAG
                     TLAMARTQDPHSASAQFFINLVDNAPLNYPSPDGWGYTVFGKVVTGMDVVRKIGQTPT
                     GNRGFYRDVPLEAVVIQSARVLPDAPAAK"
     gene            7773..8267
                     /locus_tag="PLH85_09810"
     CDS             7773..8267
                     /locus_tag="PLH85_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB64901.1"
                     /translation="MAVKLVTNFGDITLELDADKAPVTVKNFLGYVEAGHYNNTVFHR
                     VINGFMIQGGGFEPGMKQKDTGEQIQNEANNGLKNDAGTIAMARTQAPHSATAQFFIN
                     VADNDFLNHRSPDIQGWGYCVFGRVTEGMDVVNKIKGVKTGSKGFHQDVPVEDVIIER
                     AEIL"
     gene            8296..9021
                     /locus_tag="PLH85_09815"
     CDS             8296..9021
                     /locus_tag="PLH85_09815"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNB64902.1"
                     /translation="MRVHFISDLHLSADRPALIGLFERYLAGPARTAEQLYILGDLLE
                     YWCGDDDTDDPLARRLCGALKDLSASGCQVSFMAGNRDLLIGPAFAERAGFTLLPEPT
                     LIELDGQPTLLCHGDSLCTDDVAYQAYRTQVRDPVWQQGFLAQPLEARKHFVQGLRMK
                     SEAAKSGKAADIMDVNADAVAGLLRAHAYPRLIHGHTHRPATHHLTVDGHACERWVLA
                     DWHDDQGEVLIWDGDRLLRQILR"
     gene            complement(9091..10263)
                     /locus_tag="PLH85_09820"
     CDS             complement(9091..10263)
                     /locus_tag="PLH85_09820"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNB64903.1"
                     /translation="MNPLHPETLAVRAGTARSQFNEHSEALYLTSSFVFDSAAQAAAR
                     FSGEEEGNVYARFTNPTVTAMQQRLAALEGAESCVATASGMAAILATVMALMQAGDHV
                     VASRGLFGATQQLFGNILSKFGIETSFVPATDLAAYRAAVTPRTKLFFVESPSNPLTE
                     LVDIGAVAAIAHEAGALLAVDNCFCSPALQQPLALGADLVIHSATKYLDGQGRVLGGA
                     VCGAAQHVDEVFKFLRTAGPCISPFNAWVILKGLETLSIRMAAQSAAALELARWLEAQ
                     PQVARVHYPGLPSHPQHELALRQQKSGGAIVSFEVQGGRDEAWRVVDHTQVMSITANL
                     GDTKTTITHPATTTHGRISPEAREAAGIRESLLRVAVGLESVEDLKADLARGLALL"
     gene            complement(10270..11793)
                     /gene="purF"
                     /locus_tag="PLH85_09825"
     CDS             complement(10270..11793)
                     /gene="purF"
                     /locus_tag="PLH85_09825"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNB64904.1"
                     /translation="MCGILGVVATTRVNQLLYDGLQVLQHRGQDAAGIATAEGGRFHM
                     HKSSGLVRDVFRTRNMRDLPGNWGIAHVRYPTAGSASSAAEAQPFYVNSPFGIMLAHN
                     GNLTNAEDLKREMFLSDLRHINTNSDSEVLLNVLAHELQAAASSFRLDQDTVFKAVAG
                     VHRRCRGAYATVAMISGTGLLAFRDPYGIRPLVIGRNDTPAGTEWMVASESVALDVLG
                     FRLVRDVAPGEAILIDAEGGFHSRQCATHTVHAPCMFEFVYLARPDSLIDNISVYDAR
                     IKMGEFLARKLRSTMPHLEIDVVIPIPDSSRPSAMEMAHHLNVPYREGFVKNRYIGRT
                     FIMPGLSIRRKSVRQKLNAIHQEFRGKKVLLVDDSIVRGTTSREIVQMARDAGATQVY
                     MASAAPPVRFANVYGIDMPTRHELIASNRTEDEICAEIGADALIYQDLEDLKAAVRAL
                     NPVISVFETSCFDGCYVTGDVTAEYLDGVETQRSEAPSDASEGAAQMDLNLVTGHEG"
     gene            complement(11805..12296)
                     /locus_tag="PLH85_09830"
     CDS             complement(11805..12296)
                     /locus_tag="PLH85_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNB64905.1"
                     /translation="MNGFDYAMLGLLGLSALLGFWRGLISEVVALGAWVLAFLVAKAF
                     LPDVTPWLAAWVREPVLQAPVAFVVIFVGVLVGVALLRWLLRELIQAAGLGLADRFLG
                     ACFGLVRGGVIVFAVAVLVGLAGLAREAWWREASLSAPLETAVLATRPWLPDPLTKRL
                     RFR"
     gene            complement(12310..13101)
                     /locus_tag="PLH85_09835"
     CDS             complement(12310..13101)
                     /locus_tag="PLH85_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB64906.1"
                     /translation="MADSDSQLDLKKRARRRLVGSAALALTAAIVLPMVMDHEPRPPS
                     QDVQIRIPSQEGSNFTSRVISGKGPAPTQPVAGAADAAVDDGVALPRDRTVPKTVQPE
                     AGAPAGRVDPVRPQAAVEPPPDVKPEVRPVPKPDAKPEPRKPEPKPEPKKPEPKPEPK
                     PEPEPKPKPPTDAERAKALLNGEAPTAGANGPHVVLLGAYREAANVTALRARLKAEGF
                     ATYTEPLGDKIRVRAGPFPNKAAADKAAARISKMMGVSASVAVKP"
     gene            complement(13088..14386)
                     /gene="folC"
                     /locus_tag="PLH85_09840"
     CDS             complement(13088..14386)
                     /gene="folC"
                     /locus_tag="PLH85_09840"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNB64907.1"
                     /translation="MQWPETLDGWLSLLEVRHDQTIQLGLERVRRVRDALGHPQTCPV
                     FIVGGTNGKGSTCALLEAVLLAAGYRVGLYTSPHLLRYNERVRIDGREASDAELVDAF
                     VEVERARGDVPLTYFEHGTLAAWAAFARAGVEAVILEVGLGGRLDAVNVFEPDCAIVT
                     SVALDHMDYLGSTREAIGFEKAGIFRAGKPAICADPLPPQSLLDHAAAIGAPLWVSGR
                     DFGFAGDQNQWAFWLAQGSRRGGLAYPALRGTNQLLNAAAVMAACEAVKPILPVPMQA
                     IRQGFMLVELPGRFQVLPGRPAVVLDVAHNPQAAGVLNENLASMGFFPETWAVFGMLA
                     DKDVAGVVSLLKDRVDHWRVATLPGPRGLSADGLIALLHDEGVKGDVAGFETPAAAYR
                     AVREQAAEGDRIVVFGSFLTVADVLAGMKVSHQVQQDGGL"
     gene            complement(14439..15311)
                     /gene="accD"
                     /locus_tag="PLH85_09845"
     CDS             complement(14439..15311)
                     /gene="accD"
                     /locus_tag="PLH85_09845"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNB64908.1"
                     /translation="MSWLTKLLPPKIKRDTSSRKAIPEGLWSKCPACEAVLYRSDLES
                     NQNVCPKCGHHQRIRARQRIDLLLDPEGRFEIGAEVVPVDPLKFRDSKRYPERLKAAN
                     EDTGEADALVVVQGAIKTVPVVVACFEFEFLGGSMGSVVGERFVRGVRAALEQRLPFI
                     CATATGGARMQEGLFSLMQMAKTTAAITRLGDRRLPFITLLTDPTMGGVSASFAFMGD
                     VVIAEPGALIGFAGPRVIEQTVRETLPEGFQRSEFLQEKGAVDLIIDRRELRDRLSEL
                     LTLLTRQASVKNAS"
     gene            complement(15308..16120)
                     /gene="trpA"
                     /locus_tag="PLH85_09850"
     CDS             complement(15308..16120)
                     /gene="trpA"
                     /locus_tag="PLH85_09850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNB64909.1"
                     /translation="MSRIQNCFQRLAADGRKALIPFITAGDPAPEMTVPLLQALVAGG
                     ADIIELGVPFSDPMADGPTIQRASERALAAGMSLRKVLDIVRAFRATGDQTPVVLMGY
                     ANPIEAMGVDTFARAAVEAGADGVLVVDYPPEECREFAATCRAAGLDPIFLLAPTSTE
                     QRFADVAAAGSGYIYYVSLKGVTGSAALDLDEVARRIPAIRERVGMPVGVGFGIRDAA
                     TAARIAGLADAVVIGSRIIEEIENSPRDAAPARVTAFLRGVREAMDQRGEAR"
     gene            complement(16174..17385)
                     /gene="trpB"
                     /locus_tag="PLH85_09855"
     CDS             complement(16174..17385)
                     /gene="trpB"
                     /locus_tag="PLH85_09855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNB64910.1"
                     /translation="MHMADAPYDLPDARGHFGPYGGVFVSETLIPALAELREAYAAAQ
                     QDPEFIAEFEYELKHYVGRPSPIYHAKRWSGLLGGAQIYLKREDLNHTGAHKVNNCIG
                     QALLARRMGKPRVIAETGAGQHGVATATVAARYGMECVVYMGSEDVRRQAANVYRMKL
                     LGATVVPVESGSKTLKDALNEAMRDWVTNVGNTFYIIGTVAGPHPYPMMVRDFQAVIG
                     QECIEQMPPLAGRQPDAVIACVGGGSNAMGIFHPYIGFPDVRLVGVEAAGHGLDSGQH
                     AASLSAGRPGVLHGNRTYLLQNEDGQIIETHSISAGLDYPGVGPEHAWLKDSSRAEYV
                     GITDAEALQAFHDLCRFEGIIPALESSHALAYAAKLAPTLPKDKILLVNLSGRGDKDM
                     HTVAEKSGIRF"
     gene            complement(17372..17995)
                     /locus_tag="PLH85_09860"
     CDS             complement(17372..17995)
                     /locus_tag="PLH85_09860"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNB64911.1"
                     /translation="MHRTRIKICGLTREEDVDAAVGAGADAIGFVFYPPSPRHVSFAR
                     AAELARRVPPFVTVVGLFVNTPRAEVDEALRQVPIQLLQFHGDEEEADCAGFGRPYVK
                     AARMRPGLDLLKYAARFPGAQGLLLDAFVEAYGGGGETFDWSLIPSGLPLPLILSGGL
                     HAGNVGEAIRRVRPWAVDVSSGVEAAKGIKDAARITEFVAGVRHAHG"
     gene            complement(18000..18803)
                     /gene="truA"
                     /locus_tag="PLH85_09865"
     CDS             complement(18000..18803)
                     /gene="truA"
                     /locus_tag="PLH85_09865"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNB64912.1"
                     /translation="MPPIRIALGVEYAGDAFEGWQTQPHGRTVQDVLEAAVCVIAQAP
                     IHLVCAGRTDTGVHATTQVAHFDTAAVRPPEAWVRGVNSHLPRGVSVLWSQTVAADFH
                     ARFSAVSRHYRYLLLNRRVRPALLAGRVGWLHDGLDVEAMTAAADCLVGEHDFTSFRA
                     AECQAKSPVRTVYRVAVGRVGDLVVFDFHANAFLHHMIRNLVGALVMVGRGRKPVAWM
                     ADVLAARNRSVAAPTFAPDGLYLCGVEYPPRWPLPGGGRIIVPPQLPLG"
     gene            complement(18805..19404)
                     /locus_tag="PLH85_09870"
     CDS             complement(18805..19404)
                     /locus_tag="PLH85_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HNB64913.1"
                     /translation="MLHPATRLLLWSAWVVVVQIARWPFVGVIGLFVLVAGFWAARTR
                     CARLLRRVRILLGVLVILFAFFTPGEALLPGLGAAGPTREGLAAAGLHGLRLVSVVVL
                     VALLLEKTGQAALVGGLTSLARPLHVLGLSADRLAVRMLLVLQYASAPPVRGWRAFLV
                     EDERPAEPGVIVLPKVRPGWPDVFVWSVLAIGVAAWMFA"
     gene            complement(19431..22391)
                     /locus_tag="PLH85_09875"
     CDS             complement(19431..22391)
                     /locus_tag="PLH85_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNB64914.1"
                     /translation="MHKKTLNNRLRLSLMALAIGTVSLGSGAAGLGRITVMSSLGQPL
                     RAEVEIAATPEELQNITARLASADAFRQAGIDYSPSVTGLRMAVERRGNGAVVRVSSD
                     RPINEPFVDMLVELNWSAGRLIREYTFLLDPPDAGAPRAAAVAVPEVRSDMARPGVAP
                     APAAVRRAPASRPAAVPAASDRSYLVKPGDTLNRIAMETRPDGVSVDQMLSALFQGNR
                     GAFAADDINRLQAGKVLRIPDADGVRQLAPGGRQVVAQSSDFGRYRQRLAEQAAASAP
                     KEAPARQSASGRIGARVEDKAQGLPAGSDQLKVSKGADAASLAGGGADKPSSRLQALE
                     EDLVSRDKALKDANARLAELEKSIRELQRLVELKSQGLAQTQDQAAKPAAAPAVVPPP
                     PPAPVPPPAAVVPPPEAAIKPPEAEKPADLAPPPPPPPAPEVKPAPPPPPAPAPASAE
                     ASSGPDMSLLLGGGGIAALLLGWGAYRLFGKRRNNGAPTTTPAYSEFGEGSSSVFGSA
                     GGQSVDTGSSSQIQTDFSQSGLSSIDTDEGVDPVAEADVYMAYGRDAQAEEILLDALK
                     TDPGRTGVHLKLLEIYAQRKNLKQFETTATELYSLTGGNGPDWEKAAALGRKVDPDSP
                     LYRIPVPGAMPGPSAIPVPAVSELGPHTIAGTMAPATGFVQAGEARQAPPMSARPAPA
                     PAPIAPSGDDDPLESPSQLKDTWALPGELNQYTSLNDLDHHAPSAPEPVAPSRPASLD
                     FDLNLEASSPSGNLATIVTGDGNGPASAGGLDFDLGFEQSVTRGAGATMVVGDEFGRT
                     MEVTSTRPQRVGEDFGLPDPSDLDFEISSEFGERNANPPVAPSVDVDLTATLADEGIR
                     PPNDSATIDLERTNFDGNLLDFDFDLDDRSKADVHPGQATQTDMPAVDLSGIDLDLDR
                     PTVEVDLPDAGPPTEPPLDMDLHQEVATKLELARAYEEMGDKDGARELVEEVLREGNG
                     RQKDEAQAILARLG"
     gene            complement(22528..>23652)
                     /gene="asd"
                     /locus_tag="PLH85_09880"
     CDS             complement(22528..>23652)
                     /gene="asd"
                     /locus_tag="PLH85_09880"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNB64915.1"
                     /translation="MKRVGLVGWRGMVGSVLMQRMREENDFALIEPVFFTTSNPGGKA
                     PNFGKDAPALKDARSIDELKAMDIVITCQGGDYTNEVYPQLRAAGWNGHWIDAASALR
                     MDPKAVIILDPVNLNVIQDSLAKGGKDWIGGNCTVSLMLMALGGLFQRDLVEWATSMT
                     YQAASGAGAQNMRELLTQMGELNRSVKELLDDPASAILDIDREVAGTMRDDAFPSSNF
                     GVPLAGSLIPWIDKDLGNGQSKEEWKGGVETNKILGRGEGFGSPAVPIDGLCVRIGAM
                     RCHSQALTLKLKKDIPLDEIEGLLASANDWVKVVPNQREASMRDLTPTAVTGTLTVPV
                     GRLRKLAMGGEYLSAFTVGDQLLWGAAEPLRRMLRVLLEA"
BASE COUNT         4335 a         8175 c         7407 g         3735 t
ORIGIN      
        1 ccgcgcggca tgccgcagat cgaggtcacc ttcgacatcg acgccaacgg catcctgcac
       61 gtgtccgcca aggacaaggc caccggcaag gaaaacaaga tcaccatcaa ggccaactcg
      121 ggcctgtcgg atgacgagat ccagcgcatg gtaaaggacg ccgaggccca cgccgaagag
      181 gacaagaagg cccacgaact ggtggatgcg cgtaaccagc tcgacaccct ggtgcactcc
      241 gtcaagaagg ccctgaccga gcacggcgac aagatcgacg gcgacgagaa gggcaagatc
      301 gaagccgcca tcaaggaggc cgaggacgcc atcaagtccg gcgacaaggc cgctatcgac
      361 gccaagagcg aagccctcgc gactgtgagc cagaagcttg gtgaaaaggt ttatgcccag
      421 gcccaggccg aagccgccgc cggtggcgca ggggccggcg ccggagccgg cagcagcggc
      481 gccaaggcga gtgacgccga tgtggtggat gctgaattca ccgaagtgaa agacaagaag
      541 taatcgcccc cgggtctccg gacccccacg gccgagcccg aaccggagca gtccgggccg
      601 gctcggcctt tccacacgat agccagagca ggcaaccatg gcgaaacggg atttatacga
      661 agtcctcggc gtcaatcgcg acgcatcgga cgacgaactg aagaaggcct accgcaagct
      721 ggccatgaaa taccatccgg accgcaatcc ggacaacaag gacgccgaag agaaattcaa
      781 ggaggccaag gaggcctacg agatcctcac cgacgccaac aagcgcgccg cttacgaccg
      841 ctacggccat gccggcgtcg atcccagcat gggcggcggc gggggtggcg gtcagggctt
      901 cgagggcttc gccgatgcct tcggcgacat cttcggcgac atctttggcg gcggcggcgg
      961 acggggacgt tccaacgtct accgcggtgc cgacctgcgc tacaacctgg agatctctct
     1021 ggaggaagcc gcccgcggcg ccgacaagac catccgcatc ccggccgtcg aagaatgcga
     1081 cagctgtcac ggcagcggcg ccaaacccgg cacccagccc aagacctgcc ccacctgcgg
     1141 cggcgccggt caggtgcgca tccagcaggg cttcttctcc atccagcaga cctgccccaa
     1201 ctgccacggc agcggccgga tcattcccga tccgtgcaag gcctgcggtg gcgcgggtcg
     1261 cgtcaagcgc cagaagaccc tggaggtgaa gatcccggcc ggcatcgaca gtggcatgcg
     1321 cctgcgccac ggcggacacg gcgagcccgg cgtcaatggt ggcccggcgg gcgaccttta
     1381 cgtggagatc cacatcaagg cacacccggt cttccagcgc gacggcgacg acctgcactg
     1441 cgagatgccc atcagctttg ccacggcggc cctcggcggc gacatcgaga ttcccaccct
     1501 cgacggcgcg gcgagcatcc gcattccgcc ggaaacccag agcgccaagg tcttccgcct
     1561 gcgcggcaag ggcatccgca atgtgcgctc ccacgcccct ggcgacctga tggtgcatgt
     1621 catcgtggaa acgccggtca aactgaccga ccgacagaag gaactgctgc gggaattcga
     1681 cgacatcgcc tcgcgcaatt ccgagcgcca caaccctaaa gccacgtcct ggatggacaa
     1741 gatgaagaac ttcttcagcg gctgatcgac gtccccgtgc cgctcatcga cacaggccgg
     1801 accctcgcgg gtgccggcct gtgtctttgt gccgatcgaa ccgggggcac cgatgtgtct
     1861 tttccgtaac actttcgttt tggatatact cgtcgtcgcc ctcccataac gacaacaggc
     1921 acatccatga agctgatcca cgccatcaag tcagccgccg cgtccctggg cgccctcgcc
     1981 gtcctgagtc tttccacacc gctctgcaac gccgaccccg gggttaccga cagcaccatc
     2041 accctcggca tgtccgcccc cttttcaggc ccgaacggtg cctacggggc cgagatgaag
     2101 gaaggcgcgc tggcctactt cgcccaactc aatgcagccg gtggcatcgg cgggcgcaag
     2161 gttgaccttg tcgccctgga cgacggctac gacaccgaac gcgcggtgtc caacacccgc
     2221 aagctcatca acgaaagcaa cgccttcgcg ctgatggcct tctacggatc ctcgcccacc
     2281 accgagtcga tgaaggtgtt tgccgaggcc aaagtgcccc tcatcggcac catcagtggc
     2341 gcctccacgc tgcgcacgcc ggtcaaccgc tacatgttcc acctgcgcgc cagctatgcc
     2401 gacgaaaccg agaccctggt caaccatctg gtcggactgg gcatcaccca cattgccgtg
     2461 ttttaccaga acgacggatt cggcaagtcc gggctcgaag gcgtcaccgc agcactcgga
     2521 cgccacaagc tcaagccggt cgccatcggc agcgttgagc ggaattcaat ccaggtggaa
     2581 aacgcggtca aggacattgc aaaggtctcc ccgcaggccg taatcatggt caccctgtac
     2641 aagccgactg ccgaattcat ccgtcagatg cgcaaggcgg gccagatgcc ccagttctcc
     2701 accctgtcgc ccatcggcgc cgacctcatc gtgaatgaac tgggcgccca gcaggcccac
     2761 ggtctgggca tctcccaggt catgccctac ccatggaacg acaccgtgcc cgtggtgagg
     2821 gattatcaac gggcccttca ggctttcgcc aagcacaaga actacagcta ttacgggctt
     2881 gaaggctacc tcaacgccaa gctcgtggcg gaagccctac gccgcgccgg caaggaaccg
     2941 acccgtgaaa agctgatcgc ggcgcttgaa ggcggcccct tcgaactcgg cggctacaag
     3001 gtggcctatt cgtccagcaa ccacaacggt tcccgcttcg tcgacctgac cgtcctgtcc
     3061 cgcgacggcc gcgtgctgcg ctgaacgccc cgttcggacc ccatctgtga caagggctcc
     3121 ccgcagggag ccctttttaa ttgcgcgacg cggggtacgt caggcccgac gccagatggt
     3181 accctggggc gtatcttcga gcacgatacc gtcagccagc agctccgccc ggatgcggtc
     3241 cgcctctgca aaattgcgcg ccttcttggc ggccagacgg gcctcgatgc gcgcctggat
     3301 ggcggcgccc tcatcttcgc ccccggcctc cgtcccggcc tgcaggaagg cagccggatc
     3361 ctgctccagc agcccgagga tgttcgccag ccccttgaac aaggccgcca tggctggcgc
     3421 gcgctcccgg ttcacctcgt tggccagatc gaacagcacc gcaattgctt cggccgtgtt
     3481 gaaatcgtcg tccagagccg ccttgaagcg caccgccagg ggatggtccc agttcaccac
     3541 cgtcccgtca gccggcacgt tgcgcaatgc cgtatacaga cgcgacagag cgtggcgggc
     3601 atccaccaga tgggcatcgg aatagttcag cggactgcga tagtgcgcac gcaggatgaa
     3661 gaaacgcacg acttccggtg cgtaggcctt gagcacgtca cggatggtga agaagttgcc
     3721 gagactcttc gacattttct cgtcgtccac ccgcacgaac ccattgtgca tccagtaatt
     3781 cacgaaggta cactggtggg cgccctcgct ctgggcaatc tcgttctcat ggtgcggaaa
     3841 ctgcagatcg gccccgccgc catggatgtc gaactgcgcg ccaaggagtt ccgagctcat
     3901 cgcggagcac tcgatgtgcc agcccggtcg gccggcgccc cagggcgagg cccacttgac
     3961 ctcctccggt tcgtccgcac gggcgtgctt ccacaacacg aaatccagcg gatcctgctt
     4021 gccgccggcc acctccaccc gctcaccggc gcgcagttcg tcgagggact tgcccgagag
     4081 cttgccgtag ccgtcgaact tgcgcaccgc gtaacacatg tcttcattgg ccgccttgta
     4141 ggccagcccg tgctcgacca gactgccgat cagcgtctgc atggccggca cgtactccgt
     4201 cgcacgcggt tcgtgatcgg gtcgcaggac acccagagca tccgcatcct catgcatcgc
     4261 atcgatgaag cgatcggtca aggaacggat gctttcgccg ttctcctggg cacgcttgat
     4321 gatcttgtcg tcgatgtcgg tgatgttgcg cacgtaggtc acggcataac cgctggcccg
     4381 cagccagcgc gccaccatgt cgaacaccac catcacgcgg gcatgcccca ggtgacagaa
     4441 gtcgtagacc gtcatcccgc acacatacat ccgcaccttg cccggctcga tggggcagaa
     4501 ctgctccttt tttcgcgaaa gggagttatg aattttcagc atgaaggaaa ggaaaaccgc
     4561 aaatggggga aatctctgga atgccgacac gcaccgtcac cagatcgcat gccgcctcac
     4621 gcgaccggag tcggcgcggg ggtcttctcg actggggacg cacgggggaa tgctcatggc
     4681 tggccggcaa gcttcttggt agaatgcgcg gccatgatgc cggtgcgccg gcgacgcaat
     4741 cttcccagaa ctctccgggc ctgaaggcca tctcagtata ccacatgtcg aaacgtgccc
     4801 tggccgcatc cctcctgctg gcctgccttg ccgccagcac tccggtcctg gcggacgccc
     4861 ctcaggacat ccaggcgctg atcaagcagg gccagttcac ccaggcgctc gaacgaaccg
     4921 accaggcgct cgccggcaag ccccgcgacg cccagttgcg cttcctcaag ggtgtcgtgc
     4981 tcaccgaact gaaccggggc aacgacgcca tcgcgatctt ccagaagctg accgaagact
     5041 acccggagct gccagagccc tacaacaacc tcgcggtgct ctacgcccag cagcgccagt
     5101 acgacaaggc ccgcaatgcc ctggagatgg ccatccgcac ccatcccagc tacgccacgg
     5161 cccacgaaaa tctcggtgac gtgtatgcgc gcctggccag ccaggcttac gacaaggccc
     5221 tggcgctgga ctcctccaac acatccgccc agaacaagct ggccctgatc cgcgagatga
     5281 tcagtccgac cggcaagaac gtccgtccga cggcgcccgc accggtcagc gtggccgcag
     5341 cgcctgcggc ccccgccgtg acgccgcctc cggtcgccaa accggctcct gccccggtac
     5401 cggcgacacc tgccgccccg gcgccgacca ccgtcgccca ggcagccccc aaggcgccgc
     5461 ccgtcgtcgc ctcgccccct gtcccgccgg caccgccccc cgcggccaag ccgacacaga
     5521 aggacgatcc caagcccgct gccaaacctg aaccccgcga cgaaagcgct gcccagtcgg
     5581 ccgccatcac ccgttcggtc cagggctggg cgaccgcctg gtcccacaag gacatcaagg
     5641 gctacctgtc ctattacgcc cgtgacttcg atccacccaa ggggctcagc cgcaaggcct
     5701 gggaagagga acgcggccag cgcctgacca agccgggcag catcgaggtg gccctcgaga
     5761 acgtccagat cgccagcgtc gcgggtgaca aggccaccgt gcggctgcgt cagcattaca
     5821 agtctgccaa cctgtccacc tcgtccaaca agacgctcat cctcgtccgt caggacggca
     5881 agtggctgat ccagcaggag cgggtcggcg gttgatgcgg tcggtgatgg cggtctttcg
     5941 ctgggcgtgc gccgccgtgc tggcgtgcgg cgcacttgcg gccctttcgg gaccgccgcc
     6001 tgcaacggcg cccgcgccga tggtcgcagc gcccgggggg cccgatgccg ccctcgccga
     6061 ggccctccag gatgtcagcc agggacgtct gacaaccgcc ctcgccagga tcgaccggct
     6121 catcgcggcg tatcccacgt tccgtctggc ccagttgatc cgtggcgacc tgctgctggc
     6181 ccaggccggc cccatcagcg gcttcggcaa cgttgccgaa gccccgcccg agcgcctcgc
     6241 cgacctgcgc gacgaagccg tggtgcgcct gaaggccttc cgtaaccgcc cgccggccaa
     6301 ctacgtgccc cgctacctgc tgcagatgga gccggaacag aaatacgccg tcgtcgtgga
     6361 cacccagagt gcccgtctct atctctatca gaacgatgcc ggccggcccc gttacgtggc
     6421 ggattactac atttcccatg gcaagctggg tgcagagaaa ttccgtgaag gcgacaagcg
     6481 cacgcccatc ggcgtctatt acgtcacctc cagcctgcca cgccagaaac tcaacgactt
     6541 ctacggcagc ggcgccttcc ccatcagcta cccgaatgaa tgggatcgtc agcagggtcg
     6601 ggacggacac ggcatctggt tgcacggcac gcccagcgac acctattcgc gcccgccgcg
     6661 cgcctctgac gggtgtgtcg tgctcaccaa ccccgatctg gaagccctcg ggggctacct
     6721 gcagatcggc atcacgccgg tcatcatcag ccacaacatc gaatggctga gcctcgaaga
     6781 ctgggccggc gaacgcacgg ccctcggcaa ggcggtcgag gcctggcgct cggactggga
     6841 aagccgtgac gtgaagcgct acctgtccca ttattccagc cgcttccgca acaacgaagg
     6901 cggctacgac gaatggtcgc gacagaagca gctggtcgcg gccggcaagt cgtggatcaa
     6961 ggtcagcctc aatcacctga gcatcttccg caaccccggc aagcaggaca tggccgtcgt
     7021 cagcttcgag caggattacc gcagcaacaa cctgtccaac acgctgcgca aacgtcagta
     7081 ctggatgaag gaaggcggtc gctggaagat cgtctacgaa ggcttcgcct gatccaggtt
     7141 cctcttaccc gcactcccgg agatcgcccc atgaaatgcg ccctcgccgc cctcatcctt
     7201 gcttcccagg cccttgtggc cagtgctgca ccccaggttg aactgaagac caccgccggc
     7261 acgatcgtcg tcgacctgaa cagcgagaag gcgcccaaga ccgttgccaa tttcgtcgac
     7321 tatgtgaagg ccggccacta caacggcgtg atctttcatc gggtcatcga cggcttcatg
     7381 atccaaggcg gcggcttcac ccccgagatg aaggaaaaac ccacccgcgc gccgatccag
     7441 agcgaatcca agaacggcct caagaacgac gccggcaccc tcgccatggc ccgcacccag
     7501 gacccccact ccgcgtccgc ccagttcttc atcaacctcg tggacaacgc ccccctcaac
     7561 tacccctcgc ccgatggctg gggctacacc gtattcggca aggtcgtgac cggcatggac
     7621 gtggtccgca agatcggcca gactcccacc ggcaaccggg gcttttaccg ggacgtgccc
     7681 ctcgaagccg tcgtcatcca gagcgcccgc gtgctgcccg acgcccccgc ggccaagtaa
     7741 acaggttttt tcccttccaa caggagcacc ccatggctgt caaactcgtg accaacttcg
     7801 gcgacatcac ccttgaactg gacgccgaca aggcccccgt caccgtcaag aacttcctcg
     7861 gctacgtgga agccggccac tacaacaaca ccgtcttcca ccgcgtcatc aacggcttca
     7921 tgatccaggg cggcggcttc gaacccggca tgaaacagaa ggacaccggc gagcagatcc
     7981 agaacgaagc caacaacggc ctcaagaacg acgctggcac catcgccatg gcgcgcaccc
     8041 aggcacccca ctccgccacc gcccagttct tcatcaatgt ggccgacaac gacttcctca
     8101 accaccgctc ccccgacatt cagggctggg gttactgcgt cttcggccgc gttaccgaag
     8161 gcatggacgt ggtgaacaag atcaagggcg tcaagaccgg ctccaagggc ttccaccagg
     8221 acgtgccggt ggaagacgtc atcatcgagc gcgccgagat cctctgatcc ggcctgtcag
     8281 cggggcttcc ggcacatgcg cgtccatttc atctccgatc tgcacctgag tgccgaccgc
     8341 cccgccctca tcgggctgtt cgaacgctac ctggccggcc cggcacgtac ggcagaacag
     8401 ctctacatcc tcggtgacct gctcgaatac tggtgtggtg acgacgacac ggatgatccg
     8461 ctggcgcgcc gcctgtgcgg tgccctgaag gacctttccg cgtccggctg tcaggtgagc
     8521 ttcatggccg gcaatcggga tctgctcatc ggccccgcct tcgccgagcg tgccggcttc
     8581 acgctgctgc cggagccgac gctgatcgag ttggacggac agccaacact gctgtgtcac
     8641 ggcgacagcc tctgcaccga tgacgtggcc taccaggcct accggaccca ggtgcgggac
     8701 ccggtctggc agcagggctt cctggcacaa ccgcttgaag cccgcaagca tttcgtccag
     8761 ggcctgcgca tgaagagcga ggcggccaag agcggcaagg cggccgacat tatggacgtc
     8821 aatgccgacg cggtggccgg actgctgcgc gcccacgcct atccgcggct catccatggc
     8881 cacactcacc gcccggcaac ccatcacctc acggtggatg ggcacgcctg cgaacgctgg
     8941 gtcctggccg actggcatga tgaccagggc gaagtcctga tctgggacgg cgaccgcctg
     9001 ctgcgccaga tcctgcgctg aaaaaacgaa tgcccggacc ttgccgggca tttccgttta
     9061 ccgcagcgcc ggcacagagg ccggtttgcc ttacaaaagg gccagcccgc gggccagatc
     9121 cgctttcaga tcctcgacgg actccagccc gacggccacc cgaagcaggc tttcacggat
     9181 accggcggcc tcccgcgctt cagggctgat ccgtccgtgg gtcgtggtgg caggatgggt
     9241 gatggtggtc ttggtatccc ccagattggc ggtaatggac atcacctggg tgtggtccac
     9301 cacccgccag gcttcgtcgc ggccgccctg cacctcgaag ctcacgatgg caccgccgct
     9361 cttctgctgc cgcaaggcca gttcatgctg gggatgggac ggcagcccgg ggtaatgcac
     9421 ccgcgccacc tgcggctggg cttcgagcca gcgtgccagt tcgagcgccg ccgctgactg
     9481 ggccgccatg cggatggaca gggtctccag gcccttgagg atcacccagg cattgaaggg
     9541 cgagatgcac ggaccagccg tgcgcaggaa cttgaagacc tcatccacgt gctgtgccgc
     9601 accgcagacc gcaccgccca gcacccgccc ctgcccgtcc agatacttgg tcgcggaatg
     9661 gatgaccaga tcggccccca gggccagcgg ttgctgcagg gccggtgaac agaagcagtt
     9721 atccaccgcc agcagcgccc cggcctcatg ggcgatggcg gccacggcgc cgatgtccac
     9781 cagttcggtg agcggattgg acggggattc gacgaagaac agcttcgtac ggggcgtcac
     9841 cgccgcccgg taggccgcca gatcggtggc cggcacgaag ctcgtctcga taccgaactt
     9901 ggacaggatg ttgccgaaca gctgctgggt cgccccgaac agcccccggg aagccaccac
     9961 atggtcgcct gcctgcatca gcgccatcac cgtcgccagg atcgccgcca tgccagacgc
    10021 ggtggccacg caggattccg caccctccag ggccgccaga cgctgttgca tggcggtgac
    10081 cgtcgggttg gtgaaccggg cgtagacatt gccttcctct tcgccggaaa accgcgccgc
    10141 tgcctgggcg gcactgtcga agacaaagct ggaagtcaga tagagcgctt ccgaatgctc
    10201 attgaactgg ctgcgggccg tgccagcccg gacggccagg gtttcgggat gaagtgggtt
    10261 catgatcctt cagccctcgt gcccggtgac gagattcagg tccatctgcg ctgcgccctc
    10321 ggacgcatcg gacggcgcct cactccgctg cgtctcgacg ccgtcgaggt attcggcggt
    10381 cacatcaccg gtcacgtaac agccgtcgaa gcacgaggtc tcgaacaccg agatgaccgg
    10441 attgagcgca cgcaccgccg ccttcagatc ctcaagatcc tggtagatca acgcgtcagc
    10501 gccaatctcg gcacagatct cgtcctcggt ccgattgctg gcgatgagct catgacgcgt
    10561 cggcatgtcg atgccgtaca cgttggcaaa gcgcaccgga ggcgccgccg acgccatgta
    10621 gacctgcgtt gcgccggcat cgcgggccat ctgcacgatc tcgcggctgg tggtcccgcg
    10681 cacgatggaa tcgtccacca gcagcacctt cttgccgcgg aattcctgat ggatcgcatt
    10741 gagcttctgg cgtacggact tccgacggat ggacaggccg ggcatgatga aggtgcggcc
    10801 gatgtagcga ttcttgacga agccttcccg atacgggacg ttgagatgat gggccatctc
    10861 cattgccgat ggacgactgg aatcggggat cgggatgacc acgtcgattt ccaggtgcgg
    10921 catcgtggaa cgcagcttgc gcgccaggaa ttcacccatc ttgatgcggg cgtcgtacac
    10981 cgagatgttg tcgatcagtg aatcaggccg cgccaggtag acgaactcga acatgcacgg
    11041 cgcgtgcacc gtgtgcgtcg cacactgacg gctgtggaat ccaccctccg catcgatgag
    11101 gatcgcctca ccgggcgcca cgtcccgcac caggcggaaa ccaagcacat ccagggccac
    11161 gctctcggac gccaccatcc actcggtgcc ggcgggcgta tcgttccggc cgatcaccag
    11221 cgggcggatg ccatagggat cgcggaaggc caggaggccc gtaccggaga tcatggccac
    11281 cgtggcatag gctccgcggc agcgccgatg cacgccggcc acggccttga aaaccgtgtc
    11341 ctgatcgaga cggaagctgc tcgcggcggc ctggagttcg tgcgccagca cattgagcag
    11401 cacctccgaa tcggagttgg tgttgatgtg gcgcaggtcg gagagaaaca tctcccgctt
    11461 cagatcttcg gcgttggtca ggttgccgtt gtgggccagc atgatgccga acggcgagtt
    11521 cacgtagaac ggctgcgcct ccgccgcgga cgacgcggaa ccggcggtcg gatagcgcac
    11581 gtgggcgatg ccccagttgc cgggcagatc ccgcatgttg cgcgtacgga acacatcgcg
    11641 caccaggccc gaggacttgt gcatgtgaaa gcgcccccct tcggccgtgg cgatgcccgc
    11701 cgcgtcctgg ccccggtgct ggagcacctg gaggccgtca tacaggagct gattgacgcg
    11761 tgtagtcgcg actacgccca gaatcccaca catttcaaac caccttatct gaatcgaagt
    11821 ctctttgtca gggggtcggg cagccacggc cgggtagcca gcaccgccgt ttccagcggc
    11881 gcggacagac tcgcttcgcg ccaccacgct tcccgggcga ggccagccag tccgaccagc
    11941 accgcaactg caaatacgat cacgccaccc cgcacgaggc cgaaacaggc ccccaggaaa
    12001 cggtccgcca gtcccagccc ggctgcctgg atcaactcgc gcagcagcca gcgtagcagg
    12061 gccacaccca ccagtacgcc cacgaagatc acgacgaagg ccacgggcgc ctgcagcacc
    12121 ggctcacgca cccacgcggc gagccacggc gtcacgtcgg gcaggaaggc ctttgccacc
    12181 agaaaagcca gcacccaggc acccagcgcg acaacttccg agatcagccc gcgccagaac
    12241 ccaagcagcg cggagagccc caggagcccg agcatggcgt agtcgaatcc gttcaagaac
    12301 ctgcgcgacc tagggcttga ccgccactga cgcggacacg cccatcatct tcgagatgcg
    12361 cgcggccgcc ttgtccgccg ccgccttgtt ggggaagggc ccggcccgca cccggatctt
    12421 gtcgcccagc ggctcggtgt aggtcgcgaa tccctcggcc ttgagccgtg cccggagggc
    12481 cgtcacattc gccgcttcgc ggtaggcgcc cagcagcacc acatgggggc cgttagcccc
    12541 ggccgtcggc gcctcgccgt tgagcagggc cttggcccgc tccgcgtcgg tgggaggctt
    12601 gggcttgggt tccggctccg gcttgggttc cggtttaggt tccggcttct tgggctccgg
    12661 cttcggttct ggcttcctgg gctcaggttt cgcgtccggc ttgggcaccg gacggacttc
    12721 aggcttcacg tcgggcggag gctccaccgc cgcctgtggc ctgaccggat ccacacggcc
    12781 ggcaggtgca ccggcctccg gttgcaccgt cttgggcact gtcctgtcgc ggggcagcgc
    12841 gacgccgtca tcgacagccg catcggccgc tccggcaaca ggctgagtcg gcgcaggccc
    12901 cttgcccgag atcacccgcg acgtgaaatt gctgccttcc tggcttggaa tgcggatctg
    12961 cacgtcctgg gagggcggtc gcggttcgtg gtccatgacc atgggcagca cgatggccgc
    13021 cgtcagggcc agcgccgccg atccgaccag gcgccgtcgc gcccgcttct tgagatcgag
    13081 ctgggaatca gagtccgcca tcctgctgca cctgatgcga gaccttcatg ccggcgagaa
    13141 cgtccgcaac ggtcaggaag gatccaaaaa cgacaattct atcaccctcg gctgcctgct
    13201 cacggaccgc ccggtaggcg gcagcgggcg tctcgaagcc ggccacatcc cctttgacgc
    13261 cttcgtcatg gagaagcgcg atgagtccgt ccgccgacag tccgcgtggt ccgggcaagg
    13321 tagccacacg ccagtgatcc acacgatcct tcagcaggct gacgacgccg gccacatcct
    13381 tgtccgccag catcccgaag accgcccagg tttccgggaa gaagcccatg ctcgccaggt
    13441 tttcgttgag cacgccggcc gcctgcgggt tgtgggccac gtccagcacc acggcaggcc
    13501 ggcccggcag cacctggaag cgcccgggca gctccaccag catgaagccc tggcggatcg
    13561 cctgcatggg taccggcaga atgggcttga cggcctcaca ggccgccatc acggcggccg
    13621 cgttgaggag ctgattggtg ccacgcagtg ccggataggc caggccgccc cgacggctgc
    13681 cttgggcgag ccagaaggcc cattgattct ggtcgccggc aaagccgaaa tcccggccgc
    13741 tgacccacag gggcgcgccg atcgccgcgg catggtccag cagggactgg ggcggcagcg
    13801 gatcggcgca gatggccggc ttgccggcgc ggaagatccc ggctttctcg aagccgatcg
    13861 cctcgcgggt gctacccagg taatccatgt gatccagggc cacgctggtg acgatggcgc
    13921 agtccggttc gaagacattg accgcgtcca ggcgtccgcc cagccccact tcgaggatca
    13981 ccgcctctac gccggcccgc gcaaaggcgg cccaggcggc caaggtgccg tgctcgaagt
    14041 aggtcagggg tacatcaccg cgggcgcgct cgacctcgac gaacgcgtcc accagttccg
    14101 catcactggc ttcgcggccg tcgatgcgca cgcgctcgtt gtagcgcagc aggtgcggcg
    14161 aggtgtacag cccgacccgg tagccggcgg ccagcagcac ggcttcgagg agcgcacagg
    14221 tggagccctt gccgttggtg ccgccgacga tgaacacggg gcaggtctgg ggatggccaa
    14281 gcgcgtcgcg cacgcgacgg acgcgctcca ggccaagctg gatggtctgg tcgtgccgca
    14341 cttcgagcag gctcagccag ccgtccaggg tttcaggcca ctgcattcag atctcccgcc
    14401 ggggcgtacc gcacatcggc gccgtggtcg gcgtggtgct aggaggcatt cttgacggac
    14461 gcctggcggg tcagcagggt gagcagttcc gagaggcggt cgcgcagttc gcgacggtcg
    14521 atgatcaggt ccaccgcccc cttttcctgc aggaactcag agcgctggaa gccttcgggc
    14581 agggtctcgc gcaccgtctg ctcgatcacc cgcggaccgg caaaaccgat cagggcgccc
    14641 ggctcggcga tcaccacgtc ccccatgaag gcgaagctcg cggacacgcc ccccatggtg
    14701 ggatcggtga gcagggtgat gaagggcagc cggcgatccc ccagccgggt aatggcggcg
    14761 gtggtcttgg ccatctgcat gagggagaag agcccttcct gcatgcgcgc gccgcccgtg
    14821 gcggttgcgc agatgaatgg cagacgctgc tccagcgcgg cccgcacgcc gcgcacgaag
    14881 cgctcgccaa ccaccgagcc catggagccg ccgaggaatt caaactcgaa gcaggcgacc
    14941 accaccggca cggtcttgat ggcaccctgc acgacaacca gcgcgtccgc ctcaccggtg
    15001 tcctcgttgg cggccttcag acgctcggga tagcgcttgg aatccctgaa cttgagggga
    15061 tcgacgggca ccacctcggc cccgatctcg aagcggcctt ccggatcgag cagcagatcg
    15121 atgcgctggc gggcgcggat gcgctggtgg tgtccgcact tggggcagac gttctggttg
    15181 ctttccagat cggagcggta cagcacggcc tcgcaggccg ggcatttgct ccacagccct
    15241 tcgggaatcg ccttgcgcga cgaggtgtcg cgcttgatct tgggcggcag cagtttggtg
    15301 agccagctca tcgggcctcc cctcgttggt ccatggcctc acgcacaccg cgcaggaagg
    15361 ccgtcacccg cgccggagcg gcatcgcggg ggctgttttc aatctcttcg atgatgcggc
    15421 tgccgatcac caccgcgtcg gccagaccgg cgatgcgcgc ggcggtggcg gcgtcacgga
    15481 tgccgaagcc cacgcccacc ggcatgccga ctcgctcgcg gatcgccggg atgcggcgtg
    15541 ccacctcgtc caggtccagc gcggccgagc cggtcacgcc cttgagggac acatagtaaa
    15601 tgtagccgct gccggccgcc gccacgtcgg caaaacgctg ctcggtggac gtaggcgcca
    15661 gcaggaagat cgggtcgagc cccgcggcgc gacaggtggc cgcgaactca cggcattcct
    15721 ccggcgggta atccacgacc agcacgccgt cggcgcccgc ctcgaccgcc gcacgggcga
    15781 aggtgtcgac acccattgcc tcaatgggat tggcgtaacc catcaacacc accggcgtct
    15841 ggtcgcccgt ggcccggaag gcacggacga tgtcgagcac cttgcgcagg ctcatgcccg
    15901 ccgccagggc ccgttcggac gcgcgctgga tcgtcggacc gtccgccatg ggatcggaaa
    15961 agggcacgcc cagctcgatg atgtcggcac caccggccac cagggcctgc aacagaggca
    16021 ccgtcatctc cggggcggga tcgccggccg tgatgaaggg gatgagcgcc ttgcgcccgt
    16081 cggcggctaa acgctggaaa cagttctgga ttcgcgacat ggatgatggc gtggaatcgg
    16141 ggcggacagc tcgggccgtc cgccgggttc ggttcagaaa cggatgccgg acttctcggc
    16201 gacggtgtgc atgtccttgt cgccgcgacc ggacagattc acgagcagga tcttgtcctt
    16261 cggcagggtt ggcgccagct tggcagcgta ggccagggca tgggaggact ccagcgccgg
    16321 gatgatgccc tcgaagcggc acaggtcgtg gaaggcctgc agcgcctcgg catcggtgat
    16381 gcccacgtat tcggcgcggc tgctgtcctt gagccaggcg tgttcgggcc cgaccccggg
    16441 gtagtcgaga cccgccgaga tggagtgggt ctcgatgatc tggccgtcct cattctggag
    16501 caggtaggtg cggttgccgt gcagcacccc cggccgcccg gccgacaggg aggccgcatg
    16561 ctggccgctg tcgaggccgt gcccggccgc ttcgacgccc accagacgca cgtcgggaaa
    16621 gccgatgtag ggatggaaga tgcccatcgc gttggatcca ccgccgacgc aggcgatcac
    16681 cgcgtccggc tgacgccccg ccagaggcgg catctgctcg atgcattcct gaccgatgac
    16741 ggcctggaag tcccgcacca tcatcgggta gggatgcggc cccgccaccg tgccgatgat
    16801 gtagaaggtg ttgccgacgt tggtcaccca gtcgcgcatg gcctcgttga gggcatcctt
    16861 gagggtcttg gagccgctct ccaccggcac caccgtcgcg cccagcagct tcatgcgata
    16921 gacgttggct gcctgccggc gcacgtcttc ggagcccatg tacaccacac actccatgcc
    16981 atagcgggcc gccacggtgg cggtggccac gccgtgctga ccggcgccgg tctcggcgat
    17041 cacgcggggc ttgcccatgc gccgggcgag cagcgcctgg ccgatacaat tgttcacctt
    17101 gtgggcgccg gtgtggttga gatcttcccg cttcaggtag atctgcgcgc cccccagcag
    17161 gccggaccag cgcttggcgt ggtagatcgg gctgggacgc cccacgtaat gcttgagttc
    17221 gtactcgaat tcggcaatga actctggatc ctgttgcgcg gcggcatagg cttcgcgcag
    17281 ttcggccagc gccgggatca gcgtctcgga cacgaaaacg ccgccgtagg ggccgaaatg
    17341 gccgcgggcg tcgggaaggt cgtaaggggc atcagccatg tgcatgtcgg actccggcta
    17401 caaattcggt gatccgggcg gcgtccttga tgcctttggc ggcttccacg ccgctggaga
    17461 catccacggc ccacggccgc acgcgccgga tcgcttcacc cacgttgccg gcatgcagcc
    17521 cccccgacag gatcaggggc agcggcaggc cggacgggat gagggaccag tcgaaggttt
    17581 cgcctccccc accataagct tccacaaacg cgtccagcaa cagcccctgg gcgcccggaa
    17641 agcgagccgc gtattttaac agatcgagcc ccggccgcat cctcgccgcc ttgacatagg
    17701 gtcgaccgaa gccggcacag tccgcctcct cctcgtcgcc atggaactgc aggagttgga
    17761 tcggcacctg gcgcagggcc tcgtccacct cggcgcgcgg ggtgttgacg aacaggccga
    17821 cgacggtcac gaaaggcggc acgcgccggg ccagttcggc cgcccgcgcg aagctcacat
    17881 ggcgcggact gggcggatag aacacgaaac cgatggcgtc ggccccggcg ccgacggcgg
    17941 cgtccacgtc ttcttcgcgg gtcaggccgc agatcttgat tcgggttcga tgcacggggt
    18001 caccccagcg gaagttgcgg gggcacgatg atacgcccgc caccgggcag cggccaccgt
    18061 ggcggatact cgaccccaca caggtacagg ccgtccggcg cgaaggtggg tgccgccacg
    18121 ctgcggttgc gcgcggccag cacgtccgcc atccacgcca ccggcttgcg tcccctgccg
    18181 accatcacca gcgctccgac cagattgcgg atcatgtgat gaaggaaggc attggcatgg
    18241 aaatcgaaca ccaccagatc gcctacccga cccaccgcca cccgatacac cgtgcgcacc
    18301 ggcgacttcg cctggcactc cgcggcccgg aaggaggtga aatcgtgctc acccaccagg
    18361 cagtcggccg ctgccgtcat ggcctccacg tcgagtccat cgtgcaacca ccccacccga
    18421 cctgccagca gcgccggacg gacccgtcga ttgagcaaca gatagcggta gtggcgcgac
    18481 actgcgctga agcgcgcgtg aaaatcggcg gccactgtct gcgaccacag cacgctcaca
    18541 ccgcgcggca gatggctgtt gacgccccgc acccacgctt ccggcgggcg gaccgcggcg
    18601 gtgtcgaaat gcgccacctg cgtggtggca tgcacgcccg tgtcggttcg cccggcgcac
    18661 accagatgga tcggcgcctg ggcaatcaca catacggccg cttcgagcac gtcctggacc
    18721 gtgcgtccat ggggctgggt ctgccagcct tcgaaggcat caccggcata ttcgacaccc
    18781 aaagcgattc ggatcggtgg catatcaggc aaacatccat gccgcgacgc caatggccag
    18841 gacgctccag acgaatacat ccggccaacc gggcctcacc ttgggcagta cgatgacccc
    18901 aggctcggca ggccgctcgt cctcgaccag aaaggcgcgc cagccacgaa cgggcggcgc
    18961 gctggcgtac tgcaacacca gcaacatgcg caccgccagc cgatccgccg acagtcccag
    19021 cacatgcagg gggcgcgcca gactcgtcag cccccctacc agcgccgcct gcccggtctt
    19081 ctccagcagc agcgccacca ggaccaccac gctcaccagg cgcagaccat gcagcccggc
    19141 ggccgccaaa ccttcccgtg tcgggccggc ggcccccagt ccgggcagca gtgcctcacc
    19201 cggcgtgaaa aaagcgaaca ggatcaccag cacaccgagc aggatgcgca cccgacgaag
    19261 caagcgggca catcgcgtcc gggccgccca gaatcccgcg accaaaacaa acagcccgat
    19321 gacccctaca aagggccatc gggctatctg aaccactacc acccacgcag accacaggag
    19381 caggcgagtg gccggatgca acatcctctt gaagccgacg acgggagcaa tcagcccagg
    19441 cgggcgagaa tcgcctgcgc ttcgtccttc tgccgaccat tgccctcacg cagcacttct
    19501 tcgaccagct cgcgagcacc gtccttgtca cccatctctt cgtaggcacg cgccagttcg
    19561 agcttggtcg cgacctcctg atgcagatcc atgtcgagcg gcggttcggt cggcggaccc
    19621 gcatccggca aatcgacctc aaccgtcggc ctgtccagat ccagatcgat cccgctcaaa
    19681 tccaccgcgg gcatgtcagt ctgagtcgcc tgccccggat gcacatcggc ctttgagcga
    19741 tcatccaggt cgaaatcgaa atcaagcagg ttgccgtcga agttcgtgcg ctccagatcg
    19801 atggtggccg aatcatttgg cggacgaata ccctcgtcgg cgagcgtggc cgtgagatcc
    19861 acgtcgaccg acggcgcgac cggcggattc gcgttccgct caccgaattc ggaactgatc
    19921 tcgaaatcca gatcgctggg atccggcaag ccaaaatctt cgccgacccg ctggggacgg
    19981 gtggacgtca cttccatggt gcgaccgaac tcatcgccga ccaccatggt ggcaccggcc
    20041 ccgcgcgtga cggactgttc aaatccgaga tcgaaatcga ggccgcctgc cgacgccgga
    20101 ccattcccgt cgcccgtcac gatcgttgcc agatttccgg agggactgga cgcctccagg
    20161 ttcagatcga aatcgagcga tgcgggcctg gacggcgcaa cgggctcggg cgcactgggc
    20221 gcgtgatggt cgagatcgtt gagcgaggtg tactggttga gttcccccgg caaggcccag
    20281 gtgtccttga gctgggacgg cgactccaac ggatcgtcgt ccccggaggg cgcgatgggg
    20341 gccggcgcag gcgctgggcg agccgacatt ggcggcgcct gacgcgcttc gccagcctga
    20401 acgaagccgg tggccggtgc catcgtcccc gcaatcgtgt ggggaccgag ttccgacacg
    20461 gcaggcaccg gaatggccga cgggccaggc atcgcacccg gaaccggaat ccggtagagc
    20521 ggactgtccg gatccacctt ccgccccagc gcagccgcct tttcccaatc ggggccgttg
    20581 ccgcctgtga gggaataaag ctcggtggcc gtggtctcga attgcttcag attcttgcgc
    20641 tgggcataga tctccaacaa cttgagatgc acgcccgtcc gccctggatc ggtcttgagc
    20701 gcgtcgagaa ggatctcctc ggcttgggca tcgcgcccgt aggccatgta gacgtccgcc
    20761 tcagccaccg ggtcaacgcc ttcatccgta tcgatggacg acagacccga ttgactgaaa
    20821 tcggtctgga tctggctgct gctgcccgtg tcgacacttt gtccaccggc acttccaaag
    20881 acggagctgg atccttcgcc aaactccgaa taggcgggcg tcgtcgtcgg cgcgccgttg
    20941 ttgcgccgtt tgccaaacag acgataagcc ccccagccca gcagaagggc ggcgattcct
    21001 ccacccccca gcagcagcga catgtcaggc cccgaagacg cctcggccga cgccggagcg
    21061 ggtgcaggcg gcgggggcgg cgcgggcttg acctccggtg caggcggggg cggcggcgga
    21121 ggcgccagat ccgccggctt ctcggcctca ggaggtttga tggccgcttc cggcggcgga
    21181 accactgcgg caggcggtgg caccggcgct ggcggcggag gaggcaccac ggcaggcgct
    21241 gcagccggct tggccgcctg atcctgagtc tgggcgagcc cttggctctt cagttcgacg
    21301 agccgttgca gttcacgaat acttttttca agttcggcca gccgggcatt ggcatctttc
    21361 agcgccttgt cccgtgacac cagatcttct tcaagcgcct gaagacgcga cgaaggtttg
    21421 tccgcaccgc cgccagcaag tgaagcggca tcggcaccct tggaaacctt cagctggtcg
    21481 gagccggccg gcaacccctg agccttgtcc tcgacacggg caccgatgcg accgctggcg
    21541 ctctgacggg ccggagcctc cttcggtgca gatgcagccg cctgctcagc cagacgctga
    21601 cgatagcggc cgaagtcgga cgactgggcc acgacctggc gcccgccggg tgccagctgg
    21661 cggacaccgt cggcgtccgg aatgcgcaga accttgccgg cctgaagacg attgatgtcg
    21721 tccgccgcaa aagcaccccg gttgccctgg aacagcgccg acagcatctg atcaaccgac
    21781 acgccgtcgg gacgcgtttc catggcgatg cggttcagcg tgtcgcccgg cttgacgaga
    21841 tagctgcgat ccgacgccgc cggcaccgcg gcaggacgcg acgcgggtgc acgacgcacc
    21901 gccgccgggg caggcgccac gccgggacgg gccatgtcgg aacggacttc aggcacggcg
    21961 acagcagcgg ccctgggcgc ccccgcatca ggcggatcga gcaggaaggt gtattcgcgg
    22021 atcaggcgcc cggccgacca gttgagttca acgagcatgt ctacaaatgg ctcgttgatg
    22081 ggccgatcac tgctcacccg caccaccgcg ccatttccac ggcgctcgac ggccatgcgt
    22141 aacccggtca cggaaggcga gtagtcaatc ccggcctgac ggaaggcatc cgcggaagcc
    22201 agtcgggcgg tgatgttctg caactcctcc ggcgtcgccg cgatctccac ttcggcgcga
    22261 aggggctgac ccaggctgct catgacggtg atgcgaccca gtcccgctgc cccgctgccg
    22321 agggatacgg taccgatcgc aagcgccatg agactcagcc ggagccgatt gttgagggtt
    22381 ttcttatgca ctacggcttc tcaccgaaat cgtcttgccg atgaacataa catcatgggt
    22441 ttggcgaggc aagttcaagt cttttgccaa gcctttccat acaggacatt gcatgaaaaa
    22501 aggcggcttg cgccgccttc attgccatca cgcttcgagc agcacacgca acatgcggcg
    22561 cagcggttcg gccgcgcccc agagcagttg gtcccccacc gtgaaggccg aaagatattc
    22621 gccccccatc gccagcttgc gcaggcggcc gaccggaacg gtcagggtgc cggtcacggc
    22681 agtcggcgtc aggtcacgca tggaggcttc gcgctgattg ggcaccacct tgacccagtc
    22741 gttggccgag gcgagcaggc cttcgatctc gtcgaggggg atgtccttct tgagcttgag
    22801 ggtcagggcc tggctgtggc agcgcatggc gccaatgcgc acgcaaaggc cgtcgatggg
    22861 caccgccggg ctgccgaagc cttcaccgcg gccgaggatc ttgttggtct cgacaccgcc
    22921 cttccattct tccttggact ggccgttacc cagatccttg tcgatccagg gaatcaggga
    22981 gccggccagg gggacgccga agttggacga ggggaaggcg tcgtcccgca tggtgccggc
    23041 cacttcccgg tcgatgtcga ggatcgccga ggccggatcg tcgaggagtt ccttcacgga
    23101 gcggttgagc tcgcccatct gggtgagcag ctcgcgcatg ttctgcgcgc cggcaccgga
    23161 ggcggcctgg taggtcatgg aagtggccca ttcgaccagg tcacgctgga acaggccgcc
    23221 cagcgccatc agcatcaggg acacggtgca gttgccgccg atccagtcct tgccgccctt
    23281 ggccagggag tcctggatga cgttgaggtt gaccggatcg aggatgatca cggccttcgg
    23341 gtccatgcgc agcgcggagg cagcgtcgat ccagtggcca ttccagccag cggcgcgcaa
    23401 ctgggggtac acctcgttgg tgtagtcgcc gccctggcag gtgatgacga tgtccatcgc
    23461 cttcagctcg tcgatgctgc gggcgtcctt gagcgccggc gcgtccttgc cgaagttggg
    23521 cgccttgccg cccggattgg aggtggtgaa gaacaccggc tcgatcagtg cgaaatcgtt
    23581 ttcctcacgc atgcgctgca tcaggacgga acccaccatg ccgcgccagc ctaccagacc
    23641 cactcttttc at
//
LOCUS       DAOQMM010000051        23365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_715_length_23365_cov_46.749550, whole genome shotgun sequence.
ACCESSION   DAOQMM010000051 DAOQMM010000000
VERSION     DAOQMM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23365
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_715_length_23365_cov_46.749550"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1937
                     /locus_tag="PLH85_09885"
     CDS             171..1937
                     /locus_tag="PLH85_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB64916.1"
                     /translation="MSSLVDREWLDDEEAARNTCILVVERDEASAEVFQAFLKTSGFG
                     NVVTTLGATAVLDLLYDLEPDLLLVDSGALRAPDGSDLLESIRATRAFRHVPVLVLAA
                     QADREMKLQVLQQGVLDVLIKPVDPAELVLRLRNVLLSKLRGDRATFRDDLTGLANRE
                     RYTDRLQWAIRYSKRYGAEGAVLHIDLDRFKQIVSALGWGVGDRVLRAIANNLAVCVR
                     DTDVIAQGGERERSVMLSRLSGNEFTVLLSGIDRADSAAVVADRILHLVQQPMQVAGR
                     ELITTCSIGIAVFPSDGNSAEEIISAAVNALQHAKREGGNTFRFFARALNDRARHRLA
                     LESDLRVAVDEGQLGLVYQPKVRLDGNRITGAEALVRWIHPQRGVINPMDFIPVAEEC
                     GLIHAVGDWVLRETCRQIRAWREQGLAVPRIAINVSVHQFRQAGFADGVAAVLAEYGL
                     EGSVLGVELTESAIMENTVESLGALQALKDLGIELSIDDFGTGYSSLGYLKRFPLHIL
                     KIDRSFMIGVGKDEHSAAIVSAIISIGHRLGLRVVAEGVDTARQLEFLASHGCDEHQS
                     FLFSRPLYPDDFVAFLKRHTSL"
     gene            2197..3963
                     /locus_tag="PLH85_09890"
     CDS             2197..3963
                     /locus_tag="PLH85_09890"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB64917.1"
                     /translation="MTERSLTSIQRWLESYCGQTAVVAAGVIVRSEGSADAVRMLAAW
                     PAQGDALQPLVGAAAAALTRRQAVTMVPPVALPDTGIVRIVALPIQHTGDAGALAIGL
                     RSAEDSVVQGFLGEVQRLGATLAQLLQAPDAAPLREAGRLLQLQSVLLGRARLAEAAL
                     AFANELAATFAFDRVAVGLVEDGACRIEALSHNADFDRGQDLLRCLAAAMDEAADQGC
                     VLVQPPRPGDRLRIQLAHAELAGRTGAGVCSIPLAHDGEVIGVLTLERVGLAPPLQDE
                     LAQCEHLAALVAPLIALRQQAERPWRARLAAAGRQTWQRIVRGEDPRLRTGALVAGAV
                     LLATLVVPLPYRVGAAARIEGAEQRVLAAPVDGYLRKASVRPGDAVKTGQVLAEMVDQ
                     DLMLEERKWQAELSQHENAFIAAMARADRPGFSISRAKAGEARAQLELVRSQLARMQI
                     VAPIDGLVIEGDLTQSLGAPVKRGDALLTLAPQSRHRLMVEVDERDIGRVAPGADGEL
                     NLAALPGDSLAFRVMRITPVAVQRNGNNVFEVEAAVDGGDALRPGMQGVARIDAGHRP
                     LVWTLTHRAWDWLRLTLFSWGL"
     gene            3965..6106
                     /locus_tag="PLH85_09895"
     CDS             3965..6106
                     /locus_tag="PLH85_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNB64918.1"
                     /translation="MTASLFSPHWYRVADLRPRLRAQVRPNRQRWRDQLWYVLADQAT
                     GRHHRINETAYRFIGRCDGRRTVQQVWDAVLDADGDAAPTQDEVVELLTRLYESELLQ
                     SERSPDVDALFQRQSERAAGQRRATVNPLAFRLALGDPHRWLVRLDPLAHAVFRPGVF
                     GLWLVLMGVALITAGLHWPELRDHAARHVSSPRYLALLWLCYPVIKALHELGHALAVR
                     RWGGEVHEAGVVLLALVPAPYVDASAASGFLGRGPRAIVGAAGILVELALAAGGLFLW
                     LNTAPGWINDIAFVVMTVGSVSTLLINGNPLLRFDGYHVLCDALDLPNLAPRSTLYWS
                     DLLRRRVLRVAGEPPQMGAGEAKWLRLYAPLSALYRLAVGVSIMLWLGSKWFVLGVVA
                     AAYLAVAMLFRPVVGWVRQSLAAAAPGPALVRAHRGLLLLLGVPGLLLFVVPMPYVSV
                     APAVVWLPDRAQVRPDVDGFVSELPVRDGQNVAPGELLAVLRNDDLAAERERLDSRLQ
                     GLRSEQFRLLLRDPVGAQNQAEEIARTEAELTRADERLAGLQVRAQAGGRLVMPRQAD
                     LPGGFVRKGQVLGYVFPDGDTLVRAAVPERDGHRVRLAGSTARVRLVGGGAELEARVR
                     TDQPAATRKLPSPALGERAGGPYPVEPGDEDGTLALEPVFLVDLSLPGHALADVGRRA
                     WVRFDLGYAPLAVQAYRHAAQLFLKHFNPTE"
     gene            6268..8097
                     /locus_tag="PLH85_09900"
     CDS             6268..8097
                     /locus_tag="PLH85_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB64919.1"
                     /translation="MGRFVRAVRRREGELASAGPAQFDAAVTALRLTLGRDGLGPGGL
                     VEAFAVLSVVCAGVLGVRPYDTQLVAARIVLDNSLAEMATGEGKTLAVALAAATAALA
                     GVPVHVITANDYLVSRDAAALEPLYAALGLRVGAVVQSMGADARRDAYACDVAYCTAK
                     ELVFDYLRDGLERTRDRLRWHVQQLCGDGGTAPVLRGLCMAIVDEADSILIDEARVPL
                     ILSRPVAQVQQDRYLGQSLALARSLEAERDYRLEPGPRSAVLTPAGADRVARAADVLG
                     AVWHNRLHREEVIGLALTALHLYRRDRDYLVRDGAVQIVDEHTGRVAPGRAWSRGLHQ
                     LLELKEGCAPTPPTETLAQITYQRFFPRYLRLGGLSGTLREARAELLALYGLPVRGVP
                     LRGPDRRVTLPSRLFADSAALWSAVAARAASVAGQGRPVLVGTATVADSEALSARLAA
                     AGVDHVVLNARQDGDEARVVAEAGLAGRITVATNMAGRGTDIALAAGCAERGGLHVIV
                     CQLNGARRIDRQLAGRCARQGDPGSVETWLAADMPLLHDALPAALRRGLQRWAPRVPD
                     AALKGLRRLLQGREERLQALQRRRLRARDRYLERGLSFRGVSE"
     gene            8262..9584
                     /locus_tag="PLH85_09905"
     CDS             8262..9584
                     /locus_tag="PLH85_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB64920.1"
                     /translation="MTSKRKGWRYGVGLCLMAGVETALGGQSFGCLIEPERTAEIGSQ
                     VVGIVESFAVERGAFVSKGQPLAVLQAQTERAAVSLAKVKRDADAEVRAAESALDFAR
                     AGQTRADDLFNRNFISAHARDQARTDAEVAAQKLAQAREHRRIADGELEYAQAQLAQR
                     IIRSPFSGVVTERYLSPGERVEEKPLMKLAQIDPLKVQVVAPVAMYGRIEVGRSVQIT
                     PEMPEGKPVEARVTDVDRVLDPASNTFRVVLRLPNPRMELPAGLRCNADFGATGAAAG
                     GGMTAPRPVLPAPAPAAGAALRSGGTPAYDAPALIHASIAQRPAVAAPSPSLQAAALA
                     ALDDWLSAWSRRDVEQYLARYASDFRPAGGRSRAGWEAERRQRIGQAVHIEVRAEAPQ
                     VEVLGPEQVRVVFRQHYRSDAYAGVDAKRLTLVLQGGRWLIQREESGR"
     gene            9666..>23365
                     /locus_tag="PLH85_09910"
     CDS             9666..>23365
                     /locus_tag="PLH85_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNB64921.1"
                     /translation="MAAVIARPPHRRRPVVEELEARILYAADLAPFVHPLTDGAQVRV
                     LEAPAATPAPTLVEQAATTRHEIVFVDPGVADWTRLVADLQAQQDADRQLDVVLLDAG
                     RDGVAQITGALQGLHDIAAIHLISHGEAGAVMLGSTRLDLASIDAEAARIADWAPALQ
                     AGADILVYGCDVAASADGQALLARLRALSGADIAASTDLTGAARLGGDWTLEYSLGAI
                     QARVAADVPTQADWQGVLATYTVTNTNDSGAGSFRQAIQDANANPGADTINFNIAGTG
                     IHTITLSSMLPYLSGQVNIDATTDDSFAANGNRPAIAIDGDNTIQDGLELYGGSDGSV
                     VRGLIIERFTQDGIDIASSGNNTVAGNWIGLNTAGTAAAGNQQGVNIWSANNNTVGGT
                     TAADRNVIAGNTGPGIFVGGGSTGTHIYGNYIGTDKTGTVAIANQVAGVELMSSGTTV
                     GGTTSAYRNLISGNTVGVAFYAGGSSNTVLGNYIGTDVTGSVALANVNYGVQINSDAS
                     YNVIGGTSAGARNLISGNTNYGVAIMDPGAVGNQVEGNWIGVNAAGTAALSNLVGVIL
                     YNSTSGNTVGGTSAGAGNVISGNSVDGIQIFGSATGNTIQGNLVGLNPGGTSAIANTN
                     QGIALFGGASNTLIGGTTSAARNVISGNGNEGIRIEDAGTTGNLVQGNYIGTNAAGSA
                     GVGNAVGVHVFNGATGNTVGGTAAGAGNLIAYNSGIGVSVTDSTTTGNVILSNSFTSN
                     DGLAIDLGADGVTLNDVGDADTGPNNLTNFADLGSATTTGTELRLTGALSVAANTYYV
                     IQVYSSPVGDPSGYGEGLTYLGAINVPSGPSGSVSGRYTLSVAVAAGSVITSTATRTD
                     SSYTTYYETSEFSNQVVALAPGVTVTPVSTTTTEAGGTASFTVVLNAAPNATVTIPVS
                     SSDTTEGSVSASSLSFTTSNWNTPQTVTVTGVDDTIVDGTVAYSAVLGAATSSDAAYN
                     GLNPADVSLSNTDNDTYNTLTVTTAADTLDGTTTSIAALMANMGADGKISLREAITAA
                     NNTANGSGGPDRIAFGISGTGLHTINLGSALPNITDVVVIDGTTDDSFAANGNLPAIA
                     LNGASVTSGDGLHLMSGASGSTVRGLVIQGFANGYGVNIDNSANNQIVGNYIGTTLAG
                     TAAVANFRGLYLSGAAATGNVIGGSTAADRNLISGNSNDGVSINGASLNTVIGNIVGL
                     NAAGTAALGNGAQGIGIWGGAAQNTVGGTSAGLRNVISGNLGDGIRLDASTNNTVAGN
                     YIGLASDGSTAVGNGEVGVNITNGATGNLIGGSTSASRNVISGNTQYGIYVDAQIGAT
                     ANTTIQNNYLGTNAAGSAAVGNGGFGLAIDYGASNTSVLDNVISGNTATSWSASRGGI
                     YVYANGATIQGNLIGLDATGTSFIPNGGGSSAAGIYEAGGSTSVLIGGTGVGQGNTIA
                     GNTGSGVAVNASGNVRILGNAIYGNTGLGIDLDTNGVTANDAGDGDTGANHLQNFPVL
                     GTVQSNGTNQISIAGTLNSNASSYYRVEFFASTSADGTGYGEGQTYLGYVNVATNGSG
                     NASFSTVLSAVVPVGAYVSATATKSDASYATFTDTSEFALDVQAQTWNTAPVNTVPGA
                     QTVNEDTALTITGLSVADAESNLSSTRLTVSSGTLTVSLAGGATIGAGANGSASLTLS
                     GTPAQINAALATVSYLGTANFNGSDTLTILSTDSGALTDSDTVAITVTAVNDAPVLAG
                     GNAFTTITEDQTSSSGDLVSALIAGQVTDADSGAASGIAVTATVSSTGTWQYSLDNGS
                     TWNGVGSVSGNSALLLRSTDRLRFVPDGLNGTTASVTFRAWDQSSGSAGNKVDASTNG
                     GTTAFSTATATSNLSVTAVNDAPVLAGGNAFTTITEDQTSSSGDLVSALIAGQVTDAD
                     SGAASGIAVTATVSSTGTWQYSLDNGSTWNGVGSVSGNSALLLRSTDRLRFVPDGLNG
                     TTASVTFRAWDQSSGSAGNKVDASTNGGTTAFSTATATSNLSVTAVNDAPSMPGVALG
                     STPEDVAITWQVSTFAGYSSDVDAGALKGLAILVVDNSHGAWQYTLDGSNWTDIGTVS
                     ASHALLLAADATSAFRFVPAANWNGTTGMFVYEAWDQTTGTAGTFADASVTGGTSAFS
                     GPSGSTLTVTPVNDPPVITSNGGGSTASVSVPENTTAVTTVTATDVDSPSLSFSLAGG
                     ADAALFTVNANSGVLSFISAPNFEVPTDANGDNQYVLVVQASDGSSTTTQTLTVTVTD
                     VNEAPVVTSFGGASSAALSVPENQTAVTVVTATDPDTGANLTFSVTGGADAARFSIDP
                     SSGALVFNTAPMVAAPSDANGDGVYLVTVAVSDGTLTASQSLSITVTRDGGISVVPAI
                     ASAGTEVRVNTSTANTQYGPDSDNNSVAMNDNGDTVVVWMSMLQDGSGYGVYGQRYDA
                     SGAAVGGEFQINQTTAGNQQFPSVAMAADGSFVVVWDSDQSNGGDIFMRRYGADGTAL
                     SGEVRVNTVTTNLQQVANVAMDASGNFIVVWDSNASGNVDVRAQRYSSTGTALGGEFT
                     VNATLAGDQKTNSVAMDATGDFVVAWTGVNGDGSGNGVFAQRYNASGTAVGSQFQVNQ
                     ITTGNQDWAHVAMDGHGGFVVVWQSAASDTGDVYARRYDGNGFAVTNEFRVNVTPSGL
                     QNNPDVVMDAHGNFIVAWTSAGQDGSGNGVYGRRYSAGGVALGSEFLINVTTAGDQAF
                     PHMALEADGDFVVEWEGAGTGDADGVFLRRFSAQGVTTEAGGTASFQVVLDRAPSAPV
                     SIAVSSSNAADGTVSTASLVFTAANWNTPQTVTVTGVDNILVTPDASYTIVLGAAVSS
                     DSVYNGIDPADVVLTHHNDDIAVTTTADVVDGDTSSLNALITHPGVDGRISLREAITA
                     ANATAGTDTIRFSIPDALAGGAHTLSLATDLPTVTDAVVIDGTTEPDFAGTPVVVLDG
                     SGGALQGLEIAASGSTVRGLVIERFAQRGLLVDAGAANTRIVGNYIGTDVSGLLARGN
                     GAWGIDLVAAGSGTVIGGTTSADRNVIGANTGWGGIAINGTPAATVTGNYIGVGADGV
                     TALGNGIGVYLFNATAGSRIGGTAAGEGNLIGYNATGIAVQSAGSQAAILGNRYVANS
                     GQAIDLGWDGVALANDTGDGDTGPNGLQNLPVIVLARSDGSSLLSLTGSFNSLASTAF
                     RVEFYATPVNGGANGSGESLRYLGSVDVLTDASGNAPLAVGLGALVNPGEYVTATATR
                     SDAGHTVFYETSELARNVAVLSTVQATVVVDTTSDAADGNVTSLSTLLADKGADGRIS
                     LREAILAANATANGSGGADRIDFSIPDALVGGVHTIAVSAALPTVSEAALIDATTEPD
                     YAGTPVVVLDGSGAGSSADGLAISAGGSTVKGLTIRNFGHYGIYLTGGGGNLIAGNYI
                     GTDASGAAAAANHWGIVVDNSAGNVIGGTTAAARNLISGNLNDGINLSGSGATGNLVE
                     GNYIGTNAAGTAALGNAGVGVAVGAGASGNTIGGASASARNLISGNAEYGVFVSDPTT
                     SGNLIQGNWIGLDATGTTALGNGGFGVVVDNLAANTAILGNVISGNTAAGWSASRGGI
                     YLYANGATVQGNRIGTDASGNAFVANGGGSGMSGGIVGRETSANVMIGGTHLGEANVI
                     AGNTGDGVVVEGNATGYTVLGNAIHDNSGLGIDLGHDGVTANDAGDADVGLQNYPTLL
                     SVLSWGGNTVISGELRSQANTSYRVEFFANPAGGADPSGFGEGLTLLGFLDVTTDATG
                     YAAISTTLAGVSVAVGSRISATATSKTGATSYGATSEFGADVAVTPAGAVSVSAISGP
                     TAESGSTATFSVVLTIQPTADVTIALAVSDATEGSLSVSSLTFTAANWNTPQTVTVTG
                     VDDTWVDGNVAYSIVTGAAVSADANYNGVGVADVAVVNTDNDTYNTLVVDTVSDTADG
                     DTASIGALIANRGADGHISLREALLAANATANGSGGPDRIVFNIAGAGAHTVFLSGAL
                     PALSDAVLLDGSSQPGYAGAPLIELDGSGAGSSDGLVLGAGSDGSTLTGLAVNRFAGI
                     GIEVASSGNTLSADYVGLRPDGSTAAGNGSDGIRIDNASGNTISHSVVSGNLGAGIAL
                     IGAAASGNSVVGSIVGLDATGAAAVGNGLDGIVLSAGAHHNTLGSTTSGQGNVLSGNT
                     GSGIGLYTTHDNQIIGNLIGVDLGGTTALGNGQSGIAANQTVGEIVGGAAAGAGNTIS
                     GNAQNGVTLVDASGTVVQGNRIGLDVSGTTLLGNAGAGVALSGSTSGSLVGGTAAGAG
                     NRIAGNQIGVVIGGTSAGNAILANSMYGNTLLGIDLGWDGVTANDTGDGDTGPNGLQN
                     FPVLTYANSTGGNTTLAGSFNSAASTPYRLEFYASATGDASGHGEGQTFLGATTVTTD
                     ASGNAVFSVSLIGVSVANGAVVSATATADLGGGSYGGTSEFAANRSATTAGPGVTVTP
                     TAGLATSELGATASFSVVLDSAPVADVVIALSVSDATEGSLSVVSLTFTAANWNQAQT
                     VTVTGVADGIVDGNIAYTVITAQASSADAGYNGLDPADVSLTNFDANPPVITSNGGG"
BASE COUNT         3704 a         8071 c         8164 g         3426 t
ORIGIN      
        1 gccgggcgcg ccgtacggct cgtgacgcag ggcccacact cggcatcggc ccggttcaag
       61 atcggccggc cgaacccgtt agcgctggaa gtgagtctgc cgtcattgcc cgccatgccc
      121 tgttccccga tcgattgccg catcccctcc gcctgtcggg agattgcccg atgagttcgc
      181 tggtcgaccg ggagtggctc gacgacgagg aggccgcacg gaatacctgc atccttgtgg
      241 tggagcggga cgaggccagc gccgaggtgt ttcaggcctt cctcaagacg tccggtttcg
      301 gcaacgtggt gacgaccctc ggcgcaacgg cggtgctgga cctgctctat gacctggagc
      361 cggacctgct gctggtggac agcggtgcat tgcgggcgcc ggatggcagc gatctgctgg
      421 agtccatccg ggcgacccgc gccttccgtc acgtcccggt gctggtcctg gccgcccagg
      481 cggatcggga gatgaagctg caggtcctgc agcagggtgt gctggacgtg ctgatcaaac
      541 cggtggatcc ggcggagctg gtgctgcgtt tgcggaacgt gttgctgagc aagctgcgcg
      601 gcgaccgggc gaccttccgt gacgacctga ccggcctggc caaccgggag cgctacacgg
      661 accgcctgca atgggcgatc cgctacagca agcgttacgg tgcggaaggc gccgtgctgc
      721 acatcgacct ggatcgcttc aagcagatcg tgagcgccct gggctggggc gtgggcgacc
      781 gggtgctgag ggccatcgcc aacaatctgg cggtgtgcgt gcgtgacacg gatgtgatcg
      841 cccagggcgg cgagcgggag cgctcggtga tgctgtcgcg cctgtcgggc aacgaattca
      901 cggtgttgtt gagcggcatc gaccgggcgg acagcgccgc ggtggtggcg gaccgcatcc
      961 tccatctcgt gcagcaaccg atgcaggtgg ccggccggga gctgatcacc acgtgctcga
     1021 tcggcatcgc ggtgtttccg tcggacggca attcggcgga ggagatcatc tcggcggcgg
     1081 tgaacgcgct gcagcacgcc aagcgggagg gcggcaacac cttccgcttc ttcgcccgtg
     1141 ccctcaacga ccgggcgcgg caccggctgg cgctggaatc cgatctgcgg gtggcggtgg
     1201 acgagggcca gctggggctg gtctatcagc ccaaggtgcg cctggacggc aaccgcatca
     1261 ccggtgccga ggcgctggtg cgctggatcc atccccagcg gggcgtgatc aatccgatgg
     1321 acttcatccc ggtggcggag gagtgcgggc tgatccacgc cgtgggcgac tgggtgctgc
     1381 gcgagacctg ccggcagatc cgcgcctggc gcgagcaggg gcttgccgtg ccgcgcattg
     1441 ccatcaacgt gtcggtgcat cagttccgcc aggccggctt tgccgacggt gtggcggcgg
     1501 tgctggcgga atacgggctg gagggcagcg tgctgggggt cgagctcacc gagagtgcga
     1561 tcatggaaaa caccgtggag agcctgggcg cgctgcaggc gctgaaggat ctgggcatcg
     1621 agctgtccat cgatgacttc ggcaccggct attcgtccct gggctacctg aagcgctttc
     1681 cgctgcacat cctcaagatc gaccgctcct tcatgattgg cgtgggcaag gacgagcaca
     1741 gtgcggccat cgtctccgcc atcatctcca tcggccaccg cctcggcctg cgggttgtgg
     1801 cggagggcgt cgacaccgcc cgccagctgg aattcctggc cagtcacggc tgcgacgagc
     1861 accagagttt tctgttcagc cgtcccctct atcccgacga cttcgtcgcc ttcctgaaac
     1921 gtcacacgag cctttgaggc ggcaggggtg tcgggctgca ggcgcatgca gcgccggcct
     1981 caagcccgtg cgacggatgc cgataagcct ctgggtgccc ggtccgatga tctccgcggc
     2041 gccgggtgat ggcgcgtccg atccggcggg gagcccgatc ggattgccgg gaccttaaaa
     2101 cattggcgcc gcatttcacc gctctcctgc ccgccgcacg gcgatggccc cttcgaccgg
     2161 aagggtgccg ctgcgccgca cgggggcctg acggacatga ccgaacgtag tcttacctcc
     2221 atccagcgct ggctggagtc ctattgcggc cagaccgcgg tggttgccgc cggcgtgatc
     2281 gtgcgcagcg aaggcagcgc cgacgcggta cgcatgctgg ccgcctggcc ggcccagggc
     2341 gatgccctcc agcccctggt gggcgccgcc gccgccgccc tgacccgccg tcaggccgtc
     2401 acgatggtgc cgcccgttgc cttgcccgac accggaatcg tgcgcatcgt cgccttgccg
     2461 atccagcaca cgggcgatgc cggcgcgttg gccatcggcc tgcgcagcgc cgaagactcc
     2521 gtggtgcagg gcttcctggg cgaagtccag cgtctcggtg ccaccctggc ccagctgctg
     2581 caagccccgg acgcagcgcc cctgcgcgag gccggccggc tgctccagct gcagagcgtg
     2641 ctgctcgggc gtgcccgcct ggctgaagcc gcgctggcct ttgccaatga actggcggcc
     2701 accttcgcat tcgatcgggt cgccgtcggg ctcgtggagg acggcgcctg tcgcatcgag
     2761 gccctttccc acaatgccga ctttgaccgc ggtcaggatc tgctgcgttg tctggccgcg
     2821 gccatggacg aggcggccga tcaaggctgc gtgctggtcc agccgccccg tccgggcgat
     2881 cgtctgcgca tccagctggc ccatgcggag ctggccgggc ggaccggcgc gggcgtgtgc
     2941 agcattcccc tggcgcatga cggcgaggtg atcggcgtgc tgaccctgga acgggtcggt
     3001 ctggcgccgc ccttgcagga cgagctggcc caatgcgaac acctggcggc cctcgtcgcg
     3061 cccctcatcg ccctgcgcca gcaggctgag cggccgtggc gggcccgcct ggccgcagcc
     3121 ggacggcaga cgtggcagcg gatcgtccgg ggcgaggacc cacgcctgcg caccggcgcg
     3181 ctggtggcgg gggccgtgct gctggcgacc cttgtcgtgc cgctgcccta ccgggtgggc
     3241 gcggcggcgc gcatcgaggg cgccgagcag cgggtgctcg cggcgccggt ggatggctat
     3301 ctgcgcaagg cctcggtacg ccccggcgac gccgtcaaga ccggtcaggt gctggccgag
     3361 atggtggacc aggatctgat gctggaagag cgcaagtggc aggccgagct gtcccagcac
     3421 gagaacgcct tcatcgccgc catggcgcgg gccgaccgcc ccggcttctc catcagccgc
     3481 gccaaggccg gtgaggcccg cgcgcaactg gagctggtgc gcagccagct ggcacgcatg
     3541 cagatcgtgg cgcccatcga cgggctcgtc atcgaaggcg atctcaccca atccctcggt
     3601 gcgcccgtca agcgcggtga cgccctgctc accctggcac cccagagccg ccatcggctg
     3661 atggtggagg tggacgagcg cgacatcggg cgggttgcgc ccggggcgga cggcgaactg
     3721 aacctggcgg cgctgcccgg cgacagcctg gcattccgcg tcatgcgcat caccccggtg
     3781 gcggtgcagc gtaacggcaa caacgtgttc gaggtggagg ccgcggtgga cgggggcgat
     3841 gccctgcgtc ccggcatgca gggcgtggcg aggatcgatg cggggcaccg gcccctggtg
     3901 tggaccctca cccaccgggc gtgggactgg ctgcgtctga ccctcttcag ctgggggctg
     3961 tgagatgacg gcgtccctct tcagccccca ctggtaccgg gtggcggacc tgcggccgcg
     4021 tctgcgggcc caggtccggc cgaaccgcca gcgctggcgt gaccagctgt ggtacgtgct
     4081 ggccgatcag gccaccggcc gccaccaccg catcaacgag acggcgtacc gcttcatcgg
     4141 gcgctgcgac gggcggcgca cggtgcagca ggtgtgggac gcggtgctgg acgcggacgg
     4201 cgacgcggcg ccgacccagg acgaggtggt ggagctcctc acccggctgt acgaaagcga
     4261 actgctccag tccgagcgca gtcccgatgt ggatgccctg ttccagcggc agtccgaacg
     4321 tgcggccggt cagcgccgtg ccaccgtcaa tccgctggcg ttccgtctgg ccctgggcga
     4381 cccccatcgc tggctggtcc ggctcgatcc gctggcccat gccgtgttcc gccccggggt
     4441 gttcgggctg tggctggtgc tgatgggcgt tgccctgatc acggccgggc tccattggcc
     4501 ggagctgcgc gaccatgcgg cccgccacgt gagttcgccg cgctatctgg cgctgctgtg
     4561 gctgtgctat ccggtcatca aggcgctgca cgaactgggt cacgccctgg ccgtgcggcg
     4621 ctggggcggc gaagtccacg aagccggcgt cgtgttgctg gccctggtgc ccgcacccta
     4681 cgtggacgcc tccgcggcat cgggcttcct ggggcgcgga ccccgcgcca tcgtcggggc
     4741 ggccggcatc ctggtggagc tggcgctggc ggcgggcggg ctgttcctgt ggctcaacac
     4801 cgcgccgggc tggatcaacg acatcgcctt cgtggtgatg accgtgggca gcgtgtccac
     4861 gctgctcatc aacggcaacc cgctgctccg attcgatggc tatcacgtac tgtgcgatgc
     4921 cctcgatctg cccaatctgg cgccccgcag caccctctac tggagcgatc tgctgcgtcg
     4981 gcgcgtcctg cgcgtggcgg gcgagccgcc ccagatgggc gcgggcgagg ccaagtggtt
     5041 gcgcctctac gcgcctctgt cggcgctcta ccgcctggcc gtaggcgtgt ccatcatgct
     5101 ctggctgggg tcgaagtggt tcgtcctggg ggtggtggcg gccgcctatc tggcggtggc
     5161 gatgctgttc cggccggtcg tgggctgggt ccgccagtcc ctcgccgcgg ccgcacccgg
     5221 cccggccctg gtacgggcgc acagggggct gctgctcctg ctcggcgtac ccggcctgct
     5281 gctcttcgtc gtgccgatgc cgtatgtcag tgtcgcgccg gcggtggtgt ggctgcctga
     5341 ccgggcccag gtgcgcccgg acgtggacgg cttcgtgtcg gaactgcccg tgcgcgacgg
     5401 ccagaacgtg gctcccggcg aactgctggc ggtcctgcgc aatgacgatc tggccgccga
     5461 gcgggagcgt ctcgacagcc gcctgcaggg cctgcgctcg gagcagttcc ggctgctgct
     5521 gcgcgacccc gtcggcgccc agaaccaggc cgaggagatc gcccgcacgg aagccgagct
     5581 gacccgggcc gacgagcgcc tggccggcct gcaggtgcgc gctcaggcgg gcggccggct
     5641 ggtcatgcca cgccaggccg atctgcccgg aggcttcgtc cgcaagggcc aggtgctagg
     5701 ctatgtcttt ccggacggcg ataccctggt gcgtgccgcc gtgcccgagc gggacggaca
     5761 ccgggtgcgg ctggccggca gcacggcccg cgtccgcctg gtcggcggcg gggcggagct
     5821 ggaagcccgc gtgcgcaccg accagcccgc cgcgacccgc aagctgccga gtccggccct
     5881 cggggagcgg gccggcggcc cctatcccgt cgagcccggt gacgaggacg gcacgctggc
     5941 cctggagccg gtgttcctgg tggatctgag cctgccgggc cacgccctcg ccgacgtggg
     6001 ccggcgcgcc tgggtgcgct tcgacctcgg ctacgcgccg ctggccgtgc aggcctaccg
     6061 ccacgccgcc cagctcttcc tgaaacactt caaccccacc gagtgagggg ccggccggtc
     6121 gttcgtccat ccatgcgtgc cgcgtccctg cccgtccctg gccccctgtg gggcagctat
     6181 ccggaaaggc gcacgccagc ggagtcggcg cagcccttcg gacggcgttt ggccgatctg
     6241 ggacgacgcc tcgctggagc gcgtgggatg ggtcgtttcg tccgcgcagt gcggcgccgg
     6301 gaaggcgaac tggccagcgc cggtccggcg cagttcgacg cggccgtcac ggcgctgcgg
     6361 ttgaccctgg ggcgcgacgg tctggggccg ggtgggctgg tcgaagcatt tgccgtgttg
     6421 tccgtggtct gtgccggggt cctcggggtg aggccttacg acacccagct ggtggcggcc
     6481 cgcatcgtgc tcgacaacag tctggcggag atggcgaccg gcgagggcaa gaccctggcg
     6541 gtggccctgg cggcggcgac agctgcgctg gccggggtgc cggtgcacgt gatcaccgcc
     6601 aacgattacc tggtgagccg cgacgcggct gccctcgagc ccctgtacgc cgccctgggc
     6661 ctgcgcgtcg gcgccgtcgt ccagtcgatg ggggcggacg cacggcgtga cgcctacgcc
     6721 tgcgacgtcg cctactgtac ggccaaggag cttgtgttcg actatctgcg cgacgggctc
     6781 gaacgtaccc gcgaccgcct gcgctggcac gtgcagcaac tgtgtggcga tggcggtaca
     6841 gcgccggtcc tgcgcggtct gtgcatggcc atcgtggacg aggcggacag catcctcatc
     6901 gacgaggcac gcgtgcccct gatcctctcc cggccagtcg cccaggtcca gcaggaccgc
     6961 tacctcgggc agtccctggc gctggcgcgc agcctcgagg cggagcggga ctaccgcctc
     7021 gagccggggc cgcgcagcgc tgtcctgacg ccggccggtg cggatcgggt ggcccgcgcc
     7081 gccgacgtgc tgggcgccgt gtggcacaac cgcctgcatc gggaggaggt gatcgggctc
     7141 gccctgaccg cgctgcatct ctatcggcgg gatcgggact atctggtccg cgacggggcc
     7201 gtgcagatcg tggacgagca caccggacgc gtcgcgcccg gccgcgcctg gtcacggggc
     7261 ctgcaccagc ttctggagct gaaggaaggc tgtgcgccca cgccaccgac ggaaaccctg
     7321 gcgcagatca cctatcagcg cttctttccc cgctacctgc gcctgggcgg actgagcggc
     7381 accctgcgcg aggcacgcgc cgagctgctg gccctctacg gtctgcccgt gcggggcgtt
     7441 ccgctgcggg ggcccgaccg gcgagtcacg ctgccgtcgc ggctctttgc cgacagcgcc
     7501 gccctgtggt cggcggtggc ggcgcgggcg gccagtgtcg ccggccaggg ccggccggtc
     7561 ctggtcggga ccgccacggt tgcggactcc gaggccctct cggcgcgtct ggcggctgcg
     7621 ggcgtggacc atgtcgtgct gaacgcgcgc caggatggcg atgaagcgcg ggtggtggcg
     7681 gaggccggcc tggcgggacg catcacggtg gccaccaaca tggccggacg cggtacggac
     7741 atcgcgctgg cggccggctg tgccgagcgc ggcggtctgc acgtgatcgt ctgtcagctc
     7801 aacggggcgc gccgcatcga ccgccagctg gccggccgct gcgcccggca gggcgatccc
     7861 ggcagcgtgg aaacctggct ggcggcagac atgcccctgc tgcacgacgc cctgccggcc
     7921 gcactgcgac gcggactgca gcgctgggcg ccgcgggtgc ccgacgcggc actgaagggc
     7981 ctgcggcgcc tgctccaggg gcgcgaggag cgcctccagg ccctgcaacg gcgccgcctg
     8041 cgcgcccgtg accggtatct ggagcgggga ctgtccttcc gcggggtctc cgagtagcgg
     8101 ggagcgtcac ccgtcccggc ggattgtgac ggaagtcctc aattcagggg catgtccagc
     8161 cgttaaagcc aagcaggatg aaggccgttg ccgccctccg ggggcaggct gcggccggcc
     8221 gggccgacgc gggccggtcc ataacgatcg gatgcaagca tatgaccagc aagcgcaagg
     8281 ggtggcggta cggggtcgga ctctgtctga tggcgggcgt ggagaccgcc ctgggcggac
     8341 agtctttcgg ctgcctgatc gagccggagc gtacggccga gatcggcagc caggtggtgg
     8401 gcatcgtcga atcgtttgcc gtggagcggg gcgccttcgt gagcaagggg cagccactgg
     8461 ccgtgctgca ggcccagacc gagcgggcgg cggtgtccct ggccaaggtc aagcgcgatg
     8521 cggacgccga ggtgcgggcc gcggaatcgg cgctggactt cgcccgtgcc ggtcagaccc
     8581 gcgccgacga tctgttcaac cgcaatttca tctccgccca cgcccgcgat caggcccgga
     8641 ccgacgccga agtggccgcc cagaagctgg cccaggcgcg ggaacatcgg cgcattgccg
     8701 acggcgaact ggaatacgcc caggcgcagc tggcccagcg catcatccgc agccccttca
     8761 gcggcgtggt caccgagcgc tatctctcgc ccggtgagcg ggtcgaggag aagccgttga
     8821 tgaagctggc ccagatcgat ccgctcaagg tccaggtggt ggcgccggtg gccatgtacg
     8881 ggcgcatcga ggtggggcgc agcgtgcaga tcaccccgga gatgcccgag ggcaagccgg
     8941 tggaagcgcg cgtgaccgac gtggaccggg ttctcgatcc ggccagcaat accttccggg
     9001 tcgtcctgcg cctgcccaat ccgcgcatgg agctgccggc cggtctgcgt tgcaacgccg
     9061 atttcggggc gacgggcgcg gcggcgggcg gcggcatgac agccccgcgc ccggtgctgc
     9121 ccgcgccggc gccggcggcc ggggccgcgc tccggtcggg ggggactcct gcgtatgatg
     9181 cgcctgccct gatccacgca tccatcgcgc agcgtccggc cgtcgcggca ccctcgccgt
     9241 cgctgcaggc ggcggccctg gcggccctcg acgactggct gtccgcctgg tcacgacggg
     9301 atgtggagca gtatctcgcg cgctatgcca gcgacttccg gccggccggt ggccgttcac
     9361 gggccgggtg ggaagcggaa cgtcgtcagc gtatcgggca ggcggtgcac atcgaggtgc
     9421 gcgccgaggc gccccaggtc gaggtgctcg ggccggagca ggtccgtgtc gtctttcgcc
     9481 aacactatcg ctcagacgcc tatgcgggcg tggacgccaa gcgcctcacc ctggtcctgc
     9541 agggggggcg ctggctgatc cagcgggaag agagcgggcg ttgagatgaa gggccggatg
     9601 gcatgcgtac cgatctgcgg ggtcggcctc ccggacgccg tggggcgtga aggcggagct
     9661 gtggcatggc cgctgtgatc gcccgcccgc cccatcgccg gcggccggtc gtggaggagc
     9721 tggaggcccg catcctctac gccgccgatc tggcgccctt cgtccatccg ctcaccgacg
     9781 gcgcccaggt ccgggtcctt gaggcgccgg ccgcgacgcc ggcgcccacg ctggtggaac
     9841 aggccgccac gacccgccac gagatcgtct tcgtcgaccc tggcgtcgct gactggaccc
     9901 ggctggtggc cgatctgcag gcccagcagg atgccgaccg gcagctcgac gtggtgctcc
     9961 tggacgcggg gcgcgacggt gttgcccaga tcaccggggc gctgcaggga ctgcacgaca
    10021 ttgcggccat ccacctgatc tcccacggcg aggcgggcgc ggtgatgctc ggctcgaccc
    10081 gtctcgatct cgccagcatc gacgccgagg cggcccgcat cgcggactgg gcgcccgccc
    10141 tgcaggccgg cgccgacatt ctggtgtatg gctgtgacgt ggccgcctcg gccgatggcc
    10201 aggccttgct ggcccgcctg cgtgcgctgt cgggcgccga cattgccgcc agtaccgatc
    10261 tcacgggcgc tgcccgactg gggggcgact ggaccctgga atactccctc ggggccatcc
    10321 aggcccgggt ggcggctgac gtgccgaccc aggcggactg gcagggcgtg ctggccacct
    10381 ataccgtcac caacaccaac gattcgggcg ccggctcgtt ccgccaggcg attcaggatg
    10441 ccaacgccaa tcccggcgcg gacacgatca acttcaacat cgccggcacg ggcatccaca
    10501 ccatcacgct gagttccatg ctgccttacc tctcggggca ggtgaacatc gacgccacca
    10561 ccgatgacag ctttgccgcc aacggcaacc ggccggccat cgccatcgat ggggacaaca
    10621 ccatccagga tggtctggag ctgtacggcg gctccgacgg cagcgtagtg cgcgggctga
    10681 tcatcgaacg cttcacccag gacggcatcg acatcgcgtc gagcggcaac aacacggtgg
    10741 cggggaactg gatcggtctt aatactgcag gcaccgccgc agccggcaat cagcagggcg
    10801 tcaatatctg gtcggcgaac aacaatacgg tgggcggcac cacggctgct gaccgcaacg
    10861 tgatcgcggg caacaccggg ccggggattt tcgtcggtgg cggttcgacc ggcacccaca
    10921 tctacggcaa ctacatcggc acggataaaa ccggcacggt ggccatcgcc aaccaggtgg
    10981 ccggcgtgga actgatgtcc agcggtacca cggtgggggg cacgacctcg gcctaccgca
    11041 acctgatctc gggcaatacc gtcggcgtgg ccttctacgc gggtggcagc agcaacacgg
    11101 tgctcggcaa ctacatcggt accgacgtca ccgggtcggt ggccctggcc aacgtcaatt
    11161 acggcgtgca gatcaacagc gacgcctcct acaacgtgat tggcggcacc agcgcgggcg
    11221 cgcgcaacct gatctccggc aataccaatt acggtgtggc gatcatggat cccggcgcgg
    11281 tcggcaacca ggtggaaggc aactggatcg gcgtgaatgc ggccgggacc gctgccctgt
    11341 ccaatctggt gggggtgatc ctgtataact ccaccagcgg caacaccgtg ggcgggacga
    11401 gtgccggcgc cggcaacgtg atctccggca actcggtgga tggcatccag atcttcggct
    11461 ccgccaccgg caacaccatc cagggcaacc tcgtcggcct caaccccggc ggcacgagcg
    11521 ccattgccaa caccaaccag ggcattgccc tgttcggcgg ggccagcaac accctgatcg
    11581 gcggtaccac cagcgctgcg cgcaacgtga tttcgggcaa cggcaacgag ggtatccgca
    11641 tcgaggatgc gggcactacg ggcaacctgg tgcagggcaa ctatattggg accaacgccg
    11701 ccggctcggc aggcgtcggc aatgccgtgg gcgtccatgt cttcaacggc gccaccggca
    11761 acaccgtcgg cggcacggcc gctggcgccg gcaacctgat cgcctataac agcggcatcg
    11821 gtgtcagcgt caccgacagc accaccaccg gcaacgtgat cctgagcaac agctttacct
    11881 ccaacgacgg gctggccatc gatctggggg ccgacggggt caccctcaac gatgtgggcg
    11941 atgcggacac cggccccaac aatctgacca atttcgccga tctgggcagt gcgacgacga
    12001 cggggacgga gctgcgcctc accggcgccc tgagcgttgc tgccaatacg tattacgtca
    12061 tccaggtcta ttcgagcccg gtgggcgatc cgtccggcta cggcgaaggg ctgacctacc
    12121 tgggggccat caatgtgccg tccggcccca gcggcagcgt gtcgggccgc tacaccctga
    12181 gtgtcgcggt ggccgccggc agcgtgatca cctcgacggc cacgcgcacc gattccagct
    12241 acaccacgta ttacgaaacg tccgaattct ccaaccaggt ggtggccctg gcgccgggtg
    12301 tcaccgtcac cccggtcagc acgacgacca ccgaggcggg cggcacggcg tcgttcacgg
    12361 tggtgctcaa cgccgcgccg aacgccacgg tgacgatccc ggtgagcagc agcgacacca
    12421 cggaaggctc cgtgtccgcg tcgtccctga gcttcaccac gtccaactgg aacacgcccc
    12481 agacggtgac cgtcaccggt gtggacgaca ccatcgtcga cggcactgtg gcctacagcg
    12541 cggtgctggg ggcggccacc tcgtccgacg cggcctacaa cggcttgaac ccggccgacg
    12601 tcagcctcag caacacggac aacgacacct acaacaccct taccgtgacg accgcggccg
    12661 atacgctgga tggcaccacc acgagcatcg ccgcgctgat ggcgaacatg ggtgccgacg
    12721 gcaagatctc cctgcgggaa gccatcaccg cggccaacaa caccgccaac ggcagcggcg
    12781 ggccggaccg gatcgctttc ggcatcagcg gaaccggcct gcacacgatc aacctgggca
    12841 gcgcgctgcc gaacatcacc gatgtcgtgg tgatcgacgg gacgaccgac gacagcttcg
    12901 cggcaaatgg aaacctgccg gccatcgcac tcaatggggc ctcggtgacc agcggtgacg
    12961 gactgcatct gatgagcggc gcctcgggca gcaccgtgcg cgggctggtg atccagggct
    13021 tcgcgaacgg ctatggcgtc aacatcgaca actcggccaa caatcagatc gtcggcaact
    13081 acatcggcac caccctggcc ggcacggccg ccgtcgcgaa cttccgcggg ctctacctct
    13141 ccggggccgc tgcgacgggc aacgtgatcg gtggaagcac cgcggccgac cgcaacctga
    13201 tctccggcaa cagcaacgac ggcgtgagca tcaatggggc cagcctgaac acggtgatcg
    13261 gcaacatcgt gggcctcaac gccgccggca cggccgccct cggcaacggc gcccagggga
    13321 tcgggatctg gggcggcgcg gcgcagaaca ccgtcggcgg cacgtccgcc ggtctgcgca
    13381 acgtgatctc cggcaatctg ggggacggga tccgcctcga tgcctccacc aacaatacgg
    13441 tggccggtaa ctacatcggt ctggccagcg atggcagcac cgcggtcggc aatggcgagg
    13501 tcggggtcaa tatcacgaac ggcgccaccg gcaatctcat cggcggcagc acctccgcgt
    13561 cccgcaacgt catctccggc aacacccagt acggcatcta cgtggacgcc cagattggcg
    13621 cgacggccaa caccacgatc cagaacaact acctgggcac caacgcggcc ggttcggcgg
    13681 cggtcggcaa cggcggcttc gggctggcca ttgattacgg tgccagcaac acctcggtcc
    13741 tcgacaacgt gatctccggc aacacggcca caagctggag cgcgtcgcgg ggcggaatct
    13801 acgtatacgc caacggggcg acgatccagg gcaatctgat cggcctcgac gccacaggca
    13861 cgtccttcat acccaatggt ggcggcagtt ccgccgcggg catctacgaa gccgggggct
    13921 cgaccagcgt gctcatcggc gggacgggcg tggggcaggg caacaccatc gccggcaaca
    13981 ccggctccgg cgtggcggtg aacgcctcgg gcaatgtgcg catcctgggc aacgccatct
    14041 atggcaacac cggtctgggg atcgacctgg acaccaacgg cgtgaccgcc aacgacgctg
    14101 gcgacgggga tacgggcgcc aaccacctgc agaacttccc ggtgctgggc accgtccagt
    14161 ccaatggcac gaaccagatc tccatcgcgg gaacgctgaa ctccaacgcc agcagctact
    14221 accgtgtgga gttctttgcc agcaccagtg ccgacggcac gggctacggc gaggggcaga
    14281 cctatctcgg ctacgtcaat gtggccacca acgggtcggg gaacgccagt ttcagcaccg
    14341 tcctgtccgc ggtggtgccg gtcggcgcct atgtcagcgc cacggccaca aaaagcgacg
    14401 ccagctatgc caccttcacc gacacttcag aattcgccct cgacgtccag gcccagacct
    14461 ggaacacggc cccggtcaat accgtgcccg gcgcccagac ggtcaacgaa gacaccgccc
    14521 tgacgatcac cggcctcagt gtcgcggatg ccgagagcaa cctgagcagt acacgactca
    14581 ccgtgagcag cgggaccctc accgtcagcc tcgcgggcgg cgcgaccatc ggcgccggcg
    14641 ccaacggatc cgcaagcctg accctgagcg gcacccccgc ccagatcaac gctgcgctgg
    14701 ccaccgtgag ctacctgggc acggccaatt tcaacggttc ggatacgctc accatcctgt
    14761 ccaccgattc gggtgccctg acggacagcg atacggtggc gatcaccgtg acggcggtga
    14821 acgacgcccc ggtcctggcc ggtggcaacg ccttcacgac gatcaccgaa gaccagacca
    14881 gcagcagcgg cgacctcgtc tccgcgctca tcgccggcca ggtgacggac gcggacagcg
    14941 gcgcggcgag cggcatcgcc gtaaccgcga cggtcagcag caccggcacc tggcaatact
    15001 ccctggacaa cggcagcacc tggaacggtg tgggcagcgt ctcgggcaac tcggccctgc
    15061 tgctacgctc gaccgaccgg ctgcgcttcg tgccggacgg gctgaatggc acgacggcgt
    15121 cggtgacctt ccgtgcgtgg gaccagagca gcggctccgc cggcaacaag gtggacgcct
    15181 cgaccaacgg cggcaccacc gccttcagca ccgcgacggc cacctcgaac ctcagcgtga
    15241 cggcggtgaa cgacgccccg gtcctcgccg gtggcaacgc cttcacgacg atcaccgaag
    15301 accagaccag cagcagcggc gacctcgtct ccgcgctcat cgccggccag gtgacggacg
    15361 cggacagcgg cgcggcgagc ggcatcgccg taaccgcgac ggtcagcagc accggcacct
    15421 ggcaatactc cctggacaac ggcagcacct ggaacggtgt gggcagcgtc tcgggcaact
    15481 cggccctgct gctacgctcg accgaccggc tgcgcttcgt gccggacggg ctgaatggca
    15541 cgacggcgtc ggtgaccttc cgtgcgtggg accagagcag cggctccgcc ggcaacaagg
    15601 tggacgcctc gaccaacggc ggcaccaccg ccttcagcac cgcgacggcc acctcgaacc
    15661 tcagcgtgac ggcggtgaac gacgcgccat ccatgccggg cgtggccttg ggttccaccc
    15721 ccgaggatgt ggcgattacc tggcaggtct cgacctttgc cggctattcg agcgacgtgg
    15781 acgcgggggc gctgaagggg cttgccatcc tcgttgtgga caacagtcat ggcgcctggc
    15841 aatacaccct ggacggcagt aactggaccg acatcggcac cgtctcggcc agccatgcgc
    15901 tgctgctggc ggccgatgcc acgtcggcct tccgctttgt gccggcggcc aactggaatg
    15961 gcacgaccgg tatgttcgtc tacgaggcct gggaccagac cacgggcacc gccgggacct
    16021 ttgcggatgc cagcgtcacg ggcgggacgt cggctttcag cgggccgtcc ggatcgacgc
    16081 tgacggtcac gccggtgaac gatccgccgg tgatcaccag caatgggggc ggcagtacgg
    16141 ccagcgtgtc ggtgccggag aatacgaccg ccgtgaccac cgtgacggcg acggacgtgg
    16201 acagcccgtc cctgagcttt agccttgcag ggggcgcgga tgcggcgcta ttcaccgtca
    16261 atgccaacag cggtgtgctg agcttcatct ccgcgccgaa cttcgaggtg ccgacggatg
    16321 ccaatggcga caaccagtac gtgctcgtgg tccaggccag cgatggttcg tcgaccacga
    16381 cacagacgct caccgtcacg gtgacggacg tcaacgaggc gccggtcgtg accagcttcg
    16441 gcggggcctc cagcgcggcc ctcagcgtgc cggagaacca gacggcggtg acggtggtga
    16501 cggccaccga cccggatacc ggcgcaaacc tcaccttcag cgtcaccggt ggggccgacg
    16561 ctgcgcgctt ctccatcgat ccaagcagtg gcgccctggt gttcaacacg gcgcccatgg
    16621 ttgcggcgcc cagcgatgcc aatggcgacg gcgtctatct cgtgaccgtg gcggtctcgg
    16681 acggtacgct gaccgccagc cagtccctca gcatcacggt cacgcgcgac ggcgggattt
    16741 cggtggtgcc cgccatcgcg tccgccggca cggaggtccg cgtcaacacc agcaccgcca
    16801 acacgcaata cggccccgac tccgacaaca acagcgtcgc catgaacgac aacggggata
    16861 cggtggtggt gtggatgagc atgctgcagg acggcagcgg ctacggcgtg tatggccagc
    16921 gctacgacgc cagcggggcg gcggtgggcg gcgaattcca gatcaaccag acgacggccg
    16981 gcaaccagca gttcccgagc gtggccatgg cggcggacgg cagcttcgtg gtggtgtggg
    17041 actcggacca gagcaacggc ggcgacatct tcatgcgccg ctacggcgcg gacggcacgg
    17101 cgctgtccgg cgaagtccgg gtcaataccg tcaccaccaa tctgcagcag gttgccaatg
    17161 tggcgatgga cgcctccggc aacttcatcg tcgtatggga cagcaacgcc tccggcaatg
    17221 ttgacgtccg ggcccagcgc tacagcagca ccggcacggc gctgggcggc gagttcaccg
    17281 tcaacgccac cctggccggc gaccagaaga ccaactcggt ggccatggac gccacgggcg
    17341 attttgtcgt cgcctggaca ggtgtgaatg gcgatggcag cggcaacggc gtgtttgcgc
    17401 agcgctacaa cgcgtcgggc acggcggtcg gcagccagtt ccaggtcaat cagatcacca
    17461 ccggcaacca ggactgggcc cacgtggcca tggatggaca tggcggtttc gtggtggtct
    17521 ggcaatccgc ggccagcgat acgggcgatg tctatgcccg ccgctacgac ggcaacggct
    17581 ttgccgtcac caacgagttc cgggtgaatg tcacccccag tgggttgcag aacaatccgg
    17641 atgtggtgat ggatgcccac ggcaacttca tcgtggcgtg gaccagcgcc ggtcaggacg
    17701 gtagcggcaa cggggtctac gggcggcgct acagcgccgg cggggtcgcc ctgggcagcg
    17761 aattcctgat caacgtcacc acggccggcg atcaggcctt cccccacatg gccctggagg
    17821 cggatggcga ctttgtcgtg gagtgggaag gggctggcac cggcgatgcg gacggggtgt
    17881 tcctgcgccg cttctccgcc cagggcgtca cgaccgaggc cggcggtacg gcgagcttcc
    17941 aggtggtgct ggatcgggcg ccgagtgcgc cggtgagcat cgccgtgagc agcagcaatg
    18001 ccgcggacgg caccgtgtcc accgccagtc tggtatttac cgcggccaac tggaacacac
    18061 cccagaccgt gacggtgacg ggggtggaca acatcctcgt cacgcccgac gcgagctaca
    18121 ccatcgttct gggcgcggcg gtcagcagcg acagcgtcta caacgggatc gatccggcgg
    18181 atgtggtgct cacgcaccac aacgacgaca tcgccgtcac caccaccgcg gatgtcgtgg
    18241 acggtgacac gtccagtctc aacgccctca tcacccaccc cggcgtcgac gggcgcatca
    18301 gcctgcgcga ggcgatcact gcggccaacg ccacggccgg caccgacacg atccgttttt
    18361 cgattcccga cgccctggcg ggcggcgccc acaccctgtc gcttgcgacc gatctgccca
    18421 cggtgacgga cgcggtggtc atcgacggca cgacggagcc ggatttcgcc ggcacgccgg
    18481 tggtggtgct ggatggcagc ggcggcgccc tgcaaggtct ggagatcgcc gccagtggca
    18541 gcacggtgcg cgggctggtc atcgagcgct ttgcgcaacg gggcctcctg gtggatgcgg
    18601 gcgccgccaa cacgcgcatc gtgggcaact atatcggcac ggacgtgagc ggcctgctgg
    18661 cgcggggcaa tggtgcctgg ggcatcgacc tggtggccgc cggcagcggg acggtgatcg
    18721 gtggtacgac cagtgccgac cgcaacgtga tcggtgccaa caccggctgg ggcggcatcg
    18781 ccatcaacgg cacgcccgcg gcgaccgtca ccggcaacta catcggggtc ggcgcagacg
    18841 gggtgacggc gcttggcaac ggcatcgggg tttatctgtt caatgccacc gcgggctcac
    18901 gcatcggcgg cacggcggcc ggcgaaggca acctgatcgg ctacaacgcc accgggatcg
    18961 ccgtgcagtc cgccggttcc caggccgcca tcctcggcaa ccgctatgtg gccaacagcg
    19021 gccaggccat cgacctgggc tgggacggcg tcgccctggc caatgacacc ggcgatgggg
    19081 acacggggcc caatggcctg cagaacctcc cggtcatcgt gctggcccgc agtgacggca
    19141 gcagtctgct cagcctcacg ggcagcttca acagtctggc gagcacggcc ttccgcgtgg
    19201 agttctacgc cacgccggtc aacggcggcg caaacggcag cggcgagagc ttgcgttacc
    19261 tggggtccgt cgatgtcctc acggatgcct cgggcaatgc gcccctcgcc gtcggcctcg
    19321 gcgccctggt gaatccgggt gaatacgtga ccgccacggc cacccgcagc gacgcggggc
    19381 acacggtctt ctacgaaacc tctgaactgg cccgcaacgt ggccgtgctg tccaccgtgc
    19441 aggcgaccgt ggtggtcgac acaacctcgg atgccgcgga tggcaacgtc accagtctgt
    19501 ccacgctgct ggccgacaag ggggccgacg ggcgcatcag cctgcgcgag gcgatcctcg
    19561 ccgccaacgc cacggcgaac ggctccggtg gtgcggaccg gatcgatttc agcattccgg
    19621 atgccctggt cggcggcgtg cataccattg cggtgagcgc cgcgctgccc acggtgagtg
    19681 aggccgcgct gatcgacgcc accaccgagc ccgactacgc cggtacaccc gtcgtggtgc
    19741 tcgatggcag cggtgccggc agcagtgccg acggcctggc gatctccgcc ggtgggtcga
    19801 cggtcaaggg cctgaccatc cgcaacttcg gccactacgg catctatctc acgggcggcg
    19861 gcggcaacct gatcgccggc aactacatcg gcacggacgc cagcggcgcg gcggcggcag
    19921 ccaaccactg gggcatcgtg gtggacaact ctgccggtaa cgtcattggc ggcacgacgg
    19981 cggccgcgcg caacctgatc tcgggcaatc tcaatgacgg catcaacctg agcggcagcg
    20041 gtgcgacggg caacctcgtc gaaggcaact acatcggcac caacgccgcc ggcaccgccg
    20101 cgctgggcaa cgccggcgtg ggggtcgccg tgggcgccgg ggcgagcggc aacaccatcg
    20161 gcggtgcgtc ggcgtcggcc cgcaacctca tctccggcaa cgccgagtat ggcgtcttcg
    20221 tcagcgatcc cacgaccagc ggcaacctca tccagggcaa ctggatcggg ctggatgcga
    20281 cgggcaccac ggccctgggg aatggcggct tcggtgtggt ggtggacaat ctggcggcca
    20341 acacggccat cctcggcaac gtcatctcgg gcaacaccgc ggcgggctgg agcgcgtccc
    20401 gcggcggtat ctacctgtac gccaacggcg ccaccgtgca gggcaaccgg atcggcaccg
    20461 acgcgagcgg gaacgcgttt gtcgccaatg gcggcggcag cggcatgagc ggcggcatcg
    20521 tcgggcgcga gacgtctgcg aacgtgatga tcggcggaac ccacctgggc gaggccaacg
    20581 tgatcgccgg taacactggc gatggcgtgg tcgtcgaggg caatgccacg ggctacaccg
    20641 tgctcggcaa tgcgatccac gacaacagcg gcctgggcat cgacctgggc cacgacggcg
    20701 tgacggccaa cgatgccggc gacgcggacg tgggtctgca gaactacccg accctcctca
    20761 gcgtgctgtc gtggggcggc aacacggtca tcagcggtga actgcgcagt caggccaaca
    20821 ccagctaccg cgtggagttc ttcgccaatc ctgccggcgg cgccgacccg agcggtttcg
    20881 gcgaagggct gaccttgctc ggcttcctcg acgtcaccac cgacgccacc ggctatgccg
    20941 cgatcagcac gaccctggcc ggcgtgagcg tggcggtggg cagccgcatc agcgccacgg
    21001 ccacgagcaa gaccggcgcc accagctacg gggccacctc ggaattcggc gctgacgtgg
    21061 cggtgacgcc ggccggggcg gtgagtgtca gtgccatcag cggccccacc gcggaatccg
    21121 gcagcacggc caccttcagc gtcgtgctga caatccagcc cacggccgac gtcaccatcg
    21181 ccctggcggt gagcgacgcc accgagggca gcctgtcggt gagcagcctc acctttaccg
    21241 ctgccaactg gaatacgccc cagaccgtga cggtcaccgg tgtggacgac acctgggtgg
    21301 atggcaacgt ggcctattcc atcgtgaccg gcgcggcggt ttcggccgac gccaactaca
    21361 acggtgtcgg cgtggccgac gtggcggtcg tcaatacgga caacgacact tacaacacgc
    21421 tggtggtgga caccgtttcc gacacagcgg acggcgacac cgcctccatc ggcgcgctga
    21481 tcgccaacag gggcgccgac ggccacatca gtctgcggga agccctgctg gccgccaacg
    21541 ccacggccaa cggcagcggc ggcccggatc ggatcgtctt caacatcgcg ggcgccggcg
    21601 cgcacactgt gttcctctcc ggcgccctgc cggccctcag cgacgccgtc ctcctcgacg
    21661 gcagcagcca gcccggctat gccggcgcgc ccctgatcga gctggacggc agcggcgcgg
    21721 ggagcagtga cggcctcgtg ctgggcgccg gcagtgacgg cagcacgctt acgggcctgg
    21781 cggtcaatcg cttcgccggc atcggcatcg aggtcgcctc gtcgggcaac accctcagcg
    21841 ccgattacgt cggtctgcgt cccgacggca gcacggcagc tggtaacggc agcgacggca
    21901 tccgtatcga caacgcgtcc gggaacacga tcagccacag cgtggtgagc gggaacctgg
    21961 gcgccggcat cgccctgatc ggcgcggccg cgtccggcaa cagcgtggtg ggcagcattg
    22021 tcggcctgga cgcgacgggg gctgcggccg tcggcaatgg gctggacggc atcgtgctga
    22081 gcgctggcgc gcatcacaac accctcggct cgacgacgag cggccagggc aatgtgctgt
    22141 ccggcaacac cggcagcggc atcggcctgt acacgacgca cgacaaccag atcatcggca
    22201 acctgatcgg tgtggacctc ggcggaacga cggccctggg taacggacag tcgggcatcg
    22261 cggcgaacca gaccgtcggc gagatcgtcg gcggtgcggc agcgggtgcc ggcaacacga
    22321 tctccggcaa cgcgcagaac ggggtgacgc tcgtggacgc cagcggcacc gtggtgcagg
    22381 gcaaccggat cggcctggac gtgtcgggca cgacgctgct gggcaatgcc ggcgccggcg
    22441 tggccctgtc cggcagcacc agcggcagcc tcgtcggcgg cacggcggcc ggcgcgggca
    22501 accggatcgc cggcaaccag atcggtgtgg tgatcggcgg aacgagcgca ggtaacgcga
    22561 tcctcgccaa cagcatgtat ggcaatacct tactgggcat tgacctgggc tgggatggcg
    22621 tgacggccaa cgacaccggc gatggcgaca cgggaccgaa cggcctgcag aacttcccgg
    22681 tgctcaccta cgccaattcc accggcggca acacgaccct ggcgggaagc ttcaactccg
    22741 cagccagcac cccgtatcgc ctggagttct acgcctccgc cacgggtgac gcatccggcc
    22801 atggggaagg gcagaccttc ctcggcgcca cgaccgtcac gaccgacgcc agcggcaatg
    22861 ccgtgttcag cgtgtctctg atcggcgtga gcgtcgcgaa cggcgctgtc gtcagcgcaa
    22921 ccgccacggc ggacctgggc ggcggcagct acggcggcac gtccgagttc gcggccaatc
    22981 gcagtgccac caccgccggg ccgggtgtca cggtgacccc cacggccggg ctggccacca
    23041 gcgaactggg cgccacggcg agcttcagcg tggtgctgga cagcgcgccg gtggccgacg
    23101 tggtgatcgc cctctccgtc agcgacgcga ccgaaggcag cctgtcggtc gtgagcctga
    23161 ccttcaccgc cgccaactgg aatcaagccc agaccgtcac cgtgaccggt gtcgccgacg
    23221 gcatcgtgga cggcaacatc gcctacaccg tcatcaccgc tcaggccagc agcgccgacg
    23281 ccggctacaa cggccttgat cccgccgatg tgtccctcac caatttcgac gcaaatccgc
    23341 cggtcatcac cagcaacggc ggcgg
//
LOCUS       DAOQMM010000052        23341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_717_length_23341_cov_46.382290, whole genome shotgun sequence.
ACCESSION   DAOQMM010000052 DAOQMM010000000
VERSION     DAOQMM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23341
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_717_length_23341_cov_46.382290"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..889)
                     /locus_tag="PLH85_09915"
     CDS             complement(413..889)
                     /locus_tag="PLH85_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNB64922.1"
                     /translation="MKLPAFTLPSLVTRVGARLPQLPPTLALVAGLNAALDRLIPRDT
                     LEPLTGKRLLLRITDAGLALRFTLTPRGFRPVLDRRTPDLTISATTRDYLALALREED
                     PDTLFFSRRLLMEGETDLGLLVKNTLDAVDWEAVWRVMKSPIVGLTGASQTGSTVT"
     gene            complement(912..1817)
                     /locus_tag="PLH85_09920"
     CDS             complement(912..1817)
                     /locus_tag="PLH85_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNB64923.1"
                     /translation="MKLALGPLLYYWPRAATLSFYAEIAESPVDIVYLGETVCSRRHE
                     LRLADWLEVAETLAATGKEVVLSSQVLVESESDLKTLRRIVDNGRFRVEANDMGAVRL
                     LTAGDGARPDWIAGPTLNIFNPGTLGLFAGLGASRWVAPPELSRDQIAAVRAHLGLHM
                     ETEVFAHGRLPLAYSARCFTARHFNLQKDTCEFRCLQFADGIPLKTREGEPFLCLNGI
                     QTQSAHVHTLLGDLPAVVADGIDILRISPQGEHTAEVVTLFRDALEGLTPPQDALQEA
                     LPLLPEAPCNGFWHGRAGVEQFVTA"
     gene            complement(1835..2839)
                     /locus_tag="PLH85_09925"
     CDS             complement(1835..2839)
                     /locus_tag="PLH85_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNB64924.1"
                     /translation="MPAIELVCPAGSLPALKTAVDHGADCVYLGFKDDTNARNFSGLN
                     FDPKAMADGVRYAHDRGRKVLLALNTYPQTDTWQRWTGAVDRAAELGIDAVILADPGL
                     MAYARRVHPELRLHLSVQGSATSYEAINFYHAHFGIARAVLPRVLSLAQVEQVIAHTP
                     VEIEVFGFGGLCVMVEGRCALSAFATGESPNCNGACSPGKHVRWEQTPRGMETRLNGI
                     LIDRFADGERAGYPTLCKGRFEVNGETYYAIEEPTSLNTLELLPELARVGVRAIKIEG
                     RQRSPAYVAQVTRVWRAAIDRVLAGAGPFRVEPAWMAELNKVSEGQSHTLGAYYRPWK
                     "
     gene            3029..4021
                     /locus_tag="PLH85_09930"
     CDS             3029..4021
                     /locus_tag="PLH85_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB64925.1"
                     /translation="MTPNRRRFLTLGAGTLAMAMTGGGCSSGSSGPIKFATNPWPGYD
                     LLYVARDKGFFQDAGLDVNLVDLTSLGASRRAFERRQVDGCGATLVEILLAADLSGVR
                     PQIAAVVDYSDGADVVIGQAAMSSPTSLKGARVAIEPGTLHIQLLSLALARAGLTIRD
                     VTLVSMPQNVMPAAFAEGRVQAAVCFPPESGQLLARGGKVLFHSGQVPGEVVDILVFD
                     GDFVARRGADVRAFVRAYYQAQDYAARHPDEAHAIMARRENTSLADLRDTLKGLRYVD
                     QADQARLLEPGGPVERSLIHLQGAMIELAQLRERHTLKDFLMARELLAARGALA"
     gene            4018..7326
                     /locus_tag="PLH85_09935"
     CDS             4018..7326
                     /locus_tag="PLH85_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB64926.1"
                     /translation="MSGTRTLSLRWRLIGPLIGVWAVGLALLVTVLYINLLDRFRGLV
                     EQRADTLVAAVYSAAETAREPADLRRFVSTLGAGRDVSSVTLLGGRPARVIAATDHGL
                     HDLGVDELPDRPLAAQISRVILERKGERVLDAERRSLVVLEPVLLMGTDGEHGRLEDG
                     VLALRLDATSLWRAARDTVLHLSVGLGAIFSAVALGAYLMLSRYVLRPVDGIRDALDR
                     RAAGEADVPVPIARPDELGELARTLGELLDTLRIRNRQLVLARDHHLGMLESFPHLAW
                     RAGLNGQCDWFNRAWLRFTGRALAQEVGSGWLENVHPDDRDRCARAFRRHIDAREPMA
                     LEFRLRHADGTYHWIASHGAPMFDDTGEFVGHVGSCYDLQASRDNAQALARMTRLYHA
                     LSATNKAIVHTRDADALLPQVCRIAVEYGELERAWIGFRSAPGQGWRIVAAHGRNGPL
                     RLDDPALRCRLPDAGGASPHIDNDMAQGCGACGETDGGACATFPIAGDPELDAMPDGA
                     LFLRTSERNFFDVPKVALLEEMATDIGFALRNLQRDRALRLAARVFENNSEGIVVIAP
                     DRRVVMINRAFTTLTGFTLDAVRNLPLALFDPAHHPADFEADLRDHAAAGEVWQGEAW
                     LRRADGEAMPCALTLSAVRSDAGGLTHYVAVFSDISQRKADEARIRFLASHDFLTGLP
                     SRAVLEPTVADAIAAATRSGRRAALCFLDLDRFKNVNDTLGHHTGDRLLVEVARRLGA
                     VLQDGECLLRHGGDEFVVVMPEAGDRNALAAAAARLIGALDAPFELAGCEFSLSASIG
                     VAVFPEDGHEPDTLLDRADAAMYRAKEAGRNAVEFFADGMAAPTRERLALELALRRAL
                     ERQELAVHYQPLVKAHDSSLTGAEALLRWRHPELGDISPARFIPLAEESGLILPIGTW
                     VLDTVCRDLAAWRAAGRTVVPVAVNLSALQFRRADFVATVSDILHRHAIPAELLILEV
                     TESMVMRDVERAAHQLGELKALGLSIAVDDFGTGYSSLAYLKRFPLDKLKVDQSFVRE
                     INAGPGDRAIVAAIVGLGHTLGLSLVAEGVETEEMAETLRGLGCDLLQGWLFGRAEPA
                     AVFSERLAGALRYA"
     gene            7600..8487
                     /locus_tag="PLH85_09940"
     CDS             7600..8487
                     /locus_tag="PLH85_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64927.1"
                     /translation="MHDPSAPPSDDPDGLDALEAALSADPACIDAARKRVTAIRTTPV
                     PTAMVARDVHFPDVPAVTPPATTAPTTPPPKPGRGGGLLAELKQAVAEQKRVEADSSR
                     EEERVREVIGTALRRVFEYLHDLVGQLNQLRPPIPRSYTLLVTGTVFDGLSWQEGFTG
                     HRSQPHTNGDLLERVTLSYRLGGAGDTLIERTGSGIERLRQALFDFGFKTECTERRNA
                     RREVEFATFAVRSEISVQVQWRADFPRRQVIVEMRNLERLGTVEFALPAFALNPEFLD
                     EFGRMMLGQPNRFRELLPR"
     gene            complement(8580..10058)
                     /locus_tag="PLH85_09945"
     CDS             complement(8580..10058)
                     /locus_tag="PLH85_09945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB64928.1"
                     /translation="MDFSSLGLSEPIAQAVLEAGYTEPTSVQLAAIPAALAGKDLVVS
                     SRTGSGKTAAFMLPSLQRLTAEPTIKARGPRVLVLTPTRELAVQVTDATKRYGKNLRY
                     AKAVSVVGGMPYPVQNKLLSAPYEILVATPGRLMDQMNRGRIDFGRLEILILDEADRM
                     LDMGFVDDIEAICAALPKERQTLFFSATLDGQVARMMQRLLKDPERIEIATAQTRHDN
                     IEQRLLVADDIGHKHRLLDGILRDVAVDQAIVFTATKRDADALTLSLEAQGHSVAALH
                     GDMNQRMRTRTLDQLRRGHLRVLIATDVAARGIDVRRISHVINYDLPKVAADYVHRIG
                     RTGRGGDTGVAISLAERRDVRLVRAIERYTRQTMAVHTLPGLEPRASLPTEERRPAGP
                     RRDGRSFGNGPRFGDRRPREGGFGAPRFEGDRPARDSREGGHAHRAAGDARHDGPRRS
                     FGQPAPRSDARGGFGGARFGERPAGRADSRPRREGRTFDRDR"
     gene            10401..11267
                     /locus_tag="PLH85_09950"
     CDS             10401..11267
                     /locus_tag="PLH85_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNB64929.1"
                     /translation="MRFDNSRESDNIEDRRGDGGGGGLPIGGRGIGLGTVVLALVAMY
                     FGVDPRVVLNLAEQAPAPSAPAQHRAGPPANDPETVFVRHVLAETEDTWQTLFRQNGR
                     EYVDPKLVLFSGATRTACGTGQTAMGPFYCPADQKVYIDLAFYQELKSRFHAPGEFAQ
                     AYVIAHEVGHHVQNLLGISAKVQAARERAGEREANALSVRLELQADCLAGVWAKNADA
                     ARHIIEAGEVDQALRAASAIGDDTLQKQAQGYAVPDSFTHGSSEQRTRWFKRGMEAGD
                     LAACNTFQAKTL"
     gene            complement(11283..13742)
                     /locus_tag="PLH85_09955"
     CDS             complement(11283..13742)
                     /locus_tag="PLH85_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNB64930.1"
                     /translation="MRLVSCRPPPWLCAGCGRLLLLLVMLVGLVVPAVAQDEAHPGDT
                     VTRFRAALSAEERAWLDAHPVLRAGNGPDFQPFYAWQAGDRYAGPNADYLELLGRRTG
                     LQFRMVRFPEFPAVLHALDSGAIDLIPTLTPTESRRKRYRFTGGYMHSPAVLVTRSSG
                     GPTGLPADLRGVRIAIERGHASREVLLRTRPGARLVDVDDAASALQAVATGEADAYVG
                     LLAVAHYYMERLSLANLQVRQRFDADLSAMAMAVPRDQTLLAGILRKAMAFVSDAEGH
                     ALLRRYLPTGTGIPGEAFHLTAAEQAWLQAHGPIRVGYDQAFYPLSYTNLHRQAEGYS
                     IELFRLLRDKVGLSVDEVAGTWSAVLDKALRNELDVLVGVASTPERREKLLFVGPYLT
                     TPTAIVTHSDFQQVWDLGEFAGRKLALLESHFLRERIRSAYPTIQLVEVPTQEDALRR
                     VAAGSADAAIGNLYAINRLIQSHFLGKLYIAGHVPDGDSELYLGVQPAAPELAAILGR
                     ALEALSPADIATARNRWLDTRYMPELPVRRFALYLTVPLAALVILLAISAFMTRRLRR
                     ALEACQAREASLAEQFETLAGAHARMEAVTGAVARDLRRPVFSLDDQVDLLRDALVHA
                     PAKVPAALDRLAEASRRIRAALVGLEGLAGSAAPPPRRAPVNLAALATALWPDLMARH
                     GQGHAVRLDCAGGPQVTGDPVLLRGVVRQLLSNAVKFSRHTLDARVTLRTLPGGAHGF
                     EIADNGPGIPPAEVAALFQPYQRLSNAGRVEGAGIGLAVARQLARRMGGDIQLVAQAG
                     GGACFRVSLEVQDVVPAGRPV"
     gene            complement(13864..14337)
                     /locus_tag="PLH85_09960"
     CDS             complement(13864..14337)
                     /locus_tag="PLH85_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB64931.1"
                     /translation="MNRPFKVLGVQQIAIGGPSKDRLRTLWVDMFGLEVTGNFKSERE
                     NVDEDITAMGAGPFKVEVDLMQPLDPEKKPAVHTTPLNHVGLWIDDLPKAVEWLSANG
                     VRFAPGGIRKGAAGFDITFLHPKGNEEFPIGGEGVLIELVQAPPEVVDAFARLAG"
     gene            complement(14433..15233)
                     /locus_tag="PLH85_09965"
     CDS             complement(14433..15233)
                     /locus_tag="PLH85_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB64932.1"
                     /translation="MVPHASQAETRVAVLADCDNTQPPIVEYALRVAAQFGRVVLRRG
                     YGNHSTLARTWQEVLVRQAFTPCLQYTYAAGKNTSDIALALDALEILFDDRADKFCLV
                     TSDSDFAGLCRKLRERGATVCIVGEAKTPEALRNASDQFFEWAPAADTEAGNGTGPEQ
                     PAGAATKGEAARGGMIKRRPRFVVDAVALLAGNSSEGQVHLSALGQYLKRTDPGFSPA
                     NFGHSGLLDMLRTYDLLALHRADGGHYTVSLVPEADRVVARPAGSDTP"
     gene            15387..16244
                     /locus_tag="PLH85_09970"
     CDS             15387..16244
                     /locus_tag="PLH85_09970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB64933.1"
                     /translation="METPHRSDLAGTTALVTGASSGIGRVTARELACRGATVVLACRS
                     EARTQPVLAEIARLTGRADAAVWLPLDLADFASVRACAEGFLRLERPLHLLVNNAGLA
                     GARGLTRDGFEMAFGVNHLGHFLLTRCLEDRLITSAPARVVTVASRAHRGAGGLDWAA
                     LRRPTRSLTGIREYAASKLANILFSAALARRLADRGVGAYAVHPGVVDTEVWRTVPGA
                     LRPLLRLRGLLTPEDGARTSLHCCVDALPGESGLYFARSSPVTPSRRARDGALADELW
                     QRSEAWVAS"
     gene            complement(16268..17569)
                     /locus_tag="PLH85_09975"
     CDS             complement(16268..17569)
                     /locus_tag="PLH85_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNB64934.1"
                     /translation="MNPRFPDASCPALFVAAPASGQGKTTVTAALARLHARQGRRVRV
                     FKCGPDFLDPQIHAVASGHPVHNLDLGMCGSADAAWRLHAAAQDADLILVEGVMGLFD
                     GTPSGADIARYFGIPVMAVIDARAMAQTFGALVHGLATWEPGLPFSGVLANHVGSPGH
                     ADLLRDSLGGSFAWYGALPQDADAALPERHLGLLQAAEIADLGARLDRLADHLARTGA
                     ADLPPPVSFAGMPPADVPPLLAGRTVAIARDAAFGFIYPANLDLLRALGAELRFFSPL
                     AGESLPPCDALWLPGGYPELHAATLAAHDAWHADLAAHKAAGKPILAECGGMMSLFAS
                     ITDREGVTHPGAGLLPGHTRMQDRLAALGTQIVLLPEGRLTGHTFHYSRSETPLEPLA
                     RAHTPAGREGEAIYRVGRLTASYVHFYFPSNPTAAARLFGA"
     gene            complement(17566..18357)
                     /gene="cobA"
                     /locus_tag="PLH85_09980"
     CDS             complement(17566..18357)
                     /gene="cobA"
                     /locus_tag="PLH85_09980"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNB64935.1"
                     /translation="MSDSTLPPSRGAARPGWVYLVGAGPGDAELLTLRAARLLLEADA
                     LVYDNLVSKEVLACVPAGTARYYVGKKASNHSLPQEGINALLVSLAREGRRVVRLKGG
                     DPFIFGRGGEEVEELVEAGIPFEVVPGITAASGIGAYAGIPLTHRDHAQSCVFVTGHL
                     KDGTVDLDWDMLARPKQTLVFYMGITRLTDICSQLIAHGLAPTTPAAVVRHGTSPDQR
                     VVTADLATLPEAVKRAGITPPALTLVGSVVSLQGRMAWFNPEGGA"
     gene            complement(18394..>20328)
                     /locus_tag="PLH85_09985"
     CDS             complement(18394..>20328)
                     /locus_tag="PLH85_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB64936.1"
                     /translation="RMNDDQITPPAADPADASPPARRRAAPRAASRRKSADVPDTPEK
                     PVGPAAGDAPASPQGDAPPTLAEATPRKRAAGKAKSPRAPSRRAPRGSARPPEPDSLP
                     DTPPELPAARLAAPQAELVLESPAVRSDGAVVADEALPVVPREPAAADPESRPEPAAD
                     APDEAAASDDPTAPITAEGAVPEAGDESGGPRRKRKRRRGRGGSDTDGDAAVSDGEAA
                     DAALMDSAAEALAPSFADAIPEPAEPPPLTVVSLGLDLPPALSAAAREALAGARVILG
                     TAEALDRLSGLSLSAPQRLCGAETAHLDVRNAGHAGAVLVCVGDGAGDGPAAELLADL
                     GPAHVRVLPGVGRVQAACAALGLSAEIVTLVDLRRAPLAALRGQLRAHRLLAVPVADA
                     ERPAAVARLLLESGFPGARVWVCEFNGTAPRATAWLASELVELREPLDPHAVLIVATG
                     PSAGVFTELPGLSAAALGDDAGAALPLAVRVLALAWLQPAAWETGWAISDGEASVALE
                     WARAVPTARVRAVGVDPTLLGLSLARVGVGENLAAIPSAMPIHAAAWPAPEAVFIRGS
                     AGLPEWLAMAWARLAPGGRLVVSAEDDQARADLVTFAAQVPAEAWQELSQSRGECVAG
                     RLRFVPDAPVRLALWRKPAT"
     assembly_gap    20329..20428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20429..20959
                     /locus_tag="PLH85_09990"
     CDS             <20429..20959
                     /locus_tag="PLH85_09990"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNB64937.1"
                     /translation="AGKPLARNLKARVQTTALGQFPFASVVGCIDSRAAPEIVFDQSI
                     GDLFVARVAGNTVNEDILGSLEYAAKVAGSRVIVVLGHTSCGAIKGACDDVKLGNLTT
                     LLAKFKPAVAAATTPGERTSHNHAFVDEVTELNVKQVVQKIRADSPILKGLEDEGRIR
                     IVGALYDTSSGKVRWY"
     gene            complement(21050..>23341)
                     /locus_tag="PLH85_09995"
     CDS             complement(21050..>23341)
                     /locus_tag="PLH85_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HNB64938.1"
                     /translation="NEDVVIPLPRMGDYCDGIERINIELSIQNKLALCEALRAFLEGE
                     LPLHTGDATISREELLGDRRRDALNYLEAVRLRWEWLLENLDLPLAEAEPRFVSYGVV
                     AGELTNRAARPRLFHRLQDYSVRVSWKSELKPRLEKIFDGQMFAPVVEQLRAIHKQVL
                     RGRVFVALHMHAGDGNVHTNIPVNSDNYEMLQTANRAVDRIMALARALDGVISGEHGI
                     GITKLDYLTDAEMANFWAYKQQVDPEGRFNRGKLMRHGALTGNLDNAYTPSFNLMGHE
                     SLIMEQTAIGEISHDIKDCLRCGKCKPVCSTHVPRANLLYSPRNKILSTSLLIEAFLY
                     EEQTRRGVSLAHWAEFEDVADHCTVCHKCEKPCPVDIDFGDVSIKMRNLLRQQGKRSF
                     NPGKAAAMAFLTAKDPATIKTIRAGMIGLGYKAQRLGHQLGKSLGLVQGQVAHPPATL
                     GKPPLKAQVIHFINKPMPGGLPKRTSRALLDIEDDTIVPVIRDPRQLRDDSEAVFYFP
                     GCGSERLFSQVGLATQAMLYHVGATTVLPPGYLCCGYPQTSAGDEDKGQRITTDNRVL
                     FHRVANTLNYLDIKTVIVSCGTCMDQLLKYQFEKIFPGCRLLDIHEYLLEKGLKLDGV
                     QGARYMYHEPCHTPMKVHAGLQVANTLMGTRVDLSDRCCGESGTLAVTRPDISTQVRF
                     RKQEEIEKGAARLRGDDSAAPVKILTSCPSCLQGLSRYAHDAGGVEADYIVVEMARHL
                     LGDNWMADYVDKANQGGIERVLL"
BASE COUNT         3529 a         7912 c         8365 g         3435 t
ORIGIN      
        1 aattaaaatc aaaaaagtcg ccgcagccaa gatcagacta gagccatttc tgatcaaaaa
       61 cttcgagaac gacggggtgg tgtatttttt tctgggccga cacacccatt gccggcgccc
      121 aatgcagcca ctgtcacacc attctctgga cgaacccgcg aacccatccg attctcagcg
      181 aatctgtgcc cgcgcaggtt gccactcacc acggcgaata ccgcagatac tacgaaggca
      241 aaatcgcccg atttctgtcg tcaatgccgc cctgccccca ttgcgggaaa aggcaattcg
      301 acagattcat caacaacacc caattcccgc gattcccgag tggacgagac atcccggaag
      361 gcatcaccag cgccgacctg atcaaggtcg acccggacag tacgctcgtg tattacgtga
      421 ctgttgatcc ggtctgagac gcgcctgtca ggcccacgat tggggacttc atgacccgcc
      481 acaccgcctc ccaatccacc gcatccagcg tgttcttcac cagcagcccc aggtcggtct
      541 cgccctccat cagcagacgg cggctgaaga agagggtgtc ggggtcttct tcgcggaggg
      601 cgagggcgag gtagtcgcgg gtggtggcgg agatggtcag gtcgggggtg cggcggtcca
      661 ggacggggcg gaagccgcgg ggggtgaggg tgaagcgcag ggcgaggccg gcgtcggtga
      721 tgcgcagcag gaggcgcttg ccggtgaggg gttcgagggt gtcgcggggg atgaggcggt
      781 cgagggcggc gttcaggccg gcgacgaggg cgagggtcgg gggcagttgc ggcaggcggg
      841 cgccgacgcg ggtgacgagg ctcggcaggg tgaaggcggg cagcttcatg gcaggtccga
      901 tccggtaaag gtcaggcggt gacgaactgt tcgacgccgg cgcggccgtg ccagaagccg
      961 ttgcacggcg cttccggcaa caggggcagg gcttcctgca gggcgtcctg gggcggagtg
     1021 agcccctcca gggcgtcgcg gaagagcgtc acgacctcgg cggtgtgttc gccctggggg
     1081 ctgatgcgca ggatgtcgat gccgtcggca accacggcgg gcagatcgcc gagcagggtg
     1141 tgcacgtggg cggactgggt ctggatgccg ttgaggcaca ggaagggctc gccttcgcgg
     1201 gtcttgaggg ggatgccgtc ggcgaactgc aggcagcgga actcgcaggt gtccttctgc
     1261 aggttgaagt ggcgggcagt gaagcagcgc gcggaatacg ccaggggcag gcggccatgg
     1321 gcgaagacct cggtctccat gtgcagcccc aggtgggcgc gcacggcggc gatctggtcg
     1381 cgggacagtt ccggcggcgc cacccagcgg ctggcgccca ggccggcgaa gaggcccagg
     1441 gtgccggggt tgaagatgtt cagggtcggc ccggcgatcc agtcgggacg cgcaccgtcg
     1501 ccggcggtca ggaggcgtac ggcgcccatg tcgttggctt cgacgcggaa gcggccgttg
     1561 tccacgatgc gacgcagggt cttcaggtcc gattcggatt ccaccagcac ctggctggag
     1621 agcacgacct ccttgccggt cgcggcgagg gtctcggcca cctccagcca gtccgcgagg
     1681 cgcagctcgt ggcgacggga acagacggtt tcgcccaggt agacgatgtc caccggcgat
     1741 tcggcaatct cggcgtagaa ggacagggtg gcggcgcggg gccagtagta cagcaggggg
     1801 ccgagggcga gtttcatcgg ggcgacaggc gggattattt ccagggacgg tagtaggcgc
     1861 cgagggtgtg gctctggcct tcggagacct tgttgagctc ggccatccag gcaggttcga
     1921 cgcggaaagg ccctgcgccg gccagcacgc ggtcgatggc ggcgcgccag acgcgggtca
     1981 cctgggccac gtaggcggga ctgcgctggc ggccttcgat cttgatggca cgcacgccga
     2041 cgcgggccag ctccggcagg agctccaggg tgttgaggct ggtgggttcc tcgatggcgt
     2101 agtaggtctc gccgttcacc tcgaagcggc ccttgcacag ggtcggatag ccggcgcgtt
     2161 cgccgtcggc aaagcggtcg atgaggatgc cgttgaggcg ggtctccatg ccgcgggggg
     2221 tctgctccca gcgcacgtgc ttgcccggcg agcaggcgcc gttgcagttg ggcgattcgc
     2281 cggtggcaaa ggccgacagg gcacagcgcc cctccaccat cacgcacagg ccgccaaagc
     2341 cgaagacctc gatctccacc ggcgtgtggg cgatgacctg ctccacctgg gccagggaca
     2401 gcacccgggg cagcaccgcg cgggcaatgc cgaagtgcgc gtggtagaag ttgatggcct
     2461 cgtagctggt ggccgagccc tgcacggaca gatgcaggcg cagctcggga tgcacgcgcc
     2521 gggcgtaggc catgaggccg ggatcggcga ggatcaccgc gtcgatgccc agttcggcgg
     2581 cccggtccac ggcgccggtc cagcgctgcc aggtatcggt ctggggatag gtgttgaggg
     2641 ccagcaacac cttgcggccg cggtcgtggg cgtaacgcac gccgtcggcc atggccttgg
     2701 ggtcaaagtt gagcccgctg aagttgcggg cgttggtgtc gtccttgaag ccgaggtaga
     2761 cgcagtcggc gccgtggtcc acggcagtct tcaaggcggg caggctgccg gcagggcaga
     2821 ccagttcgat ggcgggcatg ggatcggcgg cagaaatggc gcgggcggac tataggccgg
     2881 gccatctgca gcagccttga caagggtcaa cggccagcgc gcgggccgca ggaatatagg
     2941 ggaatttacg cctttagtcg gatgcgccac gtaggggtgg cagtgcatac ttcagcattc
     3001 actgatacgt ttgaatgacg tccctgccat gactcccaat cgtcgccgct ttctgaccct
     3061 gggtgccgga acgctggcga tggccatgac cggcggagga tgcagttcgg gcagcagcgg
     3121 ccccatcaag ttcgccacca acccctggcc cggttacgac ctgctctatg tcgcccgcga
     3181 caagggcttc ttccaggacg ccggcctgga tgtgaacctc gtggatctca cctccctcgg
     3241 cgcctcacgg cgggccttcg agcgacgcca ggtggacggc tgcggcgcga ccctggtcga
     3301 gatcctgctg gcggccgacc tgtcgggcgt ccgcccgcag attgcggcgg tcgtggacta
     3361 ttccgatggc gccgacgtgg tgatcggtca ggccgccatg tcgtccccca ccagcctcaa
     3421 gggcgcgcgg gttgcgatcg aacccggcac cctgcacatc cagctgctat ccctcgccct
     3481 cgcccgtgca ggcctcacga tccgtgacgt gaccctggta tccatgcccc agaacgtcat
     3541 gcccgccgcc tttgcagaag ggcgcgtgca ggcggcggtg tgctttccgc cggaatccgg
     3601 gcaactgctg gcgaggggcg gcaaggtgct cttccatagt gggcaggtac ccggtgaggt
     3661 ggtggacatc ctggtcttcg acggcgattt cgtggcccgt cgcggcgccg acgtacgcgc
     3721 cttcgtgcgt gcctattacc aggcacagga ctacgcggcc aggcatccgg acgaggccca
     3781 cgccatcatg gcgcgacgcg agaacacctc cctcgccgat ctgcgcgaca ccctcaaggg
     3841 cctgcgctac gtggaccagg cggatcaggc gcgtctgctc gaacccggcg gaccggtcga
     3901 gcgctcgctg atccatctgc agggcgcaat gatcgagctc gcccaactgc gcgaacgcca
     3961 cacgctcaag gacttcctca tggcccgtga gctgctcgcc gcacggggtg ccctggcatg
     4021 agcggcacgc gcaccctgtc cctgcgctgg cgcctgatcg gaccgctgat cggcgtctgg
     4081 gcggtgggtc tcgcgctgct ggtgacggtg ctctacatca acctcctcga ccgcttccgc
     4141 ggcctcgtgg agcagcgcgc cgacaccctc gtcgccgccg tgtattccgc cgccgaaact
     4201 gcccgcgaac cggccgacct gcgccgcttc gtgtccactc tgggcgccgg gcgcgacgtg
     4261 agcagcgtca ccctcctggg cggacgcccg gcacgggtca tcgctgccac cgaccacggg
     4321 ctccacgacc tgggcgtgga cgaactgccc gaccgacccc tcgcggcaca aatctcccgg
     4381 gtgatcctgg agcgcaaggg cgaacgggtg ctcgacgccg agcggcgcag cctggtggtc
     4441 ctcgaaccgg tcctgctgat gggcaccgac ggcgagcacg gccgcctcga agacggcgtg
     4501 ctggccctgc gcctcgatgc caccagcctg tggcgcgccg cccgcgatac cgtgctgcac
     4561 ctgtccgtcg gcctgggggc catcttcagt gccgtcgccc tgggcgccta cctgatgctg
     4621 tcccgttacg tactgcgtcc ggtggatggc atccgcgatg cccttgaccg ccgtgccgcc
     4681 ggcgaggccg acgtcccggt tccgattgcc cgccccgacg aactcggcga actggcccgg
     4741 accctcggcg aactgctcga caccctgcgc atccgcaatc gccagctggt cctcgcccgc
     4801 gatcaccacc tgggcatgct ggagagcttc cctcacctcg cctggcgcgc cggcctcaac
     4861 ggtcaatgcg actggttcaa ccgggcctgg ctgcgcttca cgggccgcgc gctggcccag
     4921 gaggtgggca gcggctggct ggaaaacgtt catccggatg accgggaccg ctgcgcccgc
     4981 gcgttccgtc gccatatcga cgcccgcgaa cccatggccc tggagttccg cctgcgccac
     5041 gccgacggca cctatcactg gatcgccagc catggcgcac ccatgttcga cgacaccggc
     5101 gagttcgtcg gccacgtggg ctcctgctac gacctccagg cctcacgcga caatgcccag
     5161 gccctggcgc gcatgacccg tctgtatcac gccctgtcgg ccaccaacaa ggccatcgtg
     5221 catacccgcg acgccgatgc gctgctgccc caggtgtgcc gcatcgccgt ggagtacggc
     5281 gaactcgagc gggcctggat cggcttccgc agcgcaccgg gccagggctg gcggattgtc
     5341 gccgcccacg gccgcaacgg cccgcttcgc ctcgacgacc ccgcgctgcg ctgccgcctg
     5401 cccgatgccg gtggggccag cccccacatc gacaacgaca tggcgcaggg ctgcggcgcc
     5461 tgcggcgaaa cggacggcgg tgcctgtgcg accttcccca tcgccggcga ccccgagctc
     5521 gacgcgatgc cggacggggc cctcttcctg cgtacatcgg aacgcaattt cttcgacgtc
     5581 cccaaggtcg ccctgctgga ggagatggcc accgacatcg gcttcgccct gcgcaacctg
     5641 cagcgcgacc gcgccctgcg cctggctgcg cgggtattcg agaacaactc cgaaggcatc
     5701 gtggtcatcg ccccggaccg ccgcgtggtg atgatcaacc gggccttcac gaccctcacc
     5761 ggcttcaccc tggacgccgt gcgcaacctg cctctggctc tcttcgatcc ggcccatcac
     5821 ccggccgact tcgaggcaga cctgcgcgac cacgcggccg ccggcgaagt ctggcagggt
     5881 gaagcgtggc tccgccgtgc cgacggcgag gccatgccct gcgccctgac cctcagcgcg
     5941 gtccgctccg acgcgggcgg actgacccat tacgtggcgg tgttctccga catcagccag
     6001 cgcaaggccg acgaggcacg catccgcttc ctggccagtc acgatttcct caccggcctg
     6061 ccctcccggg cagtgctcga accgaccgtc gccgacgcca tcgcggccgc cacccgcagc
     6121 ggacggcgcg ccgccctgtg cttcctcgat ctggatcgct tcaagaacgt caacgacacc
     6181 ctcgggcacc acaccggcga ccgcctgctg gtggaagtgg cccgcaggct gggcgccgtg
     6241 ctgcaggacg gcgaatgcct gttgcgccac ggcggtgatg aattcgtcgt ggtcatgccc
     6301 gaggccggcg accggaatgc ccttgccgcg gcggccgcac gcctcatcgg cgccctcgat
     6361 gcgcccttcg aactggccgg ctgcgaattc agcctgtcgg ccagcatcgg cgtggcggtc
     6421 ttccccgaag acggccacga gcccgacacc ctcctcgacc gggccgacgc cgccatgtac
     6481 cgggccaagg aagccggccg caacgccgtg gagttctttg ccgacggcat ggccgcgccc
     6541 acccgtgaac gcctggccct ggagctcgcc ctgcgccgcg ccctggaacg ccaggaactg
     6601 gcggtccact accagccgct ggtgaaggcc catgacagct ccctgacggg cgccgaagcg
     6661 ctcttgcgct ggcgccatcc ggaactgggc gacatctcgc cggcgcggtt cattcccctg
     6721 gctgaggaaa gcggcctgat cctccccatc ggcacctggg tgctcgacac cgtgtgccgc
     6781 gacctggccg catggcgggc tgcgggacgg accgtcgtcc cggtggcggt gaacctctcc
     6841 gccctgcagt tccggcgcgc ggatttcgtc gccacggtct ccgacatcct gcatcgccac
     6901 gccattccgg ccgagctgct catcctggag gtcaccgaga gcatggtgat gcgcgacgtg
     6961 gagcgggcgg cacaccagct gggcgaactc aaggccctgg ggctgtccat cgccgtggat
     7021 gactttggca ccggctattc cagcctcgcc tatctcaagc gttttcccct cgacaagctc
     7081 aaggtggatc agtccttcgt gcgggagatc aacgccgggc cgggcgaccg ggccatcgtc
     7141 gcggccatcg tcgggctcgg tcacaccctg ggactcagtc tggtggccga aggtgtcgag
     7201 accgaagaga tggcggaaac cctccgcggc ctgggatgcg acctgctcca gggttggctg
     7261 ttcgggcgcg ccgagccggc agccgtcttt tcggagcgcc tcgccggtgc cctgcggtac
     7321 gcctgagcac ccgttcggag cgcagtccgc cggctccccc cctccgacac ccgccgctgg
     7381 cgccacgccc gcacgccagg cccgcgtgcc tccgcccgcg gtgcttctgg acaggcgtcc
     7441 ctgtcgtgcg ctcatggccc ccaatagcgc gcaggagatg acagaaccgt acgacttggg
     7501 cgatcatcgc cctgaaacga gcagtgacat tccctgtccg gcccgaaccg gacctgcgaa
     7561 cttccctgaa acatgaatcc ccttcccgaa tccggccgga tgcacgaccc ttccgcgccc
     7621 ccttccgacg accccgacgg gctcgatgcc ctggaagcgg ccctgtctgc cgatcccgcc
     7681 tgcatcgatg ccgcgcgcaa gcgtgtcacg gcaatccgca caacacccgt cccgaccgcc
     7741 atggtggcgc gggacgtgca ctttccggac gttccggccg tgacgccgcc cgccacgacc
     7801 gcgccgacga caccgcctcc gaagcctggc cgcggcggag ggctgcttgc cgagctgaag
     7861 caggccgtcg ccgaacagaa gcgcgtcgag gcagactcct cgcgggagga agaacgggtt
     7921 cgggaggtca tcggcacggc cctgcgccgc gtctttgaat atctgcacga tctggtcggt
     7981 cagctcaacc agctccgtcc ccccattccc cgcagctata cgctgctggt cacgggcacc
     8041 gtgttcgacg gcctgtcctg gcaggaaggc ttcaccggcc atcgcagcca gccccacacc
     8101 aatggcgacc tgctggagcg cgtgaccctc tcctaccgcc ttggcggagc cggtgacacc
     8161 ctgatcgaac gcacgggcag tggcatcgaa cgtttgcgtc aggccctgtt cgacttcggc
     8221 ttcaagaccg aatgcaccga acgccgcaac gcccgccggg aagtggagtt cgccaccttc
     8281 gcggtgcgca gcgagatttc cgtgcaggtg caatggcggg ccgatttccc gcggcgtcag
     8341 gtcatcgtcg agatgcgcaa cctggaacgc ctgggcacgg tggagttcgc cctgcccgcc
     8401 ttcgccctca atcccgagtt cctcgacgag ttcggccgca tgatgctggg ccagcccaac
     8461 cgcttccgcg aactgctgcc acgctgagca cggcgttcag catcaaaaaa agggcgccct
     8521 cgggcgccct ttcaactggc ggtcgggacg gcgcgcgcag gcgcgcgtcc caccgggatt
     8581 cagcggtccc gatcgaaggt acgaccctcg cgacgcggac ggctgtccgc gcggccggca
     8641 ggacgctcgc caaagcgggc gccaccgaag ccgccacggg catcggaacg cggagccggc
     8701 tggccgaagc tgcgacgcgg gccgtcgtga cgggcatcgc ccgccgcgcg gtgcgcgtgg
     8761 ccgccttcgc ggctgtcccg ggcggggcga tccccttcga aacggggggc gccgaaaccg
     8821 ccttcgcgcg gacgccggtc gccgaagcgc gggccgttgc cgaagctgcg cccgtcacgg
     8881 cgcgggccgg ccgggcgacg ttcttcggtg ggcaggctgg cgcggggctc cagaccgggc
     8941 agggtgtgca cggccatggt ctggcgcgtg tagcgctcga tggcgcgcac caggcgcacg
     9001 tcacgccgct ccgccaggct gatggcgacg ccggtgtcgc cgccccggcc ggtgcggccg
     9061 atgcggtgca cgtagtcggc cgccaccttg ggcaggtcgt agttgatgac gtggctgatg
     9121 cggcgcacgt cgatgccgcg ggcggccacg tcggtggcga tgagcacgcg cagatgaccg
     9181 cgacgcagct ggtcgagggt gcgggtgcgc atgcgctggt tcatgtcgcc gtgcagggcg
     9241 gcaacggaat ggccctgggc ttccaggctc agggtcaggg cgtcggcgtc gcgcttggtg
     9301 gcggtgaaca cgatggcctg atccacggcc acgtcgcgca ggatgccgtc gagcagacga
     9361 tgcttgtggc cgatgtcgtc ggcgaccagc aggcgctgct cgatgttgtc gtggcgggtc
     9421 tgggcggtgg cgatctcgat gcgctcggga tccttgagga ggcgctgcat catgcgcgcc
     9481 acctggccgt cgagggtggc ggagaagaac agggtctggc gttccttggg cagggcggcg
     9541 cagatcgcct cgatgtcgtc cacgaagccc atgtcgagca tgcggtcggc ctcgtcgagg
     9601 atcaggatct cgagacgacc aaaatcgatg cggccacggt tcatctggtc catcaggcgg
     9661 ccgggggtgg ccaccaggat ctcataaggc gcggagagga gcttgttctg caccgggtag
     9721 ggcatgccgc cgacgacact gacggccttg gcgtagcgca ggttcttgcc gtaacgcttg
     9781 gtggcatcgg tgacctgcac agccagctcg cgggtggggg tgagcaccag cacgcggggg
     9841 ccgcgggcct tgatcgtggg ctcggcggtc aggcgctgca gggagggcag catgaaggcg
     9901 gcggtcttgc cggagccggt gcggctggac acgaccagat ccttgccggc cagggcggcc
     9961 gggatggccg ccagctggac ggaggtgggt tcggtgtagc cggcttcgag cacggcctgg
    10021 gcgatgggct cggacagacc gagactggag aaatccatgg agtagctttc cttgcaggag
    10081 gcgcggatgc ggcggccgcg ggaaaaaacg gcaacgggcc tcggcgctcg gagcgcaggg
    10141 gcttgcgggg agtgggactt tccgcgcgaa cgggcggggg aagggactcc ggggctgcct
    10201 gacgggcata tcggcactaa ccggtcagga caagctcacg gcgaatcggg agccttgcgg
    10261 ggggtcaggg acgatgtgac tcggtgcaac cgctttgcac cgggacgcga actatagcga
    10321 taatgcggcg gaaccacaag gaacttgttg catcgcagca gccgttccag gcagccccct
    10381 ttcgacacga ggattcctgc atgcgtttcg acaacagccg cgagagcgac aacatcgaag
    10441 accgccgcgg cgacggcggc ggaggtggcc tgcccatcgg cggacgtggc atcggcctgg
    10501 gcaccgtggt gctggccctg gtggccatgt atttcggcgt ggatccccgc gtggtcctga
    10561 acctggccga acaggcgccg gcccccagcg cgcccgcgca gcaccgcgcc ggccccccgg
    10621 ccaacgaccc ggaaaccgtg ttcgtgcgcc acgtgctggc cgagaccgag gacacctggc
    10681 agaccctctt ccgccaaaac ggccgggaat acgtggatcc gaagctggtg ctgttcagcg
    10741 gcgccacccg caccgcctgc ggcacgggtc agaccgcgat gggcccgttc tactgtccgg
    10801 cagaccagaa ggtctacatc gacctggcct tctaccagga actgaaatcc cgcttccacg
    10861 cgccgggcga gttcgcccag gcctacgtga tcgcccacga ggtgggccac cacgtgcaga
    10921 acctgctggg catctcagcc aaggtgcagg cggcgcggga gcgcgccggc gagcgggaag
    10981 ccaacgccct gtcggtgcgc ctggagctgc aggccgactg cctggccggc gtgtgggcca
    11041 agaacgccga cgcggcccgt cacatcatcg aggccggcga ggtggatcag gccctgcgcg
    11101 ccgcctccgc catcggcgac gacacgctgc agaagcaggc ccagggctac gccgtgcccg
    11161 acagtttcac ccacggcagc tcggagcaac gcacccgctg gttcaagcgc ggcatggagg
    11221 ccggcgacct ggcggcgtgc aacacctttc aggccaagac gctctgagcg cggcgccggc
    11281 gctcacaccg gccggcccgc cgggaccacg tcctgcacct cgaggctgac ccggaaacac
    11341 gcaccgccgc ccgcctgggc gacaagctgg atgtcgccgc ccatacgccg ggcgagctgc
    11401 cgagcaaccg ccagaccgat cccggcgcct tccacgcgcc cggcgttgct caggcgctgg
    11461 tagggctgga acagcgcggc cacttccgcg ggcggaatgc ccggaccgtt gtccgcgatc
    11521 tcgaacccat gcgcgccacc ggggagggtc cgcagggtga cgcgggcgtc cagcgtgtgg
    11581 cgggagaact tgacggcgtt ggacaggagc tggcgcacca ccccgcgcag cagcaccggg
    11641 tcgcccgtca cctgcggtcc gccggcacag tccagacgga ccgcatggcc ctggccgtgg
    11701 cgcgccatca ggtcgggcca cagtgcggtg gccagggcag cgaggttcac cggcgcccgt
    11761 cgcggcggag gggccgcgct gccggccagc ccctccagcc ccacgagcgc agcgcggatc
    11821 cgccggctgg cttcggcgag gcggtccagc gcagcaggca ccttggccgg ggcgtggacg
    11881 agggcgtcgc ggagcaggtc cacctgatcg tccaggctga acacgggacg gcgcaggtcc
    11941 cgcgccacgg ccccggtcac ggcctccatg cgggcgtggg cccctgcgag ggtctcgaac
    12001 tgttcggcga ggctcgcctc acgggcctgg caggcctcca gggcgcgccg caggcgccgg
    12061 gtcatgaacg cgctgatggc cagcaggatg acgagggccg ccagcggcac ggtgaggtag
    12121 agcgcaaacc tgcggaccgg cagctccggc atgtagcgcg tgtcgagcca gcggttgcgg
    12181 gcggtggcga tgtcggcggg cgacagggcc tcgagggcac ggccgaggat cgcggccagt
    12241 tccggcgcgg ccggctggac gcccaggtac agctcgctat cgccgtccgg cacgtggccg
    12301 gcgatgtaga gcttgccgag gaagtgggac tggatcaggc ggttgatcgc gtagagattg
    12361 ccgatggccg catccgcgga ccctgcggcc acccggcgca gggcatcttc ctgggtgggc
    12421 acctccacca gctggatcgt cgggtaggcg ctgcggatgc gttcgcgcag gaagtggctt
    12481 tccagcaggg ccagcttgcg tccggcgaat tcgcccagat cccacacctg ctggaagtcg
    12541 ctgtgggtca cgatggccgt cggggtggtc aggtagggcc cgacgaagag cagcttttcg
    12601 cgacgctccg gcgtgctggc gacacccacc agaacgtcca gctcgttgcg cagggccttg
    12661 tccagcaccg cggaccaggt gcccgccact tcatccaccg acaagccgac cttgtcgcgc
    12721 agcagtcgga acagctcgat ggaatagccc tccgcctggc ggtgcaggtt cgtatagctg
    12781 aggggataga aggcctggtc gtagccgacg cggatcggac catgggcctg gagccaggcc
    12841 tgctcggcgg ccgtcaggtg aaaggcctct ccagggatgc cggtaccggt cggcaggtag
    12901 cggcggagca gcgcgtggcc ctcggcgtcg ctgacgaagg ccatggcttt gcgcaggatg
    12961 cccgccagca gggtctggtc gcgcggcacc gccatcgcca tggcggacag atccgcgtcg
    13021 aaacgctgac gcacctgcag gttcgccagg gacaggcgct ccatgtagta gtgggcgaca
    13081 gccagcaggc cgacataggc atccgcttcg ccggtggcaa ccgcctgcag cgccgacgcc
    13141 gcgtcgtcca cgtccaccag ccgggcgccg ggccgggtgc gaagaaggac ctcgcgggac
    13201 gcatggcccc gttcgatggc gatgcggacg ccccgcagat cggccggcag gccggtgggg
    13261 ccgccgctgc tgcgggtcac cagcaccgcc ggcgaatgca tgtagccccc ggtgaagcgg
    13321 tagcgcttgc ggcgcgattc ggtcggggtg agggtcggga tgaggtcgat ggcgcccgaa
    13381 tcgagggcgt gcagcaccgc cgggaattcg gggaagcgca ccatccggaa ctgcaggccg
    13441 gtacgacggc cgaggagttc gaggtaatcc gcgttggggc cggcgtagcg gtcacccgcc
    13501 tgccaggcat agaagggttg aaaatccggt ccgttgcctg cccgcaggac gggatgcgcg
    13561 tcgagccagg cccgctcctc ggcgctcagg gctgcgcgga aacgcgtgac cgtgtcgccg
    13621 ggatgggcct cgtcttgcgc cacagccggc acaacgaggc cgaccagcat gaccagcagc
    13681 aggagcagcc gcccgcaacc ggcgcacagc cagggaggcg gacggcacga cacgaggcgc
    13741 atgacgaagt ctttccggag gaggttccga acgttcgcag gatatgcgct ttccggagcc
    13801 ctgttccacg cggaatggaa cgatctgtct cgggctccgg gtgccccgga cggggcgcgt
    13861 ggatcagccg gcgaggcgcg cgaaggcgtc gacgacttcg ggcggcgcct gcaccagttc
    13921 gatcagcacc ccttcgccgc cgatggggaa ctcctcattg cccttgggat gcaggaaggt
    13981 gatgtcaaag cccgctgcgc ccttgcggat gccaccgggg gcaaagcgca cgccgttggc
    14041 gctgagccat tccacggcct tgggcaggtc gtcgatccac agccccacgt ggttcagggg
    14101 cgtggtgtgc acggcgggct tcttctcggg atcgaggggc tgcatcagat ccacctccac
    14161 cttgaagggg ccggcgccca tggcggtgat gtcctcgtcc acgttttccc gctcggactt
    14221 gaagttgccg gtgacttcca ggccgaacat gtccacccac agggtgcgca gccggtcctt
    14281 ggacgggccg ccgatggcga tctgctggac gccgaggacc ttgaaaggtc ggttcatggt
    14341 tttctccctc tatcgttgta tcggtgtttg gaggggggat tctacgggcg ggcgggttcg
    14401 ggacggaagg ggagcgcgcc tgcggcacgc gctcagggcg tatcgctccc cgccggacgt
    14461 gccacgaccc ggtcggcttc cggcaccaga ctcaccgtgt aatggccgcc gtcggcccgg
    14521 tggagcgcca gcagatcgta agtccggagc atgtccagca ggccggaatg cccgaaattt
    14581 gccggcgaga agccggggtc ggtgcgcttg aggtactgcc cgagcgcgct caggtgaacc
    14641 tgaccttcgg acgaatttcc agccagcagg gcaaccgcgt ccacaacgaa gcgcggacgc
    14701 cgcttgatca tcccgccgcg ggccgcctcc cccttggtcg ctgcacccgc gggctgctcg
    14761 ggacccgtgc cgttccccgc ctccgtgtcg gccgccggag cccattcgaa gaactggtcg
    14821 ctggcattgc gcagggcctc cggcgtcttg gcctcaccga cgatgcacac ggtggcgccc
    14881 cgctcccgca gcttgcggca cagccccgca aaatccgagt cgctggtcac caggcagaat
    14941 ttgtcggcgc ggtcgtcaaa gaggatctcc agcgcatcca gggcgagggc gatgtccgac
    15001 gtgttcttgc cggccgcata ggtgtattgc aggcagggcg tgaaggcctg acggaccaga
    15061 acctcctgcc aggtccgggc gagggtgctg tggttgccgt agccccgccg cagcacgacg
    15121 cggccaaact gggcagcgac ccgcagggcg tactcgacga tggggggctg ggtgttgtcg
    15181 cagtcggcca gcacggcgac gcgggtttcg gcctgagagg catgcgggac cacggtatgg
    15241 ttcttccgga gaggcgggcg ggccgccggg gctgccactg tcgcacgccg gccacgcctg
    15301 cgtcatcccc gcaatcggtc agggcagacg cagggccgcc cggagacgat cacatgccgg
    15361 tcatggggcg ccgcgatact ggcccgatgg agactccaca caggtccgac cttgccggca
    15421 cgacggccct cgtcaccggc gccagcagcg gcatcggccg tgtcaccgcc cgcgaactcg
    15481 cctgccgggg cgccaccgtg gttctggcct gccgcagcga ggcgcgcacc cagcccgtgc
    15541 tggcggagat cgcgcgcctg accggccggg ccgatgcagc cgtctggctg cccctcgacc
    15601 tggcggactt cgcttcggtg cgcgcctgcg ccgaaggctt cctgcgcctc gaacgtcccc
    15661 ttcatctgct ggtgaacaac gccgggctgg ccggcgcgcg gggtctgacc cgtgacggct
    15721 tcgagatggc gttcggcgtc aatcacctgg gccatttcct gctgacccgt tgcctggagg
    15781 accgcctgat cacctcggcg ccggctcgcg tggtgaccgt cgccagccgc gcccaccggg
    15841 gcgccggcgg tctggactgg gcggccctgc gccggccgac ccgcagcctc acgggcatcc
    15901 gggaatacgc tgcgtccaag ctcgccaaca tcctgttcag cgctgcactg gcgcgccgcc
    15961 tggccgatcg tggcgtcggc gcctacgccg tgcatccggg cgtggtggat accgaggtat
    16021 ggcgcaccgt gccgggcgcc ttgcgtccct tgctcaggct gcgcggcctg ctcacccccg
    16081 aggacggtgc acgcaccagc ctccactgct gcgtcgacgc cctaccgggc gagagcggtc
    16141 tgtacttcgc ccggtccagc ccggtgacgc cgtcccgacg cgcccgcgac ggggcgttgg
    16201 cggacgaact gtggcagcgc agtgaggcgt gggtcgccag ttgaaggctg tccctccggc
    16261 gcgatgctca ggcgccgaac agacgcgccg ccgcagtggg gttggacgga aagtagaagt
    16321 gtacatagga cgccgtcagg cggccgacgc ggtagatcgc ctcgccctcc cgcccggccg
    16381 gcgtgtgggc gcgggcgagc ggctccagcg gcgtctcgct gcgggaatag tggaaggtgt
    16441 gcccggtgag gcgaccttcc ggcaggagga cgatctgggt gccgagggcg gccaggcggt
    16501 cctgcatgcg ggtatgaccg ggcagcaggc cggcgccggg atgggtgacg ccctcccggt
    16561 cggtaatgga cgcaaacagg ctcatcatgc cgccgcactc cgccaggatc ggcttgccgg
    16621 cggccttgtg ggcggccaga tcggcgtgcc aggcgtcatg ggccgccagg gtggcggcgt
    16681 ggagctcagg atagccgccg ggcagccaca gggcgtcgca gggcggcagg ctctcgccgg
    16741 caaggggcga gaagaagcgc agctcggcgc ccagggcgcg cagcaggtcc agattggccg
    16801 ggtagatgaa gccgaaggcg gcatcccggg cgatggcgac cgtgcgcccg gcgaggagcg
    16861 gcggcacgtc ggccgggggc ataccggcga agctgacggg cggcggcaga tcggccgcgc
    16921 cggtacgggc cagatggtct gccagccggt cgagacgcgc gcccaggtcg gcaatctcgg
    16981 ccgcctggag gaggcccaga tgacgctccg gcagggccgc atccgcatcc tggggcaggg
    17041 cgccgtacca ggcgaagctg ccgcccaggc tgtcccgcag caggtcggcg tggccggggc
    17101 tgcccacgtg gttggccagg acaccggaga acggcagacc gggctcccag gtggcgaggc
    17161 catgcaccag ggcgccgaag gtttgagcca tggcgcgggc gtcgatgacg gccatcaccg
    17221 ggatgccgaa atagcgggcg atgtcggcgc cgctgggcgt gccgtcgaag agccccatga
    17281 cgccttcgac gaggatcaga tccgcgtcct gcgccgccgc atggagacgc cacgccgcgt
    17341 cggccgagcc gcacatgccc aggtccaggt tatgcaccgg gtggccgctg gcgaccgcgt
    17401 ggatctgcgg atcgaggaaa tcgggcccgc acttgaagac ccgcacccgg cgcccctggc
    17461 gcgcatgcag ccgggccagc gccgcggtga cggtggtctt gccctggccc gaggccggcg
    17521 cagcgacgaa cagcgccggg cagctggcgt cgggaaagcg cgggttcatg cgccgccctc
    17581 cggattgaac caggccatcc ggccctgcag gctgacgacg ctgcctacca gggtcagggc
    17641 cggcggcgtg atgccggcac gcttcacggc ctccggcagg gtcgccagat cggcagtgac
    17701 cacgcgctgg tccgggctgg tgccatggcg caccacggca gcgggcgtgg tcggtgccag
    17761 gccgtgggcg atgagctggc tgcagatgtc ggtgagccgc gtgatgccca tgtagaacac
    17821 cagggtctgc ttcggccgcg ccagcatgtc ccagtccaga tcgacggtgc cgtccttgag
    17881 gtgaccggtc acgaagacgc aggactgggc gtggtcccga tgggtcagcg gaatgccggc
    17941 ataggcgccg atgcccgatg ccgcggtgat gcccggcacc acctcgaagg gaatgccggc
    18001 ctcgaccagc tcctccacct cctcgccgcc gcgcccgaag atgaagggat cgccaccctt
    18061 gaggcgcacg acccggcgcc cttcccgcgc gaggctcacc agcagcgcgt tgatgccttc
    18121 ctggggcaga ctgtgattgg acgccttctt gcccacgtag tagcgggccg tgccggccgg
    18181 cacgcaggcc aggacttcct tgctcaccag gttgtcgtag accagggcgt cggcctccag
    18241 cagcaggcgc gcggcgcgca gggtcaggag ctctgcatcg ccgggaccgg cgcccaccag
    18301 atagacccag ccgggccggg cggcgccacg ggacggggga agcgtggaat cggacatgga
    18361 ggctctccgg atcaatcgtg cgggcggacg gactcaggtg gcgggcttgc gccacagggc
    18421 cagacgcacc ggcgcatcgg gaacgaaacg cagacgaccg gcgacgcatt cgccccggga
    18481 ctgggacagc tcctgccagg cttcggcggg cacctgggcc gcaaacgtca ccagatcggc
    18541 gcgggcctga tcgtcctcgg cactcaccac caggcggccg ccaggcgcaa ggcgcgccca
    18601 ggccatggcg agccactcgg gcaggccggc gctgccgcgg atgaagacgg cctccggtgc
    18661 aggccaggcc gccgcgtgga tcggcatcgc cgacgggatc gcggccaggt tttcaccgac
    18721 cccgacgcgg gccagggaca gaccgagcag ggtgggatcg acgccgacgg cgcggacacg
    18781 ggcggtcggc acggcgcggg cccattccag ggcgacgctg gcctcgccat cactgatcgc
    18841 ccagcccgtc tcccaggcgg ccggctgcag ccaggcaagc gcgaggacgc gcactgccag
    18901 gggcagcgcg gccccggcgt catcccccag tgccgcggcg gagaggccgg gcagctcggt
    18961 gaatacgccg gccgacgggc cggtcgcgac gatcagcacc gcgtggggat ccaggggctc
    19021 gcgcagttcg accagctccg acgccagcca ggcggtggcc cgcggtgccg tgccgttgaa
    19081 ctcgcacacc cacacgcggg caccgggaaa gccgctctcc agcagcaggc gggccaccgc
    19141 ggcggggcgc tcggcgtcgg cgaccggcac cgccagcagg cggtgggcgc gcagctggcc
    19201 gcgcagggcc gccagcggtg cacggcgcag gtccaccagg gtgacgatct cggccgacag
    19261 gcccagcgcc gcgcacgccg cctgtacgcg cccgacgccg ggcagcacgc gcacgtgggc
    19321 cggtcccagg tcggccagca gttcggcggc cggtccatcg ccggcgccgt cgccaacgca
    19381 caccagcacg gcccccgcgt gccccgcgtt acgcacgtcc agatgcgccg tctcggcgcc
    19441 gcacaggcgc tggggtgctg acagggagag cccgctcagg cggtcgaggg cctcggcagt
    19501 gccgaggatc acgcgggccc cggcgagcgc ctcccgcgcc gcggcggaca gggccggcgg
    19561 caggtccagc cccaggctca cgacggtaag gggcgggggc tcggccggtt cggggatggc
    19621 gtccgcaaag gacggggcga gcgcctcggc cgcagagtcc atcagcgcag cgtcggccgc
    19681 ctcgccatcg gagaccgcgg catcgccatc ggtgtcgcta ccgccgcgac cgcggcggcg
    19741 tttgcgcttg cggcgcggcc cgccggactc gtcgccggct tcgggcacgg caccttctgc
    19801 cgtgatcggc gcggtgggat cgtccgatgc cgccgcttcg tcgggagcgt cagcagccgg
    19861 ttcaggccta ctttctgggt ccgcggcggc gggctcgcgc ggcacgacgg gcagggcctc
    19921 gtccgccacc acagccccat cggagcggac ggctggcgac tccagaacca gttcggcctg
    19981 gggggcggcg aggcgtgccg ccggcagttc cgggggcgtg tcgggaagac tgtcgggctc
    20041 gggcgggcgg gcgctgccgc ggggcgcacg ccgggacggt gcgcgcgggg atttcgcctt
    20101 gcccgcagca cgcttgcggg gtgtcgcctc ggccagcgtc ggcggggcgt cgccctgagg
    20161 gctggcaggt gcgtcgcctg ccgcgggccc gacgggcttt tcgggggtgt ccggaacgtc
    20221 cgcactcttg cggcgcgaag ccgcacgcgg cgcagcgcgg cggcgcgccg gcggggaagc
    20281 gtcggccgga tcggcggccg ggggcgtgat ctgatcgtcg ttcatgcgnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cggcaagccc cttgcccgca acctcaaggc
    20461 ccgcgtgcag accaccgcgc tcgggcagtt ccccttcgcg agcgtggtgg gctgcatcga
    20521 ctcccgcgcc gcaccggaga tcgtcttcga ccagagcatc ggcgacctct tcgtcgcccg
    20581 cgtggccggc aacacagtca atgaggacat cctgggcagc ctcgagtacg ccgccaaggt
    20641 ggcgggctcg cgggtcatcg tggtcctcgg ccacaccagt tgcggcgcca tcaagggcgc
    20701 ctgcgacgac gtgaagctgg gtaacctgac caccctgctg gccaagttca agccggccgt
    20761 ggcggcggcc accacgccgg gcgaacgcac gtcccacaac cacgccttcg tggacgaggt
    20821 gacggagttg aacgtgaagc aggtggtgca gaagatccgc gccgacagcc ccatcctcaa
    20881 gggcctggag gacgagggca ggatccgcat cgtcggcgcc ctctacgaca ccagctcggg
    20941 caaggtgcgc tggtactgag cgcccgcgcc ggaccccgcc tccggcgcgc ctgagaacga
    21001 agaggcccgc gcgcagcggg cctcttcacg tcacggagcg gaaagccgct tacagcagca
    21061 cgcgctcgat gccaccctgg ttggccttgt ccacgtagtc ggccatccag ttgtcgccga
    21121 ggaggtggcg ggccatctcg acgacgatgt agtccgcctc cacgccgccc gcgtcgtggg
    21181 cgtagcggga caggccctgt aggcagctcg ggcacgaggt gaggatcttc accggcgcgg
    21241 cgctgtcgtc gccccgcagc cgggcggcgc ccttctcgat ctcctcctgc ttgcggaagc
    21301 gcacctgggt cgagatgtcc gggcgtgtca ccgcgagggt gccggactcg ccgcagcagc
    21361 ggtcggacag atcgacccgc gtgcccatca gcgtgttggc cacctggagg ccggcatgga
    21421 ccttcatggg ggtgtggcag ggctcgtggt acatgtagcg ggcgccctgc acgccgtcga
    21481 gcttgaggcc cttctcgagc aggtattcgt ggatgtccag caggcggcag cccgggaaga
    21541 tcttctcgaa ctggtacttc aggagctgat ccatgcaggt gccgcaggac acgatcaccg
    21601 ttttgatgtc cagatagttc agggtgttgg cgacccggtg gaagagcacc cggttgtcgg
    21661 tggtgatgcg ctgacccttg tcctcgtcgc cggcggaggt ctgcggatag ccgcagcaca
    21721 ggtagccggg cggcagcacg gtggtggcgc ccacatggta gagcatggcc tgggtggcca
    21781 gccccacctg gctgaacagg cgttcggaac cgcagccggg gaagtagaac accgcctcgc
    21841 tgtcgtcgcg cagctgcctg ggatcacgga tcaccgggac gatggtgtcg tcctcgatgt
    21901 ccagcagggc gcgggacgtg cgcttgggca ggccgccggg catgggcttg ttgatgaagt
    21961 ggatcacctg ggccttcagc ggcggcttgc cgagggtggc gggcggatgg gcgacctgtc
    22021 cctgcaccag gccgagggac ttgccgagct ggtggccgag gcgctgggcc ttgtagccga
    22081 ggccgatcat gcccgcgcgg atggtcttga tggtggccgg atccttggcg gtgaggaagg
    22141 ccatggccgc cgccttgccc ggattgaagc tgcgcttgcc ctgctggcgc aggaggttgc
    22201 gcatcttgat ggagacatca ccgaagtcga tgtccaccgg gcagggcttt tcgcacttgt
    22261 ggcagacggt gcaatggtcg gccacgtcct cgaactcggc ccagtgggcg agggacacgc
    22321 cgcggcgggt ctgctcctcg tagaggaagg cctcgatcag cagggaggtg ctgaggatct
    22381 tgttgcgcgg gctgtagagc agattggcgc ggggcacgtg ggtggagcac accggcttgc
    22441 acttgccgca gcgcaggcag tccttgatgt cgtgggagat ctcgccgatg gcggtctgct
    22501 ccatgatcag ggactcgtgg cccatcaggt tgaagctggg cgtgtaggcg ttgtccaggt
    22561 tgccggtgag ggcaccgtgg cgcatcagct tgccccggtt gaagcggccc tccggatcca
    22621 cctgctgctt gtaggcccag aagttggcca tctccgcgtc ggtgaggtag tccagcttgg
    22681 tgatgccgat gccgtgctca ccggagatca cgccgtcgag ggcgcgggcc agggccatga
    22741 tgcggtccac cgcccggttg gcggtctgga gcatctcgta gttgtcggag ttgaccggga
    22801 tgttggtgtg cacgttgccg tcgccggcat gcatgtgcag ggccacgaag acgcggccgc
    22861 gcagcacctg cttgtggatc gcgcgcagct gctcgaccac cggcgcgaac atctggccat
    22921 cgaagatttt ttccaggcgg ggcttcagct cgctcttcca ggagacccgc acggagtagt
    22981 cctgcaggcg gtggaacagg cgcggccggg ccgcccggtt ggtgagttca ccggccacca
    23041 cgccgtagga gacgaagcgt ggctcggcct cggcgagggg caggtcgagg ttctcgagca
    23101 gccattccca gcgcaggcgc acggcctcca ggtagttgag ggcgtcgcgg cggcggtcac
    23161 cgaggagctc ctcgcgggag atggtggcat cacccgtgtg cagcggcagc tcgccttcca
    23221 ggaaggcgcg cagggcctcg cacagggcca gcttgttctg gatggacagc tcgatgttga
    23281 tgcgctcgat gccgtcgcag tagtcgccca tgcgcggcag cgggatcacc acgtcctcgt
    23341 t
//
LOCUS       DAOQMM010000053        22725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_758_length_22725_cov_47.171592, whole genome shotgun sequence.
ACCESSION   DAOQMM010000053 DAOQMM010000000
VERSION     DAOQMM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 22725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22725
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_22725_cov_47.171592"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLH85_10000"
     CDS             <1..182
                     /locus_tag="PLH85_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB64939.1"
                     /translation="WDKLPKAGAMVAAVAHKEYAEMGVGALAEKIVSGGVFTDVKSFY
                     DPAAIEAAGAKVWRL"
     gene            299..1420
                     /gene="wecB"
                     /locus_tag="PLH85_10005"
     CDS             299..1420
                     /gene="wecB"
                     /locus_tag="PLH85_10005"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB64940.1"
                     /translation="MSRSLIYLVAGARPNFMKIAPIVRALEARSDRFEYKIIHTGQHY
                     DREMSDVFFDELGIPKPDYHLEAGGGSHATQTAKIVVAFEEICRQVRPDCVLVVGDVN
                     STLACSIVAKKQVIPVAHVEAGLRSGDMRMPEEINRLVTDSISDWFFCTEPSGVENLL
                     REGKVKDVVFHVGHVMVDNLLYQRDKLDASDKSGLTTEAIKAKQPKYGVVTLHRPSNV
                     DEEPTLRGIVGALRQIAEQLPLIFPVHPRTRGNLERFGLDLGPNVLLTKPQSYMEFLN
                     LWKDAALILTDSGGLQEETTALGIPCLTLRENTERPVTADEGSNVLVGPNPQRILEEA
                     AKVLSGAGKQGRRPALWDGKAAERIVAELDRLLEWSTSV"
     gene            1549..2640
                     /locus_tag="PLH85_10010"
     CDS             1549..2640
                     /locus_tag="PLH85_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNB64941.1"
                     /translation="MILAAGQGTRVRPLTKNIPKPMVPILGKPLMEYLIEHLARHDIR
                     QIMVNVAFNHYKIENFFGDGHRWGVEMGYSYEGKRDHGDIVPKPLGSAGGMRRIQDFG
                     GFFDETTLVLCGDAIVDLDITAAIAEHKAKGAIATVVTVEVPLEQVKNYGVVVADEQG
                     RVMSFQEKPKPEEAKSRLASTGIYIFEPAVLDYIPPGVEFDIGSQLFPKLVELGVPFY
                     AQRHAFNWIDIGRLTDYWDVLQRVLRGEVAQMHMPGQEVAPGVWVGLNTSIAWESVQL
                     VGPVYIGSNVRIEPGVSVIGPAWIGHGSHLRSGSRVVRSILFEYTRVAEGMVFTEKIL
                     SPQYCVDRSGEAVYVGDDTCPLRWGDARA"
     gene            complement(2917..3600)
                     /locus_tag="PLH85_10015"
     CDS             complement(2917..3600)
                     /locus_tag="PLH85_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64942.1"
                     /translation="MPKSYYLPTDDSGKIEVLEMLAAKLPAYKDVLYISTEEIEAVQA
                     DAIAMRYILSVFNQMQNNAKQWTAYKNLIRDGGASKAALPVFPSLPDPVPPTVPPGII
                     PRLTSLVTRIKAARNYTDVIGQDLGIVGTVKVVDPATWKPTLDTRIEAGRPYILWSKG
                     KADSLEIWVDRGNDKGFVFLDISTDYRFADNAPMPNTVALWKYKAIYRLRDQQVGQWS
                     NVHAVPVGG"
     gene            4288..>4615
                     /locus_tag="PLH85_10020"
     CDS             4288..>4615
                     /locus_tag="PLH85_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988028.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HNB64943.1"
                     /translation="MKIAIAGTGYVGLSNAILLAQHNEVVALDIVPTKVEQLNRKESP
                     IEDREIEEYLRAKPLNFRATLDKREAYDGAEVVIIATPTDYDTETNFFNTRSIESVVR
                     DVLAINP"
     assembly_gap    4616..4715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4716..5609
                     /locus_tag="PLH85_10025"
     CDS             <4716..5609
                     /locus_tag="PLH85_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB64944.1"
                     /translation="FNTRSIESVVRDVLAINPQALMVIKSTVPVGYTAKLREQFGIDN
                     VIFSPEFLREGRALYDNLYPSRIIVGERSERAQAFADLLRQGAIKQDVQVLLTDSTEA
                     EAIKLFANTYLAMRVAYFNELDSYAAAHGLDTRQIIDGVCLDPRIGSHYNNPSFGYGG
                     YCLPKDTKQLLANYRDVPQNLIHAIVESNTTRKDFIADEIIRRRPRTVGIFRLIMKAG
                     SDNFRASSIQGIMKRIKAKGIEVIVYEPVLKQDEFFNSRVVNDLAAFKAQSDVIIANR
                     RTDALADVGDKVFTRDLFGSD"
     gene            5748..6491
                     /locus_tag="PLH85_10030"
     CDS             5748..6491
                     /locus_tag="PLH85_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNB64945.1"
                     /translation="MKKTLLAYGAAARSLLRADILWHLMWPTILSAALWVGGLAFGWS
                     ALVASAQDGLVALPWLGSWLAGSPGALVVALVLFKIALVVLVLPVIYVTAAFLVAAIA
                     LPLMLETVARTDYADLARRGGGSTWGSVWNALVAVIVFLLLLVASLPLWLIPGAGLVI
                     SLLLTAWLNQRAFRYDALMTHADAYELKALPQQQSAGLFGVGLLGAVLAHVPLLNLFA
                     PAVTGLAFVHYLLAALRDARRTENVIEVV"
     gene            6492..7283
                     /locus_tag="PLH85_10035"
     CDS             6492..7283
                     /locus_tag="PLH85_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNB64946.1"
                     /translation="MSDLREAAPAFGAIIIGDELLSGRREDKHLAKVVELLKARGLRL
                     ASARYVGDERPRLVQTLRESFASGDIVFSFGGIGATPDDHTRQAAAEALGLPLALHPE
                     AEAEIRGRFGDETTPQRLQMGVYPVGSAIIPNPYNRIPGFSIRQHFFVPGFPVMAWPM
                     VEWVLDTRFAHLHHRTDYVERSILVWEALEGKLIDLMETLTADYPDLTLFSLPCFGGD
                     EVRRHVELGMKGSAARVDEAMARIVAEVARRGYRWDAPGADASAA"
     gene            7294..7671
                     /locus_tag="PLH85_10040"
     CDS             7294..7671
                     /locus_tag="PLH85_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64947.1"
                     /translation="MSGGRSGYRDFPGRRWLVVALRAGHMAGVVGVGAALLAEPMVAP
                     EPAFLVLLVAAGAAMALLDAYSNPDWLREPAGWSLFAKFALLGWYMADAAVRLPLFWA
                     ILVFSVLFAHAPASFRHRRPNGR"
     gene            7754..8305
                     /locus_tag="PLH85_10045"
     CDS             7754..8305
                     /locus_tag="PLH85_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64948.1"
                     /translation="MELSDIPVKTAAGHEEIARRDRRLPTRLRTLLILADGKRSVQAL
                     ADATVGFGPVDEPLAHLVTLGLIEPMRKAAPPDPSSAPALPASRAAQPQGVPEKPAHE
                     RPAPRPGQRRSLALARLYLLNAMEQSLRQDDQPIRDCLRAAKTRAELLQAFEVCREVA
                     QEVGVKHIRTIEAQFFEMLPEEG"
     gene            complement(8345..10396)
                     /gene="fusA"
                     /locus_tag="PLH85_10050"
     CDS             complement(8345..10396)
                     /gene="fusA"
                     /locus_tag="PLH85_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB64949.1"
                     /translation="MTPTSVRDIRNLSLLGQSGSGKTTLLEKLLATAGAIGAPGSVEK
                     GDTVSDFDPQEKTFGHSLNTALAHLEWAEHWVNLLDTPGLPDFLGRALASLPAVETAV
                     VVINAAAGVETVTRRIMDAAADKCRMLVVNRIDAPGVDFGALMEHIVASFGKECLPIN
                     LPLAGGTGVVDCFFKPDYDAVPAFSSVREAHDRIVDQVVEVDEDLMALYLEQGESLQP
                     AQLHDPFEQALREGHLIPVCFTSARSGAGVSELLDILGRLLPDPTEGNPPHFLKGEGP
                     AAQPVEATTAADQHVIAHVFQVSNDPFRGKLGLFRIHQGTVTPATQLFVGDARKPFKV
                     GHLLRIQGKTQTEITVGIPGDLCAVARVDEIHRDAVLHDSHDEDHFHLVPPRYPQPVF
                     GLALQPEKHGDEQKLSDALHRLLDEDPCLSVEHDTQAKETVIRGLGEVHLKVVLEQLK
                     TRWNLSLVTRPPAIPYRETIAGTAEARYRHKKQSGGAGQFGEVALRVETLERGAGVVF
                     ADEVKGGVIPGNFMPAVEKGVHQAVIEGVMAGFPLQDLRVVVFDGKHHAVDSNEISFV
                     TAARHAVQEAVQAARPVVLEPLVTLTVRIADEHFGGISAELSGRRGRVTGTDAPQAGV
                     TEITSLVPMAELEGFESRLKSISGGDGSCAIAFSHYEPAPAEVQQRLAREHAGKRGGE
                     Q"
     gene            complement(10490..12205)
                     /locus_tag="PLH85_10055"
     CDS             complement(10490..12205)
                     /locus_tag="PLH85_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNB64950.1"
                     /translation="MDRFLPFLAWRRRLDAAGLRADLLAGVTVALVAIPQALAYAQLA
                     GLPPYIGLYASLLPSIVGALFGSSAQLNTGPVALTSLLTATTLAPLAPPGSQTYLLLA
                     VQLALLSGLLQVIFGALRLAHFADLLSHPVLHGFINACAVLICVAQLPALLGLPIHGQ
                     RPFVEAVVEILTRLPELHLPSAALGGAAVLMLILLRRFAPRWPGILIVVASLTLVSEA
                     IDFAAQGGRVVGTLPEHIVAFDWPGMDWSQMTELLPSAFVLALVSFLEAMSSCKVASA
                     RTGERWDGNQELIGQGLAKLTASVCQAYPVSGSFGRSAFLLAAGARTGLASVVGAVMV
                     LAALLAAPELIARIPRPVLAAVIFVTLSALLSPKPLQEAWQASRDDGLAGTVSFAATL
                     LLAPHIEIGILSGLILSLAFLIYRSMTPRVALLGRHPDGTWRDADRFRLAPPHPRLII
                     LRFDGPLHFVNAATFEDAVLDATRRHPQLQVLLLACAGINDMDASGVDTLRRIHRQFH
                     EAGHTLACCGLKKQVIDVLERTGLWAGLAPHAAYRTEDHALSALLPLVGVAAPDPHAR
                     RGLAW"
     gene            12481..13635
                     /locus_tag="PLH85_10060"
     CDS             12481..13635
                     /locus_tag="PLH85_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HNB64951.1"
                     /translation="MNDFSDTALTPETATLLIVDDVPENLEVLAELLQSRYRVRAAQS
                     GARALQVACTPPRPDLILLDVMMPDMDGFEVAARLRADPATRDIPVIFVTALDGVQEE
                     EHGLELGAVDYISKPLRPSIVLARVNTQLELKRARDHLRDHNEFLEAEVARRMGENQL
                     IQDVSIRALARLAEIRDPEMGYHLQRTQAYVELLARSLRDHPRFADTLTPRTILLLGK
                     SAPLHDIGKVGIPDHILLKPGKLTPDEWEIMKTHAKLGSDAIELAEKDEAQPVAFLTF
                     AKEIARHHHERWDGSGYPDGLRGDEIPLSARLMALADVFDALTTRRVYKAPMSMETAR
                     RIIVEGRGNHFDPDVVDAFLAAFDGFAEIALHYRDEFPEIDAPQPSTARD"
     gene            13723..18093
                     /locus_tag="PLH85_10065"
     CDS             13723..18093
                     /locus_tag="PLH85_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB64952.1"
                     /translation="MVLVILAAGYGLGVMVSADRNGNESRRLEAVARLKQQQLQTWLT
                     ERRADARSLGQDRSMAELVTRVQGGDRVARQWLQDRLVSVGQAYDYATVTLVNSRGEP
                     LVGLPALPDAFAARLRALWQDGHPGEQSVWADLASGQEGLAFIAPVAGAGDVAAVLLA
                     DPSRSLFPTLQTWPSPSVSAETLLARRDGADVEFLNSVRHRQVETGTFRMPLDWTPVP
                     LVMEGMLHPERMGTVLLGVDYRGVPSAGVVLPVAGTDWVLVTKMDRDEWQADARRAVS
                     WIALGCLAAVMVVGAGALLRYQRQELTHSRRVFRAQAEQLRGLRLLDAIAGTSPDAML
                     AWDRDGVIRFCNPAASRLIGVEAESLVGSRGERARASEALQRIQALASAGGAVVEAVH
                     GSEVRVGEGAAARIHYVTVGPLPDADGEGGVFAMARDITSLRREEEALREREELYSAI
                     VNQAADGIVLLDAQSLNFVEFNDAACHGLGYSRDEFAALNLRDVQQVPVRDVSELRVT
                     RLAVGEAVSFSNLHRRKDGSVRQVELNYRSLNIRGHAFLIGVWRDTTDKLEAEERLRK
                     LSMAVEQSPTSVLIADMAGTIEYVNEAFLHSTGYAREEVIGQMAGFNRSGVTPKETTK
                     ALWDALQAGQAWEGEFINRRRDGELRIEHARIAPILRADGSVSHYLGLQEDVTRRKQV
                     EAELAQYRQHLEERVAERTAQLEEAYRDLTGRSAQVAELNAALERRAAELEAARDASD
                     AANRAKSAFLANMSHEIRTPMNAIIGMTHLLRRGVLDAEQQGRLEKVGDAAEHLLSII
                     NDVLDISKIEAGKLSLDVSDFRVEEVIRKACGLVADKAREKGLELVMDVDEPPYVLKG
                     DATRVGQMVLNYLGNAVKFSSSGVVLVTARVEDEGESSARLHVAVRDQGVGISPEALP
                     RLFRPFEQADGSTTRKYGGTGLGLAITGHLARLMDGTTGVESTPGQGSLFRFSVRVDR
                     GDPRPAVPSGLPALSALVVEPVAEARASLARILNVLDVHVRVVDSDAAAQAALAEMAA
                     RGEHPDVVFTGPGRGGPAEAVADPRIRFVRVTAGDDARGQSIQTGGAAATLTKPLGVS
                     AVLETLQALFVHAEGAVAVSAGRSPAELELGRRHAGARILLVEDSPINQEVAVALLEG
                     CGLVVDVAEDGARAVEHMQAHAYALVLMDMQMPVMDGLEATRRIRALPGGASVPVVAM
                     TANAFGEDRARCLAAGMNDHLAKPVDPAQLYAMLLRWLGGAPATGPAATSDAERLREA
                     LEAIPGLDVDYGLKNLRGRVSSFVRLLRKYADGHADDPQRIRAARQAGEHAEAARMAH
                     SLKGVAGMIGVRTVQEAATRLDAALKAGEGEARVAELIDALAVQQAAAVAGIAALGEG
                     EAEAAAEVDPTLIRQVLGQLEALLREDNVAVGRVLREQLAVVRAGLGADAGPFERAVA
                     AFDFARALALLESAQTVQPH"
     gene            18148..19368
                     /locus_tag="PLH85_10070"
     CDS             18148..19368
                     /locus_tag="PLH85_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB64953.1"
                     /translation="MKTSDPAGPGASHWPLVLLASAVLMITMGVRQSLGLYLSPLNTA
                     TGLGVASISFAMAVGQFMWGAAQPVFGAVADRYGPARALFTGAVMLVAGLALTPFVTS
                     ETGLILCLGVLVAAGAGAGSFSILIGALAQRVPPQKRAFASGFINAGGSFGQFLFAPL
                     SQFLITGYGWVVALFGMAAAAALTIPLSWPLRHKVEHPPAESGAETGLKAQLRVALRD
                     RSYWCLHAGFFTCGFHIAFLVTHLPGAVNLCGLPASVSAVSLAVIGLANIVGSLGAGA
                     LGQHYRMKWILAVMYASRAVLVALYLMAPKTEWTFYLFAVGLGVTWLATVPPTAGLVG
                     KLFGLRYLATLFGLTLLSHQIGGFFGAWLGGLALVNFGNYDWMWYADIVLALLAALAN
                     VPIREVSPRRAVAV"
     gene            complement(19414..>22725)
                     /locus_tag="PLH85_10075"
     CDS             complement(19414..>22725)
                     /locus_tag="PLH85_10075"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNB64954.1"
                     /translation="RCGEERGNFLNARYRNAFPAGYREDYDARSAVGDIELMESLAAD
                     GHLAMSLYVPLEAPPGTLRFKVFRDTDPVPLHVSLPLLEHMGVRVLEERPYEIERADG
                     QKVWMHDFGLSVRGADEIDVDRVRGAFQDAFRHILAGDAESDDFNRLVLAVGLTWREV
                     AVLRAYAKYLRQTGFTFSQAYMEQTLAAHPALARQLLELFIARFDPDLGEDRAEHVAS
                     LAAALQADLDEVANLDEDRILRQFLAVIQASLRTNWFRRDGGAPRPCLSFKLDPHAIP
                     GLPEPRPLYEIFVYAPRVEGIHLRGGKVARGGLRWSDRMEDFRTEVLGLMKAQMVKNA
                     VIVPVGSKGGFVVKNPPPASDRDAFLKEGVACYHLFLRGLLDLTDNRVGGAVVPPPDV
                     VRHDGDDPYLVVAADKGTATFSDYANGVSAEYGFWLRDAFASGGSAGYDHKKMGITAR
                     GAWESGKHHFRALGVDIQTQAFTVVGVGDMSGDVFGNGMLLSRQTRLVAAFDHRHIFI
                     DPMPDPATSFAERERLFALPRSSWADYDARLLSPGGAIVPRSAKSVSLSPEARTALGI
                     EADTLTPQELVRAILVAPVDMLYNGGIGTYVKASSQRHAEVGDRANDAVRVDGRDLRC
                     KVVVEGGNLGFTQLGRIEYARAGGRINTDAIDNSAGVDCSDHEVNIKILLNLVIEDGE
                     LTEKQRNALLAGMTDEVALLVLRDNYFQNSILSGIRARGTRMLDEHARYIRHLVARGR
                     LNRDVEFLPPDDEIAARKAACQALTAPELAVLLAYGKMELFDALLASDVPEDPYIRTA
                     LHRYFPAPLRERYPQHIERHPLRREIIATHVVNSMVNRVGPSFAYRLSEETGAPLPDV
                     VRAYIATREVFGFVPLWQRIEALDNRVDHGAQIKLVYEGWRLIQRGTLWFLRHREHLR
                     DLAETLARFAGSVDQLAAGLYDLITPAYRDQLDQGMAVYLEQGVPADLVRHVACLDAL
                     YAALDIVETGAETGRPVELVARVYFALAGCLDLHRLARRIGTLPTDSHWQGLARNALR
                     DDLSTQARGLATDVLRETPDEADPFRLLEHWKARRAFELERCRQIVAEVLAAPAADLA
                     MLSVALRELRGLAMK"
BASE COUNT         3477 a         6858 c         8424 g         3866 t
ORIGIN      
        1 cctgggacaa gctgcccaag gccggcgcca tggtggccgc ggtggcccac aaggaatatg
       61 ccgagatggg tgtgggcgcg ctggccgaga agatcgtgtc cggcggtgtg ttcaccgacg
      121 tgaaaagctt ctatgatccg gctgccatcg aggcggcggg ggccaaggtg tggcggctgt
      181 aagcgcgagg ggccttgcgg ctgtgtaacg tcgcaaggcg acaattattt ctttgtgctg
      241 cctcaggcgg gcgcgcgata atgcgcgccc gcactgtttg tactcgccgg agctttccat
      301 gtctcgctca cttatttacc ttgttgccgg agcccggccg aacttcatga agattgcacc
      361 gatcgttcgc gcgctggagg cgaggtcgga tcggtttgag tacaagatca tccacaccgg
      421 ccagcactat gaccgggaga tgagcgatgt cttcttcgac gagttgggca ttcccaagcc
      481 tgactaccac cttgaagccg gggggggcag ccacgcgacc cagacggcca agatcgtggt
      541 ggccttcgag gaaatctgca ggcaagtccg gccggattgc gtgctggttg ttggcgacgt
      601 caattccacg ctggcgtgct cgattgtcgc aaagaagcag gtgatccccg tggctcacgt
      661 ggaggcgggc ctgcgtagcg gggacatgcg gatgcccgag gaaatcaacc gtctcgtcac
      721 cgacagcatc tcggactggt tcttctgcac cgagccctcg ggtgtcgaaa acctgctgcg
      781 tgagggtaag gtcaaggacg ttgttttcca tgttggtcac gtgatggtcg ataatctctt
      841 gtaccagcgg gacaagctcg atgcgtcgga taaatctggc ttgacgaccg aagccatcaa
      901 ggcgaaacaa cccaagtacg gtgttgtaac ccttcaccgg ccatccaatg tggatgaaga
      961 gccgacgctg cgaggcatcg tcggcgcgct ccgtcagatt gccgaacagc tgcccctgat
     1021 ctttccggtg catcctcgca cccgtggcaa cctggagcgc ttcggcctgg atcttggccc
     1081 gaatgtgctg ctgaccaagc ctcagtcgta tatggagttc ctgaatctgt ggaaggacgc
     1141 ggcgctgatc ctcactgata gtggcggcct tcaggaggaa accactgcct tgggcattcc
     1201 ctgcctgacg ctacgcgaga acactgagcg cccggtgacg gcggacgagg gtagcaacgt
     1261 gcttgtcgga cccaaccccc agcgcattct cgaagaagct gccaaggtgc tctctggcgc
     1321 gggcaaacag gggcgccgtc ccgccttgtg ggacggcaag gccgccgagc gcatcgttgc
     1381 cgaactggat cggttgctgg aatggagtac gtcagtttag tttattgcag atattgggaa
     1441 tttagattat aggaattcag aaactcctat ctttcgtaaa tgttgtttgg aaatagttag
     1501 gctgatattt atggcgctta tatagcggag agtgtattgg caaaaggcat gatcctggcc
     1561 gcaggccagg gcacgcgggt ccgcccgctc acaaagaaca tccccaaacc catggtgccc
     1621 atcctcggta agccgctgat ggaatacctg atcgaacatc tggccaggca cgatattcgc
     1681 cagatcatgg tgaacgtggc gttcaaccat tacaagatcg agaatttttt cggcgacggg
     1741 catcgctggg gtgtggagat ggggtattcc tacgagggca agcgggatca tggcgatatt
     1801 gtgccaaagc ccctcggctc ggccggcggc atgcggcgaa ttcaggactt cggtggtttc
     1861 ttcgatgaga ccacactggt gttatgtggt gacgcgattg tcgatctgga cattactgct
     1921 gcgattgcag agcacaaggc caaaggggcg attgccacgg tggtgacagt cgaggtgccg
     1981 cttgagcagg tgaaaaatta cggtgtggta gtggcggacg agcagggacg agtgatgtcc
     2041 tttcaggaga agcccaagcc ggaagaggcc aaatcgcgtt tggcgagtac cggtatctac
     2101 atctttgagc cggcggtgct tgactatatt ccccctggcg tggagttcga tattggcagc
     2161 cagttgttcc cgaagctggt ggagttgggg gtgccgttct atgcgcagcg gcatgcgttc
     2221 aactggatcg atatcggcag gctgacggat tattgggatg tgttgcaacg ggtgctgcgt
     2281 ggtgaagtgg ctcagatgca catgccgggc caggaggtgg cgccaggggt gtgggttggg
     2341 ttgaatacgt cgattgcgtg ggaaagcgtg cagctcgttg ggccggttta catcgggtcc
     2401 aatgtgcgta tcgagcccgg cgtgtccgtc atcgggccag cgtggatcgg gcacggtagc
     2461 catctccgct cgggttcgcg cgtcgtgcgg agcattcttt ttgagtacac ccgcgtggcg
     2521 gagggcatgg tcttcacgga gaagatcctg tcaccccagt attgtgtcga ccgcagtggt
     2581 gaagcggtat atgtcggtga cgatacctgc ccgttgcgtt ggggggatgc ccgtgcgtga
     2641 gcgtgatgca gtgctgcatg cttcagagcc ttgcaggcgg cacaggcttg ttgcgaggcg
     2701 ttgaagggtt catctatggg atctgttttt tggggattcc ggaacgcttt aggcggtttg
     2761 cgttgcggct cggtgatcta ttccaagtgg cggatcgaca atgcgccgct gcgctgtttt
     2821 ttgcgcacag gcggagaaag ccgctcagtt aggggagagc gagccgggca tgtgtgccgc
     2881 ggctcgcggc gtgttgcctg agatgattcg ccggatttat ccgccgacgg gcaccgcgtg
     2941 gacgttgctc cactggccga cctgttgatc cctgagccgg tagatggcct tgtatttcca
     3001 gagggcgacg gtgttgggca tgggggcgtt gtcggcaaag cggtagtcgg tgctgatgtc
     3061 gaggaacacg aagcccttgt cgttgccccg gtccacccag atttcgaggg agtcggcctt
     3121 gcccttggac cacaggatgt agggccggcc ggcttcgatg cgggtgtcga gggtgggttt
     3181 ccaggtggcg ggatcgacga ccttgacggt gccgacgatg ccgagatcct ggccgatcac
     3241 gtcggtgtag ttgcgggcgg ccttgatgcg agtaaccagg gaggtgaggc ggggaatgat
     3301 gcccggcgga acggtgggcg gcaccgggtc tggcaggctg gggaagacgg ggagcgccgc
     3361 cttgctggcg ccgccgtccc ggatgaggtt cttgtaggcc gtccattgct tggcgttgtt
     3421 ctgcatctgg ttgaagacgc tgaggatgta gcgcatggcg atggcgtcgg cctggacggc
     3481 ttcgatttct tcggtgctga tgtagagcac gtctttgtag gccggtagtt tggcggcaag
     3541 catctccagc acttcgatct tgccgctgtc gtcggtcggg aggtagtagc tcttgggcat
     3601 gatgtctctc cagtctcaat aggggtagat caggtcatcg ggcaggtctg gcacggtgtg
     3661 ccgcacgccc ggaaacgtgt gatgaagcgt ggggatgtgt tgtgtcaggt cgggcacctc
     3721 cggaatgtgt tgaaaagatg gttgcggaac cccacggaca cgatgtggga gcgttggcag
     3781 aatgtttgag atgtgtgaaa cgatccgaag ggtgtcatgc aggccctgcg ggatgtgcgg
     3841 cgggaaatat gtgatgtgca aatgatcctg gtggacgtgc catgggatcc ggggacggtg
     3901 aggcaggaaa ggaagatggc ggagaacatg gtgaaggctc acgctggacg attgttggtg
     3961 aacaaatgta cagtgttttt actatagacc atttgcgtag atgtgtagaa atatttgatg
     4021 cgtttggggt gtgtggatga attttgggta tgtggattgc cgggtccgtt gcccaaatgc
     4081 atttctgcca acccgggcgc agtgccatca ggtgccttcg gccggatgga gaagggcttt
     4141 tgggggctgc gcgtgccggt ggctcggcgt tctggggatg gaggtgagat gagttccgcg
     4201 gagcgcgatt tcggtcgagc cgttctcaag acggtaacga cttcgactta aaatgttcat
     4261 tcctccccta tggaatccgc tccctccatg aagatcgcca tcgccggcac cggctacgtc
     4321 ggactgtcca acgccatcct gctggcccag cacaacgaag tcgttgccct ggacatcgtt
     4381 cccaccaagg tggagcagct caatcgcaag gagtcgccca ttgaggatcg ggagatcgag
     4441 gaatacctgc gcgccaagcc gctcaacttc cgggcgacgt tggacaagcg ggaggcctac
     4501 gacggggccg aggtggtcat cattgccacg cccaccgact acgataccga gaccaacttc
     4561 ttcaacaccc gctccatcga atccgtggtg cgggacgttc tggcgatcaa cccccnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcaa cacccgctcc atcgaatccg
     4741 tggtgcggga cgttctggcg atcaaccccc aggccctgat ggtcatcaaa tccacggtgc
     4801 ccgtgggcta caccgccaag ctgcgggagc agttcgggat cgacaatgtg atcttctcgc
     4861 cggagttcct gcgggagggg cgtgcgctgt acgacaacct gtacccgtcg cgcatcatcg
     4921 tgggcgagcg ttcggagcgg gcgcaggctt ttgccgatct gctccgccag ggcgcgatca
     4981 agcaggatgt gcaggtgctg ctgaccgaca gcaccgaggc ggaggccatc aagctcttcg
     5041 ccaacaccta cctggcgatg cgggtggcct acttcaacga gctggacagc tatgcggcgg
     5101 cccacgggct ggatacccgc cagatcatcg acggggtgtg cctggatccg cgcatcggca
     5161 gccactacaa caacccgagc ttcggctacg gcggctactg tctgcccaag gacaccaagc
     5221 agctgctggc caattaccgg gatgtgcccc agaacctgat ccacgccatt gtcgagtcga
     5281 acaccacgcg caaggacttc atcgccgatg agatcatccg tcgccggccg cgcacggtgg
     5341 ggatcttccg gctgatcatg aaggccggtt cggacaattt ccgggcgtcg agcatccagg
     5401 gcatcatgaa gcggatcaag gcgaagggca tcgaggtcat cgtctatgag ccggtgctca
     5461 agcaggacga gttcttcaac tcccgcgtgg tgaacgatct ggcggccttc aaggcccagt
     5521 cggacgtgat catcgccaac cggcgcacgg acgcgctggc ggatgtgggg gacaaggtct
     5581 tcacgcggga tctgttcggc agcgactgat ctcccgtgga ggcgccgggc gggaggcggt
     5641 gcctgcccga tctccggccg cgcgacctcg atggctggta tcccatgtcc gcttcgggga
     5701 caatgccgcc cgtggcgggc cggcacggcc gcgaagcgga gcgagcaatg aagaagaccc
     5761 ttctggctta tggggcggcg gcgcgttcgt tgctgcgggc cgacatcctg tggcatctca
     5821 tgtggccgac gatcctgtcg gcggccctgt gggtgggcgg gctggccttt gggtggagtg
     5881 cgctggtggc gtcggcccag gacgggctgg tggcgctgcc ctggctcggc agctggctgg
     5941 caggttcgcc gggggcgctg gtggtggcgc tggtgctgtt caaaatcgcg ctggtggtgc
     6001 tggtcctgcc ggtgatctat gtgacggcgg cctttctggt ggcggcgatc gcgttgcccc
     6061 tgatgctgga gacggtggcc cgcacggatt acgcggacct ggcgcgccgt ggcggcggca
     6121 gtacctgggg cagcgtgtgg aatgccctgg tcgcggtgat cgtcttcctg ctcctgctgg
     6181 tggcgagcct gcccttgtgg ctgatccccg gcgccgggct ggtgatctcc ctgctcctca
     6241 ccgcgtggct caaccagcgg gccttccgtt acgatgcgct gatgacccac gcggatgcct
     6301 acgagctcaa ggccctgccg cagcagcagt ccgccgggct gttcggcgtg ggcctgctgg
     6361 gggcggtgct ggcccacgtg ccgctgctca acctgtttgc gccggccgtg acggggctgg
     6421 ccttcgtcca ttacctgctg gcggcgctgc gggatgcgcg tcgcaccgaa aacgtgatcg
     6481 aggtggtgtg aatgtcggat cttcgagaag ccgcgccagc ctttggcgcg atcatcattg
     6541 gcgacgagct gctctccggg cgtcgcgagg acaagcatct ggccaaggtg gtggaactgc
     6601 tcaaggcccg cgggctgcgg ctggcctcgg cgcgctacgt gggggacgag cggccgcggc
     6661 tggtgcagac cctgcgcgag agcttcgcca gcggcgacat cgtgttcagt ttcgggggca
     6721 tcggcgccac gccggacgat cacacccgcc aggcggcggc cgaggcgctg gggctgcccc
     6781 tggccctgca tccggaggcc gaggcggaga tccgcgggcg cttcggcgac gagaccacgc
     6841 cccagcgtct gcagatgggc gtgtatccgg tgggcagcgc gatcattccc aatccctaca
     6901 accggattcc gggcttctcg atccgccagc acttcttcgt gcctggcttt ccggtgatgg
     6961 cctggccgat ggtggagtgg gtgctggata cccgcttcgc gcacctgcat caccgcacgg
     7021 actacgtgga gcgttcgatc ctggtgtggg aggcgctgga agggaagctc atcgacctga
     7081 tggagacgct gaccgccgac tacccggacc tgaccctgtt cagcctgccg tgctttggcg
     7141 gtgacgaggt gcgccggcat gtggagctgg gcatgaaggg ttcggccgcc cgcgtggacg
     7201 aggcgatggc acgcatcgtg gccgaagtgg cgcggcgggg ttaccggtgg gacgctccgg
     7261 gcgcggacgc gtcggcggcg taagggtgcg tcggtgagcg gcggacggtc cggctatcgg
     7321 gattttccgg ggcggcgctg gttggtggtg gccctgcggg ccgggcacat ggcgggcgtg
     7381 gtgggcgttg gcgcggccct gctggccgaa cccatggtgg cgccggagcc ggcctttctg
     7441 gtgttgctgg tggcggccgg ggcggccatg gcgctgctgg acgcctattc caatccggac
     7501 tggctgcggg agccggccgg ctggtcgctg ttcgccaagt ttgcgctgtt gggctggtac
     7561 atggccgatg cggcagtgcg tttgcccctg ttctgggcca tcctggtgtt ctccgtgctg
     7621 ttcgcccacg cgccggcgag cttccggcac cgtcgcccca acgggcgctg aacggggcgc
     7681 ggcgtgacgg atgtcacgct gctgcagtct gcgctgcgta tcctcccgga cgctttaaag
     7741 tggaggatgc gcgatggagt tgtcggatat tcccgtcaag acggcggcgg gacacgagga
     7801 gatcgcgcgt cgcgaccgac gtctgccgac ccgcctgcgg accctgctga tcctcgccga
     7861 tggcaagcgg tcggtgcagg cgctggcgga cgccaccgtc ggcttcgggc cggtggatga
     7921 gcccctggcc caccttgtga cgctcggcct gatcgagccg atgcgcaaag cggcacctcc
     7981 ggatccatcg tcggcccccg cgctgccggc cagtcgcgct gcgcaacccc agggcgtgcc
     8041 cgagaagccg gcgcacgagc gcccggcgcc gcgtcccggc cagcgccgtt ccctggcgct
     8101 ggcgcggctc tacctgctca atgccatgga gcagagcctg cgtcaggacg accagccgat
     8161 ccgcgactgc ctgcgcgccg ccaagacccg cgcggagctg ttgcaggcct ttgaggtgtg
     8221 ccgggaggtg gcccaggagg tgggcgtcaa gcacatccgg accatcgagg cccagttctt
     8281 cgagatgctg cctgaggagg gctgagacgg gggccgggcc gaggcccccg tccgcacggg
     8341 cggcttactg ctcgccgccg cgcttgccgg cgtgttcccg ggcgaggcgc tgctgcacct
     8401 cggcaggggc cggctcgtag tggctgaagg cgatggcgca gctgccgtcg ccgccgctga
     8461 tggacttgag gcggctctcg aagccttcca gctctgccat gggcaccaga ctggtgatct
     8521 cggtgacgcc ggcctggggc gcatcggtgc cggtgacgcg cccgcgccgg ccggagagtt
     8581 cggcgctgat gccgccgaag tgctcgtcgg cgatgcgcac ggtgagggtc acgaggggtt
     8641 ccagcaccac cgggcgggcg gcctggacgg cttcctgcac ggcgtgacgg gcggcggtga
     8701 cgaaggagat ctcgttggaa tcgacggcgt ggtgcttgcc gtcgaagacc accacgcgca
     8761 gatcctgcag cgggaagccg gccatcacgc cttcgatcac ggcctggtgc acgcccttct
     8821 cgacggcggg catgaagttg ccggggatca cgccgccctt cacttcatcg gcgaagacga
     8881 cgccggcgcc gcgctccagg gtttcgacac gcagggccac ttcaccgaac tgaccggcgc
     8941 cgccgctctg cttcttgtga cggtagcggg cttcggccgt gccggcgatg gtctcgcggt
     9001 agggaatggc gggcgggcgg gtgaccaggc tgaggttcca gcgggtcttg agctgttcca
     9061 gcaccacctt gaggtgtacc tcaccgaggc cgcggatgac ggtctccttg gcctgggtgt
     9121 cgtgttcgac ggagaggcag gggtcttcgt cgagcaggcg gtggagggcg tcggagagct
     9181 tctgctcgtc gccgtgcttt tccggttgca gggcgaggcc gaagacgggc tgcgggtagc
     9241 gcggcgggac caggtggaag tggtcttcgt cgtgggagtc gtggagcacc gcgtcccggt
     9301 ggatctcgtc cacccgcgcc acggcgcaca ggtcgccggg gatgccgacc gtgatctcgg
     9361 tctgggtctt gccctggatg cgcagcaggt ggccgacctt gaagggcttg cgggcgtcgc
     9421 cgacgaagag ctgggtggcc ggcgtgacgg tgccctggtg gatgcggaac aggcccagct
     9481 tgccgcggaa ggggtcgttg ctgacctgga agacgtgggc gatgacgtgc tggtcggcgg
     9541 cggtggtggc ttcgacgggc tgggcggcgg ggccttcgcc cttgaggaag tggggcggat
     9601 tgccttcggt ggggtcgggc agcaggcggc cgaggatgtc gaggagttcg ctgacgccgg
     9661 cgccgctgcg ggcagaggtg aagcacacgg ggatcaggtg gccttcccgc agggcctgct
     9721 cgaagggatc gtggagctgg gcgggttgga gggattcgcc ttgttccagg tagagggcca
     9781 tgaggtcttc gtccacctcg accacctggt ccacgatgcg gtcgtgggct tcgcgcacgg
     9841 aggagaaggc cggcacggcg tcgtagtcgg gcttgaagaa gcaatccacc acgcccgtgc
     9901 cgcccgccag gggcaggttg atgggcaggc attccttgcc gaagctggcg acgatgtgct
     9961 ccatcagggc accgaaatcc acgcccggcg cgtcgatgcg gttgaccacc agcatgcggc
    10021 acttgtcggc ggcggcgtcc atgatgcggc gggtgacggt ctcgacaccg gccgcggcgt
    10081 tgatgacgac gaccgcggtc tcaaccgccg gcagggaggc cagggcgcgc ccgaggaagt
    10141 ccggcaggcc cggcgtgtcg aggaggttga cccagtgctc ggcccattcc aggtgggcca
    10201 gggcggtgtt gagggagtgc ccgaatgtct tttcctgcgg gtcgaaatcg ctgaccgtgt
    10261 cgcccttttc gaccgagccg ggggcgccaa tggcaccggc ggtggcgagg agcttttcca
    10321 gcagggtggt cttgccgctg ccggactggc cgaggaggct gaggttgcgg atgtcgcgga
    10381 ccgaagtggg tgtcatgggg cgctcccttg tggtgtgttc tgaagtgtcg gctgcatgac
    10441 agtatcggcc cgtcaccggc ctgcaacaac gatctggatc aaatgccctt caccatgcaa
    10501 ggccgcgccg ggcgtgggga tcgggcgcgg cgacgccgac caggggcagc agcgcggaga
    10561 gggcgtggtc ttcggtgcga taggcggcgt ggggcgccag cccggcccac agaccggtgc
    10621 gttcgagcac gtcgatgacc tgcttcttga gcccgcagca ggccagggtg tgacccgcct
    10681 cgtggaactg gcggtggatg cggcgcaggg tgtctacgcc gctggcgtcc atgtcgttga
    10741 tgccggcgca ggcgaggagc agcacctgca gctgggggtg gcggcgggtg gcgtcgagga
    10801 cggcgtcctc gaaggtggcg gcgttgacga agtgcagggg gccgtcgaaa cgcaggatga
    10861 tcaggcgcgg gtggggcggt gccaggcgga agcggtcggc gtcgcgccag gtgccgtccg
    10921 ggtggcggcc gaggagggcg acccgcgggg tcatgctgcg gtagatcagg aaggcgaggg
    10981 acaggatcag gccggacagg atgccgatct cgatgtgggg cgccagcagc agggtggcgg
    11041 cgaagctgac ggtaccggcg aggccgtcgt cacggctggc ctgccaggct tcctgcaggg
    11101 gtttgggcga gagcagggcg gaaagcgtga cgaagatgac cgccgccagc accggacgcg
    11161 gaatgcgggc gatgagctcg ggtgcggcga ggagggccgc cagcaccatc accgcgccga
    11221 ccacggaggc cagcccggtg cgggcccccg ccgccagcag gaaggcggag cggccaaagg
    11281 agccgctcac cgggtaggcc tggcagacgg aggcggtgag tttggcgagg ccctggccga
    11341 tgagctcctg gttgccgtcc cagcgttcac cggtgcgggc cgaggcgacc ttgcagctgg
    11401 acatggcctc caggaagctc accagggcca gcacgaaggc ggagggcagc agctcggtca
    11461 tctggctcca gtccatgccg ggccagtcga aggcgacgat gtgctcgggc agcgtgccga
    11521 ccacgcgccc accctgggcg gcgaagtcga tggcttcgga gacgagggtg aggctcgcca
    11581 cgacgatcag gatgcccggc cagcggggcg cgaagcgccg cagcaggatc agcatcagca
    11641 ccgccgcgcc gcccagggcg gcggagggca ggtgcagctc gggcaggcgg gtgaggatct
    11701 cgacgacggc ctcgacgaag gggcgctggc cgtggatggg caggcccagc agcgccggca
    11761 gctgggccac gcagatgagc accgcgcagg cgttgatgaa gccgtgcagc accggatggg
    11821 acagcaggtc ggcaaagtgg gccaggcgca gggcgccgaa gatcacctgg agcaggcccg
    11881 acaggagggc cagctgcacc gccagcagca ggtaggtctg ggagccgggc ggggccagcg
    11941 gcgccagggt ggtggcggtg agcaggctgg tgagggccac cggtccggtg ttgagctggg
    12001 cggacgagcc gaaaagcgca ccgacgatgc tcggcagcag ggaggcgtag agcccgatgt
    12061 agggcggcag gccggcgagc tgggcgtagg cgagggcctg gggaatcgcc accagggcga
    12121 ccgtcacgcc ggccagcagg tcggcccgca ggccggcggc gtcgaggcgc cggcgccagg
    12181 cgaggaaggg caggaagcgg tccatgcgga cgtctcgggc gcggggacgg attctgggca
    12241 gaacgaagcg cgcattgtgt cacggcggcg cgacgaccgg gctaaagtcg ctcgcaggat
    12301 gaaccgtaat gcagcagacg gaaagccggt cggggatgct ccggatcgac tatcgtgaaa
    12361 ttgattcctg ccgccggggg ccggtccggc ggcgtgacct gggtcggttg ggggcgggca
    12421 tcgccactcc tccggcggct tccggcctcc tctcagtctt atggcgggga ccccggcttc
    12481 atgaacgatt tttccgatac cgccctgacg cccgaaaccg cgacgctgct gatcgtcgac
    12541 gatgttccgg agaacctgga agtcctcgcg gagctgctgc aatcccgcta tcgggtgcgt
    12601 gccgcccagt cgggcgcgcg cgcgctgcag gtggcgtgca cgccaccgcg gccggacctg
    12661 atcctgctcg acgtgatgat gccggacatg gatggcttcg aggtcgcagc gcggctgcgc
    12721 gccgatccgg ccacccgcga cattccggtg atcttcgtga ccgccctcga tggcgtgcag
    12781 gaggaggagc acgggctcga gctgggggcc gtggactaca tcagcaaacc cctgcgcccg
    12841 tccatcgtgc tggcgcgggt caacacccag ctcgaactca aacgcgcccg cgaccatcta
    12901 cgcgaccaca acgaattcct ggaggccgag gtggcccggc ggatgggcga gaaccagctc
    12961 atccaggatg tgagcatccg cgccctggcc cgcctggcgg agatccgcga tccggagatg
    13021 ggctaccacc tgcagcgtac ccaggcgtac gtggagttgc tggcccgcag cctgcgcgac
    13081 catccgcgct ttgccgacac gctgacgccc cgcaccatcc tgctgctggg caagtcggcg
    13141 ccgctgcacg acatcggcaa ggtggggatt ccggaccaca tcctgctcaa gccgggcaag
    13201 ctcaccccgg acgagtggga aatcatgaag acccacgcca agcttggcag cgatgccatc
    13261 gaactggccg agaaggacga agcccagccg gtggccttcc tcaccttcgc caaggagatc
    13321 gcccgccatc accacgagcg ctgggacggc agcggttatc cggacggcct ccgcggcgac
    13381 gagattcccc tgtcggcacg tctgatggcc ctggcggacg tgttcgacgc gctcaccacg
    13441 cgacgggtgt acaaggcgcc catgagcatg gagacggccc ggcggatcat cgtcgaaggg
    13501 cgcggcaatc atttcgatcc ggacgtggtg gacgccttcc tggcggcctt cgacggattt
    13561 gcggagattg cgctgcacta ccgggacgag ttcccagaga tcgacgcccc gcaaccatcc
    13621 actgcccggg actgagtgcc catgtctttg ccggacacga gggcggcggg gcggagcgag
    13681 cggcaggccc gagtccgggc cctcacccgg accttcgtgg tcgtggtcct ggtcatcttg
    13741 gcggccggat acggtctcgg cgtgatggtc agcgcggacc gcaacgggaa cgagagccgc
    13801 cgtctcgaag ccgtggcgcg cctgaagcaa cagcagctgc agacctggct gacggagcgc
    13861 cgtgcggatg cccgttcgct gggccaggat cgcagcatgg ccgagctggt gacgcgcgtg
    13921 cagggcgggg acagggtggc gcgtcagtgg ttgcaggacc gcctcgtcag tgtggggcag
    13981 gcctacgact acgcaacggt gacgctggtc aacagtcgcg gtgaacccct ggtcggcttg
    14041 cctgccctgc ccgatgcgtt tgcggcccgt ctgcgggccc tgtggcagga cgggcatccg
    14101 ggcgagcagt cggtgtgggc tgacctggcg tccgggcagg agggcctggc cttcatcgcg
    14161 ccggtcgccg gtgcgggcga tgtggcggcg gtactgctgg cggatccgtc ccgcagtctg
    14221 ttcccgacac ttcagacctg gccttcgccc agcgtgagcg cggagaccct gctggcgcga
    14281 cgggatggcg cggatgtgga gttcctgaac tccgtgcgcc atcgccaggt ggagaccggt
    14341 acgttccgga tgccactgga ctggacgccg gttccgctgg tgatggaggg catgctccac
    14401 ccggagcgga tgggcacggt gctgctcggc gtggattacc gcggcgtgcc ctcggccggc
    14461 gtggtgctgc cggtggccgg aacagactgg gtcctggtca ccaagatgga ccgggacgaa
    14521 tggcaggcgg acgcgcgccg ggcggtgtcg tggatcgccc tgggctgtct ggcggccgtg
    14581 atggtcgtcg gggcgggggc gctgctgcgc taccagcgcc aggaactgac ccattcccgg
    14641 cgcgtctttc gggcccaggc cgaacagctg cgcggtctgc gcctgctgga cgccatcgcc
    14701 gggacctctc ccgacgccat gctggcctgg gatcgggacg gcgtgatccg cttctgcaat
    14761 ccggcggcga gccgtctgat cggggtggag gcggaatccc tggtgggcag tcgcggcgaa
    14821 cgggcccgcg cgtcggaggc cctgcagcgc atccaggcgc tggcgagcgc gggcggggcg
    14881 gtcgtcgagg cggttcacgg ttcggaggtc cgggtcgggg aaggggctgc ggcccgcatc
    14941 cattacgtca cggtgggccc gctgcccgac gcggacggcg aaggcggtgt cttcgccatg
    15001 gcgcgggaca tcaccagcct gcgccgcgag gaagaagcgc tgcgggagcg ggaagagctc
    15061 tacagcgcca tcgtgaatca ggctgcggac ggcatcgtgc tgctggacgc gcagagcctg
    15121 aatttcgtgg aattcaacga cgccgcctgc cacggactcg gttacagccg cgacgaattc
    15181 gccgccctga acctgcgcga cgtgcagcag gtgccggtgc gggatgtgtc ggagctgcgc
    15241 gtgacccggc tggcggtggg cgaggcggtg agcttttcca acctgcaccg gcgcaaggac
    15301 ggcagcgtgc gtcaggtgga gctcaattac cgctcgctga acatccgcgg tcatgccttc
    15361 ctgatcggcg tgtggcgcga caccaccgac aagctggagg ccgaagagcg tctgcgcaag
    15421 ctgtccatgg cggtggagca gagccccacc agcgtgttga tcgccgacat ggcgggcacc
    15481 atcgagtacg tgaacgaggc cttcctgcac agcacggggt atgcccggga agaggtcatc
    15541 gggcagatgg cgggtttcaa ccgatcaggc gtgacaccca aggagaccac caaggccttg
    15601 tgggacgctc ttcaggcggg gcaggcctgg gagggggaat tcatcaaccg ccggcgcgac
    15661 ggcgaactgc gcatcgagca tgcccgcatc gcacccatcc tgcgggcgga cggcagcgtc
    15721 agccactatc tgggcctgca ggaggatgtc acccggcgca agcaggtgga ggccgagctg
    15781 gcccagtatc gccagcatct ggaggagcgc gtcgccgaac gcaccgccca gctcgaggag
    15841 gcgtaccggg acctgaccgg ccgctcggcc caggtggcgg agctgaatgc cgccctggag
    15901 cgccgggctg ccgaactgga ggccgcgcgg gatgcgtcgg acgcggccaa ccgcgcgaag
    15961 agcgccttcc tggccaacat gagccacgag atccgtacgc ccatgaatgc catcatcggg
    16021 atgacccacc tgctgcggcg cggggtgctc gacgcggagc agcagggacg cctggaaaag
    16081 gtgggcgatg cggcggagca cctcctctcc atcatcaacg acgtcctcga catctcgaag
    16141 atcgaggccg gcaagctgtc cctggacgtg tccgattttc gtgtcgagga ggtgatccgc
    16201 aaggcctgcg gactggtggc cgacaaggcc cgggagaagg gcctggagct ggtgatggac
    16261 gtggatgaac cgccctatgt gctgaagggc gacgccaccc gggtcgggca gatggtgctc
    16321 aattacctgg gcaacgcggt gaagttctcg tcgtccggtg tggtcctggt gacggcgcgg
    16381 gtggaggacg aaggcgaatc ctcggcgcgg ctccacgtgg cggtgcgcga tcagggcgtc
    16441 ggcatttcac cggaggccct gccgcgcctc ttccggccct tcgagcaggc ggatggctcc
    16501 accacccgga aatacggcgg caccggcctc ggtctggcca tcaccggtca tctggcgcgt
    16561 ctcatggacg gcaccaccgg ggtcgagagc acgccgggcc agggcagcct cttccggttc
    16621 tcggtgcgcg tcgatcgggg cgatccccgg cctgccgtgc catcgggtct gccggccctc
    16681 tccgccctgg tggtggagcc ggtggccgag gcgcgggcat ctctggcgcg catcctgaac
    16741 gtgctggatg tgcacgtccg cgtggtggat tcggatgccg ccgcgcaggc ggcgctggca
    16801 gaaatggcgg cccgcggcga gcatcccgac gtggtcttta ccggtccggg gcgcggtggg
    16861 cccgccgagg ccgtggccga cccccgcatc cggtttgtgc gtgtcacggc gggcgacgat
    16921 gcccgggggc aatcgatcca gaccggcggt gcggcggcga ctttgaccaa gccgctgggg
    16981 gtgtcggcgg tgctggagac cttgcaggcc cttttcgttc acgccgaagg ggccgtggcg
    17041 gtcagcgccg ggcgcagtcc ggccgagctg gagctgggcc ggcggcacgc cggcgcccgc
    17101 atcctgctgg tggaggacag cccgatcaac caggaagtgg cggtggcctt gctggagggc
    17161 tgcggtctgg ttgtcgacgt cgccgaagac ggggcccgtg cggtggagca catgcaggcg
    17221 cacgcctacg ccctggtcct gatggacatg cagatgccgg tgatggacgg tctggaggcg
    17281 acccgccgca tccgtgccct gcccggcggg gcttcggtgc cggtcgtggc catgaccgcc
    17341 aacgccttcg gcgaggaccg ggcgcgctgc ctggcggcgg gcatgaacga tcatctggcc
    17401 aagccggtgg acccggccca actctacgcc atgctgctgc gctggctggg cggcgcgccg
    17461 gcgacggggc ccgcggcgac ttccgacgcg gagcggctgc gggaagcgct ggaagcgatt
    17521 cccgggctgg acgtggatta cggactcaag aacctgcgcg gccgggtgag cagcttcgtg
    17581 cgtctgctac gcaaatacgc cgatggtcat gcggacgacc cgcagcgcat tcgcgcggcc
    17641 cggcaggccg gcgagcacgc ggaggcggcg cgcatggccc attccctgaa aggcgtggcc
    17701 ggcatgatcg gggtgcgaac cgtgcaggag gctgcgaccc gtctggatgc ggccctgaag
    17761 gccggggagg gcgaggcacg ggtggccgaa ctgatcgacg cgctggcggt gcagcaggcg
    17821 gccgcggtgg cggggatcgc ggccctcggc gagggggagg cggaagccgc cgccgaggtc
    17881 gatccgacgc tgatccggca ggtgctggga caactggagg ccctgttgcg ggaggacaac
    17941 gtggcagtcg gccgcgtgct gcgcgaacag ctggcggtcg tgcgggccgg gctgggggcc
    18001 gatgccgggc cgttcgagcg ggccgtggcg gccttcgact tcgcccgtgc cctggcgctg
    18061 ctcgagtcgg cccagaccgt tcagccgcat tgaccggggc gacgttccgg gcgatgctcg
    18121 ggctccccaa ccgaaccggt cttcataatg aaaacatccg accctgccgg cccgggcgcc
    18181 agtcactggc ccctggtgct gctggcctcc gcggtgctga tgatcaccat gggcgtacgc
    18241 cagtccctcg gcctgtatct ttcacccctc aacacggcca ccgggctggg cgtcgccagc
    18301 atcagcttcg ccatggccgt cgggcaattc atgtggggcg cggcccagcc ggtgttcggc
    18361 gcggtggcgg atcgttatgg tccggcgcgg gccctgttca cgggcgccgt catgctggtg
    18421 gccgggctgg cgctgacgcc tttcgtgacg agcgaaaccg gcctgatcct gtgtctcggc
    18481 gtgctcgtgg cggccggcgc gggggcgggc agcttctcca tcctgatcgg tgcactggcc
    18541 cagcgggtgc cgccccagaa gcgcgccttc gcgtcgggct tcatcaacgc cggcggttcc
    18601 ttcgggcagt tcctgttcgc gccgctttcc cagtttctga ttaccggcta cggctgggtg
    18661 gtggccctgt tcggcatggc ggcagcggcg gccctgacga ttcccctgtc gtggccgctg
    18721 cgccacaagg tggagcatcc gcccgccgag tcgggcgccg agacgggcct caaggcgcag
    18781 ctgcgggtgg ccctgcggga ccgcagctac tggtgcctgc acgcgggctt cttcacctgc
    18841 ggcttccaca tcgccttcct ggtgacgcac ctgcccggcg cggtgaatct gtgcggtctg
    18901 ccggcgagcg tgtcggcggt gtcgctggcg gtgatcgggc tggccaacat cgtgggcagc
    18961 ctgggggccg gcgcgctggg ccagcactac cggatgaaat ggatcctggc ggtgatgtac
    19021 gcgtcccgcg cggtgctggt ggccctctat ctgatggcgc ccaagacgga gtggaccttc
    19081 tacctttttg cggtggggct gggcgtcacc tggctggcga cggtgccgcc cacggccggg
    19141 ctggtgggca agctgttcgg tctgcgttat ctggcgacgc tgttcggcct gaccctgctg
    19201 tcccaccaga tcggtggctt cttcggcgcc tggctgggcg ggctggcgct ggtgaatttc
    19261 ggcaactacg actggatgtg gtacgcggac atcgtgctgg ccttgctggc cgcgctggcc
    19321 aacgtgccga tccgtgaggt gtcgccgcgc cgcgcggtgg cggtctgagg cgccttcgcc
    19381 ggggatgccc cggcgaaggc tgaacccggc ggcctacttc atcgccaggc cgcgcagctc
    19441 ccgcagggcc acggacagca tcgccaggtc ggccgcgggg gcggccagga cttcggcgac
    19501 gatctgtcgg cagcgctcca gttcgaaggc gcggcgggcc ttccagtgtt ccagcaggcg
    19561 gaacggatcg gcttcgtccg gcgtctcgcg caggacatcg gtggcgaggc cgcgggcctg
    19621 ggtggacagg tcgtcccgca gggcgttgcg ggcgagaccc tgccagtggc tgtcggtggg
    19681 cagggtgccg atgcgccggg cgaggcggtg caggtccaga cagccggcga gggcgaagta
    19741 cacccgcgcc acaagttcca ccggccgccc ggtctcggcg ccggtctcga cgatgtccag
    19801 ggccgcgtag agggcgtcca ggcaggccac gtggcggacc agatcggccg gtacgccctg
    19861 ctccaggtac accgccatgc cctggtcgag ctgatcccgg taggcgggtg tgatcaggtc
    19921 gtagaggccg gcggccagct gatccacgct gccggcgaag cgggcgaggg tctcggccag
    19981 gtcgcgcagg tgttcccggt ggcgcaggaa ccacagggtg ccgcgctgga tcaggcgcca
    20041 gccttcatac accagcttga tctgggcacc gtgatcgacc cggttgtcga gggcctcgat
    20101 gcgttgccac aggggcacga agccgaagac ctcgcgggtg gcgatgtagg cgcgcaccac
    20161 gtcgggcagc ggcgcgccgg tctcttcgct gaggcggtag gcgaagctcg gcccgacccg
    20221 gttgaccatg ctgttcacca catgggtggc gatgatctcc cggcgcaggg ggtggcgctc
    20281 gatgtgctgc gggtagcgtt cgcgcagggg cgccgggaag tagcggtgca gggccgtgcg
    20341 gatgtagggg tcttccggca catcggaggc gagcagggcg tcgaacagct ccatcttgcc
    20401 gtaggccagc agcacggcca gctccggcgc ggtgagggcc tggcaggcgg ccttgcgggc
    20461 ggcgatctcg tcgtccggcg gaaggaattc cacgtcccga ttgaggcggc cgcgagccac
    20521 caggtggcgg atgtagcggg cgtgctcgtc gagcatgcgc gtgccgcggg cgcggatgcc
    20581 ggacaggatg ctgttctgga agtagttgtc gcgcaggacc agcagggcga cctcgtcggt
    20641 catcccggcg agcagggcgt tgcgctgctt ttcggtgagt tcgccgtcct cgatgaccag
    20701 gttgaggagg atcttgatgt tcacctcgtg gtcggagcag tccaccccgg cggaattgtc
    20761 gatggcgtcg gtgttgatgc gcccgccggc acgggcgtac tcgatgcggc cgagctgggt
    20821 gaagcccagg ttgccgcctt ccaccaccac cttgcagcgc aggtcgcgcc cgtccacgcg
    20881 gaccgcgtcg ttggcgcggt cgcccacctc ggcgtggcgc tggctgctgg ccttgacata
    20941 ggtgccgatg ccgccgttgt agagcatgtc cacgggggcg acgaggatgg cgcggaccag
    21001 ttcctggggt gtcagggtgt cggcttcgat gcccagcgcg gtgcgggctt ccggcgacag
    21061 gctgacggat ttggcgctgc gcggcacgat ggcgccgccc ggcgagagca ggcgggcgtc
    21121 gtagtcggcc cacgaggagc ggggcagggc gaacaggcgt tcccgctcgg caaagctggt
    21181 ggccggatcg ggcatggggt cgatgaagat gtggcggtgg tcgaaggccg ccaccaggcg
    21241 cgtctggcgc gacaggagca tgccattgcc gaagacgtcg ccggacatgt cgccaacgcc
    21301 gaccacggtg aaggcctggg tctggatgtc cacgcccagg gcgcggaagt ggtgcttgcc
    21361 ggattcccat gcgccgcggg cagtgatgcc catcttcttg tggtcgtagc cggcggaacc
    21421 gcccgaggcg aaggcgtccc gcagccagaa gccgtattcg gcggagacac cgttggcgta
    21481 gtcggagaag gtggcggtgc ccttgtcggc ggcaaccacc aggtaggggt cgtcgccgtc
    21541 gtggcggacc acgtccggcg ggggcaccac ggcgccgccg acccggttgt cggtgagatc
    21601 cagcaggccg cgcaggaaga ggtggtaaca ggccacgccc tccttcagga aggcgtcccg
    21661 gtcgctggcc ggcggcggat tcttgaccac gaagccgccc ttggagccga ccggcacgat
    21721 caccgcgttc ttgaccatct gggccttcat cagccccagc acctcggtgc ggaagtcctc
    21781 catgcggtcg gaccagcgca gcccgccacg ggcgaccttg ccgccgcgca ggtggatgcc
    21841 ttcgacgcgc ggcgcataca cgaagatctc gtacaggggg cgcggttcgg gcaggccggg
    21901 gatggcgtgg gggtcgagct tgaaggacag gcaggggcgc ggtgcgccgc cgtcgcggcg
    21961 gaaccagttg gtgcgcaggc tggcctggat gacggcaagg aactggcgca ggatgcggtc
    22021 ctcgtccagg ttggccactt catccagatc ggcctgcagg gcggcggcca gacttgcgac
    22081 atgttcggcg cggtcctcgc ccaggtccgg gtcgaagcgg gcgatgaaca gctccagcaa
    22141 ctgccgggcg agggccgggt gggcggcgag ggtctgttcc atgtaggcct ggctgaaggt
    22201 gaagccggtc tggcgcaggt atttggcgta ggcgcgcagc accgccacct cgcgccaggt
    22261 gaggcccacg gccagcacca ggcggttgaa gtcgtcgctt tctgcgtcgc ctgccagaat
    22321 gtggcggaag gcgtcctgga aggcgccgcg cacccggtcc acgtcgatct cgtcggcgcc
    22381 gcggacggac aggccgaagt cgtgcatcca cactttctgg ccgtcggccc gctcgatctc
    22441 atagggacgc tcctccagca cgcgcacgcc catgtgctcc agcaggggca gggagacatg
    22501 caggggcacc gggtcggtgt cgcggaagac cttgaagcgc agggtgccgg ggggcgcctc
    22561 caggggcacg tagaggctca tggccaggtg gccgtcggcg gcgagggatt ccatcagctc
    22621 gatgtcgccc accgcgctgc gggcgtcgta gtcttcccgg taaccggccg ggaaggcgtt
    22681 gcggtagcgg gcgttgagga agttgccgcg ctcctcgccg cagcg
//
LOCUS       DAOQMM010000054        22519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_768_length_22519_cov_56.542512, whole genome shotgun sequence.
ACCESSION   DAOQMM010000054 DAOQMM010000000
VERSION     DAOQMM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 22519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22519
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_768_length_22519_cov_56.542512"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLH85_10080"
     CDS             complement(<1..344)
                     /locus_tag="PLH85_10080"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNB64955.1"
                     /translation="MVPHLTTALTGPLLELERHFLDNACAIERWMRTQWQEHLPPFYG
                     STDLRNSGFKLAPVDLNLFPGGFNNLNDAFMPLCVQAAQAAIERICVGARAILLIPEN
                     HTRNQFYLQNVA"
     gene            complement(470..1696)
                     /locus_tag="PLH85_10085"
     CDS             complement(470..1696)
                     /locus_tag="PLH85_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB64956.1"
                     /translation="MSAPAPLDVAVLGQVFTPEIVVRAMLRLRHNTGRVLEPACGDGA
                     FLRHLKQVTGIEVDAAHCPPGARRMDFFAFPENEKFDTVIGNPPYVRYQDIPASTRSL
                     LRHDGFDGRSNLYLFFIAKCVRHLAPGGELIFITPRDFLKSTSARQLNRWLYRQGTIT
                     HAIELGDSKVFADAVPNCLIWRFELGNLSHHTAWMAVRQGEALATALESPHWHYRHFS
                     EFAGQLHFSRGEYALSLADVASVRVGAVSGLDELYASDTHGNRDFVSSATARTGLTRR
                     MIWCEPGDPPPAALLPYRDRLLARRIRRFDASNWWQWGRSYPQTDAPRVYVNGKTRAE
                     QPFFLHPCPHFDGAVMGVFPHRPDVDLGAFRDALNRVNWDDLGFVCDGRHLFTQRSLE
                     NAPLPAAFRAFLPDTP"
     gene            2099..2812
                     /locus_tag="PLH85_10090"
     CDS             2099..2812
                     /locus_tag="PLH85_10090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB64957.1"
                     /translation="MPDRGTRTLTLEGDRLQYVVAREHWHYLAMAGSFEQYLADRFSE
                     KTRASFERDEGRFVETFGRLDLREYRSPEELQRFALIAAQVVDGRDRMPALGAMQPGD
                     DAFALFADGAPVAFLHLRSAGDTVQYHSAGESEAFRQWSAGSVLHVAVLRRLFADGRF
                     RYLDFMPGDGEIKRQFANGALRCSDILYLRPTLTNHLLLRLHAYLGRCTAWLDGQAGR
                     AGVVGLLHRLFSREARQVG"
     gene            complement(2831..4045)
                     /gene="hemW"
                     /locus_tag="PLH85_10095"
     CDS             complement(2831..4045)
                     /gene="hemW"
                     /locus_tag="PLH85_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNB64958.1"
                     /translation="MTERRIIPIATQAAPPAGPWVPQLTASPPLALYIHFPWCVRKCP
                     YCDFNSHAVRDEGIPEATYLEALIADLESALPGIWGRRVHSVFIGGGTPSLLSPDGLD
                     ALLTAVRMRVLLDPMAEITLEANPGTVEAGRFRAFREAGVNRLSLGIQSFDDRHLQAL
                     GRIHGSAEARAAAEIALAHFDTVNLDLMYALPGQTLADAEADLATALALGPAHLSCYH
                     LTLEPNTAFAAHPPELPDGDLAADMQDHVESRLSAAGYVHYETSAFARPQRECRHNLN
                     YWTFGDYLGIGAGAHGKLSSHAGILREMRHKHPAAYLAAQQSGDFVQERRTVSGAELP
                     FEFMMNALRLNGGFPLRLFAERTGLPSEAAETGLIEARRRDLVTIEDDWLRPTDQGRR
                     FLNDLLTLFLAD"
     gene            complement(4080..4673)
                     /gene="rdgB"
                     /locus_tag="PLH85_10100"
     CDS             complement(4080..4673)
                     /gene="rdgB"
                     /locus_tag="PLH85_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNB64959.1"
                     /translation="MKQLVLASGNAGKLKELNALLAPLGIEVLPQSAFHVSEAEEPHP
                     TFVENALAKARHASRATGLPALADDSGLCVSALGGAPGVMSARFAGEPKSDARNNSLL
                     LERLDGASDRRARFVCTLALVHHADDPLPLIATGEWHGEILAEARGQSGFGYDPLFYV
                     PDLDQTAAELAPELKNILSHRGAAFRQLLEKLRAELA"
     gene            complement(4695..5432)
                     /gene="rph"
                     /locus_tag="PLH85_10105"
     CDS             complement(4695..5432)
                     /gene="rph"
                     /locus_tag="PLH85_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNB64960.1"
                     /translation="MTDTSFARPSGRAPGELRSVRLTRHFTRHAEGSVLVEFGDTRVL
                     CTASVEESVPPFLRGKGQGWLTAEYGMLPRSTHTRSPREAAKGKQSGRTQEIQRLIGR
                     SLRAVVDLQALGERQITLDCDVLQADGGTRTASITGACVAAWDALAGLVAAGKLTAHP
                     MRDFVAAISVGIYRGVPVLDLDYPEDSDCETDMNVVMTGSGGFVEVQGTAEGAPFSRP
                     ELDALLELAGGGVAQLVREQKKALGLA"
     gene            complement(5429..6337)
                     /locus_tag="PLH85_10110"
     CDS             complement(5429..6337)
                     /locus_tag="PLH85_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HNB64961.1"
                     /translation="MRFTIYQESRVGKRRTNQDRVAYSYTRDALLLVLADGMGGHLYG
                     EVAAQISVQFIAQSFQQEARPKVSDPILFLSRVLSNAHHAILDYAFDKQLSDVPRTTI
                     VACLIQDGVAHWAHAGDSRLYLIRRNKVFAQTRDHSRVQLMLDQGLLDAEAAASHPGR
                     NRIFSCLGGNHPPQIEFSRAVTLANGDVIAMCSDGVWGPIDTDSLVRTLSNGRVMEGV
                     PALMDLSEAAGGAQCDNMSLIAVAWHDELQEDSPETVSTRGMPADAYTTQMEGFGRRR
                     PDQEDLSEDEIEKAIREINAAIHKFK"
     gene            complement(6334..7284)
                     /locus_tag="PLH85_10115"
     CDS             complement(6334..7284)
                     /locus_tag="PLH85_10115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB64962.1"
                     /translation="MATQQNCPLPPGFQLDHYRVEKQLSLGGFSIVYLAYDQDGTPVA
                     IKEYLPNSLALRAQGEITPHVTEAHQSAFRYGMKCFFEEGRALAKLVHPNVVRVLNFF
                     RANGTVYMVMQFERGRTLHDYVQKHKGNIRERFIRGVFTRMLNGLREVHSHKLLHLDI
                     KPSNIYLRADGNPVLLDFGAARQTLASDTPILKPMYTPGFASPEQFTNREALGPWSDI
                     YSVGASMYACLAGTSPQRSDERLNNDTVVPASKLWVGQYSPHFLSTIDWCLQLDPLKR
                     PQSCFALQKVLAQRHREAPPEPKTWLTGLGDRIKTIMGRT"
     gene            7371..8237
                     /locus_tag="PLH85_10120"
     CDS             7371..8237
                     /locus_tag="PLH85_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNB64963.1"
                     /translation="MIHSMTGYAVQTRELGRVALHLELRSVNSRFLDLAFRIADELRQ
                     SEPQLREALTGRLSRGKVECRFHLQGKESATAELVLNGGLLGQLKAVEAAVRAELPAA
                     APLSVGEILRWPGMLGDDGVGFEQIQPQIAPLLAAALEDLVASRRREGEKLADMIRGR
                     IAHMRDLVARVQPRVPALVAEYQEKLAQRLRDASATLDDDRIRLEVGLFAQRVDVAEE
                     LSRLSTHLDEVERILKSGGTAGKRLDFLMQELNREANTLGSKAMAADMTAIAVELKLA
                     IEQMREQVQNIE"
     gene            8243..8857
                     /gene="gmk"
                     /locus_tag="PLH85_10125"
     CDS             8243..8857
                     /gene="gmk"
                     /locus_tag="PLH85_10125"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNB64964.1"
                     /translation="MPGTLFIVTAPSGAGKTTLVSGLLKRDAQVSLSISYTTRTPRAG
                     ETHGVHYHFVDVPTFRALRDKGEFLEWAEVHNNYYGTSRRWLEEQMASGRDILLEIDW
                     QGAQQVRKAFPKAVGVFILPPSVDELERRLRGRGTDSEEVIARRVLGARGEMRHVGEF
                     DYVIINQDLSAALEDLVAVVRAARLRYANQAARQPEFFHYLEQD"
     gene            8861..9076
                     /gene="rpoZ"
                     /locus_tag="PLH85_10130"
     CDS             8861..9076
                     /gene="rpoZ"
                     /locus_tag="PLH85_10130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNB64965.1"
                     /translation="MARITVDDCLKRISNRFQLTLAATYRARQLTAGATPQVELDRHD
                     KDKPTVIALREIAAGKVGTEVLNRGQA"
     gene            9099..11318
                     /locus_tag="PLH85_10135"
     CDS             9099..11318
                     /locus_tag="PLH85_10135"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNB64966.1"
                     /translation="MESAASAPLGRSGVFQPPASSVLPLDFGRLRAQVATYLRPEDVG
                     RIEAAYHFAADAHSGQFRISGDPYISHPVAVAEILAGWHLDAQALCAALLHDVMEDTH
                     ITKSEIAERFGRTTAELVDGVSKLDKIEFQSQEEAQAENFRKMLLAMASDLRVILIKL
                     ADRLHNMRTLDCVRPDKRRRIARETQEIHAPIANRLGLNALYRELQELAFKHVHPMRF
                     RVLSKAILAARGNRREVVGKILAAIEERLPQWNIQAEVRGREKHLYSIYRKMNEKRLS
                     FSQVLDIYGFRVIVPDVQTCYRALGALHGLYKPVPGKFKDYIAIPKANGYQSLHTTLI
                     GPFGTPLEVQIRTAQMNHVADSGVASHWLYKEDEKTLTDLQAKTHTWLQSLLELQSTS
                     GDSSEFLEHVKVDLFPGEVYAFTPKGKIFALPRGATVVDFAYAVHTDVGNRCVAGRVN
                     GELLPLRTELRNGDQVEIITAAHASPNPAWLSYVRTGKARAQIRHFLKNAHQEEAFFT
                     LGERLLNQALRPHGLTLGSVSAIAWERYLREFTLANRKELMTDIGLGRRLSAIVARRL
                     AEIQDIESGRADVVKPKPAGAILIRGTEGVAVQLARCCRPIPGDPIVGIIRKGQGLEV
                     HMHDCPQARKLRGERERWIDVEWEPGVDRLFDVTLRLLTHNVRGVLAKVANAIAEQDC
                     NIQNVSTDGEQGAFSTIYLTVQVTHRLHLAKVIRGVRNIPEVVRIGRLKGDPKLPVA"
     gene            11370..11558
                     /locus_tag="PLH85_10140"
     CDS             11370..11558
                     /locus_tag="PLH85_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HNB64967.1"
                     /translation="MAIYESEHTQFMREWLKNHPEEVSVQQAGRALWWDRPQNPELNE
                     GFAVGKIEQKPYPYSTEG"
     gene            11599..13002
                     /locus_tag="PLH85_10145"
     CDS             11599..13002
                     /locus_tag="PLH85_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB64968.1"
                     /translation="MLAPRLAFIDVETTGANPVVDRVTEIAILRVEDGRLVERWSSLV
                     NPGIPIPPTIQRFTGITDAMVAQAPGFDALKDGVRALFADCVFVAHNARFDYGFIKNE
                     FRRIGEAFDAPVLCTVKLSRALFPQYHRHGLDALIERHGLECTARHRAMGDTDALWQF
                     AQLVCGQFEAPVLETAVLKAMKAPSRPAGLPEGALEGLPEGPGVYLFYGENDLPLYIG
                     KSVSLRSRVMSHFSADHRNAKEARIAQQVRRVDWQTTAGELGALLLESRLVKARKPTE
                     NRQLRETEAVFGLRYDGARKRGPVLLREALNGSDPAAWEAVYGAFRSKREADNALRDL
                     AALYKLCPRRIGTEPWGEGACMAHQMKRCAGVCAGRESPDEHDARLLKVLESLRLRRW
                     PWDGPVAIREHHGETGRSAVHVVDRWCLLGSVEDEGALGELLAAPSPRSFDLDVYRVL
                     SRWLDSPAARGAVTPLG"
     gene            complement(13080..14342)
                     /gene="lysA"
                     /locus_tag="PLH85_10150"
     CDS             complement(13080..14342)
                     /gene="lysA"
                     /locus_tag="PLH85_10150"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNB64969.1"
                     /translation="MTTALPMPTLSAGTAGLHLEGVALASIAERFGTPTYVYSRAALT
                     SAYAAYQQALGERKALVCYAVKANSSLGVLSVFARLGAGFDIVSGGELARVLAAGGDA
                     GKVIFSGVGKTAAEMRFALDAGIGCFNVESAAELDRLNDVAAAAGKRAPIAFRVNPDV
                     NPNTHPYISTGLRSNKFGVAFDEALDLYRKAARLPHIEISGIDCHIGSQLLDPAPMTE
                     AAEKILGLVDQLGREGIKLDHIDLGGGLGIRYRDEEPPTPSIYLKPLLDCFAGRDEAL
                     CFEPGRSLVGNAGLLLTRIEYLKPGVEKNFAIIDAAMNDLARPALYDAYHEIVAVAPR
                     AVPASRYEVVGPICESGDFLGHDRDLSVAAGDLLAILSAGAYGMTMSSNYNTRPRAAE
                     VLVDGDAVHLIRERETLSHLLKGETPLA"
     gene            complement(14339..14503)
                     /locus_tag="PLH85_10155"
     CDS             complement(14339..14503)
                     /locus_tag="PLH85_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNB64970.1"
                     /translation="MRTLSIAALVCLLLSACGIKGPLYLPQVPPPKSKPATQAPAADH
                     NKPAPQDSAP"
     gene            14559..14879
                     /gene="cyaY"
                     /locus_tag="PLH85_10160"
     CDS             14559..14879
                     /gene="cyaY"
                     /locus_tag="PLH85_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HNB64971.1"
                     /translation="MDEARFNALADAELARLEALLDACDVEFDYEVKPGGVLELEFDN
                     GTKIIVNRHTAAREIWLAAKSGGFHFRPQEGRWVGTRDGEDFYAVLSRCMGEQAGVQV
                     RIAG"
     gene            complement(14900..17185)
                     /locus_tag="PLH85_10165"
     CDS             complement(14900..17185)
                     /locus_tag="PLH85_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNB64972.1"
                     /translation="MRWVLYPLTFLLGLVVLGIAVLALAVAFAWPTLPSLEALTDYHP
                     KIPLRVYTADGALIGEYGDERRAFIHIQDVPPVLKNAILAAEDDRFYQHSGVDLLGIL
                     RAAGTNIVTGSKRQGSSTITMQVARNFFLTREKTYSRKLYEILLAFKIEQNLTKDQIL
                     EVYINQIFLGRRAYGFAVAAHTYFGKSIRDLNIPEAAMLAGLPKAPSAYNPIVNPKRA
                     ALRQQYVLRRMTELGFISDAEQQQALATPLKYASSGAEFGGSADYVAEMARQIAVEQF
                     KDEAYTRGIKVITTIDRGEQEAAYRALRRGVMDYDRRHGYRGAEAYVDPTRLGGEQQG
                     ENLDEVLADTPDYDDLKAAVVTQAAPGAVTVYRYGETIRIAGEGLRFAAAMLGDKAPP
                     NKRIRPGAIVRIAQTDNKAWEITQLPEVEGALTAADPRTGAIRALVGGFDFNRNKFNH
                     VTQAWRQPGSSFKPFIYSASFEKGYGPNSIVDDSPLSFPAGETGSQAWEPKNYDGKYD
                     GPISLRTALARSKNMVSIRLLKSIGPRYAQDYVTKFGFDAERHPPYLTMALGAGSVTP
                     WQMLRAYAVFANGGYRVEPYLVKEIRDESGAILARVDPPVAGETAERAIDARNAYLMD
                     SMMKDVVRRGTATRALTLKRGDLAGKTGTTNDYIDAWFCGYQPTVVAVSWIGFDQPRK
                     LGSGETGGAAALPMWIGYMRRALEGVPESYPPAPDGLIRVQPGGGGTEELIYKENLPA
                     VPTEAPVEAPHEGPADEAPPR"
     gene            17359..18435
                     /locus_tag="PLH85_10170"
     CDS             17359..18435
                     /locus_tag="PLH85_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNB64973.1"
                     /translation="MIDLSFLNPKARPLIGVDISSSSVKMVELSGVAGGGYRVERYAI
                     ESLPRDAVVDGNIANLEAVSEAIRRGWRRLGTATKFVAMALPASAVITKKIILPAGLR
                     DQELEVQVESEANQYIPFALDEVNLDFQVLGPAGSGADEDEVLIAASRKEKVEDRVAA
                     AEAAGLKALVMDVESYAALAAFELVQVQLPDEGEDKIIAVVDAGANVMNVSVLRNGNQ
                     VYAREQAFGGYLLTQDIMRHFGMSMEEAENAKRTGALPENYESDLLRPFMDSLALEVT
                     RALQFFFTSTQFNQVDHIVLAGGCAVIPGLAETVAGRSQVDTIVANPFANMTLSSKVR
                     PKNLAADAPALLVACGLALRRFDS"
     gene            18432..19034
                     /locus_tag="PLH85_10175"
     CDS             18432..19034
                     /locus_tag="PLH85_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNB64974.1"
                     /translation="MIRINLLPHREEKRKARRQQFFLLSALMVVAGLFVWFVGHTILG
                     GYLSGQDAKNAFLKEEIAALDKDIVEIKRLREQSEALLSRKQVIESLQTHRTETVAVF
                     NELARLMPEGVYLKALKETGATINLQGFAQSNARVSMLMRNLDGSQYLERPELVEVKA
                     AMINNRRVSEFNMNVSVKRNKADGPGAKGKSDVPQAGGKS"
     gene            19034..19708
                     /locus_tag="PLH85_10180"
     CDS             19034..19708
                     /locus_tag="PLH85_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNB64975.1"
                     /translation="MALKDRIERLRAIDFQALVEDFKGLDPNNPGQWPLAPRGAALVA
                     VFAGIVALGWWFDWSEQMAELDQKTQEESRLKDEWLGKKRQAVNLAEYKRQLGEIDRQ
                     FGALLKQLPNRSEIESLLSDINQAGLGRGLQFELFKPGAEAAKDFYAELPIAIRVTGS
                     YHDLGDFVSDVAQMSRIVTLNDMAIEAGKDGALKFDATAVTYRYLDEEEVAKARKDKS
                     KDKAGK"
     gene            19705..20238
                     /locus_tag="PLH85_10185"
     CDS             19705..20238
                     /locus_tag="PLH85_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNB64976.1"
                     /translation="MRYERLTLLPLLAAALILAGCGDHEDDLRSWMADVSKDLKPNLK
                     PLPPIQQAETVTYQGAGQVDPFRPTKIEPDKKSGMFVPDANRRREPLEAYPLETLKMV
                     GVLIKGGQSHAIIAADKTLHQVHVGNYMGQNYGRVTAISESEVTLKELVEDANGEWVE
                     RVSTLQLQEQAPQEAKK"
     gene            20235..22424
                     /gene="pilQ"
                     /locus_tag="PLH85_10190"
     CDS             20235..22424
                     /gene="pilQ"
                     /locus_tag="PLH85_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNB64977.1"
                     /translation="MRTGNVRVGLGVMFASFWLGCSVPVGLAFAQEPAASTAEASNRI
                     ERILATEQGGNIIVKLSMAQPLSRVPASFSVASPARIAFDFPDTANGLGRNSQTFNTG
                     DLRSANIVQVGDRTRLVLNLARVSPYDARVEGNDVLITLAAAQLKEAAQALPATQFAP
                     VPLNSGDRHAIREINFRRGKDGEGRLVVDLSDPNTGIDIRQQGNALIVDFLKTDVAEQ
                     LRRRLDVVDFGTPVNSVVTQAQGGNVRMTVTPKGLWEHNAYQSDSQFVLEVKPVREDP
                     NKLVKGAGKGQFGGEKLSLNFQNIDVRSVLQVIADFTNFNIITSDSVQGNVTLRLKDV
                     PWDQALDIILQARGLDMRKNGNVIWIAPRDELAAREKMDLESKAQIGDLEPLRTESFQ
                     INYHKAKAVSEFLKSKDQTVLSKRGSVIVDERSNKLFVNDVASRLEDLRRLIAEIDVQ
                     VRQVMIEAQIVEATDNFARNLGMRLGIGGKTGAYLGQTQSSSNSTNSVSVYRNTIGGG
                     LGSTGYQAGQIETKPDFFKDALNLNLPASSINGRQAGAISFALWNSSATRYLDLEVSA
                     LEADGRGKVVSRPRVMTADQAEAVIEQGVEIPYQSATSSGATSVTFRKANLALKVKPQ
                     VTPDGKIMMSLDINKDSPRFDVSLTSGVPIDTKHVKTEVIVENGGTIVIGGIYQQDVS
                     NTTTKVPFLGDLPGLGYFFRNNERKDNKSELLVFITPRILNEQLGLR"
BASE COUNT         3888 a         6772 c         7876 g         3983 t
ORIGIN      
        1 ttggcgacgt tctgcaggta gaactggttg cgggtgtggt tctcggggat cagcaggatg
       61 gcgcgggcgc cgacgcagat gcgctcgatg gcggcctggg cagcctgcac gcagaggggc
      121 atgaaggcgt cgttgaggtt gttgaagccg ccggggaaga ggttgaggtc caccggggcg
      181 agcttgaagc ccgagttgcg caggtcggtg gagccgtaga agggcggcag gtgctcctgc
      241 cattgggtac gcatccagcg ttcgatggcg caggcgttgt cgaggaagtg ccgctccagc
      301 tccagcaggg ggccggtgag cgcggtagtg agatggggaa ccatggaacc tcgcgggctg
      361 ttctcgggtg ggcggcgcgt ttcggtgcgt cgacagggat gtccggcgtg gaccggcgtc
      421 gcccggaatt gttcggcgga tgttaccacc tccgccggaa tgcctttgtt tacggggtgt
      481 ccggcaggaa ggcgcggaag gcggcgggga gcggggcgtt ctcgagactg cgctgggtga
      541 acaggtgacg tccgtcgcag acgaagccca ggtcgtccca gttcacccgg ttgagggcgt
      601 cccggaaggc gcccagatcc acgtccggac ggtgcggaaa cacgcccatc acggcaccat
      661 cgaagtgggg gcagggatgc aggaaaaagg gctgctccgc gcgggtcttg ccattgacgt
      721 agactcgcgg agcgtcggtc tggggatagc tgcgccccca ctgccaccag ttggacgcat
      781 cgaatcggcg gatgcgccgg gcaaggaggc ggtcccggta gggcaacagt gcggcaggcg
      841 gcgggtcgcc cggctcgcac cagatcatgc gccgggtcag gcccgtgcgg gcggtggcag
      901 atgagacgaa gtcccggttg ccgtgggtgt cactggcgta gagctcatcc aggcccgata
      961 ccgccccaac ccgcacgctg gccacgtccg ccagactgag cgcgtactcc ccccgggaaa
     1021 aatgcagctg gccggcaaat tcggagaagt gacggtagtg ccagtggggc gattcgaggg
     1081 ctgtggccag ggcctccccc tgccgcaccg ccatccaggc cgtgtggtgg gacaggttgc
     1141 ccaattcgaa gcgccagatc aggcaattgg ggaccgcgtc ggcgaacacc ttgctgtcgc
     1201 ccagttcaat ggcgtgcgtg atggtgccct ggcggtacag ccagcgattg agctggcggg
     1261 cactggtgga tttgaggaaa tcccgcggcg tgatgaagat cagctcgcct ccgggcgcca
     1321 ggtggcgcac gcacttggcg atgaagaaga gatacaggtt ggaacgcccg tcgaagccgt
     1381 catggcgcag caggctgcgg gtggacgcgg ggatgtcctg gtaccgcacg tagggcgggt
     1441 tgccgatcac ggtgtcgaat ttctcgtttt ccggaaaggc gaagaaatcc atgcggcgtg
     1501 cgcccggcgg acagtgggca gcatccacct cgatgcccgt gacctgtttg agatggcgca
     1561 ggaaggcgcc gtcgccacag gcgggttcga gcacccgtcc ggtgttgtga cgcaggcgaa
     1621 gcatggcgcg caccacgatt tccggcgtaa acacctggcc gagcacggcg acatcaaggg
     1681 gggcaggggc ggacatgggc gggtgagtcg gaataatcct ccattttgcc ccaatgatcg
     1741 gcccgctgca ggggggctgc caggccgccc tgcgggcggg cgggcccgaa ttgtgccaat
     1801 gcgtggcaac ggtacgacga aagtatgatt tgtaatgata tcgtgctatc tttcaaattg
     1861 ttaaagatca tgactggctg cctctctcgc ggccttttgc gggtgcatca aaatgactga
     1921 gcagggtgaa acagaacatc gcctgacatg cggactgggt cggcgggtct tgttctcggt
     1981 ggcaaggcgc gtggccgtgc gggagctttc gctggcggac gtgctggcag gccggagtcc
     2041 ggaccaattg tctgctgccg agctcgcggc gggtcgcgac ggagtcctca ttcgctgcat
     2101 gcccgaccgg gggacgcgga cgctcactct ggaaggcgac cggctgcaat acgtggtggc
     2161 gcgcgagcac tggcactacc tggcgatggc cggcagcttc gagcagtacc ttgcggatcg
     2221 cttctccgaa aagacccggg cgtccttcga gcgggacgag gggcgctttg tcgagacctt
     2281 tggtcgcctc gatttgcggg aataccgcag tccggaagag cttcaacggt ttgcgctgat
     2341 cgcggcccag gttgtggatg gccgcgaccg catgccggcc ctgggggcca tgcagccggg
     2401 ggacgatgct ttcgccctgt ttgccgatgg ggcgccggtg gcgttcctgc acctgcgttc
     2461 ggctggcgac acggttcagt atcacagcgc gggtgaaagc gaggccttcc gccaatggtc
     2521 cgccggctcg gtgctccacg tggcagtcct gcgccgcctg ttcgccgacg gaagattccg
     2581 ctatctggac ttcatgccgg gcgacgggga gatcaaacgt cagttcgcca atggcgccct
     2641 gcgctgttcc gacatcctct acctgcgccc caccctcaca aaccacctgc tcctgcgcct
     2701 gcatgcctat ctgggacggt gcaccgcgtg gctggatggg caggccggac gggcgggggt
     2761 cgtcgggctc ctgcatcgcc tgttcagccg cgaagcccgc caggtcggct gaggcgactt
     2821 ctgcggccgg tcagtccgcc aggaacaggg tcagcaggtc attgaggaat cgccgcccct
     2881 gatcggtggg acgtagccag tcgtcctcga tggtcaccag gtcgcggcgc cgggcctcga
     2941 tcaggccggt ctcggcggcc tcggagggca gtccggtgcg ctctgcgaac agccgcagcg
     3001 gaaagccccc attcaggcgc agggcgttca tcatgaattc gaagggcagt tcagcgccgc
     3061 tcacggtgcg ccgttcctgt acgaaatcgc ctgattgttg cgcggctagg taggcggcgg
     3121 ggtgcttgtg acgcatctcg cgcaggatgc ctgcgtggga cgagagcttg ccgtgggcac
     3181 cggcgccaat ccccagataa tccccaaatg tccagtaatt gaggttgtgg cggcattccc
     3241 gttgagggcg ggcaaaggcg gacgtttcgt agtggacgta gcccgcagcg ctcagacgcg
     3301 attccacgtg gtcctgcatg tcggccgcaa ggtctccgtc gggtagttcc ggcgggtggg
     3361 cggcgaacgc cgtgttgggt tccagcgtga ggtgatagca cgacaggtgc gcagggccga
     3421 gggcgagggc ggtggccagg tcggcctcgg cgtcggccag ggtctggccg ggcagcgcgt
     3481 acatgaggtc gaggttgacc gtgtcgaaat gggcgagggc gatctccgct gcggccctcg
     3541 cctcggcgct cccgtggatg cgtcccaggg cctgcagatg gcggtcgtcg aagctctgga
     3601 tgcccaggga gaggcgattg acgccggctt cgcgaaacgc gcggaagcgt ccggcctcga
     3661 cggtgcccgg attggcctcc agcgtgatct cggccatggg atcaaggagc acccgcatgc
     3721 ggaccgcggt gagcagggcg tcgagcccgt ccgggctgag caggctgggg gtgccgcccc
     3781 cgatgaacac gctgtgcacc cggcgccccc agatgcccgg cagcgcactt tccagatcag
     3841 cgatcagggc ctcgagatag gtcgcttccg ggatgccctc gtcccgcacg gcgtgggaat
     3901 tgaagtcgca gtacggacac ttgcgcacgc accacgggaa gtggatgtac agcgccagag
     3961 gcggcgaggc ggtgagttgc ggcacccacg gcccggcagg cggggcggct tgggtggcaa
     4021 tcgggatgat gcggcgttcg gtcatggcgc gggcggcgcc ggaggagcgc cgcgagggtt
     4081 caggcgagtt cagcgcgcag cttttcgagg agctgacgga aggcggcgcc tcggtgggag
     4141 aggatgttct tcagctcggg cgccagttcg gcggcggtct ggtccagatc gggcacgtag
     4201 aacagagggt cgtagccgaa gccggactgg ccgcgggctt cggcgaggat ctcgccgtgc
     4261 cactcgccgg tggcgatgag gggcaagggg tcatccgcat gatgtacaag ggcgagggtg
     4321 catacgaagc gggcacggcg gtcgctggcg ccgtcaaggc gctccagcag caggctattg
     4381 ttgcgggcgt cggatttggg ttcgccggcg aagcgcgcgg acatcacgcc tggcgcgcca
     4441 ccgagggcgc tcacgcagag gccggagtcg tcggcgaggg ccggcaggcc cgtggcgcgg
     4501 gatgcgtgac gggccttggc aagcgcattt tcgacgaacg tggggtgagg ctcttccgct
     4561 tcgctcacat ggaaggcgga ctggggcagc acctcgatgc ccagcggggc gagcagagca
     4621 ttgagttcct tgagtttgcc ggcgttgccg ctggcaagga cgagttgctt catggtcggc
     4681 ctgctgcgcg ccgctcaggc gagcccgagc gcctttttct gttcacggac cagttgggcc
     4741 acaccgccgc cggcaagttc cagcagggca tccagttcgg gccgggagaa gggtgcccct
     4801 tcagcggtcc cctggacctc gacgaagccc ccgctgccgg tcatgaccac gttcatgtcg
     4861 gtctcgcagt cggagtcttc cgggtagtcc aggtcgagga caggcacccc gcggtagatg
     4921 ccgaccgaga tggcggcgac gaagtcgcgc atggggtggg cggtcaactt gccggcggcg
     4981 acgagaccgg cgagggcgtc ccaggcggcc acgcaggcgc ccgtgatgga ggcggtgcgg
     5041 gtgccgccat ccgcctgcag gacgtcacag tcaagggtga tctggcgttc gccgagggcc
     5101 tgcagatcca ccaccgcacg caggctgcgg ccgatcaggc gctggatctc ctgggtgcgc
     5161 ccgctctgct tgcccttggc ggcttcccgc gggctgcggg tatgggtgga gcggggcagc
     5221 atgccgtatt cggcggtgag ccagccctgg cccttgccgc gcaggaaggg ggggacggac
     5281 tcttcgacac tggcggtgca cagcacgcgg gtgtcaccga attcgaccag caccgaacct
     5341 tccgcgtggc gggtgaaatg gcgggtgagg cggacggagc gcaactcgcc cggggcgcgc
     5401 ccgctcggac gggcaaagga agtgtcagtc atttgaactt gtggatggca gcgttgatct
     5461 cgcggatggc cttttcgatc tcatcctcgc tgagatcctc ctggtccgga cgccgacggc
     5521 cgaagccttc catctgggtg gtgtaggcgt cggccggcat gccgcgggtg gagacggtct
     5581 ccgggctgtc ttcctgcagt tcgtcgtgcc aggcgacggc gatcagggac atgttgtcgc
     5641 actgggcgcc gccggcagct tcggagaggt ccatcagggc cggcacgcct tccatgacgc
     5701 gcccgttgga cagcgtgcgc accagggagt cggtgtcgat ggggccccac acgccgtcgg
     5761 aacacatggc gatgacgtcg ccgttggcca gcgtgacggc ccgtgagaat tcgatctggg
     5821 gtgggtgatt gccgcccagg cagctgaaga tgcggttgcg gccggggtgc gaggcggccg
     5881 cttcagcgtc gagcaggccc tggtcgagca tgagctggac gcgggagtgg tcgcgggtct
     5941 gggcgaacac cttgttgcgg cggatgaggt agagccgcga gtcgcccgcg tgggcccagt
     6001 gggcgacacc gtcctggatc aggcaggcga cgatggtggt gcggggaacg tccgacagct
     6061 gcttgtcgaa ggcgtagtcg aggatcgcgt ggtgggcgtt gctgagcacc cgcgacagga
     6121 acaggatggg gtcgctgacc ttggggcggg cttcctgctg gaaggactgg gcgatgaact
     6181 ggacggagat ctgcgcggcg acctcgccgt acaggtggcc gcccatgccg tcggcgagga
     6241 cgagcaggag cgcgtcgcgg gtataggaat aggcgacccg gtcctggttg gtacggcgtt
     6301 tgccgacccg gctttcctgg tagatcgtga atctcatgta cggcccatga tcgtttttat
     6361 acggtcaccg agtccggtca gccaggtctt gggctccggt ggagcttccc ggtgacgttg
     6421 ggcgaggacc ttttgcaggg cgaagcagct ctgggggcgc ttcaggggat caagctgcag
     6481 gcaccagtcg atggtggaca ggaagtgcgg cgagtactgt ccgacccaca gcttggaggc
     6541 cggcacgacg gtgtcgttgt tcaggcgttc gtcggagcgc tggggcgagg tgcccgccag
     6601 acaggcgtac atgctggcgc cgacgctgta gatgtcgctc catggtccga gcgcttcccg
     6661 gttggtgaac tgctcgggcg acgcgaagcc cggcgtgtac atgggcttga ggatgggggt
     6721 gtcggaggcg agggtctgac gggctgcgcc gaaatcaagg agcaccgggt tgccgtccgc
     6781 ccgcagatag atgttggacg gcttgatgtc caggtgcagc agcttgtggg aatgcacctc
     6841 tcgcagtccg ttgagcatgc gggtgaagac cccgcggatg aagcgctcgc ggatgttgcc
     6901 cttgtgcttc tggacgtagt catgcaaggt tcggccgcgt tcgaactgca tcaccatgta
     6961 aaccgtaccg ttggcgcgaa agaagttgag cacccgcacg acattagggt gcaccagctt
     7021 ggccagcgcc cggccttcct cgaagaagca cttcatgccg tagcggaagg cactctggtg
     7081 cgcttcggtg acatgggggg tgatctctcc ctgggcgcgt aacgcgaggg aattgggcag
     7141 atattccttg atggccaccg gcgtgccgtc ctggtcgtag gccaggtaga caatcgaaaa
     7201 tccgcccagg gacaactgct tctcgacacg gtagtggtcg agctggaatc cgggtggcag
     7261 ggggcagttt tgttgagtgg ccatcgggcg gggcgctaca cttcgttcct ttgggcggca
     7321 gggtgcttag tttagcgccg gattgcttcg tttattcggg aaaatacaag atgattcaca
     7381 gcatgacggg ttacgccgtt cagacccgcg aactggggcg cgtggcgctc catctggagc
     7441 ttcgcagcgt caattcccgc tttcttgatc tggctttccg catcgcggat gagctgcgcc
     7501 agtccgagcc tcaactgcgg gaagcgctga ccggtcggct cagccggggc aaggtcgagt
     7561 gccgcttcca cctgcagggc aaggagagcg caacggccga actggtgctc aatggtgggc
     7621 tgctcggtca gttgaaggcg gtcgaggccg cggtgcgggc cgagttgccc gccgccgcgc
     7681 ccctgagcgt tggcgagatc ctgcgctggc ccggcatgct gggggatgac ggcgtcgggt
     7741 tcgaacagat ccagccgcag atcgcgccct tgctcgccgc tgcgctggag gatctcgtcg
     7801 cctcgcgccg tcgcgagggc gagaagctcg ccgacatgat ccgtgggcgc atcgcccaca
     7861 tgcgcgatct ggtggcccgc gtccagcccc gtgtgccggc gctggtcgcc gaataccagg
     7921 aaaagctcgc ccagcgcctg cgcgacgcgt ccgcgaccct cgatgatgac cgtatccggc
     7981 tggaagtcgg gctgttcgcc cagcgcgttg acgtggcgga ggaactgtcg cgcctgagca
     8041 cccaccttga cgaggtggag cgcatcctca agagcggcgg taccgccggc aagcgcctgg
     8101 acttcctaat gcaggagctc aatcgcgagg ccaacacgct cggctccaag gccatggctg
     8161 ccgacatgac tgcgatcgcc gtggaactca agctggccat cgaacagatg cgcgagcagg
     8221 tccagaacat cgagtgacgc ccatgcccgg aaccctgttc atcgtcacgg cgccatctgg
     8281 cgccggcaag accactctgg tgagcggcct gctcaaacgg gatgcccagg tcagtctgtc
     8341 catctcgtat accacccgga cgccgcgagc cggcgagaca catggcgtgc attaccactt
     8401 cgtggacgtg ccgacgttcc gtgccttgcg cgacaaaggg gaatttctcg aatgggcgga
     8461 ggtccacaac aattattacg ggacatcccg gcgctggctc gaggagcaga tggcatcggg
     8521 gcgggacatt ctgctggaga tcgactggca gggtgcccag caggtgcgca aggcgttccc
     8581 caaggccgtc ggggtgttca tcctgccgcc gtccgtggat gaactcgagc gtcgcctgcg
     8641 cggccgcggc accgacagcg aggaggtcat cgcccggcgc gtgctcggtg cccgcggaga
     8701 gatgcggcat gtcggggagt tcgactacgt tataatcaac caagacctgt ccgcggcgct
     8761 cgaagatctg gtggccgttg tgcgggcagc gcgcttgcgc tacgccaatc aggcggcgcg
     8821 ccagcccgag tttttccact atctggagca ggattgatcc atggcccgta tcaccgtcga
     8881 tgattgtctg aagaggattt ccaaccgttt ccagctcacc ctggcggcga cctaccgtgc
     8941 ccgtcagctg acggccggtg ccacgcccca ggtggagctt gaccgtcacg acaaggacaa
     9001 acccaccgtc atcgccctgc gcgaaatcgc cgccggcaag gtggggacgg aagtcctgaa
     9061 ccgcggtcag gcctgatcct caccgagcgg gcaatgtcat ggaatccgcc gcatcggctc
     9121 cgcttggacg cagcggcgtt ttccagcctc cggcctcaag tgtgctcccg ctcgatttcg
     9181 gtcgcctgcg tgcccaggtg gcgacctacc tccgtccgga ggacgtaggc cgcatcgagg
     9241 cggcctatca cttcgccgcg gacgcccatt cggggcagtt ccgcatcagt ggcgatcctt
     9301 acatctccca tcccgtcgcc gtcgccgaga ttctcgccgg ctggcatctg gacgcccagg
     9361 ccttgtgcgc ggcgctcctc cacgacgtga tggaggacac ccacatcacc aagtccgaga
     9421 tcgcagagcg tttcggaagg accaccgccg aactggtgga tggcgtgtcc aagctcgaca
     9481 agatcgaatt ccagtcccag gaagaggcgc aggccgagaa tttccgcaag atgctgctgg
     9541 ccatggccag cgacttacgg gtcatcctga tcaagctcgc cgaccgcctc cacaacatgc
     9601 gtacgctcga ctgcgtgcgt ccggacaagc gccgccgcat cgctcgcgag acccaggaga
     9661 tccacgcccc catcgccaac cggctgggtc tcaacgccct ctaccgggaa ttgcaggagc
     9721 tggccttcaa gcacgtccac ccgatgcgct tccgggtgct ctccaaggcc atcctggcgg
     9781 cgcggggcaa tcgccgcgag gtggtcggca agatcctcgc ggccatcgag gagcggctgc
     9841 cccagtggaa catccaggcc gaggtgcggg gccgcgagaa gcacctgtac agcatctacc
     9901 gcaagatgaa cgagaagcgc ctgtcctttt cgcaggtgct ggacatctac ggcttccgcg
     9961 tcatcgtgcc tgacgtgcag acctgctacc gcgccctcgg tgcgctgcat gggctgtaca
    10021 agccggtgcc gggcaagttc aaggactaca tcgccattcc caaggcgaac ggttatcagt
    10081 ccctgcacac caccctgatc gggcctttcg gtacgccgtt ggaggtgcag atccgtaccg
    10141 cccagatgaa ccacgtggcc gacagcggcg tggcctccca ctggctgtac aaggaagacg
    10201 agaagaccct cactgacttg caggccaaga cccacacctg gctgcagtcc ctgctggaac
    10261 tccaatccac ctccggcgat tcgagcgagt tcctcgaaca cgtgaaggtg gatctctttc
    10321 ccggcgaggt ctacgccttc acgcccaagg gcaagatctt tgccctgccg cggggcgcga
    10381 cggtggtgga cttcgcctac gcggtgcaca ccgacgtggg taaccgctgc gtggccggcc
    10441 gcgtcaatgg cgaactcctg cccctgcgca ccgagctgcg caatggcgac caggtggaga
    10501 tcatcaccgc cgcccacgcc agccccaacc cggcctggct cagttatgtg cgcaccggca
    10561 aggcgcgggc gcagatccgc catttcctca agaacgccca ccaggaagag gccttcttca
    10621 ccctcggcga gcgcctgctc aaccaggccc tgcgtcctca tgggctcacc ctgggttccg
    10681 tcagtgcgat tgcctgggaa cgttacctgc gggaattcac tctggccaac cgcaaggagc
    10741 tgatgaccga catcgggctc ggtcggcgcc tgtccgccat cgtggcgcgg cgtctggcag
    10801 agatccagga catcgaatcg ggccgcgccg acgtggtcaa gcccaaaccg gccggtgcca
    10861 tcctgattcg tggcaccgag ggtgtggcgg tgcaactggc gcgctgctgc aggccgatcc
    10921 ccggcgatcc catcgtcggc atcatccgca agggccaggg gctggaagtg cacatgcacg
    10981 actgtcccca ggcccgcaag ctgcgcggtg agcgggagcg ctggatcgac gtggagtggg
    11041 aaccgggcgt ggaccgcctc ttcgacgtga ccctgcgtct gctcacgcac aatgtgcgcg
    11101 gggtgctggc caaggtggcc aatgccatcg ccgagcagga ctgcaacatc cagaatgtca
    11161 gcaccgatgg ggagcagggt gccttctcca cgatctacct caccgtgcag gtcacccacc
    11221 gtctgcatct ggccaaggtc atccgcggcg tgcgcaacat tccggaagtg gtgcgcatcg
    11281 gccgtctgaa gggtgacccc aagctgccgg tcgcctgaga ccgccactga ctgctgctag
    11341 aatccacgtc atttcaaggg agatttcata tggccatcta cgaatccgaa cacacccaat
    11401 tcatgcgcga atggttgaag aaccacccgg aagaggtctc tgtgcagcag gcgggtcgcg
    11461 ccctgtggtg ggaccgtccg cagaatcccg agctgaacga gggattcgcc gttgggaaga
    11521 ttgaacagaa gccttacccg tactccactg aaggctgagt ccgccgccct gcccggttgt
    11581 ccccctgctc cacacgtcgt gctcgcaccc cggctcgctt tcatcgatgt ggaaaccacc
    11641 ggggccaatc cggtggtgga ccgggtcacc gagatcgcca tcctgcgggt ggaagatggc
    11701 cggctggtgg agcgctggtc cagtctggtc aatccgggca tcccgatccc gcccaccatc
    11761 cagcgtttca ccggcattac cgacgccatg gtggcgcagg cgccaggctt tgacgcgctc
    11821 aaggacggcg tgcgggcact cttcgccgac tgcgtgttcg tcgcccataa cgcacgcttt
    11881 gactacggct tcatcaagaa cgaattccgc cgcatcggcg aggcgttcga cgcgccggtg
    11941 ttgtgcacgg tcaagctgtc ccgcgccctg tttccccagt atcaccgcca cggcctggat
    12001 gcgctgatcg agcgccacgg cctcgaatgc accgcccgtc accgggccat gggcgatacc
    12061 gacgctttgt ggcagttcgc ccaactggtt tgcggccagt tcgaagcacc ggtgctggaa
    12121 acggccgtcc tcaaggccat gaaggcgccg agccggccag ccgggctgcc ggaaggcgcg
    12181 ctggaaggct tgccggaagg gcctggcgtg tatctgttct acggcgagaa cgacctgccc
    12241 ctctacatcg gcaagagtgt cagcctgcgc tcgcgggtga tgagccattt ttctgcagat
    12301 caccgcaacg ccaaggaggc gcgtatcgcc cagcaggtgc ggcgggtgga ctggcagacc
    12361 acggccggcg agctcggtgc gctgctcctg gagtcgcgcc tcgtcaaggc ccgcaaaccc
    12421 accgagaacc gccagctgcg tgaaaccgag gcggtcttcg gtctgcgcta cgacggcgcc
    12481 cgcaagcgtg gcccggtgct cctgcgggag gcgctcaacg gcagcgatcc ggccgcctgg
    12541 gaggccgtgt atggcgcctt ccgcagcaag cgcgaggccg acaacgcatt gcgggatctg
    12601 gcggccctgt acaagctgtg cccgcgccgc atcggcacgg agccgtgggg cgaaggggct
    12661 tgcatggctc accagatgaa gcgctgcgcg ggtgtctgcg cgggtcggga aagccctgat
    12721 gagcacgatg cgcgtctcct gaaggtgctg gagagcctgc gtctgcgccg ctggccatgg
    12781 gacggcccgg tggcgatccg cgaacaccac ggggagaccg gtcgcagcgc ggtgcacgtg
    12841 gtggatcgct ggtgcctgct gggcagcgtg gaggacgagg gcgcgctggg cgagctgctg
    12901 gcggcgccgt cgccacgcag cttcgatctg gacgtctacc gcgtcctgtc gcgctggctc
    12961 gattcgccgg cggctcgtgg ggcggtcacg ccgctggggt gagcgcgtac ggcggacctc
    13021 atgacaaaaa cccggcatgg ccgggttttt ttgtgggtgt tgtggtgccg gccttcgcgt
    13081 taggccagcg gcgtttcgcc cttgagcagg tggctgaggg tttcccgttc gcggatcaga
    13141 tgcaccgcgt cgccgtccac cagcacttcc gccgcccgcg ggcgggtgtt gtagttggag
    13201 ctcatggtca tgccgtaggc cccggcggac aggatggcca gcaggtcgcc cgcggccacc
    13261 gacaggtcgc ggtcgtggcc aaggaagtcg ccgctctcgc agatcgggcc caccacttcg
    13321 tagcgggatg ccggcacggc gcggggtgcc acggcgacga tctcgtgata ggcgtcgtag
    13381 agcgccgggc gggccagatc gttcatggcc gcgtcgatga tggcgaagtt cttctcgacg
    13441 ccgggcttga ggtattcgat gcgggtcagg agcaggcccg cgttgcccac cagggagcga
    13501 ccgggctcga agcacagggc ctcgtcgcgg ccggcgaagc agtccagcag gggcttgagg
    13561 tagatgctcg gggtgggcgg ttcctcgtca cggtagcgga tgccgaggcc gccgcccaga
    13621 tcgatgtggt cgagcttgat gccttcgcgg ccgagctgat ccaccaggcc gaggatcttc
    13681 tcggcggctt cggtcatggg ggccgggtcg aggagctggg agccgatgtg gcagtcgatg
    13741 ccggagatct cgatgtgggg caggcgcgcc gccttgcgat acagatcaag ggcctcgtcg
    13801 aaggccacgc cgaacttgtt gctgcgcagc cccgtggaga tgtaggggtg ggtgttggga
    13861 ttgacgtccg gattgacgcg gaaggcaatc ggcgcgcgct tgcccgccgc ggcggcgacg
    13921 tcattcaggc ggtcgagttc ggcggcggat tccacgttga agcagccgat gccggcgtcc
    13981 agcgcaaagc gcatctcggc agcggtcttg ccgacaccgg agaagatcac cttgccggca
    14041 tcgccacccg cagccagtac gcgggccagt tccccgccgg agacgatgtc gaagccggcg
    14101 cccagcctgg cgaacaccga caagacgccc aggctggagt tggccttgac ggcgtaacac
    14161 accagcgcct tgcgttcgcc gagggcctgc tggtaggccg cgtaggcgga ggtgagggcg
    14221 gcacgggaat agacgtaggt gggcgttccg aaacgttcgg cgatgctggc aagggccacg
    14281 ccttcgaggt gcaggccggc ggtgccggcg ctgagcgtcg gcatgggcaa agcggtggtc
    14341 atggggcgga atcctgggga gcgggtttgt tatgatcggc ggcgggtgcc tgtgtggccg
    14401 gcttcgactt gggcggcggc acctggggca gatagagcgg gcccttgatg ccacaggcgc
    14461 tcaggagcag gcagacgagg gcagcgatgg aaagtgtgcg catgggtcgc atgggtcgcg
    14521 cgcaaaaacg acaatgttaa cagatcggga agatggccat ggatgaagca cgattcaacg
    14581 cactggcaga cgcggaactg gcgcgtctgg aagccttgct cgatgcctgc gatgtggagt
    14641 tcgactatga agtgaagccg ggcggtgtgt tggaactgga gttcgacaat ggcacgaaga
    14701 tcatcgtgaa ccggcatacg gcggcgcggg agatctggct ggcggccaag tcgggtggct
    14761 tccatttccg gccccaggag ggccgctggg tgggcacgcg ggacggcgag gatttctatg
    14821 ccgtgctgtc ccgctgcatg ggcgaacagg ctggcgtgca ggtgcgcatc gcaggctgaa
    14881 gccggtgtgc cgcacccgct cagcggggcg gcgcttcgtc ggccgggccc tcatggggcg
    14941 cctcgacggg ggcttcagtc gggaccgccg gcaggttttc cttgtagatc agttcttcgg
    15001 ttccgccgcc accgggctgt acgcggatca ggccgtcggg tgcgggcggg tagctttcgg
    15061 gcacgccttc gagcgcgcgg cgcatgtagc cgatccacat gggcagcgcg gcggcgccgc
    15121 cggtttcgcc ggagccaagc ttgcgcggct ggtcgaaacc gatccatgac acggccacca
    15181 cggtaggttg atagccgcag aaccaggcgt cgatgtagtc gttggtcgtg ccggtcttgc
    15241 cggccaggtc gccgcgcttg agggtcagcg cgcgggtggc ggtgccgcgc cggaccacgt
    15301 ccttcatcat ggagtccatc agataggcgt tgcgggcgtc gatggcccgc tcggccgttt
    15361 cgcccgccac aggcggatcg acgcgggcga ggatcgcgcc ggattcatcg cggatctcct
    15421 ttaccagata gggttcgacg cgatagccgc cgttggcgaa gacggcatag gcgcgcagca
    15481 tctgccacgg cgtaacggag ccggcgccca gggccatggt gaggtagggc gggtggcgtt
    15541 cggcgtcgaa gccgaacttg gtcacgtagt cctgggcgta gcgcggaccg atggatttga
    15601 gcaggcggat cgacaccatg ttcttggagc gagccagggc cgtacgcagg gaaatggggc
    15661 cgtcgtactt gccgtcgtag ttcttgggtt cccaggcctg gctgccggtc tcgcccgccg
    15721 gaaaggacag gggcgaatca tccacgatgc tgttggggcc gtaacccttt tcgaaggagg
    15781 cggagtagat gaagggcttg aagctggagc cgggctgccg ccaggcctgg gtgacgtggt
    15841 tgaacttatt gcggttgaag tcgaagccgc ccaccagggc gcggatggcg ccagtgcgcg
    15901 ggtcggcggc ggtgagcgcg ccttcgacct cgggcagttg ggtgatctcc caggccttgt
    15961 tgtccgtctg ggcaatgcgc acgatggcgc cggggcggat gcgcttgttg ggcggggcct
    16021 tgtcgccgag catcgccgca gcgaagcgca gaccttcgcc cgcgatgcgg atcgtttcac
    16081 cgtagcggta gaccgtgacg gcgcccggtg cggcctgggt gacgacggcg gccttcaggt
    16141 cgtcgtagtc gggggtgtcg gcgaggactt cgtcgaggtt ttcgccctgc tgctcgccgc
    16201 ccaggcgggt ggggtcgacg taggcctcgg cgccgcggta gccgtggcgg cggtcgtagt
    16261 ccatcacgcc gcggcgcagg gcccgatagg cggcttcctg ctcgccccga tcgatggtgg
    16321 tgatgacctt gatgccgcgg gtgtaggcct cgtccttgaa ctgttccacg gcgatctggc
    16381 gcgccatctc ggccacgtaa tcggcgctgc cgccgaattc ggcgccgctg gaagcatatt
    16441 tgagcggcgt ggcgagggcc tgctgctgct ccgcatcgga gatgaagccg agttcagtca
    16501 tgcggcgcag gacatattgc tggcgcaggg ccgcgcgctt ggggttgacg atggggttgt
    16561 aggcggaagg cgccttgggc aggccggcca gcatggcggc ttcgggaatg ttcagatccc
    16621 ggatggattt gccgaaatag gtgtgggcgg cgacggcaaa gccgtaggcg cggcggccca
    16681 ggaagatctg attgatgtag acctcgagga tctggtcctt ggtaaggttc tgctcgatct
    16741 tgaaggcgag caggatttcg taaagtttcc gggagtaggt tttctcccgg gtgaggaaga
    16801 agttgcgggc cacctgcatg gtgatggtgg aggagccctg gcgtttgctg ccggtgacga
    16861 tgttggtgcc ggcagcccgc aggatgccga gcaggtccac gccggaatgt tgatagaagc
    16921 ggtcgtcctc ggcggcgagg atggcattct tgaggacagg cgggacgtcc tgtatatgga
    16981 tgaaggcgcg acgttcgtcc ccgtattcgc cgatcagcgc gccatcggct gtgtagacgc
    17041 gtagcgggat cttggggtga tagtccgtca gcgcttccag ggacggcagg gtcggccagg
    17101 caaaggcaac cgccagggca aggacagcga tgcccagcac gacgaggccg agcaggaagg
    17161 tcagggggta cagtacccag cgcatggagt gtccggcaga cggaaacggt tcgccattat
    17221 acggggaggc ccgcctcgtc ggccgttgcg tgtggcgtgg ttttacccgg gcttgcttta
    17281 cacccggcat ggttgttgtt aggatttcaa gtagattgtt gttattgcgc ctaacgttac
    17341 gaattttcag gactttttgt gatcgaccta tcctttctca atccgaaggc gcggcctttg
    17401 atcggcgtcg acatttcgtc gtcgtcggtc aagatggtcg aactgtccgg cgtggcgggc
    17461 ggagggtacc gggtcgaacg ctacgcgatc gaatccctgc cgcgtgatgc ggtcgtcgac
    17521 ggcaacatcg ccaacctgga agcggtgtcc gaagccatcc ggcgcggctg gcggcgcctg
    17581 gggacggcca cgaaattcgt cgccatggca ttgccggcgt cggcggtcat caccaagaag
    17641 atcatcctgc cggcagggtt gcgtgaccag gagctcgaag tccaggtcga gtccgaggcc
    17701 aatcagtaca ttccctttgc gctggacgag gtcaatctgg attttcaggt gctcggcccg
    17761 gcgggctccg gtgcggatga ggacgaagtg ctgattgcag cgtcccgcaa ggaaaaggtg
    17821 gaggaccgtg tggcggcggc ggaggcggcg ggtctcaagg cgctggtgat ggatgtggag
    17881 tcctatgcgg cgctggcagc attcgagctg gtccaggtgc agttgcccga cgaaggggag
    17941 gacaagatca tcgccgtcgt ggacgcgggc gccaatgtca tgaacgtctc ggtgctgcgc
    18001 aacggcaatc aggtgtatgc gcgggagcag gccttcggtg gctacctcct cacccaggac
    18061 atcatgcgtc acttcggcat gtccatggaa gaggctgaaa acgccaagcg taccggcgcc
    18121 ctgcccgaga actatgaatc cgatctgctg cgccccttca tggacagcct ggcgctggaa
    18181 gtgacgcgcg ccttgcagtt cttcttcacc tcgacccagt tcaaccaggt cgatcacatc
    18241 gtgctcgccg gagggtgtgc ggtcattccc gggctggcgg agacggtggc cgggcgatcc
    18301 caggtggaca ccatcgttgc caacccgttc gccaacatga ccctttcctc gaaggtccgc
    18361 cccaagaacc tcgcagccga tgcgccggcc ttgctggtgg cgtgcggact ggccctgcgg
    18421 aggtttgatt cgtgatccgc atcaacctgc tcccgcaccg ggaggaaaaa cgcaaggcga
    18481 gacggcagca gttcttcctg ctgtcggccc tgatggtggt ggccgggctg ttcgtgtggt
    18541 tcgtcggaca tacgatcctg ggcggctacc tcagcggaca ggatgccaag aatgccttcc
    18601 tcaaggagga aatcgcggcc ctcgacaagg atatcgtcga gatcaagcga ctgcgggagc
    18661 agtccgaggc gctgctgtcg cgcaagcagg tgatcgaatc cttgcagacg caccgtaccg
    18721 agaccgtcgc ggtctttaac gaattggccc gactaatgcc agagggggtt tatctcaagg
    18781 cactcaagga aaccggcgcg acgatcaatc tgcagggatt tgcgcagtcg aacgcgcggg
    18841 tctcgatgct gatgcgcaat ctggacgggt cgcaatatct cgaacgtccc gaactggttg
    18901 aagtgaaggc ggcgatgatc aataaccgtc gggtcagtga gttcaacatg aatgtttccg
    18961 tcaagcgcaa caaagccgac ggccccggcg ccaagggcaa gtcggacgtt ccgcaggcgg
    19021 gagggaagtc gtgatggcac tcaaggatcg tatcgaacgc ttgcgtgcca tcgatttcca
    19081 ggccctcgtc gaggatttca aggggcttga tccgaacaac cccggacaat ggcccctcgc
    19141 gccgcgcggt gctgccttgg tggcggtctt tgccgggatt gtggcactgg gctggtggtt
    19201 cgactggagc gagcagatgg ccgaactgga tcagaagacc caggaggaaa gccgtctcaa
    19261 ggatgaatgg ctgggcaaga agcggcaggc ggtgaacctg gcagagtaca agcgccagct
    19321 tggcgagatc gaccggcagt tcggtgcgct gctgaagcag ctccccaatc ggtccgagat
    19381 cgaaagcctg ctgtcggaca tcaatcaggc tggactcggg cgtggtctgc agtttgaact
    19441 cttcaaaccc ggggccgaag ccgccaagga tttctacgcc gaactgccga tcgccatccg
    19501 ggtgaccggg tcctatcacg acctgggcga tttcgtcagt gatgtggccc agatgtcgcg
    19561 catcgtcacg ctcaatgata tggccatcga ggccggcaag gatggagcac tcaagttcga
    19621 cgcgacggcc gtgacctacc gctacctgga tgaagaggaa gtggccaagg cgcgcaagga
    19681 caagtccaag gataaggcgg gcaagtgaga tacgagcgac tgacccttct gccgctgctg
    19741 gcggccgcgt tgatcctcgc gggctgcggt gatcatgagg acgacctgag gagctggatg
    19801 gcggatgtct ccaaggacct caagcccaat ctcaagccct tgccacccat ccagcaggct
    19861 gagacggtga cctatcaggg ggcagggcag gtggatccgt tccgcccgac caagattgaa
    19921 cccgacaaga aatccggcat gttcgtgccg gatgcgaacc ggaggcgtga gcctctcgaa
    19981 gcttacccgc tcgaaaccct caagatggtg ggggtcctga tcaagggcgg gcagtctcat
    20041 gcgatcatcg cggccgacaa gacgctgcat caggtgcacg tcggcaatta catgggacag
    20101 aactacggtc gggtgacggc aatcagtgag tccgaagtca ccctcaagga actcgtcgag
    20161 gatgcgaacg gcgaatgggt cgagcgcgtc agtacgctgc agttgcagga gcaggcgccc
    20221 caggaggcga agaaatgaga acaggaaatg tcagggtcgg tctgggcgtg atgtttgcgt
    20281 cgttctggct gggatgcagc gtgccggtcg gactggcgtt tgcccaggag ccggctgcct
    20341 cgactgctga agcgagcaac cgtatcgagc ggatccttgc caccgagcaa gggggcaaca
    20401 tcatcgtcaa gctgtccatg gcgcagccct tgtcacgtgt gccggccagc ttcagtgtgg
    20461 cgtctcctgc acggattgcc ttcgatttcc ccgatacggc caatgggctc ggtcgtaaca
    20521 gtcagacctt caataccggc gacctgcgca gcgccaatat cgtacaggtc ggtgaccgca
    20581 cgcgcctggt cctgaacctc gcgcgggtgt caccctatga tgcgcgggtc gaggggaatg
    20641 atgtgctgat caccctggcg gccgcccagc tcaaggaggc tgcgcaggca ttgccggcga
    20701 cccagttcgc gccggtgcca ctcaattccg gcgatcgcca cgccatccgt gaaatcaatt
    20761 tccggcgtgg caaggacgga gaagggcgtc tggtcgtgga tctttcggat cccaacacgg
    20821 gcatcgatat ccgccagcag ggcaacgccc tcatcgtcga tttccttaag accgatgtgg
    20881 ccgagcaatt gcgccgcagg ctcgatgtgg tggatttcgg gacacccgtc aactcggtgg
    20941 tgacccaggc gcaaggcggc aatgtgcgca tgaccgttac gcccaagggc ctctgggagc
    21001 ataacgccta ccagagtgac agccagttcg tcttggaggt caagcccgtt cgcgaggatc
    21061 ccaacaagct cgtcaagggg gccggcaagg ggcagttcgg tggagaaaag ctctccctca
    21121 acttccagaa tatcgatgtt cgttcggtgc tgcaggtgat cgccgacttc accaacttca
    21181 atatcatcac cagcgattcc gttcagggta acgtcaccct ccgcctcaag gacgtgccct
    21241 gggaccaggc acttgacatc atcctgcagg ctcgcggcct cgacatgcgc aagaatggca
    21301 acgtgatctg gatcgcgccg agggacgaac tggccgcccg ggaaaagatg gatcttgagt
    21361 ccaaggccca gatcggcgat ctggagcccc tgcgcacgga aagcttccag atcaactacc
    21421 acaaggccaa ggcggtttcg gagttcctca agagcaagga ccagacggtg ctgtccaagc
    21481 gtggcagcgt gattgttgac gagcgcagca acaagctctt cgtcaatgac gtggcgtccc
    21541 ggctcgagga tctccgtcgc ctgatcgccg agattgacgt gcaggtgcgc caggtcatga
    21601 tcgaggcgca gatcgtcgaa gcgacggaca acttcgcacg caacctgggc atgcgtctgg
    21661 gcatcggcgg caagaccggt gcctacctcg gtcagaccca gtcctcgagc aactccacca
    21721 attcggtctc cgtctatcgc aacaccatcg gcggtgggct ggggtcgacc ggttaccagg
    21781 ccgggcagat cgagaccaag ccggatttct tcaaggacgc gctcaacctc aaccttccgg
    21841 cttccagcat caacggtcgt caggcgggcg ccatctcctt cgcgttgtgg aacagctcgg
    21901 cgacccgtta cctggacctg gaagtgtcgg ccctcgaagc cgatggtcgc ggcaaggtcg
    21961 tttcccgtcc gcgggtcatg acggccgatc aggctgaggc ggtgatcgag cagggcgtcg
    22021 agatccccta ccagtcggcg accagctcgg gtgccacgtc cgtgaccttc cgcaaggcaa
    22081 acctggccct caaggtcaag ccgcaggtca cccccgacgg caagatcatg atgtccctcg
    22141 acatcaacaa ggattctccc cggtttgacg tctccctcac gtccggggtg ccgatcgaca
    22201 ccaagcacgt gaagaccgag gtcatcgtcg agaacggcgg taccatcgtc attggcggca
    22261 tttatcagca ggacgtctcc aacacgacga cgaaggtccc gttcctcggc gatctgcccg
    22321 gtctcggtta tttcttcaga aacaacgaac gcaaggacaa caagtccgaa cttctggtct
    22381 tcatcacgcc ccgtatcctc aacgagcagc tgggcctgcg ttaatccgga ctctggatgg
    22441 aacacctcat gcatcaatcg attctgcgca cgtttttgtc ctgggccctg ctgggggttc
    22501 tgggggtggt gatgagcct
//
LOCUS       DAOQMM010000055        22245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_786_length_22245_cov_43.803245, whole genome shotgun sequence.
ACCESSION   DAOQMM010000055 DAOQMM010000000
VERSION     DAOQMM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 22245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22245
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_22245_cov_43.803245"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..585)
                     /locus_tag="PLH85_10195"
     CDS             complement(19..585)
                     /locus_tag="PLH85_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNB64978.1"
                     /translation="MRHMFIAALALSAGIAQAADLDALKSETRQKALPVLPKVAGMMK
                     ETVVAEGTVAAIPVCKEKAPQLLRALSAETGWDIRRVSLKTRNPQRGTPDVWEARQLA
                     DFNIRAANGEKVETLETGEIVSEHGKPVYRYIRAIPAGDVCLACHGAADTLDAPLREQ
                     LARHYPDDRATGYTKGQIRGALTVKRPL"
     gene            complement(620..1198)
                     /locus_tag="PLH85_10200"
     CDS             complement(620..1198)
                     /locus_tag="PLH85_10200"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB64979.1"
                     /translation="MSLADWVAHYGYFAVAVGCLLEGETVLLLAGVAVHRGLLDLPAV
                     AAVAFVAGFAGDQAWFFVGRRFGDRLIRRFAAVQRAVPRIQDLILRHQNLLVLGIRFM
                     IGLRIAGPVLMGWSRVQALRFVLLNLLGAALWVAVIGGGGYYFSALMERLLPDLRRAE
                     EGLLAATAAAGLVAHGVRLWRGRAARGRNKVY"
     gene            complement(1195..1752)
                     /locus_tag="PLH85_10205"
     CDS             complement(1195..1752)
                     /locus_tag="PLH85_10205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNB64980.1"
                     /translation="MSSPLKIVTLLGSLRQGSYNAAIARALPTVAGEDVVVEALPSIR
                     DIPVYDADLQAEGFPPAVIALADAIRAADGVVIVTPEYNYSVPGALKNAIDWLSRVPE
                     QPFAGKPVAIQTASMGILGGARCQYHLRQVLVFLDCHVLNKPEVMIGAVQDKVNAAGE
                     LADTGTLKRLDEQLLAFTKFIRRLG"
     gene            1913..2143
                     /locus_tag="PLH85_10210"
     CDS             1913..2143
                     /locus_tag="PLH85_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HNB64981.1"
                     /translation="MQAPTHTLGHLFEQLGLPADTGAIEAFLDSHSLPPDMRLADAPF
                     WTKPQADFLARELADDADWAELIDSLDARLRH"
     gene            complement(2151..3377)
                     /locus_tag="PLH85_10215"
     CDS             complement(2151..3377)
                     /locus_tag="PLH85_10215"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNB64982.1"
                     /translation="MLKKIPVERLRAGMFIHELCGDWMSHPFWRARFRLNGGDDLQRI
                     VESGIRHVYIDTDKGLDDAEAVDAGAVREAVEAQMVAALATPEETPLRVSVAEEMERA
                     RRVHEQAHRVVRGMMQDVRLGRAVSLEDAEPVVEAITGSVLRNSGALIGLIGIKNKDD
                     YTFLHSVSVCTLMIAFGRSLGLAGEDLRQAGIGGLLHDVGKMKVPDAVLNKPGRLTDA
                     EFALIKMHPGDGHAVLLETPGIGPVPLDITRHHHERLDGSGYPDGQQGEAISSMARMA
                     AIVDVYDAITADRCYHQGLPAAEALRKMWEWSAAHFDQKLLQAFMRCVGIYPVGSLVR
                     LESGRLGVVIDQNEGALLTPRVRVFFSTRSNAHIPPEIVDLGRKPGHGGGDRIVSAEA
                     PDKWGVDPARFLLREG"
     gene            3573..4397
                     /locus_tag="PLH85_10220"
     CDS             3573..4397
                     /locus_tag="PLH85_10220"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNB64983.1"
                     /translation="MDIILLVKALILGVVEGLTEFLPISSTGHLILAGDLLNFNDDRG
                     KLFEIVIQSGAILAVVWEFRERLARTFAGLGRQREANRLVLNVAIAFLPLAVLGLAFK
                     KVIEAHLFKPVPVALAFIVGAFVILWAERRDHRIRIHSVDEMRPLDALKLGIAQAFAL
                     IPGTSRSGATIIGGLLFGLSRKAATEFSFFLAIPTLFIATLYNLYKERALLNADDIGM
                     WVVGFIAAFVSAFLCVRWLLRYISHHDFVPFAWYRIFFGLVVLATWHFGLVEWTSS"
     gene            4660..5349
                     /locus_tag="PLH85_10225"
     CDS             4660..5349
                     /locus_tag="PLH85_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB64984.1"
                     /translation="MQHPGSDDSLLQHGSPLALRRAFKAAMNPERLAERADDLRAVLA
                     RLGHDPVLPDIHAPIGLAGHSFGAVTVQILAGERRPTQRVVPLDPRVRAILALSPSAR
                     GEGHDLTERFGALERPFFSLTGSRDDGMGLSDITAANRELPFRHMPPGHKYLLVFAGG
                     THLDFAGQSSPAEQGMFRLRRAPPPFQNALLAASTAFWRAHLLDDSDARHWLESGLPG
                     ILRNDDRYARK"
     gene            5346..5915
                     /locus_tag="PLH85_10230"
     CDS             5346..5915
                     /locus_tag="PLH85_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HNB64985.1"
                     /translation="MILYLHGFRSGPQSWKARSLAARMAELGLGDRLWCEQLPVSARA
                     TMALAEAAIAASPRPPTLVGSSLGGYYATYLAEKHGLRAVVVNPAVLAPLSLSAYLGP
                     QTHLYTGETFDFTPAHIDELRALDVPALSHPERFWLLAETGDEVLDYRHAVARYAGCR
                     QTVLDGGDHSFTRWDAYLDPILRFAGLIP"
     gene            5944..6786
                     /locus_tag="PLH85_10235"
     CDS             5944..6786
                     /locus_tag="PLH85_10235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB64986.1"
                     /translation="MSHPPGFDARRFKALERSGFNRIAARYADGAPLRADLQDALLAA
                     AALAPGESVLDLAAGPGLLARAAARQVLPGGCVVASDIAEGMLAEGLRRAADEGVPDL
                     LAAACDAEHLSFADARFDAALVGLGLFILPHPERAVAELHRVLRPGGRLAVSVWGPRN
                     DVPLIARALDCIARHLPAPKVPRPSVFRLGDAAVLHDLLDDAGFRAIAVTPHRLACRF
                     DSADAYWQAFLDLAGGAAEALLRLPDATQATLRAAVADDLAPHRDGAGFTLESTVLIA
                     TARR"
     gene            6858..9200
                     /locus_tag="PLH85_10240"
     CDS             6858..9200
                     /locus_tag="PLH85_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB64987.1"
                     /translation="MQGSWQVKSLVAALATIGIGSGLTGCGGGGSDVGTSTGVVVGSY
                     FQNAKVCLDENNNAVCDANEFSTRSDAQGRFTLPVAKVPVVAEIGTDASRLDPDTGTA
                     SAVTDKLVFRAPAGNTAVLSALTTLVVAEMDGGASLADARSTVAGRLGVDGAKLMGDY
                     NTETDSLTRSQLLAQGNNAIQTIAATVKTAGSDLKGGMGKTLTAKAVDSKVLYVWTQV
                     GADDTTPFSKVSITGSSSAVALNADPVKGKVADATTYTVSDSSNGSPVSAGALYARAI
                     TTAATCPSITVDGVAQPMAVRAPAITQVDSNGQYSTGSKSVPVKADFAVLTCEFRIPD
                     TARSAIVNGQALKLLNSSTALNRIVVIGDTGCRIKGPTAYTNGAGGDPLQDCTDDRAW
                     PYKRIAAAAASFNPDLVIHNGDIHYREGTPVGVETPAGVAGTGKNEDFLQQKGLTPTI
                     TYGWAAWEADFFKPTGALLSAAPWAITRGNHESCFRAATGWFHFLDPRPFPAATSAAK
                     EPKYSPTYDPSTCSDYIDPVAITLKDLQIVLLDNSGLQDDPGVGKNQGWTHGDHIRTA
                     RQLNALANLSASKDPTKVTWIVTHKPLFAYTGGTGAATPRTWQYQKAIAASGVNGVET
                     YAGGNGSLPANTQMLHAGHIHGWQMISHPAASNLPTSFLIGVSGDTLEAGFMFDSATG
                     APFAPANFGTAITSGVWPWLGAKAALWFGSLAQSLPDAFSTSPIVNANDPAKARITDF
                     GFVVFDRIPGTTNWTSTFYDMDRKKIRACTTVGKRTRCDG"
     gene            9281..9574
                     /locus_tag="PLH85_10245"
     CDS             9281..9574
                     /locus_tag="PLH85_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNB64988.1"
                     /translation="MFVVIVTYLRPLSEVDAAVPDHRAFLARHYAAGTFLLSGPQVPR
                     SGGVILARNVSRDALTALLAEDPFSQRGLAEYRIVEFAPNLAAADFAHLLPAD"
     gene            complement(9579..11021)
                     /locus_tag="PLH85_10250"
     CDS             complement(9579..11021)
                     /locus_tag="PLH85_10250"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNB64989.1"
                     /translation="MTAALVWFRRDLRDVDHAALYHALRRHDRVHCAFVFDTEILDAL
                     PGRMDRRVEFIHGSVVALDAALRARGGGLIVRHGRARDVIPALAASLGVAEVLSNRDY
                     EPAAIARDDEVAARLRGMGIGFADFKDQVIFERGEVLTQGGAPFSVFTPYKRAWLKAL
                     TPFYVKAYPVEAYAGHLAPPPDGTGVPTLAELGFATTNLAELGVTPGRAGGRRLLADF
                     LTRIGRYKSARDFPARKGVSYLSVHLRFGTVSIRELAAAALAVGGEGAETWLSELIWR
                     DFYQQILWHHPRVVAASFRPEYDRVVWDDAPDLFAAWCEARTGYPLVDAAMCQLLHSG
                     YMHNRLRMVVASFLTKDLGIDWRLGEAFFAEHLIDFDLAANNGGWQWAASTGCDAQPY
                     FRIFNPVTQSEKFDPDGAFIRRYLPALARVPARYLHAPWTMPPVDQKLAGCVIGQDYP
                     APVVDHARARARTLARFAVVKGADGDVAEA"
     gene            11158..11724
                     /locus_tag="PLH85_10255"
     CDS             11158..11724
                     /locus_tag="PLH85_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNB64990.1"
                     /translation="MTKPTINLTDHFLIAMPAMADPNFARTLTYIAEHNENGAMGVIV
                     NRPTDMRLATLMERIDIPFEQPAFADLPVYFGGPVQSDRGFVLHRPAGQWHSSLKVTD
                     DIGLTSSRDVLQALALDGEPAEVLVALGYAGWSAGQLEHELTQNAWLTVPADLTIVFD
                     LPPEERLAAAMQLLGVDFAKLSDVAGHA"
     gene            11721..12149
                     /gene="ruvX"
                     /locus_tag="PLH85_10260"
     CDS             11721..12149
                     /gene="ruvX"
                     /locus_tag="PLH85_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNB64991.1"
                     /translation="MSTVLGFDFGLARIGVAVGETETGLAHPLAVIGDEANAARFAAI
                     EKLLAEWKPATLVVGLPTHLDGSEHAMTARCRRFANQLHGRYGLPVALVDERLSSAEA
                     EARLKEAGHAGWRRQKPLLDATAAQIILSQYLENAAHAAP"
     gene            12136..12636
                     /gene="pyrR"
                     /locus_tag="PLH85_10265"
     CDS             12136..12636
                     /gene="pyrR"
                     /locus_tag="PLH85_10265"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNB64992.1"
                     /translation="MQLPDAEQLIATLAEAMRPQVSPDTALVGIHTGGVWLAQRLHAL
                     LGLSQPLGSIDVSFYRDDYAAKGLHPRPQGSKLPFDVEGTPVIIVDDVLYTGRTTRAA
                     LNELFDYGRPARVDLAVLVDRGGRELPIHPRFCALTLTEPLPSDQNLQLERDEAGALS
                     LRLIHA"
     gene            12629..13588
                     /locus_tag="PLH85_10270"
     CDS             12629..13588
                     /locus_tag="PLH85_10270"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNB64993.1"
                     /translation="MRNPQLNRNGELQHLLTLDGLPRAILTQILDTAEPFTEVAEREV
                     KKLPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNVATSSTKKGETLLDTID
                     NLCAMQADMFVVRHESSGAPYLIAQHLQTTGRDHIHVVNAGDGRHAHPTQGLLDMFTI
                     RHYKKEFHNLTVAIVGDVLHSRVARSQIAALTTLGVPEVRVIGPRTLIPTDVEKLGVR
                     YCPTMAEGLKDVDVVMMLRLQNERMNGALLPSPQEFFKIWGLTADKLALAKPDAIVMH
                     PGPMNRGVEIDSAVADGGQAVILPQVTFGIAVRMAVMSMLGSH"
     gene            13599..14882
                     /locus_tag="PLH85_10275"
     CDS             13599..14882
                     /locus_tag="PLH85_10275"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB64994.1"
                     /translation="MNLHIQNGRLIDPANRLDTHQDLFIADGKIVGVGSAPAGFSAGR
                     VIDARGLVVAPGLIDLAARLREPGFEYKATLESEMDAAMAGGITSLAIPPDTDPVLDE
                     PGLVEMLCYRAKKLNRAHVYPVGALTIGLKGQQLSEMAELVEAGCVAFSQANVPIVDT
                     RVLYRAMLYAATFGFRLWLQPIDPHLSRGGVAHDGEVASRLGLPGIPAAAETVALYTY
                     LQLARLTGARLHITRLSSADSLDHIEQARRDGVDVTCDVAAHALHLSDMDIGYFNPHC
                     HVIPPLRSQRDRAALSKAVAEGRINAICSDHTPVDDDAKQAPFSESEPGNTGLELLLP
                     LTLKWAGEHGLPLLDALARITSDAAKIAGIAKGGHLSVGARADVCVFDPEAYQLITRS
                     LLKSQGKNTPFLGLELPGKVRYTLVEGSLMYEAAA"
     gene            15351..16376
                     /locus_tag="PLH85_10280"
     CDS             15351..16376
                     /locus_tag="PLH85_10280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB64995.1"
                     /translation="MSELVEQAAERLRKAVPIMVKRGIPVTPVNYALWYTYVANENPH
                     FNQHIDDIVATYGTIPPAKAEGLFRDHVSPTGTQENALSKMKSGLERMVADLSRDLYS
                     TLEGTQNFNSLLDECNRGLLAPSGSGSLEEVLGTVERLIKGANAMQQHTSRFESSLST
                     ADEEIRRLRGELATLRKDALHDDLTGVFNRRAFDQELDQFIGQGNMGPSLHLGMLDID
                     HFKAFNDRFGHPVGDRVLQVVGAQLNEMAQVGVSVYRYGGEEFCLIFCGGTLAQAQEQ
                     ADRLRHTIEQLVLKDNRSGERLATITVSIGLASHVYGQSSITLVERADAALYEAKNSG
                     RNRVCIA"
     gene            complement(16389..17009)
                     /locus_tag="PLH85_10285"
     CDS             complement(16389..17009)
                     /locus_tag="PLH85_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB64996.1"
                     /translation="MRAGRARRRPQSGVVYLLTLFAVAALGLGLAQFGQVWAKSVQRE
                     REAELLFIGGEFARALARYKAAGPGVGPAELGDLVTDKRVPVPRRHLRRIYRDPMTGK
                     ADWIVERREGQIVGVRSRSEQSVLRRQGLPDWVQVIDVAQRTRGGFRQAPLSLLRGKE
                     ADTPSYTDWLFRPKEGPGAAPGAAGRPAAHSAAAPTPPAATAGAVP"
     gene            complement(17006..17392)
                     /locus_tag="PLH85_10290"
     CDS             complement(17006..17392)
                     /locus_tag="PLH85_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB64997.1"
                     /translation="MEPLSPRRRGFTLVELLVVMAIVAVLLSIAAPRYFGHLERAREA
                     ALTETLAVTRDAIDKFYGDTGRYPKDLDELVSRRYLRAVPVDPITERRDTWSPVTAPE
                     ALGGGMSDLHSGAEGKTRSGQAYEDL"
     gene            complement(17392..17865)
                     /locus_tag="PLH85_10295"
     CDS             complement(17392..17865)
                     /locus_tag="PLH85_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB64998.1"
                     /translation="MRRRRGFTLIEMAVTVAVVAVLAAIAAPVVQLSAQRAREAELRT
                     ALRQIREAIDTYKRYSDEGRIAKVVDASGYPPALEDLVKGVPDAKSPKGARLYILRRI
                     PRDPLNPDLSLAADETWGLRSYASPPDEPAEGADVFDVYSRATGKGLNGIAYREW"
     gene            complement(17874..20117)
                     /locus_tag="PLH85_10300"
     CDS             complement(17874..20117)
                     /locus_tag="PLH85_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspD"
                     /protein_id="HNB64999.1"
                     /translation="MTSRRLPRLLRRLAPLALTVLLAACAGNKALEESRRSFATQSPD
                     VALIDLRSRVEADPRNLELRAYYLRQREALTTRQLAMADQARGAGRFEEAENALALAR
                     QFDPNNPRVVAGVDAIAAQRQRNQLAAEADRRLAAGDFAGAEAAARTVLAGEPTHAAA
                     RAVMRRLDERAVARDPAPAALKGPLAKPVNLEFRDAPLRGVFEVLSRVSGLNFVMDRD
                     VRTDAKVTIFVRNNSIDDVLKLLMITQQLERKLLNDNSVLIYPNTPAKQRDYQELATR
                     SFYLANADTKQAMNLIKQVVKTKDVFIEEKLNLLVMKDTPEAIRLAERLIATLDLAEP
                     EVMLEVEVMEISRNKLLELGMRFPNQIGYGVLQPTVTNSVTTATGTTITQSLGGQLLQ
                     GNIDLRGGRSGVVPYVANPGLLLNLTDQDGNANILANPRIRVKNRDKAKIHIGEKLPI
                     FTTTATANVGVSASVNYLDVGLKLEVEPSVHMDDDVAIKVNLEVSSVVKEVTGPSNSI
                     AYQVGTRSASTSLRLKDGETQVLAGLISDEERSSAARLPGLGEVPALGRLFSSQRDVE
                     NKTEIILLITPRVVRNVTKPGLASATQAAGTEAAVGVSALSIGPTAPGALGVAAAAPG
                     AMPAASPATGALAAATSVQAPPEVKAGEEFTVSVGVQAGGNEGGSVDLNFDSRLVESA
                     DGPADGDLRKLTLPPGTAGVVFARFRVRPGASGTAVFALGQAQLRVGGQAQMVPPPPP
                     VSVQIQP"
     gene            complement(20114..20608)
                     /locus_tag="PLH85_10305"
     CDS             complement(20114..20608)
                     /locus_tag="PLH85_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65000.1"
                     /translation="MKRVHGLLAGLALTLLAAWWAAGLEDEPATEAPRRAVQRKAASV
                     RSAPRVDLAALAASRPALADGDAGAPVPDLFPVRSFAPPPPPPPPPPTPTAPPLPFRY
                     TGMLEEGGRHAAFLEQGEQIRVVRVGDTLDGRYRVTAVSRARIDLIYLPLNEPQSLVT
                     GAMP"
     gene            complement(20605..21174)
                     /locus_tag="PLH85_10310"
     CDS             complement(20605..21174)
                     /locus_tag="PLH85_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65001.1"
                     /translation="MSLRLTGARWATLHESAVRTFRAAGWAGVVGVALTVFAVCFDLS
                     GNRALEAQADALEQETRSLRQRLRTHPEGVAVPERTRLERFYGAFPATDALPDLLVRL
                     HGYALARGLKLDRADYRSAPEGGTPLERVTLELPARGPYPVLRTWLADVLAEMPEVAL
                     DGLSLRRASMATPEVEAQVRFVVFLRRQP"
     gene            complement(21171..21716)
                     /locus_tag="PLH85_10315"
     CDS             complement(21171..21716)
                     /locus_tag="PLH85_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65002.1"
                     /translation="MSPHRAPPPLGLDFVGGLRRPGWLGWAVLAFGVIAAVGVLHEYD
                     VLETRVAEAAHALTRTKHGLDLRASGVDRAPVTEQEARPARAVAQLLQRDWRAVLGVL
                     ETASDDPGIALLALDQEGARGGLRLTGEGRSLPEVFAYVKRLEAGGVLREVRLGSYEF
                     HASGPLQVVGFTVTARWEVAP"
     gene            complement(21713..>22245)
                     /locus_tag="PLH85_10320"
     CDS             complement(21713..>22245)
                     /locus_tag="PLH85_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65003.1"
                     /translation="LRIDWPAGLRAAERAAFTRHRFQTVFGIGEDWCVLADRNAVVLP
                     SLACALPRALLEAVQDFARTRHLRLVRLEGAFAAEFNRHGRRFIGDGAFARAEAGRVT
                     VALWRDGVWAAVRSQAVAVADAAAAAGCLAGLLPGLAVAGQAMAQGTLYLAGAAATAT
                     ALPAGWTLAGVEGGAS"
BASE COUNT         3551 a         8563 c         6908 g         3223 t
ORIGIN      
        1 taaggctgta acagacgctc agagcggccg cttgaccgtc agggcacccc ggatctggcc
       61 cttggtatag cccgtcgccc ggtcatcggg ataatggcgg gcgagttgct cccgcaaggg
      121 cgcgtccagg gtgtccgcgg ccccgtggca ggccagacac acgtcgcccg ccggaatggc
      181 ccggatgtag cggtagaccg gcttgccgtg ctcgctgacg atctcgccgg tttcgagggt
      241 ctcgaccttc tcgccattgg cagcgcggat gttgaagtcg gcgagctggc gcgcctccca
      301 tacgtctggc gtgccgcgct ggggattgcg cgtcttcagg ctgacccggc ggatgtccca
      361 gccggtttcg gccgagagcg cccggaggag ttgcggggcc ttttccttgc acacggggat
      421 ggcggcgacc gtaccttcgg cgacgacggt ctccttcatc atgcccgcca ccttgggcag
      481 cacgggcagg gccttctggc gggtttcgga tttgagggcg tccaggtcgg cggcctgggc
      541 gatgcccgca ctcagggcga gggcagcgat gaacatgtga cgcatcggaa catcctaatc
      601 ctaaattaat cagtacatac taatatactt tatttctgcc ccgcgcggca cgtccccgcc
      661 acagccgtac cccatgtgcg accagaccag ccgccgcggt ggcggccaga agtccctctt
      721 cggcccgccg caggtccggc agcagccgct ccatcagggc gctgaagtaa tagccgccgc
      781 cgccgatcac cgccacccag agcgcggccc cgagaaggtt gagaagcacg aagcgcaagg
      841 cctgcacccg actccagccc atcagcacgg gcccggcgat gcgcaggccg atcatgaagc
      901 ggatgcccag aacgaggagg ttctgatgcc gcaggatcag gtcctggatg cggggaacgg
      961 cccgctgcac cgctgcgaat cgccggatca gccggtctcc gaagcgccgc ccgacaaaaa
     1021 accaggcctg atcaccggcg aatcccgcca caaacgccac cgccgccacg gcgggcagat
     1081 cgagaagacc ccgatgcacc gccacaccgg ccagcagcag cacggtttcg ccttccagca
     1141 ggcagcccac cgccacggcg aaatagccgt aatgggccac ccagtccgcg agactcatcc
     1201 caggcggcgg ataaacttgg tgaacgccaa cagttgttcg tccaggcgct tgagggtgcc
     1261 ggtgtccgca agttcgcccg cggcgttcac cttgtcctgt accgcaccga tcatgacctc
     1321 aggcttgttg aggacgtggc agtccaggaa gaccagcacc tggcgcaggt gatactggca
     1381 gcgggcaccg cccaggatgc ccatggacgc agtctggatc gccaccggct tgccggcgaa
     1441 gggctgctcc ggcacgcggg acagccagtc gatggcgttc ttgagggcgc cgggcacgga
     1501 atagttgtat tcgggcgtga cgatcaccac cccgtcggcc gcgcggatcg cgtccgccag
     1561 ggcaatgacg gcaggcggaa agccctccgc ctgcaggtcg gcgtcgtaca cggggatgtc
     1621 gcggatcgag ggcagcgcct cgaccaccac gtcctccccc gccacggtcg gcagggcccg
     1681 ggcgatggcc gcgttgtagg agccctggcg caggctgccg agcagggtga cgattttgag
     1741 cggtgacgac atggcggact ccttctgggg gatggggagc cccattgtcc cagagctgcg
     1801 ccggcaatgt caggctccgg cggaatatcc cggaaaccat tccgccgacc cgttgcccaa
     1861 accctgggtg caaaaatcct gtcatgcccg cccccacgaa aaggagaacg ccatgcaagc
     1921 cccgactcat accctcggcc acctgttcga acagctgggc ctgcccgccg acaccggcgc
     1981 catcgaagcc ttcctcgaca gccacagcct gccgcccgac atgcgtctgg ccgacgcgcc
     2041 cttctggacg aagccccagg ccgacttcct ggcccgcgag ctggccgacg acgccgactg
     2101 ggccgaactg atcgacagcc tcgacgcgcg cctgcggcac tgacgccgga tcacccctcc
     2161 cgcagcagga agcgcgccgg atccacgccc catttgtccg gcgcctcggc ggagacgatc
     2221 cggtcgcctc caccgtggcc cggcttgcga ccgagatcca cgatctcggg cgggatgtgg
     2281 gcgtttgagc gggtactgaa gaacacccgc acccgcggcg tgagcagcgc accctcgttc
     2341 tggtcgatca ccaccccgag gcggcccgat tccaggcgta ccaggctacc caccggataa
     2401 atccccacgc agcgcatgaa ggcctgcagc agcttctgat cgaagtgggc cgcgctccat
     2461 tcccacatct tgcgcagggc ttccgccgcc ggcagaccct ggtggtagca gcggtcggcg
     2521 gtgatcgcgt cgtagacatc cacgatggcc gccatgcgcg ccatgctgct gatggcctcg
     2581 ccctgctggc cgtccgggta gccactgccg tccaggcgct cgtggtgatg ccgggtgatg
     2641 tccagaggca ccgggccgat gcctggcgtc tccagaagga ccgcgtggcc gtcgcccggg
     2701 tgcatcttga tgagggcgaa ctccgcgtcg gtgagccgcc ccggcttgtt caggaccgca
     2761 tcggggacct tcatcttgcc cacgtcatgg agcagcccgc cgatcccggc ctggcgcaga
     2821 tcctcgccgg ccagcccgag cgaacggccg aaggcgatca tgagcgtgca tacgcttacc
     2881 gaatgcagaa aggtgtagtc gtccttgttc ttgatgccga tcagcccgat cagcgcgccg
     2941 ctgttgcgca gcaccgagcc ggtgatggcc tccaccaccg gttccgcgtc ctccagggac
     3001 acggcccgcc cgaggcgcac gtcctgcatc atccctcgca ccacccggtg ggcctgttcg
     3061 tgcacacgcc gcgcccgctc catctcctcc gccaccgaca cccgcagggg cgtctcctcc
     3121 ggcgtcgcca gagccgccac catctgggct tcgacggcct cgcgcacggc accggcatcc
     3181 accgcctccg cgtcgtcgag acccttgtcc gtgtcgatat agacatggcg gatgccggac
     3241 tcgacgatgc gctgcaggtc gtcaccgcca ttcagcctga aacgcgcacg ccagaatggg
     3301 tgggacatcc agtcgccgca cagctcgtgg atgaacatgc cggctctgag ccgctctacg
     3361 gggatcttct tcaacatcgc acggggccaa tggatcgcat ccagagtggt atcggccgcc
     3421 cggccccgac ctgaagcggt atgccgttga actgagcacc ccgtcacggc ggcgtgcggg
     3481 aagttcgtgt ccgagcgtcg ccggcccgtc taaggcgtca tggagcaagg ctataatgcg
     3541 cgctcttttt gcactgcaca gtcaacccgt ccatggacat catcctgctc gtcaaggccc
     3601 ttatcctggg cgtcgtcgaa gggctcaccg agtttctgcc catctccagc accggccacc
     3661 tgatccttgc cggcgacctc ctcaacttca acgacgaccg cggcaagctc ttcgagatcg
     3721 tcatccagtc cggtgccatc ctcgccgtgg tgtgggaatt ccgcgagcgc ctcgcccgca
     3781 ccttcgccgg cctcggccgc cagcgggaag ccaaccgcct ggtgctcaac gtggccatcg
     3841 cattcctgcc cctggccgtg ctcgggctgg ccttcaagaa ggtcatcgaa gcccacctct
     3901 tcaaaccggt gccggtggcc ctcgccttca tcgtgggcgc cttcgtcatc ctgtgggccg
     3961 agcgccgcga ccatcgcatc cgcatccact ccgtggacga gatgcgcccg ctcgacgccc
     4021 tcaagctcgg catcgcccag gccttcgcgc tgattcccgg cacctcgcgc tccggcgcca
     4081 ccatcatcgg cggcctgctc ttcggcctgt cgcgcaaggc ggccaccgag ttctccttct
     4141 tcctggccat ccccaccctg ttcatcgcca ccctgtacaa cctgtacaag gagcgcgccc
     4201 tgctcaacgc cgacgacatc ggcatgtggg tcgtcggctt catcgccgcc ttcgtctcgg
     4261 ccttcctgtg cgtgcgctgg ctgctgcgct acatctccca ccacgacttc gtgcccttcg
     4321 cctggtaccg catcttcttc gggctggtgg tgctggcgac ctggcatttc ggtctggtgg
     4381 aatggaccag cagctgaggg cccgcccctg agcctgcctc ccgacccgga cgcgcccttc
     4441 gcccccgcgg acggccgccc gggcccccac ccggtcgtca cgcagctcga cacgctgcac
     4501 gacgaacacc gcgcacgcag cctgccgctc aagcgctacc tgccgcaggg cgttgcgccg
     4561 gatggtctga tcctcttctc ccacggcctc ggcggctccc gcgaaggtgg cgctgactgg
     4621 ctcgcccact gggcttcgtg gggattcgcc ggtctcgcgg tgcagcatcc cggcagcgac
     4681 gacagcctcc tgcagcacgg ctcgcccctc gccctgcgcc gcgccttcaa ggcggcgatg
     4741 aatcccgaac gcctcgccga gcgggccgac gacctgcgcg ccgtcctcgc ccgcctcggc
     4801 cacgaccccg tcctgccgga catccatgcg cccatcggcc tggccggtca ctccttcggc
     4861 gccgtcaccg tacagatcct ggccggcgaa cgccgcccga cccagcgtgt cgtccccctc
     4921 gatccccgcg tgcgggcgat cctcgccctc agcccctcgg cgcggggcga gggccacgat
     4981 ctgaccgagc gcttcggcgc cctcgaacgc ccgtttttca gcctcaccgg cagccgcgac
     5041 gacggcatgg gcctgtccga catcaccgcc gccaaccggg agctgccctt ccgccacatg
     5101 ccgccgggcc acaaatacct gctggtcttt gccggcggca cccacctgga tttcgccggc
     5161 cagtccagcc ccgccgaaca aggcatgttc cgcctgcgcc gcgccccgcc acccttccag
     5221 aatgcgctcc tggccgccag caccgccttc tggcgcgccc acctcctcga cgacagcgac
     5281 gcccgccact ggctggaaag cggcctcccc ggcatactcc gcaacgacga ccgctacgcc
     5341 cgcaaatgat cctctacctc cacggctttc gttccggccc tcaatcctgg aaagcccgca
     5401 gcctcgcggc gcgcatggcc gaactcggcc tcggcgaccg cctctggtgc gaacagcttc
     5461 cggtgtcggc ccgcgccacc atggccctgg ccgaagcggc catcgccgcc agcccgcgcc
     5521 cacccaccct ggtgggcagt tccctcggcg gctactacgc cacctacctg gcggaaaagc
     5581 acggcctgcg tgccgtggtg gtcaatccgg cagtcctcgc gcccctctcc ctgagcgcct
     5641 acctgggccc ccagacccac ctctacacgg gcgagacctt cgacttcacg ccagcccaca
     5701 tcgacgagct gcgcgccctc gacgtgcccg ccctgagcca cccggagcgc ttctggctgc
     5761 tcgccgagac cggcgatgag gtgctcgact accgccacgc cgtcgcccgc tatgccggct
     5821 gccgccagac cgtgctggac ggtggcgacc acagcttcac ccgctgggac gcctacctgg
     5881 acccgatcct gcgcttcgcc ggcctgatcc cctgatccgc gcccacgcac cccacgcccg
     5941 cccatgtccc atccgcccgg tttcgacgcc cgccgcttca aggccctgga acgcagcggc
     6001 ttcaaccgca tcgccgcccg ctacgccgac ggcgcccccc tgcgcgccga cctgcaggac
     6061 gccctgctcg ccgccgccgc cctcgcgccc ggcgagagcg tcctcgatct ggccgccggg
     6121 ccgggcctgc tcgcccgcgc cgcggcccgc caggtgctgc ccggcggttg cgtcgtggcc
     6181 agcgacatcg ccgaaggcat gctcgccgaa ggcctgcgcc gcgccgccga cgaaggcgtg
     6241 ccggacctcc tcgccgccgc ctgcgacgcc gagcacctga gctttgccga cgcccgcttc
     6301 gacgcggccc tggtcggcct cggcctcttc atcctgcccc acccggaacg ggcagtggcc
     6361 gaactgcacc gcgtgctgcg ccccggcgga cgcctcgccg tgtccgtgtg gggcccgcgc
     6421 aacgacgttc ccctcatcgc ccgtgccctc gactgcatcg cccgccatct gcccgcgccc
     6481 aaggtgccgc ggccttcggt gttccgcctc ggcgacgcag ccgtcctgca cgacctgctg
     6541 gacgacgccg gcttccgcgc catcgccgtg acgccccacc gcctggcctg ccgcttcgac
     6601 tccgccgacg cctactggca ggccttcctc gatctggcgg gcggcgccgc cgaagccctg
     6661 ctgcgcctcc ccgacgccac ccaggcgacc ctgcgcgccg ccgtcgccga cgatctcgcg
     6721 ccccatcgcg acggcgccgg cttcacgctg gaaagcaccg tcctcatcgc cacggcccgc
     6781 cgctgagccc gccccgtcac gacgctgtta cacacattcc ctacccttct gcgctttcag
     6841 atgcgaagga aacgggcatg caaggctcct ggcaggtcaa atccctggtc gccgccctgg
     6901 cgacgatcgg aatcggcagc ggactgacgg gctgcggcgg cggtggatcg gacgtcggca
     6961 ccagcaccgg cgtggtagtc ggcagctact ttcagaacgc caaggtctgt ctggacgaga
     7021 acaacaacgc cgtctgcgac gccaacgagt tctccacccg cagcgacgcc cagggccgct
     7081 tcacgttgcc cgtggccaag gtgccggtgg tcgccgagat cggcaccgac gcctcccggc
     7141 tcgatcccga caccggcacc gcttcggctg tcaccgacaa gctcgtgttc cgcgcccccg
     7201 ccggcaacac cgccgtgctg agcgccctca ccaccctggt cgtcgccgag atggacggcg
     7261 gcgccagcct ggccgacgcc cgcagcaccg tcgccggccg cctcggggtg gatggcgcca
     7321 agctgatggg cgactacaac accgaaaccg acagcctcac caggagccag ctcctcgccc
     7381 agggcaacaa tgccatccag accatcgccg ccaccgtcaa gaccgccggc agcgacctga
     7441 agggcggcat gggcaagacc ctcaccgcca aggccgtgga cagcaaggtg ctgtacgtct
     7501 ggacccaggt gggtgccgac gacaccacgc ccttcagcaa ggtgagcatc accggcagca
     7561 gcagcgccgt cgccctcaat gccgatccgg tcaagggcaa ggtcgccgac gccaccacct
     7621 acaccgtcag cgacagctcc aacggcagcc cggtcagcgc cggcgccctc tacgcccgcg
     7681 ccatcaccac cgccgccacc tgcccgtcca tcaccgtgga tggcgtcgcc cagcccatgg
     7741 ccgtgcgtgc gccggccatc acccaggtgg acagcaacgg ccagtactcc accggcagca
     7801 agagcgtccc cgtcaaggcc gacttcgccg tgctcacctg cgaattccgc atccccgaca
     7861 ccgccaggtc cgccatcgtc aatggccagg ccctcaagct cctcaacagc agcaccgccc
     7921 tcaaccgcat cgtggtgatc ggcgacaccg gctgtcgcat caagggcccc accgcctaca
     7981 ccaacggcgc cggcggcgac ccgctgcagg actgcaccga cgaccgcgcc tggccctaca
     8041 agcgcatcgc cgccgccgcg gccagcttca acccggatct cgtcatccac aacggcgaca
     8101 tccactaccg ggaaggcacg cccgtcggcg tcgagacccc cgccggcgtg gccggcaccg
     8161 gcaagaacga ggacttcctg cagcagaagg gcctgacgcc caccatcacc tacggctggg
     8221 ccgcctggga agccgacttc ttcaaaccca ccggcgccct cctcagtgcc gcgccgtggg
     8281 ccatcacccg cggcaaccac gaatcctgtt ttcgcgccgc caccggctgg ttccacttcc
     8341 tggatccgcg ccccttcccg gccgccacca gcgcggccaa ggagcccaag tactcgccca
     8401 cctacgaccc cagcacctgc agcgactaca tcgacccggt ggccatcacc ctcaaggatc
     8461 tgcagatcgt gctgctcgac aacagcggcc tgcaggacga tccgggcgtc ggcaagaacc
     8521 agggctggac ccacggcgac cacatccgca ccgcccgcca gctcaacgcc ctggccaacc
     8581 tgagcgcctc caaggacccc accaaggtca cctggatcgt cacccacaag cccctgttcg
     8641 cctacaccgg cggcaccggc gcggccaccc cgcgcacctg gcagtaccag aaggccatcg
     8701 ccgccagcgg cgtgaacggc gtcgagacct acgccggcgg caacggcagc ctgcccgcca
     8761 acacccagat gctccatgcc ggccacatcc acggctggca gatgatcagc catccggcgg
     8821 cgagcaacct gcccacctcc ttcctgatcg gcgtctccgg cgacaccctg gaagcgggct
     8881 tcatgttcga cagtgccaca ggcgcaccct tcgcgccggc caacttcggc acggccatca
     8941 cttcgggcgt gtggccctgg ctgggcgcca aggcggccct gtggttcggc agcctggccc
     9001 agtccctgcc ggacgccttc agcaccagcc ccatcgtgaa tgccaacgac ccggccaagg
     9061 ccaggattac cgactttggc ttcgtggtct tcgaccgcat ccccggcacc accaactgga
     9121 cgagcacctt ctacgacatg gaccgcaaga agatccgcgc ctgcaccacc gtcggcaaac
     9181 gcacccgctg cgacggctga tcggcacgac gcccggcgga aatccgggca tcatggcggc
     9241 ccggccccat gccgggccgt tttcattctg cggagccccc atgttcgtcg tcatcgtcac
     9301 ctatctgcgc cccctctccg aggtggacgc cgccgtcccc gatcaccgcg ccttcctggc
     9361 ccgccactat gccgccggca ccttcctgct ctccgggccc caggtgcccc gcagcggtgg
     9421 cgtgatcctc gcccgcaacg tgagccgcga cgcgctcacc gccctgctgg ccgaagaccc
     9481 tttcagccag cgcggcctgg cggaataccg catcgtcgaa ttcgccccca acctggccgc
     9541 ggcggacttc gcccacctgc tgccggcgga ctgagatctc acgcctcggc gacgtccccg
     9601 tccgcaccct tcacgacggc aaagcgcgcc agcgtgcgcg cccgcgcgcg ggcgtgatcc
     9661 accaccggcg caggatagtc ctgcccgatc acgcagcccg cgagcttctg gtccacgggc
     9721 ggcatcgtcc acggggcgtg caggtagcgc gccggcaccc gcgccaacgc tggcaggtag
     9781 cggcggatga aggcaccatc cgggtcaaac ttctcggact gggtgaccgg attgaagatg
     9841 cggaaatagg gttgcgcgtc gcagcccgtg gacgccgccc attgccagcc gccgttgttg
     9901 gcggccaggt cgaaatcaat caggtgctcg gcaaagaagg cctcgcccag gcgccagtcg
     9961 atgcccaggt ccttggtcag gaaagacgcc accaccatgc gcaggcggtt gtgcatgtag
    10021 ccgctgtgca ggagctggca catcgccgca tcgacgaggg gatagccggt gcgggcctcg
    10081 caccaggcgg cgaacaggtc cggcgcgtcg tcccacacca cccggtcgta ctccggacgg
    10141 aagctggcgg ccacgacgcg cgggtgatgc cacaggatct gctggtagaa atcgcgccag
    10201 atcagctccg acagccaggt ctcggcgccc tcgcccccca ccgccagcgc ggcggcggcc
    10261 agctcgcgga tcgacacggt gccgaagcgc agatgcaccg acaggtagga cacgcccttg
    10321 cgcgccggaa aatcccgcgc cgacttgtaa cgcccgatgc gcgtaaggaa atccgccagc
    10381 agccggcgtc cgcccgcgcg gcccggcgtc acgcccagtt ccgccaggtt ggtggtcgca
    10441 aagcccagct cggccagggt cggcaccccg gtcccgtccg gcggcggcgc caggtggccc
    10501 gcatacgcct ccaccggata ggccttgacg taaaagggcg tcagcgcctt cagccaggcc
    10561 cgcttgtaag gcgtgaacac cgagaacggc gccccgccct gagtcagcac ctcgccgcgc
    10621 tcgaagatca cctgatcctt gaagtccgca aagccgatgc ccatgccccg cagacgcgcc
    10681 gccacctcgt cgtcccgggc gatggctgcc ggctcgtagt cccggttgct cagtacctct
    10741 gcaacgccca gggacgctgc aagcgccggg atcacgtcgc gggcgcgccc gtggcgcacg
    10801 atcagtccgc ccccccgcgc ccgcagggcc gcgtccagcg ccaccacgct gccgtggatg
    10861 aattccacgc gccggtccat tcggcccggc agcgcgtcga ggatctcggt gtcaaagacg
    10921 aaggcgcaat gcacgcgatc gtggcggcgc agggcgtggt acagggcggc gtggtccaca
    10981 tcgcgcagat cgcggcggaa ccacacgagg gcggcagtca tcggacactc ccggcagacg
    11041 gagccggcat cctaacgagc cgcgcggcac gcggcaaacc ccgacggcgc aaccgtcccc
    11101 acggctttcc gcagcgtgca ggaacaattc cgccgttctc ggcttaaaat agacagtgtg
    11161 acgaaaccga ccatcaatct caccgaccat ttcctgatcg ccatgcccgc catggccgat
    11221 ccgaactttg cgcggaccct cacctacatc gccgaacaca acgagaacgg cgccatgggg
    11281 gtgatcgtca accggcccac ggacatgcgc ctggccacgc tgatggagcg catcgacatc
    11341 cccttcgagc agcctgcctt tgccgacctg ccggtgtatt tcggcggccc ggtgcagtcc
    11401 gaccggggct tcgtgctgca tcgcccggcc ggccagtggc attccagcct caaggtcacg
    11461 gacgacatcg gcctcaccag ctcccgcgac gtgctccagg ccctcgccct cgacggcgag
    11521 cccgccgagg tgctggtcgc cctcggctac gccggctggt ccgccggaca gctcgaacac
    11581 gaactcaccc agaacgcctg gctcaccgtg ccagccgatc tgaccatcgt cttcgacctg
    11641 ccgccggaag agcgcctcgc cgccgccatg cagctgctcg gcgtggattt cgccaagctc
    11701 tcggacgtcg ccggccacgc gtgagtaccg tcctcggctt tgacttcggg ctggcccgca
    11761 tcggcgtggc ggtgggtgaa acggaaaccg gcctggccca tcccctcgcg gtgattggtg
    11821 acgaagccaa cgctgcgcgc tttgccgcca tcgaaaaact cctcgccgaa tggaagccgg
    11881 ccaccctggt ggtcggcctg cccacccacc tggacggcag cgaacacgcc atgaccgccc
    11941 gctgccggcg cttcgccaac cagctccacg gccgctacgg cctgccggtg gccctggtgg
    12001 acgagcggct cagctccgcc gaggccgaag cgcgcctcaa ggaagccggc catgccggct
    12061 ggcgccggca gaaacccctg ctcgacgcca ctgccgccca gatcatcctg tcccagtacc
    12121 tggagaacgc cgcccatgca gctccctgac gccgaacagc tcatcgccac cctggccgag
    12181 gcgatgcgcc cccaggtctc gcccgacacc gccctggtgg gcatccacac cggcggcgtg
    12241 tggctcgccc agcgcctgca cgccctgctc ggcctgtccc agcccctggg cagcatcgac
    12301 gtgagcttct accgggacga ctacgccgcc aagggcctgc acccgcgtcc ccagggctcc
    12361 aagctgccct tcgacgtgga gggcacgccg gtgatcatcg tggacgacgt gctctacacc
    12421 ggccgcacca cccgcgcggc cctcaacgaa ctcttcgact acggccgccc ggcgcgcgtc
    12481 gacctggccg tgctggtgga ccggggcggc cgcgaactgc ccatccaccc gcgcttctgc
    12541 gccctcaccc tgaccgagcc gctgccgtcc gaccagaacc tgcaactgga gcgcgacgaa
    12601 gccggcgccc tctccctgag gctgatccat gcgtaatccc caactcaacc gcaacggcga
    12661 actgcagcac ctgctcaccc tcgacggcct gccccgcgcc atcctcaccc agattctgga
    12721 caccgccgag cccttcaccg aagtggccga gcgggaagtg aagaagctcc ccctgctgcg
    12781 cggcaagagc gtcttcaacc tgttcttcga gaacagcacg cgcacccgca ccaccttcga
    12841 gatcgctgcc aagcgcctgt ccgctgacgt catcaacctc aacgtcgcca cctcgtccac
    12901 caaaaagggc gagaccctgc tggacaccat cgacaacctc tgcgccatgc aggccgacat
    12961 gttcgtggtg cgccacgaat cctctggcgc cccctacctc atcgcccagc acctgcagac
    13021 caccggacgc gaccacatcc acgtggtcaa cgccggcgac ggccgccatg cccaccccac
    13081 ccagggcctg ctggacatgt tcacgatccg gcactacaag aaggaattcc acaacctcac
    13141 cgtcgccatc gtcggcgacg tgctccacag ccgcgtcgcg cgctcccaga tcgccgccct
    13201 gaccaccctg ggcgtgcccg aagtgcgcgt catcggcccc cgcaccctga tccccaccga
    13261 cgtggaaaag ctcggcgtgc gctactgtcc gaccatggcg gaaggcctca aggacgtgga
    13321 cgtggtgatg atgctgcgcc tgcagaacga gcgcatgaac ggcgccctgc tgcccagccc
    13381 gcaggaattc ttcaagatct ggggcctcac cgccgacaag ctggccctcg ccaagcccga
    13441 cgccatcgtc atgcaccccg gcccgatgaa ccgcggcgtc gagatcgact ccgctgtggc
    13501 cgacggcggg caggcggtga tcctgcccca ggtcaccttt ggcatcgcgg tgcgcatggc
    13561 cgtgatgagc atgctgggca gccactgaga ccaccacgat gaacctgcac atccagaacg
    13621 gccgcctcat cgacccggcc aaccgcctcg acacccacca ggacctcttc atcgccgacg
    13681 gcaagatcgt cggcgtcggc agcgcgcccg ccggcttcag cgccggccgc gtcatcgacg
    13741 cccgcggact ggtggtcgcc cccggcctga tcgacctggc cgcgcgcctg cgcgaacccg
    13801 gcttcgaata caaggccacc ctcgaatccg aaatggacgc ggccatggcc ggcggcatca
    13861 ccagcctcgc catcccgccc gacaccgacc cggtgctgga cgaacccggc ctcgtcgaga
    13921 tgctctgcta tcgggccaag aagctcaacc gcgcccacgt gtacccggtc ggcgccctca
    13981 ccatcggcct caagggacag cagctctccg agatggccga gctggtcgaa gccggctgcg
    14041 tcgccttcag ccaggccaac gtgcccatcg tggacacccg cgtgctctac cgcgccatgc
    14101 tctacgccgc caccttcggc ttccgcctgt ggctgcagcc cattgacccg cacctgtccc
    14161 gcggtggcgt cgcccacgac ggcgaggtcg cctcgcgcct cggtttgccg ggcatccccg
    14221 ccgccgccga gaccgtggcc ctgtacacct acctgcagct cgcccgcctc accggcgccc
    14281 gcctgcacat cacccgcctg tccagcgccg acagcctcga ccacatcgag caggcccggc
    14341 gggacggcgt ggacgtcacc tgcgacgtgg ccgcccatgc cctgcacctg agcgacatgg
    14401 acatcggcta cttcaacccc cactgccacg tgatcccgcc cctgcgcagc cagcgcgacc
    14461 gggcggcctt gtccaaggcg gtggcggaag gccgcatcaa cgccatctgc tccgaccaca
    14521 cgccggtgga cgacgacgcc aagcaggccc ccttcagcga atccgaaccg ggcaacaccg
    14581 gcctcgaact gctcctgccc ctcaccctca agtgggccgg cgaacacgga ctgccgctgc
    14641 tggatgccct ggcccgcatc acctcggacg cggcaaaaat tgccggtatc gccaaaggcg
    14701 ggcacctgtc cgtaggcgcc cgcgccgacg tgtgcgtctt cgatccggag gcctaccagc
    14761 tcatcacccg cagtctcctc aagagccagg gcaaaaacac ccccttcctg ggcctcgaac
    14821 tgcccggcaa ggtgcgctac accctggtgg aaggcagcct gatgtacgaa gcggccgcct
    14881 gatccggccc aacccatccc atgttccaga cggcgcccgc gggcgccgtc gctttttcgc
    14941 cccgccgaac ccacgccccc gcccccgaag gacgtgccga ctcggcgcgg tccccacgca
    15001 ttgcgcagcg gcctgaccgc caaaatcctc cgaatccgcc aagccctcga aaaatgcatc
    15061 attcatcaaa atcccattcg cctaagctat atccaaagcc gtaggcaccc ccgccggaaa
    15121 gtgacagtac gcatttgttt tttatagatt ttttctcact gaaaaacgcc ttcttccgac
    15181 gtctccaatt aattcacaca ttgttgcaga acccgctcaa cttctccccc ggtgaccgat
    15241 atttcctgat gtcagccggg cgagcgccga tcggtggtcg tactggcgga ccggacctcg
    15301 tctctttggg ggaggtccgc gggagtccag aacacaaggg aagaacaaga atgagtgagc
    15361 tcgtcgaaca ggcagccgaa cgcctccgca aggccgtgcc catcatggtg aagcgcggca
    15421 tccccgtcac gccggtgaat tacgccctct ggtacaccta cgtggccaac gaaaaccccc
    15481 acttcaacca gcacatcgac gacatcgtcg ccacctacgg caccattccg ccggccaagg
    15541 ccgaaggcct gttccgcgat catgtctcac cgacaggcac ccaggagaac gccctctcca
    15601 agatgaagtc cggcctcgag cgcatggtgg ccgacctcag ccgcgacctc tactccacgc
    15661 tggaaggcac ccagaatttc aattccctgc tcgacgaatg caaccggggg ctgctcgcgc
    15721 cctccggatc cggcagcctg gaagaggtgc tggggacggt ggagcgcctc atcaagggcg
    15781 ccaacgcgat gcagcagcac acctcgcgtt tcgagagctc cctgagcacc gccgacgagg
    15841 agatccggcg tctgcgcggc gagctcgcca ccctgcgcaa ggacgccctc cacgacgacc
    15901 tcaccggcgt cttcaaccgc cgcgccttcg accaggaact ggaccagttc atcggccagg
    15961 gcaacatggg accgtcgctg cacctgggca tgctggacat tgaccacttc aaggccttca
    16021 acgaccgttt cggccacccg gtgggtgacc gcgtcctcca ggtggtgggc gcccagctca
    16081 atgagatggc ccaggtgggc gtgtcggtgt atcgctacgg cggcgaggag ttctgcctga
    16141 tcttctgcgg cggcaccctg gcccaggccc aggaacaggc cgaccggctg cgccacacca
    16201 tcgagcagct ggtgctcaag gacaaccggt ccggcgagcg gctggccacc atcacggtgt
    16261 ccatcgggct ggccagccac gtctacgggc agtcctcgat taccctggtg gaacgggccg
    16321 acgcggccct gtatgaggcg aagaacagcg gccgcaaccg ggtctgcatc gcctgacgcg
    16381 acgccgcgtc agggcacggc gccggcggtc gcggccggcg gcgtcggcgc tgcggccgaa
    16441 tgggctgccg gacgacccgc tgcacccggc gcagcgcccg gcccttcctt ggggcgaaac
    16501 agccagtcgg tgtagcttgg cgtgtccgcc tccttgccgc gcagcagact caggggtgcc
    16561 tggcggaagc cgccccgcgt ccgctgcgcc acgtcgatga cctggaccca gtcgggcagc
    16621 ccctgccggc gcagcacgga ctgctccgag cggctgcgca cgcccacgat ctgcccctcg
    16681 cgccgctcca caatccagtc cgccttgccc gtcatgggat cgcggtagat ccgccgcaga
    16741 tggcgacgcg gtacgggcac ccgcttgtcc gtcaccagat cgcccagctc cgcgggcccc
    16801 acgccggggc cggccgcctt gtagcgcgcc agggcgcggg caaactcgcc accgatgaag
    16861 aggagctccg cctcccgctc acgctgcacg ctcttcgccc acacctgccc gaactgggca
    16921 agccccaggc ccagggccgc caccgcgaac agggtgagca gatagaccac gccagactgg
    16981 ggccggcgcc gggcgcgtcc tgccctcata gatcctcgta ggcctgaccg ctgcgggtct
    17041 tgccctccgc gccgctgtgc aggtcgctca tgccgccgcc gagggcttcc ggcgcagtca
    17101 ccggcgacca ggtgtcgcga cgttcggtga tgggatccac cggcaccgcg cgcaggtagc
    17161 gccggctcac cagctcgtcc agatccttgg gatagcgccc ggtgtcgcca tagaacttgt
    17221 cgatggcgtc ccgcgtgacc gccagggtct cggtgagcgc cgcctcgcgg gcccgctcca
    17281 gatgcccgaa atagcgcggc gcggcgatgg acagcagcac cgccacgatg gccatcacca
    17341 ccagcagttc gaccagggtg aagccgcgcc gacgtgggga aagtggttcc atcaccactc
    17401 ccggtaggcg atgccgttga ggcccttgcc ggtggcccgg gagtacacgt cgaagacgtc
    17461 ggcaccctcg gccggctcgt ccggcgggct ggcgtagctg cgcagacccc aggtctcgtc
    17521 cgccgccagg ctcaggtccg ggttcagcgg atcacgggga atgcggcgca ggatgtagag
    17581 tctggcgccc ttcggcgact tggcgtcggg cacacccttc accagatcct ccagcgcggg
    17641 cggatagccg ctggcatcca cgaccttggc gatgcgccct tcgtcgctgt agcgcttata
    17701 ggtgtcgatg gcctcgcgga tctggcgcag ggccgtgcgc agttcggcct cccgcgcccg
    17761 ctgggccgag agctgcacca ccggcgcggc gatggcggcg agcaccgcca ccaccgccac
    17821 ggtgaccgcc atctcgatga gggtaaagcc gcgccggcgc cgcatgccgt gcctcaaggc
    17881 tggatctgca ccgacacggg cggtggtggc ggcaccatct gggcctggcc gccaaccctc
    17941 aactgggcct gccccagggc gaacaccgcc gtgccgctgg cgccgggccg cacccggaat
    18001 cgcgcgaaca cgacgcccgc ggtgcccggc ggcagggtca gcttgcgcag atccccgtcc
    18061 gccggcccat cggccgactc cacgaggcgg ctgtcgaaat tgaggtccac gctgcccccc
    18121 tcgttcccgc cggcctgcac gccgacgctc accgtgaatt cctcgcccgc cttcacttcc
    18181 ggcggagcct gcacgctggt ggccgccgcc agcgccccgg tggcgggcga cgcagccggc
    18241 atcgcacccg gtgccgcggc cgccacaccc agcgcgcccg gcgccgtggg cccgatgctc
    18301 agcgccgaca cccccaccgc cgcttcggtg ccggcggcct gggtggcgct ggcaagcccc
    18361 ggcttggtca cgttgcgcac cacccgcggg gtgatgagca ggatgatctc ggtcttgttc
    18421 tccacatccc gctggctgct gaacaggcgt cccagggccg gcacctcgcc cagccccggc
    18481 aggcgggcgg cggagctgcg ctcctcgtcg ctgatgagcc cggccagcac ctgggtctcc
    18541 ccgtccttga ggcgcaggga cgtggacgcg ctgcgggtac ccacctgata ggcgatggaa
    18601 ttggacggcc cggtgacctc cttgaccacg ctgctcacct ccagattgac cttgatggcc
    18661 acgtcgtcgt ccatatgcac cgacggctcc acctccagct tgaggcccac gtccaggtaa
    18721 ttcaccgagg ccgacacgcc cacgttggcg gtggccgtgg tggtgaagat cggcagcttc
    18781 tcgccgatat ggatcttggc cttgtcccgg ttcttcaccc ggatgcgcgg attggccagg
    18841 atattggcgt tgccgtcctg gtccgtgagg ttgaggagca ggccggggtt ggccacgtag
    18901 ggcaccacgc cgctgcgccc gccgcgcagg tcgatgttgc cctgcagaag ctggccgccc
    18961 aggctctggg taatcgtagt gccggtggcg gtggtcaccg aattggtcac ggtcggctgc
    19021 agcacgccgt agccgatctg gttggggaag cgcatgccca gttccagcag cttgttgcgg
    19081 ctgatctcca tcacctcgac ttccagcatc acctccggct ccgccaggtc gagggtggcg
    19141 atcaggcgtt cggccaggcg gatcgcctcc ggcgtgtcct tcatcaccag caggttgagc
    19201 ttttcctcga tgaacacgtc cttggtcttc accacctgct tgatcaggtt catggcctgc
    19261 ttggtatcgg cgttggccag gtagaagctg cgcgtggcga gctcctggta gtcccgctgc
    19321 ttggccggcg tgttgggata gatcagcacc gaattgtcgt tgagcagctt gcgttcgagc
    19381 tgctgggtga tcatcagcaa cttgagcacg tcgtcgatgc tgttgttgcg cacgaagatg
    19441 gtcaccttgg cgtcggtgcg cacatcccgg tccatcacga agttgaggcc ggacacccgc
    19501 gacagcacct cgaacacgcc ccgcagcggc gcgtcacgga attccaggtt caccggcttg
    19561 gccagcggcc ccttgagggc ggccggcgcc ggatcgcggg cgacggcccg ttcgtccagg
    19621 cgacgcatca ccgcccgcgc cgcggcatgg gtcggctcgc cggccagcac cgtgcgcgcc
    19681 gccgcctcgg ccccggcaaa gtcgccggcg gccaggcgcc ggtcggcctc cgccgccagc
    19741 tggttgcgct gacgctgggc ggcgatggcg tccacaccgg ccaccacgcg ggggttgttg
    19801 gggtcgaact ggcgcgccag ggccagggcg ttctccgctt cttcgaagcg ccccgcgccg
    19861 cgggcctggt cggccatggc cagctggcgg gtcgtcaggg cctcccgctg gcgcaggtaa
    19921 taggcgcgca gctccaggtt gcgcggatcg gcctcgacgc ggctgcgcag gtcgatcagg
    19981 gccacgtcgg ggctctgggt ggcaaaggag cggcggctct cctccagcgc cttgttgccg
    20041 gcgcaggccg cgagcagcac ggtcagcgcc aggggcgcca ggcggcgcag gagtcgggga
    20101 aggcgtctcg aagtcatggc atggcccccg tcacgaggct ctggggttcg ttgagcggca
    20161 gatagatcag gtcgatgcgg gcgcggctca ccgccgtcac ccggtagcgt ccgtcgaggg
    20221 tgtcaccgac ccgcaccacg cggatctgct caccctgctc caggaaggcg gcgtggcggc
    20281 cgccctcttc cagcatgccg gtgtagcgga agggcaacgg cggcgccgtc ggcgtgggcg
    20341 gtggtggggg aggcggtggc ggtggcgcga agctgcgcac ggggaacaga tcgggcaccg
    20401 gcgcaccggc atccccgtcc gccagtgccg gccgggacgc ggcgagggcg gcgagatcca
    20461 cgcggggcgc gctgcgcacc gaagccgcct tgcgctgcac ggctcgacgc ggcgcctcgg
    20521 tggccggctc gtcctccagc ccggcggccc accaggcggc cagcagggtc aacgccagtc
    20581 cggcgagcag cccatgcacg cgcttcatgg ctggcgcctc aggaacacca caaaacgcac
    20641 ctgcgcctcc acctccggcg tcgccatgga cgcacgccgc agggacaggc cgtccagcgc
    20701 cacttcgggc atctccgcca gcacgtcggc cagccaggtg cgcagcaccg ggtaggggcc
    20761 gcgcgcggga agctccagag tgacccgctc cagcggcgtg ccgccctccg gcgcagaacg
    20821 gtaatcggcc cggtccagct tgaggccccg cgccagcgcg tagccatgca ggcgcaccag
    20881 caggtcaggg agcgcatcgg tcgcggggaa ggccccgtag aagcgctcca gccgcgtccg
    20941 ctcaggcacg gcgacgccct ccggatgggt gcgcaggcgc tggcgcaggc tgcgggtttc
    21001 ctgctccagg gcgtcagcct gggcttccag cgcccggttc ccgctcaggt cgaaacacac
    21061 ggcaaacacc gtcagagcca cgcccaccac gccggcccag cccgccgcgc ggaaggtccg
    21121 caccgcggat tcgtgcaggg tcgcccaccg ggcgccggtc agacggaggc tcatggcgcg
    21181 acctcccagc gggcggtcac ggtgaagccg acgacctgaa gcggtccgct ggcatggaac
    21241 tcatagctgc ccagacgcac ctcgcgcaac acgccacccg cctcgagacg cttcacgtag
    21301 gcgaagacct cgggcaggct gcgcccctcg ccggtcaggc gcaggccacc gcgtgcgccc
    21361 tcctggtcga gcgccagcag ggcgatgccg ggatcgtcgc tggctgtttc cagcaccccc
    21421 agcacggcgc gccagtcccg ctgcagcagc tgtgccacgg cccgcgcggg gcgcgcctcc
    21481 tgctcggtca ccggcgcgcg atcgacgccg ctggcgcgca ggtcgagccc gtgcttggtc
    21541 cgcgtcagag catgggccgc ttcggccacg cgggtctcca gcacatcgta ttcgtggagc
    21601 acgccgaccg ccgcgatcac gccgaaggcc agcaccgccc agcccagcca gccgggccgg
    21661 cgcaggccgc cgacgaaatc cagccccagc ggcggcggcg cgcgatgcgg gctcatgacg
    21721 caccgccttc cacgccggcc agggtccagc cagcgggcag cgccgtcgcg gtcgcggccg
    21781 cacccgcaag atagagggtg ccctgggcca tcgcctgacc tgccacagcc agccctggca
    21841 gcagcccggc cagacagccc gccgccgcgg ccgcgtcggc cacagcgacc gcctggctgc
    21901 gcaccgcggc ccacacgccg tcgcgccaca gggcgacggt gacgcggccc gcctcggcgc
    21961 gggcaaaggc gccatcgccg atgaaacgcc ggccgtgacg gttgaactcg gcggcaaagg
    22021 cgccttccag gcggacaagg cgcaggtggc gcgtccgcgc gaaatcctgc accgcctcga
    22081 gcagcgcccg cggcagcgcg caggccaggc tgggcaacac cacggcattc cggtcggcga
    22141 ggacgcacca gtcctcgcca atcccgaaga cagtctggaa acgatgccgc gtgaaggccg
    22201 cccgctccgc cgcgcgcagg ccggccggcc agtcgatgcg caggt
//
LOCUS       DAOQMM010000056        21944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_809_length_21944_cov_45.643885, whole genome shotgun sequence.
ACCESSION   DAOQMM010000056 DAOQMM010000000
VERSION     DAOQMM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21944
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_21944_cov_45.643885"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2675)
                     /gene="glnE"
                     /locus_tag="PLH85_10325"
     CDS             complement(<1..2675)
                     /gene="glnE"
                     /locus_tag="PLH85_10325"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNB65004.1"
                     /translation="MSEQPDTLHPLVAAATAHSRFLRRQLDSRPWLAARLAASVDAPL
                     DATALRDFLRAEHVDDSTLKPALRRLRAWIIGHVMVRDLAGLASLTEVTETMTLLADI
                     AVETTVDLHRAALVARHGQPVSADGREQEFIVLGMGKLGGRELNVSSDIDLIFVYPED
                     GSTAGPKIIDNYEFFTKLGRAVIQALNDLTGDGQVFRVDMRLRPNGDSGPLVASFDML
                     ENYFITQGREWERYAWIKARVMTGIRGAELAAIVRPFIFRKYLDFGAINAMRELHAQI
                     RQEVARKDMANNVKLGPGGIREIEFIAQVFQLIRGGRDKALQIKPTLAVLGLLVERGI
                     LSRETGRELAAAYDFLRRVEHRLQYLDDAQTHTLPGNPDDQARLARSMGFTHYEALLE
                     ELDDHRAHVGRHFNAVFADPTGKGHDLDTLWSSAGDEALTTAEFTRLGYPDPAAAARR
                     LATLRAGIRYQQMPPSIRSRFDPLIPRVVEAAAPTPDANATFERLLDLLDAISKRGAY
                     LALLQQYPQTLEKVAQIVSASAWAAQFLNRHPILLDEAIDPRILEEELDLGEFRRELA
                     GQLDEIEPDMERQMDLMREQHHAQVFRLLTKDIAGHLTVERHADFLSALADIMVDLAV
                     TMCWRKIRNRHRDDPRFAVLAYGKLGGKELGYASDLDIVFVYDDDAPESAELYTRLSQ
                     RTNTWLSAQTPAGSLFETDLRLRPNGDSGLMVVSLDAFRQYQLENAWVWEHQALTRAR
                     FCAGDRAIGRKFEAIRVEVLRQPRDLAKLRDEVIAMRQRMVDAHPVRAGVFDLKHSRG
                     GLIDVEFIVQYLVLGYAHTHPGLTGNLGNIALLPMAAELGLIPPDDAAAVADTYREYR
                     RLQHALRLNNQAPRVDPALVAEGAAAVRDLWAQVLG"
     gene            2757..6659
                     /locus_tag="PLH85_10330"
     CDS             2757..6659
                     /locus_tag="PLH85_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNB65005.1"
                     /translation="MTRLRRLLTPFSGERLLSLLHLPRVWACRLLWALIAVYFVAGAG
                     LLALRWAVLPNVPAYREDVAALLSRSLGRPVAIGALSAQWHGFRPALRLDNLTLLDER
                     GEPALTLAQVNTELGWTSLPLLKLRLHRLEVVAPHLELRRDADGRLFVAGLQVNAEGE
                     SGDFLPWLLDQREVVVRDARVDWEDARRGAPALELRQVNLRLENRGSQHRFGLQAVPP
                     SALAATLDVRGDLRGRDPQQPASWRGEVFIGLDYADLGAWKTWVDYPLDLAGAGGVRA
                     WLEFGEARVKGITADFALRDARARLAPELEAIDAMHAEGRLRYADDAGRVEASGKRLA
                     LETRDGLKVAPTDFFLRWQPGRAGTPARGEFVANQLDLGVLAELAERLPVEPGFRRRL
                     ADLAPSGSVAPLNLKWTGEAGALATYSVDAKFAGLGIRPGGAWPGFAGLSGRIEGNDR
                     GGRFSLSGRQAALDLPQVFPESRLALEEMAADGTWSHPDGMLEVNLGSVSFANRDTRG
                     TATGRYRARPDTPGEIDLQARLAEAESTAVWRYMPGVVNQRARDWLQHSLTGGRARDA
                     RLVLRGNLAKFPFRDPKDGVFRVTAKVSDGSLDYAPGWPKIEDVTGELVFEAAGMSIK
                     AQHGRIFGVELKDVSVVLPDFETHEVLSIDGKASGPTQDFLRFIAESPVGARINHFTD
                     GFRTEGVGTLDLHLALPLHRMETSRVKGEFQFARNKVQPDPVLPVLTDAAGRIGFTEN
                     QLVVRSGTALLYGEPASVSGGTRPDGTVLLAIQGNASIAALRRELDEPLLDHLSGATA
                     WKGTIAVPRQGGLEVHVDSGLQGIASSLPEPFNKSPAAAMPLRADYLAGGSTAGDSFR
                     LAIGPSFQMQTARRREGGRWSPLRGGMALDEPLRMADKGVLLAATADRLDVDAWRRAL
                     SGGGAGNGGSGAGGGLPISGVALKAGELRMFGQRLNDVNLRAVMEEGGWQARLASREA
                     TGELTWRDQGRGRLQARFRQLAISGGTSGSGSEAGRSADRLSELPGLDVTADSFVLRG
                     RALGKLDLKAANRGDTWRLEHLSLASPDGTLSGDGSWRPGAKEETRLNFKLDVGSIEK
                     MLARLGYPEAVRQGKAQLDGEIGWRGPPTALHLASLNGHMNVRGEGGQFSQLEPGVGR
                     LLGVLSLQALPRRITLDFRDVFSQGFAFDRISGSIQMNDGVLRTEDLELVGPAARVFM
                     SGDADAVRETQSLRVKVQPTLSESVAVGSALATTGVIHPAVGLAAYLVQKALRDPVEK
                     LFSFEYAVTGSWSDPKVEKIAAVRLPAAPANPAERIGQ"
     gene            6656..7462
                     /locus_tag="PLH85_10335"
     CDS             6656..7462
                     /locus_tag="PLH85_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNB65006.1"
                     /translation="MSSVHRIAAVQMVSGPDVAANLAAAERLIGEAAAAGAGLVVLPE
                     YFPLITADETAKLRLCEPDGEGPLQAFLQDAARRHGVWLIGGTIPLAGSDPGRVKNAI
                     LVFDDTGQRVARYDKIHLFGFSKGAERYDEAATIEPGREVVTFDSPCGKVGLSVCYDL
                     RFPELFRAMGEVDLIVLPAAFTYTTGRAHWEILLRARAIENQCYVLASAQGGVHPSGR
                     RTYGDSLIIDPWGEVLARRAEGPGVVVADLDPARIAEVRTSLPALQHRIL"
     gene            7477..8934
                     /gene="tldD"
                     /locus_tag="PLH85_10340"
     CDS             7477..8934
                     /gene="tldD"
                     /locus_tag="PLH85_10340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNB65007.1"
                     /translation="MTKNPPPSPLKIASKLLLKPYDLDFEDLDKVFGKLFRHKLDYAD
                     LYFQYHRSEAWSLEEGIVKSGSFNIEQGVGVRAVTGEKTAFAYSDDISLKALRAAAEA
                     TRAIAASGRRKTHKVEAVKKPPLALYRGDNPHGSLDDVAKVRLLERVEAMARAEDPRV
                     REVMASLVGSWEVVLVARSDGHLAADVRPLVRVSVSVIMEENGRREQGSGGGGGRYDY
                     AYFSDEVLAGYARAAVHQARVNLGAGPAPAGSMTVVLGNGWPGILLHEAIGHGLEGDF
                     NRKESSAFSGRIGQQVAARGVTVVDDGTLPDRRGSLTIDDEGNPTQRTVLIEDGILTG
                     YMQDMLNARLMGVAPTGNGRRESFAHLPLPRMTNTIMLAGQHEPAEIIASVKNGLYAV
                     NFGGGQVDITSGKFVFSTSECYLIEDGKVTRPVKGATLIGNGPDCLTRVSMIGNDLAL
                     DPGVGTCGKDGQSVPVGVGQPTLRIDGLTVGGTGG"
     gene            8936..9724
                     /locus_tag="PLH85_10345"
     CDS             8936..9724
                     /locus_tag="PLH85_10345"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNB65008.1"
                     /translation="MERREFLMAAAAMAAAAPLGAAPAPAGIALPGAINRAGRQRMLS
                     QRSAKAWLMSVLGVLPDRARSILDQTVTRFDQQLADLKTQLPSDEVRTLLAQLESEWS
                     RYRPLLGDIRSDPKAVWDGNEAVLGVAHKLTVAYEKAFGSPSGHLVALAGRQRMLSQR
                     MAKAYFFRQLNVNAGPAAEMLDQATKEFAKAQAELRGAATNSPQINTELGLVDQQWFF
                     FQNALVLKEGVDPKRAAADVATTSERILEQMDLVVGLYEKLAAR"
     gene            9738..10265
                     /locus_tag="PLH85_10350"
     CDS             9738..10265
                     /locus_tag="PLH85_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65009.1"
                     /translation="MNRWRVVAMAFVLVTGCATQPSPPPPLLRLDPADAARIDAARGA
                     LRPLALDEIVALSRAGTPSADLVARIRATGTRHAIPAPEAARLQAQGVAPEVVDVLNE
                     AQARWAQDQAAAERVRRDTAAAAAEDRARAEAERDRRRAAPYYYDPLWPYGYPYRHGY
                     PGGHFGWGGGVHLWR"
     gene            complement(10292..11362)
                     /locus_tag="PLH85_10355"
     CDS             complement(10292..11362)
                     /locus_tag="PLH85_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNB65010.1"
                     /translation="MKRPIPLQGRGSRLNPDTRFADWQRAAFDDGWDAAGDAPVPPPT
                     VLMVDTARSVICRNTSPDVPFSQSINPYKGCEHGCSYCFARPGHAYLGLSPGLDFETH
                     IAWKPDAAAVLRRELAAPGYRCAPIALGINTDGWQPLERRLGLSRQLLHVLAETRHPV
                     SIVTKSALIERDADLLADLARDDLVQVMFSITTLDPDLARRMEPRAASPARRLAAMDC
                     LRRAGVPVGVLCAPLIPALNDHELEGVLAAAKGAGAETAGYVLLRLPRELQALFTDWL
                     QAHFPDRAAHVLSLLRQLRGGKLNDPRFGHRMRGQGVLADLYSQRLARARQRLGLDRP
                     RRTLNTGAFRPPAPDGAQLRLF"
     gene            11486..13036
                     /locus_tag="PLH85_10360"
     CDS             11486..13036
                     /locus_tag="PLH85_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNB65011.1"
                     /translation="MSATMPFYQRDEAPAVRRYGLAVLVCGLAAGLALPLRDVLDLAN
                     TEMLFLLAVVLVAARLGRGPAVLAAVGSVALFDFFFVPPLFSFAVNHSQYLASFAVML
                     AVSLIVGQLTAGLRLQAGEAGQRERTTRALYGLAKSLAGAREPVEVEAALRAFLHAHV
                     DARMWLVLPDAADVLAVVKAEDGPFGELDGHTARMAYDGGCAIESGATEVPGLRRHYL
                     PLAGASRARGVLVVSLPTGGPVGDLPLVEAVASLAATAVERLHFVAAAQAAQLEAVTE
                     RLRSSILSALSHDVRTPLTALYGLADTLLLTRPPLSGEALETAQAIRDQALRVNGMVV
                     NLLDMARLQAGKVVLRSEWQSVEEVVGASLKSLGGALAGHPVRTAGLGDLPLVQCDAV
                     LMERVFCNLFENAVKYSPQGSPLTVTARAGPDTLEIRVGNGGPGFPPDRLEQVFELFE
                     RGTPEAAVPGVGLGLAICRSIVEAHGGTIRAFNPPGGGGCIALRLPLGTPPPVEHEPV
                     AEGSGEML"
     gene            13033..13716
                     /gene="kdpE"
                     /locus_tag="PLH85_10365"
     CDS             13033..13716
                     /gene="kdpE"
                     /locus_tag="PLH85_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HNB65012.1"
                     /translation="MSAAQILIVEDEHQIRRFVRTALEADGCVVREADTCGQGLVAAG
                     VHAPDLVILDLGLPDGDGTRFVQDFRTWSAAPVLVLSARSTEHDKIGVLDAGADDYLT
                     KPFSVGELKARVRALLRRGRAHEQVSAVQVFGDVEVDLSRRRVIRQGEPVRLTPLEYR
                     LLTVLLANADKVMTHRQLLREVWGPSYSDSSHYLRIYVGHLRHKLEADPAQPRHFLTE
                     TGVGYRFQV"
     gene            13764..15638
                     /locus_tag="PLH85_10370"
     CDS             13764..15638
                     /locus_tag="PLH85_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNB65013.1"
                     /translation="MSDPSSHSRAGLLALSLAALGVVYGDIGTSPLYAFKEAFSGPHG
                     IDPSEGNVLAALSAFFWAVALIVSVKYVGIVLRFDNEGEGGVLALTAFAHRVSSNPRW
                     SVLAVAGGIFAAALFYGDAIITPAISVLSAVEGISVATPHLEHAVVPITVGILVALFL
                     IQKHGTGHVGRFFGPVTVVWFLTLAVLGVASIMETPRVLQAIDPRLAVEFAFEKPKVA
                     FVLLSAVFLALTGGEALYADMGHFGARPVRLAWYGLVWPSLMLNYFGQGALVLREPVA
                     IDNPFYMLAPDWFLLPLVGLATLATVIASQATITGAYSMTLQATRLGYLPRLRILHTS
                     DTERGQIYVPSVNWLMLVAVLVLVFQFRSSGALAAAYGIAVSGTMIITTLLTAFIVWM
                     SPTRSRVGLLAVLLLFGLLELMFFGSNLTKIAEGGWLPMVLGGTIFLMLTTWKQGSLL
                     LARKRCQIDVPMAGFIKGPLPDVPRVAGTAVYLLSDSDLVPSALFHNLKHYKVLHERI
                     VFLHVANQDVPRIDDEERVEVTQLAPSIFSVEAKYGFREEPDVPAALALAARYDLELE
                     PMTTTFFVARSNVVDGPGELPRWRCALFAWMVRQSEGAGSSFHLPANQVVELGTQVML
                     "
     gene            15746..16828
                     /gene="aroG"
                     /locus_tag="PLH85_10375"
     CDS             15746..16828
                     /gene="aroG"
                     /locus_tag="PLH85_10375"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HNB65014.1"
                     /translation="MHASAKVHIDDVRIKEIKELAPPAHVLREFPATPMAADTTFAAR
                     SAIHRILFGADDRLLVVIGPCSIHDYEAAMDYARRLKAEADRLKNDLLVVMRVYFEKP
                     RTTVGWKGLINDPHLDGSFAINEGLRLARRLLWEVNELGLPAGTEFLDMITPQYIADF
                     IAWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNIRIAVDAIKAAQSPHHFLSVTK
                     AGHSAIVSTSGNEDCHLILRGGKGPNYDAASVDAACTELAASGVQGRLMVDFSHANSR
                     KQHKLQVEVARDVAGQLAEGEDRIIGVMVESHLKEGRQDLKPGVPLEYGQSITDACIG
                     WEDSVTVLEMLAEGVRQRRIRRATSE"
     gene            complement(16913..18691)
                     /locus_tag="PLH85_10380"
     CDS             complement(16913..18691)
                     /locus_tag="PLH85_10380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNB65015.1"
                     /translation="MVAAGLLACLIALVALWPTVDAPRPLIVWWALVTAGQLGLASIA
                     HRVRTGSGPSALDEDLGHYLLSFGAVVAGAAWGALPLRGPYTSGTPAQLTLFLLLGGI
                     TLGGAAILSASRSAFAVFVLATLLPLFILTVDLPPPNMPHASTGLILFGAFAFAVHDL
                     IHAAHRPRSSPPAAAAVGGFEEKILDGAGEAIVLCTGSRIIHHNRRFIELVGERSTHA
                     RAEGRLAHWLADSSERRRARTALSALCQGRVYRDVVRLRRRDGSIFTAEVSGRALDPH
                     ARPLRVVWVATDITERVAGEVRRTMLGTQLQELVSQSGDGYWQTDALHRLVHVHACHA
                     PLDGILDARIGRPWWTQDPRPGVQSDARQTEIRQIFEDRGAFRDLMVEVRDGYHPPRW
                     LTLSGTPRQDEHGCFLGYHGIAVDVTERVRGTERIRHLAYHDALTGLPNRRLLVDRLG
                     QAIERAQRYRERLGVILIDLDDFKRINDLSGHSVGDRVLLDTADRLRACIRPCDTVAR
                     LGSDEFVVLLSELDQPSDADQMAGTILHALGQPVAGRLGSAPLGTSIGIALYPEDAQT
                     ADSLLEVADRRMYRAKRRGGQTIEHV"
     gene            19037..19954
                     /gene="gluQRS"
                     /locus_tag="PLH85_10385"
     CDS             19037..19954
                     /gene="gluQRS"
                     /locus_tag="PLH85_10385"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNB65016.1"
                     /translation="MSAPGGAARKGRFAPSPTGPLHFGSVVAAVGSYLDARSSGAAWL
                     LRIEDVDVPRCVPGAAEGILRTLERLGLEWDGPVLWQSSRTEVYRAALERLMAAGRVF
                     GCICTRRALADACLARDGTRRYSGTCRDGLPPGRTARAFRLRVEPGCVRFVDRIQGPI
                     EEDVAADVGDFVVLRADGYFAYQLAVVVDDAEAGITDVVRGADLLDSTPRQLLLQRAL
                     GLPQPAYAHVPVVTNGAGEKLSKQTLAQAVDGLAPGGVVADALRFLGHPPPAALGGAA
                     PAELLAWAQGIWSLARVPCVRGAPMPGYG"
     gene            complement(19976..20404)
                     /locus_tag="PLH85_10390"
     CDS             complement(19976..20404)
                     /locus_tag="PLH85_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65017.1"
                     /translation="MDAPAPIRLRGGQRHHAHLPAGTLIIACAGRLSVTDAPRWLGDQ
                     VFQVHHRLAEGDVLRIEAGGWIAVSADGDASFVCAPPPGLTLRLHDGAAQLEAWFVQT
                     WRAQVESPLALLRARLRWLQTLQRRRLRVEVSALRARLER"
     gene            20576..>21944
                     /locus_tag="PLH85_10395"
     CDS             20576..>21944
                     /locus_tag="PLH85_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB65018.1"
                     /translation="MDRIDTAETLYLRVAGYYRRAIRSGALAAGDRLPSLRALTRLHG
                     VSLSTVVQACHCLEDEGLLEARPRSGYFVRAARGSTLQPVREPDVGRPPSLPDPAVFA
                     GIHARVSDFVAKCERFPTHTNLAQAFGPPEVYPLEALRNAAIRALRRHPEVLVNPVPP
                     LGDAGFRSVLARRAVAAGMSPAPDEILVTHGCTEALNLALRAVAAPGDVIAVESPAYF
                     GLLQVIESLGLRALEIPTSPSRGLSVEALELAIRTHPDLRAVVVVPNFQNPLGCVMPD
                     GEKARLVELCSGAGVALIEDDIYGALDDDDQSLKACKAWDRDGSVIHCASFHKTLAPG
                     MRVGWMLGGRWLDRIRMLKHAQSRPNDALAQLAIGEFMAGSAYDRHLVRLRRHLLAQR
                     THMAEAIATHFPAGTRLNPPGGGMLLWVELPAGCDSMAVFDAALRSGIRVAPGTMFSN
                     SGRFDH"
BASE COUNT         3167 a         7099 c         8153 g         3525 t
ORIGIN      
        1 cccagcacct gcgcccacag atcccgcacc gccgcggccc cttcggcgac cagcgccggg
       61 tctacccgcg gggcctggtt gttgaggcgc agggcgtgct gcaggcggcg gtattcccgg
      121 taggtgtcgg ccaccgcagc ggcgtcgtcg ggggggatca ggccgagttc ggcggccatg
      181 gggaggaggg cgatgttgcc caggttgccg gtgaggccgg ggtgggtgtg ggcgtaaccg
      241 aggaccaggt actggacgat gaactccacg tcgatgaggc cgccgcggct gtgtttgagg
      301 tcgaacacgc cggcgcgcac ggggtgagcg tccaccatgc gctggcgcat ggcgatgact
      361 tcgtcgcgga gtttggccag atcgcggggc tggcgcagga cttcgacgcg gatggcttcg
      421 aacttgcggc cgatggcccg gtcgccggcg cagaagcggg cgcgggtgag ggcctggtgt
      481 tcccacaccc aggcgttctc gagctggtac tggcggaagg cgtcgaggga cacgaccatc
      541 aggccggagt caccgttggg gcgcaggcgc aggtcggtct cgaagaggct gccggcgggg
      601 gtctgggcag acagccaggt gttggtgcgc tggctgaggc gggtgtagag ctcggcggac
      661 tcgggggcgt cgtcgtcgta gacgaagacg atgtccaggt cggaggcgta gccgagctcc
      721 ttgccgccca gcttgccgta ggcgaggacg gcgaagcggg ggtcgtcccg gtggcggttc
      781 ctgatcttgc gccagcacat ggtgacggcc aggtccacca tgatgtcggc cagggcggag
      841 aggaagtcgg cgtggcgttc gacggtgagg tggccggcga tgtccttggt gagcaggcgg
      901 aacacctggg cgtggtgctg ctcgcgcatc aggtccatct ggcgttccat gtcgggctcg
      961 atctcgtcga gctggccggc gagttcgcgg cggaattcac ccaggtcgag ctcttcttcg
     1021 aggatgcgcg ggtcgatggc ttcgtcgagg aggatggggt ggcggttgag gaactgggcg
     1081 gcccacgctg aggcgctgac gatctgggcg accttttcga gggtctgggg atattgctgg
     1141 agcagggcca ggtaggcgcc gcgcttgctg atggcgtcga ggaggtcgag gaggcgctcg
     1201 aaggtggcgt tggcgtcggg cgtgggggca gcggcttcga cgacgcgggg gatcaggggg
     1261 tcgaagcggc tgcggatgct ggggggcatc tgctggtagc ggatgccggc gcgcagggtg
     1321 gcgaggcggc gcgcggcggc ggcggggtcg ggatagccga ggcgggtgaa ttcggccgtg
     1381 gtgagggctt cgtcgccggc ggaggaccac agggtgtcca ggtcgtggcc cttgccggtg
     1441 gggtcggcga agacggcgtt gaagtggcgg cccacatggg cccggtggtc gtccagctcc
     1501 tccagcaggg cttcgtagtg ggtaaagccc atgctgcggg cgaggcgcgc ctggtcgtcc
     1561 gggttgccgg gcagggtgtg ggtctgggcg tcgtccagat attgcaggcg atgctccaca
     1621 cggcgcagga agtcgtaggc ggcggccagt tcgcggccgg tctcgcggga caggatgccg
     1681 cgctccacga gcaggcccag cacggccagg gtgggcttga tctgcagggc tttgtcgcgc
     1741 ccgccgcgga tgagctggaa gacctgggcg atgaattcga tctcgcggat gccgcccggg
     1801 ccgagcttga cgttgttggc catgtccttg cgggcgactt cctggcggat ctgggcgtgc
     1861 agctcgcgca tggcgttgat ggcgccgaag tccaggtact tgcggaaaat gaagggacgc
     1921 acgatggcgg ccagctcggc gccgcggatg ccggtcatga cgcgggcctt gatccaggcg
     1981 tagcgctccc attcgcggcc ctgggtgatg aagtagtttt ccagcatgtc gaagctggcc
     2041 accagggggc cggagtcgcc gttggggcgc aggcgcatgt ccacgcggaa gacctggccg
     2101 tcgccggtga gatcgttgag ggcctggatg acggcgcggc cgagtttggt gaagaactcg
     2161 tagttgtcga tgatcttggg gccggcggtg ctgccgtcct cgggatagac gaagatcagg
     2221 tcgatgtccg aggagacatt gagctcccgc ccgcccagct tgcccatgcc cagcacgatg
     2281 aactcctgct cgcggccgtc ggccgagacc ggctgcccgt ggcgcgccac cagggcggcg
     2341 cggtgcagat ccacggtggt ttcgacggca atgtcggcca gcagggtcat ggtctcggtg
     2401 acttccgtga ggctggccag gccggccaga tcgcgcacca tcacgtggcc gatgatccac
     2461 gcgcgcaggc gccgcagggc gggcttgagg gtgctgtcgt ccacatgctc ggcacgcagg
     2521 aagtccctca gggcggtggc gtccaggggc gcgtccacgc tggcggcgag gcgcgcggcg
     2581 agccacgggc ggctgtccag ctggcggcgc aggaagcggg agtgggcggt ggccgcggcc
     2641 accagggggt gcagggtgtc aggctgttcg gacatggtgg gtgggcggtt ggtagaatgg
     2701 agtcctgtca tgttcgcccc gtcttatgtc tgccggactg attttcgtca agcctgatga
     2761 cccgactccg gcgcctcctc acccctttct cgggtgagcg cctcctctcc ctgctgcatc
     2821 tgccccgcgt gtgggcgtgt cgcctgctgt gggccctgat cgcggtgtac ttcgtcgccg
     2881 gggccggtct gctcgcgctg cgctgggcgg tgctgcccaa cgtcccggct taccgcgagg
     2941 acgtggcggc gctgctgtcc cgttccctcg gccggccggt ggcgatcggc gccctgtccg
     3001 cccagtggca cggcttccgg ccggcgctgc gcctggacaa tctgaccctg ctggatgagc
     3061 ggggcgagcc ggccctgacg ctggcgcagg tgaacaccga gctggggtgg acctcattgc
     3121 ccctgctcaa attgcgtctg caccggctgg aggtggtggc gccccatctc gaattgcgtc
     3181 gggacgcgga cgggcgcctc ttcgtggcgg gcctgcaggt gaacgccgag ggtgaatcgg
     3241 gcgacttcct gccctggctg ctggatcagc gcgaggtggt ggtgcgcgac gcccgcgtgg
     3301 attgggagga tgcccgccgc ggcgcgccgg cgctggagct gcggcaggtg aatctgcgcc
     3361 tcgagaatcg cggcagccag caccgcttcg ggctgcaggc cgtgccgccg tcggccctcg
     3421 ccgcaaccct ggacgtgcgt ggcgatctgc gcggacgcga tccgcagcag ccggccagct
     3481 ggcgcggtga ggtcttcatc ggcctcgatt acgccgatct gggcgcctgg aagacctggg
     3541 tggactatcc gctggatctg gcgggggctg ggggcgtgcg ggcctggctg gagttcggcg
     3601 aggcgcgggt gaaggggatc actgccgatt ttgccctgcg cgacgcgcgg gcgcggctgg
     3661 cgccggagct ggaggccatc gacgcaatgc atgcggaggg ccgcttgcgc tacgccgacg
     3721 acgccgggcg ggtggaggcg agcggcaagc gcctggccct ggagacccgc gacgggctga
     3781 aggtggcgcc caccgacttc ttcctgcgct ggcagccggg gcgggcgggg acgccggccc
     3841 gcggcgagtt tgtcgccaac cagctcgatc tgggggtgct ggccgaactg gccgagcgcc
     3901 tgcctgtgga gccgggcttc cgcaggcgcc tggcggatct ggcgcccagc ggcagcgtgg
     3961 cgcccctcaa cctcaaatgg accggcgagg ccggggcgct ggccacctat tcggtggatg
     4021 cgaagttcgc cggcctgggc atccggccgg gcggcgcgtg gcccggcttc gcggggctgt
     4081 cgggacgcat cgagggcaac gaccggggcg ggcgcttcag cctgagcggc cggcaggcgg
     4141 cgctggatct gccccaggtc tttccggaat cgcgtctggc cctggaggag atggccgcgg
     4201 acggcacctg gagccacccg gacgggatgc tggaggtgaa tctgggcagc gtgagcttcg
     4261 ccaaccggga cacccgcggc acggcgacgg ggcgctaccg ggcgcggccg gacacgccgg
     4321 gtgagatcga cctgcaggca cgcctggcgg aggccgagag tacggcggtg tggcgctaca
     4381 tgccgggcgt ggtgaaccag cgggcgcggg actggctgca gcacagcctg accggtggcc
     4441 gcgccaggga tgcgcgcctg gtgctcaggg gcaatctggc gaagttcccg ttccgcgatc
     4501 cgaaggacgg tgtcttccgc gtcaccgcca aggtcagcga cggcagcctc gactacgctc
     4561 ctggctggcc gaagatcgag gacgtcaccg gcgagctggt cttcgaggcc gcggggatga
     4621 gcatcaaggc ccagcacggg cgcatcttcg gcgtggagct gaaggacgtg agtgtcgtgc
     4681 tgcccgattt cgagacccac gaggtgctct ccatcgacgg caaggccagc gggccgaccc
     4741 aggatttcct gcgtttcatc gccgaaagcc cggtgggcgc acgcatcaac cacttcaccg
     4801 acggtttccg caccgagggc gtcggcacgc tggacctgca tctcgccctg cccctgcacc
     4861 ggatggagac gtcgcgggtg aagggcgagt tccagttcgc ccgtaacaag gtccagcccg
     4921 atccggtgct gccggtgctc acggacgccg ccgggcgcat cggcttcacc gagaaccagc
     4981 ttgtcgtgcg cagcggcacg gccctgctgt atggcgagcc tgccagcgtc agcggcggca
     5041 cccgcccgga cggcacggtg ctgctggcga tccagggcaa cgccagcatc gcggccctgc
     5101 gccgggagct ggacgagccg ctcctcgacc acctgtccgg tgccacggcc tggaagggca
     5161 cgatcgcggt gccgcgccag ggcgggctcg aggtgcatgt ggacagcggc ctgcagggca
     5221 tcgcgtcgag cctgccggaa cccttcaaca aatcgcccgc cgcggcgatg cccctgcgcg
     5281 ccgattacct ggcgggcggc agcacggcgg gcgacagctt ccggctggcc atcggtccga
     5341 gctttcagat gcagacggcc cggcgccggg agggtggacg gtggtcgccc ctgcgcggcg
     5401 ggatggctct cgacgaaccc ctgcgcatgg ccgacaaggg cgtcctgctg gcggccacgg
     5461 ccgaccggct cgatgtggac gcgtggcgtc gcgccctctc cggtggcggc gccggcaatg
     5521 gcggcagcgg ggccggcggc gggctgccga tctccggcgt ggccctgaag gccggcgagc
     5581 tgcgcatgtt cggccagcgc ctgaacgacg tgaatctgcg ggcggtgatg gaggagggcg
     5641 gctggcaagc caggctggcg agccgcgagg cgacgggtga gttgacgtgg cgcgaccagg
     5701 ggcgcggccg cctccaggcg cgtttccgcc agctggccat cagcggcggt acctcgggca
     5761 gcggcagcga ggcgggccgc agcgctgacc gcctgtccga actgcccggc ctggacgtga
     5821 cggccgacag cttcgtgctg cgcgggcggg ccctcggcaa gctggacctg aaggccgcga
     5881 accggggcga tacctggcgc ctcgaacacc tgagtctggc cagccccgac ggcaccctga
     5941 gcggcgatgg ctcctggcgt cccggcgcaa aggaggagac ccgcctcaac ttcaagctgg
     6001 acgtgggcag catcgagaag atgctggccc gcctgggcta tcccgaggcg gtgcgccagg
     6061 gcaaggcgca gctggacggc gagatcggct ggcgcgggcc gcccacggcg ctgcatctgg
     6121 cgagcctcaa cggccacatg aacgtgcgcg gcgaaggcgg ccagttcagc cagctcgaac
     6181 cgggtgtcgg gcgcctgctc ggggtgctca gcctgcaggc cctgccccgg cgcatcaccc
     6241 tggatttccg cgacgtcttc tcccaaggat tcgccttcga ccgcatttcc ggtagcatcc
     6301 agatgaacga cggggtgctg cgcaccgagg atctggagct tgtcggtccg gcggcgcgcg
     6361 tattcatgag cggcgacgcg gacgcggtgc gcgaaaccca gagcctgcgg gtcaaggtgc
     6421 agccgaccct ctccgaatcg gtggcggtgg gctcggccct ggccaccacc ggggtgatcc
     6481 atccggccgt ggggctggcc gcctatctgg tgcagaaggc cctgcgcgac cccgtggaaa
     6541 aactgttcag ctttgaatac gcggtgaccg gcagctggtc cgaccccaag gtggagaaga
     6601 tcgccgccgt ccgtctgcct gccgcgccgg ccaaccccgc cgaaaggatc ggtcaatgag
     6661 ttctgtccat cgcatcgccg ccgtccagat ggtgtccggc cccgacgtgg ccgccaatct
     6721 ggcggctgcc gagcggctca tcggcgaagc cgccgcggct ggtgcggggc tggtggtgtt
     6781 gcccgaatac ttcccgctga tcaccgccga cgagaccgcc aagctgcgcc tgtgcgaacc
     6841 ggacggggag ggtccgctcc aggccttcct gcaggatgcg gcgcggcgtc acggcgtgtg
     6901 gctgatcgga ggcacgattc ccctcgccgg cagcgacccg ggccgggtga agaacgccat
     6961 cctggtcttt gacgacaccg gccagcgggt cgcccgctac gacaagatcc acctcttcgg
     7021 cttcagcaag ggcgccgagc gctatgacga ggcggccacc atcgagcccg gccgggaggt
     7081 ggtcaccttt gattcgccct gcggcaaggt gggcctgtcc gtgtgctacg acctgcgttt
     7141 tccggagctc ttccgggcga tgggcgaagt ggacctgatc gtcctgcccg ccgccttcac
     7201 ctacaccacg ggtcgcgccc actgggagat cctgctgcgc gcccgcgcca tcgaaaacca
     7261 gtgctatgtc ctggccagcg cccagggcgg cgtgcatccc agcggccgcc gcacctatgg
     7321 cgacagcctg atcatcgacc cctggggcga agtgctcgcc cgtcgcgccg aaggccccgg
     7381 cgtggtggtg gccgacctgg acccggcgcg catcgccgag gtccgtacca gcctgcccgc
     7441 cctccagcat cgcattcttt gagctgcacc ccgaccatga caaaaaatcc gccccccagc
     7501 cccctcaaga tcgcttccaa gctcctcctc aagccctatg acctggactt cgaggatctc
     7561 gacaaggtct tcggcaagct cttccgccac aagctcgatt acgccgatct gtacttccag
     7621 taccaccgct ccgaggcctg gagtctggag gagggcatcg tcaagtcggg cagcttcaac
     7681 atcgagcagg gcgtcggcgt gcgcgccgtc accggtgaga agaccgcctt cgcctactcc
     7741 gacgacatca gcctgaaggc cctcagggcc gccgccgagg ccacccgcgc catcgccgcc
     7801 agcggccggc gcaagaccca caaggtggag gcggtgaaga agccgcccct cgccctgtac
     7861 cggggcgaca atccccacgg ctccctcgac gacgtggcca aggtgcgtct gctggagcgg
     7921 gtcgaggcca tggcccgcgc cgaggatccc cgcgtgcgcg aggtgatggc gagcctggtg
     7981 ggctcctggg aagtggtgct ggtagcgcgc agcgacggcc atctggcggc tgacgtgcgg
     8041 cccctggtgc gggtgtcggt gtcggtcatc atggaggaga atggccgccg cgagcagggc
     8101 tccggcggtg gcggcgggcg ctacgactac gcctacttca gcgacgaggt gctggccggc
     8161 tacgcccgcg cagcggtgca tcaggcccgc gtcaacctgg gcgccggtcc ggccccggca
     8221 gggtccatga cggtcgtgct gggcaacggc tggcccggca tcctgctcca tgaagccatc
     8281 ggccacgggc tggaagggga cttcaaccgc aaggaaagct ccgccttctc cggccgcatc
     8341 ggtcagcagg tggcggccag gggcgttacg gtggtggacg acggcaccct cccggaccgg
     8401 cgcgggtccc tcaccatcga cgacgaaggc aaccccaccc agcgcaccgt gctcatcgag
     8461 gacggcatcc tcaccggcta catgcaggac atgctcaatg cccgcctgat gggcgtcgcc
     8521 cccaccggca acggccgccg cgagtccttt gcccacctgc ccctgccacg catgaccaac
     8581 accatcatgc tggcgggcca gcacgaaccg gcggagatca tcgcgtcggt caagaacggc
     8641 ctgtacgcgg tcaacttcgg tggtggtcag gtagacatca cctccggcaa gttcgtcttc
     8701 tccacgtccg agtgctacct catcgaggac ggcaaggtga cccggccggt caagggcgcc
     8761 acgctgatcg gcaacggacc ggactgcctg acccgggtgt ccatgatcgg caacgacctg
     8821 gccctcgatc cgggcgtcgg cacctgcggc aaggacggtc agagcgtgcc ggtgggtgtc
     8881 ggccagccga ccctgcgcat cgacggcctg accgtgggcg gcacgggagg ctagcatgga
     8941 gcggcgcgaa ttcctgatgg ccgcagcagc gatggccgca gcggccccgc tgggcgcggc
     9001 gccggcccct gcgggcattg ccctgccggg ggccatcaac cgcgcggggc ggcagcgtat
     9061 gctctcgcag cgatccgcca aggcctggct gatgagcgtg ctgggcgtgc tgccggaccg
     9121 ggcgcggagc atcctcgacc agacggtcac ccgttttgac cagcagctgg ccgacctgaa
     9181 gacccaactg cccagtgacg aggtgcgcac cctgctggcc cagctggaaa gcgagtggtc
     9241 ccgctaccgc cccctgctgg gtgacatccg cagcgacccg aaggcggtgt gggatggcaa
     9301 cgaggccgtg ctgggggtgg cgcacaagct caccgtggcc tacgagaagg ccttcggcag
     9361 cccgtccgga cacctggtcg cgctcgccgg gcgtcagcgc atgctgtccc agcgcatggc
     9421 caaggcctat ttttttcgtc agttgaatgt gaacgccggc cccgcggcag agatgctgga
     9481 tcaggccacc aaggaatttg ccaaggccca ggcggaactc cggggcgcag ccaccaattc
     9541 tccccagatc aataccgagc tgggcctggt ggatcagcag tggttcttct tccagaacgc
     9601 cctggtcctg aaggagggtg tcgatcccaa gcgggctgcg gccgatgtgg cgaccaccag
     9661 cgaacgaatc ctggagcaga tggacctggt ggtcggtctg tacgaaaagc tggcggcacg
     9721 ctgaaggagg cggtgcgatg aatcgatggc gcgtggtggc gatggcgttc gttctggtga
     9781 ccggctgtgc cacgcagccg tcccctccgc ccccgctgtt gcgcctggac cctgcggatg
     9841 cggcgcgtat cgatgcggcg cgtggggcgc ttcgcccgct cgcgctcgac gagatcgtgg
     9901 ccctctcccg tgcgggaacc ccgtcggcgg atctcgtggc gcgcatccgc gcgacgggaa
     9961 cccgccatgc catccctgcg cccgaggcgg cgcgtttgca ggcccagggt gtcgcgccgg
    10021 aggtggtgga tgtgctcaac gaggcccagg cccgctgggc ccaggaccag gccgccgccg
    10081 aacgcgtccg ccgcgacacc gcggcagcgg cggccgagga tcgtgcgcgg gccgaggcgg
    10141 agcgggaccg gcgtcgtgct gcgccgtact attacgaccc cctgtggccc tatggctacc
    10201 cgtatcgtca cggctatccg ggtgggcatt tcggctgggg cgggggcgtg catctgtggc
    10261 gttgaggcgc gaccgccgct gacggccggg ttcagaacag gcgcagctgg gcgccgtccg
    10321 gcgccggcgg acggaaggcg ccggtgttga gggttcgccg ggggcgatcc agtcccaggc
    10381 gctgccgggc gcgggcgagg cgctgactgt acaggtcggc cagcacaccc tggccgcgca
    10441 tgcggtggcc gaagcggggg tcgttgagtt tgccgccgcg gagctggcgc agcaggctga
    10501 gcacgtgggc cgcccgatcg ggaaagtggg cctgcagcca gtcggtgaac agcgcctgca
    10561 gttcgcgcgg caggcgcagc agcacgtagc cggcggtttc tgcgccggcc cctttggcgg
    10621 cagcgagcac cccttccagc tcgtgatcgt tcagcgccgg aatcagcggc gcgcacagca
    10681 cgcccaccgg cacgcctgcc cggcgcaggc agtccatggc ggcgagacgg cgcgctggcg
    10741 acgcggcgcg gggttccatg cgccgggcga gatccgggtc cagggtggtg atggaaaaca
    10801 tcacctgcac cagatcgtcc cgtgccagat cggcgagcag atccgcatcc cgttcgatga
    10861 gggccgactt ggtgacgatg gacaccggat ggcgggtctc cgccagcacg tggagaagct
    10921 gacgcgacag gcccaggcga cgctccaggg gctgccagcc gtcggtattg atgcccaggg
    10981 cgatgggggc gcagcggtag cccggcgccg ccagttcgcg ccgcaacacg gcggcggcat
    11041 cgggtttcca ggcgatgtgg gtctcgaaat cgaggcccgg cgagagcccg agataggcgt
    11101 ggccgggccg cgcgaagcag tagctgcaac cgtgctcgca gcccttgtag ggattgatgg
    11161 actggctgaa ggggacgtcg ggcgacgtat tgcggcagat gaccgagcgc gccgtgtcca
    11221 ccatcaggac ggtcggtggg ggcaccggcg cgtcgccggc ggcgtcccag ccgtcgtcga
    11281 aggccgcgcg ctgccagtcg gcaaaacggg tgtcaggatt gagccggctg ccgcggccct
    11341 gaagggggat cggccgtttc atgtggttta gtgtacaaaa atacactata cactcgccca
    11401 cggtcgccgc agatcgttta cgccattttt atggaggcgg tgcaacaatc ggccccgacg
    11461 cgttttgcga ggcctcctgc gccccatgtc cgccaccatg cccttttacc agcgcgatga
    11521 agcgcctgcc gtccgtcgtt acgggctggc agtgctggtc tgcggactgg cggcggggct
    11581 ggctttgccc ttgcgggacg tgctggacct ggccaacacc gagatgctct tcctgctcgc
    11641 cgtggtactt gtggcggcgc ggctggggcg gggaccggcg gtgctggcag ccgtgggcag
    11701 cgtggccctg ttcgatttct tcttcgtgcc gccgctgttc tcgtttgcgg tcaatcacag
    11761 ccagtatctg gccagcttcg cggtgatgct ggcggtatcg ctgatcgtcg gccagctcac
    11821 cgccgggttg cgtcttcagg cgggggaggc cggccagcgc gaacgcacca cgcgagccct
    11881 ctacgggctg gccaaatctc tcgccggggc tcgcgagccg gtcgaggtgg aggcggcgct
    11941 gcgggcattc ctgcatgccc acgtggacgc gcggatgtgg ttggtgctgc ccgacgccgc
    12001 ggatgtgctg gcggtggtca aggctgagga cggccctttc ggtgaactgg acggccacac
    12061 cgcgcggatg gcgtacgacg gtgggtgtgc catcgagtcg ggggcgaccg aggtgccggg
    12121 tctgcgccgt cattatctgc cgctggcggg ggcgtcgcgg gcgcgtggcg tgctggtggt
    12181 gtcgctgccg accggcggac cggtgggcga tctgcccctg gtggaggcgg tggcgtcgct
    12241 ggcggcgacg gctgtcgagc gcctgcattt cgttgccgcg gcccaggcgg cgcagctgga
    12301 ggcggtcacg gaacggctgc gcagctcgat cctgtcggcc ctgtcccacg acgtgcgcac
    12361 gccgctgacg gccctgtacg gattggcgga caccctgctg ctgacccgcc cgcccttgtc
    12421 cggcgaggcg ctggagacag cgcaggcgat ccgcgatcag gccctgaggg tgaatggcat
    12481 ggtggtcaat ctgctggaca tggcccgatt gcaggcgggc aaggtggtgc tgcgcagtga
    12541 atggcagtcg gtggaggagg tggtgggcgc gagcctcaaa tccctcggcg gcgccctggc
    12601 cggccatccg gtgcgcaccg ccgggctggg ggatctgccg ctggtgcagt gcgatgcggt
    12661 gctgatggag cgggtgttct gcaacctctt cgagaacgcc gtcaaatatt caccccaggg
    12721 gtcgccgctg acggtgacgg cccgcgccgg cccggatacg ctggagatcc gcgtgggcaa
    12781 cggcgggccg ggttttcccc cggaccggct cgagcaggtg ttcgaactgt tcgagcgcgg
    12841 cacgcccgag gccgcggtgc cgggagtcgg tctgggactg gccatctgcc gcagcatcgt
    12901 ggaagcccac ggcggcacga tccgtgcgtt caatccgccg gggggcgggg ggtgcatcgc
    12961 gctgcgcctg cccttgggca cgccgccgcc ggtagagcac gagcccgtcg cagaaggctc
    13021 gggagagatg ctttgagcgc agcccagatc ctcatcgtcg aagacgaaca ccagatccgc
    13081 cgtttcgtgc gcacggcgct ggaggcggac ggctgcgtcg tgcgggaggc ggacacctgc
    13141 gggcagggcc tggtggcggc gggagtccat gcgccggacc tggtgatcct cgatctgggt
    13201 ctgccggatg gcgacgggac gcgtttcgtg caggacttcc ggacctggtc ggccgcgccg
    13261 gtgctggtgc tgtcggcccg ctcgacggag cacgacaaga tcggcgtcct cgatgcgggc
    13321 gccgacgact acctgaccaa gcccttttcg gtgggcgaac tgaaggcgcg ggtgcgcgcc
    13381 ctgctgcgcc gcgggcgggc ccatgagcag gtctccgcgg tgcaggtctt cggcgacgtg
    13441 gaggtggatc tgtcgcgccg ccgggtgatc cgccagggag agccggtgcg tctgacgccc
    13501 ctcgaatacc gtctgctgac ggtgctgctg gccaatgccg acaaggtgat gacccaccgc
    13561 cagctgctgc gcgaggtgtg ggggccatcg tacagcgaca gcagccacta cctgcgcatc
    13621 tacgtggggc atctgcgcca caagctggag gcggacccgg cgcagccgcg ccatttcttg
    13681 actgaaaccg gggtgggtta ccggtttcag gtataggctt cgacgaacac cggccgccat
    13741 gccgggacca cagggagtta tcgatgagtg atccatccag tcattccagg gcgggcctgc
    13801 tggccctgag cctcgcggcg ctgggggtcg tctatggcga catcggcacg agtccgctgt
    13861 acgcgttcaa ggaagcgttt tccggaccgc acgggatcga cccctcggag ggcaatgtgc
    13921 tggcggccct gtcggccttt ttctgggcgg tggcgctgat cgtctcggtg aaatacgtgg
    13981 gcatcgtgct gaggttcgac aacgaggggg agggcggggt gctggccctg acggcctttg
    14041 cccatcgggt gtcgagcaat ccgcgctgga gtgtgctggc ggtggctggc gggatcttcg
    14101 ccgcggcctt gttctatggc gacgcgatca tcacgccggc gatctcggtg ctgtcggcgg
    14161 tggagggcat cagcgtggcc accccgcacc tggagcacgc ggtggtgccc atcacggtgg
    14221 gcatcctggt ggcgctgttc ctgatccaga agcacgggac aggccacgtg gggcgtttct
    14281 tcgggccggt cacggtggtg tggttcctga ccctggcggt actgggtgtc gccagcatca
    14341 tggagacgcc ccgcgtgctg caggccatcg atccgcgcct ggccgtcgaa ttcgcgtttg
    14401 aaaagcccaa ggtcgccttc gtgctgctgt cggcggtgtt cctggccctc accggcggcg
    14461 aagcacttta cgcggacatg ggccactttg gcgcacggcc ggtgcgcctg gcctggtatg
    14521 gcctggtgtg gccctcgctg atgctcaact acttcggcca gggggcactt gtcctgcgtg
    14581 agccggtggc catcgacaat cccttctaca tgctcgcgcc cgactggttc ctgctgccct
    14641 tggtcgggct cgccaccctg gccacggtga tcgcgtccca ggccaccatc accggcgcct
    14701 attccatgac cctgcaggcc acccgtctgg gttacctgcc gcgtctgcgc atcctccaca
    14761 cctccgatac cgagcgcggc cagatctatg tacccagcgt gaactggctg atgctggtgg
    14821 cggtgctcgt gctggtgttc cagttccgct cctccggcgc gctggcggcg gcttacggca
    14881 tcgccgtatc cggcacgatg atcatcacca cgctgctcac cgccttcatc gtgtggatgt
    14941 cgccgacgcg ttcgcgggtg gggctgctgg cggtgctgct gctgttcggc ctgctggagc
    15001 tgatgttctt cggttccaac ctgaccaaga tcgccgaggg cggctggctg cccatggtgc
    15061 tgggtggcac gatcttcctg atgctcacca cctggaagca gggcagtctg ctgctggccc
    15121 gcaagcgctg ccagatcgac gtgcccatgg ccggcttcat caaggggccg ctgccggatg
    15181 tgccgcgggt ggcgggcacg gcggtgtatc tgctgtccga ttccgatctg gtgcccagcg
    15241 ccctgttcca caacctcaag cactacaagg tgctgcacga gcgcatcgtt ttcctccatg
    15301 tggccaacca ggatgtgcca cgcatcgacg acgaggagcg ggtggaggtg acccagctgg
    15361 cgccgtccat cttcagcgtg gaggccaagt acggcttccg cgaggagccc gacgtgccgg
    15421 cggcactggc cctggcagcc cgttacgacc tggagctgga gcccatgacg accacgttct
    15481 tcgtggcccg ctccaacgtg gtggacggtc cgggcgagct gccgcgctgg cgctgtgcgc
    15541 tgttcgcgtg gatggtgcgc cagtccgaag gggccggttc gtccttccat ctgccggcca
    15601 atcaggtcgt ggaactgggc acccaggtga tgctctaggg tgcccgccgc ccgggcggtt
    15661 cgtggccgcg caccggcggc catggccttc acggacgacc atgggctaaa atcaccggct
    15721 ctccccgtag ctgacaggat tcaccatgca tgcctccgcg aaagtccata tcgacgatgt
    15781 ccgcatcaag gagatcaagg aactggcgcc ccccgcccat gtgctgcggg aattccccgc
    15841 cacgcccatg gccgcggaca ccacgtttgc ggcacggagc gcgatccatc gcatcctctt
    15901 cggcgccgac gaccgcctgc tggtggtgat cggcccgtgc tccatccacg attacgaggc
    15961 agcgatggac tacgcccggc gtctcaaggc cgaggcggac cgtctcaaga acgatctgct
    16021 ggtggtgatg cgggtgtact tcgagaagcc gcgcaccacg gtgggctgga agggcctgat
    16081 caacgatccg catctggacg gcagtttcgc catcaacgaa ggcctgcgcc tggcgcggcg
    16141 cctgctgtgg gaggtgaacg agctgggtct gccggccggc accgagtttc tggacatgat
    16201 cacgcctcag tacatcgccg acttcatcgc ctggggcgcc atcggagcgc gcaccaccga
    16261 gagtcaggtc caccgtgagc tcgcctccgg actgtcctgc ccggtgggct tcaagaacgg
    16321 caccgatggc aacatccgca tcgccgtgga cgccatcaag gcggcccagt cgccccatca
    16381 tttcctgtcc gtcaccaagg cggggcactc ggccatcgtg tccacgtccg gcaacgagga
    16441 ctgccacctg atcctgcggg gcggcaaggg ccccaactat gatgccgcca gcgttgatgc
    16501 ggcctgcacg gagctggccg cgtccggcgt gcagggccgc ctgatggtgg atttctccca
    16561 cgccaacagc cgcaagcagc acaagctgca ggtggaggtg gcccgcgacg tggccgggca
    16621 gctggccgag ggcgaggacc gcatcatcgg cgtgatggtg gagtcccacc tcaaggaagg
    16681 ccgtcaggac ctgaagccgg gggtgccgct ggagtacgga cagagcatca ccgacgcgtg
    16741 catcggttgg gaggacagcg tgaccgtgct ggagatgctg gccgaaggcg tgcgtcagcg
    16801 tcgcatccgt cgcgccacgt ccgaataaac ggcgtgcggc actgatcggg gccgggtcgc
    16861 ctgagggcgc ccggcctttt tcatgggcgc gggaccggct ggatggagcc ggtcagacat
    16921 gttcgatggt ctggccaccg cggcgcttgg cgcggtacat gcgccggtcg gcgacctcca
    16981 gcaggctgtc ggcggtctgg gcgtcctcgg gatagagggc aatgccgatg ctggtgccca
    17041 gcggcgcact gccgaggcgg ccggcgacgg gctggccgag tgcgtggagg atcgtgcccg
    17101 ccatctggtc ggcgtccgag ggctgatcca gttcggacag caggaccacg aactcgtcac
    17161 tacccaggcg tgcgacggtg tcgcaggggc ggatgcaggc ccgcagccgg tccgccgtgt
    17221 cgagcaggac ccggtctccg acggaatggc ccgacagatc attgatccgc ttgaagtcat
    17281 ccagatcgat gaggatcacg cccaggcgct cgcgataacg ttgggcgcgc tcaatggcct
    17341 ggcccagccg gtccacgagg agccgtcgat tggggagtcc ggtgagcgca tcatggtagg
    17401 ccagatgacg gatgcgctcg gtgccccgga cccgttcggt gacatcgacc gcgatgccgt
    17461 gataaccgag gaagcagccg tgttcatcct gccgcggcgt gccgctgagc gtcagccagc
    17521 ggggggggtg atagccatcg cggacctcca ccatgaggtc ccggaacgcg ccgcggtcct
    17581 cgaagatctg gcggatctcg gtctgtcgcg cgtcggactg gacaccgggg cgggggtcct
    17641 gcgtccacca cggacgcccg atgcgggcgt cgaggatgcc gtccagtggc gcgtggcagg
    17701 cgtggacgtg caccagccgg tgcagcgcgt cggtctgcca gtagccgtcg ccactctggc
    17761 tgaccagttc ctggagctgt gtgccgagca tggtccggcg gacctcgccg gccacgcgct
    17821 cggtgatgtc ggtggcgacc cacaccacgc gcagggggcg cgcgtgcgga tcgagggccc
    17881 gacccgagac ctctgcggtg aagatgctgc cgtcacggcg gcgcaggcgg accacgtccc
    17941 ggtagacccg tccctggcag agggcgctca gggccgtgcg ggcgcgccgc cgctcggagg
    18001 agtcggccag ccagtgggcc aggcgacctt cggcgcgggc atgggtcgag cgctcgccca
    18061 ccagttcgat gaagcggcgg ttgtgatgga tgatgcgcga tccggtgcac agcacgatgg
    18121 cttcaccggc accatcgagg atcttttcct cgaagccgcc gacggcggcg gcagcgggcg
    18181 gactgctccg cggccggtgc gcggcgtgga tcagatcgtg taccgcgaag gcgaaggcgc
    18241 caaagaggat gagcccggtg ctcgcatggg gcatgttcgg gggcggcagg tctaccgtga
    18301 ggatgaacag cggcaggagc gtggccagca cgaacacggc gaaggcgctg cggctcgccg
    18361 acaggatggc tgcgccgcca agggtgattc cgccgagcag gaggaaaagg gtgagctggg
    18421 ccggtgtccc ggaggtgtac gggccgcgca ggggcaaggc gccccaggcc gcgccggcga
    18481 ctacggcgcc gaaggacagg aggtaatggc ccaggtcttc atccagcgcg gaggggccgc
    18541 tgccggtccg cacccggtgg gcaatgctgg cgaggccgag ctggccggcc gtgaccaacg
    18601 cccaccagac gatcaggggg cgtggcgcat ccaccgtcgg ccacagggcc accaacgcga
    18661 tcaggcacgc gagcagtccg gctgcaacca tgcggacggg gtatcgtccg gggaggcgga
    18721 catcgcgggg gccggccgcc gtgcaggcgg ccggagcggg agcgaagaga ccggcaagct
    18781 tcacgcgatg gacgtctggc atgggggcgg ggcctgatgg cgagaggtgc ttcctggcga
    18841 aagggggcgg ccgggggccg gcctgaatcc gtcatgctgc acgacggaaa aagacacccg
    18901 ataattatcg cggctttggg gaatgaggaa aatatgtcga aagtaatagt taaatttcat
    18961 gtcataagca atagtggctt gtgtgtgggt gtattcccat gattgaaaag aagtttccgg
    19021 gcgtgcgggg tgtgctgtga gcgctcccgg aggggccgcc cggaaagggc ggtttgcgcc
    19081 ctcgccgacg ggtcccttgc atttcggctc ggtggtggct gccgtcgggt cctatcttga
    19141 tgcccgttcc tcaggggcgg cgtggctgct ccggatcgaa gacgtggatg tcccgaggtg
    19201 cgtgccgggt gccgccgagg ggatcctgcg gaccctcgaa cggctcgggc tggagtggga
    19261 tgggccggtg ctctggcaga gcagccggac ggaggtctac cgggccgcgc tggagcgcct
    19321 gatggccgcc ggacgggtct tcggctgcat ctgcacacgg cgggcgctgg cggacgcatg
    19381 cctggcgcgg gacgggacgc gacgctactc gggaacgtgt cgcgatggtc tgccacccgg
    19441 ccggaccgct cgggccttcc gcctcagggt ggagcccggc tgcgtgcgct ttgtcgaccg
    19501 gatccagggg cccattgagg aggatgtggc ggccgatgtg ggtgacttcg tggtgctgcg
    19561 ggccgacgga tatttcgcct atcagctggc ggtggtggtg gatgacgcgg aggcgggcat
    19621 caccgacgtg gtgcggggcg ccgacctgct ggattccacc ccgcgtcagc tcctgctgca
    19681 gcgagccctc ggactcccgc agccggctta tgcccacgtt ccggtggtga ccaatggcgc
    19741 gggcgagaag ctctccaagc agaccctcgc ccaggccgtg gatggcctgg cgccaggagg
    19801 tgtggtggcg gatgccctgc gtttcctcgg gcatccgccg cccgcggcgc tcggcggcgc
    19861 agcaccggcc gagctcctgg cctgggcgca gggcatctgg tcgctcgccc gggtgccgtg
    19921 tgtccgcggc gcgcccatgc cgggctacgg atgaggggcg tccagcgccc tgggttcagc
    19981 gttcgagtcg agcccgcagt gcggacactt ccacgcgcag ccgacgccgc tgcagcgtct
    20041 ggagccagcg caggcgggcg cgcagcagtg ccagggggga ttcgacctgg gcgcgccagg
    20101 tctggacgaa ccacgcttcg agttgcgcgg cgccgtcgtg cagacgtagc gtcaggcctg
    20161 gcggcggcgc gcagacgaag ctggcatcac catcggcgct caccgcgatc cagccgcccg
    20221 cttcgatgcg caggacatcg ccttcggcaa ggcgatggtg cacctggaag acctggtcac
    20281 cgagccagcg aggcgcatcg gtgacgctca ggcgcccggc gcaggcgatg atgagggtgc
    20341 ccgcgggcag gtgggcatgg tggcgttgac cgccgcgcag gcggatcggg gcaggggcgt
    20401 ccatggcggg ttctccggaa agtgacgggt acagcttaag gatcgggcgc gtccaccggc
    20461 aggcacagga cgccgctttg tgtaccggtt cagcgggcag aattgcttgc tgtcatggtc
    20521 cgctttccgt caatctgtac ctgtgccgga ccgccgggtc gggcgaagat cgcgcatgga
    20581 ccgtatcgac actgccgaaa ccctctacct gcgtgtcgcg gggtactatc gccgggccat
    20641 ccgcagcggc gccctggccg cgggagaccg tctgccctcc ctgcgggcgc tgacccgtct
    20701 gcacggggtc agtctgtcca cggtggtgca ggcctgccac tgcctggagg acgaaggtct
    20761 gctggaagcc cgcccacggt ccggctattt tgtccgtgcg gcgcgtggca gcaccctgca
    20821 gccggtgcgt gagccggacg tcgggcgtcc tccgagcctc cccgatccgg ccgtctttgc
    20881 cgggatccac gccagggtct ccgatttcgt cgccaagtgc gaacggttcc cgacccacac
    20941 caatctggcc caggcctttg gcccgccgga ggtgtacccc ctggaggccc tgcgcaacgc
    21001 cgcgatccgt gccctgcggc gccatccgga ggtgctggtc aatccggtgc cgcccctggg
    21061 ggacgcgggc tttcgcagcg tgctcgcgcg gcgggccgtg gccgcgggta tgagtccggc
    21121 gcccgacgag atcctggtga cgcacggatg caccgaggcg ctgaatctgg ccctgcgcgc
    21181 cgtggcagcg cccggtgacg tgatcgcggt agagtcgccc gcctatttcg ggctcctgca
    21241 ggtcatcgag agcctgggct tgcgtgccct ggagattccc accagtccca gtcgcggcct
    21301 gtcggtggag gcgctggagc tggccatccg gacgcatccg gatctgcgtg cggtggtggt
    21361 ggtgccgaat ttccagaatc ccctgggctg cgtgatgccg gacggcgaga aggcgcgcct
    21421 ggtggaactc tgttcgggcg ccggcgtagc gctgatcgag gacgacatct acggcgccct
    21481 cgacgacgac gatcagagcc tgaaggcgtg caaggcctgg gaccgggatg gcagcgtgat
    21541 ccactgcgcg tccttccaca agaccctggc gcccggcatg cgggtgggct ggatgctggg
    21601 cgggcgctgg ctggatcgca tccgcatgct caagcatgcc cagagccgtc ccaatgacgc
    21661 cctggcccag ctggccatcg gcgagttcat ggccggcagc gcctatgacc ggcacctggt
    21721 acgcctgcgc cggcatcttc tggcgcagcg gacgcacatg gcagaggcca tcgccaccca
    21781 ttttccggcg ggcacgcgcc tcaatccgcc gggcggcggg atgttgctgt gggtcgagct
    21841 gccggccggg tgcgattcga tggcggtctt cgatgcggcg ctgcgcagcg gcatccgcgt
    21901 ggcgcccggc acgatgtttt ccaattccgg acgtttcgac catt
//
LOCUS       DAOQMM010000057        21807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_816_length_21807_cov_49.050294, whole genome shotgun sequence.
ACCESSION   DAOQMM010000057 DAOQMM010000000
VERSION     DAOQMM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21807
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_816_length_21807_cov_49.050294"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1162)
                     /locus_tag="PLH85_10400"
     CDS             complement(101..1162)
                     /locus_tag="PLH85_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNB65019.1"
                     /translation="MARSFVSKLAGALLAAGLSLAGSAHAALFSNMYVFGDSLSDVGN
                     DLAITGGLVPDPAYYSNGAVTGRFVNGYNYADRLASQLGLSLAPSVLGGNDYAYGGAR
                     STYARPDLAVLGALSFQQQVNQYLGNVGGVVDPNALYVLWIGANDMSDGFAQTLAKLA
                     GTGVLDTSFIGNAAANALTTLFTEYATLQALGARHFVIGNVPDLGLTPAVRDLAALFG
                     NPAIMSIGTAASQAFNNGLMANLFAYTAPGTSTTVFDSYGLLHDMTANPAAYGLTNVT
                     SGCYDGQPDGTPSPGASTVSECANPDGYIYFDSEHPSARAHQIGGDLLYVQVVPEPAA
                     MALSVTALGLMGLMRRRRV"
     gene            complement(1370..1723)
                     /locus_tag="PLH85_10405"
     CDS             complement(1370..1723)
                     /locus_tag="PLH85_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB65020.1"
                     /translation="MTPILRRGTTARYSDFTVHGGVVYCVEVPPAGAADASAQTAAML
                     ASLERLLVEAGSAKDRLLLATLYLTDMADYDAVNAVWDAWVPPGTAPARACVQVCRLA
                     DPAWKVEVAVQAARN"
     gene            1816..3336
                     /gene="ilvA"
                     /locus_tag="PLH85_10410"
     CDS             1816..3336
                     /gene="ilvA"
                     /locus_tag="PLH85_10410"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNB65021.1"
                     /translation="MSAQHPDYLQKILTAQVYDAAIETPLDLAPNLSARIHNRIFFKR
                     EDMQPVFSFKLRGAYNKMASLSPAALKRGVICASAGNHAQGVALSARKLGVRAVIVMP
                     TTTPAIKIDAVRRHGGEVVLAGESYSDCYLHAKELEKAEKLTFVHPYDDVEVIAGQGT
                     IGMEILRQHPKPIHAVFCCVGGGGLLAGVAAYIKRVRPDIKVIGVETVDANAMTQSLA
                     AGKRIELANVGLFADGAAVKLVGEETFRLCQAYVDEMILVDNDAICAAIKDVFEDTRS
                     ILEPAGALAVAGAKEYAKRHALKDKTLVAVASGANMNFDRLRFVAERAEVGEQREAVL
                     AVTIPEKPGSFKKFCNLLGNRNITEFNYRYADAHRAHIFVGVGVHNRAEGARLTELLQ
                     RHELPCLDLTDDEMAKSHIRYMVGGHAPQATNEVLYRFIFPERPGALLNFLETLRSDW
                     NISLFHYRNHGADFGRVLVGMQVPPADRDAFQGFLDKLGYEYVDETLNPAYRMFLG"
     gene            3365..5692
                     /locus_tag="PLH85_10415"
     CDS             3365..5692
                     /locus_tag="PLH85_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNB65022.1"
                     /translation="MIGLSERLQALRGRLRRPTRRELLRALVALPLLFVLWVLVLIPF
                     TPGIGDLRKAKQERPARVLSADGREIAVFKWANREWVGLADISPHVVKALIATEDHRF
                     YEHHGIDFYRLGGALLHTFSGDRQGGSTITQQLARNLYPDEIGRAPTLTRKLREAITA
                     LKIEAVYSKDEILETYLNTVPFLYNAWGIEMAARTYFDHSARQLDILESATLVGMLKG
                     NSAYNPVQNPERAVARRNVVLAQMARRGQLDPARLDALRKKPLRIDFERQVEAPGPAP
                     HFAQQVRKWLIAWADRNGYNLYADGLVVRTTLDSRLQVDATRAVARRGRQLQALADQA
                     WSGRKGWSGSPELVAALVRESRDYRNAVAAGQAPDAVLARLLRDTVFLNALRADKTRI
                     QAGLLAIDPRDGQVRAWVGSRDFVQDPFDHVQQARRQPGSTFKPFVYGAAFARGARPE
                     DTLDDVAVEIPVRGDEVWRPTDESPPSGRPVSLRDGLAYSRNRITAQLMQQVGPARVA
                     HLARALGVRQSPLEEVPSLALGTSPVTLREMVTAYAAIADGGHYREPLLVTQVEDADG
                     HILERIAPPAPEEALDPAVAYTLLDTLRGVIDRGTGNALRSRFGLRADLAGKTGTTQD
                     NTDGWFIAMHPQLVAGAWVGFNDSRVTLRSDYWGQGAHSALPIVGDLLHDAVAERLVD
                     GKARFVSQARPGLLTRAWDWLRDLFDRPEAPPAPPRKAPRAAPPAETPLPPPAGPELP
                     PPEDLAPPPAAAPAAAVPLPDTAPLPPPAEASARP"
     gene            complement(5703..6989)
                     /locus_tag="PLH85_10420"
     CDS             complement(5703..6989)
                     /locus_tag="PLH85_10420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB65023.1"
                     /translation="MATQGDEFPAEVGFAAIFAASPDGLLVVDGSGRIRLANPAVEHM
                     FGYAAGELIGQAIEVLVPAHAAGHHVRDRMAYAAAPRTRPMGASTELAGRCRDGSLVP
                     VDIMLAPVDATGTRGTLCVVRDARPRHATEAKLHLASAVFQTTQEAIVVTDTQCRILA
                     VNPAFERVTEYAEAEVLGQHMRIVASGRHDRSFYDAMWTSIHTTGEWQGAIWNRRRSG
                     EIYQEWLSISTVRDREGRAFQYVGVSADMTRMNHAETPIERLAHFDPLTGLPNRLLLD
                     SRIRKTWERAHRDGLRFAVLFLDLDGFKAVNDDRGHAVGDELLRVIAARLAGSVRESD
                     TVARLGGDEFIVVLEDLRREDVARLADKLIARVAEPVELSDGSQAHVGLSLGWSTYPD
                     DAQDIDTLMGQADTALYRAKRGGRGRAVAFVGSGPA"
     gene            7159..8049
                     /locus_tag="PLH85_10425"
     CDS             7159..8049
                     /locus_tag="PLH85_10425"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNB65024.1"
                     /translation="MICPSLPDTPSGFPLAIIGAGALGLHFAARLAGCGPVAVLARSA
                     ARAETLRAGLVVGDARFRPQAFGPDDPLRAEWVLLLVKTGDTEAAARQALHLQPHGIL
                     SLQNGLTESRLRAVCGSVPAAQGLTTEGAYRDSAGVHPAGAGETLLPPGFETLAAHLQ
                     AAGFRARIEPDMGPARLAKLLVNLAINPLTALHRVPNGALLDAPYRVELEALVDEAWP
                     VLHAAGLRLDRTAAHARVVTVAAATAGNRSSMLQDVLAGRPTEIDAITGAVLDMASAQ
                     GRDLPLNRTVFTRIKGLAPP"
     gene            8129..8983
                     /locus_tag="PLH85_10430"
     CDS             8129..8983
                     /locus_tag="PLH85_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65025.1"
                     /translation="MNESLQSWWFFLCAAATVNVLAWLMSAAMLRRGGPDLPERARAL
                     RRTQLLLSAGYVFGCAFRSVLPVFDVPRLCLVDSWLSSVVVGRSVATVAELCFVAQWA
                     LILREVARATHASFARRAAALMVPLIAVAETCSWVAVLTTANLGHVIEESLWGLSALL
                     FVLGVLETRPRCPPWLHGRLLLWGAAGLAYTVFMFGVDVPMYWSRWIADEHVGRAYLS
                     LTQGLTDASTRWVVSHRWEDWRSEVVWMSLYFSVAVWFSIALVHTRLPARVRLHSRSL
                     RSPAASRA"
     gene            complement(8997..9167)
                     /locus_tag="PLH85_10435"
     CDS             complement(8997..9167)
                     /locus_tag="PLH85_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65026.1"
                     /translation="MKRLLALALIAASLGGCVVLPPERGPRYMGPPPVVVAPGRPYHY
                     YDHDRDWGYRRW"
     gene            9292..10008
                     /gene="sfsA"
                     /locus_tag="PLH85_10440"
     CDS             9292..10008
                     /gene="sfsA"
                     /locus_tag="PLH85_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNB65027.1"
                     /translation="MRFTKPLLEGRLIRRYQRFLADVDTPAGPVTAHCPNTGSMLGCA
                     APGLRVWLSPAANPARKLAWTWELVEVPGDTAPVRVGVHTGRSNGLVREAIEAGRVPA
                     LAGYPTIRGEVKYGEGSRIDLLLSGDGRPDCYVEVKNVTAAVSGGVGYFPDAVTTRGA
                     KHLREMAAMVAVGRRAVLVFCVQRADVAAVRPADHIDPAYGRALREALAAGVEVLALG
                     ARVSPDEIVVDRALPVTVPA"
     gene            complement(10022..10975)
                     /locus_tag="PLH85_10445"
     CDS             complement(10022..10975)
                     /locus_tag="PLH85_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNB65028.1"
                     /translation="MHRIVVLEAGAVIATLRRPAFAHEWVEYAATRQEQVAERLAGAT
                     IAVVNKRQLGAELLAGLPTLRLIAIAATGYNNVDLEACRARGIVVCNVRGYARQTVPE
                     HVFALLLALARQIGPYHRSVAAGRWQRSEQFCYFDYPIRDLAGQTLGLLGAGSLGEGV
                     ARIAAAFGMHVLRGERKGAASVRDGYVAFDTLIRTADVISLHCPLTEATRHLIGAAEL
                     AAMKPTALLLNTARGGLVDEAALAEALRARRIAGAGFDVLSEEPPRDNPLLAPDLLEA
                     GRFLLTPHVAWSSREAMQALADQLMDNIDAFAAGTPRNRLA"
     gene            complement(11177..11809)
                     /gene="cobO"
                     /locus_tag="PLH85_10450"
     CDS             complement(11177..11809)
                     /gene="cobO"
                     /locus_tag="PLH85_10450"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNB65029.1"
                     /translation="MNEPTPPAPPAEPDRDARHTARMARKKAVVDAAIAAATERRGVL
                     LVNTGNGKGKSSAAFGVLARALGHGLQCGVVQFVKGRSDTGEEAFFRRQPEVRWHVMG
                     DGFTWDTQDRAADMASARAGWEKARAFLRDPAIGLVVLDELNIVLKYAYLDVAEVVAD
                     LRARPPQQHVIVTGRGAPAALIDVADTVTEMGPVKHAFQAGVAAQAGVEF"
     gene            complement(11874..12656)
                     /locus_tag="PLH85_10455"
     CDS             complement(11874..12656)
                     /locus_tag="PLH85_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB65030.1"
                     /translation="MKMDVRPDVLAAEALAVQVGRRDVVRGLEVRVAPGDTLVILGRN
                     GAGKTTLLHTLAGLRPAAGGTVLLSGKPYADWPRGHEACLRGLLPQSQPDFFSATVLE
                     TALVGRHPHLGRWAWEGPDDEAIALAALATVGLAEYAARPVLTLSGGERQRLAIAALL
                     VQQPRLYLLDEPLAHLDLHHQIGVLDHFHRLAADGAGVVMVLHDINLAARYASHVLLL
                     DGAGGFACGPARDILTTARLSAAFGHPLRAVADGASTLFVPA"
     gene            complement(12653..13636)
                     /locus_tag="PLH85_10460"
     CDS             complement(12653..13636)
                     /locus_tag="PLH85_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNB65031.1"
                     /translation="MGRVRQRAALVLLVLVAAALAAFAWALSAGELPVTAADLLAALG
                     GGAGSMEADIIRTLRLPRAVAAFACGGLLALSGALMQVLLRNPLADPYVLGVSGGAAV
                     GALGCLLAGVAGLWIDVGAFAGALVATLLVFGLARGDGSWTRTRLLLTGVIVAAGCGA
                     AVALMLSIAPEGRVKGMLFWLMGDASGVSNPWPALGVLAVGLLTALPFARELNLLARG
                     EVTARALGVRVGRLRALVYGLGALLTGVAVTTLGSVGFVGLIVPHLVRLVIGNDQRLL
                     LPAASLAGGVLLTLADTAARTVVAPVQLPVGVLTALIGVPVFLFLLARHPR"
     gene            complement(13700..15556)
                     /locus_tag="PLH85_10465"
     CDS             complement(13700..15556)
                     /locus_tag="PLH85_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB65032.1"
                     /translation="MTIRPTLAALAVAAAFPTVFSSAAFAASNAPAAAAVSSAEQPVV
                     LVTATRQTQRADESLADVTVIERARIEEAGASTSLAELLARQPGIQMSSSGGPGKATD
                     VYIRGASPKHSLLIVDGVRLGSATLGASSWQDIPLTQIERIEIVRGPASALYGADAIG
                     GVIQIFTRRGEGAPKADAFVGVGSYGNKEVAAGVSGGTERLSYSLRGAYTDVAGYNAI
                     ASQAKQPYYYNADKDPYRNSSLSGNLAYTLMPGHELGATLLYATARNHYDSGAGYDDF
                     ADNVQSVWSAYSRNRFSAGWTSTLRIGQSIDDARTYASYAPTGATIKTTQDQVMWQND
                     ITLPLGTLLAALENLEQKAVNEGTFSVSRQIRSALLGWTASVGEHRMQFNARYDDNSQ
                     FGNKTTGYAGYGYQILPSLRVQGSVGSAFRAPTFNELYFPGFGNPNLKPENAYNKELG
                     LLWEQHGQSVSLTAFHNRITNLIAYDANFIPQNVNNARLQGVSLGYAGTLAGFDINAN
                     LDFLDPEDADTGKRLQRRADKTGNLYLSRRIAAFNVGAEWQGAAGRYDRTGEINRMGG
                     YGLVNAFVKYAVDRDWSLEARANNLLNKQYETAWGYNTAGANLFFGVRYAPR"
     regulatory      complement(15676..15917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            16007..16555
                     /gene="cobU"
                     /locus_tag="PLH85_10470"
     CDS             16007..16555
                     /gene="cobU"
                     /locus_tag="PLH85_10470"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNB65033.1"
                     /translation="MSPELILGGARSGKSRHAEHLAAASGLPVTVIVTAEALDAEMAA
                     RIARHRTDRPAGWATVEAPRDLAGALVATAAHGRCTVVDCLTLWLANLLDGAEHLPPA
                     TPAEALPAFARERAAFLDALPTLPGRVILVANEVGLGLVPETPLGRLFRDEAGRLNQA
                     VAALCGRVTFVAAGLPLVLKGG"
     gene            16630..17667
                     /gene="cobT"
                     /locus_tag="PLH85_10475"
     CDS             16630..17667
                     /gene="cobT"
                     /locus_tag="PLH85_10475"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNB65034.1"
                     /translation="MHYTLIRPDRGLAPALQQRIDTKTKPLGALGRLEALALQIGLIQ
                     QTLEPELRQPHVMVFAGDHGAARAGVSAYPQDVTWQMVENFLAGGAAINVFARQAGMG
                     LSVVDAGVAHDFGPRPGLIAAKVAPGTANYLEAPAMDAAQRNAALAHGRELALGLAGT
                     GCNVVGFGEMGIGNTAAASLLTHCLTGADLATVIGRGTGLDDAGLARKRELLAHAVAR
                     GGRPADPLDALAQYGGFEIAMMAGAMLGAAEARMVLLIDGFIVTSALLVAHALAPAVL
                     DYCVFAHRSQEPGHAVQFDHLGAEPLVQLDLRLGEGTGAALAYPLVQAAVNFLNEMAS
                     FESAGVSGKAD"
     gene            17673..18443
                     /locus_tag="PLH85_10480"
     CDS             17673..18443
                     /locus_tag="PLH85_10480"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNB65035.1"
                     /translation="MRTQLELFLTAVGFFTRLPVPGWVPFSVERLAHAARYLPAVGWL
                     VGGLGALAYGLASQVLPPDVAVVLSMVATLVATGAFHEDGLADTCDGLGGGLDRDRAL
                     AIMKDSRIGSYGAIGLVLALLLKALALVNLGVDADMPVILALLAAHPLSRLAPVTLMA
                     VLDYARTGHDAKAGAVARRLTPAELGIATLAGVLPLLLLDATEAGLALAATVIVTAWA
                     GRLFRRRLDGYTGDCLGATQQIAELAIYLALLAAWNFT"
     gene            18428..18985
                     /gene="cobC"
                     /locus_tag="PLH85_10485"
     CDS             18428..18985
                     /gene="cobC"
                     /locus_tag="PLH85_10485"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HNB65036.1"
                     /translation="MELHLIRHPRPDVAPGVCYGQTDVGLAESAAAVAARLRPLLPAG
                     CALIASPLRRARLLAEALGTPQLDPRLMEIHFGDWEGQRYADLGSALDAWAAAPLDFA
                     PPGGETPRQMAARVLDFLHDLQATPPAAAVAIVAHGGPLRVIAGHLLGLPDTHWLGLD
                     FACGEATRIDCHDWGTVLRWFNRRA"
     gene            19146..19778
                     /locus_tag="PLH85_10490"
     CDS             19146..19778
                     /locus_tag="PLH85_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB65037.1"
                     /translation="MNRPFRPALLAAAIAAAVPAHAYVATAFDASVYTANTAALDSAL
                     GLTGIVAIEDFEDGVLIPGLTSNLTTYGASLPNHAWDGLTASTWGNALFTVSLTGIRQ
                     FGIGISDNDGGGQTVTVNGHFTLDLSTVAGYRGDTSGRATYVLIHAESGDADITRIAI
                     DNSLTPLFDHLVLSTDSGLAPSALPEPSSAALMLAGTALAGWLRGRNRMK"
     gene            19905..20333
                     /locus_tag="PLH85_10495"
     CDS             19905..20333
                     /locus_tag="PLH85_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65038.1"
                     /translation="MNSIRSGPIRRPPKPITWWHALAGVLVGSLLATLLAWMLPRTDA
                     EELLLPVHATYAARPARGDGLPAGLSLRGIIPPSGDSPGIALLKEPGRPVVLVTEGHE
                     YAPDLRVVRVLQDRILIRQRGNGDDLILTLVDPSSGSPVP"
     gene            20470..21126
                     /locus_tag="PLH85_10500"
     CDS             20470..21126
                     /locus_tag="PLH85_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNB65039.1"
                     /translation="MGVSAMMFRASFQHALSAVLVTAALTGTATAATVTPSSYTYDQS
                     TACGTWCYHDPANTKLTDGVLGLAGWAVNAGNEWDGWVYQNRVNIDFDFGTSRHIDLV
                     RVGTTQDNLGDVVLPSIELYISNDKSTWTFVTGLTVPPDSAHNIDPFSTAPHDFLTLA
                     GLGIDARYVRVGAIANGPWIFVDEVQFQSRTTQPVPEPAALGLLAAGLASLAGTRRPR
                     "
     gene            complement(21164..21805)
                     /locus_tag="PLH85_10505"
     CDS             complement(21164..21805)
                     /locus_tag="PLH85_10505"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HNB65040.1"
                     /translation="MPRAPGREEEMDEEGLVPFSGLIRYERHVPIHQVSYYLCGEIRE
                     PQYYTELFYTLRTASATDLVYLHINSPGGDFNTGLQIINNMLASEARVVTVLEARAYS
                     MAAFVFLSGDELIVHDNCQLMFHTYSGSFAGKGNEQLAEVQALGAWFEKVTTRICSPF
                     LSAADIDRILKGSDLWMDSDEIRHRLGRKGRPAGTRRGTRTAAKRLPDVPSAD"
BASE COUNT         3231 a         8038 c         7322 g         3216 t
ORIGIN      
        1 ggaccgtgtc gccgcgacaa agttgtgccc ccgctcaagc gaccgcgcac catgaaaaaa
       61 cccgccacga gggcgggtct gcacggcgcc tggaagggaa tcagacgcga cggcggcgca
      121 tcaggcccat caggccgagg gcggtgacgg acagcgccat cgcggccggc tccggcacca
      181 cctggacgta gagcagatcg cccccgatct ggtgcgcccg cgcgctgggg tgctcggaat
      241 cgaagtagat gtagccgtcg gggttggcgc actcgctcac cgtggacgct ccgggcgaag
      301 gcgtgccgtc gggctgaccg tcgtagcagc ccgaggtcac attggtgaga ccgtaggccg
      361 ccgggttggc ggtcatgtca tggagcaggc cgtaggaatc aaagacggtc gtgctcgtcc
      421 caggcgccgt gtaggcgaac aggttcgcca tcagcccgtt gttgaacgcc tggctcgcag
      481 ccgtcccgat gctcatgatg gcagggttgc caaacagcgc cgccagatcg cgcacggccg
      541 gcgtcaagcc caggtcggga acgttgccga tcacgaagtg tcgggccccc agggcctgca
      601 gcgtggcgta ctcagtgaac agcgtggtca gggcattcgc cgccgcattg ccgatgaagg
      661 acgtatcgag cacccccgtc cccgccagct tggcgagggt ctgagcgaag ccatcggaca
      721 tgtcgttggc gccaatccac agcacgtaga gggcgttggg atcgaccacg ccgccgacgt
      781 tgccaaggta ctgattcacc tgctgctgga agctgagcgc acccagcacg gccagatcgg
      841 gacgcgcata ggtgctgcgg gcaccgccat aggcgtaatc gttgccgccc agcacggacg
      901 gcgcaaggct caggcccagt tgcgaggcca gacggtccgc gtagttgtaa ccattcacga
      961 aacgccccgt cacggcgcca ttcgagtagt aggccgggtc gggcaccaga ccgccggtga
     1021 tcgccagatc gttgcccaca tccgacaggc tgtcgccgaa gacgtacatg ttgctgaaga
     1081 gcgccgcgtg cgcggacccg gccaaactga gcccggcggc gagcagtgcg ccggcgagct
     1141 ttgaaacgaa tgaacgcgcc atgaaaacct ccctgattga agtcgactgc ccgccgcgac
     1201 gcatgcaatc cagggcccct ccgccctgtc gcccacctat tgccatgccg cggccgccgt
     1261 gaaaaagttc acgcccccat cttatgcgct gcaaatgaaa aattcgtgca ctttttaacg
     1321 ttttcccgat gattcgctgc ttgacgtcac gcaagttttc aacaccgatt cagttccttg
     1381 ccgcctgtac ggccacctcc accttccagg ccgggtcggc caggcggcac acctgcacgc
     1441 aggcccgcgc cggcgccgtg ccgggcggca cccaggcgtc ccacactgca ttcacggcgt
     1501 cgtagtcagc catgtcggtg aggtacaggg tggccagcag gaggcggtcc ttggcgctgc
     1561 ccgcctcgac gagcaggcgt tccaggctgg ccagcatcgc ggcagtctgg gcggacgcgt
     1621 cggcggcgcc cgctggcggc acctcgacgc aatacaccac gccgccgtgg acggtgaaat
     1681 cggaatagcg ggcggtcgtg cccctgcgca ggatcggggt catgggggat ctccaagcaa
     1741 aaagccgtat gttgcagcgc agccggtccc gctgctaccc tcccaggctt cccccagccg
     1801 aaacgcaggt ttgccatgag cgcgcagcac cccgactacc tccagaagat cctcaccgcc
     1861 caggtctacg acgcggccat cgagacgccc ctcgacctgg cgcccaatct gtcggcgcgc
     1921 atccacaacc ggatcttctt caagcgcgag gacatgcagc cggtgttcag cttcaagctg
     1981 cgcggggcct acaacaagat ggccagcctc agcccggcgg ccctcaagcg cggcgtgatc
     2041 tgcgcctccg ccggcaacca cgcccagggc gtggccctgt cggccaggaa gctcggcgtg
     2101 cgcgccgtga tcgtcatgcc caccaccacg ccggccatca agatcgacgc ggtccggcgc
     2161 catggcggcg aggtggtgct ggccggcgag agctactccg actgctacct gcacgccaag
     2221 gagctcgaga aggccgagaa gctcaccttc gtccatccct atgacgacgt ggaagtgatc
     2281 gccggccagg gcaccatcgg catggagatc ctgcgccagc atcccaagcc catccacgcg
     2341 gtgttctgct gcgtcggcgg cggcggcctg ctggccggcg tggcggccta catcaagcgg
     2401 gtgcggccgg acatcaaggt gatcggcgtg gaaaccgtgg acgccaacgc catgacccag
     2461 tccctggccg ccggcaagcg catcgaactg gccaatgtgg gcctcttcgc cgacggcgcg
     2521 gcggtcaagc tggtgggcga ggaaaccttc cgcctgtgcc aggcctacgt ggacgagatg
     2581 atcctggtgg acaacgacgc catctgcgcc gccatcaagg acgtcttcga ggacacccgc
     2641 tccatcctcg agcccgccgg cgcgctggcg gtagccggcg ccaaggaata cgccaagcgc
     2701 cacgccctca aggacaagac cctggtggcg gtggcctccg gcgccaacat gaacttcgac
     2761 cgcctgcgct tcgtcgccga gcgcgccgag gtgggcgagc agcgcgaagc cgtgctggcc
     2821 gtcaccatcc ccgagaagcc cggctccttc aagaagttct gcaacctgct cggcaaccgc
     2881 aacatcaccg agttcaacta ccgctacgcc gacgcccacc gtgcccacat cttcgtcggc
     2941 gtgggcgtcc acaaccgggc cgaaggtgcg cgcctcaccg aactcctgca gcgccacgag
     3001 ctgccctgcc tcgacctcac cgacgacgag atggccaaga gccacatccg ctacatggtc
     3061 ggcggccatg cgccgcaggc gaccaacgag gtgctgtacc gcttcatctt cccggagcgc
     3121 cccggcgccc tgctcaactt cctggaaacc ctgcgctccg actggaacat cagcctgttc
     3181 cactaccgca accacggtgc cgacttcggg cgcgtgctgg tgggcatgca ggtgccgccc
     3241 gccgaccggg acgccttcca gggcttcctc gacaagctcg gctatgaata tgtggacgag
     3301 actctgaacc cggcctaccg gatgttcctg ggctgaggcc cgccgcggcc ggcgagctga
     3361 ccacgtgatc ggcctttccg aacgcctcca ggccctgcgc gggcggctgc gccggcccac
     3421 ccggcgcgag ttactgcggg ccctggtggc gctgccgctg ctcttcgtgc tgtgggtgct
     3481 ggtgctgatc cccttcaccc ccggcatcgg cgatctgcgc aaggccaagc aggagcgtcc
     3541 ggcacgggtg ctgtcggcgg acggtcgcga gatcgcggtg ttcaagtggg cgaaccggga
     3601 atgggtcggc ctggcggaca tctcgcccca cgtggtgaag gccctcatcg ccaccgagga
     3661 ccaccgcttc tacgaacacc acgggatcga cttctaccgc cttggcggcg cgctgctgca
     3721 tactttctcc ggcgaccgcc agggcggctc gacgatcacc cagcagctcg cccgcaacct
     3781 ctacccggac gagatcggcc gcgcgcccac cctcacccgc aagctgcggg aagccatcac
     3841 cgccctcaag atcgaggccg tctacagcaa ggacgagatc ctcgagacct acctcaacac
     3901 ggtgcccttc ctctacaacg cgtggggcat cgagatggcc gcgcgcacct acttcgacca
     3961 ctccgcgcgc cagctcgaca tactcgagag cgccaccctg gtgggcatgc tcaagggcaa
     4021 cagcgcctac aacccggtgc agaacccgga gcgggcggtc gcccgccgca acgtggtgct
     4081 ggcccagatg gcgcgccgcg gccagctcga tccggcacgc ctcgacgcgc tccggaagaa
     4141 gcccctgcgc atcgacttcg aacgccaggt cgaagccccc ggcccggcgc cccacttcgc
     4201 ccagcaggtg cgcaagtggc tgatcgcctg ggccgaccgc aacggctaca acctctatgc
     4261 cgacggcctg gtggtgcgca ccaccctcga tagccgcctg caggtcgacg cgacccgcgc
     4321 ggtggcgcgt cgcggccgcc agttgcaggc actggccgac caggcgtgga gcggacgcaa
     4381 gggctggtcc ggcagccccg agctggtcgc cgccctggtg cgcgagagcc gcgactaccg
     4441 caacgccgtc gccgccggcc aggcaccgga cgcagtgctc gcccgcctgc tccgcgacac
     4501 cgtcttcctc aacgccctgc gcgccgacaa gacccgcatc caggccggcc tgctggccat
     4561 cgacccgcgc gacggtcagg tccgcgcctg ggtcggcagc cgggatttcg tccaggaccc
     4621 cttcgaccac gtgcagcagg cccgccgcca gcccggctcc accttcaagc ccttcgtgta
     4681 tggcgccgcc ttcgcccgcg gcgcgcggcc cgaggacacc ctcgacgacg tggcggtgga
     4741 gatccccgtg cgcggcgacg aggtctggcg ccccaccgac gaatccccgc ccagcggccg
     4801 cccggtcagc ctgcgggacg gcctcgcgta ctcccgcaac cgcatcaccg cccagctcat
     4861 gcaacaggtc ggcccggcgc gggtggcgca cctcgcccgc gccctgggcg tgcgccagag
     4921 tccgctggag gaagtgccgt ccctggccct gggcaccagc cctgtcaccc tgcgggagat
     4981 ggtcaccgcc tacgccgcca tcgccgacgg cggccactac cgggagccgc tgctggtcac
     5041 ccaggtggaa gatgccgacg gccacatact ggagcgcatc gccccgccag cgcccgaaga
     5101 ggccctcgac ccggccgtcg cctatacgct gctcgacacc ctgcgcggcg tcatcgaccg
     5161 gggcaccggc aatgccctgc gcagccgctt cggcctgcgc gccgatctgg caggcaagac
     5221 cggcaccacc caggacaaca ccgacggctg gttcatcgcg atgcatccgc agctggtggc
     5281 cggtgcctgg gtcggcttca acgacagccg cgtcaccctg cgcagcgact actggggcca
     5341 gggcgcccac agcgccctgc ccatcgtcgg cgacctgctc cacgacgccg tcgccgaacg
     5401 actcgtggat ggcaaggcgc gctttgtctc ccaggcccga ccgggtctgc tgacgcgcgc
     5461 gtgggactgg ctgcgggatc tgttcgatcg ccccgaggcg ccgcctgcgc cgccgcgcaa
     5521 ggcaccgcgc gccgcgccgc cggcagaaac gccactgccc ccgccggcgg ggcccgagct
     5581 gccaccgccg gaggatctcg cgcctccgcc tgcggccgcg cctgccgctg cggtacccct
     5641 tccggacacc gcgccgcttc caccgcctgc cgaggcgagc gcgcgccctt agagaccgtc
     5701 gatcaggccg gccccgaccc gacgaaggcg accgcccggc cgcgaccgcc ccgcttggcc
     5761 cggtacaagg ccgtatcggc ctgacccatc agggtgtcga tgtcctgggc gtcgtccgga
     5821 taggtgctcc agccaaggct gagccccacg tgggcctggg agccgtcgct cagctccacc
     5881 ggctcggcca cccgcgcgat cagcttgtct gccagccgcg ccacatcctc gcggcgcaga
     5941 tcctccagca ccacgatgaa ctcgtcgcca cccaggcgcg ccaccgtatc cgattcccgc
     6001 accgagccgg ccaggcgggc ggcgatcacg cgcagcagct cgtcaccgac agcatgcccc
     6061 cgatcatcat tcacggcctt gaagccgtcc agatcgagga acagcacggc gaaacgcagc
     6121 ccgtcccggt gcgcccgctc ccaggtcttg cggatgcgcg agtccagtaa caggcggtta
     6181 ggcagacccg tcagggggtc gaagtgggca aggcgctcga tgggcgtctc ggcgtggttc
     6241 atccgcgtca tgtcggcgct cacccccacg tactggaagg cgcgcccttc ccggtcgcgc
     6301 accgtgctga tggacagcca ctcctggtag atctccccgc tcctgcgccg attccagatg
     6361 gcgccctgcc attcgccggt ggtgtggatg gacgtccaca tggcgtcgta gaacgatcgg
     6421 tcgtggcggc ccgaggccac gatgcgcatg tgctgcccca gcacctccgc ctcggcgtat
     6481 tcggtgaccc gctcgaaggc cggattgaca gcaaggatgc ggcattgcgt atcggtgacg
     6541 acgatcgcct cctgggtcgt ctggaacacc gcgctggcca ggtggagctt ggcctcggtg
     6601 gcatggcgcg gccgggcatc gcgcaccacg cagagggtgc cccgcgtgcc cgtggcatcc
     6661 accggcgcca gcatgatgtc caccggcacc aggctgccgt cgcgacaacg tccggcgagc
     6721 tcggtcgatg cacccatcgg ccgcgtccgc ggcgccgcgg cgtaggccat gcggtcccgg
     6781 acatggtgac ccgcggcgtg cgccggcacc agcacctcga tggcctggcc gatcagttcg
     6841 cccgcggcgt aaccgaacat gtgctccacc gcagggttgg ccagacggat ccggccacta
     6901 ccgtccacca ccagcagccc atccggcgac gccgcaaaaa tcgcggcaaa accgacttcc
     6961 gcgggaaatt catccccttg cgtcgccatg gcagaatcct ccttcctcac cgcgcaccga
     7021 tccccgcttt gcctgtcccc ggagggcgtc caatcgcacc tggaccgcta ctgtaactgc
     7081 ttcggaacct gcccggacac ccccactgaa gcgctgggcg ggggacaatc cggaccgtcg
     7141 cccccgatcg cccgcgccat gatctgccct tccctccccg acacgccatc aggatttccc
     7201 ctcgccatca tcggcgcggg tgcgctcggc ctgcactttg cggcccgcct cgccggctgc
     7261 ggtccggtcg cagtcctggc ccgaagcgca gcccgggccg agaccctgcg tgcggggctt
     7321 gtggtgggcg atgcccgctt ccggccccag gccttcggcc cggacgaccc gctccgcgcc
     7381 gagtgggtgc tcctgctggt caagaccggc gacaccgagg cggcggcccg gcaggccctc
     7441 cacctgcagc cgcacggcat cctgtccctg cagaacggcc tcaccgaatc gcgcctgcgt
     7501 gccgtctgcg gcagcgtgcc cgccgcccag gggctcacca ccgaaggtgc ctaccgggac
     7561 agcgccggcg tgcatcccgc cggcgcaggc gaaaccctgt tgccaccggg cttcgagacg
     7621 ctggccgccc atctgcaggc cgccggcttc cgcgcccgca tcgagccgga catgggcccc
     7681 gcccgccttg ccaagctgct cgtcaacctc gccatcaacc cgctcacggc gctccaccgg
     7741 gttcccaacg gggccttgct ggatgcgccc taccgggtcg aactggaagc tctggtggac
     7801 gaagcctggc ccgtgctcca cgctgccggg ctgaggctgg accggaccgc cgcccacgcg
     7861 cgggtggtaa cagtcgccgc agccaccgcc ggcaaccgct ccagcatgct gcaggacgtc
     7921 ctcgccggac ggcccaccga aattgacgcc atcaccggcg cagtcctcga catggcgtcc
     7981 gcgcaaggcc gcgacctgcc cctcaaccgg accgtcttca cgcggatcaa ggggctcgcc
     8041 ccgccctgag cacgctgccg cacaaagcgc atacggtatt accgaattgc cttgacggcc
     8101 cgcttcgggc gacctagctt gaagcatcat gaacgagagc ctgcagtcct ggtggttctt
     8161 cctgtgtgcc gccgcgacgg tcaacgtgct cgcctggctg atgtcggccg cgatgctgcg
     8221 ccgcggcggt ccggacctgc ccgagcgggc ccgcgccctg cgccggaccc agttgctcct
     8281 gtcggccggt tacgtcttcg gctgtgcctt ccgctccgtg ctgccggtct ttgacgtgcc
     8341 acgcctgtgc ctggtggaca gctggctgtc cagcgtggtg gtggggcgca gcgtggccac
     8401 ggtggcagag ctgtgcttcg ttgcccagtg ggccctgatc ctgcgcgagg tggcccgcgc
     8461 cacccatgcc tccttcgcgc gccgggcggc ggcgctgatg gtgccgctga tcgccgtggc
     8521 ggaaacctgc tcgtgggtcg ccgtgctgac caccgccaac ctgggccacg tgatcgagga
     8581 gagcctgtgg ggcctgagcg ccctgctctt tgtgttgggc gtgctcgaaa cccgtccgcg
     8641 ctgtcccccc tggctgcacg ggcgcctcct cctgtggggt gcggcgggtc tggcctacac
     8701 cgtcttcatg ttcggcgtgg acgtgcccat gtactggtcg cgctggatcg ccgacgagca
     8761 tgtgggccgc gcctacctga gcctcaccca gggcctgacg gatgcctcca cgcgctgggt
     8821 ggtgtcccat cgctgggaag actggcgcag cgaggtggtg tggatgtccc tctacttcag
     8881 cgtggcggtg tggttcagca ttgccctggt ccacacccgt ctgccggccc gcgtgcgcct
     8941 gcacagccgc agcctgcggt cgcccgccgc gtcgcgtgcc tgacgctcga agccgctcac
     9001 cagcggcgat aaccccagtc ccggtcgtgg tcgtagtagt ggtagggccg gccgggggcg
     9061 accaccaccg gcggcgggcc catgtagcgc gggccgcgct ccggcggcag gaccacgcag
     9121 ccccccagtg aggcagcgat cagggcaagg gccagcaggc gtttcatgac gatctccggt
     9181 cggtgatgaa ggtgagcgtt caacgcacga gcccggcgcc aggttgggat gaaagggtta
     9241 ccgcgtgtaa gtctggcatg atgggcggat cgtcccgccg ccccacgccc catgcgcttt
     9301 accaagccgc tcctcgaagg ccgcctgatc cgccgttacc agcgctttct cgccgacgtg
     9361 gacacgccgg ccggcccggt caccgcccac tgccccaaca ccggctccat gctgggctgc
     9421 gccgcgcccg gcctgagggt gtggctgtcg cccgccgcca acccggcccg caagctggca
     9481 tggacctggg agctggtgga ggtgcccggc gacaccgcgc cggtgcgggt gggcgtacac
     9541 accgggcgca gcaacggcct ggtgcgggag gccatcgagg cgggccgggt gccggcactg
     9601 gcgggctacc cgaccattcg cggcgaggtg aagtacggcg aaggcagccg tatcgacctc
     9661 ctcctgagcg gcgacggccg gccggactgc tacgtggagg tgaagaacgt cactgcggcg
     9721 gtgagcggcg gcgtcggcta ctttcccgac gcggtcacca cccgcggcgc caagcatctg
     9781 cgcgagatgg ccgccatggt ggccgtcggc cggcgcgcgg tgctggtctt ctgcgtgcag
     9841 cgggcggacg tggcggcggt gcgcccagcg gaccacatcg acccggccta cgggcgcgcc
     9901 ctgcgcgagg ccctcgctgc gggcgtcgag gtgctcgccc tcggtgcgcg ggtgtcgccg
     9961 gacgaaatcg tggtggaccg ggcgctgccg gtgacggtgc cggcctgacg cagcgtccgc
    10021 ctcaggccag ccggttgcgc ggcgtgcccg cggcaaaggc gtcgatgttg tccatcagct
    10081 ggtcggccag ggcctgcatg gcctcccggc tggaccaggc cacatggggc gtgaggagga
    10141 agcggccggc ttccagcagg tccggcgcga gcaggggatt gtcccgcggc ggctcctcgc
    10201 tgagcacgtc gaagccggca ccggcgatgc gccgggcgcg cagggcctcg gccagggccg
    10261 cctcgtccac cagcccgccg cgggcggtgt tgaggagcag cgccgtcggc ttcatggcgg
    10321 ccagttcggc cgcgccgatg agatgccggg tggcctccgt gagggggcag tgcaggctga
    10381 tcacgtcggc ggtgcggatc agcgtgtcaa aggccacgta gccgtcgcgc acgctggcgg
    10441 cgcccttgcg ctcgccccgc agcacgtgca tgccgaaggc tgcggcgatg cgtgccacac
    10501 cttcccccag gctgccggcg ccgagcaggc cgagggtctg tccggccaga tcgcggatcg
    10561 ggtagtcgaa gtagcagaac tgctcgctgc gctgccagcg cccggccgcg accgagcggt
    10621 gataagggcc gatctgccgc gccagggcca gcagcagggc gaagacgtgc tcgggcacgg
    10681 tctggcgggc gtagccacgg acgttgcaaa cgacgatgcc ccgcgcgcgg caggcctcca
    10741 gatccacgtt gttgtagccg gtggcagcga tggcgatcag acgcagcgtg ggcaggccgg
    10801 cgaggagctc cgcaccgagc tggcgcttgt tcaccaccgc gatggtggca ccggccagac
    10861 gctcggccac ctgctcctga cgcgtcgccg cgtattcgac ccattcgtgg gcaaaggccg
    10921 ggcggcgcag ggtggcgatg acggcgccgg cttcaaggac gacgatgcga tgcatgggtc
    10981 gacaggcagg ttcgggagct gcgcagctta acccagccgg agcgcggccg ggcggctgcc
    11041 catgcgggcg gatccttcag cctgcggcgg ccgcgggccg tccgcccgtg caagcggctg
    11101 aaggtccatc caccggcctc gtctacgccc gccctgcacg caggccggga cgcagccgga
    11161 tgccgaggcg ggcccttcag aactccacgc ccgcctgggc cgccacgccg gcctggaagg
    11221 cgtgcttgac cgggcccatc tcggtcaccg tgtcggccac gtcgatcagc gccgcgggcg
    11281 ccccgcggcc ggtgacgatg acgtgctgct gcggcggccg ggcgcgcagg tcggcgacga
    11341 cctcggccac gtccaggtag gcgtacttga ggacgatgtt gagttcgtcc agcaccacca
    11401 ggccgatggc cggatcgcgc aggaaggcgc gggccttttc ccagccggcg cgggcgctgg
    11461 ccatgtcggc ggcgcggtcc tgggtgtccc aggtgaagcc gtcgcccatc acgtgccagc
    11521 gcacctcggg ctggcggcgg aagaaggcct cctcgccggt gtccgagcgg cccttcacga
    11581 actgcacgac gccgcactgc aggccgtggc cgagggcccg ggcgaggacg ccgaaggcgg
    11641 cgctggactt gcccttgccg ttgccggtgt tcaccaggag cacgccgcgc cgttcggtgg
    11701 cggcagcgat ggcggcatcc accacagcct tcttgcgggc catgcgggcg gtgtggcggg
    11761 cgtcgcggtc gggctcggcg ggtggagcgg gcggcgtcgg ttcattcatg ggcaggactc
    11821 gggaatcgct aaaagatagc gggggccgtt ggccgcactg cggcgaaaag cgctcaggcg
    11881 ggcacgaaca gggtgctcgc tccgtccgcg acggcccgca ggggatgacc gaaggcggcc
    11941 gagaggcggg ccgtggtgag gatgtcgcgg gccggtccgc aggcaaagcc accggcaccg
    12001 tccagcagca gcacgtgact ggcgtagcgg gcggccaggt tgatgtcgtg caacaccatc
    12061 accacgcccg caccgtcggc ggcgaggcgg tgaaagtgat cgagcacgcc gatctgatga
    12121 tgcaggtcca ggtgtgccag gggctcgtcg aggaggtaca ggcgcggctg ctggaccagc
    12181 agcgcggcaa tggcgaggcg ctggcgttcg ccgccggaga gggtcagcac cgggcgggcg
    12241 gcgtactccg cgagccccac ggtggccagc gcggcgaggg cgatggcttc gtcgtccggc
    12301 ccttcccagg cccagcggcc gaggtgggga tggcgcccga cgagggccgt ctccagcacc
    12361 gtggcgctga agaaatccgg ctggctctgg ggcagcaggc cgcgcaggca ggcctcgtgg
    12421 ccccgcggcc agtcggcgta gggtttgccg gagagcagca ccgtgccccc cgccgcggga
    12481 cgcaggccgg ccagggtgtg cagcagggtg gtcttaccgg caccgttgcg gccgaggatc
    12541 accagggtat cgccgggcgc gacgcgcacc tccagaccgc gcaccacgtc gcgccggccc
    12601 acctgcacgg cgagggcctc ggcggccagc acgtcggggc gcacgtccat cttcatcggg
    12661 gatgacgcgc cagcaggaag aggaagacgg gcacgccgat cagcgcggtg agcacgccca
    12721 cgggcagctg caccggcgcc accacggtcc gcgcggcggt gtccgccagg gtgagcagga
    12781 cgccgcctgc caggctcgcg gcgggcagca gcaggcgctg gtcattgcca atgaccaggc
    12841 gcaccagatg gggcacgatc aggccgacga agcccaccga gcccagggtg gtcaccgcca
    12901 cgccggtgag cagggcgccg agcccgtaga ccagggcacg cagacgcccg acccgtacgc
    12961 cgagggcgcg ggcggtgact tcgccacggg ccagcaggtt gagctcccgg gcgaagggca
    13021 gcgcggtcag caagcccacg gccagcacgc cgagggccgg ccacgggttc gagacgccgc
    13081 tggcgtcgcc catcagccag aacagcatgc ccttgacgcg accttccggc gcgatgctga
    13141 gcatcagggc caccgccgcg ccgcagccgg cggcgacgat gacgccggtg aggagcagcc
    13201 gtgtgcgcgt ccacgaaccg tctccgcggg ccagaccgaa taccagcagg gtggcgacga
    13261 gggcgccggc gaaggcaccg acgtcgatcc acagccccgc gacgcccgcc agcaggcagc
    13321 ccagggcgcc caccgcggcg ccgccggaga cgcccagcac gtaaggatcg gccaggggat
    13381 tgcgcagcag cacctgcatc agcgcaccgg acagggcgag caggccgccg caggcgaagg
    13441 ccgccacggc gcggggcagg cgcagggtgc ggatgatgtc ggcctccatg ctgcccgcgc
    13501 caccgccgag ggccgccagc aggtccgcag ccgtcaccgg cagctccccc gccgacaggg
    13561 cccaggcgaa ggctgccagc gccgcagcga cgaggaccag cagcaccagg gccgcgcgtt
    13621 gtcgcacgcg ccccatgccg ccagctgtcg tgcgacccgc cggaaccgcc tgcgccatgg
    13681 cccctccgtt catcgtgcct tacctggggg cgtagcgtac gccgaagaac aggttcgcgc
    13741 ccgccgtgtt gtagccccag gcggtctcgt actgcttgtt gagcaggttg ttggcgcggg
    13801 cctccaggct ccagtcccga tccaccgcgt acttcacgaa ggcgttgacc agcccgtagc
    13861 cgcccatgcg gttgatctcg ccggtccggt cgtagcggcc ggccgcgccc tgccattcgg
    13921 cgcccacgtt gaaggcggcg atgcggcggc tgaggtacag gttgccagtc ttgtcggcac
    13981 gccgctgcag gcgcttgccg gtgtcggcgt cctccggatc gaggaagtcg aggttggcgt
    14041 tgatgtcaaa gccggcgagt gtcccggcat agcccaggct caccccctgc aggcgcgcgt
    14101 tattgacgtt ctgggggatg aaattggcgt cgtaggcgat gagattggtg atgcggttgt
    14161 ggaaggcggt caggctcacg ctctgcccgt gctgttccca cagcagaccc agctccttgt
    14221 tataggcgtt ctccggcttc aggttgggat tgccgaagcc cgggaagtag agctcgttga
    14281 aggtcggcgc acggaaggcg gaaccgacgc tgccctgcac gcgcaggctg ggcaggatct
    14341 ggtagccgta gccagcgtag ccggtggtct tgttaccgaa ctgggagttg tcgtcatacc
    14401 gcgcgttgaa ctgcatgcgg tgctcgccca cgctggcggt ccagcccagc agggccgaac
    14461 ggatctggcg actgaccgag aaggtgcctt cgttgacggc cttctgctcc aggttttcca
    14521 gcgccgccag cagcgtgccc aggggcaggg tgatgtcgtt ctgccacatc acctggtcct
    14581 gggtggtctt gatggtggcc ccggtgggcg cgtaggaggc gtaggtgcgg gcgtcatcga
    14641 tcgactggcc gatgcgcagc gtgctcgtcc agcccgcgct gaagcgatta cggctgtagg
    14701 cactccagac gctctgcacg ttgtcggcga agtcgtcgta gccggcgccg gagtcgtagt
    14761 ggttgcgggc cgtggcgtag agcagggtgg cgccgagctc gtggccgggc atcagcgtgt
    14821 aggccagatt gccggagagg ctgctgttgc ggtacggatc cttgtcggcg ttgtagtagt
    14881 aaggctgctt ggcctggctg gcgatggcgt tatagccggc cacgtcggtg taggcgccgc
    14941 gcagggaata ggacaggcgc tcggtcccac cggagacacc tgcggcgact tccttgttgc
    15001 cgtagctgcc gacgccgacg aaggcgtcgg ccttgggggc accctcaccg cggcgggtga
    15061 agatctggat cacgccgccg atggcgtccg caccgtacag ggcggacgct gggccgcgga
    15121 caatctcgat gcgctcgatc tgggtcaggg ggatgtcctg ccaggacgat gcgccgaggg
    15181 tcgcggaacc caggcgcacg ccgtccacga tcagcaggct gtgcttggga ctggcgccgc
    15241 ggatgtacac gtcggtcgcc ttgccggggc cgccgctgct gctcatctgg atgcccggct
    15301 ggcgggccag cagctcggcc aggctggtgc tggcgccggc ctcttcgatg cgggcgcgtt
    15361 cgatgacggt gacgtcggcc agggattcgt cggcgcgctg ggtctggcgc gtggccgtca
    15421 ccagcacgac gggctgctcg gcgctgctca ccgcggcggc agccggggca ttggaggcgg
    15481 cgaaggccgc agacgaaaag acggtcggaa aggcagcagc caccgcaagg gcagccaggg
    15541 tcgggcggat ggtcatggtc ggattctccc aggcccgatg gcgtccccgc cgccgggcgt
    15601 tcgttgtgat gcgcgatcgg ggagaagatc caggcggccc gcgggccacg ccgtcaccgc
    15661 caccccgcgg cacgctcgtg tgtagggttc cgaggccggt ctccgggctg gcaggcggtc
    15721 ggccggaaat ccggaccgga cccggtgcgt cgccttccca gacccgaagg cccagtggcg
    15781 gagtgacgca ccgttcatct gcttaccgtt gcgggggcag cacaggcttt gtggcgtccg
    15841 cagacgtcca cgcacctgtt tcccgtttaa ctccctggcc gggtggccgc aggagcacct
    15901 ggaacacgct cgtcgcctgc ggagacagcc ccgcgggtcg acgagcctgc gataatacca
    15961 cggaatcagc cacttcccgc agcgcaccgc tgcgcccctt cctgccatga gccccgaact
    16021 gatcctcggc ggcgcccgtt cgggcaagag ccgccacgcc gaacacctcg ccgctgcgtc
    16081 cggcctgccg gtcaccgtga tcgtcaccgc cgaggccctg gacgccgaga tggccgcccg
    16141 catcgcccgc caccgcaccg accgcccggc cggctgggcc accgtcgaag ccccccgtga
    16201 cctcgccggg gccctcgttg ccaccgccgc ccacggccgc tgcacggtgg tggactgcct
    16261 caccctgtgg ctcgccaatc tcctcgacgg cgcagagcac ctgccgcccg ccacgccggc
    16321 cgaggcgctg ccggccttcg cccgcgaacg cgccgccttc ctcgacgcgc tgcccaccct
    16381 gcccggccgg gtaatcctgg tggccaacga ggtgggcctc ggcctggtgc cggagacgcc
    16441 cctcgggcgc ctcttccgcg acgaggccgg gcgcctcaat caggccgtgg cggccctgtg
    16501 cgggcgcgtc accttcgttg cggccggcct gccgctggtc ctcaagggcg gctgattgac
    16561 gctacgtcag gcagacgcgg cggcaccggc gctgctagca tccgcagcct cgcctctccg
    16621 gtcgccatca tgcactacac cctcatccgc cccgaccggg gcctcgcccc tgcgctgcag
    16681 cagcgcatcg acaccaagac caagcccctc ggcgcccttg gccgcctgga ggccctggcc
    16741 ctgcagatcg gcctgatcca gcagaccctc gaaccggagc tgcgccagcc ccacgtgatg
    16801 gtcttcgccg gtgaccacgg cgccgcccgc gccggcgtgt cggcctatcc ccaggacgtg
    16861 acctggcaga tggtggagaa cttcctcgcc ggcggcgcgg ccatcaacgt cttcgcgcgc
    16921 caggccggca tgggcctgtc ggtggtggac gcgggcgtgg cccacgactt cggtccgcgc
    16981 cccggcctga tcgccgccaa ggtggcgccc ggcaccgcca actatctgga ggcgccggcc
    17041 atggacgccg cccagcgcaa cgccgccctg gcccacggcc gcgagctggc cctgggcctc
    17101 gccggcaccg gctgcaatgt ggtgggcttc ggcgagatgg gcattggcaa caccgccgcc
    17161 gcgtccctgc tcacccactg cctcaccggc gccgatctgg ccaccgtgat cggccgcggc
    17221 accggcctcg acgacgccgg cctcgcccgc aagcgcgaac tcctggccca cgccgtggcc
    17281 cgcggcggcc gccccgccga tccactggac gccctcgccc agtacggcgg cttcgagatc
    17341 gccatgatgg cgggcgccat gctcggcgcg gcggaggccc gcatggtgct gctcatcgac
    17401 ggcttcatcg tcaccagcgc cctgctggtg gcccacgccc tcgccccggc ggtgctggac
    17461 tactgtgtct tcgcccaccg ctcccaggag cccggccacg ccgtacagtt cgaccatctg
    17521 ggcgccgagc ccctggtgca gctcgatctg cgcctgggcg aaggcaccgg cgcggcgctg
    17581 gcctatcccc tggtccaggc ggccgtgaat ttcctcaacg agatggccag cttcgagtcc
    17641 gccggcgtca gcggcaaggc cgactgagcc ccgtgcgcac ccagctcgaa ctcttcctca
    17701 ccgcggtcgg cttcttcacc cgcctgccgg tgcccggctg ggtgcccttt tcggtcgagc
    17761 gcctggccca tgcggcccgc tacctgcccg ccgtgggctg gctggtgggc ggcctcggcg
    17821 ccctcgctta cgggctggcc agccaggtgc tgccgcccga cgtggccgtc gtgctgtcca
    17881 tggtcgcgac cctcgtcgcc accggcgcct tccacgagga cgggctggcc gacacctgcg
    17941 acggcctcgg cgggggcctc gaccgggacc gcgccctggc catcatgaag gactcgcgca
    18001 tcggcagcta cggcgccatc gggctggtcc tggccctgct gctcaaggcc ctggccctgg
    18061 tcaatctggg cgtcgacgcg gacatgccgg tgatcctagc cctcctcgct gcccaccccc
    18121 tgtcgcgcct cgcgccggtg acgctgatgg ccgtgctcga ctacgcccgc acaggtcacg
    18181 acgccaaagc cggcgccgtg gcccgtcgcc tcacgccggc ggagctgggc attgccaccc
    18241 tcgccggcgt gctgcccctg ctgctcctcg acgcgaccga agccggcctg gccctggccg
    18301 ccaccgtcat cgtcacggcg tgggcgggcc gcctcttccg ccgccgcctc gacggctaca
    18361 ccggcgattg tctgggcgcc acccagcaga tcgccgagct cgccatctac ctcgccctgc
    18421 tcgccgcgtg gaacttcacc tgatccgcca cccacgcccg gacgtggcgc ccggtgtgtg
    18481 ttacgggcag accgacgtgg gcctcgccga atccgccgcg gcggtcgccg cccgcctgcg
    18541 cccgctgctg ccggcgggct gcgccctgat tgccagccca ctgcgacgcg cacgcctgct
    18601 cgccgaagcc ctcggcacgc cgcagctcga cccgcgcctg atggaaatcc atttcggcga
    18661 ctgggaaggg cagcgctacg ccgacctggg cagcgccctc gacgcctggg ccgccgcccc
    18721 cctggatttc gccccgcccg gcggcgagac gccgcgccag atggccgcgc gggtgctcga
    18781 cttcctccac gacctgcagg cgacaccgcc cgccgccgcg gtcgccatcg tcgcccacgg
    18841 cggcccgctg cgggtcattg ccggccacct gctgggactg cccgacacgc actggctcgg
    18901 gctcgacttc gcctgcggcg aagcaacccg catcgactgc cacgactggg gcaccgtcct
    18961 gcgctggttc aaccggcgcg cctgacgccc tgccatccgg gggatcctgt cctcccgaca
    19021 ggatccccac cggggtacaa tccgatttgc atgcccgaat gtcatgcacc cgcacagagg
    19081 ttccgccctc gagccgtcca ccgaagatcg ccagggccgt ctgccaacgt ctgctcagga
    19141 tcgccatgaa ccgccccttc cgcccggccc tgctggccgc cgccatcgct gccgccgtgc
    19201 ccgcccacgc ctatgtcgcc accgcctttg acgccagcgt ctataccgcc aacaccgcgg
    19261 cgctcgacag cgctctcggc ctgaccggca tcgtcgccat cgaggacttc gaggacggcg
    19321 tcctcatccc tggcctgacc tccaacctca ccacctacgg cgcgtccctg cccaatcacg
    19381 cctgggacgg cctcaccgcc agcacctggg ggaatgcgct gttcaccgtg agcctgacgg
    19441 gcatccgcca gttcggcatc ggcatctccg acaacgacgg cggcggccag accgtcaccg
    19501 tcaatgggca cttcaccctc gatctgtcca cggtggccgg ctatcggggc gacacctcgg
    19561 gccgcgccac ctatgtgctg atccacgccg agtcgggaga cgccgacatc acgcgtatcg
    19621 ctatcgacaa cagcctgaca ccgctgttcg accacctggt gctcagcacc gactccggcc
    19681 tcgcgccgtc cgccctgccc gagccctcca gtgccgcgct gatgctcgcc gggaccgcac
    19741 tcgcaggctg gctgagggga cgtaaccgca tgaagtaaag ttgtttttta ttcagtaaaa
    19801 agacgaccgc attggttaaa cttcttcaac tgcgggcccg cggtcgccag aaccggggag
    19861 tgggatgcta gggtatcgag gcttccccaa cgccatgccc gtccatgaac agcattcgat
    19921 cggggcccat ccgcaggccc cccaagccca tcacctggtg gcacgccctg gccggcgtgc
    19981 tcgtcgggtc cctcctcgcc acccttctcg cgtggatgtt gccgcgcacc gacgcggagg
    20041 agctgctgct gccggtgcat gccacctacg ccgcccgccc agcccgtggg gacggcctgc
    20101 ctgccggcct cagcctgcgc ggcatcatcc ccccctccgg cgacagcccg ggcattgctc
    20161 tgctcaagga gcccggccgg ccggtcgtgc tggtcaccga ggggcatgaa tacgccccgg
    20221 acctgcgcgt cgtgcgggtg ctccaggacc gcatcctgat tcgccagcgc ggcaatggcg
    20281 acgacctcat cctgaccctg gtcgacccgt catccgggag tcccgtgccc tgagcgccaa
    20341 agcgtcggat aaactgacaa cgcgccgcac gcgccccggc ccgcattttt caaggccttg
    20401 aactcaaagg aattgttaaa attggcactc ctgttgcttt aggtaaattg cctgaatttt
    20461 gcaatttaca tgggggtttc agccatgatg ttccgcgcgt ctttccagca tgccctgtcc
    20521 gccgtgctgg tcaccgccgc cctgacgggc accgcgactg ccgcgacggt caccccgtcg
    20581 tcctatacct acgaccagtc caccgcctgc ggcacctggt gttatcacga ccccgccaac
    20641 accaagctca ccgacggcgt cctgggcctc gccggctggg ccgtcaatgc cggcaacgaa
    20701 tgggatggct gggtctatca gaaccgggtc aatatcgatt tcgactttgg cacctcgcgc
    20761 cacatcgatc tggtgcgcgt cggcacgacg caggacaacc tgggcgacgt ggtgctgccg
    20821 tccatcgagc tgtacatcag caatgacaag tccacctgga ccttcgtcac cggcctcacc
    20881 gtcccgcccg acagcgctca caacatcgac cccttcagca ccgcccctca cgacttcctg
    20941 accctggccg gtctgggcat cgacgcccgc tacgtgcgtg tcggcgccat cgccaacggc
    21001 ccgtggatct tcgtggacga ggtccagttt cagagccgca ccacccagcc ggtcccagag
    21061 cccgccgcgc tgggcctcct cgccgccggt ctcgccagtc tggcgggtac ccgccggcca
    21121 cgctgaaccc gcgcctgcgc ccggcgccgg atgtgccgcc gcctcagtcg gcggatggca
    21181 catccggcag gcgcttcgcg gccgtccgcg tcccccgccg cgtccccgcg gggcgcccct
    21241 tccggccgag ccggtggcgg atctcgtccg aatccatcca caggtcgctg cccttcagga
    21301 tgcggtcgat gtccgccgcg ctcaggaagg gcgagcagat ccgcgtcgtg accttctcga
    21361 accacgcgcc cagggcctgc acctcggcca gctgctcgtt gcccttgcct gcaaagctgc
    21421 cggaataggt gtggaacatg agctggcagt tgtcgtggac gatgagctcg tcgccggaca
    21481 ggaagacgaa ggccgccatt gaataggcac gcgcctccag caccgtcacc acgcgggcct
    21541 cggaggcgag catgttgttg atgatctgca ggccggtgtt gaaatcccca cccggcgagt
    21601 tgatgtgcag atacaccaag tctgtcgccg acgccgtgcg cagggtgtag aacagctcgg
    21661 tgtagtactg gggctcacgg atctcaccgc acaggtagta ggacacctgg tgaatcggca
    21721 cgtggcgctc gtagcggatc aacccgctga agggcaccag gccctcttcg tccatctcct
    21781 cttcccgacc cggcgcgcgt ggcatgg
//
LOCUS       DAOQMM010000058        21597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_834_length_21597_cov_46.115960, whole genome shotgun sequence.
ACCESSION   DAOQMM010000058 DAOQMM010000000
VERSION     DAOQMM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21597
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_834_length_21597_cov_46.115960"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..597)
                     /locus_tag="PLH85_10510"
     CDS             complement(25..597)
                     /locus_tag="PLH85_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNB65041.1"
                     /translation="MTSNLVGLLLAAGRGTRFGSDKLLHPLPDGTPLAVAAARTLQAA
                     CPRCVAVLRPEQTGLAECLTREGLAVVFSPEARAGMGHSLAAGVATTPEAAGWLVTLA
                     DMPFLRVDTVQAVMQALAGGAALAAPVCAGRRGHPVGFAARWRDELQGLTGDEGARAL
                     LGRHRALIVGIETDDAGVLRDVDVREDLGG"
     gene            complement(612..1613)
                     /locus_tag="PLH85_10515"
     CDS             complement(612..1613)
                     /locus_tag="PLH85_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNB65042.1"
                     /translation="MDGQDLQVLAAVRRWLADGHRVALVTVARTWGSAPRPPGAWMAL
                     RDDGRVQGSVSGGCIEDDLIARMGAGEIVGRAPFVLRYGVTRDEAARFGLPCGGTLEL
                     VVEPAPDRALLATLAERLTAGRLVRRDVDLASGATRLADAHRGEAMAWDGTRLSTLHG
                     PRLRLLLIGAGQIARSLAPMAQALDYAVSVCDPREEYAREWDVPGVPWVAGMPDDAVL
                     AMNPDPHSAIVALTHDPKLDDMALLEALKSPAFYVGALGSRSNNAARKARLLRYFDLS
                     DAEVARLHGPVGIPIGSRTPPEIAVSILAGLTAVKNGVALPLAVTDAPPASAGCGVA"
     gene            complement(1730..3934)
                     /locus_tag="PLH85_10520"
     CDS             complement(1730..3934)
                     /locus_tag="PLH85_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNB65043.1"
                     /translation="MSHSIHNVSRRQFLQGAAGLSLGLALPAFGARIEAGPGKAGMMV
                     GEAAFEPNAFLRIGVDNSVTVISKHLEMGQGTYTGLATLVAEELDAAWSQVKVEGAPA
                     DAKRYNNLFWGPAQGTGGSTAMANSWEQMRKAGAAARAMLVNAAAKRWSVPAGEITVR
                     DGLIQHAPSRRSAGFGEMAVAAGAEAVPASVTLKDPRDFRLIGRQAPRKDSAAKTNGT
                     AQFTQDLHLPDMLVAVVAHPPRFGATVKSVDAKAARAVKGVVDVVTIPTGVAVLARTT
                     WSARKGRDALKIDWDESAAFRLGSDEILARYRDLARTPGLVARKEGDVGAALAGARKV
                     ITADYDFPYLAHAAMEPMNCVVQRTAAGVEVWNGEQLHTGDQHALAALFGVAVDDVNI
                     HMLYAGGSFGRRACKDSDYVREAASIVKAAGTQAPVKLVWFREDDMRAGYYRPLFHHR
                     LEAAVDEGGRIVAWRHRLVGQSIIKGSPFESFLIKDGIDAVSVEGAATLPYAIPHLQV
                     ELHTPSDINVPVLWWRSVGSTHTAYSTETFLDRLAASAGQDPVALRLHLLEKHPRHAA
                     VLRLAADKAGWGTPLAAGKPGERRGRGVAVHESFHSFVAQVAEVTVAADGSVKVDRIV
                     AAVDCGVAINPDNVRAQVEGSVAFALSAALHGEITLQEGRVVQSNFHDYAPLRLNEMP
                     RVEVHIVPSAANPTGIGEPGVPPVAPAVANAIAAATGTWLTRLPFNADALKA"
     gene            complement(3945..4403)
                     /locus_tag="PLH85_10525"
     CDS             complement(3945..4403)
                     /locus_tag="PLH85_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNB65044.1"
                     /translation="MTPLTVNGVRHRVDVEPDTPLLWALRDALLLTGTKYGCGAGLCG
                     ACTVHVDGQPVRSCSTPVSAAAGKKITTIEAADQSRVGKAVQAAWRKLDVVQCGYCQS
                     GQIMSAIALLNTHRAPSDADIDAALAGNLCRCGTYVRIRAAIHEAAKALA"
     gene            4625..5542
                     /locus_tag="PLH85_10530"
     CDS             4625..5542
                     /locus_tag="PLH85_10530"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB65045.1"
                     /translation="MYGAGLSPALPLHAADRRTSPLAALIERYCPDDGHRETQVPGLD
                     LFRASDAGSPVCGVYRPVLAVIAQGCKRIGLGDETYVYDSQHCLVSAVDLPVTAQIVG
                     ASRERPYLCAVIELDPRRIADVALAMDLPRPAPAAPRGLGVSPLTPAVADAVLRLVQL
                     LETPEDIPFLAPLIERELLYRLLAGEQGLRLRALATADSQSQRTARAMEWIRHHYHLP
                     LSIDALAETANMSKSSLHHHFKALTAMSPLQYQKQIRLQEARRILLTEDADAATVARR
                     VGYESPSQFSREYRRQFGAPPLRDIARLR"
     gene            5677..6390
                     /locus_tag="PLH85_10535"
     CDS             5677..6390
                     /locus_tag="PLH85_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB65046.1"
                     /translation="MTEDLHARPAVVPPGFFDPPDEDDAPAHASDGDDAPLAVRPGPL
                     VDADPQRLRELLARVADEDEDALAALYEATGSRVYGLALRILRDPAAAEEVTEDAYFQ
                     VWRQARRYDPARGRPLAWLLTIVRSRALDHLRRDDPALPHPEPVSLLDAEPEHTDNPQ
                     NLLEACRDHDRLHAALAGLDPVPRQLIALVFFRGLTHEEAADHARMPLGTVKSHVRRA
                     LLALRAVLAADLDRSYSGS"
     gene            6387..7211
                     /locus_tag="PLH85_10540"
     CDS             6387..7211
                     /locus_tag="PLH85_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB65047.1"
                     /translation="MKPTYPLDPDQPDASADVEARLLESLTPLPLPEARGRALASRLQ
                     ERIRASRDAGRGLITLRRDQGHWAPLVPGVRVKRLSGGQRAVLLDLAPGAVIPFHRHH
                     EDEECVVLRGSAQLGDVTVHPGDYHLATTGSRHGVVRSETGALLFLGGTPIGHGLEVA
                     RDVVTALLPGRGQAPLTVPRDEGPWTDPAPGLHLKLLRDDGHGRSLLVRLEAGCRADL
                     AELAGHGECLMVEGEAFAGDTLLQAGDYQLAPQGVTRPTLSSDVGGLLFVRTGLRV"
     gene            complement(7201..8391)
                     /locus_tag="PLH85_10545"
     CDS             complement(7201..8391)
                     /locus_tag="PLH85_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNB65048.1"
                     /translation="MLDAVEEIRAFNAGRDPERLVLKFRNMRSDPFVFLRATCHLFYR
                     RLPELPDSPAVWCCGDLHLQNFGSYKGDNRLVYFDLNDFDEGALAPAHWELVRFLTSV
                     HAGAPSLGVKRKEAAALCGDFLDAYRERLVAGKALWVERETASGLVHQLLDGLRKRER
                     AAFLDGRTVRKGKKRLIRLDNGKALPVTEKQRERIAQFMAGVARTRDDPAFYEVLDVA
                     RRIAGNGSLGVDRFVILVRGKGSPDGHYLLDLKEALPSALLPVLKTPQPAWKSEAARV
                     VAVQRRMQAVSMAFLLPERLGKRAYLLRELQPTEDRVTLDAARSGLPALREAVATMGH
                     IVASAQLRSSGRDGSAIADQLIEFGAARWRKSLLAAAEASAKQLRADWKTFCAAYDDG
                     VFTP"
     gene            complement(8439..8897)
                     /locus_tag="PLH85_10550"
     CDS             complement(8439..8897)
                     /locus_tag="PLH85_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65049.1"
                     /translation="MKRLLCLLLAASGAAMAAPADPLTAVAEGAVYAQSRCYRLIHHD
                     TFDYEDCLTALLKDERRPTPRRLGMEYFGWVGAQNSARLGMRGAEQSAYEFLGRFRAT
                     QKRLRIGDEALCATVAGDCTVRIARMKQMEAAPRPRPAPGEGDDDERPHH"
     gene            complement(8965..10779)
                     /locus_tag="PLH85_10555"
     CDS             complement(8965..10779)
                     /locus_tag="PLH85_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNB65050.1"
                     /translation="MKRPVSTKMAAWLAALSTAWAVTGCGGGASSSESAAPVSVKLIA
                     MNDFHGNIEVPAANNGGSVVLKDPANAAGTTVRSGGAAYLATLIKQLKAQNPYSIVVG
                     AGDMVGASPVTSTLTHDEATVDILNQIGLEVTSVGNHEFDHGKTELLRLQNGGCYPGG
                     TIGQDTCIKGGSFSGATYKWLSANVVDSSSGQSLFPATYVKKFGPVSVGFIGLTLKGT
                     PQVVTSTGVAGLDFQDEATTINKYAASLKASGVNAVVVLIHQGGQTTASTLNDKTCPN
                     LTGDILPIVDALSSNVDVVVSGHTHQEYVCTRNGKLLTSTGFYGSAVTEINLTLLPGG
                     GVLTRDANTRPVINDLNTTVPAGFSILTKDATIDAAVQSYVSLSATLKNQVVGSITAD
                     IKRALLSSSSTPTRDETAEGAMGDVMADVYLAGGPKADIAFINPGGVRADLIYKNGGA
                     VTYGDLLTVAPFGNTLVTVDLTGADLVRLLEQQWEAPNCAAKTGANGCGRMLQPSSTF
                     SYTWDASKPAGAAVGSGNRVVAGSLKLNGVAMDLTKTYRVTLNSFMAPGPGDNFSVVT
                     ANGKNVTPSGVIDIDAFVGYMKAHPSLAPPAARITRLN"
     gene            complement(10853..12874)
                     /locus_tag="PLH85_10560"
     CDS             complement(10853..12874)
                     /locus_tag="PLH85_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNB65051.1"
                     /translation="MSAALPPEVRVAVPSRLLRGRCRPVVALVGLPRTGKSTLFDAAS
                     STAVQSGQLDGSALGYRACVVNVGLEQAGVVDLPPLRTLLDLDEADRRVLTALLEGEP
                     GEGGFRAPDVLVHVVDATALTRDLALSQELCLLGKPLVIALNRLDEAREKGIYINTAA
                     LAEALGVTVVPTVAHMGKGISALFEAALEAVRRSVCPVPQPASPHLRQALHGFDAVLD
                     LPEVEAAFPVPRALLLTQLAKADTLLAREFAERFPALMPRIAAARKAAGAQLPRSLSE
                     ELFADRHYRAALLHEQVTRLGHAAEKAGWKRQLDRLFLHPRWGLIGTLAVFALVLYVV
                     FEVSTGLDSVTSAPLAAWVGEWQPDTTAGVIGRAIADGLVGLVGIVVPYMLPLVLLLV
                     GLEESGIMHRVAFVVDRGFHRIGLHGGVAVPFLLGLGCNVPALSAVAASSAGRERIVA
                     SLLITFVPCSARSAIVLAIGGKYLGWEGVLGIFLLTMLVIAVGGRLLTRRYVQASPGM
                     IQAIPAYALPRWRKVLAVTWERTSDILTIVTPLLVLGSVVLALLTHFGADEVINTVLV
                     PLTSWSLGLPVVLGVPILFGVLRKELSLLMVYQALGTQEIAPLLDWVQIATFLVFITF
                     YVPCVSTFAVMLKTVGRREAVFSLGLSVAVALGIAVACRILLHGVNLLL"
     gene            complement(13063..13710)
                     /locus_tag="PLH85_10565"
     CDS             complement(13063..13710)
                     /locus_tag="PLH85_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB65052.1"
                     /translation="MMPTRILIIEDHVLVREAMALTLAQVGEGVFCQQASSAAEAIAL
                     LEEDPDCDLLVVDLMLPEMNGFSLLGVIAKRFPDVPALVVSALDDRESVHRAMKAGAS
                     GFVSKASPSNTLIEAVRTVLDGGVFTPEATSGSDGSGRTRRASQAFAERFQLTAAQGR
                     VLELVAQGKSNRDIADLLGLSEGTVKVHVSAILRSLGVTSRAQALLLMTRAGLRV"
     gene            13830..15893
                     /locus_tag="PLH85_10570"
     CDS             13830..15893
                     /locus_tag="PLH85_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB65053.1"
                     /translation="MPRIAFAPFNAAGLLTSLFSLLTPSAAGAAPNGEDSFLDDLPVV
                     LSATRLTQAPADAPGSVTVLDRDMIRASGARNISELFRLVPGFQVAMQTGNTPQVTYH
                     GLSDEAPRRMLVQVDGRSVYSAYFISGVEWNQIGVDLDDIERIEVFRGSNSAAYGSNA
                     FLGVANIITRPAAETQGVALRLRGGSDGVNDQGVRIGQRLGDVDLRVTAARSHDNGFA
                     DLNDWRRNELANLRADWHIQHDHQVEFQAGWTRNEFGTGKANNLSDPERSAELSTGFG
                     LLRWRHTPQPDEELSVTYYHQQETGKDAYPYTVIVPPHLTGLPVNLQIPFFFEYGYNV
                     TRDDLEAQRITSLSPTLRAVYGGGLRSDRSHAPLRFNTADTVRSDTRHLFGTLEWRAS
                     PAWLFNLGAMLENSSLTGTSVDPRISANYHFSDISTWRASINRSHRHPSAFEQKANMV
                     FRNSAAFPTPIGAVPAGTVISQTFRPSPDITAERITTYEIGYLAELRSLATTIDARAF
                     VERARNLIEMNIENSTIGLVPRDNVRYFANTGEADIRGVELSGTWRPAAQTWVNLTHT
                     ELRIAGPTVTAAQAARQSTPYVSNTAPKHTSTVFAAWEFVPRWQVSASKRWVGSMSWY
                     QDDPHAIPMYRQLDVRIAHRFQTAYGRGEVALVGQNIDGGEQTFTSTAGVWGSRTFAT
                     LSLEL"
     gene            15904..16824
                     /locus_tag="PLH85_10575"
     CDS             15904..16824
                     /locus_tag="PLH85_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNB65054.1"
                     /translation="MGCTIRRFLLILLTLAGLASPARAALVALVSADEGAAFSEAADA
                     ITAELRGAGHRVQSFTLPLRADDTGFASAALIVTLGSRAAQLVSTLPQHGPVLHTLLP
                     RSAFDRLPRPDDPRRVSAVYIDQPAGRQLELIRQALPDWQRLAVITGRDSAELGTRLQ
                     AAAREHRSLRVQTDIALGERDIYPVLQRMLAEPAILVAVPDATLFNSNTISNILLTAY
                     HHRSPVVGFSAAYIKAGALLALYSTPAQIGQQAGEVARQALASGTLPPPQYPRQFRVG
                     TNAYVARSLGITLEDPNALRDRLERTEGTP"
     gene            16821..18716
                     /locus_tag="PLH85_10580"
     CDS             16821..18716
                     /locus_tag="PLH85_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65055.1"
                     /translation="MSARDLDIGTRALVIAVLPAFVVAVVLIAYFTHSRLNDLEDAHS
                     QRGKALARQLAAASSYVVFTGNKDALGKLAAEILDEPDVVRVAVRGPGKDTLTDLRKP
                     APSDSGALRLGNADSDTLAFQEAIHSPGQLFDDPFVGAEGAGSELHAQQGEVIVELSR
                     DALHGEETRLLRNAVLMVFAVLLGSVVLALRLSRGITRPIQRIASTVARIGQGERAAR
                     VRVEGGHTLRALGQGVNDMADRIAAASENLERQIEAATAELLEKKNEAELANQAKTRF
                     LAAASHDLRQPMHALGLFVAELGQKPHAPDTCTLVEQIAMSAETMEDLLDSLLDISRL
                     DAGALEPQIKPFPLQPLLERVDVDYRREAERRGQRLRLRATDLWVSSDPLLLERILHN
                     LISNALRYAPGGTVLVACRRRGAQVTVEVRDNGPGIAPDAQEAIFHEFVQLDNPERNR
                     AKGLGLGLAIVRRLTTLLGHPLGLRSRPGRGALFSVTLPVTAPRPQDLSPPSSAARGL
                     AGLHVALIDDDPLAAASTLSLLESWGCLVTAADTLEHMYAALQRSEMSPDIMLCDHHL
                     DGALDGVAVVARLRQLLGTDLTAIILSSDTTLADSPRVRDNGIRVLRKPVRPARLRAL
                     LQRKTGS"
     gene            18785..19204
                     /locus_tag="PLH85_10585"
     CDS             18785..19204
                     /locus_tag="PLH85_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65056.1"
                     /translation="MSADNTMQDPMEFMRNMWSKMGFSLPGMVTPTLDLEELDRRIND
                     MKTVENWLKMNLSSLQMATQGLEMQRATLAAMQAMSKVATEGAGKVDEGTPNPFASTM
                     WPWNFVPAAAEAAKAAAAPAESAEPAAKPAAKSKSAK"
     gene            complement(19249..20157)
                     /locus_tag="PLH85_10590"
     CDS             complement(19249..20157)
                     /locus_tag="PLH85_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB65057.1"
                     /translation="MQSFLLLLPDFALIALGVVLRRFGRLEDGFWAGVEKLVYFVFFP
                     ALLFHALARAPIDLSAAAPLFAVGLLTMASGFALALLGRPLMGLGEMAFASRVQCAYR
                     FNTYIGIAVAGTLHGAAGVAAMGALCGAMVPFANMAAVGFLAHHGQGRLLRELARNPL
                     VIATLAGLAFSTAGLHLPGPVLPFLQRLADAAVTLGLIAVGAALRIGQREGRPVGMVY
                     LCAVKLLALPAVAWGLAGAFGLEGLAFATAVLFAALPTASSAYILAMRMGGDGPGVAW
                     LISATTVAAAVTLTGWLTALRGVAAG"
     gene            complement(20349..21584)
                     /locus_tag="PLH85_10595"
     CDS             complement(20349..21584)
                     /locus_tag="PLH85_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB65058.1"
                     /translation="MNYYDARETRDPAERERDLLARLPVQVAHAQARTPAFARLLAEV
                     DAAAVTSREALASLPVIRKSELSALQKAARPFGGLAALGWGAGCARVFASPGPIYEPE
                     GARKDYWRLARAFYAAGFRAGDLVHNTFSYHFTPAGSMMETAAHALGCTVFPAGVGQT
                     EQQLAAMLDLRPDAYAGTPSFLRILLDKAAEQGLRLDSLKKAFVSGEAFPPSQRDALA
                     AAGIQAFQAYATADLGLVAYETPAREGMVVDEDVIVEIVRPGTGDPVAPGEVGEVVVT
                     TFNPDYPLIRFGTGDLSAILPGASPCGRTNARIRGWLGRADQTTKVKGMFVHPGQVAA
                     VLKRHPEILRGRLVVDNPDLNDRMTLHCEVAGDGAGLAEAVVASLRELTKLRGEVQCC
                     APGSLPNDGKVIDDVRRYA"
BASE COUNT         3451 a         8074 c         7091 g         2981 t
ORIGIN      
        1 ccccccacgg ggcacgaggc cccctcagcc gcccagatcc tcccgcacat ccacatcccg
       61 caacaccccc gcatcgtccg tctcgatgcc gacgatcagc gcccgatgac gtcccagcag
      121 cgcgcgggcg ccttcatcgc ccgtcagccc ctgcagttcg tcgcgccagc gggcggcgaa
      181 gccgacagga tggccccggc gacccgcgca gacgggcgcc gccagcgccg cgccgccggc
      241 cagcgcctgc atcacggcct gcaccgtatc gacccgcagg aagggcatgt cggccagggt
      301 gacgagccag ccggcagcct ccggcgtcgt cgccacgccg gccgcaaggc tgtggcccat
      361 gccggcacgg gcctccgggc tgaacaccac ggccaggcct tcccgcgtca ggcattccgc
      421 cagcccggtc tgctcgggac gcagcaccgc cacgcagcgc gggcaggcgg cctgcaaggt
      481 acgcgcagcg gccaccgcga ggggcgtgcc gtcgggcagc gggtggagca gcttgtcgct
      541 gccgaaacgg gtgccccgcc ccgccgccag caggaggccg acaaggttcg acgtcaccgc
      601 acctgccgac gtcaggctac gccgcagccg gcgctggcgg gcggcgcgtc ggtgacggcc
      661 agcggcaacg ccacgccgtt cttgacggcg gtgaggccgg ccaggatcga caccgcgatc
      721 tccggcggcg tacggctgcc gatgggaatg cccaccggac cgtggaggcg cgccacctcc
      781 gcatccgaga ggtcgaagta acgcagcaga cgcgccttgc gggccgcgtt gttgctccgc
      841 gagccgaggg ctcccacata gaaggccggc gacttgagcg cctccagcag ggccatgtcg
      901 tccagcttgg gatcgtgggt cagggccacg atggcgctgt gggggtcggg attcatcgcc
      961 agcaccgcgt cgtcgggcat gccggccacc cagggcacgc cgggcacatc ccactcgcgg
     1021 gcgtattcct cccgcggatc gcagacgctg acggcgtagt ccagggcctg ggccatgggc
     1081 gccaggctgc gggcgatctg gcccgcgccg atgaggagca ggcgcagacg cggtccgtgc
     1141 agcgtggaca ggcgcgtgcc gtcccaggcc atggcctcgc cccggtgagc atcggccagg
     1201 cgcgtcgcgc cgctggccag atccacgtcg cggcgtacca gccgccctgc cgtcagacgc
     1261 tcagcgaggg tcgccagcag ggccctgtca ggcgccggtt ccaccaccag ttccagcgta
     1321 ccgccgcagg gcaggccgaa acgggccgcc tcatcgcggg tgacgccgta acgcagcacg
     1381 aagggtgcac ggccgacaat ctcgccggcc cccatgcggg cgatcagatc atcttcgatg
     1441 cagccgccgg acaccgaacc ctgtacccgg ccatcgtccc gcagggccat ccaggcgccg
     1501 ggcggacggg gcgccgagcc ccacgtgcgg gcgaccgtga ccagcgcgac gcggtggccg
     1561 tcggcgagcc agcggcgcac ggccgcgagc acctgcagat cctggccgtc catgtcagat
     1621 ctcctgcccc gcgccccgca cggcagtcgg gccccgggcg ggcgcgctac cgtgccgcac
     1681 gctcgccacg gcggacccgg cgtcgtcaag ctgaagtcgg gccatcatcc taggctttca
     1741 gcgcgtcggc attgaagggc agacgggtca gccaggtgcc cgtggccgcg gcgatggcgt
     1801 tggccaccgc gggcgccacg ggcggcacgc cgggctcgcc gatgccggtc ggattggccg
     1861 ccgagggcac gatgtgcacc tcgacccgcg gcatctcgtt gaggcgcagg ggggcgtagt
     1921 catggaagtt ggactgcacc acgcggccct cctgcagggt gatctccccg tgcagggcgg
     1981 cggagagcgc gaaggccacc gaaccttcga cctgggcgcg cacgttgtcc gggttgatcg
     2041 ccacgccgca atccaccgcg gcgacgatcc gatccacctt cacgctgccg tccgccgcga
     2101 cggtgacttc cgccacctgg gcgacgaagg aatggaaaga ctcatgcacc gccacgccac
     2161 ggccgcgccg ctcgccgggc ttgcccgccg ccaggggcgt gccccagccg gccttgtccg
     2221 ccgccagccg cagcaccgcg gcatggcgcg gatgcttctc cagcaggtgc aggcgcaggg
     2281 cgacgggatc ctgaccggcg ctggccgcca gccggtcgag gaaagtctcg gtggagtagg
     2341 ctgtgtgggt cgagcccacc gagcgccacc acagcaccgg cacgttgatg tcgctgggcg
     2401 tgtgcagttc cacctgcagg tgggggatgg cgtagggcag cgtggcggcg ccttccaccg
     2461 acacggcgtc gatgccgtcc ttgatcagga aggactcgaa gggcgagccc ttgatgatgg
     2521 actgcccgac caggcgatga cgccaggcga cgatgcgccc gccctcgtcc acagccgctt
     2581 cgagacggtg gtggaagagc ggccggtagt agcccgcccg catgtcgtcc tcgcggaacc
     2641 acaccagctt gaccggcgcc tgcgtgcccg cggccttgac gatggaagcc gcctccctga
     2701 cgtagtccga atccttgcac gcccgccggc cgaagctgcc gccggcgtag agcatgtgga
     2761 tgttcacgtc atccaccgcc accccgaaca gcgccgccag ggcatgctgg tcacccgtgt
     2821 ggagctgctc gccgttccac acctccacac cagccgcggt gcgctgcacg acgcaattca
     2881 tgggctccat ggcagcgtgg gccaggtagg gaaagtcgta atcggcggtg atcaccttgc
     2941 gggcgccggc cagcgcggcc cccacgtcgc cctccttgcg ggcgaccaga ccgggcgtgc
     3001 gggccagatc ccggtagcgg gcgaggatct cgtcgctgcc cagacggaag gccgcgcttt
     3061 cgtcccagtc gatcttcagc gcgtcgcgcc ccttgcgggc gctccaggtg gtccgggcca
     3121 gcaccgccac gccagtcggg atcgtcacca cgtccaccac gcccttcacg gcgcgggccg
     3181 ccttggcgtc gacggacttc accgtggcgc caaagcgcgg cgggtgcgcc accaccgcca
     3241 cgagcatgtc cggcagatgc aaatcctggg taaattgggc cgttccattg gtcttggcgg
     3301 cactgtcctt gcgcggcgcc tggcggccga tcaggcggaa atccctgggg tccttgaggg
     3361 tgacgctcgc cggcacggcc tcggcgccgg ccgccacggc catctcgccg aaacccgcgc
     3421 tgcgccggct cggcgcgtgc tggatcagcc cgtcccggac ggtgatctcc cccgccggca
     3481 cggaccagcg cttggccgcg gcattcacca gcatggcacg ggcggcggcg ccggccttgc
     3541 gcatctgctc ccaggaattg gccatggcgg tactgccgcc cgtgccctgg gccgggcccc
     3601 agaacaggtt gttatagcgc ttggcgtcgg ccggcgcacc ttccaccttc acctgggacc
     3661 aggctgcgtc cagttcctcg gccaccaggg tggccaggcc cgtataggtg ccctgcccca
     3721 tctccaggtg cttggagatc accgtgacgc tgttgtccac gccgatgcgc aggaaggcgt
     3781 tgggctcgaa ggccgcctcg ccgaccatca tgccggcctt accgggaccg gcttcgatgc
     3841 gggcaccgaa ggcgggcagg gcgaggccca ggctgagacc ggcggcccct tgcaggaact
     3901 ggcggcggga gacgttgtgg atcgaatggc tcatcgcgat ctcctcaggc cagcgccttg
     3961 gcggcttcgt ggatggcggc gcggatgcgc acgtaggtac cgcagcgaca caggttgccg
     4021 gccagggcgg catcgatgtc cgcgtcgctg ggcgcgcggt gggtattcag cagggcgatg
     4081 gcgctcatga tctggccgga ctggcagtag ccgcactgga ccacgtccag cttgcgccac
     4141 gccgcctgca ccgccttgcc cacgcgggac tggtcggccg cctcgatggt ggtgatcttc
     4201 ttgcccgccg cggccgacac cggggtgctg cacgaacgca ccggctggcc gtccacatgc
     4261 acggtacagg cgccgcagag gccggcgccg cagccatact tggtaccggt gagcagcagg
     4321 gcatcgcgca gggcccacag caggggcgta tcgggttcga catccacgcg gtggcggacg
     4381 ccgttgacgg tgagcggggt catggtcgtt tctccgttgg gatcggggcg ggccgtcggc
     4441 cgacaggcgc ccgtgacgag aatcaggggc gacagggacg cggcggatct gcgctgaact
     4501 gtagcccaag gccctgccgg acgcattgca gaaagctgcc gatttattgc ccgatcctcc
     4561 gatcatggcg cagcccacgt ccgggcaggc tacacttcag cggcatcctc agaggacaga
     4621 aaccatgtac ggagcaggcc tttcccccgc cctccccctc catgccgccg accggaggac
     4681 gagcccgctg gcggcgctga ttgaacgata ttgcccggac gatggccatc gcgaaacgca
     4741 ggtgccgggc ctcgacctgt tccgggccag cgacgcggga tcgccggtgt gcggggtgta
     4801 tcgccccgtg ctggcggtga tcgcccaggg ctgcaagcgc atcggcctcg gcgacgagac
     4861 ctatgtgtat gacagccagc actgcctcgt atcggccgtg gatctgcctg tcaccgccca
     4921 gattgtcggc gccagccggg agcgacccta cctgtgcgcc gtgatcgaac tggacccgcg
     4981 gcgcatcgcc gacgtcgccc tcgccatgga cctgccccgc ccggcacccg ccgcaccccg
     5041 cggactcggc gtcagtcccc tcaccccggc ggtggccgac gccgtgctgc gtctcgtgca
     5101 attgctggag acgcccgagg acattccctt cctggccccc ctgatcgaac gggagctgct
     5161 ctaccgtctg ctcgccggcg aacaaggcct gcgcctgcgc gccctggcca ccgccgacag
     5221 ccagagccag cgcaccgccc gggcgatgga atggatccgt caccactacc acctgcccct
     5281 gtccatcgac gccctggcag agacggccaa catgagcaag tcgtccctgc accaccactt
     5341 caaggccctc accgccatga gcccgctgca gtatcagaaa cagatccgcc tgcaggaggc
     5401 gcggcgcatc ctcctcaccg aggacgccga tgccgccacg gtggcgcggc gggtcggcta
     5461 cgagagcccg tcccagttca gccgcgaata ccgtcgccag ttcggtgccc cacccctgcg
     5521 cgacatcgcc cggctgcgct gacgggggct gcatccggat cggccgcgtc tgcgtagacc
     5581 gaaaagacac cccgattcca ttcccgacct ccgcagcgtg tgcacgaggg agccgaccac
     5641 catgacgcag ccaccgatcc gccccggagt ccggccatga ccgaagacct ccacgcccgc
     5701 cccgcggtcg tgccgcctgg cttcttcgac ccgcccgatg aggacgacgc ccccgcccac
     5761 gcgtccgacg gggacgacgc gcccctcgcc gtccggcccg gccccctcgt cgatgccgac
     5821 ccgcaacgcc tgcgcgaact cctcgcccgc gtcgccgacg aggacgagga cgccctcgcg
     5881 gcactctacg aagccaccgg cagccgcgtg tacgggctgg ccctgcgcat cctgcgcgac
     5941 ccggccgccg ccgaggaggt caccgaggac gcctacttcc aggtatggcg ccaggcgcgc
     6001 cgctacgacc cggcccgcgg ccgccctctg gcctggctgc tcaccatcgt ccgcagccgc
     6061 gccctcgacc acctgcgccg ggacgatccc gccctgcccc acccggagcc cgtcagcctc
     6121 ctcgacgccg agccggaaca cacggacaat ccccagaacc tgctcgaagc ctgccgcgac
     6181 cacgaccggc tccacgccgc cctggccggg ctggacccgg tgccgcgcca gctcatcgcc
     6241 ctggtgttct tccgcggcct gacccacgaa gaggccgccg accacgcccg catgcctctg
     6301 ggcaccgtca aatcccatgt gcgacgcgcc ctgctcgccc tgcgtgccgt cctggccgcg
     6361 gacctcgacc ggagttacag cggatcatga agcccaccta ccccctcgac cccgaccagc
     6421 ccgacgcgag cgccgacgtg gaagcccgac tcctggaatc cctcaccccg ctgcccctgc
     6481 ccgaggcacg cggacgcgcc ctcgccagcc gtctgcagga acgcatccgc gccagccgcg
     6541 acgccggccg cggcctcatc accctgcggc gcgaccaggg ccactgggca ccgctggtgc
     6601 ccggcgtccg cgtcaagcgg ctgtccggtg gccagcgcgc cgtgctgctc gatctggcgc
     6661 ccggcgcggt gatccccttc caccgtcacc acgaagacga ggagtgtgtg gtgctgcggg
     6721 gctcggcgca gctgggcgac gtcaccgtac atcccggcga ctaccacctg gccaccaccg
     6781 gcagccgcca cggcgtcgtg cgctccgaaa ccggcgctct cctgttcctg ggcggcaccc
     6841 ccatcggcca cggcctggaa gtggcgcggg acgtggtcac ggccctgtta cccggtcgcg
     6901 gtcaagcccc cctcaccgtg ccgcgggacg agggcccctg gaccgatccg gcgccgggtc
     6961 tgcacctgaa actgctgcgc gacgacggcc acggccgctc gctgctcgtg cgcctggaag
     7021 caggctgccg ggccgatctg gcggagctgg ccggccacgg ggaatgtctg atggtggagg
     7081 gcgaagcctt cgccggcgac accctgctgc aggccggcga ctaccagctc gccccgcagg
     7141 gcgtgacgcg cccgaccctg agcagcgacg tgggcggact gctcttcgtg cgcaccggtc
     7201 tcagggtgtg aacaccccgt cgtcgtaggc ggcgcagaag gtcttccagt cggcgcgcaa
     7261 ctgcttcgca ctggcctcgg ccgccgccag cagggatttg cgccagcgcg cggcaccgaa
     7321 ctcgatgagc tggtcggcaa tggcggaacc gtcgcggccg ctgctacgca gctgggcgct
     7381 ggccacgatg tggcccatcg tggcaacggc ctcccgcagg gccggcagac cactgcgggc
     7441 cgcgtccagg gtgacccgat cctcggtggg ctggagttcg cgcagcaggt aggcgcgctt
     7501 gccgaggcgt tcgggcaaca ggaaggccat ggacaccgcc tgcatgcgcc gctgcaccgc
     7561 caccacccgg gccgcctcgc tcttccaggc cggctggggc gtcttgagca cgggcagcaa
     7621 ggccgagggc agcgcctcct tcaggtcgag gagatagtgg ccgtccggcg agcctttgcc
     7681 acgcaccagg atcacgaagc gatccacccc caggctgccg ttgccggcaa tccgccgggc
     7741 cacatcgagc acttcgtaga acgccgggtc gtcgcgggtc ctggccacgc cggccatgaa
     7801 ttgcgcgatc cgctcgcgct gtttttcagt gaccggcagg gctttgccgt tgtcgaggcg
     7861 gatcagacgc ttcttgccct tgcgcacggt tcggccgtcg agaaaggccg cacgctcccg
     7921 cttgcgcagg ccgtcgagga gctgatgcac cagcccggat gccgtctccc gctccaccca
     7981 cagggctttg ccggcgacca gacgttcccg gtaggcgtcg aggaagtcgc cgcacaaggc
     8041 cgccgcctcc ttgcgcttga cgcccaggga cggcgcgccc gcatggacgc tggtcaggaa
     8101 gcgcaccagc tcccagtggg ccggcgccag cgcgccctcg tcgaaatcgt tgaggtcgaa
     8161 gtacaccagc cggttgtcgc ccttgtagct gccgaagttc tgcaggtgca gatcaccgca
     8221 gcaccacacg gcaggggagt ccggcagctc cggcaggcgc cggtagaaga gatggcaggt
     8281 ggcgcgcagg aacacgaagg gatcgctgcg catgttgcgg aacttgagga ccaggcgctc
     8341 gggatcgcga ccggcattga aggcacggat ttcctcgacc gcatcgagca tggcgcatct
     8401 ccccggcatg tgaccagggc attctacgcg ccacgcgctc agtgatgagg gcgttcgtcg
     8461 tcgtcgccct cgcccggcgc cgggcgggga cgcggcgcgg cttccatctg cttcatgcgg
     8521 gcgatgcgca ccgtgcaatc gccggcgacg gtggcgcaaa gggcctcgtc gccgatgcgc
     8581 aggcgcttct gggtggcgcg gaagcggccg aggaattcgt aggcgctctg ttcggcgccg
     8641 cgcatgccaa ggcgggccga gttctgggca ccgacccagc cgaagtactc catgcccagg
     8701 cggcggggcg tgggacggcg ttcgtccttg agcagcgccg tgaggcagtc ctcgtagtcg
     8761 aaggtgtcgt ggtggatcag gcggtagcag cgggactggg cgtaaacagc cccctcggcg
     8821 accgcggtga gcggatcggc gggcgctgcc atggcggcgc cgctagccgc cagcagcagg
     8881 cagagcagac gtttcatgag gcctcctcaa acggcaaacc ggatgtcggc ccgcgccgac
     8941 acccggtcga tggggtccgc ccgctcagtt caggcgggtg atgcgcgccg ccgggggcgc
     9001 caggctggga tgagccttca tgtaaccgac gaaagcgtcg atgtcgatca cgccgctggg
     9061 ggtgacgttc ttgccgttgg cggtgaccac cgagaagttg tcgcccggcc cgggggccat
     9121 gaagctgttg agggtgacgc gataggtctt ggtgaggtcc atcgccacgc cgttgagctt
     9181 gagggagcca gccaccacgc ggttgccgga ccccaccgcc gcaccggccg gcttggaggc
     9241 atcccaggtg tagctgaagg tgctgctcgg ctggagcatg cggccgcagc cgttggcgcc
     9301 ggtcttggcg gcgcagttgg gggcttccca ctgctgctcc agcaggcgga cgaggtcggc
     9361 gccggtcaga tcgacggtca ccagagtgtt gccgaagggc gccacggtga ggaggtcgcc
     9421 gtaggtgacg gcaccgccgt tcttgtagat caggtcggcg cgcacaccgc ccggattgat
     9481 gaaggcgatg tcggccttgg ggccgccggc cagatacacg tcggccatca cgtcacccat
     9541 ggctccttcg gcggtttcgt cgcgggtcgg cgttgagctg ctggagagca gggcgcgctt
     9601 gatatcggca gtgatggagc cgaccacctg gttcttcagc gtggccgaca ggctcacgta
     9661 ggactgcacc gcggcgtcga tggtggcgtc cttggtgagg atggagaagc cggccggcac
     9721 cgtggtgttg aggtcgttga tgaccggccg ggtgttggcg tcgcgggtca gcacgccgcc
     9781 gccgggcagc agggtcaggt tgatctcggt gaccgcgctg ccatagaagc cggtggaggt
     9841 gagcagtttg ccattgcggg tgcacacgta ttcctggtgg gtgtgaccgc tcaccaccac
     9901 atccacattc gaactcaagg catcgacgat gggcaggatg tcgccagtga ggttcgggca
     9961 ggtcttgtcg ttgagggtcg aggcggtggt ctggccgccc tggtggatca gcaccaccac
    10021 cgcattcacg ccgctggcct tgaggctggc cgcgtacttg ttgatggtgg tcgcttcatc
    10081 ctggaagtcc agcccggcca cgccggtgga ggtcaccacc tggggcgtgc ccttgagagt
    10141 caggccgatg aaacccacgg acacggggcc gaacttcttc acataagtgg ccggaaacag
    10201 cgattggccg gaactgctgt ccaccacgtt ggccgacagc catttgtagg tggcaccgct
    10261 gaagctgccg cccttgatgc aggtatcctg gccgatggtg ccgccggggt agcagccgcc
    10321 attctgcagg cgcagcagct cggtcttgcc gtggtcgaac tcgtggttgc ccacggaggt
    10381 cacttccagg ccgatctggt tgaggatgtc caccgtggcc tcgtcgtggg tcagggtgga
    10441 ggtgaccggc gaggccccca ccatgtcgcc ggcgccgacg acgatgctgt aggggttctg
    10501 ggccttgagc tgcttgatca gcgtggcgag ataggccgcg ccgccggaac gcaccgtggt
    10561 gccggccgcg ttggccgggt ccttgagcac caccgagccg ccgttgttgg ccgccggcac
    10621 ctcgatgttg ccgtggaagt cgttcatggc gatcagcttg acgctcaccg gcgccgccga
    10681 ttcactgctg ctggcgccgc cgccgcagcc cgtcaccgcc cacgcggtgc tcagggctgc
    10741 cagccaggcg gccatcttcg tgctgacagg acgcttcatt tcgatgcctc ggtcggtgcg
    10801 aaaaataaac ccgcacgcta ccggcccaat gtgaaacgcc tgtgaagccc cctcagagga
    10861 gcagattcac cccgtgcagc agaatccggc aggccaccgc aatgcccagc gccactgcca
    10921 ccgacaagcc cagggagaac accgcctcac gccgcccgac ggtcttcagc atgaccgcga
    10981 aggtggacac gcagggcacg tagaaagtga tgaagaccag gaaggtggcg atctgcaccc
    11041 agtccagcag gggcgcgatc tcctgggtgc cgagggcctg gtagaccatc agcagggaca
    11101 gctccttgcg cagcacgccg aacaggatcg gcactcccag caccaccggc aggcccaggc
    11161 tccaactggt caggggcacc agtaccgtgt tgatgacctc gtccgcgcca aaatgggtca
    11221 gcagcgccag caccacgctg cccagcacca gcagcggcgt gacgatggtg aggatgtcgc
    11281 tggtgcgttc ccaggtcacc gccagcacct tgcgccagcg gggcagggcg taagccggaa
    11341 tcgcctggat catcccgggg ctggcctgca cgtaacggcg ggtaagcaga cgcccgccga
    11401 ccgcgatgac cagcatggtc agcaggaaga tgcccagcac cccctcccag cccagatact
    11461 tgccaccgat ggccagcacg atggccgagc gcgccgagca gggcacgaag gtgatcagca
    11521 gcgaggccac gatgcgctcg cgccccgccg agctggccgc caccgccgac agggccggca
    11581 cgttgcaccc cagaccgagc aggaagggca ccgccacgcc gccatgcagg ccgatgcggt
    11641 ggaaaccgcg gtccaccacg aaggccaccc ggtgcatgat gccggactct tccagcccca
    11701 ccagcagcag caccagcggc agcatgtagg gcaccacgat gcccaccagc cccaccagcc
    11761 cgtcggcgat ggcgcgaccg atcaccccgg cggtagtgtc cggctgccat tccccgaccc
    11821 acgccgccag cggcgccgag gtaacggaat cgagcccggt gctcacctcg aagaccacgt
    11881 agagcaccag ggcgaacacc gccagggtgc cgatcaggcc ccagcgcgga tgcaggaaga
    11941 gccgatcaag ctggcgcttc cagccggcct tctcggccgc gtggccgagg cgcgtgacct
    12001 gctcgtggag cagcgcggcg cggtagtggc ggtcggcaaa caattcttcc gaaagggaac
    12061 ggggcagctg cgcgcccgcc gccttccgcg ccgccgcgat gcgcggcatc agggccggga
    12121 agcgctcggc aaactcccgc gccagcaggg tgtccgcctt ggccagctgg gtcagcagca
    12181 gggcccgcgg caccggaaag gcggcctcca cctcgggcag gtcgaggacc gcgtcaaacc
    12241 cgtgcaaggc ctgacgcagg tgcgggctgg ccggctgggg cacggggcac accgaccggc
    12301 gcacggcctc cagggccgcc tcgaagaggg ccgagatgcc cttgcccatg tgggccacgg
    12361 tgggcaccac ggtcacgccg agggcctcgg cgagggccgc tgtgttgatg tagataccct
    12421 tctcacgggc ctcgtcgagg cggttgaggg cgatcaccag gggcttgccc agcaggcaca
    12481 gctcctgact cagggccagg tcgcgggtca gggccgtggc gtccaccaca tgtacgagga
    12541 cgtcgggtgc ccggaagccc ccttcacccg gctcgccctc cagcagcgcg gtgagcaccc
    12601 gccggtcggc ttcgtccaga tcgagcaggg tgcgcagggg cggcagatcc accaccccgg
    12661 cctgctccag gcccacattg acgacgcacg cgcggtagcc cagcgcactc ccgtccagtt
    12721 gacccgactg cacggccgta ctcgatgccg catcgaacag ggtgctcttg ccggtccgcg
    12781 gcaggcccac cagggccacc accgggcggc agcgtccacg cagcagacgc gagggaacgg
    12841 cgacacggac ctccgggggc agggcggcac tcatgatccg ctccgcccgg ccgtaaatgc
    12901 catcgaaata tgcctgtcaa ccaccccgta ccaccgtacc acgccggtca tgatgcgacg
    12961 ccccaccgtt caggagcgat cattatattg gtaataagaa ttgttcgcac ctataaacac
    13021 gcacgctgca cagggtcttt gtgtagcacg tggcgcgcgc gctcagaccc gcaggccggc
    13081 gcgagtcatc agcagcaggg cctgggcacg gctggtgacg ccgagggagc gcaggatcgc
    13141 cgagacgtgc accttgacgg tgccttcgga caggccgagc agatcggcga tatcccggtt
    13201 ggacttgccc tgggccacca actccagcac ccgcccctgg gcggcggtga gctggaaacg
    13261 ctcggcaaag gcctggctgg cccgccgcgt acggccgctg ccgtccgaac ccgacgtggc
    13321 ctccggcgtg aacacgccac cgtccagcac cgtccgcacg gcctcgatga gcgtgttgct
    13381 ggggctggcc ttggagacga agcccgaagc tccggccttc atggcgcggt gcacggactc
    13441 ccggtcgtcc agggccgaca ccaccagcgc cgggacatcg gggaagcgct tggcgatgac
    13501 accgagcagg gagaagccat tcatctccgg cagcatgagg tccaccacca gcagatcgca
    13561 gtcgggatcc tcctccagca gggcaatggc ctcggcggcg ctgctggcct gctggcagaa
    13621 gacgccttcg cccacctggg cgagggtcag agccatggct tcccggacga ggacgtgatc
    13681 ttcaatgatc agaatacggg tcggcatcat ggcaaacggg cgctggaatc gcggcagaac
    13741 ctgaggtcag gccagtttaa cctaccctct ggaggggaaa aaatagaaaa tccgggcctt
    13801 ccctgccaaa gaaatcaata ggacccatca tgccgcgtat tgcctttgcc ccgttcaacg
    13861 cagccggcct cctgaccagc ctgttttccc tgctcacccc gtcggcggcc ggcgccgccc
    13921 ccaacggcga ggacagcttc ctggacgacc tgccggtggt cctgtcagcc acccggttga
    13981 cccaggcgcc agccgacgcg ccgggttcgg tgacggtcct ggaccgggac atgatccgcg
    14041 cgtcgggggc ccgcaacatc agcgagctgt tccgcctggt gcccggcttc caggtggcca
    14101 tgcagaccgg caacacgccc caggtcacct atcacgggct ctcggacgaa gcgccgcggc
    14161 gcatgctggt gcaggtggat gggcgctccg tgtattccgc ctatttcatc tccggcgtgg
    14221 aatggaacca gatcggcgtg gatctggacg acatcgagcg catcgaggtc tttcgcggtt
    14281 ccaactccgc cgcctacggc agcaacgcct tcctgggcgt ggccaacatc atcacgcgcc
    14341 ccgcggccga gacccagggc gtcgcactgc gcctgcgcgg gggaagcgat ggcgtgaacg
    14401 atcagggcgt gcgcatcggt cagcgcctag gtgacgtgga tctgcgcgtg accgctgcgc
    14461 gcagccacga caacggcttt gccgacctca atgactggcg ccgcaacgaa ctggccaacc
    14521 tgcgcgccga ctggcacatc cagcacgatc accaggtgga attccaggcc ggctggaccc
    14581 gcaacgagtt cggcaccggc aaggccaaca acctctccga tccggagcgc agcgccgaac
    14641 tgtccacagg cttcggcctg ctgcgctggc gccacacgcc ccagccggac gaggaactgt
    14701 cggtcacgta ttaccaccag caggagaccg gcaaggacgc ctatccctac accgtcatcg
    14761 tcccgcctca cctcaccggc ctgccggtga atctgcagat ccccttcttc ttcgaatacg
    14821 gctacaacgt cacccgcgac gatctggaag cgcagcgcat caccagcctg tcaccgacgc
    14881 tgcgtgccgt ctatggcggc ggcctgcgct ccgaccgcag ccatgcgccc ctgcgcttca
    14941 atacggccga caccgtgcgc agcgacaccc ggcacctgtt cggcaccctc gaatggcgcg
    15001 cctctccggc ctggctcttc aacctgggcg cgatgctgga gaacagctcc ctcaccggca
    15061 cctcggtgga tccgcggatc tccgccaact accatttcag cgacatctcc acctggcgcg
    15121 cgtccatcaa ccgctcccac cgtcacccca gcgccttcga acagaaggcc aacatggtct
    15181 tccgcaattc ggccgccttc cccaccccca tcggggccgt gccggcgggc accgtgatct
    15241 cccagacctt ccgcccctcg ccggacatca ccgcggaacg catcaccacc tacgagatcg
    15301 gctacctcgc cgagttgcgt tccctcgcca ccacgatcga cgctcgggcc ttcgtggagc
    15361 gggcacgcaa cctcatcgag atgaacatcg agaactccac catcggcctc gtcccgcggg
    15421 acaacgtccg ctacttcgcc aacacgggcg aagccgacat ccgtggcgtc gaactgtccg
    15481 gcacctggcg tcccgccgcc cagacctggg tcaacctgac ccacaccgaa ctgcgcatcg
    15541 ccggccccac cgtcaccgcc gcccaggccg cccgccagtc cacgccctac gtcagcaaca
    15601 ccgcgcccaa gcacacctcc acggtcttcg cagcctggga gttcgtgccc cgctggcagg
    15661 tcagcgccag caagcgctgg gtcggctcga tgagctggta ccaggacgac ccccacgcga
    15721 tccccatgta tcgccagctc gacgtgcgca tcgcccaccg cttccagacc gcctacggcc
    15781 gcggcgaggt cgccctggtc ggccagaaca tcgacggcgg cgaacagacc ttcacgtcca
    15841 ccgccggcgt gtggggcagc cggaccttcg ccaccctcag cctggagttg tagtcgcccc
    15901 ttcatgggat gcacgatccg ccgcttcctg ctgatcctgc tgaccctcgc cggtctggcc
    15961 tcccctgccc gggcggcgct ggtggcactg gtgagcgccg atgaaggcgc cgccttcagc
    16021 gaagccgccg acgcgatcac cgccgaactg cggggcgccg gccatcgcgt ccagagcttc
    16081 acgctgcccc tccgcgcgga cgacacgggc ttcgcctccg cggcgctgat cgtcaccctt
    16141 ggcagccgtg ccgcccagct cgtcagcacc ctgccccagc acggcccggt gctccacacg
    16201 ctgctgccgc gcagcgcctt cgaccgcctg ccgcggcccg acgacccccg ccgcgtctcc
    16261 gccgtctata tcgaccagcc cgccggccgc cagctcgaac tgatccgcca ggctctgccc
    16321 gactggcagc gcctggccgt catcaccggc cgcgacagcg ccgaactcgg cacccgtctg
    16381 caggccgccg cccgcgaaca tcggtccctg cgcgtgcaga ccgacatcgc cctcggcgag
    16441 cgcgacatct accctgtcct gcagcgcatg ctggccgagc cggccatcct ggtcgccgtg
    16501 ccggacgcca ccctgttcaa cagcaacacg atctccaaca tcctcctcac ggcctaccac
    16561 caccgctcgc cggtggtcgg cttttctgcc gcctacatca aggccggtgc cctcctggcc
    16621 ctctacagca cgccggccca gatcggccag caggccggcg aagtggcccg ccaggcgctg
    16681 gccagcggca ccctcccgcc gccccagtac ccgcggcagt tccgcgtcgg caccaatgcc
    16741 tacgtggccc gctccctcgg catcacgctc gaagacccca acgccttgcg tgatcgcctc
    16801 gaacgcacgg agggcacgcc atgagcgccc gcgatctcga catcggcacc cgggccctgg
    16861 tgatcgcggt gctgcccgct ttcgtcgtcg cggtggtgct gatcgcctat ttcacccaca
    16921 gccgcctcaa cgacctggaa gacgcccaca gccagcgcgg caaggccctc gcccggcagc
    16981 tggccgcggc gagctcctac gtggtattca ccggcaacaa ggacgccctc ggcaagctcg
    17041 ccgccgaaat ccttgacgaa cccgatgtcg tccgggtcgc cgtgcgcggc cccggcaagg
    17101 acaccctgac cgatctgcgc aagcccgccc cttccgacag cggcgccctg cgcctcggca
    17161 acgccgactc cgacacactc gccttccagg aagccatcca cagccccggc cagctcttcg
    17221 acgacccctt cgtcggcgcc gagggcgccg gcagcgagct ccacgcccag cagggcgaag
    17281 tcatcgtcga actgtcccgg gacgccctcc acggcgaaga gacccgcctg ctgcgcaatg
    17341 ccgtgctgat ggtgttcgcg gtgctgctgg gcagcgtggt cctcgccctg cgcctgagcc
    17401 gcggcatcac ccgaccgatc cagcgcatcg cctccaccgt ggcccgcatc ggccagggcg
    17461 aacgtgccgc ccgggtgcgc gtcgaaggcg gccacaccct gcgcgccctt ggtcagggcg
    17521 tgaacgacat ggccgaccgc atcgccgctg ccagcgagaa tctggagcgc cagatcgaag
    17581 ccgccaccgc cgaactgctt gaaaagaaaa acgaggccga actcgccaac caggccaaga
    17641 cccgcttcct tgccgccgcc agccacgacc tgcgccagcc catgcacgcc ctcggcctgt
    17701 tcgtcgccga actgggccag aagccccacg cccccgacac ctgcacgctg gtggagcaga
    17761 tcgccatgtc cgccgagacg atggaagacc tcctcgacag cctgctcgac atctcccgcc
    17821 tcgatgccgg cgcactcgaa ccccagatca agcccttccc gctgcagccg ctcctggagc
    17881 gggtggacgt ggactaccgc cgcgaagcgg aacggcgcgg ccagcgcctg cgcctgcgcg
    17941 ccaccgatct gtgggtcagc agcgacccgc tgctgctgga acgcatcctc cacaacctca
    18001 tcagcaacgc cctgcgttat gcgccgggcg gcaccgtgct ggtggcctgc cggcgccgcg
    18061 gcgcccaggt gaccgtggaa gtgcgcgaca acggcccggg catcgccccg gacgcccagg
    18121 aggccatctt tcacgagttc gtccagctcg acaatcccga gcgcaaccgt gccaagggcc
    18181 tgggtctcgg cctcgccatc gtgcgccgtc tcaccaccct gctcggtcac cccctcggcc
    18241 tgcgctccag gcccggccgt ggcgcactgt tctcggtcac cctgccggtc accgcgcccc
    18301 ggcctcagga cctcagcccg ccatcgtccg cggcgcgggg cctcgccggc ctgcatgtgg
    18361 ccctcatcga cgacgacccc ctcgctgccg ccagcaccct gagcctgctc gaatcctggg
    18421 gctgcctggt caccgcggcc gataccctgg agcacatgta cgccgccctg cagcgcagcg
    18481 agatgagccc ggacatcatg ctctgcgacc accacctgga cggcgccctc gacggcgtcg
    18541 cggtggtcgc ccgcctccgg cagctcctcg gcaccgatct gaccgccatc atcctgagca
    18601 gcgacaccac ccttgccgac tcgccccgcg tccgcgacaa cggcatccgc gtcctgcgca
    18661 agccggtccg cccggcccgc ctgcgcgcct tgctgcaacg caagacgggc agctgatgca
    18721 gcgtccgcac gcatcggtta agatgcccgc accacgacaa ccacgtacaa gagggggagc
    18781 caccatgagc gccgacaaca ccatgcagga cccgatggaa ttcatgcgca acatgtggag
    18841 caagatgggc ttcagcctgc ccggcatggt cacgccaacc ctcgacctgg aagagctgga
    18901 ccgccgcatc aacgacatga agaccgtcga gaactggctc aagatgaacc tcagctccct
    18961 gcagatggcc acccagggcc tcgagatgca gcgcgccacc ctggccgcca tgcaggccat
    19021 gagcaaggtc gccaccgaag gcgccggcaa ggtcgacgag ggcaccccca accccttcgc
    19081 cagcaccatg tggccgtgga acttcgttcc ggccgcggcc gaagccgcca aggccgctgc
    19141 ggccccggcc gagagcgccg agccggccgc caagcccgcc gccaagagca agagcgccaa
    19201 gtaagtccgc cgtccgcgtg cgcccggcgc cgtgccgggt gccgcccctc atcccgcggc
    19261 cacaccccgc agggccgtca gccagcccgt cagcgtcacc gccgccgcca ccgtcgtggc
    19321 cgagatcagc caggccactc cgggcccgtc accgcccatg cgcatcgcca gaatgtaggc
    19381 cgacgatgcc gtcggcaggg ccgcgaacag caccgccgtc gcaaaagcca gcccttccag
    19441 cccgaaggcc cccgccagcc cccacgccac cgccggcagg gcgagcagct tcaccgcgca
    19501 cagatagacc atgcccacag gccggccctc ccgctgaccg atccgcagcg ccgcacccac
    19561 cgcgatcaga cccagggtca ccgccgcatc cgccagccgt tgcaggaagg gcagcaccgg
    19621 ccccggcaga tgcagcccgg cggtgctgaa ggccagcccc gccagcgtcg cgatcaccag
    19681 cggattgcgc gccagttccc gcagcagccg accctggccg tggtgggcca ggaagcccac
    19741 cgccgccatg ttcgcaaagg gcaccatcgc gccgcacagc gcccccatcg ccgccacacc
    19801 tgccgcgccg tggagggtgc ccgccaccgc gatgccgatg taggtgttga agcggtaggc
    19861 gcactgcacc cgcgacgcaa aggccatctc ccccaatccc atcagcggcc gcccgaggag
    19921 cgccagcgcg aagccggacg ccatcgtcag caacccgacc gcgaacagcg gcgccgccgc
    19981 cgacaggtcg atgggcgccc gcgccagggc atggaacagc agggccggga agaacacgaa
    20041 atacaccagc ttctccaccc cggcccagaa gccgtcctcc agccgcccga agcggcgcag
    20101 caccacgccc agggcgatca gggcgaaatc cgggaggagc agcaggaagc tttgcatggg
    20161 cgcacggacg gggcggagac gggtccggcg agtgtgccag aagccggact gccggcgaaa
    20221 tccggaccgt ccgcacacgc cgcgcgccgc acggcgttcc ggggcaccgc acaggatctg
    20281 cccgagcgcc tgcgccgctc acgccagcgc tgaccgccgg acgctgcgga cgcgcgtcct
    20341 gccggcgttt acgcgtagcg ccgcacgtcg tcgatcacct tgccgtcgtt gggcaggctg
    20401 cccggcgcgc agcactgcac ctcgccgcgc agcttggtga gctcccgcag gctcgcgacc
    20461 accgcctctg ccagcccggc cccgtcgccg gccacttcgc agtggagcgt catgcggtcg
    20521 ttcagatccg ggttgtccac caccagacgg ccgcgcagga tctccggatg gcgcttgagc
    20581 accgccgcca cctggccggg atggacgaac atgcccttca ccttggtggt ctgatccgcc
    20641 cgtccgagcc agccgcggat gcgcgcattg gtccggccgc agggcgacgc gccgggcagg
    20701 atcgccgaca ggtcgccggt gccgaagcgg atcagcgggt aatccgggtt gaaggtggtc
    20761 accaccacct cgcccacctc gcccggcgcc accggatcgc cggtgccggg gcggacgatc
    20821 tcgacgatca cgtcctcgtc caccaccatg ccctcccgcg ccggggtctc gtaggcgacc
    20881 agcccgaggt ccgccgtggc gtaggcctgg aaggcctgga tgccggccgc ggcgagggcg
    20941 tcgcgctggc tgggcggaaa ggcctcgccg gagacgaagg ccttcttcag actgtccagg
    21001 cgcaggccct gctcggccgc cttgtccagc aggatgcgca ggaaggacgg cgtgccggcg
    21061 tacgcgtcgg ggcgcaggtc cagcatcgcc gcgagctgct gctcggtctg cccgaccccg
    21121 gcggggaaca ccgtgcagcc cagggcgtgg gccgccgtct ccatcatcga accggccggc
    21181 gtgaagtggt aggagaaggt gttgtgcacc aggtcgcccg cccggaagcc cgccgcgtag
    21241 aaggcccgtg ccaggcgcca gtaatccttg cgcgcgccct ccggctcgta gatcggcccc
    21301 ggcgaggcga acacccgcgc acagccggcg ccccaaccca gcgccgccag gcccccgaag
    21361 ggccgcgccg ccttctgcag cgccgacagc tcggacttgc ggatcaccgg caggcttgcc
    21421 agcgcctcac gggacgtgac cgccgccgcg tccacctccg ccagcaggcg cgcaaaggcc
    21481 ggcgtacgcg cctgggcgtg ggccacctgg accggcaggc gggcaaggag atcgcgctcc
    21541 cgctcggcgg gatcgcgggt ttcacgggcg tcgtagtagt tcatggggtc tccgcgt
//
LOCUS       DAOQMM010000059        21574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_837_length_21574_cov_48.271899, whole genome shotgun sequence.
ACCESSION   DAOQMM010000059 DAOQMM010000000
VERSION     DAOQMM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21574
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_837_length_21574_cov_48.271899"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLH85_10600"
     CDS             <1..528
                     /locus_tag="PLH85_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB65059.1"
                     /translation="AAAEFLGTTLAERGIELVYGGGNVGLMGVVADACLAAGGRVVGV
                     IPRALLDWEVGHEGLTRLEVVDSMHTRKARMAELADGFIALPGGLGTFEELFEILTWA
                     QLGFHAKPVGLLDTAGYYLPLLQMIERGVAEGFMKAENRGLLLAEDNLFALLRAMGQY
                     HPPTVSKRIREEKHL"
     gene            583..2190
                     /locus_tag="PLH85_10605"
     CDS             583..2190
                     /locus_tag="PLH85_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNB65060.1"
                     /translation="MSVIRSSINPRSAEFQANAAALRTLVADLRDTVGRVALGGGAAA
                     RDKHTARGKLLPRDRVDHLLDPGAPFLEIGQLAAWGLYDNEAPCAGLIAGIGRVSGVE
                     CMILANDATVKGGTYYPLTVKKHLRAQEIAEQNGLPCVYLVDSGGAFLPRQDEVFPDR
                     DHFGRIFYNQANLSARGIPQIAVVMGSCTAGGAYVPAMSDETVIVRNQGTIFLGGPPL
                     VKAATGEEVSAEDLGGGDVHTRISGVADHLAENDAHALYIARRIVANLNRVKPVALRL
                     ADAEEPLYDPEEIYGILPSDTRKPYDVREIIARVVDGSRFDEFKARYGTTLVCGFAHL
                     YGYPVGIVANNGILFGESAQKGAHFIELCAQRGIPLVFLQNITGFMVGRKYENGGIAK
                     DGAKMVTAVATAQVPKFTVLIGGSFGAGNYGMCGRAYSPRFLWMWPNSRISVMGGEQA
                     ASVLATVRRDGIEARGGHWSRDEEEAFKSPIRQQYEDQGHPYYATARLWDDGVIDPAQ
                     TRRILGLSLSASLNTPILPTRFGVFRM"
     gene            2201..2992
                     /locus_tag="PLH85_10610"
     CDS             2201..2992
                     /locus_tag="PLH85_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB65061.1"
                     /translation="MTYETLEIERAGAVATVWMNRPQVHNAFNETLIAELGGAWAALD
                     ADDAVRVVVLAGRGRSFSAGADLAWMQRAGAASVDDNLADAGRLAGMLRGLAELSKPT
                     VARVHGAALGGGMGLAAACDLCVAGSGAVFATSEVRLGLIPAAIGPYVIRALGERQAY
                     RYFLTAERIDARRAAALGLAHEAVEADALDTRVRDLCDALLLGGPQAQRAAKALIRAV
                     AKRPVDDALVADTAHRIATLRATPEAREGLDAFLAKRPAAWVPGA"
     gene            3000..3671
                     /locus_tag="PLH85_10615"
     CDS             3000..3671
                     /locus_tag="PLH85_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNB65062.1"
                     /translation="MDWTPAGLAVAAEGLRPLLDGGGIPLDLASLTALAAGLGWASGL
                     RLYALVFVLGALGRFAGVSLPGGLAVLSHPLVLGLSGLLLLVEFGADKLPWLDSVWDA
                     VHTFIRIPAGAALAAAVMGDQGGAAQVALGLLGGTLAAGTHFAKAGTRAALNTSPEPV
                     SNVAASVGEDALFAGGLWTLLHHPLVFLAALALFAVVAGLVLRALWGVMKKVLRPRMR
                     PAAPR"
     gene            3699..5696
                     /locus_tag="PLH85_10620"
     CDS             3699..5696
                     /locus_tag="PLH85_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNB65063.1"
                     /translation="MFDKILIANRGEIACRVIKTARRLGIRTVAVYSEADAGARHVRL
                     ADEAVLIGPAAARESYLVIERIIEAAQRTGAQAIHPGYGFLSENEAFCEACEAAGIVF
                     IGPPVAAIRAMGSKSEAKKLMEQAGVPLTPGYHGDDQTPATLQREADAIGYPVLIKAA
                     AGGGGKGMRRVECSADFLDALASCKREAASSFGDDHVLVEKYILRPRHIEIQVFGDSH
                     GHAVYLFERDCSVQRRHQKVLEEAPAPGMTPERRAAMGRAAVEAARAVGYVGAGTVEF
                     IAQQDGSFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGEPLPLAQEQLALNGHA
                     LEARIYAEDPGKGFLPATGRLLHLAPPDEGLHVRVDTGVEEGDEITPHYDPMIAKLIV
                     WDVNRDRALARMLQALADYRVAGVANNIEFLSRLTACPAFANADLDTGLIEREQAYLF
                     PEASAPERDEWLALALAELLREQERAAATAARSADPSSPWHARDGWRMNAAPARSLIF
                     RHGEAQKTLRVATLPGAFEITLDGVATRVSGSLNPRGLLRGEFGGRRLAATVVAAGER
                     RHVFAHGRARVFACVDPLFFSGEGGGAEGGLLAPMPGKVIALLAAEGARVDKGAPLLI
                     LEAMKMEHTIAAPTAGTVKGFRFAVGDQVADGAELVEFDAA"
     gene            5708..6631
                     /locus_tag="PLH85_10625"
     CDS             5708..6631
                     /locus_tag="PLH85_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNB65064.1"
                     /translation="MTLPARVRIVEVGPRDGLQNEAQVIATDTKVALIDRLARAGLPA
                     VEATSFVSPKWVPQMGDAAEVLARIERLPGVDYPVLTPNLKGFDAALAAGAKSVAVFA
                     AASESFSRRNINCSVAESLARFEPIMAAADEAGVRVRGYVSCVLGCPYEGAVAPSAVA
                     EVAGTLYDMGCYEVSLGDTIGTGTPGAARRMLDAVAQRVPVEQLAGHYHDTYGQALAN
                     VYASLQAGVAVFDASVGGLGGCPYAAGASGNLATEDLVYLLNGLGIASGVDLEALVDI
                     AAWISAQLGRPPASRVARASLAHRGRRGCAA"
     gene            6689..6916
                     /locus_tag="PLH85_10630"
     CDS             6689..6916
                     /locus_tag="PLH85_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNB65065.1"
                     /translation="MSVASPCTGICRIDPMTGWCAGCRRTLTEIAEWGAAGDGRRQAI
                     LDGLAQRRWPEGAPADKASADVVPPPAASTP"
     gene            complement(6966..7565)
                     /locus_tag="PLH85_10635"
     CDS             complement(6966..7565)
                     /locus_tag="PLH85_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB65066.1"
                     /translation="MKIKTLAAALLSLSALHAHATVIDFNQVIGFQTLPSPYQEDGFT
                     ITNSSTNSSAMLFWGTLSYNADPNGNTYSHNYGGTTSTLSKTGGGSFTFNSIDLTDVY
                     NFGSGGSVVFDFLYSNNSTSHSTVNLPTVAGLHTFTFDLANLTSVSWTPVSTQGNWIQ
                     VDNIVLDTTLSNRVPEPTSMALVGLGVAMAGALRRRRAA"
     gene            7850..8017
                     /locus_tag="PLH85_10640"
     CDS             7850..8017
                     /locus_tag="PLH85_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNB65067.1"
                     /translation="MDTSDENPCVGICEIDESGYCVGCGRSADEIYGDSEPAPCATDT
                     LPAVEAPPPDA"
     gene            8173..9015
                     /gene="rapZ"
                     /locus_tag="PLH85_10645"
     CDS             8173..9015
                     /gene="rapZ"
                     /locus_tag="PLH85_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNB65068.1"
                     /translation="MQLVLISGLSGSGKSVALNVMEDSGHYVVDNLPSTLLPQLISNL
                     RGGGYQRVAVAVDVRSGASIAALPQQVALLRGMVTDLRFLFLEARDETLIARFSETRR
                     KHPLGDECSTLAEAIGREREALALISELGQRIDTSDISANTLRNWVKSFMDLEASSQI
                     TLLFQSFGFKHGIPVDADLVFDVRCLPNPHYNPELRPLTGLDAPVIDFLERVPEVGRM
                     VEDIYAFVASWLPAYVHDNRSYLTIAIGCTGGQHRSVYVAEQLAGRFRESARVLVRHR
                     SFRR"
     gene            9012..9419
                     /locus_tag="PLH85_10650"
     CDS             9012..9419
                     /locus_tag="PLH85_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNB65069.1"
                     /translation="MSRLADLRTWLAAWVAYLRVGDVLVLALGLGASAASALVLWQGG
                     APDKAVIKAGGRVFAEVDLSAPRIVEVPGPIGTTRIEIQPGRARVAADPGPRQYCVRQ
                     GWLHSAGAVAICAPNEVSLSLSGRNAAYDSLNY"
     gene            9400..9930
                     /locus_tag="PLH85_10655"
     CDS             9400..9930
                     /locus_tag="PLH85_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNB65070.1"
                     /translation="MTPSTTEIRLVPTADDHRVARHAAAAIVLTVAEAAIPLPLPGVK
                     PGLANIITLVVLARWGWRDAAWVAILRILAGSLLLGQFLAPGFFLSLAGGLASLAVLG
                     AAMHLPRRWFGPVTQSVLAAFAHIGGQVLVARLWLVPHDGVFYLVPVFALAATVFGLG
                     NGLIAARLLAEEVPGR"
     gene            complement(9932..10456)
                     /locus_tag="PLH85_10660"
     CDS             complement(9932..10456)
                     /locus_tag="PLH85_10660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNB65071.1"
                     /translation="MMNSRQKPLSPPVVEGDAPLRPDARDLWQLWFDGAAHPNPGRLG
                     LGGVLIAPDGRRHAYSALAPECGCNNEAELLALGRGLELARGAGATHVQVWGDSDFAV
                     RHLNGAAHTDIPRLRALLDVIGRQLTGFTLVHLHWIPRHRNHDADRLSRAALGLPDKP
                     APRPVSARKRRGRR"
     gene            10753..12468
                     /locus_tag="PLH85_10665"
     CDS             10753..12468
                     /locus_tag="PLH85_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65072.1"
                     /translation="MRTLAGRAPGGEPWAALFAEALSEADPDAAALVGAAAVDGIVAD
                     GRACAQLMEWEARLAALATRPCGGPSAQAAAALALRRGLVALVLRGDAQAAWTIWDDG
                     VHQAERAASEPLQLALTGARGVAECLAGAVTRADVLVSDAWVLCEVKARSRLPCLLVT
                     VALSLIHLMMDRSSDALALLQRTCAAAAFEGLPESLQVLVWSLGMHAAADVGDTDRVA
                     DWSRAVADRTVPVPRLLLHACRHYVLGAMALRQGDALRARIHADACIGVGERAGALLP
                     GYIGRLLRLQALADLADAGASLRTEAAQWIPTWSACGMARLAAVAHLELAARDVRAGE
                     LDRARRAWFAALAALPPGESLRPLNRSGVWLEALRVRLFPMGDRCDATTAAVRIRTLG
                     EFSVEIGGRRIFDRDWKGSRTKTLLMALICEGGSKVSVDRLADLLWPDSDGAQARQNL
                     KVALWRLRRLGCEGPPEVNWVHVKQGRVSLPAGLVQVDALEFRNVARAALMPGADVGL
                     MREALAAQAESFLPMEDNLPWVAAFRKGLMGLKQAVQARIAGCGEQGAVRVVGEACTS
                     ELNGA"
     gene            12671..13414
                     /locus_tag="PLH85_10670"
     CDS             12671..13414
                     /locus_tag="PLH85_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65073.1"
                     /translation="MTDILRAMRIVVASLLVSGLVGAANAGPYQTYPLSARTDYLWMA
                     TDNATLGENWIRGLDGNNPAAVDAQHPYGNSATTALAAEDMMWYCPPGGCASNTRTGL
                     AYFSTVFNLNSDFRVAGGQLKIIADDYFELYLNGTLITFGLLDMHMDASGQPVPIILD
                     LSLYKDDFYGGENLIAIKAMDGYQMSPGGVCSGTTMQTQVGDFCQSARGFEYVYVSGA
                     IDTIPEPPVGSLMVFSMAALAAMRRFGRS"
     gene            complement(13558..14412)
                     /locus_tag="PLH85_10675"
     CDS             complement(13558..14412)
                     /locus_tag="PLH85_10675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HNB65074.1"
                     /translation="MNTPQAGILLPVPPLARYLGFTLEPDSDVHAGLSALLDVVDGER
                     TVAGLGLATVTAAGLSVPGLHAFPTPSAPGIELPATPAALWLWLRGEDRGELHLRAQR
                     IEALLAPVFRLVDSIDAFKHDSGRDLSGYEDGTENPQGDDATTAAIAADGSSLVAVQR
                     WLHDFRRLAAMAPEARDDCIGRRRSDNEELDDAPASAHVKRTAQESFSPEAFLLRRSM
                     PWSEGRDAGLMFVAFGATLDPFEAQLRRMTGQEDGIVDALFRFTRPLTGAYFWCPPVR
                     DGKLCLIR"
     gene            14615..15397
                     /locus_tag="PLH85_10680"
     CDS             14615..15397
                     /locus_tag="PLH85_10680"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB65075.1"
                     /translation="MQSFNSPRAVLTALLMSAGLGTAWAGPVVSTPFDLEISSTPFVT
                     GTAYAGVSVNTTASVSTPLDVNGRAWTDLQYVEDPLVWTGVRYNYPAPSSPQIATDPT
                     HCSSSRPGLCSYIWYDPNHTSSGTNGVTRAYFRTTFNLPEFTGDAAPLGAVLRVRADD
                     DFAVWINGFLVMLNADYGTSGDKGPDYVFERGVSQYLRQGELNVMTFVATDGSLNSPS
                     NVSYEHLAFELKLNNQVPEPASLMLGLMGLGGIGLARRRRTG"
     gene            complement(15474..16061)
                     /gene="metW"
                     /locus_tag="PLH85_10685"
     CDS             complement(15474..16061)
                     /gene="metW"
                     /locus_tag="PLH85_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNB65076.1"
                     /translation="MMSLRYDYDVIANWIEPGERVLDLGCGDGSLLRHLGEVRRIRGY
                     GVENDPERVLACARNGVNVIQIDLEEGLAGFENGFFDHVIMSLSLQAMHNTQRILQEM
                     LRVGREAVVSFPNFAYWRHRQAIINGHMPVSDSLPHQWYNTPNVRFFTIADFDDLCAR
                     EGIAIHERLAFDDHKVVLEEPNFLASVALYRLGRA"
     gene            complement(16069..17193)
                     /locus_tag="PLH85_10690"
     CDS             complement(16069..17193)
                     /locus_tag="PLH85_10690"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNB65077.1"
                     /translation="MQAHSVGIVAPQRARFTEALPLQSGGVLPEYDLVYETYGQLNAA
                     RSNAVLVCHALSGSHHVAGTYADQPGSEGWWDNLVGPGKPLDTNRFFVIGVNNLGGCY
                     GSSGPNALNPATGKPWGADFPFVTVEDWVNAQARLADRLGIERFAAVVGGSLGGMQAL
                     SWTLQYPGRVAHALVIASAPKLTAQNIAFNEVARQAILKDPDFHGGHYYEHGVVPARG
                     LALARMVGHITYLSDDAMGQKFGRTLRHDKPVFSYDVEFEIESYLRYQGDKFAGYFDA
                     NTYLLTTKALDYYDPAFAHQGNLVAALARAKADYLVVSFTTDWRFSPARSREIVYALV
                     HSDRRVSYAEIDCPAGHDSFLLDDPQYHAVLRAYIDNIRV"
     gene            complement(17366..19087)
                     /gene="ptsP"
                     /locus_tag="PLH85_10695"
     CDS             complement(17366..19087)
                     /gene="ptsP"
                     /locus_tag="PLH85_10695"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNB65078.1"
                     /translation="MSFTVHGLAVSGGIAIGHAHLVSHALLEVAHFTVAPRHVEAEVA
                     RLHGAVATVKADLAELKAATGAGAAPSEVDAFVGMHIMFLEDPMLVDAAEALIRTRRA
                     NAEWALVQQMEHLVEQFEAIEDAYLRERKADVVQVVERIVKVLLGHPGHLPPKRKDGL
                     GTIVVAHDLSPTDTIGFKDHAVAGFITDVGGPTGHTAIVARSLSIPAVVALRHIRHVV
                     KDDEMIIIDGTRGVVIVDPDERVLEEFRLRKVEFELERSKLKRLKSTRAATLDGEEVQ
                     LLANIELPPDAAQAKSVEADGVGLFRTEFLFMSRETWPDEDEQFEAYRSVVKTMSGKP
                     VTVRTLDVGADKELEGATRAEDNPALGLRAIRYCLSEPKMFLTQLRALLRASHYGRLK
                     ILIPMLASSHEIDQTLVLLEKAKEQLRAKKIKFDEAVPVGGMIEVPAAALCLGAFTRK
                     LQFLSIGTNDLIQYTLAIDRGNEAVAHLYNPLHPAVLRLLYQTIQAGAKAGVPVSVCG
                     EMAGDPSCARLLIGMGLRHFSMHPAQILEVKREILRADAADLTAKVQRLLKLDEPDRV
                     REAVERL"
     gene            complement(19146..19415)
                     /locus_tag="PLH85_10700"
     CDS             complement(19146..19415)
                     /locus_tag="PLH85_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNB65079.1"
                     /translation="MPRQEAEIINKLGLHARASAKLTQTASNYASEVWLERNGRRVNA
                     KSIMGVMMLAAAKGSTIVVETEGTDADQALSAVIALIADKFGEEE"
     gene            complement(19481..19864)
                     /locus_tag="PLH85_10705"
     CDS             complement(19481..19864)
                     /locus_tag="PLH85_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HNB65080.1"
                     /translation="MIGLLLITHGSLGEALIQCVSHVLNRRPPQLMQIGVTGQDDPTE
                     SLPLARRLLHMVDSGDGVLILTDVLGATPSNMATKLLEPGRIEGIAGVNLPMVLRCLT
                     YRERNMETLVKKAVAGACEGVVRIR"
     gene            complement(19948..21009)
                     /locus_tag="PLH85_10710"
     CDS             complement(19948..21009)
                     /locus_tag="PLH85_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNB65081.1"
                     /translation="MSPLRPGRRSLLRALLGLCALALLAGCQRERIQQQESYVFGTRV
                     EVLTWDADETRARDAVAEVLREFDRLHRAYHAWEPSELTALNAAIAAGKQAIPVSPEL
                     AAMLSDAQAIAATGDQLFDPALGQLIALWGFHTDTFVPHRPAPAALAAAVRAHPQMAD
                     LTIQNNTVSSRNPAVQLDLGGYGKGYALDRAADILRARGVRNALINIGGNVMALGSKG
                     EQPWRVGIQHPREPRPLASLPLRDGEAIGTSGDYQRFFELDGQRYCHLIDPRTGLPAR
                     GTQAVTILITPRAHAGTLSDAASKPIYLGGERWREYAARFGIDHALRVDADGHIQVTR
                     ALERRLQFSDGIKPSTVVD"
     gene            complement(21006..>21574)
                     /locus_tag="PLH85_10715"
     CDS             complement(21006..>21574)
                     /locus_tag="PLH85_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNB65082.1"
                     /translation="AITEFPQFIPPTRVARAAVDVHAFIDAHGDTILKPLDGMGGSQI
                     FRVRADDPNRNVIVETLTHGETRSIMAQAYLPAIVHGDKRVLIVGGRVIPYSLARIPK
                     DGETRGNLAAGGRGVAMPLTDGERAIAGHLAPILWARGLLIVGLDVIGDRLTEINVTS
                     PTCMVEITAQTGFDVPGAVIDALGAACA"
BASE COUNT         3143 a         6882 c         7990 g         3559 t
ORIGIN      
        1 gccgccgccg aattcctcgg caccaccctt gccgagcgcg gcatcgaact tgtttatggc
       61 ggcggcaacg tgggcctgat gggggtggtg gccgatgcct gcctcgctgc cggcgggcgc
      121 gtggtaggcg tgatcccccg cgccctgctt gactgggagg tgggccacga agggctcacc
      181 cgcctggagg tggtggactc catgcacacc cgcaaggcgc gcatggccga actggccgac
      241 ggcttcatcg ccctgcccgg cgggctcggc accttcgagg agctcttcga gattctcacc
      301 tgggcccagc tcggcttcca cgccaagccg gtcggactgc tcgacaccgc gggctactac
      361 ctgccgcttc tgcagatgat cgagcggggc gtggctgagg gattcatgaa ggccgagaac
      421 cgcggcctgc tgctcgccga agacaacctg ttcgccctgt tgcgggccat gggccaatac
      481 cacccgccca ccgtgtccaa acggatccgg gaggagaagc acctgtagcg accatcggcc
      541 gaccccgcgg cccccataaa aacacatcag ggaagaggag aaatgagcgt catccgatcc
      601 agcatcaacc cccgttcggc cgaattccag gccaacgccg ccgccctgcg caccctggtg
      661 gcggacctgc gcgacaccgt cggccgcgtc gccctgggcg gcggtgcagc ggcccgcgac
      721 aagcacaccg cccgcggcaa gctgctgccc cgcgaccggg tcgaccacct cctcgatccc
      781 ggcgccccgt tcctggagat cggccagctc gccgcctggg gcctgtacga caacgaggcg
      841 ccctgcgccg gcctcatcgc cggcatcggc cgggtgagtg gcgtggaatg catgatcctc
      901 gccaacgacg ccaccgtgaa gggcggcacc tactacccgc tgaccgtcaa gaagcacctg
      961 cgcgcccagg agatcgccga gcagaacggc ctgccctgcg tctatctggt ggattccggc
     1021 ggtgccttcc tgccccgcca ggacgaggtc ttcccggatc gcgaccactt cgggcggatc
     1081 ttctacaacc aggccaacct ctccgcccgc ggcatcccgc agatcgccgt ggtgatgggc
     1141 tcgtgcaccg ccggcggcgc ctacgtgccg gccatgagcg acgagaccgt catcgtgcgc
     1201 aaccagggca ccatcttcct gggggggccg cccctggtga aggccgccac cggcgaggag
     1261 gtgagcgccg aggacctggg cggcggcgac gtgcacacgc gcatctccgg ggtggccgac
     1321 cacctggccg agaacgacgc ccacgcgctg tacatcgccc gccgcatcgt ggccaacctg
     1381 aatcgggtca agccggtggc gctgcgcctc gccgacgccg aggagcccct ctacgacccc
     1441 gaggagatct acggcatcct gcccagcgac acgcgcaagc cctacgacgt gcgcgagatc
     1501 attgcccgcg tggtggacgg ctcccgcttt gacgaattca aggctcgcta cggtaccacc
     1561 ctggtgtgcg gcttcgccca cctctacggc tacccggtgg gcatcgtcgc caacaacggc
     1621 atcctcttcg gcgaatccgc ccagaagggc gcccacttca tcgagctgtg cgcccagcgc
     1681 ggcattcccc tggtcttcct gcagaacatc accggcttca tggtcggccg caagtacgag
     1741 aacggcggca tcgccaagga cggcgccaag atggtcaccg ccgtcgccac cgcccaggtg
     1801 cccaagttca ccgtgctcat cggcggcagc ttcggcgccg gcaactacgg catgtgcggc
     1861 cgtgcctaca gcccgcgctt cctgtggatg tggcccaaca gccgtatctc ggtgatgggc
     1921 ggcgagcagg ccgccagcgt cctcgccacc gtgcgccggg atggcatcga ggccaggggc
     1981 ggccactgga gccgcgacga ggaagaagcc ttcaagtcgc cgatccgcca gcagtacgag
     2041 gaccagggcc atccctacta cgccaccgcg cgcctgtggg acgacggcgt catcgacccc
     2101 gcccagaccc gccgcatcct cggcttgtcc ctgtcggcca gcctcaacac gccgatcctg
     2161 ccgaccaggt tcggcgtgtt ccggatgtga ggcgggcgcc atgacctacg agacccttga
     2221 aatcgaacgc gccggcgccg tcgccaccgt gtggatgaac cgccctcagg tgcacaacgc
     2281 cttcaacgag accctgatcg ccgaactcgg cggcgcctgg gccgccctgg acgcggacga
     2341 cgcggtgcgt gtggtggtat tggccgggcg cggacggagt ttttccgctg gcgccgatct
     2401 ggcctggatg cagcgtgccg gcgctgcgtc ggtggacgac aacctggccg acgccggccg
     2461 cctggcgggc atgctgcgcg gtctggcgga gctgtccaag cccacggtgg cgcgggtgca
     2521 cggcgcggcc ctgggtggcg ggatgggcct cgccgcggcc tgcgacctgt gcgtggcggg
     2581 ttccggggcg gtgttcgcca cctcggaggt gcgcctcggc ctgatcccgg cggccatcgg
     2641 gccctacgtg atccgcgccc tcggcgagcg ccaggcctac cgctacttcc tcacggccga
     2701 acgcatcgac gcgcggcggg cggcggcgct gggcctcgcc cacgaggcgg tggaggcgga
     2761 tgcgctggat acccgtgtcc gggacctgtg cgacgccctg ctgctcggcg ggccccaggc
     2821 ccaacgggcg gccaaggcgc tgatccgcgc cgtggcgaag cggccggtgg acgacgccct
     2881 ggtggccgac accgcccatc gcatcgccac cctgcgggcc acgccggaag cgcgggaagg
     2941 gctcgacgcc ttcctcgcca aacggccggc cgcctgggtg ccgggcgcct gaggtcgcga
     3001 tggactggac gccggcgggg ctcgcggtgg ctgcggaagg gctgcgtccc ctgctcgacg
     3061 ggggcggcat cccgctggac ctggcttccc tgacggcgct ggcggccggc ctgggctggg
     3121 cgagcggcct gcgcctgtac gcgctggtct tcgttctggg ggcgctgggg cgttttgccg
     3181 gggtgagcct gcccggcggt ctggcggtgc tgagtcatcc cctggtgctg ggactgtccg
     3241 gcctgctgct gctggtggag ttcggcgccg acaagctgcc ctggctcgat tccgtatggg
     3301 acgcggtgca taccttcatc cgcatcccgg cgggtgcggc ccttgctgca gcggtgatgg
     3361 gcgaccaggg cggtgctgcc caggtggcgc tgggcctgct cggcggcacg ctggcggcgg
     3421 gcacccattt cgccaaggcg ggcacgcggg cggccctcaa cacctcgccc gagccggtga
     3481 gcaatgtggc cgcgtcggtg ggcgaggacg ccctgttcgc cggaggcctg tggacgctgc
     3541 tgcatcaccc gctggtgttc ctggcggcgc tggcgctctt cgccgttgtc gccgggctgg
     3601 tgctgcgggc cctgtggggc gtcatgaaaa aggtgctgcg gccgcggatg cgcccggcgg
     3661 caccccgata acaaaaggac aactggagag gagacagcat gttcgacaag atcctgatcg
     3721 ccaaccgggg cgagatcgcc tgccgggtga tcaagaccgc ccgccgcctg ggcatccgga
     3781 cggtggcggt gtattcggaa gccgacgccg gtgcacgcca cgtgcgcctg gccgacgagg
     3841 cggtgctgat cggtcccgcc gccgcgcggg agagttatct ggtgatcgag cgcatcatcg
     3901 aggcggcgca gcggaccggc gcccaggcga tccacccggg ttacggcttc ctgtcggaga
     3961 acgaggcctt ctgcgaagcc tgcgaggcgg ccggcatcgt gttcatcggt ccgccggtgg
     4021 cggcgatccg cgccatgggc tccaagtccg aggccaagaa gctgatggag caggccggcg
     4081 tgcccctcac gccgggttac cacggcgacg accagacgcc ggctacgctg cagcgggagg
     4141 ctgacgccat cggctatccg gtgctcatca aggcggcggc cggtggcggc ggcaagggca
     4201 tgcggcgggt ggagtgcagc gccgacttcc tcgacgcgct ggcctcgtgc aagcgggagg
     4261 cggcgtccag ctttggtgac gaccacgtgc tggtggagaa gtacatcctg cgcccgcgcc
     4321 acatcgagat ccaggtgttc ggcgattcgc atggccacgc cgtgtatctc ttcgagcggg
     4381 actgctcggt gcagcgccgc caccagaagg tgctggagga agcgcccgcg ccgggcatga
     4441 cgccggagcg ccgcgccgcg atgggacggg cggcggtgga ggcggcccgc gcggtgggct
     4501 acgtgggcgc cggcacggtg gagttcatcg cccagcagga cggcagcttc tatttcatgg
     4561 agatgaacac ccgcctgcag gtggagcatc cggtcaccga gatgatcacc ggcctcgatc
     4621 tggtggaatg gcagctgcgc gtggcggccg gcgagcccct gcccctcgcc caggaacagc
     4681 tcgctctcaa cggccacgcc ctggaggcgc gcatctacgc cgaagatccg ggcaagggct
     4741 tcctccccgc caccggccga ctgctgcatc tggcgccgcc ggacgaaggt ctgcacgtgc
     4801 gcgtggatac cggcgtggag gagggcgacg agatcacgcc ccactacgac ccgatgatcg
     4861 ccaagctcat cgtgtgggac gtgaaccggg accgggccct ggcgcgcatg ctccaggccc
     4921 tggcggacta ccgggtggcg ggcgtcgcca acaacatcga gttcctgtcc cgtctcaccg
     4981 cctgcccggc ctttgccaat gcggacctgg acaccggcct catcgagcgg gagcaggcct
     5041 atctcttccc cgaagcgtcc gcgccggagc gggatgagtg gctggccctg gccctggccg
     5101 agctgttgcg ggaacaggaa cgggcggctg ccaccgcggc ccgcagcgcc gatccctcct
     5161 cgccgtggca cgcccgcgac ggctggcgca tgaacgcagc gccggcccgc agcctgatct
     5221 tccgccatgg cgaagcgcag aagaccctcc gcgtggcgac cctgcccggc gccttcgaga
     5281 tcaccctgga cggggtcgcg acgagggtgt ccggcagcct caatccgcgc ggactgctgc
     5341 gcggcgaatt tggcggccgg cgtctggcgg cgaccgtggt cgcggccggc gagcggcgtc
     5401 atgtgtttgc ccacgggcgc gcgcgggtgt tcgcctgcgt cgatcccctg ttcttcagcg
     5461 gggagggcgg cggcgcggag ggcgggctgc tggcgccgat gccgggcaag gtcatcgccc
     5521 tgctggcggc ggaaggggcg cgggtggaca agggcgcccc gctgctgatc ctggaggcga
     5581 tgaagatgga gcacaccatc gccgcgccga cggcgggcac cgtgaagggc ttccgcttcg
     5641 ccgtgggcga tcaggtggcg gacggcgccg aactggtgga gtttgacgca gcatgaaggg
     5701 acccgacatg actttgcccg cgcgggtgcg catcgtcgag gtgggccccc gcgacggcct
     5761 gcagaacgag gcccaggtga tcgccacgga caccaaggtg gccctgatcg accggctcgc
     5821 ccgggccggc ctgccggccg tggaggccac gtccttcgtg tcgccgaagt gggtgcccca
     5881 gatgggggac gcggccgagg tgctggcccg catcgaacgt ctccccggtg tggactaccc
     5941 ggtgctgaca cccaacctga agggcttcga cgcggctctg gcggccgggg cgaagtcggt
     6001 ggcggttttt gcggccgcgt cggaatcctt cagccggcgc aacatcaact gctcggtggc
     6061 ggaatccctg gcccgcttcg aaccgatcat ggcggccgcc gatgaggccg gcgtgcgggt
     6121 gcgcggctat gtctcctgcg tgctgggctg tccttatgag ggcgcggtgg ccccatcggc
     6181 agtggccgag gtggcgggca cgctgtacga catgggctgt tacgaggtga gcctgggcga
     6241 cacgatcggc accggtacgc ccggcgcggc ccgacggatg ctcgacgcgg tggcgcagcg
     6301 ggtgcccgtg gagcagctcg ccgggcatta ccacgatacc tatggacagg ccctggccaa
     6361 cgtctatgcc tcgctgcagg ccggtgtggc cgtgttcgat gcgtcggtgg gcggtctggg
     6421 cggctgtccc tacgctgcgg gcgcgtccgg caacctggcg accgaggatc tggtctatct
     6481 gctgaacggg ctgggcattg ccagcggcgt cgatctggag gcgctggtgg acatcgccgc
     6541 ctggatcagc gcccagctgg ggcgtccgcc cgcttcgcgc gtggcgcggg cgagcctcgc
     6601 ccaccgtggc cgccgcggct gtgcggcctg agcccatttc ctgccggccc atgtccgagc
     6661 ggtggccggc cctttctgac ggaaccccat gagcgtcgca tccccttgta ccggcatctg
     6721 ccgcatcgat ccaatgactg gctggtgtgc aggctgtcgc cgcaccctga ccgaaatcgc
     6781 cgagtggggc gccgcgggcg atggccgccg gcaggccatc ctggatgggc tggcgcagcg
     6841 tcgctggccg gaaggcgcgc cggccgacaa ggcatccgcg gacgtcgtgc cgccaccggc
     6901 agcgtccact ccatgaaaaa agggtgcccc ctcgtggagg cacccttcgt gcggcgttcg
     6961 acgggtcagg cggcccgacg gcggcgcagg gcgccggcca tggccacgcc gaggccgacg
     7021 agggccatgc tggtgggctc gggcacgcga ttgctgaggg tggtgtcgag cacgatgttg
     7081 tccacctgga tccagttgcc ctgggtgctc accggcgtcc acgaaaccga ggtgaggttg
     7141 gcgaggtcga aggtgaaggt gtgcaggcct gccaccgtgg gcaggttgac ggtgctgtgg
     7201 ctggtgctgt tgttgctgta caggaagtcg aacaccacgc tgccgccgga accgaagttg
     7261 taaacgtcgg tcaggtcgat ggagttgaag gtgaagctgc cgccgccggt cttgctcagc
     7321 gtgctggtcg tgccgccgta gttgtggctg taggtgttgc cgttggggtc ggcgttgtag
     7381 gacagggtgc cccagaacag catcgcggag ctgttggtgc tgctgttggt gatcgtgaag
     7441 ccgtcttcct gatagggcga gggcagggtc tggaagccga tgacctggtt gaagtcgatc
     7501 acggtggcgt gggcatgcag ggcgctcagg gagagcaggg cggcggcgag ggtcttgatc
     7561 ttcatggcgg acagctccag ataaatggga tggttggttc tgcatccagc gaaggctcac
     7621 gcacggtttt gaagattgtg gtgagttcgt tggttttctg gcgctattgt gaatttgggt
     7681 agaatccggg agcgcgcaga aaatcacgcg cccgtcgcgc cgaacgcggc ccggggcggc
     7741 tcattgcgtc cgtgcgccac ctgtgtcacg gtcagggggc gcgatgatcc gcgaggccgg
     7801 gccgggtatc gcgcgccggc gatgtcgccg aaagacccac gaggacagca tggacacgag
     7861 tgacgagaac ccctgcgtgg ggatttgcga gatcgacgag agcggctatt gcgtcggttg
     7921 cggacgcagc gccgacgaga tctacggtga cagcgaaccg gccccgtgtg cgacggacac
     7981 gctgccggcg gtcgaggcgc cgccgccgga cgcctgagcg ttccacgctc gttcacaaaa
     8041 tgcgcgtgcc gtcacggagc atgcgtatga ttgggcattg acccggcgta ccgcagcgta
     8101 cggttgcgcc ggcgcgagag gcttgtacag accgacgccg cccactcctc cagcccccac
     8161 atccatcgcc ccatgcaact cgtactcatc agtggcctgt ccggatcagg taagagcgtg
     8221 gccctcaacg tcatggaaga cagtggccac tatgtcgtgg acaacctgcc atcgacgctg
     8281 ttgccccagc tgatttccaa tctgcggggc ggcggctatc agcgcgtcgc cgtggccgtg
     8341 gacgtgcgct cgggcgccag tattgccgcg ttgccccagc aggtcgcgct gttgcgaggc
     8401 atggtcaccg acttgcgctt cctgtttctc gaagcccgcg acgagacgct catcgcgcgt
     8461 ttttccgaaa cccgccgcaa gcaccccctg ggggacgagt gctccaccct ggcagaggcc
     8521 atcggccgcg agcgggaggc gctggcgctg atctccgaac tgggccagcg catcgacacc
     8581 agcgacatct cggccaacac cctgcgcaac tgggtcaaat ccttcatgga cctggaggcc
     8641 agcagccaga tcaccctgct gttccagtcc ttcgggttca agcacggcat tccggtggac
     8701 gcggatctgg tgttcgatgt gcgctgcctg cccaatcccc actacaaccc ggagctgcgt
     8761 ccgctgacgg ggcttgacgc gccggtgatc gacttcctcg aacgggtgcc tgaggtcggc
     8821 cgcatggtgg aggacatcta cgccttcgtc gcgtcctggc tgccggccta tgtgcacgac
     8881 aaccgcagct acctcaccat cgccatcggc tgcaccggtg gccagcaccg ttcggtgtat
     8941 gtggccgagc agcttgccgg gcgcttccgc gagagcgcgc gggtgctggt gcggcaccgc
     9001 tccttccgcc gatgagccgc ctggccgacc tgcgcacgtg gctggcggcg tgggtcgcct
     9061 acctgcgtgt gggggacgtg ctggtgctgg ccctcggcct gggcgcgtcg gcggcctcgg
     9121 cgctggtcct gtggcagggc ggggcaccgg acaaggcggt catcaaggcg ggcgggcgcg
     9181 tgtttgccga ggtggacctg agcgcgccgc gcatcgtcga ggtgcccggc ccgatcggca
     9241 ccacgcgcat cgagatccag cctggccggg cgcgggtggc cgccgatccg gggccgcggc
     9301 agtactgtgt gcgccagggc tggctgcatt ccgccggcgc ggtggccatc tgcgccccca
     9361 atgaagtgag cctgtccctg tcgggccgta atgccgccta tgactccctc aactactgag
     9421 atccgcctgg tgcccaccgc ggacgaccac cgggtggccc gtcatgcggc ggcggccatc
     9481 gtcctgaccg tggcggaggc ggcgatcccg ctgccgttgc cgggcgtcaa gccggggctt
     9541 gccaacatca tcaccctggt ggtgctggcg cgctggggct ggcgcgatgc ggcctgggtg
     9601 gcaatcctgc gcatcctggc aggcagcctg ctgctcgggc agttccttgc gccgggcttc
     9661 ttcctcagtc tggccggcgg actggccagc ctggcggtgc tgggggcggc catgcatctg
     9721 ccgcggcgct ggttcggtcc ggtgacccag agcgtgctgg cggcctttgc ccatatcggc
     9781 gggcaagtgc tggtggcgcg cctgtggctg gtgccccacg acggggtgtt ctatctggta
     9841 ccggtttttg ccctggccgc gacggtattc gggctgggca atggcctcat tgccgcgcgc
     9901 ctgctggccg aggaagtgcc cgggcgttga ttcagcggcg tccgcgccgc ttgcgcgccg
     9961 aaaccggccg cggcgccggc ttgtcgggca ggcccagcgc cgcgcgactc agccggtcgg
    10021 cgtcgtggtt gcggtggcgg ggaatccagt gcaggtgcac cagggtgaag ccggtcagct
    10081 gacggccgat gacgtccagc aaggcgcgca gccgcgggat gtccgtgtgg gcggcgccgt
    10141 tcaggtggcg gacggcgaaa tcgctgtcgc cccagacctg cacatgggtg gcgcctgcgc
    10201 cacgggccag ctcaagcccc cggcccaggg cgagcagttc cgcttcgttg ttgcagccac
    10261 attccggcgc cagggcggaa taggcgtggc gtcggccgtc cggcgcaatg agcacgccgc
    10321 ccagtcccag gcgtcccggg ttggggtggg ctgcgccatc gaaccagagt tgccagagat
    10381 cccgggcatc gggccgaagg ggtgcgtcgc cttcaacaac cggtggcgag agcggttttt
    10441 gccgggaatt catcatttta tgtaaaacat ccctgaataa ccgtggttac ttcatggtta
    10501 ttgacgggcg tcaaggccga tccgtcaggg gcgtcaagaa tgaaatcagg ataaagccat
    10561 ttactgccaa acgggagtga aatcatggcg cattgtcctg aaatcatcac caccggtcag
    10621 tcgcaggggc gcgcgcgcat gcttgcgcgg gccttcgggc actggtttga tcttgacgcg
    10681 gctgctgcgg ccgcaacggg gttggacttg aaccgtgcgt ccgacaccgc aggcgggttg
    10741 cgggagcgca tcgtgcggac tcttgccggc cgagcaccgg gcggcgagcc gtgggcggcc
    10801 ctctttgcgg aagcgttgag cgaggccgac ccggatgccg cggccctggt cggggcggca
    10861 gcggtggacg gcatcgtcgc cgacggtcgt gcatgcgcgc agctgatgga gtgggaagcg
    10921 cgcctcgcgg ctctggccac gcggccctgc ggggggccgt cggcgcaggc tgcggcggcg
    10981 ctcgccctgc ggcgggggct cgtggctctg gtgctgcgcg gcgatgcgca ggccgcctgg
    11041 acgatctggg atgacggggt gcaccaggcc gagcgggccg cctcggaacc gctgcagctg
    11101 gcgctgacgg gcgcgcgggg cgtggccgag tgtctggcgg gagcggtcac ccgcgccgac
    11161 gtgcttgtca gtgacgcctg ggtgttgtgt gaggtcaagg cgcgcagccg cctgccctgt
    11221 ttactggtga cggtggccct gagcctgatc catctgatga tggaccggag tagcgacgcg
    11281 ctggcgctgc tgcagcgcac atgcgcagcg gccgccttcg aaggcctgcc cgaatcgctc
    11341 caggtgctgg tctggagtct gggcatgcac gcggcggccg acgtgggcga tacggatcgg
    11401 gtggccgact ggtcacgggc cgtcgccgac cggacggttc cggtgccgcg cctgctcctg
    11461 cacgcctgtc gccattatgt gctgggggcc atggcgctgc gccaaggcga cgccctgcgg
    11521 gcccgcatcc atgcggatgc ctgcatcggt gtcggtgagc gcgccggcgc cctgctgccc
    11581 gggtacatcg gccgcctgct gcgtctgcag gcgctggccg atctggcgga tgccggggcg
    11641 tccctgcgca ccgaagccgc gcagtggatc ccgacctggt cggcgtgcgg catggcgcgt
    11701 ctggcggccg tggcgcacct tgagttggct gcgcgggatg tgcgggccgg tgaactggat
    11761 cgggcgcggc gggcgtggtt cgcggcactg gcggctctac cgcccggtga atccctgcga
    11821 cccttgaacc gatcgggcgt ctggctggag gcgctgcgtg tccggctctt cccgatgggg
    11881 gaccggtgcg acgcgaccac tgcggcggtc cgcatccgga ccctcggtga attttcggtg
    11941 gagatcggcg ggcgccggat ctttgatcgg gactggaaag gcagccgcac gaagaccctg
    12001 ctgatggccc tcatctgcga aggcggcagc aaggtgtcgg tggaccgcct ggccgatctg
    12061 ctgtggccgg attcggatgg cgcccaggcg cgccagaacc tgaaagtggc cctgtggcgt
    12121 ctgcgccgtc tgggatgcga ggggccgccg gaggtgaatt gggtgcacgt gaagcagggg
    12181 cgggtcagtc tgccggcggg tctcgtgcag gtcgacgcgc tggaattccg caacgtggcc
    12241 cgcgccgcgt tgatgccggg ggcggatgtc ggtctgatga gggaggctct ggcggcccag
    12301 gctgaatcat tcctgcccat ggaggacaac ctcccctggg tggccgcatt tcgaaagggg
    12361 ctgatgggct tgaagcaggc cgtgcaggca cggatcgccg ggtgcgggga acaaggcgcc
    12421 gtacgggtcg tcggcgaagc ctgcacgagc gagttgaatg gcgcgtgacg aggggacgtc
    12481 cggtgttcgc ggcaacggcg ttgcgacctc tctgccggca ccgacgggcg tggcgcgggc
    12541 gatgccgtaa ccgatttgaa accgcggcct ggcgaagatg agcggaaccc ccttttcggt
    12601 gcgccgtgcc gggaggggcg tgaagacatc gcacagcggc gatgggccgc ccatgaggag
    12661 tcctggcgaa atgaccgata tcttgagagc aatgcgcatc gtcgtggcgt ccctgttggt
    12721 ctcggggctg gtcggtgccg ccaacgcggg gccgtatcag acctatccac tgagcgcgcg
    12781 gaccgactat ctgtggatgg cgactgacaa cgcgacgctt ggcgagaact ggatcagggg
    12841 cctggatggc aataatcccg ccgccgtcga tgcacagcac ccctatggaa actcggcgac
    12901 gaccgccctg gccgcagagg acatgatgtg gtactgcccg ccaggcgggt gtgcgtccaa
    12961 tacccggacg gggcttgcct atttttcgac ggtgttcaat ctgaactcgg atttcagggt
    13021 ggccgggggc cagctcaaaa tcattgccga cgattacttc gagctctatc tgaacggcac
    13081 cctgattaca ttcggcttgc tggacatgca catggacgcc agtggccagc ccgtgccgat
    13141 cattctcgac ctttcgctat acaaggatga tttttacggt ggtgagaatc tgatcgccat
    13201 caaggcgatg gatggctacc agatgtcgcc agggggcgtc tgttcgggaa cgaccatgca
    13261 gacgcaggtt ggcgatttct gtcagagcgc gcgcgggttt gaatacgttt atgtctccgg
    13321 cgccatcgac accattcccg aaccgcctgt cggatcgctc atggtctttt ccatggcggc
    13381 ccttgcggcg atgcggaggt ttggacgatc gtgagggtgt ccggcgacgc gcaggacggg
    13441 ttgcctggac aggcgtcacg ctgctgggtc aggtcgggct gaacgggcgg ccgccgccgc
    13501 cggcgccgct tggcacaccc gtcccgccat ccgcggtcgg cccaggcagg cgccccgtca
    13561 ccggatgagg cacagcttcc cgtcccgcac cggcggacac cagaaatagg ctccggtgag
    13621 ggggcgcgtg aagcggaaga gggcgtcgac gatgccgtct tcctggccgg tcatgcggcg
    13681 tagctgggct tcgaacggat cgagcgtggc gccgaaggcg acgaacatca ggccggcatc
    13741 gcgaccctcg ctccagggca tggagcgacg cagcaggaaa gcttcggggc tgaagctctc
    13801 ctgcgccgtg cgcttgacgt gggcggacgc gggcgcgtcg tccagttcct cgttgtcgct
    13861 gcgacggcgg ccgatgcagt cgtcgcgcgc ctcgggggcc atggccgcga ggcggcggaa
    13921 gtcgtgcagc cagcgctgta cggcgaccag gctggagccg tcggcggcaa tggcggcggt
    13981 cgtggcatcg tcaccctggg ggttctcggt tccgtcctca tagcccgaca gatcgcgtcc
    14041 gctgtcgtgc ttgaacgcgt cgatgctgtc cacgaggcgg aacaccggcg ccaggagcgc
    14101 ctcgatccgc tgcgcccgca gatgcagttc gccccgatcc tcaccccgca accacagcca
    14161 cagggccgcg ggcgtggccg gcagttcgat gccgggcgcg ctcggggtcg ggaaggcgtg
    14221 caggcccggc acgctgaggc cagcggccgt cacggtggcc agcccgaggc ccgccaccgt
    14281 gcgttcgcca tcgaccacgt caagcagggc ggacagcccg gcatggacgt cactgtccgg
    14341 ttcgagggtg aagcccaggt aacgggcgag gggcggaaca gggagcagga tgccggcttg
    14401 cggggtgttc atgggacggt ccgaaggggg aggaaacacg cggagatgat agtgcaatcc
    14461 cgtcggggcg tcaggcaggg ggcgagcggt cggcgcggcg ggcgcgccgt aaccggacgg
    14521 ttaaccatcc tggaaccact gcaggtgcag actggctgca gggcggcggg aatccctgcc
    14581 gcatgaacgg gaacgaggaa gagaggagca cgtgatgcag tcattcaatt ctccgcgcgc
    14641 cgtcctgacg gcgctgttga tgtcggccgg gctgggcact gcgtgggcag gaccggtggt
    14701 gtcgacgccc ttcgatctgg agatttcatc gacgcccttc gttacgggta cggcctacgc
    14761 gggggtgagc gtcaatacca cggcgagtgt cagcacgccc ctcgacgtga atggccgcgc
    14821 gtggacggat ctgcagtacg tggaggatcc gttggtgtgg acaggggtcc gctacaacta
    14881 tccggccccg tcgtccccgc agatcgcgac cgatccgacc cattgttcgt cgagccggcc
    14941 cggactgtgt tcgtacatct ggtatgaccc gaaccatacg tccagcggga cgaacggggt
    15001 cacccgggcg tatttccgca cgactttcaa cctgccggaa ttcaccggcg atgcggcgcc
    15061 cctgggggcg gtgctgaggg tccgcgccga tgatgatttt gccgtgtgga tcaacggctt
    15121 cctggtgatg ctgaatgccg attacggcac gtcgggcgac aaggggccgg attacgtgtt
    15181 cgaacggggc gtgagccaat atctgcggca gggtgagctc aacgtgatga ccttcgtcgc
    15241 caccgacggc agtctcaaca gcccgagcaa cgtgagctac gagcacctcg cctttgagct
    15301 gaagctgaac aaccaggtgc ccgaaccggc gagcctgatg ctgggcctga tgggcctcgg
    15361 cgggatcggt ctggcgcggc gccgccgcac cggctgaggc cggcggccgg ccttgcggac
    15421 acgacgcccc gtttgcgggg cgtcgtggcg taaatgggcc ggttctttgt cagtcaggcc
    15481 cgaccgagcc ggtaaagggc cacgctggcg agaaagttgg gttcttcgag gaccaccttg
    15541 tggtcgtcga aggccagccg ttcgtggatg gcgatgccct cgcgggcgca caggtcgtcg
    15601 aaatcggcga tggtgaagaa gcgcacgttg ggggtgttgt accactggtg cggcaggctg
    15661 tcggacaccg gcatgtggcc gttgatgatg gcctggcggt ggcgccagta ggcaaagttg
    15721 ggaaagctca ccaccgcctc gcggccgacc cgcagcatct cctgcaggat gcgctgggtg
    15781 ttgtgcatgg cctgcaggga caggctcatg atgacgtggt cgaagaagcc gttttcgaag
    15841 ccggcgaggc cttcttccag gtcgatctgg atcacgttga caccattgcg ggcgcaggcg
    15901 agcacccgtt cggggtcgtt ctcgacgccg tagccgcgga tgcggcgcac ctcgcccagg
    15961 tggcgcagca ggctgccgtc gccgcagccc aggtcgagaa cccgctcgcc gggttcgatc
    16021 cagttggcga tcacgtcgta gtcgtagcgc agactcatga tggtggcctc acacccggat
    16081 gttgtcgatg taggcccgca gcacggcgtg gtactggggg tcgtcgagga ggaaggagtc
    16141 gtggccggcg ggacagtcga tctcggcgta gctcacccgc cggtcgctgt gcaccagcgc
    16201 gtagacgatc tcccggctgc gggccgggga gaagcgccag tcggtggtga aggacacgac
    16261 caggtagtcg gccttcgccc gcgccagggc cgccaccaga ttgccctggt gggcgaaggc
    16321 cgggtcgtag tagtcgaggg ccttggtggt gagcaggtag gtattggcgt cgaaatagcc
    16381 ggcgaacttg tcgccctggt agcgcaggta ggactcgatc tcgaattcca cgtcgtagct
    16441 gaacaccggc ttgtcatggc gcagggtgcg gccgaatttc tggcccatgg cgtcgtccga
    16501 caggtaggtg atgtgaccca ccatgcgcgc cagggcgagt ccgcgggcgg gcaccacgcc
    16561 gtgctcgtag tagtggccgc cgtggaagtc cgggtccttg aggatggcct gacgggcgac
    16621 ctcgttgaag gcgatgttct gggcggtgag cttgggggcg gaggcgatca ccagggcgtg
    16681 ggcgacccgc ccggggtatt gcagggtcca cgacagggcc tgcatgccgc ccaggctgcc
    16741 gcccaccacc gcggcgaagc gctcgatgcc gaggcggtcg gccaggcgcg cctgggcgtt
    16801 gacccagtcc tccacggtaa cgaaggggaa atcggcgccc cagggcttgc cggtggccgg
    16861 gttgagggcg ttggggccgg aactgccgta gcagccgccc aggttgttga cgccgatgac
    16921 gaagaagcgg ttcgtgtcga ggggcttgcc cggcccgacc aggttgtccc accagccttc
    16981 ggagccgggc tggtcggcat aggtgccggc cacgtggtgg gagccggaca gggcgtgaca
    17041 gaccagcacg gcgttgctgc gcgcggcgtt gagctgaccg taggtctcgt agaccaggtc
    17101 gtattccggc agcacgccgc cgctctggag ggggagggct tcggtgaagc gggcgcgctg
    17161 gggcgccacg attccgacgg agtgtgcttg catccttgtt gtcccgaaaa acagaaaccc
    17221 agccggcgaa cggtcgcgga ctgggctgcc gtcgctttag ccgtacttgt aaagcgcccg
    17281 caagctgaag ggttcaaatc ggcgctggat cgaagagtag cgcgtcgtcg gcggccgcgt
    17341 caattgctgc gcccgccgac gccgttcaca agcgctccac cgcttcgcgc acccggtctg
    17401 gttcgtcgag cttgagcagg cgctgcacct tggcggtcag gtcggccgcg tcggcgcgca
    17461 ggatctcgcg cttgacctcc aggatctggg ccggatgcat cgagaagtgg cgcaggccca
    17521 tgccgatgag caggcgcgcg caactgggat cacccgccat ttcgccacac accgaaaccg
    17581 gcacgccggc cttggcaccg gcctggatgg tctggtagag cagacgcagc accgccggat
    17641 ggagcgggtt gtagaggtgg gcgacggcct cgttgccccg gtcgatggcc agggtgtact
    17701 ggatcaggtc gttggtgccg atggacagga actgcagctt gcgggtaaag gcgccgaggc
    17761 acagggccgc cgcgggcacc tcgatcatgc cgccgaccgg gaccgcttcg tcgaacttga
    17821 tcttcttggc gcgcagctgc tccttggcct tttcgaggag gaccagggtc tggtcgatct
    17881 cgtggctgct ggccagcatg gggatcagga tcttgagcct gccgtagtgg ctggcccgga
    17941 gcagtgcccg cagttgggtg aggaacatct tgggctcgga caggcagtag cggatcgccc
    18001 gcaggcccag ggccgggttg tcctcggcgc gggtggcgcc ttccagttcc ttgtcggcgc
    18061 ccacgtccag ggtgcgtacc gtgaccggct tgccggacat ggtcttgacc acgctgcggt
    18121 aggcctcgaa ctgctcgtcc tcgtcgggcc aggtttcccg gctcatgaac aggaattcgg
    18181 tgcggaacag gccgacgccg tccgcttcga cgctcttggc ctgggcggca tcgggcggca
    18241 gctcgatgtt ggcgaggagc tgcacctctt ctccgtccag ggtggcggcg cgggtggact
    18301 tgaggcgctt gagcttggag cgttcgagtt cgaattccac cttgcgcagg cggaactctt
    18361 cgagcacgcg ctcgtccgga tcaacgatga cgaccccgcg ggtgccgtcg atgatgatca
    18421 tctcgtcgtc cttgaccacg tggcggatgt ggcgcagcgc gaccacggcc gggatggaca
    18481 ggctgcgggc gacgatggca gtgtgcccgg tgggtccgcc cacgtcggtg atgaaaccgg
    18541 cgacggcgtg gtccttgaag ccgatggtgt cggtgggcga caggtcgtgg gcgacgacga
    18601 tggtgccgag gccgtccttg cgcttgggcg gcaggtggcc cggatggccg aggagcacct
    18661 tgacgatgcg ttccaccacc tgcaccacgt cggccttacg ctcccgcagg taggcgtcct
    18721 cgatggcctc gaactgttcg accagatgct ccatctgctg caccagggcc cactcggcgt
    18781 tggcgcggcg ggtgcggatg agcgcctccg ccgcgtcgac gagcatgggg tcttcgagga
    18841 acatgatgtg catgccgacg aaggcatcca cctcgctggg ggcagcgccc gcaccggtgg
    18901 cggccttgag ctcggccagg tcggccttga cggtggcgac ggcgccatgc aggcgcgcga
    18961 cttccgcctc cacgtggcgc ggtgccacgg tgaagtgggc gacctccagc agggcgtggg
    19021 agaccaggtg ggcgtggccg atggcgatgc cgcccgagac ggcgaggccg tggacggtga
    19081 agctcatggg cggtcctccg gagcggggtg ccggggagcg gtgccggcgg cgaggccggc
    19141 ggcgttcatt cctcttcccc gaacttgtcg gcgatcaggg cgatcaccgc gctgagggcc
    19201 tggtctgcgt ccgtgccttc ggtttccacg acgatggtgg agcccttggc ggcggccagc
    19261 atcatgacgc ccatgatgct cttggcgttg acgcgacggc cgttgcgttc gagccagact
    19321 tcgctggcgt agttgctggc ggtctgggtg agcttggcag aggcgcgggc atggagcccg
    19381 agcttgttga tgatttcggc ttcctggcgt ggcatggagg gtgttctccc gtttcctcga
    19441 agtgtggtgt acggattgtt gttattgacg tgatgacggc ctatcggatg cgcacgacgc
    19501 cttcgcaggc gccggccacc gctttcttga ccagggtttc catgttgcgt tcccggtagg
    19561 tgaggcagcg cagcaccatg ggcaggttga cccccgcgat gccttcgatg cggcccggtt
    19621 ccaacagttt ggtggccatg ttggaggggg tggcgccgag gacgtcggtg aggatcagca
    19681 ccccgtcgcc gctgtccacc atgtggagca ggcggcgggc caggggcagg gattcggtcg
    19741 ggtcgtcctg gccagtgacg ccgatctgca tcaactgggg cggtcgccgg ttgaggacat
    19801 gactgacgca ctggatcagg gcctcgccga gactgccgtg ggtgatgagc agaaggccga
    19861 tcatgtcccg attctatccg aaggccggag cggggcaagc cgggaatgta agggggaatg
    19921 agcccgtctt ttcggaccga cccggtgtca gtccacgacc gtcgagggct tgatgccgtc
    19981 cgagaactgc aggcgccgtt cgagggcgcg ggtgacctgg atgtggccgt cggcatccac
    20041 ccgcagggcg tggtcgatgc cgaagcgggc ggcgtattcg cgccagcgct cgccgcccag
    20101 gtagatgggc ttgctggcgg cgtcggagag ggtgccggcg tgggcgcgcg gagtgatcag
    20161 gatggtcacc gcctgggtgc cgcgggcggg caggccggtg cgcgggtcga tgagatggca
    20221 gtaacgctgg ccgtccagct cgaaaaaacg ctggtagtcg cccgaggtgc cgatggcctc
    20281 gccgtcgcgc aggggcaggg aggcgagggg gcgcggttca cgggggtgct ggatgccgac
    20341 ccgccagggt tgctcgcctt tgctgccgag ggccatgacg ttgccgccga tgttgatgag
    20401 ggcgttgcgg acaccgcggg cgcgcaggat gtcggcggcc cggtcgaggg cgtaaccctt
    20461 gccgtagccg cccaggtcga gctgcacggc cgggttgcgg ctgctcaccg tgttgttctg
    20521 gatcgtcagg tcggccattt gcgggtgggc cctgactgcg gcggccaggg ctgccggagc
    20581 gggccggtgc ggcacgaagg tgtcggtgtg gaaaccccac agggcgatga gctggccgag
    20641 ggccggatcg aagagctggt cgccggtggc ggcgatggcc tgggcgtcgc tgagcatcgc
    20701 ggcgagttcc ggcgacaccg ggatggcctg cttgccagcg gcgatggcag cgttgagggc
    20761 agtgagctcg gagggttccc aggcgtggta ggcccggtgc aggcggtcga attcgcgcag
    20821 cacctcggcc accgcgtcgc gggcgcgggt ctcgtcggcg tcccaggtaa ggacttcgac
    20881 gcgggtgccg aagacgtagg attcctgctg ctggatgcgt tcccgctggc agccggcgag
    20941 cagcgccagg gcgcacaggc cgaggagtgc gcgcaggagg ctgcgccggc cgggccgcag
    21001 ggggctcatg cgcaggcggc tcccagggcg tcgatgacgg cgccgggcac gtcgaagccg
    21061 gtctgggcgg tgatctccac catgcaggtg gggctggtga cgttgatctc ggtgaggcgg
    21121 tcgccgatca cgtccaggcc gacgatgagc aggccgcggg cccacaggat cggcgccagg
    21181 tgcccggcga tggcccgctc gccgtcggtg aggggcatgg ccacgccgcg gccgccggcg
    21241 gcgaggttgc cgcgggtctc gccgtccttg gggatgcggg cgaggctgta ggggatgacc
    21301 cggccgccga cgatcagcac gcgcttgtcg ccgtggacga tggccggcag gtaggcctgg
    21361 gccatgatgc tgcgggtctc gccgtgggtg agggtctcga cgatgacgtt gcggttgggg
    21421 tcgtcggcgc ggacgcgaaa gatctggctg ccgcccatgc cgtcgagggg cttgaggatg
    21481 gtgtcgccgt gggcgtcgat gaaggcgtgc acgtctaccg cggcgcgggc cacgcgggtg
    21541 ggcgggatga actggggaaa ctcggtgatg gcga
//
LOCUS       DAOQMM010000060        21278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_865_length_21278_cov_48.117278, whole genome shotgun sequence.
ACCESSION   DAOQMM010000060 DAOQMM010000000
VERSION     DAOQMM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21278
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_865_length_21278_cov_48.117278"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLH85_10720"
     CDS             <1..495
                     /locus_tag="PLH85_10720"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNB65083.1"
                     /translation="AIAIGTSHGAYKFTRKPTGDILAIERIKAINARLPNTHLVMHGS
                     SSVPQELLEIIRQYGGDMKETYGVPVEEIVQGIKYGVRKINIDTDIRLAMTGAIRKFF
                     VENPSKFDPREFNKPAREAAKLVCKARFEAFGAAGNASKIKPVSLDKIAARYKSGELN
                     QIVR"
     gene            574..948
                     /locus_tag="PLH85_10725"
     CDS             574..948
                     /locus_tag="PLH85_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65084.1"
                     /translation="MAVPWSTLFGAIPWSDVIARAPDIAQGARKLWQKAGRRGDPPPD
                     ASPVAPSLDVRVDALAARLGDIAARQQDSAELLAALATQQAELIRATDALHRRARLLG
                     IACAILGLALAGLATAILVRAA"
     gene            1074..2375
                     /locus_tag="PLH85_10730"
     CDS             1074..2375
                     /locus_tag="PLH85_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNB65085.1"
                     /translation="MTPTTLFAEHDLRQTLNDEFHARPPVPLESPQLISYLAFHQQDV
                     AAGEAQRHLAELGERLGRPLDAGGPHCLLDFGAFQLKWELHNEFTSYTFIRPVARGEG
                     LDGSTSALAVVPQDWLRAIPGRLMVATHVELRSAAEVSPEEVMAQLSPTGRQMVGAMV
                     ADGAAWVFTDFLFEDGFSRFLVIDVSLTRRQAGRTVQRLVEIETYRLMALLAFPVAKD
                     VGRLLGRAESDLARLMDRTGSARSPEDERGVLADLSALAAEVEHSVARTTFRFGAARA
                     YHSLVMQRIEELRERRITGLPTFHEFMERRLLPAMNTCQTMARRQEDLSGRVARNSQL
                     LRTRVEVELERQNQELLAQMNRRAKLQLHLQETVEGLSVVAITYYASQLVQYMAKGVK
                     HFIEPLTPEGLTAAAIPVIAGIVALGLHRMRKALAAADGGH"
     gene            2459..3379
                     /locus_tag="PLH85_10735"
     CDS             2459..3379
                     /locus_tag="PLH85_10735"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNB65086.1"
                     /translation="MTTPLFESSLRSLPLLGRGKVRDIYAVDADRLLIVTSDRLSAFD
                     VILPDPIPRKGEVLTAMANFWFARLGHIIPNQLTGVDPESVVAADERDQVRGRALVVK
                     RLRPLPIEAVVRGYVIGSGWKDYQDTGAICGIALPAGLKQAQKLPAPIFTPASKADVG
                     DHDENISFEQAQARTAAQFADSGLDGARLVSEARDAALALYSEASAYAAGRGILIADT
                     KFEFGVDAAGTLHLIDEALTPDSSRFWPADSYAEGISPPSFDKQYVRDYLETLDWGKK
                     APGPTLPADVIARTSAKYVEAYERLTGLSL"
     gene            complement(3422..4291)
                     /locus_tag="PLH85_10740"
     CDS             complement(3422..4291)
                     /locus_tag="PLH85_10740"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB65087.1"
                     /translation="MDESELSLVEQAFLHTITMELERGELKFPTAMEVTLRVMEVLDD
                     PDADIARIARMVSAEPLLAARTVALANSAWYNPGGRLVADVPRALARIGIAKLRALAL
                     TVAAEQLATKDMLGPFQPQMQVLWAHCVDVAATACVLARHCSRINPDEAFFAGMVHDI
                     GQFFLLARVWEYPELLREGSPLSDLVRVWHPPIGRAVLASLHMPQDLVDAVDDPELYG
                     GRWPPENVADLIFIANLLAETGNPFSLEPEPFRNGLAKVATLGLDDVEIGAVLKESAE
                     EREALIACFSIHL"
     gene            4379..6448
                     /locus_tag="PLH85_10745"
     CDS             4379..6448
                     /locus_tag="PLH85_10745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNB65088.1"
                     /translation="MTTATAPSPLLQPTGLPRFDAITPDQVEPGLRSLLDAYGATLAR
                     LQDDPTTPTWANFVEPIELEGERLGRAWGVVVHMHSVMDTPEWREAYNAMLPEVTRFY
                     AELGQNLKLFEKYKAIAEGPEFAGLTPARQRILDNELRGFRLSGAELPDARKPRFKEI
                     QEELAGLSARFSENLLDATNAFSHLVTDPARLAGLPDDVVTAAREAAAKAGQEGWKFT
                     LKMPSYLPVMQYADDRALRETMYRANGTRAAEIDNGFSKPEWNNGPLIERILALRAEE
                     AAMLGYRTYADVSLVPKMADSPEQVLGFLRELAAKARPFAERDLAELATFARDELGLD
                     PLEPWDTGYASEKLRVARYDFSEQEVKQYLPEPKVLQGLFDVVEKLYGVRILPDSGPV
                     WHPDVRFFRIERQGALVGQFYLDLYARETKKGGAWMDSAISRRRLGDGIETPVAYLVC
                     NFPGPVGGKAATFSHDDVITLFHEAGHGLHHLLTRVDDLAVSGIHGVEWDAVELPSQF
                     MENFCWEWDVVREMTAHADTGEALPRALFDKMLAAKNFQSGMQTVRQLEFALFDLRLH
                     LDFNPANPRVMDLLDEVRQEVAVLVPPAWHRFPNSFSHIFAGGYAAGYYSYKWAEVLS
                     ADAFAAFEEAGRPQGSVLDAVTGARFWEEILAVGGSRPALESFKAFRGREPQVDALLR
                     HSGMVPA"
     gene            6445..6912
                     /locus_tag="PLH85_10750"
     CDS             6445..6912
                     /locus_tag="PLH85_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNB65089.1"
                     /translation="MKRRSALLLLIAAAPALAQTAYRWVDDKGRVQYSDQPPPAAVPQ
                     YDQRNISANQSGSLQQSFATRQAAGKYPVTLYVSKDCGKACEDGRAFLKKRTIPFSEQ
                     RIESAEDVATYKARIGAEPFVPTLLVGREKETGFAAPAWDNLLDKAGYPAARR"
     gene            7097..9184
                     /locus_tag="PLH85_10755"
     CDS             7097..9184
                     /locus_tag="PLH85_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65090.1"
                     /translation="MKTRNRLIAEALGGALFLSACGGGGGGSSTPASPQTPASVDVTL
                     TPSLGKFSTGCSVELRTGSGTLLGSAPITDAGNAFIRVTGYSGPVIAQVKGASGCTYF
                     DEATASQVPFGADRSLSAVSDTLRVDQGINVLTHLAASRVLDGDKLATGKTADTVKLE
                     NLTIQRMFQVADMFAAPTLLGNASDRLDNTAAGQLAVQLAALSELAHTRATDVATLAA
                     TLATDLADGTLDTFGASEYQVAAQTTVAKLASPSAQASLLILSQTPSLIVSTRDVQSD
                     VQKVIAAGPALAQAKQLFADLRTSVLALSNSAGTGTLDAENALLKADFQHGLGVDMLI
                     DGVKFMLESASQFFDSGTSRPGSTHSGWVGTCYQDSTTQATCHVSGVDDGSVYTSYQV
                     RLTKTGAGTASWAFTQVYRYQNGQSTQTAAPSGMSGSFTYSANAATAKGQFIPMTGDA
                     ERSAIDISLGFTTTGTTGAIRFSATGSANSVKTDGTSTLQVDLTKLDLDPVSQVNQMV
                     LSLTGPRHRFDGALTLSGQVASADGSIAQPKSLKFVGTFTDTASHYTVLDGTVTASQD
                     WTGYDPRLANSDGNFARYAFSFDGVANKGPGAAGITLSFSGDNQAGPHDQKIALKFTS
                     ASGIVVTGTGVQTPARPDDLWNWTFANENGIKATYAQSSRSGKVTAGDGSDLGTLSSQ
                     RVTFVDGSFESLM"
     gene            9260..10426
                     /locus_tag="PLH85_10760"
     CDS             9260..10426
                     /locus_tag="PLH85_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65091.1"
                     /translation="MRTSPVPVLIILAALPGLARADAADLLALVQPTRNDAATAVRVD
                     ALGFAADDFTPAHSIARTWNAPPTPRDGPNRALGFARLETSASHGTWSVAVFRRYDAL
                     GSGTPDTVRFYHALAHPDDLLARDQRLAIDYRFTGFSADGLRLTRHFSPAPDQARYAV
                     SLNLLKAGALRMERATGIATTAGGVATVNGRRSLLYTGLDPTPTSQAHLNDFAPVTTG
                     TPSAGWGVSVDLDARWQPRPDLHLSVATNDLLGRLHWQDVPEMVQDFNGTAWPLQFNQ
                     PQATARITGANHYRDVTLHLKPKVAAAAEWSVSPRWQADASLATVQGVVLPEAGLSWQ
                     ATAGPRVRLSYETRFGSAGVGVRMGPFYAEVRSDRFNLNDAHSLGARAGLTVAF"
     gene            10866..11624
                     /gene="xth"
                     /locus_tag="PLH85_10765"
     CDS             10866..11624
                     /gene="xth"
                     /locus_tag="PLH85_10765"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB65092.1"
                     /translation="MKIATWNVNSLKVRLPHVLDWLAAAQPDALCLQELKCENKAFPL
                     SEIEAAGYHAVFNGQKTYNGVAILSRTPAAEVSLDIPGFADEQKRIIAATVEGVRVVC
                     GYFPNGQAVGSDKFAYKLAWLAALTDWLRGELKTSPRLVLAGDYNIAPEDRDAHPDWK
                     DEIHVSAPEREAFRTLLDLGLSDAFRLFEQADKSFSWWDYRMGAFRRNFGLRIDHLLV
                     SPALKDVCRACYVDKAPRKLERPSDHAPVVVEFA"
     gene            11621..12283
                     /locus_tag="PLH85_10770"
     CDS             11621..12283
                     /locus_tag="PLH85_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB65093.1"
                     /translation="MSTLTPGDELAALYPVLAGLPDAARRRLGNHARRITVPAGTALF
                     DERQACQGFPFVLAGSIRVIKAAPSGRELPLYRVAPGETCIISSACLLGHAAYNARGV
                     TETDTTLLLLPDTDFQSLLAEPAFRDFVFQLFSERIADLMQLIEEVAFRKLDQRLAAL
                     LLGKGQRLHTTHQQLADELGSVREIVSRLLKGFAEQGLVALSREQIEIRDAAGLRRVA
                     AG"
     gene            12504..12695
                     /locus_tag="PLH85_10775"
     CDS             12504..12695
                     /locus_tag="PLH85_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNB65094.1"
                     /translation="MKSNVGGIDKILRIGGGVALVAWAALGGPAWAWIGVVPLATGLV
                     GFCPLYPLIGLSTCPMKKD"
     gene            12825..13418
                     /locus_tag="PLH85_10780"
     CDS             12825..13418
                     /locus_tag="PLH85_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNB65095.1"
                     /translation="MDSASSFRFVAVMTKIGLFFGTETGTTRLVAKRLHKQLGDDIAD
                     KPVNVNRIEPAELLRYDALILGTPSYGEGQIPGAGAGCFEPNWEEFLAKMPADADLRG
                     KRVALFGLGAQERYSERFCSSLFALYQVFKGYGAEIVGAWSTGGYTFQHSAAVVDGQF
                     VGLVIDQRTQGMFTDDRIAQWVAQVKPLLLEKLTAAA"
     gene            complement(13464..14261)
                     /gene="rsmA"
                     /locus_tag="PLH85_10785"
     CDS             complement(13464..14261)
                     /gene="rsmA"
                     /locus_tag="PLH85_10785"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNB65096.1"
                     /translation="MNAPHLARKRFGQNFLSDPNIIRKIIEAIRPAPDDRMVEIGPGL
                     GAMTEPLMARLDHLHVVEIDRDLIARLKAAHAPDTLTIHEGDALKFDFAALAADGRPL
                     RVVGNLPYNISTPLLFHLADSAERVKDMTFMLQKEVVMRMVAAPGTEDYGRLSVMLQY
                     RFAMARLFDVPPGAFRPAPKVMSSIVRMVPRPLAECTAIDEALLGRIVTAAFGQRRKT
                     LRNTLRDILSDPDFVALGLDPGLRGEALDVAAFVRIANHVAARDAQI"
     gene            complement(14258..15178)
                     /locus_tag="PLH85_10790"
     CDS             complement(14258..15178)
                     /locus_tag="PLH85_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB65097.1"
                     /translation="MRRRALLQAGAALLAASGLSACGGLGTRPDATAKVPAPGLPATP
                     RPRARIGLALGGGAARGFAHVGVIKALETSGIQPDLVVGTSAGAVVGSLYAAGHGPFE
                     LQKLAIQLDESAVTDWSFFDRGFIKGEALERFINTQAGNKPIEGLRRRFAAVATDLQS
                     GEVIVFQRGNTGIAVRASAAIPGVFPPVAVNGREYVDGGLVAPIPVREARSLGADLVI
                     AVDISGRPSGKKNQGSVDVLLDTISIMGGALGKVELAAADVVIRPNLQGLPATNFQQR
                     HEAILEGERAGFAAVPRIREALAAWERKAG"
     gene            complement(15175..16161)
                     /gene="pdxA"
                     /locus_tag="PLH85_10795"
     CDS             complement(15175..16161)
                     /gene="pdxA"
                     /locus_tag="PLH85_10795"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNB65098.1"
                     /translation="MSDSLPVLAITSGDPAGVGPDLCVALPSRPWAARPVVLGDHELL
                     AARAQMLAPDLAVVPYDPSAAPRDGTLEVLHVPLAVPAEAGRLDAANGRYVLDILDRA
                     IAGCRSGEFAGMVTAPVHKGIICEAGVPFSGHTEYLAEATGTPLVVMMLVGGGLRVAL
                     VTTHLPLKDVPAAITQPLLAEVLRILHADLRERFGLAAPRILVAGLNPHAGEGGHMGR
                     EEIEVIEPVLDALRAEGMDLVGPLPADTLFVPHTLARGDAVLAMYHDQGLPVLKHASF
                     GGGVNVTLGLPIVRTSVDHGTALDLAGTGRADPGSLFAAVELAISLSRGGRP"
     gene            complement(16268..17560)
                     /locus_tag="PLH85_10800"
     CDS             complement(16268..17560)
                     /locus_tag="PLH85_10800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB65099.1"
                     /translation="MRLLALLATLVFTHAAWAAGPVEVDRIVAVVNSDVVTRSDLRLK
                     VEQISRQLTRQGTPLPADDVLERQVLERLIMEKLQIQLANETSLRVDDLALDRALGRI
                     ADNNKLSMTEFRQALEKDGITWDRFREEIRTEMLLSRLREREVESRIVVSDAEVSNFL
                     AHPENTQSQEEFNLAHILLRAPEGATPEQLARLRARAEDVAARVARGEPFDKLAASYS
                     DAPDALSGGNLGWRSAERLPGLFADAVTRMKPGETSPILRSAAGFHIVRLIDRRGGGD
                     ALGATQVQQTHARHILIKTSEVVSDGDARRRLLDLRERIVQGGASFAELARAHSADLS
                     ASKGGDLGWIYPGDTVPEFEQAMDALKPGEISQPVQSPFGWHLIQVEERRVQDVSDER
                     KRNAARNALRERKLDEAYQDWLRQLRDRAYVDIRLEDK"
     gene            complement(17580..20111)
                     /locus_tag="PLH85_10805"
     CDS             complement(17580..20111)
                     /locus_tag="PLH85_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNB65100.1"
                     /translation="MKPRFQLRFLSVALFALGGRAGAESLPPFEVPAALLGKPEAIRS
                     RKAAPVPEPAVAAPPVIAAPSARSEAPGAASAAAAVVTAPVPAAGPSAPPQPVAAAVA
                     TRSGPAPAPVKNGESVITADKVFGRQDFDVTAEGRAVLLRDGTRVEGDRLQYFELTDE
                     VEATGNVRMTRGDDVMTGPHARFKVQEQVGTFDTPSYEIFRPPRPPRPGQQFDTTQQG
                     RRQPISGHGSAESLELEGENQFRFLNGTWSSCKPDRPDWYLRARELQLDFDREVGEAN
                     NGTLYFKDVPVVYLPWADFPLGDQRQSGLLAPSFGTSNKVGFDLAVPYYWNIAPNYDS
                     TITPRLMGRRGLQIRDEVRYMTPTAKGMSFVEWMPNDQVENRSRLAGQIRHNETTPTG
                     WVTAVDVSGVSDPRYFIDLSSRLSLTSQVNLLRQGSVGYYGGGWWNATGMLQAWQTLQ
                     DPNGAAVSVPYKRLPQLTLTANRPELPGGTAFLFNSEFVAFSHPTQDEGRRLTLYPQL
                     SLPLVTSAFYVTPKVGVHATEYNLDRRTSTGPDSIQRSLPILSVDSGVNFERDTQIAG
                     REFTQTLEPRIYYLYVPYRDQSNIPNFDSGVYDFNFAQIFAENYYTGGDRISNANQIT
                     TAVQSRLINPNTGAEVIRAAFGQRYYLEEQRVTLPGVAARTGGQADFLAAFGGQIAPR
                     TQLDTGWQYNPRDHWTERFNAGVRYQPGFARALNASWRYRRNYSTVAATPDGFRDLDL
                     TGQWPLAGNWYAVGRYNRNLRDHRLTEGIAGFEYNGGCWVARTVVQHLTTSATDTTRA
                     FFVQIEFSGLASLGSNPINMLRRSVPGYGKISDPGSSPFFTNDDY"
     gene            20204..21202
                     /locus_tag="PLH85_10810"
     CDS             20204..21202
                     /locus_tag="PLH85_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNB65101.1"
                     /translation="MERRQQLQSWLAAEFPGRAFDLAPASADASFRRYFRVTFADDPQ
                     PVIVMDAPPSHEDCRPYLRVAELFGGAGAHVPTVHKQNLEEGFLLLSDLGATTYLDAL
                     TADNAAHLYADALGALICIQHASRPGVLPDYDRALLLREMELFPVWYVGRHKGVTLSD
                     EQSQALYDVFDRIADVNLAEPKVFVHRDFHSRNLMVSTPNPGVLDFQDAVYGPLSYDL
                     VSLFKDAYIHWEEDFVLDMLARYWETARDLGLPVRADFAEFHRDFEWMGVQRHIKVLG
                     IFARLYHRDGKDGYLKDMPLVMHYLRKACERYGELRPLLRLLEQIDPLVTETGYSF"
     gene            21206..>21278
                     /locus_tag="PLH85_10815"
     CDS             21206..>21278
                     /locus_tag="PLH85_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003099549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB65102.1"
                     /translation="MKAMILAAGRGERMRPLTDTCPKP"
BASE COUNT         3447 a         7914 c         6727 g         3190 t
ORIGIN      
        1 gccatcgcca tcggcaccag ccacggcgcc tacaagttca cccgcaagcc caccggcgac
       61 atcctcgcca tcgagcgcat caaggccatc aacgcccgcc tccccaacac ccacctggtg
      121 atgcacggct cctcctcggt gccccaggag ctgctggaga tcatccgtca gtacggcggc
      181 gacatgaagg aaacctacgg cgtgcccgtc gaggagatcg tccagggcat caagtacggc
      241 gtgcgcaaga tcaacatcga caccgacatc cgcctcgcca tgaccggcgc gatccgcaag
      301 ttcttcgtcg agaacccctc caagttcgac ccgcgggagt tcaacaagcc cgcccgtgag
      361 gccgccaagc tggtctgcaa ggcccgcttc gaagccttcg gcgccgccgg caatgccagc
      421 aagatcaagc ccgtgtccct cgacaagatc gccgcccgct acaagagcgg cgagctcaac
      481 cagatcgtgc gctgaacccg ggcacttcgc ccgccgtcgg cagtctcagg cagggccgga
      541 ctttccggcc ctgttttcgt ctggaggatc cccatggccg tcccctggtc caccctgttc
      601 ggcgccattc cctggtccga cgtcatcgcc cgcgcgcccg acatcgccca gggcgcccgc
      661 aaactctggc agaaggccgg ccggcgcggc gaccctccgc cggacgcaag ccccgtcgcg
      721 ccgagcctcg acgtccgcgt cgacgcactc gccgcccgtc tcggcgacat cgccgcacgc
      781 cagcaggaca gtgccgagct gctcgccgcc ctggccaccc agcaggccga actgatccgc
      841 gccaccgacg ccctgcaccg ccgggcgcgc ctgctgggca tcgcctgcgc catcctcggc
      901 cttgcactgg ccggcctcgc caccgcgatc ctggtgcgcg ccgcctgatt ttttgttgca
      961 ccgcaacaac aggcgtgacg cctgccacgc cttgtggttt tcccgacctt gccagctcaa
     1021 cttccgtaac ggcaagatgg gtgccccgat cgcgctccgc ggaggaccgc ccgatgacgc
     1081 ccacgaccct gtttgccgaa cacgacctgc gccagaccct caacgacgag ttccacgccc
     1141 gtccgccggt gccgctggaa agcccccagc tcatcagcta cctggccttc caccagcagg
     1201 acgtggccgc cggcgaagcc cagcgccacc tggccgaact gggtgagcgc ctgggtcgcc
     1261 cgctcgatgc cggcggcccg cactgcctgc tggacttcgg cgccttccag ctcaagtggg
     1321 agctccacaa cgaattcacc agctacacct tcatccgccc cgtggcgcgg ggcgaaggac
     1381 tggacggcag cacctcggcc ctggccgtcg tgccccagga ctggctgcgc gcgatccccg
     1441 gccgcctgat ggtggccacc cacgtggagc tgcgctccgc cgccgaggtg agcccggagg
     1501 aggtgatggc ccagctttcg cccaccggcc ggcagatggt gggcgcgatg gtggccgacg
     1561 gtgccgcctg ggtgttcacc gacttcctct tcgaggacgg cttctcgcgc ttcctcgtca
     1621 tcgacgtgtc cctcacccgc cgccaggccg gccgcaccgt acagcgcctg gtggagatcg
     1681 agacctaccg cctgatggcc ctgctcgcct tcccggtcgc caaggacgtg ggacgcctcc
     1741 tgggccgggc cgagagcgat ctggcgcgcc tcatggaccg caccggttcg gcccgctccc
     1801 ccgaggacga gcgcggcgtg ctggccgacc tgtcggccct ggccgccgag gtggaacact
     1861 ccgtagcgcg caccaccttt cgttttggcg cggcgcgggc ctatcacagc ctggtcatgc
     1921 agcgcatcga agagctgcgc gagcgccgca tcaccggcct gcccaccttc cacgagttca
     1981 tggaacgccg cctgctgccc gccatgaaca cctgccagac catggcgcgc cgccaggaag
     2041 acctgtccgg tcgcgtcgcc cgcaacagcc agctcctgcg cacccgcgtc gaggtggaac
     2101 tggaacgcca gaatcaggaa ctcctcgccc agatgaaccg ccgcgccaag ctccagctcc
     2161 acctgcagga gactgtcgaa gggctgtcgg tggtggcgat cacctactac gcgtcgcagc
     2221 tcgtgcagta catggccaag ggcgtcaagc acttcatcga gcccctcacg ccggaaggcc
     2281 tcaccgccgc cgccatcccg gtgatcgccg gcatcgtcgc cctgggcctg caccgcatgc
     2341 gcaaggcgct tgccgccgcc gacggcggtc actgaacacc tccgccgcac cggggccacc
     2401 gggtggccgt gccggcgggc cgggctataa tcgcgttttg tccgccggga tcctcaccgt
     2461 gaccacgcct ctcttcgaat cgtccctgcg cagcctgccc ctgctcggcc gcggcaaggt
     2521 gcgcgacatc tacgccgtcg acgccgaccg gctgctcatc gtcacgtccg accgcctgtc
     2581 cgccttcgac gtgatcctgc ccgacccgat tccgcgcaag ggcgaagtgc tcaccgccat
     2641 ggccaacttc tggttcgccc ggctcggcca catcatcccc aaccagctca ccggggtcga
     2701 tccggaaagc gtggtggccg ccgacgagcg cgaccaggtg cgcggccgcg ccctggtggt
     2761 caagcgcctg cgtcccctgc ccatcgaggc ggtggtgcgc ggctacgtga tcggctcggg
     2821 ctggaaggac taccaggaca ccggcgccat ctgcggcatc gcgctgcccg ccggcctcaa
     2881 gcaggcccag aagctgcccg ccccgatctt caccccggcc agcaaggccg acgtgggcga
     2941 ccacgacgag aacatctcct tcgaacaggc ccaggcccgc actgctgccc agtttgccga
     3001 cagtggcctg gacggcgccc gcctggtgag tgaagcccgt gacgccgccc tcgccctcta
     3061 ctcggaagcg tcggcctacg ccgccggccg cggcatcctc atcgccgaca ccaagttcga
     3121 attcggcgtg gacgccgccg gcaccctgca cctcatcgac gaggccctga cgccggactc
     3181 ctcccgtttc tggcctgccg acagctacgc cgagggcatc agtcccccca gcttcgacaa
     3241 gcagtacgtg cgtgattacc tggaaaccct cgactggggc aagaaggcgc cgggcccgac
     3301 gctgcctgcg gacgtcatcg cccgcacctc ggccaagtat gtggaagcct acgagcgcct
     3361 caccggtctc agcctgtaag ccccacgcag ccgctcccgg ccggcgccgg ggcggcgcgc
     3421 ctcacaggtg gatgctgaag caggcgatca gcgcctcccg ctcttccgca gactccttca
     3481 acaccgcgcc gatctccaca tcgtccagcc ccagggtcgc caccttggcc agaccgttgc
     3541 ggaagggctc cggctccaga gagaacggat tccccgtctc ggccagcagg ttggcgatga
     3601 agatcaggtc cgccacattc tccggcggcc agcgtccgcc gtagagttcc ggatcgtcca
     3661 ccgcatccac cagatcctgg ggcatgtgca gggaggccag cacggcccgc ccgatgggcg
     3721 gatgccacac ccgcaccaga tccgacaggg gactgccctc ccgcagcagc tccgggtatt
     3781 cccacacccg cgcgagcagg aagaactggc cgatgtcgtg gaccatgccg gcgaagaagg
     3841 cctcgtccgg gttgatccgg ctgcaatgcc gcgccagcac gcaggcggtg gccgccacat
     3901 ccacgcaatg ggcccacagc acctgcatct ggggctggaa ggggcccagc atgtccttgg
     3961 tggcgagctg ttcggccgcc acggtaagcg ccagggcccg cagtttggcg attccgatgc
     4021 gcgccagcgc ccgtggcacg tccgccacca gccggccccc cgggttgtac caggcggagt
     4081 tggccagcgc cacggtgcgc gccgccagca ggggctcggc cgacaccatg cgggcaatgc
     4141 gcgcaatgtc cgcgtccgga tcgtcgagca cctccatgac ccgcagggtc acttccatcg
     4201 cggtgggaaa cttcagttcc ccgcgttcca gctccatcgt gatggtgtgc agaaaagcct
     4261 gttccaccag gctcagttcc gattcatcca tgggaccgcc acaaaccttc ccgccgaccc
     4321 gcgacacccc gcacggaatt cggcacaata aaaagatcca ccccaccgac acgcaacaat
     4381 gaccaccgcc actgctccgt ctcccctgct ccagcccacc ggcctgccgc gcttcgacgc
     4441 catcacgccc gaccaggtcg aacccggcct gcgcagcctc ctcgacgcct acggcgccac
     4501 cctcgcccgc ctgcaggacg atccgacgac gcccacctgg gcgaatttcg tcgaacccat
     4561 cgagctcgaa ggcgagcgcc tcggccgcgc ctggggcgtg gtggtgcaca tgcacagcgt
     4621 gatggacacc ccggaatggc gcgaggccta caacgcaatg ttgccggagg tcacccgctt
     4681 ttacgccgaa ttggggcaaa accttaagct tttcgaaaaa tacaaggcta tcgccgaagg
     4741 tcccgaattc gccggcctca cgcccgcccg ccagcgcatc ctcgacaacg agctgcgcgg
     4801 cttccgcctt tccggcgccg aactcccgga cgcccggaag ccccgcttca aggaaatcca
     4861 ggaagaactg gccggcctct ccgccaggtt ctccgaaaac ctcctcgacg ccaccaacgc
     4921 cttcagccac ctcgtcaccg acccggcccg cctcgccggc ctgccggacg acgtagtgac
     4981 cgcggcccgc gaagcggccg cgaaggccgg ccaggaaggc tggaaattca ccctcaagat
     5041 gccctcctac ctgccggtca tgcagtacgc cgacgaccgc gccctgcgcg aaacgatgta
     5101 ccgcgccaac ggcacccgcg ccgccgagat cgacaacggc ttcagcaagc ccgagtggaa
     5161 caacggcccg ctgatcgagc gcatcctcgc cctgcgcgcc gaagaagccg cgatgctcgg
     5221 ctaccgcacc tacgccgacg tctccctggt gcccaagatg gccgactccc ccgagcaggt
     5281 gctcggcttc ctgcgcgaac tggccgccaa ggccaggccc ttcgccgagc gggacctggc
     5341 cgaactcgcc acctttgccc gcgacgaact ggggctcgac cccctcgaac cgtgggacac
     5401 gggctacgcc tccgaaaaac tgcgggtcgc ccgctacgac ttctccgagc aggaggtgaa
     5461 gcagtacctg cccgaaccca aggtcctgca gggactcttc gacgtcgtgg agaagctcta
     5521 cggcgtgcgc atcctgcccg acagcggccc ggtgtggcac ccggatgtgc gcttcttccg
     5581 catcgagcgc cagggcgcgc tggtcggcca gttctacctg gacctctacg cccgcgagac
     5641 caagaagggc ggcgcctgga tggattccgc catcagccgg cggcgcctcg gcgacggcat
     5701 cgagacgccg gtcgcctacc tggtgtgcaa cttccccggc ccggtcggcg gcaaggctgc
     5761 caccttcagc cacgacgacg tgatcaccct cttccacgag gccggccacg gtctgcacca
     5821 tctcctcacg cgggtggacg acctggccgt ctccggcatc cacggcgtcg aatgggatgc
     5881 cgtcgagctg cccagccagt tcatggagaa cttctgctgg gaatgggacg tggtccgcga
     5941 gatgaccgcc cacgcggaca ccggcgaggc cctgccccgc gccctcttcg acaagatgct
     6001 cgccgccaag aacttccaga gcggcatgca gaccgtgcgc cagctggaat tcgccctctt
     6061 cgacctgcgc ctgcacctgg acttcaaccc ggccaacccg cgcgtcatgg acctcctcga
     6121 cgaagtccgc caggaagtgg ccgtgctcgt cccgcccgcc tggcaccgct tccccaacag
     6181 cttcagccac atcttcgccg gcggctacgc agcgggttac tacagctaca agtgggccga
     6241 ggtgctctcc gccgacgcct tcgccgcctt cgaggaagcc ggccggcccc agggcagcgt
     6301 gctggacgcc gtcaccggcg cgcgcttctg ggaagagatc ctcgcggtcg gcggcagccg
     6361 tccggcgctg gagtccttca aggccttccg cgggcgcgaa ccccaggtgg acgccctcct
     6421 gcgccacagc ggcatggtgc ccgcatgaaa cgccgtagcg ccctcctcct gctgatcgcc
     6481 gccgcgccgg ccctcgccca gaccgcctat cgctgggtgg acgacaaggg ccgcgtccag
     6541 tacagcgacc agccgccccc cgcggcggtg ccccagtacg accagcgcaa catcagcgcc
     6601 aaccagagcg gcagcctcca gcaatccttc gccacccgcc aggcggccgg caaatacccg
     6661 gtcacgctgt acgtctccaa ggattgcggc aaggcctgcg aggacgggcg ggcattcctc
     6721 aagaagcgca cgatcccctt cagcgagcag cgcatcgaaa gcgccgagga cgtcgcgacc
     6781 tacaaggccc gcatcggtgc agagcccttc gtaccgaccc tcctggtggg gcgcgagaag
     6841 gagaccggct tcgctgcgcc cgcctgggac aatctgctcg acaaggccgg ctaccccgcc
     6901 gcacgccgct gacgcaccgt tctgcgacgc ccgcggaccc gcgccgcggg cggacgacag
     6961 acattgcctg cctcagccgg cagatccggt gcctttccct ccggaataag cctttcgtgc
     7021 tatgactttc gttcaattgt cataacacgt ataactctat acagtctcca cctcccgatc
     7081 gtcatggaga ctgccagtga aaaccagaaa ccgcctgatt gccgaagccc tggggggcgc
     7141 cctgttcctg tccgcctgcg gcggcggagg agggggcagc agcaccccgg ccagccccca
     7201 gacgccggcc agcgtcgacg tcaccctcac cccgtccctt ggcaagttct ccaccggctg
     7261 cagcgtcgag ctgcgcaccg gcagcgggac gctcctcggt tcggcgccca tcaccgacgc
     7321 cggcaacgcc ttcatccgcg tcaccggcta cagcggcccg gtcatcgccc aggtcaaggg
     7381 cgccagcggc tgcacctact tcgacgaagc caccgccagc caggtcccct tcggcgccga
     7441 ccgcagcctg agcgccgtct ccgacaccct gcgggtggac cagggcatca acgtcctcac
     7501 ccacctggcc gccagccgcg tgctcgacgg cgacaagctc gccaccggca agaccgccga
     7561 caccgtcaag ctggaaaacc tcaccatcca gcgcatgttc caggtggccg acatgttcgc
     7621 ggcgcccacg ctgctgggca acgcctcaga ccggctcgac aacaccgcgg cgggccagct
     7681 tgccgtgcag ctcgccgccc tctccgaact ggcccacacc cgcgccacgg acgtcgccac
     7741 cctcgccgcc acgctggcca ccgacctcgc cgacggcacc ctcgacacct ttggcgccag
     7801 cgaataccag gtcgccgcgc agaccaccgt cgccaagctg gcctccccgt cggcccaggc
     7861 ctccctcctc atcctgagcc agacccccag cctcatcgtg agcacccgcg atgtgcagtc
     7921 ggacgtgcag aaggtcatcg ccgccggccc ggccctcgcc caggcgaagc agctcttcgc
     7981 cgacctgcgc accagcgtgc tcgccctcag caacagcgcc ggcaccggca ccctcgacgc
     8041 cgaaaacgca ctgctcaagg ccgacttcca gcacggcctc ggcgtggaca tgctgatcga
     8101 cggcgtcaaa ttcatgctcg aatccgccag ccagttcttc gattccggca cctccaggcc
     8161 cggcagcacc catagcggct gggtcggcac ctgctaccag gactccacga cccaagccac
     8221 ctgccatgtt tcgggcgtag acgacggcag cgtctacacc agctaccaag tccgcctgac
     8281 caagaccggc gccggtacgg cctcgtgggc cttcacacag gtgtaccgct accagaacgg
     8341 gcagagcacc cagaccgccg cgccctccgg catgagcggc agcttcacgt actccgccaa
     8401 tgccgccacg gccaaggggc agttcatccc catgaccggc gacgccgagc gcagcgccat
     8461 cgacatcagc ctcggcttca ctaccacagg gaccaccggc gccatccgat tcagcgccac
     8521 cggctcggcc aattcggtca agaccgacgg caccagcacc ctccaggtgg atctcaccaa
     8581 gctcgacctg gacccggtca gtcaggtgaa ccagatggtg ttgagcctca ccggcccccg
     8641 tcaccgcttc gacggcgcac tcaccctgag cggccaggtg gcctcggccg atggcagcat
     8701 cgcccagccc aagtcgctca agttcgtcgg caccttcacc gacaccgcca gccactacac
     8761 ggtcctcgac ggcaccgtca ccgcgtccca ggactggacc ggctacgacc cccgcctggc
     8821 caactccgac ggcaacttcg cccgctatgc cttcagcttt gacggcgtcg ccaacaaggg
     8881 accgggtgcg gcggggatca ccctgtcctt cagcggcgac aaccaggcag ggccccacga
     8941 ccagaagatc gcgctcaagt tcaccagcgc cagcggcatc gtcgtgaccg gcaccggcgt
     9001 ccagacgccg gcacgccccg acgacttgtg gaactggacc ttcgccaacg agaacggcat
     9061 caaggcgact tacgcccaga gcagccggtc cggcaaggtc acggccggcg atggcagcga
     9121 cctgggcacc ctgagcagcc agcgcgtcac cttcgtggac ggcagtttcg aatcgctgat
     9181 gtaagcccat cgtcctcccg gccggctggc gccttcgccc gccggccgct ttccgtcctt
     9241 ctctcatgac gcccgctccg tgcgcacttc cccagtcccc gtcctgatca tccttgccgc
     9301 gctgcccggc ctcgcccggg ccgacgcggc cgaccttctg gccctggtcc agccgacgcg
     9361 caacgatgcc gccaccgccg tgcgggtgga cgcactgggc ttcgccgccg acgacttcac
     9421 gcccgcccac agcatcgcca ggacctggaa cgcgccgccc accccacggg acggccccaa
     9481 ccgggccctc ggattcgccc gcctcgagac ctccgcctcg cacggcacct ggagcgtcgc
     9541 cgtcttccgt cgctacgatg ccctgggcag cggtacgccc gatacggtgc gcttctacca
     9601 cgccctcgcc cacccggacg atctgctcgc ccgcgaccag cgcctggcca tcgactaccg
     9661 cttcaccggc ttttcggccg acggcttgcg cctgacccgg cacttctccc ccgcgcccga
     9721 tcaagcccgt tacgcggtgt ccctcaacct gctcaaggcc ggcgccctgc gcatggagcg
     9781 ggccactggc atcgccacca ccgccggtgg cgtggccacc gtcaatggcc gtcggagtct
     9841 gctgtacacc ggcctcgatc cgacaccgac atcccaggcc catctcaacg atttcgcccc
     9901 cgtcaccacc ggtacgccga gcgcaggctg gggggtcagc gtggacctgg atgcccggtg
     9961 gcagccccgt ccggacctgc acctgagcgt cgccaccaac gatctcctcg gccgcctgca
    10021 ctggcaggac gtgcccgaaa tggtccagga cttcaacggc accgcctggc cgcttcagtt
    10081 caaccagccc caggccaccg cccgcatcac gggcgccaac cattaccggg acgtcacact
    10141 gcacctcaag cccaaggtgg ctgccgccgc agaatggtcg gtcagcccgc gctggcaggc
    10201 agacgccagc ctcgccacgg tccagggcgt ggtgctgccc gaggccggcc tgagctggca
    10261 ggccaccgca gggccccgcg tgcggctgtc ctacgagacc cgcttcggca gcgccggcgt
    10321 gggcgtccgc atgggcccct tctacgccga agtccgcagc gaccggttca atctcaatga
    10381 cgcccacagc cttggcgcac gggccggcct gacagtggca ttctgaggcg ccacgcccca
    10441 gacacccgcg ccgacctgtc gtgacgcgat cctgaaaccc ccgcatcttc aaggcgtttt
    10501 accctgaatc ccggcctgcc cgcgtcatcg acaccacccg gaatggccgc acctgcgatc
    10561 gccccgcctg gcgcccctgt ccgccctcgc ccccagtcag caggctggca tcgaacagac
    10621 ctcaggcgcc tccacaccgg acgcaccctc gcccggcgag cccttcacct gccgatgtct
    10681 gtgcccctgg gcgactcgag cgcgaccggt cacgccatgc ccgacgacgc cccctcgcca
    10741 tccaccgcgt tgccgtatcc tcaagccgac gtaccgctgg cacaatcacg tccattcccg
    10801 gtccgccgaa acccggcggc gccatttttc cgcggctcgt ccgcacagcc cgacgccccg
    10861 cccccatgaa aatcgccacc tggaacgtca attccctcaa ggtccgcctg ccccacgtgc
    10921 tcgactggct ggccgcggcg cagccggacg ctctgtgcct gcaggaactc aaatgcgaga
    10981 acaaggcctt tcccctctcc gagatcgagg ccgcgggcta ccacgccgtc ttcaacggcc
    11041 agaagaccta caacggtgtc gccatcctca gccgcacgcc ggccgcagag gtcagcctgg
    11101 acatccccgg cttcgcagac gagcagaagc gcatcatcgc cgccaccgtc gagggcgtgc
    11161 gcgtcgtgtg cggctacttt cccaacggtc aggccgtcgg ctccgacaag ttcgcctaca
    11221 agctggcctg gctcgcggcc ctcaccgact ggctgcgcgg cgagctgaag acgtcaccgc
    11281 gcctcgtgct ggccggcgac tacaacatcg ccccggaaga ccgggacgcc cacccggact
    11341 ggaaggacga gatccacgtc tccgccccgg agcgggaggc cttccgcacc ctgctcgacc
    11401 tgggcctgag cgacgccttc cgcctgttcg agcaggcgga caagagcttc tcctggtggg
    11461 attaccgcat gggcgccttc cgtcgcaact tcggcctgcg catcgaccac ctgctggtct
    11521 cgcccgcgct gaaggacgtg tgccgtgcgt gctacgtgga caaggccccg cgcaagctgg
    11581 agcgcccctc ggaccacgcc ccggtcgtcg tcgaattcgc atgagcaccc tcacgccggg
    11641 cgacgaactg gccgccctct accccgtcct cgccggtctc ccggatgccg cccgccgccg
    11701 gttgggcaac cacgcgcgcc gcatcacggt gcccgcaggt accgccctct tcgacgagcg
    11761 ccaggcctgt cagggcttcc ccttcgtcct cgccggcagc atccgggtca tcaaggccgc
    11821 gcccagcggc cgcgagcttc ccctctaccg ggtggcgcca ggggagacct gcatcatctc
    11881 ctcggcctgc ctgctcggcc atgccgccta caatgcccgc ggcgtcaccg agaccgacac
    11941 cacattactg ctcctgcccg acaccgactt ccagtccctg ctcgccgaac cagccttccg
    12001 cgacttcgtc ttccagctct tctccgagcg catcgccgac ctgatgcagc tcatcgagga
    12061 agtggccttc cgcaagctcg accagcgcct cgccgccctg ctcctgggca agggccagcg
    12121 cctgcacacc acccatcaac agctcgccga tgaactgggc agcgtgcgcg agatcgtcag
    12181 ccgcctgctc aagggcttcg ccgaacaggg cctggtggcg ctgtcgcggg agcagatcga
    12241 gattcgcgac gctgccggcc tgcgccgcgt cgccgccggc tgacgcccag cccgaatgca
    12301 tcggacggcc cggcgtcctg caggcgccgc agcggacgat gtcccgcccc cggctgccct
    12361 gcgcgccgtg tccatgcccg tccgttcaaa ccaatcaccg gcactgccac cggcacccgc
    12421 cgcctctgta actgaggtta cagaccgcgc ttccgcctcc tgctgaaata cgctcacctg
    12481 acaacccttc cccggaggac atcatgaaga gcaatgtggg cggtatcgac aaaatcctgc
    12541 gtattggcgg cggcgtggcc ctggtggcct gggcggccct cggcgggccg gcatgggcct
    12601 ggatcggcgt ggtgcccctg gccaccggcc tggtgggctt ctgcccgctg tacccgctga
    12661 tcggtctcag cacctgcccg atgaagaagg actgacccct gcagggcgcc ccttgggcgc
    12721 ctttcggtcc atcgcgcggg ggcgctccgg aagtcacccc gcgcgtgggt acagccggcg
    12781 ccgagggcct catcgagccc ccagtccgac ggcgcgaaac ttgcatggac agtgcatcgt
    12841 ccttccgttt cgtcgcagtc atgacaaaga tcggcctctt cttcggcacc gaaaccggca
    12901 ccacccgcct ggtcgccaaa cgtctgcaca agcagcttgg cgacgacatc gccgacaaac
    12961 ccgtcaatgt gaaccgcatc gagccggccg agttgctgcg ctacgacgcg ctgattctgg
    13021 gcacgcccag ctacggcgaa ggccagattc ccggcgccgg cgccggctgc ttcgagccca
    13081 actgggaaga attcctcgcc aaaatgcctg ccgacgccga ccttcgcggc aagcgggtgg
    13141 ccctctttgg cctgggcgcc caggagcgct actcggaacg cttctgcagc tccctgttcg
    13201 ccctctacca ggtcttcaag ggctacggcg ccgagatcgt cggcgcctgg agcaccgggg
    13261 gctacacctt ccagcactcc gccgccgtgg tggacgggca gttcgtgggc ctggtgatcg
    13321 accagcgcac ccagggcatg ttcaccgacg accgcatcgc ccagtgggtg gcgcaggtca
    13381 aaccactgct gctggaaaag ctcaccgcag ccgcctgacc ctgctccggg ttgccgccgg
    13441 gccgctccgg cggcggcacg cgttcagatc tgcgcgtcac gcgccgccac gtgattggcg
    13501 atgcgcacga aggctgccac gtccagcgcc tcgccgcgca ggcccggatc gaggccgagg
    13561 gccacgaagt ccggatcgga caggatgtcc cgcagcgtgt tgcgcagggt cttgcggcgc
    13621 tggccgaaag ccgcggtgac gatgcgtccg agcagggcct cgtcgatggc ggtacattcc
    13681 gccagcggcc gcggcaccat gcgcacgatg ctggacatca ccttgggcgc gggccggaag
    13741 gcgccgggtg gcacgtcgaa caggcgggcc atcgcaaaac ggtactggag catcaccgac
    13801 agtcggccat agtcctcggt gcctggcgca gccaccatgc gcatcacgac ttccttctgc
    13861 agcatgaagg tcatgtcttt cacccgctcg gcgctgtcgg ccagatgaaa gagcaggggc
    13921 gtggagatgt tgtagggcag attgcccacc acccgcaggg gacggccgtc cgccgccagg
    13981 gcggcgaagt cgaacttcag cgcatcgccc tcgtggatgg tgagggtgtc gggcgcgtgg
    14041 gccgccttga ggcgcgcgat caggtcccgg tcgatctcca ccacatgcag atggtcgagg
    14101 cgcgccatca ggggctcggt catggccccc agacccggcc cgatctccac catgcggtcg
    14161 tccggtgcgg gccggatcgc ctcgatgatc ttgcggatga tgttggggtc ggacagaaag
    14221 ttctggccga agcgcttgcg cgccagatgg ggtgcgttca tcccgccttc ctttcccagg
    14281 ccgccagggc ctcgcggatg cgcggcacgg cggcaaagcc ggcccgctcc ccttccagga
    14341 tggcctcgtg acgctgctga aagttggtgg ccggcaggcc ttgcagattg ggacggatca
    14401 ccacgtcggc cgcggccagc tcgaccttgc cgagggcgcc gcccatgatg gaaatggtgt
    14461 ccagcagcac gtccacgctg ccctggttct tcttgccgct gggccggccg gagatgtcca
    14521 cggcgatcac caggtcggca cccaggctgc gcgcctcacg caccgggatg ggtgccacca
    14581 gcccgccatc cacgtattcc cggccattga ccgcgacggg cgggaagacg ccgggaatcg
    14641 ccgccgaggc gcgcacggcg atgcccgtgt tgccacgctg gaagacgatc acctcgccgc
    14701 tctgcagatc tgtggccact gccgcaaagc ggcggcgcag cccttcgatg ggcttgttgc
    14761 cggcctgggt gttgatgaag cgttccagcg cctcgccctt gatgaagccg cggtcgaaga
    14821 agctccagtc ggtcaccgcc gattcgtcga gctggatggc gagcttctgc aactcgaagg
    14881 gcccgtgacc cgccgcgtag agactgccga ccacggcacc ggcgctggtg cccaccacca
    14941 ggtccggctg gatgccgctc gtttcgaggg ccttgatcac ccccacgtgg gcaaagccgc
    15001 gggccgcacc gccgcccagg gccaggccga tgcgcgcacg gggacggggc gtcgcgggca
    15061 aaccgggcgc cggcaccttc gcggtggcgt ccggccgggt ccccagcccc ccgcaggctg
    15121 acagacccga ggccgccagc agcgccgcac ccgcctgcag cagcgcgcgc cgcctcatgg
    15181 ccggcctccg cggctcaggc tgatggccag ttcgaccgcg gcgaacaggc tgcccggatc
    15241 ggcgcgcccc gtgccggcga gatccagggc cgtgccatgg tccaccgaag tccgcacgat
    15301 gggcaggccg agggtgacat tcactccgcc gccgaagctg gcatgcttga gcaccggcag
    15361 gccctggtcg tggtacatgg cgagcacggc gtcgccgcgg gcgagggtgt ggggcacgaa
    15421 gagggtgtcg gccggcaggg gaccgacgag gtccatgccc tcggcgcgca gggcgtcgag
    15481 gaccggctcg atgacctcga tctcctcacg gcccatgtgg ccaccctccc cggcgtgggg
    15541 gttgaggccc gccaccagga tgcgcggcgc ggccagtccg aagcgctctc gcaggtcggc
    15601 gtggaggatg cgcagcacct cggcaaggag gggctgcgtg atcgcggcgg gcacgtcctt
    15661 gaggggcagg tgggtggtga ccagcgccac ccgcaggccg ccacccacca gcatcatgac
    15721 gaccaggggc gtgccggtgg cttcggccag gtattcggtg tgccccgaga aaggcacgcc
    15781 tgcctcgcag atgatgccct tgtgcacggg ggcggtgacc attccggcga actcgccgct
    15841 gcggcagccc gcgatggccc ggtcgaggat atccagcacg tagcggccgt tggcggcgtc
    15901 cagacggccc gcctctgcgg ggactgccag gggcacgtgg aggacttcca gggtgccgtc
    15961 acggggggct gcggacgggt cataggggac gacggcaaga tccggcgcca gcatctgcgc
    16021 ccgcgcggcg agcagctcgt gatcaccgag gacgaccgga cgggccgccc agggccggga
    16081 cggcagggcg acacacaggt ccggcccgac cccggcggga tcgccgctgg tgatggcaag
    16141 gacggggagg gaatcagaca tggggcttca agaaaattgc ccgccggaaa ggcgggcgga
    16201 aggatcacga cggaaatgac tcaagcgagc tgtgagactg tgatccggcc tccgcgttcc
    16261 cgccggatca cttgtcctcg aggcggatat ccacgtaggc ccggtcgcgc agctggcgca
    16321 gccagtcctg gtaggcttcg tcgagcttgc gctcacgcag ggcattgcgg gcggcgttgc
    16381 gcttgcgctc gtcggagaca tcctgcacac gccgctcctc cacctggatc aggtgccagc
    16441 caaagggcga ctggaccggt tgggagatct cgccgggctt gagggcgtcc atggcctgtt
    16501 cgaattcggg caccgtgtcg ccgggataga tccagcccag gtcgcccccc ttggaggcgg
    16561 acaggtcggc ggaatgggcg cgggccagtt cggcgaaact ggcgcccccc tgaacgatgc
    16621 gctcgcgcag atccagcagg cggcggcggg catcgccatc cgacaccact tcgctggtct
    16681 tgatgaggat gtggcgggca tgggtctgct gcacctgggt ggcgccgagg gcatcgccgc
    16741 cgccgcgacg gtcgatcagg cggacgatgt ggaagccggc agcactgcgc aggatgggcg
    16801 aggtttcgcc gggcttcatc cgggtaaccg catcggcaaa caggccgggc aggcgctcgg
    16861 cgctgcgcca gcccaggttg ccgccggaca gggcgtcggg cgcatcggaa tagctggcgg
    16921 ccagcttgtc gaagggctcg ccgcgggcca cccgggccgc cacatcctcg gcacgggcac
    16981 gcaggcgggc gagttgctcg ggggtggcgc cttccggcgc gcgcagcagg atgtgggcga
    17041 ggttgaattc ttcctggctc tgggtgtttt cgggatgagc gaggaaattg ctcacctcgg
    17101 catcggagac gacgatgcgc gactccacct cccgctcgcg caggcggctg aggagcatct
    17161 cggtgcggat ctcctcgcgg aagcggtccc aggtgatgcc atccttctcc agcgcctgac
    17221 ggaattccgt catggacagc ttgttgttgt cggcgatgcg cccgagggcc cggtcgaggg
    17281 ccagatcgtc caccctcagg gaggtctcgt tggccagctg gatctgcagc ttttccatga
    17341 tcaggcgttc gagcacctga cgctccagca cgtcgtcggc cggcaggggc gtgccctggc
    17401 gggtgagctg gcggctgatc tgctcgacct tgaggcgcag gtcggagcgg gtgaccacgt
    17461 cactgttgac caccgccacg atgcggtcca cctcaaccgg tccggccgcc caggcggcat
    17521 gggtgaagac gagcgtggca agcagggcaa ggagacgcat catgagacgg cccgattgat
    17581 caatagtcat cgttggtgaa gaaggggctg gagccggggt cgctgatctt gccataaccc
    17641 ggcacgctgc ggcggagcat gttgatgggg ttggagccca ggctggcgag cccgctgaac
    17701 tcgatctgca cgaagaaagc gcgggtggtg tcggtggcgg aggtggtcag atgctgcacg
    17761 acggtgcgcg ccacccagca gccaccgttg tactcgaagc cggcgatgcc ttcggtgagg
    17821 cggtggtccc gcaggttgcg gttgtagcgc cccacggcgt accagttgcc ggccaggggc
    17881 cactgcccgg tgaggtccag gtcgcggaaa ccgtcggggg tggcggccac ggtggaatag
    17941 ttgcggcgat aacgccagct ggcgttgagg gcgcgggcaa agcccggctg gtagcgcacg
    18001 ccggcattga agcgctcggt ccagtggtcc cgcgggttgt actgccagcc cgtgtccagc
    18061 tgggtgcgcg gcgcgatctg cccgccaaag gcggcgagga agtcggcctg cccgccggtg
    18121 cgggcagcca cgccgggcag ggtgacgcgc tgctcttcca gataatagcg ctgcccgaag
    18181 gcggcccgga tgacctcggc gccggtgttg gggttgatca gccggctctg caccgcggtg
    18241 gtgatctggt tggcgttgga gatgcgatca ccgccggtgt agtagttctc cgcgaagatc
    18301 tgcgcgaagt tgaagtcgta caccccggag tcgaagttgg ggatgttgga ctggtcgcgg
    18361 tagggcacgt agaggtaata gatgcgcggc tcgagggtct gggtaaattc gcgccccgca
    18421 atctgtgtgt cccgctcgaa attcacgccg ctgtccaccg acaggatggg caggctgcgc
    18481 tggatggaat ccggcccggt actggtgcgc cgatccaggt tgtattcggt ggcatgcacg
    18541 ccgaccttgg gtgtgacata gaacgcggac gtgaccagcg gcagggacag ctgcgggtac
    18601 agcgtcagac gccggccctc gtcctgtgtc ggatggctga aggccacgaa ttcgctgttg
    18661 aacaggaagg cggtgccccc cggcagctcg ggccggttgg ccgtgagggt cagctggggc
    18721 aggcgcttgt agggcacgga gacggcggcc ccgttgggat cctgcagggt ctgccaggcc
    18781 tgcagcatgc cggtggcgtt ccaccagccg ccaccgtaat accccacgct gccctgacgc
    18841 agcaggttga cctgggaggt gagggacagc cgggagctga gatcgatgaa gtagcgcgga
    18901 tcggacacgc cactcacgtc cacggcagtc acccagccgg tgggcgtcgt ctcgttgtga
    18961 cggatctgcc cggcgaggcg gctgcggttc tcgacctgat cgttgggcat ccattcgacg
    19021 aaggacatgc ccttcgcggt gggcgtcatg tagcgcactt cgtcgcggat ctgcagtccg
    19081 cggcgcccca tcaggcgggg cgtgatggtg gaatcgtagt tcggggcgat gttccagtaa
    19141 tacggcaccg ccaggtcgaa gcccaccttg ttggacgtgc cgaaactggg cgccagcagg
    19201 ccggactggc gctggtcacc cagcggaaaa tcggcccagg gcaggtagac gaccggcaca
    19261 tccttgaagt acagcgtgcc attgttggcc tcgcccacct cccggtcgaa gtcgagctgg
    19321 agttcccggg cgcgcaggta ccagtccgga cggtccggct tgcaactgct ccaggtgcca
    19381 ttgaggaagc ggaactgatt ttcgccttcc agctcgaggg actccgcgga tccatgcccg
    19441 gagatgggct ggcgccgccc ctgctgggtc gtgtcgaact gctgaccggg cctgggcggg
    19501 cggggcgggc ggaagatctc gtagctgggg gtatcgaagg ttccgacctg ttcctgcacc
    19561 ttgaagcgcg cgtggggccc ggtcatcaca tcgtcaccgc gggtcatgcg gacgttgccc
    19621 gtcgcctcca cctcgtcggt gagctcgaag tactggagcc ggtcgccttc cacacgggta
    19681 ccgtcccgca gcagcacggc gcgcccttcg gcggtgacgt caaaatcctg gcgcccaaac
    19741 accttgtcgg cggtgatcac ggattcgcca ttcttgaccg gggccggcgc agggccggag
    19801 cgggtcgcca ccgcagcggc caccggttgc ggcggcgcgg agggtcccgc cgccgggacg
    19861 ggtgcggtca cgactgccgc ggccgcagac gcggcaccgg gcgcctccga ccgcgcggac
    19921 ggcgcggcga tcaccggcgg cgccgcgacg gcgggttcgg ggactggcgc cgccttgcgg
    19981 gaacggatcg cctccggctt gcccagcagg gcggccggca cctcgaaggg ggggaggctt
    20041 tcggctcctg cgcgccctcc cagggcgaac agggcgacgg acaggaaacg caactggaaa
    20101 cgtggcttca tccggaggct ggcacggtga cggcgagcgg catcgagccg gtcgcgcggt
    20161 ttgataaact tgccaatttt acacgaacga taccctgact cccgtggaac gacgccaaca
    20221 actccagtcc tggcttgccg ccgaattccc cggccgcgcc ttcgatctcg ccccggccag
    20281 tgccgacgcc agcttccggc gctatttccg cgtcaccttc gccgacgatc cgcagcccgt
    20341 gatcgtcatg gacgcgcccc ccagccacga agactgccgt ccctacctgc gcgtcgccga
    20401 gctcttcggc ggtgccggcg cccatgtgcc cactgtgcac aagcagaacc tcgaagaagg
    20461 cttcctgctg ctctccgacc tgggcgccac cacctacctg gacgccctca ccgccgacaa
    20521 cgccgcccac ctctacgccg acgccctcgg cgcgctgatc tgcatccagc acgccagccg
    20581 ccccggcgtg ctgcccgact acgaccgggc gctgctgctg cgcgagatgg aactcttccc
    20641 cgtctggtac gtgggccgcc acaagggcgt caccctcagc gatgagcagt cccaggccct
    20701 ctacgacgtc ttcgaccgca tcgccgacgt caacctggcc gaacccaagg tcttcgtgca
    20761 tcgggacttc cacagccgca acctgatggt gtccacgccc aaccccggcg tgctggattt
    20821 ccaggacgcc gtctatggcc ccctcagcta cgacctggtg tccctcttca aggacgccta
    20881 cattcactgg gaggaggatt tcgtcctcga catgctcgcc cgctactggg aaaccgcccg
    20941 cgacctgggc ctgcccgtgc gcgccgattt cgcagaattc caccgggact tcgaatggat
    21001 gggcgtgcag cgccacatca aggtcctcgg catcttcgcc cgcctctatc accgggacgg
    21061 caaggatggc tacctgaagg acatgcccct cgtcatgcac tacctgcgca aggcctgcga
    21121 acgctacggc gagctccggc ccctgctgcg cctgctggag cagatcgatc ccctcgtcac
    21181 cgagaccggc tattccttct gaaccatgaa agcgatgatc ctcgccgccg gccgcggcga
    21241 acgcatgcgc cccctcaccg acacctgccc caagccgc
//
LOCUS       DAOQMM010000061        21239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_869_length_21239_cov_52.094505, whole genome shotgun sequence.
ACCESSION   DAOQMM010000061 DAOQMM010000000
VERSION     DAOQMM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_869_length_21239_cov_52.094505"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLH85_10820"
     CDS             <1..390
                     /locus_tag="PLH85_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNB65103.1"
                     /translation="TYQCRKPEDLAYTLAHLPELVVKEVHGAGGYGMLVGPAASTAEI
                     ERFRGLLTAHPARYIAQPTLALSNCPTFVESGVAPRHLDLRPFVLSGKDVDMVPGGLT
                     RVALTEGSLVVNSSQGGGTKDTWVLEE"
     gene            395..1354
                     /locus_tag="PLH85_10825"
     CDS             395..1354
                     /locus_tag="PLH85_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNB65104.1"
                     /translation="MLSRTADHLFWMARYIERAENLARLLDVTWQNSLVPQAPAAASE
                     NWRAVIALNSLETAYSAKHGNETTADKVLHFMVSDPANPASIFCCLRAARENAHAVRG
                     TITSEMWETLNATWIELRGQSHEQRMANGPGEFFDWVKMRASLNRGTTSGTMLQDEAY
                     NFIRLGTLLERADNTARILDVKFHVLLPVNHDSEDAITDFYQWGALLRSVSGFEVYRK
                     VYRDVITPERVAELLVLHPDMPRSLHFCLRGVVKNLDLLANSHSGETQRRAGMLYARL
                     RYGCVEDFLAGGLHEFLSDFMDRIYEIGAGINRDFLAPVEGLQ"
     gene            1351..2088
                     /locus_tag="PLH85_10830"
     CDS             1351..2088
                     /locus_tag="PLH85_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HNB65105.1"
                     /translation="MTYCVAMRLDAGLVFLSDSRTNAGVDQINTFRKMQVFEKPGERM
                     LVLLSAGNLSISQSVVNLLRERLEQSRGPNLHTVKNMFEAARHVGDCLREIHARDAQA
                     LRDFGIDFNASLILGGQIAGEEPRLFQIYAVGNFIEATRETPYFQIGESKYGKPILDR
                     VITSETPIDQAAKCALISMDSTIKSNLTVGLPLDMLIYERDALKVGKQVTITAQNAYF
                     NDLRNRWGECLRNAFEELPAPDWDASF"
     gene            complement(2085..2684)
                     /locus_tag="PLH85_10835"
     CDS             complement(2085..2684)
                     /locus_tag="PLH85_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HNB65106.1"
                     /translation="MENPQSLVHFWFGTDPDDAVTAARQAGLWWGKDGAVDAEMARRY
                     GHLPDDALAGRLDGWAATPAGRLALILVTDQFPRNIFRGTAQAFAFDATARRLCREGL
                     AHGQDRALRPIERVFFYLPLEHSEDADDQALSVTLFEALAAEVAVPLQPIFDGYADYA
                     RRHRDIVARWGRFPHRNAVLGRASTAAEDAFLQEPGSSF"
     gene            complement(2751..5027)
                     /locus_tag="PLH85_10840"
     CDS             complement(2751..5027)
                     /locus_tag="PLH85_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65107.1"
                     /translation="MNTAGPARTRVWQNEARLVPVITWIATVTALLVAIAAPTGYFLL
                     AREAELRDAAMAARLHAAFVTQLIAASPDAWHQEVGGLLDTDLAPSDVPEQRRIVDAA
                     QQPIATTGAPLGRLVVERAAALRGPDGAVGEVIITRSLRPTLVRTGLVALLGVALGFT
                     IYGSLRVLPLRALRRAVAALQREESKAREEAEEQLRVVFQNAVEGIALFTPDRRITSC
                     NPAVARMFGRPLEHLPGMFITDLLRPFSGEEQGTAFTTGQGETTAFRASGEAFPVEMS
                     VNEARLADRPHFIAILRDITERKQAERRLSYLANYDSLTGLPNRSLFRERLERAMGRT
                     RRNGRQLALMFLDLDRFKNINDTLGHEAGDQLLRHVANALIANLRSTDTVSRATLDES
                     TESITVSRLGGDEFTVLVEDITSPAAAAQVATRILAAIAQPFEIHGERLYISTSIGVT
                     LYPHEAEDLDGLVKQADMAMYRAKELGRNTYFFYNDELNTEMAERHALEASLRHALER
                     REFHLHYQPKACLRSGRVTGVEALLRWTPSSGQRIGPDRFIPILEDIGLINQVGTWVL
                     RTACAQIAAWKTAGLPPLRLAVNVSARQFRQPNLPEQIAGLLAEHGLAPADLEVELTE
                     SMLMEDSEQSRDILSRLGTMGIQLAIDDFGTGHSSLAYLKRFNVHALKIDRSFVKDTP
                     EDPEDSAIATAIIGLSANLKIRVVAEGVETQAQADFLQQQGCDEMQGYLLSAPLPPRE
                     FAIWLRQRTAAGAVDPVT"
     gene            complement(5034..5486)
                     /locus_tag="PLH85_10845"
     CDS             complement(5034..5486)
                     /locus_tag="PLH85_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65108.1"
                     /translation="MSLPFSSSRVRQSLCRAATLLVLLGAPFSVRSADEMVVIINPEN
                     PNVIDRAYVVKVYTGAVKGWPDGSPIFLLDYGQDSELRKTFYTAVIGKSPAAMRAIWS
                     QNIFTGKAMPPRVTQEDSEMKHIVASNRNAIGYIRASQVDKTVKVIER"
     gene            complement(5486..6628)
                     /locus_tag="PLH85_10850"
     CDS             complement(5486..6628)
                     /locus_tag="PLH85_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65109.1"
                     /translation="MLHLRQLARATALGVLSLPLPALALEAGGLDFTGTGFMTLAAGR
                     VLSGTHDASVDQGFRCPCFISDYAQRGVYESGQWRAGPDSRLGLQGTVSAAQGRFALT
                     GQAVARGAANGAADLEWLYATAELSGNLTAQIGRKRLPLFSYSEAQDVGFALPWVHLP
                     PQLYGWEIVNYDGASLVWREQWGAWGSAVQVFGGNETRHDSGYWQIYNGKFSRTDVRW
                     SDILGTEVTLSRDWLTLRAVRIQSYTQNKVVSLGETAFSDRKRQVIQGLSMGVDSGNW
                     FARGEFLYINRTQDYGKDYANLIAVGYRHGPWQPLLSRSDYRQQLDTAGVPERHGTWS
                     AVLRYDLTEESALKVQADVWTDKTEPGFGSLHGDARLLTISYDRVF"
     gene            complement(6849..8006)
                     /locus_tag="PLH85_10855"
     CDS             complement(6849..8006)
                     /locus_tag="PLH85_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65110.1"
                     /translation="MITGKGAFCALMFSAGILSAPAATADEGPRLSGSPKVEFTDSTA
                     ERIGQTRRVAITNVILEFQTKVFAEHITGRLSKMYLKRGDSFSDNELAGFDPRQLEQA
                     ANQVYKKLVTDLTAAGFEVVPEAQVLAQPAYQKLRETMGWPQGYHFGNKEGHSLIVGP
                     TALKPYVPPLSEQGTFANTRQEKGELQAPALGWSDRARFAGNSSYAAGLEVQLAKALN
                     AHVVKVWYLIGFGNATASTDWDAISTAHFSTTATSRTGATAQMYLRDEETRLAVRLPD
                     GDAGYARESKRINAITGGYRPYDGDVVVRLDETIPGEADFLAGGGVQNATTERDAKAG
                     IGGLLGSMAKSLTGTIADNSYKTTVDPTRFAAAAADMAGQLQPFLIARIKR"
     gene            complement(8187..10325)
                     /locus_tag="PLH85_10860"
     CDS             complement(8187..10325)
                     /locus_tag="PLH85_10860"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNB65111.1"
                     /translation="MNKKLTWSLAAIAFATAAAALETATPPNRLAPTQDHAQTAYLSS
                     QLLARYHYKAVPLDDALSEKIFERYFKSLDPERLFFTQADIDAFADARTTLDDAILQG
                     NLGVPFAIFQRFEQRVLERLTQAREVLAGSFDFNVKESYGYSRDKAPWPKDDAEARDL
                     WRKRVKNDWLRLRLAGKDDKAIRETLEKRYDNSLARAGRNKADDVFQIFMNAYAMSIE
                     PHTNYLGRAASEDFDIGMRLSLMGIGAMLQERDEYITIRELVPGGPAARSGQLAPGDR
                     IVGVGQGESGALVDLLGTRVDEAVKLIRGEKDTKVRLDILPADAGPDGRHKQVVLVRD
                     KIKLEEQAAKKSILKVGEGAGARKVGVITLPAFYEDFEGRRRGDKDYRSASRDVARLL
                     DELRKDKVDGVLVDLRNNGGGSLREAVELTGLFIDKGPVVQQRRADGRIGVESDERAG
                     VAWDGPMGVLINRGSASASEIFAAALQDYGRAAILGEPSFGKGTVQTLVNLDEVVRND
                     KPRFGELKMTVAQFFRVNGGTTQLRGVTPDIRLPSVSDDESYGESSFDNALPWVKIDA
                     APFTPAGNVVPLVPDLQRRHQARVARDKDFQYLLEDVAEFRRLRDSKQISLNEAERRK
                     ERDTQEARLKAREKTGTKDKLANLRDDGLQAGERSLAADLAAEKARKERKDVLLQEAA
                     SIVGDEVDLQASATPRLALKERRPGQPVIR"
     gene            complement(10479..11300)
                     /gene="nifM"
                     /locus_tag="PLH85_10865"
     CDS             complement(10479..11300)
                     /gene="nifM"
                     /locus_tag="PLH85_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HNB65112.1"
                     /translation="MNPMLHGYLELKLAWEMFQKAPDALSEPERIRVGDIARKQDRIE
                     LRILASAEAAQVAIPAATLRQRVDEIRGRYPSEDDLIRDLERIGLSEGELDKAVERDL
                     RVEGVLEKVASAAEPVSAVDAEIYYRLHPEAFDRPEARRLRHILITFNGGNEKARAHK
                     TLDGLRGLGSAEAFAKAALRHSQCPTAMDGGLLGVVKRKQLYPELEPAAFALALGAVS
                     EVLESPIGLHILRCDEIFPSGLMPFEEVCPRIIERLADKRRQEAQRAWIKSLPAA"
     gene            complement(11297..11767)
                     /locus_tag="PLH85_10870"
     CDS             complement(11297..11767)
                     /locus_tag="PLH85_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNB65113.1"
                     /translation="MMDARWEYGEAVRLTRNVRNDGTYPGLDPGAFLVRRGSIGYVVD
                     VGTFLQDQVIYSVNFLDEGKIVGCREEELIGADEPWTPSRFEFRDKVRAVKGLSVGGE
                     MLITAGATGEVIKVIRDAPGGVAYHIHFDSLPGRILQIPESLLESATAEAPAEE"
     gene            complement(11764..12105)
                     /locus_tag="PLH85_10875"
     CDS             complement(11764..12105)
                     /locus_tag="PLH85_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HNB65114.1"
                     /translation="MDDTLTLDEAMEELVSAEDFLNYFGVPYDQSVVHVNRLHILQRF
                     HDYLAKQAKEMPEDEAARTDIYRQWLTRAYTDFVNSDALTEKVFAVFQNTSTPEGGMT
                     SFVSIDKVFQS"
     gene            complement(12116..12640)
                     /locus_tag="PLH85_10880"
     CDS             complement(12116..12640)
                     /locus_tag="PLH85_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65115.1"
                     /translation="MDLLDFSECKLYFEEDLPPEAEEMLAQASREYGQPDAEIALLRA
                     NLMVPDNLTVLVGLYRFYFYQHRLEEALRVAERAMYLSGRQLSLPDWESLDESSLAIA
                     AAISFGLLRFYLLALKAASVVLLRLGRIEESRARLHVLAAVDSRDQLGASKLLEVIAT
                     FENPDESDLSLVAA"
     gene            complement(12648..13418)
                     /gene="cysE"
                     /locus_tag="PLH85_10885"
     CDS             complement(12648..13418)
                     /gene="cysE"
                     /locus_tag="PLH85_10885"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNB65116.1"
                     /translation="MPDAMTIIAPTPAQPGLLDLLKEDLACVFQRDPAARSTLEVLTT
                     YPGVHAILLHRVSHRLWRAGWRYPARFLSFLSRTLTNVDIHPGATIGHRFFIDHGACV
                     VIGETAEVGDDVTLYHGVTLGGTSWNKGKRHPTLASGVVVGAGAKILGPIVIGERVRV
                     GANSVVVKDVPADRTVVGVPGRIVDTRHARSEDGVDLNHNVLPDPVAKSIACLIERIE
                     TLEKELAELRHEAPPPDHSGQCQTCSAGDLCCEHESMH"
     gene            complement(13411..14550)
                     /gene="nifV"
                     /locus_tag="PLH85_10890"
     CDS             complement(13411..14550)
                     /gene="nifV"
                     /locus_tag="PLH85_10890"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HNB65117.1"
                     /translation="MPQTSFVTINDTTLRDGEQSAGVAFSLDEKLEIARQLAAVGVPE
                     LEVGIPAMGAEERDSIRAVAALDLAPRLMVWSRMHPDDIAACADLGAHLVDISVPASD
                     QHLAYKLKQDRAWLLRELPRYIGAALSLGLEVGVGCEDASRADLDFLCALAETAERAG
                     ARRLRFADTLGILDPFGTYERISTLRRATDLEIEIHAHDDLGLATANTLAACLAGATH
                     ANTTVNGLGERAGNAPLEEVVLGLRKLHNIETGIDLKGFPALSAQVASASGRAVPWQK
                     SVVGPGAFCHEAGIHVDGLLKHPDNYQGFDPREVGRSHRIVLGKHSGSRAVQVVFAEL
                     GIPLDEREAQALLTRVRAFVADHKHPPEAADLRRLLASERSHFHA"
     gene            complement(14967..15941)
                     /locus_tag="PLH85_10895"
     CDS             complement(14967..15941)
                     /locus_tag="PLH85_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65118.1"
                     /translation="MKDPQAWPEYCKSLAPAIVETCTHTAVNTGPAGLVAALGSVVPE
                     WKFRHAFSRGGWYRLGGVYDGAGERVSDSLEAWVDAELDARGGELEALLDDYAGQAHY
                     ASRLVGQTHYLVAPSGEGAGDFIQLEIECLQEVRAHALFATTPDSVEDLVDPPRAAGS
                     AALPPIGLPTYTFRRLQHVGDFLRRMLVQKPEPAPIHRLLTDWDRSSAGIASAFSNHW
                     VILTQEHQDRYQQPVLHARPLATLAGHPPEFSAGESTRGIALGDALIRFNRAVGYPMA
                     WYFHMLTTKAVPYWVAQAVVEDALAGFAYLPEKDVAVVRDWLHRPYSV"
     gene            complement(15960..16721)
                     /locus_tag="PLH85_10900"
     CDS             complement(15960..16721)
                     /locus_tag="PLH85_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HNB65119.1"
                     /translation="MKREDKGAADAGIGRLGLCSGCSYFPDFLNAGRCVPGDACIRAH
                     SGRQIDRFLRANPELSGAYLADLFWERRAIAARYAPVEAIRGLRSDPDEVVRRVVATR
                     IPADELDSMLGDPDREVRLSVASRLPEPLLTRMVQDPDYLIRATVARRLPHGQLSRLA
                     KDAEREVRKVVASRLPPFALHMLADDPEPEVRAIVAERALPGLAAALLQDSEWWVRLA
                     AVGNAPLEFLPPLTSDPELEVREAALARLADSQAA"
     gene            complement(16711..16974)
                     /locus_tag="PLH85_10905"
     CDS             complement(16711..16974)
                     /locus_tag="PLH85_10905"
                     /inference="COORDINATES: protein motif:HMM:NF040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HNB65120.1"
                     /translation="MITPEQLTLVADAVRADAAIASLRARFPDLHFTECSEDDVSPRY
                     RAALETDGHALYYISGATGHCLEVTNDAALATGILVAAKADEA"
     gene            complement(16977..17702)
                     /locus_tag="PLH85_10910"
     CDS             complement(16977..17702)
                     /locus_tag="PLH85_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNB65121.1"
                     /translation="MSDSPIASREAALRIALAARCLAGLDVRAFVGALIDKLEAPLTE
                     AKLGTLTVEDLRALLAGDFAEQGCHVGVDADTLKEAIRLLWGQGVGAGDFPEVEAYSD
                     GDMPGSIRVAIAANRGENLDGHFGSCERFLIYQVGLDAIRLVGVRSTVEADAAEDRNA
                     ARSELINDCQIVYVQSIGGPAAAKVVRAGVHPVKKPTGGPAREILTELQGRLSSPPPW
                     LAQIMGVPAASLARFEEELEAED"
     gene            complement(17769..17960)
                     /locus_tag="PLH85_10915"
     CDS             complement(17769..17960)
                     /locus_tag="PLH85_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB65122.1"
                     /translation="MAMKIDPDMCTACGDCKPVCPTKSISSGKIFYKIDASTCTECDG
                     HNDSPLCVDVCPSGCISPA"
     gene            complement(17977..18201)
                     /gene="nifT"
                     /locus_tag="PLH85_10920"
     CDS             complement(17977..18201)
                     /gene="nifT"
                     /locus_tag="PLH85_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HNB65123.1"
                     /translation="MANIMIRQGEKGLTFYLPKRDLEASIESIEFDSPEKWGGELKLD
                     NGGSYYIDPIAKPARLPISLRAKRLGAAED"
     gene            complement(18259..19827)
                     /gene="nifK"
                     /locus_tag="PLH85_10925"
     CDS             complement(18259..19827)
                     /gene="nifK"
                     /locus_tag="PLH85_10925"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HNB65124.1"
                     /translation="MQTADKIKPCYPLFRDDDYKKNLAEKREQFEERHEPSKVWETFL
                     WTTTKEYQDLNFQREALTVDPAKACQPLGAVLCASGFHKTLPYVHGSQGCVAYFRTYF
                     NRHFKEPCSCVSDSMTEDAAVFGGQKNMFDGLENAKAIYKPDMIAVSTTCMAEVIGDD
                     LNAFINNAKKAGHVPQEYPVPFAHTPSFVGSHTTGWDNMEEGILRYFTLNNMEGKKAG
                     TKQTINLVPGFETYLGNYRVVKRMLTEMGVGYTMLSDPTDVFDTPADGEFRMYAGGTT
                     MDEVKDAPNAITTVLLQPWQLEKTKKFVETTWAHDVPKLNIPMGVEWTDEFLMKVSEL
                     TGQAIPASLELERGRCMDLISDSHAWLHGKKFALYGDPDFVMGMVKILLEVGAEPTHI
                     LSHNANKRWGKAVQKLLDASPFGKLGKVYVGNDLWHMRSLCFTDKPDFLIGNSYGKYI
                     QRDTRHKGEEFEVPLIRIGFPIFDRHHMHRATTLGYEGMMSVVTQLTNAVLEQLDKET
                     IGMGTTDYNFDLVR"
     gene            complement(19963..>21239)
                     /gene="nifD"
                     /locus_tag="PLH85_10930"
     CDS             complement(19963..>21239)
                     /gene="nifD"
                     /locus_tag="PLH85_10930"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HNB65125.1"
                     /translation="SKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYVGTTGVDTFG
                     TMNFTSDFQEKDIVFGGDKKLAKLIEEIETLFPLHKGISVQSECPIGLIGDDIESVSK
                     KAAVAIEKPVVPVRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGQAYDSTPYDVAIIG
                     DYNIGGDAWATRIILEEMGLRVVAQWSGDGTIAEMMNTPKVKLNLLHCYRSMNYISRH
                     MEEKYGIPYMEYNFFGPTKIVESCRKIAAFFDDSVKEKTEAMIARYQVMMDEIIAKYK
                     PRLQGKKVMLYVGGLRPRHVIGAYEDLGMEVIGTGYEFAHNDDYDRTIKEMGDATLLY
                     DDVTGFELEEFVKRLKPDMVGSGIKEKYIFQKMGIPLRQMHSWDYSGPYHGYDGFAIF
                     ARDMDIALSNPTFKNLTPPWKKAAEEDVKKAA"
BASE COUNT         3290 a         6447 c         7734 g         3768 t
ORIGIN      
        1 acctaccagt gccgcaagcc ggaggatctg gcctataccc tggcgcacct gcccgagctg
       61 gtggtgaagg aagtccatgg cgccgggggc tacggcatgc tggtcggccc ggccgccagc
      121 acggcggaga tcgaacgctt ccgcggcctg ttgacggcgc atccggcgcg ttacattgcg
      181 cagcccacac tggccctgtc caactgcccg accttcgtcg aaagcggtgt ggccccgcgc
      241 catctggacc tgcgcccctt cgtgctctcc ggcaaggatg tggacatggt gccgggcggc
      301 ctgacccgcg tggcgctgac cgaaggttcc ctcgtcgtga attcatccca gggtggcggt
      361 accaaggaca cctgggtgct ggaggaataa gaacatgctc tcaagaaccg ccgatcacct
      421 cttctggatg gcccgctaca ttgagcgggc agagaacctg gcccgcctgc tcgacgtgac
      481 ctggcagaat tccctggtgc cccaggcgcc ggccgccgcg tccgagaact ggcgcgcggt
      541 gatcgccctg aacagtctgg agacggccta ctccgccaaa catggcaacg agaccaccgc
      601 cgacaaggtg ctgcacttca tggtgagcga cccggccaat ccggcgtcca tcttctgctg
      661 cctgcgggcg gcccgtgaga atgcccacgc ggtgcgcggc accatcacgt ccgagatgtg
      721 ggagaccctc aacgccacct ggatcgaact gcgtggccag tcccacgagc agcgcatggc
      781 caacggtccg ggcgagttct tcgactgggt caagatgcgg gcctccctca accgcggcac
      841 cacgtccggc accatgctgc aggatgaggc ctacaacttc atccgcctcg gcaccctgct
      901 ggagcgggcc gacaacacgg cgcgcatcct tgacgtgaag ttccacgtac tgctgcccgt
      961 caaccacgac agcgaggacg ccatcaccga cttctaccag tggggtgccc tgctgcgttc
     1021 ggtgtccggc ttcgaggtct atcgcaaggt ctatcgcgac gtgatcacgc cggaacgggt
     1081 ggccgagctg ctggtcctgc acccggacat gccccgttcg ctgcacttct gcctgcgcgg
     1141 cgtggtcaag aacctggatc tcctcgccaa cagccattcc ggcgagaccc agcggcgtgc
     1201 gggcatgctc tacgcgcggc tgcggtacgg gtgtgtggag gacttcctgg caggcggcct
     1261 gcacgagttc ctgtccgact tcatggatcg catctacgaa atcggcgcag ggatcaaccg
     1321 cgacttcctg gcgcccgtcg agggtctgca atgacctact gcgtggccat gcgtctggac
     1381 gcagggctgg tcttcctgtc ggattcgcgg accaatgcgg gggtggacca gatcaacacc
     1441 ttccgcaaga tgcaggtctt cgaaaagccc ggggagcgga tgctggtcct gctgtccgcg
     1501 ggcaacctgt ccatcagcca gtcggtggtc aacctgctgc gggagcggct ggagcagtcg
     1561 cgggggccga acctccacac ggtcaagaac atgttcgagg ccgcccgcca cgtgggcgac
     1621 tgcctgcgcg agatccacgc ccgcgacgcg caggcgctgc gggacttcgg catcgacttc
     1681 aacgcctcgc tgattctcgg cggccagatc gccggcgagg agccgcgcct gttccagatc
     1741 tacgcggtgg ggaatttcat cgaggcgacc cgcgagacgc cttacttcca gatcggcgag
     1801 tcgaaatacg gcaagccgat cctggaccgg gtcatcacca gcgaaacgcc catcgaccag
     1861 gcggccaagt gcgcgctgat ctcgatggat tccaccatca agtccaacct gacggtgggg
     1921 ctcccgctcg acatgctgat ctatgaacgg gatgcgctga aggttggcaa gcaggtgacg
     1981 atcactgcgc agaacgcgta tttcaacgac ctgcggaacc gttggggcga gtgcctgcgc
     2041 aacgccttcg aggagctccc cgcgccggac tgggacgcgt ccttctagaa cgacgagccg
     2101 ggttcctgca gaaaggcgtc ttcggccgcg gtcgaggccc gccccagcac cgcattgcga
     2161 tgcggaaagc ggccccagcg ggccacaatg tcccgatggc ggcgggcata gtcggcgtac
     2221 ccgtcgaaga tcggctggag cggcacggcc acctcggccg cgagcgcttc gaacagggtg
     2281 accgacaggg cctggtcgtc cgcgtcttca gagtgttcca gaggcaggta gaagaacacc
     2341 cgctcgatgg ggcgcagggc ccggtcttgg ccgtgggcga gtccttcgcg gcacaggcgg
     2401 cgggccgtcg catcgaacgc gaatgcctgc gccgtgccgc ggaagatgtt gcgcgggaac
     2461 tggtcggtga cgaggatcag cgccagacgg ccggccggtg tggccgccca cccgtccagg
     2521 cgccccgcca gggcatcgtc cggcaggtga ccgtagcgac gggccatctc cgcatcgacg
     2581 gcgccgtcct tgccccacca caggccggcc tggcgggcgg cggtgacggc atcgtccgga
     2641 tcggttccga accagaagtg gacaagggat tgcgggtttt ccatggcgcg ctcctggggg
     2701 cggaggggcc atgatgccgc ggatttgcgc ccgcgtcatg ggggcgacgt tcaggtgacc
     2761 ggatcgacgg ccccggccgc cgtgcgctgg cgcagccaga tggcaaattc ccgcggaggg
     2821 aggggcgcgc tgagcagata gccctgcatc tcgtcgcacc cctgctgctg caggaagtcg
     2881 gcctgggcct gggtctccac gccttctgcg acgacgcgga tcttcaggtt ggccgacagg
     2941 ccgatgatgg cggtggcgat ggcactgtcc tcgggatcct ccggcgtgtc cttgacgaag
     3001 gagcggtcga tcttgagggc gtgaacgttg aagcgcttga gataggcgag ggacgagtgg
     3061 ccggtgccga agtcgtcgat ggccagctgg atgcccatgg tacccaggcg cgagaggatg
     3121 tcgcggctct gctcgctgtc ctccatcagc atgctctcgg tcagctccac ctccaggtcg
     3181 gccggcgcga ggccgtgttc ggcaagcagg ccggcgatct gttcgggcag gttgggctga
     3241 cggaactggc gggccgacac attcacggcg aggcgcagag gcggcagccc ggcagtcttc
     3301 caggctgcga tctgggcaca ggcggtgcgc agcacccagg tgccgacctg gttgatcagc
     3361 ccgatgtcct cgagaatcgg aatgaagcgg tccggcccga tgcgctgtcc cgaggacggt
     3421 gtccatcgca gcaaggcctc cacgccggtg acgcggccac tgcgcaggca ggccttgggc
     3481 tggtaatgga ggtggaactc gcgccgctcc agggcgtgcc gcaggctggc ctccagggca
     3541 tggcgctcgg ccatctcggt attgagttcg tcgttgtaga agaaataggt gttgcgcccc
     3601 agttccttgg cccggtacat ggccatgtca gcctgcttga cgagcccgtc gagatcttcg
     3661 gcttcgtggg gatagagggt gacgccgatg ctggtggaga tgtacaggcg ctcgccatgg
     3721 atctcgaagg gctgggcgat ggcggccagg atgcgggtgg cgacctgggc ggcggcggcg
     3781 gggctggtga tgtcctccac cagcaccgtg aattcgtcgc cccccaggcg tgacacggtg
     3841 atgctttcgg tggattcgtc cagcgtggcg cggctgacgg tgtcggtgct gcgcaggttg
     3901 gcgatgaggg cgttggccac gtgcctgagg agctgatcgc ctgcctcgtg gccgagggtg
     3961 tcgttgatgt tcttgaagcg gtccaggtcg aggaacatca gggccagctg acggccattg
     4021 cgccgggtac ggcccatggc ccgttccagg cgctcgcgga acaggctgcg attgggcagg
     4081 ccggtcaggc tgtcgtagtt ggccaggtag gacaggcgcc gctcggcctg cttgcgttcg
     4141 gtgatgtcgc gcaggatggc gatgaagtgg gggcggtccg ccagacgggc ctcattgacg
     4201 ctcatctcca ccgggaaggc ttcgcccgag gcgcggaacg ccgtggtctc cccttgcccc
     4261 gtggtgaagg cggtaccctg ctcctcgccg ctgaacgggc gcagcagatc ggtgatgaac
     4321 atgcccggca ggtgctcgag gggccggccg aacatgcgcg ccacggccgg gttgcaggag
     4381 gtgatgcgcc tgtccggcgt gaacagggcg atcccttcca ccgcgttctg aaagacgacg
     4441 cgcagctgtt cctcggcctc ttcgcgcgcc ttgctctctt cccgctgcag ggcggcgacg
     4501 gcgcgacgca gggcccgcag gggcaaaacg cgcagggagc cgtagatggt gaagcccagg
     4561 gccacgccca gcagggcgac aagaccggtg cggaccagcg tggggcggag ggagcgggtg
     4621 atgatcacct cgccgaccgc tccgtcgggg ccccgcagcg ccgccgcccg ctcgacgacc
     4681 agtctgccga ggggcgcgcc ggtggtggcg atcggctgct gggctgcatc cacgatgcgg
     4741 cgctgctccg ggacatcgct gggggcgagg tcggtgtcca gcaggccgcc gacttcctgg
     4801 tgccatgcgt cggggctggc cgcgatcagc tgggtgacga aggcggcgtg cagccgagcg
     4861 gccatggccg catcgcgcag ttcggcctcg cgggcaagca ggaagtatcc ggtcggcgcg
     4921 gcaatggcga ccaacagcgc ggtgacggtg gcgatccagg tgatgaccgg gacgagccgg
     4981 gcttcgttct gccagacccg ggtcctggcg gggcctgcgg tattcatcgg gtgttaccgt
     5041 tcgatcacct tgacggtctt gtccacctgg gaggcgcgga tgtagccaat ggcgttgcgg
     5101 ttgctggcga cgatgtgctt catctcgctg tcttcctggg tgacgcgggg cggcatggct
     5161 ttgccggtga agatgttctg agaccagatc gcgcgcatcg cggccgggct cttgccgatc
     5221 accgcggtgt agaaggtctt gcgcagttca ctgtcctggc cgtaatcgag caggaagatc
     5281 ggcgatccgt ccggccagcc cttgaccgcg ccggtataca ccttgacgac gtaggcgcgg
     5341 tcgatgacgt tcggattctc cgggttgatg atgacaacca tctcgtcggc gctccggacg
     5401 ctgaaggggg cgccgagcag gaccagaagg gtggcggcgc ggcaaaggga ctgccggacg
     5461 cgcgaggagg agaacgggag ggacatcaga acaccctgtc gtagctgatg gtgaggagtc
     5521 gggcgtcgcc gtggaggctg ccgaaacccg gttcggtctt gtcggtccag acgtctgcct
     5581 ggaccttgag ggcgctctct tcggtcaggt cgtaccgcag caccgccgac caggtgccat
     5641 gccgctctgg cacgcccgcc gtgtcgagct gctggcggta gtccgatcgg gacagcagcg
     5701 gttgccacgg gccatgacgg tagccgacgg cgatgaggtt ggcgtaatcc ttgccgtaat
     5761 cctgcgtgcg gttgatgtac aggaattcgc cccgggcaaa ccagttgccg ctgtcgacgc
     5821 ccatggacag gccctggatg acctgtcgct tgcggtctga aaaggccgtc tcgcccaggg
     5881 acaccacctt gttctgggtg taggactgga tgcgcaccgc ccgcagcgtc agccagtcgc
     5941 ggctgagggt gacttcggtg ccgaggatgt cggaccagcg cacgtcggtg cgcgagaact
     6001 tgccgttgta gatctgccag tagccgctgt cgtggcgcgt ctcgttgccg ccgaacacct
     6061 gaaccgcgct gccccaggcg ccccactgct cgcgccagac caggctggcg ccgtcatagt
     6121 tgacgatctc ccagccgtac agctgcggtg ggagatgcac ccagggcagc gcgaagccca
     6181 catcctgggc ttcggaatag ctgaacaggg gcaggcgctt tcggccgatc tgggcggtga
     6241 ggttgccact cagctccgcc gtggcataga gccattccag gtcggcagcg ccattggccg
     6301 caccgcgggc gacggcctgg ccggtcaggg caaagcgccc ctgggcggca ctgaccgtgc
     6361 cctgcaggcc cagtcgcgaa tccggtcccg cccgccactg cccggattcg tagacgccgc
     6421 gctgggcgta gtcggagatg aagcaggggc agcggaagcc ctgatcgacc gaggcgtcgt
     6481 gggtgccact gaggacgcgg ccggccgcga gggtcatgaa gccggtgccg gtgaagtcga
     6541 ggccgccggc ttcgagggcc agggccggga gggggaggct caggacgcct aaagccgtgg
     6601 cgcgcgcaag ttgccggagg tgcagcacgg ggaaactcca gatttttgtt gttgtggaat
     6661 gcccgggctg gacagggggg acgctgcctg accctggggc tcggtaacct ggatttctta
     6721 cggctcggcg cagagattct tgaccgatga agtgtgaatt tgtgcgttgc agcgacgtat
     6781 cctggacggg gccgcgcgcc cagatgtggc cgcaggactt tgcgtcccgc gggatgaggt
     6841 gcaaggtctc agcgcttgat ccgggcgatc aggaagggct gaagctgacc ggccatgtcg
     6901 gcggcggccg cggcaaagcg ggtcgggtcg acggtggttt tatagctgtt gtcggcgatg
     6961 gtgccggtga ggctcttggc catgctgccc aagagtcctc cgatgcctgc cttggcgtcc
     7021 cgctctgtgg tggcattctg aacgccgccg cccgccagga agtcggcttc gccgggaatc
     7081 gtctcgtcga gccgtacgac cacgtcgccg tcgtaggggc ggtagccgcc ggtgatggcg
     7141 ttgatgcgct tcgattcgcg ggcatagccg gcatcaccgt cgggcagccg cacggccagg
     7201 cgcgtctcct cgtcgcgcag gtacatctgg gcggtggcgc ccgtacggga ggtggccgtt
     7261 gtcgagaagt gggccgtgct gatggcgtcc cagtcggtgg aggcggtggc gttgccgaag
     7321 ccgatgaggt accagacctt cacgacatgg gcgttgagcg ccttggcaag ctgtacttcc
     7381 agcccggccg cgtagctgct gtttcctgca aatctggcgc ggtcgctcca cccgagggcg
     7441 ggtgcctgca gttcgccctt ttcctggcga gtgttggcga aggtgccctg ttcggacagg
     7501 ggcggaacat agggtttgag cgccgtcggg ccgacgatca ggctgtggcc ttccttgttg
     7561 ccaaagtggt agccctgcgg ccagcccatg gtctcgcgca atttctgata ggccggctgg
     7621 gcgaggacct gcgcttccgg cacgacctcg aatccggccg cggtgagatc ggtgacgagc
     7681 tttttgtaaa cctgattggc cgcttgttcg agttgtcgtg ggtcaaagcc cgcaagttcg
     7741 ttgtccgaaa aggagtctcc gcgcttgagg tacatcttgg acaggcgccc ggtgatgtgt
     7801 tcggcgaaga ccttggtctg gaattccagg attacgttgg tgatcgccac ccgcctggtc
     7861 tggccgatgc gctcggcggt gctgtcggtg aattccacct tgggcgagcc gctcagacga
     7921 gggccctcgt cggccgtcgc tgcaggggca gacaggatgc ctgccgagaa catcagcgcg
     7981 caaaaggcgc ccttgccagt gatcatgtcg gtctccggtg tcgagtgtgc agtgatttca
     8041 ctctcgcgca cggagtccct gaccacaagg gtgccatttg ctacagggcg ggacaccgat
     8101 cacgctgacc tgtagatggg gggccgaccg cgggtcggtg ggcgcgccgc gcggtcgacg
     8161 gggtccggtg cggacctgcg cctggctcag cgaatcaccg gctgtccggg gcggcgttcc
     8221 ttcagcgcga ggcggggcgt ggcagatgcc tgcaggtcga cctcgtcccc gacgatgctg
     8281 gctgcttcct ggagcaggac gtccttgcgc tccttgcgcg ccttctcggc cgcgaggtcc
     8341 gcggcgaggc tgcgctcgcc ggcctgcagt ccgtcgtccc gcaaattggc cagcttgtcc
     8401 tttgtgccgg tcttttcgcg ggccttgagg cgggcctcct gggtgtcccg ctctttgcgc
     8461 cgctcggcct cgttgaggga gatctgtttg ctgtcccgga gccggcggaa ctcggccacg
     8521 tcttccagga gatattgaaa atccttgtcg cgggccacgc gggcctgatg gcggcgctgc
     8581 agatcgggca ccaggggcac cacattgccg gccggcgtga acggggcggc gtcgatcttg
     8641 acccacggca gggcattgtc gaagctggat tcgccatagc tctcgtcgtc gctgacggac
     8701 ggcaggcgga tgtccggcgt gacgccgcgc aactgggtgg ttccgccgtt cacgcggaag
     8761 aactgggcga cggtcatctt cagctcgccg aagcggggct tgtcgttgcg gaccacctcg
     8821 tcgaggttga ccagggtctg caccgtgccc ttgccgaagc tgggctcacc gaggatggcg
     8881 gcgcggccgt agtcctgcag ggcggcggcg aagatctcgg aggccgaggc cgagccgcgg
     8941 ttgatgagca cacccatggg gccgtcccag gccacgccgg cgcgctcatc gctttccacg
     9001 ccgatgcgtc cgtcggcgcg acgctgctgc accacggggc ccttgtcgat gaacaggccg
     9061 gtcagttcaa cggcttcccg cagggagccg ccgccgttgt tgcgcaggtc gacgagcacg
     9121 ccatccacct tgtccttgcg cagttcgtcg aggaggcggg ccacgtcgcg gctggcgctg
     9181 cggtagtcct tgtcgccgcg gcggcggccc tcgaagtcct catagaaggc gggcagggtg
     9241 atgacgccga ccttgcgcgc accggcacct tcgccgactt tgaggatgga tttcttggcg
     9301 gcctgttctt cgagcttgat cttgtcgcgc accagcacga cctgcttgtg gcgaccatcc
     9361 gggccggcgt cggcgggcag gatgtcgaga cggaccttgg tgtccttctc gccgcggatc
     9421 agcttgacgg cctcgtccac gcgggtgccc agcaggtcca cgagtgcgcc ggattcgccc
     9481 tggccgacgc cgacaatgcg gtcgccgggg gcgagctggc cggagcgggc ggcggggccg
     9541 ccggggacca gctcacggat ggtgatgtac tcgtcccgct cctggagcat ggcgccgatg
     9601 cccatcaggg acaggcgcat gccgatgtcg aagtcttccg aggcggcgcg acccaggtag
     9661 ttggtgtgcg gctcgatcga catggcgtag gcgttcatga agatctggaa gacgtcatcg
     9721 gccttgttgc gaccggcacg ggcaagggag ttgtcgtagc gcttctcgag ggtctcgcgg
     9781 atggccttgt catccttgcc ggccagacgc aggcgcagcc agtcgttctt gacgcgcttg
     9841 cgccagaggt cgcgggcctc ggcgtcgtcc ttgggccagg gcgccttgtc gcggctgtag
     9901 ccgtagctct ccttcacatt gaagtcgaag ctgccggcga gcacttcccg cgcctgggtg
     9961 aggcgctcga ggacgcgctg ctcgaagcgc tggaagatgg cgaaggggac gcccaggttg
    10021 ccctgcagga tggcgtcgtc cagcgtggtg cgggcgtcgg cgaaggcgtc gatgtcggcc
    10081 tgggtgaaga acaggcgctc cgggtcgagg gacttgaagt agcgctcgaa gatcttttcc
    10141 gacagggcgt cgtcgagggg aaccgccttg taatggtagc gggcgaggag ctgggagctc
    10201 aggtaggcgg tctgggcgtg gtcctgggtc ggcgcgaggc ggttgggcgg cgtcgccgtt
    10261 tcaagggcgg ccgcggcagt ggcgaaggcg atcgccgcca ggctccaggt caacttcttg
    10321 ttcacacaaa tctcctgctc gtcggcctgt ggggcgatgt cggcgtcaac gggtgcgcgt
    10381 catcagaccg ctttatcggc cgtcgtggag cattctttca tgttcgtgaa caatgggcca
    10441 cccgcggcga cggacgggcg gtttgcgccg ccgcggggtc aggccgcggg cagggacttg
    10501 atccaggcgc gctgggcttc ctggcgacgc ttgtcggcga ggcgctcgat gatccgcggg
    10561 cagacctctt cgaagggcat gaggccgctc gggaagatct cgtcgcagcg caggatgtgc
    10621 aggccgatgg gggattcgag cacctccgac acggcgccga gggcgagggc gaaggcggcg
    10681 ggttcgagct ccgggtagag ctgcttgcgc ttgaccacgc cgagcagtcc gccgtccatg
    10741 gcggtggggc actgggagtg gcgcagggcc gccttggcaa aggcttcggc gctgcccagg
    10801 ccgcgcaggc cgtccagggt cttgtgggcg cgggccttct cgttaccgcc gttgaaagtg
    10861 atgaggatgt ggcgcaggcg gcgtgcctcg ggtcgatcga aggcttcggg gtggaggcgg
    10921 tagtagattt cggcatccac cgcgctgacc ggctcggcgg cggaggccac cttctcgagg
    10981 acaccttcca cccgcaggtc acgctcgacg gccttgtcca gttcaccctc ggagaggccg
    11041 atgcgctcca gatcgcggat gaggtcgtcc tcgctgggat agcggcctct gatctcgtcc
    11101 acgcgctggc gcagggtggc tgccgggatg gcgacctgag ccgcttcggc actggcgagg
    11161 atgcgcagct cgatgcggtc ctgcttgcgg gcgatgtcgc cgacgcggat ccgctcgggc
    11221 tcggacaggg cgtcgggcgc cttctggaac atctcccagg cgagtttcag ttcgagatag
    11281 ccgtgcagca tggggttcat tcctccgcgg gggcctccgc cgttgcggat tcgagcaggg
    11341 attcgggaat ctgcaggatg cggccgggca ggctgtcgaa gtggatgtgg taggccacgc
    11401 cgccgggggc gtcacggatc accttgatca cttcgccggt ggcgccggcc gtgatgagca
    11461 tctcgccgcc gacggagagg cccttgaccg cccggacctt gtcgcggaac tcgaagcgcg
    11521 acggggtcca gggctcgtcg gcgccgatga gctcttcctc gcggcagccg acgatcttgc
    11581 cttcgtccag gaagttcacc gagtagatga cctggtcctg gaggaaggtg cccacgtcca
    11641 ccacgtagcc gatgctgccc cggcgcacca ggaaggcgcc ggggtcgagg cccggatagg
    11701 tgccgtcgtt gcgcacgttg cgggtcaggc ggacggcttc gccgtactcc cagcgggcgt
    11761 ccatcatgac tggaagacct tgtcgatgga tacgaaggag gtcatgccgc cttccggcgt
    11821 gctggtgttc tggaacaccg cgaagacctt ctcggtgagg gcgtcggagt tcacgaagtc
    11881 ggtgtaggcg cgggtcagcc actggcgata gatgtcggta cgggcggcct cgtcttcggg
    11941 catctccttg gcctgcttgg ccaggtagtc gtggaagcgc tgcaggatgt gcaggcggtt
    12001 cacatgcacg acggactggt cgtagggcac gccgaagtag ttcaggaaat cttcggcgga
    12061 caccagttct tccatggctt cgtcgagggt cagggtatcg tccatggggg cctcctcagg
    12121 ctgcgacaag ggatagatcg gattcgtcgg ggttttcgaa cgttgcaatg acttccagca
    12181 gcttgctggc gccgagctgg tcgcggctat ccaccgcggc cagtacgtgc aggcgggcgc
    12241 gggattcctc gatgcgtccc aggcgcagca gcaccacgct ggcggccttg agggccagca
    12301 ggtagaagcg cagcaggccg aaggagatcg ccgcggcgat ggccagcgag gattcgtcga
    12361 gggattccca gtccggcagg ctgagctgac ggcccgagag gtacatggcc cgctcggcga
    12421 cccgcagggc ttcctccagg cggtgctggt agaagtagaa gcggtacagg ccgacaagga
    12481 cggtcaggtt gtccggcacc atcaggttgg cccgcagcag ggcaatctcc gcgtcgggct
    12541 ggccgtattc gcgggaggcc tgggcgagca tctcttcggc ttcgggaggc agatcctcct
    12601 cgaagtagag cttgcactcg gaaaagtcga gcagatccat ggagacctca gtgcatggac
    12661 tcgtgctcgc agcacaagtc gccggcggaa caggtctggc actgtccgct gtgatcgggg
    12721 ggaggcgcct cgtggcgcag ttcggccagt tccttctcca gggtttcgat gcgttcgatc
    12781 aggcaggcga tggacttggc caccggatcg ggcagcacgt tgtgattcag atccacgccg
    12841 tcctcgctgc gggcatggcg ggtatcgacg atgcggccgg ggacgcccac cacggtgcgg
    12901 tcggccggga cgtccttcac caccaccgag ttggcgccga cgcgcacgcg ttcgccgatg
    12961 acgatggggc cgaggatctt ggcgccggcc cccaccacca cgccgctggc cagggtgggg
    13021 tgacgcttgc ccttgttcca tgaggtgccg cccagggtca cgccgtggta cagggtgacg
    13081 tcgtcgccca cctcggcggt ctcgccgatc accacgcagg cgccgtggtc gatgaagaag
    13141 cggtggccaa tggtggcgcc ggggtggatg tccacgttcg tcagcgtacg gctgagaaag
    13201 gacaggaagc gcgccgggta gcgccagccg gcacgccaca ggcggtgcga cacgcggtgc
    13261 agcaggatgg cgtgcacgcc ggggtaggtg gtgagcactt ccagcgtgga gcgggcggcc
    13321 ggatcgcgct ggaagacgca ggccaggtct tccttcagga gatccaggag gcccggctgg
    13381 gccggcgtcg gagcgatgat ggtcatggcg tcaggcatgg aagtgactcc tttcggaggc
    13441 cagcaggcgg cgcaggtcgg ccgcttcggg ggggtgcttg tgatcggcca cgaaggcccg
    13501 cacgcgggtg agcagggcct gggcttcgcg ctcgtccagg gggatgccca gctcggcaaa
    13561 gaccacctgc acggcgcgcg agccagagtg cttgccgagg acgatgcggt gggagcggcc
    13621 cacctcgcgg gggtcgaagc cctggtagtt gtccggatgc ttgagcaggc catccacgtg
    13681 gatgccggcc tcgtggcaga aggcgccggg tcccaccacg ctcttctgcc agggtacggc
    13741 gcggccggag gcactggcca cctgggcgga gagggcgggg aagcctttga ggtcgatgcc
    13801 ggtctcgatg ttgtggagct tgcgcaggcc cagcaccact tcctccagcg gggcgttgcc
    13861 ggcgcgctcg ccgaggccgt tcaccgtggt gttggcgtgg gtggcgccgg ccaggcaggc
    13921 ggcgagggtg ttggcggtgg cgagacccag gtcgtcgtgg gcgtggatct cgatctccag
    13981 atcggtggcc cggcgcaggg tggagatgcg ctcataggtg ccgaaggggt cgaggatacc
    14041 cagggtgtcg gcaaagcgca ggcggcgggc gccggcccgt tcggccgtct cggccagggc
    14101 acagaggaaa tccaggtcgg cgcgggaggc gtcctcgcag ccgacgccca cttccaggcc
    14161 gaggctcagg gcggcgccga tgtagcgggg cagctcccgc agcagccagg cccggtcctg
    14221 cttgagcttg taggccagat gctggtcgga ggcgggtacg gagatgtcca ccagatgggc
    14281 gcccaggtcg gcgcaggcgg cgatgtcgtc cgggtgcatg cggctccaca ccatcaggcg
    14341 cggtgccaga tccagggcag cgacggcgcg gatggagtcg cgctcctcgg cgcccatggc
    14401 ggggatgccg acttccagtt cgggcacgcc gacggcggcc agctggcggg cgatttccag
    14461 cttctcgtcc agcgagaacg cgaccccggc cgactgctcg ccgtcgcgca gggtggtgtc
    14521 gttgatcgtg acgaaggaag tctggggcat ggcaatctcc ttgccatggc ttcagcaagg
    14581 acggtgccag ctttcaagtg cttgattcac tgaaaaaatg cggaaatgtg tggcgggctg
    14641 tggggttcaa tgtcgagaac ccgacaatgg cgctcgaaca ccgtaggggc gtcttgcctg
    14701 cagggcagac agacggtccg gtgcccgcaa ggggccccac cggcgtgaca cggttgtgga
    14761 tggctgtccg atgggcgtac ccggtgcggt gggcgctagg aatccggggc aggggcctgc
    14821 gcggtgaggc ggacgtactg tggaacggga tgggcgctca tcccggtgcg gggggccgtg
    14881 ctccgcccct cagacacccg cagggccgcc ctgggtgccg tcgggcgtta cggcacccag
    14941 ggtggccggc cgatgccggc gcgggttcag accgaatagg gccgatgcag ccagtcccgg
    15001 acgacggcca cgtccttttc gggcagatag gcgaagccgg cgagggcgtc ctccaccacc
    15061 gcctgggcaa cccagtaggg gacggccttg gtggtgagca tgtggaagta ccaggccatg
    15121 ggatagccga cggcgcggtt gaagcggatc agggcgtccc ccagggcgat gccgcgggtg
    15181 ctctcgccgg cgctgaactc cggcgggtgg ccggccaggg tggcgagggg ccgggcgtgc
    15241 aggaccggct gctggtaacg gtcctggtgt tcctgggtga ggatcaccca gtggttgctg
    15301 aaagcgctgg cgatgccggc gctgctccgg tcccagtcgg tcagcagtcg atggatcggt
    15361 gccggttcgg gcttctggac aagcatgcgg cgcaggaagt cacctacgtg ctggagccgg
    15421 cggaaggtat aggtgggcag cccgatgggc ggcaaggcgg cgctgcccgc ggcgcggggc
    15481 gggtcgacca ggtcctcgac gctgtccggc gtggtggcga agagcgcgtg ggcgcggacc
    15541 tcctgcaggc attcgatctc gagctgaata aagtctccgg cgccttcgcc ggagggggcc
    15601 acgaggtagt gggtctgacc gaccaggcgc gatgcgtaat gggcctggcc agcgtagtcg
    15661 tcgaggaggg cctcaagctc gccaccgcgg gcgtccagtt ccgcatcgac ccaggcttcc
    15721 agcgaatcgc tcacgcgctc gccggcgcca tcgtagacgc cccccaggcg ataccagccg
    15781 ccgcgcgaga aggcgtgacg gaacttccat tcgggaacga cgctgccaag ggcggcgacg
    15841 aggcccgccg gaccggtgtt gacggccgtg tgggtgcagg tttcgacgat ggcgggggcg
    15901 agggacttgc agtactcggg ccaggcctgg gggtctttca tggcaatttc ctcgagggat
    15961 caggcggctt gggaatccgc caaacgggca agagccgctt cccggacctc cagttcggga
    16021 tccgaagtga gcggcggcag aaattcgagg ggcgcattac ccacggcggc cagacgaacc
    16081 caccattcgc tgtcctgcag cagggcggcc gcgaggccgg gcagcgcacg ttcggcgacg
    16141 atggcgcgga cttccggctc gggatcgtcg gcgagcatgt gcagggcgaa gggcggcagg
    16201 cgcgatgcaa ccaccttgcg gacctcccgc tcggcgtcct tggccagcct ggagagctgg
    16261 ccgtggggca ggcggcgggc gacggtggcg cggatgaggt agtccgggtc ctggaccatg
    16321 cgggtcagga gcggctcggg gaggcgcgag gcgaccgaca ggcggacttc ccggtccggg
    16381 tcgcccagca tcgagtccag ttcgtcggcg ggaatgcgcg tggccacgac gcggcggacg
    16441 acttcgtccg ggtcgctgcg caggccgcgg atggcctcga ccggtgcgta gcgcgcggcg
    16501 atggcgcgcc gttcccagaa caggtcggcc aggtaggcgc cggagagctc cgggttggcc
    16561 cgcaggaagc ggtcgatctg gcggccgctg tgggcgcgga tgcaggcgtc ccccggcacg
    16621 caacggccgg cattcaggaa gtccgggaag tagctgcagc ccgaacacag gcccaggcga
    16681 ccgatgccgg cgtcggccgc gcccttgtcc tcacgcttca tcggccttgg cggcaaccag
    16741 gatgccggtg gcaagggcgg catcattggt cacttcgagg cagtgtccgg tagcgccgga
    16801 gatgtagtag agggcgtggc cgtcggtttc gagggcagcg cggtagcggg gcgacacgtc
    16861 gtcctcgctg cattcggtga aatgcaggtc gggaaaacgg gcacgcaggc tggcgatggc
    16921 ggcgtcggcg cgcacggcgt cggccaccag ggtgagttgc tccggggtga tcacgatcag
    16981 tcctccgctt cgagttcctc ttcgaaacgt gccagcgacg cggccggcac gcccatgatc
    17041 tgggccagcc aaggcggcgg gctgctgaga cgtccctgca gttcggtcag gatctcccgc
    17101 gccgggccgc cggtaggctt cttgactggg tggacaccgg cccgcacgac cttggcggcg
    17161 gcgggaccac cgatggactg gacgtacacg atctgacagt cgttgatgag ttcggagcgg
    17221 gcggcgttgc ggtcctcggc cgcatcggcc tcgacggtgc tgcgcacgcc gacgaggcgg
    17281 atcgcatcga ggccgacctg gtagatcagg aagcgctcgc aggagccaaa gtgtccgtcc
    17341 agattctcgc cccggttggc ggcgatggct acccggatgg agccgggcat gtcaccgtcg
    17401 gaataggctt cgacctcggg aaagtcgccg gcgcccacgc cctggcccca caggaggcgg
    17461 atcgcctcct tgagggtgtc ggcatccacg cctacgtggc agccctgttc ggcgaaatcg
    17521 cctgccagga gcgcccgcag gtcctcgacg gtgagggtgc cgagcttggc ttcggtgagg
    17581 ggggcttcga gcttgtcgat gagggcgccc acgaaggcgc gtacatcaag gccggccaga
    17641 cagcgggccg ccagggcgat gcgcagggcg gcctcgcggg aggcgatggg ggagtcagac
    17701 atgacagggc tccaggaaag tccagagtgg aacgccggcc ggggccggcg ctcggcactg
    17761 ggcttgcctc aggccgggct gatgcagccg gacgggcaga cgtctacgca caggggcgag
    17821 tcgttgtggc cgtcacactc ggtacaggtg ctggcgtcga tcttgtaaaa gatcttgccc
    17881 gagctgatcg acttggtcgg gcagacgggc ttgcagtcgc cgcaggcggt acacatgtcg
    17941 ggatcgattt tcattgccat gatgaagtcc tccagatcag tcctcggcgg cgccgagacg
    18001 tttggcacgc agggagatgg gcaggcgcgc gggtttggcg atggggtcga tgtagtacga
    18061 gccgccattg tccagcttca gttcgccacc ccatttttcc gggctgtcga attcgatgga
    18121 ctcgatggag gcttccaggt cgcgcttcgg gagatagaag gtgagcccct tctcgccctg
    18181 acggatcatg atgttggcca tttacgaacg ctcctgcgga aacggcgggg ccttgcggcc
    18241 ccgccggtct ggccgtgatt agcggaccag gtcgaagttg tagtcggtgg tgcccatgcc
    18301 gatggtttcc ttgtcgagct gctcaaggac cgcattggtg agctgggtga ccacggacat
    18361 catgccttcg tagcccaggg tggtggcccg gtgcatgtgg tgacggtcga agatcgggaa
    18421 gccgatccgg atcagcggca cttcaaactc ttcacccttg tgacgggtgt cgcgctggat
    18481 gtacttgccg taggagttgc cgatcaggaa gtcgggcttg tcggtgaagc acaggctgcg
    18541 catgtgccac aggtcgttgc cgacgtagac cttgccgagc ttgccgaagg ggctggcgtc
    18601 cagcagcttc tgcacggcct tgccccagcg cttgttggcg ttgtgggaca ggatgtgggt
    18661 cggttcggca cccacttcca gcaggatctt gaccatgccc atgacgaagt ccgggtcgcc
    18721 gtagagggcg aacttcttgc cgtgcagcca ggcgtggctg tcggagatca ggtccatgca
    18781 gcggccgcgt tccagttcca gcgaggcggg gatggcctgg ccggtcagct cggagacctt
    18841 catcaggaac tcgtcggtcc actcgacacc catggggatg ttgagcttgg gcacgtcgtg
    18901 ggcccaggtg gtttcgacga acttcttggt cttttcgagc tgccagggct ggagcaggac
    18961 ggtggtgatc gcgttcggcg cgtccttgac ctcgtccatg gtggtgccgc ccgcgtacat
    19021 gcggaattca ccgtcggccg gggtgtcgaa cacgtcggtc ggatcggaca gcatggtgta
    19081 gccgacgccc atttcggtga gcatgcgctt cacgacgcgg tagttgccca ggtaggtctc
    19141 gaaacccggc accaggttga tggtctgctt ggtgccggcc ttcttgccct ccatgttgtt
    19201 gagggtgaag tagcgcagga tgccttcttc catgttgtcc cagccggtgg tgtgcgaacc
    19261 gacgaaggac ggggtgtggg cgaaggggac cgggtactcc tggggcacgt ggccggcctt
    19321 cttggcgttg ttgatgaagg cgttcaggtc gtcaccgatg acttcggcca tgcaggtggt
    19381 ggagacggcg atcatgtccg gcttgtagat ggccttggcg ttttccaggc cgtcgaacat
    19441 gttcttctgg ccgccgaaca ccgcggcatc ttccgtcatg gagtcggaca cgcaggagca
    19501 cggttccttg aagtgacggt tgaagtaggt gcggaagtag gcgacgcagc cttgcgaacc
    19561 atgcacgtag ggcagggtct tgtggaagcc ggaggcacac agcacggcgc cgaggggctg
    19621 gcaggccttg gccggatcga cggtcagggc ttcgcgctgg aagttgaggt cctggtattc
    19681 cttggtggtg gtccagagga aggtttccca gaccttgctc ggctcatgac gctcttcgaa
    19741 ttgctcgcgc ttctcggcga ggttcttctt gtagtcgtca tcacggaaca gcgggtagca
    19801 cggcttgatt ttgtcagcag tttgcattgc tttctccttg cccgtaccgc tcatctgcag
    19861 tgccgggcgc aaggttgaag atccggtgtg ccgggccccg gccggtgaag gacgctcggt
    19921 ccgcaatcac cggcgcaggg cgccgtcgtc cgattcctgg gctcaggcgg ccttcttgac
    19981 gtcctcttcc gcagccttct tccagggcgg ggtgaggttc ttgaaggtcg ggttggacag
    20041 ggcgatgtcc atgtcacggg cgaagatggc gaagccgtcg tagccgtggt acggaccgga
    20101 gtagtcccag gagtgcatct ggcgcagggg aatgcccatc ttctggaaga tgtacttttc
    20161 cttgatgccg gagccgacca tgtcgggctt gagacgcttg acgaactctt ccagctcgaa
    20221 gccggtgacg tcgtcgtaca gcagggtggc gtcgcccatt tccttgatgg tgcggtcgta
    20281 gtcgtcgttg tgggcgaatt cgtagcccgt gccgatgact tccatgccca gatcttcgta
    20341 ggcaccgatc acgtgacggg ggcgcaggcc gcccacgtac agcatgacct tcttgccttg
    20401 caggcgcggt ttgtacttgg cgatgatctc gtccatcatc acctggtagc gggcgatcat
    20461 ggcttcggtc ttttccttga ccgagtcatc gaagaaggca gcgatcttgc ggcaggattc
    20521 gacaatcttc gtcgggccga agaagttgta ttccatgtag ggaataccgt acttctcttc
    20581 catgtgacgg gagatgtagt tcatcgaacg gtagcagtgc agcaggttca gcttgacctt
    20641 cggggtgttc atcatctcgg cgatggtgcc gtcgccggac cactgggcca cgacacgcag
    20701 gcccatttct tcgaggatga tgcgggtggc ccaggcatcg ccgccgatgt tgtagtcgcc
    20761 gatgatggcc acatcgtagg gggtgctgtc gtaggcctgg ccgtcgcgct tgtcgaggac
    20821 ccagtcacgg atcgcgtcgt tggcgatgtg gtggccgagg gactgggaga cgccgcggaa
    20881 gccttcgcag cgcaccggca cgacgggctt ttcgatggcc acggccgcct tcttggagac
    20941 ggactcgatg tcgtcaccga tcaggccgat cgggcattcg gactgcacgg agatgccctt
    21001 gtgcagcggg aacagggttt cgatttcttc gatcagcttg gcgagcttct tgtcgccgcc
    21061 gaagacgatg tccttctcct ggaagtcgga ggtgaagttc atcgtgccga aggtgtcgac
    21121 accggtggtg cccacgtagt agttgcggcg accggcgcgg gaatactggc cacagccgac
    21181 gggaccgtgg gagatgtgga tcatgtcctt gatcggaccc cacaccacac ccttggaac
//
LOCUS       DAOQMM010000062        21207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_873_length_21207_cov_47.499433, whole genome shotgun sequence.
ACCESSION   DAOQMM010000062 DAOQMM010000000
VERSION     DAOQMM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21207
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_873_length_21207_cov_47.499433"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /gene="cysC"
                     /locus_tag="PLH85_10935"
     CDS             complement(<1..611)
                     /gene="cysC"
                     /locus_tag="PLH85_10935"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNB65126.1"
                     /translation="MRERLNGHRGRVLWFTGLSGAGKSTLAHAVEACLHEQGRRAFVL
                     DGDHLRHGLCAGLGFSSADRSENLRRAAEVARLFADAGTVTLAAFISPLREDRERVRA
                     IVGADRFVEVYVACPLAECESRDVKGLYKRARAAEIPHFTGISAPYEAPEHPDLVLDT
                     SRTPIEACVAQVLACVDRIVSLEADTFTDIGESMHPAPTDRAPL"
     gene            complement(668..3565)
                     /locus_tag="PLH85_10940"
     CDS             complement(668..3565)
                     /locus_tag="PLH85_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB65127.1"
                     /translation="MNASRKVLLVGWDAADWKIINGLVDRGLMPHTASLIERGVMGNL
                     ATLHPVLSPMLWTTIATGKRPFKHGVLGFTEPSPDGSGVQPVSQWSRKTKALWNILNQ
                     SGLKSHVIGWWPSHPVEPINGVMVSNHYHTALGPPDQPWPLAPGMVHPPRAQQALADV
                     RLNPNELLPEQVCFFVPRAAEIDQDKDRRLASVMKTLAECTSVHAAATWAMANEPWDF
                     MGVYYDAIDHFSHGFMRYHPPRRPFVPERDFELYRHVVEAAYQFHDLMLGTLLQLAPP
                     DTTVIICSDHGFHSDHRRPAQIPNEPAGPAVEHRDLGMFLIAGPGIRRDHLIHGASVL
                     DVTPTVLTLFGLPVGEDMDGKVLLDVFDRTPEVAHVPSWDAVPGDDARLSGEQQFDPL
                     AAKEVMDQLVALGYIEKLDDNREQAVAQTVRELRYNLARSYMDAGRMLDAAPLLQELY
                     AAEPDQYRFGVHLALCYRALNRVAELRPLVERMTRDRQEAAQTARAALQALCDTVRQR
                     QQAVATTTAVRAQDDPPSAADALASIDLSQLTEDERSQVDELRFKAWFSTSDLDYLMG
                     WVLGAEGRHDEALAHLLKAERADPGRPGLLVQIGEAHLAVHRWDEAEAAFRRALAADA
                     LSPYAHLGVARALLPRRARAEEAVEAALDSIRLLYQNPLAHYVLGLGLLRLRQFFMAA
                     DAMRTALALNPNFVRAHRVLARFYRLGTGEHDKARAHLEAIQHIRLGKRAASMTGQPG
                     LGEAESLAHEVDDEAGRPGLHADAVFAVRKTPDDPAEFVTVVAGLPRSGTSMMMQMLA
                     AGGLPILTDNKREADLDNPRGYFELEAATRLRTDRTWFREGRGQAVKIVAQLLPYLPG
                     DVPCRVIFMERDLDEVLKSQTVMLDNLGREGARLSDAQVQASYRQQLRRVKLWLARQS
                     QVDVLFVRHRDALADPAAVAEQVNAFLGGRLDCLAMATCVDASLYRQRATA"
     gene            complement(3643..4161)
                     /locus_tag="PLH85_10945"
     CDS             complement(3643..4161)
                     /locus_tag="PLH85_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65128.1"
                     /translation="MHRHVDSSRFGAYRIENVHLHTSSEQRQEIVQFWLRERAIPTLP
                     EAVRRAHEVCLLVRRDDGTGALAGVSTVGRMRTRSGLIYWVFRMFLRKPDRVPRLSLA
                     VANATCAHLRLRARREGDAAGMLIVTENVKLMRPGARMLFQRQGCRYVGRNARGQDVW
                     VVDFPAHATAAA"
     gene            complement(4211..5134)
                     /locus_tag="PLH85_10950"
     CDS             complement(4211..5134)
                     /locus_tag="PLH85_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB65129.1"
                     /translation="MNNALAVPPIPSTRDVFMQNSSSVNFGAPLRAKLESYEAISRVE
                     ARSGKSAREIAGYAAAAGAALAMAGNADATVIYSGVQNLVAQIDPAHQFGVSYVNGHA
                     GYSVNFQNFSVNGVPFMAGAVIAAELGTNINNNAGLAAKYIGAAVVQGSGVQFVAGSS
                     SPLGLATPVAPGAAIGAGSNQFGGAAGAVRMGVGYVNGKTVQYGNLLLGDNLFGFALP
                     NGNFGWIRLNIEDLGLNQPFSDILQNAGGISPLFNGQGFPDRITVVDWAYDDQGRTIF
                     AGTLNVPEPGALSLLAAGAAGIATMRRRKLH"
     assembly_gap    5516..5615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6282..7058)
                     /locus_tag="PLH85_10955"
     CDS             complement(6282..7058)
                     /locus_tag="PLH85_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB65130.1"
                     /translation="MSSSKIAVQRLSDTIAHELERRILEGSLKPGDRLQAERELAAEL
                     GVSRPSLREAIQKLVSKGLLHSRQGGGTFVTDRLEAGFTDPWSEMLRQHPDIQSDLLE
                     FRRMLETESAGLAARRATDGDLQRIGDAYARVEGLFAQGPSAEVFAEQVRADLAFHQA
                     IAEASHNVMIGHLTASLFRVTHDNIDRNLRHLRRLEDDWQELRRQHAAIWEGLRTRNA
                     GAAQAAVERHIAFVEESMAATARQAERERRAQTVRIRSPE"
     gene            7341..8156
                     /locus_tag="PLH85_10960"
     CDS             7341..8156
                     /locus_tag="PLH85_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB65131.1"
                     /translation="MSDTLPGGGPTPRRYPSRPAAVYLYATCLVDLFVPEAGLDAVTL
                     LEREGLRVIFPENQTCCGQPAYTSGFPDEARQVVASQLDLFPEDYPIVVPSGSCAGMM
                     RWHWRGVVGDDPALAARAAALAERTFELAEFLVHVLDFRRPDTGAPTTVTLHTSCSAR
                     REMGTLLVGRELLARLGQVTLAVHDHESECCGFGGTFSLKHPDVSTAMVGDKVNALRA
                     TGAAQMVTADCGCMLNISRRAAKEDRDAGRVGASLPAEHLASFLLRRTGGKPA"
     gene            8153..8827
                     /locus_tag="PLH85_10965"
     CDS             8153..8827
                     /locus_tag="PLH85_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNB65132.1"
                     /translation="MSARNSILARLRATRPAAADPLPDLDAHFAPRARHETVAQRASR
                     FRSGIEAFHAEVHVCADADWPELLARLCAAKGIGTLLYGRNTPAGERIDATPFGATQI
                     RPWQGRFEELKADLFHRIDAGFTTARGAIAETGSLILWPSTAEPRTLSLVPPVHFVLL
                     DARSIHATFFDALRAEQWSDRLPTNALLISGPSKTADIQQTLAYGAHGPKELVVILTH
                     AEGASA"
     gene            8824..10275
                     /locus_tag="PLH85_10970"
     CDS             8824..10275
                     /locus_tag="PLH85_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNB65133.1"
                     /translation="MTAPRPAGDARPIAIVSAEALRQRIHGAVNDPHLRSSFRGAMDF
                     LMAKRAAQFPDADELASLRSLSESIRQYSLSRLPDLLEQLEARLTANGVHVHWAETPD
                     EANAILLDIARRHAAQLIVKGKSMVSEEIEFNHAMHAAGIDALESDMGEYIVQLAGEK
                     PSHIIMPAIHQTKQQIARLFADKIPGVDYTDNVDALIQIGRRVLREKFETADIGLSGV
                     NFAVAETGTLCLVENEGNGRMCTTVPRVHVAITGIEKVVEKLEHVAPLFSILTRSATG
                     QSVSTYFNLISGPRKPDERDGPEEVHLVLLDNGRSQAYADAQLRKTLQCIRCGACMNH
                     CPVYTRIGGHAYGTTYPGPIGKIVSPHMMGLQQTHLMPSLSSLCGACGEVCPVKIPIP
                     ELLIRLRGEAQSAPQANPVMAGQGAGHDRLVSFAWRAWARVYASPLGYRTFSWLASRF
                     RALTPTRQAGWTAARLPLQPAPRRLRDMLRDRR"
     gene            10378..13152
                     /locus_tag="PLH85_10975"
     CDS             10378..13152
                     /locus_tag="PLH85_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNB65134.1"
                     /translation="MSALIDALRQRLPASRVITDELRRLAYGTDGSFYRLIPEVVTVV
                     HDEAEVRDVLDLARQHGRSVTFRAAGTSLCGQAVTDGVLVLLGEGFAACRIADDGAHV
                     AVGPAIVGAEVNRRLAPFGRKIGPDPASINAAKIGGIAANNSSGMCCGTAQNSYRTLA
                     SLRVMFADGSVLDTGDSASRAAFQKSHGALLQGLAELATAVQGDAALATRIRAKYKIK
                     NTTGYSLNALVDFADPIDILAHLMIGSEGTLGFLSEVRYHTVPEYAHKASALVFFPDM
                     ESACRAVVGLRQQPVDAVELLDRASLRAVADKPGMPPLLQTLADGATALLIETRAPDA
                     VTLDARIAAVLAELARHPLTEPAVFTTAPAEIERLWNVRKGTFPAVGAVRKPGTTVII
                     EDVAVAVEDLAACCLDLQRLFAAHGYHEAIIFGHALEGNVHFVFTQDFGIESEVARYA
                     AFMDELCTVLVLKYDASLKAEHGTGRNMAPFVELEWGSEAYALMKAIKQLFDPQGLLN
                     PGVIINDDAEAHLKHLKPMPAAGQLYAPVDRCIECGFCEPQCPSHGLTLSPRQRIVAW
                     RELSRRAAAGEAPDTLDADYTYMGLDTCAACGLCSTACPVGIETGALTRAVRGERLSG
                     VARRLGRLAAEHFGATQALARTALKAGHLAESVIGTRALARLSGGAWKRGMPHPQPAP
                     RASRSAGDPVVYFPTCAGRIFGADAPEQTLSATVIRVMERAGYAPIVPDGVDALCCGQ
                     SFASRGLADEADRKSAELEAVLRAASRDGAIPIVLDASACSLRMKTFLAGRLPVLDLV
                     EFAHDALLPRLMLQRKADPVLIHLNCSARRMGFETQLKALAAACAEQPVLPPEVKCCG
                     FGGDRGFVVPELNAHALRKLEVPAGCCGGYSSNATCEIGLTDTTGLPYRSIVHLLDEC
                     SAEAARIATC"
     gene            13211..14857
                     /gene="lldP"
                     /locus_tag="PLH85_10980"
     CDS             13211..14857
                     /gene="lldP"
                     /locus_tag="PLH85_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNB65135.1"
                     /translation="MQTWMQIYDPAGNLWLSALVAALPIIFFFIALAVLRLKGHIAGT
                     ITVGIALAVAIFFYNMPVEMAFASAGYGFLYGLWPIAWIIIAAVFLYKITVKTGQFDI
                     IRASVISITDDQRLQMLLVGFSFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
                     LIANTAPVAFGAMGIPIIVGGQVTGIDPFKIGQMAGRQLPLLSVIVPFWLVAMMDGWR
                     GIKETWPAILVAGGTFAVTQFFTANYIGPELPDITSALVSLVSLTVFLKNWKPKNIFR
                     FDTQHHAELGEDQHYSLGQIAKAWSPFVILTVMVTIWSLKPFKALFAKDGPLYSLVLQ
                     WPVPMLDKLVIKMEPIVAKATPYAAVYKLDLLSATGTAIFLSALISMLVLGLRGGEAV
                     KTFKETVLELKRPIYSIGMVLAFAFVANYSGLSATLALLLAGTGKLFPFFSPFLGWLG
                     VFLTGSDTSSNALFCSLQATTAHQVGVSDTLLVAANTTGGVTGKMISPQSIAVACAAV
                     GLVGKESDLFRFTVKHSLLFATMVGIITTAQAYLFTGMIP"
     gene            15086..15706
                     /locus_tag="PLH85_10985"
     CDS             15086..15706
                     /locus_tag="PLH85_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNB65136.1"
                     /translation="MHKKIVVSAIAAIAALSAGVAHAESPWMVRARAVNLDWDNKNAD
                     NLQNAGVGDVSAKKRWIPEVDISYFFTPNIAAELVLTYPQDVDIQTSALGKIGKVQAL
                     PPSLLVQYHFTELGAFKPYVGLGVNYTIFTKKSFDTALGTVSVDRASFGFAAQVGADY
                     KLDKNWSLNVDAKYIQMDTDVKLGGSKIGKLDLNPVTWGVGVGYRF"
     gene            15992..16840
                     /gene="fdhD"
                     /locus_tag="PLH85_10990"
     CDS             15992..16840
                     /gene="fdhD"
                     /locus_tag="PLH85_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNB65137.1"
                     /translation="MPPLPPDECPPDEPPAPQATTTLTVRRLDGPQLRATEDTVAEEV
                     PVALVYNGISHAVLLATPADLEDLALGFSLSEGILATPTELFDSETVATPHGLELRLH
                     VAAGCFADLKARRRSLAGRTGCGLCGVESLAALARAPGPVTRGAPIPASAIAAALAAL
                     PAHQPLRATTGAVHAAAWIDRRGTLLALREDVGRHNALDKLLGHLARSATDPHAGWVL
                     VSSRASYEMVQKVATVGIGSLVAVSAPTALAIRLADAAGVTLAGFARDRRLVIYTHPD
                     GLLADA"
     gene            complement(16847..17692)
                     /locus_tag="PLH85_10995"
     CDS             complement(16847..17692)
                     /locus_tag="PLH85_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase-like protein"
                     /protein_id="HNB65138.1"
                     /translation="MSSSVLTLPSPFCPSDRIGLLESPECDRSAVLRRVETGDYDKPF
                     VLDDGTTRSLHFSLALIQSTMSLKDPFALELDYTRAMMSFLLFLPRPRHLLMLGLGGG
                     SLVKYCHRHLGSARLTVVEIDPHVIAFRDEFEVPPDDGRLQVIQGDGAAWVADCSERP
                     DVILLDAFDRDGLSGSVSNETFYGQVCAALAGRGWLVANLAGDKAERVAHRDMIAAAF
                     GERVWSLTVPDGGNDIVLAARAEGFAPRWRELANQARALRARLGLDFPDFVRRLERSQ
                     RLRWT"
     gene            17827..19566
                     /locus_tag="PLH85_11000"
     CDS             17827..19566
                     /locus_tag="PLH85_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB65139.1"
                     /translation="MQRHRYLRLSDIATTSVRTVLPDSPLRDAIALMAQQHVSCVVVT
                     EGLRPVGILTERDIVRVMSNGLRPATAIGDVMSQPLLTARMDLDFPAAQNLLARQGIR
                     HLVVTDDAGQLWGIVTESDFRAHLNRDLFSRIRSIESVMDPEFTTLPPDAPLQRALDC
                     MSTGRCDHVVVTRQGSPVGILTERDVPRLMLRGVNPSTTALDQVMSSPVQSIRTHDTL
                     ATATDRMDAAGIRHMVALDEQDRLAGVVSQHRMLERLGATLLDEMRSRLQDRLDVILD
                     ATGVGTWEYDYAAGRLTRNAALWKLLGVDAPSGRDETLDAAFARVHPDDLPRLRTIIE
                     AAREQRNGNDEPFSYEYRVLRPDGSWLWLRTIGRNVDRDDDGLALGAAGITIDISGEK
                     AATSRATESEHRFRRLMERLPIPVMHIARGGRCLFLNDHFQQIFGYTIDDLPHSDAWR
                     QRAFPDRDLRARADAAWREAFLTARRHGQAIAPQEFRVVCKDGSVRIVDASGIVLGDD
                     FLATFVDVTERKSVQEQLSLRLAELNRWYDAMLGREGRVLELKREVNALLRATGQPIR
                     YPSADQPSEGRPA"
     gene            19635..>21207
                     /locus_tag="PLH85_11005"
     CDS             19635..>21207
                     /locus_tag="PLH85_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB65140.1"
                     /translation="MDAASSAPALSALAWIAVGALGAVAATVALRRRHGNADADARTS
                     TDAESLRLQTLLHTIPDLVWLKDPDGIYLACNRAFQTYFNAPEADILGRSDADFVGAE
                     HAARVRDDDAAAVAAGTPTATERWSIHRATGAQVAHEITRTPMFAADGSLIGVLGVAR
                     DVTERKKAERHLEEARQAAQRHLLEAEQSRSVLLSLVEDLRAGEQALRDSESRFRAAF
                     NQQFQLMALLAPDGTVLEVNELILHVLGGTRASYLGKPFWSAPAWRQLPEWHSIWPER
                     LARFQHGEEGPLLTEDVFQAADGSFRHADTATTAIRNAHGSLVALLVQANDITGRRQA
                     EDQLRKLSLAVEQSPTSIVITDLAGRIEYVNEAFVRNAGYSRDEALGCDVQQLQDAIP
                     LKDVRTSMWSALREGRIWGGQFINRHKNGDIFYEYAQIAPMRQADGRITHYVWVKENI
                     TAQKRIGEELDRHRHHLEELVHQRTAQLEEARHAAEVANQAKSAFLANMSHEIRTPMN
                     AILGMTHLLRRGPTTP"
BASE COUNT         3405 a         7944 c         6595 g         3163 t
ORIGIN      
        1 agcggtgccc ggtcggtcgg ggccggatgc atcgactccc cgatgtcggt gaaggtgtcc
       61 gcctcgaggg acacgatccg gtcgacgcat gccagcacct gtgccacgca cgcctcgatg
      121 ggcgttcggc tggtatcgag cacgaggtct ggatgctcgg gggcttcgta gggggccgag
      181 atgcccgtga agtgagggat ttccgcggcg cgggcgcgct tgtacaagcc cttgacgtca
      241 cgcgactcgc actcggccag cgggcaggcc acgtacactt ccacaaagcg atccgcgccg
      301 acgatggccc gcacgcgctc ccggtcctcg cgcaagggcg agatgaaggc cgccagggtg
      361 acggtccccg catccgcaaa cagccgcgcc acctcggccg cccggcgcag gttctcgctg
      421 cggtccgccg acgagaagcc cagtccggca cacagcccgt gccgcaggtg gtcgccatcc
      481 agaacaaagg cccgccggcc ctgctcgtgc aggcaggcct ccaccgcgtg ggccagcgtg
      541 gacttgccgg cccctgagag gccggtgaac cacagcaccc gcccgcgatg cccgttcagg
      601 cgttcgcgca tcgcgggtgt cacatcgccc ggatgccaga tcaccgtggg ccgctgcgtg
      661 tccgtcgtca tgccgtggcc cgctggcggt acaggctggc atccacgcag gtcgccatgg
      721 ccaagcagtc cagacgtccg ccgaggaagg cattcacctg ttccgcaacg gccgccgggt
      781 cggcgagggc gtcccggtgg cgcacgaaca gcacatccac ctgggactga cgcgccagcc
      841 acagcttcac cctgcgcagc tgctggcggt agctggcctg cacctgcgca tcgctgagtc
      901 gcgccccttc ccggcccagg ttgtcgagca tgacggtctg ggacttgagc acctcgtcga
      961 gatcgcgctc catgaagatc acgcgacagg gcacgtcgcc gggcaggtag ggcagcaact
     1021 gggccacgat cttgaccgcc tggccccgtc cttcgcggaa ccatgtccgg tcggtccgca
     1081 ggcgggtggc ggcttccagc tcgaaatagc cacgcggatt gtcgaggtcg gcttcacgtt
     1141 tgttgtccgt gaggatcggc agtcctcccg cggcgagcat ctgcatcatc atcgatgtgc
     1201 ccgaacgggg cagaccggcg accacagtga cgaattcggc cgggtcgtcc ggcgtcttgc
     1261 gcacggcgaa cacggcgtcg gcatgcagtc cgggccggcc ggcctcgtcg tccacctcat
     1321 gggcaaggga ttcggcctcg ccgagaccgg gctgaccggt catggacgct gcacgcttgc
     1381 cgagacggat gtgctggatg gcctcgagat gggcccgcgc cttgtcatgc tcgccggttc
     1441 ccagacggta gaagcgggcc agcacccggt gggcacgcac gaagttggga ttgagggcga
     1501 gcgcagtacg catggcatcg gccgccatga agaactgccg caggcgcagc aaccccaggc
     1561 ccaggacgta atgggccagg gggttctggt acagcagccg gatggaatcg agggcggcct
     1621 ccacggcctc ctcggcgcgc gcgcggcgcg gcagcaaggc gcgcgccaca ccgagatggg
     1681 catagggact gagcgcatcg gccgccaggg cccggcggaa ggcggcttcc gcctcgtccc
     1741 agcggtggac cgcaagatgg gcttcgccga tctgcaccag caggcccggg cggcccggat
     1801 ccgcccgctc tgccttgagc aggtgcgcca gcgcttcatc atgacgcccc tcggcgccca
     1861 gcacccagcc catcagatag tccaggtcgg aggtggagaa ccatgccttg aatcgcagct
     1921 cgtccacctg gctgcgctcg tcctcggtga gctggctcag gtcgatggac gccagcgcat
     1981 cggcggcgga cggcggatcg tcctgcgcgc ggacggcggt cgtcgtcgcc acagcctgct
     2041 gacgctggcg caccgtgtcg cagagcgcct gcagggccgc gcgggccgtc tgcgcggctt
     2101 cctgacggtc gcgggtcatg cgctccacca gcgggcgcag ttcggccacc cggttgaggg
     2161 cccggtaaca cagggcgaga tgcacgccga agcgatactg gtccggctcg gcggcataga
     2221 gttcctggag caggggcgcc gcgtcgagca tgcgcccggc gtccatatag ctccgcgcga
     2281 ggttgtaacg cagttcacgc accgtctggg ccacggcctg ttcgcggttg tcgtcgagct
     2341 tttcgatgta accgagggcc accagctgat ccatcacctc cttcgcggcc agggggtcga
     2401 actgctgctc gccggaaagg cgcgcatcgt caccgggtac cgcgtcccag ctgggcacgt
     2461 gggccacctc gggggtccgg tcgaagacat ccagcagcac cttgccatcc atgtcctcgc
     2521 caaccggcag gccgaagagg gtcagcacgg tgggcgtcac gtccagcacg ctggcgccgt
     2581 ggatcaggtg atccctgcgg atgcctggtc cggcgatcag gaacatgccc aggtcgcggt
     2641 gctccaccgc gggaccggcc ggctcgttgg gaatctgcgc gggacggcgg tgatcggaat
     2701 ggaagccgtg atccgagcag atgatcacgg tggtgtccgg cggcgccagc tgaagcaggg
     2761 tgcccagcat caggtcgtgg aactggtagg ccgcctcgac gacatggcgg tacagctcga
     2821 agtcccgctc gggaacaaac ggacgccgcg gcgggtgata gcgcatgaag ccatgggaga
     2881 agtggtcgat ggcgtcgtaa tacacgccca tgaagtccca cggctcgttc gccatggccc
     2941 acgtcgcggc ggcatggacg ctggtgcatt cggcgagggt cttcatcacg gacgccaggc
     3001 ggcggtcctt gtcctggtcg atctccgccg cccgcggcac gaagaagcag acctgctcag
     3061 gcagcagctc attgggattg agccgcacgt cggccagcgc ctgctgcgcc cgagggggat
     3121 ggaccatgcc cggcgccagc ggccagggct ggtccggcgg tccgagggcc gtgtggtaat
     3181 ggttggacac catgaccccg ttgatgggct ccaccggatg actcggccac cagccgatca
     3241 cgtggctctt caggccgctc tggttgagga tgttccagag cgccttggtc ttgcgcgacc
     3301 actggctcac cggctgcacg cccgaaccgt cggggctggg ctcggtgaag cccagaacgc
     3361 cgtgcttgaa ggggcgcttg ccggtggcaa tggtggtcca cagcatcggc gacagcaccg
     3421 gatgcagcgt ggccagattg cccatcacac cccgttcgat cagggacgcg gtgtggggca
     3481 tcaaccccct gtcgacgagg ccattgatga ttttccagtc ggcggcatcc cagccgacca
     3541 ggagcacctt gcgggaagcg ttcatgcgtt gagaattctc ggggaaaggg gttgcggaag
     3601 gacgtccagc cggcgcgggg acgcgaaggc cccgaccatc gatcaggccg ctgccgtcgc
     3661 gtgtgccggg aaatccacga cccacacgtc ctggccgcgg gcgttgcgtc ccacatagcg
     3721 gcagccctgg cgctggaaga gcatccgggc gccgggccgc atgagcttga cgttctcggt
     3781 gacgatcagc atgccggccg cgtcgccctc gcgccgggcc cgcaggcgca ggtgcgcaca
     3841 ggtggcgttg gccaccgcca ggctcaggcg cggtacgcga tccggcttgc gcaggaacat
     3901 gcggaaaacc cagtagatca gtccgctgcg ggtccgcatg cgtccgacgg tcgacacgcc
     3961 tgcgagggcg cctgtgccgt cgtcgcgacg taccagcagg cagacctcgt gggcccgccg
     4021 cacggcttcc ggcagggtcg ggatggcccg ttcgcgcagc cagaactgca cgatctcctg
     4081 gcgctgctcc gagctcgtgt ggaggtggac gttctcgatc cgataggcac cgaagcgcga
     4141 tgaatcaaca tgtctatgca tggcagtcac aggcaccggg gcgccacatc ggcccccggt
     4201 gcagcagaca tcagtgcagc ttgcgacgac gcatcgtggc gatgccggca gccccggccg
     4261 ccagcaggct cagcgcaccg ggctcgggca cgttcagcgt accggcaaag atcgtgcgcc
     4321 cctgatcgtc atacgcccaa tccaccaccg tgatgcggtc ggggaaaccc tgaccgttga
     4381 acagggggct gatgccgccc gcgttttgca ggatgtccga gaacggctga ttcaggccca
     4441 gatcttcgat gttcaggcgg atccagccaa aattgccatt cggcagcgca aagccgaaga
     4501 ggttgtcacc cagcaagagg ttgccgtact gcacggtctt gccgttcacg tagccgacgc
     4561 ccatgcgcac cgcaccggcc gcaccgccga actggttcga accggcaccg atcgccgcgc
     4621 ccggcgcaac cggcgtggca aggcccagcg gggacgagct gccggcaacg aactggacgc
     4681 cggacccctg aacgaccgcc gcgccgatgt acttcgcggc cagccccgca ttgttgttga
     4741 tgttggttcc cagttcagcg gcaatgaccg cgccagccat gaacggcacg ccattgacgg
     4801 agaagttctg gaaattcacg ctgtagccgg catggccgtt cacgtaagaa acgccgaact
     4861 gatgcgccgg atcgatctgg gcgacgagat tctgaacacc gctgtagatc acggtcgcgt
     4921 ccgcattgcc cgccatggcg agcgccgcgc cggcggctgc ggcgtagccg gcaatttccc
     4981 gggcggactt gccggatcgg gcctcgacac ggctgatggc ttcgtagctc tcaagcttgg
     5041 cacgcagcgg cgcgccaaag ttcacgctgg atgaattttg cataaaaacg tccctggttg
     5101 atggtatggg cgggaccgcc aatgcattat tcataccact tcaccaaggc actcattcca
     5161 atgggatttg ccggctgcgc cagacgggtt cggcaaaaaa cgtaaaattt catgacagat
     5221 tggcggcaat tcaggtcgcc cagagcgcaa aagtgcggca tccggcctgg ccgcgcgacg
     5281 tcccgaccgg caccggagca gcccccatcc cgccatccca ttgaaataat gaggtttgca
     5341 tcacaaccga tctcatcccg gaggcgggcc gacctgccaa aaagtgtaag attttccgac
     5401 acccgcccgc tggtcgatgc ccggcgatgg gcgtgcgcgc cgtccgcccc cccttccgtg
     5461 agcgctcgcc accgcatgac gtcgcccgga tccgccacac gacgcccgcc gccagnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagcgc tcgccaccgc atgacgtcgc
     5641 ccggatccgc cacacgacgc ccgccgccag gcgcgtgggc gagagaccga cggcacagca
     5701 tcctttccgg agccccagcc cttcccgggc cgcgatgacc ggacgcgaac cggtcaattc
     5761 ccctcgcccc cggtagatcg tcgtgcggca cgcgcctgaa aggacttagc agcgcgccgg
     5821 gacaggtctg ccgcgcccgg tcggacttga gggcagtcgt ggccggagcg gtcagtatcg
     5881 cgggcgttag cgccgcgccc tcatgccggc cgcaccgacg gcggacgcat ggccggcgaa
     5941 tgcgaggcgt gaagcattcg ggcggccctg gcagccggca cgcgcgtcat aggcgtcaag
     6001 gcgccggcgc aggctcgagg acaccccacg gacattccgg gggcgcctga cgcctcctga
     6061 cctcgtaaac ggtgcggagc ttgtgccatc cacatgctcc gatcagaacc cctgttgcac
     6121 ggaaacattt ctcgcgcagg cgcaacgctg gaggggtgtc tgtaaaactc aagggcatgg
     6181 aggtcccggc atgcggcaag cgccgcgcgg cctggtgcat gcatgcgggc gccgcgccgg
     6241 atctggtgcc tgccatctga tccggcgtgc aggccgcagt cctactccgg cgaccgaatc
     6301 cgcaccgtct gcgcccgccg ctcccgctcc gcctgacgcg cggtcgccgc catgctctcc
     6361 tccacgaagg caatgtgccg ctcgacggca gcctgggcgg cgcccgcatt gcgggtgcgc
     6421 agtccttccc agatcgccgc gtgctggcgg cgcaactcct gccaatcgtc ctcaagccgg
     6481 cgcaggtggc gcaggttgcg gtcgatattg tcgtgcgtga cacggaacag gctggccgtc
     6541 agatgaccga tcatcacgtt gtgggaggcc tcggcaatcg cctgatggaa ggccagatcg
     6601 gcgcgcacct gctcggcgaa gacctccgcc gacggccctt gggcgaacag gccttcgacc
     6661 cgcgcatacg cgtcgccgat ccgttgcaga tccccgtcgg tggcgcgccg cgcggccagg
     6721 ccggcggact ccgtctcgag catgcggcgg aattccagca ggtcggactg gatgtccgga
     6781 tgctggcgca gcatctcgct ccagggatcg gtgaagccgg cttcaaggcg gtccgtcacg
     6841 aaggtaccgc cgccctgacg gctgtgcagc agccctttcg acacgagttt ctggatcgcc
     6901 tcccgcaggg acggtcgcga cacgcccagc tcggccgcca gctcccgctc cgcctgcaga
     6961 cggtcgccag gcttgagcga gccttccaga atgcgccgct cgagttcgtg ggcgatggtg
     7021 tcggaaaggc gctggaccgc gatcttgctg ctgctcatgt catgctcaaa aaattggtct
     7081 gaccagaata ctacacagcc atcccccgtt cctcaaggca gggccccgcc gcgggtctcg
     7141 gtgtttaccc ttatatcact caaaaatcag gggtttgcac gctttcgggg attgacttcc
     7201 gggagcgcgc cgtttagatt tccactccac cgaataactg gtcttaccag tttaccagtg
     7261 aatacacccc ggggccttga gccccaccgc cgcgggaacg gcccacaatg ccggcttcgc
     7321 agcaggacaa ggaggagacc atgagcgaca cccttcccgg cggcgggccg acgccccgcc
     7381 gctacccgtc cagaccggcc gcggtttacc tctatgcgac ctgcctggtt gacctcttcg
     7441 tgccggaagc cggcctggac gccgtcaccc tgttggaacg ggaaggcctc cgcgtgatct
     7501 ttccggagaa ccagacctgc tgcggccaac ccgcctacac cagcgggttc ccggacgagg
     7561 cccgtcaggt ggtggcctcc caactggacc tgttccccga ggactacccc atcgtcgtgc
     7621 cgtccggctc ctgcgccggc atgatgcgct ggcactggcg tggcgtggtc ggcgatgacc
     7681 cggccctcgc cgcccgcgcc gcggcgctgg ccgagcgcac cttcgagctg gctgaattcc
     7741 tggtgcacgt gctcgatttc cgccgccccg acaccggcgc gcccaccacc gtcaccctgc
     7801 acacctcctg ctccgcgcgt cgggagatgg gcaccctgct ggtcggacgg gaactcctcg
     7861 cccgcctggg gcaggtgacg cttgcggtgc acgatcacga atccgaatgc tgcggcttcg
     7921 gcggcacgtt ctccctgaag catccggatg tctcgaccgc catggtcggc gacaaggtca
     7981 acgccctgcg cgccaccggc gccgcccaga tggtcacggc cgactgcggc tgcatgctca
     8041 acatcagccg ccgcgcggcg aaagaagacc gggatgccgg ccgcgtcggc gcatccctgc
     8101 cggccgaaca cctggccagc tttctgctgc gccgcaccgg aggcaagccc gcatgagcgc
     8161 ccgcaacagc atcctcgccc gcctgcgcgc cacccggccg gccgccgccg atcccctgcc
     8221 cgacctggac gcccactttg ccccgcgtgc acgccacgaa accgtcgccc agcgggcatc
     8281 ccggttccgc tccggcatcg aggccttcca cgccgaggtg catgtctgcg ccgacgccga
     8341 ctggccggag ctgctcgccc gcctgtgcgc ggccaagggc atcggcacgc tgctctacgg
     8401 ccgcaacacg cccgccggtg aacgcatcga cgcgacaccc tttggcgcca cgcagatacg
     8461 tccctggcag ggccgcttcg aagagctcaa ggccgacctc ttccaccgca tcgacgccgg
     8521 cttcaccacc gcccgcggcg ccatcgccga gaccggcagc ctgatcctgt ggccctcgac
     8581 ggccgaaccc cgcaccctgt ccctggtccc gccggtgcac ttcgtgctgc tcgatgcccg
     8641 cagcatccac gccaccttct tcgatgccct gcgcgccgag cagtggtcag accgcctgcc
     8701 caccaacgcc ctgctgatct ccggcccgtc caagaccgcc gacatccagc agaccctcgc
     8761 ctacggcgcc cacggcccga aggagctggt ggtgatcctc acccacgccg aaggagcctc
     8821 agcatgacag cgccccgtcc tgccggcgac gcccgcccga tcgccatcgt ctcggccgaa
     8881 gccctgcgcc agcgcatcca cggcgcggtg aacgatcccc acctgcgcag cagcttccgc
     8941 ggcgccatgg atttcctcat ggccaagcgc gccgcccagt tcccggacgc cgacgagctg
     9001 gcctccctgc gcagcctgtc cgagtccatc cgccagtaca gcctgtccag gctgcccgac
     9061 ctgctggaac agctcgaagc gcgcctcacc gccaatggcg tgcacgtcca ttgggccgaa
     9121 acgcccgacg aggccaacgc cattctcctc gacatcgccc ggcgccatgc cgcgcagctg
     9181 atcgtcaagg gcaagtccat ggtgagcgag gagatcgagt tcaaccacgc catgcacgcc
     9241 gccggcatcg acgcgctgga atcggacatg ggcgaataca tcgtccagct ggccggcgag
     9301 aagccctccc acatcatcat gccggcgatc caccagacca agcagcagat cgcccgcctg
     9361 ttcgccgaca agatccccgg cgtcgattac accgacaacg tggacgccct catccagatc
     9421 ggccgccggg tgctgcggga gaagttcgag actgccgaca tcggcctctc cggcgtcaat
     9481 ttcgcggtgg ccgagaccgg caccctgtgc ctcgtggaga acgaaggcaa cgggcgcatg
     9541 tgcaccaccg tgccgcgggt gcatgtggcc atcaccggca tcgagaaggt cgtcgagaag
     9601 ctggagcacg tggcgccgct gttttcgatc ctgacccgct ccgccaccgg ccagtccgtc
     9661 tccacctatt tcaacctgat ctccggcccg cgcaagcccg acgaacggga cggccccgaa
     9721 gaggtgcacc tggtcctcct cgacaacggc cgctcccagg cctacgccga cgcccagctg
     9781 cgcaagaccc tccagtgcat ccgctgcggc gcctgcatga accactgccc ggtctacacc
     9841 cggatcggcg gtcacgccta tggcaccacc taccccggtc ccatcggcaa gatcgtctcg
     9901 ccccacatga tggggctcca acagacccat ctgatgccca gcctctccag cctgtgcggc
     9961 gcctgcggcg aagtctgccc ggtgaagatc cccatccccg agctgctcat tcgcctgcgg
    10021 ggcgaggccc agtccgcacc gcaggcaaac cccgtgatgg cgggccaggg tgccggccac
    10081 gaccgcctcg tcagcttcgc ctggcgcgcc tgggcacggg tgtacgccag cccgctgggc
    10141 taccggacct tcagctggct ggcgagccgc ttccgcgccc tgacgccgac ccgccaggcg
    10201 ggctggaccg ctgcccggct gcccctgcaa cccgctccac gaaggctccg ggacatgctg
    10261 cgcgaccgca gatagcggac agcccgtcgg ggccgcctgc ggccccgccc cggcgcgtcc
    10321 gacgaccgcc cccgccaccc taccgaatcc acctcatccc tcacgggagg aaccaccgtg
    10381 tccgcgctca tcgatgccct gcgccagcga ctgcccgcca gccgcgtgat caccgacgaa
    10441 ctgcgccgcc tggcctatgg caccgacggc agcttctacc gcctgattcc cgaagtcgtc
    10501 accgtcgtgc acgacgaagc cgaagtccgc gacgtgctcg acctcgcccg ccagcacggc
    10561 cgcagcgtca ccttccgcgc cgccggcacc agcctgtgcg gccaggccgt caccgatggc
    10621 gtgctggtgc tgctgggcga aggcttcgcc gcctgccgca tcgccgacga cggcgcccac
    10681 gtggccgtcg gacccgccat cgtcggcgcc gaggtcaacc gccgcctcgc ccccttcggc
    10741 cgcaagatcg gccccgaccc agcctccatc aatgccgcca agatcggcgg gattgccgcc
    10801 aacaacagtt ccggcatgtg ctgcggcacc gcccagaaca gctaccgcac cctcgccagc
    10861 ctgcgcgtga tgttcgccga cggcagcgtg ctggacaccg gcgacagcgc cagccgggcc
    10921 gccttccaga agagccacgg cgccctgctg caaggcctcg ccgagctggc caccgcggta
    10981 cagggcgacg ccgccctcgc cacgcgcatc cgcgccaagt acaagatcaa gaacaccacc
    11041 ggctacagcc tcaacgccct ggtggatttc gccgatccga tcgacatcct cgcccacctc
    11101 atgatcggct cggaaggcac cctcggcttc ctctccgaag tccgctacca caccgtgccc
    11161 gagtacgccc acaaggccag cgccctggtg ttcttcccgg acatggagag cgcctgccgg
    11221 gcggtggtcg gtctcaggca gcagccggtg gacgcggtgg agctcctcga ccgggcctcc
    11281 ctgcgggcgg tcgccgacaa acccggcatg ccgcccctgc tccagaccct cgccgacggc
    11341 gccaccgccc tcctcatcga aacccgcgcc cccgatgccg tcaccctcga cgcccgcatc
    11401 gccgccgtac tggccgaact ggcccgccac ccgctcaccg agcctgccgt cttcaccacc
    11461 gcgccggccg agatcgagcg cctgtggaac gtgcgcaagg gcaccttccc ggcggtcggc
    11521 gccgtccgca agccgggcac caccgtcatc atcgaagacg tggccgtcgc cgtggaggat
    11581 ctggcggcct gctgcctcga cctgcagcgc ctgttcgccg cccatggcta ccacgaagcc
    11641 atcatcttcg gccacgccct cgaggggaac gtccacttcg tgttcaccca ggacttcggc
    11701 atcgaatccg aagtggcccg ctacgccgcc ttcatggacg agctgtgcac cgtgctcgtg
    11761 ctgaagtacg acgcctccct caaggccgag cacggcaccg gacgcaacat ggcgcccttc
    11821 gtcgagctgg aatggggcag cgaagcctac gccctgatga aggccatcaa gcagctcttc
    11881 gacccgcaag gcctgctcaa ccccggcgtg atcatcaacg acgacgccga ggcccacctc
    11941 aagcacctca agcccatgcc cgccgccggc cagctctacg cgccggtgga ccgctgcatc
    12001 gagtgcggct tctgcgagcc ccagtgcccg tcccacggcc tgaccctgtc gccgcgtcag
    12061 cgcatcgtcg cctggcgcga gctgtcccgg cgcgcagccg ccggcgaggc gccggacacc
    12121 ctcgacgcgg attacaccta catgggcctg gacacctgcg ccgcctgcgg cctgtgttcc
    12181 accgcctgcc cggtgggcat cgagaccggc gccctgaccc gcgcggtccg tggcgagcgg
    12241 ctctccggcg tggcccggcg cctggggcgc ctggcggcgg aacacttcgg cgccacccag
    12301 gccctcgccc gcaccgccct caaggccggt catctggcgg aatccgtgat cggcacccgc
    12361 gccctcgcgc gtctcagcgg cggcgcctgg aagcgcggca tgccccatcc ccagcccgcc
    12421 ccccgcgcgt cccgctcggc gggcgacccg gtggtttatt tccccacctg tgcaggccgc
    12481 atcttcggcg ccgacgcgcc ggagcagact ctctccgcca ccgtcatccg agtaatggag
    12541 cgggctggct acgcccccat cgtgccggac ggcgtcgatg ccctgtgctg tggccagtcc
    12601 ttcgccagcc gcggcctcgc cgacgaggcc gaccgcaagt ctgccgagct ggaagccgtg
    12661 ctgcgtgccg ccagccgcga cggcgcgatc cccatcgtcc tcgacgccag cgcctgctcc
    12721 ctgcgcatga agaccttcct cgccgggcgg ctgccggtcc tggatctggt ggagttcgcc
    12781 catgacgcgc tgctgccgcg cctgatgctg caacgcaagg ccgacccggt gctgatccac
    12841 ctcaactgct cggcccggcg catgggcttc gagacccaac tcaaggccct cgccgccgcc
    12901 tgcgcggagc agcccgtgct gccgcccgag gtgaagtgct gcggcttcgg cggcgaccgg
    12961 ggcttcgtcg tgccggaact caacgcccat gccctgcgca agctggaggt gccggccggc
    13021 tgctgcggcg gctactccag caacgccacc tgcgagatcg gcctcaccga caccaccggc
    13081 ctgccctacc gctccatcgt ccatctgctg gacgaatgca gcgccgaggc ggcccgtatc
    13141 gccacctgct gaaccccgcc cgcccccgac ccgactttcg acccgcattc acccaaggag
    13201 gaaacacacc atgcaaacct ggatgcagat ctacgatcca gccggcaacc tctggttgtc
    13261 ggccctggtc gccgcactgc cgatcatctt cttcttcatc gccctcgcgg tgctgcgcct
    13321 gaagggccac atcgccggca cgatcaccgt cggcatcgcc ctcgcggtgg ccatcttctt
    13381 ctacaacatg cctgtggaga tggctttcgc ctccgccggc tacggcttcc tgtacggcct
    13441 gtggcccatt gcgtggatca tcatcgcggc ggtgttcctc tacaagatca ccgtcaagac
    13501 cggccagttc gacatcatcc gcgcctcggt catctccatc accgatgacc agcgcctgca
    13561 gatgctgctg gtgggcttct ccttcggcgc cttcctggaa ggcgcggcgg gcttcggtgc
    13621 gccggtggcg atcaccgctg cgctgctcgt cggcctgggc ttcaaccccc tctacgccgc
    13681 cggtctgtgc ctgatcgcca acaccgcgcc ggtggccttc ggcgccatgg gcatccccat
    13741 catcgtgggt ggccaggtga ccggcatcga ccccttcaag atcggccaga tggccggccg
    13801 ccagctgccg ctgctgtcgg tcatcgtgcc tttctggctg gtggcgatga tggacggctg
    13861 gcgcggcatc aaggagacct ggcccgccat cctcgtggcc ggcggcacct tcgccgtcac
    13921 ccagttcttc accgccaact acatcggacc ggaactgccg gacatcacct ccgccctggt
    13981 gagcctggtg agcctgaccg tcttcctgaa gaactggaag cctaagaaca tcttccgctt
    14041 cgacacccag caccacgccg aactgggcga ggaccagcac tacagcctcg gccagatcgc
    14101 caaggcctgg tcgcccttcg tgatcctcac cgtgatggtg acgatctgga gcctcaagcc
    14161 cttcaaggcc ctcttcgcca aggacggccc cctgtacagc ctggtgctcc agtggcccgt
    14221 gcccatgctc gacaagctgg tcatcaagat ggaacccatc gtcgccaagg ccacgcccta
    14281 tgcggcggtg tacaagctgg acctgctctc cgccaccggc accgccatct tcctgtcggc
    14341 gctgatcagc atgctcgtgc tgggcctgcg cggcggcgag gcggtcaaga ccttcaagga
    14401 gacggtgctg gagctcaagc gcccgatcta ctccatcggc atggtgctgg ccttcgcctt
    14461 cgtggccaac tactccggcc tgtccgccac cctggccctg ctgctggccg gcaccggcaa
    14521 gctgttcccg ttcttctcgc cgttcctggg ctggctcggc gtgttcctga ccgggtcgga
    14581 cacctcgtcc aacgccctgt tctgctccct gcaggccacc accgcccacc aggtcggcgt
    14641 gtccgacacc ctgctggtgg cggccaacac caccggcggc gtcaccggca agatgatctc
    14701 gccccagtcc atcgccgtgg cctgcgccgc cgtgggtctg gtgggcaagg aatcggacct
    14761 gttccgcttc acggtcaagc acagcctgct gttcgccacg atggtgggca tcatcaccac
    14821 cgcccaggcc tatctgttca ccggaatgat tccgtaagca ccgggtttca ccaggacggg
    14881 gctctgcccc acgttgcctt gacggcgacc ggagcgatcc ggtcgccgtt ttttttgatg
    14941 cggacacacg gcagcacccc gaaccaaaga ccacactgca aaaatggata tcccttgacc
    15001 tccgtcaaga tccgccaggc accgaggcgt aggatcggcc cggctgtaat ctttccgtca
    15061 cattcaggaa gaggcggagt cacccatgca caagaagatc gttgtttccg ccatcgccgc
    15121 cattgccgcc ctgtccgccg gggtcgctca tgccgaaagc ccgtggatgg tccgcgcccg
    15181 cgcggtcaat ctcgactggg acaacaagaa cgccgacaac ctgcagaacg ccggcgtggg
    15241 cgacgtgagc gccaagaagc gctggatccc ggaagtggac atcagctact tcttcacccc
    15301 gaacatcgcc gccgaactgg tcctcaccta cccgcaggac gtggacatcc agacctccgc
    15361 cctgggcaag atcggcaagg tccaggccct gccgccgtcc ctgctggtgc agtaccactt
    15421 caccgaactg ggcgccttca agccctacgt cggcctcggc gtgaactaca ccatcttcac
    15481 caagaagagc tttgacaccg ccctcggcac cgtgtccgtg gatcgcgcca gcttcggctt
    15541 cgccgcccag gtgggcgccg actacaagct ggacaagaac tggtccctca acgtggacgc
    15601 caagtacatc cagatggaca ccgacgtgaa gctgggcggc agcaagattg gcaagctcga
    15661 cctgaacccc gtcacctggg gcgtcggcgt cggctaccgc ttctgatcgc atcacgcctc
    15721 ccggcacatg gacggggcac cctgcggggt gccccgtttt catgggcctg ccgcaccctc
    15781 tgccgcaggc ctgtgctgac gcggcatcct gcccgccagc gccccgggcc cgcggacagc
    15841 atccggaggc cctgctcccc acggtgagcg cgcctcgcca agggtgccag gcaccggcac
    15901 ggcgaacatc cccgccggac gacgccccga ccgccgcgac ggtctggcgc tgcggctaca
    15961 atcggcgcca ccctcctctg cgccgcccgc catgccgcct ctcccgcccg acgaatgccc
    16021 gcctgacgag ccgcccgcgc cgcaggccac caccacgctg accgttcggc gtctcgacgg
    16081 cccgcagctg cgggccacag aggacaccgt cgccgaagag gtgccggtgg cgctggtcta
    16141 caacggcatc tcccacgccg tgctcctcgc cacgccggcc gatctggagg atctggccct
    16201 gggcttcagc ctctccgagg gcatcctcgc cacgccgacg gagcttttcg acagcgagac
    16261 cgtcgccacg ccgcacgggc tcgaactgcg cctgcatgtc gccgccggat gttttgccga
    16321 cctcaaggcc cgtcgccgca gtctggcggg gcgcacgggc tgcgggctgt gcggggtcga
    16381 gagcctggcc gccctcgccc gcgcaccggg cccggtgacg cgcggcgcgc cgatacccgc
    16441 ctccgccatc gccgccgccc tggccgcgct gccggcccat cagcccctgc gcgccaccac
    16501 cggcgccgtg catgccgccg cctggatcga ccgccggggc acgctcctcg ccctgcggga
    16561 agacgtgggc cgacacaacg ccctcgacaa gctgctgggt catctggccc gcagcgctac
    16621 cgacccgcac gccggctggg tgctcgtctc cagtcgcgcc agctacgaga tggtgcagaa
    16681 ggtggccaca gtgggcatcg ggagcctcgt agcggtctcc gcgcccaccg ccctagcgat
    16741 ccgcctggcc gacgccgcgg gcgtgaccct cgccggcttt gcccgcgacc gtcgcctggt
    16801 gatctacacc cacccggacg gattgctcgc cgacgcctga accggtctag gtccagcgca
    16861 ggcgctggct gcgctccaga cgccgcacga agtccggaaa atccagtccc agacgggcac
    16921 gtagcgccct ggcctgattg gccagctcgc gccagcgcgg cgcgaagccc tcggcccgcg
    16981 cggcgagcac gatgtcgttg cccccgtcgg gcaccgtcag tgaccacacc cgctcgccga
    17041 aggccgccgc gatcatgtcc cggtgggcga cccgctccgc cttgtcgccc gccaggttgg
    17101 ccaccagcca gccacgcccg gcgagggccg cgcagacctg cccgtagaac gtctcgttgc
    17161 tcaccgagcc ggacagcccg tcccggtcga aggcgtccag caggatcacg tccggccgct
    17221 cggaacagtc cgccacccag gccgccccgt cgccctggat cacctgcagg cgcccatcgt
    17281 ccggcggcac ttcgaattcg tcccggaatg cgatcacatg ggggtcgatc tccaccaccg
    17341 tgagccgcgc cgaccccaga tggcgatggc agtacttgac cagcgagccg ccccccagcc
    17401 ccagcatcag caaatgccgc ggacgtggca ggaagagcag gaaactcatc atcgcccgcg
    17461 tgtagtccag ctcaagcgca aagggatcct tcaggctcat ggtgctctgg atcagggcca
    17521 gcgagaaatg caggctgcgg gtcgtgccgt cgtcgaggac gaagggcttg tcgtagtccc
    17581 ccgtctcgac ccgtcgcaat acggcggacc ggtcgcactc cggtgactcc agcaggccga
    17641 tccggtcgct ggggcagaag ggactgggca gggtcaggac ggacgaggac acggagacag
    17701 gaggaccggc aggcatgaag gcgccattgt acggtgacgg atcgtcaccc cgcgcccaca
    17761 gacgagacgc cgccgcgcct cgggggtaca ctccgcactc tggccctttt cccgctcccg
    17821 tccgccatgc agcgtcaccg ctacctgcgc ctcagcgaca tcgccaccac gtcggttcgc
    17881 accgttcttc cggacagccc tctacgcgac gccatcgccc tgatggccca gcagcacgtc
    17941 tcctgcgtgg tcgtcaccga aggtctccgc ccggtcggca tcctcacgga acgggacatc
    18001 gtgcgcgtca tgagcaacgg cctccggccc gccacggcga tcggcgacgt catgagccag
    18061 ccgctgctca cggcccgcat ggacctggac tttccggccg cgcagaatct cctcgcgcgc
    18121 cagggcatcc gtcacctcgt ggtcaccgac gacgcgggcc agctgtgggg aatcgtcacc
    18181 gagagcgact tccgggccca cctgaaccgg gacctcttct cgcgcatccg cagcatcgaa
    18241 tcggtgatgg acccggagtt caccaccctg ccacccgacg ccccgctgca acgcgccctc
    18301 gactgcatga gcacgggccg ctgcgaccac gtggtcgtca cccgccaggg cagccccgtc
    18361 ggcatcctca ccgaacgcga cgtgccgcgc ctcatgctgc gcggcgtgaa cccgtccacc
    18421 accgcgctcg atcaggtcat gtcctcgccg gtgcagtcca tccgcaccca cgacaccctg
    18481 gccaccgcca ccgaccgcat ggatgccgcg ggcatccggc acatggtggc cctcgacgaa
    18541 caggatcgcc tggccggcgt ggtcagccag caccgcatgc tggaacgcct cggcgcgacc
    18601 ctcctggacg agatgcgttc ccgcctgcag gatcgcctgg acgtgatcct cgacgccacc
    18661 ggcgtcggca cgtgggaata cgactacgcc gccggacgcc tcacccgcaa cgccgccctg
    18721 tggaagctgc tgggcgtgga cgcgccctcc ggccgtgacg aaaccctgga tgcggccttt
    18781 gcccgcgttc accccgacga cctcccacgc ctgcgcacca tcatcgaggc cgcccgcgag
    18841 cagcgcaacg gcaatgacga gcccttctcg tacgagtacc gggtgctgcg gcccgacggc
    18901 agctggctgt ggctgcgcac gatcggccgc aatgtggacc gggacgacga cggactggcg
    18961 ctcggcgcgg cgggcatcac catcgacatc tcgggcgaaa aggccgccac cagccgcgcc
    19021 accgaatccg agcaccgctt ccggcggctc atggagcgct tgccgatccc cgtcatgcac
    19081 atcgcccggg gcggacgctg cctgttcctc aacgaccact tccagcagat cttcggctac
    19141 accatcgacg acctccccca cagcgacgcc tggcgccagc gggccttccc cgaccgggac
    19201 ctgcgtgccc gcgccgacgc cgcctggcgc gaggccttcc tgaccgcccg acgccacggc
    19261 caggccatcg ccccgcagga atttcgcgtc gtctgcaagg atggctcggt gcgcatcgtg
    19321 gacgcctcgg gcatcgtgct cggcgacgac ttcctcgcca ccttcgtgga cgtcaccgaa
    19381 cgcaagtccg tgcaggagca gctgtccctg cgcctcgccg aactcaaccg ctggtacgac
    19441 gccatgctcg gccgcgaagg ccgcgtgctc gagctcaagc gcgaagtgaa cgccctgctg
    19501 cgcgccaccg gtcagccgat ccgctacccc agcgccgatc agccgtccga ggggcgcccg
    19561 gcgtgacgcg cctgcgcttg ccgcggcggc cgctcgccgt tctgctggct gcctggcccc
    19621 tcgcagcccc cgcgatggac gccgcatcgt ccgcacccgc actgtcggcc ctggcctgga
    19681 tcgccgtcgg cgccctgggc gcagtcgccg cgaccgtcgc gctgcggcgc cggcacggca
    19741 acgctgatgc agacgcccgg acctccacgg acgccgaatc cctgcgcctg cagacgctcc
    19801 tgcacaccat tcccgatctg gtctggctga aggatccgga tggcatctac ctggcctgca
    19861 accgggcgtt ccagacctac ttcaacgctc ccgaagccga catccttggt cgcagcgacg
    19921 ccgatttcgt cggcgccgaa catgccgccc gcgtccgcga cgacgacgcc gcggccgtcg
    19981 ccgccggcac cccgaccgcc accgagcgct ggagcattca ccgggccacc ggcgcgcagg
    20041 tcgcccacga gatcacccgc acgcccatgt tcgccgccga cggcagcctc atcggcgtcc
    20101 tcggcgtggc ccgagacgtc accgaacgca aaaaggccga gcgccacctg gaggaagccc
    20161 gccaggccgc ccagcgccat cttctcgaag ccgagcagtc ccgcagcgtc ctcctcagcc
    20221 tggtggaaga cctgcgcgcc ggtgagcagg ccctgcgcga cagcgaaagc cgcttccgcg
    20281 ccgccttcaa ccagcagttc cagctcatgg ccctgctcgc cccggacggc acggtgctgg
    20341 aggtcaacga acttatcctg cacgtgctcg gcggcacccg cgccagctac ctgggcaagc
    20401 ccttctggag cgcgccggcc tggcggcagc tacccgagtg gcacagcatc tggcccgaac
    20461 gcctggcccg cttccagcat ggcgaggaag gtccgctcct caccgaggac gtcttccagg
    20521 cggccgatgg cagcttccgt cacgcggata ccgccaccac cgcgatccgc aacgcccacg
    20581 gcagcctcgt tgccctcctc gtccaggcca acgacatcac cgggcggcgg caggccgagg
    20641 accaattgcg caagctgtcc ctcgcggtcg aacagagtcc caccagcatc gtcatcaccg
    20701 acctggccgg gcgcatcgaa tacgtcaacg aggccttcgt ccgcaacgcc ggctacagcc
    20761 gcgacgaggc cctcggctgc gacgtccagc agctccagga cgccattccc ctcaaggatg
    20821 tgcgcaccag catgtggagc gccctgcggg aaggccgcat ctggggcggc cagttcatca
    20881 accgccacaa gaacggcgac atcttctacg aatacgccca gatcgccccc atgcgtcagg
    20941 cggacggacg catcacccac tacgtgtggg tcaaggagaa catcaccgcc cagaagcgca
    21001 tcggcgagga gctcgaccgc caccgccacc atctggaaga gctcgtccac cagcgcaccg
    21061 cccagctcga agaggcccgc cacgcggccg aagtggccaa ccaggccaag agcgccttcc
    21121 tggccaacat gagccacgag atccgcacgc cgatgaacgc catcctgggc atgacccacc
    21181 tgctgcggcg cggccccacc acgccgg
//
LOCUS       DAOQMM010000063        20876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_905_length_20876_cov_52.898084, whole genome shotgun sequence.
ACCESSION   DAOQMM010000063 DAOQMM010000000
VERSION     DAOQMM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20876
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_20876_cov_52.898084"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLH85_11010"
     CDS             <1..750
                     /locus_tag="PLH85_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB65141.1"
                     /translation="ADVVNKIRRSPNAAEFIRLFGSDILDRPDDAFARLLLALQQYQT
                     EDPDFAPFTSKFDFWQAGKAQLTAQELRGWALFNNPVKGNCAACHISTGQNGVPALFT
                     DFTYDTLGAPRNMDIAANADPAYYDLGLCGPSRSDLAARTDLCGAFRVPSLRNVAATA
                     PYFHNGSLKTLTDVVRFYVRRDTNPEEWYPTDSAGVVQKFNDLPEAYRRNVNTTEVPY
                     NRKPGDSPALDASEIADLVAFLNTLTDGYTP"
     gene            789..2126
                     /locus_tag="PLH85_11015"
     CDS             789..2126
                     /locus_tag="PLH85_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNB65142.1"
                     /translation="MKTTPLSLALAACFLSSGAQAATDAELLARIDRLAAELEAVKAE
                     LRSVKAQATPPAPITTTAATTPAATVPVPAAPGAYAQTASASGANPTSLFGYGEVNYN
                     RPFRNSSDTRMDVRRAVIGVTHQASERTRIVGEFEFEHAVASATDSGEVEVEQLYVEH
                     RLNDNVGLKGGLFLIPLGTLNLSHEPLSYYGVERNFVETAIIPTTWREVGLSTFGVTD
                     EGLRWDVGLTSGFDLNKWDKTSDEGRTEGPLAAIHQEGSQARGHDLSVYAALDWRGTP
                     GLAVGGGVFSGKASHKVNDPSTANAGVYAPGARVTLWDTHARWTPGKWDLAALYARGS
                     ISDTDQLNVQLAGGTPVPKSFFGWYTQAAYRLWENGEYKLSPFVRHERFNTGATYAAM
                     PAGLEIDPLRTEKVLTAGANFQVGGNVVLKADVQRFVVDRSRDRLNLGLGYQF"
     gene            2211..2783
                     /locus_tag="PLH85_11020"
     CDS             2211..2783
                     /locus_tag="PLH85_11020"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNB65143.1"
                     /translation="MKHSRHEWPVVPASLLIAAGALATFQAPHAIAAQYMSAEQAQKT
                     AFPDADRFDVRPLRLTPEQLRTLDGVAPVRQRGELRQILAYAGPRLLGTLYVDDVIGK
                     VEWITYALAINPDGSVRSLDILEYRETHGFEVRTASWRKQFVGHRSDTPLHFGDDIKN
                     ISGATLSCAHLTAGVQRLLKLHAQLASAER"
     gene            2837..3703
                     /locus_tag="PLH85_11025"
     CDS             2837..3703
                     /locus_tag="PLH85_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNB65144.1"
                     /translation="MEATAAEQRVADAAVAAAFDAVADVHRLMSFHAQDSDLANLHAD
                     AWRQPVTVSPHTWQVILHAQTVAAASDGVFDPTVAPALVASGALPAPTGPRPDPTATW
                     ADVELLDGQQIAFRRPLWLDLGGIAKGYAVDRAVHILCEYGADSGLVNAGGDLRVFGR
                     HRTRVPVAVRHPAQPSQTLPLGSLAQGAVAGSGDYFAAMPAEQASPIVAPGRGLRPAG
                     RTAVVVVADECWCADALTKVVSLMESASSPVLRMFNATAALLDAHGTLRAMPGFMERL
                     GHATPEEQSSHA"
     gene            3696..4211
                     /locus_tag="PLH85_11030"
     CDS             3696..4211
                     /locus_tag="PLH85_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65145.1"
                     /translation="MHEFPHRPRHKAVRPGRRQRHSLYAVAAVLMVSGLVWLVAHYAL
                     PLPDDAARHPAEPWAMRIHGAATLLGMAVLGALWSHHVLPAWRAGEKRATGLPLLGIW
                     LALAVSGYGLYYLAEESWRDAADTLHVSAGLAMPLALASHVWMQGRRQKKGERPSSGP
                     HPLSPQSKETG"
     gene            complement(4281..6428)
                     /locus_tag="PLH85_11035"
     CDS             complement(4281..6428)
                     /locus_tag="PLH85_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB65146.1"
                     /translation="MIVLFVSALLGLAVAGCDGVWNDPYPVAERGQNILYSAFTQRPK
                     HLDPVQSYSEDEATFLYQIYEPPLQYHYLKRPYQLEPATAATLPVLRQYDRAGHELPP
                     DADPARVARSTYEIRIRPGVRYQPHPAFAVDAAGQARYVALSRSDIAQRRRPSDFPES
                     GTRELTADDFVYQIKRLAHPRLHSPVLELMGDYIVGLKDLNARLVAEERGRADKGAWI
                     DLRRYDLPGVEVVDRYTYRVHLQGTYPQFPYWLAMPFFAPVPFEADRFFAQPGMADNN
                     LTLDSWPVGTGPYMLVENNPNARMVLARNPNFRGERYPCEGEPDDRAAGFLEDCGKVI
                     PFVDTVVFTREKESIPYWNKFLQGYYDASGVSSDNFDQAVQMGAQGDVALSDEMARKG
                     IRLLTSVAPTVMYMGFNMLDPLVGGDSERARKLRLAISIAIDQEEFISVFQNGRGVPA
                     MHPIPPGITGYRVDQAGINPYVYDWVDGKARRKSVDDARRLLAEAGYPNGRDAKTGEP
                     LVVNLDTTGSGMGDKSTVDWLTKQLNKLDVQLVVRATDYNRFQDKIRKGNAQLFYWGW
                     NADYPDPENFLFLLYGPQSKVKNQGENAANYANPEYDRLFERMKAMPDSPERLALIAR
                     MLAILQHDAPWVWGFYEKAYTLQHGWVSNRKPGKIVRSTLKYQRIDVTTRSAMRAEWN
                     RPVVWPLAVLLILLVVLALPAVAHYRRREQGNE"
     gene            6537..7331
                     /gene="fabI"
                     /locus_tag="PLH85_11040"
     CDS             6537..7331
                     /gene="fabI"
                     /locus_tag="PLH85_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNB65147.1"
                     /translation="MGFLAGKRILITGLLSNRSISYGIAKAMRREGAELAFTYQNERF
                     KERVAKLAAEFDSTLIFPCDVQNDAEIASLFEQLGTQWDGLDGLVHSIAFAPGEALEG
                     DFLDGLTREAYRVAHEVSAYSFPALAKAARPMMKGRKGSLLTLSYLGAVRTMPNYNIM
                     GLAKASLEASVRYMAVSMGPEGTRVNGISAGPIKTLAASGIGSFGKLLAFNEHNAPLR
                     RNVTIEEVGNAAAFMCSDLASGITGEIMYVDGGFNTTALGNAQPMA"
     gene            complement(7443..9344)
                     /locus_tag="PLH85_11045"
     CDS             complement(7443..9344)
                     /locus_tag="PLH85_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNB65148.1"
                     /translation="MFDAVRNNKKVVQIFLLLITLPFAFWGVDSYVRNMGKGDELASV
                     GGHAITQAEFQQAMRSQQDRLRASLGDQFNPAMLDSPEARRAVLENMINQRLLGLDAG
                     KHRLLVSDAQMRDTILAVPGFQENGQFSQVRYEALLKAQGMNQAIFESRLRQDLALQQ
                     IIIPLTQGILSGASAERVLGTQIEERTVSEAVLAPPQFMASVKLGEDAAKNFYEANRQ
                     RFEVPAQVRAEYVALDQDAVASQVSVSDAEVRKAYETAADRYRQGEERRASHILIQAD
                     KAAGEAVIKAAREKAEGILKQVKAAPGEFAKLAKANSQDPGSADKGGDLGYFGHGMMV
                     KPFEDAVFALAKEGDTTDLVQSDFGFHIIRLTGIKAAKVRPLEEVKGEIAAELRKKAA
                     SGKLAEMAEGFSNTVYEQSDSLKPAAEKYKLNVQTSDWIVRAAKLPPPFDNERLVSAL
                     FSDDAVKNKRNTDAIDVGGGVLVSARVVEYKPASLRPFEDVKPLIEKRLAADEASRLA
                     VKEGEARLGKLQKGEAVELSWGAPRVLQRGAQDLPPAAAAAVFRVASDKLPGYTGVEV
                     PGAGYVLYRVEKVVRPEAAASDPRLQVVRQQYGRVLAEMDFAAYIAALRQRYDVKINM
                     AALEGNKEK"
     gene            complement(9697..9773)
                     /locus_tag="PLH85_11050"
     tRNA            complement(9697..9773)
                     /locus_tag="PLH85_11050"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9737..9739),aa:Asp,seq:gtc)
     gene            complement(9807..9882)
                     /locus_tag="PLH85_11055"
     tRNA            complement(9807..9882)
                     /locus_tag="PLH85_11055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9847..9849),aa:Val,seq:tac)
     gene            complement(9917..10189)
                     /locus_tag="PLH85_11060"
     CDS             complement(9917..10189)
                     /locus_tag="PLH85_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB65149.1"
                     /translation="MNKSELIDQVSAEADISKAAVGRVLDATFAAVKLALKKGGSVTL
                     VGFGTFHVGKRAARTGRNPRTGAAIKIKSAKIPKFRPGKALKDALN"
     gene            complement(10648..10917)
                     /gene="rpsT"
                     /locus_tag="PLH85_11065"
     CDS             complement(10648..10917)
                     /gene="rpsT"
                     /locus_tag="PLH85_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNB65150.1"
                     /translation="MANSAQARKRARQAIKARAHNMSLRSRLRTAIKAVRKAIDGGDK
                     SAAQQVFVASQRTIDSVADKKIIHKNKAARHKSRLSAAVKAMAAA"
     gene            11058..12596
                     /gene="murJ"
                     /locus_tag="PLH85_11070"
     CDS             11058..12596
                     /gene="murJ"
                     /locus_tag="PLH85_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNB65151.1"
                     /translation="MNLLRALATVSGMTLLSRILGFIRDFVVARAFGAGLATDAFFVA
                     FRLPNLLRRMFAEGAFSQAFVPILAEYKNRRGPDETRTLVDHVASALGVVVLIVSILG
                     ALGAPAIIYATAPGFSSDAEKFDLTVQLTRITFPYIFFMALVALSGGILNTWSRFAIP
                     AFTPVLLNLSFIGMALFAAPYFDPPVVALGWAVFIGGLAQVLLQIRPLARIGMLPRFS
                     LSFADPGVRRILKLMAPAILGVSVSQISLLINTVFASFLPSGSVSWLYYADRLMEFPA
                     GLLGVALGTILLPSLSKMHADDNRSEFSALLDWGLRLTLMLTLPSALALALLAVPLVT
                     TLFFHGAFKVADVYATRSALMAYSAGLAGLILVKVLAPAFYARQDIRTPVRIALITLA
                     ATQLMNLAFVGVFRHAGLALSIGLASCINAGLLFRGLRLRGIYLPQPGWRAFFLKLLA
                     ALTVLGGGLWLTMGADELWLTASGLDRVTRLTALVIGGIIIYFATLFALGFRVSDFHR
                     RGAT"
     gene            12656..13285
                     /locus_tag="PLH85_11075"
     CDS             12656..13285
                     /locus_tag="PLH85_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNB65152.1"
                     /translation="MHLRSSSFADGAVISGVHTFCVPAAEGHVCLGANRNPHLAWREA
                     PAGTRSFVVLCHDPDVPSRGDDVNQEGRLVPADLPRVDFFHWVLVDLPETQMEIAEGS
                     HSSEVTPNGKPGPDRPDGSRHGLNDYTGWFAGDAQMKGQYFGYDGPCPPWNDSIRHRY
                     VFTVYALDVHRLTVTGAFTGQDVRNAMHGHILAQASLTVTYSLNPTIPA"
     gene            complement(13319..14569)
                     /locus_tag="PLH85_11080"
     CDS             complement(13319..14569)
                     /locus_tag="PLH85_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNB65153.1"
                     /translation="MNGNLLYAQSGGVTAVINASAAGVITAARRVPQQIARVLAARHG
                     IMGVLQERLVDTSALTDADLACLGRTPGGAFGSCRLDLDPVERNPAQYDRLFAVLAAH
                     DVRALLYNGGNGSMETCRQIAAAAEERRFPLTVVGVPKTVDNDILLTDCCPGFGSAAK
                     YLATSVREVGLDLAAMTTGGGRAFVLEVMGRNAGWLAAACALASDGEGAAPHVILLPE
                     VPFDEEAFLAKVAEVVARHGACAVVVAEGLHDAAGEPIAMQERSAKGYVQLGGAGEAI
                     AERIHRRLGCKVHFAVADYLQRAARHLASATDATQAGAVGEAAVAAALRGEGGMVAIE
                     RLGDVPYRWRTRIVPFDAVANLERRLPPEFIRPDGFGVTSAFRSWCRPLIEGEDWPAF
                     RDGLPEYFHLRLPLLTQRLPIWAA"
     gene            complement(14846..15499)
                     /gene="adk"
                     /locus_tag="PLH85_11085"
     CDS             complement(14846..15499)
                     /gene="adk"
                     /locus_tag="PLH85_11085"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNB65154.1"
                     /translation="MRLILLGPPGAGKGTQANYIKEKFGIPQISTGDMLRAAVKAGTP
                     LGIEAKKVMDAGGLVSDDIIIGLVKDRLKEADCQAGYMFDGFPRTIPQAEAMKAAGVP
                     IDFVLEIDVPDEEIIGRMSGRRVHVASGRTYHVMFNPPKVDGKDDETGEALIQRDDDK
                     EETVRKRLDVYHSQTKPLVAYYSEWAASGDAAAPQYRKIGGLGKVEEIRDRAFGALK"
     gene            complement(15547..16320)
                     /gene="kdsB"
                     /locus_tag="PLH85_11090"
     CDS             complement(15547..16320)
                     /gene="kdsB"
                     /locus_tag="PLH85_11090"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNB65155.1"
                     /translation="MGFRIVIPARYASSRLPGKPLLDIAGRPMILRVLDQARLAGADE
                     VWVATDHADIAERVEAAGGQVILTGRELPSGTDRLAEVATRLGWDDDTVVVNVQGDEP
                     LIPPSLIADAAATLSADAEAAIATACHPLASAEEFFNPNVVKLVLDARGRALYFSRAP
                     IPWARDAFAQQRDALPAGFPAYRHVGLYAYRAGFLKRYAALAPSPLEQWEALEQLRAM
                     AHGFPIRVMVLDHAPPGGVDTAEDLERVRRVFDVASVSR"
     gene            complement(16324..16512)
                     /locus_tag="PLH85_11095"
     CDS             complement(16324..16512)
                     /locus_tag="PLH85_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNB65156.1"
                     /translation="MDPRLLEILVCPITKGPLDYRKDKQELWSVSARLAYPIRDGIPV
                     MLEEEARPLTDDEVAQVR"
     gene            complement(16493..17503)
                     /gene="lpxK"
                     /locus_tag="PLH85_11100"
     CDS             complement(16493..17503)
                     /gene="lpxK"
                     /locus_tag="PLH85_11100"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNB65157.1"
                     /translation="MPRRAPAFWSRRGAVAWALMPLSLLFGAVAGLRRGLFRNGWLRT
                     ERLPAPVIVVGNIAVGGSGKTPVTLWLVEHLKAAGRRPGIVSRGYGGHVEGVAEVPPD
                     GDPSHFGDEPVLMAHLAGVPVWVGRDRPAAGRALLAAHPEVDVLVCDDGLQHYRLERD
                     VEVVVVDQATLGNGLRLPAGPLREGVTRLARADLVLAHGALAPSVRRACGTTPVFEFH
                     LAGAAFERLSDRGERRPPSALMGRRLRALAGIGRPERFFAQLEAMGLAVERCPLPDHH
                     VFTPADVALEGVDALVMTEKDAVKCAPFAPHESWVWPVRAHVPSRAAELILEKIDGSP
                     SA"
     gene            complement(17538..17960)
                     /locus_tag="PLH85_11105"
     CDS             complement(17538..17960)
                     /locus_tag="PLH85_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB65158.1"
                     /translation="MNFRRGQSREEPEINLIPMIDVLLVIIIFLMLTTTYSRFAGLEI
                     NLPTADAQASAEKTVELNVAVTATGDVVVNKRAVSGTSVQAIAAALQALVPQGAKDPV
                     VIINADAKATHQRVIDVMQAAQQAGLVHISFATQAAQR"
     gene            complement(17970..18575)
                     /locus_tag="PLH85_11110"
     CDS             complement(17970..18575)
                     /locus_tag="PLH85_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB65159.1"
                     /translation="MFAILQAAGWPIWPLLFASIVAVALIIERLITLRRSKIIPDGLL
                     DKVVAELRQTGATPEMISRVALSSPLGRVFAAGLRNVRSPREVMKESIEEAGRAVTHD
                     LERFLTTLGTIASISPLMGLFGTVVGMIEIFGSQAPSGSNPQQLAHGISIALYNTGFG
                     LVIAIPSMIFWRHFRATVDSLVIEMEQQAIRLVEVVHGERR"
     gene            18875..20236
                     /gene="xseA"
                     /locus_tag="PLH85_11115"
     CDS             18875..20236
                     /gene="xseA"
                     /locus_tag="PLH85_11115"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNB65160.1"
                     /translation="MNPPENHPATPDSRVISVADLNRRAREVLEGTFPLQWIAGEVSS
                     LTRAASGHVYFTLKDDEAQVRCTLWRNRAQLLPFRLEHGMQVEIRAVVTLYEARGDFQ
                     LNVDTVRRAGQGNLYEAFLRLKARLEAEGLFASERKRPLPTYPRGIAVVTSPQAAAWQ
                     DVLAALARRAPHVPICLYPTAVQGSDAGARIAAALRLAGTRAVQDSTDVLLLVRGGGS
                     LEDLAAFNDEAVARAIRDCPLPVVCGVGHETDVTIADFAADLRAATPTAAAELASAGF
                     HALHERLSLLAGRLNRAMERRLTHAAQRLDRTALRLRHPRERLREAALRLDHLARRLQ
                     TAGHEPPAHGRNRLDALRLRLSARRPDASRARDRLESLAGRLQRALRIQLERRRARVD
                     ALTEHLTHLDPRSVLARGYTITRDARGRVLRNAADAVPGQDIRVEFHDGEADARVMAR
                     RLR"
     gene            20339..>20876
                     /locus_tag="PLH85_11120"
     CDS             20339..>20876
                     /locus_tag="PLH85_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNB65161.1"
                     /translation="MAHTLPELPYAKNALAPAISEETLEFHYGKHHQAYVTNLNNLIP
                     GTEYESLSLEEIVKKAPAGGIYNNSAQVWNHTFFWNSMKPNGGGAPSGALADAINAKW
                     GSFDDFKKAFQASAVGNFGSGWTWLVKKADGSVDIVNMGAAGTPLTTGDTALLCIDVW
                     EHAYYIDYRNLRPKFVETF"
BASE COUNT         3714 a         7403 c         6237 g         3522 t
ORIGIN      
        1 gccgacgtgg tgaacaagat ccgccgctcg cccaatgccg ccgagttcat ccgcctcttc
       61 ggcagcgaca tcctcgaccg ccccgacgac gccttcgccc gcctcctcct cgcccttcag
      121 caataccaga ccgaagaccc ggacttcgcg cccttcacca gcaagttcga cttctggcaa
      181 gccggcaagg cccagctcac tgctcaggag ctgcgtggct gggccctctt caacaacccg
      241 gtcaagggca attgcgccgc ctgccacatc agcaccggcc agaacggcgt cccggccctg
      301 tttaccgact tcacctacga caccctcggc gccccccgca acatggacat cgccgccaac
      361 gcggatccgg cctactacga cctgggcctg tgcggcccga gccgcagcga cctggctgcc
      421 cgcaccgacc tgtgcggcgc cttccgcgtc cccagcctgc gcaacgtggc cgccaccgcc
      481 ccctactttc acaacggcag cctcaagacc ctcaccgacg tggtgcgctt ctatgtccgc
      541 cgcgacacga atcccgagga gtggtacccc accgacagcg ccggcgtcgt ccagaagttc
      601 aacgacctgc ccgaggctta ccgccgcaac gtgaacacca ccgaagtgcc ctacaaccgc
      661 aagcccggcg acagtcccgc cctcgacgcc agcgagatcg ccgacctggt ggccttcctc
      721 aacaccctca ccgacggcta cacgccctga gccgccgcat cgcttccctg catcccggag
      781 atcctcccat gaagaccaca ccgctcagcc tcgcgctcgc cgcctgcttc ctcagcagcg
      841 gcgctcaggc cgccaccgac gccgaactcc tcgcccgcat cgaccgcctc gccgccgaac
      901 tggaggccgt caaggccgaa ctgcgcagcg tcaaggccca ggccaccccg cctgcgccca
      961 tcaccacgac agcggccacc accccagccg ccactgtgcc ggtccccgcg gctcccggtg
     1021 cgtacgccca gaccgccagc gccagcggcg ccaacccgac cagcctcttc ggctatggcg
     1081 aggtcaatta caatcgcccc ttccgcaaca gcagcgacac ccgcatggac gtgcgccgcg
     1141 ccgtcatcgg tgtcacccac caggcctccg aacgcacccg catcgtcggc gaattcgagt
     1201 ttgagcacgc cgtcgcctcg gcaaccgatt ccggcgaggt ggaagtcgag cagctctacg
     1261 tggaacaccg cctcaacgac aacgtgggcc tcaagggcgg cctcttcctg atccccctgg
     1321 gcaccctcaa cctgagccac gagcccctgt cctactacgg tgtggaacgc aactttgtcg
     1381 agaccgccat catccccacc acctggcgcg aagtcggcct ctccaccttc ggcgtcaccg
     1441 acgaaggcct gcgctgggac gtgggcctca ccagcggctt tgacctgaac aagtgggaca
     1501 agaccagtga cgaaggccgc accgaaggcc ccctggcggc catccaccag gaaggctccc
     1561 aggcccgagg ccatgacctc tccgtgtacg ctgccctcga ctggcgcggc acgcccggcc
     1621 tggccgtcgg cggcggcgtc ttcagcggca aagcctccca caaggtcaac gacccctcca
     1681 ccgccaacgc cggtgtatac gcccctggtg cccgtgtaac cctgtgggac acccacgccc
     1741 gctggacgcc cggcaagtgg gatctggccg ccctctatgc ccgcggcagc atcagcgaca
     1801 ccgatcagct caacgtccag ctcgccggcg gcacccccgt gcccaagtcc ttcttcggct
     1861 ggtacaccca ggccgcctat cgcctgtggg aaaacggcga atacaagctg agtcccttcg
     1921 tccgtcacga acgcttcaac accggcgcca cctacgcagc gatgcccgcc ggcctggaga
     1981 tcgacccgct gcgcaccgag aaggtcctca cagccggcgc caacttccag gtgggtggca
     2041 atgtggtcct caaggccgac gtccagcgct tcgtcgtcga tcgcagccgc gaccgcctca
     2101 acctgggcct tggctaccag ttctgacctg gcctgccggc acccccggac gcccggacgc
     2161 cgcttcggcg gcgcccgggc gccgtcacgt tcacccaagg gcttgaaccg atgaaacatt
     2221 cccgacatga gtggccggtc gtcccggcgt ccctactgat cgccgccggt gcgctcgcca
     2281 ccttccaggc cccccacgcc atcgccgccc agtacatgag cgcggaacag gcccagaaga
     2341 cggccttccc cgacgccgac cgcttcgacg tacgcccgct gcgcctcacc ccggagcagc
     2401 tgcgcaccct cgacggcgtg gccccggtcc gccaacgcgg tgaattgcgc cagatcctcg
     2461 cctacgccgg tccccgcctg ctgggcacgc tgtacgtgga cgatgtcatc ggcaaggtgg
     2521 agtggatcac ctacgctctg gccatcaatc cggacggcag cgtgcgcagt ctggacatcc
     2581 tcgaatatcg cgagacccac ggtttcgaag tgcgcaccgc gtcctggcgc aagcagttcg
     2641 tcgggcaccg cagcgacacg ccgctgcatt tcggcgacga catcaagaac atcagcggtg
     2701 ccaccctgtc ctgcgcccac ctcaccgcgg gtgtgcagcg cctgctcaag ctgcacgccc
     2761 agttggccag cgccgaacga tgagcgacgg cccgaccgca cgccgggccc aggcctggct
     2821 cggaacctat gtcgtcatgg aagcgacagc cgccgagcag cgtgtcgccg acgcggcggt
     2881 tgcagccgcc ttcgacgctg tcgccgacgt tcaccgcctc atgagcttcc acgcccagga
     2941 cagtgatctg gccaacctgc acgccgacgc ctggcgccag cccgtgaccg tctcgcccca
     3001 cacctggcag gtcatcctcc acgcccagac cgtcgccgcc gccagcgacg gcgtcttcga
     3061 ccccaccgtc gcgcctgccc tggtggccag cggtgccttg ccggccccca ccggtccgcg
     3121 tccggacccg accgccacct gggccgacgt cgaactgctc gatggccagc agatcgcctt
     3181 ccgtcgcccc ctgtggctgg atctgggcgg catcgcaaaa ggctacgcgg tggaccgggc
     3241 cgtacacatc ctgtgcgagt acggtgccga cagcggtctc gtcaatgcgg gcggcgacct
     3301 gcgcgtgttc ggacggcatc gcacgcgcgt cccggtggcc gtccgccacc ccgcccagcc
     3361 gtcccagacc ctgcccctcg gcagcctcgc ccagggtgcg gtggccggct ccggcgacta
     3421 ctttgccgcg atgccggcgg aacaggcctc ccccatcgtc gcgccgggcc gaggcctccg
     3481 tcccgctggt cgcaccgccg tggtggtggt cgcggatgaa tgctggtgcg ccgatgccct
     3541 gaccaaggtg gtaagcctga tggagagcgc ctccagcccg gtgctgcgca tgttcaacgc
     3601 cactgcagcc ctcctcgatg cgcatggtac gctgcgcgcc atgccgggct tcatggaacg
     3661 cctcgggcat gccacccctg aggaacagtc cagccatgca tgagtttccc caccgccccc
     3721 gccacaaggc ggtccgcccg ggccggcggc aacgccacag cctgtatgcc gtcgcggccg
     3781 tcctgatggt cagtggtctg gtgtggctcg tcgcccatta cgcgctgccg ctcccggatg
     3841 atgctgcgcg ccatccggcc gaaccctggg ccatgcggat ccacggcgcc gcaacgctgc
     3901 tgggcatggc cgtactgggg gccttatgga gccatcacgt gctgccggcc tggcgggcgg
     3961 gcgaaaagcg cgcgaccgga ctgcctctgc tcggaatctg gctggccctg gccgtcagcg
     4021 gctatggcct gtattacctg gcggaggaat cctggcggga tgctgccgac acgctgcacg
     4081 tgagcgcagg actggcgatg cccctggccc tggccagcca tgtctggatg cagggccgac
     4141 gacaaaaaaa gggggagcgg ccttcttcag gaccgcaccc cctgtcaccg caaagcaagg
     4201 agacaggctg atcgcacccg caacgatgcg acgcctgcgt catatattag ccccttgccc
     4261 gtcgaatcgc cacccgattg tcattcattg ccctgttccc ggcggcgata atgcgccacc
     4321 gccggcaacg caaggactac cagcaggatg agcagcacgg ccagcggcca gaccacgggt
     4381 cggttccatt ccgcccgcat cgctgatcgc gtggtcacgt caatgcgctg gtacttgagc
     4441 gtgctgcgca caatcttgcc cggcttgcgg ttgctcaccc agccgtgctg gagcgtgtag
     4501 gccttttcgt agaagcccca cacccacggc gcgtcgtgct gcaggatcgc cagcatgcgc
     4561 gcgatgaggg ccagccgttc cgggctgtcg ggcatggcct tcatgcgctc gaacaggcgg
     4621 tcgtattccg gattggcgta gttggccgcg ttctcgccct ggttcttcac cttgctctgg
     4681 gggccataga gcaggaacag gaagttctcc ggatcggggt aatccgcgtt ccagccccag
     4741 tagaagagct gcgcgttgcc cttgcggatc ttgtcctgga aacggttgta gtcggtggcc
     4801 cgcaccacga gctgcacatc gagcttgttc agctgcttgg tcagccagtc caccgtggat
     4861 ttgtcgccca tgcccgaccc ggtggtatcc agattcacca ccagtggctc gccggtcttt
     4921 gcgtcgcgcc cattggggta gcccgcctca gcaagcagac gccgggcgtc gtctaccgat
     4981 ttgcgccgcg ccttgccgtc gacccagtcg tacacgtagg gattgatgcc cgcctgatcc
     5041 acccggtagc ccgtgatgcc cggcgggatc gggtgcatcg cgggtacacc gcggccattc
     5101 tggaagaccg agatgaactc ctcctggtca atggcaatgg aaatcgccag ccgcagcttg
     5161 cgtgcccgct ccgaatcgcc gcccaccagc gggtcgagca tgttgaagcc catgtacatc
     5221 accgtcggcg ccaccgaagt cagcagacgg atgcccttcc tggccatctc gtcgctgagg
     5281 gccacatcgc cctgggcacc catctgcacg gcctgatcga agttgtcgga ggacacgcct
     5341 gaagcgtcgt aatagccctg caggaacttg ttccagtacg gaatgctttc cttctcccgg
     5401 gtgaagacca ccgtgtcgac gaaggggatc accttgccgc aatcctcgag aaagcctgcc
     5461 gcacgatcat ccggttcacc ctcgcacgga tagcgttcac cacggaaatt cgggttgcgg
     5521 gccagcacca tgcgggcatt gggattgttc tcgaccagca tgtacggtcc ggtgcccacc
     5581 ggccagctgt cgagggtcag gttgttgtct gccatgcccg gctgggcaaa gaagcggtcc
     5641 gcctcgaaag gcaccggcgc gaagaagggc atggccagcc agtaaggaaa ctggggatag
     5701 gtgccctgca gatgcacccg gtaggtgtag cggtccacca cctcgacgcc gggcaggtcg
     5761 tagcggcgca gatcgatcca ggcgcccttg tcggcgcgcc cccgctcctc ggccaccaga
     5821 cgcgcattca ggtccttcag cccgacgatg tagtctccca tcagctcgag caccggggaa
     5881 tgcagacgcg gatgggccag acgcttgatc tgatagacga aatcgtcggc ggtcagctcc
     5941 cgcgtgccgg attctggaaa atcggacggg cggcggcgct gcgcgatgtc gctgcgcgac
     6001 agggccacat agcgggcctg ccccgcagca tcgacggcaa acgccggatg aggctgataa
     6061 cggacgccgg gacgaatcct gatctcgtag gtgctgcggg cgacccgtgc cggatcggca
     6121 tcgggcggca gttcgtgccc cgcccgatca tactgacgca ggacaggaag cgtcgcggcc
     6181 gtggccggtt caagctggta ggggcgcttg aggtaatggt actggagggg cggttcgtag
     6241 atctgataca ggaaggtcgc ctcgtcctca ctgtaggact ggaccgggtc cagatgtttc
     6301 gggcgctggg tgaaggccga atacaggatg ttctgccctc gctcggccac tggatacgga
     6361 tcgttccaga ccccgtcgca gccagcaacg gcaagaccca gcagggccga aacaaaaagg
     6421 acgatcatgc gatgcatggc cgcgagtgta ccggaaggtc cgcactccgc ggcagccacg
     6481 cgagggcgca agcatccgtt ataatcggcg ccggattttc atcggagaca cctttcatgg
     6541 gctttcttgc cggcaagcgc attctcatca ccgggctgct gtccaatcgc tcgatctcct
     6601 acgggatcgc caaggccatg cgccgtgaag gagccgagct tgccttcacg taccagaacg
     6661 agcgcttcaa ggagcgtgtc gccaagttgg ccgccgaatt cgacagcacg ctgatttttc
     6721 cgtgcgatgt gcagaacgat gccgaaatcg ccagcctctt cgagcaactc ggcacccaat
     6781 gggacggact ggacggactc gtccactcca tcgcttttgc gcccggtgaa gccctggaag
     6841 gtgatttcct cgacggtctg acccgcgaag cctaccgcgt cgcccatgaa gtgagcgcct
     6901 acagcttccc cgccctcgcc aaggctgcgc ggccgatgat gaagggccgc aaggggtcgc
     6961 tgctgaccct gtcttacctg ggcgccgtgc gcaccatgcc caactacaac atcatgggcc
     7021 tcgccaaggc cagcctggaa gcctcggtgc gctacatggc ggtatccatg ggccctgaag
     7081 gcacgcgggt caatggcatc tccgccggcc cgatcaagac cctggcggcc tccggcatcg
     7141 gcagctttgg caagctgctg gccttcaacg agcacaacgc cccgctgcgc cgcaatgtga
     7201 ccatcgagga agtgggcaat gccgcggcct tcatgtgttc cgacctggcg agcggcatca
     7261 ccggtgagat catgtacgtg gatggcggct tcaataccac cgccctcggc aacgcccagc
     7321 ccatggcctg aacccaccgc accgcatcag gacgaaaggc ccgcactgct gcgggccttt
     7381 ctcatttcag gcatgcctcc gtggcggcgc ccggaacgaa cgcgcgccac aatgaagccg
     7441 actcacttct ccttgttgcc ttccagcgcc gccatattga tcttcacgtc gtaacgctga
     7501 cgcagggccg cgatgtaggc cgcaaaatcc atctcggcca gcacccgccc gtattgctgg
     7561 cgtaccacct gcaggcgcgg atcactggca gcggcctccg ggcgcaccac cttctcgacg
     7621 cgatacagga catagcctgc acctggcact tcgacacccg tgtagcccgg cagcttgtcg
     7681 gacgccaccc ggaacactgc cgcggccgcg gcaggcggaa gatcctgcgc accgcgctgc
     7741 agcacgcgcg gcgcgcccca gctgagctcc acggcctcac ccttctgcag cttgccgaga
     7801 cgggcctcgc cctccttgac ggccagccgg gaggcctcat cggccgccag acgcttctcg
     7861 atgagcggct tcacgtcctc gaacggacgc agactcgccg gtttgtactc cacgacacgc
     7921 gccgaaacca gcaccccacc gcccacgtcg atcgcatccg tattgcgctt gttcttgacg
     7981 gcatcgtccg agaacaaggc ggacaccaga cgttcgttgt cgaacggcgg tggcagcttg
     8041 gctgcccgga cgatccagtc gctggtctgg acattcagct tgtacttctc ggctgccggc
     8101 ttgaggctgt cggactgttc atacaccgtg ttgctgaaac cttcagccat ttcagccagc
     8161 ttgccggagg cggccttctt gcgcagctca gccgcaattt cgcccttcac ctcttcaagc
     8221 ggacgcacct tggcggcctt gatgcccgtc agccggatga tgtggaagcc gaaatccgac
     8281 tggaccagat ccgtcgtatc gccctccttg gccagggcga acaccgcgtc ctcgaagggt
     8341 ttgaccatca tgccgtggcc gaagtagccc agatcgcccc ccttgtccgc agaaccggga
     8401 tcctgggaat tggccttggc gagcttggca aactccccgg gcgcagcctt gacctgcttg
     8461 aggatcccct cggccttctc gcgtgccgcc ttgatgacgg cctcgccggc cgccttgtcg
     8521 gcctggatca ggatgtggct ggcccgccgc tcttcgccct gacggtaacg gtccgcggcc
     8581 gtctcatagg ccttgcggac ctcggcgtcg ctgacgctca cctggctagc gacggcatcc
     8641 tggtcgaggg ccacgtattc ggcgcggacc tgggccggca cctcgaaacg ctgcctgttc
     8701 gcctcgtaga aattcttcgc cgcgtcttcg cccagtttga cggaggccat gaactgcggc
     8761 ggcgccagaa ccgcctcgct caccgtacgc tcttcaatct gggttcccag aacccgttcg
     8821 gcggacgctc cggacaggat gccctgggtc aacggaataa tgatctgctg gagcgccaga
     8881 tcctgacgca ggcgcgattc gaagatcgcc tgattcatgc cctgggcttt caggagcgct
     8941 tcgtagcgga cctgggagaa ctggccgttt tcctggaaac ccggcacggc cagaatggta
     9001 tcccgcatct gcgcgtcgct caccagaaga cggtgcttgc ccgcatccag accgagcagg
     9061 cgttggttga tcatgttttc cagcaccgcg cgacgggctt ccggactgtc cagcatcgcc
     9121 ggattgaact gatcgcccaa cgaggcccgc agacggtcct gctggctgcg catggcctgc
     9181 tggaattcag cctgagtaat tgcgtgcccg cccacggagg cgagttcatc ccccttgccc
     9241 atgttccgga cataggaatc cacgccccag aacgcaaagg gaagcgtgat gaggagcagg
     9301 aagatctgga cgaccttctt gttgttgcgt accgcgtcga acatgctgaa gaatcctctt
     9361 ggctgcggtg agcaggcgca tcgggtggct gaaaatgcaa aaaaggcgaa cttgcgttcg
     9421 cctcaatctg aacgaaggat ccggttcatc accctgagca agctccatca cgtgcgcaac
     9481 acatgaacgg gtctgagcct tggtcaagac cccggaccgt cggacgaaaa gcccgtcgaa
     9541 tatagcaagc gcccgtcggg aagagcaagc ccggacgcac gtaccaacac cccgccatcg
     9601 gcgatccgaa gcccccgaaa cgacaaaggc tgcagggagc agccttttgc gcaacggtgt
     9661 tcaggaaacg accggaaaca actcacttgg gcatcatggc ggagtggacg ggactcgaac
     9721 ccgcgacccc cggcgtgaca ggccggtatt ctaaccgact gaactaccac tccttgtact
     9781 tctaactaca aacttcgaat cctggctggt gggtgctgac ggggtcgaac cgccgacatt
     9841 cgccttgtaa gggcgacgct ctaccaactg agctaagcac ccgctgcaca cggactatcg
     9901 cccgcactac agcccatcag tttaatgcat ccttgagcgc tttgccaggc ctgaattttg
     9961 ggatcttggc ggacttgatc ttgatggccg ccccggtacg cggatttcgg ccggtccgag
    10021 ctgcgcgctt gcctacatga aacgtaccga agcccaccag cgtcaccgag ccaccttttt
    10081 tgagcgccag tttgacggct gcaaaggtcg catccagcac acgccccacc gcagccttgg
    10141 aaatatcggc ttccgcggaa acctgatcga tcagttcgga tttattcact tcagtcccct
    10201 tttgatgtgg atcgtgcgaa cgaaaaaacc gaaatctggc gaagcagggc gagcgacgtg
    10261 acgaattgcc ctaattggat cttgcgagtt ttatagcgcg cgaaaaaacg tcgtgtcaag
    10321 gcacgacggc gcacgcgcgc ttgaattgca cgaaaaacgg acaagacccg cagtaggtat
    10381 tgttaatttc tgcaccgaac ccggccccaa tacggatttg cttcgtcacc ccgagtgacc
    10441 gatcgccccc ccgacacgcc gggccccgcc gcgccacgcg acagaccggg cacgggccgg
    10501 ccgaagggag tgacccggca gcacgtgact tccgccagcg ccgatccacc ggatctgacc
    10561 tcacggatgc atccgcaccg ggaccacaaa acaaaatggc acaagcttcc gcttgtgcca
    10621 ttccgaaaac cgaagcttgc gcgaggatca ggcagcagcc atcgccttga cggcagcaga
    10681 caggcggctc ttgtgacgag cggccttgtt cttgtggatg atcttcttgt ctgcaacgct
    10741 gtcgatggtc cgctgggacg caacgaaaac ctgttgcgca gcagacttgt caccgccgtc
    10801 gatcgcctta cggactgcct tgatggcggt gcgcagacgg gaacgcaggc tcatgttgtg
    10861 ggcgcgggcc ttgatcgctt gacgggcgcg cttgcgtgct tgtgccgaat tggccatgtt
    10921 tatggtccca acagaatcca ataaagcgtt cgattttaga ggaagcattc tagcaatgca
    10981 agcttttatt cccgattgca ttgccgatcc cgaccggcag tcgctaccat gggcactttt
    11041 cccatcgctg gcccgccatg aatctccttc gcgcccttgc cactgtcagc ggtatgaccc
    11101 tgctttcacg gattctcggc ttcatccggg attttgtggt cgcgcgggcc tttggtgcgg
    11161 gactcgcaac cgatgccttc tttgtcgcgt tccgcctgcc gaatctgctg cgccggatgt
    11221 tcgccgaagg cgcgttttcg caggccttcg tgccaatcct cgccgaatac aaaaaccggc
    11281 gcggacctga cgagactcgc accctcgtcg atcacgtcgc cagcgccctc ggcgtcgtgg
    11341 tgctgatcgt ctccattctg ggcgctctcg gcgcgcctgc catcatctac gccaccgcgc
    11401 cgggtttttc atcggacgct gaaaaatttg acctgacggt ccagctcacc cgcatcacct
    11461 ttccctacat cttcttcatg gccctggtgg ccctatccgg cggcatcctc aatacctgga
    11521 gccgcttcgc catcccggcc ttcacgccgg tgctcctcaa cctgtcgttc atcggcatgg
    11581 ccttgtttgc cgcgccttac tttgacccgc ccgtcgtggc gctgggctgg gcggtgttca
    11641 tcggagggct cgcccaggtg ctgctgcaga tccgcccgct ggctcgcatc ggcatgcttc
    11701 cgcgcttttc cctgagcttt gccgatccag gcgtacggcg catcctgaag ctgatggcac
    11761 cggccatcct gggggtgtcg gtcagccaga tctcactgct catcaacacc gtcttcgcat
    11821 cctttctacc cagcggcagc gtgtcatggc tgtactacgc tgatcgcctg atggagtttc
    11881 cggcaggcct gctgggcgtg gccctcggga ccatcctcct gcccagtctc tccaagatgc
    11941 acgccgacga caatcgatcc gaattttcag ccctgctcga ctggggactg cgcctgacac
    12001 tcatgctgac cctgccctcg gcgctggccc tcgcgctgct ggcggttccc ctcgtcacca
    12061 ccctgttctt ccacggcgcc ttcaaggtcg ccgacgtgta tgccacccgc agcgccctga
    12121 tggcctacag tgccgggctg gccggactga tcctggtaaa ggtccttgca ccggccttct
    12181 acgcccggca ggacatccgc acaccggtgc gcatcgccct catcaccctg gccgcgacgc
    12241 agctgatgaa ccttgcgttc gtgggcgtct tccgccacgc cggcctcgcc ctgtcaatcg
    12301 gactggcgtc gtgcatcaat gccggtctgc tctttcgagg cctgaggctc cgcggtatct
    12361 atctgccgca accgggctgg cgtgccttct tcctcaagct gctcgccgcc ctgacagtac
    12421 tcggcggcgg cctgtggctg accatgggcg ccgacgaact gtggctgaca gccagcggcc
    12481 tcgaccgggt gacccggctg acggcactgg tgatcggcgg gatcatcatc tatttcgcca
    12541 ccctctttgc gttgggcttt cgcgtcagcg atttccatcg ccgcggcgcg acctgagccc
    12601 cgcactcacc acccgctgtg tagaatctcg cgaatcattc gggggagacc acatcatgca
    12661 tctgcgcagc tccagttttg ccgacggcgc cgtgatttcc ggcgtccata cgttctgcgt
    12721 tcccgctgcc gaaggccatg tctgcctggg cgccaatcgc aacccgcatc ttgcgtggcg
    12781 cgaagcccct gccggtaccc gctccttcgt cgtgctctgt cacgatccgg acgtaccgag
    12841 ccgcggtgac gacgtgaatc aggaaggccg cctggttcct gcggatcttc cccgcgtgga
    12901 tttcttccac tgggtgctcg tggatctgcc cgaaacccag atggagatcg ccgaaggcag
    12961 tcattcgagc gaagtgactc cgaacggcaa gcccgggccg gatcgccccg acggcagccg
    13021 tcacggactg aacgactaca ccgggtggtt tgccggggac gcgcagatga agggccagta
    13081 tttcggctac gacggtccct gcccgccgtg gaacgattcc atccgccacc gctatgtatt
    13141 cacggtctac gcgctggacg tgcaccgcct gaccgtgact ggcgccttca cggggcagga
    13201 cgtacgcaat gcgatgcacg gccacatcct cgcccaggcc agcctgaccg tgacatacag
    13261 cctgaacccc accatccccg cgtgatcatg tcggcctgac gttccgcccg cacccttgtc
    13321 acgctgccca gatcggcagg cgctgcgtga gcaggggaag gcgcagatga aaatactccg
    13381 gcaagccgtc tcggaatgca ggccagtcct ccccttcgat cagcggccgg caccagctcc
    13441 ggaacgcaga cgtcacgccg aatccatcgg gtcggatgaa ctccggcggc aggcggcgct
    13501 ccaggttcgc caccgcatca aagggcacga tccgggtacg ccagcggtaa ggcacatcgc
    13561 ccagacgctc gatggccacc atgccgcctt cgccacgcag cgccgccgcc acggccgcct
    13621 cgccaaccgc gccggcctgc gtcgcatcgg ttgccgaggc cagatgccgc gccgcccgct
    13681 gcagatagtc cgccaccgcg aaatgcacct tgcagccgag acgacggtga atacgctccg
    13741 caatggcctc ccctgcacct ccgagctgaa catagccttt ggcagagcgt tcctgcatgg
    13801 caatcggttc gccggcagca tcgtgcaagc cctcagcgac gacgacagca catgcgccat
    13861 ggcgggcaac gacctctgcg accttcgcga gaaaggcctc ttcgtcaaaa ggaacttcag
    13921 gcagcaggat gacatgcggc gcggcgccct ctccatcgga cgccagcgca caggccgccg
    13981 caagccagcc tgcgttgcgc cccatcacct cgagcacgaa ggcacgccca ccgccggtgg
    14041 tcatggccgc cagatcgagc ccgacctccc gtaccgacgt ggccaggtac ttggcggccg
    14101 agccaaaacc agggcagcag tcggtcagca ggatgtcatt gtcgacggtc ttgggcacgc
    14161 ccaccaccgt gaggggaaag cgccgctcct cggccgcagc ggcgatctgc cggcaggttt
    14221 ccattgagcc gttgccgccg ttgtagagca aggctctcac gtcgtgcgcg gccagcaccg
    14281 caaacaaccg atcatattgg gccggattgc gttcgaccgg atccagatcc agccggcagg
    14341 atccgaatgc gccgcctggc gtccgtccaa gacaggccag atcggcgtcc gtcaaggcgc
    14401 tcgtatccac cagacgctcc tgcagcacac ccatgatccc atgccgggcg gccagcaccc
    14461 gggcgatttg ctgcggcacg cgccgcgccg ccgtgatgac cccggccgcg gaggcgttga
    14521 tcaccgcagt cacgccgccg gactgggcat acaggagatt tccgttcaca acacactggc
    14581 tcccaggtta tgggacagga ccacgggatg cgggtcttgc ctcagcaaaa gaccacgatc
    14641 gcgagaaaca attccaacgc ggatgctcgc ctcgtccatc atgcaggtca cgtccccgcc
    14701 cgcgacaccc gcagaccgtg cggattcacc gtgatcgcag ccgatcacct gctttgcatt
    14761 cagcacgcag acacgccacc acgcgggcca gcacccaaga aaaagccggc cctcaggccg
    14821 gccttcttga caggacaagc gacgcttact tcagcgcgcc aaacgcccgg tcacggatct
    14881 cttcaacctt gcccagacca ccgatcttgc gatactgggg ggccgcggcg tcaccagaag
    14941 ccgcccactc ggagtagtag gccaccagcg gcttggtctg ggagtgatac acatccagac
    15001 gcttgcggac ggtttcttcc ttgtcgtcgt cccgctggat cagcgcttcg ccggtttcgt
    15061 cgtccttgcc gtccaccttg ggcggattga acatgacgtg ataggtccgg ccggatgcca
    15121 cgtgcacacg gcgaccggac atgcggccga tgatttcctc gtccggcaca tcgatctcga
    15181 gcacgaagtc gatgggcacg ccggcggcct tcatggcttc ggcctgggga atggtgcggg
    15241 ggaaaccgtc gaacatgtag ccggcctggc agtcggcttc cttcaggcgg tccttgacca
    15301 ggccgatgat gatgtcgtca gacacgagcc cgcccgcatc catgaccttc ttggcctcga
    15361 tgcccagcgg ggtgcccgcc ttgaccgccg cacgcagcat gtcgccggtg gagatctggg
    15421 gaatcccgaa tttttccttg atgtagttgg cctgggtgcc tttgccggcg cccggcggtc
    15481 ccaacagaat cagacgcatg ccttaccctc ctctggttat tgtcgctgcg acatggggtg
    15541 gtgaaactaa cgcgaaactg acgctacgtc aaacacgcgg cgcactcgct cgagatcttc
    15601 ggccgtatcc acaccgcccg gcggtgcgtg atcgagcacc atcacgcgaa tggggaagcc
    15661 gtgggccatc gcacgcagtt gctccagtgc ttcccactgc tccagcggcg atggggcgag
    15721 ggccgcgtag cgcttcagga aaccagcccg gtaggcgtac aggcccacat gacgataggc
    15781 cggaaacccc gccggcaacg cgtcccgctg ctgggcgaag gcatcccgtg cccagggaat
    15841 cggtgcccgc gaaaaataca gggcgcgacc gcgagcatcc aggaccagct tgacgacgtt
    15901 cgggttgaag aattcctccg cggacgcgag cggatggcag gccgtggcga tcgccgcttc
    15961 ggcatcggcg gacagcgtgg cggccgcatc ggcgatgagt gagggtggaa tcagcggctc
    16021 atcaccctgc acattgacga ccaccgtatc gtcgtcccag ccgagccgag tggcgacctc
    16081 ggcgagacga tcggtaccgg acggaagttc gcggccggtc aggatgacct gcccacccgc
    16141 cgcctcgacg cgctcggcga tgtccgcatg atccgtcgcc acccacacct cgtcggcgcc
    16201 agccagacgg gcctgatcga gcacgcgcag gatcatgggc cggccggcga tatccagcaa
    16261 cggcttgccc ggcaaacgac ttgaggcgta gcgggccggg atgacgatgc gaaagcccat
    16321 gcctcagcgc acctgcgcga cttcgtcgtc ggtcaggggg cgggcctcct cttccagcat
    16381 caccggaatg ccatcgcgaa tcgggtaggc caggcgagcg ctcacgctcc acagctcctg
    16441 cttgtccttg cggtagtcga gcggcccctt ggtgatcggg cacaccagga tttcaagcag
    16501 acggggatcc atcgatcttc tccagaatca gttcggccgc acgggacggc acatgcgccc
    16561 ggacgggcca cacccagctt tcatgcgggg cgaaaggcgc gcattttacc gcatccttct
    16621 cggtcatcac caaggcatcg acgccctcca gggccacatc ggcgggcgtg aacacgtgat
    16681 gatcgggcag cggacaacgc tccaccgcaa gccccatcgc ctccagctgg gcgaagaagc
    16741 gctccggacg gccgatcccg gccagcgccc gcagacgccg ccccatcagc gcggacggcg
    16801 gacggcgctc tccgcggtcg gacagacgct cgaaggctgc gcccgccaga tggaattcga
    16861 acaccggcgt ggtcccacag gcgcgacgca cggacggcgc cagcgcacca tgggccagca
    16921 ccagatcagc ccgggccagg cgcgtcacac cttcacgcag cggaccggct ggcaggcgca
    16981 ggccgttgcc cagggtggcc tgatcgacaa ccacgacctc cacatcccgc tcgaggcggt
    17041 aatgctgcag accgtcgtca cagacaagca catccacctc cgggtgcgcg gcgagcaagg
    17101 ccctgcccgc cgccgggcga tcccgcccca cccataccgg aacacccgcg aggtgtgcca
    17161 tcagcacggg ctcatcgcca aagtgtgagg gatcgccgtc cggcggcact tcggccacac
    17221 cctcgacgtg cccgccatac ccgcggctga cgatgcccgg ccgacgaccc gcagccttca
    17281 ggtgctcgac cagccagagc gtcaccgggg tcttgccact ccccccgacc gcaatgttgc
    17341 ccaccacgat caccggtgcg ggaaggcgct cggtacgcag ccagccgtta cggaacagcc
    17401 cgcggcgaag accggcgacg gcaccgaaca gcagcgacag cggcatcagg gcccaggcga
    17461 cggcgccccg ccgggaccag aaggcggggg cacgacgggg catggaggcg tcggatcagg
    17521 tcaggcccgc gcgccgctca gcgctgggcg gcctgggtcg cgaaggagat gtggacgagg
    17581 ccggcctgct gggccgcctg catgacgtcg atgacgcgct ggtgggtcgc cttggcatcg
    17641 gcattgatga tcacgaccgg atccttggcg ccctggggca ccagcgcctg cagcgccgcg
    17701 gcgatggcct ggacggatgt cccggacacc gcccgcttgt tgaccaccac gtcgccggtg
    17761 gcggtgaccg ccacgttgag ttccaccgtt ttttcggcac tggcctgagc gtcggcggtg
    17821 ggcagattga tctccaggcc ggcaaaacgc gaatacgtgg tggtcagcat caggaagatg
    17881 atgatcacca gcagcacgtc gatcatcggg atcaggttga tctccggctc ctcgcgggac
    17941 tggcctcggc ggaagttcat gacaggacct cagcgacgct cgccgtgcac cacttcgacc
    18001 aggcgaatcg cctgctgctc catctcgatc accaggctgt ccaccgtggc gcggaagtga
    18061 cgccagaaga tcatgctcgg gatggcaatg accaggccga agccggtgtt gtagagggcg
    18121 atggagatcc cgtgggccag ctgctgcggg ttggacccgc tgggcgcctg ggagccgaag
    18181 atctcgatca tccccactac ggtgccgaag aggcccatca gcggactgat cgaggcaatc
    18241 gtacccaggg tggtcaggaa gcgttccagg tcgtgggtga ccgcccggcc ggcttcctca
    18301 atggactctt tcatcacctc gcgggggctc cgcacgttac gcagcccggc cgcgaagacg
    18361 cgccccagag gcgaactcag ggcgacccgg ctgatcatct cgggcgtggc gcccgtctgg
    18421 cgcagttcgg ccacaacctt gtcgagcagt ccgtcgggga tgatcttgct gcggcgcagc
    18481 gtgatgagac gctcgatgat cagggcgacg gcgacgatgg aagcaaacag caagggccag
    18541 atgggccagc cggcagcctg gagaatggcg aacacgcggg ggactcctgc gatgcggaac
    18601 aagcttcgga atttaacctg cccttctgac aagagcaaga atcccgcgcc cgccgacaat
    18661 ccacaaaatc tgtggataaa tgtgtggata gaaaccgtgt gaaggcgcta accctccagt
    18721 ccataaggac ttttcctgct cggatcaaaa atggggcgac gaacacaatc catacttttc
    18781 aataacttac ggaaaacatg cgaagtcaag cccggcggcc agaatatttt tttatggcgt
    18841 gctccaggtg tggattccgc tagaatccgc gcccatgaat ccccccgaaa atcaccctgc
    18901 cacacccgac agccgcgtga tttccgtcgc cgacctgaac cggcgcgccc gcgaggtgct
    18961 tgaggggacg ttccccttgc aatggatcgc cggtgaggtg tcgtcactga cccgcgctgc
    19021 ctccggtcac gtctacttca ccctcaagga cgacgaagcc caggtgcggt gcacgctgtg
    19081 gcgcaatcgt gcccagctcc tgccattccg gctcgaacac ggcatgcagg tggaaatccg
    19141 cgcggtggtc acgctgtatg aagcccgcgg ggacttccag ctcaacgtgg acaccgtacg
    19201 ccgcgccggc cagggcaatc tctacgaagc cttcctgcgt ctcaaggcgc gtctggaagc
    19261 cgaaggactg ttcgcctccg aacgcaagcg tccgctgccg acctacccgc ggggcatcgc
    19321 cgtcgtcacc tcgccccagg cggcggcctg gcaggacgtg ctggcggccc tcgcccgccg
    19381 cgcgcctcac gtaccgatct gcctctaccc gacggccgtc cagggcagcg acgccggtgc
    19441 gcgcatcgct gcggccctgc ggctggccgg cacgcgggct gtgcaggaca gcaccgacgt
    19501 cctcctcctc gtgcgcggcg gcggcagtct ggaagacctg gcagccttca acgacgaggc
    19561 cgtcgcccgc gccatccgtg actgtccgct gccggtggtg tgcggcgtag gccacgagac
    19621 cgacgtcacc atcgcggact ttgccgccga cctgcgcgcc gccacgccaa ccgccgcggc
    19681 cgaactggcc agcgccggat ttcacgccct gcacgaacgc ctgagcctgc tggccggtcg
    19741 cctgaaccgt gccatggaac gccgcctgac tcacgctgcc caacggctcg accggacggc
    19801 cctgcgtctg cgccaccccc gtgaacgcct gcgcgaagcg gcactgcgcc tcgaccacct
    19861 ggcccgtcgc ctgcagaccg ccggacacga gcccccggcc catgggcgca atcgactcga
    19921 tgccctgcgc ctgcgtctgt ccgcacgtcg ccccgatgca agccgggcac gcgaccgcct
    19981 cgagagtctc gccggaagac ttcagcgggc gctccgtatc cagcttgaac gccgccgcgc
    20041 ccgcgtcgac gccctcaccg aacaccttac ccacctcgac ccgcgcagcg tgctcgcccg
    20101 tggctacacc atcacccgcg acgcccgcgg cagggtcctg cgcaacgcgg ccgatgcggt
    20161 gcccggtcag gacattcggg tcgagttcca cgacggcgag gccgacgccc gcgtcatggc
    20221 ccggcgcctg cgctgacacc gatttgtcgc acagcagcaa ttgtcagtcc gcctcaaacc
    20281 cgactagaat ggtcgggcaa ttctgcccac ccctacaaga atcaccggag aatgacccat
    20341 ggctcacacc ctgcccgaac tgccctacgc caagaacgct cttgccccgg ccatttctga
    20401 agaaaccctg gagttccact acggcaagca tcatcaggcc tacgtcacca acctgaacaa
    20461 cctgattccc ggcaccgagt acgagtccct gtccctcgaa gagatcgtca agaaggcccc
    20521 cgccggcggc atctacaaca actccgcgca ggtctggaac cacaccttct tctggaattc
    20581 catgaagccg aatggcggcg gcgctcccag cggcgccctg gccgacgcca tcaacgccaa
    20641 gtggggctcc ttcgacgact tcaagaaggc cttccaggcg tctgccgtcg gcaacttcgg
    20701 ttccggctgg acctggctgg tcaagaaggc tgacggctcc gtcgacatcg tcaacatggg
    20761 cgccgccggc accccgctga ccaccggcga caccgccctg ctgtgcatcg acgtgtggga
    20821 acacgcctac tacatcgact accgcaacct gcgtcccaag ttcgtcgaga ccttcc
//
LOCUS       DAOQMM010000064        20726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_913_length_20726_cov_55.329737, whole genome shotgun sequence.
ACCESSION   DAOQMM010000064 DAOQMM010000000
VERSION     DAOQMM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20726
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_20726_cov_55.329737"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1248)
                     /gene="tyrS"
                     /locus_tag="PLH85_11125"
     CDS             complement(49..1248)
                     /gene="tyrS"
                     /locus_tag="PLH85_11125"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNB65162.1"
                     /translation="MTEITQALELIKRGSDELLVESELVDKLKSGRPLRVKAGFDPTA
                     PDLHLGHTVLINKLRHFQDLGHQVLFLIGDFTGMIGDPSGKNATRPPLSREQVLANAQ
                     TYQEQVFKILDPARTEVVFNSTWMESLGASGMIRLAAQHTVARMLERDDFSKRFGNQQ
                     PIAIHEFLYPLCQGYDSVALKADVELGGTDQRFNLLMGRELQKNAGQSPQCVLTMPLL
                     EGLDGVNKMSKSLGNYIGIYEPPGEIFGKIMSVSDDLMWRYYDLLSFRSSAEVAGLKE
                     AVQTGRNPRDVKVALAQEIVARFHGEPAAEAALTDFEARFQRNAIPDDLPEVAISGGE
                     TGLSVVAVLKQAGLTGSTSEAMRMIDQGGVRLNGEKVSDKGLVLKAGAVVVLQVGKRK
                     FARVTLR"
     gene            1322..2644
                     /locus_tag="PLH85_11130"
     CDS             1322..2644
                     /locus_tag="PLH85_11130"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB65163.1"
                     /translation="MLREKTGILAHLKLLRDGDRKALLKSMALGAGVFTGMAAALAVA
                     PGQDAPPPAETIVTQLERPAFDISGRDEHPFIREDRVLAGETLGALLKRLGVSDTSLT
                     AHAGSPALMRDLGTAFRPGMVVTVATTTDGRLQSASFLRPGNDDQVVLTAENGQLVVR
                     KQALELDRRIHMQSGTIQSSLFAATDAAGLPDSIAEELARMFGSEIDFHSDLRKGDRF
                     SVIYEVFYHHGQAIRTGRILAAEFFNQGVKHSAYLFRHADGKTEYYGPDGRSLRAGFL
                     RSPLEFSRVTSGFSMRLHPILGTWREHKGVDYGAPTGTAVHATADGIVDFAGWQNGYG
                     NFVVLRHQDRYSTAYGHLSAVARGLRKGDRVSQNTVIGYVGSTGWATGPHLHYEFRIN
                     EIHQDPLKVSLPAAQPLDRQKLGTFLHDVAQLNAQLTAARQITTARFD"
     gene            complement(2712..3062)
                     /gene="erpA"
                     /locus_tag="PLH85_11135"
     CDS             complement(2712..3062)
                     /gene="erpA"
                     /locus_tag="PLH85_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNB65164.1"
                     /translation="MNAVTEMPAPLVFTDSAVGKVKDLIAEEGNPELKLRVFVTGGGC
                     SGFQYGFTFDEIRNDDDTVMEKDGVMLLIDPMSYQYLVGAEIDYTEGLEGSQFVIRNP
                     NATSTCGCGSSFSA"
     gene            complement(3162..4190)
                     /gene="argC"
                     /locus_tag="PLH85_11140"
     CDS             complement(3162..4190)
                     /gene="argC"
                     /locus_tag="PLH85_11140"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNB65165.1"
                     /translation="MIKVGVVGGTGYTGVELLRLLAQHPEAEIRAITSRGEAGMPVAD
                     LFPSLRKRVDLCFVDPQAADLASCDVVFFATPNGIAMQQAAALVGAGVRVIDLAADFR
                     IKDVAEWEKWYGMKHACPELVAEAVYGLPEVNREAVRQARVVANPGCYPTAVQLGFLP
                     LVEAGVIELDHLVADAKSGVSGAGRKAETHILFSEAADNFQAYGVAGHRHLPEIRQGL
                     AGFAGQAVGLTFVPHLTPMIRGIHATLYGKLKKDVDLQALFEERFAGEPFVDVLPKGS
                     HPSTRSVRSANMCRIAVHRPQGGDTVVVLSVIDNLVKGAAGQAVQNMNILFGLPETTG
                     LGQVPVMP"
     gene            complement(4322..4717)
                     /gene="rpsI"
                     /locus_tag="PLH85_11145"
     CDS             complement(4322..4717)
                     /gene="rpsI"
                     /locus_tag="PLH85_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNB65166.1"
                     /translation="MMAVTYNYGTGRRKSAVARVFIKKGTGNIVVNGKPVDQFFSRET
                     GRMIVRQPLVLTNFADSFDILVNVTGGGESGQAGAVRHGITRALIDYDAELKSPLKKA
                     GLVTRDAREVERKKVGFRKARRRKQFSKR"
     gene            complement(4727..5155)
                     /gene="rplM"
                     /locus_tag="PLH85_11150"
     CDS             complement(4727..5155)
                     /gene="rplM"
                     /locus_tag="PLH85_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNB65167.1"
                     /translation="MKTFSAKPHEVKRDWFVVDATDKVLGRLASEVARRLRGKHKPEY
                     TPHVDTGDYIVVVNVDKLRVTGNKALDKKYYRHSGYPGGIYETNFTKLQQRFPARVLE
                     KAVKGMLPKGPLGYAMLKKMKCYAGGEHPHAAQQPKVLEI"
     gene            5661..6077
                     /locus_tag="PLH85_11155"
     CDS             5661..6077
                     /locus_tag="PLH85_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB65168.1"
                     /translation="MECVIKWVDGMSFLAETGSGHTVMMDGAPEAGGRNIAPRPMELM
                     LAGAGGCTAFDVVLILKRSRQDVTDCKVSLKADRAETDPKVFTRIAFHYTVTGRNLKP
                     DVVERAIKLSYEKYCSATIMLGKTAEIEHTWDIVEA"
     gene            complement(6132..6752)
                     /gene="coq7"
                     /locus_tag="PLH85_11160"
     CDS             complement(6132..6752)
                     /gene="coq7"
                     /locus_tag="PLH85_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNB65169.1"
                     /translation="MIDQLILGFDRALQTVFASASTVRPTPGEDLPETELSDAERRHA
                     AALMRINHCGEICAQALYQGQALMSDNDGIRRALESAAQEETEHLAWTERRIAELGGR
                     KSLLNPLWYGGSLAIGMAAAKIGDGFNLGFLAETERQVEAHLKSHLDDLPQKDGRTRA
                     IIEQMKTDEVAHAETAVRLGAVPLPPPVRGAMKLMSRVMTRTAYWL"
     gene            complement(6749..7342)
                     /locus_tag="PLH85_11165"
     CDS             complement(6749..7342)
                     /locus_tag="PLH85_11165"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HNB65170.1"
                     /translation="MNHGVHVAGARLAGRSGRLASLFFGLCAGLLASVGAVAQVAQQS
                     LFTSPEPPAWAESPVEIPKELPDDAALAPFSVSRAATATFLIDTSSVTRGEDGIWRFA
                     LVIRTAGGAENITYEGIRCATAERRLYAIGRRGEGWTASRNDAWGRIAESSLNRYHAA
                     LFKDYFCPPGLAQPSPAEAVSRLRSGMPILPTGNLTP"
     gene            complement(7366..7959)
                     /locus_tag="PLH85_11170"
     CDS             complement(7366..7959)
                     /locus_tag="PLH85_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB65171.1"
                     /translation="MERMHYQPISQFKVSRGTCAVAEAWGLTRVTASSPAVEVMTDLR
                     RIPAATIGQDVSLAEANHAMILRGVRLLFVTDAARRVVGIVTATDLLGERPTQVARER
                     SLRYEELTLNEIMVPSEALDAVSMSDVLRAEVGHVVATLKSSGRQHALVVEETPEGQP
                     TIRGIFSASQIARQMGIPLQTTEVARTFAEIEAAIAH"
     gene            complement(8133..8693)
                     /locus_tag="PLH85_11175"
     CDS             complement(8133..8693)
                     /locus_tag="PLH85_11175"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNB65172.1"
                     /translation="MLDREGYRPNVGIILVNARNEVFWGKRIREHSWQFPQGGIKHGE
                     SPEQAMYRELYEEVGLKPEHVRIIGRTRGWLRYDVPKHWIKREWRNTYRGQKQIWYLL
                     RLVGRDSDVSLRASNHPEFDAWRWSSYWVPLEAVIEFKRGVYQSALLELARLLFRDRF
                     FNIPDSYLPHPAEPLEGEGADAGPRR"
     gene            8848..10566
                     /locus_tag="PLH85_11180"
     CDS             8848..10566
                     /locus_tag="PLH85_11180"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNB65173.1"
                     /translation="MRASRFFLSTLKEAPSDAEIVSHKLMLRAGLIKRLAGGIYTWMP
                     LGLRALRKVEAIVREEMNRAGAIEVLMPAVQPAELWEESGRWDFYGPELLRFKDRHDR
                     AFVIGPTHEEVITDVARRELRSYRQLPKHFYQIQMKFRDEIRPRFGVMRGREFVMKDG
                     YSFHTSFEDLQREYRNMYDTYHRIFARLGLKFRAVAADTGSIGGTGSHEFHVIAETGE
                     DDIAYCPDSDYAANVELAEALAPATPRPVPAAPLTKVSTPGVKSIEDVAAFLKVPVDT
                     TVKAVVVQGDDSVGGRPVLLLLRGDHELNEVKAQKLAGVRNPLTFATPESIREAFGAA
                     PGSLGPVGFGGRVVMDRTVAAMADFVTGANDDDHHFTGVNIGRDFPEAEIADLRNVVA
                     GDPSPDGLGTLEICRGIEVGHIFQLRTKYSEALGCKFLNEQGKEQVMEMGCYGIGVSR
                     ILGAAIEQNNDARGIIWPDAIAPFQVAIVPMGYAKSDAVRAAADTLYADLSAAGIDVF
                     LDDRDERPGSLLADNELIGTPHRVVIGDRGLKDGVVEYQGRRDAEATRWPVAEASANL
                     LARLKA"
     gene            10569..11198
                     /locus_tag="PLH85_11185"
     CDS             10569..11198
                     /locus_tag="PLH85_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNB65174.1"
                     /translation="MRRLSPRLLRCAAALALAGGCFGAQAGNQIYEPLAASVRAALHA
                     AVSDQAPPDTRFTRSPERARWLDEMSRRLARRMPDEAGRLEFLRAVHYEATRAGLDPQ
                     LVLGLIQVESGFKKYALSTAGARGYMQVMPFWVDLIGTSDSNLFHMRTNLRFGCTILR
                     HYLDIERGDLFRALGRYNGSLGRAEYPNLVRGAWENNWSYALFRVAQNP"
     gene            complement(11289..12401)
                     /gene="mnmA"
                     /locus_tag="PLH85_11190"
     CDS             complement(11289..12401)
                     /gene="mnmA"
                     /locus_tag="PLH85_11190"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNB65175.1"
                     /translation="MSRKEKVIVGMSGGVDSSVAALLLKQQGYDVVGLFMKNWEDDDD
                     DEYCSTRQDLVDVASVADVIGIDLEVVNFSKEYKDRVFADFLREYEAGRTPNPDILCN
                     SEIKFRAFLDHALALGADRIATGHYAQVREWTNDGRTEFQLLKAEDGTKDQSYFLYRL
                     NQAQLSKTLFPIGHLYKREVRRMAEAAGLHVFDKKDSTGICFIGERPFREFLSRYLPA
                     RPGEIRTLEGDKVIGQHQGLMYHTLGQRKGLQIGGIKGNQDEAGEHDAWYVARKDVAK
                     NVLYVVQGHDHPALLRDRLAAAELSWVSGRDPRTHWVYTAKPRYRTPDAPCEIDALAD
                     GRAEIAFAQPQWAVTPGQSVVIYESRVCLGGGIIVE"
     gene            complement(12391..12942)
                     /locus_tag="PLH85_11195"
     CDS             complement(12391..12942)
                     /locus_tag="PLH85_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65176.1"
                     /translation="MRALGWLVLLALAGSTWADESVSICYDYGCAAEARVVYSEPRLV
                     EIRARLALADNAVAERAVLAQVVGQLYRWAGEQSPIHADRAGDFLDDGVDGKMDCIDH
                     AESTTRLLHMLEARGMLRFHDVDQIRRRTRWLVTQHFSAVIRARDEGAARFVVDSWFG
                     EHGDPAVILPLEEWLDGEGPNVS"
     gene            complement(12942..13394)
                     /locus_tag="PLH85_11200"
     CDS             complement(12942..13394)
                     /locus_tag="PLH85_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB65177.1"
                     /translation="MDIWKPNVTVAAVVERDGRFLLVEEETDAGVRFNQPAGHLDQGE
                     SLVTAAAREALEETAHHFVPEFLVGIYQWPRPAGDITYLRFAFGGQVTGFEPERALDS
                     GILRAVWMTLDEVRATRERHRSPLILQCCEDYRAGRRYPLDLIRHYDA"
     gene            13521..14441
                     /locus_tag="PLH85_11205"
     CDS             13521..14441
                     /locus_tag="PLH85_11205"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNB65178.1"
                     /translation="MSMADRDGFIWYDGKLVPWREATTHVLTHSLHYGLSVFEGVRAY
                     NTVKGTAIFRLAEHTQRLLNSGKIYMMNIPYSKEELMEAQLEVVRVNKLESCYLRPIA
                     FYGSEKMGISTKGATVHVAIAAWPWGAYLGEDGLEKGIRVKTSSFARHHVNVTMPRAK
                     LASTYANSILANLEATEAGYDEALLLDTNGFVAEGAGENLFVIKDGAIYEPEIASALT
                     GITRASVIQVAKEFGLDVISKRITRDDIYIADEAFFTGTAAEVTPIRELDGRVIGEGK
                     RGPVTQKLQARFFDIVNGRAPEYAHWLSFI"
     gene            14461..14661
                     /locus_tag="PLH85_11210"
     CDS             14461..14661
                     /locus_tag="PLH85_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNB65179.1"
                     /translation="MPENQDKAQAIEVTAKNLPLHCPMPDAPLWARHPRVFLDVAGEG
                     HATCPYCGAKYVFKGELPKGHH"
     gene            14753..15184
                     /locus_tag="PLH85_11215"
     CDS             14753..15184
                     /locus_tag="PLH85_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB65180.1"
                     /translation="MSSILYTSPDDAEAAFYDAMGRADLNAMMTIWSEDEEVVCIHPG
                     SIRLVGLGAIRESWRQLFASGMRLHVRPTHLIRWHNLMTAVHTVHQRVHVEGDDRLHP
                     PIIATNVYTRGASGWRMVMHHTSSSPDVDNLQGHDGPHVVH"
     gene            15181..16182
                     /locus_tag="PLH85_11220"
     CDS             15181..16182
                     /locus_tag="PLH85_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNB65181.1"
                     /translation="MTIPPYRAPRWLPGGHAQTIWPLRCTGPLPNFQRERWTTPDGDF
                     IDLDWLPLQPAAPLVVLFHGLEGSSHSPYARALMGHLASLGWNGVVPHFRGCSGEPNR
                     APRAYHSGDADEIEWILTRMAERFPDRPRYAAGVSLGGNALLLWLGTRGTEARRTIDA
                     AAAICAPLDLAAAGHHLARGFNRVYTRHFLSTLKRSAEAKLDRFPGLFDRAAMLRAET
                     LHAFDNVVTAPLHGFRDTDDYWRRASSKPHLRHIAVPTLVLNARNDPFLPAHHLPDVS
                     QVSSAVHLEHPEEGGHAGFVRGPWPGRLDWLPARLVHFFTNLNGSFHPLKSVMLHCV"
     gene            16218..17600
                     /locus_tag="PLH85_11225"
     CDS             16218..17600
                     /locus_tag="PLH85_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNB65182.1"
                     /translation="MFVPAPEIFKAYDIRGIVDKTLTAEAVRAIGHALGSEAVARGQK
                     AIAVGRDGRLSGPELAGALADGIRAAGVDVVDVGCVPTPLTYFAAYELGTHSCVSVTG
                     SHNPPDYNGLKMVLGGQTLFGDLIQSLRQRIIDGNLVTAAEPGRLTHADVVPAYIAKI
                     VGDVKLSRPMKIVMDCGNGVAGAVAPELFKRLGCELVELFCEVDGHFPNHHPDPSKPE
                     NLEDLIRALRDTDAEIGLAFDGDGDRLGVVTKDGEIIFPDRQLMLFAEDVLTRVPGGE
                     IIYDVKCTRNLAGWIQARGGKPTMWNTGHALVKAKLKETGAPLAGEMSGHVFFKERWY
                     GFDDGLYTGARLLEIVSRHADANPVLKGLPNATSTPELNLKMAEGEPFALVDKLKAEA
                     RFEGAESILTIDGVRVEYADGFGLARPSNTTPVVVLRFEADNEAAIARIQADFRRAIE
                     GVWPGVQLPF"
     gene            complement(17691..18242)
                     /locus_tag="PLH85_11230"
     CDS             complement(17691..18242)
                     /locus_tag="PLH85_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB65183.1"
                     /translation="MTPQNWRFCPQCGAPLEWGPVAGAERQACYAPGCGFVRWDNPVP
                     VVAAVIECVDRDGAVLLARNHVWPERMFALVTGFLERGESPEEAVAREVREETALEAE
                     SVRLLGVYPFERKNEVIIGFHVRARGEIVLNEELAEFRLVPPARLRPWPRATGLFLRD
                     WLLVQGLPVPELVEDNLFAHADL"
     gene            complement(18247..>20726)
                     /gene="alaS"
                     /locus_tag="PLH85_11235"
     CDS             complement(18247..>20726)
                     /gene="alaS"
                     /locus_tag="PLH85_11235"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNB65184.1"
                     /translation="LGFDKRPYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLG
                     NFSFGDYFKRDAIHYAWELLTEVFKLPKDKLTVTVYAEDDEAYDIWTKEVGVPADKVI
                     RIGDNKGARYASDNFWMMGDTGPCGPCTEIFYDHGAHIWGGPPGSPEEDGDRFIEIWN
                     NVFMQFNRDEAGVMHPLPKPSVDTGMGLERISAVLQGVHANYEIDLFQTLLKAAARET
                     SAADLDSPSLKVLADHIRACSFLIADGIIPGNEGRGYVLRRIIRRAIRHGYKLGVRAA
                     FFHRLVPDLVAEMGDAYPELKSHQQKVTDVLKQEEERFFATIEHGMAILEADLAEMSK
                     AGSTVFNGDTAFKLHDTYGFPLDLTADVCREHGVTVDNAAFDAAMARQKEQARAAGKF
                     KMAANLEYDGPATTFHGYDSLEHGGNILALYKDGAAVNELHEGDLGVVVLDDTPFYAE
                     SGGQVGDRGELRSVHGIFAVEDTQKIQASVFGHHGVVKTGTLSVGNGVAARVDLQART
                     RTMRNHSATHLMHKALRQVLGEHVQQKGSQVDPDKTRFDFVHTQPMSNEQIRQVENIV
                     NAEILANTPCETRVLPIEEAQKLGAMMLFGEKYGDSVRVLDIGFSRELCGGTHVQRTG
                     DIGLFSIVAEGGVAAGVRRVEAVTGDNALRYVQGMETALGGVAGTLKVLRSEVPARVN
                     ALLDQTRRLERELAALKGKLASAQGDDLGSRAVEVKGVKVLAASLDGADSVTLRETLD
                     KLKDKLKSAIIVLATVADGKVSLIAGVTADLTGKVKAGELVNFVAQQVGGKGGGRPDM
                     AQAGGTDAAALPAALASVTDWVGAKLG"
BASE COUNT         3767 a         7227 c         6175 g         3557 t
ORIGIN      
        1 gcattataaa gaactattca acgacgtcaa caacattgga aaaataaatc agcgcagcgt
       61 gacgcgggca aattttcgct tgcccacttg aagaacgacc acagcccccg ccttcagcac
      121 caagcctttg tccgacacct tttcaccatt cagccgaacg cccccctgat cgatcatgcg
      181 catcgcctcc gaggtgctgc cagtgagtcc cgcctgcttg agcacggcga ccacgctgag
      241 gccggtttcg cccccggaga tcgccacttc cggcagatcg tccgggatcg cattgcgctg
      301 aaagcgcgcc tcgaagtccg tgagcgccgc ttcagccgcc ggctcaccat ggaagcgcgc
      361 gacgatctcc tgggccaggg ccactttgac atccctcgga ttgcgcccgg tctgtaccgc
      421 ctccttcaac ccggccacct cggccgatga gcggaacgat agcaggtcgt aatagcgcca
      481 catgagatcg tccgagacgg acatgatctt gccgaagatc tcccccgggg gctcatagat
      541 tccgatgtag ttgcccagag actttgacat cttgttgacg ccgtccagcc cctccaagag
      601 cggcatggtc agcacgcact ggggcgattg accggcattc ttctgcaact cccgccccat
      661 cagcagatta aagcgctgat cggtcccgcc cagttccaca tccgccttca atgcgaccga
      721 gtcatagccc tggcacaagg ggtacaggaa ctcatgaatg gcgatgggtt gctggttgcc
      781 aaaacgcttg gagaaatcgt cccgctccag catgcgggcc accgtgtgct gcgccgccag
      841 acggatcatg ccggacgcgc cgagggattc catccacgtg gagttgaaga ccacctcggt
      901 gcgcgccgga tcgagaatct tgaacacctg ctcctgatag gtctgggcat tggcaagcac
      961 ctgctcccgg gacaggggcg ggcgtgtcgc attcttgccc gacggatcac cgatcatccc
     1021 cgtgaagtcg ccaatcagga acagcacctg atgccccaga tcctggaagt ggcgcagctt
     1081 gttgatgagc actgtatgtc ccagatgcag atcgggcgcc gtcggatcga agccggcctt
     1141 gacccgcagc gggcggcccg acttgagctt gtccaccagc tcggattcga ccagaagctc
     1201 gtccgagccg cgcttgatga gttcgagtgc ttgcgtgatt tccgtcatat ctgttccaga
     1261 ccaaggcagg cccgcgcgct ttttggtaga ctccgcggct cgattttgct tggaaagtcc
     1321 ggtgcttaga gaaaagacgg ggattctagc gcatctcaaa ttgttgcgtg acggcgatcg
     1381 gaaggcgctg ctgaagtcga tggcgctcgg cgccggagta ttcacgggca tggcagccgc
     1441 gctcgccgtg gctcccggtc aggacgctcc gccgccggca gagacgatcg tcacacagct
     1501 ggaacgcccg gccttcgaca tttcgggccg tgacgagcac cctttcatcc gtgaagacag
     1561 agtcctcgcc ggagagacgc tcggggctct gctcaagcga ctcggcgtct cggacacgtc
     1621 cctgaccgcc cacgccggca gtcctgccct gatgcgcgat ctgggaacag ctttccggcc
     1681 aggcatggtg gtcacggtcg ccacaaccac ggacggccgc ctgcaatccg cctctttcct
     1741 ccgcccgggt aatgatgatc aggtcgtgct gaccgcggaa aacggccagc tcgtcgtccg
     1801 caagcaggcc ctcgagctgg accgacgcat ccacatgcag tcgggcacca tccagagctc
     1861 cctctttgcc gccaccgacg cggccggcct gcccgacagc atcgccgaag aactcgcccg
     1921 gatgttcggc agcgagatcg acttccactc cgacctgcgc aagggtgacc gtttcagcgt
     1981 catctacgag gtcttttacc accacggaca ggcgatccgc actggccgca tcctcgccgc
     2041 cgaattcttc aaccagggcg tgaaacactc ggcctacctg ttccgccacg cggacggcaa
     2101 gaccgaatac tacggaccgg acggacgcag cctgcgcgcg ggctttttgc gctcgccact
     2161 ggaattctca cgggtcactt ccggattttc gatgcgcctg catccgattc tgggtacttg
     2221 gcgcgagcat aagggcgtcg actacggcgc tccaacgggc acggccgtgc atgcaaccgc
     2281 cgatggcatc gtggactttg ccggctggca aaacggttac ggaaatttcg tggtgctccg
     2341 ccaccaggac cgctactcca cggcctacgg ccacctgagc gctgtcgcgc gtggattgcg
     2401 caagggggac cgcgtcagcc agaacaccgt gatcggctac gtgggctcga ccggctgggc
     2461 cacgggacca cacctgcact atgagttccg aatcaacgaa atccatcagg acccgcttaa
     2521 ggtcagcctg cctgccgccc agcctcttga tcgccagaag ctgggcacat tcctccatga
     2581 cgttgctcaa ctcaacgccc aactgactgc agcccgccag atcaccacgg cccgcttcga
     2641 ctgatccaac ggtcacaggc acaaaaaagg gcgccaacag gcgccctttt catgcaatgc
     2701 gtacggggag gtcaggccga gaaggacgac ccacagccac aggtgctggt cgcattcgga
     2761 ttgcggatca cgaactggga gccttccaga ccttccgtgt agtcgatctc cgcacccacg
     2821 aggtattggt aactcatcgg gtcgatcaac agcatcacgc cgtccttttc catcacggtg
     2881 tcatcgtcgt tacggatctc gtcgaacgtg aacccgtact ggaacccgga gcaaccgccg
     2941 cccgtcacga aaacgcgaag cttcagctcg ggattacctt cctcggcgat cagatccttg
     3001 accttgccga cggcactatc agtgaacacc agcggtgccg gcatctcggt tacagcattc
     3061 atgggggaac tccttcaggt cagtcttcaa attatgggta gccgccagaa gccggtcaag
     3121 ccacacgagt ggctagttga ccgggaaacg ggcgataagt tctacggcat caccggcacc
     3181 tgccccaacc cggtcgtttc cggcaggcca aagagaatgt tcatattctg aacggcctgc
     3241 cccgcagccc ccttgacgag gttgtcgatg accgacagca ccacgacggt atcgcccccc
     3301 tgaggtcgat gcaccgcgat tcgacacatg tttgcagaac ggaccgaacg ggtcgaggga
     3361 tgggaaccct tgggcagcac atcgacgaag ggctctcccg caaagcgctc ctcgaacaac
     3421 gcctgcagat ccacgtcttt tttgagtttg ccgtagagag tggcatgaat accccgaatc
     3481 atcggggtca ggtgcggcac gaaggtcagt cccacggcct gccctgcaaa cccggccaat
     3541 ccctgccgga tctcaggcag atggcgatgt cccgctacgc cgtacgcctg aaaattgtct
     3601 gccgcctcgg aaaacaggat atgcgtttcg gccttccgac ccgcgcctga cacaccggac
     3661 ttggcgtcgg cgaccagatg atccagctcg atcaccccgg cctccacaag cggtagaaaa
     3721 cccagttgta ccgcagtggg gtagcagccg ggattggcga ccacccgtgc ctggcgtacc
     3781 gcttcgcgat tcacttcagg caggccgtac accgcttcag cgaccagttc cggacaggcg
     3841 tgcttcatcc cgtaccactt ctcccattcg gccacatcct taatccggaa atccgccgcc
     3901 agatcgatca cacgcacgcc cgcaccgacc agcgcggcgg cctgttgcat ggcaatgccg
     3961 ttgggcgtgg cgaagaacac cacatcgcaa gatgccagat cagccgcctg gggatccacg
     4021 aagcacaggt cgacacgctt gcgcagactg ggaaacagat ccgccaccgg catgcccgcc
     4081 tcaccgcggg acgtaatcgc acggatctct gcctccggat gctgggccag cagccgcagc
     4141 agttccacgc cggtataccc ggtccccccg accacaccca ccttgatcat gctaactctc
     4201 cgaactggaa cacgaaatca gtgccgcatt tgaccacaac ccggacacgg accaccagat
     4261 tacggccgga agatgacaaa aagccgcccg aaggcggcct ttcgacaaaa ccatccagcg
     4321 cttaacgctt ggagaactgc ttgcgacggc gcgccttgcg gaagccaacc ttcttacgct
     4381 cgacttcacg ggcatcgcgg gtcaccagac cggccttctt cagcggggac ttgagttccg
     4441 catcgtagtc gatcagggcg cgggtgatgc cgtgacggac cgcgccagcc tggccggact
     4501 caccaccacc ggtgacattc accaggatgt caaagctgtc ggcaaagttc gtcagcacca
     4561 gcggctggcg tacgatcatg cggccagttt cgcgggagaa aaactgatcc accggcttgc
     4621 cgttcacgac gatgttgccg gtacccttct tgataaagac acgggccacc gccgacttgc
     4681 ggcgaccggt accgtagttg taagtcacag ccatcatcgg ctcctgttag atctcgagaa
     4741 ctttgggctg ctgtgcagcg tggggatgct cgccaccggc gtagcacttc atcttcttca
     4801 gcatggcgta gccgagcggg cccttgggca gcatgccctt gacggccttt tcaagcacgc
     4861 gcgccgggaa gcgctgctgc agcttggtga agttggtttc gtagataccg ccggggtagc
     4921 cggagtgacg gtagtacttc ttgtccagcg ccttgttccc ggtcacacgc agcttgtcca
     4981 cgttgacgac gacgatgtag tcgccggtat ccacgtgggg ggtgtattcg ggcttatgtt
     5041 tgccacgcaa gcgacgcgca acctcggaag ccagccgacc caggactttg tccgtcgcgt
     5101 ccaccacgaa ccagtcgcgc ttgacctcat gcggcttggc agaaaacgtt ttcattgaag
     5161 aattcctcag acttgtcccg ctcgggaccg accacaattc ggaaagccgc agattctatc
     5221 cctgcgaacg cctggatgtc aaacccttgc tcaagataaa aaaaagcgca gatcggggag
     5281 atctgcgcta aatccacacc aaaggaggag ggtggaggag acaccgcttc agtcgggtca
     5341 ggtgaaacaa accgactgga ctgagttcat tatgggtcga ccggacctgt gatgcaagca
     5401 ctttttgtgc aatgcgtcat aaatatttta atcgttcaat aaattttatt tattgcaaaa
     5461 attcactgca actctatgtt tttaattatt ttttttgaat tgaaaacagc catttcggca
     5521 taagcaagca cgtttcaaag gccagcatgc tgcagcgcac aacacctgcc cgcgaaggct
     5581 gggcaggccg ggtacaatgg cgcctctacg gcacgggctc gccgcaccgc gccttcacac
     5641 gacacgcgag gaagcaagca atggaatgcg tcattaagtg ggtggacggt atgagttttt
     5701 tggccgaaac aggttccggc cacacggtca tgatggacgg cgcgccggag gcgggagggc
     5761 gcaacatcgc ccctcgtccg atggaactca tgctggccgg cgcgggaggc tgcacggcct
     5821 tcgacgtcgt gctcatcctc aagcgcagcc gccaggacgt gacggactgc aaggtcagtc
     5881 tcaaggccga ccgggccgag accgacccga aagtgttcac acggatcgct ttccactaca
     5941 ccgtgacggg ccgcaacctc aagccggacg tcgtggagcg cgccatcaag ctctcgtatg
     6001 aaaagtattg ctcggccacc atcatgctcg gcaagaccgc cgagatcgag catacctggg
     6061 acatcgtcga ggcataaacg cggtcagccc cacccgcacc gagccgccag agcggctcgc
     6121 cggacaccga actacagcca gtaggccgtg cgggtcatga cccgcgacat gagcttcatc
     6181 gccccgcgca cgggcggggg cagcggcact gcccccaacc gcacggccgt ctccgcgtgc
     6241 gccacctcgt ccgtcttcat ctgttcgatg atggcccggg tccgtccgtc tttctggggc
     6301 agatcgtcca gatgactttt caggtgcgcc tccacctgcc gttcggtttc ggccaggaat
     6361 cccaggttga aaccgtcgcc gatcttcgcc gcagccatgc cgatggccag cgagcccccg
     6421 taccagagcg gattgagcag actcttgcgc ccacccagtt cggcgatgcg ccgctccgtc
     6481 cacgccaggt gctcggtttc ctcctgggcg gcagactcaa gcgcacgccg gatgccgtcg
     6541 ttgtcagaca tcagcgcctg gccctggtag agggcttggg cgcagatctc accgcaatga
     6601 ttgatccgca tcagcgcggc ggcgtggcgc cgctccgcat cgctcagttc cgtctcggga
     6661 aggtcctcgc ccggggtcgg gcggacggtg gatgcactgg caaacacggt ctgcaaggca
     6721 cggtcaaatc cgagaatgag ctggtcgatc atggcgtcag gtttccggtt ggaaggatgg
     6781 gcattccgct gcgcaggcgg gaaaccgcct cggcaggact gggttgggcc agcccgggcg
     6841 gacagaagta gtccttgaac agggccgcgt gataacggtt gagcgagctt tcggcgatgc
     6901 gcccccatgc gtcgttgcgc gaagcggtcc agccttcgcc acgccgaccg atggcataca
     6961 gtcgacgctc cgccgtcgca caacggatcc cttcatacgt gatgttctcg gcgccgccag
     7021 cagtacggat caccagcgca aatcgccaga tgccgtcctc gccgcgcgtc acgctggagg
     7081 tatcgatcag aaacgtcgcc gtcgccgccc ggctgactga aaacggcgca agcgccgcgt
     7141 catccggcag ctccttggga atctccaccg gcgattccgc ccacgcgggc ggctcgggcg
     7201 acgtgaagag cgactgctgg gcgacctggg cgaccgcccc gaccgaggcc agcagccctg
     7261 cacaaagtcc gaaaaaaagg ctggccagac gtccggaacg tcccgccagc ctggctcccg
     7321 ctacatgcac accgtgattc aaaacatatc ctctggccac gcccctcaat gggcaatcgc
     7381 cgcttcgatt tcagcaaagg tgcgcgccac ttcggtcgtc tgcagcggga tgcccatctg
     7441 ccgggcaatc tgcgatgccg agaagattcc gcggatcgtc ggctgtccct cgggcgtctc
     7501 ttccacgacc agcgcgtgct gacgcccgga cgacttcaac gtcgccacca cgtgtccaac
     7561 ctcggcgcgc agtacgtcgc tcatgctgac cgcatcgagc gcctcggagg gcaccatgat
     7621 ctcgttgagg gtgagctcct catatcgcag ggagcgctcc cgcgccacct gggtgggacg
     7681 ctcgccgagc aggtccgttg ccgtgacgat ccccaccacc cgacgcgccg cgtcggtcac
     7741 gaacaggagc cggacgccgc gcaggatcat ggcgtgattg gcctccgcga gggaaacgtc
     7801 ctgtccgatg gtggcggcgg gaatccgtcg caggtcggtc atcacctcca cggccggcga
     7861 cgacgcggta acccgtgtca ggccccaggc ctcggcgacc gcacaggtcc cgcggctgac
     7921 cttgaactgg ctgatcggct gatagtgcat gcgctccatg gtgtctcctc ccaagcgtgc
     7981 cctgcaaaga ttccagggca tcctgccccg gaacgcccgc gccgcatctc aaagctgacg
     8041 cgacgcccta caaacgcttc gatcagactt gcgcggcacg gacaagtttc ccgcggcgca
     8101 aaatcgaacc ggccgacgcc gcgcgcacgc cgtcaacgac ggggccccgc atcggcccct
     8161 tcaccttcca gcggctctgc cgggtgcggc aggtaactgt cgggaatgtt gaagaaccgg
     8221 tcacggaaaa ggagccgggc cagctcaagc agggccgact ggtaaacgcc gcgcttgaac
     8281 tcgatgaccg cctccagcgg cacccaatac gaactccagc gccaggcatc gaattccggg
     8341 tgattgctcg cacgcaggct cacgtccgag tcgcgcccca ccagccgcag cagataccag
     8401 atctgcttct gaccgcggta ggtattgcgc cactcgcgct tgatccagtg cttgggcacg
     8461 tcgtaccgca gccagccgcg agtgcggccg atgatacgga cgtgctcagg cttcaggccc
     8521 acttcctcgt agagttcccg gtacatcgcc tgttccggcg actcaccgtg cttgatcccg
     8581 ccttgcggaa actgccagga atgctctcgt atccgtttgc cccagaacac ctcgttacgc
     8641 gcattcacca ggatgatgcc gacgttaggg cgatagcctt cacggtcgag catggtgcaa
     8701 ccttcaattc atgaactggt gagaattctt ccatatttcc ccggcccgcg aaagacgaag
     8761 ccccataacg gtgcatcgca acaaaagcgt gccgggacct gggtccacga tagaattgga
     8821 ggttgtttct ttcacggatg ttccccgatg cgcgcctccc gtttcttcct ctcgaccctc
     8881 aaggaagccc cgtccgacgc cgaaatcgtc agtcacaagc tcatgctgcg ggccggcctg
     8941 atcaaacgcc tggcgggcgg catctacacc tggatgcccc tgggcctgcg tgccctgcgc
     9001 aaggtggagg ccatcgtgag ggaagagatg aaccgcgccg gcgccatcga ggtgctgatg
     9061 ccggccgtgc agccggccga actgtgggag gaatccggcc gctgggattt ctatggcccc
     9121 gagctgctgc gcttcaagga ccgccacgac cgcgctttcg tgatcggccc gacccacgaa
     9181 gaagtcatca ccgacgtggc ccgccgcgag ctgcgcagct accgccagct gcccaagcat
     9241 ttctaccaga tccagatgaa gttccgcgac gagatccgcc cccgcttcgg cgtgatgcgc
     9301 ggccgcgagt tcgtgatgaa ggacggctac tccttccaca cctccttcga ggatctgcag
     9361 cgggaatacc gcaacatgta cgacacctac caccgcatct tcgcccgtct gggcctgaag
     9421 ttccgcgcgg tggcggcgga caccggttcc atcggcggca ccggttccca tgagttccac
     9481 gtcatcgccg agaccggcga ggacgacatc gcctactgcc ccgactccga ctacgccgcc
     9541 aacgtggagc tggccgaggc ccttgctccg gccacgccgc gcccggtgcc ggcggcgccc
     9601 ttgaccaagg tgtccacgcc gggcgtcaag agcatcgaag acgtggcggc cttcctgaag
     9661 gtgccggtag acaccaccgt caaggcggta gtcgtgcaag gcgacgacag cgtcggcggc
     9721 cggccggtac tgctgctcct gcgcggcgat cacgaactca acgaggtcaa ggcccagaag
     9781 ctggccggcg tgcgcaatcc cctcaccttc gccacccccg aatccatccg cgaagccttc
     9841 ggcgccgcgc ccggttccct cggcccggtg ggcttcggtg gccgcgtggt gatggaccgc
     9901 acggtggcgg ccatggccga tttcgtcacc ggcgccaacg acgacgatca ccacttcacc
     9961 ggagtgaaca tcggccgcga cttccccgag gccgagatcg ccgacctgcg caacgtggtc
    10021 gccggcgatc cgtcgccgga cggcctgggc accctcgaga tctgccgcgg catcgaagtg
    10081 ggccacatct tccagttgcg caccaagtac tccgaagccc tcggctgcaa gttcctcaac
    10141 gagcagggca aggaacaggt catggagatg ggctgctacg gcatcggcgt atcgcgcatc
    10201 ctcggcgccg ccatcgagca gaacaacgac gcccgcggca tcatctggcc cgacgccatc
    10261 gcccccttcc aggtggccat cgtgcccatg ggctacgcca agtccgacgc cgtgcgggcc
    10321 gccgccgaca ccctctacgc cgacctgagc gccgccggca tcgatgtctt cctggacgac
    10381 cgggacgagc gaccgggttc cctgctggcc gacaacgagc tgatcggcac cccgcaccgg
    10441 gttgtcatcg gcgaccgcgg cctcaaggac ggcgtggtgg aataccaggg ccgccgcgac
    10501 gccgaagcca cccgttggcc ggtggcagaa gcctccgcca atctgctggc ccgcctcaag
    10561 gcttgagcat gcgccgcctg agcccgcgcc tcctccgatg cgccgccgcc ctggcgctgg
    10621 cgggtgggtg cttcggtgcg caggccggca accagatcta cgaacccctc gccgccagcg
    10681 tgcgtgcagc cctgcatgcc gcggtgagcg accaggcgcc gcccgacacc cgcttcaccc
    10741 gctcgccgga gcgggcccgc tggctggacg aaatgtcccg ccgcctcgcc cgccgcatgc
    10801 cggacgaggc cggccgcctc gaattcctgc gggcggtcca ttacgaagcc acccgcgccg
    10861 gcctcgatcc gcaactcgtg ctggggctga tccaggtgga aagcggtttc aagaagtacg
    10921 cgctgtccac ggccggcgcc cgcggctaca tgcaggtcat gcccttctgg gtcgacctga
    10981 tcggcacctc ggacagcaac ctcttccaca tgcgcaccaa cctgcgcttc ggctgcacga
    11041 tcctgcggca ctacctggat atcgaacgag gcgacctgtt ccgcgccctg ggccgctaca
    11101 acggcagcct gggccgcgcg gaatatccca atctggtccg cggtgcgtgg gagaacaact
    11161 ggtcctatgc cctcttccgg gtcgcccaga acccctgacg ccggagcgcc cggccccgct
    11221 gcaagactca atgaaaacgg accgcccgcg cgatccgttt tcagcgtctc aagcgaggtt
    11281 cagggccctc attccacgat gattccgccc cccaggcaca cccggctctc gtagatcact
    11341 acgctctgac cgggtgtcac cgcccattgg ggctgggcaa aggcgatctc ggcgcggccg
    11401 tccgccaatg cgtcgatctc gcagggcgcg tccggcgtgc ggtaacgcgg cttggcggtg
    11461 tacacccaat gggtgcgcgg atcgcgaccg gacacccagg acagctctgc cgccgccagc
    11521 cgatcccgga gcagggccgg atggtcgtgg ccctgcacca catacagcac attcttcgcc
    11581 acatccttgc gtgccacgta ccaggcgtcg tgttcgcccg cctcgtcttg gttgcccttg
    11641 atgccgccaa tctgcagccc cttgcgctgg ccgagggtgt ggtacatcag gccctgatgc
    11701 tgtccgatca ccttgtcacc ctccagcgtg cggatctcgc ccggtctggc cggcagatag
    11761 cgggacagga actcccggaa cggccgctca ccgatgaagc agatgccggt cgaatccttt
    11821 ttgtcaaaga cgtgcaggcc ggcggcctcg gccatgcggc gcacctcgcg cttgtagaga
    11881 tggccgatgg gaaacagcgt cttggaaagc tgcgcctgat tgaggcggta caggaagtaa
    11941 ctctgatcct tggtgccgtc ctcggccttg agcaactgga attcggtacg cccgtcgttg
    12001 gtccattccc gcacctgggc atagtgaccg gtggcaatgc ggtcggcgcc gagggccagg
    12061 gcgtggtcca ggaaggcccg gaacttgatc tcggagttgc acaggatgtc cggattgggt
    12121 gtgcggcccg cttcgtattc ccgcaggaaa tcggcaaaca cccggtcctt gtattccttt
    12181 gaaaaattca cgacctccag gtcgatgccg atcacgtcgg cgaccgaggc cacatccacc
    12241 agatcctgac gtgtcgaaca gtactcgtcg tcatcgtcgt cctcccagtt cttcatgaac
    12301 aggccgacca cgtcgtagcc ctgctgcttg agcagcaagg ccgccacgga cgaatccaca
    12361 ccgccggaca tgccgacgat caccttttcc ttacgagaca ttcggtcctt ctccatcgag
    12421 ccattcttca aggggcagga tcacggccgg atcgccgtgc tccccgaacc acgaatccac
    12481 cacaaaccgc gcggcaccct cgtcccgcgc acggatcacg gcagaaaaat gctgggtcac
    12541 caaccagcgc gtgcgccggc ggatctggtc cacgtcgtgg aaacgcagca tgccgcgggc
    12601 ttcaagcata tgcagcaggc gggtggtgga ttccgcatga tcgatgcagt ccatcttgcc
    12661 gtccacacca tcatcgagga agtctcccgc acgatccgca tggatcggcg actgctcgcc
    12721 cgcccaacgg taaagctgcc ccaccacctg tgccagcacc gcccgctcgg ccaccgcgtt
    12781 gtcggccagg gcgaggcgtg cgcggatctc gacgaggcga ggctccgaat acaccacccg
    12841 ggcctccgcg gcgcacccat agtcgtaaca gatcgacacc gactcatccg cccaggtgga
    12901 gccggcaagc gccagcaaca ccagccaccc cagggcacgc atcaggcgtc gtagtggcgg
    12961 atcaggtcga gcggatatcg ccgccccgcg cgatagtcct cacaacactg caggatcaag
    13021 gggctgcgat ggcgctcgcg ggtggcgcgt acctcgtcga gggtcatcca cacggcccgc
    13081 aggatccccg aatcgagggc ccgctccggc tcgaagccgg tcacctggcc accaaaggca
    13141 aagcgcaggt aggtgatgtc acccgccggc cgcggccact gatagatgcc caccagaaac
    13201 tcgggtacga aatggtgggc ggtttcctcg agggcctcgc gtgcggcggc cgtcaccagg
    13261 gactccccct ggtcaagatg cccggccggc tggttgaagc gcaccccggc gtcggtttcc
    13321 tcctccacca gcaggaagcg accgtcccgt tccaccacgg cggccaccgt gacattgggt
    13381 ttccagatat ccatcggctt catcgcgatc cgtacggaaa aatgcacatt ctaccccgcg
    13441 ccgatgagcg gaccgcaggc cggcggaatg cgctagaatc gaaggctcga attcaatgca
    13501 tttcgctgga gatccccata atgtcaatgg cagaccgcga cggtttcatc tggtacgacg
    13561 gcaagctcgt gccgtggcgt gaagcgacca cccacgtgct cacccactcc ctccactacg
    13621 gtctttccgt cttcgagggc gtccgcgcgt acaacaccgt caagggcacc gccatcttcc
    13681 gcctcgccga gcacacccag cgcctgctca actccggcaa gatctacatg atgaacattc
    13741 cttacagcaa ggaagaactc atggaagccc agctggaagt cgtgcgcgtc aacaagctgg
    13801 aatcctgcta cctgcgcccc atcgccttct acggctccga gaagatgggc atctccacca
    13861 agggcgccac cgtgcatgtg gccatcgccg cctggccctg gggtgcctac ctgggtgaag
    13921 acggcctcga gaagggcatc cgcgtcaaaa cctcgtcctt cgcgcgtcac cacgtcaatg
    13981 tcaccatgcc ccgcgccaag ctggcctcca cctacgccaa ctccatcctg gccaacctgg
    14041 aagccaccga agccggttac gacgaagccc tgctcctcga caccaacggc ttcgtggccg
    14101 aaggcgctgg cgagaacctc ttcgtgatca aggacggcgc catctacgaa cccgagatcg
    14161 cctccgccct caccggcatc acccgcgcct cggtcatcca ggttgccaag gaattcggcc
    14221 tggacgtcat ctccaagcgc atcacccgcg acgacatcta cattgccgac gaagccttct
    14281 tcaccggcac cgccgccgag gtcacgccga tccgcgaact ggacggccgg gtcatcggcg
    14341 aaggcaagcg gggccccgtc acccagaagc tccaggcccg cttcttcgac atcgtgaacg
    14401 gtcgcgcccc cgaatacgca cactggctgt cattcatctg agatccccgg gagaaccctc
    14461 atgcctgaaa accaggacaa ggcgcaagcc atcgaagtca ccgccaagaa cctgccgctg
    14521 cactgcccga tgccggatgc ccccctgtgg gcccgtcatc cgcgggtctt cctcgacgtc
    14581 gcgggcgaag ggcatgccac ctgcccctac tgtggtgcca agtatgtctt caagggtgaa
    14641 ttgcccaagg gtcatcactg acccgccccg ggccaagcct caccaggcgg gggcctccgg
    14701 gctcccgcag cacgcgtgcg gacctcaccg cagcgccgtc ttgcctatcc ccatgagctc
    14761 catcctgtac acctccccgg acgatgccga ggccgccttc tatgacgcca tgggtcgggc
    14821 cgacctcaac gccatgatga ccatctggtc cgaggacgag gaagtcgtct gcattcaccc
    14881 cggcagcatc cgtctggtgg gcctcggtgc catccgcgaa tcctggcgcc agctcttcgc
    14941 cagcggcatg cgcctccacg tccggccgac ccacctgatc cgctggcaca acctgatgac
    15001 cgcggtgcac accgtgcacc aacgtgtgca tgtggaaggg gacgaccgtc tccacccgcc
    15061 catcattgcc accaacgtct atacccgcgg cgccagtggc tggcgcatgg tcatgcacca
    15121 cacctcgtcc tcccccgacg tggacaatct gcaaggccac gacggccccc acgtggtcca
    15181 ttgaccatcc cgccctaccg cgcgccgcgc tggctgcccg gcgggcatgc ccagacgatc
    15241 tggccgctgc gctgcacggg ccccctcccg aatttccagc gtgagcgctg gaccacgccg
    15301 gacggcgact tcatcgacct ggactggctg cccctccagc ccgccgcccc gctggtcgtg
    15361 ctctttcacg gcctcgaagg ctccagccac tccccctacg cccgcgccct gatgggccac
    15421 ctggccagcc tcggctggaa cggcgtggtc ccccacttcc gcggctgctc cggcgaaccc
    15481 aatcgggcgc cgcgcgccta ccattcgggt gacgcggacg agatcgagtg gatcctgaca
    15541 cgcatggcgg agcgtttccc cgaccggccc cgctacgcgg ccggcgtgtc cctcggtggc
    15601 aatgccctgc tcctgtggct cggcacccgc ggcaccgagg cccgccgcac catcgacgct
    15661 gccgcagcaa tctgcgcgcc gctggatctg gccgccgcgg gtcaccacct cgcccgcggc
    15721 ttcaaccggg tctacacgcg ccacttcctg agcaccctca aacgcagcgc cgaggccaag
    15781 ctcgaccgct tccccggcct cttcgaccgg gccgccatgc tccgggccga aacgctccac
    15841 gcattcgaca acgtcgtcac tgcgccgctg cacggctttc gcgacaccga cgattactgg
    15901 cggcgtgcca gcagcaagcc ccacctgcgc cacatcgcag tgccaaccct ggtcctcaac
    15961 gcccgcaatg acccctttct gccggcccac cacctgcccg acgtttcaca ggtctcgtcg
    16021 gccgtccatc tggaacaccc cgaggaaggc ggtcatgcgg gtttcgtgcg cggcccctgg
    16081 cccggacgtc tggactggct gcccgcccgc ctcgtgcatt ttttcacgaa cctgaatgga
    16141 tcctttcatc cgctgaaaag cgtcatgctg cactgcgtat aatgcggttt tcatctttta
    16201 acactggagt ttcgagcatg ttcgtccccg cccccgagat cttcaaggcc tatgacatcc
    16261 gcggcatcgt ggacaagacc ctgaccgccg aagccgtccg cgccatcggc cacgccctcg
    16321 ggtccgaagc cgtcgcccgc ggccagaagg ccatcgccgt ggggcgcgac ggccgcctct
    16381 ccggccccga gctggccggc gccctcgccg acggcatccg cgccgccggc gtggatgtgg
    16441 tggacgtcgg ctgcgtgccc acgcccctca cctactttgc cgcctacgaa ctgggcaccc
    16501 actcctgcgt gtccgtcacc ggcagccaca atccgccgga ctacaatggc ctcaagatgg
    16561 tcctcggcgg tcagaccctg ttcggggacc tgatccagag cctgcgccag cgcatcatcg
    16621 acggcaacct ggtcaccgcc gccgaacccg gccgcctgac ccacgccgac gtggtgcccg
    16681 cctacatcgc caagatcgtc ggcgacgtca agctgtcgcg cccgatgaag atcgtcatgg
    16741 actgtggtaa cggtgtggcc ggtgcggtgg caccggaact cttcaagcgt ctgggctgcg
    16801 aactcgtaga gctcttctgc gaggtggacg gccacttccc caaccatcac ccggacccct
    16861 ccaagcccga gaacctggag gacctgatcc gcgccctgcg cgacaccgac gccgagatcg
    16921 gcctcgcctt cgacggcgac ggcgaccgac tgggcgtggt caccaaggac ggcgagatca
    16981 tcttccccga tcgccagctc atgctcttcg ccgaagacgt gctcacccgc gtgcccggtg
    17041 gcgagatcat ttacgacgtc aaatgcaccc gcaatctggc cggctggatc caggcccgcg
    17101 gcggcaagcc caccatgtgg aacaccggcc acgccctggt caaggccaag ctcaaggaaa
    17161 ccggtgcccc gctggccggc gagatgagcg gccacgtatt cttcaaggaa cgctggtacg
    17221 gcttcgacga cggcctctac accggcgccc gcctgctgga aatcgtttcc cgccacgccg
    17281 acgccaaccc ggtgctgaag gggctgccca acgccaccag caccccggaa ctcaacctca
    17341 agatggccga aggcgagccc ttcgcgctgg tggacaagct caaggccgaa gcccgttttg
    17401 aaggcgccga gagcatcctc accatcgacg gcgtgcgcgt ggaatacgcc gacggtttcg
    17461 gcctggcccg tccgtccaac accaccccgg tggtcgtgct gcgcttcgag gccgacaacg
    17521 aggccgccat cgcgcgcatc caggccgatt tccgccgcgc catcgaaggc gtgtggccgg
    17581 gagtacagtt gccgttctaa gcatcgccca tgccggtccg cgaccggcag cagtcaaaag
    17641 gccaccccgc ggggtggcct ttgttgttgg cggtgccggc cggaggcacg ctacagatcg
    17701 gcgtgggcga acaggttgtc ctccaccagt tccggcacag gcaggccctg caccagcagc
    17761 cagtcgcgga ggaacaggcc ggttgcccgc ggccacgggc gcaggcgcgc cggcggcacc
    17821 aggcggaatt ccgccagctc ttcgttgagg acgatctcgc cccgtgcccg cacgtgaaaa
    17881 ccgatgatta cctcgttctt gcgctcgaag ggatagacgc ccagcagacg cacggactcg
    17941 gcctccagcg ccgtttcctc ccgcacctcc cgcgccaccg cctcctcggg actttcaccc
    18001 cgctccagaa agccggtcac cagcgcgaac atgcgctcgg gccacacgtg attgcgcgcc
    18061 agcaggacgg caccatcccg atccacgcac tcgatcaccg cggccaccac cggcaccgga
    18121 ttgtcccagc ggacgaagcc acagcccggc gcgtagcagg cctggcgctc ggcccctgcg
    18181 acgggccccc attcgagggg cgcgccgcac tgggggcaga agcgccagtt ctgtggcgtc
    18241 atggattcag cccagcttgg cgccgaccca atcggtcacg gacgccagcg ccgccggcag
    18301 cgctgctgcg tccgttccgc ccgcctgagc catgtccgga cgcccgccac ccttgccgcc
    18361 gacctgctgg gcgacgaagt tgaccagctc gccggccttg accttcccgg tcaggtcggc
    18421 cgtgacgccg gcgatcaggc tgactttccc gtcggccacc gtggccagca cgatgatcgc
    18481 ggacttcagc ttgtccttga gcttgtcgag ggtctcccgc agcgtgaccg aatcggcacc
    18541 gtccagggac gccgccagca ccttcacgcc cttcacctcc acggcgcggc tgcccagatc
    18601 gtcgccctgg gccgacgcca gcttgccctt gagggcggcc agttcgcgct ccagacggcg
    18661 ggtctggtcg agcagggcat tgacccgcgc cggcacctcg ctgcgcagta ccttgagcgt
    18721 cccggccacc ccgcccagcg cggtttccat gccctgcaca tagcgcagcg cgttgtcacc
    18781 ggtcaccgcc tcgacgcggc gcacacctgc cgccacgccc ccttcggcaa cgatgctgaa
    18841 caagccgatg tcgccggtgc gctgtacgtg ggtgccgccg cacagttcgc gggagaagcc
    18901 gatgtcgagc acgcgcacgg aatccccgta cttctcgccg aacagcatca tggcgcccag
    18961 cttctgggcc tcttcgatgg gcagcacgcg ggtctcacag ggcgtattgg ccaggatctc
    19021 agcattgacg atgttctcga cctggcggat ctgctcgttg ctcatgggct gggtgtggac
    19081 gaagtcgaag cgcgtcttgt ccggatccac ctgggagccc ttctgctgca catgctcccc
    19141 cagcacctgg cgcagggcct tgtgcatcag gtgggtggcg gagtggttgc gcatggtgcg
    19201 ggtgcgggcc tgcagatcga cacgggccgc aacgccgttg ccaacgctga gcgtgccggt
    19261 cttgaccacg ccgtggtggc cgaacacgct ggcctggatc ttctgggtgt cctccacggc
    19321 gaagatgccg tgcaccgacc gcagctcgcc ccggtcgccc acctggccgc cggattcggc
    19381 gtagaacggg gtgtcgtcca gcacgacgac acccaggtcg ccttcgtgga gttcattgac
    19441 cgcggcgccg tccttgtaga gggccaggat gttgccaccg tgctccaggc tgtcgtagcc
    19501 gtggaaggtg gtcgccgggc cgtcgtactc cagattggcg gccatcttga atttgccggc
    19561 ggcgcgcgcc tgctccttct ggcgcgccat ggcggcgtcg aaggcggcgt tgtcgacggt
    19621 cacgccgtgc tcgcggcaca cgtcggccgt gaggtccagc gggaagccgt aggtgtcgtg
    19681 cagcttgaag gcggtgtcgc cgttgaacac ggtgctgccc gccttggaca tctcggcgag
    19741 gtcggcttcg aggatggcca tgccgtgctc gatggtggcg aagaagcgct cttcctcctg
    19801 cttgagcacg tccgtcacct tctgctggtg gctcttcagt tcgggatagg cgtcgcccat
    19861 ctcggccacc agatcgggca ccaggcggtg gaagaaggcc gcgcggacgc ccagcttgta
    19921 gccgtggcgg atggcgcggc ggatgatccg gcgcagcacg taaccgcgcc cttcgttgcc
    19981 gggaatgatg ccgtcggcga tcaggaagga acaggcacgg atgtggtcgg ccagcacctt
    20041 gagggacggg ctgtccagat ccgccgcgga ggtttcgcgg gcggcagcct tcagcagggt
    20101 ctggaacagg tcgatctcgt agttggcgtg cacgccctgc agcacggccg agatgcgctc
    20161 caggcccatg ccggtgtcca cgctgggctt gggcagcggg tgcatcactc cggcctcgtc
    20221 ccggttgaac tgcatgaaca cgttgttcca gatctcgatg aaacggtcgc catcctcctc
    20281 gggcgatccc ggcgggccgc cccagatgtg cgcgccgtgg tcgtagaaga tctcagtgca
    20341 cgggccgcag ggaccggtgt cgcccatcat ccagaagttg tcggaggcgt agcgcgcgcc
    20401 tttgttgtcg ccaatgcgga tgaccttgtc ggccggcacg ccgacttcct tcgtccagat
    20461 gtcgtaggcc tcgtcgtcct cggcatacac ggtgacggtg agcttgtcct tgggcagctt
    20521 gaagacctcg gtgagcagct cccacgcgta atggatcgcg tcacgcttaa agtagtcgcc
    20581 gaagctgaag ttgcccaaca tctcgaagaa ggtgtgatgc cgggcggtat agcccacatt
    20641 ctccaggtcg ttgtgcttgc cgccggcgcg cacgcacttc tgggacgtgg tggcgcgggt
    20701 gtaggggcgc ttgtcgaagc cgagga
//
LOCUS       DAOQMM010000065        20570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_930_length_20570_cov_48.407945, whole genome shotgun sequence.
ACCESSION   DAOQMM010000065 DAOQMM010000000
VERSION     DAOQMM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_20570_cov_48.407945"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..594)
                     /locus_tag="PLH85_11240"
     CDS             complement(139..594)
                     /locus_tag="PLH85_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65185.1"
                     /translation="MFTRISVVPAALAAAFGVTVLAGLTGCEHPKPPAPAASIPEPDL
                     KDVAKRFVDPSYAVGALVIGKNGEVLPVSAEGKRIQPCLLPKAPETPTAKAPEIATQG
                     EIQECPKARDTTILDVQSVAVVRHTGSTCVTVGGGVFMGRVMPVYQVCY"
     gene            complement(630..3947)
                     /locus_tag="PLH85_11245"
     CDS             complement(630..3947)
                     /locus_tag="PLH85_11245"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNB65186.1"
                     /translation="MAACAIAFAGAAHAACDQPAGRLAAIEGLVEVRAGVHGPWQPAG
                     LRQMLCPGDQVAVRGPGRAAIALAQDVLVRLDQNTTLTLPGSPTRADTELGLTGGVVH
                     VISRFRKRLGVLTPVVNALVDGTEFVVADGDGRARVSVAEGHVRAVTGAGELSLGAGE
                     ATDVADGAAPGPALVVSPLDAVRWAIHYPQVARPPADALAADPVLRTAVSLAEAGRFR
                     AALDTLGDGGPPAFRAALFLGLGRADEARGVLSAATPEAGVAAVRAVIQVARNDAEAL
                     ATARDAVRSDPASAPARLALSYALQADRQVPAALAAAESATALRPDDPVAWARRAELE
                     LALSRLAAGRESARRALALNPAVPRARALAAFAELLAGRTEAAAEAFDALLADDGSEP
                     LAHLGRALAHLRQGEVAAGRQELEVAVLLDPSNAELRSALGRAYLEEDRDKVAGDQFE
                     LARRLDPASPTPWYFDAFRKLRNRDFLAAVADGEEAVARNESRGVLRSGELLDQDRAA
                     RSASLGEAYREVGFYGPMQARAMDALADDPQSPAAHRLLADAYAGVPRFETARVSELL
                     QAELRQPIGQMPLPPQFVMPALPVLDGPRALSPDESTGLFARKPDHFAFSALGGNQDT
                     GAASLVAAHAWERGQIGVGAFDYRSRALVAGDADTHLSGTRLTGQFALTPASSVFAEL
                     RHTERVSGDQTLSLFDADLKRLRDGVNSDLARVAFRMSRGIGEELLLEASTQQIRDRS
                     SQSAHVDDPLNGSYDVLNGYSARLNARRFSAMYSVQRAESALIAGVTSFKLSGTADQN
                     FDLSIPGFGVFPVFASTFSTRAGRDAAFGYSRWRLNPVVTFLTGAEYTRYDEPTVTKA
                     ERFSGKIGLLLQPRTGTMVRAALFQGVKGSKYDREGLEPTQFNGFNQVFDDFNGTRWT
                     RGALAVDQHFAGGAALGAEVSSRHLDVPGLGCTGEACLAHWHEDLHRVYMALPFGTRV
                     AASAEWRYENSHINDDPRQLSMFPYRLRTDLVPIRLWVKAGTGSINLENWAVRQNARM
                     PDAFTGSDLVGHSAFSVMNLRYSMPLIGKDVVASLGASNVFDRRFRFQNADYNGDPKA
                     PLFYPRRTVLVQLTLRY"
     gene            complement(4122..6104)
                     /locus_tag="PLH85_11250"
     CDS             complement(4122..6104)
                     /locus_tag="PLH85_11250"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB65187.1"
                     /translation="MIRHLARSLRAAVLPLASAAAMGVVLLTSAWDWEEGAGLRLLYA
                     LRGIRPPPDRVMIIALDATSAGALGQSERPERWARSLHAVLVGLLAERGAAVVGFDVL
                     FERSREPAEDDQALAAAFRRAGNVMLVEQLTRETISAPDGTALGSVDRRVRALPLFLD
                     AVAGSGPFVLPKTPEGVFEFWDSLPSAGDQPSLPVLMAERIGAAPSPAARRRVLNLYG
                     PLGSVRTVSYARALALAADPQEGAVAFAGKAVLVGFAESNQSRQADAYRTPFTREDGV
                     DLPGVELLATALGNLLDGSGLTRPEDAATVPPLLAWAALLSLPWLGLGTRRAALVTAI
                     LGGVAVAGSAWAFAVLDLWLPVILAGLVAPAVAALLGIAWHIRAARRRERQLRRAVDL
                     GLTHKGSEALASLLRGREAGRRFHGVCLCSDIEGYTALAENLSPEATRDILNRYFAAF
                     IPVVQAHGGHVMDIVGDAVLSLWLADDAPDEACARARAAVLELHTLMNRAPAEGTLPT
                     RFGLHVGPVFLGELGATHHREIRAVGNIVNTASRIQSANKHLGTRILASAEVTAHGGP
                     GSARPLGRFVLAGQSAPVGLHELRCEALPADADRAFADGLAAWSGGAADWARERFAAT
                     LTLVPGDGPARFYAARCRERGALGADPDGIVVLGSK"
     gene            6134..6778
                     /locus_tag="PLH85_11255"
     CDS             6134..6778
                     /locus_tag="PLH85_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB65188.1"
                     /translation="MNYLDRVDLFAGLDEAQTQSLVDRSRLRSFVASTVVVNEGDEGG
                     SMYVVQSGSLKAYLTDKTGREVTLSLLDEGDYFGELALLDDAPRSASVAAVTRSELLQ
                     VPRSAFLALIEEHPASMQVVVRNLVARIRALSDNVRSLALEDVFRRVTRAFETLAVER
                     DGQRLIERRLTQQDVASLVGASREMINRVLRELVAGGYVEIEQHHIVLRRKLPT"
     gene            6889..7098
                     /locus_tag="PLH85_11260"
     CDS             6889..7098
                     /locus_tag="PLH85_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65189.1"
                     /translation="MCFVDSPISACNAVREMVLTDQTQRECAREHDCPPGRNCPLEGC
                     FAEISGQSEEATRREVAAAVAAHHA"
     gene            7100..7315
                     /locus_tag="PLH85_11265"
     CDS             7100..7315
                     /locus_tag="PLH85_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65190.1"
                     /translation="MANRTLGTRQKQVFRGPAKGPAGRVVPRNPLVLPAAQRKAGPHG
                     KSESAKRQAAERRLNRQLEKLTGEGDG"
     gene            complement(7336..8202)
                     /locus_tag="PLH85_11270"
     CDS             complement(7336..8202)
                     /locus_tag="PLH85_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNB65191.1"
                     /translation="MAPPKLKGLGRGLDALLSGNGDDAQTTEGELQTLPVDALKPGKY
                     QPRTRMDPGSLEELAASIKSQGVMQPILVRPVGSLFDAQRYEIIAGERRWRASRIAGL
                     EAVPCLVREIPDEAALAMSLIENIQREDLNPLEEAAGIQRLVDEFGLTHQQAADAVGR
                     SRPAASNLLRLLQLAQPVQELLMAGDIDMGHARALLPLPGATQVGLANVIAAKGLSVR
                     EAERLAYQALHPKPQSAAPAPDRDILRLEEEVADRLGATVKIKANKKGAGMVTIHFGT
                     LDQLDGLLERLR"
     gene            complement(8253..9023)
                     /locus_tag="PLH85_11275"
     CDS             complement(8253..9023)
                     /locus_tag="PLH85_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNB65192.1"
                     /translation="MARIFCIANQKGGVGKTTTSVNLAAGLAAAGQRVLLIDLDPQGN
                     ATMGSGIDKRSLGRSVYHVLVGLATLAEARVASAAGKYDVLPANRDLAGAEVELVALP
                     RRENRLRDALGSHGDTYDFILIDCPPSLSLLTLNGLCCAHGVIIPMQCEYYALEGLSD
                     LVNSIKKVHANLNRDLKIIGLLRVMFDPRVTLQQQVSAQLEQHFGDKVFRTLVPRNVR
                     LAEAPSHGLPGVVFDRGSKGAQAYLAFAEEMIERIKTL"
     gene            complement(9027..9665)
                     /gene="rsmG"
                     /locus_tag="PLH85_11280"
     CDS             complement(9027..9665)
                     /gene="rsmG"
                     /locus_tag="PLH85_11280"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNB65193.1"
                     /translation="MSMGRALADGIAALGLDLPADTPARLEAFARLLIKWNKVYNLTA
                     LRDEAQVVTHHLLDSLSVLPHLAGVTRLVDVGSGGGLPGVVLAIARPDLQVDSVETVQ
                     KKATFQNQARIELKLEHFRAHHARIESWQPPYGPDAAPDGIISRAFADLADFVNLTAH
                     LAGPDTRLFAMKGVYPADEIARLPAGFVLERCLELAVPGLDAARHLLIVKRT"
     gene            complement(9662..11566)
                     /gene="mnmG"
                     /locus_tag="PLH85_11285"
     CDS             complement(9662..11566)
                     /gene="mnmG"
                     /locus_tag="PLH85_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNB65194.1"
                     /translation="MLYPTRFDVIVVGGGHAGTEAALAAARAGCATLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGIQFRILNASKGPAVRATRAQADRV
                     LYKAAIRRRLENQPNLMLFQQAVDDITVAGDRVTGVVTQIGLRFEAPTVVLTAGTFLN
                     GLIHVGLQNYSAGRAGDPPAIRLGQRLKELALPQGRLKTGTPPRLDARTIDFSVMQEQ
                     PGDDPVPVFSFLGHAGQHPHQLPCWITDTNPRTHDIIRANLDRSPMYSGVIEGVGPRY
                     CPSIEDKIHRFADKDSHHVFLEPEGLDTHEIYPNGISTSLPFDVQLAIVRSIRGLEQC
                     TILRPGYAIEYDYFDPRNLKASLETKSIGGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     LQVKGREAWCPRRDEAYLGVLVDDLITRGVSEPYRMFTSRAEYRLSLREDNADLRLTE
                     TGRQLGLVDDVRWAAFCTKREAIERETARLRATWATPAKVPAEAAEAVLGKAIEREYS
                     FFDLLRRPGVSYAALMGLPGAPEDLATDPQVVEQLEIAAKYQGYIDRQQDEVARQAGA
                     EATRLPADLDYTTVRGLSKEVQLRLNQHRPETIGQASRIQGITPAAISLLLVWLKRQD
                     LARDGERRSA"
     gene            11913..15668
                     /locus_tag="PLH85_11290"
     CDS             11913..15668
                     /locus_tag="PLH85_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65195.1"
                     /translation="MKRGFTTHLVIWAIVLGLGAYLAGTLVHTRGAAQARARNTALTH
                     ARLVAEHAHASIRQVELILDDLAERLPRDALAQGTALPDIRRAALRDLLNTARGRMAG
                     IENVILSDSVGMLTVTAIDPVPMVAIDDRTYFRTVREGAPGQTFLSDAVQGRVSGKWG
                     LMVARRLEDASGRFLGLVAVNLGLDRQFIHFYRSLAPEGDAAIALWSHDWRLLTRYPV
                     VEPEIGKAHLIESLAPLIDADLNETSTVAVSPVDRTVRLIALRRVEDYPLYASFAVAE
                     SDYMAQWRDQARDTMLAILALLVGGGVLSTLEVRSGRTAERVRRLIRETPLGLITLRA
                     GAEGDLLLLQSNPAAHRILQTNLEPHAGERLVSLLPGLTGSPILERVRAVARQGQPWR
                     ASGLPYRDERLQALFDIVAFQVAPGVAGVMFDDVSEREAAVAALNARERDLRRAESLA
                     QLGSWRLRLADRSMTCSDECLRILGARAGMPVSVSTFTALIHPDDRERVRAAWRNLLA
                     GGRMDETYRIGREGRPTTWVRARAEHDPLPGDDITLLGTIQDITQQHEAEQRLKDSED
                     RFHQLFARHASIMLLIEPVSGAIVDANAAAAEFYGYAIETLVGMRIQDINALPPEDVE
                     RERQRAFKEQRNYFVFPHRLADGRLRTVEVYSSPIQAGGQTVLFSIMHDVTEKQLAES
                     RLRLAAGVFDNAHEGIVVTDLSGTILEVNRAYCTLTGYEREDLIGRNPRILQAGPSDE
                     AFYAEMWGHLGRRGFWRGDLHNRRKDGSYYLQETGITAVTNALGLRTHYIGFAEDVTL
                     LRQSQQRLEYLAYHDALTQLPNRLLLADHIRLALAAARREGRHVAVCYLDLDEFKPVN
                     DQWGHTVGDQLLVEVAGRLKACVRAEDTVARLGGDEFVILLASLDSRTEGEQALARVV
                     AALSSPFQLTVGEIGVGASIGVSVFPPDDADADSLIRHADQAMYLAKQAGRNSIRFFD
                     PDADRRARSLRDSVAEVALGLERGEFELYYQPQVHLRSGRVVGVEALLRWNHPQRGLL
                     APGGFLAEVDNTEVAVALGEWVLDTALRQADTWHRAGLELRVGINVSAHHLQQDTFAA
                     RLAERLSRHPELPAGRIELEILETAALEDLSRISRLMETCRRFGVSFALDDFGTGYAS
                     LAYLKHLPANLLKIDQSFIRDMLSDPEDHAIVQGIVGLAMAFRREVIAEGVESLAHGE
                     ALLALGCPLAQGYCIARPMPAAAVAPWVAHWCNPPEWSQPAPPEDRP"
     gene            15665..17662
                     /locus_tag="PLH85_11295"
     CDS             15665..17662
                     /locus_tag="PLH85_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB65196.1"
                     /translation="MKPLCPLPPPVAGLAPPLRACDPWLVVSADARRAALSEAAVRLL
                     ELPDAHAELPATIQTVLGQADGDFVLSLDGADGARFLSVALGPDPDARLGAGTRLAVL
                     HDASGLIRLQRALADSEGRLQAILGAAMDAIIAMDEAGRITLWNAAAERLFGWRADEV
                     RGRDLHETLAGTAERARFLAHAGRWTRGGTSPVFGQILRLPAHRRDGTALDIELSLGA
                     FRRHGHWQALGIVRDVSARVAAELELQVTGTRLQQALEGGNITYWDWDVSTGSLVLGP
                     RYFSMLGYPDDAFPHTAESFRQLVHPDDRPQVEAALKAVLTGQTAEFACDFRLRAADG
                     TDRWATGQGKVTARDAAGRPLRVIGTHRDIHAFHESEAALQRQLAETLRLHRELEEAQ
                     VQLVQSEKMAAIGQLAAGVAHEMNTPLGFVKSNVGTLQTYISPLLEVITALMAATDGL
                     PGGAALADARARAAALDLDFLRQDLPDLFAETRDGIVRVQRIVADLKDFSHVDSATWA
                     HADLHRGLDSTLNILHNDIKHRIQVIRDYGDLPLLWCRPSQLNQVFLNLLANAAQAIA
                     DRGTITVRTRADARHVTVDIADTGCGIAPEHLPRIFDAFFTTKPVGKGTGLGLSISGD
                     IVRKHGGHLEVSSRPGLGSTFRIVLPVQVPDAPPPEKVFPP"
     gene            17659..19005
                     /locus_tag="PLH85_11300"
     CDS             17659..19005
                     /locus_tag="PLH85_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB65197.1"
                     /translation="MTAPPLSTAAMPPSPTTTATHTLLLVDDEPAILSALRRLFRLAG
                     YRILTAESGAEGLELLAAQAVDLVISDMRMPEMDGATFLAAVRQHSPETIRILLTGYA
                     DLSSTVAAINEGEIYRYVAKPWNDQDLTLLVHDALERRALLRENARLDAVTRAQNEEL
                     RALNAGLEAKVAERTAALSAALGRADEAHRQLRQSFTAMVRVFSELSEAGTASAGHGR
                     RVAELARSIARQLQLDDAAQQTVMLAGLLHDLGKVGLSAELLAKSRARLSADERTVLM
                     HHPKRAESLLTGIPSLRDVARLIRHHHESFDGSGFPDRLVGLAIPLGSRILCVANDYD
                     GLLRGTSVAGRPLSHRETVEFMLANRGKRYDPPVVEALVNVVAGSEAVADDLAVNTAQ
                     LRVGMVLSADLVHPEGYLLLARDFPLDAAMIAQLQRLETSEGLRLQLRVRRGDDAR"
     gene            19002..20450
                     /locus_tag="PLH85_11305"
     CDS             19002..20450
                     /locus_tag="PLH85_11305"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNB65198.1"
                     /translation="MTRADGTLAPFQWASYFETGCADVDAQHRKLVDLVNALAARAAG
                     SGALNAAELGHILDGLGQYARHHFATEEALMERAGLDPRHVDAHRAAHADFVEQVGAL
                     AGAGESDNALPRLYEFVCAWLTYHILDTDQAMARQLRARAAGVEAAAAFDAEGAGTAD
                     PGNAALIVAVRSLLGVIAGRNAALARANADLERRVEARTAELATAQARLREADTLAAV
                     GQLAAGVAHEINNPIGFVTSNLSTLATYAEQLLTLTAAADGLAARHATDSAWQAARQT
                     VDVDFIRDDLSALLDDASRGLERVREIVQALQAFAAPSEVRFTPIALAPLLADLCRSV
                     PTAAGQTVRWETCDTPPPVAGSAPQLREAFRALLDNGVKALAGQPGTVSVRCTSETAA
                     IRVDVEDDGCGMAPSVRAHIFEPFFTTRPVGSGRGLGLSSAYRIFRQHGGRIEVRSAP
                     GQGSTFQVWLPRLPAPDAAAPAEHEKARPEPG"
     gene            complement(20473..>20570)
                     /locus_tag="PLH85_11310"
     CDS             complement(20473..>20570)
                     /locus_tag="PLH85_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNB65199.1"
                     /translation="NPLSGHGLAGLFSTHPATEERVARLRSLATR"
BASE COUNT         3257 a         7224 c         7078 g         3011 t
ORIGIN      
        1 ggcgggcggg tgacgaacga gatgtatgca gtggcagggg ggatgggggg tgcgggataa
       61 aaccgcggga acgatcagat caggctccgc cagagtcctc gaacgcttga acccgccgcc
      121 gatccccctt ccattagctt agtaacacac ttgataaaca ggcatgacac gtcccatgaa
      181 tacgccccca ccaacggtca cacaagtgga tcccgtatgc cgcacgaccg ccacagactg
      241 aacgtccaaa atggtcgtat cccgggcttt aggacactcc tggatttcac cctgggtggc
      301 gatttctggg gctttcgcag taggcgtctc aggcgccttg ggcaataggc agggctgaat
      361 ccgtttgcct tcggcgctaa cggggagcac ttcgccattt ttgccaatca ccaacgcgcc
      421 gactgcatag gatgggtcca cgaaacgctt cgcaacatcc ttcaggtcgg gctcggggat
      481 cgaggccgcc ggtgcaggag gcttgggatg ctcacatccg gtcagacctg caagaacggt
      541 gactccaaac gccgccgcaa gggcggccgg tacaacgcta atgcgtgtaa acatggcttc
      601 taaactccat agaacaaaag attcttcaat cagtaacgca gggtcagctg gaccaatacc
      661 gtgcgtcgag gatagaaaag aggggccttt ggatccccgt tgtaatcagc attctgaaat
      721 ctgaaccgac gatcaaacac gttgctcgcc cccaggcttg caacgacgtc cttcccgatc
      781 aagggcatgg agtatcttag gttcatgaca gaaaaagcgg aatgccccac cagatcggag
      841 cctgtgaagg catccggcat ccgcgcattt tgccggacag cccaattttc cagattaatt
      901 gatccggtac ccgccttgac ccacagacga atcggcacga ggtccgtcct gagccgatag
      961 gggaacatcg acaattgcct ggggtcgtca ttgatgtggc tattctcata acgccattcg
     1021 gccgaagcgg cgacccgggt cccaaaaggc agggccatat agacgcggtg cagatcctca
     1081 tgccagtgag cgaggcacgc ttcgcctgta caacccagtc cagggacatc aaggtgcctc
     1141 gacgacactt ccgcccccag cgcagcgccg cccgcgaaat gctgatcgac cgccaaggcg
     1201 ccacgcgtcc agcgcgttcc gttgaaatcg tcaaagacct gattgaaccc gttgaattga
     1261 gttggctcca gtccttcccg atcatatttg gaacccttta caccttgaaa tagcgccgca
     1321 cgaaccattg tcccggtcct aggctgtagc agcaggccga tttttccgct gaatctttca
     1381 gccttggtga ctgtcggctc gtcgtagcgg gtgtattcgg cgccggtcag aaatgttacg
     1441 accggattga ggcgccaccg actgtaccca aaggctgcat cgcggcccgc ccgcgttgaa
     1501 aacgtggacg caaaaaccgg aaagaccccg aatccgggaa tcgagagatc gaagttctga
     1561 tctgctgtac cgctcaactt gaaactggtg actccggcaa tcagggcact ctcggctcgt
     1621 tgaacggaat acatcgcgga aaaacggcgt gcattcagtc gcgcagaata accattgagg
     1681 acgtcgtaag aaccattcag ggggtcgtcc acatgggcac tttgcgatga ccgatcgcga
     1741 atctgctgag tgcttgcctc cagcaggagc tcttccccaa tgccccgtga catacggaac
     1801 gccacgcgcg cgaggtccga attgactccg tcacgcagac gcttgaggtc cgcatcaaac
     1861 agactcagcg tctgatcccc gctcacccgc tccgtatgcc gcagttccgc aaacacgctg
     1921 ctggccggcg tcagggcgaa ctggccggtg aggcgggtgc cggagagatg ggtgtcggcg
     1981 tcgccggcca cgagggcgcg gctgcggtag tcgaaggcgc cgacgccgat ctggccgcgc
     2041 tcccaggcat gggcggcgac gaggctggcc gcgccggtgt cctgattgcc gccaagggca
     2101 gaaaacgcaa agtggtcggg tttgcgcgca aacaggccgg tgctctcgtc cggcgagagc
     2161 gcacgggggc cgtccagcac cggcagggcg ggcatcacaa actggggcgg caggggcatc
     2221 tggccgatgg gctggcgcag ctcggcttgc agcagctcgc tgacgcgcgc ggtctcgaag
     2281 cgcggcacgc cggcataggc gtcggcgagc aggcggtggg cggccgggct ctggggatcg
     2341 tccgccaggg catccatggc acgggcctgc atggggccgt agaagccgac ctcgcggtag
     2401 gcttcaccga gactggcgct gcgggcggcc cggtcctggt cgaggagttc gccggaacgc
     2461 agtacgccgc gggactcgtt gcgcgcgacg gcctcttcgc catcggcaac ggcggcgagg
     2521 aaatcccggt tgcgcagctt gcggaaggcg tcgaaatacc agggtgtggg cgatgcgggg
     2581 tcgaggcggc gggccagctc gaactggtcg ccggccacct tgtcccgatc ctcttccagg
     2641 taggcacggc cgagggcgct gcgcagctcg gcgttggagg gatcgaggag gacggccacc
     2701 tcgagttcct gccggcccgc ggccacctcg ccctggcgca ggtgggccag ggcgcgaccc
     2761 agatgggcga ggggctcgct gccgtcgtcc gcgagcagcg cgtcgaaggc ctccgcggcg
     2821 gcttcggtgc gtccggcgag gagttctgcg aatgcggcca gcgcccgggc gcggggcacc
     2881 gcgggattga gggcgagggc acggcgcgcg gactcgcgac cggcggcgag gcgcgacagg
     2941 gccagctcca gttcggcgcg acgcgcccag gcgaccggat cgtcagggcg cagggcggtg
     3001 gcggactcgg cggcagcaag ggccgccggc acctgacggt cggcctgcag ggcatagctg
     3061 agcgccagcc gggccggcgc ggaggccgga tcgctgcgca ccgcatcgcg ggccgtggca
     3121 agggcctcgg cgtcgttgcg ggccacctgg atgaccgccc ggacggcggc cacgccggcc
     3181 tccggggtgg ccgcggacag cacgccgcgg gcttcatccg cccgaccgag cccaaggaac
     3241 agagcggccc ggaaggcggg cggtccaccg tcacccagag tgtcgagcgc cgcccggaag
     3301 cgccccgcct cggccagact caccgccgtg cggaggacgg gatcggccgc cagtgcgtcg
     3361 gctggcgggc gcgccacctg gggatagtgg atcgcccagc ggaccgcgtc gaggggactg
     3421 acgaccagcg ccggaccagg cgcggcgccg tcggccacat cggtcgcctc gccagcaccg
     3481 aggctaagtt cgccggcgcc cgtcaccgcg cgcacgtggc cttccgccac actgacccgg
     3541 gcccgtccat ccccatcggc gacgacgaac tccgtgccat ccacgagggc gttgacgacg
     3601 ggcgtcagca cacccaggcg tttgcggaag cggctgatga catggaccac gccccccgtg
     3661 aggccgagct cggtgtccgc gcgggtgggg gacccaggca acgtgagggt ggtgttctga
     3721 tcgaggcgga cgagcacgtc ctgggcgagg gcgatggcgg cgcgaccggg gccgcggacg
     3781 gccacctgat cgccgggaca cagcatctgg cggagaccgg cgggttgcca cggaccatgg
     3841 acgccggcgc ggacctcgac cagaccttcg atggcggcga gacgcccggc gggttgatca
     3901 caggccgcgt gcgccgcgcc cgcaaaggcg attgcacagg ccgccacgac ccaggcatgc
     3961 cgtccgctcc gttcatccga tgcccgtcga ccgtgcccca tgatatgccc cgccatggca
     4021 acgccttgat tcggaaaagt tccgcgcgat gttatcgcaa aaggtgacga caaccatgcc
     4081 gtccgtcggg gatttctgcg ggataccgcg atgccgcagg tctacttcga tccgagcacg
     4141 acgatgccgt ccggatcggc gccgagggcg ccacgctcac ggcagcgggc ggcatagaac
     4201 cgggccgggc cgtcccccgg gacgagggtc agggtggccg cgaagcgttc gcgcgcccag
     4261 tccgcagcgc ccccggacca ggcggcgagg ccgtccgcaa aggcccggtc ggcgtccgcg
     4321 gggagggcct cgcagcgcag ttcgtgcagc ccgaccggcg cagactgtcc cgccagcacg
     4381 aagcggccga ggggccgcgc actgcccggc cccccgtggg cagtcacctc ggcagacgcc
     4441 aggatgcgcg tgcccagatg cttgttggcg ctctggatgc ggctggcggt attgacgatg
     4501 ttgccgacgg cgcggatctc ccggtggtgg gtggcgccca gttcccccag gaaaaccggc
     4561 cccacatgca gaccgaagcg ggttggcagg gtgccttcgg cgggcgcacg gttcatcagg
     4621 gtgtggagct cgaggaccgc cgcgcgggca cgggcgcagg cctcgtccgg agcatcatcg
     4681 gccagccaca gggacagcac cgcatcgccg acgatgtcca tgacgtgccc gccgtgggcc
     4741 tgcacgacgg ggatgaaggc cgcgaagtag cgattgagga tgtcccgtgt cgcctcgggg
     4801 ctgaggtttt cggcgagcgc cgtatagcct tcgatgtcac tgcacaggca cacaccatga
     4861 aacctgcgcc ccgcctcacg gccccgcagc agggacgcca gggcctcgct gcccttgtgg
     4921 gtgaggccca gatccacggc acgcctcagc tgacgttcgc gccgccgtgc cgcgcggatg
     4981 tgccacgcga tgcccagcag tgcggcgaca gccggcgcga ccagcccggc cagaatcacc
     5041 ggcagccaca ggtcgaggac cgcaaaggcc caggccgacc ccgccacggc gaccccgccc
     5101 aggatcgccg tgaccagcgc cgcgcgacgc gtccccagcc ccagccaggg cagactgagg
     5161 agggccgccc aggcgagcag gggcggcacg gtggcggcgt cctccggccg tgtcagaccc
     5221 gaaccgtcca gcaggttgcc gagtgccgtg gcgaggagct cgacgcctgg cagatcgacg
     5281 ccatcctcgc gggtgaaggg cgtgcgatag gcgtcggcct ggcgggactg gttggactcg
     5341 gcgaagccca ccagcacggc cttgccggcg aacgccactg caccttcctg cgggtcggcg
     5401 gccagcgcca gggcacgggc ataggagacg gtgcggacac tgccgagggg accgtagaga
     5461 ttgaggacgc gacggcgggc ggccggtgac ggggccgctc cgatccgttc cgccatcagc
     5521 accggaaggg acggctggtc accggcgctg ggcagggaat cccagaattc gaagaccccc
     5581 tccggcgtct tgggcaggac gaaggggccg ctgccggcga ccgcatcgag gaacaggggc
     5641 agggcacgta cgcgccggtc cacgctgccc agcgccgtgc catccggcgc gctgatggtt
     5701 tcgcgggtga gctgctccac cagcatgacg ttgccggcac ggcggaacgc ggcggccagc
     5761 gcctgatcgt cctcggcggg ctcacgggaa cgttcgaaca gcacgtcgaa gccgaccacc
     5821 gccgcgcccc gctcggccag cagcccgacc agcaccgcgt gcaggctgcg cgcccagcgc
     5881 tcggggcgct ccgactgccc gagggccccg gcggacgtgg cgtcgagggc tataatcatg
     5941 acccggtccg ggggcggacg gatgccgcgc agggcgtaca gcagacgcag gccggcgccc
     6001 tcttcccagt cccaggccga tgtgagcagc accacgccca tggccgcggc ggaggccagg
     6061 ggcaggacgg cggcacgcaa ggaacgggcg aggtggcgga tcacggcgga atcatcgagc
     6121 ggggagaagc attgtgaact atctggatcg ggtcgatctg ttcgcgggcc tcgacgaggc
     6181 ccagacgcag agtctggtgg atcgctcgcg tctgcgcagc ttcgtggcga gcacggtggt
     6241 cgtcaacgag ggcgacgagg gcggcagcat gtacgtggtg cagagcggct ctttgaaagc
     6301 ctatctcacc gacaagacgg gacgggaggt caccctgtcc ctgctcgacg aaggggacta
     6361 cttcggcgag ctggccctgc tcgatgacgc gccgcggtca gcgtcggtgg cggcggtgac
     6421 gcgcagcgag ctgctgcagg tgccgcgctc cgccttcctg gcgctgatcg aggagcaccc
     6481 ggccagcatg caggtggtgg tacgcaatct ggtggcgcgc atccgtgccc tgtcggacaa
     6541 cgtccgctcc ctcgccctgg aggacgtctt ccggcgcgtc acgcgggcct tcgagaccct
     6601 cgcggtggag cgcgacgggc aacgcctcat cgagcgacgc ctgacccagc aggatgtggc
     6661 cagcctggtg ggcgcgtcgc gggagatgat caaccgggtg ctgcgggagc tggtggccgg
     6721 cggctacgtg gagattgaac agcatcacat cgtgctgcgg cgcaagctgc ccacctgacc
     6781 gctgcggagc gcccattccg ggtactctga ccggaaaaac cgcacggttt gcatggtcat
     6841 ttttcacggg cggactacac tgaaataaac acgaccgggg agacgatcat gtgcttcgta
     6901 gattcgccca tcagtgcctg caacgccgtc cgcgagatgg tgctcaccga tcagacccaa
     6961 cgggaatgcg cccgcgagca tgactgcccg cccggtcgca actgtccgct ggaaggctgt
     7021 tttgccgaaa tctcgggcca gagcgaggag gccacccgcc gcgaagtggc cgcggcggtc
     7081 gccgcccatc acgcctgaga tggccaaccg gacgcttggc acgcgccaga aacaggtgtt
     7141 ccgcggcccc gccaagggcc ccgcggggcg ggtggtgccg cgcaacccgc tggtgctgcc
     7201 ggcggcccag cgcaaggccg ggccccatgg caagtcggaa agcgccaagc ggcaggcggc
     7261 cgagcgacgc ctgaaccgcc agctcgaaaa gctgaccggg gagggcgacg gctgaacagc
     7321 cgccaacgcg cgccgtcaac gcaggcgttc gagcagaccg tcaagctggt ccagggtgcc
     7381 gaagtggatg gtcaccatcc cggcaccctt tttattggcc ttgatcttca cggtggcgcc
     7441 gaggcggtcc gccacctcct cttccagacg caggatgtcc cggtccggcg ccggagcggc
     7501 gctctgcggc ttggggtgca gcgcctggta ggcgaggcgc tcggcttcgc gcaccgacag
     7561 gcccttggcg gcgatcacgt tggcgaggcc gacctgcgtg gcgcccggca gaggcagcag
     7621 ggcgcgggcg tggcccatgt cgatgtcgcc agccatcagc agttcctgca ccggctgcgc
     7681 cagctgaagc agccgcagca ggttggaggc ggccgggcgg gagcggccca ccgcgtcggc
     7741 ggcctgctga tgggtcaggc cgaactcgtc cacgaggcgc tggatgccgg cggcctcttc
     7801 gaggggattg aggtcttccc gctggatgtt ctcgatgagg gacatggcca gcgcggcctc
     7861 gtccggaatc tcccgcacca ggcagggcac ggcctccagg ccggcgatgc gcgaggcgcg
     7921 ccagcgccgc tcgcccgcga tgatctcgta gcgctgggcg tcgaacaggc tgcccaccgg
     7981 gcgcaccagg atgggctgca tgacgccctg ggacttgatg gacgcggcca gttcttccag
     8041 cgagccgggg tccatgcgcg tgcgtggctg gtacttgccg ggcttgaggg cgtcgacggg
     8101 cagcgtctgc agttcgcctt cggtcgtctg ggcgtcatcg ccgttgccgg acagcagggc
     8161 gtcgaggccg cggccgagac ctttgagttt gggaggtgcc atggtgtcgt tctcgctgga
     8221 ggcgccgggc agaggcgggc gccggtccgg tctcagaggg tcttgatgcg ttcgatcatc
     8281 tcttcggcga aggccaggta ggcctgggcg cccttgctgc cgcggtcgaa gacgacgccc
     8341 ggcaggccgt ggctgggcgc ctcggcgagg cgcacgttgc gcggcacgag ggtgcggaag
     8401 accttgtcgc cgaaatgctg ttcaagctgg gccgagacct gttgctggag cgtcacgcgg
     8461 ggatcgaaca tgacccgcag caggccgatg atcttgaggt cgcggttgag gttggcgtgc
     8521 accttcttga tggaattgac caggtcggac aggccttcca gcgcgtaata ctcgcactgc
     8581 atcgggatga tcacgccgtg ggcgcagcac aggccgttga gggtcagcag ggacagggac
     8641 ggcgggcagt cgatgaggat gaagtcgtag gtgtcgccgt ggctgccgag ggcgtcgcgc
     8701 aggcggttct cgcggcgcgg cagggccacc agctccacct cggcgccggc cagatcgcgg
     8761 ttggcgggca gcacgtcgta cttgcccgcg gcggaggcca cccgcgcctc ggccagcgtg
     8821 gccaggccca ccagcacgtg atacacggag cgccccagac tgcgcttgtc gatgccgctg
     8881 cccatcgtgg cgttgccctg ggggtcgagg tcgatcagca gcacccgctg cccggccgcg
     8941 gccaggccgg cggccagatt gacgctggtg gtggtcttgc cgacgccgcc cttctggttg
     9001 gcgatgcaga aaatgcgggc catggttcag gtccgtttca cgatgaggag gtggcgcgcc
     9061 gcgtcgaggc ccggcacggc gagttcgagg cagcgttcga ggacgaagcc ggcgggcaaa
     9121 cgggcgatct cgtcggcagg atagacgccc ttcatggcga acagacgcgt gtccggcccg
     9181 gccagatggg ccgtgagatt cacgaagtcc gccagatcgg cgaaggcgcg ggagatgatg
     9241 ccatccggcg cggcgtccgg cccgtagggc ggctgccagg actcgatgcg ggcgtggtgc
     9301 gcgcggaagt gctcgagctt cagttcgatg cgcgcctggt tctggaaggt cgcctttttt
     9361 tgcacggtct cgacggaatc cacctgcaga tccggccggg cgatggccag tacgacgccg
     9421 ggcagaccgc cgccggagcc cacatccacc agccgcgtga cgccggccag gtggggcagc
     9481 acgctgaggg aatccagcag gtggtgggtg acgacctggg cctcgtcgcg cagggcggtg
     9541 aggttgtaca ccttgttcca cttgatgagc agacgcgcga aggcttccag gcgggccggc
     9601 gtgtcggcgg gcaggtcgag gccgagggcg gcgatgccgt cggcgagggc gcgccccatg
     9661 ctcatgcgga gcgtcgttcg ccgtcgcggg cgagatcctg acgtttgagc cacaccagca
     9721 gcaggctgat ggccgccggg gtgatgccct ggatgcggct ggcctggccg atggtctcgg
     9781 gccggtgctg attgaggcgc aactggactt ccttggacag gccacgcacc gtggtgtagt
     9841 ccaggtcggc gggcaggcgg gtggcctcgg cgccggcctg gcgggccact tcgtcctgct
     9901 ggcggtcgat gtagccctgg tatttggcgg cgatctcgag ctgttcgacc acctggggat
     9961 cggtggcgag gtcctccggc gcgccgggca ggcccatcag cgccgcgtag ctcacgccgg
    10021 ggcggcgcag caggtcgaag aagctgtatt cccgctcgat ggccttgccc agcaccgcct
    10081 cggcggcctc ggccggcacc ttggccggcg tcgcccaggt ggcgcgcagg cgggcagtct
    10141 cccgctcgat ggcctcgcgc ttggtgcaga aggcggccca gcggacgtcg tccaccaggc
    10201 ccagctggcg gccggtctcg gtgagacgca ggtcggcgtt gtcctcccgc aggctgaggc
    10261 ggtactcggc gcgggaggtg aacatgcggt agggctcgga gacgccgcgg gtgatgagat
    10321 cgtccaccag cacgcccaga taggcttcgt cgcgacgcgg gcaccaggcc tcccggccct
    10381 tcacctggag cgccgcgttg aggccggcga gcaggccctg ggcggcggct tcctcgtagc
    10441 cggtggtgcc gttgatctgg ccggcgaaga acagcccgcc gatggacttg gtctcgaggc
    10501 tggccttgag gttgcgcggg tcgaagtagt cgtactcgat ggcgtagccg gggcgcagga
    10561 tggtgcactg ctccagcccg cggatggagc gcacgatggc cagctgcacg tcgaagggca
    10621 ggctggtgga gatcccgttg gggtagatct cgtgggtgtc caggccttcc ggttcgagga
    10681 agacgtggtg gctgtccttg tcggcgaagc ggtggatctt gtcttcgatg ctcgggcagt
    10741 agcgcggacc gacgccttcg atcacgccgg agtacatggg cgagcggtcc aggttggcgc
    10801 ggatgatgtc gtgggtgcgc gggttggtgt cggtgatcca gcacggcagc tggtgcggat
    10861 gctggccggc gtggccgagg aagctgaaca ccggcaccgg gtcgtcgccg ggctgctcct
    10921 gcatcaccga gaagtcgatg gtgcgggcat ccaggcgcgg tggcgtgccg gtcttgaggc
    10981 ggccctgggg cagggcgagt tccttgagac gctggcccag gcggatcgcc ggcgggtcgc
    11041 ccgcacggcc ggcggagtaa ttctgcaggc ccacgtggat caggccgttg aggaaggtgc
    11101 cggcagtgag caccacggtg ggcgcctcga agcgcaggcc gatctgggtg accacgccgg
    11161 taacccggtc gcccgccacg gtgatgtcgt ccacggcctg ctggaagagc atcaggttgg
    11221 gctggttctc caggcggcgg cggatcgcgg ccttgtacag cacccggtcg gcctgggcgc
    11281 gggtggcgcg caccgccggg cctttgctgg cattgaggat gcggaactgg atgccgccct
    11341 cgtcagtggc ggccgccatg gccccgccca gggcgtccac ctccttgacc aggtgcccct
    11401 tgccgatgcc gccgatggac gggttgcagc tcatctgccc gagggtctcg atgttgtggg
    11461 tcagcagcag ggtggcacac ccggcccgcg ccgccgccag ggcggcctcg gtaccggcgt
    11521 gaccgccgcc gacgacgatc acgtcgaaac gggtgggata gagcatggac aaagcgggat
    11581 ggggattcgg gggatcggaa tgatacgacc ttgcgctgca aaaatacagc gccgcggcac
    11641 ggcctccaga ataaaatcag cggttttaca acagcttagc actcattccc ggcaccatga
    11701 cgggggtccg gacacgcccc ttgaggcctc tgagcagcgc tcgatgacac gggcgcagat
    11761 gggcattccg gaccccacgg cggatcggcc ggaccggagc ggctacgcct gcggaatatc
    11821 gacctatacc taaagtcact ccgacctccg ccgattaagc cggagggcgt acgtcaatcc
    11881 tgctggcgcc cgcccccatc tttctgcggg acatgaaacg cggcttcacc acccatctcg
    11941 tcatctgggc catcgtgctc ggtctgggcg cctacctggc gggcaccctc gtgcacacgc
    12001 gcggcgcggc gcaggcccgc gcccgcaaca cggccctgac ccatgcccgg ctggtggccg
    12061 agcacgccca cgcgagcatc cggcaggttg aactgatact cgacgatctg gccgagcgcc
    12121 tgccgcgcga cgcgttggcc cagggcaccg ccttgccgga catccgtcgt gccgccctgc
    12181 gggatctcct gaacacggcc cgcggacgca tggcgggcat cgaaaacgtg atcctgtcgg
    12241 acagtgtcgg catgctgacc gtcaccgcca tcgaccccgt cccgatggtc gccatcgacg
    12301 accgcacgta tttccgcacg gtccgcgagg gtgcgccggg gcagaccttc ctgtcggacg
    12361 ccgtccaggg gcgggtgtcc ggcaaatggg ggctgatggt ggcccgtcgc ctggaagacg
    12421 cgagcggacg ctttctcggg ctcgtggccg tcaatctggg gctggaccgc cagttcatcc
    12481 acttctatcg ctcgctggcg cccgagggcg acgcggcaat cgccctgtgg agccacgact
    12541 ggcgcctcct cacccgctac cccgtcgtcg aaccggagat cggcaaggct cacctgatcg
    12601 agtcgctggc gcccctgatc gacgctgacc tgaacgagac gtccacggtc gccgtctcgc
    12661 cggtggatcg tacggtgcgc ctgatcgccc tgcgccgggt ggaggactat ccgctgtacg
    12721 cgagctttgc ggtcgccgaa tcggactaca tggcccagtg gcgcgaccag gcccgcgaca
    12781 cgatgctggc gatccttgcc ctgctggtcg gtggcggcgt gctcagcacc ctggaggtcc
    12841 gcagcggccg caccgccgag cgggtgcgcc gcctgatccg cgaaaccccg ctcggactga
    12901 tcaccctgcg ggccggcgcc gaaggcgatc tgctcctgct ccagtccaac ccggcggccc
    12961 accgcatcct ccagaccaat ctcgaacccc acgccggcga gcgtctggtg agcctgctgc
    13021 ccggtctgac cggttcgccg atcctggagc gggtgcgcgc agtggcccga cagggtcagc
    13081 cctggcgggc cagcgggctg ccctaccggg acgagcgcct gcaagcactc ttcgacatcg
    13141 tggcgttcca ggtggcgccg ggcgtcgccg gcgtgatgtt tgacgacgtg tccgagcggg
    13201 aagcggccgt ggcggccctc aacgcccgcg aacgagacct gcgccgtgcc gaatccctcg
    13261 cccagctggg gagctggcgc ctgcgcctgg ccgaccgcag catgacctgc tccgacgaat
    13321 gtctgcgcat cctcggcgcc cgcgccggca tgccggtgag cgtgtccacc ttcaccgcgc
    13381 tgatccaccc ggacgaccgg gaacgggtcc gcgcggcgtg gcgcaacctg ctcgccggcg
    13441 ggcgcatgga cgagacttat cgcatcggcc gcgagggccg gcccaccacc tgggtgcgtg
    13501 cgcgggccga acacgacccg ctgcccggcg acgacatcac cctgctcggc accatccagg
    13561 acatcaccca gcagcacgag gccgaacagc gcctgaagga cagcgaagac cgcttccacc
    13621 agctcttcgc ccgtcacgcc tcgatcatgc tgctcatcga gccggtcagc ggcgccattg
    13681 tcgatgccaa cgcggcggcg gccgagttct acggctacgc catcgagacg ctggtgggca
    13741 tgcgcatcca ggacatcaat gccttgccgc cggaagacgt agagcgggaa cgccagcggg
    13801 cgttcaagga acagcgcaac tacttcgtct ttccgcaccg gctcgccgac ggacgcctgc
    13861 gcacggtgga ggtgtattca tcgccaatcc aggccggcgg ccagaccgtg ctgttctcca
    13921 tcatgcacga cgtcaccgag aagcagctgg ccgaatcgcg cctgcgcctc gccgccgggg
    13981 tgttcgacaa tgcccatgaa ggcatcgtcg tcaccgacct gagcggcacc atcctggagg
    14041 tgaaccgggc ctactgcacc ctcaccggct acgagcgcga agacctcatc ggccgcaatc
    14101 cgcgcatcct ccaggccggc ccctccgacg aggcgttcta cgccgagatg tggggccacc
    14161 tgggccggcg cggcttctgg cgcggcgacc tgcacaaccg ccgcaaggac ggcagttact
    14221 atctgcagga aaccggcatc accgcggtga ccaatgccct gggcctgcgc acgcactaca
    14281 tcggcttcgc cgaggacgtc accctgctgc gccagagtca gcagcgcctg gaatacctgg
    14341 cctaccacga cgccctcacc cagctgccca atcgattgct gctggccgac cacatccgcc
    14401 tggccctcgc cgccgcccgc cgcgaagggc gtcacgtggc cgtgtgctac ctcgatctgg
    14461 acgagttcaa gccagtcaat gaccagtggg gccacacggt cggcgaccag ttgctggtag
    14521 aggtggcggg acggctgaag gcctgcgtcc gcgccgaaga cacggtggcc cgcctgggcg
    14581 gcgacgagtt cgtgatcctg ctggccagcc tggacagcag gaccgaaggc gaacaggccc
    14641 tcgcccgggt cgtcgccgcc ctatcgtccc ccttccagct caccgtcggc gagatcggcg
    14701 tcggtgcgag catcggcgtg agcgtcttcc cgcccgacga cgcagacgcc gattccctga
    14761 tccgccacgc cgaccaggcc atgtacctgg ccaagcaggc cggccgcaac tccatccgct
    14821 tcttcgaccc ggacgccgac cggcgcgccc gcagcctgcg ggattcggtg gccgaggtcg
    14881 cgctgggcct ggagcggggc gagttcgagc tctactacca gccccaggtc catctgcgca
    14941 gcggccgggt ggtgggcgtc gaagccctgc tgcgctggaa ccatccccaa cgcggactgc
    15001 tggcccctgg cggcttcctt gcagaggtgg acaacaccga agtcgccgtg gccctgggcg
    15061 agtgggtgct ggacacggcc ctccgtcagg ccgacacgtg gcatcgggcc gggctcgaac
    15121 tgcgcgtcgg catcaacgtc tcggcacatc acctgcagca ggacaccttt gccgcacgcc
    15181 tggcggaacg cctctcgcgc caccccgagt tgccggccgg ccgcatcgag ctggagatcc
    15241 tcgagaccgc cgccctcgaa gacctcagcc gcatctcgcg gctgatggaa acctgccgcc
    15301 gcttcggcgt ctccttcgcc ctcgacgatt tcggcaccgg ctacgcgtcc ctcgcctacc
    15361 tcaagcatct gccggccaac ctgctcaaga tcgaccagag cttcatccgc gacatgctgt
    15421 cggatccgga agaccacgcc atcgtgcagg gcatcgtcgg cctcgccatg gcgttccgcc
    15481 gcgaggtcat cgccgagggc gtggagagtc tggcccacgg cgaggcgctc ctcgccctcg
    15541 gctgccccct cgcccagggg tactgcatcg cacgccccat gccggccgcg gccgtcgcgc
    15601 cgtgggttgc ccactggtgc aacccgccgg aatggtcgca gccggcgccg ccagaggacc
    15661 ggccatgaag cccctgtgcc cgcttccacc tcccgtggcg gggctggccc cgcccctgcg
    15721 ggcctgtgac ccctggctcg tcgtgagcgc cgatgcccgc cgcgcggccc tcagcgaggc
    15781 cgcggtccga ctgctggaac tgcccgacgc ccacgccgaa ttgcccgcca cgatacagac
    15841 ggtcctgggc caggccgacg gcgatttcgt cctcagcctg gatggcgccg acggggcacg
    15901 cttcctcagc gtggcgctcg gcccggatcc ggacgcccgc ctcggcgcgg gcacccgcct
    15961 cgccgtcctc cacgacgcaa gcgggctgat ccgcctgcag cgcgccctcg ccgacagcga
    16021 ggggcgcctg caggccatcc tgggtgcggc gatggacgcc atcatcgcca tggacgaggc
    16081 tggccgcatc accttgtgga atgccgcggc cgaacgcctc ttcggctggc gtgccgacga
    16141 ggtgcggggc cgcgacctcc acgagaccct ggccgggacg gcggagcggg cccgcttcct
    16201 ggcccatgcc ggacgctgga cacggggcgg caccagccct gtgttcggac agatcctgcg
    16261 cctgcccgcc caccgccggg acggcaccgc cctcgacatc gaactgtccc tcggcgcctt
    16321 tcgccgccat ggccactggc aggccctcgg catcgtccgc gacgtaagcg cacgggtcgc
    16381 cgccgaactg gagctgcagg tcaccggtac ccgcctgcag caggccctcg aaggcggcaa
    16441 catcacctat tgggactggg acgtgagcac cggcagcctg gtcctcgggc cgcgctactt
    16501 ctccatgctc ggctacccgg acgacgcctt tccccacact gcggagagct tccgccagct
    16561 cgtccatccg gacgatcggc cgcaggtgga ggcggcgctg aaggccgtgc tgaccgggca
    16621 gacggccgag ttcgcgtgcg atttccgtct gcgcgccgcc gacggcaccg accgctgggc
    16681 caccggccag ggcaaggtca cggcccgcga cgccgccggc cggccattgc gggtgatcgg
    16741 cacccaccgg gacatccatg cctttcacga gagcgaagcc gccctgcagc gccagctcgc
    16801 cgagaccctg cgcctccacc gggaactgga ggaagcccag gtgcagctgg tgcagagcga
    16861 gaagatggcc gccatcggcc agcttgccgc cggcgtggcc cacgagatga acacgcccct
    16921 gggcttcgtg aaatccaacg tgggcaccct gcagacctac atctcgcccc tgctggaggt
    16981 catcaccgca ctgatggcgg ccaccgacgg cctccccggc ggggcggcgc tggccgacgc
    17041 ccgcgcccgc gccgccgccc tcgacctgga tttcctgcgg caagacctgc ccgacctgtt
    17101 cgcagagacc cgcgacggca tcgtccgggt ccaacgcatc gtcgccgacc tcaaggactt
    17161 ttcccacgtg gacagcgcga cctgggccca cgccgatctg caccggggcc tcgactccac
    17221 cctcaacatc ctccacaacg acatcaagca ccgcatccag gtgatccgcg actacggcga
    17281 cctgcccctg ctgtggtgcc gcccgtccca gctcaatcag gtcttcctca atctcctcgc
    17341 caacgctgcc caggccatcg ccgaccgggg cacgatcacc gtacggaccc gtgccgacgc
    17401 ccgccatgtc acggtggaca ttgccgacac cggctgcggc atcgccccgg agcacctgcc
    17461 acgcatcttc gacgccttct tcaccaccaa acccgttggc aaaggcaccg gtctggggct
    17521 gtcaatcagc ggcgacatcg tgcggaaaca cggtgggcac ctggaagtga gcagccggcc
    17581 cggccttggc agcaccttcc gcatcgtcct gcccgtgcag gtgccggatg ctccaccgcc
    17641 cgagaaagtc tttccgccat gaccgcaccg cccctttcca ccgccgccat gcccccctcg
    17701 ccgacgacca ccgcgacgca caccctgctg ctggtggacg acgagcccgc catcctgtcg
    17761 gccctgcgcc gcctgttccg cctcgccggt taccgcatcc tcaccgccga gagcggcgcc
    17821 gagggactcg aattgctggc cgcgcaggcg gtggacctgg tcatctccga catgcgcatg
    17881 cccgagatgg acggcgccac cttcctcgcc gcagtccgcc agcactcgcc ggagaccatc
    17941 cgcatcctcc tcaccggcta cgccgacctg tcgtccacgg ttgccgccat caacgaaggc
    18001 gagatctacc gctacgtcgc caaaccctgg aacgaccagg acctgaccct gctcgtgcat
    18061 gacgccctcg agcggcgcgc cctgctgcgc gagaacgccc gcctggacgc tgtcacccgc
    18121 gcccagaacg aagagctgcg ggccctcaac gcgggcctcg aggccaaggt ggcggagcgc
    18181 accgccgccc tctccgccgc cctcggccgc gccgacgagg cgcaccgcca gctgcgccag
    18241 tccttcaccg ccatggtgcg ggtgttttcc gaactctccg aggccggcac cgccagcgcc
    18301 ggtcacggcc gccgcgtcgc cgagctggcg cgcagcatcg cccgccaact gcagcttgac
    18361 gacgccgccc agcagaccgt gatgctcgcc ggcctgctcc acgacctggg caaggtcggc
    18421 ctctccgccg agctcctcgc caagtcccgc gcccgcctca gcgccgacga gcgcaccgtg
    18481 ctcatgcacc acccgaagcg cgccgaaagc ctgctcaccg gcatcccgtc cctgcgcgac
    18541 gtggcccggc tgatccgcca ccaccacgaa agcttcgacg gcagcggctt cccggaccgt
    18601 ctcgtcggcc tggcgattcc cctcggcagc cgcatcctct gcgtcgccaa cgattacgac
    18661 ggcctgttgc gcggcacctc ggtggccggc cgccccctca gccaccggga aaccgtggag
    18721 ttcatgctgg ccaaccgggg caagcgctac gacccgcccg tcgtcgaggc cctcgtcaac
    18781 gtggtggcgg gcagcgaggc ggtggccgac gatctcgccg tgaacacggc ccagctgcgc
    18841 gtcggcatgg tgctctccgc cgatctggtg caccccgaag gctacctgct gctggcccgc
    18901 gactttcctc tcgatgccgc catgatcgcc cagcttcagc gcctcgagac cagcgaaggc
    18961 ctgcgcctgc agctgcgtgt gcgccgcggc gacgacgcgc gatgacccgc gccgacggca
    19021 ccctggcgcc cttccagtgg gcgagctatt tcgagaccgg ctgtgccgac gtggacgccc
    19081 agcatcgcaa gctcgtggac ctggtgaatg ccctggccgc ccgcgcggcg ggcagcgggg
    19141 cactgaacgc cgccgagctg ggccacatcc tcgacgggct ggggcaatac gcacggcacc
    19201 acttcgccac cgaagaggcc ctgatggaac gcgccggtct cgacccgcgc catgtggacg
    19261 ctcaccgcgc cgcccacgcg gatttcgtcg aacaggtcgg agccctggca ggtgccggcg
    19321 aatcggacaa cgccctgccg cggctctatg aattcgtctg cgcctggctc acctaccaca
    19381 tcctcgacac cgaccaggcc atggcccgcc agctgcgcgc ccgcgccgca ggggtggagg
    19441 ccgccgccgc cttcgacgcg gaaggcgccg gcaccgccga ccccggcaac gccgccctga
    19501 tcgtggcggt gcgcagcctg ctgggtgtga ttgccggtcg caacgccgcc ctcgcccggg
    19561 ccaatgccga tctggaacgc cgcgtcgagg cgcgcacggc cgaactcgcc accgcccagg
    19621 cgcgcctgcg ggaggcggac accctcgccg cggtcggcca gctggccgcc ggcgtcgccc
    19681 acgagatcaa caaccccatc ggcttcgtca cctccaacct cagcaccctg gccacctacg
    19741 ccgagcagct gctgaccctg accgccgccg ccgacggact ggccgcgcgc catgccacgg
    19801 attccgcctg gcaggcggcg cgccagacgg tggacgtgga tttcatccgc gacgatctgt
    19861 ccgccctgct cgacgacgcc tcccgcggtc tggaacgggt gcgcgaaatc gttcaggcgc
    19921 tgcaggcgtt tgccgcgccg tcggaggtac gcttcacccc catcgcgctg gcgccactcc
    19981 tggccgatct gtgccgcagc gtgccgacgg ccgccgggca gacggtgcgc tgggaaacct
    20041 gcgacacgcc accgccggtc gcgggcagcg cgccccagct ccgcgaagcc ttccgcgccc
    20101 tgctcgacaa tggcgtgaag gccctcgcag gacagccggg cacggtgtcg gtccgctgta
    20161 ccagcgagac cgcggccatc cgcgtggacg tggaggatga cggctgcggc atggcgccgt
    20221 cggtccgcgc gcacatcttc gaacccttct tcaccacccg cccggtcggc agcggtcgcg
    20281 ggctgggcct ttcgtcggca taccggatct tccgccagca cggcggtcgt atcgaggtgc
    20341 gcagcgcgcc gggccagggc agtaccttcc aggtgtggct gccgcgtctg ccagccccgg
    20401 acgccgcggc cccggcagag catgaaaaag cccggccgga gccgggctga aacgctgctg
    20461 ggggcgccgc actcagcgcg tcgccaggct gcgcaaccgc gcgacccgct cctccgtggc
    20521 cgggtgggtg ctgaacaagc ccgccagccc gtgccccgag aggggattca
//
LOCUS       DAOQMM010000066        20494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_933_length_20494_cov_46.468027, whole genome shotgun sequence.
ACCESSION   DAOQMM010000066 DAOQMM010000000
VERSION     DAOQMM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20494
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_933_length_20494_cov_46.468027"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..517
                     /locus_tag="PLH85_11315"
     CDS             272..517
                     /locus_tag="PLH85_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65200.1"
                     /translation="MSRAYAMDGATFYQNGRKFRVQGLDVNEPGMTSEHATQRLQRAL
                     DSGNLTVDAVESDGAGHTVAVVRVNGRNVADTVRAAN"
     gene            complement(990..1838)
                     /locus_tag="PLH85_11320"
     CDS             complement(990..1838)
                     /locus_tag="PLH85_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNB65201.1"
                     /translation="MTEAIESAELHGQPVLKLQTPDGAVALVSLFGGQVLSWRPAGGS
                     ERLYLSPRAVFDGRTALRGGVPVCFPQFAACGPLPKHGFARNRPWTLVTQRSGPDFVL
                     VTLRLTDDDATRALWPHAFVAELSVGIGGGRLDLELEVDNPGDASFQFTAALHTYLQV
                     REVEATRIEGLRGLEYRDSADGDALKRETAISVTVEDEVDRIYHNAPATLLVREDQRS
                     LGIHAENFPDVVVWNPWEHKCAALPDMPADGFRRMLCVEAGAIRQPVELAPGASWWGR
                     QTLVAL"
     gene            complement(1857..2453)
                     /locus_tag="PLH85_11325"
     CDS             complement(1857..2453)
                     /locus_tag="PLH85_11325"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNB65202.1"
                     /translation="MDAAPPVRCAWCGEDPLYQAYHDREWGVPLHDDRALFELLILEG
                     AQAGLAWITILRKREGYRAAFRNFDPATVAAFDADDAARLLADPGIVRNRLKIRAAIG
                     NARAFLDLQAEFGSFDAWLWRFVDGRPIHNTWASLKDVPARTAVSDALSKALQKRGFR
                     FVGSTICYAFMQSAGLVNDHLTGCFRHAPVHALSSRPD"
     gene            complement(2453..5140)
                     /locus_tag="PLH85_11330"
     CDS             complement(2453..5140)
                     /locus_tag="PLH85_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNB65203.1"
                     /translation="MNEQHYLTPLFEPRSVAIIGASEREMSLGNVLVRNMLDAGFKGK
                     LFAVNPKHEEVLGVPCYKSVEDIPQRLDLAVITTKADRVPAIVDACGRAGTKSVVVLP
                     SGFSDAGPRGRMLEKNTVENARRHRIRLLGPNCLGILRPELGLNASFAHGSATKGSIG
                     LISQSGALCAAILDWARPNNVGFSSVVSLGTSVDIDFGEVLEYMVSDARTESIFLYVE
                     GIKDARRFVSALRAAARVKPVLLIKVGRHPAGSQAALMHSGATVGEDAVFDAALRRAG
                     VIRLYNMGQLFAAANALFSHFRPRGNRLAIITNGGGPGVMAADRAADLRIPQATLSNA
                     TITKLNEFLPQNWSHTNPVDIIGDADPERYGKTLETVLADEGVDGVLTILTPQAMARP
                     TEVAQKIIDIERAVDKPIFTCWMGEEQVREGRKLFEAAGIPTFRTTEPAVELFGHISA
                     YYRNQKLLVQTPSSLSSDLNPPSVESARLVIETALSERRKNLNEMESKALLAAFRIPI
                     AQTVVARSATEAMVLAEELGLPVAMKIDSPSITHKADTGGVRLNLNGLAAVRAAYQEI
                     LDDVKRKRPDAVINGVAIEPMVIKPYGRELMVGAFRDPVFGPVITFGEGGAHVEIQKD
                     RAVALPPLNSYLAQDMIKSTRVSTLLGEYRNMPPINMESLEFVLLRISEMVCELPWIR
                     EMDINPLIVDENGAVAVDARIVVDNIAPTADRYDHMAIHPYPSHLITKVSMPEGEITI
                     RPIKPEDAELEVDFVRKLSPETKFLRFMNTMRELPPAMVARLTQIDYDREMAFVATIE
                     ENDAEVEIGVARYAVNPDGESCEFAIVVAEDWQHRGLARRLMGVLIETARNRGLHSMA
                     GIFLSNNDRMLKFVQSLGFVLSNDPEDNTIKHGILLLQG"
     gene            complement(5387..8158)
                     /locus_tag="PLH85_11335"
     CDS             complement(5387..8158)
                     /locus_tag="PLH85_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB65204.1"
                     /translation="MENVLADALPFVAGALAGLLAGVGGILAMRSRGGSTPAETVTAD
                     GAGPHLQPPWRDFFELSSDWLWQTDAALTFVDIPGAPPSPSIAPPGIIGRNWEDVVIP
                     QLSPDFATAHRTTLERRGPITVALTLTSPTGERRHLELRGRPLFDSTGCFRGYCGIGR
                     ETSERARLESALERAEERFHAVLTHSADGYWEQDTNLRYTAVTARSGRLADLAAEHLL
                     GSRRWDLPGVSQTDAPWADHIEQIRQIASFSNFVFSRRNAAGDDIWLSESGVPVFDER
                     GLFAGYCGVTRDITAERARLLALQSRETHYRAVFDDAPSALAVIDTDGTWKSANAALG
                     RTLGVAAETLVGRPLADFQPEDDARDGQALRARILAGEPPPAGRETRFCHADGTERRC
                     RETLAWQPATEDRPDAFVAVIEDITDRHATLVALQAAESRFRQLFDQAPEAMLVVQDN
                     RIRLANLACRRLVCPPGMDGLDGLDPLELIHPDDRTMEAERLRHFLDDPTLEALPPLP
                     VRYQRLDGRPLKIEASGIRLRLDGRDALLFVLRDVARWLSAEQVLRESRAMYRDVVES
                     VNEILFQTNAAHRLTFLNRAWRHITGFDTRLSISQSLLDFVVEDDRQRVEHELMSIRG
                     GYADSARFEFRLRTQKSGPCWVEATVRPLRDSLDQSIGTSGTLDDITDRKNAEQSQRD
                     LNVELETRVRVRTSQLEASNRELEAFSYSVSHDLRAPLRAIDGFAQIVAEDYGPYLDE
                     TGREYLQRIRAASQRMACLIDDLIDLARLTRQTMKREHVNLSQIAEQIVTELRLEDPD
                     RIVDVHIEPNLYAAADRGLIRVALDNLLRNAWKFTGRRHDAQIRFHAEKRDRHLVYCI
                     SDNGAGFDMNFASKLFLPFHRMHGINEFAGTGIGLATVQRVILRHEGTIWAESTPEKG
                     ASFYFTLSH"
     gene            8446..8522
                     /locus_tag="PLH85_11340"
     tRNA            8446..8522
                     /locus_tag="PLH85_11340"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8480..8482,aa:Pro,seq:tgg)
     gene            8551..8624
                     /locus_tag="PLH85_11345"
     tRNA            8551..8624
                     /locus_tag="PLH85_11345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8585..8587,aa:Arg,seq:tct)
     gene            8676..8751
                     /locus_tag="PLH85_11350"
     tRNA            8676..8751
                     /locus_tag="PLH85_11350"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8709..8711,aa:His,seq:gtg)
     gene            9127..10176
                     /locus_tag="PLH85_11355"
     CDS             9127..10176
                     /locus_tag="PLH85_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB65205.1"
                     /translation="MASSVWWKKAFRRPPRLNLALQGGGAHGAYTWGVLDALLEAGIG
                     LEGISGTSAGAMNAVALADGWTRHGADGARESLDAFWEAVADSLPPELEWLHSLNPAD
                     DGGLPGPMRMMMGLTQYFSPYSLNPLEVNPLRDVVRARFDFERIRRDCSLKLFIAATR
                     VRTGKVRLFTTGELTEEAVLASACLPTLHHAVEIDGEAYWDGGFTANPAVYPLVYDCV
                     APDILLVLLNPLQIPEAPRSAEDIAARTAELGFSTTFLREMRMIAHARRYVAEHQSWR
                     PLGVLEQRLARLRFHLIEAKELAEAGQGSKLNASRSFLHSLRDLGRARARHWLLGHRK
                     DVGIRESVDLAKLFS"
     gene            complement(10228..10632)
                     /locus_tag="PLH85_11360"
     CDS             complement(10228..10632)
                     /locus_tag="PLH85_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65206.1"
                     /translation="MSRASLHTHPCFDTHPGHVRRPEHAIRWQQGLPVEWQPHVVAPL
                     YFDHYMDYELAAARILGRDEDDKACYCAHSFVLDGRGGRHPTTAGAHYAESVRAWRLR
                     DGRWLTHRIVIGGHGALERGRGFFSLGEGMPR"
     gene            11100..13139
                     /locus_tag="PLH85_11365"
     CDS             11100..13139
                     /locus_tag="PLH85_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB65207.1"
                     /translation="MKGKHWQGWMGICLATAGTAVAADGVFQLGTVEVVGSRLESAPP
                     GETAVSQARLTRFNRDTLGEAVTLVPGASLSRNSRNEDIISVRGFDVRQVPLFIDGIP
                     AYVPYDGYVDFGRFSTADVAELRVAKGAASLLYGPNTLGGAINLVTRKPVSALEGDVR
                     AGLGAGNLNTLSANVGSHQGTWYVQAGASYLDRDWFPLSADFAPGSIPTSAANPTRNV
                     LENGGPRENAYQTDSRLSLKLGLTPNASDEYAVGYVSQHGRKGNPPYAGSAPGQVLTL
                     AGGANSRFWQWPYWDLESTYFIANVALGSQYALKTRLYQDNYTNKILAFTNGSYTTQL
                     SNTTNFPSWYDDSTTGLSLELDSFAFTNHELSVAYHLKQDKHVDNGLALSKHYRDVTT
                     SYVVEDAIRLDDAWRLRLGASHDRRDAKEVYYWPTGATSGSNWLAELVRSLGPTAEAY
                     ASVARKTRFPTIKDRYSASLGSGLPNADLKPEHALHLESGVRGRPWSGARGSAAVFVS
                     HIEDLMQRVNILPVTTCSATATACTQLQNIADARHAGVELALDQDLGPAWQVGGQYTY
                     LDRQNRSNPAVPLTDTPRNKLFAYATWKPVARWEVQASADAENGRRVAYGSGNAATYT
                     RLSGYALANLKAVFRPVPAVALEAGASNLFDANYALADGYPMPGRLWFANARYTF"
     gene            13154..14458
                     /locus_tag="PLH85_11370"
     CDS             13154..14458
                     /locus_tag="PLH85_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNB65208.1"
                     /translation="MELLRSPARRAFLRGAGALAAAAGAGPLQAAPRRDVVVLTAYPD
                     AVVSRFEATFEKAHPEYRLRVVWRMPHDALPYLRQAGQGGVDVYWSASPRTYAQLKAE
                     GAFQRAGIDRSGLPGQIGGTQIDDPDGFYLASEVAGYGFACNPAALRARGLGVPQDWT
                     DLADPAYAGQLVMPDPVEVGFAPVMVDIPLQAYGWDTGWALWSAIAANSRFAGRGGSF
                     VADEVGSGRRAVGLSIDFFVAEAIAGGAPLSFAYPRQGGVNPAHIAITAGSPNPAGAR
                     AFMAFVLSEGGQRLLTHPDIRKLPVRPSVYRGLPPTYHNPFAAGAEGGYRYDNARGRG
                     RAPVVAAAFGQALVRPHARLAVLWARLRRAEAPVWNRVWPLLTAPPLSEAQADDEELQ
                     MAFFTRRDDAAAEARAAATEQAWADQVDARLAQAEQAAGLPA"
     gene            14455..15798
                     /locus_tag="PLH85_11375"
     CDS             14455..15798
                     /locus_tag="PLH85_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNB65209.1"
                     /translation="MKVWNGGVAALVLLTLLGAAPRARADAAETADRLFTGREAFSQP
                     LPGLDEAERERFFRGRALFNQSWVVAPARDDRVDGLGPLYNRLACVSCHAHNGRGLPP
                     ERAGERMQAMLVRLSVAGVGPRGGPRPHPAYGDQFNEEGIPGVPGEGRVLLRWQTVRV
                     HGARLRRPVLRFEDLAYGPLGPVLTSPRVGQQVVGMGLIDAVPAATLEALAEAPKPDT
                     VRGRVNRVWNPETGATEVGRFGHKANMPSLRAQIAGAFLGDLGITSPLHPAENCTPVQ
                     TACRRAPSGGAPELSAQQLDEVTYYLAQLAVPPRRGADDPAVRRGEARFAALGCTACH
                     RPMLRTGDSASPALAHQDIAPYTDLLLHDMGPGLADGRPDFAAGGREWRTPPLWGLGL
                     TAAVSERARYLHDGRARSLHEAILWHGGEATVARQRYAALGAAGRAELMAFLGSL"
     gene            complement(15944..16372)
                     /locus_tag="PLH85_11380"
     CDS             complement(15944..16372)
                     /locus_tag="PLH85_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNB65210.1"
                     /translation="MKISARNVFGGTVTAVKPGAVTTEVDVTLPGGIVIVATVTRDSA
                     IELGLIPGRPVKAIIKASSVLVLTEGADVRLSARNCLAGTITALTEGPVSTEVAIGLA
                     DGHTVYATITHEGSVALGLKLGAPATAVFKAPSVILGVST"
     gene            16533..17360
                     /locus_tag="PLH85_11385"
     CDS             16533..17360
                     /locus_tag="PLH85_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNB65211.1"
                     /translation="MTTRRPIHHKLVGKLTVDTEFGTFLGDTRIRLLEAIERYGSISQ
                     AAKAVPLSYKAAWDAVDAMNNLAEEPLVERSVGGKHGGGTSLTEYGQRVIALYRAVEL
                     EYQQAIDRLAKTLGEPGAGSVRQFQTLLRRMSMRTSARNQFVGTVSGLREGEVSFEVR
                     VRLDDAHEIVAVITRESAENLELAIGLEVHAFVKAPSVIIVTDPETRTSARNHLWGEV
                     TRIHPGPVSSEVTITLPSGRNVTSVVTHDSIDMLALAPGVPACAVFQASAVILAVFD"
     gene            17383..18138
                     /gene="modA"
                     /locus_tag="PLH85_11390"
     CDS             17383..18138
                     /gene="modA"
                     /locus_tag="PLH85_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB65212.1"
                     /translation="MKHTFRHASLFLVLAGAALLARAEEISVAVAANFTAPMQKIAAD
                     FEKATGHKAQLVSGSTGKFYAQIKSGAPFQLLLAADDETPARLIKEGDGVSGSAFTYA
                     IGKLVLWSPKPGFVDDKGEVLKAGRFEHLSVANPKLAPYGLAAMEALKALNLADALQP
                     KVVLAESISQAQQFVTSGNAELGFIAYSQIHKDGRIIDGSYWLVPARLYGPIRQDAVI
                     LAQGKGNAAVEALTAYLKSPAAAAVIRSYGYDR"
     gene            18172..18861
                     /gene="modB"
                     /locus_tag="PLH85_11395"
     CDS             18172..18861
                     /gene="modB"
                     /locus_tag="PLH85_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNB65213.1"
                     /translation="MELGPDDLNALLLTAKLATVVTLALLVVGTPIAWWLARTRSRLR
                     GVIGAFVSLPIVLPPTVLGFYLLLLMGPQGLVGQTTRALGLGMLPFSFAGLVVASVFY
                     SLPFVVQPIQNAFLAMGERPLEVAATLRASPWESFFRVALPQARQGFVTAAVLGFAHT
                     VGEFGVVLMIGGNIPGKTQVLSVQIYNHVEALEYGKAHGLAAGLVAFSFLVLLLLQAL
                     GGGRNTTGVGR"
     gene            18858..19934
                     /gene="modC"
                     /locus_tag="PLH85_11400"
     CDS             18858..19934
                     /gene="modC"
                     /locus_tag="PLH85_11400"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNB65214.1"
                     /translation="MSALSLVLRLPLADFELHAELALPGRGVTALFGPSGSGKTTLLR
                     CVAGLARGAGRIVVNDEVWQDDARRIFLPTHRRALGYVFQEASLFEHLTVRGNLEFGL
                     RRILPAERKVSWDQALDLLGIGPLLERRSTTLSGGERQRVAIARALLTSPRLLLMDEP
                     LAALDAPRKQEILPYLERLHDELAIPVLYVSHSADEVARLADHLVLLERGRVVASGAT
                     DALLARLDLPLARDEEAAVRLDTMVLDHDEAYALTTLGFAGGTIQVTRKALPVGARAR
                     LKIQARDVSIALRGDNPSSIVNRVPARVVGFGEAAHPAQCLVALEAGRVRLMARITRL
                     SRDQLGLTAGMEVVAQIKSVALLA"
     gene            20055..>20494
                     /locus_tag="PLH85_11405"
     CDS             20055..>20494
                     /locus_tag="PLH85_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNB65215.1"
                     /translation="MDTPRIALALLAASLALPAAAINKCQTPDGRVTYTDGPCAAGST
                     QTGRVEPLPPVDPADRADALARSKQVVDDARALDAKRDALAAERQKSYDAERRAEEEA
                     RLRQQAADEAATANLYPVPVIVPRVRAPVVPVVKPRPRTEDTDRP"
BASE COUNT         3080 a         6808 c         7268 g         3338 t
ORIGIN      
        1 atgccgcccg aagcccagca ggccgcccaa aacgccgccc gcgcgggcga cgtctgcggc
       61 gccggttgtc gtgcccgccg ctgcagccgc ggcgaccgct acggccgctg ccgcagcgat
      121 gggtccgcgg acggccgagg cgccgcgccc cgatctgccc agacccgaag tcgccgcgcc
      181 gcggcccgag ccggtgcgtc ctgcggtccc gacggccggg gacgctgcgc ctctggcggc
      241 ggcagccccc gccacgcgtc cgagtcgcgg cgtgagccgg gcctacgcga tggatggcgc
      301 gaccttttac cagaacggtc gcaagttccg ggtgcaggga ctcgatgtga acgagcccgg
      361 catgacctcc gaacacgcca cccagcgcct gcaaagggcc ctggactccg gcaatctgac
      421 cgtggatgcg gtggaaagcg atggcgccgg tcacaccgtg gcggtggtcc gcgtgaatgg
      481 tcgcaacgtg gccgatacgg tgcgcgcggc gaactgagct gcacccgatg gccggtggtt
      541 ggcgatctgc cagcccgata gggcgcgtcc gccggtccag gcctgacgcg tgacagggcg
      601 ccaggtcccg gcggacgggc gtgtgacagt gcgcgccttg agtgccgccc gcccccgaac
      661 ccctgcgcgg gtgaccgccg ccacgtgccc ttcatggccg ccaaggcggg aagcgggacg
      721 cgctgtgccg tcacgcgcga acccggtggg tggccaggtg cggggcgcac gtttgccagg
      781 ctgacgtccg gcacgcgaca tggctgcggc tggccggagg ctgacgcccg gccctgctcc
      841 cgggcgatgt cgcggctggg tttgtggggc acacgaccgt cgctgcggac ccacgcaggc
      901 gccggtcggg cgctccgcag gccgcggcgg gtgggcgtcc gcggacgccg ccgcgccgta
      961 cggaccgcga acgtgcgggt ggctcggcct tacagggcga cgagggtctg ccggccccac
     1021 cagcttgcgc cgggggccag ttccacgggc tgccggatcg ccccggcctc gacgcacagc
     1081 atgcgccgga agccgtcggc cggcatgtcg gggagcgcag cgcacttgtg ctcccacgga
     1141 ttccacacca ccacgtcggg gaagttttcg gcgtggatgc ccaggctccg ctgatcctcg
     1201 cggaccagca gggtggcggg ggcgttgtgg tagatgcggt ccacctcgtc ttcgacggtc
     1261 acgctgatgg cggtctcccg cttgagggcg tcaccgtctg cactgtcccg gtactccagt
     1321 ccgcgcagac cttcgatgcg cgtggcttcc acttcccgca cctgcaggta ggtgtgcagg
     1381 gccgcggtga actggaagga ggcgtcgccg gggttgtcca cttccagttc cagatccagg
     1441 cgaccaccgc cgatgcccac gctcagttcg gcgacgaagg catgaggcca cagggcgcgg
     1501 gtcgcgtcgt cgtcggtcag gcgcagggtg accagcacga agtctgggcc ggaacgttgc
     1561 gtcacgagcg tccacggccg gttgcgggcg aagccgtgct tgggcagcgg gccacaggcg
     1621 gcgaactggg ggaagcacac gggtacgcca ccgcgcaggg cggtgcggcc gtcgaacacg
     1681 gctcgagggc tgaggtacag gcgctccgat ccgccggccg gacgccacga caggacctgt
     1741 ccgccgaaca ggctgacgag ggcgacggcg ccgtcggggg tctgcagctt gaggacgggc
     1801 tggccgtgga gttcggcgct ttcgatggct tcggtcatgg cggcggggct gcggattcag
     1861 tcgggccgcg atgatagcgc atgcaccggg gcatggcgga agcatcccgt gaggtgatcg
     1921 ttcaccagcc cggcggactg catgaaggcg tagcagatgg tgctgccgac aaagcggaag
     1981 ccgcgtttct gcagggcttt gctgagggcg tcggagacgg ccgtgcgggc cggcacgtcc
     2041 ttcaggctgg cccaggtgtt gtggatcggt cggccatcga cgaagcgcca cagccaggcg
     2101 tcaaagctgc cgaactcggc ctggaggtcc aggaaggcgc gggcattgcc gatggcggcg
     2161 cggatcttga ggcggttgcg cacgatgccg ggatcggcga ggaggcgggc cgcgtcgtcg
     2221 gcgtcaaagg ccgccacggt ggccgggtcg aagttccgga aggcggcgcg atagccttcc
     2281 cgcttgcgca gaatggtgat ccacgccagc ccggcctggg cgccttcgag gatgaggagt
     2341 tcgaagagcg cccggtcgtc gtggagcggc acgccccatt cccggtcgtg gtaggcctgg
     2401 tacaggggat cttccccgca ccaggcgcag cggacgggcg gggcggcgtc catcagccct
     2461 ggagcagcag gatgccgtgc ttgatggtgt tgtcttccgg gtcgttggac agcacgaagc
     2521 ccaggctctg gacgaacttg agcatgcggt cgttgttgga caggaagatg ccggccatgc
     2581 tgtgcaggcc acggttgcgc gcggtctcga tgagcacgcc catcaggcgg cgggccagac
     2641 cgcggtgctg ccagtcctcg gccacgacga tggcgaactc gcaggactcg ccgtccggat
     2701 tcacggcgta gcgcgccacg ccgatctcca cctcggcgtc gttctcctcg atggtggcga
     2761 cgaaggccat ttcccggtcg tagtcgatct gggtgaggcg ggcgaccatc gcggggggca
     2821 gctcgcgcat ggtgttcatg aagcgcagga acttggtctc gggcgacagc ttgcgcacga
     2881 aatccacttc cagctcggcg tcttcgggct tgatcgggcg gatggtgatc tcgccttccg
     2941 gcatcgacac cttggtgatg aggtgcgacg ggtaggggtg gatcgccatg tggtcgtaac
     3001 ggtcggcggt gggggcgatg ttgtccacca cgatgcgcgc atccacggcc acggcgccgt
     3061 tctcgtccac gatcagcgga ttgatgtcca tctcgcggat ccatggcagc tcgcagacca
     3121 tttcggagat gcgcagcagc acgaactcca gggattccat gttgatgggc ggcatgttgc
     3181 ggtattcgcc cagcagcgtg gacacccggg tggacttgat catgtcctgg gccaggtagc
     3241 tgttcagcgg cggcagggcg acggcgcggt ccttctggat ctccacgtgg gcgccgcctt
     3301 caccgaaggt gatgaccgga ccgaacaccg ggtcgcggaa ggcgccgacc atcagttcgc
     3361 ggccgtaggg tttgatgacc atcggttcga tggcgacgcc gttgatgacg gcgtccgggc
     3421 gtttgcgctt cacgtcgtcc aggatttcct ggtaggcagc acgcacggcg gccagaccgt
     3481 tgaggttgag gcgcacgccg ccggtatcgg ccttgtgggt gatggacggg gaatcgatct
     3541 tcatcgccac gggcagaccg agctcctcgg cgagcaccat cgcttcggtg gcggagcggg
     3601 cgacgaccgt ctgggcgatg ggaatgcgga aggccgccag cagggccttg gactccatct
     3661 cgttgaggtt cttgcggcgc tcggacaggg ccgtttcgat gaccaggcgg gcgctttcca
     3721 ccgacggggg attgaggtcg gaggagaggg acgagggcgt ctgcaccagc agcttctggt
     3781 tgcggtaata ggccgagatg tgcccgaaca gttcgaccgc cggctcggtg gtgcggaagg
     3841 tggggatgcc ggccgcttcg aagagcttgc gcccttcgcg gacctgctct tcgcccatcc
     3901 agcacgtgaa gatcggcttg tccaccgccc gttcgatgtc gatgatcttc tgcgccacct
     3961 cggtggggcg cgccatcgcc tggggcgtga ggatggtcag gacgccatcc acaccttcat
     4021 cggcaagcac cgtctcgagg gtcttgccgt aacgttccgg gtctgcgtcg ccgatgatgt
     4081 ccacagggtt ggtgtgggac cagttctgcg gcaggaactc gttgagcttg gtgatcgtgg
     4141 cgttggacag ggtggcctgg gggatgcgca ggtcggcagc gcggtcggcc gccatgacac
     4201 ccggcccgcc gccattggtg atgatggcga ggcggttgcc gcgaggccgg aaatgggaga
     4261 acagggcgtt ggccgcggcg aagagctggc ccatgttgta caggcggatg acgcccgcgc
     4321 ggcgcagggc ggcgtcgaag accgcgtcct cgccgacggt ggcgcccgag tgcatcagcg
     4381 cggcctggga gccggcgggg tggcggccca ccttgatcag cagcaccggc ttgacgcggg
     4441 ccgcggcgcg cagggcgctg acgaagcggc gggcatcctt gatgccttcc acgtacagga
     4501 agatgctctc ggtgcgggca tccgacacca tgtattcaag gacttcgccg aagtcgatgt
     4561 ccaccgaggt gcccagcgag accaccgacg agaagcccac gttgttgggc cgcgcccagt
     4621 cgaggatggc tgcgcacagg gcgccggact gggagatcag gccgatcgag cctttggtgg
     4681 cgctgccgtg ggcaaagctg gcgttgaggc ccagctcggg gcgcaggatg ccgaggcagt
     4741 tggggccgag caggcggatg cgatgccggc gggcgttttc gacggtgttc ttctcgagca
     4801 tgcggccgcg cgggccggcg tcggagaagc cggaaggcag caccaccacg gacttggtcc
     4861 cggcccgacc gcaggcatcc acgatggccg gcacgcggtc ggccttggtg gtgatcaccg
     4921 ccaggtcgag acgctgcggg atgtcttcga cactcttgta acagggcacg cccagcactt
     4981 cctcgtgctt ggggttcact gcgaagagct tgcccttgaa gccggcgtcc agcatgttgc
     5041 gcacgaggac gttgccgagg gacatctccc gttcgctggc gccgatgatg gcgacggagc
     5101 ggggttcgaa gagcggggtg aggtaatgct gttcattcat gatggatgct cgcgtgagcg
     5161 cggcgaccct gccgcgcggt atgccgttga tacggttttc tcgtcgtctc tgttccgggt
     5221 tgtcagggcg gattctacgc ccgacatgtt gcattgcatc ggacgaacgt ttgaatatcg
     5281 cagcgcacaa ccgccggaca tgactttttt catgaattcc ggggctgagg tgttgcatgg
     5341 cagcaacgtt tccgccagca ggttgacacc gtaaatgtct acggcgtcaa tggcttaggg
     5401 tgaagtagaa actcgcaccc ttttcggggg tggattcggc ccagatggtg ccttcgtggc
     5461 gcaggatgac gcgttgcacg gtggccagac cgatgccggt gccggcgaac tcgttgatgc
     5521 cgtgcatgcg gtggaagggc agaaagagct tcgaggcgaa gttcatgtcg aagcccgcgc
     5581 cgttgtccga gatgcagtag accaggtggc ggtcccgctt ttctgcgtgg aagcggatct
     5641 gggcgtcgtg gcggcgcccg gtgaatttcc acgcgttgcg cagcaggttg tccagcgcga
     5701 cgcggatcag gccccggtcc gcggcggcat agagattggg ctcgatatgc acgtccacga
     5761 tccggtccgg gtcttcgagg cgcagctcgg tgacgatctg ttcggcgatc tggctcagat
     5821 tgacgtgctc ccgcttcatg gtctggcggg tcaggcgggc caggtcgatc aggtcgtcga
     5881 tgaggcaggc catgcgctgg ctggcggcgc ggatgcgttg cagatactcg cggccggtct
     5941 cgtccaggta ggggccgtag tcctccgcga cgatctgggc gaagccgtcg atggcccgca
     6001 gtggcgcgcg cagatcgtgg gagaccgaat aggaaaaggc ctccagctcc cggttggagg
     6061 cctcgagctg ggaggtgcgc acgcgtacgc gggtttccag ttccacattg aggtcgcgct
     6121 gggactgttc ggcgttcttg cggtcggtga tgtcgtccag ggtgccggag gtgccgatgc
     6181 tctggtccag gctgtcgcgc agggggcgga ccgtggcttc gacccagcag gggccggact
     6241 tctgggtgcg caggcggaat tcgaagcggg cgctgtcggc gtagccgccg cggatggaca
     6301 tgagttcgtg ttcgacccgc tggcgatcgt cctcgacgac gaagtcgagc aggctctgac
     6361 tgatggacag gcgggtgtcg aagccggtga tgtggcgcca ggcccggttg aggaaggtga
     6421 gccggtgggc ggcgttggtc tggaagagga tctcattgac cgactcgacc acgtcccggt
     6481 acatggcacg gctttcgcgc agcacctgct cagccgacag ccagcgggcc acgtcccgca
     6541 gcacgaagag cagggcgtcg cggccgtcca ggcgcaggcg gatgcccgag gcctcgatct
     6601 tgagcgggcg gccgtcgagt cgctggtagc gcaccgggag cggcggcagg gcctccagcg
     6661 tcggatcgtc caggaagtgg cgcaggcgct cggcttccat cgtccggtcg tccgggtgga
     6721 tcagctccag cggatccagg ccgtccaggc cgtccatgcc cggcggacag accagacggc
     6781 ggcaggccag gttggcgagg cggatgcgat tgtcctgcac gaccagcatg gcttccggcg
     6841 cctggtcaaa gagctggcgg aagcgcgatt cggcggcctg cagggcgacg agggtggcgt
     6901 gacggtcggt gatgtcctcg atgacggcga cgaaggcgtc gggccgatcc tcggtggccg
     6961 gctgccacgc cagcgtctcg cgacagcgcc gctcggtacc gtctgcgtgg cagaagcggg
     7021 tttcgcgtcc ggcagggggc ggctcgcccg ccaggatgcg cgcgcgcagg gcctggccgt
     7081 cgcgggcatc gtcttccggc tgaaagtcgg cgagagggcg tcccacgagg gtctcggctg
     7141 ccacgcccaa tgtccgtcca agggccgcat tggcggactt ccaggtgcca tccgtgtcga
     7201 tcaccgccag ggcgctcggc gcgtcatcga acacggcacg gtagtgcgtt tcgcgcgact
     7261 gcagggcgag caggcgggcg cgctcggcgg tgatgtcgcg ggtcacgccg caatagccgg
     7321 cgaagaggcc gcgctcgtcg aagaccggca cgccggattc ggacagccag atgtcgtcac
     7381 cggcggcatt gcggcgggaa aagacgaaat tcgagaagga ggcgatctgg cggatctgct
     7441 cgatgtgatc ggcccagggc gcatcggtct gggacacgcc gggcagatcc cagcgccggc
     7501 tgccgagcag gtgttcggcg gccagatcgg cgaggcggcc cgagcgggcg gtgacggcgg
     7561 tgtagcgcag gttggtgtcc tgctcccagt agccgtcggc gctgtgggtc aggactgcgt
     7621 ggaagcgttc ctcggcgcgt tccagggcgc tctcgaggcg cgcacgttcg cttgtctcgc
     7681 ggccgatgcc gcaatagccc cggaaacagc cggtggaatc gaagagcggt cggccgcgca
     7741 gctccaggtg gcggcgctcg ccggtcgggc tggtgagggt gagggccacc gtgatgggac
     7801 cacggcgttc cagggtggtc cggtgggcgg tggcaaagtc cggactgagc tgggggatca
     7861 ccacgtcctc ccagtttcgg ccgatgatgc cgggcggcgc gatgctgggg ctcggtggtg
     7921 cgccggggat gtccacgaag gtgagcgccg catcggtctg ccacagccag tcggaggaca
     7981 gctcgaagaa gtcgcgccag ggcggctgaa ggtgtgggcc ggcaccgtcg gccgttacgg
     8041 tctcggcagg ggtggatccg ccgcggctgc gcatcgcgag gatgccgccc accccggcga
     8101 gcagtccggc gagggccccg gcgacgaagg gcagggcgtc ggccaggacg ttctccatga
     8161 ccgggtcctg cgccgcccgg gcagccagcg gcagggcggt cgccaggcag gcgaacgcga
     8221 ccgggcgtga cgaagccaac gcggtaagga cggggcggaa aaggttcacc agactattat
     8281 cgtcggctgc ggccggtttg ttccgcaccg gaccggggca taaaagcagg agcttagtgc
     8341 gaccctgcaa aattgtgtgt ccatgcgcgt atccccttcg gtattaaatc acaccgcgat
     8401 tcgtgtttga cgagcggggg acagatcggt atgattcgcg cctctcgggg tgtagcgcag
     8461 tccggtagcg cgcttgcttt gggagcaaga tgtcgggggt tcgaatccct ccaccccgac
     8521 caaggctttg cccccgtgac ccgcggcgat ctgcccgtag ctcaattgga tagagcaccg
     8581 gccttctaag ccgggggtta caggttcgat ccctgtcggg caggcttcta caatttggtt
     8641 ccggaatgtc gacgcgagtc ggcagtccct cagtggcggc attagctcag ttggtagagc
     8701 actggattgt gattccaggt gtcaccggtt cgatcccggt atgtcgcccc atgcaacatg
     8761 gcagaaagcc ccgagcaatc ggggctttct gctttccggg gtgtccgcca tcggccctgc
     8821 gtgcccgcgg ctgccggagg ccgtcagggt ggaccgggcg tcgagggtgg gcagcgccgt
     8881 ccacacgcct gtggtcctgc agttcgccgt gatctccgcc gtcacgcgga cgggtgtcgc
     8941 gccggcaccc ggcgcgcgtc cccttggcat gcgacgcatc cggttgtccg cccgggttcg
     9001 cgggcaaggt cgggctgtgc ccgggcgctg ccggacatcc gcacgagaga acttcccccg
     9061 atcgtcccgc tctataaaat ggtgcggcgc acaatccgtg cggcgactca ttcggatgga
     9121 gggcctatgg cgtccagtgt ttggtggaag aaggcattcc ggcgtccgcc ccggctcaat
     9181 cttgccttgc aggggggcgg ggcgcacggc gcctacacgt ggggtgtgct cgatgccctg
     9241 ctggaggcgg gcatcggtct ggaaggcatc agcggcacca gcgccggcgc gatgaatgcg
     9301 gtggcgctgg ccgacggctg gacgcgccac ggcgccgacg gcgcccgcga aagtctggat
     9361 gcgttctggg aggcggtggc ggacagcctg ccgccggaac tggaatggct gcacagcctc
     9421 aatccggcag acgatggcgg tctgcccgga ccgatgcgga tgatgatggg tctgacccag
     9481 tacttctcgc cgtattccct gaatcccctg gaagtgaatc ccctgcggga cgtggtccgc
     9541 gcgcgcttcg acttcgaacg catccgccgg gactgttcat tgaagctgtt catcgcggcg
     9601 acgcgggtac gaacgggcaa ggtgcgcctg ttcaccacgg gcgagctcac ggaagaggcc
     9661 gtgctcgcgt cggcctgcct gccgaccctg caccatgccg tggaaatcga cggcgaggcg
     9721 tactgggatg ggggattcac ggcgaatccg gcggtctatc cgctggtgta cgactgcgtg
     9781 gcgccggaca tcctgctggt gttgctcaat ccgctgcaga ttccggaagc gccccgttcg
     9841 gcggaggaca tcgccgcccg gacagccgag ctgggctttt ccaccacctt tctgcgcgag
     9901 atgcgcatga tcgcccacgc gcgtcgctac gtcgccgagc accagagctg gcggccgctg
     9961 ggggtgctgg agcagcgcct tgcccgcctg cgctttcacc tgatcgaggc gaaggagctg
    10021 gccgaagcgg ggcaggggag caagctcaat gcgtcgcgca gcttcctgca cagtctgcgt
    10081 gatctcgggc gtgcccgcgc ccggcactgg ctgctgggtc accggaagga cgtggggatc
    10141 cgggagagtg tcgatctggc aaaacttttc tcttgaagcg tccccgcggc gagcgctatg
    10201 ccgtccgcag cggacggacc atgccgctca gcgcggcatg ccctccccga ggctgaagaa
    10261 gccccgccct ctttccagcg ccccgtggcc gccgatgaca atccggtggg tcagccagcg
    10321 tccgtcccgc aggcgccagg cgcgcaccga ttctgcgtag tgggccccag ccgtggtggg
    10381 gtggcgcccg ccccgcccgt cgaggacgaa gctgtgggcg cagtaacagg ccttgtcgtc
    10441 ctcgtcgcgg ccgaggatgc gggccgcggc cagctcgtaa tccatgtagt gatcgaaata
    10501 gaggggcgcc accacgtggg gctgccattc gacgggcagg ccctgctgcc agcggatggc
    10561 gtgctcgggt cggcgcacgt ggccggggtg ggtgtcgaag caggggtggg tgtggagact
    10621 tgcgcgggac atggtcaggt cgctttctgc gttgaactcg ggtgggggtg cgacggtggc
    10681 cggaaacccg tgtgcgtcat cccgcgagcg tgctccggct gtgccgggtc atgagcaatt
    10741 tgaatgcccg gattcggcgt cgtggaacgt ttccgttcca atgatggata tgtcgcggaa
    10801 tatatagcgt taggcctgag cagcccgcct gccggactgg cacggagcag cgaccccgtt
    10861 ggcggtgaaa agtggcggat tcatccgggc cgtctgaatc cggcgcgcgc cgtcgtaaat
    10921 ccctcatcac tgtcgctttg ttgacgacgt tatgtagcaa tgtatacatc cattgctgtg
    10981 aaaggcgctt ttctctgcag atggcagatg gcacggtgat tgcacgacga tagccaggcg
    11041 cggttacgta acgcgttata tccatgtgta tataggttgt cgtgcaactc gggggcatca
    11101 tgaaaggaaa gcattggcag ggctggatgg gcatctgtct ggcgacggcg ggaacggccg
    11161 tggcggcgga cggcgtcttc cagttgggga cggtcgaggt cgtcggtagc cggctcgaat
    11221 cggcgccgcc gggagagacc gcggtgagtc aggcgcgcct gacccgcttc aaccgggaca
    11281 ccctgggcga ggccgtgacc ctggtgcccg gtgccagcct gtcccgcaac agccgcaacg
    11341 aggacatcat ctccgtgcgc ggctttgacg tgcgtcaggt gcccctcttc atcgacggca
    11401 ttcccgccta cgtgccctac gacgggtacg tggatttcgg ccgcttcagc accgccgacg
    11461 tggccgagct gcgcgtggcc aagggcgcgg cgtccctgct gtacggccct aacaccctgg
    11521 gcggtgccat caacctggtg acgcgcaagc ccgtgtcggc cctggaaggg gacgtgcgcg
    11581 ccgggctcgg cgccggcaac ctgaacacgc tgtcggcgaa cgtgggcagc caccagggga
    11641 cctggtatgt gcaggccggc gcgtcctacc tggatcggga ttggtttccc ctgtcggcgg
    11701 attttgcgcc gggttcgatc cccaccagcg ccgcgaatcc cacccgcaac gtcctcgaga
    11761 acggcgggcc gcgggaaaat gcctaccaga ccgacagccg cctgtccctc aagctgggcc
    11821 tgacccccaa cgccagcgac gagtacgccg tcggctacgt cagccagcac ggccgcaaag
    11881 gcaatccgcc ctacgcgggc agcgcgccgg gccaggtcct gaccctcgcc ggcggcgcca
    11941 acagccgctt ctggcagtgg ccctactggg atctggagag cacctatttc atcgccaacg
    12001 tggccctggg cagccaatac gcgttgaaga cgcggctcta ccaggacaac tacaccaaca
    12061 agatcctggc cttcaccaac ggcagctaca cgacgcagct gtccaacacc accaacttcc
    12121 cgagctggta cgacgattcc accaccgggc tgtcgctgga actggacagc ttcgccttca
    12181 ccaaccacga gctgtcggtg gcctaccacc tcaagcagga caagcacgtg gacaatgggc
    12241 tggccctcag caagcactac cgggacgtga ccacgtccta cgtggtggag gacgcgatcc
    12301 gcctggacga cgcatggcgc ctgcgcctgg gcgccagcca cgaccggcgc gacgcaaagg
    12361 aggtgtatta ctggcccacc ggcgccacgt ccggcagcaa ctggctggcg gagctcgtcc
    12421 gcagccttgg ccccacggcc gaggcctacg ccagcgtggc ccgcaagacg cgctttccca
    12481 ccatcaagga ccgctactct gcctccctcg gctcgggcct gcccaatgcc gacctcaagc
    12541 cggagcatgc cctgcatctg gaaagcggcg tgcgtggccg tccgtggtcc ggcgcacggg
    12601 gcagcgccgc cgtcttcgtc agtcacatcg aagacctgat gcagcgggtg aacatcctgc
    12661 cggtgaccac ctgcagcgcc accgccaccg cctgcaccca gctccagaac atcgccgacg
    12721 cccggcatgc cggcgtcgag ctggccctcg accaggatct cggaccagcc tggcaggtgg
    12781 gcggccaata cacttacctg gaccgccaga accgcagcaa tcccgccgtg cccctcaccg
    12841 acacgccgcg caacaagctc ttcgcctacg ccacctggaa gcctgtcgcc cgctgggaag
    12901 tccaggcctc ggccgacgcg gagaacgggc gcagggtcgc ctacggcagc ggcaacgcgg
    12961 ccacttacac acgtctgtcc ggctacgccc tggccaacct caaggccgtc ttcaggccgg
    13021 tgccggcggt cgccctggag gcgggtgcca gcaacctgtt cgacgccaac tacgccctgg
    13081 cggacggcta tcccatgccg ggccgcctgt ggtttgccaa cgcgcgttac accttctgat
    13141 gccggagatc gccatggaac tgctccgttc gcccgcgcgc cgggcctttc tgcgcggtgc
    13201 cggcgccctc gccgcggcgg cgggcgccgg gcccctgcag gccgccccgc ggcgtgatgt
    13261 ggtggtgctc accgcctacc cggacgcggt ggtgtcgcgc ttcgaggcca ccttcgagaa
    13321 agcccatccc gagtaccgcc tgcgtgtcgt ctggcgcatg ccccacgacg cgctgcccta
    13381 tctccgccag gcggggcagg gcggggtgga tgtgtactgg agcgcctcgc cgcgcaccta
    13441 cgcccaactc aaggcggaag gcgccttcca gcgcgccggc atcgaccgca gcggcctgcc
    13501 ggggcagatc ggtggtacgc agatcgacga tcccgacggc ttctacctgg caagcgaagt
    13561 cgcgggctac ggcttcgcct gcaatccggc cgccttgcgg gcgcggggtc tgggcgtgcc
    13621 ccaggactgg accgacctgg ccgatccggc ctacgccggc cagctggtga tgcccgaccc
    13681 cgtggaggtg ggctttgcgc cggtgatggt ggacatcccc ctgcaggcct acggctggga
    13741 tacgggctgg gccctgtgga gcgccattgc ggccaacagc cgcttcgccg ggcgcggcgg
    13801 cagctttgtt gccgacgagg tgggcagtgg acggcgcgcc gtcggcctgt ccatcgactt
    13861 cttcgtggct gaagccatcg ccggcggcgc gccgctgagc tttgcctatc cccgccaggg
    13921 cggcgtcaat ccggcccaca tcgcgatcac cgccggcagc ccgaatccgg ccggcgcccg
    13981 ggccttcatg gccttcgtgc tgtcggaggg cgggcagcgc ctcctcaccc acccggacat
    14041 ccgcaagctg cccgtgcggc cctcggtcta tcgcggcctg ccgccgacgt atcacaaccc
    14101 ctttgccgca ggagccgaag gtggctatcg ctacgacaac gcgcgaggtc gcggacgggc
    14161 gccggtggtg gctgcggcct tcgggcaggc cctggtgcgc ccacacgcgc gtttggcggt
    14221 gctgtgggcc cgcctgcggc gcgccgaggc gcccgtgtgg aaccgggtct ggcccctgct
    14281 gacggcgccg ccgttgagcg aggcgcaggc ggatgacgaa gagcttcaga tggccttttt
    14341 cacccgacgc gacgatgcag cggccgaagc ccgcgccgct gccaccgagc aggcctgggc
    14401 cgaccaggtg gatgcccgtc ttgcccaggc cgagcaggcg gcggggctgc cggcatgaag
    14461 gtctggaatg gcggcgtagc cgcactggtc ctgttgaccc tgctgggcgc ggcgccccgt
    14521 gcgcgggccg acgcagcgga gacggccgac cgtctgttca ccggccggga agccttctcc
    14581 cagcccctgc cggggctcga cgaggccgag cgggagcgct tcttccgcgg ccgcgccctg
    14641 ttcaaccaga gctgggtcgt ggcgccggcc agggacgacc gggtggacgg tctcgggccg
    14701 ctctacaacc gcctcgcctg cgtgtcgtgc cacgcccaca acggccgcgg cctgccgccg
    14761 gagcgggcgg gcgagcggat gcaggcgatg ctggtgcgcc tttcggtggc gggcgtcggg
    14821 ccgcggggcg gtccgcggcc ccatccggcc tacggcgacc agttcaacga ggaaggcatc
    14881 cccggcgtgc ccggcgaggg gcgggtgctg ctgcgctggc agacggtccg cgtccatggc
    14941 gcccgcctgc gccgaccggt gctgcgcttc gaggatctgg cgtatggccc cctcggcccg
    15001 gtgctgacat ccccccgcgt cggccagcag gtggtgggca tgggtctgat cgatgcagtg
    15061 cccgcggcca cgctggaggc gctcgccgag gcgcccaaac cagacaccgt gcgcgggcgg
    15121 gtgaaccggg tgtggaatcc cgaaaccggc gccacggagg tcggtcgctt cggccacaag
    15181 gccaacatgc ccagcctgcg cgcccagatc gccggggcct tcctcggcga cctgggcatc
    15241 acctcaccgc tgcatcctgc cgaaaactgc acgccggtgc agaccgcctg ccgtcgggcg
    15301 ccgtccggcg gcgcgccgga gctctcggcc cagcagctcg acgaggtcac gtattacctc
    15361 gcccagctgg ctgtgccgcc ccgtcgcggg gcggacgatc cggcggtgcg tcgtggcgag
    15421 gcgcgtttcg cggcgctggg ctgcactgcc tgccaccggc cgatgctgcg caccggcgac
    15481 tccgcctcgc ccgcgctggc ccatcaggac attgcgccct acaccgatct gctcctccac
    15541 gacatggggc cggggctcgc cgacgggcgt ccggattttg cagcgggcgg acgggagtgg
    15601 cgcacgccgc ccctgtgggg tctcggtctg accgctgcgg tcagcgagcg ggcgcgctac
    15661 cttcacgacg gccgtgcccg cagcctgcac gaggccatcc tgtggcatgg gggcgaggcc
    15721 accgtggcgc ggcagcgtta cgctgccttg ggtgccgcgg gtcgcgccga gttgatggcc
    15781 tttctgggat cgctgtgagc cggcccggat ggtctccggt acgcaccggt ctggggcact
    15841 atcgtgatat agcgctgcat ataggtgcca cgggggcggt ccgcggcggc ggcgtgactg
    15901 aagctgcccc aggctggaac tggatttgat gcgctgcagt aaatcaggtg ctgacgccga
    15961 ggatcacgga gggcgccttg aacaccgcgg tggctggtgc gcccagcttc agccccaagg
    16021 caacggagcc ctcgtgggtg atggtggcat acaccgtatg accgtctgcc agaccgatag
    16081 ccacctcggt gctcaccggg ccttcggtca gggcggtgat cgtgccggcc agacagttgc
    16141 gcgccgacag ccggacgtcc gcgccttccg tgaggaccag caccgaggac gccttgatga
    16201 tggccttcac cgggcggccc gggatcagcc cgagctcaat ggcgctgtcg cgggtgacgg
    16261 tcgcgacgat cacgatgcca ccgggcaggg tgacatccac ttcggtggtg actgcgccgg
    16321 gtttgacggc ggtgacggtc cctccgaaca cgttgcgggc actgatcttc atgaggcatc
    16381 tcctgatttt ggcaatcttg cactgcagca accttggcca ctctacgccc gtgcacgcgg
    16441 acccggcaag gggctgttca ccgattcgag atggagcgga aattgctaaa gatcgctaag
    16501 atccgttgtg tacgacttta tacagagtac tcatgaccac gcgacgtccg attcaccaca
    16561 aactcgtcgg aaagctgacc gtcgataccg agttcggcac ctttctgggc gacacgcgca
    16621 tccgtctgct ggaagccatc gagcgttacg gctccatctc ccaggcggcg aaggccgtgc
    16681 ccctgtccta caaggccgcg tgggatgcgg tggatgccat gaacaacctg gccgaggaac
    16741 ccctggtgga gcgctcggtg ggcggcaagc acggcggcgg taccagcctt accgagtacg
    16801 gccagcgggt catcgccctg tatcgggcgg tggaactgga ataccagcag gccatcgacc
    16861 gcctcgccaa gaccctgggc gagccgggcg ccggttcggt gcgccagttc cagaccctgc
    16921 tgcggcgcat gtccatgcgc accagcgcgc gcaaccagtt tgtcggcacc gtgtccggcc
    16981 tgcgcgaagg ggaggtgagt ttcgaggtgc gggtgcgcct cgacgacgcc cacgaaattg
    17041 tcgcggtcat cacccgcgag agcgccgaaa acctggagct ggccatcggt ctggaggtgc
    17101 acgccttcgt caaggcgccg tcggtgatca tcgtcaccga tcccgagacc cgcacctccg
    17161 cccgcaatca cctgtggggc gaagtgaccc gcatccatcc gggcccggtc agttcggaag
    17221 tcaccatcac cctgcccagc gggcgcaacg tcacttcggt ggtgacccac gacagcatcg
    17281 acatgctggc cctcgcgccg ggggtgccgg cctgcgccgt gttccaggcg tccgccgtga
    17341 tcctcgccgt tttcgactga tcccattccg aaagggtctc ccatgaagca cacgttccga
    17401 catgcatccc tgttcctcgt cctcgccggc gccgcgctgc tggcccgtgc ggaggagatc
    17461 tccgtcgccg tggcggccaa cttcaccgcg ccgatgcaga agatcgccgc cgattttgag
    17521 aaggccacgg gccacaaggc ccagctggtg tccggctcca ccggcaagtt ctacgcgcag
    17581 atcaaatccg gcgcgccctt ccagctgctc ctcgccgccg acgacgagac gccggcccgc
    17641 ctgatcaagg aaggtgacgg tgtgagcggc agcgccttca cctacgccat cggcaagctg
    17701 gtgctgtggt cgcccaagcc cggcttcgtg gacgacaagg gcgaggtgct caaggccggc
    17761 cgcttcgagc acctgtcggt ggccaatccc aagctggcgc cttacggcct ggcggcgatg
    17821 gaagccctca aggccctcaa cctggccgat gccctgcagc cgaaagtcgt gctcgccgaa
    17881 agcatctccc aggcccagca gtttgtcacc tcgggcaacg ccgagctggg cttcatcgcc
    17941 tattcccaga tccacaagga cggcaggatc atcgacggca gctactggct ggtgcccgcg
    18001 cgcctgtacg gtccgatccg ccaggatgcg gtgatcctcg cccagggcaa aggcaatgcc
    18061 gccgtcgagg cgctgacggc ctacctcaag tcgcccgccg ccgccgcggt gatccgcagc
    18121 tacggctacg accggtaagg cttttgccca accgacctca agggaaatgc cgtggaactc
    18181 ggtcccgacg atctgaatgc cttgctcctg acggcgaagc tggctaccgt cgtcaccctg
    18241 gcgctcctgg tggtgggcac gcccatcgcc tggtggctgg cgcggacccg gtcgcgcctg
    18301 cggggcgtga tcggtgcatt cgtctcgctg cccatcgtgc tgcccccgac ggtcctcggt
    18361 ttttatctgc tgctgctcat ggggccacaa ggcctcgtcg ggcagactac ccgcgccctc
    18421 gggctgggca tgttgccctt cagttttgcc gggctcgtgg tggcttcggt gttctattcc
    18481 ttgcccttcg tcgtccagcc catccagaac gcctttctgg cgatgggcga acggcccctc
    18541 gaagtcgcgg ccacgcttcg cgcgtcgccc tgggaaagct tcttccgggt cgccctgccc
    18601 caggcgcgac agggctttgt cacggccgcc gtcctggggt tcgcccatac cgtcggggag
    18661 ttcggggtgg tcctgatgat cggcggcaac attcccggca agacccaggt cttgtcggtg
    18721 cagatctata accacgtcga agcattggag tacggcaagg cccacgggct ggcggccgga
    18781 ctcgtggcct tctccttcct ggtcctgctg ctgctccagg ccctgggcgg aggccggaac
    18841 acgaccgggg tggggcgatg agcgcgctct cccttgtcct gcggctgccc ctcgccgact
    18901 tcgaactgca tgctgagctg gcgctgcccg gacgcggcgt gaccgccctg ttcggtcctt
    18961 cgggttccgg caagaccacc ctgctgcgct gcgtcgccgg cctggcccgc ggcgccggac
    19021 gtatcgtcgt gaatgacgaa gtctggcagg acgacgcccg ccggatcttc ctgcccaccc
    19081 accgccgcgc tctcggctac gtcttccagg aagccagcct gttcgagcac ctgaccgtgc
    19141 gcggcaacct ggaattcggc ctgcgccgca tcctgcccgc ggagcgcaag gtgagctggg
    19201 accaggcgct cgatctgctg ggcatcggcc cgctcctcga gcggcgcagc accacccttt
    19261 ccggcggtga gcgccagcgg gtcgccatcg cccgggcgct cctcaccagc ccgcgcctgc
    19321 tcctgatgga cgagcccctc gcggcgctgg acgcgccgcg caagcaggag atcctgccct
    19381 acctggaacg cctccacgac gaactggcga ttcccgtgct ctacgtgagc cattccgccg
    19441 acgaggtggc gcgcctggcc gatcacctgg tgctgctgga gcgcggccgc gtggtggcca
    19501 gcggcgccac cgacgccctg ctggcgcgtc tcgacctgcc cctggcgcgg gacgaggagg
    19561 ccgcggtgcg cctcgacacc atggtcctcg accacgatga ggcctacgcg ctcaccaccc
    19621 tgggttttgc cggcggcacc atccaggtca cccgcaaggc gctgccggtg ggcgcccggg
    19681 cgcgcctcaa gatccaggcc cgtgacgtga gcatcgccct gcgcggcgac aacccgtcga
    19741 gcatcgtcaa tcgcgtgccg gcgcgtgtcg tcggtttcgg cgaggcggcc cacccggccc
    19801 agtgtctggt ggccctggag gcgggcaggg tgcgtctgat ggcacgcatc acgcgcctgt
    19861 cgcgggacca gctcggcctc acggcgggca tggaggtggt ggctcagatc aagtccgtcg
    19921 cgctgctggc ctgagccggc gcaggtgccc ctcggcggcc gccaccggcc gccgccggac
    19981 ggtgccccgg cgggaacgaa gttccgccac aatcgctcca tgccctcatg agcccttgcc
    20041 cgcgggagcc cgccatggac acgccgagaa tcgcccttgc cctcctggcc gccagcctgg
    20101 cgctgccggc cgcggccatc aacaaatgcc agacgccgga cgggcgcgtc acctacacgg
    20161 acggtccctg cgcggcgggc tccacccaga ccggccgcgt cgagcccctg ccgccggtcg
    20221 atcctgccga ccgggccgat gccctggccc gctcgaagca ggtcgtggac gacgcccgcg
    20281 ccctcgacgc caagcgcgac gccctcgcag ccgagcggca gaaaagctac gacgccgagc
    20341 gccgcgccga agaagaagcc aggctccgcc agcaagccgc cgacgaagcc gccaccgcca
    20401 acctctaccc ggtgccggtg atcgtcccgc gcgtgcgcgc gccggtcgta ccggtggtca
    20461 agcccagacc caggacggaa gacaccgacc ggcc
//
LOCUS       DAOQMM010000067        20287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_954_length_20287_cov_43.183521, whole genome shotgun sequence.
ACCESSION   DAOQMM010000067 DAOQMM010000000
VERSION     DAOQMM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_20287_cov_43.183521"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLH85_11410"
     CDS             <1..460
                     /locus_tag="PLH85_11410"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB65216.1"
                     /translation="RELRLTRELDAPPALVFRLWTEGEHLARWCAPKGFTIPEGGGDV
                     RPGGAWWSVMVAPDGTVHRLGGNYLQVDPPRRLTFTHAWRDAAGQPGHQTIVTVTLDD
                     LGNGRTRLTLHQARFDSVPARDGHSGGWRECLDRLDALARQLVQQQAQQQ"
     gene            495..1013
                     /locus_tag="PLH85_11415"
     CDS             495..1013
                     /locus_tag="PLH85_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB65217.1"
                     /translation="MTAPPTGDSAEFHIERLIDAPREQVWQAHTQAEHLARWWGPKGL
                     GLRVLSLDLRPGGRFHYAMVAPDGSETYGRFVYREILAPARLVWINSFADAAGEAVRH
                     PMAPDWPLHMRVQVDLSEADGRTRLVLRSRTLDASAAECATFEAGFDSMRGGFGGTYD
                     QLDAHLATFNRS"
     gene            1037..1456
                     /locus_tag="PLH85_11420"
     CDS             1037..1456
                     /locus_tag="PLH85_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB65218.1"
                     /translation="MYANAYLFFEGRCEEALQFYQDALGARVEMLLRNRDNPQPPPPG
                     TMPPGTEDKIMHAALRIGDTLVMASDGYCHGGASFQGFALSVSPDSPAEAERVFAALG
                     AGGTVRMPLGPTFWSPAFGMVHDRFGVLWMVNVMPAG"
     gene            complement(1462..3105)
                     /locus_tag="PLH85_11425"
     CDS             complement(1462..3105)
                     /locus_tag="PLH85_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNB65219.1"
                     /translation="MTTPAPPDSDRFSDLLAGVSLAGLLLPEAVAYSSIANLPPQAGV
                     MALFAGLLAYGALGTSRFAVVSATSSSAAVLAAVTASLAGGDAVLRGALAGGMILLAG
                     VFFVLAGLARMGNASDFISKPVLRGFAFGLALTIALKQLPKMVDVHPAQGDLLHFVPA
                     LFGHVADWNLAGLGLGGASLALLFVLERLRPVPGALVVTALGIVLARTVDLAAWGVDT
                     VGPIRLHLAAPDLPDLSRAAWLRLAEVAVAMVLVLYAESYSAIRNFALRHGDAVDVNR
                     DLLALGVANLASGLVHGLPVGAGYSATSANEAAGARSRLSTWVCAAVALLIVLTLLPL
                     IADTPEPVLAAIVVFAVSHSLDPGVFRPCFRLHRDRIVVVAAVVAVLLLGVLDGLLAA
                     IGVSLMMTLKRLSDPALSELGRLAGGHDFVALKLHPEAEALPGILILRPETPLFFANA
                     GHVVTGIRRTLEGHAGLHALIVSLEESPDLDSTSVEALEELAAATRRAGVGLYFTRLK
                     PAALTVLRRAALPGLPPDALSDLSVDDAVTLALSASLVR"
     gene            complement(3152..5878)
                     /locus_tag="PLH85_11430"
     CDS             complement(3152..5878)
                     /locus_tag="PLH85_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNB65220.1"
                     /translation="MPGFVHSRAVVWGLVLGLLAAFAWGAPVAAAGDEAQADVPARVA
                     VLAFRPKDETRARWMPLIDYLNAAGLGRRFELLVMTDTEIDAAVRARQVDVVLTQPAH
                     YVALSTREGLLSPLATLVENEAGHPLARFGGVIVVRADDARLRSVDDLRGKRLVTAAR
                     SSLGGYLMQAGMLQRRGIRLPQDATVTETGYPHDTALAAVLAERADAAFVRSGVIEAK
                     VRDGSLDGAKLRVLDPVADETYPFQTSTPLYPQWPVAAMPWTDEDLARRMAATMLGLP
                     HGGETANRIGLHGFTIPGDYRPVEDLMRRLRVAPYDQVPEFTLVDVWDHYSVLLVSLL
                     AAGMTVLAAAVLLLVRANGRLRAERLRTDEALQRLTEAEAEQRLILDALGEGVYGIDA
                     DGACSFANPAALKLLGLTEGDLLGRDTHALIHHHYPDGRPYPHAECAAHQTARDGMPR
                     RHDDWFWRSDGSGFPVRMTVTPRLEHDQVRGTVVVFSDISESVRVARELDAHRDRLEE
                     QVRQRTAQLEQARAAAEASNRAKSEFLANMSHEIRTPLNAITGMAYLLRRSLLTPAQA
                     ERLDKIDAAGRHLLDIIDAILDLSKIEAGKFALQDGGVDLVRLTGNVLTMLTDRAQAK
                     GLSLVCEPVPALPPLRGDAARLQQALLNYLANAVKFTERGRIVLRVRVVADEGDHLRI
                     CFEVEDTGIGIAPDVLPALFLPFSQADSSITRQYGGTGLGLTITRKLAELMGGTAGVE
                     STPGRGSTFWFSARLAKAATAVGAACGPSVSGAEAQLRQRHAGRRVLLVEDEPVNREI
                     ASELLQDAGLAVSTAEDGIAALERLAAERWDLILMDMQMPRLDGLETTRRLRARPELV
                     GLPVIALTANAFADDRARCLNAGMDDFVAKPVDPEALYATVLKWLDAPRSGA"
     gene            6057..7271
                     /locus_tag="PLH85_11435"
     CDS             6057..7271
                     /locus_tag="PLH85_11435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNB65221.1"
                     /translation="MDRAGLVCQELVSQQTSLRVAVVTETYPPEINGVAMTTGRMVDG
                     LVAAGHRVDLIRPRQAHEGGVVLDADGTDGVEEMLSRGIPIPRYANLQLGLPARKVLL
                     RRWSVQRPDVVQVVTEGPLGWSALAAARKLHLPVISEFHTNFHRYSGHYGIGWLKRPI
                     AAYLRKFHNRADLTLVPTRALRDELAGHGIPRVDVVSRGVDTLLFDPARRSAELRRSW
                     GVGPDDLVVLYVGRIAPEKNLALLEQAYVALKARVPAARLVMVGSGPAQAGMRGRLPD
                     VIFAGPQVGEQLAAHFASGDLFLFPSLTETYGNVVAEALASGLPVVAYRDAAALELIT
                     PGVHGHLAEPGDERGFVHGAVQLAESPELRARMRLAARERVASLAWRAIVDSLVSLQR
                     QVILSSPLSHRR"
     gene            7276..7800
                     /locus_tag="PLH85_11440"
     CDS             7276..7800
                     /locus_tag="PLH85_11440"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB65222.1"
                     /translation="MFALPARLQLIDDRLAVRSNRLSRYRGLRDFLRVVSRLGDGVAW
                     YTLAAALYGLFGAEALPALTQMLAAGVSGVIVYKLLKNHTLRPRPFEVVSDVVCAGSP
                     LDRFSFPSGHTLHAVSFSLILAHHFPPLAGAVFGFALLVAISRPVLGLHYPSDVLAGG
                     AIGASLASATLTLL"
     gene            8026..9366
                     /gene="guaD"
                     /locus_tag="PLH85_11445"
     CDS             8026..9366
                     /gene="guaD"
                     /locus_tag="PLH85_11445"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNB65223.1"
                     /translation="MPSTDTTAHPASILVRGQILHFLADPGPEDDPAAWEYFDDGALW
                     IVDGRIHAAGPWEALHAALPPARRAAATYYDYRGRLVLPGLVDTHLHYAQAGALGSFG
                     RDLLDWLGGAIFPAEASFADPGVAERTAAFVADRLLAHGTTTASVFATVHAHSVDAFF
                     AAAEARQMRVLCGKVLMDRHCPDALRDTAASGAADSLALIERWHGRGRLRYSLSPRFA
                     PTSTPEQLQRTGELFAAWPDLHLQSHLAESHGERAWIARLFPGCRDDAEVYARYGLLG
                     PRAIYAHALHLDDSQRRALAATGTAVAFCPSANLFLGSGFFDYAASTAAGLRVGLASD
                     VGAGTSFSLIRSLGRAFDVSQATGAPLSALRGWFLATLGGAQALSLDDCIGSFQPGRE
                     ADFVVLDLAATPELAWRMGQARSLTDQLFALMILGDERCVVATHLLGRRRWARP"
     gene            complement(9398..11500)
                     /locus_tag="PLH85_11450"
     CDS             complement(9398..11500)
                     /locus_tag="PLH85_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNB65224.1"
                     /translation="MRANLPVTPDEYHLPPGAAIISRTDVQGTIIDCNEEFVRASGFE
                     RMELIGQPHNMVRHPDMPEEAFRDMWATLKRGRPWSGVVKNRRKNGGFYWVRANVTPL
                     MDGAGYMSVRVAPSRVDVQAAEALYARMRKDRSVRLHEGQVARTGPLLRVTRRLMRPW
                     RRSVGARVMSGNAVLLLLLALTAWWASGVIHSSGVDGDRFGRIVQGKDLLADILPPPA
                     YIIESYLVAREMRDQQGAELDAARSRLLKLKQEYDERHAVWQAADLPPELRKALLEEA
                     DAPVAPFYALATGDYYAALRDGNAARAQEALNTLQGLYGAQRKAIDAVVNGTNRWNDA
                     LVGESRDFVTEARTRLGGATALALAVAVVFSALAIRSVVKPLAAVGRAADEIARGNLL
                     CELPQAGEDEIGILVARLALMRNNLHEIAAALRQEAANLKGSLVQMRESAQVSATRAE
                     EQSSYAGALAAAIEELSVSIDHINDNTIRAHGLSADARDGAVEGGQIMVSVSDEISRM
                     SASTQEVVGTMQALEAFAADITKVVDVVSGIADQTNLLALNAAIEAARAGEAGRGFAV
                     VADEVRKLAERTGNSTREITTMVEKVIDGTSKAVADMDANVGGVASVAERARSAGESV
                     TTISGSSVAVLAAMEEIKLGLGEQSVAAREIASRVEQIASVSEANAATASQMRETSRR
                     MAGLAEELQGLTGRFRIA"
     gene            complement(11721..12197)
                     /locus_tag="PLH85_11455"
     CDS             complement(11721..12197)
                     /locus_tag="PLH85_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB65225.1"
                     /translation="MNVVADQDSPRWDHSLDSRVVPLFLALQGARARSLETMRPVLAR
                     YDLSLAEFDVLATLRNAPPPHELTPSQLQAEVVITSGGLTKVMLHLEGRGLVLRRQAA
                     DDARVKPAALTPAGAAQIEAAMAEVVAATGAWLRAALSDAEMARLQTVLARLAGAG"
     gene            12306..13640
                     /locus_tag="PLH85_11460"
     CDS             12306..13640
                     /locus_tag="PLH85_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNB65226.1"
                     /translation="MKAIQRTLAGFLLLLTAGWALSEPGDLVAIASFFDFRSLFMQWS
                     GVIAIGVMSLAMILSLRPAVLEPYLGGLDKLYRLHKWLGITALGITLAHWLMAKSPKW
                     LIALGWLERRGRRPRPSFPEGSLQGLFAGLHGLAEDVGEWAFYGALVLMLIALIKAFP
                     YRRFVQTHRLLPLAYLALVFHAVILLKFDAWRGPIGPLLVALMALGSVAAVSSLLGWR
                     PGRRPVTGTVTAVAADAIDPVLTVDLRLDPGWPGHTAGQFAFVTFDSREGAHPFTLAS
                     AWRGDGYIQFRIKALGDYTRTLAARLHPGARVSLEGPYGRFTFGGNAARQIWIAGGIG
                     ITPFLARLQALAATPDGRRTDLFHCTARYSADAVRQLRHATDAAGVRLHMLWDERDGR
                     LDAARLAETVPDWRDADIWFCGPAPFGDALRTGLVRLGLPARRFHQELFEMR"
     gene            13967..15253
                     /gene="nhaD"
                     /locus_tag="PLH85_11465"
     CDS             13967..15253
                     /gene="nhaD"
                     /locus_tag="PLH85_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNB65227.1"
                     /translation="MLTVLILIFAVTYLAIALEHPLNLHKSASALAGAGLMWTIYALL
                     GPDAATVAHQLDDTVTATAQIVFFLMGAMAIVEVIDAHHGFDVLTRRIRTTRQTTLLW
                     LVGSATFVLSAVLDNLTTTIVMISLLRTLLARHEDRLVFAGLVVIAANAGGAWSPIGD
                     VTTTMLWIGGQLSAAAIIRSALLPSLVCTLVPLLVLSLALRGRPVVAPDAPDPATAGP
                     GATEFERRFMFALGIGVLVAVPAFKTLTHLPPFLGILFGLGIVWLAGDLIHRDKSAEY
                     RERLTLAHALGRIDLGSVVFFVGILLAVATLEHTHLLSALAAWLDLRVGREDLIVLLI
                     GMASAVVDNVPMVAGAMGMYPLADYPADHFLWRFLAYCAGTGGSVLIIGSAAGVAAMG
                     MAHIPFGWYLRRISGLALLGYLAGAGVFVLQQGVAA"
     gene            complement(15296..16624)
                     /locus_tag="PLH85_11470"
     CDS             complement(15296..16624)
                     /locus_tag="PLH85_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB65228.1"
                     /translation="MRRSMRVGVLGLVLGVGNALAQQPGSLRPQVEAQAQRLEAQVIA
                     WRRDIHQHPELGNREFRTGRLVADHLRALGLEVRAPVAHTGVVGVLRGGLPGPVVALR
                     ADMDALPVTEQSGLPFASTQKARWQGQEVGVAHVCGHDGHTAILMGVAQVLASLRERV
                     PGTVVFLFQPAEEGPPPDEEGGAALMIREGALKDPAPSAIFGLHLNSRTPLGKAGVRG
                     GPFMASGDTFRIVVQGSQTHAALPWLGVDPVVLAAQVVMGLQTIESRQVNVLLEPSVI
                     TVGAINGGNRPNIIPDTVELSGMIRALDETMRGDIRKRLIRTAEKIAESGGGSAAVTI
                     TPNYDVTVNDERLARLTAESLRATLGRDQVFVNPKVTVSEDFSAYQKLVPGVFFFLGV
                     VPPDARPETVFPNHSPRFWIDERVLAIGVRTLTNVALDYLTAAAAPAAGD"
     gene            complement(16791..17264)
                     /locus_tag="PLH85_11475"
     CDS             complement(16791..17264)
                     /locus_tag="PLH85_11475"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB65229.1"
                     /translation="MTSADTTLGQDGAGGAAGLDFSWDVERVDWPALVALFERAKLGR
                     RREPERLETAFRNSALCLFVYAGPVLIGVGRALSDGVSRAALYDVAVLPEYQGRGIGQ
                     AIVRRLLAAANVELTMLYAAPGKEAFYAQFGFRRMRTAMAIMPDEADARARGLIE"
     gene            complement(17276..17749)
                     /locus_tag="PLH85_11480"
     CDS             complement(17276..17749)
                     /locus_tag="PLH85_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB65230.1"
                     /translation="MILSGHGLDEVDRTVLRLLQGDGRMSNARLAETLSLSETPCWRR
                     LRRLEAEGFIEGYQARLSRRKLGYGVLAFVQVTLGDHAGEAPLQFERQVAGLPQILSC
                     HNVTGDCDYLLQVVAEDLDAYGLFVRDRLRTLPGVTAIRSSLSLREVKASSALPV"
     gene            17772..18515
                     /locus_tag="PLH85_11485"
     CDS             17772..18515
                     /locus_tag="PLH85_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNB65231.1"
                     /translation="MKLSEQWLFTKDLAILFPFIGPIMKLPHRPTKPTAVIDTAALPL
                     FLAALAAAYLLPGPDMALIVSTTARHGPRSGLMAAAGLALSRSLHVLLSGFGLAALFA
                     AHPLLFHGVKLLGAAYLLGLAWTLLTAPDTTPGATAAAACASPPGAADALRRGFLTNL
                     LNPKALGFCAILLPQFVAPHQPLPGQFAVLGLILVGLGAAFDAAYICAAQGVARRFAA
                     APRLRGLSRLTCAGVFGLAALRLAFSGTD"
     gene            complement(18557..19363)
                     /locus_tag="PLH85_11490"
     CDS             complement(18557..19363)
                     /locus_tag="PLH85_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB65232.1"
                     /translation="MKVRGSVFEGGTRMDFVVDAHMGLALVVGAGLGFFGGLFGIGGG
                     ILAIPLFVLGFGMEQAQAQGTALVLMVPNLLVGWWRYQQRQPMPWRAQAAIAGSATLV
                     TWGMAHFATQLDPLVLRGVFCVFLLALAIKLWRAAPAAAGVGKPLALRWLPLVGAAGG
                     CSMGLLGIGGGLLATPLLAGWLGQRQAVAQGLSLALVAPSSAVALAAYASAGHVDWSR
                     GLPMAVGGLATVSAGVAWAHRLPERRMRTAFAAMLGVTALWLGVGRWLMG"
     gene            19424..>20287
                     /locus_tag="PLH85_11495"
     CDS             19424..>20287
                     /locus_tag="PLH85_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB65233.1"
                     /translation="MNDLPDLAFFALLARQPTLAAAAQALGVTPSSVSRRLAALEHRL
                     GVRLLHRTTRRISLSAEGSRYLEGGEQILHDLDNLERSLLGSREMPRGLLRINATFGF
                     GRRHLAPALSDFARRYPDVEVILDLTDRPLDLTEHAVDVGIRFGPPPDARIQARRIAP
                     NRRLVCAAPAYLDAHGHPESPRDLPRHRCIVIREATAAYNHWQLTDGHEQIMVKVGGP
                     LSTNFGEIAVEWALAGHGIVLRSEWDVAADLRDGRLQRLLSPWAGTSADIHAVYPPRH
                     HLSAKVKVFVAF"
BASE COUNT         2852 a         8145 c         6416 g         2874 t
ORIGIN      
        1 gcgggaactc cgcctcaccc gcgagctcga cgcgccgccg gccctcgtct tccgcctgtg
       61 gaccgagggt gagcacctcg cccgctggtg cgcgccgaag ggcttcacga tcccggaagg
      121 cggcggcgac gtgcgcccgg gcggtgcctg gtggtcggtg atggtggcgc cggacggcac
      181 cgtgcatcgc ctcggcggca actatctgca ggtcgacccg ccccgccgcc tgaccttcac
      241 ccacgcctgg cgcgatgcgg ccgggcagcc ggggcaccag acgatcgtca cggtgacgct
      301 ggacgacctg ggcaatgggc gcacccgcct gacactgcat caggcccggt tcgattcggt
      361 gcccgcccgc gatggccaca gcggcggctg gcgcgaatgc ctcgaccgcc tcgacgccct
      421 cgcccggcag ctcgtgcaac agcaggcgca gcagcaatga ggcccgcgtc cgcccctgac
      481 ccggagtccc gcccatgacc gccccgccca ccggcgacag cgccgaattt cacatcgaac
      541 gcctgatcga cgccccccgc gagcaggtct ggcaggccca cacccaggcc gaacacctgg
      601 cccgctggtg ggggccgaag gggctgggcc tgcgcgtcct gtccctcgac ctgcgccccg
      661 gcgggcgctt ccactacgcc atggtggcgc ccgacggcag cgagacctac ggtcgcttcg
      721 tctatcggga gatcctggcg ccggcgcgcc tggtgtggat caacagcttt gccgacgccg
      781 ccggcgaggc cgtgcgtcac cccatggcgc ccgactggcc gctgcacatg cgcgtgcagg
      841 tggacctgtc cgaggccgac ggccgcacgc gcctggtgct gcggtcccgc accctcgacg
      901 ccagcgccgc cgagtgcgcc accttcgagg ccggcttcga ctccatgcgc ggcggcttcg
      961 gcggcaccta cgaccagctc gacgcccatc tcgccacctt caaccggtcc tgacggacct
     1021 tttccgggag ccccccatgt acgccaatgc ctacctgttc ttcgaaggcc gctgcgagga
     1081 agccttgcag ttctatcagg acgccctcgg cgcccgcgtc gagatgctcc tgcgcaaccg
     1141 ggacaacccc cagccgcctc cgccgggcac gatgccgccg ggcaccgagg acaagatcat
     1201 gcatgccgcg ctgcgcatcg gcgacaccct ggtgatggcc tccgacggct actgtcacgg
     1261 cggcgcgagc ttccagggct tcgccctgtc ggtgtcgccg gacagcccgg ccgaggccga
     1321 gcgggtcttc gcggccctcg gtgcgggcgg caccgtgcgc atgcccctgg gcccgacctt
     1381 ctggtcgccg gccttcggca tggtccacga ccgtttcggc gtgctctgga tggtgaacgt
     1441 gatgcccgcc ggctgaaggc gtcagcgcac cagcgaggcg gacagggcca gcgtcaccgc
     1501 atcgtccacg ctcaggtcgc tgagggcgtc cggcggcagt ccgggcaggg cggcgcggcg
     1561 cagcaccgtc agcgccgccg gcttgaggcg ggtgaagtac aggcccacgc ccgcgcgccg
     1621 ggtcgccgcg gcgagctcct ccagcgcctc cacgctggtg ctgtccaggt ccggcgactc
     1681 ctccaggctg acgatcagcg cgtggagtcc ggcgtggcct tccagcgtgc ggcggatgcc
     1741 ggtcaccacg tggccggcgt tggcgaagaa gaggggggtc tccggacgca ggatgaggat
     1801 gccgggcagc gcctcggcct cgggatggag cttgagggcg acgaagtcgt gcccgccggc
     1861 caggcgcccg agctccgaca gggccggatc ggacagccgc ttgagggtca tcatcaggct
     1921 caccccgatg gccgccagga ggccgtccag cacccccagc aggagcaccg ccaccaccgc
     1981 cgccaccacc acgatgcggt cccggtgcag ccggaagcag ggccggaaga cgcccggatc
     2041 gagggagtga ctcaccgcga agacgacgat ggcggccagc accggctccg gcgtatcggc
     2101 gatgaggggc agcagggtca gcacgatgag cagggccacc gccgcgcaca cccaggtgga
     2161 caggcggctg cgggcaccgg ccgcctcgtt ggccgaggtt gcggaatagc cggcccccac
     2221 cggcaggccg tggaccagcc cggaggccag gttggcgacg ccgagggcga ggagatcccg
     2281 attgacgtcc accgcgtcgc cgtggcgcag ggcgaagttg cggatcgcgc tgtaggactc
     2341 ggcatagagc accagcacca tggccaccgc cacctcggcg aggcgcagcc atgcggcgcg
     2401 gctcaggtcg ggcagatccg gcgcagccag gtgcagccgg atcgggccga cggtgtccac
     2461 gccccaggcg gcgagatcca cggtgcgggc caggacgatg ccgagcgccg tcaccaccag
     2521 cgcgccgggc accgggcgca ggcgctccag cacgaagagc agggccaggc tcgccccgcc
     2581 caggcccagc ccggccaggt tccagtccgc cacatgcccg aacagggccg gcacgaagtg
     2641 cagcaggtcg ccctgggcgg gatgcacatc caccatcttg ggcagctgct tgagggcgat
     2701 ggtgagggcc aggccgaagg cgaagccgcg cagcaccggc ttggagatga aatccgaggc
     2761 gttgcccatc cgcgccaggc cggcgagcac gaagaacacc ccggcgagga ggatcatgcc
     2821 gcccgccagc gccccccgca gcaccgcgtc gccccccgcc aggctggccg tgaccgccgc
     2881 cagcaccgcc gccgacgagg aggtggcgga caccaccgca aagcggctgg tgcccagggc
     2941 gccgtaggcc agcaggccgg caaagagggc catcaccccg gcctggggcg gcaggttggc
     3001 gatgctggaa taggccaccg cctcgggcag caggaggccg gccagcgaga cgccggcgag
     3061 caggtcgctg aagcggtcgg agtcgggcgg cgcgggcgtg gtcatgatcg gcgggagtcg
     3121 tagcgccggg agtctacccc agcgcgccgc gtcaggcccc cgagcgcggg gcgtccagcc
     3181 acttgagcac cgtcgcgtag agggcttccg gatcgacggg cttggccaca aagtcgtcca
     3241 tgcccgcatt caggcagcgc gcccggtcat cggcaaaggc attggcggtg agggcgatca
     3301 ccggcaggcc gaccagttcc ggccgggcgc gcaggcgccg ggtggtctcg aggccgtcca
     3361 gccggggcat ctgcatgtcc atcaggatca gatcccagcg ctctgccgcg agccgctcga
     3421 gggccgcgat gccgtcttcc gcggtgctca cggcgagccc ggcgtcctga agcagctccg
     3481 aggcgatctc ccggttcacc ggttcgtcct ccaccagcag cacacgtcgc cccgcgtggc
     3541 gctggcgtag ctgcgcctcg gcgccgctca ccgacggacc gcaggcagcg cccactgcgg
     3601 tcgccgcctt ggccaggcgg gccgagaacc aaaaggtgct gccccgcccg ggcgtgctct
     3661 ccacgccggc cgtgccgccc atcagctcgg ccagcttgcg cgtgatggtg aggcccagtc
     3721 cggtgccgcc gtactggcgg gtgatggaac tgtccgcctg gctgaagggc aggaacaacg
     3781 ccggcagcac gtccggggcg atgccgatgc ccgtgtcctc cacctcgaag cagatgcgca
     3841 ggtggtcgcc ctcgtcggcc accacgcgca cgcgcaggac gatgcgtccg cgctcggtga
     3901 acttcacggc gttggcgagg tagttgagca aggcctgctg caggcgcgcc gcatccccgc
     3961 gcaggggcgg aagcgccggc accggctcgc acacgaggct caagcccttg gcctgggccc
     4021 ggtctgtcag catcgtgagc acgttgccgg tgaggcggac cagatccacc ccgccgtcct
     4081 gcagagcgaa cttgccggcc tcgatcttgg acaggtcgag gatggcgtcg atgatgtcga
     4141 gcagatggcg ccccgccgca tcgatcttgt ccaggcgctc cgcctgggcg ggcgtgagca
     4201 gcgagcggcg cagcagatag gccatgccgg tgatggcatt gagcggcgtg cggatctcgt
     4261 gggacatgtt ggcgaggaac tcgctcttgg cccggttcga ggcctctgcg gcggcccgcg
     4321 cctgctccag ctgggcggtg cgctggcgca cctgctcttc caggcggtcg cggtgggcat
     4381 ccagttcgcg ggcgacgcgc acgctttcgc tgatgtccga aaacaccacc accgtcccgc
     4441 gcacctggtc gtgctcaagg cgcggcgtga ccgtcatgcg caccggaaag ccactgccgt
     4501 cggatcgcca gaaccagtcg tcgtgtcgtc gcggcatgcc gtcgcgggcg gtctggtggg
     4561 ccgcgcattc ggcgtgggga taggggcggc cgtccgggta atggtggtgg atcagggcgt
     4621 gggtgtcccg ccccagaagg tccccctccg tgaggcccag cagcttcagg gcggccgggt
     4681 tcgcgaagga gcacgcaccg tcggcgtcga tgccgtacac cccctccccc agggcatcga
     4741 ggatcaggcg ctgttcggcc tcggcctcgg tgaggcgttg cagcgcctcg tcggtacgca
     4801 ggcgctcggc ccgcaggcgg ccgttggcgc ggaccagcag cagaaccgcc gccgccagca
     4861 ccgtcatgcc cgccgcaagc agggatacaa ggagcacgct gtagtggtcc cacacatcca
     4921 ccagggtgaa ttcgggcacc tgatcgtagg gcgccacccg cagccggcgc atcagatcct
     4981 ccaccggccg gtaatcgccg gggatggtga agccgtgcag gccgatgcgg ttcgccgtct
     5041 cgccaccatg gggcagcccg agcatcgtcg cggccatgcg ccgggccaga tcctcgtcgg
     5101 tccacggcat ggccgccacc ggccattggg gatagagggg ggtagaggtc tggaagggat
     5161 aggtctcgtc cgccacaggg tcgaggacgc gcaacttcgc gccatccagg gagccgtcgc
     5221 ggaccttggc ctcgatcacg ccgctccgca cgaaggccgc atcggcgcgt tcggccagca
     5281 ctgccgcgag ggccgtatcg tgggggtagc cggtctcggt caccgtcgcg tcctggggca
     5341 ggcggatgcc gcgccgctgg agcatgccgg cctgcatcag gtagccgccc agggagctgc
     5401 gtgccgccgt gaccagccgc ttgccgcgca gatcgtccac gctgcgcagc cgcgcatcgt
     5461 cggcgcggac cacgatcacc ccgccgaagc gggcgagcgg atggccggcc tcgttctcca
     5521 ccagggtcgc caggggcgac agcagacctt cccgcgtgct cagggccacg tagtgggcgg
     5581 gctgggtcag caccacatcc acctgccgcg cccgcacggc ggcgtcaatc tcggtgtcgg
     5641 tcatcaccag cagctcgaag cgccgcccga gcccggcggc gttgaggtaa tcgatcaggg
     5701 gcatccagcg cgcccgcgtc tcgtccttgg ggcggaaggc cagcacggcc acccgcgccg
     5761 gcacgtcggc ctgtgcctcg tccccggcgg cggccaccgg cgcaccccac gcgaacgcag
     5821 ccagcagccc cagcaccagc ccccacacga cagcccgcga atggacgaaa cccggcacct
     5881 tcccctcccc gccgcccgcc ggcagcctca aaaacccatg acccacatag ggatatatcg
     5941 gcacgccggc agaaaactca acgcccccgc cctgtcacac gaacacaccg ggaccgtcac
     6001 caagggatca cctgtccgtc acaccgttgc agcatgccgc cgcaacactg gcggccatgg
     6061 atcgcgccgg actcgtttgc caggaactcg tcagccaaca gacctccctc cgggtggctg
     6121 tggtcacgga gacctatccg cccgaaatca acggggtggc catgaccacc ggccggatgg
     6181 tggatgggct cgtcgccgcc ggtcaccggg tggatctgat ccgcccgcgc caggcccacg
     6241 aaggcggcgt cgtgctcgac gcggatggca ccgacggcgt cgaggagatg ctctcccgcg
     6301 gcattcccat cccccgctat gccaacctgc aactgggcct gccggcgcgc aaggtgctgc
     6361 tgcggcgctg gtccgtgcag cgcccggacg tggtccaggt ggtcaccgag ggccccctcg
     6421 gctggtcggc cctggccgcc gcgcgcaagc tgcacctgcc ggtgatcagc gaattccaca
     6481 ccaacttcca ccgctacagc ggccactacg gcatcggctg gctcaagcgc cccatcgccg
     6541 cctacctgcg caagttccac aaccgcgccg acctcaccct ggtgcccacc cgcgccctgc
     6601 gtgacgaact ggccggccac ggcatcccgc gggtggacgt ggtctcccgc ggcgtggaca
     6661 ccctcctctt cgacccggcc cggcgcagcg ccgagctgcg ccgcagctgg ggcgtcggtc
     6721 cggacgatct ggtggtgctc tacgtgggcc gcatcgcccc cgaaaagaat ctcgccctgc
     6781 tggagcaggc ctacgtcgcc ctcaaggccc gcgtacccgc cgcccgcctc gtgatggtgg
     6841 gcagcggccc ggcccaggcg ggcatgcgcg ggcgcctgcc cgacgtgatc ttcgccggcc
     6901 cccaggtggg cgagcagctc gccgcccact tcgccagcgg tgacctgttc ctcttcccga
     6961 gcctcaccga gacctacggc aacgtggtcg ccgaagccct cgccagcggc ctgccggtgg
     7021 tggcctaccg ggacgccgcg gccctcgagc tgatcacccc cggcgtgcat ggccatctgg
     7081 ccgaacccgg tgacgagcgc ggcttcgtgc acggcgccgt ccagctcgcc gaatcgcccg
     7141 aactgcgcgc ccgcatgcgc ctggccgccc gcgagcgggt cgccagcctg gcctggcggg
     7201 ccatcgtgga cagcctggtc agcctgcagc ggcaggtgat cctctcctcg cccctctccc
     7261 accggaggtg atgccatgtt cgccctgccc gcccgcctgc aactgatcga cgaccgcctc
     7321 gcggtccgct ccaaccgcct gagccgttac cgcggcctgc gggattttct gcgcgtggtg
     7381 agccgcctcg gcgacggcgt ggcctggtac accctggccg ccgccctcta cggcctgttc
     7441 ggcgccgagg ccctgccggc cctcacccag atgctggccg ccggcgtcag cggcgtcatc
     7501 gtctacaagc tgctcaagaa ccacaccctg cgcccgcgcc ccttcgaggt ggtgagcgac
     7561 gtggtgtgcg ccggctcgcc gctggaccgc ttcagcttcc cctccggcca caccctgcac
     7621 gcggtgagct tctccctgat cctcgcccac cacttcccgc ccctggccgg cgcagtcttc
     7681 ggctttgccc tgctggtggc gatctcccgc ccggtgctgg gcctgcacta tcccagcgac
     7741 gtgctggccg gcggcgcgat cggcgccagc ctcgccagcg ccaccctgac cctgctgtaa
     7801 atcccggccc gggcggcgga cctgcgccac gcgcggggcg cggcccccgc gccggccgca
     7861 ttgggtccca cgcgacctgt cggtcctcgg cccgcgccca tgcggatcac ccgccgcggg
     7921 cgtcgcgatg tccgtggccg tcatcgtgcc tctcccgccc ccggccgcgc cctgcaaccc
     7981 ggcgcccatt gctatcctga aggggacgac cgcccagccc ccgccatgcc ctccaccgac
     8041 accaccgccc acccagcgtc catcctggtc cgcggccaga tcctgcactt cctcgccgat
     8101 cccggcccgg aagatgaccc cgccgcctgg gaatacttcg acgacggcgc cctgtggatc
     8161 gtggatggcc gcatccacgc cgccggcccg tgggaagccc tccacgccgc cctgccaccg
     8221 gcccgccgcg ccgccgccac ctactacgac taccgcggcc gcctggtcct gcccggcctg
     8281 gtcgacaccc acctgcacta cgcccaggcc ggcgccctcg gcagcttcgg gcgcgacctg
     8341 ctggactggc tcggcggcgc catcttcccg gcggaggcca gcttcgccga ccccggcgtc
     8401 gccgagcgca ccgccgcctt cgtcgccgac cgcctgctcg cccacggcac caccaccgcc
     8461 tcggtcttcg ccaccgtcca cgcccactcg gtggatgcct tcttcgccgc cgccgaagcc
     8521 cgccagatgc gcgtgctgtg cggcaaggtg ctgatggacc ggcactgccc cgacgccctg
     8581 cgcgacacgg ccgcctccgg cgctgccgac agcctcgccc tcatcgaacg ctggcatggc
     8641 cgcggacgcc tgcgctacag cctgagccca cgcttcgccc ccacctccac gccggaacag
     8701 ctgcagcgca ccggcgaact cttcgccgcc tggcccgacc tgcacctgca gagccacctg
     8761 gcggaaagcc acggcgagcg tgcctggatc gcccgcctct tccccggctg tcgcgacgat
     8821 gccgaggtct acgcccgcta cggtctgctc ggcccgcgcg ccatctacgc ccacgccctc
     8881 cacctggacg attcccagcg ccgcgccctg gccgccaccg gcacggccgt cgcgttctgt
     8941 cccagcgcca acctcttcct gggcagcggc ttcttcgact acgccgccag cacggccgcc
     9001 ggcctgcgcg tcggcctcgc cagcgacgtg ggcgccggca ccagcttcag cctgatccgc
     9061 agcctcggcc gcgccttcga tgtgagccag gccaccggcg cgcccctgtc ggccctgcgc
     9121 ggctggtttc tcgccaccct gggcggcgcg caggcgctct ccctggacga ctgcatcggc
     9181 agcttccagc ccggccgcga ggccgatttc gtggtactcg atctggccgc cacaccggag
     9241 ctggcgtggc gcatgggaca ggcgcgcagc ctcacggacc agctgttcgc gctgatgatc
     9301 ctgggtgacg agcgctgcgt cgtcgccacc catctgctcg gccgccggcg ctgggcgcgg
     9361 ccctgaacga cccgccgcgg cgggtgccgg gcagccgtca ggcgatgcgg aagcgtccgg
     9421 tcaggccctg cagctcctcg gccagacccg ccatgcggcg cgaggtctcg cgcatctgcg
     9481 aagccgtcgc ggcattggcc tccgacacgc tggcgatctg ctccacgcgc gaggcgatct
     9541 cccgcgcggc cacggactgc tcgcccaggc cgagcttgat ctcctccatc gccgccagca
     9601 ccgccaccga gctgcccgag atcgtcgtca ccgactcccc ggcggagcgg gcccgctcgg
     9661 ccacgctggc gaccccgccc acattcgcgt ccatgtccgc cacggccttc gacgtcccgt
     9721 cgatgacctt ctccaccatc gtggtgatct cccgcgtcga gtttccggtg cgctccgcca
     9781 gcttgcgcac ctcgtcggcc accaccgcaa agccgcgccc ggcctccccg gcgcgggccg
     9841 cctcgatggc ggcgttgagg gccagcagat tggtctggtc ggcgatgccg ctcaccacat
     9901 ccaccacctt ggtgatgtcc gcggcaaacg cttccagcgc ctgcatggtg cccaccacct
     9961 cctgcgtgga cgccgacatg cgcgagatct cgtccgacac gctcaccatg atctgcccgc
    10021 cctccacggc cccgtcgcgg gcgtcggcgg acaggccgtg ggcgcggatg gtgttgtcgt
    10081 tgatgtggtc gatggacacc gacagctcct cgatcgccgc cgccagggcg ccggcgtagg
    10141 acgactgctc ctccgcccgc gtggcgctca cctgggccga ctcgcgcatc tgcaccagac
    10201 tccccttcag attggccgct tcctggcgca aggctgcggc aatctcgtgc aggttgttgc
    10261 gcatcagcgc caggcgcgcc acgaggatgc cgatctcgtc ttctccagcc tggggcagct
    10321 cgcacagcag attgccgcgg gcgatctcat ccgcggcgcg ccccactgcc gccaggggct
    10381 tcaccaccga gcggatcgcc agggcggaaa agaccaccgc caccgccagc gccagcgccg
    10441 tcgcaccgcc caggcgggtg cgcgcctccg tcacgaaatc gcgggactcg ccaaccagcg
    10501 cgtcattcca gcggttggtc ccgttcacca ccgcgtcgat ggccttgcgc tgggcaccgt
    10561 agagtccctg caaggtgttc agggcctcct gcgcgcgggc cgcgttgccg tcccgcagcg
    10621 cggcgtagta atccccggtg gcgagggcat agaacggcgc caccggcgcg tcggcctcct
    10681 cgaggagcgc cttgcgcagc tcgggcggca ggtccgccgc ctgccacacg gcgtgacgtt
    10741 cgtcgtattc ctgcttcagc ttgagcagac ggctccgcgc ggcgtccagt tcggcgccct
    10801 gctgatcgcg catctcccgc gccaccagat agctttcaat gatgtaggcc ggcggcggca
    10861 ggatgtcggc gagcaggtcc ttgccctgca cgatgcgccc gaaacggtcg ccatccacgc
    10921 cgctggaatg gatgacaccg ctcgcccacc aggcggtcag ggccagcagc aggagcagca
    10981 ccgcgttgcc gctcatgacc cgcgccccca ccgagcggcg ccagggccgc atcagccggc
    11041 gtgtcacgcg cagcagcgga ccggtccggg cgacctgccc ttcatgcaac cggacgctcc
    11101 gatccttgcg catgcgcgcg tagagcgcct cggccgcctg cacgtcgacc cgcgacggcg
    11161 ccacccgcac cgacatgtag cccgccccgt ccatcagcgg cgtgacgttg gcgcgtaccc
    11221 agtagaaacc accgttcttg cgccggttct tgacgacgcc cgaccacgga cggccgcgct
    11281 tcagcgtcgc ccacatgtca cggaaggcct cctccggcat gtccggatgg cggaccatgt
    11341 tgtggggctg accgatcagc tccatgcgtt cgaagccgct cgcccgcacg aactcctcgt
    11401 tgcaatcgat gatggtgccc tgaacgtcgg tgcggctgat gatcgccgcg cccggcggca
    11461 ggtgatattc gtccggcgtg accggcaggt tcgcgcgcat gatcgtgtcc gtcctctcgc
    11521 gtttcggttt cggtgaaccg tcttccggac cgggtccggc cgcctccgtc gcgcctgcgc
    11581 tgcgcgcacg ccgcaccctt tcctgtccga catcaatgac aactgatgcg ctatcgacgt
    11641 tggaaacgcc gccttgagag cacgttttgc atttttttca tttcggaagg cagcactgtc
    11701 aggcccaccc ccgatgcgcg tcagcccgcc cccgccagcc tcgccagcac ggtctgcagg
    11761 cgggccatct ccgcgtccga cagcgccgcg cgcagccacg cgcccgtcgc cgccaccacc
    11821 tcggccatcg ccgcctcgat ctgcgccgcg cccgccggcg tcagcgctgc cggcttgacc
    11881 cgcgcgtcgt ctgccgcctg gcggcgcagc accagcccgc gcccctccag gtgcagcatc
    11941 accttggtga gcccgccgga ggtgatcacc acctctgcct gcagttggga gggcgtcagc
    12001 tcgtggggcg gtggggcatt gcgcaaagtc gccagcacgt cgaactccgc cagggacaag
    12061 tcgtagcggg ccaggaccgg gcgcatcgtc tccagcgagc gggcgcgcgc gccctgcagg
    12121 gccaggaaga ggggcacgac ccgggaatcg agactgtgat cccagcgcgg agaatcctga
    12181 tcggcaacga cgttcatggg tgtcctcggg aaacatcccc gttatgtaaa ggcaggcaaa
    12241 tcatcttgct ggaaagatat ctgcccgata gattggactc aagtcccatt cagatccaat
    12301 ccaccatgaa agccatccag cgcaccctcg ccggcttcct gctcctgctc accgccggct
    12361 gggccctctc cgagcccggc gacctcgtgg ccatcgccag cttcttcgac ttccgcagcc
    12421 tcttcatgca atggagcggc gtgatcgcca tcggcgtcat gagcctcgcc atgatcctgt
    12481 ccctgcgccc cgccgtcctc gaaccctacc tcggcgggct ggacaagctc taccgcctgc
    12541 acaaatggct gggcatcacc gccctgggca tcaccctcgc ccactggctg atggccaagt
    12601 cccccaagtg gctgatcgcc ctcggctggc tcgaacgccg cggccgccgc ccgcgcccga
    12661 gctttcccga aggctccctg cagggcctct tcgccggcct ccacggcctc gccgaagacg
    12721 tgggcgaatg ggccttctac ggcgccctgg tgctgatgct catcgccctc atcaaggcct
    12781 ttccctaccg ccgcttcgtg cagacccacc gcctgctgcc cctcgcctat ctggcgctgg
    12841 tcttccacgc cgtcatcctg ctcaagttcg acgcctggcg cggccccatc ggccccctgc
    12901 tggtcgcgtt gatggccctc ggcagcgtcg ccgccgtgtc cagcctgctc ggctggcgcc
    12961 ccggcaggcg ccctgtcacc ggcaccgtca cggccgtggc cgccgacgcc atcgatccgg
    13021 tcctcaccgt cgacctccgg ctcgaccccg gctggcccgg ccacaccgcc ggccagttcg
    13081 ccttcgtcac cttcgacagc cgcgagggcg cccacccctt caccctcgcc tccgcctggc
    13141 gcggcgacgg ctacatccag ttccgcatca aggccctcgg cgactacacc cgcacgctgg
    13201 ccgcccgtct ccaccccggc gcccgcgtca gcctggaagg cccctacggc cgcttcacct
    13261 tcggcggcaa cgccgcgcgt cagatctgga tcgccggcgg catcggcatc acccccttcc
    13321 tggcccgcct gcaggccctc gccgccaccc ccgacgggcg ccgcacggat ctcttccact
    13381 gcaccgcgcg ctattccgcc gacgcggtcc gtcagctccg ccacgccacc gacgccgccg
    13441 gcgtgcgcct gcacatgctg tgggacgaac gggacggccg cctcgacgcc gcccgcctcg
    13501 ccgagaccgt gcccgactgg cgcgacgccg acatctggtt ctgcggtccg gcgcccttcg
    13561 gcgacgccct gcgcacgggc ctcgtccgcc tcggcctgcc cgcccggcgc ttccaccagg
    13621 aactcttcga gatgcgctga gcacgcggcg cggcgcgcgc gtcccctgcc gcgccagggc
    13681 ccggacgccc gcccgctgcg ctagaatcca ccccgggtgt ccctccgcgc cgtgcggacg
    13741 gacaggtttc gtgacggtcg ggccgcctca cgggaacatc ccatcatggc cctccgccca
    13801 ctgcgcagac gcctgcgcgc ccgcctgtcc cccgccacga cggcaccgcc gtgtcccgcc
    13861 gttgctgcct tcgcggcccc ccacgcacgc ttccccgcac cgccgcgacg ccctgacgcg
    13921 ccgcgctgcg ccattcggcg cctccccgtc tgaccggaat gcccccatgc tcaccgtcct
    13981 catcctgatc ttcgcggtca cctacctggc catcgccctc gagcaccccc tcaacctcca
    14041 caaatccgcc tcagccctgg ccggcgccgg tctgatgtgg accatctacg ccctgctcgg
    14101 gccggacgcc gccaccgtcg cccaccagct cgacgacacc gtcaccgcca cggcgcagat
    14161 cgtcttcttc ctgatgggcg ccatggccat cgtcgaggtc atcgacgccc accacggctt
    14221 cgacgtcctc acccgccgca tccgcaccac ccggcagacc accctgctgt ggctcgtcgg
    14281 cagcgccacc ttcgtgctga gcgccgtgct cgacaacctc accaccacca tcgtgatgat
    14341 ctccctgctg cgcacgctgc tggcccgcca cgaggaccgc ctcgtctttg ccggcctcgt
    14401 ggtcatcgcc gccaatgccg gcggggcgtg gtcccccatc ggcgacgtca ccaccaccat
    14461 gctgtggatc ggcggccagc tcagcgccgc cgccatcatc cgctcggccc tgctgccgtc
    14521 cctcgtgtgc acgctggtgc ccctcctggt cctcagcctc gccctgcgcg gccggcccgt
    14581 cgtcgcgccg gacgcgccgg accccgccac cgccgggccc ggcgcaaccg agtttgagcg
    14641 ccgcttcatg ttcgccctgg gcatcggcgt gctcgtcgcg gtccccgcct tcaagaccct
    14701 gacccacctc ccgccctttc taggcatcct cttcgggctg ggcatcgtgt ggctggcggg
    14761 cgacctgatc caccgggaca agtccgccga gtaccgcgaa cgcctgaccc tcgcccacgc
    14821 cctcggccgc atcgacctgg gctccgtcgt gttcttcgtc ggcatcctgc tggccgtcgc
    14881 cacgctggaa cacacccacc tgctgtccgc cctcgccgcc tggctggacc tgcgggtggg
    14941 ccgcgaagac ctcatcgtgc tgctcatcgg catggccagc gcggtggtgg acaatgtgcc
    15001 catggtggcc ggcgcgatgg gcatgtatcc cctcgccgac taccctgcgg atcacttcct
    15061 gtggcgcttc ctggcctatt gcgcaggcac cggcggctcc gtgctgatca tcggctcggc
    15121 ggcgggcgtc gccgccatgg gcatggccca catccccttc ggctggtacc tgcggcgcat
    15181 cagcgggctc gccctgctcg gctacctggc gggcgccggg gtgttcgtgc tgcagcaggg
    15241 cgtggcggcc tgacccacgc cggtccacgg ccgcccggcg cgcgccggca cggcgtcaat
    15301 cgccggccgc cggtgcggcg gcagccgtga ggtagtccag ggccacgttg gtcagggtgc
    15361 gcacgccgat ggcgagcacc cgctcgtcga tccagaagcg cggcgaatga ttggggaaca
    15421 ccgtctccgg ccgcgcgtcc ggcggcacca ccccgaggaa gaagaagacc cccggcacca
    15481 gcttctggta cgcggagaaa tcctccgaaa cggtgacctt gggattcacg aacacctgat
    15541 cgcgccccag cgtggcccgc agggactccg ccgtcaggcg ggccagacgc tcatcgttca
    15601 ccgtcacgtc gtagttcggc gtgatcgtca ccgccgcgct gccgccgccg ctctccgcga
    15661 tcttctcggc cgtgcggatc aggcgcttcc ggatgtcgcc gcgcatggtc tcgtcaaggg
    15721 cgcggatcat gccgctcagc tccaccgtgt cggggatgat gttcggccgg ttgcctccgt
    15781 tgatcgcgcc gacggtgatc accgacggtt cgagcagcac attcacctgg cggctctcga
    15841 tggtctgcag gcccatgacc acctgcgccg ccagcaccac gggatcgacg cccagccacg
    15901 gcagggccgc gtgggtctgg ctgccctgca ccacgatccg gaaagtatcg ccgctcgcca
    15961 tgaaaggccc gccgcgcacg cccgccttgc ccaggggcgt ccggctgttg aggtgcaggc
    16021 cgaagatcgc cgagggcgcc ggatccttca acgccccttc gcggatcatc agcgcggccc
    16081 cgccctcctc gtcgggcggc gggccttcct cggcgggctg gaagaggaac accaccgtgc
    16141 ccggcacccg ctcccgcagc gacgcgagca cctgcgccac gcccatcagg atcgccgtat
    16201 ggccgtcgtg accgcacaca tgggcgacgc ccacctcctg cccctgccag cgtgccttct
    16261 gcgtcgacgc aaaaggcagg ccgctctgct cggtgaccgg cagggcgtcc atgtcggccc
    16321 gcagcgccac caccggcccc ggcagaccgc cgcgcaacac ccccacaacg ccggtgtggg
    16381 ccaccggcgc acgcacctcc agccccagcg cgcgcagatg atcggccacc agcctgcccg
    16441 tccggaactc ccgattgccc agctcgggat gctgatggat gtcccgtcgc cacgcgatca
    16501 cctgcgcctc cagacgctgc gcctgcgcct ccacctgcgg ccgcaggctt cccggctgct
    16561 gcgcgagggc attgcccacc cccaggacaa ggcccagcac ccccacgcgc atgctccgtc
    16621 gcatcgtcgc cgctcccgaa tggatccgcc cgccccgcgc tgcgccgccg cagcccctct
    16681 ggatcattct agacaggcac cgccatttcg ccggacgcct gcgccggcgg cccccggccc
    16741 gcgacgcagt ctgcgcgccc cacgggtcgc ggccgcactg tgcgcggcgc ctactcgatc
    16801 agcccccgcg cccgcgcatc cgcctcgtcc ggcatgatcg ccatcgccgt gcgcatgcgc
    16861 cggaaaccga actgcgcgta gaacgcctcc ttgcccggcg cggcgtagag catcgtcagc
    16921 tccacattgg cggcggccag cagacgccgc acgatggcct gcccgatgcc gcgcccctgg
    16981 tattcgggca acaccgccac gtcgtacagc gccgcccgcg acaccccgtc cgacaaggcc
    17041 cgccccacgc cgatcagcac cggcccggca tacacgaaga ggcacagcgc gctgttgcgg
    17101 aaggccgtct ccaggcgctc gggctcacgc cgccggccca gcttcgcccg ctcgaagagc
    17161 gccaccagcg ccggccagtc cacccgctcg acgtcccacg aaaagtccag ccccgcggcc
    17221 ccgccagcgc cgtcctgccc cagcgtcgtg tctgcggatg tcatgtgtcc cgccctcaca
    17281 ctggcagcgc cgacgacgcc ttcacttccc gcagggacag gctcgaacgg atcgccgtca
    17341 cccccggcag cgtgcgcagg cggtcgcgca cgaagagccc gtaggcgtcc agatcctccg
    17401 ccaccacctg caacaggtaa tcgcagtcgc ccgtcacgtt gtgacaggac aggatctgcg
    17461 gcagcccggc cacctgtcgc tcgaactgca ggggcgcctc cccggcgtga tcccccagcg
    17521 tcacctgcac gaaggccagc accccgtagc ccagcttgcg ccggctgagc cgcgcctgat
    17581 agccctcgat gaagccctcc gcctccagcc gacgcaggcg tcgccagcac ggcgtctcgc
    17641 tcagcgacag ggtctcggcc agacgcgcgt tggacatgcg cccgtccccc tggagcaggc
    17701 gcaacaccgt gcgatccacc tcatccagcc catgtccgga aagaatcatt ccctgcctcc
    17761 tcgatatcag gatgaaactg tccgaacagt ggctgttcac gaaggatttg gcaatcctct
    17821 tccccttcat tggcccgatc atgaagcttc cgcaccgccc gacgaagccc accgccgtga
    17881 tcgacaccgc cgccctgccc ctcttcctcg ccgccctcgc cgccgcctac ctgctgccgg
    17941 ggccggacat ggccctcatc gtgtccacca ccgcccgcca cggcccgcgc agtggcctga
    18001 tggccgccgc cggcctggcc ctgtcccgca gcctgcatgt gctcctctcc ggattcgggc
    18061 tggccgccct cttcgccgcc catcccctgc tcttccacgg cgtcaagctg ctcggcgccg
    18121 cctatctgct cggcctggcg tggacactcc tcaccgcccc cgacaccacg cccggcgcga
    18181 ccgccgcagc cgcctgcgca tcgccgcccg gcgcggcaga cgccctgcgc cgcggattcc
    18241 tcaccaatct gctcaacccc aaggccctcg gcttctgcgc catcctgctg ccccagttcg
    18301 tcgcgcccca ccagcccctg cccggccagt tcgcggtgct cgggctcatc ctcgtggggc
    18361 ttggcgcggc cttcgacgcg gcctacatct gtgccgccca gggggtggcc cgccgctttg
    18421 ccgcggcacc gcgcctgcgc gggctgtcgc gcctcacctg cgccggcgtc ttcggcctgg
    18481 ccgcgctgcg cctcgccttc agcggcaccg actgaagcct cccactgcgc cccgcgccgg
    18541 ccatcaccaa cccgcctcag cccatcaacc agcgcccgac gcccagccac agggccgtga
    18601 cgcccagcat cgccgcaaag gccgtgcgca tgcgccgctc gggcaggcga tgggcccacg
    18661 ccacgcccgc cgacaccgtc gccagcccgc ccacggccat gggcaggccc cgcgaccagt
    18721 ccacatgccc cgccgacgca tacgcggcca gcgccaccgc cgagctgggc gccaccaggg
    18781 ccagcgacaa gccctgcgcc accgcctgcc gttgccccag ccagccggcg agcaaaggcg
    18841 tcgccagcaa accgccgccg atgcccagca aacccatgct gcacccgccc gccgccccca
    18901 ccaggggcag ccagcgcaac gccaacggct tgccgacccc cgccgccgcc ggcgccgcgc
    18961 gccacaactt gatcgccaag gccagcagga acacgcagaa caccccgcgc agcaccagcg
    19021 ggtccaactg cgtcgcgaaa tgggccatgc cccaggtgac cagcgtcgcc gagccggcaa
    19081 tggccgcctg ggcccgccac ggcatgggct ggcgctgctg gtaacgccac cagcccacca
    19141 gcagattcgg caccatcagc accagggccg tcccctgggc ctgcgcctgc tccatgccaa
    19201 agcccagcac gaacaggggg atcgccagga tgcccccgcc gatgccgaag agccccccga
    19261 agaagcccag ccccgccccc accaccaggg ccagccccat gtgggcgtcc actacaaagt
    19321 ccatgcgcgt gcccccttca aaaaccgatc cgcgcacttt cattgattca ccgcatcgct
    19381 acaatcgtcc gcactgcaat tcatccctgc gtacacggca cgaatgaacg acctcccgga
    19441 cctcgccttc ttcgccctcc tcgcccgcca gcccaccctg gccgccgcgg cccaggccct
    19501 gggcgtcacg ccctccagcg tgagccgtcg cctcgccgcg ctggaacacc gcctcggcgt
    19561 gcgcctcctc caccgcacca cgcgacgcat cagcctgagc gcggaaggca gccgctacct
    19621 ggaaggcggc gaacagatcc tgcacgacct ggacaacctg gagcgctccc tgctcggcag
    19681 ccgcgaaatg ccccgcggcc tgctgcgcat caacgccacc ttcggcttcg gccgtcgcca
    19741 cctggccccg gccctttccg acttcgcgcg acgctatccc gacgtggaag tgatcctcga
    19801 tctcaccgac cgccccctcg acctgaccga acatgccgtg gacgtgggca tccgcttcgg
    19861 ccccccgccc gacgcccgca tccaggcccg ccgcatcgcc cccaaccgcc gcctcgtatg
    19921 cgccgcgccc gcctacctgg acgcccacgg ccaccccgaa tccccccgcg acctgccgcg
    19981 ccaccgctgc atcgtcatcc gcgaagccac cgccgcctac aaccactggc agctcaccga
    20041 cggacacgaa cagatcatgg tcaaggtcgg cggccccctc tccaccaact tcggcgagat
    20101 cgccgtcgaa tgggccctcg ccggccacgg catcgtgctg cgctccgaat gggacgtggc
    20161 cgccgacctc cgcgacggcc gcctgcaacg cctcctcagc ccctgggccg gcacgtccgc
    20221 cgacatccac gcggtctatc cgccgcggca tcatctgtcg gcgaaggtga aggtgtttgt
    20281 ggcgttt
//
LOCUS       DAOQMM010000068        18983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1072_length_18983_cov_47.035292, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000068 DAOQMM010000000
VERSION     DAOQMM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1072_length_18983_cov_47.035292"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="PLH85_11500"
     CDS             complement(<1..1489)
                     /locus_tag="PLH85_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65234.1"
                     /translation="MSFTRLRLDTPYPGIRPFREDEEHLFFGREAQVDALVDTLARGR
                     FLAVIGSSGCGKSSLVNCGLRPALHRGLLAQAGSAWRMATCRPGSQPLRALAESLAAP
                     GALHRDLPDSPFRLAELIESTLAMSRRGLVEAFLQAGLPAGTQLLVIVDQFEELFRYR
                     GLGPRGDAQPDADGDPATAFVNLLLEARAQDVPIHVVLTMRSDFLGDCARFAGLPEAI
                     NQGQYLVPRMSRDERRAAIAGPARVCGADIAPTLLTRLVNDVGDDPDQLSLLQHALAR
                     TWAHWRASRTPSPIEARHYDAVGTLAHALNQHAEELYAALPPGRQRLCAQAFRALTDT
                     GSDHRGVRRPCRFADLVALGGAPAEELATTLAPFRAPGAAFLMPPEGVALTADTVVDL
                     AHESLMRIWDRLRAWGEREAEAARMLRRLADAADLHARGEASLWRDPELQFALDWRQH
                     NTPNPAWAALYGRALAPALAFLDASRDARDAAAAAEIARAEAEQRT"
     gene            complement(1486..2973)
                     /locus_tag="PLH85_11505"
     CDS             complement(1486..2973)
                     /locus_tag="PLH85_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB65235.1"
                     /translation="MKYARQIFISYAHLDNQPLTADQAGWVSLFHATLQTLLSQRLGA
                     PADIWRDDKLRGNDVFSDEILGQLAGTALFVSILTPRYLKSEWCAREIRSFCERAEAE
                     HSLFVDNKSRLIKVLKLPVDGDGLLPPAAAQLLGYEFYEFDEDRTPRELDPAYGEQPR
                     QAFLRKANKLAWDLAAQINALADTPAANDGNATPPAAERPAVFLAECSRDRREARERI
                     EAELACCGYTVLPDQRLPDDEDEHVAAVDALLARCVLSIHLIGSGSGRIPDGARQQSV
                     VVLQNERAAARSRSAGLRRVIGLPPGLASPLPAQQAFLDALRQDAALQAGADLVEGDL
                     EVLKAAIHAALKPPEAPPTAPSVSVRRVHLVCDARDRKDILPFIRLLRAHGVEVTLPV
                     FSGDAGAVREANRQLALDCDVLVLWYGAGDEAWKYHQGNDLQKLCASQRERPLRGQFV
                     LLAPPLGDDKAFLRALAEADTLDALDGLDAAVLAPLLAALGTEAP"
     gene            complement(2970..3809)
                     /locus_tag="PLH85_11510"
     CDS             complement(2970..3809)
                     /locus_tag="PLH85_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNB65236.1"
                     /translation="MRLLVCTKRDLHGARLLNRLLPSLTGHELVGVWLSDKQRPAEAE
                     LPELAELACLERHLPVDLLFPLVDALPPALADTAPAASFHGLARRHGVNLSVFDELPD
                     AATRLAALAPDLVIVARFSHLFDAAALAVPRFGLLNLHPGHLPRYAGLHAPLRSVLEG
                     ETQLACSVHWITAGIDDGPLVAVHRTPFDPAGGLYTQIGELYALALPTVQAVIAACAA
                     GERPPGTPQDRRLRRYRSLPGPDDFRAFAARGLRLWQADAYRAQLAAFLPPGLALPGG
                     LPR"
     gene            complement(3812..4846)
                     /locus_tag="PLH85_11515"
     CDS             complement(3812..4846)
                     /locus_tag="PLH85_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB65237.1"
                     /translation="MKRLAYLAPALAALAVLLTAQGAAHAAPAEHPPYRIAMLLYRGC
                     EEACRGFQAYFRQHGIAAEFTLRDAAQDRRRIAGFVQELRREKPDLVVTWGTTVTEEA
                     AGRWNAVDPRRHLTDVPVVFMIVSDPVAAGIVQRLDKPRRTLTGTLYLLPVETQIKAA
                     HSYLAFRTLGYILNETESNSITTRDALRALAPSQGFTLVERALPVSAAGTPDATALPA
                     LLDALKGAGVDLLYQGPDSFLNSRRDVLTDGALARGMPVFAAAEAPVQNGRALFGVVN
                     KYEDVGRFTAAQALRILRDKVSPAAIPVELPRNFSYLINLPVALQLGRYPPLKLLDVA
                     EIVGLDGADR"
     gene            complement(4843..7155)
                     /locus_tag="PLH85_11520"
     CDS             complement(4843..7155)
                     /locus_tag="PLH85_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB65238.1"
                     /translation="MPPRAEPPSLPAAPPPDLRQWLRIGLLLLAALGLALSLQAVLLF
                     HKERDLLTGITVSRIGLLAEQIHSSFQRASANGLQLAEFRALAARLPALAASAQDVTA
                     IQVLDEDGRRLFATGPTDLPVPSPPRAARGLWHATAPLGYMWIGRTLQDSTGESVGSV
                     LIAVRSAALDARIEAARAVAGPRLALTLGLVAVALPVLLVLAMRLPGPFSLRRRVLGA
                     ALCLATAGSLHLSLAALPSFSERLAPALDDKAHALACFLSTRIDAALALGIPFERLNG
                     VEAYFTDALAQHPEVLALRLQGPTHRYAASRPGEAGHALEVPISAGVRATGATLVAVT
                     DAGVVARELRALAVDLAIVFLVALVLFNEALCAILGSARFAAGPRERLGPARLAVFLL
                     ILAEELTRAFLPVHIRALVAQTAAEDALTGPLVGLPISAYMASFALLTPFAGHWAVRF
                     GVARTFALGAGLSAAGFLGAMAGGGYGAFVAARCLCAAGYAIGTLAIQQHFLSTATAS
                     ERTRALALFVGAVQTAAICGSPVGGLLAERFGATAVFAAAAAMSGLAFLVQRLAVPVA
                     PPAAGAPAPRLLPLLTRGRVVIPLLTAALPAKVVLAGFLFYLVPLALAGADYSPASTG
                     RAMMLYFVLVAAANPLASWLSDRFGWQRSLVIAGGLVIGAGGVLGLLDRIETLFAGIV
                     ALGLGTGLSAAALQALASRDGPAAIVLLRTVERLGAVIGPLFVGSLLAVTAWGGAMAT
                     VGATMLVATLAFALWARAAASLDTPDKEPS"
     gene            complement(7311..8351)
                     /gene="ftsY"
                     /locus_tag="PLH85_11525"
     CDS             complement(7311..8351)
                     /gene="ftsY"
                     /locus_tag="PLH85_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNB65239.1"
                     /translation="MFGFLKKTPKADDAPASPDAPVATPASPAAAPEKKSWMDRLRAG
                     LSRTRQQIGGGLSSLFSRRKIDEELLEELESTLLMADCGVEATQYLIDELRRRWKRDK
                     LETADQLQGALAEGLLSILAPLEKPLEISGHSPYIIMIAGVNGSGKTTSIGKLAKHFQ
                     NQGKSVLLAAGDTFRAAAREQLMTWGERNGVAVIAQESGDPAAVVFDAINAARARGID
                     IVLADTAGRLPTQLHLMEEIAKVRRVIQKAEPSGPHEVLLVLDANIGQNALQQVKAFD
                     AAIGVTGLVVTKLDGTAKGGVVAAIGRQCPKPLRFIGVGEQIDDLQTFRAREFVDALF
                     APAGGAPADPPA"
     gene            8512..9900
                     /locus_tag="PLH85_11530"
     CDS             8512..9900
                     /locus_tag="PLH85_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB65240.1"
                     /translation="MNPRGRLASTLAALCAVAGLAVVAPARANPFEMVLPNGMKLVVK
                     EDRRAPSVVHMVWYRVGAMDEVDGTSGVAHVLEHMMFKGTKAVGPGEFNKRVAAAGGR
                     DNAFTSYDYTAYFQQVPKDALPGMMALEADRMAHLRITDAEFRKELEVVKEERRLRTD
                     DKPRALVIEQLMASAFQAHPYRRPIIGWMNDLDAMTAQDARDWYVRWYVPNNATLVVV
                     GDVDHAAVFREAAATYGRVSARPLPERKPLREPEQSGPRSTVVKAPAELPYVALAWHV
                     PTLRDVKADGDYYALQVLAAVLDGYEGARLAKNVVRGSRVAVSAGAGYDGTARGESLF
                     VLDGAPAPGKTVADVEAALRGELARIQNEGVSEDELRRVKVQAVAAQVYKRDSLMGQA
                     MEIGMDEMVGLSWKDDDYQLARLRAVTAAEVQAVARKYFSDDGVTVARLDPLPVDPEA
                     RARAAAAAPHRH"
     gene            9923..11269
                     /locus_tag="PLH85_11535"
     CDS             9923..11269
                     /locus_tag="PLH85_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB65241.1"
                     /translation="MMISVSRFGAALALTLFGLCGGAHAGPAIQTWSTPNGARVFYVE
                     SHALPLVDVQVDFRAGGAESPAGKTGVAGLTHALLDNGAGDLDEEAIANRIADLGAQM
                     GGSADMDRASITLRTLSSAAERDGAVDLLATVIQHPTFPAASFEREKARTLAGLKESD
                     TQPDAILSRRFAAAVFPGHPYGRLPTVDSVAALNRDDLAAFHARTYTAARAVVSIVGD
                     VSRADAEAMAAKLTADLPRGEAPATSVNTVLPEAQTIRVAHPSAQAHIAIGQPGIRRG
                     DPDFFPLLVGNYVLGGGGFVSRLTKEVREKRGYAYSVFSYFMPQRDVGPFEIGLQTKG
                     SQTDDALQVVNATLAEFLDKGPAGAELQAAKDNLVNGFALRLDSNRKVLEQVAVIGGF
                     GLPLDYLDRFPDKVRAVTAAQVRDAFRRHVSPAHLVTVVVGGDGDKAAAPRAEVAR"
     gene            11266..11802
                     /gene="rsmD"
                     /locus_tag="PLH85_11540"
     CDS             11266..11802
                     /gene="rsmD"
                     /locus_tag="PLH85_11540"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNB65242.1"
                     /translation="MSRIRIIGGEWRSRLIDVSTVKGLRPTPDRVRETLFNWLGQDLD
                     GRTCLDLFAGSGALGFEAASRGAAAVTMVEQDPVAFAALQKNTKTLLASRVELVRGDA
                     LKFAGSASRRFDVVFFDPPYRAGWIDRMAPFLPDLLTEHGVIYVEAEYKIENFDRLTR
                     IKHGVAGQVYYHLFAAQA"
     gene            11836..12327
                     /gene="coaD"
                     /locus_tag="PLH85_11545"
     CDS             11836..12327
                     /gene="coaD"
                     /locus_tag="PLH85_11545"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNB65243.1"
                     /translation="MRDSVAVYPGTFDPFTRGHEDLVRRAARLFDRVIVGVAASGSKG
                     PIFSMEERVEIAREVLTAYPNVEVQGFSCLLMEFLRRNEARVILRGLRAVSDFEYEFQ
                     MAGMNRKLFADVETVFLTPADEYMFISATMVREIARLGGDVSKFVQPGVNARLVQKIN
                     YSK"
     gene            12344..12595
                     /locus_tag="PLH85_11550"
     CDS             12344..12595
                     /locus_tag="PLH85_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNB65244.1"
                     /translation="MALMITDECINCDVCEPECPNGAISQGEEIYQIDPNKCTECVGH
                     FDEPQCQQVCPVDCIPFNPEFPETKDDLMEKFLKLTADK"
     gene            complement(12676..14628)
                     /locus_tag="PLH85_11555"
     CDS             complement(12676..14628)
                     /locus_tag="PLH85_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNB65245.1"
                     /translation="MLAEHFSAYTQWRGEVASGITDLRKWLQQNEVGDAQSDLRLQYL
                     LDRLREDKLIVAFVAEFSRGKSELINAVFFAGYGNRILPSSAGRTTMCPTELQWEDGD
                     TPCIRLLPIDSRRSNASISELKRFPDEWRVRPLDTSSAESLQAALAQVGETRIASREE
                     AEALGFPIDESGEKGIRPDTGGTVEIPAWRHAVIQFPHPLLEQGLVILDTPGLNAIGA
                     EPELTLNLLPNAHAVLFILAADTGVTQSDLAVWREHVNAGRRQRGRLIALNKIDGLWD
                     GLRSEADIEREIAGQVASVAATLDVAPEQIYPVSAQKGLVAKVNNDDDLLTRSRLPEL
                     EYALSTELLPTKRDIVRDNTYGEVQDIIARCRELLGARLSGLREQLTELTDLRGKNQN
                     VIEYMMRKVRSEKDEFEQGLQKYYAVRSVFTNLSNNLFGHLGLDALRDETRRTREAML
                     DAAFSRGLRGAMKGFFQHLRNNLKKSTDEIAEISKMLDAMYKRFTVEHGLKLAAPSSF
                     STLRYEKEIERLEDAFNSQFDTVLNIVTTEKHTLTQKFFETVAVQARRTFEIANRDAE
                     QWLRAVMAPLETQVREYQLQLKRRLESVKRIHQATDTLENRVNELQQAETQLVRQLAE
                     LQALSERVEHALDLPPIGEPAQPLAA"
     gene            complement(14734..15546)
                     /gene="mutM"
                     /locus_tag="PLH85_11560"
     CDS             complement(14734..15546)
                     /gene="mutM"
                     /locus_tag="PLH85_11560"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNB65246.1"
                     /translation="MPELPEVETTRRGIAPELTGRTATGALIRNGRLRLPVPEDLDAC
                     ITAQPLLAVERRAKYLLLRFTSGTVLIHLGMSGSLRVLSLEQPPGKHDHVDLCFGERI
                     LRLRDPRRFGLVLWYTGPDDAHPLLAGLGLEPLSEAFDGTWMHRASRGRATPVKLFLM
                     DGRQVVGVGNIYASESLFRAGIDPTTPVGSLGPRRCARLVNAIRETLTDALAAGGSSL
                     RDFVGAGGQPGYFQQQYFVYGRNGEPCRQCATPIRKRIMGQRATFYCPRCQR"
     gene            15727..17451
                     /locus_tag="PLH85_11565"
     CDS             15727..17451
                     /locus_tag="PLH85_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB65247.1"
                     /translation="MSPRPSAFPVRVLVAAACFATAVPAAAQLGGDDAETSSAAALPG
                     QVLTPQIFYQVMLAEVAAARGQGAAGAQAYLDLARRTRDPRLARRAAELAALSRKADL
                     AGEAAQLWLELDPASPQAQQVAAGFMASSAARIDALRSQLAKALATQGDQTGPVLLSL
                     NRGLARIPDKALVRTLVFELTEPYLLLPEAHFARSNAAYVAGDARGALTAIDSALAIR
                     PDWEQAVLLKAQLLQEVVAPAQGQTYLKDYLAAHPDGREARLTLARMLVADRQFGAAL
                     EQFRALLQRLPDDRDVVHATALLSMQQGDTASAEKLFRHLLDLGFGDADQIRAYLGQI
                     AEDGKRPDEALGWYRSVKPGTQYLPAQTHIAQILAAQGRLGEARQHLHQGARENPADR
                     VAYLLTEAQILRDAGRNAESFELLDEALRTRPDDPDLLYESALMAERLGHIDVMEGRL
                     RKLIALKPEHAHAYNALGYSLADRGLRLDEAETLIARGLALSPEDPFILDSLGWVRYR
                     KGDLAGALAALQKAYGVRADPEIAAHLGEVLWTLGRRDEAAQTWRDALKAHPDNTVLS
                     DVLKKFKP"
     gene            17448..18026
                     /locus_tag="PLH85_11570"
     CDS             17448..18026
                     /locus_tag="PLH85_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HNB65248.1"
                     /translation="MRRACHVLIATAVLALAGCAGVPSGPPVGVPGVRPAEPLQRFAL
                     EGRLQVKDGERLAAVGVSWRHERGHDEWLFTGPLGQGLARLESGPEGARLSFADGRHQ
                     DAAAATELAAAVLGVEAPFEALPAWVTARLREGAEARAVDAVARPTRVVDAGWTVEYV
                     DYVGPEPDARPRRIDIHRGDTRLRLVVDDWNP"
     gene            18028..18888
                     /gene="ispE"
                     /locus_tag="PLH85_11575"
     CDS             18028..18888
                     /gene="ispE"
                     /locus_tag="PLH85_11575"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNB65249.1"
                     /translation="MNPTDFDPSMSVCVPAPAKINLFLHIVGRRADGYHLLQTAFRML
                     DWGDEITLKRRDDGVIRRTADVPGVPAERDLVVRAAQALQAASGTRYGADIGVSKRIP
                     MGGGLGGGSSDAASVLLALNRLWGCGLSRTRLQEIGLTLGADVPFFIFGETAFAEGVG
                     EALVPLAVPPAWYVIVAPPVEVPTAKIFSAPGLTRDSELIKMRDFATDTTRNDMQATV
                     CAMFPEVAEALVWLSSRGAARMSGSGACLFAAFSSKAEAEQVAAAAPQGLKVWVAPGL
                     DRHPLWAWVG"
     gene            18907..18983
                     /locus_tag="PLH85_11580"
     tRNA            18907..18983
                     /locus_tag="PLH85_11580"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18941..18943,aa:Gln,seq:ttg)
BASE COUNT         2849 a         5997 c         7276 g         2861 t
ORIGIN      
        1 ccgtgcgctg ttcggcctcg gcgcgggcga tctcggcggc ggcggcggcg tcgcgggcat
       61 cgcggctggc gtcgaggaag gccagcgccg gggccagtgc gcgaccatag agggcggccc
      121 aggcggggtt gggggtgttg tgctggcgcc agtcgagggc gaactggagt tccgggtcgc
      181 gccacaggct ggcttcaccg cgggcgtgca ggtcggcggc gtcggcgagg cggcgcagca
      241 tgcgggcggc ctcggcctcc cgctcgcccc aggcgcgcag gcggtcccag atgcgcatga
      301 ggctctcgtg ggccaggtcc acgacggtgt cggcggtgag ggcgacgcct tcgggcggca
      361 tcaggaaggc ggcgcccggc gcgcgaaagg gggcaagggt ggtggcgagc tcctccgctg
      421 gcgcgccgcc gagggccacc agatcggcaa aacggcaggg gcgacggacg ccccggtgat
      481 cgctgccggt atcggtgagg gcgcggaagg cctgggcgca caggcgctgg cggcccggcg
      541 gcagggcggc gtagagctcc tcggcgtgct ggttgagggc gtgggcgagg gtgccgacgg
      601 cgtcgtagtg gcgcgcctcg atggggctgg gcgtgcggct ggcgcgccag tgcgcccagg
      661 tgcgggcgag ggcgtgctgg agcagggaga gctggtccgg gtcgtcgccg acgtcattca
      721 cgaggcgggt gagcagggtg ggggcgatgt cggcgccgca cacccgcgcc ggcccggcga
      781 tggcggcgcg gcgttcgtcg cggctcatgc gcggcaccag atattgcccc tggttgatgg
      841 cttccggcag gccggcgaag cgggcgcagt cgccgaggaa gtcggagcgc atggtgagca
      901 cgacgtggat cgggacgtcc tgggcgcggg cttcgaggag gagattgacg aaggcggtgg
      961 cggggtcgcc gtcggcgtcg ggctgcgcat cgccacgggg gccaaggccg cggtagcgga
     1021 acagttcttc gaactggtcg acgatgacga ggagctgggt gcccgcgggc aggccggcct
     1081 ggaggaaggc ttcgacgagg ccccggcggc tcatggcgag ggtggattcg atgagctcgg
     1141 cgaggcggaa ggggctgtcg ggcagatccc ggtgaagggc gccgggggcg gccagggact
     1201 cggcgagggc gcgcaggggc tggctgccgg ggcggcaggt ggccatccgc caggcgctgc
     1261 cggcctgggc gaggaggccc cggtgcaggg ccgggcgtag accgcaattg acgagggagg
     1321 acttgccgca gccggagctg ccgatcacgg cgaggaagcg gccgcgggcg agggtgtcca
     1381 cgagggcgtc cacctgggcc tcgcggccga agaagaggtg ttcctcgtct tcgcggaagg
     1441 ggcggatgcc cggataaggc gtatcgaggc gcaggcgggt gaagctcatg gggcctccgt
     1501 gccgagggcc gccagcagag gggcgaggac ggcggcgtcg agcccgtcga gggcatccag
     1561 cgtatcggcc tcggcgaggg cgcggaggaa cgccttgtca tcgccgagcg gcggggcgag
     1621 gagcacgaac tggccccgca gggggcgctc ccgctggctc gcgcagagct tctggaggtc
     1681 gttgccctga tggtacttcc acgcctcgtc gccggcgccg taccacagga cgaggacgtc
     1741 gcagtcgagg gcgagctggc ggttggcttc gcgcacggcg ccggcatcgc cgctgaagac
     1801 gggcagggtg acctccacgc catgggcgcg cagcaggcgg atgaagggca ggatgtcctt
     1861 gcggtcgcgg gcgtcgcata ccaggtgcac gcggcgtacg ctgacggagg gcgcggtggg
     1921 cggtgcctcg ggcggcttga gggcggcgtg gatggcggcc ttgaggactt ccaggtcgcc
     1981 ttccacgaga tcggcgccgg cctgcagggc ggcgtcctgg cgcagggcgt cgaggaaggc
     2041 ctgctgggcc ggcaggggcg aggcgagtcc cggcggcaga ccgatcaccc ggcgcaggcc
     2101 ggcgctgcgg ctgcgggccg cagcgcgttc gttctgcagg acaacgaccg actgctggcg
     2161 cgcaccgtcc gggatgcgcc cgctgccgct gccgatgagg tggatggaca gcacgcagcg
     2221 ggccagcagg gcgtctacgg cagccacgtg ctcgtcctcg tcgtcgggca ggcgctggtc
     2281 ggggaggacg gtgtagccgc agcaggcgag ctcggcctcg atgcgttcgc gggcctcgcg
     2341 gcggtcgcgg ctgcattcgg cgaggaacac ggcggggcgc tctgcggcgg ggggcgttgc
     2401 gttgccgtca ttggcggcgg gcgtatcggc cagggcgttg atctgggcgg ccaggtccca
     2461 ggcgagcttg ttggccttgc gcaggaaggc ctggcggggc tgctcgccgt aggcggggtc
     2521 gagttcccgc ggggtgcggt cctcatcgaa ttcgtagaac tcgtagccga ggagctgggc
     2581 cgccgccggg ggcaggaggc catcgccgtc cacgggcagc ttgaggacct tgatgaggcg
     2641 ggacttgtta tccacgaaga ggctgtgttc ggcttcggcc cgttcgcaga agctgcggat
     2701 ctcgcgcgcg caccactcgg acttgaggta gcgcggggtg aggatggaga cgaacagggc
     2761 ggtgccggca agctggccga ggatctcgtc ggagaagacg tcgttgccac gcagcttgtc
     2821 gtcgcgccag atgtcggcgg gggcgccgag gcgctggctg aggagggtct gcagggtggc
     2881 gtggaagagg ctgacccagc cggcctggtc cgccgtgagg ggttggttgt ccaggtgggc
     2941 atagctgatg aagatctggc gggcgtactt catcggggca gacctccggg cagggcgagg
     3001 ccggggggca ggaaggcggc gagctgcgcc cggtaggcgt cggcctgcca caggcgcagg
     3061 ccgcgggcgg cgaaggcgcg gaagtcgtcg gggccgggca gggagcggta gcggcgcagg
     3121 cggcggtcct ggggtgtgcc ggggggacgc tcgccggcgg cgcaggcggc gatcacggcc
     3181 tgtacagtgg gcagggccag ggcgtagagc tcgccgatct gggtgtagag gccgccggca
     3241 gggtcgaagg gggtccggtg gacggccacc agggggccgt cgtcgatgcc cgcggtaatc
     3301 cagtggacgc tgcaggcgag ctgcgtctcg ccttcgagga cgctgcgcag gggggcgtgc
     3361 aggccggcgt agcggggcag gtggccgggg tggaggttga gcaggccgaa gcgcggcacg
     3421 gcgagggcgg cggcgtcgaa gaggtggctg aagcgggcga caatgaccag gtccggagcg
     3481 agggcggcga gccgcgtggc ggcgtcgggg agttcgtcga acacactgag gttcaccccg
     3541 tgacggcggg ccaggccgtg aaagctggcg gccggggcgg tgtcggcgag cgccggcggc
     3601 agggcatcca cgagggggaa gagcaggtcc accggcaggt ggcgttcgag gcaggccagt
     3661 tcggcgagct cgggcaactc ggcctcggcc gggcgctgct tgtccgacag ccacacgccg
     3721 acgagttcgt ggccggtcag gctgggcagc aggcggttga ggaggcgcgc gccatggagg
     3781 tcgcgcttgg tgcagacgag caggcgcatg gtcagcggtc cgctccgtcc aggccgacga
     3841 tctcggccac gtcgaggagc ttgaggggcg ggtagcgtcc gagctggagt gccaccggca
     3901 ggttgatgag gtaggagaag ttgcgtggca gttcgaccgg gatggccgcc ggcgagactt
     3961 tgtcccgcag gatgcgcagg gcctgggcgg cggtgaagcg gcccacgtcc tcgtacttgt
     4021 tgaccacgcc gaagagggcg cggccgttct gcacgggggc ctcggcagcg gcgaaaacgg
     4081 gcatgccgcg ggccagggcg ccgtcggtga ggacgtcgcg gcgggaattg aggaagctgt
     4141 cggggccctg gtacaggaga tccacgccgg cgcccttgag ggcatccagc agggccggca
     4201 gggcggtggc gtccggcgtg ccggcggcgg agacgggcag ggcgcgctcc accagggtga
     4261 aaccctgact gggcgccagg gcgcgcaggg cgtcgcgggt ggtgatggag ttggactcgg
     4321 tctcgttgag gatgtagccg agcgtacgga acgccagata gctgtgtgcc gccttgatct
     4381 gcgtctctac gggcaacagg tagagggtgc cggtgagcgt gcggcggggt ttgtcgagac
     4441 gctgcacgat gcccgcggcg accgggtcgg agacgatcat gaagaccacc gggacgtccg
     4501 tgaggtggcg cctgggatcc acggcgttcc agcgtccggc ggcctcttcg gtgacggtgg
     4561 tgccccaggt gacgaccaga tcgggcttct cacggcgcag ttcctgcacg aagccggcga
     4621 tgcggcggcg gtcctgggcg gcgtcccgca gggtgaattc ggcggcgatg ccgtgctggc
     4681 ggaagtaggc ctggaagccg cggcaggcct cctcgcagcc gcggtagagg agcatggcga
     4741 tgcggtaggg cggatgttcg gccggggctg cgtgcgcggc gccctgggcc gtcaggagca
     4801 cggcaagggc ggcgagggcg ggggcgaggt acgcgaggcg cttcacgaag gctccttgtc
     4861 cggcgtgtcg agggacgctg cagcccgcgc ccacagggcg aaggccaggg tggcgaccag
     4921 catcgtcgcg cccacggtgg ccatcgcgcc gccccaggcg gtgacggcca gcaggctgcc
     4981 gacaaacagc gggccgatca ccgcgccgag gcgttcgacg gtgcgcagca gcacgatggc
     5041 ggccgggccg tcgcggctgg ccagggcctg caaggccgct gccgacaggc cggtgccaag
     5101 gcccagggcg acgatgccgg cgaacagggt ctcgatccgg tccaggaggc ccagtacgcc
     5161 accggcgccg atcaccagcc cccccgcgat gaccaggctg cgctgccagc cgaaacggtc
     5221 cgacagccag gatgccagcg gattggcggc cgccaccagc acaaaataca gcatcatggc
     5281 ccgtccggtc gacgcggggc tgtagtctgc accggccagg gcgaggggca cgaggtagaa
     5341 caggaagccg gcaagcacca ccttggccgg caaggcggcg gtgaggagcg ggatgacgac
     5401 gcgtccgcgg gtcagcaggg gcagcaggcg cggtgccggg gcgccggctg cgggcggcgc
     5461 gacgggtacg gcgaggcgct ggacgaggaa ggccaggccg ctcatggcgg cggcggcggc
     5521 gaacaccgcg gtggcgccga agcgttcggc gagcagcccg cccacggggc tgccgcagat
     5581 cgcggcggtc tgcacggcgc cgacgaagag ggcgagggca cgggtgcgct cggaggccgt
     5641 ggccgtgctc agaaagtgct gctggatggc cagggtgccg atggcgtagc cggcggcgca
     5701 caggcagcgg gccgccacga aggcgccgta gccgccgccg gccatcgccc cgaggaagcc
     5761 cgccgccgac aggccggcgc cgagggcgaa ggtgcgtgcc acgccgaaac gcaccgccca
     5821 gtgtccggca aagggggtga gcagggcgaa gctggccatg taggcggaga tcggcagccc
     5881 gaccaggggg ccggtgaggg cgtcttcggc ggccgtctgc gcgaccaggg cacggatgtg
     5941 cacgggcagg aaggcgcggg tgagttcctc agcgaggatc agcaggaaca ccgcgaggcg
     6001 cgccgggccg aggcgctccc gcgggccggc ggcaaagcgg gcgctgccga gaatcgcgca
     6061 gagggcctca ttgaagagca ccagcgccac caggaagacg atggcgaggt ccacggccag
     6121 ggcgcgcagt tcccgcgcga ccaccccggc gtcggtgacg gcgacgagcg tggcaccggt
     6181 cgcgcggaca cccgcagaga tcgggacctc cagcgcgtga ccggcttcgc cgggacggct
     6241 cgccgcgtag cggtgcgtag gcccttgcag acgcagggcg aggacttccg ggtgctgggc
     6301 gagggcgtcg gtgaagtagg cttcgacgcc gttcaggcgc tcgaagggaa tgcccagggc
     6361 cagcgcggcg tcgatgcgcg tggagagaaa acaggcgagg gcatgggcct tgtcgtccag
     6421 cgcgggcgcc agacgttcgc tgaagctggg cagcgccgcg aggctcaggt gcaggctgcc
     6481 ggcggtggcc aggcacagcg ccgcgccgag gacgcggcga cgcaggctga agggcccggg
     6541 caggcgcatg gcgagaacga gcagcacggg cagggcgacg gcgacgagcc cgagggtgag
     6601 ggccaaacgc gggccggcca ccgcgcgggc cgcttcgatg cgagcgtcga gcgctgcgct
     6661 gcgcaccgcg atcagcacgc tgcccacaga ttcgccggtg ctgtcctgga gtgtccggcc
     6721 gatccacatg taccccagag gggcggtggc gtgccacagg ccgcgggccg cgcgcggcgg
     6781 cgatggcacg ggcaggtcgg tcggcccggt ggcgaagaga cggcgcccgt cctcgtcgag
     6841 cacctggatg gccgtcacgt cctgggcgct ggcggcaagg gccggcaggc gggcggcaag
     6901 tgcgcggaat tcggccagct gcaggccgtt ggcggaggca cgctggaagc tgctgtggat
     6961 ctgttcggcg agcaggccga tgcgggagac ggtgatgccg gtgaggagat cccgctcctt
     7021 gtggaacagc agcaccgcct gcaggctcag ggccagaccg agggccgcca gcaggaggag
     7081 gccgatgcgc agccattgcc ggagatccgg cggcggggcg gccggcagcg acggtggttc
     7141 ggcgcggggg ggcatcctcg gctctccttc ggtgcgacgg cgggccgccg gcgcggggca
     7201 tgcggcaagg ataggcgaag acgccgggcg ccgcacgcct ttgcggcgca gggcgcgctg
     7261 gggccacgcg ccagggcacc cgcccggcgc cgggactcag cctgaggcga tcaggccggg
     7321 gggtcggccg gtgcgccgcc tgccggcgcg aacagggcgt ccacgaactc cctggcgcgg
     7381 aaggtctgca ggtcgtcgat ctgttcgccc acgccgatga agcgcagcgg cttggggcac
     7441 tggcggccga tggcggccac cacgccgccc ttggcggtgc cgtcgagctt ggtgacgacg
     7501 aggccggtga cgccgatggc ggcatcgaag gccttgacct gctgcagggc gttctggccg
     7561 atgttggcgt cgagcaccag cagtacctcg tgggggccgg acggctcggc cttctggatg
     7621 acccgacgca ccttggcgat ttcttccatc aggtgcagct gggtgggcag acggccggcc
     7681 gtgtcggcca ggacgatgtc gatgccacgg gcgcgggcgg cgttgatggc gtcgaacacc
     7741 actgcggccg ggtcgccgga ttcctgggcg atgacagcca cgccgttgcg ctcaccccag
     7801 gtcatgagct gctcccgcgc ggcggcacgg aaggtgtcgc cggcggccag caggacgctc
     7861 ttgccctggt tctggaagtg cttggcgagc ttgccgatgg aggtggtctt gccggagccg
     7921 ttcacccccg cgatcatgat gatgtagggc gaatggccgc tgatctcgag gggcttctcg
     7981 aggggcgcga ggatggacag caggccttcg gcgagggcgc cctggagctg gtcggcggtt
     8041 tccagcttgt cccgcttcca gcggcggcgc agttcgtcga tgaggtactg ggtcgcctcg
     8101 acgccgcagt cggccatcag cagcgtggat tccagttctt caaggagttc ctcgtcgatc
     8161 ttgcggcggg agaacaggct ggacaggccg ccgccgatct gctggcgggt gcgggacaga
     8221 ccggcgcgca ggcggtccat ccaggatttc ttttcgggcg cagcagccgg gctcgccggc
     8281 gtcgcgacgg gcgcgtccgg cgaggcgggc gcgtcgtccg ccttgggggt cttcttcagg
     8341 aaaccaaaca tggagagccg ggtcagagtg agggtcgatc gggtcggcca cgcgggtgaa
     8401 agcccttgcg tgtcctgttg gccgggtcgc cggatgccgc tatgatgccg cgccacacgc
     8461 cggccagcgg ccaccgtccc tcaattttac cagatgcttc aagagcctat gatgaatccc
     8521 cgcggacgcc tcgcgtccac cctggcggcc ctgtgcgccg tggccggtct ggccgttgtc
     8581 gccccggccc gggccaatcc cttcgagatg gtactcccca acggcatgaa gctggtcgtc
     8641 aaggaagacc ggcgggcgcc ctcggtggtc cacatggtgt ggtaccgggt gggggcgatg
     8701 gacgaggtgg atggcacctc gggcgtggcc cacgtgctgg agcacatgat gttcaagggc
     8761 acgaaggccg tcggccccgg agagttcaac aagcgcgtcg ccgcggccgg cggccgtgac
     8821 aacgcgttca ccagctacga ctacaccgcg tatttccagc aggtgccgaa ggacgcgctg
     8881 cccgggatga tggcgctgga ggccgatcgc atggcccatc tgcgcattac cgacgccgaa
     8941 ttccgcaaag aactggaagt cgtcaaggag gagcgccgcc tgcgcaccga cgacaagccc
     9001 cgcgcgctgg tgatcgagca gctcatggcg agcgccttcc aggcgcatcc gtatcgccgt
     9061 cccatcatcg gctggatgaa cgacctggac gccatgaccg cccaggacgc ccgcgactgg
     9121 tacgtgcgct ggtacgtccc caacaacgcg accctggtcg tggtgggcga tgtggatcac
     9181 gcggcggtgt tcagggaggc cgcagccact tacggccgcg tgtcggcccg cccgctgccc
     9241 gagcgcaagc ccctgcgcga acccgaacag agcggtccgc gcagcaccgt ggtcaaggcg
     9301 cctgccgagc tgccttacgt ggcgctggcc tggcacgtgc cgaccctgcg cgacgtgaag
     9361 gctgatggcg actactacgc cctgcaggtg ctggcggccg tgctggacgg ctacgagggc
     9421 gcccgcctgg ccaagaacgt ggtgcgtggc agccgcgtcg cggtgagcgc cggtgccggc
     9481 tacgacggga ccgcccgcgg cgagtccctg ttcgtgctcg atggcgcgcc agcgccgggc
     9541 aagacggtag ccgatgtgga ggcggccctg cgcggcgaac tcgcccgcat ccagaacgag
     9601 ggcgtgtccg aggacgagtt gcgccgggtc aaggtgcagg ccgtggcggc ccaggtgtac
     9661 aagcgcgact ccctgatggg ccaggccatg gagatcggca tggacgagat ggtcgggctg
     9721 tcgtggaagg atgacgacta ccagctggcg cggctgcgcg ccgtgacggc cgccgaggtc
     9781 caggccgtcg cccgcaagta cttctccgac gacggcgtga ccgtcgcccg tctcgacccg
     9841 ctgccggtgg atccggaagc ccgcgcccgt gcggccgctg cggcgcccca tcgccactga
     9901 tcgctttctg caaggactgc ccatgatgat ttccgtatcc cgtttcgggg ccgcgctcgc
     9961 cttgacgctc ttcggtctgt gtggcggcgc ccacgccggc ccggccatcc agacctggtc
    10021 aacacccaac ggcgcccgcg tcttctatgt ggaaagccac gccctgccgc tggtggatgt
    10081 gcaggtggat ttccgcgccg gcggcgccga atcgccggcc ggcaagaccg gcgtggcggg
    10141 cctcacccac gctctgctcg acaacggcgc cggcgatctg gacgaggagg ccatcgccaa
    10201 ccgcatcgcc gacctgggcg cccagatggg gggcagcgcc gacatggacc gggcgtccat
    10261 caccctgcgc accctgtcca gcgcagccga gcgggacggc gcggtggatc tgctcgccac
    10321 ggtgatccag catcccacct ttccggcggc cagcttcgaa cgggagaagg cgcgcaccct
    10381 cgccggcctg aaggaatccg acacccagcc ggacgcgatc ctgtcgcgcc gcttcgcggc
    10441 tgcggtgttt cccggccatc cctacggccg cctgcccacc gtggacagtg tggcggcgct
    10501 gaatcgcgac gacctggctg ccttccatgc ccgcacctac accgcggcgc gggcggtggt
    10561 gagcatcgtt ggtgatgtga gccgtgccga cgccgaagcg atggcagcga agctcacggc
    10621 cgatctgccg cggggcgagg cgccggcgac ctccgtcaac accgtgctgc ccgaggcgca
    10681 gacgatccgc gtggcgcacc cgtcggccca ggcccacatc gccatcggcc agcccggcat
    10741 ccgccgcggc gatccggact tcttcccgct gctggtgggc aactacgtcc ttggtggcgg
    10801 cggcttcgtg tcgcgcctga ccaaggaggt gcgcgagaag cgcggttacg cctacagcgt
    10861 gttcagctac ttcatgcccc agcgcgacgt gggccccttc gagatcggtc tgcagaccaa
    10921 gggcagccag accgacgacg ccctgcaggt ggtgaatgcg accctcgccg aattcctgga
    10981 caaggggcct gccggggccg aactccaggc cgccaaggac aacctggtga atggcttcgc
    11041 cctgcgcctc gattccaatc gcaaggtgct ggagcaggtc gcggtgatcg gcggcttcgg
    11101 cctgccgctg gactacctgg accgctttcc ggacaaggtg cgcgcggtga cggccgccca
    11161 ggtgcgcgac gccttccggc gccacgtctc gccggcgcac ctcgtgaccg tggtggtggg
    11221 gggcgacggc gacaaggcgg ccgcgccgcg ggccgaggtg gcccgttgag ccgcatccgc
    11281 atcatcggcg gcgaatggcg ctcgcgcctg atcgacgtga gcaccgtcaa gggcctgcgc
    11341 cccacgccgg atcgggtacg cgaaaccctg ttcaattggc tgggacagga tctggacgga
    11401 cggacctgcc tggatctgtt tgccggcagc ggggcgctgg gcttcgaggc ggcctcgcgc
    11461 ggtgctgcgg ccgtgacgat ggtcgaacag gaccccgtgg cctttgccgc gctgcagaag
    11521 aacacaaaaa ccctactggc gtcgcgtgta gagcttgtcc ggggcgatgc gctaaaattc
    11581 gccggctccg cctcccgccg gttcgacgtg gtctttttcg acccacccta ccgcgccggc
    11641 tggatcgacc ggatggcccc gttcctgccc gatctgctga ccgaacatgg cgtgatctac
    11701 gtggaagcgg aatacaaaat cgaaaatttc gaccgattga cacgcatcaa gcacggcgtg
    11761 gccgggcagg tgtattacca cctcttcgcc gcccaggcgt gaatttcacc ggtcgcgcag
    11821 acaaggggat acatcatgcg ggactcggtc gcggtctacc caggtacctt cgatccgttc
    11881 acccgtgggc acgaggatct cgtccggcgg gcggcccggc tgttcgaccg ggtcatcgtc
    11941 ggcgtggcgg cgagcggcag caagggaccg atcttttcca tggaagagcg ggtcgagatc
    12001 gcccgggaag tcctgacggc ctatcccaac gtggaagtgc agggattttc ctgcctgctg
    12061 atggaattcc tgcgccgcaa cgaggcccgc gtgatcctgc ggggactgcg cgccgtatcg
    12121 gatttcgagt acgaattcca gatggcgggc atgaaccgca agctcttcgc ggatgtggaa
    12181 accgtgtttc tgacgccggc ggatgaatac atgttcatct ccgccaccat ggtgcgggaa
    12241 atcgcccgcc tcggcggcga cgtcagcaag tttgttcagc cgggcgtgaa tgcgcggctg
    12301 gtgcagaaga tcaactacag caaatgacga ggaagttagc gatatggcct tgatgattac
    12361 cgacgaatgc atcaactgcg acgtctgtga gccggaatgc cccaatggtg ccatttccca
    12421 gggcgaggag atctaccaga tcgaccccaa caagtgcacc gagtgcgtcg gccatttcga
    12481 cgagccccag tgccagcagg tctgcccggt ggattgcatc cccttcaacc cggagtttcc
    12541 ggaaacgaaa gacgatctga tggagaagtt cctcaagctc acggccgaca agtaagacgc
    12601 cgcctgaccg ccgaaggaca gacgccggcc gtgcagtgat gcacggccgg cgtcgtttga
    12661 tggtccgggc cggactcagg ccgcgagcgg ctgcgccggt tcgccgatgg gcggcagatc
    12721 gagggcgtgc tcgacgcgct cggagagggc ctgcagttcg gctagctgcc ggacgagctg
    12781 ggtttcggcc tgctgcagtt cgttgacacg attttcaagg gtgtcggtgg cctggtgaat
    12841 gcgcttgacg ctttccaggc ggcgcttgag ctggagctgg tattcccgca cctgggtttc
    12901 gagcggcgcc atcacggccc gcagccattg ctccgcgtcc cggttggcga tctcgaaggt
    12961 gcggcgggcc tgcaccgcga cggtctcgaa gaacttctgg gtcagggtgt gcttctcggt
    13021 ggtgacgatg ttcagcaccg tgtcgaactg gctgttgaag gcgtcttcga ggcgctcgat
    13081 ctccttctca tagcgcaggg tggagaagga cgagggtgcg gccagcttga gcccgtgttc
    13141 caccgtgaaa cgcttgtaca tggcgtcgag catcttcgag atctcggcga tctcgtcggt
    13201 ggatttcttg aggttgttgc gcaggtgctg gaagaagccc ttcatggcgc cgcgcaggcc
    13261 ccgcgagaag gccgcgtcga gcatggcttc gcgggtgcgg cgggtttcgt cgcgcagggc
    13321 atccagtccc aggtggccga acaggttgtt ggacaggttg gtgaatacgc tgcgcaccgc
    13381 gtagtacttc tgcaagccct gctcgaactc gtccttttcg gagcgcacct tgcgcatcat
    13441 gtattcgatg acgttctggt tcttgccgcg caggtcggtg agttcggtga gctgctcacg
    13501 caggccggac aggcgggcgc cgagcagttc gcggcagcgc gcgatgatgt cctggacttc
    13561 accgtaggtg ttgtcgcgca cgatgtcccg cttggtgggc aggagctcgg tggacagggc
    13621 atattccagt tcgggaaggc ggctgcgggt gaggaggtcg tcgtcgttgt tgaccttggc
    13681 caccagccct ttctgggccg agaccgggta gatctgttcc ggcgccacat cgagggttgc
    13741 ggcgacgctg gctacctgtc cggcgatctc gcgctcgatg tccgcctcgc tgcgcaggcc
    13801 gtcccacagg ccgtcgatct tgttgagggc gatgagccgt ccgcgctggc ggcgtccggc
    13861 gttgacgtgt tcgcgccaga cggccagatc gctctgggtg acacccgtgt cggcggcaag
    13921 gatgaacagc accgcgtggg cattgggcag caggttgagg gtcagctcgg gctcggcgcc
    13981 gatggcattg agccccgggg tgtcgaggat caccaagccc tgctcgagca gcgggtgggg
    14041 aaactggatg acggcatggc gccaggccgg gatctccacc gtgccccccg tgtccgggcg
    14101 gatgcccttc tcgccgctct cgtcgatggg aaatccgagc gcctcggcct cctcgcggga
    14161 ggcgatgcgg gtttcaccca cctgggcaag ggccgcctgg aggctttccg cggaactggt
    14221 gtctagcgga cgtacccgcc attcgtccgg gaagcgcttg agctcggaga tgctggcatt
    14281 gctgcgccgg ctatcgatgg gcagcagacg gatgcagggc gtatcgccgt cttcccattg
    14341 cagttcggtg gggcacatgg tggtgcgccc ggcgctggaa ggcaggatcc ggttaccgta
    14401 acccgcaaag aacaccgcgt tgatgagttc ggacttgccc cgcgagaact cggccacgaa
    14461 ggccacgatc agcttgtctt cccgcagccg gtcgagcagg tactggaggc gcaggtcgga
    14521 ctgggcgtca ccgacttcgt tctgctgcag ccacttgcgc agatcggtga tcccggaagc
    14581 cacctcgccg cgccactggg tgtacgcgga aaagtgctcg gcaagcattt gggcgtcttt
    14641 cacttgcagg gtccctggct ggtggccgtg aaatacggca cattttgctc ttcattctaa
    14701 gcgcaaatcg ggaaaattcg caccggccgc cgctcatcgc tggcaacggg gacaataaaa
    14761 cgtggcgcgt tgccccatga tccgcttgcg gatcggtgtg gcgcactggc ggcagggttc
    14821 tccattccgg ccatagacga aatactgctg ctggaagtag ccgggctggc cgcctgcgcc
    14881 gacgaaatcc cgcaggctgc tgccgcccgc ggcgagggcg tccgtcaggg tctcgcggat
    14941 ggcgttgacg aggcgggcgc agcggcgtgg cccgaggctg ccgacgggcg tcgtcgggtc
    15001 gataccggcg cggaacaggc tttccgatgc atagatgttg cccacaccga cgacctgtcg
    15061 cccgtccatc aggaacagct tgacgggcgt ggcccgcccg cggctggcgc ggtgcatcca
    15121 ggtgccgtca aaggcctcgg agaggggctc cagccccagg ccggcaagca gggggtgggc
    15181 gtcgtcaggc ccggtgtacc acaggaccag cccgaagcgg cgggggtcgc gcagacgcag
    15241 gatgcgctcg ccgaagcaca gatccacgtg atcgtgtttc ccgggcggct gctcgaggga
    15301 aagtacgcgc agactgccgg acatgcccag atggatcagc acggtgccgg aggtgaagcg
    15361 caagagcagg tatttggccc ggcgctccac cgcgaggagg ggctgggcgg tgatgcaagc
    15421 gtccaggtcc tccggtaccg gcaggcgcag acggccgttg cggatgagcg cgccggtggc
    15481 ggtccggccg gtgagctcag gagcgatgcc gcggcgcgtg gtctcgactt cgggcagttc
    15541 gggcatggcg gatgcaatgg agaggcgggc aagggggccg gcttgctggg gggctggaaa
    15601 gcgcctaaca tccggcgaac ccaattgtag cggcgtgatc caacccgttg tccacccacg
    15661 ccggggcctt gcgggtcggg gccacgccca ccgcaggcgc tccgccagtc agtccgagtc
    15721 atgccgatga gcccccgtcc ttctgcgttc cctgtccgcg tcctggtggc cgcggcgtgc
    15781 tttgccacag ccgtgccggc cgccgctcaa ctggggggcg acgacgcgga gaccagttcg
    15841 gccgcggccc tgccgggtca ggtgctgacg ccccagatct tctaccaggt catgctcgca
    15901 gaggtggcgg ctgcccgcgg tcagggcgct gccggggccc aggcttacct ggacctcgcc
    15961 cggcgtaccc gcgacccacg cctggcgcgg cgggccgccg agctggctgc cctcagccgc
    16021 aaggccgacc tggcgggcga agccgcccag ctctggctgg agctcgaccc cgcgtcgccc
    16081 caggcccaac aggtggcggc aggcttcatg gcatccagtg ccgcccggat cgacgcgctg
    16141 cgcagtcaac tggccaaggc tctcgccact cagggcgatc agaccgggcc ggtgctactg
    16201 tccctcaatc gcggtctggc ccggattccc gacaaggcct tggtgcgcac gctggtcttc
    16261 gagctcaccg aaccttatct gctgctgccg gaggctcatt ttgcccgttc caatgccgcg
    16321 tacgtggccg gcgacgcgcg gggcgcgttg acggccatcg acagtgccct ggcgatccgc
    16381 ccggactggg agcaggccgt gcttctcaag gcccagctgc tccaggaggt ggtcgcgccg
    16441 gcgcaggggc agacctatct gaaggattat ctggcggccc atccggatgg gcgggaggcg
    16501 cgcctgacgc tggcccgcat gcttgttgcc gatcggcagt tcggagccgc gctggagcag
    16561 ttcagagcgt tgctgcaacg cctgccggac gaccgggacg tggttcacgc gactgcgctg
    16621 ctgtccatgc agcagggcga cacagccagc gcagaaaagc tcttccggca tctcctcgat
    16681 ctgggtttcg gcgacgcgga ccagatccgc gcctatctcg ggcagatcgc cgaggacggc
    16741 aagcggcccg acgaagcgct cggctggtac aggtccgtga agccgggaac ccagtacctg
    16801 ccggcccaga cgcacatcgc gcagatcctc gcagcccagg gcaggctcgg cgaggcgcgt
    16861 cagcatctgc accagggtgc ccgcgagaac cctgccgacc gcgttgccta cctcctcacc
    16921 gaggcccaga tcctgcgcga tgcggggcgc aacgccgaat ccttcgaatt gctcgacgag
    16981 gccctgcgga cccgtccgga cgacccggac ctgctctatg aatcggccct gatggccgaa
    17041 cgcctcggtc acatcgatgt gatggaaggc cgcctgcgca agcttattgc cctcaagccc
    17101 gagcacgccc acgcctacaa cgccctgggc tattccctgg cggaccgggg cctgcgtctg
    17161 gatgaggccg agacgctgat tgcccgtggg ctcgcattgt cgccggagga cccgttcatc
    17221 ctcgacagcc tggggtgggt gcgctaccgc aagggcgatc tggcgggggc gctggcggcg
    17281 ctgcagaagg cctacggcgt gcgtgccgat ccggagatcg cggcgcatct cggcgaagtg
    17341 ctgtggacgc tcgggcgtcg cgacgaggcg gcccagacgt ggcgtgatgc gctgaaggcc
    17401 catccggaca acaccgtgct gtcggacgtg ctgaagaagt tcaaaccttg agacgggcct
    17461 gtcatgtcct gatcgcgacc gcggtgctcg cgctggccgg ctgcgccggc gtgccgtctg
    17521 ggccgccggt gggcgttccc ggcgtgcgtc cggcagagcc cttgcagcga ttcgccctcg
    17581 aagggcgcct gcaggtgaag gatggtgagc gtctggcggc ggtgggtgtg tcgtggcgcc
    17641 atgagcgcgg gcacgacgaa tggttgttta ccggcccgct cggtcagggg cttgcccggc
    17701 tggagtccgg ccccgagggg gcacgtctga gtttcgccga cggccgtcat caggacgccg
    17761 cagcggcaac ggaactcgct gccgcggtgc tcggtgtcga ggccccgttc gaagccttgc
    17821 ccgcgtgggt cacggcccgt ttgcgcgagg gggcggaggc gagggcggtg gacgccgtcg
    17881 cgcgtccgac acgtgtggtg gatgccgggt ggaccgtcga gtatgtcgat tatgtcggcc
    17941 cggagcccga tgcacggccc cgccgcatcg acatccatcg gggcgacacg cggctgcgcc
    18001 tcgtggtgga tgactggaac ccctgagatg aatccgaccg atttcgatcc atccatgtcc
    18061 gtctgcgtac cggcgccggc caagatcaac ctgttcctgc acatcgtggg ccgtcgtgcc
    18121 gacggttatc atctcctgca gaccgccttc cgcatgctcg actggggcga cgagatcacg
    18181 ctgaagcgcc gcgacgacgg tgtcattcga cggaccgccg atgtgcccgg cgtgccggct
    18241 gagcgcgatc tggtggtgcg ggccgcccag gccctgcagg cggcaagcgg cactcgctac
    18301 ggtgcggaca tcggcgtgag caagcgcatt cccatggggg gcgggctggg cggtggcagc
    18361 tcggatgccg ccagcgtgct gctggccctc aaccggctgt ggggctgcgg cctgtcacgg
    18421 acgcgcctgc aggagatcgg cctgaccctc ggcgcggacg tgcccttctt catttttggc
    18481 gagacggcgt tcgcggaagg cgtgggcgaa gcgctggtgc cgctggcggt gccgccggcg
    18541 tggtacgtga tcgtggcgcc gccggtcgaa gtgccgaccg cgaagatttt ttcggcgccg
    18601 ggcttgacgc gcgatagcga actcatcaaa atgcgcgact tcgcaacgga caccacgcga
    18661 aacgacatgc aggcaacggt ctgcgcgatg tttccggagg tcgccgaggc gctggtctgg
    18721 ttgtcgtccc gaggggcggc gcggatgagt ggttcgggag cctgtttgtt tgccgccttc
    18781 agcagcaagg cagaagcaga acaggtggcc gccgcggcac cgcagggtct gaaggtctgg
    18841 gtcgctcccg ggctagacag gcatcccctg tgggcttggg tggggtgaag tgtgaaagcg
    18901 gtttcttggg gagtcgccaa gtcggttaag gcaccggatt ttgattccgg cattcgaggg
    18961 ttcgaatcct tcctccccag cca
//
LOCUS       DAOQMM010000069        18836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1082_length_18836_cov_49.075342, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000069 DAOQMM010000000
VERSION     DAOQMM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18836
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1082_length_18836_cov_49.075342"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..857)
                     /locus_tag="PLH85_11585"
     CDS             complement(174..857)
                     /locus_tag="PLH85_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNB65250.1"
                     /translation="MLLHIPHVLSRDAVADCRRQLEAAAWVDGRLTAGSQSEQRKHNL
                     QLAEDSDLARALRDTVLDALGRTPTFFSAALPRRIYPPLFNCYTGAHNAFGDHVDNAV
                     RTHRATQTHLRTDLSFTLFFTDPDEYDGGELVIEDTFGCQRIKLPAGDLVLYPSSSVH
                     RVEPVTAGARIACFSWLESMVRETERRRLLYDLDRQICRLRETHGDDDTAVGLTSCYH
                     NLVRMWACP"
     gene            complement(878..3118)
                     /locus_tag="PLH85_11590"
     CDS             complement(878..3118)
                     /locus_tag="PLH85_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNB65251.1"
                     /translation="MPAHCPIRTPNPPASALVPLGALLFGFSLPTAAAESAPTLAPVI
                     VTDRQDVPDASNSYQGQTTTVGKLKQLPKDIPQALTIVTEKLMEDRGQSTLKDALSNV
                     AGLTFNAGEGGRIGDNMNLRGFYSFGDLYQDGIRDTAQYNRDTFNLQQIDVLRGSGSM
                     LFGRGQAGGVINQVSKTAMLYDENEVSATVGSYNTQRLTADLNRRLGDTSAVRINLMK
                     TDAGSSRDGVTSDREGIAPTLRWGIGTDDEFALSHFYLKTHNVPDYGVPFYQNRPLDV
                     PASRFYGTSRDYEDNTTNLTTGSYQHRFSADSAVKTVLRAASYDRELWAAQPQLRGSW
                     SSPNTCTAFSGTLSDASTICRSIKARGAREKTLTSQTDFTTRFTTGALKHEALAGVEF
                     LREDLSRWSHSLTGIVVPATTVGAPDPGAPGLGSAYGNQVPTSPASYTGNSRGVYAQD
                     TVEFLPGWKLLAGLRHDSLRADYLTTAAGASSPTATALGFSEMSYRSGLMFQPDDETS
                     YYLAWNDSFNPTADLYQLDVGQGFPAERSRTTEIGAKWELFDGDLSLRAALYRAVKFW
                     ERNTDLESSGGLLSKKRHTDGVELEAAGRITPKWEVFGGVALMDAVIDEAGYTLNTAT
                     GVVTAHNPNLVGMRPRNAPPYTFNLWSTYKLAGGWRVGAGVEGKGERLAYGIGTGTAA
                     ITPNVAPAYQRWDAMVAYEQPAWSVRLNVLNLFDTRYYESIYDNGGHVVPGTERAVQV
                     TTRFKF"
     gene            3392..5890
                     /locus_tag="PLH85_11595"
     CDS             3392..5890
                     /locus_tag="PLH85_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB65252.1"
                     /translation="MQYPAARPTQPLKPLVITLAAAWGASALPALADDATRAPTVVVV
                     GQTPLPGLDQPIFQIPAPVQFATDKDVDRVRVPDLSAFMNRSLSGVHINEIQGNPFQA
                     DVNYRGYTASPLAGTPQGLSVYLDGVRINQPFGDVVSWDMIPRSAIASVTLMPGSNPL
                     FGLNTLGGALALTTKDGRTHPGASVQVTGGSYGRRSVEFEHGGFNDKGLEWFVTGNLF
                     HEDGWRAHSPSDVHQFFGKAGWQNATTDIDLSLAVADNRLNGNGLQERRFLDQDWHSV
                     YTSPDITKNNSMFLNLTGRHSLSDDAILSGNAYYRRIAGTTFNGDVNEGALDQSVYQP
                     NAAERSALAAAGYSGYPTSGANASNTPFPSWRCIANALLNDEPNEKCTGLLNRTSTTQ
                     RNFGLSGQLSVAGTLAGRPNQFTAGAAYDQSQVNFRQSAQFGYINPDHTITPVDAYAD
                     GTQTSENAFDARVDLNGRTRTWSVFATDTLALAEKWHATLSGRYNTTTVKNRDALNPG
                     GGSASLDGDHRFSRFNPAAGITFSPTPALNAYLGYTEGSRTPTAIELGCANPDNPCKL
                     PNAMAGDPPLNQVVTRTWDAGLRGRLGAETRWNAGVFRAVNSDDILFVADNQSGYGYF
                     RNFGKTRRQGVELGASTRVGALDLSANYTWLDATYQSEELLGGSGNSSSDAASAGLDG
                     NIRVRPGDRIPLIPRQILKLSADYRVTPALAVGADLVGVSGSTARGNENGQHQADGTY
                     YLDGGRSAGYSVINLNSTYQASRGVQLFGQIGNLFDRRYTTAAQLGSTAFSSSGGVLA
                     RPFASVGGEFPLVHSTFYAPGAPRTLWVGLRYSL"
     gene            5918..7243
                     /locus_tag="PLH85_11600"
     CDS             5918..7243
                     /locus_tag="PLH85_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNB65253.1"
                     /translation="MIRLLMGRVGWTTLVCLAVFLALAAWRAEVDIRREGQGAAGTVR
                     LLEALAALQASDGASRAARLDELRQLGQAGRLRHLRFQLEDADGRVLVAAEGAPVAGG
                     RVVLKTADGQAYRVALSPDARSEQEEAAANIGGMAALFMAYGLVLLAGLYLAARHALK
                     PLRRLLEAIASLRQRDFSVRVPPLPVQELDSIGAALNHLGEGLAAAETRQRALHARLL
                     TVQDDEQARLALRVQESFGQELTALRSRVACLRRQSGNDPVLAGQLAALETSCRTLDA
                     SVRGMLRDLHPDVCAGGAGDGASLAPLLHELVKSWQDAAGPGTEIHEAVMPPDRTLPG
                     AVSRTLYRMTQEALANVVRHASARCVSVSVRDVPDDAAAIEWEVRDDGCGIADLDAAM
                     GRGSGLAALRERVWALGGWLQIGPANAAEGASAVSPGLCLRARLPVLAR"
     gene            7240..7884
                     /locus_tag="PLH85_11605"
     CDS             7240..7884
                     /locus_tag="PLH85_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB65254.1"
                     /translation="MIAGSPLRVVLADDHAIVRTGYRQLLEMEEDIVVVADFAHSDAV
                     CHWFAEHEADVLVLDLSMPGRGGLETLARVKAHRPALRVLIFSMHDSAAMVRQALRAG
                     ADGYLSKSSEPDALIQAVRTVARGGRPLSPDVAAAVSAGEGQPASLALSPREFEIFRL
                     LAAGRSVDEVAERLFLSAKTVANYQTTIRHKTGLAGPVDMYRHARAHGLLDGSE"
     gene            complement(7916..9178)
                     /gene="lplT"
                     /locus_tag="PLH85_11610"
     CDS             complement(7916..9178)
                     /gene="lplT"
                     /locus_tag="PLH85_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNB65255.1"
                     /translation="MPFGFYMIMAAQFFSALADNALLIASIFMLRQMQAPQEYEPLLK
                     LFFTVSYVALAAFVGAFADSMPKWRVMLISNTIKIAGCSLMFLDVHPLIAYAVVGLGA
                     AAYSPAKYGILTEYLPHRKLVVANGWIEGLTVGAIILGTLVGGMLIKPGVSQALLGID
                     VPYIDTGIDTPGEVALVVVAVLYLIAALFNLKVPDTGVDHKPLKSSPTYLVHDFIHCL
                     KLLWRDKLGQISLATTTLFWGAGATLQFIVIKWAEQALHLDLSKASMLQGVVAIGVAL
                     GAVLAAKFVSMRASVRVIPLGIAMGIIVNVMIFVTNMHVAMVLMVLIGALSGFFVVPM
                     NALLQHRGHILMGAGHSIAVQNFNENLSILIMTGLYALLIRADVSINTVVVLFGLFVA
                     GTMFLVKRRHEANQREFDSVAQLDDVKH"
     gene            9357..10268
                     /locus_tag="PLH85_11615"
     CDS             9357..10268
                     /locus_tag="PLH85_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB65256.1"
                     /translation="MADRRLQVFHAVARQGSFTRAAEALFMTQPAVTFQIKQLEEQFN
                     TRLFDRGHGRVTLTPAGELVMAYAEKILGLGEEMEARVAEMTDDLSGVLHLGTSTTIA
                     GYWLPALVAGFKQRYPRVVPRVAVGNSQLIESRVADRTLDVGLIEIVSENPTIDRRAA
                     GQDELQVILAPNHPLARATALTATDLASHPFIHREPGNAIRDLSDQFFASAGIAPEEL
                     PIAAELGSLAAVKHLTALGFGYAIASHAAIRQEIAEGSLVSVPLAPRLYTPLEVIVLK
                     DKFRSRLVNTFADFVTAEIARLSFSPA"
     gene            10273..11352
                     /gene="alr"
                     /locus_tag="PLH85_11620"
     CDS             10273..11352
                     /gene="alr"
                     /locus_tag="PLH85_11620"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNB65257.1"
                     /translation="MPRPIRASIDLDALRHNYRLARRHAGAAAAYAVIKANAYGHGLL
                     PVARALAPEADGFALLDLADAAQLRAAGIDAPIVLLEGFFEPADLPQLTALGVSPAIH
                     SKAQLAMLERAALDRPLDVLLKINTGMNRLGFTPERVPAALQRLQALACVRRITLMTH
                     FARADDEVGVAAQLASFHRVAEGLGLPASLANSAALLRYPDARGQIVRPGIMLYGSSP
                     MPALHSAEALGLRPVMTLASEIIAVQELRPGEQVGYGGTFEARLPTRVGVVACGYADG
                     YPRHAPTGTPILVGGRRTRTLGRVSMDMLACDLTHLPEAGIGAPVTLWGAGMPADEVA
                     EAAGTISYELFCALAPRVPVGYLGA"
     gene            11354..12730
                     /gene="radA"
                     /locus_tag="PLH85_11625"
     CDS             11354..12730
                     /gene="radA"
                     /locus_tag="PLH85_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNB65258.1"
                     /translation="MAKPRSIYVCRECGGRAPRWQGQCPHCQAWNTLDESVVEPEAPA
                     NRRFAALAADTGRLQSLAEVQPRESPRVPTGIEEFDRVLGGGLVPGGVVLIGGDPGIG
                     KSTLLLQALSLLAGRLKALYVSGEESAEQVALRAVRLHVEAHGLQLLPEINLERILAT
                     LRDVKPDVAVVDSIQTVYSEALTSAPGSVAQVRECAAQLTRFAKQGGTSLVMVGHVTK
                     DGALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLKGVS
                     NPSAIFLSQHGQQVSGSCVLVTQEGTRPLLVEIQALVDSAHSPSPRRLSVGLEQNRLA
                     MLLAVLHRHAGVVCFDQDVFVNAVGGVKIAEPAADLAVLLAIVSSLTSRPLPRELVVF
                     GEVGLAGEIRPAPRGQERLRESAKLGFSVALVPRANAPKQAIPGLRVIAVERIEEALE
                     QARGLSTG"
     gene            12926..13201
                     /locus_tag="PLH85_11630"
     CDS             12926..13201
                     /locus_tag="PLH85_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB65259.1"
                     /translation="MNKTELIDALAGKTGMTKVEAGRAVDGVVEIVTEALAKGESVTL
                     VGFGTFAVGERAARVGRNPQTGAELNIPASKQPKFSAGSKLKDAVNK"
     gene            13534..14541
                     /gene="hemB"
                     /locus_tag="PLH85_11635"
     CDS             13534..14541
                     /gene="hemB"
                     /locus_tag="PLH85_11635"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNB65260.1"
                     /translation="MSPVSAVFPGTRMRRMRRDDFSRRLMREHRLSADDLIYPVFVLE
                     GQDVVQPVASMPGVSRVSLDRLLMIAEEAVALGVPALALFPVVDADKKSLGAEEAFNP
                     DGLVPRVVQALKQRFPELGVITDVALDPYTSHGQDGLIDETGYVLNDETLEVLAKQAL
                     CHARAGADVVAPSDMMDGRVARIRAELDGDRQIYTRILAYSAKYASSFYGPFRDAVGS
                     AANLGKGNKYTYQMDPANSDEAIREVALDISEGADMFMVKPGMPYLDIVRRVKTELQV
                     PTYVYQVSGEYAMLKAASANGWLNDRACALEALLAFKRAGADGILTYYALEAARWLKE
                     G"
     gene            complement(14693..15247)
                     /locus_tag="PLH85_11640"
     CDS             complement(14693..15247)
                     /locus_tag="PLH85_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB65261.1"
                     /translation="MAPSLFDSRPELRTALAAACVAAVLAGCGSAPPRTTSQPPPSVI
                     APPERAWFSLPHPDHAQEVVLYALGLLDTGYRFGGRNPEAGLDCSGMVSFIVEQVSGQ
                     RLPHNAAQIAERTRPVASGALQPGDLVFFNTLGRAYSHMGIYLGDGRFIHAPNSRGKV
                     RVDRLDNRYFAERFEGGRTLLPRG"
     gene            complement(15216..15653)
                     /gene="dtd"
                     /locus_tag="PLH85_11645"
     CDS             complement(15216..15653)
                     /gene="dtd"
                     /locus_tag="PLH85_11645"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNB65262.1"
                     /translation="MIAVIQRVLEARVTVDGRIVGAIGPGLLALVAVEKGDDSPRTAR
                     MAERLMSYRVFADEAGRMNRSLAETAGGLLLVSQFTLAADTARGTRPSFTPAAAPEDA
                     RRLFDELVRHARVRHPDVATGEFAADMKVALINDGPVTFRLTT"
     gene            complement(15704..16411)
                     /locus_tag="PLH85_11650"
     CDS             complement(15704..16411)
                     /locus_tag="PLH85_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990055.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNB65263.1"
                     /translation="MGLKHLTACLAILTMNLTAARAADAPPTVTAPLADLSAEASRSA
                     PNDQLRALVYAEASDANAADVARRVNGLIAQALATARGYPAVKVKSAGNSTTPVYGKS
                     GRAIDAWRMRSSLSLESGDTAALSELLGKLQQSLAVASLNAAPSPETWRKVEDEVIGD
                     ALSAFQNRARLVAGGLGRGWRIRQISVNTSGGRPQPVMPYARAAMAIAEAAPAPVEAG
                     DTPITVTVTGQIELLDR"
     gene            complement(16506..17309)
                     /locus_tag="PLH85_11655"
     CDS             complement(16506..17309)
                     /locus_tag="PLH85_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNB65264.1"
                     /translation="MAHSNLPTDDEARAAELPFPTLREVGFAAPFAWVGRGLADLKAN
                     PTASLFYGFCFAGMGLLVTFIFEHAYQYTSAATTGFLLMAPFFAMGLYELSRRRELGQ
                     PVQLGPTLTVWRRNAGNIAVFAAVLTVIFLIWARASLVVFAIFYTRELPNLSGFLTQV
                     LSFENIEFLAVYFAVGMIFALLVFAVSVVSIPLMLDRNQDAVTSMIASIRALALNPGP
                     LLVWAVLIVALTILGFLSFHLGLVVTMPIVGHATWHAYRDLVAPLPRHG"
     gene            complement(17366..18199)
                     /locus_tag="PLH85_11660"
     CDS             complement(17366..18199)
                     /locus_tag="PLH85_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB65265.1"
                     /translation="MDLRVTLSPNAPSECPVFAYTGGRSPAPDRPWIVFLHGAGNDHS
                     VWMLQTRAFAARGWNVLAPDLPGHGRSGGQALDTIEALADWTLRLLDAAAIERCALVG
                     HSMGSLIALHLASIAPERVFRLALVGSVFPMPVAPALLDKARIDPPAAHALINQWSFA
                     PATLLGRSAVPGIHPAAVNERLLQRQPVGTLPVALQACNAYAGGFEAAARIHCPTLLL
                     SAAQDRMTPPKALAPLADAFTSSAMPQRVVIPGAGHAMMNEAPTAVLEALERFMTPPA
                     G"
     gene            complement(18349..>18836)
                     /locus_tag="PLH85_11665"
     CDS             complement(18349..>18836)
                     /locus_tag="PLH85_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB65266.1"
                     /translation="FGACLSPMNAFQILQGIETLGLRMERHVANAREIVQRLLGHPQV
                     ENIAWPELDGHPDQALARRLLPKGCGGVVSFRLKGGRPAGRAFIGALRLFSHLANVGD
                     AKSLALHPASTTHFRMTPEALAAAGIGEGTIRLSVGLESVSDLLDDVDRGLHAAQKAT
                     G"
BASE COUNT         2972 a         5903 c         6864 g         3097 t
ORIGIN      
        1 gacgctgctc tggggcggga cctgcggcgc tgcggtgtcg agggcgaggt gcacgatcag
       61 tgcgccggcg agggcgtgga gcccgcaggc cgctgcgatg ccggcactct gccgcaggcg
      121 cgcgggatgg ccggggcgcg gtgggtgcgg caggggcagg gcgagggcgc tgctcatggg
      181 caggcccaca tgcggacgag gttgtgatag cagctggtca gaccgacggc agtgtcgtcg
      241 tcgccgtggg tctcgcgcag acggcagatc tgccggtcca ggtcgtagag caggcggcgc
      301 cgctcggtct cgcggaccat gctttccagc cagctgaagc aggcgatgcg cgcgccggcc
      361 gtgacgggct cgacgcgatg cacgctggac gaggggtaga gcaccaggtc gccggcgggc
      421 agcttgatgc gctggcagcc gaaggtgtcc tcgatgacca gctcgccgcc gtcgtattcg
      481 tccgggtcgg tgaagaacag ggtgaaggac aggtcggtgc gcaggtgggt ctgggtggcg
      541 cgatgggtgc gtacggcgtt gtccacgtgg tcgccgaagg cgttgtgggc gcccgtgtag
      601 cagttgaaga ggggcgggta gatgcgtcgc ggcagggccg cggagaagaa ggtgggggta
      661 cggccgaggg cgtcgagcac ggtgtcgcgc agggcacggg cgaggtccga gtcctcggcc
      721 agttgcaggt tgtgcttgcg ctgttcggac tggctgcccg cggtgagcct gccatcgacc
      781 caggcggcgg cttcgagctg gcgccggcag tcggcgacgg cgtcgcggga caggacgtgg
      841 gggatgtgca ggagcatggg cagacctcgt cggatgttca gaacttgaag cgggtggtga
      901 cctggacggc gcgttcggtg cccggcacga cgtggccgcc gttgtcgtag atggactcgt
      961 agtagcgcgt gtcgaacagg ttgagcacgt tcaggcgcac gctccaggcg ggctgttcgt
     1021 aggccaccat ggcgtcccag cgctggtagg cgggggcgac gttgggggtg atggcggcgg
     1081 tgccggtgcc gatgccatag gcgaggcgct cgcccttgcc ctccaccccc gccccgacgc
     1141 gccagccacc ggcgagcttg taggtgctcc acaggttgaa ggtatagggc ggggcgttgc
     1201 gcgggcgcat gccgaccagg ttggggttgt gggccgtgac gacgccggtc gcggtgttga
     1261 gggtgtatcc ggcctcgtcg atgactgcgt ccatcagggc gacgccgccg aagacttccc
     1321 atttcggggt gatgcggccg gcggcctcca gttcgacgcc gtcggtgtga cgcttcttgc
     1381 tgagcaggcc gccgctggat tccaggtcgg tgttgcgctc ccagaacttg acggcccggt
     1441 acagggcagc gcgcagggac aggtcgccgt cgaagagttc ccacttggcg ccgatctcgg
     1501 tggtgcggct gcgctcggcc gggaagccct gacccacgtc gagctggtag aggtcggcgg
     1561 tggggttgaa ggagtcgttc caggccaggt agtagctggt ctcgtcgtcg ggctggaaca
     1621 tcaggcccga gcggtagctc atctcggaga agccgagggc ggtggccgtg gggctgctgg
     1681 cgccggccgc ggtggtcagg tagtcggcgc gcaggctgtc gtggcgcagg ccggcgagga
     1741 gcttccagcc gggcaggaat tcgacggtgt cctgggcgta cacgccgcgg gagttgccgg
     1801 tgtagctggc cgggctggtc ggcacctggt tgccgtaggc gctacccaga cccggtgcgc
     1861 cggggtcggg cgcgccgacg gtggtggcgg gaacgacgat gccggtgagg ctgtggctcc
     1921 agcggctcag gtcttcccgc aggaattcga cgccggccag ggcttcgtgc ttgagggcgc
     1981 cggtggtgaa gcgggtggtg aagtcggtct ggctggtgag ggtcttctcg cgggcgccgc
     2041 gggccttgat ggagcggcag atcgtgctgg cgtcgctgag ggtgccggaa aaggcggtgc
     2101 acgtattggg cgacgaccag ctgccccgta actgcggttg ggcggcccac agctcgcggt
     2161 cgtagctggc ggcccgcagc acggtcttga cggcgctgtc ggcggagaag cgatgctggt
     2221 agctgccggt ggtcaggttg gtggtgttgt cttcgtagtc ccggctggtg ccgtagaagc
     2281 ggctggcggg aacgtccagg gggcgattct ggtagaaggg cacgccgtag tccgggacgt
     2341 tgtgggtctt caggtagaag tggctgaggg cgaactcgtc gtccgtgccg atgccccagc
     2401 gcagggtggg ggcgatgcct tcccggtcgg aggtgacgcc gtcgcgggaa ctgcccgcgt
     2461 cggtcttcat gaggttgatg cgcacggcgc tggtgtcgcc caggcggcgg ttcaggtcgg
     2521 cggtgaggcg ctgggtgttg tagctgccga cggtggcgct gacctcgttc tcgtcgtaga
     2581 gcatggccgt cttgctcacc tggttgatca cgccgccggc ctggccgcgg ccgaagagca
     2641 tggagccgga gccgcgcagg acgtcgatct gctgcaggtt gaaggtgtcc cggttgtact
     2701 gggcggtgtc gcggatgccg tcctggtaga ggtcgccgaa ggaatagaag ccgcgcaggt
     2761 tcatgttgtc gccgatgcgg ccgccttcgc cggcattgaa ggtgaggccg gctacgttgg
     2821 agagggcgtc cttgagggtg gactggccgc ggtcctccat cagcttctcg gtgacgatgg
     2881 tgagggcctg gggaatgtcc ttgggcagct gcttgagctt gccgacggtg gtggtctggc
     2941 cctggtagct gttggaggcg tcgggaacgt cctggcggtc cgtgacgatc accggcgcga
     3001 gggtcggggc gctttcggct gcggcggtgg ggaggctgaa cccgaagagc agggcgccca
     3061 ggggaacgag ggcggacgca ggcggattcg gggtacggat ggggcagtga gcgggcatgt
     3121 cggtctccag tcggggctgg ctggcttgcg acaggccgcg ggggctgtgc tggctggctg
     3181 ggctgcaggg aagggttgaa aggcgtggcc gtcggtgttg aaggcttctt aaatgcgaat
     3241 gattatcaat atatagagtg tgccggtggg ctgtcaacgg cgtcagcttc ggagggcgct
     3301 gaatccgtgt gtcgcgggag tttctcccgt agcgcaaagg acggcgtctg agtagtctct
     3361 gcccgcacgc aaaaaaacac ggagacttcc tatgcagtac cctgccgcgc gcccgacgca
     3421 gcccctcaaa cccctcgtga tcaccctggc cgctgcctgg ggcgcgtccg cgctcccggc
     3481 gctggccgac gacgccacgc gcgcccctac ggtggtcgtg gtgggccaga cgcccctgcc
     3541 gggcctggac cagccgatct tccagattcc cgcgccggtg cagttcgcca ccgacaagga
     3601 cgtggaccgg gtccgtgtgc cggatctgtc ggccttcatg aaccgcagcc tgagcggcgt
     3661 gcacatcaac gagattcagg gcaatccctt ccaggcggac gtgaactacc gcggctacac
     3721 cgcatctccc ctggccggca caccccaggg gctgtcggtg tacctggacg gggtgcgcat
     3781 caaccagccc ttcggcgacg tggtgagctg ggacatgatc ccccgctcgg cgattgccag
     3841 cgtaacgctg atgcccggct cgaatcccct gttcggcctc aataccctgg ggggggcgct
     3901 ggccctcacc accaaggacg ggcgcaccca tccgggcgcc tcggtgcagg tcacgggggg
     3961 ctcctacggg cgccgctcgg tggaattcga gcacgggggc ttcaacgaca aggggctgga
     4021 atggttcgtc accggcaacc tgttccacga ggacggctgg cgggcccatt cgccctcgga
     4081 cgtgcaccag ttcttcggta aggccggctg gcagaacgcc accaccgaca tcgacctgag
     4141 cctcgccgtg gcggacaacc ggctcaatgg caacggcctg caggagcggc gtttcctcga
     4201 tcaggactgg cacagcgtct acaccagccc ggacatcacc aagaacaact cgatgttcct
     4261 caacctgact ggccgccaca gcctgtcgga cgacgcgatc ctgtcgggca acgcctacta
     4321 ccggcggatt gccggcacca ccttcaacgg tgacgtgaac gagggcgccc tcgaccagtc
     4381 ggtctatcag cccaatgcgg ccgaacgttc ggcgctggcg gcagcgggtt actccggtta
     4441 ccccaccagc ggcgccaacg cctccaacac gccgtttccg tcctggcgct gcatcgccaa
     4501 cgccctgctc aacgacgagc ccaacgagaa gtgcaccggc ctgctcaacc gcaccagcac
     4561 cacccagcgc aatttcggcc tgagcgggca gctgtcggtg gccggcaccc tggccggacg
     4621 cccgaaccag ttcaccgccg gggcggccta cgaccagagc caggtgaact tccgccagtc
     4681 ggcccagttc ggctacatca acccggatca caccatcacg ccggtcgatg cctacgccga
     4741 cgggacgcag acgtccgaga acgccttcga tgcgcgcgta gacctgaatg gccgtacccg
     4801 cacctggagc gtttttgcca ccgacacgct ggccctggcc gagaaatggc acgccaccct
     4861 atccggccgc tacaacacga ccaccgtgaa gaaccgggac gctctcaatc cgggcggtgg
     4921 cagcgcgtcg ctggacggcg atcatcgctt cagccgcttc aatccggcgg ccggcatcac
     4981 cttcagcccg acgccggccc tcaacgccta cctgggctac accgaaggca gccgcacacc
     5041 taccgccatc gaactggggt gtgccaaccc ggacaatccg tgcaagctgc ccaacgccat
     5101 ggccggcgat ccgcccctca accaggtggt gacgcgcacc tgggatgcgg gcctgcgcgg
     5161 ccgcctgggg gccgagacgc gctggaatgc cggcgtcttc cgggcggtga actcggacga
     5221 catccttttt gtggccgaca accagtccgg ctacggttac ttcaggaatt tcggcaagac
     5281 ccgccgccag ggcgtggagc tgggggccag cacccgcgtc ggcgccctcg atctgtccgc
     5341 caactacacc tggctggacg ccacctacca gagcgaggag ctcctcggag gcagcggcaa
     5401 cagttccagc gatgccgcca gcgccgggct ggacggcaac atccgggtcc gcccggggga
     5461 tcgcattccg ctgattccgc gccagatcct caaactgtcg gcggactacc gggtgacccc
     5521 ggcgttggcg gtgggcgccg acctggtcgg cgtgtccgga tcgacggccc gcggcaacga
     5581 gaacggccag catcaggcgg atggcacgta ttacctggac ggggggcgct cggccggcta
     5641 ctcggtgatc aacctcaact ccacctatca ggccagccgc ggcgtgcagc tcttcggtca
     5701 gatcggcaac ctcttcgacc gccgctacac cacggccgcc cagcttggat ccacggcctt
     5761 ctccagcagc ggtggcgttc tcgcccggcc ctttgcgtcg gtgggcgggg aattcccgct
     5821 ggtgcattcc accttttacg cgccgggcgc gccgcgcacc ctgtgggtgg gcctgcgtta
     5881 cagcctctga ccgggtgagg ggaggcggcc cgtggacatg atccgcctcc tcatgggccg
     5941 ggtgggatgg acgaccctgg tgtgcctggc cgtattcctg gcgctcgccg cctggcgggc
     6001 cgaggtggac atccgccgcg aagggcaggg cgccgccggc acggtacgcc tgctcgaagc
     6061 cctcgccgcc ctgcaggcca gcgacggggc gagccgcgcg gcccgtctcg acgagttgcg
     6121 gcaactgggg caggcgggcc gcctgcgcca cctgcgcttc cagctggagg acgcggacgg
     6181 tcgcgtcctg gtggcggctg aaggtgcgcc cgtggcaggc ggtcgggtcg tcctcaagac
     6241 ggccgacggg caggcctatc gggtggccct gagccccgac gcccgcagcg aacaggaaga
     6301 ggccgccgcc aatatcggcg gcatggcggc gctcttcatg gcctatgggc tggtactgct
     6361 ggccgggctg tatctggccg cgcgccacgc cttgaagcca ctgcgtcggc tgctggaggc
     6421 cattgccagc ctgcgccagc gggatttcag cgtgcgggtg ccgccgctgc cggtgcagga
     6481 actggacagc atcggtgcgg ccctcaatca tctcggcgag ggattggccg cggcggagac
     6541 gcgccagcgt gcgctgcacg cgcggctgct taccgtgcag gacgacgagc aggcgcgcct
     6601 ggccctgcgt gtgcaggaga gcttcggaca ggaactcacg gccctgcgca gccgggtggc
     6661 ctgcctgcgg cgccagagcg ggaacgatcc ggtcctcgcc ggccagctgg cggcgctgga
     6721 gacgtcctgt cggacgctgg atgcgtcggt gcggggcatg ctgcgggatc tgcaccccga
     6781 cgtctgcgcc gggggggcgg gcgacggggc gtccctggcg ccccttctcc acgaactcgt
     6841 caaaagctgg caggatgcgg ccggtccggg aaccgagatc catgaggcgg tgatgccgcc
     6901 ggatcggacc cttcccggcg ccgtgtccag aaccctctac cgcatgaccc aggaagccct
     6961 cgccaacgtc gtccgccacg cctccgcacg ctgcgtgagc gtgtccgtgc gcgacgttcc
     7021 ggacgacgct gcggccatcg agtgggaagt tcgcgatgac ggctgcggca ttgccgatct
     7081 ggacgcggcg atgggacgtg gcagcggtct cgcggcgctg cgtgagcggg tctgggccct
     7141 gggcgggtgg ctgcagatcg gacccgcgaa tgcagcagag ggtgcctcgg cggtgtcgcc
     7201 cggcctgtgc ctgcgggccc gcctgccggt gcttgcgcga tgatcgcggg cagtccgctg
     7261 cgtgtcgtgc tggcggacga tcacgccatc gtccgcacgg gctaccgcca gttgctggaa
     7321 atggaagaag atatcgtggt ggtggccgac tttgcccaca gcgacgcggt atgccactgg
     7381 ttcgccgagc atgaggcgga tgtgctggtg ctggacctgt cgatgccggg gcggggtggg
     7441 ctggagaccc tggcgcgggt caaggcccat cgcccggctc tgcgtgtgct gatcttttcg
     7501 atgcatgaca gcgccgccat ggtccgtcag gccctgcgtg cgggcgctga cggctatctg
     7561 agcaagagca gcgagcctga cgccctgatc caagcggtgc ggacggtggc ccgcggcggt
     7621 cggcccctgt cgccagacgt ggcggccgcg gtgtcggcgg gcgaggggca gccggcctct
     7681 ctggcgctgt caccgcggga gttcgagatc tttcgcctgc tggcggccgg gcggagtgtg
     7741 gacgaggtgg cggagcggct cttcctcagc gccaagacgg tggccaatta ccagacgacg
     7801 atccgccaca agaccgggct ggccgggccg gtggatatgt accgtcacgc ccgcgcccac
     7861 ggtctcctgg acggcagcga gtaaggcgca cgggccgcca gtggcggcgc acggttcagt
     7921 gcttgacgtc gtcgagctgg gcgaccgagt cgaactcgcg ctgattggct tcgtgccggc
     7981 gcttgaccag gaacatggtg cccgccacga agaggccgaa gaggaccacc acggtgttga
     8041 tggacacatc ggcgcggatc agcagggcat agagccccgt catgatcagg atcgacaggt
     8101 tctcgttgaa gttctgcacc gcgatggagt ggcctgcccc catcaggatg tggccgcgat
     8161 gctggagcag ggcattcatg ggcaccacga agaagccgga cagggcgccg atcagcacca
     8221 tcagcaccat ggccacatgc atgttggtga cgaagatcat cacattgacg atgatgccca
     8281 tggcgatacc cagggggatc acccggaccg aggcgcgcat gctcacgaat ttggccgcca
     8341 gcaccgcacc cagcgccacc ccgatggcca ccacgccctg gagcatggag gccttggaga
     8401 gatccaggtg cagggcctgc tcggcccact tgatcacgat gaactgcagg gtggcgccgg
     8461 caccccagaa cagggtggtg gtggccaggg agatctggcc gagtttgtcg cgccagagca
     8521 gcttgagaca gtggatgaag tcgtgcacca gatatgtggg gctggatttc agcggcttgt
     8581 ggtccacgcc cgtatccggc accttcaggt tgaacagggc agcgatcagg tagagcacgg
     8641 cgaccaccac gagcgccact tcgcccggcg tgtcgatgcc cgtgtcgatg tacggcacgt
     8701 cgatacccag cagcgcctgg gagacaccag gcttgatgag catccccccg accagggtgc
     8761 caaggatgat ggcgcccacc gtgaggccct cgatccagcc gttggccacc accagctttc
     8821 gatgggggag gtattcggtc aggatgccgt acttggccgg tgaataggcg gcggcgccca
     8881 ggcccaccac cgcgtaggcg atgaggggat ggacgtcgag gaacatgagg gagcagccgg
     8941 cgatcttgat ggtgttgctg atgagcatca cccgccactt gggcatggag tcggcaaagg
     9001 cgccgacgaa ggcggcgagc gcaacgtagg agacggtgaa gaagagcttg aggagcggct
     9061 cgtattcctg gggtgcctgc atctgccgga gcatgaagat ggatgcgatc agcagtgcgt
     9121 tgtcggccag cgcggaaaag aactgcgcgg ccatgatcat gtagaaaccg aagggcatcg
     9181 tcaggcggtc gaagctcggt gaacgcagga aagggcgctt tataccacga aggacgtatt
     9241 gcccgttcgt gccagcgcgg ggcgcggtgg gggattcgtg aatttcgact gcatccctgt
     9301 ggcgcggcgg gtgtcgatgt gctgaaataa gcgcatataa accggattta tgagccatgg
     9361 ccgatcgacg actccaggta ttccacgcgg tggcccgaca gggctccttc acccgcgccg
     9421 ccgaagccct gttcatgacc caaccggccg tgaccttcca gatcaagcag ctggaagagc
     9481 agttcaacac gcgcctcttc gatcgcggcc acgggcgggt caccctcacg cccgccggcg
     9541 aactggtgat ggcctacgcc gagaagatcc tcggtctggg tgaagagatg gaagcacggg
     9601 tcgccgagat gaccgatgac ctgtccggcg tgctgcacct gggcaccagc accaccatcg
     9661 caggctactg gctgccggcg ctggtggccg gcttcaagca gcgctatccg cgcgtggtgc
     9721 cgcgggtggc ggtgggcaat tcgcagctca tcgagagccg cgtggcggac cgcaccctgg
     9781 atgtgggcct catcgagatc gtctcggaga atcccaccat cgatcgccgt gcggcggggc
     9841 aggacgagct gcaggtgatc ctggcgccga atcatcccct cgctcgcgcc actgcgctga
     9901 cggccactga cctggcgtcc caccccttca tccaccggga acccggcaac gccatccgcg
     9961 atctgtcgga ccagttcttc gcgtccgcgg gtatcgcgcc ggaagagctc cccatcgccg
    10021 cggaactggg cagtctcgcg gcggtcaagc acctcaccgc cctcggtttc ggttatgcaa
    10081 ttgcgtccca tgcggcgatc cgccaggaga tcgccgaggg ctccctggtc agcgtacccc
    10141 tggcgccccg gctgtacacc cccctcgaag tgatcgtcct gaaggacaag ttccgctcgc
    10201 gtctcgttaa cacctttgcc gatttcgtca ccgccgagat cgcccgcctc agcttttcgc
    10261 ccgcctgaca tcatgccccg tcccatccgc gcgtccatcg acctggacgc cctgcgtcac
    10321 aactatcgtc tcgcccgtcg ccatgcgggc gcggcggccg cctatgcggt gatcaaggcc
    10381 aacgcctatg gtcacggcct gctgcccgtg gcccgggccc ttgcgccgga agccgacggc
    10441 tttgccctgc tcgacctggc cgatgccgcg caactgcgcg ctgcgggcat cgacgcgccc
    10501 atcgttctcc tggaaggctt cttcgaaccg gccgatctgc cgcagctgac cgccctgggc
    10561 gtctcgccgg cgatccattc caaggcccag ctcgccatgc tggagcgggc tgcgctggac
    10621 cggcccctgg acgtgctgct caagatcaac acgggcatga atcgcctcgg cttcacgccc
    10681 gagcgggtgc cggcggccct gcaacgcctg caggccctgg cgtgcgtgag gcggatcacc
    10741 ctgatgacgc acttcgcccg ggccgacgac gaggtggggg tcgccgccca gctggcaagc
    10801 ttccatcggg tggccgaagg gctcggtctg ccggccagcc tggccaattc ggctgcgctg
    10861 ctgcgctatc cggacgcccg cggacagatc gtgcgtccag gcatcatgct ctacggcagc
    10921 tcgccgatgc cggcgctgca cagcgcggag gccctcggcc tgcgtcccgt gatgacgctt
    10981 gccagcgaga tcatcgccgt gcaggaactg cgtcccggcg agcaggtggg ctatggcggc
    11041 accttcgagg cacggttgcc gacgcgcgtg ggcgtggtgg cctgcggcta cgccgacggc
    11101 tatccccgtc acgcgcccac cggcacgccc atcctggtgg gtgggcggcg cacgcgcacg
    11161 ctggggcggg tgtccatgga catgctcgcc tgcgatctca cccatctgcc ggaggccggc
    11221 atcggcgctc ccgtgaccct gtggggggcc ggcatgccgg cggacgaggt ggcggaggcg
    11281 gcgggcacca tcagctacga gctcttctgc gccctggcgc cgcgggtgcc ggtcggctac
    11341 ctcggggcct gaggtggcga agccccgttc gatctacgtc tgccgcgaat gcggtggcag
    11401 ggccccgcgc tggcaggggc agtgtcccca ttgtcaggcc tggaacaccc tggacgaatc
    11461 cgtcgtcgag cccgaggcac cggccaaccg gcgttttgcc gcgctggccg ccgacaccgg
    11521 gcgtctgcag agtctggccg aggtgcagcc gcgggaatcc ccgcgtgtgc cgacgggcat
    11581 cgaggagttc gaccgggtgc tgggcggcgg actggtgccg ggcggcgtcg tgctgatcgg
    11641 cggcgatccg ggcatcggga aatccaccct gctgctgcag gccctgtccc tgctggcggg
    11701 gcggctcaag gccttgtatg tgagcggcga ggagtccgcc gagcaggtgg ccctgcgggc
    11761 ggtgcgcctg cacgtggagg ctcacggcct gcaactcctg cccgagatca acctggagcg
    11821 gattcttgcc accctgcggg acgtgaagcc cgacgtggcg gtggtggact cgatccagac
    11881 cgtgtattcc gaggcgctga cctcggcgcc gggctcggtg gcccaggtac gcgagtgtgc
    11941 cgcccagctc acccgtttcg ccaagcaggg cgggacgagc ctggtgatgg tcgggcacgt
    12001 caccaaggat ggggccctgg cgggcccgcg ggtgctggag cacatcgtcg acacggtgct
    12061 gtatttcgag ggcgataccc attccagttt ccgtctggtg cgggccttca agaaccgctt
    12121 cggggcggtg aacgagctgg gcgtgttcgc catgaccgag cggggcctga agggcgtcag
    12181 caatccgtcg gcgatcttct tgtcccagca cgggcagcag gtgtccggct cctgcgtgct
    12241 ggtgacccag gaaggtacgc gcccgcttct ggtggagatc caggccctgg tggacagtgc
    12301 ccacagcccg agtccgcggc ggctctcggt cgggttagag cagaaccggc tggccatgtt
    12361 gctggcggtg ctgcatcgtc acgctggcgt ggtgtgcttc gatcaggatg tgttcgtgaa
    12421 tgcggtgggc ggcgtgaaga tcgccgaacc ggcggccgac ctggcggtgc tgctggccat
    12481 cgtgtcctcc ctcaccagcc ggccgctgcc gcgggagctg gtggtcttcg gcgaggtggg
    12541 gctggcgggc gagatccgtc cggcgccgcg gggccaggaa cggctcaggg aatccgccaa
    12601 gctgggcttc agcgtggcgc tggtaccccg cgccaatgcc ccgaagcagg ccattcccgg
    12661 actgcgggtc atcgccgtgg aacggatcga ggaagccctg gagcaggcgc gcggcctgtc
    12721 gaccggttga tgtatgcgtt ttgcgcttga attgggtcag aacccgcgga aaatcgtgag
    12781 attcgcacca caggctcgtg ggagaagggt aagcttcgct ccgcgggttt atgggcacgc
    12841 aaggcaggac gcccacagat cctgcttgca aggctgcatc cacccaaatt tctacatcca
    12901 cttcttcatc taaaagttac gcgctatgaa caagactgaa ctgattgatg ctttggcggg
    12961 caaaaccggg atgaccaagg tcgaagccgg tcgtgccgtc gacggcgttg tggaaatcgt
    13021 cactgaggcg ctggccaagg gcgagagcgt gaccctggtg ggttttggta cctttgccgt
    13081 cggcgagcgc gccgcccgcg tgggccgcaa tccccagacc ggtgccgagc tgaacatccc
    13141 ggcgtccaag cagcccaagt tctcggccgg ttccaagctg aaggatgccg tgaacaagta
    13201 atgcgacgac tcggtgcgcg catggcgtac cggactcagt catgtcaggt ggtgcctttc
    13261 gcgggtccca cctgtttttt tatgggcggc gggggcgtat cctcggcgcc tggcgggttt
    13321 ccggccgagc cggattgggc gccccgaaaa agaaaacccc cggccagggg ggtgaccggg
    13381 ggctaaaacg ccttgcatgc aatcaaggcc ccgctcaggg aggtatagcg ggagacggtt
    13441 cagcaccatc tgtaccttgg tcagggtcag cggggtaaaa gttcccgggg cccttcgttt
    13501 cgagatgtaa gtctgtacgc cagcgagaat tccatgagcc cagtttccgc cgtctttccc
    13561 ggcacccgca tgcgtcgcat gcgccgggac gatttttccc gtcgcctgat gcgcgagcat
    13621 cgcctgtctg cagatgatct gatctatccg gtcttcgtgc tggaggggca ggatgtagtg
    13681 cagccggttg cctccatgcc cggtgtttcg cgcgtatccc tggaccgtct gttgatgatt
    13741 gccgaagagg cggtggcgct gggcgtaccg gccctggccc tgtttcccgt ggtggacgcc
    13801 gacaagaaat ccctcggcgc cgaagaggcc ttcaacccgg atggcctcgt gccccgcgtg
    13861 gtgcaggccc tcaagcagcg cttcccggaa ctgggcgtga tcaccgacgt ggccctcgac
    13921 ccctatacca gccacggcca ggacggcctc atcgacgaga cgggctatgt gctcaatgac
    13981 gagacgctgg aagtgctggc aaagcaggcc ctctgccacg cccgggccgg cgccgatgtg
    14041 gtggcaccat cggacatgat ggatggccgc gtggcgcgca tccgtgccga gctggacggc
    14101 gaccgccaga tctacacccg catcctggcc tattccgcca agtacgcgtc gagcttttac
    14161 ggccccttcc gcgacgcggt gggttcggcc gccaatctgg gcaaaggcaa caaatacacc
    14221 taccagatgg acccggccaa ttcggatgag gcgatccgcg aggtggccct ggacatttcg
    14281 gaaggcgccg acatgttcat ggtgaagccg ggcatgccct atctggacat cgtccgtcgg
    14341 gtgaagaccg aactgcaggt gccgacctac gtgtaccagg tgagcggtga atacgcgatg
    14401 ctgaaggcag cctcggccaa cggctggctg aatgaccggg cctgcgcact ggaggcgctg
    14461 ctggctttca agcgggcggg ggcggatggc atcctgacct attacgcgct ggaagcggcg
    14521 cgctggctga aggaagggtg aacggtctcc gtcacggtgg tcgggctctg gtgtgccggg
    14581 gccttgcggg actgccggac atccgcgctt cggatgtccg gcagtcccgc gcatgcatgg
    14641 gatcggttca ggacgctccg gagcccatgc ccatccggca tccgtgtgcg cctcagcccc
    14701 gtggcagcag ggtccgcccc ccttcgaagc gttcggcgaa gtaacggttg tcgaggcggt
    14761 cgacccgcac cttgccgcga ctgttggggg cgtggatgaa gcgcccgtca cccagataga
    14821 tgcccatgtg ggaataggcg cgtccgaggg tgttgaagaa caccagatca ccgggctgca
    14881 gggcgccgga agcgaccggg cgggtccgct cggcgatctg cgcggcgttg tggggcaggc
    14941 gctgtccgct cacctgttcc acaatgaagc tgaccatccc gctgcaatcg agcccggcct
    15001 ccggattgcg accgccgaaa cggtagcctg tgtcgagcag cccgagggcg tacagcacga
    15061 cctcctgggc atggtcggga tggggcagtg aaaaccacgc ccgttccggc ggtgcgatga
    15121 ccgatggggg cggctgggag gtcgtgcggg gcggcgcact gccgcagcct gccagcacgg
    15181 ccgccacaca ggcggcagca agcgccgtgc gcagttcagg tcgtgagtcg aaaagtgacg
    15241 gggccatcgt tgatcagggc gactttcatg tcggcggcga attcgccggt ggcaacatcg
    15301 ggatgacgga cgcgggcatg acggaccagt tcatcgaaca ggcgccgcgc gtcttccggt
    15361 gcagcggccg gcgtgaagct ggggcgcgta ccgcgggcgg tgtcggcggc cagcgtgaat
    15421 tgcgagacca gcagcagacc gccggcggtt tcggcgaggg accggttcat gcgacctgct
    15481 tcatcggcaa atacccggta ggacatgagc cgctccgcca tgcgcgccgt acgtgggctg
    15541 tcgtcaccct tctcgacggc caccagggcg aggaggcccg gaccgattgc gccgacgatg
    15601 cgtccgtcca ccgtcacgcg cgcctccagc acccgttgaa tcactgcaat catgacgcgc
    15661 agtttagcct gcgccgcgca gcattcaagc cctgttgcgg ggttcagcgg tcgagcagct
    15721 cgatctggcc ggtgaccgtg acggtgatcg gcgtgtcgcc tgcctccacc ggcgccggag
    15781 cggcctcagc aatcgccatc gccgcgcggg cgtagggcat gaccggctgg gggcgtccgc
    15841 cgctggtatt gaccgagatc tgccggatgc gccagccgcg tccaagtcca ccggccacca
    15901 gccgggcgcg gttctggaag gcggagagcg cgtcgccgat gacctcgtct tccaccttgc
    15961 gccaggtttc cggagagggc gcagcgttga gactcgcaac cgcaagactt tgctggagtt
    16021 tgccgaggag ttcggacaag gcggcagtgt ccccgctttc cagactcagg ctggagcgca
    16081 tgcgccaggc gtcgatggcc cgcccgctct tgccgtaaac cggcgtggtg ctgttgccgg
    16141 cgcttttgac cttgactgcg gggtatccgc gggcggtggc cagtgcctgg gcgatcaggc
    16201 cgttcacccg ccgcgcgacg tcggcggcgt tggcatcgct ggcttcggca tagacaaggg
    16261 cgcggagctg gtcattgggg gcgctgcggc tggcttcggc gctcaggtcg gccagcgggg
    16321 ccgtgacggt cgggggcgcg tcggcggcgc gcgcggccgt gagattcatc gtcaggatgg
    16381 caagacaggc ggtcaggtgc ttcaggccca tggggcacac tccggaaagg tgggcggaaa
    16441 cgcttttgtg ccacaggccg cgtgacggtg cagtaagcgg atgttccgcc ggtgccggcc
    16501 ggtactcatc cgtgacgcgg aaggggggcg acgagatccc gataggcatg ccaggtggcg
    16561 tgaccaacga tgggcatggt gacgacgagc ccgagatgga agctcaggaa gccgaggatc
    16621 gtgagggcca cgatcagtac ggcccacacc agcagtgggc cgggattgag ggcgagggcg
    16681 cggatgctgg caatcatcga ggtgaccgca tcctggttgc ggtcgagcat gaggggaatg
    16741 gacacgacgc tgacggcgaa caccagcagg gcgaagatca tgccgacggc aaaatacacg
    16801 gccaggaact cgatgttctc gaaggacagc acctgggtga ggaagccgct cagattgggc
    16861 agttcgcggg tgtagaagat ggcgaagacg accagcgagg cccgtgccca gatcaggaag
    16921 atgacggtca ggacggcggc aaagactgcg atattgcctg cgttacgccg ccagacggtg
    16981 agggtcgggc cgagttgcac gggctggccg agttcgcgcc gccgggacag ttcatacagg
    17041 cccatcgcaa aaaatggagc catcagcaga aagcccgtgg tggcggccga ggtgtattgg
    17101 taggcgtgct cgaagatgaa ggtgaccagc aggcccatgc cggcaaagca gaagccatag
    17161 aacaggctgg ctgtgggatt ggccttgaga tccgcgaggc cccgaccgac ccaggcgaac
    17221 ggggccgcga agccgacctc gcgcagggtg ggaaagggca gctccgctgc gcgggcttcg
    17281 tcgtccgtgg ggaggtttga gtgcgccaag ggttgtctcc gttatggttt tgtggaactg
    17341 gctgctgcag tgtggaacgt gagattcagc ccgcaggggg cgtcatgaag cgctccaggg
    17401 cttcgagcac cgcagtgggc gcttcgttca tcatggcatg accggcacct ggaatcacga
    17461 cgcgctgcgg catggcgctg gatgtgaagg cgtcggcgag gggggcgagg gccttgggcg
    17521 gcgtcatgcg atcctgggcc gcgctgagga gcagggtcgg gcagtggatc cgtgcggcgg
    17581 cttcgaatcc gccggcgtag gcattgcagg cctgcagcgc gacaggcagg gtgccgaccg
    17641 gctggcgttg gagcaggcgt tcattcacgg cggcagggtg gatgcccggt acggcgctgc
    17701 gccccagcag ggttgcgggc gcaaaggacc actgattgat cagggcgtgg gcggcgggcg
    17761 ggtcgattcg agccttgtcg aggagggccg gtgccacggg catcgggaag acgctgccca
    17821 ccagggcgag gcggaagacg cgttcgggcg cgatggaggc gaggtgcagg gcgattaatg
    17881 agcccatgct gtgtccgacc agggcgcaac gctcgatggc ggcggcgtcg aggaggcgca
    17941 gcgtccagtc cgccagggcc tcgatggtgt cgagggcttg cccaccgctg cggccatggc
    18001 cgggcaggtc gggcgcgagc acgttccagc cgcgtgcggc gaaggcgcgg gtctggagca
    18061 tccagacgct gtggtcgttg ccggcaccgt gaaggaagac gatccacggc cggtccggtg
    18121 ccgggctgcg cccacccgtg taggcgaaga ccgggcactc actgggggcg tttggcgaca
    18181 gggtgacgcg gagatccatg gatcaggccg cccgtcgggg agtttcaggg gcggcggatg
    18241 ccccctggcg atgagcgcgt gggttttcgg cgacagtgac agggcccgcc gttcggcgcg
    18301 gctggacagt ggccactgtc cgtgattgac aaaatttcaa ggtggacatc atccggtcgc
    18361 tttctgggcc gcgtgcaggc cccgatccac gtcgtcgaga aggtcggaaa cggattccag
    18421 tccgaccgag aggcggatcg tcccttcgcc gatgcccgca gcggcaaggg cctccggtgt
    18481 catgcggaag tgggtggtgc tggcggggtg gagggcgagg gatttggcgt cacccacgtt
    18541 ggccaggtga gagaacaggc gcagggcgcc gatgaaggcg cggccggccg ggcgcccgcc
    18601 cttgagacgg aagctcacga cgccgccaca gcctttcggc agcagccggc gggccagagc
    18661 ctggtcgggg tggccgtcca gttccggcca ggcgatgttt tcgacctggg gatggcccag
    18721 caggcgctgg acgatctccc gggcgttggc gacgtgacgc tccatgcgca gaccgagggt
    18781 ctcgatgccc tgcaggatct ggaaggcatt cattggcgac aggcaggcgc cgaagt
//
LOCUS       DAOQMM010000070        18601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1101_length_18601_cov_50.247223, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000070 DAOQMM010000000
VERSION     DAOQMM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18601
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_18601_cov_50.247223"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLH85_11670"
     CDS             complement(<1..200)
                     /locus_tag="PLH85_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNB65267.1"
                     /translation="MKSAEIRQKFLEFFASKGHQIVASSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATTSQKCV"
     gene            364..915
                     /locus_tag="PLH85_11675"
     CDS             364..915
                     /locus_tag="PLH85_11675"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNB65268.1"
                     /translation="MGHRLSKIYTRTGDAGTTGLGNGNRVSKNSLRIHTLGEVDEVNA
                     AVGVLLCEDLPEDVRVLLTNVQHDLFDLGGEVCIPGMEMITAKQVQYLEDELDRHNDD
                     LEPLKDFILPGGTRAAALCHQARTVCRRAERMIVALAQEEAVNDAPRQYLNRLSDLLF
                     VLGRVLNKAGGRGDVLWQKRKNA"
     gene            complement(1008..1817)
                     /gene="murI"
                     /locus_tag="PLH85_11680"
     CDS             complement(1008..1817)
                     /gene="murI"
                     /locus_tag="PLH85_11680"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNB65269.1"
                     /translation="MSETGTLPIGVFDSGVGGLTVVRALMERLPLESIVYFGDTARVP
                     YGVKSVATIEHFTGQITDHLLHQGVKMLIIACNTMAAVASHVVKDKAGDMPVLDVIDA
                     GARAAVAESRSRAIGVIGTPTTINSNAYARRMHALDPNVRVYSQACPLFVPLVEEGWL
                     EHEVTRLTAQEYLRPVLAENVDTLVLGCTHYPLIKPLLQDVVGPQVRLVDSAITVAEQ
                     AARALADLGIANPGDRLPTYRYTVTDIPLRFQTIGERFLGRSLGVVDKVDW"
     gene            complement(1904..2677)
                     /locus_tag="PLH85_11685"
     CDS             complement(1904..2677)
                     /locus_tag="PLH85_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65270.1"
                     /translation="MMSRKPAPEEERPPQTLAEVTAMESWRFDPLCIAEDDPKVSAPS
                     DSSNKFRIKMLMRDGDRRRETDLLIEKRYAWRGYGKLGSLEAPNQLTMNAELFGRVYA
                     TLTVNIDSPAGLALDKTYGAEAARLRSQGRKLCEFGRFAVDPSARSKRLIASLFNLLY
                     IYSYRVKGCTDILIEINPRHRDFYEKLLEFHQLGEVKHCARVNAPAMLMHLSCATIEA
                     RLKEIGGRWRELPDEKSIYKFAFTPEEESELIGRLDDDH"
     gene            complement(2674..3777)
                     /gene="queG"
                     /locus_tag="PLH85_11690"
     CDS             complement(2674..3777)
                     /gene="queG"
                     /locus_tag="PLH85_11690"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNB65271.1"
                     /translation="MDTGHDVLSRKAEDLAALATRIRAWGEELGFGGIGISDVALEGV
                     EEGLAAWLAAGFHGSMDYMEKHGTKRTRPAELVAGTRRVISVRMDYLSAAAAHACEVL
                     ADRSLAYVSRYALGRDYHKVLRQRLQKLADRISAAAPHGYRVFTDSAPVMEVALAEKA
                     GLGWRGKHSLLLSREAGSFFFLGEIYTDLELPVDAPRSGHCGSCRACIDACPTGAIVA
                     PYRVDARRCISYLTIELHGAIPEVYRPMIGNRVYGCDDCQWICPWNRFAGQASVSDFT
                     PRNGLDRIKLLELFNWTPQIFNERMAGSPIFRIGYERWMRNLAVGLGNAPSSAEVVRA
                     LQSRRDDQSWLVREHVAWALERHGALRCGMSWQ"
     gene            3865..4269
                     /gene="tsaE"
                     /locus_tag="PLH85_11695"
     CDS             3865..4269
                     /gene="tsaE"
                     /locus_tag="PLH85_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNB65272.1"
                     /translation="MPERLIILLHGDLGAGKTTLTRGLLRGLDYTGRVKSPTYTLVES
                     YKDSRIVVYHFDFYRFTHAEEFLEAGLDEYFQGPGLRLVEWPERAGPYLPPADLAIRL
                     RIADDGGRTLDLQALTPEGRTCVDRLSPSPRA"
     gene            4233..5657
                     /locus_tag="PLH85_11700"
     CDS             4233..5657
                     /locus_tag="PLH85_11700"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB65273.1"
                     /translation="MRRSPLTFPARLTRPSIGGNAAGFTRRRFIGAAGASLALLVSRP
                     GTAAQTGILAVRVWPAQDYTRITLESREPLTFTHQLVHGPERLVVDLEGIELNGVLQS
                     LPTKISGQDPYIQLIRAGRNRPGVVRLVIDLKAEIVPQVFTLKPVGEYGHRLVLDLYP
                     VEPPDPLLALLEKLSPEEAAAGVPMPARPEPSSVRPTRPAEPGIARRQEAEPPPVRPQ
                     PAPEPPAARRKPAEDEGVSRLVTIALDPGHGGEDPGAVGAAGSFEKHVTLSIGRRLKA
                     RIDAEPNMRAMLTRDADYFVPLQQRVSKARRVQADLFVSIHADAFISPTARGSSVFVL
                     SERGATSTAARWLAQRENAADLIGGVNLGVKDEHLARTLLDLSQTASINDSLKVGKAV
                     LGELGTINRLHKGEVEQASFAVLKAPDIPSILVETAFISNPEEEARLNDEAYQDRMAD
                     AILRGLKRYFAKNPPTAKSKLASL"
     gene            complement(5688..8036)
                     /locus_tag="PLH85_11705"
     CDS             complement(5688..8036)
                     /locus_tag="PLH85_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNB65274.1"
                     /translation="MTSGMVRGSDVASRLAFFKGLQAVTNRIHATENIDEIIFELSSE
                     ICTLFAADRLTIYLVDDNRTTISSRVKTGLQAVRTIRLPIAENSVAGYVALTGRMLNI
                     HDAYDDRELKAVSERMEFRREVDDRSGYRCHQMLVAPIMRQEGADTLGVIQLINTRSG
                     EPFSSICEEGVQGLGQTLAIAFAQRRHTLAQPKSKFESLVSGGRLSEQELREALRKAR
                     EVGKSMERVLLDDLGIPLADVGAALERFFGVPYEPYRQDRIKPLDLLKNLKRDYVQQA
                     QWLPLEDTPEGMVILTVDPEQAVASRVVQNVFPKANPVYRVTTDHEFVQTLDQFFGAA
                     EDPSVTELLSDMADDDGEGTSTAEDVSAAADNELVKLVNRIIIDAHRQGASDIHIEPR
                     PGKEKTQIRFRKDGSLVPYIEVPSSYRSPIVTRIKIMCDLDISERRKPQDGKIKFRRY
                     GPLDIELRVATVPTAGGMEDVVMRLLANSEPLPLDQLGLLEDNYRRLKDTVSKPYGIF
                     FVCGPTGSGKTTTLHSILGSINTPDVKIWTAEDPVEITQKGLRQVQVNRKAGLDFPTV
                     MRSFLRADPDVIMVGEMRDKETVSIGLEASLTGHLVFSTLHTNSAPESIVRLLDMGMD
                     PFNFADALLGVLAQRLAKRLCGKCKRPYTPDEDEIRHLLDEYCEDLHQTPAFIEDAHG
                     AKEQVLATWRQRYAKDRGPFTLYAPVGCEHCADGYKGRLGLHELMVGTDRIKALIQER
                     ARVANLLSVALEEGMRTLRQDGIEKVLGGLTDLKQVRKVCIR"
     gene            complement(8094..8864)
                     /locus_tag="PLH85_11710"
     CDS             complement(8094..8864)
                     /locus_tag="PLH85_11710"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNB65275.1"
                     /translation="MKVQVLGCSGGIGGRSQRTTSLLVDQDVLIDAGTGVGDLEFDEL
                     LKIDHVFVTHAHLDHIAMIPLLVDTVGEARSTPIVVHGSPETLRILRSHIFNWLIWPD
                     FSSIPDRGNPFLRFHEIHVGETVALDGGREITALPALHTVPAVAYRLACAHGSLVFSG
                     DTTYSEPLIAAINEIPDLRYLIVETAFPDALQGLALASRHLCPSLLALFLGRLTVNPE
                     VWITHLKPSRAQTTLDEIVSYCGRFEPRALYNGLEFAL"
     gene            complement(9019..9717)
                     /locus_tag="PLH85_11715"
     CDS             complement(9019..9717)
                     /locus_tag="PLH85_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB65276.1"
                     /translation="MPRLILSMDGLVLKEMVLEKERTTIGRKPHNDIQIDNLAISGEH
                     AAIVTILNDAFLEDLNSTNGTYVNGQPVKKHVLQNNDVVELGKYRLKFFTDSTVAAPM
                     DMTDSVAFATDLTRTDASRGAAPAPATEPPPSAAPQPAPAAMGMIQILSGSHAGRTLE
                     LSKSLTTLGKPGLQVAVITRRPHGYFITHVEGASFPVVNGRALDPQAQRLNDHDIIEI
                     AGVKMEFFSQPTER"
     gene            complement(9742..10512)
                     /locus_tag="PLH85_11720"
     CDS             complement(9742..10512)
                     /locus_tag="PLH85_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNB65277.1"
                     /translation="MADAYLPLRMAMLSDTGRVRVRNEDQVYCDASRGIALLADGMGG
                     HAGGALASRLAVAAWLAEVEDRPPGTIDETCMRAAVAAANQVVFDTACQDVLLRSMGT
                     TLVAVCFRTDGQMIACHVGDSRLYRLRGESLECLTRDHSVLREQCDAGMIEAESDGAP
                     ALRGLLTRAVGVDVQVQPDLIVTQVCAEDVFLLCSDGLTDMLPDREIFHVLDTLGANP
                     RLAAEHLVDLANDQGGTDNVSVVVVSLRSGGLPVSVIA"
     gene            complement(10537..11241)
                     /locus_tag="PLH85_11725"
     CDS             complement(10537..11241)
                     /locus_tag="PLH85_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNB65278.1"
                     /translation="MLSTPEFLTAAFWAGFVQIVAIDLVLSGDNAVVIALACRRLSPA
                     QRRIGVLWGVVGAVALRVVLTAFAASLLGYPWLKLLGGLLLLWIGVKLLLPDDDGGHE
                     IDAATSVSGAVRTIIVADFVMSVDNVIGVAGAAGDSLVLLGLGLAFSIPVIVWSSQLI
                     MKSMERFPVIVSLGAALLGWVAGSMMLSDPGLRPWVDGLPGWARMLAGALGALGVIGV
                     ARVLAMRAGRSSEQAL"
     gene            complement(11370..12263)
                     /gene="sucD"
                     /locus_tag="PLH85_11730"
     CDS             complement(11370..12263)
                     /gene="sucD"
                     /locus_tag="PLH85_11730"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNB65279.1"
                     /translation="MSILINKDTKVITQGITGKTGQFHTEKCIEYANGKNCFVAGVNP
                     KKAGEKILDVPIFGTVKEAAAETGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEG
                     IPVRDMLEVRNKMKAKEAAGGKKTLLLGPNCPGLITPEEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGLKHIDVMRMFNDDPDTDAVIMIGE
                     IGGPDEAEAAQWCKANMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTADAKLAIME
                     ECGFTVTRNPSEMGKLLKALL"
     gene            complement(12275..13435)
                     /gene="sucC"
                     /locus_tag="PLH85_11735"
     CDS             complement(12275..13435)
                     /gene="sucC"
                     /locus_tag="PLH85_11735"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNB65280.1"
                     /translation="MKIHEYQGKQILKKFGVTVPRGIHCTTVDDAVKAAETLGGKIWV
                     VKAQIHAGGRGKGGGVKVAKSLDEVRQYAGQILGMQLITHQTGPEGQKVRNLLIEEGA
                     DIKHEYYVAALTDRATQKVAMMASSEGGMDIEEVAHKTPEKIIKVFVDPAVGLTDEQA
                     LTLAKGIGIPEGSIAQAVSTLKNLYTCYMDTDASLAEINPLIHEGDGTVKALDAKFNF
                     DSNALYRHPEIVEMRDLDEEDADEIEASKFDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATTEKVTEAFKIMLKNPKVKGILVNIFGGIMRCDTIATGVVT
                     AAKEVHLSVPLVVRMKGTNEEIGKQILRDSGLPIISADTMAEAATKIVAAVQ"
     gene            complement(13716..14261)
                     /locus_tag="PLH85_11740"
     CDS             complement(13716..14261)
                     /locus_tag="PLH85_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HNB65281.1"
                     /translation="MSLPPSSAARRRVHARRIEIEGFLREDGLLDLDARLTDVKDQDY
                     VIASGLRKAGEPIHDLFLRVTIDTQFNIVGACAVSARVPYPGGCETITPAYRQLVGLN
                     LVKGFRKTVGEMFADVRGCSHLSELLLSLPTAAIQTLATFRRDTEDTDGKPFQLDRCH
                     ALETTTQTVLRYYPRWYRSGD"
     gene            14477..16486
                     /locus_tag="PLH85_11745"
     CDS             14477..16486
                     /locus_tag="PLH85_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65282.1"
                     /translation="MPRTSLLVIAVLAALPVAPVRGAALGELSARSALGAPLNAEIRI
                     TPAPGEQIDGQCIRIVRGQTDDLPWLAGARVRLEGERLIITTRDPVNHPAAMLGVHIG
                     CGIELRRDYALLLQPPLESPAVTAAVVTPDAPAPARAPSTPPAAARPRPVGQVIVLSE
                     DTPLARLADERAGPSPARRQRFIERLVMANPDVFPSGRADEGLVIAAGTRLKTTPPAL
                     PAHHADTPPRRTDTVARAQAAPAAPTSPAQATRPSTRREPAPRQDRLVVAGTAGADDM
                     PLRLSLQLANPPRDDAPGDAVRGPIRQEQRTLTEIDDRITAQLELNEKIKRLEEYQEL
                     LKRKIADLDAGPPGQAAPTAAPPPAPAPAAAPRVSTPAAVAPPESAAEGLPAWALPAG
                     AGLVTLLLGGGALVWWRRRNLVVPEDSLEPISVGAESTTGLHAETPPVFVTTMGPPSI
                     LIPSPAAAPAPQSAMPVREMPALDIDLAPKGDWSEPTFAPTHSVPFDEAVDEQDSALE
                     LAEIMMSFGRTQGAAETLADYIRNNPRQAVKPWIKLLEVYHLAGMRAEFEALTRQLNK
                     TFNVKMVSWHDYRAPETAPDSVEQLPHIVRRLEETWNTADAQAYIHQILRDNRDGTRQ
                     GFPLGVVDDLLMLLAVLDDHLGPYRQAAETPDNAPAPADTHKAAA"
     gene            complement(16529..17272)
                     /locus_tag="PLH85_11750"
     CDS             complement(16529..17272)
                     /locus_tag="PLH85_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNB65283.1"
                     /translation="MDRAELQQYLETVLEVGRFKDYCPNGLQVEGRGEIRHIVCGVTA
                     SRALLDAAVARGADAVLVHHGYFWRGEDGRITGLRKARLATLLCHDINLFAYHLPLDA
                     HPELGNNAQLGARLGLTAEGRFGDQALGWLGCTAPTTLAGLAARVADGLGRTPLVLGD
                     PARPVRRVGWCSGGAQGYFEAAIAAGVDCFISGEASEPTTHLARESGVAYLAAGHHAT
                     ERYGVQALGAHLAERFGLTAEFVEIDNPV"
     gene            17338..18513
                     /locus_tag="PLH85_11755"
     CDS             17338..18513
                     /locus_tag="PLH85_11755"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNB65284.1"
                     /translation="MHRLWLIFTQAVTVTVAALFVVGTLKPEWLGGRPGGASVIIQEA
                     VAPAAPSVAVAPAHAAASYADAARAALPAVVHIFTSKEVRGQRSPFANDPLFRHFFGD
                     RLDNQPQQRASGLGSGVIVSPEGYILTNNHVIEAADEIEVALQDGRKLPARIVGRDPE
                     SDLAVLRIKTDVRLPAITFGRTEALHVGDVVLAIGNPFGVGQTVTMGIVSALGRSHLG
                     INTFEDFIQTDAAINPGNSGGALVDASGHLVGINAAIYSRSGGSLGIGFAIPVSLARN
                     VMEQIIATGTVTRGWIGVEVQEITAELADSFGLPGTQGTLISGVMRGGPADRAGIRPG
                     DVLLDVAGHAVNDPQSMLEAVAALPPGNRAPLQIRRGREKVDVQVEIGRRPALARSE"
BASE COUNT         3238 a         6435 c         5833 g         3095 t
ORIGIN      
        1 acgcacttct gggacgtggt ggcgcgggtg taggggcgct tgtcgaagcc gaggaagacg
       61 tccttgaact ggttcatgcc cgcgttggtg aacagcaggg tcgggtcgtc gtggggcacc
      121 agcgagctgg aggcgacgat ctggtggccc ttggaggcga agaattcgag gaacttctga
      181 cggatttcgg cgcttttcat cggcggggag tctccggagg gcgtcgccgg gcgacgcacg
      241 ggggaacgcg ggtttctaaa cggcggattg tagcattcct ccccgcgagg cccggctggc
      301 tataatcccc tgcccggccc tgccgggaac aaccataaca tcgactccga gggagacaca
      361 gccatgggcc accgcctgtc caagatctac acccgcaccg gcgatgccgg caccaccggc
      421 cttggaaacg gcaaccgggt atccaagaac agcctgcgca tccacaccct gggcgaggtg
      481 gatgaagtca atgccgccgt cggcgtgctc ctctgcgagg acctgccgga ggacgtgcgc
      541 gtgctgctca ccaacgtgca gcacgacctc ttcgacctgg gcggcgaggt ctgcattccg
      601 ggcatggaga tgatcactgc aaagcaggtg cagtacctgg aagacgaact ggatcgccac
      661 aacgacgatc tggagccgct caaggacttc atccttcccg gcggcacccg cgccgcggcc
      721 ctctgccacc aggcccgcac cgtgtgccgg cgcgcggagc ggatgatcgt cgcgctggcc
      781 caggaagagg ccgtcaatga cgcgccgcgt cagtacctca accgactgtc ggacctgctc
      841 ttcgtgctgg gccgggtgct caacaaggcc ggcggacgcg gtgacgtgct ctggcagaag
      901 cgcaagaacg cctgaacctg caggacgcgt catcccgcgg gcgtcccgct ttcgaatccc
      961 ggccgacggt cgcgggttcg atcggcgccc tgctgcggac gatgcgccta ccagtccacc
     1021 ttgtccacga cacccaggga gcggccgaga aagcgctcac cgatggtctg gaaacgcaaa
     1081 ggaatgtcgg tgaccgtgta acgatatgtg ggcagccggt cgcccggatt ggcgataccc
     1141 aggtccgcga gggcgcgcgc ggcctgctcg gccaccgtga tggccgaatc cacgaggcgc
     1201 acctgcggcc ccaccacgtc ctgcagcagg ggcttgatga ggggatagtg ggtacagccg
     1261 agcaccagcg tatccacgtt ctcggccagc accgggcgca ggtattcctg ggcggtgagg
     1321 cgcgtcactt cgtgctccag ccagccctct tccaccagcg gcacgaacag cggacaggcc
     1381 tgggaataga cccttacgtt tgggtcaagg gcatgcatgc gccgggcgta ggcattgctg
     1441 ttgatggtgg tgggggtgcc gatcacgccg atggcgcggg agcgggattc cgccacggcg
     1501 gcccgcgcgc ccgcatcgat tacgtccagc accggcatat cgcccgcctt gtccttcacc
     1561 acgtgggacg ccaccgccgc catcgtgttg caggcgatga tcagcatctt gacgccctgg
     1621 tgcagcaggt ggtcggtgat ctgcccggtg aagtgttcga tggtggccac tgacttgacg
     1681 ccgtaaggca cccgcgcggt gtcgccgaaa tagacgatgc tttcgagggg caggcgctcc
     1741 atcagggcgc gtacgacggt gagcccgccc acgccggaat cgaagactcc gatgggcagc
     1801 gtgccggttt cagacatggc gggtgccgcg ggcgcggcgc aaacgaggga agcgggattt
     1861 tacgcgagtc accgccagat cggcggactg cgggacgggc ggatcagtgg tcgtcgtcga
     1921 ggcggccgat cagttcggac tcttcctcgg gcgtgaacgc gaacttgtag atggatttct
     1981 catcgggcaa ctcgcgccag cgcccgccga tctccttcag gcgggcttcg atggtggcgc
     2041 aggacagatg catcagcatg gccggggcat tgacacgggc gcagtgcttg acttcgccga
     2101 gctggtggaa ttcgaggagc ttctcgtaga aatcccggtg ccgcggattg atctcgatga
     2161 ggatgtcggt gcaccccttg acccggtacg agtagatgta gagcagattg aacagtgagg
     2221 cgatgagccg cttggaacgc gccgagggat ccactgcgaa gcgcccgaac tcgcacagct
     2281 tgcgcccctg ggaacgcagc cgggcggctt ccgcgccata ggtcttgtcg agggccagac
     2341 ccgccgggga atcgatattg accgtcaggg tcgcatagac gcgcccgaag agctccgcgt
     2401 tcatcgtgag ctggttcggc gcttcaaggg acccgagctt gccatacccg cgccacgcgt
     2461 agcgcttttc gatgagcaga tcggtctcac ggcggcggtc accgtcccgc atgagcattt
     2521 tgatgcggaa cttgttgctg ctgtccgacg gcgccgagac cttgggatcg tcctccgcga
     2581 tgcacagggg gtcgaagcgc cacgactcca tggcagtcac ttccgcgagc gtctgcggcg
     2641 gacgttcctc ctccggcgcc ggcttgcgcg acatcattgc caggacatcc cgcatctcag
     2701 cgcaccgtgc ctctcgagcg cccatgcaac atgctcccgg accagccagg attgatcatc
     2761 gcgtcgcgat tgaagcgcgc gaaccacctc tgcggatgag ggcgcgttgc ccagtccgac
     2821 ggcgaggtta cgcatccaac gttcatatcc aatacggaaa atgggactac ccgccatccg
     2881 ttcgttgaaa atctgtggcg tccaattaaa caactctaac agttttatgc gatcaagccc
     2941 atttcgaggc gtgaaatccg acacactcgc ctgtcctgca aagcggttcc aagggcatat
     3001 ccactggcag tcgtcgcagc catacacacg gttgccgatc atcgggcgat aaacctccgg
     3061 gatcgcaccg tgcagctcga tggtgagata ggagatgcac cgccgcgcat ccacccgata
     3121 gggtgccacg atcgcacccg tcgggcaggc gtcgatgcac gcccggcagg aaccgcaatg
     3181 accgctgcga ggcgcgtcca ccggcaactc cagatcagta tagatctctc ccagaaaaaa
     3241 gaatgacccc gcttcacgcg atagtaacag gctatgcttt ccccgccacc ctaaacctgc
     3301 cttttccgcc agtgccacct ccatcacggg cgcggaatcg gtgaacaccc ggtagccgtg
     3361 aggcgctgcg gcggagatcc ggtccgcaag cttctgcagc cgctggcgca gcaccttgtg
     3421 atagtcccgc cccagggcat accgtgaaac gtaggccaga ctacgatccg ccagcacctc
     3481 gcaggcatgg gccgcagccg ccgacagata atccatcctc accgagatga cgcgccgcgt
     3541 gcccgccacg agttccgccg gtcgcgtccg cttggtcccg tgtttctcca tataatccat
     3601 ggatccgtgg aaaccggcag ccaaccatgc agccagtccc tcttccacgc cctcaagcgc
     3661 gacatcggaa atgccgatgc cgccaaagcc cagttcttcc ccccacgccc ggatgcgcgt
     3721 cgcgagtgcg gcgagatcct cagccttccg tgagagtaca tcgtgccctg tgtccatgac
     3781 gcccatgata aacaccctgt gctttgcgcg cacctggcgg atgaagaggc caccctgacc
     3841 tacggtcgca ccctcgcatc cgtcatgccg gagcggctga tcatcctcct gcacggcgat
     3901 ctgggcgcgg gcaagacgac gcttacgcgc ggcctgctcc gcggactgga ctacaccggc
     3961 cgcgtcaaaa gtcccactta caccttggtt gaatcgtata aagattctag aattgtcgta
     4021 tatcactttg atttttatcg attcacccat gcagaagagt tcctcgaagc cggactggac
     4081 gagtattttc aagggcccgg cttacgcctt gtcgaatggc ccgagcgggc aggcccctat
     4141 ctgccccccg ctgatctggc gatccggcta cggattgcgg acgacggtgg ccgtacgctg
     4201 gatctccaag cgctgacccc ggagggacgg acatgcgtcg atcgcctctc accttccccg
     4261 cgcgcctgac ccgcccatcg attggcggaa acgcagcggg ctttacccgc cgccgcttca
     4321 tcggcgccgc cggcgcaagt ctggccctgc tggtgagtcg tcccggcact gccgcccaga
     4381 ccggcatcct ggccgtgcgg gtctggccgg cccaggacta cacccgcatc acgctggaaa
     4441 gccgcgaacc gctcacattt actcatcagc tcgtgcacgg accggaacgc ctggtcgtgg
     4501 atctggaggg catcgaactc aatggcgtgc tgcaatccct gccgaccaag atctccggcc
     4561 aggatccata catccagctg atccgcgccg gccgcaaccg gcctggcgta gtgcgcctcg
     4621 tcattgatct gaaggcggag atcgtgcctc aggtcttcac cctcaagccg gtgggcgaat
     4681 acggtcaccg cctggtgctg gatctctatc ccgtcgaacc ccctgatccc ctcctcgccc
     4741 tgctggagaa gctctcgccc gaagaggcgg cggcaggcgt gccgatgcct gcgcggcccg
     4801 aaccgtccag cgtgcggccg acgcgccccg ccgaacccgg catcgcccgc cggcaggagg
     4861 cggagccccc gccggtccgc ccgcagcctg cgccggaacc ccccgcggca cggcgcaagc
     4921 ctgccgaaga cgagggcgtg agccgcctcg tcacgatcgc cctcgaccct ggccacggcg
     4981 gcgaagaccc gggtgccgtg ggcgccgccg gcagcttcga gaagcacgtc accctgtcca
     5041 tcggccggcg cctcaaggcg cgcattgacg cggagcccaa catgcgggcc atgctcaccc
     5101 gagacgcgga ctatttcgtg ccgctgcagc aacgggtcag caaggcgcgg cgggtgcagg
     5161 cggacctgtt cgtgtccatc cacgccgacg ccttcatcag tccgacggca cggggcagct
     5221 ccgtcttcgt gctgtcggaa cggggcgcca ccagcactgc ggcgcgctgg ctggcccagc
     5281 gggaaaacgc cgcagacctg atcggcggcg tgaatctggg tgtcaaggac gagcacctcg
     5341 cccgcaccct gctggacctg tcccagaccg cctccatcaa cgacagcctc aaggtgggca
     5401 aggccgtgct cggcgaactg ggcaccatca atcgcctgca caagggcgag gtggaacagg
     5461 ccagtttcgc ggtgctgaaa gcaccggaca ttccgtccat cctggtggaa acggccttca
     5521 tctcgaatcc cgaggaggaa gcccgcctca acgacgaggc ctatcaggac cgcatggccg
     5581 acgcgatcct gcgcggtctc aagcgctatt tcgcgaagaa tccgcccacc gccaaatcca
     5641 agctggcgag cctgtaagtc gcccgtcggc gggcggcgtc gcggatctca gcggatgcac
     5701 accttgcgca cctgcttcag gtcggtgagg ccgcccagca ccttctcgat gccgtcctgg
     5761 cgcagggtgc gcatgccctc ctccagcgca actgagagca gattggcaac ccgcgcccgc
     5821 tcctggataa gtgccttgat gcggtcggtg cccaccatca gctcatgcag gccgagacgg
     5881 cccttgtacc cgtcggcgca gtgctcgcag cccaccggcg catacagcgt gaacgggccc
     5941 cggtccttgg cgtagcgctg acgccaggtc gccaggacct gctccttcgc gccgtgggcg
     6001 tcttcgatga aagccggcgt ctggtgcaga tcctcgcaat actcgtcgag cagatggcgg
     6061 atctcgtcct catccggcgt atagggccgc ttgcactttc cgcacagacg tttggccagg
     6121 cgctgggcca gcacgccgag cagggcgtcg gcgaagttga aggggtccat acccatgtcc
     6181 agcagacgca caatcgattc cggtgcgctg ttggtgtgca gggtgctgaa caccaggtga
     6241 ccggtgagcg acgcttccag accgatggag acggtctcct tgtcacgcat ttcgcccacc
     6301 atgatcacgt ccgggtcagc gcgcaggaag gaacgcatga cggtgggaaa atccagcccc
     6361 gccttgcgat tgacctgtac ctggcgcaaa cccttctggg tgatttccac cggatcctcg
     6421 gcggtccaga tcttcacgtc cggggtgttg atggaaccca ggatggaatg cagcgtggtg
     6481 gtcttgccgg aaccggtggg gccgcacacg aagaagatgc cgtagggctt ggagacggtg
     6541 tccttgaggc gccggtagtt gtcctccagc agcccgagct gatccagggg cagcggctcg
     6601 ctgttggcga gcagacgcat gaccacgtcc tccatgccgc ccgcggtggg cacggtggcg
     6661 acccgcagct cgatgtccag cggcccgtag cggcggaact tgatcttgcc gtcctggggc
     6721 ttgcgccgct cggagatgtc caggtcgcac atgatcttga tgcgcgtgac gatggggctg
     6781 cggtagctgg acggcacctc gatatagggc accagcgagc cgtccttgcg gaagcggatc
     6841 tgggtctttt ccttgccggg tcgcggttcg atgtggatgt cggaggcgcc ctgacggtgg
     6901 gcgtcgatga tgatgcggtt caccagcttg acgagctcgt tgtcggcggc cgcggagacg
     6961 tcctcggcgg tggaggtgcc ttcaccgtcg tcgtcggcca tgtcggagag cagctccgtc
     7021 accgacggat cctccgccgc gccgaagaac tggtccagcg tctgcacgaa ctcgtgatcg
     7081 gtcgtgaccc ggtagaccgg attcgccttg gggaagacgt tctgcaccac ccgtgaggcg
     7141 acggcctgct ccggatcgac agtgaggatc accataccct ccggagtgtc ctcgaggggc
     7201 agccactggg cctgctggac gtagtcccgc ttcaggttct tgaggagatc cagcggcttg
     7261 atgcgatcct ggcggtaggg ctcgtaaggc acgccgaaaa agcgctccag ggcggcgccc
     7321 acgtcggcca gcggaatacc gagatcgtcc agcagcaccc gctccatgga tttgcccacc
     7381 tcccgcgcct tgcgcagggc ttcccgcagt tcctgctcgg acagccgccc gccggacacc
     7441 agactctcga acttggactt gggctgggcc agggtatgcc tgcgctgggc gaaggcgatg
     7501 gcaagggtct gtccgagccc ctgcacacct tcctcgcaga ttgacgaaaa gggttcgccg
     7561 ctgcgcgtgt tgatgagctg gatcaccccc agcgtgtcgg cgccctcctg gcgcatgatg
     7621 ggcgccacga gcatctggtg gcagcgataa ccggagcggt cgtccacctc gcggcgaaac
     7681 tccatgcgct ccgacaccgc cttcagttcc cggtcgtcgt aggcgtcgtg gatgttgagc
     7741 atgcgcccgg tgagggccac ataacctgcc acgctgttct cggcgatggg cagacggatc
     7801 gtgcgcaccg cctgcagccc ggtcttgacc cgagaggaga tggtggtgcg attgtcgtcc
     7861 accagataga tggtcagccg gtcggcggcg aacagggtgc agatctccga ggacagctcg
     7921 aagatgatct cgtcgatgtt ctcggtggca tgaattcggt tggtcaccgc ctgcaagccc
     7981 ttgaaaaaag ccaacctgct cgctacgtca ctgccacgca ccatgccgct cgtcatcccg
     8041 tcgtaatctg caagaacagc gcccgcccgg gtgggcgccg aatgtaaggg cgcttacagc
     8101 gcgaattcca gtccgttgta cagcgcccgc ggctcgaagc gaccgcaata gctgacgatc
     8161 tcgtccaggg tggtctgagc acggctgggc ttaaggtggg tgatccacac ctcgggattg
     8221 accgtgaggc gcccgaggaa cagcgccagc agactcgggc acaggtgccg cgacgccagc
     8281 gccagcccct gcagcgcgtc ggggaaggcg gtctccacga tcaggtagcg cagatcgggg
     8341 atctcgttga tcgccgcaat gagcggctcg gaataggtcg tgtcacccga gaacaccaga
     8401 ctgccatgtg cacaggcaag ccggtaggcc accgccggaa cggtatgcag cgcgggcagc
     8461 gcagtgatct cgcgcccacc gtcgagcgcg acggtttcgc ccacgtggat ctcgtgaaag
     8521 cgcagaaagg ggttcccccg atcaggaatc gacgaaaaat ccggccagat caaccagttg
     8581 aagatatggg agcgcaggat gcgcagggtc tcgggtgagc catgcacgac gatgggagtc
     8641 gaccgggcct cgcccaccgt atccacgagg agcgggatca tcgcgatgtg atccagatgg
     8701 gcgtgcgtga cgaagacatg atcgatcttg aggagctcgt cgaactccag atcaccgaca
     8761 ccggttccgg catcgatcag cacatcctga tcgacgagca gggacgtcgt gcgctgactg
     8821 cgcccgccga tgccaccact acaccccaga acctgcactt tcatgtcgtc agcccgatcc
     8881 ctcgcagaaa tggcgacagg gtatgcacac cggctcgcca ggaacccccg ccgccaatat
     8941 gtcaggatac ggattgccgc cagactggcg cgttagaaat ttcacgaagg aatcgacggg
     9001 tcgccggagg gaggggactc agcgctcggt gggctgggag aagaattcca tcttgacgcc
     9061 cgcgatctcg atgatgtcgt gatcgttgag gcgctgggcc tgcggatcga gggcacggcc
     9121 attgaccacc ggaaagctcg ccccttccac atgggtgatg aagtaaccgt gggggcgccg
     9181 ggtgatcacg gccacctgaa gtcccggctt gcccagggtg gtcagggact tggagagttc
     9241 gagggtccgg ccggcgtggg aaccgctcag gatctggatc atgcccatgg cggccggcgc
     9301 cggttgtggc gccgcgctcg gcggaggttc cgtcgccggg gccggggccg ctccgcgtga
     9361 cgcatcggtg cgcgtcaggt ccgtggcgaa ggcaacggaa tccgtcatgt ccatcggcgc
     9421 cgccaccgtg ctgtcggtga agaacttgag gcggtacttg cccagctcga cgacatcatt
     9481 gttctgcaac acgtgcttct tgaccggctg gccgttcacg taggtgccgt tggtgctgtt
     9541 gagatcctcg agaaaggcgt cattcaggat ggtgacaatg gctgcatgct cgccgctgat
     9601 ggccagattg tcgatctgga tatcgttatg gggcttccgg ccaatggtgg tgcgctcctt
     9661 ttcgagcacc atctccttga gaaccagacc gtccatgctc aaaatcaacc tgggcatcgc
     9721 atttcctatc agaaacaagc cttacgctat taccgagacg ggcagaccac cggaccgcag
     9781 ggatacgacc accacggata cattatcggt ccccccctga tcgttggcca gatcgacgag
     9841 atgctccgcc gcaaggcgtg ggttggcacc cagcgtatcc agcacatgga agatttctct
     9901 gtcgggcagc atgtccgtca ggccatccga acacagcagg aagacatcct ccgcgcacac
     9961 ctgcgtgacg atcaggtcgg gctggacctg cacatcaacg ccgaccgcgc gcgtcaataa
    10021 gcctcgcaac gcgggcgccc cgtcggattc cgcctcaatc atgcccgcgt cgcactgctc
    10081 ccgcaagacg ctgtggtccc gtgtcaggca ttcgaggctt tcacctcgca ggcgatacag
    10141 gcgcgaatct ccgacatgac aggcgatcat ctgaccgtcc gtccggaaac acacggcgac
    10201 cagcgtggtc cccatgctgc gcagcagaac atcctggcag gcggtgtcga agacgacctg
    10261 attggccgcc gcaacagccg cacgcatgca ggtttcgtcg atcgtgccgg gcggacgatc
    10321 ttccacttcc gccagccagg ccgccaccgc cagccgggac gccagcgcac cgcccgcatg
    10381 cccgcccatc ccgtcggcca gcagcgcgat gccgcgcgat gcgtcgcaat agacctgatc
    10441 ctcgttgcgc acccgcacgc gccccgtgtc ggagagcatg gccatcctga ggggaagata
    10501 ggcgtcggcc acgacgatct gctccgcgcc gggcgcctag agcgcctgct ccgaagaacg
    10561 gccggcgcgc atcgccagca cgcgcgccac gccgatcacg ccaagcgccc cgagcgcgcc
    10621 cgccagcatc cgggcccagc cgggcaggcc gtccacccag ggtcgcaagc ccggatccga
    10681 cagcatcatg ctgcccgcca cccaaccgag cagcgcagcg ccgaggctca cgatgacagg
    10741 aaaacgctcc atgctcttca tgatgagctg gctcgaccag acgatcaccg ggatgctgaa
    10801 ggccagcccg agccccagca ggacgaggct gtcacctgcg gcccccgcca caccgatcac
    10861 attgtccaca ctcatgacga agtcggccac gatgatggtc cgcacggcac cgctcacgct
    10921 ggtggccgcg tcgatctcgt ggccgccgtc atcgtccggg agcagcagct tcacgccgat
    10981 ccacagcagc aggagcccgc cgagcagctt gagccacgga tagcccagca gacttgccgc
    11041 aaacgcggtc aggacgaccc gcagggccac cgcaccgacc acgccccaca ggacaccgat
    11101 gcggcgctgg gcaggcgaca gacgtcggca ggcgagcgcg atcacgaccg cgttgtcgcc
    11161 ggacagcacc agatcgatcg cgacgatctg gacaaacccc gcccagaagg cggcggtcag
    11221 gaattcgggg gttgaaagca tggattgcag gaatccggaa gcgtcattca cgacgcattt
    11281 tatcgcgcct cgcaaggatc gcgcctacaa atgaaaaccg cgcccgggca gaaccccggg
    11341 cgcggcgatc ggttcctgaa cgaaccggat tacagcaggg ccttgagcag cttgcccatt
    11401 tcggacgggt tgcgcgtcac cgtgaagcca cactcttcca tgatggcgag cttggcatcg
    11461 gcggtgtcgg cgccgccgga gatcagcgca ccggcgtggc ccatgcgctt gccggccggc
    11521 gcggtgacac cggcgatgaa gccgacgatg ggcttcttca tgttggcctt gcaccactgg
    11581 gcggcttcag cttcgtccgg gccaccgatt tcgccgatca tgatgacggc gtcggtatcc
    11641 ggatcgtcat tgaacatccg catgacgtcg atgtgcttca gaccgttgat cgggtcgcca
    11701 ccgatgccga ccgcggaaga ctggcccaga ccgatttcgg tcagctgggc gacggcttcg
    11761 taggtcaggg tgccggagcg ggacaccacg ccgatgcggc ccttgcggtg gatgtgaccc
    11821 ggcatgatgc cgatcttgat ctcttcgggc gtgatgaggc ccgggcagtt cggccccagc
    11881 agcagggtct tcttgccacc ggccgcttcc ttggccttca tcttgttgcg cacttccagc
    11941 atgtcacgga cgggaatgcc ttcggtgatg cagatggcca gatccaggtc agcctcgacg
    12001 gcttcccaga tggcagccgc ggcgcccgcg ggcggcacgt agatcacgga cacggtggcg
    12061 ccggtttcgg cggccgcttc cttcacggtc ccgaagatgg gcacgtcgag gatcttctcg
    12121 ccggccttct tggggttcac accggccacg aagcagttct tgccgttcgc gtattcgatg
    12181 cacttctcgg tgtggaactg accggtcttg ccggtgatgc cctgggtgat gactttggtg
    12241 tctttgttga tcaggatgga cattcacgta ctccttactg aaccgcagcg acgatcttgg
    12301 tcgcagcttc ggccatggtg tcggcggaga tgatcgggag gcccgaatcg cgcaggatct
    12361 gcttgccgat ctcttcgttg gtgcccttca tgcggacgac cagcggaacc gacagatgca
    12421 cttccttggc cgcggtgacc acgccggtgg cgatggtgtc acagcgcatg atgccgccga
    12481 agatgttgac gagaatgccc ttgaccttgg ggttcttgag catgatcttg aaggcttcgg
    12541 tcaccttctc ggtggtggca ccgccgccca cgtcgaggaa gttggccggc tcggcaccga
    12601 acagcttgat ggtgtccatg gtggccatgg ccagaccggc gccgttcacc agacagccga
    12661 tgttgccgtc cagggagatg taggccagat cgaacttgga ggcttcgatt tcgtccgcgt
    12721 cttcttcgtc cagatcgcgc atctcgacga tctcggggtg acggtagagg gcgttggagt
    12781 cgaagttgaa cttggcatcc agcgccttga cggtgccgtc gccttcgtgg atcagcgggt
    12841 tgatttccgc cagcgacgcg tcggtgtcca tgtagcaggt gtacaggttc ttgagcgtgg
    12901 acacggcctg ggcgatggag ccttcgggga tcccgatgcc cttggcgagg gtcagcgcct
    12961 gctcgtcggt gaggcccacg gccggatcga cgaacacctt gatgatcttc tcgggggtct
    13021 tgtgggcgac ttcctcgatg tccatgccac cttcgctgga cgccatcatg gcgaccttct
    13081 gggtggcacg gtcggtcagc gcagcaacgt agtattcgtg cttgatgtcg gcgccttcct
    13141 cgatcagcag gttacgcacc ttctggcctt ccgggccggt ctggtgggtg atgagctgca
    13201 tgccgaggat ctggccggca tactggcgca cttcgtccag ggacttggcg actttcacgc
    13261 caccgccctt gccgcgaccg cccgcgtgga tctgtgcctt gacgacccag atcttgccgc
    13321 ccagggtttc agctgccttg accgcgtcgt ccacggtcgt gcagtggata ccgcgcggaa
    13381 ccgtaacgcc gaacttcttc aggatttgct tgccctgata ttcgtgaatt ttcatggttg
    13441 cccttcgatc gaagtcttgg cctttgcagg ccgctcacgc ttggttgatg ggactcattc
    13501 ggggcctgcg ctgcaccccg cgacccgtac gccgttctcc ctcgtccgac gtacgcaccg
    13561 ctcgagctct tcgctcaccg gaccgacagg gccgcggtgc gtgaaaaacc atcctttcct
    13621 gccggcgccg tgcctggatg tggccctttg ccggattgtg gactccataa ttatataacg
    13681 aaaccggagc aggttccgtc gccggacagg caaactcagt cgccgctgcg ataccagcgc
    13741 gggtagtagc ggagcacggt ttgggtggtg gtttcgaggg cgtggcagcg atcgagctgg
    13801 aagggcttgc cgtccgtgtc ctcggtgtcg cggcggaagg tggccagggt ctggatcgcc
    13861 gccgtcggca gggacagcag cagctcgctc agatgggagc agccgcgcac gtcggcgaac
    13921 atctccccca ccgtcttgcg gaatcctttg accagattca gccccaccag ctgccggtag
    13981 gccggcgtga tggtctcgca gccgcccgga tagggcaccc gcgccgacac cgcgcaggcg
    14041 cccacgatat tgaactgggt gtcgatggtg acccgcagga acaggtcgtg gatcggctca
    14101 ccggccttgc gcaggcctga cgcgatcacg tagtcctgat ccttcacgtc ggtgaggcgg
    14161 gcatcgagat cgagcagacc gtcctcgcgg aggaagcctt cgatctcgat acggcgggca
    14221 tggacgcggc gccgggccgc ggaggacggg ggcagggaca tgacagcgga acaatgaagc
    14281 aggtcggcct tcagaaaaca caaaaagtga cgcaaaagga ctcagtcgaa aagtgaacgt
    14341 tgctcacaac gcccgcgacg gcccggaaag ttctttagaa tacttgagca cctgcccctg
    14401 cgcgacctta attttgcgcc gcacaatcat gaagaggccc aaggaggctc tccagccaga
    14461 tttcctgatg aaccgcgtgc cccgaacttc cctgcttgtc attgccgttc tcgcagccct
    14521 gcccgtggcg cccgtgcgcg gcgccgcgct cggcgaactg tcggcccgct cggccctggg
    14581 cgcgcccctg aatgccgaga tccgcatcac cccggcgccg ggcgaacaga tcgacggtca
    14641 gtgcatccgc atcgtccgcg ggcagaccga cgatctgccg tggctggccg gcgcccgcgt
    14701 gcgccttgaa ggcgagcgcc tgatcatcac cacgcgcgac cccgtcaatc atccggccgc
    14761 catgctgggc gtacacatcg gctgcggtat cgaattgcgt cgcgactacg cgctgctgct
    14821 gcagcccccc ctcgaaagcc ccgccgtgac cgccgcggtc gtcacgcccg atgcaccggc
    14881 cccggcgcgc gcgccgtcga cgcctccggc cgccgcccgt ccgagacctg tcgggcaggt
    14941 catcgtgctg tccgaagaca ccccgctcgc gcgcctggcc gatgaacgcg ccggcccctc
    15001 acccgcacgg cgccagcgct tcatcgagcg actcgtcatg gccaatccgg atgtcttccc
    15061 gagcggccgc gccgatgagg gcctcgtcat cgcggccggc acgcgcctca agacgacgcc
    15121 tcccgccctg cccgcgcacc acgcagacac gcctccgcgg cgcaccgaca cggtcgcacg
    15181 tgcccaggcc gctccggcag ctccgacaag ccctgctcag gcgacacgcc cgagcactcg
    15241 ccgcgaaccc gcaccgcgcc aggaccgcct ggtcgtcgcc ggcacggccg gcgcggacga
    15301 catgcccctg cgcctgagcc tgcagctggc caatccgcca cgggacgacg cccccggcga
    15361 cgccgttcgc ggtccgatcc gccaggagca gcgcaccctc accgagatcg acgaccgcat
    15421 cacggcccag ctcgaactca acgagaagat caagcgcctt gaggaatacc aggagctcct
    15481 caaacgcaag atcgccgacc tcgacgccgg cccgccgggt caggccgcgc ccactgccgc
    15541 cccgcccccg gcgcctgccc cggctgcagc cccgcgggtc agcacgccgg cggccgtcgc
    15601 gccaccggaa tctgccgccg aaggacttcc cgcctgggcg ctgcccgcgg gggccggcct
    15661 tgtcaccctc ctgctgggcg gcggcgccct cgtgtggtgg cggcgccgca atctggtcgt
    15721 gcccgaggat tccctcgaac cgatctccgt cggcgccgaa tccaccaccg ggctgcacgc
    15781 cgaaaccccg ccggtcttcg tcaccacgat ggggccgccg tcgatcctga taccgagccc
    15841 ggccgccgcg ccggcccccc agtccgccat gccggtgcgg gaaatgcctg ccctcgacat
    15901 cgacctggcc cccaagggcg actggtccga gcccaccttc gcccccaccc attccgtgcc
    15961 cttcgacgag gccgtggacg aacaggattc cgcgctggag ctggccgaga tcatgatgtc
    16021 cttcggccgc acccagggcg ccgccgaaac ccttgccgac tacatccgca acaacccgcg
    16081 gcaggccgtc aagccatgga tcaagctgct ggaggtctat cacctggccg gcatgcgggc
    16141 cgagttcgaa gccctcacgc ggcagctcaa caagaccttc aacgtcaaga tggtgagctg
    16201 gcacgactac cgcgcgcccg aaacagcgcc ggattccgtc gagcagctgc cccacatcgt
    16261 gcgccgcctg gaagaaacct ggaacaccgc cgacgcccag gcctacatcc accagatcct
    16321 gcgtgacaac cgggacggca cgcggcaggg cttcccgctg ggcgtggtgg acgacctcct
    16381 catgctgctg gcggtcctgg acgaccatct cggtccctac cgccaagcgg cggaaacgcc
    16441 cgacaacgca cccgcccccg ccgataccca caaggcggcc gcctgaaacc tggcgggcgc
    16501 caccgcgccg cccgtcgggc cgcccgcctc acaccgggtt gtcgatctcc acgaactctg
    16561 ccgtcagccc gaagcgttcc gccagatggg cgcccagcgc ctgcacgccg tagcgctccg
    16621 tggcgtgatg gccggccgcc agataggcca cgccggactc ccgcgccaga tgcgtggttg
    16681 gctccgaggc ctcgccggag atgaagcaat ccacccccgc cgcgatggcc gcctcaaagt
    16741 aaccctgggc accgccgctg caccacccca cgcgccgcac cggccgcgcc gggtcgccca
    16801 gcaccagcgg cgtgcgtccc agcccgtccg ccacccgcgc cgccagcccc gccagcgtcg
    16861 tgggtgcggt gcagcccagc caaccgagcg cctgatcgcc aaaacgcccc tctgccgtca
    16921 ggcccaggcg cgcgcccagc tgcgcgttgt tgcccagttc cggatgggcg tccagcggca
    16981 ggtgataggc gaacaaattg atgtcgtggc acagcagcgt ggccagacgc gccttgcgca
    17041 ggccggtgat gcgcccgtcc tcgccgcgcc agaagtagcc gtgatgcacc agcaccgcgt
    17101 cggcgccccg cgccaccgcc gcatccagca atgcacgact ggccgtcacc ccgcagacga
    17161 tatggcgaat ctccccgcga ccttccacct gcaggccgtt tgggcaataa tccttgaagc
    17221 ggcccacctc cagcacagtc tccagatact gctgcaattc ggcccgatcc attccgtgtc
    17281 ctttcaatcc cttggcatga ttccgcagcg ctgcggcgcg cggcccccga ttctacgatg
    17341 catcgtctct ggctgatctt tacccaggct gtcaccgtca ccgtggccgc gctcttcgtc
    17401 gtcggcaccc tcaagcccga atggctgggc ggacggcccg gcggtgcctc ggtgatcatc
    17461 caggaagcgg tcgcccccgc cgccccctcg gtcgccgtcg ccccggccca cgccgccgcc
    17521 tcctacgccg acgccgcccg ggccgccctg cccgcggtgg tgcacatctt caccagcaag
    17581 gaagtgcgcg gccagcgcag ccccttcgcc aacgatcccc tgttccgcca cttctttggc
    17641 gaccgcctgg acaaccagcc ccagcagcgc gcctccggcc tcggctccgg cgtcatcgtc
    17701 tcgccggaag gctacatcct caccaacaac cacgtcatcg aagccgccga cgagatcgag
    17761 gtggccctgc aggacggccg caagctgccc gcgcgcatcg tcggccgcga cccggaatcc
    17821 gatctggccg tgctgcgcat caagaccgac gtgcgcctgc ccgccatcac cttcggccgc
    17881 accgaagccc tgcatgtggg cgacgtggtc ctcgccatcg gcaacccctt cggcgtcggc
    17941 cagaccgtca ccatgggcat cgtctccgcc ctcgggcgct cccacctggg catcaacacc
    18001 ttcgaagact tcatccagac cgacgccgcc atcaaccccg gcaactccgg cggcgccctg
    18061 gtggatgcca gcggccacct ggtcggcatc aacgccgcca tctactcccg cagcggcggc
    18121 tccctcggca tcggcttcgc aatccccgtc tccctcgccc gcaacgtgat ggagcagatc
    18181 atcgccaccg gcaccgtcac ccgcggctgg atcggcgtcg aagtgcagga aatcaccgcg
    18241 gaactggccg actccttcgg cctgcccggc acccagggca ccctcatctc cggcgtcatg
    18301 cgcggcggcc ccgccgaccg cgccggcatc cgccccggcg acgtcctcct cgacgtagcc
    18361 ggccacgccg tcaacgaccc ccaatccatg ctcgaagccg tcgccgccct cccccccggc
    18421 aaccgcgccc ccctccagat ccgccgcggg cgcgagaagg tggacgtcca ggtcgaaatc
    18481 ggccgccgcc ccgccctggc ccggtcggaa taagccttcg ggcaccgggc gcgctccctc
    18541 ccctgcgcac cgcgcccttc ccggcgcact ccgcccaaca ctgggcacgt caaagcgctt
    18601 c
//
LOCUS       DAOQMM010000071        18467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1121_length_18467_cov_56.784543, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000071 DAOQMM010000000
VERSION     DAOQMM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18467
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_18467_cov_56.784543"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="PLH85_11760"
     CDS             <1..84
                     /locus_tag="PLH85_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNB65285.1"
                     /translation="LAGLIDAAFLIGVGIAMMFAFANPFKI"
     gene            119..592
                     /locus_tag="PLH85_11765"
     CDS             119..592
                     /locus_tag="PLH85_11765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNB65286.1"
                     /translation="MNLNATLVAQLVVFFILAWFTMKFVWPPIVKALDERAKKIADGL
                     AAADKAKSDLAHAEKKVVEELRKARESATDVRAAAEKQAASFLDEARAEAARIIAQAR
                     EAAEAEAGVAAQRAKEALRDQVAHLAVAGAEKILRKEINAQAHAELLANLKQELQ"
     gene            596..1132
                     /locus_tag="PLH85_11770"
     CDS             596..1132
                     /locus_tag="PLH85_11770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNB65287.1"
                     /translation="MAENVTIARPYAEAAFQLASAGNALGPWSEALDRLAGIAADPQM
                     RDCISDPKLQPQQLAGLFLDVAGSGLTAEQQNYVRVLVDNERLQVLPEIRDLFVALKN
                     EHEGVKEANIVSAFPMDEATLKALVADLEARFKARLNVSVSVDPELIGGVKIAVGDEV
                     IDASVRGKLANMAAALKN"
     gene            1144..2682
                     /gene="atpA"
                     /locus_tag="PLH85_11775"
     CDS             1144..2682
                     /gene="atpA"
                     /locus_tag="PLH85_11775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNB65288.1"
                     /translation="MQLNPSEISDLIKSRIQNLQLSANARTEGTVVSVTDGICRIHGL
                     SDAMQGEMLEFPGNVFGLAMNLERDSVGAVVLGEYEGISEGDTVKCTGRILEVPVGPE
                     LIGRVVNSLGQPIDGKGPINAKLSEPIEKVAPGVIWRKSVSQPVQTGLKSVDSMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGENMICIYVAVGQKASTIANVVRKLEENGAM
                     EYTIVVAATASESAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSAEYVEKYTNGEVKGKTGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLERGRLVTELMKQPQYSPLKVSEMAITLFA
                     VDKGAFDDVEVKRALACEAAMHQYVKTKAPDLINKIETTKDLDADASKQLADVIAEFK
                     KSWA"
     gene            2704..3573
                     /gene="atpG"
                     /locus_tag="PLH85_11780"
     CDS             2704..3573
                     /gene="atpG"
                     /locus_tag="PLH85_11780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNB65289.1"
                     /translation="MASGKEIRNKIKSVQNTKKITKAMEMVAASKMRKAQDRMRAARP
                     YAEKIRRLAANLSAANITDYKHPFLTKKDDAKRVGLIVVTTDKGLCGGLNTNTLRVAL
                     SALKSWEGNGVTDIRVTCIGNKGLGFMQRLGAKVISNVTQLGDTPHLEKLIGPVKVMI
                     DAFQNGELDEVHIAYSRFINTMKQEPQLEQLLPLTGERLGTPEGTWDYLYEPDAQVVI
                     DELLVRYVEALVYQGVAENMASEQSARMVAMKAASDNAGNVISELQLVYNKTRQAAIT
                     KELSEIVGGAAAV"
     gene            3601..4977
                     /gene="atpD"
                     /locus_tag="PLH85_11785"
     CDS             3601..4977
                     /gene="atpD"
                     /locus_tag="PLH85_11785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNB65290.1"
                     /translation="MSQGTIVQCIGAVVDVQFPRDAMPKVYDALTLEGAELVLEVQQQ
                     LGDGVVRTIALGSSDGLRRGMVVSNTGKGIAVPVGHGTLGRIMDVLGRPIDEAGPIEC
                     DERRVIHQKAPKFDELSPSVDLLETGIKVIDLICPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKQHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVFGQMNEPPGNRLRV
                     ALTGLTMAERFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGR
                     LQERITSTKVGSITSIQAVYVPADDLTDPSPATTFLHLDSTVVLSRDIAALGIYPAVD
                     PLDSTSRQLDPLVVGEEHYNTARGVQQTLQRYKELRDIIAILGMDELAPEDKLLVARA
                     RKIQRFLSQPFFVAEVFTGSPGKYVPLKETIKGFKTILSGEVDHLPEQAFYMVGPIEE
                     VFEKAKTL"
     gene            5041..5466
                     /locus_tag="PLH85_11790"
     CDS             5041..5466
                     /locus_tag="PLH85_11790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNB65291.1"
                     /translation="MAMTIHVDIVSAEEQIFSGLAEFVVLPGEAGELGIMPGHMPLMT
                     RIKPGAVRVKVQNQADEELVFVAGGLLEVQPNLVTVLADTAIRGKDLDEAKALEAKRR
                     AEEAMQNQDSALEYAKAQSELASALAQIAAIQKLRKRSH"
     gene            complement(5746..6324)
                     /locus_tag="PLH85_11795"
     CDS             complement(5746..6324)
                     /locus_tag="PLH85_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNB65292.1"
                     /translation="MLTSTVIAALNHLLTDAPWARDRLKPFAGRTAVIQVAPASLCVA
                     VEGDGFFSDSATADTPDVRLELPVSSLPKAVGGMDALMGDIRITGNADFADALGFVLR
                     HLRWDGEEALAKRVGDIAAHRAINGVRDATRWHLDAARNLTENFVEYFTEERPLILAR
                     PQFDPFATEMAALRDDLARLDKRLSRLEAARR"
     gene            complement(6389..7276)
                     /locus_tag="PLH85_11800"
     CDS             complement(6389..7276)
                     /locus_tag="PLH85_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNB65293.1"
                     /translation="MKKLAVAALMAVATLTTVVPDAEAARLGGGKSFGMQRNDTIVRR
                     EAAPAPQVPPRTAAAPAQSAPQAGAPAPQMPQKRSWLGPLAGLAAGIGLAALLSHFGL
                     GEQAASFMLILLGVMAVMMLFRLLFRRGASQPTPQYAGMPSTPAPETRHAASAPIALP
                     TSGAPVADTEALPSGFDADAFVREAKLNFIRLQAANDAGNLDDLREFTSPEMFAEIRL
                     QYDERHGKPQRTDIVNLNAAVLDVATEGSRHVVSTRFYGMLREEDDAAPTAFDEVWHL
                     VKPVDGSRGWMVAGIQQRH"
     gene            complement(7304..8041)
                     /gene="ubiE"
                     /locus_tag="PLH85_11805"
     CDS             complement(7304..8041)
                     /gene="ubiE"
                     /locus_tag="PLH85_11805"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNB65294.1"
                     /translation="MNDKTTHFGFQKVAEAEKAKKVAGVFSSVASSYDVMNDLMSFGL
                     HRLWKAFTVQIAGLRAGDRVLDVAGGTADLSLAFAKKVGPTGQVWLTDINHAMLSRGR
                     DRVVDNGFLLPVAQCDAEKLPFPDDYFDCVTVAFGLRNMTHKDQAIAEMRRVLRPGGR
                     LLVLEFSKVWKPLAPAYDFYSFKLLPLMGEKVAKDGESYRYLAESIRMHPDQESLKDL
                     MEQAGLGRVEYFNMTAGVVALHRGYKL"
     gene            complement(8103..8528)
                     /locus_tag="PLH85_11810"
     CDS             complement(8103..8528)
                     /locus_tag="PLH85_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNB65295.1"
                     /translation="MAGLQKGDPLPTEIKLHQRSRILEITFDDGQRFELSYEFLRVHS
                     PSAEVRGHGPGEEVLQLGKRDVEIVNVEPVGNYAIRPIFSDGHDSGLYSWDYLYEIGE
                     RRDELWQTYLTRLAVEGGSRDPAELPPAAPKAGGCGHKH"
     gene            8693..9403
                     /gene="phoB"
                     /locus_tag="PLH85_11815"
     CDS             8693..9403
                     /gene="phoB"
                     /locus_tag="PLH85_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNB65296.1"
                     /translation="MPATILLVEDEPAIQELIAANLLRAGHQVVRAGDAETAQRIVRD
                     ALPDLVLLDWMLPGMSGIDFARRLRADERTRGIPLIMLTARGEEQDKVLGLEVGADDY
                     VTKPFSPRELMARIKAVLRRRAPESTEDPVDLGGLRLDPATHRLTAGSTPVSLGPTEF
                     RLLHFLMTHPERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRCALEPTHHDGLVQTV
                     RGSGYRFSTQTESQPAVR"
     gene            9482..10771
                     /gene="phoR"
                     /locus_tag="PLH85_11820"
     CDS             9482..10771
                     /gene="phoR"
                     /locus_tag="PLH85_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNB65297.1"
                     /translation="MLVVLAVIFVLGLGVGLFFGPVPGLTVFSVALLAAGVYHIGNLF
                     KLVKWARQPVGTPLPRAVGLWDFVYADLNRLIRQAQDQRAKLTAALARFREASQAMPH
                     GMMFLTDTNFIEFANRQAELHFGIDNARDKGAPVINLVRQPDFVRYMNEGRFDEPLTF
                     SQFRPQSRNLMVHVVPFGEGMRLLMSEDITQGERLETMRRDFVANVSHEMRTPLTVVR
                     GFLETVLDGLDDLDKDDIRHYLAMAGEQAGRMGRLIEDLLALSALETGAPMPHEEPVD
                     IARMLKDVEQEARAVSAERHSIVVDPPAPGTERILGNSKELHSAFANLAVNAVRYTPA
                     GGHIHIAWRLRGAVVEYSVEDDGIGIAPEHIPRLTERFYRVDRGRSRETGGTGLGLAI
                     VKHVLSRHQADLHIDSEPGRGSRFSACFPARRLAAAE"
     gene            complement(10777..12387)
                     /locus_tag="PLH85_11825"
     CDS             complement(10777..12387)
                     /locus_tag="PLH85_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65298.1"
                     /translation="MSDNAVTFTELRSVEQYRKLLRNLHTLNASESHATLVRMLEGLL
                     ASPLGPEEQLEVLESARQTITFVQHEMSSRYAAHPLPPDSAEDATLRHVVRLWTALST
                     AYARLGDSGANDAEFRAQLPLLAQRRIQYLGQIIVEHYRAHRAEPPGAWRAVHAAFLE
                     AERAGITHSRVIDPLNNTWKAQSPNEAYIALLLIDLTNPYGRNQRELTWICRWAQRFA
                     PYCIMQTTWDKDDTRTYGLDPSEDHGLRPIALIGNRPGMRRFDGHRLAAQIKAMLAEF
                     KQGTTPASLGLGDDCAQPACARLLVSLYRPWGMSAAGRRFPRRPTRGVNRICGEWDAI
                     GMLVSGKEFVPPNRRQMMGSRYDMNMLTFGARADDDIEDIRDPEAEVQRRGHAIDEWQ
                     TVDHSVSGFRLMRRQPAQRIEHHQLVGIKPEDGGPYLLGQVSWLMFEADGTLMAGIHL
                     LSGLPTVVAARPVSVNVSAHEPYSQAFMLPAIPAMRAQATIVMPSGWFQANRVLDISP
                     GEIRQIRLTSLIARGTNFDQATYEVIGG"
     gene            complement(12488..12931)
                     /locus_tag="PLH85_11830"
     CDS             complement(12488..12931)
                     /locus_tag="PLH85_11830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNB65299.1"
                     /translation="MPHSAPKDCPLCFPERETVLWQDASCRVIRVDDADYPGYCRLIW
                     NTHVAEMSDLSTADQRHCFNILMAVEVALRECYRPDKINLASFGNVVPHLHWHLIPRY
                     TEDRHFPHPVWGQAQRRGGVSPAPAVDDAHLAGALRNAIAERLSG"
     gene            complement(13039..13857)
                     /locus_tag="PLH85_11835"
     CDS             complement(13039..13857)
                     /locus_tag="PLH85_11835"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNB65300.1"
                     /translation="MCQLLGMNCNTPTDICFSFAGFQARGGLTDVHGDGWGIAFFEGR
                     GVRVFLDPAASCRSPVAELVRQYPIRSLNVIAHIRKATRGIVKLENTHPFQRELWGRY
                     WVFAHNGHLGEFDPPLRGDFRPVGQTDSERAFCFMLESLRTRFPDGEPARPALFAAIR
                     ELATAVSAHGEFNFLLSNGTCLFAHCASRLSYIVRQAPFAQAHLKDQDITVDFSELTT
                     PGDRVAVVATTPLTDDEVWTPIAPGSLMLFVDGAPEAAGEASTRAFIRTESVVA"
     gene            13979..14785
                     /locus_tag="PLH85_11840"
     CDS             13979..14785
                     /locus_tag="PLH85_11840"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNB65301.1"
                     /translation="MTFDTLNDHSSAIEAVGRLLSTARRVLFITGAGMSADSGLPTYR
                     GVGGLYEGTVTDIGLPIEKALSGAVFASRPDITWRYLAQIESACRSARPNAGHYAIAR
                     LEAQRGGVTVLTQNIDGFHRGAGSRDVIEIHGTLHRLRCVSCGRAREVADYAGLSIPP
                     ECPHCGGVLRPDVVLFGENLPEAAVQRFEAALAEGPDLVFSVGTGSAFPYIAGPVVWA
                     AQQGIPTVEINPGTTPVSDIVSMRLPLRAGEALPALLDAAGLGLEGIRPD"
     gene            14844..18206
                     /locus_tag="PLH85_11845"
     CDS             14844..18206
                     /locus_tag="PLH85_11845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB65302.1"
                     /translation="MDLQRLVAQIDDALLAELFSANALAKARAYVGRVQHIEAAGHQI
                     QALVQGSDAAPYHVTVRIERREFFGKSSIELATRCTCPVGNRCKHAAALVLAARRAGA
                     LVDKPRAEILNWARSLGERLAKADKNRRKAAASEAIFYLCSAAQYGDDVEFGLLKGRV
                     GADGHPSGRASAWYNYDQALLKPPSFVRDEDLQVFRLLREVVRHGAGYGRPRLRGTEG
                     LALFEAALATGRAYLELDDNGRIQPLQAAAPRPGALIWAPDEAGLRPRVAVTPAAPLV
                     IRTEPLAWIDSATGEAGRVNIDGGGALLEVLALPPLSTTEIPVVAAALAQVAPALPRP
                     GAADATELPVLDVPCQPVMQLESLRCWSWRKHRSYPADFLGQDYDVAIPAFHYGDVRF
                     EPGSTHEIAALPDGRTVRIARDKTAEAAAWRAFQNAGFQPVKPGWLMIAGSTPAGLHG
                     LESEAHWTHFFSLLAPALRDAGWRIDCPADFRHRVLTPGEWFADISETDDGGFAVALG
                     VEVDGHRRDLAPMLHAVFRQDRRWLDRAQVDAIPDDAPVTILTEDGDRIVLPAGRLKP
                     IARTLVDLFDSPAGALKVSRLDAPRLAEAFADGWRAEGLASVDAWIERLRGAGGIAPV
                     PPPAGFAHDLRPYQREGLAWLQHLRQHRLGGILADDMGLGKTAQTLAHLLTEKHAGRL
                     DKPALVVLPTSLVFNWQQEAARFAPALRVLGLRGSSRADAFARIPEHDICLTTYPLLW
                     RDRDALSAHAYHSLILDEAQTVKNAASQAAQVVRGLRADHRLCLTGTPLENHLGELWS
                     QFDFLLPGFLGDAKDFTTRWRTPIEKHGDLIRRDILARRIAPFILRRRKEDVAKELPP
                     KTIVVRSVELDGRQRDLYETVRASMDQRVRDEIAARGFARSQIVILDALLKLRQVCCD
                     PRLLKTDAAAKVKERAKLDLLMDMLPELVEEGRRILLFSQFTAMLQLIAAELTARKIP
                     FVTLTGDTDDRAAPVRAFQEGSVPLFLISLKAGGVGLNLTAADTVIHYDPWWNPAVEN
                     QATDRAHRLGQTKAVFVYKLVVAGSIEEKILALQEKKAELAAGILAEDHEGSVKFGED
                     DLRALLAPLPETPREEADAAPKKRGRPKKTPG"
BASE COUNT         3233 a         6278 c         5886 g         3070 t
ORIGIN      
        1 ctggccggtc tgatcgacgc cgcgttcctg atcggtgtcg gtatcgccat gatgtttgcg
       61 ttcgccaacc cgttcaagat ctaagttcgc ggttcaacct gactaaagga agactaccgt
      121 gaatctgaac gcaaccctgg tagcccagct cgttgtgttc ttcattctgg cgtggttcac
      181 gatgaaattc gtgtggcccc ccatcgtgaa ggcactcgac gagcgcgcga agaagatcgc
      241 cgacgggctg gcggctgcgg acaaggcgaa gagcgatctc gcacatgccg agaagaaggt
      301 cgtggaagag ctgcgcaagg cccgcgagtc cgctacggac gtgcgtgccg ctgccgaaaa
      361 gcaagccgcc tccttcctcg acgaagcgcg cgccgaagct gcccgcatca tcgcccaggc
      421 ccgtgaggca gccgaagccg aagccggcgt cgccgcccag cgtgccaagg aagccctgcg
      481 cgatcaggtc gcccatcttg ccgtcgccgg cgccgagaag atcctccgca aggagatcaa
      541 cgctcaggcc cacgccgaac tgctggccaa cctgaaacag gaacttcaat aagccatggc
      601 cgagaacgtc accatcgcac gcccctatgc tgaagcggct tttcagctcg cttccgcggg
      661 caatgcgctg gggccctggt cggaagcact tgatcggctc gcgggtatcg cggccgatcc
      721 gcaaatgcgg gattgcatca gtgatcccaa gctgcagcct caacaactgg ctgggctgtt
      781 tctcgacgtc gccggaagcg gactgactgc tgaacagcag aactatgtcc gtgtcctggt
      841 cgataacgag cgtctgcagg tgcttcccga gatccgtgac ctgttcgttg ctctcaagaa
      901 cgaacacgaa ggtgtcaagg aggcgaacat cgtctccgct ttcccgatgg acgaagccac
      961 gttgaaggcc cttgtggccg accttgaagc ccgcttcaag gcccgtctca acgtgagcgt
     1021 cagcgttgac cccgaactga tcggtggggt caagatcgct gtcggggatg aagtcatcga
     1081 cgcctcggtc cgcggcaagc tcgcgaacat ggccgccgcg ctaaagaact aggagcataa
     1141 gctatgcaac tcaacccctc tgaaatcagt gacctgatca agagtcggat tcagaacctg
     1201 cagctgtccg cgaatgcccg caccgagggt accgtggtat cagtgaccga cggtatctgc
     1261 cgtatccacg gcctctccga tgccatgcag ggcgaaatgc tggaattccc cggcaacgtc
     1321 ttcggtctcg ccatgaacct cgaacgcgac tccgtcggcg ccgtggtcct gggtgaatac
     1381 gaaggcatct ccgaaggtga caccgtcaag tgtaccggcc gtatcctgga agtgcccgtc
     1441 ggtcccgagc tgatcggccg cgtggtcaac tccctgggtc aaccgattga cggcaagggc
     1501 ccgatcaacg ccaagctgtc cgagccgatc gaaaaggtcg ccccgggcgt gatctggcgg
     1561 aagtccgtgt cccagccggt gcagaccggt ctgaagtcgg tggactccat ggtgcccgtc
     1621 ggccgtggcc agcgcgagct gatcatcggc gaccgccaga ccggcaagac cgccgttgcg
     1681 gttgatgcga tcatcaacca aaagggcgaa aacatgatct gcatctacgt ggcggtgggc
     1741 cagaaggcct ccaccatcgc caacgtggtg cgcaagctgg aagaaaacgg cgcgatggaa
     1801 tacaccatcg tcgtcgctgc caccgcctcc gagtccgctg cgatgcagta catcgcgccg
     1861 tactccggct gcaccatggg cgaatacttc cgcgaccgtg gccaagatgc cctgatcatc
     1921 tacgacgatc tgaccaagca agcctgggcc taccgtcagg tgtccctgct gctgcgccgt
     1981 ccgccgggcc gtgaagccta tccgggcgac gtgttctacc tccactcccg tctgctcgag
     2041 cgcgctgccc gcgtgtctgc tgagtacgtc gagaagtaca ccaatggtga agtcaagggt
     2101 aagaccggtt ctctgaccgc tctgcccgtg atcgagaccc aggccggcga cgtgtccgcg
     2161 ttcgttccga ccaacgtgat ctcgattacc gacggccaga tcttcctgga aaccgatctc
     2221 ttcaacgccg gtatccgtcc cgccatcaac gccggtatct cggtgtcccg tgtgggcggc
     2281 gctgcccaga ccaaggtcat caagaagctc ggcggcggcg tgcgtctggc cctggcccag
     2341 tatcgtgagc tggctgcgtt cgcgcagttc gcctccgatc tggacgaagc cacccgcaag
     2401 cagctcgagc gcggtcgtct cgtgacggaa ctgatgaagc agccgcagta ctcgccgctc
     2461 aaggtttccg aaatggcgat cacgctgttc gcggtcgaca agggtgcttt cgacgatgtc
     2521 gaagtcaagc gcgccctcgc ctgcgaagcc gcgatgcacc agtacgtcaa gaccaaggcg
     2581 cccgatctga tcaacaagat cgagacgacc aaggatctcg atgccgacgc cagcaagcag
     2641 ctggccgatg tcatcgcaga gttcaagaag agctgggcgt aacgcgggca tacggagagc
     2701 gaaatggcta gcggcaaaga gatccgtaac aagatcaaga gtgtccagaa cactaaaaag
     2761 atcaccaagg ccatggagat ggtcgctgcg tccaagatgc gtaaggctca ggaccgcatg
     2821 cgtgctgccc gtccctacgc cgagaagatc cgccgtctcg ccgcgaatct gtctgctgcc
     2881 aacatcaccg actacaagca tccgttcctg accaagaagg acgatgccaa gcgtgtgggg
     2941 ctgatcgtgg tcacgaccga caagggtctg tgcggcggcc tcaacaccaa caccttgcgt
     3001 gtggcgctga gcgcactcaa gtcttgggaa ggcaatggtg tcaccgacat ccgtgtgacc
     3061 tgcatcggca acaaggggct gggtttcatg cagcgcctgg gagccaaggt catctcgaat
     3121 gtcacccagc tcggggacac cccccacctg gaaaagctga tcgggcccgt caaggtcatg
     3181 atcgatgcgt tccagaatgg tgagctggac gaagtgcaca tcgcctactc ccgcttcatc
     3241 aacacgatga agcaggagcc ccagcttgag cagcttctcc cgctgaccgg cgagcgtctc
     3301 ggaaccccgg aagggacctg ggactacctc tacgagccgg atgcgcaggt ggtgatcgac
     3361 gaactcctgg tgcgctacgt ggaagcgctg gtgtaccagg gtgtcgccga gaacatggcg
     3421 tccgaacaga gtgcccggat ggtggcgatg aaggcggcat ccgacaacgc agggaacgtg
     3481 atctcggaac tgcaactggt ctacaacaag acccgtcagg ccgcgatcac gaaggaactg
     3541 tccgaaattg tcggcggcgc agccgcggtg taacggttta acaagtttgg ggaatcgacg
     3601 atgagtcaag gaacgatcgt tcaatgcatc ggcgccgtgg tggatgtgca gtttccgcgc
     3661 gacgcgatgc ccaaggttta cgacgccctg accctggaag gtgctgagct ggttctcgaa
     3721 gttcagcaac agctggggga tggcgttgtg cgtaccattg cgctggggtc ttcggacggc
     3781 ctgcgtcgtg gcatggtagt ttcgaacacc ggcaagggta ttgcggtgcc ggtcggtcac
     3841 ggcacgctgg gccggatcat ggatgtgctg ggtcgtccga tcgacgaagc cggcccgatc
     3901 gaatgcgatg agcgccgcgt gattcaccag aaggcgccga agttcgacga gctgtccccg
     3961 tctgtggatc tgctcgaaac cggcatcaag gtgatcgacc tgatctgccc gttcgcaaag
     4021 ggcggcaagg tgggcctgtt cggtggcgcc ggcgtgggca agaccgtgaa catgatggag
     4081 ctcatcaaca acatcgcgaa gcagcacagc ggtttgtcgg tgttcgccgg cgtgggtgag
     4141 cggacccgtg aagggaacga cttctaccac gaaatgaagg actccaacgt tctcgacaag
     4201 gttgcgatgg tgttcggtca gatgaacgaa cccccgggca accgtctgcg cgtggcgctg
     4261 accggcctga ccatggccga gcgcttccgt gacgaaggtc gggacatcct gttcttcgtg
     4321 gataacatct accgctacac cctggccggt accgaagtgt ccgcgctgct gggccggatg
     4381 ccgtccgcgg tgggttacca gccgacgctg gccgaagaaa tgggccgtct gcaggagcgt
     4441 atcacctcca ccaaggtggg ctcgatcacc tccatccagg ccgtgtatgt gcccgcggat
     4501 gacttgaccg acccgtctcc cgcgaccacc ttcctccact tggactccac cgtcgtgctg
     4561 tcccgtgaca ttgccgctct gggtatctac ccggccgtcg atccgctgga ctccacttcc
     4621 cgtcagctgg atccgctggt cgtgggcgaa gagcactaca acaccgcccg tggcgtgcag
     4681 cagaccctgc aacgctacaa ggaactgcgc gacatcatcg cgattctggg gatggacgaa
     4741 ctggcgcccg aagacaagct cctcgtggcc cgtgctcgta agatccagcg tttcctgtcc
     4801 cagccgttct tcgtggcgga agtgtttacc ggttcccccg gcaagtacgt ccccctgaag
     4861 gaaacgatca aggggttcaa gaccatcctg agcggtgaag tggatcacct gcccgagcag
     4921 gccttctaca tggtcggtcc gatcgaagaa gtcttcgaaa aagcgaagac cctctaatca
     4981 tcgcgaccat cgcccggcgg tgctcccagc gggcccgccg gacagaggag gatacaagct
     5041 atggcaatga ccattcacgt cgatatcgtc agcgcggaag agcaaatctt ttccggtctg
     5101 gcggaattcg tggtgctgcc cggcgaggcc ggtgagctgg gcatcatgcc cggccacatg
     5161 ccgctgatga cccgcatcaa gccgggcgca gtgcgagtga aggtccagaa tcaggccgac
     5221 gaagagctgg tgttcgtggc cggtggcctg cttgaagtcc agcccaacct ggtgacggtt
     5281 ctcgcggata ccgcgatccg tggcaaggat ctggacgaag ccaaggccct ggaagcgaag
     5341 cgccgtgcgg aagaagccat gcagaaccag gactctgccc tggaatatgc caaggcccaa
     5401 tccgaactgg catctgcgct ggctcagatc gcggcaatcc agaagctgcg caagcgttct
     5461 cactgaacgc ttgagcggtt tcgacagaag gggcagtccg aaagggctgc cctttttgtt
     5521 tgtgtgccgc ccaagaagtc tgacgacgcg ctgttccgct gttctggcca tttgtctgca
     5581 tcaagcgggt ggatgcccga tcaccggcgg catcgacttt cactgcgctg gcaggtggga
     5641 gcgcaggtca gcggactcga acggccatgt agtgcccctt gcataatccg caagtcgatc
     5701 gcagcgcacc tgcgctgggc gacgggtggt gacgcaacgg agcgctcagc gacgtgcagc
     5761 ttcgaggcgg ctcaggcgtt tgtccagacg ggccagatcg tcccgcagtg cggccatttc
     5821 ggtggcaaag ggatcgaact gcgggcgcgc gaggatgagg ggccgttcct cggtgaagta
     5881 ttcgacgaaa ttctcggtga gattgcgcgc cgcatccaga tgccagcggg tggcatcccg
     5941 cacaccattg atcgcacgat gggctgcaat gtcgcctacc cgttttgcca gggcttcttc
     6001 gccatcccag cgcagatgac gcaggacgaa gccgagcgcg tcggcaaagt ccgcattgcc
     6061 ggtaatgcgg atgtcgccca tcagcgcgtc catgcctccc acggccttgg gcagggatga
     6121 aaccggcagt tcgaggcgaa cgtcgggggt gtcggcggtg gcggaatcgc tgaagaatcc
     6181 gtccccttcg actgctacgc acagtgacgc aggcgcgacc tgaatgacgg cggtgcggcc
     6241 ggcgaagggt ttgaggcgat ccctggccca gggggcgtcc gtgagcagat ggttgagggc
     6301 ggcgatgacg gtggatgtga gcatcggcaa ctccaggcaa gaaaaacggg ggccgcagcc
     6361 cccgccattg taccggtcgt cgtggcgttc agtggcgctg ctggataccg gccaccatcc
     6421 agccgcggga accgtccacg ggtttgacga gatgccagac ttcgtcgaat gccgtcggcg
     6481 cagcgtcgtc ttcctcgcgc agcatgccgt agaagcgggt gctgacgacg tggcgcgaac
     6541 cttcggtggc cacgtccagc accgcggcgt tgagattgac gatgtcggtg cgctgcggct
     6601 tgccatggcg ttcgtcgtac tgcaggcgga tctcggcaaa catctcgggc gacgtgaact
     6661 cgcggaggtc gtccagattg ccggcatcgt tggcggcctg caggcggatg aagttgagct
     6721 tggcctcacg cacgaaggca tcggcatcga agccagaggg aagcgcctcg gtgtcggcca
     6781 cgggggcgcc ggacgtcggc agggcaatgg gtgccgaggc tgcgtgacgg gtttctggtg
     6841 caggggtgga aggcataccc gcatactggg gcgtgggctg ggatgcgcca cggcggaaaa
     6901 gtagcctgaa cagcatcatg acggccatca cgcccaggag gatcagcatg aaggacgctg
     6961 cctgttcgcc caatccgaaa tgggacaaca gtgcggccag gccgatcccc gcggcaagac
     7021 cggccagcgg tcccagccag gaacgtttct ggggcatttg cggtgccgga gcgccggcct
     7081 gcggggcact ctgtgcaggc gctgcagccg tccgcggagg cacctggggt gcaggcgcgg
     7141 cttcgcgacg aacaatggtg tcattacgct gcatgccgaa ggattttccg ccacccaggc
     7201 gggcggcttc ggcgtcgggg acgaccgtgg tcagggtcgc cacggccatc agggcggcaa
     7261 cggccagctt tttcatgggc agttctctcc gggggtgaag gacttacagc ttgtagccgc
     7321 ggtgcagggc aaccacaccg gcggtcatgt tgaagtattc gacccggccg agcccggctt
     7381 gttccatcag atccttgagg gactcctggt ccggatgcat gcggatgctt tccgccaggt
     7441 agcggtagct ttcgccgtcc ttggcgactt tctcgcccat caggggcagc agcttgaagg
     7501 aatagaaatc gtaggccggc gccaggggct tccacacctt ggagaactcc agcaccagca
     7561 ggcgcccacc cggcctgagg acgcgccgca tctcggcgat ggcctggtcc ttgtgggtca
     7621 tgttgcgcag gccaaaggcc acggtgacgc agtcgaagta atcgtccggg aaaggcagct
     7681 tctctgcgtc gcactgggcg acgggcagca ggaagccgtt atccacgacc cggtcgcggc
     7741 cgcggctgag catggcgtgg ttgatgtcgg tgagccacac ctggccggtc ggtccgacct
     7801 ttttggcgaa ggcgagggac aggtctgcgg ttccgccggc cacatcgagc acccggtcac
     7861 cggcgcgcag accggcgatc tgcacggtga aggccttcca caggcggtgc aggccgaagg
     7921 acatcaggtc gttcatcaca tcgtagctgc tggcgaccga cgagaacacg ccggcgacct
     7981 tcttggcctt ttcggcttcg gccactttct ggaagccgaa atgggtggtt ttatcgttca
     8041 tggttcggac aggataaatc ggcccggaag cgccgggcgc gccggggcag atgccgccgg
     8101 gatcagtgct tgtggccgca gccgccggcc ttgggcgcgg caggcgggag ctcggccgga
     8161 tcgcggctgc ccccctccac cgcgaggcgg gtgaggtagg tctgccacag ttcgtcgcga
     8221 cgttcgccga tctcgtaaag gtagtcccat gaatacagcc cgctgtcatg accgtccgaa
     8281 aagatcggac ggatggcgta attgcctacg ggttccacat tcacgatctc cacgtcgcgc
     8341 ttgcccagtt gcagcacttc ctcgcccgga ccgtggccgc gaacctcggc ggagggcgaa
     8401 tggacacgca ggaattcgta gctcaattcg aatcgttgcc cgtcgtcgaa agtgatttcc
     8461 aggattcggg aacgctggtg gagcttgatc tcggtgggca gcggatcgcc cttctggagg
     8521 ccggccatgg gaagcttcct gtgcaatgac gtactgtgcg catgataccg gaacggcccg
     8581 gaccgccgcg tgtcggcacg gggtggtcgt tgcgtttcag cagcgtgacc tgtcacgaaa
     8641 agttcaagat gctgaaatac actctggaca acgcaattcc cgggaggctc ccatgcccgc
     8701 cacgatcctg cttgtcgaag acgaacccgc gatccaggaa ctgatcgctg ccaacctgct
     8761 gcgcgccggt caccaggtgg tgcgggcggg cgatgccgaa accgcccagc gcatcgtgcg
     8821 cgatgccttg cccgatctgg tgctgctgga ctggatgctg cccggcatgt ccggcatcga
     8881 tttcgcccgc cgcctgcgcg ccgacgagcg cacccgcggg attccgctga tcatgctgac
     8941 ggcccgcggc gaggagcagg acaaggtgct gggcctggag gtgggcgccg acgactacgt
     9001 caccaagccg ttctcgcccc gtgaactgat ggcccgcatc aaggccgtgc tgcgccgtcg
     9061 cgcaccggaa tccaccgaag acccggtgga cctcggcggc ctgcgcctgg atccggccac
     9121 ccatcgtctg actgccggtt ccacgccggt cagcctgggg cccacggagt tccgtctgct
     9181 gcatttcctg atgacccacc cggagcgggt ccattcccgt gcccagctgc tcgaccaggt
     9241 gtggggcgac cacgttttcg tggaagagcg gaccgtcgat gtgcacattc gccgcctgcg
     9301 ctgcgcgctg gagccgaccc atcacgacgg attggtgcag accgtgcggg gcagcggcta
     9361 tcgcttctcc acgcagacgg aaagccagcc ggcagtgcga taatcagcat ccgggctgac
     9421 tggcccggat cgtctcacaa aggaatccgt cattcgtccg atccgctacg tggtggtcgg
     9481 ggtgctcgtc gtcctggccg tcatctttgt cctggggctc ggcgtgggcc tcttcttcgg
     9541 gccggtgccc ggtctcacgg tcttcagcgt cgccttgctg gcggccggcg tgtaccacat
     9601 cggcaacctc ttcaagctcg tcaagtgggc gcgccagccg gtcggcacgc ccctgccgcg
     9661 ggctgtcggc ctgtgggact tcgtttacgc cgacctcaac cgcctgatcc gccaggccca
     9721 ggaccagcgc gccaagctca cggcggcgct ggcccgcttt cgcgaagcca gtcaggccat
     9781 gccccacggc atgatgttcc tgactgacac caacttcatc gagtttgcca accgtcaggc
     9841 cgagctgcat ttcggcatcg acaatgcccg cgacaagggt gcgccggtca tcaacctggt
     9901 ccgccagccc gatttcgtgc gctacatgaa cgaaggtcgc tttgacgagc ccctcacctt
     9961 ctcccagttc cggccccaga gccgcaacct gatggtgcac gtggtgccct tcggcgaagg
    10021 catgcgcctg ctcatgtccg aggacatcac ccagggtgag cgcctcgaga ccatgcgccg
    10081 ggacttcgtc gccaacgtct cccacgagat gcgcacgccc ctcaccgtgg tgcgcggttt
    10141 cctcgagacc gtgctcgacg gcctcgacga cctggacaag gatgacatcc gccactacct
    10201 cgccatggcc ggcgaacagg ccgggcgcat ggggcgcctc atcgaggatc tgctcgccct
    10261 gtcggccctg gagaccggcg caccgatgcc ccacgaggaa ccggtggaca tcgcccgcat
    10321 gctcaaggat gtggagcagg aggcccgcgc cgtgtcggcc gagcgccaca gcatcgtggt
    10381 ggacccaccg gcgcccggca ccgagcgcat tctgggcaat tccaaggaac tgcacagtgc
    10441 cttcgccaat ctggcggtca atgcggtgcg ctatacgccg gccggcggcc acatccacat
    10501 tgcctggcgc ttgcgcgggg ccgtggtgga gtattcggtg gaagacgacg gcatcggcat
    10561 cgcgccggag cacatcccgc gcctcaccga gcgtttctat cgggtggatc gcggtcgatc
    10621 acgggagacc ggcggtaccg gtctggggct cgccatcgtc aagcacgtgc tgagccgcca
    10681 tcaggcggat ctgcacatcg acagcgagcc gggtcgcggc agccgcttca gcgcctgctt
    10741 cccggcgcgc cggctggcgg ccgccgaata gcgacttcag ccgccgatga cctcataggt
    10801 cgcctggtcg aagttggtac cgcgggcgat caggctggtc agccggatct ggcggatctc
    10861 ccccggcgag atgtccagca cccggttggc ctggaaccag ccgctgggca tcacgatggt
    10921 ggcctgggcc cgcatcgccg ggatcgccgg cagcatgaag gcctgggaat agggctcgtg
    10981 ggccgataca ttgacgctca ccggccgcgc cgccaccacc gtgggcaggc cggacagcag
    11041 atggatgccg gccatcaggg tgccgtccgc ctcgaacatc agccagctca cctggcccag
    11101 cagatagggg ccgccgtcct cgggcttgat ccccaccagc tgatggtgct cgatgcgctg
    11161 ggcaggctgg cggcgcatca ggcggaatcc ggacaccgaa tgatccaccg tctgccactc
    11221 gtcgatggcg tggccgcggc gctggacctc ggcttccgga tcgcggatgt cctcgatgtc
    11281 atcgtcggcg cgggcgccaa aggtgagcat gttcatgtcg tagcggctgc ccatcatctg
    11341 gcgccggttg gggggcacga actccttgcc cgacaccagc atgccgatgg cgtcccattc
    11401 gccgcagatg cgattcacgc cgcgcgtcgg gcgccgcgga aagcgccgtc cggccgccga
    11461 catgccccac gggcgataga gcgacaccag cagacgggcg caggcgggct gcgcgcagtc
    11521 gtcgcccaga ccgagggagg cgggcgtggt gccctgcttg aactcggcga gcatggcctt
    11581 gatctgggcg gcgaggcggt ggccgtcgaa gcgccgcatg cccggccggt tgccgatcag
    11641 cgcgatgggg cgcaggccat ggtcttcgct gggatcgagg ccgtaggtgc gtgtgtcgtc
    11701 cttgtcccag gtggtctgca tgatgcagta gggcgcaaag cgctgggccc agcggcagat
    11761 ccaggtcagc tcgcgctggt tgcggccgta ggggttggtc aggtcgatca gcagcagggc
    11821 gatgtaggcc tcgttggggc tctgcgcctt ccaggtgttg ttgagcgggt caatgacgcg
    11881 gctgtgggtg atgccggccc gctcggcttc gaggaaggcc gcatgcacgg cgcgccaggc
    11941 gcccggcggt tcggcccggt gggcacgata gtgctcgacg atgatctggc ccaggtactg
    12001 gatccggcgc tgggcgagga gcggcagctg ggcgcggaat tccgcatcgt tggcgccgct
    12061 gtcgcccagg cgggcgtagg ccgtgctgag ggccgtccac aggcgcacca cgtggcgcag
    12121 ggtcgcatcc tcggcgctgt ccggcggcag gggatgggct gcgtaacggg acgacatctc
    12181 gtgctggacg aaggtgatcg tctgtcgggc ggattcgagc acctccagct gctcctcggg
    12241 ccccagcggg ctggcgagca ggccttcgag catgcgcacc agggtggcgt ggctctcgga
    12301 ggcgttgagc gtgtggagat tgcgcagcag cttgcggtat tgctcgacgc tgcgcagttc
    12361 ggtgaaggtc acggcgttgt ccgacatgga tggatcctcc gggctcagcc ggaaaggcgc
    12421 tcggcaatgg cgttgcgcag ttcggtgaag gtcacggcgt tgtccgacat ggatggatcc
    12481 tccgggctca gccggaaagg cgctcggcaa tggcgttgcg cagggcgccg gccagatgcg
    12541 cgtcgtcgac ggccggggca ggcgagaccc cgccccggcg ctgggcttga ccccagaccg
    12601 ggtgggggaa gtggcggtcc tcggtatagc gcgggatcag atgccagtgc aggtgcggca
    12661 ccacgttgcc aaaactggcc aggttgatct tgtccggccg gtagcactcg cgcagcgcca
    12721 cctcgacggc catcaggatg ttgaagcagt ggcgctgatc cgccgtcgac aggtcgctca
    12781 tctcggccac gtgggtgttc cagatcaggc ggcagtagcc cggataatcc gcatcgtcca
    12841 cccggatgac ccgacagctg gcgtcttgcc agagtaccgt ttcccgttcg ggaaagcaca
    12901 gggggcagtc tttcggtgcg ctgtggggca tggcgtgatt gactcgtttt tgcaagattc
    12961 agagatttta atcggcccac atcataagag ggcggacacg gtttgcattg gcacgattcg
    13021 tataaatagt gatacggctc atgccacgac ggattcggtc cggatgaagg cgcgggtcga
    13081 ggcctcaccg gcagcttccg gcgcgccgtc tacaaacagc atcaggctgc ccggcgcgat
    13141 cggggtccac acctcgtcat cggtgagggg cgtggtggcc accaccgcca cccggtcgcc
    13201 gggggtcgtc aattcactga agtcgacggt gatgtcctgg tccttcaggt gcgcctgcgc
    13261 aaagggcgcc tgacgcacga tgtagctcag gcgcgacgcg cagtgggcga acaggcaggt
    13321 gccgttggag agcaggaagt tgaactcccc gtgggcgctg acggccgtgg ccagctcgcg
    13381 gatcgccgcg aacagcgccg gacgggccgg ctcgccgtcg gggaagcgcg tccgcaggga
    13441 ttccagcatg aagcagaagg cccgctccga atcggtctgg cccaccgggc ggaaatcgcc
    13501 gcgcaggggc ggatcgaact cgcccaggtg gccgttgtgg gcgaagaccc agtagcggcc
    13561 ccacagctcg cgctggaacg ggtgcgtgtt ctcgagcttg acgatgccgc gggtggcctt
    13621 gcggatgtgg gcgatgacgt tgagcgaacg gatcggatac tgccgcacca gctcggccac
    13681 cgggctgcgg cagctcgccg ccggatcgag aaagacccgc acgccgcggc cttcgaagaa
    13741 cgcaatgccc catccgtcgc cgtgcacgtc ggtgaggccg ccccgggcct ggaaacctgc
    13801 aaaggaaaag cagatgtccg tgggcgtgtt gcagttcatt ccaagcaatt ggcacatgac
    13861 gctgatttcg gctgtagcgg gcgtttctgt atagtggtcc ggttcaaggc ccgtggcggg
    13921 aatgccgtaa gcactgccgc atctcgccgc cttttcccct cttcaagaag ccatcggcat
    13981 gacattcgat accttaaacg atcactcgtc ggccattgag gccgtgggtc gcctcttatc
    14041 gaccgcacgg cgcgtcctgt tcatcacggg tgccggcatg tctgccgact ccggtctgcc
    14101 cacctatcgg ggcgttggcg gactttacga aggcaccgtc accgacatcg gcctgcccat
    14161 cgaaaaagcc ctctccggcg cggtgttcgc gtcccggccc gacatcacct ggcgctatct
    14221 ggcgcagatc gaatccgcct gtcgcagcgc ccggcccaac gccggccact acgccatcgc
    14281 ccgcctggaa gcccagcgcg gcggcgtcac cgtcctcacc cagaacatag acggcttcca
    14341 ccggggggca ggttcccggg acgtcatcga aattcacggc accctgcacc gcctgcgctg
    14401 cgtgagctgc ggacgtgccc gcgaggtggc cgactatgcc ggcctgtcga tcccgcccga
    14461 atgcccccac tgcggcggcg tgctgcggcc cgacgtggtg ctcttcggcg agaacctgcc
    14521 cgaggccgcc gtgcagcgct tcgaagccgc cctcgccgaa ggccccgacc tggtcttctc
    14581 ggtgggcacc ggcagcgcct tcccctacat cgccgggccc gtggtgtggg ccgcccagca
    14641 gggcatcccc accgtcgaga tcaaccccgg caccacgccg gtcagcgaca tcgtctcgat
    14701 gcgcctgccc ctgcgcgccg gcgaggcgct gcccgcgctc ctcgatgccg ccggactggg
    14761 cctggagggc atccgtccgg actgagtggc ggggccgtgg gctgacgtag aatccgcaat
    14821 tccgcgtcca ccttcttctt gtcatggacc tgcaacgcct tgtcgcccag atcgacgacg
    14881 ccctgctcgc cgagctgttc tccgccaacg ccctggccaa ggcccgcgcc tacgtgggcc
    14941 gcgtgcagca catcgaagcc gccggccacc agatccaggc cctcgtgcag ggctccgacg
    15001 ctgcgcccta ccacgtcacc gtacgcatcg agcggcgcga attcttcggc aagagctcca
    15061 tcgagctcgc cacccgctgc acctgccccg tgggcaaccg ctgcaagcat gccgccgccc
    15121 tggtgctcgc cgcgcgccgc gccggcgccc tggtggacaa accccgcgcc gagatcctca
    15181 actgggcccg cagcctgggc gaacgcctcg ccaaggccga caagaaccgc cgcaaggctg
    15241 ctgccagcga agccatcttc tacctgtgca gcgccgcgca atacggcgac gacgtggagt
    15301 tcggcctgct caagggccgc gtcggggccg acgggcatcc cagcggccgc gccagcgcct
    15361 ggtacaacta cgaccaggcc ctgctcaagc ccccgtcctt cgtccgtgac gaagacctgc
    15421 aggtcttccg cctcctgcgc gaagtcgtcc gccacggcgc cggctacggc cgcccgcgcc
    15481 tgcgcggcac cgaaggcctg gcccttttcg aagccgccct cgccaccggc cgcgcctatc
    15541 tggaactgga cgacaacggc cgcatccagc ccctgcaggc ggccgccccg cggcccggcg
    15601 cgctgatctg ggcacctgac gaagcgggcc tgcgtccgcg ggtggcggtc acccccgccg
    15661 cgcccctggt gatccgcacc gaacccctcg cctggatcga ttcggccacc ggcgaagccg
    15721 gccgcgtgaa catcgacggc ggcggcgccc tcctcgaagt cctcgccctg ccgcccctgt
    15781 cgaccaccga aatccccgtg gtggccgccg ccctggccca ggtcgcgccc gcgctgcccc
    15841 gtcccggcgc cgccgacgcc accgagctgc ccgtcctcga cgtgccctgc cagcccgtga
    15901 tgcagctcga atccctgcgc tgctggagct ggcgcaagca ccgcagctac ccggcggatt
    15961 tcctggggca ggactacgac gtggcgatcc ccgccttcca ctacggcgac gtccgcttcg
    16021 agcccggcag cacccacgag atcgccgccc tgcccgacgg ccgcaccgtg cgcatcgccc
    16081 gcgacaaaac cgccgaagcc gccgcctggc gcgccttcca gaacgccggc ttccagcccg
    16141 tcaagcccgg ctggctgatg atcgccggca gcacgccggc cggtctccat ggcctcgaga
    16201 gcgaagccca ctggacccac ttcttctccc tgctcgcccc cgctttgcgc gacgccggct
    16261 ggcgcatcga ctgccccgcc gacttccgcc accgcgtgct cacccccggc gaatggtttg
    16321 ccgacatcag cgagaccgac gacggcggct tcgccgtcgc cctgggcgtc gaggtggacg
    16381 gccaccgccg cgacctcgcc cccatgctcc acgccgtctt ccggcaagac cgccgctggc
    16441 tcgaccgggc ccaggtggac gccattcccg acgacgcccc cgtcaccatc ctcaccgaag
    16501 acggcgaccg catcgtgctc cccgccggcc gcctcaaacc catcgcccgc accctcgtcg
    16561 acctcttcga cagccccgcc ggcgcgctca aagtctcccg gctcgacgcc ccccgcctcg
    16621 ccgaagcctt tgccgacggc tggcgcgccg aaggcctcgc cagcgtggac gcctggatcg
    16681 aacgcctgcg cggcgccggc ggcatcgccc ccgtcccgcc ccccgccggc ttcgcccacg
    16741 acctgcgccc ctaccagcgc gaaggcctcg cctggctgca acacctgcgc cagcaccgcc
    16801 tcggcggcat cctcgccgac gacatgggcc tcggcaagac cgcccagacc ctcgcccacc
    16861 tcctcaccga aaagcacgcc ggccgcctcg acaaacccgc cctcgtcgtc ctccccacct
    16921 ccctcgtctt caactggcag caggaagccg cccgcttcgc ccccgcgctg cgcgtcctcg
    16981 gcctgcgcgg cagcagccgc gccgacgcct tcgcgcggat tcccgagcac gacatctgcc
    17041 tcaccaccta cccgctgctg tggcgcgacc gcgacgccct ttctgcccac gcctaccact
    17101 ccctcatcct cgacgaagcc cagaccgtca agaacgccgc cagccaggcc gcccaggtcg
    17161 tgcgcggatt gcgcgccgac caccgcctgt gcctcaccgg caccccgctg gagaaccacc
    17221 tgggcgaact gtggagccag ttcgacttcc tcctccccgg cttcctcggc gacgccaaag
    17281 acttcaccac ccgctggcgc acccccatcg aaaagcacgg cgacctcatc cgccgcgaca
    17341 tcctcgcccg ccgcatcgcc cccttcatcc tgcgccggcg caaggaagac gtcgccaaag
    17401 agctcccccc caagaccatc gtcgtgcgca gcgtcgaact cgacggccgc cagcgcgacc
    17461 tctacgaaac cgtgcgcgcc agcatggacc agcgcgtccg cgacgagatc gccgcccgcg
    17521 gcttcgcccg cagccagatc gtcatcctcg acgccctcct caagctgcgc caggtgtgct
    17581 gcgacccccg cctcctcaag accgacgccg ccgccaaggt caaggaacgc gccaagctcg
    17641 acctcctcat ggacatgctc cccgagctcg tcgaagaagg ccgccgcatc ctcctcttct
    17701 cccaattcac cgccatgctc cagctcatcg ctgcggagct caccgcgcgc aagattccct
    17761 tcgtcaccct caccggcgac accgacgacc gcgccgcccc cgtgcgcgcc tttcaggaag
    17821 gcagcgtccc cctcttcctc atcagcctca aagccggcgg tgtcggcctc aacctcaccg
    17881 ccgccgacac ggtgatccac tacgacccct ggtggaaccc cgccgtcgag aaccaggcca
    17941 ccgaccgcgc ccaccgcctc ggccagacca aggccgtatt cgtctacaag ctcgtcgtag
    18001 ccggctccat cgaagaaaaa atcctcgccc tgcaagagaa aaaggccgaa ctcgccgccg
    18061 gcatcctcgc cgaggaccac gaaggcagcg tcaaattcgg cgaagacgac ctccgcgccc
    18121 tcctcgcccc cctgcccgaa accccccgcg aagaagccga cgccgccccc aaaaaacgcg
    18181 gccggccaaa gaagacaccg ggctgaggaa acagaccgcg ccgcggcggc cacaccctcc
    18241 gccgcgccca tttccaagat ccgcccccag aatcccccgc gcccgtcaca aaagaccctg
    18301 cggacccgtg cacagaccct gtgcaccgct gctcagatcg tttccatcgg tgctcagacc
    18361 atgtcgaccg gtgcacaccg tctgtcgacc cctgcgcaga cgatgtcgac ccgtcaacag
    18421 ccttgtcgcc gcccccgaca cacaggattg atagcccttt ccatcat
//
LOCUS       DAOQMM010000072        18307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1137_length_18307_cov_57.523504, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000072 DAOQMM010000000
VERSION     DAOQMM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18307
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_18307_cov_57.523504"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /gene="atpE"
                     /locus_tag="PLH85_11850"
     CDS             complement(<1..217)
                     /gene="atpE"
                     /locus_tag="PLH85_11850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNB65303.1"
                     /translation="MEHVLGFVALAAGLIIGLGAIGACIGIGIMGSKYLEASARQPEL
                     MNALQTKMFLLAGLIDAAFLIGVGIAMM"
     gene            complement(280..1122)
                     /gene="atpB"
                     /locus_tag="PLH85_11855"
     CDS             complement(280..1122)
                     /gene="atpB"
                     /locus_tag="PLH85_11855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNB65304.1"
                     /translation="MSTGHEAHAPTAGEYIVHHLTHFNSTGHAQSAVIDWSVWNLDTL
                     FFSVGLGIVTLLILYKAASKATSGVPGRFQAAIEILVEMVADQAKGIVHSAESRKFVA
                     PVALTVFFWIFLMNSMDFLPVDLLPKIWAIMCGDEHAFLRVVPTADLNGTLGMSIGVL
                     LICLYYNIKIKGFGGWVHELFTAPFGSHPLLYPINFLMQMIEFLAKTVSHGMRLFGNM
                     YAGELVFMLIALMGGTATVFGFLGHVVAGSIWAIFHILIVALQAFIFMMLTLVYIGQA
                     HEGH"
     gene            complement(1125..1466)
                     /locus_tag="PLH85_11860"
     CDS             complement(1125..1466)
                     /locus_tag="PLH85_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNB65305.1"
                     /translation="MFKAVTLQIGATILAALIAGAFLGLRGALSAALGGAACFLPNLL
                     FALRLHLVTKRPDASYPVAFFVGEFFKIASTVGLLATVRLVYPDVHWLALFGGLVVAL
                     KANLFAFLVKT"
     gene            1652..1963
                     /locus_tag="PLH85_11865"
     CDS             1652..1963
                     /locus_tag="PLH85_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB65306.1"
                     /translation="MNKDYSRAIPAAWRDVSAVFAALGDEHRQRILLTFEAGERLNVG
                     QIAEVSTLARSTVSHHLKVLREAGVLSAQRQGKEIYFSVNTHFLQKAFARVSDYLETR
                     L"
     gene            2338..3171
                     /gene="rhlP"
                     /locus_tag="PLH85_11870"
     CDS             2338..3171
                     /gene="rhlP"
                     /locus_tag="PLH85_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922374.1"
                     /note="RhlP (RHombo-target LipoProtein) is a family of
                     predicted lipoproteins that, in general, co-occurs with a
                     form of rhombosortase, and that has an apparent cleavage
                     site for that enzyme, a GlyGly motif, near the C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget lipoprotein"
                     /protein_id="HNB65307.1"
                     /translation="MRPFPSALVLSLAASVSIAGCATWFNPAPHTQRATVVEYLYPKG
                     EQPTLTPSIPELHLPLRVGIAFVPSERFDDLPETERDRLLNRVRDAFANRPYIAAIEI
                     IPTAYLRPRGGFDNLEQAARMFNVDVVTLLSYDQVQFSDSNRLSVFYWTLIGGYIFNG
                     SQYDVSTLVDASVFDVKSRSLLFRAPGTSQITGSSPLVNFAEASREARSEAYRRAIDV
                     LIPRLDAQLEAFKVRVKDRQVARIVEKPGYSGGGALDPVALGLLGTGALLALAWRRRS
                     A"
     gene            3221..3748
                     /gene="rrtA"
                     /locus_tag="PLH85_11875"
     CDS             3221..3748
                     /gene="rrtA"
                     /locus_tag="PLH85_11875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HNB65308.1"
                     /translation="MLVTLLPADAVAWRRDALGDGAWWRLWTGPFSHWSALHLAGNLT
                     AAAALSVLIGPPVWRWIAVLPAVAPLLSLFLIASAPDLQQYRGLSGLLGVLVVGAGVE
                     GGVIGRLVAGLYVVKLAVDAIEGASSPVLPTGVAVVWQAHLGGLLLGLLVAASFHLHR
                     RASASTPTRTPSDTP"
     gene            3745..5625
                     /locus_tag="PLH85_11880"
     CDS             3745..5625
                     /locus_tag="PLH85_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB65309.1"
                     /translation="MSTQFHLLRERRFLPFFVTQFLGAFNDNLFKNALVVVLTFQATR
                     WTTIRPELLANLAAGLFILPYFLFSASAGQLADKYDKARLTRLVKLLEMLIMGVALAG
                     FLLHTLMVLFVALFLLGCHSTLFGPVKYALLPQHLREDELVGGNALVEAGTFVAILLG
                     TLAGGLLAGMDSAERWIAVAGFLVAATGYLSSRAIPAAPPPAPTLRIHPNPFTETWRN
                     IGFARQNRPVFLAILGISWFWLYGALFLAQFPAFAKNVLGGGEGTVTLLLAVFTVGIG
                     LGSLLCERLSGGHLEIGLVPFGSIGLTLFGLDLALAAPAGVPAAAPLALSGVLAQPGT
                     LRLLVALLMLGIFGGFFIVPLYAMMQSRSAVGHRARVIAANNILNALFMVVGALGAAA
                     LLAAGLSIPALFAAAAVMNAAVALFIYRLVPEFLLRFIIWLLVHSVYRLRTEGLPHLP
                     SEGPAVLVCNHVSFVDALVIAAACRRPIRFVMDHRIFRWPVLSFVFRHSRAIPIAPAR
                     EDTAMMEAAFDQVAAALAEGELVAIFPEGQITRDGELNAFRPGIRRILAASPVPVIPM
                     ALRGLWGSMFSRKDGPALSRPLRRGLFNAIELAVGAPLSPESATVEGLRARVLALRGE
                     RR"
     gene            5656..5886
                     /locus_tag="PLH85_11885"
     CDS             5656..5886
                     /locus_tag="PLH85_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65310.1"
                     /translation="MSYLITLIQAFVLLILLCLILAGGVCVASFPTEPAAVVVGLISM
                     LTFSWPAWLLIRTFRSRPAPKTEPERSDPPTP"
     gene            5883..6647
                     /locus_tag="PLH85_11890"
     CDS             5883..6647
                     /locus_tag="PLH85_11890"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB65311.1"
                     /translation="MIADPATAHALHAMFEALALFGGARLYVHQSQRQSRRSVFQGGR
                     FAVLCGCLLGAAIGNKVVFWLEMPQLFQQADGWQRIFAGQSVVGGLLGGLIGTEIAKA
                     LSGETASTGDGFVVPVLAGIAVGRVGCFLAGLHDGTFGRPTALPWGIDFGDGIPRHPT
                     QLYEILFVVTLAGLLQQLSPRLGAQPGLRFKLMLSAYLLWRLLIDGLKPLPYAWPFGL
                     SGIQWVCTLALSIYLPLTIRQWRQRHVTQEPPLPVL"
     gene            6613..8058
                     /locus_tag="PLH85_11895"
     CDS             6613..8058
                     /locus_tag="PLH85_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB65312.1"
                     /translation="MSRKNRPYLFYDTTASVCSTCLHRVEAKILIKDDRVYLEKWCPA
                     HGRERVLIADDADYYRLAREVYVKRPEMPEHFSTTMTWGCPYDCGLCPDHMQHSCLTV
                     LEITEHCNLKCPVCYADSGPERQLHRSLGEIEAMLDAVVRNEGEPDVVQLSGGEPTLH
                     PDFFAILDAARARPIRHLMINTNGLRIARDPEFVARLAEYGRGLEIYLQFDSLRAEAL
                     HTLRGADLTRMHAQALEALEHHGLSTTLVMTLRNGVNDQEIGDVIRHGLGYRCVRGVT
                     LQPVQDAGRNLGGRGAEDRLTVSGIRRRIAEQSGLFTLDDIVPVPCNPDTLAMGYALR
                     LDGQVHPLTRWLGPEALLAGPANTIVFERDPSIRDRLFRLFSTNHSPASGARCLSDLL
                     CCLPRIDAPTLDYTNVFRILIVQFMDATNLDLRALKKSCVHMVQPDGRMIPFEAFNLF
                     YRGRLTEGLETLRASVADDIARRHRSVPSAG"
     gene            complement(8047..8811)
                     /locus_tag="PLH85_11900"
     CDS             complement(8047..8811)
                     /locus_tag="PLH85_11900"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HNB65313.1"
                     /translation="MRARLLMAAALLLCVVPVGAELIHGSAIYRERLRLPPEAVLEVT
                     VEDVSRADAPAPVIGRTRLEPAGAPPIPFAVEVDPADVEAHRRYSLRGRISVGGAPWF
                     VTDQVYPVLDGGPDGAVTLLLRRVSSRPPVAGDSGGLRGAPWRLITQDGFPVDPQRSV
                     RIPQLHFLEAGRLAGTGGCNRFIGTYRVDGDHLRVSAHAGTKMACGQGMDAEVGFHAL
                     LDQVTHWRIEGATLLLLDDAGRLVLSLERTGSALAR"
     gene            complement(8888..9604)
                     /locus_tag="PLH85_11905"
     CDS             complement(8888..9604)
                     /locus_tag="PLH85_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNB65314.1"
                     /translation="MDSRSTFDSPSGFNRRQFLGASLGAVSALALGGFVRPAQAASPI
                     VLPALPYADSALAPVISANTLGFHYGKHHKAYVDNLNKLVAGSELADLSLEQIIAKVM
                     GKADKTAIFNNAAQAWNHTFYWYSLRPNGGGEPPAALKARIEASFGSVDALKKELAQA
                     ALTQFGSGWAWLVADGKALKVVKTGNADMPQAQGYTPLLTLDVWEHAYYLDYQNRRGD
                     YVNAVLDKLINWEFALKNLG"
     gene            complement(9709..10254)
                     /locus_tag="PLH85_11910"
     CDS             complement(9709..10254)
                     /locus_tag="PLH85_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNB65315.1"
                     /translation="MALKSTIFRASLTVSDLDRGHFGEHVLTLARHPSETDERMMVRV
                     LAFALYADERLQFGRGLSSEDEPAAWLKDDTGRILEWIEVGLPDEKLLRRASGRAEQV
                     RVLAYGGRSVDIWWQKDSAALARLGNLTVQALETDDSRALAALAERSMTLTCTLQDGQ
                     IWFSSGDTTLGFSLRTLHAPT"
     gene            complement(10330..10701)
                     /locus_tag="PLH85_11915"
     CDS             complement(10330..10701)
                     /locus_tag="PLH85_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65316.1"
                     /translation="MTTRALALAALIALGLAACSKKEEAPAPTPAPVVDAAKEAAQKA
                     EEAAKQAAEATKDAAKDAAGAAKEAGTAAAGAAKDAAKDAVAAAKEAAGAAKEAGKEA
                     VAATADATKEAADKAKAAVGK"
     gene            complement(10860..11420)
                     /gene="efp"
                     /locus_tag="PLH85_11920"
     CDS             complement(10860..11420)
                     /gene="efp"
                     /locus_tag="PLH85_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNB65317.1"
                     /translation="MKTAQELRSGNVIMVGNDPLVVQKAEYNKSGRNAAVVKMKLKNL
                     LTGAPSENVYKADDKFEVVVLERKEVTYSYFADPMYVFMDADYEQFEVEAENMTDALK
                     YLVDGMPCEVVFYNGKAISVDLPTSVVREVEYTEPAVKGDTSGKVLKPARIAPSGFEL
                     SVPAFVEIGDKIEIDTRTDEYRNRVK"
     gene            complement(11477..12637)
                     /gene="earP"
                     /locus_tag="PLH85_11925"
     CDS             complement(11477..12637)
                     /gene="earP"
                     /locus_tag="PLH85_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNB65318.1"
                     /translation="MNNSTPGSSSDARPCWDIFCPVVDNFGDIGVCWRLARELAVAHG
                     VQPRLWVDDLPSARRLIPSLEPALAIQHRAGVEIRAWTDPLPAIVPGDVVIGAFACSL
                     PAGFLEAMALRLPRPLWINLEYLSAEDWVAGCHRLPSPHPRLPLTQYFYFPGFDASSG
                     GLLRERDYDVRRAAHAPVQCLTGLGLPAARPGELRISLFAYENAALPELLEVWRNGTR
                     PLTCLVPEGRVLPQVAQCLGRNGLKPGDRIEAGALRVVVIPFVEQSRYDELLWACDLN
                     FVRGEDSFVRAQWAERPFVWQIYRQDDDAHLDKLDAFLARYAERLPPSAGAAVRDFWR
                     AWNRGHDAARAWQAFADALPALSAAAPAWSAHLQQAGELAAGLVQFSKDQLK"
     gene            12935..13204
                     /locus_tag="PLH85_11930"
     CDS             12935..13204
                     /locus_tag="PLH85_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65319.1"
                     /translation="MLSITDLLDFIDLDRDTVLIVAEATGLPPEPAIILARELLASRR
                     GILTLHHMFRDERARADERGHVLRVRDLDRAYRHFARKYPQPVVV"
     gene            13535..14446
                     /locus_tag="PLH85_11935"
     CDS             13535..14446
                     /locus_tag="PLH85_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB65320.1"
                     /translation="MSAPSSPISRAVRHPYLLLTLTVLFWSGNMVVGRALREAIPPMS
                     LALGRWILAFLLTLPFAWPHRNRLQTLDRREWGILAVLGVLGVGAYNTLAYIALSHTT
                     ATNAALLNSFIPVAILVLTWWLGHRSRPLEIIGVGVSLAGVMTILSHGELRVLTHLQL
                     NIGDVWMLGAVLSWALYTVCLRWRPEGLHPMALLAVLTAIGLMVLAPLVAWELAGGAQ
                     IRPDAGLLAGIAYTGIFPAFLGYVFYNRAVAQVGAATGGLFIHLMPAFSTLLAMVFLG
                     EQPHLHHFFGIALILAGIGLTTGFKNR"
     gene            14477..14947
                     /locus_tag="PLH85_11940"
     CDS             14477..14947
                     /locus_tag="PLH85_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB65321.1"
                     /translation="MRFEHLVEINDPLNPLIPSLTREQVWSGLMLRVDEPTVFLPGLE
                     RCEILSRAADQVERRLHFGGVQVRDTVTFSPLEWVRFESSANAEHAGGRLTIRLEQPD
                     PLRLYLRFTYETTLPDGGAADGVQVSEFVKSAYRESDVDTVRIIRVLSAGGRPH"
     gene            14996..16123
                     /locus_tag="PLH85_11945"
     CDS             14996..16123
                     /locus_tag="PLH85_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNB65322.1"
                     /translation="MIARPELRSFQPPVRTLMGPGPSDVPPRVLAALARPTVGHLDPA
                     FVGMMDELKELLRYTFQTRNELTFPVSAPGSVGMETCVVNLVSPGDTVVVCINGVFGQ
                     RLAENVRRAGGVPVIVEDPWGEPVDPAKVEDTFKAHPEASLLAFVHAETSTGALSDAA
                     ALAAIARRFGALTLMDCVTSLGGVPVLLDEWGIDAAYSGSQKCLSCTPGLSPVSFSEA
                     AVARIRARKTPCQSWFQDLSLVLGYWSGAKRTYHHTAPVNPLYGLHESLLMLAEEGLE
                     AAWQRHRDNHLRLRAGLEALGLRFVVREDARLPQLNTVWVPDGVDEAAARTRLLKEYG
                     LEIGAGLGVFAGKVWRIGLMGHSSRPANIALCLAALKDVLG"
     gene            complement(16213..16539)
                     /locus_tag="PLH85_11950"
     CDS             complement(16213..16539)
                     /locus_tag="PLH85_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65323.1"
                     /translation="MESDSVLYGLLGRIHLLMRRVTNRITDVEYMRTNKDYAREIVRL
                     ADASGHDELRELAGRLRDAMDLDAAEADTPSTPAPAPRNGFFERLRSPAVSPEGDKDR
                     YVRSLR"
     gene            complement(16563..17807)
                     /locus_tag="PLH85_11955"
     CDS             complement(16563..17807)
                     /locus_tag="PLH85_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNB65324.1"
                     /translation="MAALLALSGPALAQSAPAAHAEGGNTAWMLTATALVLLMSLPGV
                     ALFYGGMVRSKNVLSTMTQTIAIAAVVTLTWMVAGYSLALRPGTPFLGGLDRLFLEGL
                     QAEGLAGGAVPETVYVMFQLTFAIITTALVPGAFAERMKFSAVLLFAALWSVLVYAPV
                     AHWVWEPGGWLAALGALDYAGGTVVHINAGVAGLVCALVLGPRQGFGREPMTPHNLTL
                     TLSGAALLWVGWYGFNAGSALAADSHAGMAMLVTQMAAATAAVTWMAVEWMLRGRPSA
                     LGLVSGAVGGLVAITPAAGYVSPSSALLIGLAAGAGCYIASTSLKRMLGYDDALDAFG
                     IHGVGGIIGALLTGLLASPAIGGSAGRMSAQLAGVGATVAYSALMTLIVLGIVQVITG
                     LRVDATTEADGLDIAEHGEALD"
     gene            complement(18128..>18307)
                     /locus_tag="PLH85_11960"
     CDS             complement(18128..>18307)
                     /locus_tag="PLH85_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HNB65325.1"
                     /translation="GILQVWLQRFSSSPIPFMAAQEQISLFYWLREGTGVVFLVGLLI
                     YVASFFVSGEEKAAA"
BASE COUNT         3092 a         6112 c         5845 g         3258 t
ORIGIN      
        1 acatcatggc gataccgaca ccgatcagga acgcggcgtc gatcagaccg gccagcagga
       61 acatcttggt ctgcagggcg ttcatcagtt cgggctgacg agcagaggct tcgaggtact
      121 tgctacccat gatgccgata ccgatacagg caccgatagc acccagaccg atgatcagac
      181 cggcggccag agcaacaaaa ccaagaacgt gttccatgac gactccttta gttgagatga
      241 tttaaattga aaccgaaacg actgctaaaa ctttgaaact cagtgacctt cgtgggcctg
      301 accgatatac accagggtca gcatcatgaa gatgaaggcc tgcagagcga cgatcaggat
      361 gtggaagatc gcccagatcg aacccgcgac cacgtggccg aggaaaccga acaccgtagc
      421 ggtaccaccc atcagggcga tcagcatgaa caccaactcg cctgcgtaca tgttgccgaa
      481 cagccgcatg ccgtgggata cggtcttggc caggaactcg atcatctgca tcaggaagtt
      541 gatcgggtac aggagcggat gagaaccgaa gggcgcggtg aagagttcgt gcacccagcc
      601 gccaaaaccc ttgatcttga tgttgtagta caggcagatc agcagcacgc cgatagacat
      661 gcccaaggtg ccgttgaggt cggcggtggg caccacgcgc aggaaggcgt gctcgtcgcc
      721 acacatgatc gcccagatct tgggcagcag atccaccggc aggaagtcca tggagttcat
      781 caggaaaatc cagaagaaca ccgttagggc gacgggggcg acgaacttgc gggactcggc
      841 gctatgcacg atgcccttgg cctgatcggc aaccatctcg acgaggattt cgatggcggc
      901 ctgaaagcgc cccggcacgc cggaggtggc tttgctggca gccttgtaga ggatcagcag
      961 ggtgacgatg cccaggccga cggagaagaa cagggtatcc agattccaga cactccagtc
     1021 gatgacggca gactgggcgt ggccggtgga gttgaagtgg gtcaagtggt ggacgatgta
     1081 ttcgcccgcg gtcggtgcgt gtgcttcgtg gccagtgctc atggtcaggt cttcaccaaa
     1141 aatgcaaata aattcgcttt aagcgccacg accaacccgc caaacaacgc caaccaatgg
     1201 acgtctggat acacgagtcg aacggtcgcc aacaatccta cggttgacgc gatcttgaag
     1261 aactcgccaa cgaaaaacgc aacagggtac gacgcgtccg gccgcttggt aaccaagtgc
     1321 aggcgcaaag cgaacagcaa attgggaaga aaacaggctg cgcctcccaa cgccgccgac
     1381 aacgcaccgc gcaatcccaa aaatgccccg gcaatcagtg ctgccaggat cgtcgcgcct
     1441 atctgcaggg tcaccgcttt gaacatggat ctctgcgagg cacgtcgggt gttgcgccgt
     1501 ccgccgtacg aaaaacgtgc ggatggtagg ggtaaaccgc agcaaagtca aatgttggtg
     1561 cagtgcacca tcaacaaaaa ggctgtgttt acgcgggcgt caacggaagt cggccttgac
     1621 aaaccccgca aacgtatcga aagttatcga catgaacaag gactactccc gtgccattcc
     1681 ggcagcctgg cgcgacgttt cggccgtgtt tgccgcgctc ggagatgaac accggcaacg
     1741 catcctgctg accttcgagg ccggcgagcg gcttaacgtg ggccagattg cggaagtctc
     1801 gaccctggcc cgttccaccg tgtcccacca tctcaaggtg ttgcgcgaag ccggtgtcct
     1861 gtctgcccaa cgtcagggca aggagatcta tttcagtgtg aatacgcact ttctgcagaa
     1921 ggcctttgcg cgggtctctg actacctgga gactcgactg tgagcaatgt ccggcgcgtt
     1981 ccatgggatc aggacgtggc cgggcccggg gcacgtcacg gcatcggaac cgcggcgaag
     2041 tgcgcctccg atcggtcgcg gctggcgaca gtctccgtgc caccgtttgt cttccgccta
     2101 cggcccagac cgtcggatcg cgtggatcct gcccaaccca caccgctacg ccgacgggtt
     2161 tccggcaagg atcgatcacc cgcgcacgcg ccatctgcga ccggattcat ccgttcgacg
     2221 gcattcgcac ggcgtcccgt accgtcctac gatctcgatg caccccaaaa cggctcaagc
     2281 ttgggcgcag cccagatgcg cccgacagcc gcctgcgtac cgacactgga gtgatccatg
     2341 cgacccttcc cgtccgccct tgtgctttcc ctcgccgcgt ccgtcagcat tgccggatgc
     2401 gcgacctggt tcaatcccgc accccatact caacgggcca cggtggtcga gtacctctat
     2461 cccaagggcg aacagccgac cctcacgccg tcgatacccg aactacatct gcccttgcgc
     2521 gtcgggatcg cgttcgtgcc cagcgaacgt ttcgacgacc tccccgagac cgagcgggac
     2581 cgcctgttaa accgggtcag ggacgctttt gccaaccgcc cctacatcgc tgccatcgaa
     2641 atcattccca cggcctacct gcgcccccgc ggcggcttcg acaacctgga gcaggcggcg
     2701 cggatgttca acgtggacgt cgtgaccctg ctgtcctatg accaggtcca gttcagcgac
     2761 agcaatcgtc tctccgtctt ttactggacc ctcattggcg gctacatctt caacggcagc
     2821 cagtacgacg tgagcaccct ggtggatgct tcggtcttcg acgtgaagtc ccgcagcctg
     2881 ctgttccgtg ccccgggcac cagtcagatc acgggcagtt cgccgctggt gaactttgcc
     2941 gaagcctccc gcgaggcccg cagcgaagcc taccggcgcg cgatcgatgt gctgatcccc
     3001 cggctcgatg cgcagctgga agccttcaag gtccgcgtca aggacaggca ggtcgcccgc
     3061 atcgtcgaga agcccggata ctccggcggt ggagccctcg accccgtcgc cctcggcctc
     3121 cttggaacgg gcgcactgct ggccctcgcc tggcggcgtc ggtcagcctg agccggtgcg
     3181 ggtcgaacgc ctctgtgccg gcgcgagcgt cgcattcctc atgctcgtca ccctgctccc
     3241 cgccgacgcg gtggcgtggc gtcgcgatgc ccttggcgac ggcgcctggt ggcgcctgtg
     3301 gaccggcccg ttcagccatt ggtccgcgct gcatctggcc ggcaacctga ctgccgccgc
     3361 tgcgttgagc gtcctgatcg gcccgccggt ctggcgctgg atcgccgtcc tgccggcggt
     3421 cgcaccgttg ctgtccctgt tcctgatcgc cagcgcgccg gacctacagc agtatcgcgg
     3481 gctctcgggc ctgctgggcg tgctggtggt aggtgcaggg gtcgaaggcg gggtgatcgg
     3541 ccggctggtg gcgggtctgt acgtcgtcaa gctcgccgtc gacgccatcg agggcgcttc
     3601 ctcaccggtg ctgccgacgg gggtggccgt cgtctggcag gcccatctgg gcgggctgct
     3661 gctcggtctc ctggttgcgg cctcattcca cctgcaccga cgcgccagcg cgtccacgcc
     3721 gacacggacc ccttccgata cgccatgagt acccagttcc atctgctccg cgagcggcgc
     3781 tttctgccct tcttcgtcac ccagttcctg ggcgccttca acgacaacct gttcaagaac
     3841 gccctggtcg tggtgctgac cttccaggcc acgcgatgga cgacgatccg gccggagctc
     3901 ctggccaatc tggcggcagg cctcttcatc ctgccctatt tcctcttctc ggccagcgca
     3961 ggacagctgg ccgacaagta cgacaaggcg cgcctgaccc gcctcgtcaa gctgctggaa
     4021 atgctgatca tgggcgtggc cctggcggga ttcctgctcc acacgctgat ggtgctgttc
     4081 gtcgccctgt tcctgctggg ctgtcattcc accctgttcg ggcccgtcaa gtacgccctg
     4141 ctgccccagc acctgcggga agacgaactg gtgggcggca acgccctcgt cgaagccggc
     4201 actttcgtgg cgatcctgct ggggacgctc gccggcgggc tgctggcggg catggactcc
     4261 gcggagcgct ggatcgccgt ggcgggattt ctggtggctg ccacaggcta cctgagcagc
     4321 cgcgcgatcc cggccgcacc gccgcctgcg ccgacgctgc gcatccatcc caatcccttc
     4381 accgaaacct ggcgcaacat cggcttcgcg cggcagaacc gcccggtatt cctggcgatc
     4441 cttggaattt cgtggttctg gctctacggc gccctgttcc tcgcccagtt cccggccttt
     4501 gccaagaatg tgctgggcgg cggcgagggg accgtcacgc tgctgctggc ggtcttcacg
     4561 gtgggcatcg ggctcggctc gctgctctgc gagcgcctct ccggcggcca cctggagatc
     4621 ggcctggtgc ccttcggctc catcgggctc accctgttcg ggctcgacct ggccttagca
     4681 gcgcccgcag gcgtcccggc cgctgcgcca ctggcgcttt cgggggtgct tgcccagcct
     4741 ggcaccctgc gtctcctggt cgccctgctg atgctgggca tcttcggcgg cttcttcatc
     4801 gtgcccctct acgccatgat gcagagccgc tcggcagtgg gacaccgggc gcgggtgatc
     4861 gccgccaaca acatcctcaa cgccctgttc atggtggtcg gcgcgctggg agccgcggcg
     4921 ctgcttgccg ccgggttgtc gatcccggcc ctgttcgcag ccgccgcggt gatgaacgcc
     4981 gccgtggcgc tcttcatcta ccggctggtg ccggaattcc tgttgcgctt catcatctgg
     5041 ctgctggtcc acagcgtata ccgcttgcgc acggaagggc tgccgcacct cccgtccgaa
     5101 ggtccggcgg tgctggtgtg caaccacgtg agcttcgtcg atgcactggt gatcgccgcc
     5161 gcctgtcgcc ggccgatccg cttcgtgatg gatcaccgca tcttccgctg gccggtcctg
     5221 tccttcgtgt tccgccatag ccgcgcgatc cccatcgcac cggccaggga agataccgcg
     5281 atgatggagg cggcctttga tcaggtggcc gccgcgctgg ccgaaggcga actggtggca
     5341 atctttccgg aggggcagat cacccgcgac ggcgaactca acgccttccg gccgggcatc
     5401 cggcgcatcc tcgccgccag ccctgttccc gtcatcccga tggccctgcg cggactgtgg
     5461 gggagcatgt tctcccgcaa agacggcccg gccctcagcc gaccgctgcg acgcggcctc
     5521 ttcaatgcca tcgaactggc cgtcggtgcc ccactgtccc ccgagtccgc cacggtggag
     5581 ggtctgcgcg cgcgggtcct tgccctgcgc ggcgaacgtc gttgaacggc tcctacttca
     5641 cgtgccccac gcaccatgtc ctacctcatc accctgatcc aggccttcgt cctgctgatc
     5701 ctgctctgcc tgatcctcgc cggaggcgtc tgcgttgcct catttcccac cgaacctgcg
     5761 gcggtggtgg tcgggctgat cagcatgctg acgttcagct ggcccgcatg gttgctgatc
     5821 cgcacgttcc ggtcgcgacc ggcgcccaaa acggaacccg agcggtccga ccctcccaca
     5881 ccgtgatcgc cgatcccgcc accgcgcacg cactgcacgc catgttcgag gccctggcct
     5941 tgttcggcgg cgcccgcctg tacgtccatc agagccagcg gcagtcgcgg cgcagtgtct
     6001 ttcagggagg ccgatttgcc gtactgtgcg gctgcctgct cggcgcagcc atcggcaaca
     6061 aggtggtgtt ctggctcgaa atgccccagc ttttccagca ggccgacggc tggcaacgga
     6121 tcttcgctgg tcagtccgtg gtaggcggct tgctgggcgg gctgatcggc accgaaatcg
     6181 ccaaggcact cagcggcgag acggcgtcca ccggcgacgg cttcgtggtg ccggtcctcg
     6241 ccggcatcgc cgtcgggcgt gtcggctgct ttctggcagg tctgcacgat ggcacgttcg
     6301 ggcgtccgac cgccctgccc tgggggatcg atttcggtga tggcatccct cgccatccca
     6361 cccagctcta cgaaatcctc ttcgtcgtga ccctggccgg cctgctgcag cagctgtcac
     6421 cccggctcgg tgcgcagccc ggtctgcgct tcaagctgat gctaagcgct tacctgctgt
     6481 ggcgcctcct gatcgatggg ctcaagccgc tcccctatgc ctggcccttc ggactctcgg
     6541 gcatccaatg ggtctgcacg cttgccctgt ccatctacct tcccctcacg atccgccaat
     6601 ggcggcagcg ccatgtcacg caagaaccgc ccctacctgt tctatgacac gaccgcttcg
     6661 gtgtgcagca cctgcctgca ccgggtcgag gccaaaatcc tcatcaagga tgaccgggtc
     6721 tacctggaaa agtggtgccc ggcccatggc cgggaacgtg tcctgatcgc ggatgacgcc
     6781 gactactacc gcctcgcccg cgaggtctat gtgaagcggc cggaaatgcc cgagcacttc
     6841 tccacgacca tgacctgggg ctgcccctac gactgcggtc tctgccccga ccacatgcag
     6901 cactcctgcc tcacggtgct cgagatcacg gagcactgca atctgaagtg cccggtgtgt
     6961 tacgccgatt ccgggcccga acgccagctt caccgcagcc tgggcgagat cgaagcgatg
     7021 ctggacgccg tcgtgcgcaa cgagggcgaa ccggacgtgg tgcagctttc tggcggcgaa
     7081 cccacgctgc acccggattt ctttgcgatc cttgacgccg cccgggcgcg gccgatccgt
     7141 cacctgatga tcaacaccaa cggcctgcgc atcgcccgcg atccggaatt cgttgcccgt
     7201 ctggccgaat acggacgcgg gctggagatc tacctgcagt tcgattccct gcgggccgaa
     7261 gcgcttcaca ccctgcgcgg cgccgatctg acccgcatgc atgcccaggc gctcgaagcc
     7321 ctggaacacc acggcctgtc gacgacactg gtgatgacgc tgcggaacgg ggtgaatgac
     7381 caggagatcg gcgacgtgat ccgccacggg ctgggctatc gctgcgtgcg gggcgtgacc
     7441 ctgcagcccg ttcaggatgc cggccgcaac ctgggcggac ggggggccga agaccggctg
     7501 acggtcagcg ggatccgccg acgcatcgcc gaacagtccg gactattcac cctcgacgac
     7561 atcgtcccgg tcccgtgcaa tccggacacg ctggccatgg gctacgccct ccggctggac
     7621 ggtcaggtgc atccgctcac ccgctggctg gggcccgagg ccctcctcgc cgggcccgcc
     7681 aacaccatcg tcttcgagcg cgatccgtcg atccgggatc gcctgttccg gctcttttcc
     7741 accaatcatt cacccgcgtc cggtgcccgc tgcctgtcgg acctgttgtg ctgcctgccc
     7801 cggatcgacg caccgacgct cgattacacg aatgtcttcc ggatcctcat cgtgcagttc
     7861 atggacgcca ccaacctgga cctgcgggcc ctgaagaaat cctgcgtaca catggtgcag
     7921 ccggacggcc ggatgattcc gttcgaagcc ttcaacctct tctaccgggg ccgcctgacc
     7981 gaagggctgg agaccctccg cgccagcgtt gccgacgaca tcgccaggcg ccaccgatcg
     8041 gtgccctcag cgggctaacg ccgaccccgt ccgttcaagg ctcagcacca gccggcctgc
     8101 gtcgtcgagg agcagcaagg tggccccttc gatacgccag tgtgtgacct gatccagcaa
     8161 agcgtggaag ccgacctctg cgtccatacc ctgaccacag gccatcttcg tgccggcgtg
     8221 cgcactgacg cgcaggtggt cgccgtccac ccggtaggtc ccgatgaacc ggttgcaccc
     8281 tccggttcca gccagacgtc ccgcctccag aaagtgcagc tgaggtatcc ggacgctgcg
     8341 ctgcggatcc accggaaaac catcctgagt gatcaggcgc cagggcgctc cgcgcagacc
     8401 accggaatcg cctgcgaccg gcgggcgtga gctcacgcga cgcagcaaca gcgtcaccgc
     8461 cccatcggga cccccatcaa gcaccggata gacctggtcc gtgacgaacc acggcgcacc
     8521 accgacactg atgcggcccc gcaggctgta gcgcctgtgg gcctcgacat ctgccggatc
     8581 cacctcgacc gcaaagggaa tgggcggcgc accggcgggc tcgaggcgcg tccggccgat
     8641 gaccggtgcc ggcgcatcgg ctcgggacac gtcttccacc gtcacctcga gcacggcctc
     8701 cggaggaagc cgcagacgct cccggtagat ggcagatccg tggatgagct cggcacccac
     8761 gggtaccaca cacaggagca aggccgcagc catcagcagg cgagcacgca tcagggcgct
     8821 ctcaggcagg acttatggac ggggcccatg gacgcagcgc gccgatgcgg tcccccgcat
     8881 ccatcggtca gcccaggttc ttgagggcga attcccaatt gatgagcttg tcgagcaccg
     8941 cattgacgta gtcgccgcgg cgattctggt aatccaggta gtacgcgtgc tcccagacgt
     9001 ccagggtcag aaggggcgtg tagccctggg cctggggcat gtcggcgttg ccggtcttga
     9061 ccaccttgag cgccttgccg tccgccacca gccaggccca gccgctgccg aactgggtca
     9121 gggccgcctg ggcaagttcc tttttcagtg cgtccacact gccgaaggag gcttcgatcc
     9181 gggctttcag ggctgcgggc ggctcgcccc ccccattcgg gcgcaggctg taccagtaga
     9241 aggtgtgatt ccaggcctgc gccgcattgt tgaagatggc ggtcttgtcg gccttgccca
     9301 tgaccttggc gatgatctgc tcgaggctga ggtccgccag ttcgctgccg gcgaccagct
     9361 tgttcagatt gtccacatac gccttgtggt gcttcccgta gtggaagccc agggtgttgg
     9421 ccgagatcac cggcgccagg gcgctgtcgg cgtagggcaa ggcgggcagg acaatgggac
     9481 tggcagcctg ggcggggcgc acgaagccgc cgagtgccaa cgcggacacg gcccccaggg
     9541 aggcaccgag gaactgacgc cggttgaagc cggacgggga atcgaaggtg gaacgggaat
     9601 ccatggggct ctccttgcag gtggggaatg cggatccggt cgggcgaatc cggcttccat
     9661 cattattggc caaggcggac gcccttgtca tggttccctt gcagggtctc aggttggtgc
     9721 gtgaagggtg cgcaagctga atccgagggt ggtgtcgccg gacgaaaacc agatctggcc
     9781 gtcctgcagg gtacaggtga gcgtcatgga acgttccgcg agggccgcca gggcgcggga
     9841 atcatccgtc tcgagggcct ggaccgtcag attgccaagg cgcgcgaggg ccgcgctgtc
     9901 cttttgccac cagatgtcca cggaccggcc cccataggcc agcacgcgga cctgttcggc
     9961 ccgcccgctg gcccggcgca gcagcttctc atccggcagt ccaacctcga tccattccag
    10021 gatgcgcccg gtgtcgtcct tgagccacgc ggccggctca tcctcggagg acaggccgcg
    10081 gccgaactgc aggcgctcat cggcgtacaa cgcaaaggcc aggacgcgca ccatcatgcg
    10141 ttcgtccgtt tcggacggat gccgcgccag ggtcaggacg tgctcgccga aatgaccgcg
    10201 gtccaggtcg gagaccgtca gcgaggcacg gaaaatggtg gatttgagcg ccatgggatc
    10261 aggcgggtgg tcccgctgca atgaaaaacc gggccctacg gcccggttct gttccggtga
    10321 cggaacggat tacttgccga ccgcggcctt ggccttgtcg gcggcttcct tggtggcatc
    10381 ggcagtggcc gcaacagctt ccttgccagc ttccttcgcc gcgccagcag cctccttggc
    10441 agcggcgacc gcatccttgg ccgcgtcctt ggcggcaccg gcggcggccg taccagcttc
    10501 cttggccgca ccggcagcat ccttcgcagc gtccttggtg gcttcggcgg cctgcttggc
    10561 ggcctcttca gccttctggg cggcttcctt ggctgcatcc acgacaggcg cgggggtcgg
    10621 cgcaggggcc tcttccttct tggagcaggc ggcgaggcca agagcgatca gggcggcaag
    10681 ggcaagggca cgagtggtca tctggggttc tccaaatcag cgatttgtat caaaaagtca
    10741 ttgaaaacca taggttttca gtgtgtgcga gaggctaacg cggcaaagtg ccgaaaagat
    10801 gacggatcga caaaaaaagg ccccgaaggg ccttgcacgc ggcgcaacga tcgcgcggat
    10861 cacttcacgc ggttgcggta ctcgtcggtg cgggtgtcga tttcgatctt gtcgccgatt
    10921 tccacgaagg ccggcacgga cagttcaaaa ccgctgggcg cgatgcgggc gggcttcagg
    10981 accttgccgg aggtgtcgcc cttgacggcg ggttcggtgt attcgacttc acggaccaca
    11041 gaggtgggca gatccacgga gatggccttg ccgttgtaga acaccacttc gcagggcatg
    11101 ccgtccacca ggtacttgag ggcgtcggtc atgttctcgg cttcgacctc gaactgctcg
    11161 tagtcggcgt ccatgaacac gtacatcgga tcggcgaagt aggagtaggt gacttccttg
    11221 cgctcgagaa ccacgacttc gaacttgtcg tcagccttgt agacgttttc ggacggggcg
    11281 ccggtcagga ggtttttgag cttcatcttg accacggcag cgttgcggcc ggacttgttg
    11341 tattcggcct tctgcaccac gagggggtcg ttgccgacca tgatcacgtt gccggagcgg
    11401 agttcctgtg cggttttcat gctgtttcct gcttgcgaag attgcgcggc acgtgccacg
    11461 gtaaataggt aacttactat tttagctgat ctttgctgaa ttgcaccaga ccagcggcga
    11521 gttcaccggc ctgttgcagg tgggcgctcc acgccggtgc agccgcggac agcgcgggca
    11581 gggcatccgc aaaggcctgc caggcccggg cggcgtcgtg tccgcggttc cacgcccgcc
    11641 agaaatcccg cacggcagcg ccggccgacg gcgggaggcg ctccgcgtag cgggccagga
    11701 aggcatcgag cttgtccaaa tgggcatcgt cgtcctggcg gtagatctgc cacacgaagg
    11761 ggcgctcggc ccactgggcg cgcacgaagg aatcttcccc ccgcacgaag ttcaggtcgc
    11821 aggcccacag caactcgtcg tagcgggact gctccacgaa tggaatcacg acgacccgca
    11881 gggcaccggc ctcgatccgg tcgccaggct tcagtccatt ccgtcccagg cactgcgcga
    11941 cctgcggcag cacccgccct tcaggcacca ggcaggtgag cggcctggta ccgttccgcc
    12001 agacctccag cagctcgggc agggccgcat tctcgtaggc aaacaaggag atccgcagct
    12061 cgccgggccg cgcggccggc agacccagcc cggtcaggca ctgcacgggt gcgtgggcgg
    12121 cccgacggac gtcgtagtcc cgctcccgca acaggccgcc gctgctggca tcgaagccgg
    12181 gaaagtagaa gtactgggtc aggggcagac gggggtgggg ggaaggcagg cgatggcatc
    12241 cggccaccca gtcttcggcg ctcaggtatt cgagattgat ccacaggggg cggggcaggc
    12301 gcagcgccat cgcttcgaga aagcccgccg gcaggctgca ggcaaaggcg ccaatgacca
    12361 catcgccagg cacgatggcc ggcaaaggat cggtccacgc gcggatctcg acacccgccc
    12421 gatgctggat cgccagcgcc ggttcgaggg acgggatcag acgtcgcgca ctgggcagat
    12481 cgtccaccca caagcgcggc tggacgccat gggccaccgc cagttcccgc gccaggcgcc
    12541 agcacacccc gatgtccccg aagttatcca caactgggca gaagatgtcc cagcagggac
    12601 gggcatcaga tgaggaaccc ggggtggaat tgttcaaggc ggggataaac aggcaaatag
    12661 gacgcggaca gattgtgcac aaaccggcaa tccgaggcga gcccgccttt cctcaacatt
    12721 ctgcccccac gccgtcctgc ccgctgccga cacgtttatc aacatgacaa tcctttgttt
    12781 ccatggggat tattcatgtt gtccactgtt tcgttccgag cgttaacaga agtattgttt
    12841 taataagaaa agtctacaca cttctgatcc catgcgcccc cggcagaacg tcacatggcc
    12901 ggcttacact tgaaacctca cccaggagaa tcccatgctc tcaatcaccg acctgctcga
    12961 tttcattgat ctggaccggg atacggtact gatcgtggct gaagcgaccg gtcttccccc
    13021 cgagccggcc atcatccttg cgcgggaact gctggccagc cgacggggca tcctcaccct
    13081 gcatcacatg ttccgggacg agcgggcccg cgcagatgaa cgtggtcatg tcctgcgggt
    13141 tcgggatctg gatcgcgctt accggcactt tgcccgaaag tacccgcagc ccgtcgtggt
    13201 gtgaacaaac aggggagatt cccgcgcagg aaatgtggac aagtcctcga tggaatggga
    13261 ggcccgattt cctccccaat gcctccccgt ggaccccaca tggctttcca gagctttttc
    13321 aaggatccag tacattgatc ttgctgagga ttcttgggtt atccacagaa tcttccggta
    13381 caccattatt attatcttgt ttacaaacaa gaatattgaa caaccaagat tgccgcgaaa
    13441 tgcgccgtgt cttgcctgtc aggcgtgatc caggacgtcg cccggcggac ggagctgcca
    13501 taatccggtc accctcctgt ctggcccgtc acgcgtgtct gccccgtcct cgcccatatc
    13561 ccgcgctgtc cgccacccct acctcttgct gacgctgacg gtcctgttct ggtccggcaa
    13621 catggtggtc ggtcgtgcct tgcgggaggc cataccaccc atgagtctgg ccctcgggcg
    13681 ctggatcctg gcctttttgc tgaccctgcc tttcgcgtgg ccccatcgga accgcctgca
    13741 gacgctcgac cgccgtgagt ggggcatcct ggcggtcctc ggggtgctgg gcgtcggggc
    13801 ttacaacacc ctggcctaca ttgccctgtc ccacacgacg gcgaccaatg ccgcgctgct
    13861 caactccttc atcccggtgg ccattctggt cctcacctgg tggctgggac atcgcagccg
    13921 gccgctggag atcatcggag tcggggtttc cctggcaggt gtgatgacca tcctcagtca
    13981 cggcgagctc cgggtgctca cccatctgca gctcaacatc ggcgatgtat ggatgcttgg
    14041 cgcggtgctg tcctgggcgc tatacacggt gtgcctgcgc tggcgtccgg aagggctcca
    14101 tcccatggcc ctgctggcgg tgctgaccgc catcgggctg atggtgctgg cgcccctggt
    14161 agcgtgggaa ctggccggcg gtgcgcagat ccggccggac gccggacttc ttgcagggat
    14221 cgcctacacc gggatctttc cggcctttct cggctacgtg ttctacaacc gggccgtcgc
    14281 ccaggtaggc gctgccaccg gcgggctgtt catccacctg atgccggcct tcagcaccct
    14341 gctggcgatg gtcttcctgg gcgagcagcc gcatctgcat cattttttcg gcattgcgtt
    14401 aattctggcg gggatcgggc tgacgacggg tttcaagaac cgctaaactc ggcggcctca
    14461 tctgcggagc tgcgccgtgc gtttcgagca cctcgtcgag atcaacgatc ccctcaaccc
    14521 cctcattccg tccctgacgc gggaacaggt ctggtccggc ctgatgctgc gggtggatga
    14581 gccgacggtc ttccttcccg gtctcgaacg ctgcgaaatc ctgtcgcggg cggccgacca
    14641 ggtcgagcgc cggctccatt tcggcggcgt gcaggtgcgg gacaccgtga ccttcagccc
    14701 gctggagtgg gtgcgcttcg aatcgtccgc caacgcggag cacgcgggcg ggcgcctgac
    14761 gatccgcctc gaacagcccg atcccctgcg tctttacctg cgcttcacct acgagaccac
    14821 gctgccggac ggtggcgcgg ccgatggcgt gcaggtatcg gagttcgtca aatccgccta
    14881 tcgcgaatcg gatgtggata ccgtgcgcat catccgcgtg ctgtccgccg gggggcggcc
    14941 ccactgacgt tgcaggatgc ttccagaaca caccagaaca gaaccaggag acaccatgat
    15001 cgcccgaccc gaactgcgtt ccttccagcc gcccgtgcgc accctgatgg gccccggccc
    15061 gtccgatgtg ccgccgcggg tcctcgccgc cttggcgcgg cccaccgtcg gtcatctgga
    15121 cccggcgttc gtgggcatga tggacgagct caaggaactg ctgcgctaca cctttcagac
    15181 ccgcaatgag ctgactttcc cggtttcggc gccgggctcg gtgggcatgg agacctgtgt
    15241 ggtgaatctg gtcagtcccg gcgatacggt ggtggtgtgc atcaacgggg tctttggcca
    15301 gcgcctcgcc gagaacgtgc ggcgcgccgg tggcgtgccg gtgatcgtcg aggacccgtg
    15361 gggcgagccg gtggatccgg ccaaggtgga agacaccttc aaggcccatc ctgaggcctc
    15421 gctgctggcc ttcgtccatg cggagacctc caccggcgcc ctgtcggatg cggccgcgct
    15481 ggcggccatc gcgcgccgct tcggggccct gacgctgatg gactgcgtca cctccctggg
    15541 cggcgtgccg gtgttgctgg acgaatgggg catcgacgcc gcgtactcgg gtagccagaa
    15601 gtgcctgtcg tgcacgccgg gcctgtcgcc ggtgagtttc tcggaggccg ccgtggcgcg
    15661 catccgtgcc cgcaagacgc cctgccagag ctggttccag gatctgtccc tggtgctcgg
    15721 ctactggagc ggcgcgaagc gcacctacca ccacacggca ccggtcaatc cgctgtacgg
    15781 tctgcacgaa tccctgctga tgctcgccga ggaaggcctg gaggccgcct ggcagcgcca
    15841 ccgggacaac cacctgcgcc tgcgcgccgg tctggaagcc ctgggcctgc gctttgtggt
    15901 gcgggaagac gcccgcctgc cccagctcaa caccgtgtgg gtgcccgacg gcgtggacga
    15961 ggccgccgcg cggacccgcc tgctcaagga atacggtctg gagatcggcg ccggcctcgg
    16021 cgtgttcgcc ggcaaggtgt ggcgcatcgg cctgatgggc cattcgagcc ggccggccaa
    16081 catcgccctg tgtctggcgg cactgaagga cgtgctgggc tgatcaccct gcccatgtct
    16141 tgctgaaagg tgcggcggtg tggccgccct tccggaggcc cagcaccgtg cgacccggat
    16201 gacgcggggt ggtcagcgca gggatcgcac gtagcggtcc ttgtcgccct cgggcgagac
    16261 cgcggggctc cgcaggcgtt cgaagaagcc gttgcgtggc gcaggggcgg gcgtcgatgg
    16321 cgtgtctgcc tcggcggcat ccagatccat ggcatcgcgc aggcgcccgg ccagttccct
    16381 cagctcgtcg tggccgctgg catcggccag ccgcacgatc tcgcgggcgt agtccttgtt
    16441 cgtgcgcatg tattccacgt cggtgatgcg gttggtgacg cgacgcatca gcagatggat
    16501 gcgcccgagc aggccgtaga ggacagaatc ggattccatg ggatctcccg gaggcgagcg
    16561 gttcagtcga gggcttcgcc gtgctcggcg atgtcgaggc cgtccgcctc ggtggtggcg
    16621 tccacccgca gtccggtgat cacctggacg atccccagca cgatgagggt catcagcgca
    16681 ctgtaggcga ccgtggcgcc cacgccggcc agctgggcgg acatccggcc cgcgctgccg
    16741 ccgatggccg gcgaggccag caggccggtg agcagtgccc cgatgatgcc gccgacgccg
    16801 tggatgccga aggcgtcgag ggcgtcgtca tagcccagca tgcgtttcag gctggtcgac
    16861 gcgatgtagc agccggcacc tgccgccagt ccgatcagaa gggccgacga gggcgacaca
    16921 tagccggcgg ccggtgtgat ggctaccaga ccgcccacgg cgccggagac aaggcccagg
    16981 gcgctgggtc gtccgcgcag catccattcg acagccatcc aggtcaccgc ggcggtggcg
    17041 gcagccatct gggtgaccag catggccatg ccggcgtgac tgtcggcggc cagggccgaa
    17101 ccggcattga atccatacca gcccacccac agcagggcgg cgccgctcag cgtgagggtc
    17161 aggttgtggg gcgtcatcgg ctcgcgtccg aatccctggc gagggccgag tacgagcgca
    17221 cacaccagcc ctgccacgcc ggcgttgatg tgcaccacgg tgccgcctgc ataatcgagg
    17281 gcacccagcg cagccagcca gccgcccggt tcccacaccc agtgggcgac cggcgcgtag
    17341 accagcacgg accacagggc ggcgaagagc agcacggctg aaaatttcat ccgctccgca
    17401 aaggcaccgg gcaccagcgc ggtggtgatg atcgcaaaag tgagctggaa catgacatag
    17461 accgtttccg gcacggcgcc gcccgcaagg ccctccgcct gcagcccttc gaggaacagg
    17521 cggtccagcc cgcccaggaa aggcgtgccc ggccgcaggg ccaggctgta gccggccacc
    17581 atccaggtga gggtgaccac tgcggcgatg gcaatggtct gggtcatggt ggacaggacg
    17641 ttcttgctcc gcaccatccc cccgtagaag agggccaccc ccggcagact catgagcagg
    17701 accagggcgg tcgccgtgag catccaggcg gtgttacccc cctccgcatg ggcagcgggc
    17761 gcgctctggg ccagtgccgg accggaaagg gcgagcagcg cggccatgcc cagggacagg
    17821 ttgaccgggc ggtttgacgg gactatccgg cctggggccg atggtcgggt ggacgggtgt
    17881 gcgggcatga catcctctct gcagggtatg acgcttctgt tgagcgtttg tgcacccgca
    17941 gcaagagccg tgccgcgctg aaattctgca aattacggtg tccttggcgt ccggaacgca
    18001 cccgaaacgg gagcgtcatg ctttccggca cgcagacggt gcatggagcg gtaaccgccc
    18061 ggtccacgaa aaagcccggc cgaagccggg cttggggtgc tgccgaaagt ggcgccgacg
    18121 atcgtgctca ggcggccgcc ttttcctcgc cggagacgaa gaagctggcg acgtagatca
    18181 gcagacccac caggaagacc acgccggtgc cttcgcgcag ccagtagaac agggagatct
    18241 gttcctgggc ggccatgaag gggatgggcg agctgctgaa gcgttgcagc cacacctgca
    18301 ggatgcc
//
LOCUS       DAOQMM010000073        18030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1154_length_18030_cov_52.357997, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000073 DAOQMM010000000
VERSION     DAOQMM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1154_length_18030_cov_52.357997"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /gene="aspS"
                     /locus_tag="PLH85_11965"
     CDS             <1..1403
                     /gene="aspS"
                     /locus_tag="PLH85_11965"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNB65326.1"
                     /translation="QKNMILRYRTAMAFRRFLDAQGFIDIETPMLTKSTPEGARDYLV
                     PSRVHDGQFFALPQSPQLFKQMLMVAGYDRYYQITKCFRDEDLRADRQPEFTQVDIET
                     SFLTEADITAMMEEMIRTVFQEVLSVELPAPFPRMTYAEAMDRFGSDKPDLRVTLELT
                     EVTDLVKDVAFKVFSGPANSGGRVAAMRVPGGAAGDRVLSRGEIDEYTKFVGIYGAKG
                     LAYIKVNDASQPNESGLQSPIVKNLHETALRAILERTGAQSGDLIFFGADKTKVVNDA
                     LGALRIKLGHEKGYVNGKAWEPLWVVDFPMFEYDEEDKRWTACHHPFTSPKDEHLALL
                     ASDPGKCLAKAYDLALNGWEIGGGSVRIHRADVQEKVFTALNIGPEEQQLKFGFLLDA
                     LKYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKTQRAQCLLTNAPSPVDEKQLRE
                     LHIRLRNIKAPDVPAA"
     gene            1533..3149
                     /locus_tag="PLH85_11970"
     CDS             1533..3149
                     /locus_tag="PLH85_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB65327.1"
                     /translation="MIDLDTITALVVEANPTMRTQLRNMLNLSGISKVQFAVTAGSAV
                     RKLRDNRFDLILCEYHLGEGQDGQHLLEDLRLHHIIPLSTLFIMVTGERQYERVVSAA
                     ELAPNDYILKPFAADTLQERIERALTKRDAFMPAYRLIELGNAPDAIAVCAAGENDHP
                     QWAVDFMRLRAELLLTCGKADDAHKLYSKILSLRSIPWARLGLAKALFLQKRFAEAES
                     LLQGLVGENDMYLDAYDWLSRTREAAGELEAACKVLATATAVSPHRVGRLRKLGELAI
                     ETGDHDTAEKVLSEVVRKGKYSDFRDPEDHVRLLQAQLAKGDTAQAESTIRDLERTMP
                     GLDKTRPCATLSSALLHLKKGNADKAGEALRALTSEGSNAHDLSVGLKRELAKACFAS
                     QLEDHGSEVVLDLMRNAADERSMERAKAVLRDAGKAELGETLAAQMQMEVRDLVSEGA
                     RKAQEGDFDGAVQLMMNAVRKMPGNVHVLFNATLALLKHVENCGWNEQFAHQARDLME
                     RARGQDPGNARLPALTAYYYTLLKKYGIQP"
     gene            3210..3605
                     /locus_tag="PLH85_11975"
     CDS             3210..3605
                     /locus_tag="PLH85_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNB65328.1"
                     /translation="MSRLLPLFRCLLVLLCLIGSSAATARSPWDDLIGSRADRATIPL
                     RALPPEARDTLARIRAGGPFPFRRDGIAFQNRERRLPAAEPGFYREYTVITPGADGRG
                     ARRIISGGWPPTIYYYTDDHYRSFRRIAE"
     gene            3656..4036
                     /locus_tag="PLH85_11980"
     CDS             3656..4036
                     /locus_tag="PLH85_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNB65329.1"
                     /translation="MFTPEFDSDDDETVDTPLIRIDLEGVTDKAGFLAAVAAALHFPA
                     HFGHNWDALADCLTDLSWLPRAPGYAIAFDHADAFAAAAPEAFATALSIFQEAADFWR
                     AEGVALWTLVGSTQTVTPDFQDPT"
     gene            4033..4512
                     /gene="nudB"
                     /locus_tag="PLH85_11985"
     CDS             4033..4512
                     /gene="nudB"
                     /locus_tag="PLH85_11985"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNB65330.1"
                     /translation="MSGIKQPVSVLVVIHTPALDVLLLERAGPSGLWQSVTGSREGDE
                     TLAATARREVAEETGLLLPDAANGLRDLRLANRFPILPRWRARYPEGVTHNTEHVFSL
                     CIPEPVPVRLAPDEHVAARWLPWQDAAAQVFSWTNRDAIRLVADRLRADPAAPPASA"
     gene            complement(4475..4612)
                     /locus_tag="PLH85_11990"
     CDS             complement(4475..4612)
                     /locus_tag="PLH85_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65331.1"
                     /translation="MTAGDTTCWCASLPPLLAVPTAPTTAACYCPACLRRMLAAQPDQ
                     P"
     gene            4807..5223
                     /locus_tag="PLH85_11995"
     CDS             4807..5223
                     /locus_tag="PLH85_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB65332.1"
                     /translation="MSTLTRRDPLDDFFRGFFVRPVDFAGSPAEAPQMRVDVKETPEG
                     YQVHAELPGVKKEDIHIHIDGPVVSISAERKQDKEVKEGERILRTERYFGQVSRSFQL
                     GQDVDEAAANARFNDGVLELTLPKKVAPKAKRLIIE"
     gene            5333..6232
                     /gene="argB"
                     /locus_tag="PLH85_12000"
     CDS             5333..6232
                     /gene="argB"
                     /locus_tag="PLH85_12000"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNB65333.1"
                     /translation="MTQPSLTADLSPAQKAAIIAEALPYIRRFQDKTLVIKYGGNAMT
                     DPQLKACFAHDVVLLKLVGFNPVVVHGGGPQIENMLAKVGKKGEFIQGMRVTDAETME
                     LVEMVLGGQVNKEIVSLINQAGGKAVGLTGKDSNFIRAKKLMMENKDVPGDLIDIGQV
                     GDITQIDPSLISFLDKGDFIPVIAPIGVGEDGESYNINADVVAGKLAEILKAEKLVLL
                     TNTPGVLDKSGKLLTGLTPRQIDDLVADGTLSGGMLPKIGSALDAARNGVKSVHIIDG
                     RVEHCLLLEILTDHGVGTMIKSK"
     gene            6314..6826
                     /locus_tag="PLH85_12005"
     CDS             6314..6826
                     /locus_tag="PLH85_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNB65334.1"
                     /translation="MHSTLARLPLRVLALALFAVCGALLAFGLYLQHVKGIEPCPMCI
                     MQRYALLGCGLIGLLAGLHAPGPGGTRAYAGLLMLTALAGGGVAARQSWIQWFPPAVS
                     ECGPGLEYMLESFPLSSALPMIFRGAGDCSLVDWTFLGLSIANWSFLCFAVILLAALA
                     VLTRRSTPGA"
     gene            7167..9629
                     /gene="nirB"
                     /locus_tag="PLH85_12010"
     CDS             7167..9629
                     /gene="nirB"
                     /locus_tag="PLH85_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNB65335.1"
                     /translation="MRKLKLVMVGNGMAGVRTLEELRKLAPDMYDITVFGAEPHPNYN
                     RILLSPVLAGEMTIQDIILNDLQWYADNGITLHLGSKVTSIDRQNRSVVATDRDGQTI
                     TVDYDRLLLATGSNPFILPIPGNDLPGVISYRDIKDTDEMIDAAAKYKHAVVIGAGLL
                     GLEAANGLALRGMDVTVVHISDWIMERQLDRTAGKMLQKALEEKGMKFKLNAQTAELV
                     RGESGRVCAIKFKDGETLPADLVCMAAGIRPNVDLAEKAGIHCNRGIIVNDTLQTYDP
                     AIYAVGECANHRGTAYGLVAPLFEQAKVCANHLAEIGIARYEGSVTSTKLKVTGIDVF
                     SAGDFSGSEGTEDIVLHDPGLGVYKRIVLKDDKLVGAVMYGDTKDGAWYFQLLKDEQN
                     IHDIRDHLVFGNAHLGDVGHKGTNVAASMPDTAEVCGCNGVCKGTIVKAIQEHGLFTL
                     EDVRKHTKASSSCGSCTGLVEQILASTIGGAYEPADSANKPVCGCTDHSHGDVRRAIR
                     EHKLLSVGDTQKFLEWKTPNGCATCRPALNYYCISTWPHEALDDPQSRFINERAHANI
                     QKDGTYSVVPRMWGGLTTPGELRAIADAAEKYKVPTVKVTGGQRIDLLGVKKEDLPAM
                     WADFAQAGMVSGHAYGKSLRTVKTCVGAEHCRFGTQRSMDLGVKLEKMLFGMWSPHKV
                     KLAVSGCPRNCAESGIKDVGVIGVDSGYELYIGGNGGIKTEVAQFFCKVTNDDEVMEY
                     GGAFIQLYREEGFYLERTCHYLERVGMEHIKKEVLEDADKRKALYDKLLFALQNYKDP
                     WTEIVEDRSGGEFKRQFEIIKV"
     gene            9639..9950
                     /gene="nirD"
                     /locus_tag="PLH85_12015"
     CDS             9639..9950
                     /gene="nirD"
                     /locus_tag="PLH85_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNB65336.1"
                     /translation="MSNWKKICAVSDVPVLGSRVVHAEEVGIIAVFRNSADEIFAVRD
                     KCPHKGGPLSQGIVHGSTVTCPLHSWKIQLEDGQVVPPDVGCVKKFPVKVENGEVFLG
                     L"
     gene            10096..11310
                     /locus_tag="PLH85_12020"
     CDS             10096..11310
                     /locus_tag="PLH85_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNB65337.1"
                     /translation="MDRKSFLQAGHTPTLIAAFLYFDLAFMVWVLLGPLGVQIAKDLG
                     LSHAEKGLMVATPVLAGAALRIVMGVLVDHLKPKTAGAIGQVIVIGALAAAWKLGIHN
                     YEETLILGFFLGVAGAAFAVALPLASRWYPPEHQGTALGIAGAGNSGTALAALFGPGL
                     AAAYGWNNVFGLALIPLSIVFVLYLFMAKDAPECPPAKSLTEYLAVLKDKDAWWFMFF
                     YSVTFGGFVGLASSLTIYFNTQYGLDPKTAGFYTAACVFAGSLVRPIGGNVADRIGGI
                     KSLTVMYVLAAIFLAIVSFGMPESWQALLVFVCAMLSLGMGNGAVFQLVPQRFRKEIG
                     VMTGLVGMAGGVGGFYLASSLGYSKQVTGSYQSGFLIFAALAIAALVGLTAVKSRWRT
                     TWGAPHLTSAKI"
     gene            11505..13232
                     /locus_tag="PLH85_12025"
     CDS             11505..13232
                     /locus_tag="PLH85_12025"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HNB65338.1"
                     /translation="MTAATTAPKNARLQARIGHASLTGPRPRNEDFCGAVTPDGAELD
                     TKGILAVVADGVGGHANGREASEYTVRGLLSDYYATPDTWAVNKALDTVLNALNRWLV
                     SQASRTREAAGMATTLSAIVVRGRRFYTAHVGDSRIYRLRDGRFEQLTTDHLWEHPEL
                     KNVLSRAIGLDPHLSVDYADGDLEPGDVFALMSDGVWNQLGDKRIADILRSQDEPQAA
                     AARLAGEAEDGGSQDNCTALVVRVDALPPDRLRDTIARLGQLTLPPRLKPGQELDGLT
                     VDALLHESIATLLYRVHDAQGHPRVLKTLRPERNDPEASAALAHEEWLARRVNDAYFP
                     QVILHPARAHLYYLMSWHEGATLKARLESGHRFGLGEIVSLGERLLKGLAGLHRLSII
                     HRDIKPDNLHLDNDGRLRILDLGVAASDSRGDGQQFHEINNPGTPSYMAPELYGPTPT
                     AANEQTDLYAVGVTLYELLTRKYPYGEIEPFQNPKFGDPVPPTRFRPDIPAWMEAVLL
                     KAVARDPRARFETAEEFLLALERGAHRPLVVPRKSPLLQRDPQLGLKLLAAASLTLNI
                     LLIYLLLVR"
     gene            13285..15525
                     /locus_tag="PLH85_12030"
     CDS             13285..15525
                     /locus_tag="PLH85_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65339.1"
                     /translation="MMHTPDPSQPPPPLFDRENPGQLLRTMGGISLLLTLIVGVSVFA
                     QHDVSPIHGLTALGVCIALCAGLLGFRRHPDRLPSRCVLWGAWLIILIGAAFTGGNRT
                     TSLSAIPVLIVLVGWLFGMRHALLMTAVSILALLGMSLSEQYDWLPMGGSSPPLSRWM
                     VLSNVLLLTAFLTLHSRRLYLSRYQALHRLSATLGLLTEGTPIMLAAVDGRGCYSYVN
                     QNYAHFHGLAPTALRGSLVDAVLGDGIQQHNLARLDAGGGSATFRSRRHNRQTGDERW
                     IETCLRSATATDGRTDGYYALLRDITDEVRTEEEIRFLAYHDALTGLPNRILLADRLR
                     QAIARAARDDTLVAVCYLDLDRFKFFNDTWGHTVGDEILVQVAGRLRDSLRGSDTVAR
                     LGGDEFLVLIGGIADRQEMPAALARLLGTVSQPVVLEDGAEIRISASAGVALFPRDGE
                     EPDVLLRHADQAMLLAKQSGRARYALFDTELEQRNHHLQRIVSSVDRGLDGGQFRLFF
                     QPKVNLRLSSVIGAEALIRWEHPDEGLLLPDAFLPWLESSPVGVRLDRWVLAAALDQM
                     SRWHAAGLDIPVSINVSGHHLLAPDFVDHLTAQLIAHPSVAPERLELEILETTAMEDV
                     DQVARVIRACTALGVSFALDDFGTGYSSLTYFRRLPARTLKIDRSFVRDILADPEDLA
                     IVKGVVALARSFGRQVIAEGVETPEHCGPLLSLGCDCVQGYGIGHPMPAAEFPGWVRT
                     WKSVNG"
     gene            15596..16795
                     /locus_tag="PLH85_12035"
     CDS             15596..16795
                     /locus_tag="PLH85_12035"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNB65340.1"
                     /translation="MHYGEFDTASLMFITPRPELIFVRGDGSWLHDDTGKRYLDFVQG
                     WAVNCLGHCPAEIREALADQAARLINPSPAFFNGPMTELAGLLTEHSCFDRVFFANTG
                     AEANEGAIKLARKWGRMHKDGAYEIITFEHSFHGRTLATMSASGKPGWDTLFAPQVPG
                     FPKARLNDLDSVGALIGDKTVAIMLEPVQGEGGVIPAEPGFLQALRDLTRAHGLLLIV
                     DEVQTGMGRTGRLFAHEHAGIEPDIMTLGKGIGGGVPLSALLAKEAVCCFQPGDQGGT
                     YNGNPLMTAVGAAVLRRLLSDGFLARVDTLGRYLESRLQALVTRHALAGERGAGLLRA
                     LILDSDRGPALVKAALDYAPEGLLLNAPRPNLLRFMPALTVTEAEIDRMIEILDTLLG
                     AVRADAG"
     gene            17069..>18030
                     /gene="ychF"
                     /locus_tag="PLH85_12040"
     CDS             17069..>18030
                     /gene="ychF"
                     /locus_tag="PLH85_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNB65341.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     EVPDPRLAALSEIVKPQKVQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDENVVHVNGKVDPLADIETIVTELALADMSAVEKAIHRENKKARAGDKDAQK
                     LVALLERLMPHLNEGQPVRTLSLSDDEKQLLKPLCLMTLKPAMYVGNVLEDGFENNPY
                     LERLREHAAEEGAPVVALCAKIEAELADLDDADKLAFLADLGLDEPGLNRLIRAGYKL
                     LGLQTYFTAGVKEVRAWTIHVGDTAPQAAGVIHTDFERGFIRAQT"
BASE COUNT         3068 a         6820 c         5328 g         2814 t
ORIGIN      
        1 tgcagaagaa catgatcctg cgctaccgca cggccatggc cttccgccgc ttcctggacg
       61 cccagggctt catcgacatc gagacgccca tgctcaccaa gagcaccccc gaaggcgccc
      121 gcgactacct ggtcccgtcc cgcgtgcacg atggccagtt cttcgccctg ccccagtccc
      181 cgcagctctt caagcagatg ctgatggtcg ccggctacga ccgctactac cagatcacca
      241 agtgcttccg ggacgaagac ctgcgtgccg accgccagcc cgaattcacc caggtcgata
      301 tcgagacctc cttcctcacc gaggccgaca tcacggcgat gatggaagag atgatccgca
      361 ccgtcttcca ggaagtgctg tccgtcgaac tgcctgcgcc tttcccgcgc atgacctacg
      421 ccgaagccat ggaccgcttc ggctccgaca agcccgatct gcgcgtcacg ctggaactca
      481 ccgaggtcac cgacctggtc aaggacgtgg ccttcaaggt cttctccggc ccggccaact
      541 ccggcggtcg cgtggccgcc atgcgcgtgc cgggaggcgc cgcgggcgac cgcgtgctga
      601 gccgcggcga gatcgacgaa tacaccaagt ttgtcggcat ctatggcgcc aagggcctcg
      661 cctacatcaa ggtcaacgac gccagccagc ccaatgagag cggcctccag tcgcccatcg
      721 tcaagaacct tcacgaaacc gccctgcgcg ccatcctgga gcgcaccggc gcccagtccg
      781 gcgacctgat cttcttcggc gccgacaaga ccaaggtcgt caatgacgcc ctcggcgccc
      841 tgcgcatcaa gctcggccac gaaaagggct acgtcaacgg caaggcctgg gaacccctgt
      901 gggtcgtgga cttccccatg ttcgagtacg acgaggaaga caagcgctgg accgcctgcc
      961 accacccctt caccagcccc aaggacgagc acctcgcact gctcgccagc gatccgggca
     1021 agtgcctcgc caaggcctac gacctggccc tcaacggctg ggagatcggg ggcggctccg
     1081 tgcgtattca ccgggccgac gtgcaggaaa aggtcttcac cgccctcaac atcgggccgg
     1141 aggagcagca gctcaagttc ggcttcctgc tggacgccct caagtacggc gcgccccccc
     1201 acggcggcct cgccttcggc cttgaccgca tcgtcaccat gatgaccggc gccgagtcca
     1261 tccgcgacgt gatcgccttc cccaagaccc agcgcgccca gtgcctgctc accaacgccc
     1321 cgtctcctgt ggacgagaag cagctgcgcg aactgcacat ccgcctgcgc aacatcaagg
     1381 cgccggacgt gcccgccgcg taagcgccgg caagaccgtc tccgccaggg cgaacgggac
     1441 atcccgttcg ccctttttcg tggacgtcgc actcaagaaa ccccggaccg ggccgatgca
     1501 ttcatcgttc ccgcgtctgc ccggctgccc ccgtgatcga tctcgacacc atcactgccc
     1561 ttgtcgtcga agccaatccc accatgcgga cgcagcttcg caacatgctc aacctgagcg
     1621 gcatcagcaa ggtccagttc gccgtcaccg cgggctccgc agtacgcaag ctgcgggaca
     1681 accgcttcga cctgatcctc tgcgaatacc acctgggcga agggcaggac ggccaacacc
     1741 tgctcgaaga cctgcgcctc caccacatca tccccctgtc gaccctgttc atcatggtca
     1801 ccggcgaacg ccagtacgaa cgggttgtca gcgccgccga gctcgccccc aacgactaca
     1861 tcctcaagcc cttcgccgcc gacaccctgc aggagcgcat cgagcgggcc ctcaccaagc
     1921 gcgacgcctt catgccggcc tatcgcctga tcgaactggg caacgccccg gatgccattg
     1981 ccgtctgcgc cgcgggcgaa aacgatcatc cccagtgggc ggtggacttc atgcgcctgc
     2041 gcgccgaact gcttctgacc tgcggcaagg ccgacgacgc ccacaagctg tactcgaaga
     2101 tcctcagcct gcgcagcatt ccgtgggccc gcctgggcct cgccaaggcc ctcttcctgc
     2161 agaagcggtt cgctgaagcc gaaagcctgt tgcagggcct ggtcggcgaa aacgacatgt
     2221 acctggacgc ctacgactgg ctgtcccgca cccgcgaagc cgccggcgaa ctggaagccg
     2281 cgtgcaaggt gctcgccacc gccaccgcgg tgtcgcccca ccgggtcggc cgactgcgca
     2341 agctcggcga gctggccatc gagaccggcg accacgacac cgccgagaag gtgctgtccg
     2401 aagtggtgcg caagggcaag tattccgact tccgggaccc ggaagaccac gtccgcctgc
     2461 tccaggctca gctcgccaag ggcgacacgg cccaggccga gtccaccatc cgggatctgg
     2521 agcgcaccat gcccggcttg gacaagaccc gaccctgcgc cacgctttcg agcgccctcc
     2581 tgcacctcaa gaaaggcaac gccgacaagg ccggcgaagc cctgcgtgcc ctcacgtcgg
     2641 agggcagcaa tgcccacgac ctgtccgtcg gcctcaagcg ggaactcgcc aaggcctgct
     2701 ttgcgagtca gctcgaggac catggcagcg aggtcgtcct cgatctcatg cgcaatgccg
     2761 ccgacgaacg cagcatggag cgcgccaagg cggtgctgcg ggacgcgggc aaggccgagc
     2821 tgggtgaaac cctcgctgcg cagatgcaga tggaggttcg cgacctcgtc agcgaaggtg
     2881 cccgcaaagc ccaggaaggc gacttcgatg gcgccgtgca gctcatgatg aacgccgtac
     2941 gcaagatgcc gggcaacgtc cacgtcctgt tcaacgccac cctggccctc ctcaagcacg
     3001 tggaaaactg cggctggaat gagcagttcg cccatcaggc gcgggatctc atggaacgtg
     3061 cccgcggtca ggatccaggc aacgcgcggc tgcccgccct gacggcctat tactacaccc
     3121 tgctcaagaa atacggcatc cagccctgag gcgcggtcag cgcctgcggc ctgctaaact
     3181 gccgccaccc ccattcccgt ccgtccccga tgtcccgact gttgcccctg ttccggtgcc
     3241 tgcttgtcct gctgtgcctg atcggctcat ccgcggccac cgcacggtca ccctgggacg
     3301 acctgatcgg atcgcgcgcg gaccgcgcaa cgatcccgct tcgtgccctg ccgccggaag
     3361 cccgcgacac cctcgcacgt atccgggccg gcggaccgtt tcccttccgc cgcgacggta
     3421 tcgccttcca gaatcgggag cgccgcctgc ccgccgccga acccggcttc taccgcgaat
     3481 atacggtgat cacccccggc gccgacggcc gtggcgcccg tcgcatcatc tcgggcggat
     3541 ggccgcccac gatctactac tacaccgatg accactaccg cagcttccgc cgcatcgccg
     3601 aatgaccggc ccgcggacag cccgcgcccg ctcacccggc acctggacac ctcccatgtt
     3661 cacccctgaa ttcgattccg acgacgacga gacggtcgac acccccctga tccgcatcga
     3721 cctggagggc gtcaccgaca aggccggctt tcttgccgcc gtggccgcag ccctgcattt
     3781 tcccgcccac ttcggccaca actgggacgc cctggcggat tgcctcaccg acctgtcctg
     3841 gctgccgcga gcccccggct acgccatcgc cttcgaccac gccgacgcct tcgccgctgc
     3901 ggcgccggag gccttcgcca ccgccctgtc catcttccag gaggcggccg atttctggcg
     3961 ggcggaaggc gtcgccctgt ggaccctggt gggctccacc cagaccgtca cgccggactt
     4021 ccaggacccg acatgagcgg catcaagcag cccgtctcgg tgctggtggt gatccatacc
     4081 ccggccctgg acgtcctgct cctcgaacgc gccggcccgt ccggcctgtg gcaatcggtc
     4141 accggaagcc gcgaggggga cgaaaccctg gcggccactg cccgccggga agtggcggag
     4201 gagaccggtc tcctgctccc cgacgctgcg aacggcctgc gcgacctgcg cctcgccaac
     4261 cgcttcccca tcctgccccg ctggcgtgcc cgttatccgg agggcgtcac ccacaacacc
     4321 gagcacgtct tcagcctgtg cattccggaa cccgtgccgg tgcgactcgc cccggacgag
     4381 cacgtggccg cccggtggct gccctggcag gacgccgccg cgcaggtgtt ctcgtggacc
     4441 aaccgggacg ccatccgcct cgtggccgat cgcctcaggg ctgatccggc tgcgccgcca
     4501 gcatccgcct gaggcaggcc gggcagtagc aggcggccgt cgtcggggcc gtcggcaccg
     4561 ccagcagggg cggcagggat gcacaccagc acgtcgtgtc gccggccgtc atcccgcagg
     4621 tgaagcgcgt accacaggcg gggcacaggt tggggggttc cttcgtctgc tccatcgtgc
     4681 tcaatccgaa acgtccagcc ggcagtctag gggcttgccg gcccttgaag ccagcatccc
     4741 gatccccata ttggatacaa ggcgggcaga tcgcccgcga cgctcactga cgaaaggaga
     4801 acccgcatga gcaccctcac tcgccgcgac cctctggacg actttttccg cggctttttc
     4861 gtccgccccg tggacttcgc cggcagcccc gccgaagcgc cccagatgcg tgtggacgtg
     4921 aaggaaaccc cggaaggcta tcaggtgcat gccgaactgc cgggcgtgaa gaaggaagac
     4981 atccacatcc acatcgacgg cccggtggtg tccatcagcg ccgagcgcaa gcaggacaag
     5041 gaggtgaagg aaggggaacg catcctgcgc accgaacgct acttcggcca ggtttcgcgc
     5101 agcttccagc tcggtcagga cgtggatgaa gctgcggcca atgcccgctt caacgacggt
     5161 gtcctcgaac tgacgctgcc caagaaggtg gcgcccaagg ccaaacggct gatcatcgaa
     5221 taagccccca cgccacatcc ccggcgggaa gagcgatctt cccgcttttt ctttttgttt
     5281 gccgtccccg tcattacaat cgcgcctacc cgcccactac cgcgctcgca ccatgaccca
     5341 gccctccctc accgccgacc tcagcccggc ccagaaggcc gccatcatcg ccgaggccct
     5401 gccttacatc cgccgcttcc aggacaaaac cctggtcatc aagtacggcg gcaatgcgat
     5461 gaccgacccg cagctcaagg cctgctttgc ccacgacgtg gtgctcctca agctggtggg
     5521 cttcaacccg gtggtcgtcc atggcggggg accgcagatc gagaacatgc tcgccaaggt
     5581 gggcaagaag ggcgaattca tccagggcat gcgtgtcacc gacgccgaga ccatggagct
     5641 ggtcgagatg gtgctgggcg gtcaggtgaa caaggagatc gtgagcctca tcaatcaggc
     5701 tggcggcaag gccgtgggcc tcaccggcaa ggactccaac ttcatccgcg ccaagaagct
     5761 gatgatggag aacaaggacg tgccgggcga cctgatcgac atcggccagg tgggtgacat
     5821 cacccagatc gacccgagcc tgatctcctt cctcgacaag ggcgacttca tcccggtgat
     5881 cgcccccatc ggcgtcggcg aagacggcga gagctacaac atcaacgccg acgtggtggc
     5941 cggcaagctg gccgagatcc tcaaggccga gaagctggtg ctgctcacca acaccccggg
     6001 cgtgctggac aagtccggca agctgctcac cggcctcacc ccgcgccaga tcgacgatct
     6061 ggtggccgac ggcaccctgt ccggcggcat gctgcccaag atcggttctg ccctcgacgc
     6121 cgcccgtaac ggcgtgaaga gcgtgcacat catcgacggc cgcgtcgaac actgcctgct
     6181 cctcgaaatc ctcaccgacc acggcgtcgg cacgatgatc aagagcaaat aaggcctgag
     6241 ggccggggaa cgcgccctgc cttccccggt ccaccgcagg cccccatcgc cggcgccccc
     6301 tgccgaatct cccatgcatt ccacgctcgc ccgcctgccc ctccgcgtcc tggccctcgc
     6361 cctgttcgca gtctgcggcg ccctgctcgc cttcggcttg tacctccagc acgtcaaggg
     6421 catcgagccc tgtcccatgt gcatcatgca gcgctatgcc ctgctcggct gcggcctcat
     6481 cgggctgttg gcgggcctgc acgcgccggg ccccggcggc acacgtgcct acgcaggcct
     6541 cctcatgctc acggcactgg ccggcggcgg ggtggcggcc cgccagtcat ggatccagtg
     6601 gtttccgccg gcggtctccg aatgtggtcc gggtctggaa tacatgctgg agagcttccc
     6661 gctgtcgtcg gcgctgccca tgatctttcg cggcgccggt gactgctcgc tggtggactg
     6721 gaccttcctg ggcctgtcca tcgccaattg gtccttcctg tgcttcgcgg tcatcctcct
     6781 ggccgccctt gccgtcctga cgcgtcgcag cacacccggc gcctgaaacg cgccccacgg
     6841 cggagcagtg cgcaccagcg cagtgcgatc cgcagcgcgc gtctgccgcc tgaaatgccc
     6901 gaatcccgcg gcccacgcgg ggggcgcgcc ctggcatgac cgttgctttt atttctccaa
     6961 cgcaagctgc gcacggcaat aacggcattg ccgcagtgca gcccccctga tccaggtgtt
     7021 tgcgcgcccc actgcccggg gcgacgtggc aaggccaacg gaggtcccgc cacgatcgac
     7081 gacgatctcc cctgcccaca ggctttacgc ccgcatcggc cgccctgcgc gaaccggtcc
     7141 ggggagattc aatcgtcgga ggaatcatgc gcaaactcaa actggtcatg gtcggcaacg
     7201 gtatggcggg cgtccgcacc ctggaagaac tgcggaagct ggccccggac atgtacgaca
     7261 tcacggtctt cggagccgag ccccacccca actacaaccg tatcctgctc tccccggtgc
     7321 tcgccggcga gatgaccatt caagacatca tcctgaatga cctgcagtgg tatgccgaca
     7381 acggcatcac cctgcacctg ggcagcaagg tcacgtcgat cgaccgccag aaccgctcgg
     7441 tggtcgccac cgaccgcgac ggccagacca tcaccgtcga ttacgaccgg ctgctgctgg
     7501 ccaccggctc caatcccttc atcctcccca tccccggcaa cgacctgccg ggcgtcatct
     7561 cgtatcggga catcaaggac accgacgaga tgatcgacgc tgcggccaaa tacaagcacg
     7621 cggtggtgat cggcgccggc ctgctcggcc tggaagcggc caacggcctg gccctgcgcg
     7681 gcatggacgt gaccgtggtg cacatctccg actggatcat ggaacgccag cttgaccgca
     7741 ccgccggcaa gatgctgcag aaggccctcg aagaaaaggg catgaagttc aagctcaacg
     7801 cccagaccgc agaactggta cgcggggaat ccggccgggt gtgcgccatc aagttcaagg
     7861 acggcgaaac cctcccggcc gacctggtgt gcatggccgc cggcatccgc cccaacgtgg
     7921 atctggcgga aaaggccggc atccactgca accgcggcat catcgtcaac gacaccctgc
     7981 agacctacga cccggccatc tacgcggtgg gcgaatgcgc caaccaccgc ggcactgcct
     8041 acggcctcgt cgcgcccctg ttcgaacagg ccaaggtgtg cgccaaccat ctggccgaga
     8101 tcggcatcgc ccgttacgaa ggctccgtca ccagcaccaa gctcaaggtg accggcatcg
     8161 acgtgttctc cgccggcgac ttttccggca gcgaaggcac cgaggacatc gtcctccacg
     8221 atccgggcct gggcgtgtac aagcgcatcg tcctcaagga cgacaagctg gtgggcgccg
     8281 tgatgtacgg cgacaccaag gatggtgcct ggtacttcca gctcctcaag gacgagcaga
     8341 acatccacga catccgcgac cacctggtct tcggcaacgc ccacctgggc gacgtgggcc
     8401 acaagggcac caacgtggcc gccagcatgc ccgacaccgc cgaagtgtgc ggctgcaacg
     8461 gcgtatgcaa gggcaccatc gtcaaggcca tccaggagca cggcctcttc acgctggaag
     8521 acgtgcgcaa gcacaccaag gcctccagct cctgcggctc ctgtaccggt ctggtggaac
     8581 agatcctcgc ctccaccatc ggcggcgcct acgagccggc cgactccgcc aacaagccgg
     8641 tatgcggctg caccgaccat tcccatggtg acgtgcgcag ggcgatccgc gagcacaagc
     8701 tgctgtccgt cggcgacacg cagaaattcc tcgaatggaa gacgcccaac ggctgcgcca
     8761 cctgccggcc ggccctgaac tactactgca tctccacgtg gccccatgaa gccctggacg
     8821 atccccagag ccgcttcatc aacgagcgtg cccacgccaa catccagaag gacggcacct
     8881 actccgtcgt gccgcgcatg tggggcgggc tgaccacccc tggcgagctg cgcgccatcg
     8941 ccgacgccgc cgaaaagtac aaggtgccca ccgtcaaggt caccggcggc cagcgcatcg
     9001 acctgctggg cgtgaagaag gaagacctgc ccgccatgtg ggccgacttc gcccaggccg
     9061 gcatggtctc cggccacgcc tacggcaaga gcctgcgcac cgtaaagacc tgcgtcggcg
     9121 ccgagcactg ccgcttcggc acccagcgct ccatggacct gggcgtcaag ctcgaaaaga
     9181 tgctgttcgg catgtggagc ccccacaagg tcaagctggc ggtgtccggc tgcccgcgca
     9241 actgcgcgga gtcgggcatc aaggacgtgg gcgtgatcgg cgtcgattcc ggctacgagc
     9301 tctacatcgg cggcaacggc ggcatcaaga ccgaggtggc gcagttcttc tgcaaggtca
     9361 ccaacgacga cgaggtcatg gaatacggcg gtgccttcat ccagctctac cgggaagaag
     9421 gcttctatct ggaacggacc tgccactacc tggagcgggt cggcatggag cacatcaaga
     9481 aagaagtcct ggaagacgcc gacaagcgca aggccctcta cgacaagttg ctcttcgccc
     9541 tgcagaacta caaggacccc tggaccgaga tcgtcgagga ccgttccggc ggcgagttca
     9601 agcgccaatt cgaaatcatc aaggtataag ggtccgtcat gagcaactgg aaaaaaatct
     9661 gcgccgtcag tgacgtcccc gtcctgggct cccgcgtcgt gcatgcggaa gaggtcggca
     9721 tcatcgccgt cttccgcaac agcgccgacg agatcttcgc ggtgcgcgac aagtgccccc
     9781 acaagggcgg tcccctgtcc cagggcatcg tccacggcag caccgtcacc tgccccctgc
     9841 acagctggaa gatccagctg gaggacggcc aggtggtgcc gccggacgtg ggctgcgtga
     9901 agaagtttcc cgtcaaggtc gagaacggcg aggttttcct cggcctgtaa ggttcgcgct
     9961 ccgggggcga cgtcggctgc ggccgtctcc cccgtcgcct ccgcggcgtg agcctccccg
    10021 atttttaatg ttgaatcccc gtcggggtcc cttctggacc gccgtccggg ccctctcatc
    10081 cccaggagtc ccaaaatgga tcgtaaatcc ttcctccagg ccggtcacac ccccacgttg
    10141 atcgcggcct tcctctattt cgacctggcc ttcatggtct gggtgctgct cggccccctg
    10201 ggcgtgcaga tcgccaagga tctgggcctg tcccacgccg aaaagggcct gatggtcgcc
    10261 accccggtgc tcgcgggcgc cgccctgcgc atcgtcatgg gcgtgctggt ggatcacctc
    10321 aagcccaaga ccgccggcgc catcggccag gtgatcgtca tcggcgccct cgccgcggcc
    10381 tggaagctgg gcatccacaa ctacgaagaa accctgattc tgggcttctt cctgggcgtc
    10441 gccggcgcag cctttgccgt ggccctgccc ctggcttccc gctggtatcc gccggagcat
    10501 cagggcacgg ccctgggcat cgccggcgcc ggcaactccg gtactgccct ggccgccctc
    10561 ttcggccccg gtctggcggc agcctacggc tggaacaacg tcttcggcct ggcgctgatt
    10621 cccctgtcca tcgtcttcgt gctctacctc ttcatggcca aggatgcgcc ggaatgcccg
    10681 cccgccaagt ccctcaccga atacctcgcg gtgctcaagg acaaggacgc ctggtggttc
    10741 atgttcttct acagcgtcac cttcggtggc ttcgtcggcc tggcctcgtc cctgaccatc
    10801 tacttcaaca cccagtacgg tctggatccg aagaccgccg gcttctacac cgccgcctgc
    10861 gtcttcgccg gctccctggt gcgcccgatc ggcggcaacg tggctgaccg catcggcggc
    10921 atcaagagcc tgaccgtcat gtacgtgctg gccgccatct tcctggccat cgtcagcttc
    10981 ggtatgcccg aatcctggca ggccctgctg gtgttcgtct gcgccatgct gtccctgggc
    11041 atgggcaacg gcgccgtgtt ccagctcgtg ccccagcgct tccgcaagga aatcggcgtg
    11101 atgaccggcc tggtcggcat ggccggcggc gtggggggct tctacctggc ctccagcctg
    11161 ggctacagca aacaggtcac cggcagctat cagtccggct tcctgatctt cgccgccctg
    11221 gccatcgccg ccctggtcgg actcaccgcc gtgaagagcc gctggcgcac cacctggggc
    11281 gccccgcacc tcacctccgc aaagatctga gcacgacgac ggacactgcg accgcgcgga
    11341 tcacgccccg cagagttggc cctcccggcg ttctccgccg cccggtcctt gcaccggacc
    11401 acggggaacc gcccggtcgc ggcacacgtc cctccgcccg cccgccatgc tccgcctggc
    11461 gggcgggctt ttctgcatac tgagcgtctc accggaccgc ttccatgact gccgccacga
    11521 ccgccccgaa gaacgcccgc ctccaggccc gcatcggcca cgcctccttg accggccccc
    11581 gcccccgcaa cgaggacttc tgcggcgccg tcacccccga cggggccgag ctggacacca
    11641 agggcatcct ggccgtggtg gcggacggag tcggcggcca tgccaacggg cgcgaggcct
    11701 ccgagtacac ggtgcgcggt ttgctctcgg actactacgc cacccccgac acctgggccg
    11761 tcaacaaggc cctcgacacg gtgctcaacg ccctcaaccg ctggctcgtg tcccaggcct
    11821 cgcgcacccg tgaggctgcc ggcatggcca ccaccctgtc agccatcgtg gtgcgcggca
    11881 ggcgcttcta caccgcccac gtgggcgaca gccgcatcta ccgtctgcgc gacggccgct
    11941 tcgagcagct caccaccgac cacctgtggg aacacccgga actgaagaac gtcctgtccc
    12001 gcgccatcgg cctcgaccct cacctgtcgg tggactacgc cgacggcgac ctggagcccg
    12061 gcgacgtgtt cgccctgatg agcgacggcg tctggaacca gctcggcgac aaacgcatcg
    12121 ccgacatcct gcgcagccag gacgaacccc aggccgcggc ggcccgcctc gccggtgaag
    12181 ccgaggacgg cggcagccag gacaactgca ccgccctggt cgtgcgtgtg gacgccctgc
    12241 cccccgaccg cctgcgcgac accatcgccc gcctcggcca gctgacactg ccgccgcgcc
    12301 tcaagcccgg ccaggaactg gacggcctca ccgtggacgc cctgctccac gaatccatcg
    12361 ccaccctgct ctaccgcgtc cacgatgccc agggccatcc gcgggtcctc aagaccctgc
    12421 gccccgagcg caacgacccg gaagcctcgg ccgccctcgc ccacgaggag tggctggccc
    12481 gccgtgtgaa cgacgcctat tttccccagg tgattctcca cccggcacgg gcccacctgt
    12541 attacctgat gagctggcac gagggcgcca ccctcaaggc ccgactggag agcggacacc
    12601 gcttcgggct gggcgagatc gtcagcctgg gcgaacggct cctcaaaggt ctcgccggcc
    12661 tgcatcgcct gtccatcatc catcgggaca tcaagcccga caacctgcac ctggacaacg
    12721 acggccgcct gcgcattctc gacctgggcg tggccgcctc cgacagccgc ggcgacggcc
    12781 agcagttcca cgagatcaac aaccccggca cgcccagcta catggcgccg gaactctacg
    12841 gtcccacgcc gaccgccgcc aacgagcaga ccgacctgta cgcggtcggc gtcaccctgt
    12901 acgagctgct cacccgaaag tacccctacg gcgagatcga acccttccag aaccccaagt
    12961 tcggcgatcc cgtgcccccc acccgcttcc ggcctgacat ccccgcctgg atggaggcgg
    13021 tgctcctcaa ggcggtcgcc cgcgaccccc gtgcccgctt cgagaccgcc gaggaattcc
    13081 tcctcgccct ggaacggggc gcccaccgcc ccctcgtcgt gccgcgcaag tcgcccctgc
    13141 tccagcgcga cccgcaactg ggcctcaagc tcctcgccgc ggcctccctg accctcaaca
    13201 tcttgctcat ttatctgttg ctggtacgct agcgggcggt gtcccggtca cgagccggcc
    13261 cgacaccccc gcccgcgaac cgacatgatg cacacgcctg acccctcgca gcctcctccg
    13321 ccactgttcg accgcgagaa cccggggcaa ctgctgcgca ccatgggggg catctcgctg
    13381 ctcctgaccc tgatcgtcgg tgtgtcggtt ttcgcccagc acgatgtctc acccatccac
    13441 ggactgaccg ccctgggggt ctgcattgcc ctctgtgccg gcctgctcgg cttccgccgc
    13501 catcccgacc gcctgccgtc ccgttgcgtc ctgtggggcg cctggctgat catcctgatc
    13561 ggcgcagcct tcacgggcgg caaccgcacc acctcactct cggccatccc ggtcctcatc
    13621 gtcctcgtcg gctggctctt cggcatgcgc cacgccctgc tgatgacggc cgtgtccatc
    13681 ctcgccctgc tgggcatgtc cctctccgaa cagtacgact ggctgcccat gggcggatcc
    13741 tctccccccc tcagccgctg gatggtgctg agcaacgtgc tgctcctcac cgccttcctg
    13801 accctccact cgcgccggct ctacctgtca cgctaccagg ccctgcaccg cctgagtgcc
    13861 accctcggcc tgctcaccga gggcacaccc atcatgctcg ccgcggtcga cggccggggc
    13921 tgctacagct acgtcaatca gaattacgcc cacttccacg gcctggcccc caccgccctg
    13981 cgcggctccc tcgtggatgc ggtgctcggg gacggcatcc agcagcacaa tctcgcccgg
    14041 ctcgatgccg gcggcggcag cgccaccttc cgcagccgcc ggcacaaccg gcagaccggc
    14101 gacgagcgct ggatcgagac ctgtctgcgc agcgccaccg cgacggacgg acggaccgac
    14161 ggctattacg ccctgctgcg cgacatcacc gacgaggtac gcaccgaaga ggagatccgc
    14221 ttcctcgcct accacgacgc cctgaccgga ctgcccaacc ggatcctgct ggccgaccgc
    14281 ctgcgtcagg ccatcgcccg cgccgcgcgg gacgacaccc tggtggcggt gtgctacctg
    14341 gacctggacc gcttcaagtt cttcaacgac acctggggcc acaccgtggg tgacgagatc
    14401 ctcgtccagg tggcggggcg cctgcgcgac agcctgcgcg gttccgacac cgtggcgcgc
    14461 ctggggggcg acgagttcct ggtgctcatc ggcggcatcg ccgaccgcca ggagatgccg
    14521 gccgccctgg cccgtctgct gggaaccgtc agccagccgg tggtcctgga ggatggcgcc
    14581 gagatccgca tctccgccag cgccggcgtc gccctcttcc cccgcgacgg agaagaaccc
    14641 gacgtcctgc tgcgccacgc cgaccaggcc atgctcctcg ccaagcaaag cggccgcgcc
    14701 cgctacgccc tcttcgacac ggaactggaa cagcgcaatc accaccttca gcgcatcgtc
    14761 agcagcgtcg accgggggct cgacgggggc cagttccgcc tcttcttcca gcccaaggtc
    14821 aatctgcgcc tgagcagcgt catcggtgcc gaagccctga tccgctggga acaccccgac
    14881 gaaggcctgc tcctgccgga cgccttcctg ccctggctgg aaagcagccc ggtgggcgtg
    14941 cgcctggatc gctgggtcct cgccgcggcc ctcgaccaga tgagccggtg gcatgccgcc
    15001 gggctggaca tccccgtcag catcaacgtc tcgggccacc acctcctggc accggacttc
    15061 gtggaccacc tcaccgccca gctcatcgcc caccccagcg tcgcgccgga gcggctggag
    15121 ctggagattc tcgaaaccac ggccatggag gacgtggacc aggtggcccg cgtgatccgc
    15181 gcctgtacgg cgctgggcgt cagcttcgcc ctcgacgact tcggcaccgg ctattcctcc
    15241 cttacctatt tccgccgcct gccggcccgc accctcaaga tcgaccgctc cttcgtccgc
    15301 gacatcctcg ccgatcccga ggatctggcc atcgtcaagg gcgttgtcgc cctcgcccgc
    15361 agcttcggcc gacaggtgat cgccgaaggc gtcgaaacgc ccgagcactg cggcccgctg
    15421 ctgagcctgg gctgcgactg cgtccagggc tacggcatcg gccatcccat gccggcggcc
    15481 gaattcccgg gctgggtgcg cacctggaag tccgtgaacg gctgagcccc gcgcctgtca
    15541 cacccctgcc ggccacgtgt gccatcctgc cggcatcaac cctcaggaga accccatgca
    15601 ctacggcgaa ttcgacactg cgtcgctgat gttcatcacc cctcgcccgg aactgatctt
    15661 cgtgcgcggg gacgggtcct ggcttcatga cgacaccggc aagcgctatc tcgacttcgt
    15721 ccagggctgg gccgtcaact gcctcggcca ctgcccggcc gagatccgag aggcgctcgc
    15781 cgaccaggcc gcgcgcctga tcaaccccag cccggccttt ttcaacggcc cgatgaccga
    15841 actggccggc ctgctgaccg agcattcctg cttcgaccgg gtcttcttcg ccaacaccgg
    15901 cgccgaagcc aacgagggcg ccatcaagct cgcccgcaag tggggccgca tgcacaagga
    15961 cggcgcctac gagatcatca ccttcgagca cagctttcac ggccgcaccc tcgccaccat
    16021 gtccgcctcc ggcaagcccg gctgggacac cctgttcgcg ccccaggtgc caggttttcc
    16081 caaggcccgc ctgaacgacc tggactccgt cggggcgctc atcggcgaca agaccgtcgc
    16141 catcatgctc gaacccgtgc agggcgaagg cggcgtcatt cccgccgaac ccggcttcct
    16201 gcaagccctg cgggacctca cccgcgccca cggcctgctc ctcatcgtgg acgaggtgca
    16261 gaccggcatg ggccgtacgg gccgcctctt cgcccacgaa catgccggca tcgagccgga
    16321 catcatgacc ctcggcaagg gcatcggcgg cggcgtgccc ctgtcggcac tgctcgccaa
    16381 agaggccgtg tgttgtttcc agcccggcga ccagggcggc acctacaacg gcaatcccct
    16441 gatgaccgcc gtcggcgcgg ccgtgctgcg gcgcctgctc tccgacggct tcctggcccg
    16501 ggtcgacacc ctcggccgct acctggaaag tcgtctgcaa gcgctcgtca cccggcacgc
    16561 cctggccggc gaacggggcg caggcctgct gcgcgccctg atcctcgatt cggaccgcgg
    16621 cccggccctg gtcaaggcgg ccctggacta cgcgccggaa ggcctgctcc tgaatgcgcc
    16681 gcgccccaat ctgctgcgct tcatgcccgc cctcaccgtc accgaggccg agatcgatcg
    16741 catgatcgag atcctcgaca ccctcctcgg cgccgtgcgc gccgacgcgg gctgagcctc
    16801 gaaacacgtc ccgcgaccgg accaggtcgc ccatctggcc gccgtccccg gcgcaaggcc
    16861 cctcggcgcc ccatctcacg tcacgtcgcg cgcgggttgc gggttgcggg ttgcgggttg
    16921 cgggttgcgg gttgcgggtt gcgggttgcg ggttgcgcag tcgactctgc ccgcgccgcc
    16981 gaattccttg ggcccgaggc gacgcccgcc ttacaatagc gggctgtccc gcttcccctc
    17041 cggaagcccc tgaccacaaa ggatttccat gtctctcaag tgcggcatcg tgggtctgcc
    17101 caacgtcggc aagtccactc tcttcaacgc cctcaccaag gccggcatcg cggccgagaa
    17161 ctaccccttc tgcaccatcg agcccaacgt cggcatcgtc gaagtgcccg acccgcgcct
    17221 cgccgccctc tccgagatcg tcaagcccca gaaggtccag ccggccatcg tcgaattcgt
    17281 ggacatcgcc ggcctcgtcg ccggcgcctc caagggcgaa ggcctgggca accagttcct
    17341 tgccaacatc cgcgagaccg acgccatcgt caatgtggtg cgctgcttcg acgacgaaaa
    17401 cgtggtgcac gtgaacggca aggtcgatcc cctcgccgac atcgagacca tcgtcaccga
    17461 actcgccctc gccgatatgt ccgccgtcga aaaggccatc catcgggaaa acaagaaggc
    17521 ccgtgccggc gacaaggacg cccagaagct cgtcgccctg cttgaacggc tgatgcccca
    17581 cctcaacgaa ggccagccgg tacgcaccct gtccctgagc gatgacgaga agcaacttct
    17641 caagcccctg tgcctgatga ccctcaagcc cgccatgtac gtgggcaacg tgctcgaaga
    17701 cggctttgag aataacccct acctggagcg cctgcgcgaa cacgccgccg aggaaggcgc
    17761 gccggtcgtc gccctgtgcg ccaagatcga ggccgaactg gctgatctgg acgacgctga
    17821 caagctggcc ttcctggccg acctgggcct cgacgaaccc ggcctgaacc gcctcatccg
    17881 cgccggctac aagctcctcg gcctgcagac ctacttcacc gccggcgtca aggaagtccg
    17941 cgcctggacc atccacgtgg gcgacaccgc cccccaggcc gccggcgtca tccacaccga
    18001 cttcgaacgc ggcttcatcc gcgcccagac
//
LOCUS       DAOQMM010000074        17871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1174_length_17871_cov_52.769982, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000074 DAOQMM010000000
VERSION     DAOQMM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1174_length_17871_cov_52.769982"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLH85_12045"
     CDS             complement(<1..779)
                     /locus_tag="PLH85_12045"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB65342.1"
                     /translation="MNAIVKIENVGMSFDTKKGRFIALKDVNLQIRTGEVVSLIGHSG
                     CGKSTLLNLIAGLTLPSSGVMLCNGREIGGPGPERAVVFQNHSLLPWLSCFENVYLAV
                     ERVFGTKEGKAKLKARTEAALEMVHMGHASQKMPHEISGGMKQRIGIARAFAMEPKIL
                     LMDEPFGALDALTRASLQDELIGVMEKTGATVVMVTHDVDEAVLLSDRVVMMTNGPAA
                     TIGEILEVKLEKPRDRLALAHDARFAEYRAAILEFLYQKQM"
     gene            complement(800..1636)
                     /gene="ntrB"
                     /locus_tag="PLH85_12050"
     CDS             complement(800..1636)
                     /gene="ntrB"
                     /locus_tag="PLH85_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HNB65343.1"
                     /translation="MTAAPAAPATETPAVRSALGEKLGNFLRAVIPPLLGLALVVGLW
                     GLISMKASDFPSPAKTLEAAITLFSDPFYQNGPNDQGIGWNILSSLKRVGVGFGVAAL
                     VGIPMGFIIGRFGFMNAMLSPIISLLRPVSPLAWLPIGLMVFKAADPASTWTIFICSI
                     WPMILNTAQGVQRVPQDYLNVARVLNLSEMKIITKILFPSVLPYMLTGIRLSIGTAWL
                     VIVAAEMLTGGVGIGFWVWDEWNNLKVEHIIIAIFVIGVVGLLLENLLLLVARRFSYG
                     NA"
     gene            complement(1806..3062)
                     /locus_tag="PLH85_12055"
     CDS             complement(1806..3062)
                     /locus_tag="PLH85_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB65344.1"
                     /translation="MSKHNDDSILSRRGFLKAGASMGGLAAMGTMLPMGGVWAAGSDK
                     PELEEVKIGFIPLTDCSSVVMASVMGFDKKYGIKITPSKEASWAGVRDKLANGELHAA
                     HVLYGLIYGLHLGIGGPKKDMAVLMTLNNNGQAITLSNALRDKGAVDGESLAKVVAKK
                     EKDYTFAQTFPTGTHAMWLYYWLAANGIDPFKDVKNIVVPPPQMVANMRVGNMDGFCV
                     GEPWNQRAIVDKIGFTAITTQDIWPDHPEKVLGSTAEFAAKYPNTARAMICAILEASK
                     WIDASAGNRSKTAETVAAKSYVNTDVDVIRSRMIGQYENGLGKSWSEKNYMKFFNDGQ
                     VNFPWLSDGMWFLTQHRRWGLLKQDVDYLAVAKAVNKTELYKQAAGIVGVPVPKSDFR
                     SSKLVDGVVWDGKDPKKYAASFKIHA"
     gene            complement(3456..4031)
                     /locus_tag="PLH85_12060"
     CDS             complement(3456..4031)
                     /locus_tag="PLH85_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNB65345.1"
                     /translation="MLRVLVVDSSRERAAEVCAALAVAGHQVTAVLSSALELSEQVLS
                     SKPDVIFIQADSPSRDTLEHLAIVNRDCPRPVLMCSSDSDTELIRRAVKAGVSSYVVD
                     SVSPERVDALMDVALAQFEAFQNLRAELDDAQRKLSERIVVDRAKGVLMKARGLTEED
                     AYHALRKLAMERGRKLADVAQDVLDMARLLM"
     gene            complement(4146..5690)
                     /locus_tag="PLH85_12065"
     CDS             complement(4146..5690)
                     /locus_tag="PLH85_12065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB65346.1"
                     /translation="MADKKLRVLVADDIASNRSIVGAFLARLNCQPIFAEDGHDAVEL
                     FRQEAPDVVLMDLMMPRMDGFEAIRQIRSFGDDHWVPIIILSALSGEGDIVKGLEAGA
                     DDYLTKPLSFPVFAARFKALGRLLDMQRRLSGSLEQFRAVANGVIDGIITADEQGVIL
                     SVNRSASLIFGYRASEMIGRNLKMLMPSPFRDEHDAYLEHYVLTGERRMIGQIREVIG
                     LRKDGSTFPLELGVSDLTLPGKRMFIGVVRDISESQRIRRKLADDAARLQRYHDEQEQ
                     EQELARSIMERQIRSDWLHDASVQYSVTPASNFSGDVVTVARSPQGVLYAMLADATGH
                     GLAAAVSVLPVLGAFYRLVAQGAPLASIVQELNSLLCGFSFRGRFVAAVLVCVDERAR
                     SGAVWVGGVPDALLVDGQGAVVRRFSSAHLPLGITALGRDEVVPEVFSWAEDSQLILC
                     SDGLTEAENAAGEQFGVRRLEDALRASTPAGRVEGVRHALADHLRGHPAGDDMSVLIV
                     DCEGKA"
     gene            complement(5793..6698)
                     /locus_tag="PLH85_12070"
     CDS             complement(5793..6698)
                     /locus_tag="PLH85_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB65347.1"
                     /translation="MSEPRILVVDDEPFNLEIVGEYLDGMGFDLVYAESGTEAWEVLT
                     RAGEHFDLILLDRMMPGLDGISVLRQVKADPSLRQIPVIMQTAATSPEQVREGLAAGA
                     YYYLTKPFEGEALQTIIRSALDDQRIRRDLTTDLLNHAQALALVAEAEFEARTLDDAR
                     KLAAFVALLGPQPEVLAMGLSELLVNGVEHGNLGIDFAEKSRLREEDRWEEEIARRLA
                     LPENTDKRVRLRVRRDDGRWVFEIRDDGPGFDWRRFLDFEPERAFAPNGRGIALSRQL
                     SFASLTYLPPGNQVVATVPIQDGTP"
     gene            complement(6699..10085)
                     /locus_tag="PLH85_12075"
     CDS             complement(6699..10085)
                     /locus_tag="PLH85_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB65348.1"
                     /translation="MMRVAAGRRWLLPVSVFAVGALITTAFWHNLRVHEAAAEQARHD
                     AVVERLAQQVEERLGKVDFILRGAAGLFGAQKLVERGEFETYVDSLHLHDELPEVVGV
                     GLARRVETKRMPAFEAVLGGLYGRPVRVYPQPDPAARALSFPITFFWPTGIVAARVVG
                     FDGYADPIRRRAMDLALQGERLAMTAAVKLAAFDQGESANAVTLYRLARAPEDASVVS
                     EAPGLVVLGLNLNVLFNHLFGAMGKAYGLEVEDVTDAAPLRLFYAPLAEADPAAPQAS
                     RQVSYGDRIWRLSVTPLAAGEQAGASGALALSGLATSLLAAFLMASVMGRRERAEDLA
                     LRMTDELRSSERRFELAVSATEEGIWEWRSDQRTLYLSSRCDRLLGYREGTLPRSARG
                     VLRNFTRASRRDLLLALRRHTKAPEVPLYCVLQVRRIDGSVGWFHVAGKAEFDAAGRH
                     VRTAGAASDVTELRRAQQAVTESHAKLDALYRNAYFGMAMVDGESRYLQVNPEFCRIV
                     GYSEEELREMSFYDLTPKEFLARDTRALVDLSLGVRSPPYEKEYIRKDGSRVPVVIAT
                     TYVGGSDKARWVVVEDATARKASQQAIQEAHATNESLISAMPDMLVQLDGQMRIVRYH
                     ADDRHSLSMPAESLLGKRVDEALPPEVAERFGRAALRASRSGEVQRVEYSTREGGASR
                     HFEARFRAVRTGGTLAVVRDVTDLKEAELALRESEARWQFALDGAGDGVWDWNLSTGK
                     VFRSTRWVSMLGYAPEDLDGSIDAWSSRVHPDDLPATLEAQAAHLRGETPIFTREFRL
                     RCKDGAYKWILARGLVVERDAAGRPLRMIGTQTDIDQVKAREAEILYHNTNLASLVEA
                     RTKDLMAAKEAAEAANEAKSIFLANMSHEIRTPMHGVLSYASLGESRIGAVSDDKLRS
                     YFNRIRLSGERLLLLLNDLLDLSKFEAGRMILNFEPTDMDTLVREVVREFDALLAARH
                     LSVKVQRDGVEPALFDGDAARVSQVIRNLLSNAAKFTPEGRAIHVHIRHDSLPEGRRA
                     SDLRRLPAVAVAVSDEGVGIPPEELDAVFEKFVQSSRTRTGAGGTGLGLAICREIVEA
                     HRGAISVCNNAAGGAEFTVILPVSRAAPRSAPSDKEREES"
     gene            complement(10352..11173)
                     /locus_tag="PLH85_12080"
     CDS             complement(10352..11173)
                     /locus_tag="PLH85_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65349.1"
                     /translation="MTDRVWLIGDDAGVAERLDAWISDAGSPCERLTSREAVSRLTDG
                     GGPIKAAVVDAGDGHAVLALLEALGRRCKTTVLLVDAECYREIAAALAAGIGTFLRKP
                     VEPAALAAALTVEGRAERPGSGRFLFRTLDDVLDLSQLVAGLCPDPAAVEIGLSELML
                     NAVEHGNLGIGFERKGHLMNAGTWRQELERLLRLPENLVRFGYLDVELRGDGVRFKVL
                     DQGSGFDPQPYMNFNPERSVELHGRGIAIARMLAFPDLHFREEGSCVEGLVRRVC"
     gene            complement(11263..11631)
                     /locus_tag="PLH85_12085"
     CDS             complement(11263..11631)
                     /locus_tag="PLH85_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNB65350.1"
                     /translation="MAVAIVLFAHGARDAEWAEPARRVADAVQARAPAARVSLAFLEF
                     MAPTLAEAVEAAAADGATEVRVVPLFLARGGHLKRDVPALVEAARARHPGCRIELLPP
                     VGEDRGVVDAMAAYAIEAGH"
     gene            11871..12656
                     /locus_tag="PLH85_12090"
     CDS             11871..12656
                     /locus_tag="PLH85_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB65351.1"
                     /translation="MSLPAHAGLHPEAVPGGVVELPVAARGNARPEVRYDGHPVLLQR
                     RGEQWIAVVGIPLDAPVGEQHLDVGPADAPQAQPFPVHAKAYPVQKLTLKNPKMVNPD
                     EADLARIAAERDRQIEIRSRFRDVPVLRTDLDLPAVGRLSSRFGLRRVFNGEARSPHT
                     GLDVAVPAGTPVKAPADGVVSHVGDFYFNGRTVFVDHGQGFVSMVCHLTSASVEAGQA
                     VRRGEVLGLSGSSGRATGPHLHWSVYLNGAAVDPALFIPPPAR"
     gene            complement(12634..13431)
                     /locus_tag="PLH85_12095"
     CDS             complement(12634..13431)
                     /locus_tag="PLH85_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNB65352.1"
                     /translation="MTDAFSGGAEDPRRFGGIARLYGEAGRARLAAARVAVVGIGGVG
                     SWAAEALARSGVGALTLIDLDHVAESNLNRQIHATEATLGQAKVDAMAARIAGYAPGC
                     AVTRVDDFVTVENAATLLGAGFDYVVDAIDAVRVKVAMIAVCRAAGVPVVTCGAAGGQ
                     IDPTQIRVDDLARTIQDPLLAKVRGQLRKAHGFPRDGKKKFGVEAVFSTEPLRYPAPA
                     TVCDAAGGGAPQGPAGLNCAGFGSVVTVTASVGLFAAARALNGLAAG"
     gene            complement(13433..14386)
                     /locus_tag="PLH85_12100"
     CDS             complement(13433..14386)
                     /locus_tag="PLH85_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB65353.1"
                     /translation="MQGLTSYGHGIYAVDSGYTGTGIAAIHLIEEGGRAALVDTGNNA
                     ALVHVEEALKATGLKPEAVDYVLLTHIHLDHAGGAGAFMKAFPNAKLVVHPRGARHMA
                     EPSKLFAGVSAVYGPERAKELYGELIPVPVARIVEATDGLELSLAGRVIRVLDTPGHA
                     RHHVCYFDTGSRSFFTGDTFGVSYRHLDVDGRASIFPTTSPVQFDPVAMHASVARMEA
                     EQPVAMYLTHYAQLTEVPRLAADLHRLIDTHVAIAERHAGLPGGEPRQQAIEADLWAM
                     VADEARNAGWRLPEADWRRLLAVDVELNAQGLDVWLDSRSA"
     gene            14526..14789
                     /locus_tag="PLH85_12105"
     CDS             14526..14789
                     /locus_tag="PLH85_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65354.1"
                     /translation="MLTIQDCIELSDLTEDEVLAIAEHEHLPEMLAVEMGCYLCHTPD
                     GDKRIRRMIADDIRHAHALGDVQRTALLKSVLKHYVQEHHGNA"
     gene            14860..15888
                     /locus_tag="PLH85_12110"
     CDS             14860..15888
                     /locus_tag="PLH85_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65355.1"
                     /translation="MAILRCNHCGHVAETLVDANGQNMPCPRCITPAPVYDTVVFIRK
                     VLDQYFQLRDELNRLKAGASAMMQAEPARLATATATTAAPCDPPPVARPPAGARPNQF
                     ALNSQFAAIEQWFLRRQIQIRPPTDAPELSPAFERTAADLGRYHTLLRDFFERIPATP
                     TPDDNRLHLDLNARNAQEIGDLDAVFARLHGSAFLSHYTLDKPDRSLHAALPADPAIL
                     RFLSGEWLEWYSLGRLAEHFRKKGLRAAGARGLTALLADGDERPLDVFFLVNGNTPIC
                     LACRTGDVRPDIDTLLRLRKRLGIPRGQFIVCSPTLAEEQAEHLSTAHELSCVTPAGL
                     ITHIERLF"
     gene            15976..16980
                     /locus_tag="PLH85_12115"
     CDS             15976..16980
                     /locus_tag="PLH85_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65356.1"
                     /translation="MALLRCNQCGHVAEPAADTVGQHLPCPHCGTSVPVYDTVLFVRK
                     VLQQYFALRDENTRLKDALAAAPATTEEAPAAPASAPPLSDIDLHNSSHFASEAQHAA
                     IQQWFARRQIEARPNRDAVDTTGFYDEIAVALGDHYELYREVLDKIRWGHKKDIPQFS
                     LNLTERSQKDGQALNAFCRQLHDSAFLSKYFYQKQDKIIRASVQTAPAIRAFFAGEWL
                     EWYALMKLLAFFRDRGLGFSCTRNLHITFANEDLHELDVFFLLDDGTPVCVECKTGEF
                     RADIDKMLRLRKRLGIDRSQFIVCALGLSDEQAAGLSGTYELSFVTPAGLLPHLARLF
                     "
     gene            17015..>17871
                     /locus_tag="PLH85_12120"
     CDS             17015..>17871
                     /locus_tag="PLH85_12120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNB65357.1"
                     /translation="MTPRARELLSHVFGYSGFRGPQEAIVEHVAAGGDALVLMPTGGG
                     KSLCYQIPALLRPGVAIVVSPLIALMQDQVSALKEVGVAADFLNSSLAFEQAMEIERA
                     LLAGALDLLYVAPERLVTPRFLDLLDHLREAGQLALFAIDEAHCVSQWGHDFRPEYLQ
                     LSLLPERYPHIPRIALTATADAQTRREIAERLCLTDAREFVSSFDRPNIRYTIVPKDN
                     PRAQLLAFIRDGHQGEAGIVYCSSRKKVDETAAWLLEKGIAALPYHAGLPAEQRATHQ
                     DRFLREDGIV"
BASE COUNT         3054 a         6647 c         5387 g         2783 t
ORIGIN      
        1 ttcatctgct tctggtacag gaactcgagg atcgccgccc gatattccgc aaagcgcgca
       61 tcgtgggcca gggcgaggcg gtcacgcggt ttctcgagct tcacttcgag gatctcgccg
      121 atggtcgcgg ccgggccgtt ggtcatcatc accacccggt cggacaggag cacggcctcg
      181 tccacatcgt gggtcaccat caccaccgtg gcgccggtct tttccatcac gccgatcagc
      241 tcgtcctgca gggacgcgcg ggtcagcgca tccagcgcac cgaagggttc gtccatcagc
      301 aggatcttgg gctccatcgc aaaggcccgg gcgatgccga tgcgctgctt catgccgccg
      361 gagatctcgt ggggcatctt ctgggacgcg tgccccatgt gcaccatctc cagcgccgct
      421 tcggtgcgcg ccttcagctt ggccttgcct tccttggtac cgaacacgcg ctcgacggcc
      481 aggtagacgt tctcgaagca gctcagccag ggcagcaggg agtggttctg gaagaccacc
      541 gcccgctccg gaccgggccc gccgatctcg cggccgttgc acaacatcac gcccgaactg
      601 ggcagggtga ggccggcgat gaggttgagc agcgtggatt tgccgcagcc ggagtggccg
      661 atcagggaca cgacctcacc ggtacggatc tgcagattga cgtccttgag ggcgatgaaa
      721 cggcccttct tggtgtcgaa gctcatgccg acgttttcga tcttgacgat agcgttcatg
      781 ttgaggctcc tcggggcaat caggcgttgc cgtagctgaa gcggcgggcc accagcagca
      841 acaggttttc cagcagcaga cccaccacgc cgatcacgaa gatcgcgatg atgatgtgct
      901 cgaccttcag gttgttccat tcgtcccaca cccagaagcc gatgccgacg ccgccggtga
      961 gcatttctgc agccacgatc accagccacg cggtgccgat ggacaggcgg atgccggtga
     1021 gcatgtaggg cagcaccgag gggaacagga tcttggtgat gatcttcatc tcggagaggt
     1081 tcagcacccg cgccacgttg aggtaatcct ggggcacgcg ctgcacgccc tgggcggtgt
     1141 tgaggatcat cggccagatg gagcagatga agatggtcca ggtggaggcc gggtccgccg
     1201 ccttgaagac catcaggccg atgggcagcc aggccagcgg cgataccggg cggagcaggc
     1261 tgatgatcgg cgacagcatg gcgttcatga agccgaagcg gccgatgatg aagcccatgg
     1321 ggatgcccac cagcgccgcc acgccaaagc ccacgccgac gcgtttgagg gaagacagga
     1381 tgttccagcc aatcccctga tcgttggggc cgttctggta gaaggggtcg gagaacagcg
     1441 tgatggccgc ttcgagggtc ttggccgggc tggggaagtc ggaggccttc atggaaatca
     1501 ggccccacag gccgaccacc agggccagac ccagcagggg cggaatcacg gcccgcagga
     1561 agttgccgag tttttcaccc agggcactgc ggacggcggg ggtttcggtg gcaggggcgg
     1621 caggcgcagc cgtcatggaa gtctcctggg aggtcgcggt ttcactgcgc accggcacaa
     1681 ggggcgcagg cgcggcaggg gacagggcca cggtcggatc gagggtcgcg gtcgtcatcg
     1741 tgaaatctcc ggttcaggtt gaatctgtga aaggtacgga cccatcgggt ccgtaccccg
     1801 tgtcatcagg cgtggatctt gaagctggcg gcgtacttct tcgggtcctt gccgtcccac
     1861 accacgccat ccaccagctt ggagctgcgg aagtcggact tgggtaccgg cacgccgacg
     1921 atgccggccg cctgcttgta gagctcggtc ttgttgaccg ccttggccac ggccaggtaa
     1981 tccacgtcct gcttgagcag gccccagcgg cgatgctggg tgaggaacca catgccgtcg
     2041 gacagccacg ggaagttcac ctgcccatcg ttgaagaact tcatgtagtt cttttcggac
     2101 cagctcttgc ccaggccgtt ttcgtactgg ccgatcatgc ggctgcggat cacatccacg
     2161 tccgtattca cgtagctctt ggccgccacg gtttccgcgg tcttggagcg gttgccggcg
     2221 ctggcgtcga tccacttgga ggcttccagg atcgcgcaga tcatggcgcg ggcggtgttc
     2281 gggtacttgg cggcaaactc cgccgtcgag cccagcacct tttcgggatg gtcgggccag
     2341 atgtcctggg tggtgatggc ggtgaagccg atcttgtcca cgatggcgcg ctggttccac
     2401 ggctcgccca cgcagaaccc gtccatgttg ccaacgcgca tgttggccac catctgcggg
     2461 ggcggcacga cgatgttctt cacatccttg aacggatcga taccgttggc cgccagccag
     2521 tagtagagcc acatggcgtg ggtgccggtg gggaaggtct gggcgaaggt gtaatccttc
     2581 tccttcttcg ccacgacctt ggcgagggat tcgccatcca ccgcgccctt gtcgcgcagc
     2641 gcgttggaca gggtgatcgc ctgaccattg ttgttgaggg tcatcagcac ggccatgtcc
     2701 ttcttgggac cgccgatgcc caggtgcagg ccatagatca ggccgtacag cacgtgggcc
     2761 gcgtgcagct cgccattggc gagcttgtcg cgcacaccgg cccaggatgc ttccttgctc
     2821 ggcgtgatct tgatgccgta tttcttgtca aagcccatca ccgaggccat gaccacggaa
     2881 gaacagtcgg tgagcgggat gaacccgatc ttcacttctt ccagttccgg cttgtccgag
     2941 ccggctgccc aaacgccgcc catcggaagc atcgtaccca ttgctgccag tcctcccatg
     3001 cttgccccgg ccttgagaaa gccgcggcgg gaaagaatcg aatcgtcgtt gtgcttgctc
     3061 atagctcgcc cctgaatgca aaaaaaacgg gcgtcacatc gccccgcgga tggcatgcgg
     3121 gggatggacg cccttgtcca aagtctgatc gcctgatcaa gccggaccgc cgttggaccg
     3181 acttcattca agacttagcg agaggcgtgc cagaaagatc aagtcattga tttacatcaa
     3241 aataacaaaa aagcgaaaat ttcaaacccg acctacccct aaagtggtag ccggaaagtg
     3301 ggtcaacagc gcactgctgc ggtgcaaaac gcctggcttt cgcactgttc cggaccatgc
     3361 acgcccattc accggcgtct caaaacagac agggcagggt cgcctgcacg cgccctgccc
     3421 tgctcccagc ctgtccgatg cccctgccgg agtggtcaca tcagcaggcg ggccatgtcg
     3481 agtacgtcct gggccacgtc cgcgagcttg cgcccgcgct ccatcgcgag cttgcgcagg
     3541 gcatgatagg cgtcctcttc ggtgagcccg cgcgccttca tcagcacccc cttggcacgg
     3601 tccaccacga tgcgttccga cagtttgcgc tgggcgtcat ccagctcggc gcgcaggttc
     3661 tggaaggcct cgaactgggc cagcgccacg tccatcaacg catccacccg ctccgggctc
     3721 acagaatcaa cgacatagga cgacacgccg gccttcaccg cgcgccggat cagttcggta
     3781 tccgaatccg aagagcacat cagcaccggc cgcggacagt cacgattgac gatcgccaga
     3841 tgttcaaggg tgtcgcggct cggggaatcg gcctggatga agatgacgtc cggcttcgag
     3901 ctcaacacct gctccgaaag ctccagggcg ctcgacagga ccgccgtcac ctgatggcca
     3961 gccaccgcca gggccgcaca gacttcggcg gcccgctcac gggaagaatc gacgacgagg
     4021 acacgcagca tgggatggtc cggacaacgc aatcagcagc cagaattcag caggaaccgt
     4081 gccaaatcag gcaccggcgc aaggatcgga cgatcagccg ccggtgtcca ggtccgctcc
     4141 cgtgctcagg cctttccctc gcaatcgacg atcagcaccg acatgtcgtc gccggccgga
     4201 tggccacgca gatgatctgc aagggcatga cggactccct cgacccgccc ggcgggcgtc
     4261 gacgcccgca aggcgtcctc gaggcgacgg acgccgaact gctcaccggc ggcattctcc
     4321 gcttcggtga gaccatccga acacaggatg agctggctgt cctccgccca gctgaagacc
     4381 tccggcacca cctcgtcccg ccccagggca gtgatcccca gggggagatg agccgacgag
     4441 aatcgccgga ccacggcgcc ctgaccatcc accagcaggg catcgggcac gccaccgacc
     4501 cacaccgcgc ccgagcgggc gcgctcatcc acgcacacca gcaccgcagc gacaaagcgc
     4561 ccacggaagg agaagccaca cagcaggctg ttcagttcct gcacgatgga cgccagcggc
     4621 gcgccctggg ccaccagccg gtaaaaggcg cccagcaccg gcagcacgct caccgcggcc
     4681 gccaggccat ggccggtggc atcggccagc atggcataca ggacgccctg cggcgaacgg
     4741 gccaccgtca ccacatcgcc ggagaagttg ctcgccggcg tcaccgagta ctggaccgac
     4801 gcatcgtgca accagtcgct gcggatctgc cgctccatga tgctccgggc gagctcctgc
     4861 tcctgttcct gctcgtcgtg gtagcgctgc aggcgggccg cgtcgtcggc cagcttccgg
     4921 cggatgcgct gagactcgga gatgtcccgc acgaccccga tgaacatgcg cttgcccggc
     4981 agggtcaggt ccgacacccc cagctccagg ggaaacgtcg agccgtcctt gcgcagaccg
     5041 atcacctcgc ggatctgtcc gatcatgcgg cgctctccgg tgaggacgta gtgctccagg
     5101 taagcgtcgt gctcgtcgcg gaagggcgag ggcatcagca tcttgaggtt gcggccgatc
     5161 atctcgctgg cgcggtagcc gaagatcagg ctcgccgagc ggttcaccga caggatgacc
     5221 ccctgctcgt cggccgtgat gatgccgtcg atcacgccat tggccaccgc ccgaaactgt
     5281 tccagcgaac cggacaggcg ccgctgcatg tccagcaggc gacccagggc cttgaagcgg
     5341 gccgcgaaga cggggaagga cagcggcttg gtcaggtagt catcggcgcc cgcctccagc
     5401 cccttgacga tgtctccctc gccgctcagg gccgacagga tgatgatggg cacccagtgg
     5461 tcgtccccga aggagcggat ctggcggatc gcctcgaagc cgtccatgcg cggcatcatc
     5521 aggtccatca gcaccacgtc cggggcctcc tgacggaaga gctcgaccgc gtcgtgaccg
     5581 tcctctgcaa agatcggctg gcagttcagc cgtgccagga aggcgcccac gatggagcgg
     5641 ttcgaggcaa tgtcgtcggc aaccagcacg cgaagcttct tatcggccat ggcgtacatt
     5701 tccctgttga gtcggattca aggcgagagc gggatccccg ccggcgcaca cgacgctgcc
     5761 tggcccgccg gaggcatggg acacgcggcc gctcacggcg ttccatcctg aatcggaacg
     5821 gtggccacca cctgattgcc cggcggcaga taggtgaggc tcgcgaacga cagctgacgc
     5881 gacagcgcga tgccgcgccc attcggcgca aaggcccgct ccggctcgaa atccaggaag
     5941 cgccgccagt cgaatcccgg cccgtcgtca cggatctcga agacccagcg cccgtcgtcc
     6001 cggcgcaccc gcaggcgcac gcgcttgtcg gtattttccg gcagcgccag acggcgggcg
     6061 atctcctctt cccagcggtc ctcttcccgc aggcggctct tttcggcgaa gtcgatgccc
     6121 agattgccat gctccacgcc attgacaagc agctccgaca ggcccatcgc cagcacttcc
     6181 ggctggggcc cgagaagcgc cacgaaggcc gccagcttgc gcgcatcgtc cagggtgcgc
     6241 gcctcgaatt cggcctccgc caccagcgcc agggcctggg cgtgattgag caggtcggtg
     6301 gtcagatccc ggcggatgcg ctgatcgtcg agggccgagc ggatgatggt ctgcagggcc
     6361 tcgccttcaa aaggcttcgt gaggtaatag taggcccccg ccgccagccc ctcgcgcacc
     6421 tgttccggtg acgtggccgc cgtctgcatg atcaccggga tctgccgcaa gctcgggtcc
     6481 gccttgacct ggcgcagcac cgagatgccg tccagtccgg gcatcatgcg atccagcagg
     6541 atcaggtcga agtgctcgcc cgcacgggtc agcacctccc aggcttcggt gccggactcg
     6601 gcatagacca gatcgaagcc catgccgtcc aggtactccc cgacgatctc cagattgaag
     6661 ggctcgtcgt ccacgacgag aatcctcggc tcgctcattc aactctcctc tcgttccttg
     6721 tccgacgggg cgctgcgcgg ggccgcgcgg ctgaccggca ggatcaccgt gaattcggcc
     6781 ccgccggccg cgttgttgca cacggagatc gcgccgcgat gggcctccac gatctcccgg
     6841 cagatggcca gcccgagccc ggtcccaccg gcgccggtgc gggtacggct gctctgcacg
     6901 aatttttcaa agacggcgtc cagttcctcg ggcggaatgc ccacgccctc gtcggagacc
     6961 gccaccgcca ccgcgggcag gcgccgcaag tccgacgcac gtcgcccttc gggcaggctg
     7021 tcgtggcgga tgtgaacgtg gatggcgcgc ccttccggtg tgaacttggc cgcgttggac
     7081 agcaggttgc ggatcacctg ggagacccgc gccgcgtcgc cgtcgaaaag ggccggctcg
     7141 accccgtccc gctgaacctt cacgctcagg tgccgggccg ccagcagggc gtcgaactcg
     7201 cgcacgactt cccggaccag ggtgtccatg tccgtcggtt caaaattgag gatcatccgg
     7261 ccggcttcga acttggacag atcgagcagg tcgttgagca gcagcagcag acgctcgccg
     7321 gacaggcgga tgcggttgaa gtaggaacgc agcttgtcgt cgctcaccgc accgatgcgc
     7381 gactctccga gactggcgta gctcagcacc ccgtgcatgg gggtgcggat ctcgtgggac
     7441 atgttggcca ggaagatgga cttggcctcg ttggccgcct ccgcggcttc cttcgcggcc
     7501 atcaggtcct tggtccgcgc ctccaccaga ctggccagat tggtgttgtg gtacaggatc
     7561 tccgcctccc gcgccttcac ctggtcgata tccgtctggg tgccgatcat gcgcagcggc
     7621 cggcccgccg cgtcccgctc gacgaccagt ccccgggcga ggatccattt gtacgcccca
     7681 tccttgcagc gcaagcggaa ttcccgtgtg aagatcggtg tctcgccgcg caggtgcgcc
     7741 gcctgggcct ccagcgtggc cggcaggtcg tccggatgga cgcggctcga ccaggcgtcg
     7801 atggacccat ccagatcctc gggcgcgtag cccagcatgc tcacccagcg ggtcgaacgg
     7861 aacaccttgc cagtggacag gttccagtcc cacaccccgt cgccggcacc atccagggca
     7921 aactgccagc gcgcctcgct ttcccgcagg gccagctcgg cttccttcag atcggtcaca
     7981 tcccgcacca ccgccagcgt accgccggtc cgtaccgccc ggaaacgcgc ctcgaagtgg
     8041 cggctggcgc ccccttcgcg ggtggaatac tccacgcgct gaacctcgcc gctgcgcgac
     8101 gcacgcagtg cggccctgcc gaaacgctcc gccacctccg gcggcagagc ctcatccacc
     8161 cgtttgccga gcagggactc tgccggcatg ctcaggctgt gacggtcgtc cgcgtgatag
     8221 cgcacgatgc gcatctggcc gtccagttgc accagcatgt cgggcatggc cgagatcagc
     8281 gactcgttgg tggcgtgcgc ctcctggatc gcctgctggc tggccttgcg ggcggtcgca
     8341 tcctccacca ccacccagcg cgccttgtcg ctgccgccca cgtaagtggt ggcgatgacc
     8401 accggcacgc gggaaccgtc cttgcggatg tattccttct cgtagggcgg cgagcggacg
     8461 cccagggaca ggtccaccag cgcgcgggtg tcccgcgcca ggaactcctt cggcgtcagg
     8521 tcatagaagc tcatctcccg cagctcttcc tcggaatacc cgacgatgcg gcagaactcc
     8581 ggattcacct ggagataccg ggactccccg tccaccatgg ccatgccgaa ataggcattg
     8641 cggtacaggg catccagctt ggcgtgggat tccgtgacgg cctgctgggc gcggcgcagt
     8701 tcggtgacat cggacgcagc cccggccgtg cgcacatgcc gccccgcggc gtcgaactcg
     8761 gccttgccgg ccacgtggaa ccagcccacg ctgccgtcga tgcgccgcac ctgcagcaca
     8821 cagtagaggg gcacctccgg cgccttggtg tggcggcgca gggccagcag caggtcgcgg
     8881 cgcgacgccc gcgtgaagtt gcgcaacacc ccgcgcgccg agcgcggcaa ggtgccctcg
     8941 cgatacccca gcaggcggtc gcaacgagac gacagataca gcgtgcgctg atccgaacgc
     9001 cattcccaga tgccttcctc ggtggccgac accgccagct cgaaccgccg ttcgctactg
     9061 cgcagctcat cggtcatgcg cagcgcaaga tcctcggcgc gctcacgccg ccccatcacg
     9121 ctggccatca ggaaagccgc cagcagactg gtcgccaacc ccgacagagc cagcgcaccg
     9181 gacgcgccgg cctgctcacc ggccgccagc ggcgtgacgc tcaggcgcca gatccggtcg
     9241 ccgtagctca cctgacggct cgcctgcggc gcagccggat ccgcctccgc gagcggcgcg
     9301 tagaacaagc gcaggggcgc cgcatcggtg acgtcctcca cctccaggcc gtacgccttc
     9361 cccatcgccc cgaagagatg attgaacagg acgttcagat tgagcccaag caccaccagc
     9421 cccggcgcct cggacaccac gctggcatct tccggagccc gcgccaggcg gtacagggtg
     9481 accgcattgg ccgactcgcc ctgatcgaat gccgccagct tcacggcggc cgtcatggcc
     9541 agtcgctcgc cctgcagcgc gagatccatc gcgcgacggc ggatcgggtc cgcgtagccg
     9601 tcaaagccca ccacgcgcgc ggcgacgatt cccgtgggcc agaagaaggt gatcggaaag
     9661 ctcagcgcgc gggcagcggg gtctggctgc ggatagaccc gcacgggccg cccgtagagt
     9721 cccccgagga cggcctcgaa ggccggcatg cgcttcgtct ccacccgccg ggcgagcccg
     9781 acccccacca cctcgggcag ctcgtcatgc aggtgcaggc tatccacgta ggtttcgaac
     9841 tcgccccgct ccaccagctt ctgggcgccg aacagccctg cggccccgcg caggatgaaa
     9901 tccaccttgc cgaggcgctc ctccacctgc tgggccagcc gctccaccac cgcgtcatgg
     9961 cgggcctgct cggccgccgc ctcatggacg cgcaggttgt gccagaacgc cgtggtgatg
    10021 agcgccccca ccgcaaacac cgacaccggc agcagccatc gtctgccggc ggcgacacgc
    10081 atcatcggga agggaggagg aatccggggc ataacgccct gtgaacggcg tgtcgcctca
    10141 aaccttgagc cgcatcgccc cccttccggc cggcggttca ggcgccggcg cgtggggtcg
    10201 gccgtctcgg ccaaggcagc gcattgtcat cggattggca cacgcgggcg tcacgcaaag
    10261 ccgctgcggg gcggcacccc ggtctggccg gtcaatctgc cggattcccg ctcagggcgc
    10321 ccggagcccg cgtccgcctg cagcgccgga ctcagcagac gcgacgcacc agtccttcga
    10381 cgcaggaccc ttcctcgcgg aaatgcaggt ccgggaaggc cagcatgcgc gcgatggcga
    10441 tgccacgtcc gtgcaattcc accgagcgct ccggattgaa gttcatgtac ggctgcggat
    10501 cgaagccgct gccctggtcg agcaccttga agcgcacgcc gtcgccccgc agttccacgt
    10561 ccagatagcc gaagcggacc agattctccg gcaggcgcag gagtcgctcc agctcctgcc
    10621 gccaggtgcc ggcgttcatg aggtgcccct tgcgctcgaa gccgatgccc agattgccgt
    10681 gttccacggc gttgagcatc agctcggaga gcccgatctc caccgccgcc ggatcggggc
    10741 acagaccggc caccagctgc gacaggtcga gcacatcgtc cagcgtgcgg aacaggaagc
    10801 gtccgctgcc cggccgctcg gcgcgcccct cgaccgtcaa tgccgccgcc agcgccgccg
    10861 gctccaccgg cttgcgcagg aaggtcccga tcccggcggc cagcgccgcg gcgatctccc
    10921 ggtaacattc cgcatccacc agcagcaccg tcgtcttgca gcgacgcccg agcgcctcga
    10981 gcagcgccag aacggcatgc ccgtctcccg cgtccaccac ggcggccttg atcgggccgc
    11041 cgccatcggt cagccgcgag acggcttcgc gggacgtcag gcgttcgcat ggactgcccg
    11101 cgtcgctgat ccaggcgtcc agcctctctg ccacgcccgc atcgtcacca atcagccaca
    11161 ctcggtccgt catcgaatca agctcctcca ccggtcgcct ttgaccggcc tctttccatc
    11221 cggtccgtcc cgccttcggg cggtcggacc gtgctcacat cctcagtgtc cggcctcgat
    11281 ggcgtacgcc gccatggcat ccaccacccc ccggtcctca cccaccggcg gcaacagctc
    11341 gatccggcag cccgggtgac gcgcccgtgc cgcctccacc agcgccggca catcccgctt
    11401 cagatgccca ccgcgggcca ggaacagggg caccacgcgc acttccgtgg ccccgtctgc
    11461 tgccgccgcc tcgaccgcct cggccagggt cggcgccatg aactccagaa aggccagact
    11521 cacccgtgca gcaggcgccc gcgcctgcac cgcatcggcc acccggcgtg ccggttcggc
    11581 ccattccgca tcccgcgccc cgtgggcaaa cagcacgatc gccaccgcca ttcaacactc
    11641 cgtattccct tggcccgcca gccatgcccg agccgtgcaa gaatcggctc cgcaaggctc
    11701 cgcgccccat tcggcgcgtg cgcatgccaa gaataatgtt aatccctgaa aatcaagcca
    11761 ggccccattt tgcccacgcg gcataagaac cttcagccat gtgtgaaata ttgcccattt
    11821 atcggcaact tgtccttcgt ctgcttgtcg gcgtcaccgc cggcgcgggc gtgagtctgc
    11881 ccgcccacgc cggactgcat cccgaagccg tccccggcgg cgttgtcgaa ctccccgtcg
    11941 ccgcccgcgg caacgcccgc cccgaggtcc gctacgacgg tcacccggtc ctcctccagc
    12001 gccgtggcga acagtggatc gccgtggtgg gcatccccct cgacgcgccc gtcggcgaac
    12061 agcatctgga cgtaggcccc gccgacgcac ctcaggcgca gcccttcccg gtgcatgcca
    12121 aagcctaccc ggtgcagaag ctcaccctca agaaccccaa aatggtcaat ccggacgaag
    12181 ccgaccttgc ccgcatcgcc gccgaacggg accgccagat cgagatccgc agccgcttcc
    12241 gcgacgtccc cgtgctgcgc accgacctgg acctgcccgc cgtcggacgc ctctccagcc
    12301 gcttcgggct gcgccgtgtc ttcaatggcg aagcccgctc gccccacacc gggctggacg
    12361 tggcggtgcc cgccggcacg ccggtcaagg cgccggccga cggcgtggtg agccatgtgg
    12421 gggatttcta cttcaacggc cgcacggtct tcgtcgatca cggccagggc ttcgtcagca
    12481 tggtctgtca cctcaccagc gccagcgtcg aggccggcca ggccgtccgc cgcggcgaag
    12541 tgctgggcct ctccggcagc agcggccggg ccaccgggcc gcatctgcac tggagcgtct
    12601 acctcaacgg cgcggcggtc gatcccgccc tgttcatccc gccgccagcc cgttgagcgc
    12661 ccgcgccgca gcaaacagcc cgacgctggc cgtcactgtc accaccgacc cgaagcccgc
    12721 gcaattgagt cctgccggcc cctgcggcgc gccgccgccc gcggcatcgc agaccgtcgc
    12781 cggtgccgga tagcgcaggg gctccgtcga gaacaccgcc tccacgccga acttcttctt
    12841 cccatcgcgg ggaaagccgt gggccttgcg caactgcccg cgcaccttgg ccagcagcgg
    12901 gtcctggatc gtgcgcgcca ggtcgtccac gcggatctgc gtcgggtcga tctgcccgcc
    12961 cgccgcgccg caggtcacca ccggcacgcc cgccgcgcgg cacacggcga tcatcgccac
    13021 cttcacgcgc accgcgtcga tggcatccac cacgtaatcg aacccggcac cgagcagggt
    13081 cgccgcgttc tccaccgtca cgaaatcatc cacccgcgtc accgcgcaac ccggcgcata
    13141 gccggcgatg cgcgcggcca tcgcgtccac cttggcctgg ccgagggtcg cttcggtggc
    13201 gtggatctgc cggttgagat tggactccgc cacgtggtcc agatcgatca gcgtgagcgc
    13261 gcccacgccg ctgcgggcca gcgcctctgc cgcccaggaa cccacgccgc cgatgcccac
    13321 caccgccacc cgcgccgccg ccagacgggc ccgcccagcc tcgccgtaga ggcgcgcgat
    13381 gccaccgaag cggcgcggat cctccgcgcc ccctgaaaac gcgtccgtca tctcaggcgc
    13441 tgcgggaatc gagccacaca tccaggccct gggcgttcaa ctccacgtcc accgccagca
    13501 gacggcgcca gtcggcttcc ggcagacgcc agccggcgtt gcgggcctcg tccgccacca
    13561 tcgcccacag gtccgcctcg atggcctgct ggcgcggctc gccaccgggc aggccggcat
    13621 gacgctcggc gatggccaca tgggtgtcga tcaggcgatg cagatccgcc gccaggcgcg
    13681 gcacctcggt gagctgggcg taatgggtca gatacatggc caccggctgc tccgcctcca
    13741 tgcgcgcaac ggacgcatgc atcgccaccg gatcgaactg caccggcgag gtggtcggga
    13801 agatgctcgc ccggccgtcc acgtccaggt ggcggtagga cacaccaaaa gtatcgccgg
    13861 tgaagaagct gcggctgccg gtgtcgaagt aacacacgtg atggcgcgca tggcccggcg
    13921 tatccagcac gcggatcacc cgcccggcga gggacagctc gagcccgtcg gtggcttcca
    13981 cgatgcgcgc caccggcacc gggatcaact cgccgtagag ctccttggcc cgctccggcc
    14041 catacaccgc cgacaccccc gcaaacagct tggacggctc cgccatgtga cgcgcaccgc
    14101 ggggatgcac caccagcttc gcgttcggga acgccttcat gaaggccccc gcgccgcccg
    14161 catgatccag gtggatgtgg gtcagcagca cgtaatccac cgcctccggc ttcaggcccg
    14221 tggccttcag cgcctcttcc acatgcacca gcgccgcatt gttacccgta tccaccagcg
    14281 ccgcccggcc gccttcctcg atgaggtgga tcgccgcgat ccccgtgccc gtgtaacccg
    14341 aatccacagc gtagatgccg tgcccatagg aagtcagccc ctgcatgctc atccctttga
    14401 aaaaagaacg gattctagcc cgcccctgta tgaatgcgac cgttgcgttg catcaatgtt
    14461 tcagtcgccc gaaaggcgtc caatggactt aaaaagcatt atcacaacac aaggagggaa
    14521 gcatcatgtt gacgatccag gactgcatcg agctatccga tctcaccgag gacgaagtcc
    14581 tcgccatcgc cgagcacgaa cacctgccgg agatgctggc cgtggaaatg ggctgctatc
    14641 tctgccacac cccggacggc gacaagcgca tccgccgcat gatcgccgac gacatccgcc
    14701 acgcccacgc cctgggcgat gtgcagcgca ccgcgctcct caaatccgtg ctcaagcact
    14761 acgtgcagga gcatcacggc aacgcctgag cgttcatcca gtcgccacgg agatatcggg
    14821 ttggcataat ccgccaaccc gaccgatcat gacatcccca tggcaatcct tcgatgcaat
    14881 cattgcggcc acgtcgccga gacccttgtc gacgccaacg gccagaacat gccctgcccc
    14941 cgctgcatca cgccggcgcc ggtctacgac accgtcgtct tcatccgcaa ggtgctcgac
    15001 cagtatttcc agctgcggga cgaactcaac cggctcaagg caggcgcttc cgccatgatg
    15061 caggcggagc cggcccgcct cgccaccgcc acggcgacga cggcagcgcc gtgcgatccg
    15121 cctcccgtcg cccgcccgcc agccggcgcc cgacccaacc agttcgccct caacagtcag
    15181 ttcgccgcca tcgagcaatg gttcctgcgt cgtcagatcc agatccgccc ccccaccgac
    15241 gcgcccgaac tcagccccgc cttcgaacgc accgccgccg acctgggccg ataccacacc
    15301 ctgctgcgcg acttctttga acgcatcccc gccacgccca ccccggacga caaccgcctc
    15361 cacctcgacc tgaacgcccg caacgcccag gagatcggtg acctggacgc cgtcttcgcg
    15421 cgcctgcacg gcagcgcctt cctctcccat tacaccctcg acaagcccga ccgcagtctc
    15481 cacgccgcct tgcccgccga ccccgccatc ctgcgcttcc tctcgggcga atggctggag
    15541 tggtacagcc tcggccgcct cgccgagcac ttccgcaaaa aaggcctgcg cgccgccggc
    15601 gcccgcgggc tgaccgcact cctggccgac ggcgacgaac ggccccttga cgtcttcttc
    15661 ctcgtcaatg gcaacacgcc catctgcctc gcctgccgca ccggcgacgt ccgcccggac
    15721 atcgacaccc tcctgcgtct gcgcaagcgc ctcggcatcc cccgcggcca gttcatcgtc
    15781 tgcagcccca ccctcgccga ggagcaggcc gagcacctca gcaccgccca cgaactcagc
    15841 tgcgtcaccc cggccggcct catcacgcac atcgaacgcc tcttctgaaa ccggcgtgcg
    15901 ggcgccgccg gtcgccagcc cgccaccaga ttggcataat cgggcctttc caccgagtcc
    15961 cacgccgatt ccgccatggc cctcctccgt tgcaaccagt gcggccacgt cgccgagccc
    16021 gccgccgaca ccgtcggcca gcatctgccc tgcccccact gcggcaccag cgtcccggtc
    16081 tacgacaccg tcctcttcgt gcgcaaggtg ctccagcaat acttcgccct ccgcgacgag
    16141 aacacccgcc tgaaggacgc cctcgccgct gcgccggcca cgacggagga agcgcccgcc
    16201 gcccccgcca gcgccccgcc cctctccgac atcgacctgc acaactccag ccacttcgcc
    16261 agcgaagccc agcacgccgc catccagcag tggttcgccc ggcgccagat cgaggcccgc
    16321 cccaaccggg acgccgtcga caccaccggc ttctacgacg agatcgccgt tgccctcggc
    16381 gatcactacg agctctaccg ggaagtgctc gacaagatcc gctggggcca caaaaaggac
    16441 atcccccagt tcagcctcaa cctcaccgag cgcagccaga aggacggcca ggccctcaat
    16501 gccttctgcc gccagctcca cgacagcgcc ttcctgtcca agtacttcta ccagaagcag
    16561 gacaagatca tccgcgcctc cgtccagacc gcccccgcca tccgcgcctt ctttgccggc
    16621 gaatggctgg agtggtacgc cctgatgaag ctcctcgcct ttttccgcga ccgtggcctg
    16681 ggcttctcct gcacccgcaa ccttcacatc accttcgcca acgaagacct gcacgaactc
    16741 gacgtcttct tcctgctgga cgacggcacg ccggtctgcg tcgaatgcaa gactggcgaa
    16801 ttccgcgccg acatcgacaa gatgctgcgc ctgcgcaagc gcctcggcat cgaccgcagc
    16861 cagttcatcg tctgcgccct cggcctcagc gacgaacagg ccgccggcct cagcggcacc
    16921 tacgagctca gcttcgtcac ccctgccggc ctcctccccc atctcgcccg actcttctga
    16981 cgccccccgc ggaccggggt aaaatccggt ccccatgacc ccccgcgccc gcgaactcct
    17041 ctcccacgtc ttcggctaca gcggcttccg cggcccccag gaagccatcg tcgagcacgt
    17101 ggccgcgggc ggcgacgccc tcgtgttgat gcccaccggc ggcggcaagt ccctctgcta
    17161 ccagatcccc gccctgctgc ggcccggcgt cgccatcgtc gtgtcgcccc tcatcgccct
    17221 catgcaggac caggtcagcg ccctcaagga agtgggcgtc gccgccgact tcctcaactc
    17281 cagcctcgcc ttcgagcagg ccatggagat cgagcgcgcc ctcctcgccg gcgccctcga
    17341 cctgctctac gtcgccccgg aacgcctcgt caccccgcgc ttcctcgacc tcctcgacca
    17401 cctgcgcgaa gccggccagc tcgccctctt cgccatcgac gaagcccact gcgtgtccca
    17461 gtggggccac gacttccggc ccgaatacct gcagctctcc ctgctccccg agcgctaccc
    17521 ccacatcccg cgcatcgccc tcaccgccac cgccgacgcc cagacccgcc gcgagatcgc
    17581 cgagcgcctg tgcctcaccg acgcccgcga attcgtctcc agcttcgacc ggcccaacat
    17641 ccgctacacc atcgtcccca aggacaatcc ccgcgcccag ctcctcgcct tcatccgcga
    17701 cggccaccag ggcgaagccg gcatcgtcta ctgcagctcc cgcaaaaagg tggacgaaac
    17761 cgccgcctgg ctcctcgaaa aaggcatcgc cgccctgccc taccacgccg gcctgcccgc
    17821 cgagcagcgc gccacccacc aggaccgctt cctgcgcgaa gacggcatcg t
//
LOCUS       DAOQMM010000075        17743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1192_length_17743_cov_55.173847, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000075 DAOQMM010000000
VERSION     DAOQMM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17743
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1192_length_17743_cov_55.173847"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLH85_12125"
     CDS             <1..519
                     /locus_tag="PLH85_12125"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNB65358.1"
                     /translation="PSRVVLVGMPGSGKTTVGRELAKRLGMRFLDTDHEIEVRTGVKI
                     PTIFEIEGEAGFRRRETQTLDALTGETGVVLATGGGAVIAAENRQMLAARGVVVYLNV
                     PPHILWERTRHDRTRPLLQVADPKARLQQLFAERDPFYREVAHIIVEGGRGTPAAMVR
                     TIEREIQELCAH"
     gene            507..1586
                     /gene="aroB"
                     /locus_tag="PLH85_12130"
     CDS             507..1586
                     /gene="aroB"
                     /locus_tag="PLH85_12130"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNB65359.1"
                     /translation="MRTLNVELAARRYPIHIGTGLLDRSDLILPFLKTPRAAVVTNDV
                     VGPLYLARFSAALRAAGVRVTEIVLPDGEAHKDWPTLNLIFDGLLGERCDRATTVIAL
                     GGGVVGDMAGFAASAYQRGVPFIQVPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     RLVLADTSVLSTLPDRELRAGVAEVIKYGLIRDLPFLEWLEANLDRLLHRDGDVLAEA
                     IERSCRNKAEVVVADETEQGERALLNLGHTFGHAIETGLGYGEWLHGEAVAAGAVMAA
                     ELSRRLGWLSTADVTRVREIHARAGLPVRGAALGADRYLELMAHDKKVSNGTLRLVLL
                     KAIGRAVVTAEAPIDAVRASIDACC"
     gene            1594..2718
                     /locus_tag="PLH85_12135"
     CDS             1594..2718
                     /locus_tag="PLH85_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNB65360.1"
                     /translation="MSVLAPYAVTEAVSRGRRIPEAPPAARGEFQRDRDRIVHSTAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSIEVAQITRTIARALHLNEDLAEAVALAHDLGHTP
                     FGHAGQEALNACMKDYGGFEHNLQSLRTVDVLEESYAAFDGLNLMFETREGILKHCSP
                     KNAELLGDVGRRFLEGRQPSLEAQLANLADEIAYNNHDVDDGLRSGLITLEQLDDVPV
                     FARNRRSVEQEYPGLSGRRLVHETIRRMINLMAVDLIRQSTTNIAASGVASTGDVASA
                     PRLLAFSADLLPDLRVLKTFLRENLYWHYQVLRMTDKARRIIHDLFGAFMNDPRLLPP
                     QYQQMARQDTPRAIADYIAGMTDRYAMKEHRRLFAVGEIH"
     gene            2953..7626
                     /locus_tag="PLH85_12140"
     CDS             2953..7626
                     /locus_tag="PLH85_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNB65361.1"
                     /translation="MDLPKQQGLYDPANEHDACGVGFIAHIKGKKSHDVVTQGLEILK
                     NLDHRGAVGADPLQGDGAGILIQIPDLLFREEMAKQGVTLPPPGDYGVGMVFLPKEHA
                     SRLACEEEIERAARAEGQVVLGWRDVPIDPAMPMSPTVKAKEPVIRQVFIGRGPDVMV
                     TEALERKLYVIRRRAANAIGSLRLKHSKEFYTPSMSARTINYKGLLLADQVGQYYLDL
                     QDARCDSALALVHQRFSTNTFPTWHLAHPFRYIAHNGEINTVRGNYNWMRAREKGTHS
                     PLLGDDLYKLWPLIYPGQSDSASFDNALELLVMSGYSLAHAMMMMIPEAWESHTLMDA
                     KRRAFYEYHAAMMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLVTDDDYVVMASES
                     GVLPIPDEKIVKKWRLQPGKMFLIDMEQGRIIDDKELKDTLAAAKPYQEWIRRINIRL
                     DACPDPAGSAAPEYAASLLDRQQAFGYSQEDIKFILEPMAKSGEEGTGSMGNDSPLAV
                     LSARPKVLYMYFRQLFAQVTNPPIDPIREQLVMSLVTFVGPRPNLLGINEINPPYRLE
                     VPQPVLTVEDMAKLREIARYTEDKFRSAELDICYPIAWGKEGVEARLASLCAEAEDAV
                     LNGSNILIVSDRKVDALNVAIPALLATSAVHQHLVAKGLRTRTGLVVETGSARETHHF
                     AVLAGYGAEAVHPYLALETLSQMAGDAETAEKYVKHFIKGVGKGLMKVMSKMGISTFM
                     SYTGAQIFEAVGLQKSLVEKYFTGTSSQVEGIGVFEVMDEAIRLHQQAFSADPVLAGM
                     LDAGGEYAFRIRGEEHMWTPDAIAKLQHSTRSGKYDTYKEYARIINDQSKRHMTLRGL
                     FEFKPAGAPVPLEEVESAKDIVKRFATGAMSLGSISTEAHTTLAIAMNRIGGRSNTGE
                     GGEDPKRFIPIAEPTKLSEVIGASRIERDIELKAGDSLRSSIKQVASGRFGVTTEYLV
                     NADQIQIKMAQGAKPGEGGQLPGHKVSEYIGFLRHSVPGVGLISPPPHHDIYSIEDLA
                     QLIHDLKNANPAAGISVKLVSEVGVGTVAAGVAKAKADHVVIAGHDGGTGASPWSSIK
                     YAGSPWELGLAETQQTLVLNRLRSRIRVQVDGQIKTGRDVVVGALLGADEFGFATAPL
                     VVEGCIMMRKCHLNTCPVGVATQDPTLRKRFSGQPEYVVNYFFFIAEEVREIMAQLGI
                     RKFDELIGRADLLDMRAGIDHWKARGLDYSRIFHLPNVPAAVPRLHTETQDHNLAKAL
                     DNQLIELAKPALEKAEAVNIELPVRNINRTVGAMLSGRVAAKYGHAGLPDDTIHIRLN
                     GTAGQSFGAFLARGVTLDLVGEGNDYVGKGLSGGRIVVRPAAGFRGETPSNIIIGNTV
                     MYGATEGEAYFAGVGGERFAVRNSGATAVVEGVGDHGCEYMTGGTVVVLGMTGRNFAA
                     GMSGGVAYVLDTDGTFESRCNMAQVALEPVEEELAARQGSESGDELEAHGRVDVRHLG
                     MADEALLKQLVENHARYTGSERAKAILADWSEYRAKFVKVMPHEYRRALAEMAAQKQQ
                     ELEAA"
     gene            7628..9091
                     /locus_tag="PLH85_12145"
     CDS             7628..9091
                     /locus_tag="PLH85_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNB65362.1"
                     /translation="MGKPTGFLEFQRLSEAYAPVEERLKHYKEFVCTLKDDEAARQGA
                     RCMDCGIPFCNNGCPVNNIIPDWNDLVFRGQWEQAIEVLHSTNNFPDFTGRICPAPCE
                     AACTLNINTDAVGIKSIEHAIIDKAWESGWIKPQPAAVKTGKKVAIVGSGPAGLAAAQ
                     QLARVGHDVTVFEKSSRIGGLLRYGIPDFKMEKSHIDRRVAQMEAEGVTFRTQVCVGA
                     KPGLTGINDDSVEVVSADQLTREFDAVILAAGSEVPRDLPVPGRELKGVHFALELLIP
                     QNKEVAGEDKNPISAAGKHVIVIGGGDTGSDCVGTSNRHGALSVTQFEVMPQPPAEEN
                     RPLTWPYWPIKLRTSSSHDEGCNREFAVSTKEFIGENGQVKALRTVQLEWKDGKPSEV
                     PGSVRELPADLVLFAMGFTNPVASLLEAFGVDKDGRGNAKASTDGVGCYATNVPKVFA
                     AGDVRRGQSLVVWAIREGRQAAREVDTFLMGSSVLPR"
     gene            complement(9213..10412)
                     /locus_tag="PLH85_12150"
     CDS             complement(9213..10412)
                     /locus_tag="PLH85_12150"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNB65363.1"
                     /translation="MKAILILNAGSSSLKFALFPLAPRLADAPRLSGQVEGIGAEPLM
                     HAKDTHSGERFTEALPIAPGTDQNTQHRNALEFVFDWIRRHSPGVDIVAAGHRIVHGG
                     DQYGAPVALTPDVVGALETLIPLAPLHQPHNLRAIKSLFTLMPDVRQVGCFDTAFHRT
                     RLPVAERFPLPRALFDEGVKRYGFHGLSYEFVSRQLPDLLGEEKAHGPIVVAHLGNGA
                     SMCALRDGLSRDTSMGFTAVDGLMMGTRTGSLDPGVILHLIEQKGMDAKALSNLVYKQ
                     SGLLGVSGISQDMRALLESDEIAAQEAIELFCYRAACMIGQLTMAAGGLDALVFTGGI
                     GEHAAPIRARIAEWLAWTGLHLYPAANQRHATRIHAKDSSVEVLVVPTNEEWMIAHHT
                     VELLGLA"
     gene            complement(10409..11821)
                     /locus_tag="PLH85_12155"
     CDS             complement(10409..11821)
                     /locus_tag="PLH85_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNB65364.1"
                     /translation="MNTPNHDFIENRTFDEIAVGDSAHLVRTLRPEDIHLFAVMSGDV
                     NPTHVDPEFARSSQFREVVGHSMWGSVIISSILGTEFPGPGTVYVGQTLKFWRPITIG
                     DTLTVTITCTHRFEHNHHLLLDCQCVNQDGLKVIDGTAEVMAPTEKIKRPRVALPEIT
                     ISDRQQRYLHALELTKGLAPIPIAVAHPCDTESLRGPLQARDAGLVIPVLVGPEAKIR
                     EVAEREHLDLNGVRIVNVPHSHASAETAVRLARTGEVEALMKGSLHTDELMSEVVAKE
                     TGLRTSRRISHVFMADVPTYPHPLLITDAAINIEPSLEDKVHIIQNAIELAIMMGVPE
                     PKVAILSAVETVNVKIRSTLDAAALCKMADRGQIKGGLLDGPLAFDNAVSLIAAKTKG
                     IRSAVAGQADILVVPDLESGNMVAKQLEYLADALMAGVVLGARVPIVLTSRADTAETR
                     TASCAIAQLMAHKRREGMRK"
     gene            12029..13396
                     /gene="glmU"
                     /locus_tag="PLH85_12160"
     CDS             12029..13396
                     /gene="glmU"
                     /locus_tag="PLH85_12160"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNB65365.1"
                     /translation="MKVIVLAAGQGKRMRSILPKVLQPLAAKPLLAHVLDTARALQAE
                     RIVVVYGHGGEVVREALDAPDLAWARQDPPQGTGHAVQQAMPHVDDGDTALILYGDVP
                     LIGEATLRRLVGATGPEKLALLTVGMDDPTGYGRILRDAAGKVTSIVEEKDATPEQRQ
                     VREVNTGILAAPVTQLRAWLARLKNDNAQGEYYLTDIIAMAVADGFEVVTTQPDAFEE
                     TLGVNNKTQLAELERIHQRAIARRLTDAGVTVIDPARIDVRGELICGRDVEIDVNCVF
                     EGRVELADGVRIGANCVLRNATLGAGTRVAPYSHIEDTTMGPACIIGPYARTRPGTVL
                     GEDVHVGNFVEIKNSVIADHSKANHLAYVGDADIGQRVNVGAGTITCNYDGANKFRTV
                     IEDDVFIGSDTQLVAPVRVARGATLGAGTTLTKDAPAEQLTVSRARQVTVPGWTRPVK
                     AAKKS"
     gene            13465..15303
                     /gene="glmS"
                     /locus_tag="PLH85_12165"
     CDS             13465..15303
                     /gene="glmS"
                     /locus_tag="PLH85_12165"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNB65366.1"
                     /translation="MCGIVTAIARRNIVPVLLEGLRKLEYRGYDSAGLAVLKTDGAPA
                     LQRLRAVGRVAELSAQADATALSASIGIAHTRWATHGVPSERNAHPHISGGLAVVHNG
                     IIENFEELRAALQAKGYVFTSETDTEAIAHLLHDTLKTVSDLYEAVRVTIAQLVGAYA
                     IGIVSEAYPDRVIVARKGSPLLLGIGEDGHYAASDTAALLQVTRKVVYLEDGDVAELT
                     LDGYRVTLADGTPVERPVNISSLSADAVELGKYRHYMQKEIFEQPQALANTLEPIAGA
                     GAISPQIFGAEAVDVLKATRRVLILACGTSYHSGFTARYWLESVARIPCTVEIASEYR
                     YRESVPDPDTLVVVISQSGETADTLAALKHAKGLGMTKTLAICNVPESAIVRESALRL
                     ITRAGPEIGVASTKAFTTQLAALALLALTLAKINGRLGAEQESGYLTQLRHLPVAVAK
                     VLELEPEIEKWAQRFAGKHHALFLGRGSHWPIAMEGALKLKEISYIHAESYAAGELKH
                     GPLALVDKEMPVIAVAPADELLEKLKSNLQEVRARGGELYVFADAGSEIPESEGVHIL
                     HMPEHYGMLSPILHVIPLQLLSYHAALVKGTDVDKPRNLAKSVTVE"
     gene            15590..16138
                     /locus_tag="PLH85_12170"
     CDS             15590..16138
                     /locus_tag="PLH85_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB65367.1"
                     /translation="MAERNRHLTETISRESGRLGRFIRQRVPDPGDAEDILQDVFFEL
                     VEAWRLPEPIEQVGAWLFRVARHRIVDRFRKRREEPLPLAPEGDDDDTEHWLAQALPT
                     TDGGPEAAHLRRQWLDAIAVALDELPPGPRDVFVAHEIDGRSFKDMAAESGVPLNTLL
                     GWKRQAVLHLRARLAPLYLDHS"
     gene            16151..16546
                     /locus_tag="PLH85_12175"
     CDS             16151..16546
                     /locus_tag="PLH85_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65368.1"
                     /translation="MMNCSTSRHPRSAARCIKIGLLVVAGVAAVTGVVMLLWNALLPQ
                     LFAGVSPIGYWQALGVLVLSRLLFGGLRGGGHGRWHAHRAQWEHLTPEEREQFKGRFH
                     RRWGCCGSADAADASAARAEPGDKSADPA"
     gene            16626..>17743
                     /locus_tag="PLH85_12180"
     CDS             16626..>17743
                     /locus_tag="PLH85_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB65369.1"
                     /translation="MSSTRTEAPPAVEPAGLPRRLRGALLRILPLLGGLHAYLGWRLL
                     PALDLGALGLSLAIAGLVLSAGLIPLGLLGRFLIPHPGWADRLSGLGGLLMGAFSTLL
                     VFTVLRDGLLWIANDPALAAPTALGVLGLAAFVTAVGYVNARRVARVRRVDLPLADLP
                     DALAGFTLVQLSDLHVGPTIKGDYVQAIVDRVNGLDADLVVLTGDLVDGRVDHLRAHT
                     APLGRLRSRHGPYMVTGNHEYYSGAAAWMREFERIGLRGLHNRHVLIPHRGARFVLAG
                     VTDYSAGVFDPAQASDPVAALAGSPPGLLRILLAHQPRSAAAAEAAGFHLQLSGHTHG
                     GQFWPWNHFVPLQQPFTAGLHRFGRMLVYTSRGTGYWGP"
BASE COUNT         2997 a         6131 c         5629 g         2986 t
ORIGIN      
        1 ccttcccgag tcgtcctggt gggcatgccg ggttcaggca agacgacggt tggtcgggaa
       61 ctggccaagc gtctcggcat gcgcttcctc gataccgacc acgagatcga ggtgcgcacg
      121 ggcgtcaaga ttccaaccat tttcgagatc gagggtgaag ccgggttccg gcgccgggag
      181 acccagaccc tcgacgccct cacgggcgaa acgggcgtcg tgctggcgac tggcggaggc
      241 gccgtgatcg cagcagaaaa ccgtcagatg cttgcggccc gcggtgtcgt cgtttacctg
      301 aatgtgccgc cccacatcct ttgggagcgc acccgccacg accgtacgcg gccgctgctg
      361 caggtggccg acccaaaggc gcgcttgcag cagctgttcg ccgagcggga tcccttttat
      421 cgagaggtgg cgcacatcat cgtcgagggc gggcggggga cccccgctgc catggtgcgc
      481 accatcgagc gcgaaatcca ggaactatgc gcacactgaa cgtcgaactg gcagcgcgcc
      541 gctatccgat tcacatcggc accggcttgc ttgatcgcag cgatctgatc cttccctttc
      601 tcaagacccc acgggctgcg gtcgtcacca acgatgtggt cgggccgctc tatctcgcac
      661 gtttcagtgc cgccctgcgt gcggccggcg tccgggtgac cgaaatcgtc ctgcccgatg
      721 gtgaagcgca caaggattgg ccgacactca acctgatctt cgacggtctg ctgggtgagc
      781 gatgcgatcg cgccacaacc gtcatcgccc ttggtggcgg agtcgtcggc gatatggccg
      841 gcttcgccgc atcggcctac cagcggggtg tgcccttcat ccaggtgccg actacgctgc
      901 tgtcccaggt ggattcctcg gtgggtggca agactgccat caaccacccg cttggcaaga
      961 acatgatcgg tgccttttac cagccgcggc tggtcctggc cgatacctcg gtgctgtcca
     1021 cgctgccgga tcgggaactg cgcgccggcg tcgccgaagt catcaagtat ggcttgatcc
     1081 gcgacctgcc ttttctcgag tggctggagg cgaatctcga ccggctgctg caccgcgacg
     1141 gggatgtgct ggccgaagcc atcgagcgtt cgtgccgcaa taaggccgaa gtcgttgtcg
     1201 ccgacgaaac cgagcagggt gagcgggccc ttctcaatct cggtcacacg ttcggccacg
     1261 ccatcgagac cggcctcggt tacggggaat ggctgcatgg tgaggcggtc gccgccgggg
     1321 ccgtcatggc tgccgaactg tcacgccggc tgggttggct gtcgacggcc gatgtgaccc
     1381 gtgtgcgcga gatccacgcc cgggccggcc tgcccgtgcg gggtgcggcg ctgggggctg
     1441 accgctatct cgaactcatg gcgcacgaca agaaggtcag taatggcacc ctccggctcg
     1501 tcctcctcaa agcgatcggc cgtgccgtgg tgacggccga agcgccgata gatgccgtcc
     1561 gcgccagcat cgacgcctgc tgctgaagcc gcgatgagtg tgctcgcccc ctacgccgtc
     1621 accgaagcgg tttcccgggg ccgcaggatt ccagaagccc cgcccgctgc acggggcgag
     1681 tttcagcgcg atcgggaccg catcgtccat tcaacggctt tccgccggct tgaatacaag
     1741 acccaggtct tcgtcaatca cgaaggcgac ctcttccgca cgcggctcac ccacagcatc
     1801 gaggtcgcgc agatcacccg gaccatcgcc cgtgcgctgc atctcaacga ggacctcgcc
     1861 gaggcggtcg cgctggccca cgacctcggc cacacgccct tcggccacgc tggccaggag
     1921 gcactcaacg cctgcatgaa ggactacggg gggttcgagc acaacctcca gtccctgcga
     1981 acggtagacg tgctggagga gagctacgcc gccttcgacg gtctcaacct gatgtttgaa
     2041 acgcgggaag gcatcctcaa gcactgttca cccaagaacg ccgagttgct cggtgacgtt
     2101 ggccggcgct tcctggaagg acgtcagccc tcgctggagg cccagctcgc caatctggcg
     2161 gatgagatcg cctacaacaa tcacgacgtg gacgatggat tgcgctcggg tctgatcacc
     2221 ctcgagcaac tcgacgatgt gcccgtcttt gcgcgcaacc ggcggtccgt cgaacaagaa
     2281 tatccggggc tctcggggcg ccgcctggtc catgagacga tccgccgcat gatcaacctg
     2341 atggccgtgg acctgatccg ccagtcgacc accaacattg ctgcgtccgg cgtcgcgagc
     2401 accggcgacg tggcctcggc gcccaggctg ctggccttca gtgccgatct gttgccggat
     2461 ctgcgcgtcc tcaagacctt tctgcgggaa aacctctact ggcactatca ggtgctgcgc
     2521 atgaccgaca aggcgcggcg catcatccac gatctctttg gcgccttcat gaacgatccg
     2581 cgcctgctgc cgccccagta tcagcagatg gcgcgccagg atacgccgcg ggcgatcgcc
     2641 gactacatcg ccggaatgac tgaccgctat gccatgaagg aacaccggcg cctcttcgcc
     2701 gtcggcgaga ttcattgagc gccgcacggt tttttcgcgc tgcaaaaaat ccttgaacca
     2761 caaggccctg cggcggtata ttcgaccctc gcccaatgtg tttgaacggt ctcctcgtcg
     2821 ggatctctcg aagccggcat gcttgcgccc gccggctttt tcacggttcg gacggggcat
     2881 tttccgcagt ttgtaaggcc ggtaaacctc cgatggaggg ggccggtgcg tgttgtgtcg
     2941 aggaaaaaca agatggatct tcccaagcag cagggactct acgaccccgc caacgaacat
     3001 gacgcctgtg gtgtcggttt cattgcacat atcaagggca agaagtctca cgatgtcgtg
     3061 acccagggtc tcgaaatcct caagaatctg gaccatcggg gtgccgtggg tgccgatccc
     3121 ctgcagggtg acggtgccgg catcctgatc cagattcccg atctcctgtt ccgcgaggag
     3181 atggccaagc agggcgtcac cctgccgccg ccgggtgatt acggcgtggg catggtgttc
     3241 ctgcccaagg agcacgcgtc ccgtctcgcc tgcgaggagg agatcgagcg tgccgcccgc
     3301 gccgaaggtc aggtcgtgct gggctggcgt gacgtgccca tcgatcccgc gatgcccatg
     3361 tcgcccaccg tcaaggccaa ggagccggtg atccgccagg tcttcatcgg tcgcggtccc
     3421 gacgtgatgg tcaccgaagc gcttgagcgc aagctgtatg tgatccgccg ccgtgcggcc
     3481 aacgccatcg gcagtctgcg cctcaagcac agcaaggagt tctacacgcc gtccatgtcg
     3541 gcgcggacca tcaactacaa gggtctgctc ctcgccgatc aggtcggcca gtactacctc
     3601 gacctccagg acgcgcgctg cgattccgcg ttggccctgg tgcaccagcg cttctccacg
     3661 aacaccttcc cgacctggca tctggcccac ccgttccgct acatcgccca caacggcgag
     3721 atcaacacgg tgcgcggcaa ctacaactgg atgcgcgctc gcgaaaaggg cacccattcg
     3781 ccgctgttgg gggacgatct ctacaagctg tggccgctga tctatcccgg ccagtccgat
     3841 tccgcgtcct tcgacaacgc cctcgaactg ctcgtgatga gcggctattc cctggcccat
     3901 gccatgatga tgatgattcc cgaagcctgg gaatcccata ccctcatgga cgccaagcgt
     3961 cgtgccttct acgagtacca cgctgccatg atggagccct gggatggccc tgccgccgtc
     4021 gccttcaccg acggccgcca gatcggtgcc acgctggacc gtaacggcct gcgtcccgcc
     4081 cgctacctgg tgaccgacga cgattacgtg gtgatggcgt cagagtccgg tgtgctcccc
     4141 atcccggacg aaaagatcgt caagaagtgg cgcctgcagc ccggcaagat gttcctgatc
     4201 gacatggagc agggccgcat catcgacgac aaggaactca aggacaccct ggccgccgcc
     4261 aagccctacc aggagtggat ccgccgcatc aacatccgcc tcgacgcctg cccggatccg
     4321 gccggttccg ccgcaccgga atacgcggcc tccctgcttg atcgtcagca agcctttggc
     4381 tactcccagg aagacatcaa gttcatcctc gagccgatgg ccaagagtgg cgaggaaggt
     4441 accggttcga tgggtaacga cagcccgctg gccgtgctgt cggcccgtcc gaaggtgctg
     4501 tatatgtact tccggcagct cttcgcccag gtgaccaatc cgccgatcga cccgatccgc
     4561 gagcagttgg tcatgtccct ggtgaccttc gtcgggccgc gtcccaacct gctcggcatc
     4621 aacgagatca acccgcccta ccgcctcgaa gtgccccagc cggtgttgac cgtcgaggac
     4681 atggccaagc tgcgggagat tgcgcgctac accgaagaca agttccgctc cgccgaactg
     4741 gacatctgct atccgatcgc ctggggcaag gaaggcgtcg aggcacgtct cgcctcgctc
     4801 tgtgcggaag ccgaggacgc ggtgctcaac ggctccaaca tcctgatcgt ttccgaccgg
     4861 aaggtggacg cgctcaatgt ggccattccg gcgctgctcg ccacctcggc cgtgcatcag
     4921 cacctggtgg ccaaggggct gcgtacccgc accggcctgg tggtggaaac cggctccgcc
     4981 cgggaaaccc atcacttcgc cgtgctcgcc ggctatggcg ccgaagcggt gcacccctac
     5041 ctggctctgg aaaccctcag tcagatggcc ggggacgccg agaccgcaga aaagtacgtc
     5101 aagcacttca tcaagggtgt cggcaagggc ctcatgaagg tcatgtcaaa gatgggcatt
     5161 tccaccttca tgtcctacac cggcgcgcag atcttcgaag cggttggtct gcagaagtcg
     5221 ctggtggaaa agtacttcac gggtacctcg tcccaggtgg aaggcatcgg cgtgttcgag
     5281 gtcatggatg aggccatccg cctccaccag caggccttca gcgccgaccc ggtgctggcc
     5341 ggcatgctcg acgccggcgg tgaatacgcc ttccggatcc gcggcgaaga gcacatgtgg
     5401 accccggacg ccatcgccaa gctgcagcac tccacccgct ccggcaagta cgacacgtac
     5461 aaggagtacg cccgcatcat caacgatcag agcaagcgcc acatgaccct gcgcggcctg
     5521 ttcgagttca agccggcggg cgccccggtg ccgctggaag aagtcgagtc cgccaaggac
     5581 atcgtcaagc gtttcgccac gggtgcgatg tccctgggct ccatctccac cgaagcccac
     5641 accaccctgg ccatcgccat gaaccgtatc ggcggtcgct ccaataccgg cgaaggtggc
     5701 gaggatccga agcgcttcat ccccatcgcc gagccgacca agctgtctga agtcatcggt
     5761 gccagccgca tcgagcgcga catcgaactc aaggccggcg acagcctgcg ctcctcgatc
     5821 aagcaggtgg cgtcgggtcg tttcggcgtg accaccgaat acctggtcaa tgccgaccag
     5881 atccagatca agatggccca gggcgccaag cccggtgaag gcggccagct gcccggccac
     5941 aaggtgtccg agtacatcgg cttcctgcgt cactcggtgc cgggcgtcgg cctgatctcg
     6001 ccgccgccgc accacgacat ctactccatc gaggatctgg cccagttgat ccacgatctc
     6061 aagaacgcca atccggcggc gggcatctcc gtcaagctgg tgtccgaagt gggcgtcggg
     6121 accgtggcgg ccggcgtggc caaggccaag gccgaccacg tggtgatcgc cggtcacgat
     6181 ggcggcacag gcgcttcgcc ctggtcgtcc atcaagtacg ccggctcccc gtgggaactc
     6241 ggtctcgccg agacccagca gaccctggtg ctcaaccgcc tgcgcagccg catccgcgtg
     6301 caggtggacg gtcagatcaa gaccggccgt gacgtggtcg tgggtgccct gctgggggcc
     6361 gacgagttcg gcttcgccac cgcgccgctg gtggtggaag gctgcatcat gatgcgcaag
     6421 tgccacctca acacctgtcc ggtgggtgtc gcgacgcagg atccgaccct gcgcaaacgc
     6481 ttctccggtc agcccgaata cgtggtgaat tacttcttct tcatcgccga ggaagtgcgc
     6541 gagatcatgg cccagctggg catccgcaag ttcgacgagc tgatcggccg cgccgacctg
     6601 ctcgacatgc gtgccggcat cgaccactgg aaggcccgcg gactggatta cagccgcatc
     6661 ttccatctgc ccaatgtgcc ggccgcagtg ccccgcctgc ataccgaaac ccaggatcac
     6721 aacctggcca aggctctgga caatcagctc atcgagctgg ccaagccggc gctggaaaag
     6781 gccgaggcgg tcaacatcga actgcccgtg cgcaacatca accgcaccgt cggcgccatg
     6841 ctgtcgggcc gcgtcgcggc caagtacggt cacgccggcc tgcccgacga caccatccac
     6901 atccgcctga acggtaccgc cggtcagagc ttcggtgcct tcctggcgcg tggcgtcacg
     6961 ctcgatctgg tgggcgaggg caacgactat gtgggcaagg gcctctccgg cggccgcatc
     7021 gtcgtgcgtc cggcggcggg cttccgcggt gagaccccga gcaacatcat catcggcaac
     7081 accgtcatgt acggcgccac cgagggcgaa gcgtacttcg ccggcgtggg cggcgagcgc
     7141 ttcgcggtgc gcaactcagg cgcgaccgcg gtggtggaag gcgtgggcga tcacggctgc
     7201 gaatacatga ccggtggcac cgtggtggtg ctgggcatga ccggccgcaa ctttgccgcg
     7261 ggcatgtccg gtggcgtggc ctacgtgctc gacacggacg gcaccttcga atcccgctgc
     7321 aacatggccc aggtggccct ggagccggtg gaagaggaac tggccgcccg tcagggttcc
     7381 gagtccggcg acgagctgga agcgcatggt cgcgtggatg tccgtcacct gggcatggcc
     7441 gacgaagcac tgctcaagca gctggtcgag aaccacgccc gctacaccgg cagcgaacgc
     7501 gccaaggcca tcctggcgga ctggagcgaa taccgcgcca agttcgtcaa ggtgatgccg
     7561 cacgagtacc gtcgcgcact ggccgaaatg gccgcgcaga aacagcaaga actggaggcc
     7621 gcttaacatg ggcaagccca ctggatttct cgagttccag cgcctctccg aggcctatgc
     7681 tccggtcgag gagcgactca agcactacaa ggagttcgtc tgtacgctga aggacgatga
     7741 agcggcccga cagggcgcgc gctgcatgga ctgcggcatc cccttctgca acaacggctg
     7801 cccggtgaac aacatcattc ccgactggaa tgatctggtg ttccgcggtc agtgggagca
     7861 ggccatcgag gtgctgcatt ccaccaacaa ctttccggac ttcaccggcc gcatctgtcc
     7921 ggcgccctgt gaggcggcgt gcaccctcaa catcaacacc gatgcggtgg gcatcaagtc
     7981 catcgagcac gccatcatcg acaaggcctg ggaaagcggc tggatcaagc cccagccggc
     8041 cgcggtcaag accggcaaga aggtcgccat cgtcggttcc ggaccggccg gtctggcggc
     8101 ggcccagcag ctcgcccgcg tcgggcacga tgtgacggtg ttcgagaaga gcagccgcat
     8161 cggcggtctg ctgcgctacg gcattcccga cttcaagatg gagaagtccc acatcgaccg
     8221 tcgcgtggcg cagatggagg cggagggcgt gaccttccgc acccaggtct gcgtgggggc
     8281 caagcctggc ctgaccggca tcaacgacga ttccgtcgag gtggtcagtg ctgaccagct
     8341 tacccgcgag ttcgacgcgg tgatccttgc tgcgggctcc gaagtccccc gcgacctgcc
     8401 ggttcccggc cgcgagctga agggcgtcca ctttgcgctg gagctgctga tcccgcagaa
     8461 caaggaagtc gccggcgaag acaagaatcc gatctcggcg gccggcaagc acgtcatcgt
     8521 gatcggcggc ggcgataccg gttccgactg tgtcggcacc tccaatcgtc acggcgccct
     8581 gtcggtcacc cagttcgagg tgatgcccca gccgccggcc gaggaaaacc gtccgctcac
     8641 ctggccctac tggccgatca agctgcgcac gtcctcgtcc cacgacgaag ggtgcaaccg
     8701 cgagttcgcc gtgtccacca aagaattcat cggtgagaac ggccaggtca aggccctgcg
     8761 taccgtccag ctggagtgga aggacggcaa accctccgaa gtgccaggtt ccgtgcgcga
     8821 actgccggcg gatctggtgc ttttcgcgat gggcttcacc aacccggtgg cgtccctgct
     8881 ggaggccttc ggtgtcgaca aggacggccg cggcaacgcc aaggcgagca ccgatggcgt
     8941 cggctgctac gccaccaacg tgcccaaggt cttcgctgcc ggtgacgtcc ggcggggcca
     9001 gtcactggtg gtgtgggcga tccgcgaagg tcgtcaggcc gcccgggagg tggatacctt
     9061 cctgatgggt tcttccgtcc tgccccgctg aggattcgcc cccggatgcg ggggcgcttc
     9121 cggggtttcc ggcacttaga cggtgtcggg gaccccagtg aaaaggcccg gtcttgaacc
     9181 gggccttttc atgtctggac gtccgttcgg gcttaggcga gtccgagcag ttccaccgtg
     9241 tggtgggcga tcatccattc ttcgttggtg gggaccacca gcacttccac gctgctgtcc
     9301 ttggcgtgga tgcgcgtggc gtggcgctgg ttggcggccg ggtagaggtg caggccggtc
     9361 cacgccagcc attcggcaat gcgcgcgcga atcggcgccg cgtgttcgcc gatgccgccg
     9421 gtgaacacca gcgcatccag accgccggcg gccatggtga gttggccgat catgcaggcc
     9481 gcacggtagc agaacagttc gatggcctcc tgggcggcga tctcgtcgga ttcgagcagg
     9541 gcgcgcatat cctggctgat gccggagacg ccgagcaggc cggactgctt gtagaccaga
     9601 ttgctcaggg ccttggcgtc catgcctttc tgttcgatga ggtgcaggat cacgcccgga
     9661 tcgagggacc cggtacgggt gcccatcatc aggccatcca cggcggtgaa gcccatgctg
     9721 gtgtcacggg agagtccgtc gcgcagggcg cacatgctcg cgccgttacc caggtgggcg
     9781 acgacgatgg ggccgtgggc cttttcctcg ccgagcaggt cggggagctg gcgcgacacg
     9841 aactcgtagg acaggccgtg gaagccgtag cgcttgacac cttcgtcgaa cagcgcgcgg
     9901 ggcagcggga agcgctcggc caccggcagg cgggtgcgat ggaaggccgt gtcgaagcag
     9961 cccacctggc gcacatcggg catcagcgta aagagcgact tgatggcccg caggttgtgg
    10021 ggctggtgca gcggcgcgag gggaatcagg gtttccagcg caccgaccac gtccggcgtc
    10081 agcgccacgg gcgcgccgta ctggtcgccg ccatggacga tgcggtgccc ggcggcgacg
    10141 atgtccacgc cggggctatg gcgacggatc cagtcgaaga cgaattccag cgcgttgcgg
    10201 tgctgggtgt tctggtcggt gccgggggca atgggcaggg cttcggtgaa gcgttcgccg
    10261 gagtgggtgt ccttggcgtg catcagcggc tcggcgccga tgccttccac ctggccggac
    10321 aggcgcggcg cgtcggcgag ccgcggggcc agcgggaaga gggcgaactt gaggctggac
    10381 gagccggcgt tgaggatcag gatggctttc atttgcgcat tccttcgcga cgcttgtggg
    10441 ccatgagctg ggcgatggcg caggacgcgg tgcgcgtctc ggcggtgtcg gcccggctgg
    10501 tgagcacgat gggtacgcgg gcgccgagca ccacaccggc catcagcgcg tcggccaggt
    10561 attcaagctg cttggcgacc atgttgccgg actcgagatc gggcaccacc aggatgtcgg
    10621 cctggccggc gacggcggag cggatgccct tggtcttggc ggcgatcagg gagacggcgt
    10681 tgtcgaaggc cagcgggccg tccagcagac cgcccttgat ctgaccccgg tcggccatct
    10741 tgcacagggc ggcggcgtca agggtggagc ggatcttcac gttcaccgtt tcgaccgctg
    10801 agaggatggc gaccttgggc tcgggcacgc ccatcatgat ggccagctcg atggcgttct
    10861 ggatgatgtg caccttgtct tccagggacg gttcgatgtt gatggccgcg tcggtgatga
    10921 gcagcgggtg cgggtaggtg ggcacgtcgg ccatgaagac gtgggagatg cgccgcgagg
    10981 tgcgcaggcc ggtctccttg gcgacgactt cgctcatcag ctcgtcggtg tgcagggagc
    11041 ccttcatcag cgcctcgacc tcgccggtgc gggcgaggcg cacggcggtc tcggcggagg
    11101 cgtggctgtg gggcacgttg acgatgcgta cgccattgag atccagatgc tcgcgctcgg
    11161 ccacttcacg gatcttggct tcggggccga ccaggaccgg gatcaccaga ccggcatccc
    11221 gcgcctgcag cgggccgcgc agggattcgg tgtcgcaagg gtgggctacg gcgatgggga
    11281 tcggggccag gcccttggtg agttccagcg catgcaggta gcgctgctgg cggtcggaga
    11341 tggtgatctc aggcagggcg acgcgggggc gcttgatctt ttcggtgggg gccatcacct
    11401 cggccgtgcc gtcgatgacc ttgaggccat cctgattcac gcactggcaa tccagcagca
    11461 ggtggtggtt gtgctcgaag cggtgggtgc aggtgatggt gacggtcagc gtgtcgccga
    11521 tggtgatggg cctccagaat ttcagcgtct ggccgacata caccgtaccc ggccccggaa
    11581 actcggtgcc gaggatggaa gagatgatca cgctgcccca catggagtgg ccgaccacct
    11641 cacggaactg gctggaacgg gcgaattcgg ggtccacatg ggtcgggttc acgtcgccgg
    11701 acatgaccgc gaaaagatgg atgtcttccg ggcgcagggt gcggacgaga tgggcgctgt
    11761 cgcccaccgc gatctcgtcg aaggtccggt tttcgatgaa gtcgtgattg ggggtgttca
    11821 tggtggttct ctcggttttg caacgcagca agtacaagct tacggtagtg tgctactaaa
    11881 ctttagtatt aggccagtgg cgtgttgcat cgcagcgaca aagggctgtt gtgacaagtt
    11941 gcgcactcgt ggtgcaggcc acaaatcgat gcaaatgctc cgtgctacac tcgcgcgcat
    12001 tccataccga atgcatccgg aagactccat gaaggttatc gtcctcgccg ccggtcaggg
    12061 caagcgcatg cgctccatcc tgcccaaagt cctgcaaccc ctggccgcca agcccttgct
    12121 cgcccatgtg ctcgatacgg ctcgcgcact ccaggccgag cgcatcgtcg tggtgtatgg
    12181 ccatggcggc gaagtcgtgc gtgaggcgct ggatgcgccc gatctcgcct gggcccgtca
    12241 ggatccgccc cagggcaccg gccatgcagt gcagcaagcc atgccccatg tggatgacgg
    12301 tgacaccgcc ctcatcctct acggcgacgt gcccctgatc ggcgaggcca ccctgcgtcg
    12361 cctggtgggc gccaccggtc ccgagaagct ggccctgctc actgtgggca tggacgatcc
    12421 caccggctac gggcgcatcc tgcgagacgc tgctggcaag gtgacgtcca tcgtcgaaga
    12481 gaaggacgcg acgcccgagc agcgtcaggt gcgtgaagtg aacaccggca tcctcgccgc
    12541 gccggtcacc cagttgcgcg cctggctcgc ccggctgaag aacgacaacg cccagggcga
    12601 gtattacctt accgacatca tcgccatggc cgtggccgac ggcttcgagg tcgtcaccac
    12661 ccagcccgac gccttcgagg aaaccctcgg cgtcaacaac aagacccagc tcgccgaact
    12721 cgagcgcatc caccagcgcg ccatcgcccg tcgtctcacc gacgccggtg tcaccgtcat
    12781 cgaccccgct cgcatcgacg tgcgtggcga gctgatctgc ggccgcgacg tggagatcga
    12841 cgtcaactgc gtcttcgaag gccgggtcga gctcgccgac ggcgtgcgca tcggcgccaa
    12901 ctgtgtgctc cgcaacgcca ccctcggcgc cggcaccagg gtcgcgccct attcccacat
    12961 cgaagacacg accatggggc ctgcctgcat catcggcccc tacgcgcgta cccgtcccgg
    13021 caccgtcctg ggcgaagacg tgcatgtcgg caacttcgtc gagatcaaga acagcgtgat
    13081 cgccgaccac tccaaggcca atcacctcgc ctacgtgggc gatgcggaca tcggccagcg
    13141 ggtcaatgtg ggcgccggca ccatcacctg caactacgat ggcgccaaca aattccgcac
    13201 cgtcatcgag gacgacgtct tcatcggctc cgatacccag ctcgtcgccc ccgtccgcgt
    13261 ggcccgcggc gccaccctgg gcgctggcac cacgctgacc aaagatgcac ccgcggagca
    13321 gctcacggtt tcgcgcgcgc gtcaggtcac cgttcccggc tggacccgcc cggtcaaggc
    13381 cgccaagaag tcctgaaccg tttagacatt tccggcggcc cctgcagcgg gccgtcttca
    13441 cattgaacga aaggcattca caccatgtgc ggcatcgtca ctgccatcgc ccgtcgcaac
    13501 attgtccccg tcctgctcga aggcctgcgc aagctcgaat atcgcggcta cgattccgcc
    13561 ggcctcgccg tgctcaagac cgacggtgcg ccggccctgc agcgcctgcg cgccgtgggc
    13621 cgcgtcgccg aactgtccgc ccaggccgat gccaccgccc tgagcgcctc catcggcatc
    13681 gcccacaccc gctgggccac ccacggcgtg ccctccgagc gcaacgccca cccccacatc
    13741 tccggcgggc tggccgtcgt ccataacggc atcatcgaaa acttcgagga actgcgcgcc
    13801 gcgctgcagg ccaagggtta cgtcttcacc tccgagaccg acaccgaggc catcgcccac
    13861 ctcctgcacg acaccctcaa gacggtctcc gatctctacg aggcggtccg cgtcaccatc
    13921 gcccagctcg tcggcgccta cgccatcggt atcgtcagcg aagcctatcc cgaccgcgtc
    13981 atcgtcgccc gcaagggctc gcccctgctc ctgggcatcg gcgaagacgg ccactacgcc
    14041 gcctccgaca ccgccgcgct gctccaggtc acgcgcaagg tcgtctacct cgaagacggc
    14101 gacgtggccg agctgaccct cgacggctac cgcgtcaccc tcgccgacgg cacgccggtg
    14161 gagcgcccgg tcaacatctc cagcctgtcc gccgacgccg tcgaactggg caagtaccgc
    14221 cactacatgc agaaggagat cttcgagcag ccccaggccc tcgccaacac gctcgaaccc
    14281 atcgccggcg ccggcgccat cagcccgcag atcttcggtg ccgaggccgt ggacgtcctc
    14341 aaggccaccc gccgcgtgct gatcctcgcc tgcggcacca gctaccactc cggcttcacc
    14401 gcccgctact ggctcgaatc cgtcgccagg atcccctgca ccgtcgagat cgcctccgaa
    14461 taccgctacc gcgaatccgt gcccgatccc gacaccctgg tcgtcgtcat ctcccagtcc
    14521 ggtgaaaccg ccgacaccct ggccgccctc aagcacgcca agggcctggg catgaccaag
    14581 accctggcca tctgcaacgt gcccgagtcc gccatcgtcc gcgaatccgc cctgcgcctc
    14641 atcacccgcg ccggccccga gatcggtgtc gcctccacca aggccttcac cacccagctc
    14701 gccgccctgg ccctgctggc cctcaccctc gccaagatca acggccgcct cggcgccgag
    14761 caggaatccg gttacctcac ccagctgcgc cacctgcccg tggccgtggc caaggtgctg
    14821 gagctcgaac ccgaaatcga aaagtgggcc cagcgtttcg ccggcaagca ccacgccctc
    14881 ttcctcggcc gcggcagcca ctggcccatc gccatggaag gcgccctcaa gctcaaggaa
    14941 atctcctaca tccacgccga aagctacgcc gccggcgaac tcaaacacgg ccccctggcc
    15001 ctggtggaca aggaaatgcc cgtcatcgcc gtcgccccgg ccgacgaatt gctcgaaaag
    15061 ctcaagagca atctccagga agtccgcgcc cgcggcggcg aactctacgt cttcgccgac
    15121 gccggctccg agattcccga atccgaaggg gtgcacatcc tgcacatgcc cgaacactac
    15181 ggcatgctct cccccatcct ccacgtcatc ccgctgcaac tcctcagcta ccacgccgcc
    15241 ctggtgaagg gcacggacgt agacaagccg cggaatctgg cgaagtcggt gacggtggag
    15301 tgaagcgggg gcaggaattg acgttcgccc tttgcaaata aggtttgtcg gacgcagcag
    15361 gaaacgaagg ggccatctgg cccctttgtc gtttctcgaa ggggcgttgt acgcgaggcg
    15421 aggccccggc catcccgcgt gtgaagcaat tccgccacgc gtaccccctc acccggcgtc
    15481 gcgcttcaac tatttttccc ccgccccaca atatccacgg ctcccgttcg tctaccgtgg
    15541 catggactct tcgttttcac tgccccggtc ccgatccggg actgccccga tggccgagcg
    15601 gaatcgacat ctgactgaaa ccatctcccg tgaaagcggg cggctggggc gtttcatccg
    15661 tcagcgggtg cccgatccgg gcgatgcgga ggacatcctg caggatgtct tcttcgagct
    15721 ggtcgaagcc tggcgcttgc cggagcccat cgagcaggtg ggggcctggt tgttccgggt
    15781 ggcgcggcac cgcatcgtgg atcgtttccg caagcgtcgc gaagagccgc tgccgctcgc
    15841 gcccgagggg gacgacgacg ataccgagca ctggctcgcg caggccctgc cgaccaccga
    15901 cggcggcccg gaggcggcgc atctgcggcg tcagtggctc gacgccatcg ccgtcgccct
    15961 cgacgaactc ccgcccggcc cgcgcgatgt cttcgtcgcc cacgaaatcg acgggcgcag
    16021 cttcaaggac atggctgccg aaagcggcgt gcccctcaac accctgctcg gctggaaacg
    16081 ccaggccgtc ctgcacctgc gcgcacgtct ggcgcctctt tacctcgatc actcctgact
    16141 ggaaatcatc atgatgaact gctccacctc ccgccacccc cgcagcgccg cccgctgcat
    16201 caagatcggc ctgctcgtcg tggcgggcgt cgccgccgtc accggtgtcg tcatgctgtt
    16261 gtggaatgcc ctgctgcccc agctcttcgc cggcgtctcg cccatcggct actggcaggc
    16321 cctgggcgtg ctggtgctca gccgcctgct cttcggcggc ctgcgtggcg gcggccacgg
    16381 gcgctggcat gcccaccggg cgcagtggga acacctcacg ccggaagaac gggaacagtt
    16441 caagggacgc ttccatcgcc gctggggctg ctgcggatcg gcagacgcgg ccgacgcgtc
    16501 cgcagcccgc gccgagcccg gcgacaagtc cgccgatccg gcctgacccg acacgccttc
    16561 caaacgggcc ttgcgcggcg gcatcgccgc ggcaaagccg cctcgcccac cgtcccaggt
    16621 cttccatgtc ctccacccga accgaagcgc cgccggccgt cgagcccgcg ggcctgcccc
    16681 gtcgtctgcg cggcgccctg ctgcgcatcc tgccgctgct gggcggcctg cacgcctacc
    16741 tgggctggcg tctgctgccc gccctcgacc tcggcgccct cgggctgagt ctcgccatcg
    16801 ccggactggt cctgtccgcc ggcctgatcc ccctgggcct gctcgggcgc ttcctcatcc
    16861 cccatcccgg ctgggccgac cgtctcagcg ggctgggcgg gctgctcatg ggggccttct
    16921 cgaccctgct ggtgttcacc gtgctgcgcg atggcctgct gtggatcgcg aacgatccgg
    16981 ccctcgccgc gcccacggcc ctcggcgtgc tggggctcgc cgcattcgtc accgcggtcg
    17041 gctacgtcaa cgcccggcgc gtcgcccgtg tgcggcgcgt ggacctcccc ctggccgatc
    17101 tgcccgatgc gctggcgggc ttcaccctcg tccagctcag cgacctccac gtgggcccca
    17161 ccatcaaggg cgactacgtg caggccatcg tggaccgggt caatgggctc gatgcggacc
    17221 tcgtcgtcct caccggcgac ctggtggacg gccgcgtcga ccatcttcgc gcccacaccg
    17281 cgcccctcgg gcgactgcgc agccgccatg gcccttacat ggtcaccggc aaccacgagt
    17341 actactccgg cgccgccgcg tggatgcgcg aattcgagcg catcggcctg cgcggcctgc
    17401 acaaccgcca cgtgctcatc ccccaccggg gcgcccgctt cgtgctggct ggcgtcacgg
    17461 attactccgc cggcgtcttc gatcccgccc aggccagcga cccggtggcg gccctggccg
    17521 gcagtccgcc cggcctgctg cgcatcctgc tcgcccacca gccccgttcc gcggccgcgg
    17581 cggaggctgc cggcttccat ctccagctct ccggccacac ccacggcggc cagttctggc
    17641 cgtggaacca cttcgtgccc ttgcagcagc ccttcaccgc cggcctgcac cgctttggcc
    17701 ggatgctggt ctataccagc cgcggcacgg gctactgggg gcc
//
LOCUS       DAOQMM010000076        17615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1214_length_17615_cov_43.357118, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000076 DAOQMM010000000
VERSION     DAOQMM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17615
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_17615_cov_43.357118"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1120)
                     /locus_tag="PLH85_12185"
     CDS             complement(248..1120)
                     /locus_tag="PLH85_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB65370.1"
                     /translation="MRRPSATLHVPSPVLGACVMAGVERDTRGCTLTDRERFNFYPAS
                     PMASISWIFEGALHLMEDDGGAGGASTLTGLGPALPRLILAGPHTRPTASWSPGSVHA
                     LMVGIYPEAMARLLGRPVAAWLNRTVPLADVAPPALLDACNAVFGAPDAPFAKLETTL
                     RALWNLPAQGSPIPYLGDWVRGLATRATHSGAGCSLRQWQRRIRAWTGQSQRDLEVFV
                     RIEEAFVRRIAAGPAPNLAMIAAASGYADQSHMGRDIKRVTGVAPARFGERMAADESF
                     WYYRLIADAIAQQG"
     gene            1167..2261
                     /locus_tag="PLH85_12190"
     CDS             1167..2261
                     /locus_tag="PLH85_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB65371.1"
                     /translation="MAAVMFRTPIMPKTHFHGRLSHRLRSLATAACIATSLAARRLCG
                     RRRVASWSFLFEYGTLYIRAQFNHAFRLKDDIAASRAYFDSLYSVFETYPDVTVRETG
                     PGEPRGHWFLPDNRRHDITLLHFHGGGYTFYAGVSHHFARILAHSLGVAVFAPDYRLT
                     PEHPHPAQLEDGLAAYRHLLATGHTPERIVLCGDSAGGHLALMTLAHLAGAGLPRPRL
                     TLGISPWTDIGRRGASQFGNDPYDLVQGYMTLQFADWLKGGQDWRTDDLSPIHHDYRG
                     LGPLYLQAGGREILVDMIRDFAASVAGRGGQVRLDVWPDMNHEFHGYGDHLPESREAL
                     ARLERAIAWALQPDTPFVADTLTETDTLTS"
     gene            2433..5240
                     /locus_tag="PLH85_12195"
     CDS             2433..5240
                     /locus_tag="PLH85_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65372.1"
                     /translation="MPTSRHRTVALSALWRLGARCLLLLALGFTAQSVRAQTCSLPAN
                     WPSGLPGSCQCDSFNRASLNPSTIFSSNWIATTSDTTGLLPRIVNSGYLRLTDNTGQN
                     AKSATVPAAYPAAGNYISVEFRHYAYNGSGADGMTMVLSDYAVPAQPGAPGGSLGYAQ
                     NASQVGFAGGWIGIAFDEFGNYQNPTENRVGGPGFIAESVGVRGSGSGSSGYAWLTGT
                     NSLTGSNTIDNRTSTSPSRGYLYQVIVDARNYTSSNRTALFAINRDTTATGNSYASFV
                     AQFDVFARASSLGVSQSNVPTNWQISFTGSTGGSTNIHEIGALKICAQSADPPTGGAL
                     GGFNAIDSTYVRGNVNALQGHLFTKLAGTAFQLDIAALNSSSSGISSNYVLTGTKTVA
                     ISLIDDSAGTSCNASASACTACSKPVIATQNLVFNSSDQGYKTSGNFTVNGAYSRVIA
                     KMTDGTATGCSTDAFSIRPTSVSAVSATASNAALTGTPVFKAGTDAFTMTATVPVTGY
                     TGTLKLNTTYLQALPTGATAGALNPLSFTAATSGAGSSTATTSFTYSEAGAFKLLGTG
                     SNHALYDDSWTTVDQGSAGDCVAGSYSNTVDSSGKFGCLFGITADTATMGRFTASKFA
                     VTSSALTAACTASGKTAFTYMGQPFASLAVTVQAQNGAGIVLANYDSALLSSLAGVSW
                     VAEDSNSGTNLGSRMSVPAPSKTWNAGQYVVSTSAASFARAASPDGSFENLQLGVAVS
                     DPDGALLSNADFNPAGTGACSGTGCTAKAVGGTTALRFGRLALTNAYGWEKLRLPVAL
                     QAQYWNGSDFILNGDDSCTQFAATDFVLSGFTGLAACDTAFTANNSARLTSGAGQLVF
                     AAPNKTGSLTVSPTLGSVASGTTCTGSLPTSAAAATAASKTWLQGRWKGSATYTANPA
                     AKVAFGLHRGSDKIIQIREFY"
     gene            5343..7094
                     /locus_tag="PLH85_12200"
     CDS             5343..7094
                     /locus_tag="PLH85_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNB65373.1"
                     /translation="MNRPQRIRLGEVLLQQNLLTQAQLDTALEEQRRSGRKLGRIFVE
                     SGYVTEESLGTALARQLNIAFIDLRSHSPQAETVQLLSEALARRFRAIVLDDTGRALR
                     IGMTDPTDLFAYDELARVLKRELELAVVGETALLAALDRLYHRGEEINGLAKALTAEL
                     TEDASDFAELFGSGGGQEDAPVVRLLQTVFEEAVRLRASDIHVEPQERTLRIRFRIDG
                     VLYVQTEADVKIAPSVALRLKLMSGLDISEKRLPQDGRFNMTIRGAPVDVRISTLPSQ
                     YGESVVMRLLNQAAGLVSLDRIGLPDDMLVRLRRAAARPSGMILVTGPTGSGKTTTLY
                     SVLAELNSTERKIITVEDPVEYRLPGINQVQVQDKIDLSFERVLRAALRQDPDVILVG
                     EMRDKATAEIGMRASMTGHLVLSTLHTNDALSTPIRLLDMGVPRFMVAMSVQLVIAQR
                     LIRKLCTNCAEPLPLQPHERAWLRLELGSEVDAHSFRHGRGCHLCNNQGYSGRMGIYE
                     FLEMTPALVDAASHEDNRAFLEAGRAQIRGRTLRAGAMRLAAQGITSVAEAMRVANAQ
                     VEDEPDVPELEPVTRDA"
     gene            7087..8322
                     /locus_tag="PLH85_12205"
     CDS             7087..8322
                     /locus_tag="PLH85_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB65374.1"
                     /translation="MPEFAYTARDGSGAALAGRLEAGSAEAAAEQLLARGVTPVSVTP
                     ARADAAELLNQWLGRLGSTTVPTEALMLFSRQMHTLLKSGVPIMRALRGLQESAPDAR
                     LAEAVGEVRQSLESGRELASALARHPRVFSPFYVAMIRIGEVTGRLDEIFVRLFHHLE
                     FDKYMRDQVKAALRYPSFVIAVMIIALLVVNIWVIPAFAKVYEGFDAQLPILTRVLVG
                     VSAFVKNWWFLLIGGAAAMAFGFRAWVATAAGRLEWDRIKLRLPIMGKIILKATLARF
                     ARSFALAVRSGVPIVQALSVVANTVDNDHIASKIEGMRDGLERGESVLKGAIAAGIFT
                     PVVLQMIAVGEESGSLDDLLDEVGVMYQREVEFELKTLSSQIEPILIVFLGILVLVMA
                     LGIFLPIWDLGRVAMKGNG"
     gene            8325..8828
                     /locus_tag="PLH85_12210"
     CDS             8325..8828
                     /locus_tag="PLH85_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65375.1"
                     /translation="MRPTLRRRTGITTIEFAACLALLAVFAAFLLNRLAAYEELAEKT
                     TVDLTISQIRDGIRHDLIRALLAGKRPVVNEMLERNPVSWLAQPPEAYMGEFGSLPGQ
                     ARPGAWYYVSKTHELVYVPRHSAHLNTPSSDPVRLRWRLLPAANGSGMDALEPVRIDA
                     QPAYLWF"
     gene            8940..9347
                     /locus_tag="PLH85_12215"
     CDS             8940..9347
                     /locus_tag="PLH85_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB65376.1"
                     /translation="MKQQSGFTLIELVVVIVILGILAAVAVPKFVDLSTDAGDAAAAG
                     VAGAITSASSLNYATSVARGASGTGVLQVKSGGVCSTLAGSLLTNGVPTNITIAGGPL
                     TCSGAGVTDTSCTVAHAKGTAGKTFAVTMTCTQ"
     gene            9370..9837
                     /locus_tag="PLH85_12220"
     CDS             9370..9837
                     /locus_tag="PLH85_12220"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB65377.1"
                     /translation="MRKSPFAGATAGFTLIELIAVLLLTGILAIAVLPRMADRSAFDS
                     RGFTDTTRATLQHARRVAIAQRRTVCGTYSGGTLAFTQSSTNGAAGCDLPLPDPAGNA
                     ALSLTPPSGVSLSANPTSFTFDGLGRPNAAVTLTIVGDITRTLTIEADSGYVH"
     gene            9827..10375
                     /locus_tag="PLH85_12225"
     CDS             9827..10375
                     /locus_tag="PLH85_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB65378.1"
                     /translation="MYTSAAGPAPLRGVTLIELIVFIAIVSVGLAGLMSTYTAAVRGS
                     ADPMQRKQALAIAEALLEEVSRAAFTWCDPADANLDTATSAAGCASLPENSGPEAGNS
                     RPYDNVNDYAGFTLAGITDQSGNAISGLSGYSANVAVSAAALNGIAAGEALRVTVTVT
                     APDGNSYALDAWRTRYAPNAPP"
     gene            10353..11171
                     /locus_tag="PLH85_12230"
     CDS             10353..11171
                     /locus_tag="PLH85_12230"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB65379.1"
                     /translation="MHPTRPPEQRSPREAGFTLIEAIVTMTVLAIVGGMIAVFLRTPI
                     DAYTDTARRADLTDTADTALRRFDREIRTALPNSIRSAASGGVLYLEFLQTTGAGRYR
                     IAATSPGVGNPLDFTAADTSFDILGSVPSVPGGAFLVVMNLGAGSGADAYAGDNRSTI
                     ASLSGSTLSFASFLFPFDSPAHRFFVVDTPVTYACNPAAGTLTRISGYTIAASQPTPP
                     ASGTVTLVADKVSACNFTYDAIASRTGMVSASLTLTSGGESVRLFRQVAVSNAP"
     gene            11168..11593
                     /locus_tag="PLH85_12235"
     CDS             11168..11593
                     /locus_tag="PLH85_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65380.1"
                     /translation="MKPRLRARGMALPAAIFLLVIMAALGAFLMRIASLQQAGSAQDI
                     LSSRAFQAARAGVEWALYQAQRNGSCSAATGFSPGGNLADFTVSVTVSTTSYTEAGTA
                     LRLCAVTATACNQPVGGACPGSAGTRYYVERQLRASFPY"
     gene            11750..12664
                     /locus_tag="PLH85_12240"
     CDS             11750..12664
                     /locus_tag="PLH85_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65381.1"
                     /translation="MLKRFFSARHTGWIAAAHQGDALDLVHVSRDGSGRPQLHQAVRQ
                     AGVEGDLAARIASATRASGLRKGRHTWLLHGLDYQILQVDTPDVPEAEQRNALRWQLK
                     DALQQRVEDSVFDLLPLPPPAHARGRPPALVIAAARAALAPVVQAFSAARLDLAAIDV
                     PELALRNIAALHNTAPRGIVLLHFDADGGLLVVCHEGELVTSRRLDVSADQLALADDL
                     RVSRYERLGLELQRSLDNFDRQYSHIPLERLVLSVPPEAASIIDFLASNLYIPVEPLD
                     LGALMGGTGQLGACSLLTLGAALRSEPA"
     gene            12661..13374
                     /locus_tag="PLH85_12245"
     CDS             12661..13374
                     /locus_tag="PLH85_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65382.1"
                     /translation="MSREINLYDASLRPVRDPYTLPALLLAAGAALALIVGGHLLARV
                     QLDDARRAAVDSEARMSALRDRLVEDSRAIAGHVADTALQQRLDEREAELQRLRLADA
                     QIREVLKDQQAPFAEVLRALARRDTDGIWLDSVEIGPGARLTRLQGQATHEALIPDYL
                     ARLGQEVPLAGLAFAQLAIDMPAEAPAAGKPLADPAAAARAAAGKPAAPPPPPAERPR
                     RFTLSARLAATAPTREAPR"
     gene            13371..14042
                     /locus_tag="PLH85_12250"
     CDS             13371..14042
                     /locus_tag="PLH85_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65383.1"
                     /translation="MKRQWTQACARFAALARRERLLVSSAILVGILMLGYTYAIEPQL
                     IQRDKYRQSVALHEDETRRIGEQAALLARQAADPERAKRDALARAEAEGATLRGQVHD
                     LDRLLVTPAAMEAVLTDLLHRRPGVRVLSLVTLPPQPAVARPAGEGDANAKDAAPQGL
                     FRHTLRLQVEGAYAELTALLAELDKGPTRLFRERAVLTADGAGRCRLELELFTLGLNP
                     SWLAL"
     gene            14027..14449
                     /locus_tag="PLH85_12255"
     CDS             14027..14449
                     /locus_tag="PLH85_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65384.1"
                     /translation="MARPVTAPLRRLAALAAILAAPLAVAQALPDPTRPPAGWGPGES
                     PAIAAPAGAPRLQMIVRSPGEAPWALIDGQRVRIGERVDGARLIRLTDTEAVLDDSGT
                     TRRLSLSPGLIRPVAPQAAPAVPSSAASRNAPSQETRR"
     gene            14446..16137
                     /gene="mshL"
                     /locus_tag="PLH85_12260"
     CDS             14446..16137
                     /gene="mshL"
                     /locus_tag="PLH85_12260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HNB65385.1"
                     /translation="MKLPALAVALVAGAAVLSGCSLHTPTKATYSQIEQEVQAAAESR
                     ARSVEPAPAAAPVAPPPRREPDKRFDVAFSNAPAAQVFAALGGSSRYNLLVHPEVAGT
                     ISAHLKNVTLFEALDALRDLYGYDFRVQGNRVTVLPATLQSRIFQINYLAGRRQGQTD
                     VRVTSGSIASSPSANTTGVPSAVPNPTVPNTPGATGAQARAPDSSRVQTSSDQDFWGD
                     ISRSLQIIVGSEAGRSVVINSGSGVILVRAMPGELQQVEQFLRATQLVIERQVMLEAK
                     IVEVQLSEGFQSGINWANLEKGYGVGLDPREINIRSGVSTSTTLGSLLGSGLSSGTGR
                     TDAGIFGIAVKTGNFAALLQFLETQGNVQVLSSPRIATLNNQKAVLKVGTDEFFVTNV
                     TTTTNSSSATTTTSPTITTAPFFSGIALDVTPQIDVDGNIILHIHPSISAVTEKAKNI
                     NLGQLGSITLPLASSNVNETDSIVRIRDGQIVAIGGLMKQTQSDSRSAVPVGGDIPVI
                     GFLLGQRNKSFVKSELVIMLKPTVIRSGDDWRPDLEGFHLRMQDYVPPERDVLRP"
     gene            complement(16272..17615)
                     /locus_tag="PLH85_12265"
     CDS             complement(16272..17615)
                     /locus_tag="PLH85_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNB65386.1"
                     /translation="MRCGMSSCRVRPLAGLLALFALGRAAHAAEGDYETWTARVQGTY
                     VTQYKPSFPAAYSGRNSLSADYEHSYTATMTAYMGFRPWRGGELYLDPELTEGVPFSG
                     LNGSAGFTNGELTRTAGKKPKLYRQRLFLRQTWGLGGGEEAVEADLNQMAGAVDKNRV
                     VLTVGNFSTLDVFDDNAYAKDPRVHFMNAAFMAPLAYDYAADARGFGWGFALEWYQDV
                     WALRIGRMTGPRDPNMLPLDFHLASHYGDQVEVEHAHTWSGQPGKVRVLAFRNRARIA
                     RFDDALAYLRANPAADPQTIFAVRDRDQVKYGVGVNLEQAINADVGVFLRAMRADGQT
                     ETLAFTETDTSVAGGVSVKGRAWGRGEDTVGVGLSRNMLSSARRAYLAAGGLSFFIGD
                     GALNYRPEDLAEAYYSLRLAKGVFLTADMQRMSHPAYNADRGPANFYALRLHGEF"
BASE COUNT         2836 a         7260 c         5022 g         2497 t
ORIGIN      
        1 gcacttgcat ggggtgcaag gggtcgcgag ttcgaatccc gccgccccga ccattgattc
       61 aagcaaaaag ccaacctttc ggggttggct ttttgctttg tggccggctc caccttcttc
      121 ccacagccgt cgttcgcagc gccgtgtctg ctggcagctc actccaaacg ccacccgggt
      181 cctgcaatcg acgtcgggga cgagggcaca gcgcgatgta cgtccctgca ggtccgagcg
      241 gtaccggtca gccttgttgc gcgattgcgt cggcaatcaa ccggtaatac cagaacgact
      301 cgtccgcggc catccgttct ccaaaccggg ccggcgccac gccagtgacc cgcttgatgt
      361 cccggcccat atgggattgg tcggcgtaac cggacgctgc ggcgatcatc gccagattgg
      421 gtgccggccc ggcggcgatg cgccgcacaa acgcctcctc gatgcggaca aagacctcca
      481 ggtcgcgctg gctctggccc gtccaggcgc ggatgcggcg ctgccattgg cgcagactgc
      541 accccgcccc ggagtgcgtg gcgcgcgtcg ccagaccccg cacccagtcc cccagatacg
      601 ggatcgggct gccctgggcc gggagattcc agagcgcccg cagcgtcgtc tccagtttcg
      661 cgaacggggc gtccggcgcc ccgaataccg cgttgcaggc gtcaagcagg gcgggcggcg
      721 cgacgtccgc caagggaacg gtccggttca gccaggcggc cacgggccga ccgagcagac
      781 gcgccatggc ctccgggtag atccccacca tcagcgcatg caccgagccc ggcgaccagc
      841 tcgccgtcgg ccgcgtatgc ggaccggcaa ggatcaggcg cggaagggcc ggccccagac
      901 ccgtcaaggt cgaagcaccg ccagcgcccc cgtcgtcctc catcagatgc agggcgccct
      961 cgaaaatcca cgagatcgac gccatgggac tcgccggata gaagttgaag cgctcccggt
     1021 cagtcagcgt gcagccacgg gtatcgcgct ccaccccggc catcacgcag gcccccaaca
     1081 cgggcgaagg gacatgcagg gtcgcggagg gtcggcgcat gggatgtcgt ttccgttcta
     1141 gcgtgacagg gacggggacc gtcagcatgg cggctgtcat gttccggacg cccatcatgc
     1201 ccaagaccca ttttcacggc cgtttatccc acagactgcg cagtcttgcc acggccgcat
     1261 gcatcgccac ctcccttgcc gcacgccggc tctgcggtcg ccgccgggtt gcatcctggt
     1321 ccttcctgtt cgagtacggc acgctgtaca tccgcgccca gttcaaccat gcctttcgcc
     1381 tgaaggacga catcgccgcc agtcgcgcct atttcgacag cctctattcc gtgttcgaga
     1441 cctatcccga cgtcaccgtc cgcgagaccg ggccaggcga accacgaggc cactggttcc
     1501 tgcccgacaa ccgccggcac gacatcacgc tgctgcactt ccacgggggc ggttatacct
     1561 tctacgccgg cgtgtcccat cacttcgccc gcatcctggc gcacagcctg ggggtggcag
     1621 tcttcgcccc ggattaccgt ctgaccccgg agcaccccca ccccgcccag ctggaagacg
     1681 gactggccgc ctaccgccat ctccttgcca cgggtcacac gccggagcgc atcgtgctct
     1741 gcggcgattc cgcaggcggt cacctggcgc tgatgaccct cgcccatctc gccggcgccg
     1801 gcctgccccg gccccgcctc acgctgggaa tcagtccctg gaccgacatt ggccgacggg
     1861 gcgcgagcca gttcggcaac gacccctacg acctcgttca aggctacatg accctgcagt
     1921 ttgcagactg gctcaagggc ggacaggact ggcgcacgga cgacctctca ccgatccatc
     1981 acgactatcg cggcctcggg cccctctatc tgcaggcggg cgggcgcgag atcctcgtcg
     2041 acatgatccg cgacttcgcc gcgagcgtgg ccggccgggg cggccaggtg cgcctggacg
     2101 tgtggccgga catgaatcat gaattccacg gctacgggga ccatctcccc gaaagcaggg
     2161 aggccctggc gcgcctcgag cgggcgattg catgggcact gcagcccgac acgcccttcg
     2221 tcgccgatac cctcaccgag acggacacct tgacctcatg acaccccggg cgggcaggct
     2281 tccgcgcccc aggatgtccg aacgctggcg ccgacatcgc gtcatgggcg gggcgcccgc
     2341 cctcaagtcc aacgcccccc gtccgtaaat cctgagcgtc ctgcccgttg ccggttcgac
     2401 gttctcccat cgttacggat ccgcccgacg ccatgccaac ttcccgccac cgtacggttg
     2461 ccctgtccgc cctctggcgc cttggcgcgc ggtgcctgct cctgctcgca ttgggcttca
     2521 ccgcccagtc cgtgcgggcc cagacctgct ccctgccggc caactggccg agcggcctgc
     2581 cgggcagttg ccagtgcgac agcttcaacc gcgccagcct caatccgtcc accatcttta
     2641 gcagcaactg gatcgccacc acgagcgaca ctaccggtct gctgccgcgg atcgtcaatt
     2701 ccggctacct gcgcctcacc gacaacaccg gccagaacgc caaatccgcc accgtgccgg
     2761 ccgcctatcc ggccgcaggc aactacatct cggtggaatt ccgccactac gcctacaacg
     2821 gctccggcgc ggacggcatg acgatggtgc tgtcggacta cgccgtcccc gcccagccgg
     2881 gcgccccggg cgggtctctg ggctacgcgc agaatgcgtc ccaggtgggc ttcgccggcg
     2941 gctggatcgg catcgccttc gacgaattcg gcaactacca gaaccccacc gaaaaccgcg
     3001 tcggcggtcc cggcttcatc gccgaatcgg tcggcgtgcg gggctccggt tccggctcca
     3061 gcggctacgc ctggctcacc ggcaccaaca gcctcaccgg cagcaacacc atcgacaacc
     3121 gcacgtccac ctccccgtcc cgcggttacc tgtatcaggt catcgtcgat gcccgcaact
     3181 acacctccag caaccgcacc gccctcttcg cgatcaatcg ggacaccacc gccaccggca
     3241 actcctacgc cagcttcgtg gcccagttcg acgtcttcgc ccgcgcatcc agcctgggcg
     3301 tcagccagtc caatgtgccc accaactggc agatctcctt caccggctcc accggcggct
     3361 ccaccaacat ccatgagatc ggcgccctca agatctgcgc ccagagtgcc gacccgccca
     3421 ccggtggcgc cctgggcggc ttcaatgcca tcgactccac ctacgtccgc ggcaacgtga
     3481 atgcgctgca gggccacctg ttcaccaagc tggccggcac ggcgttccag ctggatattg
     3541 cagccctcaa cagcagcagc tcgggcatat cgtccaacta cgtcctgacc ggtaccaaga
     3601 ccgtcgccat cagcctcatc gacgacagcg ccggcacctc ctgcaacgcc tcggccagcg
     3661 cctgcacggc ctgcagcaag ccggtcatcg ccacccagaa cctggtcttc aattcatccg
     3721 accagggcta caagaccagc ggcaacttca ccgtcaacgg cgcctattca cgggtgatcg
     3781 ccaagatgac cgacggcacc gccaccggct gctccaccga cgccttcagc atccgcccca
     3841 cctccgtttc ggcggtcagc gccaccgcca gcaatgccgc cctcaccggc acccctgtct
     3901 tcaaggccgg caccgacgcc ttcacgatga ccgccacggt ccccgtcacc ggctacaccg
     3961 gtaccctcaa gctcaacacc acctacctcc aggccctgcc caccggcgcc acggccggtg
     4021 ccctcaatcc cctcagcttc accgccgcca ccagcggcgc gggcagctcc accgccacca
     4081 ccagcttcac ctacagcgaa gccggcgcct tcaagctgct gggcaccggc agcaaccacg
     4141 ccctctacga cgacagctgg accaccgtgg accagggctc cgccggcgac tgcgtggccg
     4201 gcagctacag caacacggtg gacagcagcg gcaaattcgg ctgcctgttc ggcatcaccg
     4261 ccgacaccgc gacgatgggc cgcttcaccg ccagcaagtt cgccgtgacg agcagcgccc
     4321 tcaccgccgc gtgcaccgcc agcggcaaga ccgccttcac ctacatgggt cagcccttcg
     4381 ccagcctggc cgtcaccgtg caggcccaga acggcgccgg catcgtgctc gccaactacg
     4441 attcggccct gctttccagc ctcgccggcg tctcgtgggt ggcggaggat tcaaacagcg
     4501 gcaccaatct gggcagccgc atgagcgtcc cggcgcccag caaaacctgg aacgccggcc
     4561 agtacgtggt cagcacgagc gccgccagct tcgcccgcgc ggcatcgcct gacggcagct
     4621 tcgaaaacct gcagctgggc gtcgccgtca gcgacccgga cggcgccctg ctcagcaacg
     4681 ccgatttcaa tcccgccggc accggcgcct gctccggcac cggctgcacc gccaaggccg
     4741 tgggcggcac caccgccctg cgcttcggcc gcctcgccct caccaacgcc tacggctggg
     4801 agaaactgcg cctgccggtg gcgctgcagg cccagtactg gaacggcagc gacttcatcc
     4861 tcaacggcga cgactcctgc acccagttcg cggccaccga cttcgtcctg tccggtttca
     4921 ccggcctggc cgcctgcgac accgccttca ccgccaacaa cagcgccaga ctcaccagcg
     4981 gcgccggtca gctcgtcttc gccgcgccca acaagactgg cagcctcacc gtctccccca
     5041 ccctgggcag cgtcgccagc ggcaccacct gcaccggcag cctgcccacc tccgcggccg
     5101 ctgccacggc ggcgtccaag acctggctcc agggccgctg gaagggcagc gccacctaca
     5161 ccgccaatcc cgccgccaag gtcgccttcg gcctgcaccg gggctccgac aagatcatcc
     5221 agatccgcga attctattga tgcagcatcg gaagcccgcc ctcgccctca agtcgaacgg
     5281 tcccccgccg taattccggg aaatcgaccg ccccttccgg taacgcacgc ccagcccagg
     5341 ccatgaacag accgcagcgc atccgtctcg gcgaagtcct cctgcaacag aacctcctca
     5401 cccaggcgca actcgacacc gccctggaag agcagcgccg cagcggccgc aagctgggcc
     5461 gcatcttcgt ggagagcggc tacgtcaccg aggaatccct cggcaccgcc ctcgcccgcc
     5521 agctcaacat cgccttcatc gacctgcgca gccacagccc gcaggccgag accgtccagc
     5581 tgctgtccga agccctcgcc cgacgcttcc gcgccatcgt gctggacgac accgggcgcg
     5641 ccctgcgcat cggcatgacc gatcccacgg acctcttcgc ctacgacgaa ctggcccgcg
     5701 tcctcaagcg cgaactggag ctcgccgtcg tcggcgaaac cgccctcctc gccgcgctgg
     5761 accgcctcta ccaccggggc gaagagatca acggcctggc caaggccctc accgccgaac
     5821 tcaccgaaga cgccagcgac ttcgccgaac tcttcggctc tggcggcggc caggaagacg
     5881 cgccggtggt gcgcctgctc cagaccgtct tcgaggaagc cgtgcgcctg cgcgcctccg
     5941 acatccacgt ggagccccag gagcgcaccc tgcgcatccg cttccgcatc gacggcgtgc
     6001 tctacgtgca gaccgaggcc gacgtgaaga tcgccccgtc cgtcgccctg cgcctcaagc
     6061 tcatgtccgg cctggacatc tccgaaaagc gcctgcccca ggacggccgc ttcaacatga
     6121 ccatccgcgg cgcaccggtg gacgtgcgca tctcgacact gcccagccag tacggcgaat
     6181 ccgtggtgat gcgtctgctc aaccaggccg ccggcctcgt cagcctcgac cgcatcggcc
     6241 tgcccgacga catgctcgta cgcctgcgcc gcgccgccgc ccgccccagc ggcatgatcc
     6301 tcgtcaccgg ccccaccggc agcggcaaga ccaccaccct ctattccgtg ctcgccgaac
     6361 tcaacagcac cgagcgcaag atcatcaccg tggaagaccc ggtggaatac cgcctgcccg
     6421 gtatcaacca ggtgcaggtg caggacaaga tcgacctgtc cttcgagcgc gtcctgcggg
     6481 ccgccctgcg ccaggacccg gacgtcatcc tcgtcggcga aatgcgcgac aaggccaccg
     6541 ccgagatcgg catgcgcgcc tccatgaccg gacacctggt gctgtccacg ctgcacacca
     6601 acgacgccct gtccacgccc atccgcctgc tcgacatggg cgtcccgcgc ttcatggtcg
     6661 ccatgtccgt gcagctcgtc atcgcccagc gcctgatccg caagctctgc accaattgcg
     6721 ccgaaccgct gcccctgcag ccccatgagc gcgcctggct gcgcctcgag ctgggcagcg
     6781 aggtggacgc ccacagcttc cgccacggcc gcggctgcca cctgtgcaac aaccagggct
     6841 acagcgggcg catgggcatc tacgaatttc tcgaaatgac gccggccctt gtggacgccg
     6901 ccagccacga ggacaaccgc gccttcctcg aagccggccg cgcccagatc cgcggacgca
     6961 ccctgcgcgc cggcgccatg cgcctcgccg cccagggcat cacctcggtg gccgaagcca
     7021 tgcgcgtggc caatgcccag gtggaagacg aaccggacgt gcccgaactg gaacccgtga
     7081 cccgcgatgc ctgagttcgc ctacaccgcc cgcgacggca gcggcgccgc ccttgccggc
     7141 cgcctcgaag ccggctccgc cgaggccgcc gccgagcagc tcctcgcccg cggcgtcacc
     7201 cccgtgtcgg tcacccccgc ccgggccgac gccgccgagc tgctcaatca gtggctcggt
     7261 cgcctgggca gcaccacggt gcccaccgag gcgctgatgc tcttcagtcg ccagatgcac
     7321 accctgctca aatccggcgt ccccatcatg cgcgccctgc gcggcctgca ggaatcggcg
     7381 ccggacgcgc gcctcgccga agccgtcggt gaagtccgcc agtccctcga atccggccgc
     7441 gagctggcct ccgccctggc ccgccatccc cgcgtattct cgcccttcta tgtggccatg
     7501 atccgcatcg gcgaagtcac cggccgcctc gacgagatct tcgtccgcct cttccatcac
     7561 ctggaattcg acaagtacat gcgcgaccag gtgaaggcgg ccctgcgcta cccgagcttt
     7621 gtcatcgccg tgatgatcat cgccctgctg gtggtgaaca tctgggtgat ccccgccttt
     7681 gccaaggttt acgagggctt cgacgcccag ctgcccatcc tgacgcgggt cctggtgggc
     7741 gtgtccgcct tcgtgaagaa ctggtggttc ctgctcatcg gcggcgccgc agccatggcc
     7801 ttcggcttcc gcgcctgggt cgccaccgcc gccggccgcc ttgaatggga ccgcatcaag
     7861 ctgcgcctgc ccatcatggg caagatcatc ctcaaggcca ccctggcccg cttcgcccgc
     7921 agcttcgccc tcgccgtgcg cagtggcgtg cccatcgtgc aggccctcag cgtggtcgcc
     7981 aacaccgtgg acaacgacca catcgcgagc aagatcgaag gcatgcgcga cggcctggaa
     8041 cggggcgaga gcgtcctcaa gggcgccatc gccgccggca tcttcacccc cgtggtgctc
     8101 cagatgatcg ccgtcggtga ggaatccggc tccctcgacg acctcctgga cgaagtcggc
     8161 gtcatgtacc agcgcgaggt cgaattcgag ctgaagaccc tctccagcca gatcgagccc
     8221 atcctcatcg tcttcctggg catcctcgta ttggtgatgg ccctgggcat cttcctgccc
     8281 atctgggacc tgggccgcgt cgccatgaag gggaacggtt gaccatgcgc cccacgctcc
     8341 gccgccggac cggcatcacc accatcgagt tcgccgcctg cctggccctg ctggcagtct
     8401 tcgccgcctt cctgctcaac cgcctcgccg cctacgaaga gctcgccgaa aagaccaccg
     8461 tggacctcac catcagccag atccgcgacg gcatccgcca cgacctgatc cgcgcgctgc
     8521 tggccggcaa gcgccccgtg gtgaatgaga tgctggaacg caaccccgtc tcctggctcg
     8581 cccagccgcc cgaagcctac atgggcgaat tcggctcact ccccggccag gcccggcctg
     8641 gcgcctggta ctacgtcagc aagacccacg agctcgtgta tgtcccgcgc cacagcgccc
     8701 acctcaacac ccccagcagc gacccggtgc gcctgcgctg gcgtctgctg cccgcagcca
     8761 atggcagcgg catggacgcg ctggagcccg tgcgcatcga cgcccagccc gcctacctgt
     8821 ggttctgaac cacaacgatc aattttgcaa ttccggccct aaacctcgtc gaacccctgc
     8881 cgttaccgca ggcataccgc caaacgtccc gaacctcatt ccatccaaga gaggaaccca
     8941 tgaaacagca aagcggcttt accctgatcg aactggtcgt ggtcatcgtc atcctcggca
     9001 tcctggcagc tgtcgccgtg cccaagttcg tggacctgtc caccgacgcg ggcgacgccg
     9061 ccgccgccgg cgtggccggt gcaatcacct ccgcctcctc cctcaactac gccacctcgg
     9121 tcgcccgcgg agccagcggc acgggcgtcc tgcaggtcaa gagcggcggc gtgtgctcga
     9181 ccctggccgg cagtctgctg accaatggcg ttccgaccaa catcaccatc gccggtggcc
     9241 cgctgacctg ctccggcgcg ggcgtcaccg acaccagctg cacggtcgcg cacgccaagg
     9301 gcaccgctgg caagaccttt gcggtcacca tgacctgcac ccagtaagac cccggaggcc
     9361 gtggcatgca tgcgtaagtc ccccttcgct ggcgccacgg ccggcttcac cctcatcgaa
     9421 ctgatcgccg tcctgctcct gaccgggatc ctggccatcg ccgtcctccc ccgcatggcc
     9481 gaccgcagcg cctttgacag ccgcggcttc accgacacca cccgcgccac cctgcagcac
     9541 gcccgccgcg tcgccatcgc ccagcgccgc accgtctgcg gcacctacag cggcggcacc
     9601 ctcgccttca cccagagcag caccaacggc gccgccggct gcgacctgcc gctgcccgac
     9661 cccgccggca acgcggccct cagcctgacc ccgccgagcg gcgtcagcct cagcgcgaac
     9721 ccgacgagct tcaccttcga cggactcggc cggcccaacg ccgccgtcac cctcaccatc
     9781 gtcggcgaca tcacccggac cctcaccatc gaggcggaca gcggctatgt acactagcgc
     9841 cgcaggtccc gcccccctgc gcggcgtcac gctcatcgag ttgatcgtgt tcatcgccat
     9901 cgtctcggtc ggcctcgccg gcctcatgtc cacctacacc gccgccgtgc gcggctccgc
     9961 cgacccgatg cagcgcaagc aggccctcgc catcgccgaa gccctgctgg aggaagtcag
    10021 ccgcgccgcc ttcacctggt gcgacccggc cgatgccaac ctggacaccg ccacctccgc
    10081 cgccggctgc gccagcctgc ccgagaactc cgggccggag gccggcaaca gccgccccta
    10141 cgacaacgtc aacgactacg ccggcttcac cctggccggc atcaccgacc agtcgggcaa
    10201 cgccatctcc ggcctgtccg gctacagcgc caatgtcgcc gtcagcgcgg cggccctcaa
    10261 cggcatcgcc gccggcgagg ccctgcgcgt caccgtgacg gtcaccgcac ccgacggcaa
    10321 cagctacgcc ctcgacgcct ggcggacccg ctatgcaccc aacgcgcccc cctgagcagc
    10381 ggtccccgcg cgaggccggc ttcaccctca tcgaggccat cgtcacgatg accgtgctcg
    10441 ccatcgtcgg tggcatgatc gccgtgttcc tgcgcacgcc catcgacgcc tacaccgaca
    10501 ccgcccgccg cgccgacctc accgacaccg ccgacaccgc cctgcgccgc ttcgaccggg
    10561 agatccgcac cgcactgccc aacagcatcc gcagcgccgc cagcggcggc gtgctctacc
    10621 tggaattcct ccagaccacc ggcgccgggc gctaccgcat cgccgccaca agccccggcg
    10681 tcggcaaccc ccttgacttc accgccgccg acaccagctt cgacatcctc ggcagcgtcc
    10741 ccagcgtgcc cggcggcgcc ttcctggtgg tcatgaacct gggcgcaggc agcggcgcag
    10801 acgcctatgc gggcgacaac cgcagcacca tcgccagtct ctccggcagc accctcagct
    10861 tcgcatcctt cctctttccc ttcgactcgc ccgcccaccg cttcttcgtg gtggatacac
    10921 cggtcaccta cgcctgcaac cccgccgccg gcaccctcac ccgcatcagc ggctacacca
    10981 tcgcggccag ccagcccacg ccgcccgcca gcggcaccgt caccctggtg gccgacaagg
    11041 tcagcgcctg caacttcacg tatgacgcca tcgccagccg caccggcatg gtctccgcct
    11101 ccctcaccct cacctccggc ggcgagtccg tacgcctctt ccgtcaggtc gccgtgagca
    11161 acgccccatg aaaccccgcc tgcgcgcccg cggcatggcc ctgcccgccg ccatcttcct
    11221 gctggtcatc atggccgccc tgggcgcctt cctgatgcgc atcgccagcc tgcagcaggc
    11281 cggctcggcc caggacatcc tctcctcccg cgccttccag gccgcccgcg ccggtgtgga
    11341 gtgggctctc taccaggccc agcgcaacgg aagctgcagc gccgccaccg gcttctcgcc
    11401 ggggggcaat ctggccgact tcaccgtcag cgtcaccgtc agcaccacca gctacaccga
    11461 ggccggcacc gccctgcgcc tgtgcgccgt caccgccacc gcctgcaatc agccggtcgg
    11521 tggcgcctgc cccggcagcg ccggcacccg ctactacgtc gagcgccagc tgcgtgcaag
    11581 ctttccctac tgacccgagc gccccacaca cgataagggc cggatttacg gtgcttttct
    11641 caagctcagg cgcctaaagt ccttgcggcg gcagccgtta agctgcgtgg taagccttgc
    11701 cgccccgtgc cgggaaccgg cttggatgcg tcctccgggc ctccccagaa tgctgaaacg
    11761 atttttctcc gcgcgacaca ctggctggat cgccgccgcc caccagggcg acgcgctgga
    11821 tctcgtccat gtcagccgtg acggcagcgg gcgcccgcag ctccaccagg cagtgcgcca
    11881 ggccggcgtc gaaggcgatc tcgccgcgcg catcgcctcc gccacccgcg ccagcggcct
    11941 gcgcaagggg cgccacacct ggctgctcca cggcctcgac taccagatcc tccaggtgga
    12001 cacccccgac gtacccgagg ccgaacagcg caacgccctg cgctggcagc tcaaggacgc
    12061 cctgcagcag cgtgtggagg acagcgtctt cgacctgctg ccgctgccgc ccccggccca
    12121 cgcccgcggc cgtccgcccg ccctcgtgat cgccgccgcc cgcgcggcgc tggcgcccgt
    12181 ggtgcaggcc ttcagcgccg cccgcctcga tctggcggcc atcgacgtgc ccgaactggc
    12241 cctgcgcaac atcgccgccc tccacaacac cgcgccgcgg ggcatcgtgc tcctgcactt
    12301 cgacgccgat ggcggcctgc tggtggtctg ccacgaaggc gaactcgtca cctcgcgccg
    12361 cctcgacgtc tccgccgacc agctcgccct cgccgacgac ctgcgcgtct cccgctatga
    12421 acgcctgggc ctggagctgc aacgctccct cgacaacttc gaccgccagt acagccacat
    12481 tcccctcgaa cgcctggtcc tctcggtgcc ccccgaagcc gccagcatca tcgacttcct
    12541 ggcctccaac ctgtacatcc ccgtcgaacc cctcgacctc ggcgcgctga tgggcggcac
    12601 cgggcagctc ggtgcctgct ccctcctcac cctcggcgcg gccctgcgct cggagcccgc
    12661 atgagccgcg agatcaacct ctacgacgcg agcctgcgcc ccgtccgcga tccctacacc
    12721 ctgcccgccc tcctgctggc ggccggggcc gccctcgccc tgatcgtcgg cggccatctc
    12781 ctcgcccgcg tccagctcga cgacgcccgc cgcgccgccg tcgacagcga agcccgcatg
    12841 agcgccctgc gcgaccgcct ggtggaggat tcccgcgcca tcgccggcca cgtggccgac
    12901 accgccctgc aacagcgcct cgacgaacgg gaggccgaac tccagcgcct gcgcctcgcc
    12961 gacgcacaga tccgcgaagt cctcaaggac cagcaggccc ccttcgccga ggtgctgcgc
    13021 gccctcgccc gccgcgacac cgacggcatc tggctcgaca gcgtcgagat cggccccggc
    13081 gcgcgcctca cccggctgca gggccaggcc acccacgaag ccctgatccc cgactacctc
    13141 gcccgcctcg gccaggaagt ccccctcgcc ggcctggcct ttgcccagct ggccatcgac
    13201 atgcccgccg aagcccccgc cgccggcaag cccctcgccg atccggccgc ggcggcccga
    13261 gcggcggccg gcaaacccgc cgcaccgcct ccgccgcccg ccgaacgccc ccgccgcttc
    13321 accctgtccg cccgactggc cgccacggcc cccacccggg aggcgccgcg atgaagcgcc
    13381 aatggaccca agcctgcgcc cgcttcgccg ccctcgcccg gcgtgagcgc ctgctggtga
    13441 gcagcgccat cctggtcggc atcctcatgc tcggctacac ctacgccatc gagccccagc
    13501 tcatccagcg cgacaagtac cgccagagcg tcgccctgca cgaagacgaa acccgccgca
    13561 tcggcgaaca ggccgccctc ctcgcccgcc aggccgccga tcccgagcgg gccaagcgag
    13621 acgccctggc ccgcgccgaa gccgaaggcg ccaccctgcg cggccaggtc cacgacctgg
    13681 accgcctgct ggtcaccccc gccgccatgg aggcggtgct caccgacctg ctccaccgcc
    13741 gccccggcgt gcgcgtgctg tccctcgtca ccctgccgcc ccagcccgcc gtggcccgcc
    13801 cggcgggcga gggcgacgcc aacgccaaag acgccgcgcc gcaaggcctc ttccgccaca
    13861 ccctgcgcct gcaggtggaa ggcgcctacg ccgaactcac cgccctgctg gccgagctgg
    13921 acaagggccc gacccgcctc ttccgcgaac gggccgtgct caccgccgac ggcgccggcc
    13981 ggtgccgcct cgaactggaa ctgttcaccc tcggactgaa cccgtcatgg ctcgccctgt
    14041 gaccgcccca ttgcgccggc tcgcggccct cgcggcgatc ctcgccgccc ccctcgccgt
    14101 ggcccaggcc ctgcccgacc ccacccgtcc gcctgccggc tggggccccg gcgaatcgcc
    14161 cgccatcgcc gcaccggcgg gcgccccccg cctgcagatg atcgtgcgca gccccggcga
    14221 agccccctgg gccctcatcg acggccagcg cgtacggatc ggcgagcgcg tcgatggcgc
    14281 ccgcctcatc cgcctcaccg acaccgaagc cgtcctcgac gacagcggca cgacccgccg
    14341 cctgagcctc tcgccgggcc tcatccgccc cgtcgcgccc caggcagccc ccgccgtgcc
    14401 gtcctccgct gcatcccgta acgccccgtc ccaggagacc cggcgatgaa acttcccgcc
    14461 ctcgccgtcg ccctcgtcgc cggtgccgcc gtcctctccg gctgctccct gcacacgccc
    14521 accaaggcca cctacagcca gatcgagcag gaggtccagg ccgccgccga atcccgcgcc
    14581 cgcagcgtcg agcccgcccc ggccgccgcc ccggtcgcgc cgccgccccg ccgcgaaccc
    14641 gacaagcgct tcgacgtcgc cttcagcaac gcccccgccg cccaggtctt cgccgccctc
    14701 ggcggcagct cccgctacaa cctgctggtg caccccgaag tcgccggcac catcagcgcc
    14761 cacctcaaga acgtcaccct cttcgaagcc ctcgacgccc tgcgcgacct ctacggctac
    14821 gacttccgcg tgcagggcaa ccgggtcacc gtgctgcccg ccaccctcca gtcgcgcatc
    14881 ttccagatca actacctggc cggccgccgc cagggccaga ccgacgtgcg cgtcacctcc
    14941 ggctccatcg ccagcagccc cagcgccaac accaccggcg tgcccagcgc ggtgcccaac
    15001 cccaccgtgc ccaacacccc cggcgccacc ggcgcccagg cccgcgcgcc cgacagcagc
    15061 cgcgtgcaga cctcctccga ccaggacttc tggggcgaca tcagccgctc cctgcagatc
    15121 atcgtcggca gcgaggccgg ccgcagcgtc gtcatcaaca gcggctccgg cgtcatcctg
    15181 gtgcgcgcca tgcccggcga actgcagcag gtggagcagt tcctgcgcgc cacccagctg
    15241 gtcatcgaac gccaggtcat gctcgaagcc aagatcgtcg aagtgcagct ctccgaaggc
    15301 ttccagtccg gcatcaactg ggccaacctg gaaaaaggct acggcgtcgg cctcgacccg
    15361 cgggagatca acatccgctc cggcgtgagc accagcacca ccctgggcag cctgctcggc
    15421 agcggcctca gcagcggcac cgggcgcacc gacgccggca tcttcggcat cgccgtcaag
    15481 accggcaact tcgccgccct gctccagttc ctcgaaaccc agggcaacgt gcaggtgctc
    15541 tccagcccgc gcatcgccac cctcaacaac cagaaggccg tgctcaaggt gggcaccgac
    15601 gaattcttcg tcaccaacgt caccaccacc accaacagca gcagcgccac caccaccacc
    15661 tcgcccacca tcaccacggc gcccttcttc tccggcatcg ccctcgacgt cacaccccag
    15721 atcgacgtgg acggcaacat catcctgcac atccacccgt ccatcagcgc cgtcaccgaa
    15781 aaggccaaga acatcaacct cggccagctc ggctccatca ccctgccgct ggcctccagc
    15841 aacgtgaacg agaccgactc catcgtgcgc atccgcgacg gccagatcgt cgccatcggc
    15901 ggcctcatga agcagaccca gtccgattcc cgcagcgccg tgccggtggg cggcgacatc
    15961 ccggtgatcg gcttcctcct cggccagcgc aacaagagct tcgtcaagag cgagctggtc
    16021 atcatgctca agcccaccgt catccgctcc ggtgacgact ggcgcccgga cctggaaggc
    16081 ttccacctgc gcatgcagga ctacgtcccg ccggaacggg acgtgctgcg cccctgacac
    16141 accgcgcccc cctcaaaaca caaagccccg gtcgagccgg ggctttgtca ttgaaggcgc
    16201 cgccggcacg caccggccgc cgcgggcgac gcgccctacc ggcgcatcgc ccccatcccc
    16261 gccgcccgcc ttcagaactc cccgtgcagg cgtagcgcgt agaaattggc cgggccacgg
    16321 tccgcgttgt aggccgggtg gctcatgcgc tgcatgtccg cggtcaggaa cacccccttc
    16381 gccagacgca ggctgtaata cgcctccgcc agatcctccg gccggtagtt gagcgccccg
    16441 tcgccgatga agaaggacag cccgcccgcc gccagatagg cccggcgcgc gctcgacagc
    16501 atgttgcggg acaggcccac gcccaccgta tcctcgccgc gcccccaggc cctacccttc
    16561 accgacacac cgcccgccac cgacgtatcc gtctcggtga aggccagcgt ctccgtctgc
    16621 ccgtccgccc gcatggcccg caggaagacg cccacgtccg cgttgatcgc ctgctccagg
    16681 ttgaccccca cgccgtactt cacctggtcc cggtcgcgca ccgcaaagat cgtctgcggg
    16741 tccgccgccg ggttggcgcg cagataggcc agcgcatcgt cgaagcgggc gatgcgcgcc
    16801 cggttgcgaa acgccagcac gcgcaccttg cccggctggc cggaccacgt gtgggcgtgc
    16861 tccacctcca cctgatcgcc gtaatggctc gccagatgaa aatccagggg cagcatgttc
    16921 ggatcccgcg ggcccgtcat gcgcccgatg cgcagcgccc acacatcctg ataccactcc
    16981 agcgcaaagc cccagccgaa gccccgcgca tccgccgcgt agtcataggc cagcggcgcc
    17041 atgaaggccg cgttcatgaa atgcacccgc ggatccttgg cgtaggcgtt gtcgtcgaac
    17101 acatccagcg tggaaaagtt gcccaccgtc agcaccaccc ggttcttgtc caccgcgccc
    17161 gccatctggt tcagatccgc ctccaccgcc tcctcacccc cgcccaggcc ccaggtctgg
    17221 cgcaggaaca ggcgctggcg atacagcttg ggcttcttgc ccgccgtgcg cgtcagctcc
    17281 ccgttggtga agccggcgct gccgttgagc cccgagaacg gcaccccctc ggtgagctcc
    17341 gggtccagat acagttcccc cccgcgccac ggccgaaacc ccatgtaagc cgtcatcgtg
    17401 gccgtgtaac tgtgctcgta gtccgccgac agactgttgc gcccgctgta ggccgccgga
    17461 aaggacggct tgtactgggt gacatacgtc ccctgcaccc gcgccgtcca cgtctcgtag
    17521 tcaccctcgg ccgcgtgggc cgcgcgcccc agggcgaaca gcgcgagaag accggcaagg
    17581 ggacggacac gacatgaaga catgccgcag cgcaa
//
LOCUS       DAOQMM010000077        17597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1216_length_17597_cov_52.069490, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000077 DAOQMM010000000
VERSION     DAOQMM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17597
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1216_length_17597_cov_52.069490"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLH85_12270"
     CDS             complement(<1..830)
                     /locus_tag="PLH85_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNB65387.1"
                     /translation="MSVSKKMLSLLLSAALGIVILAALGLTQMSRVYEITNYANVNVV
                     PSIQTLDKAFSPMAASRTQLWQHIAASDPAVMSTLEERISANRKKVDEALKAYEPLIS
                     DDKDKSLLAADRAALADYDQLRERVYVLSRVNKMEEARDLVMANQAIMARLWDAFEEH
                     REYNAELGRKAADEAAQTLGIARWQAITISLVTLVAVCLIGFFITRSLMRQLGGEPAY
                     AAEVVAKIAEGDLSTEVLAKAGDTSSLLYSVKCMSERLAAIIAEVRTTADSLASSSEE
                     V"
     gene            complement(861..3146)
                     /locus_tag="PLH85_12275"
     CDS             complement(861..3146)
                     /locus_tag="PLH85_12275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNB65388.1"
                     /translation="MSIQLDDALQTFIVESRELLQQMEEALLEMEHGTDDPDTLNAIF
                     RAAHTIKGSAGLFGLDDIVAFTHNVESLLDRIRDHRLALTRSIGGLLLESSDHMGALV
                     SCAAAGEAPDLGLRARSEVLGQRLKEVMNGGTATPASANATAPAGSAVAEVDADLAAL
                     DWPDGQDLPAVATSVRSDGVSSGLWHISLRAGPDLLRNGMDPLSILHYLSTLGQIVSL
                     EMVLGALPPLTMIDPESCYLGFELSFASAAGRDSIDGAFDFVREDSIVRVIEPYGSLD
                     DYRALLGALPEDRQLLGEMLVQCGSLTRDELAQALATRDRQAADSAVAGDPAPRLGEV
                     LVQQQLVDAPLVEAAVERQQQLRGQGTAKGGEASLIRVDAAKLDQLINLIGELIIAGA
                     GTQLIARRLKVPELAEASELLSRLVESVRDSALDLRMVQIGATFNRFRRVVRDVAAEL
                     GKDIELDIRGGDTELDKTVVERIGDPLTHIVRNALDHGIEPAAQRVAAGKSAQGRLSL
                     NAYHDSGSIVIEVSDDGGGLRKERILRKAIERGLVAEGQTLSDKEIFHLVFEPGFSTA
                     EQVSNLSGRGVGMDVVKRNIQALRGTVDLESSEGQGTTVRIRLPLTLAIIDGFLIGAG
                     PSSYVVPLDQVVECIELPAGESPEHGASYLNLRGEVLPFIRLRDLFGTGGEAPRRENV
                     VVVQYAGERAGLVVDQLYGEFQTVIKPLGKVFSHIRCIGGSTILGSGEVALIIDVPGL
                     VRQVSTRHSAAKNAPLSMLPA"
     gene            complement(3163..3477)
                     /locus_tag="PLH85_12280"
     CDS             complement(3163..3477)
                     /locus_tag="PLH85_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB65389.1"
                     /translation="MNASEQTILGTAHVVIAGEMTIYTARDWRDRLLIAMEAPGDVVL
                     DLSGVSDIDAAGIQLLASLALEAKGAERVLRFAAVSPRILEAFEFCRLMDFFKDALAG
                     AR"
     gene            complement(3504..3872)
                     /locus_tag="PLH85_12285"
     CDS             complement(3504..3872)
                     /locus_tag="PLH85_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB65390.1"
                     /translation="MAKTILIVDDSASLRQVVSIALRGAGYDVVEGCDGRDGLTKLDG
                     RKIHLIISDVNMPNMDGISFLKALKQVPAYRFTPVIMLTTEAGDDKKREGQAAGAKAW
                     VVKPFQPQQMLTAVAKLILP"
     gene            complement(3882..5051)
                     /locus_tag="PLH85_12290"
     CDS             complement(3882..5051)
                     /locus_tag="PLH85_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65391.1"
                     /translation="MKQAGAQGPVGAMPILWYGVTVLVAGVPTLAAGGRLEVLGASLA
                     VALGVLAMAWRTARPLLPTPEPAYGPAADAPPAAVEGLDRLCEDVLPLWRDQVSMVRS
                     QTEEAITALSVRFGDLSRRVESAAGSASDDGSGNLATILNASEAELSGIVAELRRALE
                     NKDVLLGQVEELARLTSSLQSMARDVGDVAKQTNLLALNAAIEAARAGEAGRGFAVVA
                     DEVRKLSTASGETGKKIGDIIATVTSAMGDALSVSRAYAQSDAQLVSDAGRRIGEVVE
                     RLRGAAGGVVARAREVSAESHAVATEIGDVLVALQFQDRVSQVLGSVSGDMDRLQQRV
                     RSCRAELAAGLHPDPLDPIAWRRDMARQFTTPEQHALQRGANPVTAREDAGITFF"
     gene            complement(5069..5686)
                     /locus_tag="PLH85_12295"
     CDS             complement(5069..5686)
                     /locus_tag="PLH85_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HNB65392.1"
                     /translation="MKTDDPTSLTCSTRDAARRLGISVRTAQLWVEEGRLRAWKTPGG
                     HRRILRESVERLVEEQRLAAFGPGGRFRILVLREDEAGREALGQQLEGMLPDCVVVTA
                     GNGFDALLRIGDEAPEMLISDLGLTGIDFFRMVNTLVEGARTRGMLVIVLVASEADCM
                     GVRARLPDDVVLMRTPVDEAELLALVKAFRGQWRRHRSGATEQGV"
     gene            6301..9177
                     /locus_tag="PLH85_12300"
     CDS             6301..9177
                     /locus_tag="PLH85_12300"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNB65393.1"
                     /translation="MSAIAKDADARTAATLAPQEISGEVLLEKYAKGNEQTVADVRTR
                     VARALAAMENEDKRAHWEARFLEAQERGFVPAGRINSAAGTKLAATLINCFVQPVGDS
                     ITESVDGRCGIYTALAQSAETMRRGGGVGYDFSSIRPRGAMVRGTQSNASGPVSYMRV
                     FDRSCETVESAGSRRGAQMGVLRCDHPDIEDFIHAKDHGDLTNFNVSIAVTDLFMQAV
                     EAGTDVELAHKAEPASDIKAAGAYQREDGLWVYRKVPARDLWDQVMRSTYDHAEPGIL
                     FLDRMNRDNNLYYCETIEATNPCAEQPLPSYGCCCLGSINLTLYVKDPFTAKAAFDFA
                     AFGKTVETSIRMLDNVLEATHWPLEQQHAEAQSKRRVGLGFTGLGNALAMLCLRYDTT
                     EARAMATRISEFMRDQAYLASVELARERGAFPLFNADLYLSGGNFASRLPQDIKDKIR
                     KHGLRNSHLLSIAPTGTISLAFADNASNGIEPPFSYTYTRKKRMADGTFKEYAVEDYA
                     WRLYKHQGGDVNKLPEYFVTALEISAKAHKDMVAAVAPFVDTSISKTVNVPEDYPYAD
                     FEDLYLEAWKAGLKGLATYRPNSVLGSVLSVSTPSTEQKQPQDVEISDTNKRLSIKSL
                     PAPVLSSLRWPGRPDLPDGNAAWTYMLNTPQGNFALFVGHMDPIEDSPAKGFFPFEVW
                     VNGADQPRGLGAVAKTLSMDMRANDRGWLKLKLETLAKTVGEKSFEMRFPPHGEQKLM
                     PGVVSAFAQVVRYRCEKLGALEGDLPNPVLDTLFSLQEPKTGTDGTLSWTVDIQNPAT
                     GEDFVLGLKEITLPDGVTRPYSMWLSGNYPRALDGLSRILSLDMRVMDPAWIGMKLRK
                     LIDYPEPLGDFMAFVPGTRRQQNWPSTVAYMAALIIHRYAMLGVLDEAGYPTREMGIL
                     EAPRDDNEPKLMQGALCNECGNYSVIRKDGCDFCTACGAVGTCG"
     gene            complement(9370..10338)
                     /locus_tag="PLH85_12305"
     CDS             complement(9370..10338)
                     /locus_tag="PLH85_12305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNB65394.1"
                     /translation="MNRLGLGFVACLIAAPVAAESSAVVAFVGDVMLARTEATGRLIA
                     RGGDPFRRVRSILAPADLRVANFESAGGRSGKADPAKPFSFRTTAAGVVAFSSVFEVA
                     GLANNHAVDFGRQALVETLASLRQSGSLGVGAGRDLVEAHRALIVERQGVRIGLLGYL
                     DFFPRWFAAAPGLPGVAWLDPRQVAVDIADARARGAEVVIVIPHWGEEHQPQANEHQR
                     SRARAIIDAGADAVIGGHPHVVQDYEIYRGKPILYSLGNFVFDGFSDEDNITGWAVFA
                     TVDSAGVSAIRTRVVRLDGHGAPAPDASKSGPCWTRGEAQMTRCAE"
     gene            complement(10335..10721)
                     /locus_tag="PLH85_12310"
     CDS             complement(10335..10721)
                     /locus_tag="PLH85_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65395.1"
                     /translation="MSVLRKPEVCFRTALLMALGLLSSAAALAVECTLEQGSMIRRMD
                     VPAGETSLAGQWATLGVFRVRTALTTPRQGRAWLLIEVYAEAGRQDFRILSSHTVESP
                     FDTGRVDVVAPRLGQSLTYTCKDDHP"
     gene            10981..11325
                     /locus_tag="PLH85_12315"
     CDS             10981..11325
                     /locus_tag="PLH85_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65396.1"
                     /translation="MDRIDARPEAFAHLVYRYLWPYAYFRDVTRGSFLERQQNYRHNR
                     RMGMHLPGFMLKWACLTVFFYLLGSLCEELLELVLPAACCYVTGAWTLTILVQLCVAW
                     LWLKRFPDIPRG"
     gene            complement(11365..12369)
                     /locus_tag="PLH85_12320"
     CDS             complement(11365..12369)
                     /locus_tag="PLH85_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNB65397.1"
                     /translation="MMLRTALLALILQLLALPAGAEGPIVIKFSHVVAADTPKGKASE
                     FFKRRAEELTRGRVKVEVYPNSSLYKDKEELEALQLGAVQMLAPSLAKFGPLGGKEFE
                     VFDLPYIFDNYADLHRVTQGPVGASILRQLDGKGIIGLAFWDNGFKSFSANTPIRTPA
                     DLKGKKLRIQPSKVLEEQVRALGALPQVMAFSEVYQALRTGVVDGTENPHSNMYTQRM
                     HEVQKYLCLTDHGYLGYAVIANKKFWDGLPGDVRSQLEQAMKESTVYANRIAQEENER
                     SLELIRKSGKTQIHVPSKEEREAFRRVLIPVHARMEARIGASLIRAIYRETGFDPNRP
                     "
     gene            12565..14541
                     /locus_tag="PLH85_12325"
     CDS             12565..14541
                     /locus_tag="PLH85_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB65398.1"
                     /translation="MSQNPLNFRPPSPPPPSWYWGLPYVAVVLFIATVALLLWLTHRQ
                     DAEEQRGILINDVLWMEQNLSFQFERNAALLTQLGPELLGKIADDSTEARLKQALDPD
                     TGLVRILWLREDGRVKGAEPPFTDTHLVGEASGTFPSDATSRLARSMGRATYSDTYAV
                     IDNQAQFEVHVPVFERGRYVGAVVGVYSLNELLQHQIPWWFSERYRVTVTNNLGDEIA
                     AKSKVAPLSTLAYEIPFEPPGHGLSVSVTAYKSEVRWVPLLLMGSLVILGCAIVWSLW
                     ALRRHMRRRQAAEQALRAEHAFRKSMEDSLNTGMRARDLEGRITYVNPAFCRMVGFSA
                     DELIGMRPPMPYWPPEDLERILDVHAMILSGHAPSQGVEFRLMRRDGTRFDVLLIEAP
                     LIDPNGRHIGWMGSMVDVTEQKRAQELARQQQERLQATARLVTMGEMASTLAHELNQP
                     LAAISSYNTGCLNLIRQGDLPREELTGILEKIGRQAQRAAQIIRRVHDFVRRREPNFV
                     AADFNAILREAVELVEPTAERRGVSIDCDFTMPLPAVSADPVMIEQVAVNLIRNGMDA
                     MADTPIDERRLQLRTALRDGMVCLTVEDRGAGIPADVAEKLFAPFFTTKSEGMGMGLN
                     ICRSIAELHRGRLAYEAQPAGGTVFHFSVPVLQP"
     gene            14538..15152
                     /locus_tag="PLH85_12330"
     CDS             14538..15152
                     /locus_tag="PLH85_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB65399.1"
                     /translation="MNTPLAHIVDDDEAIRDALAWLFRTRAVECRAWESGEAFLDAWQ
                     PDWRGCIVLDIRMHGMNGLECFDALNARGNALPVIFLTGHGDVPMAVGALKAGAFDFL
                     EKPFKDNDLVDVVLRAIDSDTRRHASQATQADIAGRMAQLTPREHEVMQRVLAGKFNK
                     VIADELNISMRTVEVHRSRVFEKMGVRSAVELAQFLAPGREGRD"
     gene            complement(15149..16480)
                     /gene="tilS"
                     /locus_tag="PLH85_12335"
     CDS             complement(15149..16480)
                     /gene="tilS"
                     /locus_tag="PLH85_12335"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNB65400.1"
                     /translation="MPSTELVDQVARSWRRLPAPARKRLRVGLSGGLDSSVLLHVLAS
                     VRRAEGFGLSAVHVHHGLVPGADRWGRHCGVFCANLDVPLRVVDVVVDRESPAGIEAA
                     ARMARRAALLAEGGVVALAHHRDDQAETVLFRAVRGAGVRGLAGMREIQSQSDGLVWR
                     PLLNTYRAELEAYARAVGLVWIEDPSNMDHAHTRNYLRHELMPRLSVRFPGSVGTLAR
                     LGRLAGEASDLLDEMANEDRARLSCSDGSTRLAYAASLHLSSARWRNLLRSLLVDADP
                     ILPDEDRLREAERQFRSVGAPQGLRCRLGTQALCVYRDRWWLEGDSRDEGTPEGLALT
                     SLAALRWAGAALDFRAVTGAGIMRAALIGAPVRIDRRRGGERLSLHPAGPSRTLKNLF
                     QEAGIPPWLRPRLPVLWVGDRVAWVAGVGVAGEFRCPADAPGVVPEWNGLV"
     gene            complement(16467..17435)
                     /locus_tag="PLH85_12340"
     CDS             complement(16467..17435)
                     /locus_tag="PLH85_12340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNB65401.1"
                     /translation="MKTTFLEFEQPIAELEAKIEELRFVQDDSAVDISEEIARLEQKN
                     HGLTKDIYAKLTPWQCALVARHPQRPYTLDYVENIFTDFEELHGDRAFADDKAIVGGL
                     ARFNGQSCVVIGHQKGRDTKEKIFRNFGMPRPEGYRKALRLMRLAEKFGLPVFTFVDT
                     PGAYPGIDAEERGQSEAIGHNLFVMAELKVPIICTVIGEGGSGGALAIAVGDQVLMLQ
                     YSTYSVISPEGCASILWKSAERAADAAETMGITASRLKSLGLVDKVVSEPVGGAHRDH
                     RVMSQSLKRALQDALRQVSELSPTELLERRFERLMSYGQFKEIAVD"
BASE COUNT         3078 a         6024 c         5485 g         3010 t
ORIGIN      
        1 acttcctcgg acgagctggc gagggagtcg gcggtggtgc gcacttcggc gatgatggcg
       61 gcaagccgct cggacatgca cttgacgctg tagagcaggc tgctggtgtc gcccgccttg
      121 gcgagcactt ctgtggacag atcgccttcg gcaatcttgg ccacgacttc cgcggcgtag
      181 gccggctcac cgcccagttg ccgcatcagg ctgcgggtga tgaagaagcc gatcagacac
      241 accgccacca gggtgacgag gctgatggtg atggcctgcc agcgggcgat gccgagggtc
      301 tgggcggcct cgtcggcagc cttcctgccc agttcggcgt tgtactcgcg gtgctcttcg
      361 aaggcatccc agaggcgggc catgatggcc tggttggcca tgaccagatc ccgggcttct
      421 tccatcttgt tgacacggga cagcacatag acgcgctccc gcaactggtc gtagtccgcc
      481 agggcagccc ggtccgcggc gagcaggctc ttgtccttgt cgtcgctgat gagcggctcg
      541 taggccttga gggcttcatc caccttcttg cggttggcgc tgatgcgctc ttcgagggtg
      601 ctcatcaccg ccgggtccga cgcggcgatg tgctgccaca gctgggtacg agacgcggcc
      661 atgggactga aagccttatc cagggtctgg atgctgggca ccacattgac gttggcgtag
      721 ttggtgatct cgtagacccg gctcatctgg gtcaatccga gggcggccag gatgacgata
      781 ccgagcgctg cggacagcag cagggataac atttttttgc tgacggacat ggttggttct
      841 cctgagagag gctgccgggc tcacgccggg agcattgaga ggggggcgtt cttggcggcg
      901 gagtgacggg tgctgacctg gcgcacgagg cccggtacat cgatgatcag ggccacttcg
      961 ccactgccca gaatcgtgga accgccaatg cagcggatgt gactgaagac cttgccgagg
     1021 ggcttgatga cggtctggaa ttcgccgtag agctgatcca ccacgagtcc ggcgcgctcg
     1081 ccggcgtact ggaccaccac cacgttttcg cgccggggcg cttcaccgcc ggtgccgaac
     1141 agatcccgca ggcggatgaa gggcagcacc tcgccgcgca ggttgaggta gctggcgccg
     1201 tgctcgggac tttcgccggc cggcagttcg atgcattcca ccacctggtc gaggggcacc
     1261 acgtaggacg aggggccggc gccgatgagg aagccgtcga tgatggccag ggtgaggggc
     1321 aggcggatgc gcacggtggt gccctgccct tcgctgcttt ccaggtcgac ggtgccgcgc
     1381 agggcctgga tgttgcgctt gaccacatcc atgcccacgc cgcggccgga caggttgctg
     1441 acctgctcgg cggtggaaaa gccgggctcg aagaccaggt ggaagatctc cttgtccgac
     1501 agggtctggc cttcggccac caggccgcgc tcgatggcct tgcgcaggat gcgttccttg
     1561 cgcaggccgc cgccgtcgtc gctgacttcg atgacgatgc tgccggagtc gtggtaggcg
     1621 ttgagggaca ggcgaccctg ggcggatttg ccggcggcga cgcgctgggc ggcgggctcg
     1681 atgccgtggt cgagggcgtt gcgcacgatg tgggtgagcg ggtcgccgat ccgctcgacg
     1741 acggtcttgt ccagctccgt gtcgccgccg cggatgtcca gctcgatgtc cttgcccagc
     1801 tcggcggcca cgtcgcggac gacgcggcgg aagcggttga aggtggcgcc gatctgcacc
     1861 atgcgcaggt cgagggcgga gtcgcgcacg ctttcgacga ggcgcgacag cagctcgctg
     1921 gcctcggcca gttccggcac cttgaggcgg cgggcgatga gctgggtgcc ggcgccggcg
     1981 atgatgagtt cgccgatgag gttgatgagc tggtcgagct tggcggcatc cacgcggatc
     2041 aggctggctt cgccgccctt ggcggtgccc tggccgcgga gctgctgctg gcgttcgacg
     2101 gcggcctcca ccagcggcgc atccacgagc tgctgctgga cgaggacttc gcccaggcgc
     2161 ggggcgggat cgccggcgac ggcgctgtcg gcggcctggc ggtcgcgggt ggcgagggcc
     2221 tgggccagtt cgtcgcgggt gagggagccg cactgcacga gcatctcgcc gaggagctgg
     2281 cggtcttccg gcagggcgcc gagcagggcg cggtagtcgt ccagggaacc gtagggttcg
     2341 atgacgcgga cgatgctgtc ttcgcgcacg aaatcgaagg cgccgtcgat ggaatcccgc
     2401 ccggcggcgc tggcgaagct cagttcgaag cccaggtagc agctctccgg gtcgatcatg
     2461 gtgaggggcg gcaaggcacc gaggaccatc tccaggctga cgatctgccc gagggtggac
     2521 aggtagtgga ggatggagag ggggtccatg ccgttgcgca gcaggtcggg cccggcgcgc
     2581 agggagatgt gccacaggcc gctgctgacg ccgtcgctgc gtacggaggt tgccacggcg
     2641 ggcaggtcct ggccgtcggg ccagtcgagg gcggccaggt cggcatcgac ctcggccact
     2701 gctgaccctg ccggcgctgt cgcattggcg gacgcgggcg tggcggtgcc gccgttcatg
     2761 acctccttga ggcgctggcc gaggacttcg ctgcgggcgc gcaggcccag gtccggcgcc
     2821 tcaccggcgg ccgcgcagct caccagggcg cccatgtgat cgctagattc cagcaacagg
     2881 ccgccgatag accgggtgag ggcgaggcga tggtcgcgga tgcggtcgag gaggctctcc
     2941 acgttgtggg tgaaggcgac gatgtcgtcc agcccgaaca gaccggccga gcccttgatg
     3001 gtgtgggcgg cgcggaagat ggcgttgagg gtgtccggat cgtccgtgcc gtgctccatc
     3061 tccagcagcg cttcctccat ctgctggagg agttcgcggc tttccacgat gaaggtctgc
     3121 agggcgtcgt cgagttgaat gctcatgatg tggatccgtc cctcagcgtg cgccggccag
     3181 ggcgtccttg aaaaagtcca tcaggcggca gaactcgaag gcctcgagga tgcgcgggct
     3241 gaccgccgcg aagcgcagca cgcgctcggc gcccttcgcc tccagggcca gggaggccag
     3301 caactggatg ccggcggcgt cgatgtcgct gacgccggac aggtcgagca ccacgtcgcc
     3361 cggcgcctcc atggcaatga gcaggcggtc gcgccagtcg cgggcggtgt agatggtcat
     3421 ttcaccggcg atgaccacgt gtgccgtgcc cagaatcgtc tgttccgatg cgttcatgtc
     3481 ggtctcctcg ggagtcgggg cgttcagggc aggatcagct tggccacggc cgtgagcatc
     3541 tgctggggct ggaagggctt gaccacccag gccttggcgc cggcggcctg gccttcgcgc
     3601 ttcttgtcgt cgcctgcctc ggtggtgagc atgatcacgg gcgtgaagcg gtaggccggg
     3661 acctgcttga gggccttgag aaaactgatg ccgtccatgt tcggcatgtt cacgtcgctg
     3721 atgatcaggt ggatcttgcg gccgtcgagc ttggtgagcc cgtcgcgacc gtcgcagcct
     3781 tccacgacgt cgtatccggc gccccgcagg gcgatggaga caacttggcg caaggaggcc
     3841 gagtcatcca cgatcaggat ggttttagcc atgtcggttc ctcagaagaa agtgatgccg
     3901 gcgtcttccc gcgcggtcac cgggttggcg ccacgttgca gcgcgtgttg ttcgggggtg
     3961 gtgaattggc gcgccatgtc gcggcgccag gcgatggggt caagggggtc gggatgcagg
     4021 ccggcggcca gttcggcgcg gcagctgcgg acccgttgct gcaggcggtc catgtcacca
     4081 gagacgctgc ccagcacctg gctgacccgg tcctggaact gcagggcgac cagcacatcg
     4141 ccgatctcgg tggcgacggc gtggctctcg gcggacacct cgcgggcgcg ggccacgacg
     4201 cccccggcgg cgccgcgcag gcgctccacc acttcgccga tgcgccggcc ggcgtcgctc
     4261 accagctgcg cgtcgctctg ggcataggcg cgggacacgg acagggcatc gcccatggcg
     4321 ctggtgacgg tggcgatgat gtcgccgatc ttcttgccgg tttcgccgga ggcggtggag
     4381 agcttgcgga cctcgtccgc caccaccgcg aagccgcgcc ccgcttcgcc ggcgcgggcc
     4441 gcttcgatgg cggcgttgag ggccagcagg ttggtctgct tggccacgtc gcccacgtcg
     4501 cgagccatgc tctgcaggga cgaggtcagg cgggcgagtt cttccacctg accgaggagc
     4561 acgtccttgt tctcgagggc gcggcgcagc tcggcgacga tgccggagag ttcggcttcg
     4621 ctggcattga ggatggtcgc cagattgccg ctgccatcgt cgctggcgct gcccgcggcc
     4681 gattccacgc ggcgggacag atcaccgaag cgaaccgaca gggcggtgat ggcctcctcg
     4741 gtctggctgc ggaccatgct cacctggtcg cgccacaggg gcaggacgtc ctcgcacagg
     4801 cggtcgaggc cttcgaccgc ggcgggcgga gcgtctgcag ccggcccgta cgccggttcg
     4861 ggcgtcggca gcagcggccg ggccgtacgc caggccatcg ccagcacccc cagggctacg
     4921 gccagcgagg cgccgagcac ctccaggcgg ccgcccgcgg ccagtgtcgg cacgcccgcg
     4981 accagcaccg tcacgccgta ccacaggatc ggcatcgcgc cgaccgggcc ttgcgcgccc
     5041 gcttgcttca tcgtgtgagt ctccatgttc aaaccccctg ctccgttgca ccactgcggt
     5101 gacgccgcca ctggccgcga aaggccttca ccagcgccag cagctccgcc tcatccaccg
     5161 gcgtgcgcat caacaccacg tcgtccggca gccgtgcgcg cacacccatg cagtccgcct
     5221 ccgaggccac cagcacgatc accagcatcc cgcgggtccg cgcgccctcc accagcgtgt
     5281 tgaccatccg gaagaaatcg atcccggtca ggcccaggtc ggagatgagc atctccggcg
     5341 cttcgtcgcc gatgcgcagc agcgcatcga agccgttccc cgccgtcacc accacgcagt
     5401 cgggcaacat gccttccagc tgctgcccca gggcttcccg cccggcttcg tcttcgcgca
     5461 gcaccaggat gcggaagcgc ccacccggcc cgaacgcagc aagccgctgc tcttccacca
     5521 gtcgctccac cgactcccgc aggatccggc gatgcccacc cggcgtcttc caggcccgca
     5581 gacgcccctc ctccacccac aactgggcag tgcgtacgct gattcccagg cgccgcgcgg
     5641 cgtcacgggt agagcaggtc agggaggtcg ggtcgtctgt tttcatgatt gccgcgtcgt
     5701 tttgttaata atactatttt tatcgtttta tacaagtatt tttttatttt ttgtgtttta
     5761 gtcgatcttg aagcgcatcc agcggacatc tcgtgcccca gacaactgcc attactatcc
     5821 attttttcag tgttttagag cgcacaactg catctttgcg ggctcacgaa cggaatccga
     5881 aggccccgaa aacgcatttt tcgttttgct gtttttcatg tttttgtgtt tttagcaggc
     5941 aacacaccgg cgtgagcggc acggtggagg gccatcgaca caaggaggcg gatgcgcagg
     6001 aacgcggtca ggtcggcact ggtcttccag caggcgcgcc cgcaaggcac gtcggcccca
     6061 cgccggtgca ttcccttgcc aaatcaagga cgtggcgcac acaggcgtca ttcgctcttg
     6121 attttcgacc ggaggagaac tactacatgt agtagtaatt tttgaaatca gacactagct
     6181 ttatttttaa gctattgttt tttaatgatt ttattttttg ccgaaattcc agcgtcggag
     6241 taagctatgc gccgttcgac acatctcccg atcacctata aaaaggccac gagacaccct
     6301 atgagcgcta tcgccaaaga cgctgacgcc cgcactgccg ccaccctcgc accgcaggag
     6361 atctccggcg aggtccttct cgaaaagtac gccaagggca acgaacagac cgtggccgac
     6421 gtgcgcaccc gcgtggcgcg ggcgctggct gccatggaaa acgaagacaa gcgcgcccat
     6481 tgggaagcgc gcttcctcga agcccaggaa cggggcttcg tgcccgccgg gcgcatcaac
     6541 tccgccgccg gcaccaagct ggccgccacc ctgatcaact gcttcgtgca gccggtgggc
     6601 gactccatca ccgaatccgt ggacggccgc tgcggcatct acaccgctct ggcccagtcc
     6661 gccgagacca tgcggcgcgg cggtggcgtg ggctacgact tctcctccat ccgcccgcgc
     6721 ggcgcgatgg tgcgcggcac ccagtccaac gcctcgggcc cggtgtccta tatgcgtgtc
     6781 ttcgatcgct cctgcgagac cgtggaatcc gccggctccc gccgcggcgc ccagatgggc
     6841 gtgctgcgct gcgaccatcc ggacatcgag gacttcatcc acgccaagga ccacggtgac
     6901 ctgaccaact tcaacgtctc catcgccgtc accgatctct tcatgcaggc cgtggaagcc
     6961 ggcaccgacg tggaactggc ccacaaggcc gagcccgctt ccgacatcaa ggccgctggc
     7021 gcctaccagc gggaagacgg cctgtgggtg taccgcaagg tgcccgcccg cgacctgtgg
     7081 gaccaggtca tgcgctccac ctacgaccac gcggagcccg gcatcctgtt cctggatcgc
     7141 atgaaccggg acaacaacct ctattactgc gagaccatcg aggccaccaa cccctgcgcc
     7201 gagcagcccc tgccctccta cggctgctgc tgtctgggct ccatcaacct cacgctgtac
     7261 gtgaaggacc ccttcaccgc caaggccgcc ttcgacttcg ccgcgtttgg caagaccgtc
     7321 gagacctcca tccgcatgct cgacaacgtg ctcgaagcca cccactggcc gctcgagcag
     7381 cagcacgccg aagcccagtc caagcgccgc gtgggcctcg gcttcaccgg gctcggcaat
     7441 gcgctggcca tgctatgcct gcgttacgac accactgaag cccgcgccat ggccacccgc
     7501 atctccgaat tcatgcgcga ccaggcctat ctcgcctccg tcgaactggc cagggagcgc
     7561 ggcgccttcc ccctcttcaa tgccgacctc tacctgtccg gaggcaactt cgcgtcgcgc
     7621 ctgccccagg acatcaagga caagatccgc aagcacggcc tgcgcaactc ccacctgctg
     7681 tccatcgcgc ccaccggcac catcagcctg gccttcgccg acaatgcctc caacggcatc
     7741 gagccgccgt tctcgtacac ctacacccgc aagaagcgca tggccgacgg caccttcaag
     7801 gaatatgccg tcgaggacta cgcctggcgc ctgtacaagc accagggcgg tgacgtcaac
     7861 aagctgcccg agtacttcgt caccgcgcta gagatctccg ccaaggccca caaggacatg
     7921 gtggcagccg tcgctccgtt cgttgatacc tccatctcca agacggtgaa cgtccccgag
     7981 gactacccct acgcggactt cgaagatctc tacctggaag catggaaggc cggcctgaag
     8041 ggtctcgcca cctatcggcc caacagcgtg cttggttcgg tgctgtcggt gagcaccccg
     8101 tccaccgagc agaagcagcc ccaggacgtg gagatctctg acaccaacaa gcgcctctcc
     8161 atcaagagcc tgcctgcgcc ggtgctgtcc tccctgcgct ggcccggtcg cccggatctg
     8221 ccggacggca acgccgcatg gacctacatg ctgaacacgc cccagggcaa cttcgccctc
     8281 ttcgtgggcc acatggaccc catcgaggac agccccgcca agggcttctt cccgttcgaa
     8341 gtgtgggtga acggtgccga tcagccccgc ggcctcggcg cggtggccaa gaccctgtcc
     8401 atggacatgc gcgccaatga ccggggctgg ctcaagctca agctcgagac cctcgccaag
     8461 accgtcggcg aaaagtcctt cgagatgcgc ttcccgcccc atggcgaaca gaagctgatg
     8521 cccggcgtcg tctccgcctt cgcccaggtg gtccgctacc gctgcgaaaa actcggcgcc
     8581 ctcgaaggcg atctgccgaa cccggtgctc gacaccctgt tcagcctgca ggagcccaag
     8641 accggcaccg acggcaccct gtcgtggaca gtggacatcc agaacccggc caccggcgaa
     8701 gacttcgtcc ttggcctcaa ggaaatcacc ctgccggacg gcgtcacccg cccctactcc
     8761 atgtggctgt cgggcaacta cccgcgcgcc ctggacggcc tgtcgcgcat cctgtccctc
     8821 gacatgcgcg tcatggaccc ggcctggatc ggcatgaagc tgcgcaagct catcgattac
     8881 ccggaacctc tgggcgactt catggccttc gtgcccggca cccgccgcca gcagaactgg
     8941 ccgtccaccg tcgcctacat ggccgcgctg atcatccacc gctacgccat gctcggcgtc
     9001 ctcgacgaag ccggctaccc gacccgcgag atgggcatcc tcgaagctcc ccgcgacgac
     9061 aacgagccca agctgatgca gggtgccctg tgcaacgagt gcggcaacta ttcggtcatc
     9121 cgcaaggacg gctgcgactt ctgcaccgcc tgcggcgccg tggggacctg cggctaaggt
     9181 cccgccgccc cgctgggggg ccttcgcctc agcgaacggc tccggtgtgg gcggggcagg
     9241 cggtcatgct gaacaggaca acgcaccacg ccaccggcaa gccgtcaaaa cgcaacgccc
     9301 accttgatcg aggtgggcgt ttttcatgcg gatcgccgaa cggtccgtcc ggacgtgtgg
     9361 ccgtacaggc tattccgcac agcgcgtcat ctgtgcctcg ccacgcgtcc agcaggggcc
     9421 cgattttgaa gcatccggcg cgggcgcacc atgcccatcc agcctgacga cccgggttcg
     9481 gatcgccgaa acacccgcgc tgtccaccgt ggcgaacact gcccaacccg taatgttgtc
     9541 ctcatccgaa aagccgtcga acacgaaatt tcccagcgag tacagaatcg gcttgccacg
     9601 gtagatttcg tagtcctgaa cgacatgggg atgaccaccg atgaccgcgt cggcacctgc
     9661 gtcgatgatc gcccgggccc gcgatctctg atgttcattc gcctgcggct gatgttcctc
     9721 tccccaatgg gggatgacga tcaccacctc ggcgccacgc gctcgggcat ccgcaatatc
     9781 cacggcgacc tgacggggat cgagccacgc gacacccggc agtcctggag ccgcggcgaa
     9841 ccagcgcgga aagaagtcca gatagccgag cagcccgatc cggacgccct ggcgttccac
     9901 gatgagcgcc cgatgggctt cgaccagatc gcgtccggcg ccgacgccca gcgatcctga
     9961 ctgacgcagg gacgcgaggg tctccaccag ggcctgtcgt ccgaaatcca ccgcgtggtt
    10021 gttggccagt ccggccacct caaacacaga tgagaaggcc actacgccgg ccgccgtggt
    10081 ccggaaactg aagggcttgg cgggatcggc cttaccgctg cggccacccg ccgactcgaa
    10141 attggcgacc cgaagatcgg cgggcgccag gatgctgcgc acccgcctga aggggtcgcc
    10201 cccccgcgca atgagccggc cggtcgcctc cgtcctggcc agcatgacgt ccccgacaaa
    10261 ggccaccacg gcgctgctct cggccgccac cggcgccgcg atcaggcagg ccacgaagcc
    10321 gagccccagc ctgttcatgg gtgatcgtcc ttgcaggtat aggtgaggct ctgcccaagc
    10381 cggggcgcca caacgtccac gcgcccggta tcgaatgggg attcgaccgt gtgggaggac
    10441 agaatcctga aatcctggcg gccggcctcg gcgtagacct cgatcaacaa ccaggcgcga
    10501 ccttggcgtg gggtcgtcaa ggcggtacgc acccggaaca cacccaacgt cgcccattgg
    10561 cccgcgagcg acgtttcacc ggccggcacg tccatccgcc ggatcatgct gccctgctcc
    10621 agcgtgcatt caacggccag ggcagcggcc gagctgagca gcccgagcgc catcagaagg
    10681 gcggtcctga aacacacttc gggctttctg agaacggaca tggaaaggcc tgcttttaac
    10741 aggccgcatt ggtgggttcg gctcgtcacg gtgcgcgaaa ccggcagcca agtcaattca
    10801 gggcgatgcc gggagcagga tgctcgggcg gctcccggca tcgtatcggc agtctgggag
    10861 gccctgccag cgcgcgccgt cgccacgcac cgcgacgccc cttcactgaa ccctcggcct
    10921 gccgttgact cgaaggcatt gcggcccttc gcggctgccc accaggaagg gaggatcacg
    10981 atggaccgga tcgacgccag gccggaggcc ttcgctcacc tcgtgtatcg ctacctgtgg
    11041 ccctacgcgt atttccgtga cgtgacccgc ggcagctttc tggagcggca gcagaactac
    11101 cgccacaacc ggcgcatggg catgcacctg cccggcttca tgctcaagtg ggcgtgcctg
    11161 acggtgttct tctatctgct cggcagcctc tgcgaagaac tgctggaatt ggtgttgccg
    11221 gcagcctgct gctatgtgac gggcgcgtgg acactgacca tcctggtcca gctctgcgtg
    11281 gcatggttgt ggctgaagcg ttttcccgac attccgaggg gatgaggcag cgacggcgca
    11341 ggtgccggat cgccgccatc cgtgtcaggg gcggttcgga tcgaaaccgg tttcccgata
    11401 gatcgcgcgg atcagactgg caccaatgcg ggcctccatc cgggcatgca ccggtatcag
    11461 aacccgccgg aaggcctccc gttcctcctt gctgggcacg tggatctgcg tcttgccgct
    11521 cttgcggatg agctcaagag agcgctcgtt ctcttcctgg gcgatccggt tggcgtagac
    11581 cgtcgactcc ttcatcgcct gttccagttg gctgcgcaca tcgcccggca ggccgtccca
    11641 gaacttcttg ttggcgatga cggcgtaacc caggtagccg tgatcggtca ggcacaggta
    11701 tttctgcacc tcatgcatcc tctgcgtata catattggag tggggatttt cggtcccatc
    11761 cacgaccccc gtccgcaggg cctgatacac ctccgagaag gccatcacct ggggcagcgc
    11821 gcccagcgcc cgcacctgct cctcgaggac cttggagggc tgaatgcgca gcttcttgcc
    11881 tttcagatcg gccggcgtac ggatgggcgt gttggctgaa aatgacttga agccgttatc
    11941 ccagaaggcc aggccaatga tgcccttccc gtccagctga cgcaggatgg acgcgcccac
    12001 cggtccctgg gtgacccggt gcagatccgc atagttgtca aaaatgtagg gcaggtcgaa
    12061 gacttcgaat tccttccccc ccagtgggcc gaacttcgcc agggacggcg ccagcatctg
    12121 tacggcgccc agttgcagcg cttccagctc ctccttgtcc ttatagaggg agctgttggg
    12181 atatacctcc accttgaccc gcccgcgggt cagctcctcc gcgcggcgct tgaagaattc
    12241 cgaagccttg cccttgggcg tgtcggccgc caccacgtga ctgaacttga tgacgatggg
    12301 cccctctgcc cccgcaggca gggccaggag ctgcagaatc agggccagca atgcagtacg
    12361 cagcatcaac atgcggtcat tccgtccata atcgcccctg aaaagcatcc acttggaaac
    12421 gtctgccaga ttacaacgga agacccggat ttcgaacaga tccgccagta ctttctgcaa
    12481 gcttactttc cacccacccg cccgttgtgg aaactgcgaa tggcatgagc tgcccgaacg
    12541 gtcgataaag gagactcccg actcttgtcc cagaatccct tgaacttccg tccaccgtcc
    12601 cctccgccgc cgagctggta ttggggcctg ccctatgtgg ccgtggtgct cttcatcgcc
    12661 accgtggccc tgctgctgtg gctgacccat cgtcaggacg cggaggagca gcgcggcatc
    12721 ctcatcaacg acgtgctgtg gatggagcag aacctgtcct tccagttcga gcgcaacgcc
    12781 gccctgctca cccagctggg tccggagctc ctcggcaaaa ttgccgacga ctccaccgaa
    12841 gcccgcctca agcaagccct cgatcccgac accggcctgg tacgcatcct gtggttacgc
    12901 gaggatggtc gcgtaaaagg tgccgagccc ccattcaccg atacccatct ggtgggcgaa
    12961 gccagcggta ctttcccttc cgacgcgacc tcgcgcctgg cccgctccat gggccgtgcc
    13021 acctacagcg acacctatgc ggtcatcgac aaccaggcgc agttcgaggt gcacgtcccg
    13081 gtgttcgagc gcgggcgcta cgtgggcgcg gtggtgggcg tgtattccct caacgaactg
    13141 ctccagcacc agatcccgtg gtggttctcg gagcgttacc gggtgacggt gaccaacaac
    13201 ctgggcgacg aaattgccgc aaagtccaag gtggcgccgc tgtccaccct ggcctacgag
    13261 attcccttcg agccacccgg ccacggcctg tcggtgagcg tcaccgccta caagagcgaa
    13321 gtacgctggg tgcccctgct gctgatgggc tccctcgtca tcctgggctg tgccatcgtg
    13381 tggagcctat gggcgctgcg gcgtcacatg cgccgacgac aggccgcgga acaagccttg
    13441 cgcgccgagc acgcctttcg caagtccatg gaggattccc tcaacaccgg catgcgcgcc
    13501 cgcgacctgg aaggccgcat cacatacgtc aatccggcgt tctgccgcat ggtgggcttc
    13561 agcgcggacg agctcatcgg catgcgtccg cccatgccct actggccacc ggaagacctg
    13621 gaacgcatcc tggatgtaca cgccatgatc ctgtccgggc acgcaccgtc ccagggggtg
    13681 gaattccgcc tcatgcgccg ggacggtacc cgcttcgatg ttctgctgat cgaggccccc
    13741 ctcatcgacc ccaacggccg ccacatcggc tggatgggtt cgatggtgga cgtcaccgaa
    13801 cagaagcgcg cccaggaact ggcccgccag cagcaggaac gcctgcaggc cacggcccgt
    13861 ctcgtcacca tgggcgagat ggcctccacc ctggcccacg aactcaacca gcccctcgcc
    13921 gccatctcca gctacaacac gggctgcctg aacctcatcc gccagggcga cctgccccgc
    13981 gaggaactca ccggcatcct cgaaaaaatc ggccgccagg cccagcgcgc ggcccagatc
    14041 atccgccgcg tgcacgattt cgtacgccgc cgcgaaccca acttcgttgc ggccgacttc
    14101 aacgccatcc tccgggaagc ggtcgaactg gtcgaaccca cggccgaacg ccgcggcgtg
    14161 agcatcgact gcgacttcac gatgcccctg ccggcggtga gtgcggatcc agtgatgatc
    14221 gaacaggtgg cggtcaacct catccgcaac ggtatggacg ccatggccga cacgcccatc
    14281 gacgagcgcc ggctgcaatt gcgcaccgcc ctgcgggacg gcatggtctg ccttacggtc
    14341 gaagaccggg gcgccggcat tcccgcggac gtggccgaaa aactcttcgc ccccttcttc
    14401 accaccaaaa gcgaaggcat gggcatgggc ctcaacatct gccgctccat cgccgagttg
    14461 catcggggtc gcctggcata cgaagcccag ccagccggag gcaccgtttt ccacttttcc
    14521 gttccggtcc tgcagccatg aacacacccc ttgcccacat cgtcgacgac gatgaagcca
    14581 tccgcgacgc cctcgcctgg ctgttccgta cccgcgccgt cgaatgtcgc gcctgggaat
    14641 ccggcgaagc ctttctcgac gcctggcagc cggactggcg cggctgcatc gtgctggaca
    14701 tccgcatgca cggcatgaac gggctggaat gcttcgacgc cctcaatgcc cgcggcaacg
    14761 ccctgccggt gatcttcctc actggtcacg gcgacgtacc catggcggtc ggcgccctca
    14821 aggccggcgc cttcgatttt cttgaaaaac ccttcaagga caacgacctg gtggacgtgg
    14881 tcctccgcgc catcgacagc gacacacgcc gccacgccag tcaggccacc caggccgaca
    14941 tcgcgggacg catggcccag ctcacgccgc gggaacacga agtcatgcaa cgcgtgctgg
    15001 ccggcaagtt caacaaggtg attgccgacg aactgaacat ctccatgcgc acagtggagg
    15061 ttcaccgttc ccgcgttttc gagaagatgg gcgtgcgctc agcggtggaa ctggcccagt
    15121 tcctcgcgcc cggccgagaa ggacgggact agaccagtcc gttccactcg ggcacgacgc
    15181 cgggcgcatc ggccgggcag cggaattcgc ccgccacacc aacgcccgcc acccaggcga
    15241 cccggtcgcc gacccacaac acgggcagac gcggccgcag ccacggcgga atgccggcct
    15301 cctgaaagag attcttgagg gtgcgcgacg gacctgccgg gtgcagggac agacgttcgc
    15361 ccccacgccg tcggtcaatc cgcaccggcg caccgatcaa cgcggcacgc atgatccccg
    15421 cccccgtcac agcccgaaaa tccaaggccg ccccggccca cctcagcgcg gccaaagacg
    15481 tcagtgcaag accttcgggc gtgccctcgt cacgagaatc gccctccagc caccagcggt
    15541 cacggtatac acacagcgcc tgtgtcccga gccggcaacg cagaccttga ggggcgccga
    15601 cgcttcggaa ctgacgctcc gcctcccgta gccggtcttc gtcgggcaga atcgggtcag
    15661 cgtccacaag cagagatcgc agcagattgc gccagcgcgc cgacgacagg tgcaaagacg
    15721 cggcataggc aagacgcgtc gatccatcgc tacagctcag ccgcgcacga tcctcgttcg
    15781 ccatctcgtc cagcagatcc gatgcttcac ccgccagacg cccaagccga gccaacgtcc
    15841 caacactccc cgggaaacgt actgacagcc gcggcatcag ttcatggcgc aggtagttgc
    15901 gggtgtgggc atggtccata ttgctcggat cctctatcca taccagaccc acagcccggg
    15961 cataggcttc aagttcggca cggtacgtgt tcagcagagg gcgccacacc agcccgtcgg
    16021 actgggactg gatctcccgc atccccgcga gacctctgac gccggcgccg cgcacggcgc
    16081 ggaacagcac cgtttcggcc tgatcgtccc gatgatgggc aagggcgacg accccgccct
    16141 ccgccagcag cgccgcccgg cgcgccatcc gcgccgccgc ctcgatcccg gcgggactct
    16201 cgcggtcgac cacgacgtcg accaccctga gcggaacatc cagattcgcg cagaacacgc
    16261 cacagtgcct accccaccga tcggcgccgg gtaccagtcc gtggtggacg tgcacggccg
    16321 acaggccgaa tccttcggcc ctacgcacgc tcgcaagcac atgcagcaat acggaagaat
    16381 cgagcccccc gctcagacca acacgcagcc tcttgcgcgc cggagcgggg agccgccgcc
    16441 acgaacgggc gacctgatcg accagttcag tcgacggcaa tttccttgaa ctggccgtag
    16501 ctcatcagac gctcgaagcg tcgctccagc aactcggtcg gcgacaattc ggacacctgg
    16561 cgcaaagcat cctgcagagc ccgcttcaga ctctgactca tcacccgatg atcacgatgg
    16621 gcgccgccta ccggttcgga caccacctta tccaccagac ccagggattt cagccgcgac
    16681 gccgtgattc ccatggtttc cgctgcgtcc gccgcacgtt cggcgctctt ccagaggatg
    16741 gacgcacacc cttccggaga gatcacggaa taggtggagt attgcagcat cagcacctga
    16801 tcacccacag caatggccag cgcgccgccc gagcccccct cgccgatcac ggtgcagatg
    16861 atgggcacct tcagctcggc catcacgaac agattatgtc caatggcctc tgactgcccg
    16921 cgctcttccg cgtcaatgcc gggataggcg cccggtgtat ccacaaaggt gaagaccggc
    16981 agtccgaact tctccgcaag acgcatcaga cgcagcgcct tgcgatagcc ttccgggcgc
    17041 ggcatcccga agttgcggaa gatcttctcc ttggtgtcgc gccccttctg atggccaatc
    17101 acgacacagg actgcccgtt gaagcgggcc aaacccccaa cgatcgcctt gtcatctgca
    17161 aaggcgcgat caccgtggag ttcctcgaag tctgtgaaga tgttctcaac ataatcgagg
    17221 gtgtaggggc gctgcggatg ccgcgccacc aaagcacact gccagggggt cagctttgcg
    17281 tagatatcct tggtaaggcc atgattcttt tgctcgagtc gggcgatttc ttcggagata
    17341 tccacagcgg aatcatcctg cacgaaacgc agctcttcga tcttggcttc cagttcggca
    17401 atgggctgct cgaactcgag aaaagtggtc ttcatctgca gagctcccga cgataatttg
    17461 agattctacc ctaaggaagg tcgagctctt ccttgcgatg cagctcagcg catggccgct
    17521 gtacattttc cttcttattt tccataggca aacccccgac caatgatttg gccgggggtc
    17581 tgatggggtt agtctga
//
LOCUS       DAOQMM010000078        17548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1220_length_17548_cov_44.163494, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000078 DAOQMM010000000
VERSION     DAOQMM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_17548_cov_44.163494"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLH85_12345"
     CDS             complement(<1..192)
                     /locus_tag="PLH85_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65402.1"
                     /translation="MSIPAKGLSTGAQILGALAQTLSTRAQTLRTDAQIVSAPAQMLR
                     TPAKTLRTPARTVRTSAQTL"
     gene            complement(517..2646)
                     /locus_tag="PLH85_12350"
     CDS             complement(517..2646)
                     /locus_tag="PLH85_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65403.1"
                     /translation="MEPNTENLLGIIADSPDFIGVIDLDGHLKYHNKAACRLLGRPDD
                     ADLTALSLPTVHPPWAAQLLRDTAIPTALATGHWEGESAILRPTGREVPVWQEILPQR
                     DVSGAPTLLSIIIRDISAQKLSQNLEKHRSTVLQFLVDAAPLATVLEAIVVGIERIDP
                     TALTSILLLDDTGRHLLKGAAPRLPDFYNKAIETLEIGFGRGSCGTAAATGSRVIVED
                     IATHPYWAPYKELAAHAGLASCWSEPIRDSHQKVLGTFAIYHRHPSAPTEEDFALIGQ
                     ASSLASIAIERRRTEMELERYRNHLEERVEERSRTINDLNRELEQRVRDAENATREKS
                     EFLANMSHEIRSPLNAIIGLTHLMRRSATDADQIDKLGKISTAGHHLLSVINDILDFS
                     KIESGNLELESNIVELRTLAGNITSMLEIAARDKGIALKVDTGDIPRQVRGDSTRLTQ
                     AFLNLANNAVKFTEHGTITLRIRLLDETADTVLLRFEVTDTGIGVAPEILPTVFSSFR
                     QADSSTTRRFGGTGLGLTITRRLAELMGGEAGAESTVDVGSTFWFSARLAKVLESDAA
                     RRAAVPPSEIEENLRQHFAGTRILLVEDDIINQMVGQELLESVGLTVDIAHDGVDAVE
                     RMRGPDAERYALILMDMQMPRLDGPGATRQIRALPAQPKRPILAMTANAFGEDRERCL
                     SAGMDDFITKPVNPDILYAKLLQWLST"
     gene            complement(3187..4101)
                     /locus_tag="PLH85_12355"
     CDS             complement(3187..4101)
                     /locus_tag="PLH85_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNB65404.1"
                     /translation="MIVRPHQHWFRRLFDWHGSVLPNILFRLSLNVMMGIVAVIALPW
                     YEQLDIHLTTAPFSLLGVAIAIFLGFRNNTSYGRYCEARLFWGSLLVTTRSLLRQWKS
                     ALPDAASAREFAGLLLAFTWSLKHQLRGSSPQDDLRRLLPERYLADVSASPLTAHRIL
                     LHLDLWLGDRRRAGQISDVLYQSFDANLNELSRILGGLERLASTPIPFAYSLILHRTV
                     YLFCTLLPFALVSDLHAMTPVVSVFISYTFLSLESIAEELEDPFGTAPNDLPLNALCT
                     TIEIQLKELNDDHPLPPRPQPDAHFNLT"
     gene            complement(4360..5358)
                     /locus_tag="PLH85_12360"
     CDS             complement(4360..5358)
                     /locus_tag="PLH85_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNB65405.1"
                     /translation="MTPPALPRLLRTLHKEWFLVGMIAAVLLASLAPNLGRSGGVLHG
                     DRVADAGIALVFFLHGLGISLTAFRNGLGRWKVHLVVQLCTFVVFPLVGLAAYALLGR
                     WLPAGLALGFCYLCALPSTISSSVAMTGLARGNVPAAIFNATLSSLLGIVATPLLVAA
                     FTGVQGDGLPFMASVIGIARLLLLPLVAGQLMRPLLHAWHTRHKRLTTLLDRWVILLL
                     VYSAFCDSVASGLWQHNGLASLGIAFIGAGAFLAFILAFSTWLVRRCAFPVEDEIATV
                     FCGSKKTLASGVPMAKLLFGAHPSIGLILLPIMLYHQLQLFVCSLLANRYAARSAG"
     gene            5490..6254
                     /locus_tag="PLH85_12365"
     CDS             5490..6254
                     /locus_tag="PLH85_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB65406.1"
                     /translation="MKRILPALAALPRPVFGRNESLAPGMWTDVHTHPWCQLSYAVSG
                     VLAVATPQGDYVAPPHYAVWIPPEVPHQVFNRGRTEMRSLYLENGAPGLPTACAVLEV
                     APLLRELILKVGALPPDYVPDGPAGRLVAVLRDELAAARQAGFALPLAKDRRLLPIQA
                     ALQDNPADARTLAEWATQVGASERTLARCFVKETGLGFGDWRRRLRLLLSLSALEAGD
                     SVTTVALAAGYDSTSAFIAAFREVFGFTPGSLPGRS"
     gene            complement(6264..8729)
                     /locus_tag="PLH85_12370"
     CDS             complement(6264..8729)
                     /locus_tag="PLH85_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65407.1"
                     /translation="MSTSVITMSPPHDAPATIEEALLSRDALYRNIIASAADGFFTVD
                     ARYRFLDVNQAFCTLFGCPRETFIGRSPLDFVTPASRPELTRQIGRVATTQRRVYELE
                     GLRPDGSTFPALISTVTHRNVRDEIVGTLGFITDLGPAYAARARVAQSEAELRAVLDN
                     LQDTYYRTDADGCLVRVSPSVRKLLGYDAEELIGTPIARLYTRPTERDVFLQTLEEAG
                     GAVEHYEASLRHKDGHEVWVATSAHLYRNPDDDTVGVEGTTRDISRERAQRVQLQLAA
                     TVLAATREAIMITDHDNRIISVNPAFTQLTGYQPEEVIGQGPQLLSAQRHSIAFYSQL
                     WDALAGDDHWSGEVWNRRKNGNYFPQWLSLSAVRDPAGKLLHYVAIFSDLSDRKAAEA
                     RIEFLAHHDALTCLPNRLLLKERAQQCFVQAERNRAKPALLFLDLDRFKDIIDSLGHP
                     IGDRLLQAVAERLRSATRESDLVGRLGGDEFLVLLNGIRDTEDIAPVVEKIMQALAEP
                     YHADGHELLCTCSVGLAVFPDDGTDFDTLAKHADAAMYEAKAAGRATFRFYSQRMNED
                     ALARLDLQKGLRRGLERNEFILHYQPLVDLADNRIIGAEALLRWQPPDRPLVPPGHFI
                     PVAEDSGLIVPIGAWVLREAFTQARAWQDAGHQLVMAINLSPLQITRGDLVDTVRALL
                     ADTGADPHRIELEITESVLLHDTEQVLAVVRQLRQMGLRLSIDDFGSGYSSLAYLRRF
                     AVEKLKIDQCFIRDLPTDPEDGAIVRAVIQMAKSLKMTVLAEGVESADIAHDLRLLGC
                     DLAQGYHFGRPMPAQAFLDLLGA"
     gene            complement(8781..9614)
                     /gene="metF"
                     /locus_tag="PLH85_12375"
     CDS             complement(8781..9614)
                     /gene="metF"
                     /locus_tag="PLH85_12375"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNB65408.1"
                     /translation="MTPNLSIEFFPPQTPEGADKLRVVRQKLAVLKPEFFSVTFGAGG
                     TTQDRTFDTVFEIRSEGHEAAPHLSCIGSTREHIREILHRYRDAGIRRIVALRGDLPS
                     GVGFAGELRYANELVEFIRAETGDHFHIEVAAYPEYHPQARSPRDDLLAFQRKVAAGA
                     DSAITQYFYNPDAYLHFRDECDKLGITVPIVPGIMPIVSFSKLARFSDACGAEIPRWM
                     RRKFESFGDDSDSIRAFGLDVVTGLCEKLLAAGAPGLHFYCMNQSGPTLALCERLGIG
                     A"
     gene            complement(9687..11099)
                     /gene="ahcY"
                     /locus_tag="PLH85_12380"
     CDS             complement(9687..11099)
                     /gene="ahcY"
                     /locus_tag="PLH85_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNB65409.1"
                     /translation="MNAVAETFTDCVVADLSLADWGRKEITIAETEMPGLMAIRDEFA
                     KTRPLQGARITGSLHMTIQTAVLIETLIALGAEVRWASCNIFSTQDHAAAAIAAARIP
                     VFAVKGESLTDYWDYTHRIFEWADGAYSNMILDDGGDATLLLHLGARAEQDLSVLDSP
                     DSEEATVLFAAIKAKLKTDPTWYSTRLARIQGVTEETTTGVHRLYQMHERGELKFPAI
                     NVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVCGYGDVGKGSAQALRALS
                     AQVWITEIDPICALQAAMEGYRVVTMDYAADKADIFVTATGNYHVIDHHHLVAMKHNA
                     IVCNIGHFDSEINVASLEAYPWEEIKPQVDHITLPSGHRIILLAKGRLVNLGCATGHP
                     SYVMSSSFANQTIAQIELFTKTADYPVGVYTLPKHLDEKVARLQLKTLNVALTTLTDA
                     QAQYIGVPVDGPYKSAHYRY"
     regulatory      complement(11192..11301)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(11351..11983)
                     /locus_tag="PLH85_12385"
     CDS             complement(11351..11983)
                     /locus_tag="PLH85_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNB65410.1"
                     /translation="MPTAPFAAPDTPPARSDLLRDLSMGLLLDELAFGIAVLARDRTL
                     IYASRTALAHFHPGGVLQILDDLVEAPNARDALLLQDALDDVQSGLRTYLSLGHGEHS
                     VDVAILPLTGMVTDSGTPAAIVFEQSTQTAGLSLYFYARAHGLTRAEERILGALYNGA
                     SVDDVATRIGCTPNTARTHVRRVLEKTGQPTLRALTARLGRLPPMAGQAD"
     gene            complement(12137..12979)
                     /locus_tag="PLH85_12390"
     CDS             complement(12137..12979)
                     /locus_tag="PLH85_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65411.1"
                     /translation="MAASPEPPQPEALAVRVWIARRRQNRDALDNITGPALSREAFLT
                     ALRQTFVPATPQLMAPLGLLAYFPTLPPVVDAYPDLPEETALVFYSSRDAYDIARRTP
                     SGRLYAASHGLYFDALAARWPERWQPGDASVPGQAYCGPAHRDGDWNEGQVWTFMLAW
                     QDAPVTDSWMRDLCAPCLAGIAPIRQWVAHYTDGCLVVWFHSPQPATAQALQIRQALR
                     DARLPGAPDTIYLHAARDVTVAALTEPGSGLDFHEDETLHVCYRPPIAPPPASPGPDA
                     SAPP"
     gene            complement(12988..13950)
                     /locus_tag="PLH85_12395"
     CDS             complement(12988..13950)
                     /locus_tag="PLH85_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB65412.1"
                     /translation="MSWTASDFERLDPKLFRGFLAVVRSGSVSEAARIASLTQGAVSQ
                     QIARLEERLKCQLFVRANGRILPTDAAQLLAEFAESYLQSSSSFLERLNAEFESIQGL
                     VAYSMPESCIHSPHFPQLLARRLEFPGIHLRVTLKPTDAVSRDVLGGEVDFGFVLEKR
                     HEKQLIHHPFCVEEYGLVAAPSLHPRPLSHLDDIVRLPYVCFPESAFYLHRWLSGLAR
                     REMSPDADDLSVRGEFSDLRGAIAMVKGGMGATVLPLHVVADDLAAGRLVRIRHRDND
                     PPVTQQISICRLAERRLPARVRRVIRWFLEMHADLQPVPADFLN"
     gene            complement(14013..15587)
                     /locus_tag="PLH85_12400"
     CDS             complement(14013..15587)
                     /locus_tag="PLH85_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65413.1"
                     /translation="MKPPIEVALMDEVRSCRDCTSLWRAPDRFGPFPLCDGAALDALP
                     AARADAPAPTPQAVTVEGFGLPDPAALRGCRKAPVMIVGINPNLTGFWTLPDAPRYAL
                     KGPSTVYARHRSLAEYARAHRYVTPDHHEYRMRPEDVETLIDATRHDLRAAGAGSVIP
                     VDKKHPAASPTGRQSHARHVTLGLQFDDGGTDTTQFDWLPDEHYVTLRARFAAGERIG
                     GVMTAASVQGRTIAVRADVGSSYYARAQEICRRSDLVPGEDISLHDMVACATQGWSDK
                     TGVDRDELSAACIDSRRHALRQIRQSAPRLLIFSGLEALGLFLRAVGLPAFPALGPDV
                     TVTRLLAGLKTELDTGALTRRLALHLPADGDLPPWRAHVVAVPHLSYPIRPIAFDAGV
                     ASPLWSRFAEHCPASFALIAQAPENSGTGAHALLDTATPSFRLLSDTWQTIDQLDEGD
                     ALYRLVEIGGDTRIEALASLIAQLRHSGDLPERAPDRAANARLLRSAGPCHFCPAFAV
                     PEGCIFEFADFANTEL"
     gene            complement(15584..16927)
                     /locus_tag="PLH85_12405"
     CDS             complement(15584..16927)
                     /locus_tag="PLH85_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65414.1"
                     /translation="MPTPSLPCLAIGGDTRHGAGRPGTGTDPWWSALALGASGLATIL
                     HSVDGRLECAGPTGLDGVPSLSLDDFLWTFGQACPLAFLVPHETPLTHDQVQACARRL
                     LGAALRHPPRLIVPATMLADVIATGFDTACSLNPYAEPSADDHQAWSSDSAAHRFPDL
                     PFPGGRSDGAYLLVRRGRSGQFQPDVWLTTDADLDARAQQSAILLDDDFTDGAASRRW
                     IFGYSNPVPESFILFGPPEAPAFIVDMPAGTGYSGGAAVLARALVTDFDARITFVSED
                     PAQGTTLELAALTIDPPRRSLVDRSHTADDKYRSLCFDVHGAPPYVSSECDENDGFRI
                     GWNLAGALARIDDSVTASSNHYNRYGEDVGGPLKGPISGELRLVRCGEFWASYFRLGG
                     ESAWRCSGALHIPDLPRRIHLRAGAKHWVKDGRPAPRNRVSFTTATVLIPAWSQP"
     gene            complement(17158..>17548)
                     /locus_tag="PLH85_12410"
     CDS             complement(17158..>17548)
                     /locus_tag="PLH85_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB65415.1"
                     /translation="ILRAQAAAPAEGPHFRRIASGAELDEILKTADRPVMLDFYADWC
                     VSCKEMERFTFADPAVARRLAGVLLLQADVTANSEDDKALLKRFDLFGPPGILFFDAH
                     GAERRAQRVIGFQAADAFLRTLDAALP"
BASE COUNT         2660 a         4846 c         7243 g         2799 t
ORIGIN      
        1 cagtgtctga gcagacgtgc gcacggtccg agcaggcgtg cgcaaggtct tggcgggcgt
       61 gcgaagcatc tgagcgggcg cgctcacgat ctgagcatcg gtgcgcaagg tctgagcgcg
      121 cgtgctcagc gtctgagcaa gggcgccaag tatctgagcg ccggtgctca gacccttggc
      181 ggggatgctc aaggtctgag tggggatgct cggggtctgc gcgcgggtcg gcacgggctg
      241 cgggccggtg cgcgcaggcg gggggcgcgc ccgacgtgcg ggggcggggg cgatgccgcg
      301 ggtgggggcg ggcactctac cctgcgcccg gtggcgatgg aggcggccat ccccgcggtg
      361 ggcccgcgcg gggctgccgg tgcgctgggg ccccaggcgg tgcagggaag gcggtgtgcg
      421 gtgcgaagtg cgccgccggg agggcaccag ggcgcagccg cccgcgtcgg gatggggcgc
      481 gtcaggcggg tgtcaccttc ccgccggccg tgtctttcag gtggacagcc actgcagcag
      541 cttggcgtag aggatgtccg gattgacggg cttggtgatg aagtcgtcca tgccggcgct
      601 gaggcagcgt tcccgatctt cgccgaaggc gttggcggtc atggcgagga tggggcgctt
      661 tggctgggcg ggcagggcgc gaatctgccg ggtggcgccc ggtccgtcga ggcgcggcat
      721 ctgcatgtcc atcaggatca gtgcgtagcg ttcggcatcg gggccgcgca tgcgctcgac
      781 ggcgtccacg ccgtcgtggg cgatgtccac ggtcaggccg acgctttcga ggagttcctg
      841 gccgaccatc tgattgatga tgtcgtcttc gacgaggagg atgcgggtgc cggcaaagtg
      901 ctgacgcagg ttctcttcga tctcgctggg cggcaccgcg gcgcggcggg cggcgtcgga
      961 ttccagcacc ttggcgaggc gtgccgaaaa ccagaaggtg ctgcccacat cgacggtgct
     1021 ctcggcgccg gcctcgccgc ccatcagctc ggccaggcgg cgggtgatgg tcaggcccag
     1081 gccggtgccg ccaaagcgcc gcgttgtcga ggaatcggcc tggcggaagg agctgaacac
     1141 cgtgggcagg atctccggcg cgacgccgat gccggtatcg gtgacttcaa agcgcaggag
     1201 gacggtgtcg gccgtctcgt cgaggaggcg gatgcgcagg gtgatggtgc cgtgctcggt
     1261 gaatttgacg gcgttgttgg cgagattgag aaaggcctgg gtcaggcggg tgctgtcgcc
     1321 ccgcacctgg cgggggatgt cgccggtgtc caccttgagc gcgatgccct tgtcgcgggc
     1381 ggcaatctcc agcatggagg tgatgttgcc ggccagggtg cgcagttcga cgatgttgga
     1441 ttccagctcc agattgcccg attcgatctt ggagaaatcc aggatgtcgt tgatcaccga
     1501 aagcaggtga tgaccggcgg tggagatctt gcccagcttg tcgatctggt cggcgtccgt
     1561 ggcggagcgg cgcatcaggt gggtcagccc gatgatggcg ttgaggggcg agcggatttc
     1621 gtggctcatg ttggccagga actcgctctt ctcgcgggtg gcgttctctg cgtcgcggac
     1681 ccgctgttcc agctcccggt tcaggtcgtt gatggttcgg gaacgctctt cgacgcgctc
     1741 ttccaggtga ttgcgatagc gctccagttc catttcggtc cgtcggcgct cgatggcgat
     1801 ggaggccagg gaggaggcct gcccgatgag ggcgaaatct tcttcggtcg gcgccgaggg
     1861 gtggcggtga tagatggcga aggtgcccag cactttctgg tgggaatcgc ggatgggctc
     1921 ggaccagcag gacgcgaggc cggcgtgggc cgcgagttcc ttgtagggcg cccagtaggg
     1981 gtgggtggcg atgtcctcca cgatgacccg gctcccggtg gccgcggcgg tgccgcagga
     2041 gccacggccg aagccgatct cgagggtttc gatggccttg ttgtagaagt ccggcaggcg
     2101 cggggccgcg cccttgagca gatgccggcc ggtatcgtcg aggagcagga tgctggtcag
     2161 ggcggtgggg tcgatgcgct cgatgcccac gacaatcgct tcgagtaccg tggcgagggg
     2221 ggccgcatcc acgaggaact gcaggacggt gctgcggtgc ttttcgagat tctgggaaag
     2281 cttctgggcg gagatgtcgc ggatgatgat ggagagcaga gtcggcgcgc cggacacgtc
     2341 ccgctggggc aggatctcct gccacacggg cacttcgcgc ccggtgggcc gcaggatggc
     2401 gctttcgcct tcccagtgcc cggtcgccag cgcggtggga atggcggtgt cccgcagcag
     2461 ctgcgcggcc cagggcggat gcaccgtggg gaggctcagc gccgtcagat cggcatcgtc
     2521 cggccggccc agcaggcggc aggcggcctt gttgtggtac ttcaggtggc cgtcgagatc
     2581 gatgacgccg atgaagtcgg gcgaatcggc gatgatcccc agcaggtttt cggtattcgg
     2641 ctccaccgcg acttctccgg aaattggctg atgcctggtg atgctaaagc ctgcctgcct
     2701 gtttcggatc tgttggaatc aacagggcgc aacatcatcc cgggcagtgc aagcctattg
     2761 ctgcgctata gcggtccgtg cgtcaaaaga tcaagggcgt gtcgggcagg ggcgggacta
     2821 ggtcattgtt tgatatgagt gtagcccacc gcgcgcccga cccgcgcatc caatccgcgt
     2881 gccgcgacgg gcgggcgagg ccggacgcag ggcggcgttc ccggtgcgcg cggatgaatt
     2941 ctgcgcgggg cgacgcgggg tcggttcatg ctttcgggcg ggcttcatga acgatatcgg
     3001 ccgtggcatc gacagctgaa cgcgcatcgt caacgcgggt gtagggcgat atcgcgggcc
     3061 ggcgcggcgc aaggcacagg ccggttcttc gggtgcgggg gcggtgccat cccatggggg
     3121 catgacgacc ggaggcgatg ggggacgcca gaccgcaggc gggcggtccg gcgtgatggc
     3181 ggggcttcag gtgaggttga agtgggcgtc cggctggggt ctgggcggca gcgggtgatc
     3241 gtcgttgagt tccttgagct ggatctcgat ggtggtgcac agggcgttga ggggcaggtc
     3301 gttgggggcg gtgccgaagg ggtcttccag ttcttcggca atcgattcga gggacaggaa
     3361 ggtgtaggag atgaagaccg acacgacggg cgtcatggcg tgcaggtcgg acaccagggc
     3421 gaagggcagc agggtgcaga agaggtacac cgtgcggtgg aggatgaggc tgtaggcaaa
     3481 gggaatgggc gtgctggcga ggcgttcgag gccgccgagg atgcgcgaga gttcgttgag
     3541 gttggcgtcg aagctctggt agaggacgtc gctgatctgc ccggcgcggc gcctgtcgcc
     3601 cagccacagg tccaggtgca ggaggatgcg gtgggcggtg agggggctgg cgctcacgtc
     3661 ggccaggtag cgctcgggga gcaggcggcg caggtcgtcc tgggggctgc tgccgcgcag
     3721 ctggtgcttg aggctccagg tgaaggcgag cagcagaccg gcgaattcgc gggcgctggc
     3781 ggcgtcgggc agggcgctct tccactggcg cagcagagaa cgggtggtga ccagcaggct
     3841 gccccagaag aggcgggctt cacagtagcg gccgtagctg gtgttgttgc ggaagcccag
     3901 gaagatggcg atggccacgc cgaggaggct gaagggggcg gtggtgaggt ggatgtcgag
     3961 ctgttcgtac cagggcaggg cgatgaccgc gacgatgccc atcatgacgt tgaggctgag
     4021 gcggaacagg atgttgggga gcacggagcc gtgccagtcg aagagtcggc ggaaccagtg
     4081 ctggtggggg cggacgatca tggggtggac aaaaaggaca gggctccggc gggatgcggc
     4141 gttctgcgac gcctgccgcg acggggcggg gtggatcggg cgagtggggc ggcctgcccg
     4201 gcgaggggcg cggcgtcggg gcgtattgtg gcctgaagtg gccgtcttga gggagaggcg
     4261 gcccctgtgg tcgggccgca ggatgagcgg gggcccccag cgcaggtcgt ggccggtccc
     4321 gcgcagaccg cggggccggg agatgccggg gcggtgcgtt cagccggcgc tgcgcgctgc
     4381 gtagcggttg gcgagcaggg agcagacgaa gagctggagc tggtggtaca gcatgatggg
     4441 cagcaggatc agcccgatgg aaggatgggc gccgaagagc agcttggcca tgggcacgcc
     4501 ggaggcgagg gtcttcttgg agccgcagaa gacggtggcg atctcgtctt cgaccgggaa
     4561 ggcgcagcgc cgcaccagcc aggtggagaa ggcgaggatg aaggcgagga aggcgccggc
     4621 gccgatgaag gcgatgccca gggatgcgag cccgttgtgc tgccacaggc cggaggcgac
     4681 cgagtcgcag aaggcgctgt acaccaacag gaggatcacc cagcggtcca gcagggtggt
     4741 gaggcgcttg tggcgggtgt gccaggcgtg cagcagggga cgcatgagct ggccggccac
     4801 caggggcagc aggagcaggc gggcgatgcc gatcaccgac gccatgaagg gcaggccgtc
     4861 gccctgcacg ccggtgaagg cggccaccag caggggcgtg gcgacgatgc ccagcaggct
     4921 ggagagggtc gcattgaaga tggcggcggg gacgttgccg cgggcgaggc cggtcatggc
     4981 caccgacgag gagatggtgg agggcagggc gcacaggtag cagaagccga gggcgaggcc
     5041 ggcgggcagc cagcggccaa gcagggcgta ggcggcaagg cccacgaggg ggaagacgac
     5101 gaaggtgcac agttgcacga cgaggtgcac cttccagcgg cccaggccgt tgcggaaggc
     5161 ggtcagggag atgccgaggc cgtggaggaa gaagacgagg gcgatgccgg cgtcggcgac
     5221 gcggtcgcca tggagaacgc cgccgctgcg gcccaggttg ggggcgaggc tggcgagcag
     5281 cacggcggcg atcatgccga cgaggaacca ctccttgtgg agggtgcgca ggagtctggg
     5341 cagggcgggg ggcgtcatgg gcgtctcctc tggcttgcga tgcgggggga tgaggtggaa
     5401 cgatacgggc cttcggtctg gccggctaac ggcatcaggc cattccatgc cgcgaaactg
     5461 acatctccag gatgtcgaga ggtgaatcgg tgaaacgtat cctgcccgcg ctggcggctt
     5521 tgccccgtcc ggtgttcggc cgcaatgaat ccctggcgcc ggggatgtgg acggacgtcc
     5581 atacccatcc ctggtgccag ctttcatacg cggtgagcgg cgtgctggcg gtggcgacgc
     5641 ctcaggggga ttacgtggcg ccgccccact acgcggtgtg gattccgccg gaggtgccgc
     5701 accaggtgtt caaccggggc cgcacggaga tgcgcagtct gtacctggag aacggcgcgc
     5761 cggggctgcc gacggcctgt gcggtgctgg aggtggcgcc gctgctgcgt gagctgatcc
     5821 tgaaggtggg cgcgctgccc ccggactacg tgccggacgg ccccgccggg cggctggtgg
     5881 cggtgttgcg ggatgaactc gccgccgcgc ggcaggcggg ctttgcgctg cccctggcga
     5941 aggaccggcg tctgctgccg atccaggcgg cgctgcagga caacccggcg gatgcccgca
     6001 cgctggccga atgggccacg caggtggggg cgtcggagcg caccctggcg cggtgcttcg
     6061 tcaaggagac ggggctgggc tttggcgact ggcggcggcg cctgcgcctg ctgctgtccc
     6121 tgtcggcgct ggaggcgggg gacagcgtga cgacggtggc gctggccgca ggctatgact
     6181 ccacgtcggc cttcattgcg gccttccgcg aggtcttcgg ctttacgccg ggcagtctgc
     6241 cggggcggag ctgaacgcgg cgttcaggcg ccgaggaggt ccagaaaggc ctgggccggc
     6301 atcgggcggc cgaagtggta gccctgggcc aggtcgcagc cgagcaggcg caggtcgtgg
     6361 gcgatgtcgg cgctctcgac cccttcggcg aggacggtca tcttgaggct cttggccatc
     6421 tggatgacgg cgcggacgat ggcgccgtct tccgggtcgg tgggcaggtc gcggatgaag
     6481 cactggtcga tcttgagctt ctccacggcg aagcggcgca ggtaggcgag gctggagtaa
     6541 ccggagccga agtcgtcgat ggacaggcgc aggcccatct gacgcagctg gcggacgacg
     6601 gcgaggacct gctcggtgtc gtggagcagc accgattcgg tgatctcgag ttcgatgcgg
     6661 tgggggtcgg cgccggtgtc ggcgaggagc gcgcgaacgg tgtcgaccag atcgccgcgg
     6721 gtgatctgca agggcgagag gttgatggcc atgaccagct gatggccggc gtcctgccag
     6781 gcgcgggcct gggtgaaggc ttcgcgcagg acccaggcgc caatggggac gatgaggccg
     6841 gaatcctcgg cgacggggat gaagtggccg ggcggcacca gggggcggtc gggcggctgc
     6901 cagcgcagca gggcttcggc gccgatgatg cggttgtcgg caagatccac cagcggctgg
     6961 tagtggagga tgaactcgtt gcgctccagg ccgcggcgca ggcctttttg caggtcgagg
     7021 cgcgcgaggg cgtcctcgtt catgcgctgg gagtagaagc ggaaggtggc gcgcccggcg
     7081 gccttggctt cgtacatggc ggcgtcggcg tgcttggcga gggtgtcgaa gtcggtgccg
     7141 tcgtcgggga agacggcgag gccgacggag caggtgcaca gcagctcgtg gccgtcggcg
     7201 tggtagggct cggcgagggc ctgcatgatc ttctcgacga cgggggcgat gtcttcggtg
     7261 tcgcggatgc cgttgaggag cacgagaaat tcgtcgccgc cgaggcgccc gaccaggtcg
     7321 gattcgcggg tggcgctgcg caggcgttcg gcgacggcct gcagcagacg gtccccgatc
     7381 gggtggccaa gggagtcgat gatgtccttg aagcggtcca ggtcgaggaa cagcagcgcg
     7441 ggtttggcgc ggttgcgttc ggcctggacg aagcactgct gggcgcgctc cttcaggagg
     7501 aggcggttgg gcaggcaggt gagggcgtcg tggtgggcga ggaattcgat gcgcgcttcg
     7561 gcggccttgc ggtcggacag gtcggagaag atggcgacgt agtggaggag cttgccggcc
     7621 gggtcgcgca ccgcggagag ggacagccac tggggaaagt agttgccgtt cttgcggcgg
     7681 ttccagactt cgccgctcca gtggtcgtcg ccggcgaggg cgtcccacag ctggctgtaa
     7741 aaggcgatgg agtggcgctg ggcggagagg agctgggggc cctggccgat gacttcttcg
     7801 ggctggtagc cggtgagctg ggtgaaggcc gggttgaccg agatgatccg gttgtcgtgg
     7861 tccgtgatca tgatggcctc gcgggtggcg gcgaggacgg tggcggccag ctgaagctgg
     7921 acgcgctggg cgcgttcgcg gctgatgtcg cgggtggtgc cctcgacgcc gacggtgtcg
     7981 tcgtccgggt tgcggtagag gtgggcactg gtggcgaccc agacctcgtg gccgtccttg
     8041 tggcgcaggc tggcttcgta atgttcgacg gcgccgccgg cttcttcgag ggtctgcagg
     8101 aagacgtcgc gctcggtggg gcgggtgtag aggcgggcga tgggggtgcc gatgagctct
     8161 tccgcgtcgt agccgaggag cttgcggacg gaaggggaga cgcgtacgag gcagccgtcg
     8221 gcgtcggtgc ggtagtaggt gtcctgcagg ttgtcgagga cggcgcgcag ttcggcttcg
     8281 gactgggcga cgcgggcgcg ggcggcgtag gcggggccga ggtcggtgat gaagccgagg
     8341 gtgccgacga tctcgtcgcg gacgttgcga tgggtgacgg tgctgatcag ggccgggaag
     8401 gtgctgccgt cggggcgcag gccttccagt tcatacacgc gtcgctgggt ggtggcgacg
     8461 cgaccgatct ggcgggtgag ctcggggcgg ctggcggggg tgacgaagtc aagcgggctg
     8521 cgtccgatga aggtctcccg cgggcagccg aagagggtgc agaaggcctg attgacgtcg
     8581 aggaagcgat agcgggcatc gaccgtgaag aagccgtcgg cggcgctggc gatgatgttc
     8641 cggtacagcg cgtcgcggga gagaagggct tcctcgatgg tggccggtgc gtcgtggggc
     8701 ggcgacatgg tgatgacgga cgtggacaac gtccgtcatt acggccgcgc ccggccggac
     8761 ttgaggtccg gccggacgtg tcaggcgccg atgccgaggc gttcgcagag ggccagggtg
     8821 gggccggact ggttcatgca gtagaagtgc aggccgggag cgccggcggc gaggagcttt
     8881 tcgcacaggc cggtgacgac atcgaggccg aaggcgcgga tggagtcgct gtcgtcaccg
     8941 aaggattcga acttgcggcg catccagcgc gggatctcgg cgccgcaggc gtcggagaag
     9001 cgggccagct tggagaagga gacgatgggc atgatgcccg gcacgatggg gacggtgatg
     9061 cccagtttgt cgcattcgtc gcggaagtgc aggtaggcgt ccgggttgta gaaatactgg
     9121 gtgatggcgg agtcggcgcc ggcggcgacc ttgcgctgga aggcgaggag gtcgtcgcgg
     9181 gggctgcggg cctgggggtg gtattccggg taggcggcga cttcgatgtg gaagtggtcg
     9241 ccggtttcgg cgcggatgaa ttcgaccagc tcgttggcgt agcgcagctc gccggcaaag
     9301 ccgacgccgg agggcaggtc gccgcgcagg gcgacgatgc gacggatgcc ggcgtcgcgg
     9361 tagcggtgca ggatctcgcg gatgtgctca cgcgtggagc caatgcagga cagatgcggc
     9421 gcggcttcgt ggccttcgct gcggatctcg aagacggtgt cgaaggtgcg gtcctgggtg
     9481 gtgccgccgg cgccgaaggt gacggagaag aactcgggct tgagcaccgc cagcttctgc
     9541 cgcacgacgc gcagcttgtc ggcgccttcg ggggtctggg gagggaagaa ttcgatggag
     9601 aggttggggg tcatggcgga tttccggagc aagacgggcg accgtccggg cgccggcagc
     9661 gggcgcgcgg acggtgacgg ggccggtcag tagcggtagt gggcggactt gtaggggccg
     9721 tccaccggca caccgatgta ctgggcctgg gcgtcggtga gcgtggtcag cgcgacgttg
     9781 agggtcttga gctgcagacg ggcgaccttt tcgtcgagat gcttgggcag ggtgtagacg
     9841 cccaccgggt agtcggcggt cttggtgaag agctcgatct gggcgatggt ctggttggcg
     9901 aacgaggaac tcatcacgta gctggggtgg ccggtggcgc agcccaggtt caccaggcgg
     9961 cccttggcca gcaggatgat gcggtggccg gagggcaggg tgatgtggtc gacctggggc
    10021 ttgatctctt cccaggggta ggcttcgagg gaggcgacgt tgatctcgga atcgaagtgg
    10081 ccgatgttgc agacaatggc gttgtgcttc atcgcgacca ggtggtggtg gtcgatgacg
    10141 tggtagttgc cggtggcggt gacgaagatg tcggccttgt cggcggcgta gtccatggtg
    10201 acgacgcggt agccttccat ggcggcctgc agggcgcaga tggggtcgat ctcggtgatc
    10261 cagacctggg cagagagggc gcgcagggcc tgggcggaac ccttgcccac gtcgccatag
    10321 ccacagacga cggcgatctt gccggcaacc atgacgtcag tggcgcgctt gatgccgtca
    10381 acgagggatt cgcggcagcc gtagaggttg tcgaacttgg acttggtgac ggaatcgttg
    10441 acgttgatgg cggggaactt gagttcgccg cgctcgtgca tctggtacag gcgatgcacg
    10501 ccggtggtgg tctcttcggt gacgccctgg atccgggcga ggcgggtgga gtaccaggtg
    10561 gggtcggtct tcagcttggc cttgatggcg gcgaagagga cggtggcttc ttcgctgtcg
    10621 ggcgagtcga ggaccgacag atcctgctcg gcgcgggcgc ccagatgcag cagcagcgtc
    10681 gcgtcgccgc cgtcgtcgag gatcatgttg gagtaagcgc cgtcggccca ttcgaagatg
    10741 cggtgggtgt agtcccagta atcggtgagg gattcgccct tgacggcgaa aacggggatg
    10801 cgtgcggcgg cgatggcggc ggcggcgtgg tcctgggtgg agaagatgtt gcaggaggcc
    10861 cagcgcactt cggcgccgag ggcgatgagg gtttcgatga gcacggcggt ctggatggtc
    10921 atgtgcagac tgccggtgat gcgggcgccc tggaggggcc gggtcttggc gaattcgtcg
    10981 cggatggcca tcaggccggg catttcggtt tcggcgatcg tgatctcctt gcgaccccag
    11041 tcggcaaggg acagatcggc gacgacgcaa tcggtgaagg tttcagccac agcgttcatg
    11101 agaactcctc gaacatgtgg ccgggaaggg gatggtacgg gtgggctcac cacgtctccc
    11161 cgtgcccggc acgggtttgc acacaagcat ggacgggtga gcgcggttgc aggtacgtcg
    11221 cggcgggcgc cgcgggcact gacgtcgagc ctgggacggc ggcggcgtgg gccgggtcgt
    11281 cttgcagcgc tcctcgaaac aggtcgcgga ttatatcggg ttttgcggcg gtggcttgag
    11341 ctgcgtgcgc tcagtcggcc tgtccggcca tgggcggcag acgtccgagg cgcgcggtga
    11401 gggcccgcag ggtgggctgg ccggtctttt cgaggacgcg ccgcacgtgg gtgcgggcgg
    11461 tgttgggggt gcagccgatg cgcgtggcca cgtcgtcgac gctggcgccg ttgtacaggg
    11521 cgccgaggat gcgctcttcg gcccgggtga ggccatgggc gcgggcgtag aagtacaggg
    11581 acaggccggc cgtctgggtg gattgctcga agacgatggc ggccggtgtg ccgctgtcgg
    11641 tcaccatgcc ggtgaggggc aggatggcca cgtcgacgct gtgttcgccg tgcccgaggg
    11701 agaggtaggt gcgcaggccg ctctgcacgt cgtcgagggc gtcctgcagc aggagggcgt
    11761 cacgggcgtt gggcgcttcg accaggtcgt ccaggatctg gagcacgccg cctgggtgga
    11821 agtgggcgag cgcggtacgg ctggcgtaga tgagggtgcg atcccgcgcg aggacggcga
    11881 tgccgaaggc cagctcgtcg agcagaagtc ccatggacag atcccggagc agatcgctgc
    11941 gggccggcgg agtgtcgggg gcggcaaacg gtgcggtggg catggcaatc ctcaagagcg
    12001 gaggggccgg cgggcgcggg tggggccggc ggtcattcct gcatatgcta gtggcgcatg
    12061 cctgcactgc aaaacgatgt cacctaatgg gggtcaatag gcttcatgaa cggtctgggc
    12121 gtcggcgtgg ggcgagtcac ggcggcgcag atgcgtcggg gccaggcgat gccggtggag
    12181 gggcaatggg cgggcggtag cacacgtgca gggtctcgtc ctcgtggaaa tcgaggcctg
    12241 aaccaggctc tgtgagggcg gcgacggtga cgtcgcgggc ggcgtggagg tagatcgtgt
    12301 ccggcgcgcc cggcaaccgg gcgtcgcgca gcgcctggcg gatctgtagg gcctgcgcgg
    12361 tggcgggctg ggggctgtga aaccacacga ccagacagcc gtccgtgtag tgggcaaccc
    12421 actggcggat cggggcgatg ccggccagac agggggcgca cagatcgcgc atccagctgt
    12481 cggtgacggg ggcgtcctgc cacgcgagca tgaaggtcca gacctggcct tcgttccagt
    12541 ctccatcccg gtgcgccggt ccgcagtagg cctgcccggg gacgctggca tcgcccggtt
    12601 gccagcgctc cggccagcgc gccgcgaggg cgtcgaagta gaggccgtgg gaggcggcgt
    12661 agaggcggcc gctgggggtc cggcgggcaa tgtcgtatgc gtcgcgggag gagtagaaga
    12721 ccagcgcggt ctcttcggga aggtccgggt aggcatccac caccggtggc agcgtgggaa
    12781 agtaggcgag cagtccaagg ggcgccatga gctgcggtgt cgccggtacg aaggtctggc
    12841 gcagggcggt gagaaaggct tcgcggctca gggcggggcc ggtgatgttg tcaagggcgt
    12901 cgcggttctg acgtcggcgg gcgatccaca cccgcaccgc cagcgcttcc ggttggggcg
    12961 gctcgggtga tgcggccatg gggatgctca gttcaggaag tcggcaggta ccggctggag
    13021 gtcggcgtgc atctcgagga accagcggat gacacggcgc acgcgggcgg gcaggcgtcg
    13081 ttcggccagg cggcagatgg agatctgctg ggtgaccggc gggtcgttgt cccgatggcg
    13141 gatgcgcacg aggcggccag cggcgagatc gtcggccacc acgtgcaggg gcaggaccgt
    13201 ggctcccatg ccgcccttga ccatcgcgat ggcgccgcgc aggtcgctga attcgccgcg
    13261 gacggacaga tcgtcggcgt ccgggctcat ttcccggcgg gccagaccag acagccagcg
    13321 atgcaggtag aaggcgctct cggggaagca cacgtagggc aggcggacga tgtcgtccag
    13381 gtgggagagg gggcgggggt ggagtgaagg ggccgcgacc agaccgtatt cctcgacgca
    13441 gaaggggtgg tggatgagct gcttttcgtg gcgcttttcg aggacgaagc cgaagtccac
    13501 ctcgccgccg agcacgtcgc ggctgaccgc gtcggtgggt ttgagggtga cgcgcaggtg
    13561 gataccggga aattcgaggc ggcgcgcgag cagctgggga aaatggggcg aatggatgca
    13621 gctctccggc atcgaatagg cgacgaggcc ctggatcgat tcgaactcgg cgttgaggcg
    13681 ctccaggaag gaactggagg actgcaggta gctttcggca aattcggcca gcagctgggc
    13741 ggcatcggtg gggaggatgc ggccgttggc gcggacgaag agctgacatt tgaggcgctc
    13801 ttcgaggcgg gcgatctgct ggctcaccgc gccctgggtg agggatgcaa tgcgcgcggc
    13861 ttcactgacg ctgccgctgc ggacgacggc caggaatccc cggaagagtt tggggtcgag
    13921 tcgttcaaaa tcgcttgccg tccaggacat tttggctccg tctgggcgat tgttcaggaa
    13981 atatcgttat tcaggcatat acggagaagg tatcacaatt cggtattggc aaaatcggca
    14041 aattcgaaaa tgcagccctc agggaccgcg aatgccgggc aaaagtggca gggaccggcg
    14101 ctgcgcagga ggcgcgcgtt cgcggcgcgg tcgggggcgc gttcgggcag atcgccgctg
    14161 tggcgcagct gggcgatgag gcttgcaagc gcttcgatgc gcgtgtcgcc accgatctcc
    14221 acgaggcggt agagggcgtc gccttcatcg agctggtcga tggtctgcca cgtgtcggag
    14281 agcaggcgga agcttggcgt ggcggtgtcg aggagggcgt gcgcgcccgt accggagttt
    14341 tccggcgcct gggcgatcag cgcgaagctt gccgggcagt gctcggcaaa gcgcgaccac
    14401 aggggcgagg cgacccctgc gtcgaaggcg atcggacgga tcgggtagga cagatgggga
    14461 acggcgacca catgggcgcg ccaggggggc aggtcgccgt cggccgggag atggagggcg
    14521 aggcggcggg tgagcgcgcc ggtgtcgagt tcggtcttca gcccggccag caggcgggtg
    14581 acggtgacgt ccggccccag tgcggggaac gccggcaggc cgacggcgcg caagaacagg
    14641 ccgagggctt ccaggcccga gaagatcagc aggcgggggg ccgactggcg gatctggcgc
    14701 agggcgtggc ggcggctgtc gatgcacgcg gcgctcagtt cgtcccggtc gacgccggtc
    14761 ttgtcgctcc agccctgggt ggcgcaggcc accatgtcgt gcagggaaat gtcctcgccc
    14821 ggcaccagat cgctgcgccg gcagatctcc tgggcccgcg cgtagtagct gctgcccacg
    14881 tcggcgcgca ccgcgatcgt ccggccttgc accgaggcgg cggtcatcac gccgccgatg
    14941 cgttcgccgg cggcgaagcg cgcgcgcagg gtcacgtagt gttcatcggg tagccagtcg
    15001 aactgcgtgg tgtcggtgcc gccgtcgtcg aactgcaggc cgagggtgac gtggcgggcg
    15061 tggctctggc gaccggtggg gctggcagcc gggtgtttct tgtccaccgg aatcacgctg
    15121 ccggccccgg cggcccgcaa gtcgtgacgg gtggcgtcga tgagcgtctc cacgtcctcc
    15181 gggcgcatgc ggtattcgtg gtgatccggg gtcacatagc ggtgggcgcg ggcgtattcg
    15241 gcgaggctgc ggtgccgcgc atagacggtg ctggggccct tgagggcgta tcgtggggcg
    15301 tcgggcaggg tccagaagcc ggtgaggttg ggattgatgc cgacgatcat caccggggcc
    15361 ttgcggcatc cgcgcagggc cgcgggatcg ggcaggccga agccttccac ggtgacggcc
    15421 tgcggggtgg gggcgggcgc atcggcgcgg gctgcgggga gggcgtccag ggccgcgccg
    15481 tcacacaggg gaaaggggcc gaaacggtct ggggcgcgcc acagggatgt gcagtcgcgg
    15541 cagctgcgga cctcatccat cagggcgact tcgataggcg gcttcatggc tggctccagg
    15601 cggggatgag cacggtggcg gtggtgaagc tgaccctgtt gcggggggcg ggccgtccgt
    15661 ccttgaccca gtgtttggcg cccgcccgca ggtggatgcg gcgcggcagg tcggggatgt
    15721 ggagggcccc cgaacagcgc cacgccgact cgccgcccag gcggaagtag ctggcccaga
    15781 attcaccgca gcgcaccagg cggagttcgc cggaaatggg gcccttgagc gggccgccga
    15841 cgtcctctcc ataacggttg tagtgattgg aggacgccgt gacgctgtcg tcgatgcggg
    15901 ccagcgcgcc ggccaggttc cagccgatgc ggaagccgtc gttctcgtcg cattcgctgc
    15961 tcacgtaagg cggggcgcca tgcacgtcga agcacaggct gcggtatttg tcgtcggccg
    16021 tgtggctgcg gtccacgagg gagcgccgcg gcggatcgat ggtgagggcg gccagttcca
    16081 gcgtggtgcc ctgggccggg tcctcgctga cgaaggtgat gcgggcgtcg aagtcggtga
    16141 ccagggcgcg tgcgaggacg gcggcgccac cggaatagcc ggtgccggcc ggcatgtcca
    16201 cgatgaaggc gggcgcctcg ggcggtccga acaggatgaa ggactccggc acggggttgg
    16261 agtagccgaa gatccagcgg cggctggcgg cgccgtccgt gaagtcgtcg tccagcagga
    16321 tggcgctttg ctgggcgcgg gcgtccagat cggcatccgt cgtcagccag acgtcgggct
    16381 ggaactggcc ggagcggccc cgtcgcacga gaaggtaggc gccgtcggag cgcccgccgg
    16441 ggaaggggag atccgggaag cggtgtgcgg cgctgtcgct gctccaggcc tggtggtcgt
    16501 ctgcactggg ttcggcatag gggttgaggc tgcaggcggt gtcgaagccg gtcgcgatca
    16561 cgtcggcgag catcgtggcc ggtacgatca ggcgcggcgg gtggcgcagg gcggcgccca
    16621 gcaggcgacg ggcgcaggcc tgcacctgat cgtgcgtgag gggggtctcg tggggcacca
    16681 ggaaggcgag ggggcaggcc tgaccgaagg tccacaggaa atcgtcgagg gacagggacg
    16741 gcacgccgtc aaggcccgtc ggccccgcgc attcgaggcg accgtcgacg ctgtgcagga
    16801 tggtggcaag gccgctcgcg ccgagggcaa gggccgacca ccacggatcg gtgccggtgc
    16861 cggggcggcc ggccccgtgc cgagtgtcgc caccgatggc aaggcaggga agggagggtg
    16921 tgggcatcgc ggggcgtctc cggaggcgcg aaaggaaatc ctgcctgtgc acaggcgccc
    16981 ttcggcgacg gaggccgaca cccggtctgc gcagaaggcg gcgtggcgca tcggcgcagg
    17041 gcccggagat gggcgtgctg cataggcagg cgcccattct gggcgcctgc ctatgcgttt
    17101 gtcgtcatca gaattggtga atgcgcccga tggcccacga cggtccgggc cgggggctca
    17161 ggggagtgcg gcgtcgaggg tccgcaggaa ggcgtcggcg gcctggaagc cgatgacacg
    17221 ctgggcgcgg cgttctgcgc cgtgggcgtc gaaaaagagg atgcccggcg ggccgaagag
    17281 atcgaagcgc ttgagcaggg ccttgtcgtc ctcgctgttg gcggtgacgt cggcctgcag
    17341 gaggaggacg ccggcgaggc gtcgggcgac ggcggggtcg gcgaaggtga agcgttccat
    17401 ctccttgcag gacacacacc agtccgcgta gaagtcgagc atcaccgggc ggtcggcggt
    17461 cttgaggatc tcgtccagct cggcgccaga ggcgatgcgg cggaagtgcg ggccctcggc
    17521 cggcgcggcg gcctgggcgc gcaggatg
//
LOCUS       DAOQMM010000079        17541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1221_length_17541_cov_43.982214, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000079 DAOQMM010000000
VERSION     DAOQMM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17541
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_17541_cov_43.982214"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /gene="dsbD"
                     /locus_tag="PLH85_12415"
     CDS             complement(<1..1497)
                     /gene="dsbD"
                     /locus_tag="PLH85_12415"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNB65416.1"
                     /translation="MNRALLRLFALLLPALLMLGLLVARPAAAAEPLPAEQAFHLDAR
                     ALDDRTVEIRFQVAEGYYLYKHKFKFEADGVTFGEAQLPPGKRKKDDIYGEVETYRGE
                     LVFPLPLSAGQPPFKLTVTSQGCADLGICYPPTPQTVSIDPAGQRKVGGFLARALGQA
                     PAAEAPLTAPASTTAVPADAHDESGRIARLLAGGGTLATLASFFGFGLLLAFTPCVFP
                     MIPILSGIIVGHGHAISRGRAFTLSLLYVLGMALTYTAAGVAAGLSGTLLSAALQNPW
                     VLGGFALVFVALSLSMFGFYELQLPTALQSRLSDRANHQPGGSPAGVILMGALSALIV
                     GPCVAAPLAGALLYIARSGDALLGGAALFTMALGMGAPLLLVGVAARSALPRPGPWME
                     GVKRAFGVMLLGVAVWLVSPVLPAVVPMLAWAGLLIMSAIYLHALDPLPPHAAGWQRF
                     WKGIGVVALLTGAALFIGALAGSRDPLQPLAILRAQAAAPAEGPHFRRI"
     gene            complement(1544..1876)
                     /gene="cutA"
                     /locus_tag="PLH85_12420"
     CDS             complement(1544..1876)
                     /gene="cutA"
                     /locus_tag="PLH85_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNB65417.1"
                     /translation="MADPDILLVFTTLPDADSAARIARQLVDARLAACVSIQAPCTSV
                     YRWQGQTETATEVPLLIKTPRARYAALQETLRAAHPYELPEIVAVPVSEGLPAYLAWV
                     AGETAPAS"
     gene            complement(1886..2671)
                     /locus_tag="PLH85_12425"
     CDS             complement(1886..2671)
                     /locus_tag="PLH85_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB65418.1"
                     /translation="MNPPQPAPPRLSGRIAAVWRRNFLVWRKLALPSIVGNLADPVIY
                     MLGLGYGLGMLVPEVGGQSYIAFLAGGTLCYSTMNAATFEVLYSSFSRMHVQKTWDAI
                     LNAPVSLDDIVAAELLWAASKATMAGLAILAVIALFGYTASPLALAALPVVFLTGLAF
                     AALGLIMTALAPGYDFFTYYFTLFITPMTLLSGVFFPRDQLPGGFQLLAELLPLSHAV
                     ALARPLLQGHWPVHAGLHLVVLTACAGIAFAVARVLMRRRLLK"
     gene            complement(2668..4008)
                     /locus_tag="PLH85_12430"
     CDS             complement(2668..4008)
                     /locus_tag="PLH85_12430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB65419.1"
                     /translation="MSACPDPRTIIEHLPVALAVARATDGRLLSSNRGFAALFSAPAT
                     LGMPLTDWLRHADAGDRIGPPPDWPAHLAALHPDIRPHDSVECLLRGPAGPLHIRVQL
                     QVADGLLIACFVDLTDHETREDILRQALAELETRSATDALTGLLTRRRFEEAAASEMQ
                     RFRRFGHPITLIMADIDHFKLINDRLGHQAGDRVLVEFARRILAECRDLDVIGRYGGE
                     EFLVLLPSTPLVGGLTLAAKLRQAIAAAPFPDAGPLSASFGVAECQADEDWHAWLHRT
                     DKALYRAKRQGRNLVVAAPERHDQPHAHAELSRLDRLGWEDGHTLGHPLMDHQHKELF
                     TLAQALLEMIRRDGPTADLHVAIRELAHTTERHFRDEEALLGEIAYPRTDQHMKAHRL
                     LAAKLDALSSQSARGVLPAARLIEFLAYDLVDHHALGPDRDYLPWLDGRTPETP"
     gene            complement(4105..5043)
                     /locus_tag="PLH85_12435"
     CDS             complement(4105..5043)
                     /locus_tag="PLH85_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB65420.1"
                     /translation="MPLEIAGLVKRFGHGADAREVVRGLDLSLRPGECFALLGPNGAG
                     KTTTLRCALGLTAASAGSIRLCGLPVPAQAREARARVGVVPQLDSLDPDFTCAENLLV
                     FAGYFGIPDSEIRPRIPELLAFAGLEGKENARIPSLSGGMKRRLTLARALVNRPELLI
                     LDEPTTGLDPQARHLIWERLRRLVAEGTTVLLTTHFMDEAERLADRLAILDGGRILAQ
                     GSPREVIAAHIEPQVVEVFGDWADAPGGAPAWASRHAAALARRCEVSGETAFCYTDDA
                     APLLAHLAHQSGLRTLHRPANLEDVFLKLTGRELRD"
     gene            complement(5043..5651)
                     /locus_tag="PLH85_12440"
     CDS             complement(5043..5651)
                     /locus_tag="PLH85_12440"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNB65421.1"
                     /translation="MKLIVSLTSPFARKIRVILAEKGLPCDLQVDIPWLAETNVPDYN
                     PLGKVPVLVADDGEVWFDSPVIAEYLETLGGPALLPADRIASLPVRQSEALADGITDA
                     AVSAFLEMRRPAPQQDPASLERQITKIRRGLGALEERFAARKGFGGDTLTLADIAVGC
                     ALGYLDLRFAHLDWRAGRPALAAWAAGLLARPSFVASVPPAV"
     gene            complement(5680..6327)
                     /locus_tag="PLH85_12445"
     CDS             complement(5680..6327)
                     /locus_tag="PLH85_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB65422.1"
                     /translation="MNRPAATESALINGPAGAIELLIDTPADVRGIALVCHPHPLFHG
                     ANTNKVAHTLARVLRDLGYAVVRPNFRGVGKSAGTHDNGHAETDDMLAVLSWAESRWG
                     SLPIALGGFSFGAYVQTRVAARLAESVAPAQRIVLVGTAAGYVTGARSYTTLPVPADT
                     LVIHGEKDETVPLANVMEWAEPQEIPVVVIPGADHFFHGRLHILRTVIERAWTPL"
     gene            complement(6334..6642)
                     /locus_tag="PLH85_12450"
     CDS             complement(6334..6642)
                     /locus_tag="PLH85_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNB65423.1"
                     /translation="MSYFKHHVFICCNQRPEGETCCNARGGSELQAYAKDRIAALGLK
                     GRGKVRINKAGCLDRCDEGPVLVVYPDEVWYTFVDKADIDEIIHEHLQHGRIVERLKI
                     "
     gene            complement(6708..7763)
                     /locus_tag="PLH85_12455"
     CDS             complement(6708..7763)
                     /locus_tag="PLH85_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNB65424.1"
                     /translation="MALHFAVAIGVLIVYACLHPFTGWRDPGLPLLDFLTAPWPRYYS
                     QTDLIVNVLGFVPFSFALVPALGGRLRPGGAVCMAVVIAGLLSLGLETLQNFLPSRVP
                     SNVDLGCNALGGLIGALAGARWGAPLFDTRGALARWRTRRIVAGHVGEAGMVLMGLWL
                     LTLLTPESLLFATGDLRRLFDLPTPIPFNPQRFVKVEAAIAASQLVAVGLTARCMMRS
                     PSPFPILLLVLLGLGAKSLAAGMFFSPGDPFHWATPGGQIGIAAGAALLTACLILPTR
                     LHAVLAGLALLVGTALVNLAPENPFLPNDTLLALRGNLLNFHGLTSLAASLWPFLAFL
                     YLGLLGGRSGRDGAGPH"
     gene            complement(7923..10670)
                     /gene="ppc"
                     /locus_tag="PLH85_12460"
     CDS             complement(7923..10670)
                     /gene="ppc"
                     /locus_tag="PLH85_12460"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNB65425.1"
                     /translation="MSEDKDLPLRDDIRLLGRVLGDTLRAQEGEAVFDLVETIRQSSI
                     RFRRHADPAARKELEATLDSLSRDQTIDVVRAFSYFSHLSNIAEDQHHIRRSRAHQLA
                     GSAPREGSLAHALERAFAAGQGTPELAAFFDTARVTPVLTAHPTEVQRKSILNCQMVI
                     ARLLDERDRMQLTPEEEQANDEALRRAVLTLWQTRMLRTEKLSVMDEVSNGLSYFDYT
                     FLRELPRLYANLEDRLATRDRSWGALELPNFLQVGSWIGGDRDGNPFVTAEILEKALA
                     VQAEKVLTFYMDEVHTLGSQLSVAQGLVSASDALLRLAEASPDGSPHRIDEPYRRALT
                     GLYARLAATYAHLLGHTPPRHAVGPAAPYSHVDAFADDLEIVHRSLLANGLGALARGR
                     LRHLRRAVGVFGFHLAPIDLRQNSDVHERVVAELLRAITPDTDYLALDEAGRCALLLD
                     ELATPRPLASPWIDYSEESRGELAIFRAARAAHLRYGRAAVPNCIISKTDDVSDLLEV
                     AVLAKEAGLLRPAEGVLDVNIIPLFETIGDLRNAAGVMDRLFQLPGYRALLDSRHGEQ
                     EVMLGYSDSNKDGGFLTSGWELYKAEIGLVEVFARHGVRLRLFHGRGGSVGRGGGPSY
                     QAILAQPGGAVQGQIRLTEQGEVIASKYANPEVGRRNLEVLAAATLEATLFAPRDPAP
                     RPDYLEAMDELSADAFAAYRTLVYETDGFERYFWESTVIAEIAALNIGSRPASRKKST
                     AIEDLRAIPWVFSWAQCRLMLPGWYGFGSAVRAYLERHGDAGRARLQAMHKEWSFFAT
                     LLSNMDMVLAKSDIAIASRYAELVRDTALRDAIFPRIRQEHADTLEVLLAITGQQELL
                     DANPLLKRSMRNRFPYLDPLNHVQVELLHRYREGHQDERVRRGILLSINGIAAGLRNS
                     G"
     gene            10885..11814
                     /gene="hemC"
                     /locus_tag="PLH85_12465"
     CDS             10885..11814
                     /gene="hemC"
                     /locus_tag="PLH85_12465"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNB65426.1"
                     /translation="MSAPTPDRIVIATRESRLALWQAEHVKARLEALYPSCTVELLGM
                     TTRGDQILDRPLAKVGGKGLFVKELETALLDRRADIAVHSMKDVPMELPPEFALVAIS
                     EREVPLDAFVSNKYESLDDMPPGAVVGTSSLRREAQLHAQYPMLAVTSLRGNLDTRLR
                     KLDEGQYDAIILAAAGLTRLGLAARIRAVLPSTVSLPAAGQGALGIEALADRPEVAAW
                     MAPLAEAGTAACVRAERATSRALAGSCEVPLGAYAEVQDGQLWLRGFVALPDGSRIAR
                     AERRGPLAEPEALGLALAADLRADGADEILAGL"
     gene            11836..12609
                     /locus_tag="PLH85_12470"
     CDS             11836..12609
                     /locus_tag="PLH85_12470"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNB65427.1"
                     /translation="MSGLPLAGRTVAVTRPAAQADSLCAALVRLGAEPLRFPLLTVTA
                     VADPAPLQAVAGHLGDFDLAFFVSPNAVNFALDALLPVAPWPARLAVATVGLGSEAAL
                     AVRGFHDVIAPRAGFDSESVLALPEFQAEAVAGRRVLILRGDGGRDLLGNTLVERGAR
                     VDYLTCYHRGGPDADPAPLFEHARRGRLDALTVTSSEGVAHLLALPGWAVLADVPLFV
                     PHPRIAEAARRGGFATVIPTGPGDSGLLDGLRAHFAAAP"
     gene            12666..13760
                     /locus_tag="PLH85_12475"
     CDS             12666..13760
                     /locus_tag="PLH85_12475"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNB65428.1"
                     /translation="MSSELPSLPSVDAARPVDSTPPSPWRRPSAVIAAVAVALLGWQW
                     LETRSRLADLQDELARRLTDSDTAAKEGRALAKQAQEGVQDLQSKLGALEGRIAESQG
                     QQVALEAMYQEFSRTRDDRVLTEVEQAVTIAAQQLQLAGNVQAALFALQTADARLGSV
                     DRPHWLPLRKALAADMDSLKALPQIDAPGVALRLETLVGRIEGLPLAFEAHPRNEAAK
                     ADAAETSWIARLWSEFWNDFSQLVRIERLDRPDPALLAPSNVVFLRENLKLRLLNARL
                     ALMSRDGNTFREDVRVAAQWLERYFDGRNAAVATARTDLLELQKTPVGVDLPSLQASL
                     SAVQRLQAASERPVEAARPAAAAPARKSGR"
     gene            13763..14938
                     /locus_tag="PLH85_12480"
     CDS             13763..14938
                     /locus_tag="PLH85_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNB65429.1"
                     /translation="MRALLWVLGLFALAAGLAIAGRYNEGYALLVLPPYRVQISLNLL
                     LVLLLGGFVGIYALSRLVARTLALPGAVQRFRARKGRERAAQALYDAARLRIEGRFGQ
                     AMRQAAAAHEANESPGLAALLAARAAHSLRDDERYRAWLAKAAEHDDEVRVARLMTEA
                     ELAVEGRRFEEAAERIADLQAGGQRHIAALRLALRTAQARAQWSEVVRLVRQLVKVKS
                     LKPEQAELLKRQAHQEGLRQRHGDAAALNAYWQDVPRDEQRDARVVATLARALIAAGD
                     GVAAQQVIEKQLDMEWDSVLAGLYGRCEGGEVRSRLVRAEGWLKDHPRDPQLLLTLGR
                     LCVQSQLWGKAESYLDAALAIEPGWEAHVELAQLAERVGRTDDANRHYRAAAELSRR"
     gene            15380..15943
                     /gene="ahpC"
                     /locus_tag="PLH85_12485"
     CDS             15380..15943
                     /gene="ahpC"
                     /locus_tag="PLH85_12485"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNB65430.1"
                     /translation="MSIINTEIKPFKATAYANGKFVPVSDADLKGKWSVVFFYPADFT
                     FVCPTELGDLADVYPEFQKLGVEVYSVSTDTHFVHKAWADASDTIKKIKYVMIGDPTG
                     AITRNFDVMIEEEGLALRGTFVINPEGVIKVAEIHDLGIGRDAKELLRKVQAAQYVAS
                     HPGEVCPAKWTPGEATLSPSLDLVGKI"
     gene            16043..>17541
                     /gene="ahpF"
                     /locus_tag="PLH85_12490"
     CDS             16043..>17541
                     /gene="ahpF"
                     /locus_tag="PLH85_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNB65431.1"
                     /translation="MLDATLKTQLQAYLTKLTRPVDIRAALDDGETARQMAELLQEVA
                     ALSPLLNYVEAADAGVRTPSFQISRPGENTGIRFAAIPLGHEFTSLVLALLQVGGHPP
                     RVSDDTLAQIRALEGEFRFVTYISLSCQNCPEVVQSLNLMALINPRISHETVDGALFQ
                     DEVERLKIMGVPAIYLNGELFGQGRMELEEILAKVDSGAAARAAASIDARAPYDVLVV
                     GGGPAGASAAIYAARKGIRTGVVTERFGGQVLDTLAIENFISVKETEGPRLAAALEEH
                     VRQYEVDIVNLQRAEALVPGELIQVKLASGASLKARTVILSTGARWRDMNVPGETEYR
                     TKGVTYCPHCDGPLFKGKAVAVIGGGNSGVEAAIDLAGVVAHVTLLEFDSKLRADAVL
                     VRKLESLPNVRILVNAQTTQVNGDGEKVTGLTYKDRVSGEVHDVALAGIFVQIGLVPN
                     TAWLKGTIELSTRGEIIVDDHGRTSVPGVFAAGDVTTVPYKQIIIAMGDG"
BASE COUNT         2608 a         5931 c         6571 g         2431 t
ORIGIN      
        1 gatgcggcgg aagtgcgggc cctcggccgg cgcggcggcc tgggcgcgca ggatggccag
       61 gggctggagg ggatcgcggg agccggccag ggcgccgatg aacagcgccg cgccggtgag
      121 cagggcgacc acgccgatgc ccttccaaaa gcgctgccag ccggcggcat ggggcggcag
      181 cgggtcgagg gcgtgcaggt agatggccga catgatgagc agtccggccc aggccagcat
      241 gggcaccact gccggcagca ccggcgagac cagccacacc gcgacgccca gcagcatgac
      301 cccgaaggcc cgcttgacgc cttccatcca cgggccgggg cgcggcaggg cgctgcgggc
      361 ggctacaccg accagcagca ggggggcgcc catgcccagc gccatggtga acagggccgc
      421 accgccgagc agcgcgtcgc cgctccgggc gatgtagagg agcgcgccgg ccaggggcgc
      481 ggccacgcag gggccgacga tcagggccga cagggcgccc atcaggatga cgccggccgg
      541 cgagccgccg ggctggtgat tggcgcggtc ggacaggcgg ctctgcagcg cggtgggcag
      601 ttgcagctcg tagaagccga acatggacag ggacagggcc acgaagacca gggcgaagcc
      661 gcccagcacc cagggattct gcagggcggc cgacagcagg gtgccggaca gcccggcggc
      721 gacgccggcg gcggtgtagg tgagggccat ccccagcacg tagagcagcg acagggtgaa
      781 ggcccgcccg cgggagatgg cgtggccgtg gccgacgatg atgccggaga ggatcgggat
      841 catcgggaag acgcaggggg tgaaggcgag gagcaggccg aagccgaaga agctggcgag
      901 ggtggcgagg gtgccgccac cggccagcag gcgggcgatg cggccggatt cgtcgtgggc
      961 gtcggccggc acggcggtgg tggatgccgg tgcagtgagc ggggcctcgg cggccggtgc
     1021 ctggccgagg gcgcgggcga ggaagccacc gaccttgcgc tgtcccgcgg ggtcgatgct
     1081 caccgtctgg ggggtgggtg ggtagcagat gcccaggtcg gcgcagccct ggctggtgac
     1141 ggtgagcttg aagggcggct ggccggcgct gaggggcagg gggaagacca gttcgccccg
     1201 gtaggtttcg acttcgccgt agatgtcgtc cttcttgcgt ttgccgggcg gaagctgggc
     1261 ctcgccgaag gtgacgccgt cggcctcgaa cttgaacttg tgcttgtaga ggtaatagcc
     1321 ctcggcgacc tggaagcgga tctcgacggt gcggtcgtcg agggcgcggg cgtccaggtg
     1381 gaaggcctgc tcggcgggca gcggttcggc cgcggcggcc ggacgggcga cgaggaggcc
     1441 cagcatcagc agggcgggca gcagcagggc gaagaggcgg agcagggcgc ggttcatggc
     1501 gggcaagtgt agcgggtgcc ggcgacgggc gcgccgacgg agatcaagaa gccggagcgg
     1561 tttcgccggc cacccaggcg aggtaggcgg gcaggccctc gctgacaggg actgcgacga
     1621 tctccggaag ttcgtaggga tgggcggcgc gcagggtctc ctgcagggcg gcgtagcgtg
     1681 cgcggggggt cttgatgagg agcggaacct cggtggcggt ctcggtctgc ccctgccagc
     1741 gatagacgga ggtgcacggc gcctggatgc tcacgcaggc ggcgaggcgg gcgtccacca
     1801 gctggcgggc gatgcgtgca gcgctgtcgg cgtcgggcag ggtggtgaaa acgagcagga
     1861 tgtcggggtc ggccatgggc ggagtttact tcagcagacg ccgccgcatc aggacccggg
     1921 caacggcgaa ggcgatgccc gcgcaggcgg tcaggacgac caggtgcagc ccggcgtgga
     1981 cgggccagtg cccctgcagc agcgggcggg caagggccac ggcgtggctg agggggagca
     2041 gctcggcgag gagctggaag ccgccgggca gctggtcgcg gggaaagaac acgccggaca
     2101 ggagggtcat gggcgtgatg aagagggtga agtagtaggt gaagaagtcg tagcctggcg
     2161 ccagggcggt catgatcagg ccgagggcgg cgaaggcgag gccggtgagg aagaccaccg
     2221 gcagggcggc gagggccagg ggcgaggcgg tgtagccgaa cagggcgatc accgcgagga
     2281 tggcgaggcc ggccatggtg gctttgctgg cggcccacag gagttcggcg gcgacgatgt
     2341 catcgaggga cacgggggcg ttgaggatgg cgtcccaggt tttctgtacg tgcatgcgcg
     2401 agaagctgga gtacagcacc tcgaaggtgg ccgcgttcat cgtggaatag cacagggtgc
     2461 cgccggccag gaaggcgatg taggactggc cgccgacctc gggcaccagc atgcccaggc
     2521 cgtagccgag acccagcatg tagatcaccg gatcggccag gttgccgacg atggagggaa
     2581 gcgccagctt gcgccagacc aggaagttgc ggcgccacac ggcggcgatg cggccggaca
     2641 ggcgcggtgg ggcaggctgg ggggggttca tggggtctcc ggcgtgcggc cgtcgagcca
     2701 cgggaggtag tcccggtcgg gtccgagggc gtggtggtcc accaggtcgt aggcgaggaa
     2761 ctcgatgagg cgtgcggcgg gcagcacgcc gcgggcgctc tgggacgaga gggcgtcgag
     2821 cttggcggcg aggaggcggt gcgccttcat gtgctggtcc gtgcgggggt aggcgatctc
     2881 gccgaggagg gcctcctcgt cacggaagtg gcgctccgtg gtgtgggcca gttcgcggat
     2941 cgccacgtgg aggtcggcgg tggggccgtc gcgccggatc atctccagca gggcctgggc
     3001 gagggtgaag agttccttgt gctgatggtc catgagggga tggccaagcg tgtgcccgtc
     3061 ttcccagccg agccggtcga gacgagacag ctcggcgtgg gcgtggggct gatcatggcg
     3121 ctcgggcgcc gcgacgacca ggttgcggcc ctggcgcttg gcccggtaga gggccttgtc
     3181 ggtgcggtgc agccaggcgt gccagtcctc gtctgcctga cattcggcca cgccaaagct
     3241 ggcgctgagc ggaccggcgt ccgggaaggg ggccgcggcg atggcctggc gcagcttggc
     3301 cgccagggtc aggccgccga cgaggggggt gctgggcaac aggacgagga actcttcacc
     3361 gccgtagcgg ccgatcacgt ccaggtcgcg gcattcggcc aggatgcggc gggcgaattc
     3421 caccagcacc cgatcgccgg cctggtgacc gaggcggtcg ttgatgagct tgaagtggtc
     3481 gatgtccgcc atgatcaggg tgatcggatg accgaagcgg cggaagcgct gcatctcgct
     3541 ggcggcggct tcctcgaagc ggcgccgggt gagcaggccg gtcagggcgt cggtcgcgga
     3601 gcgggtctcg agttcggcca gggcctggcg caggatgtct tcgcgggttt cgtgatcggt
     3661 gaggtccacg aagcaggcga tgagcaggcc gtccgccacc tgcagctgga cacggatgtg
     3721 gagcgggccg gcggggccgc ggagcaggca ttcgacgctg tcgtggggcc ggatgtccgg
     3781 gtgcagcgcg gccaggtgcg ccggccagtc cggcggcggg ccgatgcggt cgccggcatc
     3841 ggcgtggcgg agccagtcgg tgagcggcat cccgagggtg gcgggggcgc tgaagagggc
     3901 cgcgaagccc cggttcgagc tcagaagccg accgtcagtg gcacgggcga ccgccagggc
     3961 gaccggcagg tgctcgatga tcgttcgggg gtcggggcac gcggacatgg ttgttcttgt
     4021 tggggcgtgg ataggcggga ctgtaccgcc tatcccccgt cctgtgggga aaaccccgct
     4081 catagccggg gtatgcggcg cacctcagtc gcgcagctcg cggccggtca gcttgaggaa
     4141 gacgtcctcg agattggcgg gccggtgcag ggtacgcagg ccgctctggt gggccagatg
     4201 ggcgagcagc ggggccgcgt cgtcggtgta acagaaggcc gtctcgccgc tcacctcgca
     4261 gcgccgggcg agggccgcgg catggcggga cgcccacgcc ggggcgccgc cgggggcatc
     4321 ggcccagtcg ccgaagactt ccaccacctg gggttcgatg tgggcggcga tgacctcccg
     4381 cgggctgccc tgggccagga tgcggccgcc gtcgaggatg gcgaggcggt cggcgaggcg
     4441 ctcggcctcg tccatgaagt gggtggtgag gagcaccgtg gtgccttcgg cgacgaggcg
     4501 gcgcaggcgt tcccagatga ggtggcgggc ttgcgggtcg aggccggtgg tgggctcgtc
     4561 gaggatcagc agttcgggac ggttgaccag ggcgcgggcc agggtcaggc gccgcttcat
     4621 gccgccggag agggacggga tgcgggcgtt ttccttgcct tccaggccgg cgaaggcgag
     4681 gagctcgggg atgcgcgggc ggatctcgct gtccgggatg ccgaagtagc cggcaaagac
     4741 gaggaggttc tcggcgcagg tgaaatccgg gtcgaggctg tcgagctggg ggaccacgcc
     4801 gacgcgggcg cgggcttcgc gcgcctgggc cggcaccggc aggccgcaca ggcggatgct
     4861 gccggcgctg gccgcggtga ggccgagggc gcagcgcagg gtggtggtct tgccggcgcc
     4921 gttggggccg agcagggcga agcattcgcc cgggcgcagg ctcaggtcga gcccgcgcac
     4981 cacctccctg gcgtcggcgc catggccgaa gcgcttgacg agaccggcga tctcgagggg
     5041 catcagacgg ccggcggcac gctggcgacg aaggacggcc gggccagcag gccggcggcc
     5101 caggcggcca gggcgggacg gccggcgcgc cagtccaggt gggcgaagcg caggtccaga
     5161 taacccaggg cgcagcccac ggcgatgtcg gcgagggtca gggtgtcgcc gccgaagccc
     5221 ttgcgggcgg cgaagcgttc ttccagtgcg cccaggccgc ggcggatctt ggtgatctgg
     5281 cgctccaggc tggccgggtc ctgctggggg gcggggcgac gcatctccag gaaggcgctc
     5341 accgccgcgt cggtgatgcc gtcggcgagg gcctcgctct ggcgtaccgg cagcgaggcg
     5401 atgcggtcgg cgggcagcag ggcggggccg ccgagggttt ccaggtattc ggcgatgacc
     5461 ggcgagtcga accacacctc gccgtcgtcc gccaccagca cgggcacctt gccgaggggg
     5521 ttgtagtcgg gcacgttggt ctcggccagc cacgggatgt ccacctgcag gtcgcagggc
     5581 aggccttttt cggcaaggat cacgcggatc ttgcgtgcga acgggctggt gagcgaaacg
     5641 atgagcttca tgggcggggt tcctgtcggg ggtcggggct cagaggggcg tccaggcccg
     5701 ttcgatgacg gttctgagga tgtgcaggcg gccatggaag aaatggtcgg cgcctggaat
     5761 gacgaccacc gggatttcct ggggttccgc ccattccatc acgttggcga ggggcacggt
     5821 ctcgtccttc tcgccatgga tgaccagggt gtcggccggg accggcagtg tggtgtagct
     5881 gcgggcgccc gtgacatagc cggccgcagt gcccaccagc acgatgcgct gggccggcgc
     5941 gacgctctcg gcgagccgcg cggcgacgcg ggtctgcacg taggcgccga aggagaagcc
     6001 gcccagggcg atgggcaggc tgccccagcg gctctcggcc cagctcagca cggccagcat
     6061 gtcgtcggtc tcggcatggc cgttgtcgtg ggtgccggcg ctcttgccca cgccgcggaa
     6121 gttggggcgg acgacggcgt agcccaggtc gcgcagcacc cgcgccaggg tgtgggccac
     6181 cttgttggtg ttggcgccgt ggaagagcgg atggggatgg cacaccaagg caatcccgcg
     6241 cacgtcggcg ggggtgtcga tgagcagctc gatggccccg gcgggaccgt tgatgagggc
     6301 ggattcggtg gcggcggggc ggttcatggc ggctcagatc ttgagacgtt cgacgatgcg
     6361 gccgtgctgc aggtgttcgt ggatgatctc gtcgatgtcg gccttgtcca cgaaggtgta
     6421 ccagacctcg tccgggtaga ccaccagcac cggcccttcg tcacagcggt cgaggcagcc
     6481 ggccttgttg atgcgcacct tgccacggcc cttgagaccg agggcggcga tgcggtcctt
     6541 ggcgtaggcc tgcagttcgg agccgccgcg ggcgttgcag caggtctcgc cttcggggcg
     6601 ctggttgcag cagatgaaca cgtggtgctt gaagtagctc atggggcggg attctccggt
     6661 aaggggcgcc tgcgattatc gtccaaacgc cgcccggcgg gcgcggctca gtgggggccg
     6721 gccccgtccc gtccgctgcg gccgcccagc aggcccagat agaggaaggc gaggaagggc
     6781 cacaggctcg cggcgaggga ggtcagccca tggaagttga ggaggttgcc ccgcagggcg
     6841 agcagggtgt cgttcggcag gaaggggttt tccggggcga gattgaccag ggcggtgccc
     6901 accaacaggg ccagcccggc caggaccgcg tgcaggcggg tgggcaggat caggcaggcg
     6961 gtgagcaggg cggcgcctgc ggcgatgccg atctgtccgc cgggcgtcgc ccagtggaag
     7021 gggtcgccgg gcgaaaagaa catgcccgcg gcgaggcttt ttgcccccag cccgaggagc
     7081 accagcagta ggatcgggaa ggggctcggg ctgcgcatca tgcagcgcgc ggtgaggccc
     7141 acggcgacca gctggctggc ggcgatggcg gcctccacct tgacgaagcg ctgggggttg
     7201 aaggggatgg gcgtgggcag gtcgaacagg cggcgcaggt cgccggtggc gaagagcagg
     7261 ctctccggcg tgagcagggt gagcagccac aggcccatca gcaccatgcc ggcctcgccc
     7321 acgtgcccgg ccacgatgcg gcgcgtgcgc cagcgcgcca gggcgccgcg ggtgtcaaac
     7381 aggggcgccc cccagcgggc gccggcgagg gctccgatga gccccccgag ggcgttgcag
     7441 cccagatcca cattggaggg gacgcgactg ggcaggaagt tctgcagcgt ctccaggccg
     7501 agactgagga gtccggcgat caccaccgcc atgcacaccg cgccgccggg ccgcaggcgc
     7561 ccgcccaggg ccgggacgag ggcgaagctg aagggcacga agcccagtac attcacgatc
     7621 aggtctgtct ggctgtaata gcgtggccac ggtgcggtca ggaagtcgag gagcggcagg
     7681 cccggatcgc gccagcccgt gaaggggtgc aggcaggcgt acacgatgag cacgccgatg
     7741 gccaccgcga aatgcagggc catgggggac ggacgtgaac gcaaggggat gggcatctcg
     7801 gcgcggcggg cgggcgacat ccggatttta ggccgggacg ggccgccctg tccgtttggc
     7861 gtgtgcggcg cagcaaatcg ccccggagcg gccgggcccg ccgctccggg gggccgcaac
     7921 cctcagccgc tgttgcgcag gccggcggcg atgccgttga tggagagcag gatgccgcgc
     7981 cgcacgcgct cgtcctggtg gccttcccga tagcggtgaa gcagctccac ctggacgtga
     8041 ttgaggggat ccaggtaggg gaagcggttg cgcatggagc gcttgaggag cgggttggcg
     8101 tcgagcagtt cctgctggcc ggtgatggcc aggagcacct ccagcgtgtc ggcatgttcc
     8161 tggcggatgc gcgggaagat cgcatcgcgc agggcggtgt cgcggaccag ctcggcgtag
     8221 cggctggcga tggcgatgtc gctcttggcc agcaccatgt ccatgttgga gagcagggtg
     8281 gcgaagaagg accattcctt gtgcatggcc tgcaggcgcg cccggccggc gtcgccgtgg
     8341 cgttccaggt aggcccgcac ggccgagccg aagccgtacc agccgggcag catgagacga
     8401 cactgggccc agctgaagac ccaggggatg gcgcgcaggt cctcgatggc ggtggacttc
     8461 ttgcgcgagg ccgggcggct gccgatgttg agggcggcga tctcggcgat caccgtggac
     8521 tcccagaagt agcgctcgaa gccgtcggtc tcgtagacca gcgtgcggta ggcggcgaag
     8581 gcgtcggcgg acagctcgtc catggcctcc agatagtcgg ggcgcggggc cgggtcgcgg
     8641 ggggcgaaga gggtggcctc cagggtggcg gcggcgagca cttccaggtt gcgccggccc
     8701 acttccgggt tggcgtactt ggaggcgatg acctcgccct gctcggtcag gcggatctgg
     8761 ccctgcacgg cgccgcccgg ctgggccagg atggcctggt agctggggcc gccgccgcgc
     8821 ccgacggagc cgccgcggcc gtggaacagg cgcaggcgca cgccgtggcg ggcgaagacc
     8881 tccaccaggc cgatctcggc cttgtagagc tcccagccgg aggtgaggaa gccgccgtcc
     8941 ttgttgctgt cggagtagcc cagcatcacc tcctgctcgc catggcggga atccagcagg
     9001 gcccggtagc caggcagctg gaacaggcgg tccatgacgc cggcggcgtt gcgcaggtcg
     9061 ccgatggtct cgaagagcgg gatgatgttc acgtccagca cgccctcggc cgggcgcagc
     9121 aggccggcct ccttggcgag cacggccacc tccagcaggt cggagacgtc gtcggtcttg
     9181 gagatgatgc agttgggcac cgccgcccgg ccatagcgca ggtgggcggc gcgggcggcg
     9241 cggaagatcg ccagctcgcc gcgggattcc tcggaatagt cgatccacgg cgaggcgagg
     9301 ggccgcgggg tggccagttc gtccagcagc agggcgcagc ggccggcctc gtcgagggcc
     9361 aggtagtcgg tgtccggggt gatggcgcgc agcagctcag ccaccacacg ctcgtgcacg
     9421 tcggagttct ggcgcaggtc gatgggcgcc aggtggaagc cgaacacccc caccgcccgg
     9481 cgcaggtggc gcaggcggcc gcgggccagg gcgcccaggc cgttggccag gagggagcga
     9541 tgcacgatct ccaggtcgtc ggcaaaggcg tccacatggg aatagggcgc tgcggggccc
     9601 accgcatggc gcggcggggt atggccgagc aggtgcgcgt aggtggcggc gaggcgcgcg
     9661 tagaggccgg tgagggcgcg ccggtagggc tcgtcgatgc ggtggggcga gccgtccggc
     9721 gaggcttcgg cgaggcgcag cagggcgtcc gaggcgctca ccagcccttg ggcgacggag
     9781 agctgggagc ccagggtgtg cacctcgtcc atgtagaagg tgaggacctt ctcggcctgc
     9841 acggcgaggg ccttttcgag gatctcggcg gtgacgaagg ggttgccgtc ccggtcgccg
     9901 ccgatccagc tgcccacctg caggaagttg ggcagctcca gtgcgcccca gctgcgatcg
     9961 cgggtggcga ggcggtcttc caggttggcg tagaggcgcg gcagctcccg caggaaggtg
    10021 tagtcgaagt aggacaggcc gttgctcacc tcgtccatca ccgacagctt ctcggtacgc
    10081 agcatgcgcg tctgccacag ggtcagcacg gcgcggcgca gggcctcgtc gttggcctgc
    10141 tcctcttccg gggtgagctg catgcggtcc cgttcatcga gcaggcgcgc gatcaccatc
    10201 tggcagttga ggatgctctt gcgctgcact tcggtggggt gggcggtgag caccggggtg
    10261 acccgcgccg tgtcgaagaa ggccgccagc tcgggcgtgc cctggccggc ggcgaaggcg
    10321 cgctccaggg cgtgggcgag gctgccttcc cgcggcgccg agccggccag ctggtgggcg
    10381 cgggagcggc ggatgtggtg ctggtcctcg gcgatgttgg agaggtgcga gaagtagctg
    10441 aacgcacgca ccacgtcgat ggtctggtcg cgggacaggg aatccagcgt ggcttccagc
    10501 tccttgcggg ccgccgggtc ggcgtggcgg cggaagcgga tggagctctg gcggatcgtc
    10561 tccaccaggt cgaacaccgc ttcgccctcc tgggcgcgca gggtgtcgcc cagcacgcgg
    10621 ccaagcagac ggatatcgtc acggaggggg aggtccttgt cttcgctcat ggggagtcct
    10681 ggaaggttcg agggggccgc cggcagcggg ccgcggggtc aagggctcca tgctagaatc
    10741 cgcgacccga tggacgccat tggtggcaac cctgatgctg cgctgcatca gcgcgcaatc
    10801 tgcgccctcc gcccggtctt gtccatcccc cgaacgccaa ccggagcccc gcccgggctc
    10861 cccacgaccg ccgagccgac taccgtgagc gcccctacgc ccgaccgcat cgtcatcgcc
    10921 acccgtgaaa gccgcctcgc cctgtggcag gcggagcacg tcaaggcccg ccttgaggcc
    10981 ctctacccgt cctgcacggt cgaactgctg ggcatgacca cccgcggcga ccagatcctc
    11041 gaccggcccc tcgccaaggt cggcggcaag ggcctgttcg tcaaggaact ggaaactgcc
    11101 ctgctggacc gccgcgccga catcgccgtg cattccatga aggacgtgcc catggagctg
    11161 ccgccggagt tcgccctggt ggccatctcc gaacgggagg tgccgctgga cgcattcgtc
    11221 tccaacaagt acgaaagcct cgacgacatg ccgccgggcg ccgtggtggg cacctccagc
    11281 ctgcgccgcg aggcccagct ccacgcccag tatccgatgc tcgccgtcac cagcctgcgc
    11341 ggcaacctgg acacccgcct gcgcaagctg gacgaaggcc agtacgacgc catcatcctc
    11401 gccgccgccg gcctcacgcg cctcggcctg gccgcgcgca tccgcgccgt gctgccctcc
    11461 accgtctccc tgccggccgc cggccagggc gccctgggca tcgaggccct ggccgaccgc
    11521 cccgaagtcg ccgcctggat ggcgcccctc gccgaagccg gcaccgccgc ctgcgtgcgc
    11581 gccgagcggg ccacctcccg tgccctggcg ggcagctgcg aggtgcccct gggcgcctac
    11641 gccgaggtgc aggacggcca gctctggctg cgcggcttcg tcgccctgcc cgatggctcc
    11701 cgcatcgccc gcgccgagcg tcgtggtccc ctcgccgaac cggaagccct cggcctggcc
    11761 ctggccgccg acctgcgcgc cgacggcgcc gacgagatcc tcgccggcct ctgagcgccg
    11821 cccgcggagc gtcccatgag cggcctgccc ctcgccggac gcaccgtcgc cgtcacccgc
    11881 ccggccgccc aggccgacag cctgtgtgcg gcgctggtcc ggctgggcgc cgagcccctg
    11941 cgctttccgc tgctcaccgt cacggcggtg gccgatccgg ccccgctgca ggccgtcgcc
    12001 gggcatctgg gcgacttcga tctggccttc ttcgtcagtc ccaacgcggt caatttcgcc
    12061 ctcgacgcgc tgctgccggt ggccccctgg ccggcccggc tcgccgtcgc cacggtcgga
    12121 ctgggcagcg aggcggctct ggcggtgcgc ggcttccatg acgtgatcgc gccgcgcgcc
    12181 ggcttcgaca gcgagtcggt gctggccctg ccggaattcc aggccgaggc tgtggccggc
    12241 cggcgcgtgc tgatcctgcg cggcgacggc gggcgcgacc tgctcggcaa caccctcgtc
    12301 gagcgcggcg cccgcgtgga ctacctcacc tgctaccacc ggggcggccc ggacgccgac
    12361 ccggcgcccc tcttcgagca cgcccgcagg ggccgcctcg acgccctcac ggtgaccagc
    12421 agcgaaggcg tcgcccatct gctcgccctg ccgggctggg ccgtgctggc agacgtgccc
    12481 ctcttcgtgc cccatccgcg catcgccgag gccgcccggc ggggtggatt tgccacggtg
    12541 atccccaccg gccccggcga cagcggtctc ctcgacggcc tgcgcgccca ctttgccgcc
    12601 gcgccctgaa gcgtgtcgcg actgctcccc cttcgtcgac gtcctatccg ggttaaactc
    12661 acgccatgag cagcgaactc cccagcctcc cgtccgtgga cgccgcccgt cccgtcgatt
    12721 ccacgccgcc cagtccgtgg cggcgcccgt ccgccgtgat cgccgccgtg gccgtcgccc
    12781 tgctgggctg gcaatggctc gagacccgca gccgtctcgc cgacctgcag gacgaactgg
    12841 cgcggcgcct caccgacagc gacaccgccg ccaaggaagg ccgcgcgctg gccaagcagg
    12901 cccaggaagg cgtgcaggat cttcagtcca agcttggcgc cctcgaaggc cgcatcgccg
    12961 aatcccaggg tcagcaggtg gcgctggagg ccatgtacca ggaattctcc cgcacccgcg
    13021 acgaccgggt gctgaccgaa gtggagcagg ccgtcaccat cgccgcccag cagcttcagc
    13081 tggccggcaa cgtgcaggct gccctgttcg ccctgcagac cgccgacgcg cgcctgggca
    13141 gcgtcgatcg tccccactgg ctgcccctgc gcaaggccct ggcggccgac atggacagcc
    13201 tcaaggccct gccccagatc gacgcaccgg gcgtcgccct gcgcctggag accctggtcg
    13261 gccgcatcga aggcctgccc ctcgcctttg aggcccatcc ccgcaacgaa gccgccaagg
    13321 ccgacgcggc cgagaccagc tggatcgcgc gcctgtggtc ggagttctgg aacgacttca
    13381 gccagctcgt gcgcatcgag cgtctcgacc gccccgatcc ggccctcctc gcgccgagca
    13441 acgtggtctt cctgcgggag aacctcaagc tgcgcctgct caatgcccgc ctcgccctga
    13501 tgtcgcggga cggcaacacc ttccgcgaag acgtgcgggt ggccgcccag tggctggagc
    13561 gctatttcga cgggcgcaac gccgccgtcg ccaccgcccg cacggatctg ctcgagctgc
    13621 agaagacgcc ggtgggggtg gacctgccca gcctgcaggc cagcctcagc gcggtgcagc
    13681 gcctgcaggc cgcctccgaa cgccccgtcg aggcggccag gccggctgcc gcggcgccgg
    13741 cccgcaagtc ggggcgctga tcatgcgggc cttgctgtgg gtgctgggcc tctttgcgct
    13801 ggccgccggg ctggccatcg ccggtcgtta caacgaaggc tacgcgctgc tcgtgctgcc
    13861 gccctaccgg gtgcagatct ccctcaacct cctgctcgtg ctgctgctgg gcggtttcgt
    13921 ggggatttac gccctctcgc ggctggtggc ccggaccctc gccctgcccg gcgcggtgca
    13981 gcgcttccgt gcccgcaagg ggcgtgagcg cgccgcccag gccctctacg atgccgcccg
    14041 tctgcgcatc gaagggcgct tcggccaggc catgcgccag gccgccgccg cccatgaagc
    14101 gaacgaatcc cccggactcg ccgcactcct cgctgcccgc gccgcccatt ccctgcggga
    14161 cgacgagcgc taccgggcgt ggctcgccaa ggctgccgag cacgacgacg aagtgcgcgt
    14221 cgcccgcctc atgaccgaag ccgagctggc tgtcgaaggc cgccgcttcg aggaggccgc
    14281 cgagcgcatc gccgacctcc aggccggcgg ccagcgtcac atcgccgccc tgcgtctggc
    14341 cctgcgcacc gcccaggccc gcgcccagtg gagcgaggtg gtgcggctgg tgcgccagct
    14401 cgtcaaggtc aagtccctca aacccgagca ggccgagctc ctcaagcgcc aggcccacca
    14461 ggaagggctg cgccagcgcc acggcgacgc cgccgcgctc aacgcctact ggcaggacgt
    14521 gccgcgggac gagcagcgcg acgcccgcgt ggtcgccacc ctggcccgcg ccctgatcgc
    14581 ggccggcgac ggcgtggcgg cccagcaggt catcgagaag cagctcgaca tggagtggga
    14641 ttccgtgctc gcaggcctct acggccgctg cgagggcggc gaggtgcgca gccggctggt
    14701 gcgcgccgag ggctggctca aggatcatcc ccgcgacccg cagctgctcc tcaccctggg
    14761 ccgcctgtgc gtccagtccc agctttgggg caaggccgaa agctacctgg acgccgcgct
    14821 ggccatcgag ccgggctggg aagcccacgt ggagctggcc cagctggccg agcgcgtcgg
    14881 ccgcaccgac gacgccaacc gccactaccg cgccgcggcc gagctgtccc ggcgctgagc
    14941 gccccgcggc ggaacgatcc gcccagatcc cgcatctcag cccgatgcct gcggggcccc
    15001 tgtccggcag gcatcgcccg gcgccacagc cggcgcgcca ggcgtcccgt cgtccgaccc
    15061 cgtcgaggat agaccccgcc ccgccgcccg gcccctgcgc cgtgtggcct cactgtccgc
    15121 ggctgacgcc cggctcaccg tctgcgttcc tgccgggggc gcaccatggg ccgcgttccg
    15181 gcccgcatcg agcacgtcgg ccgccgcaca ggccccttgt cctgggcgag cgcgtcccgc
    15241 cagcacggtc atatttcagc cataatggcg aaaccgatag ctttctttaa tcgggatcat
    15301 tttcagagtc aatttgagta cgcggcgcag ccctcttatc atggcgtctc cgcatcccac
    15361 tccacatgaa ggaaagccca tgtccatcat caacaccgaa atcaaaccct tcaaggccac
    15421 cgcctacgcc aacggcaagt tcgtcccggt ttccgatgcc gacctgaagg gcaagtggtc
    15481 cgtggtgttc ttctaccccg ccgacttcac cttcgtctgc cccaccgagc tgggcgacct
    15541 ggctgacgtc taccccgaat tccagaagct gggcgtcgag gtgtactccg tctccaccga
    15601 cacccacttc gtgcacaagg cctgggccga cgcctccgac accatcaaga agatcaagta
    15661 cgtgatgatc ggcgacccca ccggcgccat cacccgcaac ttcgacgtga tgatcgaaga
    15721 agaaggcctg gccctgcgcg gcaccttcgt gatcaacccc gaaggcgtca tcaaggtcgc
    15781 cgaaatccac gacctgggca tcggccgtga cgccaaggaa ctgctgcgca aggtgcaggc
    15841 cgcccagtac gtggcctccc acccgggcga agtctgcccc gccaagtgga ccccgggcga
    15901 agccaccctg tccccgtccc tggacctggt cggcaagatc taagccgcct gcccggacac
    15961 ctgtcgtacc gcaacgcccg ggcagcctgc cggcgcccgg gcgttgcttt atccggattc
    16021 tgactttttc aaggagccca tcatgctgga cgccaccctc aagacccaac tccaggccta
    16081 tctcaccaag ctcacccgcc cggtggacat ccgcgccgcc ctggacgacg gcgagaccgc
    16141 gcggcagatg gccgagctcc tgcaggaagt ggcggccctg tcgcccctgc tgaattacgt
    16201 ggaagccgcc gatgccggcg tgcgcacccc cagcttccag atcagccgcc ccggcgaaaa
    16261 caccggcatc cgctttgccg ccatccccct gggccacgaa ttcacctccc tggtgctggc
    16321 cctgctccag gtgggcggcc atccgccccg cgtcagcgac gacaccctcg cccagatccg
    16381 cgccctcgaa ggcgagttcc gcttcgtcac ctacatctcc ctgtcctgcc agaactgccc
    16441 cgaggtggtc cagtccctca acctgatggc cctcatcaat ccgcgcatct cccacgagac
    16501 cgtggacggc gccctgttcc aggacgaagt cgagcgcctc aagatcatgg gcgtgcccgc
    16561 catctacctc aacggcgagc tcttcggcca gggccgcatg gagctggaag agatcctcgc
    16621 caaggtggat tccggcgccg ccgcccgcgc cgcggccagc atcgacgccc gcgcgcccta
    16681 cgacgtgctg gtggtgggcg gcggcccggc cggcgcctcg gccgccatct acgccgcgcg
    16741 caagggcatc cgcaccggcg tggtcaccga gcgcttcggc ggccaggtgc tggacaccct
    16801 cgccatcgag aacttcatct ccgtcaagga gaccgaaggc ccgcgcctcg ccgccgcgct
    16861 ggaagagcac gtgcgccagt acgaggtgga catcgtcaac ctgcagcgcg ccgaagccct
    16921 ggtgccgggc gagctgatcc aggtgaagct ggccagcggc gccagcctca aggccaggac
    16981 ggtgatcctg tccaccggcg cccgctggcg cgacatgaac gtgcccggcg agaccgagta
    17041 ccgcaccaag ggcgtcacct actgccccca ctgcgacggc cccctcttca agggcaaggc
    17101 cgtggcggtg atcggcggcg gtaactccgg cgtcgaggcc gccatcgacc tggccggcgt
    17161 ggtcgcccac gtcaccctgc tggagttcga cagcaaactg cgcgccgacg cggtgctggt
    17221 ccgcaagctc gaaagcctgc ccaatgtgcg catcctggta aacgcccaga ccacccaggt
    17281 gaatggcgac ggcgaaaagg tcaccggcct cacctacaag gaccgggtca gcggcgaggt
    17341 gcatgacgtc gccctcgccg gcatcttcgt gcagatcggc ctggtgccca acaccgcctg
    17401 gctcaagggc accatcgaac tgtcgacccg cggcgagatc atcgtggacg accacggccg
    17461 cacctccgtg cccggcgtct tcgccgccgg cgacgtgacc accgtgccct acaagcagat
    17521 catcatcgcc atgggcgacg g
//
LOCUS       DAOQMM010000080        17060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1278_length_17060_cov_50.500617, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000080 DAOQMM010000000
VERSION     DAOQMM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17060
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1278_length_17060_cov_50.500617"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PLH85_12495"
     CDS             <1..173
                     /locus_tag="PLH85_12495"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNB65432.1"
                     /translation="TERAYDNPKFVEDMVRDVAGCLNREGRIDAYVVESENFESIHNH
                     SAYALIEHDKRL"
     gene            complement(267..674)
                     /gene="rplS"
                     /locus_tag="PLH85_12500"
     CDS             complement(267..674)
                     /gene="rplS"
                     /locus_tag="PLH85_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNB65433.1"
                     /translation="MSNIIQQLEQEEIARLTQGKTIPEFAPGDTVVVQVKVKEGNRER
                     LQAYEGVVIAKRNRGLNSAFIVRKISSGEGVERTFQTYSPLVASIEVKRRGDVRRAKL
                     YYLRQRSGKSARIKEKLDHKSFAAKAAAAKAQA"
     gene            complement(762..1544)
                     /gene="trmD"
                     /locus_tag="PLH85_12505"
     CDS             complement(762..1544)
                     /gene="trmD"
                     /locus_tag="PLH85_12505"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNB65434.1"
                     /translation="MVTAGAGKAYDVVTLFPEMFAALTGCGITGRAQERGLYGLTCWN
                     PRDFAEDRYRTVDDRPYGGGPGMVMLPGPLEKAIAAAKARQVGLTGQAGRVIYLSPQG
                     RPLDHARVLELVAEPALVLLCGRYEGVDQRLIERCVDEEVSLGDFVLSGGELPAMVLL
                     DAMIRQLPGALNDEDSAVEDSFVDGLLDCPHYTRPVMYEGLAVPDVLLSGNHAEIRRW
                     RLQQSLGRTEVRRPDLLARRGVLSKEESRLLEAFRQARRKPA"
     gene            complement(1538..2041)
                     /gene="rimM"
                     /locus_tag="PLH85_12510"
     CDS             complement(1538..2041)
                     /gene="rimM"
                     /locus_tag="PLH85_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300530.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNB65435.1"
                     /translation="MIVLGRIVAPYGVAGWVRVRPFGDDPLSWKKMPQWFLGTDPDGQ
                     DWAPYTLKGCRAHGKGDLVATFAEVPDRNAAEAVDGFYVAVPREALPKPDKDEYYWAD
                     LIGLEVWNTQDVRLGTVSGLLSTGAHDVLQVQDGEVERLIPFVASYVTDVDVAARRIR
                     VEWEADW"
     gene            complement(2078..2326)
                     /gene="rpsP"
                     /locus_tag="PLH85_12515"
     CDS             complement(2078..2326)
                     /gene="rpsP"
                     /locus_tag="PLH85_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNB65436.1"
                     /translation="MVVIRLARSGSKKRPFYNIVAADSRNRRDGRFIERIGYYNPVAS
                     GNEKGLVVNVERLSHWTGNGAQLSDTVARLVKQAAKAA"
     gene            complement(2485..3792)
                     /locus_tag="PLH85_12520"
     CDS             complement(2485..3792)
                     /locus_tag="PLH85_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNB65437.1"
                     /translation="MHKTLIAALLGALAAGTALATTEADVENSFNPYKGGFPKAAGLT
                     AGTVINKGNAEQFKDAIPAGVLRLLKEGLLEIKVGPTTQFELSKAYVEATRKNLGKAK
                     LGPKNGDLEGYVAGRPFPEEPDAKDPRAGEKLAWNYKYGVNWGDGAAIYPFYWKYRNM
                     QSGQVEKTIKFNFHFLNFKHRVKDAPVPEVTPNPSELFRAIYVKALDPQDLKNTQLLI
                     QRYEDDQKLDDAYLYLGFQRRVRRLATGQTTDAFLGSDLMIEDFEGYNGRISDMKWTY
                     KGTKNVILPMWNHNELKLAGDMPAEADGYKYVDFGGQGGCFHNGTWQLRKVYVLEAAP
                     VNPNHPISKRVFYMDAQLGNLNGANEIYDRKGELWKVFMVGKSHPDAHLPINKGAGIG
                     IDDAFSMVDVQSKHCTTGQFKGQVDPKLNPPSMFQVQYMRGGD"
     gene            complement(3806..5776)
                     /locus_tag="PLH85_12525"
     CDS             complement(3806..5776)
                     /locus_tag="PLH85_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HNB65438.1"
                     /translation="MKNRSSRFQPALSLCALAVSAAFAVPVLADELPDLGGGGAEPWR
                     VDTYYENHTAGRGKDNTGRSVGLSKFRNTLQVEADKNAGNGWNIHSILRGTWDGVYRL
                     NSSEYGKDAGGPILLENTGGATVGMPTMVPQGGGLGHTAVAGAFGLTNNAFVDSYPGN
                     PNDGLRVLGDRWHAQAGGVAFGVPVRPCDYDNRGCVNFGGYGNKTLSELELPEFNSRL
                     DFIRELYARNSFTLSDGTELFVKVGKQQVVWGRTDLFRVLDVINPVDYSRNNIYDELQ
                     DIRIPMWILQTEWRMGASESMQDRNLQVVWNFDQFRANNLGQCGTPNVILDAGCFFRG
                     MKGLWDTGGTVANFAGGTLATNFGPGQIGIRNVNLPAWSFNNTQLGVKFEGITKDGLS
                     FSLNGLTYRSQLPSLRAFNSAQNPFTGVNPDPTSHLIAFDMYFPRVNLIGGSLDLQSE
                     TLGAAFRLEGALTTGEEFANTARPELYSKNRVWRSVIGMDRPTFVPWINPHRTTLFSA
                     QLFYQHIFDHELRQGAYGPVGMPDWEDNVIGTLLMKGFMHGDRVSPQVILARDFRARA
                     QTVAPSLDWLVSNDLKLTFGANLKGGSNSRNQFDDCRSCNPYNPFTAYPGQTGAGSYG
                     LGGYEPLGRFRAGPIGTAMAENEIFLMARYKF"
     gene            complement(5867..8257)
                     /locus_tag="PLH85_12530"
     CDS             complement(5867..8257)
                     /locus_tag="PLH85_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNB65439.1"
                     /translation="MVSQFRHRITHTLHRAEEWLFANPKIVLAAILIVTALFGVALPK
                     LRIYSDFADLLPQNHPYIKVYNRLKENFGGANMIVMAIEVEKGTIFNDETLALIHKAT
                     QGIDSLPGVNHNLVGSLTHRTARKIFLDESGGFASESYYDPMKSGRTVAELEQLKKDV
                     IANPTIYGLLVSPDLKAALIKAQLVEGDIDYPKTFAALQEVRQSLATPGHALYVTGNP
                     VLTGWVYTYLNQIVEILAWTLALLTFLLVIYFRRLYGIALPLLGIALSTIWGLGFMAL
                     MGINLEPLSLPIPFLIAARATSHGVQLVVRYYEELAIVKDGRKAARNALDALFRPGSL
                     AIIVDAVGIAVLVLGAAPFNHKLGLAAGFWGFSVIFTVHFMVPLALTVLPAPKNTVNA
                     NEAVRNFLGAAMARTGGTDGGARSILLLTLIGAVGGLYFVGQVQLGEAEPGSPLLHTH
                     HDYNVSTRAINTRFPGSEELHVLMRTDEKGGIKRPEVMAAIERFQAHMLSDPTLGGAK
                     ALPGVIRVVNKLTHNDDPRWMQVPDTADEVGGLMFAYMAASPIPGALKEFVNADENEA
                     DMVFYYKDHQAETIRRVVALAEAGKRQLEAEVKGIHVELGGGIVGVTAAGNQALHTDH
                     LIIIPTVMLIAFVLVMVYYSSLHAGWLMVLPMLFATLMTYAYMGAAHIGINVNTVPVI
                     AVGVGVGIDYAVYFMDRIREEYAALHDIKQAVIRAVATTGYAVSFTAATLIAGVVMWI
                     FMSDLRFQSDAAVLLSFMLIVNAIAAVLIVPSWCVVFKPKFVTAIHYDEDGVLEAD"
     gene            complement(8267..9253)
                     /locus_tag="PLH85_12535"
     CDS             complement(8267..9253)
                     /locus_tag="PLH85_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNB65440.1"
                     /translation="MKHAGKRCARTALNVALMACLALAGLTGCQSAADLSAVNAEAAR
                     PVQRYDITQALAANGRTVVAGTQSGAVLVSADQGRSWQRQMLAGASLVGLAACPDGSF
                     VGVDFFHKVWRADAAGTQWSAVPLDKPRTPLAVSCDAQGRWWVAGTAATLAVSADRGA
                     TWQVSELAEDAQLTGVQMVDARFGIATGEFGLIAVTTDGGATWSRRGKMPDEFYPYAS
                     LFVSPAEGWVSGLAGQILHTTDGGLSWTRQPNASQMPLYRLFLHEGIPYGVGAGGTVA
                     RLDGGQWQPVAYPDPLPVFLGAAASLPEQHALVIGGPGGLLRVIGTQALAAR"
     gene            complement(9375..9569)
                     /locus_tag="PLH85_12540"
                     /pseudo
     CDS             complement(9375..9569)
                     /locus_tag="PLH85_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
     gene            complement(9592..10380)
                     /gene="dmpH"
                     /locus_tag="PLH85_12545"
     CDS             complement(9592..10380)
                     /gene="dmpH"
                     /locus_tag="PLH85_12545"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HNB65441.1"
                     /translation="MKLDQATIARLAEHLEDCELQARDTLKITDEFPDMDWEDAYAIQ
                     DAIRARKRARGTRITGLKAGLTSYAKMKQMGVETPCFGFITDYGAIPDGGEVKVSELI
                     HPKVEPEIAFVMKQALKGPGCHIGAVLAATDFVIPGMEVIDSRYRDFKFDLKSVIADN
                     TSAARYVVGGQPLPVSEVDLRTVGIVMEKNGEPVAFGAGAAVLGHPAAAIAMLANHLG
                     ARGEEIPAGALILSGGITEAVAVQAGDHVTLRVQGMGSVSLRFK"
     gene            complement(10377..11408)
                     /gene="dmpG"
                     /locus_tag="PLH85_12550"
     CDS             complement(10377..11408)
                     /gene="dmpG"
                     /locus_tag="PLH85_12550"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HNB65442.1"
                     /translation="MNLQGKKVTVHDMTLRDGMHPKRHQMTLDQMVAIATGLDAAGVP
                     LIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPKMKQAKVSALLLPGIGTVEHLKMAR
                     DLGVHTIRVATHCTEADVSEQHITLARKLEMDTVGFLMMSHMNSPEGLVKQARLMEGY
                     GANCIYITDSAGHLLPDGVKARLGAVREALKPETELGFHGHHNLAMGVANSIAAIEVG
                     ATRIDAAAAGLGAGAGNTPMEVFCAVADLMGIATGVDVAKITDVAEDLVFPIMDFPIR
                     IDRDALTLGYAGVYGSFLLFAKRAAVKYGVPAREILVELGRRGMVGGQEDMIEDTAIT
                     MAKARGLTV"
     gene            complement(11427..12353)
                     /locus_tag="PLH85_12555"
     CDS             complement(11427..12353)
                     /locus_tag="PLH85_12555"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HNB65443.1"
                     /translation="MSKKIKCALIGPGNIGTDLLAKLQRSPVLEPVWMVGIDPDSDGL
                     KRAREMGLKTTADGVDGLLPHVLADGVQIAFDATSAYVHAENSRKLNALGVLMIDLTP
                     AAIGPYCVPPVNLLAHVGKGEMNVNMVTCGGQATIPMVAAVSRVQKVSYGEIVATVSS
                     KSAGPGTRKNIDEFTRTTAGAVEKVGGAEKGKAIIIINPAEPPLMMRDTVHCLVEGEP
                     KRDEITASIHAMIREVQKYVPGYRLVNGPVFDGNRVSVYLEVEGLGDYLPKYAGNLDI
                     MTAAGARTAEMFAEEILAGRLVLEPKAAASAH"
     gene            complement(12383..13177)
                     /locus_tag="PLH85_12560"
     CDS             complement(12383..13177)
                     /locus_tag="PLH85_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB65444.1"
                     /translation="MDLHLNGKRALVTGSTAGIGFATARALAQEGAEVIVNGRSRAGV
                     ADAVARLQAVVPDAKVSGVAADLSSATGCEALTAACPDVDVLVNNLGIFDPKPFESIP
                     DLEWFHFFETNVMSGVRLCRHYLPAMKARDWGRIVFISSESGVCPPAEMVHYGMSKAA
                     QISVARGLAETCTGSGVTVNSVLPGPTRTEGVATFFGRLADEQGVSVEQAERDFFAHA
                     RPTSLLKRFIEPAEVAALVAYVCSPLASATHGAALRVEGGVVRSMV"
     gene            complement(13188..13970)
                     /gene="dmpE"
                     /locus_tag="PLH85_12565"
     CDS             complement(13188..13970)
                     /gene="dmpE"
                     /locus_tag="PLH85_12565"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HNB65445.1"
                     /translation="MDKALITQLGDELYDALIQRRTLAPLTERHPDITIEDAYQIQQQ
                     MIARRLAAGERPVGKKIGVTSKAVMNMLGVHQPDFGWLLDGMVYNEGQAIPAATLIQP
                     RAEGEIAFLMKKDVMGPGLSGADILAATEGVMACFEIVDSRIHDWKIKIQDTVADNAS
                     CGVLVLGDRIVDPRKVDLATCGMVLEKNGDIVGTGAGAAALGHPLNAMAWLANTLGRL
                     GIGLKAGDIVLSGSLAALIPVKAGDSLRVTIGGIGGCSVRFS"
     gene            complement(13980..14834)
                     /locus_tag="PLH85_12570"
     CDS             complement(13980..14834)
                     /locus_tag="PLH85_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB65446.1"
                     /translation="MSTASPEIGRTLRAAGRQTNVHDQGSGAPVTLIHGSGPGVSAWA
                     NWRLVMPALAERRRVVAPDMAGFGFTERFDGESYDMQGWVGHALGVLDALEIEQTDLV
                     GNSFGGAIALALAIQHPERVRRLVLMGSVGVPFEITPALDAVWGYAPSIEAMRRLLDL
                     FAYDRGLVNDELARLRYEASVRPGFQESFGAMFPAPRQRWVDAMTSAEADIRALPNQT
                     LVIHGREDQVIPLANSLTLATWIPRAQLHVFGQCGHWTQIEHAARFARLVGDFLAEAA
                     DDEPQPLS"
     gene            complement(14849..16309)
                     /locus_tag="PLH85_12575"
     CDS             complement(14849..16309)
                     /locus_tag="PLH85_12575"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HNB65447.1"
                     /translation="MNRIKHFINGEFVEAASGRLFDKHSPLDNRVIAQVSEAGRPEVD
                     AAVGAARAALEGPWGQITVAERVDLLYGVANEINRRFEDFLSAEVADTGKPRSLARHL
                     DIPRGAANFKIFADVVKNVPTEFFEMATPDGRGALNYAIRRPVGVVAVVCPWNLPLLL
                     MTWKVGPALACGNAVVVKPSEETPQTATLLGEVMNAVGIPRGVYNVVHGFGPGSAGEF
                     LTTHPGVNAITFTGETRTGAAIMRAAADGARPVSLEMGGKNPAIVFADADLDAAIEGT
                     LRSCFANCGQVCLGTERVYVERPLFDTFVARLKQGAESLVIGHPDSAETGMGPLISQE
                     HKQKVLGYYRKAVEEGATVVTGGGVPEVPAELKDGAWVQPTIWTGLGDDAAIAREEIF
                     GPCTLVMPFDSEDEVVRRANATTYGLAASVWTRDVTRAHRVAGKLETGLVWVNSWFLR
                     DLRTPFGGAKQSGIGREGGVHSLEFYTELKNICIKL"
     gene            complement(16342..16785)
                     /locus_tag="PLH85_12580"
     CDS             complement(16342..16785)
                     /locus_tag="PLH85_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNB65448.1"
                     /translation="MNATRPEPVAVTQSVISAEASVVALQAAVSRAMELGVRINVAVA
                     DSGGNLAGFLRMPGAFLQSIDIAIDKAYTAAGFGFSTKDWMKLIGHDEGMKLGFSARP
                     RLVVFGGGLPIRVGGELIGGIGVSGASEAQDEDCARAALAALGAE"
BASE COUNT         2697 a         5371 c         6047 g         2945 t
ORIGIN      
        1 tcaccgagcg cgcctacgac aaccccaagt tcgtcgagga catggtgcgc gacgtggccg
       61 gatgcctcaa ccgggaaggc cggatcgatg cctacgtggt ggaatccgag aacttcgaat
      121 ccatccacaa ccactccgcc tacgcgctga tcgaacacga caagcgcctc tgagcgcgtc
      181 gcgccggctt cgacgacccg gcgcaacggc caaaaaaaaa cggaacccga aggttccgtt
      241 tttcattccg gcgaccggat cgtcgatcag gcctgcgcct tggccgccgc agccttggcc
      301 gcgaaggact tgtgatccag cttttccttg atgcgcgcgg acttgccgga gcgctggcgg
      361 aggtagtaca gcttggcgcg acggacgtcg ccgcggcgct tcacctcgat gctggcgacc
      421 agcggggagt aggtctggaa ggtccgttcc acaccttcac cggaggagat cttgcgcacg
      481 atgaaggcgg agttcaggcc acggttgcgc ttcgcgatca ccacaccttc gtaggcctgg
      541 agacgctcgc ggttgccttc cttcaccttc acctggacca ccacggtgtc gccgggggcg
      601 aattcgggga tggtcttgcc ctgagtcaga cgggcgatct cttcctgttc cagctgctga
      661 atgatgttgc tcatgcgtca ctcctgaaaa atggccagtg gccttcgtgc agagcaggtc
      721 gccacctgat gcacggcttt gttatggaat ccggccaacg actatgccgg cttccgtcga
      781 gcctgccgga aagcttccag caggcgggat tcttccttgc tcaacacacc gcgccgggcc
      841 agcaggtccg gacggcgcac ctcggtccgc ccgagggact gctgcaggcg ccagcgccgg
      901 atctccgcgt ggttccccga cagcagcacg tcaggcaccg ccaggccttc gtacatcacc
      961 gggcgggtgt aatgggggca atccagaagt ccatccacga aggagtcctc gaccgccgaa
     1021 tcttcatcat tcaatgcgcc cggcaactgc cggatcatcg catcgagcaa caccatcgcc
     1081 ggcagttcgc cgcccgaaag cacgaaatcg cccagcgaca cctcttcatc cacacagcgt
     1141 tcgatgaggc gctgatccac cccctcgtaa cgcccgcaca acagtaccag ggccggctcg
     1201 gccaccaact ccagcacccg cgcatgatcc agcggacgcc cctggggcga caggtagatc
     1261 acccgccccg cctgcccggt caatccgacc tgccgcgcct tcgcagcggc gatcgccttt
     1321 tccagcggac cgggcagcat caccatgccg gggccgccgc catagggccg gtcgtccacc
     1381 gtgcggtaac ggtcttcggc gaaatcccgc ggattccagc acgtgagccc gtacaagccg
     1441 cgctcctggg cccgtccggt aatgccgcag ccggtcaggg cggcgaacat ttccgggaac
     1501 agggtgacga cgtcgtaggc ttttcccgcg ccggcagtca ccagtcggct tcccactcga
     1561 cacggatgcg cctggcggcc acatccacgt cggtcacata actggcgacg aagggaatca
     1621 ggcgttccac ctcgccgtcc tgcacctgca acacgtcatg ggcaccggtg gagagcaatc
     1681 cgctcacggt gccgagacgt acatcctgcg tattccacac ttcgaggccg atcagatcgg
     1741 cccagtaata ctcgtccttg tccggcttcg gcagggcttc ccgcggcacg gccacgtaga
     1801 agccatccac agcttcggcc gcattgcgat ccggcacctc ggcgaaggtc gccaccagat
     1861 cacccttgcc gtgggcgcgg caccccttga gggtgtaggg cgcccagtcc tggccgtccg
     1921 gatcggtgcc caggaaccac tggggcatct ttttccagga cagcggatcg tccccgaaag
     1981 gacggacccg cacccagccc gcaacgccat agggggcgac aatccgcccg agcacgatca
     2041 tcggcgcagc gcgagcgagg tcgaacgatc agccggctca ggcggccttg gcggcctgct
     2101 tgaccagacg ggcgacggtg tcggacagct gggcgccgtt gccggtccag tgggacagac
     2161 gctcgacgtt caccacgagg cccttttcgt tgccggaggc gaccgggttg tagtagccga
     2221 tgcgctcgat gaagcggcca tcacgacgat tgcgggaatc ggccgcgacg atgttgtaga
     2281 aaggacgctt cttggaaccg ctgcgggcga gacgaataac aaccatgact tgttcctgat
     2341 tccgttggat ggaaaaactc gcgattctaa atcaagacct tgccatgagc aaggtacgta
     2401 cggcgcgcag cccatgaaaa acggccggaa ggccctgccc tccggccgcg tcggggcgga
     2461 ttccgccccg ctccgcgcga agactcaatc gccgccgcgc atgtactgga cctggaacat
     2521 ggagggcggg ttgagcttgg gatcgacctg gcccttgaac tgtccggtgg tgcagtgctt
     2581 ggactgcacg tccaccatcg agaaggcgtc atcgatgccg atgccggcgc ccttgttgat
     2641 gggcaggtgg gcatccgggt gggacttgcc caccatgaag accttccaca gctcaccctt
     2701 gcggtcgtag atctcgttgg ccccgttcag gttgcccagc tgcgcgtcca tgtagaagac
     2761 gcgcttgctg atcgggtggt tgggattgac cggcgcggcc tccagcacat acaccttgcg
     2821 cagctgccag gtgccgttat ggaaacagcc gccctgcccg ccgaaatcca cgtacttgta
     2881 gccatccgcc tcggccggca tgtcgccggc gagcttcagc tcgttgtggt tccacatggg
     2941 caggatcacg ttcttggtgc ccttgtaggt ccacttcatg tccgagatgc gcccgttgta
     3001 gccctcgaag tcctcgatca tcaggtcgga gcccaggaag gcgtcggtcg tctgaccggt
     3061 ggccaggcgg cgcacgcggc gctggaagcc taggtacagg taggcatcgt ccagcttctg
     3121 gtcatcctcg tagcgctgga tgaggagctg ggtgttcttg agatcctgcg gatcgagggc
     3181 cttcacgtag atcgcccgga acagctcgga cgggttgggc gtcacctccg gcaccggcgc
     3241 gtccttgacc cggtgcttga agttcaggaa gtggaagttg aacttgatgg tcttctccac
     3301 ctgcccgctc tgcatgttgc ggtacttcca gtagaacggg tagatggccg cgccgtcacc
     3361 ccagttcacg ccgtacttgt agttccaggc gagcttctcg ccggcgcgcg ggtccttggc
     3421 gtccggctcc tcggggaagg ggcggccggc cacgtagcct tccaggtcgc cgttcttggg
     3481 tccgagtttg gccttgccca ggttcttgcg ggtggcctcc acgtaggcct tggagagctc
     3541 gaactgggtg gtcggtccga ccttgatctc gagcaggcct tccttgagca ggcgcagcac
     3601 gccggcgggg attgcgtcct tgaactgctc ggcgttgccc ttgttgatga cggtgcccgc
     3661 agtgagcccg gccgctttgg ggaagccgcc cttgtaggga ttgaagctgt tctccacgtc
     3721 ggcttcggtg gtggccagcg ccgtgccggc ggcaagggcg cccagcaggg cggcgatgag
     3781 tgtcttgtgc atggtgtctc ctcgatcaga acttgtagcg ggccatcagg aagatttcgt
     3841 tctcggccat ggcggtgccg atggggccgg cgcggaagcg gcccaggggc tcgtagccgc
     3901 ccagcccgta ggagccggcg ccggtctgcc cgggataggc ggtaaagggg ttgtaggggt
     3961 tacaggaacg gcagtcgtca aactggttgc ggctgttgga gccgcccttc aggttggcgc
     4021 cgaaggtgag cttgaggtcg ttggagacca gccagtccag ggatggggcc acggtctggg
     4081 cgcgggcgcg gaagtcgcgg gccaggatga cctgcgggct cacccggtcg ccgtgcatga
     4141 agcccttcat cagcagggtg ccgatcacgt tgtcttccca gtcgggcatg cccaccgggc
     4201 cgtaggcgcc ctggcgcagt tcgtggtcga agatgtgctg gtagaagagc tgggcggaga
     4261 acagagtggt gcggtgcggg ttgatccagg gcacgaaggt cgggcggtcc atgccgatca
     4321 cactgcgcca cacgcggttc ttggaataca gctcggggcg cgcggtgttg gcgaactctt
     4381 cgccggtggt gagggcacct tcgagccgga aggccgcgcc cagggtctcg gactgcagat
     4441 ccagggagcc accgatcagg ttcacccgcg ggaagtacat gtcgaaggcg atcaggtggc
     4501 tggtggggtc cgggttcacg ccggtgaagg ggttctgggc gctgttgaag gcgcgcaggg
     4561 agggcagctg ggaacggtag gtgaggccgt tcagggagaa gctcaggccg tccttggtga
     4621 tgccctcgaa cttcacgccc agttgggtgt tgttgaagct ccaggcgggc agattaacgt
     4681 tgcgaatgcc aatctggccg gggccgaagt tggtggcgag ggtgccgccg gcaaagttgg
     4741 ccacggtgcc accggtgtcc cacaggccct tcatgccgcg gaagaagcag ccggcgtcga
     4801 ggatcacgtt gggggtgccg cactggccca ggttgttggc gcggaactgg tcgaagttcc
     4861 acaccacctg caggttgcgg tcctgcatgg attcgctggc gcccatccgc cattcggtct
     4921 ggaggatcca catggggatg cggatgtcct gcagctcgtc atagatgttg ttgcgcgagt
     4981 agtcgacggg gttgatcacg tcgagcacgc ggaacaggtc ggtgcgcccc cacaccacct
     5041 gctgcttgcc gaccttgacg aagagctcgg tgccgtcaga gagggtgaag gagttgcggg
     5101 cgtagagctc gcggatgaag tcgagacggc tgttgaactc gggcagttcc agttccgaga
     5161 gggtcttgtt gccgtagcca ccgaagttca cgcagccccg gttgtcgtag tcgcagggac
     5221 gcaccggcac accgaaagcg acgccaccgg cctgggcatg ccagcggtcg cccaagacgc
     5281 gcaggccgtc gttgggattg cccgggtagc tgtccacgaa ggcgttgttg gtgaggccga
     5341 aggcgccggc caccgcggtg tggccgaggc cgccaccctg gggcaccatg gtgggcatgc
     5401 ccaccgtggc accgcctgtg ttctccagca ggatggggcc gccggcgtcc ttgccgtatt
     5461 cactggaatt gaggcgatac acgccgtccc aggtgccgcg caggatgctg tggatgttcc
     5521 aaccgttgcc ggcgttcttg tcggcctcca cctgcagggt gttgcggaac ttggacaggc
     5581 cgacgctgcg cccggtgttg tccttgccgc ggccggcggt gtggttctcg taataggtgt
     5641 ccacgcgcca gggttcggcg ccgccgccgc ccaggtcggg cagctcgtcg gccagcaccg
     5701 gcacggcgaa ggccgccgag acggccaggg cacacaggct gagggccggc tggaaccggc
     5761 ttgatctgtt cttcatgagt gtctcctcct tggttgtacg atttgcggcc acctgcgcgg
     5821 ccgccctccc ttcaaatcgt gtgatctcta tgcgcgcgcg tctcctctag tcggcttcca
     5881 ggactccgtc ctcgtcgtag tggatcgccg tgacgaactt gggcttgaac accacgcacc
     5941 aggagggaac gatgagcacc gccgcgatgg cgttgacgat gagcatgaag gacagcagca
     6001 cggcggcatc ggactggaag cgcaggtcgg acatgaagat ccacatgacg acgccggcga
     6061 tgagggtggc ggcggtgaag ctgacggcgt agccggtggt ggccacggcg cggatcacgg
     6121 cctgtttgat gtcgtgcagg gcggcgtact cttcgcggat gcggtccatg aaatacaccg
     6181 cgtagtcgat gcccacgccg acgcccacgg cgatgacggg caccgtgttc acgttgatgc
     6241 cgatatgggc ggcgcccatg taggcgtagg tcatcagcgt ggcgaagagc atgggcagga
     6301 ccatcagcca gccggcatgc agggagctgt aatagaccat caccagcacg aaggcgatga
     6361 gcatcacggt ggggatgatg atcaggtggt cggtgtgcag ggcctggttg ccggcggcgg
     6421 tgacgccgac gatgccgccc cccagttcca cgtggatgcc cttgacctcg gcttcgagct
     6481 gccgtttgcc cgcttcggcc agggcgacga cgcgccggat ggtctcggcc tggtggtcct
     6541 tgtagtaaaa gaccatgtcg gcttcgttct cgtcggcgtt cacgaactcc ttcagggcgc
     6601 cgggaatggg gctggcggcc atgtaggcga acatcaggcc gcccacttcg tcggcggtgt
     6661 cgggcacctg catccagcgc gggtcgtcgt tgtgggtgag cttgttcacc acgcggatga
     6721 cgccgggcag ggccttggcg ccgcccaggg tggggtcgga aagcatgtgg gcctggaagc
     6781 gctcgatggc ggccatcacc tcggggcgct tgatgccgcc cttctcgtcg gtgcgcatca
     6841 gcacgtggag ctcttcggaa cccgggaagc gggtgttgat ggcccgcgtg gagacgttgt
     6901 agtcgtggtg ggtgtggagc agcggggagc cgggttcggc ttcgccaagc tggacctggc
     6961 cgacgaagta cagcccgccg acggcaccga tgagggtgag cagcaggatg ctgcgggcgc
     7021 cgccgtcggt gccgccggtg cgcgccatgg cggcaccgag gaagttgcgc acggcctcgt
     7081 tggcgttgac cgtgttcttc ggagcgggca gcacggtgag ggccagggga accatgaagt
     7141 gcacggtgaa gatcaccgag aagccccaga agccggcggc gaggccgagc ttgtggttga
     7201 agggggctgc gccgaggacg agcacggcaa tgcccacggc gtccacgatg atggccagcg
     7261 agccgggccg gaacagggca tcaagggcgt tgcgggcggc cttccggcca tccttcacga
     7321 tggccagctc ctcgtagtaa cgcaccacga gctgcacgcc gtgggaggtg gcgcgggcgg
     7381 cgatgaggaa ggggatgggc agggacaggg gttcgaggtt gatgcccatg agggccatga
     7441 aacccaggcc ccagatcgtg gacagggcga tgcccagcag cggcagggcg atgccgtaga
     7501 ggcgccggaa atagatcacc aacagaaagg tgagcagcgc gagggtccag gcgaggatct
     7561 cgacgatctg gttgaggtag gtgtacaccc agccggtgag cacggggttg ccggtgacgt
     7621 agagggcgtg gccgggggtg gcgagggatt ggcggacctc ctgcagggcg gcgaaggtct
     7681 tggggtagtc gatgtcgcct tcgacgagct gggccttgat gagggcggcc ttgagatccg
     7741 gggagacgag caggccgtag atggtggggt tggcaatgac gtccttcttg agctgctcga
     7801 gctcggccac ggtgcgcccg ctcttcatgg ggtcgtagta ggactcgctg gcgaagccgc
     7861 cgctctcgtc gagaaagatc ttgcgggcag tgcggtgggt gaggctgccc acgaggttgt
     7921 ggttcacgcc gggcaggctg tcgatgccct gggtggcctt gtggatgagg gcgagggttt
     7981 cgtcgttgaa gatcgtgccc ttctcgacct cgatggccat cacgatcatg ttggcgccgc
     8041 cgaagttctc cttgaggcgg ttgtagacct tgatgtaggg gtggttctgg ggcagcaggt
     8101 cggcgaagtc ggagtagatg cgcagcttgg gcagggcgac gccgaacagg gcggtgacga
     8161 tgaggatggc ggcgaggacg atcttcgggt tggcgaagag ccattcctcg gcgcggtgca
     8221 gggtgtgggt gatgcggtga cggaactggc tgaccatgat ggggcctcag cgagccgcga
     8281 gggcctgggt gccgatgacg cgcagcagcc cgcccgggcc gccgatgaca agggcgtgtt
     8341 gttcgggaag ggacgcggcg gcgccgagga agacgggcag cggatccgga taggcgacgg
     8401 gttgccactg gccaccatcg aggcgggcga cggtgccgcc ggcgccgacg ccgtagggaa
     8461 tgccctcgtg caggaagagg cggtagaggg gcatctggct ggcgttgggc tggcgggtcc
     8521 agctgaggcc gccgtcggtg gtgtgcagga tctggccggc gaggccgctg acccagcctt
     8581 cggcggggct cacaaagagg ctggcgtagg ggtagaactc gtcgggcatc ttgccgcgcc
     8641 ggctccaggt ggcgccgcca tcggtggtga cggcaatgag gccgaattcg ccggtggcga
     8701 tgccgaagcg ggcgtcgacc atctgcacac ccgtgagctg ggcgtcttcg gccagttcgc
     8761 tgacctgcca ggtggcgccc cggtcggcgc tcacggcaag cgtggcggcg gtgccggcga
     8821 cccaccagcg gccctgggca tcacagctga cggcgagcgg ggtgcggggt ttgtcgaggg
     8881 ggacggcgct ccattgggtg ccggcggcgt cggcacgcca gactttgtgg aaaaagtcga
     8941 cgcccacgaa gctgccgtcc gggcaggcgg ccagccccac cagggaggcg ccggcgagca
     9001 tctggcgctg ccagctgcgg ccctggtcgg cggagacgag cacagccccg ctctgggtgc
     9061 cggctaccac ggtccggccg ttggccgcca gggcctgggt gatgtcgtaa cgctgcaccg
     9121 ggcgcgcggc ttcggcgttc acggcggaaa gatcggcggc gctctggcag ccggtgagcc
     9181 ctgcaagggc cagacaggcc atcagggcca cgttcagcgc ggtccgcgca cagcgcttgc
     9241 ctgcgtgttt cacctgggtc tcctccagtt tttatgtttt tttgggacaa accgccccct
     9301 ccggcgggct ctggcccacc ggcttgatcc gatccggccc gcccgctcga cgggcgggcg
     9361 cgcgggcggg gccgctagcg gccgaggtcc ttggcgctga cgccggcgat gccccagttg
     9421 gtcttgggca cgtcgtggat ccacacgcgg acgttctcct tgggggcccc caccgcctcc
     9481 accagggcct gggtcacctt ttcgatcacg gcgcgcttct gttcctcggt acggccttcg
     9541 atcatgtaga tctgcgcaaa tggcatgtcg ctctcccgtt tattgggtga cttacttgaa
     9601 gcggagggac accgaaccca tgccctgcac ccgcagggtc acgtggtcgc cggcctggac
     9661 cgcgaccgcc tcggtgatgc cgccggacag gatcagggcg ccggcgggga tctcctcgcc
     9721 gcgggcgccc aggtggttgg ccagcatggc gatggccgcc gccggatggc ccagcacggc
     9781 ggcgccggcg ccgaaggcca ccggctcgcc gttcttttcc atgacgatgc cgacggtgcg
     9841 cagatccacc tcgctcaccg gcaggggctg gccgcccacc acatagcggg ccgccgaggt
     9901 gttgtcggcg atcacgctct tcaggtcgaa cttgaagtcc cggtagcggc tgtcgatgac
     9961 ttccatgccc ggaatcacga agtccgtggc agcgagcacg gcgccgatgt ggcagccggg
    10021 ccccttgagg gcctgcttca tgacgaaggc gatctccggc tccaccttgg gatggatcag
    10081 ctccgacacc ttcacttcgc cgccgtcagg gatcgcgccg tagtcggtga tgaagccgaa
    10141 gcacggcgtc tccacgccca tctgcttcat cttggcgtag gaggtgagcc ccgccttgag
    10201 gccggtgatg cgggtgccgc gggcgcgctt gcgggcgcgg atggcgtcct ggatggcgta
    10261 ggcgtcttcc cagtccatgt ccgggaattc gtcggtgatc ttcagcgtgt cgcgggcctg
    10321 gagctcgcag tcctccaggt gttcggcgag acgggcgatg gtggcctggt cgagtttcat
    10381 accgtcagcc cccgcgcctt ggccatggtg atggccgtgt cctcgatcat gtcttcctga
    10441 ccgcccacca tgccgcggcg gcccagctcc accaggatct cccgcgccgg cacgccgtac
    10501 ttgaccgcgg cacgcttggc aaagagcagg aaggagccat acacgccggc gtagcccagg
    10561 gtcagggcgt cccggtcgat acggatcggg aaatccatga tggggaagac caggtcttcg
    10621 gccacatcgg tgatcttcgc cacatccacg ccggtggcga tgcccatcag gtcggccacg
    10681 gcgcagaaca cctccatcgg cgtgttgccg gccccggcgc ccaggcctgc ggcggcggcg
    10741 tcgatgcgcg tggcgcccac ttcgatggcg gcaatggaat tggccacgcc catggccagg
    10801 ttgtggtggc cgtggaagcc cagctcggtc tccggcttga gggcctcgcg cacggcaccc
    10861 aggcgggcct tgacgccgtc gggcagcagg tggccggcgg agtcggtgat gtagatgcag
    10921 ttggcgccgt agccttccat cagcctggcc tgcttgacca gaccttccgg gctgttcatg
    10981 tggctcatca tcaggaagcc gacggtgtcc atctcgagct tgcgcgccag ggtgatgtgc
    11041 tgctcggaga cgtctgcctc ggtgcagtgg gtggccacgc ggatggtgtg cacgcccagg
    11101 tcgcgggcca tcttcaggtg ctcgacggtg ccgatgcccg gcagcagcag ggccgacacc
    11161 ttggcctgct tcatcttcgg aatcacggca cccaggtatt cctcgtccgt gtgggcgggg
    11221 aaaccgtagt tcaccgagga gccgcccagg ccgtcgccgt gggtgacttc gatcaggggc
    11281 acgccggccg cgtccaggcc ggtggcgatg gcgaccatct ggtcgagggt catctggtgg
    11341 cgcttggggt gcatcccgtc gcgcagggtc atgtcgtgga cggtgacttt cttgccttgg
    11401 aggttcatgg tgcgggtcct cgcgggttaa tgggcgctgg ctgcggcctt gggttcgagc
    11461 acgaggcggc cggcgaggat ctcttcggcg aacatctccg cggtgcgggc accggcggcg
    11521 gtcatgatgt ccaggttgcc ggcgtacttg ggcaggtagt cgcccagccc ttccacttcc
    11581 agatagacgg agacgcggtt gccgtcgaag accgggccgt tcaccaggcg atagccgggc
    11641 acgtacttct gcacttcgcg gatcatggcg tggatggagg cggtgatctc gtcgcgcttc
    11701 ggctcgccct cgacgaggca atgcacggtg tcgcgcatca tcagcggcgg ctcggccggg
    11761 ttgatgatga tgatggcctt gcccttctcg gcgccgccca ccttctcgac ggcacccgcg
    11821 gtggtgcggg tgaactcgtc gatgttcttg cgcgtgccgg ggccggccga tttggacgag
    11881 acggtggcga cgatctcgcc atagctcact ttctgcacgc gggaaacggc ggcaaccatc
    11941 gggatggtgg cctggccacc gcaggtgacc atgttcacgt tcatctcacc cttgccgacg
    12001 tgcgccagca ggttcaccgg cggcacgcag tacgggccga tggcggccgg cgtgaggtcg
    12061 atcatgagca cgcccagcgc gttgagcttg cggctgtttt ccgcatgcac gtaggccgag
    12121 gtggcgtcga aggcgatctg cacgccatcg gccagcacgt ggggcagcag gccgtccacg
    12181 ccgtcggcgg tggtcttcag gcccatctcg cgggcgcgct tgaggccgtc ggagtcgggg
    12241 tcgataccca ccatccagac cggttcgagg acggggctgc gttgcagctt ggccagcagg
    12301 tcggtcccga tgttgccggg gccgatcagg gcacacttga ttttcttgct catcgccggg
    12361 attcctttga ggtcgcgaag ggttaaacca tgctgcgcac cacgccgcct tcgacccgca
    12421 gggccgcgcc gtgggtggca gatgcaaggg gactgcagac gtaggcgacc agggccgcca
    12481 cttccgcggg ttcgatgaaa cgcttgagca gcgaggtggg ccgcgcgtgg gcgaagaagt
    12541 cccgctcggc ctgctcgacg gacacgccct gctcgtccgc caggcgcccg aagaaggtcg
    12601 ccacgccttc ggtgcgggtc gggccgggga gcaccgaatt caccgtcacg ccggagccgg
    12661 tgcaggtttc cgccagcccg cgggccaccg agatctgggc cgccttggac atgccgtaat
    12721 ggaccatctc ggccggcgga cacacgccgg attcactgga gatgaagacg atgcggcccc
    12781 agtcgcgggc cttcatggcc ggcaggtagt ggcggcacag acgcacgccg ctcatcacgt
    12841 tggtctcgaa gaaatggaac cactccaggt cgggaatgga ctcgaagggc ttcgggtcga
    12901 agatgcccag gttattcacc agcacatcca cgtccgggca ggccgccgtg agggcctcgc
    12961 agccggtggc ggaggacagg tccgccgcca cgccgctcac cttggcgtcg ggcacgaccg
    13021 cctgcaggcg ggccaccgcg tcggccacac cggcccggct gcggccgttc acgatgacct
    13081 cggcgccctc ctgtgccagg gcgcgggcgg tggcgaagcc gatgccggcg gtggagccgg
    13141 tgacgagggc gcgcttgccg ttcaggtgca gatccatgtt cgcgatctca gctgaagcgg
    13201 acggagcagc cgccaatgcc cccgatggtg acgcgcaggc tgtcaccggc cttgaccggg
    13261 atcagcgcag ccagcgagcc ggacagcacg atgtcaccag ccttcaggcc gatgcccagg
    13321 cgcccgaggg tgttggcgag ccaggccatg gcgttgaggg gatggccgag ggccgccgca
    13381 ccggcaccgg tgccgacgat gtcaccgttc ttctccagca ccatgccgca ggtggcgaga
    13441 tccaccttgc gcggatcgac gatgcggtcg cccagcacca gcacgccgca ggaggcgttg
    13501 tccgcgacgg tgtcctggat cttgatcttc cagtcgtgga tgcgggaatc gacgatctcg
    13561 aagcaggcca tcaccccttc ggtggcagcc aggatgtcgg cgccggacag gcccggaccc
    13621 atcacgtcct tcttcatcag gaaggcgatt tccccttcgg cgcggggctg gatcagggtc
    13681 gcggcaggaa tcgcctggcc ctcgttgtag accatgccgt ccagcagcca gccgaagtcg
    13741 ggctggtgca cgcccagcat gttcatcacc gccttggagg tgacgccgat cttcttgccc
    13801 acggggcgct cgccggcggc gaggcggcgg gcgatcatct gctgctggat ctggtaggcg
    13861 tcctcgatgg tgatgtcggg gtggcgctcg gtgaggggcg cgagggtgcg acgttggatg
    13921 agggcgtcgt agagttcgtc gccgagttgc gtgatcaggg ccttgtccat ggcggggtct
    13981 caggaaaggg gttggggttc gtcgtccgcc gcttcggcca ggaaatcgcc gacgaggcgg
    14041 gcgaagcggg cggcgtgttc gatctgcgtc cagtggccgc actggccgaa gacgtggagc
    14101 tgggcgcggg gaatccaggt ggccagggtc agggagttgg ccagggggat cacctggtcc
    14161 tcgcgaccgt ggatcaccag ggtctggttg ggcagcgcgc ggatgtccgc ctcggcgctg
    14221 gtcatggcgt ccacccagcg ctggcgcggg gccgggaaca tggcaccgaa ggattcctgg
    14281 aagccggggc gtacgctggc ctcgtagcgc aggcgggcga gttcgtcgtt caccagcccg
    14341 cggtcgtagg cgaacaggtc caggaggcgg cgcatggcct cgatggacgg ggcatagccc
    14401 cacaccgcgt cgagggccgg ggtgatctcg aagggcacgc ccacgctgcc catcagcacc
    14461 agccggcgca cccgctcggg atgctggatg gccagggcca gggcgatggc accgccgaag
    14521 gagttgccca ccaggtcagt ctgttcgatc tccagtgcgt ccagcacgcc gagggcgtgg
    14581 ccgacccagc cctgcatgtc gtaggactcg ccgtcgaagc gttcggtgaa accgaagccg
    14641 gccatgtccg gcgccaccac gcgccgtcgc tcggccaggg ctggcatcac cagccgccag
    14701 ttggcccacg cggagacgcc cggcccggag ccgtggatca gcgtgacggg cgcgccggag
    14761 ccctggtcgt ggacgttggt ctggcggccg gcggcgcgga gcgtgcggcc gatttccggg
    14821 gaggcggtgg acatggcggc ggtccgggtc acagcttgat gcagatgttc ttgagctcgg
    14881 tgtagaactc cagggagtgc accccgccct cgcgaccgat gccggactgc ttggcaccgc
    14941 cgaagggggt gcgcaggtcg cgcaggaacc aggagttcac ccacaccagg cccgtctcca
    15001 gcttgccggc gacgcgatgg gcgcgggtca cgtcacgggt ccataccgac gcggccagcc
    15061 cgtaggtggt ggcattggcg cgacgcacga cctcgtcctc gctgtcgaag ggcatcacca
    15121 gggtgcaggg cccgaagatc tcctcgcggg cgatggccgc gtcgtccccc aggccggtcc
    15181 agatggtggg ctgcacccag gcgccgtcct tcagctcggc cggcacctcg ggcaccccgc
    15241 cgccggtgac gacggtggcg ccctcctcca ccgccttgcg gtagtagccg agcaccttct
    15301 gcttgtgctc ctgggagatc agcgggccca tgccggtttc ggcgctgtcc gggtggccga
    15361 tcaccaggga ctcggcgccc tgcttgaggc gcgccacgaa ggtgtcgaag agcgggcgct
    15421 ccacataaac ccgctcggtg cccaggcaca cctggccgca gttggcgaag caggaacgca
    15481 gggtgccttc gatggcggcg tccaggtccg cgtcggcgaa gacgatggcc ggattcttgc
    15541 cgcccatctc cagggacacc ggccgcgcgc cgtccgccgc cgcgcgcatg atggcggcgc
    15601 cggtgcgggt ctcaccggtg aaggtgatgg cattgacgcc gggatgggtg gtcaggaatt
    15661 cgcccgcgga tccgggacca aagccgtgga ccacgttgta cacgcccctg gggatgccca
    15721 cggcgttcat cacttcgccc agcagcgtgg cggtctgggg cgtctcctcg gaaggcttga
    15781 ccaccaccgc gttgccgcag gccagggccg gaccgacctt ccaggtcatc agcagcaggg
    15841 gcaggttcca cggacacacc accgccacca cgcccaccgg ccggcggatc gcgtagttca
    15901 gcgcgccgcg gccgtccggg gtggccatct cgaagaactc cgtggggacg ttcttcacca
    15961 cgtcggcaaa gatcttgaag ttggcggcgc cgcggggaat gtccaggtga cgggcgaggc
    16021 tgcgcggctt gccggtgtca gccacctcgg cggacaggaa gtcttcgaag cggcggttga
    16081 tctcgttggc cacgccgtag agcaggtcga cccgctccgc cacggtgatc tggccccacg
    16141 gaccttccag ggcggcgcgg gcggcaccga cggcggcgtc cacctcgggc cggccggctt
    16201 cgctgacctg ggcgatgacg cggttgtcca ggggcgaatg cttgtcgaac aggcgcccgc
    16261 tggcggcctc gacgaattcg ccgttgatga agtgcttgat ccggttcatc tgttgttctt
    16321 ccttcgtggg tgaagtgctg ttcattcggc gccgagggcc gcgagggcgg cccgggcgca
    16381 gtcttcgtcc tgggcttccg aggcaccgga cacaccgatg ccgccgatca gctcgccgcc
    16441 gacgcggatc ggcagcccgc cgccgaacac caccaggcgc ggacgggccg agaagccgag
    16501 cttcatgcct tcgtcgtggc cgatcagctt catccagtcc ttggtggaaa agccgaagcc
    16561 ggcggccgtg taggccttgt cgatggcgat gtcgatggat tgcaggaagg cgccgggcat
    16621 gcgcaggaag cccgccaggt tgccgccgct gtccgccacc gccacgttga tccgtacgcc
    16681 cagttccatc gcccgggaca cggccgcctg cagggcgacg acgctcgctt cggctgagat
    16741 cacggactgg gtcacggcga cgggttcggg gcgggtggcg ttcatggtcg ggtttcggcg
    16801 tcttgttggt tgaacgatat ttccattatt tctcgacgtc aactattttt ctcgagattt
    16861 tgaattattc ttgtgggaaa cggtttggga taaaattcac cggccccagg ttcgcccctt
    16921 ccggccggac acacaccaag gcgagagacc atgagcgaac atcccagcat ccaggacacg
    16981 ggcagcagcc ccgacgacga acccaagacc ctggccgagg cggcctaccg gcaactgcgc
    17041 cgggacatca tcgaaggcgt
//
LOCUS       DAOQMM010000081        16870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1296_length_16870_cov_45.688671, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000081 DAOQMM010000000
VERSION     DAOQMM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16870
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1296_length_16870_cov_45.688671"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..1459)
                     /locus_tag="PLH85_12585"
     CDS             complement(308..1459)
                     /locus_tag="PLH85_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB65449.1"
                     /translation="MNGLFSAAGLRRLRVMTRKELIQLFRDVALMVFFVYAFTGDIYI
                     AASGVSMQLKNAALAVLDGDASFASRELSSRFQPPYFRVAGAAGTPAQSIRHLDNADV
                     MALLDIPPRFEETLARGGQAAVQLQVDTSNSVLGFLAASYGAQIVGKYALETAALREG
                     LGSGGVAGPTLAAETRVWYNPNQNDAWFMGISELLTVITLFSILLPAAAMVREKERGT
                     IEQLMVTPLSAFQILCPKVVAMTLVILAGTAIALFGILVPCFDVPMRGSLVSFFLITA
                     LYVFTTAGLGLLAATVARNLAQVGMLTVVILMPMLLLSGAWTPIEAMPEWMHLLAWIS
                     PLHYFLDAAYGILLKGAGLDLLWPSVLSMAALGVSVAALGMGRFGRQFG"
     gene            complement(1456..2610)
                     /locus_tag="PLH85_12590"
     CDS             complement(1456..2610)
                     /locus_tag="PLH85_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB65450.1"
                     /translation="MNGRRIVSLAWKEWREIVRDRLFFALAFFVPTLLMVLFGFGLTL
                     DVENLPFAVVDQDRSAASRDYAYRFVSSRYFDFKGQVEDVRQLDRLLADNSIRLALVI
                     PPRFGEHLARGEPAPVQVLIDGTFPMRAQTTQGYVAAIHSAVSLDSMAQALSALRGIP
                     LAEAQTRVQPVKLEVRYLYNQSVKSIWSLAPKLIMLIMMLSPPFLTALGVVREKESGS
                     IFNIYASTVSPGEFLIGKLAPYVLISFINGLILWALAIWLYGTPFKGDPLFFALALLL
                     YVVCTTGIGLVISMLVQTQVAAMIVASIVSIIPAVLYSGMLLPLSSLAPSALAIAHAL
                     PPMYFTNIVVGSFLKGVGWGALWGDVLVLAAYALGLFGIGYLLFTKRPKS"
     gene            complement(2607..4583)
                     /locus_tag="PLH85_12595"
     CDS             complement(2607..4583)
                     /locus_tag="PLH85_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB65451.1"
                     /translation="MNAPAIRVEGFVKRYGRRVAADGLTLTVQPGEIYGLVGADGAGK
                     SSLMKAVAGVLAYEGGRVEVFGLPVDSERAAERVKQRIGFLPQGLGLNLYPDLSIEEN
                     VDFFGRLRGVSAAQLAERKTLLLAMTRLDAFRARPMRQLSGGMKQKLGLVCTLIHEPD
                     LVILDEPTTGVDPVSRRDFWAILGRLLEEKGITALVSTAYLDEADRFHRVSLFHEGKV
                     MGEGTPAALVATVPGTLALFRAEPQAEALPRLKARFPQTEAMGAWFRVFAEGLDADAA
                     RGAVAACLDIAPAATTTGTAAPTIDLRDWFAREPELEDVFVALLRRRGAQLDSRVEGA
                     TRRAAAPGELAIEARELTRVFGEFRAADAVSFAVPQGEIFGLLGANGAGKTTVIKMLT
                     GILKPSSGAGRVAGADMRTAGLAIKERIGYMSQAFSLYHDLSVVENIRLYAGIYGLDD
                     RQAQRRTDWVIGMAGLAGFEHDGAGRLPMGLRQRLALGCALVHEPRVLFLDEPTSGVD
                     PIGRRMFWDILFHLSRREGVAILVTTHYMSEAEHCDHLALMYAGRVVADASPEAMKAA
                     IAEESGRLLELRVDRPVPAQAALKAAGYGSAALHGRAVHLLSRDVDGDLARLPGVLAA
                     AGVQVQAAGLRPLSMEDVFVQRITALEEAARRAP"
     gene            complement(4591..5658)
                     /locus_tag="PLH85_12600"
     CDS             complement(4591..5658)
                     /locus_tag="PLH85_12600"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB65452.1"
                     /translation="MNILTRRIGGAVAVVAVLAAGGWAWHEWAGSAALPEGLIQANGR
                     IEGDRTTVASKLAGRIAALKVREGDSVQAGQVLARLEDTQLAARQQQADAAVATLQAQ
                     VAAAQAQLAVARREVPLAEASADAQLAKARAADAQAAKDAARFDELAERGTVEVRRAE
                     QMRLVRSSAAADLRQAEQALASAHLGHDKVKARENDVAALEAGLHQAQAAAKEVASLI
                     DDLSLRAPVGGVVLNRLRESGEMVAAGTPVFEIVDLDRLYLKVYVPEKLIGQLRLGLP
                     ARVYSDAFPAQAFAATTRYIASRAEFTPKEVQTPDERVKLTYAVKLYLDANPEHRLAP
                     GVPADAVIRWKDEVPWQAPRW"
     gene            complement(5727..7031)
                     /locus_tag="PLH85_12605"
     CDS             complement(5727..7031)
                     /locus_tag="PLH85_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB65453.1"
                     /translation="MRFRPLSIALSVLLLSAAPTGWAAETLADAWAQALSVDARLRAA
                     DAEGEGARATLEAAKGLALPKATASSRYTVLSKEPAALIGLPTIPGISLPDRMPLSER
                     TYATHALQASLPIYTGGRIAAAVAAADIGVQASAQERLRTEQTVKLDVAEAWLNVLRS
                     RRALAAAERHVAALDAHAADVNALHRQGVVPRNDVLSVAVALADARQQQTRARHGVDL
                     AAAAYNRLLGRPLETAVDVHEPPPPDAAREDFSTLVARARAARPELALLGARAEATDR
                     EADMERAARRPQVGVNAAVMHEDNRYRASQDIAQVAVGVQWELFDGGIQRQRADAAAA
                     RARAARERQADTATLIELEVRREWLAVEEAAQRLATTGAAEAQADENLRVARDRYRNG
                     VGTHTEVLDAEALRALTDSNHLNAHYDLHGARYRLKKAVGEL"
     gene            7384..7872
                     /locus_tag="PLH85_12610"
     CDS             7384..7872
                     /locus_tag="PLH85_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65454.1"
                     /translation="MPLPALDDDLVDFGDIPAPINALLQEGVYWHPADPARARACFRS
                     AIDLAPDVLPSYRCLFKAHNKQRDFAAAYAVALEGLGRAAMQAGMSPDWSTWTRPALA
                     AAPTVPARFLLHSLKAVAFIELRRGHESASRAALAHLQALDPEDGVGASVIAALLDRE
                     DA"
     gene            7872..8204
                     /locus_tag="PLH85_12615"
     CDS             7872..8204
                     /locus_tag="PLH85_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65455.1"
                     /translation="MRRESDTPPFAVTQTVHRALGPLVVLEVGALKVRLTPREAHTLA
                     LALFAVRDGRSAETELFMSPIASDGIFVAPVGSDGIAVETSAGPVALDWQAVGELGAQ
                     LSPAAVPG"
     gene            8401..10767
                     /gene="parC"
                     /locus_tag="PLH85_12620"
     CDS             8401..10767
                     /gene="parC"
                     /locus_tag="PLH85_12620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNB65456.1"
                     /translation="MNDTPDLFDTLPPDTPEPPPPPVATPLAGSDDALPLDLYAERAY
                     LSYAMSVVKSRALPQVEDGLKPVQRRILFAMNEMRLSPASKHVKSARVVGDVIGKYHP
                     HGDSSVYDAMVRVAQDFSLRYPLVDGQGNFGSRDGDSAAAMRYTECRLTPIAELLLSE
                     IDRGTVDFRPNYDGAFQEPQLLPARLPFVLLNGASGIAVGMATEIPSHNLREVARAAI
                     HAIEHPEATVADLLGHIAGPDFPGGGQLISPAADIRQAYETGRGSLKLRARWIVEDLA
                     RGQWQLVITELPHGVSAQKILFEIETLTNPQPKTGKKSIDPDQAQLKALILSALDRVR
                     DESGKDAPVRLVFEPKSRNQDVAEFANLLLAHTSLETSVALNLVMIGLDGRPAQKNLA
                     DILHEWGRFRTVTVRRRSVHRLGQVDDRIHILEGRHIVFLNIDEVIRLIRESDDPKAA
                     LIARFDLSDRQAEDILEIRLRQLAKLEGIKIERELAELRGEKTELEALLADEGKLRRL
                     VIKEIRADAEKYGDARRTLVEEAARAGVTQTVVDEPVTVIVSEKGWVRARSGHGLDLA
                     NVAFKDGDRLATALECRTVDALIALTDGGRALTVAVSTLPDGRGMGAPLASFVELPAG
                     AKIVHVLSGDPASRVLMATRDGYGFLCRLGDMLATKKAGKQFVTLDEGVGLLPPLIYA
                     DAPDLHVATLSEKGRLLVFTLDQVKELSGGGRGVILQALEDGDTLRAVCVAGSDGIVL
                     SSASSAGRAYEVPIPARDWTPWLNKRARKGKPAPGRFTPTAVTPLKRD"
     gene            complement(10754..11299)
                     /locus_tag="PLH85_12625"
     CDS             complement(10754..11299)
                     /locus_tag="PLH85_12625"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB65457.1"
                     /translation="MAAELTETCVRSEQVFDGVLLNVHRDTVRLPDGKQGVREYIRHP
                     GAVVVIAQLPDGHLIFERQFRYPCGRVFIELPAGKIDAGEDIERCARRELLEETGYVA
                     DSWRHLGVMHPCIGYSDERIEIFLASGLRHEGASLDDGEYLEIFPMSLDEAEAAVLDG
                     RITDAKTITALFWAAKVLSPA"
     gene            complement(11407..12591)
                     /locus_tag="PLH85_12630"
     CDS             complement(11407..12591)
                     /locus_tag="PLH85_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HNB65458.1"
                     /translation="MASRDVVVLSAVRSAIGAFGGSLADFDASELAGIVMKESVVRSG
                     VDPQLINYVTVGNCIPTDARYAYVSRVASIQAGLPMESVAMQVSRLCSSGLQGIVTTA
                     QNILLGDADYGIGGGVEVMSKATYMLPALRSGARMGDTKAIDSMVAVLTDPFGVGHMG
                     ITAENLAVKHGISREEQDAFAVESQRRAAVAIDEGRFKSQIVPIVKQTRKGDVVFDTD
                     EHVKRGTTLESLAKMKPAFKKDGTVTAGNASGINDGAAFFVLADGATAVNAGYKPMAR
                     LVSYAIAGVPNDVMGEGPIPASKLALKKAGLTLDQMDVIESNEAFAAQALTVMRGLGL
                     DPVKTNPNGGAIALGHPVGCSGAFIATKALYELQRTGGRYALVTMCIGGGQGIATVFE
                     RI"
     gene            complement(12618..13547)
                     /locus_tag="PLH85_12635"
     CDS             complement(12618..13547)
                     /locus_tag="PLH85_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HNB65459.1"
                     /translation="MTLFSLIVALLIEHLRPISTERFVEAPLKAFAHFLEDRFNDGEA
                     RHGAVAWWVAMLSVGFVSALAYFVLWHVHPLLAMVFNVVILYFTMAFRNLSRFFSDLQ
                     LALRMGELARARQLIGVWRKRSHDASSSSEVARLAIEEALIAAHRNVFAVIFWFILLP
                     GPSGALLYRLARFFDEEWSLRKGEPEFGDFGEFAHRAFAALDWLPVRLSAAAFSIVGD
                     FEDAVYCWRAQAAQWADKASGILLASGGGALGVRLGMPVHESGEIVERPEMGIGDEAD
                     ADFMQSTIGLIWRTLVLVFLVLALLGVAGWVGH"
     gene            complement(13640..14224)
                     /locus_tag="PLH85_12640"
     CDS             complement(13640..14224)
                     /locus_tag="PLH85_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNB65460.1"
                     /translation="MSLSCPTADQLRRRFAESPAIETPVEEGVPEELRPAAVLVPLVM
                     RDTPTVLLTRRTDHLHHHPGQVSFPGGRMEEHDASPIETALRETEEEIGLQRQHVELL
                     GHLPVYRTGTGFEVTPVVGLVAPPFDLKVDDFEVAEVFEVPLAFLLDSANHQRHRVVV
                     RGALREYYAMPYGNYYIWGATAGMLVSLQRFLSA"
     gene            complement(14242..14850)
                     /locus_tag="PLH85_12645"
     CDS             complement(14242..14850)
                     /locus_tag="PLH85_12645"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNB65461.1"
                     /translation="MAAMDLAQATDVVLAGVFPGKALLVIDGRAPRAVASGAMTPEGV
                     RVITVEGDTATVEVDGRRQRLVVGQSAVRVGGETSGGGTVELQADRAGHFLTYGTVNG
                     AQVNFLVDTGASLIVLGRSDAIRAGIDYKKGEPAQSITANGVARTWIVMLDSVRVGNV
                     TQRNVRASIHGEDLPVALLGMSFLNRMEMRRDNGVMTLKQRY"
     gene            complement(14990..15754)
                     /locus_tag="PLH85_12650"
     CDS             complement(14990..15754)
                     /locus_tag="PLH85_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNB65462.1"
                     /translation="MAGLAQRRRVLAGLGALATTWAMPTWADFPDVVAQIKNGVVAVG
                     TFLPSRNPQFKFRGTGFVVGNGNLVATNIHVVSDALEENETLTVALPGTGRVIGRAVV
                     RQASDADHDLVLLRFDGPPLPALRLGDSGAVRDGQEVAITGFPLGASLGLVPVTHRGI
                     VSAVTPVGMPAPSASQLDARTVRRLAMGAYNVFQLDLVSYPGNSGSPLFDTHSGEVVG
                     ILNMVFVKGTKESAVAMPTGISYAIPTHYLKALLVP"
     gene            complement(15757..16569)
                     /locus_tag="PLH85_12655"
     CDS             complement(15757..16569)
                     /locus_tag="PLH85_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB65463.1"
                     /translation="MLVEQAVAFEHGEGVPRDLERAAVLYCEAARAGDPEAMYSLGWM
                     YLNGRGVLRDDAVATTLFDMAIVKGHDGALRARRFASDYTGAVPDCLKDRPADHGFAA
                     RDSWDTQKYLASLPDQKKKVVEILTTLAPRFSIEPRLALAIASTESNFDTGARSPKNA
                     MGVMQLIPETASRFNVRDSFDPVQNIKGGLAYLRWLLAYYRGDVALAVAGYNAGEGAV
                     DRYRGVPPFKETQNYVRRILAFFPRTDHPFDARLVDASPIVQTFRAVTRFKD"
     gene            complement(16689..>16870)
                     /locus_tag="PLH85_12660"
     CDS             complement(16689..>16870)
                     /locus_tag="PLH85_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB65464.1"
                     /translation="WDKLPKAGAMVAAVAHKEYAEMGVGALAEKIVSGGVFTDVKSFY
                     DPAQLQAAGLSVWRL"
BASE COUNT         2835 a         5882 c         5660 g         2493 t
ORIGIN      
        1 cgcagacaag gcgtggctgt cgagagggcg ttccggcggg atgatggaaa ggaatacccg
       61 cgcggggaac gtcgtgccgc cgcagcagcc gggcacgccg cggcattggg gcgacacgct
      121 ccgtcggatt tgcgtcccgc gttggggggc cgaggggcgg cgcgatcagg gcgtccccgc
      181 cgtccgcggc gagcatgctg gctggtgtgc ggaggacgtg ggctagggct ggcgtatggg
      241 gatcggccgc catgcgggcg agggcgcccg tgccgccctc ctcccccgct gcagtgcggc
      301 gtgcgcttca gccgaactgg cgtccgaagc gtcccatgcc cagggccgcg acgctgaccc
      361 ccagggcggc catggacagg accgagggcc acagcaggtc cagcccggcg cctttgagga
      421 ggatgccgta ggcggcgtcg aggaagtagt gcagcgggga gatccacgcc aggaggtgca
      481 tccattcggg catggcctcg atgggggtcc atgcgccgga gagcaggagc atgggcatca
      541 ggatcaccac ggtgagcatg cccacctggg ccaggttgcg ggcgacggtg gcggccagca
      601 ggcccaggcc ggcggtggtg aagacgtaga gggcggtgat gaggaagaag ctgacgaggc
      661 tgccgcgcat gggcacgtcg aaacagggca cgaggatgcc gaacagggcg atggcggtgc
      721 cggcgaggat caccagggtc atggccacca ccttggggca caggatctgg aaggcgctca
      781 ggggggtgac catcagttgc tcgatggtgc cccgctcctt ctcccgcacc atggccgccg
      841 ccggcagcag gatggagaac agggtgatca cggtgagcag ttcggagatg cccatgaacc
      901 acgcgtcgtt ctgattgggg ttgtaccaga cccgcgtctc cgcggcgagg gtggggccgg
      961 ccacgccgcc gctgcccagg ccttcgcgca gggcggcggt ctccagggcg tacttgccga
     1021 cgatctgggc gccgtagctg gcggcgagga agccgagcac ggaattggaa gtgtccacct
     1081 gcagttgcac ggcggcctgg ccgccgcggg cgagggtttc ctcgaagcgc ggcggaatgt
     1141 cgaggagggc catcacgtcg gcgttgtcca ggtggcggat ggactgggcc ggcgtgccgg
     1201 ccgcaccggc cacgcggaaa tacggcggct ggaagcggct gctcagttcc cgcgaggcaa
     1261 aactggcgtc gccgtcgagc accgccagcg cggcgttctt gagctgcatg gagacgccgg
     1321 acgcggcgat gtagatgtcg ccggtaaagg catacacgaa gaagaccatc agggccacgt
     1381 cgcggaagag ctggatcagc tccttgcggg tcatcacccg caggcggcgc aggccggccg
     1441 cagagaagag tccgttcatg atttgggccg cttggtgaac agcaggtagc cgatgccgaa
     1501 caggcccagg gcgtaggcgg ccagcaccag cacgtcgccc cacagggcgc cccagcccac
     1561 gcccttgagg aagctgccca ccacgatgtt ggtgaagtac atgggcggca gggcatgggc
     1621 aatggccagc gcgctggggg ccagggacga caggggcagc agcatgccgg agtagagcac
     1681 cgcggggatg atgctgacga tgctggcgac gatcatggcc gccacctggg tctgcaccag
     1741 catggaaatg accagcccga tgccggtggt gcacaccacg tagagcagca gcgccagcgc
     1801 gaagaacagg ggatcgccct tgaagggggt gccgtacagc cagatcgcca gggcccacag
     1861 gatgagcccg ttgatgaagg agatgagcac gtagggcgcc agcttgccga tcaggaattc
     1921 ccccggcgac acggtggacg cgtagatgtt gaagatggag ccggattcct tctcgcgcac
     1981 cacgcccagg gcggtgagga agggcggcga cagcatcatg atgagcatga tcagcttggg
     2041 cgccagggac cagatgctct tcacgctctg gttgtagagg tagcgcacct ccagcttcac
     2101 cggctgcacg cgggtctggg cctcggccag gggaatgccc cgcagggccg acagggcctg
     2161 ggccatgctg tccaggctca ccgcgctatg gatggcagcc acatagccct gggtggtctg
     2221 ggcgcgcatc gggaaggtgc cgtcgatgag cacctggacc ggcgctggct cgccccgtgc
     2281 cagatgttcg ccaaagcgcg ggggaatcac cagggccagg cggatgctgt tgtcggcgag
     2341 caggcggtcg agctggcgca cgtcctccac ctggcccttg aagtcgaaat agcgggacga
     2401 gacgaagcgg taggcgtagt cccggctcgc cgcgctgcgg tcctggtcca ccacggcgaa
     2461 gggcaggttc tccacgtcca gggtgaggcc gaagccgaac agcaccatga gcagggtcgg
     2521 cacgaagaag gccagggcga agaacagccg gtcgcggacg atctcgcgcc attccttcca
     2581 ggccagggag acgatgcggc ggccgttcat ggcgccctcc gtgcggcctc ttccagcgcg
     2641 gtgatgcgct gcacgaagac gtcctccatg gacaagggac gcaggccggc cgcctgcacc
     2701 tgcacgcccg cggcggccag cacgcccggc agacgcgcca gatcgccgtc cacatcccgc
     2761 gacagcaggt gcacggcgcg cccgtgcagg gccgcgctgc catagcccgc cgccttcaag
     2821 gccgcctggg ccggcaccgg ccgatccacg cgcagctcca gcaggcggcc ggactcttcg
     2881 gcgatggcgg ccttcatggc ttcgggtgag gcgtccgcca ccacgcggcc ggcgtacatc
     2941 agggccaggt ggtcgcagtg ttccgcctcg ctcatgtagt gggtggtcac caggatggcc
     3001 acgccctcgc gccgcgacag gtggaagagg atgtcccaga acatgcgccg gccgatggga
     3061 tccacgcccg atgtgggctc gtcgagaaag agcacccgcg gctcatggac cagggcgcag
     3121 cccaaggcga ggcgctggcg cagccccatg ggcaggcgcc cggccccgtc gtgctcgaag
     3181 ccggccagcc cggccatgcc gatcacccag tcggtgcggc gctgcgcctg gcggtcgtcg
     3241 agcccgtaga tgccggcgta gaggcggatg ttctccacca ccgagaggtc gtgatacagg
     3301 gaaaaggcct ggctcatgta gccgatgcgt tccttgatgg cgaggcccgc cgtgcgcatg
     3361 tcggcgcccg ccacccggcc cgcgccggac gagggtttga ggatgccggt gagcatcttg
     3421 atgaccgtgg tcttgcccgc gccgttggcc cccagcaggc caaagatctc gccctggggc
     3481 acggcaaagc tcaccgcgtc ggcggcacgg aattcgccga agacacgggt cagctcacgc
     3541 gcttcgatgg ccagttcgcc cggcgcggcg gcgcggcgcg tggccccttc gacgcggctg
     3601 tcgagctgcg cgccgcggcg gcgcagcagg gcgacgaaga cgtcctccag ttcgggctcg
     3661 cgggcgaacc agtcgcggag gtcgatggtc ggcgcagcgg tgcccgtcgt cgtcgcggcc
     3721 ggcgcgatgt ccaggcaggc cgccacggcg ccgcgggccg cgtccgcgtc cagcccctcg
     3781 gcgaacacgc gaaaccaggc gcccatcgcc tccgtctggg ggaagcgggc cttgagccgc
     3841 ggcagggcct cggcctgggg ttcggcgcgg aacagggcca gggtgccggg caccgtggcc
     3901 accagcgcag cgggcgtgcc ttcgcccatc accttgcctt cgtggaacag ggacacccgg
     3961 tggaagcggt ccgcctcgtc caggtaggcc gtggacacca gcgcggtgat gcccttctcc
     4021 tccagcaggc gcccgaggat cgcccagaag tcgcgccgcg acaccgggtc cacgccggtg
     4081 gtgggttcgt cgaggatgac caggtccggc tcgtggatca gggtgcacac cagcccgagc
     4141 ttctgcttca tgccgccgga gagctggcgc atggggcggg cgcgaaaggc gtccaggcgg
     4201 gtcatcgcca gcagcagcgt cttgcgctcg gccagttggg cggcgctcac gccgcgcagg
     4261 cggccgaaga aatccacgtt ctcctcgatg gacaggtcgg gatacaggtt gagcccgagc
     4321 ccctggggca gaaagccgat gcgctgcttg acccgctccg ccgcccgctc ggaatccacc
     4381 ggcaggccga agacctccac gcggccgcct tcgtaggcga gcacgccggc cacggccttc
     4441 atcaggctgg atttaccggc gccgtccgcg cccaccagcc cgtagatctc gccgggctgc
     4501 acggtgagcg tgagcccgtc ggcggccacg cgccggccgt agcgcttgac gaagccttcg
     4561 acgcggatcg ccggggcgtt catgaccgac tcaccagcgc ggcgcctgcc agggcacctc
     4621 gtccttccag cggatcaccg catcggccgg cacgcccggc gccaggcgat gctccggatt
     4681 ggcgtccagg tagagcttga ccgcgtaggt gagcttgacc cgctcgtccg gcgtctgcac
     4741 ttccttgggc gtgaactcgg cgcgggaggc gatgtagcgc gtcgtggcgg caaaggcctg
     4801 agcagggaaa gcgtcggaat acactcgcgc cggcagcccc aggcgaagct ggccgatgag
     4861 cttctcgggc acgtacacct tgagatagag ccggtccaga tcgacgatct cgaacaccgg
     4921 cgtgcccgcc gccaccatct cgccggattc ccgcaggcgg ttgaggacca cgccgcccac
     4981 cggcgcccgc agggacaggt cgtcgatcag gctggccacc tccttcgccg ccgcctgggc
     5041 ctgatgcaga ccggcttcca gcgcagccac gtcgttctcc cgcgccttga ccttgtcgtg
     5101 gcccagatgg gccgacgcca gggcctgctc ggcctggcgc agatcggccg cggcgctgct
     5161 gcgcaccagg cgcatctgct cggcgcggcg aacctccacg gtgccgcgct cggccagttc
     5221 gtcgaagcgg gcggcgtcct tggcggcctg ggcgtcggcg gcccgcgcct tggcgagctg
     5281 ggcgtcggcg ctggcctcgg cgaggggcac ttcgcgccgc gccacggcca actgggcctg
     5341 agccgccgcc acttgcgcct ggagcgtggc caccgcggcg tccgcctgct gctgacgggc
     5401 ggcgagctgg gtgtcttcga ggcgggccag cacctgcccg gcctgcacgc tgtcgccttc
     5461 gcgcaccttg agcgccgcga tgcgcccggc cagcttgctc gccaccgtgg tgcgatcgcc
     5521 ctcgatgcgc ccgttggcct ggatcaggcc ctcgggcagg gccgcgctgc cggcccattc
     5581 gtgccaggcc cacccgccgg ccgccagcac ggccaccacg gctacggcac cgccgatgcg
     5641 gcgtgtcagg atgttcatgg aagggattcc ttagtcaaaa gaccggcccg catcagcgcc
     5701 ccccaaggga gccggcgggg cgccgttcag agttctccga ccgccttctt caggcggtag
     5761 cgggccccat gcaggtcgta atgggcgttg aggtgattgc tgtcggtgag ggcgcgcaag
     5821 gcctcggcgt ccagcacttc ggtgtgggtg ccgacgccgt tgcgatagcg gtcgcgggcc
     5881 acgcgcaggt tctcgtcggc ctgggcctcg gcggcgcccg tggtggcgag gcgctgggcg
     5941 gcctcttcca cggccagcca ttcgcgccgg acttccagct cgatcagcgt ggcggtgtcg
     6001 gcctgacgtt cccgcgcggc acgggcccgc gccgcggccg catcggcgcg ctgacgctgg
     6061 atgccgccgt cgaacaattc ccactgcacg cccacggcca cctgggcgat gtcctgactc
     6121 gcccggtagc ggttgtcctc gtgcatcacc gccgcattga cgcccacctg gggccggcgg
     6181 gcggcgcgtt ccatgtcggc ctcgcgatcc gtggcctcgg cgcgggcgcc cagcagggcc
     6241 agctccggcc gggcggcgcg ggcgcgggcg acgagggtgg aaaagtcttc gcgggccgcg
     6301 tcgggcggcg gcggttcgtg cacgtccacc gccgtctcca gcggccggcc gaggagccgg
     6361 ttgtaggccg ccgcggcgag atccacgccg tgccgggcgc gcgtctgctg ctgacgggca
     6421 tcggccagcg cgacggccac cgacagcacg tcgttgcgcg gcaccacgcc ctggcgatgg
     6481 agggcattca cgtccgccgc gtgggcgtcg agggccgcca cgtgacgctc cgccgccgcc
     6541 agcgcgcgcc ggctgcgcag cacgttgagc caggcctcgg ccacgtccag cttgaccgtc
     6601 tgctcggtgc gcaggcgttc ctgggcgctg gcctgcacgc cgatgtcggc cgctgccact
     6661 gccgccgcga tgcgcccgcc cgtatagatc ggcaggctcg cctgcagggc atgggtggcg
     6721 taggtgcgct cggacagggg catgcggtcc ggcaggctga tgccggggat cgtcggcagg
     6781 ccgatgaggg ccgcgggctc cttgctcagc acggtgtagc gggaactcgc cgtggccttg
     6841 ggcagggcca ggcccttcgc ggcctccagc gtggcgcggg cgccctcgcc ttcggcatcg
     6901 gcggcgcgca gacgcgcatc taccgacaag gcctgggccc acgcgtcggc gagggtctcg
     6961 gcggcccaac ctgtcggcgc cgcggacagg agcaggaccg acagggcgat ggaaagcgga
     7021 cgaaatcgca tcgggagggc cgcccggccg cgtgcttgac cagcggatga cgtcgcgtcg
     7081 aagtgcctct tcatcacgac ctccaattac tgactcatcg gtcactaaac tactccgccc
     7141 cgcacgcggc agccttgcgg aaagtcaaca gagcgcacgg cctcgcccgc gacgacgccc
     7201 gtgtcggaag cattccgccc atttcccgga acggggacga tgcagcacat cgcgggcttc
     7261 gcacagcgca aagaggctgc acacgctgca tcgcccgcct ccctgacatc gtcacccagc
     7321 cgccaggacc tggccccgga tttgcatagg cctgctgatc acgcccactc ccaggaacgc
     7381 tccatgcccc ttcccgccct cgacgacgac ctcgtggact tcggtgacat ccccgcgccc
     7441 atcaacgccc tcctccagga aggcgtgtat tggcaccccg ccgatccggc ccgcgcccgc
     7501 gcctgtttcc gcagcgccat cgacctggcg ccggacgtgc tgccgtccta ccgctgcctc
     7561 ttcaaggccc acaacaagca gcgggacttc gccgccgcct acgccgtggc cctggaaggc
     7621 ctgggccgcg cggccatgca ggccggcatg agcccggact ggagcacctg gacgcgcccc
     7681 gccctggccg ccgcgccgac ggtgccggcg cgctttctgc tgcattccct caaggcggtg
     7741 gccttcattg aactgcgtcg gggccacgaa tcggcctccc gcgcagccct cgcccatctc
     7801 caggcgctgg acccggaaga cggcgtcggc gcctcggtga tcgccgccct cctcgaccgg
     7861 gaagacgcct gatgcgtcgc gaatccgaca cgccgccctt tgcggtgacg caaaccgtac
     7921 atcgcgccct cggcccgctc gtggtgctgg aggtgggcgc gctgaaggtc cgcctcacgc
     7981 cgcgggaggc ccataccctg gcgctggccc tcttcgccgt gcgggatggg cgcagcgccg
     8041 agaccgagct gttcatgagc cccatcgcca gcgacggcat cttcgtcgcg ccggtggggt
     8101 cggacggcat cgccgtcgag acgtccgccg gaccggtggc gctggactgg caggcggtgg
     8161 gcgagctggg tgcccagctg tcgcccgcgg cagtgccggg ctgaggcgct ccggctacaa
     8221 gacgaccgca gcggcggcgg cgttttgccc ttggccgaac ccatgcagcg ggcgtggcca
     8281 gcgcccggcg cccgggtttg cgcggttcgc ccccaaggat catcgggaat gccggacggc
     8341 tgtggtattt ttgcggcctt taccgcccac cgggccgcct gaacaccccg tcaccgcgcc
     8401 atgaacgaca cgccggatct cttcgacacc ctgcctcccg acacgcccga accgccaccg
     8461 ccgcccgtgg ccacgccgct ggccggcagt gacgacgccc tgcccctcga cctctacgcc
     8521 gagcgcgcct acctctccta cgccatgagc gtggtgaagt cccgcgccct gccccaggtg
     8581 gaggacggcc tcaagccagt gcagcggcgc attctcttcg cgatgaacga gatgcgcctg
     8641 tcgcccgcgt ccaagcacgt caagtcggcg cgcgtcgtgg gcgacgtgat cggcaagtac
     8701 cacccccacg gcgactcgag cgtgtacgac gccatggtcc gcgtcgccca ggatttctcc
     8761 ctgcgctacc cgctggtgga tggccagggc aacttcggct cccgcgacgg cgactccgcg
     8821 gcggccatgc gctacaccga gtgccgcctc acccccatcg ccgagctgct gctgtcggag
     8881 atcgaccggg gcaccgtgga tttccgtccc aactacgacg gcgccttcca ggaaccccag
     8941 ctcctgccgg cgcgcctgcc cttcgtgctg ctcaacggcg cctccggcat cgccgtgggc
     9001 atggccaccg agatcccgtc ccacaacctg cgggaagtgg cccgcgccgc gattcacgcc
     9061 atcgagcacc cggaggccac cgtcgccgac ctgctcgggc acatcgccgg gccggacttc
     9121 cccggcggcg gacagctcat ctcgccggcg gcggacatcc gccaggccta cgagaccggc
     9181 cgcggcagcc tcaagctgcg cgcccgctgg atcgtcgagg acctcgcccg cggtcagtgg
     9241 cagctggtga tcaccgagct gccccacggc gtgtcggccc agaaaatcct cttcgagatc
     9301 gagacgctga ccaatcccca gcccaagacc ggcaagaagt ccatcgaccc ggatcaggcc
     9361 cagctcaagg ccctgattct gtcggccctc gaccgggtgc gcgacgaatc gggcaaggac
     9421 gcgccggtgc gcctggtctt cgagcccaag agccgcaatc aggacgtggc ggagttcgcc
     9481 aacctgctcc tcgcccacac cagcctggaa acctcggtcg ccctcaatct ggtgatgatc
     9541 ggcctggacg ggcggccggc ccagaagaat ctggccgaca tcctgcacga gtggggccgc
     9601 ttccgcaccg tgacggtccg ccgccgcagt gtccatcgcc tgggccaggt ggatgaccgc
     9661 atccacatcc tggaaggccg ccacatcgtc ttcctcaaca tcgacgaggt gatccgcctg
     9721 atccgcgagt cggacgatcc caaggccgcc ctgatcgccc gcttcgacct gagcgaccgc
     9781 caggcggagg acatcctgga gatccgcctg cgccagctgg ccaagctgga aggcatcaag
     9841 atcgaacggg aactggccga gctgcgcggc gaaaagaccg agctggaagc cctcctcgcc
     9901 gacgagggca agctgcgccg gctggtgatc aaggaaatcc gggccgacgc cgagaaatac
     9961 ggcgacgcgc gccgcaccct ggtggaggaa gccgcccgtg ccggcgtcac ccagaccgtg
    10021 gtggacgaac cggttaccgt catcgtctcg gaaaaaggct gggtgcgcgc ccgcagcggt
    10081 cacggcctgg atctggccaa tgtggccttc aaggatggcg accgccttgc caccgcgctg
    10141 gaatgccgca cggtggatgc gctgatcgcc ctcacggacg gcggccgcgc cctgacggtg
    10201 gcggtgtcga cgctgccgga cgggcgcggg atgggcgccc cgctggcgtc atttgtcgag
    10261 ttgccggcgg gggcgaagat cgttcacgtg ctgagcggcg atccggccag ccgggtgctc
    10321 atggcgaccc gcgacggcta cggtttcctg tgccgcttgg gcgacatgct ggccaccaag
    10381 aaggccggca agcagttcgt cacgctggac gagggcgtgg gactattgcc accgttgatc
    10441 tacgccgacg cgccggacct gcacgtggcc accctgtcgg agaagggtcg cctgctggtc
    10501 ttcacgctgg accaggtgaa ggagctctcg ggcggcggcc gcggcgtgat cctccaggcc
    10561 ctggaagacg gcgacaccct gcgcgcggtg tgcgtggccg gcagcgacgg catcgtgctg
    10621 tccagcgcct ccagcgccgg gcgggcctac gaggtgccca tccccgcccg cgactggacg
    10681 ccctggctca acaagcgcgc ccgcaagggc aagcccgcgc cgggacgctt cacgcccacg
    10741 gcggtgacgc ccctcaagcg ggactgagga ccttcgccgc ccagaacagg gcggtaatgg
    10801 tcttggcgtc ggtgatgcgg ccgtccagca cggccgcttc ggcctcgtcc agggacatcg
    10861 ggaagatctc cagatattcc ccatcatcca gcgacgcgcc ttcgtggcgc agcccgctgg
    10921 ccagaaagat ctcgatgcgc tcgtcggaat agccgatgca cggatgcatc acgcccagat
    10981 ggcgccagga gtcggccacg tagccggtct cttccagcag ctcacgccgg gcgcagcgtt
    11041 cgatgtcctc cccggcgtcg atcttgcccg ccggaagttc gatgaacacc cgcccgcagg
    11101 gatagcggaa ctggcgctcg aagatcagat ggccgtcggg caactgggcg atcaccacca
    11161 ctgcgccggg atggcggatg tattcgcgga caccctgctt gccgtcgggc aggcgcaccg
    11221 tgtcccggtg aacgttgagg agaacgccgt cgaacacctg ctcggacctc acgcaggttt
    11281 cggtcaactc tgctgccatc acacactcca tgaaaaaagc ccggcaggac agagccgccg
    11341 ggcttcgggg tccacacgca ttcgcgcctg ctcccgggga agccggcgcg aaccgtcaga
    11401 cctgcatcag atgcgctcga acacggtggc gataccctga ccgccgccga tgcacatggt
    11461 caccagcgcg taacggccgc cggtgcgttg cagctcgtac agcgccttgg tggcgatgaa
    11521 ggcgccggaa cagccgaccg ggtgaccgag ggcgatggcg ccgccgttcg ggttggtctt
    11581 gaccggatcc agacccagcc cgcgcatgac ggtcagggcc tgggcggcga aggcttcgtt
    11641 ggactcgatg acgtccatct ggtccagggt cagaccggcc ttcttgaggg ccagcttgga
    11701 ggccgggatc ggaccttcac ccatcacgtc gttgggtaca ccggcgatgg cgtaggaaac
    11761 caagcgggcc attggcttgt agccggcgtt caccgcggtg gcgccatcgg ccagcacgaa
    11821 gaaggcggcg ccgtcgttga tgccggaggc gttgccggcg gtgacggtac cgtccttctt
    11881 gaacgccggc ttcatcttgg ccagggattc cagcgtggtg ccgcgcttga cgtgttcgtc
    11941 ggtgtcgaac accacgtcgc ccttgcgggt ctgcttcacg atgggaacga tctgggactt
    12001 gaagcggcct tcgtcgatgg ccacagcagc acggcgctgg gattcgacgg cgaaggcgtc
    12061 ctgctcttca cggctgatgc cgtgcttgac agccaggttt tcagcggtga tacccatgtg
    12121 gccgacaccg aacgggtcag tcagcacggc caccatgctg tcgatggcct tggtgtcacc
    12181 catgcgcgca ccggagcgca gcgccggcag catgtaggtg gccttggaca tgacttcgac
    12241 gccgccaccg ataccgtaat cggcatcgcc cagcaggatg ttctgggcgg tggtgacgat
    12301 gccctgcaga ccggaggagc acaggcggct cacctgcatg gccacggact ccatcggcag
    12361 gccggcctgg atggacgcca cgcgggacac gtaggcgtaa cgggcgtcgg taggaatgca
    12421 gttgccgacg gtgacatagt tgatcaactg gggatccacg ccggaacgaa ccaccgattc
    12481 cttcatcacg ataccggcca gctcgctggc gtcgaaatcg gcaagggaac caccaaacgc
    12541 gccgattgcg gaacgaacgg cgctcagaac cactacatca cgactagcca tctagtaacc
    12601 ctcctcttat gagaccatca atgaccaacc caacccgcca cacccaagag cgccagtacc
    12661 agaaatacca gcaccagggt gcgccagatc aggccgatcg tgctttgcat gaaatcggca
    12721 tcagcctcgt cgccaatgcc catctcggga cgctcgacaa tttcgcccga ctcgtggacg
    12781 ggcatgccga gccgtacgcc cagcgcccca cccccactcg cgagcagtat accggaagcc
    12841 ttgtctgccc attgggcagc ctgggctctc cagcaataca ccgcatcttc gaagtctccg
    12901 acgatggaaa acgccgccgc ggacaaacgc accggcaacc agtccagggc cgcgaacgcg
    12961 cgatgggcga attcaccgaa atcgccgaac tccggctcac ccttgcgcag actccattcc
    13021 tcgtcgaaga aacgggccag ccggtagagc aatgcgccgc tcggcccggg cagcaggata
    13081 aaccagaaga tcaccgcaaa cacattgcgg tgtgccgcaa tcagcgcctc ctcgatcgcc
    13141 agcctggcca cctcggagga gctcgatgca tcatgactgc gcttgcgcca gacgccgatc
    13201 agttgccggg cgcgcgccag ctcacccatc cgcagggcca gttgaagatc ggaaaagaaa
    13261 cggctcaggt tgcggaacgc catggtgaaa tagaggatca ccacgttgaa gaccatcgcc
    13321 agcaacggat ggacgtgcca caacacaaaa taggccagcg ccgacacgaa tcccacgctc
    13381 agcatggcca cccaccaggc gaccgccccg tgacgcgcct cgccgtcgtt gaagcggtct
    13441 tcgaggaagt gggcgaacgc cttgagaggc gcctccacga accgttcggt ggaaatgggc
    13501 cggagatgct cgatgagcag ggcaacgatc agggaaaaca gggtcatgcg cagtggggcc
    13561 ttggtcagcc ctcgacaaac tggcctattg gggaagcgct aagataccac cattccacga
    13621 accccggaac ggcggccgat caggcactga gaaagcgctg caggctcacc agcatgcccg
    13681 ccgtggcacc ccagatgtaa tagttgccgt agggcatcgc gtaatactcc cgcagcgctc
    13741 cacgcaccac gacccgatgc cgctgatggt tggcggaatc gagcaggaac gccaggggta
    13801 cctcgaacac ctctgccact tcgaaatcat ccaccttcag gtcgaagggc ggcgcaacca
    13861 gccccaccac cggtgtgact tcaaaacccg tccccgtgcg gtacaccggc agatgcccga
    13921 gcaattccac gtgctgacgc tgcagaccga tctcctcctc cgtttcccgc agggcggtct
    13981 cgatcggtga ggcatcatgt tcctccatcc gtcctcccgg aaagctcacc tgtccgggat
    14041 ggtggtggag atgatccgtg cgacgcgtca gaagcaccgt cggcgtgtcg cgcatgacga
    14101 gcggtacgag gacggcggcc gggcgcaatt cctccggcac gccctcttcc acgggcgtct
    14161 cgatcgcggg agattcggca aacctgcggc gcaactgatc cgctgtggga cacgatagac
    14221 tcatgaggtc acgcacgacg ctcagtaccg ctgcttcagg gtcatgacgc cgttgtcgcg
    14281 ccgcatttcc atccggttga ggaagctcat gcccagaagc gcgacaggaa gatcctcacc
    14341 atggatgctg gcccgcacgt ttctttgagt cacgttgccg acacgcacgg aatccagcat
    14401 cacaatccag gttcttgcca cgccgtttgc cgtaatggac tgggccggtt cgcccttctt
    14461 gtagtcgatg ccggcccgta tggcatcgct acgtcccagc acgatcagac tcgcaccggt
    14521 atcgaccaga aaattcacct gagccccatt caccgtaccg taagtaagga agtgaccggc
    14581 acgatcggcc tgaagttcca ccgttccgcc gccggatgtc tctcccccca cacgcacggc
    14641 cgactgcccc accaccagcc gctggcgacg cccgtccacc tccacggtcg cggtatcacc
    14701 ttccaccgtg atgacacgca cgccttccgg cgtcatggcg cccgatgcaa ccgcccgcgg
    14761 cgcacggcca tcgatcacca gcagcgcctt tcccggaaag acgcccgcca gcacgacatc
    14821 cgtcgcctgc gccaggtcca tcgccgccat ggccaaggca gccaggacgc cggacgccca
    14881 cctctgtgat attgaaaacg cttgaaagcc cgccacgtcc cgccacccgt ataccgacac
    14941 ccgtcattct gccatggggc gcgcaccatg cgccctcggg ggacccgcgt cagggcacca
    15001 gaagggcttt cagatagtga gtcgggatgg cataggagat gcccgttggc attgccacgg
    15061 cactttcctt ggtgcccttg acgaacacca tattcaggat gcccacaacc tccccggagt
    15121 gggtatcgaa gagcggactg ccactgttac cgggatagga caccagatcg agctgaaaca
    15181 cattgtaggc ccccatcgcc agacgcctga ccgtacgggc atcgagctgg ctcgccgacg
    15241 gtgccggcat gccgacgggt gtcaccgcgg agacgatgcc gcgatgggtg accggcacga
    15301 gccccaggga agcgcccagc gggaacccgg tgatggccac ctcctgaccg tcccggaccg
    15361 cgcccgaatc gcccagcctc agcgccggca gcggcggccc gtcaaagcgc agcagcacga
    15421 gatcgtgatc ggcatcggac gcctgacgca ccactgcacg cccgatcaca cgccccgtgc
    15481 cgggcagggc caccgtcaag gtttcgtttt cttccagggc gtcggacacc acgtggatat
    15541 tggtggcgac gagattgcca ttacccacga caaatccggt tcccctgaac ttgaactggg
    15601 gattacggga cgggaggaat gtgccaaccg ccaccacgcc gttcttgatc tgggcaacga
    15661 catccggaaa gtcggcccat gtgggcatcg cccatgttgt ggccaatgca cccaagccgg
    15721 ccaacacccg tctgcgctgc gccagtccgg ccatgttcaa tccttgaatc gggtcacggc
    15781 ccggaaggtc tgcacgatag gcgacgcgtc caccagccgc gcgtcgaagg gatgatcggt
    15841 gcggggaaag aacgccagga tacgccggac gtagttctgc gtttccttga acggcggcac
    15901 gccgcgatag cgatcaacag ccccttcgcc cgcattgtag ccggcgaccg ccaaggccac
    15961 atcgccccgg taataagcca gcagccagcg caggtaggcg aggccgccct tgatattctg
    16021 taccgggtcg aaactgtcac ggacgttgaa gcggctcgcc gtttccggaa tgagctgcat
    16081 cacgcccatc gcattcttgg gcgagcgtgc gccggtgtcg aaattggact cggtgctggc
    16141 gatcgccaac gccagccgcg gctcgatgga gaagcgaggc gccagggtgg tgagaatctc
    16201 caccaccttt ttcttctggt caggaagcga ggcaaggtat ttctgggtat cccagctgtc
    16261 ccgagcggcg aaaccgtggt cggccgggcg atccttgagg cagtccggca ccgcgccagt
    16321 gtagtcgctg gcaaagcgcc gcgccctgag cgctccgtca tggcccttga cgatggccat
    16381 gtcgaagagg gtcgtggcga ccgcgtcgtc ccgcaggacg ccgcgcccgt tgagatacat
    16441 ccaacccagg ctgtacattg cttccgggtc gccggcccgc gccgcctcgc agtacagcac
    16501 ggcagcccgt tcgagatcgc ggggaacacc ctccccgtgt tcgaaggcca cagcctgctc
    16561 gacaagcatc cgggccccgt cgacacctga agggagcacc gcgtccccgt ccgccacggc
    16621 agcctgcccg acgctcgcac ccgtcaggca gatcaccgca ccgaccgccc gcaggcagcg
    16681 gaggagactt acagccgcca cacgctcagc ccggccgcct gcagctgggc cggatcgtag
    16741 aagctcttca cgtcggtgaa cacgccgccg gacacgatct tctcggccag cgcgcccaca
    16801 cccatctcgg catactcctt gtgggccacc gcggccacca tggcgccggc cttgggcagc
    16861 ttgtcccagg
//
LOCUS       DAOQMM010000082        16637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1324_length_16637_cov_35.186105, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000082 DAOQMM010000000
VERSION     DAOQMM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16637
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_16637_cov_35.186105"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            497..2161
                     /locus_tag="PLH85_12665"
     CDS             497..2161
                     /locus_tag="PLH85_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65465.1"
                     /translation="MKALTDLPVGVRLTIVIVVALLGTILLTALSLVQFHAVEDKAQA
                     LASRVNTVRLAQRITVRALDARSHLALALQHDPERPDIVQLHSHQVGAHLEAVARDLS
                     DIRDAMEALHTPDSGLSAEEAAALAGLEPEVARFESQGVGPMRERIEAGAFHDANVIL
                     LKLTNPAYEAVKSQASTLIDRIAARTDAGRAEMDAEVKRTTMLVIFGGVALGVLCLIV
                     GLRVTRSVTRQIGGEPKEAVRLMQQVAEGDLSMGAVTAPAGSMLASLGAMVVSLRGIV
                     SNIDHAASRVAAGSECIGNASRAVADAAGVQSDATASMAAAMEQMTVSISHISDSARE
                     TATESAHSDHLAEDGAVRVASARQEISRIAVTVTEVSSAIRRLAERADQISSIAGVIK
                     EIAGQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTAAATVEIEGTITTIQTD
                     TVRVVKVMDSAIPQVGSGVEAAEGAAQALQAIREGAHVALRRIDEVADAMREHSQTST
                     SIAQRVEEIARMIEETSTAMRQTADTASELENVSATLRQTVGCFHC"
     gene            complement(2364..3443)
                     /gene="tal"
                     /locus_tag="PLH85_12670"
     CDS             complement(2364..3443)
                     /gene="tal"
                     /locus_tag="PLH85_12670"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNB65466.1"
                     /translation="MNPIQAARAAGQQIWLDNLSRALINEGTLARHIADGVAGVTTNP
                     AIFHKAIAEGLDYLTDLDALRARPLDAEARYEALVIQDVQRACDLIRPVFDASQGDAG
                     YVSLEVSPALADDAAGTVAAARRLAAAVARDNLLVKIPATAAGVHAIETLTGEGLSIN
                     VTLMFSPAHVEAVAAAYVRGLEALAAKGGDLSKVRSVASVFLSRWDSLVDKQLEEIGS
                     EAALALRGRTGVAFARRAYQQYLARFHGDAFAALAARGARPQSMLWASTGTKNAAYSD
                     LLYVEALIGPETVNTLPDATLAAFLDHGRVTPGAVTAGVADADAHIAALASFGLDLDV
                     LGAKLQHDGLAQFATAFAKMLELTA"
     gene            complement(3492..4247)
                     /gene="gspN"
                     /locus_tag="PLH85_12675"
     CDS             complement(3492..4247)
                     /gene="gspN"
                     /locus_tag="PLH85_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNB65467.1"
                     /translation="MRKLALFLLIFLVLALMRAPATLLDAAFSHTTGAGLRLLLADGS
                     LWAGRGTLASSDPGGRSVTPWLPLAWQFEPTALLQGALRWRFTSDARPVATVDLGPGG
                     ASVSELDLHTPANALLASIPHPIARAGWQGDLDLGASHWACAADGRCSGEARLLWRGA
                     RSALFPGRRFGDYEVRIQADRGQLQFTVATLEGEVRLDGRGSAAPGQPPRFDGQISGD
                     ADFLSRLPAIAGGAATPTPQAGRYTLHWPPGSR"
     gene            complement(4253..4762)
                     /gene="gspM"
                     /locus_tag="PLH85_12680"
     CDS             complement(4253..4762)
                     /gene="gspM"
                     /locus_tag="PLH85_12680"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNB65468.1"
                     /translation="MNAPIAAARVTPLARLLTAWNACSQRERRLILIAGSVVALGLVF
                     GLADWSAQERRRLDRQVPRAQAQLEQTQEAATEIAALRGRPPVQPLAGPALADAVGAA
                     AKARGLAVTVNSTGDGVQIRGNGDFDALVGWLGTLHSDQGLRVLRLEIQRAGSQASVD
                     ALLAGPAAP"
     gene            complement(4759..5979)
                     /gene="gspL"
                     /locus_tag="PLH85_12685"
     CDS             complement(4759..5979)
                     /gene="gspL"
                     /locus_tag="PLH85_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNB65469.1"
                     /translation="MTTRLLLALDEHWPADPACPWVLLGPDGQPTAEGHSEPRHWPAA
                     AEVEVVLTGAQCLWLEVPLPGAARRDLPRLIAYALEDRLLKDPDTQHLTVSHRRPAGD
                     GERDLVGVLVIARERLRSLLAQLAALGRSPRRVVAAVQTAPAGPEGWHLAVAGPDAVL
                     RSAVTTGVALDADLLIPLLAQQAATARAANQAPDALHVHLAPGSGGPDTAALARETGL
                     PVSMAEPYRWWSGADRHAASLLHGEFASREQGAAWLARLRPAALLAAAALGVWLLANV
                     GEVLWLRHSLDGVQTRIARVYATTFPNTPVVAPAAQMRQQLNQERARHSLLRDDDALA
                     LLAQAADALGTDALDGVAALRYEDGRLDLSLQGPAAARAPALGGLLAARGLLADVRRD
                     GNATHLVLRRESLQ"
     gene            complement(5976..6941)
                     /gene="gspK"
                     /locus_tag="PLH85_12690"
     CDS             complement(5976..6941)
                     /gene="gspK"
                     /locus_tag="PLH85_12690"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNB65470.1"
                     /translation="MSRRPVAPAVAPSSRPVQRGAAVILALLTVALVAGLAAAAVGDL
                     GVALDSVAGRHDQAQARQLARGAVDWARNVLAEDARSTSVDHLGESWAVRVPPTPVEE
                     GEVSGELQDLSGRLNLNNLIRADGASVEDVAAFRRLLGLVGVGDADAIALSSALLDWL
                     DADDLPRLPGGAESAWYGSQDPPRRAANARLAAVAELKQIRGFTPDLVARLTPFVAAL
                     PPDTTVNVNTAPAEVIAAVVPGLGLDGARQVVAQRARAWFKDVADFTARLPAGNASPD
                     TSRIATTSRHFLATGRARFGVSTVRMEVLLDRSQKWPDIVWQKLL"
     gene            complement(6938..7507)
                     /gene="gspJ"
                     /locus_tag="PLH85_12695"
     CDS             complement(6938..7507)
                     /gene="gspJ"
                     /locus_tag="PLH85_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HNB65471.1"
                     /translation="MKRATARGLTLIELMVALAIFAVLGVLSYRALAEVSTSRARLEE
                     NFQRWRNISRAVQRIDADLLQIAAPGGQNGVARSPALTVLRSAGGGSELQFLRLDDTR
                     GIRRYGFRAEDNRLVWLRWSGRDPADAPAVEPLLDNVREVRWHFLKDNLRSDAWPPDA
                     NQPDVLPDAIIVEIDLPDAGTLTRWIALR"
     gene            complement(7504..7878)
                     /gene="gspI"
                     /locus_tag="PLH85_12700"
     CDS             complement(7504..7878)
                     /gene="gspI"
                     /locus_tag="PLH85_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HNB65472.1"
                     /translation="MKPAPRGFTLLETLVALTILAIALTAAFRALGVATQSSAELRDR
                     LLGDWIAENRLAELRATHVWPEPGSNEGQAVQAGRSYRWREEIRGTPNPLFRRADISV
                     YSEDSGEHAIARLSGYVVKPLR"
     gene            complement(7875..8444)
                     /locus_tag="PLH85_12705"
     CDS             complement(7875..8444)
                     /locus_tag="PLH85_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB65473.1"
                     /translation="MSAAHPFPIPTPGLPPARHARRADAFTPRPPRCAAGFTLIEVMV
                     VLVIMGLMATGISVGLDSLRGREADQALRRLRLVLEASADRAAVRGRPLAIEFLPDGY
                     RFAALDADDTWRPLIDPPVFTERTLPEGLSWSGLRLDGQDAPQPRLQFGTQAPEYELR
                     VATPRGIALLTGKANGDVLLTPPDAPPRS"
     gene            complement(8441..8869)
                     /gene="gspG"
                     /locus_tag="PLH85_12710"
     CDS             complement(8441..8869)
                     /gene="gspG"
                     /locus_tag="PLH85_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNB65474.1"
                     /translation="MSQGPRRARGFTLIEVMVVIVILGVLAALVVPKVMSRPDEARVV
                     AARQDISAIMQALKLYKLDNRRYPTAEQGLQALVVKPGSAPVPENWKSYLEKLPNDPW
                     GKPYQYVIPGLKGEVDVMSFGADGVQGGEGFDADIGSWSL"
     gene            complement(8880..10082)
                     /gene="gspF"
                     /locus_tag="PLH85_12715"
     CDS             complement(8880..10082)
                     /gene="gspF"
                     /locus_tag="PLH85_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNB65475.1"
                     /translation="MTAFRYRALDAAGKETAGVLEADTGKAARGLLRERGLFPLEVAS
                     VSQGGGARSARRRLKDADLTLLTRQWATLLASGLTVEQALAALIEQAETDATRQLLAG
                     VRSEILAGYSLRAALDRYPQAFSTLYRASIAAGEKSGELARVMNQLADYLERSNALRT
                     KTLQALLYPAIVAGVALMVIIGLMTYVVPQVVSVFQQSKQALPLLTRALIAASAVLRD
                     WGWLMVLALIGAFAAGRVALREPHLKRRWDAWLLRLPVLGRHLRTLDATRFASTLSIL
                     AGSGVPLLAALDAGRQVIARLPLADAVAVAAERVREGQPLSRALGASRQFPPLLIHML
                     ANGEATGRVDELLDRAARLQQAELENRTAALTALLEPVLLLLMGGFVLLIVMAVMQPI
                     IEINTLMK"
     gene            complement(10102..11565)
                     /gene="gspE"
                     /locus_tag="PLH85_12720"
     CDS             complement(10102..11565)
                     /gene="gspE"
                     /locus_tag="PLH85_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNB65476.1"
                     /translation="MSAPLLPYAYARSHGVFVASHGPEHADLILREGADLAAVAEVRR
                     SLGVPIRLESVGRAAFEARLAEAYSGADDNAAEVVADVGQEVDLTQLMTELPAVEDLL
                     ETQDDAPIIRMINALLTQAVRQAASDIHIEPYERHSVVRFRRDGVLVDVAQPHRALHA
                     AMASRIKIMASLDIAEKRLPQDGRIALRLAGRQVDVRVSTLPTTHGERIVLRLLDKGS
                     GQRGLESLGMAADTRGTFETLLREPHGILLVTGPTGSGKSTTLYAALQAMDARTLNIV
                     TVEDPVEYDLPGVGQLQVNSRIDLSFAKALRAILRQDPDVIMIGEIRDLETAQIAVQA
                     SLTGHLVLATLHTNDAASAVTRLVDMGVEPFLLASTLRGVLAQRLVRKLCPACRRDAP
                     ADAADRDALGAAPARLWHAPGCPSCARSGYAGRTGLYELMVADEALQRLIHTAADEAA
                     LRAHARAQGTRSLRDDGLRLLADGITSAEELLRVTRD"
     gene            complement(11562..13721)
                     /gene="gspD"
                     /locus_tag="PLH85_12725"
     CDS             complement(11562..13721)
                     /gene="gspD"
                     /locus_tag="PLH85_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNB65477.1"
                     /translation="MNPARRILAACLAAALALPLPPALAAKGDDADNRVSLNFVNADI
                     GAVIQAVSKISGRNFVVDPRVKGTLNIVTARPVERRNTYPILLSALRLQGYAAIEGDG
                     VTKIVPEADAKLHAVPVGKGQGSGGGDRLTTQVFTLKHESAAQIVPVLRPLISPNNTV
                     TAYPGNNTLVVTDYAENLGRIAQIIESIDVPQGDMRMLPLKHASALDLAATINRLLGD
                     GSAAGAAGQTGDTSQRVTVIGDARTNSLLIRSDNPSKIQAVRQLVGNLDQPGAAGNIH
                     VVYLKNADATKVAQTLRAVLSGESGTTGTSGSTATSSSFAPTGAGTFNSGTTGSTFGQ
                     NTATTTGGGNFSGSSGTSTGVPNATGGGVVQADPMNNALIITAPDTIYTNLRQVVDQL
                     DRRRAQVYVEALITEVSTDKAAEIGIQWQAGKVGTTSAYGGTAFTSSSSGSNLLTLAT
                     ALGSGTVTSPGNGLNLVLGSGSVTVGGKTIANLNMLARFLESDSRANILSTPNLVTLD
                     NEEAKIVVGRNLPFVTGSYATTGGTTTTNPFTTIERKDVGLTLKVKPQITEGGLVRMQ
                     IYQEASSVIDTTTANTYGPTTNKRSIESTVLVDDGATIALGGLVEDSFSGTIEKVPVL
                     GDIPYLGAFFRYDTRKRTKTNLIVFLRPVILRDAAAIRGISSDRYDYIIGQQRTMDSE
                     EQRFKFDRGLPSLPPAGSPPPATPTTVPQPSDRPAPQ"
     gene            complement(13718..14386)
                     /locus_tag="PLH85_12730"
     CDS             complement(13718..14386)
                     /locus_tag="PLH85_12730"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNB65478.1"
                     /translation="MNLPERLDLSRLTRLPLARFAPALAWATALAVCAGVGADLFWRF
                     TAPRAAALPVTPLTDPQEAGRAIAGRHLMGAGERAGPAADAVSPGRYTLQAVVTGAQG
                     RPGWAVIATDGGAQQGYVEGQEISSGVVLARVLPDAVEIALGPVRQTVRLAERAAGGA
                     LGVTPENPPPPAAAFLPQPNPSTPPVLPAAMQGQPPVTSDSPADLQPPSTPAGLPAPT
                     APSQ"
     gene            complement(14497..15354)
                     /locus_tag="PLH85_12735"
     CDS             complement(14497..15354)
                     /locus_tag="PLH85_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HNB65479.1"
                     /translation="MPSPVRPAAPALLHDLDALSRSSPQRRTLLRALLAGAAAPLAGC
                     GGSIDVGLGSVGTSTSSSSSSSTSSSTCSVIPEETAGPYPADGSNANSNGVVNALTLT
                     GVVRSDIRSSFAGASAVATGIPLTLTLQLVNAANSCANLAGYALYLWHCDRNGLYSLY
                     DSTLLNEDYLRGVQVTDSNGQVSFTTIFPGCYAGRMPHIHFEIYPSLSSAVSAAYKMR
                     TSQLTFPLTTINEAYATSLYSTSAANLSGMSFAADTVFSDGTSLQMASVSGSASSGYT
                     ASLVVAIST"
     gene            complement(15484..16236)
                     /locus_tag="PLH85_12740"
     CDS             complement(15484..16236)
                     /locus_tag="PLH85_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB65480.1"
                     /translation="MLPLLYRDDHLVAIHKPAGLLVHRSELDRHETRFAVQMLRDQLG
                     CHVHPLHRLDRGTSGVLLFALDRDTARLGSALFEHQAVDKTYLAVVRGHPPQSGRIDH
                     PLSRRRDEAEWRGDASSDTAQPAVTDFVRLATCELPFAVDRYPSSRYALLEVRPLTGR
                     RHQIRRHLKHIAHPLIGDATFGKGRHNRFFADHFGCARMLLACTRLRLIHPRSGAPLD
                     LVAPPEGDFLRLLDSLGWRPALPAILRTPPAA"
     gene            16523..>16637
                     /locus_tag="PLH85_12745"
     CDS             16523..>16637
                     /locus_tag="PLH85_12745"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB65481.1"
                     /translation="MSLPLIFPLHTRRAARRLASAGFTLLELLVVMTIIGLL"
BASE COUNT         2280 a         4836 c         7037 g         2484 t
ORIGIN      
        1 gatgaatggt ccttcggtca cggccgcgcg acgctgctcc agcccgaagc ttcgtgacgc
       61 ggcacggggc gcggatcgac ccgttttcgc cgtgggcgaa tgccttcatt gcgctcaagg
      121 cggtcccgct catggcgaga tttcatcgcg catgcgctgg ggagcgtttc tctcggtgcc
      181 ccgcgggtgc tcgtccatgc gcgcttcctg aaaggcgatg cgcgtgagtg cgtgggttcc
      241 gggcgcctct tggcgtgagg ggagggcatc agcgccttcg atgcattgat ccaacaattt
      301 gaatgctgca ctgcattcag aaatttccgt gcgggtacgt tatccagaga atgcccaaaa
      361 tgggcatgga tgaggaggac tcatcgaact catccggtgc gaagcacccg agagggcgcg
      421 tcgccaatct tgatcgtgcc gcgcattctg atcagaaagg aggcggtgcg tcgatagcgt
      481 gacgagagga actgcaatga aggctctgac ggacttgccc gtaggggtga gattgacgat
      541 tgtcatcgta gtggcgctat tgggcaccat tttgctcacg gcgctgtcgc tggtgcagtt
      601 tcacgcggtg gaggacaagg ctcaggcgct ggcgagccgc gtcaacaccg tccgtttggc
      661 tcaacgcatc acggtgaggg cgctggatgc ccgctcgcat ctggcgctgg cgctgcagca
      721 cgatccggag cgcccggaca tcgtccagtt gcactcccat caggtcggcg cccatctcga
      781 agcggtggcg cgcgatctga gcgatatccg ggatgcgatg gaggcgctgc atacgccgga
      841 ctccgggctg tcggcagagg aggccgctgc gttggccggc ctggagccgg aagtggcgcg
      901 cttcgagtcc cagggcgtgg ggccgatgcg tgaacggatc gaagccggtg ccttccacga
      961 tgcgaacgtg attctgctga agctgaccaa cccggcttac gaggctgtca agagtcaggc
     1021 cagcaccctg atcgaccgca tcgcagcgcg gacggacgcc gggcgcgccg agatggatgc
     1081 cgaagtgaag cgcacgacga tgctggtgat ttttggcggg gtggccttgg gtgttctgtg
     1141 cctgattgtc ggtttgcgtg taacgcgcag cgtcacgcgg cagatcggtg gtgagccgaa
     1201 ggaagccgtg cgtttgatgc aacaggtcgc cgaaggagat ttgtccatgg gggcggtgac
     1261 tgcgccagcc ggcagcatgc tcgccagcct gggcgcgatg gtggtgtcct tgcgggggat
     1321 cgtgagcaac atcgaccatg cggccagccg cgtggcggcc ggttcggagt gcatcggtaa
     1381 cgcgtcccga gcggtggccg acgcggcggg cgtgcagtcc gatgccacgg cgtcaatggc
     1441 tgcggcgatg gagcagatga cggtgagcat ttcccacatt tcggacagcg cgcgcgagac
     1501 ggcgaccgag tcggcccact ccgaccatct ggcggaggac ggcgcggtcc gcgtggccag
     1561 cgcccgccag gagatttcgc gcatcgctgt aacggtgacg gaggtgtcgt cggcgatccg
     1621 gcggctggcg gagcgggccg atcagatctc gtcgattgca ggcgtcatca aggagattgc
     1681 gggacagacc aatctcctcg cgctcaatgc ggccatcgag gctgcgcggg cgggcgagca
     1741 ggggcgcggt tttgcggtgg tggcggacga ggtgcgcaag ctggcggagc gcacggcggc
     1801 cgcgacggtc gagatcgaag ggacgatcac gacgatccag accgatacgg tacgggtggt
     1861 gaaggtgatg gattccgcca ttccgcaggt ggggagcggc gtagaggccg ccgaaggcgc
     1921 agcccaggcg ctgcaagcca tccgcgaggg cgcccacgtg gccttgcgtc gcatcgacga
     1981 ggtggccgac gccatgcggg agcacagcca gaccagcacg agcattgcac agcgggtaga
     2041 ggagatcgcc cggatgatcg aagagaccag cacggccatg cgtcagacgg cggataccgc
     2101 gagcgaactg gagaacgtat cggcgaccct ccggcagacg gtgggctgct ttcattgctg
     2161 agggagccgt ccggcacctt caggtgccgc gcgggcggat cgttcaggcc cgcaaaaggg
     2221 gcgggtgggc ccgttgtgaa tgacgccatc tgcgccaggg cgaaacgctg cgccggaggt
     2281 gcggcgggac gtgtggccgc gtgctgtgag ggtggggacg gccggtaagg ttccgtcccc
     2341 gccgacgtgc ttccgtctgc cgctcaggcg gtcagttcca gcatcttggc gaaggcggtg
     2401 gcgaactggg cgaggccgtc gtgctggagt ttggcgccga gcacgtccag gtcgagcccg
     2461 aagctcgcca gggcggcgat gtgggcgtcg gcgtcggcca cgcctgcggt gactgcgccg
     2521 ggggtgacgc ggccgtggtc gaggaaggcg gcaagggtgg cgtcgggcag ggtgttcacc
     2581 gtttccgggc cgatgagggc ttccacgtag agcagatcgg aataggcggc gttcttggtg
     2641 ccggtgctgg cccacagcat gctctgcggt cgggcgccac gggcggcgag ggcggcgaag
     2701 gcgtcaccgt ggaagcgcgc caggtattgc tggtaggcgc ggcgggcgaa ggcgacgccg
     2761 gtgcggccgc gcagggcgag ggcggcctcg ctgccgattt cttcgagctg cttgtccacc
     2821 aggctgtccc agcgggacag gaagacgctg gccaccgagc gcaccttgga gagatcgccg
     2881 cccttggcgg cgagggcttc caggccgcgc acgtaggcgg cggcgacggc ctccacgtgg
     2941 gcgggggaga acatcagggt gacgttgatg gagaggcctt cgccggtgag ggtttcgatg
     3001 gcgtgcacgc cggcggcggt ggcggggatc ttgaccagca ggttgtcgcg ggcgacggcg
     3061 gcggccagac ggcgggcggc ggcgacggtg ccggctgcgt cgtcggcgag ggcgggggag
     3121 acttccaggc tgacgtagcc ggcgtcgccc tggctggcgt cgaagaccgg gcggatcagg
     3181 tcgcaggcgc gctgcacgtc ctggatgaca agggcttcgt agcgggcttc ggcgtcgagg
     3241 gggcgggcgc gcagggcgtc gagatcggtg aggtagtcga gcccttcggc gatggccttg
     3301 tggaagatgg ccgggttggt ggtgacgccg gccacgccgt cggcgatgtg gcgggccagg
     3361 gtgccttcat tgatgagggc gcgggacagg ttgtcgagcc agatctgctg gccggcggcg
     3421 cgggcggctt gaatcggatt catgggcggt ggaaattcgg agggacgatg gaatggaagt
     3481 gaccgggcat tctaacggct gccgggaggc cagtggaggg tgtagcggcc ggcttgcggg
     3541 gtgggcgtgg cggccccgcc ggcgatggcg ggcaggcgcg acaggaaatc cgcgtcgccg
     3601 ctgatctggc cgtcgaaacg gggcggctga cccggtgccg cgctgccgcg accgtccagg
     3661 cggacctcgc cttcgagggt ggcgacggtg aattggagct ggccccggtc ggcctggatg
     3721 cggacttcgt aatcgccgaa gcggcggccg gggaacaggg cactgcgggc accgcgccac
     3781 aacaggcggg cctcgccgct gcagcggccg tctgccgcac aggcccagtg ggacgcgccg
     3841 agatccaggt cgccctgcca gccggcgcgg gcgatggggt ggggaatgga ggcgagcagg
     3901 gcgttggcgg gggtgtgcag atcgagttcc gagacgctcg cgccgccggg gcccaggtcc
     3961 acggtggcca ccgggcgggc gtcgctggtg aagcgccagc gcagggcgcc ctggaggagg
     4021 gcggtgggtt cgaactgcca ggccaggggc agccacgggg tgacggagcg accgcccgga
     4081 tcgctgctgg ccagcgtgcc gcggccggcc cacaggctgc cgtcggcgag cagcaggcgc
     4141 aggccggcgc ctgtggtgtg gctgaaggcg gcgtcgagca gggtggccgg cgcgcgcatc
     4201 agcgccagca cgagaaagat cagcaggaac agggcgagct tgcgcacggg gatcagggcg
     4261 cggcggggcc ggcgaggagg gcgtccacgc tggcctgact gccggcgcgc tggatctcga
     4321 gacgcagcac gcgcaggccc tgatcgctgt ggagggtgcc gagccagccg acgagggcgt
     4381 cgaaatcgcc gttgccgcgg atctgtacgc cgtcgccggt gctgtttacg gtgaccgcca
     4441 ggccgcgggc cttggcggcg gcgcccacgg cgtcggccag agctgggccg gcgaggggct
     4501 ggacgggcgg gcggccgcgc agggcggcga tctcggtggc ggcttcctgg gtctgctcca
     4561 gctgggcctg ggcgcggggc acttgccggt cgaggcgccg gcgttcctgg gcggaccagt
     4621 cggccaggcc gaagaccagg ccgagggcga ccacgctgcc ggcgatcagg atcagacggc
     4681 gctcccgttg ggagcaggcg ttccaggcgg tgagcaggcg cgcgaggggg gtgacgcggg
     4741 cggcggcgat gggggcgttc attgcaggct ctcccggcgc aggacgaggt gggtggcgtt
     4801 gccgtcgcgg cgcacgtcgg cgagcaggcc gcgggcggcg aggaggccgc cgagggccgg
     4861 cgcgcgggcg gcggcggggc cttgcaggct caggtcgagg cggccgtctt cgtagcgcag
     4921 ggcggcgacg ccgtcgaggg cgtcggtgcc gagggcgtcg gcggcctggg cgaggagggc
     4981 gagggcgtcg tcgtcccgca gcaggctgtg gcgtgcccgt tcctggttga gctgctgacg
     5041 catctgggcg gcgggggcga cgaccggggt gttggggaag gtggtggcat acacccgcgc
     5101 gatgcgcgtc tgcacgccgt cgaggctgtg gcgcagccac aggacttcgc ccacgttggc
     5161 gaggagccac acgcccagcg cggcggcggc gaggagggcg gcgggacgca ggcgggcgag
     5221 ccacgcggcg ccctgctcgc gggaggcgaa ttcgccgtgg agcagggagg ctgcgtggcg
     5281 gtcggcgccg gaccaccagc ggtagggttc ggccatgctc accggcaggc cggtctcgcg
     5341 ggccagcgcg gcggtgtcgg ggccgccgct gccgggcgcg agatgcacat gcagggcatc
     5401 gggcgcctga ttggcagcgc gggcggtggc ggcctgctgg gcgaggaggg ggatgaggag
     5461 gtcggcgtcg agggcgaccc cggtggtgac cgcactgcgc agcacggcgt ccggcccggc
     5521 cacggccagg tgccagcctt ccgggccggc gggggcggtc tgcacggcgg cgacgacccg
     5581 tcgcgggctg cggccgaggg cggcgagctg ggccagcagg ctgcgcaggc gctcgcgggc
     5641 gatcaccagc acgccgacga gatcccgttc gccgtcgccg gcggggcggc ggtggctgac
     5701 ggtgaggtgc tgggtgtccg ggtccttgag gaggcggtcc tccagggcat aggcgatgag
     5761 gcgcggcaga tcgcgacggg cggcgccggg cagggggact tccagccaca ggcactgggc
     5821 gccggtgagg acaacttcga cctcggcggc ggcgggccag tggcggggtt cggaatggcc
     5881 ttcggcggtg ggctggccgt cggggccgag gagcacccag gggcaggcgg ggtcggccgg
     5941 ccagtgctcg tcgagggcaa ggaggaggcg ggtggtcata gcagtttctg ccaaacgatg
     6001 tcgggccact tctggctgcg gtcgaggagc acttccatgc gcacggtgga gacgccgaag
     6061 cgggcccggc cggtggcgag gaagtggcgg ctggtggtgg cgatgcggga agtatcgggg
     6121 ctggcgttgc cggcgggaag gcgtgcggtg aagtcggcca cgtccttgaa ccaggcgcgg
     6181 gcgcgctggg cgaccacctg gcgggcgccg tcgaggccga ggccgggcac gacggcggcg
     6241 atgacctcgg cgggggcggt gtttacattg acggtggtgt cgggcggcag ggcggcaaca
     6301 aagggcgtca ggcgcgccac cagatcgggc gtgaagccgc ggatctgctt gagttcggcg
     6361 acggcggcga ggcgcgcgtt ggcggcgcga cgcggcgggt cttggctgcc ataccaggcg
     6421 gattcggcgc cgccgggcag gcgcggcagg tcgtccgcgt cgagccagtc gaggagggcc
     6481 gagctgaggg cgatggcgtc cgcgtcgccg acgccgacga ggcccagcag gcggcggaag
     6541 gcggccacgt cttcgacgga ggcgccgtcg gcgcggatca ggttgttgag gttgaggcgg
     6601 ccggagagat cctgcagttc gccgctgact tcgccttctt ccaccggcgt ggggggcacg
     6661 cgcacggccc aggattcgcc caggtggtcc acgctggtgc tgcgggcgtc ctcggcgagg
     6721 acgttgcggg cccagtccac ggcgccgcgg gcgagctggc gggcctgggc ctggtcgtga
     6781 cggccggcga cgctgtccag ggccacgccc aggtcgccca ccgcggcggc ggcgaggccg
     6841 gccaccaggg cgacggtgag gagggcgagg atcaccgcgg cgccgcgctg gacggggcgg
     6901 gacgatgggg cgacggcggg agcgacgggg cggcggctca tcgcagggcg atccagcggg
     6961 tgagggtgcc ggcgtcgggc aggtcgatct cgacgatgat ggcgtcgggc aggacatccg
     7021 gctggttggc gtcgggcggc caggcgtcgc tgcggaggtt gtccttgagg aagtgccagc
     7081 gtacctcgcg cacgttgtcg aggaggggct cgacggcggg ggcgtcggcc ggatcgcgac
     7141 cgctccagcg cagccacacg aggcggttgt cctcggcgcg gaagccgtag cggcggatgc
     7201 cgcgggtgtc gtcgaggcgc aggaactgga gctcgctgcc gccgccggca gaacgcagca
     7261 cggtcagcgc ggggctgcgg gcgacgccgt tctggccgcc gggggcggcg atctgcagca
     7321 ggtcggcgtc gatgcgctga acggcgcggc tgatgttgcg ccagcgctgg aagttctctt
     7381 ccaggcgggc gcggctggtg ctgacctcgg cgagggcgcg gtaggagagc acgcccagca
     7441 cggcgaagat ggccagggcg accatcagtt cgatgagggt gagcccgcgg gcggttgcgc
     7501 gtttcatcgc aggggcttga ccacgtagcc ggacaggcgc gcgatggcgt gttcgccgga
     7561 gtcttcgctg tacacgctga tgtcggcgcg gcggaacagg gggttgggcg tgccgcggat
     7621 ctcctcgcgc cagcggtagc tgcgtccggc ctgcacggcc tggccttcgt tgctgccggg
     7681 ttcgggccac acgtgggtgg cgcgcagctc ggcgaggcgg ttttcggcga tccagtcgcc
     7741 cagcaggcgg tcgcgcagtt cggcggagga ttgggtggcg acgcccaggg cacggaaggc
     7801 ggcggtcagg gcgatggcca ggatggtcag ggcgacgagg gtttccagca gcgtgaagcc
     7861 gcgcggggcg ggcttcatga gcggggaggc gcgtcgggtg gggtgaggag gacgtcgccg
     7921 ttggctttgc cggtgagcag ggcgatgccg cggggggtgg cgacgcgcag ttcgtattcc
     7981 ggggcctggg tgccgaactg caggcgcggc tgaggcgcgt cctggccgtc caggcgcagg
     8041 ccggaccagc tgaggccttc gggcagggtg cgttcggtga acaccggcgg gtcgatgagg
     8101 gggcgccagg tgtcgtcggc gtccagcgcg gcgaaacggt agccgtcagg caggaattcg
     8161 atggcgaggg ggcgtccgcg caccgcggcc cggtcggcgc tggcttccag caccaggcgc
     8221 aggcggcgca gggcctggtc cgcctcgcgc ccgcgcaggc tgtcgaggcc gacgctgatg
     8281 ccggtggcca tgaggcccat gatgaccagc acgaccatca cttcgatgag ggtgaagccg
     8341 gcggcgcagc gtggcggccg gggcgtgaac gcgtccgcgc gccgggcgtg ccgcgctgga
     8401 ggcaggccgg gcgtcgggat cgggaaaggg tgtgccgcgc tcacagactc cacgagccga
     8461 tgtcggcgtc gaagccttcg cccccctgca cgccgtcggc gccgaagctc atcacgtcca
     8521 cctcgccctt gaggccgggg atgacgtact ggtagggctt gccccagggg tcgttgggga
     8581 gcttttccag ataggacttc cagttctccg gcaccggggc gctgccgggc ttgaccacca
     8641 gggcctggag gccttgttcg gcggtggggt agcggcggtt gtcgagcttg tagagcttga
     8701 gggcctgcat gatggcgctg atgtcctggc gggcggcgac gacgcgggct tcgtccgggc
     8761 ggctcatgac cttgggcacc accagggcgg cgaggacgcc gaggatgacg atgacgacca
     8821 tcacttcgat cagggtgaag ccgcgggcgc ggcgcggacc ttgggacatg gcgggttcct
     8881 tatttcatca gggtgttgat ctcgatgatg ggctgcatga cggccatcac gatgaggagc
     8941 acgaagccgc ccatgaggag gagcagcacg ggctccagca gggcggtgag ggcggcggtg
     9001 cggttttcga gttcggcctg ctggaggcgg gcggcgcggt cgaggagttc gtccacccgg
     9061 ccggtggctt cgccgttggc cagcatgtgg atgagcaggg gcggaaactg gcggctggcg
     9121 ccgagggcac gggacagggg ctggccttcg cggacgcgtt cggcggcgac ggcgacagcg
     9181 tcggccaggg gcaggcgggc gatgacctgg cggccggcgt cgagggcggc gaggaggggc
     9241 acgccgctgc cggcgaggat ggacagggtg ctggcgaagc gggtcgcgtc aagggtgcgc
     9301 aggtgacggc cgaggaccgg caggcgcagc agccaggcgt cccagcggcg cttgaggtgg
     9361 ggttcgcgca gggccacgcg gccggcggcg aaggcgccga tgagggcaag gaccatcagc
     9421 cagccccagt cccgcagcac ggcgctggcg gcgatgaggg cgcgggtgag gaggggcagg
     9481 gcctgcttgc tctgctggaa gacggagacg acctggggca ccacgtaggt catcaggccg
     9541 atgatgacca tcagcgccac gccggcgacg atggcggggt agagcagggc ctggagggtc
     9601 ttggtgcgca gggcgttgct gcgttccagg tagtcggcga gctggttcat gacccgcgcc
     9661 agttcgccgg atttttcgcc ggccgcgatg gaggcgcggt agagggtgga gaaggcctgg
     9721 ggatagcggt cgagggcggc gcgcaggctg tagccggcga ggatctcgga acgcacgccg
     9781 gcgaggagct ggcgggtggc gtcggtttcg gcctgttcga tgagggcggc gagggcctgt
     9841 tcgacggtga ggccggaggc gagcagggtg gcccactggc gggtgagcag ggtgaggtcg
     9901 gcgtccttga ggcgccgccg ggcgctgcgg gcgccgccac cctggctgac gctggcgact
     9961 tcgaggggga acaggccgcg ctcgcgcagc aggccgcggg cggccttgcc ggtgtcggct
    10021 tcgaggacgc cggcggtctc cttgccggcg gcgtcgaggg cgcggtagcg gaaggcggtc
    10081 atggggcggg gccgggcggg ttcagtcgcg ggtcacgcgc aggagttcct cggccgaggt
    10141 gatgccgtcg gcgaggaggc gcaggccgtc gtcgcgcagg ctgcgggtgc cctgggcgcg
    10201 ggcgtgggcg cgtagcgcgg cttcgtcggc ggcggtgtgg atgaggcgct gcagggcctc
    10261 gtcggcgacc atcagctcat agaggccggt gcgcccggcg tagccgctgc gggcgcagct
    10321 cgggcagccc ggcgcatgcc acaggcgggc cggcgcggcc ccgagggcgt cccggtcggc
    10381 ggcgtcagcg ggggcatcgc ggcggcaggc ggggcacagc ttgcgcacca ggcgctgggc
    10441 gagcacgcca cgcagggtgg aggccagcag gaagggctcg acgcccatgt ccaccaggcg
    10501 ggtgacggcg gaggcggcgt cgttggtgtg cagggtggcg agcacgaggt ggccggtgag
    10561 gctggcctgc acggcgatct gggcggtttc caggtcgcgg atctcgccga tcatgatcac
    10621 gtccgggtcc tggcgcagga tggcgcgcag ggccttggcg aagctgaggt cgatgcggct
    10681 gttgacctgg agctggccga cgccggggag gtcgtattcg accgggtctt cgacggtgac
    10741 gatgttgagc gtgcgggcat ccatggcctg cagcgcggcg tagagggtgg tgctcttgcc
    10801 ggagccggtg gggccggtga cgaggaggat gccgtggggt tcgcgcagca gggtttcgaa
    10861 ggtgccgcgg gtgtcggcgg ccatgcccag gctctccagg ccgcgctggc cgctgccctt
    10921 gtcgagcagg cgcagcacga tgcgttcgcc gtgggtggtg ggcagggtgg acacgcgcac
    10981 gtccacctgg cggccggcca ggcgcagggc gatgcggccg tcctggggca ggcgcttctc
    11041 ggcgatgtcg aggctcgcca tgatcttgat gcgcgaggcc atggcggcgt gcagggcgcg
    11101 gtggggctgg gccacgtcca ccagcacgcc gtcgcggcgg aagcgcacga cggagtggcg
    11161 ctcgtagggt tcgatgtgga tgtcgctggc ggcctggcgc acggcctggg tgaggagggc
    11221 gttgatcatg cggatgatcg gcgcgtcgtc ctgggtttcg agcagatcct ccacggcggg
    11281 cagctcggtc atgagctggg tgaggtccac ttcctggccc acgtcggcga cgacctcggc
    11341 ggcgttgtcg tcggcgccgg aataggcctc ggcgaggcgc gcctcgaagg cggcgcggcc
    11401 gacgctctcg aggcggatcg gcacgcccag gctgcgccgc acttcggcga cggcggcgag
    11461 gtcggcgcct tcgcgcagga tcaggtcggc gtgctcgggt ccgtggctgg cgacgaagac
    11521 gccgtggctg cgggcgtagg cgtagggcag cagcggcgcg ctcattgcgg ggccggacgg
    11581 tcggacggct ggggcacggt ggtgggcgtg gccggcggcg gcgagccggc gggcggcagg
    11641 gaaggcaggc cgcggtcgaa cttgaagcgc tgctcctcgc tgtccatggt ccgctgctgg
    11701 ccgatgatgt agtcgtagcg gtcggacgag atgccccgga tggcggcggc atcgcgcagg
    11761 atgacggggc gcaggaagac gatcaggttg gtcttggtgc gcttgcgcgt gtcgtagcgg
    11821 aagaaggcgc ccaaataggg gatgtcgccg aggacgggga ccttctcgat ggtgccgctg
    11881 aagctgtctt ccaccaggcc gccgagggcg atggtggcgc cgtcgtccac cagtacggtg
    11941 gattcgatgg agcgcttgtt ggtggtgggg ccgtaggtgt tggcggtggt ggtgtcgatg
    12001 accgaggagg cttcctggta gatctgcatg cgcaccaggc cgccttcggt gatctgcggt
    12061 ttgaccttga gggtcaggcc cacgtccttg cgttcgatgg tggtgaaggg gttggtggtg
    12121 gtggtgccgc cggtggtggc gtaggagccg gtgacgaagg gcaggttgcg gccgacgacg
    12181 atcttggctt cctcgttgtc cagggtgacc aggttgggcg tggacaggat gttggcgcgg
    12241 ctgtcggatt cgaggaagcg ggccagcatg ttgaggttgg cgatggtctt gccacccacc
    12301 gtgacgctgc cgctgcccag caccaggttg aggccgttgc ccggcgaggt gacggtcccc
    12361 gagcccagcg cagtggccag ggtgagcagg ttggagccgc tgctgctgga ggtgaaggcg
    12421 gtgccgccgt aggccgaggt ggtgccgacc ttgccggcct gccactggat gccgatctcg
    12481 gcagccttgt cggtgctgac ttcggtgatg agggcctcca cgtacacctg ggcgcggcgc
    12541 cggtcgagct ggtccaccac ctggcgcagg ttggtgtaga tggtgtccgg cgcggtgatg
    12601 atgagggcgt tgttcatcgg gtcggcctgc acgacgccgc cgccggtggc gttgggcacg
    12661 ccggtgctgg tgccggagct gccggagaag ttgccgccgc cggtggtggt ggccgtgttc
    12721 tgaccgaagg tgctgccggt ggtgccgctg ttgaaggtgc cggcgccggt gggggcgaag
    12781 ctggacgagg tggcggtgct gccgctcgtg ccggtggtgc cggattcgcc ggagagcacc
    12841 gcgcgcaggg tctgggcgac cttggtggcg tcggcgttct tgaggtagac gacgtggatg
    12901 ttgccggccg cgccgggctg gtccaggttg cccacgagct ggcgcacggc ctggatcttg
    12961 gacgggttgt cggagcggat cagcaggctg ttggtgcggg cgtcgccgat cacggtgacg
    13021 cgctggctgg tgtcgccggt ctggcccgcc gcgccggcgg ccgagccgtc gccgagcagg
    13081 cggttgatgg tggcggccag gtcgagggcg gaagcgtgct tgaggggcag catgcgcatg
    13141 tcgccctggg gcacgtcgat ggattcgatg atctgggcga tgcggccgag gttctcggcg
    13201 tagtcggtga ccaccagggt gttgttgccc gggtaggcgg tgacggtgtt gttgggggag
    13261 atcagcgggc gcagcacggg gacgatctgg gcggcggatt cgtgcttgag ggtgaacacc
    13321 tgggtggtga ggcggtcacc gccaccgctg ccctggcctt tgccgaccgg caccgcgtgg
    13381 agcttggcgt cggcttccgg gacgatcttg gtcacgccgt cgccctcgat ggccgcgtag
    13441 ccctgcaggc gcagcgcgga gagcaggatg gggtaggtgt tgcggcgttc gacggggcgc
    13501 gcggtgacga tgttgagggt gcccttgacg cgcggatcga cgacgaagtt gcgtccggag
    13561 atcttggaga ccgcctggat caccgcgccg atgtctgcat tgacgaaatt gagggagacc
    13621 cggttgtcgg cgtcgtcgcc cttggccgcc agggcgggcg gcaggggcag ggcgagggcg
    13681 gcagccagac aggcggcaag gatccggcgg gcagggttca ttggctcggg gcggtgggcg
    13741 ccggcaggcc ggcgggtgtg gacgggggct gcagatcggc cggcgagtcg ctggtgacgg
    13801 gcggctgacc ctgcatcgcg gccggcagga ccggcggcgt cgaaggattg ggctggggca
    13861 ggaaggcggc ggcgggcggc ggcgggtttt ccggggtgac gccgagggcg ccgccggcgg
    13921 cccgttcggc cagacgcacg gtctggcgga cggggcctag ggcgatctcg acggcgtccg
    13981 gcagcacgcg ggcgagcacc acgccgctgc tgatctcctg gccttccacg taaccctgct
    14041 gggcgccgcc gtcggtggcg atcaccgccc agccaggacg gccctgggcg ccggtgacga
    14101 cggcctgaag ggtgtagcgg cccgggctca cggcatcggc ggccggaccg gcgcgctccc
    14161 ccgcgcccat caggtggcgt ccggcgatgg cacggccggc ctcctggggg tcggtcagcg
    14221 gggtgaccgg cagggcggcg gcgcgcggcg cggtgaaacg ccagaagagg tcggcgccga
    14281 cccccgcgca cacggcgagc gccgtcgccc acgcaagcgc cggggcgaag cgggcgagag
    14341 ggagccgggt gagccgggag aggtcgagac gttcggggag gttcatcggg cgctgcaaca
    14401 ggggccagca atcttagccg gcgaatgtta ccggcaaaag ggccccggtg tcgcgccgcc
    14461 aaacggacgg cgtgccagtg ttcgcggggg actgcctcag gtagagatgg ccacgaccag
    14521 ggaggccgtg tagccgctgc tggcgctgcc gctgacactg gccatctgca ggctggtgcc
    14581 gtcgctgaag acggtgtcgg cggcgaagct catccccgac aggttggcgg cgctggtgct
    14641 gtaaagactt gtggcgtagg cctcgttgat ggtcgtcagc gggaaggtga gctgggaggt
    14701 gcgcatcttg taggcagcgc tgaccgccga ggacaggctc gggtagatct cgaagtggat
    14761 gtggggcatg cggccggcgt aacagccggg aaagatggtg gtgaagctga cctggccatt
    14821 gctgtcggtg acctgcacgc cgcgcaggta gtcctcgttg agcagggtgc tgtcgtagag
    14881 ggagtacagg ccgttgcggt cgcagtgcca caggtagagg gcgtagccgg ccaggttggc
    14941 acagctgttg gcggcgttga cgagttgcag cgtgagggtc aggggaatgc cggtggcgac
    15001 ggcgctggcg ccggcaaagc tgctgcggat gtcgctgcgc acgacgccgg tgagggtcag
    15061 ggcattgacg acgccattgc tgttggcatt ggagccgtcg gccgggtagg ggccggcggt
    15121 ctcttcgggg atcaccgagc aggtgctgct ggaggtgctt gagctgctgc tggacgaggt
    15181 gctggtgccg acgctgccga gccccacgtc gatgctgccg ccgcagccgg cgaggggcgc
    15241 ggcagcgccg gccaacaggg cgcgcagcag ggtgcggcgc tgcggcgagc tgcgcgagag
    15301 ggcgtccagg tcatggagca gggcgggggc ggcggggcgt acaggggagg gcatcgggac
    15361 ggctccgggt cggaagtaag gccctcggaa gggcgtgtga cggatgcgaa aagtgtacgc
    15421 agccgacccg gtgtgccgga gatgccggtg ggtaaaagcg ggtaaagata cgttggactg
    15481 tgttcaggcg gcggggggcg tgcgcagaat ggcaggcagc gcgggccgcc agccgaggct
    15541 gtcgagcagg cggaggaagt cgccctccgg gggcgcgacg agatcgaggg gcgcgccgct
    15601 gcgtggatgg atgaggcgca ggcgcgtgca cgccaggagc attcgcgcgc agccgaagtg
    15661 gtccgcgaag aagcggttgt ggcggccctt gccgaaggtg gcgtcgccga tcagggggtg
    15721 ggcgatgtgt ttgagatggc ggcggatctg gtggcgacgc ccggtgagcg ggcggacttc
    15781 gagcagggcg tagcggctgc tcgggtagcg gtctacggcg aagggcagct cgcaggtggc
    15841 gaggcggacg aagtcggtga cggcgggctg ggcggtgtcg ctgctggcgt cgccgcgcca
    15901 ctcggcctcg tcgcggcggc ggctgagggg gtggtcgatg cgcccgcttt gcggcggatg
    15961 gccgcgcacc acggccaggt aggtcttgtc gaccgcctga tgttcgaaga gggcgctgcc
    16021 cagacgggcg gtgtcccggt ccagggcgaa gagcagcacg cccgaggtgc cccggtcgag
    16081 gcggtgcagg ggatggacgt ggcagcccag ctggtcgcgc agcatctgca cggcgaagcg
    16141 ggtttcgtgg cggtcgagtt cgctgcggtg gaccagcagg ccggcgggtt tatggatggc
    16201 gacgaggtgg tcgtcccggt acaggagggg cagcatgtcg ggcggtcgga aaggcgccgc
    16261 gatcgggcac tcgggcaggg gcacgcatca taccgcggca ggggtacgga ccgctttcgg
    16321 ggcggaccgg tgacaaatgc ctttcttgca cgacaaatgg atgtactaaa atgcggcgcg
    16381 ctcgggcgga gggtgcctgt ccgctggcag aacccgtccg tacggcgccg gcgctccagt
    16441 gcgctgcgca ccggtcgtta atgattccga tgccggtcgc ggccggttcg gccttctttt
    16501 cttcctgttc caggtcgtgt tcatgagtct cccgctgatc tttcccctgc acacccggcg
    16561 ggcggcccgc cggctggcca gtgcggggtt cacgctgctg gaactgctgg tggtgatgac
    16621 catcatcggc ctcctgg
//
LOCUS       DAOQMM010000083        16571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1335_length_16571_cov_41.695507, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000083 DAOQMM010000000
VERSION     DAOQMM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16571
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_16571_cov_41.695507"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..852
                     /locus_tag="PLH85_12750"
     CDS             190..852
                     /locus_tag="PLH85_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNB65482.1"
                     /translation="MAYDLTKPYGRFMAYMNMQLEDHGIVRSIYNNFYALPGGMYRCS
                     QPSPAQIRKYHRLYGIKTIINLRGENPHGSYDLEVDTCRELGIELINFNLYSRDMPSV
                     EEVLGARDLFERIAYPALMHCKSGADRAGLGATFYRHFRLGEPLDQIRELSPKYGHFT
                     IARTGILDFFLACYQADNARAPIDFLTWVQTRYDQKALTEKFDARSRLGTWFVDKVLR
                     RE"
     gene            849..2609
                     /gene="msbA"
                     /locus_tag="PLH85_12755"
     CDS             849..2609
                     /gene="msbA"
                     /locus_tag="PLH85_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNB65483.1"
                     /translation="MKASLQLYRRLLGTIVPYWRVVAISIAAMVLAAGLEPVMPTLLK
                     SLVDKSLIQKDPIAAWQLPLFLMLTFLGKGIAEYVTNVASQWVANKAVADLRQQVFHH
                     QTALPLATHLAEPPGRMLSRLLYDIPQVGQALSTAWIIVIRDSLVIVGLTGYLIYTAW
                     ELTLLILAIAPVVAWIIRVASARLRTSNQDIQTITGRLTGMVDASLVGVKEIKIFGAR
                     DYEAARFAQVVDRLRKETMRAVRVSSVNVPLVQVLAAMAVSLVIYVASSLSANDRLSP
                     GEFVAFVTAMSMLFEPIRRLTNVNATIQSGLAGAQSIYELLDSPPEPEQPAGPTATLP
                     APPGGGSEVRFEHVSFTYPGQPAPAVADLSLIVPPGHTVALVGGSGSGKTTAVSLLAR
                     FFNPEAGHILLDGAPIDALPLSALRARIAMVGQQVVLFDDSVAANIAYGRPDIPRAAI
                     EHAARAAHAWEFIEKLPQGLDTPIGENGARLSGGQRQRIAIARAFLKDAPVLILDEAT
                     SALDTESERQVQAALGTLIKGRSTLVIAHRLSTIEGADTIVVMDKGRVVEQGRHAELL
                     ARDGAYARLYRMQFQDEAPA"
     gene            2706..3971
                     /gene="hldE"
                     /locus_tag="PLH85_12760"
     CDS             2706..3971
                     /gene="hldE"
                     /locus_tag="PLH85_12760"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: protein motif:HMM:NF008454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HNB65484.1"
                     /translation="MTPTLPDFSRGRVLIVGDVMLDRYWHGATSRISPEAPVPVVKVD
                     MDEFRAGGAGNVALNVATLGGGVSLVGLVGDDESAGLLRARLEAGRVACHFVPVAGSH
                     TITKLRVISRHQQLIRLDFEDGFTRAEAAAVTAAVRAGLDGVGAVVLSDYAKGTLADA
                     RALVHAARERGLPIVVDPKGTDFERYRGASVLTPNLSEFEAVVGRCADEASLRARGTA
                     LRDALDLDALLVTRSERGMTLLQRGHAPLDLPTHAREVFDVTGAGDTVVALLAAGLAA
                     GLPLAEATALANVGAGVVVGKLGTATVSVDELAAALHAPAGGARGIVDEPRLAAAVAQ
                     ARDAGERVVLYPAAFDGLRPADLRALQAARRQGDRLVAAVLNDAAATCALGERLALAA
                     ALEAVDWTIACDESPAELAARIGAELAVS"
     gene            3982..4929
                     /gene="rfaD"
                     /locus_tag="PLH85_12765"
     CDS             3982..4929
                     /gene="rfaD"
                     /locus_tag="PLH85_12765"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNB65485.1"
                     /translation="MIIVTGGAGFIGSNIVQGLNARGVTDILVVDDLSDGRKCLNLSD
                     AHIHDYLDKDDFLARLQAGESFGPVEAVFHQGACSATTEWDGRFVMNVNYAYSKAVLR
                     WCVERKVPFYYASSASVYGEGPVFKEDRAHEHPLNAYAYSKFQFDCHLRPQLAGFRSP
                     VVGLRYFNVYGPREQHKGSMASVAYHFNTQLKEGDRLRLFEGSDGYGPGEQRRDFIHV
                     DDVVAVNLWLLEHPEVSGIYNCGTGRAQPFNDVARATIAFHGRGHIEYIPFPEHLKGR
                     YQSYTQADMGALRAAGYDRPFLSVEEGVARYMAWLAKRS"
     gene            4942..5973
                     /gene="waaF"
                     /locus_tag="PLH85_12770"
     CDS             4942..5973
                     /gene="waaF"
                     /locus_tag="PLH85_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNB65486.1"
                     /translation="MAPRRILVVGPSWVGDMVMAQSLFMTLKARHTLVEIDVLAPGWS
                     LPILARMPEVRRGIAMPLGHGQFGLRTRWQLGRQLAQDRYDQAIVLPGSFKSALAPFF
                     AGIPRRTGFRGEMRFGLLNDMRRLDKRALPMTVQRFVALGLPPDTPQPPPTPTPRLQP
                     DPAGFARLRAQLDLLADRPGVCFMPGAEYGPAKQWPIPSYGELGRRLVRQGFQVWVLG
                     SAKDKPAGDEIATLAGEGAFNLCGRTELADAVDLLATARAAVTNDSGLMHVAAALGTP
                     LVALYGSSTPHHTPPLSDAAQVIYLGLECSPCFKRTCPLGHTRCLTGIGVERVAEALA
                     PALHATAPR"
     gene            5970..7205
                     /locus_tag="PLH85_12775"
     CDS             5970..7205
                     /locus_tag="PLH85_12775"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB65487.1"
                     /translation="MNTRPPFQDRAADAARWLCVASAFAVPLPAAWLSFTTGLFLIAW
                     GLAGRLPLRWATIRQHPVGRLSLVLLLWMAVAIAWAPVEPKVGLAHWWHYRELLIVPL
                     MLSLFDADTARRWQPRVFTAFFIGFGIALVTSFLRWTGVLPDMGFKGLYAGFGGRTGF
                     SMLLAWAIFCAVEFGRDQPRFRLAWAALALVCFANLFFINTGRTGQIAFFTLVPLSLY
                     HLAGRRGLVAGLLAIPVLAAGMYAASPAVRARIAESAEHIAAYEAGHPYTGDGLRLEF
                     IANAADFIRQHPVFGGGTGSFAAEYKVVAEREGMTGERVADNPHNEYLMVWSQQGLPG
                     LALLIALFLTQWRLSARLDDLPARLNRGLVLLIAGGDLVNSLILDNLEGHLWALLTVA
                     LTAGIGADRAAPSPTESSR"
     gene            7207..7953
                     /locus_tag="PLH85_12780"
     CDS             7207..7953
                     /locus_tag="PLH85_12780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB65488.1"
                     /translation="MARPTLSVIIIARNEAALIAKCLDSVTWADEIVVVDSGSTDGTP
                     EICRRYTDKVFVTPDWPGFGPQKNRALDLATSDWVLALDADETISIPLATEIAATIAL
                     TPHDVYAVKRLSSFLGRPIRHGDWWPDEVPRLFRRGCARFSDDPVHERLVFEGRPARL
                     RALMMHDSIRSLDQMCAKMNHYTSAGAERLARAGKRGGLGKAIRHGLWAFLRAYLLKR
                     GFLDGREGFILAVSAAESAYYRYLKRLYLP"
     gene            7950..8939
                     /locus_tag="PLH85_12785"
     CDS             7950..8939
                     /locus_tag="PLH85_12785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB65489.1"
                     /translation="MSASADLAARRFSVVIVNYNGGQMLLDCVRSVRSGLLPADRIIV
                     VDNGSRDDSMARLEAAEPGCVILRNGCNAGFARAVNRGLAQVRSEFALLLNNDARLAP
                     DAWDALAAAFDTLPRAAFLGGRLLYEDGRLQNAVAAFPRLSAELLPRALLRALWPTRY
                     RGRPTGDAPLRVESIIGALFAVRMAAVAEFGPLDEDFFFFLEETEWCHRAHTRGWETW
                     HIPAARAVHAQGATAKKHNALARIEYHRSRLLYFRKTAPGRYALSYAIALTKAAFNAL
                     GNALLAGLTLGLAPRVRAKAGVDLKILAWYLQGRPADVGLPDKCPPGRPLPGA"
     gene            8946..9920
                     /gene="waaC"
                     /locus_tag="PLH85_12790"
     CDS             8946..9920
                     /gene="waaC"
                     /locus_tag="PLH85_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HNB65490.1"
                     /translation="MKLLVVKTSSLGDVIHMLPAVTDAARARPGLTVDWLVEESFAAI
                     PPWHPAVRRVLPIAMRRWKKQWRRATTWAQLRAARAALQATDYDWVVDTQGLLKSALW
                     TRWARGPRCGYDAASAREPLAARFYERHYAVPREMHAIERNRRLLAQALGYTLDDPRP
                     DYGLAGLAARLPAPDVSLPARYVVGLHGTSRADKEWPVEHWKALARALGGAGAPLVMP
                     WGNEAERQRAEAIAAAAPGALVLPRLGLDALAVILDRAAAVTGVDTGLMHLAAGLGKP
                     GLALYTATRPALTGVVPDRHSRSALENRERPDALGADAVIAGMLARLA"
     gene            9930..10655
                     /locus_tag="PLH85_12795"
     CDS             9930..10655
                     /locus_tag="PLH85_12795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB65491.1"
                     /translation="MSAILPILMYHQIDATPPRGTPLRGLTVTPGSFARQMALLRLLG
                     YRGVSMHDLEPYLRGERRGRVVGITFDDGYRNNLEHALPVLQKHGFSATCYAVSDPFE
                     GRNAWDEGMGMPQKPLFTPDDMRAWVAGGMELGAHTRHHADLTRIDDATAEDEIAGCK
                     RELEAIIGREVRHFCYPYGHYGPAHRDMVRAAGYRSATTVERSRARSSDDPFELPRVM
                     IARTNHLALFWMKIATGYEDSRG"
     gene            10652..11800
                     /locus_tag="PLH85_12800"
     CDS             10652..11800
                     /locus_tag="PLH85_12800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB65492.1"
                     /translation="MTRRLRIAVLNRHFGRRFGGAESYSVALVEALAARHELHVFAQE
                     FDHPGIGIALHRVPRPLAKPRWLNQLWYAAWTWQATRRGFDVVHSHENTWHGQIQTVH
                     VRPLRVGLFHGLSAGRRILKTLQILTSPRLLAYWWLEGSRFRPRPDRRIVASSTSVRD
                     EVLQAYPHAAGMMSVIPPGTLLPAGPADPAAARRQLKLPADVPLALFAGNDYVKKGLP
                     ALLEALTRLPADLHLAVAGNPAPIAAFRAQAKQLGVGARVHFLGSLRDMVPAYTAADL
                     LVHPTTEDTYAMVVLEALAHGLPVIVSGPAHCGIATELRDGVDALLVDDPRDADALAR
                     AIARVRTDVGLAAALGLAGRAFAAARDWDARAAVQEALYFEAAGNATG"
     gene            complement(11973..13433)
                     /gene="gatB"
                     /locus_tag="PLH85_12805"
     CDS             complement(11973..13433)
                     /gene="gatB"
                     /locus_tag="PLH85_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNB65493.1"
                     /translation="MSRTDWEVVIGLEIHTQLNTASKIFSGASTAFGAAPNVQASAVD
                     IALPGVLPVLNRGAVERAIRFGLAIGATIAPRSIFARKNYFYPDLPKGYQISQYELPV
                     VQGGAITLLVPDGQGGHYEKRVRLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLE
                     IVSEPDMRSAAEAVAYAKALHALVRWIDICDGNMQEGSFRCDANVSVRPRGQAEFGTR
                     REIKNLNSFRFLQQAIDYEVQYQIELIEDGGKVQQATVLFDPDSGETRAMRSKEDAHD
                     YRYFPDPDLLPLVIDSAWIERVKGELPELPAALSARFEGEYGLSAYDATTLTASREIA
                     EFYLAAVAVAGTALAKPCANWVMGDLAARLNKAELDIAASPVSPVQLAGLVQRIADNT
                     ISNAIGKKVFEALWNGEGPSADAIIDAQGLKQVTDSGAIEALIDEVLAANPKSVDEYR
                     AGKEKAFNALVGQCMKASKGKANPTQVNALLKQKLG"
     gene            complement(13454..13954)
                     /locus_tag="PLH85_12810"
     CDS             complement(13454..13954)
                     /locus_tag="PLH85_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65494.1"
                     /translation="MFHRFFPSLLAALIAGCASVELPRLPGARDEPPAARVPAAPSAD
                     SGKLKPMPLRTFTVSTQCSFKDETGNFGKATVDVQDSKVRALAVELVMPRRGTCRYDL
                     TQFRQTQALPSVELAGGRDCRIRMWEQGETITVSFANCPQHCNPASATDYVWPLLIDR
                     PTGQCD"
     gene            complement(13960..14331)
                     /locus_tag="PLH85_12815"
     CDS             complement(13960..14331)
                     /locus_tag="PLH85_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65495.1"
                     /translation="MPYTRTQIQAFVGGELTTYLPQRDHRILAGCFARDLNPKAPDIV
                     FAGNKAKVAHKARLLARQPDNCFPVFVRDNKRAPYFFQGYFAYERLSDLAEDIRSAEA
                     LSGRYNQLSCVLHLKRCPDRE"
     gene            complement(14361..15812)
                     /gene="gatA"
                     /locus_tag="PLH85_12820"
     CDS             complement(14361..15812)
                     /gene="gatA"
                     /locus_tag="PLH85_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNB65496.1"
                     /translation="MIESSLKDIAAALRARRVSAVELATDSLSRIDALNPRLNAFITV
                     DRHGALAAAQAADARLAAGDAGPLTGIPLAHKDVFCTEGVLTTCASKMLANFVSPYDA
                     HVVSLLKAAGAVMVGKTNMDEFAMGSSNENSHFGPVRNPWNTDTVPGGSSGGSAAAVA
                     ARLVPIATGTDTGGSIRQPAALCGITGIKPTYGTVSRYGMIAYASSLDQGGAFARTAE
                     DCAVLLSAMAGHDPRDSTSLERPAEDYGRDLAQPLAGLRIGLPTEFFAEGMSDDVRAV
                     VDAALDQYRALGATTVEVSLPNARLAIPAYYVIAPAEASSNLSRFDGVRYGHRAAEYT
                     DLMDMYCKSRAQGFGGEVQRRILVGTYVLSHGYYDAYYIKAQKLRRLIAEDFQSALAQ
                     CDVIAGPVSPTVAFGIGEKSDDPVQMYLSDIYTIAVNLAGLPGLSHPAGFGAGGLPVG
                     LQLIGNYFAEARLLNVAHQFQQATDWHARAPQL"
     gene            complement(15874..16161)
                     /gene="gatC"
                     /locus_tag="PLH85_12825"
     CDS             complement(15874..16161)
                     /gene="gatC"
                     /locus_tag="PLH85_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNB65497.1"
                     /translation="MSLTVDQVRHIAKLARLEVSTDDLAATQAKLNGIFGLIEEMQAV
                     NTDGVEPMSHPQDLAQRLRADAVTESDRRDAFQAIAPQTEGGLYLVPKVIE"
     gene            16289..>16571
                     /locus_tag="PLH85_12830"
     CDS             16289..>16571
                     /locus_tag="PLH85_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNB65498.1"
                     /translation="MFGSLRSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRTE
                     GGPNAKKTILAVGMAAKQMLGKTPGNITAIRPMKDGVIADFTVTEQMLKQ"
BASE COUNT         2379 a         6270 c         5382 g         2540 t
ORIGIN      
        1 ggcaccaggc cctcttcgtc catctcctct tcccgacccg gcgcgcgtgg catggcgtgg
       61 ctctcccctg gatggtctga ccagtccagt atgatccccg caccgcccgt cgccaagccg
      121 tcgccctgcc ggagagaaat ccaagggtct gtataatcgc gcttttgccc cctccggacc
      181 ccgcgccccg tggcctacga cctcaccaag ccctacggcc gcttcatggc ctacatgaac
      241 atgcagctcg aggaccacgg catcgtccgc tcgatctaca acaacttcta cgccctgccc
      301 ggcggcatgt accggtgcag ccagccgagt ccggcgcaga tccgcaagta ccaccgcctc
      361 tacggcatca agacgatcat caacctgcgc ggcgagaacc cccacgggtc ctacgatctg
      421 gaggtggaca cctgccgcga gctgggcatc gagctgatca acttcaacct ctactcccgc
      481 gacatgccct cggtggagga ggtgctgggc gcgcgcgacc tcttcgagcg catcgcctat
      541 cccgcgctga tgcactgcaa gtccggcgcc gaccgggccg gtctgggggc caccttctac
      601 cgtcacttcc gcctgggcga acccctcgac cagatccgcg agctgtcgcc caagtacggc
      661 cacttcacca tcgcccgcac cggcatcctg gatttcttcc tggcgtgcta ccaggcggac
      721 aacgcccgcg cgcccatcga cttcctcacc tgggtgcaga cccgctacga ccagaaggcc
      781 ctgaccgaaa agttcgatgc ccgcagccgc ctgggcacct ggttcgtgga caaggtgctg
      841 cggcgcgaat gaaggcctcc ctgcagctct accgccgcct gctcggcacc atcgtcccct
      901 actggcgggt ggtggcgatc tccatcgccg ccatggtcct cgccgccggc ctggagccgg
      961 tgatgccgac cctgctcaag tccctggtgg acaagagcct gatccagaag gaccccattg
     1021 ccgcgtggca gctgcccctg ttcctgatgc tcaccttcct cggcaagggc atcgccgagt
     1081 acgtcaccaa cgtggccagc cagtgggtcg ccaacaaggc cgtggccgac ctgcgccagc
     1141 aggtcttcca ccaccagacc gccctgcccc tcgccaccca cctggccgag ccgcccgggc
     1201 gcatgctgtc gcgcctgctc tacgacatcc cccaggtggg ccaggccctg tccaccgcgt
     1261 ggatcatcgt catccgcgac tccctggtga tcgtcggcct gaccggctac ctcatctaca
     1321 ccgcgtggga gctgaccctc ctgatcctcg ccatcgcccc ggtggtggcg tggatcatcc
     1381 gtgtcgccag cgcccgcctg cgcacctcca accaggacat ccagaccatc accggccggc
     1441 tcaccggcat ggtggacgcc tccctggtgg gcgtgaagga gatcaagatc ttcggcgcgc
     1501 gggactacga ggcggcccgc ttcgcccagg tagtggaccg cctgcgcaag gagaccatgc
     1561 gcgccgtgcg cgtctcctcg gtcaacgtgc ccctggtaca ggtgctcgcc gccatggccg
     1621 tgtccctggt gatctacgtg gcctccagcc tgtccgccaa cgaccgcctg tcgcccggcg
     1681 agttcgtcgc cttcgtcacc gccatgtcca tgctcttcga accgatccgg cgcctgacca
     1741 acgtcaacgc cactatccag agcggcctcg ccggcgccca gagcatctac gagctcctcg
     1801 attccccgcc ggaaccggaa cagcccgccg gcccgacggc gaccctgccc gcgccccccg
     1861 gcggcggcag cgaggtgcgc ttcgagcacg tgagcttcac ctatcccggc cagcccgccc
     1921 cggccgtggc cgacctcagc ctgatcgtgc cgcccggcca caccgtcgcc ctggtgggcg
     1981 gctcgggcag cggcaagacc accgcggtga gcctgctggc gcgcttcttc aacccggaag
     2041 ccgggcacat cctgctcgac ggcgcgccca tcgacgccct gcccctcagc gccctgcgcg
     2101 cgcgcatcgc catggtcggc cagcaggtcg tgctcttcga cgacagcgtc gccgccaaca
     2161 tcgcctacgg tcgcccggac atcccccgcg cggccatcga gcacgccgcc cgcgccgccc
     2221 atgcctggga attcatcgaa aagctgcccc agggcctgga cacccccatc ggcgagaacg
     2281 gcgcgcgcct ctccggtggc cagcgccagc gcatcgccat cgcccgcgcc ttcctcaagg
     2341 acgcgccggt gctgatcctc gacgaggcca cctcggccct cgacaccgaa tccgaacgcc
     2401 aggtccaggc cgccctcggc accctgatca agggccgcag caccctggtc atcgcccacc
     2461 gcctgtccac catcgaaggc gccgacacca tcgtcgtgat ggacaagggc cgcgtcgtgg
     2521 aacagggccg ccatgccgaa ctgctcgccc gcgacggcgc ctatgcacgc ctctaccgca
     2581 tgcagttcca ggacgaagcc cccgcctgag ccggcggggt cccagccgat tcatgaaata
     2641 atgcaggctt caaccggtac cccgcccttc ccggcgcctc tttccgtcat tcgtcccctc
     2701 tcgccatgac cccgaccctg cccgacttct cccgcggccg cgtgctcatc gtcggcgacg
     2761 tgatgctcga ccgctactgg cacggcgcca cctcgcgcat ctcgccggag gccccggtgc
     2821 cggtggtcaa ggtggacatg gacgaattcc gcgccggcgg cgccggcaac gtggccctca
     2881 acgtcgccac cctgggcggg ggcgtgagcc tggtggggct ggtgggcgac gacgaatccg
     2941 ccggactgct gcgcgcccgc ctcgaggccg gccgcgtcgc ctgccacttc gtcccggtgg
     3001 ccggcagcca caccatcacc aagctgcgcg tcatcagccg ccaccagcag ctcatccgcc
     3061 tggacttcga ggacggcttc acccgcgccg aagccgccgc cgtcaccgcc gccgtccgcg
     3121 ccggcctcga cggtgtgggg gcggtggtgc tgtccgatta cgccaagggc accctggccg
     3181 acgcccgtgc cctcgtccac gccgcccgcg aacgcggcct gcccatcgtg gtggacccca
     3241 aaggcaccga cttcgagcgc taccgcggcg ccagcgtgct cacgcccaac ctgtccgaat
     3301 tcgaggccgt ggtcggccgc tgcgccgatg aggccagcct gcgcgcccgc ggcaccgccc
     3361 tgcgcgacgc cctcgacctg gacgccctgc tggtcacccg cagcgagcgg ggcatgaccc
     3421 tgctccagcg cggccacgcc cccctcgacc tgcccaccca cgcccgcgaa gtcttcgacg
     3481 tcaccggcgc tggcgacacc gtggtggccc tgctcgccgc cgggctcgcc gccggcctgc
     3541 ccctggccga ggccaccgcc ctggccaacg tgggcgccgg cgtggtggtg ggcaagctgg
     3601 gcaccgccac cgtgtcggtg gacgagctgg ccgccgccct gcacgcgccc gccggcggcg
     3661 ctcgcggtat cgtggacgaa ccccgcctgg ccgccgccgt ggcccaggcc cgcgacgctg
     3721 gcgagcgcgt cgtgctctat cccgctgcct tcgacggcct gcgcccggcc gatctgcgcg
     3781 ccctgcaggc ggcccgccgc cagggcgacc ggctggtcgc cgcggtgctc aacgacgcag
     3841 ccgccacctg cgcactgggc gaacgcctgg ccctcgccgc cgcgctggaa gcagtggact
     3901 ggaccatcgc ctgcgacgaa tcccccgccg agctggcggc ccgcatcggg gccgagctcg
     3961 ccgtatcctg aaggagcgac catgatcatc gtgaccggcg gcgccggctt catcggcagc
     4021 aacatcgtcc agggcctcaa tgcccggggc gtcaccgaca tcctggtggt ggacgacctc
     4081 agcgacgggc gcaagtgcct caacctgtcc gacgcccaca tccacgacta cctggacaag
     4141 gacgacttcc tggcccgcct ccaggccggc gaatccttcg gcccggtgga ggcggtcttc
     4201 catcagggcg cctgctccgc caccaccgag tgggacgggc gctttgtgat gaacgtgaac
     4261 tacgcctaca gcaaggccgt gctgcgctgg tgcgtcgagc gcaaggtgcc gttctactat
     4321 gcctcgtccg cgtccgtgta cggcgaaggc ccggtcttca aggaggaccg cgcccatgaa
     4381 caccccctca acgcctacgc ctactccaag ttccagttcg actgccacct gcgcccgcaa
     4441 ctggccggct tccgcagccc cgtcgtgggc ctgcgctact tcaacgtcta tggcccgcgc
     4501 gagcagcaca agggcagcat ggccagcgtc gcctaccact tcaacaccca gctcaaggaa
     4561 ggcgaccgcc tgcgcctctt cgagggcagc gacggctacg gccccggcga gcagcgccgc
     4621 gacttcatcc acgtggacga cgtggtcgcc gtcaacctgt ggctgctcga gcaccccgag
     4681 gtctccggca tctacaactg cggcaccggc cgcgcccagc ccttcaacga cgtggcgcgg
     4741 gccaccatcg ccttccacgg ccgcggacac atcgagtaca tccccttccc cgagcacctc
     4801 aagggccgct accagagcta cacccaggcc gacatgggcg ccctgcgcgc cgccggctac
     4861 gaccgcccct tcctcagcgt cgaggaaggc gtggcccgct acatggcctg gctggccaaa
     4921 cggagctgac cgaccgtgag catggcgccc cgccgcatcc tggtggtcgg cccctcgtgg
     4981 gtcggggaca tggtcatggc ccagagcctg ttcatgaccc tcaaggcccg ccacaccctg
     5041 gtggagatcg acgtcctcgc ccccggctgg tccctgccca tcctggcccg catgccggag
     5101 gtccgccgcg gcatcgcgat gcccctcggc cacggccagt tcggtctgcg cacccgctgg
     5161 cagcttggcc gccagctcgc ccaggaccgc tacgaccagg ccatcgtcct gcccggctcc
     5221 ttcaagtccg ccctcgcccc cttcttcgcc ggcatcccgc ggcgcaccgg cttccgcggc
     5281 gagatgcgct tcggcctcct caatgacatg cgccgcctcg acaagcgtgc cctccccatg
     5341 acggtgcagc gcttcgtcgc cctcggcctg ccaccggaca cgccccagcc gccgcccacc
     5401 cccacaccgc gcctgcagcc cgatcctgcc ggctttgcgc gcctgcgcgc ccagctcgac
     5461 ctgctggccg atcgcccggg cgtatgcttc atgcccggcg ccgaatatgg ccccgccaag
     5521 cagtggccca ttccatccta cggcgaactg ggccgccgcc tggtgcggca gggcttccag
     5581 gtgtgggtgc tgggctcggc caaggacaaa cccgccggcg acgagatcgc caccctggcc
     5641 ggcgagggcg ccttcaacct gtgcggacgc accgagctcg ccgacgcggt ggacctgctc
     5701 gccaccgccc gcgccgccgt caccaacgat tccggcctga tgcacgtggc cgccgccctc
     5761 ggaacgccgc tggtggccct ctacggatcc tccacgcccc accacacgcc gcccctgtcg
     5821 gacgcggccc aggtgatcta cctcggcctg gaatgctccc cgtgcttcaa gcgcacctgc
     5881 cccctcggcc acacccgctg cctcaccggc atcggcgtgg aacgggtggc ggaggccctg
     5941 gccccggccc tgcacgccac cgcgccccga tgaacacccg cccgcctttc caggatcgcg
     6001 ccgccgacgc cgcccgctgg ctgtgcgtgg ccagcgcctt cgccgtgccc ctgccggccg
     6061 cctggctcag cttcaccacc ggcctgttcc tcatcgcctg gggcctggcc ggccgcctgc
     6121 cgctgcgctg ggcaacgatc cgccagcacc cggtcggacg cctcagcctg gtcctgctgc
     6181 tgtggatggc cgtcgccatc gcctgggcgc cggtcgagcc caaggtcggc ctggcccact
     6241 ggtggcatta ccgggaactc ctcatcgtcc cgctgatgct gtccctcttc gacgccgaca
     6301 ccgcccggcg ctggcagccg cgcgtgttca cagccttctt catcggcttt ggcatcgccc
     6361 tcgtcacctc cttcctgcgc tggaccggcg tgctgcccga catgggcttc aagggcctct
     6421 atgccggctt cggcgggcgc accggcttca gcatgctgct ggcctgggcg atcttctgcg
     6481 ccgtcgagtt tggccgcgac cagccccgct tccgcctcgc ctgggctgcg ctggccctgg
     6541 tgtgcttcgc caacctgttc ttcatcaaca ccgggcggac cgggcagatc gccttcttca
     6601 ccctggtgcc cctgagcctc taccacctgg ccggccgccg cgggctcgtc gccggcctgc
     6661 tcgccatccc ggtactggcc gccggcatgt acgccgcctc gcccgccgtg cgggcacgca
     6721 tcgccgaatc cgccgagcac atcgccgcct acgaagccgg ccatccgtac accggcgacg
     6781 gcctgcgcct ggaattcatc gccaacgcgg ccgatttcat ccgtcagcac ccggtgttcg
     6841 gcggcggtac cggcagcttc gccgccgaat acaaggtcgt cgccgagcgc gaagggatga
     6901 ccggcgagcg cgtcgctgac aacccccaca acgaatacct gatggtctgg tcccagcagg
     6961 gtctgcccgg tctggccctg ctgatcgccc tgttcctcac ccagtggcgg ctctccgccc
     7021 ggctggacga cctgccggcc cgtctcaacc gcggcctggt gctgctcatc gccggcggcg
     7081 acctggtcaa ctccctgatc ctcgacaacc tggaaggcca cctgtgggcc ctgctcaccg
     7141 tcgccctgac ggccggcatc ggcgccgacc gcgcggcccc ttcccccacc gaaagctcgc
     7201 gctgacatgg cccgccccac cctgtccgtc atcatcatcg cccgcaacga agcagcgctg
     7261 atcgccaagt gcctcgactc cgtcacctgg gcggacgaga tcgtcgtggt tgattccggc
     7321 agcaccgacg gcacgccgga gatctgccga cgctacacgg acaaagtctt cgtcaccccc
     7381 gactggcccg gtttcggccc ccagaagaat cgcgccctcg acctcgccac cagcgactgg
     7441 gtgctggccc tggacgccga cgagacgatc tccatccccc tcgccaccga gatcgccgcc
     7501 accatcgccc tcacgcccca cgacgtgtac gcggtcaagc gcctgtccag cttcctcggc
     7561 cgcccgatcc gccacggcga ctggtggccc gacgaggtgc cgcgcctgtt ccgccggggc
     7621 tgcgcccgct tctccgacga tccggtacat gagcgcctgg tcttcgaagg ccgtccggcg
     7681 cgcctcaggg cgctgatgat gcacgacagc atccgcagcc tcgaccagat gtgcgccaag
     7741 atgaaccact acaccagcgc cggcgccgag cgcctcgccc gcgccggcaa acggggcggc
     7801 ctcggcaagg cgatccgcca cggcctgtgg gccttcctgc gcgcctacct cctcaagcgc
     7861 ggcttcctcg acggccgcga aggcttcatc ctcgccgtct ccgccgccga aagcgcctac
     7921 taccgctacc tcaagcgcct gtacctgcca tgagcgcctc cgccgatctc gccgcccggc
     7981 gcttctccgt cgtcatcgtc aattacaacg gcggccagat gctcctcgac tgcgtccgct
     8041 cggtgcgcag cggcctgctc ccggccgacc ggatcatcgt ggtggacaac ggctcccgcg
     8101 acgactcgat ggcccgcctc gaagccgccg agccgggctg cgtcattctg cgcaacggct
     8161 gcaacgccgg cttcgcgcgg gccgtcaacc gcggcctggc ccaggtgcgc agcgagttcg
     8221 ccctgctcct caacaacgac gcccgcctcg cccccgacgc ctgggacgcc ctcgccgccg
     8281 ccttcgacac cctgccccgc gccgccttcc tcggcggccg cctgctctac gaggacggcc
     8341 gcctgcagaa cgccgtcgcc gcctttccgc gcctgtccgc cgaactcctg ccgcgggccc
     8401 tgctgcgcgc cctgtggccc acccgctacc gcggccgccc caccggcgac gcgccgctgc
     8461 gggtcgagag catcatcggc gccctgttcg ccgtgcgcat ggccgccgtc gccgaattcg
     8521 gccccctgga cgaggatttc ttcttcttcc tcgaagagac cgagtggtgc catcgcgccc
     8581 acacccgcgg ctgggagacc tggcacatcc ccgccgcccg cgcggtccac gcccagggcg
     8641 ccaccgccaa gaagcacaac gccttggcgc gcatcgaata ccaccgctcg cgcctgctct
     8701 acttccgcaa gaccgcgcca ggccgctacg ccctcagcta cgccatcgcg ctgaccaagg
     8761 ctgccttcaa cgccctcggc aacgccctgc tcgcggggct caccctcggc ctcgcacccc
     8821 gcgtgcgcgc caaggccggc gtggacctga agatcctcgc ctggtatctg caagggcgac
     8881 cggccgacgt gggcctgccg gacaagtgcc cgccgggccg gcccctgccc ggagcctgaa
     8941 ccgccatgaa gctgctggtc gtcaagacgt cctccctcgg cgacgtgatc cacatgctcc
     9001 ccgccgtcac cgacgccgcc cgcgcccgcc cgggcctcac ggtggactgg ctggtggagg
     9061 agagcttcgc cgccatcccg ccctggcacc cggccgtacg ccgtgtcctc cccatcgcca
     9121 tgcggcgctg gaaaaagcag tggcgccgcg cgaccacctg ggcccagctg cgcgcggccc
     9181 gcgccgccct gcaggccacc gattacgact gggtggtgga cacccagggc ctcctcaaga
     9241 gcgccctgtg gacgcgttgg gcccgtggcc cgcgctgcgg ctatgacgcc gcctccgcgc
     9301 gggaacccct cgcggcgcgc ttttacgagc gccactacgc cgtgccgcgg gagatgcacg
     9361 ccatcgaacg caaccgccgc ctcctcgccc aggccctcgg ctacaccctc gacgacccgc
     9421 gccccgacta cggcctggcc ggcctcgccg cacgcctgcc ggcaccggac gtgagcctgc
     9481 cggcgcgcta cgtggtcggc ctgcacggca ccagccgcgc cgacaaggag tggcctgtcg
     9541 aacactggaa ggccctcgcc cgcgccctcg gcggcgccgg cgccccgctg gtgatgccct
     9601 ggggcaacga ggccgagcgc cagcgtgccg aagccatcgc cgctgccgcg ccgggcgcgc
     9661 tggtgctgcc gcgcctgggc ctcgacgccc tggcggtcat cctcgaccgg gccgccgccg
     9721 tcaccggcgt ggacaccggc ctgatgcacc tggccgccgg cctcggcaag cccggcctgg
     9781 ccctgtatac cgccacccgc ccggccctga ccggcgtcgt gccggaccgc cacagccgca
     9841 gcgccctgga aaaccgggag cgcccggacg cgctgggtgc cgacgccgtg atcgccggca
     9901 tgctcgcccg ccttgcctga ggaccgccca tgagcgccat cctgcccatc ctgatgtacc
     9961 accagatcga cgccacgccg ccgcggggca cgcccctgcg cgggctcacc gtcacccccg
    10021 gcagcttcgc ccggcagatg gccctgctgc gcctgctggg ctaccgcggc gtgagcatgc
    10081 acgacctgga accctatctg cgcggcgaac ggcgcggtcg cgtggtgggt atcaccttcg
    10141 acgacggcta ccgcaacaac ctggagcacg ccctgccggt gctgcagaag cacggcttca
    10201 gcgccacctg ctacgccgtc agcgacccct tcgagggccg caacgcctgg gacgaaggca
    10261 tgggcatgcc ccagaagccc ctgtttacgc cggacgacat gcgcgcctgg gtggccggcg
    10321 gcatggagct gggcgcccac acccgccacc acgccgacct cacccggatc gacgacgcca
    10381 ccgccgagga cgagatcgcc ggctgcaagc gcgagctgga agccatcatc ggccgcgaag
    10441 tgcgccactt ctgctatccc tacggccact acggccccgc ccaccgggac atggtgcgcg
    10501 ccgccggcta ccgcagcgcc accaccgtgg aacgcagccg tgcccgcagc agcgacgacc
    10561 ccttcgagct gccgcgggtc atgatcgccc gcaccaatca cctcgccctg ttctggatga
    10621 agatcgccac cggctacgag gacagccgcg gatgacgcgc cgcctgcgca tcgccgtcct
    10681 caaccgtcac ttcggacgtc gcttcggcgg cgccgaaagc tattccgtgg ccctcgtcga
    10741 agccctcgcc gcccgccacg aactgcacgt cttcgcccag gagttcgatc accccggcat
    10801 cggcatcgcc ctgcaccggg tgccgcggcc gctggccaag ccgcgctggc tcaaccagct
    10861 gtggtacgcg gcatggacct ggcaggccac gcggcgcggc ttcgacgtgg tccattccca
    10921 cgagaacacc tggcacgggc agatccagac cgtgcacgta cgcccgctgc gggtcggcct
    10981 gttccacggg ctttccgccg ggcggcgcat cctcaagacc ctgcagatcc tcaccagccc
    11041 gcggctgctg gcctactggt ggctggaagg cagccgcttc cggccgcggc cggaccggcg
    11101 catcgtcgcc agctccacca gcgtgcgcga cgaagtcctg caggcctatc cccacgccgc
    11161 cgggatgatg tcggtgatcc cgccgggcac cctgctgccc gccggcccgg cggacccggc
    11221 ggcggcacga cgccagttga aactgccggc cgacgtgccc ctggccctct ttgccggcaa
    11281 cgactacgtc aagaagggcc tgcccgccct cctggaggcc ctcacccgcc tgcccgccga
    11341 cctgcacctg gcggtggccg gcaacccggc ccccatcgcg gctttccggg cccaagccaa
    11401 gcagctcggc gtcggggcgc gagtccattt ccttggcagc ctgcgagaca tggtgcccgc
    11461 ctataccgcc gccgacctgc tggtccatcc caccaccgag gatacctacg cgatggtggt
    11521 cctcgaagcc ctcgcccacg gtctgccggt gatcgtcagc ggacccgccc actgcggcat
    11581 cgcgacagaa ctgcgggacg gggtggacgc gctgctcgtg gacgacccgc gggacgcgga
    11641 cgccctcgcc cgcgccatcg cgcgggtgcg taccgatgtg ggcctggcgg cggccctcgg
    11701 tctggccggg cgtgcgttcg ccgccgcacg cgactgggac gcccgcgccg cggttcagga
    11761 agccctgtat ttcgaggcag cgggcaacgc gacgggctga gtccgggctc cgcgcgatcc
    11821 gactgggttc gggaaaagtg gaccggcaga ccgccgttcg gggcgccacc ttgccggacg
    11881 aacgaagggg caggtcgatg cccgccctgc cacctcacgt ggatccgccg caccgggcca
    11941 ccggggcccg gtggccacgc tgcggacggg cctcagccca gcttctgctt cagcagcgca
    12001 ttcacctggg tcgggttggc cttgcccttg ctggccttca tgcactggcc cacgagggcg
    12061 ttgaaggctt tttccttgcc ggcgcggtat tcgtccaccg atttcgggtt ggcggcgagg
    12121 acttcgtcga tgagggcctc gatggcgccg ctgtcggtga cctgcttgag gccctgggcg
    12181 tcgatgatgg cgtcggcgga ggggccttcg ccgttccaca gggcctcgaa gaccttcttg
    12241 ccgatggcgt tggagatggt gttgtcggcg atgcgctgca ccaggccggc aagctgcacc
    12301 ggcgagacgg ggctggcggc gatgtccagt tcggccttgt tgaggcgcgc cgccaggtcg
    12361 cccatcaccc agttggcgca gggcttggcc agggccgtgc cggccaccgc cacggcggcg
    12421 aggtaaaact cggcgatctc gcggctggcg gtgagggtgg tggcgtcgta ggcggagagg
    12481 ccgtattccc cctcgaagcg ggccgacagg gcggccggca gctcgggcag ttcgcccttc
    12541 acgcgctcga tccaggcgct gtcgataacc agcggcagca ggtcgggatc ggggaagtag
    12601 cggtagtcgt gggcgtcttc cttgctgcgc atggcgcggg tctcgccgga gtccgggtcg
    12661 aacagcacgg tggcctgctg gaccttgccg ccgtcttcga tcagctcgat ctggtactgc
    12721 acctcgtagt cgatggcctg ctgcaggaag cggaagctgt tgaggttctt gatctcgcgg
    12781 cgggtgccga attcggcctg gccgcgggga cgcaccgaca cgttggcgtc gcagcggaag
    12841 gagccttcct gcatgttgcc gtcgcagatg tcgatccagc gcaccagggc gtgcagggcc
    12901 ttggcgtagg ccacggcctc ggcggcggag cgcatgtcgg gctcggagac gatctccagc
    12961 agcggcgtgc cggcccggtt gaggtcgatg cccgacatgc cgtggaagtc ctcgtgcagg
    13021 ctcttgccgg cgtcttcttc caggtgggcg cgggtcaggc gcacacgctt ctcgtagtgg
    13081 ccgccctggc catccggcac cagcagggtg atggcgccgc cctggacgac gggcagctcg
    13141 tactggctga tctggtagcc cttgggcagg tccggataga agtaattctt gcgggcgaag
    13201 atgctcctcg gcgcgatggt ggcgccgatc gccagcccga agcggatggc ccgctccacg
    13261 gcgccccggt tgagcaccgg cagcacgccg ggcagggcga tgtccacggc gctggcctgg
    13321 acgttgggcg cggcaccgaa ggcggtgctg gcgccggaga agatcttgga ggcggtgttg
    13381 agctgggtgt ggatttccag cccgatgacg acttcccagt cggttctgct catggtggtt
    13441 ctcgcggtgg cgttcagtcg cactggcccg tgggccggtc gatcagcagc ggccacacat
    13501 aatcggtggc gctggcggga ttgcagtgct gcgggcagtt ggcgaaggag acggtgatgg
    13561 tctcgccctg ctcccacatg cggatgcggc agtcacgacc gccagccagt tcgaccgacg
    13621 gcagggcctg ggtctggcgg aactgggtga ggtcgtagcg gcaggtgccg cgccggggca
    13681 tgacgagttc gacggcgagg gcgcggacct tggaatcctg cacatccacg gtggccttgc
    13741 cgaagttgcc ggtctcgtcc ttgaagctgc actgggtgct caccgtgaag gtgcgcaggg
    13801 gcatgggctt gagcttgccg ctgtcggccg agggcgcggc cggcacacgg gcggccggcg
    13861 gttcgtcccg cgcgccgggc agccgcggca gctcgacgct ggcgcagccg gcgatcaggg
    13921 ctgccagcag ggacggaaag aatctatgaa acacgtctgt cattctcggt cgggacaccg
    13981 cttgaggtga agaacgcacg acaactggtt gtatcgacca gacagcgctt ccgcggagcg
    14041 gatgtcttct gcaagatcgc tcaggcgctc atacgcgaaa taaccctgaa agaaataagg
    14101 tgcccttttg ttgtcgcgga caaagaccgg aaagcaattg tcgggttgcc gggcgagcaa
    14161 acgtgccttg tgggcgacct ttgccttgtt gcccgcaaat acgatatccg gcgccttggg
    14221 attgagatca cgggcgaaac agcctgccag gattcggtga tcccgttgcg ggagatacgt
    14281 tgtcaattct ccccccacaa acgcctggat ctgagtgcgc gtatagggca cgccaaactt
    14341 cagaggtagc atcgccaccc tcacagctgc ggcgcgcggg cgtgccagtc ggtggcctgc
    14401 tggaactgat gggccacgtt gagcaggcgg gcttccgcga agtagttgcc gatgagctgc
    14461 aggccgacgg gcaggccgcc ggcgccgaaa ccggccgggt gggacaggcc gggcaggccg
    14521 gcgaggttca cggcgatggt gtagatgtcg gagaggtaca tctgcaccgg atcgtcgctc
    14581 ttctcgccga taccgaaggc caccgtgggg ctcaccggac cggcgatcac gtcgcactgg
    14641 gccagcgcgg actggaagtc ctcggcgatg aggcggcgca gcttctgggc cttgatgtag
    14701 taggcgtcgt agtagccgtg ggagagcacg taggtgccca ccaggatgcg ccgctgcact
    14761 tcgccgccga agccctgggc gcggctcttg cagtacatgt ccatcaggtc ggtgtattcg
    14821 gccgcccggt ggccgtagcg cacaccgtcg aagcggctca ggttggagct ggcctcggcg
    14881 ggggcgatca cgtagtaggc ggggatcgcc agacgcgcgt tgggcaggct gacctcgacg
    14941 gtggtggcgc cgagggcgcg gtactggtcg agggcggcgt ccacgacggc gcgcacgtcg
    15001 tcggacatgc cctcggcaaa gaattccgtg ggcaggccga tgcgcaggcc ggcgaggggc
    15061 tgggccaggt cgcggccgta gtcttcggcg gggcgttcca ggctggtgga atcccgcggg
    15121 tcgtggccgg ccatggcgga gagcagtacg gcgcagtctt cggccgtgcg ggcgaaggca
    15181 ccgccctggt cgagggacga ggcgtaggcg atcatgccgt agcggctgac cgtgccgtag
    15241 gtgggcttga tgccggtgat gccgcacagg gccgcgggct ggcggatgga gccgccggtg
    15301 tcggtgccgg tggcgatggg caccagccgc gccgccaccg ctgccgccga accaccggag
    15361 gaacctccgg gcaccgtgtc ggtgttccag gggttgcgga cggggccgaa gtgggagttc
    15421 tcgttggacg agcccatggc gaactcgtcc atgttggtct tgcccaccat caccgcgccg
    15481 gccgccttga gcaggctgac cacgtgggcg tcgtaggggc tgacgaaatt ggccagcatc
    15541 ttcgaggcgc aggtggtgag cacgccctcg gtgcagaaga cgtccttgtg agccagcggg
    15601 atgccggtga gcgggccggc gtcgcccgcg gcgaggcgcg cgtcggcggc ctgcgccgcg
    15661 gcgagagcgc cgtgccggtc cacggtgatg aaggcgttga ggcgcgggtt gagagcgtcg
    15721 atgcgggaga ggctgtcggt ggccagttcg accgcggaga cgcggcgggc gcggagggcc
    15781 gcggcgatgt ccttgaggct ggattcgatc atggtgcgtg gggcgggcta ggggcgacgc
    15841 gccggggcgg acgcgtcgcc gggagtgcgg ggcttactcg atgaccttgg gaaccaggta
    15901 gaggccgcct tcggtctgcg gcgcgatggc ctggaaggcg tcgcggcgat cggactcggt
    15961 gacggcgtcg gcgcgcaggc gctgggcgag atcctgcggg tggctcatgg gctcgacacc
    16021 gtcggtattg acggcctgca tctcttcgat caggccgaag attccattga gtttggcctg
    16081 ggtcgccgcg aggtcgtcgg tggagacttc gaggcgggcg agcttggcga tgtggcggac
    16141 ctggtcgacg gtgagggaca tggcaaatta caaagttgct taaacgacaa aggtttgtag
    16201 gttatcataa ccaattctac cggccgcgac ggcgtccggc agccccggtt tcaacccact
    16261 ccctttgctt ttcctggatt gtcctccgat gttcggttcc ctgcgctcct atttttccaa
    16321 cgacctcgcc atcgaccttg gcaccgccaa caccctgatc tacgtgcgcg gcaagggcat
    16381 cgtcctcgat gaaccgtccg tcgtggcgat ccggaccgaa ggcggaccga atgccaagaa
    16441 gaccatcctc gccgtgggca tggccgccaa gcagatgctg ggcaagacgc cgggcaacat
    16501 caccgccatc cgccccatga aggacggcgt gatcgccgac ttcaccgtca ccgagcagat
    16561 gctcaagcag t
//
LOCUS       DAOQMM010000084        16515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1347_length_16515_cov_49.941130, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000084 DAOQMM010000000
VERSION     DAOQMM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16515
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1347_length_16515_cov_49.941130"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="PLH85_12835"
     tRNA            complement(1..76)
                     /locus_tag="PLH85_12835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Lys,seq:ctt)
     gene            complement(122..197)
                     /locus_tag="PLH85_12840"
     tRNA            complement(122..197)
                     /locus_tag="PLH85_12840"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(162..164),aa:Lys,seq:ttt)
     gene            complement(264..947)
                     /gene="queC"
                     /locus_tag="PLH85_12845"
     CDS             complement(264..947)
                     /gene="queC"
                     /locus_tag="PLH85_12845"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNB65499.1"
                     /translation="MNTPRPRAIVLLSGGLDSTTCLAIARAEGFDAYALSVEYGQRHA
                     AELAAARRIAAALGAIDHRVAHVDLGQFGGSALTDPALAVPVDGVAGGIPITYVPARN
                     TIMLSIALAWAEVLGADDIYVGVNAVDYSGYPDCRPAYIDAFEKMANLATKAGVEGHR
                     LRIHAPLIDLSKADIVRRGAALGVDYGLTVSCYQAADDGAACGLCDACRLRSAGFAAA
                     GLPDPTRYR"
     gene            complement(1006..1668)
                     /gene="queE"
                     /locus_tag="PLH85_12850"
     CDS             complement(1006..1668)
                     /gene="queE"
                     /locus_tag="PLH85_12850"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNB65500.1"
                     /translation="MNTPSGHPVTLRITEIFHSLQGETSRAGLPTVFVRLTGCPLRCT
                     WCDTTYSFTGGTRRSLDDVLAEIAAYACPTVCVTGGEPLAQKGCLALLTALCDAGYSV
                     SLETSGALDIADVDPRVSRIVDLKAPGSGEEARNLLSNLDHLAPQDELKLVLASEADY
                     LWARHMLAEHRLAERCPVLLAPVQGQLPPADLAAWILRDRLPVRMQVQLHKVIWGNEP
                     GR"
     gene            complement(1721..2485)
                     /gene="ybgF"
                     /locus_tag="PLH85_12855"
     CDS             complement(1721..2485)
                     /gene="ybgF"
                     /locus_tag="PLH85_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNB65501.1"
                     /translation="MKQRLLPLLLAGLVVGLAPLRAHAGLFDDDEARARIEDMRKQLS
                     ARIDKLEASARAQLELANQIEQLKAEIARLRGENEVLANDLANANKRQKDFYVDLDNR
                     LRKVEPPVAAAADPAATPVQPAPQPPAVDPANENRDYEAALNQLRGGKYKEAATAFTA
                     FLRNYPNSSFQPSAHYWTASAQYQLKDYANAADHYGRVASQWPDDTRAPDALLGLSSA
                     QSGLGDLKAATRSLEKLVAKYPNSQAAQIARQRLKK"
     gene            complement(2485..3039)
                     /gene="pal"
                     /locus_tag="PLH85_12860"
     CDS             complement(2485..3039)
                     /gene="pal"
                     /locus_tag="PLH85_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNB65502.1"
                     /translation="MNIPRFALTLAAAGILAACSSSPKTGDEQSGAAVEDRSGAASSG
                     IDSSRVVPVTPGDAAGAGIAALKDPKSILSKRSVYFDYDSYVVKDEFRALVEAHAKFL
                     VGHPQMKMLIQGNTDERGSREYNLALGQKRSEAVKKALSLLGVPESQLESVSLGKEKP
                     SCSDATETCWSANRRGDMLYSGEF"
     gene            complement(3114..4403)
                     /gene="tolB"
                     /locus_tag="PLH85_12865"
     CDS             complement(3114..4403)
                     /gene="tolB"
                     /locus_tag="PLH85_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNB65503.1"
                     /translation="MKATFLTLLRGLLPGCALALAAATAQAQLAIEITGAGANRLPVA
                     IAAFEGEAALPRPVTDVVKADLERSGLFKLIELGPTPQGYDAQVNLPGVRSRGADALA
                     VGSVVTTGNGRYEVRVRLYDTPKQAALGVQAMTMSPAQYRTTGHRIADFIYEKLTGEP
                     GVFSTRIAYVLKSGPRYELQVADADGSNAQTALASKEPIISPAWSPDGTRLAYVSFES
                     KKPVVYVHSLATGQRNVAANFKGSNSAPAWSPDGRRLAVVLTKDGQSQLYAVNADGSG
                     VQRLASSAGIDTEPAWSPDGAWIYFTSDRGGSPQIYRIPSSGGDAQRVTFEGSYNVSP
                     RPAPDGSNLVYVSRNGGRFQVTMMDLNSRQTQILTDSSKDESPSFAPNGRMILYATEI
                     GGRGVLAAVSSDGRVKQRLSVQSGDVREPAWGPQPRN"
     gene            complement(4462..5427)
                     /gene="tolA"
                     /locus_tag="PLH85_12870"
     CDS             complement(4462..5427)
                     /gene="tolA"
                     /locus_tag="PLH85_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNB65504.1"
                     /translation="MNPALPGNERGKWSSAALAAGVHVLLGLFLFYGVRWQNRLPEPV
                     EVELVSAPPVTAPVTPPPPPPAPAEERPAPPPPPRPEPEPEPAPPPRKQPDIAIKAPE
                     PRKLPPKPVKEVVKPEPKPEPKKPEPKPEPRKPEPKPEPKKPDPKPEPKPEPKPDPKA
                     EAKARAEQARLDKAKADKDAKAASDRAREQEDRRMRELLAHDSENAKQSAQIQEELAR
                     IAGAKASAARNKAIEAYIEKIRAKVKGNIVLPPGVAGDPFALFYVDQLPDGSVIEVRL
                     KRSTGTPQLDSAIERAIQKSSPLPRPDKPELFNRTLELKFYPLRD"
     gene            complement(5424..5837)
                     /gene="tolR"
                     /locus_tag="PLH85_12875"
     CDS             complement(5424..5837)
                     /gene="tolR"
                     /locus_tag="PLH85_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNB65505.1"
                     /translation="MRQRRLMNQINVVPYIDVMLVLLVIFMVTAPMIQSGSVNLPSVD
                     KVPAPPSDAMIVTVKPDGELTLAQTSQSQPRSYNRRDLIGALQDATSANPGQPVVIAA
                     DKDVKYERVMDILNDLRNAGVNKVGLQTQIGKSAR"
     gene            complement(5854..6528)
                     /gene="tolQ"
                     /locus_tag="PLH85_12880"
     CDS             complement(5854..6528)
                     /gene="tolQ"
                     /locus_tag="PLH85_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNB65506.1"
                     /translation="MNVTEDLSILSLIANASLLVKLVMALLGTVSFMSWYWIFRKWFQ
                     IRDAKKKTDLFERDFWSGGDLNALYQSAATSRHEAAGMERIFESGYREFAKLRGKNLD
                     HGAIMDGAKRAMRATYQREVDDLEAHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLSN
                     VGSATLAHVAPGIAEALVATAIGLFAAIPAVVAYNRFAHDIDRIAIRFESFTEEFLNI
                     LQRQLR"
     gene            complement(6533..6988)
                     /gene="ybgC"
                     /locus_tag="PLH85_12885"
     CDS             complement(6533..6988)
                     /gene="ybgC"
                     /locus_tag="PLH85_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNB65507.1"
                     /translation="MHAQTDPARPALSEQSVRPSFTLPVRVYYEDTDAGGVVYYANYL
                     KFCERARTDWLRSCGIGQHRLASEDGVLFVVRSVRADYRQPAVLDDDLAVVSTLAKLG
                     HASLTFHQKVLRDGVELFDAQVVIACIDRDARRAIPIPPAVRAQFATLV"
     gene            7279..7707
                     /gene="dksA"
                     /locus_tag="PLH85_12890"
     CDS             7279..7707
                     /gene="dksA"
                     /locus_tag="PLH85_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNB65508.1"
                     /translation="MVATTKDKILTEAEILAAPEEEYMNPAQLEFFRDRLSRMRQELL
                     DNAASTGASLRENEIVADPADRATVEEEHALELRVRDRERKLLKKIDEALNRIAEGEY
                     GWCEETGDPIGIGRLLARPTATLSIDAQERRERTQKLYGE"
     gene            7762..8817
                     /locus_tag="PLH85_12895"
     CDS             7762..8817
                     /locus_tag="PLH85_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB65509.1"
                     /translation="MRAAHRIPVTLLTGFLGAGKTTLLNHLLRRPDMAGTVVVINEFG
                     SIPLDHLLVTHLDEQVVLLESGCLCCTVRGDLSRCLRELFMRRLRRELPALDRVIVET
                     TGLADPAPVLFTLMNDFFVAERYRLDGVVTAVDGVFGADQLAARPEAVKQAAMADRLV
                     ITKVDQATPAQLDAVDARLARLNPGAPRLRSVGGAVDPADLLDCGLWNPVTHSADVAR
                     WLGDGAVAAQRDPDYRPLTAKPSAPAGQHDAAVRAFALRLPAPVAWGDFSAALDALQS
                     LWGERLLRVKGLVHVAGEVSPRVIQCVHHLRYPDAALPAWPQGARETRLVFIVRELDP
                     AVVERAFACFCVPELVA"
     gene            8814..9179
                     /locus_tag="PLH85_12900"
     CDS             8814..9179
                     /locus_tag="PLH85_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65510.1"
                     /translation="MRRMPFRGRPSALFRGTLALLLVCAQLLGAMHAIGHFEAAERPL
                     RVDAQAAPASGGQPATERHERCLLCLAAADLGAALPVTPSLPGPEAPVSALPPDAGTL
                     PAFLRPPTPSSRDPPVSPV"
     gene            9260..10678
                     /locus_tag="PLH85_12905"
     CDS             9260..10678
                     /locus_tag="PLH85_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65511.1"
                     /translation="MSIVRPGALCVALAAALPAVASAADSAADLKQLRDELRALRDTY
                     QQRIDALEKRLADSEARAARAETTATAAARSAEQAEQKAAALPDVAIAPATPPSGEGA
                     FNPAVSLILDSKFTRFQQDPAKYRLDGFIPSGGEVGPPRKGFSLGESELAVSANVDHL
                     FRGNARFSLADDGAGGTVEVEEAHIETLALPEGLKLKAGRFLSGVGYLNVQHPHEWDF
                     TDAPLAYKAFLGPRLQNDGVQLRWVAPTDLFVELGGELASGQKYPGAERAANGAGLWT
                     AFAHVGGDVGASSAWQVGLSHVHANPRARTFEDGGVSNAFSGRSRLWIADFVYKWAPD
                     GNPTVNNLKLQGEFFRRSEDGDLTYDLAGAGQASAASFRQSGGYLQAVYQFMPHWRAG
                     VRGDWLRAGSIDLGSLSTTELPGLVVYNPSRQSAMVDWSPSEFSRLRLQLARDTSRAG
                     ETDHQLWLQYIMSLGAHGAHKF"
     gene            10690..11598
                     /locus_tag="PLH85_12910"
     CDS             10690..11598
                     /locus_tag="PLH85_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNB65512.1"
                     /translation="MLRTLLLRLVALVLFTSAGAASAALNVFATVPEWAALAKEIGGD
                     QVTVFAATTALQDPHRIEARPSLIARARSAGLVVATGAELEIGWLPMVLRESGNAAVQ
                     PGQPGYFEAATVVRMLEVPTRLDRADGDVHPAGNPHLQLDPRNILRVGEALAARLAQV
                     DPAHAEVYKANLARFGERWRAAQARWEQAAAPLRGVPVWVQHRAFPYLSAWLGLKEVG
                     ALEAKPGVEPGSAQLADVLARQKAMPARMVLRPAYARATASDWLAERAGIPAVVLPFT
                     VGGSAEAQDLFGLFDDTVRRLLAALK"
     gene            11609..12190
                     /locus_tag="PLH85_12915"
     CDS             11609..12190
                     /locus_tag="PLH85_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB65513.1"
                     /translation="MSGHLLRVSGLVAGYREPVTAPVSFTLKRGEILGIAGPNGAGKS
                     TLLKALWGGVRVFAGAVEKAPGLSISHQAQGFDDLRGVPLSGRELLRLTGAPPDGLPP
                     WVQERLDRRLDRLSGGQFQFLRLWACLAAPADLILLDEPSNNLDTAGVAYLADWLGRA
                     HGDQGIVIVSHDAALMAQVCTRILHVDAVEVAA"
     gene            12187..12978
                     /locus_tag="PLH85_12920"
     CDS             12187..12978
                     /locus_tag="PLH85_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNB65514.1"
                     /translation="MSLEFLVDPLFRLPFLTGLALAGLLPVLGMYLRLRNEWLAALAF
                     AQMASGGALVAMVADAPMLLGGAVAALLAALVKGFAARAGASAYALMMLLGWGGGVLL
                     VANHPLAEQAGHALFDGQLYFTGGAHLLAALIAVIVAMPVLAWLSRPLLLGRFFPEFF
                     RARGLDERPRLVLFDLIAGLSVALATVSLGVMAAFALVFVPPMVVFPRAPGWRVGLLV
                     AAVVGMGGYVTAFALALVFDQPFGPVCAILLVLLSGLVGLIGRQS"
     gene            13078..14274
                     /locus_tag="PLH85_12925"
     CDS             13078..14274
                     /locus_tag="PLH85_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB65515.1"
                     /translation="MVLPFFGKKPPPAGSSTPRSSARSPSGSSAAAARPGLPQPDRNE
                     TSSLDFTIAGGDIKRVLAQCADRVHVEEGVDEMSPPAEEAAILYANGAWNEARSVLEH
                     ALQTTGSSDARLGVWVMLLDLLRLTGQREQFDALSLAFATEFERSPPAWQDLSSQSAR
                     PMASKTPLVNLSGALSGQAAAQFAQLIQVGMRSGALRIDLTRLRSIDQAGAELLLRAV
                     RGLRKARIRLSLVGAPHLVNLVSGQVVRGRADNKTQWLLLLEALQYTDQQERFEELAV
                     DYAMTFEESPPSWEPPMVTESQTEAVEAVGMNDIFVLEGEVVGPNAEALRKLAAFAND
                     RNKIEVDCGRLRRMDFVSAGNLFNIVSQLHSKGRLVVLKSVNSMVAALMQVMSLHEVA
                     RIELRG"
     gene            14358..14897
                     /gene="hslV"
                     /locus_tag="PLH85_12930"
     CDS             14358..14897
                     /gene="hslV"
                     /locus_tag="PLH85_12930"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNB65516.1"
                     /translation="MEQYHGTTILSVRRGRHVALGGDGQVTLGNIVIKASARKVRRLY
                     QDRVLIGFAGGTADAFTLFERFEAKLEKHQGHLLRSAVELAKDWRTDRALRRLEAMLA
                     VADLDNSLIITGNGDVLEPEQGIVAIGSGGAYAQAAARALVENTELAPEDVVKKALSI
                     AGDLCIYTNQSHTIEVLKP"
     gene            14908..16245
                     /gene="hslU"
                     /locus_tag="PLH85_12935"
     CDS             14908..16245
                     /gene="hslU"
                     /locus_tag="PLH85_12935"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNB65517.1"
                     /translation="MTQMTPPEIVSELDKHIVGQSRAKRAVAVALRNRWRRSQVAEPL
                     KSEITPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVDTIIRD
                     LVEIAIKSHREEAMKKVRDRAQDAAEDRILDVLLPPARPTMGFGNEAPSAEENATRQK
                     FRKKLREGDLDDKEVDIEVSAPGMTAEILAPPGMEELTGQLQSMFQNLGGGKKKSRKL
                     KIREALKLLTDEEAGRLVNDEDVKLEALRRVEQNGIVFLDEIDKIATRSDSHGADVSR
                     QGVQRDLLPLVEGTTISTKYGMVKTDHILFIASGAFHLSRPSDLIPELQGRFPIRVEL
                     ESLSVEDFERILTQTDACLTRQYEALLATDGVSLSFAPDGIRRLAEIAFQVNEKTENI
                     GARRLYTVMEKLLEELAFEAGRAGNQTVNIDAAFVDGRLETLAQREDLARYVL"
     gene            16380..>16515
                     /locus_tag="PLH85_12940"
     CDS             16380..>16515
                     /locus_tag="PLH85_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB65518.1"
                     /translation="MLLRIVSILFPLFALTALGYLVGRRQRPDLSHANKLNMDVFVPA
                     L"
BASE COUNT         2375 a         5386 c         6017 g         2737 t
ORIGIN      
        1 tggtgggttg tgagtggctc gaacactcga cctacggatt aagagtccgc tgctctacca
       61 actgagctaa caacccattg tgtgctgcga gtcgcgcatt ctaacccaat ttttggatca
      121 gtggtaggtc gtgcaggatt cgaacctgcg accaacggat taaaagtccg ctgctctacc
      181 aactgagcta acgacccgcg cagagagacg cggattatag cagcggaaac atgatcgtca
      241 agcccgaacg gtcggcgggg cgatcagcgg tagcgggtcg ggtcgggcag gccggcggcc
      301 gcgaagccgg cgctgcgcag gcggcaggcg tcgcacaggc cgcaggccgc gccgtcgtca
      361 gcagcctgat agcaggacac ggtgagtccg tagtccacgc ccagtgccgc gccgcggcgc
      421 acgatgtcgg ccttcgaaag gtcgatcagg ggcgcgtgga tgcgcaggcg atggccttcg
      481 acgccggcct tggtggcgag attggccatt ttctcgaagg cgtcgatgta ggccgggcgg
      541 cagtccgggt agcccgagta gtccaccgcg ttgacgccca cgtagatgtc gtcggcgccg
      601 agtacttcgg cccaggccag ggcgatggac agcatgatgg tgttacgcgc cggcacgtag
      661 gtgatgggga tgcccccggc gacgccgtcc acgggcaccg cgagggccgg gtccgtgagg
      721 gcggagccgc cgaactggcc cagatccacg tgggcgaccc ggtggtcgat ggcgccgagc
      781 gcggcggcga tgcggcgcgc ggcggccagt tcggcggcat ggcgctgacc gtattcgacg
      841 gacagggcat aggcgtcgaa gccttcggcg cgggcgatcg caaggcaggt ggtggagtcg
      901 agtccgccgg acagcaggac gatggcgcgg gggcgtgggg tgttcatggg agctcctggc
      961 gaggcggtca gggtaacgcg aacgaccaat cgggaacagg gggcgtcagc ggcccggttc
     1021 gttgccccag atgaccttgt gaagctggac ctgcatgcgt acgggcaggc ggtcccgcag
     1081 gatccaggca gccagatcgg ccgggggaag ctggccctgg accggggcga ggaggaccgg
     1141 gcagcgttcg gccagccggt gttcggcgag catgtggcgg gcccagaggt aatcggcctc
     1201 gctggcgagg accagcttga gctcgtcctg cggcgcgagg tggtccaggt tgctcagcag
     1261 gttgcgcgct tcttcaccgg agcccggggc cttgaggtcg acgatgcgtg agacgcgcgg
     1321 atccacgtcg gcgatgtcga gggcgccgct cgtctcgagg gagacggaat agcctgcgtc
     1381 gcacagggcg gtgaggagcg cgagacaacc cttctgggcg aggggctcgc cgcccgtgac
     1441 gcacacggtg gggcacgcgt aggcggcgat ttcggccagg acgtcgtcga ggctgcggcg
     1501 cgtgccgccg gtgaatgagt aggtcgtgtc gcaccaggtg cagcgcaggg ggcagccggt
     1561 gaggcgtacg aagacggtgg gcaggccggc gcgggaggtc tcgccctgca gggagtggaa
     1621 gatctcggtg atgcgcaggg tgacggggtg gccggagggg gtgttcatgg gcgatgcggg
     1681 ggcgggtgcg ccccggcccg atggggcacg gggcgtcggc ttacttcttg aggcgctggc
     1741 gggcgatctg ggccgcctgg ctgttggggt acttggcgac gagcttttcc aggctgcggg
     1801 tggcggcctt gaggtcgcca aggccggact gggcactgga gagaccgagc agggcgtccg
     1861 gtgcgcgggt gtcgtcgggc cactggctgg cgacgcgacc gtagtggtcg gcggcgttgg
     1921 cgtagtcctt gagctggtac tgggcgctgg cggtccagta atgggcgctg ggctggaagc
     1981 tgctgttcgg gtaattgcgc aggaaggcgg tgaacgctgt ggcggcttcc ttgtacttgc
     2041 cgccgcgcag ctggttgagg gccgcttcgt agtcgcggtt ctcgttggcc ggatcgactg
     2101 cagggggctg cggcgccggc tgcacaggcg tcgcagccgg gtcggccgcg gcggcgacgg
     2161 gcggctcgac cttgcgcagg cggttgtcca ggtcgacgta gaagtccttc tgccgcttgt
     2221 tggcgttggc cagatcgttg gccagaactt cgttctcgcc gcgcaggcgg gcgatctcgg
     2281 ccttgagctg ttcgatctgg ttggccagtt caagctgggc gcgggcgctg gcttcgagct
     2341 tgtcgatgcg ggcgctgagc tgcttgcgca tgtcctcgat gcgggcgcgg gcctcgtcgt
     2401 cgtcgaagag ccccgcgtgg gcccgcagcg gcgccagccc gaccaccagc ccggccagca
     2461 gaaggggcag aaggcgctgc ttcatcagaa ctcacccgaa tagagcatgt cgccgcggcg
     2521 gttggcagac cagcaggttt cggtggcgtc gctgcagctg ggcttttcct tgccgaggct
     2581 gacggattcg agctggcttt cgggcacgcc aagcagggac agggccttct tgaccgcttc
     2641 ggagcgcttc tggccgaggg cgaggttgta ctcgcggctg ccgcgttcgt cggtgttgcc
     2701 ctggatgagc atcttcatct gcggatggcc gaccaggaac ttggcgtggg cttccacgag
     2761 ggcgcggaat tcgtccttca ccacgtagct gtcgtagtcg aagtagacgc tgcgcttgga
     2821 gaggatgctt ttggggtcct tgagggcggc gatgccggcg ccggcggcgt cacccggggt
     2881 gacggggacc acgcggctgc tgtcgatgcc ggagctggcg gcgccggagc ggtcttcgac
     2941 ggcggcaccg gactgctcgt caccggtctt ggggcttgag gagcaggcgg ccaggatgcc
     3001 ggcggcggcg agggtgaggg cgaagcgggg gatgttcatg ggggactccg tgagcggtcg
     3061 caaggggcgg gttaatggaa tgaccagctt atgggcgcgt ggtttcagat cggtcagttg
     3121 cgaggttgcg ggccccaggc gggttcgcgt acgtcgccgg attgtaccga cagtcgctgc
     3181 ttgacacgac cgtcggacga cacggcggcg agcacgccgc gaccgccgat ctcggtggcg
     3241 tagaggatca tgcggccgtt gggggcgaag ctgggggatt cgtccttgga cgagtcggtg
     3301 aggatctggg tctggcggga attgagatcc atcatcgtga cctggaagcg gccgccattg
     3361 cgggacacgt agacgagatt gctgccgtcg ggggcggggc gcgggctcac gttgtagctg
     3421 ccctcgaagg tgacgcgctg ggcgtcgccg ccgctgctgg ggatgcggta gatctgcggg
     3481 ctgccgccgc ggtcggaggt gaagtagatc cacgcgccgt cgggcgacca ggccggttcg
     3541 gtgtcgatgc cggcggagct ggcgaggcgc tgcacgccgg agccgtccgc attgacggcg
     3601 tagagctggg actggccgtc cttggtgagg accacagcca ggcggcggcc gtccggcgac
     3661 caggcgggcg ccgagttgga gcccttgaag ttggcggcca cgttgcgctg accggtggcg
     3721 agggaatgca catacaccac gggcttcttc gattcgaagg acacgtaggc gaggcgcgtg
     3781 ccgtcgggcg accaggccgg cgagatgatc ggctccttgg aggccagcgc ggtctgggcg
     3841 ttgctgccgt cggcgtcggc cacctgcaat tcgtagcggg ggccggattt gaggacgtag
     3901 gcgatgcggg tggagaacac gcccggttca ccggtgagct tctcgtagat gaagtcggca
     3961 atgcggtggc cggtggtgcg gtactgggcg ggcgacatgg tcattgcctg cacgccgagg
     4021 gcggcctgct tgggggtgtc gtagaggcgc acgcggactt cgtagcggcc gttgccggtg
     4081 gtcaccacgc tgcccaccgc gagggcatcg gcaccgcggc tgcgcacgcc gggcaggttg
     4141 acctgggcgt cgtagccctg gggtgtgggg ccgagttcga tgagtttgaa caggccgctg
     4201 cgctccaggt cggccttgac cacgtcggtg acggggcgcg gcagggccgc ctcgccttcg
     4261 aaggcggcga tggcgacggg caggcggttg gcgcctgcgc cggtgatctc gatggcgagc
     4321 tgggcctggg ccgttgcggc ggccagcgcc agtgcgcagc cggggagcag gccgcgcagg
     4381 agggtcagga aggtcgcttt catgggtgga tggacgtcat cagtgtgaat ggagcgggac
     4441 ggggccgttc gggcggcctt ctcagtccct caggggatag aacttgagtt ccagcgtgcg
     4501 gttgaagagc tccggcttgt ccggtcgagg cagcgggctg gacttctgga tggcccgctc
     4561 gatggccgag tcgagctgcg gcgtgccggt ggagcgcttg aggcggactt cgatcacgga
     4621 accgtcggga agctgatcca cgtaaaacag ggcaaaggga tcgccggcca cccccggcgg
     4681 cagcacgatg tttcccttga ccttggcgcg gatcttctcg atgtaggcct cgatggcctt
     4741 gttgcgcgcg gcactggcct tggcaccggc gatgcgtgcc agctcttcct ggatctgcgc
     4801 ggactgcttg gcattctcgc tgtcatgggc gagcaattcg cgcatgcgcc gatcctcttg
     4861 ctcgcgggcg cgatcggagg ccgccttggc gtccttgtcg gccttggcct tgtccagtct
     4921 ggcctgctcg gcgcgggcct ttgcttcggc cttgggatcc ggtttgggtt cgggcttcgg
     4981 ctcgggcttg gggtccggct tcttcggttc gggcttgggc tcgggctttc tgggctcagg
     5041 cttgggttcg ggctttttcg gctcgggttt cggctccggc ttgacgacct ccttcaccgg
     5101 cttgggcggg agtttcctgg gctcgggcgc cttgatggcg atgtcgggct gcttgcgtgg
     5161 cggcggcgcg ggctcgggtt ccggttccgg ccggggcggc ggaggcggcg cagggcgctc
     5221 ctcggcgggg gccggcggcg gtggcggcgg ggtgaccggt gccgtgacgg gcggggcgct
     5281 caccagctcc acctcgaccg gctcgggcag gcggttctgc cagcgtacgc catagaacag
     5341 gaacaggccg agcagtacgt ggacccccgc cgcgagggcg gcggaggacc acttgccccg
     5401 ttcgttgccg ggcagcgcag ggttcatcgg gccgacttgc cgatctgcgt ctgcaggccg
     5461 accttgttga cgcccgcgtt gcgcaggtcg ttgaggatgt ccatcacgcg ctcgtacttc
     5521 acgtccttgt cggcggcgat caccacgggc tggcccgggt tggccgaggt ggcatcctgc
     5581 agggcgccga tcaggtcgcg gcggttgtag ctgcgcggct gggactggct ggtctgggcg
     5641 agggtgagtt cgccgtcagg cttgacggtg acgatcatgg cgtcgctggg cggtgcgggc
     5701 accttgtcca cgctgggcag gttgaccgag ccggactgga tcatcggcgc tgtgaccatg
     5761 aagatgacga gcagcaccag catcacgtcg atgtagggga cgacgttgat ctggttcatc
     5821 aggcggcgtt gacgcatggc gacttcgctc cggtcagcgc agctggcgct ggaggatgtt
     5881 caggaactct tcggtaaagc tctcgaagcg gatcgcgatg cggtcgatgt catgggcgaa
     5941 gcggttgtag gcgaccaccg cggggatcgc cgcgaaaagg ccgatggcgg tcgccaccag
     6001 ggcttcggcg atgcccggcg ccacgtgggc gagggtcgcg gagcccacgt tggagagccc
     6061 gcggaaggcg ttcatgatgc cccacaccgt gccgaacagg ccgacgtagg gcgacaccga
     6121 accgaccgag gcgaggaagg cgaggtgggc ttccaggtcg tccacctcgc gctggtaggt
     6181 ggcgcgcatg gcccgcttgg cgccgtccat gatggcgccg tggtccagat tcttgccgcg
     6241 cagcttggcg aactcccggt agccggattc gaagatgcgc tccatgccgg cggcctcgtg
     6301 gcggctggtg gcggcggact ggtacagggc gttgaggtcg ccgccgctcc agaagtcgcg
     6361 ctcgaagagg tcggtcttct tcttggcgtc gcggatctgg aaccacttgc ggaagatcca
     6421 gtaccaggac atgaaggaca cggtgccgag cagcgccatg acgagcttga ccagcaggct
     6481 ggcgttggcg atcagggaca ggatggacag gtcttcggtg acgttcatgg ggtcaaacca
     6541 gggtggcgaa ctgtgcgcgc acagcgggtg gaatcggaat ggcgcggcgg gcatcgcggt
     6601 cgatgcaggc gatgacgacc tgggcgtcga acagctcgac gccgtcgcgc aggactttct
     6661 ggtggaaggt caggctggca tggccgagct tggcgagggt gctgacgacg gccaggtcgt
     6721 catccagcac ggcgggctgg cggtagtcgg cgcggacgga gcgtaccacg aagagcaccc
     6781 cgtcctcgct ggccaggcgg tgctggccga tgccgcagct gcgcagccag tcggtgcgcg
     6841 cccgttcgca gaacttgagg tagttggcgt aatagaccac cccgccggca tcggtgtctt
     6901 catagtagac acgcacgggg agggtgaagg aggggcggac cgactgctcg gaaagcgcgg
     6961 ggcgggcagg gtcggtctgg gcatgcattg tgacatttta ccgtagctgg ggacgtgtac
     7021 cggttctggc gcgtgccggc gctgtttcaa aatgtagggg cggaacgggg gcggtgattg
     7081 tatttgccgc gttcctgtgc ctagaatccc gccctgtgcc cgggccggcg tcggacagac
     7141 gcggccggat gcaccggcac ccggatgcgt cgggggtgaa gccgttgccc agtacgttgc
     7201 tggaaacgcg tgacaggctg cgtcccggag gacgcggctg gcgtgggatc cgcgtgtcga
     7261 atcaaggagg aacgagacat ggttgctacc accaaggaca aaattctgac cgaagccgaa
     7321 attctggcgg cgcccgaaga agagtacatg aacccggcgc agctggaatt cttccgtgac
     7381 cgcctgagcc gcatgcgcca ggagctgctg gacaatgcgg ccagcacggg cgcctccctg
     7441 cgcgagaacg agatcgtcgc cgatccggct gaccgggcca ccgttgaaga agagcacgcc
     7501 ctcgagctgc gtgtccgcga ccgggagcgc aagctgctga agaagatcga cgaggccctc
     7561 aaccgcatcg ccgaaggcga atacggctgg tgcgaggaga ccggcgaccc catcggcatc
     7621 ggccgcctgc tcgcccgtcc tacggccacc ctgtccatcg acgcccagga gcgccgggaa
     7681 cggacccaga agctgtacgg ggaataagcc ggcgccccgc cgtcgcgtgg tgacggaccc
     7741 gccccgcgga tgaccgaccc gatgcgcgcc gcacaccgaa tccccgtcac cctcctcacc
     7801 ggcttcctcg gcgccggcaa gaccaccctt ctcaatcatc tgctgcgccg gcccgacatg
     7861 gccggcacgg tggtggtcat caacgagttc ggcagcatcc ccctcgacca cctgctggtg
     7921 actcacctgg acgagcaggt cgtgctcctc gaatccggct gcctgtgctg caccgtgcgg
     7981 ggcgacctgt cccgctgcct gcgggaactc ttcatgcggc gcctgcgtcg cgaactccct
     8041 gccctcgacc gggtgatcgt cgaaaccacc ggtctggccg atccggcgcc ggtgctgttc
     8101 accctgatga atgatttctt cgtcgccgag cgctaccgcc tcgatggcgt ggtgacggcc
     8161 gtggatggcg tcttcggcgc cgaccagctg gccgcgcggc cggaggccgt caagcaggcg
     8221 gcgatggccg accggctcgt catcaccaag gtggaccagg ccacgccggc ccagcttgac
     8281 gcggtcgacg cccgcctggc gcgcctcaac cccggtgcgc cgcgcctgcg cagcgtgggt
     8341 ggcgcggtcg atccggccga tctgctcgac tgtggcctgt ggaatcccgt cacgcactcg
     8401 gccgatgtgg cgcgctggct gggggacggg gcggtcgccg cccagcgcga tccggattac
     8461 cgcccgctga ccgcgaagcc ctctgcgccc gccggacagc acgatgcggc ggtccgtgcc
     8521 tttgcgctcc gcctgcccgc gcctgtcgcg tggggcgatt tctcggcggc cctggatgcc
     8581 ctgcagagcc tgtggggcga gcgcctgctg cgggtgaagg gcctcgtgca cgtggccggc
     8641 gaggtgagtc cgcgggtgat ccagtgtgtc catcacctgc gctatccgga tgcggccctg
     8701 ccggcgtggc cgcagggtgc gcgggagacc cgcctggtgt tcatcgtgcg ggagctcgac
     8761 ccggccgtgg tggagcgcgc cttcgcctgt ttctgtgtgc cggagctcgt cgcgtgaggc
     8821 gcatgccctt ccgcggccgg ccctcggccc tgttccgcgg cacgctggcg ctgttgctgg
     8881 tgtgcgccca gctgctcggg gcgatgcatg ccatcggcca cttcgaggca gcggagcgtc
     8941 cgttgcgcgt cgatgcgcag gctgccccgg cgagcggtgg ccagccggcg accgaacgcc
     9001 acgagcgctg cctgctgtgt ctggctgccg ccgatctggg cgcggccctg ccggtcaccc
     9061 cgtctctgcc ggggccggaa gcacccgttt cggcccttcc gcccgatgct ggcaccctgc
     9121 cggccttcct ccgcccgccg acgccgtcga gccgcgatcc gcccgtttct cccgtctgac
     9181 cccgttcccc ccgtgaccgg ccggcgctct ggcgccgacc ggctgtgagc ttgacggacc
     9241 gatccgagga gatccgacca tgtccatcgt acgaccgggc gcgctgtgcg tcgccctggc
     9301 ggcggccttg cccgccgtcg cgtccgcggc cgacagcgcc gccgacctga agcagctgcg
     9361 cgacgaactg cgtgccctgc gcgacaccta ccagcaacgc atcgacgccc tcgaaaagcg
     9421 ccttgccgac agcgaggccc gcgcagcgcg cgccgaaacc accgccaccg cggcggcccg
     9481 cagtgcggag caggccgagc agaaggccgc ggccctgcct gacgtggcca tcgcgcccgc
     9541 caccccgccc tccggcgaag gggccttcaa ccccgccgtg tcgctgattc tcgacagcaa
     9601 gttcacgcgc ttccagcagg atccggcgaa gtaccgcctc gacggcttca tcccctcggg
     9661 cggcgaagtg gggccgccgc gaaagggctt cagcctgggc gaatccgagc tggcggtctc
     9721 cgccaacgtg gatcatctgt tccgcggcaa cgcccgcttc tccctggccg atgacggcgc
     9781 cggcgggacg gtcgaggtgg aagaggcaca catcgagacc ctggccctgc cggagggcct
     9841 caagctcaag gccgggcgct tcctgtccgg cgtgggctac ctcaacgtgc agcatcccca
     9901 cgagtgggac ttcaccgacg cgcccctcgc ctacaaggcc ttcctcgggc cgcgtctgca
     9961 gaacgacggg gtgcagctgc gctgggtggc gccgacggac ctgtttgtcg aactgggcgg
    10021 tgagctggcc agcgggcaaa aatatccggg cgccgagcgg gccgccaacg gggccgggct
    10081 gtggacggcc ttcgcccatg tgggcggcga tgtgggtgcc tcgtcggcgt ggcaggtcgg
    10141 gctgtcccac gtccatgcca atccccgcgc gcggaccttc gaggacggcg gtgtaagcaa
    10201 cgccttctcg ggccgcagcc ggctgtggat tgccgacttc gtctacaagt gggcgccgga
    10261 tggcaatccg acggtcaaca acctcaagct gcagggtgaa ttcttccggc gcagcgagga
    10321 cggggatctc acctatgacc tggcgggtgc cgggcaggca tcggcggcaa gtttccgcca
    10381 gtccggcggt tatcttcagg cggtctacca gttcatgccc cactggcgcg ccggggtccg
    10441 gggtgactgg ctgcgggccg gcagcatcga cctggggagc ctgagcacga cggagctccc
    10501 cggcctggtg gtctacaacc cgagccgcca gtcggcgatg gtggattggt cgccgtcgga
    10561 gttctcgcgc ctgcgcctgc agctggcccg cgacacctcc cgcgccggtg agaccgatca
    10621 ccagctctgg ctgcaataca tcatgagcct cggcgcccac ggcgcccaca agttctgagg
    10681 aacacgacca tgctccgtac cctgctgttg cgcctcgtgg cgctcgtgct gttcacgtcc
    10741 gccggcgctg cgtcggcggc cctcaacgtc ttcgccaccg tgcccgaatg ggccgccctg
    10801 gccaaggaga tcggcggcga tcaggtgacc gtcttcgccg ccaccacggc cctgcaggac
    10861 ccgcaccgca tcgaggcacg cccctcgctg atcgcccgcg cccgcagcgc cgggctggtg
    10921 gtggccaccg gcgccgagct ggagatcggc tggctgccca tggtcttgcg ggagtccggc
    10981 aacgccgcgg tgcagcccgg ccagcccggc tacttcgaag cggcgacggt ggtgcgcatg
    11041 ctggaagtgc cgacccgcct ggaccgggct gacggggacg tgcatcccgc cggcaatccc
    11101 cacctccagc tcgatccgcg caacatcctg cgggtgggcg aggccctggc ggcgcggctg
    11161 gcccaggtgg atccggccca cgcggaggtc tacaaggcca atctggcccg cttcggcgag
    11221 cgctggcggg ccgcccaggc gcgctgggag caggcggcgg cgcccctgcg cggtgtaccg
    11281 gtgtgggtcc agcaccgggc ctttccctac ctgtccgcgt ggcttggcct gaaggaagtc
    11341 ggcgccctgg aggccaagcc tggcgtggag ccgggcagcg cccagcttgc cgatgtgctc
    11401 gcccgccaga aggcgatgcc ggcgcgcatg gtgctgcgcc cggcctatgc ccgtgccact
    11461 gcgtccgact ggctggcgga gcgggccggc attccggcgg tggtgctgcc cttcaccgtg
    11521 ggcggctcgg ccgaggcgca ggatctgttc gggctcttcg acgacacggt gcggcgcctg
    11581 cttgccgcgc tgaagtaagg gggcggcgat gtccgggcat ctgttgcggg tgagcggtct
    11641 ggtggccggc taccgcgagc cggtgacggc tccggtcagc ttcacgctga agcggggcga
    11701 gatcctcggc atcgccggtc ccaacggggc cggtaaatcg accctgctca aggccctgtg
    11761 gggcggcgtg cgggtgtttg ccggcgccgt ggagaaggcg cccggcctga gcatttccca
    11821 ccaggcccag ggctttgacg atctgcgtgg ggtgcccctg tccgggcgcg agctgctgcg
    11881 cctgaccggc gcaccgccgg acggcctgcc gccgtgggtg caggagcgcc tcgaccggcg
    11941 cctcgaccgg ctgtccggcg gccagttcca gttcctgcgc ctgtgggcct gcctggccgc
    12001 gccggccgac ctgatcctgc tggacgaacc gtccaacaac ctggatacgg ccggcgtcgc
    12061 ctatcttgcg gactggcttg ggcgggctca cggcgatcag ggcatcgtga tcgtctccca
    12121 cgatgcggcg ctgatggcgc aggtgtgtac gcgcatcctg cacgtggacg cggtggaggt
    12181 ggcggcatga gcctcgaatt tctggtcgat cccctgttcc gcctgccctt cctcaccgga
    12241 ctcgccctgg cgggcctgtt gccggtgctg ggcatgtacc tgcgcctgcg caacgaatgg
    12301 ctggccgcgc tggccttcgc ccagatggcg tcaggcgggg cgctggtggc catggtggcc
    12361 gatgcgccca tgctgctggg cggcgccgtg gcggcgctgc tggcggccct ggtcaagggc
    12421 ttcgcggccc gcgccggggc cagcgcctat gccctgatga tgcttctcgg gtggggcggc
    12481 ggcgtgctcc tggtggccaa ccatcccctg gcggagcagg ccggccatgc cctcttcgac
    12541 gggcagttgt acttcaccgg gggggcgcac ctgttggccg ccctgattgc cgtgatcgtg
    12601 gcgatgcccg tgctcgcctg gctgtcgcgt ccgctgctgc tgggacgctt ctttcccgag
    12661 ttcttccgcg cccgtggcct ggacgagcgg ccgcggctgg tgctgttcga cctgatcgcg
    12721 ggattgtccg tggccctggc gacggtgagc ctgggagtga tggcagcgtt cgcgctggtg
    12781 ttcgtgccgc cgatggtggt gtttccgcgg gcgccgggct ggcgcgtcgg cctgctggtg
    12841 gcggccgtgg tggggatggg cggttatgtg acggccttcg ccctggcgct ggtgttcgat
    12901 cagcccttcg gaccggtctg cgcgattctg ctggtgctat tgtcaggcct cgtcggcctg
    12961 atcggacggc agtcataatt cacacctgtg agcgcaagta attcacggtt cgtcatggtg
    13021 ttgacggtgt tatcctaatc gttcatcccc tgttgaattc atctggagta cgttgtcgtg
    13081 gtgctaccgt ttttcggcaa gaagcccccg cctgccggaa gcagcacgcc ccgttcgagt
    13141 gcgcggagtc cgtccggatc atccgcggcg gcggcgcgtc cgggcctgcc ccagcccgat
    13201 cgaaacgaaa cgtcctccct cgacttcacc atcgccgggg gcgacatcaa gcgcgtgctg
    13261 gcccagtgtg ccgaccgtgt gcatgtggag gaaggcgtcg acgagatgtc gccgcctgca
    13321 gaagaagccg ccatcctgta tgccaacggt gcctggaacg aggcccgctc ggtgctggag
    13381 catgccctgc agaccaccgg gtccagcgat gcgcgcctgg gcgtgtgggt gatgctcctc
    13441 gatctgctgc ggctcaccgg ccagcgcgag cagttcgatg ccctgagtct ggccttcgcc
    13501 accgaattcg aacgctcgcc gccggcctgg caggatctgt ccagccagag cgcccgtccg
    13561 atggccagca agacacctct ggtcaatctt tccggcgccc tgtccggcca ggcggcagcg
    13621 cagttcgccc agctgatcca ggtcggcatg cgctcggggg ccctgcgcat cgacctcacg
    13681 cgcctgcgca gcatcgacca ggccggggcc gagctgctcc tgcgtgcggt gcgtggcctg
    13741 cgcaaggcgc gcatccgcct gtctctggtg ggcgcccccc atctggtgaa tctcgtctcc
    13801 gggcaggtgg tccgcggacg ggccgacaac aagacccagt ggctgttgct gcttgaggcg
    13861 ctgcaataca ccgaccagca ggagcgcttc gaggaactgg cggtggatta cgccatgacc
    13921 ttcgaggaat cgccaccgtc ctgggagccg ccgatggtca cggagagcca gaccgaggcg
    13981 gtcgaggcgg tcggcatgaa cgatatcttc gtgctggaag gcgaagtggt cggccccaat
    14041 gccgaagcgc tgcgcaagct ggccgccttt gccaacgacc gcaacaagat cgaggtggac
    14101 tgcgggcgcc tgcggcgcat ggacttcgtc agcgcgggca acctcttcaa catcgtctcc
    14161 cagttgcaca gcaagggccg cctggtggtg ctcaagagcg tcaatagcat ggtggcggca
    14221 ctgatgcagg tgatgagcct gcacgaagtc gcgcgcatcg agctgcgcgg ctgaattcgc
    14281 gcccaccggg tgggcgccct tggctgcccg cgttcgcggg ggcttgcatt ccttctcacc
    14341 gaccccatat ttccgcgatg gaacaatatc acggcaccac catcctctcg gtccggcgcg
    14401 gccgccacgt ggccctcggc ggcgacgggc aggtcaccct cggcaacatc gtcatcaagg
    14461 ccagtgcgcg caaggtgcgg cggctctacc aggaccgggt cctgatcggc ttcgccggcg
    14521 gcactgccga cgccttcacc ctcttcgagc gcttcgaggc caagctcgag aagcaccagg
    14581 gccacctgct gcgcagcgcc gtcgagctcg ccaaggactg gcgtaccgac cgtgccctgc
    14641 gccgcctgga ggccatgctg gcggtggccg atctggacaa ctccctgatc atcaccggca
    14701 acggcgacgt gctggagccg gagcagggca tcgttgccat cggcagcggc ggcgcctacg
    14761 cccaggcggc ggcccgcgcc ctcgtcgaga acaccgagct ggcgcccgaa gacgtggtca
    14821 agaaggccct cagcatcgcc ggcgacctgt gcatctacac caaccagagc cacaccatcg
    14881 aggttctgaa gccctgatcc cgaccccatg acccagatga ccccgccgga gatcgtctcc
    14941 gaactcgaca agcacatcgt cggccagtcc cgcgccaagc gcgccgtcgc cgtggccctg
    15001 cgcaaccgct ggcggcgcag ccaggtggcc gagcccctga agagcgagat cacccccaag
    15061 aacatcctga tgatcggccc caccggcgtc ggcaagaccg agatcgcccg ccgcctggcg
    15121 cgcctcgcca acgccccctt catcaaggtg gaggccacca agttcaccga ggtgggctac
    15181 gtggggcgcg atgtggacac catcatccgc gacctggtgg agatcgccat caagtcccac
    15241 cgggaagagg ccatgaaaaa ggtccgcgac cgcgcccagg acgccgccga ggaccgcatc
    15301 ctcgacgtgc tgctgccgcc ggcccggccc acgatgggct tcggcaatga agcgcccagc
    15361 gccgaggaga acgccacccg ccagaaattc cgcaagaagc tgcgggaggg cgacctggac
    15421 gacaaggaag tggacatcga ggtcagcgcc cccggcatga ccgccgagat cctcgccccg
    15481 ccgggcatgg aagagctcac cggccagctc cagagcatgt tccagaacct gggcggcggg
    15541 aagaagaagt cccgcaagct caagatccgc gaagccctca agctcctcac cgacgaggag
    15601 gccggccgcc tcgtgaacga cgaggacgtg aagctcgaag ccctgcgccg ggtggagcag
    15661 aacggcatcg tcttcctgga cgagatcgac aagatcgcca cccgctccga cagccacggc
    15721 gcggacgtct cccgccaggg cgtgcagcgc gacctgctgc ccctcgtgga aggcaccacc
    15781 atcagcacca agtacggcat ggtgaagacc gaccacatcc tcttcatcgc cagcggcgcc
    15841 ttccacctgt cccgcccgag cgacctgatc cccgagctgc agggccgctt ccccatccgc
    15901 gtggagctgg agtccctgtc cgtggaggac ttcgagcgca tcctcaccca gaccgacgcc
    15961 tgcctcacgc gccagtacga ggccctcctc gccaccgatg gcgtcagcct gagcttcgcg
    16021 ccggacggca tccgccgcct cgccgagatc gccttccagg tgaacgagaa gaccgagaac
    16081 atcggtgcgc gccggctcta taccgtcatg gaaaagctgc tggaagagct cgccttcgag
    16141 gctggccgcg ccggcaacca gaccgtcaac atcgacgccg ccttcgtgga cggacgcctc
    16201 gagacccttg cccagcgcga ggacctggcc cgctacgtgc tgtaagcgcg gcgtccggcg
    16261 cgcccttcgc ggcgtgccgg ggcgcggggc cgcgtgcgta tcctggctgc cccgtggcgg
    16321 ccgggtcggc cctacaatgg cggtccgccg gcatccgacc ggagattccc cttccggtca
    16381 tgctgctgcg catcgtttcc atcctcttcc ccctcttcgc cctcaccgcg ctcggctatc
    16441 tggtcggccg gcgccagcgg cccgacctct cccacgccaa caagctgaac atggacgtgt
    16501 tcgtgccggc gctgg
//
LOCUS       DAOQMM010000085        16482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1352_length_16482_cov_49.425945, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000085 DAOQMM010000000
VERSION     DAOQMM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16482
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_16482_cov_49.425945"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..626)
                     /locus_tag="PLH85_12945"
     CDS             complement(135..626)
                     /locus_tag="PLH85_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65519.1"
                     /translation="MRLKNLKSLVVAGCFVAGLVACESGPNVPAGEESKFLFALKTSE
                     TIGDQVGNVALNENGIMIHPGATAPTSVSFNLDGKLRAIGIKPYIAPLNEEGKAHADA
                     GIVGVELLVDGKSVDKFTVDRNFSQEKTLDVKGAKSLEIRVDNGNGTPIWDWFHVKVT
                     SVK"
     gene            complement(642..2591)
                     /locus_tag="PLH85_12950"
     CDS             complement(642..2591)
                     /locus_tag="PLH85_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65520.1"
                     /translation="MFFLSNQGDCGLEGFSPVYIRGCGLKVQGYSLDGKPWLQALFFV
                     LCAGVYLFLMNAHKAAELADDGAFFLRYAVNMLHGHFWVWNIGEAPVWGASAPLFPVF
                     LTLPLMFGFDPVTTVMNAGIVAGAAGLSVIAWLLAKRISLVAGLGFLAFSATSSQITY
                     WVGSGLETPLTIILLAFALFSIFGTDRPWIAGVAAGLLMIHKLDMVPVGLLVLAAAWL
                     RDRRVPATAAVIALLLSGAWYLFAWWYFGAPVPNSFITKALHQSHLVPIIDWRWYGQF
                     VLLSGINKYASVCLLGLAWAIRPSRPVLCLFGGLLLIHLTAYTIKHPVEPYAWYGMGA
                     MTSLFVLASVGLDGVARQLARLRSGHQGALLGDAVVLVFLVTAVLAVQRHERGLSEGF
                     KVWADNYERDRADAGRWVAAHTPESYVVMAGHGNPAYFSGRKVIDSSYLNRRYEAGNV
                     LERLRPEIYISPGNPSDPDFSKPLFSDYQVVKIFDRTFSSGVDSKYWFAVLARKDAVG
                     RMSGLNAGPTAPVSVEKYVSDVSLGDQFGVLRSEGSDTLFIHPGATTPTGFDFDASGF
                     AAGYGGRALQVTVSVSPKVPPEAVARGAAVARVTFSRKGEVAAQSIVKVGSPFHFTMP
                     ANSGWRVVVDNYNGPDTDWILISFN"
     gene            complement(2579..4315)
                     /locus_tag="PLH85_12955"
     CDS             complement(2579..4315)
                     /locus_tag="PLH85_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB65521.1"
                     /translation="MTVHQFPPEYISGTEVLTFSVAKDLMRRGHEVRVLTGAPAKAPL
                     RDAERFDEYEIEGIRVYRFTHEFVPMGDQNVIMEMEYTNRLVARYFQSLLDEFQPDLV
                     HIFHMSRLGTSIIDVATGMKIPTYFTPTDFWSACPTSLLMLDDGTPCKGPEGAGGNCV
                     RHVAMRARWSRIAKAAKYAPDFLADQVVTLARKGWLPRQMPFRNEISALSRRHEFVIS
                     RLNALNGIVSPTNLMTDALTRNGVRPELIRQHMYGINIDNYNDVIRAHVQGRTLTFGF
                     IGGLSSHKGCHVLIKAIRKLARADVRLKIYGNPKDSPAYFAELQSLADGAPFIEFCGT
                     FPNGKIAEVLSGIDALVVPSVWFENAPLVIHSALAARCPVVASNFPGMSEVVRHGENG
                     LLFEPGNPDSLADTLRRVLEEPTLLSELSSRCRPPKSIAEYVDEILAVYASPPAPEGG
                     RGIVEFRRFEPMDPRNEASFVSGWCIAKAGEPMSVEIRVNDRVVAQTRQFAPRPDVRA
                     GFGGAGLVIRGEQFGFALSFPAGIQRINASVRIAAPDGQTRDLSMSELAAGSMVKVGD
                     DFFIGLDQECFS"
     gene            complement(4324..7770)
                     /locus_tag="PLH85_12960"
     CDS             complement(4324..7770)
                     /locus_tag="PLH85_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB65522.1"
                     /translation="MTPALADAVIEEVLLACGGGTLAVVGQGAAHCVRAALRIGIDAR
                     GVEVSDAGAVEWARTAMPGRVVAGSVCALPYADGAVDTLVVLHGLETLDEDDVPTAIA
                     EIGRVASRAVYLRMATSPASGADGVFRSRGGWEQPLFSAGFRKHPSYYRVNAYEALEG
                     EAGEITIVLEKLPAPALKRYPLAALAEERDLHMDMLRETGARSDAHVARYQWAAAFVR
                     PGDTVLDAACGLGYGSYFLQTGTEAACTLGIDGSAYAIDYARENFSAIVPGLEFREGF
                     LPGALASIPDHSVDVVVSFETLEHVDDNLALLAEFHRVLTPAGRIVVSVPNDWSDETG
                     EDPNPFHVHVYTLERLRRELGAHFILEKLVAQSASQHKSGPDRTAWTAAGRSLRDVSP
                     DVADDQAPDAEWWLAVAMRSPLEGQDVPYRETQFPQFPSPDWNVTAFGRDYRNPWLVR
                     GMVDIAHRLQAPGALHVLAEEVAATAALDTADAGAALCVLAYQMLDAPETTCAAVEAL
                     DRRVMVYLDEDPPSPHGQRWCVSLQFVLGRLWMGVGDFARAAESLEGCIARGSDRFSP
                     LLDNRVVEACLILGQLNLHQGHRDSALRWWSTGIERARRAVGGDWSGAIGDLAHPAEF
                     GLPELASILEYASACAYALVHVDDVDVKPWWWLHPRRDRLSRHAAVLRELVCQREQLA
                     VRDRELAAYRAQSDAYREQLSRAVDAARDTAAYSERLAGELGSYQGQSDAYRQQLDAA
                     QTTTRELSGYAERLVRELAALKAESEEYARSVRKAQADVIDATASRAEVERALSESRN
                     VAATLERRQAEAEARLSAADRLVAGLQGEVAAYARESASYAEQIQAAEARLQESAAYA
                     STLLSQIEAYQRQSVEYCEQIRGAETQLQAASEHIASLQDQVVAYERQASQHAEQSRA
                     AEARLQEGAAYASTLLSQIEAYQRQSVEYCEQIRGAETQLQAASEHTASLLEHVAACA
                     GESQAYRTQLDEAVSRLSEFDAYVRRLEAELTAYQTQADVHEKRLAAVQQDADSAAAS
                     VRNLERDAVRMRDQLDREARRLGVSEATRTALERSLADAEGRVATFIEQEMVHRSRIG
                     ALESQVECLEQERNAHQVQYRSMLTLYSSRMASPRFLLRRLWHLILVRVGLIRRRESE
                     EQ"
     gene            complement(7791..9176)
                     /locus_tag="PLH85_12965"
     CDS             complement(7791..9176)
                     /locus_tag="PLH85_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB65523.1"
                     /translation="MSSEVVIRVAGLSKCYHIYDKPHHRLMQMLSRGRRTYFREFWAL
                     RNVSFEVRKGEAVGIIGRNGSGKSTLLQLICGTLNPTSGSVETRGRIAALLELGSGFN
                     PEFTGRENVFMNGAVLGLTREEMEARIADIEAFADIGEFIDQPSKTYSSGMLVRLAFA
                     VQVCIEPEILIVDEALAVGDALFQKRCFERMEHLRSNGTTLLFVSHDQESVRALTNRA
                     VFLHHGEVQHTGNSADVLLEYRRLLHEEETRALGEAAARLRNRAAEQRAQQAARPVAP
                     ATAASPAAVDAPAPSRSAELSFGDMDAEVVSVSVFNAGGEPCSHFDPRDVAQVVIQCV
                     ARRDLDRLNVGLRIRNKEGVKIYSWGTLNQDMAIRAGLADGEVFWNRSFKAGEVFSVS
                     LDFVVGLGPNLYEIQASITQEGKPYYAEQRMLHWMDEAAFFTVATDPRTYHFGGVVDM
                     RMSSRLLGGAS"
     gene            complement(9166..9918)
                     /locus_tag="PLH85_12970"
     CDS             complement(9166..9918)
                     /locus_tag="PLH85_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB65524.1"
                     /translation="MALARREVVGRYRGSFMGILWSFIHPVFMLAVYTFVFSVIFRAR
                     WGGGSEGREEFALVLFAGLLVFNVFSECVTQAPGLILSNVNYVKKVVFPLEILPGVTL
                     VSALFHCGISFVVWLVADVLLFGVPPVTVLLFPLVLLPLCLFVMGLSWLLASLGVYVR
                     DLAQFIGLATSVLMFLTPIFYPATALPEAYRAFLFLNPLTPVIEQARDVLCWGKGLDG
                     AEYALTLCASALFAWAGFAWFQKTRKGFADVL"
     gene            complement(10062..10970)
                     /locus_tag="PLH85_12975"
     CDS             complement(10062..10970)
                     /locus_tag="PLH85_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB65525.1"
                     /translation="MAILMGCYFGQDYLAAQLDSIQAQSYRHWKVWAADDGSTDATPE
                     LLERYQASWGRDRLAVRRGPQQGFVANFLALVCDDGIQADYFAYSDQDDVWAPEKLAN
                     AVAWLASVPAGIPALYCSRTRLFKGARTVGCSPRHRRPPSFANALVQNIAGGNTMVFN
                     RAARRLLAHAGPDVKVVAHDWWTYIVVTACGGQVHFDEQMTVDYRQHEDNQIGAGNGW
                     AFLVRRIEGFFNARFRCWVDGNLVALERLGACLSPESKRIFALFSAARKGGLSARLSG
                     LLRSGVHRQTAGGNCGLLIAALFNRI"
     gene            11263..13176
                     /gene="mnmC"
                     /locus_tag="PLH85_12980"
     CDS             11263..13176
                     /gene="mnmC"
                     /locus_tag="PLH85_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNB65526.1"
                     /translation="MSLIPARFTPGPANTPYSLQFDDVYHTASGGLGQARHVFIAGNE
                     LAARWKGRRTFTVLETGFGLGLNFLATWHAWAADADRCQHLHFASVEKHPLTRHDLAR
                     VLSDWPELAPQAAHLLNAWPNLVPGIHRFTFEQGRVVLSLALGDALQVLPELTMRADA
                     FYLDGFSPDRNPDMWSPAVFTQLARLAAPGATLSTWCVAGHVRRGVQEAGFIVERQPG
                     FGGKRHMLAGRFPGPGPTSTGIPDSHRALVIGAGVAGTSVAERLATRDWQITLIDSAD
                     APGQGASGNRAGVFRPQPSLDDNRLARLTRAGFLYGRQHLTQLAERGHPVRWAPTGVL
                     HLARDAKQENKMRAVVEAQQPPDGYLRYVTQEEAARLADWPVSAGGWWFPGGGWIQPP
                     SLCAASLAAGGTRIIPLFGRTLARLERRDGEWAALDADGHEIARAPIAILANGVGLRA
                     VPEAAMLPVRPARGQVSHLPAASGSAPGIVVCRLGYVSPEVDGVRCAGATFVADDLDP
                     QLRDSEHRDNLAKLDFILPGYAADYDPATLDGRVGFRPASPDRLPMVGAIPAVASVDR
                     TTPLADIPRQPGLFAVSGFGARGLVWASLMGELLASQLNGDPLPLERDLVDAIDPARY
                     LLKPPRPMRGEDG"
     gene            13328..14077
                     /locus_tag="PLH85_12985"
     CDS             13328..14077
                     /locus_tag="PLH85_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNB65527.1"
                     /translation="MDTSSPRPGLHPMLWVAAASVTALSLAGVARLTGLLPASSPEPA
                     PVVAVQPAPADTPVAPPAAPKVADAPVAEPPARVDHAPVPRHETPKPKAAAKTTPAPA
                     ATAQGAEPVVPAGRPVADVPPPPPVCRDCGVIESIETHQVKGEGSGLGAVGGALLGGI
                     LGNQVGEGSGKKLARIGGAVLGGFAGNEVERRERSTVRYDVTVRLDDGTRRVLTQSQA
                     PAWREGDRVRLHNGEILPYAPPASGAPRSSF"
     gene            14263..15621
                     /locus_tag="PLH85_12990"
     CDS             14263..15621
                     /locus_tag="PLH85_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65528.1"
                     /translation="MGFLPLIEAFLALGLTMIVLTTGVSSLAGTWQRLRRARARGLQS
                     LLRCTFLLAVKNIEKPASAGSEDPWQTFCAQRDAPDALIGFVAEMCLQPTALADCAAL
                     TGDARRAAYRRLLPDLAPAEAPSNGDSGRDASVPSRMAHLADRLLHAFRRWRARAFTL
                     DTLSDEEFRTRFTHSTLGAALCAADEAHFPARLDALNGQFIAYGHAASEAFAKDARLH
                     SLCCAAGLVILANIDAFNLLENYLAQPVLRAAIISRYEHAASGSSLPAGGATATTAVP
                     AVASQFDAVLRNAADAARKAKDLPLANAIEQKALEMRTSIDTLQTARNEALQTVADAT
                     AGLPVGWDRYPFCAAGASDTRCRGLTPPSPATDADARLRTRIEAFVGRVGEVADVDAG
                     NFLRWLAGLILTMVMLGLGTPFWVQAVNGLLRARDLVRGGNKAGDGPDGPPPAAAPPA
                     NR"
     gene            complement(15638..>16482)
                     /locus_tag="PLH85_12995"
     CDS             complement(15638..>16482)
                     /locus_tag="PLH85_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNB65529.1"
                     /translation="TYNFCVVVDDWDMGITHVIRGDDHVNNTPRQINILQALGAQVPL
                     YAHLSMILGDDGQKLSKRHGAVSVTQYDDDGFLPEAVINYLARLGWSHGDDEIFSRAQ
                     LVEWFDLDHITPSAAQFNTEKLKWLNAHYIKQSDTARLAADVARRLARRGVDPEHGPA
                     LEAVVALYKDRVSTLVELADAVEPYYIDLHPAPELLAQHLTDAARAALATLRGKLADV
                     AWEKALLNAAIKETCAAHGIKMPQVAIPLRVVLLGQPQTPSIDAVLETMGQALVLGRL
                     DRVL"
BASE COUNT         3011 a         5838 c         5000 g         2633 t
ORIGIN      
        1 aacacctgac cgcatatccc ttgtccagca acgcatccac cagatgcgac cctatgaacc
       61 cgcgcccgcc aattaccaga caacgcatct tccacctcaa atacggaccc gaaatcaggg
      121 aaccaaagac ctgattactt gacggacgtc acctttacat ggaaccagtc ccagatcggc
      181 gtgccgtttc cattgtcgac acgaatttca agcgacttcg cgccctttac atcaagggtc
      241 ttttcctggc tgaagttgcg atcgaccgtg aatttatcga cggattttcc atcgaccagc
      301 aattctacgc cgacaatacc tgcatcggca tgcgccttgc cttcttcatt aaggggcgca
      361 atgtaaggct tgatcccgat ggcacgcaat ttcccgtcca aattgaagct gacagacgtg
      421 ggcgcagtgg cgcccggatg gatcatgatc ccattttcat tcagcgcaac attcccgacc
      481 tgatcgccga tggtttcgga cgtcttcagt gcaaaaagaa acttgctttc ctcgcccgcc
      541 ggaacgttcg gaccggattc acaagccacc agccctgcga cgaaacagcc ggccacgaca
      601 agactcttaa gattcttcag acgcatttca agactcctca cctaattgaa cgaaatcaag
      661 atccagtccg tatccggacc gttgtaattg tcgacgacca cccgccatcc cgagtttgcg
      721 ggcatcgtga aatgaaaggg ggatcccact ttgacgatgg attgcgccgc cacctcaccc
      781 ttcctcgaga acgtcaccct ggcgacagcc gcgccccgcg caacggcctc cggaggcacc
      841 ttcggggaca ctgaaaccgt cacctggagg gctcgaccac cgtacccggc ggcaaagccg
      901 ctggcatcga aatcgaaacc tgtgggtgtc gtcgcgccgg gatggatgaa cagcgtgtca
      961 ctgccctccg acctcagcac accaaactgg tcacccaagg aaacatccga cacatacttc
     1021 tccaccgaca ccggggcagt cggaccggca ttcaggccgg acatccgccc gaccgcgtcc
     1081 ttgcgggcga gcaccgcaaa ccagtacttg gagtccaccc cgctcgagaa cgtcctgtcg
     1141 aatatcttga cgacctgata atcactgaag agcggcttcg agaaatccgg gtcagatgga
     1201 ttccccggcg agatgtagat ttcggggcga agacgttcca gcacgttccc ggcctcgtat
     1261 cgccggttga ggtaggagct gtcgatcacc ttacgcccgg agaaataggc ggggttgcca
     1321 tgccctgcca tcacgacata gctttccggc gtatgtgcag ccacccatcg cccggcatcc
     1381 gcacggtccc gctcatagtt gtcggcccag accttgaaac cctctgacag gcctcgctcg
     1441 tgacgctgta ccgccaggac cgccgtcaca aggaacacca gcaccaccgc gtcccccaag
     1501 agggccccct gatgacccga ccggagcctg gcaagctgtc gcgcaacccc gtccagtccg
     1561 accgatgcca gcacgaagag cgaagtcatc gcgcccatcc cgtaccaggc gtaaggctcg
     1621 acggggtgtt tgatcgtata ggcggtgaga tgaatgagca ggagcccccc gaacaggcac
     1681 aggacgggcc gtgagggcct gatcgcccac gcaagtccga gcaggcagac gctcgcgtat
     1741 ttgttgatac cgctcagcag cacgaactgg ccataccatc gccagtcgat gatcgggacc
     1801 aggtggctct ggtgcaaggc cttcgtaatg aaggaattcg gtaccggcgc cccgaaatac
     1861 caccacgcaa acagatacca cgccccgctg aggagaagcg cgatgaccgc cgccgtggct
     1921 ggaacacgcc gatcccgcag ccacgccgca gccaggacga gcagtccgac aggcaccatg
     1981 tcgagcttgt ggatcatgag gagcccggca gccacacctg cgatccatgg ccgatcggtt
     2041 ccaaaaatcg aaaaaagtgc aaaggccaga agaatgatcg tgagtggcgt ctccaggcct
     2101 gagcccaccc agtaggtgat ctggctgctg gtggcactga aagcgagaaa ccccagaccg
     2161 gccaccagac tgatgcgttt cgccagcagc caggcgatca ctgacaggcc cgccgctcca
     2221 gccacgattc cggcattcat caccgtcgtc acgggatcga agccaaacat caggggaagg
     2281 gtgaggaaga ccgggaaaag gggcgcgctc gcaccccaca ccggcgcctc accgatgttc
     2341 catacccaga aatgtccgtg cagcatattc actgcataac gcaggaaaaa tgcgccatcg
     2401 tccgcgagct ctgccgcctt gtgagcgttc atcaagaaca ggtaaacgcc ggcacagagc
     2461 acaaaaaaca aggcctgcag ccacggcttg ccgtccaacg agtatccctg cactttcaag
     2521 ccacaacccc ttatataaac cggcgaaaat ccttcgagcc cgcaatcacc ctgattgctc
     2581 aggaaaaaca ctcctggtcc aggccgatga agaaatcatc gcccaccttg accatgctgc
     2641 ccgcagcgag ctcggacatg gacagatccc gcgtctgtcc gtcgggtgcg gcaatccgca
     2701 cactggcatt gatccgctgg atacccgccg gaaaactgag cgcaaagccg aattgctcac
     2761 cccgaatcac gagtcctgcg cccccgaatc ctgcccgtac atcgggcctc ggcgcgaact
     2821 gacgggtttg ggccaccacc cggtcattga cgcgaatctc gacagacatc ggctcaccgg
     2881 ccttcgcaat gcaccagcca gagacaaaac tggcctcatt gcgtggatcc atgggctcga
     2941 agcggcggaa ttccacgatg ccccgcccgc cttcaggcgc aggaggcgaa gcatagacgg
     3001 ccaggatttc atccacatat tcggcaatcg acttcggcgg ccggcaccgg ctgctgagct
     3061 cgctcagaag cgtcggctcc tccaggacac ggcgcagcgt gtccgccaga ctgtccggat
     3121 tgcccggctc gaacagcaat ccgttttcac cgtgccggac gacttccgac atgccgggaa
     3181 aattggacgc caccaccggg caccgggccg ccagcgccga atgaatcacg aggggcgcgt
     3241 tctcgaacca gacggacgga accaccaagg cgtcgattcc actcaggacc tccgcgatct
     3301 tgccattcgg gaacgtcccg cagaactcaa tgaacggagc cccgtcggcg agtgactgca
     3361 gctcggcaaa gtaagccgga gaatccttcg gattgccgta gatcttcaac cggacatccg
     3421 cacgggccag cttccggatc gccttgatca ggacgtggca gcccttatgg gatgacagcc
     3481 cgccgatgaa tccgaatgtc aacgtcctgc cctgcacatg ggcgcggatc acgtcgttgt
     3541 aattgtcgat gttgatcccg tacatgtgct gccggatcaa ctcgggacgc accccgttac
     3601 gcgtcagcgc atcggtcatc agattggtgg gcgacacaat gccattgagc gcgttgagac
     3661 gggagatcac gaactcatgg cgcctcgaca gcgcggagat ctcgttgcgg aacggcattt
     3721 gtcgcggcaa ccaacccttg cgcgcgagcg tgacgacctg atccgcaagg aaatccgggg
     3781 catacttggc agctttggcg atccggctcc agcgcgcccg catggccaca tggcgcacgc
     3841 aattgccgcc ggccccctcg ggccccttgc acggggtgcc gtcgtccagc atgagcaggc
     3901 tggtcggaca ggcgctccag aaatcggtgg gcgtgaagta ggtgggaatc ttcatcccgg
     3961 tggccacgtc gatgatcgac gttcccaggc gggacatgtg gaagatatgc accagatcgg
     4021 gctgaaattc gtccagtaag gactggaaat agcgggccac caggcgattg gtgtactcca
     4081 tttccatgat cacgttctgg tcacccatcg gcacgaattc atgtgtgaac cgatagaccc
     4141 ggattccctc gatctcgtat tcatcgaagc gttcggcgtc ccgcaggggt gccttggcag
     4201 gggcccccgt cagcacgcgg acttcatggc cgcgacgcat cagatccttg gcgacgctga
     4261 aggtcaggac ttcggtcccg gatatgtatt ccggcggaaa ctgatggaca gtcaatacta
     4321 ttttcattgt tcttcactct cacgacggcg gatcaggcct acccgcacca ggatcaggtg
     4381 ccacaaacgg cgcaacagga aacggggact tgccatccgg ctggaataca gcgtcagcat
     4441 ggatcgatat tgaacctgat gggcatttcg ttcctgctcc aggcactcga cctgactttc
     4501 cagcgccccg attctactgc ggtgcaccat ttcttgctcg atgaatgtgg caaccctccc
     4561 ttcggcatcg gccagactgc gttcgagcgc cgtgcgcgtc gcctcggaga cacccagccg
     4621 tcgggcctca cgatccagtt gatcgcgcat cctgacggca tcacgctcaa gattccggac
     4681 ggatgccgcg gcactgtccg cgtcctgttg aactgcggca agccgtttct catggacatc
     4741 ggcctgggtc tgataggccg tcaattcagc ttccagccgc cggacatagg cgtcaaactc
     4801 gctcaggcgc gagacggcct catcaagctg ggtccggtaa gcctgtgact cgcccgcgca
     4861 cgcggccaca tgctcaagca ggctcgcggt gtgctcggac gccgcctgga gctgcgtttc
     4921 ggcgccccgg atctgctcgc aatactcgac cgactgacgc tggtaggcct cgatctggct
     4981 caggagcgtt gaagcgtacg cagcgccttc ctgaaggcgc gcctccgcgg cccggctctg
     5041 ttcggcgtgc tgcgatgcct gccgctcgta cgccacgacc tgatcctgca ggctcgcgat
     5101 gtgctcggac gccgcctgga gctgcgtttc ggcgccccgg atctgctcgc aatactcgac
     5161 cgactgacgc tggtaggcct cgatctggct caggagcgtt gaagcgtacg cagcgctttc
     5221 ctgaaggcgc gcctcggcag cctggatctg ctccgcgtac gacgcggact cccgcgcata
     5281 ggccgccacc tcgccctgca agcccgcaac cagacgatca gccgcgctca gccgggcctc
     5341 ggcctcggcc tgccgccgtt cgagcgtggc agcgacgttc cggctctcac tcaaggcgcg
     5401 ctccacctcc gcccgggagg ccgtcgcgtc gatgacatcg gcctgcgcct tgcggacgct
     5461 gcgggcgtat tcttccgact ctgccttcag cgcagccagc tcccggacca gccgttccgc
     5521 ataccctgaa agctcgcgcg tcgtcgtctg ggccgcgtcc agttgctgac ggtaggcatc
     5581 ggactgaccc tgatagcttc ccagttctcc cgccagacgt tcgctgtagg ccgccgtgtc
     5641 gcgcgcggcg tcgacggccc gcgacagttg ctcgcggtat gcatccgact gggcacggta
     5701 ggcagccagc tcccgatcac ggaccgccag ttgctcgcgc tgacagacca gctcgcgcaa
     5761 cacggcggcg tgccgggaca gacggtcgcg ccggggatgc agccaccacc agggcttgac
     5821 gtccacgtca tcgacatgga cgagcgcata ggcgcacgca ctggcatatt cgagaatcga
     5881 cgccagttcc ggcaagccga attcggccgg atgggccaga tcgccgatcg cgcctgacca
     5941 atcccccccc accgccctgc gggcccgctc gatcccggtg gaccaccatc gcagtgcgga
     6001 gtcgcgatgc ccttggtgca ggttcaactg cccaagaatg aggcaggctt ccaccacccg
     6061 gttgtccagc agcggactga agcgatccga cccccgcgcg atgcagcctt ccagggattc
     6121 ggccgcacgg gcgaaatcac ccacgcccat ccacagccgc cccagcacga actgcaagga
     6181 cacgcaccaa cgctggccgt gcggactggg gggatcctcg tccaggtaga ccatcacgcg
     6241 acggtccaga gcctccacgg cggcgcacgt ggtttccggt gcatccagca tctggtaggc
     6301 cagcacgcat aaggcagcgc ccgcgtcggc ggtatcgagg gcggccgtcg cggccacttc
     6361 ttcggcgagc acgtgcaatg ccccgggcgc ctgaagccga tgggcgatat ccaccatgcc
     6421 ccggaccagc cagggattcc ggtaatcccg cccgaatgcc gtaacgttcc agtccggcga
     6481 cggaaactgc gggaactggg tctcccggta cggcacgtcc tgcccctcca ggggcgaacg
     6541 catcgccacg gcaagccacc attccgcgtc gggggcctga tcgtcggcca cgtccggcga
     6601 tacgtcccgc agcgagcgcc cggccgccgt ccaggccgtg cggtcggggc cgctcttgtg
     6661 ctggctggcc gactgggcca cgagcttttc gaggatgaaa tgtgccccca attcccggcg
     6721 caggcgctcc agtgtgtaga cgtgcacgtg gaagggattc gggtcctcgc cggtttcatc
     6781 gctccagtcg ttgggcacgc tgacgacgat gcgtccggca ggggtcagga cgcggtgaaa
     6841 ctcggcgagg agtgcgagat tgtcatccac gtgctccagc gtctcgaagg acaccaccac
     6901 gtcgacagaa tgatcgggga tcgatgcgag cgcgcccggc aggaagcctt cgcgaaactc
     6961 caggcccggc acgatcgctg aaaagttctc acgggcgtaa tcgatcgcgt acgcgctgcc
     7021 gtcgataccg agggtgcacg ccgcctcggt cccggtctgc aggaaatagc tgccatagcc
     7081 caggccgcag gctgcgtcga gcacggtgtc gccggggcgc acgaaggccg cagcccactg
     7141 atagcgggcc acgtgggcgt ccgagcgggc gcccgtctcg cgcagcatgt ccatatgcag
     7201 atcgcgctct tccgccagcg cggccagcgg atagcgtttc agcgcgggcg cgggcagctt
     7261 ttccaggacg atggtgattt cgcccgcctc cccctccagc gcctcgtagg cattgacgcg
     7321 gtagtaagac gggtgcttgc ggaaaccggc actgaacagt ggctgctccc acccgccccg
     7381 cgaccggaac acgccatcgg caccggacgc cggggatgtg gccatcctca gatagaccgc
     7441 gcgggacgcc acccgaccga tctccgcgat ggccgtcggg acgtcgtctt cgtccagcgt
     7501 ctcgaggccg tgcaggacaa ccagcgtatc cacagcgccg tcggcgtagg ggagcgcaca
     7561 taccgagccg gccacgacgc gacccggcat cgccgttcgc gcccattcaa ctgcgcccgc
     7621 atcggagacc tccacccccc gggcgtcgat cccgatgcgc agggcggcgc ggacgcagtg
     7681 cgccgcgccc tgaccgacaa ccgccagcgt tccgcccccg caggcgagca ggacctcctc
     7741 gatcaccgca tcggcaagcg cgggggtcat tcccccggcg ggcgttccgc tcatgaagcc
     7801 ccccccagga gccggctgga catgcgcatg tccaccacgc cgccgaaatg gtaggtgcgc
     7861 ggatccgtcg ccaccgtgaa gaaggccgcc tcgtccatcc agtggagcat gcgctgctcg
     7921 gcgtaatacg gtttgccttc ctgagtgatg ctggcctgga tctcatacag gttcggtccc
     7981 agcccgacga cgaaatccag gctcaccgaa aacacctctc ccgccttgaa cgaacggttc
     8041 cagaacacct cgccgtccgc cagcccggcg cggatcgcca tgtcctggtt gagcgtgccc
     8101 caggagtaga tcttgacgcc ttccttgttg cggatgcgca gacccacatt gagccggtcg
     8161 agatcgcgcc gcgccacgca ctggatgacg acctgcgcca catcccgcgg atcgaaatgg
     8221 gaacagggct cgccaccggc attgaacacc gacaccgaca ccacctcagc gtccatgtcg
     8281 ccgaaggaca gctccgccga gcgggagggc gccggggcgt ccaccgctgc ggggctcgcc
     8341 gccgtggccg gtgccaccgg gcgggcggcc tgctgagcgc gctgctccgc cgcccggttg
     8401 cgcaggcgcg cggcggcctc gcccagggcg cgcgtctcct cctcgtgcag gagccggcga
     8461 tattccagca gcacgtccgc cgagttcccg gtgtgctgga cctccccgtg atgaaggaag
     8521 acggcgcgat tggtcagcgc acggacggac tcctgatcat gggacacgaa cagcagcgtc
     8581 gtcccgttgc tgcgcaaatg ctccatccgc tcgaagcagc gcttctggaa cagggcatcg
     8641 cccaccgcca atgcttcgtc gacgatcagg atctccggct cgatgcacac ctgcaccgca
     8701 aaggccagcc gcaccagcat cccgctcgaa taggtcttgc tgggctggtc gatgaactcg
     8761 ccaatatccg caaaagcctc gatgtcggcg atccgggctt ccatctcctc ccgcgtgagc
     8821 ccgagcacgg cgccgttcat gaagacgttt tcccgcccgg tgaactccgg gttgaatccc
     8881 gacccgagct ccagcagggc ggcgatccgg ccacgggttt caacgctccc cgaggtcggg
     8941 ttcagggtgc cgcagatgag ctgcaggagg gtggacttcc ctgagccgtt ccgcccgatg
     9001 atgcccaccg cctcgccctt gcgcacctcg aaggagacgt tgcgcaaagc ccagaattcc
     9061 cggaaatacg tgcgcctgcc ccgggacagc atctgcatca ggcggtgatg gggcttgtcg
     9121 tagatgtgat agcacttgct cagccccgcc acgcggatga cgacctcaga ggacatcggc
     9181 aaaccccttc cgggtcttct ggaaccacgc aaatccggcc cacgcaaaca gcgcgctcgc
     9241 acacagggtc agggcatatt ccgcgccatc gagccccttg ccccagcaca ggacgtcgcg
     9301 cgcctgttca atgaccgggg tgagcggatt caggaaaagg aatgcccggt acgcctccgg
     9361 cagggccgtg gccgggtaga agatcggcgt caggaacatc agcaccgagg tcgccagccc
     9421 gatgaactgg gcgagatccc gcacgtacac gcccagggac gccaacagcc aggacaggcc
     9481 catcacgaac aggcacaagg gaagcaggac gagcggaaac agcagcaccg tcaccggcgg
     9541 cacgccgaac agcaggacgt cggccaccag ccataccaca aagctgatgc cgcaatggaa
     9601 gagcgccgat acaagagtca cgccgggcag gatctcgagg gggaagacca ccttcttgac
     9661 gtagttgacg ttgctcagga tcagccccgg cgcctgtgtg acgcattccg agaacacgtt
     9721 gaacaccagc agcccggcaa acaggaccag cgcaaactcc tcccgcccct cactgccgcc
     9781 cccccagcgc gcccggaaaa tcacgctgaa gacaaaggtg tagacggcaa gcatgaagac
     9841 cgggtggatg aaggaccaca ggatgcccat gaaggagccc cgatagcgcc cgacgacctc
     9901 gcgcctggcc agggccatca ggagattccg gtgactccag aatcccatga gcatccccgc
     9961 gggactggac gtgaatttct gcattcaaac ctctcaggct ctgccagcgc cggtcgtgcg
    10021 atcgctcagc ttccgcccgc gcctcccgtg agccgtcatt gctaaatcct gttgaagagt
    10081 gcggcgatca gcagtccgca attcccgcca gccgtctgcc gatgtacacc ggatctcagc
    10141 aaccccgaaa gcctcgcaga caacccgcct ttccgagcgg ccgaaaacaa ggcaaaaatc
    10201 cgcttacttt ccggtgaaag gcacgcaccc aggcgctcga gggccacgag attgccatcc
    10261 acccaacacc ggaagcgcgc gttgaagaat ccttcgatac ggcgcacgag gaatgcccag
    10321 ccattgccgg caccaatctg attgtcctcg tgctggcgat agtcaacggt catttgttcg
    10381 tcgaaatgca cctgtccgcc gcaggcagtc acgacaatat aggtccacca atcgtgggcg
    10441 acgaccttca cgtcgggacc ggcgtgtgcc agcaggcgac gtgccgcccg gttgaagacc
    10501 atcgtattgc cacccgcaat attctgaacc agcgcatttg caaaggacgg cggccgccga
    10561 tgtcggggcg aacagccaac agtccgcgcc cctttgaaca gccgcgtccg ggaacaatag
    10621 agcgccggta ttccagccgg cacagacgcc agccaggcca cggcattggc gagcttttcc
    10681 ggcgcccaca cgtcatcctg gtccgaatat gcgaaatagt cggcctgaat cccgtcatcg
    10741 cagaccaggg ccaggaaatt cgcgacgaat ccctgttgcg gcccccgtcg caccgccagc
    10801 cggtcgcgcc cccaggacgc ctgataacgc tcgagcaatt ccggcgtggc atcggtggaa
    10861 ccgtcgtccg ccgcccagac cttccagtga cgatacgact gggcctggat cgaatcgagt
    10921 tgcgccgcca gatagtcctg cccaaaatag cagcccatga gaatggccac ttccggcgat
    10981 ccgggggact ccggcgccag gctgcccgta ttcgggcgcg acgctgaatc tggcaagcaa
    11041 ggcacgtggg gaaaatgacg ggaaggcccg aaaaggcgca gtcagacaga aaatcagaca
    11101 gttatccaga atagcgggaa gagtatagca tcttggccag tcatcctcga ccccggcctt
    11161 cctcgacccg gccccgcgcc catgtgcagt gcggggcgcc tgagttcacg cgcccgacga
    11221 ctcaggcaca gcatctcgac gtcttcactg tagctagatt ccatgtccct cattcccgcc
    11281 cgtttcaccc ccggcccggc aaacacaccc tactccctgc aattcgatga cgtttaccac
    11341 actgcctcgg gcggactggg ccaggcccgc cacgtattca tcgcggggaa cgaactcgca
    11401 gcgcgctgga agggccgccg gaccttcacg gtgctcgaaa cgggattcgg actcggtctg
    11461 aattttctcg ccacctggca cgcctgggca gccgatgcag accgctgtca gcacctgcat
    11521 ttcgcctccg tggagaagca ccccctcacg cggcacgatc tggcgcgcgt cctgagtgac
    11581 tggcccgaac tcgcaccgca ggcggcacac ctgctgaacg catggccgaa tctggtgcca
    11641 ggcattcatc gattcacctt tgagcaaggt cgggtggtcc tcagtctggc cctgggcgat
    11701 gcccttcagg tcctgcccga gctgaccatg cgggccgacg ccttctacct cgacggtttc
    11761 tcccccgacc gcaacccgga catgtggtcc cccgccgtct ttacccaact ggcgcgcctc
    11821 gccgcccccg gcgccaccct gtccacctgg tgcgtcgccg gccacgtgcg gcgcggcgtg
    11881 caggaggcgg gcttcatcgt cgaacgacag ccgggcttcg gcggcaagcg ccacatgctt
    11941 gcgggccgtt ttcccggtcc ggggccgacg agcaccggca tcccggattc ccaccgcgcc
    12001 ctggtcattg gcgccggcgt cgccggtaca tcggtcgccg aacgcctcgc tacccgtgac
    12061 tggcagatca ccctcatcga cagcgccgac gcgcccggcc agggcgcctc gggcaaccgg
    12121 gcgggggtct tccggccgca gcccagcctg gacgacaacc gcctcgcccg cctcacccgc
    12181 gccggctttc tgtacggccg gcagcatctc acccagctgg ccgaacgcgg ccatccagtg
    12241 cgctgggctc cgaccggggt gctccacctc gcccgcgacg ccaagcagga aaacaagatg
    12301 cgtgccgtcg tggaggccca gcagcccccc gacggctacc tgcgttacgt cacgcaggaa
    12361 gaggcggccc gcctcgccga ctggcccgtg agcgccggcg gctggtggtt tcccggcggt
    12421 ggctggatcc agccgcccag cctgtgcgcc gccagtctgg cggcgggcgg cacacgcatc
    12481 atccccttgt tcggccgcac cctcgcccgc ctggaacgtc gcgacggcga atgggccgcc
    12541 ctcgacgcgg acggccacga gatcgcccgc gcgccgatcg ccattctggc caacggcgtc
    12601 ggcctgcgcg cggtgccgga agccgccatg ctgcccgtgc gccctgcccg cggacaggtc
    12661 tcccacctgc cagccgcgtc cggcagcgcg cccggcatcg tggtgtgcag gctgggttac
    12721 gtcagcccgg aggtggacgg cgtacgctgc gccggcgcca ccttcgtcgc cgacgacctg
    12781 gacccgcaac tgcgcgacag cgaacaccgg gacaacctcg ccaaactgga tttcatcctg
    12841 cccggctacg ctgcggacta cgatcccgcc accctcgacg gtcgcgtcgg cttccgcccc
    12901 gcctcgccgg accgcctgcc catggtgggc gccatcccgg ccgtggcgtc cgtcgacagg
    12961 accacgcccc tcgccgacat tccccgccag ccgggcctct tcgcggtgag cggctttggc
    13021 gcccgcggcc tggtgtgggc gagcctgatg ggcgaactcc tcgccagcca gttgaacggc
    13081 gatcccctgc ctctggagcg ggatctcgtg gacgccatcg atcccgcccg ctacctgctc
    13141 aagccgccgc gccccatgcg gggcgaggac ggctgaacgt ccgcgccggc cctccgtctg
    13201 cgcacccatg tgcccgcgcg gcacgaatat ccgtacgctg cagcattgta agcgccgatt
    13261 tcaaacgtca cctgagcgac ctgccgcgcg ttatcctgat caacacattc cgcaggagca
    13321 catcaccatg gacacctctt ccccccgccc cggactgcat ccgatgctgt gggtcgccgc
    13381 cgcctcggtg accgccctca gtctggccgg cgttgccaga ctcacaggac tgctgcccgc
    13441 ctcgtcgccc gaacccgcgc cggtcgtggc cgtccagccc gccccggccg atacacccgt
    13501 cgcgcccccg gcagcgccca aggtcgccga tgcacccgtg gccgaaccgc cggcccgtgt
    13561 ggaccacgca ccggtacccc gtcacgaaac gccgaagccc aaggcggccg ccaagaccac
    13621 gccggcgccg gcagccacgg cccagggtgc ggagccggtc gtccccgccg gccgccccgt
    13681 ggccgatgtc ccgccgccgc cgccggtctg tcgcgactgc ggcgtgatcg agagcatcga
    13741 gacccaccag gtcaagggtg aagggtccgg cctgggtgcc gtcggcggcg ccctcctcgg
    13801 cggcatcctg ggcaaccagg tcggcgaagg ctccggcaag aagctggccc gcatcggtgg
    13861 cgccgtgctg gggggattcg ccggcaacga ggtcgagcgc cgcgaacgca gcaccgtgcg
    13921 ctacgacgtc accgtccgcc tggacgacgg cacccgccgc gtcctgaccc agagccaggc
    13981 ccccgcctgg cgtgagggcg accgggtgcg cctccacaac ggcgagatcc tcccctacgc
    14041 cccgcccgcc agcggcgcgc cccgcagcag cttctgacag accggcccgt ccgggcctcc
    14101 cctgtccgcg gccccgtcga tctcccgatc ggtggggccg tcgtgtttaa gggcgggcgc
    14161 tcgccacggc gcttgcgccg ccagatgctt cagctactgt ggacagcatg cacatgacgt
    14221 tgcgctgcga cgaagctgtg acctgttgac ggagtacatg cgatgggctt cctgcccctg
    14281 atcgaagcat ttctggccct cggcctgacc atgatcgtcc tcaccaccgg cgtgtcctcc
    14341 ctggccggca cctggcagcg cctgcgccgc gcccgcgcgc gtggcctgca gtcgctgctg
    14401 cgctgcacct tcctgctcgc ggtgaagaac atcgagaaac ccgcatccgc cggatccgag
    14461 gacccgtggc agacattctg cgcgcagcgt gatgcaccgg acgccctgat cggcttcgtc
    14521 gccgagatgt gcctgcaacc caccgctctg gccgattgcg cggccctgac aggcgacgcg
    14581 aggcgcgcgg cctacagacg actgctgccc gacctcgccc ccgccgaagc cccgtcgaac
    14641 ggggactccg ggcgcgacgc gtcggtgccg agccggatgg cacaccttgc cgaccggctg
    14701 cttcacgcct ttcgccgatg gcgtgcgcgg gcattcaccc tcgataccct cagcgacgag
    14761 gagttccgca cgcgctttac ccactccacc ctcggcgccg ccctctgcgc cgcagacgag
    14821 gcgcattttc ccgcccggct cgacgcgctg aatggccagt tcatcgccta cgggcatgcc
    14881 gcaagcgaag ccttcgccaa ggatgcccgg ctgcacagcc tgtgctgcgc cgccggcctg
    14941 gtgatactgg ccaacatcga cgcgttcaac ctgctggaaa actacctcgc ccagcccgtc
    15001 ctgcgggccg ccatcatcag ccgctacgag catgcggcca gcggatcgtc cctccccgcc
    15061 ggcggggcga ccgccaccac cgcggtgcct gccgtcgcgt cccagttcga cgcggtcctg
    15121 cgcaacgccg ccgacgctgc ccggaaggcc aaggacctcc ccctcgccaa tgccatcgag
    15181 caaaaggcgc ttgaaatgcg gacctcgatc gacacgctgc agaccgcccg caacgaagcc
    15241 ctgcagaccg ttgccgacgc gacggccgga ttgcccgtgg gctgggaccg ctatcccttc
    15301 tgcgcggcag gcgcctcgga cacccgctgc agagggctga cgccgccgtc gccagcgacg
    15361 gacgccgacg cacgcttgcg cacgcgcatc gaggcctttg tgggccgcgt cggtgaggtc
    15421 gcggacgtgg atgccggcaa cttcctgcgc tggctcgccg gactgatcct gaccatggtg
    15481 atgctcggtc tcggcacacc cttctgggtc caggccgtca acggcctgct gcgggcacgg
    15541 gatctggtcc gcggcggcaa caaggccggc gatgggcccg atggcccccc gcccgccgcc
    15601 gcaccgcccg ccaaccgctg atcggcgcgt cgcgccctta cagcacccgg tccaggcgcc
    15661 cgagcacgag cgcctgcccc atggtctcca gcaccgcgtc gatggacggg gtctggggct
    15721 ggccgaggag gacgacgcgc agcgggatcg ccacctgggg catcttgatg ccgtgggcgg
    15781 cgcaggtttc cttgatggcg gcgttgagca gggccttctc ccacgccacg tcagcgagct
    15841 tgccgcgcag ggtcgcgagg gccgcgcggg cggcgtcggt gaggtgctgg gcgaggagct
    15901 ccggcgccgg atgcaggtcg atgtaatagg gctcgaccgc gtccgccagt tccaccagcg
    15961 tggacacccg gtccttgtac agcgccacca ccgcctccag cgcggggccg tgctccggat
    16021 ccacgccacg ccgggccagg cggcgggcca cgtcggcggc gagtcgggcg gtgtcgcttt
    16081 gcttgatgta gtgggcgttg agccacttga gcttttcggt gttgaactgg gcggcggacg
    16141 gggtgatgtg gtccaggtcg aaccactcca ccagttgcgc gcggctgaag atctcgtcgt
    16201 cgccatggct ccagcccagg cgcgccaggt agttgatgac cgcctccggc aggaagccgt
    16261 cgtcgtcgta ctgggtgacg ctcaccgcgc cgtggcgctt ggacagcttc tggccgtcgt
    16321 cgccgaggat catggagagg tgcgcataaa gcggcacctg ggcgcccagg gcctgcagga
    16381 tgttgatctg gcgcggcgtg ttgttcacgt ggtcgtcgcc gcggatgacg tgggtgatgc
    16441 ccatgtccca gtcgtccacc accacgcaga agttgtaggt gg
//
LOCUS       DAOQMM010000086        16434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1366_length_16434_cov_33.827767, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000086 DAOQMM010000000
VERSION     DAOQMM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16434
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1366_length_16434_cov_33.827767"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /gene="mutL"
                     /locus_tag="PLH85_13000"
     CDS             complement(<1..1583)
                     /gene="mutL"
                     /locus_tag="PLH85_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNB65530.1"
                     /translation="MPIQVLPDHLINQIAAGEVVERPASVLKEVLENALDAGATALDV
                     QLDQGGVRRIRVADDGGGIPKDELPLALERHATSKIASLDDLERVGTMGFRGEALAAI
                     AAVSRLSVTSRADGAPHAWRIDAHPRELAPAALNHGTVVDVADLYFNTPARRKFLKSE
                     GTEFAHCDDVFRRVALARPDVALQLTHNGRVGLRLPAGPREARVRALLGDDFLAAARA
                     VEAASGLLRLHGYASLPAYARSSRDAQFFYVNGRFVRDKLIAHAIREAYADILHGSRH
                     PAFVLFLELDPFGVDVNVHPAKTEVRFRDARAVHQFVFHALTRALSASGAAQTGEPDR
                     DGAGITPRPVPSHAGGAPYAPAPQQGRLAMEPVSRAYFDFAAAARPAGPAPVGASPGV
                     SAGAVGNGPETPHAPLPASDLAPPLGYALAQLHGVYILAQNEAGLILVDMHAAHERIL
                     YERLKTLLDGTPGVQRLLIPAVFGVGAQDMASAEEHAELLARMGFEIGPAGPQELAVR
                     SIPALLAAAPVGELVRALLA"
     gene            1688..2539
                     /locus_tag="PLH85_13005"
     CDS             1688..2539
                     /locus_tag="PLH85_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65531.1"
                     /translation="MQLTYIRPQGRLAEVVAGYLFASTLDESWPTDMPWLSSIPAGNE
                     WALTVFTEGRAVLLGDGLAPPQVLPSWVLSGPRLRPVTLMNLCPLRAVSVLFHGDALA
                     RLVNLAPVHLPQGCVDPAELLGADWALFRSALGARAGDRVAQQNWLEAFLEPRWEARR
                     LGLSRAPTATVRAAGRIAAAASARAMGWHERCIERSGPRAIAVTPAVGRHVALIHGAL
                     MEAWGLGPAAGARRLGDMARALGVAHPAGLVRALAARTWVGSERATSAESASPPEALR
                     SPVRPAG"
     gene            complement(2511..3722)
                     /locus_tag="PLH85_13010"
     CDS             complement(2511..3722)
                     /locus_tag="PLH85_13010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNB65532.1"
                     /translation="MRLSPLASLASLLTGALAVVATSTAHADTPKRYIVQFQDTAVQA
                     PPRGPVHDRRADEDPRGWGYVDSRVIERVQSTESRHGIQALHAYSSVIKGFAAALSDA
                     QVARLKADPTVLSIEEDLPMHTTAQTIPWGISTTRATLSSTLAGDGSGTVSTVNVYVI
                     DTGIAPHGELNKINHVNFTGDGKNTDCNGHGTHVSGTIGARDNTADVVGMAPAVALTG
                     VKVLDCTGSGSTSTVIKGIDWVTANAVKPAVANMSLGGSASTTLDAAVTKAVNAGIVF
                     AIAAGNSAANACNYSPARVGGSLAGAITTAATDSANKEASFSNYGSCVDTWAPGVSIL
                     STYLNAGMATMSGTSMATPHVTGAAALYLSRNPNATPAAVEAAIKQAAVAPGTVSKDG
                     RAITRLDVLGF"
     gene            3830..4684
                     /locus_tag="PLH85_13015"
     CDS             3830..4684
                     /locus_tag="PLH85_13015"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HNB65533.1"
                     /translation="MPPYPRSVAPAGRAVLDHALVILRPLVAWLLRSGVGYGEFAAAL
                     KPVFLDMALAEVARGGTKLTDSALSLLSGLHRKDVRALVPVLQGAADPSAALGKPSPA
                     SQVATRWLTNGWPERLPVAGPEPSFEALARSVSQDFHPRAVLLELIRLGVVEEADGAV
                     HLRQAAFVPDARHEETRRLMAGSVADHLAAGVHNLTEDGSRRFLEQSVFADGLSAESA
                     RALEQLANALWQDVLTAMVQAAVPLCEKDEAAGGDQRIRLGMFCYSAPMAGAAAPDAA
                     ADEKDPPV"
     gene            4681..6171
                     /locus_tag="PLH85_13020"
     CDS             4681..6171
                     /locus_tag="PLH85_13020"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNB65534.1"
                     /translation="MKTRFLAPVSGRLARWAARPRLSAAMFLIALLSACGGGGAFDVA
                     GVGSGGSGVASGTVTGFGSVIVDGVEYDDSSASRQSEDATGSLANAPVKLGQQVQLNY
                     SGSRVAQRIEVIAQLLGPVTAAPGSDGRLAVMGQPVRVVTSVSDASQSTPTVLDGYAA
                     VTAIAVGDEVEVHGAWVYDSAVASNVLVATRVEKRSVAADPVQLGGVVTAVGSGTLRL
                     NSATGTQVSGSDLAEIAVGQVVRVWAARSALAASPVVARRVAVQGVTASELGTAGGTL
                     SGLASQYDPASRSVSIQGVRVKLPDSMTVDSAALARGEFVTVQVTASGSTLVASTVAQ
                     RSGSAGADLGRTVEVKGVTRGIDWSATAVSFTLRDTPIVAAAAAIDASCRSVSPTASV
                     YVEVRGALAQSAATVTANSVKCSQSVPGSATVDYTGTIAAIDASARTLTLRVDVSGAS
                     VSGTWDAQTYFEQRPASLPVGLRVEVEGVMDQGSSTLRLSKVRLED"
     gene            6235..7818
                     /locus_tag="PLH85_13025"
     CDS             6235..7818
                     /locus_tag="PLH85_13025"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNB65535.1"
                     /translation="MNLFFNLPRRERVLGVVPVALALALAGCGGGGGTSSASASSSST
                     ATESSSGTVTGFGSVFVDGVELEDAKAVTRVENADGSYSLTSTQLGQRVRVAHDATGT
                     ASTVTVDAAVIGTVASGSVDATALTFTVAGQSVKANSDSTSGPLTVYGGGYTAFSDIA
                     AGDLVEIHGSAVYDSTAKVYVVQATRIEKKSAISAVRVMGKIASLDTTAKTFAINSLT
                     VAYGSAILAPTTATLADGVTVVAWGAQNSLVSGSSGLSLTASRVRVLNAGLADDSASG
                     ATQLGGVVSNYSVSAGTFELEGVKVIVGSATLSPTGATIANGAYVQVSGTVGSDASIT
                     ATSIKVRQQSTTDDTASVRLIGAIESLSDQTSFVVRGVPVDASAITLSTACPGVTLAV
                     GTVVKVTATVQSGTDVVKATSLGCTSATTYTMLSASGTAGTVNTTVRTFVLTTTGTAT
                     TTRTVQWSDQTVFAGVTAATLDGVTVAVEGYLDSSGVLVARKIRNPAVAAGREDADAY
                     AGVDDGSGRSWSRYRSTHR"
     gene            complement(7928..9253)
                     /locus_tag="PLH85_13030"
     CDS             complement(7928..9253)
                     /locus_tag="PLH85_13030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HNB65536.1"
                     /translation="MDRRTLRAALTDFLPEVHALEHAEPPHGPRVLLYALLALVVTAL
                     VWASVSEIDILAIGRGRLVTPLPNLVVQPLEAGILKSIDVRVGQQVRQGTVLATLDPT
                     FAQADAGQLGARRDSLAQRAQRLEAELHGNPATPSTAAGQEGVQARLMAERQAAHAAR
                     LKQYDESIARLRAALETNRRDEATLAERVHSLAELEAMQAELEARQFGSRAKVLELRE
                     RKLEVERDRNIAAHRDQELRREIAAAEAERTGYLRTWRQDSLEKLSQTVQERDEVTAQ
                     LDKTRLRTDLIRLTAPQDGVVLEIGKRSVGSVLKEAEPLFTLVPMNAPLEAEVEIGPG
                     DVGLIRPGDTVRIKFDTYPFQKHGTARGRVLHVSPDAFSRQGPGGATTSYYLARVALD
                     DTRLDGIAQPTLLLPGMSLTGEIVVGRRSVIAFFLYPLIRTLDESLRER"
     gene            complement(9256..11412)
                     /locus_tag="PLH85_13035"
     CDS             complement(9256..11412)
                     /locus_tag="PLH85_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNB65537.1"
                     /translation="MDSLTPFETALRSLTAVARHHRVNTSPEQIRRAHPAAAGTPADA
                     LPTLVAAARAIGLKARSLKTDFPALPPLQDTFPALARLNNGNWVVLVGLRGQGEEAAV
                     AVLDPLATPADAVFLIDRARFEAAWSGDLVLVTRVYSLHDENRPFGLGWFVPELLRQK
                     AFLRDVAVAALVLHVLALAMPVFTQLVIDKVIVHQGYSTLYVLTAGVILALLFEAAFS
                     YLRQTFMLQATNRLDVRLARKTFAHLLRLPITFFETGTAGVITRHMQQAEKIRQFLTG
                     RLFSTLLDASVLVLLLPVMFFYSGLLATLVLAFAAAIGAVVVALIPAFRARLQALYSA
                     EAERQSLLVETIQGMRTVKALAVEPLRRDDWDARSARTVALHFRVGRISLAAHTAMSL
                     LEKLMGVSVIAVGANLVFDQTLSVGSLIAFQMLSGRVVSPLVQIVALVHEYQETALSV
                     RMLGEIMNRPVEGQRASGGLEPTLEGHIAFENVSFRYAPGAAAALEQISFSLPAGKVV
                     GVVGRSGSGKTTLTRLMQGLYPVQDGIVRFDGIDLREIDLAHLRRQIGVVLQDNFLFR
                     GTVRDNLSITRPTAGFDEIVAAARLAGADEFIERLPQGYDTLLEENAANLSGGQRQRL
                     AIARALLNRPRILILDEAASALDPESEAIFLNNLAGLTAGRTVIIVSHRLSALTRADA
                     IMVLDRGHIVDAGRHEELLGRCAVYTQLWNQQTRHL"
     gene            complement(11510..15763)
                     /locus_tag="PLH85_13040"
     CDS             complement(11510..15763)
                     /locus_tag="PLH85_13040"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNB65538.1"
                     /translation="MTLAIDTILSIASATPSLLEGNGASTAFEFTVTRSGSATGAVSA
                     TWTVGGAAIGAEDFVGGVLPGGVVNFADGETTKTITVNVAGDLGVEADEAFFVKLTGA
                     TGALIGTDTASAVILNDDTALGIAALTGAQAEGNSGSTPFTFTVTRSGNLSGSASAAW
                     AVSGGSANATDFVGGVLPTGTVTFAAGESSKTVTVNVVGDTVGEADETFNVTLANVTG
                     ATVTTATASATILNDDALLNIATVTASAAEGSSGSTPFGFTITRSGNTAGVSSVKWAV
                     SGGSAVAADFSGSVLPSGTVSFAAGETTKTITVNVAGDTALEADESFNVVLSAASGAT
                     LGTATASALILNDDAQLAIAAASANMAEGTGASATPFTFTVTRSGYLGAAASANWAVS
                     GGTATAADFTGGVLPTGTVNFAPGESSKTVTVNVAADSLVESNETFNVVLSGATGASL
                     GTTTATGTIVTDDTQLDIKAGVASKAEGNTGSTPLTFTVTRSGITTGASTANWAVSSA
                     TADATDFTGGVLPSGTVSFAAGETSKTLTVNVAGDNLIESDETFAVTLSGATGATLGA
                     ASASTTILNDDATMSIAAADARKAEGSGSTATPFTFTVTRSGSLIGASTVKWAVSGGT
                     ATATDFSGNTLPTGTVSFAAGETSKTITVNVAADSLVEADETFNVVLSTPTGGVIGTG
                     TASGTLLNDDAQLAIAATSASKAEGTGATATPFTFTVSRSGNLAGTSTANWAVSGGTA
                     TAADFSGSVLPTGTVSFAAGESSKLITVNVAADSLVEANETFNVVLSAPSAGTSLGTA
                     SAAGSIVNDDTLVSLATSAVSKVEGNSASTAFTFTVSRTGVTTGTSSVGWAVTGGTAS
                     AADFTGGALPTGTVSFAAGETSKTLTVNVAGDNLIEGDETFTVGLTTATGATIGTATA
                     NATILNDDALVGIAATSAVRSEGNSGSTPFTFTVTRSGTLTAASTVNWAVSGGAVTGA
                     DFTGGALPTGSVSFAAGETAKTITVNVAGDGNFEGDEAFTVSLTGTSGAQVDATAASA
                     TGTILNDDGAVDITADTSRFVEGNVGSSTASFTLTRRINTSNTASINWAVSGGTATTS
                     DFIGGVLPSGTVSFAAGETTKTITVNTVADTLYETDESFNVVLSGGVGTGIGIGSATT
                     VILNDDPVLNIAAINPAQVEGSGGGSTPFAFAVSRGGNLTGTMAVRWAVASPNASGAD
                     FVAGVLPSGTITFADGEALKIINVDVLADDLPENDKSFLVGIAGPFGVSVGKSSATVT
                     LVDDDNYLVTDVASPTVVLQGTMADDVLTGGAGPERIIGLAGKDTLTGGGGADQFLIT
                     APSDGPHTITDFSTADGDRIQILATNFGLIPNSTPVLLSGSTLPTPSTSGGQFLYNAS
                     NGALWFDRDGADSGFAAVQVATLSNRPTLSASDLQLVLA"
BASE COUNT         2338 a         4981 c         6450 g         2665 t
ORIGIN      
        1 gcgaggaggg cgcggacgag ttcgccgacg ggggccgcgg cgaggagggc cgggatgctg
       61 cgcacggcga gttcctgggg accggcgggg ccgatttcga agcccatgcg ggccaggagc
      121 tcggcgtgtt cctcggcgct ggccatgtcc tgggcgccga cgccgaagac tgcggggatg
      181 agcaggcgct ggacgccggg ggtgccgtcg aggagggtct tgaggcgttc gtagaggatg
      241 cgttcgtggg cggcgtgcat gtctacgagg atcaggccgg cttcgttctg ggcgaggatg
      301 tagacgccgt ggagctgggc gagggcgtag ccgaggggtg gggcgaggtc gctggcgggg
      361 aggggagcgt gcggggtctc cggaccgttg ccaacggcgc cggcagaaac gccgggtgac
      421 gcgccgacgg gtgcagggcc cgccggccgg gctgcggcgg cgaagtcgaa ataggcgcgg
      481 gagaccggct ccatggcgag gcgaccttgc tggggtgccg gcgcataggg tgcgccgcct
      541 gcgtggctgg ggaccgggcg gggcgtgatt cccgcaccgt cgcggtcggg ttcacccgtc
      601 tgggcggcgc cggaggcgga gagggcgcgg gtgagggcgt ggaagacgaa ctggtgcacg
      661 gcgcgggcgt cgcggaagcg cacttcggtc ttggcgggat ggacgttcac gtccacgccg
      721 aaggggtcga gttccaggaa cagcacgaag gccgggtggc ggctgccgtg gaggatgtcg
      781 gcgtaggcct cgcggatggc gtgggcgatc agcttgtcgc gcacgaagcg accgttcacg
      841 tagaagaact gggcgtcgcg gctgctgcgg gcgtaggcgg gcagggaggc gtagccgtgc
      901 aggcggagca gcccggaggc ggcttccacg gcacgggcgg cggccaggaa gtcgtcgccg
      961 aggagggcgc ggacgcgggc ctcacgcggg ccggcgggca gacgcagacc gacgcggccg
     1021 ttgtgggtga gctgcagggc cacgtccggg cgggccaggg cgacgcggcg gaagacgtcg
     1081 tcgcagtggg caaattcggt gccttcgctc ttgaggaatt tgcgccgggc gggggtgttg
     1141 aagtagaggt cggcgacatc cacgacggtg ccgtggttga gggcggcagg ggcgagttcg
     1201 cgcgggtggg cgtcgatgcg ccaggcgtgg ggggcgccgt cggcgcggct ggtgacgctc
     1261 aaacgcgaga cggcggcgat ggcggccagg gcttcgcctc ggaagcccat ggtgccgacc
     1321 cgctccagat cgtccaggct ggcgatcttg ctggtggcgt ggcgctccag ggcgaggggc
     1381 agttcgtcct tggggatgcc gccgccgtcg tcggccaccc ggatgcggcg cacgcccccc
     1441 tggtcgagtt gcacgtcgag ggcggtggca ccggcgtcga gggcgttctc gaggacttcc
     1501 ttgaggacgg aggcgggacg ttccaccacc tcgccagcgg cgatctgatt gatgaggtgg
     1561 tcgggcagaa cctggatggg catcgggcgt ccggtgaaac gatggagggg cggggcgcat
     1621 tgtaatggct cgcgacgggc ccgtccgggg cataatccgg cctgcatccg gtttgtgaca
     1681 ttcgatcatg caactgacct acatccgccc tcaggggcgg ctcgccgagg tggtggcggg
     1741 ctacctcttc gcttcgacgc tggacgagtc gtggccgacc gacatgcctt ggctgtcctc
     1801 cattccagca ggtaacgaat gggcgttgac ggtgttcacc gagggacgtg cggtgctgtt
     1861 gggggacggt ctggcgccgc cgcaggtgct gccgtcgtgg gtgctcagcg gaccgcggtt
     1921 gcgccccgtc acgctgatga acctgtgccc gttacgggcg gtgtcggtgc tcttccacgg
     1981 ggatgccctg gcgcggctgg tgaacctggc gccggtgcat ctgccgcagg ggtgcgtcga
     2041 tccggcggag ctgctgggcg cggactgggc cttgttccgc tcggccctgg gcgctcgcgc
     2101 gggagaccgg gtggcccagc agaactggct ggaggctttt ctcgagcccc gctgggaggc
     2161 gcgccgtctg gggttgtccc gtgcgccgac ggcgaccgtg cgggcggccg gacggatcgc
     2221 cgcagcggcc tcggcgcggg cgatggggtg gcacgagcgc tgcatcgagc ggagcggtcc
     2281 acgggcgatc gcggtcactc cggccgtggg ccggcacgtg gcgctcatcc acggggcgct
     2341 gatggaagcc tggggattgg gaccggcggc gggggcccgg cgcctgggtg acatggcgcg
     2401 ggcgctgggg gtggcgcatc ccgctggcct ggtccgggcg ttggcggccc ggacctgggt
     2461 gggctcggag cgggcgacgt cggcggagtc ggcgtcgccg cccgaagcac tcagaagccc
     2521 agtacgtcca gccgggtgat ggcccggccg tccttgctga cggtgccggg cgcgacggcc
     2581 gcctgcttga tggcggcctc cacggcggcc ggcgtggcgt ttggattgcg ggacaggtag
     2641 agggccgcgg caccggtcac gtggggcgtg gccatggagg tgccggacat ggtcgccatg
     2701 ccggcgttga ggtaggtgga gaggatggag acgcctggcg cccaggtgtc cacgcagctg
     2761 ccgtagttgc tgaaggaggc ttccttgttg gcgctgtcgg tggcggcggt ggtgatggcg
     2821 ccggccaggc tgccacccac gcgggccggc gaatagttgc aggcgttggc ggcgctgttg
     2881 ccggccgcaa tcgcgaagac gatgcccgcg ttcaccgcct tggtgacggc cgcatccagc
     2941 gtggtgctgg cgctgccgcc caggctcatg ttggccaccg ccggcttgac ggcgttggcg
     3001 gtgacccagt caatgccctt gatcacggtg gaggtgctgc cggaacccgt gcagtcgagc
     3061 accttgacgc cggtgagcgc caccgcgggc gccatgccca ccacgtcggc ggtgttgtcg
     3121 cgggcgccga tggtgcccga cacgtgggtg ccgtggccgt tgcagtcggt gttcttgccg
     3181 tcgccggtga agttcacgtg gttgatcttg ttcaactcgc catggggtgc gatccccgtg
     3241 tcgatcacgt acacattgac ggtgctgacg gtgcccgagc cgtctcccgc cagggtggag
     3301 gacagggtgg cgcgggtggt ggagatgccc cacggaatgg tctgggccgt cgtatgcatg
     3361 ggcaggtcct cttcaatgct gaggacggtc gggtcggcct tgaggcgggc cacctgggca
     3421 tcgctgaggg cggccgcgaa gcccttgatg acgctgctgt aggcgtggag agcctggatg
     3481 ccgtggcggc tttcggtgct ctgcacgcgc tcgatcacgc ggctatccac gtagccccag
     3541 ccgcggggat cttcgtcggc gcgtcggtcg tggaccggac cgcggggtgg ggcctgcacg
     3601 gcggtgtcct ggaactggac gatgtagcgc ttgggggtgt cggcgtgggc cgtggaagtg
     3661 gcgacgacgg cgagcgcgcc ggttagtagt gacgcgagtg acgcgagtgg ggagaggcgc
     3721 atggtgggct ccatgatcgg gaccgctgcg ggtgaggcgg ccggtccggg ttgaaaagga
     3781 aagttgacta tacatgggaa aatttcccat atatagtctc cctatggaaa tgcctcccta
     3841 cccaaggtcg gtggcgcccg ccgggcgggc ggtgctggat cacgccctgg tcatcctgcg
     3901 tccgctggtg gcgtggctgc tgcgctcggg cgtgggctac ggggaattcg cagcagcgct
     3961 caagccggtg ttcctggaca tggccctggc cgaggtggcg cggggcggca ccaagctcac
     4021 ggattcggcc ctcagcctgt tgtccggtct tcatcgcaaa gacgtgcggg ccctcgtgcc
     4081 ggtgctgcag ggcgccgccg acccgtccgc ggccctcggc aaaccgtcgc ctgccagtca
     4141 ggtggccacg cgctggctca ccaacggctg gccggagcgc ctgccggtgg cgggtccgga
     4201 gccgtccttc gaagcgctgg cgcgttcggt gtcccaggat ttccaccctc gcgcggtgct
     4261 gctggagctg atccgcctgg gggtggtcga ggaggcggac ggggcggtgc atctgcgtca
     4321 ggcggctttc gtgcccgatg cccgccacga ggagacgcgg cgcctgatgg ctggcagcgt
     4381 ggcggatcac ctggcggccg gcgtgcataa cctcaccgag gacggttcgc gccgcttcct
     4441 ggagcagagc gtctttgccg acgggctgtc ggcggagtcg gcgcgggccc tggagcagct
     4501 tgccaacgcc ctgtggcagg acgtgctgac ggccatggtg caggccgccg tgcccctgtg
     4561 cgagaaggat gaagcggcgg ggggcgatca gcgcatccgc ctgggcatgt tctgttattc
     4621 ggcccccatg gccggcgctg ccgcgcccga cgcggcggcg gacgaaaagg atcctcccgt
     4681 atgaagaccc gatttctagc gccggtgtcg gggcgtctgg cccgttgggc cgcccgcccg
     4741 cgcctgtctg ccgcgatgtt tctcatcgca ttgctgagtg cctgtggcgg cggcggggca
     4801 ttcgatgtgg ccggtgtggg cagcggcggc agcggggtgg cttccgggac ggtgacgggc
     4861 ttcggctcgg tgatcgtgga cggtgtggaa tacgacgaca gcagcgccag ccggcaaagc
     4921 gaggacgcga cgggcagtct ggccaatgcg ccggtcaagc tcggccagca ggttcagctg
     4981 aactattccg gcagtcgggt ggcccagcgc atcgaggtga tcgcccagtt gctggggccg
     5041 gtgacggccg cgccgggcag cgacgggcgg cttgcggtga tggggcagcc ggtgcgcgtg
     5101 gtgacgagcg tcagcgatgc cagccagagc acgccgacgg tgctcgacgg ctatgcggcg
     5161 gtgacggcga tcgcagtggg cgacgaggtg gaagtgcatg gcgcctgggt gtacgacagt
     5221 gccgtggcga gcaatgtgct ggtggcgaca cgcgtcgaga agcgcagcgt cgcggcggat
     5281 ccggtgcaac tgggcggggt ggtgacggcc gtaggcagcg ggacgctgcg cctcaactcg
     5341 gcgaccggca cccaggtgag cgggtcggat ctggcggaga tcgcggtggg ccaggtggtg
     5401 cgggtgtggg cggcgcgcag tgcgctggcg gcgtcgccgg tggtggcacg gcgtgttgcg
     5461 gtgcagggtg ttacggcgtc ggagctgggc acggccggcg gaaccctgag cggcctcgct
     5521 tcccagtacg atccggccag ccgcagcgtg agcatccagg gcgtgcgcgt gaagctgccg
     5581 gacagcatga cggtggacag tgccgccctg gcccgtggtg agttcgtgac cgttcaggtg
     5641 acggcgtcgg gttcgacgct ggtggcaagc accgtggccc agcgcagcgg atcggctggg
     5701 gcggatctgg gccgcaccgt ggaggtgaag ggcgtcaccc gcggcatcga ctggagcgcg
     5761 acggcggtga gcttcaccct gcgggacacg cccatcgtgg ccgccgccgc cgcgatcgac
     5821 gcgtcctgcc ggtcggtgag tccgacggcc agcgtctatg tggaggtgag gggagccctg
     5881 gcccagtcgg cggcgacggt gacggcgaat tccgtgaagt gcagccagag cgtgcccggt
     5941 tcggcgacgg tcgattacac cgggacgatt gcggccatcg acgcaagcgc ccggaccctc
     6001 acgctgcgcg tcgatgtttc aggtgccagc gtgagcggga cctgggacgc ccaaacctat
     6061 ttcgaacaac gccccgccag tctgccggtc ggccttcggg tcgaggttga gggtgtgatg
     6121 gatcaaggca gcagcacgtt gcgactcagc aaggtccgcc tggaggattg aagcgccggg
     6181 cgggtcggcc catttccgac atgaaccgac attgaccgac atgaggagtc gagcatgaat
     6241 ctattcttta acctgccccg ccgggagcgg gtcctgggcg tcgtgcccgt ggcgctggcc
     6301 ctggcgctgg cgggctgcgg cggtggcggc ggcacgtcgt ccgcgtccgc gtcatccagc
     6361 agcaccgcca ccgaatcttc cagtggcacc gtgacgggct tcggcagcgt gttcgtcgac
     6421 ggcgtcgagc tggaggacgc caaagccgtg acccgcgtgg agaacgcgga tggcagctac
     6481 agcctgacca gcacgcaact ggggcagcgc gtgcgcgtgg cccacgatgc caccggtacg
     6541 gcgtccaccg tcactgtgga tgccgccgtg atcggcacgg tagcgagtgg gagcgtcgac
     6601 gcgacggcgc tcacctttac ggtggccggc cagtcggtca aggccaattc cgatagcacg
     6661 tccggcccgc tgacggtgta tggcggcggc tacacggcct tttccgacat tgccgccggg
     6721 gacctggtgg aaatccatgg cagcgcggtg tacgacagca cggccaaggt gtacgtggta
     6781 caggccaccc gcatcgagaa gaaatccgcc atctcggcgg tgcgcgtgat gggcaagatc
     6841 gccagcctgg acaccacggc gaagaccttt gccatcaaca gcctgacggt ggcctatggc
     6901 tccgccatcc tggcgcccac caccgccacc cttgcggatg gcgtcaccgt ggtggcctgg
     6961 ggcgcccaga actccctggt gagcggcagt tcggggctga gcctgaccgc ctcgcgggtg
     7021 cgcgtgctca atgccggtct ggctgacgac agcgccagtg gcgcgaccca gctcggcggc
     7081 gtagtgagca actacagcgt cagcgccgga acctttgagc tggaaggcgt gaaggtgatc
     7141 gtcggtagcg cgactctcag cccgaccggc gcgacgatcg ccaacggcgc ctacgtgcag
     7201 gtgagcggga ccgtcggcag tgacgcgtcc atcaccgcga cgagcatcaa agtgcgccag
     7261 cagagcacca ccgacgacac cgccagcgtg cgcctgatcg gcgccatcga aagcctgtcg
     7321 gatcagacgt ccttcgtggt gcgcggcgtg ccggtggacg cctcggcgat caccctgagc
     7381 acggcctgcc cgggcgtgac cctggcggtg ggcacggtgg tgaaggtgac ggcgacggtg
     7441 cagagcggca ccgatgtggt caaggccacc agcctggggt gcacctcggc gacgacctat
     7501 acgatgctga gcgccagcgg cacggcgggc acggtgaata cgacggtcag gaccttcgtg
     7561 ctcaccacga ccggcaccgc caccacgacc cgtacggtgc agtggagcga ccagacggtg
     7621 tttgccggcg tcacggccgc gacgctggac ggcgttacgg tggccgtgga gggctatctg
     7681 gacagcagcg gcgtgctcgt cgcccgcaag atccgcaatc ccgccgtggc tgcaggccgt
     7741 gaggacgctg atgcctatgc gggcgtggat gacggctcgg ggcgttcgtg gagccgctat
     7801 cgcagcacgc acaggtgatc tgaaccgcgt ggaccggtcc gcaggagacc ggcgattcgc
     7861 gcgaccgctg tcgcaacttg gccgggcacg tgtgcccggt ttttttgcgt ggcgggagct
     7921 gcggcgatca gcgttcccgc agggattcgt cgagggtgcg gatcagcgga tagaggaaga
     7981 aggcgatcac cgagcgtcgg ccgacgacga tctcgccggt gaggctcatg cccggcagca
     8041 gcagcgtcgg ctgggcgatc ccgtccaggc gggtgtcgtc cagggcgacc cgggccaggt
     8101 agtaggacgt cgtggcgccc ccggggccct ggcgcgaaaa ggcgtcggga ctcacgtgca
     8161 gcacccgccc gcgggccgtg ccgtgcttct ggaacgggta ggtgtcgaac ttgatgcgca
     8221 cggtgtcgcc cggccggatc aggcccacgt cgccggggcc gatctccact tcggcctcca
     8281 ggggcgcgtt cattgggacc agggtgaaca ggggctcggc ctccttgagg acggagccga
     8341 ccgaacgttt gccgatttcc agcaccaccc cgtcctgggg cgccgtgagg cggatcaggt
     8401 cggtgcgcag gcgggtcttg tccagctggg cggtgacctc gtcccgctcc tggacagtct
     8461 gggagagctt ttccagggaa tcctggcgcc aggtacgcag gtagccggta cgctcggcct
     8521 cggccgccgc gatctcgcgg cgcagttcct ggtcccggtg ggcggcgatg ttgcggtcgc
     8581 gttccacctc cagcttgcgt tcgcgcagtt ccagaacctt ggcgcgggag ccgaactggc
     8641 gggcctccag ctcggcctgc atggcttcca gctcggccag actgtggacg cgctcagcca
     8701 gggtggcttc gtcgcggcga ttcgtctcta gcgctgcacg caggcgggca atgctttcgt
     8761 cgtactgctt gaggcgcgcg gcgtgggcgg cctggcgctc tgccatcagg cgggcctgaa
     8821 cgccttcctg gcccgccgca gtgctggggg tcgccgggtt gccgtggagt tccgcctcca
     8881 ggcgctgcgc ccgctgggcg aggctgtcgc ggcgggcgcc gagctgaccg gcgtcggcct
     8941 gggcgaaggt cgggtcgagg gtggcgagca ccgtaccctg tcgcacctgc tgaccgacgc
     9001 gcacgtcgat gctcttgagg atgccggcct ccaggggttg caccaccaga ttgggcaggg
     9061 gcgtgaccag gcggccgcga ccgatggcca ggatgtcgat ctcggagacg ctggcccaca
     9121 ccagtgccgt gaccaccagg gcgagcaggg catagaggag gacgcgcgga ccgtggggtg
     9181 gttcggcgtg ttccagcgcg tggacctccg gcaggaagtc ggtgagggcg gcgcggaggg
     9241 tgcggcggtc catggtcaca ggtgccgggt ctgctggttc cagagctggg tatacaccgc
     9301 gcagcggccc agcagttcct cgtggcgacc ggcatccacg atgtggcccc ggtcgaggac
     9361 catgatcgcg tcggcgcggg tgagggcgga caggcggtgg gagacgatga tcaccgtgcg
     9421 gcccgcggtg agtccggcca gattgttcag gaagatggcc tcggattccg ggtcgagggc
     9481 gcttgcagcc tcgtcaagga tcaggatgcg cggccggttg aggagggcgc gggcgatggc
     9541 caggcgctgg cgctggccgc cggagaggtt ggcggcgttc tcttccagca gggtgtcgta
     9601 gccctggggc agacgttcga tgaactcgtc ggcgccggcc aggcgggcgg cggcgacgat
     9661 ctcgtcgaag ccggcagtgg gccgcgtgat ggacaggttg tcgcgcaccg tgccgcggaa
     9721 caggaagttg tcctgcagca ccacgccgat ctggcggcgc aggtgggcga ggtcgatctc
     9781 gcggaggtcg atgccgtcga agcggacgat gccgtcctgc accggataga ggccctgcat
     9841 caggcgggtg agggtggtct tgcccgaccc gctgcggccg acgacgccca ccaccttgcc
     9901 ggcgggcagg ctgaagctga tctgctccag ggccgccgcg gcacccgggg cgtaacggaa
     9961 gctgacgttt tcgaaggcga tgtgcccctc cagggtgggc tccaggccgc cggaggcccg
    10021 ctggccttcg accggacggt tcatgatctc gccgagcatg cgcacggaca gggcggtctc
    10081 ctggtattcg tggaccagcg ccacgatctg aacgaggggc gacacgaccc gcccggagag
    10141 catctggaag gcgatcaggg acccgacgct gagggtctgg tcgaagacca ggttggcgcc
    10201 cacggcgatg acgctgacgc ccatcagctt ttcgagcagg ctcatggccg tgtgggcggc
    10261 cagggagatg cggcccacgc ggaagtgcag cgccaccgtg cgcgcggagc gggcatccca
    10321 gtcgtcgcgg cgcaggggct ctaccgccag ggccttgacg gtgcgcatgc cctggatggt
    10381 ttccacgagc agcgactggc gttccgcctc ggccgaatac agggcctgca ggcgggcacg
    10441 gaaggccggg atcagggcca ccacgacggc gccgatggcg gcggcgaagg ccagtaccag
    10501 ggtggccagc agtccggagt agaagaacat gaccggcagc agcaacacca gcaccgaggc
    10561 gtcgagcagg gtcgagaaga ggcggccggt gaggaactgg cggatctttt cggcctgctg
    10621 catgtggcgg gtgatgacgc cggcggtgcc cgtctcgaag aaggtgatgg gcaggcgcag
    10681 gagatgggcg aaggtcttgc gggccagccg gacgtccagc cggttggtgg cctggagcat
    10741 gaaggtctga cgcaggtagg agaaggccgc ctcgaacagc aaggcgagga ttacgccggc
    10801 cgtcagcacg tagagcgtgg agtagccctg gtgcacgatc accttgtcga tgaccagttg
    10861 ggtgaacacg ggcatggcca gggccagcac gtgcaggacc agggcggcga cggccacgtc
    10921 ccgcaggaac gccttctggc ggaggagctc cgggacgaac cagcccagtc cgaagggccg
    10981 gttctcgtca tggaggctgt acacgcgcgt gaccagcacg agatcgccgg accaggcggc
    11041 ctcgaagcgc gcccgatcga tcaggaaaac cgcgtccgcc ggggtggcca acgggtcgag
    11101 cacggccacc gcggcctcct cgccctgccc gcggagtccg accagcacga cccagttgcc
    11161 attgttgagg cgggcgaggg ccggaaaggt gtcctgcagg ggcgggagcg ccgggaagtc
    11221 ggtcttcagg ctgcgcgcct tgagcccgat ggcgcgggcc gccgccacca gcgtcgggag
    11281 cgcgtcggcc ggcgtgccgg ccgccgccgg atgggcgcgc cggatctgct cgggagaggt
    11341 gttgacgcgg tggtgccggg ccaccgcggt gaggctcctg agtgctgtct caaagggcgt
    11401 gagggaatcc atcgatggac ggtgtcccgt gatggtcagc agggaacgag ggccggcaag
    11461 cagggctgga ccgggcccga gggcctggtc ctgtcccgca ggaggatgct caggccagca
    11521 cgagctggag gtcgctggcg ctcagggtgg gccggttgct gagggtggcc acctggaccg
    11581 cggcaaagcc gctgtcggcg ccatcgcggt cgaaccacag cgcgccattg ctcgcgttat
    11641 acaggaactg gccgccgctg gtgctgggcg tcggcagggt ggagccgctc aacaggacgg
    11701 gcgtggaatt gggaatgagg ccgaagttgg tggcgaggat ctggatcctg tcgccgtcag
    11761 ccgtggagaa gtcggtgatc gtgtgcggac catcgctcgg cgcggtgatc aggaactggt
    11821 ccgcgccgcc gccgccggtc agggtgtcct tgcccgccag gccgatgatc cgttcgggtc
    11881 cggcgccgcc ggtcagcaca tcgtcggcca tggtcccctg gaggaccacg gtgggggacg
    11941 ccacgtcggt caccaggtag ttgtcgtcgt ccacgagggt caccgtcgcc gaactcttgc
    12001 cgaccgacac gccgaagggg ccggcgatgc cgaccaggaa ggacttgtcg ttctccggga
    12061 ggtcgtcggc gagcacatcg acgttgatga tcttgagcgc ttcgccgtcg gcaaaggtga
    12121 tggtgccgct cggcaggacg ccggcgacga aatcggcacc ggaggcgttc gggctggcca
    12181 ccgcccagcg tacggccatg gtgccggtga ggttcccgcc gcgcgagacg gcgaaggcga
    12241 agggggtgga accgcctccg gagccttcga cctgggccgg gttgatcgcc gcgatgttga
    12301 ggaccggatc gtcgttcagg atgaccgtcg tggcggagcc gatgccgatc ccggtgccca
    12361 cgccgccgga cagcacgaca ttgaaggatt cgtcggtttc gtagagggta tcggccaccg
    12421 tgttgacggt gatggtcttg gtggtctcgc cggcggcgaa ggagaccgtg ccggagggca
    12481 ggacgccgcc gatgaagtcg gaggtggtgg ccgtgccgcc ggagacggcc cagttgatgc
    12541 tggcggtgtt gcttgtgttg atgcggcgcg tcagggtgaa ggaagcggtc gagctgccca
    12601 cgttcccctc cacgaagcgg ctcgtgtcgg cagtgatgtc cacggcgccg tcgtcattga
    12661 ggatcgtgcc ggtggcggat gcggcagtcg cgtccacctg ggcgccgctg gtgccggtca
    12721 ggctcacggt gaaggcttcg tcgccttcga aatttccatc gcccgcaacg ttcacggtga
    12781 tcgtcttggc cgtctcgccg gccgcgaagc tcacgctgcc ggtcggcagc gccccgcccg
    12841 tgaaatcggc gcccgtgacg gcgccgcccg agaccgccca attgacggtg gaggcggcgg
    12901 tgagggtgcc gctgcgggtg acggtgaagg tgaagggcgt gctgccgctg ttgccttcgc
    12961 tgcgcacggc actcgtcgcg gcgatcccga ccagggcgtc gtcgttgagg atcgtggcgt
    13021 tggcagtggc ggtgccgatg gtcgcgccgg tggcggtggt cagaccgacg gtgaaggttt
    13081 cgtcgccttc gatgaggttg tcgcccgcca cattgacggt gagcgtcttg gaggtctcgc
    13141 ccgcggcaaa gctgacagtg ccggtcggca gcgcgccgcc ggtgaagtcg gccgcagaag
    13201 cggtgccgcc ggtgaccgcc cagcctaccg aggacgtgcc cgtggtgacg ccggtgcggg
    13261 agaccgtgaa ggtgaaggcc gtgctggcgc tgttgccttc gaccttgctg accgcactgg
    13321 tggcgaggga aaccagggtg tcgtcattga cgatggagcc tgcggcgctg gccgtaccca
    13381 ggctggttcc ggccgacggc gccgacagca ccacgttgaa ggtttcgttg gcttccacga
    13441 ggctgtctgc agccacgttt accgtgatga gcttgctgct ctcgccggcc gcgaagctca
    13501 ccgtgccggt ggggagcacg ctgccgctga agtccgccgc cgtggcggtg ccgccggaga
    13561 cggcccagtt ggccgtggag gtgccggcca ggttgccgct gcggctcacg gtgaaggtga
    13621 acggcgtggc ggtggcgccc gtgccttcgg ccttgctggc ggatgtggcg gcgatggcga
    13681 gctgggcgtc gtcgttgagg agcgtgccgc tcgcggtgcc cgtgccgatg acgccgccgg
    13741 tcggtgtgga caggaccacg ttgaaggtct cgtccgcttc caccagcgaa tctgcggcga
    13801 cattgaccgt gatggtcttg gaggtttcgc cggcggcgaa gctgacagta ccggtcggca
    13861 gggtgttgcc ggagaagtcg gtggccgtgg ccgttccgcc gctgacggcc cacttgaccg
    13921 tcgaggcgcc aatgagcgag ccgctgcggg tgacggtgaa ggtgaatggg gtggcggtgc
    13981 tgccgctgcc ctcggccttg cgtgcgtcgg cggcggcgat gctcatcgtc gcgtcgtcat
    14041 tgaggatcgt ggtgctggcg ctggccgcgc ccagggtcgc acccgtcgcg ccggagaggg
    14101 tgaccgcgaa ggtctcgtcg gattcgatga gattgtcgcc cgccacattg acggtgaggg
    14161 tcttggaggt ttcgcccgcg gcaaagctga cggttccgct cggcagcacg ccaccggtga
    14221 agtcggtggc atcggccgtg gcgctggaca cggcccagtt ggccgtcgat gcgccggtgg
    14281 tgatgccgct gcgggtgacg gtgaaggtga gcggggtgct gcccgtattg ccttcggcct
    14341 tgctggctac accggccttg atgtcgagct gggtgtcgtc agtgacgatg gtgccggtgg
    14401 ccgtggtggt gcccaggctg gcgcccgtgg cgccggacag caccacgttg aaggtctcgt
    14461 tggactccac caggctgtcg gcggccacgt ttacggtgac ggtcttgctg ctctcgccgg
    14521 gggcgaaatt caccgtgccg gtgggcagga cgccaccggt gaaatccgca gcggtggcgg
    14581 tgccgcctga cacggcccag ttcgccgagg ctgcggcgcc caggtagccg ctgcgggtga
    14641 cggtgaaggt gaagggcgtc gccgacgcgc cggtaccctc cgccatgttg gccgaagcgg
    14701 cggcgatggc gagctgggcg tcgtcgttga ggatgagggc gctggccgtc gcggttccga
    14761 gcgttgcgcc gctggccgcc gagagcacca cgttgaagct ttcgtcggct tccagggcgg
    14821 tgtcgccggc cacgttgacg gtgatcgtct tggttgtttc gccggcggca aagctgacgg
    14881 tgccgctcgg caggacgctg ccgctgaagt cggccgccac ggcgctgcca ccggaaacgg
    14941 cccacttgac cgacgagaca ccggcggtgt tgccgctgcg ggtgatcgtg aagccgaacg
    15001 gggtgctgcc ggaactgccc tcggcggccg aggcggtgac ggtggcgatg ttcaggaggg
    15061 cgtcgtcgtt gaggatcgtg gcactggctg tcgcggtggt cacggtggcg ccggtgacgt
    15121 tggccagcgt gacgttgaag gtttcgtcgg cctcgccgac cgtgtcgccg acgacgttga
    15181 cggtgactgt cttgctgctc tcgcccgccg caaaggtgac cgtaccggtc ggcaacacgc
    15241 cgccgacgaa gtctgttgcg ttggcgctgc cgccgctgac ggcccaggct gcactggcgc
    15301 tgccgctcag gttgccgctg cgggttacgg tgaaggtgaa gggcgtgctg ccgctgttgc
    15361 cttcggcctg cgcgcccgtc agcgccgcga tgcccagggc ggtgtcgtcg ttgagaatga
    15421 ccgccgaggc ggtgtcggtg ccgatgagcg cgccggtggc gcccgtgagc ttcacgaaga
    15481 atgcttcgtc cgcctcgaca cccagatcac cggcgacgtt cacggtgatt gtcttggtgg
    15541 tctcgccgtc ggcgaaattg acgacgccgc cgggcaggac gccgccgacg aaatcttccg
    15601 caccgatcgc cgctccgcca accgtccagg tggcgctgac ggcccccgtg gcactgccgc
    15661 tgcgggtcac cgtgaattcg aacgctgtgc tggcgccatt gccttcaagc aggctgggcg
    15721 ttgcggaggc aatactcagg atggtgtcga tggcgagggt catggtggtc agatcagggg
    15781 acggtggacg aaaaaaggtg tatgcggaaa gcaaacaagt gtaaatcccg acatgcctgc
    15841 aaggcggatg tctccgattg cggttcgtga cgtaattcct ccttggccgg attcggaaaa
    15901 atccggaatc cggatgcgcc ccgaccgcaa gccggttgat gcaggctcca gtgggatcgc
    15961 gtgccgaagg cgcccgtgcg gcccgcctga atcggcgcgg gggctgggtt tcagttcatg
    16021 acattcatgt atttgtcaat ggggttgatg ttaaattgtg aatcttacag catcgtttta
    16081 cgcgaattgc gtcggtagaa gcttgatgtg tatcagattg ttctgaattt acgcggggcg
    16141 ggcggcactc ccccggaggc cgacgtgggg cgcgcgggcc gtggctggac gggctcgggc
    16201 ctcggggcgg gcggtcgtgc gcaacgcgtg cggcaatccc cgggcggttg attccagggg
    16261 ggtgcgccag gaaggggcgg gcgtcacgtc gatgcgccgg agatgccggg ctggggtcgt
    16321 gatgtgcgca gcgcgcgcgt gatgcgggag aggagaatcg cctgggaggc gatcggcggc
    16381 ggggcgtgag ggggaggcgt gcggcgtccc gccatcgatc aacgatgacg ggag
//
LOCUS       DAOQMM010000087        16067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1421_length_16067_cov_51.605796, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000087 DAOQMM010000000
VERSION     DAOQMM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16067
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1421_length_16067_cov_51.605796"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..756
                     /locus_tag="PLH85_13045"
     CDS             139..756
                     /locus_tag="PLH85_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HNB65539.1"
                     /translation="MCANFRPSSREALSAFPLAPPDFAYGEAYPGSIVPVVTTFAPRL
                     WVPATFGLVPAWAKDARIQRSTYNARSETVGEKPSFRSAWTRGQLCIIPAAAIFEPCY
                     ESGRAVRWRVERIDGKPMGIAGIWERRLHGDGRPSWSMAMLTINADDEPLFQRFHKPG
                     DEKRAVVILPDEAWGDWLRCKSAVAAQALLQPFDAAALVACPDPR"
     gene            928..1947
                     /locus_tag="PLH85_13050"
     CDS             928..1947
                     /locus_tag="PLH85_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNB65540.1"
                     /translation="MLRRSFVLATAALLTAGLAQAQAPGYRAEYRLSTTLGTAFPWGQ
                     AGERWIELVKEKTQGRINIKLYSGNSLVGGDQTREFTAIRQGVIDLSIGSTINWSPQV
                     KELNLFSLPFLMPDYKAMDALTHGEVGKALFEVLEKRDVIPLAWGENGFRELSNSKRP
                     VASPADLKGLKLRVVGSPLYNETFTALGANPTQMSWADAQPALASGAVDGQENPLSVF
                     VAAKLPTVGQKHLTLWHYVADPLIFVVNKNVWNSWTPADREAVRQAAIQAGRENVDKA
                     RKGIAGNDTAVLRQIEASGVTVTQLSPEQRSAFVQATRPVYDKWSRTIGADLVKQAEA
                     AIARR"
     gene            2014..2499
                     /locus_tag="PLH85_13055"
     CDS             2014..2499
                     /locus_tag="PLH85_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNB65541.1"
                     /translation="MSEPDSSTPAGHSPLARVERFLMAASMGVLCLLTMANVLVRYFT
                     DFSFAFTEEISVSLLVIMTLVGASHAFATRHHIAITFFVDRWSALRPAAERLAVLCGL
                     AMFGLLAWYGTRMAWDDYSFEVTSPSLGVPQWWYTVWLPVLSVLIVVRLALLCFRRTP
                     R"
     gene            2496..3779
                     /locus_tag="PLH85_13060"
     CDS             2496..3779
                     /locus_tag="PLH85_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNB65542.1"
                     /translation="MMDALLFGLFALLMFAGVPLAVAMGLAGVAVVAVADLGMLSLPT
                     NVYTGIAKYPLMAIPVFVIAGLIFERAGVAATIVRFASALVGQRRGSLAIVAILVAMI
                     LGGISGSGPADSAAVGAVMLPSMLKAGYPRAFTAGVIAAAGSTDILIPPSIAFVIYSL
                     LVPQASVPALFAAGMIPGILSGLTLVLVAWWLSVRHGFEANVADERPPFWQSLKDAGW
                     GLLAPVIILGGMRSGLFTPTEAAVVAVFYGVVVGVLIYRSLGWRDIVEVLVESAEISS
                     VILTVVALASVFAWASNTLGTFDHLAAALLGTGLSEIPMLLSIQLLLLIAGMFLDAIS
                     IYFIFLPLLVPIAQHFGWDLVWFGVIITMNMALGQFHPPLGVNLMVTCRMAGVSMEST
                     VPWVLWMIAAMAGTMFLVTFIPDLALWLPRHLGYL"
     gene            complement(3818..4534)
                     /locus_tag="PLH85_13065"
     CDS             complement(3818..4534)
                     /locus_tag="PLH85_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65543.1"
                     /translation="MSARRFSPPRAILVALPLSLMLLACGGGGGGTSVPVASTASFNL
                     QAVQAAEFTTAAARPFTLSGTASGYAVTGSGTEVAGSVTAGTFEGLPALQRTTTVTGS
                     VTVNGVTTPLTTSTVDWSDTHYVPVGAVGGEYVVVQGTPTLPSAAHVGDAGLVATANR
                     YADSSKTSLQGTVRTTYGLAADTATTAILSLTQTYYDTSSAQVEVVITQYRIDTSNGF
                     TPLRASDTDYTRNVSLLMTY"
     gene            complement(4662..6785)
                     /locus_tag="PLH85_13070"
     CDS             complement(4662..6785)
                     /locus_tag="PLH85_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65544.1"
                     /translation="MKPLTIRRRLALGFGFVIALGLAVAVNAIYNLYALQQQFGAYEA
                     QMVARERLALRGQAMLGNGIHYFKNTVLRGGDYPAKFAQSMDALERIADEYRALGPVG
                     AEAEAALGEIRAGSVRYRSAVARAMELRAGGASIGDVDKAIAGADKPIGAALATLIDT
                     QSADTRAAREAFDAAVGRTARLIGLLAAGMVVAGALAAWQISRSVVRPLSQAVAISQA
                     VAAGTLPERIDIATRDETGQLLEALQQMVGTLRGFVDAQRENAHQHDLGMIDQQIDAA
                     RFPGVYGDMAHSINALVQAHIAVKMQVVEVVKRYAVGDLSVDMARLPGQKAQITAAMD
                     SVKGNLQRVNAQIQTLVDAAASGDFTARGDAEAFDHAFRAMILSLNRLMDVADTGLGD
                     VSRLLGALAQGDLTQRIDGQYQGTFARLRDDANATVTQLQKLVAGLQQSAQAIATAAG
                     EIAAGNADLSARTEQQAGSLEETASSMEQLSATVRQNADSARRAHDLTRQSCHVVDQG
                     GSTVKRVVATMADIRESSGKIADIIGVIDSIAFQTNILALNAAVEAARAGEQGRGFAV
                     VANEVRQLAQRSAVSAREIKELIAVSVGKVEDGSVLADEAGHVMEEVVEVFGQVTTLV
                     TDISNASREQSVGIEQVSTAVGRMDEVTQQNAALVEEAAAAAQSLDEQAANLVAAVGR
                     FRLPARPASAAPLQPTGASRRVRSA"
     gene            7188..8336
                     /locus_tag="PLH85_13075"
                     /pseudo
     CDS             7188..8336
                     /locus_tag="PLH85_13075"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
     gene            8709..9218
                     /locus_tag="PLH85_13080"
     CDS             8709..9218
                     /locus_tag="PLH85_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65545.1"
                     /translation="MDALGGDVHEQERVVHDAGGRVSDLSRQAADAAGSARDLARASN
                     RIQSVVEVIQQIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEKSRTSAGEI
                     GADISALAGEIGRVALQIEQQAGDVTRLSSLLTDIAGISSRTAGNADHTRNVADRLRQ
                     LTEAQLNQH"
     gene            complement(9276..10070)
                     /gene="phnE"
                     /locus_tag="PLH85_13085"
     CDS             complement(9276..10070)
                     /gene="phnE"
                     /locus_tag="PLH85_13085"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HNB65546.1"
                     /translation="MNVRTPSSPRVAGVTPGGLLAGLAVLAAVAGSFVFLDLPLTAVF
                     TGAAATEALDFAGRFLPPETDAGFVAKVGGAALETLAISLVATLLAAAGGALLALPAS
                     GRHGRPARIVARFVLNLLRSVPELVWATLLVLAAGLGPFAGTLALAVHTTGVLGRLFA
                     EALENAPAAPAEALTGGGAPALASFLYGTLPQVGDQWLAYALYRWEMNIRMAAILGFV
                     GAGGLGGLLYYSLSLFREAEAGTVILAMLVMAIAVDSVSAWLRRAR"
     gene            complement(10067..10888)
                     /locus_tag="PLH85_13090"
     CDS             complement(10067..10888)
                     /locus_tag="PLH85_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphonate ABC transporter permease"
                     /protein_id="HNB65547.1"
                     /translation="MSPTPRDPAALPRLGATVLALALLGPLLHLTDFRPAALFEAGNL
                     RTVGQFLGGFLPPARDPDFLALLARATVETLAMATAGMAVAFLLALPLGLAMARSLSV
                     SRLADARGSRTGALLRALVRGLLTLLRGIPELVWALLFVRVFGLGPAAGVLALGITYG
                     GMLGKVYGEILESTDARPARGLLEAGSGRLAAFSYGLLPLAAPELVSYTVYRWECAVR
                     ASVVMGFVGAGGLGQLMDQSMKMLNGGEAATILASFLLLVVAADALSEWLRSRLA"
     gene            complement(11004..11801)
                     /locus_tag="PLH85_13095"
     CDS             complement(11004..11801)
                     /locus_tag="PLH85_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB65548.1"
                     /translation="MGFSLKGVGLVHANGHRALQGIDLSIADGERVALVGASGAGKTT
                     LLRLLAAARRPTEGCLHLLGADPWSASPAALRRLRARIGLVHQSPPIPPRQRVVTAVL
                     AGRLGHWPLWKALASLVLPADAPGAARELARLDLTDKLYERCDRLSGGQLQRVALARV
                     LYQQPDLFLADEPVSALDPALSELTVALLNEEATRRGVTLVASLHAVDLALRHFPRVV
                     GLQDGRILFDLPSREVSAAQLRTLYAAEGGLPVASICPAGQPSRRCS"
     gene            complement(11854..12735)
                     /locus_tag="PLH85_13100"
     CDS             complement(11854..12735)
                     /locus_tag="PLH85_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB65549.1"
                     /translation="MISVPFPRRVLLRAAAATLLAAAAGLPCAAFAEDVLRVSAIPDE
                     APTELQRKFAPLGAYLEQETGMKVSFVPVTDYAAVVEALATRKIDMAWLGGFTFVQAK
                     LRTHGTAVPLVQRAEDAKFTSKFITAQDGIKSLADLKGKTFAFGAPSSTSGHLMPRYF
                     LGQEGLNPDKDFKTVAFSGAHDATVAFVQAGKVDAGVLNASVWDKLLEQKKVDTAKVR
                     VFATTPPFFDYNWTVRGDLDPKLQARITQAFLKLDPANPAHKEIMALQRASKFIPTKA
                     ENYAGIETAAHAAGLLK"
     assembly_gap    12965..13064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13381..14358
                     /gene="gcvA"
                     /locus_tag="PLH85_13105"
     CDS             13381..14358
                     /gene="gcvA"
                     /locus_tag="PLH85_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HNB65550.1"
                     /translation="MAYRLPPLSSLRAFEAAARHLSFALAARELHVTPAAVSHQIKAL
                     ESYLGVALFERLPRAVVLTAQGRALLPRVQEGFECFAAAIEAAREAGEGGLTVHAPPS
                     FAQRWLVPRLAGFAAAHPEIALRVASDGANIDGVGAPPEDRLADLAAEPDVLAIRFGN
                     GRYPGFRVERVLTPDYIVVGRPDLVAASALRQPADLLAHPLIHDESIPDASVRPSWAE
                     WFRRVGVADADVSRGPRFSSAVLAVEAALAGQGVVLTLAPLVAADIAAGRLRQPFPER
                     LASPYAYYMVTAEAQAERRPVRAFRDWLRAELARTSGAVEGAGSAEKRG"
     gene            14404..>14849
                     /locus_tag="PLH85_13110"
     CDS             14404..>14849
                     /locus_tag="PLH85_13110"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNB65551.1"
                     /translation="MQLFALGLNHHTAPLAIREKVAFRPERLVEALGDFVQRKPVKEA
                     AILSTCNRTEMYFATGEPQSAADWFADYHRVALQELSPYLYTFPQRDAVRHVFRVASG
                     LDSMVLGEPQILGQMKDAVRQAEEAGTLGATMHKLFQRTFSVAKEVR"
     assembly_gap    14850..14949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14950..15105
                     /locus_tag="PLH85_13115"
     CDS             <14950..15105
                     /locus_tag="PLH85_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNB65552.1"
                     /translation="DDPRKVLEALSLGLTNKFLHAPTRFLNEADGDQLGDSAELVQRL
                     FNLHKDR"
     gene            15154..>16067
                     /gene="prfA"
                     /locus_tag="PLH85_13120"
     CDS             15154..>16067
                     /gene="prfA"
                     /locus_tag="PLH85_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNB65553.1"
                     /translation="MKQRIRDKLDTLADRLQELDRLLATPEAAGDMESYRKLTREHAE
                     IGPVVALYGQYTRAVADEAAARELLADPEMKDLAAAELDDARAAQARIEAALQTAMLP
                     RDPNDERNLFLEIRAGTGGDEAALFAGDLLRMYTRYAERQGWRVETISASDSELGGFK
                     EVIVRVSGAGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEVGDVELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGIVAECQDGRSQHQNKASALRVLAA
                     RIKDVQVREQQAREAATRKSLVGSGDRS"
BASE COUNT         2293 a         5287 c         5729 g         2558 t
ORIGIN      
        1 ccaccagata tttcagacaa aaagcccagc cgtgaccggt tgggcttttt gtttgtctgg
       61 gccggttcgt tccccaatgc gtcgccaggt gccttgtgga agccggtgat gggccgacag
      121 acgcgctacg cttgaaccat gtgcgccaat tttcgaccgt cttcccgcga agcgctgagt
      181 gccttcccct tggcgccgcc ggatttcgcc tatggcgaag cctaccctgg cagcatcgta
      241 ccggtcgtca ccacctttgc gccgcgcctg tgggtgccgg cgaccttcgg tctggtgcct
      301 gcgtgggcga aggatgcgcg gatccagcgg tccacgtata acgcgcgctc ggagaccgtg
      361 ggcgagaagc cctcgtttcg cagtgcatgg acgcgcggcc agctctgcat cattccggcc
      421 gccgcgatct ttgagccctg ttacgagtcc ggcagggcgg tgcgctggcg cgtcgagcgg
      481 atcgacggca agccgatggg gatcgccggg atctgggagc gacgcctgca tggcgacgga
      541 cgaccgtcct ggtccatggc catgctgacc atcaacgcgg atgacgagcc gctgttccaa
      601 cgcttccaca agccgggcga cgaaaagcgg gcggtggtga tcctgcccga cgaggcgtgg
      661 ggggactggc tgcgttgcaa gtctgccgtc gcagcccagg ccttgctgca gccgtttgat
      721 gcagcggcgc tcgtcgcgtg ccctgaccca cgttgacgcc ggcattctct gtgtctggcc
      781 cccacgttgc gggtggggcg cggtggcagg acatccgcct ccgccgagct ccgggcgcag
      841 atccccgcac ggcggcggca gcccgcgctt gcccgcccgc atgtgcgacg ccgataatcg
      901 tcctctttcc cttccgagga gttccccatg ctgcgccgat cctttgtcct ggcgactgcc
      961 gcccttctga ccgccggtct ggcccaggcc caggcgccgg ggtatcgggc ggagtatcgc
     1021 ctgtccacca ccctcggcac ggccttcccg tgggggcagg cgggggagcg ctggatcgag
     1081 ctggtcaagg agaagaccca gggccggatc aacatcaagc tgtattccgg caattccctg
     1141 gtgggcgggg accagacgcg ggagttcacg gcgatccgcc agggcgtgat cgacctgtcc
     1201 atcggctcca cgatcaactg gtcgccccag gtcaaggagc tcaacctgtt ctcgctgccc
     1261 ttcctgatgc cggactacaa ggcgatggac gcccttaccc acggcgaggt gggcaaggcc
     1321 ctctttgagg tgctcgaaaa acgtgacgtg atccccctgg cctggggcga aaacggtttc
     1381 cgtgagctat ccaactccaa gcggccggtg gcctcgccgg cagacctgaa ggggctcaag
     1441 ctgcgtgtcg tcggctcgcc cctatacaac gagaccttca ccgccctggg cgccaacccc
     1501 acccagatga gctgggccga cgcccagccg gcgctggcgt ccggcgcggt ggacggccag
     1561 gagaatcccc tgtcggtgtt cgttgccgcc aagttgccca cggtcggtca gaagcacctg
     1621 accctgtggc actacgtcgc agatccgctg atcttcgtgg tgaacaagaa cgtctggaac
     1681 agctggactc cggccgaccg ggaagcggtg cgccaggcgg cgatccaggc cggccgtgag
     1741 aacgtggaca aggcgcgcaa gggcatcgcc ggcaatgaca ccgcggtgct caggcagatc
     1801 gaggcgtccg gcgtcaccgt cacgcagctc tcgccagagc agcgcagcgc cttcgtgcag
     1861 gccacccgcc cggtctatga caagtggtcc cggaccatcg gcgcagacct cgtcaagcag
     1921 gccgaagccg ccatcgccag gcgctgagcg ccgtccgtcc cccttcaatg ccggagcggg
     1981 ccgaccgctc cggtgcttca cggcattttc cccatgtccg aacccgattc ctccaccccc
     2041 gccggccatt cgccgctggc gcgggtcgaa cgcttcctga tggccgcctc catgggcgtg
     2101 ctgtgcctgc tcaccatggc caacgtgctg gtgcgctatt tcaccgactt ctccttcgcc
     2161 ttcaccgagg agatttccgt ctccctgctg gtgatcatga ccctggtggg ggcgtcccac
     2221 gcctttgcca cgcggcacca catcgccatc accttcttcg tggatcgctg gtcggcgctg
     2281 cgtcctgcgg cggagcggct ggccgtgctg tgcgggctgg ccatgttcgg cctgctggcc
     2341 tggtatggca cgcggatggc ttgggatgac tacagcttcg aggtgacgtc gccgtccctt
     2401 ggtgtgcccc agtggtggta cacggtctgg ctgccggtgc tgtcggtgct gatcgtcgtg
     2461 cgtctggccc tgctgtgctt ccggaggacg ccgcgatgat ggacgcgctg ctcttcgggc
     2521 tcttcgccct gctgatgttc gcgggcgtgc ccctggccgt ggcgatgggc ctggccggcg
     2581 tggcggtggt ggcggtggca gatctgggca tgctgtccct gcccaccaac gtctacaccg
     2641 gtatcgccaa gtacccgctg atggcgatcc cggtcttcgt catcgccggg ctcatcttcg
     2701 agcgggccgg cgtggcggcc accatcgtgc gcttcgcatc ggccctggtg gggcagcggc
     2761 gcggcagcct ggccatcgtg gcgatcctgg tggcgatgat cctgggcgga atctcgggct
     2821 ccggtccggc ggattccgcc gcggtgggcg cggtgatgct gccgtccatg ctcaaggccg
     2881 gctatccgcg cgccttcacc gccggggtga tcgccgcggc gggctcgacg gacatcctga
     2941 tcccgccctc catcgccttc gtcatctaca gcctgctggt gccccaggcc tcggtgccgg
     3001 cgctctttgc cgccggcatg atccccggca tcctgtcggg cctcaccctg gtgctggtcg
     3061 cctggtggct gtcggtgcgg catgggttcg aggcgaacgt ggcggacgag cgcccgccct
     3121 tctggcagag cctcaaggac gccggctggg gcctgctggc accggtgatc atcctcggcg
     3181 gcatgcgcag cggcctgttc acgcccaccg aggcggcggt ggtggcggtg ttctacggcg
     3241 tcgtggtcgg tgtgctgatc taccgcagcc tcggctggcg ggacatcgtc gaggtgctgg
     3301 tggagtccgc cgagatctcg tcggtgatcc tcaccgtggt ggccctggcc agcgtctttg
     3361 cgtgggccag caacaccctc ggcaccttcg accacctggc ggcggcgctg ctgggtaccg
     3421 ggctgtcgga gatccccatg ctgctgtcga ttcagctcct gctgctgatc gccggcatgt
     3481 tcctggacgc catctccatc tacttcatct tcctgccgct gctggtgccc atcgcccagc
     3541 acttcggctg ggacctggtg tggttcggcg tcatcatcac gatgaacatg gctctgggtc
     3601 agttccaccc gcccctgggc gtgaacctga tggtcacctg ccgcatggcc ggcgtgtcca
     3661 tggagtccac ggtgccctgg gtgctgtgga tgatcgcggc gatggcgggc accatgttcc
     3721 tggtcacctt catccccgac ctggccctgt ggctgccgcg ccacctgggc tatctgtgag
     3781 ccgaccgcgg gggcgtgggg cccccaccgg gtgattctca gtaggtcatc agcagggaca
     3841 cgttgcgggt gtagtcggtg tccgacgccc gcagcggcgt gaaaccgttt gacgtgtcga
     3901 tgcggtactg ggtgatcacc acctcgacct gcgcgctgga cgtgtcgtag taggtctggg
     3961 tcagggacag gatggccgtg gtggcggtgt cggcggccag tccgtaggtg gtccgcaccg
     4021 tgccctgcag gctggtcttg gagctatcgg cgtaacgatt ggcggtggcg acgaggcccg
     4081 cgtcgcccac gtgggcggcg ctgggcaggg tcggtgtgcc ctgcaccacc acgtactcgc
     4141 cgccgacggc gccgacgggc acgtagtggg tgtcgctcca gtccaccgtc gaggtggtga
     4201 ggggggtggt gacaccattg accgtcacgg acccggtcac cgtggtggtg cgctgcaggg
     4261 ccgggaggcc ttcaaaggtg ccggccgtga cgctgccggc gacctcggtg ccgctgcccg
     4321 tgacggcgta gccgctggcg gtgccgctga gggtgaatgg gcgggcggct gcggtagtga
     4381 actcggcggc ctgcacggcc tgcaggttga agctggcggt ggaagcgacc ggtacggacg
     4441 tgcctccgcc gccgccgccg caggcgagca gcatcaggga gaggggcagc gcgacgagga
     4501 tggcacgcgg gggggagaaa cggcgggcag acatggcagg ctccggacag acgggaatat
     4561 cagcgagagc ggatattacg cccgacaggg ccggacaggg gccgtggatg acgatgttct
     4621 tgcgggcccg tgtcggctgc cgggcgctcc ggatgtggct ttcaggcgct gcgtacgcgg
     4681 cgcgacgcgc cggtgggttg aagcggcgcg gcgctggccg gacgcgcggg caggcggaag
     4741 cggccgacgg cggccaccag attcgccgcc tgctcatcca gactctgtgc ggcagcggca
     4801 gcttcctcga cgagcgcggc gttctgctgg gtgacttcgt ccatgcggcc gacggcggtg
     4861 ctgacctgct cgatgccgac actctgctcg cggctggcat tgctgatgtc ggtgaccagc
     4921 gtggtgacct ggccgaagac ctcgaccact tcttccatga catgcccggc ctcgtcggcc
     4981 aggaccgagc cgtcttccac cttgcccacc gagacggcaa tcagttcctt gatctcgcgg
     5041 gccgagaccg cgctgcgctg ggcaagctgg cggacctcgt tggcgaccac ggcaaagccg
     5101 cgaccctgtt cccctgcgcg ggccgcctcg acggctgcgt tcagggccag gatgttggtc
     5161 tggaaggcga tgctgtcgat gacgccgatg atgtcggcga tctttcccga gctttcgcgg
     5221 atgtccgcca tggtggcgac cacccgcttg acagtgctgc cgccctggtc gaccacgtga
     5281 caggactggc gcgtgaggtc atgggcacgt ctcgcactgt ccgcgttctg ccgcacggtg
     5341 gcgctgagtt gctccattga actggccgtc tcttccagac tgcccgcctg ctgctcggtg
     5401 cgggcagaca ggtccgcgtt gccggcggcg atctcgcccg cggcggtggc gatggcctgg
     5461 gcgctctgct gcaggcccgc caccagcttc tgcaactggg tgacggtggc gttggcatcg
     5521 tcccgcagcc gcgcgaaggt gccctggtac tggccgtcga tgcgctgggt caggtcgccc
     5581 tgggcgagtg cgcccagcag gcgcgagacg tcgcccagtc cggtgtcggc cacatccatc
     5641 agtcggttga ggctgaggat catggcgcgg aaggcgtggt caaaggcttc ggcgtcgccg
     5701 cgggcggtga aatcgccgct cgcggcggca tccaccaggg tctggatctg cgcgtttacc
     5761 cgctgcaggt tgcccttgac gctgtccatg gccgcggtga tctgggcctt ctgaccgggc
     5821 aggcgcgcca tgtccaccga gaggtcgccc accgcgtagc gtttgaccac ctccaccacc
     5881 tgcatcttca cggcgatatg ggcctgcacc agagcgttga tggagtgcgc catgtcgccg
     5941 tagacgcccg ggaagcgggc cgcgtcgatc tgctggtcga tcatgcccag atcgtgctgg
     6001 tgggcgttct cccgctgggc gtccacaaag ccgcgaaggg tgccaaccat ctgctgcagg
     6061 gcttccagga gctggcctgt ctcgtcccgc gtcgcaatgt cgatgcgctc gggcagggtg
     6121 ccggcggcga ctgcctgact gatggcgaca gcctggctca gggggcggac gacgctgcgg
     6181 ctgatctgcc aggcggccag cgcccccgcg acgaccatcc cggcggcgag cagtccaatc
     6241 aaccgggcgg tgcggccgac ggccgcgtcg aaggcctcgc gggctgcgcg ggtatctgcc
     6301 gactgggtgt cgatcagggt cgccaatgcg gcgccaatcg gtttgtcggc cccggcgatg
     6361 gccttgtcca cgtcgccgat ggacgccccg ccggcgcgca gctccatggc ccgcgccaca
     6421 gcgctgcggt aacggacgct gccggcccgg atctcgccga gcgccgcctc ggcctcggcg
     6481 ccgaccgggc caagggcgcg gtactcgtcg gcgatgcgtt ccagcgcgtc catgctctgg
     6541 gcgaatttgg ccgggtagtc gccgccccgc aagaccgtgt tcttgaaata gtggatgcca
     6601 ttgccgagca tggcctgccc gcgcagggcc aggcgttccc gcgccaccat ctgtgcttcg
     6661 tacgcaccga actgctgctg cagcgcgtac aggttgtaga tggcgttgac cgccacggcg
     6721 aggccgaggg cgatcacgaa gccgaagccg agggccaggc gacggcggat ggtgagcggc
     6781 ttcatggcgt gattctccgt gtcgggctga cggctggggc aaggggggcg acgtcgatgc
     6841 gatgaccggt gctgcggtca gccagagggt gcaggggcat ggtcggtctg ggggcgggtg
     6901 acgggcgtcc aaggggcgag gggcgtggag cgaataacga ccgtcttgag gcaaacatga
     6961 atccggtcgt cgcggcgggc aggcgtgtga cggtccgatg gccgcagatc ctgatgcctg
     7021 cgtggtcctg gcgcaggtcc gggcaagtcg ccgggcatgc tccgatgccg catgcgtggg
     7081 gtggtcttgt gcgcgcctgc ttcgggcatt caaggtcgcc ggatggccgc cgataggagg
     7141 cgtaacgcgc cgaatgggcc cacgatgaga acaagaggag acaagacatg aagctgttcg
     7201 acaggtttct gggcgggccg gtggccgagg ttcagacgct ccagtgccgc agtgccgaac
     7261 tgtcggcacg cctcgccgcg gtgacggcgc cgccggcctt cgtctgtggc ttcgtgtcgc
     7321 cccatgtgga cctggaaccg gtggcacggg cggtgaccgc ccggtttccc ggcgtaccgg
     7381 tgatgctgtg ctccacggcg ggcgaactgt gcgccacggg gcgtgacctg tattgcccga
     7441 ccggcgatgc ctgggaccgg gtggtggtgc agctcttcga cgcgaccctg atcgcccgtg
     7501 ccgaagtggt ggccgtgccg ctcaggtgtg aagacctgcg gggcggtcgg tgcgatcagg
     7561 acgtccgtga gcgggtggcc accctgcgcg aggagatccg cagcctgaag gtcgagatgg
     7621 atatcgacca tcgggacacg ctggcctata tcctgttcga cggactgtcc gcgtccgagt
     7681 ccttcttcat ggaggccctc tacgattccg ggcgtttccc ctgtctgttc gtgggagggt
     7741 cggcgggcgg caagctggac ttccgccaca cctggctgca cgacggccat cgcaagctgg
     7801 agaaccatgc cctcatcgcc ttcctcaagc tgccccgggg gatgcgcttc ggggtgttca
     7861 agagccacaa cttcgaggag gcgggggcgc acttcccggt cattggcgcg tcgctggagc
     7921 agcgcttcgt gcgccaggtg atcgacaagg aggggcgcat cgtgtccttc atcgacgccc
     7981 tgtgcgagga gctgcgctgt acgcctcacg aactggacgc gcgcctcgcg gactattcct
     8041 tcgccatccg ggtgggcgac gaactgttcg tgcgctccgt ctcgggcatc gacctggccc
     8101 acgggcgggt gggcttctac tgtgacatcg cggcgggtga agacctgctg ctggtgcgtc
     8161 ggaccagcct ggtgcacagc accgagcggg acttccggac cttcatggag ggcaagccag
     8221 gccagccggt ggccggcatc ctcaacgact gcatcctgcg gcgcctctac aacgggccgg
     8281 agctgggcaa gctcggatcg gtgctggcca gtccgtcgct ggcaggtttc tccaccttcg
     8341 gcgaaatcct gggcctcaac ctcaaccaga ccctgacggc gatcttcttt ttccgggcgg
     8401 cggagggcgc cggcttccgt gacgagttcg tggacaactt cgtggcccat tacggggagt
     8461 tcaaggcctt cttcctgcgc cgccagagca acaagctcac cggcctctcg cgcgtcatcg
     8521 tccagcagat cctgtcctac caggccgggc agttcgacag ccagctcgac ccgtcgggct
     8581 tcgacgacaa catctcgctg gtggcccatg ggctcaacac cttaggtgag accctcaagg
     8641 cgtcccatgt cctgcgggac gacacggcgc gccagctcga ggcctgcgcg ggcgacctct
     8701 acgcatcgat ggatgccctc ggaggtgacg tgcacgaaca ggagcgggtg gtgcacgatg
     8761 ccggcgggcg ggtgtccgac ctcagccgcc aggctgccga tgcggccggc agcgcccgcg
     8821 acctggcccg cgccagcaac cgcatccaga gcgtggtgga ggtgatccag cagatcgccg
     8881 accagaccaa tctgctggcc ctcaacgcag ccatcgaggc cgcgcgggca ggcgaggccg
     8941 gccgcgggtt tgccgtcgtg gccgacgaag tgcgcaagct ggcggagaag tcccgcacca
     9001 gcgccggcga gatcggcgcc gacatctccg cccttgccgg cgagatcggt cgcgtcgccc
     9061 tgcagatcga gcagcaggcc ggcgatgtca cgcgcctgtc gtccctgctc acggatatcg
     9121 cggggatcag cagtcgcacc gcgggcaacg cggaccacac ccgcaatgtg gccgaccgcc
     9181 tccggcagct cacggaagcc cagctgaacc agcattgatc cggcgcgatt gcgccggctc
     9241 gctgcggctc atgcagggct gcggggcgga gactttcagc gcgcccgtcg cagccaggcg
     9301 ctgacgctgt ccacggcgat cgccatgacc agcatggcca ggatcaccgt gccggcttcg
     9361 gcctcgcgga acagggacag gctgtagtac agcaggccgc ccagcccgcc ggcgcctacg
     9421 aaaccgagga tggcagccat gcggatgttc atctcccagc ggtacagggc gtaggcgagc
     9481 cattggtcgc ccacctgcgg cagggtgccg tagaggaagc tggcgagggc gggcgcgccg
     9541 ccgccggtga gggcttcggc gggcgcggcc ggcgcgtttt cgagggcctc ggcaaagagt
     9601 cggccgagca ctccggtggt gtgcacggcc agcgccaggg tgccggcgaa ggggccgagc
     9661 ccggctgcca ggaccagcag ggtcgcccac accagttcgg gcaccgagcg cagcagattg
     9721 agcacgaagc gcgccacgat ccgcgccgga cggccatggc ggcccgaggc ggggagggcc
     9781 agcagggcgc cgcccgccgc tgccagcagg gtggcgacga gggaaatggc cagggtttcc
     9841 agcgctgcgc cgccgacctt ggccacgaag ccggcgtccg tctccggtgg caggaagcgt
     9901 cccgcgaagt ccagcgcctc tgttgccgcc gcgccggtga acacggccgt caggggcagg
     9961 tcgaggaaga cgaagctccc cgccaccgcg gccagcaccg ctagaccggc gagcaggccg
    10021 ccgggtgtga cgccggccac gcgtggggaa gaaggggtgc gtacgttcat gccagccggc
    10081 tccgcagcca ctccgagagg gcgtcggcag ccaccacgag gagcaggaag ctggcgagga
    10141 tggtggccgc ctcgccgccg ttgagcatct tcatggactg gtccatcaac tggccgagtc
    10201 cgccggcgcc gacgaagccc atcaccaccg aggcacgtac cgcgcattcc cagcgataca
    10261 cggtgtagga gaccagctcc ggggcggcga ggggcagcaa gccgtagctg aaggccgcca
    10321 ggcgtccgct gccggcctcc agcaggccgc gggccgggcg ggcgtctgtg gattcgagga
    10381 tctcgccgta gaccttgccg agcatgccgc cgtaggtgat gcccagggcc agcacgccgg
    10441 ccgccggacc gagcccgaag acccgtacga acagcagcgc ccagaccagt tccggaatgc
    10501 ctcgcagaag ggtcagcagg ccacggacga gggcgcgcag gagggcgccg gtgcggctgc
    10561 cccgcgcgtc ggccagacgc gacacggaca ggctgcgggc catggcgagg cccagcggta
    10621 aggcgagcag aaaggccacg gccatgccgg cggtggccat cgcaagggtt tccacggtgg
    10681 cgcgggcgag gagggcgagg aagtccggat cgcgcgccgg cggcaggaag ccgccgagaa
    10741 actggcccac cgtgcgcagg ttgccggctt cgaacagggc ggcggggcgg aagtccgtca
    10801 ggtgcagcag tggccccagc agcgccaggg ccagaacggt ggcgcccaga cgggggaggg
    10861 cggccgggtc gcgcggggtg gggctcatgg ggttgaggcg agacgggcgg tgcgcgggtg
    10921 gagcgccggt gggccggcgc gcgtgggttg gcgtccgggc tgatcggggc acggcacgta
    10981 gcgtgggtcc ttgccggggc gattcacgag cagcgcctcg aaggctgccc ggccggacaa
    11041 atgctggcga cgggcagccc gccttcggcc gcgtacaggg tgcgcagctg ggcggcgctc
    11101 acctcgcggc tgggcaggtc aaagaggatg cggccgtcct gcaggccgac cacccgcggg
    11161 aagtggcgca gggccagatc caccgcgtgc aggctggcca ccagggtgac gccgcggcgg
    11221 gtcgcttctt cgttgagcag ggcgacggtg agttccgaca gggccggatc gagggcggag
    11281 accggctcgt cggcgaggaa gaggtcgggt tgctggtaca gcacccgcgc cagggcgacg
    11341 cgctggagct ggccgccgga aaggcggtcg cagcgttcgt agagtttgtc ggtcagatcg
    11401 aggcgggcga gctcgcgggc ggcgccgggg gcatcggcgg gcaggacgag ggaggcgagg
    11461 gccttccaca ggggccagtg gccgagacgg ccggccagca cggcggtcac cacgcgctgg
    11521 cgcggcggga tggggggcga ctggtgcacc agcccgatgc gtgcccgcag gcggcgcagg
    11581 gcggccggcg aggcgctcca ggggtcggcg cccagcaggt gcaggcaccc ttccgtcggg
    11641 cgccgggcgg cggccagcag gcgcagcagg gtggtcttgc cggcgccgga tgcgccgacg
    11701 agggccaccc gctcgccgtc ggcgatggac aggtcgatgc cctgcagcgc acggtggccg
    11761 ttggcgtgga ccaggcccac gcctttgagg gagaagccca tggcggtctt tcggcggtcg
    11821 ggcggcggcg cgctgccggc gtccggccgg ggcttacttg agcaggcccg cggcgtgggc
    11881 cgcggtttcg atgccggcgt agttttcggc cttggtgggg atgaacttgc tggcgcgctg
    11941 cagggccatg atctccttgt gggcggggtt ggccgggtcg agcttgagga aggcctgggt
    12001 gatgcgggcc tggagcttgg gatccaggtc gccgcgcacc gtccagttgt agtcgaagaa
    12061 gggtggcgtg gtggcgaaga cgcgcacctt ggcggtgtcc accttcttct gttcaagcag
    12121 tttgtcccac acggaggcgt tgagtacgcc cgcgtccacc ttgcctgcct ggacgaaggc
    12181 cacggtggcg tcgtgggcgc cggagaaggc gacggtcttg aaatccttgt ccgggttgag
    12241 gccctcctgg ccgagaaaat agcgcggcat caggtggccg gaggtggagg acggcgcgcc
    12301 gaaggcgaag gtcttgccct tcaggtcggc caggctcttg atgccgtcct gggcggtgat
    12361 gaacttggac gtgaatttgg cgtcctcggc ccgctgcacc aggggcacgg cggtgccgtg
    12421 ggtgcgcagt ttggcctgta cgaaggtgaa accgcccagc cacgccatgt cgatcttgcg
    12481 ggtggccagg gcctcgacca cggcggcgta gtcggtgacg ggcacgaagc tcaccttcat
    12541 gccggtttcc tgttccaggt aggcgccgag gggggcgaac ttgcgttgca gttcggtggg
    12601 ggcttcgtcg ggaatggccg agacgcgcag gacgtcctcg gcgaaggcgg cgcagggcag
    12661 gccggctgcg gcggccagca gcgtcgccgc agcggcgcgc agcagcacgc gccgtgggaa
    12721 gggcacggaa atcatggttc gctcctcaag ggtgtgcgtc gccgcagcac ccgccgccgg
    12781 gacggggagg tcggccccgg catgatgggt ccggtgaacg ccccaggggc gcgcgccggg
    12841 agtcaggctt gcatgcggta cacagaccgc ggggcccgac tatagcgatg cggcggggcg
    12901 acgggaagag cggagcaagg gggagcggag ccgcggggcg tgccggcagg cctccgccgc
    12961 gggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaccgag ccgccgaaga
    13081 cttccttgaa gtaggcttcg tactcgccgg cttcgtgggc ggcgatgctc aggctgtggc
    13141 gttccacttc gacgcggtga tccagttcgg ccgtcaggtg gtcgtaaagg gaatcgagtt
    13201 tcatgatggt cactcctgaa aagttgaaac aggggagcgc ccgatccggt acgcccggtt
    13261 ctgcgaccgg gttccggact ctccgtcggg gtttccatga cttcactata ggggcgatgt
    13321 tgcgctgcaa caaacgactc tttatgatgg aagtgcttag tttttctaag gtgtgcagtc
    13381 atggcctacc gtctgcctcc cctctcgtcc ctgagggcgt tcgaagccgc cgcgcgacat
    13441 ctgagttttg ccctggcggc acgggagctg cacgtcacgc cggcagcggt gagccatcag
    13501 atcaaggccc tggagtccta cctcggcgtg gccctgttcg agcgcctgcc ccgcgcggtg
    13561 gtgctcacgg cccagggacg cgcgctgctg ccgcgggtgc aggagggctt tgaatgtttc
    13621 gccgcggcca ttgaggcggc gcgggaggcc ggcgaagggg ggctcacggt gcacgcgcca
    13681 ccctctttcg cccagcgctg gctggtgccg cggctggcag gctttgctgc ggcccatccg
    13741 gaaatcgccc tgcgtgtggc gagcgatggg gccaatatcg acggtgtcgg cgcgccgccc
    13801 gaagaccgcc tcgccgatct ggcggcagag ccggacgtgc tggcgatccg cttcggcaac
    13861 ggccgctacc ccggcttccg ggtggagcgc gtcctgacgc cggactacat cgtggtgggg
    13921 cgccccgatc tggtggccgc ttcggccctg cgtcagcccg ccgatctcct ggcccatccg
    13981 ctgatccacg acgagtccat cccggacgcc agtgtgcgcc cgagctgggc cgagtggttc
    14041 cgccgggtcg gcgtggcaga cgccgacgtg agccgcgggc cgcgcttttc cagtgccgtg
    14101 ctggcggtgg aggcggccct cgccgggcag ggcgtggtgc tgaccctggc gcccctggtg
    14161 gcggccgaca tcgctgccgg gcgcctgcgc cagccctttc ccgagcgcct cgcctcgccc
    14221 tacgcctatt acatggtgac cgccgaagcg caggccgagc gccgtcccgt gcgggccttc
    14281 cgggactggt tgcgggccga gctggcgcgg acgtccggtg ccgtggaggg ggctggcagc
    14341 gctgaaaagc gcgggtgata taatcgcccg ctggaaaatc ctgcccgtcg ccgggcgttg
    14401 cgcatgcaac tgtttgctct cggactgaac caccataccg cgcctctcgc catccgcgag
    14461 aaggtcgcgt ttcgtccgga gcgcctggtg gaagccctcg gcgatttcgt ccagcgcaag
    14521 ccggtgaagg aagcggcgat cctgtccacc tgcaaccgca ccgagatgta cttcgccact
    14581 ggcgaacccc agtccgccgc cgactggttc gccgactacc accgggtggc gctgcaggaa
    14641 ctctccccct acctctacac ctttccccag cgtgacgcgg tgcgccacgt cttccgcgtg
    14701 gccagcgggc tggattccat ggtgctgggc gagccccaga tcctcggcca gatgaaggac
    14761 gccgtgcgcc aggccgaaga ggccggcacc ctcggcgcca ccatgcacaa gctcttccag
    14821 cgcaccttct cggtggccaa ggaagtgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnng acgacccgcg caaggtcctc gaagccctca gcctgggcct caccaacaag
    15001 tttctccacg cgcccacgcg ctttctcaac gaagccgacg gcgaccagct gggcgacagc
    15061 gccgagctgg tgcagcgcct cttcaacctc cacaaggacc gctagccccg cccggcagcg
    15121 cccgtctgcc cgcgcccgcg ggccgtctcg cccatgaaac agcgcatccg cgacaagctg
    15181 gacaccctcg ccgaccgcct ccaggaactc gaccgcctgc tcgccacgcc cgaagcggcc
    15241 ggcgacatgg agagctaccg caagctcacc cgcgagcacg ccgagatcgg ccccgtggtg
    15301 gccctctacg gccagtacac ccgcgccgtg gcggacgagg cggccgcgcg ggagcttctt
    15361 gccgacccgg agatgaaaga cctggccgcc gccgagctgg acgacgcccg cgccgcccag
    15421 gcccgcatcg aggccgcgct gcagaccgcg atgctgcccc gcgaccccaa cgacgagcgc
    15481 aacctcttcc tggaaattcg cgccggcacc ggtggcgacg aggccgccct gttcgccggc
    15541 gatctgttgc gcatgtacac ccgctatgcc gaacgccagg gctggcgcgt ggagacgatt
    15601 tccgccagcg actccgagtt gggcggcttc aaggaggtga tcgtgcgcgt cagcggcgcc
    15661 ggcgcctatt ccaggctcaa gttcgaatcc ggtggccacc gcgtccagcg cgtgcccgcc
    15721 accgagaccc agggccgcat ccacacctcc gcgtgcaccg tggcggtgat gcccgaggcc
    15781 gacgaggtgg gcgacgtgga gctcaacccg gccgacctgc gcatcgacac cttccgtgcc
    15841 tccggtgccg gcggccagca tatcaacaag accgactcgg cggtgcgcat cacccacctg
    15901 cccaccggca tcgtcgccga atgccaggac ggccgctccc agcaccagaa caaggcttcg
    15961 gccctgcgcg tgctggcggc gcgcatcaag gacgtgcagg tgcgtgagca gcaggcccgc
    16021 gaggccgcca cgcgcaagag cctggtgggc agcggcgacc gctccga
//
LOCUS       DAOQMM010000088        15806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1446_length_15806_cov_46.375087, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000088 DAOQMM010000000
VERSION     DAOQMM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15806
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1446_length_15806_cov_46.375087"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PLH85_13125"
     CDS             complement(<1..1334)
                     /locus_tag="PLH85_13125"
                     /inference="COORDINATES: protein motif:HMM:NF023282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HNB65554.1"
                     /translation="MSTQQQFRQTALVIALSAVFPLSSAWAEDDDVAQLISPNVTEVT
                     VNVPWVNEVNPLYRQYNGINNQGVNANLDLNIVRRSDDAEWFRLNARNLGLSTQEFGV
                     SMEKQGDWALGLNYDQIPRYAPYAISTAVSGIGTNTITQPSYANSAFTSGSLTNPNLY
                     DVTLKTERDIVTLNASKFVAKDVKLSFMFKNEEKSGLRMDGVRGVAGTNGSTGGTANR
                     YSGFLFSPEPIGQTHKQFEATLDFTNEKYQITAGFYGSFLNTRDNSLNVIPGTNTQLV
                     TTTAPLLSPIALAPDNSMQQFFVSGAYNFSPDTRANLKVSYSDARQNDGFFSGQPTMT
                     GIGTSLNGEVRTTEVFSSVTSRINKNLKLLASWRYEDKVDKTPIRQYVPTSFGEPYNN
                     PESHTANWGKLEADYRLGGGYSVTAGVDYTNKASLEWERNKVSETTTRLAVR"
     gene            complement(1350..2336)
                     /locus_tag="PLH85_13130"
     CDS             complement(1350..2336)
                     /locus_tag="PLH85_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HNB65555.1"
                     /translation="MKIFRKLFASCAVFGALVAAQASLAADAPATPAAKPAAKDLVLK
                     GDAKCTSCHDETDEPKLLHIGKTKHGSVADGRTPSCTNCHGESDLHTNNPAKLKERPP
                     TDRNFGKLSKLSAEDKSAACTTCHQGGKHINWNTSAHANRDVSCTSCHQIHSPKDKVR
                     DKRQQAEVCYTCHKQQRAEANKPSHHPIPEGKMTCSDCHNAHGSAGQKMLVKDTTNAT
                     CFTCHAEKRGPFVHNHQPVVEDCGNCHNPHGTTAEGMLKSRQPFLCQQCHGDSSHPGN
                     VPGVRSLMPNAVSTNIGPGMAQARGCMNCHTNIHGSNSPSNATSSGPTRFFR"
     gene            complement(2408..2752)
                     /locus_tag="PLH85_13135"
     CDS             complement(2408..2752)
                     /locus_tag="PLH85_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB65556.1"
                     /translation="MKIRTVCLATAALATALSAGSALALDADAAQALAKKEGCTKCHA
                     VDKKKEAKSLTEISKSLKGKADAEAKLLHHLTSGEMVKFEDGKEEEHKILKTKDKAEI
                     KNLTDWILSLGK"
     gene            complement(2799..3413)
                     /locus_tag="PLH85_13140"
     CDS             complement(2799..3413)
                     /locus_tag="PLH85_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB65557.1"
                     /translation="MTKRVYMLAPWIRLWHWGNALAIITLSITGISLHFSGSELALVE
                     FSMAARIHNVAGVCLAVLYSVFVIGNIVTGNWWQYVPKPPGVLGRCLKQMQFYGSGIF
                     KGEEEPFPPTPEVNFNALQAVTYWSIMYLVVPTVIVTGLIFLYPQFAPDQIFGMDGLL
                     PIALVHYLAAAVILLFMVSHIYLGTMGPKVSSLFKMMITGWYEH"
     gene            complement(3406..4377)
                     /locus_tag="PLH85_13145"
     CDS             complement(3406..4377)
                     /locus_tag="PLH85_13145"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB65558.1"
                     /translation="MFKKFKNRVRGALGLLLLGFAALAMADDAPPTPEELAKIRTANA
                     ECFKCHSEAGLRTPVKEGMDLAKLRTLLVNPDTYAASDHGEMACSKCHGDGFDEHPHA
                     PKAKEDLAECQDCHARKAMRIERQFDKSVHAENLSDRFSCATCHNAHTMSFAAKLRDP
                     HKIVAQDNKICLDCHDNDEAFAKMAPDKKKRPAIDDIHKWLPNTRLHWQAVRCIECHT
                     PTDDKLSLSHEVQNKDKAEKKCVTCHNPDSSLKVRLYRHLAKEEQQKYGFLNSVILSN
                     SYVVGATRNPTLDLLLIGAFVATVLGVLGHGLLRIITTRIRRSKKND"
     gene            complement(4573..5373)
                     /locus_tag="PLH85_13150"
     CDS             complement(4573..5373)
                     /locus_tag="PLH85_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNB65559.1"
                     /translation="MTQPSPILSALYRYPVKSMAGDALNASAVSRLGLPFDRMWMVAD
                     RSGRFVTGRQLPQLVRVRTEPSADGVTLTMAGREALFVPNEVFTEEVSAVVWGSLFPA
                     WGGAAAADAWLSDALGAPLRFLWTGAQTARRVRVEPDVPLSFADGFPLLLIGQSSLDD
                     LSARVGRPLEMARFRPNLVVSGAEAFAEDGWTRIRIGTVSFRLVKPCERCVFTTVDPV
                     TGEKSLDQEPLRTLARYRKTADGVLFGQNVIADGAGELVCGMPVDVLE"
     gene            5567..6406
                     /locus_tag="PLH85_13155"
     CDS             5567..6406
                     /locus_tag="PLH85_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNB65560.1"
                     /translation="MPALLRALFLLSLALCARVASADGMRDLEIGVLPNLSTRVLMTQ
                     YAPLRDALARSLNRPARIATAPDWSRFTRRGLEGDYDLIVTAAHLARLQQVEAGLIPV
                     GRFTPDIPALLVTRGDAPVSRLTDLRGKIVALPNVHSLVALQSLHRLAEAGLRPGQDL
                     RLISVPTDDSVGNLLLRGDATAAVLSGGEFRMIPEDIRARLKVVQTVAEVPGFIVLAS
                     PRLDADALERLRRCLESFPGSSDGRRFAELTGFSGLTTVDDALMKSLDIHLPDARQIL
                     QAP"
     gene            6403..8049
                     /locus_tag="PLH85_13160"
     CDS             6403..8049
                     /locus_tag="PLH85_13160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB65561.1"
                     /translation="MNALWPASLRFRLLVASLVVGSISLALLIAHSARLIEASLLGQL
                     NRQLEQTRPLLNAALNAPLAERDYATLRQILHEARTPEMLTYLVLLDHRGEVIASEGR
                     PASTPLPAIDPPAQFDLDRPDRVFHTEVHIDLAGQALGTLRFGLSTRFLHDERQRLIR
                     ESLELGAAVLLVALFALGWVSLWLTRRLARLTDASRQLAVGHYDVELESSGSDEVDQL
                     STAFLRMAATIKVRVEELVLSEALQRRYYEHARDERARLASLLSALKTGILFVGNDGI
                     VVYYNATFGQIWRIPPEAGLVHQPALRLRPYVERLLDDTGSFMAIPLADADLAEFRLL
                     DHRIVTQTTSPVLDETGTSVGRIWIYEDITHQRQTAELADQAERDALTGLYNRRGFDL
                     ALDRMARTARERNANLALCFIDLDGFKRINDCYGHRAGDAILVQVASEFQAQVRRREC
                     LARVGGDEFAVLVPDVSEEEITLLAERLVKAIAGMPIRYTGQDLRLGCSVGIAIYPWH
                     TRQPDDLMACADAAMYLAKDAGKNRWCMLPTAPPRHAGDT"
     gene            8102..8434
                     /locus_tag="PLH85_13165"
     CDS             8102..8434
                     /locus_tag="PLH85_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65562.1"
                     /translation="MPPTRAALAATALIVLMATACADAPTRTATGVRILVEFTTPTDG
                     AAPATRAFLEATGGVPVRYVTAVSGRTHAYRLACPPQDDTCSAALDALRRAPAIRSVT
                     PDHLKDTP"
     gene            8431..10290
                     /locus_tag="PLH85_13170"
     CDS             8431..10290
                     /locus_tag="PLH85_13170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNB65563.1"
                     /translation="MTSTLRGTCLALLALTGLSLLPGAARADDAVGGRVIVKFRSSSA
                     LKQIAAAGRVQSLAQRLGVTATLIGQPAPELHVMHARGISTAALAARLAQESDVEFAV
                     PDRLKQAAALPNDPLFSAQWMLQTPDTTHPAAIDAVGAWTLSAGSSGVVVAVVDTGVR
                     LDHEDLMSRLLPGYDFISDDLNAGDGQPGRDADPADPGDFVSSADLANSAFTQLCPGV
                     SRASSSWHGTQIAGIVAAEANNSLGIAGVAGATRVLPVRALGKCGGFDSDIIAGMRWA
                     AGLSVPGVPANTHPARVINLSLAGSGSCSAAYASAIAEIRAAGTLIVAAAGNETGPVE
                     EPANCAGVLAVAGVRHVGTKVGYSSFGAEVGLSAPAGNCVNTATGSPCLFPFVNATNL
                     GSTVPAANGYTGLTAQTVGTSFATPLVAGTAALMIAAHPGLGVTALGERLRASATAFP
                     SDASLAACPTTTADGQCNCTTATCGAGMLNAKAAVQAAQAPEAAVAVSATPVAGSAVT
                     LSAAASTTAPGATLVAWQWTQTAGPASAAFSNAAAASTSFNAPQAGTYSLRLTVTDDA
                     GRQGSVDTTLDVQAPPPPAEGGGGGGAVDGPLLALLGAAASLAIAARRGVRRP"
     gene            10323..10670
                     /locus_tag="PLH85_13175"
     CDS             10323..10670
                     /locus_tag="PLH85_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNB65564.1"
                     /translation="MRYTGSCHCGHVAFEAEGEITGLLECNCSICRRKGALMWFVPRS
                     AFRLLTPEENAATYTFASGRIHHRFCPRCGIQPYGEGDDPTHGPMVAINARCLDNVDL
                     AALPVRHFDGASL"
     gene            complement(10730..12091)
                     /locus_tag="PLH85_13180"
     CDS             complement(10730..12091)
                     /locus_tag="PLH85_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65565.1"
                     /translation="MVDEAGTPGCAGPDGATQRIGEAAEKGARGDVRGADGVNGARAG
                     DVELARWLDPIHRLSELLHAAACGLAGVCHPRTQVALAVRVMRVQYLLRERTGDLEAA
                     LALFEGGGRRFMSAVDAALKDAAAGYPEDGLLRLARCTPHLDCMRQGVQGLCGELDTV
                     REAVDAALAAVAEVYAPQDADWSREAHTRGRVFECRADVAPGWADDESGAGAARLTSE
                     RRPIRCDAEEASAPVPVPQAARPGPAVGAGEGPAPPHDGPSGALPAVCEADAEGGDGG
                     AWAFAEAAHEAALNADLNALAMAADGAAVEAAAARLLCHARVELARMAGMLTRLGGHG
                     QVLIEGGRRLAGETVPGWIRGNGPHGTGDTDSRAGDERARAEARVRAGWHAQMQAVQA
                     LRGGLTGLLREMGLRYAFNPLPEAGRREAVAWARAWLGAADAIRCGEAAPGGEAIPPA
                     RLI"
     gene            complement(12148..13110)
                     /locus_tag="PLH85_13185"
     CDS             complement(12148..13110)
                     /locus_tag="PLH85_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65566.1"
                     /translation="MKRSLHGLVGLWLLLWGLAWADGAQLQRVTRVFDALHQSEAALA
                     AGNTSRAQALLTDIRSAADGLRQDTETYVNRARGAADRRQADVLRTVQEIDETFRLEG
                     EADREMRDRQARIADLDVQLAEVARVRVALDARMAELRQEGRTRDECRAQPLEGMFYS
                     WECWRLSFADVFANRWKALNNEAADNQRQRIQIEMDRQGAGNERDRAQRSLDMLRARK
                     ADLQARRSQLDREQNTLRAAVTRLTDAVQFWRDLGVLVQSNIGTVAMLQDSVQRLAQR
                     ASTQAPTPVFDRYDAETVRSLEDTLKDFARSIDDGRNILLADGA"
     gene            complement(13209..15155)
                     /locus_tag="PLH85_13190"
     CDS             complement(13209..15155)
                     /locus_tag="PLH85_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65567.1"
                     /translation="MSDPVPATPPVVSPPSPVIVFDTMTYNVIEGPDDSSPLVQAAHA
                     QILQTLEIDKLVRNLNRTDDLLHIAACGVAGFLNPESGKSLSAQVMNLQYKLRDKTGE
                     MSSALLSFGEAAGRMIPILRGAFKDLYGFYEADCVGRLTGCEAVATSMATTAENLKST
                     FRGLADEAQKIAEDTTQTRDLRRKAKEAAQARQKEIKADLEEKERLKASLAEQIPQVK
                     GWYEEAKAAQNTADGRMFALAIVGSITKALGDGLAAGLQFKTAPVTAGTQLATAIVKN
                     TAVKEPDEKNKKTDDKAKATSETLTAQAAYRVAKDALDQAEAKLTAAQTATKTAQGKL
                     DKLEEPYIEARDAYEAARKKKPKDKKDEAAKKEAFEELEEPYKEAEDAVEDAEAAEEA
                     AQKAVDAAKVKESTAKAVMEDAAAKAPLAGVAEGLRSAGASAQEMGASYADIAANYAK
                     EKAKYLDMLMDLQKQEREALGAIAKFAVQMQTEKDTEQLEQAAVESLHIAVGVLKYVV
                     VILQDVCQFWTQMAAACRRLASADLRTDIEVYMRRTKEERVKEYSAEDFKLRMLAVAA
                     QWRALKRVATEYRAATMQVFDKLGVTYRDNLPIEQARAEAKRLAQLLDKDVKDEIKKT
                     DDATQQIAAAKAEADKLIAKAAAA"
     gene            complement(15275..15508)
                     /locus_tag="PLH85_13195"
     CDS             complement(15275..15508)
                     /locus_tag="PLH85_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65568.1"
                     /translation="MGDKFYPDKGEPHIHEHGGGIDFTDTRHHHTKLLHGNELREPAC
                     IKVIDDLKEDPTEREQQIIDYIKALVRKYKKGR"
BASE COUNT         2285 a         5838 c         4997 g         2686 t
ORIGIN      
        1 ttgcgcacgg ccagccgcgt ggtggtctcg gacaccttgt tgcgctccca ttccaggctc
       61 gccttgttgg tgtagtcgac cccggcggtc acgctgtagc cgccgcccag acggtagtcg
      121 gcttccagct tgccccagtt ggcggtgtgg gattccggat tgttataggg ctcgccaaag
      181 gaggtgggca cgtactgacg gatcggcgtc ttgtccacct tgtcctcata gcgccaggag
      241 gccagcagct tgaggttctt gttgatgcgt gaggtgaccg acgagaacac ttcggtggtg
      301 cgcacctctc cgttcagact cgtgccgatg ccggtcatgg tcggctggcc ggagaagaag
      361 ccgtcgttct ggcgcgcgtc ggaatacgac accttcaggt tcgcccgcgt atccggcgag
      421 aagttgtagg cgccgctcac gaagaactgc tgcatggagt tgtccggcgc cagggcgatg
      481 ggcgacagca gcggcgccgt ggtggtgacc agctgggtgt tggttcccgg gatcacgttc
      541 agggaattgt cccgggtatt caggaagctg ccgtagaaac ccgcggtgat ctggtacttc
      601 tcgttcgtga agtccagggt cgcctcgaac tgcttgtggg tctggccgat gggctcgggc
      661 gagaacagga agccgctgta gcggttggcg gtgccgcccg tgctgccgtt ggtgcccgcc
      721 acgccgcgca caccgtccat gcgcaggccg ctcttctcct cgttcttgaa catgaaggag
      781 agcttcacgt ccttggccac gaacttgctc gcgttgagcg tcacgatgtc ccgctcggtc
      841 ttgagggtga cgtcgtagag gttcgggttg gtcagcgacc cgctggtgaa cgcgctgttg
      901 gcgtagctcg gctgggtaat ggtgttggtg ccgatgccgg acaccgccgt gctgatggcg
      961 tagggcgcgt agcgcgggat ctggtcgtag ttgaggccaa gggcccagtc gccctgcttc
     1021 tccatgctca cgccgaattc ctgggtgctc aggcccaggt tgcgggcgtt gaggcggaac
     1081 cactcggcgt cgtcgctgcg gcgcacgatg ttcaggtcca ggttggcatt gacgccctgg
     1141 ttgttgatcc cgttgtactg gcgatacagg gggttaactt cgttgaccca cggcacgttc
     1201 accgtcactt cggtcacgtt ggggctgatc agctgggcga cgtcgtcatc ctccgcccat
     1261 gcgctgctca ggggaaacac ggccgacagg gcgataacga gggcggtctg ccggaattgt
     1321 tgctgggtgc tcatgatcat cctcctcgat taccggaaga agcgggtcgg accgctgctc
     1381 gtcgcattgc tggggctgtt gctgccgtgg atgttggtgt ggcagttcat gcagccgcgc
     1441 gcctgggcca tgcccggacc gatgttggtg ctgaccgcgt tgggcatcag gctgcgcacg
     1501 ccgggcacgt tgccggggtg ggagctgtcg ccgtggcatt gctggcagag gaagggctga
     1561 cgggacttga gcatgccttc ggccgtggtg ccgtgggggt tgtggcagtt gccgcagtcc
     1621 tccaccacgg gctggtggtt atgcacgaag gggccgcgct tctcggcgtg gcaggtgaag
     1681 caggtggcgt tggtggtgtc cttcaccagc atcttctgac cggccgaacc gtgggcattg
     1741 tggcagtccg agcaggtcat cttgccttcg gggatcgggt ggtgcgaggg cttgttggcc
     1801 tcggcgcgct gctgcttgtg gcaggtgtag cagacttccg cctgctggcg cttgtcgcgc
     1861 accttgtcct tgggcgagtg gatctggtga cacgaggtgc acgacacgtc ccggttggcg
     1921 tgggcgctgg tgttccagtt gatgtgcttg ccgccctggt gacaggtggt acaggccgcg
     1981 ctcttgtcct cggcgctgag cttggacagc ttgccgaagt tgcggtccgt gggcggacgt
     2041 tccttcagct tggccgggtt gttggtgtga aggtcgcttt ccccgtggca gttggtgcag
     2101 ctcggcgtgc ggccgtcggc cacggagccg tgcttggtct tgccgatatg cagcagcttg
     2161 ggttcatccg tctcgtcgtg gcaggacgtg cacttggcgt cccccttgag caccagatcc
     2221 ttggctgccg gcttggcggc tggcgtagcc ggtgcatcgg ccgccaggct tgcctgggcg
     2281 gccaccagtg cgccgaacac ggcacacgac gcgaacaact tacggaaaat tttcatgatg
     2341 atctccctag tactgctgca tgaccggcgc cgcgctccgt cgcgacaccg ggtcgttcaa
     2401 acgcgggcta cttgccgagc gacaggatcc agtcggtcag attcttgatc tcggccttgt
     2461 ccttggtctt gaggatcttg tgctcctctt ccttgccatc ctcgaacttg accatctcgc
     2521 cggacgtgag gtgatgcagc agcttggctt ccgcatcggc cttgcccttg agggacttgg
     2581 agatctcggt cagggacttg gcttccttct tcttgtccac ggcgtggcac ttggtacagc
     2641 cttccttctt ggccagggcc tgggcggcgt cagcgtccag agccagggcg ctgccggcgg
     2701 acagcgcggt cgccagggcg gcggtggcaa gacagacggt acggatcttc atcggggcgc
     2761 tccttgagaa cggaatgttg tgattgggtg aaacggggtc agtgttcgta ccagccggtg
     2821 atcatcatct tgaacaggct gctcaccttc gggcccatgg tgcccaggta gatgtggctc
     2881 accatgaaca gcaggatcac cgccgccgcc aggtaatgca ccagggcgat gggcagcagg
     2941 ccgtccatgc cgaagatctg gtccggcgcg aactggggat acaggaagat caggccggtc
     3001 acgatcaccg tcggcacgac gagatacatg atcgaccaat aggtcacggc ctgaagggca
     3061 ttgaagttca cttcaggcgt cggcgggaaa ggctcctctt cgcccttgaa gatgccgctg
     3121 ccgtagaact gcatctgctt caggcagcgc cccagcacgc cgggcggctt gggcacgtac
     3181 tgccaccagt tgccggtgac gatgttgccg atcacgaaga ccgagtacag caccgccagg
     3241 cacacgccgg ccacgttgtg gatgcgggcg gccatggaaa actccaccag ggccagttcc
     3301 gagcccgaga agtgcaggct gatgcccgta atggacaggg tgatgatcgc cagggcattg
     3361 ccccagtgcc acagcctgat ccagggggcg agcatgtaga cgcgcttagt catttttctt
     3421 gctccggcgg atgcgggtcg tgatgatgcg cagcagaccg tggcccagca caccgagcac
     3481 ggtggccacg aaggcgccga tgagcagcag atccagggtc gggttgcggg tcgcccccac
     3541 cacataggaa ttactcagga tcacgctgtt gaggaagccg tatttctgct gctcttcctt
     3601 ggccagatgg cggtagagcc gcaccttgag ggacgagtcc gggttgtggc aggtcacgca
     3661 cttcttctcg gccttgtcct tgttctgcac ctcgtgggac agggagagct tgtcgtcggt
     3721 gggggtgtgg cactcgatgc agcgcaccgc ctgccagtgc aggcgcgtgt tgggcagcca
     3781 tttgtggatg tcgtcgatgg ccgggcgctt cttcttgtcc ggcgccatct ttgcgaaggc
     3841 ttcgtcgttg tcgtggcagt ccaggcagat cttgttgtcc tgggccacga tcttgtgggg
     3901 atcgcggagc ttggccgcga agctcatggt gtgggcgttg tggcaggtgg cgcagctgaa
     3961 gcggtcgctg aggttctccg catggaccga cttgtcgaac tggcgctcga tgcgcatggc
     4021 cttgcgggcg tggcagtcct ggcattcggc caggtcctcc ttggccttgg gagcatgggg
     4081 atgctcgtcg aagccgtcgc cgtgacactt gctgcacgcc atctcgccgt gatcggatgc
     4141 cgcataggta tccggattca ccagcagcgt gcgcagcttg gccagatcca tgccctcctt
     4201 gaccggcgtg cggagcccgg cttccgagtg acacttgaaa cactccgcgt tggcggtccg
     4261 gatcttcgcc agctcctcgg gcgtcggggg cgcatcgtcg gccatcgcca gggccgcaaa
     4321 acccagcagc agcaggccca gcgcgccccg cacacgattt ttgaattttt tgaacacgtg
     4381 aattcctccg taggggtcgg accgattaga tcacccgaca cccggcggga caatttcaaa
     4441 ggggaaatca ctacccaaaa ttgacgagcc cttgatgtac atcaacccaa aacgggcacc
     4501 gtgcagcctt tttgcgacac ccatttgacg gattgccgca gcgtcaattg gcggagaagt
     4561 ccagcccgcg ggctattcca gcacgtccac cggcatcccg cagaccagct cgccggcccc
     4621 gtccgcgatc acgttctgcc cgaacagcac gccatccgca gtcttgcggt agcgggccag
     4681 ggtgcgcagc ggctcctggt ccaggctctt ctcgccggtc acgggatcga cggtcgtgaa
     4741 cacgcagcgc tcgcagggtt tgacgaggcg aaagctcacc gtcccgatgc ggatgcgcgt
     4801 ccagccatcc tccgcaaagg cctccgcccc cgacaccacc agattcggac ggaagcgcgc
     4861 catctccaga ggccggccga cccgggccga cagatcgtcg agggaggact ggccgatcag
     4921 cagcagcgga aaaccgtccg cgaaggacag gggcacgtcc ggctcgacac gcacgcgccg
     4981 cgccgtctgc gcgcccgtcc acaagaaacg caagggcgcg cccagggcgt cggacagcca
     5041 ggcatccgcc gccgccgcac cgccccaggc cgggaacagg ctgccccaca ccaccgccga
     5101 cacctcctcg gtgaagacct cgtttggcac gaacaaggcc tcgcgccccg ccatcgtcag
     5161 cgtgaccccg tcggccgacg gctccgtgcg cacccgcacc agctggggca gctggcgccc
     5221 ggtgacgaag cgcccgctgc gatcggccac catccacatg cggtcgaagg gcaagcccag
     5281 ccgcgacacg gcgctggcgt tcagggcgtc ccccgccatg gacttgacgg gatagcggta
     5341 gagggcggac aggatgggtg aaggctgggt catgacggca cacggctcgc ggtggaaggc
     5401 aggagcgacg gattatccct gccgcatcgc ccgccgtgca atcggccacg gccccctgtt
     5461 tcctgcgtca cgaaacggtg tgtaaaacgc cagcagcacg ttcggcaatc gcggtaacct
     5521 gccattccag cccacgccgg accgtcccga gcggttctcc agttcgatgc cagccctgct
     5581 ccgtgccctc ttcctcctca gtctcgccct gtgcgcccgc gtggcgtccg ccgacggtat
     5641 gcgggatctg gaaatcggcg tgctgcccaa tctcagcacc cgcgtgctca tgacccagta
     5701 cgcccccctg cgcgacgccc tggcgcgcag cctcaaccgc cccgcgcgca tcgccacggc
     5761 gcccgactgg tcgcgcttca cccgtcgcgg gctggaaggc gactacgacc tcatcgtcac
     5821 cgccgcccat ctggcgcgcc tgcagcaggt cgaggccggc ctgatccccg tgggccgctt
     5881 cacgcccgac atcccggccc tcctggtcac ccgcggcgat gcccccgtga gccgcctgac
     5941 cgatcttcgc ggcaagatcg tggccctgcc caacgtccat tccctcgtcg ccctgcagag
     6001 cctgcaccgg ctcgccgaag ccggactgcg tccgggccag gacctgcgcc tgatcagcgt
     6061 gcccaccgac gacagcgtcg gcaacctgct cctgcgtggc gacgcgaccg cagccgtgct
     6121 cagcggcggc gagttccgca tgatccccga agacatccgt gcccgcctca aggtcgtcca
     6181 gaccgtcgcc gaggtgccgg gcttcatcgt cctcgcctcc ccgcgcctgg acgcggacgc
     6241 cctcgaacgc ctgcgtcgct gcctggagag ctttccgggc agcagcgacg ggcgacgctt
     6301 cgccgagctg accggcttca gcggcctgac cacggtggac gacgccctca tgaagtccct
     6361 cgacatccat ctgccggacg cccgccagat cctgcaggca ccatgaacgc cctgtggccg
     6421 gcctccctcc gcttccgcct gctggtcgcc agcctggtgg tgggcagcat ctccctcgcc
     6481 ctgctcatcg cccacagcgc acgcctcatc gaggccagcc tgctcggcca gctcaaccgt
     6541 caactggaac agacccgccc cctcctcaac gccgccctca acgcccctct ggccgagcgg
     6601 gactacgcga ccctgcgcca gatcctgcac gaagcccgca cccccgaaat gctcacctac
     6661 ctggtcctcc tcgaccaccg cggcgaggtc atcgcaagcg agggacgccc ggccagcaca
     6721 ccgctccccg ccatcgatcc gcccgctcag tttgacttgg accgccccga ccgggtcttt
     6781 cataccgagg tgcacatcga tctcgccggc caggccctcg gcaccctgcg cttcgggttg
     6841 tccacgcgtt tcctgcacga cgaacgccag cgcctgatcc gcgaaagcct ggaactggga
     6901 gccgccgtgc tgctggtggc cctcttcgcc ctgggctggg tttccctgtg gctcacccgc
     6961 cgcctggccc gcctcaccga cgccagccgc cagctggccg tcggccatta cgacgtggag
     7021 ctggagagca gcggctccga cgaggtggac caactcagca ccgccttcct gcgcatggcc
     7081 gccaccatca aggtccgcgt ggaggaactg gtcctcagcg aggccctgca gcgccgctac
     7141 tacgaacacg cccgcgatga acgggcgcgc ctggcctccc tgctgagtgc cctcaagacc
     7201 ggcatcctgt tcgtcggcaa cgacggcatc gtcgtctact acaacgccac cttcggccag
     7261 atctggcgca tcccacccga agccgggctg gtgcatcagc ccgccctgcg cctgcgcccc
     7321 tacgtcgagc gcctcctcga cgacaccggc agcttcatgg ccatcccgct ggcggacgcg
     7381 gacctggccg aattccgcct cctcgaccac cgcatcgtca cccagaccac ctccccggtc
     7441 ctcgacgaga ccggcacctc cgtcggccgc atctggatct acgaggacat cacccaccag
     7501 cgccagaccg ccgagctggc cgaccaggcc gagcgcgacg ccctcaccgg gctctacaac
     7561 cggcgcggct tcgacctcgc cctcgaccgc atggcccgca ccgcccgcga acgcaacgcc
     7621 aacctggccc tgtgcttcat cgacctggac ggcttcaagc gcatcaacga ctgctacggc
     7681 caccgggccg gcgacgccat cctggtgcag gtggcgagcg agttccaggc ccaggtgcgc
     7741 cgccgcgaat gcctcgcccg cgtcggcggc gacgaattcg cggtcctcgt ccccgacgtc
     7801 agcgaagagg agatcaccct cctcgccgag cgcctcgtca aagccatcgc cggcatgccg
     7861 atccgctaca ccgggcagga cctgcgcctc ggctgcagcg tcggcatcgc catctacccc
     7921 tggcacaccc gccagcccga tgacctgatg gcctgcgccg acgccgccat gtacctggcc
     7981 aaggacgccg gcaagaaccg ctggtgcatg ctccccaccg caccgccccg tcacgccggc
     8041 gacacctgat cgtcgcggcg cggtgctaca gtacgcgggc tgcatgttcc gcggagctgc
     8101 catgcccccg acccgcgccg ccctcgcggc caccgccctg atcgtcctga tggcgacggc
     8161 ctgtgccgac gcaccgaccc gcaccgcgac cggcgtgcgc atcctcgtcg aattcaccac
     8221 cccgaccgac ggcgccgcgc cagccacgcg ggccttcctc gaagccacgg gcggtgtacc
     8281 ggtccgctac gtgacggcgg tgtccgggcg gacccacgcc tatcgcctcg cctgcccgcc
     8341 ccaggacgac acctgcagcg ccgccctcga cgccctgcgc cgcgcgccgg ccatccgcag
     8401 cgtcaccccc gaccacctca aggacacccc atgacgtcca ccctccgcgg gacctgcctg
     8461 gccctgctcg ccctcaccgg cctgagcctg ctccctgggg cggcccgcgc cgacgacgcc
     8521 gtcggcggcc gcgtcatcgt caagttccgc agcagttccg ccctcaagca gatcgccgca
     8581 gccggtcgtg tccagagcct ggcccagcgc cttggcgtga cggccaccct catcgggcag
     8641 cctgcgccgg agctccacgt gatgcacgcc cgcggcatca gcaccgcagc cctcgccgcc
     8701 cgcctcgccc aggagagcga cgtggagttc gccgttccgg accgcctcaa gcaggccgcc
     8761 gccctgccca acgaccccct gttctccgcc cagtggatgc tgcagacgcc cgacaccacg
     8821 caccccgccg ccatcgacgc ggtcggcgcc tggacgctca gcgcgggcag cagcggcgtg
     8881 gtggtggcgg tggtcgatac cggcgtgcgc ctcgaccacg aagacctgat gagccgcctg
     8941 ctgccgggct acgacttcat cagcgacgac ctcaacgccg gcgacggcca acccggccgc
     9001 gacgccgacc ccgcggatcc cggcgacttc gtgagcagcg ccgacctcgc caacagcgcc
     9061 ttcacccagc tctgtcccgg cgtcagccgc gccagcagct cctggcacgg cacccagatc
     9121 gccggcatcg tcgccgccga agccaacaat tccctgggaa ttgccggcgt ggccggcgcc
     9181 acccgcgtgc tgcccgtgcg cgcgctgggc aagtgcggcg gcttcgactc ggacatcatc
     9241 gccggcatgc gctgggccgc cggcctcagc gtgcccggcg tgccggccaa tacccatccg
     9301 gcccgcgtca tcaacctgag cctggccggc agcggcagct gcagcgccgc ctacgccagc
     9361 gccatcgccg agatccgcgc ggccggcacc ctcatcgtcg ccgccgccgg caacgagacc
     9421 ggccccgtcg aagagcccgc caactgcgcc ggcgtcctcg ccgtggccgg cgtacgccac
     9481 gtgggcacca aggtgggtta ttccagtttc ggtgccgagg tgggcctcag tgccccggcg
     9541 ggtaactgcg tcaatactgc gaccggttcg ccctgcctct tccccttcgt gaacgccacc
     9601 aacctgggca gcaccgtccc ggctgccaac ggctacaccg gcctcaccgc ccagaccgtc
     9661 ggcaccagct tcgccacccc cctggtggcc ggcaccgcgg ccctcatgat cgccgcccat
     9721 ccgggcctcg gcgtcacggc cctgggcgag cgcctgcgcg cctccgccac cgccttcccc
     9781 agcgacgcca gcctcgccgc ctgccccacc acgaccgccg acgggcagtg caactgcacc
     9841 accgccacct gcggcgccgg catgctcaat gccaaggccg ccgtgcaggc cgcacaggcc
     9901 cccgaggcgg ccgtcgcggt gtccgccacg ccggtggccg gcagcgccgt caccctcagc
     9961 gccgccgcca gcaccaccgc acccggcgcc acccttgtcg catggcaatg gacccagacc
    10021 gccggcccgg cgagcgccgc cttcagcaat gcggcggccg ccagcaccag cttcaacgcc
    10081 ccgcaggccg gcacttacag cctgcgcctc accgtcaccg acgacgccgg gcgccagggc
    10141 agcgtcgaca ccaccctcga cgtccaggcc ccgcccccgc ctgccgaggg tggcggcggc
    10201 gggggcgcgg tggatggacc gctgctcgcc ctcctgggcg ccgccgccag cctcgccatc
    10261 gcggcccgcc gcggcgtccg gcgcccctga cccctgcctc ctccaacccg aaaggatctc
    10321 ccatgcgcta caccggaagc tgtcactgcg gccacgtcgc cttcgaagcc gaaggcgaga
    10381 ttacgggtct cctggaatgc aactgctcca tctgccggcg caagggcgcg ctgatgtggt
    10441 tcgtgccgcg cagcgccttc cgcctcctca cgccggagga gaacgccgcc acctacacct
    10501 tcgcttccgg ccgcatccat caccgcttct gcccccgctg cggcatccag ccctacggcg
    10561 aaggcgacga cccgacccac ggccccatgg tcgccatcaa cgcgcgctgt ctcgacaacg
    10621 tggatctggc cgccctgccg gtccgccact tcgacggcgc atccctgtag cgcacagagg
    10681 gccatcgcgg ccctcgccca tcccggcgcg agcgcgtcgc ccggcgccct cagatcaggc
    10741 gcgccggggg aatggcctca ccgccgggcg ctgcctcacc gcaccggatc gcatccgcgg
    10801 ccccgagcca cgcccgtgcc caggccaccg cctcccggcg cccggcctcc ggcagcggat
    10861 tgaacgcata gcgcagcccc atctcgcgca gcaggccggt gagccccccg cgcagcgcct
    10921 gcacggcctg catctgcgcg tgccaacctg cgcggacccg cgcctcggcc cgcgcgcgct
    10981 catcgcccgc acgcgagtcc gtgtcgcccg tcccgtgggg cccattgcca cggatccagc
    11041 cgggcacggt ctcgcccgcc agacggcgac cgccctcgat caatacctgt ccgtgtccgc
    11101 ccaggcgggt cagcatcccg gccatccgcg ccagctccac ccgggcgtgg cacagcagac
    11161 gcgccgcggc cgcctcgact gcggcgccgt cggccgccat cgccagcgcg ttcagatcgg
    11221 cgttcagcgc cgcctcatgc gcggcctccg cgaacgccca cgccccccca tcgccgccct
    11281 cggcgtccgc ttcgcacacc gcgggcaacg ccccggaggg tccatcgtgt gggggcgccg
    11341 gtccttcgcc cgcgccgacg gcgggcccgg gccgggccgc ctgcgggaca ggcaccggtg
    11401 cgctcgcctc ctccgcgtca catcggatcg gccgccgctc cgacgtcagc cgggcagcgc
    11461 ctgcgcctga ttcatcgtcc gcccatccgg gcgccacgtc ggcgcggcat tcaaacacgc
    11521 ggccacgggt gtgagcctcg cgcgaccagt ctgcgtcctg cggagcatag acctccgcca
    11581 ccgctgccag cgccgcatcc acggcctcgc gcacggtgtc cagctcgccg cagaggccct
    11641 gcacgccctg acgcatgcag tcgaggtggg gcgtgcagcg cgccaggcgc agcagcccgt
    11701 cctccggata gccggccgcc gcatccttca gcgccgcgtc gacagcgctc atgaatcgcc
    11761 ggccgccgcc ctcgaagagg gccagcgcgg cctccagatc gccggtccgc tcgcgcagca
    11821 gatactgcac ccgcatcacg cgcaccgcga gggcgacctg tgtgcgggga tgacagaccc
    11881 cggcgagccc gcaggccgcg gcgtggagca actcgctcag gcgatggatg gggtccagcc
    11941 agcgcgcgag ctccacgtcg ccggcccgcg caccgtttac gccgtccgcg cccctgacgt
    12001 cgccccgcgc gcccttctcc gccgcctcgc cgatgcgctg cgtcgccccg tcaggcccag
    12061 cgcatcccgg cgtgcccgcc tcatccacca tggcgatcca cgatcctccc ggcggccggg
    12121 gcacggcctc gtgcccctcg atcccgctca ggcgccatcg gccagcagga tgttgcggcc
    12181 atcgtcgatg ctccgggcga agtccttcag cgtgtcctcc aggctgcgca ccgtctccgc
    12241 gtcgtagcgg tcgaacaccg gcgtcggggc ctgcgtgctg gcgcgctggg ccaggcgctg
    12301 caccgaatcc tgcagcatcg ccacggtgcc gatgttggac tggacgagca cccccaggtc
    12361 gcgccagaac tgcacggcgt ccgtcaggcg cgtcaccgcg gcccgcaggg tgttctgctc
    12421 ccggtccagc tggctgcggc gggcctgcag atctgccttg cgggccctca gcatgtcgag
    12481 ggaacgctgc gccctatcgc gctcgttgcc ggccccctgg cgatccatct cgatctggat
    12541 ccgctgccgc tggttgtcgg cggcctcgtt gttgagcgcc ttccagcgat tggcaaacac
    12601 atcggcgaag ctcagccgcc agcactccca gctgtagaac atcccctcca gcggttgcgc
    12661 cctgcattca tcgcgggtcc gcccctcctg gcggagctcg gccatccgcg cgtccagggc
    12721 gacgcgcacg cgcgccacct cggcaagctg cacgtccagg tccgcgatgc gcgcctgccg
    12781 gtcccgcatc tcccggtcgg cctcgccttc gaggcggaag gtctcgtcga tctcctggac
    12841 cgtgcgcagc acatccgcct gacgccggtc ggccgcaccg cgggcgcgat tcacataggt
    12901 ctcggtgtcc tgacgcagac cgtccgcggc gctgcggata tccgtcagca gcgcctgggc
    12961 gcgggaggtg ttgcccgccg ccagcgccgc ttccgactgg tgcagggcgt cgaagacccg
    13021 tgtgacccgc tggagctgcg ccccatccgc ccaggccagg ccccacagca gcagccacag
    13081 cccgaccagt ccgtgcaagc ttctcttcat cacgatgtct cccgcccgcg cccgacccac
    13141 gccacctcgc ggcggcccgt cacggccccg cgaagtggcg catcccgggc gcaggactgt
    13201 gccgggtgtc aggcggccgc cgccttggcg atcagcttgt ccgcctctgc cttggctgcc
    13261 gcaatctgct gggtggcgtc atccgtcttc ttgatctcgt ccttcacgtc cttgtccagc
    13321 agctgggcga gccgcttggc ctcggcgcgg gcctgttcga tgggcaggtt gtcccggtag
    13381 gtgacgccca gcttgtcgaa gacctgcatc gtcgccgccc ggtattccgt cgccacccgt
    13441 ttcagggcgc gccactgggc ggccaccgcg agcatgcgca gcttgaaatc ctcggcggag
    13501 tattccttca cccgctcttc cttggtgcgg cgcatgtaca cctcgatgtc ggtgcgcagg
    13561 tccgcactcg ccagccgccg gcaggccgcc gccatctggg tccagaactg gcagacgtcc
    13621 tgcaggatca ccaccacgta cttgagcacg cccaccgcga tgtgcagcga ttccaccgcc
    13681 gcctgttcga gctgctcggt gtccttctcg gtctgcatct gcacggcaaa cttggcgatt
    13741 gcgcccagcg cctcccgctc ctgcttctgc aggtccatca gcatgtccag gtacttggcc
    13801 ttttccttgg cgtagttggc ggcgatgtcc gcgtaggagg cgcccatctc ctgggcgctg
    13861 gcacccgccg aacgaaggcc ctccgccacc cccgcgaggg gcgccttggc ggccgcgtcc
    13921 tccatcaccg ccttggccgt gctctccttc accttggcgg cgtccaccgc cttctgggcc
    13981 gcctcctcgg ccgcctcggc gtcttccact gcgtcctcgg cttccttgta gggctcttcc
    14041 agctcctcga aggcctcctt cttggcggcc tcgtccttct tgtccttggg cttcttcttg
    14101 cgcgccgctt cataggcgtc gcgggcctcg atgtagggct cctccagctt gtccagcttg
    14161 ccctgggccg tcttggtcgc cgtctgggcc gcggtgagct tcgcctccgc ctggtccagg
    14221 gcgtccttcg ccacccggta ggcggcctgg gccgtcaggg tctcggaggt ggccttggcc
    14281 ttgtcgtcgg tcttcttgtt cttctcgtcg ggctccttga ccgccgtgtt cttgacgatg
    14341 gccgtcgcca gctgcgtgcc cgccgtcacc ggcgcggtct tgaactggag cccggccgcc
    14401 agcccgtccc ccagggcctt ggtgatgctg cccacgatgg ccagggcgaa catgcgcccg
    14461 tcggcggtgt tctgggccgc cttggcctct tcgtaccagc ccttcacctg ggggatctgc
    14521 tccgcgaggg aggccttcag gcgctccttt tcttccagat cggccttgat ctccttctgg
    14581 cgcgcctggg cggcctcctt cgccttgcgc cgcagatccc gcgtctgggt ggtgtcctcg
    14641 gcgatcttct gggcctcgtc ggcgagcccc ctgaaggtgc tcttgaggtt ctccgccgtc
    14701 gtcgccatgc tggtcgccac agcctcgcag ccggtcaggc ggcccacgca gtccgcttcg
    14761 tagaagccgt acagatcctt gaaggcgccc ctcaggatcg ggatcatccg ccccgccgcc
    14821 tcgccgaagc tgagcagcgc agagctcatc tcaccggtct tgtcgcgcag cttgtactgc
    14881 aggttcatca cctgggcgct gagggacttg ccggactccg gattgaggaa gcccgccacg
    14941 ccgcacgcgg cgatgtgcag cagatcgtcg gtgcggttga ggttgcgcac cagcttgtcg
    15001 atctcgaggg tctgcaggat ctgggcgtgc gccgcctgca ccaggggcga actgtcgtcg
    15061 gggccttcga tgacgttata cgtcatggtg tcgaagacga tgacgggact gggcggggac
    15121 accacaggcg gtgtggccgg cacgggatcg gacatgggtg gactccggaa gacaggaaga
    15181 aggaagagac gccggcgagg tcccccgggc actgcaccca ggccattccc cgccggcagg
    15241 gcggccacgc cgggccgcag acagccgggc gccgtcagcg ccctttcttg tattttcgga
    15301 cgagggcctt gatgtaatcg atgatctgct gctcccgctc ggtgggatct tccttcaggt
    15361 cgtcgatgac cttgatgcag gccggctcgc gcaactcgtt gccatgcagc agcttggtgt
    15421 ggtgatgccg ggtgtcggtg aagtcgatgc cgccaccatg ctcgtggata tgcggctcac
    15481 ctttgtccgg atagaacttg tctcccatga cgcgctcctg agtggggttg agggtgtgtg
    15541 tctttttgtt aggaatgaat gcagtgtaga cgccatcaat cggaatacaa gcatgacaaa
    15601 gcggttcaca taaaatccca aagcgctcac ccagcacccc tgcaattcgt caaaaaatat
    15661 atttatttca atagcttgaa aacatgccac ggacagcgcc cacaccccga gcgccagcgc
    15721 agcctccgca tccgccgcat ccccctcccc ttaaagccca ttacccccca cgcatccgcg
    15781 ggagacccct tcggacggtt cgcccg
//
LOCUS       DAOQMM010000089        15772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1453_length_15772_cov_51.152828, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000089 DAOQMM010000000
VERSION     DAOQMM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15772
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1453_length_15772_cov_51.152828"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLH85_13200"
     CDS             complement(<1..194)
                     /locus_tag="PLH85_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNB65569.1"
                     /translation="MTTKAQALLDWVDARFPLTSSIKGHLTEYYAPKNFNFWYFFGSL
                     ALMVLVIQIVTGIFLVMHYKP"
     gene            complement(198..791)
                     /gene="petA"
                     /locus_tag="PLH85_13205"
     CDS             complement(198..791)
                     /gene="petA"
                     /locus_tag="PLH85_13205"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNB65570.1"
                     /translation="MSVDQKVDASRRQLLLVTSAAGGVAAAAAAVPFVASLTPSERAK
                     AAGAPVEVDISKLQPGEMMTVEWRGKPVWIINRTKEMLDSLKKTDAEVSDPNSDKPQQ
                     PEYAKNETRSIKPEILVAVGICTHLGCSPSSKFKTGAESGVSADWPGGFLCPCHGSTF
                     DLAGRVYKSKPAPDNLEIPPHKYLADTKILIGEDGKA"
     gene            1041..2453
                     /gene="dacB"
                     /locus_tag="PLH85_13210"
     CDS             1041..2453
                     /gene="dacB"
                     /locus_tag="PLH85_13210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNB65571.1"
                     /translation="MPAPFARFLFLCLLSGFVRAEAVLPAPVQEALNRASIPRDAVAV
                     WIQDLEAPRPIVTHRVDAAMNPASVMKLVTSQAALELLGPTYVWRTRITSTAPLRRGV
                     LDGDLAITASGDPRLSRERLWLLLRDLRMAGVSRIAGDVITDRSVFSLPPYDPGAFDQ
                     RPLRPYNAPADALSVDYSALRLRISPTTDGAIVRSEPLPAGLSLVSRIRRNAGPCADP
                     SAGLSAGVEIADAGTRLVLDGFVPQDCTAPFDWNVAPLTPARLFEGLFRSLWAEMGGT
                     LDGRFRDGPTPDGGAVLAESVSPALPEVLRDMNKWSNNVIARQLFATLGAVTEPGADS
                     LAAGARACRTALQAAGIDTRTLVMENGSGLSRSERASAALLGQLLIRAWHSPTMPELI
                     ASLPIAGRDGTARRRLAGSTTAGFAHVKTGTLDDVRSLAGYVLARNGRRYGVVLLINH
                     PNASAARGAQDALLEWVANL"
     gene            complement(2466..2840)
                     /locus_tag="PLH85_13215"
     CDS             complement(2466..2840)
                     /locus_tag="PLH85_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HNB65572.1"
                     /translation="MPVSDALNVLGGPLLACSYDPLTGWMRTGCCEADPADLGRHHVC
                     VKVTAEFLAFSTRRGNDLTTPKPDYRFAGLKPGDRWCLCAFRWREAWEAGVAPPVVLE
                     ATHAATLEVIPLEALKRHAYPR"
     gene            complement(2852..3871)
                     /gene="galE"
                     /locus_tag="PLH85_13220"
     CDS             complement(2852..3871)
                     /gene="galE"
                     /locus_tag="PLH85_13220"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNB65573.1"
                     /translation="MPTILVTGGAGYIGSHTCVELLAAGYDVVVVDNFSNSKPEALKR
                     VEAIAGRPLAAFYRSDIRDKAALREIFRTHAIDAVIHFAALKAVGESVAKPLMYYDNN
                     IAGTVALAEAMSDAGVKSLVFSSSATVYGDPASVPIREDFPTGPTNPYGRTKWMMEYV
                     LSDLAAADPAWRVALLRYFNPVGAHASGRIGEDPNGLPNNLMPFVSQVAVGRLPRLRV
                     FGGDYATVDGTGVRDYIHVVDLAVGHVAAVRRLTERPGVLTVNLGTGRGYSVLEVVQA
                     FERACGRPVPYDIVDRRPGDVAQCYAETTLAAQELGWKAERGIDEMCADAWRWQSSNP
                     QGYPD"
     gene            complement(3944..5134)
                     /locus_tag="PLH85_13225"
     CDS             complement(3944..5134)
                     /locus_tag="PLH85_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNB65574.1"
                     /translation="MDKPLQGVKVLELGTLIAGPFCTRILAEFGADVIKVESPDGGDP
                     LRQWRKLHEGTSLWWAVQARNKRSLTANLKHPDGLAVVRRLAAEADIVVENFRPGVLD
                     RLGLGWEALSAANPGLVMVRLSGFGQTGPYKDRPGFGAIGESMGGLRYITGFPDRPPV
                     RTGISIGDSIAALWAAIGALMALRHREVNGGAGQVVDVALYEAVFAMMESMVPEFDLF
                     GFVRERTGNIMPGITPSNTHTTRDGRHVIIGGNGDAIFLRLMQAIGRPDLAGDPTLAS
                     NAGRDARAAELYAAIDAWCTAHDGDAVLAALEAAQVPASSVFSVADMFRDAQFLARGM
                     IESANLADGTPLRVPGIVPRLTGTPGTTAWLGPTLGEHTDAILADLGYTPAAVTALRE
                     NGAI"
     gene            complement(5178..5774)
                     /gene="recR"
                     /locus_tag="PLH85_13230"
     CDS             complement(5178..5774)
                     /gene="recR"
                     /locus_tag="PLH85_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNB65575.1"
                     /translation="MSSPSSLDTLIEALRCLPGVGPKSATRMAYHLLQRDQRGAARLA
                     TALGHALEVLRHCTRCNTLCEDEVCARCANPRRDGTQLCVVEMPADLAMLEQTHSYQG
                     LYYVLMGRLSPLDGIGPRELHLEKLLARATDGVVQEVILATNFTNEGEATAHMISELL
                     RSRGLRVSRIARGIPVGGELEHTDTGTIAQALVERRAL"
     gene            complement(5791..6114)
                     /locus_tag="PLH85_13235"
     CDS             complement(5791..6114)
                     /locus_tag="PLH85_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNB65576.1"
                     /translation="MMKGGIAGLMKQAQQMQENMKKMQDSLGGIEVEGQSGAGMVKVT
                     MTCKYDVRRVSIDPSVMDDKEMLEDLIAAALNDAVRRVESTTQEKMSGFTAGLNLPPG
                     FKLPF"
     gene            complement(6136..8064)
                     /gene="dnaX"
                     /locus_tag="PLH85_13240"
     CDS             complement(6136..8064)
                     /gene="dnaX"
                     /locus_tag="PLH85_13240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNB65577.1"
                     /translation="MAYQVLARKWRPRSFETLVGQEHVVRALTHALATGRLHHAYLFT
                     GTRGVGKTTISRILAKALNCETGVTAAPCNQCAACVAIDADRFPDYVEMDAASNRGVD
                     DMAALLDQAVYAPVQGRYKVYMIDEVHMLTGHAFNAMLKTLEEPPEHVKFIIATTDPQ
                     KIPVTVLSRCLQFNLKQMPPGHIVGHLTRLLEAEAVPFEPGALRHLAKAAGGSMRDAL
                     SLLDQAIAHGAGKVEEQAVQDMLGTVGDDHLYAILDALVAQDVQGLMAAAEGMEARSL
                     SFDSALQSLASLLHRIALVQLAPTAVTDEFEAARLAPYAEAFDAEFLQLAYQIAIHGR
                     DELPLAPDEATGFSMSLLRLFAFRPEQPPALGSAAASGGGGTGHTRPLPAATSRGAPA
                     ERPAPSPALSTATPAALRTAPAPTPLPVVAQRSAVPVPQHATQVPAVAPAPEMGSSVP
                     PWEDLPPEAHGFAAAPILEVPPALPVAEAPARPAEPAALPSAVAPDRPVEASPAELPS
                     GDLPPLPAGLDWHALLADLGLGGMVRELAQHCELTGTTDGTLRLRLGHTHRHLLAINR
                     SGPEKLQEALAAHFGRPLRVHVEVGEIATETPAQRNEAVRAERHAAAVAALEQDPFVR
                     ELIERFDATMEESSVKPL"
     gene            complement(8153..8536)
                     /locus_tag="PLH85_13245"
     CDS             complement(8153..8536)
                     /locus_tag="PLH85_13245"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HNB65578.1"
                     /translation="MTSDPSDSERRKRRDSEIRCQDEAIGLHLAEALRSGELQTAASF
                     GKPMPEPEGWSETPGEFRMAFKILKNAGAPPPEIDLFHQRARLRAELEAASSDARRQA
                     IRQRLSELEQALALRLEGMRVSGKL"
     gene            complement(8579..8827)
                     /locus_tag="PLH85_13250"
     CDS             complement(8579..8827)
                     /locus_tag="PLH85_13250"
                     /inference="COORDINATES: protein motif:HMM:NF018599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNB65579.1"
                     /translation="MLELRPGCECCDRDLPPDSAEARICSFECTFCATCAEEVLGGRC
                     PNCGGELVARPRRPAEKLATHPPSDRRVRRPAGCGTQD"
     gene            complement(8932..9315)
                     /locus_tag="PLH85_13255"
     CDS             complement(8932..9315)
                     /locus_tag="PLH85_13255"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNB65580.1"
                     /translation="MAIYHGSCHCGAVRFAIDTEIIRVTECNCSICRKKGILHHRVAP
                     ERFSLLSGEEVLGTYRFGSGVARHHFCTVCGIHVFTRPRAAPELYTVNVRVLDDYDVT
                     ARQPEVVPFNGREWEASVAALNAPR"
     gene            complement(9569..9667)
                     /gene="ffs"
                     /locus_tag="PLH85_13260"
     ncRNA           complement(9569..9667)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLH85_13260"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9714..11144)
                     /locus_tag="PLH85_13265"
     CDS             complement(9714..11144)
                     /locus_tag="PLH85_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65581.1"
                     /translation="MKLGRLFWKFFFAFWLALLLAGVGVGAAVWWHHQADLEQRERER
                     DPALAGGPRAGWAVASAAAVLAHGGTPAVLSLLDEWGAEGRAPRLLVVDEAGHDLLGR
                     PVSAEALASARRRADHDHPAAPVRQVLSADGRTYLLFVPADAGNRGWEGVPPGPPPGL
                     DLPGRPPGPPFERRRPPDRPGLGVLIASGLLASVLVSALLAWYLAKPVRHLRWAFGAV
                     ADGRLDTRVSPLMGARRDEIADLGVEFDRMAGQLQALVGAQRRLLHDVSHELRSPLAR
                     MQAAIGLARQDASKLPATIDRIERESVRLDELVGQLLTLARLDAGSGDAPEEAVDLID
                     LVAAIADDARFEAETLGKTVHFVGDGQALTRVRAELIQRAFENVIRNAVKFTAVGTAV
                     DVTAGVIDGRFRLRVSDRGPGVSESDLATIFEPFHRGRAAVTAGGFGLGLAIARRAVT
                     SHGGQIEASNRDGGGLEMSIVLPLAG"
     gene            complement(11141..11833)
                     /locus_tag="PLH85_13270"
     CDS             complement(11141..11833)
                     /locus_tag="PLH85_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB65582.1"
                     /translation="MNRVLLVDDDVELSALLREYIEQEGYEATVVHDGESGVAEALSG
                     RHAIVVLDVMMPGIGGIEALRRIRAQSNVPVLMLTAKGDDVDRIVGLELGADDYVPKP
                     CTPRELVARLRAILRRTHEPASSADSPNGTVAVGPLLMWPQMRRAEWQGRALELTSTE
                     YNLLELLARNAGRVVSKTELSEQGLGRPLARFDRSIDVHLSSIRHKLGARADGGSWIE
                     TVRGLGYQMIRE"
     gene            12019..13530
                     /locus_tag="PLH85_13275"
     CDS             12019..13530
                     /locus_tag="PLH85_13275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNB65583.1"
                     /translation="MRRIIAHRPRHLALALLGSLMLSGPLRAANLPDLGDASEATLTR
                     QAERTLGEQTMRSLQASGALLDDPEVNAYLDSLGRRLLQADPSLAGQPFDFFAVESSE
                     LNAFALPGGHVGVHTGLILATQSESELASVMAHEIAHVTQHHIARQIAGQSGAQLASM
                     AALGAAILAAATGNGQVAMAAVTGATAANLQSQINYTREHESEADRIGFQLLTGAGFD
                     PRAMASFFDRLQKATRLVDGNVPSYLRTHPLTHERIAEAEDRAFNLSYKQVRDSDEYH
                     FVRALLRSYEGEPREAVALLDKALAEGRTQNARATRYGLTAALLRAGDFKRADKELAV
                     VERDGPHPMVEALAGQILLQSGRLPEARKRYESALQRFPDHLQLVYDYPRVLIRLNDE
                     AAAARFTESRLLARPQDPTLHRLAAEAFAALGQQTRSHVHQGEFEAARGNTQAAIEQY
                     RIALRSADGSERDLTIADARLRELQAQQRELRKDGRDGGKDPSGGRLHSFTPR"
     gene            13628..14164
                     /locus_tag="PLH85_13280"
     CDS             13628..14164
                     /locus_tag="PLH85_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNB65584.1"
                     /translation="MSSLFTNRFKTLLAVAGIAVATPFAAHAFGGHEHCGGPMMHREM
                     GFGGPEGGAMHLPPFVRELKLSDAQRDQIFKIMHEQAPAFRDKAIAARKAHEELRDMA
                     FSARYDEARVAALSDTEARAMAELTQMRLRTGNQIYQVLTPEQKKKADELKADFETRM
                     TRPVRDGGQPGKREPLRN"
     gene            14279..14737
                     /locus_tag="PLH85_13285"
     CDS             14279..14737
                     /locus_tag="PLH85_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNB65585.1"
                     /translation="MRNFDEIDRKILGILQRDGRITMTDLAPQVGLSVTPCTERVRRM
                     ERDGVIAGYHARLNPRALGLNALLFLELRLTSRSAEALTQMSETLAEIPDIMECHYVS
                     GDFDCLVKARLPNLGEYRPFIADLVERLPVVESRSYVVMEEIKESPLLNA"
     gene            complement(14861..15760)
                     /locus_tag="PLH85_13290"
                     /pseudo
     CDS             complement(14861..15760)
                     /locus_tag="PLH85_13290"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
BASE COUNT         2653 a         5660 c         5086 g         2373 t
ORIGIN      
        1 ggcttgtagt gcatcaccag gaagatgccg gtgacgatct ggatcaccag caccatcagg
       61 gccagagatc cgaagaagta ccagaagttg aagttcttcg gcgcgtagta ctcggtcagg
      121 tggcccttga tggacgacgt cagcgggaac cgcgcgtcga cccaatcgag caatgcttga
      181 gctttggtgg tcatcgttta ggcctttccg tcttcgccga tcaggatctt ggtgtcggcc
      241 aggtacttgt gcggcgggat ctccagattg tccggcgccg gcttgctctt gtagacgcgg
      301 cctgccaggt cgaaggtgga gccgtgacac gggcacagga aaccgccggg ccagtctgca
      361 ctgacgccac tctcggcacc cgtcttgaac ttcgacgacg gggagcagcc caggtgggta
      421 cagatgccga ccgccacgag gatctcgggc ttgatggaac gggtttcgtt cttggcgtac
      481 tcgggctgct ggggcttgtc ggaattcggg tcgctgacct cggcatccgt ctttttcagg
      541 gagtccagca tctccttggt gcggttaatg atccacaccg gcttgccccg ccactccacg
      601 gtcatcatct caccgggttg cagcttgctg atatccacct cgaccggcgc accggccgcc
      661 ttggctcgct cggacggcgt caggctcgcg acgaacggca cggccgcagc ggctgccgcc
      721 accccgcccg ccgccgatgt caccagcaag agctggcgcc ggctcgcatc cactttttga
      781 tccacgctca tggcccacta acctcgataa aagagacttg gaagatcgca atacggaaaa
      841 ctcgcggagt atagcgaaaa cccttacctg aaaaaagcct tatgggccaa agccgcgccg
      901 gatcaggcaa atttgggcgt ccgcggtgct gccaaaggcg cgccatcgcg gcccgtccgg
      961 ggcgctgtgt tgcggcgcac aatgctgcag gcgcgccctc ggcaggcata atcgtgtcca
     1021 ttgccgtgcg cactccgacc atgcctgcac cattcgcccg tttcctgttc ctgtgcctcc
     1081 tgagcggatt cgtccgggcc gaagccgtgc tccctgcgcc cgtacaggaa gccctgaatc
     1141 gtgcgtccat tccgcgcgat gcggttgccg tgtggatcca ggatctggaa gcgccacgcc
     1201 ccatcgtcac ccatcgtgtc gacgcagcca tgaaccctgc gtcggtaatg aagctggtca
     1261 ccagccaggc tgcactggaa ctgctcgggc cgacctacgt gtggcgcacc cgcatcacgt
     1321 ccacggcgcc gcttcggcgg ggcgtgctgg acggcgatct ggccattacc gcgagcggcg
     1381 acccgcgcct gtcacgggaa cgtctgtggc tgctcctgcg ggacctgcgc atggccggcg
     1441 tctcgcgcat cgccggcgac gtgatcaccg accgcagcgt cttcagcctg cccccctacg
     1501 atccgggtgc cttcgaccag cggccgctgc gcccctacaa cgcgccggcc gatgccctga
     1561 gcgtcgatta cagcgcgctg cgcctgcgca tcagccccac gaccgacggc gccatcgtcc
     1621 gcagcgagcc cctgccggcc gggctgagcc ttgtcagtcg catccgccgg aacgcgggcc
     1681 cctgcgccga tccgtcggcc gggctgagcg ccggcgtgga gatcgccgat gcgggcaccc
     1741 ggctggtcct cgacgggttc gtcccccaag actgcacagc gccattcgac tggaacgtcg
     1801 cgcccctgac gcccgcacgc ctgtttgaag gcctgttccg cagcctctgg gcggaaatgg
     1861 gcggcacgct ggacgggcgc ttccgggacg gtccgacgcc cgacggcggc gcagtcctcg
     1921 ccgagagcgt ctcgcccgcg ctgcccgagg tgctgcggga catgaacaag tggtccaaca
     1981 acgtcatcgc ccgacagctt ttcgccactc tgggtgccgt gacggaaccc ggcgccgaca
     2041 gtctggcagc cggcgcccgt gcgtgccgca ctgcgctgca ggccgcgggc atcgacacgc
     2101 gcacgttggt gatggaaaac ggctcgggcc tgtcgcgatc ggaacgggcc agcgccgccc
     2161 tcctcggcca gttgctgatc cgcgcctggc acagcccgac catgccggag ttgatcgcct
     2221 ccctgcccat tgccgggcgc gacggcacgg cccgccgtcg cctcgcaggc tccacgacgg
     2281 ccggcttcgc ccatgtgaag accggcaccc tggacgacgt gcgcagcctg gccggctacg
     2341 tgctggccag gaacggccgg cgctacggtg tcgtgctgct catcaaccac cccaacgcca
     2401 gcgcggcacg gggcgcccag gacgccctgc tggagtgggt cgcgaacctt tagcggggcg
     2461 gggtctcaac gcgggtaggc gtggcgcttg agggcctcga gggggatgac ctccagcgtc
     2521 gccgcatggg ttgcctccag cacgacgggc ggcgcgacgc ccgcctccca ggcctcacgc
     2581 cagcggaaag cacacaggca ccaacggtcg ccgggcttga gcccagcgaa acgataatcg
     2641 ggcttgggcg tggtcagatc attgccgcgc cgcgtgctga aggccaggaa ctcggccgtc
     2701 accttgacac acacgtggtg acgccccaga tcggcggggt ccgcctcgca gcagccggtg
     2761 cgcatccagc cggtcagcgg atcgtagctg caggcgagga gcggcccgcc cagcacgttg
     2821 agggcgtcgc tcacaggcat gtcttcggtg ctcaatcggg atacccctgg ggattggaac
     2881 tctgccagcg ccacgcgtcg gcgcacatct cgtcgatgcc ccgctcggcc ttccagccca
     2941 gttcctgcgc ggcgagggtg gtctcggcat agcactgggc cacgtcaccg ggacggcggt
     3001 cgacgatgtc gtagggtacc ggacggccac aggcccgctc gaaggcctgc acgacctcca
     3061 gcacgctgta gccgcgcccg gtgccaaggt tgaccgtcag cacgccgggc cgttcggtca
     3121 ggcgccgcac cgccgccacg tgaccgaccg ccagatccac cacgtggatg tagtcgcgca
     3181 cgccggtacc gtccaccgtg gcgtaatcgc cgccgaacac ccgcaggcgc ggcagacgac
     3241 ccaccgccac ctggctcaca aagggcatca ggttgttggg caggccattg ggatcttcgc
     3301 cgatgcgccc gctggcgtgg gcgcccaccg ggttgaagta gcgcagcagc gccacccgcc
     3361 aggcgggatc ggccgccgcg agatcggaca gcacgtattc catcatccac ttggtgcgtc
     3421 cgtagggatt ggtcggcccg gtcgggaaat cttcgcggat cggcacgctg gccggatcgc
     3481 cgtataccgt ggcggaagag ctgaacacca gggacttgac ccccgcgtcg ctcatcgcct
     3541 cagccagggc caccgtgccg gcgatgttgt tgtcgtagta catcagcggc ttggcgaccg
     3601 attcgcccac cgccttgagg gccgcaaaat gaatcaccgc gtcgatggca tgggtgcgga
     3661 agatctcccg cagggcggcc ttgtcacgga tgtcgctgcg gtagaaggcc gccagcggac
     3721 ggccagcgat cgcctcaacc cgcttcaggg cctcgggctt gctgttgctg aagttgtcca
     3781 ccaccaccac gtcgtagccc gccgcgagca gctccacgca ggtgtgggaa ccgatgtagc
     3841 cggcgccgcc ggtcaccagg attgtcggca tgaaaaattc cattcaagta acagaaagcg
     3901 caatgatagc ccgtcgtcct gacaggacat cgccacatat tcatcagatc gcgccattct
     3961 cccgcaaggc ggtcacggcg gccggcgtgt agcccagatc ggccaggatg gcatcggtgt
     4021 gctcacccag cgtcggcccc agccatgcgg tggtgcccgg agtcccggtc agccggggca
     4081 cgatgcccgg aaccctgagg ggcgtgccgt cggccaggtt cgcggattcg atcatgcccc
     4141 gcgccagaaa ctgggcatcg cggaacatgt cggccaccga gaagacggag ctggccggca
     4201 cctgggcggc ttccagcgcg gcgagcaccg cgtcaccgtc atgggccgtg caccaggcgt
     4261 cgatggcggc gtagagctcc gccgcccgcg cgtcgcgtcc ggcgttgctg gcgagggtcg
     4321 ggtcgcccgc caggtcaggc cgcccaatgg cctgcatcag acgcaggaag atggcatccc
     4381 cgttgccgcc gatgatcacg tggcggccgt cgcgggtggt atgggtgttg gacggggtga
     4441 tgcccggcat aatgttgccg gtccgctcgc gcacgaaacc gaacaggtcg aactcgggca
     4501 ccatgctctc catcatcgcg aagaccgcct cgtagagcgc cacgtccacc acctgcccgg
     4561 cgccgccatt cacctcccgg tgacgcaggg ccatcaaggc gccgatggcg gcccacaggg
     4621 cggcaatgga gtcgccgatg gaaatcccgg tgcgcacggg cggccggtcc ggaaagccgg
     4681 tgatgtagcg caacccgccc atggactcgc cgatcgcccc gaaaccgggc cgatccttgt
     4741 agggcccggt ctggccgaag cccgacagac gcaccatcac cagccccgga ttcgccgccg
     4801 acagcgcctc ccagcccaac ccgaggcggt ccagcacgcc cgggcggaag ttctccacga
     4861 cgatgtccgc ctccgccgcc agccggcgca cgacggccag gccatccgga tgcttgaggt
     4921 tggcggtgag ggagcgcttg ttgcgtgcct gcacggccca ccacagggag gtgccttcgt
     4981 gcagcttgcg ccactggcgc aggggatcgc cgccgtcggg cgattccacc ttgatcacgt
     5041 cggcgccgaa ttcggccagg atgcgggtac agaaggggcc ggcgatgagg gtgcccagct
     5101 ccagcacctt cacgccctga aggggtttgt ccatgggagg tcctgatcgg gcgcccggcc
     5161 gcggcacagc gctcgcccta cagggcgcgc cgctccacca gggcctgggc aatggtgccc
     5221 gtgtcggtgt gttccagttc gccgcccacc ggaatgccgc gggcgatgcg gctcaccctg
     5281 agcccgcggc tgcgcagcag ctcggagatc atgtgggccg tagcttcgcc ctcgttggtg
     5341 aaattggtgg cgaggatgac ctcctgcacc acgccgtccg tcgcccgggc aaggagcttt
     5401 tccagatgca gctcccgcgg tccgatgccg tccaggggcg acaggcgccc catcagcacg
     5461 taatacaggc cctggtagct gtgcgtctgc tccagcatcg ccagatcagc gggcatctcc
     5521 accacgcata gctgcgtccc gtcgcgccgc gggttggcgc agcgggcaca gacctcgtcc
     5581 tcgcacagcg tattgcagcg ggtgcagtgg cgcagcacct ccagcgcatg gcccagggcc
     5641 gtggccagcc gcgccgcacc gcgctgatcg cgctggagca ggtggtaagc catgcgggtt
     5701 gccgacttgg gccccacgcc gggcaggcag cgcagggctt cgatcaaggt atcgaggctg
     5761 gacgggctgc tcatggacgc ggccaacgcc tcagaacggc agcttgaagc ccggcggcag
     5821 gttgaggccg gcggtgaagc cggacatctt ttcctgggtg gtggactcga cacggcgcac
     5881 ggcgtcgttg agagccgcgg cgatcaggtc ctcgagcatc tccttgtcgt ccatcaccga
     5941 cgggtcgatg ctgacccggc gcacgtcgta cttgcaggtc atagtcacct tcaccatgcc
     6001 tgcgccggac tggccttcga cctcgatgcc gccgaggctg tcctgcatct tcttcatgtt
     6061 ttcctgcatc tgctgggcct gtttcatcag gccggcgatt ccgcctttca tcatggcgca
     6121 ttgactccgt tgaagttaaa ggggcttgac cgacgattct tccatcgtcg catcgaaacg
     6181 ttcgatcagt tcgcgaacaa agggatcctg ctccagcgcc gccaccgccg ccgcgtggcg
     6241 ctcggcccgc acggcctcgt tgcgctgggc aggggtttcg gtggcgatct cgccgacttc
     6301 cacgtgcacc cgcagaggcc ggccgaaatg ggcggccagg gcctcctgca gcttttcggg
     6361 accgctgcgg ttgatggcga gcaggtggcg gtgggtgtga ccgaggcgca gacgcagcgt
     6421 gccgtcggta gtgccggtca gctcgcagtg ctgggccagc tcgcgcacca tgccgccgag
     6481 gccgagatca gccagcaggg catgccagtc gaggccggcc ggcagcgggg ggagatcacc
     6541 ggacggcagt tctgcaggcg acgcctcgac gggccggtcg ggcgcaacgg ccgagggcag
     6601 cgcagccggt tccgccggac gcgcgggcgc ttcggccaca ggcagggcgg gcgggacctc
     6661 caggatgggt gccgctgcaa aaccatgggc ctccggcggc agatcctccc agggcggcac
     6721 gctgctgccc atttccggcg caggcgcgac cgcgggaacc tgcgtggcgt gctgcggaac
     6781 gggtaccgcc gagcgctgtg ccaccaccgg caaaggcgtc ggggcaggcg ccgtacggag
     6841 cgcggccggc gtggccgtcg acagcgccgg agagggcgca ggacgctccg caggcgcgcc
     6901 tcgactcgtc gcagccggca gcggacgcgt gtgtcccgtc ccgccaccgc cggaggcggc
     6961 cgcgctgccc agggccggcg gctgctcggg gcggaaggca aacagacgca gcaggctcat
     7021 ggagaagccg gttgcctcgt cgggcgcgag gggcagctcg tcgcggccgt ggatggcgat
     7081 ctggtaggcc agctgcagga attcggcgtc aaaggcctcg gcgtagggcg ccagccgggc
     7141 ggcctcgaat tcgtcggtca ccgcggtggg tgccagttgc accagcgcaa tgcgatggag
     7201 caggctggca agggactgca gggcggaatc gaaggacagg ctgcgtgcct ccatgccttc
     7261 ggctgcagcc atcaggccct gcacgtcctg ggccaccagg gcatccagaa tggcgtagag
     7321 gtgatcgtcg cccaccgtgc ccagcatgtc ctgcacagcc tgctcttcga ccttgccggc
     7381 gccgtgggcg atggcctggt cgagcaggga cagggcgtcg cgcatggagc cccccgccgc
     7441 cttggccagg tgacgcagcg cacccggctc gaagggcacg gcctcggctt ccagcagccg
     7501 cgtcaggtgg ccgacgatgt gccccggcgg catctgcttg aggttgaact gcaggcagcg
     7561 ggacagcacc gtgaccggga tcttctgcgg gtcggtggtg gcgatgatga acttgacgtg
     7621 ctccggcggc tcttccaggg tcttgagcat ggcgttgaag gcatgcccgg tgagcatgtg
     7681 cacttcgtca atcatgtaga ccttgtagcg gccctgcacc ggtgcataca cggcctgatc
     7741 gagcagcgcc gccatgtcgt ccacgccgcg gttggaggcg gcgtccatct ccacgtagtc
     7801 gggaaagcgg tcggcgtcga tggcgacgca ggcggcgcac tggttgcacg gcgcggcggt
     7861 gacgcccgtc tcacagttga gggccttggc gaggatacgc gagatggtgg tcttgcccac
     7921 cccgcgggtg ccggtgaaca gataggcgtg gtgcagccga ccggtggcca gggcgtgggt
     7981 cagggcacgc accacgtgct cctgcccgac gagcgtctcg aaactgcgcg ggcgccactt
     8041 gcgggcaagt acttgataag ccatggccgg attctatcag gccggcagcc tgctccggga
     8101 aaccgcgcat ccgcccctcg gacgcactca ggtgcccgcc gcgggcacgc cgctacagct
     8161 tgccgctcac ccgcatgccc tccagacgta gcgcaagggc ctgctccagc tcgctgagac
     8221 gttgccggat cgcctgtcgc cgcgcatccg aggacgccgc ctccagctcg gcccgcaggc
     8281 gcgcgcgctg gtggaagagg tcgatctccg gcggcggcgc accggcattc ttgaggatct
     8341 tgaaggccat gcggaactcg cccggggtct cggaccagcc ctccggctcc ggcatcggct
     8401 tgccgaagct ggcagcggtc tgcaactcac ccgaccgcaa ggcctcggcc aggtgcaggc
     8461 cgatggcctc gtcctggcaa cggatctcgc tatcgcggcg cttgcgccgt tcggaatcgg
     8521 aaggatcgga tgtcatggca ccccaacggc cgggcagcgc cgccccaacc cgaccggatc
     8581 agtcctgcgt cccgcagccg gcggggcgac gcacccggcg atcggatggc gggtgcgtgg
     8641 cgagcttctc ggccggacgc cgcggccgcg ccaccaattc gccgccgcaa ttggggcagc
     8701 ggccgccgag cacctcttct gcgcaggtcg cacaaaaggt gcattcgaac gagcagatgc
     8761 gggcctcggc ggagtcgggc ggaagatccc ggtcacagca ttcacagccc ggtcgaagtt
     8821 caagcatggc gacgctcccg gatgaggacg accggtgtcg tcccgttgat gaacatcccc
     8881 gtcacgacgg gggcggtgta ccagaaaaca ggaggcgagg gccggagcgt cctagcgggg
     8941 cgcgttcaac gccgccacgc tggcttccca ttcccgacca ttgaagggca ccacctcggg
     9001 ctgccgcgcg gtcacatcgt agtcgtccag tacccgcaca ttcaccgtat agagttccgg
     9061 cgccgcccgc ggccgtgtga atacgtggat gccgcacacg gtacagaaat gatggcgcgc
     9121 cacgccggaa ccgaaccggt aggtgccaag cacctcctcg ccgctcaaca ggctgaagcg
     9181 ctccggcgcc acccggtggt gcaggatgcc cttcttgcgg cagatggagc aattgcattc
     9241 ggtcacgcgg atgatctccg tgtcgattgc aaaacgtacc gcgccgcaat gacagcttcc
     9301 atgatagatc gccatgcgaa ctcctcccgg ctgaaacgcc acggcgcccg tcagcactga
     9361 tgcgcgcatt gtcgctcgcc tccacgccgc gcagcaggcg ccgccgaacc acggagaaca
     9421 gcccggcgtt gcgcagcgcg cggaccacct ccgcgccaga ccgggttgcc gcccgcaaac
     9481 gtccgtacga taccccctgc gcgggccgac cgaccaggat ccggcatgaa aaagggcgct
     9541 cctgagagcg cccttttccg cgattcgggg tggcgagcct gacccccggc acttgcaggg
     9601 aacggctgtg gctgctgcct tccggccctg accaggttca ccgcgctgca atgcggggag
     9661 acccgccacg ccgaattcta acaggttcgt gcccactgcg gacagccccc cgttcagcct
     9721 gccaagggca ggacgatgct catctccagg ccaccgccgt cccggttgct cgcctcgatc
     9781 tgccccccgt gggaggtcac cgcacggcgc gcgatcgcca gccccaggcc gaagcccccc
     9841 gccgtcaccg ccgcgcggcc gcgatggaag ggctcgaaga tggtcgccag atcactttcc
     9901 gacacgcccg gcccccgatc ggacacgcgc agacggaaac gcccgtcgat gacgcccgcc
     9961 gtgacatcga cggccgtccc caccgccgtg aacttgaccg cattacggat cacgttctcg
    10021 aaggcgcgct ggatcaactc ggcgcgcaca cgcgtcagcg cctgtccgtc ccccacgaaa
    10081 tgcaccgtct tgccgagcgt ctcggcttcg aaacgggcgt cgtctgcgat ggcagccacc
    10141 agatcgatca gatccacggc ctcctccggc gcatcccccg aaccggcatc caggcgcgcc
    10201 agggtgagca actgccccac gagctcgtcg aggcgcacgg actcccgttc gatgcggtcg
    10261 atcgtcgccg ggagcttgga tgcatcctgc cgggccagcc cgatggccgc ctgcatgcgc
    10321 gccaggggtg agcgcagctc gtgggagaca tcgtggagca ggcgacgctg ggcgccgacg
    10381 agagcctgca gctggcccgc catgcgatcg aactccaccc ccagatcggc gatctcgtcc
    10441 cgccgcgcgc ccatcagcgg gctcacccgc gtgtcgagcc ggccgtccgc gacggcgccg
    10501 aaggcccagc gcagatggcg gaccggcttg gcgagatacc acgccagcaa cgccgacacc
    10561 agcacgctgg cgagcaatcc gctggcgatc agcacgccca gccccggtcg atccggcgga
    10621 cgccgccgct cgaaaggcgg gcccggcggg cgacccggca gatcaaggcc cggcggtggg
    10681 ccgggcggca ccccctccca gccacgattg cccgcgtccg ccggcacgaa gagcaggtaa
    10741 gtccggccgt cggcgctcag cacctgccgc accggggccg caggatgatc gtgatcggcc
    10801 ctgcggcggg cactcgccag cgcctcggcg ctcacaggcc gaccgagcag gtcgtggccc
    10861 gcctcatcca ccaccaacag gcgcggcgcg cgcccttcgg ccccccactc gtcgagcagg
    10921 ctcaataccg ccggcgtgcc gccgtgggcg aggacggccg cggcggaggc caccgcccag
    10981 ccggcccgcg gtccaccagc gagggccggg tcccgctcac gctcacgctg ctccaggtcg
    11041 gcctggtgat gccaccacac ggcagcgccc acacccaccc cggccagcag caaggccagc
    11101 cagaaggcaa agaagaattt ccagaaaagg cgcccgagct tcattcgcgg atcatctggt
    11161 agcccagccc gcgcacggtc tcgatccacg agccgccatc cgcacgtgct ccgagcttgt
    11221 ggcggatgct cgacagatgc acgtcgatgc tacggtcgaa gcgggccagc ggacgcccca
    11281 gcccctgctc cgacagctcg gtcttgctca ccacgcgacc ggcgttgcgg gccagcagtt
    11341 ccagcaggtt gtattcagtg ctggtcagct ccagcgcgcg cccctgccac tcggcgcggc
    11401 gcatctgcgg ccacatgagc agcgggccga ccgccaccgt gccgttgggg ctgtcggcgg
    11461 acgacgccgg ctcgtgggtc cggcgcagga tggcccgcag acgggccacc agctcgcgcg
    11521 gcgtgcaggg cttgggcacg taatcgtcgg cccccagttc gagaccgacg atgcggtcca
    11581 cgtcgtcccc cttggccgtc aacatgagga ccgggacgtt gctctgggca cggatccggc
    11641 gcagggcctc gatacccccg atacccggca tcatcacgtc gagcacgacg atggcgtgac
    11701 gccccgacaa ggcctcggca acaccggact ccccgtcgtg caccaccgtg gcctcgtagc
    11761 cttcctgctc gatgtactcc cgcagcaggg cggacagctc cacatcgtca tccaccagca
    11821 acacccggtt catttccgcc tcctgatctg tgcagcgcga tgatacgacc ggcctgccac
    11881 cgcgatgcgc ggggtgcagg gcatttaccg ccctttacat gcctttacat gtcgcgaacg
    11941 ggccttaacg acaaatcgcg caatgatttc cactggaaac ggactaacct gacgcccatt
    12001 tccacccaga cttttcccat gagaagaatc atcgcccacc gtccccgtca cctggccctc
    12061 gccctgcttg gcagcctgat gctgagcggc cctttgcgcg ccgccaatct gcccgatctg
    12121 ggcgacgcct cggaggccac gctcacccgc caggccgaac gtaccctcgg cgaacagacc
    12181 atgcgcagcc tgcaggcatc cggtgcgctg ctggatgatc cggaggtgaa tgcctacctg
    12241 gacagcctcg gccgccgcct gctgcaggcc gaccccagtc tggccgggca gcccttcgac
    12301 ttctttgcgg tggaatcctc cgaactcaac gccttcgccc tgcccggcgg ccacgtgggc
    12361 gtgcataccg gcctgatcct cgcgacccag agcgaaagtg agctggcctc ggtgatggcc
    12421 cacgaaatcg cccacgtcac ccagcaccac atcgcccgcc agatcgccgg ccagtccggc
    12481 gcacagctcg ccagcatggc ggcgctgggc gcggccatcc tcgccgccgc caccggcaac
    12541 gggcaggtgg cgatggccgc tgtcaccggc gccaccgccg ccaacctgca aagccagatc
    12601 aactacaccc gcgaacacga aagcgaggcc gaccgcatcg gcttccagct cctcaccggc
    12661 gccggcttcg acccccgcgc catggccagc ttcttcgacc gcctgcaaaa ggccacgcgc
    12721 ctcgtggacg gcaatgtccc cagctacctg cgcacccacc ccctcaccca cgaacggatt
    12781 gccgaggccg aggaccgggc cttcaacctg agctacaagc aggtgcggga cagcgacgaa
    12841 taccacttcg tccgcgccct gctgcgcagc tacgagggtg agccccgcga ggccgtcgcc
    12901 ctgctcgaca aggcgctcgc cgagggccgc acccagaatg cccgcgccac ccgctacggg
    12961 ctcaccgccg ccctgctgcg ggcgggcgat ttcaagcgcg ccgacaagga gctcgcggtc
    13021 gtggaacggg acggccccca ccccatggtg gaagccctcg ccggccagat actgctccag
    13081 tccggccgcc tgcccgaagc ccgcaaacgc tacgaatcgg ccctgcagcg cttcccggat
    13141 cacctgcagc tggtctacga ctatccgcgc gtgctgatcc gcctgaacga cgaagctgcc
    13201 gcggcccgct tcaccgaaag ccgcctgctt gcccgcccgc aggaccccac cctgcaccgc
    13261 ctcgccgccg aagccttcgc cgccctcggc cagcagaccc gctcccacgt tcatcagggg
    13321 gaatttgaag ctgcccgcgg caacacccag gctgccatcg agcaataccg catcgcgcta
    13381 cgcagcgccg acggcagtga gcgggatctg accatcgccg acgcccgcct gcgcgaactg
    13441 caggcccagc agcgcgagtt gcgcaaggat ggccgcgacg gaggtaaaga cccttccggc
    13501 ggacgcttac acagctttac cccgcgctga ccgcgcttta ccggctgaaa aaggaagatg
    13561 tgatccgtca ggcccgccga agctccggcc cctggacgca ccttccgacg acttacagga
    13621 gtccatcatg tcctcgctct tcacgaatcg cttcaagacc ctactcgccg tggccggtat
    13681 cgcggtcgcg accccctttg ccgcccacgc cttcggcggc cacgaacact gcggcggccc
    13741 gatgatgcac cgtgaaatgg gtttcggtgg ccccgaaggc ggcgccatgc acctgccgcc
    13801 cttcgtccgc gaactcaagc tctccgatgc ccagcgcgac cagatcttca agatcatgca
    13861 cgagcaggca ccggccttcc gcgacaaggc catcgccgcc cgcaaggcgc acgaagagct
    13921 gcgcgacatg gctttctcgg ccaggtatga cgaggcccgc gtcgcggccc tcagcgacac
    13981 cgaagcccgc gccatggccg agctcaccca gatgcgtctg cgcaccggca accagatcta
    14041 ccaggtgctg acgccggaac agaagaagaa ggcagacgag ctgaaggccg acttcgagac
    14101 ccgcatgacc cgcccggtaa gggatggcgg ccagccgggc aagcgcgaac cgctccgcaa
    14161 ctgaccttgc ctctggagac ccatgctcca gcgcacgcgg cggagacgcg acggcacagg
    14221 ccggcgcgtc tccgcgttcc cttcaccact tcaagctgac cgagaaggac acagacccat
    14281 gcgcaacttc gacgagatcg accggaaaat cctcggaatc ctgcagcggg atggccgcat
    14341 caccatgacc gacctggcgc cccaggtcgg cctgtccgtc acgccctgca cggagcgcgt
    14401 ccgccgaatg gaacgggacg gcgtcatcgc cggctatcac gcccgtctca acccccgcgc
    14461 gctgggcctg aacgccctgc tcttcctgga actgcgcctc acgtctcgat cggcagaggc
    14521 cctgacccag atgagcgaaa cactcgcaga aatccccgac atcatggaat gtcactacgt
    14581 gtccggagat tttgactgcc tcgtcaaggc gcgcctgccg aatctgggcg aataccgccc
    14641 gttcatcgcc gacctggtcg aaagactgcc cgtggtcgaa tcccgcagct acgtggtgat
    14701 ggaagaaatc aaggagtcac cgctgctgaa cgcctgaccc gcaaaaattc gtcggccagc
    14761 gggcagaacg ctgcaaggat cggcgctcaa gcgcggcgcc cccgcgccgg cagcgattcc
    14821 gccaggacgc catgcgccgg actaccggca ctcacgcccc ctatcgggcg ccttccagct
    14881 cgtccgcctc gatccagcgg gcgaaacgcc ggatgccgtc ctcgaaatgg atgaccggct
    14941 cccactcgag ctctgcccgg gcccgttcga tgcacaactg gctgactggc acgtcaaacg
    15001 cccgccccgg cagatagttg cgctgcgtct gctgaccggt cacccgctcg atggtgtcga
    15061 gcacctcatt cagactgaga ccgcgtcccg agccgatgtt gaagacatgc tcacgagtcg
    15121 tcttttcgag cgcagccaac agggccgaga cgacgtcatc gatgtggatg taatcccgta
    15181 cgaccgaccc gtcgccccag atctcgatgg tctcgccacg cagggccttg cccagaaaga
    15241 ccgcgacggc cccctgactg gcatgcgtgc gctgccgctc gccaaagggg ttcgccagtc
    15301 gcaggaccgc gcattgcaga ccgtgcaatt cacggaacag gcccaggtac ttctcgatgg
    15361 ccagcttcgt gatcccatac gaacacaggg gatcggtcgg atggtcctcg cggatcggca
    15421 cgctacgggg aatcccttag accgtccccc ccgacgacac gaacacaacc ttcttcacgc
    15481 cgctcttgac ggcgtgggaa agcagccgga ctgtccccag gacgttgctc tccacgtcaa
    15541 acaccgggtc cgcattggac gacttgggca gggtcgtcga cacgagatgg aaacagaaat
    15601 cgcacccctc cagcgcctcg gtgacgtccg cctcgctgac aaggtctccc tccaccaact
    15661 cgaagcgggg gttcgacaga tgcgaatccc ccagcggtgc cacatgaggg cggtcaaaac
    15721 acctgaccgc atatcccttg tccagcaacg catccaccag atgcgaccct at
//
LOCUS       DAOQMM010000090        15409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1526_length_15409_cov_38.309887, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000090 DAOQMM010000000
VERSION     DAOQMM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_15409_cov_38.309887"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..925)
                     /locus_tag="PLH85_13295"
     CDS             complement(326..925)
                     /locus_tag="PLH85_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65586.1"
                     /translation="MAETDRDLGVAAAAIEAMASTKKLSEFKSFWESFLFRLERAWER
                     NQRRVQTVPGRDAKSWLSTNAKLRRSDPLLNYLKQARNAEVHAISSSIESELIVSVAD
                     RFGRPFSMKDMSLSIEGATFVIDLTSTDIGLEWYGDVALSEPKLQCVINRGKRYDPPT
                     RHLGNAIQNHHPVAISILGLNFYKGTYAALGALLNHKKP"
     gene            complement(1116..1631)
                     /locus_tag="PLH85_13300"
     CDS             complement(1116..1631)
                     /locus_tag="PLH85_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65587.1"
                     /translation="MIDLEMQLRRADRLDAITKSLGFALWQLQALEETTANYYVLVAL
                     ATRGMGIEAGRKLDDSVKGNTFGKTIHALRDSGKMPSKIEQRFLALLKERNWLVHKSR
                     ATNRGAVHDNQACDSLLVRLDQLAEEARLLLREIGKASEKFVVQSGVPIEEVDRLTHE
                     ILNSWHSTTNE"
     gene            complement(1778..2158)
                     /locus_tag="PLH85_13305"
     CDS             complement(1778..2158)
                     /locus_tag="PLH85_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65588.1"
                     /translation="MATSSHTRVAPTQLDTDVDTYSALRGLVGYTPRNVRFSTESAGA
                     ALAAMRAADEAAIQANNALTVARESLIAAQKDFHEIILGVKAEAKVIFGEDSDEVAAL
                     GLKRKSDRARPKRSSKPTPPTKES"
     gene            2829..4202
                     /gene="chrA"
                     /locus_tag="PLH85_13310"
     CDS             2829..4202
                     /gene="chrA"
                     /locus_tag="PLH85_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNB65589.1"
                     /translation="MTPSSSPDTAQPPAQPADAAFWPAFRFWLKLGFISFGGPAGQIA
                     LMHRELVERRRWISEGRFLHALNFCMVLPGPEAQQLATYLGWLMHRTPGGLAAGVLFV
                     LPAFFILVGLSWLYVAWGQVPWVAGLFYGIKPAVTAIVAQAAWRIGGRTLKNRALGAI
                     AAASFIAIFALGLPFPAIVAAAALIGWLGGRLAPELFRTGGGHGPAAAQAAPALIDDD
                     TPTPAHARFTPGRLLRVVLFGGLLWALPMALLDVAAGWDGPLTQMGRFFTQAALLTFG
                     GAYAVLPYVFQGAVAHYGWLSAAQMMDGLALGETTPGPLIMVVTFVGFVGGATHTVLG
                     PDTPFLGGLAAAAVVTWFTFLPSFVFIFAGGPFIETTHNDLHFTAPLTAITAAVVGVI
                     LNLALFFCAHVLWPHGITGGVLAGFDGFAALLAAGAAVALFRFKVGVIPVIGASAVLG
                     GLLSLLR"
     gene            4311..6053
                     /locus_tag="PLH85_13315"
     CDS             4311..6053
                     /locus_tag="PLH85_13315"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB65590.1"
                     /translation="MTSRRRFVQSLAALSVGALPRPLPAATDPSRQALVIGNAAYRFS
                     PLTNPTNDARAVSELFGRAGFTVSTHLDVGQRDMLAAIEQFGQVVRRSDTRLVVFYYA
                     GHGAQLDWRNYLLPVDAAVQTPEQMRQRCVDLNTLLGRIAGSSGKTFVIILDACRNNP
                     FGSAYTPEQKGLSQFDAPPGSLLAYATSPGNVASDGSGRNGLYTENLVRELSVPGARI
                     EDALKRVRLNVRLASRGAQVPWETTSLENDVILFDTGHARLSEAEQEKLLEEDIARWN
                     AIKNSRNADDWVAYLRAFPNGRFAEIAQMRLIRLLAEADGGRPANPAAAAVTPAVSAP
                     APMPAPAPTPPTTPTDTEAAPAIVLGAGLPVPVLIAPSGNPFSSGRYPLARHFALGDS
                     ATFRKSDLLTGLEERTWTVRVTRVDRDADRVELNRGHWVVDLMGNTLHTDNVNFDAPV
                     AWTPSEFYVGKKWTLAYRRNENGTVSTGTYAVQIVARETITVPAGTFDTFRIEGEGWS
                     ATFGSRLQSKFWLVPSLNFLVRSEVMNRNRRGQFTVTERRELVALRQGGIDTSCAAPG
                     PSGTRTLVIRNNCG"
     gene            6087..>6372
                     /locus_tag="PLH85_13320"
     CDS             6087..>6372
                     /locus_tag="PLH85_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65591.1"
                     /translation="MPRLPSAPAALRAVACLALATVLLAPVCRAADAPAADSGFARPP
                     SPHVHALILTIGAYRNGIPPLTGVARDADNAREIARRMGVPPAHITVLRDA"
     assembly_gap    6373..6472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6534..7994
                     /locus_tag="PLH85_13325"
     CDS             6534..7994
                     /locus_tag="PLH85_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB65592.1"
                     /translation="MHALILTIGAYRNGIPPLTGVARDADNAREIARRMGVPPAHITV
                     LRDAELTLDGLRRAFDQLDGRVRDGDEVFVYYSGHGGRQKVVEAGVERCAESLITVDG
                     YGFTDAELETRLKALSTRAGKMVVFLDACHSGGVTTRATRVDPPFLPKYWQGKAGGGD
                     ACARPVNVLTRDIVLATATPGSGGANYAYIAAARDNEISLDQPGKGGLATQAWLACLA
                     GQARDTDGSGGLSAEEIRVCAQQHIDAKLKNAEGFLPHHVAITGNRDMVLAYDTKAGA
                     AALPAVLPSPSATASPTPAAPTAPVAVPPAVPTALPLKASPLATLHDLYANRDDRRLV
                     QLATATPRVRIGRDEVRFTLSSREGGYVYLLMVGSNGEAFDLLFPNALDRDNRLRPGE
                     TLTLPRRSWQIGAGGPAGTDTLLALVTDSPRDFRGLGLKPVGPFSAIEPTRARDIQLV
                     TAHATPPADCPRDLAAQPACSASYGAALLSIEEVAP"
     gene            8033..8872
                     /locus_tag="PLH85_13330"
     CDS             8033..8872
                     /locus_tag="PLH85_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNB65593.1"
                     /translation="MPRPHRLTATALILALACAGFVPVRANPPPVPTASAGSEDEAMQ
                     PKFVWGILIKIAYNVASSVFSDWAKSKLSIDLSPGGLAKLFFNSSTARIVPVSLPSLG
                     FLAKQADGVGNAVAGEPTAPLVVNAQGINYQGVHVALLGFDRAGKALGYRPVTDGFRS
                     GERFKLRILPTFDGLLALDNINPQGRRAQVYPPERGTMVEVKAGVEILVPLDRDQYLE
                     FAGSTGEEKLVVTLRSPRAVGDAASPATVYRHDEAIGSSFVQETRPGTYPVIVQALSL
                     RHD"
     gene            complement(8893..9399)
                     /locus_tag="PLH85_13335"
     CDS             complement(8893..9399)
                     /locus_tag="PLH85_13335"
                     /inference="COORDINATES: protein motif:HMM:NF040052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HNB65594.1"
                     /translation="MSSDDASRRGLASPPCYAAEFPGYFGDASLAPEVVARLNALLEA
                     ERAGAKVLRLLARELPDDASARALLESVCDDEGDNAVALYRTIRRLGGEASAVTGAFV
                     EKTLAIEGLEARLRFVNTGQQWVVRKIDELLPLVHDGAARAMLDAMRQSHRDNIAACD
                     AFLATVHR"
     gene            complement(9407..10018)
                     /locus_tag="PLH85_13340"
     CDS             complement(9407..10018)
                     /locus_tag="PLH85_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65595.1"
                     /translation="MQYPDFFDAVPRLRIRDPLAEFLGAADEGVLEYRYLDAVRLAGH
                     SCPTVAGAFWLTRLALGALYCTRMAVRGGVRVAFRERVDNGVTGVVANVVSMLTGAAA
                     DGGFKGLGGQFERRGLLGFGGEQPFELRYTRADTGISVDASVQLGQVPADPDMAPLMR
                     RCLAGEADADEARRFRELWQDRVRRLLFEHGDDPDVFVIRYVN"
     gene            complement(10157..11317)
                     /locus_tag="PLH85_13345"
     CDS             complement(10157..11317)
                     /locus_tag="PLH85_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNB65596.1"
                     /translation="MKTAPHPLWLVGFRPFFVLACAAGAALPMLWALVFADTLTLPAG
                     RLSPLQWHAHEMLFGFGWAVLGGFLLTASKNWVQIRGYHGAALKALVAAWCVERIGIS
                     FGGTWPAALFWASAFAFLGAIVAMLVATLVRHRARDSFRDNLFFIVALPLFLVAKALV
                     LDADHFHAGVSMTLGLYRLAFLVMLERTLTTFMKNGCGVDLPRDPWLDGPIKGLALVL
                     VGLPWLPAGLAVALLLVEAVLLTARFFRWRPDRAFRRLELAVMYVGYLALIAQLLAEA
                     WSRIGAPGWVGSVPVHLLTVGAMGPIIPAMLVRISKGHTGRKVSFDAFDKAVLWVMLA
                     ALGLRIGLPQLAPGLYGLWVDLAAAAWGLGFGALGLRILPRVLAPRVDGKVH"
     gene            11617..13704
                     /locus_tag="PLH85_13350"
     CDS             11617..13704
                     /locus_tag="PLH85_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB65597.1"
                     /translation="MQHRFAPTTLAAALAALGLPAAHAAGDAPSVTLNPVVVTGSRAE
                     AASFDLPAAIDVLNREQLTAGQPRVNASEALIAVPGVVANNRQNYAQDLQISSRGFGA
                     RSAFGVRGIKLVADGIPASMPDGQGQAATFNLDMAERMEVLRGPFSALYGNHSGGVIQ
                     YFTREPEAAAVSGGFAFGSYGTTKADLTVEGKEGATGAIANVSRLDTNGYRDHSAALR
                     EQAFAKIGTRLSDVSTLSFVVNGLWQHDTQDPLGLTWAAAKNAPRSVDASALTYNTRK
                     SIDHLQGGVTYDRRFGSDALQLTAYSGQRSVVQYQAIPISSNAKSSGGVVDFDRNFYG
                     FGARWIARGEALGGRVTTTTGVDYDASTDKRSGYDNNKGTASTLRRRESDSVAASAVY
                     VQSEWARDAWRLTGGLRYNTVKFDVYDEYLSNGNDSGSRDYSRATPVVGVVYALNPAV
                     NLYASAAKGFETPTLNELFYSAGGTGFNFGLAAARSQHLEVGAKALVGDASRVNVALF
                     QVSTDDELVVADSTGGRTSYKNAGKTLRQGIEVSAETAWQRDLRSRVSLSHLRAVYDE
                     AFNTVFQSKAITVPAGNAIPGVPRTTLFGDLAWTPVAGITAAVEGVYRSKVYVEDSNT
                     ATPASSYALANVRLTAEQRSGHWRFSEFARVDNLFDRQYIGSVIVGDSNSRYYEPSPG
                     RNGMVGVNLRYLW"
     gene            complement(13792..14415)
                     /locus_tag="PLH85_13355"
     CDS             complement(13792..14415)
                     /locus_tag="PLH85_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB65598.1"
                     /translation="MRPDDPRHGWNSRYADAGDDYVFGTAPNAFLAAQARWLTPGQRA
                     LSVADGEGRNGVWLAEQGLQVDAVEFAPAAIAKARRLAAARGVTPNFIEADLLTWDWP
                     VSAYDVVAAIFIQFVGPAERARLFACMTAALRPGGLLLIQGYTPRQLDYRTGGPSAVE
                     NLYTQALLRDAFAGLEILHLAEYDSHVDEGHGHRGTSALIDLIARKP"
     gene            complement(14420..>15409)
                     /gene="thrC"
                     /locus_tag="PLH85_13360"
     CDS             complement(14420..>15409)
                     /gene="thrC"
                     /locus_tag="PLH85_13360"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNB65599.1"
                     /translation="GDTGSAAEYAMRGKHGVRVFMLSPHGRMSAFQRAQMYSLQDDNI
                     VNIAVRGMFDDAQDIVKAVSNDHAFKAQYKIGAVNSINWARVAAQIVYYFKGYFAATA
                     SNDQQVAFVVPSGNFGNICAGHIARQMGLPVAQLVLATNENDVLDEFFRTGVYRPRAS
                     SETHATSSPSMDISKASNFERFVFDLVGRDGAKVRELWAAVDAGGAFDLSATPAFARI
                     GDYGFVSGRSSHADRLATIRLADARYATVIDTHTADGLKVALERRDPAVPTLVLETAQ
                     PAKFEDTIVEALGRKPARPAGLENIEALPQRVQVMDVDAAAIKALIAAAVDAA"
BASE COUNT         2481 a         5506 c         4917 g         2405 t
ORIGIN      
        1 ctaccacgac cccgggcgct gcgcgccggt gatgctggtg aggaagaagg cggggcggta
       61 atcaccccgg ccaattaatc tcattttcta gacagttcat gcctgtcgag agcgctgtca
      121 aggtggcgaa gatcgatgat tggtgccaag ccggcgtaga agaggcagga gaacgcaccg
      181 ggatcaaggc ttcggaattc tccaagaacc aaggaaaatt aagtggaata tgggagttca
      241 ggcgacggaa gaatgttgcg gcaaacatga tacggcggga aagacgacga attcaggaac
      301 ggagattatt gcctagtctg tttggctacg gctttttatg atttagaagc gccccgagcg
      361 cagcataggt tcctttgtaa aaattgagcc caagaattga aatcgcaacc ggatgatgat
      421 tctgtattgc attaccaaga tgccgagtcg gtgggtcata ccgctttcca cgatttatga
      481 cgcactgaag ctttggctct gaaagcgcga catcaccata ccactctaag cctatgtcag
      541 tgctggtcag gtcgatgaca aaagttgcac cctcaatcga tagactcatg tctttcatgc
      601 taaatggccg cccaaatcga tctgcaaccg aaactatcaa ttcggactca atgctagatg
      661 aaattgcatg aacctcagcg tttctggcct gtttgagata atttaacaag ggatcgcttc
      721 ggcgcagctt ggcatttgtg gatagccatg acttagcgtc gcgacccggc acggtctgca
      781 cgcgccgttg gttgcgttcc catgcacgct caaggcgaaa gagaaacgac tcccagaatg
      841 atttgaattc actgagtttt tttgtacttg ccattgcctc gatcgctgcg gcagcaaccc
      901 caaggtctcg atctgtttca gccatttgag atttattgaa aactcaaaat ttaaaccgcc
      961 tcaagaattt ggcaatagtt tttgaatacg gggcttattg accgctcgtg gggactgagt
     1021 ccccgcaagc cgctgtaact caatctcgcc gcaaagacaa ttagatcgtt ttcgcgcagt
     1081 gaggcctagg gatttctgga acctccattt cgcgcttact cattcgtagt ggagtgccat
     1141 gaattcagaa tttcgtgcgt tagacgatca acctcttcta ttggcacacc actctggaca
     1201 acaaattttt cagaagcctt gccaatctct cgaagcaaca gtctcgcttc ttctgcaagt
     1261 tgatccaatc ggacgagaag tgaatcgcaa gcttgattgt cgtgcacggc accgcgattg
     1321 gttgcacgag atttgtgcac taaccaattg cgctccttaa gcaaggctag aaaacgctgt
     1381 tctatcttag agggcatttt tccagaatca cgtaatgcgt gaattgtttt tccgaaagta
     1441 ttgcccttta ctgagtcatc aagcttcctt cctgcctcaa tgcccatacc acgtgtagct
     1501 agtgctacca gaacgtagta gttagcagtt gtttcttcaa gtgcttgaag ttgccacaag
     1561 gcaaagccta gactcttagt gatggcatct agacgatcag cccttcgcaa ctgcatttcg
     1621 agatcgatca ttagctgacg ccggtagttg tgctgaagaa caggacgggt gtgatgcacg
     1681 agagcggcgc gcgtatccag aagtcacata ttcattattc ttacacaacg gcggcagatt
     1741 tgccgccgtt gcctcgatgc agggctaatg ccccccatta actctccttg gtcgggggcg
     1801 tgggcttgct gctgcgcttg gggcgggccc ggtcggactt gcgcttgagg cccagcgcgg
     1861 ccacttcgtc cgagtcttcg ccaaagatca ccttggcctc ggccttcacg cccaggatga
     1921 tctcgtggaa gtccttctgg gcggcgatga ggctttcgcg ggccacggtg agggcgttgt
     1981 tggcctggat ggcggcttcg tcggcggcgc gcatggcggc cagggcggcg ccggcggatt
     2041 cggtgctgaa gcggacgttg cgcggcgtgt agccgacgag tccccggagg gcggagtagg
     2101 tgtccacgtc ggtgtcgagc tgggtgggtg cgacgcgggt gtgggaagag gtggccatgc
     2161 tgtctccttt cggtgggatg atgaatgaat gtgctgcgag gctgagggta cgtcggcgca
     2221 tccttttggg atatgaacgg attcactcgt ttggggtgaa tttggtcaca tccgggccgg
     2281 tccggcaggc catgcggctg ttggggcggc tctccacggc cggtgagggc ctcggatcgc
     2341 ggcgcgggtg gggcgtcgtc gcaggggggt gaagggcgtg ccgggccgcg ggcatcccgg
     2401 cgggggctcg gtgtggcgcg cgccggggcc ctcgggcggc ctgggggtgg gattgtccgc
     2461 gcacccttgc gtgaaggatt cacagggccg cgaatccttc acgcaggcgt gtgaatccta
     2521 cacgcagcct tgcgaatcct tcacgcaggg ctgtgaatcc ttctcgcagc cttgcgaatc
     2581 cttcacacac gtctgtgaat ccttcccaca gccttgcgaa tccttcacgc aggcgtgcga
     2641 atccttctca cgggggtgtg aatccttcgc gcagcgggga ggcctggggt cgtccggagg
     2701 ctgcatcggc ggccccacct tcgcccgccg cggcgcacca acatgctcta cagtgcggcg
     2761 gtgaggcacg ggtgccggcc gagtgccgtt ccctgccccc tcgcccccgt cttccggagc
     2821 ccgcccccat gacgccctcc tcttcgccgg acacggcgca gccgcctgcg cagcctgccg
     2881 acgccgcctt ctggccggcc ttccgtttct ggctcaagct gggcttcatc agctttgggg
     2941 ggccggcggg gcagattgcg ctgatgcatc gggaactcgt cgaacggcgg cgctggatct
     3001 cggaggggcg ctttctgcac gccctcaact tctgcatggt gctgcccggc ccggaggccc
     3061 agcagctggc cacctacctg ggctggctga tgcatcgcac gccggggggc ctcgccgcgg
     3121 gggtgctctt cgtgctgccg gctttcttca tcctggtggg actgtcctgg ctctacgtgg
     3181 cctggggaca ggtgccctgg gtggcggggc tgttctacgg catcaagccg gcggtcaccg
     3241 ccatcgtggc ccaggcggcc tggcgcatcg gcgggcggac gctgaagaac cgggcgctgg
     3301 gcgccatcgc ggcggcgtcc ttcatcgcca tctttgcgct ggggctgccc tttccggcca
     3361 tcgtcgccgc ggcggcgctg atcggctggc tgggtggacg tctcgcgccg gagctgttcc
     3421 gcaccggggg cgggcatggt ccggccgccg cccaggccgc gcctgccctc atcgacgacg
     3481 acacgcccac gccggcgcac gcccgcttca cgccggggcg gctgctccgc gtggtgctgt
     3541 tcgggggcct cctgtgggcg ctgccgatgg ccctgctgga cgtcgccgcg ggctgggatg
     3601 gccccctcac ccagatgggc cgcttcttca cccaggcggc gctgctcacc ttcggcggcg
     3661 cctacgcggt gctgccctat gtgttccagg gcgcggtggc ccactacggc tggctcagcg
     3721 ccgcgcagat gatggacggc ctggcgctgg gcgagaccac gcccggcccg ctgatcatgg
     3781 tggtgacctt cgtcggcttc gtgggcggcg ccacccacac cgtgctgggg ccggacaccc
     3841 ccttcctggg cgggctcgcg gcggccgcgg tggtgacctg gttcaccttc ctgccgtcct
     3901 tcgtcttcat ctttgccggc ggccccttca tcgagaccac ccacaacgac ctgcacttca
     3961 ccgcgcccct caccgccatc accgccgccg tggtgggggt gatcctcaac ctggccctgt
     4021 tcttctgcgc ccacgtgctg tggccccacg ggatcacggg cggcgtgctg gccggcttcg
     4081 acggcttcgc ggcgctgctc gcggcgggcg cggccgtcgc cctgttccgc ttcaaggtgg
     4141 gggtgatccc ggtgatcggc gccagcgcgg tcctgggcgg gctgctgtcg ctgctgcgct
     4201 gaacgcccgg acgcgacgcc caccacgcac cgccgcgccc gccctcttga tccgtcggcc
     4261 gcagccgtta tgctcggggt ccgagtccgt ccgccgtgtt tgcctgcctc atgacttccc
     4321 gtcgccgctt cgtccaaagc cttgccgccc tgtccgtggg cgccctgccc cgtccgctgc
     4381 cggcagcgac cgacccgagc cgtcaggccc tggtgatcgg caacgccgcc taccgcttct
     4441 cgcccctcac caaccccacg aacgacgccc gcgccgtgag cgagctgttc ggccgcgccg
     4501 gttttacggt gagcacccac ctggacgtgg gccagcgcga catgctggcg gccatcgagc
     4561 agttcggcca ggtggtccgc cgcagcgaca cccggctggt ggtcttctac tatgccggcc
     4621 acggcgccca gctcgactgg cgcaactacc tgctgccggt ggatgccgcg gtgcagacgc
     4681 cggagcagat gcgccagcgc tgcgtggacc tgaacaccct gctcgggcgc atcgccggca
     4741 gcagcggcaa gaccttcgtg atcatcctcg acgcctgccg caacaacccc ttcggcagcg
     4801 cctacacgcc cgagcagaag ggtctctccc agttcgacgc gccgcccggc agcctgctcg
     4861 cctatgccac ctcgcccggc aacgtggcct cggacggcag cgggcgcaac ggcctgtaca
     4921 ccgaaaacct ggtgcgcgaa ctctccgtgc ccggcgcccg catcgaggac gccctcaagc
     4981 gggtgcgcct caacgtgcgc ctggcctccc gcggcgccca ggtgccctgg gagaccacct
     5041 ccctcgaaaa cgacgtgatc ctcttcgaca ccggccacgc caggctcagc gaggccgagc
     5101 aggagaagct gctggaagag gacatcgccc gctggaacgc catcaagaat tcccgcaacg
     5161 cggacgactg ggtggcttac ctgcgcgcct tccccaacgg gcgcttcgcc gagatcgccc
     5221 agatgcgcct gatccgcctg ctggccgaag cggacggcgg gcgcccggcg aatccagccg
     5281 cggcggcggt cacgccggcg gtgtccgcgc ctgcgccgat gcctgcccca gcgcccacgc
     5341 ccccgacgac gcccaccgac accgaagccg cccccgccat cgtcctcggt gccggcctgc
     5401 cggtgccggt gctgatcgcc ccctccggca accccttctc gtccggccgc tacccgctgg
     5461 cccgccactt cgccctgggc gattccgcca ccttccgcaa gtccgacctc ctcaccggcc
     5521 tggaggaacg cacctggacc gtgcgcgtga ccagagtcga tcgggatgca gaccgggtcg
     5581 agctgaaccg gggccattgg gtcgtggacc tgatgggcaa caccctgcac accgacaacg
     5641 tgaatttcga tgcgccggta gcgtggacgc cctcggagtt ctacgtgggc aagaagtgga
     5701 ccctcgccta ccgccgcaac gagaacggca ccgtgtccac cggcacctac gccgtgcaga
     5761 tcgtggcgcg ggagaccatc accgtgccgg ccggcacctt cgacaccttc cgcatcgagg
     5821 gcgaaggctg gagcgccacc ttcggcagcc gcctgcaatc caagttctgg ctggtgccca
     5881 gcctcaactt cctggtccgc tccgaggtaa tgaaccgcaa ccgccgcggc cagttcaccg
     5941 tgaccgagcg gcgcgaactg gtggccctgc gccagggtgg catcgacacc agctgcgccg
     6001 cccccggccc gagcggcacc cgcaccctgg tgatccgtaa caactgcggc tgacgccgcg
     6061 cgcccaccga caccgtgatc cccgccatgc cccgcctccc ttccgccccg gccgccctac
     6121 gcgccgtggc ctgcctcgct ctggccaccg tgttgttggc gcccgtctgc cgcgccgccg
     6181 acgctcccgc cgccgacagc ggcttcgccc gcccgccttc gccccacgtg catgcgctga
     6241 tcctcaccat cggcgcctac cgcaacggca tcccgcccct caccggggtg gcgcgggacg
     6301 ccgacaacgc ccgcgaaatc gcccgccgca tgggcgtgcc gccggcccac atcaccgtgc
     6361 tgcgggacgc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgggcgt
     6481 gccgccggcc cacatcaccg tgctgcggga cgccgagctg accctcgccc cgcgtgcatg
     6541 cgctgatcct caccatcggc gcctaccgca atggcatccc gcccctcacc ggggtggcgc
     6601 gggacgccga caacgcccgc gagatcgccc gccgcatggg cgtgccgccg gcccacatca
     6661 ccgtgctgcg ggacgccgag ctgaccctcg acggcctgcg ccgcgccttc gaccagctcg
     6721 acgggcgggt cagggacggc gacgaggtct tcgtctatta ctccggccac ggcggtcgcc
     6781 agaaggtcgt ggaggccggc gtcgagcgct gcgccgaatc cctcatcacc gtggacggct
     6841 acggcttcac cgacgcggag ctggaaaccc gcctcaaggc cctgtcgacc cgcgccggca
     6901 agatggtggt cttcctcgac gcctgccatt cgggcggcgt gacgacccgc gccacgcgcg
     6961 tcgatccgcc cttcctgccc aaatactggc agggcaaggc cggcggcggc gacgcctgcg
     7021 cgcgaccggt caacgtgctc acccgggaca tcgtgctggc caccgccacg ccgggcagcg
     7081 gcggcgccaa ctacgcctac atcgccgccg cccgcgacaa cgagatctcc ctcgaccagc
     7141 ccggcaaagg cggcctcgcc acccaggcct ggctcgcctg cctggccggt caggcgcggg
     7201 acacggacgg cagcggcggg ctctccgccg aagagatccg cgtctgcgcc cagcaacaca
     7261 tcgacgcgaa actgaagaac gccgagggct tcctgcccca ccacgtggcc atcaccggca
     7321 accgggacat ggtgctggcc tacgacacga aggcgggcgc cgcggcgctc cccgccgtgc
     7381 tgccaagccc ctccgccacc gccagcccca ccccggcagc gcccacggcg ccggtcgctg
     7441 tgccgcccgc tgtgccaacc gccctccccc tcaaggcttc gcccctggcc acccttcacg
     7501 acctgtacgc caaccgggat gatcgtcgcc tggtgcagct cgccaccgcc acgccgcggg
     7561 tgcgcatcgg ccgcgacgag gtgcgcttca ccctgagctc gcgggaaggc ggttacgtct
     7621 acctgctgat ggtcggctcc aacggcgagg ccttcgacct gctcttcccc aacgcgctgg
     7681 accgggacaa ccgcctgcgg cccggcgaga ccctgacgct gccacggcgc agctggcaga
     7741 tcggcgccgg cgggccggcg ggcacggaca ccctgctcgc cctggtgacg gacagcccac
     7801 gggatttccg cggcctcggc ctcaagccgg tcggcccctt ctcggccatc gagccgaccc
     7861 gcgcgcggga catccagctc gtcaccgccc acgccacgcc ccctgccgac tgcccacggg
     7921 atctggccgc ccagccggcc tgctccgcgt cctacggcgc ggccctgctc agcatcgagg
     7981 aagtggcgcc ctgaagcgcc caccctcgcc cacctcccat cggaacgcct tcatgccgcg
     8041 ccctcaccgc ctcaccgcca ccgccctgat cctcgccctg gcctgcgccg gcttcgtgcc
     8101 ggtccgcgcc aatccaccgc ccgtgcccac cgcgtctgcc ggcagcgagg acgaggcgat
     8161 gcagcccaag ttcgtatggg gcattctcat caagatcgcc tacaacgtgg ccagctcggt
     8221 cttttccgac tgggccaaga gcaagctgag catcgacctc tcccccggcg ggctggccaa
     8281 gctcttcttc aacagcagca ccgcgcgcat cgtgccggtg tccctgccgt ccctcggctt
     8341 cctcgccaaa caggcggacg gggtcggcaa cgcagtggcc ggcgagccga cagcgccgct
     8401 ggtggtgaac gcccagggca tcaactacca gggcgtgcat gtggccctgc tgggcttcga
     8461 ccgggccggc aaggcgctgg gctaccggcc ggtgacagat ggcttccgct cgggcgagcg
     8521 cttcaagctg cgcatcctgc ccaccttcga cggcctgctg gccctggaca acatcaatcc
     8581 ccagggccgc cgcgcccagg tctatccgcc ggaacgaggc accatggtgg aggtgaaggc
     8641 cggcgtggag atcctcgtcc cgctggatcg cgaccagtac ctggaatttg ccggcagcac
     8701 cggcgaggaa aagctggtgg tcaccctgcg ctcaccccgc gcggtgggcg acgcggcctc
     8761 cccggccacc gtgtatcgcc acgacgaggc catcggttcc agcttcgtgc aggagacgcg
     8821 gcccggcacc tacccggtga tcgtgcaggc cctgagcctg cgccacgact gagcgccgcc
     8881 ggggacgccg gatcagcggt ggacggtggc cagaaaggcg tcgcaggcgg cgatgttgtc
     8941 ccgatgggac tggcgcatgg cgtccagcat cgcccgggct gcgccgtcgt gcaccagggg
     9001 cagcagctcg tcgatcttgc gcacgaccca ctgctggccg gtgttcacaa agcgcagacg
     9061 cgcctccagg ccttcgatgg cgagggtctt ctcgacgaag gcacccgtca ccgccgaggc
     9121 ctcgcccccc aggcggcgga tcgtgcggta cagggccacc gcgttgtcgc cctcgtcgtc
     9181 gcagacgctt tcgaggagcg cgcgcgccga cgcgtcgtcc ggaagctcgc gcgccagcag
     9241 gcgcagcacc ttggcgccag cgcgctccgc ttcgagcagg gcgttgaggc gggcgacgac
     9301 ttcgggcgcc agggacgcgt caccgaaata gccgggaaac tccgccgcat aacagggcgg
     9361 cgaggccagg ccgcggcggg aggcgtcgtc gctgctcatg gccgcctcaa ttcacgtagc
     9421 ggatcacgaa cacgtccgga tcgtcgccgt gctcgaacag caggcggcgc acccggtcct
     9481 gccacagctc gcggaagcgc cgcgcctcgt ccgcgtcggc ctcccccgcg aggcagcggc
     9541 gcatcagcgg cgccatgtcc ggatcggccg gcacctgccc gagctgcacc gacgcatcca
     9601 ccgagatgcc cgtgtccgcg cgggtgtagc gcaactcgaa gggctgctcg ccgccgaagc
     9661 cgagcaggcc gcgacgctcg aactggccgc ccaggccctt gaagcctccg tccgccgccg
     9721 cgccggtgag catggacacc acattggcca ccacgccggt gacaccgttg tccacgcgct
     9781 cgcgaaacgc cacccgcacc ccgccccgca ccgccatgcg ggtgcaatac agggcgccga
     9841 gggccagacg ggtgagccag aaggcgccgg caacggtcgg gcaggaatgt ccggccagac
     9901 gcaccgcgtc gaggtatcga tattcgagga cgccctcgtc cgctgcgccg agaaattccg
     9961 ccagcgggtc ccgtatccgc aggcgcggca cggcatcgaa aaaatccggg tactgcatac
    10021 acgctccctc actgccgggc ggctgaatgt tctggtctca ccgccccaag cccagaaggg
    10081 tattcgatcc ttccatagaa aaccttgatc aatctcacgt ctggcacatt tttcctacgg
    10141 gtaaaccctg agcccctcaa tgcaccttgc catccacccg gggcgccagc acccggggca
    10201 ggatgcgcag ccccagcgcg ccgaagccga gtccccaggc cgccgccgcc agatcgaccc
    10261 acagcccgta gagccccggc gcgagctgcg gcaggccgat gcgcagcccc agcgccgcca
    10321 gcatcaccca cagcacggcc ttgtcgaagg catcgaagct caccttgcgc cccgtgtgac
    10381 ccttggagat ccgcaccagc atggccggaa tgatcgggcc catcgccccc accgtgagca
    10441 gatgcaccgg cacgctgccc acccagcccg gcgcaccgat gcggctccac gcctcggcca
    10501 gcagctgggc gatcagggcc agatagccca cgtacatcac cgccagctcc aggcggcgga
    10561 aggcccggtc cgggcgccag cggaagaagc gggccgtgag caggaccgcc tcgacgagca
    10621 gcagcgccac cgccagcccc gcgggcagcc agggcaggcc gacgaggacc agcgccaggc
    10681 ccttgatcgg cccgtccagc cacggatccc gcggcaggtc gacgccgcag ccgttcttca
    10741 tgaaggtcgt cagggtgcgc tccagcatca ccagaaaggc cagccggtac aggcccaggg
    10801 tcatgctcac gcccgcgtgg aagtggtccg cgtccagcac cagcgccttg gcgacgagga
    10861 acaggggcaa cgcgacgatg aagaacaggt tgtcacggaa gctgtcccgc gcccggtggc
    10921 gtaccagggt ggccaccagc attgccacga tggcgccgag gaaggcgaag gcgctggccc
    10981 agaacagggc cgccggccac gttccgccaa agctgatgcc gatccgctcc acgcaccacg
    11041 ccgccaccag ggccttcagg gccgcgccgt gatagccgcg gatctgcacc cagttcttcg
    11101 aggcggtcag cagaaagccc ccgagcaccg cccagccgaa gccgaagagc atctcgtggg
    11161 catgccactg cagcggtgac aggcggccgg ccggcagcgt cagcgtgtcg gcaaatacca
    11221 gcgcccacaa catgggcagc gccgcaccgg cggcgcaggc aaggacgaag aagggacgga
    11281 atcccaccag ccagagggga tgcggggcag tcttcatggg tgcggcgcaa tgcggaaaag
    11341 ggccagcata gcggcagcgg ccggcgcgcg ccttgatcga gggcaggccg aggcggcacc
    11401 cgtccaggcc accgcgcgcc acccaccgtt ccggccgtcc ccaacacgga cgcagcccca
    11461 tcgacccgcg acgccttccc cgccccatgc agaaaacctc cggctgacgc ccatctcatg
    11521 attttcgtga ggcgcgcggc gtggaatcag gctatcgttg cgccgctccc ggccacctcc
    11581 cggggatcag aacagccaga aacacggaga cgccccatgc agcaccgctt tgccccgacg
    11641 accctcgccg ccgccctcgc cgctctgggc ctgcccgccg cccacgcggc cggcgacgcc
    11701 ccgtccgtca ccctcaatcc ggtggtcgtc accggcagcc gcgccgaagc cgccagcttc
    11761 gacctgcccg ccgccatcga cgtcctcaac cgggagcagc tcaccgccgg ccagccccgg
    11821 gtcaacgcgt ccgaagccct catcgcggtc cccggtgtgg tcgccaacaa ccgccagaac
    11881 tacgcccagg acctgcagat ctcgtcccgc ggcttcggcg cccgctctgc cttcggagtg
    11941 cgcggcatca agctggtggc cgacggcatc cccgccagca tgcccgacgg ccagggtcag
    12001 gccgccacct tcaacctgga catggccgag cgcatggaag tgctgcgcgg gcccttctcg
    12061 gccctctacg gcaaccactc cggcggcgtg atccagtact tcacccgcga accggaagcc
    12121 gcggcggtct ccggcggctt cgccttcggc agctacggca ccaccaaggc cgacctcacc
    12181 gtggaaggca aggaaggcgc caccggcgcc atcgccaacg tctcgcgtct cgataccaac
    12241 ggctatcgcg accacagcgc ggccctgcgc gagcaggcct tcgccaagat cggcacccgc
    12301 ctgagcgacg tgagcaccct cagcttcgtg gtgaatggcc tgtggcagca cgacacccag
    12361 gatcccctgg gcctcacctg ggccgccgcc aagaatgccc cgcgcagcgt ggacgcctcc
    12421 gctctcacct acaacacccg caagagcatc gaccacctgc agggtggcgt cacctacgac
    12481 cgccgctttg gcagcgatgc cctgcagctc accgcctaca gcggccagcg cagcgtggtg
    12541 cagtaccagg ccattcccat ctccagcaac gccaagagct cgggcggcgt ggtggatttc
    12601 gaccgcaact tctacggctt cggcgcccgc tggatcgccc gcggcgaggc cctgggcggc
    12661 cgcgtgacca ccaccaccgg cgtggattac gacgccagca ccgacaagcg cagcggctac
    12721 gacaacaaca agggcaccgc ctccaccctg cgtcgccgcg agagcgactc agtggccgcc
    12781 tccgccgtgt acgtgcagag cgaatgggcg cgggacgcct ggcgcctgac cggcggcctg
    12841 cgctacaaca ccgtcaagtt cgacgtctac gacgaatacc tgtctaacgg caacgacagc
    12901 ggcagccgcg actacagccg cgccaccccg gtggtgggcg tggtgtatgc gctgaatccg
    12961 gcggtcaacc tctacgccag cgccgccaag ggcttcgaga cgcccaccct caacgaactg
    13021 ttctactcgg ccggcggcac gggcttcaac ttcggcctgg ccgccgcccg cagccagcac
    13081 ctggaagtgg gcgccaaggc cctggtgggc gacgccagcc gcgtcaatgt ggccctgttc
    13141 caggtgagca ccgacgacga gttggtggtg gccgactcca ccggcggtcg caccagctac
    13201 aagaacgccg gcaagaccct gcgccagggc atcgaggtat ccgccgagac cgcatggcag
    13261 cgcgacctgc gcagccgcgt gtccctgtcc cacctgcgcg cggtgtacga cgaggccttc
    13321 aacacggtct tccagagcaa ggccatcacg gtgccggccg gtaacgccat ccccggcgtg
    13381 ccgcgcacca ccctcttcgg cgatctggcg tggacgccgg tggcgggcat caccgcggcg
    13441 gtggaagggg tttatcgcag caaggtctac gtggaggatt ccaacaccgc cacgccggcc
    13501 tcgtcctacg ccctggccaa cgtccgcctc accgccgagc agcgcagcgg ccactggcgc
    13561 ttcagcgaat ttgcgcgggt ggacaacctc ttcgaccgcc agtacatcgg ctcggtcatc
    13621 gtcggtgaca gcaacagccg ctattacgaa ccctcccccg gccgcaacgg catggtgggg
    13681 gtgaatctgc gctacctgtg gtgagtgtgg cgggcgccgg gcctgtgacg gcgcgacccg
    13741 acaacaaaaa accggcctgc gtggccggtt ttttgttgga cgtcccgccc ctcagggctt
    13801 gcgggcgatc aggtcgatga gggcggaggt gccacggtgg ccgtggcctt cgtccacatg
    13861 gctgtcgtat tccgccaagt gcaggatctc cagcccggcg aaggcgtccc gcagcagggc
    13921 ctgggtgtag aggttttcca ccgccgacgg gccgccggtg cggtagtcga gctggcgcgg
    13981 ggtgtagccc tggatcagca gcagcccgcc cggcctgagc gccgccgtca tgcaggcgaa
    14041 gagccgcgcc cgctcggccg gaccgacgaa ctggatgaag atcgccgcga ccacgtcgta
    14101 agccgacacc ggccagtccc aggtgagcag gtcggcttcg atgaagttcg gcgtcacgcc
    14161 ccgcgccgcg gccaggcggc gggccttggc gatggccgcg ggggcaaact ccaccgcatc
    14221 cacctgcagg ccctgctcgg ccagccatac gccgttgcgt ccctccccgt ccgccaccga
    14281 cagggcgcgc tggccgggcg tcagccagcg cgcctgggcc gccaggaagg cgttgggcgc
    14341 ggtgccgaag acgtagtcgt cgccagcgtc ggcgtagcgg ctgttccagc cgtggcgggg
    14401 atcgtcggga cgcaccggat caggccgcgt ccaccgcggc ggcaatgagc gccttgatgg
    14461 ccgccgcatc cacgtccatc acctgcaccc gctggggcag ggcctcgatg ttctccaggc
    14521 cggcgggacg cgccggcttg cggcccaggg cctcgacgat ggtgtcctcg aacttggccg
    14581 gctgggcggt ttccagcacc agggtcggca ctgccggatc gcgccgttcc agcgccacct
    14641 tgaggccgtc cgcggtatgg gtgtcgatca ccgtggcgta gcgggcgtcg gccaggcgga
    14701 tggtggccag acggtcggca tggctgctgc ggccggagac gaagccgtag tcgccgatcc
    14761 gggcgaaggc cggcgtagcg gacaggtcga aggcgccccc cgcatccact gcggcccaca
    14821 gctcgcgcac cttggcgccg tcgcggccga ccaggtcgaa gacgaagcgc tcgaagttgg
    14881 aggccttgga gatgtccatg gaggggctgg aggtggcgtg ggtctcgctg ctggcgcggg
    14941 gacgatacac gccggtgcgg aagaactcgt cgagcacgtc gttctcgttg gtggcgagga
    15001 ccagctgggc caccggcagg cccatctggc gggcgatgtg gccggcgcag atattgccga
    15061 aattgcccga cggcaccaca aaggcgacct gctggtcgtt ggacgcggtg gccgcgaaat
    15121 agcccttgaa gtaatagacg atctgcgccg cgacccgcgc ccagttgatg gaattgaccg
    15181 cgccgatctt gtactgcgcc ttgaaggcgt ggtcgttgga gaccgccttc acgatgtcct
    15241 gggcgtcgtc gaacatgccg cgcacggcga tattgacgat gttgtcgtcc tgcagcgaat
    15301 acatctgggc gcgctggaag gcgctcatgc ggccgtgggg cgagagcatg aagacacgca
    15361 cgccgtgctt gccgcgcatg gcgtactcgg ccgccgagcc ggtgtcgcc
//
LOCUS       DAOQMM010000091        15385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1528_length_15385_cov_55.373516, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000091 DAOQMM010000000
VERSION     DAOQMM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15385
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1528_length_15385_cov_55.373516"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLH85_13365"
     CDS             <1..224
                     /locus_tag="PLH85_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65600.1"
                     /translation="ESLEDQARTLVRAVTMFRLSEGAQATPRLEAPPENVAVLPERSA
                     RPVVARASAPLPKVRRAVGGSTDEEWEEF"
     gene            242..1162
                     /locus_tag="PLH85_13370"
     CDS             242..1162
                     /locus_tag="PLH85_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNB65601.1"
                     /translation="MTVQPPVRRSGAVPAPSRDARPAPTGTSSLQARDREFDFSPADF
                     ELVRSLIYKHAGITLSPIKQDMVYSRLARRLRARGDRTFAAYLERAQREPEEWETFVN
                     SLTTNLTSFFREAHHFDILAERLKKGGSRTPVKIWCSAASTGEEPYSLAITACEAFNT
                     MTPPVQIVASDIDTHVLATAQKGVYGIERLERLSRERVQRFFLRGTGTQEGYARVRPE
                     LQRLVEFKRINLLDAGWPLQGPFDYLFCRNVMIYFDKPTQHAILKRFAPLLKAEGLLF
                     AGHSESFLHAADVFRSLGRTVYERADRRAS"
     gene            1134..1763
                     /gene="cheD"
                     /locus_tag="PLH85_13375"
     CDS             1134..1763
                     /gene="cheD"
                     /locus_tag="PLH85_13375"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HNB65602.1"
                     /translation="MKGLTAVHPDPGYIEHLSTNRYFDRTFGCDAVKVLPGEYFVTTS
                     DIVLVTVLGSCVTACVRDREKGVGGMNHFMLPEGGAEGMLSTSARYGAYAMEVLLNHL
                     LKMGARRTNLEAKVFGGGRVMASLQQSQVGEKNAAFVLDYLATERIPVVAEDLLDIYP
                     RKVYFFPASGRVMMKKLVSVRNNTVLEREREYEARLRRDNLQGDVELFG"
     gene            1801..2895
                     /locus_tag="PLH85_13380"
     CDS             1801..2895
                     /locus_tag="PLH85_13380"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNB65603.1"
                     /translation="MAIKVLVVDDSALMRSVLTEIINSAPDLEVVGGAPDPLVAREMI
                     KTLNPDVLTLDVEMPKMDGLDFLARLMRLRPMPVVMISSLTQRGSETTLQALELGAVD
                     FVPKPKLDSLGGIESCRVEICEKIRGAYGSRPRVPVAFIAPSKPLPGEKSSGFAGRSG
                     GSEGGAGLDKLLCIGASTGGTEAIKEVLCSLPEKMPGILMVQHMPEMFTASFAKRLDG
                     LCSLNVKEAEHGEKVLPGTAYLAPGHSHLSVRRVSGGYVCELSRSEPVNRHRPAVDVL
                     FHSAAKQAGANALGVILTGMGKDGAQGLLAMRQAGAWTLGQDQDSCVVYGMPREAAQI
                     GALEEVAALKDIGARVLARLRGGERRGMRA"
     gene            2955..3260
                     /locus_tag="PLH85_13385"
     CDS             2955..3260
                     /locus_tag="PLH85_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB65604.1"
                     /translation="MDTNVSTQQSQARIQLSGRFDFNSHREFRDAVDKALGSPGTTEV
                     AVDLGNVAYLDSSALGMLLMLRDKAKTSGKTVCLANAKGSVRQVLDIANFGKLFPLV"
     gene            3260..4999
                     /locus_tag="PLH85_13390"
     CDS             3260..4999
                     /locus_tag="PLH85_13390"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HNB65605.1"
                     /translation="MSSEQSPESLFILVVDDNPDDLYLTRRDLEALGHRVALASTARE
                     ALALIEEAYPDVLMTDVGLPGMDGIELLQTVRARADGSWIPSLVFTGSLDEATQMRAL
                     EAGADAYLCKPASRKMLRAKLGVILRLRAMQRQAEARNKELAHYRVLQEEEQRLARFV
                     MERLINREALGDPALHHWIHSAAEFSGDLIAAARTPGNVLHVLLADGAGHGLAASLNV
                     LPVTAPFYRMTARGFGIDAIAREINNKVRALLPVDRFIAATLVAVNFRERYVTVWNGG
                     NPGPLLLRPDGAVHMHFNRHHLPLGVADEDEFDATVDAHVVDPRDHLLLFSDGLIELE
                     DASGRPLGMEGLGDALAGVLARDRLPRLTEIVHRMIGGKPPPDDISVAQIALHDEPHA
                     LPSAGSGEPTGPIVASDWRLVLRLGATDLRRLDAVPMLLGAIDHFDSVRPFAGRLFLV
                     LSELFNNALDHGLLRLPSSLKAGADGMDLYLEERARRLAELTAGAVEFELVLRQTPSL
                     SPALHITCADSGPGFNHEELVEAARGEPSDEDLLKSHGRGIPLLLKVCEDVRYRGPGN
                     RVEVVVRLSERAG"
     gene            5214..6893
                     /locus_tag="PLH85_13395"
     CDS             5214..6893
                     /locus_tag="PLH85_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65606.1"
                     /translation="MNIQLIAGLAGIGSAAGAAGIGVAFGVPLAPGFAAACSALGLAL
                     GGATYGLLRAVCVTPLRSLRTTIEDMHRDGDLSRRASVPHGYAEDLARVFNELIESFQ
                     GIVGKVIFDAQRVAEAAGTLSGQAGAVAQGSEEQRGAAESMVGAIEQMTAGVNAVAGH
                     ASQTASNAQVARDLSVQGVEIVTQASREIERIAQSVADSARVIGALGERSQAISGIVK
                     VIREIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTSTATTEIGGMIDAI
                     QAETRSAISAIDAGSKQAHAGADLARRAAESLRQINEGARETMERIDEIAEAITSHSH
                     DADQIVGNVRQIMLMVDRNSGEARETLDEAGSLNGLATNLKEISRVFKLGPAGEQAMQ
                     AHAKMPDMVRSVAAQIGELLEQAVSSGQVQVDDLFDENYRAIANTRPQKYNTRFDALT
                     DRLFPTIQEALLAKHPEMVYAIGTDRNGYVPTHNKRFSQPLTGDYNRDFTGNRSKRIF
                     DDPVGKQCGRHEMPFLIQTYRRDTGEIMHDISAPVYVQGRHWGGFRIGYKA"
     gene            7075..8043
                     /locus_tag="PLH85_13400"
     CDS             7075..8043
                     /locus_tag="PLH85_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNB65607.1"
                     /translation="MSELLKNIDARTRLAGTNKLEILLFTLGVDARTGRRETFGINVF
                     KVREVMRTPPITAAPEMPAAVEGMVSLRGVLVPVVDLAKYAGMGADSAREIMIVTEYN
                     GHTQGFLVEAVDTILRLDWSQMRVPPDMMTANMGGLVTAVTELPGPRLVMMLDVEKVL
                     SETTRYDDDLMFKGIEPIKCEEPVTVYFADDSSVARKQIERTLDTLGVRYVGSVNGRA
                     AWDELQKLAHYAESAGRKVKDLLQMVLTDVEMPEMDGYILTKKIKSDPRFEGVPVVMH
                     SSLSGMSNQQLGRSVGVDEYVPKFEPQRLAEVLRRLVDRCQSARAA"
     gene            8059..9012
                     /locus_tag="PLH85_13405"
     CDS             8059..9012
                     /locus_tag="PLH85_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNB65608.1"
                     /translation="MDTVLAERGLLEAVDARTKLAGSNRMEILLFSLGTQEIFGINVF
                     KVREVSRTPFITKAPNMPDGVEGLISLRGNVIPVLALAKIMGIAAPGEPLGGSMMVTE
                     YSKRTLGFLVHEVDRIIRVEWDKVRAPDNVTTSGNHFITAITELADGKLVSILDVETI
                     LASTFGEAVVGQIHPLEGGHDVNVFFVDDSAVARKKIGETLDKLGVKHKHALNGLEAW
                     TRLEGMASHAQQRGVCLADELSLILVDAEMPEMDGYVLTRNVKSDARFEGIPVVMHSS
                     LSSEANRAMGKSVGVDSYVAKFDADILAETLRPLLLRAQRD"
     gene            9028..9426
                     /gene="cheY"
                     /locus_tag="PLH85_13410"
     CDS             9028..9426
                     /gene="cheY"
                     /locus_tag="PLH85_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNB65609.1"
                     /translation="MSDPKMKFLVVDDFSTMRRIVRNLLKELGFTNVDEAEDGQIALQ
                     KLNSLPFDFVVTDWNMPNMDGLTLLQNIRATPSLKHLPVLMITAEAKKENIIAAAQAG
                     ASGYIVKPFTAATLSEKLEKIFEKMGKKAA"
     gene            9457..10293
                     /gene="cheZ"
                     /locus_tag="PLH85_13415"
     CDS             9457..10293
                     /gene="cheZ"
                     /locus_tag="PLH85_13415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNB65610.1"
                     /translation="MSKRLKFDESGDSDELQALFDSMAGVAAPASPPPAPPKLEVVAP
                     ADKAGDDDDLQALFDSVSSAYTPPAVEGIASEHSCDVVFNRIGTMTRRLHDTLRELGY
                     DRSLQEAAHAIPDARQRLSYIAEMTEQAASKVLNATDIAKPIQDTVESGATALRARWD
                     QLYANQLSPDEFKALAGETRSFLGDLVQHSRATNAQLLEIMMAQDFQDLTGQVIKKVV
                     ELAQGLESQLLRVLIEAMPAERKAEAPEGLMNGPVISAVGRDDVVTSQEQVDDLLESL
                     GF"
     gene            10316..12256
                     /locus_tag="PLH85_13420"
     CDS             10316..12256
                     /locus_tag="PLH85_13420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNB65611.1"
                     /translation="MSDFSGMEDLLQDFLVEAGDLLSGVDNKLVDLERAPDDRGLLND
                     IFRGFHTIKGGAGFLNASELVTLCHLTENLFDKLRNGELAVTPETMDVILASTGAVRD
                     MFGYLEQGVQPAAADPQLIEDLRLAIAGALRPAGVVTSAPEVLPAVVAAPPTPVTPAP
                     GGADGPDWNALFDAVTSKAPATATAVVPAADPPPVHQSAEDLAAKSPEQVIKAAVGRR
                     ATDKPGASGPIGRREGERQRDNSIRVDTARLDQVLNLSGEIGLTKNRLTALRSDILNG
                     RADTETLHALDLAVSQLDLLVSDLQNAVMKTRMQPIGRLFQKYPRIARDLARNLGKDV
                     ELVLSGEETEIDKTMIEDLSDPIIHLIRNAVDHGVEETSERRANGKPEKSIVRLEARQ
                     EGDHIVILVADDGRGMNAERLRAKALEKGLISDEEANTMDERQSYNLVFLPGFSMASK
                     VSDVSGRGVGMDVVRTNIQKLNGSIDIRSSLGKGTTFIISLPLTLAILPVLLVRLGDQ
                     PFAVPLSMVREILPIEPAEVQEVGGRATMVVRGEVLPIMPLATLLGWDQVQTPEYGVL
                     MQSAEQSFILAIDSFAGREDAVIKSLDDFRPKGVAGVTTLSNGQIVLILDMKELLGAL
                     GDTRGISRRALLAHLPVPALAA"
     gene            12418..12759
                     /locus_tag="PLH85_13425"
     CDS             12418..12759
                     /locus_tag="PLH85_13425"
                     /inference="COORDINATES: protein motif:HMM:NF022777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HNB65612.1"
                     /translation="MGGFEFDDWRFLAENDPAAFFRARERTLRQFITQHPDQRDALGA
                     LQMRIDTARAMSGTPQQACRDLFGLLEEHLLLLSERLADLRREATTLQRMLGVDGAEL
                     AVESPSVRGHS"
     gene            12812..13963
                     /gene="flhB"
                     /locus_tag="PLH85_13430"
     CDS             12812..13963
                     /gene="flhB"
                     /locus_tag="PLH85_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNB65613.1"
                     /translation="MADDSDLEKTESASARRLEQAREEGQVPHSRELATFIALMAGVT
                     ALYVLGDWAGQRMLHLFRSGLTLERSRAFAPDAMGQIFEQLALDALLTLAPLFVVLIV
                     AALVTPFLMGGWVFTTKVLTFDVSRLDPLQGLGRMFSTHGLAELVKASLKSVIVASVG
                     VWVVWRERDHLFALMLQPVEESVGEFASLLLLSSLLIVVGLGIIAAMDVPYQLWEYHR
                     KLRMTKEELRQEMKEQEGDPQIKARIRAAQREMSRRRMMSQVPKADVVVTNPTHYSVA
                     LHYDPDKAGAPVVVAKGVDLVALNIREVAREHGIPILEAPPLARALYAHCELEDPIPA
                     TLYTVVAEVMAYIFQLNHWIAEGGLPPEVPTGLTVPEGLDPAGTGEVSA"
     gene            14104..14568
                     /locus_tag="PLH85_13435"
     CDS             14104..14568
                     /locus_tag="PLH85_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65614.1"
                     /translation="MTAAGAAVGLVSGFSAGLREGRAHDPAVRPTAPFCRRRTGCRGT
                     RDGRGPTLTSGAARAGCFGDDRFKVALDGQVICACIVGACGTHGPEAIGAGSGAPHYG
                     PSDRFASSGSDATYAVNLAPGDLVASTHTLDFVAPSAEGGRPVGTNASRSTR"
     gene            14877..>15385
                     /locus_tag="PLH85_13440"
     CDS             14877..>15385
                     /locus_tag="PLH85_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNB65615.1"
                     /translation="MNDSLNLRALFAPANIRQLAGPLLVVLILAMMVLPLPAFVLDLM
                     FTFNIAVSVMVMLVAMYTRKPLDFSVFPTVLLVTTLLRLSLNVASTRVVLLEGHTGPD
                     AAGKVIEAFGHFLVGGNTAVGLVVFIILTVINFVVITKGAGRIAEVGARFTLDAMPGK
                     QMAIDADLNA"
BASE COUNT         2639 a         4978 c         5094 g         2674 t
ORIGIN      
        1 cggagagtct ggaggatcag gcccgcacgc tggtgcgggc ggtcaccatg ttccggctca
       61 gcgaaggcgc tcaggcgacg ccacgactcg aggcgccgcc agaaaacgtg gcggtgctcc
      121 cggagcggtc cgccagaccg gtagtggccc gcgccagtgc gcccctgccc aaggtgcgtc
      181 gggccgtggg cggctcgacc gacgaggaat gggaagagtt ttgagctgag cggatgctgc
      241 catgactgtc caaccacccg tgcggcgttc cggtgccgtg cccgcgccgt cgcgggatgc
      301 ccgcccggcg ccgaccggca cctcctcact gcaggcccgc gaccgggagt tcgatttctc
      361 gccggccgac ttcgagctgg tgcgttcact gatctacaag cacgccggca tcaccctgtc
      421 gccgatcaag caggacatgg tgtacagccg acttgcccgg cgtctgaggg cgcgtggtga
      481 ccggaccttc gcggcctacc tggagcgcgc ccagcgcgag cccgaggagt gggaaacctt
      541 cgtcaacagc ctgacgacaa atctcacctc ctttttccgg gaggcccatc acttcgacat
      601 cctggctgag cgcctgaaga agggtggcag tcgcacaccg gtgaagatct ggtgcagtgc
      661 ggcctccacc ggcgaagagc cttattccct ggcgatcacc gcctgcgagg ccttcaacac
      721 gatgactccg ccggtgcaga tcgtcgcctc ggatatcgac acccacgtgc tggccaccgc
      781 ccagaagggc gtctatggca tcgagcgtct cgagcgtttg tcgcgggagc gcgtgcagcg
      841 ctttttcctg cgcgggaccg gaacccagga aggctacgcg cgggtacgtc ccgagttgca
      901 gcgcctggtg gaattcaagc gcatcaacct gctggatgcg ggctggccct tgcaggggcc
      961 attcgattac ctgttctgcc gcaacgtgat gatctatttc gacaagccca cccagcacgc
     1021 catcctgaag cgtttcgcgc cgctcctgaa ggcggagggc ctgctctttg ccggacattc
     1081 cgaaagcttc ctgcacgcgg ccgatgtgtt ccgctcattg gggcggaccg tctatgaaag
     1141 ggctgaccgc cgtgcatcct gatccggggt atatcgagca tctgtccacc aaccgctatt
     1201 tcgaccggac cttcgggtgc gatgcggtga aggtgctgcc gggagaatat ttcgtcacca
     1261 cgtcggacat cgtcctggtg acggtgctgg gctcttgcgt gacggcctgt gtgcgcgacc
     1321 gggagaaggg cgtggggggc atgaatcatt tcatgctgcc cgaaggtggc gcggagggca
     1381 tgttgtccac gtcggcacgc tatggcgcat atgccatgga ggtgctgctc aatcacctgc
     1441 tcaagatggg ggcacgccgg acgaacctgg aggccaaggt gttcggaggc ggcagggtca
     1501 tggccagcct gcagcagtcc caggtgggcg agaagaatgc agccttcgtc cttgactatc
     1561 tggccaccga gcggattcca gtggtggccg aggatctgct cgacatctat ccgcgcaagg
     1621 tctatttctt tccggccagc ggccgggtga tgatgaaaaa gctggtgtcc gtgagaaaca
     1681 acacggtgct cgaacgtgaa cgcgaatacg aagcgcgtct cagacgcgac aatttgcagg
     1741 gcgacgtcga gctatttgga tgaccggaca tgccgtccgg gaactgactg gaagcgatcc
     1801 atggcaatca aggtgctcgt cgtcgatgat tccgcgttga tgcggagcgt gctgacggaa
     1861 atcatcaatt cggctcccga tctggaagtg gtgggtggag cacccgatcc tctggtggcc
     1921 cgggaaatga tcaagacgct caacccggac gtgctgaccc tcgacgtgga aatgcccaag
     1981 atggacgggc tcgattttct ggcccgtctg atgcggttgc ggccgatgcc ggtggtgatg
     2041 atttcgagcc tgacccagcg cggatcggaa accaccttac aggcgctaga actcggtgcg
     2101 gtggatttcg tgcccaagcc caaactcgat tcgttgggag gtattgaatc ctgtcgtgta
     2161 gaaatttgtg aaaagatcag gggcgcatat ggttcaaggc cgcgtgtgcc agtggcattt
     2221 atagcaccct ccaagccctt gcccggagag aaaagcagcg gatttgccgg tcgttccggc
     2281 gggtccgaag gtggggccgg tctggataaa ctgctctgta tcggtgcgtc gaccgggggc
     2341 accgaggcga tcaaggaagt gctatgttct cttccggaga aaatgcccgg aatcctgatg
     2401 gtgcagcaca tgccggagat gttcaccgca tcgttcgcaa agcggcttga cggcctgtgc
     2461 agcctgaacg tcaaggaggc cgagcatggg gaaaaggtgt tgccgggcac ggcctatctc
     2521 gccccggggc attcgcatct ttcggtgcgg cgtgtgtcgg gcggttacgt gtgtgaattg
     2581 tcccgatcgg aaccggtcaa tcgtcacagg cccgccgtag atgtgttgtt ccattcggcg
     2641 gccaagcagg cgggggccaa tgccctgggc gtgattctga cgggcatggg caaggatggc
     2701 gcgcagggtt tgcttgccat gcggcaggcg ggggcgtgga cccttggaca ggatcaggat
     2761 tcgtgtgtcg tgtatggcat gccccgggag gcggcgcaga tcggtgccct cgaggaagtg
     2821 gcggcgctga aggacatcgg cgcgcgggtg ctggcccgtc tccgcggggg agagcgtcgc
     2881 gggatgcgcg cgtgaagaat gaaggttgcc gggtggccgg cacggttgta cgtaacagtt
     2941 aatagaggca ttacatggac accaatgtca gcacccagca gagccaggcg aggattcagc
     3001 tctccgggcg tttcgatttc aattcccatc gggaattccg cgatgcggtg gacaaggcgc
     3061 tgggcagtcc cgggacgacc gaggtcgccg tggacctggg taatgtggcc tatctcgaca
     3121 gttccgccct tggcatgctg ctgatgcttc gtgacaaggc caagacgtcc ggcaagaccg
     3181 tctgtctggc caacgccaag ggcagcgtgc gtcaggttct cgacatcgcc aattttggca
     3241 agctctttcc gttggtttga tgtcttcgga gcaaagcccc gaatccctct tcatcctggt
     3301 cgtcgatgac aatccggacg acctctacct gacccgccgc gatctggagg ccctgggcca
     3361 ccgggtcgcg ctcgcgtcca ccgctcgtga agcgctcgcc ctcatcgagg aggcctaccc
     3421 ggacgtcctc atgacggacg tcggcctgcc cggcatggac ggcatcgaac tgctccagac
     3481 agtgcgtgcg cgggcggacg gtagctggat tcccagtctg gtctttacgg gcagtctcga
     3541 cgaggcgacg cagatgcgtg ccctcgaggc cggcgccgac gcctatctgt gcaagccggc
     3601 ctcgcgcaag atgctgcgcg ccaagctggg ggtgatcctg cgcctgcggg ccatgcaacg
     3661 gcaggccgag gcgcgcaaca aggaacttgc ccactaccgc gtcctccagg aagaggagca
     3721 gcgtctggcg cgcttcgtca tggagcgcct catcaaccgc gaggccctcg gcgatccggc
     3781 cctgcatcac tggatccatt cggcggccga gttcagtggt gaccttattg ccgcggcccg
     3841 tacgccgggc aacgtgctgc acgtcctgct ggccgacggt gccggtcacg gactggctgc
     3901 atccctcaat gtgctgcctg tcacggcacc gttctaccgg atgacggccc ggggcttcgg
     3961 catcgatgcc attgcccgcg agatcaacaa caaggtgcgc gccctgctgc cggtggatcg
     4021 cttcattgcg gcaaccctgg tggcggtcaa cttccgcgaa cgctacgtta ccgtgtggaa
     4081 cggcggcaat cccgggccgt tgctgttgcg cccggacggc gccgtgcata tgcacttcaa
     4141 ccgccatcac ctgccccttg gggtggcgga cgaggatgag ttcgacgcta ccgtggacgc
     4201 ccatgtggtg gatccccgtg accacctgct gctcttttcc gacggcctga tcgaactgga
     4261 ggatgcatcc ggacgcccgc tcggcatgga aggtctgggc gatgcgctgg ccggcgtgct
     4321 ggccagggat cgcctgccgc gcctgaccga gatcgtccat cgcatgatcg gcggcaagcc
     4381 cccgcccgac gacatctccg tcgcccagat cgcgctccac gacgagcctc acgccctgcc
     4441 gagcgcgggc tcgggggagc ccaccggccc gatcgtggcg agtgactggc ggctggtcct
     4501 gcgcctgggt gccacggatc tgcgccggct cgatgcggtg ccgatgcttc tcggcgccat
     4561 cgatcacttc gacagtgtcc ggccgttcgc cggccgcctg ttcctggtgc tgtcagaact
     4621 cttcaacaac gccctcgatc atggcctgct gcgccttccg tcgagcctca aggcgggcgc
     4681 cgacgggatg gatctgtacc tggaggagcg ggcccggcga ctggccgaat tgacggcggg
     4741 cgccgtggag ttcgagctgg tcttgcgtca aacacccagt ctgtcgcccg ccctgcacat
     4801 cacctgtgcg gattcagggc ctggcttcaa ccacgaggaa ctggtcgagg cggcccgtgg
     4861 cgagccgtcc gacgaggatc tgctcaagag ccatgggcgg ggtatccccc tcctgctcaa
     4921 ggtctgcgag gacgtccgct accgggggcc cggcaatcgg gtcgaggtgg tggtccgcct
     4981 gtctgagcgc gccggctgaa attttgtccg gctccgcccg gcggatactg tggttttcca
     5041 caaattgcgg cgtttttttt aagtttttgt gtagctgacc gataaggggc caaaccggcc
     5101 ccgccttgga gacgtgtctc agcgggtcgc gtccggtgcc gtgtgggccg ggtgggagcc
     5161 cttccgatgg aaggcgctcg attcaagacc attgacgagg tccgtcctcc atcatgaaca
     5221 ttcaactcat tgcaggactc gccggaatcg gaagcgcagc aggcgcggcg ggcatcggtg
     5281 tggccttcgg cgttccgctc gcgccggggt ttgcggcggc atgcagcgcg cttgggttgg
     5341 ccctgggtgg cgccacctac ggcctgctga gggcggtctg cgtcacgccg cttcggtccc
     5401 tgcggacgac catcgaggac atgcaccggg acggcgacct gtcgcgccgg gcgtccgtgc
     5461 cccatggcta cgccgaggac ctggcacggg tcttcaacga actgatcgaa agttttcagg
     5521 gcatcgtcgg caaggtcatc ttcgacgccc aacgggtcgc cgaggcggcc ggcaccctgt
     5581 ccggccaggc cggtgccgta gcccagggct ccgaggagca gcgtggcgct gccgaatcca
     5641 tggtgggcgc catcgagcag atgactgccg gcgtcaacgc ggtggccggc cacgccagcc
     5701 agaccgcgtc gaacgcccag gtggcccggg atctgtcggt tcagggggtg gagatcgtca
     5761 cccaggcgtc ccgcgagatc gaacgcatcg cccagtcggt ggccgattcc gcgcgggtca
     5821 tcggcgctct gggggaacgc tcccaggcca tcagcggcat cgtcaaggtg atccgcgaga
     5881 tcgcagatca gaccaatctg ctggcgctta acgcagccat cgaggcggcg cgggcaggcg
     5941 agcaggggcg cggttttgcc gtcgtggcgg acgaggtgcg caagctggcg gaacggacgt
     6001 ccaccgccac caccgagatc ggcggcatga tcgacgccat ccaggccgaa acccgcagcg
     6061 ccatttcggc catcgatgcg ggcagcaagc aggcgcacgc cggggctgac ctggcccgcc
     6121 gggcggccga gtccctgcgc cagatcaacg agggcgcccg cgagacgatg gaacgcatcg
     6181 acgagatcgc cgaggccatc acctcccaca gccacgatgc agatcagatc gtcggcaacg
     6241 tgcggcagat catgctgatg gtggaccgca actccggcga ggctcgggag accctcgacg
     6301 aggccgggag cctcaacgga ctggccacca acctgaagga aatcagccgg gtcttcaagc
     6361 ttggtccggc aggcgagcag gccatgcagg cccacgccaa gatgccggac atggtccgca
     6421 gcgtggctgc gcagatcgga gagctcctgg agcaggccgt gtcgtccggg caggtccagg
     6481 tcgacgacct gttcgacgag aactaccggg ccatcgccaa cacccggccg cagaagtaca
     6541 acacccgctt cgatgccctc accgaccgcc tcttcccgac gatacaggag gcgcttctgg
     6601 ccaagcaccc agagatggtc tacgccatcg gtaccgaccg caatggttac gtaccgaccc
     6661 acaacaagcg cttctcccag cccctgaccg gcgattacaa ccgggatttc acgggcaacc
     6721 ggagcaagcg gatcttcgac gatccggtcg gcaagcagtg cggccgccac gaaatgcctt
     6781 tcctgatcca gacctaccgg cgcgacacgg gcgagatcat gcacgatatc tcggcgccgg
     6841 tgtatgtaca gggccgtcac tggggcggct tccgcatcgg ctacaaggcc tgacgcgcga
     6901 cgatttgatg cacctccctg cgagtgttca gcccgccccg gcgggctgct tttttagtgg
     6961 tgcgcggggc tacggtaggt gccggattcc tcaagaactt ttggcacctg ccgttatggc
     7021 aacagcaggc ggccagggcc gatggaaggc caacgcgccg caacggagat caacatgtcc
     7081 gaacttctca agaacatcga cgcccgtacg cgtctggccg gcaccaacaa gctggagatc
     7141 ctgctcttca cgctgggagt cgatgcccgc accggccggc gcgaaacctt cgggatcaac
     7201 gtcttcaagg tgcgtgaggt gatgcgtacg ccccccatca ccgccgcacc ggagatgccg
     7261 gcggcggtgg aagggatggt cagcctgcgg ggtgtgctgg tgccggtggt ggatctggcc
     7321 aagtacgccg gtatgggcgc cgacagcgcc cgcgagatca tgatcgtcac cgagtacaac
     7381 gggcacaccc agggcttcct ggtggaggcg gtcgacacga tcctgcgcct cgactggtcc
     7441 cagatgcggg tgccgcccga catgatgacc gccaacatgg gcggactcgt gaccgccgtc
     7501 accgagctgc ccggccccag gctggtgatg atgctcgacg tggagaaggt gctgtccgag
     7561 acgacccgtt acgacgatga cctgatgttc aagggtatcg agcccatcaa gtgcgaagag
     7621 ccggtgacgg tgtatttcgc cgatgactcg tcggtcgccc gcaagcagat cgagcggacc
     7681 ctcgatacgc tgggcgtgcg ttacgtcgga tcggtcaatg gccgggcggc ctgggacgaa
     7741 ttgcagaagc tggcccacta cgccgaatcg gccggccgca aggtcaagga tcttctccag
     7801 atggtgctga ccgacgtgga gatgccggaa atggacggct acatcctcac caagaagatc
     7861 aagtcggacc cgcgtttcga gggcgtcccg gtggtgatgc attcatccct gtcgggcatg
     7921 tccaaccagc agctgggacg atctgtcggc gtcgacgaat acgtgcccaa gttcgagccg
     7981 caacggctgg ccgaggtgct gcggcgcctc gtcgaccggt gtcaatccgc ccgcgcggcg
     8041 tgagtctgga ggttcccgat ggatacggtt cttgccgaaa gaggtctcct cgaggccgtc
     8101 gatgcccgca ccaagctggc gggctccaac cgcatggaaa tcctgctgtt ctccctggga
     8161 acgcaggaga tcttcggaat caacgtgttc aaggtgcgcg aagtgtcgcg cacgcccttc
     8221 atcaccaagg cgcccaacat gcccgatggc gtggaagggc tgatctccct gcggggcaat
     8281 gtgatcccgg tgctggcgtt ggccaagatc atgggcatcg cggcgcccgg cgagcccctt
     8341 ggcggttcca tgatggtcac cgaatacagc aagcgcaccc tgggtttcct ggtgcacgag
     8401 gtggaccgga tcatccgggt cgaatgggac aaggtccgcg cgccggacaa cgtcaccacc
     8461 tccggcaatc atttcatcac cgccatcacc gaactggccg acggcaagct ggtgtccatc
     8521 ctcgatgtgg agaccatcct cgcgtccacc ttcggcgagg ccgtggtggg gcagatccat
     8581 ccgctggagg gcggccacga cgtcaacgtg ttcttcgtgg atgattccgc ggtcgcccgc
     8641 aagaagatcg gtgaaaccct ggacaaactc ggggtcaaac acaagcacgc cctcaacggc
     8701 ctggaagcct ggacccgtct tgaaggcatg gcgtcccacg cccagcaacg gggcgtctgt
     8761 ctggccgacg aactcagcct gatcctcgtc gatgcggaaa tgccggagat ggacggctac
     8821 gtcctgaccc gcaacgtcaa gtccgatgcc cgcttcgagg gcattccggt cgtcatgcac
     8881 tcgtccctgt cctcggaggc caaccgggcc atgggcaaga gcgtcggggt cgattcctac
     8941 gtcgccaagt ttgatgcaga catccttgcc gaaacccttc gcccgctgtt gctccgggcg
     9001 caacgggact gaacggagac cacccacatg tcggatccca aaatgaaatt tctcgtcgtc
     9061 gacgatttct ccacgatgcg ccgcatcgtg cgcaacctgc tcaaggaact gggcttcacc
     9121 aatgtggatg aggccgaaga cggtcagatc gcgctgcaga agctcaattc gctgcctttc
     9181 gatttcgtcg tgaccgactg gaacatgccc aatatggacg ggctgaccct gctccagaac
     9241 atccgcgcga cgcccagcct caagcacctg ccggtgctga tgatcaccgc cgaggccaag
     9301 aaagagaaca tcatcgccgc cgcccaggcc ggcgccagcg gctacatcgt caagccgttc
     9361 actgccgcga ccctgtccga aaagctggaa aagatcttcg aaaagatggg caagaaggcg
     9421 gcctgagcgg cgcccgcacg aaggagcgtt tcaaccatgt caaagcgtct caaattcgac
     9481 gaatcgggcg acagtgacga acttcaggcc ctgttcgaca gcatggccgg agtggccgcg
     9541 ccggcatctc cgccgccggc cccgcccaag ctggaagtgg tggcgccggc cgacaaggcc
     9601 ggtgacgatg acgatctgca ggccctcttc gactccgtat cgagcgccta cacgccgccg
     9661 gcggtggagg gcatcgcgtc ggagcacagc tgcgacgtgg tcttcaaccg catcgggacg
     9721 atgacccggc gcctgcacga cacgctgcgc gagcttggct acgaccgcag cctgcaggag
     9781 gccgcccacg cgattccgga tgcccgccag cgcctcagtt acatcgccga gatgaccgag
     9841 caggcggcga gcaaggtgct caacgccacc gatatcgcca agccgatcca ggacaccgtg
     9901 gaatccgggg ccaccgccct gcgggcgcgc tgggatcagc tctacgccaa ccagctgtca
     9961 ccggatgaat tcaaggccct ggcgggagag acccgcagct tcctcggcga tcttgtccag
    10021 cattcccggg ccaccaacgc ccagttgctg gagatcatga tggcccagga tttccaggat
    10081 ctcaccgggc aggtcatcaa gaaggtggtg gagcttgcgc agggcctgga gagtcagctg
    10141 ctgcgcgtgc tgatcgaggc catgccggcc gaacgcaagg ccgaggcgcc ggaaggcctg
    10201 atgaacggtc cggtcatcag cgccgtgggc cgtgacgacg tggtcaccag ccaggaacag
    10261 gtggacgacc tcctggaaag cctgggcttt tgatcatccg caaggagcga cgacaatgag
    10321 cgatttcagc ggaatggaag atctgttgca ggatttcctc gtggaagcgg gggacctgct
    10381 gtcgggggtg gacaacaagc tggtggatct cgaacgggcg cccgacgacc gggggctgct
    10441 caacgacatc ttccggggct tccacaccat caagggcggc gcgggcttcc tcaacgcgtc
    10501 cgagctggtc accctgtgcc acctcaccga gaacctcttc gacaagctgc gtaacggcga
    10561 actggccgtg acgccggaga ccatggacgt gatcctggcc tccaccggcg cggtgcgcga
    10621 catgttcggc tacctggagc agggcgtgca gccggcggcg gccgacccgc agctgatcga
    10681 ggatctgcgt ctggccattg ccggtgcgct gcgtccggcc ggcgttgtca cgtcggcgcc
    10741 cgaggtcctg cccgccgtgg tggcggcacc gccgacgccg gtgacgccgg cacccggcgg
    10801 cgccgacggg ccagactgga acgccctctt cgatgcggtc accagcaagg cgccggcgac
    10861 ggcgacggca gtggtgccgg ctgccgatcc tccgcccgtg caccaatcgg cggaagatct
    10921 ggcggcaaag tcccccgagc aggtcatcaa ggccgccgtc ggtcggcggg ccaccgacaa
    10981 gcccggggcc tccggtccga tcggccggcg tgagggcgag cgccagcgcg acaactccat
    11041 ccgtgtggat acggcccgcc tcgaccaggt gctcaacctg tccggtgaga tcggtctcac
    11101 caagaaccgg ctcaccgcgc tgcgcagcga catcctcaac ggacgcgccg atacagagac
    11161 cctgcatgcc ctcgatctgg cggtgagcca gctcgacctg ctggtttccg acctgcagaa
    11221 cgcggtgatg aagacccgca tgcagcccat cggccgcctc tttcagaagt acccgcggat
    11281 cgcccgcgat ctggcccgca acctgggcaa ggatgtggag ctggtcctgt ccggcgaaga
    11341 gaccgagatc gacaagacca tgatcgagga tctctccgac cccatcatcc acctgatccg
    11401 caacgcggtg gatcacggcg tcgaggagac ctccgagcgc cgtgccaacg gcaagcccga
    11461 gaaatccatc gtacgtctcg aagcccgcca ggagggcgat cacatcgtga tcctggtggc
    11521 cgatgacggc cgtggcatga acgccgaacg gctccgcgcc aaggcgctgg agaaaggcct
    11581 catcagcgat gaggaggcca acaccatgga cgagcgccag agctacaacc tggtgttcct
    11641 gcccggtttc tccatggcca gcaaggtgtc ggacgtttcc ggccgcggcg tgggcatgga
    11701 tgtggtgcgg accaacatcc agaagctcaa cggttccatc gacatccgct cgtccctggg
    11761 caagggcacc accttcatca tcagcctgcc cctgaccctg gcaatcctgc cggtgctgct
    11821 ggttcgcctg ggggatcagc ccttcgcggt gccgctgtcc atggtccgcg agatcctgcc
    11881 catcgagccg gcggaggttc aggaagtggg cgggcgcgcc accatggtgg tgcgtggcga
    11941 ggtgctgccc atcatgccgc tggccaccct gctggggtgg gaccaggtgc agacaccgga
    12001 atacggggtg ctcatgcaga gcgcggagca gagcttcatc ctcgccatcg acagctttgc
    12061 cgggcgcgag gacgcggtca tcaagtccct ggacgatttc cgtcccaagg gcgtggccgg
    12121 tgtgacgacc ctgtccaacg gtcagatcgt cctgatcctc gacatgaagg agctgctcgg
    12181 cgccctcggc gacacccgcg gcatctcgcg acgggctttg ctggcgcatc tgccggtacc
    12241 ggcgctggcc gcttgatccg tcacgaccgc ccgttcgcgg gcggtcggtg tcacacaacg
    12301 ctacaaaatc ccgtgacgta gatcgctcta tgggaggccg tgcttcgtta gcatggcctc
    12361 gaatgccttg cgcacgcgac cgatggaggt cgcccggcgg tggggtggag agcgatcatg
    12421 ggtggtttcg agtttgacga ctggcggttt cttgcggaaa acgatcccgc ggccttcttc
    12481 cgcgcgcggg aacggaccct gcggcagttc atcacgcaac acccggacca gcgcgacgcc
    12541 ctcggtgccc tgcagatgcg catcgatacc gcccgtgcca tgtccggtac gccgcaacag
    12601 gcctgccgtg acctgttcgg tctgctcgag gagcacctgc tgctgctctc cgaacggctg
    12661 gccgatctcc gtcgggaagc gacgacgctg caacgcatgc tgggcgtgga cggcgccgaa
    12721 ttggcggtcg aatccccctc tgttcggggc cattcctgag cgttcggcgg cgcatcatgg
    12781 gcgtccctga gatgcccgcg acggcgcccc gatggccgac gacagcgacc tcgaaaagac
    12841 agaatccgcc tcagcgcgac gcctggagca ggcccgtgaa gaaggtcagg ttccccattc
    12901 ccgcgagctg gccaccttca tcgcgctgat ggccggcgtc acggcgctgt acgttctggg
    12961 cgactgggcg gggcagcgga tgctccacct gttccgcagc gggctgaccc tcgaacgcag
    13021 ccgtgccttt gccccggatg ccatggggca gatcttcgaa cagctggccc tcgacgcgct
    13081 tctcaccctg gcaccgctct tcgtcgtcct catcgtggcg gcgttggtga cgcccttcct
    13141 gatgggcggc tgggtcttta ccaccaaggt actcaccttc gacgtcagcc ggctcgatcc
    13201 cctgcagggg ctcgggcgca tgttttccac ccatggcctg gcggaactgg tcaaggccag
    13261 cctcaagtcg gtgatcgtcg ccagcgtggg ggtctgggtg gtgtggcggg agcgcgatca
    13321 cctctttgcc ctcatgctcc agccagtgga ggagagcgtc ggcgagtttg ccagcctcct
    13381 gttgctgtcg tcgctgctca tcgtggtggg cctggggatc atcgcggcca tggacgtgcc
    13441 gtaccaattg tgggagtacc accgcaagct gcgcatgacc aaggaggagc tgcgccagga
    13501 aatgaaggag caggagggcg atccgcagat caaggcgcgc atccgcgcgg cccagcggga
    13561 gatgtcccgc cgccgcatga tgagccaggt gcccaaggcc gacgtggtgg tcaccaaccc
    13621 gacccattat tcggtcgcgc tgcactacga tccggacaag gccggcgccc cggtggtggt
    13681 cgccaagggc gtggatctgg tggccctcaa catccgcgaa gtggcccgcg agcacggcat
    13741 ccccattctc gaggcgcccc ccctggcgcg cgccctctac gcgcactgcg agctcgaaga
    13801 cccgattccg gccacccttt acaccgtcgt ggccgaggtg atggcctata tcttccagct
    13861 caaccactgg atcgccgaag gcgggctgcc gccggaggtg cccaccggcc tgacggttcc
    13921 ggaaggcctg gatccggcgg gcaccgggga ggtgtctgcc tgaagggtgg ggcgtcgcag
    13981 cgcccgccgt gtgtccgggg ccccctgggc gatcatcgga ggtggcgccc gtcgggcgaa
    14041 tgatcccggt ctgcgccgcc gtgattcatc gcggggcgca ttcatcggga tgcccgtcat
    14101 cccatgacgg ctgcaggcgc ggcggttggc ctcgtctcag gcttttcggc cggtttgcga
    14161 gagggccggg cgcatgatcc ggccgtccgg ccaacggcgc ctttctgccg gcgccgcacc
    14221 ggttgccggg gcacgcggga cggcaggggc cccacgctga cgtccggtgc agcccgcgcc
    14281 gggtgcttcg gcgatgaccg cttcaaggtc gccctggacg gtcaggtgat ctgcgcctgc
    14341 atcgtcggtg cctgcggcac ccacggcccg gaagcgatcg gcgccgggtc gggcgcgccg
    14401 cattacggtc cttccgaccg gtttgccagc agcgggtccg atgccaccta tgcggtgaat
    14461 ctggcccctg gtgatcttgt cgccagcacc cacaccctcg atttcgtcgc cccgtcggcg
    14521 gagggcggcc ggccggtcgg gacgaacgct tcgcgctcga caaggtgagt gtgaacggcc
    14581 gcctcgggac gcccaacacg cctgcgcccg agccttcggt gttcgcgttg ctgggaatcg
    14641 gatggacgag ggtcgcgcag acccgccggt ctgagcgcca gggctgaagg gcctgaaggt
    14701 ctggagcgcg ctccagcgtc ccgcatcggc cgcggcatcg tctaccgggc gcagcggccg
    14761 tgtgcggtgt ggaatggccc gtcttcggtc cgtgggtcgt tccgccccga atagcccccc
    14821 ctttccgcct cagttccggc cttctcccgc ggcgtgtcgc gggcaccatg gcgggcatga
    14881 acgatagcct gaacctgcgt gcgctcttcg caccggccaa catccgccag ttggccgggc
    14941 cgctgctcgt cgtgctgatt ctggcgatga tggtcctgcc gttgccggcc ttcgtcctgg
    15001 acctgatgtt caccttcaac atcgccgtct cggtgatggt catgctggtg gccatgtaca
    15061 cccgcaagcc gctggatttc tcggtcttcc cgacggtgct gctggtcacc acgctgctgc
    15121 gcctgtccct caacgtggcc tccacccgcg tggtgctgct ggaaggccac accggcccgg
    15181 acgcggcggg caaggtgatc gaggccttcg gtcacttcct ggtggggggc aataccgcgg
    15241 tggggctggt ggtcttcatc attctcacgg tcatcaactt cgtggtgatc accaagggtg
    15301 ccggccgcat cgccgaagtg ggcgcgcgtt tcaccctgga cgccatgccc ggcaagcaga
    15361 tggccatcga cgccgacctc aacgc
//
LOCUS       DAOQMM010000092        15364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1530_length_15364_cov_60.840682, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000092 DAOQMM010000000
VERSION     DAOQMM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_15364_cov_60.840682"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..183
                     /locus_tag="PLH85_13445"
     tRNA            108..183
                     /locus_tag="PLH85_13445"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:141..143,aa:Trp,seq:cca)
     gene            209..562
                     /gene="secE"
                     /locus_tag="PLH85_13450"
     CDS             209..562
                     /gene="secE"
                     /locus_tag="PLH85_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNB65616.1"
                     /translation="MADKIKFALALVLLVAGVVGFYLLAEQPMVLRVLSVLVGVGACA
                     AVAWKTEPGQRFAVFAGETVAEAKKVAWPSRKETFQMTGIVFAFVIIMAVFLWVTDKT
                     LEWALYDLILGWKKS"
     gene            559..1092
                     /gene="nusG"
                     /locus_tag="PLH85_13455"
     CDS             559..1092
                     /gene="nusG"
                     /locus_tag="PLH85_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNB65617.1"
                     /translation="MSKRWYVVHAYSGFEKSVQRALTERVARSGMQDLFGQILVPVEE
                     VVEMKNGQKSITERKFFPGYVLVEMEMNDDTWHLIKSTPKVTGFVGGTANKPTPISEK
                     EVAKIMQQMQEGVEKPRPKVLFEVGEMVRVKEGPFTDFNGSVEDVNYEKSKIRVSVTI
                     FGRATPVELDFGQVEKT"
     gene            1188..1619
                     /gene="rplK"
                     /locus_tag="PLH85_13460"
     CDS             1188..1619
                     /gene="rplK"
                     /locus_tag="PLH85_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNB65618.1"
                     /translation="MAKKIIGYVKLQVPAGKANPSPPIGPALGQRGLNIMQFCKEFNA
                     ATQGVEPGLPIPVIITAFADKSFTFVMKTPPATILIKKAAGIQKGSSKPHTDKVGKIT
                     KAQCEEIAKTKMKDLTAADMEAAIRTIAGSARSMGITVEGL"
     gene            1622..2323
                     /gene="rplA"
                     /locus_tag="PLH85_13465"
     CDS             1622..2323
                     /gene="rplA"
                     /locus_tag="PLH85_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNB65619.1"
                     /translation="MAKLSKRVQALRAKVDRNKPYPLTEALGLVKECATAKFDESIDI
                     AVNLGVDAKKSDQIVRGSVVLPAGTGKSVRVAVFAQGEKAEAARAAGADVVGFDDLAE
                     QVKGGTIDFDLCIATPDAMRVVGALGQILGPRGLMPNPKVGTVTMDVTTAVKNAKAGQ
                     VQYRTDKAGLIHATVGRASFTVEALQQNINALVDALVKAKPAAAKGVYLRRIALSSTM
                     GAGVRVEPTSLGAQQ"
     gene            2549..3049
                     /gene="rplJ"
                     /locus_tag="PLH85_13470"
     CDS             2549..3049
                     /gene="rplJ"
                     /locus_tag="PLH85_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNB65620.1"
                     /translation="MSLNLDDKKAVVAEVSAQVANAQTIVVAQYAGIEVGDLTALRAK
                     ARASGVYLRVLKNTLARRAVADTPFAPLAEQLTGPLIYSISADPVAAAKVLADFSKTN
                     DKLVLKAGCYAGKLLDKEGVQALASIPSREELLAKLLGVMQAPVSGFAGLLAALAKKR
                     EEGAAA"
     gene            3100..3477
                     /gene="rplL"
                     /locus_tag="PLH85_13475"
     CDS             3100..3477
                     /gene="rplL"
                     /locus_tag="PLH85_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNB65621.1"
                     /translation="MAITKEDILEAVGAMTVMELNDLVKAFEEKFGVSAAAMAVAAPG
                     AGGAAAAAVEEQTEFTVMLLAAGDKKVEVIKVVRAVTGLGLKEAKDLVDGAPKPVKEG
                     IAKADADALKKQLEDAGAKVEVK"
     gene            3675..7808
                     /gene="rpoB"
                     /locus_tag="PLH85_13480"
     CDS             3675..7808
                     /gene="rpoB"
                     /locus_tag="PLH85_13480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNB65622.1"
                     /translation="MAYSYTEKKRIRKSFAKRVAVKNVPYLLATQLESYASFLQAETP
                     PAARATQGLQAAFNSIFPIASHSGNARLEFVQYHLGEPAFDVKECQQRGLTFASPLRA
                     RVRLVILDKEAAKETIKEVKEQEVYMGEIPLMTNTGSFVVNGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDYLYFRVDRRRKMPVTILLKAIG
                     MSPEDILATFHDFENFRLVEDGAEYALVPDRLRGEVAKFDVVDPASGKVLVTKDKRVT
                     AKHIRDLANASITTVNVGDDFLLGRALAANVVSADGELVARANDEITEDLLDRLRDAG
                     VSDIATLYINDLDRGSYISQTLRIDETADQWAARVAIYRMMRPGEPPTEDAVEALFQG
                     LFYLAERYDLSVVGRMKFNRRAYPERLDDKAPEWVKRFNSTVGRKDGEGPGTLSNEDI
                     LAVVGILVELRNGRGEIDDIDHLGNRRVRSVGELAENQFRAGLVRVERAVKERLGQAE
                     SDNLMPHDLINAKPISAAIKEFFGSSQLSQFMDQTNPLAEITHKRRVSALGPGGLTRE
                     RAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLALFARTNQYGFLETPYRKVIDGAV
                     TEQIDFLSAIEEGQYVIAQANAEVDETGRLTGDLVSCRNRGEFTLAIPETVQYMDIAP
                     GQIVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPFVGTGIERTVAVDSGTA
                     EQALRGGVVDYVDANRVVIRVHDAETQAGEVGVDIYNLIKYTRSNQNTNINQRPVVKV
                     GDVVARGDVIADGASTDLGELALGQNMLVAFMPWNGYNFEDSILISERVVADDRFTSI
                     HIEELTVVARDTKLGPEEITRDIASLGEAQLSRLDESGIVYIGAEVEAGDVLVGKVTP
                     KGETQLTPEEKLLRAIFGEKASDVKDTSLRVPSGMNGIVIDVQVFTREGIERDKRAQS
                     IIDDMLRGYKTDLADQMRIVERDAFERIERTIIGQPANGGPRRLSKGTVIAKDYLDSL
                     EHYSWFDIRMADEEMAAQMEAVRESLEKTRKDFDLAFEAKKKKLTQGDELPPGVQKMV
                     KVYLAVKRRLQPGDKMAGRHGNKGVVSKIVPVEDMPYMEDGTPMDIVLNPLGVPSRMN
                     IGQVLETHLGWAAKGLGNQIDRMLRSQANVQEVRGFIEKIYNDSGKAEDIAELSDTDI
                     RELAGNLRKGVPFATPVFDGATEEEIQGMLELAGLPSGGQVTLYDGRTGDPFERKVTV
                     GYKHVLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAY
                     TLQEMLTVKSDDVTGRTKVYENIVKGEHKIDAGMPESFNVLVKEIRSLAIDIDLDRY"
     gene            7854..12062
                     /gene="rpoC"
                     /locus_tag="PLH85_13485"
     CDS             7854..12062
                     /gene="rpoC"
                     /locus_tag="PLH85_13485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB65623.1"
                     /translation="MKSLLADLFKQSLPNEEEFDAITIGLASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRE
                     RMAHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLNRAQ
                     ILSEDDYLAKVEEYGDDFIALMGAEGIREMLRSLDLPHEVEKLRSELETTGSEAKIKK
                     ISKRLKVLEAFQSSGIKPDWMILEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELKAPDIIVRNEKRMLQESVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPQLKLHQCGLPKLMALELFKPFIFNKLELMGL
                     ATTIKQAKKMVESQEPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQ
                     LHPLVCVAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGDPIIVPSQDI
                     VLGLYYATREGVNAKGEGMAFSDVGEVKRAYESGQVSLHAKVIVRLKEWDLGPEGESI
                     EKITRHQTTVGRAILSEILPAGLPFSVLQKPLKKKEISKLINASFRRCGLKATVVFAD
                     KLMQAGFGLATRAGISIAVKDMLVPAAKHPLIHAAEQEVKEIAQQYTSGLVTDGERYN
                     KVVDIWGRAGDQVAKAMMDQLGQEDVTDRNGNATKQESFNSIYMMADSGARGSAAQIR
                     QLAGMRGLMAKPDGSIIETPITTNFREGLNVLQYFISTHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVIEEDCGTKNGFLMKALIEGGEVVEPLRDRILGRVTAEDLVHPETQ
                     ETVIEAGSLLSEDMVDLIESLGIDEVRVRTPLSCDTRYGLCSKCYGRDLGRGSLVNVG
                     EAVGVIAAQSIGEPGTQLTMRTFHVGGAASRAAAASSVEAKSAGVIRFTQNMRYVANA
                     KGEKIVISRSAEVVVADDMGRERERHKIPYGATLSVDEGTAIKAGTQLATWDPHTRPI
                     ITEYAGTVKFENVEEGVTVAKQIDEVTGLSTLVVIDAKRRGASTAKGVRPQVKLIGDN
                     GEEVKIAGTDHAVSITFQVGSLITVKDGQEVSVGDVLARIPQESAKTRDITGGLPRVA
                     ELFEARAPKDAGVLAEVTGTVSFGKDTKGKQRLVITEPDGTVHEFLIPKDKHVMVHDG
                     QVVNKGELIVDGPADPHDILRLQGVEALARYIIDEVQDVYRLQGVKINDKHIEVIVRQ
                     MLRRVVITDPGDTSFIREEQVERAEVLDENDRVTGEAKLPASYSHLLLGITKASLSTD
                     SFISAASFQETTRVLTEAAILGKRDELRGLKENVIVGRLIPAGTGLAYHRNRKAQSMG
                     EDLGEGHAWAPEVDASETENVQAG"
     gene            12193..12570
                     /gene="rpsL"
                     /locus_tag="PLH85_13490"
     CDS             12193..12570
                     /gene="rpsL"
                     /locus_tag="PLH85_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNB65624.1"
                     /translation="MPTINQLVRKGRQAPVSKSKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKRPKKS"
     gene            12604..13074
                     /gene="rpsG"
                     /locus_tag="PLH85_13495"
     CDS             12604..13074
                     /gene="rpsG"
                     /locus_tag="PLH85_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNB65625.1"
                     /translation="MPRRRDVPKREVLPDPKFGSQDVSKFINAIMQSGKKSVAERIVY
                     GAFENISAKGGKDPLEVFSAAVANVRPMVEVKSRRVGGANYQVPVEVRPARRMALSMR
                     WLREAARKRSEKSMAQRLAGELLEAAEGRGAAMKKREEVHRMAEANKAFSHYRF"
     gene            13187..15280
                     /gene="fusA"
                     /locus_tag="PLH85_13500"
     CDS             13187..15280
                     /gene="fusA"
                     /locus_tag="PLH85_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB65626.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILYYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMDMQFPEHRFNIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQATKYKVPRLAFVNKMDRSGANFFKVVDQMKT
                     RLKANPVPVVIPIGAEENFAGVVDLIKMKSIVWDDASQGMKFEYQDIPADLAETAQTW
                     REQMVEAAAEASEELMNEYLENGDLSEERVKEGLRQRTLACEIQPMLCGTAFKNKGVQ
                     RMLDAVIEFLPSPVDIPPVKGVDDDEKEVSRKADDAEKFSALAFKLMTDPFVGQLTFI
                     RVYSGVLNSGSTVLNSVKNKKERIGRILQMHANNREEIKEVLAGDIAACVGLKEVTTG
                     ETLCDPDAPIILERMIFPDPVIHVAVEPKTKSDQEKMGIALGRLAAEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFNVEANVGAPQVAYRECIKKAVEQEGKFVKQSGG
                     RGQFGHVWIKMEPSEPGKGYEFVDAIKGGVVPREYIPAVDKGLQDAIGSGVLAGFPVV
                     DVKFTLFDGSYHDVDSNENAFRMAASMAFKEAMKKASPTLLEPMMAVEVETPEDYMGN
                     VMGDLSSRRGIVQGMEDLPGGMKAVKAEVPLSEMFGYATSLRSLTQGRATYSMEFKHY
                     SEAPKNVAEAVINKK"
BASE COUNT         3071 a         4771 c         4827 g         2695 t
ORIGIN      
        1 tgaaggcggt cgtaccgtcg gcgccggcgt cgtggccaag atcatcgagt aattctcggt
       61 cgctgtatgc agggcgcccc gttggggcgc cctgcagtct ctgctgcagg ggtatagctc
      121 aattggcaga gcgtcggtct ccaaaaccga aggttggggg ttcgattccc tctgcccctg
      181 ccacgtttct ctcgggttca ttgctttgat ggccgacaaa atcaagtttg cgctggcact
      241 ggtgctactg gtggccggcg ttgtgggctt ctatctgctc gccgagcagc ccatggtgct
      301 gcgcgttctg tccgtgctgg tcggcgtggg cgcctgtgcg gctgtggcgt ggaagacaga
      361 gcccggccag cgcttcgcgg tgtttgccgg cgagaccgtt gccgaggcga agaaggtggc
      421 ctggccctcc cgcaaggaga ccttccagat gaccggcatc gtgtttgcct tcgtcatcat
      481 catggcggtg ttcctgtggg tgacggacaa gaccctcgag tgggcgcttt acgatctgat
      541 cctgggctgg aagaaatcat gagcaagcgt tggtatgtcg tccatgccta ttccggcttc
      601 gagaagtcgg ttcagcgcgc gctcaccgag cgggtggcgc gaagcgggat gcaggacttg
      661 ttcggccaga tcctcgttcc ggtcgaggaa gtggtcgaaa tgaagaacgg ccagaagagc
      721 atcaccgaac gcaagttctt cccgggctat gtgctcgtgg aaatggagat gaacgacgat
      781 acctggcacc tcatcaagtc tacgccgaag gtgaccggct tcgtcggtgg gacggccaac
      841 aagccgacgc cgatctccga gaaggaggtc gcgaagatca tgcagcagat gcaggagggg
      901 gtcgagaagc cccgtcccaa ggtgctcttc gaggtcggcg agatggtgcg tgtcaaggaa
      961 ggtcccttca ccgatttcaa tggttccgtc gaggacgtga actatgaaaa gagcaagatc
     1021 cgcgtgtcgg tgaccatttt cggtcgtgcg acgccggtcg agctggattt cggtcaggtc
     1081 gagaagacct gaccccgtcg gggcgccggg tgcgcccctt cgcccattca gggcgcgagg
     1141 agtgcggcat gtcgccgtac gcttgaactc aaccaaggag ttgccagatg gcaaagaaaa
     1201 tcatcggcta tgtgaagctg caggttcctg caggcaaagc caaccccagc cccccgatcg
     1261 gcccggcact cggtcagcgc ggcctgaaca tcatgcagtt ctgtaaggag ttcaacgctg
     1321 ccacccaggg cgtcgaaccc ggtctgccga ttccggtgat catcaccgcc ttcgccgaca
     1381 agagcttcac cttcgtgatg aagacgccgc cggccaccat cctgatcaag aaggccgcgg
     1441 gcatccagaa gggttcctcc aagcctcaca ccgacaaggt tggcaagatc accaaggccc
     1501 agtgcgaaga aatcgccaag accaagatga aggatctgac cgcggccgac atggaagccg
     1561 ccattcgcac catcgccggc tctgcccgca gcatgggcat caccgtggag ggcctgtgat
     1621 catggcaaaa ctgtccaagc gtgttcaggc cctgcgtgcc aaggtcgacc gtaacaagcc
     1681 ctatcccctg accgaggccc tgggcctggt caaggaatgc gccaccgcca agttcgacga
     1741 gtccatcgac atcgccgtca atctgggcgt ggatgccaag aagtccgacc agatcgtccg
     1801 gggctccgtg gtgctgccgg ccggtaccgg caagtccgtc cgcgtcgccg tcttcgccca
     1861 gggcgaaaag gctgaagcgg cccgtgccgc tggcgccgat gtggtcggtt tcgacgatct
     1921 ggccgaacag gtcaagggcg gcaccatcga tttcgatctg tgcattgcca cgcccgatgc
     1981 catgcgcgtc gtcggcgccc tcggtcagat tctcggcccc cgtggcctga tgccgaaccc
     2041 gaaggtcggc accgtgacca tggatgtcac caccgccgtg aagaacgcca aggccggtca
     2101 ggttcagtac cgtaccgaca aggccggtct gatccacgcc accgtgggtc gtgcctcctt
     2161 caccgtggaa gcgctgcaac agaacatcaa tgcgctggtc gacgcgctgg tcaaggccaa
     2221 gccggctgca gccaagggcg tgtacctgcg ccgtatcgcc ctgtcgtcca cgatgggcgc
     2281 gggcgttcgc gtcgagccca cttcgctggg cgcgcagcag taagaacttt gggccggggc
     2341 gcgcctctcg gggtgccctg gatcgtcaaa gaccgcaggc gcctcggcgg attcttccgc
     2401 cgaagcttaa ttttccagcc tgcgtagacg gtgtcccaga acaggattta tcgcgtacgc
     2461 gacgttcgcg ggcgcaaagc tcctgatcca ggtcgccgta ggtgcgacgg tctttccgtc
     2521 gtgtgttgac ttgataggag gaaagacctt gagtctgaat ctcgacgaca aaaaagccgt
     2581 agtggctgag gtgtcggcac aagtggcgaa cgcccagacc atcgtcgtgg cgcagtacgc
     2641 gggtatcgag gtcggcgacc tcaccgcgtt gcgtgccaag gcgcgtgcgt cgggtgtcta
     2701 cctgcgcgtg ctgaagaaca ctctcgcacg gcgcgcagtg gcggacacgc ccttcgctcc
     2761 gctggccgag cagttgaccg gcccgctgat ctacagcatc tccgccgacc ccgtggccgc
     2821 cgccaaggtg ctcgccgact tctccaagac caacgacaag ctggtcctga aggcgggttg
     2881 ctatgcaggc aagctgctcg acaaggaagg tgtccaggcg ctggcgtcca tcccgagccg
     2941 cgaagagctg ctggccaagc tgctgggcgt catgcaggcg ccggtgtccg gctttgccgg
     3001 tctgctggcc gccctggcga agaaacgcga agaaggcgcc gctgcctgat cgggcagccg
     3061 ccggctgatt gaacgaaatt cgaattggag taagttgaaa tggcaattac caaagaagac
     3121 atcctggaag ccgttggcgc gatgaccgtc atggaactga acgacctggt caaggcgttc
     3181 gaagagaagt tcggtgtgtc cgccgccgcc atggctgtgg ccgctccggg cgccggtggc
     3241 gctgccgctg ctgccgtgga agagcagacc gaattcaccg tgatgctgct cgccgccggt
     3301 gacaagaagg ttgaagtcat caaggtggtc cgtgccgtga ccggcctggg cctgaaggaa
     3361 gccaaggatc tggtggatgg cgctccgaag cccgtcaagg aaggcatcgc caaggccgac
     3421 gccgacgcgc tcaagaagca actcgaagac gcgggtgcca aggtcgaagt caagtaatac
     3481 cggcttcgcg cagggggctg gtgccttttt ggcaccggcc cttttgtgct ttctgttttc
     3541 cgtaaccgta aacctgccgc cagaaagcaa aaaagagcat tcgattcagg ttatagcgcc
     3601 tgaaatactg aatcaagttc gcgaattgct gtcttttgtc ttctgacgtc cgacttctca
     3661 gacccggagc aactatggcg tactcctaca ccgagaaaaa gcgcatccgt aaaagcttcg
     3721 ccaagcgcgt ggcagtaaag aatgtgccct atcttttggc cactcagctg gaatcctatg
     3781 cgtccttcct gcaggcggaa accccgccgg cggcgcgggc cacccagggg cttcaggctg
     3841 ccttcaactc gattttcccc atcgcgagcc acagcggcaa tgcccgcctg gaatttgtcc
     3901 agtatcacct gggcgagccg gccttcgatg tgaaggaatg ccagcagcgc ggcctgactt
     3961 tcgcgtcgcc gctgcgcgcc cgggtccgcc tggtcatcct ggacaaggaa gcggccaagg
     4021 aaaccatcaa ggaggtcaag gagcaggaag tgtacatggg cgagattccg ctcatgacga
     4081 acaccggctc cttcgtcgtg aacggtaccg agcgggtcat cgtgtcccag ttgcaccgtt
     4141 ctcccggcgt gttcttcgag cacgaccgcg gcaagaccca ctcctccggc aagctgctgt
     4201 tctcggcacg gatcatcccc tatcgtggct cctggctcga cttcgagttc gatcccaagg
     4261 actacctgta cttccgcgtg gaccgtcgcc gcaagatgcc ggtgaccatc ctgctcaagg
     4321 ccatcggcat gagcccggag gacattctcg ccaccttcca tgacttcgaa aacttcaggc
     4381 tcgtggaaga cggtgcagag tacgccctgg tgcctgatcg cctgcgtggc gaggtggcga
     4441 agttcgacgt ggtcgatccc gcgtccggca aggtgctggt caccaaggac aagcgcgtca
     4501 cggccaagca tatccgtgat ctggccaacg ccagcatcac caccgtgaac gtcggcgacg
     4561 atttcctgct cggccgcgcc ctcgcggcca acgtggtgag tgccgacgga gaactggtcg
     4621 cccgcgccaa cgacgagatc accgaagacc tgctggatcg tctgcgtgac gccggggtga
     4681 gcgacatcgc caccctgtac atcaacgatc tggatcgcgg ctcctacatc tcccagaccc
     4741 tgcgcatcga cgagacagcc gaccagtggg cggcccgcgt ggccatctac cgcatgatgc
     4801 gtccgggcga accgcccacc gaagacgccg tggaagccct gttccagggc ctgttctacc
     4861 tggccgagcg ttacgacctg tcggtggttg ggcgcatgaa gttcaaccgc cgcgcctatc
     4921 ccgagcgtct ggacgacaag gcgccggaat gggtcaagcg cttcaattcc acggtcggcc
     4981 gcaaggacgg tgaaggtccc gggaccctgt ccaacgaaga catcctggct gtcgtcggca
     5041 tcctggtcga actgcgcaac ggtcgcggcg agattgatga catcgaccac ctgggtaacc
     5101 gccgcgtgcg ttccgtcggt gaactggctg aaaaccagtt ccgcgccggc ctggtgcgtg
     5161 tcgagcgcgc cgtcaaggaa cgcctggggc aggccgagtc cgacaacctg atgccccacg
     5221 acctgatcaa cgccaagccg atcagcgcgg ccatcaagga gttctttggc tcaagccagc
     5281 tctcccagtt catggatcag accaacccgc tggccgagat cacccacaag cgccgtgtct
     5341 ctgcgctggg cccgggcggt ctgacccgcg aacgtgccgg cttcgaagtg cgcgacgtgc
     5401 atccgaccca ctacggtcgc gtgtgcccga tcgagacgcc ggaaggtccg aacatcggtc
     5461 tgatcaactc cctggccctg ttcgcccgca ccaaccagta cggcttcctg gaaaccccgt
     5521 accgcaaggt catcgacggc gccgtcaccg agcagatcga cttcctgtcc gccattgagg
     5581 aaggccagta cgtgatcgcc caggccaacg ccgaggtgga cgagaccggc cgcctcaccg
     5641 gtgatctggt gtcgtgccgc aaccgcggcg aattcaccct ggcgatcccg gaaaccgtgc
     5701 agtacatgga catcgcaccg ggccagatcg tgtccgtggc ggcatccctg atcccgttcc
     5761 tggaacacga cgatgcgaac cgtgcgctga tgggcgccaa catgcaacgt caggccgtgc
     5821 cttgtctgcg tcccgagaag ccgttcgtgg ggaccggcat cgagcgcacc gtggcggtgg
     5881 actctggcac cgccgagcag gccctgcgcg gcggcgtggt ggactacgtg gatgccaacc
     5941 gggtcgtgat ccgggtgcat gacgcggaaa cccaggcggg cgaagtcggc gtcgacatct
     6001 acaacctgat caagtacacc cgttccaacc agaacaccaa catcaaccag cgtccggtgg
     6061 tgaaggtggg cgacgtggtg gcccgcggtg acgtgatcgc cgacggcgcc tccaccgacc
     6121 tgggtgaact ggccctcggg cagaacatgc tcgtggcctt catgccttgg aacggctaca
     6181 acttcgaaga ctcgatcctg atctccgaac gcgtggtggc ggacgaccgc ttcacctcga
     6241 tccacatcga ggagctcacc gtcgtcgccc gcgataccaa gctgggaccc gaggaaatca
     6301 cccgcgacat cgcctctctg ggcgaagcgc agctgtcccg tctggacgaa tccggcatcg
     6361 tgtatatcgg cgccgaggtc gaagctggcg acgtgctggt gggcaaggtc acgccgaagg
     6421 gcgaaaccca gctcacgccg gaagaaaagc tgctgcgggc gatcttcggc gagaaggcct
     6481 ccgacgtgaa ggacacctcg ctgcgcgtgc cctccggcat gaacggcatc gtcatcgacg
     6541 tgcaggtctt cacccgcgaa ggcatcgaac gcgacaagcg cgcccagtcg atcatcgacg
     6601 acatgctgcg cggctacaag accgacctgg ccgaccagat gcgcatcgtg gagcgtgacg
     6661 ccttcgagcg gatcgagcgc accatcattg gccagcctgc caatggcggc ccgcgtcgtc
     6721 tgagcaaggg caccgtcatc gccaaggact acctcgattc cctcgagcac tattcctggt
     6781 tcgacatccg gatggccgac gaagagatgg ctgcccagat ggaagccgtc cgtgagtccc
     6841 tcgagaagac ccgcaaggac ttcgacctgg cctttgaagc caagaagaag aagctcaccc
     6901 agggtgatga actgcccccg ggcgtgcaga agatggtcaa ggtgtacctg gccgtgaagc
     6961 gccgcctgca gcccggcgac aagatggccg gtcgtcacgg taacaagggt gtggtctcca
     7021 agatcgtccc cgtcgaagac atgccctaca tggaagacgg cacgccgatg gacatcgtgc
     7081 tgaacccgct gggcgtgccg tcgcggatga acatcggtca ggtgctcgaa acccacctgg
     7141 gctgggccgc caagggtctg ggcaaccaga tcgaccggat gctccgcagc caggccaacg
     7201 tgcaggaagt gcgcggcttc atcgagaaga tctacaacga cagcggcaag gcggaagaca
     7261 tcgccgagct gtctgacacc gatattcgcg agctggccgg caacctgcgc aagggtgtgc
     7321 ccttcgccac gccggtgttc gacggtgcca ctgaagaaga gatccagggc atgctggaat
     7381 tggccggtct gccctccggc ggtcaggtca ccctgtacga cggacgcact ggcgacccct
     7441 tcgagcgcaa ggtgacggtc ggctacaagc acgtgctcaa gctgcaccac ttggtggacg
     7501 acaagatgca cgcccgttcc accggtccgt actccctcgt cacccagcag ccgctgggcg
     7561 gcaaggccca gttcggcggt cagcgtttcg gggaaatgga agtgtgggcc ctcgaagcct
     7621 acggtgccgc gtacaccctg caggaaatgc tcacggtcaa gtcggacgat gtgaccggcc
     7681 ggaccaaggt gtacgaaaac atcgtcaagg gcgagcacaa gatcgatgcc ggcatgccgg
     7741 aatccttcaa cgtgctggtc aaggaaatcc gctctctggc catcgacatc gaccttgacc
     7801 gctactgagc ggccggtcgg atcgatgtgt gctcaccctg gatggagtga ttgatgaaaa
     7861 gtttgctcgc tgatctgttc aagcaaagcc tgccgaacga ggaagagttc gacgccatca
     7921 ccatcggcct ggccagcccg gagacgatcc ggtcctggtc ctatggcgag gtgaagaagc
     7981 cggagacgat caactaccgg accttcaagc ccgaacgcga cggtctgttc tgcgccaaga
     8041 tctttggccc cgtgaaggac tacgagtgcc tctgcggcaa gtacaagcgc ctcaagcacc
     8101 ggggcgtgat ctgtgagaag tgcggcgtcg aagtgacgct gtcgaaggtg cgccgtgaac
     8161 gcatggcgca catcgagctg gcctcgcccg tggcccacat ctggttcctg aagagcctgc
     8221 cgagccgtct cggcatggtg ctcgacatga ccctgcggga catcgagcgg gtgctctact
     8281 tcgaagccta cgtggtcacc gatcccggca tgacccccct caaccgcgcc cagatcctgt
     8341 cggaagacga ctatctggcc aaggtcgagg aatacggcga cgacttcatc gccctgatgg
     8401 gcgccgaagg catccgcgag atgctgcgca gcctggacct gccccacgag gtggagaagc
     8461 tgcgctccga gctggaaacc accggctccg aagccaagat caagaagatc tccaagcgtc
     8521 tcaaggtgct ggaagccttc cagtcctcgg gcatcaagcc cgactggatg attctcgaag
     8581 tgctgcccgt gctgccgccg gacctgcgtc cgctggtgcc gctggatggc ggccgcttcg
     8641 cgacctccga cctgaacgac ctgtaccgcc gcgtcatcaa ccgtaacaac cgcctcaagc
     8701 ggctgctcga actgaaggcg ccggacatca tcgtccgcaa cgaaaagcgg atgctgcagg
     8761 agtccgtcga ctccctgctc gacaacggtc gccgcggcaa ggccatgacc ggcgccaaca
     8821 agcgcccgct caaatcgctg gccgacatga tcaagggcaa gggcggccgc ttccgtcaga
     8881 acctgctggg caagcgtgtc gactactccg gtcgttccgt gatcaccgtg ggtccgcagc
     8941 tcaagctgca ccagtgcggt ctgcccaagc tgatggcgct ggagctgttc aagcccttca
     9001 tcttcaacaa gctggagctg atggggctcg caaccaccat caagcaggcc aagaagatgg
     9061 tggaaagcca ggagcccgtg gtgtgggaca tcctcgaaga ggtgatccgc gagcacccgg
     9121 tgatgctgaa ccgcgccccg acgctgcacc gcctgggcat ccaggccttt gagccggtgc
     9181 tgatcgaagg caaggccatc cagcttcacc cgctggtctg tgtggccttc aacgccgact
     9241 tcgacggcga ccagatggct gtccacgtgc ccctgtccct ggaagcgcag atggaagccc
     9301 gcaccctgat gctggcgtcc aacaacgtgc tgtctcccgc taacggcgat ccgatcattg
     9361 tgccgtccca ggacatcgtg ctgggccttt actacgctac ccgcgagggc gtgaatgcca
     9421 agggcgaagg catggccttc tccgatgtgg gcgaagtcaa gcgcgcctac gaatccggcc
     9481 aggtgtccct gcacgccaag gtgatcgtcc gcctgaagga atgggacctg gggccggaag
     9541 gtgaatccat cgagaagatc acccgccacc agaccaccgt cggccgggcg atcctgtccg
     9601 agatcctgcc tgccgggctg cccttcagcg tgctccagaa gcctctgaag aagaaggaga
     9661 tctccaaact catcaacgcc tccttccgcc gttgcggcct gaaggcgacg gtggtcttcg
     9721 ccgacaagct gatgcaggcc ggtttcggcc tcgccacgcg cgcaggcatc tccatcgcgg
     9781 tgaaggacat gctggtgccc gccgccaagc atcccctgat ccacgccgcc gagcaggagg
     9841 tgaaggaaat cgcccagcaa tacacgtccg gtctggtgac cgacggtgag cgctacaaca
     9901 aggtggtgga tatctggggg cgcgccggtg atcaggtcgc caaggcgatg atggaccagc
     9961 tcggtcagga agacgtcacc gaccgcaacg gcaacgccac caagcaggag tcgttcaact
    10021 ccatctacat gatggccgac tccggtgcgc ggggttccgc cgcacagatc cgtcagctgg
    10081 cggggatgcg cggcctgatg gcgaagccgg acggctcgat catcgaaacc cccatcacca
    10141 ccaacttccg cgaaggcctg aacgttctgc agtacttcat ctcgacccac ggcgcccgaa
    10201 agggtctggc cgatacggcg ctgaagacgg cgaactccgg ctacctgacc cgtcgtctgg
    10261 tggacgtgac ccaggatctg gtggtcatcg aggaagattg cggcaccaag aacggcttcc
    10321 tgatgaaggc gctgatcgag ggcggtgaag tggtcgaacc gctgcgcgac cgcattctgg
    10381 gtcgcgtcac cgccgaggat ctggttcatc ccgagactca ggaaaccgtg atcgaggccg
    10441 gttcgctgct cagcgaggac atggtcgatc tgatcgagtc cctgggcatc gacgaggtgc
    10501 gcgtccgcac cccgctgtcc tgcgacaccc gttatggcct gtgctccaag tgctatggcc
    10561 gcgatctggg tcgtggttcc ctggtcaacg taggcgaagc ggtcggggtc atcgccgccc
    10621 agtccatcgg cgagccggga acccagctca ccatgcggac cttccacgtg ggcggtgcgg
    10681 cgtcccgggc agcggctgcc agctccgtcg aggccaagtc ggccggcgtg atccgcttta
    10741 cccagaacat gcgctacgtt gccaacgcga agggcgagaa gatcgtcatc tcccgttccg
    10801 ccgaggtggt cgtggcggac gacatgggcc gcgagcgcga gcgtcacaag atcccgtacg
    10861 gtgcgaccct gtccgtcgat gaaggcaccg ccatcaaggc cggcacgcag ctggccacct
    10921 gggacccgca cacccgtccg atcatcaccg agtacgccgg taccgtgaag ttcgagaacg
    10981 tggaagaggg cgtcacggtg gccaagcaga tcgacgaagt gaccggtctg tccaccctcg
    11041 tcgtcatcga cgccaagcgt cgcggcgcaa gcaccgccaa gggcgtgcgc ccgcaggtga
    11101 agctgatcgg cgacaacggc gaagaagtga agatcgccgg caccgatcac gctgtgtcca
    11161 tcaccttcca ggtcggctcg ctgatcaccg tgaaggacgg tcaggaagtg tcggtgggtg
    11221 atgtgctggc acgtatcccg caggagtccg ccaagacccg tgacattacc ggtggtctgc
    11281 cgcgcgtggc cgagctgttc gaagcccgcg caccaaagga cgccggcgtg ctggccgaag
    11341 tgaccgggac ggtctccttc ggcaaggaca ccaagggcaa gcagcgtctc gtgatcactg
    11401 agccggatgg cacggttcac gagttcctga tccccaagga caagcacgtg atggtgcacg
    11461 acggtcaggt ggtgaacaag ggcgagctga tcgtggacgg tccggccgat ccccacgaca
    11521 tcctgcggct gcagggtgtc gaggcgctgg cccgctacat catcgacgaa gtgcaggacg
    11581 tgtaccggct gcagggcgtg aagatcaacg acaagcacat cgaggtgatc gttcgccaga
    11641 tgctgcgtcg cgtggtcatc accgatccgg gcgataccag cttcatccgt gaagagcagg
    11701 tagagcgggc cgaggtgctg gacgaaaacg atcgcgtgac tggcgaagcc aagctgccgg
    11761 cttcgtacag ccacctgctg ctgggtatca cgaaggcgtc gctgtccacc gactccttca
    11821 tctccgcggc gtccttccag gaaaccaccc gcgtgctgac cgaggctgcg atcctcggca
    11881 agcgcgacga gctgcgcggc ctgaaggaaa acgtgatcgt cggtcgcctg atcccggcgg
    11941 gtaccggtct ggcctatcac cgcaaccgca aggcgcagtc gatgggtgag gatctgggcg
    12001 aaggccatgc ctgggcgccg gaggtcgatg cgtccgaaac cgagaacgtg caggccggtt
    12061 gacgtcaact tgacttgagc gatatactcc ggcctctttt tgcggactgg ccaatcgtag
    12121 tcagtcgcag ccggaataac catccgaggc ggtcctttcg ggctgcctcg gtgttttggg
    12181 aaattcttag ctatgccaac aattaatcaa ctcgttcgta agggccgtca ggcgcccgtc
    12241 tccaagagca aggttcccgc gctggaagcc tgcccgcaaa agcgcggtgt ctgcactcgt
    12301 gtgtacacca cgaccccgaa gaagccgaac tccgcgctgc gtaaggtggc aaaggttcgt
    12361 ctgaccaatg gcttcgaggt gatctcctac atcggtggtg aaggtcacaa cctccaggag
    12421 cactcggtcg tgctgatccg cggtggtcgt gtgaaggact tgccgggtgt gcgttaccac
    12481 atcgtgcgcg gttcccttga cctgcaaggc gtcaaggatc gcaagcagtc ccgctcgaag
    12541 tacggcgcca agcgtccgaa gaagtcctaa ttgtcggcaa tcacagaata gcgagattca
    12601 atcatgcccc gtcgtcgtga cgtacccaag cgggaagttc tgccggatcc gaagttcggt
    12661 tcccaggatg tttccaagtt catcaacgcc atcatgcaga gcggcaagaa gtccgttgcc
    12721 gagcgcatcg tgtatggcgc tttcgaaaac atctccgcca agggcggcaa ggatccgctg
    12781 gaggtgttct ctgcggctgt tgccaacgtt cgcccgatgg tcgaagtcaa gagccgtcgt
    12841 gtcggtggcg ccaactacca ggtgcccgtg gaggtgcgtc cggctcgtcg catggcgctg
    12901 tccatgcgct ggctccgtga ggcggcccgc aagcgctccg agaagtccat ggcccagcgt
    12961 ctggcaggcg agctgcttga ggcggccgaa ggtcgtggcg ctgcgatgaa gaagcgggaa
    13021 gaagtgcatc gcatggcgga agccaacaag gcgttctcgc actaccgttt ctgacctcca
    13081 ggaaatgacc ggtcgggccc tgcaaggggc tcgatttatt tggaacgtat cttcaagaag
    13141 ctcagcagtc cgggcttcgc aacttcagga aggctaggtt agtactgtgg cacggaagac
    13201 cccgatcgag cgctaccgta atatcggtat ctctgctcac atcgacgccg gcaaaaccac
    13261 cacgacggag cgcatccttt actacacggg tgtgaaccac aagatcggtg aagtgcatga
    13321 tggcgccgcc accatggact ggatggagca agagcaggag cggggtatca ccatcacctc
    13381 ggctgcgacc acctgtttct ggaagggcat ggacatgcaa ttcccggagc accgcttcaa
    13441 catcatcgac accccggggc acgtggactt cactatcgag gtggagcgct ccatgcgtgt
    13501 tctggacggt gcctgcatgg tttactgtgc ggtgggcggc gttcagcccc agtccgaaac
    13561 cgtgtggcgt caggcgacca agtacaaggt gccgcgtctg gccttcgtga acaagatgga
    13621 ccgctccggc gccaacttct tcaaggtcgt cgaccagatg aagacccgcc tgaaggcgaa
    13681 cccggtgccc gtcgtgatcc ccatcggtgc tgaagaaaac ttcgccggtg tcgttgatct
    13741 gatcaagatg aagtccatcg tctgggacga tgcctctcag ggtatgaagt tcgaatacca
    13801 ggacatcccc gcggatctgg ccgaaacggc tcagacctgg cgcgagcaga tggttgaagc
    13861 ggctgccgaa gcttctgagg agctcatgaa cgagtacctc gagaacggcg acctgtccga
    13921 agagcgcgtc aaggaaggtc tgcgtcagcg caccctggcg tgcgagatcc agcccatgct
    13981 gtgcggcacc gccttcaaga acaagggcgt gcagcgcatg ctcgacgcgg tcatcgagtt
    14041 cctgccttct cccgtggata ttccgccggt caagggcgtt gatgacgacg agaaggaagt
    14101 gtcccgcaag gcggacgacg ccgagaagtt ctctgcgctg gctttcaagc tgatgaccga
    14161 ccccttcgtc ggccagctga ccttcatccg cgtgtattcc ggcgtgctga actccggctc
    14221 cactgtgctg aactccgtca agaacaagaa ggagcgtatc ggtcgtattc tgcagatgca
    14281 cgccaacaac cgcgaggaaa tcaaggaagt gctggcgggc gacatcgcgg cgtgtgtggg
    14341 cctgaaggaa gtcaccaccg gtgaaaccct gtgcgatccc gacgcgccga tcattcttga
    14401 gcgcatgatc ttccccgacc ccgtgatcca cgtggccgtc gagcccaaga ccaagagcga
    14461 tcaggaaaag atgggtatcg ccctgggtcg tctggcagcc gaagatccgt ccttccgcgt
    14521 gcgcaccgat gaagagtccg gtcagaccat catctccggg atgggcgagc tgcacctgga
    14581 aatcatcgtc gatcgcatga agcgtgaatt caacgtggaa gccaacgtcg gtgcacctca
    14641 ggtggcctac cgcgaatgca tcaagaaggc ggtcgaacag gaaggcaagt tcgtcaagca
    14701 gtccggtggt cgcggccagt ttggtcacgt ctggatcaag atggagccga gcgagccggg
    14761 caagggctac gagttcgtcg atgcgatcaa gggcggcgtg gtgcctcgcg agtacatccc
    14821 cgcggtggac aagggcctgc aggacgcgat cggcagcggc gtgctcgccg gcttcccggt
    14881 ggtggacgtg aagttcaccc tgttcgacgg ctcctaccac gacgtggact ccaacgaaaa
    14941 cgccttccgc atggcggctt ccatggcttt caaggaagcc atgaagaagg ccagcccgac
    15001 cctgctcgag ccgatgatgg ccgtcgaggt ggaaacgccg gaagactaca tgggtaacgt
    15061 gatgggcgac ctttcgtccc gtcggggcat cgtccagggt atggaagacc tgcccggcgg
    15121 catgaaggcg gtcaaggcgg aggtgccgct gtccgagatg ttcggctatg ccacctcgct
    15181 gcgttccctg acccagggtc gcgccaccta ctcgatggaa ttcaagcact acagcgaagc
    15241 gcccaagaac gtcgctgaag ccgtcatcaa caagaagtaa tcgaaccctc agatcaggaa
    15301 gctgaaatca tggctaagga aaaatttgcg cggacgaaac cgcacgtaaa cgttggcacg
    15361 atcg
//
LOCUS       DAOQMM010000093        15244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1551_length_15244_cov_52.180986, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000093 DAOQMM010000000
VERSION     DAOQMM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1551_length_15244_cov_52.180986"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..638
                     /locus_tag="PLH85_13505"
     CDS             312..638
                     /locus_tag="PLH85_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB65627.1"
                     /translation="MSDTETLPDLEAIRQAASAATAVLSALANENRLLLLCQLSRGER
                     SVGELEAELDLHQPTLSQQLSVLRAENLVATRRDGKRVFYSIADVRVLALLDTLYEQF
                     CPRKDV"
     gene            644..1078
                     /locus_tag="PLH85_13510"
     CDS             644..1078
                     /locus_tag="PLH85_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNB65628.1"
                     /translation="MNIDWTHFTPQAALTGGALIGLATGLLLVLNGRIAGISGILGGL
                     LQRCSVSELSWRAAFVAGLVVAPALFGLFLPLPEIRVDARWGGLVVAGLLVGYGARLG
                     SGCTSGHGVCGLSRMSPRSLVATLTFMGTGFMTVFVVRHLLA"
     gene            1089..1541
                     /locus_tag="PLH85_13515"
     CDS             1089..1541
                     /locus_tag="PLH85_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65629.1"
                     /translation="MIVFTAFLAGLVFGIGLIVSGMANPSKVTGFLDLAGAWDPSLAF
                     VMAGAIALGVVGYRVALRRGRPVFGAALQIPTLRNIDRRLVVGSALFGIGWGIAGICP
                     GPGLVMLGAGLPEGLVFVSAMLVGMALFELQQGHLARLLAALGIARNS"
     gene            1562..1939
                     /locus_tag="PLH85_13520"
     CDS             1562..1939
                     /locus_tag="PLH85_13520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB65630.1"
                     /translation="MYSVQVDRSPVITVNGAKHAVSYAVDGSQMNPLEAFYATLAGCA
                     AVFAKKACKELGISPEGIHIACRPFAGAAGPLSLSKFKTEVRFPEGIDAAQKAAVLEA
                     IKHCAVKQIVEAGAAVDFQVAEG"
     gene            complement(1991..3325)
                     /locus_tag="PLH85_13525"
     CDS             complement(1991..3325)
                     /locus_tag="PLH85_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNB65631.1"
                     /translation="MSHPAHPLPELVSFLESLPEPHILCDRSYRILAANAAYRAKCAR
                     PEDLVGRTCYEVSHRYPVPCDRAGESCPLALSLQSGQRERVVHLHHTSSGERYEKIEL
                     SPVRDARGEIAYFIEKLEPLPVARGVAGGEGLIGRAATFRGMLELVARVAPSEATVLL
                     QGESGTGKELVARAIHDASRRADRPFVAVDCSGLTETLFESELFGHERGAFTGATVRK
                     VGLVEAASGGTLFLDEMGDIPLSMQVKLLRLLETGTYRRVGSTELVRADIRLISATHR
                     PLKSLVGDGRFRQDLFYRINTFPIALPALRERREDIPLLAEALLRRVAPDRRLGVAPD
                     ALDDLLAYDYPGNVRELRNILERASLLCDGDTLERRHLPDDLLPATALSASTASYAVT
                     TGGLPDDATLATLLRSFRGTRRALATHLGVSERTLYRRIREMDVADPPPGGA"
     gene            3675..4286
                     /gene="petA"
                     /locus_tag="PLH85_13530"
     CDS             3675..4286
                     /gene="petA"
                     /locus_tag="PLH85_13530"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNB65632.1"
                     /translation="MTCETWAPGACDPARRSLLRATAGAGSVLAVATAVPFVASLTPS
                     QRARAAGAPVEVDIDKLAPGELMVVEWRGKPVWILRRTPEMLASLARAEQHLADPGSA
                     VDAQQPDYARNAHRSIRPEIFVAVGLCTHLGCSPSSVFEPGHGALGADWPGGFLCPCH
                     GSTFDLAGRVYRNKPAPTNLEVPPHTFLSDTRLRIGADAGTPA"
     gene            4301..4600
                     /locus_tag="PLH85_13535"
     CDS             4301..4600
                     /locus_tag="PLH85_13535"
                     /inference="COORDINATES: protein motif:HMM:NF021140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon YbgE family protein"
                     /protein_id="HNB65633.1"
                     /translation="MSRRDADTGPHVPRDPMARALALLTGAFAAFLLLCFPQVLVRSD
                     GVADPWAAVCLIGAMITGLASGVGTPFERRWTRALLSPESCLLALTLATLRVAAH"
     gene            4611..5255
                     /locus_tag="PLH85_13540"
     CDS             4611..5255
                     /locus_tag="PLH85_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB65634.1"
                     /translation="MYFRQFFDPATSAMSYLLADPAARAAAILDPLGDPCQALVFRAL
                     LAEHALDLHLILLTHVHGNALEDIRSTYPDVRTVAGVGPVTGVSVAREGDRLHLGREE
                     IRIIATPGHTFHSVSYLWRDRLFCGDALELGGCAASVEGEGDAGQLFDSVTGRLFALA
                     DETLVFPGHDFNGRTVSTIGEERRRNPFFQSLNRDAFTAALRTRDAADRQFRWP"
     gene            5527..7155
                     /locus_tag="PLH85_13545"
     CDS             5527..7155
                     /locus_tag="PLH85_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65635.1"
                     /translation="MSVRFHSLKARLALLGLISVLGVCLLAALSLIQTTRIKANLVVF
                     ADRHIALNHHATRTYAQGLQMGQALRNIVLDPANRKAYENFAQASESFKEVFGALEPL
                     VKAEDAAAAEELAQRVAAWRPLQAQVLALVQGGEADAARALLTTRETPAWRAVREILL
                     AQVKRSDAAAEGARVSLLADVDTIAVGAAVVGVATLAVVLGATLWVGRAVFRQVGGEP
                     SATAASLARIAAGDLTGEIRPRAGDCDSILAAASDMQAHLAGLVRSIGRGAVSMMQAS
                     EAIARNAESVADTAERQSQATSGIAAAVEELTVSIGVMSDNAVHAASLSGQTEGKVQD
                     GLTQVSATTDTIRQVAEGMTAFAETMGELSSKVSGINGIVQAIHEIAEQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAERTATSTQEISAMIAGVQDSTRVALDNMARTRAV
                     ALEGARHMDQTQEAVAGLSTVAAGVREEVDAIRAALDEQRAASTDIARRVEQIAQGIE
                     GTHVSAAASSRSAQELVALAHELEAMTGRFRVSA"
     gene            7249..7851
                     /locus_tag="PLH85_13550"
     CDS             7249..7851
                     /locus_tag="PLH85_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB65636.1"
                     /translation="MGLRVRGAVLAACWVVATSAGAEDVKRAEEIVGGRCFLCHGMEG
                     EAASALYPRLASQHAEYIAKQLADFKSGRRKSDTMAGMVADLTPDEMKSLGLYFQAKP
                     ARHGEAGDADLAGVGRYIFTKGNPYSGVAACTACHGGKGHGTPQLPRLAGQQALYLEN
                     QLKQFNTRERTNDNAVMHSIASKLTELELKAVSAYISTLD"
     gene            complement(7928..9205)
                     /locus_tag="PLH85_13555"
     CDS             complement(7928..9205)
                     /locus_tag="PLH85_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB65637.1"
                     /translation="MTNRRDFLRSAALAGSAAAFPGLSGCALGSRPDGHVVVVGGGYG
                     GATLAKYLRMWSERSVSVTLIERNPQFISCPMSNLVIGGEKTLADITLSYDGLKHTWG
                     VQVVQDEVTAIDPTARTVRLASGGSLRYDRLVLSPGIEFMFDQVPGLESDAARSTVLH
                     AWKAGPQTVALRQQLADMRDGGVYALSIPKAPYRCPPGPYERACLVASYLQRNKPRSK
                     VLILDANEEIVSKKALFTKAFEKYKGILEYLPNSELREVDAASRTAVLEFDKVKADVL
                     NVVPPQRAGDVAAKSGVRLINNRWVEVDWLSFEAVGTPGVHVIGDALFPAPTMPKSGH
                     MANQHAKVAAAAILNLLAGQPPNPTPVVMNTCYSFVDPKNVIHVSSVHQYDAAKKVVL
                     PVPGAGGVSAAANELEGKYAHSWARNIWADMLT"
     gene            complement(9218..9532)
                     /locus_tag="PLH85_13560"
     CDS             complement(9218..9532)
                     /locus_tag="PLH85_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB65638.1"
                     /translation="MKLLQWSAVLCLAGTAGAALAADRPDPELARNLAATCANCHGTD
                     GRAVPGAGNEALAGVDKAKLLQKLQDFRSGAKPATIMHQIAKGYTDAQLDLIAAYFAA
                     RK"
     gene            9778..11124
                     /gene="soxC"
                     /locus_tag="PLH85_13565"
     CDS             9778..11124
                     /gene="soxC"
                     /locus_tag="PLH85_13565"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HNB65639.1"
                     /translation="MAEQPIQRGRLRPAPEHFLNEADVQAVEAGRRGFLRKALVTASA
                     AMAATTVARAEDGDPAILTLPPWTTSLGQPVAARPYGLPSQYEKGLQRRESPGLTRMS
                     QVSVAFTPLQGLFGIVTPSGLHFERHHQGWSDIDPSKHRLMINASDDGFIRAPRVYTV
                     DDILRLPPVSRMHFIECGANTGMEWGNVAVPTVQYTHGMLSCSEFTGVPLRTLLDDCG
                     VNWKKARYVLAEGADGSSMTRTIPIELVESGEVLVAYGQNGEMLRPENGYPLRLVVPG
                     VQGVSWVKWLRRIEVGDQPWATKDEAIHYVDLLPGGQHRQYTSIQECKSVITTPSGGQ
                     MLLDKGFYNISGLAWSGRGKITRVDVSTDGGVNWRSARIDGPVHGKCLTRFNIDWVWD
                     GRPAILQSRAVDDTGYVQPRYGQLRAVRGTKSIYHNNAIQSWKVAETGEVSNVQVL"
     gene            11108..12184
                     /locus_tag="PLH85_13570"
     CDS             11108..12184
                     /locus_tag="PLH85_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB65640.1"
                     /translation="MSRFSRIPLALVALVALGLAGTAVADGKFPGIGRPATAAEVRAW
                     DIDVRPDFKGLRPGSGSVDQGQEVWEAKCASCHGTFGESNEVHTPLIGGTTQDDIRTG
                     HVASLAKGDVPQRTAIMKVATVSTLFDYINRAMPLTAPKSLKNDEVYALLAYLLNLAE
                     VVPADFVLSNWNIAEVQQRMPNRKGMTTDHGMWPGASAARGGIGNGGTPDVKNTACMH
                     RCKDAVQVASLLPAYAETAHGNLAEQNRSVGPVRGKETGARAMADAKSVALSENAAMA
                     KLAGDSGCMACHGLASRVVGPGYNEVATRYTGQPDAEAKLAAKVKAGGQGVWGAIPMP
                     PQSQLKDGDVRSLVKWILSGAKAQ"
     gene            12213..12680
                     /gene="soxY"
                     /locus_tag="PLH85_13575"
     CDS             12213..12680
                     /gene="soxY"
                     /locus_tag="PLH85_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HNB65641.1"
                     /translation="MNMERREVLKAGSGLGLLGLLVAAGLITPGEARAEWAKAAFDAK
                     SIEDTLKALGAGKPDSSADIQITAPDIAENGAVVPVGVVTALPDVQVIALLVEKNPNP
                     LAASFTLPAGTEPSVQTRVKMGQTSDVYAVVKAGGKFLMTKKEIKVTLGGCGG"
     gene            12703..13014
                     /gene="soxZ"
                     /locus_tag="PLH85_13580"
     CDS             12703..13014
                     /gene="soxZ"
                     /locus_tag="PLH85_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HNB65642.1"
                     /translation="MADPMKIRASAKDGMTEVKVLMSHEMETGQRKDASGALVPAHFI
                     TEVTAEHNGRPVLAAQFGTAVSKNPYLAFKFKGGAKGDKLTVSWVDSKGDTRRDEVLI
                     A"
     gene            13095..13901
                     /gene="soxA"
                     /locus_tag="PLH85_13585"
     CDS             13095..13901
                     /gene="soxA"
                     /locus_tag="PLH85_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HNB65643.1"
                     /translation="MRGFMRTALATAAVVLSAPVWAEGSTQDEIARYREMLAEGNPAE
                     LLEAKGEELWKKPRGPRNVSLERCDLGLGPGQVQGAYARLPRYFADTRQVMDVESRLV
                     HCMVTLQGFTREEATRNWYSRPGKDSDIEALVTYIGGQSNGVAIDVPARHPEEARMAR
                     IGELIFYRRSGPQDFSCAICHGQEGKRIRLQELGKLTTRAGAGTAMANWPSYRVSQGA
                     VWTMQRRLIDCMRQARWPEPEYLSDSVIALETFLQKTASGTVMQTPGIKR"
     gene            13912..14571
                     /gene="soxX"
                     /locus_tag="PLH85_13590"
     CDS             13912..14571
                     /gene="soxX"
                     /locus_tag="PLH85_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HNB65644.1"
                     /translation="MKTFAFTAGFAAILAGAVTAPHAQAADRTASADKTLAAQAEQVF
                     RSGFRRDNPPYWMARIEQDEAMRLCSQYKDDPPDAVRRRIEAAQRATLRYPVDGKLVG
                     DWREGEKLASSGRGGHIGFIQPDPPGTPRGGNCYACHELAKKEVAYGTIGPSLRHFGR
                     LRGSGDDVVRYAYEKIYNSNIFMACTNMPRFGLHSWLTPEQITHLVAFLIDPESPVNK
                     D"
     gene            14585..>15244
                     /locus_tag="PLH85_13595"
     CDS             14585..>15244
                     /locus_tag="PLH85_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNB65645.1"
                     /translation="MSMTRRDFLQVLAVAAAGGMALHSHPARAAKAAERLYDLPRFGS
                     VSLLHMTDCHAQLLPIHFREPDVNLGVGPMAGQVPHLVGEHLLRHFGIRPGTPEAHAF
                     SSLDFVKAARTYGKVGGFAHLATLVKRLKASRPGALLLDGGDTWQGSGLSLWTKAQDM
                     VEACKALGVDVMTLHWEATYGEARVKEIEEKDFAGHIDIVAQNIKTADFGDPVFKPYV
                     IR"
BASE COUNT         2464 a         5013 c         5310 g         2457 t
ORIGIN      
        1 ctcgtcgggc tcataacccg aaggtcgcag gttcaaatcc tgcccccgca accaataccg
       61 cagaacgccc agtcactcac gtggctgggc gtttttgcgt gtgcgctcag gctcgccgtg
      121 gctcggatgt ccgccgtgat gtggcgggca caaggctgcc ctgcatgatc cggtggccgg
      181 acagggggcc gtgtgttggt gcgcgccggg caggtgtccc gaagtgccac caataaatta
      241 gaatacaata atatattgac actttaagtg ttgtggcgta tcgtccgtct atcgatcccg
      301 tgctgttcat catgagcgat accgaaaccc tccccgatct ggaagcgatc cggcaggccg
      361 ccagtgcggc gaccgcggtg ctgagcgcgc tggccaatga aaaccggctg ctgctgctgt
      421 gccagctttc gcgtggcgag cgctcggtcg gcgagctgga ggccgagctg gatctgcatc
      481 agccaaccct gtcccagcag ttgagcgtcc tgcgcgccga aaacctggtg gcgacgcggc
      541 gcgatggcaa gcgagtcttc tattcgattg ccgacgtacg ggtgctggcg ctgctggaca
      601 ccctgtatga gcagttctgt ccccgaaagg acgtatgaac atcatgaaca tcgattggac
      661 ccatttcacg ccccaggcgg ccctcacggg gggcgcgctg atcggcctgg cgacgggcct
      721 gctgctggtg ctcaacggtc ggattgccgg catcagcggc atcctggggg ggctgctgca
      781 acgctgcagc gtgtctgaac tgagctggcg ggctgccttc gtggcggggc tggtggtggc
      841 gccagccctc ttcgggctgt tccttccctt gccggagatc cgtgtggacg cgcgctgggg
      901 cgggctggtc gtggccggtc tgctggtggg ttacggggcg cggctggggt ccggatgtac
      961 cagtggtcac ggggtgtgcg gactgtcgcg gatgtcgccg cgatcgctgg tggccaccct
     1021 gacgttcatg ggtacgggct ttatgaccgt ctttgtcgtc cgccatctgc tcgcttgagg
     1081 aggccgcaat gatcgtcttc actgcttttc tggcgggcct ggtgttcggc atcggcctga
     1141 tcgtgtcggg catggccaac ccgtccaagg tcacgggctt tctcgatctg gccggcgcgt
     1201 gggatccgtc cctggccttc gtgatggccg gtgccatcgc cctgggcgtc gtggggtacc
     1261 gggtcgcgct gcgacgcgga cgcccggtgt tcggggccgc gttgcagatt ccgacgctcc
     1321 ggaacatcga ccggcgtctg gtcgtcggga gtgcactctt cggcatcggc tgggggattg
     1381 cgggcatctg tccggggccc ggcctggtga tgctgggtgc cggtctcccg gaaggtcttg
     1441 tgtttgtttc ggccatgctc gtcggcatgg cgctcttcga gctccagcag ggtcatcttg
     1501 cgcgcctgct ggcagccctc ggcatcgcgc gcaactcctg aattacgcaa aggaaatcca
     1561 gatgtactcc gttcaagtgg atcgttcccc cgtcatcacc gtcaacggcg ccaagcacgc
     1621 ggtcagctac gcggtcgacg gctcgcagat gaatcccctc gaagcgttct acgccacgct
     1681 ggcaggctgc gctgcggtgt tcgcgaagaa ggcctgcaag gaactgggaa tttcgccgga
     1741 aggcatccac atcgcgtgca ggcccttcgc cggtgcggct ggccccttgt ccctgtcgaa
     1801 gttcaagacc gaggtgcgtt ttccggaggg tatagacgcc gcgcaaaaag cggcggtcct
     1861 cgaggcgatc aagcattgcg ccgtgaagca gatcgttgag gccggcgcag ccgtcgattt
     1921 ccaggtcgcc gaaggctgag gatcggtccg ccgccgcctg gcccgggaac gcgaacgacg
     1981 cggggcgcct tcaggccccg ccgggcggcg ggtctgcgac gtccatctcg cggatccgcc
     2041 ggtagagggt ccgctcgctg acgcccaggt gggtggcaag ggcccggcgg gtcccgcgga
     2101 agctgcgcaa cagggtggcc agcgttgcgt catccggcag cccgccagtg gtgacagcgt
     2161 atgaggcggt ggatgcgctc agggccgtgg ccggcagcag atcgtccggc aggtggcggc
     2221 gctccagggt gtctccatca cacagcagac tggcccgctc caggatgttg cgcagctcgc
     2281 gcacgttgcc ggggtagtcg taggccagca gatcatcgag ggcgtcgggt gcgacgccca
     2341 gtcggcgatc cggcgcaacc cggcgcagca gggcctcggc cagcagcggg atgtcctcgc
     2401 ggcgctcccg cagggccggc agggcgatgg ggaaggtgtt gatccggtag aacaggtctt
     2461 ggcggaagcg gccgtcgccg accaggcttt tcagcggccg gtgcgtcgcc gagatcaggc
     2521 ggatgtcggc gcgaaccagt tcggtgctgc cgacgcgccg gtaggtgccc gtttccagca
     2581 gacgcaacag cttgacctgc atgctcagcg ggatgtcgcc catttcgtcg aggaacaggg
     2641 tgccgccgct ggcggcctcc accagcccga ccttgcgtac cgtggcgcct gtgaaagcgc
     2701 cccgctcgtg gccgaacagt tcgctttcga acagggtctc ggtgagcccc gaacaatcca
     2761 ccgccacgaa gggccggtcg gcgcgccggc tggcgtcgtg aatggcgcgg gcgaccagtt
     2821 ccttgccggt gcccgattcg ccttgcagga gcacggtggc ttccgatgga gcgacccgcg
     2881 ccaccagttc aagcatgccc cggaaggtcg cagcgcgccc gatcaggcct tcgccgccgg
     2941 ccacgccccg cgccaccgga agcggctcca gtttttcgat gaagtaggcg atctcgccac
     3001 gggcgtcacg caccggtgaa agctcgatct tctcgtagcg ctcgccgctg gacgtatggt
     3061 gcagatgcac gacccgctca cgctggccgg actgaaggct gagggccagc gggcaggatt
     3121 cgccggcccg gtcgcagggc acggggtagc ggtgggacac ctcgtagcag gtgcgtccga
     3181 ccaggtcctc gggccgcgcg cacttggccc ggtaggccgc gttggccgcc aggatgcggt
     3241 aactgcggtc acacaggatg tggggctccg gcagggattc caggaaggac accagttccg
     3301 gcaggggatg ggcgggatgg gacatgggcg gaggcgggct gccaagggga ctgacacatg
     3361 ctgtcagggc cgcgggcggg cgtcaatccg tccgagcggg ggcgatcgga ccggggaggc
     3421 gtcgacgagg gcgtccgtgc actgtacggc acggcgcgcg ctgcttgggg tgcgctgcca
     3481 gatctgccga ttggcggaat gtggcagcgc gggcgtggca gttatggctg tgcagaagcg
     3541 cctcgtgcac ggcaagtgcc cgatttcaat gatgttttac aggccgaatg cttggcacgg
     3601 agcttgatag gtcgtctcca tcaccaatca agaacggctc ccgtgtcccg tgagccccct
     3661 gacggagaga cccgatgacc tgtgagacct gggcgcccgg cgcctgcgat cccgcccggc
     3721 gcagcctgct gcgggccact gccggagctg gcagcgtact ggctgtggcc accgccgttc
     3781 ccttcgtggc cagcctgacg ccttcccagc gggcccgcgc ggccggtgcg ccggtggagg
     3841 tggatatcga caagctggcg cccggcgagc tgatggtcgt cgaatggcgt ggcaaaccag
     3901 tgtggatcct gcgccggacg cccgagatgc tcgcctccct ggcccgcgcg gagcagcatc
     3961 tggccgaccc cgggtcggcc gtggacgccc agcagccaga ctatgcgcgc aacgcccatc
     4021 gttccatccg gcccgagatc tttgtcgccg tgggtctctg tacccatctg ggctgttcgc
     4081 cgtccagcgt gttcgagccc gggcatggcg cactgggcgc cgactggccc ggcggcttcc
     4141 tgtgcccctg ccatggttcc accttcgacc tggccggacg cgtgtatcgc aacaagccgg
     4201 cgccgaccaa tctggaggtg ccgccccaca ccttcctgtc ggatacccgc ctgcggatcg
     4261 gcgccgatgc cggcacgccc gcctgagcac ggagcccgac atgtcgcgac gtgatgcaga
     4321 caccgggccg cacgtgccca gggatccaat ggcgcgggcg ctggccctcc tgacgggtgc
     4381 ctttgcggcc ttcctgctgc tgtgttttcc gcaggtcctg gtccggtccg acggtgtggc
     4441 ggatccatgg gctgccgtgt gcctcatcgg cgcaatgatc accgggctgg cgagtggtgt
     4501 cggaacgccc ttcgagcggc gctggacgcg cgccctgctg tcccccgagt cctgcctgct
     4561 ggccctgacc ctggccaccc tgagggtggc cgcgcattga cggtgcaatc atgtacttcc
     4621 ggcaattctt cgatcccgcc acgtcggcca tgagctatct gctggccgat ccggcggccc
     4681 gcgcggcggc gattctcgat cccctcggtg atccgtgtca ggcgctggtc tttcgcgcac
     4741 tgctggcgga gcacgcgctg gacctgcacc tgatcctgct gacccatgtt cacggcaacg
     4801 ccctggagga catccggtcg acctatccgg acgtccggac agtggctggg gtcgggccgg
     4861 tgaccggcgt gtcggttgcg cgggagggcg acaggctgca cctcgggcgt gaggagatcc
     4921 gcatcatcgc cacgcccggc catacgttcc acagcgtgag ctatctgtgg cgtgaccgtc
     4981 tgttctgcgg agacgccctc gagctcggcg gttgcgccgc gtccgtcgag ggtgaaggcg
     5041 atgccggcca gcttttcgat tcggtgaccg ggcgactgtt cgccctggcg gacgagacgc
     5101 tggtgtttcc aggacacgat ttcaacggcc gcacggtgtc cacaattggc gaagagcgcc
     5161 gccgcaaccc ctttttccag tccctcaacc gagacgcctt caccgcggcc ctgcgcacgc
     5221 gggatgcggc cgatcggcag ttccggtggc cctgatcgtg gcgcggcgcg gcgcatcgaa
     5281 tgaaaggcgt cgaagggtga tgcgcgatgg cgcgtgcgga tgtcgcggtt gcgggcgccg
     5341 cgcggcgccc tgaatcggca tgggcgcggc tgtcggcgaa acgccccgga tgcgtccggg
     5401 tctgaaaggc gcgtggcgac atgaccatga tgtcgaaatg gtcaagtcct cggccgcctg
     5461 accgtaacaa caggtaccgc gcgacacagc gtggatggct gcaaggtttg accggggaga
     5521 aacatcatgt ctgtacgctt tcattccttg aaggcgcgtc tggcgctgct cggcctcatc
     5581 agcgtgctgg gggtctgcct gctggcggcg ctgtcattga tccagacgac gcggatcaag
     5641 gcgaatctgg tggtgttcgc cgatcgccac atcgccctca atcaccatgc gacccgcacc
     5701 tatgcccagg ggctgcagat ggggcaggcc ctgcgcaaca tcgtcctgga tccggcaaac
     5761 cgcaaggcct acgaaaattt cgcgcaggcg tccgagagct tcaaggaggt ctttggcgcc
     5821 ctggagcccc tcgtcaaggc ggaggatgcc gccgctgcgg aagaactcgc gcagcgcgtc
     5881 gcggcctggc gccccctcca ggcgcaggtg ctggccctcg tgcagggcgg agaggcggac
     5941 gccgccaggg cgctgctcac gacccgcgag acgccggcct ggcgcgccgt ccgggagatc
     6001 ctgctggcgc aggtgaagcg cagtgacgca gccgcggagg gcgcgcgggt gtccttgctc
     6061 gccgacgtgg acacgatcgc cgtcggggcg gcggtggtgg gtgttgcgac cctggccgtc
     6121 gtgctgggcg caaccctgtg ggtcggacgc gcggtgttcc gtcaggtcgg cggcgagcct
     6181 tctgccacgg cggcgtccct ggcacgtatt gcggccggag atctgaccgg ggagatccgt
     6241 ccgcgggcgg gagattgcga cagcatcctg gcggccgcga gcgacatgca ggcgcatctg
     6301 gccgggctgg tgcgcagcat cggccgcggc gcggtctcca tgatgcaggc cagtgaggca
     6361 atcgcgcgca atgccgagag cgtggccgac accgccgaac ggcagagtca ggccacgtcg
     6421 ggcattgcgg cggcggtgga ggaactgacc gtcagcatcg gcgtcatgtc cgacaacgcg
     6481 gtccacgcgg cctccctctc gggccagacg gagggcaagg tgcaggatgg actgacccag
     6541 gtgtcggcca cgacggacac catccgtcag gtggctgagg gtatgacggc ctttgccgaa
     6601 acgatgggcg agctgtcgtc caaggtgagc ggcatcaacg gcatcgtcca ggcgatccac
     6661 gagatcgccg agcagaccaa cctgctggcc ctcaacgccg ccatcgaggc ggcccgggca
     6721 ggcgagcagg ggcgcggatt tgcggtcgtg gcggacgagg tgcgcaaact cgccgagcgc
     6781 accgccactt cgacccagga gatctccgcg atgatcgcgg gcgtgcagga cagtacccgc
     6841 gtcgccctcg acaacatggc gcgcacccga gcggtggccc tggagggcgc gcgccacatg
     6901 gaccagaccc aggaggcggt ggccggtctg tcgaccgtgg ccgcgggtgt gcgtgaggag
     6961 gtcgatgcga tccgcgctgc cctggacgag cagcgcgcgg ccagcaccga catcgcgcgc
     7021 cgtgtcgagc agatcgccca ggggatcgag ggcacccacg tgtcggccgc agcatccagt
     7081 cgaagtgcgc aggaactcgt cgccctggcc cacgaactgg aggcaatgac cgggcggttc
     7141 cgggtgagcg cctgagccgg gccgcgggag aggcctcgag gatcgacaga taatcaataa
     7201 attcttatat tctataattc tgcacaaaac caaaaggggg agatggacat gggacttcgt
     7261 gtgaggggcg ccgtgctggc ggcctgttgg gtcgtggcca ccagcgccgg ggctgaagac
     7321 gtaaagcggg ctgaagagat cgtcggcggg cgctgcttcc tgtgtcatgg catggaaggc
     7381 gaggcggcgt ctgccctgta tccgcgcctg gccagccagc atgcggaata catcgccaag
     7441 cagctggcgg acttcaagag cgggcggcgc aagagcgaca ccatggccgg catggtggcg
     7501 gacctgactc cggacgagat gaagagcctc ggcctctact ttcaggccaa gccggcccgt
     7561 catggagaag ccggcgatgc cgatctggct ggcgtggggc gctacatctt caccaagggc
     7621 aacccatact ccggcgtggc ggcctgcacc gcctgccacg ggggcaaggg gcacgggacg
     7681 ccgcaactgc cccggctggc gggccagcag gccctgtatc tggaaaacca gctcaagcag
     7741 ttcaacacgc gcgaacgcac caacgacaac gccgtgatgc attccatcgc ctcgaagctg
     7801 accgagctgg aactgaaggc cgtgtcggcc tacatctcga ccctggactg aggggcgccg
     7861 aaagcctggc ggccgtctgc ctgcggccgg cccgtgtccg ctgggggcgg gccggccggg
     7921 gcgggcgtca ggtcagcatg tccgcccaga tgttgcgggc ccaggaatgg gcgtacttgc
     7981 cttccagttc gttggcggcc gccgagacgc cgccggcacc gggcacgggc agcacgacct
     8041 tcttcgcggc gtcgtactgg tgcacggagc tcacatggat gacgttcttc ggatcgacga
     8101 agctgtagca ggtgttcatc accaccggcg tcgggttggg cggctggccg gccaagaggt
     8161 tgaggatcgc cgcggccgcg accttggcgt gctggttggc catgtggccg gacttgggca
     8221 tggtcggcgc cgggaagagg gcgtcaccga tcacgtgtac gccgggcgtg ccgaccgctt
     8281 cgaagctcag ccagtccacc tccacccagc ggttgttgat gagcctgacg ccggacttcg
     8341 ctgccacgtc gccggcgcgc tggggcggca ccacgttgag cacgtcggcc ttgaccttgt
     8401 cgaactccag caccgccgtg cggctggcgg catccacctc ccgcagctcg ctgttgggca
     8461 ggtactcaag gatgcccttg tatttctcga aggcctttgt gaacagggcc ttcttcgaga
     8521 cgatctcctc attggcgtca aggatcagca ccttggagcg gggcttgttc cgctgcagat
     8581 agctggccac caggcaggcg cgctcgtagg ggccgggcgg gcagcggtag ggcgccttgg
     8641 ggatcgacag ggcgtacacg ccgccgtcac gcatgtccgc gagctgctgg cgcagggcga
     8701 cagtctgggg gccggccttc caggcgtgca ggacggtgct gcgggcggcg tcgctctcca
     8761 ggcccggcac ctgatcgaac atgaactcga tgcccggcga gagcaccagc cggtcgtaac
     8821 gcaggctgcc gccggaggcc agacgcaccg tgcgggcggt ggggtcgatg gcggtgactt
     8881 cgtcctgcac cacctgaacg ccccaggtgt gcttcaggcc gtcgtagctc agcgtgatgt
     8941 cggccagggt tttctcgccg ccgatcacca ggttggacat ggggcaggag atgaactggg
     9001 ggttgcgctc gatgagggtc acggacacgc tccgctcgct ccacatgcgc aggtacttgg
     9061 ccagggtggc gccgccgtag ccgccgccca ccaccactac atggccgtcg gggcggctgc
     9121 ccagagcgca gccggagagc ccgggaaagg ccgccgcgct gccggcgagg gccgcgctgc
     9181 gcagaaaatc gcgtcgattc gtcatggtcc gtccccctta cttgcgggcg gcgaagtacg
     9241 ccgcgatcag gtcgagctgc gcgtcggtgt atcccttggc gatctggtgc atgatcgtcg
     9301 cgggcttggc gccggaacgg aaatcctgga gcttctgcaa cagcttggcc ttgtccacgc
     9361 cggccagcgc ctcgttgccg gcgccgggca ccgcccggcc gtcggtgccg tggcagttgg
     9421 cgcaggtggc ggccagattg cgggccagct ccggatcggg gcgatccgcc gccagcgccg
     9481 ctccggcggt gcctgccagg cacagcacgg cgctccattg aagcagtttc atgggtctct
     9541 cctcctgtgt tccgccggtc ggcggagatg gtgtggcgtc ctcctggtct ctctggatcg
     9601 gggcgctggc cggtggttcc ggctctgaat cttcgtgcac tgcacgaata ttagtaattg
     9661 acgatattag tgtatggtta tagattgatt cgtatcaagg ggcgctcgca gattttacgc
     9721 atgcgccaag acatgcccgc cccgagcccg atgacaaccg aagggggaga cacccagatg
     9781 gctgagcagc ccatccagcg gggacgcctg cgtcctgcgc ccgaacattt cctgaacgag
     9841 gccgacgtgc aggcggtcga ggcgggacgc cgcggcttcc tgcgcaaggc cttggtcacc
     9901 gcgtcggcgg ccatggcggc caccacggtg gcccgtgcgg aggacggcga tccggccatc
     9961 ctcacgctgc cgccgtggac caccagcctg ggccagccgg tggcggcgcg gccctacggc
    10021 ctgccgtccc agtacgaaaa aggtctgcaa cggcgggaat cgccggggct cacgcggatg
    10081 tcccaggtgt cggtggcctt cacgccgctg cagggcctgt tcggcatcgt taccccgtcg
    10141 ggcctgcatt tcgagcgcca tcaccagggc tggagcgaca tcgacccgtc gaagcaccgc
    10201 ctgatgatca acgccagtga cgacggcttc atccgggcgc cgcgcgtcta taccgtggac
    10261 gacatcctcc gcctgccgcc ggtgtcgcgc atgcacttca tcgaatgcgg tgccaacacc
    10321 ggcatggagt ggggcaacgt ggcggtgccc acggtgcagt acacccacgg catgctgtcc
    10381 tgttcagaat tcaccggcgt gcccctgcgc acgctgctgg acgactgcgg ggtgaactgg
    10441 aagaaggcgc gttacgtgct cgccgaaggc gccgacggtt cgtccatgac ccgcacgatc
    10501 ccgatcgagc tcgtggagtc cggcgaggtg ctggtggcct acggtcagaa cggcgagatg
    10561 ctgcgccccg agaacggcta cccgctgcgc ctggtggtac ccggcgtgca gggggtgagc
    10621 tgggtcaaat ggctgcgccg catcgaggtg ggcgaccagc cctgggcgac caaggacgag
    10681 gccatccact acgtggacct gctgcccggc ggccagcatc gccaatacac ctccatccag
    10741 gaatgcaagt cggtcatcac gaccccgtcc gggggccaga tgctgctgga caagggcttc
    10801 tacaacatct ccgggctggc ctggtccggc cgcggcaaga tcacccgggt ggatgtgtcc
    10861 accgatggcg gcgtcaattg gcggtcggcg cgcatcgacg ggcccgtgca cggcaagtgc
    10921 ctgacccgct tcaatatcga ctgggtgtgg gacgggcgac cggcgatcct ccagtcccgc
    10981 gcggtggacg acaccggcta tgtgcagccc cgctacgggc agctgcgcgc cgtgcgcggg
    11041 accaaatcga tctatcacaa caacgcgatc cagtcgtgga aggtggcgga aacgggggag
    11101 gtgagcaatg tccaggttct ctaggattcc actggcgctg gttgccctgg tcgccctggg
    11161 gctggcgggg acggccgtgg cggacgggaa gttcccgggc attggtcggc cggccacggc
    11221 ggccgaggtg cgcgcctggg acatcgacgt gcgtcccgat ttcaagggcc tgcgccccgg
    11281 cagcggcagc gtggaccagg ggcaggaggt ctgggaggcc aaatgcgcgt cctgtcatgg
    11341 caccttcgga gagtccaacg aggtgcatac gccgctcatc ggcggcacca cacaggacga
    11401 cattcggacc ggtcacgtgg cttccctggc gaagggtgac gtgccccagc gcaccgccat
    11461 catgaaggtg gcgacggtgt ccaccctgtt tgactacatc aaccgggcga tgcccctgac
    11521 ggcccccaag agcctcaaga atgacgaggt ctatgccctg ctcgcctatc tgctcaatct
    11581 ggccgaggtg gtgcccgccg acttcgtgct gagcaactgg aacatcgccg aggtgcagca
    11641 gcgcatgccc aaccgcaagg gcatgaccac cgatcacggg atgtggcccg gtgcctcggc
    11701 ggctagggga ggcatcggca acggcggcac gccggacgtg aagaacaccg cctgcatgca
    11761 tcgctgcaag gacgcggtgc aggtggcgtc cctgctgccg gcctatgccg agacggccca
    11821 cggcaatctg gcggagcaga atcgcagcgt cggcccagtg cgtggcaagg agactggcgc
    11881 gcgggccatg gcggacgcga aatctgtggc gctgagcgag aatgcggcga tggcgaaact
    11941 cgccggtgac agcggctgca tggcctgcca cggtctggcc agccgcgtcg tcgggcccgg
    12001 ttacaacgaa gtggccaccc gctacacggg ccagcccgac gccgaggcga agctggcggc
    12061 caaggtcaag gccggcgggc agggtgtctg gggcgcgatc cccatgccgc cccagagcca
    12121 gttgaaggac ggggatgtgc gcagcctcgt gaagtggatc ctgtccggcg ccaaggcgca
    12181 ataaacgaca tcaacgaagg agagagagac ctatgaacat ggaacgccgt gaagtcctca
    12241 aggcgggcag cgggcttggc ctgctcggcc tgctggtcgc cgccggactg atcaccccgg
    12301 gcgaggcccg cgccgaatgg gccaaggcgg ccttcgacgc gaagagcatc gaagacaccc
    12361 tcaaggccct gggcgccggc aagccggaca gctcggccga catccagatc accgcgccgg
    12421 acatcgccga gaacggcgcc gtggtgccgg tgggcgtggt caccgcgctg cccgacgtgc
    12481 aggtcatcgc cttgctggtg gagaagaatc ccaatccgct ggcggccagc ttcacgctgc
    12541 cggccggcac cgagccgagc gtgcagaccc gcgtcaagat gggacagacc tccgacgtct
    12601 acgccgtggt caaggccggc ggcaagttcc tcatgaccaa gaaggaaatc aaggtcaccc
    12661 tcggcggctg cggcggctga tcaaacattc caggagattc ccatggcaga cccgatgaag
    12721 attcgcgcca gcgccaagga cggcatgacc gaagtgaagg tgctgatgag ccacgagatg
    12781 gagaccggcc agcgcaagga cgcctccggc gccctggtgc ccgctcattt catcaccgaa
    12841 gtgaccgccg agcacaacgg ccggccggtg ctggcggccc agttcggcac cgccgtgtcg
    12901 aagaacccct acctcgcctt caagttcaag ggcggggcca agggcgacaa gctgacagtg
    12961 agctgggtgg atagcaaggg cgacacgcgt cgcgacgaag tcctgatcgc ctgacgcgcc
    13021 ggggcgggcg catcccgccc atgccacaag accgccggtc aggcggcacc cccacacacg
    13081 aggaggaaga gacgatgcgt ggattcatgc ggacggcgct cgccaccgcg gccgtggtcc
    13141 tgagtgcgcc ggtctgggcc gagggctcca cccaggacga gatcgcccgt taccgggaaa
    13201 tgctcgccga gggcaacccg gccgagctgc tggaagccaa gggcgaggaa ctctggaaga
    13261 aaccccgcgg cccccggaac gtcagtctgg aacgctgcga cctgggcctc ggaccggggc
    13321 aggtccaggg cgcttacgcc cgcctgccgc gctacttcgc ggacacccgt caggtcatgg
    13381 acgtggagtc gcgtctggtt cactgcatgg tgacgctgca gggcttcacc cgcgaggagg
    13441 cgacgcgcaa ctggtattcg cggcccggca aggattcgga catcgaggct ctggtgacct
    13501 acatcggcgg ccagtccaac ggcgtcgcca tcgacgtgcc ggcgcgccac ccggaagagg
    13561 cccgcatggc caggatcggc gagctgatct tctatcgccg cagcggcccg caggacttct
    13621 cctgcgccat ctgccacggc caggaaggca agcgcatccg cctgcaggaa ctgggcaagc
    13681 tcaccacccg cgcgggagcc ggcacggcca tggccaactg gccgtcctac cgggtctccc
    13741 agggcgctgt ctggaccatg cagcgccgcc tcatcgactg catgcgtcag gcccgctggc
    13801 ccgagccgga atatctgtcc gactccgtca tcgccctgga gaccttcctg cagaagaccg
    13861 ccagcggcac cgtgatgcag acccccggaa tcaagcgctg aggccccgac catgaagacc
    13921 ttcgcattca ccgccgggtt cgccgcgatc ctggcgggcg ctgtcactgc gccccatgcc
    13981 caggcggccg accggaccgc gtcagccgac aagaccctcg ccgcccaggc cgagcaggtc
    14041 tttcgcagtg gcttccggcg cgacaatccg ccctactgga tggcccgcat cgaacaggac
    14101 gaggccatgc gcctgtgcag ccagtacaag gacgacccac ccgacgccgt gcgccgccgc
    14161 atcgaagccg cccaacgcgc caccctgcgc tacccggtgg acggcaagct cgtcggcgac
    14221 tggcgcgagg gcgagaagct cgcctccagc ggtcgtggcg gccacatcgg cttcatccag
    14281 cccgatccgc cgggcacgcc gcggggcggc aactgctatg cctgccacga gctggccaag
    14341 aaggaagtcg cctacggcac catcggcccc agcctgcgcc atttcgggcg cctgcgcggc
    14401 agcggtgacg atgtggtgcg ctatgcctac gagaagatct acaactccaa catcttcatg
    14461 gcgtgcacca acatgccccg tttcggcctg cacagctggc tcacgccgga gcagatcacc
    14521 cacctcgtcg catttctgat cgatccggaa tctccggtga acaaggactg acaggagacc
    14581 gcccatgtcc atgacccgtc gggattttct ccaggtgctc gctgtcgccg ccgcgggcgg
    14641 gatggccttg cacagtcatc ccgcccgcgc ggcgaaggcc gccgagcgcc tgtacgatct
    14701 gccccgcttc ggcagtgtca gcctgctgca catgacggac tgccatgccc agctgctgcc
    14761 catccacttt cgcgagcccg acgtaaacct gggcgtcggc cccatggccg ggcaggtgcc
    14821 gcacctggtg ggcgaacacc tgctgcgcca tttcggcatc cgccccggca cgccggaggc
    14881 ccacgccttc agttcgcttg acttcgtgaa ggccgcccgc acctacggca aggtcggcgg
    14941 tttcgcccat ctcgccaccc tcgtcaagcg cctcaaggcg agccggcccg gcgcgctgct
    15001 gctggatggg ggagacacct ggcagggctc cggcctcagc ctgtggacga aggcccagga
    15061 catggtggag gcctgcaagg ccctcggtgt agacgtgatg accctgcact gggaagccac
    15121 ctacggtgaa gcccgcgtca aggagatcga ggaaaaggac ttcgccggcc acatcgacat
    15181 cgtggcccag aacatcaaga ccgccgactt cggcgacccg gtgttcaagc cctacgtgat
    15241 ccgc
//
LOCUS       DAOQMM010000094        14918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1610_length_14918_cov_45.971675, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000094 DAOQMM010000000
VERSION     DAOQMM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14918
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_14918_cov_45.971675"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLH85_13600"
     CDS             <1..489
                     /locus_tag="PLH85_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNB65646.1"
                     /translation="KMHSFYLRNMYLQNLLKEPGGIEIGGLPIDLGKIKVPTYFISTI
                     EDHIAPWKSTYSGARNFGGNVRFVLGGSGHIAGIVNPPAANKYGYWINTAAKMPATAD
                     DFFAGAEQHPGSWWTDWQAWLMDQDSSKVPARDPASGKLTVLEDAPGSYVKARIELKA
                     KA"
     gene            599..1105
                     /locus_tag="PLH85_13605"
     CDS             599..1105
                     /locus_tag="PLH85_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNB65647.1"
                     /translation="MSAHILPDPLPAGLPVVLDTNTVLALWMFEDPRLPCLRSAVESG
                     HLRPFTRQDCLDELQRVLAYAQFGQPPERQAALAATYATRCARIEPVAELEVVLPACL
                     DRDDQKFLETARDAPARLLVTRDKLLLKLARRAVIAARFAILTPERIEALLARQGMAP
                     SPAPQSLT"
     gene            1169..1987
                     /locus_tag="PLH85_13610"
     CDS             1169..1987
                     /locus_tag="PLH85_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB65648.1"
                     /translation="MPIDSSLDVSKLVYTYLIPLGWKIAGAVALWIIGGWVINVIGNL
                     SSRGMDRQKVEPTLSRYVEATLRVIMRIVLVIVILSVFGIETTSFAALLAAAGVAIGA
                     AWSGLLANFAAGAFLIILRPFKVGDFISGGGVTGTVREIGLFATTVDQPDNVRTTIGN
                     NKLFADNIVNYTSNPFRRVELTAQIAHSVDPFEAIELIRRRIAEIPNVLASPAPDVEI
                     LEFNAAGTKLAVRPHCHNDHYWQVYFDTNKALAEVGGAHGWPIPAPHQVLRQPS"
     gene            complement(2080..2910)
                     /locus_tag="PLH85_13615"
     CDS             complement(2080..2910)
                     /locus_tag="PLH85_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNB65649.1"
                     /translation="MRDAIVNPLQDALGICHRPAARDARIVCLVPSITELVWDLGLGA
                     QLVGRTGFCVRPREVLRAVAKVGGTKDVNLEKLRALAPSHVIVNVDENRRETVEALAG
                     FVPHVIVTHPCAPQDNFALYRLLGGIFGRVAEAEALCAGLADALAQAYAAAADLPRER
                     VLYLIWRDPWMTVSAGTYIAATLAAVGWDAVAVASPARYPAFDWAEPAVQGAARVLLS
                     SEPYRFQLKHEAEVTALSGRPARRLDGEWASWYGSRAIAGLRALVRLRRSLHEGVAGA
                     "
     gene            3055..4170
                     /gene="ald"
                     /locus_tag="PLH85_13620"
     CDS             3055..4170
                     /gene="ald"
                     /locus_tag="PLH85_13620"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNB65650.1"
                     /translation="MKIGIPRESKNHEYRVGLTPGAVREALAHGHDVIVETGAGHGIG
                     ASDSDYERAGARIALNVDEVFGEVEMIVKVKEPLAAERARLRPHHLLFTYLHLAPDPG
                     QAEDLLASGATAIAYETVTAPGGGLPLLAPMSEVAGRMSIQAGAAALEKSHGGRGVLL
                     GGVPGVEPGRVVVIGGGTVGNAAARTALGMGADVTVLDRSLAVLRHLDDVYRGHLKTI
                     HATRDAIERHVLQADLVIGAVLIPGAAAPKLVSRAMLNAMKPGAVIVDVAIDQGGCFE
                     TSRPTTHADPTYVVDGVVHYCVANMPGAVARTATYALNAATLPYVLALADKGWKQALK
                     DDVHLRAGLNVCAGLVTHPQVAEALGKKYVPAITLLG"
     gene            4257..4727
                     /locus_tag="PLH85_13625"
     CDS             4257..4727
                     /locus_tag="PLH85_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB65651.1"
                     /translation="MNPSPLYTVEQYAQEDSIGFLFHQAKLRLTQAMDERIGDLGITA
                     AQWVVLKQIALRSGETASALCKCSGCDTGSMTRMLDRLEEKGLIRRERSTADRRVVQL
                     HVTDAGQALLPAIVPRVVDVLNTAVVDFSREELDMLKGLMRRVVANLDARAPQD"
     gene            4782..6269
                     /locus_tag="PLH85_13630"
     CDS             4782..6269
                     /locus_tag="PLH85_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNB65652.1"
                     /translation="MTDTAQATTCIPEQPRAVARPLVLIMTLLLGACVSPAGLAPRGT
                     PTDPATLAATRSLADTPAGDWPATTWWTAFGDPQLDRLVAAALQGSPTLDLARARIDR
                     ARAQAGVAAAVSDVHVGANVDGAQQRLSEHYIFPSGLGGTYTSINRAALDFSLDLDFW
                     GRNRAALAAAVGEARATEADAAEARLVLASAVVRSYLQFDLLHRLRAIADATVTQREH
                     LQKLVHTRQQAGLDARAEVQQAIGSVSAAKAERSALDTQLEQVRHQLAALTGQGPDAA
                     LDFTPPQVHAARVALPEVLPADLLGRRPDVVAQRWRVEAARQGSAAARAEFYPNVNLS
                     AFVGVQSLGLHPLFESGSRIVGIGPAIHLPVFDSSRLRANLAVRHGEEDIAIAQYNAT
                     LVDALRDVADQLSVWRGAHAQARDQDAALARYEEAWRLATVRYRAGLTSYTAVLAAET
                     PLLAQRRLSAELHNRQLDAGAGLARALGGGLALVTPPTPTTSTLR"
     gene            6293..7546
                     /locus_tag="PLH85_13635"
     CDS             6293..7546
                     /locus_tag="PLH85_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB65653.1"
                     /translation="MTAPTPDSTPNTARRRSLLAIATACGLAGLAWGGWWFTHGRFLE
                     QTDDAYVAGNVIQITPQVAGTVIAIDADDTQTVRAGQTLVRLDPADARVALEQAEAQL
                     GQSVREVQALYAGGGALEAAVRQREADLARARDDLQRRQQVAGSGAVAEEEIEHARAA
                     LRAAETALQTAREQLVTNQVQTRGTQIATHPNVQRAAARVEESYLAYARSAVPAPVGG
                     LVARRSVQVGQRVAAGAPLMAVVPLDQVWVDANFKESQLHGMHAGQPVTLAADVYGSR
                     VTYHGHIAGLAAGTGAAFALLPAQNASGNWIKVVQRVPVRIALDPRELAAHPLRIGLS
                     MQVEVDLRDSTDSDAPLAQAVPASPVAATDVYAELGKAARARIQAIIGANSGGPAAPP
                     LSEPVSPGTAPAAAAQATAPRRTQA"
     gene            7551..9098
                     /locus_tag="PLH85_13640"
     CDS             7551..9098
                     /locus_tag="PLH85_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNB65654.1"
                     /translation="MAGPIALPPLEPGARRLATVALSLATFMNVLDTTIANVSLPNIA
                     GDLGVSPSQSTWVITSFGVANAIAVPLTGWLSQRFGQVRLFVTSVVLFVIASWLCGLA
                     HSLEMLIAARVLQGAVAGPMIPLSQALLLQSYPREKSGMALAMWSMTTLVAPVMGPIL
                     GGWLSDNLSWPWIFYINLPVGLGAAWMSWQLLRERETPTRKLPIDRVGLVLLVVWVGA
                     LQIMLDKGKELDWFESGEIVALGAVALVGFALFVAWELTEAHPVVDLTLFARPNFLFG
                     VIALAAGYGVFFGNVVVMPLWLQQFMGYTATWAGLVTAPIGLLALLLSPFIGRIMPRT
                     DPRRIATVAFLVFALVSFLRAGFSTDIAPGNVVLPQVIQGIAMACFFIPLVAITLSGL
                     PMERVPSASGVSNFVRITCGAFGASLMTTLWERRAALHHEQLAAQVNAFNPLATQATG
                     GLQALGGDPAMSLAVLERQVTVQAFSLAANDVFWLSGVLFLVLVGTIWLTRPIHGRPA
                     PSGDAAH"
     gene            complement(9117..9884)
                     /locus_tag="PLH85_13645"
     CDS             complement(9117..9884)
                     /locus_tag="PLH85_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB65655.1"
                     /translation="MTLRVFITGASSGIGAALARRYAAQGAVLGLLARRGEALQALAA
                     ELGGTVACYVADVAEAGAVARAAADFIARHGVPDVVIANAGVSVGTLTEEAGDLAAFE
                     RVLRTNVLGLVATFQPFVAPMRARGSGRLVGIASVAGIRGLPGAAAYSASKAAAIACL
                     ESLRVELRGSGVKVVTIAPGYIRTPMTAVNTYPMPFLMDVDAAARRFVQAIAAGTSYT
                     VIPWQMGVVAKLLRLLPNPVFDALFARAGRKPRGLDL"
     gene            complement(9948..10592)
                     /locus_tag="PLH85_13650"
     CDS             complement(9948..10592)
                     /locus_tag="PLH85_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNB65656.1"
                     /translation="MQRRDVLKQLSAVVALAGTGLPVFAQGAGKGFELVSPPQPTDDK
                     NKVEVIEFFHYGCPHCKAFDPLLDLWIKKLPADVVFRRVPVTWGNPMLTGLARLHLAL
                     DVSGELPRLHGQVFDAVQTDKLPLNTEAGAIEWVTRKGGDGKKFADAYRSFGVQTRLA
                     RVEQLGRDYKIQGVPTLAIDGKYFTAASIAGSHEAALKVADQLIARARSEQGRK"
     gene            complement(10620..11381)
                     /locus_tag="PLH85_13655"
     CDS             complement(10620..11381)
                     /locus_tag="PLH85_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB65657.1"
                     /translation="MSRESTSRPKHVPTRPGSRGSTLVGIFVGLILGALVAAGIALYF
                     TSASPFQKQAKPDERAPAPAQPRPAPSGGEPIALPGKPGDKPVEKPRFDFYNVLPQGS
                     DALPAPSKPEETTEIVKPKPSDKSADKPAGSDDGDRAARILRGESADKTKAADAAAGD
                     KPVTPEKYYLQAGSFEDPSEADNLKARLALMGVEASVQKVDVPDKGTVHRVRVGPYLG
                     QEEMTKAKTQLASAGINAAVLRVKPKEPKPAGAAQ"
     gene            complement(11401..13134)
                     /gene="argS"
                     /locus_tag="PLH85_13660"
     CDS             complement(11401..13134)
                     /gene="argS"
                     /locus_tag="PLH85_13660"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB65658.1"
                     /translation="MSADPKNLLADLLRAALVSVAPEQAETPILLDRPKQAGHGDFAS
                     NLALQLAKPLKRNPRDLAALLVAELPASPLVARVEVAGAGFINFTLASDAKTRVVRQV
                     LEQGDAFGRGAKNGVKVQVEFVSANPTGPLHVGHGRGAAYGASLADVLAFAGFDVTRE
                     YYINDAGRQMDILALSTWLRYLDLFGIAIPFPPNAYQGDYVKDMAAQIREGHGDRFAK
                     VSAADVLQGAPAIDVDKETHLDVLIANAKRLLGSDYAWVHGFALTEQLGDGREDLEEF
                     GVTFDKWFSERSLFDTGLVEKAVAELDARGHLYEQDGARWFRSTAFGDEKDRVVQREN
                     GLFTYFASDIAYHANKYERGFDRIIDIWGADHHGYIPRVKGALQALGLPPEKLDVALV
                     QFAVLYRNGQKAAMSTRSGEFVTLRELRKEVGNDACRFFYVLRKADQHLDFDLDLAKS
                     QSNENPVYYIQYAHARVCSVLAQWNGVVADLAGADLARLDGERELALCARLAAFPEGV
                     QGAAADYAPHQIAFYLKDLAGDFHSYYNAERLLVDDEGLKLARLALAEAVRRVLASGL
                     ALLGVSAPQSM"
     gene            13298..13969
                     /locus_tag="PLH85_13665"
     CDS             13298..13969
                     /locus_tag="PLH85_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65659.1"
                     /translation="MRRRTRPFAPLLAAVAALAGGPPAHAEPPTLHWAVPLPAGQGWR
                     IVDLREGQGIRHEEYIPRGQGVDDYRDRILVQRQAAGEGMTPDTYLGYIGSGLAGHCS
                     GYTTSGLVPGQRDGLVTATRTAYCGRFDARPYGYVIAQKVIRDGEHLFIVEREWRLPP
                     FSVEPDGRTTLSPANPDEDAALKKDIRLAQRWLLEQVNPGAAPPPADPPSAPPPPVRK
                     AARKS"
     gene            14051..>14918
                     /locus_tag="PLH85_13670"
     CDS             14051..>14918
                     /locus_tag="PLH85_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNB65660.1"
                     /translation="MRLIRLVKIISVSLKYGLDQMILSNEPTGRLAWLARLARVRHFS
                     EPSGARLRMALESLGPIFVKFGQMLSTRRDLLPPAIAEELARLQDRVPPFPSGQAVAE
                     LEAAYGRPVDRVFARFDREPVASASIAQVHFAALPDGTEVAVKILRPGIAPVIAHDLA
                     LLDAFATLLEKVWPEGRRLKPREVVAEFSKYLRDELDLMREAANCSQLRRNFKDSELL
                     VVPEVYWDFCTANVMVMERMQGVPISQLDALRAHGIDLSALSRAGVEIFFTQVFRDGF
                     FHADMHPGNIFVA"
BASE COUNT         2267 a         5713 c         4740 g         2198 t
ORIGIN      
        1 aagatgcaca gcttctacct gcgcaacatg tacctgcaga acctcctcaa ggagccgggc
       61 ggcatcgaga tcggcggctt gcccatcgac ctgggcaaga tcaaggtccc gacctacttc
      121 atctccacca tcgaagacca catcgccccg tggaagagca cctactccgg cgcccgcaac
      181 tttggcggca acgtgcgctt cgtgctgggc ggttccggcc acatcgccgg catcgtcaat
      241 ccgccggcgg ccaacaagta cggctactgg atcaacacgg ccgccaagat gcccgccacc
      301 gccgacgact tcttcgccgg tgccgagcag catcccggct cctggtggac cgactggcag
      361 gcctggctga tggaccagga ctccagcaag gtgcccgccc gcgatcccgc cagcggcaag
      421 ctgacggtgc tcgaggacgc gcccggctcc tacgtcaagg cccgcatcga actgaaggcc
      481 aaggcctgat cggcatccgc cgtgcagctt gaggaagggg gcttcggccc ccttctgcat
      541 ttggacgccc gcccgccatt cattttcttt gcctcatcaa acctcctcgt accttgccgt
      601 gagcgcccac atcctgcccg acccgcttcc cgccggcctg cccgtcgtcc tcgacaccaa
      661 caccgtgctg gccctgtgga tgttcgagga cccgcgcctg ccctgcctgc gtagcgccgt
      721 cgaaagcggc cacctgcgtc cattcacccg ccaggactgc cttgacgaac tgcagcgcgt
      781 cctcgcctat gcccagttcg gccagccccc cgaacgtcag gccgccctcg ccgccaccta
      841 cgcaacccgc tgcgcgcgga tcgaaccggt cgcggagctc gaggtcgtcc tgcctgcctg
      901 cctcgaccgg gacgaccaga aattcctcga aaccgcccgc gacgcccccg ctcgcctcct
      961 cgtcacccgc gacaagctgc tcctcaagct cgcccgccgg gcggtgatcg ccgcgcgttt
     1021 cgccatcctc accccggaac ggatcgaggc cctgctggcc cgccagggga tggccccgtc
     1081 gccagcgcca caatctttaa catgacatac aattgctgac acgccggacc cgctagactc
     1141 cctccatccc cccaccagga gcaccgcaat gcccatcgat tccagtctgg atgtcagcaa
     1201 gctcgtctac acctacctca ttcccctcgg ctggaagatc gcaggcgccg tcgccctgtg
     1261 gatcatcggc ggctgggtga tcaatgtcat cggcaacctc tccagccgcg gcatggaccg
     1321 gcagaaggtc gagcccaccc tctcccgcta cgtggaagcc accctgcgcg tcatcatgcg
     1381 catcgtgctg gtgatcgtca tcctgtcggt cttcggcatc gagaccacca gcttcgccgc
     1441 cctgctggcc gcggccggcg tggccatcgg cgccgcctgg agcggcctgc tcgccaactt
     1501 cgccgccggc gccttcctga tcatcctgcg gcccttcaag gtgggtgatt tcatcagcgg
     1561 cggcggcgtc accggcaccg tgcgcgaaat cggcctgttc gccaccaccg tcgatcagcc
     1621 cgacaacgtg cgcaccacca tcggcaacaa caagctgttc gccgacaaca tcgtcaacta
     1681 cacctccaac cccttccgtc gcgtcgagct gaccgcccag atcgcccaca gcgtcgatcc
     1741 cttcgaggcc atcgaactga tccgccggcg catcgccgag atccccaacg tgttggccag
     1801 ccccgcgccg gacgtggaga tcctcgagtt caacgccgcc ggcaccaagc tcgccgtacg
     1861 cccccactgc cacaacgacc actactggca agtgtatttc gacaccaaca aggccctcgc
     1921 cgaagtcggc ggcgcccatg gctggccgat cccggccccg caccaggtgc tgcgtcagcc
     1981 gtcctgatcc ggtcgccccc ggcgcccccc tccggtcccc gtcccgcccc gtgcggggcg
     2041 gggatttttc atgggcgacg cagcgccatc agtccgtgct cacgcgcccg ccacgccctc
     2101 atgcagcgaa cgccgcagac gcaccagcgc ccgcaggccg gcaatggccc ggctgccgta
     2161 ccaggaggcc cactccccgt cgagccgccg ggccggccga ccggagaggg ccgtcacctc
     2221 ggcctcatgc ttcagctgaa agcggtaagg ctccgaggac agcagcacgc gggccgcgcc
     2281 ctgcacggcc ggctcagccc agtcaaacgc cggatagcgt gccggcgagg ccaccgccac
     2341 cgcatcccag cccaccgccg ccagggtggc agcaatgtag gtgcccgccg atacggtcat
     2401 ccacggatcg cgccagatca ggtacaggac ccgctcccgt ggcagatcgg ccgccgcggc
     2461 gtacgcctgt gccagtgcat cggcgaggcc ggcacacagg gcctcggcct ccgccacgcg
     2521 cccgaagatg ccgcccagga gccgatacag ggcaaagttg tcctgcggcg cacagggatg
     2581 ggtcacgatc acgtggggca cgaagcccgc cagggcctcc acggtctcgc ggcggttctc
     2641 gtccacattg acgatcacgt gggacggcgc cagcgcccgc agcttttcca ggttcacgtc
     2701 cttcgtcccg cccaccttgg ccaccgcacg caagacctcc cgcgggcgca cgcagaagcc
     2761 ggtacgcccc accagttgcg cgcccaggcc caggtcccac accagttcgg tgatggacgg
     2821 cacgaggcag acgatgcgcg catcccgggc ggcgggtcga tggcagatgc ccagggcatc
     2881 ctgtaacgga ttcacgatgg catccctcat gcttgctcac tcattaaagc ggcgcccctg
     2941 tcgtaaacgg aactggcagg gtaacagacc gctcagaccc tggagcgacc gggcatgcct
     3001 cgtgcaggcc gcgggtcctg cttctcgcgc aatcgtccca acacggagcc tcccatgaag
     3061 atcggcattc cccgcgaatc caagaaccac gaataccggg tcggcctgac accgggcgcc
     3121 gtccgcgaag ccctcgccca tggccatgac gtcatcgtcg aaaccggcgc cggacatggc
     3181 atcggcgcca gcgacagcga ctacgaacgg gccggcgcgc gcatcgccct caacgtggac
     3241 gaagtcttcg gtgaggtgga gatgatcgtc aaggtcaagg aacccctcgc cgccgagcgc
     3301 gcccgcctgc gcccgcacca tctgttgttc acctacctgc acctggcgcc tgaccccggc
     3361 caggccgaag acctcctcgc cagcggcgcc accgccatcg cctacgagac cgtcaccgcc
     3421 cctggcggcg gcctgcccct cctcgccccc atgtccgagg tcgccgggcg catgagcatc
     3481 caggccggcg ctgccgccct cgagaaatcc cacggcggac gcggcgtgct gctgggcggc
     3541 gtaccgggcg tggagccggg ccgggtggtg gtcatcggcg gcggcaccgt tggcaacgcg
     3601 gccgcccgca ccgccctcgg catgggcgcc gacgtcaccg tgctggaccg ctccctggcc
     3661 gtgctgcgtc acctggacga cgtctaccgg ggccacctga aaaccatcca cgccacccgc
     3721 gacgccatcg agcggcacgt cctgcaggcc gacctggtca tcggcgcggt gctcatcccc
     3781 ggcgccgcgg cccccaagct ggtgagccgg gcgatgctca atgccatgaa gcccggtgcg
     3841 gtcatcgtgg atgtggccat cgaccagggc ggctgcttcg agacctcccg ccccaccacc
     3901 cacgccgacc ccacctacgt ggtggacggc gtcgtgcact attgcgtcgc caacatgcca
     3961 ggcgccgtgg cgcgcaccgc cacctacgcc ctcaacgccg ccaccctgcc ctacgtgctg
     4021 gccctggccg acaagggctg gaagcaggcc ctcaaggacg acgtccacct gcgcgccggc
     4081 ctcaacgtgt gcgccggact cgtcacccat ccccaggtgg ccgaggccct gggcaagaaa
     4141 tacgtgccgg ccatcacgct gttgggttga actgcggccg tccagcgaaa aaggctcttg
     4201 acagggcctt tttcctgcac gattatctgc ctatgcagtc attgccctga caaacgatga
     4261 atccctcgcc cctctacacc gtcgagcagt acgcccagga agacagcatc ggtttcctct
     4321 ttcatcaggc caagctacgc ctcacccagg ccatggacga acgcatcggc gatctgggca
     4381 tcacggccgc ccaatgggtc gtcctcaagc agatcgccct gcgcagcggg gagaccgcct
     4441 cggccctgtg caaatgcagc ggctgcgaca ccggctcgat gacgcgcatg ctcgaccgcc
     4501 tcgaagaaaa aggtctgatc cgccgcgaac gcagcaccgc cgaccggcgc gtcgtgcagc
     4561 tgcacgtcac cgacgccggc caggccctgc tgccagccat cgtgccgcgc gtcgtggacg
     4621 tgctcaacac ggcggtcgtc gatttctccc gcgaagaact ggacatgctc aagggcctca
     4681 tgcgccgcgt cgtcgccaac ctggacgccc gcgctccgca ggactgagcg cccgccttcg
     4741 caacgggccc atgggcccat ttttgtgaac atataactgc catgacagat acagcacagg
     4801 caacaacatg catcccggaa cagccccgcg ccgtggcccg gcccctggtc ctgatcatga
     4861 cgctgctcct cggcgcctgc gtgagtcccg ccgggctggc accccgcggc acaccgaccg
     4921 atccggccac ccttgccgcg acccgcagcc tcgccgacac cccggccggg gactggcccg
     4981 cgacgacctg gtggaccgcc ttcggcgacc cgcagctcga ccgtctggtc gcggccgccc
     5041 tgcagggcag cccgaccctc gacctcgctc gcgcccgcat cgatcgggcg cgcgcccagg
     5101 ccggcgtcgc ggcggccgtc agcgatgtgc atgtgggcgc caacgtggac ggcgcccagc
     5161 aacgcctgtc ggaacactac atcttcccgt ccggcctcgg cggcacgtac acgagcatca
     5221 accgggccgc cctcgacttc agccttgacc tggatttctg gggccgcaac cgggcagcgc
     5281 tggccgccgc ggtcggcgaa gcccgcgcca ccgaggccga cgccgccgag gcgcgcctcg
     5341 tgctggccag cgcagtggtg cgcagctacc tccagttcga cctgctgcac cgcctgcgcg
     5401 ccattgccga cgccaccgtc acccagcggg aacacctcca gaagctcgtg cacacgcgcc
     5461 agcaagccgg cctcgacgcc cgcgccgaag tccagcaggc gatcggcagc gtgtccgccg
     5521 ccaaggccga acgcagcgcc ctcgacaccc agctggagca ggtccgtcac cagctcgccg
     5581 cgctcaccgg ccagggcccg gacgctgccc tggacttcac cccgccccag gtgcacgccg
     5641 cccgcgtcgc cctgccggag gtgctgcccg ccgacctcct cggccgccgc ccggacgtgg
     5701 tcgcccagcg ctggcgcgtc gaagccgccc gccagggcag cgccgccgcc cgcgccgagt
     5761 tctaccccaa cgtcaacctg agcgccttcg tcggcgtaca gtccctcggc ctccatcccc
     5821 tcttcgaaag cggcagccgc atcgtcggca tcggcccggc gatccacctg ccggtgttcg
     5881 acagcagccg cctgcgcgcc aacctcgccg tacgtcacgg cgaagaggac atcgccatcg
     5941 cccagtacaa cgccaccctg gtggacgccc tgcgcgacgt ggccgaccag ctcagcgtct
     6001 ggcggggcgc ccacgcgcag gcgcgcgacc aggatgccgc cctggcccgc tacgaggagg
     6061 cctggcgcct tgccaccgtg cgctaccgcg ccggcctcac gtcctacacc gccgtcctcg
     6121 ctgccgagac gccgctactc gcccaaaggc gcctcagcgc cgaactccac aaccgccagc
     6181 tcgacgccgg cgccggcctc gcccgtgccc tgggcggcgg gctggccctc gtcacgcccc
     6241 ccaccccgac gacctccacc ctccgctgat cccgccaaga aggaaccgga ccatgaccgc
     6301 ccccaccccc gacagcacgc ccaacaccgc ccgccgccgc agcctcctcg ccatcgccac
     6361 cgcctgcggg ctggccggcc tggcctgggg cggctggtgg ttcacccacg gccgcttcct
     6421 ggagcagacc gacgacgcct acgtggccgg caatgtcatc cagatcactc cgcaagtggc
     6481 cggcaccgtc atcgccatcg acgccgacga cacccagacc gtgcgcgccg gccagaccct
     6541 ggtccgcctc gacccggccg acgcccgcgt cgccctggaa caggccgagg cccagctcgg
     6601 ccagagcgtg cgcgaggtgc aggccctcta cgccggcggc ggggccctcg aagccgccgt
     6661 ccgtcagcgc gaggccgatc tcgcccgggc ccgcgacgat ctgcagcgcc gccagcaggt
     6721 ggccggcagc ggcgccgtgg ccgaggaaga gatcgagcat gcccgcgccg ccctgcgcgc
     6781 cgccgaaaca gccctgcaga ccgcccgcga gcagctcgtc accaaccagg tgcagacccg
     6841 cggcacccag atcgccaccc accccaacgt gcagcgcgcc gccgcccgcg tggaggagag
     6901 ctacctcgcc tacgcccgca gcgccgtgcc ggcgccggtg ggtggactgg tggcacggcg
     6961 ctcggtgcag gtcggtcagc gcgtggccgc cggcgcgccg ctgatggccg tcgtacccct
     7021 cgatcaggtg tgggtggacg ccaacttcaa ggaaagccag ctccacggca tgcacgccgg
     7081 ccagccggtg accctcgccg ccgacgtcta tggcagccgc gtgacctacc acggccacat
     7141 cgccggcctc gccgccggca ccggcgccgc tttcgccctg ctgccggccc agaacgccag
     7201 cggcaattgg atcaaggtgg tgcagcgggt ccccgtgcgc atcgccctcg acccccggga
     7261 gctggccgcc cacccgctgc gcatcggcct gtccatgcag gtcgaggtgg atctgcgcga
     7321 cagcacggac agcgacgccc ccctcgccca ggccgtgccc gccagcccgg tggccgctac
     7381 cgatgtctat gccgagctcg gcaaggctgc gcgggcgcgc atccaggcca tcatcggcgc
     7441 caacagcggc ggtcccgcgg cgcccccgct ctccgagccg gtgtcgcccg gcacggcgcc
     7501 ggcagccgcc gcccaggcca ccgccccgcg gcgcacccag gcctgacatc atggccggcc
     7561 ccatcgccct gccgcccctc gaaccggggg cgcgccgcct cgccaccgtc gccctgtccc
     7621 tggcgacctt catgaacgtg ctggacacga ccatcgccaa cgtgtccctg cccaacatcg
     7681 cgggggacct gggggtgagc ccgagccaga gcacctgggt catcacctcc ttcggcgtgg
     7741 ccaatgccat cgccgtgccc ctcaccggct ggctcagcca gcgcttcggg caggtgcgcc
     7801 tgttcgtcac ctcggtggtg ctgttcgtca tcgcctcgtg gctgtgcggt ctggcccaca
     7861 gcctggagat gctcatcgcc gcccgtgtcc tgcagggggc cgtggcgggc cccatgatcc
     7921 ccctctccca ggccctgctg ctgcagagtt acccgcggga aaagtccggc atggccctcg
     7981 ccatgtggtc catgacgacc ctggtggccc cggtgatggg ccccatcctg ggcggctggc
     8041 tgtcggacaa cctgtcctgg ccgtggatct tctacatcaa cctgccggtt ggcctcggcg
     8101 ccgcgtggat gagctggcag ctcctgcgcg agcgggagac gcccacccgc aagctgccca
     8161 tcgaccgggt cgggctcgtc ctgctcgtgg tgtgggtcgg cgccctgcag atcatgctcg
     8221 acaagggcaa ggagctggac tggttcgaat ccggcgagat cgtcgccctc ggcgccgtcg
     8281 ccctggtggg cttcgccctg ttcgtggcct gggagctgac cgaggcccac ccggtggtgg
     8341 acctcaccct cttcgcacga cccaacttcc tgttcggcgt gatcgccctg gccgccggct
     8401 acggcgtgtt cttcggcaac gtggtggtga tgcccctgtg gctgcagcag ttcatgggct
     8461 acaccgccac ctgggcgggt ctcgttaccg cgcccattgg tcttctcgcg ctgctcctgt
     8521 cacccttcat cgggcgcatc atgccgcgca ccgatccgcg ccgcatcgcc accgtggcct
     8581 tcctggtgtt cgccctggtg tccttcctgc gcgcgggatt ttccaccgac atcgcgccgg
     8641 gcaacgtggt gctgccccag gtgatccagg gcatcgccat ggcctgcttc ttcattccgc
     8701 tggtggccat caccctgtcg ggcctgccga tggagcgggt gccctccgcc tcgggcgtgt
     8761 cgaacttcgt gcgcatcacc tgcggggcct tcggcgcctc gctcatgacc accctgtggg
     8821 agcgccgcgc cgcgctgcat cacgaacagc tggcggccca ggtgaatgcc ttcaaccccc
     8881 tcgccaccca ggccaccggc ggcctccagg cgctgggcgg cgacccggca atgagcctcg
     8941 cggtgctgga gcggcaggtc accgtgcagg ccttctccct cgccgcgaac gacgtgttct
     9001 ggctgtctgg cgtgctgttc ctggtcctgg tcggaacgat ctggctgacc cgaccgatcc
     9061 acggccgtcc cgccccgtcc ggagacgccg cccactgacc cgcggcgccg cgcaggctac
     9121 aggtcgagcc cgcgcggctt gcgcccggca cgggcgaaca gggcgtcaaa gacgggattg
     9181 ggcagcaggc gcagtagctt ggccacaacg cccatctgcc acgggatcac ggtgtaggag
     9241 gtgcccgccg cgatggcctg cacgaagcgg cgggccgccg cgtccacgtc catcaggaag
     9301 ggcatcggat aggtgttcac cgcggtcatc ggcgtgcgga tgtagcccgg cgcaatcgtc
     9361 accaccttca cgccgctgcc gcgcagctcc acccgcaggc tctccaggca ggcgatggcc
     9421 gccgccttgg acgcgctgta ggccgcggcg ccgggcaggc cgcggatgcc cgccacggac
     9481 gcgatgccca ccaggcgacc gctgccccgc gcccgcatgg gcgccacgaa gggctggaag
     9541 gtcgccacca ggcccagcac attggtccgc agcacccgct cgaaggcggc cagatcgccg
     9601 gcctcctcgg tgagagtccc gaccgacacg ccggcattgg cgatcaccac gtccggcacg
     9661 ccgtgccggg cgatgaaatc cgccgccgcg cgagccacgg cgcccgcctc cgccacatcc
     9721 gcgacataac aggccaccgt gccgcccaac tcagcagcca gggcctgcaa ggcctcgccg
     9781 cgccgcgcca gcaggcccag cacggcgccc tgggcggcat agcgacgagc cagggccgcg
     9841 ccgatgccgc tcgacgcacc ggtgatgaac acccgcaggg tcatggggaa acaggaaacg
     9901 tggggaatgt gcgcccaccc gacccaaggc cgggcaggca ggagaactta cttgcggccc
     9961 tgctcgctgc gggcgcgggc gatgagctgg tcggccacct tgagggccgc ctcgtgactg
    10021 ccggcgatgg acgccgcggt gaagtacttg ccgtcgatgg ccagggtcgg cacgccctgg
    10081 atcttgtagt cgcggccgag ctgttcaacc cgcgccaggc gggtctgcac gccgaaggag
    10141 cgataggcgt ccgcgaactt cttgccgtcg ccgcccttcc tggtcaccca ctcgatggcg
    10201 ccggcctcgg tgttgagggg cagcttgtcg gtctgcaccg cgtcgaagac ctggccgtgc
    10261 aggcgcggca gttcgccgga cacgtcgagg gccagatgca ggcgggccag cccggtcagc
    10321 atcgggttgc cccaggtcac gggcacgcgg cggaacacca cgtccgccgg cagcttcttg
    10381 atccacaggt ccagcagcgg atcgaaggcc ttgcagtggg ggcagccgta gtggaagaac
    10441 tcgatgactt ccaccttgtt cttgtcgtcc gtcggctggg gcggcgacac cagctcgaaa
    10501 cccttgcccg cgccctgggc gaacacgggc aggccggtgc cggcgagggc gacgacggcg
    10561 gaaagctgct tgagcacatc acgacgctgc attgcggaat ctcctggttc gcaaagacct
    10621 tattgagcag ccccggcggg ctttggttcc ttgggcttga cccgcagcac tgcggcattg
    10681 atgccggcgg aggccagctg ggtcttggcc ttggtcattt cttcctggcc cagatagggg
    10741 cccacgcgca cccgatgcac ggtgccctta tccggcacat ccaccttctg cacgctggct
    10801 tccacgccca tcagggcgag ccgcgccttg aggttgtcgg cctcggacgg gtcttcgaaa
    10861 gagccggcct ggaggtagta cttctccggc gtgaccggct tgtcgccggc ggcggcatcg
    10921 gccgccttgg tcttgtccgc cgactcgccc cgcaggatgc gcgcggcgcg gtcgccgtcg
    10981 tcgctgccgg cgggcttgtc cgccgacttg tcggatggct tgggcttgac gatctcggtg
    11041 gtctcttcgg gcttggacgg cgccggcagg gcatcggatc cctggggcag cacgttgtag
    11101 aagtcgaagc ggggcttttc caccggcttg tcgcccggct tgccgggcag ggcgatgggc
    11161 tcgccgccgg aaggcgccgg gcggggctgc gctggcgcgg gggcgcgctc gtccggcttg
    11221 gcctgcttct ggaagggcga ggccgaggtg aagtacaggg caatccccgc cgccaccagt
    11281 gcgcccagga tcaggccgac gaagatgccg accagggtgc tgccccggct gccaggccgg
    11341 gtcggcacat gcttcgggcg ggacgtggat tcgcggctca tggacaggat ttccggacac
    11401 tcacatggat tggggcgcgc tcacgccgag cagggccaga ccgctggcca gcacgcggcg
    11461 cacggcctcc gccagggcga ggcgggccag cttgaggccc tcgtcgtcca ccagcaggcg
    11521 ctcggcgttg taatagctgt ggaaatcgcc ggccaggtcc ttgaggtaga aggcgatctg
    11581 gtggggcgcg tagtccgccg cggcaccctg caccccctcc ggaaaggccg ccagacgggc
    11641 acacagggcc agctcccgct cgccatcgag gcgcgccaga tcggcgccgg ccagatcggc
    11701 gaccacgccg ttccactggg ccagcaccga gcacacgcgg gcgtgggcgt actggatgta
    11761 atacaccggg ttctcgttgc tctggctctt ggccagatcc aggtcgaaat ccagatgctg
    11821 gtcggccttg cgcagcacgt agaagaagcg gcacgcgtcg ttgcccactt ccttgcgcag
    11881 ctcacgcagg gtgacgaact cgccggagcg ggtggacatg gccgccttct ggccgttgcg
    11941 gtagagcacc gcgaactgca ccagggccac atcgagcttc tcgggcggca gacccagtgc
    12001 ctgcagcgcg cccttcaccc gcgggatgta gccgtggtgg tcagcgcccc agatgtcgat
    12061 gatgcggtcg aagccgcgct cgtacttgtt ggcgtggtag gcgatgtcgg aggcgaagta
    12121 ggtgaacagg ccgttctccc gctgcacgac gcggtccttc tcgtcgccga aggcggtgga
    12181 gcggaaccac ctggcgccgt cctgttcgta gaggtggccg cgggcgtcca gttcggccac
    12241 agccttctcg accagacccg tgtcaaacag ggagcgctcg gagaaccact tgtcgaaggt
    12301 gacgccgaac tcctccagat cctcgcggcc gtcaccgagc tgctcggtga gggcaaaacc
    12361 atgcacccac gcgtagtcgc tgcccagcag gcgcttggca ttggcgatca ggacgtccag
    12421 gtgggtttcc ttgtccacgt cgatggccgg cgcgccctgc agcacgtcgg cggcggagac
    12481 cttcgcgaag cggtcgccgt ggccttccct gatctgcgcc gccatgtcct tgacgtagtc
    12541 gccctggtag gcgttgggcg ggaagggaat ggcgataccg aacagatcca gataacgcag
    12601 ccaggtggac agggccagga tgtccatctg ccggccggcg tcgttgatgt agtactcgcg
    12661 ggtcacgtcg aagcccgcaa aggccagcac gtcggcgagg gacgcgccgt aggccgcgcc
    12721 gcggccgtgg cccacgtgga gcggcccggt ggggttggcg gagacgaact ccacctgtac
    12781 cttgacgccg ttcttggcgc cacgcccgaa ggcgtcgccc tgctccagca cctgacgcac
    12841 cacgcgggtc ttggcgtcgg aggcgagggt gaagttgatg aagccggcgc ccgccacctc
    12901 caccctggcc accagcggcg aggcgggcag ctcggccacc agcagggcgg ccaggtcacg
    12961 cggattgcgc ttgaggggct tggccagctg cagcgccagg ttgcttgcga aatccccgtg
    13021 cccggcctgc ttgggacggt cgaggaggat gggggtttcg gcctgttcgg gggccacgct
    13081 gacgagggcg gcgcggagca ggtcggcaag caggttcttc ggatcggcgc tcatggaaca
    13141 cacagcaact aaaagcgtga aattatagcg aagcgccgca cttcccgcct gttgctggtg
    13201 tgaccgatgt ccggcgcatt cccgactggc acacggagaa ttgttgtctt atgtttcagg
    13261 gattgcatgc aggtcccgct ccgacgacgc tgataccgtg agacgccgca cccgcccctt
    13321 cgccccgctg ctcgctgccg tcgccgccct cgccggtggc ccgcccgcgc acgccgagcc
    13381 gcccaccctg cactgggccg tgccgctgcc ggccggccag ggctggcgca tcgtcgatct
    13441 gcgcgaaggc cagggcatcc gccacgagga atacatcccc cgcggccagg gcgtggacga
    13501 ctaccgcgac cgcatcctgg tgcaacgcca ggccgccggc gaaggcatga cgcccgacac
    13561 ctacctgggc tacatcggct ccgggctggc cgggcactgc tcgggctaca ccaccagcgg
    13621 cctggtgccg ggccagcgcg acggcctcgt caccgccacc cgcaccgcct actgcggccg
    13681 cttcgacgcc cgcccctacg gctacgtcat cgcccagaag gtcatccgcg acggcgagca
    13741 cctcttcatc gtcgagcggg aatggcgcct gccgcccttc agcgtcgagc cggacggccg
    13801 caccaccctc agccccgcca acccggacga ggacgctgcc ctcaagaagg acatccgcct
    13861 cgcccagcgc tggctgctgg agcaggtcaa tcccggcgct gcgccgccgc cggccgatcc
    13921 cccgtccgcc ccgccgcccc ccgtccgcaa ggccgcccgc aaatcctgag gcccgccggg
    13981 cggtcggacc gccctatgcg cagcagggct ttacgggcac aatcgccgtt tttccgaccg
    14041 ccctcccgcc gtgcgcctca tccgactcgt caagatcatc tccgtcagcc tgaaatacgg
    14101 gctcgaccag atgatcctct ccaacgagcc gaccggccgc ctggcctggc tggcgcggct
    14161 ggcgcgggtc cgccacttca gcgaacccag cggcgcgcgc ctgcgcatgg cgctggaatc
    14221 cctcgggccg atcttcgtca agttcgggca gatgctgtcc acccggcgcg acctgctgcc
    14281 gccggccatc gccgaagaac tcgcgcgcct ccaggaccgg gtgccgccct tccccagcgg
    14341 gcaggccgtg gccgagctgg aggccgccta cggccgcccg gtggaccggg tcttcgcccg
    14401 cttcgaccgg gaacccgtcg cctccgcctc catcgcccag gtgcactttg ccgccctgcc
    14461 cgacggcacc gaggtggcgg tcaagatcct gcgccccggc atcgccccgg tcatcgccca
    14521 cgacctggcc ctcctcgacg ccttcgccac cctgctggag aaggtctggc ccgaaggccg
    14581 ccgcctcaag ccgcgggagg tggtggccga gttctccaaa tacctgcgcg acgagctcga
    14641 cctgatgcgc gaggccgcca actgttccca gctgcggcgc aacttcaagg attccgagct
    14701 cctcgtcgtg cccgaggtgt actgggattt ctgcaccgcc aacgtgatgg tgatggagcg
    14761 catgcagggc gtgcccatct cccagctcga cgccctgcgc gcccacggca tcgacctcag
    14821 cgccctgtcc cgcgccggtg tggagatctt cttcacccag gtcttccgcg acggcttctt
    14881 ccacgccgac atgcaccccg gcaacatctt cgtcgcca
//
LOCUS       DAOQMM010000095        14887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1618_length_14887_cov_36.173476, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000095 DAOQMM010000000
VERSION     DAOQMM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14887
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_14887_cov_36.173476"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..308
                     /locus_tag="PLH85_13675"
     CDS             96..308
                     /locus_tag="PLH85_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNB65661.1"
                     /translation="MATNLVVRNIDPALVQALKEAAAAHGRSAEAEHREILRASLARP
                     PRRSLKDVLADMPDVGLDDDFNLRHG"
     gene            308..718
                     /locus_tag="PLH85_13680"
     CDS             308..718
                     /locus_tag="PLH85_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB65662.1"
                     /translation="MYLLDTNFVSELRKGPRANPGALAFFATLRAEDLYLPVQTLGEI
                     RRGVENLRGRGDHPQAERLEAWLDRLIAEHAPRILDFDRDCAQIWGRLLSPHPQHPID
                     KQIAAIALLYDLTVVTRNSADFAPLGVRHHNPFS"
     gene            complement(1077..2213)
                     /locus_tag="PLH85_13685"
     CDS             complement(1077..2213)
                     /locus_tag="PLH85_13685"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNB65663.1"
                     /translation="MTVVRLGTVCSQVRESIKPGERAALRYVGMESIESGTGRFLEGG
                     LSKTPESPLANSFRFGPEHVLYGKLRPYLNKVALPDFEGKCSTEIVPLKVNATVDRRY
                     LAYFLRCPDVVRVISDKTAGSRMPRADMAFVLSLPFLLRDLSEQSRIVDLLTRAESIL
                     RLRREAEKKTAELVPALFLQMFGDPVTNPKGWRQHLFSEVGVLDRGRSRHRPRDAAEL
                     YGGPYPFIQTGDVSNSGGTISSCVNTYSEQGLSQSKLWAPGTLCITIAANIARTAVLN
                     ISACFPDSVVGFVAGDMVCTRYVQTWLDFLQPTLEMHAPQAAQKNINLEILRNLPISV
                     PPKNLQEAFVFRFETLRALQSQQAAATAQAQASFDALLARAFAA"
     gene            complement(2210..3862)
                     /locus_tag="PLH85_13690"
     CDS             complement(2210..3862)
                     /locus_tag="PLH85_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNB65664.1"
                     /translation="MPLTPDMRRSIDQIRDYLFGGGYPDPVSNAEQLAFLFFFYLVEG
                     IDTANGHKARVLKRPYQSLFAGEWDLRNPLNAPVEGQKSLPRAHFRWSVWAKGYSGEA
                     LVRFLRDEVFPFYAELGARSAHNFMDGARLSIDEPTVLTQVIALVDGLRLDQSDADTK
                     GDLFEHVLRQIKQAGELGQFRTPRHIIRAIVRLLDPQVGETVYDPAAGTAGFLVAAYD
                     HMRLAHSSAAAIHQDEVDGKPVRRGLGDRLSAAQVDALASATFYGNDVDPKMVRLATM
                     NLTLRGLPDVHILKRNVLTTTLDAAEKAERGLPADGFHVILANPPFSGRVDKDRIVED
                     VKVGSTTATELLFLKYMLDSLRAPSDPASGAGGGRCGVIVPEGVLFGSTGAHRELRRQ
                     LIEHNRVDAVLSLPGGVFQPYSGVKTSVLVFRRGGRTQDVLFLHAEQDGYKLDANHDT
                     PIDADDLPALLSAYAERATARTRWQRRPADADWTERWWFADVDTLRANDYNLSAGRYR
                     PQTRSTVEHRDPRLLLAELAAIEAEIVEEVEALRALLGEGGE"
     gene            complement(3890..6052)
                     /locus_tag="PLH85_13695"
     CDS             complement(3890..6052)
                     /locus_tag="PLH85_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNB65665.1"
                     /translation="MHDNEAHARVAINAQLADQGWDLHNPNSVCYEVALDDGIADYVL
                     NDRHGRALAVTEAKRFTVDPATARDQAGRYARQLGVAYVFLANGHEILFWDWQREAHP
                     RPVKTFFGQDDLERRAAVRQIRIPLDRVPIDTRIAGRDYQLACIDTLSAEIARGRRKL
                     LVEMATGTGKTRTAAALIKRLFEAHAITRVLFLVDRIPLATQAEDAFAEHLPDFPAYV
                     LRAGRRFQDEKRITVTTLQSMVNLYRDYSSGYFDLVVTDECHRSIYGKWSGVLRHFDG
                     LQIGLTATPCVAPPGDERAPDPDDEDRLFVRDTLRFFEVEQPTFSYGLRQAIADGHLV
                     PYQIYRARTVRTATEGGFTVTRGELDWNALDADTRTELTQLFGPDDTLTVDPNALERR
                     FTIPARNQAIVTEFRKVMDEGFRDHKGILRKPLLGKTIVFAVTKRHAATLAKLFDDAF
                     ADCKPDPAVAYADYVVSGCGDDDTTDGLTKIQRFKRQEFPRILVSVNMLDTGFDCADV
                     ANLVFARFTRSTILYQQMRGRGTRRAPNKPVFTLFDFVGVVDFHGDADTYGEGGLVRE
                     TPARRRYSPRRLLGLPIDDTIDPASREWITLDADGNLVFPAASERRAAQLGTRFEAWM
                     LHNADLSAEAERWLHLLGNQIRANADTLDEITPEHFAYFHVFAQMGGLAEARRVLGGD
                     ARLAALLGALNAAVFSPLDDASADPAPGPDDAPRPTAP"
     gene            complement(6140..6331)
                     /locus_tag="PLH85_13700"
     CDS             complement(6140..6331)
                     /locus_tag="PLH85_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65666.1"
                     /translation="MEDPVLYRFCVRAKASPDQPEASRPAFITPYPLTEADAAERFEV
                     LECLQHTAQPLSSVSYVFD"
     gene            6460..6810
                     /locus_tag="PLH85_13705"
     CDS             6460..6810
                     /locus_tag="PLH85_13705"
                     /inference="COORDINATES: protein motif:HMM:NF006749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HNB65667.1"
                     /translation="MLHYAVKVAVSAGLIVAISEIARRSSGVAALLAALPLTSVLAFV
                     WLYVEGQPTEAIATLSGQIFWLVLPSLVLFVALPWLLRQGVGFWAALALAMGLTAGAY
                     AVLRPLLRHLGVEL"
     gene            7063..7764
                     /locus_tag="PLH85_13710"
     CDS             7063..7764
                     /locus_tag="PLH85_13710"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF009732.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HNB65668.1"
                     /translation="MAHPDHLLWRQGWNANRIPFHLTHIHPLLRRFWDTLDPAATARV
                     FVPLCGKSLDLMWLHGLGHDVVGIELSAVAVGDFFKAARLRPQRTRQADLTCWTQERL
                     AIFCGDFFDLTEEDLQGVSAVYDRAALTALPEGLRADYVAHLHAILPAGCQVLLMTVE
                     DLDDDEAAVEHMASSDEVTALYAGLFSVELLHAEYHEAVQGRGDGVVDGRCIHKVYRM
                     RREARSPSGTGGTDA"
     gene            7970..8875
                     /locus_tag="PLH85_13715"
     CDS             7970..8875
                     /locus_tag="PLH85_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNB65669.1"
                     /translation="MIRLRLSVELSYDVAPPGCDVIFNVHAAHTSHQRVATEMMEISQ
                     FLLPQVHTDVATGNRYMRVKAEAGGLRLRYSATVEVRHHRAVPDGIHEVPVSQLPTGA
                     LIYLYPSRYCQSDRLYDMARQTFGHLLPGYARVQAICDWVRAHVKFQSATSTASTSAL
                     DTLRDKVGVCRDFAHLMIALCRALNIPARFATGIDYGADATLGPHDFHAYVEVFLGDR
                     WYLFDPSGTAIPMGLMRFGTGRDAADVSFATLFGTVNAAPPRIDITAIEDAATGALLP
                     WRCPDALSTDDGIGMLVGTRLVSAV"
     gene            8924..9445
                     /locus_tag="PLH85_13720"
     CDS             8924..9445
                     /locus_tag="PLH85_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65670.1"
                     /translation="MSTIDAIAARHGFSADAGRAAYSALQAGGFTQAQFSHPELGGMG
                     QWMSGGMMMIGDMFNDGLKWRVGALLTELAQHREPAPAMAPMGGGFAQWSNWWPAEFG
                     SPSTSGSQNDMAYAWFPHMAKLLVKLGGDVFAFDTGNLSIYGVQQQQGGGIASVVLNT
                     SNGPMGLLGLNRG"
     gene            complement(9554..9829)
                     /gene="infA"
                     /locus_tag="PLH85_13725"
     CDS             complement(9554..9829)
                     /gene="infA"
                     /locus_tag="PLH85_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNB65671.1"
                     /translation="MAKEELIEMQGVVSEVLPDSRFRVTLDNGHDLIAYAAGKMRKHH
                     IRIIAGDKVSVEVSPYDFSKARITFRHIEGRHAAPPPPAGFTGKRRH"
     gene            complement(9928..10134)
                     /locus_tag="PLH85_13730"
     CDS             complement(9928..10134)
                     /locus_tag="PLH85_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB65672.1"
                     /translation="MATGIVKWFNDSKGFGFITPDAGGDDLFAHFSAIQSKGFKSLKE
                     GERVEFDVTTGPKGQQASNIRPAN"
     gene            complement(10404..14444)
                     /locus_tag="PLH85_13735"
     CDS             complement(10404..14444)
                     /locus_tag="PLH85_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB65673.1"
                     /translation="MTRGSLRLYAAPLIAVLAIIALCTHVVLAQRRESQTSAETAARN
                     AAVVLADQLQSAFDQTDALLVSVGQRYVRARLDGPIGLARDIEPIRGEMPHHPLIKRI
                     GIADEQGIVVFNTGYTPATTDRPNVSARSYFKAAQAGEKGLMFDGPLQAKLDGVWVIA
                     LARRIDGPRGEFLGVVFAILPVESIGRLISRVELGSAGIVNLRTLDLAQVVRRPELQG
                     PGRDVGNQNVSQTIRDLLRDAPGRDRYVYTTVAPIDNTERVYVYQKLPQAPFWMTVGL
                     ATADFATSWQQTGALLALLGAVVAWTLLRATRRIVHQNRDLDRRLLEKEAAEAAARSS
                     EQRLRTILDGVDAAIYLKDPAGRYLFANAAMCRMLGLALDDVIGADDGRLFDDATAAR
                     LRADDRRVLDDGETVHGEETRTLRAGGQSATYLTTQLPLRRPDGSIEALCGISTDISE
                     RVRDRIALQSSESRFRAVVEQALVGIFISQGGIFKYANPRCVEMLGYTDVSELVDRVS
                     VLDHIDPAQRAQVADNIRRGEGGEGGALHDLDLVMLTRDGRPVDVEAYAVNTEVDGRP
                     ARLAVILDVSERARLMRELDQHRHHLEQLVQERSAELHVQAREYEDLYNGAPCGYHSL
                     GPDGVFQRINDTELGWLGYTRDELVGCRAIPEIMTPASQAVFRENFPRIAAGATIKDL
                     EIELIRKDGSVFPVLVSASPQFGPDGQMICTRSILVDYSNLRQQRQTLRRVLTASPMA
                     VRIARASDSRVLFMNKAFCDLVHQDEVAALELDVSAFYVDPGVFGDIRNALGRGETIL
                     NRLVELHLPLQPEAPHVWVLASYMPIEYDGQPAALAWLFDVTELQEARTAAEAATRAK
                     STFLANMSHEIRTPLNGVLGLAQIGFRDSAGRKAQQTFSGILDSGKLLLTVINDILDF
                     SKIEAGKLDIEDVPIDPARIVETALRSVRPQAEAKGIALEAVPDGLPSACLGDPSRIS
                     QVLLNLLTNAVKFTERGEVVLRACVREGALVFKVADTGIGIAPDVLGRLFRPFEQADG
                     SITRRFGGTGLGLVISRRLAELMGGTLDARSKPGAGSTFILRLPLRLTDRPVAPGHTP
                     ATGGRQRLKGLRLLVAEDNTVNQLVIDNILQAEGALVQIVDDGLAAVEAVARSAQPFD
                     AVLMDVQMPRMDGLEATRQICQASPGLPIIGQTAHALRSEHERCLDAGMVATVTKPID
                     IEVLVSALLEHVGGAGRIALPAPEPRPTPVPEAIIEWTALSDRYPGMGTLIDRITTLF
                     VAHHAHDGERLRQMADAVDLHALEALAHELKGVGGTLCAPEVERLAVRTLAAARAGTA
                     DAVELARALADALERLLDAVQQRRAG"
BASE COUNT         2332 a         4624 c         5451 g         2480 t
ORIGIN      
        1 ccccagcggg cggcccagcc tgccggcgca gggtgattgc attgattgca tcgcccgcta
       61 caatgccccc atccccacct ctccgggtgc cccccatggc caccaacctc gtcgtccgca
      121 acatcgaccc cgccctcgtc caggccctca aggaagccgc cgccgcccac ggccgcagcg
      181 ccgaggccga acaccgggaa atcctgcggg ccagcctcgc ccgcccgccg cgccgcagcc
      241 tcaaggacgt cctcgccgac atgcccgacg tgggcctcga cgacgacttc aacctgcgcc
      301 acggttgatg tacctcctcg acaccaactt cgtcagcgag ctgcgcaaag gcccccgcgc
      361 caaccccggc gccctcgcct tcttcgccac cctgcgggcc gaagacctct acctccccgt
      421 gcagaccctt ggcgagatcc gccggggcgt ggaaaacctg cgcggccggg gcgaccaccc
      481 ccaggccgaa cgcctcgaag cctggctcga ccggctcatc gccgagcacg ccccgcgcat
      541 cctcgacttc gaccgggact gtgcccagat ctgggggcgg ctcctctccc cccatcccca
      601 gcaccccatc gacaagcaga tcgccgccat cgccctgctc tacgacctca ccgtcgtcac
      661 ccgcaacagc gccgacttcg cccccctcgg cgtgcgccac cacaacccct tcagctgaca
      721 cgcccgccgc cctgcgcaag gctccgcccc ttgcgcgcgc agcgccgaag cttgcgcgca
      781 aggcaaaaag ctttgcccgc aagcccccca agctcgcccg caaggccacg acgcttgcgc
      841 gcaagccggc gaggctcgcg cgcacagcac aaagccttgt ccgcaaggca aaaagccttg
      901 cgcgcaacgc tcacaggctt gcgcgcaagg ctcgctggca ggtccgcaaa gctcggcgcc
      961 ttgcacacaa ggaaaaaaga ctcgcgcgca cagccccgag ccttgcgcgg ggcgcggcgt
     1021 ccggcggggt ctgcaaggcc gtgtccgggg gctcgcggcg ccccggaacg ggggcttcag
     1081 gcggcaaagg cccgggcgag gagcgcgtcg aagctggcct gggcctgggc ggtggcggcg
     1141 gcttgttggg attggagggc gcggagagtt tcgaaacgga aaacaaaagc ctcttgcaaa
     1201 ttttttggtg ggacggaaat tgggaggttt cttaaaattt ccagatttat gtttttttgt
     1261 gctgcctgtg gagcatgcat ttcaagagtt ggctgcagaa aatccagcca tgtctgtaca
     1321 taacgcgtac acaccatatc acctgccacg aatccgacaa cgctatccgg aaagcaagca
     1381 gagatattca atacagcagt tctggcaata ttcgctgcaa tcgttatgca cagcgtacct
     1441 ggcgcccaca acttactctg cgaaagccct tgctctgaat acgtatttac acaagaagaa
     1501 atcgttccac cggaattaga aacatcgcct gtctgaatga acggataagg cccaccatat
     1561 aattccgcag catcgcgggg tctatgccga gaacgcccac gatcaagcac cccgacctcc
     1621 gaaaacaggt gttgcctcca ccccttagga tttgtcacag gatcaccaaa catctggaga
     1681 aacagcgcag gaaccagttc ggcagtcttc ttctcggcct cgcggcgcag gcggaggatg
     1741 ctttcggcgc gggtgaggag atcgacaata cggctttgct cggacaaatc gcgcaacaag
     1801 aatggcaacg acagaacgaa tgccatatca gcacgcggca tgcgcgaacc agcggttttg
     1861 tcagaaatta ctcgaacaac gtcagggcag cggaggaaat aggccagata tcgacgatca
     1921 actgttgcat tgactttcaa cggaacaatc tccgttgagc actttccctc aaaatccggc
     1981 aaagcaacct tgttcaaata cggacgcaat ttcccataaa gcacatgttc cggaccgaat
     2041 cgaaaactgt tggcaagtgg tgattcagga gtctttgaca agccaccttc gagaaagcgc
     2101 ccagtccctg actcgatgga ctccattccc acatagcgaa gcgccgcacg ctcaccaggt
     2161 ttgatcgatt ctcggacttg cgagcacacg gtccccagtc tgacgactgt cattcccccc
     2221 cctcccccaa cagcgcccgc agtgcctcca cctcttcgac gatctcggct tcgatggcgg
     2281 ccagttcggc caggagcagg cgcgggtcgc ggtgttccac ggtgctgcgg gtctggggcc
     2341 ggtagcggcc ggcggagagg ttgtagtcgt tggcgcgcag ggtgtccacg tcggcaaacc
     2401 accagcgttc ggtccagtct gcgtcggcgg ggcggcgctg ccagcgggta cgggcggtgg
     2461 cgcgctcggc ataggcgctg agcagggccg gcaggtcgtc ggcgtcgatg ggggtgtcgt
     2521 ggttggcgtc gagcttgtag ccgtcctgct cggcgtggag gaagaggacg tcctgggtgc
     2581 ggccgccgcg gcggaagacg aggacggagg tcttgacccc ggaatagggc tggaagacgc
     2641 cgccgggcag gctgaggacg gcgtcgacgc ggttgtgttc gatgagctgg cggcgcagtt
     2701 cccggtgggc gccggtggag ccgaagagga cgccttcggg gacgatgacg ccgcagcgcc
     2761 cgccgccggc gccgctggcg gggtcgctgg gggcgcgcag gctgtcgagc atgtatttga
     2821 ggaagaggag ttcggtggcg gtggtgctgc cgaccttgac gtcttcgacg atgcggtcct
     2881 tgtccacgcg gccggagaac ggggggttgg cgaggatgac gtggaagccg tcggcgggca
     2941 ggccgcgctc ggctttttcg gcggcgtcga gggtggtggt gaggacgttg cgcttgagga
     3001 tgtggacgtc cggcaggccg cgcagggtga ggttcatggt ggcgaggcgg accatcttgg
     3061 ggtccacgtc gttgccgtag aaggtggcgc tggcgagggc atccacctgg gcggcggaga
     3121 ggcggtcgcc gaggccgcgg cgcacgggtt tgccgtccac ttcgtcctgg tggatggcgg
     3181 cggcgctgga gtgggccagg cgcatgtggt cgtaggcggc caccaggaag ccggcggtgc
     3241 cggcggcggg gtcgtagacg gtttcgccga cctgggggtc gagcaggcgc acgatggcgc
     3301 ggatgatgtg gcgcggggtg cggaactggc cgagttcgcc ggcctgtttg atctggcgca
     3361 ggacgtgttc gaagaggtcg cccttggtgt cggcgtcgga ctggtcgagg cgcaggccgt
     3421 ccacgagggc gatgacctgg gtgaggacgg tgggttcgtc gatgctgaga cgggcgccgt
     3481 ccatgaagtt gtgggcgctg cgggcgccga gctcggcgta gaaggggaag acttcgtcgc
     3541 gcaggaagcg gacgagggct tcgccggagt agcccttggc ccatacggac cagcggaagt
     3601 gggcgcgggg caggctcttc tgcccttcga cgggggcgtt gagggggttg cggaggtccc
     3661 actcgccggc gaacagggac tggtaggggc gtttgaggac gcgggccttg tggccgttgg
     3721 cggtgtcgat gccttcgacg aggtagaaga agaagaggaa ggcgagctgt tcggcgttgc
     3781 tgacggggtc ggggtagccg ccgccgaaga ggtagtcgcg aatctggtcg atggaccgcc
     3841 gcatgtcggg ggtcaggggc atgggtgttc cttgggggtg atgcggggat tacggggcgg
     3901 tcgggcgggg tgcgtcgtcg gggccggggg cggggtcggc ggaggcgtcg tctagggggc
     3961 tgaagacggc ggcgttgagg gcaccgagca gggccgccag gcgggcgtcg ccacccagga
     4021 cacgacgggc ttcggcgagg ccgcccatct gggcgaagac gtggaagtag gcgaagtgct
     4081 cgggggtgat ctcatcgagg gtgtcggcgt tggcgcggat ctggttgccg aggaggtgga
     4141 gccagcgctc ggcttcggcg gaaaggtcgg cattgtgcag catccaggct tcgaagcggg
     4201 tgccgagttg ggcggcacgg cgttcgctgg cggcgggaaa gacgaggttg ccgtcggcgt
     4261 cgagggtgat ccattcgcgg ctggcggggt cgatggtgtc gtcgatgggg aggccgagca
     4321 ggcggcgcgg actgtagcgg cgccgggcgg gggtctcgcg gacgaggccg ccttcgccgt
     4381 aggtgtcggc atcgccgtgg aagtcgacga cgccgacgaa gtcgaagagg gtgaagacgg
     4441 gtttgttggg ggcccggcgg gtgccccggc cgcgcatctg ctggtagagg atggtggagc
     4501 gggtgaagcg ggcgaagacg aggttggcga cgtcggcgca gtcgaagccg gtgtcgagca
     4561 tgttgacgga gacgaggatg cgggggaatt cctggcgctt gaagcgctgg atcttggtga
     4621 ggccgtcggt cgtgtcgtcg tcgccgcagc cggacacgac gtagtcggcg taggccacgg
     4681 cggggtcggg cttgcagtcg gcgaaggcgt cgtcgaagag cttggcgagg gtggcggcgt
     4741 ggcgcttggt gacggcgaag acgatggtct tgccaagcag aggcttgcgc aggatgcctt
     4801 tgtggtcacg gaagccttcg tccatgacct tgcggaattc ggtgacgatg gcctggttgc
     4861 gggcggggat ggtgaagcgc cgttcgaggg cgttggggtc gacggtgagg gtgtcgtcgg
     4921 gaccgaaaag ctgggtaagc tcggtgcgcg tgtcggcgtc cagggcgttc cagtccagtt
     4981 cgccgcgggt gacggtgaag ccgccttcgg tggcggtgcg cacggtgcgg gcacgataga
     5041 tctggtaggg caccaggtgg ccgtcggcga tggcctggcg caggccgtag gagaaggtgg
     5101 gctgctcgac ttcgaagaag cgcagggtgt cgcgcacgaa gaggcggtct tcgtcgtccg
     5161 ggtcgggcgc gcgctcgtcg cccggcgggg cgacgcaggg ggtggcggtg aggccgatct
     5221 ggaggccgtc gaagtggcgc agcaccccgc tccacttgcc gtagatgctg cggtggcatt
     5281 cgtcggtgac gacgaggtcg aagtagccgg aggagtagtc cctgtagagg ttaaccatgc
     5341 tctgcagggt ggtgacggtg atgcgctttt cgtcctggaa gcggcgcccg gcgcgcagga
     5401 cgtaggcggg gaaatccggc aggtgttcgg cgaaggcgtc ttcggcctgg gtggcgaggg
     5461 ggatgcggtc caccaggaag agcacgcggg tgatggcgtg ggcttcgaag aggcgtttga
     5521 tgagggcggc ggcagtgcgg gtcttgccgg tgccggtggc catctccacc agcagcttgc
     5581 ggcggccgcg ggcgatttcg gcgctgaggg tgtcgatgca ggcgagctgg tagtcgcggc
     5641 cggcgatgcg ggtgtcgatg gggacgcggt cgagggggat gcggatctgg cggacggcgg
     5701 cccggcgttc caggtcgtcc tgcccgaaga aggtcttgac cggccggggg tgggcttcgc
     5761 gctgccagtc ccagaacagg atttcgtggc cgttggccag gaagacgtag gcgacgccca
     5821 gttggcgggc gtagcgaccg gcctggtcgc gggcggtggc cgggtcgacc gtgaagcgtt
     5881 tggcctcggt gaccgccagg gcgcgcccgt ggcggtcgtt cagcacatag tcggcgatgc
     5941 cgtcgtcgag ggcgacttcg taacagacgc tgttggggtt gtgcagatcc cagccctggt
     6001 cggcaagctg ggcgttgatg gcgacacggg cgtgggcttc gttgtcgtgc atggccggcg
     6061 attccattgg agtgccggca tcttaccctg ccgcgggtcg gcgacggcgg gcgagcgccc
     6121 accggccccg tcgaccgcct cagtcgaaca cgtaagacac cgaactgagg ggctgcgccg
     6181 tgtgctggag gcattccagc acctcgaaac gctcggcggc gtcggcttcg gtgaggggat
     6241 agggggtgat gaaggccggg cgggacgcct cgggctggtc gggggacgct ttggctcgga
     6301 cgcagaagcg atagagcacg gggtcttcca tggcagatcc ttctgtgggc ggttggggtg
     6361 cgcggagtat acgccgcagc cggggtgtgc gagtgccggg gcgcaccgcg gcacggacgg
     6421 gatgccgcgc ggtgccgatg ggactacact gccaccgcca tgcttcatta cgccgtcaag
     6481 gtggccgtgt ctgccggcct catcgtcgca atctccgaga tcgccaggcg ctcgtcgggg
     6541 gtcgctgccc tgctggcggc gctgcccctc acgtcggtgc tggcctttgt gtggctgtat
     6601 gtggaaggcc agccgacgga ggcaatcgcg acgctgtcgg ggcagatctt ctggctggtg
     6661 ctgccgtccc tggtgctgtt cgtggccttg ccctggctgc tgcgccaggg ggtcggcttc
     6721 tgggcggcgc tggcgctggc gatggggctg accgccgggg cctatgcggt gttgcggccg
     6781 ctgttgcggc acctgggggt ggagctgtaa gggcggcggc aggcgtttat gccggcgcgg
     6841 ggccggcggt gcggccggcc ccgtgtgggg gatcgcccgt gcgggacggg cgcccggccg
     6901 gtgggtgagg cgctgcgcgc cgccacggca tttgcctcgc gccccggtcg ggcgcatagt
     6961 ggagccgctt gactccgcgc ccggcgccct gaaaagcatt cgccccccgc atcgatggct
     7021 gtttcctgcg ggccctcctc cgcttttcct aggccctccc ccatggctca ccccgaccac
     7081 ctgctctggc gacagggctg gaacgcgaac cggattccct tccatctcac ccacatccac
     7141 ccgctgctgc ggcgcttctg ggacacgctg gacccggccg cgacggcgcg ggtcttcgtg
     7201 cccctgtgcg gcaagagcct ggatctgatg tggctgcacg gcctgggcca cgatgtggtg
     7261 ggcatcgagc tgagcgcggt ggcggtgggg gatttcttca aggcggcgcg cctgcgcccg
     7321 cagcgcaccc gtcaggcgga cctgacctgc tggacgcagg agcggctggc gatcttctgc
     7381 ggggacttct tcgatctgac ggaggaggat ctgcaggggg tgtcggcggt gtatgaccgg
     7441 gcggcgctga ccgccctgcc ggaggggctg cgcgcggatt acgtggccca cctgcatgcg
     7501 atcctgccgg cgggctgcca ggtgctgctg atgacggtgg aggatctgga cgacgatgag
     7561 gcggcggtgg agcacatggc gtcgtcggac gaggtgacgg cgctgtacgc ggggctgttc
     7621 agcgtggagc tgctccacgc ggaataccac gaggcggtgc aaggccgcgg cgacggcgtg
     7681 gtggacgggc gctgcatcca caaggtgtac cggatgcgcc gcgaggcccg cagcccctcg
     7741 ggcacgggcg gtaccgacgc gtaggcggcc ccgcacgggg tgtgcgcacc cgccggacag
     7801 ccccgccggg cggtgattcc ggaaaaaacg cagcatgatg cggtgccgca gggtaggatg
     7861 agcgccttgt cgcacgttct ggatcgcttg atccggcacg tcgcgacggt cattcagcct
     7921 cgcccggtgg cccgcaatcc tgcgtcaagg cgtgtttccg gtctttttca tgatccgctt
     7981 gcggctttct gtcgaattga gttacgacgt ggcgcctccc ggctgcgacg tcatcttcaa
     8041 cgtccacgcg gcccatacgt cccatcagcg ggtggccacc gagatgatgg agatcagcca
     8101 gttcctcctg ccccaggtcc acaccgatgt cgccaccggc aaccgctaca tgcgcgtgaa
     8161 ggccgaggcg ggcggcctgc ggctgcgcta tagcgccacc gtggaagtgc gccaccaccg
     8221 ggcagtgccc gacggcatcc acgaagtgcc ggtgtcgcaa ctgccgacag gtgcattgat
     8281 ctacctgtac ccgagccgct actgccagtc cgaccgcctg tacgacatgg cgcgccagac
     8341 ctttggccat ctgctgccgg gatacgcccg ggttcaggca atctgcgatt gggtgcgggc
     8401 tcacgtgaag ttccagtcgg ccacctccac ggcgagcacc tcggcgctgg ataccctgcg
     8461 ggacaaggtg ggggtgtgcc gggatttcgc tcatctgatg atcgccctgt gccgcgccct
     8521 caacattccg gcgcgctttg ccacgggcat cgactacggc gccgacgcga cgctggggcc
     8581 ccacgacttc cacgcctacg tggaggtctt cctgggcgac cgctggtatc tgttcgaccc
     8641 ttcgggcacg gcgatcccga tgggactgat gcgcttcggc accggacgcg acgcggccga
     8701 cgtatccttc gcaacccttt tcggcacggt caacgctgca ccgccccgaa tcgacatcac
     8761 cgcgatcgaa gacgccgcca cgggtgccct gctgccctgg cgctgcccgg acgccctgtc
     8821 caccgacgac ggcatcggga tgctggtagg cacgcgcctc gtctccgccg tctgagggcg
     8881 gagcgcatcc catttacacc catttcaatc aggagtactt cccatgagca ccatcgacgc
     8941 cattgcggcc cgccacggtt tcagcgccga cgccggtcgc gctgcctatt cagccctgca
     9001 ggccggcggc ttcacccagg cccagttcag ccacccggag ctgggcggca tgggtcagtg
     9061 gatgagcggc ggcatgatga tgatcggcga catgttcaac gacggcctga agtggcgcgt
     9121 cggggccctg ctgaccgagc tggcccagca ccgggagccg gcaccggcga tggcgccgat
     9181 gggcggcggt tttgcccagt ggtccaactg gtggccggcc gagttcggct cgccgtccac
     9241 ctcgggcagc cagaatgaca tggcctatgc gtggtttccc cacatggcca agctgctggt
     9301 caagctgggc ggcgatgtct tcgccttcga taccggcaat ctgtcgatct atggcgtgca
     9361 gcagcaacag ggcggcggga tcgcgtcggt ggtgctgaac acctccaacg gccccatggg
     9421 tctgctgggc ttgaatcggg ggtgaagacc ggggcgctgc ctgtgcggcg gcgcagcccg
     9481 agccccggcg ggcgccgggc gtccgacacg gaccctgacg cggagcgccg ggtacatgtc
     9541 gcgggcgtcg cgctcagtgg cgacgcttgc cggtgaagcc agcgggcggc ggcggtgcgg
     9601 cgtggcggcc ttcgatgtgg cggaaggtga tgcgcgcctt gctgaagtcg tagggggaga
     9661 cttcgacgga gaccttgtcg ccggcgatga tgcggatgtg gtgcttgcgc atcttgcccg
     9721 ccgcgtaggc gatgaggtcg tgaccgttgt ccagcgtcac gcggaatcgg gagtcgggca
     9781 ggacttccga gacgacgccc tgcatttcga tgagttcttc cttggccatg ggggtccttg
     9841 cagtccgtta acgaaaaagc ccggctgggc cgggcctttc aatccgcctc gggatgggga
     9901 cagatgccac accccgatgg acgacactca gttcgccgga cggatgttgg aggcctgctg
     9961 gcccttggga ccggtggtca catcgaactc cacgcgctca ccttccttca ggctcttgaa
    10021 gcccttggac tggatggcgg agaagtgggc gaagagatca tcgccgcccg cgtccggggt
    10081 gatgaaaccg aagcccttgg agtcgttgaa ccacttaaca atgccggttg ccatgtcttg
    10141 aatatcctga aataaataac gcaaagggga tttgcccctg aaaaatggga cgacactcaa
    10201 gacagcgggt acttattggg aggaaccgca ggactgcgga atacgacctg aaagctacgg
    10261 cactacttgg gaatcgcccg cgcactatgg gggacaacgg ggcaaatagc aacaagaatc
    10321 ttgcatcaag ggctgtttgt ttgcgcgcgg gccccggatc gcccaatgac accgccccga
    10381 ttgccccgac gagggccgcc accctacccg gcccgccgtt gctgaacggc gtccagcagg
    10441 cgctcgaggg catcggccag ggcgcgtgcc aactccacgg cgtcggccgt acccgcgcgg
    10501 gcagcggcca gggtgcgcac ggccagacgc tccacttcgg gcgcgcagag ggtgccgccg
    10561 acgcccttca gctcgtgggc cagcgcttcc agggcatgca gatcgaccgc gtcggccatt
    10621 tgacgcaggc gctcgccgtc gtgggcgtgg tgcgcgacga acagggtggt gatgcggtcg
    10681 atcagggtgc ccatgcccgg gtagcggtct gacagggcag tccactcaat gatggcttca
    10741 ggcactggcg tgggacgcgg ctcaggcgcg ggcagcgcga tgcgccccgc accaccgacg
    10801 tgctccagga gcgccgagac cagcacttcg atgtcgatgg gtttggtgac ggtggccacc
    10861 atgccggcgt cgaggcagcg ctcgtgctcg ctgcgcaggg catgggccgt ctggccgatg
    10921 atcggcaggc cggggctggc ctggcagatc tggcgggtcg cttcgaggcc gtccatgcgg
    10981 ggcatctgca cgtccatcag caccgcgtcg aagggctggg cgctgcgggc gacggcttcc
    11041 acggcggcga ggccgtcgtc cacgatctgg accagagccc cctcggcctg caggatgttg
    11101 tcgatgacga gctgattgac ggtgttgtct tcggcgacga gcagacgcag gcccttgagg
    11161 cgctgccggc ctccggtggc cggcgtgtgg ccgggcgcta ccggccggtc ggtcaggcgg
    11221 aggggcaggc gcaggatgaa ggtgctgccg gcgccgggct tgctgcgggc gtcgagggtg
    11281 ccgcccatca gctcggccag gcgccggctg atgacgaggc ccaggccggt gccgccaaag
    11341 cggcgggtga tgctgccatc ggcctgttcg aagggacgga agagccgtcc gaggacatcc
    11401 ggcgcaatgc cgatgccggt atcggccacc ttgaagacaa gcgctccttc ccggacacag
    11461 gcccgcagga cgacctcgcc ccgctcggtg aatttgacgg cattggtgag cagattgagc
    11521 aggacctggc tgatccggct aggatccccc agacaggcgg aggggagccc gtcgggcacg
    11581 gcctcgaggg cgatgccctt ggcctcggcc tggggccgga cgctgcgcag ggcggtctcg
    11641 acgatgcgcg ccgggtcgat gggcacatct tcgatgtcga gcttgccggc ttcgatcttg
    11701 gagaagtcga gaatgtcgtt gatgacggtg agcagcagct tgcccgaatc gaggatgccg
    11761 ctgaaggtct gctgggcctt gcgcccggcg ctgtcccgga agccgatctg ggcgaggccc
    11821 agcaccccgt tgagcggcgt gcggatctcg tggctcatgt tggcgaggaa ggtgctcttg
    11881 gcgcgggtgg cggcttcggc ggcagtgcgg gcctcctgca attccgtgac gtcgaagagc
    11941 caggccagcg cggcgggctg gccgtcgtac tcgatgggca tgtaggaagc gagcacccag
    12001 acatggggcg cttccggctg caggggcagg tgcagctcca ccaggcggtt gaggatggtc
    12061 tcgccccgcc ccagggcgtt gcggatgtca ccgaagacgc ccggatccac gtagaaggcg
    12121 ctgacgtcca gttcgagggc cgccacctcg tcctggtgca ccagatcgca gaaggccttg
    12181 ttcatgaaga gcacgcggct gtcggacgcg cgggcgatgc gcacggccat cggcgaggcg
    12241 gtgagcaccc ggcgcagggt ctggcgctgc tgacgcaggt tggagtaatc caccagaatg
    12301 gagcgggtac agatcatctg cccgtcgggc ccgaactggg gcgacgcgct caccagcacc
    12361 gggaagacgc tgccgtcctt gcggatgagt tcgatctcca ggtccttgat ggtggcaccg
    12421 gccgcgatgc gcggaaagtt ctcgcggaac acggcctgcg aggcgggggt catgatctcc
    12481 gggatggcgc ggcagcccac cagttcgtcc cgcgtgtacc cgagccagcc cagctcggtg
    12541 tcgttgatcc gctggaagac cccgtcggga cccagggagt gatagccgca gggcgcgccg
    12601 ttgtagagat cttcgtactc ccgggcctgg acatgcagtt cggcgctgcg ctcctggaca
    12661 agctgttcca ggtggtgccg gtgctgatcg agctcgcgca tcaggcgcgc ccgttcgctg
    12721 acatcgagga tcacggccag gcgcgcgggc cggccatcca cctcggtatt gacggcatag
    12781 gcctccacgt ccaccgggcg gccgtcacgc gtcagcatca ccaggtccag gtcgtgcagg
    12841 gcgcccccct cgccgccttc gccgcgccgg atgttgtccg ccacctgggc acgctgggcc
    12901 ggatcaatat ggtccagcac gctgacccgg tccacgagtt cgctcacgtc ggtgtagcca
    12961 agcatttcga cgcagcgggg gttggcgtat ttgaagatgc ccccctgact gatgaagatg
    13021 cccacgaggg cctgttccac gacggcccgg aagcggctct cggaggattg gagcgcaatc
    13081 cggtcccgga cccgctctga gatgtcggtg gaaatgccgc acagggcttc gatgctgccg
    13141 tcggggcggc gcaggggcag ctgggtggtg aggtaggtgg cggactggcc gccggcccgc
    13201 agggtccggg tctcttcgcc gtggacggtc tcgccgtcgt cgagcacgcg ccggtcgtcg
    13261 gcccgcaacc gggctgcggt ggcgtcgtcg aagaggcgtc cgtcatcggc accgatcacg
    13321 tcgtcgaggg ccaggcccag catccggcac atggcggcgt tggcaaagag gtagcgcccg
    13381 gcggggtcct tcaggtaaat ggcggcgtcc acgccatcga ggatggtgcg caggcgctgc
    13441 tcgctgctgc gggccgcggc ctcggcggcc tccttttcca gcagacgccg gtccaggtcg
    13501 cggttctggt gcacgatgcg gcgcgtggcg cgcagcaggg tccacgccac gaccgcgccc
    13561 aggagggcca gcagcgcgcc ggtctgctgc cagctggtcg caaagtcggc cgtcgccagg
    13621 ccaaccgtca tccagaacgg cgcctgcggc agcttctgat agacatagac ccgctcggtg
    13681 ttgtcgatgg gggcgaccgt cgtatagacg taccggtcgc gaccgggcgc gtcccgcagc
    13741 aggtcgcgga tggtctggga aacgttctgg ttgcccacgt cccggcccgg cccctggagt
    13801 tcggggcgtc gtaccacctg ggccagatcg agggtgcgca ggttgacgat gccggcactg
    13861 cccagctcca cgcgggagat gagccggccg atggactcca ccggaaggat cgcaaagacc
    13921 acgccgagga actcgccccg gggaccgtcg atgcgacggg ccagggcaat gacccatacg
    13981 ccatcgagct tggcctggag cggcccgtcg aacatcaagc ccttttcgcc agcctgcgcc
    14041 gccttgaaat agctgcgggc ggaaacgttc ggacggtcgg tcgtggccgg ggtatagccg
    14101 gtgttgaaca ccacgatccc ctgctcgtcg gcaatgccga tgcgcttgat gaggggatgg
    14161 tggggcattt cgccgcggat gggctcgatg tcccgggcga gcccgatcgg cccgtcaagc
    14221 cgcgcacgga catagcgctg accgacggag acgagcagcg catcggtctg gtcgaaggca
    14281 ctctgcagct gatcggccag caccactgcc gcattgcgcg cggcggtctc ggcgctggtc
    14341 tggctttcgc ggcgctgggc cagcaccacg tgggtgcaga gggcaatgat tgcgaggacg
    14401 gcgatcaggg gcgccgcata gagccgcagg ctcccccggg tcatcgatgg cgtgcggtgt
    14461 gtggtccggt ctggtatctg catgctctgg ctcagtccgg ctccgccgcc ggcgcttcgt
    14521 gtcggacggg acgcggacgt cccgcggaca gcctgccttc cccgccgcat gctccacggc
    14581 gggcgatccg ctggaacaca tcgctcaccc gcccccgcgg gggtcaggac ttctgtccga
    14641 agggcttatc ggcaccagat gaccactctt cagccggatt tgacgaaaac gtcaaacagc
    14701 gcacgaccgc ctgcagactc cggacatcga tgcgcgcccc gccggctgag gcgcggtgcg
    14761 atcagggctt gcgagcctca ccggggcccg cggaagcggg atcggcggac gctgtgcgcg
    14821 ccgggacgac cccctgcacc atgtcgccct ccgccagcgc gtccggcgga ttgaggacga
    14881 ggcggtc
//
LOCUS       DAOQMM010000096        14440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1710_length_14440_cov_49.423497, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000096 DAOQMM010000000
VERSION     DAOQMM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1710_length_14440_cov_49.423497"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..834)
                     /locus_tag="PLH85_13740"
     CDS             complement(1..834)
                     /locus_tag="PLH85_13740"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNB65674.1"
                     /translation="MATYAIGDIQGCFSALQALLKRVEFDAGHDRLWLVGDLVNRGPD
                     SLKTLRFVRDLGPAAVTVLGNHDLYLLMVAYGTVRSRGKDDTIQAVLDAPDREALLGW
                     LRTRPLMHVENGFAMVHAGLLPGWTVSQARALAREVEGALAGPYHADLLHNMWGSEPA
                     AWRDDLRDYERMRVIVNAMTRMRFCTPEGVMDFKVKGEVARAPKGYLPWFDAPARRST
                     DATVVFGHWSALGLKIRPNLMALDSGCLWGRELTAVRLEDRQVFQVSCPGLSHGKPQP
                     S"
     gene            complement(844..2727)
                     /locus_tag="PLH85_13745"
     CDS             complement(844..2727)
                     /locus_tag="PLH85_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNB65675.1"
                     /translation="MRSLQKFAHPLLVFLSDALGICLAWFVAYLIRFNLAIPAEFMPA
                     MLYGLAVALPIQCLFLRVFGLYRGIWVFASLPDLHRIGRAVAAGAVVVPLVLVVSVRT
                     EPWIPRLVFLLQPVLLLLYMGGTRALYRSWKEFRNFGALRAQGQSVLILGAGVAGAHL
                     VRELAASAQWRVVGLLDDDPHKQRREIHGYKVLGPISELERWAETLKVGHAIIAMPSV
                     RHEVRRAVASECVRVGVTALIVPGVDERLSTSHSLSEVRQLDVEDLLGRDPVRIDTEQ
                     VQDYLRGKAVMVTGAGGSIGAELCRQIARFEPSVLVCFELSEFALYRLIEDFAREFPR
                     LQLVALAGDVKDAVRVDEVIGRYRPNVIFHAAAYKHVPLMEEDNAWQAVRNNVMGTFQ
                     VARAAVAFGVPRFVLVSTDKAVNPTNVMGATKRLAEQVCQAFALEGRSQFEIVRFGNV
                     LASAGSVIPKFQAQIAAGGPVTVTHPEITRYFMSIHEAAQLVLQAGSMGQGGEIFVMD
                     MGEPVRIQDLARDMVRLSGRSEDDIRIVFTGLRPGEKLYEEVLADSERTRPTHHPKLR
                     IARAQAVESGWLDMLALWLRQRRAVPAAEVRRELRRWVPEYTPSSHAVPLTKVSDTGR
                     QRA"
     gene            complement(2738..3715)
                     /locus_tag="PLH85_13750"
     CDS             complement(2738..3715)
                     /locus_tag="PLH85_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB65676.1"
                     /translation="MLAVGLAFGAAWGVTRTCCRPDSLLHFVDQPNERSLHVRPTPRT
                     GGVGVMAGVLLGGGLALLAGHGSMGLVLALAGAVLLAVVGLADDRLDLSARVRLLVHL
                     GVAAGFVVISDAGGGVEAWRVLPLVLGLVWMTNLYNFMDGSDGLAGGMAVWGFVAYAV
                     AAGGHGDGTLAAVCASIAAAAAGFLCFNFPPARIFMGDSGSVPLGFLAGALGCVGVMG
                     NRWPAWFPVLVFSPFIVDASVTLARRALRGERVWQAHRTHYYQRLVQMGYGHRGVAVR
                     AYGLMAACGGSALLVMDRGVALQCFVITMWTFVYMVVGFRIDRAWRRSG"
     gene            complement(3726..4688)
                     /locus_tag="PLH85_13755"
     CDS             complement(3726..4688)
                     /locus_tag="PLH85_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB65677.1"
                     /translation="MSGRILVTGGRGFIGRRLVTALQADGACPRVLTRQTPAAPSEVQ
                     GDVTDVTALARACEGVETVFHCAGHAHAFKTHAADEIARHRAVNFEGSVRLIDAAAAA
                     GVRRLVFLSSVKAAGEPGDAQADETWSAAPEGAYGAAKRAAEAALLEAGGRHGLHVVI
                     LRPVMVYGAGGRGNLERMARLVRRGCFPPLPETGNRRSVIHVDDVVSALRVVASDPRA
                     AGRTYILAHPIACSGRALYDELRRVMGLAPIAWAVPARLLQAAAWLADGCGGLAGLHL
                     PFNGEVLDRLLGSACYSSERIRTELGWQARVALADGLKEMLGGR"
     gene            complement(4685..5548)
                     /locus_tag="PLH85_13760"
     CDS             complement(4685..5548)
                     /locus_tag="PLH85_13760"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HNB65678.1"
                     /translation="MNDLDQRRRRGLKAGLALLAGVMLGRAQAATPPPLLLAEVLHGD
                     VDVTRYWVSEKLDGARAIWDGQTLRFRSGREVPAPAWFIAGLPPEPLDGELWMGRGTF
                     DALAAAVRRQPARDDEWRRVRLMVFELPGGAGSFTDRAARIREIVARADVPWLQAVAQ
                     FRVPDRPALQARLDAVVRAGGEGLMLHRDDAPYLTGRHDALLKLKPQLDAEATVIAHL
                     PGKGRFSGRLGALEVETPDGVRFRLGSGFSDAVRRDPPALGSRVTYRYRSLTPGGVPR
                     FASFLRLRVEF"
     gene            complement(5545..6333)
                     /locus_tag="PLH85_13765"
     CDS             complement(5545..6333)
                     /locus_tag="PLH85_13765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB65679.1"
                     /translation="MDRLTVSIVLYRPDARLLQATLDALGEACREAALTPELFLIDNG
                     GGAAIMASLRWPADWTAHVLTGHGNVGFGCGHNLSLAGAGDIHLILNPDLEMAPDALR
                     AARDFLRGHPECGLVVPAARWDDGRIQYLCKRRPAVFDLFLRGFAPAWLRRRFAARLA
                     RYEMQDVIGDAVVWDPPLVSGACMFFRADVLRRVGGFDPRFFLYFEDFDLSLRVARIT
                     RIAYVPAVRVVHHGGHAARKGWHHVRLFMQSAARFYGLHGWTWR"
     gene            complement(6392..7399)
                     /locus_tag="PLH85_13770"
     CDS             complement(6392..7399)
                     /locus_tag="PLH85_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNB65680.1"
                     /translation="MKILVTGAAGFIGMHTSQLLLARGDQVVGLDNLNDYYEVALKHN
                     RLARLTPHENFRFVKMDVADRDGMAKLFAEEKFDRVIHLAAQAGVRYSLINPHAYVDS
                     NLVGFMNILEGCRHSQVEHLVYASSSSVYGGNTRMPFSEHDSVDHPVSMYAATKKANE
                     LMAHTYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVFNHGKMKRDF
                     TYVDDIVQGVVRTLDRVAEPNPAYDADTPDPGTSNSPFRVFNIGNNNPVELMSFVEAI
                     EKALGKTAEKNFLPLQDGDVPATYANTDALNAWTGFVPATPVDVGIGRFVEWYRSYYK
                     V"
     gene            7576..8856
                     /gene="waaA"
                     /locus_tag="PLH85_13775"
     CDS             7576..8856
                     /gene="waaA"
                     /locus_tag="PLH85_13775"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNB65681.1"
                     /translation="MILRFLYTLLWLLAAPFIVLRLVWRARRQPEYLQHLGERLGRYP
                     QRLSLPVIWIHAVSVGETRASAPLVQALLDAYPDHSLLLTHMTPTGRATGEELFGRFA
                     PRVQSAYLPYDLPWVMRRFLRHFRPSMGILMETEVWPNLVETCRRRHIPLALVNARMS
                     ERSARGYRRAGPLAREAFAHLSVIAAQTESDENRMSALGARYLKVTGNIKFDIMPPTT
                     MQALGHEFRNRFGPRQVLLAASTREGEEELLLETFARLCPPEVLLVLVPRHPQRFGEV
                     VKLVRKAGLGLQRRSSNDPVLADTRVWLGDSMGEMFAYYHASDVALIGGSWLPLGGQN
                     LIEACAVGCPVVVGPHTFNFSAAAEQAIEAGAAVRASDMDDGIGVALQILQDPARRSA
                     MGAAGQAYAQTHRGATERTLDLITPLLRAARPRR"
     gene            complement(8899..9771)
                     /gene="ubiA"
                     /locus_tag="PLH85_13780"
     CDS             complement(8899..9771)
                     /gene="ubiA"
                     /locus_tag="PLH85_13780"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNB65682.1"
                     /translation="MTFAERLSEYEKLMRLDKPIGILLLLWPTLWALWVAGGGRPSPF
                     IVWIFVLGTVLMRSAGCVINDYADRNFDGHVERTRNRPLAAGRVRPAEALVLAAVLAL
                     VSFLLVLPLNTLTLLLSVPAVFLAGSYPFTKRFLAIPQAYLGIAFGFGIPMAFAACLD
                     AVPVEAWVMLLANVFWAVAYDTEYAMVDRPDDLKIGIKTSAITFGRFDVAAVMLCYAV
                     TLGLLAWVGLHTGRGAAFLAGLAVAAGLMGYHFTLIRGRDRANCFRAFLHNNWVGGAI
                     FAGWALDYLLKPVG"
     gene            complement(9768..10337)
                     /locus_tag="PLH85_13785"
     CDS             complement(9768..10337)
                     /locus_tag="PLH85_13785"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNB65683.1"
                     /translation="MLRRSMTLAHFPGRDPFRMTASRAPRPLRGWLSDDSSLTARIRA
                     RCGTFSLHLVSQGKGRPLPDELAALGVHRHGELWQREVLLLADGAPVVFARSLVAAST
                     VPAAWHLLRHLGGRPLAAVLFDDPRVRRSPLEAARLDPRDTRWHRAAAATGRSDLPAL
                     WARRSAFRRAGSPLLITEVFLPAIRSLAP"
     gene            complement(10435..12501)
                     /gene="recG"
                     /locus_tag="PLH85_13790"
     CDS             complement(10435..12501)
                     /gene="recG"
                     /locus_tag="PLH85_13790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNB65684.1"
                     /translation="MSAPPPETGPGPRGWPGVGPQLAGRLARLDIFGPQDLILHLPLR
                     YEDETRVTMLADVRPGCAAQCEVTVLHCEVQLRPRRQLVARAADASGEVSLRFVHFYP
                     SQQKQLAPGARLRVFGEVRPGFFGAEMIHPRVRPVAEGESLPESLTPVYPTTAGLAQS
                     ALRKLIGGAMRHHPPREDLPDEVVRRLDLMALPDALHLLHAPPPSLAAVELEDRHHPA
                     WQRIKLDELLAQQISLRRAYAARRAKNAPPLPAVDTLTRALLTELPFSLTGAQRRAWD
                     EIARDLAAAHPMQRLLQGDVGSGKTCVAALAMLRAAENGRQAVLMAPTEILAEQHYRK
                     LAGWLAPLGLEVAWLSGSRRKREREALIARLAAGELLLAVGTHALIEDPVSLPGLALA
                     VVDEQHRFGVRQRLALREKGRGGAPHMLMMSATPIPRTLAMSYYADLDVSVVDELPPG
                     RTPIVTKLVAEARRDEVIRRVHDACVAGRQAYWVCPLIEESEALQLKTAEESFALLAA
                     ALPDLKVGLVHGRFRNDEKSATMAAFVAGEIHVLVATTVIEVGVDVPNASLMVIEHAE
                     RFGLAQLHQLRGRVGRGAHASACILLYAQPLSQTGRERLKAIYEHADGFVIAREDLRI
                     RGPGEFIGARQSGVPLLRHADLEADADLVAVARDLAETLLRDAPAVADAITARWFGGR
                     AALLKA"
     gene            complement(12509..12889)
                     /locus_tag="PLH85_13795"
     CDS             complement(12509..12889)
                     /locus_tag="PLH85_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB65685.1"
                     /translation="MPKQIIATPHAPAAIGTYSQAVRAGDTVYLSGQIGLDPATMQLM
                     DGFEAQTVRVFENLKAVAEAAGASLADVVKLNLYLTDLAHFAKVNEVMARYFSEPFPA
                     RAAVGVKELPRGALVEADAVLVLG"
     gene            13206..14435
                     /locus_tag="PLH85_13800"
     CDS             13206..14435
                     /locus_tag="PLH85_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65686.1"
                     /translation="MIRTRFRADDFAILVALATSAGGIASAALGGPAGLSLVAGTFTV
                     VALVTAMIVSRRARERWRQQQQAQRESIDSSVREYDMLCDQIAVQSGFQYERLRESLG
                     QMQDVVSSAASKLGGSLAGGQGGNSRDQLRHLVDELLSLAAEEERALQESGLERFAAE
                     TRDALRDFVGTVERLKASGSDIAERFGAMRGKVDAVTRLMTEVNQINSQTELLALNAA
                     IEAARAGEAGRGFAVVADEVRKLAQRTETFSDQIATLLTDIHASIDDVGRAVDVTAAT
                     DVSKARSSEASVSAMGLEMSELNARAMVQSRRISELSAKIHRLVMEGIQSMQFEDIVR
                     QILDKLRQHTDFMGRYAHGFFDAHRDPEQREATSRIQRRVEILRQLLAQSEQSKKDIR
                     FEAVQQTAVHAGEVDLF"
BASE COUNT         2506 a         5188 c         4767 g         1979 t
ORIGIN      
        1 tcaggacggc tgcggcttgc cgtggctgag tccgggacag gacacctgaa agacctgacg
       61 gtcctccagc cgcacggccg tcagctcgcg cccccacaga cagccggaat ccagggccat
      121 cagattcggc cggatcttga gccccagcgc cgaccagtgg ccaaacacca ccgtcgcatc
      181 ggtgctgcgc cgggcgggcg catcgaacca cggcagatat cccttcggcg cccgtgccac
      241 ctcgcccttg accttgaagt ccatcacgcc ttccggcgtg cagaaacgca tgcgcgtcat
      301 cgcgttcacg atcacccgca tgcgctcgta gtcccgcaga tcgtcgcgcc aggcggcggg
      361 ctcgctgccc cacatgttgt ggagcagatc ggcgtgatag ggcccggcca gcgcaccctc
      421 cacctcccgc gccagcgcgc gggcctgact caccgtccag cccggcagca gcccggcgtg
      481 gaccatggca aagccgttct ccacatgcat cagcgggcgt gtccgcagcc agcccagcag
      541 cgcctcccgg tccggcgcgt ccagaaccgc ctggatcgtg tcgtccttgc cccggctgcg
      601 caccgtcccg taggccacca tcagcaggta gagatcatga ttgcccagca cggtcactgc
      661 ggccggcccc agatcgcgca cgaagcgcaa ggtcttgagg gaatccggcc cccggttcac
      721 cagatcgccg accagccaca ggcggtcgtg gcccgcatcg aactccaccc gcttaagcag
      781 cgcctgcagg gctgaaaagc agccctggat gtcaccgatc gcgtaggtgg ccatggacgc
      841 tcctcaggcg cgctgacgcc cggtgtcaga caccttcgtc agcgggacgg cgtgactcga
      901 cggtgtgtat tccggcaccc agcgccgcag ctcgcgccgg acctctgccg ccggcacggc
      961 ccgacgctgc cgcagccaca atgccagcat gtcgagccag ccggactcga cagcctgggc
     1021 gcgggcgatg cgcagcttgg gatgatgggt cgggcgggtc cgctcggaat cggcgagcac
     1081 ctcttcgtag agcttctcgc ccggacgcaa gccggtgaac acgatgcgga tgtcgtcctc
     1141 agaccgaccg gacaggcgga ccatgtcccg cgccaggtcc tggatgcgca cgggctctcc
     1201 catgtccatc acgaagatct cgcccccctg ccccatggac cctgcctgca gcaccagctg
     1261 tgcggcctca tggatggaca tgaagtaccg cgtgatctcg ggatgcgtca cggtcaccgg
     1321 tccgccggcg gcaatctgcg cctggaactt ggggatcacg ctgccggcac tggccagcac
     1381 attgccaaag cgcacgatct cgaactggga acgcccctcc agagcaaagg cctgacacac
     1441 ctgctcggcc agtcgcttgg tggcgcccat cacattggtg ggattgaccg ccttgtcggt
     1501 ggacaccagc acgaaacgcg gcacaccgaa agccaccgcc gcacgcgcca cctggaaggt
     1561 gcccatcacg ttgttgcgca cggcctgcca ggcgttgtcc tcttccatca ggggcacgtg
     1621 cttgtatgcc gccgcatgga agatcacgtt cggacgatag cggccgatga cttcgtccac
     1681 ccgcaccgca tccttcacgt ccccggccag cgccaccagc tgcagacgcg gaaactcccg
     1741 ggcgaagtcc tcgatcaaac ggtacagggc gaattcgctg agttcgaagc agacaagcac
     1801 ggacggctcg aagcgcgcga tctgccggca cagctccgcg ccgatcgatc cgcccgcgcc
     1861 cgtgaccatg accgccttgc cccgcagata atcctgcacc tgttcggtgt cgatgcgcac
     1921 gggatcccgc ccaaggagat cctccacgtc gagctgacgc acctcactga ggctgtggga
     1981 cgttgagagc cgttcgtcga cacccggaac gatcagggcg gtcaccccga cccggacgca
     2041 ttcgctggca accgcgcgcc gcacctcatg gcgcaccgag ggcatcgcga tgatggcatg
     2101 gccgaccttc agggtttcgg cccagcgttc gagctcggag atggggccga gcaccttgta
     2161 gccgtggatc tcgcgccgct gtttgtgggg gtcatcatcc agcaggccca cgacccgcca
     2221 ctgggccgag gccgccagct ccctcaccag atgggcgccg gccacccccg cgccgaggat
     2281 cagcaccgac tgcccctggg cgcgcagcgc accaaagttg cggaactcct tccaactccg
     2341 gtacagcgcc cgggtgcccc ccatgtacag gagcagcagc accggctgca gcaggaagac
     2401 cagccgcgga atccacggtt cggtccgcac gctcaccacc agcaccaggg gcaccacgac
     2461 agcaccggcg gccaccgcac gcccgatgcg gtgcaaatcg ggcaggctgg cgaagaccca
     2521 gatcccgcga tacagcccga acacccgcag gaacaggcac tggatgggca aggccacggc
     2581 cagaccgtac agcatcgccg gcatgaactc ggccgggatg gccagattga agcggatcag
     2641 gtaggcgacg aaccaggcga gacaaatacc cagagcgtcc gacagaaata ccagaagcgg
     2701 gtgggcgaat ttctggagtg agcgcatcgc agacgggtca tccggaacgg cgccaggcgc
     2761 gatcgatccg gaaccccacg accatataga cgaatgtcca cattgtaata acaaagcatt
     2821 gcagtgcaac acccctatcc atgacaagca acgcgctgcc cccgcaagct gccatcaaac
     2881 catacgcacg cactgcaaca ccgcgatgcc cgtatcccat ctgcaccagc cgctggtaat
     2941 agtgggtgcg gtgggcctgc cacacccgtt cgccacgcaa ggcccggcgg gccagggtca
     3001 ccgacgcatc cacgatgaag ggtgaaaaaa ccagcaccgg aaaccacgcc ggccaacggt
     3061 tccccatcac ccccacgcat cccagggcgc cggccagaaa acccagcggt accgaaccgg
     3121 aatcccccat gaagatccgt gccggcggaa agttgaagca caggaaaccc gccgccgccg
     3181 ccgcaatgga ggcgcacacg gcggccaacg tgccgtcgcc gtgcccacct gccgccaccg
     3241 cataggccac gaagccccat acggccatgc caccggccag tccgtccgat ccgtccatga
     3301 agttatagag attggtcatc cagaccaagc cgagcaccag cgggagcacg cgccaggctt
     3361 ccaccccccc gcctgcgtcc gagatcacca caaagccggc cgccacgccc aggtgcacga
     3421 gcagacgcac ccgcgccgac agatccagcc tgtcgtccgc cagaccgacg accgcgagga
     3481 gcacggcacc cgccagcgcc agcacgagcc ccatggaccc gtgcccggcc agcaacgcca
     3541 gcccgccgcc cagcagcaca cccgccatca cgccgacgcc gccggtccgg ggcgtcggcc
     3601 ggacatgcag cgagcgctcg ttgggctggt cgacaaaatg cagcagggaa tcgggccggc
     3661 agcacgtccg ggtcaccccc caggccgcgc caaacgccag cccgacagcc agcatgtacc
     3721 ctgcctcagc gaccaccgag catctccttc aaaccgtccg ccagcgcaac ccgggcctgc
     3781 cagcccagct ccgtccggat ccgctcggac gagtagcagg ccgacccgag gaggcggtcg
     3841 aggacctcgc cgttgaaggg caggtgcagc cccgccagcc cgccgcaccc atctgccagc
     3901 cacgccgcgg cctgcagcag ccgggccgga accgcccagg cgatcggcgc gagccccatc
     3961 acccggcgca gttcatcgta cagggcccgc ccggaacacg cgatcgggtg ggccaggatg
     4021 taggtccggc cggccgcccg cggatcggac gccaccacgc gtaacgcgct caccacgtca
     4081 tccacgtgga tcacggaacg ccggttgccc gtctccggca gcggcggaaa gcatcctcgc
     4141 cgcacgagac gcgccatccg ctcgagattg ccgcgcccgc cggcgccgta gaccatcacc
     4201 ggcctgagga tcacgacgtg gagtccatgc cggccccccg cctcgaggag ggccgcctcg
     4261 gccgcccgtt tggctgcgcc ataggcaccc tccggtgcgg cggaccacgt ctcgtcggcc
     4321 tgcgcgtcac cgggctcccc ggcggccttg acgctggaca ggaacaccag ccgccgcaca
     4381 cctgcggccg ctgcggcgtc gatcagacgg acgctgccct cgaaattcac cgccctgtga
     4441 cgggcaatct cgtccgcagc atgggtcttg aatgcatggg catggcctgc acagtggaac
     4501 accgtctcca ccccttcgca cgcgcgggcc agcgctgtca cgtcggtcac gtcgccctgc
     4561 acctcggaag gcgcggccgg cgtctggcgg gtgagcaccc gcgggcaggc gccgtcggcc
     4621 tgcagcgcgg tcaccaggcg ccgaccgatg aagccgcgcc cgccggtgac gaggatacgt
     4681 ccgctcaaaa ctccacccgc agacgcagga aactggcaaa acgcggcacg ccgcccggcg
     4741 tcaggcttcg gtagcgatag gtcacccggc tgcccagcgc gggcgggtcg cggcgcacgg
     4801 cgtcgctgaa gccgctgcca aggcgaaagc gcacgccgtc cggcgtctcc acctcaagcg
     4861 cgccaaggcg cccgctgaag cgccccttgc ccggaagatg ggcgatcacc gtggcctcgg
     4921 cgtcaagctg gggcttgagc ttgagcagcg cgtcatgacg gccggtcagg tagggtgcgt
     4981 cgtcccggtg cagcatcagc ccttcaccgc ctgcgcgtac caccgcgtca aggcgggcct
     5041 gcagggccgg ccggtccggc acgcggaact gcgcgacggc ctgcagccag ggcacatcgg
     5101 cgcgggcgac gatctcgcgg atccgggctg cccggtccgt gaaactgccg gcaccgccgg
     5161 gaagctcgaa caccatcagg cgcacacgcc gccactcatc gtcccgcgcg ggttggcggc
     5221 gcaccgcggc cgccagcgca tcgaaggtcc cgcgccccat ccacagttcg ccgtcgaggg
     5281 gctcgggagg cagaccggca atgaaccacg ccggcgccgg cacctcgcga ccgctgcgga
     5341 agcgcagggt ctggccgtcc catatggcgc gggcgccatc aagcttctcg ctgacccagt
     5401 aacgggtcac gtccacatcg ccgtgcagca cctcggccaa taacaggggc gggggcgtcg
     5461 ccgcctgcgc gcgcccgagc atgacgccgg cgagcagcgc caaacctgcc ttgagtccgc
     5521 ggcggcggcg ctgatcaaga tcattcatcg ccatgtccat ccgtgcagtc catagaagcg
     5581 cgccgcgctc tgcatgaaga ggcggacgtg atgccagccc ttgcgcgccg cgtgaccgcc
     5641 atgatgcacc acccggaccg ccggcacata tgcgatccgt gtaatccgcg cgacccgcag
     5701 gctcaggtcg aaatcctcga aatacaggaa gaatcgcgga tcgaagccgc ccacacggcg
     5761 caagacgtcc gcgcgaaaga acatgcaggc cccgctgacg aggggtggat cccacaccac
     5821 ggcatcaccg atcacgtcct gcatctcata gcgcgccagc cgtgcggcaa aacggcgccg
     5881 cagccacgcc ggcgcaaaac cacgcaggaa cagatcgaac accgccggcc ggcgcttgca
     5941 cagatactgg atacggccat cgtcccagcg cgccgcgggg accacgagcc cgcattccgg
     6001 gtgcccgcgc aggaaatcac gcgccgcgcg cagggcgtcc ggcgccattt ccagatcggg
     6061 attgaggatc aggtggatgt cccccgcccc ggccaggctg aggttgtggc cgcagccgaa
     6121 gccgacgttg ccgtgaccgg tgaggacatg cgcggtccag tcggcgggcc agcgcaggga
     6181 cgccatgatc gcggcgccac cgccattgtc gatgaggaaa agctcgggcg tgagcgcggc
     6241 ctcccgacac gcctcgccga gcgcatcgag cgtcgcctgc agcagccggg catccggccg
     6301 gtacagcacg atgctgacgg tcagacggtc catttccacc cgtcgtggtt cggccgcgcc
     6361 gggcgaaccg tcgcccggca tgcttcagac atcagacctt gtagtagctg cgataccact
     6421 cgacgaaacg accgataccc acgtccacgg gcgtcgccgg cacgaagccg gtccacgcgt
     6481 tgagggcgtc ggtgttggcg taggtcgccg gcacgtcgcc atcctgcagg ggcaggaagt
     6541 tcttttcggc ggtcttgccc agcgccttct cgatggcctc gacgaagctc atcagctcca
     6601 ccgggttgtt gttgccgata ttgaacacgc ggaagggcga gttgctggta ccgggatcgg
     6661 gggtgtcggc gtcgtaggcc ggattgggtt ctgccacccg gtcgagggtg cggaccacgc
     6721 cctggacgat gtcgtccacg taggtgaagt cacgcttcat cttgccgtgg ttgaacacgt
     6781 cgatgggccg cccttcgaga atcgccttgg tgaacaggaa cagggccatg tccggacgcc
     6841 cccacgggcc atacaccgtg aagaagcgca gcccggtcgt gggcaggccg aacagatggc
     6901 tgtaggtgtg ggccatcagc tcgttggcct tcttggtggc ggcgtacatg ctcaccgggt
     6961 gatccacact gtcatgctcc gagaagggca tgcgggtatt gccgccgtag acgctggagc
     7021 tgctggcata cacgaggtgc tcgacctgac tgtggcgaca gccttcgagg atgttcatga
     7081 agccgacgag gttgctgtcc acataggcat ggggattgat cagcgaatag cgcacaccgg
     7141 cctgggcggc cagatggatg acgcggtcga acttctcctc ggcgaacagc ttggccatgc
     7201 cgtcccggtc ggccacgtcc atcttcacga agcggaagtt ctcgtggggc gtgaggcgcg
     7261 ccagacggtt gtgcttgagc gcgacctcat agtaatcgtt gaggttgtcg agaccgacca
     7321 cctgatcgcc gcgcgccagc aggagctggc tggtatgcat gccgatgaaa ccggctgcgc
     7381 cggtgacgag aattttcatg gtcgaagata accggacgga ataattcgaa caatcgggcc
     7441 gacatttccg gccgacccgg cgacaggccc gcattctggc acggttgcat tgcagcgtaa
     7501 atcgcacaca ggagtgctcc gtgcggtctg taacgacctt atacttccgc ctgttggcat
     7561 atcgggccag gcagcttgat cctgcgattc ctgtacaccc ttctctggtt gctggctgca
     7621 ccgttcatcg tgctgcgcct ggtctggcgt gcccgtcggc aaccggaata cctccagcac
     7681 ctcggggaac gcctgggccg ctatccccag cgcctgtcgc tgcccgtcat ctggatccat
     7741 gcggtctcgg tcggcgagac ccgcgccagc gccccgctgg ttcaggcgct gctggacgcc
     7801 tacccggatc acagcctcct cctcacccac atgacgccca cgggccgcgc caccggcgag
     7861 gagctcttcg ggcgctttgc gccacgggtt cagagtgctt acctgcccta tgatctgccc
     7921 tgggtcatgc gccgcttcct gcgccacttc cgtcccagca tgggcatcct gatggaaacc
     7981 gaggtgtggc ccaacctggt ggagacctgc cgccgccgcc atattcccct ggccctcgtg
     8041 aacgcgcgca tgtctgaacg ctccgcccgc ggctaccgcc gcgccggccc cctcgcccgc
     8101 gaagccttcg cccacctgag cgtgatcgct gcccagaccg aatccgacga gaaccgcatg
     8161 tccgccctgg gcgcgcgcta tctcaaggtc acgggcaaca tcaagttcga catcatgccg
     8221 ccgaccacga tgcaggcgct cggccacgaa ttccgcaacc gcttcgggcc gcgtcaggtg
     8281 ctcctggccg ccagcacccg cgaaggcgaa gaagaactgc tgctggaaac cttcgcccgt
     8341 ctgtgcccgc cggaagtact gctggtgctg gtcccgcgac atccgcaacg cttcggcgaa
     8401 gtcgtcaagc tcgtgcgcaa ggccggcctg ggcctgcaac gccgctccag caacgatccg
     8461 gtgctggcgg acacgcgggt ctggctgggc gattccatgg gtgagatgtt cgcctactac
     8521 cacgcatcgg acgtggccct catcggcggc tcgtggctgc ccctgggtgg ccagaacctc
     8581 atcgaggcct gcgcggtggg ctgcccggtc gtggtgggcc cccacacctt caacttctcc
     8641 gccgccgccg agcaggccat cgaagccgga gcggcagtac gcgccagcga catggacgac
     8701 ggcatcggcg tcgcgctgca gatcctccag gatcccgccc gccgcagcgc catgggcgcc
     8761 gccggacagg cctacgcgca gacccaccgg ggcgccacgg agcgcaccct cgacctcatc
     8821 acgccgctgc tgcgggctgc acgaccgcgc cgttgaggcg ccccagcgcg gcttgacggg
     8881 cgtcctccgc gcgagccgtc agcccacggg tttgagcaga tagtccagcg cccagccggc
     8941 gaagatcgcc ccacccaccc agttgttgtg caggaaggcc ctgaagcagt tcgcccggtc
     9001 ccgcccgcgg atcagggtga agtgatagcc catcagtccc gccgccaccg ccagtcccgc
     9061 cagaaaggcc gcaccccgcc cggtgtggag gccgacccag gccagcaggc ccagcgtcac
     9121 ggcgtagcac agcatcacgg ccgccacgtc gaagcggccg aaggtgatcg ccgaggtctt
     9181 gatgccgatc ttcagatcat ccgggcgatc caccatggcg tactccgtgt cgtaggccac
     9241 cgcccagaac acgttggcca gcaacatcac ccaggcctcc acaggcaccg cgtccaggca
     9301 ggcggcaaag gccatcggga tgccgaaacc gaaggcgatg cccagatagg cctggggaat
     9361 cgccaggaag cgcttggtga aggggtagct gcctgccaga aacaccgccg gcacgctgag
     9421 cagcagggtc agcgtgttga ggggcagcac gagcaggaag gacaccagcg ccagcaccgc
     9481 cgccagcacc agcgcctcgg ccggacgtac ccgcccggcc gccagcgggc ggttgcgcgt
     9541 gcgctccacg tggccgtcga aattgcggtc ggcgtaatcg ttgatgacgc agccggcgga
     9601 gcgcatcagc accgtgccga gcacgaagat ccacacgatg aaaggggacg gccgtccgcc
     9661 cccggccacc cacagcgccc acagggtcgg ccacagcagc agcaggatgc cgatgggctt
     9721 gtcgaggcgc atcagctttt cgtattcgga caggcgttcg gcaaaggtca tggggccagg
     9781 ctccggatcg cgggcaggaa gacttcggtg atcagcagcg gcgaaccggc ccgccggaag
     9841 gccgagcggc gcgcccacag ggctggcaga tcgctccgcc cggtggccgc ggcggcccgg
     9901 tgccaacggg tgtcgcgggg atcgagacgg gccgcctcca ggggcgagcg acgtacccgc
     9961 gggtcgtcga agagcaccgc ggccaggggc cgtccgccca gatgacggag caggtgccag
    10021 gccgccggca cggtgctggc ggccaccagg gagcgggcga acaccaccgg cgccccgtcg
    10081 gccagcagga gcacctcccg ctgccacagc tcaccgtggc gatgcacgcc cagcgcggcc
    10141 agctcgtccg gcaagggccg gcccttgccc tgggacacca ggtgcaggga gaaggtgccg
    10201 cagcgtgcac ggatgcgcgc ggtgagcgaa ctgtcgtcgc tgagccagcc ccgcaagggt
    10261 cggggagcgc ggctggcggt catgcggaag ggatcgcggc ccggaaagtg ggcaagggtc
    10321 atggagcggc ggagcacggg gtcaggcggt ggggataacg gcggcagcgg cgccattgta
    10381 gggcgtttgc cggcgcccga gaatggccgc cacgcgggcg tcggcccgcc gtcctcacgc
    10441 cttgagcagc gcggcgcgac cgccgaacca gcgggcggtg atcgcatccg ccaccgccgg
    10501 cgcgtcccgc agcagggttt ccgccaggtc ccgcgccacc gccaccagat ccgcatcggc
    10561 ctccaggtcc gcgtggcgca gcagcggcac gccgctctgg cgggcaccga tgaactcgcc
    10621 cggaccgcgg atgcgcaagt cttcgcgggc gatgacgaag ccgtccgcat gttcgtagat
    10681 ggccttgaga cgctcccgcc cggtctggga caggggctgg gcgtaaagca ggatgcacgc
    10741 cgaggcatgg gcgccacgcc cgacccgccc gcgcagctgg tgcagctggg ccaggccgaa
    10801 gcgctcggca tgctcgatca ccatcaggct ggcgttgggc acatccacgc ccacctcgat
    10861 caccgtggtg gccaccagca cgtggatctc gcccgccaca aaggccgcca tggtggcgga
    10921 tttctcgtcg ttccggaagc ggccgtggac cagcccgacc ttcaggtccg gcagcgccgc
    10981 ggcgagcagg gcgaaactct cttcggcggt cttgagctgc agcgcctccg actcctcgat
    11041 cagcggacac acccaatagg cctggcgccc cgccacgcag gcatcgtgca cgcggcggat
    11101 cacctcgtcc cggcgcgcct cggccaccag cttggtgacg atgggcgtgc ggccgggcgg
    11161 cagctcatcc accacggaga catccaggtc agcgtaatag ctcatggcca gggtgcgcgg
    11221 gatgggcgtg gcggacatca tcagcatgtg gggcgcaccg ccacgacctt tctcgcgcag
    11281 cgccaggcgc tgacgcacgc cgaagcggtg ctgctcgtcc accaccgcca gggccagacc
    11341 gggcaggctc accgggtctt cgatgagggc gtgggtgccc accgccagca gcagttcgcc
    11401 ggccgccagg cgagcgatca gggcctcgcg ctcgcgcttg cgacgactgc ccgacagcca
    11461 ggccacctcc agcccgagag gcgcgagcca gccggcgagt ttgcggtagt gctgctcggc
    11521 gaggatctcg gtgggcgcca tgagcaccgc ctgccgaccg ttctccgccg cccgcagcat
    11581 ggccagcgcg gccacgcagg tcttgccgct gcccacatcg ccctggagca ggcgctgcat
    11641 gggatgtgcg gccgccaggt cgcgggcgat ctcgtcccag gcgcgacgct gggcgccggt
    11701 gaggctgaag ggtagctccg tcagcagggc gcgggtcagg gtatcgacgg ccggcagcgg
    11761 cggggcgttc ttggcacgcc gcgcggcgta ggcgcggcgc agggagatct gctgggcgag
    11821 gagttcgtcg agcttgatgc gctgccaggc ggggtggtgg cggtcttcca gctccactgc
    11881 ggcgagcgac ggcggcggcg cgtggagcag gtgcagggcg tccggcaggg ccatcaggtc
    11941 gaggcgccgg accacctcgt ccggcagatc ctcgcggggc ggatgatggc gcatggcgcc
    12001 accgatgagc ttgcgcaggg ccgactgggc cagcccggcg gtggtcggat agaccggcgt
    12061 gaggctctcc ggcaaggact cgccctcggc cacggggcgc acgcgggggt ggatcatctc
    12121 ggcgccgaag aagcccggac ggacttcgcc gaacacccgc aggcgcgccc ccggcgccag
    12181 ctgcttctgc tggctggggt agaaatgcac gaagcgcaga ctcacctcgc cgctggcatc
    12241 cgccgcccgc gccacgagct gacgccgcgg acgcagttgc acctcgcagt gcagcaccgt
    12301 cacctcgcac tgggcggcgc agccgggccg cacgtcggcc agcatcgtga cgcgggtctc
    12361 gtcctcgtag cgcaggggca ggtggaggat caggtcctgt ggcccgaaga tgtccagcct
    12421 ggccagacgg ccggccagtt gcggaccgac gcccggccag ccccgcggcc cggggcccgt
    12481 ttcgggcggc ggggcgctca ccggtggctc agcccagcac gagcacggcg tccgcctcga
    12541 cgagggcgcc gcggggcagc tccttcacgc cgacggccgc gcgggcgggg aagggctcgc
    12601 tgaagtagcg ggccatcacc tcgttcacct tggcgaaatg ggccagatcg gtgaggtaga
    12661 ggttgagctt gaccacgtcg gcgagggacg caccggccgc ctcggccacc gccttcaggt
    12721 tctcgaagac acgcacggtc tgggcctcaa agccgtccat cagctgcatc gtggccgggt
    12781 ccaggccgat ctggcccgac aggtagacgg tgtcgccggc gcgcacggcc tgggaatagg
    12841 taccgatggc ggcgggggcg tggggtgtgg cgatgatctg cttgggcatg ggggtgggct
    12901 ccgaggggtc aacacggccg cgtaagcgat cacgcggcac gcaaagccgc gagtgtacgg
    12961 tgcggccccg cccgtcggga agcccgctcc cgcacgaggc cccggcgcgc acctccgcac
    13021 agcgcctgaa accgccggtc cgacacgccc gcaggcccgt tcgtccccag gcgcacgtcg
    13081 ttgcgatgcc ttacgggaag ggcccgacga tgccctgacc tatgactttc gtcatatcag
    13141 acgatcaagt cgggctgcat gctgccgtta gtcgacgacg tactcacccg aaatgatctt
    13201 cccccatgat ccggacccgc ttccgcgctg acgacttcgc catactcgtc gccctcgcca
    13261 ccagcgcggg cggcattgcc tcggccgcgc tcggcggccc cgcgggcctg tccctggttg
    13321 ccggcacctt cacggtggtg gccctggtca cggccatgat cgtcagccgg cgcgcccgcg
    13381 aacgctggcg tcagcaacag caggcccagc gggagtcgat cgacagcagc gtgcgcgaat
    13441 acgacatgct gtgcgatcag atcgcggtcc agagcggctt ccagtacgaa cgcctgcggg
    13501 aatccctggg gcagatgcag gacgtggtga gcagcgcagc cagcaagctc ggtggcagcc
    13561 tcgccggcgg ccaggggggc aattcccgcg accagctgcg ccatctggtg gacgaactgc
    13621 tgtccctcgc cgccgaggaa gagcgcgccc tgcaggaatc cgggctggag cgcttcgccg
    13681 cggagacccg cgacgccttg cgcgacttcg tcggtaccgt ggagcgcctc aaggccagcg
    13741 gcagcgacat cgccgagcgc ttcggcgcaa tgcgcggcaa ggtggatgcg gtcacgcgcc
    13801 tgatgaccga ggtcaatcag atcaacagcc agaccgaact gctcgccctc aacgccgcca
    13861 tcgaggcggc ccgcgcgggt gaggcggggc gcggctttgc ggtcgtcgcc gacgaagtgc
    13921 gcaaactggc ccagcgcacc gagaccttca gcgatcagat cgcgaccctc ctcacggaca
    13981 tccacgcctc catcgacgac gtgggccgcg ccgtggatgt cacggccgcc accgacgtga
    14041 gcaaggcgcg ctcatccgaa gcgagcgtgt ccgcaatggg gctggagatg agcgaactga
    14101 acgcccgcgc catggttcag tcgcgccgca tcagcgaact ctccgccaag atccatcgcc
    14161 tggtgatgga aggcatccag tccatgcagt tcgaggacat cgtccgccag atcctcgaca
    14221 agctgcgcca gcacaccgac ttcatggggc gttacgccca cggcttcttc gacgcccacc
    14281 gggatcccga acagcgcgag gcgacctccc gcatccagcg ccgcgtggag atcctgcgcc
    14341 agctgctggc ccagtccgag cagtcgaaga aggacatccg cttcgaggcg gtccagcaga
    14401 ccgccgtgca tgcgggcgag gtggatctgt tctgacgccg
//
LOCUS       DAOQMM010000097        14194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1766_length_14194_cov_49.493387, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000097 DAOQMM010000000
VERSION     DAOQMM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_14194_cov_49.493387"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="PLH85_13805"
     CDS             <1..1525
                     /locus_tag="PLH85_13805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB65687.1"
                     /translation="RLIVDPLTCAGYAALGVAPRLIVDPLTCAGYAALGVAAGALGAW
                     WPAREAARVAPAQALKAAADEALPGGSGRPWAALTLWCLAAAACLLPPIGALPIGGYG
                     AVAGILAGAILFLPTIARVLSRCLPRPRSVAGRLAVARLVAVPGHVVVAAAGVLTSVA
                     LAAAMAIMVTSFRESVDAWLTQLLPADIYVRAGRAGAAGVLDEALQARLAALKGVADV
                     RFTRHEQLRLDGHGAPVALIARPVSADGAGLPRVGGPRVPSGAPGLWISEAMADLYGW
                     QPGMTVTLPLAGRAVTLPVAGVWRDYARQTGSVLLDLEAYRRFTGDRRVHDAALTLAP
                     GASAPDVVRAVAALAPEGSLEVSRPGEIRAITLGIFDRTFAVTYLLEAVAVLIGLAGV
                     AASFAALAAARRREFGMLRHLGVSRREIAAMLAREGALAAGVGVLGGLLAGGAIALVL
                     IEVVNRQSFHWSMDLHVPWGSLAVFALTLVALAALAAVAAGRQAMRREAVWAVREEG"
     gene            1525..2631
                     /locus_tag="PLH85_13810"
     CDS             1525..2631
                     /locus_tag="PLH85_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNB65688.1"
                     /translation="MQRRWFLGAVPGLAIAWAGRARAGADGFARVVPGRRLSFPADHG
                     AHPAFRTEWWYVTGWLTLPDGQPLGFQTTFFRVRTGIGEDNPSAFAPQQLVLAHGALA
                     DPRLGHLRHAERVARMGFGRAGFETSACRVWVGDWRFELDGGRYRARVRGEGFAYALD
                     FEPDGAPVLNGVAGFSAKAADPRHASYYYSRPQLAVTGQVTREGVAQRVKGLAWLDHE
                     WSSELLPTGAQGWDWIGLNFNDGTSLMAFRLRASRGEPLGASATLRTRTGVHTVPATA
                     VAFEPLRTWTSSRTGIRYPVAWRVRVADRLIELQPLLDDQELDSRRSTGAVYWEGAVR
                     ALEGGRETGRGYLEMTGYGDAIRLGARGEEDGSA"
     gene            2759..3100
                     /locus_tag="PLH85_13815"
     CDS             2759..3100
                     /locus_tag="PLH85_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNB65689.1"
                     /translation="MGERYRVHIENSGEDYVCPTEQSLLRGMEVLGRRGIPVGCRGGG
                     CGVCKVHITAGDYEARKMSRACVSEDEERHGVVLACRVYPRGEISLEVIGKMARAIGT
                     AAPGPGDVSGV"
     gene            3324..4934
                     /locus_tag="PLH85_13820"
     CDS             3324..4934
                     /locus_tag="PLH85_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNB65690.1"
                     /translation="MNSSAEAAVPSGAAIRPNLDRPSGLVTRYVFLAVMAAGLLFTGY
                     SVFADIAESGAVVTTLLPFVLLGVALLIALGFEFVNGFHDTANAVATVIYTHSLPPHV
                     AVVWSGVFNFIGVLVSSGAVAFGIISLLPVELILQVGSSAGFAMVFALLIAAILWNLG
                     TWWLGLPASSSHTLIGSIIGVGVANALMHGRDGTSGVDWAQATKVGYSLLLSPLVGFG
                     CAALLLLALRALVKNRALYEEPRSDAPPPWWIRGILILTCTGVSFAHGSNDGQKGMGL
                     IMLILVGTVPLSYALNRAMPASQVTQFAAVAHVTQDALVKAAPVAPPAEPRKVLSDYI
                     RTHEVTPEVVPALAALTGTIGAQVKDYPSLARVPAESVPNVRNDMYLASEAIRFLEKS
                     DQVNFDADAKANLKAFKGQLDTATKFIPLWVKVAVAVALGLGTMIGWKRIVVTVGEKI
                     GKSHLTYAQGASAELVAMTTIGAADLFGLPVSTTHVLSSGVAGTMAANKSGLQMATIR
                     NLLMAWVLTLPAAILLSGSLYWLFAHLF"
     gene            4955..5890
                     /gene="ppk2"
                     /locus_tag="PLH85_13825"
     CDS             4955..5890
                     /gene="ppk2"
                     /locus_tag="PLH85_13825"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNB65691.1"
                     /translation="MSTSHPVDQEELLRRLQRDLIDGYDEELEMEIEDRPWTDPMQPI
                     AVDEAEAEAHRQQRHRYFQDLFRLQGELVKLQDWIATTGQRAVILFEGRDAAGKGGVI
                     KRVVQRLNPRVCRTVALPAPSDRERTQWYFQRYVAHLPAAGEMVLFDRSWYNRAGVEK
                     VMGFCSDDEYEEFFRSVPEFERMLVRSGIKIVKYWFSITDDEQQLRFLSRIHDPLKQW
                     KLSPMDMESRRRWEDYTRAKEIMLERTHIPEAPWWVVQAVDKKRARLNCIHHLLQQFP
                     YEEIQRPPVLLPARERHEDYQRSAVQSDLFVPEIY"
     gene            5943..6959
                     /locus_tag="PLH85_13830"
     CDS             5943..6959
                     /locus_tag="PLH85_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNB65692.1"
                     /translation="MSSAALTAYGADPSGLVYGYVFTADGVGRQVDTAAAGAWLAERD
                     ARPGEFIWLHLTGVQAATMNWLQSHLELSEDFTDALKEGSRSTRIELVHASLIAVIND
                     VVYDLSRENTLQVATLWMNVTPRCLVSVRIQPLRSVDRLRAAVKAGETFGSPLALVIH
                     LLREQADMLVHIIRSATEKVDGVEDKFLLGRLPERAALGGIRRDLVRLQRLLAPEPAA
                     LFRLLNRPPAWVSAGDTQDLQQSTEEFSVVLRDMAGLQERIKLLQEEIVAHIGERTNR
                     SVFVLTAVTVIALPINLTAGLFGMNVGGIPLAEDPEGFWRVLLLVLLMTAGAAWYAFR
                     RGPE"
     gene            complement(7035..8717)
                     /locus_tag="PLH85_13835"
     CDS             complement(7035..8717)
                     /locus_tag="PLH85_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB65693.1"
                     /translation="MTIKYPENKDLRELIRFSADDGLIWLGESRMLLLQVTTMQALRK
                     ELIHSVGEDQARRVLTRMGFGAGMRDAELARKVRGSRDMLDAFCVGPQLHMLEGSVQV
                     EPIRLDMNPDTGSFYGELLWKNSWEVDAHVREFGPADHPVCWMQIGYASGYSSCFMGR
                     FILFREVECAAAGANTCRIVGKPAEEWPDAAELLRYYDDTAVVGRILELRDEVDTLRS
                     TLGASHQIPSLIGASASFQHAYTLIKKAAPTQVTVLLLGETGVGKERFARALHELGSR
                     RDAPFVAVNCAALPHDLIESELFGVEKGAFTGAHASRAGKFERADGGTLFLDEVGEMP
                     LAAQAKLLRVLQDGEVERLGDDRPRKINIRLVAATNVDLQAAVRDGRFRSDLYYRLNV
                     YPVVIPPLRERVSDIPPLAHAMLERFCAVHAKRLKGVTDKALRALKAHKWPGNVRELE
                     NMMERGVILAAHDGWIEAEHLFAGLDISPCSEDRVDASGGLRPATADAAPDVSALCDA
                     VFASGISLDDLEAALLREAMQRQQGNLSGAARLLGLTRPQFSYRLKREKPLA"
     gene            9309..9566
                     /locus_tag="PLH85_13840"
     CDS             9309..9566
                     /locus_tag="PLH85_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit"
                     /protein_id="HNB65694.1"
                     /translation="MKSDTPAVDVNRKFVRLVERRANGLVEFEFSIGDPELFVEMLLP
                     ATAFEEFCAVNRVEILEGSRPHEAGSDWDWSLHEATHQRFR"
     gene            9613..10611
                     /locus_tag="PLH85_13845"
     CDS             9613..10611
                     /locus_tag="PLH85_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HNB65695.1"
                     /translation="MQIDLRTVAINPLRQTYDHIARRIGPDKAASRYQEGTLDLQATH
                     NFHYRPTWDPDHEIFDASRSAIRMADWYAFKDPRQFYYGAYTLARAKQQDTAEANFDF
                     VENRGLAATLPEDLRATVLAVLLPLRHVAWGGNMNGAFIAAYGYGTALTQPSLYHAMD
                     QLGIAQYLTRVGLLLGDDPSVLDDAKTRWMEDGVWQGLRRYVEDTFVLKDPFELFVAQ
                     MVALDGLLYPLVYETIVDDVLSANGGTAVAMLTQFMTEWFAETRKWVDATVKAAAAES
                     PENKALLEKWIGHWRDRAAAALMPVAQIALGSQADAVTSELVSQFNTRLAKAGVSL"
     gene            10623..10892
                     /locus_tag="PLH85_13850"
     CDS             10623..10892
                     /locus_tag="PLH85_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HNB65696.1"
                     /translation="MSKVFIALQTNDETRQIIDAILADNPHAIAVNQPAMVKIDAEGS
                     LVVRRSTIEELLGRDYDLQELHINLISLTGHVDETDEELTLSWNA"
     gene            10912..12465
                     /locus_tag="PLH85_13855"
     CDS             10912..12465
                     /locus_tag="PLH85_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene/methane monooxygenase
                     hydroxylase/oxygenase subunit alpha"
                     /protein_id="HNB65697.1"
                     /translation="MDMKVSKKKLGLKEKYQHMTRGLGWETSYQQMDDVFPYAKYEGI
                     KIHDWDKWEDPFRLTMDAYWKYQAEKERKLYAIIDAYAQNNGHLSVTDARYINAIKLF
                     LNGISPLEYMAHRGFAHVGRQFPGVGPRVACLMQSLDEIRHAQTQIHALSNYNKYYNG
                     FHEFRHMIERVWYLSVPRSFFDDAVTAGPFEFMVAIGFSFEYVLTNLLFVPFVSGAAY
                     NGDMGTMTFGFSAQSDEARHMTLGLEVIKFILEQDPDNLPIVQRWIDKWTWRGYRVLT
                     LVGMMMDYMLPKRIMSWKEAWEVYFEENGGALFKDLARYGIRPPKWLDQIAFDKDHLS
                     HQAWATFYQYGAVCNFHTWMPTDDEMDWLSKKYPNSFDKYYRPRLEFWRDEAAKGNRF
                     YNKTLPMLCQTCQIPMLFTEPGDPTQICYRQVDYKDNTYHFCSDGCKEIFEHEPEKYI
                     QAWLPAQQIYQGHCFNEGVDPTAEGFDPLGAVLDYYHMAAGRDNFDFDGSEDQKNFEA
                     WRGLATKNA"
     gene            12537..12896
                     /locus_tag="PLH85_13860"
     CDS             12537..12896
                     /locus_tag="PLH85_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit P4"
                     /protein_id="HNB65698.1"
                     /translation="MGVAAIKDYTFEPADRLENFHGNQLLNIGWDQHLLFCAPFCLPL
                     PPTTRFGDIVDKILPGIFGYHPDFAKIDWSTVQWLKGGQPWQPDFARTLAELGLRHKD
                     ALRFRTPGLNGIQGSAS"
     gene            12899..13954
                     /locus_tag="PLH85_13865"
     CDS             12899..13954
                     /locus_tag="PLH85_13865"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenol 2-monooxygenase domain-containing
                     protein"
                     /protein_id="HNB65699.1"
                     /translation="MGYELTIEPLGQTIEVEEGQTILDASLRAGIWLPHACCHGLCAT
                     CKVQVVDGEVDHGEASSFALMDFERTEGKTLACCARLESDVTIEAEIDEEPDAESIPV
                     KDFSGTVSRIETLTPTIKGLFFTLNEPLHFQAGQYVNLEIPTLGISRAFSLANVPSTG
                     REVELNVRIVPGGAGTGYLHERLKVGDPIRLSGPYGRFFVRKSAQVPLIFMAGGSGLS
                     SPRSMILDLLAEGSTLPITLVYGQRRREELYYHDEFVELAARFPNFSYVPALSDEPAD
                     SGWTGHRGFVHEAAKAHFDNDFRGHKAYLCGPPAMIDACISTLMQGRLFENDIYTEKF
                     FSAADAQQVRSPLFRRV"
     gene            14006..>14194
                     /locus_tag="PLH85_13870"
     CDS             14006..>14194
                     /locus_tag="PLH85_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65700.1"
                     /translation="MSRSCIKLATSSAEPTGGHQAPSAEDSQKEEETIMAMTGVLRPG
                     HMQLRVLDLEEGVNHYKNV"
BASE COUNT         2286 a         4594 c         4918 g         2396 t
ORIGIN      
        1 acggctgatc gtcgatccgc tgacctgcgc aggctatgcg gccctgggcg tggcgccacg
       61 gctgatcgtc gatccgctga cctgcgcagg ctatgcggcc ctgggcgtgg cggccggggc
      121 gctgggcgcg tggtggcccg cgcgggaggc tgcccgggtg gcgccggccc aggcgctcaa
      181 ggccgcggcg gacgaggcat tgcccggcgg cagcggtcgg ccgtgggcgg cgctgacgct
      241 gtggtgtctg gcggcagcgg cctgcctgct cccgcccatc ggggcgctgc ccatcggggg
      301 gtacggggcc gtggccggca tcctggcggg ggcgatcctg ttcctgccga ccatcgcccg
      361 cgtcctctcc cgttgtctgc cgcggccgcg gagcgtggcg ggacgcctcg ccgtcgcccg
      421 tctggtggcg gtgccgggcc atgtggtggt ggccgccgct ggcgtgctca ccagcgtggc
      481 gctggcggcg gccatggcga tcatggtgac ctcctttcgt gagtcggtgg acgcatggct
      541 gacccagctg ctgccagccg acatctacgt gcgggccggc cgggccggcg cggcgggtgt
      601 cctcgacgag gcgctgcagg cgcggctggc agcgctgaag ggcgtggcgg acgtgcgctt
      661 cacgcgtcac gagcagctgc gcctggacgg gcacggcgca ccggtggcgc tgatcgcccg
      721 gccggtgtcg gcggacggcg ccggcttgcc gcgggtcggg ggcccgcgtg tcccgtccgg
      781 cgcgccgggc ctgtggatct cggaggccat ggcggatctg tatggctggc agccgggcat
      841 gacggtgacg ctgcccctgg ccgggcgggc cgtgaccttg ccggtggccg gcgtgtggcg
      901 ggattacgcg cgccagaccg ggtccgtgct gctggatctg gaggcctacc ggcgcttcac
      961 cggtgaccgc cgggtccatg acgcggcgct caccctggcg cccggcgcct cggcaccgga
     1021 tgtggtgcgg gcggtggcgg cgctggcgcc ggaggggagc ctggaggtga gccggccggg
     1081 cgagatccgg gcgatcaccc tgggcatctt cgaccggacc tttgccgtta cctacctgct
     1141 ggaggcggtg gcggtgctca tcggcctggc gggcgtggcg gcgagctttg cggccctggc
     1201 ggcggcccgc cggcgggaat tcgggatgct ccgccacctg ggagtgtcac gtcgtgagat
     1261 tgccgcgatg ctggcgcggg aaggggcgct ggcggccgga gtgggggtgt tggggggact
     1321 gctcgccggc ggcgccatcg cgctggtgct gatcgaggtg gtgaatcggc aaagttttca
     1381 ctggagcatg gatctgcacg tgccgtgggg gtcgctggcc gtgttcgcgc tcaccctggt
     1441 ggccctggct gcgctcgccg cggtggccgc cggccgccag gcgatgcgcc gggaggcggt
     1501 gtgggcggtg cgggaggagg gctgatgcag cggcgatggt ttctcggggc ggtgccgggc
     1561 ctggcgatcg cgtgggcagg gcgggcgcgg gcaggggcgg acggattcgc gcgggtggtg
     1621 ccggggcggc gcctgagttt tccggcggat catggcgccc acccggcgtt tcgtacggag
     1681 tggtggtatg tgaccggatg gctgacactg ccggacgggc agccgctggg cttccagacc
     1741 accttcttcc gggtccggac cgggatcggg gaagacaacc ccagcgcctt cgcgccgcag
     1801 cagctggtcc tggcccatgg ggcgcttgcc gatccgcggc tgggacatct gcgtcatgcg
     1861 gaacgggtgg cgcggatggg ctttggccgg gctggcttcg agacgtccgc ctgccgggtc
     1921 tgggtgggcg actggcggtt cgagctggac ggcgggcgct accgggcccg cgtgcgggga
     1981 gagggctttg cctacgcgct ggatttcgag ccggacgggg cgcccgtgct caatggcgtg
     2041 gcgggcttca gcgccaaggc cgcggatccg cgtcacgcca gctattacta cagccgcccg
     2101 cagctggcgg tcaccgggca ggtgacgcgc gagggtgtgg cgcaacgcgt caaggggctg
     2161 gcgtggctcg atcacgagtg gtccagcgaa ctcctgccga ccggggccca gggctgggac
     2221 tggatcggct tgaattttaa cgacggcaca tccttgatgg ccttccgcct gcgcgcctca
     2281 cgaggcgagc ccctgggcgc gtcggccacg ctgcggacac gcacgggcgt gcacaccgtg
     2341 ccggcgacgg ccgtggcctt tgagcctctg cgcacctgga cctcctcccg taccggcatc
     2401 cgttacccgg tggcctggcg cgtgcgggtt gcggaccggt tgatcgagct gcagcccctg
     2461 ctggatgacc aggagctgga cagtcgccgc tccaccggcg cggtgtattg ggaaggtgcc
     2521 gtgcgggcgc tggaaggcgg ccgggagacg ggacggggtt acctggagat gacgggctac
     2581 ggcgatgcga tccggctggg tgcgcggggg gaggaggacg ggtccgcgtg accggtctgg
     2641 atgagagccg gcgccaaaat aaatttgcct gattggtcta ttcgcgataa taattagaaa
     2701 tctcgagaaa aatacgggcg ccaggatggg tgacaccgtt gcacacgagg aggagaccat
     2761 gggtgaacgt tatcgcgtcc acatcgaaaa cagcggggag gactacgtct gccccaccga
     2821 acagagcctc ttgcgcggca tggaagtgct gggccggcgc gggattcccg tcggctgtcg
     2881 gggcggcggt tgtggggtgt gcaaggtgca cattaccgcc ggcgattacg aggcgcgcaa
     2941 gatgagtcgt gcctgcgtca gcgaggatga agagcgtcac ggcgtggtgc tggcctgccg
     3001 ggtctatccc cgcggcgaga tttccctgga agtgatcggc aagatggccc gcgcgatagg
     3061 gacggcggcg cctggtccgg gcgacgtgtc cggggtctga gtctttcagc cgggagcccc
     3121 gcccccgtca tctcacctcc ctctcccgac ggggatctcc acggcttttc ctcggccgtc
     3181 gcgcaatgcg acggccgatt tttttggcgc cggacgcgct ggcagaggcc ggcggccgtc
     3241 tgggcgcggt gcggtggcaa tcatacgggc gtcacgcagg gcttccagaa tgggcgccat
     3301 tccaactgtc ggtggggttg cccatgaatt cctcagcaga agcggccgtg ccttccggcg
     3361 cggcgatccg tcccaatctg gaccggcctt cgggactggt cacccgctat gtcttcctgg
     3421 ccgtgatggc agccggcctg ctgttcaccg gctacagcgt gtttgccgac attgccgaga
     3481 gcggcgcggt ggtcacgacc ctgttgccct tcgtgctcct cggcgtggca ctgctcattg
     3541 ccctcggctt cgagttcgtc aatggtttcc atgacaccgc caatgcggtg gccacggtga
     3601 tctacaccca ttccctgccg ccccacgtgg cggtggtgtg gtccggcgtg ttcaacttca
     3661 tcggcgtgct ggtgtccagt ggcgcggtgg ccttcgggat catctccctg ttgccggtgg
     3721 agctgatcct gcaggtgggc tccagcgccg gcttcgcgat ggtgttcgcg ctgctcatcg
     3781 cggcgatcct gtggaacctg ggcacctggt ggctggggct gccggcgtcc tcgtcccaca
     3841 ccctgatcgg ctccatcatc ggcgtcggtg tggccaatgc gctgatgcac gggcgtgacg
     3901 gtaccagcgg cgtggattgg gcgcaggcca ccaaggtggg ctattccctc ctgctgtcgc
     3961 cgctggtcgg cttcggttgt gcggctttgc tgctgctggc cctgcgggcc ctggtgaaga
     4021 accgggcgct ctacgaggag cccaggagcg acgcgccgcc gccctggtgg atccgcggca
     4081 tcctgatcct gacctgcacc ggtgtgtcct ttgcccatgg gtccaacgac ggccagaagg
     4141 gcatgggcct gatcatgctg atcctggtgg gcaccgtgcc cctgtcctac gccctcaacc
     4201 gggcaatgcc ggcgagtcag gtgacccagt tcgccgcggt ggcccacgtc acccaggacg
     4261 ccctggtcaa ggctgcgccg gtggcgccgc cggccgagcc gcgcaaggtg ctgtcggact
     4321 acatccggac ccatgaggtg acaccggagg tggtgccggc cctcgccgcg ctgaccggca
     4381 ccatcggtgc gcaggtgaag gattatccgt ccctggccag ggtgccggcg gaatccgtgc
     4441 ccaacgtgcg caacgacatg tacctggcgt cggaggcgat ccgctttctt gaaaagagcg
     4501 accaggtgaa tttcgacgcc gacgccaagg ccaacctgaa ggccttcaag ggccagctcg
     4561 atacggcgac caagttcatt cccctgtggg tgaaggtggc ggtggccgtg gcgctgggcc
     4621 tgggcaccat gatcggctgg aagcgcatcg tcgtgaccgt cggcgagaag atcggcaagt
     4681 cgcacctgac ctacgcccag ggcgcgtccg ccgagctggt ggcgatgacc accatcggtg
     4741 ccgccgacct gttcggcctg ccggtgtcca ccacccacgt gctgtcatcc ggcgtggcag
     4801 gcaccatggc cgccaacaag tccggcctgc agatggccac catccgcaac ctgctgatgg
     4861 cctgggtgct gaccctgccg gcggcgatcc tcctgtcggg cagcctgtac tggctgttcg
     4921 cccacctgtt ctgagccgta cgaggaatga aagcatgagc acatcgcacc cggtcgatca
     4981 ggaggaactg ttgcgccgtc tgcagcgcga cctcatcgat ggctacgacg aagaactgga
     5041 gatggagatc gaggaccggc cgtggaccga tccaatgcag ccgattgccg tcgatgaagc
     5101 cgaggccgag gcccaccgcc agcagcgcca ccgctacttt caggatctgt tccgcctgca
     5161 gggtgagctg gtcaagctgc aggactggat cgccaccacc ggccagcgtg ccgtgatcct
     5221 cttcgagggg cgcgatgcgg cgggcaaggg gggcgtcatc aagcgcgtgg tgcagcgcct
     5281 caacccgcgg gtgtgccgca ccgtggcgct gccggcgccg tccgaccggg agcgcaccca
     5341 gtggtatttc cagcgctacg tggcccatct gccggcggcc ggtgagatgg tcctcttcga
     5401 ccgcagctgg tacaaccggg ctggcgtgga gaaggtgatg ggcttctgct cggacgacga
     5461 gtacgaggag ttcttccgct cggtgccgga attcgagcgc atgctggtgc gctccggcat
     5521 caagattgtg aagtactggt tctctatcac cgacgacgag cagcagctgc gcttcctgag
     5581 ccgcatccac gatcccctca agcaatggaa gctgagcccc atggacatgg aatcgcggcg
     5641 tcgctgggag gattacaccc gcgccaagga gatcatgctg gagcgcaccc atatccccga
     5701 ggcaccctgg tgggtggtcc aggcggtgga caagaagcgg gcgcgcctga actgcatcca
     5761 ccatctgctg cagcagttcc cctacgagga gatccagcgc ccgccggtgc tcctgccggc
     5821 gcgggaacgc catgaggact accagcgcag cgccgtccag tcggacctgt tcgtgccgga
     5881 gatctattga gcgtgatgga cgacgacgat gccccggtac aacggtgggg cacggggcgc
     5941 cgatgagttc ggccgccctc accgcctatg gcgcggaccc ttcggggctg gtgtatggct
     6001 acgtgttcac ggcggacggc gtcggccgcc aggtggacac ggcggcggcg ggcgcctggc
     6061 tggccgagcg ggatgcccgt ccgggcgaat tcatctggtt gcacctgacc ggtgtacagg
     6121 ccgccacgat gaattggctg cagagccacc tggaactgtc ggaggatttc accgatgccc
     6181 tcaaggaagg ctcccgcagc acgcgcatcg agctggtcca tgccagcctg atcgccgtca
     6241 tcaacgacgt ggtctatgac ctgagccgcg agaacaccct gcaggtggcg accctgtgga
     6301 tgaacgtgac gccccgctgc ctggtgagcg tgcgcatcca gcccctgcgc tcggtggatc
     6361 ggctgcgcgc cgcggtgaag gcgggcgaga ccttcggcag cccgctggcg ctggtgatcc
     6421 acctgctgcg ggaacaggcc gacatgctgg tgcacatcat ccgcagcgcc accgagaagg
     6481 tggacggggt ggaggacaag ttcctcctcg ggcgcctgcc cgagcgggca gcgctgggcg
     6541 gcatccgtcg cgacctggtg cgcctgcagc gtctgctggc gccggagccg gccgccttgt
     6601 tccgtctgct gaaccgtccg ccggcctggg tgagcgccgg tgacacccag gatctccagc
     6661 agtcgacgga agagttctcc gtggtgctgc gggacatggc cggcctgcag gagcgcatca
     6721 agctgctcca ggaagagatc gtcgcccaca tcggcgagcg caccaaccgc agcgtcttcg
     6781 tgctgaccgc ggtgaccgtg attgccctgc ccatcaacct gacggcgggc ctcttcggca
     6841 tgaacgtggg tggcattccg ctggcggagg atccggaagg gttctggcgg gtgctcctgc
     6901 tggtgctgct gatgaccgcc ggtgcggcct ggtacgcctt ccggcgcggg cccgagtagg
     6961 gcaccggccc agcggccggg cgtggcgtgc cggacgcgcc tccgcgccgg gcggccatcc
     7021 ctgcaagggg gctttcaggc gaggggcttt tcccgcttca ggcggtagct gaactggggt
     7081 cgggtcaggc ccagcagccg cgcggcaccg gacaggttgc cctgctggcg ctgcatggcc
     7141 tcgcggagca gggcggcttc caggtcgtcg agggaaatcc cagacgcgaa cactgcgtcg
     7201 cacagggcgc tgacgtccgg ggcggcgtcc gctgtcgccg ggcgcaggcc gccgctggcg
     7261 tccacgcgat cctcgctgca gggcgaaatg tcgaggcccg cgaagaggtg ttcggcctcg
     7321 atccagccgt cgtgggcggc gaggatcacg ccccgttcca tcatgttctc cagttcccgc
     7381 acgttgcccg gccacttgtg ggccttgagg gcgcgcaggg ccttgtcggt gacgcccttg
     7441 agtcgcttgg cgtggacggc gcagaagcgc tccagcatcg cgtgggccag gggggggatg
     7501 tcagagaccc gctcgcgcaa gggggggatg accaccgggt agacgttgag gcggtaatac
     7561 aggtcgctgc ggaagcggcc gtcccgcacg gccgcctgca ggtccacgtt ggtggcggcc
     7621 accaggcgga tgttgatctt gcgcgggcgg tcgtcgccga ggcgctccac ctcgccgtcc
     7681 tggagcacgc gcaggagctt ggcctgggcg gcgaggggca tctcgccgac ctcgtcgagg
     7741 aacagggtgc cgccgtcggc ccgctcgaac ttgccggcgc gggaagcgtg ggcgccggtg
     7801 aaggcgccct tctcgacgcc gaagagctct gactcgatca ggtcgtgggg cagggcggcg
     7861 cagttgacgg cgacgaaggg cgcgtcccgg cggctgccca gttcgtgcag ggcgcgggcg
     7921 aagcgctcct tgccgacgcc ggtctcgccc agcagcagca cggtcacctg ggtgggggcg
     7981 gccttcttga tgagggtgta ggcgtgctgg aagctcgccg aggcgccgat gaggctgggg
     8041 atctggtggc tggcgccgag ggttgagcgc agggtgtcca cctcgtcgcg caattccagg
     8101 atgcggccga cgacggcggt gtcgtcgtag tagcgcagca gctcggcggc gtcgggccat
     8161 tcttcggccg gcttgccaac gatgcggcag gtgttggcgc ctgcggccgc gcattccacc
     8221 tcgcggaaca ggatgaagcg ccccatgaag caggacgaat agcccgaggc atagccgatc
     8281 tgcatccagc acaccgggtg gtcggcgggg ccgaattccc gtacatgggc atccacttcc
     8341 caggagttct tccacagcag ttcgccgtag aaactgccgg tgtccgggtt catgtcgagg
     8401 cggatcggtt cgacctgcac gctgccttcc agcatgtgga gctgggggcc gacgcagaag
     8461 gcgtcgagca tgtcacggga gccgcgcacc ttgcgggcca gctcggcgtc acgcatgccg
     8521 gcgccgaagc ccatgcgggt cagcacgcga cgggcctggt cctcgccgac cgagtggatg
     8581 agctccttgc gcagggcctg catggtggtc acctggagca gcagcatgcg gctctcgccc
     8641 agccagatca ggccgtcgtc cgccgaaaag cggatgagct cccgcaggtc cttgttctcc
     8701 gggtacttga tggtcaccgt tgtgtctcct ccgtacgccg tgccggcgcg catgggtggt
     8761 cccatgtctg atgtcgcccg tccggggatc ggggcggggg tggcggggcc tgtgccgcac
     8821 cagagggggt ggtgggcaga ttgtcgctgc cgctatcgta caaaatctcg agattttatg
     8881 caatccaccg gacgatatgg atccggcgtg atcgcggttc cacgacgccg tgtgaggttt
     8941 caaggccgtg gggcctgccc gccatcctgc cggagattgt cctccgatag gcgtgacgca
     9001 gtgtgcatct gtgtgacgtg cagcccgtgc gtggcgcttc cgtgccctgc agcggacgtc
     9061 cgagcgcttg gccgcgcggc catgttgcgg tgcgcaagtc tttgttgcaa cgcgaaaaag
     9121 tggcttcacg atttcagcag ccagggttga accatttaat caattgatca atgcaatcgc
     9181 agtgtttatc cgcaaatctc gagaatatta tgtttaatcc gagataaatc agcagcttgc
     9241 cgacactggc acagggcttg attacatggg gccggactcg acaaaagtga atcaagggga
     9301 cgaccgacat gaaatcagac acgccggcag tcgatgtgaa ccggaagttc gtgcgcctgg
     9361 tggaacgacg cgcgaacggg ctggtggagt tcgagttctc catcggcgat ccggagctgt
     9421 tcgtggaaat gctgctgccc gccaccgcct tcgaggagtt ctgcgcggtg aatcgggtgg
     9481 aaatccttga agggtcccgt ccgcacgagg ccggttccga ctgggactgg tccctgcacg
     9541 aggccacgca ccagcgcttc cgctgaggtc cgcagccgcg gacatccaac acaagacaca
     9601 ggaggagaca ccatgcagat tgacttgagg accgtggcga tcaacccgct gcgccagacc
     9661 tatgaccaca tcgcccgacg catcgggccg gacaaggcgg cgtcgcggta ccaggaaggc
     9721 accctggacc tgcaggccac ccacaacttc cattaccgcc cgacctggga ccccgatcac
     9781 gagatcttcg acgcctcgcg ctcggcgatc cgcatggccg actggtacgc cttcaaggat
     9841 ccgcgccagt tctactacgg cgcttacacc ctggcccgcg ccaagcagca ggacaccgcc
     9901 gaggcgaatt tcgactttgt cgagaaccgg ggccttgccg cgaccctgcc ggaagacctg
     9961 cgcgccaccg tgctggcggt gctgctgccg ctgcgccatg tggcctgggg cggcaacatg
    10021 aacggcgcct tcatcgccgc ctacggctat ggcacggcgc tgacccagcc cagcctgtac
    10081 cacgcgatgg atcagctcgg catcgcccag tacctcaccc gcgtcggtct gctgctgggc
    10141 gacgacccct ccgtgctgga cgacgccaag acgcgctgga tggaggacgg cgtgtggcag
    10201 ggcctgcgcc gctacgtgga agacaccttc gtgctgaagg atccgttcga gctcttcgtg
    10261 gcccagatgg tggccctgga cggactgctc tacccgctgg tgtacgaaac catcgtcgat
    10321 gacgtcctct ccgccaacgg cggcactgcc gtggcgatgc tcacccagtt catgaccgaa
    10381 tggttcgccg agacccgcaa gtgggtggac gccaccgtca aggccgcggc cgccgaatct
    10441 cccgagaaca aggcgctgct ggaaaagtgg atcggccact ggcgcgaccg tgcggccgct
    10501 gccctgatgc cagtggccca gatcgccctg gggtcccagg ccgacgcggt gaccagcgag
    10561 ctcgtgtccc agttcaacac ccgcctggcc aaggccggcg tcagcctgtg agaggaagcg
    10621 gcatgtccaa ggtattcatc gccctgcaaa ccaatgacga gacccgccag atcatcgacg
    10681 cgatcctcgc cgacaacccc cacgccatcg cggtcaatca gcccgccatg gtgaagatcg
    10741 atgcggaggg ctccctcgtg gtacgccgct ccaccatcga ggaactcctc ggtcgcgact
    10801 acgacctgca ggaactgcac atcaacctga tctccctcac cggtcacgtg gacgagaccg
    10861 acgaggagct gaccctcagc tggaacgcct gagacacacg gagacacgca catggacatg
    10921 aaagtcagca agaagaagct gggcctcaag gaaaagtacc agcacatgac ccgcggcctg
    10981 ggctgggaga ccagctacca gcagatggac gacgtgttcc cgtacgccaa gtacgagggc
    11041 atcaagatcc atgactggga caagtgggaa gacccgttcc gcctcaccat ggacgcctac
    11101 tggaagtacc aggccgagaa ggaacgcaag ctctacgcga tcatcgacgc ctacgcccag
    11161 aacaatggcc acctgtcggt gaccgatgcc cgctacatca acgccatcaa gctcttcctc
    11221 aacggcatca gcccgctgga atacatggcg caccggggct tcgcccacgt gggccggcag
    11281 ttccccggcg tcggaccgcg ggtcgcctgc ctgatgcagt ccctggacga gatccgccac
    11341 gcccagacgc agatccacgc gctgtccaat tacaacaagt actacaacgg cttccacgaa
    11401 ttccgccaca tgatcgagcg ggtctggtac ctgtcggtgc cgcgctcctt cttcgacgat
    11461 gccgtcaccg ccggtccctt cgagttcatg gtggccatcg gcttcagctt cgagtacgtg
    11521 ctcaccaatc tgctcttcgt gcccttcgtg tcgggtgcgg cctacaacgg cgacatgggc
    11581 accatgacct tcggtttctc ggcccagtcc gacgaggccc gccacatgac cctgggcctg
    11641 gaggtcatca agttcatcct cgaacaggat cccgacaacc tgcccatcgt gcagcgctgg
    11701 atcgacaagt ggacctggcg cggctaccgc gtgctgaccc tggtgggcat gatgatggac
    11761 tacatgctgc ccaagcgcat catgagctgg aaggaagcct gggaggtgta tttcgaggag
    11821 aacggcggcg ccctgttcaa ggatctcgcc cgttacggca tccgtccgcc caagtggctt
    11881 gaccagatcg cctttgacaa ggaccacctg tcccaccagg cctgggccac cttctaccag
    11941 tacggcgccg tgtgcaactt ccacacatgg atgcccaccg acgacgagat ggactggctg
    12001 tcgaagaagt acccgaacag cttcgacaaa tattaccggc cgcgcctgga gttctggcgc
    12061 gacgaggccg ccaagggcaa ccgcttctac aacaagacgc tgcccatgct gtgccagacc
    12121 tgccagatcc ccatgctctt caccgagccg ggcgatccca cccagatctg ctatcgccag
    12181 gtggactaca aggacaacac ctaccacttc tgctcggacg gctgcaaaga gatcttcgag
    12241 cacgagcccg agaagtacat ccaggcctgg ctgccggcac agcagatcta ccagggccac
    12301 tgcttcaacg aaggggtgga tcccacggcc gagggcttcg acccgctggg cgcggtgctc
    12361 gactactacc acatggccgc aggccgcgac aacttcgact tcgacggttc ggaagaccag
    12421 aagaattttg aagcctggcg cgggctggcc accaagaacg cgtgacgggc gcggggcggg
    12481 cgcacgtccg tcgttccgct ccggcccatc cacaccagac acgaggagac atcaccatgg
    12541 gcgttgcagc catcaaggac tacaccttcg agcccgccga ccggctcgag aacttccacg
    12601 gcaaccagtt gctgaacatc ggctgggacc agcacctgct gttctgtgcg ccgttctgcc
    12661 tgcccctgcc gccgacgacg cgctttggcg acatcgtcga caagatcctg ccgggcatct
    12721 tcggctatca cccggacttc gcgaagatcg actggagcac cgtgcagtgg ctcaagggcg
    12781 gtcagccctg gcagccggat ttcgccagga cccttgcgga actggggctc aggcacaagg
    12841 acgcgctgcg cttccgcacg ccggggctca acggcatcca gggctcggcg agctgagcat
    12901 gggctacgaa ctgaccatcg agcccctcgg ccagaccatc gaggtggagg aggggcagac
    12961 catcctcgat gcgtccctgc gcgccgggat ctggctgccc cacgcctgct gtcacggcct
    13021 gtgcgccacc tgcaaggtgc aggtggtgga cggcgaagtg gatcacggcg aagcctccag
    13081 cttcgccctg atggacttcg agcgcaccga aggcaagacg ctggcctgct gcgcccgcct
    13141 ggaaagcgac gtgaccatcg aggcggagat cgacgaggag ccggatgcgg aatccatccc
    13201 ggtgaaggac ttcagcggca cggtgagccg catcgaaacc ctcacgccca ccatcaaggg
    13261 gctgttcttt actctgaacg agccgctcca tttccaggcc gggcagtacg tcaatctgga
    13321 gatcccgacc ctgggcatca gccgggcgtt ctccctggcc aacgtgccct ccacggggcg
    13381 cgaggtggag ctcaacgtgc gcatcgtgcc cggcggcgcc ggcacgggct atctgcacga
    13441 gcgcctgaag gtgggtgacc cgatccgcct gtccggcccc tacgggcgct tcttcgtgcg
    13501 caagtccgcg caggtgccgc tgatcttcat ggcggggggc tcgggtctgt cgagtccgcg
    13561 cagcatgatc ctggacctgc tggctgaagg cagcaccctg ccgatcaccc tggtgtacgg
    13621 ccagcgccgc cgtgaggagc tgtattacca cgacgagttc gtcgagctcg ccgcgcgctt
    13681 tcccaacttc agctacgtac cggccctgtc ggacgagccg gcggattcgg ggtggacggg
    13741 ccatcgcggc ttcgtccatg aggccgccaa ggcccatttc gacaacgact tccggggtca
    13801 caaggcctac ctgtgcggtc cgccggccat gatcgacgcg tgcatctcca cgctgatgca
    13861 ggggcgcctg ttcgagaacg acatctatac ggagaagttc ttcagcgccg ccgatgccca
    13921 gcaggtgcgc agcccgctgt tcaggcgtgt gtaatctgcg ccttaatttg tcgatagaaa
    13981 aataaaatct cgataaaaaa ttgacttgtc gagaagctgc attaaactag caaccagttc
    14041 cgccgagccc acaggaggcc accaagcccc gtcggcggaa gactctcaaa aggaagagga
    14101 gacaatcatg gcaatgactg gcgtgctgcg ccccggccac atgcaactgc gcgtgctgga
    14161 cctggaagaa ggcgtgaatc actacaagaa cgtg
//
LOCUS       DAOQMM010000098        14037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1797_length_14037_cov_44.792662, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000098 DAOQMM010000000
VERSION     DAOQMM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14037
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1797_length_14037_cov_44.792662"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..765)
                     /locus_tag="PLH85_13875"
     CDS             complement(4..765)
                     /locus_tag="PLH85_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB65701.1"
                     /translation="MASISPSKSGRVAAALIAGAAVAAGLAWTIREHRPALPDDPLQL
                     QILADAARRDDALARLERLARDGNVPARRALGLTLIHRALRPAEGLDWLRQAARAGDT
                     DAAFQLGKLLQGGAPGVPVDPAEAVQWLVPAAQADHPGAAYYLGIARRNGYGIAPDPA
                     EAARWFAASARHNVPAALFMLANAYRAGEGVPRNDAEAVRLYEAAAEQDHPEALQTLA
                     MAWRNGEMGLPRDEAQAQHYEMETAHALKHPALKP"
     gene            complement(743..2791)
                     /locus_tag="PLH85_13880"
     CDS             complement(743..2791)
                     /locus_tag="PLH85_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HNB65702.1"
                     /translation="MTFRRSELTAACLIACLLLAGCGGSDSPASTATATPVAPKGTTA
                     TLALLETTDLHTNVMSYDYFKLAEDQSLGFERTATLINAARKEFPNTLLLDNGDTIQG
                     TALSDYQALVSPIACDQTLAIYKVMNAAGFEGGGIGNHEFNYGLPYLSQVTGNRFNVT
                     GMADPSAQPVCKGPSFPQVLANVFSVKTEQPLFQPYTIINKVVTGTGPDGKSVSGTVK
                     VGIIGFTPPTILSWDKRWLDGKVYTLGVKEAAEKYIPEMRAKGADLVVVISHGGLDNS
                     TYSPTMENGSYHLSKVAGVDAMLIGHSHQIFPNATSTVAQFNLPGVDKTAGTVNGVPT
                     VMANYWGKHLGVIKFELAFDGSTWTLDKTKTVVQARSTQNADKTYVAVDATVGAAIAA
                     EHAATIAYVKTPIGSTDYEMNSYFADVGDPGAIELVNQAQADYVAKYVAANLPQYASL
                     PVLSVSAPFKSGFAGGTDYTDVAAGNMAINNAADLYLYPNTVYAVKVTGADIKNWLET
                     AAKRFNQINPALTTQQELVSSYPGYNFDMFTTADIQYEIDVTQAVGSRIKNLSYKGTA
                     IDPAQSFIVATNNYRASGGGSFPGLDGSKTIYASPDANRDVLIAYIKAAKTITRAANG
                     AHKSWRFTKVSTAGPVVLHSAQGKLALAQAAGLGGVSLLQADDGSGKGLALYGIDLSK
                     "
     gene            3026..3898
                     /locus_tag="PLH85_13885"
     CDS             3026..3898
                     /locus_tag="PLH85_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNB65703.1"
                     /translation="MGLRLKFNLVLLAVFMAGFGVAGYISHGLLDRHAREQVLGEARL
                     VMEAALAVRGYTVAQVRPHLSAQMDKQFLPQTVPAFAATETLNELRKKYPNYAYKEAT
                     LNPTNPRDRAADWEADLVNTFRNTADSKEIAGERDTPTGRALYIAHPIRIESPACLAC
                     HSTPEAAPASMIKVYGTANGFGWKLNETVGAQVVSVPMSVPLENAAQAFRTFMTSLAA
                     VFAAVFVVLNLMLSWLIIRPVSQMSDAADKISTGDFDQPEFPEQGTDEVAVLASSFNR
                     MRRSLEKAMQMIDG"
     gene            3906..5960
                     /locus_tag="PLH85_13890"
     CDS             3906..5960
                     /locus_tag="PLH85_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB65704.1"
                     /translation="MSDTPDASAAPPPRDDALILPPGYEINEYRIERVLGGGGFGLTY
                     LARDTHLDCRVAIKEYLPRELAGRVAGRAVRPHSETHAPRFERGLQNFLQESRALASF
                     HHRGIVRVLRYFRANDTAYMVMEYETGQPLHKWLVGRLPLDKATLVRIIRPLLEGLEA
                     IHDAGFLHRDIKPGNIYIRADGSPVLLDFGSARRLQSDGADQTLTTVVTPGFAPAEQY
                     HRHGKQGPWTDLYALAGVMYWMVTGRQPIEAFARLKEDIMPSASSLASADIYGGALLR
                     AIDWALDPDENRRPRKVADFRRVCLAPPTGGEGSDAPVEAPLPFGLGSSEARTFIGAV
                     LFADVGEARPGEEDEHKARRSKLATLLGQTVAPQPATTRLAINTREGCAVCYVGDPED
                     ALRTAVQLGDAAAGQGLPLHMGINLGPVRVEPDPKGRSRILGDGITTAFRVMRFAEPG
                     QVLVARTYFDVIQHLKRNAGACFRHLGLRRDPQARDHDVFLYTGEPLPGTVHVAEASR
                     PTQAMGADITPEVVDAAERLLAQHIGPMARILVRKTVPRAASRDELHRLLAALIPDPA
                     PQAAFLSGVLLASGAPSLSAHPATGPVTRGTAAVSAGSAPASSASVAAPISSVIDEAD
                     LDRVGKLFARHIGPMAKVLLRREARQANSLETLCRALASHIDKDAERQRFLTDAGFGG
                     LG"
     gene            6006..8723
                     /locus_tag="PLH85_13895"
     CDS             6006..8723
                     /locus_tag="PLH85_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB65705.1"
                     /translation="MSEHESPPAAPSRRNWYWTVPYIAVLVFALAMLVLVWILQRQAA
                     DTQRNALARDVQWAEQTMRLHMQGTEEFLAQLAREKAAGTLDGDGFQVRANQHIANNA
                     ELVNVVWLDADERVRWTAPFDTTDWLEGDALSPVQAQTFHRIRDQGRPAYGDPYVNAR
                     GMAVLEFYVPVLQGRTVVGAIVAVYSIERMVSHLVPSWFAEKYRLAFTGPRGESMAMN
                     SAAHPLDENLSFSVPVDPPGNGLTLNATAYSTESALPRGLPMAVIGVLSFFVLWSLWL
                     LRAHMLRRVRVEKERDRIFNLSLDMLAIIGLDGTFRRCNPAFRAILGYAPEDLPGRPL
                     LDFLHAEDVAATVRELRNLASGVPGTFENRCRCADGRYKWLVWSVNSVPEEKLMYAVA
                     HDITGRKSAEEALRAEYAFRKAMEESLVTGLRAIDLSGRIIYVNPAFCRMTGWSREEL
                     VGAVAPFPYWPREEYATLRGNLELTLAGNAPPTGFEMRIQRKNGERLDARFYLSPLID
                     GDGRQTGWMASITDITEPKRVRAELEAAHERLVAVMDGLDAAVYVADAADDEILFANR
                     AFKSIYGFDAVGRPVGAFGLPTPPDTQWYDVDPRRLAPRDVPRELYDGELRHGLSGRW
                     YHIRERAIRWVDGRVVRMAIATDVTDRKRLDEENRVQQERLQRTSRLITMGEMASTLA
                     HELNQPLAAIANYAMGCVNRLQSGEYRQEDILAAMQKASAQAERAGRIIRRVREFVKK
                     SEPRRSPVALAGIVEDAIGFVEVDARRHAAQVVSEIPADLPPVYADAVMIEQVVLNLL
                     KNGIEAMRDTPPEARIVTLGAVALPDGLVEVSVADRGHGLGDDALDKLFSPFYTTKSE
                     GMGMGLNICRSIVEFHQGRLWVDPNPAGGCIFRFTLPLETARESVRDAA"
     gene            8713..9342
                     /locus_tag="PLH85_13900"
     CDS             8713..9342
                     /locus_tag="PLH85_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB65706.1"
                     /translation="MPPEPAAEHIVYLVDDDEALRDSLVWLLESQGFQVAAYASAEDF
                     LANWQPDLSGCLLLDVRMPGMNGLELHERLKARFSTLPVIFITGHGDVPMAVSALKGG
                     AADFIEKPFNDADLLRLVSQCLSREREEGARRRQGAEVSRRLAQLTVREREVLDLIIA
                     GKLNKQIADTLGISIKTVEVHRARVMEKMGAQSLAELVQAVLGVEAGVR"
     gene            9459..10328
                     /gene="folD"
                     /locus_tag="PLH85_13905"
     CDS             9459..10328
                     /gene="folD"
                     /locus_tag="PLH85_13905"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNB65707.1"
                     /translation="MTARILDGNALSAKLRGELAARAATLTAQGVQPCLAVILVGGDP
                     ASAVYVRNKVAGCEKAGIKSLKYEYPQDAAPADVLGRIAALNADPAVHGILVQLPLPP
                     QFDEKAVLEAISVEKDVDGFHAENVGRLSQGQDAFVPCTPNGVMEMLKSENVPLRGAE
                     AVVIGRSNIVGKPMAMLLTAAGATVTVCHSATRDLAFHTRRADILVAAVGRPRFVTGD
                     MIKPGAVVIDVGINRLTDGPDAGKLCGDVDFASAAPVAGLITPVPGGVGPMTITMLLA
                     NTVQSAGRVARKG"
     gene            10332..10829
                     /gene="purE"
                     /locus_tag="PLH85_13910"
     CDS             10332..10829
                     /gene="purE"
                     /locus_tag="PLH85_13910"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNB65708.1"
                     /translation="MNAANPVVGIVMGSNSDWPTMQAAAKVLRDFGVPFEARVVSAHR
                     TPDLMFEYAEQARARGLQAIIAGAGGAAHLPGMLAAKTTVPVLGVPVQSKALSGKDSL
                     LSIVQMPKGIPVATFAIGEAGAANAGLFAVALLAGGDAALAARLEAFRAEQTASVLAM
                     TLDPI"
     gene            10846..12000
                     /locus_tag="PLH85_13915"
     CDS             10846..12000
                     /locus_tag="PLH85_13915"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNB65709.1"
                     /translation="MILPPATLGMLGGGQLGRFFVAAAHEMGYKVWVLDPDPHSPAGL
                     IADRHLQAGYDDFAALDALAAGCAAVTTEFENVPADTLDYLAKFVPVRPGAAAVAVCQ
                     NRIVEKTFLQDNGLPHGPFVPVRSEADLAAAPAELFPAVLKVARFGYDGKGQARVANV
                     AEAVHAFHAFKGEACVLEALLTLDCEVSVVLARGEDGTVSAFPVVENAHSRGILDVSI
                     APARVAPALADEARRCASLIAERLGFIGTLAVEFFVSGGHLVVNEMAPRPHNSGHHTI
                     DACVVSQYEQQVRALCGLPLGEARQHSASVMVNLLGELWYEGLDPHGRYREPDWSVLH
                     AVPGLRLHLYGKHHARHGRKMGHFTVIGDDPAQVLDAALAARRAIGIRDI"
     gene            12013..13026
                     /locus_tag="PLH85_13920"
     CDS             12013..13026
                     /locus_tag="PLH85_13920"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB65710.1"
                     /translation="MHDQAADLDRAVRLLAAGDLVGIPTETVYGLAADARNPAAVARI
                     FAAKGRPADHPLIVHLADAAQLDAWARDIPDAARRLAAACWPGPLTLILKRQPDVPDA
                     VTGGQDTVGLRIPAHPLTLALLRAFAAAGGSGAVAAPSANRFGRISPTIAEHVHQELG
                     DRVAQVLDGGPCAVGLESTILDLSRDVPVILRPGAIGADDIARVIGLRPWQRGEAPAA
                     PDPAVSPAPRVSGALAAHYAPRTPLELVPADRLTERAEAGDAVLARRPSPAGLRPAVT
                     WIEAPADAAAYGHDLYARLRALDASGCARILVEAPPDAPAWAAVADRLGRAAVGSGAP
                     EAT"
     gene            complement(13246..13554)
                     /locus_tag="PLH85_13925"
     CDS             complement(13246..13554)
                     /locus_tag="PLH85_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65711.1"
                     /translation="MKINLIPLGARFEYEGEIYTKTGPITAASEKSGQRMIPRYAVLR
                     PVGELPPAPPPAAERQIAVDTVCAAFEVYHAVALRHVDDFARPALEAARQHFLAALTR
                     "
     gene            13913..13988
                     /locus_tag="PLH85_13930"
     tRNA            13913..13988
                     /locus_tag="PLH85_13930"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13946..13948,aa:Ala,seq:cgc)
BASE COUNT         2004 a         4874 c         4959 g         2200 t
ORIGIN      
        1 ccctcacggc ttcaaggccg gatgcttcag cgcgtgggcg gtctccatct cgtagtgctg
       61 ggcctgggct tcgtcgcggg gcaggcccat ttcgccgttg cgccaggcca tggcgagggt
      121 ctggagggct tccgggtggt cctgttcggc ggcggcttcg tagaggcgga cggcttcggc
      181 gtcgttgcgg ggcacgcctt cgccggcgcg ataggcgttg gcgagcatga agagggcggc
      241 gggcacgttg tggcgggcgg atgcggcgaa ccagcgggcg gcttcggccg ggtcgggggc
      301 gatgccgtag ccgttgcggc gggcgatgcc caggtagtag gcggcgccgg ggtggtcggc
      361 ctgggcggcg gggacgagcc actgcaccgc ttcggcggga tcgaccggaa cgccaggggc
      421 gccgccctgg agcagcttgc cgagctggaa ggcggcgtcg gtgtcgcccg cacgggcggc
      481 ctggcgcagc cagtcgaggc cttcggcggg gcgcagtgcg cggtggatga gggtgaggcc
      541 gagggcgcgg cgggcgggca cgttgccgtc gcgggcgagg cgctccaggc gggcgagggc
      601 gtcgtcgcgg cgggcagcgt cggcgaggat ctggagctgg agcgggtcgt cgggcagggc
      661 cgggcggtgt tcgcggatcg tccaggcgag gccggcggcc accgccgccc cggcgatcag
      721 ggcggcggcg acgcggccgg acttacttgg agagatcgat gccatacagc gcgaggccct
      781 tgccgctgcc gtcgtcagcc tggagcaggc tcacgccgcc gaggccggcg gcctgggcga
      841 gggcgagctt gccctgggcg gagtggagca ccacggggcc ggccgtgctc accttggtga
      901 agcgccagct cttgtgggcg ccgttggcgg cgcgggtgat ggtcttggcg gccttgatgt
      961 aggcgatcag cacgtcccgg ttggcgtcgg gcgaggcgta gatggttttg ctgccgtcca
     1021 ggccggggaa gctgccgccg ccgctggcgc ggtagttgtt ggtggcgacg atgaaggact
     1081 gggccgggtc gatggccgtg cccttgtagc tgaggttctt gatgcggctg ccgacggcct
     1141 gggtgacgtc gatctcgtac tggatgtcgg ccgtggtgaa catgtcgaag ttgtagccgg
     1201 ggtaggaact gacgagttcc tgctgggtgg tgagggcggg gttgatctgg ttgaagcgct
     1261 tggcggcggt ttcgagccag ttcttgatgt cggcgccggt gaccttcacc gcatagacgg
     1321 tgttggggta gaggtagagg tcggcggcgt tgttgatggc catgttgccg gccgccacgt
     1381 cggtgtagtc ggtgccgccg gcgaagccgc tcttgaacgg ggcgctgacg gagagcacgg
     1441 gcaggctggc gtactggggc aggttggcgg ccacgtactt ggccacgtag tcggcctggg
     1501 cctggttgac cagctcgatg gcgcccggat cgcccacgtc ggcgaagtag ctgttcatct
     1561 cgtaatccgt gctgccgatg ggggtcttga cgtaggcgat ggtggcggcg tgctcggcgg
     1621 cgatggcggc gcccacggtg gcatcgacgg ccacgtaggt cttgtcggcg ttctgggtgg
     1681 agcgggcctg gacgacggtc ttggtcttgt cgagggtcca ggtgctgccg tcaaaggcga
     1741 gttcgaactt gatcacgccc aggtgcttgc cccagtagtt ggccatcacg gtgggcacgc
     1801 cgttcacggt gccggcggtc ttgtccacgc cgggcaggtt gaactgggcg acggtgctgg
     1861 tggcgttggg gaagatctgg tgggagtggc cgatcagcat ggcgtccacg ccggcgacct
     1921 tggagaggtg gtagctgccg ttctccatgg tgggcgagta ggtactgttg tcgaggccgc
     1981 cgtgggagat caccaccacc aggtcggcgc ccttggcgcg catctcgggg atgtactttt
     2041 cggcggcttc cttgacgccc agggtgtaga ccttgccgtc gagccagcgc ttgtcccagc
     2101 tcaggatggt gggcggggtg aagccgatga tgccgacctt gacggtgccg ctgacgctct
     2161 tgccgtccgg gccggtgccg gtgacgacct tgttgatgat cgtgtagggc tggaacaggg
     2221 gctgctcggt cttcacgctg aagacgttgg cgagcacctg cgggaagctc gggcccttgc
     2281 acacgggctg ggcggacggg tcggccatgc cggtcacgtt gaagcggttg ccggtgacct
     2341 ggctgaggta gggcaggccg tagttgaatt cgtggttgcc gatgccgccg ccttcaaagc
     2401 cagcggcgtt catgaccttg tagatggcca gggtctggtc gcaggcgatg gggctcacga
     2461 gggcctggta gtccgacagg gcggtgccct ggatcgtgtc gccgttgtcc agcagcaggg
     2521 tgttggggaa ttccttgcgg gcggcgttga tgagcgtggc ggtgcgctcg aagccgaggg
     2581 actggtcctc ggcgagcttg aagtagtcgt aactcatcac gttggtgtgg aggtcggtgg
     2641 tctccagcag cgccagcgtg gcggtggtgc ccttgggggc gacgggcgtc gccgtggcgg
     2701 tggacgcggg ggaatcgctg ccgccgcagc cggccagcag caggcaggcg atcaggcagg
     2761 cagcggtgag ttcggaacga cggaaggtca tggcacgcac ctgggagggg aaaggttcct
     2821 cccagcttcg cgcggcgaca ttgcctgtca gtgaacggca ccttataaat tgaagacggc
     2881 agggtgaagt tgcggtgacg gcgcccgccc atggtggatc gccctgcggc tgcccgcgaa
     2941 tgcgaatggg gcgcaatacg cggcccgcct gtgccagaat gtcacccggt ttgccggacg
     3001 accggcgatt gaggagggta tagcgatggg tctccggctt aaattcaacc tggtcttgct
     3061 ggccgtcttc atggcgggct ttggcgtggc gggttacatc tcccatggcc tgctcgaccg
     3121 ccatgcccgc gagcaggtgc tcggcgaagc gcgcctggtg atggaggccg ccctggcggt
     3181 gcggggctac acggtggccc aggtgcgccc gcacctgtcg gcgcagatgg acaagcagtt
     3241 cctgccccag accgtgccgg cctttgcggc caccgaaacc ctcaacgaac tgcgcaaaaa
     3301 gtatcccaac tacgcctaca aggaagccac cctcaacccc accaaccccc gcgaccgggc
     3361 ggcggactgg gaggcggacc tggtgaatac cttccgtaat acggccgaca gcaaggagat
     3421 tgccggcgag cgcgacaccc ccaccggccg cgccctctac atcgcccacc cgatccgcat
     3481 cgagtcgccg gcctgtctgg cctgtcacag cacgccagag gcggcgcccg cctccatgat
     3541 caaggtgtat ggcaccgcca acggctttgg ctggaagctc aacgaaacgg tgggcgccca
     3601 ggtggtgtcg gtgcccatgt cggtgccgct ggagaacgcg gcccaggcct tccgcacctt
     3661 catgacatcc ctggcggccg tgttcgcggc agtcttcgtg gtgctcaacc tgatgctgtc
     3721 gtggctcatc atccggcccg tgtcgcagat gtccgacgcc gccgacaaga tcagcaccgg
     3781 cgacttcgat cagccggagt tccccgagca gggcaccgac gaagtggccg tgctggccag
     3841 ttccttcaac cgcatgcgcc gcagtctcga gaaggccatg cagatgatcg acggttgagc
     3901 gcttcgtgag cgatacaccg gacgcgtccg ccgccccacc gccccgggac gacgcgctga
     3961 tcctgccgcc cggctacgag atcaacgaat accggatcga acgggtgctg ggcggcggcg
     4021 gcttcgggct cacctacctg gcccgcgaca cccacctgga ctgccgggtg gcgatcaagg
     4081 agtatctgcc gcgggaactc gccgggcggg tggccgggcg cgcggtgcgg ccccattccg
     4141 agacccacgc cccgcgtttc gagcggggac tgcagaactt cctccaggaa tcccgcgccc
     4201 tggccagctt ccatcaccgg ggcatcgtgc gggtgctgcg ctacttccgc gccaacgaca
     4261 cggcctacat ggtcatggag tacgagaccg gccagcccct ccacaagtgg ctggtgggcc
     4321 gcctgcccct cgacaaggcg accctggtgc gcatcatccg cccgctgctg gaaggcctcg
     4381 aggcgatcca cgacgcaggc ttcctgcacc gggacatcaa acccggcaac atctatatcc
     4441 gcgccgacgg ctcgccggtg ctcctggatt tcggctcggc ccggcggctg cagagcgacg
     4501 gcgcggacca gacgctcacc accgtcgtca cgccgggctt tgcgccggcc gagcaatacc
     4561 atcgtcacgg caagcagggg ccgtggaccg acctgtacgc cctggcgggg gtgatgtact
     4621 ggatggtcac cgggcgccag ccgatcgaag cctttgcgcg cctgaaggaa gacatcatgc
     4681 cgtcggcgag ctcgctggcc agcgccgaca tctacggcgg cgcgctgctg cgggccatcg
     4741 actgggcgct ggatcccgac gagaaccggc ggccccgcaa ggtggccgac ttccgccgcg
     4801 tctgtctggc accgcccacc ggtggcgaag gctcggacgc cccggtggag gcgccgctgc
     4861 ccttcggcct ggggtcgtcc gaggcgcgta ccttcatcgg cgcggtgctg tttgccgacg
     4921 tgggcgaggc gcggccgggc gaggaagacg aacacaaggc ccgccgctcg aagctggcca
     4981 ccctgctggg ccagaccgtg gcgccccagc ccgccaccac gcggctggcc atcaacaccc
     5041 gcgaaggctg cgcggtgtgc tacgtgggcg acccggaaga cgccctgcgc accgccgtgc
     5101 aactgggcga cgccgccgcc ggccagggcc tgcccctgca catgggcatc aacctgggcc
     5161 cggtgcgggt cgaacccgac cccaaggggc gctcgcgcat tctcggcgac ggcatcacca
     5221 cggccttccg cgtcatgcgc tttgccgagc ccggccaggt gctggtggca cgcacctatt
     5281 tcgacgtgat ccagcacctc aagcgcaacg ctggcgcatg tttccgtcac ctgggcctgc
     5341 gccgcgaccc ccaggcgcgg gatcacgacg tcttcctcta caccggcgag cccttgcccg
     5401 ggaccgtgca tgtggccgag gcgtcgcgcc cgacgcaggc gatgggcgcc gacatcacgc
     5461 cggaggtcgt cgatgcggcc gagcgcctgc tcgcccagca catcggcccg atggcgcgca
     5521 tcctggtgcg caagaccgtg ccccgcgctg ccagccggga cgaactgcac cgcctgctcg
     5581 ccgcgttgat tcccgatccg gcgccccagg ccgccttcct gtccggcgtg ctgctggctt
     5641 cgggcgcgcc gtcgctctcc gcccacccgg ccaccgggcc ggtcacgcgg gggacggcgg
     5701 cggtgtccgc cgggtcggcg ccggcctcct cggcaagcgt cgcggcgccg atctccagcg
     5761 tcatcgacga ggcggacctg gaccgggtcg gcaagctctt cgcccgccac atcgggccca
     5821 tggccaaggt gctgctgcgc cgcgaggcgc gtcaggccaa cagcctcgaa accctgtgcc
     5881 gtgccctcgc cagtcacatc gacaaggacg cggagcggca gcgcttcctg accgacgccg
     5941 gattcggggg attagggtaa gacccgatgt cgcccgggcc gtatcgcaag cacgatgacg
     6001 gttccatgtc cgaacacgaa tcacctcccg ctgccccgtc ccgccgcaac tggtactgga
     6061 cggtgcccta catcgccgtg ctggtgttcg cgctggccat gctggtgctg gtgtggatcc
     6121 tccagcgcca ggccgccgac acccagcgca acgccctcgc ccgcgacgtg cagtgggccg
     6181 agcagaccat gcgcctgcac atgcagggca ccgaggagtt cctcgcccag ctggcccgcg
     6241 agaaggccgc cggtacgctg gacggggacg gcttccaggt gcgcgccaac cagcacatcg
     6301 ccaacaacgc cgagctggtg aacgtggtgt ggctggacgc cgacgagcgg gtgcgctgga
     6361 ccgccccctt cgacaccacc gactggctgg aaggcgacgc cctgtcgccg gtgcaggccc
     6421 agaccttcca ccgcatccgc gaccagggcc gccccgccta cggcgacccc tacgtcaacg
     6481 cccgcggcat ggcggtgctg gagttctacg tgccggtgct gcagggccgt accgtggtgg
     6541 gcgccatcgt cgccgtgtat tccatcgagc gcatggtgag ccacctggtg ccgagctggt
     6601 tcgccgagaa ataccggctc gccttcaccg ggccgcgggg cgagtccatg gccatgaact
     6661 ccgccgccca tcccctcgac gagaacctgt ccttctcggt gccggtggac ccgccaggca
     6721 acgggctgac cctcaacgcc accgcctatt ccaccgaaag cgcgctgccc cgcggcctgc
     6781 ccatggcggt gatcggcgtg ctgtccttct tcgtgctgtg gagcctgtgg ctcttgcgcg
     6841 cccacatgct gcggcgggtg cgggtggaga aggagcgcga ccggatcttc aacctgtccc
     6901 tcgacatgct cgccatcatc ggtctggacg gcaccttccg gcgctgcaat ccggccttcc
     6961 gcgccatcct cggctacgcg ccggaggacc tgccgggccg gccgttgctg gatttcctgc
     7021 acgccgaaga cgtggccgcc acggtgcgcg agctgcgcaa cctggcttcc ggtgtgcccg
     7081 gcaccttcga gaaccgctgc cgctgcgccg acgggcgcta caagtggctg gtgtggagcg
     7141 tgaactcggt gcccgaggaa aagctcatgt acgcggtggc ccacgacatc accgggcgca
     7201 agtctgccga agaggccctg cgcgcggaat atgccttccg taaggcgatg gaggaatccc
     7261 tcgtcaccgg cctgcgcgcc atcgacctga gcggccgcat catctacgtg aacccggcct
     7321 tctgccgcat gaccggctgg tcgcgggagg aactggtggg ggccgtcgcg ccgttccctt
     7381 actggccgcg ggaggaatac gccaccctgc ggggcaacct ggagctcacc ctggccggca
     7441 acgcgccgcc cacgggcttc gagatgcgca tccagcgcaa gaacggcgag cgcctcgacg
     7501 cgcgcttcta cctgtcgccc ctcatcgacg gcgacggccg ccagaccggc tggatggcct
     7561 ccatcaccga catcaccgag cccaagcggg tgcgtgccga gctggaggcc gcccacgagc
     7621 gcttggtggc ggtgatggac ggcctcgatg cggcggtgta cgtggccgac gctgccgacg
     7681 acgagatcct cttcgccaac cgcgccttca agagcatcta cggcttcgac gcggtaggcc
     7741 ggccggtggg cgccttcggc ctgcccaccc cgcccgacac ccagtggtac gacgtggacc
     7801 cgcgccgcct ggcgccccga gacgtgcccc gcgagctcta cgacggcgag ctgcgccacg
     7861 gcctgtccgg gcgctggtac cacatccgcg agcgcgccat ccgctgggtg gacggccgcg
     7921 tggtgcgcat ggccatcgcc accgacgtta ccgaccgcaa gcgcctcgac gaggagaacc
     7981 gcgtccagca ggaacgcctg caacgcacct cgcggctcat caccatgggc gagatggcgt
     8041 cgaccctggc ccatgaactc aaccagcccc tggcggccat tgccaactac gcgatgggct
     8101 gcgtcaatcg cctgcaatcc ggcgaatatc gccaggaaga catcctcgcc gccatgcaga
     8161 aggccagcgc ccaggccgag cgggccggcc gcatcatccg ccgggtgcgc gaattcgtga
     8221 agaagagcga accgcggcgc agcccggtgg ccctggcggg catcgtggag gacgccatcg
     8281 gcttcgtcga ggtggacgcc cgccgccacg ccgcccaggt ggtcagcgag atcccggccg
     8341 acctgccgcc ggtttacgct gacgcggtga tgatcgagca ggtggtcctc aatctcctca
     8401 aaaacggcat cgaggccatg cgcgacacgc cacccgaggc gcggatcgtg accctcggcg
     8461 ccgtcgcgct gcccgacggg ctggtggagg tctccgtcgc ggaccggggc cacggcctcg
     8521 gcgacgatgc cctcgacaag ctgttctcgc ccttctacac caccaagagc gagggcatgg
     8581 gcatgggcct caacatctgc cgctccatcg tcgaattcca ccagggccgc ctgtgggtgg
     8641 accccaaccc ggccggtgga tgtatcttcc gtttcaccct gcctctggag actgcccgtg
     8701 aatccgtccg agatgccgcc tgagccggcc gccgaacaca tcgtctacct cgtggacgac
     8761 gacgaggccc tgcgcgactc cctcgtctgg ctgctggagt ctcagggctt ccaggtggcg
     8821 gcctacgcct cggccgagga cttccttgcc aactggcagc ccgacctctc cggctgcctg
     8881 ctgctcgacg tgcgcatgcc cggcatgaac ggcctggagc tccacgagcg cctcaaggcg
     8941 cgcttcagca ccctgccggt gatcttcatc accggccacg gcgacgtgcc gatggcggtg
     9001 tcggccctca agggcggcgc cgcggatttc atcgagaagc ccttcaacga cgccgacctg
     9061 ctgcgcctcg tgagccagtg tctcagccgc gagcgggagg agggtgcccg ccgccgccag
     9121 ggggccgaag tgtcacggcg gctggcccag ctcacggtgc gtgagcggga ggtgctggac
     9181 ctgatcatcg ccggcaagct caacaagcag atcgccgata cgctgggtat cagcatcaag
     9241 acggtggaag tccatcgcgc ccgggtcatg gagaaaatgg gcgcccagtc cctcgccgag
     9301 ctggtgcagg cggtgctggg cgtcgaggcc ggcgtgcgct gagagcgtgc ggcggcccgc
     9361 gaccgccgtc cggtgcgggc cgacaccctg ttgaaaacgc tggggatttc cccttgcgcc
     9421 gtggtacact ccgcggtttt tctttgaggg gcgcaggcat gacggctcgc attctcgatg
     9481 gcaacgcgct ttccgccaaa ctgcggggcg aactcgccgc ccgggcggct acgctcaccg
     9541 cccagggcgt ccagccctgc ctggcggtga tcctggtggg cggcgatccg gcctccgccg
     9601 tgtatgtccg caacaaggtg gccggctgcg aaaaggccgg catcaagtcc ctcaagtacg
     9661 aatacccgca ggatgccgcg ccggccgacg tgctcggccg catcgcagcg ctcaacgccg
     9721 acccggcggt gcatggcatc ctcgtgcagt tgcccctgcc gcctcaattc gacgagaagg
     9781 ccgtccttga ggcgatctcc gtcgagaagg acgtggacgg cttccacgcc gaaaacgtgg
     9841 gccgcctgtc ccagggccag gacgccttcg tgccgtgcac gcccaacggc gtgatggaga
     9901 tgctcaagag cgagaacgtg cccctgcgcg gtgccgaggc ggtggtcatc ggccgctcca
     9961 acatcgtcgg caagcccatg gcgatgctcc tcacggccgc cggcgccacc gtcaccgtgt
    10021 gccattcggc cacccgcgac ctggccttcc acacccgccg cgccgacatc ctggtggcgg
    10081 cggtgggcag gccgcgcttc gtcaccggcg acatgatcaa gcccggtgcg gtggtcatcg
    10141 acgtgggcat caaccgcctc actgacggcc cggatgccgg caagctgtgc ggcgacgtgg
    10201 atttcgcctc ggccgccccg gtggccggcc tcatcacccc ggtgcccggc ggcgtcgggc
    10261 ccatgaccat caccatgctg ctcgccaata ccgtccagtc ggccgggcgc gtcgcgcgaa
    10321 agggctgaat catgaacgct gccaatcccg tcgtcggcat cgtcatgggc tccaattccg
    10381 actggcccac catgcaggcc gcagccaagg tgctgcgcga cttcggcgtg cccttcgagg
    10441 cccgcgtcgt ctctgcccac cgcacgcccg acctgatgtt cgaatacgcc gagcaggccc
    10501 gcgcccgcgg tctccaggcc atcatcgccg gcgccggcgg tgccgcccac ctgcccggca
    10561 tgctcgccgc caagaccacg gtgccggtgc tgggcgtgcc ggtgcagtcc aaggccctgt
    10621 cgggcaagga ttccctgctc tccatcgtgc agatgcccaa gggcattccg gtggccacct
    10681 ttgccatcgg cgaggcgggc gccgccaacg ccggcctctt tgcggtggcg ctgctggccg
    10741 gcggcgatgc ggccctggct gcgcggcttg aagccttccg cgccgagcag accgccagcg
    10801 tgctggccat gaccctcgat ccgatctgat tccggggccg acgcgatgat cctcccgccc
    10861 gccaccctcg gcatgctcgg cggcggccag ctcggccgct tcttcgtggc tgcggcccac
    10921 gagatgggct acaaggtctg ggtgctggac ccggaccccc acagcccggc cggcctcatc
    10981 gccgaccgcc acctgcaggc cggctacgac gacttcgcgg ccctcgacgc cctcgccgca
    11041 ggctgtgccg cggtcaccac cgaattcgaa aacgtccccg ccgacaccct ggactacctc
    11101 gccaagttcg tgcccgtgcg gcccggtgcg gcagcggtgg ccgtgtgcca gaaccgcatc
    11161 gtcgagaaga ctttccttca ggacaacggt ctgccccacg gccccttcgt gcccgtgcgc
    11221 agcgaggccg atctggccgc cgcgcccgcc gagctgttcc ccgcggtcct caaggtggcc
    11281 cgcttcggct acgacggcaa gggtcaggcc cgcgtcgcca acgtcgccga ggccgtgcac
    11341 gccttccacg ccttcaaggg cgaggcctgc gtgctggagg ccctgctgac cctcgactgc
    11401 gaggtctcgg tggtcctggc gcgtggtgag gacggcacgg tgagcgcctt tccggtggtg
    11461 gagaacgccc acagccgtgg catcctggat gtctccatcg cgcccgcgcg ggtcgccccg
    11521 gccctggccg acgaggcgcg ccgctgcgcc agcctcatcg ccgaacggct cggcttcatc
    11581 ggcaccctgg cggtggaatt cttcgtcagc ggaggccacc tggtggtcaa cgagatggcg
    11641 ccgcggcccc acaacagcgg ccatcacacg atcgacgcct gcgtcgtcag ccagtacgag
    11701 cagcaggtgc gcgccctgtg cggcctgcct ctgggcgagg cgcgccagca cagcgcgtcg
    11761 gtgatggtca atctgctggg cgagctgtgg tacgagggcc tcgatcccca cggccgttac
    11821 cgggagccgg actggtcggt gctgcacgct gtgccgggcc tcaggctgca cctgtacggc
    11881 aagcaccacg cccgccacgg tcgcaagatg gggcacttca cggtgatcgg cgacgacccc
    11941 gcgcaggtgc tcgacgctgc cctggcggcc cgccgcgcca tcggcatccg cgacatctga
    12001 gcggcaccgt ccatgcacga ccaggctgcc gatctcgacc gggccgtgcg gctgctggcg
    12061 gcgggcgatc tggtgggcat ccccaccgag accgtttacg gcctggccgc cgacgcccgc
    12121 aatcctgccg cagtggcgcg catctttgcc gccaaagggc ggccggcgga tcatcccctc
    12181 atcgtccacc tcgccgacgc ggcccagctc gacgcgtggg cgcgggacat ccccgatgcc
    12241 gcccgccgcc tcgccgccgc gtgctggccg gggcccctga ccctcatcct caagcgccag
    12301 cccgatgtgc cagacgccgt caccggcggg caggacaccg tgggtctgcg catccccgcc
    12361 catccgctga ccctggcgct gctgcgtgcc tttgccgcgg cgggtggcag cggcgccgtg
    12421 gcggcgccct ccgccaaccg cttcgggcgc atcagcccga ccatcgccga gcatgtgcac
    12481 caggaactgg gcgatcgcgt ggcccaggtc ctggacggcg gcccctgcgc ggtggggctg
    12541 gagtccacca tcctcgacct gtctcgcgat gtaccggtga tcctgcggcc cggcgccatt
    12601 ggcgccgacg acatcgcccg ggtgatcggc ctgcgaccgt ggcagcgtgg cgaagcgccc
    12661 gctgcgcccg acccggccgt gtcgcccgca ccccgcgttt cgggtgctct ggccgcccat
    12721 tacgcgccgc gcacgccgct ggaactggtg ccggcagacc gcctcaccga acgggcagag
    12781 gccggtgacg cggtgctggc gcgtcgcccg tcgccggccg gcctgcgccc cgctgtcacc
    12841 tggatcgagg cgccggccga tgccgccgcc tacggtcacg atctctacgc ccgcctgcgc
    12901 gccctggacg cctcgggttg cgcccgcatc ctcgtcgagg cgccccccga cgcgccagcc
    12961 tgggcggccg tggccgaccg cctgggccgc gcggccgtgg gctccggcgc gccggaagcg
    13021 acctagaccc gcccggccgg attccggggg cgcccccacg cgctgcccga cgcgcgcgtg
    13081 gtcgccggga tcccgtcagg gccccggccg atggagcacg ggccctcggc accgggcccg
    13141 ccggacggtc tgggcgatgc actttctgag tacgttggtt gcgggcggtg ggttgatgca
    13201 aatgcccggt ctgggacggc cgggcgaggg atgtcgggga cgccgtcagc gcgtcagcgc
    13261 tgcgaggaag tgttggcgcg ccgcctccag cgcggggcgg gcgaagtcat ccacgtgacg
    13321 cagggcgacc gcgtgataga cctcgaaggc ggcgcagacg gtatccaccg cgatctgccg
    13381 ctccgccgca ggcggtggcg cgggcgggag ttcgcccacg gggcgcagga ccgcgtaacg
    13441 gggaatcatg cgctgtccgc tcttttcgga ggcagcggtg atggggccgg tcttggtgta
    13501 gatctcgcct tcgtattcga agcgggcgcc aagggggatc aggttgattt tcatgttcag
    13561 ggcctgcaag ggggcgggtg gggcgcagcg gggattgtaa tgaacccggc tgtgacttcc
    13621 cagccggtgg tcagtggagg cgcgctgtgc gcgattggcg cgggacggtc gacgcggacc
    13681 ggggcggttg cggtccccat ggccatgagg agcgcgcgtc cgtgtgccga cggtgccgga
    13741 gctcgattgc gaattgctgc gtccgggccg cgtgccttgt cggtcctggg gcacacgccg
    13801 ggccatcgaa gcggcacgca ccgcttcagt gaggatccag ctggcgccgt gccgcgggca
    13861 cgacaaccgc tttgcaagcg ttgagcgctc cgctataatg cgcggctcaa gtgggccgat
    13921 agctcagctg ggagagcgcc gcgttcgcaa tgcggaggtc gtgagttcga tcctcattcg
    13981 gtccaccacg aacacttccg gaatgttccg gagaaagccg ggaaagccaa ggaaatc
//
LOCUS       DAOQMM010000099        13676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1883_length_13676_cov_46.979664, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000099 DAOQMM010000000
VERSION     DAOQMM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13676
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1883_length_13676_cov_46.979664"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1425)
                     /locus_tag="PLH85_13935"
     CDS             complement(190..1425)
                     /locus_tag="PLH85_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNB65712.1"
                     /translation="MKRAPPLPDPVPTDTRVPLRPLDELQVPPAFDGHDGINRADAAR
                     LQIAATTDPEAIACFLSEYDRSPGTYRIYQRECERLLLWAIVEVGKPLSSLNRQDFEG
                     YLNFLADPQPAVLWCGPKVARASEKWRPFVGPLSESAVLTAVAAINSLMSYLVDAGYL
                     AGNPLGLIRQRRRKMAAEAGGPLRAVASTDEVEKIERFLDAQMWDAVTAAIEALPRDS
                     ERERDEYERARFIAATLYLLAPRAGELESHRMNSFREERGLWWWHVVGKGAKKAKVPV
                     PEDMLQALIRYRKYLGLSAVPRRDDSTPLLISVKDRSPITARRLNQILKKIFHAAAEL
                     LPPEVEHKKEKLRAASAHWGRHTGITAKLDAGMDQRYVQKDARHTDARTTQRYIHEEE
                     ERWHDEAQKQKLPWAGKKP"
     gene            1620..1781
                     /locus_tag="PLH85_13940"
     CDS             1620..1781
                     /locus_tag="PLH85_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65713.1"
                     /translation="MENMICRVLMGGGLLAGVLLAGGQGEQALGPVVLGVLTAMVLRR
                     MRPLFPRRA"
     gene            complement(1782..2453)
                     /locus_tag="PLH85_13945"
     CDS             complement(1782..2453)
                     /locus_tag="PLH85_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNB65714.1"
                     /translation="MTSHLLAVCRRAALAGAFATALCALPAGAATLDDVINAAKLGDA
                     AEIAQEVSRGLDPNVTDANGNTLLIIAAREDQPRVVEALLKYRVKINARNPVGDTALM
                     LAALRGNTRVVELLLQAGAAPDHSGWNPLLYASFEGHTAIVDMLLARGASPDATAPNG
                     ATALMFAAKGGHQDVVARLLKAGADTERANDRGDTAESWALQAHNTDIADMIRAARSA
                     RATTR"
     gene            complement(2450..3223)
                     /locus_tag="PLH85_13950"
     CDS             complement(2450..3223)
                     /locus_tag="PLH85_13950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB65715.1"
                     /translation="MFVDSHCHLDFPGLIEHEAEHLARMADNGVSHALCVSVKLETLP
                     AVIGLAERHAHLFASVGVHPDNADCDEPDEARLVALAAHPKVVAIGETGLDYYWHKDA
                     PEWQRQRFRTHIRAARACSKPLIVHTRSAAADTLRLMREEGADAVGGVMHCFTESLEV
                     AREALDLGFYISFSGIVSFKNAEDLRVVARMVPADRLLVETDAPYLAPVPFRGKTNQP
                     AYVRHVAEAVAAARGESLDAVADATTRNFFRLFKDAHAS"
     gene            complement(3294..3662)
                     /locus_tag="PLH85_13955"
     CDS             complement(3294..3662)
                     /locus_tag="PLH85_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNB65716.1"
                     /translation="MNEPTKRATAARPSVLSLNINSKSALYAAYMPFVKNGAIFVPTV
                     RDYKLGDEIFMLLQIMDDSTKHPVAGTVVWVTPPNAQGNKTQGIGVSFAGDEPGQVLR
                     NRIEQILAGHLGSSRPTHTI"
     gene            complement(3751..4815)
                     /gene="holB"
                     /locus_tag="PLH85_13960"
     CDS             complement(3751..4815)
                     /gene="holB"
                     /locus_tag="PLH85_13960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNB65717.1"
                     /translation="MIFEWHKPLWQRVLDNSGRMHHALLLNGPAGGGKRAFAEALAAR
                     TLCVQPDADGQACGRCEDCQWRLAGSHPDFMRIIPEADQNDTESSSEKTGETKAASRQ
                     IVIDQIRDLQQSLTTGGHRGGQRVVIVDPAEAMNPYTANALLKLLEEPTTSTLFLLIT
                     STPKRLLPTIRSRCQSWAFPRPPADAAVRWLDAQQIAEAPALLAFAGGMPLAAAALAT
                     GGGAAALRRFTADIAGLPGKSALRLAGEWDAWLKSKEAQGCGLDMPTLTLWLQRWVGD
                     LASVCLGGPLRFFPDSRGVLQSLAERLAVDRVLGCYNDVTQSRRVAQHPLNARLFLED
                     MLLRYARAFSTGGARTGGGR"
     gene            complement(4812..5429)
                     /gene="tmk"
                     /locus_tag="PLH85_13965"
     CDS             complement(4812..5429)
                     /gene="tmk"
                     /locus_tag="PLH85_13965"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNB65718.1"
                     /translation="MKPRFITFEGIDGAGKSSQIAAAVALLRTRGLNVVQTREPGGTP
                     LGEKLRELLLHDAMHLETEALLMFAARREHLAALIEPALAAGSWVVSDRFTDATYAYQ
                     VGGRGLDEGRFAALERWVHPHLQPDLTFIFDLDPAIAAQRLAGTGVAPDRFEREQTDF
                     FRRVRAAYLRRAAADPGRVRVIDAGRTPDEIQKELEEIIVSHCLK"
     gene            complement(5426..6427)
                     /gene="mltG"
                     /locus_tag="PLH85_13970"
     CDS             complement(5426..6427)
                     /gene="mltG"
                     /locus_tag="PLH85_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNB65719.1"
                     /translation="MKRFIVRCLALGVALVALLAAALAWYAHEPMGLRTSPLDFTIEP
                     GAGMRQVARQVVDGGIEVQPVLLTWLARITGQANSIKAGSYEVERGVTPLQLLAKLSR
                     GDVSQADLTLVEGWNIRQFRAALDRHPDLRHDSAGLSDAELLTRLGIDARHPEGQFFP
                     DTYLFARRSSDLEVLRRAHDNLQRRLAREWDKRADGLPYASPYEALIMASIVEKETGL
                     PADRPKIASVFVNRLRAGMLLQTDPTVIYGLGAAFDGNLRKRDLLADTPYNTYTRAGL
                     PPTPIAMPGLASLRAALNPPVTDYLYFVARGDGSSAFSRTLDEHNRAVAQYIRKRNS"
     gene            6613..7644
                     /locus_tag="PLH85_13975"
     CDS             6613..7644
                     /locus_tag="PLH85_13975"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNB65720.1"
                     /translation="MNAAWFTHLGAQGARFTDPYVRFHDDARQDVRAAEQGTVLVPLT
                     HLAAIRATGEDAGALLHNLFSNDVKKLGPNEAQLTSFNSAKGRMLASLLLWREGADYL
                     LALSADIHAAVLKKLGMYVLRSKAKLLDDSGDSVLIGLAGPQAGAALEAAGLGIPGTP
                     RATASGIATVVRLDNARFIVSAPLSEATQLWERLRASGAVPAGTAAWQWLDIREGLPT
                     VTLPVQEEFVPQMINYELIGGVSFNKGCYPGQEIVARTHYLGKLKKRMYRVHGDTADL
                     PAAGADLYAPEFGEQSAGKVVMAAPAPEIGWDALVVLQTTTAEAGVVHLGAPDGPQLG
                     FLPLPYALT"
     gene            7652..7942
                     /locus_tag="PLH85_13980"
     CDS             7652..7942
                     /locus_tag="PLH85_13980"
                     /inference="COORDINATES: protein motif:HMM:NF027614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HNB65721.1"
                     /translation="MTPSHFYIWYPVRIGLEADLARTLYNLQSDVAERTGVAGRFLRK
                     ADDPWTWMEIYENVRDADAFARVLEEAVERHGLARFVDDGGHRHPERFVSCA"
     gene            7933..8712
                     /locus_tag="PLH85_13985"
     CDS             7933..8712
                     /locus_tag="PLH85_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNB65722.1"
                     /translation="MCLILVAWQARADYPLVVAANRDEFFARPAAAAAWWDDAPALFA
                     GRDLEAGGTWLGLSRQGRFAALTNFRDPARVRAGAPSRGALVADFLQGRESTDDALAR
                     LAAEGPRYNGFNLLLSDGVTLGVFESEADSGRGAGRRLAPGLYALSNHLLDTPWPKVS
                     AGKSRLARALDALPDDTPLRDLLRDDRPAPDAALPRTGVSLAWERMLSSAFIRAPGYG
                     TRCSTVIVRDRFGWTRVVESTWDAHGRESGRVAERFQIQPA"
     gene            8789..12235
                     /gene="mfd"
                     /locus_tag="PLH85_13990"
     CDS             8789..12235
                     /gene="mfd"
                     /locus_tag="PLH85_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNB65723.1"
                     /translation="MTRPTHPFSALLPELDAVADARPGQRLDLPALAGSADALAIARL
                     AGGGRMLVVITAHPVDAQRLAEELAWLAPDLRTHLLPDWETLPYDSFSPHQDLISERL
                     STLYAITRGEADIALVPATTAVSRLAPPAFLAAYTFFLKQGEKLDVNGLREQMTVAGY
                     AHVTQVMSPGEYSVRGGLVDLYPMGAALPYRVDLFDEDIESIRTFDPDTQRTVFPVKE
                     VRLLPAREFPLDEAGRTRFRGRFRETFEGDPSRCTIYKDISNGIAPAGIEYYLPLFFD
                     STATLFDYLPASAALFLHRDVAGAIDAFWKDVRSRHKLLDGDKTRPVMAPEQLFLGSE
                     SFFVALRDRPRLQLGTSAGDGPARPLPDVAVERKASDPLHRFKAFCAGFAGRVLVLAE
                     SPGRQQTIAEYFAEYGLQPTATTDFDAFAGGDARLALATGPLAGGFVLPGAGLAVVTE
                     SELYAATARTRTRRENRKSASVEGWLRDLSELKAGDPVVHSSHGIGRYMGLVHMDLGE
                     GDTEFLHLEYANGDKLYVPVAQLHVITRYAGADPEAVHLHTLGSGQWEKAKKKAAQQV
                     RDTAAELLALYAQRAARKGHQFDFKQHDLEAFADGFGFEETPDQRAAIEAVVTDMKSG
                     RPMDRLVCGDVGFGKTEVALRAAFIAVADGKQVVVLCPTTLLAEQHYQTFSDRFADWP
                     IKVAELSRFKNAKEQADAIQQLGEGKVDILIGTHRLLQKDVVFKRLGLVIIDEEHRFG
                     VRQKEALKALRSEVDILTLTATPIPRTLGLALEGLREFSVIATAPQKRLAIKTFVQPY
                     NKGIVREAVLREFKRGGQVYFLHNEVETIVNVREALEELLPEARIVVGHGQLPERELE
                     RVMRDFTTQKANLLLCTTIIETGINIPTANTIIIHRADRFGLAQLHQLRGRVGRSHHQ
                     AYAYLLTDAHAKPTAQAQKRLEAISMMEELGSGFYLAMQDLEIRGAGEVLGENQSGEI
                     QQVGMSLYTEMLKRAVRDLQQGREPDLSQPLEVVSEINLHAPALLPSDYCSDVQERLT
                     LYKRLANCDSEDDLRALQEELIDRFGELPPQTRTLLETHRLRMLVRPYGVAKLDASDS
                     QITLHFGANAAIDPVKVILLIQKDKSVRMSGPDKLVRKVATPDLSQRVKAVRDLLVAV
                     KA"
     gene            12429..12881
                     /locus_tag="PLH85_13995"
     CDS             12429..12881
                     /locus_tag="PLH85_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNB65724.1"
                     /translation="MSATLLAFPGLSAAGLLGGEQLLHATPRLGIDWIPLVRRGLPAA
                     SLDALVRQTRIARTDLAEALAIPERTLARRKREGQLSPEESAKLVRLARTVERAEEVF
                     EDAAAALAWLRQDNAALGGVTPLSLLDTDLGADAVLDTLGRIEHGVFA"
     gene            12881..13366
                     /locus_tag="PLH85_14000"
     CDS             12881..13366
                     /locus_tag="PLH85_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNB65725.1"
                     /translation="MPVVWRITTARFAASAFSGEGARRYGGRWNPRGTPLIYTAESRA
                     LALLEMLVQDDPLRARYVLIPAHIPDDLPLDRLDADALPADWRTLGARDALQTLGRRW
                     LEGPHRAALIVPSAVVPAEHNVLIDPTHPDFTRIAIGPVEALETDMRLLRNLAAQPRG
                     A"
     gene            13465..>13676
                     /locus_tag="PLH85_14005"
     CDS             13465..>13676
                     /locus_tag="PLH85_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65726.1"
                     /translation="MFPPDPPRRTGPLVFALGISLLVHGLILFLAPRTPLDAGRPAPT
                     LQARLEARQAREPAAASAPPPASAAP"
BASE COUNT         2108 a         4995 c         4470 g         2103 t
ORIGIN      
        1 actaccggga aggccagccc ttcgacgtct ccctcagcga cgtccagttc gaagttcccg
       61 acatcgacgc cccggcgctg gtgcgggacg tggggcgacg gctggtgtaa agcgaacgga
      121 aggtgcgtcg tcgggagcgc gcctgccgcg caacgggcgt ccgctcgcgt agtctgcggc
      181 ctcagtccgt caaggcttct tccccgccca aggcagtttc tgcttctgtg cctcgtcgtg
      241 ccagcgctct tcctcttcgt ggatgtagcg ctgcgtggtg cgggcgtccg tgtggcgggc
      301 gtccttctgc acgtagcgct ggtccatgcc ggcgtccagc ttggcggtga tgcccgtgtg
      361 gcggccccag tgggcggagg cggcgcggag cttttccttc ttgtgctcca cctcgggcgg
      421 cagcagctcg gcggcggcgt ggaagatctt cttgaggatc tggttgaggc ggcgggcggt
      481 gatggggctg cggtccttca ccgagatgag cagcggcgtg ctgtcgtcgc ggcggggcac
      541 ggcgctgagg cccaggtatt tgcggtagcg gatgagggcc tgaagcatgt cctcgggcac
      601 cggcaccttg gccttcttgg cgcccttgcc caccacgtgc caccaccata ggccgcgctc
      661 ctcgcggaag ctgttcatgc ggtggctttc cagctcgcct gcgcggggcg cgaggaggta
      721 gagggtggcg gcaatgaagc gggcgcgctc gtattcgtcc cgctcccgct cggaatcccg
      781 cggcagggcc tcgatggcgg cggtgacggc gtcccacatc tgggcgtcga ggaagcgctc
      841 gatcttctcc acctcgtcgg tgctggccac ggcccgcagc ggtccgcccg cctcggcggc
      901 catcttgcgg cggcgctggc ggatgagccc gaggggattg ccggccagat agcccgcatc
      961 caccaggtag ctcatcaggg aattgatcgc cgccacggcg gtgagcacgg cgctctcgct
     1021 gagggggccg acgaagggcc gccacttttc cgacgcccgc gccaccttgg gcccgcacca
     1081 caggaccgcc ggctggggat cggcgaggaa gttcaggtag ccctcgaaat cctgccggtt
     1141 gagactggac aggggcttgc ccacctcgac gatggcccac aggagcaggc gctcgcattc
     1201 ccgctggtag atgcggtagg tgccgggcga gcggtcgtac tcactgagga agcaggcgat
     1261 ggcttcgggg tccgtggtcg cggcgatctg caggcgcgcg gcgtcggcgc ggttgatccc
     1321 gtcgtggccg tcgaaggccg gcggcacctg cagttcgtcc agcggacgca ggggcacgcg
     1381 ggtgtcggtc ggaacggggt cgggcagtgg gggcgcgcgc ttcatgggcg tagggcgatc
     1441 gggcgggggc gcgtcgcagg atacgccgat gtaccgatcg gcgcacgcac ccttgcatcc
     1501 cacacgtagc tgtatataat tacacttatc gaacggatga atccggcctt ccctctggat
     1561 cgccgaaggc gccccggatg cgcagggccg ctgtccgttc ccctactcag gagaagatga
     1621 tggaaaacat gatctgtcgt gtgctgatgg gcggcgggct gctggccggt gtgttgctgg
     1681 ccggtggcca gggcgaacag gccctggggc cggtggtgct gggcgtgctg accgccatgg
     1741 tgctgcgccg catgcggccc ctgttcccgc gtcgggcctg atcaacgggt ggtggcgcgg
     1801 gcgctgcggg cagcgcggat catgtcggca atgtcggtgt tgtgggcctg cagggcccag
     1861 ctttcggcgg tgtcgccccg gtcgttggcg cgctcggtgt cggctccggc cttgagcagg
     1921 cgcgccacca cgtcctggtg accgcccttg gcggcaaaca tcagcgccgt ggcgccgttg
     1981 ggcgccgtgg catccgggct tgcgccgcgg gcgaggagca tgtcgacaat agcggtatgc
     2041 ccttcaaaac tggcgtacag aagcggattc cagccggaat gatccggtgc cgcgccagcc
     2101 tggaggagga gttccacgac gcgcgtattt ccgcgcagcg cggccagcat cagggccgtg
     2161 tcaccgaccg ggttgcgggc gttgatcttc acccggtact tgagcagcgc ctccaccacc
     2221 ctgggctgat cttcccgggc cgcgatgatg agcagggtgt tgccgttcgc gtcggtgacg
     2281 ttggggtcca ggccacggct cacctcctgg gcgatctcgg cggcgtcgcc cagcttggcg
     2341 gcattgatca cgtcgtcgag ggtggccgcg cccgcaggta acgcgcacag tgcggtggca
     2401 aaggcgccgg ccagtgcagc acggcggcag acggcgagga gatgcgatgt catgaggcgt
     2461 gggcgtcctt gaagagccgg aagaagttcc gggtcgttgc gtcggccacg gcgtcaaggc
     2521 tttcgccccg ggccgccgcg acggcttccg ccacatggcg gacgtaagcg ggctggttgg
     2581 tcttgccacg gaagggcacc ggcgccaggt agggcgcgtc ggtttccacc agcagacggt
     2641 cggccggtac catgcgggcg acgacccgca gatcttctgc gttcttgaag ctgacgatgc
     2701 cggaaaagga gatgtagaag cccagatcca gggcctcccg ggccacctcc aggctttcgg
     2761 tgaagcagtg catcacacca ccaacggcat ctgcgccctc ctcgcgcatc aggcgcaggg
     2821 tgtcggccgc ggcactgcgg gtatgcacga tgagcggctt ggagcacgcg cgggcggcac
     2881 gaatgtgggt gcgaaagcgc tgccgttgcc attccggcgc gtccttgtgc cagtaatagt
     2941 ccagcccggt ctcgccgatg gccaccacct tggggtgggc cgccagggcc acgaggcggg
     3001 cttcatccgg ttcgtcgcag tctgcgttgt ccggatgcac ccccacggac gcaaacaggt
     3061 gggcgtgacg ctccgccagc ccgatcaccg ccggcagggt ttcgagcttg acgctgacac
     3121 acaaggcgtg gctcacaccg ttgtcggcca tgcgcgcgag gtgctcggcc tcgtgttcga
     3181 tgagtccggg gaaatccagg tgacagtggg aatcgacaaa catgaagagt gtgcgctcac
     3241 aacgaagggg gcgtgcggca cgggcaccag tggatcgtgt tcaggcgcgc cgctcagatg
     3301 gtatgcgtgg gacgggagga gcccagatgg ccggcgagga tctgctcgat gcggttacgc
     3361 aatacctgcc cgggttcatc accagcgaaa ctgaccccga tgccctgggt cttgttcccc
     3421 tgggcgttgg gcggcgtgac ccacacgacc gtgccggcca ccgggtgctt ggtggaatcg
     3481 tccatgatct gcaggagcat gaagatctcg tcgccgagct tgtagtcccg caccgtgggc
     3541 acgaagatcg cgccgttctt gacgaagggc atgtaagccg cgtagagcgc ggacttggaa
     3601 ttgatgttga gcgacagcac gctcggacgg gctgcggtcg cgcgcttcgt gggttcgttc
     3661 atgcgtactc caccggccgg cggtcattgc aaggggccaa acgatgcacg gcgcgcagcc
     3721 gcctggctgc cgctccggag gcccgggcgc tcaccgcccg ccaccggtcc gcgcaccgcc
     3781 cgtgctgaag gcccgggcgt accgcaacag catgtcttcg aggaaaagcc gcgcgttcag
     3841 gggatgctgg gccacccggc gcgactgggt cacatcattg tagcagccca gcacccgatc
     3901 cacggcgagg cgttccgcca gactctgcag cacaccccgg ctgtccggaa agaaacgcag
     3961 tggcccgccc aggcacacgc tggccaggtc ccccacccag cgctgcagcc acagggtcag
     4021 ggtcggcatg tccagcccgc agccctgagc ctccttgctt ttgagccagg catcccattc
     4081 ccccgccaga cgcagggccg acttgcccgg caggccggcg atatctgctg tgaaacgccg
     4141 caatgccgcc gctccgccgc ctgtcgcgag ggctgccgca gccagcggca tgccccctgc
     4201 aaaggccagc agcgcgggcg cctccgcgat ctgctgcgcg tccagccagc gcacggcagc
     4261 gtcggccggc ggacgcggga aggcccagct ctgacagcgg ctgcggatcg tcggcaggag
     4321 tcgtttcggc gtcgatgtaa tcaataaaaa cagagtactt gtagtgggtt cttcaagtaa
     4381 cttgagcagt gcattggcgg tgtagggatt catcgcctcg gccggatcga cgatgacgac
     4441 ccgctgtccg cctcgatggc cgccggtggt gagactttgc tggaggtcgc ggatctggtc
     4501 gatgacgatc tgccgactgg ccgcctttgt ttctccagtt ttttctgaag aagattccgt
     4561 atcattttga tctgcttcag gaatgatgcg cataaaatca ggatgactcc ctgccagacg
     4621 ccactgacag tcttcgcacc gcccgcaggc ctgaccgtct gcatccggct gcacgcacag
     4681 cgtccgcgcc gccagcgctt cggcgaaggc acgtttgccg ccgcccgccg gaccattcag
     4741 cagcaaggca tggtgcatcc gtccggagtt gtccaaaacg cgctgccaga gaggtttatg
     4801 ccactcaaaa atcatttcag gcaatgactt acgatgattt cttcgagttc cttctggatt
     4861 tcgtccggcg tacgtccggc gtcgatcacc cggacccgcc ctggatcggc cgcggcccgg
     4921 cgcaggtagg cagcgcgtac ccgccggaag aaatccgtct gttcccgctc gaaacgatcc
     4981 ggcgccacgc cggtacccgc gagccgttgc gcggcaatgg cggggtccag atcgaagatg
     5041 aaggtcaggt ccggctgcag gtggggatgc acccagcgct ccagcgcagc gaaccgccct
     5101 tcgtccaggc cgcgcccccc cacctggtag gcgtaggtgg cgtccgtgaa gcggtcggag
     5161 accacccagc tgcccgccgc gagggccggc tcgatgaggg ccgcgaggtg ctcccggcgc
     5221 gccgcgaaca tcagcagggc ctcggtttcc aggtgcatgg cgtcatgcag caggagctcc
     5281 cgcagttttt cgccaagggg cgtgccgccc ggctcgcgag tctgcacgac gttcaggccg
     5341 cgggtgcgga gcaaggcgac cgccgccgcg atctggctgc tcttgccggc gccgtcgatg
     5401 ccttcgaagg tgatgaagcg gggtttcacg agttacgttt ccggatgtac tgggcgacgg
     5461 cccgattgtg ttcgtccagc gtgcgcgaga acgcgctgga accgtcaccg cgggccacga
     5521 agtaaagata gtcggtcacc ggcggattga gggccgcccg cagggacgcc agcccgggca
     5581 tggcgatggg cgtgggcggc aggccggcgc gggtgtaggt gttgtaaggg gtgtcggcga
     5641 gcaggtcgcg cttgcgcagg ttgccgtcga aggccgcgcc caggccgtag atgaccgtcg
     5701 gatcggtctg cagcagcatg ccggcgcgca ggcggttgac gaagaccgac gcgatcttgg
     5761 gccggtcggc gggcaggccg gtctcctttt ctacgatgga ggccatgatc agggcttcgt
     5821 agggcgacgc gtagggcagg ccatcggcac gcttgtccca ttcccgggcg aggcgccgct
     5881 gcaggttgtc gtgggcccgg cgcagcacct ccagatcgct ggagcggcgc gcgaagagat
     5941 aggtatccgg gaagaactgc ccttccgggt gacgcgcgtc gatgcccagg cgggtcagca
     6001 gttccgcgtc gctgagcccg gcgctgtcgt ggcgcagatc cgggtgacgg tccagcgcgg
     6061 cgcggaactg gcggatgttc cagccttcca cgagggtcag atccgcttgg ctcacgtcgc
     6121 cgcggctgag cttggcgagc agttgcaggg gcgtcacgcc gcgttccact tcgtagctgc
     6181 cggccttgat ggagttggcc tgaccggtga tccgcgccag ccaggtgagc agcaccggtt
     6241 gcacctcgat gccgccgtcc accacctgcc gagccacctg acgcatgccg gcgccgggct
     6301 cgatggtgaa atccagcggg ctggtccgca ggcccatggg ctcgtgggca taccacgcga
     6361 gggcagcggc gagcagcgcg acgagcgcga cgcccaaggc aagacagcgg acgatgaaac
     6421 gtttcatacg ggaacttggc gcgtccggaa ggaacgaaag aggagggacg agacaatgac
     6481 tgcggactgc acggccggtg tgacgaaggt ggcaccgatc gtgcataccg gccggaaggc
     6541 gcccgtataa tagcccaaag ctcacacatc ctcgctcccg acccttcgag ttcgctcgcc
     6601 cacaggacca ccatgaacgc agcctggttt acccatctcg gcgcccaggg cgcccgcttc
     6661 accgacccgt acgtccgctt ccacgacgac gcgcggcagg acgtccgcgc cgccgaacag
     6721 ggcaccgtac tggtgcccct gacccatctc gccgccatcc gcgccaccgg cgaggatgcc
     6781 ggcgccctgc tccacaacct gttctccaat gatgtgaaga agctcggccc caacgaggcc
     6841 cagctcacca gcttcaacag cgccaagggc cgcatgctcg ccagcctgct gctgtggcgc
     6901 gagggtgccg actacctgct ggccctttcc gccgacatcc acgccgccgt gctcaagaag
     6961 ctcggcatgt atgtgctgcg ctccaaggcc aagctgctgg acgacagcgg cgacagcgtg
     7021 ctgatcggcc tcgccggtcc gcaggcgggc gccgcgctgg aagccgccgg cctgggcatc
     7081 cccggcacgc cgcgggccac cgcgtcgggc atcgccaccg tggtgcgtct ggacaacgcc
     7141 cgcttcatcg tctccgcgcc cctctccgaa gccacccagc tgtgggaacg cctgcgcgcc
     7201 agcggtgccg tgcccgccgg caccgccgcc tggcagtggc tggacatccg cgagggcctg
     7261 cccaccgtga cgctgcccgt gcaggaggaa ttcgtgcccc agatgatcaa ctacgagctg
     7321 atcggcggcg tgagcttcaa caagggctgc tatcccggtc aggaaatcgt cgcccgcacc
     7381 cactacctgg gcaagctcaa gaagcgcatg taccgcgtgc atggcgacac ggccgatctg
     7441 cccgccgccg gggccgacct ctacgcaccg gaattcggcg agcaatccgc gggcaaggtc
     7501 gtcatggccg ccccggcacc ggagatcggc tgggacgccc tggtggtgct gcagaccacc
     7561 accgccgagg ccggcgtcgt ccatctgggc gcgcccgacg gtccgcagct cggtttcctg
     7621 cccctgccct acgctctcac ctgacggtcc cgtgaccccg tcccatttct acatctggta
     7681 tccggtgcgg atcggcctcg aggccgatct ggcccggacc ctctacaacc tgcagagcga
     7741 cgtggccgag cgcaccggcg tcgccgggcg cttcctgcgc aaggccgacg acccgtggac
     7801 ctggatggag atctacgaga acgtgcgcga cgccgacgcc ttcgcccgag tgctcgaaga
     7861 agccgtcgag cgccatggcc tggcccgctt cgtggacgac ggcgggcacc gccatccgga
     7921 acgtttcgtg tcgtgtgcct gatcctcgtc gcctggcagg cccgcgccga ctacccgctg
     7981 gtggtggcgg ccaaccggga tgagttcttc gcgcgcccgg ccgccgcagc cgcctggtgg
     8041 gacgatgcgc cggccctgtt tgccgggcgc gacctggagg cgggcggcac ctggctcggc
     8101 ctgagccgcc agggccgctt cgccgccctc accaatttcc gcgatcccgc ccgcgtgcgc
     8161 gccggcgccc cctcccgcgg cgcgctggtg gcggatttcc tgcagggtcg cgaatccacc
     8221 gacgatgccc tcgcccgcct cgcggcggaa ggcccgcgct acaacggctt caacctgctg
     8281 ctgtcggacg gtgtcacgtt gggcgtcttc gagagcgagg ccgacagcgg ccgcggcgcc
     8341 gggcggcgcc tcgcaccggg cctctacgcg ctctccaacc acctgctgga cacgccctgg
     8401 cccaaggtca gcgccggcaa gtcgcgcctc gcccgcgccc tcgacgccct acccgacgac
     8461 acgcccctgc gagacctgct gcgggacgac cgccccgcgc cggacgccgc cctgccgcgc
     8521 accggcgtca gccttgcctg ggagcgcatg ctgtccagcg ccttcatccg cgctccgggc
     8581 tacggcacgc gttgctccac ggtgatcgtc cgcgaccgtt tcggctggac ccgggtcgtc
     8641 gagtccacct gggacgccca cggccgggaa agcggccggg tggccgagcg cttccagatc
     8701 cagcccgcct gaatcccgca cgcccggcgc ggacgcctat aatccgccct tccccggaac
     8761 cccctccgcg ccgcaccgcg aaccccgcat gacccgcccc acgcatcctt tcagcgccct
     8821 cctgcccgaa ctcgacgccg tggccgacgc ccgtcccggc cagcgcctgg atctgcccgc
     8881 cctggccgga tccgccgacg ccctggccat cgcccgtctg gcgggcggcg ggcgcatgct
     8941 ggtggtgatc actgcccacc ccgtggacgc ccaacgtctg gcggaagagc tcgcctggct
     9001 cgcgcctgat ctgcgcaccc acctgctgcc ggactgggag accctgccct acgacagctt
     9061 ctcgcctcac caggacctga tctccgagcg cctgtccacc ctctacgcca tcacccgcgg
     9121 cgaagccgac atcgccctcg tgccggccac caccgccgtc tcgcgcctgg ccccgccggc
     9181 cttcctggcg gcctacacct tcttcctgaa acagggcgaa aagctcgacg tgaacggcct
     9241 gcgcgagcag atgaccgtcg ccggctacgc ccacgtcacc caggtcatgt cgcccggcga
     9301 atacagcgtg cgcggcgggc tggtggatct ctatcccatg ggcgccgcgc tgccctaccg
     9361 ggtcgacctc ttcgacgaag acatcgagag catccgcacc ttcgacccgg acacccagcg
     9421 caccgtcttc ccggtcaagg aggtgcgcct cctgccggcg cgggagtttc ccctcgacga
     9481 agccggccgc acccgcttcc gcggccgttt ccgcgaaacc ttcgaaggcg atccgtcccg
     9541 ctgcaccatt tacaaggaca tctccaacgg catcgcgcca gccggcatcg agtactacct
     9601 gcccctcttc ttcgactcca cggcgaccct cttcgactac ctgcccgcca gcgctgccct
     9661 gttcctgcac cgggacgtgg cgggcgccat cgacgccttc tggaaggacg tacgcagccg
     9721 ccacaagctc ctggacgggg acaagacccg tccggtaatg gcgccggagc agctcttcct
     9781 gggcagcgag agcttcttcg tcgccctgcg cgaccgcccc cgcctgcaac tgggcacgtc
     9841 cgccggcgac ggtccggcac ggcccctgcc cgacgtggcg gtggagcgca aggccagcga
     9901 cccgctgcac cgcttcaaag ccttctgcgc cggctttgca ggccgggtgc tggtgctggc
     9961 cgaatcgccg gggcgccagc agaccatcgc cgaatacttc gccgaatacg ggctgcagcc
    10021 caccgccaca accgacttcg acgcctttgc cggcggcgat gcgcgcctgg ccctcgccac
    10081 cgggcccctg gccggcggat tcgtgctgcc cggcgccggc ctcgcggtgg tcaccgaatc
    10141 cgagctctac gccgccaccg cgcgcacccg cacgcgccgc gagaaccgca agtccgccag
    10201 cgtggaaggc tggctgcgcg acctgtcgga gctcaaggcc ggcgatccgg tggtccattc
    10261 cagccacggc atcggccgct acatgggtct ggtgcacatg gacctgggcg aaggcgacac
    10321 ggaattcctg cacctggaat acgccaacgg cgacaagctc tacgtccccg tcgcccagct
    10381 ccacgtcatc acccgctacg ccggcgccga cccggaggcg gtgcacctgc acaccctcgg
    10441 ctccggtcag tgggagaagg ccaagaagaa ggccgcccag caggtacgcg acacggccgc
    10501 cgagctcctc gccctctacg cccagcgcgc cgcccgcaag ggccatcagt tcgacttcaa
    10561 gcaacacgac ctggaagcct tcgccgacgg cttcggcttc gaggaaaccc ccgaccagcg
    10621 cgcggccatc gaggccgtgg tgaccgacat gaagtccggc cgccccatgg accgcctggt
    10681 gtgcggcgac gtgggcttcg gcaagaccga ggtcgccctg cgcgccgcct tcatcgcggt
    10741 ggccgacggc aagcaggtgg tcgtcctgtg ccccaccacc ctgctcgccg agcagcacta
    10801 ccagaccttc tccgaccgct ttgccgactg gcccatcaag gtggccgagc tgtcccgctt
    10861 caagaacgcc aaggaacagg ccgacgccat ccagcagctg ggcgaaggca aggtggacat
    10921 cctcatcggc acccaccgcc tgctccagaa ggacgtggtc ttcaagcgcc tgggcctggt
    10981 catcatcgac gaggagcacc gcttcggcgt gcgccagaag gaagccctca aggccctgcg
    11041 cagcgaggtg gacatcctca ccctcaccgc cacccccatc ccgcgcaccc tgggcctggc
    11101 cctggaaggt ctgcgggaat tctcggtgat cgccactgcg ccccagaaac gcctggccat
    11161 caagaccttc gtccagccct acaacaaggg catcgtgcgc gaagccgtgc tgcgggaatt
    11221 caagcgcggc ggccaggtgt atttcctcca caacgaggtg gagaccatcg tcaacgtccg
    11281 cgaagccctg gaggaactgc tccccgaggc gcgcatcgtg gtcggtcacg gccagctgcc
    11341 ggagcgggag ctggagcgcg tcatgcgcga cttcaccacc cagaaggcca acctgctgct
    11401 gtgcaccacc atcatcgaga ccggcatcaa catccccacc gccaacacca tcatcatcca
    11461 ccgggccgac cgcttcggac tggcccagct ccaccagctg cgcggccgcg tcgggcgctc
    11521 ccaccaccag gcctacgcgt acctcctcac cgacgcccat gccaagccca ccgcccaggc
    11581 ccagaagcgc ctggaggcca tctccatgat ggaagagctg ggctccggct tctacctggc
    11641 catgcaggat ctggagatcc gtggcgccgg cgaggtgctc ggcgaaaacc agtccggcga
    11701 gatccagcag gtgggcatga gcctctacac cgagatgctc aagcgcgccg tgcgcgacct
    11761 gcaacagggc cgcgaaccgg acctctccca gcccctggag gtggtctccg agatcaacct
    11821 ccacgccccc gccctgctgc ccagcgacta ctgcagcgac gtgcaggaac gcctcaccct
    11881 ctacaagcgc ctcgccaact gcgacagcga ggacgacctg cgcgccctgc aggaagagct
    11941 catcgaccgc ttcggcgaac tgccgcccca gacccgcacc ctgctcgaga cccaccgcct
    12001 gcgcatgctg gtgcgcccct acggcgtggc caagctggac gcctccgaca gccagatcac
    12061 cctccacttc ggcgccaacg ccgccatcga cccggtcaag gtcatcctgc tgatccagaa
    12121 ggacaagagc gtgcgcatgt ccggcccgga caagctcgtg cgcaaggtcg ccacgccgga
    12181 cctgagccag cgggtcaagg cggtgcggga cctgctcgtc gcggtcaagg cctgagccca
    12241 ccccgcttcg ggtccccgcg ttcagggcgg cggcgggccc gtccgcagtt catcgcggct
    12301 ggcgcagcgc gcggcccgat cgcgcatcgc cctctctagc gctttctgcc aaaattgcct
    12361 atattgaaag ccaaatggca gaccacctca cctgccgcgg cctgcctgcc ctcaccggag
    12421 ccccgaccat gagcgccacc ctcctcgcct ttcccggcct ctccgccgcc ggcctgctcg
    12481 gcggcgagca actcctccac gccacgccgc gcctgggcat cgactggatt cccctggtcc
    12541 ggcgcggcct gcccgccgcg tccctggacg ccctcgtgcg ccagacccgc atcgcccgca
    12601 ccgacctggc cgaagccctt gccatcccgg aacgcactct ggcccggcgc aagcgcgaag
    12661 gccagctgtc gcccgaagaa tcggccaagc tcgtgcgcct ggcccgcacc gtcgaacggg
    12721 ccgaggaagt cttcgaggac gccgccgccg ccctcgcctg gctgcgccag gacaacgcgg
    12781 ccctgggggg cgtcacgccc ctgtccctgc tcgacaccga cctgggcgca gacgccgtcc
    12841 tcgacaccct cggacgcatc gagcacggcg tgttcgcctg atgcccgtcg tctggcgcat
    12901 caccaccgcc cgcttcgccg catcggcctt ctccggcgaa ggcgcccggc gctacggcgg
    12961 acgctggaac ccccgcggca cacccctcat ctacaccgcc gagtcccgcg ccctcgccct
    13021 gctcgaaatg ctcgtccagg acgaccccct gcgtgcccgc tacgtactga tcccggcgca
    13081 catccccgac gatctgcccc tcgaccgcct cgacgccgac gccctgcccg ccgactggcg
    13141 caccctcggc gcccgcgacg ccctccagac cctcggccgg cgctggctcg aaggccccca
    13201 ccgggccgcc ctgatcgtcc ccagcgccgt ggtacccgcg gagcacaacg tgctcatcga
    13261 ccccacacac ccggatttca cccgcatcgc catcggcccc gtcgaggccc tcgaaacgga
    13321 catgcgcctg ctccgcaacc tggctgcgca gccgcgcggc gcctgagcac accgccacgg
    13381 gcgcgcccgc accctgtgcc gccggcctcc cggcatccgc cccacgacag gccgccgtaa
    13441 cccaccgctg ctagaatgcc gccgatgttc ccgcccgacc ctccgcgccg caccgggccc
    13501 ctggtcttcg ccctgggcat ctccctgctc gtccatggcc tgatcctctt cctcgcaccg
    13561 agaacacccc tggacgccgg ccgccccgcg cccaccctgc aagcccgcct ggaggcgcgc
    13621 caggcgcggg aaccggccgc cgccagcgcc ccgccgcccg cctccgctgc cccaca
//
LOCUS       DAOQMM010000100        13508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1913_length_13508_cov_59.105924, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000100 DAOQMM010000000
VERSION     DAOQMM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13508
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1913_length_13508_cov_59.105924"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..1092
                     /locus_tag="PLH85_14010"
     CDS             406..1092
                     /locus_tag="PLH85_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65727.1"
                     /translation="MKNRYVWIGTCSVLAHLVLFWPEPNRPGDGRRPVISVRLPDYAR
                     GQPPKDETLLMESAETVERAVPMENVAHATPEVPNASGRARTIPAHSVSRGSLVDVSP
                     KAAIATSLQTLGRDGTVAEGGDSDDRPGLLRYRIALASAAVKRVRAELSVVVMQDAVH
                     SLTVEVSGLEAGVTAVRIIASSGRAGLDDMVLRAVELAAGDVPHPFVERGKPWAFTLP
                     VSISQELEAL"
     gene            complement(1139..1687)
                     /locus_tag="PLH85_14015"
     CDS             complement(1139..1687)
                     /locus_tag="PLH85_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB65728.1"
                     /translation="MNFCSQCGSPVTVRVPPGDNLPRHVCDACGTIHYQNPKVVVGAL
                     PVWNEQVLLCRRAIEPRYGLWTLPAGFMENNETAAEGAVRETQEEAGARVELGPMFSF
                     VDIPGISQIHIIFQATLLAPDFSPGEETLEAALFDESDVPWNEIAFRSVEFTLRKFFE
                     DRRSGNYRLHTTALEIHPPLRT"
     gene            1849..3843
                     /locus_tag="PLH85_14020"
     CDS             1849..3843
                     /locus_tag="PLH85_14020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB65729.1"
                     /translation="MTSSFDITKFEYLKASGDLPSPKGVALAVMRALQHDDCSTGELA
                     RIIKTDPAFVGRIVKAANGVAGFNRRAVASVQDAILVLGMRAVRTLALGFSLLSEYRN
                     GVSREFDYPRYWSGCLAQALAMQAIAHHTRAGALEDMFCLGLLARVGELALATLYPAE
                     YDTLQRRRRAEPATAIARLEHELFAISHGELGGAMLADWGLPKVFCVAAEFAEHPERA
                     GHEPGSRSDVLTRSLQMAACMAALFVAPETERAQYLPRLIELGGTLDLPPALVGEISD
                     GVAREWQAWGPLLDVETPDIPSFEVLAAQAADMRAREIEMASAPDATETIVLHDAQPG
                     ASRPLGVLVVHDDAETRSLIRRVLEVAGHDVREAREGRRALECVLESPPQLLIVDRDL
                     ADGDGLEFVRNLRQTKIGRGLYVLMLMRAGDDDRLIEAFENGADDFVHTPVSPKILAA
                     RLRAAQRVINLQEELERDREEVRRFAAELAVSNRRLQEMALTDSLTGFPNRRYAMERV
                     AQEWSTASRTRRPLSAMVVDVDEFKRFNDNFGHDTGDTILRQVANSLKTALRAQDVVA
                     RTGGDEFLVICPDTHLDAALACAERVRFAVEAGGHASAGGRVTVSIGVATREAGMNDA
                     EALVKRADQALYQAKNKGRNRITTTQTARPAPLVPPRGTH"
     gene            3996..4313
                     /gene="flhD"
                     /locus_tag="PLH85_14025"
     CDS             3996..4313
                     /gene="flhD"
                     /locus_tag="PLH85_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HNB65730.1"
                     /translation="MRNHALQDEIRELNLAYLMLAQQMLREDRAVALYRLGIGEDVAD
                     VLEGFSAAQLLRMASSTMLLCQFRFNDSMLVDLLASHGRERGTAHLHAAILAAGKPVE
                     ALS"
     gene            4337..4879
                     /gene="flhC"
                     /locus_tag="PLH85_14030"
     CDS             4337..4879
                     /gene="flhC"
                     /locus_tag="PLH85_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HNB65731.1"
                     /translation="MRNKSVLLEAQDIQKAVELIRLGARMQMLEAETGLSRERLLKLY
                     KEVKGVSPPKGMLPFSTDWFMSWQPNIHASLFMAFHRFVRGNAGLDGLNAIVQAFRLY
                     QEHVESFEMEQVLSLTRAWTLIRFFDARLLQSARCTCCGGEFVTHAYDPTQHYVCGLC
                     HVPSRAGKTRKAQLAESIAP"
     gene            5058..5909
                     /gene="motA"
                     /locus_tag="PLH85_14035"
     CDS             5058..5909
                     /gene="motA"
                     /locus_tag="PLH85_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNB65732.1"
                     /translation="MFLIIGYVIILAASLGTYAVHGSLAALWVPTEYIAIVGLTVGGF
                     VAGNGLGAIKGTVAALPTIFKGSPYSKAFYIDVLCMLYEILAKVRKEGLMSIEGDIEN
                     PEGSPILSKYPAFTADHHAVDFLCDYLRMMVGGNLNAFEIENLMDIEMETHHHEAHGP
                     AHVVSKVADSVPAFGIVVAVMGVVNVMGSVGQPPAVLGKMIGGALVGTFLGILISYGF
                     VAPIAGQLEQKVDEGGKIYQCMKAVLLASMNGYAPQVAVEFGRKVLYSTDRPSFAELE
                     EAIKSRK"
     gene            5959..6957
                     /gene="motB"
                     /locus_tag="PLH85_14040"
     CDS             5959..6957
                     /gene="motB"
                     /locus_tag="PLH85_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNB65733.1"
                     /translation="MSDDTQQPIVVKKIKKGGAGAHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTAQGDLQGIADFFSSPLKVAMAGGSGAGDATSPIVGGGQDLTRSVGQVKRGDV
                     DGKRSMNIEAAKGATRPSGDEGTDAAKKTEQRRERAKLEDLKGQIEAVIEASPSLRPF
                     KNQLLLDITTEGLRIQIVDEQNRPMFDSSSASLKPYTQELLRAIGATLNGVDNTVSLS
                     GHTDSTRYAGGERGFSNWELSANRANASRRELVAGGLAEGKMVRVVGLADTFPLDPAN
                     PAAPINRRISIIVLNKEAEAALRTAGGRKLDVSSESGVDASDIQAGLKPGGRAAPH"
     gene            7108..8316
                     /locus_tag="PLH85_14045"
     CDS             7108..8316
                     /locus_tag="PLH85_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65734.1"
                     /translation="MAQLSPLATVFAGTGWSALVAAGTGFATGDFAAACWAGGLSAVG
                     WGGVLLAGAGRPRDTAAESPRNLDAGQLEPLEAILREASLAIRQQHKGIGAEMHRMQE
                     LLADAIRSLTDSFHGIHAETTAQQSIAVRLTQDNVVDGESSASSFDAFVANTSGVMQR
                     VVDSVIQNSKLGMELVELTDRISRRASDVESILGEITSISKQTNLLALNAAIEAARAG
                     EAGRGFAVVADEVRDLSTRTAQFSQQIGQVMGSMRESVKCTEEAIEKMASTDMTFALE
                     SKQQVEHVLSAMEGINEQRGEAIVRIGEHAQAMDGLVNRAVTALQFQDLVSQLVSRIE
                     QRVRVLDEVLMEVEGLAARIDQSAGTGNPAILHDAVGLLRARLDAMQAASAPASADHP
                     EVSRGEIDLF"
     gene            8339..8704
                     /locus_tag="PLH85_14050"
     CDS             8339..8704
                     /locus_tag="PLH85_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB65735.1"
                     /translation="MAKTVLTVDDSASIRQMVSFTLKSAGYEVVEAVDGMDGLDKAKT
                     RSCNLVLTDQNMPRMDGLSLIKTLRGMPQYRSVPILMLTTESSDTMKSQGRAAGATGW
                     LVKPFDPQKLIEVVKKVIG"
     gene            8742..11042
                     /locus_tag="PLH85_14055"
     CDS             8742..11042
                     /locus_tag="PLH85_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB65736.1"
                     /translation="MAIDMSQFYQVFFEEAAEHLATMVALLLAIDPGAPDPEEVNALF
                     RAAHSIKGSSGTFGFQDMMEVTHILETMLDKVRKGEAPLTDDMVDASLVAGDVLKNLL
                     AAHRGEEEADREAAEAICHRLEALCRALEQGGDAAAPAPLAVPVQTGTGAVGPACFNL
                     HFTLACPPEAMATQLEQLRVELRGLGQVETLRAPDATGDECHLRIQTDLPSDNLRSIL
                     EFVADAASVRVEPQALDAGAEAYGFFAPHDEPVAPATAEPYGFFDPLEPSAPAPQPEP
                     YGLFEDIVPQAAGAQESEAYGFFTELPAPADPIDVAPPSVPSLQADGKEAYGFFEPEP
                     LRVAEPAPESMAAPESAPRPVAVPAAVKKGPPARVGASGDSGSIRVSVEKVDQMINLV
                     GELVITQAMLTQAASNVDPVIFERLINGLAQLERNTRDLQESVMSIRMMPMSVVFSRF
                     PRVVRDLSQKLGKQVELKTIGENTEMDKSLVERITDPLTHLVRNSLDHGLETPDKRRA
                     AGKPETGTITLSAYHQSGNIVIEVGDDGAGLNRARILAKARERGMPVSDAMSDQDVWA
                     LIMEPGFSTAEQVTEVSGRGVGMDVVKKNISAMGGRIELDSMPGVGTRITVRLPLTLA
                     ILDGMSVGVGPETYIIPLGYVMESLQPERGMMKSVSGLERLIQVRGEYLPVVCLHEVF
                     RIHGAITDWSKGIMVVLEADGQSAALFIDQLIGQHQVVIKSLEANYRRVPGIAGATIM
                     GDGHVALILDVPMLVNMARRLMPVAA"
     gene            11179..11682
                     /locus_tag="PLH85_14060"
     CDS             11179..11682
                     /locus_tag="PLH85_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB65737.1"
                     /translation="MLQAAHTAAAGEAEETGFTQELLTFTLGAEEYAIDILKVQEIRG
                     YDTVTSIANAPAFIKGVINLRGTIVPIVDLRIKFSVGKVEYTPFTVVIILNLNGRVVG
                     IVVDAVSDVIALKPEQMRPAPDFAASVDTAYIMGLGALGDRMLIVMDIEKLMLSGEMA
                     LVDEAAH"
     gene            11764..>13508
                     /locus_tag="PLH85_14065"
     CDS             11764..>13508
                     /locus_tag="PLH85_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNB65738.1"
                     /translation="MNRLSVQGKMLLLAVIGLIPIVVLAFMLLARIDADLSFTTKETY
                     TVPMVKPARQLMQAVQTHRAVARQVITGQADAARLEESRARIAEAIRAEDDFQSQHGA
                     MLETDKAWAALRAAWDELKAQSTRVGASESIQLHNAYIARIRDFISHAADQTNATLDP
                     ELETYYLMDIFISRLPELIEDVGSIRALGADVIPRGRTTERERIELNIVVRALRTGID
                     TVNSEVGKAGKEDATVHALLDTPAATFAATVQDYATLVERELLRAEGVTVTYGNVFDS
                     AAKAIDAGYVFFDLSAQDFDRRLAVRMAKLEQARSVAIAVIAVSLGVALYLFLGFRHT
                     MLGAIAEIRNGVARMAGGTLDQNLAVVGQDAFAEIGGAINGMQASLLAKIEADQMTAA
                     SNLRIRNALDKASTNIMLADNDGQIIYCNDSVLKMLRGAESDLRKDLPNFSADGLVGR
                     NFDSFHRNPAHQRNLLSRLSGEHRASIQVGGRTFSLVANPVLNNEGVRLGTVVEWVDR
                     TSEVLVEAELSGLLNAAVSGDFAQRLSMEGKEGFFATLAAGMNQLMGIVATNLEDVAR
                     VLNAIARGDLTEKITA"
BASE COUNT         2433 a         4177 c         4443 g         2455 t
ORIGIN      
        1 cagttcgccc gccaagcggg ccgccgcgta gtcaccgcgc atccgctcaa gcatcctgtc
       61 tagcacgcag aacgtgaaaa gcgacagcaa tcctatgaac gatgcgacag tggatgtatt
      121 aatcctccac ctttcgatcg acaattcgga taatgatgtc aatcttcagg ttgtcaggca
      181 tttccgggca tctcgccact tttttgagtg cgctcgccgc cacgtagcgt tggaacatct
      241 gagagcccct tcttccgaaa tgtcgtgacg ctacatcaac attgccgtgg caatgtccgt
      301 gaattctgat gtttaaagcg cccgattgtt gttatggatg tcctggggtc gggccgcaca
      361 gcatctggtt gttggtggtg tgctcagtct gtcggtgccg agatcatgaa aaatcgctat
      421 gtctggatag ggacctgttc agtgttggcg cacttggtct tgttctggcc ggaacccaat
      481 aggccggggg atggccgacg gccggtcatt tcggtgcgat tgcctgacta tgcccggggt
      541 cagccgccca aggacgaaac gctccttatg gagtcggcgg agactgttga acgggctgtt
      601 ccgatggaga acgtcgcgca tgcgacccct gaggtgccaa acgcctcagg tcgagccagg
      661 acgattccgg cacatagcgt gtcccgcggg tctctggtag atgtatcgcc gaaggcagcc
      721 atcgccacgt ctttgcagac gcttgggcgt gacgggaccg tcgcagaagg gggcgattca
      781 gatgacaggc ccggtctgct gcgttaccgg atcgcactgg cttctgctgc agtgaagcgt
      841 gtgcgagccg agttgtctgt ggtggtgatg caggacgcgg tgcattcgct taccgtcgaa
      901 gtcagcggat tggaggccgg cgtgacggcg gttcgcatca tcgcgtcgtc tggacgggcg
      961 ggcctggatg acatggtctt gcgcgcggtc gaattggcgg ccggagatgt tccgcacccg
     1021 tttgtcgaac gggggaaacc atgggcgttc acgctgcctg tcagtatttc gcaggagttg
     1081 gaagccttgt gaagccatgc ccactgatct tgggcgtacg gggctaccgg agcggcattc
     1141 aggtgcgtaa cggtggatgg atctcgagcg ccgtggtgtg cagccgatag tttcctgatc
     1201 tccgatcttc gaaaaacttc cggagcgtga actccaccga tcggaacgca atttcgttcc
     1261 agggcacgtc cgattcgtcg aacagagccg cttccagggt ttcttcacca ggactgaagt
     1321 ccggagccag tagcgtggcc tgaaagatga tgtggatctg gctgataccc ggaatatcca
     1381 caaagctgaa catggggccc agttctacgc gggcgccagc ttcttcttga gtctcccgca
     1441 ccgcgccctc ggcggcggtt tcgttgtttt ccatgaatcc ggccggaagt gtccagagcc
     1501 cgtagcgtgg ttcgatggcg cggcggcaca gcagcacctg ctcattccac accggcaggg
     1561 cgccgaccac gaccttgggg ttctggtaat ggatcgtccc gcacgcgtca cagacgtggc
     1621 gtggaaggtt gtcgccgggg gggacacgca ccgtcaccgg cgagccgcat tgggagcaga
     1681 agttcatgaa caagcgtgga tcatggggag ccagtcggca ttctagtcga aggtccgtgt
     1741 gctttacacg gccggcggat ggggcacgat cgacttaagg ggatgaggtt tctgtccgat
     1801 aagtcgtgga ctacggccga gtgccgcgtc atcccgtgca aggcaacgat gacttcttcc
     1861 tttgacatca cgaagttcga atatctcaag gcgtcgggcg atctcccttc gccgaagggg
     1921 gttgcactgg cggtcatgcg cgccctgcag cacgacgatt gttcgactgg cgaactggcc
     1981 cgcatcatca agaccgatcc cgcgttcgtc ggacgcatcg tcaaggcggc caatggcgtg
     2041 gcgggcttca atcgtcgggc cgtggcctcg gtgcaggatg cgatcctggt gctcggcatg
     2101 cgggctgtcc gtacactggc cctggggttc tcgctgctgt cagagtaccg caacggtgtg
     2161 tcgcgggagt tcgactatcc gcgctactgg tcgggctgtc tggcacaggc tctggcgatg
     2221 caggccatcg cgcatcacac gcgtgcgggt gcccttgaag acatgttctg tctcggactg
     2281 ctggcgaggg taggcgaatt ggcgctggca accctgtacc cggctgaata cgacacgttg
     2341 cagcgacgcc ggcgcgccga acccgccacg gccatcgcga ggctcgagca tgaattgttt
     2401 gccatctccc acggggaact ggggggcgcc atgctggcgg attggggtct gcccaaggtc
     2461 ttctgcgtgg ccgcggagtt tgctgaacat cctgaacgtg ccgggcatga gccgggatcc
     2521 cgttcggatg tcctgacccg ctcactgcag atggctgcat gcatggctgc gctgtttgtc
     2581 gctccggaga cggagcgggc ccagtatctg ccgcgattga tcgaactggg gggcaccctc
     2641 gatctgccgc cggcgcttgt cggtgagatc agtgacggcg tcgcgcggga gtggcaggcg
     2701 tgggggcccc tgctcgatgt ggagacaccc gacattcctt cattcgaggt gcttgcggcc
     2761 caagcggcgg acatgcgggc gcgcgagatc gagatggcct cggcccctga tgcgaccgag
     2821 acgatcgtgc tgcacgacgc gcagcccgga gcgtcccgcc cgctcggcgt gttggttgtt
     2881 cacgacgatg ccgagacgcg cagtctgatc cggcgggtgc tggaggttgc cgggcatgat
     2941 gttcgagagg ctcgggaagg gcgtcgggcc ctcgagtgcg tgctcgaaag cccgccccag
     3001 ttgttgatcg tggaccgtga tctcgcggat ggggacggac tggagtttgt gcgcaacttg
     3061 cgccagacga agatcgggcg cggattgtat gtgctgatgc tgatgcgtgc cggggatgat
     3121 gaccggctga tagaggcctt cgagaacggt gcggacgact ttgtccacac gccggtcagc
     3181 cccaagatcc tcgctgcacg tctgcgtgcc gcgcagcggg tgatcaatct tcaggaggaa
     3241 ctggagcggg atcgggagga ggtgcgccga ttcgccgcgg agctggcggt ctcgaaccgg
     3301 cgcctccagg aaatggccct gacggattcc ctgaccggct tcccgaaccg ccgctatgcc
     3361 atggagcggg ttgcccagga atggtcgacg gcatcccgca cccgccgtcc cttgtcagcg
     3421 atggtcgttg acgtggatga gttcaagcgc ttcaacgaca actttggcca cgataccggc
     3481 gatacgatcc tgcgtcaggt ggctaattcc ctcaagacgg ccttgcgtgc ccaggatgtg
     3541 gtggcacgga cggggggcga cgagtttctg gtgatctgtc ccgacacgca tctggatgcg
     3601 gccctggcct gtgcagagcg cgtgcgcttt gccgtggagg cgggcggtca tgccagcgca
     3661 ggcggcaggg tgacggtgag catcggtgtg gccactcggg aagcgggaat gaatgatgcc
     3721 gaggcgctcg tcaaacgcgc cgaccaagcc ctttatcagg ccaagaacaa ggggcgcaat
     3781 cgcatcacca ccacccagac cgcacgtccg gcgcccctcg ttccgccccg cgggacccat
     3841 tgaaccgggt acgtttgctt acatctgtag gacaaattct tacacagtcg cagcccgtgg
     3901 gcttacgcga atattcgaag ccgacggatg ttgaatcttg ccggtgaacg gcagaatttg
     3961 ccgcatgaga gtccttattc ttcaaggagt gaatcatgcg caatcacgca ctgcaggatg
     4021 agatccggga actgaatctc gcctatctga tgctcgccca gcagatgctg cgggaggacc
     4081 gggctgtcgc cctctaccgc ctcggcatcg gtgaggacgt ggcggacgtg ctcgaaggtt
     4141 tcagcgcggc ccagctgctg cgcatggcca gttcgaccat gttgctgtgc cagtttcgtt
     4201 tcaacgacag catgctggta gacctcctgg cgtcccatgg tcgggaacgt ggcacggcgc
     4261 acctgcatgc agccatcctc gctgccggca agcctgtcga agccttgtcc tgaagtgcgc
     4321 gcaggaggga cggatcatgc gtaacaagag cgttctgctg gaagcccagg acatccagaa
     4381 ggcggtcgaa ttgatccggc tcggtgcacg catgcagatg ctggaggcgg aaaccggatt
     4441 gagccgcgag cgtctgctca agttgtacaa ggaagtgaag ggggtgtcgc ccccgaaggg
     4501 catgctgccg ttttccacgg actggttcat gtcctggcaa ccgaacatcc acgcctcgtt
     4561 gttcatggcc tttcaccggt tcgtccgtgg caatgccggc ctggacggac tcaacgccat
     4621 cgtccaggcc tttcgtcttt accaggaaca cgtcgaatcc ttcgagatgg agcaggtgct
     4681 gagcctcacc cgcgcctgga cgctgatccg cttctttgat gcccgcctgc tgcagtccgc
     4741 ccgctgcacc tgttgtggcg gcgagttcgt cacgcacgcc tacgatccga cccagcacta
     4801 tgtgtgcggc ctctgtcacg tcccgtcccg cgccggtaag acccgcaagg cgcagcttgc
     4861 cgaatcgatt gctccctgac cctcctcttg cctgagaggt tgtgccgctc gacgctcgcg
     4921 agcggcattt ttttgcggac tgtcctgcgg gacgggtgct caagttcgtt caggcagggc
     4981 cgttattccg accgatggat taacgatctg cgggccgcgc atggaattgc gccggccgcc
     5041 cctgagaggt tctggcgatg tttctgatca tcggttacgt catcattctg gcagcttcgc
     5101 tgggcaccta cgcggtgcac ggcagcctcg cggccctgtg ggtgccgacc gagtacatcg
     5161 cgatcgttgg tctgacggtc ggcgggttcg tggccggcaa cggcctgggt gcgatcaagg
     5221 gcacggtggc ggccctgccg acgatcttca agggttcgcc ctattccaaa gcgttctaca
     5281 tcgacgtcct gtgcatgctc tacgagatcc tcgccaaggt gcgtaaggaa ggcctgatgt
     5341 cgattgaagg ggacatcgaa aatcccgagg ggagcccgat cctgtccaaa tatccggcct
     5401 tcacggcgga tcaccatgcc gtggatttcc tctgcgacta cctgcgcatg atggtgggcg
     5461 gcaatctgaa cgccttcgag atcgagaacc tgatggacat cgaaatggaa acccaccacc
     5521 acgaagccca cgggccggcc cacgtggtgt cgaaagtcgc cgactccgtg ccggccttcg
     5581 gcatcgtcgt ggcggtgatg ggcgtggtca acgtaatggg ctcggtgggc cagccgcccg
     5641 ccgtgctggg taagatgatc ggcggtgcgc tggtggggac tttcctgggg atcctcatct
     5701 cttacggctt cgtcgcaccg atcgccgggc agctcgaaca gaaggtggac gagggcggca
     5761 agatctatca gtgcatgaag gcggtcttgc tggcgagcat gaacggctac gccccgcagg
     5821 tggcggtgga gtttggacgc aaggtgctgt attccaccga tcgccccagc tttgcagagc
     5881 tggaagaggc catcaagagc aggaagtaag cggcatgcgg cggggcaata aacccggcga
     5941 tgcggcggaa tccaggccat gagcgacgat acccaacagc ccattgtcgt caaaaagatc
     6001 aagaagggcg gcgccggtgc ccacggcggt gcctggaaga tcgcctatgc cgacttcgtg
     6061 acggcgatga tggccttctt cctgctgatg tggctgctgg gctccacggc ccagggcgat
     6121 ctgcagggca ttgcggattt cttcagttcg cccctcaagg tggccatggc gggtggctcc
     6181 ggggccggtg acgcgaccag tcccatcgtc ggcggtggcc aggatctgac ccggtcggtg
     6241 gggcaggtga agcgcggcga cgtggacgga aagcgttcca tgaacatcga ggctgccaag
     6301 ggcgcaaccc gcccctccgg cgatgaaggc acggatgcag ccaagaagac cgagcagcgc
     6361 cgcgagcggg ccaagctgga ggacctgaaa ggccagatcg aggccgtcat cgaagccagt
     6421 ccgagcctgc gccccttcaa gaaccaactg ctgcttgaca tcaccaccga aggcctgcgc
     6481 atccagatcg tcgatgaaca gaaccgaccc atgttcgatt cgtccagtgc ctccctcaag
     6541 ccctacaccc aggagctgct gcgcgccatc ggcgccaccc tcaacggcgt ggacaacacc
     6601 gtcagcctgt ccggccatac ggattccacg cggtatgccg gcggcgagcg cggcttttcc
     6661 aattgggagc tgtccgccaa ccgggccaat gcgtcccggc gagaactggt cgccggcggg
     6721 ctggccgaag gcaagatggt gcgggtggtg ggccttgcgg acacctttcc cctcgacccg
     6781 gcgaatcctg ctgcgcccat caaccgacgc atcagcatca tcgtgctcaa caaggaagcc
     6841 gaggctgcct tgcgtaccgc aggcgggcgc aagctcgacg tctccagcga gagcggcgtc
     6901 gatgcgtccg atatccaggc cggcctgaag cccggcgggc gggcggcgcc ccactgacgg
     6961 ttccgtcacg ccgggggccg cgcagcaggc tggggcgtgc atcgccagtc gctgcgggcc
     7021 ttcctggcgt cccgcggaag tgtccgaagc taaagatgcc ggtccgcgtg acgatatgtt
     7081 caccaggaag ttttgctcga attcatcatg gcccagctct ctcccctcgc taccgtgttt
     7141 gccggaaccg gctggagcgc cctcgtcgcg gccggaaccg gctttgcaac cggtgatttt
     7201 gctgccgcct gctgggcagg tggtctcagc gccgtcgggt gggggggcgt gctgctcgct
     7261 ggggccgggc gcccccgcga cactgcggcg gaatcgcccc gcaacctgga tgcgggccag
     7321 ctggagccgc tggaggcgat cctccgcgag gccagcctgg cgattcgaca gcagcacaaa
     7381 ggaatcggtg ccgaaatgca ccggatgcag gaactcctgg ccgatgcgat ccggtcgctg
     7441 actgacagtt tccacggcat ccatgccgag acaactgcgc agcagagcat tgccgtgcgt
     7501 ctcacccagg acaacgtcgt ggacggcgag agcagcgcga gcagcttcga cgccttcgtg
     7561 gccaatacgt caggcgtgat gcagcgggtc gtggacagcg tgatccagaa cagcaagctt
     7621 gggatggaac tcgttgaact gacggaccgg atctccaggc gcgcgagcga tgtggagtcg
     7681 atcctcggcg agatcacgtc catttccaaa cagaccaatc tcctcgccct caacgccgcc
     7741 atcgaggcgg cccgcgccgg cgaggccggt cgaggctttg ccgtggtggc cgacgaggtg
     7801 cgtgatctgt ccacccgcac cgcccagttc agccagcaga tcgggcaggt gatgggctcc
     7861 atgcgcgaaa gcgtgaagtg caccgaggaa gccatcgaga agatggcgtc caccgacatg
     7921 acctttgccc tcgaatccaa gcagcaggtg gagcatgtgc ttagcgcgat ggaggggatc
     7981 aacgagcagc gcggcgaggc gatcgtgcgc atcggcgagc atgcccaggc catggatggc
     8041 ctggtcaatc gcgcggtgac cgcgctccag tttcaggatc tggtgtccca gctggtcagc
     8101 cgtatcgagc agcgcgtacg ggtgctggat gaggttttga tggaggtcga ggggctggcg
     8161 gcccgcatcg accagtcggc cggcacaggc aacccggcca ttttgcacga cgcagtcgga
     8221 ttgctgcgcg cgcgcctcga cgccatgcag gcggcgtccg cgcccgcgtc ggcggatcac
     8281 ccggaggtct cgcgcggcga gattgatttg ttttagcttc aacccctagg aaacagcgat
     8341 ggccaagacc gtactcaccg ttgatgactc tgcctccatc cggcagatgg tctccttcac
     8401 cctgaaaagt gccggctatg aggtcgtcga agcggtcgac ggcatggacg gcctcgacaa
     8461 ggccaagacc cgcagctgca atctggtgct gaccgaccag aacatgccgc gcatggacgg
     8521 cctgtccctc atcaagaccc tgcgcggcat gccccagtac cgttcggtac cgatcctgat
     8581 gctcaccacc gagtcgtccg acaccatgaa gtcccaggga cgtgcggccg gtgccacggg
     8641 ctggctggtc aagcctttcg atccgcagaa gctcatcgaa gtcgtcaaga aagtgatcgg
     8701 ctgagccggt cacgcgacgt cgtcgagtaa gggagacagg catggccatc gacatgagcc
     8761 agttctatca ggtgttcttc gaggaggccg ccgagcatct ggccaccatg gtggccctgc
     8821 tgctggcaat cgatccgggc gcacccgatc cggaggaggt gaacgccctg ttccgggcgg
     8881 cgcattccat caaggggtcg agcggaacct tcggcttcca ggacatgatg gaggtcaccc
     8941 acatcctgga aaccatgctc gacaaggtgc gcaagggcga agcgcccctc accgacgaca
     9001 tggtggatgc gtccctggtg gcaggcgacg tgctcaagaa cctgctcgca gcccatcggg
     9061 gtgaggaaga ggccgaccgg gaggccgcgg aagccatctg tcaccgcctg gaggccctgt
     9121 gccgagcgct cgagcagggt ggggacgccg cggcccctgc gccgctcgcc gtacccgtgc
     9181 agacgggcac cggggccgtg ggaccagcct gtttcaatct ccacttcacc ctggcctgcc
     9241 cgcccgaggc aatggcgacg caactggagc agctgcgggt cgaactgcgc ggactcggcc
     9301 aggtcgagac cttgcgcgcg ccagatgcta ccggtgacga atgtcacctg cgcatccaga
     9361 ccgatctgcc ctcggacaat ctgcgcagca tccttgagtt cgtcgcagat gcagcgtccg
     9421 tgcgcgtcga gccccaggcg ctcgacgcgg gcgcggaggc ttacggattc tttgcgccgc
     9481 acgacgagcc cgtggcgccg gccaccgcgg aaccctacgg gttctttgat ccgctcgaac
     9541 cctcggcccc ggcgccgcaa ccggaaccct acgggttgtt cgaggacatc gttccgcagg
     9601 ccgctggcgc ccaggaaagc gaagcgtacg gcttcttcac ggaactgcct gcgccggccg
     9661 accccatcga tgtggcgccg ccctcagtgc cttcgctgca ggccgacggc aaggaagcgt
     9721 acggattctt cgagcccgag cccctgcggg tcgccgagcc ggccccggag tccatggctg
     9781 cgcccgaatc cgcaccgcgt ccggtggccg ttccggccgc cgtcaaaaag ggtccgcctg
     9841 cgcgggtcgg ggccagtggt gacagcggtt cgatccgcgt cagcgtcgaa aaggtggatc
     9901 agatgatcaa cctggtgggc gaactggtga tcacccaggc catgctcacc caggccgcca
     9961 gcaatgtgga cccggtcatc ttcgagcgcc tcatcaatgg cctcgcccag ctcgagcgca
    10021 acacgcggga tctgcaggaa tcggtcatgt cgatccgcat gatgcccatg tccgtggtat
    10081 tcagccgctt cccgcgggtg gtgcgggacc tgtcccagaa gctgggcaag caggtggagc
    10141 tgaagaccat cggcgagaac accgagatgg acaagagcct ggtggagcgc atcaccgatc
    10201 cgctgaccca cctggtgcgc aacagcctcg accacggcct ggaaacgccc gacaagcggc
    10261 gcgcagccgg caagccggag accggcacga tcaccctgtc ggcgtatcac cagagcggca
    10321 atatcgtcat cgaggtcggg gacgacggcg ccggactcaa ccgcgcccgc atcctcgcca
    10381 aggcccgcga acgggggatg ccggtcagcg acgccatgag cgaccaggac gtctgggcgc
    10441 tgatcatgga gccggggttc tccactgcag agcaggtgac cgaagtgtcc ggccgcggtg
    10501 tggggatgga cgtggtgaag aagaacattt ccgcaatggg cgggcggatc gagctggatt
    10561 ccatgcccgg cgtggggacg cgcatcacgg tgcgcctgcc tctgaccctg gccattctgg
    10621 acggcatgag cgtgggcgtg ggccccgaga cctacatcat cccgctgggc tacgtgatgg
    10681 aatcgctcca gcccgagcgc ggcatgatga agagcgtctc cggcctcgag cggctgatcc
    10741 aggtgcgcgg cgaatacctg ccggtggtgt gtctccacga agtgttccgc atccatggtg
    10801 cgatcactga ttggtcgaaa ggcatcatgg tggtgctgga ggcggacgga cagagcgccg
    10861 cgctgttcat cgaccagctc atcggtcagc accaggtggt catcaagagc ctggaggcca
    10921 attatcgtcg cgtgcccggc attgccggcg ccaccatcat gggcgacgga cacgtggccc
    10981 tgatcctcga cgtgccgatg ctggtgaaca tggcccgacg gctcatgccg gtggcggcct
    11041 gagcgcggac ccgagccgcg tgcacgtccc caaaagccgc ctgcgggcgg cttttgcacg
    11101 tctgccggca ggattcccga tgcttcagaa agcgtggtgc ctgccgtaaa agaagcatcc
    11161 ccttcgagga gtcatcccat gcttcaggct gcccacaccg ccgctgccgg cgaggcggaa
    11221 gaaaccggct ttacccagga actgctcacc ttcaccctcg gtgccgagga gtacgccatc
    11281 gacatcctca aggtgcagga gatccgcggc tacgacacgg tgacctcgat cgccaacgcg
    11341 ccggcgttca tcaagggtgt catcaatctg cgcggcacca tcgtgccgat cgtcgatctg
    11401 cggatcaagt tttccgtggg caaggtggaa tacacgccgt tcacggtggt gatcatcctc
    11461 aacctcaacg gccgggtggt gggcatcgta gtggatgccg tctcggacgt gatcgcgctc
    11521 aaaccggaac agatgcggcc ggcgcctgat ttcgcggcgt cggtggatac cgcctacatc
    11581 atggggctgg gtgccctggg cgatcgcatg ctgatcgtga tggacatcga gaagctgatg
    11641 ttgtccggtg agatggccct ggtggatgaa gcggcccact gagcggcgtc gcccccgaag
    11701 caaccccttc cggattcagg acgaacaaac atggctgcct ttctctctcc cgctgtcggg
    11761 gtcatgaacc gactttccgt gcagggcaag atgctcctgc tggccgtcat cggcctcatc
    11821 cccatcgtcg tgctggcctt catgttgctg gcgcgcatcg acgccgatct cagcttcacc
    11881 accaaggaaa cctacacggt gcccatggtc aagccggccc gccagctgat gcaggcggta
    11941 cagactcatc gggccgtcgc ccgccaggtg atcacgggtc aggccgatgc tgcgcgtctg
    12001 gaggagtccc gcgcgcggat cgccgaggcc atccgcgccg aagacgactt tcagagccag
    12061 cacggggcga tgctcgagac ggacaaggcg tgggcagccc tgcgtgccgc ctgggacgaa
    12121 ctgaaagccc agtccacccg tgtcggggcc agcgaaagca tccagctgca caacgcctac
    12181 atcgcgcgta tccgtgattt catctcccac gcggcggatc agaccaatgc cacgctcgat
    12241 cccgagctgg aaacctatta cctgatggac atcttcatca gccgtctgcc cgagctgatc
    12301 gaggatgtgg gcagcatccg tgcactgggg gccgatgtta ttccccgcgg gcgcaccacg
    12361 gagcgtgagc gcatcgagct gaacatcgtg gtgcgcgcac tccgcacagg gatcgatacg
    12421 gtgaacagcg aggtgggcaa ggccggcaag gaggatgcga ccgtccatgc cctgctcgac
    12481 acgcctgcgg ccacctttgc ggcgaccgtc caggactacg ccaccctggt ggagcgtgaa
    12541 ctgctccgag ccgaaggtgt gaccgtcacc tacggaaatg ttttcgattc cgcagccaag
    12601 gccatcgacg ccggctacgt gttctttgat ctgtccgcgc aggatttcga ccgccgcctg
    12661 gccgtccgga tggccaagct ggagcaggcc cggagcgtgg cgatcgcggt gatcgcggtg
    12721 agcctgggcg tggccctcta cctgttcctc gggttccgcc acaccatgct gggcgccatt
    12781 gccgagatcc gcaacggggt cgcacgcatg gcgggcggca cactcgatca gaatctcgcg
    12841 gtggtcgggc aggatgcatt cgcggagatc ggtggcgcaa tcaatggcat gcaggccagc
    12901 ctcctggcca agatcgaggc tgaccagatg acggctgcca gcaacctgcg catccgcaac
    12961 gccctcgaca aggcgtcaac caacatcatg ctggccgaca acgacggaca gatcatctac
    13021 tgcaacgact cggtcctgaa aatgctgcgg ggcgcggaga gcgatctgcg caaggacctg
    13081 cccaacttca gcgccgacgg actggtcggg cgcaacttcg acagcttcca ccggaatcct
    13141 gcccaccagc gcaacctcct gtcgcgcctg agcggcgagc accgtgcttc catccaggtc
    13201 ggcgggcgaa ccttctcctt ggtcgccaat ccggtgttga acaacgaagg tgtgcgcctg
    13261 ggcaccgtgg tcgaatgggt ggaccggacg tccgaggtgc tcgtggaggc cgagctttcg
    13321 gggctgctca atgccgcggt ctcaggggat ttcgcccagc gcttgtccat ggaaggcaag
    13381 gagggcttct ttgcgacgct cgcggcgggc atgaatcagc tgatggggat cgtggccacc
    13441 aatctggagg acgtggcccg cgtgctcaac gcgattgcgc ggggcgacct gacggagaag
    13501 atcacggc
//
LOCUS       DAOQMM010000101        13471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1927_length_13471_cov_48.486956, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000101 DAOQMM010000000
VERSION     DAOQMM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13471
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_13471_cov_48.486956"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="htpX"
                     /locus_tag="PLH85_14070"
     CDS             complement(<1..806)
                     /gene="htpX"
                     /locus_tag="PLH85_14070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNB65739.1"
                     /translation="MFGNWLKTSILMAGIIALFGVVGGLIGGQQGMLLALVFGGAMNV
                     FSYWFSDKMVLRMYNAQEVDEASSPYLYNMVRELAGRASLPMPRVYIIHEDQPNAFAT
                     GRNPDNAAVAATTGILQMLSARELRGVMAHELAHVQHRDILISTISATLAGAISALAN
                     FAMFFGGRDEEGRPANPIASIAVALLAPLAASLIQMAISRAREFEADRGGAQISGDPN
                     ALADALLKIDAFARGIPMPTAEAHPETGQMMIMNPLSGHGLAGLFSTHPAT"
     gene            complement(925..1848)
                     /gene="fmt"
                     /locus_tag="PLH85_14075"
     CDS             complement(925..1848)
                     /gene="fmt"
                     /locus_tag="PLH85_14075"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNB65740.1"
                     /translation="MKVAFAGTPDFAAIALEAILAAGFEVPLVLTQPDRPAGRGMQLQ
                     PSPVKQVALAAGIPVHQPEKLRTPEQQAPLAACAADVLVVAAYGLILPQAVLDLPRHG
                     CLNIHASLLPRWRGAAPIHRALQAGDAETGITIMQMDAGLDTGPMLLKRAESIQPDDT
                     TASLHDRLAWLGSEMIVEALEALPGGTLTATPQPAEGVTYAAKIGKAEAVVDWTRPAA
                     EIERAIRAFNPFPGAFAPFQGTPVKLWRARAIDARGTPGEVLLAEGAGVIVACGEGAL
                     CITELQKPGGRRLSAADFLRGAPIAAGSRFD"
     gene            complement(1848..2351)
                     /gene="def"
                     /locus_tag="PLH85_14080"
     CDS             complement(1848..2351)
                     /gene="def"
                     /locus_tag="PLH85_14080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB65741.1"
                     /translation="MALLPILRYPDPRLHTRAVPVREVTDEIRTLVANMAETMYEAPG
                     IGLAATQVDVHKRIVVIDVSEDKTGLLALINPEIIERSGEQVCEEGCLSVPGIYDKVS
                     RAEKVRVRALNLQGETFEFDAEGLLAVCVQHELDHLEGKVFVEYLSLLKQTRIKSKLA
                     KKARITA"
     gene            2532..3644
                     /locus_tag="PLH85_14085"
     CDS             2532..3644
                     /locus_tag="PLH85_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNB65742.1"
                     /translation="MRRIISALLVGVAALVPAGAGATDPVRLAEDAPDSHTVVPGDTL
                     WGISGTFLKEPWRWPEVWQLNRDQIRNPHWIYPGQVVVLDRSGANPVLRLGKRIGASP
                     ADRPLYEKRFPQVYSESARDAIPSIPIRVIQPFLVEPLVVEDKDDERAGIVVATQEGR
                     VFTGPGDTIFATRVPPGVPLWNVYRKAQPLKEPVTGEILGYEAAFLGTARVTAEMGVL
                     DADAGVKGEADQPPVPATLQILTVKQEVGKGDRLLPAPKPELLSYVPHAPDADVAGRV
                     VSVYNGVTETGRFNVIAVSLGKRDGMEIGHVLSLHRNRGEAVYREDNVGPAQRYKLPE
                     QRYGLVFIFRVFDRIAYGLVMQSGGVVSVADSVRKP"
     gene            3753..4841
                     /gene="dprA"
                     /locus_tag="PLH85_14090"
     CDS             3753..4841
                     /gene="dprA"
                     /locus_tag="PLH85_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNB65743.1"
                     /translation="MAESHPLAPWLRLTLVPGVGAERQRQLLAAFGPPDAVFAAGHAA
                     LAAIIGSPAARQLLDHDTADAVARSLDWLAQSGNRILTLGDPDYPPALLEIPDPPVLL
                     YIKGDVTLLSRRGIAVVGARSATPQGEANAEAFATALSAAGLPIVSGLALGIDSAAHR
                     GGLGGPGRTVAVIGTGADRIYPARNEALARRIAEAGAIVSELPLGAPPLAHHFPRRNR
                     LIAGLARGVLVVEAAVQSGSLITARLAAECGREVFAIPGSIHSPLARGCHRLIRDGAK
                     LAESAEDVLEELQLEGTMPPAAAASPPDEGDPQAAQVVSALGHDPVDMDTLLQRTSLT
                     SAELYAILLTLELDGRVSRLPGGRVQRL"
     gene            4869..5345
                     /locus_tag="PLH85_14095"
     CDS             4869..5345
                     /locus_tag="PLH85_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNB65744.1"
                     /translation="MFDILVYLFENYVHADACPEPAQLARKLSAAGFEEEEITEALEW
                     LSGLRELSDGDRPAHVARAGSVRIYAPEEQVLLGIECRGFLQFMENAGVLDAECREMI
                     VERALALGDPEIELDNLKVIVLMVLWQQDRPINGLILDELLNESDDEDADAPNVLH"
     gene            5518..8067
                     /locus_tag="PLH85_14100"
     CDS             5518..8067
                     /locus_tag="PLH85_14100"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNB65745.1"
                     /translation="MSKHLIIAEKPSVAADIARAIGGFTKEKDYFESEHYVLSSAVGH
                     LLELSVPAEFEVKRGKWSFAHLPVIPPHFALNPIEKTEDRLKLLTRLIKRKDVTGLIN
                     ACDAGREGELIFSFIAQHAGTHKPVQRLWLQSMTPGAIRDGFAHLRSADDVAGLREAA
                     VCRAESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVEREEKIRAFRPTD
                     YWELEARFGCQAGSYAGRWFDEGFRKPEEGTPLAEHASAYRLWDKTRAEALRAKCQGQ
                     PGSVEEESKPTTQLSPLLFDLTSLQREANGRFGFSARTTLQLAQALYEKHKVLTYPRT
                     DARALPEDYVGQVKGILGGLPDAYAPFANEIVKHGWAKPNKRIFNNAKISDHFAIIPT
                     GTPPKTLSEAEAKIYDLVTRRFLAVFYPAAEYRVTTRITRVAGEAFKTEGKVLVQPGW
                     LAVYGKSAALADEDGGTPQLVAVTPGEQVSTDEVALKALVTKPPARFNEATLLSAMEG
                     AGKMVDDEELRAAMAGRGLGTPATRAQIIENLIGETYILRDGKELVPTAKAFSLITLL
                     RGLGTHELTSPELTGEWEHKLAQMERGALSRSTFMEHIQTMARDMVERAKRYESDTVP
                     GDFATLQTPCPKCGGVVKENYKKFACQGCDWSAWKIVAGRQFEIPEMETLLSTGRVGP
                     LMGFRNKMGRLFNAEIRLNDDQQPEFDFGQPKEDEAAEAVDFSAQEALGTCPKCGGGV
                     YEHGLAYVCEKSVGPGKSCDFRSGKIILQQPIEREQMTKLLADGRTELLKGFVSTRTK
                     RKFSAFLVRGADGKVGFEFEKKEPRAKAGDAADGAAPAAKKAAPRRKPAAG"
     gene            complement(8199..9065)
                     /locus_tag="PLH85_14105"
     CDS             complement(8199..9065)
                     /locus_tag="PLH85_14105"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB65746.1"
                     /translation="MSSPAVQARPALTHDDLDEALQSIQIPACPAVVAEVMREAQKDD
                     PDMRVLARVIASDVGMSAMAIKLANSALFRATSPVKSVSQAVNRLGISNVLSVVIAAA
                     LRSASASLPSELMDPFWNRAATLALSTAVIARCHMGVSAENAYTFALFHDAAIPVMMT
                     HYPQYGAVYAAAQASGRPLVADELAHFPCSHPTVGWLLARNWGLPPVLAAAIRFHHEP
                     DIFSKPDDSLTPEALGMMAVTLVAEHVLAECIGDTGGELDTGLAQQAMAYLGTSDQDL
                     DDFRERVAAAIR"
     gene            9427..11385
                     /locus_tag="PLH85_14110"
     CDS             9427..11385
                     /locus_tag="PLH85_14110"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HNB65747.1"
                     /translation="MTASPSSPAALVSLKHCRDRFVDAIRAAARTARCPEPWLDGLCS
                     AAAEGFEEMAGLRERRGFEQLRGLTASRISLIHQEDLDYSIELINLEQRIHDHVHREM
                     TLADRRLHELLIELNLTRGEDSPFGSEVACLALRALTERERINPVEALDFARQIEAPL
                     KIQLIELYRDVDHTLAGVPSYGQKSGRAPAPRPGTPDGQVLSIGDGGHDPVDGLRWAV
                     ISRAGDGLSETDGQDRAALKAVVDRIEAWMDHERQNGEWAHDELSTSELVQLLPPPRV
                     AALAVVEAVFAHAVTRADLPDPIRALLVELRFPILKQALRHTDLFEGSSRPPALRLVD
                     SIAQLGASLPPEGTVDHSLYRAAAKVVNALVQPDTLPDDAFAVALEALDKRVAERRQT
                     TLERARAFEEKGVQLERREIALHQATRMVSQLLTQAPEGPVRQFLEGYWVQVLTRAAY
                     LRGTDHAEWDSRVRTAAQLIASIVPPADDAERERLRAGLPALRGGLGDGLQAVGLKPE
                     TIDAALAPLDALHAAVLDGRLLPAIPPKPRTPRVHHSPVSGFPGLFALAHKSYLAGEP
                     VLAAEWADIRTGDALVLALPDGGTLRGLVAWIGPGGQLMLIADTDRHDVLAVTFRAFA
                     QQTAQPGSRRLRPFSLVEDAADRRLAHA"
     gene            11473..>13471
                     /locus_tag="PLH85_14115"
     CDS             11473..>13471
                     /locus_tag="PLH85_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNB65748.1"
                     /translation="MCSDPACTAAPPAGSDPDAAIRIRGANQNNLKNLSLDIPLHELV
                     VVTGVSGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPRVDRIDGVPPAIAI
                     DQTNPVRTSRSTVGTMTELADHFKLLYARGARLHCRGCGRPVRRDTPASIHATLAERA
                     AAAGDPRLVVSFPVTVPAAFSEAEIAELLARQGYTRVQARDGDVLHVVQDRFRLGNAE
                     HARVMEALETALRAGHGRLTVHALGDAGEARWPFSAGLHCAECDIAYADPVPSLFSFN
                     SPLGACDTCRGFGRVIGVDFGLVIPDETRTLAEGAVKPWQSPSFKECHDDLVKMAGKY
                     GVPLDVPVADLSPEHRQWLLEGDARWKSWDSSWPRYWYGVRHFFDWLESKAYKMHIRV
                     LLSRYRSYTECPACHGARLKPDALLWHLPAADEALPIHALMARPVDDVATFVTGLALP
                     TPLDEATGLLLDEIRSRLRYLQDVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVN
                     TLFVLDEPSIGLHPRDIGRILGVMERLRDAGNSLVVVEHDPQLMLAADRLLDIGPGPG
                     ERGGRIVAYGPPAQIAADPDSLTGAYLAGRVRVDTRRTPRPVTPAHPRLVLRGARAHN
                     LQGVDAAIPLGRLVGITGVSGSGKSTLIQDVLHPALAKHFGE"
BASE COUNT         2126 a         4925 c         4446 g         1974 t
ORIGIN      
        1 gtggccgggt gggtgctgaa caagcccgcc agcccgtgcc ccgagagggg attcatgatc
       61 atcatctggc cggtctccgg gtgggcctcg gcggtgggca tggggatgcc gcgggcgaag
      121 gcgtcgatct tgagcagcgc gtcggccagg gcgttgggat cgccgctgat ctgcgcgccg
      181 ccccggtcgg cctcgaactc acgggcgcgg gagatggcca tctggatcag gctggcggcc
      241 agcggggcga gcagggccac ggcgatggag gcgatggggt tggccgggcg gccttcctcg
      301 tcacgaccgc cgaagaacat ggcgaagttg gccagcgccg agatcgcacc cgccagggtg
      361 gcggagatcg tggagatcag gatgtcccgg tgctgcacgt gggccagctc gtgggccatc
      421 acgccgcgca gctcgcgggc gctgagcatc tgcaggatgc cggtggtggc cgccacggcg
      481 gcgttgtccg ggttgcggcc ggtggcgaag gcgttgggct ggtcttcgtg gatgatgtac
      541 acgcgcggca tgggcaggct ggcacgaccg gccagttcgc ggaccatgtt gtagaggtag
      601 ggcgaggagg cctcatccac ctcctgcgcg ttgtacatgc gcagcaccat cttgtcggag
      661 aaccagtacg agaacacgtt catggcgccg ccgaagacga gcgcgaggag catgccctgc
      721 tggccgccga tgaggccgcc gacgacaccg aacaaggcga tgatcccggc catcaggatc
      781 gaggtcttga gccagttgcc gaacattgcg ggtatccgtc ctttcgagga tgtggagaaa
      841 gtgacggtta gatgggggag ggtgccgaaa attcaacggc ctgcgtacag gcccgcgcgg
      901 gcgggccggc acccgggcac cgcgtcagtc gaaacggctg cccgccgcga tgggcgcacc
      961 gcgcaggaaa tccgccgccg acagacgccg gccgccgggc ttctgcagct cggtgatgca
     1021 cagggcgcct tcgccgcagg cgacgatcac gccggcccct tcggccagca gcacctcgcc
     1081 gggcgtgccg cgggcgtcga tggcccgcgc ccgccacagc ttgaccggcg tgccctggaa
     1141 gggcgcgaag gcgccgggga aggggttgaa ggcgcggatg gcgcgctcga tctcggcggc
     1201 cgggcgggtc cagtccacca cggcctcggc cttgccgatc ttggcggcgt aggtgacgcc
     1261 ttcggccggc tgcggcgtgg cggtcagcgt gccgccgggc agggcttcga gggcttcgac
     1321 gatcatctcg ctgccgagcc aggcaaggcg gtcatgcagg ctggcggtgg tgtcgtcggg
     1381 ctggatggac tcggcgcgct tgagcagcat ggggccggta tcgaggccgg catccatctg
     1441 catgatggtg atgccggtct ccgcgtcgcc cgcctggagg gcccggtgga tgggcgctgc
     1501 gccgcgccag cggggcagca gcgaggcgtg gatgttgagg cagccgtggc gcggcagatc
     1561 cagcacggcc tggggcagga tgagcccgta ggcggccacc accagcacgt cggcggcgca
     1621 ggccgccagc ggcgcctgct gctcgggcgt gcgcagcttt tccggctgat gcacggggat
     1681 gccggcggcc agggccacct gcttgaccgg gctgggctgg agctgcatgc cgcgcccggc
     1741 cgggcgatcg ggctgggtca ggacgagggg cacctcgaaa ccggcggcga ggatggcctc
     1801 cagcgcgatg gcggcgaaat cgggggttcc ggcgaaggcg actttcatca ggcggtgatc
     1861 cgggccttct tggccagttt gctcttgatg cgggtctgct tgagcaggga caggtattcc
     1921 acgaagacct tgccttccag gtggtcgagt tcgtgctgta cgcacacggc gaggaggccc
     1981 tcggcgtcga attcgaaggt ctcgccctgc agattgaggg cgcgcacgcg gaccttctcg
     2041 gcgcggctca ccttgtcgta gatgccgggc accgacagac acccttcctc acagacctgc
     2101 tcgccggaac gttcgatgat ctccgggttg atgagggcca ggaggccggt cttgtcctcc
     2161 gacacgtcga tgacgacgat gcgcttgtgg acgtccacct gggtggcggc caggccgatg
     2221 ccgggggctt catacatggt ttcggccatg ttggcgacga gggtgcggat ctcgtcggtc
     2281 acctcgcgca ccggcacggc gcgggtatgg agacgggggt ccggataacg gaggatgggt
     2341 agaagggcca taagattgcc gaatgaagac tttacgtgca gaattttgag cgaaatctgc
     2401 gatgcccgcg tccttgtgcc gggccttgcc catcaggaca ccgtccaccc tgcgcagcac
     2461 gggtgggcgc acaccccaga ccgcccggat cgcccggcgg tcaacggccg aaagcgagtg
     2521 cacttccgat gatccgccgc attatatctg ccctcctcgt cggcgtcgcc gccctggttc
     2581 ccgcgggagc cggcgcgacg gatccggtcc gcctggccga ggacgcgccc gacagtcaca
     2641 ccgtggtgcc cggcgacacc ctgtggggca tatccggcac cttcctcaag gagccctggc
     2701 gctggcccga agtgtggcag ctcaaccggg accagattcg caacccccac tggatctacc
     2761 cggggcaggt cgtcgtgctc gaccgcagcg gcgccaaccc cgtgctgcgg ctgggcaagc
     2821 gcatcggcgc cagcccggcc gaccggccgc tctacgagaa gcgcttcccg caggtgtatt
     2881 ccgagtcggc gcgggacgca atcccgagca ttccgatccg cgtcatccag cccttcctgg
     2941 tggagccgct ggtcgtcgag gacaaggatg acgaacgcgc cggcatcgtg gtcgccaccc
     3001 aggaaggtcg tgtgttcacc ggtcccggcg acaccatctt cgcgacgcgc gtcccgccgg
     3061 gggtgccgct ctggaatgtc taccgcaagg cccagcccct caaggagccg gtcaccggcg
     3121 agatcctcgg ctacgaggcc gccttcctcg gcacggcccg cgtgaccgcc gagatgggcg
     3181 tgctcgatgc cgacgccggc gtcaagggcg aggccgacca gccgccagtc cccgccacac
     3241 tgcagatcct cacggtcaag caggaggtcg gcaagggcga ccgcctgctc ccggccccca
     3301 agccggaact gctcagctac gtgccccacg ccccggacgc cgacgtggcg ggccgcgttg
     3361 tctcggtcta caacggcgtc accgagaccg gccgcttcaa cgtgatcgcc gtgagcctgg
     3421 gcaagcgcga cggcatggaa attggccacg tcctgtccct gcaccgcaat cgcggcgaag
     3481 cggtctatcg tgaagacaac gtcggcccgg cccagcgcta caagctgccg gagcagcgtt
     3541 acggcctggt cttcatcttc cgcgtcttcg accggatcgc ttacggtctg gtgatgcaga
     3601 gcggcggcgt ggtcagtgtc gcggacagcg tgcgcaagcc ctgacggccc cgcgccgcgg
     3661 cgaatccgac gcagcggcgc ccgccaaggc accggtccgc accgtcaccg ttcgtcctcc
     3721 caccgcgcag ccctgctccc gtccgtttcc gcgtggccga gtcccatccc ctcgctccct
     3781 ggctgcgtct gacgctggtg cccggtgttg gcgccgaacg ccagcgtcag cttcttgccg
     3841 cctttggtcc cccggacgcc gtcttcgcgg cgggtcacgc ggccctcgct gccatcattg
     3901 gcagccctgc ggcccgccaa ctgctcgatc acgatactgc cgacgcagtc gcccgcagcc
     3961 tcgactggct cgcccagtcg ggcaatcgca tcctcaccct cggcgatccg gactacccgc
     4021 cggcgctgct ggaaattccc gacccgccgg tcctcctcta tataaaaggt gacgtcaccc
     4081 tgttgtccag gcgcggcatc gccgtagtcg gcgcgcgcag cgccacccct cagggcgaag
     4141 ccaatgccga agccttcgcc accgccctgt cggccgccgg tctgcccatc gtcagcggcc
     4201 tcgccctggg catcgacagc gccgcccacc ggggcgggct gggcggtccg gggcgcaccg
     4261 tggccgtgat cgggaccggc gccgaccgga tctatccggc gcgcaatgaa gccctcgccc
     4321 gccggatcgc cgaggccggc gccatcgtca gtgaactgcc cctcggggcg ccgccgctgg
     4381 cccaccattt tccgcgtcgc aacaggctga tcgccggcct ggcccgcggc gtgctggtcg
     4441 tggaagccgc cgtgcagagc gggtcgctga tcaccgcccg cctggccgcc gagtgcggtc
     4501 gcgaggtctt cgcgatcccc ggctccatcc actcgcccct cgcccgcggc tgccatcgcc
     4561 tgatccgcga cggcgccaag ctggccgaat ccgcggaaga cgtgctcgag gagctgcagc
     4621 tcgaaggcac gatgccgcct gccgccgccg cgtcgccccc cgacgagggt gacccgcagg
     4681 ccgcgcaggt cgtgtccgca ctcggccacg accctgtgga tatggacacg ttgctgcaac
     4741 gcaccagctt gacgtcagcg gaactctacg ccattctgct cacgctggaa ctcgatgggc
     4801 gggtttcccg ccttcccgga ggccgcgtac agcgcctcta attcgaccgg cctcaggccc
     4861 cgcgcgccat gttcgacatc ctcgtatatc tgttcgagaa ctacgtccac gccgacgcct
     4921 gccccgaacc cgcccagctc gcgcgcaagc tctctgccgc cggcttcgag gaagaggaga
     4981 tcaccgaggc cctggagtgg ctgtcgggcc tgcgcgaact ctccgacggc gaccgtcctg
     5041 cccacgtggc ccgcgccggc tcggtacgca tctacgcgcc cgaggagcag gtcctgctgg
     5101 gcatcgaatg ccgcggattc ctccagttca tggaaaacgc cggcgtcctc gacgccgagt
     5161 gccgcgagat gatcgtcgag cgtgccctcg ctttgggtga tcccgagatc gagctggaca
     5221 acctcaaggt catcgtgctg atggtgctgt ggcagcagga ccgtcccatc aacggcctga
     5281 tcctcgacga gctcctcaac gagtccgacg acgaggatgc ggacgccccc aacgtcctgc
     5341 actgagccac actccgcttg caagctgcgc gccggcttcc gtatgatgcg gcgcaccccc
     5401 accccaacca tcttgaatag tgcgggttga gcgcttgtgc gccgggctga ccgcccgaac
     5461 gcgcaagggg ttcgttcccg cccttgagag gcaagcgtcg aagagcgcca gaccggcatg
     5521 agcaagcacc tcatcatcgc ggaaaaaccc tccgtcgcgg ccgacatcgc ccgcgccatc
     5581 ggcggtttca ccaaggaaaa ggactacttc gagtcggagc actacgtgct ctcgtcggcc
     5641 gttggccacc tgctcgaact ctccgtcccg gccgaattcg aggtcaagcg gggcaagtgg
     5701 tccttcgccc acctgccggt gattccgccc cacttcgccc tcaaccccat cgagaagacc
     5761 gaggaccgcc tcaagctcct cacccgcctc atcaagcgca aggacgtcac cggcctcatc
     5821 aacgcctgtg acgcgggccg cgagggggaa ctgatcttca gcttcatcgc ccagcacgcc
     5881 ggcacccaca agccggtgca gcgcctgtgg ctgcagtcca tgacgcccgg tgcgatccgc
     5941 gacggctttg cccatctgcg ctcggccgac gacgtggcgg gcctgcgcga ggccgccgtg
     6001 tgccgcgccg agagcgactg gctgatcggc atcaacggca cgcgggcgat gaccgccttc
     6061 aactccaaga ccggcggctt ccacctcacc accgtcggcc gcgtgcagac acccaccctg
     6121 gccatcgtcg tggagcggga ggagaagatc cgcgccttcc ggcccaccga ctactgggag
     6181 ctggaagccc gcttcggctg ccaggccggc agctacgccg ggcgctggtt cgacgagggc
     6241 ttccgcaagc cggaggaggg cacgcccctg gccgagcacg cgtccgccta ccgcctgtgg
     6301 gacaagaccc gcgcagaagc cctccgcgcc aagtgccaag gccagcccgg cagcgtggag
     6361 gaggaatcca agcccaccac ccagctctcg ccgctgctct tcgacctcac ctccctgcag
     6421 cgggaggcca acggccgctt cggcttctcg gcccgcacca ccctgcagct ggcccaggcc
     6481 ctttacgaaa agcacaaggt gctgacctac ccgcggaccg acgcccgcgc cctgcccgaa
     6541 gactacgtcg gccaggtgaa gggcatcctc ggcggcctgc cggacgccta cgcgcccttc
     6601 gccaacgaga tcgtcaagca tggctgggcc aagcccaaca agcggatctt caacaacgcg
     6661 aagatctccg atcacttcgc catcatcccc accggcacac cgcccaagac cctgtcggaa
     6721 gccgaggcca agatctacga tctggtcacc cggcgcttcc tggccgtgtt ctacccggcc
     6781 gccgagtacc gcgtcaccac ccgcatcacc cgtgtggccg gcgaagcctt caagaccgag
     6841 ggcaaggtgc tggtccagcc cggctggctg gcggtgtatg ggaagtcggc ggccctcgcc
     6901 gacgaggacg gcggcacgcc ccagctggtg gccgtcacgc cgggcgagca ggtcagcact
     6961 gacgaggtcg ccctcaaggc tctggtcacc aagccgcccg cgcgcttcaa cgaagccacc
     7021 ctgctgtccg ccatggaagg cgccggcaag atggtggacg acgaggagct gcgcgccgcc
     7081 atggccggcc gcggcctcgg cacgccggcc acccgcgccc agatcatcga gaacctgatc
     7141 ggcgagacct acatcctgcg ggacggcaag gagcttgtcc ccaccgccaa ggccttctcc
     7201 ctgatcacgc tgctgcgcgg cctgggcacc cacgagctga cctcgccgga actcaccggc
     7261 gaatgggagc acaagctggc ccagatggag cgtggcgccc tgtcccgcag caccttcatg
     7321 gagcacatcc agaccatggc gcgggacatg gtcgagcgcg ccaagcgtta cgaatccgac
     7381 accgtgccgg gcgacttcgc gaccctgcag acgccctgtc ccaagtgcgg cggcgtggtg
     7441 aaggagaact acaagaagtt cgcctgccag ggctgcgact ggagcgcctg gaagatcgtc
     7501 gcgggccgcc agttcgagat tcccgagatg gagaccctgc tgtccaccgg gcgtgtcggg
     7561 ccgctgatgg gtttccgcaa caagatgggg cggctcttca atgcggagat ccgcctcaac
     7621 gacgaccagc agccggagtt cgattttggc cagcccaagg aagacgaggc ggccgaagcg
     7681 gtggacttct ccgctcagga ggccctcggc acctgcccca agtgcggtgg cggcgtgtac
     7741 gagcacggcc tcgcctacgt ctgcgaaaaa tccgtgggcc ccggcaagtc ctgcgacttc
     7801 cgctcgggca agatcatcct gcagcagccc atcgagcggg aacagatgac aaaactcctt
     7861 gccgatgggc ggaccgagct gctcaagggc ttcgtgtcca cccgcaccaa gcgcaagttc
     7921 tccgccttcc tggtgcgcgg cgcggacggc aaggtggggt tcgaattcga gaagaaggaa
     7981 ccccgcgcca aggcggggga cgcggcggac ggcgcagcgc ccgccgccaa gaaggcggcc
     8041 cctcgacgca agccggccgc cggctgaacg cacgccgcgc agcatccacg ccgggccgcg
     8101 cggcatgcgc cgcccggccc cgtcacacgt ggatgacccg ccaggtgccg cctgacttca
     8161 cggccatcag gccgccagga acgacggccc gccgcagatc agcggatggc cgcagccacc
     8221 cgctcgcgga aatcgtccag atcctgatca gaggtgccca gataggccat cgcctgctgc
     8281 gcgaggcccg tgtccagctc gccgccggtg tcgccgatgc attctgccaa cacatgctcg
     8341 gccaccaggg tcacggccat catgcccagg gcttcgggcg tcaggctgtc gtcgggcttt
     8401 gaaaaaatat ccggttcgtg gtggaaacgg atcgcggccg ccagcaccgg gggcagaccc
     8461 cagttgcgcg ccagcagcca tccgaccgtc ggatggctgc acgggaagtg ggccagctcg
     8521 tccgccacca gcgggcgccc ggaggcctgc gcggcggcat agacggctcc atactgggga
     8581 tagtgggtca tcatcaccgg gatggcggcg tcgtgaaaca gggcgaaggt ataggcattc
     8641 tcggccgaga cgcccatgtg gcagcgggcg atcacggccg tggaaagggc cagggtggcc
     8701 gcccggttcc agaacgggtc catcagctcg gacggcaggc ttgcactggc cgaccgcaag
     8761 gccgcggcga tcaccacgct cagcacgttg ctgatcccta gccggttgac cgcctgggac
     8821 acgctcttga ccggcgacgt ggcacggaac agggcggaat tggcgagctt gatcgccatg
     8881 gccgacatgc ccacgtcgga ggcgatcacg cgggccagca cgcgcatgtc cgggtcgtcc
     8941 ttctgggcct cgcgcatcac ttcggcgaca acggcagggc aggcggggat ctggatcgac
     9001 tgcagggcct catccaggtc gtcgtgggtc agggcgggcc gggcctgcac ggcaggggaa
     9061 gacatgggaa ctgctcctgt ggggattcgg atcgatcggt tcgaccggtg gttcgtgacg
     9121 ggctgcgcag agggcccgca ccgactccgg catcatggac gccccgtgca cgccgacaca
     9181 gtcggataca agggcggact tctcctgaag aaaggtttcg gccccgcgtg gcatttcttg
     9241 agagtccggt ggcataaaac ttatctcccc gggcattcaa gaatctgata cccgtgaccg
     9301 agaaagacct tgattcatcc atgggattaa agtcgtggtc acccatgccg ttcattgggg
     9361 cgccatgtcg aggaatgtca ccctgcgcag gcccgggcgt cccgtcccgg ttccggagat
     9421 cgagcaatga ccgcatcgcc atcgtcgccc gcagccctgg tctcgctgaa gcactgccgc
     9481 gaccgtttcg tggacgccat ccgggcggcc gcgcgcacgg cccgctgtcc ggaaccctgg
     9541 ctcgacggcc tgtgctccgc ggccgcagag ggtttcgagg aaatggcggg tctgcgcgag
     9601 cggcgcggct ttgaacagct ccgcggcctg acggcgtcac ggatcagcct gatccaccag
     9661 gaagacctcg actactccat cgagctcatc aatctcgagc agcgcatcca cgaccacgtc
     9721 catcgggaga tgaccctcgc ggaccggcgc ctccatgagc tcttgatcga actgaacctc
     9781 acccgcggcg aggattcgcc cttcggcagt gaagtggcct gcctggccct gcgggcgctg
     9841 accgagcggg aacgcatcaa cccggtggaa gccctcgatt tcgcgcgcca gatcgaagcc
     9901 cccctcaaga tccagctcat cgaactctac cgggacgtgg accacaccct cgccggtgtg
     9961 ccgtcctacg gccagaagtc cggtcgcgcg cccgcgccac gccccggcac cccggacggc
    10021 caggtgctga gcatcgggga cggcggccac gatccggtgg acggtctgcg ctgggccgtg
    10081 atctcccggg ccggcgacgg actcagcgag accgacggcc aggaccgcgc cgcgctgaag
    10141 gccgtggtgg atcgcatcga ggcgtggatg gaccacgaac gccagaacgg cgagtgggcc
    10201 cacgacgaac tgagcacctc ggaactcgtg caactcctgc cgccgccccg cgtggcggcc
    10261 ctggccgtgg tggaggcggt gttcgcccac gccgtcaccc gcgccgatct gcccgacccg
    10321 atccgcgccc tgctggtgga attgcgcttc cccatcctca agcaggccct gcgccacacg
    10381 gatctgttcg agggctcctc gcgccctccg gccttgcgcc tggtggacag catcgcccag
    10441 cttggcgcca gcctgccgcc cgaaggtacc gtcgaccact ccctgtaccg cgccgcggcc
    10501 aaggtggtga acgcccttgt ccagcctgac actctgcccg acgacgcctt cgcggtggcc
    10561 ctcgaagccc tcgacaagcg ggtcgcggag cgccgccaga cgaccctgga gcgcgcccgc
    10621 gccttcgaag agaagggcgt tcagctggag cgccgcgaga tcgcgctgca ccaggccaca
    10681 cgcatggtca gccagctcct cacccaggcg ccggaaggcc cggtgcgcca gttcctcgaa
    10741 gggtactggg tccaggtgct gacccgcgcc gcctacctgc gcggcaccga tcacgcggaa
    10801 tgggactcac gggtcagaac ggccgcacag ctcattgcga gcatcgtccc accggccgat
    10861 gacgcggaac gggaacgcct gcgcgccggc ctgccggccc tgcgcggcgg tctgggcgat
    10921 ggcctacagg ccgtcggact caagcccgag accatcgacg ccgccctggc gcccctcgac
    10981 gccctccacg ccgcagtgct cgatggcagg ctcctgcccg ccatcccgcc caaaccacgc
    11041 actccacggg tccaccacag cccggtgagc ggctttccgg ggctgtttgc cctcgcccac
    11101 aagtcctatc tggccggcga accggtgctg gccgccgaat gggccgatat ccgcacgggc
    11161 gacgccctgg tcctcgccct gccggacggc ggcacgctgc gcggcctggt ggcctggatc
    11221 ggccctggcg ggcagctgat gctgatcgcc gacaccgacc gccacgatgt gctggcggtc
    11281 accttccgcg ccttcgccca gcagaccgcc cagccgggca gccggcgcct gcgccccttc
    11341 tccctggtgg aggacgccgc cgaccggcgg ctcgcccacg cctgaccccg gtcggccggg
    11401 aacccggccg cgcggcggcg gtctgcccga gggcgctaca atcgccccgg acaacccagc
    11461 cctcgccctg ccatgtgctc cgatcccgcc tgcacggccg cccccccggc cggctccgat
    11521 cccgacgccg ccatccgcat ccgcggggcg aaccagaaca acctcaagaa cctgtccctg
    11581 gacatccccc tccacgagct cgtggtggtg accggcgtgt ccggttcggg caagagttcc
    11641 ctggtcttcg acaccctcta cgccgaaggc cagcgccgct acgtggagac cttctccccc
    11701 tacgcacgcc agttcctcga ccgcatggac aagccgcggg tggaccgcat cgacggcgtg
    11761 ccgccggcca tcgccatcga ccagaccaac ccggtacgca cctcccgctc caccgtgggc
    11821 accatgaccg agctggccga ccacttcaag ctgctctacg cccgcggcgc acgcctgcac
    11881 tgccgcggct gcggccggcc ggtgcggcgc gacaccccgg ccagcatcca cgccaccctt
    11941 gccgaacggg ccgccgccgc gggcgatccg cgcctggtgg tgagcttccc ggtgaccgtc
    12001 ccggccgcct tcagcgaggc cgagatcgcc gaactcctcg cccgccaggg ctacacccgc
    12061 gtacaggccc gcgacggcga cgtgctccac gtggtgcagg accgcttccg cctgggcaac
    12121 gccgagcacg cccgcgtgat ggaagccctc gaaaccgccc tgcgcgccgg ccacggccgc
    12181 ctcaccgtgc acgccctggg ggacgccggc gaggcgcgct ggcccttcag cgccggcctg
    12241 cactgcgccg agtgcgacat cgcctacgcc gacccggtgc ccagcctgtt ctccttcaac
    12301 tcgcccctcg gcgcctgcga cacctgccgc ggcttcggcc gggtgatcgg cgtcgatttc
    12361 ggcctggtca tcccggacga gaccagaacc ctcgccgaag gcgccgtcaa accctggcag
    12421 agccccagct tcaaggaatg ccacgacgac ctggtgaaga tggccggcaa gtacggcgtg
    12481 cctctggacg tgccggtggc cgacctgtcg ccggaacacc gccagtggct gctggaaggc
    12541 gacgcccgct ggaagagctg ggacagctcg tggccgcgct actggtacgg cgtgcgccac
    12601 ttcttcgact ggctggagag caaggcctac aagatgcaca tccgggtgct gctgtcccgc
    12661 taccgcagct acaccgaatg ccccgcctgc cacggcgccc gcctcaagcc cgacgccctg
    12721 ctgtggcacc tgcccgccgc cgacgaggcg ctgcccattc acgcgctgat ggcccggccg
    12781 gtggacgacg tggccacctt cgtcaccggc cttgcactgc caacacccct tgacgaagcg
    12841 accggcctgc tcctcgacga gatccgcagc cgcctgcgct atctgcagga cgtgggcctc
    12901 ggctacctca ccctggaccg ccagtcgcgc accctgtccg gcggcgaggt gcaacgcatc
    12961 aacctcacca ccgccctcgg cacctcgctg gtgaacaccc tgttcgtcct cgacgagccc
    13021 tccatcgggc tgcacccgcg ggacatcggg cgcatcctgg gggtcatgga gcgcctccgc
    13081 gacgccggca actccctggt cgtggtggag cacgaccccc agctcatgct cgccgccgac
    13141 cgcctcctgg acatcggccc cggccccggt gagcgcggcg ggcgcatcgt cgcctacggc
    13201 cctccggcgc agatcgccgc cgacccggac tccctcaccg gcgcctatct ggccggccgc
    13261 gtccgcgtgg atacccgccg caccccgcgc ccggtcacac cggcgcaccc gcgcctggtc
    13321 ctgcgcggtg cccgcgccca caacctgcag ggcgtggacg cggcgattcc gctcggtcgc
    13381 ctggtgggca tcaccggcgt gtccggctca ggcaaatcca cgctgatcca ggacgtgctc
    13441 catcccgccc tcgccaagca cttcggcgaa c
//
LOCUS       DAOQMM010000102        13415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1942_length_13415_cov_44.501796, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000102 DAOQMM010000000
VERSION     DAOQMM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_13415_cov_44.501796"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLH85_14120"
     CDS             complement(<1..281)
                     /locus_tag="PLH85_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65749.1"
                     /translation="MDWSPPDADGLSSLRLDDPLRVSLKGQRRLRRGAPAPLDVDAWP
                     REERELLAAWVRQAGERPIRHDTLLRAAGPSRAAMADRLVHTLLRAGAV"
     gene            complement(290..3091)
                     /locus_tag="PLH85_14125"
     CDS             complement(290..3091)
                     /locus_tag="PLH85_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB65750.1"
                     /translation="MFHIKQLELVHWDYWRRFTLPLDAQIITIVGPNGSGKTTLLDAL
                     RTLFGLRCSGKRDFRRYVRRAHRSFAWIRAVVANRPGSTGKRPFFPCLKDEVTLACRI
                     RRAGGDWTRDYAIADGDLPIEVLESQQDRAIEWVGYRDYQNRLAWGGLTPAIAKVLAL
                     EQGDTDKLCEYSPKALLELVFDVFGDKEVLDNYAAAREEQLNAEKELAALGLDLDRLR
                     AQAEAKRLEADRFLEWKQLDDEARALAAEIVPRLEAAELVRDIARERADLARGRLERQ
                     HGADELAALQQRLATVEAEKADAEAERRAVRQHDLDAEAAHLTAHDAVRDHEKLLAER
                     SALRTRLAREAGDAAVDLEAAYAKADAHLLHLQRQEREAIAAFEDKTQQLRATREHRG
                     PPAEPEVARFRARLREAGIAHRALAEIVEVTDPGWQSAVEAVLRPYRHVLLLAREQDR
                     HAAWSLGEAERFRHFIVPELEAAPPASAGSLAEVIRFADAVPRWLTDLLNRIQRVEDV
                     DDARRLPRDAEWITRDGYHRERRGARHLGKPADFHFGELARTARLKQLDTDLHSLDAR
                     LRDLRPQVEAAEEEAAGLRARLHGIEAAQLLAARVDDYDRAEDALPDARARLTAAIAA
                     RSEAKGALESLGLRLEGIGIEVDRRKREIGAVTRKLKDLADNAAPRRHEQAQRLLRLR
                     RLRHGMPADWRDPQQTALLAERYGDARGARAEHERLRTRLAEGDWITDDSVLTLAARL
                     KADLGQRERDYAEREAYCAATRRLTADARAAYIAKLRATVRQYGKNLRALGALANVDV
                     DCPVPLLENDDRALAAAGLEVRFDFDRKGAVGLNDGEASGGQQVMKSLILLIALLMDE
                     ARPGGFVFIDEPFAHLDIANIDRVGSFLRATRAQYLITTPVTHNANVFAPAQLTLVTR
                     KKKPGDDWAPPIGLIQRAV"
     gene            complement(3091..3792)
                     /locus_tag="PLH85_14130"
     CDS             complement(3091..3792)
                     /locus_tag="PLH85_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65751.1"
                     /translation="MEHQIRTLTARLLAHRSLPRSDPLVRRALVDDAFRSDLDTRLGA
                     VGLSLIDNPYAAHVAVALAEDMHEPVFGGGREYAASNAGLTRDQLALLVVIWSLIVLP
                     KRERQVNRQRLTDDGQEDMFGKEGPLAHGGEVSGALSEASLLADFAPVLGHKTYVQGK
                     LLSRLAQLGFIERRNGMIHEGPLLDVLLDYRTLADRIIHGTLGEVLAQAGHPPPVRDA
                     FTPDDDDLDDTGEDR"
     gene            complement(3792..5132)
                     /locus_tag="PLH85_14135"
     CDS             complement(3792..5132)
                     /locus_tag="PLH85_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65752.1"
                     /translation="MSEVRLEELLAPGVVADDVPELDTLLRARPLLAAFSTLFHGSEA
                     EVLARLLVLREIGRDAQTTHWTPERLRQRFTYLDPVKLETVLRRLRGNGLLDIGDDNR
                     YTLSDLGRNAVAAVDMLVGFSAGDDLELGFLTAQLAGLQAVGSVTPEALGHLLGKLND
                     LAWHFEDAIASGSEFRIADARRRLSANTRWIEKGTEVLRTLLADPELPFDLARLAQPI
                     GLAQSRLARIDAAFQRAVNKIEAQRVTLGGSGISSSDVAAWLRGRDARALAALARNAL
                     AAAPDLTLLAGGHELLDRAELALSEDARRMEADTALPPPEEAPRRDAPEQEDLRLLEH
                     FSRRLARLAEPGQAPETLARAVAGGGFPAASYRLSLLALLADGGDSDTASPADGPIGD
                     FMRLPVDIRFELATEPVNEDEIATMSAGLVGARGRLPAAEAVPVLDPDTLPRPA"
     gene            complement(5145..6398)
                     /locus_tag="PLH85_14140"
     CDS             complement(5145..6398)
                     /locus_tag="PLH85_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65753.1"
                     /translation="MVRLLAWLSGGLALLLVALAGLLALAVEDHPLVVADDALSPAAI
                     SRARELLRRHDPRKLVRGETRKVDLGADDLASLLRFATRRGLHGQSALRLEQGHAEVR
                     FTAPLPATPLGRYLNLTADLGVSDGRIDIDGVRIGRLPVPAPLFSALFDAYVRHTDLA
                     PELALLRRTVTRVAIAPDALSFVYVWQPELLDSARDLAIGPTRRQRLADAHLRLVARM
                     DAVAARGPRAPLADVLGPLLAGAGEAPAGGERSAAYRDALLVAAFHLSGKDVGAVIPE
                     ARQWPRPRPLTLTLRGREDLAQHFTVSAAVAAAGGEPLANAVGLDKEIDDSDHGSGFS
                     FVDLAADRAGTRLGELAVRNPERLAAALAAGLTDSALLPPIDGLPEAMMAAEFRQRFG
                     GPDAPAYRRMRDEIERRIAALALFR"
     gene            complement(6411..7515)
                     /gene="prfB"
                     /locus_tag="PLH85_14145"
     CDS             complement(join(6411..7433,7435..7515))
                     /gene="prfB"
                     /locus_tag="PLH85_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239085.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNB65754.1"
                     /translation="MEAERLNALSNALADLKARELELRRYLDYDTKAEKLIEVSKQLE
                     DPEVWNDAPRAKELGKEKKTLEGVVLTLDEIDQGIADGRELFELASMEEDEETVQAVE
                     ADVAQLQGKIGELEFRRMFSNPMDANPCFIEIQAGAGGTEAQDWAGMLERMYLRYCER
                     KGFQVELMEESEGEVAGIKSCTIKVGGEYAYGFLRTETGIHRLVRKSPFDSNARRHTS
                     FTSVFVFPEVDDSIEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVQC
                     QNDRSQHRNKDEAWSMLRARLYEAELRKRQSEQQKLEDAKSDIGWGHQIRSYVLDQSR
                     IKDLRTSYEVGNTQAVLDGDLDGFIQASLKQGV"
     gene            complement(7579..8214)
                     /locus_tag="PLH85_14150"
     CDS             complement(7579..8214)
                     /locus_tag="PLH85_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65755.1"
                     /translation="MHVLRLACRLLLVTLLLAAGGARAQALNDTYEGLLLPDTFEQPI
                     PVTVKLTEMGTLLLGQVKTLAPLPGEAALSRAERNNARCLIFAPIAAATHLRMEGTCV
                     PGLFEGNFLISRNGRDTRGTFTLPRKAPAAPSADPADAPRRAAPVDAASPITACLKAN
                     TACLIACPRGDYNVEFLCSNRCRQKFQHCKGKSTLQTPAQPATGSTTPSAN"
     gene            complement(8248..9000)
                     /locus_tag="PLH85_14155"
     CDS             complement(8248..9000)
                     /locus_tag="PLH85_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65756.1"
                     /translation="MPDPAPRLHDCLADASGGLRFHLRAWRHRRRWAAFHAAVADWLR
                     AWHPDRPHLVLVGPSAGYDLPAGWLGGFTRITVLEPDPLARWLLRRRPDATRLHFDDL
                     DCLATPDGLAHLKARFPDAAILFCNVLGQVTAPGGRGWAELCACHLRDLPWASLHDVV
                     STPRAPRADAVRASFPGGTSLEAVLAAFWPGGELPLVDHATFALGGDVPHHYALWPLR
                     PGRWHLVEWVAHAMQNDTSAMPDDAAGRGRAV"
     gene            complement(9061..9993)
                     /gene="secF"
                     /locus_tag="PLH85_14160"
     CDS             complement(9061..9993)
                     /gene="secF"
                     /locus_tag="PLH85_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNB65757.1"
                     /translation="MEFFRIHKDIPFMKHALVFNVISLITFLLAVFFLVTKGLHFSVE
                     FTGGTLMEVAYTEPPTLERLRDALAHEGYADAQVQNFGSARDVLIRLPNREGMETAKV
                     SEKVMATLAAVEGPKAELRRVEFVGPQVGKELASDGAMALLLVIGGIVLYLALRFEWR
                     FAVSAIVANLHDVVIILGFFALFQWEFSLPVLAATLAVLGYSVNESVVVFDRVRETFK
                     KKRGLTTPEVLDHAITSTISRTIITHGCTQIMVLSMLLFGGETLYYFALALTIGICFG
                     IYSSVLVASPLVMWLGVSREQFIQVKKDKQEAVV"
     gene            complement(10027..11886)
                     /gene="secD"
                     /locus_tag="PLH85_14165"
     CDS             complement(10027..11886)
                     /gene="secD"
                     /locus_tag="PLH85_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNB65758.1"
                     /translation="MNRYPLWKNLLIGLVLLWGLLYTLPNFYGEVPAVQVSSGKATLK
                     LDATATLKRVEDTLHGAGLQHDGLFADLTSVRARFHDTDTQLKAKDAIEKAFNPDPAD
                     PSYVVALNLLSASPQWLTAIHALPMYLGLDLRGGVHFLLQVDMKGAVTKRLDATAGDL
                     RTLLRDKSVRHAGISREGQSVAIRFRDAETLARGRAVITASTNDLLLSEGQDGADFKL
                     TATLNPLAQKNIQESAIRQNIVTLHNRINELGVAEPVIQQQGGDRIVVQLPGVQDVAK
                     AKDILGRTATLEIRMVDDAPGALEAALNGSIPPGSELYSERGGSPLLVKKQVVLTGER
                     LTDAQPGFDGQTHEPAVHLTLDAAGARIFKDVTRENVNKRMAILLIEKGKGEVITAPV
                     IRGEIGGGRVQISGRMSSEEATDTALLLRAGSLAAPMEIVEERTIGPSLGADNIRKGF
                     HSTLWGFVAIAAFMCLYYMLFGLVSVLALGSNLLLLVALLSLLQATLTLPGIAAIALT
                     LGMAIDANVLINERIREELRNGSSPQAAISAGYERAFDTILDSNVTTLIAGIALLVFG
                     SGPVRGFAVVHCLGILTSMFSAILVSRGLVNLIYGKKRKLEKLAIGQVWKPGL"
     gene            complement(11955..12287)
                     /gene="yajC"
                     /locus_tag="PLH85_14170"
     CDS             complement(11955..12287)
                     /gene="yajC"
                     /locus_tag="PLH85_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNB65759.1"
                     /translation="MLISNAFAQAAPAAAQDPTAGYGQIVIMVAMFAIFWFMLIRPQQ
                     KRAKEHKALIEGLQKGDEVVTQGGLVGKVAQVGDNYVHLEIAANTQVVVQKPAIGIVL
                     PKGTLKSL"
     gene            complement(12485..12811)
                     /locus_tag="PLH85_14175"
     CDS             complement(12485..12811)
                     /locus_tag="PLH85_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65760.1"
                     /translation="MKSPKILPWYARKAGVSVERAEALWRKAVREATADTGWVGTSEF
                     WGAALDRFLELLQDEQTTLCQPHMETFVRSQHRMWLLPLLAAEDMFNAMSANWQRYRN
                     QMRKAA"
     gene            13120..>13415
                     /locus_tag="PLH85_14180"
     CDS             13120..>13415
                     /locus_tag="PLH85_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB65761.1"
                     /translation="MSQLLEHLNAEQLAAVTLPPRHALILAGAGSGKTRVLTTRIAWL
                     IQNGHCSPQGILAVTFTNKAAKEMLTRLSALLPINTRGMWIGTFHGLCNRFLRA"
BASE COUNT         1914 a         3942 c         5391 g         2168 t
ORIGIN      
        1 tcgacggcgc cggcccgcag cagggtgtgt acgaggcggt cggccatggc cgcgcgggaa
       61 gggccggcgg cgcgcagcag ggtgtcgtga cggatcgggc gttcgcccgc ctggcgcacc
      121 caggcggcga ggagttcgcg ttcttcgcgg ggccaggcat ccacgtcgag gggcgccggg
      181 gcgccgcgac gcaggcggcg ctggcccttg aggctgaccc gcagggggtc gtccaggcgc
      241 aggctggaga gtccgtcggc gtcgggcggg ctccagtcca tggctgtgct cagaccgccc
      301 gctggatcag gccgatgggc ggcgcccagt cgtcgccggg tttcttcttg cgggtgacca
      361 gggtgagctg ggccggggcg aagacgttgg cgttgtgggt gaccggggtg gtgatgaggt
      421 actgggcgcg ggtggcgcgc aggaagctgc cgacccggtc gatgttggcg atgtccaggt
      481 gggcgaaggg ttcgtcgatg aagacaaagc cgccgggacg ggcctcgtcc atgagcaggg
      541 cgatgagcag gatcagggac ttcatgacct gctggccgcc ggaggcttcg ccgtcgttga
      601 ggccgacggc gcccttgcga tcgaagtcga agcgcacttc gaggccggcg gcggcgaggg
      661 cgcggtcgtc gttctccagc aggggcaccg ggcagtccac gtccacgttg gcgagcgcgc
      721 cgagggcgcg cagattcttg ccgtactggc gcacggtggc gcgcagtttg gcgatgtagg
      781 cggcgcgggc gtcggcggtg aggcgacggg tggcggcgca gtaggcttcg cgctcggcgt
      841 agtcccgctc gcgctggccc aggtcggctt tgaggcgggc ggcgagggtg agcacgctgt
      901 cgtcggtgat ccagtcgccc tcggcgaggc gggtgcgcag gcgttcgtgc tcggcgcggg
      961 cgccgcgggc gtcgccgtag cgttcggcga gcagggcggt ctgctgcggg tcgcgccagt
     1021 cggcaggcat gccgtggcgc aggcggcgca ggcgcaggag gcgctgggcc tgttcgtgac
     1081 ggcgcggggc ggcgttgtcg gcgaggtcct tgagcttgcg ggtgacggcg ccgatctccc
     1141 gtttgcgccg gtccacctcg atgccgatgc cttcgaggcg caggccgagg ctttccagcg
     1201 cgcccttggc ctcgctgcgg gcggcgatgg cggcggtgag gcgggcgcgg gcgtcgggca
     1261 gggcgtcctc ggcgcggtcg taatcgtcga cgcgggcggc gaggagctgg gcggcctcga
     1321 tgccgtgcag acgggcgcgc aggccggcgg cttcttcctc ggcggcctcg acctggggac
     1381 gcaggtcgcg caggcgggcg tcgaggctgt gcaggtcggt gtcgagctgc ttgaggcggg
     1441 cggtgcgggc cagttcgccg aagtggaaat cggcgggttt gcccaggtga cgggcgccgc
     1501 ggcgttcccg gtggtagccg tcgcgggtga tccattcggc gtcacggggc aggcggcggg
     1561 cgtcgtccac gtcttcgacg cgctggatgc ggttgagcag atcggtgagc cagcgcggca
     1621 cggcgtcggc aaagcggatg acttcggcga ggctgccggc gctggcgggc ggggcggctt
     1681 ccagctcggg cacgatgaag tggcggaagc gctcggcttc gcccagggac caggcggcgt
     1741 gacggtcctg ctcgcgcgcc agcagtagga cgtgccgata ggggcgcagg acggcctcga
     1801 cggcggactg ccagccggga tcggtgactt cgacgatctc ggcgagggcc cggtgggcga
     1861 tgccggcttc ccgcaggcgc gcgcggaagc gggcgacttc gggttcggcg ggtgggccgc
     1921 ggtgctcgcg ggtggcgcgc agctgctggg tcttgtcctc gaaggcggcg atggcctcgc
     1981 gttcctggcg ttgcagatgg agcaggtggg cgtcggcctt ggcgtaggcg gcttccaggt
     2041 ccacggcggc gtcgccggcc tcgcgtgcga gccgggtgcg cagggcgctg cgttcggcga
     2101 ggagcttttc gtggtcgcgc acggcgtcgt gggccgtcag gtgggcggct tcggcgtcca
     2161 gatcgtgctg gcgcacggcg cggcgttcgg cttcggcgtc ggccttttcg gcttcgacgg
     2221 tggcgaggcg ctgctgcagc gcggccagtt cgtcggcgcc gtgctggcgt tcgaggcggc
     2281 cacgggccag gtcggcgcgc tcgcgggcga tgtcacggac cagctcggcg gcttccaggc
     2341 ggggcacgat ctcggcggcg agggcgcggg cttcgtcgtc gagctgcttc cattcgagga
     2401 agcggtcggc ttcgaggcgt ttggcttcgg cctgggcgcg caggcggtcc aggtcgaggc
     2461 cgagggcggc gagttccttc tccgcgttga gctgctcttc gcgggcggcg gcgtagttgt
     2521 ccagcacttc cttgtcgccg aaaacgtcga agaccagctc cagcagggcc ttgggcgagt
     2581 attcgcagag cttgtcggtg tcgccctgct ccagagcgag gaccttggcg atggccgggg
     2641 tgaggccgcc ccaggcgagg cggttctggt agtcccggta gccgacccat tcgatggccc
     2701 tgtcctgctg ggattcgagg acttcgatgg gtagatcgcc gtcggcgatg gcgtagtcgc
     2761 gggtccagtc tccgccggcg cggcggatgc ggcaggcgag ggtgacttcg tccttgaggc
     2821 agggaaagaa ggggcgcttg ccggtgctgc cggggcggtt ggcgacgacc gcgcggatcc
     2881 acgcgaagct gcggtgggcg cgccgcacgt agcggcggaa gtcgcgcttg ccggagcagc
     2941 gcaggccgaa gagggtgcgc agggcgtcga gcagggtggt cttgccggag ccgttggggc
     3001 cgacgatggt gatgatctgg gcgtcgaggg gcagggtgaa gcgccgccag tagtcccagt
     3061 ggaccagttc gagctgcttg atgtggaaca tcagcggtcc tccccggtgt cgtccaggtc
     3121 gtcgtcgtcg ggcgtgaagg cgtcccgcac ggggggcggg tggccggcct gggcgaggac
     3181 ttcgccgagg gtgccgtgga tgatgcggtc ggcgagggtg cggtagtcga ggagcacgtc
     3241 gaggagcggg ccttcgtgga tcatgccgtt gcgccgctcg atgaagccga gctgcgccag
     3301 gcgggacagg agcttgccct gcacataggt cttgtggccg aggaccgggg cgaagtcggc
     3361 cagcagggag gcttcggaga gggcgccgga gacttcgccg ccgtgggcca gggggccttc
     3421 cttgccgaac atgtcttcct gaccgtcgtc ggtgaggcgc tggcgattaa cctggcgctc
     3481 ccgcttgggc aggacgatga gggaccagat gaccaccagc agggcgagct ggtcgcgggt
     3541 gaggccggcg ttgctggcgg cgtattcgcg gccgccgccg aagacgggtt cgtgcatgtc
     3601 ctcggcgagg gcgacggcca cgtgggcggc gtaggggttg tcgatgaggc tgaggccgac
     3661 ggcgcccagg cgggtgtcca ggtcgctgcg gaaggcgtcg tccaccaggg cgcggcgcac
     3721 cagcgggtcg ctgcggggca ggctgcggtg ggccagcaga cgggcggtga gggtgcggat
     3781 ctggtgttcc atcaggcagg tctgggcagg gtgtcggggt cgaggacggg gacggcttcg
     3841 gcggcgggca gacggccgcg ggcgccgacc aggccggcgc tcatggtggc gatctcgtct
     3901 tcgttgacgg gctcggtggc gagctcgaag cggatgtcga ccggcaggcg catgaagtcg
     3961 ccgatggggc cgtcggcggg gctggcggtg tcgctgtcgc cgccatcggc gaggagggcg
     4021 aggagggaca ggcggtagga ggcggccggg aatccgccgc cggcgacggc gcgggcgagg
     4081 gtctcgggcg cctggccggg ctcggcgagg cgggccaggc ggcggctgaa gtgctccaac
     4141 aggcgcaggt cttcctgttc cggggcgtcc cggcgcgggg cttcttcggg cggcggcagg
     4201 gcggtgtcgg cttccatgcg gcgggcgtct tcggagaggg ccagctcggc ccggtcgagg
     4261 agctcgtggc cgccggcgag cagggtgagg tccggtgcgg ccgccagggc gttgcgggcg
     4321 agggcggcca gggcacgggc gtcgcggccg cgcagccagg cggccacgtc gctggaggag
     4381 atgccggagc cgccgagggt gacgcgctgg gcctcgatct tgttgacggc gcgctggaag
     4441 gcggcgtcga tgcgcgccag gcgcgactgg gcgaggccga tgggctgggc caggcgggcc
     4501 aggtcgaagg gcagctcggg gtcggcgagg agggtgcgca ggacttcggt gcccttttcg
     4561 atccagcggg tgttggcgga caggcgccgg cgggcgtcgg cgatgcgaaa ttcggagccg
     4621 gaggcgatgg cgtcctcgaa gtgccaggcg aggtcgttga gcttgccgag caggtggccg
     4681 agggcttccg gagtgacgct gccgacggcc tgcaggccgg cgagctgggc ggtcaggaag
     4741 cccagttcca ggtcgtcgcc ggcgctgaag ccgaccagca tgtccaccgc ggcgacggca
     4801 ttgcggccca ggtcggagag ggtgtaacgg ttgtcgtcgc cgatgtcgag gaggccgttg
     4861 ccgcgcaggc ggcggaggac ggtttcgagc ttgaccgggt cgaggtaggt gaagcgctgg
     4921 cgcaggcgct cgggcgtcca gtgggtggtc tgggcgtcgc gaccgatctc gcgcagcacc
     4981 agcaggcggg ccagcacttc ggcctcgctg ccgtggaaga gggtgctgaa ggcggcgagc
     5041 aggggacgtg cgcgcagcag ggtgtcgagc tccggcacgt cgtcggcgac gacacccggc
     5101 gcgaggagct cttcgaggcg gacttcgctc atggggacga cgcgtcagcg gaacagggcg
     5161 agggcggcga tgcgccgttc gatctcgtcg cgcatgcgtc ggtaggcagg ggcgtcgggg
     5221 ccgccgaagc gctggcggaa ttcggcggcc atcatggctt cgggcaggcc gtcgatgggg
     5281 ggcagcaagg cgctgtcggt gaggccggcg gccagagcgg cggcgagacg ttcgggattg
     5341 cgtacggcca gctcgcccag gcgggtgccg gcccggtcgg cggcgagatc cacgaaggag
     5401 aagccgctgc cgtggtcgga gtcgtcgatt tccttgtcga ggccgaccgc gttggcgagg
     5461 ggctcgccac ccgccgcggc gacggcggcg gagactgtga agtgctgggc gagatcctcg
     5521 cggccacgca gggtgagggt cagcggacgc ggtcgcggcc actggcgcgc ttcgggaatc
     5581 acggcgccca cgtccttgcc ggacaggtgg aaggcggcca ccagcagggc gtcccggtag
     5641 gcagcactgc gctcgccacc agcgggggcc tcgccggcgc cggcgaggag cgggccgagc
     5701 acgtcggcca ggggcgcgcg gggaccgcgg gcggcgacgg catccatgcg ggcgacgagg
     5761 cgcaggtggg cgtcggcgag gcgctgacgg cgggtcgggc cgatggccag gtcgcgggcg
     5821 ctgtcaagga gctccggttg ccacacgtag acgaagctga gagcgtcggg ggcaatggcc
     5881 acacgggtga cggtgcggcg caggagggcg agctccggcg ccagatcggt gtggcggacg
     5941 taggcgtcga acagcgcaga gaacagcggt gcggggacgg ggaggcggcc gatgcgcacg
     6001 ccgtcgatgt cgatgcggcc gtcgctcacg cccaggtcgg cggtcaggtt gaggtagcgc
     6061 cccagaggcg tggcggggag gggcgcggtg aagcggacct ccgcgtgtcc ctgttcgagg
     6121 cggagggcgc tctgcccatg caggccgcgg cgggtggcga agcgcaggag gctcgccaga
     6181 tcgtcggcgc cgagatccac cttgcgggtc tcgccccgga ccagcttgcg cgggtcatgg
     6241 cggcgcagga gctcccgcgc gcggctgatc gcggcagggg acagggcgtc atcggcgacg
     6301 acgaggggat ggtcctccac cgccagcgcg agcaggccgg cgagcgcgac gaggagcagc
     6361 gcgaggccgc cggacagcca ggcgaggagg cggaccatgc gcggggggac tcagacgccc
     6421 tgcttgaggc tggcctggat gaagccgtcc aggtcgccgt cgagcacggc ctgggtgttg
     6481 cccacttcgt agctggtgcg cagatccttg atgcggctct ggtccagcac gtaggagcgg
     6541 atctggtggc cccagccgat gtcgctcttg gcatcctcga gcttctgctg ctcgctctgg
     6601 cgcttgcgca gctcggcttc gtagaggcgc gcgcgcagca tcgaccaggc ttcgtccttg
     6661 ttgcggtgct gggaacggtc gttctggcac tgcacgacga tgccggtagg gatgtgggtg
     6721 agacgcaccg cggagtcggt cttgttgatg tgctggccgc cggcgccgga ggcgcggtag
     6781 gtgtcggtgc gcacgtcggc cgggttgatg tcgatctcga tggagtcgtc cacctcgggg
     6841 aagacgaaca ccgaggtgaa gctggtgtgg cggcgggcgt tggagtcgaa ggggctcttg
     6901 cgcacgaggc ggtggatgcc cgtttcggtg cgcaggaagc cgtaggcgta ttcgccgccc
     6961 accttgatgg tgcagctctt gatgccggcc acttcgcctt cggactcttc catcagctcg
     7021 acctggaagc ccttgcgttc gcagtagcgc aggtacatgc gttcgagcat gccggcccag
     7081 tcctgggctt cggtgccgcc ggcgccggcc tggatttcga tgaagcaggg gttggcgtcc
     7141 atcggattgc tgaacatccg gcggaattcc agctcgccga tcttgccctg gagctgggcg
     7201 acgtcggctt cgacggcctg gaccgtctct tcgtcctctt ccatggaggc gagctcgaag
     7261 agttcgcgac cgtcggcgat gccctggtcg atttcatcca gggtgaggac gacgccttcg
     7321 agggtcttct tttccttgcc gagttccttg gcgcggggcg cgtcgttcca gacctcgggg
     7381 tcttcgagct gcttgctgac ttcgatcagc ttttcggcct tggtatcgta gtcaaagata
     7441 cctccgcagt tcgagctcgc gggccttgag gtcggcgagg gcgttggaga gggcgttgag
     7501 gcgttcggct tccatgttcg gggaccctgg gaatgcggga aaaccctgca ttataccttc
     7561 gggggcgccc gaacggggtc agttggccga cggggtggtg ctgccggtgg cgggttgggc
     7621 gggcgtctgg agggtgctct tgcccttgca gtgctggaat ttctggcggc agcggttgga
     7681 gcagaggaat tccacgttgt aatcgccccg cggacaggcg atgaggcagg cggtgttggc
     7741 cttgaggcag gcggtgatcg gggacgcggc gtccacgggg gccgcgcggc gcggcgcatc
     7801 ggcgggatcg gcgctcgggg cggccggggc cttgcgtggc agggtgaagg tgccgcgggt
     7861 gtcacgcccg ttgcgggaga tcaggaagtt gccttcgaag aggccgggaa cgcaggtgcc
     7921 ttccatgcgc aggtgggtgg ctgcggcgat gggggcgaag atcaggcagc gggcgttgtt
     7981 gcgttcggcg cgggacaggg cggcctcgcc gggcagcggg gcgagggtct tgacctggcc
     8041 gaggaggagg gtgcccatct cggtgagctt gacggtgacc gggatgggct gctcgaaggt
     8101 gtcgggcagg agcaggcctt cataggtgtc gttgagcgcc tgggcccgtg cgccgcccgc
     8161 ggcgagcagc agggtgacga ggagcaggcg gcaggcgagt cgaaggacgt gcatggtgga
     8221 tcgctgggcg aaatcggccg gacgattcta cacggcccgt ccacggccgg ccgcgtcgtc
     8281 cggcatggcc gacgtgtcat tttgcatggc gtgggcgacc cattccacca ggtgccagcg
     8341 accgggccgc agtggccaca gggcgtagtg gtgagggaca tcgccgccca gggcgaaggt
     8401 ggcgtgatcc acgaggggca gctcgccccc cggccagaag gcggcgagca cggcctcgag
     8461 ggaggttccg cccggaaacg atgcgcggac cgcgtcggca cggggcgcgc gaggggtgga
     8521 gaccacatcg tggaggctgg cccagggcag gtcgcgcagg tggcaggcgc acagttcggc
     8581 ccagccccgg ccgccgggtg cggtgacctg gccgaggaca ttgcagaaga ggatggcggc
     8641 gtccgggaag cgggctttca ggtgggccag accgtcgggg gtggcgaggc agtccaggtc
     8701 gtcgaaatgc agccgggttg cgtcggggcg acggcgcagc agccagcggg cgagagggtc
     8761 gggctccagc accgtgatgc gtgtgaatcc gcccagccag ccggccggca gatcgtagcc
     8821 ggcgctgggg ccgacgagga cgaggtgggg ccggtcagga tgccaggcgc gcaaccagtc
     8881 ggccaccgcg gcgtggaatg cggcccagcg gcgccgatgg cgccaggccc gcaggtggaa
     8941 gcgcagtcca ccgctggcgt cggccaggca gtcgtgcagg cggggcgcgg gatcgggcat
     9001 cggcggctca aatgaaacgg gccgcacagg ggcggcccgg caggctggcg cgcggcgcgc
     9061 tcagaccacc gcttcctgct tgtccttctt gacctggatg aactgttcgc gggagacgcc
     9121 cagccacatc accagcgggc tggcgaccag caccgaggag tagatgccga agcagatgcc
     9181 gatggtcagg gcgagggcga agtagtagag ggtctcgcca ccgaacagga gcatggacag
     9241 caccatgatc tgggtgcagc cgtgggtgat gatggtccgg ctgatggtgc tggtgatggc
     9301 gtggtcgagc acctcgggtg tggtgaggcc acgcttcttt ttgaaggttt cgcggacccg
     9361 gtcgaagacc accaccgatt cgttcaccga atagcccagc acggcgaggg tggcggccag
     9421 caccggcagg gagaattccc actggaacaa ggcgaagaag cccaggatga tgaccacgtc
     9481 gtggaggttg gcgacgatgg ccgacacggc gaagcgccac tcgaagcgca gggccaggta
     9541 gaggacgatg ccgccgatca ccagcagcag ggccatggcg ccgtcggagg cgagttcctt
     9601 gcccacttgc gggccgacga actccacccg gcgcagctcg gccttggggc cttccacggc
     9661 ggcgagggtg gccatgacct tttcgctgac cttggcggtc tccatgcctt cccggttggg
     9721 caggcggatc aacacgtcgc gggcgctgcc aaagttctgc acctgggcgt cggcatagcc
     9781 ttcgtgggcg agggcgtcgc gcaggcgctc gagggtcggt ggctcggtgt aggccacctc
     9841 catgagggtg ccgccggtga attcgacgga gaagtgcagg cccttggtga ccaggaagaa
     9901 caccgccagc aggaaggtga tcaacgaaat cacgttgaac accagcgcat gcttcatgaa
     9961 cgggatgtct ttgtggatgc ggaagaattc catgatggga gtccgattcg atgaggcgcg
    10021 cggggcttac aggccgggtt tccagacctg gccgatggcc agcttctcga gcttgcgctt
    10081 cttgccgtag atcaggttga ccaggccgcg ggagaccagg atggcgctga acatggaggt
    10141 caggatgccc aggcagtgca ccaccgcaaa gccgcgtacc gggccgctgc cgaagacgag
    10201 cagggcgatg ccggcgatca gggtggtgac gttggagtcg agaatggtgt cgaaggcgcg
    10261 ctcgtagccg gcggagatgg ctgcctgggg gctggagccg ttgcgcagct cttcgcggat
    10321 acgttcgttg atgagcacgt tggcgtcgat ggccatgccc agggtgaggg cgatggcggc
    10381 gatgcccggc agggtcaggg tggcctgcag cagggacagc agggccacca gcaggagcag
    10441 gttggacccc agggccagca ccgacaccag gccgaacagc atgtaataca ggcacatgaa
    10501 ggcggcgatg gcgacgaagc cccacagggt ggagtggaag cccttgcgga tgttgtcggc
    10561 gcccaggctg gggccgatgg tgcgctcctc gacgatctcc atcggcgcgg cgagggagcc
    10621 ggcgcgcagc agcagagcgg tgtcggtggc ttcttcggag ctcatgcgcc cggagatctg
    10681 cacccggccg ccgccgatct cgccgcggat gaccggcgcc gtgatgactt cgcccttgcc
    10741 cttttcgatg agcaggatgg ccatgcgctt gttgacgttc tcgcgggtga cgtccttgaa
    10801 gatgcgggcg ccggccgcgt cgagggtcag gtggacggcg ggctcgtggg tctggccgtc
    10861 gaagccgggc tgggcatcgg tgagacgttc gccggtgagc accacctgct tcttgaccag
    10921 cagggggctg ccgccgcgct cggagtacag ctcgctaccc ggcgggatgc tgccgttgag
    10981 ggccgcttcg agggcgcccg gggcgtcgtc caccatgcgg atctcgaggg tcgcggtgcg
    11041 gccgaggatg tccttggcct tggccacgtc ctgtacaccg ggcagctgca ccacgatgcg
    11101 gtcgccgccc tgctgctgga tcaccggttc ggcgaccccc agttcgttga tgcggttgtg
    11161 cagggtgacg atgttctggc ggatcgccga ttcctggatg ttcttctggg ccagcgggtt
    11221 gagggtggcc gtcagcttga aatcggcgcc gtcctggcct tcgctgagga gcaggtcatt
    11281 ggtgctggcg gtgatcaccg cgcgaccgcg ggcgagcgtc tcggcgtcgc ggaagcggat
    11341 cgccacactc tgaccttcgc ggctgatgcc ggcatggcgc acggacttgt cccgcagcag
    11401 ggtgcgcagg tcgccggcgg tggcgtcgag acgcttggtg acggcgccct tcatgtccac
    11461 ctgaagcagg aagtgcacgc cgccgcgcag atcgaggccc agatacatgg gcagggcatg
    11521 gatggcggtg agccactggg gactggcgga cagaaggttg agggcgacca cgtaggacgg
    11581 gtcggcgggg tccgggttga aggccttctc gatggcgtcc ttggccttga gctgggtgtc
    11641 ggtgtcgtgg aagcgggcgc ggacgctggt gagatcggcg aacagcccgt cgtgctggag
    11701 ccctgcgcca tggagggtgt cctcgacgcg cttgagggtg gcggtggcat ccagtttgag
    11761 ggtggccttg ccgctcgaga cctggacggc cggcacctct ccgtagaagt tcggcagggt
    11821 gtagagcaga ccccacagca gcacgaggcc gatgaggagg ttcttccaga ggggatagcg
    11881 attcatggtc gggcggaaag gcgtgaaacg gggcggagag cggtaccggc cggggcgtgc
    11941 cccggcggca ggcatcagag ggacttgagg gtgcccttgg gcaggacgat gccgatggcg
    12001 ggcttctgga cgacgacctg ggtgttggcg gcgatctcga gatgcacgta gttgtcgccc
    12061 acctgggcga ccttgcccac cagaccgccc tgggtgacga cctcatcgcc cttttgcagg
    12121 ccttcgatca gcgctttgtg ttccttggcg cgcttctgtt gcgggcggat cagcatgaac
    12181 cagaagatcg cgaacatcgc caccatgatc acgatctggc cgtagccggc ggtgggatcc
    12241 tgcgcggcag cgggggcggc ttgggcaaaa gcgttggaaa tcagcacatt aaactccggt
    12301 ggaacgtctc aaagtcgccg attatagcag cagcgggtga gcgcctcggg tggcttgaag
    12361 ggcgggacgt ggacaaaaag acggcgtccg gggaggacgc cgttgaatct ccacgaggga
    12421 gaggagggag aaaaacaccg tgaggctccg gcgcagcgcg tcaggcgcgc cggttgcggc
    12481 cgtttcaggc cgccttgcgc atctggttgc gatagcgctg ccagttggca gacatcgcgt
    12541 tgaacatgtc ttcggccgcc agcaggggca gcagccacat gcggtgctgg ctgcgcacaa
    12601 aggtttccat gtgcggctga cagagggtgg tctgctcgtc ctgcagcagt tcgaggaagc
    12661 gatccagggc agcgccccag aattcggagg tgccgaccca gccggtgtcg gcggtggctt
    12721 cgcggacggc cttgcgccac aaggcttcgg cccgttcgac ggagacgccg gccttgcggg
    12781 cgtaccaggg gaggatcttg ggagatttca tgctgttaac tcctgttggg atgcgaaaag
    12841 ggttccggtt gcacacattt ctggtctggt tttgttgcaa cgcaaatatt catttttatc
    12901 aaataaatca ttgcgttgtt tgtggcttcc gatctctgaa caccctttct gttgcattgc
    12961 acaatttcag tttaggcaag tccgccagac ggtgcaagct gccttgcatt ttgtttcacc
    13021 cccgtcgtga cggaactcgc atttttacaa cactgcccgt tcgggctgga tcgatgaatc
    13081 ggggcgtaga atgcgcgcct cccccgtatt cacgtcacca tgtcccagct ccttgaacac
    13141 ctcaacgcgg agcagctcgc tgccgtcacc ttgccaccgc gccatgccct gatcctggcc
    13201 ggcgccgggt cgggcaagac ccgggtgctg accacccgca ttgcctggct gatccagaac
    13261 ggccactgca gccctcaggg catcctcgcc gtgaccttca ccaacaaggc cgcgaaggag
    13321 atgctgacgc gcctctctgc gctgctgccc atcaacaccc gcggcatgtg gatcggcacc
    13381 ttccacggcc tgtgcaaccg cttcctgcgc gcgca
//
LOCUS       DAOQMM010000103        13379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1954_length_13379_cov_48.900931, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000103 DAOQMM010000000
VERSION     DAOQMM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1954_length_13379_cov_48.900931"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLH85_14185"
     CDS             <1..918
                     /locus_tag="PLH85_14185"
                     /inference="COORDINATES: protein motif:HMM:NF021201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2399 domain-containing protein"
                     /protein_id="HNB65762.1"
                     /translation="GERGLWRLRQLRFLAPAELRRALGLPEPDAERAAWAHARGHVFT
                     HPALAAAATVLDALPPARALARHALLRALDTWQADGRSGTWRDFSHFARGSTKLIGDS
                     ERAWLAAHLTLADFRLDDHTPLLLLRTAAELRLGDTHLTLAAIPDFVALSPDTLRQIT
                     GAASGPAVWFVVENRSSFERVARACGPDEGALWLPGYPPGSWRASVAGLLRHLPAPAR
                     IACDPDPDGILIACQAGTLWTAAGLDWTPWRMDAADFDALKTTRPLTERDRKLLDSLL
                     HDPTLPAPLTHLAKALATRGAKGEQEAYL"
     gene            1019..2119
                     /locus_tag="PLH85_14190"
     CDS             1019..2119
                     /locus_tag="PLH85_14190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB65763.1"
                     /translation="MPTSSAALPAPSQVLQLLAGAEALSRERDLRTLFRLGAEYAMRF
                     LDGRFGLFFQVRFTPPELTVGLLGCCVSDGADTHWDDVAEAQSWLPVDPLLAVCLHAD
                     HGHAIEQSGDIWRVAFAVGALGDARWALEIQTPRKPSKTDGEALSLFLRCFENVHRQW
                     EYANLDTLTRLLNRKTFDDDFDRLIAAAELAEQARQLPQDRRDPGLKITQPCWLAVVD
                     IDHFKHINDTYGHLFGDEVLLRLADLMRQSFRNHDKLFRFGGEEFVIMLRNVSEDHVQ
                     PIFDRFRMAVDRNDFPRVGHVACSIGFTRIDPRFSPAELLGRADEALYFSKEHGRNQV
                     NGFDTLIMSGQLRLSTPAGQAQIQSDVDEIFL"
     gene            2291..3796
                     /gene="lysS"
                     /locus_tag="PLH85_14195"
     CDS             2291..3796
                     /gene="lysS"
                     /locus_tag="PLH85_14195"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNB65764.1"
                     /translation="MSDQTSAPAQDENHLIAERRQKLAEWRATGKAFPNDFARENTAG
                     KLDEVYGEKSAEELEALPVEVKVAGRIMLKRVMGKASFATIQDLSGRIQLYVTRDGVG
                     EDVYAQFKHWDIGDIVGTVGTLFRTKTGELSVKSTELRLLTKSLRPLPDKFHGLADVE
                     TKYRQRYVDLIMSEESRRTFVARSRLVQSIRNYMTGHGFLEVETPMMHPIPGGAAAKP
                     FVTHHNALDMQQFLRIAPELYLKRLVVGGFEKVFEINRNFRNEGLSPRHNPEFTMMEF
                     YEAYADYRSLMDFTEGLLRHAAREALGTEVFQYQGRELDLSRPFHRLTIVEAIRKYHP
                     GFTEAQLADAEWIKTKIVAFGEKVKPGGLGSLQLQLFEACTEADLWEPTFIIDYPVEV
                     SPLARASDTDPEITERFELFIVGREIANGFSELNDPEDQANRFQAQALAKDAGDEEAM
                     YYDADYIRALEYGLPPTGGCGIGIDRLIMLLTDSPAIRDVILFPQMRPEAD"
     gene            complement(3896..6064)
                     /locus_tag="PLH85_14200"
     CDS             complement(3896..6064)
                     /locus_tag="PLH85_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65765.1"
                     /translation="MKAKSIQGRISMAAGSCLIGSAIALVAFSVISSDATKSVVAERV
                     STLVKNDTAESLKHLAGTQAGRIQAKFDLALDAARTMAHTFVLAKTPGSGVSLGRGQI
                     NAILKNVLENNPEFNGTYSCWEPNALDGQDAAHATGLNGDNAKTGRFTPYWNRGEGGR
                     IAVQPLVEYDTLDKHPNGVLKGGWYIGPRDQHTESVLDPFPYIVQGKQVWLTTLSVPI
                     MVDGKFYGVAGTDYNLNFVQQLAEETDKGLFEGRGEVTIVSNMGLVVADSEHPELIGK
                     RLELNGPANANLMTDIQSGKARGWTDEGSGIMSAVAPIALGRTGKPWAVLVRVPTKVV
                     LAQAQQLDDDIQARGRSSTILLIVAGLAISVGATAILWFAAGSIARPIREAAQFARLI
                     RRGDFSKRFQHHSEDEVGHLAHALDEMSQGLLSKARLAEQISEGDLNVDVELASDDDQ
                     LGRALKRMVDTLNNLVSELQGGANLIASGASQVADLSAVLTDGAGQSAESVTEIGAAI
                     SEMEVQTRNNAQNSGMANTISIRSRDAASLGSQHMDELTAAIQSISDASSRINGIINA
                     INEITNQTNLLALNAAIEAARAGETGRGFAVVADEVRKLASRSAEAAAQASQLISESG
                     ARTSHGIEIARRTADALKLIVTSASEVSELVAQIATASQDQASGISEVSAGLGQIDGV
                     AQRTNGHAVECSEAAAALTKQADHVKHLMGRFRVRSGRPV"
     gene            6599..7138
                     /locus_tag="PLH85_14205"
     CDS             6599..7138
                     /locus_tag="PLH85_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB65766.1"
                     /translation="MSVRDRLLDAGVSLLRDQGASALTQPRIAAAAGVSQSHLTYYFP
                     TRDALLLAIAEHSVDQELQRATADSGGHPAERLAQAARFLPRVRMMLGLVASGDRDPA
                     IRPAFARLATHVREALGQLLAHHGYTVSAPQVVVVHAALVGLAIMNLGRQDATSDADV
                     ELGLRQLLALLPHPERTEP"
     gene            7135..8103
                     /locus_tag="PLH85_14210"
     CDS             7135..8103
                     /locus_tag="PLH85_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB65767.1"
                     /translation="MTSRACLPIALSLAVAACGTPERSDFQGYVEGEYLYLAAPQAGY
                     LKHLNAPRGRRVADGEPLFEIAADPDDQALAEAQARAGAAREKLENLREPRRTPEIAA
                     LEAALTGAVAARRLAEVQLQRQQALSRQQFVSPAAVDDARARRDQAVAGEDAARQQLA
                     TARIALGRAPEVRGAEADLRAADAQAAQKRWALERKTVAAPTAGEISDTYYRPGEWIP
                     AGAPVTSLLPDTRRRLRFYVPETALAGLQPGQVVEARCDGCAAPIRATIDFIAPQAEY
                     TPPVIYSRGTREKLVFRVEAAPAPDDARRLRPGLPVDVRLAERRDG"
     gene            8096..9022
                     /locus_tag="PLH85_14215"
     CDS             8096..9022
                     /locus_tag="PLH85_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB65768.1"
                     /translation="MADWVIDVCGLNKHFGARHVVRDLSLQVRAGEIFGFLGPNGSGK
                     TTSIRMLCGLLTPDSGAGTCLGHDVIRESAAIKRQVGYMTQKFSLWDDLSLAENLDFV
                     ARMFGMDDRRAAVDRTLVELGLEGRRHQLAGTLSGGWKQRLALAACLLHRPRLLLLDE
                     PTAGVDPKARRDFWEEIHRLSAAGITILVSTHYMDEAERCHRLAYIAYGNLLASGTAA
                     EVVAGRGLHTWAVSGAASHGLADTLRRLPGAEQVASFGTALHVSGTDGARLGTALDEL
                     GRRGDLRIEAVQPSLEDAFIHLMQHSQDPLAG"
     gene            9028..10152
                     /locus_tag="PLH85_14220"
     CDS             9028..10152
                     /locus_tag="PLH85_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB65769.1"
                     /translation="MGIPDRFSFSRWLGILIKEFIQLKRDRLTFGMIVGIPILQLLLF
                     GFAINSDPRHLPTAVVMADPGPFARAYVAALRNSDYFDVRGSVDAREADRLLERGDVL
                     FVVSFPPGFHRDLVRGRSPTVLVEADATDPMATAGAISVLNKLGAEVFAADLPGLERP
                     PAAAIDLRVQRRYNPEGITAYNIVPGLMGVILTMTMVLMTGLAMTRERERGTFENLLA
                     TPATPLEVMTGKIVPYILIGLIQVSLILAAARWIFDVPMFGNLLVLYAVVLLFICANL
                     TLGITFSSLARNQLQAMQMTFFFFLPSMLLSGFMFPFRGMPEWAQAVGNLLPLTHFLI
                     LVRGILLKGNGVLWEQVWPILAFMGAVLAVGLKTFRRTLD"
     gene            complement(10184..11785)
                     /locus_tag="PLH85_14225"
     CDS             complement(10184..11785)
                     /locus_tag="PLH85_14225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB65770.1"
                     /translation="MKKSVLPRVGPVARLSLGLVALTLTLLMAGDWLFGFASSETAMI
                     QEARRREGENLAAQLTVLVEANDELGLGRTLQRILTRNGELRSLAVRRSDGTLMAQRG
                     DHAVHWIAPESGRSTLEHLRVPLNAGRSHWGDVEISFAPTHAPGAGAWLAQPLTRIIL
                     LVSVGGFLVYYAYLRRAMQFLDPSSAVPERVRKAFDTLTDALLVLDADGRIVLANKAF
                     RQLHGESPTRLIGRRIADLDWLDAGMESFEKGRAPWERVLRGDDLVTDVRMSVVRPGG
                     GTLELAVSCSPVRDEYGNTRGCLVSFDDLSELERANQDLRKALSEIEQSRARIKAQNE
                     KLTHLASRDPLTGALNRRAFFEKVAGPFETALRGNGQLCCLMTDIDHFKRFNDIYGHA
                     VGDQVIKVVVRCIEKHIREADMLCRYGGEEFCVVMPNADLEAGMAIAERIRSMVASTA
                     GSMIRNTDVGTITSSFGIASITDGAATIEDLIEQADTALYASKKGGRNRTTAWSPGSP
                     SMEEMDAVAAAAAAEPPRESAPAAS"
     gene            complement(11782..>13379)
                     /locus_tag="PLH85_14230"
     CDS             complement(11782..>13379)
                     /locus_tag="PLH85_14230"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNB65771.1"
                     /translation="DNVYDLIVQVSDGQRTGTQTLAIHVINVNEAPVATADAFAGNED
                     AVLTGNVLGNDADVDGDALSARWVSGPAHGTLVLGADGRFTYTPDANFNGADSFSYVA
                     HDGALDSAPVTVSLHLAPVNDPPVSGVVTLAPHPEDGGPRLITQAELLSAANDVDGDP
                     LVARDLAIASGRGTLSDNGDGTWTFVPASNDDTRVGFSFVISDGHAETPATAQLSLIP
                     VNDAPAAADAAFATQDTQRHVGQLPAASDVEGDPVVYGLVTAPIRGTVVVQPDGRFTY
                     TPTPGNGGSDAFSFAVTDVHGASTTHTVTVVITVAGGGSGAGTGTEGDTSSDGATSTG
                     RKTRDTTPGVVKTTAPRTATQAESGAAAQVSRIVTAALRGPQEAPASDAVVAPASSGS
                     PPAMHHLRRGANGADLADWENIRFSWFSSYSMDFRHSRGSAVQDWTPATGHPGGREEG
                     EALLETLPAKVAGVSLSVGVVWWAARAGGLLASLAASLPAWRGVDMLMVLKDREPDDD
                     EAGDEDHPGGLPGADDRDERSDG"
BASE COUNT         2112 a         5025 c         4061 g         2181 t
ORIGIN      
        1 ggcgagcgcg gcctgtggcg cctccgccag ctccgcttcc tcgcccccgc cgaactgcgc
       61 cgcgccctcg gcctgcccga gccggacgcc gagcgggccg cctgggcgca cgcccgcggg
      121 cacgtcttca cccatcccgc gctggccgcc gcagccaccg tcctcgacgc gctgccgccc
      181 gcccgcgccc tcgcccgcca cgccctgctg cgcgccctcg acacctggca ggccgatggc
      241 cgcagcggca cctggcgcga cttctcccac ttcgcccggg gcagcaccaa actgatcggt
      301 gactccgaac gcgcctggct cgctgcccac ctcaccctgg ccgacttccg cctggacgac
      361 cacacgcccc tcctcctgct gcgcacggcg gccgaactcc gcctcggcga cacccacctg
      421 accctcgctg cgatcccgga cttcgtcgcc ctgtcgcctg acaccctgcg ccaaatcacg
      481 ggcgccgcgt ccggccccgc cgtgtggttc gtggtggaga accgcagcag cttcgagcgg
      541 gtcgcccgcg cctgcgggcc cgacgagggc gccctgtggc tgcccggcta cccgccaggc
      601 agctggcgcg cgtccgtcgc aggactgctc cgccatctcc ccgctcccgc ccgcatcgcc
      661 tgcgatcccg acccggatgg cattctcatt gcctgccagg ctggtacgct gtggacggcc
      721 gccgggctcg actggacgcc ctggcgcatg gacgcggcag acttcgacgc cctcaaaacc
      781 acgcgccccc tcacggaacg cgaccgcaag ctgctggaca gcctgcttca cgacccgacg
      841 ctgcctgccc ccctcaccca cctcgccaag gccctcgcca cccgcggcgc caaaggcgaa
      901 caggaggcct acctgtaagc ccctgatgcg cctcacaaag ggaattgcgc aagacgcttc
      961 agaatctcgc tgacacgacg ttattccgcc tcacgaaccc acgcaccctg cgtcgtccat
     1021 gcccacgtcc tctgcggcgt tacccgcccc ctcccaggtg ctccagctgc tcgccggagc
     1081 cgaggccctg tcccgcgagc gggacctgcg gaccctgttc cgcctggggg ccgagtacgc
     1141 catgcgcttc ctggacggac gcttcggcct gttcttccag gtccgcttca cgccaccgga
     1201 actcaccgtc ggtctgctcg gctgctgtgt ctcggatggc gccgataccc actgggacga
     1261 tgtggccgag gcccagtcct ggctgccagt cgatccgctg ctggcagtct gcctgcatgc
     1321 cgaccatggc catgccatcg agcagtccgg cgacatctgg cgcgtcgcct ttgccgtcgg
     1381 cgcccttggc gacgcccgct gggcccttga gatccagacg ccgcgcaagc cgtcaaaaac
     1441 cgatggcgaa gccctctccc tgttcctgcg ctgcttcgag aacgtccatc gccagtggga
     1501 atacgccaac ctggacaccc tcacgcgcct gctcaaccgc aagaccttcg acgacgactt
     1561 cgaccgcctc atcgccgctg cggaactcgc cgagcaggcc cgccagctgc ctcaggaccg
     1621 gcgggatccg ggcctcaaga tcacccagcc gtgctggctc gccgtggtgg acatcgatca
     1681 tttcaagcac atcaacgaca cctacggcca tctcttcggt gacgaggtcc tgctgcgcct
     1741 cgccgacctc atgcgccaga gcttccgcaa ccacgacaag ctgttccgtt tcggcggcga
     1801 agagttcgtg atcatgctgc gcaacgtctc ggaggaccac gtccagccca tcttcgaccg
     1861 cttccggatg gccgtggatc gcaacgattt tccccgcgtc ggccatgtgg cctgctccat
     1921 cggcttcacc cgcatcgacc cgcgcttctc gcccgccgag ctcctcggcc gcgccgacga
     1981 ggccttgtac ttctccaagg aacacggccg caatcaggtc aatggcttcg acaccctgat
     2041 catgagcggc cagctgcgcc tgtccacccc ggccggccag gcccagatcc agtccgacgt
     2101 ggacgagatc ttcctgtagc ggccgcattc ccgcctgccc acccggccgc acgcaggtcc
     2161 ttccggccca ccgcgccgcg gccggccgcg acgtgcgccc ccggatgtgt caaaattcag
     2221 ggttttacgg cattcccggc gcaccgtctg cgccccgccg accccgattc cgttgattga
     2281 agagcccatc atgtccgacc agacctccgc gcccgcccag gacgaaaacc acctgatcgc
     2341 cgaacgccgc cagaagctcg ccgaatggcg cgccaccggc aaggccttcc cgaacgattt
     2401 cgcccgggag aacaccgccg gcaaactcga cgaagtctat ggcgagaaga gcgccgagga
     2461 gcttgaggcc ctgcctgtcg aggtcaaggt cgccgggcgc atcatgctca agcgggtgat
     2521 gggcaaggcc agcttcgcca cgatccagga tctctccggc cgcatccagc tctatgtcac
     2581 ccgcgacggc gtgggcgagg acgtctacgc ccagttcaag cactgggaca tcggcgacat
     2641 cgtcggcacc gtgggcaccc tgttccgcac caagaccggc gagctgtcgg tcaagagcac
     2701 cgagctgcgc ctgctgacca agagcctgcg ccccctgccg gacaagttcc acggcctggc
     2761 cgacgtggag accaagtacc gccagcgcta cgtggacctc atcatgagcg aggagtcccg
     2821 gcgcaccttc gtggcccgca gccgcctcgt ccagtccatc cgcaactaca tgaccggcca
     2881 cggcttcctc gaagtcgaaa cgcccatgat gcaccccatc cccggcggcg ccgcggccaa
     2941 gcccttcgtc acccaccaca acgccctcga catgcagcag ttcctgcgca tcgcgccgga
     3001 gctctacctc aagcgcctgg tggtgggcgg cttcgagaag gtcttcgaga tcaaccgcaa
     3061 cttccgcaac gaaggcctct cgccccgtca caacccggaa ttcacgatga tggagttcta
     3121 cgaggcctac gcggactacc gcagcctcat ggatttcacc gagggcctgc tgcgccacgc
     3181 cgcacgggaa gccctcggca ccgaggtgtt ccagtatcag ggccgcgaac tggatctctc
     3241 caggcccttc caccgcctca ccatcgtcga agccatccgc aagtaccacc ccggctttac
     3301 cgaggcccag ctcgccgacg cggagtggat caagaccaag atcgtggcct ttggcgagaa
     3361 ggtcaagccg ggcggcctgg gcagcctgca gctccagctc ttcgaagcct gcaccgaagc
     3421 cgatctgtgg gaacccacct tcatcatcga ctacccggtc gaagtgagcc cgctggcccg
     3481 cgcctccgac accgacccgg agatcaccga gcgcttcgaa ctcttcatcg tcggccgcga
     3541 gatcgccaac ggcttttccg aactcaacga cccggaagat caggccaacc gcttccaggc
     3601 ccaggccctg gccaaggacg ccggtgacga ggaggccatg tactacgacg ccgactacat
     3661 ccgcgccctc gaatacggcc tgcctcccac cggtggctgc ggcatcggca tcgaccgcct
     3721 gatcatgctg ctcaccgaca gcccggcgat ccgcgacgtc atcctcttcc cgcagatgcg
     3781 ccccgaagcg gactgacacg cagccccgcg cagccccctt gcgaggcccg cccggcgtct
     3841 gcccggcggg cctcgccgca tccgcgccag gggcaggaca gggccacgcc ggacatcaga
     3901 caggccgccc gctgcgaacc cggaagcgcc ccatcaggtg cttgacatgg tccgcctgtt
     3961 tggtcagggc cgccgcggcc tcactgcatt ccacggcgtg gccgttggtt cgctgggcga
     4021 ctccgtcgat ctgccccagc cccgcgctca cttcactgat gccggatgcc tgatcctggg
     4081 acgccgtggc aatctgggcg accagttcgg aaacctcgct cgcgctggtg acgatgagct
     4141 tcagcgcatc ggccgtgcgc cgggcgatct cgatgccatg ggacgtccgg gcgcccgatt
     4201 cgctgatgag ctgggaggcc tgggccgcag cctccgcact gcgcgacgcc agcttgcgca
     4261 cctcgtccgc cacgacggcg aagccccggc cggtctcgcc ggcccgcgcc gcctcgatgg
     4321 ccgcattcag cgcgagcaga ttggtctgat tggtgatttc attgatggca ttgatgatgc
     4381 cgttgatgcg gctgctggca tcgctgatgg actggatggc cgcagtcagc tcgtccatgt
     4441 gctggctgcc caggctggcg gcgtcccgtg agcggatgga gatcgtgttg gccatgcccg
     4501 agttctgggc gttattgcgg gtctgcactt ccatctcgga aatcgccgcc ccgatctcgg
     4561 tgacggactc ggccgactgg ccggcaccgt cggtcaggac cgcactcagg tcggccacct
     4621 gactggcgcc tgaggcgatc aggttggcgc cgccctgcag ttcggaaacc agattgttca
     4681 aggtatccac catccgtttc aaggcgcgtc cgagctgatc gtcgtcggac gcgagctcga
     4741 cgtccacatt gaggtcgcct tcgctgatct gctcggcgag cctggccttg ctcagcagcc
     4801 cctgggacat ctcgtcgagg gcgtgggcca ggtgccccac ttcgtcctcg gaatggtgct
     4861 gaaagcgctt cgagaagtcg ccgcgacgga tcagccgggc gaactgggcc gcttcgcgga
     4921 tggggcgggc gatggaaccg gccgcaaacc acaggatggc cgtggcgccg acgctgatgg
     4981 ccagcccggc aacgatcagc aggatcgtgg agctccgtcc acgcgcctgg atgtcgtcgt
     5041 cgagctgctg cgcctgggcc agcacgacct tcgtcggcac ccgcaccagt accgcccacg
     5101 gcttgccggt gcggcccagg gcaatcggtg ccaccgcgct catgatcccc gagccctcat
     5161 cggtccagcc gcgggccttg ccgctctgga tgtcggtcat caggttggcg ttggcaggtc
     5221 cgttgagctc gagacgtttg ccgatgagct cgggatgctc actgtcggcc accaccagcc
     5281 ccatgttgct gacaatggtg acctcgccgc ggccctcgaa cagccccttg tcggtttcct
     5341 cggcaagctg ctggacgaaa ttgagattgt agtcagtccc ggcaacccca taaaacttgc
     5401 cgtccaccat gatcggcacc gaaagcgtgg tgagccacac ctgctttccc tgcacgatat
     5461 acgggaacgg gtccagcacg ctttccgtat gttgatcacg ggggccgata taccatccgc
     5521 ctttcaatac accgttggga tgtttgtcca gggtgtcgta ttcaaccaga ggctgcaccg
     5581 cgatgcgccc cccctcgcca cgattccaat agggcgtgaa gcgccccgtc ttggcattgt
     5641 cgccgttcaa tccggtggca tgagcggcgt cctgtccgtc gagggcgttg ggctcccagc
     5701 atgaatacgt cccgttgaac tccgggttgt tctccagcac gttcttgagg attgcattga
     5761 tctgtccccg ccccaggctg accccgctgc ccggagtctt ggcgagaacg aaggtatggg
     5821 ccatggtccg cgccgcgtcc agcgccaggt cgaacttggc ctgaatgcgc cccgcctgcg
     5881 tgcctgcgag gtgtttgagg ctctcggcgg tatcgttctt gaccagcgtg ctgacccgct
     5941 ctgcaacgac actcttcgtg gcatcggacg agatgacgct gaaggcgacc agggcaatcg
     6001 cggaaccgat cagacaactc ccggcagcca tggaaatacg cccttgaatg gatttcgctt
     6061 tcatttttgg tcttcttgct aattgaatag ttgtccagga cgaatcaaca acacaggcaa
     6121 ggccgccatg cattcatgcg ccggggccaa attcatatcg gcgacacatt aaggcgcttt
     6181 agactcataa ataaaatttt tatcgccaac ccccatcccg gcaagggctg tccatttatt
     6241 aaacaaactc cttttacgtt cgcgtcaatt acagagcgca attcgacaaa caattgtcca
     6301 ttaattaaac aactacccat ttgattgatc cggatcaatt catgaccatc aattctctga
     6361 ccacggaatt cggattcgca aggaatcggc aatcggtctc tgcgcccggt gggaccgtgc
     6421 gcttcgcgcg ccctcctgcg ccagcgcccg aagagaatcg ggggagactg cccgacggac
     6481 cggtctccgc agacatgcca cctgcgtcca tggaccgcca gccgcatccc acgctcgaca
     6541 tttggtagca tcaccaggat cttggtagaa taaccaaacc cttgcagacc tccgcgccat
     6601 gtccgtccgt gaccgcctcc tcgatgccgg cgtgagcctc ctccgcgatc aaggcgcctc
     6661 tgccctgacc cagccgcgca ttgccgcggc cgccggggtc agccagagcc acctcaccta
     6721 ctacttcccc acccgcgacg ccctcctgct ggccatcgca gagcattcgg tcgatcagga
     6781 actccagcgc gccaccgccg acagcggcgg ccatccggcc gaacgcctgg cccaggccgc
     6841 gcgtttcctg ccccgggtgc gcatgatgct gggcctggtc gcctcgggag accgggaccc
     6901 ggccatccgc cccgcctttg cacgcctcgc cacccacgtg cgcgaggccc tcggccagct
     6961 cctcgcccat cacggctaca ccgtcagcgc accccaggtg gtggtggtcc atgccgccct
     7021 cgtggggctg gccatcatga acctcggccg ccaggacgcc acctccgacg ccgacgtgga
     7081 gcttggcctg cgccaactcc tcgccctcct gccccatccg gaaaggaccg aaccatgacc
     7141 tcgcgcgcgt gcctcccgat cgccttgagc ctggccgtgg ccgcctgcgg cacgccggag
     7201 cggagcgact tccagggcta cgtggaaggc gaatacctct atctcgcggc gccccaggcc
     7261 ggctatctca agcacctgaa tgccccgcgt ggccgtcgcg tcgccgatgg cgagccgctc
     7321 ttcgagatcg ccgccgaccc ggacgaccag gccctcgccg aagcccaggc ccgcgccggc
     7381 gcggcccgcg aaaagctcga gaacctgcgc gaaccccgcc gcacgcccga gatcgccgcc
     7441 ctcgaagccg cgctcaccgg cgccgtcgcc gcacgccgtc tggccgaggt ccagctgcag
     7501 cgccagcagg ccttgtcgcg ccaacagttc gtctcgccgg ccgccgtaga cgacgcccgc
     7561 gcccgccgtg atcaggccgt cgccggtgaa gacgccgccc gccagcaact cgccaccgcc
     7621 cgcatcgccc tcggccgcgc gccggaagtg cgcggcgccg aagccgatct gcgcgccgcc
     7681 gacgcccagg ctgcccagaa acgttgggcc ctcgagcgca agaccgtcgc cgcgcccacc
     7741 gcaggcgaga tcagcgacac ctactatcgc cccggcgaat ggattcccgc cggcgcgcca
     7801 gtgaccagcc tgctaccgga cacgcgacgg cgcctgcgct tttacgtacc cgaaacggcc
     7861 cttgccggcc tgcaaccggg ccaggtcgtg gaagcccgct gcgacggttg cgcggcgccg
     7921 atccgtgcca ccatcgactt catcgccccc caggccgaat acaccccgcc ggtcatctac
     7981 agccgcggca cccgcgagaa gctggtgttt cgcgtggaag ccgcgccagc gcccgacgac
     8041 gcgcgccgcc tgcggcccgg tctgccggtg gacgtgcgcc tcgcggagcg ccgcgatggc
     8101 tgactgggtc atcgacgtgt gcggactcaa caagcacttt ggcgcccgtc atgtggtgcg
     8161 cgacctcagc ctgcaggtcc gcgccggcga gatcttcggc ttcctcggcc ccaacggcag
     8221 cggcaagacc acctccatcc gcatgctgtg cggcctgctc acgccggaca gcggcgccgg
     8281 cacctgcctg ggccacgacg tgatccgcga gagcgccgcc atcaagcgcc aggtgggcta
     8341 catgacccag aagttctccc tgtgggacga cctcagtctg gccgagaacc tggacttcgt
     8401 cgcccgcatg ttcggcatgg acgaccgccg cgccgcagtg gaccgcaccc tcgtggagtt
     8461 gggcctggag ggccgccgcc accagctggc gggcaccctc tcgggcggct ggaagcagcg
     8521 cctcgccctc gccgcctgcc tgctccaccg acccaggctg ctcctcctgg acgaacccac
     8581 cgcgggcgta gaccccaagg cccggcgcga cttctgggag gagatccacc gcctctccgc
     8641 cgccggcatc accatcctgg tgtccaccca ctacatggac gaggccgagc gctgccaccg
     8701 cctcgcctac atcgcctacg gcaacctgct ggcttccggc accgccgccg aggtggtggc
     8761 cggccgcggc ctgcacacct gggcggtcag tggagccgcc agccacggcc tggccgacac
     8821 cctgcggcgc ctgcccggcg ccgagcaggt ggccagcttc ggcaccgccc tgcacgtgag
     8881 cggcaccgac ggtgcgcgcc tcggcaccgc cctggatgag ctgggccgcc gcggcgatct
     8941 gcgcatcgag gcggtccagc cctccctgga ggacgccttc atccacctca tgcagcacag
     9001 ccaggacccc ctcgcgggct gacggccatg ggcattccgg accgcttctc cttctcccgc
     9061 tggctgggca tcctcatcaa ggagttcatc cagctcaagc gcgaccgcct caccttcggc
     9121 atgatcgtcg gcatcccgat cctgcaactg ctgctcttcg gcttcgccat caactcagat
     9181 cccaggcacc tgcccaccgc agtggtgatg gccgacccgg gcccctttgc ccgcgcctac
     9241 gtggcggccc tgcgcaacag cgactatttc gacgtgcgcg gcagcgtcga cgcacgcgag
     9301 gccgaccgcc tgctggagcg gggcgacgtg ctcttcgtgg tgagctttcc gccgggcttc
     9361 catcgcgacc tggtacgcgg ccgcagcccc acggtcctgg tcgaggccga cgccaccgac
     9421 ccgatggcca ccgccggcgc catctcggtg ctcaacaagc tcggcgcgga ggtcttcgcg
     9481 gcggatctgc cggggctgga gcgcccgccc gccgcggcca tcgacctgcg cgtgcagcgc
     9541 cgctacaacc cggaaggcat caccgcctac aacatcgtgc ccggcctgat gggcgtgatc
     9601 ctgaccatga ccatggtgct catgaccggc ctggccatga cccgcgagcg ggagcgcggc
     9661 accttcgaaa acctcctcgc cacgccggcc acgccgctgg aggtgatgac cggcaagatc
     9721 gtgccctaca tcctcatcgg cctgatccag gtcagcctga tcctcgccgc cgcccgctgg
     9781 atcttcgacg tgcccatgtt cggcaacctg ctggtgctct acgcggtggt gctgctcttc
     9841 atctgcgcca acctgaccct gggcatcacc ttcagctccc ttgcccgcaa ccagctgcag
     9901 gcgatgcaga tgactttctt ctttttcctg ccctccatgc tcctgtccgg cttcatgttt
     9961 cccttccggg gcatgcccga gtgggcgcag gcagtgggca acctgctgcc cctcacccat
    10021 ttcctgatcc tggtgcgggg catcctgctc aaggggaatg gcgtgctgtg ggaacaggtg
    10081 tggcccatcc tcgccttcat gggtgccgtg ctggcggtgg gcctcaagac cttccgccgg
    10141 acgctggact gacgcgcacg cgccgggcgt caggcgggcg acatcacgac gcggccggcg
    10201 cggattcccg cgggggctcc gcagcggccg ccgctgccac ggcgtccatc tcctccatgc
    10261 tgggcgaacc cggcgaccag gccgtggtgc ggttgcggcc acccttcttg gaggcataca
    10321 gcgcggtgtc ggcctgctcg atcaggtcct cgatggtcgc cgcaccgtcg gtgatggagg
    10381 cgatgccgaa gctggacgtg atcgtgccca cgtcggtgtt gcggatcatc gatccggcgg
    10441 tgctcgccac catgctgcgg atgcgctcgg caatggccat gcccgcctcc aggtcggcgt
    10501 tgggcatcac cacgcagaac tcctcgcccc cgtagcggca cagcatgtcg gcctcgcgga
    10561 tgtgcttttc gatgcagcgc acgaccacct tgatgacctg gtcccccacc gcgtgaccgt
    10621 agatgtcgtt gaagcgcttg aagtggtcga tgtcggtcat caggcagcac aactggccat
    10681 tgccgcgcag ggccgtctcg aagggcccgg ccaccttctc gaagaaggcg cgccggttga
    10741 gggcgccggt cagcggatcg cgggacgcca ggtgggtcag cttctcgttc tgggccttga
    10801 tgcgggcgcg cgattgctcg atctccgaca gggccttgcg caggtcctgg ttggcccgct
    10861 ccagctccga cagatcgtcg aagctcacca ggcagccgcg ggtgttgccg tattcgtcgc
    10921 gcaccggcga gcaactcacc gccagttcca gcgtcccgcc gccgggacgc accacgctca
    10981 tgcgcacgtc cgtgaccaga tcgtcgccac gcagcacccg ctcccacggg gcgcgcccct
    11041 tctcgaagga ctccatgccc gcgtccagcc agtccagatc ggcgatgcgc cggccgatca
    11101 ggcgggtcgg cgactcgccg tggagctgcc ggaaggcctt gttggcgagc acgatgcgtc
    11161 cgtccgcgtc caggaccagc agcgcgtccg tgagcgtgtc aaaggccttg cggacccgct
    11221 ccggcactgc gctggacgga tcgaggaact gcatcgcccg ccgcaggtag gcgtaataca
    11281 cgagaaaccc gcccacgctc accagcagga tgatccgcgt cagcggctgg gccagccacg
    11341 ccccggcgcc gggcgcgtgg gtcggcgcaa aactgatctc cacatcgccc caatggctgc
    11401 gcccggcatt gagcggtacc cggagatgct ccagcgtgga ccggccagac tccggcgcga
    11461 tccagtgcac ggcatggtcc ccccgctggg ccatcagcgt gccgtcggaa cgccggacag
    11521 ccagcgagcg cagctcaccg ttccgcgtca gtatcctttg cagcgtccgg ccaagcccga
    11581 gttcgtcgtt cgcctccacc agcaccgtca gttgggccgc cagattctca ccctcacgcc
    11641 gacgggcctc ctggatcatt gcggtctcgc tggatgcgaa cccgaacagc cagtcccccg
    11701 ccatcagcag cgtcagggta agcgccacca gccccagact gaggcgggcc accggtccca
    11761 cgcgcggcaa tacgctcttc ttcatccgtc gcttctttcg tcccgatcat ccgctcccgg
    11821 cagaccgccg gggtgatcct cgtcccccgc ttcatcgtcg tccggctccc ggtccttgag
    11881 caccatcagc atgtccacgc cgcgccaggc aggcagcgaa gccgcgagac tggcgagcag
    11941 accgcccgcg cgggctgccc accacaccac cccgaccgac agggacacgc cggccacctt
    12001 ggcaggtaag gtttcaagga gtgcctcgcc ctcctcccgg ccgcccggat ggccggtggc
    12061 gggcgtccag tcctgcaccg ccgaaccgcg gctgtgccgg aagtccatgg aatagctgga
    12121 gaaccaggag aagcggatgt tctcccagtc cgccagatca gcaccgttcg ccccccggcg
    12181 aaggtggtgc atcgccgggg ggcttcccga cgatgccggc gccacgacag catcgctggc
    12241 aggcgcctcc tgcggccccc gcagggccgc cgtgacgatc cggctgacct gggcggcggc
    12301 gcccgattcc gcctgcgtcg ccgtgcgggg cgccgtggtc ttgacgacac cgggcgtcgt
    12361 atcgcgggtc ttccggccgg tgctggtcgc gccgtcggaa ctggtgtccc cttccgttcc
    12421 ggtgccggcc cccgacccgc cgccggcgac cgtgatcacc actgtcaccg tatgggtggt
    12481 cgaagcgccg tggacgtctg tcacggcaaa actgaacgca tccgacccgc cgttgccggg
    12541 cgtcggcgtg taggtgaacc gcccgtcagg ctgcaccacg accgttcccc ggatcggcgc
    12601 cgtgaccaac ccgtagacga cgggatcgcc ttccacatcg ctggcggccg gcaactggcc
    12661 gacatggcgc tgggtatcct gggtcgcaaa ggcggcatcc gcggcagccg gcgcatcatt
    12721 gacggggatc aggctcaact gcgcggtcgc cggcgtctct gcatgtccgt ccgaaatcac
    12781 gaaactgaaa ccgacccggg tatcgtcatt ggacgccggc acgaaggtcc aggtgccatc
    12841 cccgttgtcg ctgagcgtgc cccgcccgct ggcaatggcg agatcgcgcg ccaccagggg
    12901 gtccccatcc acgtcgtttg ccgcggacag aagctccgcc tgcgtgatga gacgcggccc
    12961 gccatcctcg ggatggggcg ccagggtcac cacccccgat accggcggat cgttgaccgg
    13021 cgccaggtgc agactcacag tcacgggggc actgtccagc gcgccgtcat gggccacata
    13081 gctgaagctg tcggcgccgt tgaagttggc gtcaggcgtg taggtgaaac gcccatctgc
    13141 ccccagcacc agcgtcccat gggccggccc ggacacccac cgcgccgaca gtgcgtcgcc
    13201 atccacatcg gcgtcattgc ccaggacgtt gccggtcagc accgcgtcct cattcccggc
    13261 aaaggcatcg gcggtcgcaa cgggtgcttc attgacgttg atgacatgaa tcgcgagcgt
    13321 ctgcgttccg gtcctttggc cgtcgctgac ctggacgatc aggtcgtaga cgttgtcgg
//
LOCUS       DAOQMM010000104        13311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_1965_length_13311_cov_53.317592, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000104 DAOQMM010000000
VERSION     DAOQMM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1965_length_13311_cov_53.317592"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..322
                     /locus_tag="PLH85_14235"
     CDS             140..322
                     /locus_tag="PLH85_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB65772.1"
                     /translation="MNVTKPRRTRRTHSEEFKRSLIEACGEPGASVAGVALANGINAN
                     QLRRWMRERGIDPPAL"
     gene            complement(530..2047)
                     /locus_tag="PLH85_14240"
     CDS             complement(530..2047)
                     /locus_tag="PLH85_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65773.1"
                     /translation="MKRFESVGLLHIAALLATVLTAYLPQLYEFVGTDIGVYYEIGRR
                     LATDGRLELWGNYWDHKPPLIYLYFAIFSHLDALLGDTSGLKLGALCAYSGLTLLAYR
                     ALSIARPTDNRTLAALGSAVATSLVLGWLDPVANGILMVSALICAFSGLVLCADGKRD
                     TTVFLGGALIGLAPFLRPTALTALPFMLTLLFLEYRKTGERRRLWLALSGAVMVAGGC
                     ALWLLTFGGSAVFDSLIHYNADYGSRARSTTAVADFFFQDPIALCLAGVLLTSGVASL
                     SCATSPHRSTSLVLWAWALTEVASIVFQRKIQSFYLFGFILPLLLAFTYSALNATQTF
                     RTIAAVLLSTILAGTLGYSAHKLDLRILQNPLVSQHTQKTNAIAQIMSAQNCKGVPND
                     VLVYGNRAQIYTHAAAVGIRPYSWTVFLNPVVDGSLFADRQRQLVDSLTANPASFIVY
                     VGGDGWSPNIGGRIEKIEALIKQRYTAIPVAQTGGLETPYNYQYRLYRKAECAEQ"
     gene            complement(2044..3393)
                     /locus_tag="PLH85_14245"
     CDS             complement(2044..3393)
                     /locus_tag="PLH85_14245"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB65774.1"
                     /translation="MNTAPIAIIGAGFTGLSIAYRLVQAGVPVTVLEAEPDIGGLAAA
                     FPVGGQTLDRFYHHWFTNDREVMTLIEELGLSDKVTVNPTNTGIYYAHQFFRLSTPLD
                     LLCFKPLGLLARLRLGLLALRARRVRDWHSLESLTAHEWLRALGGEDVYRVVWQPLLQ
                     GKFGPYADEVSAVWFWNKLKLRGGSRGKAGEERLAYFEGGFVALAEALARHIVAAGGR
                     IVRNARVTGVRPDGNQWQLETTAGQFRAHEVAATVALPLIADMIDTWAPADYLAGLRR
                     IRYLGNVCMVLELDRSLSDTYWLNVNDPSFPFVGIIEHTNFARPETYGGRHIVYLSKY
                     LPVTDTLFGLDADSFLAYALPYIQTMFPAFRREWVVAHHLWRARWSQPVVERFYSRLI
                     PPTQGPRQGFHVCSMAQIYPEDRGTNYALREGRRLAEAIIACRRTTSHSASFASEVP"
     gene            complement(3390..4376)
                     /locus_tag="PLH85_14250"
     CDS             complement(3390..4376)
                     /locus_tag="PLH85_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB65775.1"
                     /translation="MRRTSRTLLGLGLAGLFLWLILRQVDSDEMLRILGGARPDWTAL
                     AIAAFFCGYACRIERWRLLLCHDNPTLRWRHCAGPLLASVAANNVLPFRLGDLLRLFG
                     FNARLGIGTTTAFASLFQERLLDLLVLLIILGVLPVALGLDISRLMGAGTAVFLAGAS
                     LILGLLLRPTAIHPPVKIIVSFVTRWHASLGQRLDRESARVFTLLTQSAQPRLALRLL
                     MWSMLAWLIEGLVFYCCALALPAITQPAAAWLALPLGTLATLLPSSPGYLGTFDYFTA
                     WALSSAGNTATASATYAFFVHAILWGPVTLAGGCYVIARPVGVSARRQGNAP"
     gene            complement(4379..5371)
                     /locus_tag="PLH85_14255"
     CDS             complement(4379..5371)
                     /locus_tag="PLH85_14255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB65776.1"
                     /translation="MDTSDTPLLSIVIPCFNEEDVITSTVNRLRAFCATVKDVRVELI
                     FVDDGSQDDTRARLKSFGTEDPRIRIIGFARNFGHQVAVTAGIDAARGDAVVLIDADL
                     QDPPEVIHGMLTLWREGYDVVYGTRTDRPGESLFKRVTARLFYRVLSRLSDVPIPLDT
                     GDFRLMDRRVADTLRAMPERDRFIRGMVSWVGFRQTALPYQRAERLAGESKYPLRRML
                     RFATDGILSFSTKPLQISIALGMFAAFLSVLGVLYALYMRLFTHIWVEGWTALMIAIL
                     FLGGTQLISVGILGEYIGRIYAEIKQRPLYIVQEYWGFDDAEPVLSRSPVPGRR"
     gene            complement(5683..6426)
                     /locus_tag="PLH85_14260"
     CDS             complement(5683..6426)
                     /locus_tag="PLH85_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB65777.1"
                     /translation="MQKIVIVGATSAMAEHCARLWAAQGPVDFTLVVRDAHKATAIAD
                     DLRVRSPGGRVRVLVADFLTPAGIRAAVDQAAAGTPIDIALIAHGSLPDQKACETDLS
                     ACNEALQVNGVSPALFAEAFAGTMLAAGRGTLALIGSVAGDRGRKSNYAYGAAKGLVT
                     RYAQGLQHRLAGTPIKVVLIKPGPTETPMTAHLAATGMRMAPVEDVARDIVAAVTAGA
                     PVAYTPGKWRIIMMVIRHLPAFIFNKMNI"
     gene            complement(6429..7727)
                     /locus_tag="PLH85_14265"
     CDS             complement(6429..7727)
                     /locus_tag="PLH85_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB65778.1"
                     /translation="MSTPVHSWGRLSAAPHDVHSLGDRHRMAETLVRHTPGVAFGNGR
                     SYGDVGLNPGGHLWKTGQLDHFIEFDDASGLLRCEAGTLLRDIQQLAVPRGWILPVTP
                     GTQLVTVGGAIANDVHGKNHHVLGSFGDHVRSLTLVRTTGESIVCGPDVQPEWLAATV
                     GGLGLTGVIAEATLQLRRIASPWLDTETTPYGNLDEFFALADAAEARWEHTVSWIDCL
                     SGRGKGIFMSANPAPPQNREMPGAHRRSMPFTPPISLVNRLTLRPFNLAYYQLQKFKA
                     GRGVAHYEPFFYPLDNLLEWNRMYGPQGFYQFQSVVPRDVGRDAVAAMLQAIERSGKG
                     SFLAVLKTFGDRQAVGMLSFPRPGVTLALDFPNQGHATLKLLAGLDAIVREAGGRIYP
                     AKDARMPRDLFESGYPRLGEFLPFRDPGISSGLSRRLIGS"
     gene            complement(7724..8125)
                     /locus_tag="PLH85_14270"
     CDS             complement(7724..8125)
                     /locus_tag="PLH85_14270"
                     /inference="COORDINATES: protein motif:HMM:NF037976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNB65779.1"
                     /translation="MKLALTYSILALIATCVNIGTQDLANRAYEGEFSLLLSIVAGTG
                     TGLIVKYILDKRYIFSFKARDAAHDGKTFLLYTLMGLITTVIFWGFEFGFDRLFQTRE
                     MRYLGGIVGLAIGYYAKYRLDKRFVFRSDPA"
     gene            8182..9069
                     /locus_tag="PLH85_14275"
     CDS             8182..9069
                     /locus_tag="PLH85_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNB65780.1"
                     /translation="MHDSVAAVSLLGRIRGLLALMRPRQWVKNGFVMAPLVFSGAFGD
                     PLAARHAILAALLFCVASSATYIVNDMHDIERDRRHPKKSRTRPLASGIVTVPMALGL
                     LVLLYGVLVVGWFAMPAVLVVILAYLGLNVAYTFVLKHQPVVDIFTIAIGFVLRVYAG
                     AMALAVPVSSWMFVTTLCLALYLASVKRRQELVGSGSEGRKVLEKYSVGLVDRYAEMS
                     ATGALVFYSMFVMAARPQLVITVPLVLFGLFRYWFVVEQLDGGESPTDALLEDWQLLA
                     TLALWVGACMWVLWPAAGA"
     gene            9072..9488
                     /locus_tag="PLH85_14280"
     CDS             9072..9488
                     /locus_tag="PLH85_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNB65781.1"
                     /translation="MDISYAATPFLAWLVAGGLKFCINSLRAGKPAFALIGYGGMPSN
                     HSAIVSSMAALIAFRQGIGDPAFGVALSLAFVVMLDANSLRRQVGRHAEAINRLTMTD
                     ARHVPLRERMGHTRAEILGGVVTGCVVAWVVSRLSA"
     gene            9485..10444
                     /locus_tag="PLH85_14285"
     CDS             9485..10444
                     /locus_tag="PLH85_14285"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB65782.1"
                     /translation="MMVAPERRRLRFRVPVRSLALLVIVIVGGGAYALLRGDGPAFLG
                     ALTRIPPGTWLSVAGLALISYGLRAVRWMGMLGHLGYSLPRGAGMTVYIAGFALTLTP
                     AKAGETIRAMYLLPWGVPVGASVAAFVAERLMDLVAVTGLSVLAGWGGQGFTWAVPVV
                     AAGGAAILMLVRSRLLPDVVGRIMGRAAGDALIQAVGPLHALLRLQVWMRWVGPTLLA
                     WGAQGVAFHLVVTGLGHAGPLPGLVGLYCLAILLGALSFMPGGLGATEAALVILLERH
                     GLGVADAVAAGLVTRGLTLWLAVLLGSLATARLAVVRSQASEG"
     gene            10480..13173
                     /gene="pepN"
                     /locus_tag="PLH85_14290"
     CDS             10480..13173
                     /gene="pepN"
                     /locus_tag="PLH85_14290"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNB65783.1"
                     /translation="MRTQQGTPILRSAYQPLAYGVESVELDFVLDPTATQVTSRLRCV
                     RHPDAAAGPIVLYGEALERVSLTVDGMAPAPGQLRDTASGLEIDVVGDVVNLEIVTRI
                     DPAANTTLSGLYQSRGGFFTQCEAEGFRRITYFPDRPDVMARFTVRLEADKATCPVLL
                     SNGNLLETGELPGGRHFARWEDPFPKPSYLFALVAADLQALERRVTTMSGREVLLQVW
                     VEARDLDRVEHAMNSLVHSMRWDEEVFGLELDLDRFMIVAVSDFNMGAMENKGLNIFN
                     TKYVLANPETGTDLDFENVESVVAHEYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQQF
                     TADMMARAAESPAAAASARAVTRIANVRVLRNGQFPEDAGPMAHPIRPDSYQEINNFY
                     TATVYQKGAEVIRMIHTLLGREGFRNGMDLYFSYHDGQAVTCDDFVQCMAEANGADLD
                     QFMRWYAQAGTPRVKASGEYDAATGSYTLTLSQHTPATPGQPDKLPLHIPVMLGLIGP
                     DGRDLPLRLEGETRVGETGRLLHLRSDSQRWRFIGLGAEPVPSVLRGFSAPVILELDE
                     SDERLAFRMAHDSDPFNRWDAAQRYMERVILDLAAASADGRTMDVPQAFGHAFRALLI
                     DESLDPAFVAEALSLPSEPYLLERMAPADPEPLRAALIHLMRELGQAMQADFLSRYLD
                     LEVDGDYRYHPSDAGRRALRNLCLRYLMAWGGATAVELAVRQFEFAGNMTDRYGALAA
                     LVQSDSPRRAEALAAFHDRYRDDALVLDKWFALQAGAWRWSDAAPQTLDQVRTLMDHP
                     AFSLGNPNKVYALLGGYFRANPGEFHRADGLGYAFWADQVVILDRANPQVASRMARAL
                     EGWRKFTPAIQDGIRAALERVAGSAGLSPDVSEVVGKALKG"
BASE COUNT         2439 a         3898 c         4465 g         2509 t
ORIGIN      
        1 cagttcgccc gccaagcggg ccgccgcgta gtcaccgcgc atccgctcaa gcatcgtaag
       61 cccccgggca tcccatcttc ccccagccct ttagctcgct aaacatagtg tccactagcc
      121 aatagaagtg gacacttaca tgaacgtcac gaagcctcgc cgaacccgtc gcacccactc
      181 cgaggagttc aagcgttccc tgatcgaagc ctgcggggag cccggcgcgt cggtcgcggg
      241 tgtcgcgctg gcgaacggga tcaacgccaa ccagctgcgg cgctggatgc gcgagcgggg
      301 catcgatcca ccggcgttgt gaagttgaag ttgaaggagc gttcagcccg cgccgggaca
      361 ggggcgggct gattgttcag gaccgacggg ttacaacgtg cgctggctgc tgcgggcggt
      421 ggccggcttg gggctgaagg ctctttttgc gctctgcgtg tgggttggga tgctggggcg
      481 ggtggtcagt ggcaacgctg cccggccgct tggcgggggg ctgctaactt cactgttcag
      541 cgcactcggc tttcctgtac aggcggtatt gatagttgta gggtgtttcc agcccgcctg
      601 tttgtgcaac ggggattgct gtgtagcgtt gtttaatcaa tgcttcaatt ttctcaatac
      661 gcccgccgat gttgggggac catccgtcgc cgcccacgta aacaatgaag gacgccgggt
      721 tggctgtcag tgagtccacg agttgacgtt gacgatccgc gaaaaggctt ccgtccacga
      781 ctgggttgag aaagacagtc caggaatagg ggcggatgcc gacggccgct gcgtgggtat
      841 agatctgagc gcgattacca tagacgagta cgtcatttgg cacgcccttg caattctgtg
      901 cactcatgat ttgggcgatg gcgttcgttt tctgagtgtg ctggctgacg agagggttct
      961 gtaaaatgcg cagatccaat ttgtgggcgc tatatccgag cgttccagca agtatcgtgg
     1021 agagcagtac cgcggcgatt gtgcggaagg tctgtgtcgc atttaatgca gagtaggtga
     1081 aggcgagcag caaaggcagg atgaagccaa agagataaaa cgactggatt tttcgttgaa
     1141 atacgatgga agccacttcc gtgagtgccc atgcccacag gacgaggctg gtcgaacgat
     1201 ggggcgatgt tgcgcaggat aaggatgcga cgccgctggt caggaggact ccagccaggc
     1261 acagggcaat cgggtcctga aaaaagaaat ccgctacggc ggttgtgctg cgtgcgcggc
     1321 ttccgtagtc tgcgttgtag tgaatgagac tgtcgaatac cgccgagccg ccgaatgtca
     1381 gaagccaaag agcacatcct ccggccacca tgactgcgcc cgagagcgcc agccagagtc
     1441 tacgccgttc gccggttttc cgatattcga gaaaaagcag cgtgagcatg aaaggcaatg
     1501 cggtcagcgc ggtgggtcgc aggaaaggcg ccagcccaat cagtgcgcca cccagaaata
     1561 cagtggtgtc gcgtttgcca tcggcgcaga gtacgagccc cgaaaatgca catatcaggg
     1621 cactgaccat caggatcccg tttgccacag gatcaagcca gccgaggacc aggctggtgg
     1681 ctacagcaga ccccagggcg gcgagggtgc ggttgtcggt gggccgtgcg atggaaagag
     1741 cgcgataggc gagaagcgtc aatccggagt acgcgcacaa cgcgcctaac ttcaatccgg
     1801 acgtatcgcc gagcaatgcg tccagatggc tgaaaatagc gaagtagaga tagatgaggg
     1861 ggggcttgtg atcccagtaa tttccccaga gttcaagtcg tccgtccgtt gcgagacgtc
     1921 ggccgatctc gtaataaacg ccgatgtcgg ttccgacaaa ctcatacagt tgtggcaggt
     1981 aggctgtcag gacagtcgcc agcaaagccg cgatgtgcag gaggccgacg gattcgaatc
     2041 gtttcatggg acttcgcttg cgaatgaggc tgaatgcgat gtggtgcgcc ggcatgcaat
     2101 gattgcttcg gccaggcggc ggccttcccg cagtgcgtaa ttggttccac ggtcttcggg
     2161 atagatctgt gccatggagc agacgtgaaa tccctgccgg ggaccttggg ttggcggaat
     2221 gagcctgctg taaaagcgtt caacaacggg ttgggaccag cgtgcccgcc atagatggtg
     2281 agccacgacc cattcgcggc gaaatgccgg aaacatcgtc tggatatagg gtagggcgta
     2341 cgccaggaaa ctgtctgcgt ccagaccaaa cagggtgtcg gtgacgggca gatacttgga
     2401 aagatagacg atgtgccgcc cgccgtaggt ttccggtcgt gcgaagttgg tatgttcaat
     2461 gatgccgacg aagggaaagc tggggtcgtt gacgttcagc cagtaggtgt ctgacaagga
     2521 gcgatcgagt tcgagcacca tgcacacgtt gccgagatag cggattcgcc gcaggcctgc
     2581 cagatagtct gcgggggccc atgtgtcgat catgtcggcg atcaggggaa gcgccacggt
     2641 ggcggcgact tcgtgggccc ggaactggcc tgccgtggtc tcgagctgcc attggtttcc
     2701 gtccggacga acgccggtca ccctggcatt ccggacaatc cgtccgcctg ctgcgacgat
     2761 gtgcctggca agcgcctcgg ccagtgcaac gaacccccct tcgaaatacg cgagtcgttc
     2821 ttccccggcc ttgccgcgac tgccgccgcg aagcttgagc ttgttccaga accataccgc
     2881 cgacacctcg tcggcgtagg ggccgaactt gccttgaagc aggggttgcc agaccacgcg
     2941 atagacgtcc tcgccaccca aggcacgaag ccactcatgg gcagtcaatg actccaggct
     3001 gtgccagtcc ctgaccctgc gtgcccgtaa cgcgagcagc cccaggcgca ggcgcgcgag
     3061 cagacccagc ggcttgaagc acaggagatc cagtggagta gacaggcgga agaactggtg
     3121 ggcgtaatag atcccggtat tggttggatt gacggtgact ttgtcgctga gcccaagttc
     3181 ttcgatcagg gtcatgacct cccggtcatt ggtgaaccag tgatgataga aacggtccag
     3241 cgtctgcccg ccaacaggga aagcggccgc gagcccgccg atgtccggct cggcttcgag
     3301 gaccgtgacg ggcacgcctg cctgtaccag acggtaggcg atggacaggc cggtgaagcc
     3361 tgccccgata atggcgatgg gcgcggtgtt catggtgcat ttccctggcg cctggcgctg
     3421 acaccgacag gccgtgcgat cacgtagcat ccccccgcga gggtcaccgg accccacagg
     3481 atggcatgca cgaagaaggc gtaggttgcg gatgcggtcg cggtattgcc ggcacttgaa
     3541 agtgcccagg cggtgaaata gtcgaacgtg cccagatagc cgggcgaact gggcagcaag
     3601 gtcgcaagcg tgccaagcgg gagcgcgagc caggctgccg ccggttgggt gatggccggg
     3661 agcgctagcg cacagcagta gaagaccagt ccttcgatca gccaggcgag catggaccac
     3721 atcagcaaac gaagcgcgag ccggggttgc gcggactgtg tcagaagcgt gaagacccgt
     3781 gcggattccc ggtcaagccg ctgcccgagt gaagcatgcc agcgggtgac gaagctgacg
     3841 atgatcttga cgggaggatg gatcgccgtc ggacgcagca gcaggccaag aatcaggctc
     3901 gcacctgcaa gaaagaccgc agtgcctgca cccatcagac gggaaatatc cagacccagc
     3961 gcgacaggca acaccccgag aatgatcaaa agcacgagca aatccagcag gcgctcctga
     4021 aacaggcttg caaaggcggt ggtcgtcccg atgcccagac gggcattgaa accgaacaga
     4081 cgcagcagat caccgaggcg aaagggcagc acattgttgg ctgccacgct ggccaacaag
     4141 gggcccgcgc aatgccgcca gcgcagggtg gggttgtcgt gacacagcaa caggcgccag
     4201 cgctcgatcc ggcaggcgta accgcagaaa aacgctgcga tcgcgagggc tgtccagtcc
     4261 gggcgtgctc cgccaagaat ccggagcatt tcgtccgaat cgacctgccg caggatcagc
     4321 cacaggaaga gcccggcgag acccagtccg agcagggtac gcgaggttcg ccgcatgctc
     4381 agcggcgccc cgggacagga ctgcgcgaca gcacgggttc cgcatcgtca aagccccagt
     4441 actcctgaac gatgtagagg gggcgctgct tgatctccgc gtagatccgg ccaatgtact
     4501 cgccaaggat gcccacgctg atgagctgtg ttcccccaag aaacaggatc gcaatcatca
     4561 gggccgtcca gccctctacc cagatgtggg tgaagaggcg catgtacagg gcgtagagca
     4621 cacccagaac ggacaggaag gcagcaaaca tcccgagggc gatggagatc tgcaagggtt
     4681 tggtcgaaaa ggacagaatg ccgtcagtgg cgaatcggag catccttcgc agcgggtatt
     4741 tgctctctcc ggcgagtcgc tcggcgcgct ggtagggtag cgctgtctgg cgaaatccga
     4801 cccagctgac catgccacgg atgaagcgat cgcgctccgg cattgcccgc aaggtgtcgg
     4861 ccacgcgtcg atccatcagc cggaagtccc ccgtgtcgag gggaatcgga acgtcagaca
     4921 atcggctcag cactcggtag aacagtcggg ccgtcacgcg cttgaacaag gattcaccag
     4981 ggcgatcggt gcgcgtgccg taaacgacat cgtagccctc ccgccagagg gtcagcatcc
     5041 cgtgaatgac ctccggcggg tcctggaggt cggcatcgat gagtaccacg gcgtcgcccc
     5101 gtgccgcatc aatacccgcc gtcacggcca cctggtgccc gaaattacgt gcaaaaccga
     5161 tgatccggat acgtggatct tctgtcccga atgatttcag acgggctcgg gtgtcatcct
     5221 gactgccgtc atcgacaaag atcaattcca cccggacgtc cttgaccgtc gcgcagaagg
     5281 ctctgagacg attcacggta ctggtaatga cgtcctcttc gttgaagcac ggaatgacaa
     5341 tggacaggag cggcgtgtcg gatgtgtcca tttgcgtcgt catggtggcc agcgggaagc
     5401 gtgtgatggg ggcggggcgg accagcttcg gaagagattt ccgagaaaca tctccagccc
     5461 gtcagcaagc agacgccctg ggcgacatat cgggtgatgc ccgctgctgg atcgccggtc
     5521 gtgcgatggg actcggcctg aaaatcgggt gtgaccttgt ggggagtgtc cgttcaacga
     5581 ttgaaaaagt atatcagccg agaagacgct gcatttagag ggctatcggt ggcgccgtct
     5641 gatcggcgcg ggttcgagcc gatccagtcg cttgcagtgt gctcagatgt tcatcttgtt
     5701 aaagataaac gccggcaaat ggcggatgac catcatgatg atccgccatt tgccgggtgt
     5761 gtacgcaacg ggagcgcctg ccgtcacggc agcgacgatg tcgcgcgcca cgtcttcgac
     5821 cggcgccatg cgcatgcccg tagccgcgag gtgggccgtc atgggcgttt cggtagggcc
     5881 gggcttgatc aggacgacct tgatcggcgt gcctgccagg cgatgctgga ggccttgggc
     5941 gtaacgtgtc acgaggccct tggcggcgcc ataggcgtag ttggatttgc gcccccggtc
     6001 tccggcgacc gagccgatca aggccagggt gcctctgccg gctgccagca tcgtcccggc
     6061 aaacgcttcg gcaaacagtg ccggcgatac gccattgacc tgaagggcct cattgcaggc
     6121 agacagatcc gtttcacagg ctttctggtc cggcagggat ccgtgcgcaa tcagcgcgat
     6181 gtcgatcggc gttccggccg ccgcctgatc caccgcagcg cgtatacctg ccggagtgag
     6241 gaagtcggcg acgagaacgc gtacccgacc gcccggactg cgtacccgga ggtcgtcggc
     6301 gattgccgtg gccttgtgcg cgtcgcggac gaccagggtg aagtcgaccg gaccctgggc
     6361 ggcccacagg cgggcacagt gttcggccat cgccgaggtc gcgccgacaa tgacgatttt
     6421 ttgcatgatc aggatccgat gaggcggcgg gacaatcctg aactgatgcc cgggtcgcga
     6481 aatggcagga attcgcccag acgtggatag ccggactcga acaggtcgcg gggcatgcgg
     6541 gcgtccttgg cggggtagat gcggccgccg gcctcgcgga cgatggcgtc cagaccggca
     6601 agcaatttca gcgtggcatg tccctggttg gggaaatcga gggcgagggt cacgccaggg
     6661 cggggaaagc tcagcattcc aaccgcctga cggtcgccga aggtcttcag tacggcgagg
     6721 aaagagcctt ttcccgagcg ctcgatggcc tgcaacatgg cagccacggc gtcccgaccg
     6781 acgtcacggg gaacgacgct ctgaaactgg tagaagccct gcggtccgta catcctgttc
     6841 cattcgagaa ggttgtcgag gggatagaag aatggctcgt aatgggcgac gccccgaccc
     6901 gccttgaact tttgcagctg gtaatacgcc aggttgaagg ggcgcagggt aagcctgttg
     6961 acgagcgaaa tcggcggcgt gaacggcatg ctccgacgat gggcgccagg catctcgcgg
     7021 ttctggggtg gcgccgggtt ggcgctcatg aagatgccct tgccacgacc agaaagacag
     7081 tcgatccagg agacggtgtg ctcccagcgc gcctcggcgg cgtcggccag tgcgaagaac
     7141 tcgtccaggt tgccgtaagg ggtggtttcc gtgtccagcc aggggctggc gatacggcgc
     7201 agctgaagcg tggcctcggc aatgacgccg gtcaggccca gtccgcccac cgtggcagcg
     7261 agccactccg gctgcacgtc cgggccgcag acgatggatt caccggtggt ccggacgagg
     7321 gtcaggctgc gtacgtgatc gccgaaggat cccaggacgt ggtgattctt gccatgcacg
     7381 tcgttggcga tggcgccacc gaccgtgacc agttgggtgc ccggcgtgac cggaagaatc
     7441 cagccccgcg gcacggcgag ttgctgaatg tcccgcagca aggtgccggc ttcgcatctg
     7501 aggaggccac ttgcgtcgtc gaactcgatg aaatgatcca gctggcctgt cttccagagg
     7561 tggccgccgg gattcaggcc cacgtcgccg tagctgcgcc cgttgccgaa ggccacgccc
     7621 ggggtgtggc ggaccagcgt ttcggccatc cggtgacgat cacccaggct atgcacgtcg
     7681 tggggggctg ccgacagacg cccccacgaa tgaacgggcg tactcatgca ggatccgaac
     7741 ggaatacgaa gcgcttgtcg agccgatatt tggcgtaata cccgatggcc aatccgacga
     7801 ttccacccaa atagcgcatc tctcgagtct ggaataagcg gtcaaatccg aattcgaaac
     7861 cccagaaaat gacggtggtg atcagaccca tcagggtgta gagcaggaag gtcttgccat
     7921 catgggcggc gtctcgcgcc ttgaacgaaa aaatgtaacg cttatcaagt atgtacttga
     7981 cgatcagacc ggttccggtg cccgccacga tcgaaaggag cagggaaaac tccccctcat
     8041 aggcccggtt ggcaagatct tgggtgccga tgtttacaca ggtcgcgatc aacgcaagga
     8101 ttgaataggt cagcgcgagt ttcatcgggc gagtggcgcg tggtataacg acgcgtcttt
     8161 tgattctgaa tcgtggtatc catgcatgat agcgtagcgg cggtctctct cctaggccgc
     8221 atccgtggcc tcctggccct catgcggccg cgtcagtggg tgaaaaatgg cttcgtcatg
     8281 gctccactgg tgttttcggg tgcctttggc gatccgctgg cggcgcgtca tgcgatcctc
     8341 gcagccctcc tgttctgtgt ggcgtcttcg gcgacctaca tcgtcaatga catgcatgac
     8401 atcgagcggg atcgtcggca tccgaagaaa tcgcgcacgc ggccgctggc ttcgggaatc
     8461 gtcaccgtac ccatggcgct ggggctgctc gtactgttgt acggtgtgct cgtcgttggg
     8521 tggttcgcga tgccggcggt gctggtggtc atcctggcct acctcggcct caatgtggcc
     8581 tatacctttg tgctcaagca ccagcccgtc gtggatatct tcaccattgc catcggcttc
     8641 gtgcttcggg tctacgcggg tgccatggcg cttgcggtgc ccgtgtccag ctggatgttc
     8701 gtcacgaccc tgtgtctggc tctgtacctc gcgtcggtca agcgtcgtca ggagcttgtc
     8761 gggagtggca gcgaggggcg caaggtgctc gagaagtatt ccgttgggct cgtcgatcga
     8821 tacgcggaga tgtcggccac cggtgcgctg gtgttctaca gcatgttcgt gatggcggcc
     8881 cgtccgcagc tggtgatcac cgtacctctg gtcctgttcg gactgttcag atactggttc
     8941 gtggtcgaac agctcgatgg cggtgagtct ccgacggatg cgctgctcga ggactggcag
     9001 ttgttggcaa ccctggcctt gtgggtgggg gcctgcatgt gggtcttgtg gccagcggct
     9061 ggggcgtgac catggatatc tcgtatgcgg caacgccctt tctcgcctgg ctggtggcgg
     9121 gcggtctgaa gttctgcatc aacagcctgc gggccggcaa gcctgctttt gccctgatcg
     9181 gctatggcgg gatgcccagc aaccacagtg caatcgtcag cagcatggcg gccctgatcg
     9241 cctttcggca gggcattggc gacccagcgt tcggtgtggc cctgagcctg gccttcgtcg
     9301 tgatgctgga cgccaacagt ctgcgccgcc aggtcggacg ccacgccgaa gcgatcaatc
     9361 ggcttaccat gaccgatgca cggcacgtgc cgctacggga gcgtatgggc catacccggg
     9421 cggagattct cgggggcgtc gtgaccgggt gcgtggtggc ctgggtggtg agtcgcctgt
     9481 cggcatgatg gtcgcacccg agcgtcgtcg tttgcgcttc cgggttcctg ttcgcagtct
     9541 ggccctgctg gtgatcgtga tcgtcggcgg cggcgcttac gcactcctgc ggggagatgg
     9601 cccggcgttt ctcggggcct tgacgcgtat tccaccggga acgtggctga gtgtcgccgg
     9661 tctggcgctg atcagttatg gactgcgtgc ggtccgctgg atggggatgc tggggcatct
     9721 gggctactct ttgccccgtg gcgccggaat gacggtctac atcgccggct ttgcccttac
     9781 cctgacgccc gccaaggcag gcgaaacgat ccgcgcgatg tacctgctgc cctggggggt
     9841 gccggtcggg gccagtgtgg ctgcctttgt tgcagagcgc ctgatggatc tggtggccgt
     9901 gacaggattg tcggtgcttg cagggtgggg cgggcaggga ttcacctggg ccgtgcccgt
     9961 ggtcgcagcg ggcggcgcgg cgatcctgat gctggttcga tcgcgcctgt tgcccgatgt
    10021 cgtgggtcgc atcatggggc gagccgcggg tgacgcattg atacaggctg ttgggccgtt
    10081 gcatgcgctc ctgcgcctgc aggtctggat gcgctgggtc gggccgacgc tgctggcctg
    10141 gggcgcgcaa ggggtcgcat tccacctggt ggtgaccggg ttggggcatg cgggcccgct
    10201 gcctggtctg gtgggactgt attgtcttgc gatcctgctc ggggcgctgt ccttcatgcc
    10261 tggcgggctg ggggcgaccg aggcggcact cgtgatcttg cttgagcggc acggactggg
    10321 ggtggccgac gcagtcgccg cggggctcgt gacccgtggg ttgacgctct ggctggcggt
    10381 tctgttgggc agtctcgcca cggcccggct ggcggtggtc cgcagtcagg cctcggaggg
    10441 ctagaatgcc ggctgacacg aactgatcag gactcctctg tgcgcaccca acaaggcacc
    10501 cccatcctcc gctctgccta tcaacccctc gcatatggcg tggaatcggt cgaactcgat
    10561 ttcgtgctcg atcccaccgc aacgcaggtc accagtcgtc tgcgttgtgt acgtcatcct
    10621 gacgcggcgg ccgggccgat cgttttgtac ggcgaggcgc tggagcgggt gtccttgacg
    10681 gtcgacggta tggcgcccgc accggggcag ctgcgcgaca ccgcgtccgg gcttgaaatt
    10741 gacgtggtgg gcgatgtggt gaacctcgag atcgtcaccc gcatcgatcc ggccgccaat
    10801 accacgctgt cgggccttta ccagtcgcgc ggaggttttt tcacccagtg cgaggcggaa
    10861 ggtttccgcc gcatcacgta tttccccgac cgtccggacg tgatggcgcg cttcaccgtg
    10921 cggctggagg cggacaaggc gacatgtccg gtcctgctgt ccaacggcaa tctgctcgag
    10981 accggcgaac tgcccggcgg tcgtcacttc gcccgttggg aggatccctt ccccaagccg
    11041 tcctacctgt tcgccctggt ggcggcggat ctgcaggcac tggaacgacg tgtgaccacg
    11101 atgagcgggc gcgaggtgct gctgcaggtg tgggtggagg cccgtgacct ggaccgcgtc
    11161 gagcatgcga tgaattccct ggtgcactcg atgcgctggg acgaggaggt cttcgggctc
    11221 gagctggatc tggaccgctt catgatcgtc gcggtgtcgg acttcaacat gggggccatg
    11281 gaaaacaagg gcctcaacat cttcaacacc aagtatgtgc tggccaatcc agagaccggc
    11341 accgacctgg atttcgagaa cgtggaaagc gtggtcgccc acgaatattt ccacaactgg
    11401 accggcaacc gggtcacctg tcgcgactgg ttccagctca ccctgaagga agggctcacc
    11461 gtcttccgcg atcagcagtt tacggctgac atgatggcgc gtgccgccga gtcgccggcg
    11521 gccgctgcgt cggcgcgggc ggtgacgcgc atcgccaatg tgcgcgtcct gcgtaatggc
    11581 cagtttcccg aggatgcggg gccgatggcc catccgatcc ggcccgacag ttaccaggaa
    11641 atcaacaact tctacaccgc cacggtttac cagaagggcg ccgaggtgat ccgcatgatc
    11701 cacaccctgc tgggccgcga aggcttccgc aacggcatgg atctgtattt ctcgtaccac
    11761 gacggccagg ccgtgacctg cgacgacttc gtgcagtgca tggccgaggc caatggtgcg
    11821 gatcttgacc agttcatgcg ttggtatgcc caggccggca cgccgcgggt caaggcgtcg
    11881 ggcgaatacg atgcggcgac cgggtcctac accctcaccc tgtcccagca caccccggcg
    11941 acgcccgggc agccggacaa gctgcccctg cacattcccg tgatgctggg gctgattggt
    12001 ccggatggcc gcgatctgcc cctgcgcctc gaaggcgaga cgcgggtcgg cgagacgggc
    12061 cgtctgctcc atctgcgctc ggacagccag cgctggcgct tcatcgggtt gggcgcagag
    12121 ccggtgccgt ccgtgctgcg gggattctcc gcgccggtga tccttgaact cgacgagtcc
    12181 gacgagcgcc tggccttccg catggcccat gacagcgatc ccttcaatcg ctgggacgcc
    12241 gcgcagcgct atatggaacg tgtcatcctc gatctggccg ccgcgtccgc cgacggccgg
    12301 acgatggacg tgccacaggc tttcggacat gccttccgcg ccttgctgat cgacgaatcc
    12361 ctcgatccgg cattcgtcgc ggaggccctc agcctgccct ccgagcccta cctgctggag
    12421 cgcatggcgc ctgccgatcc cgagcccctg cgggccgcgc tgatccatct gatgcgtgag
    12481 ctgggtcagg ccatgcaggc tgatttcctg tcccgctatc tggatctgga ggtggacggt
    12541 gactatcgct atcacccgtc cgacgccggc cggcgtgccc tgcgcaacct gtgcctgcgt
    12601 tacctcatgg cgtggggcgg ggccacggcg gtggaactgg ccgtgcggca gttcgagttt
    12661 gcgggcaaca tgacggaccg ctacggggcc ctggcagcgc tggtgcagag cgattctccg
    12721 cgccgtgctg aagccctggc tgctttccat gaccgctatc gggacgacgc cctggtactc
    12781 gacaaatggt ttgcgctgca ggctggcgca tggcgctggt cggacgccgc gccgcagact
    12841 ctggatcagg tccgtacgct gatggatcat ccggccttca gtttgggcaa tccaaacaag
    12901 gtttatgcct tgttgggggg gtatttccgc gccaatcccg gagagttcca tcgggcagat
    12961 ggcctgggtt atgccttctg ggccgatcag gtggtgattc tcgaccgtgc caatccccag
    13021 gtagcctccc gcatggcgcg ggcactggaa ggctggcgaa aattcacgcc cgccatccag
    13081 gacgggatcc gcgcggcact ggagcgggtg gcgggaagcg cagggctttc gccggatgtg
    13141 tcggaagtgg tcggaaaggc gttgaagggc tgatgtaatt ccttcgatgt ctgaaagtga
    13201 aaaggccgcc ctgaggggcg gccttttcat gggcggtgcg gcgcgtcaag gggtgaaggt
    13261 gatctccagc ttgggccgca gggaagcggt ggcgtactca cgggatgcga a
//
LOCUS       DAOQMM010000105        12874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2078_length_12874_cov_47.974257, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000105 DAOQMM010000000
VERSION     DAOQMM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2078_length_12874_cov_47.974257"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLH85_14295"
     CDS             <1..782
                     /locus_tag="PLH85_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNB65784.1"
                     /translation="EQMLKQFIKKVHDTRLFSPSPRIIICVPCGSTQVERRAIRESAL
                     GAGASNVYLIEEPMAAAIGAGMPVADATGSMVVDIGGGTTEVGVISLGGMVYAGSIRV
                     GGDKFDEAIVNYIRRNYGMLIGETTAESIKKEIGSAFPGSEVKEMEVKGRNLAEGIPR
                     AFTISSNEILEALTEPLNQIVSAVKIALEQTPPELGADIAERGMVLTGGGALLRDLDR
                     LLMEETGLPVIVAEDPLTCVVRGSGIALEKMDQLGSIFTSE"
     gene            893..1807
                     /gene="mreC"
                     /locus_tag="PLH85_14300"
     CDS             893..1807
                     /gene="mreC"
                     /locus_tag="PLH85_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNB65785.1"
                     /translation="MVGHSPPPFFKRGPAPLAKLVFFGVLSLAMLVSDLNLRYLDAFR
                     QGLSVLAYPLQMAAATPADFVRNASDYFASLVRLQRENSELKRKQLAGAERLLRQDQL
                     EQENRHLRTLLDARERQPVKSVIAEILYTAKDPFSRKVILDKGAQAGIEAGQAVVDDL
                     GVIGQITRVFPMQAELTLLTDKDQAIPVMIVRNGLRAMLFGAGNGLMELRYLAANADV
                     QPGDKVVTSGLDGVFIAGLPVATVARVDRDQSQTFARIPCLPAAGVEQFGHVLVLGHR
                     ESVAPRPPEPDPLPGKAGKGRKARLAKE"
     gene            1811..2332
                     /gene="mreD"
                     /locus_tag="PLH85_14305"
     CDS             1811..2332
                     /gene="mreD"
                     /locus_tag="PLH85_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNB65786.1"
                     /translation="MQPTNRSSRILLPVKMWFVFLTLFIALFLNFIPTGHLPAVPDFV
                     ALVLAFWCIREPLRIGIGTGFVLGVLTDIGVGAAMGQYALAYVLLAYAANELSRRVLW
                     FTPVEQALHVLPLLFLSQVVMVVVRVIAGADFPGWWMFFGSFVGALLWIPLHYVLLLP
                     QYQPVERDENRPI"
     gene            2405..4309
                     /gene="mrdA"
                     /locus_tag="PLH85_14310"
     CDS             2405..4309
                     /gene="mrdA"
                     /locus_tag="PLH85_14310"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB65787.1"
                     /translation="MKNVRTPEQDLARFRGRLAVAGVVALVCFGLLVARFFYLQVVRY
                     DYYSTRAEDNRISLVPIVPNRGLIVDRNGTVLARNYSAYTLEITPSRVADLEATIEAL
                     SKLVDIQPKDRKRLKKLMDESKSFESLPIRNRLSDEEVARFIAQRYRFPGVELKARLF
                     RDYPLGSFASHIIGYIGRINDRDLERIEDNDDTANYRGTDHIGKAGLEKSYERELHGV
                     TGFEEVEVDSGGRAIRSLRRNPATPGNNLILTVDLKLQEVAEKAFGNRRGALVAIEPG
                     SGGVLALVSVPTFDPNLFVDGIAPQDWDELNTSPDHPMVNRALNGAYPPGSTFKPFMA
                     LAALMSGKRTAAQAIADPGFFDFGGHHFRDDKPGGHGTVDMYRSIVQSCDTYYYRLAN
                     EMGIELIAATVGQFGFGARTGIDIEGESVGVLPSPEWKKRRFKKPEQQKWFGGETISV
                     GIGQGYNAYTMLQLAHATATVAAGGVMYKPHLVKFIENAKDGEKRVIEPEPIKTIPIR
                     PEHIETIRRAMVGVNREGTGARAFAGAPYAAAGKTGTAQVFSLKGAKYSAKIAERLRD
                     HALFIAFAPADKPTIALAVLVENGGFGAESAAPIARQVLDYYLLGKLPASVAPEYPGA
                     EGVPEERPVE"
     gene            4302..5444
                     /gene="rodA"
                     /locus_tag="PLH85_14315"
     CDS             4302..5444
                     /gene="rodA"
                     /locus_tag="PLH85_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNB65788.1"
                     /translation="MSEFRFQPLRWIARLLAPLDGPLVTIVLAILCFSTIVMVSASPE
                     RLDTLALNIAVAFTVMWVAASVPPQRMMTIALPLYVLGVLLLIGVALFGDVSKGARRW
                     LNIGVARIQPSELMKIAMPLMLAWYFQSREGMIRVRDFFIAAALLLVPVGLIAKQPDL
                     GTAILVAAAGFYVLFFAGLTWRLIVPVAVVGIVSVAAIVATGDRICQPDVPWPGLRDY
                     QKHRICTLLDPTSDPLGKGFHIIQSTIAIGSGGVLGKGWGKGTQTHLDFLPERHTDFI
                     FAVLTEEFGLAGALILAVLYILLIFRGLFIAAGAPTLFSRLLAGALTLIFFTYAFVNM
                     GMVSGILPVVGVPLPFVSYGGTALVTLCLGVGMLMSIQRHRRRVGT"
     gene            5501..6496
                     /locus_tag="PLH85_14320"
     CDS             5501..6496
                     /locus_tag="PLH85_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNB65789.1"
                     /translation="MTPEAIRRPRRGPCCAIAPVSALLLAFGALLAGTAHAAAFEGMF
                     PSETLHIAPPPDADDAPAAAAPVTYAAPSYALPDRRELAALSLYRERGIASYYGRPFH
                     GRKTSSGDLFDMYAMTAAHPTLPIPSYVRVTNLANGRSVVVRVNDRGPYHPGRAIDLS
                     YTAAFKLGYADQGQANVEIAQVLPEEVAFVQTRPVPPLRRAASRPAPAQVAAAAVPVP
                     AAAPPAPRTESTPVAAPIAPPVATPAVAAAPLADAASSPVFLQLGTFGSLVNAESFKT
                     FVEHELRSVKETVSLLASDGKYRLHLGPFPSAQEARSMAERIATTLKLKPSVVQR"
     gene            6650..6826
                     /locus_tag="PLH85_14325"
     CDS             6650..6826
                     /locus_tag="PLH85_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65790.1"
                     /translation="MPVRPAPQRPKYRPLRRPSSPSATLLDPDGLPDETDLTRIRVRM
                     AAARQQQQRAHVPH"
     gene            7507..7782
                     /locus_tag="PLH85_14330"
     CDS             7507..7782
                     /locus_tag="PLH85_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65791.1"
                     /translation="MTTEARETAVRIGTADIETVAREGVARALAARTQATELSADEID
                     AVSGGLTLSTIQLAQPTLVLKPGILAGPYPVDVLKGGVIAAATVQKY"
     gene            7795..8265
                     /locus_tag="PLH85_14335"
     CDS             7795..8265
                     /locus_tag="PLH85_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65792.1"
                     /translation="MATQPEILCPSAFPEWDGARLFAVVGGTPERPETAYLDQLQPVT
                     PELLELAGPVAPIEVFRFAAPCAERACQQFDGSTGRCQLARKTARIAPVVVHKLPRCG
                     IRANCRWWLQEGPSACKRCPQVATLNYAPSEAIRHAADPARPLDEVGTTAPLGT"
     gene            8291..9124
                     /locus_tag="PLH85_14340"
     CDS             8291..9124
                     /locus_tag="PLH85_14340"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB65793.1"
                     /translation="MSSTRKALCVGINQFKNYPSAALQGCVNDARDMASLLQQLLGFT
                     AADIAVLTDAQATKAAIMAQLKAMVDGAKAGKYSYLVFSLSSHGTQVPDLSGDEPDRA
                     DEAFCPHDLAQKGNQWDPAHIITDDELHDLFVQIPPTVLLEVYLDTCHSGTGLKGIDM
                     LLDRKPRYLPPPSLEAFRQIEGRRSRGIAQAIKEKGPATHNILWAACRPDQTSADAHI
                     AGGWHGAFTYFFTKEMRASKNKLSRAQILTKVRADLAAGGYTQVPQLEVDATTRQLAA
                     A"
     gene            9211..9417
                     /locus_tag="PLH85_14345"
     CDS             9211..9417
                     /locus_tag="PLH85_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65794.1"
                     /translation="MRTPPCPSAPVELSAAEIEAVSGGMGALAILAGAAAFVAAGALV
                     AAVAVTADNAANGPLAGAWYRFTK"
     gene            9483..10730
                     /locus_tag="PLH85_14350"
     CDS             9483..10730
                     /locus_tag="PLH85_14350"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB65795.1"
                     /translation="MRPALFRPEVLAQRPDGRHGEVLIAHPPALILLVALAVAFTAGL
                     AAYAWWGEYTRKEHVSGSLVPTRGLIRVFTPQAGTVLEKAVREGQPVRQGDVLLVLSS
                     ERASTATRETQAAQLAALRQRRDALRAEQAKQTEIDTLSGSAAADRAHGLVKELAEAR
                     AQLDLQRSRVAAAERTVARHEQLVAARFVAEATLQQKQEELLDQRARQAETQRIIAGL
                     ERDLATARAELASAGLRRANTASAITRQISELDQQLTETDSRRSVVITAPADGIVTTL
                     LAEPGQTATPGTPLLSLLPAGAELQAQLLVPTRAAGFIRTGQEVVLRYQAFPYQRFGH
                     HLGQVVEVGRSVIQPADAGQAGSVQEPVYRVTVRLPAQSVQAYGEAMALQAGMTLDAD
                     VRLDRRRLIEWLFDPLLSVTGRI"
     gene            10744..>12874
                     /locus_tag="PLH85_14355"
     CDS             10744..>12874
                     /locus_tag="PLH85_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNB65796.1"
                     /translation="MLDFGFASRTPLILQGESAECGLACLAMVAGHHGHRIDLASLRA
                     RHSLSQRGVTLADLMRIAGTLHLAPRPLRLEPEQLGQLRLPCVLHWDLNHFVVLIRLR
                     GDRASVHDPACGARTLRLAELSRHFTGVALELNPTDAFTPATERRRVRLGQLVGPLRG
                     LGGSVLQALVLALVLQLFALLAPFYMQWVVDQALVTQDRDLVTVLGVGFLLLALVNAG
                     VAALRGWVLMVLGTSLNLAMLTRLFRHLIRLPMTWFDKRHLGDVVSRFESLNSIQRTL
                     TGGFLEALVDGAMALLTLAMMLFYSLPLASVAIVAAAAYAGLRAALYQPQRRAAEAHI
                     VRGARQHSHFLETVRGIQTLRLHAHEEPRTVAWHNLAVDQFNAGIRSQRLGLLYQGLN
                     TLLFGIENVVTVWLGARLVLDTASGAGFSVGMLFAFIAYKNQFVQRMAAFIEKALELR
                     MLGLHAERVGDIALAAAEPDEPPASAAAAPLAGHLSVRNLAFRYGDTDPRVLDDVSFD
                     VRAGESVALVGPSGGGKTTLVKLLLGLMAPESGGIEVDGVPLARFGLRRYREQVASVM
                     QDDQLFAGSLADNIAFFDPQADPTRIEACARLAAVHDDIAAMPMGYHTLVGDMGTVLS
                     GGQKQRVLLARALYRRPRILVLDEATSHLDIARERCVNDAIRGLPLTRLIVAHRPETI
                     ASCDRVIVLTGGRVTNEMYAVAGGMGGA"
BASE COUNT         1996 a         4951 c         3924 g         2003 t
ORIGIN      
        1 ccgagcagat gctcaagcag ttcatcaaga aggtccacga cacccgcctg ttctccccca
       61 gcccgcgcat catcatctgc gtgccctgcg gctcgaccca ggtggaacgc cgcgccatcc
      121 gcgaatccgc tctgggcgcc ggcgccagca acgtgtacct gatcgaggag cccatggccg
      181 ccgccatcgg cgccggcatg ccggtggcgg atgcaaccgg ctccatggtc gtcgacatcg
      241 gcggcggcac caccgaagtc ggcgtgatct ccctgggcgg catggtgtac gccggctcga
      301 tccgcgtcgg cggcgacaag ttcgacgaag ccatcgtcaa ttacatccgc cgcaactacg
      361 gcatgctgat cggcgagacc accgccgaat ccatcaagaa ggaaatcggc tcggccttcc
      421 ccggctccga ggtcaaggag atggaagtca agggccgcaa cctggccgag ggcatccccc
      481 gcgccttcac catctcctcc aacgagatcc tcgaagccct caccgagccc ctcaaccaga
      541 tcgtctcggc ggtcaagatc gccctggaac agacgccgcc cgagctgggc gccgacatcg
      601 ccgagcgcgg catggtcctc accggtggcg gcgccctgct gcgcgacctg gaccgcctgc
      661 tgatggaaga gaccggcctg ccggtgatcg tcgccgaaga cccgctcacc tgcgtggtgc
      721 gcggctcggg catcgccctc gaaaagatgg accagctcgg ttccatcttc accagcgaat
      781 aaaccgcgtc agggcggccg ggacgtacgc ggtgaacgcc tcgttcgacc gtgcgcgtcc
      841 gtcatccggg tttgtcccgg cggtcgtcgt gcactgaccg gacgggcaga ccatggtcgg
      901 ccattcgcct cctcccttct tcaagcgcgg tcccgcgccg ctggccaagc tcgtgttttt
      961 cggcgtgctg tccctggcca tgctggtgtc cgacctcaac ctacgctacc tggacgcctt
     1021 ccgccagggc ctgtcggtgc tcgcctatcc gctgcagatg gccgccgcca cgccggccga
     1081 cttcgtccgc aacgcgtccg actacttcgc ctcgctggtg cgcctgcaac gcgagaacag
     1141 cgaactcaag cgcaagcagc tggccggcgc cgagcgtctg ctgcgccagg accagctcga
     1201 acaggagaac cgccacctgc gcaccctcct cgacgcccgc gagcgccagc cggtgaagtc
     1261 cgtcattgcc gagattctgt acaccgccaa ggatcccttc tcccgcaagg tgatcctcga
     1321 caaaggcgcc caggcgggca tcgaggcggg tcaggcggtg gtggatgacc tgggcgtgat
     1381 cggccagatc acccgcgtct tcccgatgca ggcagagctc accctgctca ccgacaagga
     1441 ccaggcgatc cccgtgatga tcgtgcgcaa cggtctgcgg gcgatgctct tcggtgccgg
     1501 caacggcctc atggagctgc gctatctggc cgccaacgcc gacgtgcagc ccggcgacaa
     1561 ggtggtcacc tccggcctcg acggcgtctt catcgccgga ctgccggtgg ccaccgtggc
     1621 gcgggtcgat cgtgaccagt cccagacctt cgcccgcatc ccctgcctgc cggcggccgg
     1681 cgtggaacag ttcggccacg tgctggtgct cggccaccgg gaaagcgtgg cgccgcgccc
     1741 gcccgaaccc gacccgctgc cgggcaaggc cggcaagggc cgcaaggcgc gcctcgccaa
     1801 ggagtaagcc gtgcagccga ccaaccgttc gagccgaatc ctgttgccgg tgaagatgtg
     1861 gttcgtcttc ctgaccctct tcatcgccct gttcctgaac ttcatcccca ccggccatct
     1921 gccggcggtg ccggatttcg tcgccctggt gctggccttc tggtgcatcc gtgagccgct
     1981 gcgcatcggt atcggcaccg gcttcgtgct gggcgtgctc accgacatcg gcgtcggcgc
     2041 ggcaatgggg cagtacgccc tggcctatgt gctgctggct tacgcggcca acgagctgtc
     2101 gcggcgggtc ttgtggttca cgccggtgga gcaggccctc cacgtcctcc cgctgctgtt
     2161 cctcagccag gtggtgatgg tggtggtgcg ggtcatcgcc ggtgcggact tcccaggctg
     2221 gtggatgttc ttcggcagct tcgtcggcgc cctgctgtgg attcccctgc actacgtgct
     2281 gctgctgccc cagtaccagc ccgtcgagcg ggacgagaac cggccgatct gacggagctc
     2341 cggcgatgcg cgcaggcccc ttcctcccgc tgcatgcggg ccgccatccc gagcggcaac
     2401 gcccatgaag aacgtgcgca cccccgagca ggatctcgcc cgcttccgcg gccgcctcgc
     2461 agtggccggc gtggtggcgc tggtgtgctt cggtctgctg gtggcccgct tcttctacct
     2521 gcaggtggtg cgttacgatt actactccac ccgcgccgaa gacaaccgca tctccctggt
     2581 gcccatcgtg cccaaccggg gcctcatcgt ggatcgcaac ggcaccgtgc tggcgcgcaa
     2641 ctactcggcc tacacgctgg agatcacgcc gtcccgggta gccgacctgg aagccaccat
     2701 cgaggccctc tccaagctgg tggacatcca gcccaaggac cgcaagcgcc tcaagaagct
     2761 gatggacgag tccaagagtt tcgagtcgct gcccatccgc aaccgcctgt ccgacgaaga
     2821 ggtggcccgc ttcatcgccc agcgctaccg ctttcccggc gtcgagctga aggcgcgcct
     2881 gttccgcgac tacccgctgg gcagctttgc gtcgcacatc atcggctaca tcgggcgcat
     2941 caacgaccgc gacctggaac gcatcgagga caacgacgac accgccaact accggggcac
     3001 cgaccacatc ggcaaggccg gcctggagaa gagttacgag cgcgaactgc acggcgtcac
     3061 cggcttcgag gaggttgagg tggattccgg cgggcgggcg atccgctccc tgcgccgcaa
     3121 ccccgccacg ccgggcaaca acctgatcct gaccgtggac ctcaaactcc aggaagtggc
     3181 cgagaaggct ttcggcaacc gccgcggcgc cctggtggcc atcgagcccg ggagcggcgg
     3241 cgtgctggcg ctggtgtcgg tgcccacctt cgatcccaac ctgttcgtgg acggcatcgc
     3301 cccccaggac tgggacgagc tcaacacctc gcccgatcac cccatggtga atcgcgccct
     3361 caacggcgcc tacccgcccg gctccacctt caagcccttc atggccctgg cagccctgat
     3421 gagcggcaag cgcaccgccg cccaggccat cgccgacccg ggcttcttcg acttcggcgg
     3481 ccaccacttc cgcgacgaca agccgggtgg ccatggcacc gtggacatgt accgctccat
     3541 cgtgcagtcc tgcgatacct actactaccg cctggccaac gagatgggca tcgagctgat
     3601 tgccgccacc gtcggccagt ttggcttcgg cgcccgaacc ggcatcgaca tcgagggcga
     3661 atccgtcggc gtgctgccct cacccgaatg gaagaagcgc cgcttcaaga agcccgagca
     3721 gcagaagtgg ttcggcggcg aaaccatctc ggtgggcatc ggtcagggct acaacgccta
     3781 caccatgctc caactcgccc acgccaccgc caccgtggcg gccggcggcg tgatgtacaa
     3841 gccccacctg gtgaagttca tcgagaacgc caaggacggc gaaaaacgcg tcatcgagcc
     3901 ggagccgatc aagaccattc cgatccgccc cgagcacatc gagacgatcc gccgcgccat
     3961 ggtcggcgtg aaccgcgaag gcaccggcgc ccgcgccttt gccggcgcgc cctacgcggc
     4021 cgcaggcaag accggcaccg cccaggtgtt ctccctgaag ggcgccaagt attccgccaa
     4081 gatcgccgag cgcctgcgcg accacgccct cttcatcgcc ttcgccccgg ccgacaagcc
     4141 caccatcgcg ctggccgtgc tggtggagaa cggtggcttc ggcgccgagt ccgccgcccc
     4201 catcgcccgc caggtgctgg actactacct gctcggcaag ctgccagcca gcgtggcgcc
     4261 cgaatatccg ggcgccgaag gcgtgccgga ggagcgcccc gttgagtgaa ttccgcttcc
     4321 agcccctgcg ctggatcgcc cgtctgctgg cgcccctcga cgggccgctg gtgaccatcg
     4381 tcctggcgat cctgtgcttc tcgacgatcg tgatggtcag cgcatcgccg gaacgcctgg
     4441 acaccctggc cctcaacatc gccgtggcct tcacggtgat gtgggtggcg gccagcgtgc
     4501 ccccccagcg catgatgacc atcgccctgc ccctctacgt gctgggcgta ctgctgctta
     4561 tcggcgtcgc cctgttcggc gacgtctcca agggagcgcg acgctggctc aacatcggcg
     4621 tggcgcgcat ccagccctcg gaactgatga agatcgccat gcccctgatg ctcgcctggt
     4681 acttccagag ccgcgaaggc atgatccgcg tgcgcgactt cttcatcgcc gcggccctgc
     4741 tgctggtgcc ggtcggcctc atcgccaagc agcccgacct gggcaccgcc atcctggtgg
     4801 cggcggcggg tttttacgtg ctgttcttcg ccggcctcac ctggcgcctg atcgtaccgg
     4861 tggccgtggt gggcatcgtc agcgtcgccg ccatcgtcgc caccggcgac cggatctgtc
     4921 agcccgacgt accctggccc ggtctgcggg actaccagaa gcaccgcatc tgcacgctgc
     4981 tcgatcccac ctccgacccc ctcggcaagg gcttccacat catccagtcc accatcgcca
     5041 tcggctccgg cggcgtactg ggcaagggct ggggcaaggg cacccagacg cacctggact
     5101 tcctgcccga aagacacacg gattttatct tcgcggtgct caccgaggaa tttggactag
     5161 ccggcgccct gattcttgcg gtactctaca tcctgttgat tttccgcggg cttttcatcg
     5221 ccgcgggtgc cccgaccctg ttctcgcgcc ttctcgccgg cgccctgacg ctgatcttct
     5281 tcacctacgc gttcgtgaac atgggcatgg tgagcggcat tctccccgtc gtgggcgtgc
     5341 cgcttccctt cgtcagttac ggtggcacag ccctcgtcac cctgtgtctc ggtgtgggga
     5401 tgctcatgag catccagcga caccgacgcc gggtgggtac ctgaacaccg cgcagcaccg
     5461 ggccttgcgg ccggctgcgc cctgacagga aacgtgcatc atgacccctg aagccatccg
     5521 ccgcccccgg cgagggccct gctgcgccat cgctccggtg tccgccctgc tgctggcctt
     5581 cggcgccttg ctggccggta cagcccatgc cgccgccttc gaaggcatgt tccccagcga
     5641 gacgctccac atcgccccgc cgcccgatgc ggacgatgcg cccgccgctg ccgcgccggt
     5701 cacctatgcc gcgccgtcct acgccctgcc ggaccggcgc gaactcgccg ccctgtccct
     5761 ttaccgggag cgcggcatcg ccagttacta cggccgcccc ttccatgggc gcaagacctc
     5821 cagcggcgac ctcttcgaca tgtacgcgat gaccgccgcc cacccgacgc tgcccatccc
     5881 cagctacgtg cgggtcacca acctggccaa cggccgctcg gtggtcgtcc gcgtgaatga
     5941 ccgggggccc taccatccgg gacgtgccat cgacctctcc tacactgccg ccttcaagct
     6001 cggctacgcg gaccagggtc aggccaacgt ggagatcgcc caggtcctgc ccgaggaagt
     6061 cgccttcgtc cagacccgcc cggtcccgcc gctgcgccgt gctgccagcc gacctgcgcc
     6121 ggcccaggtc gccgccgcgg ccgtgcccgt gccggcggct gcgccgcctg ccccacgtac
     6181 cgaaagcacg ccggtcgctg cgcccatcgc cccgccggtt gccacgcctg cggtcgctgc
     6241 cgcgcccctg gccgacgctg cgtccagccc ggtctttctg cagctgggga ccttcggaag
     6301 ccttgtaaac gccgaatcct tcaagacctt cgtcgaacac gagctgcgca gcgtgaagga
     6361 aaccgtctcc ctgctcgcca gcgacggcaa gtaccgcctt cacctcggcc cctttcccag
     6421 cgcccaggaa gcacgctcca tggccgagcg catcgccacc accctcaagc tcaaacccag
     6481 cgtcgtccaa cgctgatccc cgtccggacg tgtgatcaaa ttcacacgaa tccagtgacg
     6541 catgccgact cgcgatcccg gccggatcgc taagctatcg gcgtgggctg gaatcccggc
     6601 cgcccgcctc cgaacgcccg ggacaacctc aacccggaag gaggatccca tgcccgtgcg
     6661 tcccgccccc cagcgtccca agtaccgccc cctgcgccgc ccgtcctcgc cgtcggccac
     6721 cctcctcgat ccagacggac tccccgacga aaccgacctg acgcgcatcc gcgtccgcat
     6781 ggccgccgcc cggcagcagc agcagcgggc ccacgtgccc cactgaatcc accgaacccg
     6841 cgctcggatg gcgcgagccc tcttcgggct cctgcccgtc gtgccggggg cacgcccagg
     6901 cgaggcgtgc cggcagtgac ggacggacga cccgccggat cgccccctca tcgcgtttct
     6961 cccctcccgg ccgcgcccgc gcacaaacgc ttccggggac gccccccgcg cccggaacgc
     7021 cagcaccgcc ccctcacaga cagacagcct ccctgccggc ctgcagcccc cgcggggcga
     7081 tcctcctccg cgaggggcca tcacccaggg acgcgtgacc gcccctcgcg cgcgaatgtc
     7141 cgcccctcgc gtccgaggga tcatccctcg cctacgcgtg agcatccctc gccgacgagt
     7201 gaatttcact cgcacgcgac cgtctcccac acgccgacga gcgtggctca ccccgcagcg
     7261 aatgacgctc atcggctgac gagaacactt cagtcgcaca cggacgattt ccacccgacc
     7321 gcgggtgagc gtcaggcctg cgcccccaac cgaccggcca aagtgtgacc aaattcacgc
     7381 aaatcctgtg gcacatgacg tcgaactgca tcggccatct gcgtaatctg ggacccgtga
     7441 tctggaacaa cgggatgccc gccggaccac acccgcccac tcttccactc gcaggagaac
     7501 gccatcatga ccaccgaagc ccgtgaaact gctgtccgca tcggcaccgc cgacatcgaa
     7561 accgtcgccc gcgaaggtgt cgcccgcgcg ctggccgccc gcacccaggc caccgaactg
     7621 agcgccgacg agatcgacgc cgtcagtggc ggcctgaccc tctcgacgat ccagctcgcc
     7681 caaccgaccc tggtcctcaa gccgggtatc ctcgccggcc cgtacccggt ggatgtgctc
     7741 aagggtggcg tcatcgccgc ggccaccgtg cagaagtact gagagaccgc cgccatggcc
     7801 acccagcccg agatcctgtg ccccagcgcc tttcccgaat gggacggggc acgcttgttc
     7861 gcggtggtcg gcggcacacc ggaacggccg gaaacggcct acctcgacca gctccagccg
     7921 gtgaccccgg aactcctgga gctggccggg ccggtggccc cgatcgaagt cttccgtttt
     7981 gccgccccct gcgccgagcg cgcctgccag cagttcgacg gctccaccgg ccgctgtcag
     8041 ctggcccgca agacagcccg gatcgcaccg gtggtggtgc acaagctgcc ccgctgcggc
     8101 atccgcgcca actgccgctg gtggctgcag gaaggcccct ctgcatgcaa acgctgcccc
     8161 caggtcgcca cgctcaacta cgcgccgagc gaggccatcc gccatgcagc cgatcctgcc
     8221 cgccccctcg acgaagttgg caccaccgca ccgcttggca cctgaaccca cccctttcca
     8281 ggagaccatc atgagcagca cccgcaaagc cctctgcgtc ggcatcaacc agttcaagaa
     8341 ctacccctcc gcggcccttc agggctgcgt caatgacgcc cgcgacatgg cgtccctgct
     8401 tcagcaactg ctcggcttca ccgcggccga catcgccgtg ctgaccgacg cccaggccac
     8461 caaggccgcc atcatggccc agctcaaagc catggtggac ggtgccaagg ccggcaagta
     8521 cagctacctg gtgttcagtc tctccagtca tggcacccag gttcccgacc tgagtggcga
     8581 tgaacccgac cgcgccgacg aggccttctg cccccacgat ctggcccaga agggcaacca
     8641 gtgggacccg gcccacatca tcaccgacga cgaactccac gacctcttcg tgcagatccc
     8701 acccaccgtg ctgctggagg tctatctgga cacctgccac agcggcaccg gcctcaaggg
     8761 gatcgacatg ctcctcgacc gcaagccgcg ctacctgccg ccgccgtccc tggaagcctt
     8821 tcgccagatc gaaggacgtc gcagccgggg gatcgcccag gccatcaagg aaaagggccc
     8881 ggccacccac aacatcctgt gggcggcctg ccgtcccgac cagaccagcg ccgacgccca
     8941 cattgccggc ggctggcatg gcgccttcac ctatttcttc accaaggaga tgcgcgccag
     9001 caagaacaag ctcagccgcg cccagatcct gaccaaggtg cgggccgatc tggcggccgg
     9061 tggctatacc caggtcccgc agctggaagt ggacgccacc acgcggcaac tggccgcggc
     9121 ctgacatcgg cgcagtccag cgtcgcccgc cgtccggccc acgcgggcga cgcctcaccc
     9181 tcagggctca ttccatccag gagttccatc atgcgtaccc ctccctgtcc gtccgcccct
     9241 gtcgaactgt ccgccgctga gattgaagcc gtatccggcg gcatgggcgc acttgccatc
     9301 ctcgcgggtg ccgcggcgtt cgtcgcagcc ggggcccttg ttgcggcggt cgccgtcacc
     9361 gctgacaacg ccgccaacgg ccccctcgcg ggcgcctggt accgcttcac caagtaagtc
     9421 cccgcggccg gggcgtcccc cacgttccgg ccgcgtcctc ctgcctgcta ccggagacgg
     9481 ccatgcgccc tgctctgttc cgccccgaag tcctggccca gcgcccggac ggccgccacg
     9541 gcgaggtgct gatcgcccat ccgccggccc tgatcctgct ggtcgcgctg gccgtggcct
     9601 tcaccgcagg cctcgccgcc tacgcgtggt ggggcgagta cacccgcaag gaacacgtca
     9661 gcggcagcct cgtgcccacc cgtggtctga tccgtgtctt caccccccag gccggcacgg
     9721 tgctggaaaa ggccgtgcgc gagggccagc ctgtccggca gggcgacgtc ctgctggtcc
     9781 tgagcagcga acgggcaagc accgcgaccc gcgagaccca ggccgcccag ctcgccgccc
     9841 tgcgccagcg ccgcgacgcc ctgcgcgccg agcaggccaa gcagaccgag atcgacaccc
     9901 tgagcggcag cgccgcagcc gaccgcgccc acggcctggt gaaggaactt gccgaggccc
     9961 gcgcccagct cgatctgcaa cgcagccggg tggccgccgc cgagcgcacg gtggcgcgtc
    10021 acgagcagct ggtcgcggcc cgcttcgtcg ctgaagccac gctccagcag aagcaggaag
    10081 agctcctcga ccaacgcgcc cgccaggccg agacccagcg catcatcgcc ggactggaac
    10141 gggatctcgc cacggcgcgg gccgagttgg ccagcgccgg cctccggcgc gccaacaccg
    10201 cctcggccat cacccgccag atctcggagc tggaccagca gctcaccgag accgacagcc
    10261 ggcgcagcgt ggtcatcacc gcgccagccg acggcatagt caccacgctc ctcgccgaac
    10321 ccggccagac cgccacgccg ggtacgccgc tgctgtccct gctgcccgcg ggtgccgaac
    10381 tgcaggcaca gctgctggtg ccgacccgtg ccgccggctt catccgcacc gggcaggagg
    10441 tggtgctgcg ctaccaggcc tttccctacc agcgcttcgg ccatcacctg gggcaggtgg
    10501 tggaggtcgg ccgcagcgtc atccagcccg ccgacgccgg tcaggccggc agcgtgcagg
    10561 agccggtcta tcgtgtcacc gtacgtctgc cggcccagag cgtgcaggcc tacggcgagg
    10621 cgatggccct gcaggcgggc atgaccctgg atgccgacgt acgtctcgac cggcggcgcc
    10681 tgatcgagtg gctgttcgac cccctgctgt ccgtcaccgg ccgtatctga ggagatcccc
    10741 gccatgctcg acttcggatt tgcgtcgagg acgcccctga tcctgcaggg ggaatccgcc
    10801 gaatgcggac tcgcctgcct tgccatggtg gccggccatc acggccaccg cattgacctc
    10861 gcttccctgc gcgcgcgcca cagcctgtcc cagcggggcg tcaccctggc cgacctgatg
    10921 cgcatcgccg gcaccctgca tctggcccca cggcccctgc gcctggagcc ggaacagctc
    10981 ggacagctgc gtctgccctg tgtgctgcac tgggacctca accattttgt cgtgctgatc
    11041 cgcctgcggg gcgaccgggc gagcgtccac gatcccgcct gcggggcgcg cacgctgcgc
    11101 ctggccgagc tttcccgcca cttcaccggc gtcgccctgg aactcaaccc cacggacgcc
    11161 ttcacgcccg ccacggaacg ccgccgcgtc cgcctcggcc aactggtcgg ccccctgcgc
    11221 ggattgggcg gcagcgtcct ccaagccctc gtcctcgccc tcgtgctgca actcttcgcc
    11281 ctgctggccc ccttctacat gcagtgggtg gtggatcagg ccctggtcac tcaggaccgg
    11341 gacctggtca ccgtgctggg cgtaggcttc ctcctgctgg cgctggtcaa tgccggcgtg
    11401 gccgccctgc gcggctgggt gctgatggtg cttggcacct ccctcaatct ggccatgctc
    11461 acgcgcctgt tccggcatct gatccgcctg cccatgacgt ggttcgacaa gcgccacctg
    11521 ggtgacgtcg tgtcgcgctt cgaatccctc aacagcattc aacgcaccct caccggcggc
    11581 ttcctcgaag ccctggtgga tggcgccatg gcgctgctca ccctggccat gatgctcttc
    11641 tacagcctgc ccctggccag cgtggcaatc gtcgcggcag cggcctacgc cgggctgcgg
    11701 gcggccttgt accagcccca gcgacgtgcc gccgaggcgc acatcgtgcg cggagcccgc
    11761 cagcacagcc acttcctgga gacggtgcgc ggcatccaga ccctccgcct gcacgcccac
    11821 gaggaacccc gcacggtggc ctggcacaat ctggcggtgg accagttcaa tgccggcatc
    11881 cgcagccagc gcctgggcct gctctaccag gggctcaaca cgctcctgtt cggtatcgag
    11941 aacgtggtca ccgtgtggct gggcgcccgc ctggtgctcg acaccgccag cggcgcgggc
    12001 ttttcggtag gcatgctgtt cgctttcatc gcctacaaga accagttcgt gcagcgcatg
    12061 gcggccttca tcgaaaaggc cctggagctg cgcatgctcg gcctgcacgc cgaacgggtc
    12121 ggcgacatcg ccctcgccgc ggccgagccc gacgagccgc cggcatcggc agcggctgcg
    12181 cccctcgcgg gccatctgag cgtgcgcaac ctggcgttcc gttatggcga caccgatccg
    12241 cgggtgctgg acgacgtgag cttcgacgtg cgcgccggcg aatcggtcgc gctggtcggc
    12301 ccttccggcg gcggcaagac cacgctggtg aagctgctcc ttggtctgat ggcgcccgag
    12361 tcaggcggga tcgaggtgga tggcgtgccg ctggcccgct tcggcctgcg ccgttaccgg
    12421 gaacaggtcg ccagcgtgat gcaggacgac cagctcttcg ccggctccct cgccgacaat
    12481 atcgccttct tcgaccctca ggccgacccc acccgcatcg aggcctgtgc acgcctcgcc
    12541 gccgttcacg acgacatcgc agcgatgccc atggggtatc acacgctggt cggcgacatg
    12601 ggcacggtgc tctccggtgg ccagaagcag cgcgtgctcc tcgcccgcgc cctctaccgc
    12661 cgtccgcgca tcctggtcct cgacgaagcc accagccacc tggacatcgc ccgcgaacgc
    12721 tgcgtcaacg acgccatccg cggcctcccc ctcacccgcc tcatcgtcgc ccaccgcccc
    12781 gagaccatcg ccagctgcga ccgggtgatc gtgctgacgg gcgggcgggt gacgaacgag
    12841 atgtatgcag tggcaggggg gatggggggt gcgg
//
LOCUS       DAOQMM010000106        12540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2187_length_12540_cov_43.101161, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000106 DAOQMM010000000
VERSION     DAOQMM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2187_length_12540_cov_43.101161"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="PLH85_14360"
     CDS             <1..1749
                     /locus_tag="PLH85_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB65797.1"
                     /translation="AVVAAPPVYADPATEAIQTPPSGTWPSFGRDYSNQRFSPLTQID
                     AGNVHRLARAWTYRSGIQATFQATPIVVDRTMYLSLPFNHVVALDARTGEERWRYVHT
                     LMPGRTPCCGPSNRGVAVADGKVFMGTVDGRLIALDQRSGARLWDVDLLERTPTQTES
                     VSRIAPDDPLAAARFVGSTGAGIAMAPVVYQGKVLIGITGVGFGLHLDSSAENPLTVV
                     GLPGNYGRSGFLAAFDAATGARLWTFDTVRADAWEGDYRSHTPDGHALPRDIPGEKAA
                     AARYPSAWKFGGGAAWSSPAIDTLRGLLYFGTGNPSPNMEDSSRPGDNLYTTSLVALE
                     IETGALRWHFQQVPHDIWGYDVASPAVLFDFERDGRRIPAVAQASKLGWLYIHDRETG
                     ALLLKSDAFVPQHNLFQRPTTAGLRVHPGVTGGVNWSPMALDATAGLAYVAAMHWPVI
                     YTLHEKPAANGRPASRYTSMDPVKDERWGVLAAIDLKTGHVRWQHRTREPLIGGTLAT
                     AGNLVFVGEGTGELDAFDATRGTLLWQHTHDAGVNAPPITYAIDGVQYVAVVVGGNHL
                     FGFTQGDQVAVYALGR"
     gene            complement(1771..2319)
                     /locus_tag="PLH85_14365"
     CDS             complement(1771..2319)
                     /locus_tag="PLH85_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB65798.1"
                     /translation="MPSTAPGLLTPRLHLRPWRAEDLSPFAALNADPEVMRHFPATLE
                     RAASDALARRIDQLIAAQGWGFWAAELRASGAFIGFIGLHRPTHLPFSPCIEVGWRLA
                     RPFWGLGLATEGARAALEFAFHTLAADEVVAFTALSNQRSEAVMRRLGMQRDAAPFAH
                     PDLPDGHPLQAHVLYRIRRPGA"
     gene            complement(2350..3000)
                     /locus_tag="PLH85_14370"
     CDS             complement(2350..3000)
                     /locus_tag="PLH85_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65799.1"
                     /translation="MLKFAAALAGGLALLLLPRLGRLRTRYDTAALHPLPGDAPADDP
                     ALVQRLTAWARDGMGCGATVLPTAQPRVPRPLAVAWADAAAADSVQHAAYRLAGYHQL
                     DTRSRLGGLLYRVGVQLRPLLWCVPLAADAPWDDAWFAAGSTLPLDALRAWRPRRPTV
                     IVVADATQVTALVDALTGTPSHGGHALRVVGLLPAGAVPPALPGDVPVCDLHATPR"
     gene            complement(2994..3851)
                     /locus_tag="PLH85_14375"
     CDS             complement(2994..3851)
                     /locus_tag="PLH85_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB65800.1"
                     /translation="MAKRKPAAGTPAPVAHPDLRPGQSVELLQALHILTRDGQLNQDS
                     RRKLKQVYHLHQFIEPLLAEVLAERGDLSLADHGAGKSYLGFILYDLFLKQQAAGTVY
                     GIETRRELVEHSRALAERLGFARMHFLDVSVADSTHAAELPARIDVVTALHACNTATD
                     DAIQFALAKDARFLVLVPCCQAEVAAVLRQNKNASFGLTPLSEIWRHPLHTREFGSQL
                     TNALRCLQLEAHGYQVTVTELVGWEHSLKNELIIARRTGQPRRNAMARIEQILDQLNL
                     TELRSRFAC"
     gene            complement(3838..4401)
                     /locus_tag="PLH85_14380"
     CDS             complement(3838..4401)
                     /locus_tag="PLH85_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HNB65801.1"
                     /translation="MNHEEVIAATRAWIEKAVIGLNLCPFAKAVYVKDQVRIVVSDAR
                     HIDGFLEDLDRELDHLAAVDPEVTDTTLLVHPTLFPDFLDFNDLTQIADEAVSEHELD
                     GVLQIASFHPDFQFEGTEADDITNYTNRSPYPTLHLIREASIDRAVEAFPEAEMIYER
                     NMETLRTLGIAGWKALGLSTPKRDGEA"
     gene            4484..5404
                     /gene="ylqF"
                     /locus_tag="PLH85_14385"
     CDS             4484..5404
                     /gene="ylqF"
                     /locus_tag="PLH85_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNB65802.1"
                     /translation="MSIQWFPGHMTSARKKAAESMERIDVVIEVVDARLPEASTNPMI
                     RELRAHRQRPCLKILNKADLADPAVTARWIAWYNAQKGVSAVALSCKKPGDVAKVVGL
                     CRTLAPHRNDRLKPLRMMIMGIPNVGKSTLMNALLKKRVAKVGDEPAVTKSQQAIDLS
                     PTASLVDTPGMMWPKILHDSDGFMLAASHAIGRNAVIDEEVANFLGGLLLQRYPALLV
                     KRYGFPVDGLDGPALVEAIGRRRGCLQKGGGLDLEKASLILLQDYRDGALGRVSLETP
                     ETRAVMLQAYAEALAQKAQAAEDRPEDSPE"
     gene            5522..7723
                     /locus_tag="PLH85_14390"
     CDS             5522..7723
                     /locus_tag="PLH85_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65803.1"
                     /translation="MTRPNNAALTRKKIQLGFLCGACLVAALVSGWLWRSWQDARTYE
                     FSHLSTIAELTGNSLDQYFSRHEHQLRVLGRDILREAAEPDAFPALLARFKESEPDCL
                     FLTLSAADGTLIATDFTGPRETLPNTQDLPGRRAAYEELARQDRLNIGRPFEGRLMKA
                     WIMPLRLGVRDDTGALRYVISCTLPLSRQQTFWQNLSLPPDSSFGLLRDDAYLVSRYP
                     PAPDGNLQDTYGKPRDGALVMHLREAGFPHRGIVYGYNSVLRKDTLFAYQRLGHYPLT
                     VFVTTPGDGALNRWLDQLYLPSFLALISLVVSVLMYRTIARRIAVSEELERYQGRLEE
                     LVEQRTHELADAKSAAETASQAKSQFLANMSHEFRTPLNGLMGMLRLARGRMRDPAGL
                     EQLDKAAIAATHLATIINDVLDVSKIEAAQLQLEAIEFFLPPVVDTAFSMNTARAAAK
                     GVSLRSELTPAVAAQAYVGDPLRLGQILINLVSNAVKFTERGSVTVLARRLDHTDSRA
                     TLRFEVIDTGIGIPRDQQDRLFSAFEQADSSTTRKYGGTGLGLAICKRLVELMGGRIG
                     LDSEIGRGCTVWFEITLPVTAGRPAADAAPDLDALEAEIRARHAGKIVLVVEDEPINQ
                     EITRCNLEDIGLVVQVAGNGQEAVAMAGQTDYALILMDMQMPVMNGLDATQAIRRGAR
                     NARTPILAMTANAFVEDRDLCLAAGMNGHIAKPVELAVLYREVLAWLGSDA"
     gene            7959..9389
                     /locus_tag="PLH85_14395"
     CDS             7959..9389
                     /locus_tag="PLH85_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNB65804.1"
                     /translation="MAPVPKLIVLLLGGILAGCAVPRVGPDYQTPQAELPAVWTAQLP
                     DASANAPDAAALARWWTQFDDPALDRLVGEALSQGLDVRLAQARVQQARASRQLAEAG
                     LSPTVAASAGGTRSKSSQAVAAVPTRTLYDAGFDASWELDLFGGTRRGIEAAQADVAA
                     SQASLEQVRVTLVAEVARNYVDLRAAQQRIRIARDNLASQRETLQITEWRYQAGLARA
                     SEVEQARTAEAQTRAGIPDLEIDLVAAQNRLAVLLGRAPGSLHDQLAAVKPLPALPTQ
                     LATGIPADVLRQRPDIVAAERTLAAETARVGQKLAQRFPSLSLSASFGWQAYSTAALG
                     GAANAARALGATLATTLFDGGRLRSQVDVQSAVQEQALVAYQSTVLTALEEVENALKS
                     HAAARERFAARQAAAEAARNAADLSRKLYQAGLADFQQVLETQRTQLSAEDSLATADA
                     SLRTSLISLYKALGGGWDAAARNGTS"
     gene            9386..10660
                     /locus_tag="PLH85_14400"
     CDS             9386..10660
                     /locus_tag="PLH85_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB65805.1"
                     /translation="MNDTSSSDSLKQLLDQAPRGGRGVAWRRWLWAGGLVLVLGGVFV
                     ALRGDKGAGGHYLTEAAERGALVVTVSATGTLQPTTSVDVGSEQSGTLAEVLVQENDR
                     VKQGQLLARLDLAKLQDAVSKSRAALAAAEASVAQARATVAESRASLARMRQVAELSG
                     GKVPSKTELETAEATLERAVANEASARATVMQAQAALKTDETNLSKGTIRSPVNGVVL
                     SRKVEPGQSVVAAMTIPVLFTLAEDLTAMELQVKVDEADVSSVRLGQPASFTVAAWPA
                     RTFPATIERVGIGSTITDNVVTYKTVLAVKNDDLALRPGMTATASIVTAKRDDALLVP
                     NAALRFTPPARGADARQGSFVSSLLPRPPSETKHSTAAPAGSPQVWVLGDDGPRAVPV
                     KTGVSNGRQTEILGGELKPGMAVITEYQEGRP"
     gene            10657..11373
                     /locus_tag="PLH85_14405"
     CDS             10657..11373
                     /locus_tag="PLH85_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB65806.1"
                     /translation="MSAERAEPLIRLVGVTKTYGEGGVAYQALRGIDLEVAAGEFVAI
                     MGPSGSGKSTVMNILGCLDTPTSGAYLFRGIHVEQLDRNQRALLRRHSLGFVFQGFNL
                     LARTSAQENVELPLLYRGETAPARHAAARAALDRVGLAGWEPHTPAELSGGQQQRVAI
                     ARAIVTQPDVLLADEPTGNLDSQRSHEIMALLVELNRKQGITVLMVTHEPDMAAYARR
                     IVTFVDGRVARDEAVPERAL"
     gene            11370..>12540
                     /locus_tag="PLH85_14410"
     CDS             11370..>12540
                     /locus_tag="PLH85_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB65807.1"
                     /translation="MSSVLVSAFLLALREIRRNLMRSFLTVLGIVIGVAAVVTMVTLG
                     NGATRAVSDQISSLGSNLLMVRPGQRLGPGRDSAGAPAFKRADAEALRTQLTGMDEVV
                     PVAGSSVTVVAGNNNWSTTVSGTTNGYLKAGNWKIASGRAFQDDEERAGKSVCVIGAT
                     VRRELFGTRNPLGDEIRVKTFACQVVGLLAAKGQGAMGMDQDDTVLMPLATVQRRLTG
                     TQDVSTLMVSLHDGVDAKPVMAQIRALLRERRKLADNEADNFNVMDTKQIAETLSGTI
                     RTMTGLLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGIRLAIGALEHEVLLQFLIEA
                     VVLSACGGLIGILLALLACVALSALLSMPFLFNTGINLLAFLFSALIGVVFGYMPA"
BASE COUNT         1926 a         4557 c         4254 g         1803 t
ORIGIN      
        1 gcagttgtcg ccgcaccccc cgtgtacgcc gaccccgcca ccgaggccat ccagacgccc
       61 ccgtccggca cctggccgag cttcgggcgg gattacagca accagcgctt ctcgcccttg
      121 acgcagatcg acgccggcaa cgtgcatcgg ctcgcgcgcg catggaccta ccggagcggc
      181 atccaggcca cctttcaggc gacgcccatc gtggtggacc ggaccatgta cctgtccctg
      241 ccgttcaacc acgtggtggc cctggatgcg cgcaccggcg aagagcgctg gcgttacgtg
      301 cacaccctca tgcctggcag gacgccgtgc tgcgggccgt cgaaccgggg cgtggcggtg
      361 gctgacggca aggtcttcat gggcaccgtg gacggtcgcc tgattgccct cgatcagcgc
      421 agcggtgcca ggctgtggga cgtggacttg ctggaaagga cgccgaccca gaccgaaagc
      481 gtgtcgagga tcgcgccgga cgacccgctg gcggcggcgc gcttcgtcgg ctccaccggc
      541 gcgggcatcg ccatggcccc ggtggtctac cagggcaagg tgctgatcgg catcaccggt
      601 gtcggcttcg gcctgcacct ggattcgagc gccgaaaacc ccctgacggt ggtcggtctg
      661 cccggtaact acgggcgctc gggctttctc gccgctttcg acgccgccac cggggcgcgc
      721 ctgtggacct tcgacacggt gcgcgccgac gcctgggaag gggactatcg cagccatacg
      781 ccggacggcc acgcgctgcc gcgggacatc cccggcgaaa aggccgccgc ggcccgatac
      841 ccgtcggcct ggaaattcgg cggcggcgcg gcctggtcct cacccgccat cgacaccctg
      901 cgtggcctgc tgtatttcgg caccggcaat ccctcgccca acatggagga cagctcccgc
      961 cccggcgaca acctctacac cacctcgctg gtggccctgg agatcgagac cggagccctg
     1021 cgctggcatt tccagcaggt gccccacgac atctggggct acgacgtggc cagcccggcg
     1081 gtgctcttcg acttcgagcg ggacggccgg cgcatccccg cggtggccca ggccagcaag
     1141 ctgggctggc tctacatcca cgaccgggag accggcgcgc tgctgctcaa gtccgatgcc
     1201 ttcgtgcccc agcacaacct gttccagcgc cccacgacgg cgggcctgcg cgtccatccg
     1261 ggcgtgaccg gcggggtgaa ctggtcgccc atggccctcg acgcgacggc cggcttggcc
     1321 tacgtggccg ccatgcactg gccggtgatc tacaccctcc acgaaaagcc tgccgccaac
     1381 ggacgtcccg cctcacgcta cacctcgatg gatccggtca aggacgagcg ctggggcgtg
     1441 ctcgccgcca tcgacctgaa gaccggccac gtgcgctggc agcatcgcac ccgggaaccc
     1501 ctcatcggcg gcaccctggc gacggccggc aacctggtgt tcgtcggcga aggcaccggc
     1561 gagctggacg ccttcgatgc cacccgcggc accctgctgt ggcagcacac ccacgacgcc
     1621 ggcgtcaacg cgccgcccat cacctacgcc atcgacggcg tgcagtacgt ggccgtggtg
     1681 gtcggcggca atcacctctt cggattcacc cagggcgatc aggtggcggt gtacgccctg
     1741 ggtcggtagc gccgcccgcg ccgccaccct tcacgcaccc ggtcggcgaa tccggtagag
     1801 gacgtgcgcc tgcagcggat gcccgtccgg caggtccggg tgggcgaagg gcgcggcgtc
     1861 gcgctgcatg cccaggcgtc gcatcaccgc ttcggagcgc tggttgctca gggccgtgaa
     1921 ggccaccacc tcgtccgccg ccagcgtgtg gaaggcaaat tccagcgcgg cgcgggcgcc
     1981 ttccgtggcc agaccgagcc cccagaaagg ccgcgccaga cgccagccca cctcgatgca
     2041 gggggaaaag ggcaggtgcg tcgggcggtg gagcccgatg aaaccgatga acgctccgct
     2101 cgcccgcagc tccgcggccc agaagcccca accctgggcg gcgatcaact gatcgatgcg
     2161 ccgcgccagg gcgtcgctgg cggcccgctc cagcgtggct gggaagtgcc gcatcacctc
     2221 cgggtcggcg ttgagggcgg cgaatggtga cagatcctcc gcccgccagg ggcgcagatg
     2281 caggcggggc gtgagcagtc cgggcgcggt ggaaggcatg gagcgcaggc cggtggatcg
     2341 cgggccgagt cagcgtggtg tcgcgtgcag gtcgcagacc ggcacgtcgc cgggcagggc
     2401 gggcggcacg gcgccggcgg gcagcagtcc gaccacgcgc agggcgtggc cgccgtggga
     2461 cggggtgccg gtcagggcgt ccaccagcgc cgtcacctgc gtggcatcgg cgacgacgat
     2521 cacggtggga cgacgcggcc gccaggcgcg cagggcgtcg aggggcagcg ttgatccggc
     2581 ggcgaaccag gcgtcgtccc agggcgcgtc ggccgccagg ggcacgcacc acagcagggg
     2641 gcggagctgc acgcccacgc gatacagcaa accgcccagg cggctgcggg tgtcgagctg
     2701 gtgatagccg gcgagacggt aggccgcgtg ctgcacgctg tccgccgcgg cggcatcggc
     2761 ccaggccacc gcgaggggac gcggcacgcg gggctgcgcg gtgggcagca cggttgcgcc
     2821 gcagcccatg ccgtcccgcg cccaggccgt caggcgctgc acgagggccg ggtcgtcggc
     2881 cggggcgtcg ccgggcaggg gatgaagggc cgccgtgtcg taacgggtgc gcaggcggcc
     2941 gaggcggggc agcaggagca gggcaaggcc gcccgccagt gccgcggcaa acttcaacat
     3001 gcgaagcggc tccgcagctc ggtgagattg agctggtcga ggatctgctc gatgcgcgcc
     3061 atcgcgttgc ggcggggctg gccggtgcgg cgggcgatga tcagctcgtt cttgagggag
     3121 tgttcccagc ccaccagctc ggtcaccgtc acctggtagc cgtgggcttc cagttgcagg
     3181 cagcgcaggg cgttggtgag ctggctgccg aattcgcggg tgtgcagggg atggcgccag
     3241 atctccgaga ggggcgtgag gccgaagctg gcgttcttgt tctggcgcag cacggcggcg
     3301 acttccgcct ggcagcaggg caccagcacc aggaagcggg cgtccttggc cagggcgaac
     3361 tggatcgcgt cgtcggtggc ggtattgcag gcgtgcaggg cggtgaccac gtcgatgcgc
     3421 gccgggagtt cggcggcgtg ggtggaatcg gccacgctca cgtccaggaa gtgcatgcgc
     3481 gcaaagccga ggcgctccgc cagggcgcgg gagtgttcca ccagctcgcg gcgggtctcg
     3541 atgccgtaca ccgtgccggc cgcctgctgc ttgaggaaca ggtcgtaaag gatgaagccc
     3601 aggtaggact tgccggcgcc gtggtcggcc agactcaggt cgccgcgctc ggccagcacc
     3661 tcggccagca gcggctcgat gaactggtgc aggtgataga cctgtttgag cttgcgccgg
     3721 ctgtcctggt tgagctggcc gtcgcgggtg aggatgtgga gggcctggag cagctcgacg
     3781 gactgccccg gacgcaggtc cggatgggcg acgggggccg gcgtgccggc agcgggctta
     3841 cgcttcgcca tcgcgcttgg gcgtggacag gcccagggcc ttccagccgg cgatgcccag
     3901 cgtgcgcagg gtctccatgt tgcgctcgta gatcatctcc gcctccggaa aggcttccac
     3961 ggcccggtcg atgctcgctt cgcggatcag gtgcagggtg gggtagggcg agcggttggt
     4021 gtaattggtg atgtcgtccg cctcggtgcc ctcgaactgg aaatccgggt ggaagctggc
     4081 gatctgcagg acgccgtcca gttcgtgctc gctgaccgcc tcgtcggcga tctgggtgag
     4141 gtcgttgaag tccaggaaat ccgggaacaa cgtgggatgc accagcagcg tggtgtccgt
     4201 cacctccgga tcgaccgccg ccaggtggtc cagctcccgg tccaggtctt ccagaaagcc
     4261 gtcgatgtgg cgagcgtcgc ttaccacgat gcggacctgg tccttcacat agaccgcttt
     4321 ggcgaagggg cacaggttga ggccgatgac ggccttctcg atccacgccc gcgtggcggc
     4381 gatgacttct tcgtgattca tggctggggt ttccggctga ttcctgcgag ggccgccatt
     4441 ttgccctgat aatgtcgccc tttcattgat tcagcgcacg cccatgtcca tccagtggtt
     4501 ccccggtcac atgacctccg cccgcaagaa ggccgcggag agcatggaac gcatcgacgt
     4561 ggtcatcgag gtcgtggacg cccgcctgcc ggaggccagc accaacccca tgatccgcga
     4621 actgcgcgcc caccgccagc gcccctgcct gaagatcctc aacaaggccg acctggccga
     4681 cccggccgtc accgcccgct ggatcgcctg gtacaacgcc cagaagggcg tctccgcggt
     4741 ggccctgtcg tgcaagaagc ccggcgacgt ggccaaggtg gtgggcctgt gccggacatt
     4801 ggcgccgcat cgcaacgacc gcctcaagcc cctgcgcatg atgatcatgg gcatccccaa
     4861 cgtgggcaaa tccacgctga tgaacgccct gctcaagaag cgcgtcgcca aggtgggcga
     4921 cgagcccgcc gtcaccaaga gccagcaggc catcgacctg tcgcccaccg cgtcgttggt
     4981 ggatacgccg ggcatgatgt ggcccaagat cctccacgat tccgacggct tcatgctcgc
     5041 cgccagccac gccatcggcc gcaatgccgt catcgacgaa gaggtcgcca acttcctcgg
     5101 cggcctgctg ctgcaacgct acccggcgct gctggtcaaa cgttatgggt tccccgtgga
     5161 cggcctggac ggcccggccc tcgtcgaagc catcggccgc cgccgcggct gtctgcagaa
     5221 gggcggcggg ctcgacctgg agaaggcctc cctgatcctg ctgcaggatt accgcgacgg
     5281 cgccctcggg cgcgtcagcc tggagacccc ggagacccgc gccgtcatgc tccaggccta
     5341 tgccgaggcg ctggcgcaga aagctcaagc ggcggaagat cgccccgaag attccccgga
     5401 gtagaccccg gccgcgcgcg cgagccggca gtctgccgcg gatctttcgc cgttcctccg
     5461 acgggacgcc gccgtctctc catctccgca ggggtttcga tcccggaagg catgccgcgc
     5521 catgacccgt cccaacaacg ccgccctcac ccgcaagaag atccagctcg gcttcctgtg
     5581 cggcgcctgc ctggtggcag ccctggtctc gggctggctg tggcgctcgt ggcaggacgc
     5641 gcggacctac gagttctccc atctctccac catcgccgag ctcaccggca attccctcga
     5701 ccagtacttc tcgcgccacg agcaccagtt gcgtgtgctg gggcgcgaca tcctgcgcga
     5761 ggcggccgaa cccgacgcct tcccggccct gctggcgcgc ttcaaggaga gcgagcccga
     5821 ctgcctcttc ctcaccctct ccgcggctga cggcaccctg attgccaccg atttcaccgg
     5881 accccgcgag accctcccca atacccagga tctgcccgga cgccgtgccg cgtacgagga
     5941 actggcccgt caggaccgcc tgaacatcgg ccggcccttt gaagggcgcc tcatgaaggc
     6001 gtggatcatg ccgctgcggc tgggtgtgcg cgacgacacc ggcgcactgc gctacgtcat
     6061 cagctgcacc ctgccgctga gccgtcagca gaccttctgg cagaacctca gcctgccgcc
     6121 ggactcgtcc tttggcttgc tgcgggacga cgcctatctc gtcagccgct atccgccggc
     6181 ccccgacggc aacctgcagg acacctacgg caagccccgc gacggtgcgc tggtcatgca
     6241 cctgcgggag gccggcttcc cccaccgggg catcgtctat ggctacaaca gcgtcctccg
     6301 caaggacacg ctgttcgcct accagcgcct gggccactac cccctcaccg tgttcgtcac
     6361 cacgccgggc gacggcgccc tcaaccgctg gctggaccag ctctacctgc ccagcttcct
     6421 ggccctcatc tccctcgtcg tctcggtgct catgtatcgc accatcgccc ggcgcatcgc
     6481 cgtctcggaa gagctggagc gttaccaggg ccgcctggaa gagctggtcg agcagcgcac
     6541 ccacgaactc gccgacgcca aatccgccgc cgagaccgcg agccaggcca agagccagtt
     6601 cctcgccaac atgagccacg aattccgcac gcccctcaac ggcctgatgg gcatgctgcg
     6661 cctcgcccgc ggccgcatgc gcgaccccgc cggcctcgaa cagctcgaca aggccgccat
     6721 cgccgccacc cacctggcca ccatcatcaa cgacgtcctc gacgtctcca agatcgaagc
     6781 cgcccagctc cagctcgaag ccatcgagtt cttcctgcct ccggtggtgg acaccgcctt
     6841 cagcatgaac accgcgcggg ccgccgccaa gggcgtgagc ctgcgcagcg agctcacacc
     6901 tgccgtcgcc gcccaggcct acgtgggcga tcccctgcgc ctcgggcaga tcctcatcaa
     6961 cctcgtctcc aacgccgtca aattcacgga gcgaggctcg gtgacggtcc tggcgcggcg
     7021 gctggaccac accgacagcc gcgcgacgct gcgtttcgag gtcatcgaca ccggcatcgg
     7081 cattccccgc gaccagcagg accgcctctt cagcgccttt gagcaggccg acagctccac
     7141 gacccgcaag tacggcggca ccggcctcgg tctcgccatc tgcaagcgcc tcgtcgagtt
     7201 gatgggtggc cgcatcggtc tggacagtga aatcggccgg ggctgcaccg tgtggttcga
     7261 gatcaccctc cctgtcaccg ccggcaggcc cgcggcggac gccgcccccg acctggacgc
     7321 cctcgaagcc gaaatccgtg cccgtcacgc cggcaagatc gtgctggtcg tcgaggacga
     7381 acccatcaac caggaaatca cccgctgcaa tctggaagac atcggcctcg tcgtccaggt
     7441 cgccggcaac ggccaggaag ccgtcgccat ggccgggcag accgactacg ccctgatcct
     7501 gatggacatg cagatgcccg tcatgaacgg cctcgacgcc acccaggcga tccgccgcgg
     7561 cgcccgcaac gcccgcaccc ccatcctcgc catgaccgcc aacgccttcg tcgaagaccg
     7621 cgacctgtgc ctcgccgcgg gcatgaacgg gcacatcgcc aagccggtgg agctggcggt
     7681 gctgtatcgc gaggtgctgg cgtggctggg ttcggacgcg tgagggcggg gcgtcgcggg
     7741 cggccgtccg ttcccgcggg gtgttccgga tcgaaaggct gagtgtgccg gcgtctcggc
     7801 gcgtcgcgtg cgtcgcccga gcctgcatgt ccgcgctttc cagtccgtcg tcgcgcgcca
     7861 tgtccggttc gaatccccgc cgtaaagttg ggtaaaagtc ccgcccccgc agaccccgcc
     7921 ccctagaatc gccggcgtgt gctgaatcgc tggtcttcat ggctcccgtt ccaaaattga
     7981 tcgtgctcct gctgggggga atcctggcgg gctgtgccgt gcccagggtg gggccggact
     8041 atcagacgcc gcaggcagaa ctgcccgccg tgtggaccgc gcagcttccg gacgcgtctg
     8101 cgaacgcccc tgacgccgcc gccctggcgc gctggtggac ccagttcgac gatcccgcgc
     8161 tcgaccgcct cgtcggcgag gccctgagcc aggggctcga cgtccggctg gcccaggcgc
     8221 gggtgcagca ggcgcgggcg agtcgccaac tggcggaggc ggggctgtct cccaccgttg
     8281 ccgcgtcggc gggcggcacg cgcagcaaga gctcccaggc ggtggcggcg gtgccgacgc
     8341 gcacgctcta cgatgcgggc ttcgacgcga gctgggagct cgatctcttc ggcggcacgc
     8401 gtcgcggcat cgaggcggcc caggcggacg tggcggcgag ccaggcgagc ctggaacagg
     8461 tgcgggtgac gctggtcgcc gaagtggcgc gcaattacgt ggacctgcgc gccgcccagc
     8521 agcgcatccg catcgcccgc gacaatctgg cgagccagcg cgagaccctg cagatcaccg
     8581 agtggcgcta tcaggccggg ctggcgcggg ccagcgaggt ggagcaggcg cgcaccgccg
     8641 aggcccagac gcgggcgggc attccggatc tggagatcga cctggtcgcc gcgcagaacc
     8701 ggctcgccgt gctcctcggg cgtgcgccgg gcagcctgca cgatcaactg gcggccgtga
     8761 agccgctgcc cgccttgccc acgcagctcg ccaccggcat tccggcggac gtgctgcgcc
     8821 agcgcccgga catcgtggcc gccgagcgca ccctggcggc ggaaacggcg cgggtcgggc
     8881 agaagctggc ccagcgcttc cccagcctga gcctgtccgc gtccttcggc tggcaggcct
     8941 acagcaccgc cgccctgggc ggggccgcca acgcggcccg cgcgctgggc gccaccctgg
     9001 ccaccaccct gttcgacggc ggccgcctgc gcagccaggt ggacgtgcag agcgcggtgc
     9061 aggaacaggc gctggtggcc taccagagca ccgtattgac ggccctggag gaggtggaga
     9121 acgccctcaa gagccacgcc gcggcgcggg aacgcttcgc cgcgcggcag gcggcggccg
     9181 aggcggcacg taatgcggcc gacctgtcgc gcaagctcta tcaagcgggg ctggcggact
     9241 tccagcaggt gctggagacc cagcgcaccc agttgtcggc ggaagacagc ctggccaccg
     9301 ccgacgccag cctgcgcacc agcctcatca gcctgtacaa ggccctcggc ggcggatggg
     9361 atgccgctgc ccggaacgga acatcatgaa cgacacctct tcctccgaca gtctcaagca
     9421 gttgctcgac caggcgcccc gcggtgggcg cggcgtggcg tggcgacgct ggctttgggc
     9481 cggcggcctc gtcctcgtgc tgggcggcgt ctttgtggcc ctgcgtggcg acaagggcgc
     9541 gggcgggcat tacctcaccg aggccgccga gcggggcgca ctggtggtga cggtgtccgc
     9601 caccggcacc ctgcagccca ccacctcggt ggacgtgggc agcgagcagt ccggcaccct
     9661 cgccgaggtg ctcgttcagg aaaacgaccg ggtgaagcag ggccagctgc tggcgcgcct
     9721 cgatctggcc aagctccagg acgcggtgag caagtcgcgg gcggcgctgg cggcggcgga
     9781 agcctcggtg gcccaggcgc gggcgacggt ggcggaatcc cgcgcctcgc tggcgcgcat
     9841 gcgccaggtg gccgagctct ccggcggcaa ggtgccgtcc aagaccgaac tggagaccgc
     9901 cgaagccacc ctggagcggg ccgtggccaa cgaggccagc gcgcgggcca cggtgatgca
     9961 ggcccaggct gccctcaaga ccgacgagac caatctgtcc aagggcacca tccgttcgcc
    10021 ggtgaatggc gtggtcctgt cccgcaaggt ggagcccggc cagagcgtgg tggcggccat
    10081 gaccatcccc gtgctgttca ccctcgccga agacctgacc gccatggagc tccaggtcaa
    10141 ggtggacgag gccgacgtga gcagcgtgcg cctgggccag ccggccagct ttacggtggc
    10201 cgcgtggccg gcgcggacct tcccggcgac catcgaacgg gtcggcatcg gctccaccat
    10261 caccgacaac gtggtcacct acaagaccgt gctggccgtc aagaacgacg acctggccct
    10321 gcgtccgggc atgacggcca ccgcctccat cgtcaccgcc aaacgggacg acgccctgct
    10381 ggtgcccaac gcggccctgc gcttcacgcc cccggcccgc ggtgcggacg cccgccaggg
    10441 cagcttcgtc tccagcctgc tgccgcgccc gccctccgag accaagcatt ccaccgcagc
    10501 ccccgccggc agtccccagg tgtgggtgct gggcgacgac ggcccgcggg ccgttccggt
    10561 gaagaccggc gtcagcaacg ggcgccagac cgagatcctg ggcggcgagc tgaaacccgg
    10621 catggcggtc atcaccgaat accaggaagg ccgcccatga gcgccgagcg cgccgagccc
    10681 ctcatccggc tcgtcggcgt caccaagacc tacggcgagg gcggcgtggc ctaccaggct
    10741 ttgcgcggca tcgacctgga ggtggccgcc ggcgagttcg tggccatcat gggccccagc
    10801 ggatcgggca aatccacggt gatgaacatc ctcggctgcc tggatacgcc caccagcggc
    10861 gcctatctct tccgcggcat ccacgtggag caactggacc gcaaccagcg cgccctgctg
    10921 cgccgccaca gcctgggctt cgtgttccag ggcttcaatc tgctggcgcg cacctccgcc
    10981 caggagaacg tggagctgcc cctgctgtac cggggcgaaa cggcgccggc gcgccacgcc
    11041 gcggcccgcg cggccctcga ccgcgtcggc ctggcgggct gggaacccca caccccggcc
    11101 gaactctccg gcggacaaca gcagcgcgtc gccatcgcgc gggccatcgt cacccagccg
    11161 gacgtgctgc tggccgacga acccaccggc aacctggaca gccagcgcag ccacgagatc
    11221 atggcgctcc tcgtcgaact caaccgcaag cagggcatca ccgtgctgat ggtgacccac
    11281 gagcccgaca tggcggccta cgcccggcgc atcgtcacct ttgtcgatgg ccgcgtggcg
    11341 cgggacgaag ccgttccgga gcgggcccta tgagcagcgt gctggtcagc gccttcctcc
    11401 tcgccctgcg ggagatccgc cgcaacctga tgcgctcctt cctcaccgtg ctgggcatcg
    11461 tcatcggcgt ggcggcggtg gtgaccatgg tcaccctcgg caacggcgcc acgcgggccg
    11521 tgtcggacca gatctccagc ctcggcagca acctcttgat ggtccgtccc ggccagcgcc
    11581 tcggccccgg ccgcgacagc gcgggcgcgc cggccttcaa acgggccgac gccgaagccc
    11641 tgcgcaccca gctcaccggc atggacgaag tggtgccggt ggcgggcagc tccgtcaccg
    11701 tggtggccgg caacaacaac tggtccacca ccgtgagcgg caccaccaac ggctacctca
    11761 aggccggcaa ctggaagatc gccagcggcc gcgcctttca ggacgacgag gagcgcgccg
    11821 gcaagtccgt gtgcgtcatc ggcgccaccg tgcggcgcga actctttggc acccgcaacc
    11881 ccctgggcga cgagatccgc gtcaagacct tcgcctgcca ggtggtgggc ctgctggccg
    11941 ccaaagggca gggcgccatg ggcatggacc aggacgacac cgtgctgatg cccctcgcca
    12001 ccgtgcagcg ccgcctcacc ggcacccagg acgtgtccac attgatggtg tccctgcacg
    12061 acggcgtgga cgccaaaccc gtcatggccc agatccgcgc cctgctgcgg gaacgccgca
    12121 agctggccga caacgaggcc gacaacttca acgtgatgga caccaaacag atcgccgaga
    12181 ccctctccgg caccatccgc accatgaccg gcctgctcgg cgccgtggcg gccgtgagcc
    12241 tgctggtggg cggcatcggc atcatgaaca tcatgctcgt gtccgtcacc gaacggacgc
    12301 gggagatcgg catccgcctc gccatcggcg ccctggaaca cgaggtgctg ctgcagttcc
    12361 tcatcgaagc cgtggtgctc tccgcctgcg gcgggctcat cggcatcctc ctcgccctgc
    12421 tcgcctgcgt cgccctctcc gccctgctga gcatgccctt cctcttcaac accggcatca
    12481 acctgctggc cttcctcttc tccgccctca tcggcgtcgt gttcggctac atgcccgccc
//
LOCUS       DAOQMM010000107        12319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2266_length_12319_cov_52.997798, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000107 DAOQMM010000000
VERSION     DAOQMM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12319
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2266_length_12319_cov_52.997798"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLH85_14415"
     CDS             complement(<1..767)
                     /locus_tag="PLH85_14415"
                     /inference="COORDINATES: protein motif:HMM:NF033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retention module-containing protein"
                     /protein_id="HNB65808.1"
                     /translation="MASTPSIATVLHVTGKAFARDTAGHLRAIRPGDSVREGEVVVTR
                     DGATVELTLANGEHLAVKPDQSVKLDPELLGSGHVDVSENAVAADSIKDVLRLLSEGK
                     DLDLDAPGAGLPGGGVGDEGHNFVRLVRILENVSPLAFAFGDPQATDVQGLAAENSTL
                     TVPAAPTVTILQDANNDGVLNAAELGTATTVAVRVGLPAGAKVGDVIVLSDGSGEQRH
                     VVTGADLTAGHWDTVVARPAEASTLSVSATLVDPAGSV"
     gene            complement(895..1530)
                     /locus_tag="PLH85_14420"
     CDS             complement(895..1530)
                     /locus_tag="PLH85_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB65809.1"
                     /translation="MKTNQWAVSRRPDLLDRWRSAFPGGGTLSPEAVTGRAFDRPAVL
                     WVDVCQDGGVSEALIASLHSLHPDIPIVALDAEPDAAGALRVLQAGAAGYCHRLAAPE
                     IFHQVATVVRHGGLWLRPELMGRVLGATFAVTSRPASVPAGLQRLSPREREAVLAVAR
                     GGTNKEVARQLGITERTVKAHLSAAFEKLGVRDRLQLVLALGRDAQLLPAA"
     gene            1717..2385
                     /locus_tag="PLH85_14425"
     CDS             1717..2385
                     /locus_tag="PLH85_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB65810.1"
                     /translation="MSALLPSSPDTARPALIAPVLLLTRDAALAARWQSVSAHPQVAT
                     DTDALTGWHAAGRRLVLADLRLRDAHRVPLWESPAWRAHGSGLAVMALSMAPSDDEGL
                     AVLQAGACGYGHALLPATHLHQALEIIEKGQLWVGRALLARLLHRVDERLRPTSPPAW
                     AVPLTPREQEVARLAAQGASNSVIATTLGITERTVKAHLTAVFAKLEVADRLQLALRV
                     HGVR"
     gene            complement(2401..4320)
                     /locus_tag="PLH85_14430"
     CDS             complement(2401..4320)
                     /locus_tag="PLH85_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HNB65811.1"
                     /translation="MSLLRQLWVSVVVAMAVVLSGAFGISLITARNYFEQQLLAQAND
                     GAVSLALSMSQQSKDRATLELLVAALFDGGHFRSIRLTDPAGQVIVERNQPDTAEGVP
                     AWLPDLLPMEARPGQALVSDGWHQLGQVTVTAARGYAYRALWKGCLQLGSLMLAIGLF
                     WAVAVSALLRWVRRPLQDMAEQAEAVGAGHFRILAEPRVIELRSMARALNHMSERVQS
                     LFAEQAGRIESLRDEATHDPVTGLFNRASFFGELRRALSDVTAPPSGTIGLLRVDDLG
                     RVNKTLGRARTDEWLAALAEALRTSQDGLEEILLARIGGADFAFLHPGGDSDAAQQTL
                     ERARAAVKALDLRLESDEEPMRLVGAATVYRQAEAITDLMARLDLCLMQAESGDSPAH
                     ATVGDLPVPGQEGWRGALEHALAERRFALAEYPLLANDGRLIHREFMLRLTTAEGSVM
                     SAGHFMPAAVRVGLAAACDLEALRLALLRLPEGADPVAVNVAPRSLTEPGFLDRLDVL
                     LAGAGSAASRVAVEVSERGLDARLAGLEALADVLARHGAALGIEHFGRQLAALPRLYA
                     LRLSYLKLDGSFVAGLPDNEANQRLVKAIVDVAHGLHVAVYAEQVYSAEEWAAAAALG
                     VVGMTGPEASRRAGR"
     gene            complement(4373..4951)
                     /locus_tag="PLH85_14435"
     CDS             complement(4373..4951)
                     /locus_tag="PLH85_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HNB65812.1"
                     /translation="MRLLAQQRYGAAAAEAVEVWRGSMASWRDLPEEDQLRRVNEFFN
                     RRVRFVDDVEAWGQADYWATPLEVLARGVGDCEDFVIAKYFSLRDLGVSDARLRLTYV
                     KARVGGPGSSVFLAHMVLSYYPTPDSVPLILDNLIRDIRPASSRPDLAPVYSFNSQGI
                     FVGTANQVASPVDRVSRWPELLVRMRAEGYEP"
     gene            complement(5022..5312)
                     /locus_tag="PLH85_14440"
     CDS             complement(5022..5312)
                     /locus_tag="PLH85_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65813.1"
                     /translation="MNGFYAPEYAQNAEYLFIPAPVTLLLRGTYELFTFVGRFARFLN
                     FRGLAPMFGEIQGPVLHARLISVSCHDPCALCARIPDECILRRLPSLDKRHP"
     gene            5380..7197
                     /locus_tag="PLH85_14445"
     CDS             5380..7197
                     /locus_tag="PLH85_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNB65814.1"
                     /translation="MFIAKTLPACLSIAFATCASTAACAAGTLPDAVEKAILRNPEVL
                     ARLHEYQAADAERDVARGGYFPRVDLQAYTGRERRDTPVTPTTTYSRPGAQLQLRQLL
                     FDGFATRNDVKRLGYARAVRYFELLGTSDDTALEASRAYLDVLRYRELSDLAKENWAV
                     HKETVDQIERRVKAGVGRRVDLEQAFGRLALAQSNWLTETSNLHDVSRRFERVVGDAP
                     DERLAPAPVFSDKLPGDKEVIAQAVRNNPGFRAAVANIRASRANVDVQRANYAPTVQL
                     LASTGMDKNLLGVTGETNTSAVQVLLNWNLLKGGSDEARIRQSHEQLYTATDLRDKAC
                     RDVRQTTAIAWNDVRKLKEQLTYLEQHQLSTEKARDAYRQQFDIGQRSLLDVLNTENE
                     LFEAKRATANARFDMTLAELRVLASAHQILPAVQLAPIAKTGPDEAGATEPADDEALR
                     CSADVPVVAPLDTAGALQGRPQPAATPLPATTAMPVVAPNAATAPLSPLEPLLKSWAA
                     AWSAKDLKAYRSFYSSQFKPDQGTPEAWLAMREKRVTKPGSIAVEVDKLSTRNLSATS
                     AEASFVQHYTSSGYSDVMNKTLQFQLENGQWKIVRESNR"
     gene            complement(7267..8370)
                     /locus_tag="PLH85_14450"
     CDS             complement(7267..8370)
                     /locus_tag="PLH85_14450"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNB65815.1"
                     /translation="MSDITSHAHLAPAVSQLPVSWYFDEKIFELEKRLLFDAGPGYVG
                     HALMVPEVNQFRSLEWLDHAQMLVQRDDGFYRMSNICRHRQAIMMEGSGTAERNIVCP
                     IHRWTYNPDGLLIGAPHFQNNPCLNLDRHRLENWHGLLFKGPRSANVDLGGMNVAQDL
                     DFSGYKLDRVEIHECNYNWKTFIEVYLEDYHVEPYHPGLGQFVTCDDLTWQFGEWYSV
                     QRVGITSLKKPGSMTYERWHQAVLTYYNGELPRHGAIWLTYFPNIMVEWYPHVLVVST
                     LIPTDVHKTTNVVEFYYPEEILEFERDFVEAEQAAYMETAIEDDDIGERMDRGRLALL
                     KEGRNEVGPYQSPYEDGMQHFHEFYRRIIQPHL"
     gene            8494..8727
                     /gene="xseB"
                     /locus_tag="PLH85_14455"
     CDS             8494..8727
                     /gene="xseB"
                     /locus_tag="PLH85_14455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNB65816.1"
                     /translation="MVKTKASTPPSFETAVAELESIVQEMESGQLPLDEALTRYQRGT
                     ALLRHCQDVLDKAEQTLRTIEAGAPAAAGDSDA"
     gene            8724..9617
                     /locus_tag="PLH85_14460"
     CDS             8724..9617
                     /locus_tag="PLH85_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB65817.1"
                     /translation="MNTSFAHWMADVQARTEAALDRALPAAGIAPERLHDAMRYAVLG
                     GGKRVRPLLVHAAGLLSGATPEHLDRAACAVEMIHAYSLVHDDMPCMDNDVLRRGKPT
                     VHVQYDEATALLVGDALQTQAFLHLAEAPVCDDPMRQIGMLRLLAQASGSRGMAGGQA
                     IDLDSVGSQLSLAELEFMHIHKTGALIRAALLLGVQCGRALPETELDNLDHVGKVIGL
                     LFQVVDDILDTEADTATLGKTAGKDAAHDKPTYVSLLGVVRARELAGELCQDALDRLA
                     RFGDSAHRLCELTRFIVQRQF"
     gene            9652..11550
                     /gene="dxs"
                     /locus_tag="PLH85_14465"
     CDS             9652..11550
                     /gene="dxs"
                     /locus_tag="PLH85_14465"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNB65818.1"
                     /translation="MPSATSPATPRTPLLERIESPADLRKLAPAELKPLADELRSFLI
                     DSVSRTGGHLSSNLGTVELTIALHYVFNTPEDRIVWDVGHQTYAHKILTGRREAMAGL
                     RQYHGISGFPRRCESPYDTFGTAHSSTSISAALGMAEAARLRGEDRRAIAVIGDGAMS
                     AGMAFEALNNAGDIHDTNLLVILNDNEMSISPPVGALNKHLSRLLSGRIYNGARKIGK
                     RFLENLPPPVFELAKRTEEHVKGMMMPGTLFEEFGFNYIGPIDGHDLDALLPTLQNLR
                     TLKGPQFLHVITRKGQGYKLAEADPILYHGVSKFDHAAGIATGKDAKGGGKLTYTQVF
                     GDWLCDMAAQDERLVAITPAMREGSGMVRFAREFPSRFYDVGIAEQHALTFGAGLACE
                     GFRPVVAIYSTFLQRAYDQFIHDVALQNLPVMMAIDRAGLVGADGATHHGAFDLSYLA
                     CIPNLTLFCPADEDECRQMLYTAYHHNGPTAVRYPRGSGLGIAPQAAMHTLPIGKGEI
                     RREGRRTALLAFGSLLAPALQVGDALDATVANMRFVKPIDRNLILDLAARHELLVTLE
                     ENAVIGGAGSEVARVLAEAGVPVRLIQMGLPDRFVDHGDQTQLMAEVGLDAAAICARI
                     AQVYPQNS"
     gene            11631..>12319
                     /gene="folE2"
                     /locus_tag="PLH85_14470"
     CDS             11631..>12319
                     /gene="folE2"
                     /locus_tag="PLH85_14470"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNB65819.1"
                     /translation="MTVQQTPSAIPDVQNSADSRQLAINKVGIKSIRHPIKVLDKNGG
                     VQHTIAVFNMYVGLPHNFKGTHMSRFVEILNSHEREISVESFEPMLREMVKKLEAETG
                     HVEMTFPYFINKAAPVSGVQSLMDYEITFIGEIRAGGLYEFTMKVVVPVTSLCPCSKK
                     ISAYGAHNQRSHVTVTATTNDHLWIEELVQLVEGQASCELYGLLKRPDEKFVTERAYD
                     NPKFVEDMVRDV"
BASE COUNT         2271 a         4428 c         3658 g         1962 t
ORIGIN      
        1 acactgcccg caggatccac gagggtggct gacaccgaca gggtcgaagc ttcagcgggg
       61 cgcgcgacca cggtgtccca atgccccgcc gtcaggtcgg cgccggtgac gacgtggcgc
      121 tgttcgccgc tgccgtcgct caggacgatc acgtcgccca ccttggcccc ggccggcagc
      181 ccgacacgga ccgcgacggt ggtggccgtg cccagttctg ctgcgttcag cacgccatcg
      241 ttgttggcgt cctggagaat cgtcaccgtc ggcgcagcgg gcaccgtgag cgtgctgttc
      301 tcggcagcca gcccctgtac gtcggtcgcc tggggatcgc cgaaggcaaa ggccagcggc
      361 gagacgtttt ccaggatgcg caccagccgc acgaagttat gcccttcgtc gccgacgccg
      421 ccacccggca ggccggctcc cggggcatcg aggtccagat ccttgccttc gctcagcagc
      481 cgcagcacat ccttgatgct gtcggccgcc acggcatttt cggagacatc cacgtgaccg
      541 gagccgagca actcgggatc cagtttcacc gactggtcgg gcttgacagc caggtgctcg
      601 ccgttggcaa gggtcagctc caccgtggcg ccgtcacgcg taacgaccac ctcgccttcc
      661 cgcacgctgt cccccggacg gatcgccctg agatgaccgg cggtgtcgcg cgcaaaggcc
      721 ttgccggtga cgtgaaggac ggtggcgatg gaaggggtcg aagccatgac agtctccagg
      781 agggttgccg gcgatcggac cggcagacgg aatgcgtatc gggatgcagt ctagcggtcg
      841 gcgacaagca cttgaattgt cctggtggac aatgcacgcg ggccgacgcg gaactcaggc
      901 cgcagggagc agctgtgcgt cgcgccccag tgcgagcacc agttgcaagc gatcgcgcac
      961 ccccagtttt tcaaaggctg cgctgaggtg ggccttgacg gtccgttcgg taatgccgag
     1021 ctggcgggcg acttccttgt tggtcccacc ccgtgccacg gcgagcaccg cctcccgctc
     1081 tcgtggcgag agacgctgaa gcccggccgg cacgctcgca ggacgggacg tcaccgcaaa
     1141 ggtggcgccg aggacccgcc ccatcaattc cggccgcagc cagagtccgc catggcgcac
     1201 gaccgttgcc acctgatgga agatctcggg cgcggccagg cggtggcaat accccgctgc
     1261 gccggcctga aggacccgga gcgcgcccgc cgcgtccggc tcggcatcga gcgccacaat
     1321 cggaatatcc ggatggaggc tgtgcaggga cgcgatgagc gcctcggaga caccaccgtc
     1381 ctggcagaca tcgacccaca acacggccgg gcggtcaaac gcacgccccg tcaccgcctc
     1441 gggtgacaag gttccgcccc ccggaaaggc gctccgccac cggtcaagga gatccggcct
     1501 gcggctcacc gcccattgat tcgtcttcac gtgatccggc acgtcccccc cgcacggcta
     1561 gaatcggact cttcccggcg ctgcgtcgcc tccgcggcat cgccgcggcc acgcgacgtc
     1621 acataaattg acgcgccgga tcgtcccttg atgtagacct tacggatttg caggcaaatt
     1681 acttgagtcc ggccctgcac atcccatttc acccgcatga gcgccctcct cccatcctcc
     1741 cccgacactg cccggccagc cctgattgcc cccgtcctgc tgctgacccg cgatgcagcc
     1801 ctcgcagcac gctggcaatc cgtcagcgct cacccgcagg tggcaacgga caccgacgcc
     1861 ctgacgggtt ggcatgccgc gggtcggcgc ctggtgctgg ccgatctgcg tcttcgggac
     1921 gcgcaccgcg tccccctgtg ggaaagcccg gcatggcggg cgcacgggtc cgggttggcc
     1981 gtgatggccc tgtcgatggc gccatccgac gacgaaggcc tcgccgttct gcaggccggc
     2041 gcctgcggtt atggacacgc gctgctgcct gccacacacc tgcatcaggc gctggagatc
     2101 atcgaaaagg gtcagctctg ggtcgggcgc gccctgcttg cgcgtctgct gcatcgggtc
     2161 gacgaacgtc tgcgcccgac gtcgccgccg gcatgggccg taccgctcac accccgtgaa
     2221 caggaggtcg cgcgtctcgc cgcacagggc gccagcaaca gcgtcattgc cacgacactg
     2281 ggaatcaccg agcggaccgt caaggcccac ctgacagcgg tgttcgccaa actggaggtt
     2341 gccgaccgcc tgcagctggc tttacgtgtc cacggagtac gttgatgcgc cctgctcgcg
     2401 tcagcgaccc gcccggcgcg acgcctccgg cccggtcatg ccaaccaccc ccagcgccgc
     2461 cgctgcagcc cattcctctg cgctgtacac ctgctcggca tagacggcca catgaagtcc
     2521 atgggcgaca tccacaatgg ctttcacgag gcgctggttc gcctcgttat cgggcagacc
     2581 ggccacgaaa ctgccatcca gcttgagata ggacaggcgc aaggcataca ggcggggcaa
     2641 ggcggccagc tggcgcccga agtgttcgat ccccagcgcg gccccgtgac gggccagcac
     2701 gtcggccaag gcctccagcc cggccagacg ggcgtcgagc ccccgctcgc tcacctccac
     2761 cgccacgcgc gatgccgcgc tgccggcccc cgcgagcaac acgtccaggc ggtcgaggaa
     2821 gcccggctcc gtcaatgacc ggggtgcgac attcacggcc accgggtcgg ccccctccgg
     2881 gaggcgcagc agcgccaggc gcaaggcctc cagatcgcac gccgccgcca ggcccacccg
     2941 caccgcagcc ggcatgaagt ggccggcact catcacgctc ccctccgcgg tcgtcagacg
     3001 cagcatgaac tcccggtgga tcagacgtcc gtcgttggcc agtaaggggt actccgccag
     3061 ggcgaagcgg cgttcggcca aggcgtgctc cagcgcgccg cgccagcctt cctgccccgg
     3121 aaccgggaga tcacccactg tcgcgtgggc cgggctatcg ccactttccg cctgcatcag
     3181 gcacagatcg aggcgcgcca tcaggtcggt gatcgcctca gcctgacggt agaccgtcgc
     3241 cgcgcccacg agacgcatcg gttcctcgtc actctccagg cgcaggtcca gcgccttcac
     3301 cgcggcgcga gcccgctcga gggtctgctg cgccgcatcg gaatcccctc cgggatggag
     3361 gaatgcgaaa tcggcgcctc cgatgcgtgc cagcaggatc tcctccagcc cgtcctggga
     3421 ggtgcgcaag gcctcggcca gcgccgcaag ccattcatcg gtccgggcgc gcccgagcgt
     3481 cttgttgacc cggccgagat cgtccacccg cagcagcccg atggtgccgg aaggcggcgc
     3541 ggtgacatcg ctcagcgccc gccgcaattc accgaagaag ctggcgcgat tgaacaggcc
     3601 ggtcaccgga tcatgggtcg cctcgtcacg cagtgactcg atgcgccctg cctgttcggc
     3661 aaacagagac tggacccgct cggacatgtg attgagggcg cgtgccatgc tgcgcagttc
     3721 gatcaccctc ggttcggcca ggatacggaa atgtccggcg ccgaccgcct ccgcctgctc
     3781 tgccatgtcc tgcaacggcc gtcgcaccca ccggagcagc gccgacaccg ccaccgccca
     3841 gaacaggccg atggccagca tcaggctgcc cagctggagg cagcccttcc aaagggcgcg
     3901 gtaggcgtag ccccgtgcgg cagtgaccgt cacctgaccg agctggtgcc agccgtcgct
     3961 caccagggcc tgtccggggc gggcctccat cggcagcaga tccggcagcc acgccggcac
     4021 gccttcggcg gtgtccggct ggttacgctc cacgatcacc tgaccggcgg gatcggtgag
     4081 gcggatcgag cggaaatggc cgccatcgaa caaggccgcc accagcagct ccagcgtcgc
     4141 ccggtccttg ctctgctggg acatggacag ggccagggac accgccccgt cgttggcctg
     4201 ggcaagcagc tgctgttcga agtaattgcg cgcggtgatc aggctgatgc cgaaggcgcc
     4261 gctcagcacc accgccatcg ccaccaccac gctgacccac aactgtctca agagcgacat
     4321 gaccggcctc cgatccacca tccaggcccg ccctgcgcgg ggctcatcac gttcatggtt
     4381 catatccttc cgcgcgcatg cgcacgagca gctccggcca gcgtgacacc cgatccaccg
     4441 gggacgcgac ctgattggcc gttccgacga agatgccctg ggaattgaaa ctgtataccg
     4501 gggccagatc cggtcgcgaa cttgccggcc ggatgtcccg gatcagattg tcgagaatca
     4561 gcggtaccga atctggcgtc gggtaatagc tcaggaccat gtgggccagg aacaccgagc
     4621 tgccgggtcc gccaacccgc gccttgacgt aggtcagtcg caggcgcgcg tccgaaacgc
     4681 ccaggtcacg aagggaaaaa tacttggcga tcacgaaatc ctcgcaatcc cccacgcccc
     4741 gcgccagcac ttccagcggc gtcgcccagt agtcggcctg accccaggcc tccacatcat
     4801 ccacgaagcg cacgcgccgg ttgaagaact catttacccg gcgcagctga tcctcctcgg
     4861 gcaggtcccg ccagctcgcc atgctcccac gccagacctc cacggcctcc gcggccgcgg
     4921 caccgtaccg ctgctgagcc agcaggcgca tgcggtcacc gttcagcccg gaccaccccg
     4981 cacgtcccag caccaggacc attgtggccc ccagcacacc gtcacggatg ccttttatcc
     5041 agcgagggta atcggcgcaa aatacattca tccgggatac gcgcgcacaa tgcgcacggg
     5101 tcatgacagg aaactgaaat cagccgcgcg tggagcaccg gcccttgaat ctctccaaac
     5161 atcggcgcaa gtccgcgaaa attaaggaat cgcgcaaagc ggccgacaaa tgtaaaaagt
     5221 tcatacgtac ccctcaaaag cagggtgaca ggggcaggaa tgaacaaata ttccgcattc
     5281 tgcgcatatt ccggcgcata gaatccattc atgcggacgt tgtacgttga tgcgcctgga
     5341 cgcgcaaacg ttcgattcac cggaaaaaat cacaacatca tgttcattgc caagactttg
     5401 cccgcctgtc tgagcatcgc attcgcgacc tgcgccagta ctgctgcatg cgccgccggc
     5461 acgctgccgg acgccgtcga aaaggccatc ctgcgcaacc ccgaggtgct tgcgcggctg
     5521 catgaatatc aggccgccga tgcggaaagg gatgtggcgc gcggcggcta ttttccacgg
     5581 gtcgatcttc aggcatacac cgggcgcgag cgccgcgaca cgcccgtcac gccgaccacc
     5641 acctacagcc gtccgggcgc ccagctccag ctgcgtcagc tgctcttcga cggtttcgcc
     5701 acccgcaacg acgtcaaacg cctcggctac gcccgcgccg tgcgctactt cgaactcctc
     5761 ggcaccagcg acgacacggc gctggaagcc tcgcgcgcct atctcgacgt cctgcgttac
     5821 cgcgagctgt ccgatctggc caaggaaaac tgggccgtcc acaaggaaac cgtcgatcag
     5881 atcgagcgcc gcgtgaaggc cggcgtcgga cgccgcgttg acctcgaaca ggcctttggc
     5941 cgcctcgccc tggcgcaatc caactggctc accgaaacct ccaacctgca tgatgtcagc
     6001 cgccgcttcg agcgcgtggt cggcgacgca ccggatgaac gcctggcgcc ggcgccggtg
     6061 ttctccgaca agctgccagg cgataaggaa gtgatcgccc aggccgtccg caacaacccc
     6121 ggcttccgcg ccgccgtcgc caacattcgc gcctcccgcg ccaatgtgga cgtccagcgc
     6181 gccaactacg cgcccacggt gcagctgctg gccagcaccg gcatggacaa aaatctgctc
     6241 ggcgtcaccg gcgagaccaa caccagtgcg gtgcaggtgc tgctcaactg gaatctcctc
     6301 aagggcggct ccgacgaagc gcgcatccgc cagtcccacg aacagctcta caccgccacc
     6361 gacctgcgcg acaaggcctg ccgcgacgtc cgccagacga ccgccatcgc gtggaacgac
     6421 gtgcgcaagc tcaaggagca gctcacctac ctcgagcagc accagctctc caccgaaaag
     6481 gcccgcgatg cctatcgcca gcagttcgac atcgggcagc gcagcctgct ggacgtgctc
     6541 aacactgaaa acgagctgtt cgaggccaaa cgcgccacgg ccaatgcccg cttcgacatg
     6601 acgctggccg aattgcgcgt gctggccagc gcccaccaga tcctgccggc ggtgcagctc
     6661 gcccccatcg ccaagaccgg tcccgacgag gcgggcgcca cggaacccgc tgacgacgaa
     6721 gctctgcgct gcagtgccga tgtgccggtg gtcgccccgc tcgataccgc tggcgccctg
     6781 cagggacgcc cgcaacccgc cgcgacaccg ctgcccgcaa caacggccat gccggttgtt
     6841 gcgccgaacg ctgcgacggc tccgctctcg ccgctcgaac cattgctcaa atcctgggcc
     6901 gcagcctggt ctgccaagga tctgaaggct tatcgcagct tctactcgag ccagttcaaa
     6961 ccagaccagg gtacgcccga ggcctggctg gccatgcggg aaaagcgcgt caccaagccg
     7021 ggcagcatcg ccgtcgaggt ggacaagctc agcacccgca acctgtccgc cacctcggcc
     7081 gaagccagct tcgtccagca ctacacatcg tccggctact cggacgtgat gaacaagacg
     7141 ctccagttcc agctggagaa cggtcagtgg aagatcgtgc gggaatccaa tcgctgagtc
     7201 tgcgtcaccg caaacgtgca aagggagcca cacggctccc tttgcgtttc aagctgccgg
     7261 cggtcttcag agatggggct ggatgatgcg gcgatagaac tcgtggaagt gctgcatccc
     7321 gtcctcatag ggcgactgat acggccccac ctcgttacgg ccttccttga gcagcgccaa
     7381 tcgcccccgg tccatgcgct caccgatgtc gtcgtcctca atggccgttt ccatgtaagc
     7441 ggcctgctcg gcttcgacga agtcccgctc gaattccagg atctcctcgg ggtaatagaa
     7501 ctcgaccacg ttggtggtct tgtgcacgtc cgtcgggatc agcgtactca ccacgagcac
     7561 atggggatac cactccacca tgatgttggg gaagtaggtc agccagatcg cgccatgccg
     7621 gggcagttca ccgttgtaat aggtgagcac tgcctgatgc cagcgctcgt aggtcatgga
     7681 ccccggcttc ttgagggagg tgatgcccac ccgctgcacc gagtaccact ccccgaactg
     7741 ccaggtcaga tcgtcgcagg tgacgaactg gccaagtccg ggatggtagg gctccacgtg
     7801 ataatcttcc agatagacct cgatgaaggt cttccagttg taattgcact catggatctc
     7861 gacgcggtcg agcttatagc ccgagaaatc cagatcctgc gccacattca tcccgcccag
     7921 atccacgttg gccgaacggg gccccttgaa cagcaggccg tgccagtttt ccagcctgtg
     7981 gcgatccagg ttgaggcagg ggttgttctg gaaatgcggc gcaccgatca acaacccgtc
     8041 cgggttgtaa gtccagcgat ggatcgggca gacgatgttg cgctcagccg ttccggagcc
     8101 ctccatcatg atcgcctggc gatggcgaca gatgttggac atgcgataga acccgtcatc
     8161 gcgctgcacc agcatctgcg cgtgatcgag ccactccagg gaacggaact ggttcacttc
     8221 cggcaccatc agcgcgtggc cgacgtagcc gggaccggca tcgaagagga ggcgtttctc
     8281 caattcgaag attttttcgt cgaaatacca cgataccggc agttgggaaa ctgccggcgc
     8341 cagatgtgca tgggaagtga tgtcagacat cccgaacccc cttttcgtaa acgaaagccc
     8401 gaaacgtaaa acggagtatt atatagcatc accaatttga ccagtagggc ttgttgcgct
     8461 atttttaccg tttgccctga gcggtcccgg cccatggtca agactaaagc ctccaccccg
     8521 ccgagtttcg aaacggctgt cgccgagctg gagtccatcg tccaggaaat ggaatccggc
     8581 cagttgcccc tcgatgaagc cctgacccgc taccagcgcg gtaccgccct gctccgccat
     8641 tgccaggacg tcctggacaa ggccgagcag acccttcgca ccatcgaggc cggtgcgccg
     8701 gccgccgcgg gtgactccga cgcatgaaca cctctttcgc ccactggatg gccgatgtcc
     8761 aggcccgcac cgaagccgct ctcgaccgcg ccctgccggc cgccggcatc gcaccggaac
     8821 gccttcacga tgccatgcgt tacgccgtac tcggtggcgg caagcgggtt cgcccgctcc
     8881 tcgtccacgc ggcaggcctc ctgtcgggcg ccacgccgga acatctcgac cgggccgcct
     8941 gcgccgtcga gatgatccac gcctattccc tcgtgcatga cgacatgccc tgcatggaca
     9001 acgacgtcct gcgccgtggc aaacccaccg ttcacgtcca gtacgacgag gccaccgcac
     9061 tgctcgtcgg cgacgccctc cagacccagg ccttcctgca cctggccgag gcacccgtgt
     9121 gtgatgaccc gatgcgccag atcggcatgc tgcgcctgct cgcccaggcc tcggggtccc
     9181 gcggcatggc cggcggtcag gccatcgacc ttgactcggt gggcagtcag ctcagtctgg
     9241 ccgaacttga attcatgcac atccacaaga caggcgccct gattcgggcc gcactgcttc
     9301 tcggggtaca atgcggccgc gcgttgcccg aaaccgaact ggacaacctg gatcacgttg
     9361 gcaaggtcat tggtctgctc ttccaggtgg tcgacgacat cctcgatacc gaggccgaca
     9421 ccgccaccct cggcaagacg gctggcaagg atgccgccca cgacaagccg acgtacgtga
     9481 gcctgctcgg agtggtccgc gcgcgcgaac tggccggcga actgtgccag gacgccctcg
     9541 accggcttgc ccgatttggc gacagcgccc atcgtctctg cgaactgacc cgcttcatcg
     9601 tgcagcgtca gttctgaacc gaatccacgt catttgccct caacgcccgg aatgccctcc
     9661 gccacgtcgc ctgccacgcc ccgaaccccc ttgctcgaac gcatcgagtc gcccgccgat
     9721 ctgcgcaagc tggctccggc cgagctcaag cctctggccg acgaactgcg cagcttcctg
     9781 attgactcgg tctcccgtac cggcggtcac ctctcgtcca acctgggcac ggtggaactg
     9841 acgatcgccc tgcactacgt cttcaacacg ccggaggacc gcattgtctg ggacgtgggt
     9901 caccagacct acgcccacaa gatcctcacc ggccgccgtg aggccatggc cggcctgcgc
     9961 cagtatcacg gcatctccgg cttcccgcgg cgctgcgaaa gcccctacga cacgtttggc
    10021 acggcccact cgtccacctc catctcggcc gccctgggca tggccgaggc agcccgactg
    10081 cgcggcgagg atcgacgcgc catcgctgtc atcggcgatg gcgccatgtc ggcaggcatg
    10141 gccttcgagg ccctcaacaa cgccggtgac atccacgaca ccaatctgct ggtgatcctc
    10201 aacgacaacg agatgtccat ctcgccgccc gtgggcgccc tcaacaagca cctgtcgcgc
    10261 ctgctctcgg gacgcatcta caacggcgcc cgcaagatcg gcaaacgttt cctggaaaac
    10321 ctgcccccgc cggtcttcga actggccaag cgcaccgagg aacacgtcaa gggcatgatg
    10381 atgcccggca ccctgttcga ggagttcggg ttcaactaca tcggccccat cgacggtcac
    10441 gacctggacg ccctcctccc caccctgcag aacctgcgca ccctcaaggg cccccagttc
    10501 ctgcacgtga tcacccgcaa gggccagggc tacaagctcg ccgaggccga ccccatcctc
    10561 taccacggcg tttccaagtt cgaccacgcc gccggcattg ccaccggcaa ggatgccaag
    10621 ggcggcggca agctcaccta cacccaggtc ttcggcgact ggctgtgcga catggcggct
    10681 caggacgagc gcctcgtcgc catcaccccg gccatgcgcg aaggctcggg catggtgcgc
    10741 ttcgccaggg aattcccgtc ccgcttctac gacgtgggga tcgccgagca gcacgccctc
    10801 accttcggcg caggcctcgc ctgcgaaggc ttccgcccgg tggtcgcgat ctactccacc
    10861 ttcctgcagc gcgcctacga ccagttcatc cacgacgtcg ccctgcagaa cctgcccgtg
    10921 atgatggcca tcgaccgggc cgggctggtg ggcgccgacg gcgcaaccca ccacggcgcc
    10981 ttcgacctgt cctacctcgc ctgcatcccc aacctcacgc tcttctgccc ggccgacgaa
    11041 gacgaatgcc ggcagatgct gtacaccgcc taccaccaca acggtcccac ggcggtccgc
    11101 tacccgcgcg gcagcggcct gggtatcgcg ccacaggcgg ccatgcacac cctgcccatc
    11161 ggcaagggcg aaatccgccg cgaaggccgc cgtaccgccc tgctcgcctt cggcagcctg
    11221 ctcgcgccag ccctgcaggt cggcgacgcg ctggacgcca ccgtcgccaa catgcgcttc
    11281 gtcaaaccca tcgaccggaa tctgatcctc gatctggccg cccgtcacga gctgctggtg
    11341 acactggagg aaaacgcggt gatcggtggt gccggttcgg aagtggcccg tgtgctcgcc
    11401 gaagcaggtg tcccggtccg cctgatccag atgggcctgc ccgaccgctt cgtcgatcac
    11461 ggcgaccaga cccagctgat ggccgaagtc gggctcgacg cggccgccat ctgcgcccgc
    11521 atagcgcagg tctatccgca gaatagttga gaaaaattgt cgggaaatga taggatcgac
    11581 gctccatggg gcgtcagatc cctttacccg tagaaacacc gagaacagtc atgaccgtgc
    11641 aacagacccc ttcagcgatc ccggacgtcc agaactccgc ggattcccgc cagctggcca
    11701 tcaacaaggt cggcatcaag tccatccgtc accccatcaa ggtcctggac aagaacggtg
    11761 gcgtgcagca caccatcgcc gtcttcaaca tgtacgtcgg cctgccccat aacttcaagg
    11821 gcacccacat gtcccgcttc gtggagatcc tcaacagcca cgagcgggag atctccgtcg
    11881 aatccttcga gcccatgctg cgcgagatgg tcaagaagct cgaagcggaa accggccacg
    11941 tggagatgac ctttccgtac ttcatcaaca aggccgcgcc agtctccggc gtgcagagcc
    12001 tgatggacta cgagatcacc ttcatcggcg agatccgcgc cggcgggttg tacgaattca
    12061 ccatgaaggt cgtggtgccg gtcaccagcc tgtgcccctg ctccaagaag atctccgcct
    12121 acggcgccca caaccagcgc tcccacgtca ccgtcacggc gaccaccaac gaccatctgt
    12181 ggatcgagga actggtgcag cttgtcgagg gtcaggcctc ctgcgaactc tacggcctgc
    12241 tcaagcggcc ggacgaaaag ttcgtcaccg agcgcgccta cgacaacccc aagttcgtcg
    12301 aggacatggt gcgcgacgt
//
LOCUS       DAOQMM010000108        12120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2330_length_12120_cov_50.406299, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000108 DAOQMM010000000
VERSION     DAOQMM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12120
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2330_length_12120_cov_50.406299"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLH85_14475"
     CDS             complement(<1..587)
                     /locus_tag="PLH85_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HNB65820.1"
                     /translation="MRFNFPVIVIDEDFRSENTSGLGIRALAEAIEKEGLQVVGVTSY
                     GDLASFAQQQSRASTFILSIDDEEFSSPEAAAKAIADLRAFVSEIRLRNADIPIFLHG
                     ETRTSRHIPNDVLRELHGFIHMYEDTPEFIARNVVREARAYMDALPPPFFRALTHYAS
                     DGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLR"
     gene            complement(699..1268)
                     /gene="dcd"
                     /locus_tag="PLH85_14480"
     CDS             complement(699..1268)
                     /gene="dcd"
                     /locus_tag="PLH85_14480"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNB65821.1"
                     /translation="MSIKSDKWIRRMAEGDAKMIEPFAPELVRQNAGGKIVSYGTSSY
                     GYDVRVANEFKIFTNINSTIVDPKAFDERNFVDFTGDVCIIPPNSFALARTVEYFRIP
                     RSVLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLF
                     FESDEICDTSYRDRGGKYLGQTGVTLPKI"
     gene            complement(1378..1563)
                     /locus_tag="PLH85_14485"
     CDS             complement(1378..1563)
                     /locus_tag="PLH85_14485"
                     /inference="COORDINATES: protein motif:HMM:NF038104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038104 family lipoprotein"
                     /protein_id="HNB65822.1"
                     /translation="MRRLRAWGPAVVLAASLSGCVVGTVVGTAVDVGVEVVKVPFKVG
                     KGVYDAVKDDDVKDAGR"
     gene            complement(1563..2654)
                     /gene="apbC"
                     /locus_tag="PLH85_14490"
     CDS             complement(1563..2654)
                     /gene="apbC"
                     /locus_tag="PLH85_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNB65823.1"
                     /translation="MSLTEQSILDSLRQYADPNTGKDYVTSRAVKNLRLHGADVSFDI
                     ELGYPAASQIDTIRQAVIACAKSVPGVGNVSANVFTRIVAHAVQQGVKLLPGVKNIIA
                     VASGKGGVGKSTTSANLALALAAEGATVGILDADIYGPSQPQMLGIGDSQPESRDGKT
                     MEPLEAHGLQVMSIGFLVDVETPMVWRGPMATQALNQLLKETNWRDLDYLVIDMPPGT
                     GDIQLTLSQSVPVTGAVIVTTPQDIALLDARKGLKMFEKVGVPILGIVENMSIHICSQ
                     CGHEEHIFGSGGGATMGEDYGVPFLGALPLDIQIRREADGGTPTVAADPDGRVAQVYK
                     EIARKVAIAVAERQKDMSHKFPSIVVQNT"
     gene            2831..4987
                     /gene="metG"
                     /locus_tag="PLH85_14495"
     CDS             2831..4987
                     /gene="metG"
                     /locus_tag="PLH85_14495"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNB65824.1"
                     /translation="MTTRKILVTNALPYANGDIHLGHLVGYIQADIWVRYQRMRGHTV
                     HYVCADDTHGTPVMLRAEKEGLTPEQLIARVHGEHLRDFTDFGVAFDHYHSTHSSENR
                     WYAEDIYTRLRDSARLIETRAIEQFYDPVKEMFLPDRFIKGECPKCGAQDQYGDNCEV
                     CGAAYAPTDLKHPRSAVSGAEPVLKTSEHYFFRLSDPRAVEFLREWTQGTTPEGERRL
                     QAEAANKMKEWLGDSGENALSDWDISRDAPYFGFEIPGAPGKYFYVWLDAPIGYFASF
                     RHLAILRGDIDVADYTEAGPAAATGTELIHFIGKDILYFHALFWPAMLHFAGYRTPTQ
                     LCVNGFLTVDGAKMSKSRGTFITARSYTEQKLNPEWLRYYFAGKSNGTMEDVDLNLDD
                     MIAKVNSDLVGKFVNIASRCAGFIAKRFDGKLAAHDSALDLAWSADEVAAAYEARDYS
                     RALRRIMEFADQANAYVNDHKPWELAKQDGQAARLHVVCSTALNLFKALSGLLKPVLP
                     ALASQVETFLRIEPLQWADVLTPLPAGHAILSYSHLMTRIERKQIDALLDANRESLAP
                     AAATPAKADKTTQPAHSEQRHAQHQAHAAKVEAAHASAFEPFIGIDDFAKVDLRIARI
                     VSAEHVDGAAKLLRLQLDIGEAQPRQVFAGIKSAYDPATLVGRLTVMVANLAPRKMKF
                     GMSEGMVLAASDESGTHAGLFILSPDAGAVPGMRVK"
     gene            5035..5316
                     /locus_tag="PLH85_14500"
     CDS             5035..5316
                     /locus_tag="PLH85_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65825.1"
                     /translation="MTERSVPTLRLPPAFFGWALVDLIGVAVMAVGAAWLIEGRASLL
                     PGFPANTLQAWICVVGGLGTLFVAAVKMLVEVMHANKRGGAANDDAFGR"
     gene            5448..7136
                     /locus_tag="PLH85_14505"
     CDS             5448..7136
                     /locus_tag="PLH85_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNB65826.1"
                     /translation="MKKISFHPKLLDTLPGYDAGQFGRDLSAGLTVGVLALPLAMAFA
                     IASGMSPTAGIWTAIVAGFLISALGGSRVQIGGPTGAFIPILYGIVVEYGVQNLLVAT
                     MLAGAMLVLMGVFRVGNVIRFIPKTVVIGFTNGIAVVIFLAQIKDFLGLRIEHLPGEF
                     FGKVHALFTHLDTLDVPTLALSVASLAVLLSWNGVARRYPALGRVPGPLGVLVVATVA
                     NAILHLPVETIGSRFGGIPQEIPPLALPDLTLATLGKLISPALTIALLGAIESLLSAR
                     VADATIEDRHDPNQELIGQGIANLIVPLFGGFAATGAIARTATNVRTGGRTPVAGMLH
                     AGVLLAIVLALAPFARHIPLATLSAIVVVVAVNMGEWHEFRGLGRFSVNYRTTLLSTF
                     LVTVVFDLTLAVELGMVLASLFFIYRMSDLTRVEREPLPELTLLPHFLYPDGSPRVVA
                     YALYGSLFFGAIHKLETLLDPAERHPEVVILDMLHLVNLDTTGLEGLESLCKNLHRKG
                     CALLLCTLNPQPESLIRRSGFAEEVGVNNLCADMYAALARAKVLLPSFMDEDNL"
     gene            7120..7413
                     /locus_tag="PLH85_14510"
     CDS             7120..7413
                     /locus_tag="PLH85_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNB65827.1"
                     /translation="MKTTSDRSGRLLRIHGLVQGVGYRNALLAEARRLGLDGWVRNRT
                     DGTVEALAEGPTTAVNTLIEWARRGPPAARVSGVDAIPADSAAEPGFRRLPTA"
     gene            7630..10116
                     /locus_tag="PLH85_14515"
     CDS             7630..10116
                     /locus_tag="PLH85_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65828.1"
                     /translation="MTAQPPSASEEREFPLFLFEHLPTLIWRSGPDSQCSYFNQTWLD
                     FTGRSLAEEIGDGWTLGVHPEDFDRCVGTYLQHFAARTPFEMEYRLRRHDGEYRWILD
                     TGRPIVTPSGEFAGYIGSCLDITDRRNTEAALKERENRMTTLLASMGEGVIMRDAGGD
                     ILMHNAAAERILGLTASDLARWNVFSPFLAYVNEEGVPVEPAQLPAMQAIASGEPRSG
                     VIGIDRPDGQRRWLLTNCVPLFNNGEKQASAVVTTIADITERRLHEEQLRLAWTVFRN
                     SVEAILVTDASHRIISVNKAFCEVTGYSEAEAIGATPRLLKSGLHDRPFYDAIWAAID
                     TAGFWQGEIYDRRKDGSLYPAAMSISAVHDRSGRLTHYVSVFSDITERKASEARIAYL
                     AQHDALTGLPNRALLHDRLEQALAAAARHDRRLAVLFLDLDRFKTINDSLGHLIGDRL
                     LKGVAERISACIRETDTVSRQGGDEFLIVLTDFNIPDDAARVAEKILDRITAPFDLDG
                     HQLGTSFSIGIALYPDDGTTPETLMKNADTAMYHAKESGRNTYRFFDEAMNANALERL
                     HLENALRRAQEHAEFELHYQPQVDLVTGRIIGLEALLRWFSPTLGNVSPVRFVPLAEE
                     CGLIVPIGEWVLHEACRQARAWQDLGLPAVPVAVNLSALQFRRNDIVATVSQALAESR
                     LAGAWLELELTESLLMQSGPDIVQILKQLKALGVRMSIDDFGTGYSSLAYLKRFPVDQ
                     LKIDRSFVRDLTEDPDDAAIVRAVVQLGRSLRLDVIAEGTETPEQLDFLRNEGCAAAQ
                     GYVFSPPLPPAAITELLRIGVLPAAPLMPM"
     gene            complement(10103..10582)
                     /locus_tag="PLH85_14520"
     CDS             complement(10103..10582)
                     /locus_tag="PLH85_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNB65829.1"
                     /translation="MSASFLDADLLDGLVTQARSVPRRRAHHNLHTDLAAPAQRLLVA
                     MEPDSFVRPHLHLDPHKAETLVILRGRLGVLLFAPDGSVIGTRLLVPGGACLGYDVAP
                     GCLHAVIALEPGSVFLEAKAGPYIPLAAAEIPAWSPAEDSPEAAGCLRRWAALFTSA"
     gene            complement(10579..>12120)
                     /gene="dnaE"
                     /locus_tag="PLH85_14525"
     CDS             complement(10579..>12120)
                     /gene="dnaE"
                     /locus_tag="PLH85_14525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238306.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time; main
                     replicative polymerase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNB65830.1"
                     /translation="GSGMVDDFILRKKGQQAIDYFHPDLKACLEPTYGVIVYQEQVMQ
                     ISQIIGGYTLGGADMLRRAMGKKKPEEMAKHRETIAAGARQQGYDPALAEQLFDLMTK
                     FAEYGFNKSHTAAYAVVTYHTAWLKAYHCAAFMAATMSSDLDNTDTVKIFYEDTLANG
                     ITVLPPDVNCSDYRFVPTDARTLRYGLGAVKGVGEPAVRAILAARSEGGPFKDLFDFC
                     QRVDKRLANRRVIEALIRAGAFDAIAPDQPAERARLLASVGIAMEAAEQIAANALQGG
                     LFDFADEPAAPMVDYVSVRPWGEREKLKEEKLAIGFFLSGHPFNAFRDEVRRFVRRPL
                     SQVEPGKELIMLAGVVMEVRAKMTARGKIAFVLLDDGTMPREVSVFSEVFDANRQRIV
                     VDEVLVVEAKVSNDDFSGGLRIVADRLMTLGEARGRFARALQIRLNGEVAEAGGAAAA
                     AERLQTTLAPFREGECPIRVRYRNAEAEADFPFGSGWRVRPEDGLLDSLREWLPPEAV
                     EVVYG"
BASE COUNT         1988 a         4387 c         3749 g         1996 t
ORIGIN      
        1 cgcagcatgt tctcgccgaa gaactggtgg aacatctgcc cgaccgggct cttcaggaag
       61 gccacgccgc cggaatggcc ggggcagtgc cacgaatagg agccgtcgga ggcgtaatgg
      121 gtgagcgccc ggaagaaagg cggcggcagc gcatccatgt aggcgcgggc ctcgcgcacc
      181 acgttgcggg cgatgaactc cggcgtgtcc tcgtacatgt ggatgaagcc gtggagctct
      241 cgcagcacat cgttggggat gtgccgcgac gtgcgcgtct cgccgtgcag gaagatgggg
      301 atgtccgcat tgcgcaggcg gatctcgctg acgaaggcgc gcaggtccgc gatggccttg
      361 gcagcggcct ccggcgacga gaactcctcg tcgtcgatgg acaggatgaa ggtggaggcg
      421 cggctctgct gctgggcgaa ggacgccagg tcgccgtagc tggtcacgcc caccacctgc
      481 aggccttcct tctcgatggc ttccgccagg gcgcggatgc ccagcccgga cgtgttttcc
      541 gaacgaaagt cctcgtcgat gacgatgacc ggaaaattga agcgcatgct cgactcctcg
      601 atgtccgccg cgcggacggc tcaacaaaaa aggcgtgggc ggatctgcgt ggctgcgcgc
      661 aggatccgcc ccttatcgga ctgtagccga cccgtgtttc agattttggg cagggtgacg
      721 ccggtctgcc ccaggtattt gccaccgcgg tcacggtaag acgtgtcaca gatctcgtcc
      781 gactcgaaga acagcatctg ggcgacgccc tcgttggcgt agatcttggc gggcagcggc
      841 gtggtgttgg aaaactccag ggtcacgtgg ccttcccatt ccggctccag cggcgtgacg
      901 ttcacgatga tgccgcagcg ggcgtaggtg gacttgccca ggcacacggt gagcacgctg
      961 cgggggatgc ggaagtactc gacggtgcgg gccagggcga aggagttcgg cggaatgatg
     1021 cacacgtcgc cggtgaaatc cacgaagttg cgctcgtcga aggccttggg atcgacgatg
     1081 gtggagttga tgttcgtgaa gatcttgaat tcgttggcca cgcgcacgtc gtagccatag
     1141 ctggaggtgc cgtaggagac gatcttgccg cctgcgttct ggcgcaccag ttcgggggcg
     1201 aagggttcga tcatcttcgc atcgccttcc gccatgcgac gaatccactt gtccgacttg
     1261 atgctcacgc tggctgcccc cgcacaaaaa cgcgcattct acatcccttc gtctggaaaa
     1321 ccggccaaaa aatcggggcc ttacggcccc tcgacccgcc cgccgcacgg gcccgcatca
     1381 gcgtccggcg tccttgacgt cgtcgtcctt tacggcatcg tagacgccct tgcccacctt
     1441 gaagggcacc ttgaccacct cgacgcccac atctaccgcc gtgccgacca cggtgccgac
     1501 aacacagccc gacagactgg ccgccaggac gacggccggt ccccacgcac gcaggcgccg
     1561 catcaggtgt tctgcaccac gatgctgggg aacttgtgac tcatgtcctt ctgccgctcg
     1621 gccacggcaa tcgccacctt gcgggcaatc tccttgtaga cctgcgcgac gcgcccatcc
     1681 ggatcggccg ccacggtcgg cgtaccgccg tccgcctcgc gacggatctg gatgtccagc
     1741 ggcagggcgc cgaggaaggg cacgccatag tcctcaccca tcgtcgcacc gccgcccgaa
     1801 ccgaagatgt gctcttcgtg gccgcactgg gagcagatgt ggatgctcat gttctcgacg
     1861 atgccgagga tcggcacgcc caccttctcg aacatcttga ggcccttgcg ggcgtccagc
     1921 agggcgatgt cctggggcgt ggtgacgatc accgcgccgg tcaccggcac gctctgggag
     1981 agggtcagct ggatgtcgcc ggtgcccggc ggcatatcaa tgaccaggta gtccagatcg
     2041 cgccagttgg tctccttgag gagctggttg agggcctggg tggccatcgg gccacgccac
     2101 accatgggcg tctccacgtc caccaggaag ccgatggaca tcacctgcag gccgtgggcc
     2161 tccagcggct ccatggtctt gccgtcgcgg gattccggct gggaatcgcc gatgcccagc
     2221 atctggggct gggacgggcc gtagatgtcc gcgtcgagga tgcccaccgt ggcgccttca
     2281 gccgcgaggg ccagggccag gttcgcggac gtggtgctct tgcccacgcc gcccttgccg
     2341 gaggccacgg caatgatgtt cttcacgccg ggcaacagct tcacgccctg ctgcacggcg
     2401 tgggcgacga tccgggtgaa tacattggcg ctcacattgc ccacgcccgg cacggacttc
     2461 gcacaggcga tcaccgcctg gcggatcgtg tcgatctggc tcgcggcggg atagcccagt
     2521 tcgatatcga aggacacgtc ggcaccgtgc aggcgcaggt tcttgacggc gcgggacgtc
     2581 acatagtcct tgccggtgtt ggggtcggcg tactgccgga ggctgtcgag aatgctctgt
     2641 tctgtgaggc tcatgggcgc gcaggccgcg ttgcggcgat tcggaaaggg tggatgatgc
     2701 gggcacgcct ccccatccgg aggcgcaaac ccgagtgatt tagtccagtt tacccaaaag
     2761 cgggccggtc cgcgatgggc tcgtcgcttg gtagaatgca ggattcgatc caccgatcca
     2821 ggcctgtttc atgaccacgc gcaagatcct cgtcaccaac gccctgcctt acgccaacgg
     2881 cgacatccac ctcggccacc tcgtcggcta cattcaggcc gacatctggg tgcgctacca
     2941 gcgcatgcgc ggccacacgg tgcactacgt ctgtgccgac gacacccacg gcacgccggt
     3001 catgctgcgg gccgaaaagg aaggcctcac gccggagcag ctcatcgccc gcgtccatgg
     3061 cgaacacctg cgcgacttca ccgacttcgg cgtggccttc gaccactatc attccaccca
     3121 cagcagcgag aaccgctggt acgcggaaga catctacacc cgcctgcggg acagcgcgcg
     3181 cctcattgag acccgcgcca tcgaacagtt ctacgacccg gtcaaggaga tgttcctgcc
     3241 cgaccgcttc atcaagggcg aatgtcccaa gtgcggcgcc caggaccagt acggcgacaa
     3301 ctgcgaggta tgcggcgctg cgtatgcgcc caccgatctc aagcaccccc gctccgccgt
     3361 gtccggcgcc gaacctgtgc tcaagacctc cgagcactat ttctttcgcc tatccgatcc
     3421 ccgcgcggtg gaattcctgc gggaatggac ccagggcacc acacccgaag gcgaacgccg
     3481 cctgcaggcc gaggcggcca acaagatgaa ggagtggctc ggcgactccg gcgagaacgc
     3541 gctctccgac tgggacatct cccgcgacgc accctacttc ggcttcgaga tccccggcgc
     3601 gccgggcaag tacttctacg tgtggctgga cgcccccatc ggctacttcg ccagcttccg
     3661 tcacctggcc atcctgcgtg gcgacatcga cgttgccgac tacaccgaag ccggccccgc
     3721 agccgccacc ggcaccgaac tgatccactt catcggcaag gacatcctct acttccacgc
     3781 cctcttctgg cccgccatgc tccacttcgc cggctaccgc acgcccaccc agttgtgcgt
     3841 gaacggtttc ctcaccgtcg atggcgccaa gatgagcaag agccgcggca ccttcatcac
     3901 cgcccgttcc tacaccgaac agaagctcaa ccccgagtgg ctgcgctact actttgccgg
     3961 caagtccaac ggcaccatgg aagacgtgga cctgaacctg gacgacatga tcgccaaggt
     4021 caattccgat ctggtaggca agttcgtcaa tatcgccagc cgctgcgcgg gcttcatcgc
     4081 caagcgcttc gacggcaagc tcgccgccca cgacagcgcc ctcgacctgg cctggagcgc
     4141 cgacgaggtg gcggctgcct acgaggcccg tgactacagc cgcgccctgc gccgcatcat
     4201 ggaattcgcc gaccaggcca atgcctacgt aaatgaccac aagccctggg agctcgccaa
     4261 gcaggacggc caggcggcgc gcctgcacgt cgtgtgctcc accgccctca acctcttcaa
     4321 ggccctcagc ggactgctca agccggtgct cccggccctc gccagccagg tggagacctt
     4381 cctgcggatc gagcctctcc agtgggccga tgtcctcaca ccacttccgg ccggccatgc
     4441 catcctgagc tacagccacc tcatgacccg tatcgagcgc aagcagatcg atgcgctcct
     4501 cgacgccaac cgggaatccc tcgcccccgc ggcggccacg ccggccaagg cagacaagac
     4561 cacccagccc gcccactcgg agcagcgcca cgcccagcat caggcccacg cggccaaggt
     4621 cgaagcggcc catgcctcgg ccttcgagcc cttcattggc atcgacgact tcgccaaggt
     4681 ggacctgcgc atcgcccgca tcgtctccgc cgagcacgtg gacggcgccg ccaagctgct
     4741 gcgcctccag ctcgacattg gcgaggcgca gccacgtcag gtcttcgccg gcatcaagtc
     4801 cgcttacgac ccggccaccc tggtcggccg gctcaccgtg atggtggcca acctcgcccc
     4861 gcgcaagatg aagttcggaa tgtcagaagg catggtgctc gccgcctccg acgagtcggg
     4921 cacccatgcg gggctgttca tcctgtcgcc ggacgccggc gccgtgcccg gcatgcgcgt
     4981 caagtaaggc cggcgtaagc ctgccgtcag cctcttctcg cacagtgccc gcccatgact
     5041 gaacgctccg tccccaccct gcgcctgccg cccgccttct ttggctgggc gctggtggat
     5101 ctcatcggcg tcgccgtgat ggcagtgggc gccgcctggc tcatcgaggg ccgcgcatcc
     5161 ctactgcctg gcttcccggc caacaccctg caggcctgga tctgcgtggt cggcggtctg
     5221 ggcaccctgt tcgtggccgc cgtcaagatg ctggtggaag tgatgcacgc caacaagcgc
     5281 ggtggcgccg ccaacgacga cgccttcggc cgctagagct tccctcacca tgcccctcac
     5341 cgcctgcaaa cgatggttcg tccagcatcg taccgggccg gggccgaagc ctccgcacgt
     5401 ggcgccaccc ggcgccttcg ttccaatcga ccgattgcgg aggttccatg aagaagatct
     5461 cgttccaccc caagctcctt gacaccctgc ccggctacga tgccggccag ttcggccgcg
     5521 acctgtccgc cggcctcacc gtgggcgtcc tcgccctgcc cctggccatg gccttcgcca
     5581 tcgcctcggg catgtccccc accgccggta tctggacggc catcgtcgcc ggcttcctga
     5641 tctccgcgct gggcggctcg cgggtgcaga tcggtggtcc caccggggcc ttcatcccca
     5701 tcctgtacgg catcgtcgtc gagtacggcg ttcagaacct gctggtggcc accatgctgg
     5761 ccggcgccat gctggtactg atgggcgtgt tccgtgttgg caacgtgatc cgtttcatcc
     5821 ccaagaccgt ggtgatcggc ttcaccaacg gcatcgcggt ggtgatcttc ctcgcccaga
     5881 tcaaggactt cctcggcctg cgcatcgagc acctgccggg cgagttcttc ggcaaggtgc
     5941 atgccctgtt cacgcacctg gacaccctcg acgtgcctac cctggccctg tccgtcgcct
     6001 cgctggcggt actgctgtcg tggaacggcg tggcccggcg ctaccctgca ctggggcgcg
     6061 tccccggtcc cctcggggtc ctcgtggtgg cgaccgtggc caatgccatc ctgcacctcc
     6121 cggtggaaac catcggcagc cgctttggcg gcattcccca ggaaattccg cccctcgcgc
     6181 tgcccgacct gacgctcgcc accctgggca agctgatttc cccggccctc accatcgccc
     6241 tgctgggcgc catcgagtcg ctcctgtcgg cgcgggtcgc cgacgccacc atcgaggatc
     6301 gccacgaccc caaccaggaa ctcatcggcc agggcatcgc caatctgatt gtgcccctct
     6361 tcggcggatt cgccgccacc ggcgccatcg cccgcacggc caccaacgtg cgtaccggcg
     6421 gccgcacgcc cgtggccggc atgctccacg ccggcgtgct gctcgccatc gtgctggccc
     6481 tggcgccctt tgcccgccac atcccgctgg ccaccctgtc ggccatcgtc gtggtggtgg
     6541 cggtcaacat gggcgaatgg cacgagttcc gcggcctcgg ccgcttctcg gtgaattacc
     6601 gcaccaccct gctgtccacc ttcctcgtca ccgtggtctt cgacctgacc ctcgccgtgg
     6661 aactgggcat ggtgctggcg agcctgttct tcatctaccg catgtcggac ctgacccggg
     6721 tcgagcggga gcccctgccg gagctcacct tgctgcccca cttcctgtac ccggacggct
     6781 cgccgcgggt cgtggcctac gccctctacg gctccctgtt cttcggcgcc atccacaagc
     6841 tggagaccct cctcgacccc gccgagcgcc acccggaggt ggtgatcctc gacatgctgc
     6901 acctggtcaa tctggacacc accggcctcg aaggtctcga aagcctgtgc aagaatctcc
     6961 accgcaaggg ctgcgcgctc cttctgtgca ccctcaatcc gcagcccgaa tccctcatcc
     7021 gccgctcggg cttcgccgag gaggtgggcg tcaacaatct ctgcgccgac atgtacgcgg
     7081 ccctcgcccg cgccaaggtg ctgctgccga gcttcatgga tgaagacaac ctctgaccgt
     7141 agcggccgcc tgctgcgcat ccacggccta gtgcagggcg tgggctatcg caacgccctg
     7201 ctcgccgagg cccgccgcct cggcctcgac ggctgggtgc gcaaccgcac cgacggcacc
     7261 gtcgaagccc tcgccgaggg tccgaccacc gccgtgaaca ccctcatcga gtgggcccgg
     7321 cgcggcccgc cggccgcgcg ggtgagcggc gtggacgcga taccggccga cagcgcggcg
     7381 gaacccggct tccggcgcct cccgaccgcc tgacagccgg cctaaacgtc cggtggcccc
     7441 tggtcacggc ggcatccccc gacgccaccg cgtcacaccg cacctctgcg ggcaccgcgc
     7501 ctgaaccttt gaaccggaca accctgaaca attcagggcc ttcggagtct cacgggtgcg
     7561 cgtctaaaga tgtgccacgt gacctccgtt gacgtgcaca ccccggcttc aatccctacc
     7621 cccgcccacg tgaccgccca accgccctcc gcgtccgaag aacgggaatt ccccctgttc
     7681 ctcttcgagc atctgcccac cctgatctgg cggtccgggc cagactccca gtgctcgtat
     7741 ttcaaccaga cgtggctgga tttcaccggt cgcagtctgg ccgaagagat cggtgacggc
     7801 tggacgctgg gcgtacaccc ggaagacttc gaccgctgcg tcggcactta cctgcagcat
     7861 ttcgcggcgc ggacgccgtt cgagatggaa taccggctgc gtcgccatga cggggaatac
     7921 cgctggatcc tcgacaccgg ccggcccatc gtcaccccct ccggggaatt cgccggctac
     7981 atcggctcct gcctggacat caccgaccgc cgcaacaccg aggccgccct caaggagcgg
     8041 gaaaaccgca tgacaaccct gctcgcctcc atgggcgaag gcgtcatcat gcgcgacgca
     8101 ggcggcgaca tcctcatgca caacgccgcg gccgagcgca tcctcggcct cacggcgtcc
     8161 gacctggccc gctggaacgt attctcgcct ttccttgcct acgtgaacga ggagggcgtg
     8221 ccggtcgagc ctgcccagct gcctgccatg caggccatcg ccagcggcga gccccgctcc
     8281 ggcgtgatcg gcatcgaccg ccccgacggg cagcggcgct ggctgctcac caattgcgtg
     8341 cccctcttca acaacggcga aaagcaggcc agcgcggtgg tcaccaccat cgccgacatc
     8401 accgaacggc gcctccacga ggaacagctc cgcctggcgt ggacggtgtt ccgcaacagc
     8461 gtggaagcga ttctcgtgac cgacgccagt caccgcatca tctccgtgaa caaggccttc
     8521 tgcgaggtga ccggctattc cgaggccgag gccatcggcg cgacgccgcg cctcctcaag
     8581 tccggcctgc acgaccgccc gttctacgac gcgatctggg cggccatcga cacggccggc
     8641 ttctggcagg gcgagatcta cgaccggcgc aaggacggca gcctctaccc ggccgccatg
     8701 tccatcagcg ccgtgcatga ccgcagcggc cgcctgaccc attacgtgtc ggtgttttcc
     8761 gacatcaccg aacgcaaggc ctcggaggcg cgcatcgcct acctcgccca gcacgacgcc
     8821 ctcacgggcc tgcccaaccg ggccctgctc cacgaccgtc tggaacaggc cctcgcggcg
     8881 gccgcccgcc acgaccgtcg tctggccgtg ctgttcctcg acctggaccg cttcaagacc
     8941 atcaacgatt ccctcggcca tctcatcggt gaccgtctgc tcaagggtgt ggccgagcgg
     9001 atcagcgcct gcatccgcga gaccgatacg gtgagccgcc agggcggcga cgagttcctg
     9061 atcgtcctca ccgatttcaa catccccgac gacgccgccc gcgtggcgga aaagatcctc
     9121 gaccgcatca cggcgccctt cgatctggac ggccaccagc tcggcacctc cttctccatc
     9181 ggcatcgccc tctatccgga cgacggcacc accccggaaa ccctgatgaa gaacgccgac
     9241 acggccatgt accacgccaa ggagagcggc cggaacacct accgcttctt cgacgaggcc
     9301 atgaacgcca acgcgctgga gcgcctacac ctcgagaacg ccctgcgccg ggcccaggag
     9361 cacgcggaat tcgagctgca ctaccagccc caggtggacc tggtgaccgg ccgcatcatc
     9421 ggcctggaag ccctgctgcg ctggttcagc ccgaccctcg gcaacgtgtc gcccgtgcgc
     9481 ttcgtgccgc tggccgagga atgcggcctg atcgtgccca tcggcgaatg ggtcctccac
     9541 gaagcctgcc gccaggcccg cgcctggcag gacctgggac tgcccgccgt gccggtggcc
     9601 gtcaacctgt cggcccttca gttccgtcgc aacgacatcg tcgccacggt ctcccaggcg
     9661 ctggccgaaa gccgtctggc cggcgcctgg ctcgaactgg agctgaccga gtccctgctg
     9721 atgcagtccg gcccggacat cgtgcagatc ctcaagcagc tcaaggcgct gggcgtgcgc
     9781 atgtccatcg acgacttcgg cacgggctac tccagcctgg cctatctcaa gcgctttccg
     9841 gtggatcagc tcaagatcga ccgcagcttc gtgcgcgacc tgacggaaga tccggacgac
     9901 gcggccattg tgcgggccgt ggtgcagctc ggccgcagcc tgcgtctgga cgtgatcgcg
     9961 gagggtaccg aaacccccga gcagctggac ttcctgcgca acgaaggctg cgccgcggca
    10021 cagggctatg tgttcagccc gcccctgccg ccagccgcca tcacggagct gctgcgcatc
    10081 ggcgtactgc ccgccgcccc cctcatgccg atgtgaacaa ggccgcccag cgacgcagac
    10141 acccggcggc ctcagggctg tcttctgccg gcgaccaggc gggaatctcg gcggccgcca
    10201 gcggtatgta ggggcccgct ttcgcttcca ggaagacgct gcccggctcc agcgcgatca
    10261 cggcgtgaag gcagcccggc gccacgtcat accccaggca ggcgcccccg ggcaccagca
    10321 ggcgcgtccc gatcacggac ccgtctggcg cgaacagcag cacccccagc cgcccgcgca
    10381 ggatgaccag cgtttcggcc ttgtgcggat caaggtgcag gtgcggccgt acgaaggagt
    10441 ccggctccat cgccaccagc aggcgctggg cgggggcggc gaggtccgtg tggaggttat
    10501 ggtgcgcccg ccttcgcggg acgctgcggg cttgcgtcac cagcccgtcc aggagatccg
    10561 catcgagaaa gctcgcactc atccgtacac cacctccact gcctcgggcg gcaaccattc
    10621 ccgcaggctg tccagcaggc cgtcctcggg ccgcacgcgc caacccgatc cgaacgggaa
    10681 atcggcctcc gcctcggcgt tacggtagcg cacgcggatc gggcattcgc cctcgcggaa
    10741 gggcgccagc gtggtctgca ggcgctcggc cgcagctgct gcgccgccgg cctcggcgac
    10801 ctcgccattg aggcggatct gcaaggcacg ggcaaaacgg ccgcgggcct cgccgagggt
    10861 catcaggcgg tcggcgacga tgcgcaggcc gccggagaaa tcgtcattgc tcaccttggc
    10921 ctccaccacc agcacttcgt ccaccacgat gcgctggcgg ttggcgtcga agacttccga
    10981 gaacaccgac acctcccgcg gcatcgtgcc gtcgtccagc agcacgaagg cgatcttgcc
    11041 gcgggcggtc atcttggcgc gtacctccat gaccacgccg gccagcatga tcagttcctt
    11101 gcccggctcg acctgggaca agggccgccg cacgaagcgc cgcacctcgt cccggaaggc
    11161 attgaagggg tggccggaca ggaagaaacc gatggcgagc ttctcttcct tgagcttctc
    11221 ccgctcgccc cagggtcgca ccgacacgta atcgaccatc ggcgccgcgg gctcgtcggc
    11281 aaaatcgaac aggccgccct gcagggcatt ggcggcgatc tgctcggcgg cctccatggc
    11341 gatgcccacc gaggcgagca ggcgggcgcg ctcggcgggc tggtccggcg cgatggcatc
    11401 gaaagcgcca gcgcggatca gggcctcgat gacgcgccgg ttggccaggc gcttgtccac
    11461 ccgctgacag aagtcgaaca gatccttgaa cggtccgccc tcgctgcgcg ccgcgaggat
    11521 ggcccgcacc gccggctcgc ccacgccctt gaccgcgccc agaccgtagc ggagggtcct
    11581 ggcgtcggtg ggcacgaagc ggtagtccga acaattgacg tccggcggca gcacggtgat
    11641 gccgttggcg agggtgtcct cgtagaagat cttgaccgtg tccgtgttgt ccaggtcgga
    11701 ggacatggtc gccgccatga aggccgcgca gtggtaggcc ttgagccagg cggtgtgata
    11761 ggtgacgacg gcgtaggcgg cggtgtggga cttgttgaag ccatactccg cgaacttggt
    11821 catcaggtcg aagagctgct cggccagggc cggatcgtaa ccctgctggc gggccccggc
    11881 ggcgatggtc tcccggtgct tggccatctc ctcgggcttc ttcttgccca tcgcccggcg
    11941 cagcatgtcg gcgccgccca gcgtgtagcc cccgatgatc tgggagatct gcatcacctg
    12001 ttcctggtac acgatgacgc cgtaggtggg ctccaggcag gccttgaggt ccgggtggaa
    12061 gtaatcgatg gcctgctggc ccttcttgcg caggatgaag tcgtccacca tgcccgagcc
//
LOCUS       DAOQMM010000109        12107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2340_length_12107_cov_42.239379, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000109 DAOQMM010000000
VERSION     DAOQMM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2340_length_12107_cov_42.239379"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..828
                     /locus_tag="PLH85_14530"
     CDS             352..828
                     /locus_tag="PLH85_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB65831.1"
                     /translation="MTVKRMDNVGIVVEDIDGAVEFFAELGLTLEGRMPIEDEWAGRV
                     TGMRGQRVEIAMMSTPDGHSRIELSRFDAPAIASDHRSAPVNSLGYMRVMFAVDDLDD
                     TLTRLSKLGATVVDEVVNYENIYRLCYIRGPEGILIGLAQQLGQQTSRENSMDRKP"
     gene            complement(1031..1609)
                     /locus_tag="PLH85_14535"
     CDS             complement(1031..1609)
                     /locus_tag="PLH85_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65832.1"
                     /translation="MSFDTVFRTQGEPPTLHYKALVLAKGQERPLEVWRDHDRRLKRV
                     TGDDLEVYATKSPGQTEFSLSILDKKKRIHTRIDRSNLYRIGNFADWFDLAHGLRHPA
                     GPHRIAQSNAPKDAPQAAVPCTWYAITQDPRTTHVCWSTDLRIPALIVAEDGRGLWKL
                     VAADKKPIPAGTFEIHDEGFVRNDANRDIESD"
     gene            2031..3689
                     /locus_tag="PLH85_14540"
     CDS             2031..3689
                     /locus_tag="PLH85_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HNB65833.1"
                     /translation="MPVRLIAPTPSAYAFPLLIKHLLLTPLATVPEQTITYQGRVRYT
                     YRTLKDRIGRLANVLAGLGVEAGQTVAVMDWDSHRYLEAFFAVPMMGAILQTVNVRFS
                     PEQILYTLNHARADVLIVNSEFFAALGEIAGRLETVKTVVTIVEDSKAAAAPLAAVGE
                     YEALLAGASADYAFPDFDENAQATTFYTTGTTGHPKGVHFSHRQLVLHTLGCAAALGG
                     ATTQGRVHREDVYMPLTPMFHVHAWGFPYLATMMGLKQVYPGRYQPELICRLVAEEKV
                     SFSHCVPTILHMVMGHPLAKTTDFAGWKILIGGSALPEAMATAAQKRGIDLFTGYGMS
                     ETCPVLTIAHLDSAALAGSAESQSAIRAKTGRPLPLVDLRVVDPQMRDMLHDGKATGE
                     VVVRAPWLTQAYVNAPEASEALWAGGYLHTADIGHIDAAGYLKVTDRIKDVIKTGGEW
                     TSSLELENLIGKHEAVHEVAVIGVPDEKWGERPLALVLLKPEHADTITERALRNWAAH
                     AIEAAGVSRYGVLLQVRFVKTLVKTSVGKMNKRLMRADPDALCL"
     gene            4062..4577
                     /locus_tag="PLH85_14545"
     CDS             4062..4577
                     /locus_tag="PLH85_14545"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNB65834.1"
                     /translation="MRSLRLFVALLLCCATSAFAAPASEETIRQILSVTNARALAESV
                     LTQMDANISQGINQSLQGKQPNPAQQQAIERFKARMVTTLRDALAWDKLEAMNIRLYK
                     EAFSEEELKGILAFYKTPAGQAVIHKMPVLMAQTMREMQQTMVSVTPALLKIQQEFGE
                     EMKAAAETSSQ"
     gene            complement(4739..5062)
                     /locus_tag="PLH85_14550"
     CDS             complement(4739..5062)
                     /locus_tag="PLH85_14550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNB65835.1"
                     /translation="MKRGELYWVASFKDFGKVRPAVVVQADAFNENPTSVTVCLLTGE
                     RVDAPLFRLDVPADTETGLDKPSQIMADKLITVPVARIKEPIGKIPARTLSKLDDSLR
                     LGRSL"
     gene            complement(5059..5280)
                     /locus_tag="PLH85_14555"
     CDS             complement(5059..5280)
                     /locus_tag="PLH85_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65836.1"
                     /translation="MKTVSVTDLRRKPIETALAIAAAVQGGQGLEITRRGRVVEVIGA
                     DRPGKDEVARQLDVMRRADATDDFADFAE"
     gene            complement(5494..5841)
                     /locus_tag="PLH85_14560"
     CDS             complement(5494..5841)
                     /locus_tag="PLH85_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB65837.1"
                     /translation="MPTDPAAAMNLEGVEACHWQDVPPRPLEHPGFSTRLLWRGPDGR
                     RALIFEIAPGAVYPELDVHLPGAEEVFVVSGSFCDGRNTYPAGTFIHHPAGSSHVPQS
                     EEGCVLFVYFPEG"
     gene            complement(5978..7618)
                     /gene="gpmI"
                     /locus_tag="PLH85_14565"
     CDS             complement(5978..7618)
                     /gene="gpmI"
                     /locus_tag="PLH85_14565"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNB65838.1"
                     /translation="MLQKLPAFPGVAGPVVVIVLDGYGLPKSDVGSAIAAARKPTLDR
                     LFAECPHIRLRAHGTAVGMPSDDDMGNSEVGHNAIGAGQVYAQGAALVSNAIADGAIW
                     QGEAWQQIIAGARAGRGVLHFIGLFSDGNVHSHIDHLKAMVIRAKAEGIPTVRIHALL
                     DGRDVPETSALDYVVPFEAFLAELSSGGFDARIASGGGRQAITMDRYDANWPMVARGW
                     RTHVLGEGPQFANATAAVHGLRERHPGTIDQDLPEFVIADAGQPIGTIEDGDAVVFYN
                     FRGDRAIEITRAFVDESFDKFDRVRTPQVTYAGMLQYDGDLQLPKRFLVAPPAIKDTS
                     GEWFSRLGLTQFACSETQKFGHVTYFWNGNRSGKFEGETWQEVPSDVVPFEQRPWMKA
                     AEITDAMIAAVQSGQYALLRCNYANGDMVGHTGNFRAATMAVEAVDLALARLLPAIQA
                     AGGVALITADHGNADEMFELDKKTKQPALNTDGSYKAKTAHTLNPVPLVLFDKVTGGR
                     LGLQQTDTAGLSNIAATVANLLGYAKHPAWDDSVLTID"
     gene            complement(7822..8241)
                     /locus_tag="PLH85_14570"
     CDS             complement(7822..8241)
                     /locus_tag="PLH85_14570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNB65839.1"
                     /translation="MNCVERVVFDTSTLIGAMLRPTSIPRQAFLKAVRQAELCASPAT
                     LAELEVVLLRDKFDRYLGREHRLQFLELYRRHARLFAVSDADETEMPCRDPRDNKFLA
                     LALACSANAIVSSDDDLQVLNPFREIPILLPRDYLDR"
     gene            complement(8228..8503)
                     /locus_tag="PLH85_14575"
     CDS             complement(8228..8503)
                     /locus_tag="PLH85_14575"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNB65840.1"
                     /translation="MRTLTSVEAQNRFGELIDSAQREPVSITRRGRTVAFVLSPADMQ
                     ALLAVRQQRERVVADFEAFFSESDARLDPQADPLSDADIQRLVNELR"
     gene            complement(8582..9403)
                     /locus_tag="PLH85_14580"
     CDS             complement(8582..9403)
                     /locus_tag="PLH85_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HNB65841.1"
                     /translation="MPPAAPAALAARPLFEPLRGLLEAFRDPVLPDAAALTALLHAHA
                     PGAVSGSGRPIRFVLPPPGDTAGYEAHIHATGEVPTRPDDWHDFFNALAWAVWPRTKS
                     ACNALHLQEIARRQAAGQAGRGPVRDALTQLDECGVIVVTCDPALPARLADHQWDTVF
                     VTHRAQLATTTRFLVLGHATWDQLRAPFVGLCGKALFRCVEPAWFALDPFAQQAECDA
                     WLAESLVARAASLTPRAWSPLPLLGIPGVTPESEDPAYYRDTRQFRAKRRATSCC"
     gene            complement(9403..9843)
                     /locus_tag="PLH85_14585"
     CDS             complement(9403..9843)
                     /locus_tag="PLH85_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65842.1"
                     /translation="MRLRALFLLAAVPFLAACENEGIAYLIDGKDHSIALMREQKWFW
                     SDEVDQAIVVSRMPTCLRRYDIKSGVAGSVKMEVYEAGDYLWALKQGKNWYLAGTEKC
                     EFQRWKDAPETPPGKLVGTFTRKDGRLQFVPADAAPKPIKPAEE"
     gene            complement(9858..10892)
                     /gene="pyrC"
                     /locus_tag="PLH85_14590"
     CDS             complement(9858..10892)
                     /gene="pyrC"
                     /locus_tag="PLH85_14590"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB65843.1"
                     /translation="MSRLTLTRPDDWHLHVRDGGALAAVVPDTARRFGRALIMPNLKP
                     PVTSVAQAAAYRERILAAVPAGLKFAPLMTLYLTDTLPPDEIDRAAASGFIPAAKLYP
                     AGATTNSDAGVTDIAKVYPVLERMAEAGIVLCVHGEVTHADVDIFDRERVFIDRVLAP
                     LATRFPSLRIVFEHITTADAAQFVTDAGPNVGATITAHHLLLNRNAIFTGGIRPHHYC
                     LPVLKRETHREALVKVATSGSAKFFLGTDSAPHARSTKEAACGCAGCYTAHAGIELYA
                     EAFEQAGALDRLEAFASFHGPDFYRLPRNADTITLVKDAWDVPAALDYLPGDPLVPLR
                     GGEQIGWKLA"
     gene            11375..11938
                     /locus_tag="PLH85_14595"
     CDS             11375..11938
                     /locus_tag="PLH85_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNB65844.1"
                     /translation="MTMSTTPEQFVALNKANVETLLTLANTAFASAERLAALNLNTAR
                     AVLEDSVANAKSLLSAKDVQELVNLQAALLQPVVEKAVAYSRSVYEIASQTQEEVSKL
                     FEAQVAEANKNLAAALDKAAKSAPAGSDVAVAAVKSAIAAANSAYDSVTKAAKQVAEI
                     AEANVAAATSATVKAVSAPAKSSKKAA"
BASE COUNT         2025 a         3699 c         4354 g         2029 t
ORIGIN      
        1 acgttagcgt cttcttggta accccgaatg aaactaccac ccacagccga tgaacaaatg
       61 aagtgtttct tcattccagg gccgttgctc ttggcttttg ctgggttcag cggccgtttc
      121 gttccctcca actatctgtg ggtttctgtg ctcgtagcgg ctatctcagt tccattcgca
      181 ctcatgttca tcgcagccag cttccgcaca ctcaggcaat cacggaggga aaagtagtga
      241 aagccattcg tcgtcgccgc gccgccgcta acatcccgct ccagcggacc gtcggccagc
      301 ttcgctgccc gccgtccgct gagctagcac gttagctgcc taggagacat cgtgacagtc
      361 aagcgtatgg acaacgtggg catcgtggtt gaagacattg atggggccgt tgagttcttt
      421 gctgaactcg gccttactct cgaaggccgc atgcccatcg aagacgagtg ggccggccgc
      481 gtaaccggaa tgcgcggtca gcgcgtcgag attgccatga tgagcacccc cgacggccac
      541 agccgcatcg agctttcgcg cttcgacgcc cccgccattg cgtccgatca ccgttcagca
      601 ccagtgaact ctttgggata tatgcgcgtc atgttcgccg ttgatgatct cgacgatacc
      661 ctcacccgac ttagtaagct cggtgcaacg gtggtcgatg aagttgtcaa ttacgaaaac
      721 atttaccggc tctgttacat ccgggggcct gaaggcattc tcattggtct cgcccaacaa
      781 ctcgggcagc aaacgtcccg agagaattcc atggatcgta aaccatgagc attcaggcat
      841 tgtcggctaa cccggcagtt gagcggacct gcgcgaaaag ccgcgcaggc cggtgaattc
      901 aaacgttgaa cgacagcttt cccaagagct ttacggctgc tctggatcgg tagcggacat
      961 cagcaatcac atctcccaag ccccgccttc cgccagacag ccctgaatcc ccgtcagggc
     1021 catcccctcc tcaatcactc tcaatatccc gattcgcatc gttccgcacg aacccttcat
     1081 catgaatctc gaaggtgccg gcgggaatcg gcttcttatc cgccgcgacg agcttccaca
     1141 gcccccgccc atcctccgca acgatcagtg cggggatgcg gaggtcggtg ctccagcaga
     1201 cgtgggtggt gcgcgggtcc tgggtgatgg cgtaccaggt gcacggcacg gcggcctggg
     1261 gcgcatcttt gggtgcgttg ctctgggcga tgcggtgggg gccggcgggg tggcgtaagc
     1321 cgtgggcgag gtcgaaccag tcggcaaagt tgccgatgcg gtagaggttg ctgcggtcga
     1381 tgcgggtgtg gatgcgcttt ttcttgtcga ggatcgaaag gctgaattcg gtctggccgg
     1441 gtgacttggt ggcgtagacc tccaggtcgt cgccggtgac gcgtttgagg cggcggtcgt
     1501 ggtcgcgcca gacttcgagg gggcgttcct ggcctttggc gaggacgagg gccttgtagt
     1561 gcagggtggg cggttcgccc tgggtgcgga agacggtgtc gaagctcaaa tctttggccg
     1621 cggcagaaaa ggaaactgcg acggccaggg cgggcatggc gcgtcgcagt gtccatcccc
     1681 cgcggcgacg ggggatggga tgcatcaggt gaaatctccg ggaatccgtt gcggacggcc
     1741 ttggcggagt cgaagtagcg gtaggtcttg ccgtcgacgg tgaggtcttt gaccttgagg
     1801 ttgatggtgt aggtgccatt gccggcggtg aaggccgggt cggcggtggc gccgggggga
     1861 ttgttgagcg tgtcaagggt gagaccgtca cgcttgagtg attgcttgaa tgcgccgtca
     1921 caaggcccca cggcggttgg cagacagcat cacttcgcct tggtacacac cggtccggtc
     1981 gtcacgcagg cgtaaaatat ccgcccgaat cccgcccgcc ggagcccctc atgcccgtcc
     2041 gcctcatcgc ccccaccccg tccgcctacg cgtttccgct gctgatcaag catctgctcc
     2101 tcacgccgct ggcgacggtg ccggagcaga cgatcaccta tcaggggcgg gtgcgttaca
     2161 cctaccgcac gctcaaggac cgcatcggca ggctggcgaa tgtgctggcc gggctggggg
     2221 tggaggccgg gcagacggtg gcggtgatgg actgggacag tcaccgttat ctggaagcct
     2281 tcttcgcggt gccgatgatg ggggcgatcc tgcagacggt gaatgtgcgc ttcagcccgg
     2341 agcagatcct gtacaccctc aaccacgccc gcgcggacgt gctgatcgtg aacagcgagt
     2401 tctttgcggc cctcggcgag atcgcggggc ggctggagac ggtgaagacg gtggtgacca
     2461 tcgtggagga cagcaaggcg gcggccgcgc cccttgcggc ggtcggcgaa tacgaggcgc
     2521 tgctggcggg ggcctctgcg gactacgctt tccccgattt cgacgagaac gcccaggcca
     2581 ccacctttta tacgacgggg acgacgggcc atccgaaggg cgtgcacttc agccatcgcc
     2641 agctggtgct gcacacgctg ggctgtgcgg cggcgctggg cggggccacg acccaggggc
     2701 gggtgcatcg ggaggatgtg tacatgccgc tgacgcccat gttccacgtg cacgcctggg
     2761 gctttccgta tctggcgacg atgatggggc tgaagcaggt gtatccgggg cgctatcagc
     2821 cggagctgat ctgccggctg gtggcggagg agaaggtgag cttttcccac tgcgtgccga
     2881 ccatcctgca catggtgatg ggccatccgc tggcgaagac gacggatttt gcgggctgga
     2941 agatcctgat cggcggttcg gccctgccgg aggcgatggc gacggcggcg cagaagcgcg
     3001 gcatcgacct ctttaccggc tacggcatgt cggagacctg tccggtgctg acgattgccc
     3061 atctggacag cgcggcgctg gccggctcgg cggaatccca gtccgcaatc cgcgccaaga
     3121 cggggcggcc gctgcccctg gtggatctgc gcgtggtgga cccgcagatg cgcgacatgc
     3181 tccacgacgg caaggccacc ggcgaggtgg tggtgcgcgc gccgtggctg acccaggctt
     3241 atgtgaatgc gccggaggcc tccgaggccc tgtgggcggg cggctatctg cacacggcgg
     3301 acatcggcca catcgacgcc gccggttacc tgaaggtaac cgaccgcatc aaggacgtga
     3361 tcaagaccgg cggcgaatgg acctcgtccc tggagctgga gaacctgatc ggcaagcacg
     3421 aggcggtgca cgaggtggcg gtgatcggcg tgccggacga gaaatggggc gagcgccccc
     3481 tcgccctggt gctgctcaag cccgagcacg cggacacgat caccgagcgg gccctgcgca
     3541 actgggcggc ccacgccatc gaggccgcgg gggtgtcgcg ctacggcgtg ctgctgcagg
     3601 tgcgcttcgt gaagaccctg gtgaagacca gcgtgggcaa gatgaacaag cgcctgatgc
     3661 gcgccgatcc ggacgccttg tgtctgtaat gggacgcggc cagagtgcgg cggtgccacg
     3721 gcactgaagg atgcgcggcc gggcgggaag gattcgacgt cggcggtgaa ggcgcccgcc
     3781 gcagcggaga aggctccggc ggcgtccaca ctgcactcgg gcggctgcgc gaggcattcc
     3841 gccgggcgtg aatcgcccgc gcagcgacgc gggaaggatt gtgcgccctt cgccgcggcg
     3901 tgcacggcgt gcgtcccccc ggcgtgcccc ccgcgcagga caggcggccg gccctatgcc
     3961 agaatgctgc ctcacccgac cccgccgccg cgccggcttt gtttgcgccg cccggcgatg
     4021 gacctgcgat ccgttccacg cctgcctccg aggtttctcc catgcgttcc ctccgcctgt
     4081 ttgtcgccct gctgttgtgc tgcgccacct ccgccttcgc cgcgcccgcc tccgaggaga
     4141 cgatccgcca gatcctgtcg gtgaccaacg cccgcgccct ggcggaatcg gtgctgaccc
     4201 agatggatgc caacatcagc cagggcatca accagtccct ccagggcaaa cagcccaacc
     4261 cggcccagca gcaggccatc gagcgcttca aggcgcgcat ggtgacgacc ctgcgcgacg
     4321 cactggcctg ggacaagctg gaggcgatga acatccgcct ctacaaggaa gccttcagcg
     4381 aagaggaact gaagggcatc ctcgccttct acaagacccc cgccggccag gcggtgatcc
     4441 acaagatgcc agtgctgatg gcccagacca tgcgcgaaat gcagcagacc atggtctccg
     4501 tgacccccgc actgctgaag atccagcagg agttcgggga ggagatgaag gcggcggcgg
     4561 agacgtcttc ccagtgagcg gaggctggcc tgtttttggt gtgcaggtct cttccgcgca
     4621 gatctgagac gcgggccaag gcagcgcggg ccctgatcgc cagcggcccc cattggcaga
     4681 ggcgtctacg acaaggcttt cacgtgcaga acatgtccgg gcatcgcgcc cggattcttt
     4741 aaagggaccg cccgaggcgg agtgaatcgt ccaatttgga cagggtgcgt gcgggaatct
     4801 tgccgatggg ttccttgatg cgggcgaccg gcacggtgat cagtttgtcc gccatgatct
     4861 gcgacggctt gtcgaggccg gtttccgtgt cggccggtac gtcgagtcga aacagcggcg
     4921 catccacccg ttctccggtc agcaggcaga cagtcacgct ggtgggattt tcgttgaagg
     4981 catcggcctg gaccacgacg gccggacgga ccttgccgaa gtccttgaag ctggccaccc
     5041 agtagagttc gccgcgcttc attcggcaaa gtcggcgaag tcgtcagtgg cgtcggcccg
     5101 gcgcatcacg tcaagctggc gcgccacttc atccttcccc ggccggtcgg cgccgatgac
     5161 ctccaccacg cgcccccgac gggtgatttc cagcccctgt ccgccctgca ctgcggccgc
     5221 aatggccagg gcggtttcga tgggtttgcg gcgcaagtcg gtgacagaga cggtcttcat
     5281 gggctttccc cttccgctga cggaatgatg caccccacgt gccgggatgc gcgcgcccgg
     5341 aaggacatgc ccccgtctgc ggggcgttcg ccgccatcgg atgcgccggg gcggcttgca
     5401 ccaacccgtc gcgggggatc gaaaggggcg cgcgcagcgg gaggcgttcc ggtcgggcgc
     5461 catgacggcg gccccgcgac cgataacgcc gtctcaacct tccggaaaat acacgaacag
     5521 cacgcagcct tcctccgact ggggcacgtg ggacgagccg gccgggtgat ggatgaaggt
     5581 gccggcggga taggtgttgc ggccgtcgca gaagctgccg gagacgacga agacttcctc
     5641 ggcgccgggc agatgcacgt ccagctcggg atagacggcg ccgggggcga tctcgaagat
     5701 cagcgcccgc cgcccgtccg ggccgcgcca cagcagacgg gtgctgaagc cggggtgttc
     5761 cagtgggcgc ggggggacgt cctgccagtg gcaggcttcg acgccttcga ggttcatcgc
     5821 ggcggcagga tcggtgggca ttggctctct caggcaggat tgaaagacac gcggcgcgcg
     5881 gtgaggtgga tcaccccgtc gcggaacgcc gcggcggtgg ccgcgacgcc cacagagggc
     5941 gggcgccgcg caggccgccc gcgcatacca ccgcggatca gtcgatggtg aggacgctgt
     6001 cgtcccaggc gggatgcttg gcgtagccga ggaggttggc gacggtggcg gcgatgttgg
     6061 agaggccggc ggtgtccgtc tgctgcaggc cgaggcggcc gcctgtgacc ttgtcgaaga
     6121 ggacgagggg cacggggttc agcgtgtggg cggtcttggc cttgtaggag ccgtcggtgt
     6181 tgagggcggg ctgtttggtc tttttgtcca gctcgaacat ctcgtcggcg ttgccgtggt
     6241 cggcggtgat gagggcgaca ccgcccgcag cctggatggc ggggagcagg cgcgcgaggg
     6301 cgagatccac ggcctcgacg gccatggtgg cggcgcggaa gttgccggtg tggccgacca
     6361 tgtcgccgtt ggcgtagttg cagcggagca gtgcgtactg gccggactgg accgcggcga
     6421 tcatggcgtc ggtgatttcg gcggccttca tccacgggcg ctgctcgaag ggcaccacgt
     6481 cgctggggac ttcctgccag gtttcgcctt cgaacttgcc ggagcggttg ccgttccaga
     6541 agtaggtgac gtggccgaac ttctgggtct cggagcaggc gaactgggtg aggccgaggc
     6601 ggctgaacca ttcaccggag gtgtccttga tggcgggcgg ggcgacgagg aagcgcttgg
     6661 gcagttgcag gtcgccgtcg tactggagca tgccggcgta ggtgacctgc ggggtgcgca
     6721 cgcggtcgaa cttgtcgaag ctttcgtcga cgaaggcgcg ggtgatctcg atggcccggt
     6781 cgccgcggaa gttgtagaag acgacggcgt cgccgtcttc gatggtgccg atgggctggc
     6841 cggcgtcggc gatgacgaac tcgggcaggt cctggtcgat ggtgccggga tggcgttcgc
     6901 gcaggccgtg gacggcggcg gtggcgttgg cgaactgggg cccttcgccg aggacgtggg
     6961 tgcgccagcc gcgggccacc atgggccagt tggcgtcgta gcggtccatg gtgatggcct
     7021 gacggccgcc gccggaggcg atgcgggcgt cgaaaccgcc gctgctgagc tcggcgagga
     7081 aggcttcgaa gggcacgacg tagtccagcg cgctggtttc gggcacgtcg cggccatcga
     7141 gcagggcgtg gatgcgcacg gtggggatgc cttctgcctt ggcgcggatc accatggcct
     7201 tgaggtggtc gatgtggctg tgcacgttgc cgtcggagaa caggccgatg aagtggagca
     7261 cgccgcggcc ggccctggcg ccggcgatga tctgctgcca ggcttcgccc tgccagatgg
     7321 cgccgtcggc gatggcattg gagacgagcg cagcgccctg ggcatacacc tggccggcgc
     7381 cgatggcgtt gtgacccact tcggagttgc ccatgtcgtc gtcggagggc atgccgaccg
     7441 cggtgccgtg ggcgcgcagg cggatgtgcg gacattcggc gaagaggcgg tcgagggtgg
     7501 gcttgcgcgc ggcggcgatg gcgctgccca cgtcggattt gggcaggccg tagccgtcga
     7561 ggacgatgac gacgaccgga ccggcgacgc cggggaaggc ggggagtttc tgcagcatgg
     7621 agggcttcct gaaggatgaa cgcgaggccg gtccggcggg gcgcatcgcc cacggccccc
     7681 ggcgagggcg ctattgtcct tcaaatgagg gggtgaagac aggtttatgt caggtggttc
     7741 gtgacgggtg gccggggtgg gggggcgctc atcgcgccct cgcgcgcgac accattcaag
     7801 tgcgagcgcg gcgaacccgc gtcatcggtc cagatagtcc cgggggagca ggatggggat
     7861 ctcgcggaag gggttcagaa cctgcaaatc gtcatcgctg ctgacaattg cgttggcaga
     7921 acacgccagc gcaagcgcga ggaatttgtt gtcacgcgga tcgcgacagg gcatttcggt
     7981 ctcatcggcg tccgatacgg cgaacagtcg ggcgtgacga cggtagagct ccaggaattg
     8041 caggcgatgc tcgcggccga ggtaacggtc gaacttgtcc cgcaaaagca cgacttcgag
     8101 ttcggccagg gttgcgggcg aggcgcagag ttcggcctga cggacggcct tcagaaacgc
     8161 ctgacgaggg atggaggtgg gccgcagcat cgcgccgatc agcgtgctgg tgtcgaaaac
     8221 gacccgctca acgcagttca ttcaccaacc gctgaatatc cgcgtcggaa agcggatcgg
     8281 cctgagggtc gaggcgggca tccgattcac tgaaaaaagc ttcgaaatcc gccacgacgc
     8341 gctcacgctg ctgacggacg gcaagtaagg cctgcatgtc ggcgggcgag agcacaaagg
     8401 ccaccgtccg gccacggcgg gtaatgctga ccggctcgcg ttgggcgctg tcgatcaact
     8461 caccgaaacg attctgggct tcgaccgaag tgagtgtccg catgggaatg ctcctcgctg
     8521 aaatagctaa cctgtccact atagcccacc cctgcggatg ctcgatccgt tagcgctcgt
     8581 actagcagca ggatgtcgcc cggcgcttcg ccctgaactg ccgcgtatcc cggtagtacg
     8641 ccggatcttc gctctcgggc gtgacgccgg ggatacccag cagcggcagg ggcgaccagg
     8701 cgcggggcgt gagggatgcg gcgcgggcga cgagggattc ggcgagccag gcatcgcatt
     8761 cggcctgctg cgcgaaagga tcgagggcga accaggcggg ttcgacacag cggaacaggg
     8821 ccttgccgca caggccgacg aagggcgcgc ggagctggtc ccaggtggcg tggccgagga
     8881 cgaggaagcg ggtggtggtg gcgagctggg cgcggtgggt gacgaagacg gtgtcccatt
     8941 gatggtcggc gaggcgcgcg ggcagcgccg ggtcgcaggt gacgacgatc acaccgcatt
     9001 cgtcgagctg ggtgagggcg tcgcggacgg ggccgcggcc ggcttggccg gcagcctgac
     9061 ggcgggcgat ttcctgcagg tgcagggcgt tgcaggcgga cttggtgcgc ggccacacgg
     9121 cccaggcgag ggcgttgaag aagtcgtgcc agtcgtccgg gcgggtcggg acttcgccgg
     9181 tggcgtggat gtgggcttcg tagccggcgg tgtcacccgg cggcggcagg acgaagcgga
     9241 tcggccggcc gctgccgctg acggcgcccg gcgcgtgagc gtgcagcagg gcggtgaggg
     9301 ctgcagcgtc gggcagaacg ggatcgcgga aggcttcgag caggccgcgc aggggctcga
     9361 acaggggacg cgccgccagg gcggccggcg cggcgggcgg gatcactctt cggcgggctt
     9421 gatgggcttg ggcgcggcgt cggcgggcac gaactggaga cggccgtcct tgcgcgtgaa
     9481 ggtgccgacg agtttgccgg gcggcgtctc gggggcgtcc ttccagcgct ggaactcgca
     9541 cttctcggtg ccggcgaggt accagttctt gccctgcttg agggcccaca ggtagtcgcc
     9601 cgcttcgtac acttccatct tgacgctgcc ggcgacgccg gacttgatgt cgtagcggcg
     9661 caggcaggtg ggcatgcgcg agacgacgat ggcctgatcc acctcgtcgc tccagaacca
     9721 cttctgttcg cgcatcaggg cgatggaatg gtccttgccg tcgatgaggt aggcgatgcc
     9781 ctcgttctcg caggcagcga ggaagggcac ggcggcgagg aggaacaggg cacgcaggcg
     9841 catcgtggtg gcggatgtca ggcgagcttc cagccgatct gctcgccccc gcgcaggggc
     9901 acgaggggat cgccgggcag gtagtcgagg gcggcgggca cgtcccaggc gtccttgacc
     9961 agggtgatcg tgtcggcgtt gcggggcagg cggtagaaat ccgggccgtg gaagctggcg
    10021 aaggcttcca gtcgatccag cgcgccggcc tgctcgaagg cttcggcgta gagctcgatg
    10081 ccggcgtggg cggtgtagca gccggcgcag ccgcaggcgg cttccttggt gctgcgggcg
    10141 tggggcgcgg agtcggtgcc gaggaagaac ttggcgctgc cggaggtggc gaccttcacg
    10201 agggcctcgc gatgggtttc gcgcttgagc accggcaggc agtagtgatg gggccggatg
    10261 ccgccggtga agatcgcgtt gcggttgagc agcagatggt gggcggtgat cgtggcgccc
    10321 acgttgggcc cggcgtccgt cacgaactgc gccgcatcgg ccgtcgtgat gtgctcgaag
    10381 acgatgcgca gggacgggaa gcgcgttgca agcggcgcca gcacgcgatc gataaagacg
    10441 cgctcgcgat caaagatatc cacgtcagca tgggtcacct cgccatgaac acagagaacg
    10501 atgcccgctt cggccatccg ttcgaggacg ggatagacct tggcgatgtc ggtgacgccc
    10561 gcgtccgagt tggtggtggc gccggccggg tagagcttgg cggcggggat gaagccgctc
    10621 gcggcagccc ggtcgatctc gtcgggcgga agggtgtcgg tgaggtacaa cgtcatcagc
    10681 ggggcgaact tgagccccgc gggcacggcc gcgaggatgc gttcgcgata cgcggcggcc
    10741 tgcgccacgc tcgtcaccgg cggcttgagg ttgggcatga tcagcgcgcg gccgaagcgg
    10801 cgcgcggtgt cgggcacgac ggccgcgagg gcgccgccgt cgcgcacatg aagatgccag
    10861 tcgtcggggc gggtgagggt caggcgggac atgggaaaga tccgggctgg ggaatgacgg
    10921 gaatgcgcat tttaacagcg cccccacccc gcggaggcgc caccggcggg gcgaagaccc
    10981 tgaaaaacgg ctgcgccgcc gatcggaagg cgcgggatca ggtgccagat gcatcgattt
    11041 ctcctggcgg catggcaggg tggtccggac ggtccaaagc ggtgcagcgc gggcgtcgcg
    11101 ggggtctccg agcctgcttc cggaagcccc cgaaacgccc gaaatcggcg tgcctgctgc
    11161 atttcgggcg actcacgcct gctatccgga tggcacctcg tcagcgtgtt aaccccattg
    11221 ttttgaaacg gaaatacagg ccgcaaatat ttttgtgcgg cgcaacaaaa aagtgttgac
    11281 agcactcttt cggtgcatat aatggaaccc atgttgcagc gcaataaagc aacgccccga
    11341 cacagaacgt aatcaccctt tttaccttta ggagatcacc atgagcacca ctcccgagca
    11401 attcgtcgcc ctcaacaagg ccaacgtcga aaccctgctg accctggcca acaccgcctt
    11461 cgccagcgcc gagcgtctcg ccgccctgaa cctgaacacc gcccgcgccg tgctggaaga
    11521 cagcgttgcc aacgccaagt ccctgctgtc cgccaaggac gtgcaagagc tggtgaacct
    11581 gcaagccgcc ctgctgcagc ccgtcgtgga aaaggccgtg gcctactccc gcagcgtcta
    11641 cgagatcgct tcccagaccc aggaagaagt ctccaagctg ttcgaagccc aagtcgctga
    11701 agccaacaag aacctggctg ccgccctcga caaggctgcc aagtccgctc ccgccggttc
    11761 cgacgtcgcc gtggctgccg tgaagtccgc catcgccgcc gccaactccg cctacgacag
    11821 cgtgaccaag gccgccaagc aagtcgccga aatcgccgaa gccaacgttg ccgctgccac
    11881 cagcgccacc gtcaaggccg tcagcgcccc ggccaagtct tccaagaagg ctgcctaagt
    11941 ttcacccgga tccggatcgc gctccggcgc ggtcttgatc gcgacatggc cccaccggca
    12001 acggtgcggg ccatgtttgt tttggggccg cgcccgacgc actggcccga acgaaaacgc
    12061 ccccgacatg cgggggcgtt ttcgtgccgg gatcggcgcg gggctga
//
LOCUS       DAOQMM010000110        12038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2363_length_12038_cov_41.202787, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000110 DAOQMM010000000
VERSION     DAOQMM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2363_length_12038_cov_41.202787"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /gene="prfA"
                     /locus_tag="PLH85_14600"
     CDS             <1..224
                     /gene="prfA"
                     /locus_tag="PLH85_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988158.1"
                     /note="recognizes the termination signals UAG and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF1; this protein is
                     similar to release factor 2; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNB65845.1"
                     /translation="AREAATRKSLVGSGDRSERIRTYNFPQGRLTDHRINLTLYKLDA
                     VMQGEMDDVIAALTAEHQAELLAALADEA"
     gene            224..1084
                     /gene="prmC"
                     /locus_tag="PLH85_14605"
     CDS             224..1084
                     /gene="prmC"
                     /locus_tag="PLH85_14605"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNB65846.1"
                     /translation="MDAPATLGAALVAARGRIPQSEARLFLRAIAGVSAAQLAAYPER
                     DLTPAQAARFADWLARRTAGEPVAYLLGEREFYGRCFRVTPATLIPRPETELIVDWAK
                     TRLAPDAPARVLDLGTGSGALAVTLALEWPAAAVTGIDRSPAALAVAAANGAALGASV
                     RWLESDWCAALEPAERFDLIVSNPPYVAADDPHLAEGDVRFEPRSALASGPRGLDDIA
                     RIAVTAPPFLVPGGWLIVEHGYDQAEAVRALLAEQGLGAVDSARDLAGIERISFGRCP
                     DGLDALRRGA"
     gene            1129..1440
                     /gene="grxD"
                     /locus_tag="PLH85_14610"
     CDS             1129..1440
                     /gene="grxD"
                     /locus_tag="PLH85_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNB65847.1"
                     /translation="MDVQQIIHEQVTTHPVVLYMKGTPAMPRCGFSSLAAQILNACGV
                     KEFFSVDVLADEAIRQGIKEYANWPTIPQLYIKGEFVGGSDIMREMYESGELKQMLAA
                     D"
     gene            complement(1516..2796)
                     /locus_tag="PLH85_14615"
     CDS             complement(1516..2796)
                     /locus_tag="PLH85_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB65848.1"
                     /translation="MERIGKYEIRRLLGEGSTSAVYLGFDPFTQRDVAIKRLHPEVTR
                     NSSRATLYRQLLLNEAVLAGQLHHPHIAQIHDAVIDEDDAYVVMEYVPGGTLEPFTDP
                     ARLLPFERLIEIIFKCTRALDYAHLQGITHRDVKPANILLTSPDGQDIKVSDFGAALF
                     TASETTQLAGLGSPAYMSPQQVRELPVDHRTDIYSLGVVMFQLLTGRLPFEATSNVNL
                     IYQIAHHVPPPPSSVRPEVPPELDAIVGRATQRDLADRYATWAEFSHDLAEAYRNRDL
                     AVSPREPRDSEKFERLRAFRFFREFSDVEIWEIVRFAEWHLYPPGTVVMKEGEPGNHF
                     CFVVQGTLRVVKGGHLLNVLTPGDCFGEMALFSTGRGARTASVEAEDATRVLTIRAPS
                     LLRASSECQMHFYKGFLEVLAARLTLANRRITGP"
     gene            complement(2808..4262)
                     /gene="argH"
                     /locus_tag="PLH85_14620"
     CDS             complement(2808..4262)
                     /gene="argH"
                     /locus_tag="PLH85_14620"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNB65849.1"
                     /translation="MADQSAPQDGAAKAWSGRFSEPVSDLVKRYTASVFFDNRMAAQD
                     IRGSLAHARMLARQGIIGAQDLADIERGMAQIAGEIERGEFAWNLDDEDVHLNIEKRL
                     TALVGDAGKRLHTGRSRNDQVATDIRLWLRDAIDTVLGLIREFQLAVLDLAEHHAATP
                     LPGFTHLQVAQPVTFGHHLMAYFEMTRRDAERFADCRTRVNRLPLGSAALAGTTFPID
                     REFVAAQLGFDEVCFNSLDAVSDRDFAIEFCAASALLMTHLSRLSEELILWMSPRVGF
                     IDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGSLIALLTLMKGQPLAYNKDNQED
                     KEPLFDTADTVIDTLRIYADMMGSRVEAGQKIFNVRVKPEAMRAALSQGYATATDLAD
                     YLVKKGLPFRDAHEAVALAVRAAEARGCDLPQIPLADLRAAMAHVPGAVERMGDDVFQ
                     VLTVEGSLAARNHVGGTAPEQVRAAIARARARLG"
     gene            4351..5367
                     /locus_tag="PLH85_14625"
     CDS             4351..5367
                     /locus_tag="PLH85_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB65850.1"
                     /translation="MSIKQNPGRTGRRHDGPLPDFRNLGVVLRVLVGVNGLGLIAVLA
                     RSGQWSSLGGALMDMAMCVEPPLIVAVAALFLLQPVLARMPAPAGWVGVGAVALAAAA
                     LFNTLLAPLDVGDLASWLGWTALLVVAVRAYFRYLAAVYAPSLAEARLLALTARIRPH
                     FLFNSLNGVLGILRSDPRRAEAALEELADLFRALMRENRDLVPLADEIALCERYLALE
                     HLRLGPRLSVRWDCAPDVGTAQVPPLFLQPLVENAVYHGIEPGAGPGEVAIAIRRHGP
                     ELRIEIVNPLQGGHSHPGGNRMAVDNIRERLMLFFDLEAGLETEQADGLHRVRIRLPY
                     RTAT"
     gene            5364..6149
                     /locus_tag="PLH85_14630"
     CDS             5364..6149
                     /locus_tag="PLH85_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB65851.1"
                     /translation="MKEHTPPAPDTPLSVLIVDDEAPARERLRDLLGDIAERHPTRLV
                     GMAANGIEALALLDTTPADLILTDIRMPAMDGIELARHTARLDPPPAVIFTTAYDEYA
                     VRAFELAAVDYLLKPVRAARLAEALAKARRLHPPADTVLRQIATAGRSHFSVSERGRI
                     LLVPVPEVLYLKAEQKYVVARCVDREYLLDESLVQLEEEFPERFLRIHRNCLIARHAV
                     RGVARATTSEDGEAHWVILLDGLPDQLPVSRRQWPTVKDALGV"
     gene            6292..8406
                     /locus_tag="PLH85_14635"
     CDS             6292..8406
                     /locus_tag="PLH85_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNB65852.1"
                     /translation="MSRAEMDALGWDACDVILVTGDAYIDHPSFGMALVGRALEAQGF
                     RVGIISQPDWHSAEPFRALGRPRLFFGVTAGNMDSMINRYTADRKPRSDDAYTPDALP
                     DKRPDRAVVVYAQRAREAFPEVPVVLGSIEASLRRIAHYDHWSEKVRRSVLLDSKADL
                     LVFGSGERAVIALAHRLAAGEAIADIRDLRGTAFMVKPGWRPAGFVEIDSGAIDRPGP
                     VEAHPDPYAMAPDAPAAPADGAQPVRIVPAAERVAARKAARAQQVIRLPDYEAVKDDR
                     VLYAHASRTFHLESNPGNARALVQGHGSGPGRRDVWLNPPPIPLSTAEMDWVFDRPFA
                     RRPHPAYGEARIPAWDMIRFSINIMRGCFGGCTFCSITEHEGRIIQSRSEASILREIE
                     EIRDKTPGFTGTISDLGGPTANMYRMACKDPQIESACRRLSCVFPDICDNLGTDHGPL
                     IRLYRKARAIRGVKRVLIGSGLRYDLAVRSPEYVRELVTHHVGGLLKIAPEHTEDAPL
                     AKMMKPGIGTYDRFKELFERFSQEAGKKQHLVPYFIAAHPGTRDEDMVNLALWLKANQ
                     FRLDQVQTFLPTPMALATTMYHSGKNPLRKVSATSEEVASPRSARQRRLHKALLRYHD
                     PEGWPLIREALRALGRSDLIGSSPRHLVPRDQPVVLGEGGGERRRTGKAANPVGARRA
                     PPPLRRGTAPSPVSGKPRGRRS"
     gene            8541..9287
                     /locus_tag="PLH85_14640"
     CDS             8541..9287
                     /locus_tag="PLH85_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB65853.1"
                     /translation="MDRILCITGGSRGIGAATARLAARQGYAVAVGYLGRRDAAEALV
                     AQIVANGGVARALQVDVALEASVEAFFAAVDRALGPLTALVNNAGVLDTQCRLEDMDA
                     ARLQRVFATNVTGSFLCAREAVRRMSTRHGGHGGAIVNLSSRAARLGSPGEYIDYAAS
                     KGAIDTLTVGLAKEVAAEGIRVNAVSPGLIATDIHAAGGEPGRVARLASAIPMQRGGE
                     PDEVARAILWLLSDDASYVTGANLDVAGGR"
     gene            complement(9433..10482)
                     /locus_tag="PLH85_14645"
     CDS             complement(9433..10482)
                     /locus_tag="PLH85_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB65854.1"
                     /translation="MHATPDTPLRLGQTHGYRFDGDVVHLNAELQITNPAATAAGEWA
                     LQLWACDSVAPGALPAGIKVAELRCQPDTTFDTAWIDATVAALPPAGQGEHVMVLALV
                     SGHDGRFDTLCDTARFPRTERFVQPRLVGSTGFGLESNHVTVHVDRVDNPRDADNLSG
                     SLSLELWALSAPYTSDEPAGTRLATAALGVLGGGQSVDTLDFDLPLAARPAGTWHIAL
                     LLREWTGEAYATRDFSNFTLPVSFAAEPESPAAAAEAPAAQAIEAAEAEAAPAPAAKK
                     PAARKASAKSAAPASGVCINTASATELSAVKGLPKTVAAAIVAARPFARLDDLLAVKG
                     MGEKLLDKLRAALRV"
     gene            complement(10663..>12038)
                     /gene="rng"
                     /locus_tag="PLH85_14650"
     CDS             complement(10663..>12038)
                     /gene="rng"
                     /locus_tag="PLH85_14650"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNB65855.1"
                     /translation="HVERTASRGIVGNIYLGRVVRVLPGMQSAFMDVGLERTAFLHVA
                     DIWTDRHGGEAPRPIERILAEGQNLMVQVLKDPIGTKGARLSTQISIAGRMLVYLPQD
                     KHIGISQRIGDEAGREALRERVSRLLPKDESGGYIVRTMAENATDDELAADIAYLRKL
                     WAEIRNRTIGARPPYVLYQDLNLAQRVLRDLVTDATTRVVADSRENFQKLSAFTTEYM
                     PQVAERLEHYTGERPLFDLHGVEAEIEKALARRVDLKSGGYLIIDQTEAMTTIDVNTG
                     GFVGARNFDDTIFKTNLEAAQTIARQLRLRNLGGIIIVDFIDMESAEHRTAVLDEFRK
                     ALARDHTKMTVNGFTSLGLVEMTRKRTRESLAHLLCETCPTCGGRGEVKTARTVAYEI
                     LRELLREARQFNAREFRVLAAPQVIDLFLDEESQALGMLSDFIDKTVSLHPEASYGQE
                     QFDIVLL"
BASE COUNT         1723 a         4068 c         4336 g         1911 t
ORIGIN      
        1 aggcccgcga ggccgccacg cgcaagagcc tggtgggcag cggcgaccgc tccgaacgca
       61 tccgcaccta caacttcccc cagggccgcc tcaccgatca ccgcatcaac ctgaccctct
      121 acaagctcga cgcggtgatg cagggcgaga tggatgacgt catcgccgcc ctcaccgccg
      181 agcaccaggc cgagctcctg gccgcccttg cggacgaggc ctgatggacg cgccggcgac
      241 cctcggcgcg gcccttgtcg cggcccgcgg gcggattccc cagagcgagg cacgcctctt
      301 cctgcgggcc attgccggcg tgtcggcggc ccagctcgcc gcctatccgg agcgggacct
      361 gacgccggcg caggccgccc gcttcgccga ctggctggcc cgccgcacgg ccggcgagcc
      421 ggtggcctac ctgctgggcg agcgggaatt ctacggacgg tgcttccgcg tcacgcccgc
      481 cacgctgatt ccccgcccgg agaccgagct gattgtggac tgggccaaga cccggctggc
      541 gccggatgcg ccggcccgtg tcctcgacct gggcaccggc agcggtgctc tggcggtgac
      601 cctggcactg gagtggccgg ccgccgcggt gacgggcatc gaccgttcgc ccgccgcgct
      661 ggcggtggcg gcggccaacg gggcggccct cggcgcctcg gtgcgctggc tcgagagcga
      721 ctggtgcgcg gcgctggaac cggcagagcg tttcgacctg attgtcagca atccgcccta
      781 cgtggcggcc gacgatcccc atctggccga gggcgatgtg cgctttgaac cgcgctccgc
      841 actggcttcg ggcccccgcg ggctggacga cattgcacgc atcgccgtga ccgcgccgcc
      901 atttctggtg ccgggcggct ggctgatcgt cgagcacggc tatgaccagg cggaagcggt
      961 acgcgccttg cttgcggagc agggtctggg tgccgtggat tcggcgcgag atctggccgg
     1021 catcgagcgg atcagcttcg ggcggtgtcc ggacgggctt gacgccctgc ggcgaggggc
     1081 ctagactttt ccgactgaat tgctcaacta tttagcgaga acaccacgat ggacgtccaa
     1141 cagatcatcc acgaacaagt caccacccac ccggtcgtgc tgtacatgaa gggtacgccg
     1201 gccatgccac gctgcggttt ctcgtcgctg gccgcgcaga tcctgaacgc ctgcggcgtg
     1261 aaggaattct tctcggtgga cgtgctggcc gacgaggcca tccgccaggg catcaaggaa
     1321 tacgccaact ggccgaccat tccgcagctc tacatcaagg gcgaattcgt cggcggctcc
     1381 gacatcatgc gcgagatgta cgagtccggc gagctcaagc agatgctggc tgccgactga
     1441 gcgcccgaac agcatcccgt tcgctcaagc cgcccgcggg cggctttttt gcgggctgtg
     1501 atcggcgagc gccgctcagg gcccggtgat gcgccggttg gcgagggtca ggcgcgccgc
     1561 gaggacttcg aggaagccct tgtagaagtg catctggcac tcgctggagg cgcgcagcag
     1621 ggacggggcg cggatcgtca ggacgcgggt ggcgtcttcg gcttcgaccg aggccgtacg
     1681 ggccccgcgc ccggtggaga agagggccat ctcgccaaag cagtcgcccg gcgtgagcac
     1741 gttgaggagg tgtccgccct tcacgacgcg cagggtgccc tgcacgacga agcagaaatg
     1801 gttgcccggt tcgccttcct tcatgacgac ggtgcccggc gggtacaggt gccactcggc
     1861 gaagcgcacg atctcccaga tctccacgtc gctgaattcg cggaagaaac ggaaggcgcg
     1921 gaggcgttcg aacttttcgc tgtcccgcgg ctcccgcggg ctcacggcga gatcgcggtt
     1981 gcggtaggcc tcggccagat cgtgggagaa ctcggcccag gtggcgtagc ggtcggccag
     2041 gtcccgctgg gtggcgcgtc cgacgatggc gtccagctcg ggcggcacct ccgggcggac
     2101 gctcgacggc ggaggcggga cgtggtgggc gatctggtag atcagattga cgttggacgt
     2161 ggcctcgaag ggcagacggc cggtgaggag ctggaacatc accacgccga gggaatagat
     2221 gtcggtgcgg tgatccaccg gcagctcgcg tacctgctgg ggtgacatgt acgccggcga
     2281 accgaggccg gcgagctggg tggtttcgga ggcggtgaac agggccgcgc cgaagtccga
     2341 gaccttgatg tcctggccgt ccggcgaggt gagcaggatg ttggccggct tcacgtcccg
     2401 gtgggtgatg ccctgcaggt gggcgtagtc gagggcccgc gtgcacttga agatgatctc
     2461 gatcaggcgc tcgaagggca gcagccgggc aggatcggtg aagggctcca gggtgccgcc
     2521 gggcacgtac tccatcacca cgtaggcgtc gtcctcgtcg atgaccgcgt cgtggatctg
     2581 ggcgatgtgg ggatgatgga gctggcccgc cagcacggcc tcgttgagca ggagctggcg
     2641 gtacagggtg gcgcgcgacg aattgcgggt cacctcgggg tgcaggcgct tgatggccac
     2701 gtcccgctgg gtgaagggat cgaagccaag atagacggcg ctggtggagc cttctcccag
     2761 caggcgccgg atctcgtact tgccgatccg ctccatggcc gcgcgcctca gcccagacgc
     2821 gcgcgggcgc gagcgatggc ggcgcgcacc tgctccggcg ccgtgccgcc cacatgattg
     2881 cgggcggcga gggaaccttc cacggtgagg acctggaaca cgtcgtcgcc catgcgctcg
     2941 acggcgcccg gcacgtgggc catggcggcg cgcaggtcgg ccagcggaat ctggggcagg
     3001 tcgcagccgc gcgcctcggc ggcgcgtacg gcgagggcca cggcctcgtg ggcgtcgcgg
     3061 aagggcaggc ccttcttgac caggtagtcg gccaggtcgg tggcggtggc gtagccctgg
     3121 ctgagggcgg cgcgcatggc ctcgggcttg acgcgcacgt tgaagatctt ctggcccgct
     3181 tcgacgcggc tgcccatcat gtcggcgtag atgcgcaggg tgtcgatgac cgtgtcggcg
     3241 gtgtcgaaga ggggttcctt gtcttcctgg ttgtccttgt tgtaggccag gggctggccc
     3301 ttcatgaggg tcagcagggc gatcaggctg ccattgacgc ggccagtctt gccgcggacc
     3361 agttcgggca catccgggtt cttcttctgg ggcatgatgg agctgccggt gcagaagcgg
     3421 tcggccaggt cgatgaagcc gacccgcggg ctcatccaca ggatcagttc ttccgacaag
     3481 cgcgacaggt gcgtcatcag cagcgccgag gcggcgcaga attcgatggc gaagtcccgg
     3541 tcggagacgg cgtcgaggga gttgaagcac acttcgtcga agcccagctg cgcggcgacg
     3601 aattcccggt cgatggggaa ggtggtgccg gccagggcgg cggaacccag aggcaggcgg
     3661 ttgacccgcg tgcggcagtc ggcgaagcgc tcggcgtcgc ggcgggtcat ctcgaagtag
     3721 gccatcaggt gatggccgaa ggtgaccggc tgggcgacct gcaggtgggt gaagccgggc
     3781 agcggcgtgg cggcgtggtg ctcggccagg tcgaggacgg ccagctggaa ttcgcggatc
     3841 aggccgagga cggtgtcgat ggcgtcgcgc agccacaggc ggatgtcggt ggccacctgg
     3901 tcgttgcggc tgcggccggt gtgcaggcgc ttgccggcgt cgcccaccag ggcggtgagg
     3961 cgcttctcga tgttgaggtg gacgtcttcg tcgtccaggt tccaggcgaa ttcgccgcgc
     4021 tcgatctcac ccgcgatctg ggccatgccg cgctcgatgt cggccaggtc ctgggcgccg
     4081 atgatgccct ggcgggccag catgcgggcg tgggcgaggg agccgcggat gtcctgggcg
     4141 gccatccggt tgtcgaagaa cacgctcgcg gtgtagcgct tgaccagatc ggaaacgggt
     4201 tcggagaagc gcccggacca ggccttggcg gcgccgtcct gcggggcgga ttgatctgcc
     4261 ataattggaa tgctctcagc ggttcgaggg ggcgccctgc cgcggggatg cccgcagatt
     4321 caaggtcttg ccccggtttc tttttgattc atgagtataa agcaaaaccc cggccgcacc
     4381 ggccgccggc acgacgggcc gttgccggat ttccgcaatc tcggggtggt gctgcgcgtc
     4441 ctggtgggcg tgaatggcct cgggctgatc gccgtgctgg cccgcagcgg gcagtggtcg
     4501 agcctcggcg gagcgctcat ggacatggcc atgtgcgtcg agccgcccct gatcgtggcg
     4561 gtggccgccc tgttcctgct ccagccagtg ctggcgcgga tgccagcgcc ggcaggctgg
     4621 gtcggtgtcg gcgccgtggc cctggcggcc gcagccttgt tcaacaccct gctcgcgccg
     4681 ctggacgtgg gcgacctggc gagctggctg ggctggacgg cgttgctcgt ggtagccgtg
     4741 cgggcctact tccgttatct cgcggcggtc tatgccccgt ccctcgccga ggcgcgcctg
     4801 ctcgccctga cggcgcgcat ccggccccat ttcctgttca acagcctcaa cggggtgctc
     4861 ggcatcctgc gctccgatcc gcgccgggcc gaggcggccc tcgaggagct tgccgacctg
     4921 tttcgcgccc tcatgcgcga aaaccgcgat ctggtgccgc tggccgacga gatcgcgctg
     4981 tgcgagcgct atctggccct ggagcacctg cgcctcggcc cgcgcctgag cgtgcgctgg
     5041 gattgcgccc cggacgtcgg gacggcgcag gtgccgcccc tgttcctcca gcccctcgtc
     5101 gaaaacgccg tctatcacgg catcgagccg ggtgccggac cgggtgaggt cgccatcgcg
     5161 atccggcgtc acggtcccga actgcgcatc gagatcgtca atccgctgca gggtggtcac
     5221 agtcatcctg gcggcaaccg catggccgtg gataatatcc gcgagcgcct gatgctcttc
     5281 ttcgacctgg aagccggcct ggagaccgaa caggcggatg gcctccaccg cgtccgcatc
     5341 cgactgcctt accgcactgc gacatgaagg aacacacccc tcccgccccc gataccccgc
     5401 tctccgtcct catcgtggat gacgaggcgc ccgcccggga gcgcctgcgt gacctcctcg
     5461 gcgacatcgc cgagcgtcat ccgacgcgcc tcgtgggcat ggccgccaat ggcatcgagg
     5521 ccctggccct gctcgacacg acgccggccg acctgatcct caccgacatc cgcatgccgg
     5581 ccatggacgg catcgagctg gcccgccaca cggcccgcct cgatccgccg ccggcggtca
     5641 tcttcaccac cgcttacgac gaatacgcgg tgcgcgcttt cgagctggct gcggtggatt
     5701 acctcctcaa gccggtccgc gccgcgcggc tggccgaggc cctggccaag gcccgccgcc
     5761 tccatccgcc tgccgacacg gtgctgcgcc agatcgccac ggccgggcgc agccatttca
     5821 gcgtcagcga acgcggccgc atcctcctcg tgccggtgcc cgaggtgctc tacctcaagg
     5881 ccgagcagaa atacgtggtg gcccgctgcg tcgaccggga atacctgctg gacgaatccc
     5941 tcgtgcagct tgaggaagaa ttccccgagc gcttcctgcg catccaccgc aactgcctga
     6001 tcgcccgcca tgcggtgcgc ggcgtggccc gcgccaccac cagcgaggac ggtgaggccc
     6061 actgggtgat cctcctggac ggcctgcccg accagctccc ggtgagccga cgccagtggc
     6121 ccaccgtaaa ggacgccctc ggcgtgtgag ccgccgcggg tcgggaacgt ccgtggcggc
     6181 gggtgttcca acgggggctc ccgtacaatc gcgcccatgg aaaacacgcc ccgcctgctg
     6241 ccctcctatc cgcgccatcg gccggacctg cgtcccgcgc ccttcctgcc catgtcccgg
     6301 gccgagatgg acgccctcgg ctgggatgcc tgcgacgtca tcctcgtcac cggcgacgcc
     6361 tacatcgacc atcccagctt cggcatggcc ctggtcggcc gcgccctgga ggcccagggc
     6421 ttccgcgttg gcatcatcag ccagcccgac tggcattccg ccgagccctt ccgcgccctc
     6481 ggccggccgc gcctgttctt cggcgtcacc gccgggaaca tggactcgat gatcaaccgc
     6541 tacaccgccg accgcaaacc ccgctccgac gacgcctaca cccccgacgc cctgcccgac
     6601 aaacgcccgg accgggctgt ggtggtgtac gcccagcgcg cccgcgaagc ctttcccgag
     6661 gtgccggtgg tgctcggcag catcgaggcc agcctgcgcc gcatcgccca ttacgaccac
     6721 tggtccgaga aggtgcggcg ctcggtgctg ctggattcca aggccgacct gctggtgttc
     6781 ggttccggcg agcgggcggt gatcgccctg gcccaccgcc ttgccgccgg cgaggccatc
     6841 gccgacatcc gcgacctgcg tggcaccgcc ttcatggtca agccgggttg gcgtccggcg
     6901 ggcttcgtcg agatcgactc cggcgccatc gaccgccccg gtcccgtcga ggcccatccg
     6961 gacccttacg cgatggcgcc agacgcaccc gccgcgccgg cggatggcgc ccagccggtg
     7021 cgcatcgtcc ccgctgccga gcgggtcgcc gcgcgcaagg cggcgcgtgc ccagcaggtg
     7081 atccgcctgc ccgactacga ggccgtcaag gatgaccggg tgctctacgc ccacgcctcg
     7141 cgcaccttcc acctggaatc caaccccggc aacgcacgcg cgctggtgca gggccatggc
     7201 agcggaccgg gtcgtcgcga cgtgtggctc aatccgccgc ccattcccct cagcaccgcc
     7261 gagatggact gggtcttcga ccggcccttc gcccggcgcc cgcacccggc ctacggtgag
     7321 gcgcggattc ccgcctggga catgatccgc ttctcgatca acatcatgcg cggctgcttc
     7381 ggcggctgca ccttctgctc catcaccgag cacgaggggc gcatcatcca gagccgctcg
     7441 gaggcgtcca tcctgcggga gatcgaggag atccgcgaca agacgccggg cttcaccggc
     7501 accatctcgg atctgggcgg ccccaccgcc aacatgtacc gcatggcctg caaggacccg
     7561 cagatcgagt ccgcctgccg ccgcctgtcc tgcgtcttcc ccgacatctg cgacaacctg
     7621 ggcaccgacc acggcccgct gatccgcctg taccgcaagg cgcgggcgat ccgcggcgtc
     7681 aagcgggtgc tgatcggctc cggcctgcgc tacgacctgg cggtgcgctc gcccgagtac
     7741 gtcagggagc tggtcaccca ccacgtgggt ggcctgctca agatcgcgcc ggagcacacc
     7801 gaggacgcgc ccctcgccaa gatgatgaag cccggcatcg gcacctacga ccgcttcaag
     7861 gaactcttcg agcgcttctc ccaggaggcc ggcaagaagc agcacctggt gccctacttc
     7921 atcgccgccc atccgggcac gcgcgacgag gacatggtga acctcgccct gtggctcaag
     7981 gccaaccagt tccgtctcga ccaggtgcag accttcctgc ccacccccat ggccctggcg
     8041 accaccatgt accactcggg caagaacccg ctgcgcaagg tctcggccac gtcggaagag
     8101 gtggcctcgc cccgttccgc ccgtcagcgc cggctccaca aggccctgct gcgctaccac
     8161 gatccggaag gctggccctt gatccgcgag gccctgcggg ccctcgggcg cagcgacctg
     8221 atcggcagct cgccgcgcca cctggtgccc cgcgaccagc cggtggtgct gggtgaaggc
     8281 ggcggcgaaa gacggcgcac cggcaaggcc gccaatcccg tgggggcgcg gcgcgccccg
     8341 ccgccgctgc ggcgcgggac cgccccgtcg cccgtatccg gcaaaccgcg cggccggcga
     8401 tcctgacggg ccggcccggc gccgcaccgg gcgcggtgga atccgggacg gagatcatgt
     8461 gggggcgtca gcgcgcatgc cgtgccggtg gcccgccgct ggatgaactg acggtgtccc
     8521 ttttcaacgg ggaggtgttg atggatcgga tcctgtgcat caccggtggc agccgcggca
     8581 tcggggccgc cacagcccgc ctggcggccc gccagggcta tgcggtggcg gtcggctacc
     8641 tggggcgacg ggacgccgcc gaggcgctgg tcgctcagat cgtggccaac ggcggtgtgg
     8701 cgcgggccct gcaggtggat gtggcgctgg aagcctccgt ggaggctttc ttcgcggccg
     8761 tggaccgggc cctggggccg ctcacggcgc tggtgaacaa tgccggcgtg ctcgacaccc
     8821 agtgccgcct ggaagacatg gacgcggcgc gcctgcagcg ggtcttcgct accaatgtga
     8881 ccggcagctt cctgtgcgcc cgcgaggcgg tgcggcgcat gagcacgcgc cacggcggcc
     8941 atggcggggc catcgtcaat ctgtcgtcgc gggcggcgcg cctgggttcg cccggcgaat
     9001 acatcgacta cgccgcctcc aagggcgcca tcgacacgct caccgtcggg ctggcaaagg
     9061 aggtggcggc cgagggcatc cgcgtcaatg cggtgagtcc cggcctgatc gccaccgaca
     9121 tccatgcggc tggcggtgag ccgggccgcg tcgcgcgcct ggcctcggcc atcccgatgc
     9181 agcggggcgg cgagccggac gaggtggcgc gggccatcct gtggctgctg tcggacgacg
     9241 cgtcctacgt gacgggggcg aacctggacg tggcgggcgg gcgctgacgc gcgcgcatca
     9301 gccgaggcga cggcgcgagg actgcggcga gggcgcgcat ggcagccgta cgcggcagtc
     9361 cgctgtccac gaagcgccga tcattgaacc gcgcaccgcc acccggtcgt ccgggcgcgg
     9421 tgcgcggcgc cgtcagacgc gcagggcggc gcgcagcttg tccagcagct tctcacccat
     9481 gcctttgacg gccagcaggt cgtcaagacg ggcgaagggg cgggccgcga cgatggcggc
     9541 cgcgaccgtc ttgggcaggc ccttgacggc agagagctca gtggcgctgg cggtgttgat
     9601 gcagacaccg gacgcggggg cggccgattt cgcggaggcc ttgcgggcgg ccggcttctt
     9661 ggcggcaggt gcgggcgcgg cttcggcctc ggcggcctcg atggcctggg cggcgggtgc
     9721 ctcggccgca gcggccgggc tttccggctc ggcggcgaag ctcaccggca gggtgaagtt
     9781 gctgaagtcg cgggtggcgt aggcttcgcc cgtccattcc cgcagcagca gggcgatgtg
     9841 ccaggtgccg gccgggcggg cggcgagggg cagatcgaag tccagggtgt ccacgctctg
     9901 gccaccgccg agcacgccca gggccgccgt ggcgaggcgc gtacccgccg gttcgtcaga
     9961 ggtgtacgga gcggacaggg cccacagttc gaggctcagg ctgccgctca ggttgtcggc
    10021 gtcgcgggga ttgtcgacgc ggtcgacgtg cacggtgacg tggttcgact cgagaccgaa
    10081 accggtgctg ccgacgaggc ggggctgcac gaaacgttcg gtgcgcggga agcgggcggt
    10141 gtcgcagagg gtgtcgaagc ggccgtcgtg accggacacg agggccagca ccatgacgtg
    10201 ttcgccctgg ccggcgggcg gcagtgcggc gacggtggca tcgatccacg cggtgtcgaa
    10261 ggtcgtgtcg ggctggcagc gcagctcggc caccttgata ccggcgggca gcgcgccggg
    10321 cgcgacgctg tcgcaggccc acagttgcag ggcccattcg ccggcggcgg tggcggccgg
    10381 gttggtgatc tgcagttcgg cgttgaggtg gacgacgtcg ccgtcgaagc ggtagccgtg
    10441 ggtctgaccg agacggagcg gggtgtcggg ggtggcgtgc atgatgggag tccagccggg
    10501 atgagggcgc ggattttcaa cccgtcacgg tccccgggtc aacgccgttc cgatgaattt
    10561 tttttgatgc accgattcgg ggcgaaatcc tcgaaagcct tgtggcgact ggcgtgcacc
    10621 gttctggtgc tgttattcca atgggtttgt cggaaagtcg acctagagca gcacgatatc
    10681 gaattgctcc tgcccgtagc tggcctccgg gtgcagcgag acggtcttgt cgatgaagtc
    10741 ggagagcatg ccgagggcct gggactcctc gtcgaggaag aggtcgatga cctgcggcgc
    10801 ggcgagcact cggaactccc gcgcattgaa ctggcgcgcc tcccgcagca gttcgcgcag
    10861 gatctcgtag gccacggtgc gcgcggtctt cacctcgccg cgaccgccgc aggtggggca
    10921 ggtctcgcag agcaggtggg cgagggactc gcgggtgcgc ttgcgggtca tttccaccag
    10981 gcccagactg gtgaagccgt tgaccgtcat cttggtgtgg tcgcgggcca gggccttgcg
    11041 gaactcgtcg agcacggcgg tgcggtgctc ggcgctctcc atgtcgatga agtccacgat
    11101 gatgatgcca cccaggttgc gcaggcgcag ctggcgggcg atggtctggg ccgcctccag
    11161 gttggtcttg aagatcgtgt cgtcgaagtt gcgtgcgccg acgaagccgc cggtgttcac
    11221 gtcgatggtg gtcatcgcct cggtctggtc gatgatcagg tagccgccgg acttgaggtc
    11281 cacgcggcgc gccagggcct tctcgatctc ggcttccacg ccgtgcaggt cgaagagggg
    11341 ccgctcgccg gtgtagtgtt ccaggcgctc ggcgacctga ggcatgtatt cggtggtgaa
    11401 ggccgacagt ttctggaagt tctcgcggga atccgccacg acgcgggtgg tggcgtcggt
    11461 gaccaggtcc cgcagcaccc gctgggcgag gttgagatcc tggtacagca cgtatggcgg
    11521 ccgggcgccg atggtgcgat tgcggatctc ggcccacagc ttgcgcaggt aggcgatgtc
    11581 cgcggcgagt tcgtcgtcgg tagcgttctc ggccatcgtg cgcacgatgt agccgccgct
    11641 ctcatccttg ggcagcaggc ggctgacgcg ttcgcgcagg gcttcgcggc cggcttcgtc
    11701 gccgatgcgc tgggagatgc cgatgtgctt gtcctggggc aggtagacca gcatgcgccc
    11761 ggcgatggag atctgcgtgg acagacgcgc gcccttggtg ccgatggggt ccttgaggac
    11821 ctgcaccatg aggttctgtc cttcggcgag gatgcgctcg atgggacgcg gtgcctcgcc
    11881 cccgtggcgg tcggtccaga tgtcggccac atgcaggaag gcggtgcgtt ccaggcctac
    11941 gtccatgaag gcggactgca tgcccggcag cacgcgcacc acacggccca ggtagatgtt
    12001 gcccacgatg ccgcggcttg cggtgcgctc cacgtgca
//
LOCUS       DAOQMM010000111        11805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2460_length_11805_cov_41.851830, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000111 DAOQMM010000000
VERSION     DAOQMM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2460_length_11805_cov_41.851830"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1099)
                     /locus_tag="PLH85_14655"
     CDS             complement(146..1099)
                     /locus_tag="PLH85_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65856.1"
                     /translation="MTSPIRPLRVPPTPCAPRVMRASDHPLGIDRAIAATLSAVLRPA
                     WQAWLQAPRVRLLERLGPLGRGVIAIAPERSAEDGCSTDLDPALTGRWLTLRVLDERG
                     RVCELSAPDATLAQLADDLDRLDDTRLAHALADCLRQDVLQAVCSDEESPWPVLRHAI
                     PHANAAAELLFLAPEGRFDPGVRGALLPALRQRERQLQEAAHQHLAQGLDRSLAARLH
                     SVGVAPTLTHINWLAGTASDVQARRLHALLIAPRFCRAFLTGDVAARGAFEAAVDAGA
                     SLWSFLEAHYRLPVVGLRDADPPDRSGSHAPRNGPPLRRQG"
     gene            1334..2536
                     /gene="csm6"
                     /locus_tag="PLH85_14660"
     CDS             1334..2536
                     /gene="csm6"
                     /locus_tag="PLH85_14660"
                     /inference="COORDINATES: protein motif:HMM:NF021162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HNB65857.1"
                     /translation="MTNDWSGRAGEGAPEGDGEWALSMPVRDRPPESFARRVLVAVGG
                     LAPQVITEALYWLACVRVPPFVPTEIRVVTTTVGREQCELALLHPQQGKFHALLRDYG
                     LVGQVAFGSEHFELIRDGLGRALDDVCDGPENVAAADSITAFIRDLAADPDCALHVCI
                     SGGRNTMGVYLGYALSLFGRRQDRLSHVMVPPEFTHNHDFYYPPPQPGVLIDRISNRS
                     VSTRAASVTLAEIPFVSLRGLLPSEVLDGVVSYSTAVALTRESMQPHRLVVARASRRL
                     LCGEREVALPPQLFAWYLWLAQRRKSGAEHGGAVRWTDADIAGTFLACYRSVVGIASH
                     DYDTAAEVLRDGMTDTFFAEKKSRVNRALRTALGVDAEAYLIHETGRRPATRFGLLLA
                     PQAIEFRD"
     gene            complement(2627..4543)
                     /gene="thiC"
                     /locus_tag="PLH85_14665"
     CDS             complement(2627..4543)
                     /gene="thiC"
                     /locus_tag="PLH85_14665"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNB65858.1"
                     /translation="MNATDKFIASSAHVDEAAIAPLPNSRKIYVEGSRADLRVPMREI
                     RQADTPTAMGGEQNPPIFVYDCSGPYTDPAARIDIRSGLPALRQAWIAERGDTEVLPD
                     LSSEYGRVRAADSRLDELRFPGLHRAPRRARAGANVTQMHYARRGVVTPEMEFVAIRE
                     NLRRTEYVESLRASGPLGERMAKLLTRQHPGQSYGAAIPATITPEFVRDEIARGRAIL
                     PANVNHPETEPMIIGRNFLVKINANIGNSALGSSINEEVDKMTWAIRWGGDTVMDLST
                     GKNIHETREWIIRNSPVPIGTVPIYQALEKVEGKAEELTWEIFRDTLIEQAEQGVDYF
                     TIHAGVLLRYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEEICEIMKAYDV
                     AFSLGDGLRPGSIYDANDEAQLGELKTLGELTDIAWRHDVQVMIEGPGHVPMHMIKEN
                     MDLQLEYCKEAPFYTLGPLTTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGL
                     PDKNDVKEGIITYKLAAHAADLAKGHPGAQIRDNALSKARYEFRWDDQFNLGLDPDKA
                     KSFHDETLPKESAKVAHFCSMCGPHFCSMKITQDVRDFAAQQGIGEAEALEKGMETKA
                     VEFVRTGAEVYRKV"
     regulatory      complement(4581..4680)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4846..6321
                     /locus_tag="PLH85_14670"
     CDS             4846..6321
                     /locus_tag="PLH85_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNB65859.1"
                     /translation="MRLRMRPLVVLLALAGASQALAADWQTVAADKKRKIELDRASIL
                     QSDPGTKVAWGRIVLADAEAEAAGYATVKALNRYDCRNQTFATIKRVYLDGDAAVLKD
                     EKVKVEKDMPITAGSVDEKLWKEVCQPGGTKGLNQVAQEASKAAQAAAAKPDLRAADY
                     RPAKGAEKPAVRQVNEGKGEEPPATPIKKNFIDKPAPPPKAEVAEKPAEKTAEKAPAP
                     RVEAAPPEVRRVPAYVPPRPRVVRMPAVAVEAGAGHGPDAAGTPLAHHEIHWSYDGDG
                     GPPNWGVLKPEWGLCSTGERQSPIDIREGIKVELEPIRFDYKPSYFRVVDNGHTIQVN
                     LGPGNTLSVMGRQYDLVQFHFHRPSEERINGKGFDMVAHLVHKDLDGRLAVVAVLLER
                     GSAHPLIQTLWNNLPLEKNVEYGPEGVSIDLNQLLPDNRSYYTYMGSLTTPPCSEGVL
                     WMVLKQPAQMSADQIGIFSRLYPMNARPVQSIKGRLIKESR"
     gene            complement(6305..7789)
                     /locus_tag="PLH85_14675"
     CDS             complement(6305..7789)
                     /locus_tag="PLH85_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB65860.1"
                     /translation="MLRITLSPDSPTPLTDQIVAALCARVDDRVLRPGMRLPPIRQFS
                     ADHGVSRFTVVEAYDRLVALGYVQSRRGSGFYVAGRPAPARTGETDTGVERAIDAAWL
                     MREMASGGPERLLAGTGNMPVAWLDEAGVRRALRSLARRPDVRPASYGTAQGYAPLRA
                     QLQVQLAGHGIRATPEQIVLTYGAHQALDILCKYFLKPGDCVLVDDPGYWNLFGNLRL
                     YGVALLGVPRTPEGPDTAALEALLAHHKPRLFFTQSVFHNPTSGCLSPACAFRVLQLA
                     ARHDFMIVEDDIVGDIAGPAATRLASLDQLERVIFVGSFSKTLSGNLRVGFLACRPDL
                     ARLLTDVKIVSMMSSAEFSEQLVHQMLTDGHYRKLLERLRDRIADATGRSLRQLERLG
                     LHPDPEPAGGTFLWVPLPGVEDTAELARVALQDDILLAPGSVFRPQNQPSPYLRVHVA
                     HGDDPRLLRFLARALDDAHKKRASPEGRPGEALTNAADRLSGIP"
     gene            7916..8389
                     /locus_tag="PLH85_14680"
     CDS             7916..8389
                     /locus_tag="PLH85_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65861.1"
                     /translation="MGRGASIRGLIALIGLLPLLALAGGARAQVWWPERQWVFVADRD
                     GGSVQVLALRSGLTPVAVLRQRDRSAVRGIAVDPRAVRAWVLGPRELDLHDAANGRLI
                     AHYAAPAGMLLEGFEPADGRSLRVRGGGALFEPRPGCGCLARVDAVLTGREMRAP"
     gene            8490..10544
                     /locus_tag="PLH85_14685"
     CDS             8490..10544
                     /locus_tag="PLH85_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB65862.1"
                     /translation="MTQHGSAPWPLGRRRTFAAFVLTGGVLASRAWSGSDVDALAELP
                     LEDLLQVEVSTVSRHAQRLAEAAAAVHVITAEEIRHAGVRTLPDALRLAPGVDVAQIG
                     ADRWAVSLRGHTDYLANKLMVLVDGRSVYSPNFSGVLWNTVQVPLENIERIEVIGGPG
                     GALWGANAVNGIINIITRPAQATQGTLVNAGHGSAEGSFGLARQGGRFDEDTFYSAYV
                     SATRGAGLAAAGREDRPDGNVDSGAGLRIDARRGRDAWMLNSAVYRSRVGGTGRVPDP
                     LAAGAGFGRNVALADDFSGVDVHGRLSRQLDDGGEVQASVAWSHADLDSGLLARERQD
                     IVDMEVQHRLPPRSGHRLTWGAGLRGFMDDARDGAVTHLTRTRETLVVASLFAQDEIA
                     VTGTVRLTLGARLDQQKYTGAGLQPSARLLWQPAPGQTAWAAVSQALRLPSRGERTTD
                     YRLGVLPPASPLNPAPLPALLEVRGSGGYGAEHLTAWEAGWRGQPGAGLVLDASVFLH
                     RYRGLRTASGGPPEMAASASYMVIPALFSNLGEQTLRGVEAAAHWRPVEGWRLRAAAT
                     YRVTSPFEAGATMFQDLFASQAPRRAANLLSGWRLGSRTDLDLWLVRVGPRNVPGQAA
                     LPASNRMDLRLARRLDRRVEVALVGQNLLDDRHGEYVSFVPGFAPVEVRRGIYGQIRV
                     SD"
     gene            10719..>11805
                     /locus_tag="PLH85_14690"
     CDS             10719..>11805
                     /locus_tag="PLH85_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNB65863.1"
                     /translation="MNFYNEMHDGAGGVRAHYERYAAWLASSPAERIQRKRAEADLAF
                     RKVGITFAVYGDASDGDGGKERLIPFDIIPRVIPAPEWRDLSAGLRQRVQTLNAFLHD
                     IYHDQEILKAGVIPAEQVLNNAQFRPEMMGVDVVGNIYAHIAGVDVVRAGKGEFYVLE
                     DNLRVPSGVSYMLEDRKMMMRLFPELFSIHKVAPVQHYPDLLLEKLRAVAPKGISDPT
                     VVVLTPGAYNSAYFEHTFLAQQMGVELVEGRDLFVEDDTVYMRTTQGRRRVDVIYRRV
                     DDDFLDPTVFRPDSMLGVRGLLAAYKAGNVTLANAIGTGVADDKSIYPYVPDMVRFYL
                     GEEPKLNNVPTYQCRKPEDLAYTLAHLP"
BASE COUNT         1819 a         3722 c         4319 g         1945 t
ORIGIN      
        1 gtgggcatgc agggcatgtc cgatggtatc cacgatgaac gagtcggtga ccatgagggt
       61 tccccggaac ggaagggcaa tgcatcgttg gcgtgcatga tacgcctcct ggcgagggcc
      121 ggcgcggcat gccggccgga cgaggtcagc cctgccggcg gagcggcggg ccgttgcggg
      181 gcgcgtgact gcccgagcgg tccggagggt cggcgtcccg caggcccacg accggcaggc
      241 ggtaatgggc ttctaggaag gaccacagcg acgcgcccgc atcgaccgcc gcctcgaagg
      301 ctccccgcgc cgccacatcg ccggtcagga atgcccggca gaagcggggg gcgatgagca
      361 gtgcgtggag gcgacgcgcc tgcacgtccg acgccgttcc tgccagccag ttgatgtggg
      421 tgagggtggg cgccacgccg acggagtgca ggcgggccgc cagggatctg tccaggccct
      481 gcgccaggtg ctggtgggcc gcctcctgca gttgccgctc gcgctggcgc agggcgggca
      541 gcagcgctcc ccgcacgccc gggtcgaagc gtccttccgg cgcgagaaag agcaactccg
      601 cagcggcgtt ggcgtgggga atggcgtggc ggaggacggg ccacggggat tcttcgtcgc
      661 tgcagaccgc ctgcaggaca tcttggcgca agcagtcggc gagtgcgtga gcgaggcgcg
      721 tgtcgtcgag gcggtcgaga tcgtcggcga gctgcgccag cgtcgcgtcg ggggcgctca
      781 gttcgcacac gcgaccacgc tcgtcgagca cccgcagggt cagccaccgg cccgtcagtg
      841 cggggtcgag atcggtgctg cagccgtctt ccgcgctgcg ctccggggcg atggcgatca
      901 cgccgcgtcc gagcgggccg aggcgttcga gcaggcggac gcggggggcc tgcagccatg
      961 cctgccaggc cggacgcagg actgccgaaa gggtcgccgc gatggcgcgg tcgatgccga
     1021 gcgggtggtc gctggcgcgc atgacgcgcg gggcgcaggg ggtgggcggg acgcggaggg
     1081 ggcggatcgg tgacgtcatc gtagtgagct cccgtgaaat gcggtctggt gccgcgatga
     1141 gcccagctta atgcgcgccc cccgcgatgc gccgacatga caacgttgcc gccagggtca
     1201 tcgcttcagg ggcgcgggag cccgctgcgc cgaccgattc ccgcgccggc tgcaggcttt
     1261 ttcaagacaa gcttgccatg gtgaatatct gtcacgcgta tgcgctacag tgcgaggctt
     1321 ggggagaaaa gcgatgacga acgactggag tggccgggcg ggcgaaggtg caccggaagg
     1381 cgacggggag tgggccttgt cgatgccggt cagggaccgg ccgcccgagt cctttgcccg
     1441 ccgtgtgctg gtggcagtgg gcgggctggc gccgcaggtg atcacggagg ccctgtactg
     1501 gctggcctgt gtgcgggtgc cgcctttcgt acccaccgag atccgcgtcg tcaccaccac
     1561 cgtcgggcgc gagcaatgcg agctggcgct gcttcatccc cagcagggca aattccacgc
     1621 cctactgcgg gattacggcc tggtcggcca ggtggccttc ggcagtgaac acttcgagct
     1681 gatccgcgac ggcctcgggc gggcgctgga cgacgtctgc gatgggcccg agaacgtggc
     1741 cgcggcggac tccatcaccg ccttcatccg cgatctggcg gccgatccgg attgcgcgct
     1801 gcacgtgtgc atctcgggcg ggcgcaacac catgggcgtc tatctcggct atgccctgag
     1861 cctgttcgga cggcgccagg atcgtctgtc ccacgtgatg gtgccgccgg aattcaccca
     1921 caaccacgat ttttactacc cgccgcccca gcccggggtg ctcatcgacc gcatcagcaa
     1981 ccgttccgtc agcacccgtg cggcgtcggt caccttggcg gagatcccct tcgtcagcct
     2041 gcgcggcctg ctgccgagcg aggtgctgga cggcgtggtg agctattcca ccgcggtcgc
     2101 cctgacccgt gaatccatgc agccccaccg gctggtggtg gcccgggcga gccggcggct
     2161 gttgtgcggc gagcgggagg tggccctgcc gccccagctg ttcgcctggt atctgtggct
     2221 ggcccagcgc cgcaagagcg gggcggaaca tggcggtgca gtgcgctgga ctgatgcgga
     2281 catcgccggc accttcctgg cctgctaccg gagcgtggtg gggatcgcct cccacgacta
     2341 cgacacggcg gcggaggttc tgcgggacgg catgaccgac acctttttcg ccgagaagaa
     2401 gtctcgcgtc aatcgggctc tgcgtacggc cctgggcgtg gatgcggagg cttacctgat
     2461 ccacgagact ggccgccgtc cggccacccg cttcggcctg ctgctggcgc cgcaggcgat
     2521 tgagttccgc gactgaggcc tgcgtgccgc ggagccgagg cgtcctgaaa agtcggacgg
     2581 ggcccgcggg ccccgtccat cgggtgatgc cgatgcgcgg acgcgctcac accttccgat
     2641 acacctcggc gccggtgcgc acgaactcga cggccttcgt ttccatgccc ttctccagcg
     2701 cctcggcctc gccgatgccc tgctgggcgg cgaagtcgcg cacgtcctgg gtgatcttca
     2761 tggagcagaa gtggggcccg cacatggagc agaagtgggc gaccttggca gactccttgg
     2821 gcagggtctc gtcatggaag ctcttggcct tgtccggatc gaggcccaga ttgaactggt
     2881 cgtcccagcg gaactcgtag cgggccttgg acagcgcgtt gtcgcggatc tgggcgccgg
     2941 gatggccctt ggccagatcg gcggcgtggg ccgccagctt gtacgtgatg atgccttcct
     3001 tcacatcgtt cttgtccggc aggcccagat gctccttggg cgtcacgtag cacagcatcg
     3061 ccgtaccgta ccagccgatc tgagccgcac cgatgccact ggtgatgtga tcgtaacccg
     3121 gcgcgatgtc ggtggtgagc ggacccagcg tgtagaacgg cgcctccttg cagtactcca
     3181 gctgcagatc catgttctcc ttgatcatgt gcatgggcac atgacccggc ccctcgatca
     3241 tcacctgcac atcgtgccgc caggcaatgt cggtgagctc gccgagcgtc ttcagctcgc
     3301 ccagctgggc ttcatcgttg gcatcgtaga tcgaaccggg gcgcagaccg tcgcccaggg
     3361 agaaggccac atcgtaggcc ttcatgatct cgcagatctc ctcgaagtgc gtgtagagga
     3421 agctctcctt gtgatgggcc aggcaccact tggccatgat cgaaccgccg cggctgacga
     3481 tgccggtcat gcggttggcc gtgaggggca cataacgcag cagcaccccg gcgtggatgg
     3541 tgaagtaatc cacgccctgc tcggcctgct cgatcaacgt atcgcggaag atctcccagg
     3601 tgagctcctc ggccttgccc tccaccttct cgagggcctg gtagatgggc accgtgccga
     3661 tcggcaccgg gctgttgcgg atgatccact cgcgggtctc gtggatgttc ttgccggtgg
     3721 acagatccat caccgtgtcg ccgccccagc ggatcgccca ggtcatcttg tccacttctt
     3781 cgttgatgga ggagcccagg gccgagttac cgatgttggc gttgatcttc accaggaaat
     3841 tgcggccgat gatcatcggc tcggtctccg gatggttgac gttggccggc aggatggcgc
     3901 ggccgcgggc gatctcgtcg cgcacgaact ccggcgtgat cgtcgcgggg atggcggcgc
     3961 cgtagctctg gccggggtgt tggcgggtca ggagtttggc catgcgttcg cccagggggc
     4021 cgctggcgcg cagggactcc acgtattcgg tgcggcgcag gttctcgcgg atcgccacga
     4081 actccatctc gggcgtgacg acgccgcggc gggcgtagtg catctgggtc acgttggcgc
     4141 cggcgcgggc gcgccgcggt gcgcggtgca gaccggggaa acgcagctca tcgaggcgtg
     4201 aatcggcggc gcggacgcgg ccgtattcgg aggacaggtc gggcagcacc tcggtgtcgc
     4261 cgcgctccgc gatccaggcc tggcgcaggg caggcaggcc ggagcggatg tcgatgcgcg
     4321 cggccgggtc ggtgtagggg ccggagcagt catagacgaa gatcggcgga ttctgttcgc
     4381 cgcccatggc ggtgggcgtg tcggcctggc ggatctcgcg catgggcacg cgcagatcgg
     4441 cgcgggagcc ttccacgtag atcttgcggg agttgggcag gggcgcgatg gccgcttcgt
     4501 ccacgtgggc ggacgaagca atgaatttgt cggtggcgtt catgaaggct cccaggggag
     4561 ggccgcgggc gggggaaacg acggcgggag ccacgtttcc ctacgccggt acgacccgga
     4621 tcaggttcgg agggtaagtc tcagccgcgc gcatgggcag cggcacccct aacgtatggc
     4681 ccggaaagta ctggaagcgg ccggtcattg caagattttc cggaaaatca aggctccgcg
     4741 ccattccccg gcgctgcgga gcgtttctaa agctttgcgg caccccgccg aaataggagg
     4801 catccggtcc attgcggccc ctgcatccct tttcggagaa tcaccatgcg actgcgcatg
     4861 cgtcccctcg tcgtcctgct ggccctggcc ggcgcgtccc aggccctggc cgccgactgg
     4921 cagaccgtgg ctgcggacaa gaagcgcaag atcgaactcg accgggcgag catcctgcaa
     4981 tccgatccgg gcaccaaggt ggcctggggg cgcatcgtgc tggccgatgc cgaggccgag
     5041 gcggctggct acgccaccgt gaaggccctc aaccgctacg actgccgcaa ccagaccttc
     5101 gccaccatca aacgcgtcta tctggatggc gatgcagccg tgctgaagga cgagaaggtc
     5161 aaggtcgaga aggacatgcc catcaccgcg ggcagcgtgg acgagaagtt gtggaaggag
     5221 gtctgccagc ccggcggcac caaggggctc aatcaggttg cccaggaggc gtcgaaggcg
     5281 gcccaggccg cggccgccaa gccggatctg cgtgcagccg actatcgtcc cgccaaaggt
     5341 gccgaaaagc ccgcggtgcg tcaggtgaat gaagggaagg gtgaagagcc gccggcgacg
     5401 ccgatcaaga agaatttcat cgacaagccg gcaccaccgc ccaaggcgga ggtggccgaa
     5461 aaacctgccg agaagaccgc cgagaaggcg ccggcacccc gtgtcgaagc ggctccgccg
     5521 gaagtgcgtc gcgtgccggc ctatgtgccg ccgcggccgc gggtggtgcg catgcccgcc
     5581 gtggcggtgg aggcgggcgc cggccatggc ccggatgccg ccggcacgcc gctcgcccat
     5641 cacgagatcc actggagtta cgacggcgac ggcggcccgc ccaactgggg cgtgctcaag
     5701 cccgaatggg ggctgtgcag caccggcgag cgccagtcgc cgatcgacat ccgtgagggc
     5761 atcaaggtgg agctggagcc gatccgcttc gactacaaac cctcatattt ccgcgtggtg
     5821 gacaacggcc atacgatcca ggtcaacctg ggtccgggca ataccctctc ggtgatgggg
     5881 cgccagtacg atctggtgca gttccacttt caccggccgt cggaggagcg catcaacggc
     5941 aagggcttcg acatggttgc gcacctggtc cacaaggacc tggacggccg tctggccgtg
     6001 gtggccgtgc tgctggagcg gggcagcgcc catccgctga tccagaccct gtggaacaac
     6061 ctgccgctgg agaagaatgt ggagtacggc ccggaggggg tgtccatcga tctcaaccag
     6121 ctcctgccgg acaaccgcag ctattacacc tacatgggtt ccctcaccac gccgccctgc
     6181 agcgagggcg tgctgtggat ggttctgaag cagccggcgc agatgtctgc ggaccagatc
     6241 ggcatcttct cgcgcctgta tccaatgaac gcccgcccgg tgcagtcgat caaggggcgt
     6301 ctcatcaagg aatcccgctg agccggtccg ccgcattcgt gagtgcctct ccggggcgtc
     6361 cttcagggga cgcccttttt ttgtgtgcgt cgtccagggc gcgggccaga aagcgcagca
     6421 ggcgtgggtc gtcgccgtgg gccacgtgca cgcgcagata gggggagggc tggttctggg
     6481 gacggaacac gctgcccggc gcgaggagga tgtcatcctg aagcgcgacg cgggcgagct
     6541 cggcggtgtc ctccacgccc ggcaggggca cccacaggaa ggtgccgccg gcaggttcgg
     6601 ggtcgggatg caggccgagg cgctccagct gccgcaggct gcgtccggtg gcatcggcaa
     6661 tccggtcgcg cagacgctcc agcagcttgc ggtagtggcc gtcggtgagc atctggtgga
     6721 cgagctgctc ggagaactcc gccgaggaca tcatcgaaac gatcttcacg tcggtgagga
     6781 ggcgtgccag atcgggccgg caggccagga agccgacccg caggttgcct gacagggtct
     6841 tggagaagct gcccacgaag atcacccgct cgagctggtc gaggctggcc aggcgggtgg
     6901 cagcggggcc ggcaatgtcg ccgacgatgt cgtcctcgac gatcatgaag tcgtggcggg
     6961 ccgccagttg cagtacgcgg aaggcacagg ccgggctgag gcagcccgac gtcgggttgt
     7021 ggaacaccga ctgggtgaag aacaggcgcg gcttgtggtg ggcgaggagg gcttccagcg
     7081 ccgccgtatc cgggccctcc ggcgtgcgcg gcacgcccag cagggcgaca ccgtagaggc
     7141 gcaggttgcc gaacagattc cagtagccgg gatcgtccac cagcacgcag tcgccgggtt
     7201 tgaggaagta tttgcagagg atgtccaggg cctgatgggc gccgtaggtg aggacgatct
     7261 gttccggtgt ggcgcggatc ccgtggccgg caagctggac ctgcaactgg gcgcgcaggg
     7321 gcgcgtagcc ctgggcggtt ccgtagctgg ccgggcgcac gtcagggcgc cgcgcaagac
     7381 tgcgcagggc gcgtcgtacg cccgcctcgt cgagccacgc cacgggcatg ttgccggtgc
     7441 cggcgagcag gcgctccgga ccgccggagg ccatctcgcg catgagccac gctgcatcga
     7501 tggcgcgctc cacgccggtg tccgtctcgc cggtgcgggc gggtgccggc cggccggcca
     7561 catagaagcc ggagccgcga cgggactgca cgtaacccag ggccaccagg cggtcatagg
     7621 cttccaccac cgtgaagcgg gagacgccgt ggtccgcgga aaactggcgg atgggcggca
     7681 ggcgcatgcc aggccgcagg acgcgatcat ccacgcgggc gcagagggcc gcgacgatct
     7741 ggtcggtgag gggggtcggg ctgtctggcg acagggtgat gcgcagcatg gctttgacct
     7801 cggcgtaccg gtcgaagggc cggtacggtg tagtgagttg tccgaaaatt gtatctgtga
     7861 tgttgaaaaa gtgtccgcta ttgtcaggct cattgagctt gaggggagtg cgggcatggg
     7921 acggggagca agcatccgtg ggctgatcgc cctgatcggt ctgttgccgc tgctggcgct
     7981 ggcgggcggg gcgcgtgcgc aggtgtggtg gccggagcgg caatgggtct tcgtggcgga
     8041 tcgggacggc ggatcggtcc aggtgctggc gctgcgctcc ggcctgacgc ccgtggccgt
     8101 gctgcgccag cgcgaccgga gcgctgtgcg tggcatcgcg gtcgatccgc gggccgtgcg
     8161 ggcctgggtg ctggggccac gggaactgga tctgcatgac gcggccaacg ggcggctgat
     8221 cgcccactat gcggcaccgg caggcatgct gctggaaggc ttcgagccgg ccgacggacg
     8281 ttccctgcgg gtgcgtggtg gcggggccct gttcgaaccg cggccaggtt gcggatgtct
     8341 ggcgcgggta gacgccgtgc tcaccggtcg ggagatgcgc gcgccctgat gcatctcaag
     8401 tccgtggatg accggtacgt taagatctgt ggtgcgtgaa cggatcaatg gctgggggac
     8461 ggaggcggga tgcggatcgg gggcggtagg tgacacagca tggcagcgcg ccgtggcccc
     8521 tgggccggcg ccgcaccttt gccgcgttcg tcctgactgg cggggttctg gcctcgcgcg
     8581 cgtggagcgg ttccgacgtc gatgccctgg cggaactgcc ccttgaggat ctcctgcagg
     8641 tggaggtgag cacggtcagc cggcacgccc agcgtcttgc ggaggcggcc gctgcggtgc
     8701 atgtgatcac cgccgaggag atccgccacg cgggggtgcg caccctgccg gatgccttgc
     8761 ggctggcgcc cggcgtggat gtggcgcaga tcggtgcgga ccgctgggcc gtgtcgctgc
     8821 gggggcatac ggactacctc gccaacaagc tgatggtgct ggtggacggg cgcagcgtct
     8881 atagccccaa tttctccggc gtgctgtgga acaccgtgca ggtgcccctc gagaacatcg
     8941 agcgcatcga ggtgatcggc ggcccggggg gcgccctgtg gggggcgaac gcggtcaatg
     9001 ggatcatcaa catcatcacg cgaccagcgc aggccacgca gggcacgctg gtcaatgccg
     9061 ggcatggctc tgcggagggg agtttcggac tggcccggca gggtggccgg ttcgacgagg
     9121 acactttcta cagcgcctac gtgagtgcca cgcgcggcgc cggactggcc gcggcggggc
     9181 gtgaggatcg accggacggc aatgtcgaca gcggggccgg gctgcggatc gatgcccgtc
     9241 gcgggcgcga cgcgtggatg ctcaacagtg ccgtctaccg cagccgggtg gggggcacgg
     9301 ggcgggtgcc ggatccgctg gcggctggag cgggctttgg gcgcaacgtg gctctggcgg
     9361 acgatttttc cggagtggat gtgcacggcc ggctctcccg tcagctggac gacgggggcg
     9421 aggtgcaggc cagcgtggcc tggtctcacg ccgatctgga ctcgggcctg ctggcccgcg
     9481 aacgccagga catcgtggac atggaggtcc agcaccgcct gccgccccgg tcggggcacc
     9541 gcctgacctg gggtgcgggt ctgcgcggct tcatggacga tgcgcgggat ggggcggtga
     9601 cccatctcac gcggacgcgg gagactctgg tggtggcgag cctgtttgcc caggatgaga
     9661 tcgccgtcac ggggaccgtg cgcctgacac tcggcgcgcg tctcgatcag cagaagtaca
     9721 ccggcgccgg gctgcagcct tctgcgcgtc tgctctggca accggcgccc gggcagaccg
     9781 cgtgggccgc cgtctcccag gccctgcgcc tgccctcccg cggagaacgc acgaccgatt
     9841 accggctcgg cgtgctgccg ccggccagtc ccctcaatcc ggcgccgctg ccggcactgc
     9901 tggaggtgcg aggttccggt gggtacgggg cggaacacct gacggcctgg gaggccggct
     9961 ggcgaggtca gccgggggcc ggtctggtgc tcgatgcgtc ggtcttcctg caccgctacc
    10021 gtggcttgcg cacggcgtcc ggcggaccgc ccgaaatggc ggcctccgct tcctatatgg
    10081 tgattcccgc cctgttttcc aatctgggcg agcagaccct gcggggcgtc gaggcggcag
    10141 cccattggcg cccggtggaa ggctggcgtc tgcgcgccgc ggccacctac cgcgtgacgt
    10201 cgcccttcga ggcgggcgcg acgatgtttc aggatctgtt tgccagtcag gcgccgcggc
    10261 gagccgccaa cctgttgtcc ggctggcggc tgggcagccg gaccgacctg gacctgtggc
    10321 tcgttcgcgt cggtccgcgg aacgtgccgg ggcaggcggc gctgccggcc agcaaccgga
    10381 tggacctgcg tctggcccgg cgcctggacc gtcgtgtgga agtggcgctg gtcgggcaga
    10441 atctgctgga tgaccggcat ggggagtatg tctctttcgt gccgggcttt gcgccggtcg
    10501 aagtccggcg gggtatatat ggacagatcc gcgtcagtga ttgatttccg tttctggcac
    10561 cgttttggtg cggccgtctt tccagaccgg tgcgcactgt ccgaagcggg gcacgaggcg
    10621 gcatgtcttg ttattaaggt gttgaatttt attgaaagag taccttggca tggattctgc
    10681 gacagacttt ccattcttag gtacagggag gcccgagcat gaatttctat aacgaaatgc
    10741 acgatggcgc cggcggggta cgtgcccact acgagcgcta tgcggcatgg ctggcgtcct
    10801 cgccggcgga gcgtatccag cgcaagcgcg cagaggccga tctggcgttc cgcaaggtcg
    10861 gcatcacctt cgccgtgtac ggcgatgcgt ccgacggcga tggcggcaag gagcgtctga
    10921 ttcccttcga catcatcccg cgggtgatcc ccgccccgga gtggcgcgac ctgtcagccg
    10981 gcctgcggca gcgggtccag accctgaatg ccttcctgca cgacatctat cacgatcagg
    11041 agatcctcaa agccggcgtg atcccggccg agcaggtgct caacaatgcc cagttccgcc
    11101 cggagatgat gggggtggac gtggtgggca acatctacgc ccacatcgcc ggcgtggacg
    11161 tggtacgggc cggcaagggc gagttctatg tgctggagga caacctgcgg gtgccgtcgg
    11221 gcgtgtccta catgctcgaa gaccgcaaga tgatgatgcg cctcttcccc gagctgttct
    11281 ccatccacaa ggtggcaccg gtgcagcact atcccgacct gctgctggag aagctgcgcg
    11341 cagtggcgcc caagggcatc tccgatccca ccgtggtcgt gctcacgccg ggggcctaca
    11401 acagcgccta cttcgaacac accttcctgg cccagcagat gggcgtcgag ctggtggagg
    11461 ggcgtgacct gttcgtggaa gacgacaccg tctacatgcg taccacccag ggccgccgtc
    11521 gcgtggatgt gatctaccgg cgcgtggacg acgacttcct cgatcccacg gtgttcaggc
    11581 ccgattccat gctgggtgtg cgcggcctgc tggcggccta caaggccggc aacgtgaccc
    11641 tcgccaacgc catcggcacc ggcgtggcag acgacaagtc gatctacccc tatgtgccgg
    11701 acatggtccg cttctacctg ggcgaagagc ccaagctcaa taacgtgccg acctaccagt
    11761 gccgcaagcc ggaggatctg gcctataccc tggcgcacct gcccg
//
LOCUS       DAOQMM010000112        11770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2471_length_11770_cov_41.288690, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000112 DAOQMM010000000
VERSION     DAOQMM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_11770_cov_41.288690"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1268)
                     /locus_tag="PLH85_14695"
     CDS             complement(75..1268)
                     /locus_tag="PLH85_14695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNB65864.1"
                     /translation="MHPSLSLPLLALAFAAPLAQADAPRNYIVQFHDTAVQSPPRALV
                     RDHRVNSDPSGWAYLDARVMERLQSIESRHGIVARHAFSHVLKGFAATLTDAQLARLK
                     SDPAVMSIEEDLPVQIVGQTIPWGISTTRATLSSTLAGNGSGAVNGVNVYVIDTGIAP
                     HGDLNKVNHLNFTADGKNYDCHGHGTHVAGTIGARDDTTSVVGMAPYVRLTGVKVLDC
                     TGSGSDSTVIKGLDWVTANAVKPAVANMSLGGGASSALDAAVTRAVNAGVVFAIAAGN
                     SSAKACNYSPARIGGSLNGAITTAATDSSNKEASFSNYGSCVDTWAPGVSILSTYLNS
                     GVATMSGTSMASPHVAGAAALYLARNPTASPAAVEAAIKQAAVRTGTVSKDGRAITRL
                     DVIGF"
     gene            complement(1439..2131)
                     /locus_tag="PLH85_14700"
     CDS             complement(1439..2131)
                     /locus_tag="PLH85_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNB65865.1"
                     /translation="MSWPTLPAVAEGFAAAGSTLENVRRDFVEWHLGRPRYALWALDV
                     DTACVRRILDAAQGHLADLLLDTYRRQAHVTLALRGFPAATPRRHDEFGAAGLDADLA
                     ALTRRAPTSFLLETGGLASFTSAPFISVSDPDGGIARLRTALHDSDSDPGGPYTPHIT
                     VGLYAGCWPTGAVAERLHAHPLPTQTLHIERVCLMAYAPPEIGGPLETLGSFDLARRC
                     WTWHTATPFITP"
     gene            2410..3057
                     /locus_tag="PLH85_14705"
     CDS             2410..3057
                     /locus_tag="PLH85_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB65866.1"
                     /translation="MELLSTFIDIVLHLDVHLAALTAQYGTWIYAILFAIIFCETGLV
                     IFPFLPGDSLLFVAGALAATGGMDITTLCGVLLAAAVLGDNTNYWIGHTLGPKVFRWE
                     NSRFFNRAAFDKTHAYFEVHGGKTIIVARFLPLLRTFAPFVAGVARMTYARFVALDFI
                     GGCVWILSLTWLGYGFGNLPEVKKNLSLVIVGIIALSLMPVVVGWLRGRSARAAN"
     gene            3067..3711
                     /gene="purN"
                     /locus_tag="PLH85_14710"
     CDS             3067..3711
                     /gene="purN"
                     /locus_tag="PLH85_14710"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNB65867.1"
                     /translation="MKNIVILISGRGSNMEAIVRARIDGARIAAVIANRPDAAGLQFA
                     AHAGIATRVVDHKAFASREAFDAALADVIDGFAPDLVVLAGFMRVLTDGFVQRYTGRL
                     LNIHPSLLPSFPGLHTHQRALEAGVRVHGTTVHFVTPALDCGPIVIQAAVPVRPGDDE
                     DRLAARVLVQEHRIYPQAVRWFVEGRLSLTPAGTVQVADVADGPDGWTVPPVEG"
     gene            3721..4812
                     /locus_tag="PLH85_14715"
     CDS             3721..4812
                     /locus_tag="PLH85_14715"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNB65868.1"
                     /translation="MAVGRIRNRALAIAVALSLLVHGLAILSPAWRLPSDDATVLPPL
                     DARLTPLPPPVLQPSDEKRHLLGEAPPPRPRPRPQARPPALPAGPVAELPADPAGAPE
                     LAGPASNDSAAPPTPDSATEAPPATPVEPNAADVPAGGADLGELWAASGKVQYNVIRG
                     ERNFIIGRTTHAWTHDKEHYSMETVIETVGLVGLIKPFRMVQRSEGRIGAHGLLPDKF
                     TVERDGQVKERADFDWSTAKVVLMAGDRRREFDLAEGDQDVLSLMHQLSLQPDGPMNA
                     ELLVVTGKSAVRSAIRNLGLEDLEVPAGRVSAYHLGSTGHNGELKIDIWLAKDYANLP
                     VRLKITDKNGDVLDQVATGVDLGPRADKK"
     gene            4883..6145
                     /locus_tag="PLH85_14720"
     CDS             4883..6145
                     /locus_tag="PLH85_14720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNB65869.1"
                     /translation="MTITASQIQSASQALRAVLRFDYPADAVLSRYFRDLRELGARDR
                     AFVAEAVYGVLRRKRMLERLCGPEVSPRQLVLAWLARVEGLTLRQLGEGLHGRDTDFF
                     TAIKGRELPEGSLAETTDLPEWLAERLLATRTPEQVQALAQSLNRPAPLDLRVNPLKL
                     DRDALIEKLRADGIACAAAPLSPLAVRLEGKPALQKHPLFLDGSFEVQDEGSQLLGFL
                     VQPKRGEMVVDFCAGAGGKTLLLGALMRNSGRLYAFDVSDKRLAKLKPRVARSGLSNV
                     HPVVIASENDPRVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPESVAELTAKQA
                     AILASAARLVKAGGRLVYATCSLLPEENEAIVAAFLAAHPGFVQVSAGEILGKQGITV
                     GDAEQLHLSPAEHDTDGFFAAVLERSAG"
     gene            6142..6774
                     /locus_tag="PLH85_14725"
     CDS             6142..6774
                     /locus_tag="PLH85_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNB65870.1"
                     /translation="MIGRGAQALLLACAVATADAQTPGVEPTRTVAMHGTAEVAFAPW
                     SDPEALLLQAIDDARDSIHVQAYLFTSKALARGLIAARRRGLRVEVLLDAEMNRPGSL
                     SVLPELLEAGVPVAMLTRFNIAHNKVMLFDADTPHAALATGSYNYTRSARLSNAENLL
                     ILRGNPALVRLYLDTWQRQRAEAVPLRSLDDLPARKDKRDGRESDRTTVD"
     gene            6740..8059
                     /locus_tag="PLH85_14730"
     CDS             6740..8059
                     /locus_tag="PLH85_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNB65871.1"
                     /translation="MEENQIAQLLIDTWADLQEPAIFWQVGVLALCLLLAGLISRQLR
                     QIIARRRSVRDDAAAAGAGEEGLRRVIFPLTALVLVLLARIVLHKWHHVNLLKLAVPL
                     LLAGAAIRLIVHALKRAFPQAGWLASFERIFSTLAWLAVALHITGVLPTVIDGLEQIG
                     FSIGTAHVNLWMLFQGALTVLLTVLVALWVSGSIESRLMATEGLDANLKLVFARLAKA
                     LLLVLAVTIGLPLVGIDLTALSVFGGALGVGLGFGMQKIAANYVSGFILLLDRSIRLG
                     NLISVGTERGVVSQITTRYTVLKGMTGIESIVPNETLVGSVVQNETFTDPRVRIALPV
                     QVSYACDLERALTILVESAKTQPRVLTDPGPGAFVEAFADSGINLQLGFWIADPHEGT
                     LGIRSAINLEIWRRFKAEGIEIPFPQREVRLLNPPAPDVAPPPQSPA"
     gene            8159..9331
                     /locus_tag="PLH85_14735"
     CDS             8159..9331
                     /locus_tag="PLH85_14735"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNB65872.1"
                     /translation="MSSGLIDLPWWGYIVVTLALTHVTIASVTIFLHRHQAHRALELH
                     PLPALFFRTWLWLTTGMVTKEWAAIHRKHHAKCETPEDPHSPQIVGINKVLWTGVFLY
                     VKEARNKDTLQRYGHGTPDDWFERHIFSAAPKLGIVITLIVDSIVFGVWPGVLIWGVQ
                     MVWIPFWAAGVINGLGHFWGYRNYNCADASTNLIPWGILIGGEELHNNHHSFATSAKL
                     SARWYEFDIGWVYIRSLEILGLARVKKVIPTPRFGVAKARADFDMLQAVITHRYDVMT
                     RYVQSLGQIAKDEFEALKAQSGGKLNIKDVRRWLTADEAQLGADERQRLATLLKDNAR
                     LQTVVAMRQELTALWARSTATREQLLQQLQDWIARAEGSGIRQLEDFSLRLRRYAA"
     gene            9484..10038
                     /locus_tag="PLH85_14740"
     CDS             9484..10038
                     /locus_tag="PLH85_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HNB65873.1"
                     /translation="MLLNKPYGVLCQFTDEAGRATLADYVPVPGVYAAGRLDADSEGL
                     LLLTDDGALQARIADPRHKLPKTYLVQVEGVPDVTALESLRRGVDLGDFVTRPCSARL
                     VPEPDWLWPRDPPIRERKAIPVSWLEIVLREGKNRQVRRMTAKVGHPTLRLIRVAIGP
                     WTLGNLTPGAWRDDTIREKSVKNT"
     gene            10122..10391
                     /locus_tag="PLH85_14745"
     CDS             10122..10391
                     /locus_tag="PLH85_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65874.1"
                     /translation="MKKLLSALVAGLFAATTAVSAADLPATPAAAPAAVSAPATPAPT
                     VKKAKKAKKSKKSSAAKAEKKAKKDKKAGKDKKHKKHKKHKKAAV"
     repeat_region   10508..11769
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10580..10614
                     /rpt_unit_seq="gcttcaatgaggccggggcgggagccccggaacat"
BASE COUNT         1903 a         4140 c         3860 g         1867 t
ORIGIN      
        1 cggggcgtga gggggaggcg tgcggcgtcc cgccatcgat caacgatgac gggagcgggg
       61 tggtcgcggt ggcgtcagaa gccgatcaca tcgagccggg tgatggcgcg gccgtccttg
      121 ctgacggtgc cggtgcgcac ggctgcctgc ttgatggccg cttccacggc ggccgggctg
      181 gccgtcgggt tgcgcgccag gtagagcgct gcggcgcctg ccacatgggg gctggccatg
      241 gacgtgcccg acatggtggc cacgccggaa ttgaggtagg tggacaggat cgaaacccct
      301 ggcgcccagg tgtccacaca actaccgtag ttgctgaacg aggcctcctt gttgctgctg
      361 tcggtggcag ccgtggtgat cgcaccgttc agcgagccgc cgatgcgtgc tggcgagtag
      421 ttgcaggcct tggcgctgct gttgccggcg gcaattgcaa agacgacgcc cgcattgacc
      481 gcgcgggtca cggccgcatc gagggccgaa ctggcgccgc cgcccaggct catgttggcc
      541 actgcgggct tgaccgcgtt ggcggtgacc cagtcgagtc ccttgatcac ggtcgaatcc
      601 gagcctgaac ccgtacagtc gagcaccttg acccccgtca ggcggacata gggcgccatg
      661 ccgaccaccg aagtcgtgtc gtcccgggcg ccgatggtgc ccgccacgtg ggtgccgtgg
      721 ccatggcagt cgtagttttt gccgtcggcc gtgaaattga ggtgattgac cttgttcaga
      781 tccccgtgag gtgcaatgcc cgtgtcgatg acgtagacgt tgaccccatt gaccgcgccc
      841 gagccgtttc cggccagggt ggatgacagg gtggcacggg tggtgctgat gccccacggg
      901 atggtctgtc cgacgatctg caccggaaga tcctcttcga tgctcatcac ggcaggatcg
      961 ctcttcaggc gtgcgagctg ggcatccgtg agcgtggcgg cgaagccttt gagtacgtgg
     1021 ctgaaggcgt ggcgggccac gatgccatgg cggctttcga tgctctgcag gcgttccatc
     1081 acgcgggcgt ccagataagc ccagccggag gggtcactgt tcacccgatg atcacggacg
     1141 agggctcggg ggggcgactg tacggccgtg tcatggaact ggacgatgta gttcctgggg
     1201 gcatcggcct gggccagggg tgctgcgaag gccagggcaa gcagcgggag ggagagggaa
     1261 gggtgcatgg gaggggttcc ggaataatga caaacgtatt ctagggtcct tccgttgcta
     1321 cggccgcggg tcgggcggct tgatgatcat ttgacacact ttgtgacgat ttacacggac
     1381 gcggttcgcg acaacagcgc cgcgctcggg cccacgttgc cggccgccga tgcgccgttc
     1441 agggggtgat gaagggcgtg gcggtatgcc acgtccagca gcgccgggcc agatcaaagc
     1501 tgccgagcgt ttcgagcggt ccgccaatct ccggcggcgc ataggccatc aggcagaccc
     1561 gctcgatgtg cagggtctgc gtgggtagcg ggtgcgcatg gaggcgctcg gcgacggccc
     1621 ccgtcggcca gcagccggcg tagaggccta ccgtgatgtg gggtgtatag gggccgccag
     1681 gatcgctatc gctgtcgtga agggccgtcc gcaggcgggc gatcccgccg tcgggatcgg
     1741 agacgctgat gaacggggcg gacgtgaagc tggcgaggcc gccggtttcg aggaggaagg
     1801 acgtcggcgc gcgccgcgtg agggcggcga gatccgcgtc caggccggcc gcgccaaact
     1861 cgtcatgccg gcgcggcgtg gcggccggaa agccgcgcag ggccagggtg acgtgggcct
     1921 ggcgccgata ggtgtcgagc agcaggtcgg caaggtggcc ctgggcggcg tccaggatcc
     1981 ggcgcacgca tgccgtatcg acgtccagtg cccacagggc ataacggggg cggcccagat
     2041 gccactccac gaaatcccgg cggacgtttt cgagggtcga acccgcggcg gcgaaacctt
     2101 cagcgacggc ggggagggtg ggccaggaca tgcaggactg aaggctgaaa acctgatgat
     2161 cataatgcat cgttgcacgg ggcgagcgta tcgcgcggcc ttccgtccgc aggcccggcg
     2221 tcgggtcgcg ggcggatcgg cgcagccctg cgggaagcgt ccgcatcggg tgcgcgggcg
     2281 cggagcgggt atgcgcggca aatccgctta cagtttcttc cccgccattg tgccggtggg
     2341 cgggcgtctg tccgcacgcc ggccgaccgc ctccggtatc atgttgcacc gcatttcgta
     2401 cgaccgccca tggaactcct cagcaccttc atcgacatcg tcctgcacct ggacgtgcat
     2461 ctggcggccc tgaccgccca gtacggcacc tggatctacg ccatcctgtt cgccatcatc
     2521 ttctgtgaga cggggctggt gatctttccc ttcctgccgg gcgattccct gctctttgtg
     2581 gcgggcgccc tggccgcgac cggcggcatg gacatcacca ccctgtgcgg cgtgctgctg
     2641 gcggcggcgg tgctggggga caacaccaac tactggatcg gccacaccct cggtcccaag
     2701 gtgttccgct gggagaactc ccgcttcttc aatcgcgcgg ccttcgacaa gacccacgcc
     2761 tacttcgaag tccacggcgg caagaccatc atcgtcgccc gcttcctgcc cctgctgcgc
     2821 accttcgcgc cctttgtggc cggcgtggcg cgcatgactt acgcccgctt cgtggccctg
     2881 gacttcatcg gcggctgcgt gtggatcctg tccctcacct ggctcggcta cggcttcggc
     2941 aacctgcccg aggtgaagaa gaacctgtcc ctggtgatcg tgggcatcat cgccctgtcc
     3001 ctgatgccgg tggtggtcgg ctggctgcgc ggtcgcagtg cgcgggcggc gaactgaggc
     3061 ggcggcatga agaatatcgt catcctcatc tccgggcgcg gctccaacat ggaggccatc
     3121 gtgcgggcgc gtatcgacgg cgcgcggatc gccgcggtca tcgccaaccg gcccgacgcg
     3181 gcgggcctgc agtttgccgc acacgccggc atcgccaccc gcgtggtgga tcacaaggcc
     3241 tttgccagcc gcgaagcctt cgacgcggcc ctggccgacg tcatcgacgg cttcgccccc
     3301 gatctggtgg tgctggcggg cttcatgcgc gtgctcaccg atggcttcgt ccagcgctat
     3361 accggccgcc tcctcaacat ccacccctcg ctgctgccgt ccttccctgg tctgcacacc
     3421 caccagcggg cgctggaagc cggcgtgcgc gtgcatggca ccacggtgca tttcgtgacg
     3481 cccgcgctcg actgcggccc catcgtcatc caggccgcgg tgccggtacg ccccggcgac
     3541 gacgaagacc gcctcgccgc gcgggtgctg gtgcaggagc accgcatcta cccgcaggcg
     3601 gtgcgctggt tcgtcgaagg ccgcctcagc ctcacgccgg cgggtacggt ccaggtcgcc
     3661 gatgtggcgg acggccccga cggctggacc gtgccgcccg tcgagggctg agactgccgg
     3721 atggccgtcg gtcggatccg caaccgggcc ctcgcgatcg ccgtggccct gtccctgctg
     3781 gtgcatgggc tggcgatcct gagtccggcc tggcgtctgc cctcggacga cgccaccgtg
     3841 ctcccgccgc tggacgcgcg cctcacgccc ctgccgccgc cggttctcca gccttccgac
     3901 gagaaacgtc atctgctggg cgaggcgcca ccgccgcggc cccgtccgcg tccgcaggcg
     3961 cgccccccgg cgctgcccgc cgggcccgtc gcggagctgc cggccgatcc tgccggtgcg
     4021 ccggagctgg ccggcccggc cagcaacgac tctgcggcgc cgcccacccc ggactccgcc
     4081 accgaagccc cgcccgcgac gcccgtcgaa ccgaatgccg ccgacgtgcc ggccggcggc
     4141 gccgacctgg gcgagctgtg ggccgcctcc ggcaaggtgc aatacaacgt gatccgcggc
     4201 gagcgcaact tcatcatcgg ccgcaccacc cacgcgtgga cccatgacaa ggagcactac
     4261 tccatggaga cggtgatcga gaccgtcggt ctggtggggc tcatcaaacc cttccgcatg
     4321 gtccagcgca gcgagggccg catcggcgcc cacggcctgt tgccggacaa atttaccgtc
     4381 gagcgggacg ggcaggtcaa ggagcgcgcc gatttcgact ggtccaccgc caaggtcgtg
     4441 ctgatggccg gcgaccggcg gcgggaattc gatctggccg aaggcgacca ggacgtgctg
     4501 tccctgatgc atcaactctc cctccagccg gacgggccga tgaacgccga gctgctggtc
     4561 gtcaccggca agagcgcggt ccgttcggcg atccgcaatc tcggcctgga agacctggaa
     4621 gtccccgcgg gccgggtttc ggcctaccat ctgggcagca ccggccacaa cggcgagctc
     4681 aagatcgaca tctggctggc caaggattac gccaacctgc cggtccggct caagatcacc
     4741 gacaagaacg gcgacgtcct cgaccaggtc gcgacgggcg tcgacctcgg ccctcgcgcc
     4801 gataaaaagt aaaatccccc ctttcccggc tttgtccgtc ccgccgccgc ggcgggaccg
     4861 ctcctttcag tatcggaagt ccatgaccat caccgcctcc cagatccagt ccgccagcca
     4921 ggccctgcgt gccgtgctgc gcttcgatta ccccgccgac gcggtcctgt cccgctattt
     4981 ccgcgacctg cgcgaactgg gcgcccgcga tcgggccttc gtcgccgagg cggtgtatgg
     5041 cgtgctgcgc cgcaagcgca tgctggaacg cctgtgcggg ccggaagtct cgccccgcca
     5101 actggtgctg gcctggctcg cccgcgtcga aggcctcacg ttgcgccagc tcggtgaagg
     5161 cctgcacggc cgcgataccg acttcttcac cgccatcaag ggtcgtgaac tgcccgaagg
     5221 cagcctggcc gaaaccaccg acctgcccga atggctggcc gaacgcctcc tcgccacgcg
     5281 caccccggag caggtgcagg ccctcgccca gagcctcaac cggcctgcgc cgctggacct
     5341 gcgggtcaat cccctcaagc tcgaccggga cgccctcatc gaaaaactgc gcgccgacgg
     5401 catcgcctgc gccgccgcgc ccctgtcgcc gctggccgtg cgcctggaag gcaagcccgc
     5461 gctgcagaag catccgctct tcctcgacgg cagtttcgag gtgcaggacg aaggcagcca
     5521 gctgctgggc ttcctggtgc agcccaagcg cggcgagatg gtcgtggatt tctgcgccgg
     5581 cgccggcggc aagaccctgc tgctgggcgc gctgatgcgc aactccgggc gcctctatgc
     5641 cttcgacgtg tccgacaagc gcctcgccaa actcaagccg cgggtggcgc gctccggcct
     5701 gtccaacgtc catccggtgg tcatcgccag cgagaacgat ccccgcgtca agcgcctcgc
     5761 cggcaagatc gaccgggtgc tggtggacgc gccgtgcagc ggcctcggca ccctgcgccg
     5821 caacccggac ctcaaatggc gtcagagccc cgagtccgtc gccgagctca ccgccaaaca
     5881 ggccgccatc ctggccagcg cggcgcgtct ggtgaaggcg ggcggccgcc tggtctacgc
     5941 cacctgcagc ctgctgccag aggagaacga agccatcgtc gcggccttcc tggcggccca
     6001 tccgggcttc gtgcaggtgt cggccggcga gatcctgggt aagcagggca tcaccgtggg
     6061 cgacgccgag cagctccacc tgagcccggc cgagcacgac accgacggct tcttcgccgc
     6121 ggtgctggag cgcagcgccg gatgatcggc cgcggcgccc aggcgctcct gctggcctgc
     6181 gcggtcgcca cggccgacgc ccagacgccg ggcgtcgagc ccacccgcac ggtggcgatg
     6241 cacggcaccg ccgaagttgc ttttgccccg tggagcgatc ccgaggcgct gctgctccag
     6301 gccatcgacg acgcccggga ttccatccac gtgcaggcct atctcttcac cagcaaggcg
     6361 ctggcgcggg gcctgatcgc agcccggcgg cgcggcctgc gggtcgaggt gctgctcgac
     6421 gccgagatga accgccccgg ctccctcagc gtgctgcctg agctgctgga ggccggcgtg
     6481 ccggtggcga tgctcacgcg cttcaacatc gcccacaaca aggtgatgct cttcgacgcc
     6541 gacacgcccc acgcggcgct ggccaccggc tcctacaact acacccgctc tgcgcgcctg
     6601 agcaacgcgg agaacctgct gatcctgcgc ggcaaccccg cgctggtccg cctttacctg
     6661 gatacctggc agcgccagcg cgccgaggcc gtgcccctgc gcagcctcga cgacctgccc
     6721 gcacggaagg acaaacggga tggaagagaa tcagatcgca caactgttga ttgacacctg
     6781 ggccgacctg caggagcccg ccatcttctg gcaggtgggc gtgctggccc tgtgtctgct
     6841 gctggccggg ctcatttccc ggcagctgcg ccagatcatc gcccggcgcc gctccgtgcg
     6901 ggacgacgct gctgccgccg gggccggcga agagggcctg cggcgggtca tctttccact
     6961 caccgcgctg gtgctggtgc tcctcgcgcg catcgtgctg cacaagtggc atcacgtgaa
     7021 tctgctcaag ctggcggtgc ccctgctgct ggcgggggcg gcgatccggc tcatcgtgca
     7081 tgccctcaag cgcgccttcc cccaggccgg ctggctcgcg tccttcgagc ggatcttctc
     7141 caccctggcg tggctcgcgg tggccctgca catcaccggc gtgctgccca ccgtcatcga
     7201 cgggctcgag cagatcgggt tcagcatcgg caccgcgcac gtcaatctgt ggatgctctt
     7261 ccagggcgcc ctcacggtgc tcctcaccgt cctcgtggcc ctgtgggtgt ccggctccat
     7321 cgaaagccgc ctgatggcca cggaggggct cgacgccaac ctcaagctgg tctttgcccg
     7381 cctcgccaag gccttgctgc tggtgctggc ggtgaccatc ggcctgcccc tggtgggcat
     7441 cgacctcacc gcgctttcgg tgttcggcgg cgccctcggc gtgggcctgg gcttcggcat
     7501 gcagaagatc gcggccaact acgtgtccgg cttcatcctc ctgctggacc gctcgatccg
     7561 cctgggcaac ctcatctcgg tgggcaccga gcggggcgtg gtcagccaga tcaccacgcg
     7621 ctacacggtg ctcaagggca tgaccggcat cgaatccatc gtccccaacg agaccctggt
     7681 gggctccgtg gtgcagaacg aaaccttcac cgacccgcgc gtgcgcatcg ccctgcccgt
     7741 gcaggtgagc tacgcctgcg atctggaacg cgccctcacg atcctggtcg aatctgccaa
     7801 gacccagccg cgggtgctca ccgatccggg gcccggcgcc ttcgtcgaag cctttgccga
     7861 cagcggcatc aacctgcagc tgggcttctg gatcgccgat ccccacgaag gcacgctggg
     7921 catccgctcg gccatcaatc tggagatctg gcggcgcttc aaggccgagg gcatcgagat
     7981 tccctttccc cagcgggagg tgcgtctgct caatccgcca gcgccggatg tggcccctcc
     8041 gccacaatcg cccgcctgag cgagcgctcg gcagggcttt tttcgggtct atattggatt
     8101 ggcggagccc ctcgcttaga atcgcgccca cgctcgaata taaggatgta ttgacatgct
     8161 gtcttccggt ctgatcgatc tgccctggtg gggatacatc gtcgtcacgc tggccctgac
     8221 ccacgtcacc attgcctcgg tgacgatctt cctgcaccgc catcaggccc accgcgccct
     8281 ggaactgcac ccgctgccgg ccctgttctt ccgcacctgg ctgtggctca ccaccggcat
     8341 ggtcaccaag gaatgggctg ccatccaccg caagcaccac gccaagtgcg aaaccccgga
     8401 agatccccac agcccgcaga tcgtcggcat caacaaggtg ctgtggaccg gcgtcttcct
     8461 ctacgtcaag gaagcccgca acaaggacac cctgcagcgc tacggccacg gcacgcccga
     8521 tgactggttc gagcgccaca tcttctctgc cgcccccaaa ctgggcatcg tcatcaccct
     8581 catcgtcgat tccatcgtct tcggcgtgtg gcccggcgtg ctgatctggg gtgtgcagat
     8641 ggtgtggatc cccttctggg ccgccggcgt catcaacggc ctggggcact tctggggcta
     8701 ccgcaactac aactgtgccg acgcctccac caacctcatc ccgtggggca tcctcatcgg
     8761 cggcgaggaa ctgcacaaca accaccactc ctttgccacc tccgccaagc tgtcggcccg
     8821 ctggtacgag ttcgacatcg gctgggtgta catccgcagc ctcgagatcc tcggcctcgc
     8881 ccgcgtcaag aaggtcatcc ccacgccgcg cttcggcgtc gccaaggccc gcgccgactt
     8941 cgacatgctg caggccgtca tcactcaccg ctacgacgtg atgacccgct acgtgcagtc
     9001 cctcggccag atcgccaagg acgagttcga agccctcaag gcccagtctg gcggcaagct
     9061 caacatcaag gacgtgcgcc gctggctcac cgccgacgaa gcccagctcg gcgccgacga
     9121 gcgccagcgc ctcgccaccc tgctcaagga caatgcccgc ctgcagaccg tggtcgccat
     9181 gcgccaggag ctcaccgccc tgtgggcccg ctccaccgcc acccgcgaac agctcctgca
     9241 gcagctccag gactggatcg cccgcgccga aggcagcggc atccggcaac tcgaagactt
     9301 ctccctgcgc ctgcgccgct acgccgcgtg agccgtccgc ccccgcgcag acctgcacaa
     9361 acccgccggg atggcgggtt tgtcgcttct gagggacgcc gcaacgcccc ccaggccacc
     9421 ccggcccgcc caggcgcttc tccccgcgcg ccgcgcgcgg ccccgccttc cggcggccgc
     9481 ctgatcctcc tcaacaagcc ctacggcgtg ctgtgccagt tcaccgacga agccggccgc
     9541 gccaccctcg ccgactacgt cccggtaccc ggcgtatatg ccgccggccg cctcgacgcc
     9601 gacagcgaag gcctgctcct cctcaccgac gacggcgccc tgcaggcgcg catcgccgac
     9661 ccccgccaca agctgcccaa aacctatctc gtccaggtgg aaggtgtccc cgacgtcacc
     9721 gcgctcgaat ccctgcgccg tggcgtcgac ctgggcgact ttgtcacccg cccgtgcagc
     9781 gcccgcctcg tgcccgagcc cgactggctg tggccccgcg acccgcccat ccgcgaacgc
     9841 aaagccatcc ccgtgagctg gctggaaatc gtcctgcgcg aaggcaagaa ccgccaggtg
     9901 cgccgcatga ccgccaaggt cggacatccc acgctgcgcc tcatccgcgt cgccatcggc
     9961 ccgtggacac tgggaaacct cacgcccggt gcatggcgcg atgacacgat tcgggaaaaa
    10021 tctgttaaaa acacctgacc ggtacggcct ggcccgatcc gtcagggagg gagcgaagcc
    10081 catccgttct ccatactcat aaatctgccc aaggaatcat catgaagaag ctgttgtccg
    10141 ccctggtggc cggcctgttc gcggcgacca ccgccgtctc cgccgccgac ctgcccgcca
    10201 cccccgctgc cgcccccgct gccgtgagcg cccccgccac cccggctccc acggtgaaga
    10261 aggccaagaa ggccaagaag tcgaagaaga gcagcgcagc caaggccgag aagaaggcca
    10321 agaaagacaa gaaggccggc aaggacaaga agcacaagaa gcataaaaag cacaagaagg
    10381 cggcggtctg aggcagccac gctcggagtg aaagcggcgg gggaatcccg ccgtttttat
    10441 tttgaaaaat cgaatttgct gctgggtcta tccccgcgtc cgcaggtgaa gaacttgcgc
    10501 tgctgaagct tcgaagaggc cggggcaggc gccccggaac gacaaccttc acgaaggcca
    10561 tgatcaccga catcaaggtg cttcaatgag gccggggcgg gagccccgga acatcaagaa
    10621 cagcgccgac acatacgccg gcgctagata cgcttcaatg aggccggggc gggagccccg
    10681 gaacatcgcg atgtgctccg gggtgacctt gaaagcgccc tccggcttca atgaggccgg
    10741 ggcgggagcc ccggaacatc atggaccatt ccgatgctcg tcccgcccat cggcacgctt
    10801 caatgaggcc ggggcgggag ccccggaaca tctacacagg ctcgttgaga tggtgcgtgc
    10861 cgtccatgct tcaatgaggc cggggcggga gccccggaac atctagagcg cgcgaaagct
    10921 gcaatcggtg acgaaggggc ttcaatgagg ccggggcggg agccccggaa catcgccacc
    10981 tcgccggaag cggaatccat cctgggcttg cttcaatgag gccggggcgg gagccccgta
    11041 acatccgcga ttgataccgc gtatggcgcc ttgatcggcg ggcttcaatg aggccggggc
    11101 gggagccccg gaacatcctc catggagacg gcgtcggcgg tcttgatcgc gttggtgctt
    11161 caatgaggcc ggggcgggag ccccggaaca tcactgtcgt agtaggccgc cgcggcgata
    11221 tcgccgatgc ttcaatgagg ccggggcggg agccccggaa catcgggggc caccaccagc
    11281 aagccgtccg gcactgggtc cggcttcaat gaggccgggg cgggagcccc ggaacatccc
    11341 cgatacggct acaaggaagg ggtggcggca tgaggcttca atgaggccgg ggcgggagcc
    11401 ccggaacatc gatgcggtgg ggccgctggt ctgtaaagag agagctagct tcaatgaggc
    11461 cggggcggga gccccggaac atcccatgtc cgtaatcaag aaagccgcga tccacatcaa
    11521 ggcttcaatg aggccggggc gggagccccg gaacatctca ggacgaaatc tcatccgggc
    11581 tgcgctacct caagcttcaa tgaggccggg gcgggagccc cggaacatct gaggcggatt
    11641 cgagcgggga ggttgaatga aatcgcttca atgaggccgg ggcgggagcc ccggaacatc
    11701 atgacctgaa ttccgcgtac ccctacgtga tggcgcttca atgaggccgg ggcgggagcc
    11761 ccggaacatc
//
LOCUS       DAOQMM010000113        11499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2559_length_11499_cov_54.691367, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000113 DAOQMM010000000
VERSION     DAOQMM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2559_length_11499_cov_54.691367"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..995
                     /locus_tag="PLH85_14750"
     CDS             69..995
                     /locus_tag="PLH85_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNB65875.1"
                     /translation="MPRIRILATGGTIAGASDPAGDGARYVAGVVDVDRLVGQVTGLS
                     ALAQVDAAQVLALDSKDMTPGHWQVLRPRICDALRDPGVDGVVILHGTDTLEETAWFL
                     HLTLPADKPVVLTGAMRPADHPQADGPANIVAAVSHAAAGAPGVRIVLNGGPWQAREL
                     VKARTLGLDAFSAGASTAPLRSAGCFAHLDNAALPRVDILPGYAGAPADLIDACVTAG
                     ARGLVLALAGHGSVPAAWVPALRRARNAGVRLLRASRCAGPVVHNGNADDDGEGYLTA
                     GDLPPLKARIALMLALADGMSFHATGERLATL"
     gene            complement(1032..2816)
                     /locus_tag="PLH85_14755"
     CDS             complement(1032..2816)
                     /locus_tag="PLH85_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNB65876.1"
                     /translation="MQSLTTFAALLLATVLFVPWFKRAGLGTVLGYLAVGMLIGPSGI
                     KLVRDPDNLLHTAELGVVLLLFVIGLELKPSRLWALRHSVFGLGALQMAGVAAALAGV
                     GHYFGLSWSAASLVGIILALSSTAFVLPTLAERRELTTRYGRESFAILLFQDLSVIPL
                     LALIPLFGTGRPTDLTHPGVGLVLLVVIVLVGRRVLDALFAHVARVNSREMFTAAALV
                     TVLGLALIMQAAGLSMSLGAFVAGVLLADSEFRHELEAAIAPFQGLLLGLFFIAVGMS
                     TNLQLLVDQPGRIIGLTVGLVAMKAVVMYVLRRVLGGSKGDARMLALALAQGGEFAFV
                     LFDVAQAHRILGEVQADRLTLVVALSMAVSPLLLMLGDWLARLEAAREPARPFDELPD
                     ESSEVVIAGFGRVGQIIGRLLVARGVRFTALDADADQIDTVRRFGFKVFYGDAANLDL
                     LHAAQVGSAKVFVLAIDNVEASLRTAELVRRHFPDVEVVARARNRFHANRLMDLGIQR
                     QIRETLPASLDMARWVLETLDDPPDLAAQLVARFARHDADLLARQQAISHDEKQLIQS
                     SRDARAELAQILEDARLAFAGKGKTRGS"
     gene            3120..4271
                     /locus_tag="PLH85_14760"
     CDS             3120..4271
                     /locus_tag="PLH85_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65877.1"
                     /translation="MKTHRLSAASASAILTACLAATAHAGPIAIYDGPAIDKTSDACA
                     SFGDAISCSLPYLNFLDGLAQGTTTSAGGYVLQSSQGLLKDSIVIQAGGNAALDNSDT
                     SPAPAVVENGFKSNSGSQHYLATGKTDGTTMSAGNMSDPTNNGLTASQDKIGTWDVSA
                     VWLRDALTTADGKRHELMIAFDYNQPQASATSMDIWSLITVRKSDGGLPDIEFEFNGD
                     TSGYASFSSSKNFYSQPSAADFDTVSGTICLYDNNQALPYPGGSCPKTYLGGSLVTEI
                     DNSQGTDRTEFLAFLPELNSNLENYIKQGYDTISVRLLMGCFGGTDPDNGQGYLSAGQ
                     TTNCDTGGFGDVYIMGGAAMNRIPEPSSLSLAVLALAGWMGRALPLRRT"
     gene            complement(4296..5237)
                     /gene="cysB"
                     /locus_tag="PLH85_14765"
     CDS             complement(4296..5237)
                     /gene="cysB"
                     /locus_tag="PLH85_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNB65878.1"
                     /translation="MNIQQLRYVFEVARHRLNVSEAAERLFTSQPGVSKQIRLLEDEL
                     GIEIFVRHGKRLVGITEPGRQVLSIAGRILQEVDNLREVGAEFSNEATGSLSIAFTHT
                     QARYALPRVVQAFMQRYPKVKLSFHQGNPRQVCEYVLSGEADIAIATEAIAEHEDIVM
                     LPCYQWNRCIIAPPRHPILSEQPLTLEAIARYPLVTYDFAFTGRNRINEAFTGRGLKP
                     NVVLTAIDSDVIKTYVAMGLGVGILARMAYDPAVDKDLVMMDASHLFASSTTRIGLRR
                     NAWLRGYVYAFIELFAPHLSRRVVDAALAGGGEDPGL"
     gene            5357..6136
                     /locus_tag="PLH85_14770"
     CDS             5357..6136
                     /locus_tag="PLH85_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB65879.1"
                     /translation="MSIAYTIAGFSVGAIVGLTGVGGGSLMTPLLVLLFGINPSVAVG
                     TDLLYAAITKAGGTLAHGLKGTVDWAVTRRLALGSIPASTVTLLLVGHFAPGGIGGAT
                     GLIKFSLGIALLLTAVAIVFRRQIQNFALAHSSGHEDPRRIALLTTLTGAVLGVLVSI
                     SSVGAGALGVSALFFLYPRLPTLRIIGSDIAHAVPLTLVAGIGHWFLGSVDWALLGSL
                     LIGSLPGIWLGSHVSTRVPDRVLRPILATMLVLVGSKLIVA"
     gene            6165..7847
                     /locus_tag="PLH85_14775"
     CDS             6165..7847
                     /locus_tag="PLH85_14775"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNB65880.1"
                     /translation="MYRYDSYDQRIVDERVAQFREQTRRYLAGELTDDEFRPLRLQNG
                     LYVQRHAPMLRVAVPYGLLSSAQVRTLAHIARTYDKGYAHFTTRQNVQFNWPDLKVVP
                     EILAELATVQMHAIQTSGACIRNITSDPFAGVAGDELIDPRPWCEILRQWSTFHPEFA
                     FLPRKFKIAVNGAEEDRAVIQCHDIGLELKRDAAGQIGFRVLVGGGLGRTPIIGEEIS
                     AYVPWQHIITYCEAILRVYNRHGRRDNLYKARIKILVKALGIEEFRRQVEAEWLHTKD
                     GPSTLTEAEVARVAGHFTTPAYAALTAEDAVFAARRADTPAFAAWVRRNVRPHKVPGY
                     AIAILSLKHPKSPPGDITAEQLDLVASWADAYSFGELRATHEQNLVLADVKQSDLFTL
                     WEQARDAGLATPTFGLLTDIICCPGGDFCALANAKSIPIANALQARFDDLDYLHDIGE
                     LELNISGCMNSCGHHHVGHIGILGVDKNGEEWYQVSLGGSQGKRTSLGKVIGRSFAAA
                     EMPDVIGKILDVYVAQRQTAEAFIDTVRRIGLDPFKLGVYGEAAVKERKQHV"
     gene            7840..8364
                     /locus_tag="PLH85_14780"
     CDS             7840..8364
                     /locus_tag="PLH85_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNB65881.1"
                     /translation="MSKLIKNGRLSDDAAQILVLDETQAPEALTLPDGPVFVPLAVWQ
                     AHKGVLASRSDVGVWLDSHERVEELADDLGTLSAIALNFPKFTDGRAYSEAALLRSRY
                     GFTGEIRAIGDVLRDQFFYMQRCGFDAIQPKAGKYSDVQLEAALASLNDFSEPYQAAV
                     DHAAPLFRRAQRSA"
     gene            8378..9103
                     /locus_tag="PLH85_14785"
     CDS             8378..9103
                     /locus_tag="PLH85_14785"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNB65882.1"
                     /translation="MHPNQHPKLDDPALIAALDAKVATLVADLSAWAAELPAITMANS
                     LGAEDMVLTDVIATRGLPIEIFSLDTGRLPLETYDLMAEVQKRYGLKLKFYYPRHELV
                     EAYTRDHGINAFYDSVELRKACCHVRKVEPLGRALAGKQAWITGLRAQQSTTRTDLPK
                     RQFDEGNGLVKFNPLSDWSEKEVWAYIRIHQVPYNALHDKFYPSIGCAPCTRPISLGE
                     DIRAGRWWWENPETKECGLHVKK"
     gene            9139..10074
                     /gene="cysD"
                     /locus_tag="PLH85_14790"
     CDS             9139..10074
                     /gene="cysD"
                     /locus_tag="PLH85_14790"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNB65883.1"
                     /translation="MSTLTQQTLSHLDWLESEAIHIMREVAGQCSNPVLLFSGGKDSI
                     CMLRIAEKAFRPGRFPFPLMHIDTGHNYREVVEFRDRRAAELGERLIVRSVEDSMKRG
                     TVVLKHENESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQW
                     DPKNQRPELWNLYNARSHKGENIRAFPISNWTEMDVWQYIEREELELPSIYFAHTRPV
                     VMRAGAIVPVNVPLMNGELTNLPKPGEEIIERQVRFRTVGDISCTAPVDSDADTVQKI
                     VLETATTTITERGATRLDDQTSEASMEQRKKEGYF"
     gene            10075..11349
                     /gene="cysN"
                     /locus_tag="PLH85_14795"
     CDS             10075..11349
                     /gene="cysN"
                     /locus_tag="PLH85_14795"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNB65884.1"
                     /translation="MNAQHVEDRGLLRFLTCGSVDDGKSTLIGRLLFDTKAILADTLT
                     AIERTSSKRGMTAVDLSLLTDGLQAEREQGITIDVAYRYFSTGSRKYIIADAPGHEQY
                     TRNMVTAASTANLAIILIDARKGVLTQTRRHSYLANLVGIPHLIVAVNKMDLVDYDRA
                     TFERIVGEYRAFAEQIGLTDVRFIPLSALNGDMVVDRGDRLDWYDGPTLIDILEAAPA
                     AHTEKAESFRFPVQYVCRPQASDNPDLHDYRGFMGRVEAGEAKVGDTVTVLPGGRSSR
                     IKDIQLNGQSLPRAIHEQSVTLLLEDEIDISRGDMIVNADEVPAQTKQIEAMLCWMSE
                     TPLSPARTYWLRHTTREVKAKVAGIAYRVDINSLDHESVTTLAMNDIARLTLKLAQPI
                     FADPYVDSRATGAFIIIDEATNNTVGAGMITR"
BASE COUNT         2149 a         4154 c         3379 g         1817 t
ORIGIN      
        1 ccttcttcga gcgccccagg tcggaacggg cccagcagtt cctggcgaag atcctgcagc
       61 actgagccat gccccgcatc cgcatcctcg ccaccggcgg caccattgcc ggtgcatcag
      121 accctgccgg cgacggcgcc cgttacgtgg cgggggttgt cgatgtggac cggcttgtcg
      181 ggcaggtcac aggtctgtcc gcgctggccc aggtggacgc ggcgcaggtg ctggccctcg
      241 acagcaagga catgacgcct ggccactggc aggtgctgcg gccccgcatc tgcgatgccc
      301 tgcgcgaccc cggggtcgac ggtgtggtga tcctccatgg caccgacacg ctcgaagaaa
      361 ccgcctggtt cctgcatctc accctgccgg cagacaagcc ggtggtactg accggcgcca
      421 tgcgccccgc tgaccatccc caggcggacg gacccgccaa catcgtcgcc gccgtgagcc
      481 atgcagccgc gggcgccccc ggcgtgcgga tcgtgctgaa cggcgggccg tggcaggcac
      541 gggagttggt caaggcgcgg acccttggcc tggacgcctt ttcagccgga gcctcgaccg
      601 cgcccctgcg cagcgccggg tgttttgccc atctggacaa cgctgccctg ccgcgggtgg
      661 acatcttgcc gggctacgcc ggcgcaccgg ccgacctgat cgacgcctgc gtgacggcgg
      721 gcgcccgcgg gctggtcctg gcccttgccg ggcatggcag cgtaccggca gcgtgggttc
      781 cggccctgcg ccgggcgcgc aatgcgggcg tccgcctcct gcgggccagt cgctgcgccg
      841 gcccggtcgt ccacaatggc aacgctgacg acgatgggga aggctacctc accgctggtg
      901 acctgccgcc gctcaaggcc cgcatcgccc tgatgctggc gctcgccgac ggcatgtcct
      961 tccacgcgac cggcgagcgc ctcgccaccc tgtaagcccg gcagacggtc cggccgtgac
     1021 gccggccgcg ttcagcttcc gcgggtcttg cccttgcccg caaatgcgag ccgggcgtcc
     1081 tcgagaatct gcgccagctc cgcccgcgca tcgcgggagc tctggatcag ctgcttctcg
     1141 tcatgggaga tggcctgctg gcgcgccagc agatcggcgt cgtggcgggc aaagcgggcc
     1201 accagctggg ccgcaagatc cggcggatcg tccagcgtct ccagcaccca gcgggccatg
     1261 tccagactgg ctggcaaggt ctcgcggatc tgtcgctgga tgcccaggtc catcaagcgg
     1321 ttggcatgaa aacggttgcg cgcccgggcc accacctcca cgtcgggaaa gtgacgacgc
     1381 accagctcgg ccgtacgcag ggacgcctcc acgttgtcga tggccagcac gaagaccttg
     1441 gcggacccca cctgcgccgc gtgaagcagg tcaaggttgg ccgcgtcgcc atagaacacc
     1501 ttgaagccga agcgccgcac cgtgtcaatc tgatccgcgt cggcgtccag ggcggtgaag
     1561 cgcacgccgc gcgccaccag caggcgaccg atgatctgtc cgacccgccc gaagcctgcg
     1621 atcaccacct cgctgctctc gtcgggcagt tcgtcaaaag gccgcgccgg ctcacgcgcc
     1681 gcctccagcc gcgcaagcca gtcgcccagc atcagcagca gcggtgaaac cgccatggac
     1741 aaggccacca cgagggtcag gcgatcggcc tgcacctcgc ccaggatgcg gtgcgcctgg
     1801 gccacgtcga acagcacgaa cgcgaattcc cccccctggg ccagcgccag ggcgagcatc
     1861 cgcgcgtccc ccttgctccc gcccagcaca cgccgcagga cgtacatcac caccgccttc
     1921 atggcgacca gcccgacggt cagtccgatg atgcgtccgg gctgatcgac cagcaactgc
     1981 aggttggtgg acatgcccac ggcgatgaag aacaagccca gcagcagccc ctggaacggc
     2041 gcgatggccg cctccagttc gtggcggaat tcggaatcgg cgagcagcac ccctgcaacg
     2101 aaggcaccca gggacatgga caggcccgcc gcctgcatga tcagggcgag gccgagcacc
     2161 gtcaccagcg ccgcggcggt gaacatctcg cgactgttca cccgcgccac gtgggcaaag
     2221 agcgcatcca acaccctgcg cccgaccagc acgatcacca ccagcaggac gaggccgacg
     2281 ccggggtggg tcaggtcggt gggccggcca gtgccgaaca gggggatcaa ggccagcagg
     2341 ggaatcaccg acagatcctg gaacagcagg atcgcaaagg attcacggcc gtagcgggtg
     2401 gtcagctccc gccgctcggc cagggtcggg agcacgaagg ccgtggatga caaggccagg
     2461 atgatcccca ccagggaggc cgccgaccag gacagcccga agtagtgtcc gacccccgcc
     2521 agcgccgctg cgacgcccgc catctgcaag gcccccagcc cgaacacaga atggcgcagc
     2581 gcccacaggc gggacggctt cagctccaga ccaatgacga acagcagcag caccacaccc
     2641 agctcggctg tgtgcagcag attgtccgga tcccgcacga gcttgatgcc cgaaggaccg
     2701 atgagcatgc ccacggccag ataaccgagc accgtgccca gtccggcgcg cttgaaccag
     2761 ggcacgaaca ggacggtcgc cagcaggagg gccgcgaagg tggtcaggga ttgcatggga
     2821 cgaaaaggcg gggacgaagt ggccaaagtc tctcacaaac gacagccccg ccgcctgcaa
     2881 aagcgtctca aaacacgtca tggcatagat cgccacgcaa caagcatttt taatgttttt
     2941 gtcagatttc tttacacaaa gccactgttc ctcaagatta attttcttga tgccctacga
     3001 aaaccgcatc aaatcgccat tttcagcaaa cgcgaacacc ctggcacaaa taatgcaaat
     3061 ttctgtcaca ccacgccgat tgtccatgtg tggctgaaca aacacccagg agactcgcca
     3121 tgaaaaccca tcgactgagc gccgcatccg cgtccgcaat cctgacggca tgcctggccg
     3181 ccaccgccca cgccggcccc atcgccatct acgatgggcc cgccatcgac aagacttctg
     3241 acgcctgcgc gagttttggc gatgccatca gttgctcgct gccctacctc aacttcctcg
     3301 atgggctggc tcaaggcacc accacctcgg cgggcggcta tgtgctccag tcctcccagg
     3361 gcctgctcaa ggactccatc gtgatccagg caggcggcaa tgccgcgctg gacaacagcg
     3421 acacctcgcc cgcaccggcc gtcgtggaga atggcttcaa gtccaattcg ggcagccagc
     3481 actacctggc caccggcaag accgacggca ccacgatgtc ggccggcaac atgtcggacc
     3541 cgaccaacaa cggtctcacc gccagccagg acaagatcgg aacctgggac gtgagcgccg
     3601 tctggctgcg cgacgcgctc accaccgccg acggcaagcg ccatgaactg atgatcgcct
     3661 tcgactacaa ccagccccag gcgtcggcga catccatgga catctggagc ctgatcaccg
     3721 tccgcaaatc cgatgggggt ctgcctgaca tcgagttcga gttcaatggc gacacctcgg
     3781 gctatgccag cttctcgagc agcaagaact tctacagcca gcccagcgcc gccgacttcg
     3841 atacggtctc cggcacgatc tgcctgtacg acaacaacca ggccctgccc tacccgggcg
     3901 gttcgtgccc gaaaacctat ctgggcggca gtctggtcac cgagatcgac aattcccagg
     3961 ggaccgaccg gaccgagttc ctggccttcc tgccggagct caacagcaac ctcgagaact
     4021 acatcaagca aggctacgac accatttcgg tccgcttgct gatgggctgc ttcggcggaa
     4081 ccgatccgga caacgggcag ggctatctga gcgcgggcca gaccaccaac tgcgacacgg
     4141 gcgggtttgg tgacgtctac atcatgggcg gcgcggccat gaatcgcatc cccgaaccat
     4201 catccctgtc actggccgtg ctggcgctgg ccggctggat ggggcgcgcg ctgccgctac
     4261 gccgcacctg aacgggcccg ggatccaggc tgagctcaca ggcctggatc ctcgccccca
     4321 cccgccagcg ccgcatccac cacgcgccgg gacaggtgtg gcgcaaacag ctcgatgaag
     4381 gcgtacacat agccccgcag ccacgcattg cggcgcagac cgatacgcgt ggtgctggac
     4441 gcaaacaggt gtgaggcgtc catcatgacc aagtctttgt ccaccgccgg gtcgtaggcc
     4501 atgcgcgcca ggatgccgac gccgagcccc atggccacat aggtcttgat cacgtccgaa
     4561 tcgatggcgg tgagcaccac gttgggcttc agtccgcggc cggtgaaggc ctcgttgatg
     4621 cggttgcgcc cggtgaaggc gaaatcgtag gtcaccagcg ggtagcgggc gatcgcctcc
     4681 agcgtcagag gctgttcgga caggatggga tggcgcggcg gggcgatgat gcagcggttc
     4741 cactgatagc agggcagcat cacgatgtcc tcgtgctcgg cgatggcttc ggtggcgatg
     4801 gcgatgtccg cctcgccaga cagcacgtat tcgcacacct ggcgcggatt gccctggtga
     4861 aaggacagtt tgaccttcgg ataccgttgc atgaaggcct gcaccacccg cggcagggca
     4921 tagcgcgcct gggtatgggt aaaggcaatg gacaggctgc cggtggcctc gttgctgaat
     4981 tccgcgccga cctcgcgcag attgtccact tcctgcaaaa tgcggcccgc aatgctcaac
     5041 acctgccggc cgggttcggt gatgcccacc aggcgcttgc cgtgacgcac gaagatctcg
     5101 atgcccaact cgtcctccag caggcggatc tgcttggaca cgccgggctg ggaggtgaac
     5161 agacgctccg cggcttccga cacgttcagc cgatgccggg ccacctcgaa gacatagcgc
     5221 agttgctgga tattcatccg ggcctcctgt ccttcggaat acatataact tttttttcta
     5281 taagtctaac ccagaggtcc ttcttggctc tatcggttcc gtttacgatg cgccgcacaa
     5341 ggaatctgga atcatcatga gcattgccta caccatcgcg ggtttttctg ttggcgccat
     5401 tgtcggtctc accggcgtgg gcggcggctc gctgatgaca ccgctgctgg tgctgctgtt
     5461 cggcatcaat ccctcggtcg ccgtcggcac cgacctgctc tatgccgcca tcaccaaggc
     5521 cggcggcacc cttgcccatg gcctcaaggg cacggtggac tgggccgtca cacggcgcct
     5581 tgccctaggc agcattcctg cgtcgacggt caccctgctg ctcgtcggcc actttgcgcc
     5641 cggcggcatc ggcggcgcca ccgggctcat caaattctcc ctgggcattg ccctgctgct
     5701 caccgcggtc gccatcgtct tccgcaggca gatccagaac ttcgccctcg cccactcctc
     5761 gggccatgaa gacccccgcc ggatcgccct gctcaccacc ctgaccggtg cggtgctggg
     5821 tgtcctggtg tcgatttctt cggtgggcgc cggcgccctg ggcgtcagcg ccctgttctt
     5881 cctgtatcct cgcctgccga ccctgcgcat catcggcagc gacatcgccc acgccgtgcc
     5941 gctgacactc gtagccggca ttggccactg gtttctcggc agcgtggact gggccctcct
     6001 cggcagcctg ctgatcggct ccctgcccgg catctggctg gggagccacg tttccacccg
     6061 ggtgcccgac cgggtgctgc gccccatcct ggccaccatg ctggtattgg tcggcagcaa
     6121 actgatcgtc gcctgatccg gcggcgcccc ctccaggact aatcatgtac cgctacgact
     6181 cctacgatca gagaatcgtc gacgaacgcg tcgcccagtt ccgcgagcag acccgccgct
     6241 acctggcagg cgaactgacc gacgatgaat tccgccccct gcgtctgcag aacggcctgt
     6301 acgtgcagcg ccatgcgccc atgctgcggg tcgccgtccc ctacggcctg ctctcctcgg
     6361 cgcaagtgcg cacgctggcc cacatcgccc gcacctacga caagggctac gcccacttca
     6421 ccacgcgcca gaacgtgcag ttcaactggc cagacctgaa ggtggtgccc gagatcctcg
     6481 ccgagcttgc caccgtgcag atgcacgcga tccagacctc cggcgcgtgc atccgcaaca
     6541 tcacctccga ccccttcgcc ggcgtggcgg gcgatgagct catcgacccg cggccgtggt
     6601 gcgagatcct gcgtcagtgg agcaccttcc acccggaatt cgccttcctg ccgcgcaagt
     6661 tcaagatcgc cgtgaacggc gccgaggaag accgcgccgt catccagtgc cacgacatcg
     6721 ggctggaact gaaacgtgac gccgccggcc agatcggctt ccgcgtgctg gtgggcggtg
     6781 gcctcggacg caccccgatc atcggtgaag agatcagcgc ctacgtaccc tggcagcaca
     6841 tcatcaccta ctgcgaagcc atcctgcgcg tctacaaccg ccacgggcgc cgcgacaacc
     6901 tctacaaggc gcgcatcaag atcctcgtga aggcgctggg catcgaggaa ttccgccgcc
     6961 aggtcgaggc cgaatggctc cacacgaagg acggtccgag taccctcacc gaagcggaag
     7021 tggcgcgcgt cgccggccat ttcaccaccc cggcctatgc cgccctgacc gcggaagacg
     7081 ctgttttcgc cgcccgccgt gcggatacgc cggccttcgc agcgtgggtg cgccgcaacg
     7141 tgcggcccca caaggtgccg ggctacgcca tcgccattct gtccctcaag catccgaaga
     7201 gcccgccggg cgacatcacc gccgagcagc tcgatctcgt cgccagttgg gccgacgcct
     7261 acagcttcgg cgaactgcgc gccacccacg agcagaatct ggtgctggcc gacgtgaagc
     7321 agtccgacct cttcaccctg tgggagcagg cccgcgacgc cggcctcgcc acgcccacct
     7381 tcggcctgct caccgacatc atctgctgcc ccggcggcga tttctgcgcc ctggccaacg
     7441 ccaagtccat ccccatcgcc aacgccctcc aggcccgatt cgacgacctg gactacctcc
     7501 acgacatcgg tgagctggag ctcaacatct ccggctgcat gaactcctgc ggccaccacc
     7561 acgtgggcca catcggcatc ctcggcgtcg acaagaacgg tgaggagtgg taccaggtca
     7621 gcctgggcgg cagccagggc aagcgcacca gcctcggcaa ggtgatcggc cgctccttcg
     7681 ccgctgccga gatgcccgac gtgatcggca agatcctcga cgtctacgta gctcagcgcc
     7741 agaccgccga agccttcatc gacaccgtgc gccgcatcgg cctcgatccc ttcaagctgg
     7801 gcgtctatgg cgaagccgcc gtcaaggaaa ggaagcagca tgtctaagct catcaagaac
     7861 ggccgtctga gcgacgacgc cgcccagatc ctggtcctcg acgagaccca ggctcccgaa
     7921 gccctgacgc tgccggacgg ccccgtcttt gtgcccctcg ccgtgtggca ggcccacaag
     7981 ggcgtcctcg cctcacggtc ggacgtgggc gtctggctcg acagccacga gcgggtggaa
     8041 gagctcgccg acgatttggg aacgctctcc gccatcgccc tcaactttcc caagttcacc
     8101 gacggacggg cctactcgga agccgccctg ctgcgcagcc gctatggctt caccggcgag
     8161 atccgcgcca tcggcgacgt gctgcgcgac cagttcttct acatgcagcg ctgcggcttc
     8221 gacgcaatcc agccgaaggc aggcaagtac tccgatgtcc agctcgaggc cgccctcgcc
     8281 agcctgaacg acttttccga accctaccag gccgccgtgg atcacgccgc gccgctgttc
     8341 cgccgcgccc agcgcagcgc ctgattcagg agcctccatg cacccgaacc agcatcccaa
     8401 gctcgacgat cccgccctga tcgccgccct cgacgccaag gtcgccaccc tggtggccga
     8461 cctgtctgcg tgggctgccg aactgccggc catcacgatg gccaacagcc tgggcgccga
     8521 ggacatggtc ctcaccgacg tgatcgccac ccgcggcctg cccatcgaaa tcttctccct
     8581 cgacaccggc cgcctgcctc tcgaaaccta cgacctgatg gccgaggttc agaagcgcta
     8641 cggcctcaag ctcaagttct actatccccg ccacgagctc gtcgaggcct acacccgcga
     8701 ccacggcatc aacgccttct acgacagcgt cgagctgcgc aaggcctgct gccacgtgcg
     8761 caaggtcgaa cccctcggtc gcgccctcgc cggcaagcag gcctggatca ccggcctgcg
     8821 cgcccagcaa tccaccaccc gcaccgacct gcccaagcgc cagttcgacg agggcaacgg
     8881 cctcgtcaag ttcaatcccc tgagcgactg gagcgagaag gaggtgtggg cctacatccg
     8941 catccatcag gtgccttaca acgccctgca cgacaagttc taccccagca tcggctgcgc
     9001 gccgtgcacg cgcccgattt ccctcggcga agacattcgc gccgggcgct ggtggtggga
     9061 aaatccggaa accaaggaat gcggcctgca cgtcaagaag tgaccctggc cgcctggaat
     9121 cattgaccga gtaaagcaat gtcgacgctc acccaacaaa ccctgagcca cctggactgg
     9181 ctcgaatccg aagccatcca catcatgcgc gaggtggccg gccagtgcag caatcccgtg
     9241 ctgctcttct ccggcggcaa ggactccatc tgcatgctgc gcatcgccga aaaggccttc
     9301 cggcccggcc gcttcccctt cccgctgatg cacatcgaca ccggccacaa ttaccgtgag
     9361 gtcgtcgaat tccgcgacag gcgcgccgcc gaactgggcg agcgtctcat cgtgcgctcg
     9421 gtggaagact ccatgaagcg cggcacggtc gtcctcaagc acgagaacga atcgcgcaac
     9481 aagcaccagt ccgtgacatt gctcgaagcc atcgaggagt tcggcttcga cgcctgcatc
     9541 ggtggcgccc gtcgggacga ggaaaaggcc cgcgccaagg aacgcatcat gagtttccgc
     9601 gacgagttcg gccaatggga cccgaagaac cagcgccccg agttgtggaa cctctacaac
     9661 gcccgcagcc acaagggcga gaacatccgc gccttcccca tctccaactg gaccgagatg
     9721 gacgtctggc agtacatcga gcgcgaggaa ctggaactgc ccagcatcta cttcgcccac
     9781 acccgccctg tggtgatgcg cgccggcgcc atcgtgccgg ttaacgtgcc gctgatgaac
     9841 ggtgaactga ccaacctgcc caagcccggt gaagagatca tcgaacgcca ggtgcgcttc
     9901 cgcaccgtgg gcgacatctc ctgtaccgcg ccggtggatt ccgatgccga cacggtccag
     9961 aagatcgtcc tcgagaccgc caccaccacc atcaccgagc gcggcgcgac ccgtctggac
    10021 gaccagacca gcgaagcctc catggaacaa cgcaagaaag agggttactt ctaaatgaac
    10081 gcccaacacg ttgaggaccg cggcctgctg cgattcctga cctgcggctc ggtcgatgac
    10141 ggcaagagca ccctgatcgg ccgtctgctg ttcgacacca aggccatcct cgccgacacc
    10201 ctcaccgcca tcgaacgcac ctccagcaag cgcggcatga ccgccgtgga cctttccctg
    10261 ctgacggacg gccttcaggc ggaacgcgaa cagggcatca ccatcgacgt ggcctaccgc
    10321 tacttctcca ccggctcacg caagtacatc atcgccgacg caccgggcca cgagcagtac
    10381 acgcgcaaca tggtcaccgc ggcctccact gccaacctcg cgatcatcct catcgacgcg
    10441 cgcaagggcg tgctcaccca gacccgccgc cacagctatc ttgccaacct ggtgggaatc
    10501 ccccacctga tcgtcgccgt gaacaagatg gatctggtgg actacgatcg cgccaccttc
    10561 gagcgcatcg tgggtgaata ccgcgccttc gccgaacaga tcggcctcac agatgtgcgc
    10621 ttcatccccc tgtccgcgct caacggcgac atggtggtgg atcggggcga tcgcctggac
    10681 tggtacgacg gtccaaccct catcgacatc ctcgaagccg cccccgccgc ccacaccgag
    10741 aaggccgaaa gttttcgctt tcccgtccag tacgtgtgcc gtccgcaagc ctcggacaac
    10801 cccgacctgc atgactaccg cggcttcatg ggacgcgtcg aggccggcga ggccaaggtc
    10861 ggtgataccg tcaccgtcct gccaggcggc cggagttcac gcatcaagga catccagctc
    10921 aacggtcagt ccctgccgcg cgccatccac gaacaatccg tgacgctcct cctcgaggac
    10981 gagatcgaca tctcccgtgg cgacatgatc gtcaacgccg acgaggttcc cgcgcagacc
    11041 aaacagatcg aagcgatgct gtgctggatg tctgaaacgc ccctgtcccc tgcccgcacc
    11101 tactggttgc gccatacgac acgggaggtc aaggccaagg ttgcgggcat cgcctaccgg
    11161 gtcgacatca acagcctcga ccacgagtcg gtcaccaccc tggccatgaa cgacatcgcg
    11221 cgcctcaccc tcaagctcgc ccagccgatc tttgccgatc cctatgtgga ctcccgcgcc
    11281 acaggcgcat tcatcatcat cgacgaagcc accaacaaca ccgtcggcgc cggcatgatc
    11341 acgcgctgac aatcgcccta ctccacctaa cgaaatggcc ggtacggatc gcaccgtacc
    11401 ggccatttgt tttgtgatcg ccacgaaagc ccccacccct ttcgcgttgt gttctgccag
    11461 ccgtcatcgt ggcccacgaa cccgtatcga tcccccaac
//
LOCUS       DAOQMM010000114        11366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2616_length_11366_cov_52.726019, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000114 DAOQMM010000000
VERSION     DAOQMM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2616_length_11366_cov_52.726019"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1982
                     /locus_tag="PLH85_14800"
     CDS             <1..1982
                     /locus_tag="PLH85_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB65885.1"
                     /translation="GMWIGTFHGLCNRFLRAHHRDAGLPQLFQILDSGDQLAAIKRLL
                     KTLNVDDEKFPPRDLQHFINGQKEAGIRPPAVEAWDDFTRRKVELYAEYEAQCQRESV
                     VDFPELLLRCYEVLERNEPLRRHYQARFRHILVDEFQDTNRLQYRWLSLLADGGREGG
                     ARVFCVGDDDQSIYRFRGAEIGNMRDFERAFHVTDVIRLEQNYRSHGNILDAANALIA
                     HNRERLGKHLWTDRGAGEPIRVYEAFTDTDEARWIVEEIQSLVRDGVSRSSVALLYRS
                     NAQSRVLEHQLFSNGVAYRVYGGLRFFERQEIKHALAYLRLLANPDDDTAFLRVVNFP
                     TRGIGARSLEALQDAARTWNTPLYQTVPHLSGKPGTALAQFVTLIERLRHETRDLPLH
                     ERVDHVLERSTLRDHYKGEKEGQERLENLDELINAAANFSAEFQVNGASEGAPDTATV
                     LAEFLAHASLEAGDHQADAGSEAVQLMTVHAAKGLEFDVVFLSGLEEGLFPHENSILE
                     SEGLEEERRLMYVAVTRARQRLYLSFTQSRMLHGQVRYNLRSRFLEEIPDGLTKWLTP
                     PNTRHGGFAAESPARYFTPSSARPAAAVMPVSPPLKSRDLGGFAIGQNVRHARFGDGV
                     IVAAEGSGADARVQVKFGAAGSKWLMLSMAKLTAA"
     gene            complement(2041..2808)
                     /locus_tag="PLH85_14805"
     CDS             complement(2041..2808)
                     /locus_tag="PLH85_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNB65886.1"
                     /translation="MRRVVFNQKGGVGKSTITCNLAAISAHQGLRTLVVDLDPQGNST
                     HYLLGHALENADDTLAGLFEQTLAFKFNPRQTAEFVQPTPFPGLSLLPSHPSLEELQS
                     KLESRYKIYKLRDALDELADRFDAVYIDTPPALNFYTRSALIATRRCLIPFDCDDFSR
                     RALYALLENVQEIKADHNRDLEVEGIVVNQYQPRASLPQKLVQELIAEGLPIFKTYLS
                     SSIKIRESHEQAKPMIHLEPRHKLAQEFIALHGELKG"
     gene            3149..5440
                     /locus_tag="PLH85_14810"
     CDS             3149..5440
                     /locus_tag="PLH85_14810"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNB65887.1"
                     /translation="MDKDFIAAALEYHRSPTRGKIAVVPTKGLTNQRDLALAYSPGVA
                     AACDAIVADPADAQEYTSRGNLVAVVTNGTAVLGLGNIGPLAAKPVMEGKGCLFKKFA
                     NIDVFDIELAENDPDKLIDIIAALEPTLGGINLEDIKAPECFYIEKKLRERMNIPVFH
                     DDQHGTAIISAAAMLNGLKVVGKDIGAVKVVCSGAGAAAIACLDLWCSLGIRRENVFV
                     CDSKGVIYVGREENMEPTKARYAQNTAFRTLADAMVGADVFLGLSTAGVLKPEMVKTM
                     ADKPLIFALANPTPEIMPELAKEVRPDAIIATGRSDYPNQVNNVLCFPFIFRGALDVA
                     ATKITDEMKLACVKAIAELAEAEQSDVVAQAYGGADLSFGPNYLIPKPFDPRLIVKIA
                     PAVAQAAIESGVATRAIDMDAYVQSLTEFVYQSGIIMKPVFSKAKGIPADKKRVLYTE
                     GESERVLRAVRAVVDENLARPILIGRPAVIDMRIQKFGLNLQAGRDFDVVNVESDSRY
                     RELWHEYYRLMGRDGVTPDSAREALRSDTTLIGCMLLRRGDADALVCGTTGAYDHHFN
                     HVRNLVGLKKGASLFAAMNMLLLPKRVLAIADTYVNEMPGAEEIAEITVMAAEELKRF
                     GIEPKAALLSHSNFGSAPSASSRKMRAARDLLRVQAPDLQCDGEMHGDSALSAEIRDR
                     LHPDSTLTGEANLLVMPNLDAANIAFNLIKVANGDGVSVGPILLGAALPVHILTPSAS
                     VRRLVNITALAVVDAAEQRVEKV"
     gene            5538..6512
                     /locus_tag="PLH85_14815"
     CDS             5538..6512
                     /locus_tag="PLH85_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HNB65888.1"
                     /translation="MSHQIRFSRTGGPEVLEWCESEPLPPGEGEARVRHHAVGLNYID
                     VYHRSGLYPVPLPSGLGLEAAGEVEAVGPGVTGLKPGDRVAYAGGPLGAYAEVRNLPA
                     DRLVILPDALSYEQGAAMMLQGLTAQYLLRRTYRVQSGDTILIHAAAGGVGLIVCQWA
                     KALGATVIGTVGSDDKAALARAHGCDHAIVYTREKVSERVRALTGGQGVQVVYDSIGR
                     DTFMDSLACLAPLGMMVSFGNASGPVDAFDPGVLARMGSLFLTRPSLFHYAARREQLV
                     AMADELFAVVTSGQVRLSIGQRYALQDVAAAHRDLEARRTTGSTILIP"
     gene            6509..6898
                     /locus_tag="PLH85_14820"
     CDS             6509..6898
                     /locus_tag="PLH85_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65889.1"
                     /translation="MMKMISRWPVRSVVAWALMWALSVPAGAQLPPSTALLNPVQSAW
                     LQAEHRKVEESFVQKVMGITGASREQVMRAIPARGRITDRLSRIYSALERDLGAPLSD
                     EQKGLIYAAEGDRKQARKDAESAAASR"
     gene            complement(6959..7999)
                     /locus_tag="PLH85_14825"
     CDS             complement(6959..7999)
                     /locus_tag="PLH85_14825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB65890.1"
                     /translation="MIHILKHVESITQQRDRPLLELGVASALFEVVRAREVNLFKVIY
                     GQGMPRVERVTHVGQDGVRYVDTDFVDTIETEVIDARAEMAACVMDRQIVRRHDLDRG
                     VYVHCFPVDVDRRVLGVLELVTPHVLDDEQMGTAEGFLGLYRNYLNILDYSERDTLTG
                     MLNRKTFDDNLGKLLAGLTPSGGNGSSLPARRAVECGRHWLAVIDVDHFKRINDHYGH
                     LYGDEVLLLLARIMQQSFRHQDKLFRFGGEEFVVVLRSSGPGETATALERFRTAVDTH
                     VFPQIGHVTVSIGYTPIRANDTIPDIVGRADEALYFAKDHGRNQTVSHDDLLAKGLIA
                     PRERPQGDVELF"
     gene            8166..9287
                     /locus_tag="PLH85_14830"
     CDS             8166..9287
                     /locus_tag="PLH85_14830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB65891.1"
                     /translation="MNHDTFLNVLFSRVPRADGTRALVVLNVLVFLLTALAAGSLMQF
                     PSDLLIRLGGNFAPLVQKGEAWRLATALFLHGGLLHVSLNMLALHQVGQVVERLYGRS
                     GFLVIYVGAGLLGNLASLWWKQSGVSVGASGAIFGVYGALLAYLTAQRGSVPVQIFAE
                     MRSGTLGFIGYSLFAGFAVPGIDNAAHVGGLLGGLLLGATLARPLVEPDPVRWQSPLA
                     IGGLVVALVLGGVLWIRTPQVVQRFEMNTLFQQNAQQFSVEEQDLLRQQALLMDNLRH
                     RRVTAVQALDTLDNDLIPRWDRQIGVLSDHLAAPEQDWQRVELIHYATARRDALKALA
                     QAISTRQAAWVQQANDLEQQANNILLQMRLRNSLEASRR"
     gene            9362..>11366
                     /gene="dnaE"
                     /locus_tag="PLH85_14835"
     CDS             9362..>11366
                     /gene="dnaE"
                     /locus_tag="PLH85_14835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNB65892.1"
                     /translation="MSESHAPRFVHLRLHTEYSISDGIVQVDAAIARAAADGMPALGI
                     SDLANLFGMVKFYKGARGKGIKPVVGADCWITHDADRDKPARVLLICRNRAGYGQLCE
                     LLTRAFMENKYRGRAEIRREWLTADTASDLLCLSGARDGDIGQALAAGNLALAERLAA
                     DWAARFPDAFYIELQRAGHPGTEAYIRDAVGLASRLALPVVATHPVQFTRPEDFKAHE
                     ARVCIAQGTVLADKRRARDFTEAQYFKTQDEMCALFADLPEALANSVEIARRCSLTVQ
                     LGKNFLPLFPTPDGMSLDDFLILEAKKGLEERLVQLYPHPDERAAQRQRYEDRLKFET
                     DTIIQMGFPGYFLIVADFIQWAKNNGVPVGPGRGSGAGSLVAYSLKITDLDPLAYALL
                     FERFLNPERVSMPDFDIDFCQDNRYRVIEYVRERYGKDAVSQIATFGTMASKAVVRDV
                     GRVLDLPYGLCDRLSKLIPVVQNKPLSLADARAQEPQINEMMVDGNDGESIQELWGLA
                     EPLEGLTRGVGMHAGGVLIAPGKLTDFCPLYIADGEDATPVSQFDKDDVEAVGLVKFD
                     FLGLRNLTIIELALEYVERLTGARPDLMSLGFEDPAAYQILKDANTTAIFQVESEGMK
                     KLLKKLAPDRFEDIIAVLALYRPGPLGSGMVDDFILRKKGQQAI"
BASE COUNT         1849 a         3770 c         3804 g         1943 t
ORIGIN      
        1 gcggcatgtg gatcggcacc ttccacggcc tgtgcaaccg cttcctgcgc gcgcatcacc
       61 gggatgcggg cctgccccag ctgttccaga tcctcgactc gggcgaccag ctggccgcca
      121 tcaagcgcct gctcaagacg ctcaatgttg acgacgagaa gtttccgccc cgcgacctgc
      181 agcacttcat caacggccag aaggaggcgg gcatccgacc gccagccgtg gaggcctggg
      241 acgacttcac acgccgcaag gtggagcttt acgcggagta tgaggctcag tgccagcgcg
      301 agtcagttgt ggatttccct gagttgttgc tgcgctgcta cgaggtgctg gagcgcaacg
      361 agcccctgcg caggcattac caggcgcgct tccgtcacat cctggtggat gagttccagg
      421 acaccaaccg ccttcagtac cgctggttga gcctgctcgc cgacggcgga cgggagggcg
      481 gcgcgcgggt cttctgcgtg ggggacgatg accagtccat ctaccgcttc cgcggggccg
      541 agatcggcaa catgcgggat ttcgagcggg cctttcacgt gaccgacgtg atccgcctgg
      601 aacagaacta ccgttcccac ggcaacatcc tggatgccgc gaatgcgctg atcgcccaca
      661 accgggagcg tctgggcaag catctatgga ccgaccgggg cgccggcgag ccgatccgcg
      721 tctacgaggc cttcaccgat accgacgagg cgcgctggat cgtcgaggag atccaatccc
      781 tggtgcggga tggcgtgagc cgctccagcg tcgccctgct gtaccgttcc aacgcccagt
      841 cgcgggtgct ggagcatcag cttttctcca acggcgtcgc ctaccgggtg tacggcggcc
      901 tgcgcttctt cgagcgccag gagatcaagc acgccctggc ctacctgcgc ctgctggcca
      961 atccggatga cgacacggcc ttcctgcggg tggtcaactt cccgacccgc ggcatcggcg
     1021 cccgctcgct ggaggccctg caggacgcgg cgcggacctg gaacacgccc ctctaccaga
     1081 ccgtcccgca cctgtccggc aagccgggca cggcgctggc ccagttcgtc accctgatcg
     1141 aacgcctgcg ccacgagacg cgggatctgc cgctgcacga gagggtggat cacgtgctgg
     1201 aacgctccac gctgcgggac cattacaagg gtgagaagga aggccaggag cgcctcgaga
     1261 acctggacga actgatcaac gcggcggcca acttcagcgc cgaattccag gtgaacggcg
     1321 cctccgaagg tgcgcccgac acggccaccg tgctcgccga attccttgcc cacgcctcgc
     1381 tggaggccgg cgaccaccag gccgacgcgg gcagcgaggc ggtgcagctg atgaccgtcc
     1441 acgcggccaa gggtctggag ttcgatgtgg tcttcctgtc cgggctggag gaggggctgt
     1501 ttccccacga gaacagcatc ctggaaagcg aagggctgga ggaggagcgg cgcctgatgt
     1561 acgtggccgt gacccgcgcc cgccagcgtc tgtacttgag cttcacccag agccgcatgc
     1621 tccacggcca ggtgcgctac aacctgcgtt cgcgctttct ggaagagatt ccggacggtc
     1681 tgaccaaatg gctgacgccg cccaataccc gtcacggcgg ctttgcggcg gaatctccgg
     1741 cgcgttactt cacgccgtcg tcggcgcgtc cggcggcggc cgtgatgccg gtctcgccac
     1801 cgctcaagtc gcgggatctg ggcggatttg cgatcggcca gaatgtccgc catgcgcgct
     1861 tcggcgacgg ggtgatcgtg gcggccgagg gcagcggggc cgatgcgcgg gtgcaggtca
     1921 agtttggcgc tgccggcagc aaatggctga tgctgtcgat ggcaaaactg acggccgcct
     1981 gagcgacgcc gagccgggtg ccgccgccgg ccccggtcgc cacgcccgac gggccggcgc
     2041 tcagcccttc agctcgccgt gcagggcgat gaattcctgg gccagcttgt ggcgcggctc
     2101 caggtggatc atgggcttgg cctgttcatg gctctcgcgg atcttgatgg agctggacag
     2161 gtaggtcttg aagatgggca gaccttcggc aatcagttcc tgcaccagtt tctggggcag
     2221 gctggcgcgg ggctggtact ggttcaccac gatgccctcc acttccagat cgcggttgtg
     2281 gtcggccttg atctcctgca cgttctccag cagcgcgtag agcgcccggc gggagaagtc
     2341 gtcgcagtcg aagggaatca ggcagcggcg cgtggcgatg agggccgagc gggtgtagaa
     2401 gttgagcgcc ggcggggtgt cgatatagac ggcgtcgaac cggtcggcca gttcgtccag
     2461 ggcatcgcgc agcttgtaga tcttgtagcg ggattccagc ttgctctgca gctcttcgag
     2521 gctggggtgg gagggcagca gcgagagacc gggaaagggc gtgggctgaa cgaattcggc
     2581 tgtctggcgg ggattgaact tgaaagcgag ggtctgctcg aagagccccg cgagggtgtc
     2641 gtcggcgttt tcgagggcgt gccccagcag gtaatgggtc gaattgccct ggggatccag
     2701 gtccaccacc agcgtgcgca ggccctggtg ggcgctgatc gccgccaggt tgcaggtgat
     2761 ggtggatttg cccactccgc ccttctggtt gaataccacg cgccgcatcg tgcctccccg
     2821 gatcattgtt gattgacatg cctgtcgttg cgcgcgattg tgccatgcaa tcaccccggc
     2881 cgtgccggag ggtgcgcaaa tgcgcccgct cgggtacact ccggccaaac aacatgtagt
     2941 cttcgcgacc tcctcccggt ggcgaaactc cggtgtcgcg ggcatgcccg tggcactgtc
     3001 aacgagagca ccggccccga gtcggggtac ccgccgccgc acacatttca gatttccggc
     3061 tgacaagacc ggcacgccgc cgcatccgct ttcccgtgcg ggtgccttcc ggcagccggc
     3121 agagccgttt tcacacgaga gagaacacat ggacaaggat ttcatcgccg ccgccctcga
     3181 gtaccaccgc agcccgaccc ggggcaagat tgccgtggtg cccaccaagg gcctgaccaa
     3241 ccagcgtgat ctggcgctgg cctattcgcc cggcgtggcc gcggcctgcg acgccatcgt
     3301 ggccgatccg gccgatgcgc aggaatacac ctcccgcggc aacctggtgg ccgtggtcac
     3361 caacggcact gcggtgctgg gcctgggcaa catcggcccg ctggccgcca agccggtgat
     3421 ggaaggcaag ggctgcctgt tcaagaagtt tgccaacatc gacgtgttcg acatcgaact
     3481 ggccgagaac gacccggaca agctcatcga catcatcgcc gcgctggagc cgaccctcgg
     3541 cggcatcaac ctggaagaca tcaaggcgcc cgagtgcttc tacatcgaga agaagctgcg
     3601 cgagcgcatg aacatcccgg tcttccacga cgaccagcac ggcacggcca tcatttctgc
     3661 ggcggccatg ctcaacggcc tgaaggtggt gggcaaggac attggcgcgg tcaaggtggt
     3721 gtgttccggc gccggcgcgg cggccatcgc ctgtctggac ctgtggtgca gcctgggcat
     3781 ccgtcgcgag aacgtcttcg tgtgcgactc caagggcgtc atctacgtgg gccgcgagga
     3841 gaacatggag cccaccaagg cccgctacgc ccagaacacg gccttccgca ccctggccga
     3901 tgcgatggtc ggcgccgacg tgttcctcgg cctgtccacc gcgggcgtgc tcaagcccga
     3961 gatggtcaag accatggccg acaagccgct gatcttcgcg ctggccaacc cgacgcccga
     4021 gatcatgccc gagctggcca aggaggtgcg cccggacgcc atcatcgcca ccggccgttc
     4081 ggactacccg aaccaggtga ataacgtcct gtgcttcccc ttcatcttcc gcggtgccct
     4141 ggatgttgca gccaccaaga tcaccgacga gatgaagctc gcctgcgtca aggccatcgc
     4201 cgagctggcc gaggcggagc agtccgacgt ggtggcccag gcctacggcg gcgcggacct
     4261 gagcttcggg ccgaactacc tgattcccaa gcctttcgac ccccgtctga tcgtcaagat
     4321 cgccccggcg gtggcccagg ccgccatcga atccggcgtg gcgacccgtg ccatcgacat
     4381 ggacgcctat gtccagagcc tgaccgagtt cgtctaccag tccggcatca tcatgaagcc
     4441 ggtgttctcc aaggccaagg ggatcccggc cgacaagaag cgtgtgctgt acaccgaagg
     4501 cgaaagcgag cgcgtcctgc gcgccgtgcg cgccgtggtg gacgagaatc tggcgcggcc
     4561 catcctcatc ggccggccgg cagtcatcga catgcgtatc cagaagttcg gcctcaacct
     4621 tcaggccggg cgtgatttcg acgtggtgaa cgtggagtcc gacagccgct accgggagct
     4681 gtggcacgag tactaccgcc tcatggggcg cgacggcgtc accccggatt ccgcccgcga
     4741 agccctgcgc agcgacacca cgctgatcgg ctgcatgctg ctgcgccgcg gggatgccga
     4801 cgcgctggtg tgcggtacca ccggtgccta cgaccaccat ttcaaccacg tcaggaatct
     4861 ggtcggcctg aagaaggggg ccagcctgtt tgcagccatg aacatgctgc tgctgcccaa
     4921 gcgcgtgctg gccattgccg acacctacgt gaatgaaatg cccggtgccg aggagattgc
     4981 cgagatcacg gtgatggcgg ccgaagagct caagcgcttc ggcatcgaac ccaaggcggc
     5041 cctcctgtcc cactccaact tcggcagcgc gccgtccgcg tcgtcccgca agatgcgtgc
     5101 agcccgtgat ctcctgcgcg tgcaggcgcc cgacctgcaa tgcgacggtg agatgcacgg
     5161 cgactcggcc ctgtcggccg aaattcgtga ccgcctgcat ccggactcga ccctcaccgg
     5221 cgaagccaac ctgctggtga tgcccaacct ggacgcggcg aacatcgcct tcaacctcat
     5281 caaggtggca aacggggatg gcgtgtccgt gggtcccatc ctgctcggcg cggccctgcc
     5341 ggtgcacatc ctcaccccgt cggcgtcggt gcgtcgtctg gtgaacatca ccgccctggc
     5401 ggtggtggat gcagccgaac agcgcgtgga aaaggtctga gccaccgtcg gcgcccgccc
     5461 gccgacgggc ggtgcgggcg ccggccgggt gcgatccggt acaatcgggc tttccgtccg
     5521 accccgggga gtgctccatg agtcaccaga tccgcttcag ccgcaccggc ggcccggaag
     5581 tccttgagtg gtgcgagtcc gagccgctgc cgcccggtga aggcgaggcg cgggtgcgcc
     5641 accatgccgt cggactcaat tacatcgacg tctaccatcg ttcgggcctg tatcccgtgc
     5701 cgctgccgtc cggcctcggc ctggaggccg ccggcgaggt ggaggcggtg gggccgggcg
     5761 tcaccgggct gaaaccgggc gaccgggtgg cctatgccgg cggtcccctc ggggcctacg
     5821 ccgaggtgcg caatctgccg gccgaccgtc tcgtgatcct ccccgatgcg ctcagctacg
     5881 agcagggcgc cgcgatgatg ctgcagggac tgaccgccca gtatctgctg cggcgcacct
     5941 accgggtgca gtcgggcgac acaatcctca tccatgccgc agcgggcggc gtcgggctga
     6001 tcgtctgcca gtgggcgaaa gccctgggcg cgacggtcat cggcacggtg ggctccgatg
     6061 acaaggcggc cctggcgcgg gcccatggct gcgatcacgc catcgtctat acccgcgaga
     6121 aggtgtcgga gcgggtgcgc gcgctgacgg gggggcaggg cgtgcaggtg gtgtatgact
     6181 ccatcgggcg cgacaccttc atggattccc tggcctgcct cgcgcccctg gggatgatgg
     6241 tgtcctttgg caatgcgtcc ggtccggtgg atgcctttga tccgggcgtg ctggcccgca
     6301 tgggctccct gttcctgacc cgcccgagcc tgttccatta cgcggcgcgc cgtgagcagc
     6361 tcgtcgccat ggcggacgag ctcttcgccg tcgtcacctc cggtcaggtc cgcctgtcca
     6421 tcggccagcg ctacgcgctg caggatgtcg ccgcggcgca ccgggatctg gaggcacgac
     6481 ggaccaccgg atccacgata ctgattccat gatgaagatg atttcccgat ggcccgtccg
     6541 gagtgtcgtg gcgtgggccc tgatgtgggc cctgtcggtg ccggccggcg cccagctgcc
     6601 gccttcgacg gcgttgctca acccggtcca gtcggcctgg ctgcaggcgg agcaccgcaa
     6661 ggtggaggag tccttcgtgc agaaggtgat gggcatcacg ggggcgagcc gcgaacaggt
     6721 gatgcgggcg attcctgcca gagggcggat cacggaccgc ctgagccgca tctacagtgc
     6781 cctcgagcgc gatctcggcg cccccctcag cgacgagcag aaagggctca tctatgcggc
     6841 ggaaggggat cgcaagcagg cgcggaagga tgcggagagt gccgcggcca gccgctgaac
     6901 ggctggattg acactccgcc tgagccggtc ggatgcgccc cgcggggcgc cggtcggttc
     6961 agaacagttc cacgtcgccc tgggggcgct cgcggggggc gatcagaccc ttcgccagca
     7021 ggtcgtcgtg gctgacggtc tggttgcggc cgtggtcctt ggcaaaatac agggcttcgt
     7081 cggcccgtcc gacgatgtcc ggaatggtgt cgtttgcgcg gatcggcgtg tagccgatgc
     7141 tcaccgtgac gtgaccgatc tgcggaaaga cgtgggtgtc cactgcggtc cggaaacgct
     7201 cgagggcagt ggccgtctcg ccgggccccg acgagcgcag aaccaccacg aactcctcgc
     7261 cgccgaagcg gaacagcttg tcctggtgcc ggaagctctg ttgcatgatg cgcgccagca
     7321 gcaacaggac ctcgtcgccg taaagatgac cgtaatggtc gttgatgcgc ttgaagtgat
     7381 ccacgtcaat gaccgccagc cagtggcgac cacattcgac ggcacggcgg gcgggcaggg
     7441 aactgccgtt tccgccggag ggtgtcaggc cggcgagcag cttccccaga ttgtcgtcga
     7501 aggtcttgcg gttgagcatg cccgtcaggg tgtcccgctc gctgtaatcg aggatgttga
     7561 gatagttgcg gtacagcccg agaaagccct cggcggtgcc catctgctcg tcgtcgagca
     7621 catggggcgt gaccagctcg agcacgccca gcacgcgccg gtccacatcc acggggaaac
     7681 aatgaacata gacgccccga tccagatcgt ggcgccgtac gatctggcgg tccatcacac
     7741 aggctgccat ctcggcccgt gcatcgatga cctcggtctc gatggtatcg acgaaatccg
     7801 tgtccacgta gcggacgccg tcctgaccca cgtgggtgac ccgctccacc cgcggcatgc
     7861 cctgcccgta gatcaccttg aacaggttga cctcgcgcgc ccgcaccacc tcgaagaggg
     7921 cagaggcgac gcccagttca aggagcgggc ggtcccgctg ctgggtgatc gattcgacgt
     7981 gtttgaggat gtggatcatg ggcgcggtgg acgttcggga tcgtatctta gcgcggctcg
     8041 tcaattgcca ttcgtggtgt gaaacacttt cgttcctcgc cgacgatccg ccgcccgtcc
     8101 gctggcggtc gagctcaagc gaactgcatg cgtataattt tcaggttttc ctgcggcggc
     8161 tttccttgaa tcacgacacc ttcctgaatg tgctgttctc ccgcgtgccg cgggccgatg
     8221 gaacgcgggc gctggtggtg ctcaacgtcc tcgtcttcct gctgacggcc cttgcggcgg
     8281 gcagtctgat gcagtttccg tccgacctgc tgatccgcct gggaggcaat ttcgcgccgc
     8341 tggtccagaa gggcgaagct tggcgcctgg cgaccgccct gttcctgcat ggcggcttgc
     8401 tgcacgtgag cctcaacatg cttgcgctgc accaggtggg ccaggtcgtt gaacgccttt
     8461 acggacgttc gggtttcctg gtcatctatg tcggagccgg tctgttgggc aatctcgcca
     8521 gcctgtggtg gaagcagtcg ggggtcagcg tcggggcgtc cggggcaatc ttcggggttt
     8581 acggcgcgct gctggcctac ttgacggcgc agcgggggtc ggtgccggtg cagatctttg
     8641 ctgagatgcg cagcggcacg ctgggcttca tcggctattc gctgtttgcc gggttcgcgg
     8701 tgccgggcat cgacaatgcg gcgcacgtgg gcggtctgct gggcggactg ctgcttggcg
     8761 ccaccctggc acggccgttg gtggagcccg atcccgtccg ctggcagtcg ccccttgcca
     8821 tcggtggcct ggtcgtggcg ctggtcctgg gcggcgtgct gtggatccgc acgccccagg
     8881 tcgtgcagcg tttcgagatg aacaccctgt tccagcagaa tgcccagcag ttctcggtgg
     8941 aggagcagga cctcctgcgc caacaggcct tgctgatgga caatcttcgc catcgtcggg
     9001 tcactgccgt acaggcactc gataccctgg acaatgacct gattccccgt tgggaccgtc
     9061 agatcggtgt cctgtcggat caccttgcag cgcccgagca ggactggcag cgcgtcgagc
     9121 tgatccacta cgccacggcc cggcgcgatg ccctcaaagc cctggcccag gccatctcca
     9181 cgcgccaggc ggcgtgggta cagcaggcca acgacctgga gcagcaggcc aacaacatcc
     9241 tcctgcagat gcgcctgcgc aattcgctgg aggcgtcgcg gcgctgaaca cggcggcggc
     9301 gttgcgggga ccggtcgtca cggcgtttcc ggcgagccgc ggcggcgggt aaaatcgcgg
     9361 gatgtctgaa tcccacgccc cccgtttcgt ccatctgcgt ctccatacgg aatattcgat
     9421 ctccgacggg atcgtgcagg tggatgccgc catcgcccgc gccgctgccg acggcatgcc
     9481 ggcgctgggt atctccgatc tggccaacct gttcgggatg gtcaagttct acaagggcgc
     9541 ccgcggcaag ggcatcaagc ccgtggtggg cgccgattgc tggatcaccc acgacgccga
     9601 ccgggacaag cccgcccgcg tgctgctgat ctgccgcaat cgcgccggct acggccagct
     9661 ctgcgagctg ctgacccgtg ccttcatgga gaacaagtac cgggggcgcg ccgagatccg
     9721 ccgcgaatgg ctcaccgcgg atacggcgag cgacctgctg tgcctgtccg gtgccaggga
     9781 cggtgacatc ggtcaggccc tggccgccgg caatcttgcg ctcgccgagc gcttggcggc
     9841 agactgggcg gcgcgtttcc cggacgcctt ctacatcgag ctgcagcgtg ccggccatcc
     9901 tggcaccgag gcttacatcc gcgacgcggt gggcctcgcg tcgcgtctgg cgctgccggt
     9961 ggtggcgacc catccggtgc agttcacccg tcccgaggac ttcaaggccc acgaggcccg
    10021 ggtgtgcatc gcccagggca cggtgctggc ggacaagcgg cgggcgcggg acttcaccga
    10081 agcgcagtac ttcaagaccc aggacgagat gtgcgccctg ttcgccgatc tgcccgaggc
    10141 gctggccaac agcgtggaga tcgcccggcg ctgctccctc accgtgcagc tcggcaagaa
    10201 cttcctgccc ctcttcccga cgccggacgg catgagcctg gatgacttcc tcatcctgga
    10261 ggcgaagaag ggcctggagg aacgcctcgt ccagctctac ccgcacccgg acgagcgggc
    10321 ggcccagcgt cagcgctatg aggaccgcct caagttcgag accgacacca tcatccagat
    10381 gggcttcccc ggctacttcc tgatcgtggc ggacttcatc cagtgggcca agaacaacgg
    10441 cgtgccggtg gggcccggac ggggatccgg cgccggctcg ctggtggcct actccctcaa
    10501 gatcaccgac ctggatccgc tggcctacgc cttgctgttc gagcgcttcc tcaacccgga
    10561 gcgggtgtcc atgcccgact tcgacatcga cttctgccag gacaaccgct accgggtcat
    10621 cgagtacgtg cgcgagcgct acggcaagga cgcggtgagc cagatcgcca ccttcggcac
    10681 catggcctcc aaggccgtgg tgcgcgacgt ggggcgcgtg ctggatctgc cctacggcct
    10741 gtgcgaccgc ctctccaagc tgatcccggt ggtgcagaac aagcccctgt ccctcgccga
    10801 cgcccgcgcg caggagccgc agatcaacga gatgatggtc gacggcaacg acggcgaatc
    10861 catccaggag ctgtggggcc tggccgagcc gctggagggc ctcacccgcg gtgtcgggat
    10921 gcacgccggc ggggtgctga tcgcgccggg caagctcacc gacttctgcc cgctctacat
    10981 cgccgacggc gaggacgcca ctcccgtgtc ccagttcgac aaggacgacg tggaggcggt
    11041 gggcctggtc aagttcgact tcctcggcct gcgcaacctc accatcatcg agctggcgct
    11101 ggagtacgtg gagcgcctga ccggcgcccg cccggacctg atgagcctgg gcttcgaaga
    11161 cccggccgcc taccagatcc tcaaggacgc caacaccacg gcgatcttcc aggtggaatc
    11221 ggaaggcatg aagaagctcc tcaagaagct cgcgccagac cgtttcgagg acatcatcgc
    11281 cgtgctcgcc ctgtaccgcc ccggcccgct gggctcgggc atggtggacg acttcatcct
    11341 gcgcaagaag ggccagcagg ccatcg
//
LOCUS       DAOQMM010000115        11348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2627_length_11348_cov_49.750996, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000115 DAOQMM010000000
VERSION     DAOQMM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_11348_cov_49.750996"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLH85_14840"
     CDS             <1..300
                     /locus_tag="PLH85_14840"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNB65893.1"
                     /translation="YHLLRGIYRGAHLHEEAVEERQQARLHSLTLEAGAYAIGRTLAE
                     LHLRELGVEVSALRRKRIRVVDPTPETRLELDDVLVLLGTPAQLANTETRLLKGL"
     gene            complement(301..972)
                     /locus_tag="PLH85_14845"
     CDS             complement(301..972)
                     /locus_tag="PLH85_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNB65894.1"
                     /translation="MAHSQLTSRQRGFTLVEIAVVLVIIGLLLGGVLKGQELINSAKV
                     KSLINDFRNVATFAYAYQDRFRALPGDDAGAASHLSGGVKASTGGTAGNGRIEGNWNS
                     TTATDESVLFWQHVRLANLASGDGRNPGLEGLVILDWLPRNAAGGRTGITGVAPITGW
                     AGNFFVCQDNIPGSLARQVDIAMDDGQPAAGSVRLLANGSASGSAVALADLADGTPYT
                     VCAAL"
     gene            complement(1111..2418)
                     /locus_tag="PLH85_14850"
     CDS             complement(1111..2418)
                     /locus_tag="PLH85_14850"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB65895.1"
                     /translation="MSVSAVHRLLARLAGVPRPSSDQEDAWLERTFFVLTGLLTAGAS
                     ALSELFRQVAGAGTSPGITVFLVLGIGLNLAASRRRADFRPCAHVQASLILGAPFLAH
                     LLQTDLIGDAGRLPWAMLAPLGTLLCLDARASRRWFVTLLGVTAGTVAFDQSRLPRHP
                     GMPDGVTLLAVAVDLTLLAGMLYFLLHFAVTQKVQTRQRLRAAHRRLRDEQQHAESLL
                     LNILPAPIAARLKLSRGTLADGHPEVIVMFADIVDYTRIASGMQPTEVFEMLNRVFCR
                     FDELAEHHGLERIKTIGDAYMVAGGLHAHPNEPHAAMAAMALDMQAAVRDHAVTGGIT
                     LELRIGVASGPVVAGVVGRRKFIYDLWGDTVNVAHRLCTEAQPSQIQCDSRLYERVRG
                     RFVFAAPVLLFLKGKGYVPVYRLRSPRVLAVHREPPHPSHRAA"
     gene            complement(2563..3276)
                     /locus_tag="PLH85_14855"
     CDS             complement(2563..3276)
                     /locus_tag="PLH85_14855"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNB65896.1"
                     /translation="MTDRPSRSSARGFTLVEMAVVLLILAILAGGLTAGLSAQVARRN
                     EAETDAALDEARDALLGYALRRGTLPCPARSGSDGAEDRESLTTCRGNRLNGLLPWAE
                     LGISGLDAWGRRLHYAVAPDYVKAITLDSAGKLDIRTRNSAGTELTLTTAIGAAPALV
                     LSHGPNGLGGIDADGNALAAPPAGSDEARNATAGSTLFYARSLSENSDAAGGAFDDRV
                     VWVSPYLIANRLASAGRLP"
     gene            complement(3309..4472)
                     /locus_tag="PLH85_14860"
     CDS             complement(3309..4472)
                     /locus_tag="PLH85_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65897.1"
                     /translation="MVALALVVLLALGATGWFLARRDSNAARHLAREQRTADALRTAR
                     DALIGFAATYRNSLHPDADFGYLPCPDLDGDGSSETCGVQGDTRVGRLPTLTLGLPDL
                     RDGDGECLWYAVAGSFKNNPKADILNPDSTGRFQLLDAAGAPRSLPGDQAGLAAAVIF
                     APGPPLPTQQRQSSTGRCGGDLSASAVGAYLESLQPLSGPGVLTVDPRGSTGNDRIVW
                     ITGDDIMRALKARASFATHLNTVLDATADCLRRAGIPAPARIESTANGLQLGRVPAFS
                     GNAGACTSAGLRDAVENWGELMRYARCTDGTACLSSATGPCRGVLLFGGERLPAGQPR
                     VTDADKALAAHYLESTTLAALSARQLGGLPSRILIPFARRSEPASADVAVCLP"
     gene            complement(4508..4885)
                     /locus_tag="PLH85_14865"
     CDS             complement(4508..4885)
                     /locus_tag="PLH85_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65898.1"
                     /translation="MRPFACCPSPAPSVLTGPAVLVAVLLLTATPADAEPSGRLFHTP
                     EERQRLDRLLDNAASGRAQPERLDGIITRGDGRTTLILDGHPRPPAADLRPLDARSAR
                     ILANDGQTHRLRVGERLPPLSVP"
     gene            complement(4947..6749)
                     /locus_tag="PLH85_14870"
     CDS             complement(4947..6749)
                     /locus_tag="PLH85_14870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB65899.1"
                     /translation="MMENRPPAPRRSKMIDTSQPSDLARETLRQLALRKIAPTPDNYR
                     ALYHEIAGTQAEDGFPDRMLRQIGAALPRHSRDAVRFAQAFEDATARQDWQAVKTSLT
                     EAFTALDVPELNWPALLRELITEWDRRQAGLTQARKREMLDRVLSASGAHPERLHERL
                     QALVRAWARNSDDDSTDSTAATESGEVRAQAERMTSDTPVPADSSGVELLQRLLTGLE
                     AVVVHLDPEAAHELQAIAAHLQPGAPSAPVGELRTRLDRLLSHLNWVAEDHAALRDAL
                     LRVLKLIVDNISKLVMDDQWLRGQMSLLDEVFSHPLDIRTLGELESRLRDVIARQAAL
                     KHELSDAHAKLKDMLARFVDRLGEFSDSTGDYHDKIEQCAGRISTARDISELTDVIAE
                     VMKETRSIQESTLRSRTELEELRGRVENANVEINRLQNELEQTSELVRHDPLTGTLNR
                     KGLDDAFARETALARRRNTRLCVALLDVDNFKHINDTHGHQTGDEALQHLAAVIRENL
                     RPQDSVARYGGEEFVILLPDTDVDLATATLVRLQRALTKRYFLARQQKLLITFSAGVA
                     ELQADEDTESALDRADKAMYIAKRSGKNRVISAT"
     gene            complement(6878..7519)
                     /locus_tag="PLH85_14875"
     CDS             complement(6878..7519)
                     /locus_tag="PLH85_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB65900.1"
                     /translation="MTKTIRVLIADDHAIVRQGLRQILSDTPDLTVAGEAENGVQAVQ
                     MVRNGEWDVVLMDVSMPDRNGIDALKLIKKEFPRLPVLILSMYPEEQYAIRALKSGAA
                     GYLTKQSAPELLVTAIRQVASGKKYVSPSLAEELVNAIGEDTERPAHEKLSDREYQTL
                     CMIASGKTPTEIAEALNLSVKTVSVYRARLLEKMHLRNNAELTHYGLKHGLAE"
     gene            complement(7516..9075)
                     /locus_tag="PLH85_14880"
     CDS             complement(7516..9075)
                     /locus_tag="PLH85_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB65901.1"
                     /translation="MPDAPQTDALTEGGDAPGQPIRLLFIEDSENDMLLLLHHFRSAG
                     YAPHFRRVASEAGLLDALADTTWDVILCDHKLPGFSAQGALRLIQERALDLPFIIVSG
                     VIQEDEAIAAMRAGAHDYLSKSKLDRLIPAVERELREVCNRREKRLAEQTLRESEARQ
                     RALTDNIPGVVFRMGFTSDGMLGFQYLSEAASMLFGTSADELIGGSAGWMTWLLDDDL
                     PGFIEAADLSAAHLSTLNWEGRIQLSSGEQKWINLRSSPRLSEAGEVVWEGVMWNITH
                     SKRVESDLRESRSQLAALSNHLQRIKEDERERIARDVHDVLGGTLVAMKFEMSLLESK
                     LESHPAQARERARNVGRMVDDAIATVGRVTRELRPGILKDFGLPAAIESQGEDFAQRT
                     GIQVKILCNDHDLAPDYDTALALFRVFQEALTNVSKHARASQVGVRLLQEGDEVLLEI
                     SDDGVGIDAADLQKPRSFGLRGIRERMSSLGGRLDVDPVLPHGTRLTLRAPLYDPTAT
                     ERPPQLRSHAR"
     gene            9219..9291
                     /locus_tag="PLH85_14885"
     tRNA            9219..9291
                     /locus_tag="PLH85_14885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9252..9254,aa:Thr,seq:tgt)
     gene            9607..10059
                     /locus_tag="PLH85_14890"
     CDS             9607..10059
                     /locus_tag="PLH85_14890"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNB65902.1"
                     /translation="MCKSARSVLFRLFLWCALVVPVSAWANDHGGGGGGSDLVNMETI
                     TTNLAPEPGGNVRFIQLTIALRLHDPSGAAAISAYMPQIRNDILGLLASRTGAELLIT
                     KAREDLSDDIQDAVNAVVGTAAYTDKKGHAHRAEGPVKRALFTTFIVQ"
     gene            complement(10406..11074)
                     /locus_tag="PLH85_14895"
     CDS             complement(10406..11074)
                     /locus_tag="PLH85_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HNB65903.1"
                     /translation="MPYILRSVLVLTALSGLALPAQALSPALAPNASVTVDRAGVFDY
                     YVLSLSWSPTFCLTHADNSQCSKGYGFVLHGLWPQYARGGFPQDCYSPERLTPEAVRF
                     GRTIFPAENLIDHEWRKHGTCSGLSALAYFRAADRARTQINVPAPLESPATTHSDTAA
                     AIARSFVAANPGIPSGSVTVVCSGPQLSEVRLCLNTSLAPVACGKGVTHACRGGPIRI
                     PSVR"
BASE COUNT         1663 a         3369 c         4398 g         1918 t
ORIGIN      
        1 tatcacctgc tgcgcggcat ctaccggggt gcccacctgc acgaagaggc cgtggaggaa
       61 cgccagcagg cgcgcctgca ttccctgacc ctggaggccg gcgcctatgc catcgggcga
      121 accctcgcgg agctgcatct gcgcgagttg ggggtggagg tgagcgccct gcggcgcaaa
      181 cgcatccgcg tggtggaccc gacgccggaa acccgcctgg aactggacga tgtactggtg
      241 ctgctgggca cgccggccca gttggccaac accgagacgc gcctgctgaa gggcctctga
      301 tcagagggct gcgcagacgg tgtaaggggt gccgtcggcc agatcggcga gggccacggc
      361 gctgcccgag gcgctgccat tggcgagcag acggaccgat ccggcggcgg gctggccgtc
      421 gtccatggcg atatccactt gccgcgcgag gctgcccggg atgttgtcct gacacacgaa
      481 gaagttgccc gcccagccgg tgattggcgc gacgccggtg atgccggtgc gtccgccggc
      541 ggcgttgcgg ggcagccagt cgaggatcac cagtccttcg aggcccggat tgcggccgtc
      601 gccgctggcc aggttggcga gacgcacatg ctgccagaac agcacggatt cgtcggtggc
      661 ggtggtggag ttccagttgc cttcgatgcg tccgttgccg gcggtgccgc cggtgcttgc
      721 cttgacgccg ccggacaggt ggctggcggc gcccgcatcg tcgccgggca gggcccggaa
      781 gcggtcctgg taggcgtacg cgaaggtggc gacgttgcgg aagtcgttga tgaggctctt
      841 caccttggcg ctgttgataa gttcctgccc cttcagcacg ccgcccagca acaggccgat
      901 gatgaccagc acgacggcga tttcgacgag ggtgaagcct cgctgtctgc tggtgagttg
      961 agaatgcgcc atcttgctcc ccgtttccgt tggtgtatgg acgtgtggat gcatccatgc
     1021 atataaatta tgccagccgg aattctaggg catcccgcgg tcggcgcgga agggggagtg
     1081 tggttgtgcg ggtgccgtct gcgcggaggc tcaggcggcg cggtggctcg gatggggcgg
     1141 ctcgcgatgc acggcgagga cgcgcgggct gcgcagccgg tagacgggca cgtagccctt
     1201 gcctttgagg aagagcagca ccggcgccgc gaacacgaag cggccgcgca cccgctcgta
     1261 gagccggctg tcgcactgga tctggctggg ctgggcttcg gtgcacaggc gatgggccac
     1321 attgacggtg tcgccccaca ggtcgtagat gaacttgcgc cgtcccacca cgccggccac
     1381 gacggggccg gaggcgacgc caatgcgcag ctccagcgtg atgccgccgg tcaccgcgtg
     1441 gtcgcgcacg gcggcctgca tgtcgagggc catggcggcc atggcggcgt ggggttcgtt
     1501 ggggtgggcg tgcagtccgc cggccaccat gtaggcgtcg ccgatggtct tgatgcgctc
     1561 caggccgtga tgttcggcca gttcgtcgaa gcggcagaaa acccggttga gcatctcgaa
     1621 gacctcggtg ggctgcatgc cgctggcgat gcgggtgtag tccacgatgt cggcgaacat
     1681 cacgatgact tcggggtggc cgtcggcgag ggtgccgcgg ctgagcttga ggcgcgccgc
     1741 gatgggtgcc ggcaggatgt tgaggagcag gctttccgca tgctgctgct cgtcgcgcag
     1801 ccgtcggtgg gcggcgcgca ggcgctggcg ggtctggacc ttctgggtga cggcgaagtg
     1861 cagcaggaaa tagagcatcc cggccagcag ggtgagatcg acggcaacgg ccaggagggt
     1921 cacgccatcg ggcattcccg ggtggcgcgg gaggcggctc tggtcgaagg ccaccgtgcc
     1981 ggcggtcacc cccaggaggg tcacgaacca gcggcgggag gcgcgggcat ccaggcacag
     2041 cagggtaccg agcggggcca gcatggccca cggcaggcgg ccggcgtcgc cgatgaggtc
     2101 ggtctgcagc aggtgcgcga ggaacggagc gccgaggatc aggctggcct ggacgtgggc
     2161 acagggccgg aagtcggcgc ggcgccggct cgcggcgagg ttgaggccga tgccgagaac
     2221 aaggaacacg gtgatgcctg gtgacgtgcc cgcaccggcc acctggcgga acagttcgga
     2281 aagtgcgctt gcgccggccg tgagcaggcc cgtgaggacg aagaaggtgc gttcgagcca
     2341 tgcgtcctcc tgatcggaag acggccgggg aacgcccgcc agccgggcca gcaggcgatg
     2401 gaccgcggag acggacatgg cggatcctgc gtgagtcgag tggcggcagt gtccaagggg
     2461 gcgataccgc ggtcaaccct caaagtcgcg atattcgtgc aaatgtgttt cgtgtgctgg
     2521 cgaattttgt gttttattcg gatggaatgc ggtgtgccgg cttcagggca aacggccggc
     2581 gctcgccaga cggttggcga tgaggtaggg cgacacccac accacccggt cgtcgaaggc
     2641 gccaccggca gcatcggaat tttcggacag gctgcgggcg tagaagaggg tgctgccggc
     2701 ggtcgcattg cgggcttcgt cggagcccgc cggcggtgcg gcgagggcgt tgccgtcggc
     2761 gtcgatgccg cccaggccgt tggggccgtg ggacaggacc agggcgggcg cggcgccgat
     2821 ggcggtggtg agggtgagct cggtgccggc gctgttgcgg gtgcgtatgt ccagtttgcc
     2881 ggcgctgtcg agggtgatgg ccttcacgta gtccggcgcg acggcatagt gcaggcgcct
     2941 gccccaggca tcgaggccgg aaatccccag ttcggcccag ggcagcaggc cgttgaggcg
     3001 attgccacgg caggtggtga ggctttcccg atcctcggcg ccgtcgctgc cactcctggc
     3061 ggggcagggc agcgttccac ggcgcagcgc gtaaccgagc agggcgtcgc gggcctcgtc
     3121 cagcgcggca tcggtctcgg cttcgttgcg gcgggccacc tgggccgaca ggccggcggt
     3181 gaggccgccc gccaggatgg ccaggatgag caggacgacg gccatctcca cgagggtgaa
     3241 gccgcgggcg ctggagcggg agggtcggtc ggtcatggtc ggacgggatc gaacgggatt
     3301 gggcgggttc agggcaggca cacggccacg tcggcgctgg ccggctcgct gcggcgcgcg
     3361 aaggggatga ggatgcggct gggcaggccg cccagctgcc gggccgacag ggcggcgagg
     3421 gtggtggatt ccagatagtg ggcggccagc gccttgtcgg cgtcggtcac gcggggctgg
     3481 ccggcgggca ggcgttcgcc gccgaacagg agaacgccgc gacaggggcc cgtggcgctg
     3541 ctcaggcagg ccgtgccgtc ggtacagcgg gcgtagcgca tgagttcgcc ccagttctcg
     3601 acggcgtccc gcaggccggc actggtgcag gcgccggcat tgccactgaa agccggaacg
     3661 cgtccaagct ggaggccgtt ggctgtggat tcaatgcgcg ccggtgccgg tatgccggcc
     3721 cgccgcaggc agtcggcggt ggcgtcgagg acggtgttca agtgggtggc gaagctggcg
     3781 cgggccttga gggcacgcat gatgtcgtcg ccggtgatcc agacgatgcg gtcgttaccg
     3841 gtgctcccgc gcggatcgac ggtcagaacg ccggggccgg agaggggctg cagcgattcc
     3901 aggtaggcac ccacggcgct ggctgagagg tcaccgccgc agcgcccggt gctgctctgg
     3961 cgctgttggg tgggcagggg cgggcccggt gcgaaaatga ccgcggcggc cagtccggcc
     4021 tggtcgccgg gcaggctgcg gggcgcgccg gccgcgtcga ggagctggaa gcgtccggtg
     4081 ctgtccggat tgaggatgtc ggccttcggg ttgttcttga aggagccggc gacggcgtac
     4141 cacaggcatt cgccgtcccc gtcgcgcagg tccggcaggc cgagggtgag ggtgggcagg
     4201 cgaccgacgc gggtgtcgcc ttgcacgcca caggtttcgg acgagccgtc gccgtccagg
     4261 tcggggcagg gcaggtagcc gaagtcggcg tccgggtgga gactgttgcg ataggtggcg
     4321 gcaaagccga tgagggcgtc acgggcggtg cgcagggcgt cggcggtgcg ctgttcgcgg
     4381 gcgaggtggc gagctgcgtt gctgtcccgg cgggcgagga accagccggt ggcaccgagg
     4441 gcgaggagga cgaccagcgc gagggcgacc atgccctgcg aacggtggcg acgtgcggcc
     4501 agacggttca aggcaccgac aggggcggca ggcgctcgcc gacgcgcagg cggtgggtct
     4561 ggccgtcgtt ggcaaggatg cgggcggacc gtgcgtccaa gggccggaga tcggcggcag
     4621 gggggcgcgg atgtccgtcg aggatcaggg tcgtgcgccc gtcgccccgg gtgatgatgc
     4681 cgtccagccg ttcaggctgg gcgcggccgg aggcggcgtt gtcgaggagg cggtcgaggc
     4741 gctggcgttc ctcgggggtg tggaaaaggc gtccggaggg ctcggcatcg gccggtgtgg
     4801 cggtgagcag caggacggcg acgagcacag cgggcccggt caggacgctg ggcgcaggcg
     4861 aggggcagca ggcgaagggg cgcatgccag aaatctagca tattccatgc gcgtgtagcg
     4921 gggtgtgccc gatgccgggc gggggctcag gtggcggaga tgacgcggtt cttgccggac
     4981 cgtttggcga tatacatggc cttgtcggcg cggtcgaggg cgctttcggt gtcttcgtcg
     5041 gcctgcagtt cggcgacgcc agcgctgaag gtgatgagga gcttctgctg gcgggcgagg
     5101 aagtagcgtt tggtgagagc gcgctgcagg cgcacgaggg tggcagtggc gaggtccacg
     5161 tcggtgtcgg gcagcaggat gacgaattcc tcgccgccgt agcgggccac ggaatcctgc
     5221 gggcgcaggt tctcgcggat caccgcggcg aggtgctgga gcgcctcgtc gccggtctgg
     5281 tggccgtggg tgtcgttgat gtgcttgaag ttgtccacgt ccagcagggc cacgcacagg
     5341 cgcgtgttgc ggcgccgggc gagggcggtc tcgcgggcga aggcgtcgtc caggcccttg
     5401 cggttgaggg tgccggtcag cgggtcgtgg cggacgagtt cgctggtctg ctcgagctcg
     5461 ttctgcaggc ggttgatctc gacattggcg ttctcgacgc ggccgcgcag ttcctccagt
     5521 tcggtgcgcg agcgcagggt cgattcctgg atgctgcggg tctccttcat gacttcggcg
     5581 atgacgtcgg tcagttcgga gatgtcgcgg gcggtggaga tgcggccagc gcactgttcg
     5641 atcttgtcgt ggtagtcgcc ggtggagtcg gagaattctc cgaggcggtc cacgaagcgg
     5701 gccagcatgt ccttgagctt ggcgtgggcg tcggacagtt cgtgcttgag ggcggcctgc
     5761 cgggcgatca cgtcgcgcag gcgggattcc agctcgccca gggtgcggat gtccagcggg
     5821 tggctgaaga cttcgtccag caggctcatc tgaccgcgca gccattggtc gtccatcacc
     5881 agcttgctga tgttgtccac gatcagcttg aggacccgca ggagggcgtc gcgcagggcg
     5941 gcgtgatcct cggcgaccca gttcaggtgc gacaggaggc ggtcgaggcg ggtgcgcagt
     6001 tcaccgaccg gggctgacgg ggcgcccggc tgcaggtgcg cggcaatggc ctggagttcg
     6061 tgggccgctt ccgggtcgag atggacgacg acagcttcca ggccggtcag cagccgctgg
     6121 agcagttcga cccccgagct gtctgccggc accggcgtgt cgctggtcat gcgttcggcc
     6181 tgcgcgcgga cctcgccgga ttcggtggca gccgtggagt cggtgctgtc gtcgtcgctg
     6241 ttgcgggccc aggcgcgcac cagcgcttgt aggcgttcgt ggagtcgctc gggatgggca
     6301 ccggaagcgg acaggacgcg gtcgagcatc tcccgcttgc gggcctgggt cagcccggcc
     6361 tgacgccggt cccattcggt gatgagctcg cgcagcagcg ccggccagtt gagttcgggt
     6421 acgtcgaggg cggtgaaggc ctcggtcagc gaggtcttga cggcctgcca gtcctggcgc
     6481 gcggtggcat cctcgaaggc ctgggcgaag cgcacggcgt cgcgggaatg acgtggcagg
     6541 gctgctccga tctgtcgcag catgcggtcg ggaaagccgt cttcggcctg ggtgccggcg
     6601 atttcgtggt agagggcccg atagttgtcc ggcgtgggcg caatcttgcg caacgccagt
     6661 tgccggagcg tctcacgggc gagatcggac ggctggctgg tgtcgatcat cttggagcgg
     6721 cgcggagcgg gggggcggtt ttccatcatc cggcgtgccg gtgactcgcc tgacgagccc
     6781 acgaatgagt gacctgtaag aggggaggtt attgcatccg gatgctataa cggccccggc
     6841 gggcggccct tgagctgttc ttgagtacgc cggcggatta ttccgcgagc ccgtgcttga
     6901 gcccgtagtg ggtcagttcg gcattgttgc gcagatgcat cttttcgagc agccgggcgc
     6961 gatacacgct gacggtcttg accgacaggt tgagcgcctc ggcgatctcg gtgggcgtct
     7021 tgccggacgc gatcatgcac agggtctggt attcccggtc ggagagcttt tcgtgggcgg
     7081 ggcgctcggt gtcctcgccg atcgcgttga ccagctcttc ggcgagggac gggctgacat
     7141 acttcttgcc gctggccacc tggcggatgg cggtaacgag gagttccggc gcgctttgct
     7201 tggtgaggta gccggccgcc ccggatttga gggcgcggat ggcgtactgt tcctccgggt
     7261 acatgctgag gatcagcacc ggcaggcggg ggaattcctt cttgatcagc ttgagggcgt
     7321 cgatgccgtt gcgatcgggc atggagacgt ccatcaggac cacgtcccat tcgccgttgc
     7381 gcaccatctg tacggcctgg acgccgttct cggcctcgcc ggcgacggtg aggtcgggcg
     7441 tgtcggagag gatctggcgc agaccctggc ggacgatggc gtgatcgtcg gcgatcagga
     7501 ctcggatggt tttggtcatc gcgcatgact ccggagttgg ggggggcgct cggtggcggt
     7561 ggggtcgtac aagggggcgc gcagggtcag ccgggtgccg tggggcagga ccggatccac
     7621 atcgaggcgc ccgcccaggc tgctcatccg ttcgcggatc ccgcgcagtc cgaaggagcg
     7681 gggcttctgc aggtcggcgg cgtcgatgcc gacgccgtcg tcgctgatct caaggagcac
     7741 ctcatcgcct tcctggagca ggcgaacgcc gacctgcgac gcgcgggcgt gcttgctgac
     7801 attggtgagg gcttcctgga agacccggaa gagggccagc gcggtgtcgt agtcgggcgc
     7861 caggtcgtgg tcgttgcaga ggatcttgac ctggatgccg gtgcgctggg cgaagtcctc
     7921 gccctgggat tcgatggcgg caggcaggcc gaagtccttc aggatgccgg ggcgcagttc
     7981 gcgggtgacg cggccgacgg tggcgatggc gtcgtccacc atgcggccca cgttccgtgc
     8041 ccgctcccgc gcctgggccg gatgggattc cagcttggat tccagcaggg acatctcgaa
     8101 cttcatggcc accagggttc ccccgagcac gtcatggacg tcccgcgcaa tgcgttcgcg
     8161 ctcgtcttcc ttgatgcgct gcaggtggtt cgacagggcg gcgagctggc tgcgggattc
     8221 cctcagatcg gattcgaccc gtttgctgtg ggtgatgttc cacatgacgc cttcccacac
     8281 cacttcgccg gcctcggaca ggcgcggcga gctgcgcagg ttgatccatt tctgctcgcc
     8341 ggaagagagc tggatgcggc cttcccagtt gagggtggac aggtgcgcgg cggacaggtc
     8401 tgccgcctcg atgaagcccg gcaggtcgtc gtcgaggagc caggtcatcc agcccgcgga
     8461 accaccgatg agttcgtccg ccgaggtgcc gaagagcatg ctggcggctt cgctcaggta
     8521 ctggaagcca agcatgccgt cggacgtgaa gcccatgcgg aagacgacgc cggggatgtt
     8581 gtcggtaagg gcccgctggc gcgcctcact ttcccgcagg gtctgctcgg ccaggcgctt
     8641 ctcgcgacgg ttgcagactt cgcgcagctc ccgctccacc gccggaatca ggcggtcgag
     8701 cttgcttttg ctgaggtagt cgtgggcacc ggcgcgcatg gcggcgatgg cttcatcctc
     8761 ctggatcacg ccggagacga tgatgaaggg cagatcgagg gcgcgttcct ggatcaggcg
     8821 cagggcgccc tgggcactga agcccggcag tttgtggtcg cacaggatca cgtcccaggt
     8881 cgtgtcggcg agggcgtcga gcaggccggc ttcggaagcg acccgacgga aatgcggggc
     8941 gtagcctgcg ctgcggaagt gatgcagcag caggagcatg tcgttctcgg agtcttcgat
     9001 gaacagcagg cggataggct ggccgggcgc gtcaccgcct tccgtaaggg cgtctgtctg
     9061 gggggcgtcg ggcatgacgg aaccgtgtgc tgcgtattgg ggacgctttg tttataatcc
     9121 ggccctcgtt cgggcctgct gaggtggaaa gcatagagca ggcctcggtc gcccgtccat
     9181 gccgccgcac aaggcgggac atgggttctg gagtacctgc cggtgtagct cagttggtag
     9241 agcagcgcac ttgtaatgcg aaggtcgagg gttcgattcc tttcaccggc acccgcttat
     9301 tccgcgctct gaacgccttt cgggcgtctc tcccgttcat ctatcctgca tgatccggcg
     9361 tgcatccggg ggctctgccg ggcgtaccgg ggacgatgag cgccgtccat tccggcacga
     9421 aagttatcag atgtgcagcg cagacgaacc tgtctgctgc ccattctggg tagcccttca
     9481 aggcgcggcg gtcattgtcc gttaatagtc ggagcggaag gtgctggaga cgtgcggccc
     9541 tccgcctcgg tggccggaca tgccggtccc tgcccaatcg acgtctgcag ctggcgagtt
     9601 tcccccatgt gcaagtccgc ccgaagtgtc ctcttccgcc ttttcctgtg gtgcgccctc
     9661 gtcgtgccgg tgtccgcatg ggccaacgat catggaggcg gcgggggcgg atccgatctc
     9721 gtcaacatgg agacgatcac caccaacctg gcgccggagc cggggggcaa cgtgcgcttc
     9781 atccagctca cgattgccct gcgcctgcac gatccgtcag gcgccgccgc gatctccgcc
     9841 tacatgccgc agatccgcaa tgacattctg gggctgctgg cgtcgcgtac gggggccgag
     9901 ctgttgatca ccaaggcgcg cgaggacctg tcagacgata tccaggacgc cgtgaatgcc
     9961 gtggtgggca ccgccgccta caccgacaag aaggggcatg cccatcgggc cgaaggcccg
    10021 gtcaagcgcg cgctgttcac caccttcatc gtccagtgag ggcagcggcc atcgccgcaa
    10081 cgtgttcgtg caaggccgag gagcccggac ggtacccgct tccctggagg cgtccccgga
    10141 ccggcgcgtc cgacgcgtga atgtccggtg ggtccttgaa cacggttcgt gcggagcggg
    10201 ggcggcgatc ttcgtcgatc tggggcaacg ccgggcaggc gacgcgggac gttccgcgtg
    10261 gcgggcttcg acggggggcg cgatcagcgc gcccgccccg gtggcaatca ggcgggcatc
    10321 gcgcagtcgg tcacgtgctc cggcgcggtg gcccctgtga tggacggcgc cacgcgtacg
    10381 ccctcgcgga tcaccgccac aggcgtcaac gcacgctggg gatgcggatc gggccgccgc
    10441 gacaggcatg ggtgacgccc ttgccgcagg cgacgggcgc cagggatgtg ttcagacaga
    10501 gccggacctc cgataactgc ggtccgctgc agacgacggt gacgctgccg gagggaatgc
    10561 ccgggttggc ggcaacaaag gaccgggcga tggcggccgc agtgtcggaa tgggtcgtcg
    10621 cggggctttc gagcggggcg gggacgttga tctgcgtgcg ggcgcggtcc gccgcccgga
    10681 agtatgcgag ggcgctcagg ccgctgcagg tgccgtgctt gcgccattcg tgatcgatca
    10741 ggttctcggc cgggaagatc gtccggccga atcgaacggc ttcgggtgtg aggcgctccg
    10801 gcgagtagca gtcctgcggg aagccgccgc gcgcatattg gggccacagg ccgtgaagca
    10861 cgaagccgta ccccttgctg cactgactgt tgtccgcgtg ggtcaggcag aaggtgggcg
    10921 accaggacag ggagaggacg taatagtcga agacacccgc ccgatcgacg gtgacgcttg
    10981 cgttcggcgc cagggcgggg ctgagggcct gggccggcag ggcgagcccg ctcagggcgg
    11041 tcagcacgag cacgctgcga aggatgtagg gcatggacgt tcctggtctg agggctgggg
    11101 cgaacatggg cacgggggcc gtgtggacgc cgggcgtatg catgggtgag gccgctttcc
    11161 attgaagcag gtcgacgcgg cgatgccaac cgggcgcccg tcgggcgacg gttcacgggg
    11221 acgcaaatgc atgcttgtga tgatgcgccg caaaaatact tgactctctc tttgaggttg
    11281 tctattattc gccttctccg acgcggggtg gagcagtctg gcagctcgtc gggctcataa
    11341 cccgaagg
//
LOCUS       DAOQMM010000116        11345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2630_length_11345_cov_44.334898, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000116 DAOQMM010000000
VERSION     DAOQMM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2630_length_11345_cov_44.334898"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..415)
                     /locus_tag="PLH85_14900"
     CDS             complement(32..415)
                     /locus_tag="PLH85_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65904.1"
                     /translation="MNTTARPLPPLLLGLFRELQANGVPVGVRDYLDGLRALALCTGA
                     DADPALRDAFGRPARDSLRDLALALWARSDEERRLITRWFDVLPEPADALVQALDACL
                     ETLDTPEDTAAAAPDDLGSAWPRGE"
     gene            complement(412..1350)
                     /locus_tag="PLH85_14905"
     CDS             complement(412..1350)
                     /locus_tag="PLH85_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB65905.1"
                     /translation="MSATPYKSFTGTATDFYVAAPATATLVNLAVRLGRPILVEGEAG
                     CGKTRLASAIATELGVGLTVMTVKSTSQAKDLLYRFDALRRLQDAQIPGNTTARHVHP
                     YLSLEPLGHAIQSGTPQVVLIDEVDKADIDFPNDLLDVLDRFEFDLEDLPRDEEDACR
                     AARGFGRHVSAPKDGTRPIVLITSNREKQLPEPFLRRCLYLQLDFPEDQPERLADIVR
                     KNLAPRGESVGDTLLAAAVARFCEIRAQGRRQGVRKLPATSELVDWVRTLHWQGRKED
                     SLDPKALDALDTAVLFKLRADIDAYKAGKDEDGGGW"
     gene            complement(1362..2774)
                     /locus_tag="PLH85_14910"
     CDS             complement(1362..2774)
                     /locus_tag="PLH85_14910"
                     /inference="COORDINATES: protein motif:HMM:NF040412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNB65906.1"
                     /translation="MQTGGTRPAQRPPHPPEPMPRAADLPKIERIFLSATATDCLAYR
                     EAVKAAVEGNLRAAKIFLQEKWALNAETAVKACKDRLLDCDAYVGLFGHRYGHIAPGQ
                     TISITEQEFRWAVEHWPHLTPPIFILRAKPGSEADTFLRAQAEQCWHTLPTPEAVEAD
                     RSAQQRFVAAVTDWSAEEGRVLNFYADLIELRDKAQACINHWNLDLLEAALDGRYQAT
                     GEIPAAELGRLGRKPQFTAFRDSLDTFRERRDQRAVAFLVHGPENHGQPQFMEALAQW
                     TTGKDGMEVFAGQASEPDSAESLICWLCSRLNEPLLGEPTVDALARILAKRLERRTVV
                     VLLHTVGREAQRLALFHQRFWQPLADALAALKPGGRSRFICVVADHAPLPAAPGEFIR
                     PADPDDADTDYRQLLALPALAPLNARQVRSWLADLEDAGIVLDDDRRDAIAEHATTPD
                     GLPPNVYYRLNLEGFWKHAR"
     gene            2852..3739
                     /locus_tag="PLH85_14915"
     CDS             2852..3739
                     /locus_tag="PLH85_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNB65907.1"
                     /translation="MRIGIDLGGTKIEIIALDAAGRTLARRRVATPQGDYAATVQAVA
                     GLVAAVESELGQRGTVGVGTPGALSRVTGRIKNANSTCLNGQPLQDDLQAALGRPLRI
                     ANDANCFALSEAVDGAAAGAETVFGVILGTGVGGGIVVRGQALTGANAIAGEWGHNPL
                     PGGAAPGPACYCGRSGCVETWLSGPGLAADHLRVTGRTCTPEALVAGAAAGDADSIAT
                     LDRYAARLARALATVVNLLDPDVIVLGGGLSNLAMLYEAVPRLWVPHVFSDAIATRLC
                     RAVHGDSSGVRGAAWLWPD"
     gene            3845..4513
                     /locus_tag="PLH85_14920"
     CDS             3845..4513
                     /locus_tag="PLH85_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNB65908.1"
                     /translation="MSTPLAVFAGGLRALPPEGQQTGMFKAPVVGPVAVGVDGLAGDR
                     QADRRVHGGPEKAVHYFPAEHYAHLTTRVPALADALRPGVLGENLSARGLTEDTVCIG
                     DVFAAGSCRLQVSQPRNPCWKINHRLDHDRMSRIIVDDGCTGWYFRVLAPGVLTAGEV
                     LRLVERPAPGMTLARLAAVKGAHRPSPDELLAVAAAPGLAPDVARRLQARGAWLTNSG
                     VPPC"
     gene            4586..5035
                     /locus_tag="PLH85_14925"
     CDS             4586..5035
                     /locus_tag="PLH85_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65909.1"
                     /translation="MNPSLEPPRHRAFCTAEFAGRFFLAQLDVSQRMGNLYALLGMGL
                     TDCALRSHVLRATHDAAYTVDLNELDSRKALLLEASLEGFATILRNFAEEVAAAHVRC
                     LEQALGRVPSGAPSALPPALLPFLQPDFAGFGRAFNEALRERAEGRG"
     gene            5160..6416
                     /locus_tag="PLH85_14930"
     CDS             5160..6416
                     /locus_tag="PLH85_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB65910.1"
                     /translation="MTPVPSPPAPPVAPPSAPFLVSRIFLPFALGYYLSYLLRTVNAV
                     ISPDLTRELGLNAADLGLLTSTYFFAFGLAQIPVGIALDRFGPRRVESALLLITALGA
                     TLFALGEDLPVLAGARGLIGFGVSACLMGGLKGFTLWFPRERLASLTGYIMSGGALGA
                     VTASAPLEAALPFIGWRGAFWAVAVLAAGIAALIFFAMPEKSSGHGGGDLQAALRGVK
                     EVLSARRFWSFSGQAAFFTGGFMALQGLWAVPWLMQVNGYSRAVAADHLFWLNLGMLA
                     GQLSIGAWATRLAARGITPLRLMQGGLFLTMLVEALIIARLGPTQPLWCLLGIVSATG
                     AQMYGVVAGLFPLHLSGRVTTSINLMAFVGAFAVQWGLGGLLDGLRAGGMSAATSLEV
                     AFLILLAAQAASFVPLSRAGRYTPVH"
     gene            6519..7325
                     /locus_tag="PLH85_14935"
     CDS             6519..7325
                     /locus_tag="PLH85_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB65911.1"
                     /translation="MSSRQLYVHRYPAAHPEGRPTLLFVHGAYSDGRLWVEHFIPWFR
                     EAGYTCLAVDLSGHGRSEGRDDLDSLGLDDYVDDVAQVVATLRAPPVLIAHSMGSLVA
                     QRYAQRAPLAGMALLAPVPPTGTGGSAGRLALMLPEFFEELPRAIAGSAGPRTLEVMA
                     RVYFSPEMPARETAHYLPLLQAESERAVLEMVTLPLRGLSCRPRLPVLVMGGSQDAVF
                     PASMLCFTALPWRAQQDVVSGAGHMLMLDPQWEDAATRIAAWLETLPAAT"
     gene            complement(7356..7739)
                     /locus_tag="PLH85_14940"
     CDS             complement(7356..7739)
                     /locus_tag="PLH85_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB65912.1"
                     /translation="MPTPIAPPQPALIHFADPAPAASEDRPAPERALGPPPRRLTAER
                     YSAEDGALSIGEWTCEPGAWRIAFHAGRHEFFQVLSGRLRLIDDAGHARDFGPGDACV
                     IPAGFKGVFEVLETVTKRYVMVDRA"
     gene            complement(7762..8328)
                     /locus_tag="PLH85_14945"
     CDS             complement(7762..8328)
                     /locus_tag="PLH85_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65913.1"
                     /translation="MSEHDTRRGTSAHTPDLDWSQVRETVLMLELAVGQIEAAMKEGH
                     SSVEVLTDSFTSMAGYMRMIAGALEQLPDTPATQNLKDNLIGHAGEVSGRIQRSIIAF
                     QFYDKLTQRLAHVSHSLEALSGLVVDQRKLYNPFEWVALQEKIRAKYSTREEVDMFNA
                     VMQGMPVKDALEKYKAGLKDKGDDVELF"
     gene            complement(8389..9438)
                     /gene="murB"
                     /locus_tag="PLH85_14950"
     CDS             complement(8389..9438)
                     /gene="murB"
                     /locus_tag="PLH85_14950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNB65914.1"
                     /translation="MTASADLLQQDVALDAFNTLRLPARAAWYARVDHVDGLRAVLDD
                     PRVAGLPRLVLGGGSNLVLAGDFAGLVLHVAFHGRSKVQEDADAYYVRAGGGESWHDF
                     VRWTLEQGWPGLENLSLIPGTVGAAPVQNIGAYGLEMAEHFYSLRAYDLATGKTVGFD
                     KRDCGFGYRDSFFKQAGAGRYLIAAVTFRLPKAWKPVTGYADVAAELAARGLVQPTPL
                     QISDAVIAIRRRKLPDPAEIGNAGSFFKNPVVDAATYARLAAAHPGLPHYPQADGREK
                     LAAGWLIDQAGWKGRRLGPVGCYDRQALVLVNHGGATGADVIAVARAVRADVAARFGV
                     DLEPEPVFAGTPPTP"
     gene            complement(9453..10301)
                     /gene="queF"
                     /locus_tag="PLH85_14955"
     CDS             complement(9453..10301)
                     /gene="queF"
                     /locus_tag="PLH85_14955"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987950.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNB65915.1"
                     /translation="MSTLPPPAHPDASPLGKPVAYAEHYDPSLLFPILRQGKRDELGL
                     AAGSLPFVGEDVWNAYELSWLNPRGKPEVAVARFVFPADSPCLVESKSLKLYLNAFNQ
                     TPVASADALAGMLAADLSAAAGAPVAVTVEPLSARPQRDTGYPEGVLLDSQDIAIDRY
                     TPAPELLRADTAAPAVSETLYSHLLKSNCLVTGQPDWAMLVVRYRGAPIDREGLLRYI
                     VSFRRHNEFHEQCVERVFTDILARCRPAELCVWARYTRRGGLDINPCRRTPGLPPPPL
                     LAEIRQ"
     gene            10455..>11345
                     /locus_tag="PLH85_14960"
     CDS             10455..>11345
                     /locus_tag="PLH85_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB65916.1"
                     /translation="MNALPIRYVDPVYRPPSEAQSLILPVTDGCSWNHCTFCEMYTDP
                     QKAFRARSEDEVLASIRATGERYGDQMRRVFLADGDALVLPTRRLLRILEAIRQHLPA
                     VHRVSSYCLARNLRRKTVAELRALREAGLALAYLGAESGDDTVLQRVNKGETFDSTRA
                     ALDLLGEAGIGRSVMILNGLGGPLFSRAHAEHSARLANATQPEYLSTLVVSFPQGEAR
                     FRAGFPEWAPLDPPALFREMETFLTALDLHRTVFRSDHASNWLVLKGTLGADKDRLLA
                     QVRAALERPDAAALRPAWARG"
BASE COUNT         1440 a         3852 c         4245 g         1808 t
ORIGIN      
        1 gatctgccag accccgtagg ccagaaagat ctcattcacc gcgaggccaa gcggacccga
       61 gatcgtcggg agccgccgcg gccgtgtcct caggggtgtc caaggtctcc aggcaggcgt
      121 caagggcctg gacgagggcg tcggccggtt cgggcagcac gtcgaaccag cgggtgatga
      181 ggcggcgttc ctcgtcgctg cgggcccaca gggcgagggc caggtcgcgc aggctgtcgc
      241 gcgcggggcg gccgaaggcg tcccgcaggg ccgggtcggc gtccgcgccg gtgcacaggg
      301 cgagggcgcg caggccgtcc aggtagtcgc ggacgcccac cggcacgcca ttggcctgca
      361 gctcgcggaa caggcccagg aggagcggcg gcagggggcg cgcggtggtg ttcaccagcc
      421 gcctccgtcc tcgtccttgc cggccttgta ggcgtcgatg tcggcgcgga gcttgaagag
      481 gaccgcggtg tccagggcgt ccagggcctt ggggtcgagg ctgtcttcct tgcggccctg
      541 ccagtgcagg gtgcgcaccc agtccaccag ttcgctggtg gcggggagct tgcggacgcc
      601 ctggcggcgg ccctgggcgc ggatctcgca aaagcgggcg acggccgcgg cgaggagggt
      661 gtcgcccacg ctttcgccgc ggggggcgag gttcttgcgc acgatgtcgg cgaggcgctc
      721 gggctggtct tcggggaagt ccagctgcag gtagaggcag cggcgcagga agggttcggg
      781 gagctgtttc tcccggttgc tggtgatgag gacgatgggc cgcgtgccgt ccttgggtgc
      841 gctgacgtgg cggccgaagc cgcgggcggc gcggcaggcg tcctcttcgt cgcggggcag
      901 gtcctcaagg tcgaattcga agcggtcgag gacgtccagc aggtcgttgg ggaagtcgat
      961 gtcggccttg tccacctcgt cgatgagcac cacctggggc gtgccggact ggatggcgtg
     1021 gccgaggggt tccaggctga ggtagggatg gacgtggcgg gccgtggtgt tgccggggat
     1081 ctgggcgtcc tgcaggcggc gcagggcgtc gaagcggtag agcaggtcct tggcctggct
     1141 ggtgctcttg acggtcatca cggtgaggcc gacgcccagc tcggtggcga tggcgctggc
     1201 gaggcgggtc ttgccgcagc cggcctcccc ttccacgagg atcgggcggc ccaggcggac
     1261 ggccagattg acgagggtgg ccgtggcggg ggcggccacg tagaagtcgg tggcggtgcc
     1321 ggtgaaggat ttgtacgggg tggcggacac ggtgggattc cttatcgggc gtgtttccag
     1381 aagccttcca ggttcaggcg gtagtagacg ttgggcggca ggccgtcggg cgtggtggcg
     1441 tgctcggcga tggcgtcgcg gcggtcgtcg tcgaggacga tgccggcgtc ctccaggtcg
     1501 gccagccagc tgcgcacctg gcgggcgttg aggggggcga gggcgggcag ggcgaggagc
     1561 tgacgatagt ccgtgtcggc gtcgtcggga tcggcagggc ggatgaactc gcccggcgcg
     1621 gcaggcagcg gggcgtgatc ggcgacgacg cagatgaagc ggctgcgccc gcccggcttg
     1681 agggcagcga gggcgtcggc caggggctgc cagaagcgct ggtggaacag ggcgaggcgc
     1741 tgcgcctcgc ggccgacggt gtggagcagc acgaccaccg tgcggcgctc cagacgtttg
     1801 gcgaggatgc gcgcgagggc gtcgacggtc ggttcgccca gcaggggttc gttgaggcgg
     1861 ctgcacagcc agcagatcag ggattcggcg ctgtcgggct cgctcgcctg gccggcgaag
     1921 acctccatgc cgtccttgcc cgtcgtccac tgggcgaggg cttccatgaa ctggggctgg
     1981 ccgtggtttt ccgggccgtg gacgaggaag gcgacggccc gctggtccct gcgctcgcgg
     2041 aaggtgtcga ggctgtcgcg gaaggcggtg aactggggtt tgcgcccgag gcggcccagc
     2101 tctgcggcgg gaatctcgcc ggtcgcctgg tagcggccgt cgagggcggc ttcgaggaga
     2161 tccaggttcc agtggttgat gcaggcctgg gccttgtcgc gcagctcgat gaggtcggcg
     2221 tagaagttga ggacgcggcc ctcctcggcg ctccagtcgg tcaccgcggc gacgaagcgc
     2281 tgctgggcgc tgcggtcggc ctcgacggct tccggtgtgg gcagggtgtg ccagcactgc
     2341 tcggcctgcg cgcgcaaaaa ggtgtcggcc tcgctgcccg gcttggcgcg caggatgaag
     2401 atgggcggcg tgaggtgggg ccagtgctcc accgcccagc ggaattcctg ttcggtgatg
     2461 gagatggtct ggccgggcgc gatgtggccg tagcggtggc cgaacaggcc gacgtaggcg
     2521 tcgcagtcga ggaggcggtc cttgcaggcc ttgaccgcgg tctcggcgtt gagggcccac
     2581 ttttcctgca ggaagatctt ggcggcccgc aggttgcctt ccaccgccgc cttgaccgcc
     2641 tcccggtagg cgaggcagtc ggtggccgtg gcgctcagga agatgcgctc gatcttgggc
     2701 aggtcggcgg cgcggggcat gggctcagga ggatggggcg ggcgctgggc ggggcgggtg
     2761 cctccggtct gcatagcata ggccagtcgc ccgccccgcg ccacgctgcc cagggcttat
     2821 gatagggcga tcttttgcgg agcgttcctc catgcgtatc ggcatcgacc tcggcggcac
     2881 caagatcgaa atcattgccc tcgacgcggc ggggcggacc ctcgcccggc gccgcgtggc
     2941 cacgccccag ggcgactatg cggccaccgt gcaggcggtg gcgggcctgg tggcggcggt
     3001 ggaaagcgag ctggggcagc gcggcacggt gggcgtgggc acgccggggg cgctgtcgcg
     3061 ggtcaccggg cgcatcaaga acgccaattc cacctgcctc aacggccagc ccctgcagga
     3121 cgacctgcag gccgccctgg gccgcccgct gcgcatcgcc aacgacgcca actgcttcgc
     3181 cctgtccgag gcggtggacg gcgcggcggc gggggcggag acggtgttcg gggtgatcct
     3241 cggcaccggc gtaggcggcg gcatcgtggt gcgcgggcag gcgctcaccg gcgccaacgc
     3301 gattgccggg gaatggggcc acaatccgct gccgggcggc gctgcgccag ggcccgcctg
     3361 ctactgcggg cgttcgggct gcgtggagac ctggctgtcc gggccggggc tggcggctga
     3421 ccacctgcgg gtgacgggcc ggacctgcac gccggaggcc ctggtcgcgg gcgcggcggc
     3481 gggcgacgcg gacagcatcg cgaccctcga ccgctacgcg gcacgcctcg cccgcgccct
     3541 ggcgacggtg gtgaatctgc tcgatccgga cgtcatcgtg ctgggcggcg ggctctccaa
     3601 tctggcgatg ctgtacgagg cggtgccgcg gctgtgggtg ccccacgtgt tctcggacgc
     3661 catcgccacg cgcctgtgcc gggcggttca tggggattcg tcgggtgtgc gcggggccgc
     3721 ctggctgtgg ccggattgag acaaatcccc gcttctgtcg cacaatgccg gatcatccag
     3781 ccgccttgcg gtacatcgcc ccctgaatgg tgtttgacct gtgcccgtct gcccgcccga
     3841 cctgatgtcc acccccctcg ccgtgtttgc cggtggattg cgtgccctgc cgccggaagg
     3901 ccagcagacc ggcatgttca aggcgccggt cgtcgggccg gtggcggtcg gcgtcgacgg
     3961 cctggcgggt gaccggcagg ccgaccgccg ggtccatggt ggccccgaga aggccgtgca
     4021 ttattttccg gccgagcatt acgctcacct gaccacgcgg gtgcccgccc tcgccgacgc
     4081 cctgcggccc ggcgtgctcg gcgaaaacct gtcggcccgg ggtctgaccg aagacacggt
     4141 gtgcatcggc gacgtgttcg cggcggggag ctgccgcctg caggtgagcc agccgcgcaa
     4201 tccgtgctgg aagatcaacc accgcctcga ccacgaccgc atgtcgcgca tcatcgtcga
     4261 cgacggctgc acgggctggt acttccgcgt cctcgcgccc ggcgtcctga cggcgggtga
     4321 ggtgctgcgc ctggtggagc gtccggcgcc gggcatgacc ctcgcccgcc tggcggccgt
     4381 caagggcgcg caccgtccgt cccccgacga gctgctcgcc gtggccgcag cgcccgggct
     4441 ggcaccggac gtcgcccgtc gtctccaggc gcggggcgca tggttgacca atagtggcgt
     4501 gccaccgtgc tgagtgccct tgactccctg tacagctcgg ctagaaagaa gcgtcatccc
     4561 accttcacat tcgggccctc caccgatgaa cccgtccctt gagcctccgc gccatcgcgc
     4621 cttctgtacg gccgagtttg ccggccgttt ctttctcgcc cagctggatg tgagtcagcg
     4681 catgggcaac ctgtatgccc tgctgggcat ggggctgacg gattgcgccc tgcgcagtca
     4741 cgtcctgcgg gccacccacg atgcggcata cacggtggac ctgaacgaac tggacagccg
     4801 caaggccctc ctgctggagg ccagtctgga aggcttcgcc accatcctgc gcaattttgc
     4861 cgaggaggtg gcggcggccc acgtgcgctg tctggagcag gccctcggac gggtgccctc
     4921 gggcgccccg tcggcgctcc cgccggcctt gctgcccttc ctgcagcccg atttcgccgg
     4981 cttcggccgc gccttcaatg aggccctgcg cgaacgggcc gaggggcgcg gctgaagggg
     5041 gcggccccga ccttgaggtt tgcccgcatt gacaggggca cgcgggcggc ggataagctc
     5101 tccggtccgg caggggcgtg accctgcgcc ggaccgcatt cctccgctcc gtaccgtcca
     5161 tgactcctgt cccgtccccg cccgcaccgc ctgtcgcacc gccgtcggcg cccttcctgg
     5221 tcagccggat cttcctgccc ttcgccctcg gctactacct gtcctacctg ctgcgcaccg
     5281 tcaacgcggt gatctcgccg gacctgaccc gcgagttggg cctcaatgcc gccgatctgg
     5341 gcctgctcac cagcacctac ttcttcgcct tcgggctggc ccagatcccg gtgggcatcg
     5401 ccctggaccg cttcgggccg cgtcgcgtcg aatccgccct gctgctgatc accgccctgg
     5461 gggcgacgct gttcgccctc ggcgaggatc tgccggtgct ggccggcgcc cgcgggctga
     5521 tcggcttcgg cgtgtcggcc tgcctgatgg gcggcctgaa gggcttcacc ctgtggttcc
     5581 cgcgggagcg cctggcctcc ctcaccggct acatcatgtc cggcggcgcc ctcggcgcgg
     5641 tgacggcctc ggcaccgctg gaggcggccc tgcccttcat cggctggcgt ggcgccttct
     5701 gggccgtggc ggtgctggcg gcggggattg ccgcgctgat cttctttgcc atgccggaaa
     5761 aatcctccgg ccacggtggt ggcgatctgc aggcggccct gcgcggcgtg aaggaggtgc
     5821 tctccgcacg gcgtttctgg agcttttccg ggcaggcagc cttcttcacc ggcggcttca
     5881 tggccctgca ggggctgtgg gcggtgccct ggctgatgca ggtgaatggc tattcccgcg
     5941 cggtggcggc ggatcacctg ttctggctca acctgggcat gctcgccggg cagctgtcca
     6001 tcggcgcctg ggcgacgcgc ctggcggccc gcggcatcac gcccctgcgc ctgatgcagg
     6061 gcggcctgtt cctgaccatg ctcgtcgagg cgctgatcat cgcccgcctc ggcccgaccc
     6121 agcccctgtg gtgcctgctg ggcatcgtct cggccaccgg cgcgcagatg tacggcgtgg
     6181 tggcggggct gttcccgctg catctgtccg ggcgggtcac taccagcatc aacctgatgg
     6241 cctttgtcgg cgcctttgcg gtgcagtggg gcctgggcgg gctgctggac ggcctgcgcg
     6301 ccggcggtat gagcgcggcc acgtcgctgg aggtggcctt cctgatcctg ctggcggccc
     6361 aggcggccag cttcgtgccc ctgtcacggg ccgggcgtta cacgccggtc cactgattcc
     6421 gcccgacccg atcgaccctg tggggtcttg cggtccggag gtaagcgttg tcatgatggg
     6481 ggcgggcgga cggtccgccc ttgtcctgcg agaagcccat gtcgtcgcgt caactgtatg
     6541 tccatcgtta tcccgccgcc catcccgaag gtcggccgac cctgctgttc gtccatggcg
     6601 cctattccga tggccgcctg tgggtggagc acttcatccc gtggttccgc gaagccggct
     6661 acacctgcct ggcggtggat ctgtcgggtc atgggcgcag cgaagggcgg gacgacctcg
     6721 acagcctggg tctggacgac tatgtggatg acgtggccca ggtcgttgcc acgctgcgcg
     6781 cgccgccggt gctgatcgcc cattccatgg gttccctggt ggcccagcgc tatgcgcagc
     6841 gcgcgccgct ggccgggatg gccctgctgg cgccggtgcc tcccaccggg acgggcggtt
     6901 cggctggccg gctggcgctg atgctgcccg agtttttcga ggagctgccc cgcgccatcg
     6961 ccggttcggc cggcccgcgg accctggagg tcatggccag ggtgtacttt tcgccggaga
     7021 tgcctgcccg cgagacggcg cactacctgc ccctgctgca ggcggaatcc gaacgggccg
     7081 tgctggaaat ggtcacgctg cccctgcgcg gtctgtcctg ccgtccgcgg ctgccggtgc
     7141 tggtgatggg cggcagccag gacgcggtgt tcccggcgtc gatgctgtgc ttcaccgcgc
     7201 tgccctggcg cgcgcaacag gacgtggtgt ccggtgccgg acacatgctg atgctggatc
     7261 cccaatggga agacgccgcc acgcggatcg ccgcgtggct ggagacgctg cccgcggcga
     7321 cgtgacgccg cgctgccggg gcgcatgcgg gccgctcagg cccggtccac catcacgtag
     7381 cgcttggtga cggtttccag cacctcgaac acgcccttga agccggcggg gatcacgcag
     7441 gcgtcgccgg ggccgaagtc ccgcgcgtgg ccggcgtcgt cgatgaggcg caggcgtccg
     7501 ctcagcacct ggaagaactc gtgccggccg gcatggaacg cgatgcgcca ggcgcccggc
     7561 tcgcaggtcc actcgccgat ggacagggcg ccgtcctcgg cgctgtagcg ttcggcggtg
     7621 aggcgccggg gcggcgggcc gagggcccgt tccggggccg ggcgatcttc gctggccgcg
     7681 ggcgcagggt cggcaaagtg gatcagggcg ggctgcgggg gggcgatcgg cgtgggcatg
     7741 ggcagaacga gaggtcggtc atcagaacaa ctccacgtcg tcgcccttgt ccttcaggcc
     7801 ggccttgtac ttttccaggg catccttgac cggcatgccc tgcatgaccg cgttgaacat
     7861 gtccacctct tcgcgggtgg agtatttggc gcggatcttt tcctgcaggg ccacccactc
     7921 gaaggggttg tacagcttgc gctgatccac caccaggccg gacagggcct ccaggctgtg
     7981 ggagacgtgg gcgagacgct gggtgagctt atcgtagaac tggaaggcga tgatggagcg
     8041 ctggatgcgc ccggagacct cgccggcgtg gccgatcagg ttgtccttga ggttctgggt
     8101 ggcgggcgtg tcgggcagct gttccagcgc gccggcgatc atgcgcatgt agccggccat
     8161 ggaggtgaag gagtcggtga gcacctccac cgagctgtgg ccttccttca tggccgcctc
     8221 gatctggccg acggccagtt ccagcatcag gacggtttcg cgcacctggc tccagtccag
     8281 gtcgggggtg tgggcgctgg tgccgcggcg ggtatcgtgc tcagacatgg gggctccgtt
     8341 ttccggtgtg aatggcatac cggcttttcg gcgcggcccc gaaaagcttt agggcgtcgg
     8401 cggcgtcccg gcgaacaccg gctccggctc cagatccacg ccgaagcggg ccgccacgtc
     8461 ggcgcgcacg gcccgggcga cggcgatcac gtccgccccc gtggcgccgc cgtgattgac
     8521 caggaccagg gcctggcggt catagcagcc caccgggccg aggcggcggc ccttccagcc
     8581 ggcctggtcg atgagccagc cggcggccag cttctcgcgc ccgtcggcct gggggtaatg
     8641 gggcaggccg gggtgggcgg cggcgaggcg ggcgtaggtg gcggcgtcca ccaccgggtt
     8701 cttgaagaag ctgccggcgt tgccgatctc ggccggatcg ggcagcttgc ggcggcggat
     8761 ggcgatgacc gcgtcagaga tctgcagcgg tgtgggctgc acgaggccgc gggcggccag
     8821 ctcggcggcc acatcggcgt agccggtgac gggcttccac gccttgggca ggcggaaggt
     8881 gaccgcggcg atcaggtacc gcccggcgcc ggcctgcttg aagaagctgt cccggtagcc
     8941 gaagccacag tcgcgcttgt cgaagcccac ggtcttgccg gtggccaggt cgtaggcgcg
     9001 caggctgtag aagtgctcgg ccatctccag gccgtaggcg ccgatgttct gcaccggcgc
     9061 ggcgcccacc gtgccgggaa tcagggacag gttctcgagt cccggccagc cctgttccag
     9121 ggtccagcgc acgaagtcgt gccaggattc gccgccgccg gcgcgcacgt agtaggcgtc
     9181 ggcgtcctcc tgcaccttgc tgcggccgtg gaaggccacg tgcagcacca gcccggcaaa
     9241 atccccggcc agcaccaggt tggagccgcc ccccagcacc agcctgggca ggcccgccac
     9301 gcgggggtcg tcgagcacgg cgcgcaggcc gtccacgtgg tccacgcggg cgtaccaggc
     9361 ggcgcgggcg ggcaggcgca gggtgttgaa ggcgtcgagg gcgacgtcct gctggagcag
     9421 atcggcggag gcggtcatgg cggcggggcg gcttattggc gaatctcggc gagcaggggc
     9481 ggcggcggca ggccgggcgt gcggcggcag gggttgatgt cgagcccgcc gcggcgggtg
     9541 tagcgcgccc acacgcacag ctcggcgggg cggcagcgcg ccaggatgtc ggtgaacacc
     9601 cgctccacgc attgctcgtg gaactcgttg tggcggcgga aggacacgat gtagcgcagc
     9661 agcccctccc ggtcgatggg cgcgccccgg tagcgcacga ccagcatcgc ccagtccggc
     9721 tggccggtga ccaggcagtt ggacttgagc aggtgggaat acagggtctc gctcactgcg
     9781 ggcgcggcgg tgtcggcgcg gagcagctcg ggcgccggtg tgtagcggtc gatggcgatg
     9841 tcctggctgt ccagcagcac gccctccggg tagcccgtgt cccgctgcgg gcgggcggac
     9901 aggggctcca ccgtcaccgc caccggcgca ccggccgcgg cagaaagatc ggccgcgagc
     9961 atgcccgcca gcgcgtcggc gctcgccacc ggcgtctggt tgaaggcatt gagatacagc
    10021 ttgagggact tggattccac caggcagggc gaatcggccg gaaacacgaa gcgcgccacg
    10081 gccacctccg gcttgccgcg ggggttcagc cacgagagct cgtaggcgtt ccacacatcc
    10141 tcgccgacga agggtaggct gccggcggcg aggcccagct cgtcacgctt gccctggcgc
    10201 aggatcggaa agagcaggct cgggtcgtag tgctcggcgt aggcgacggg cttgcccagg
    10261 ggcgaggcgt cgggatgggc ggggggcggc agtgtgctca tggccgccat tttatcggct
    10321 ggggcgccgc ggcggggcgc cgtcgtccga cgcggggccg ggcgcctggg tgcgcccggg
    10381 gcgtgccggt cgccccttga accccgcccc gcccgctccc acaatggcgc cgtcgcgccc
    10441 cacccgatct gcccatgaac gccctgccca tccgttacgt ggacccggtc tatcgcccgc
    10501 ccagcgaagc ccagtccctg atcctgcccg tcaccgacgg ctgctcctgg aaccactgca
    10561 ccttctgcga gatgtacacc gacccgcaga aagccttccg cgcccgcagc gaggacgaag
    10621 tgctggccag catccgcgcc accggcgagc gctacggcga ccagatgcgc cgcgtcttcc
    10681 tcgccgacgg cgacgccctg gtgctgccca cccgccgcct gctgcgcatc ctggaagcca
    10741 tccgccaaca cctgcccgcc gtgcaccggg tgtccagcta ctgcctcgcc cgcaacctgc
    10801 gccggaagac tgtcgccgag ctgcgcgccc tgcgcgaggc cggcctcgcc ctcgcctatc
    10861 tgggcgccga atccggcgac gacaccgtgc tccagcgcgt gaacaagggc gaaaccttcg
    10921 actccacccg cgccgccctc gacctgctcg gcgaagccgg catcggccgc tccgtgatga
    10981 tcctcaacgg cctcggcggc ccgctgttca gccgcgccca cgccgaacac tccgcgcgcc
    11041 tcgccaacgc cacccagccg gaatacctgt ccaccctggt ggtcagcttt ccccagggcg
    11101 aagcccgctt ccgtgccggc ttccccgaat gggcgccgct ggacccgccc gccctgttcc
    11161 gcgagatgga gaccttcctc accgccctgg acctgcaccg caccgtcttc cgctccgacc
    11221 acgccagcaa ctggctggtc ctcaagggca ccctcggcgc cgacaaggac cgcctcctcg
    11281 cccaggtccg cgccgcgctg gaacggcccg acgcggccgc gctgcggccg gcgtgggcgc
    11341 ggggg
//
LOCUS       DAOQMM010000117        11320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2639_length_11320_cov_45.607368, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000117 DAOQMM010000000
VERSION     DAOQMM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_11320_cov_45.607368"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLH85_14965"
     CDS             complement(<1..889)
                     /locus_tag="PLH85_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB65917.1"
                     /translation="MQSQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLVWPQLNVA
                     EWLHFGRLRPLHTNAVIFAFGGCALFATSYYCVQRTCHTRLFAPGLAAFTFWGWQLII
                     VLAAITLPLGFTSGKEYAELEWPIDILITLVWVSYAVVFFGTVATRKTTHIYVANWFY
                     GGFILTVALLHLVNSAEIPVSLTKSYSAYAGVQDAMIQWWYGHNAVGFFLTAGFLGMM
                     YYFVPKQAGRPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVFSLILL
                     APSWGGMINGIMTLSGAWHK"
     gene            complement(1096..2637)
                     /locus_tag="PLH85_14970"
     CDS             complement(1096..2637)
                     /locus_tag="PLH85_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65918.1"
                     /translation="MIGVAAADDGLFAEGRRLYMEGRRADGSALLARRDGDGRLQGRD
                     MACVQCHRRSGAGTVEGVVIVPSIQGRVLFNPGSPPPGHLPRRAAGMALQDYGFRTRP
                     AYDDISLARALREGIGAGGARFDGLMPRYDLSSRDIAALTAYLHSLDATPADGIDPQP
                     GGAIRLATVILPDADPSQSAAMRAVLSTCVAERSPRPGSGRRPWTLDEWALSGPPETW
                     RGQLEAFRMERPVFALLGGIGRDWAHVHAFCEANALPCLFPNTDAPGEQPPPHASMYL
                     SPGVGVEAGVIAADLDESGDMPERLVQVFQRRDIGRYGAAALASALPGIPNRAVSSGA
                     DPGWIRAGEVLVLWLAPTDLASFFMAVPEPPDGVRVYLSASLAGFDTGVDTSLIPPPW
                     RPAVRVVYPFDLPDLRLGRQVFNLGGWMAGHGLALVRSTARVQGDSFSACEVVARALY
                     TLPEHPTREVLLERVEEAYAGAVATAYPRFTLGPGQRFGSKGAYLLKIMPDGKWQPQG
                     AWRVP"
     gene            complement(2670..3332)
                     /locus_tag="PLH85_14975"
     CDS             complement(2670..3332)
                     /locus_tag="PLH85_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB65919.1"
                     /translation="MIDLFSRCPAQSLRLAARVLALCAVMSSTAVRADDPGHHVHQDH
                     HAHHLMPPPSAAVVRSEQKVVLPPVGQRSAEGVNKSFAEFVGEGRPVVVNFIYTTCTA
                     ICPPMTRVFAEVQERLGAERESVRLISVSIDPEQDTPRRLKAYGAQYGAVAPWVFLTG
                     SEAASTSIQQAFNVFRPDKMGHTPVTFIRRQPGESWIRLDGIASAERILAEVHGVAGG
                     RR"
     gene            3562..4155
                     /locus_tag="PLH85_14980"
     CDS             3562..4155
                     /locus_tag="PLH85_14980"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNB65920.1"
                     /translation="MNKKNTLLAALAVIVGVFILIISLPDRSDPPLPVPLVAAPGEPI
                     PGRVPETDRFRLHGIIGESAGHAGSALIAEGDGPALHVSEGGELRPGVRLERVLPDRV
                     LLRLDGVAAPIELTLAVTVASTPELLAPPPLATSTGSGGDHELPGPPRGSAPPIQPAT
                     LPRDRVAAPIDASGPPPGTGLTGPALSGPSPGGSTEK"
     gene            4409..6277
                     /locus_tag="PLH85_14985"
     CDS             4409..6277
                     /locus_tag="PLH85_14985"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HNB65921.1"
                     /translation="MKPNRRTLLKGGAAGLLSALIAPPGLAQTASSVLGASKGRQFSQ
                     LMPNPLDPANLFAPDMFGGTTYTLKIREFSANLGLVNPATGAPLPTTLWGYGTANQAP
                     TYPGRTFNVRQNKAITVRYQNELLNAAGPLPHRLPVDTTIDWANPGNLGGRAPVPLVA
                     HRHGGDQGTLSDGLPDAWSTPDANRDGMPDYRGRIYSAAYTFDNSQEAGHLWYHDHAL
                     GVTRLNVYMGLAGNYFIRDSNEDVLVRYGHLPAYPYEVPVCIQDRSFTSTGALNYPAT
                     DPANPSAPTPTHLPEFFGDIIVANGVPWPRMDVEPRVYRLRLLNGSDSRVYTLSLAAE
                     KFPNTPLTFWQIGTDLGLMNAPVALTALTLAPGERADVLVDFSRIAWWYHGKVIVRNG
                     ANTPFPNGAPPVAGLTDRVMAFSVTKPLGYYGFSFSALSALCLPGNLRPVLGPVPMPS
                     SANAKVRKLMLFEGTDAYGRLQTMLGTVNPAPGNPAGPGFGTFLYTDPVTENIKVGST
                     EIWEIHNTTVDAHPIHLHLANFRVLDRQPFTGTLVPKPMGPGGIASGGYLTQVNVGGT
                     RRPALANEQGRKDTVLAYPGEVTRIIATFNRPGEYVWHCHILSHEDHEMMRRFVVA"
     gene            6399..8024
                     /locus_tag="PLH85_14990"
     CDS             6399..8024
                     /locus_tag="PLH85_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB65922.1"
                     /translation="MLRISELRLPLDHPPEALRAALVQRLGVDDADLLAFTVFKRSYD
                     ARKAQHLTFIYIVDAELRDEAAVLARFAGDAHIKPAPDMTYRFVGTAPPDIAHRPIVI
                     GFGPCGIFAALILAQMGFKPIVLERGKAVRERTQDTWGLWRRNVLNPESNVQFGEGGA
                     GTFSDGKLYSQIRDPKHFGRKVLTEFVKAGAPPEILYVSKPHIGTFRLVGMVETMRAE
                     IQSLGGEIRFGQRVTDLLIEDGHVRGVRTATGDELRSDHVVLALGHSARDTFEMLHAQ
                     GVFMEAKPFSVGFRIEHPQSLIDRARLGPHAGHPELGAADYKLVHHARNGRTVYSFCM
                     CPGGTVVAATSEPERVVTNGMSQYSRNERNANAGIVVGIDPRDFPGSGPLAGIALQRE
                     LESRAYVLGGRSYEAPGQLVGDFLRGIPSTALGDVEPSYKPGVRLGDLSTALPDYAIA
                     ALREALPAFDRQIKGFNRYDAVLTGVETRTSSPLRITRGEDCQSLNVKGLFPAGEGAG
                     YAGGILSAGVDGIRVAEAVARDMLGEAVNAVED"
     gene            complement(8073..8354)
                     /locus_tag="PLH85_14995"
     CDS             complement(8073..8354)
                     /locus_tag="PLH85_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB65923.1"
                     /translation="MSVPSDFAAWRDAALADGFDEVLERRWPPDTLLETHTHPFALRA
                     VVVEGELWLTTAGESRHLEPGDCFELMRDEPHAERYGTQGAVYWVARRN"
     gene            8697..9059
                     /locus_tag="PLH85_15000"
     CDS             8697..9059
                     /locus_tag="PLH85_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNB65924.1"
                     /translation="MPKKARELAPIEVQRLTKPGMHTVGGVAGLYLQVLPTGARSWVL
                     RVMVAGKRRDMGLGGFPDVTLAQVREKAREARATIEKGIDPIAERAAARSALAASRGA
                     AVSFEDAARQFITAKSPE"
     gene            9019..9939
                     /locus_tag="PLH85_15005"
     CDS             9019..9939
                     /locus_tag="PLH85_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNB65925.1"
                     /translation="MLPASSLPRSLPSEKSAKHGQQWTNTLEAYAFPVVGKMACADIA
                     LPHVLKILEPIWMTKTETATRLRGRIESVLDWATVRGFRKGENPARWKGHLDKILPAP
                     GKVAKVEHHDAVALDDLGAFLHDLRQRDGIAARALEFLILTAARSGEVRGAIWAEIDI
                     SARVWTIPPERMKASKEHRVPLSDTAVRLLESLPRFAGNDLVFPSPRGLVLSDMTLTA
                     VMRRMKREEVPHGFRSTFRDWAAERTNYPREVAEMALAHTIGNAVEAAYRRGDLFEKR
                     ARMMQEWAKFCDTPVFSSAEVVPLQGKSAA"
     gene            9975..10574
                     /locus_tag="PLH85_15010"
     CDS             9975..10574
                     /locus_tag="PLH85_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65926.1"
                     /translation="MNRQQRDSLQANLSDKGRTATKSFETWLDGLSGKVYQSALSGLI
                     DIAVKASGSSKGLLLAQLLPDIEAAARQISRDVVAEAAGRKTYWPMQKELVELSRERL
                     ARKAACARKSRRGKPAAKWKVEAEGLISRAVKANLSNKVLIERLCARGVLENRDGEYW
                     WPGDVDEGEKRVANTRRFLEAELSKLKDRYKSKKNFIHH"
     gene            10729..10965
                     /locus_tag="PLH85_15015"
     CDS             10729..10965
                     /locus_tag="PLH85_15015"
                     /inference="COORDINATES: protein motif:HMM:NF017717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HNB65927.1"
                     /translation="MKSTPKAASTTALPAALAGFDSLPDSAFVRLPVVCGLFACSPAT
                     VWRRVKTGRIPAPVKIGENSTGWRAGEIRQALAL"
     gene            10981..11286
                     /locus_tag="PLH85_15020"
     CDS             10981..11286
                     /locus_tag="PLH85_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65928.1"
                     /translation="MSRVQPSNYPHERKRRHDVPIWPEWHLYEALKAAIPRTLDHDEY
                     SRRLRQTAAVLKVKPQTLRAALCRDGHCMGLRPSKLPNRFLVRDANAIDALLNGEVA"
BASE COUNT         2176 a         3987 c         3395 g         1762 t
ORIGIN      
        1 gcttatgcca ggcgccggac agggtcatga tgccgttgat catgccgccc caggagggag
       61 ccagcaggat gagggagaag accatgccga gggactgggt ccagtcgggc agcgcggtgt
      121 agtgcaggtg gtgcgggccg gcccacatgt aggtgaagat cagcgcccag aagtgcacca
      181 cggacagacg gtaagagtac accggacgac ccgcctgctt cggcacgaag tagtacatca
      241 tgccgaggaa gcccgcggtc aggaagaagc ccaccgcatt gtggccgtac caccactgga
      301 tcatcgcgtc ctgaacgcca gcataggcag agtaggactt ggtcagcgaa accgggatct
      361 cggcgctgtt aaccaggtgc agcagggcaa cggtcagaat gaagccgccg tagaaccagt
      421 tcgccacgta gatgtgggtg gtcttgcggg tggcaacggt gccgaagaac acgaccgcat
      481 acgacaccca cacgagggtg atcaggatgt cgattggcca ttcgagttcg gcatattcct
      541 tgccggaggt gaagcccaga ggcagagtga tcgcagcgag cacgatgatc agctgccagc
      601 cccagaaagt gaaggccgcg aggcctgggg cgaagagccg ggtgtggcaa gtacgctgca
      661 cacagtagta cgacgtggca aaaagcgcac agccaccaaa cgcaaagatc accgcgttgg
      721 tgtggagcgg gcgaagccgt ccgaagtgca gccattcggc aacgttcaac tggggccaca
      781 cgagctgtgc tgcgacgata acgccgacca gcatgcccac gatgccccaa accaccgtca
      841 tgatggcgaa ctgccgcacg actttatagt tgtaagtctg cgattgcatg tgagtcacct
      901 cattaagaaa aaacaaaaac ccccctgact gccgacgcat ggccggcagg cttccgcgga
      961 aggatacgcc gcgatcagag cgacattttg acacagatca accgggatgc gccccgggat
     1021 ggcaaggact cgttacacgg cgcgcctttc gggcggccgc cgatgatcgg cgcaatggcc
     1081 gagaaaccag tcccttcaag gcacgcgcca cgcgccttga ggctgccatt ttccatctgg
     1141 cattattttc aggaggtagg cacctttgga accgaatcgt tgcccgggcc cgagggtgaa
     1201 gcgggggtag gccgtcgcca ccgcgcctgc gtaggcttcc tccacccgtt cgagcagcac
     1261 ttcgcgggtc ggatgttcag gcagggtgta gagcgcccgc gccaccacct cgcaggcgga
     1321 gaacgaatcg ccctgaaccc gtgccgtact ccgcacgagg gccaacccgt gacccgccat
     1381 ccagccgcct agattgaaga cctgtcggcc gagccgcagg tcgggcagat cgaaggggta
     1441 gacaacgcga accgccgggc gccacggggg gggaatcagg gatgtatcga cgcccgtatc
     1501 gaatccggcg aggctggcgg aaaggtaaac gcggacgcca tcggggggct cgggcaccgc
     1561 catgaagaaa gacgccagat cggtcggcgc cagccacagg accagcacct caccggcgcg
     1621 gatccaaccc ggatcagcgc cggacgacac cgcccggttc ggaatgccgg gcaaggcgct
     1681 ggccagtgcc gccgcgccgt accggccgat atcccgacgc tggaacacct gtacgaggcg
     1741 ctccggcatg tcgcccgatt catccagatc tgcggcgatc accccggcct ccacgcccac
     1801 gcccggagac aggtacatcg aggcgtgcgg tggcggctgc tcaccgggcg cgtccgtatt
     1861 gggaaacagg cacggcaggg cattcgcttc acagaatgca tgcacgtggg cccagtcacg
     1921 cccgatgccg cccagcagcg cgaagactgg ccgttccatt cgaaacgcct ccagttgacc
     1981 gcgccaagtc tcgggcggcc cgctcagcgc ccattcatcc agcgtccagg gccgccgacc
     2041 actccccggg cgcgggctgc gttcggcaac gcacgtagac agcacggcgc gcatggccgc
     2101 cgactgcgac ggatcggcgt cgggcaggat caccgttgcg aggcgaatcg ccccgccggg
     2161 ttggggatcg atgccgtccg ccggtgtggc atccagactg tgcaggtagg ccgtcagcgc
     2221 ggcgatgtcg cgagaactca ggtcgtaccg cggcatcagc ccgtcgaacc gggcaccacc
     2281 cgcaccgatc ccttcgcgca gggcccgtgc gaggctgatg tcgtcgtagg cgggccgagt
     2341 ccggaatccg tagtcctgca gggccatgcc cgctgcgcgg cggggaaggt gcccgggggg
     2401 tggcgaaccc gggttgaaga gcacacgccc ctggatcgac ggcacgatga caacaccttc
     2461 caccgtgccg gcaccgctgc gtcgatggca ctggacacag gccatatcgc gcccctgcag
     2521 acgcccgtcg ccatcgcgcc gggccagcag ggccgaacca tcggcgcgac gtccttccat
     2581 atagaggcga cgtccctcgg cgaacaaccc gtcgtccgct gccgcaactc cgatcatgcc
     2641 cgcgaacagc agcggtcccc accaccgcat caacgccgtc cgcccgccac gccgtgaacc
     2701 tcggcaagaa tccgttctgc ggatgcgatt ccgtcgagtc ggatccacga ctcacccggc
     2761 tggcgccgga tgaaggtgac gggcgtgtgc cccatcttgt ccggacggaa cacattgaac
     2821 gcctgctgaa tcgacgtgct cgcagcctct gaaccggtca gaaagaccca tggcgcaacg
     2881 gcgccgtact gcgcgccgta cgccttcagg cgccgcggcg tgtcctgctc gggatcgatg
     2941 gacacggaaa tcaggcgcac ggattcccgt tccgccccga gccgctcctg cacttcggcg
     3001 aacacccggg tcatgggcgg acagatcgcg gtacaggtcg tatagatgaa gttcacgacc
     3061 actggacggc cctcacccac aaactctgca aaggacttgt tcacgccctc cgcgctgcgc
     3121 tggccgaccg gcggcaacac caccttctgc tcggaccgaa ccacggcggc cgagggcggt
     3181 ggcataaggt gatgggcgtg gtggtcttgg tggacgtggt gccccggatc atccgcccgg
     3241 acggcggtgg aggacatcac tgcgcacagc gccagaacac gtgccgccag gcgcagggac
     3301 tgcgcgggac atcgggagaa caggtcgatc atgatggggt tatccatggg aaggaggcgt
     3361 gcggcgggga cattccccgc ccgctggccg gattgtcccg agccagtccc tgcacgcccc
     3421 gtgacaggcg ccacattgtt cggtcgaccc gcggcaaatc ccgttaaatt tgcttacacg
     3481 cacgaacatt taatgatttt ggaaattctg ccggtggtac ggtgagggca tctcctttga
     3541 atctgccaac gccacccgcc catgaataaa aagaacaccc tactcgcagc cctggccgtc
     3601 atcgtcggcg tattcatcct gatcatcagc ctgccagacc gttcggaccc acctctgcct
     3661 gttccgctcg tggccgcccc cggcgaaccc atccccggac gggtaccaga aacggatcgc
     3721 ttccgacttc atggcatcat cggagaaagc gcggggcatg cgggttcggc cctgattgcc
     3781 gaaggcgacg gcccggccct tcatgtgtcc gaagggggcg aactccgacc gggtgtgcgc
     3841 ctcgagcggg tattacccga cagggtgctg ctccgcctcg acggggtcgc cgcccccatc
     3901 gagctcaccc tggcggtcac cgtagccagc acgccggaac tcctcgcgcc tccgccgctc
     3961 gccacgtcga ccggctcggg cggcgatcac gaattgcctg gtccaccgcg tggcagcgca
     4021 ccgcccattc agcctgccac gctcccgcgc gaccgtgtgg ccgcccccat cgatgcctcc
     4081 ggtccgccgc ctggcaccgg cctcacgggc ccggccctca gtggccctag cccgggtggc
     4141 tcgacggaaa aatagtcgat tccgccaaac catcgcgaaa gttcgcaaga aattctcaca
     4201 ccgctgggaa tttcaccttt attcacccaa attcatcaaa aacagtcgga ccatttgtaa
     4261 ccgaacattt ccgcccaagc gcagcgtcct cgccattcga cgcacaccac gcacgtgccc
     4321 cacaccacgc acgtgcccgt ctcgccgacg gtggacgtgc gacgggcgcc actacgccac
     4381 acccctcctc cttgcaaagg atttcacgat gaaaccgaac agacgaaccc tcctgaaagg
     4441 tggcgcggcc ggcctgctct cggcgctgat cgcaccgccc ggcctcgccc agaccgcgtc
     4501 ctccgtgctc ggagccagca aaggccggca attcagccag ttgatgccca accccctgga
     4561 tcccgccaac ctgttcgcgc ccgacatgtt cggcggcacc acctacaccc tcaagatccg
     4621 ggaattcagc gccaatctgg gtctggtcaa tcctgccacg ggcgcccccc tgcccacgac
     4681 gctgtggggc tatggcacgg caaaccaggc gccgacctat ccgggtcgga ccttcaacgt
     4741 gcgccagaac aaggccatca ccgtccgcta ccagaacgaa ctgctcaacg ccgccggacc
     4801 actgccccac cgtctgccgg tcgacaccac catcgactgg gccaatccgg gcaacctggg
     4861 cggccgcgcc ccggtaccgc tggtcgccca ccgccacggt ggcgaccagg gcaccctcag
     4921 cgacggcctg cccgacgcct ggtccacacc cgacgccaac cgggacggca tgcccgacta
     4981 ccggggccgc atctacagcg cagcctatac ctttgacaat tcccaggagg ccggccacct
     5041 ctggtaccac gaccatgcgc tgggcgtgac ccgcctcaat gtgtacatgg gtcttgccgg
     5101 aaactacttc atccgcgaca gcaatgagga cgtcctggtc cgctacggcc acctgccggc
     5161 ctatccttac gaagtgccgg tctgcatcca ggaccgttcg ttcacgtcca ccggcgccct
     5221 gaactacccg gccaccgatc cggccaaccc gtccgcaccc acaccgacgc atctgcctga
     5281 attcttcggc gacatcatcg tggccaacgg cgtgccctgg ccgcgcatgg acgtggaacc
     5341 ccgcgtctac cgcctgcgcc tgctcaacgg ctccgactcc cgcgtctaca ccctcagcct
     5401 ggccgccgaa aaattcccca acacgccgct caccttctgg cagatcggca ccgacctggg
     5461 cctcatgaac gccccggtcg ccctgaccgc cctgaccctc gcgccgggtg agcgcgccga
     5521 cgtgctggtg gatttctccc gcatcgcctg gtggtatcac ggcaaggtga ttgtgcggaa
     5581 cggcgccaac acgcccttcc ccaacggcgc accgccggta gccggactca ccgaccgggt
     5641 catggcgttc agcgtcacca agcccctggg ctactatggc ttcagcttct cggccttgtc
     5701 ggccttgtgt ctgcccggca acctgcggcc ggtactgggc cccgtgccga tgccatcatc
     5761 ggccaacgcc aaggtacgca agctcatgct gttcgaaggc accgacgcct acggccgcct
     5821 ccagaccatg ctcggcaccg tcaatccggc gcccggcaat ccggccggcc ccggattcgg
     5881 caccttcctg tacaccgacc cggtcaccga gaacatcaag gtgggcagca ccgagatctg
     5941 ggagatccac aacaccaccg tagacgccca ccccatccat ctgcatctcg ccaacttccg
     6001 cgtcctcgac cggcagccct tcaccggcac cctcgtcccc aagcccatgg gtccgggcgg
     6061 aatcgcctcc ggcggctacc tcacccaggt caacgtcggc ggcactcggc gcccggccct
     6121 cgccaacgag caggggcgca aggacacggt gctggcctat ccgggcgagg tgacccgcat
     6181 catcgccacc ttcaaccggc cgggtgaata cgtgtggcac tgccacatcc tgtcccacga
     6241 agaccacgag atgatgcgcc gctttgtcgt tgcctgacgg ccctccgggc ttggcgcgcg
     6301 caccgctgcg ccaggcccgc acggaaccgg gccggcccga tccgggctaa aatcgcgtcc
     6361 cgatcgcctc ccgcgggccg tccgcccgcc ctgttccgat gttgcgcatc tccgaactcc
     6421 ggctccccct cgaccatccg cccgaggccc tgcgcgccgc cctcgtgcag cgtctcggcg
     6481 tggacgacgc ggacctgctc gccttcaccg tcttcaagcg cagctacgac gcccgcaagg
     6541 cccagcacct caccttcatc tacatcgtcg acgccgaact gcgcgacgag gccgccgtgc
     6601 tcgcccgctt cgccggtgac gcccacatca agcccgcacc ggacatgacc taccgcttcg
     6661 tcggcacggc gccgccggac atcgcccatc gccccatcgt gatcggcttc ggcccctgcg
     6721 gcatcttcgc cgcgctgatc ctcgcccaga tgggcttcaa acccatcgtg ctggaacgcg
     6781 gcaaggccgt acgcgaacgc acccaggaca cctggggcct gtggcggcgc aacgtgctca
     6841 acccggaatc caacgtccag ttcggcgaag gcggcgccgg caccttctcc gacggcaagc
     6901 tctacagcca gatccgcgat cccaagcatt tcggccgcaa ggtcctcacg gaattcgtca
     6961 aggccggcgc cccgcccgag atcctctacg tctccaagcc ccacatcggc accttccggc
     7021 tggtcggcat ggtcgagacc atgcgcgccg agatccagtc cctgggcggc gagatccgct
     7081 tcggccagcg cgtcaccgac ctcctcatcg aagacggcca cgtgcgcggc gtgcgcaccg
     7141 ccacgggcga cgaactgcgc agcgaccacg tcgtcctcgc cctcggccac agcgcccgcg
     7201 acaccttcga gatgctccac gcccagggcg tgttcatgga agccaaaccc ttctcggtag
     7261 gcttccgcat cgagcatccc cagtccctca tcgaccgcgc ccgcctcggc ccccacgccg
     7321 gccacccgga actgggcgcc gccgactaca agctggtgca ccacgcccgc aacggccgca
     7381 ccgtgtacag cttctgcatg tgccctggcg gcaccgtggt ggccgccacc tccgagccgg
     7441 aacgggtcgt caccaacggc atgagccagt attcccgcaa cgagcgcaat gccaacgccg
     7501 gcatcgtggt gggcatcgac ccgcgcgatt tccccggcag cggccccctc gcgggcatcg
     7561 ccctgcagcg cgagctcgaa agccgcgcct acgtgctggg cggacgcagc tacgaagccc
     7621 ccggccagct cgtaggcgac ttcctgcggg gcatcccgtc caccgccctg ggcgacgtgg
     7681 agccttccta caagcccggc gtgcgcctgg gtgacctgtc caccgcactg cccgactacg
     7741 ccatcgccgc cctccgtgaa gccctccccg ccttcgaccg gcagatcaag ggcttcaacc
     7801 gatacgacgc ggtcctcacc ggcgtcgaga cgcgcacctc ctcgcccctg cgcatcaccc
     7861 gcggcgaaga ctgtcagagc ctcaacgtga aaggcctctt ccccgccggc gaaggcgcag
     7921 gctacgccgg tggcatcctc tcggcgggcg tggatggcat ccgggtggcc gaagccgtgg
     7981 cgcgggacat gctgggggag gcggtgaatg cggtggaaga ctgaacgcag gacaatcccg
     8041 accaccaggt cgccccatgc cggccggcga tctcagttcc ggcgcgccac ccaatagacc
     8101 gcgccctgcg tgccatagcg ttccgcgtgc ggctcgtccc gcatcaactc aaagcaatca
     8161 ccgggctcca ggtggcggga ttcacccgcc gtggtcagcc acagctcgcc ttccacgacg
     8221 accgcccgca gggcaaacgg atgggtgtgg gtctccagca gcgtatcggg aggccagcgc
     8281 cgctcaagca cttcgtcgaa tccgtcggcc agtgcagcgt cacgccacgc agcaaaatca
     8341 gatggaacgc tcatgccccc tcctccgcga caccccgcat gtccgtgatc cccaccgcgc
     8401 tcaggcttcg gaccacccgc aggcagcggg acgcgatgcc tggcacgaca gtgcgcatcg
     8461 accggatcgt ccatcgggta cgcgcatatc gggccccaac gggaatgaca ccccagcggc
     8521 gtctggcgga agcggtgtcc gccgggatcg aacttctttt cgcgtcaccg cgtatcaaag
     8581 tttgttctat cccttttact cattggaaat cgttgtatca ctccgcatcg tgcgctatcg
     8641 tgcaactgca tccacgttga tgggtgcaat gatggaatga caaacgaggc ggagaaatgc
     8701 ccaagaaagc ccgagaactg gcccccattg aggtgcaacg actaaccaag cccggaatgc
     8761 acaccgtcgg gggcgtggct gggctatacc ttcaagtcct gcccacaggc gcgcggtctt
     8821 gggtgctgcg cgtgatggtt gcggggaagc ggcgcgacat ggggctgggt ggctttcccg
     8881 acgtgacctt agcgcaggtc cgcgagaagg ccagagaggc ccgcgcaacc atcgaaaagg
     8941 gtatcgatcc cattgcggag cgggctgcag cccgcagcgc cctggcggcc tcgcgtggcg
     9001 cggccgtgag cttcgaggat gctgcccgcc agttcattac cgcgaagtct cccgagtgaa
     9061 aaaagcgcca agcatgggca gcaatggaca aacacccttg aggcctacgc cttccccgtc
     9121 gtgggcaaaa tggcctgtgc agacatcgcc ctgccccatg tgctgaaaat ccttgagcca
     9181 atctggatga ccaagaccga aacggcaacg cgcttacgcg ggcgcatcga gtcggtgctt
     9241 gattgggcga ccgtgcgagg gtttcgcaag ggcgagaatc cggcccgctg gaaggggcac
     9301 ctggacaaga tactgccggc ccccggcaag gtggcgaagg ttgaacacca tgacgccgtg
     9361 gccttggacg acctaggcgc attccttcac gaccttcgcc agcgtgacgg catcgcggcg
     9421 cgggcgctgg aatttctgat cctgaccgct gcacgttccg gcgaggtgcg cggcgcgatc
     9481 tgggcagaaa tcgacatatc cgcgcgagtc tggacgattc cgcctgagcg catgaaagcc
     9541 agcaaggaac atcgcgtccc gctttccgat accgccgtgc gcctgctgga atccctgccc
     9601 cgctttgccg ggaacgatct ggttttcccc tcgccccgcg gcctcgtgct gtccgacatg
     9661 accctaaccg ccgtcatgcg ccgcatgaag cgcgaagagg tgccgcacgg cttccgctcg
     9721 accttccggg attgggcagc cgagcgcacg aactaccccc gcgaagtggc cgagatggcg
     9781 ctggcccaca ccatcggcaa cgctgtagaa gcggcctacc gacgcggcga cctcttcgag
     9841 aagcgggcgc ggatgatgca agagtgggcg aagttctgcg ataccccagt attttcttcg
     9901 gctgaggtcg tacctctcca aggcaagtca gcggcgtgat ccacatcaaa ctgcggaagc
     9961 ctcgacatag aaacatgaat agacaacaac gtgattcgct acaagcgaac ctctcagaca
    10021 aagggaggac ggccacgaag agctttgaaa cttggctcga cgggctatcc ggcaaggtgt
    10081 accaatcagc tttgtccggt ctgattgaca tcgcggtgaa ggcatcaggt tcgtccaaag
    10141 ggctgttgct tgcgcagctc ttgccagata tagaagccgc agccaggcag atttctcggg
    10201 atgtggttgc tgaggctgca gggagaaaaa cgtactggcc gatgcagaaa gagctcgtgg
    10261 agctatcgcg agagcggctc gccaggaagg ccgcttgtgc tcgaaagagc agacgcggca
    10321 agcccgcagc caaatggaaa gtcgaggctg aagggctcat ctctcgggcc gtcaaggcaa
    10381 atttatcgaa caaagtcctg atcgagcgcc tgtgtgcccg tggggtcttg gaaaatcgcg
    10441 acggcgaata ctggtggccg ggagatgttg atgagggcga aaagcgagtc gccaatacca
    10501 ggcgatttct tgaggcagaa ttatcgaaac tgaaggacag atacaaaagc aaaaaaaatt
    10561 ttatccatca ttaacagtca cttaccactc gaactcagca taacctgttg gcgatctagc
    10621 cgctgatcgc caacggacgg ccagagcatc aagcctcaag ccgggccatc agatattgcg
    10681 gaatctcact gttggcccgc ctgtcatcaa cacagacacg aggcgacaat gaaatccacc
    10741 cccaaagcgg catcgaccac cgcgctcccg gcagccctcg ctgggttcga ctccctccct
    10801 gactcggcat ttgtcagact cccagtcgtc tgcgggctct tcgcctgctc cccggctacc
    10861 gtgtggaggc gcgtaaaaac cgggcgaatc cccgctccgg tcaaaatcgg cgaaaacagc
    10921 actggctggc gggccggcga gatccgccaa gcgctggccc tctgactgga gtctccggaa
    10981 atgtcgagag tccagcccag caactatccc cacgagcgga agcgacgcca tgacgtacca
    11041 atctggccgg aatggcacct atacgaggcc ctcaaggccg cgatcccgcg caccctcgac
    11101 cacgacgagt actcccgccg cctccggcag acggccgccg tcctgaaggt caagccgcag
    11161 acgttgcgcg ccgccctgtg ccgcgacggc cattgcatgg gcttgcgccc gtccaagctg
    11221 cccaaccgct ttttggtgcg ggacgcaaat gcaattgacg ctctgctgaa tggcgaggtg
    11281 gcctaaatgt ccgcctccag acgtgacaac gcccgcggcc
//
LOCUS       DAOQMM010000118        11242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2671_length_11242_cov_47.772772, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000118 DAOQMM010000000
VERSION     DAOQMM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2671_length_11242_cov_47.772772"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLH85_15025"
     CDS             <1..1209
                     /locus_tag="PLH85_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNB65929.1"
                     /translation="PGPATPAEGRPGETLTHDARDRRRQARREDMPDGRDALILPFRA
                     ESLLSFADYVKVNRAHDDDADDDPGRVAAEMDTVSVAQDGQDTASKVRFDLDLPSAAQ
                     DDIPLTTGILLPEWDWKTQQLRPDLCSLSVLDPRDAEPCALPPRLIRPARRLRRQFEA
                     LTPARRWLKNQPDGSDLDIDACVRAFADRMTGHSVSASGGYLACERRERDLCCLVLAD
                     LSLSTDAWVSNEARVIDVIRDSLWLFTEALAATGDPFGLYGFSSRKREHIRFHRIKDF
                     DERPGDRVRGRIAAIKPGFYTRMGAAIRYATRLLARRPESVRLLLILSDGKPNDVDHY
                     EGRYGIEDTRMSLRAAREEGVRPFCVTIDREGAGYLPHLFGPAGYTILRKPADLPARL
                     PLLYAQLTGA"
     gene            1342..3045
                     /locus_tag="PLH85_15030"
     CDS             1342..3045
                     /locus_tag="PLH85_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB65930.1"
                     /translation="MARLFSRTLRRLGAACSVLAPLAGLLAGGGLAQAQTSYSSGYWY
                     GNASPVSVLTHHNDGWRTGWNYAETTLTPSNVAATGGSKLFGLQGSVDLDGLVEVQPL
                     VVPNAYVYGDPNAGRHDVVYVATANNTVYAIDPTTRKVLRSRNLGTPMSAFPGCEDND
                     KIIGVNSTPVIDPSTNSLYLIAHVRVGSGSEMHLHRLSLSSLKDIVAPVTIAAKAPLT
                     NGSTATFDANYQRQRTALLLANGRVMAAFTSHCDIRADVARGWVMQWDAQTLDKARSY
                     VDFSGRKSVFLTDTQATGPSNFFLATSWMSGAGPAVDEWGNVYFATGNTDPGALRSDG
                     TGYVEDGVTNFGQSVVKLSPWDSRVLSYFTPANVMQLDNDDLDLGAGGVLLVPSYTDR
                     LALQAGKDGVLRLMNRDSLGGHTPGGPDKVLAATQIGPCWCTPSYFNDGTPKVVSSGG
                     TTLGVYAIRTKPYGLVKQAGVEINSGQFGGFFTSVSSNGAQNAIIWAVGRPQTIPGDL
                     YLHAYSAKPVNGSLPLLYTTTAGTWNSQGNANTVPTVANGRVYVAGYKKLSIYGLGGQ
                     R"
     gene            3062..3373
                     /locus_tag="PLH85_15035"
     CDS             3062..3373
                     /locus_tag="PLH85_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65931.1"
                     /translation="MKTTRILILAAALGGVTAPVLAEAPNAVTGVVRSIAGATLQVEL
                     RGGTALQVEQSDALAAHQVAPGIKVGTAVHATGTRSGNLLRAETVTRAKPAPGTWSPD
                     R"
     gene            complement(3572..4369)
                     /locus_tag="PLH85_15040"
     CDS             complement(3572..4369)
                     /locus_tag="PLH85_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65932.1"
                     /translation="MYVSDLVSHLQTLIACSALDFVFQPIFDLRRREITGYEALMRGP
                     DDSPLNTPAQLLQVAREAGLELALERLASRLALEAFAAVELPGSLFLNLSLAALIDQA
                     RTDGVVALAGRAGLPPTRLVVEPLPDAPATGCAATACASLDAQGVRIAVHGALPPQER
                     AGFRPAFLKLGPAAFHRIHADPLRRLDLASVLAEAARRGVQVVAEGVEDARELATLFD
                     LGCDCAQGHLLGRPAADPLRRPSRGVDGLLQARAILQPRPADATLPV"
     gene            complement(4501..5385)
                     /locus_tag="PLH85_15045"
     CDS             complement(4501..5385)
                     /locus_tag="PLH85_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB65933.1"
                     /translation="MNWDDLRIAAAIGRAGTLAAAARRLGIDQTTVARRLRALEDALG
                     TPLFERGEGGWQPTAPGSEVLARAWRMEEDAADITRIADAGARSVSGRVHLTSVGPIV
                     ADYLAPRLASLMSTYPALDVDLIARNDNLSLARREADIALRLGRPADGDFLIRKLADV
                     GFAVYAPADTPIDPVAWVAYGEDLDHTPEMRWLAARLNGPAGNTAARIRLRCDSLRGL
                     TRAIAAGIGCGVLPCFIGDACSDLRRVPTDALPVRELWQLVHRDARRLERVDAVSTWL
                     AERFAADADLFRGLHPPA"
     gene            5507..6106
                     /locus_tag="PLH85_15050"
     CDS             5507..6106
                     /locus_tag="PLH85_15050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNB65934.1"
                     /translation="MADAFTLRALMGKPVTPTRLSESVLILIDAQNAYRHGPLTLSGI
                     EPALASCARLLARARALGAPVIHIQHDAGPGTPFDPLSESGAFIDVVAPRPGEAVVVK
                     HFPNAFVGTDLEARLQALGARNLVIAGFMTHNCVNSTARGAYNLGYRFTVPGNATATR
                     ALPAIGGGQLDAATLQAALLVSLTDVFGVVVADEAEIPD"
     gene            complement(6167..6604)
                     /locus_tag="PLH85_15055"
     CDS             complement(6167..6604)
                     /locus_tag="PLH85_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB65935.1"
                     /translation="MPTRPVSQVVSRRSFLKAPSTTSAAEAARMMRDQHKSTVTVVNA
                     TGRVEGICSERDLVQKVMAAGRDPAVTPLAEIMTPNPQTIPADKPFCHALHIMFDGGF
                     RHLPVVDEAGRPIGIVSVRDALGLEMIQFGHEMEQRDTLTEIL"
     gene            6830..8764
                     /locus_tag="PLH85_15060"
     CDS             6830..8764
                     /locus_tag="PLH85_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB65936.1"
                     /translation="MTAPPVAASARRVLSLIPPMTQLNTPYPSTAYLTGFLRSRGVDA
                     VQDDLALKLVLELFSPDGLEAIRARAEALPARRQSPAVQGFLAQFARYRATVGPAVAY
                     LQGRDPTVAHRIASRQFLPEGGRFAALDQYVDPDGGDPLAWAFGALGTADKARHLATL
                     YLNDLADVLRDAVDPRFEFVRYAESLARSQPGFEPLARALAAPPTLVDEVLESLAAEA
                     LTRHAPQVVLLSVPFPGSVYGAFRIAQAIKARAPQVVTVLGGGFANTELRELSEPRVF
                     DFFDYVTLDDGERPLLALLEHLDGRRGPSRLVRTYRRDEAGMVRYEHWVEPDVPFAEV
                     GTPTWDGLPLDRYLSVLDMLNPMHRLWSDGRWNKLTVAHGCYWKKCSFCDVSLDYIGR
                     YDGAAAALLADRIDAIVTETGESGFHFVDEAAPPKALRALADELLQRGRAISWWGNIR
                     FEKSFTPELCERLAESGCIAVSGGLEVASDRLLKLMKKGVSVDQVARVTRAFSDAGIL
                     VHAYLMYGFPTQTVQDTVDALEYVRQLFDAGCIQSGFFHRFACTVHSPVGLNPAEYGV
                     TLKALPPVSFARNDVEFVDPSGVDHDALGRALNKALYNYMHGIGLDEDVRAWFEGRVP
                     RTRVPRRFVERALGVAPARA"
     gene            complement(8771..>11242)
                     /locus_tag="PLH85_15065"
     CDS             complement(8771..>11242)
                     /locus_tag="PLH85_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65937.1"
                     /translation="WRDFVTPMMARNPGTQNFGWSPLVTDAQRDAFEARVRAAGRPDF
                     RILGRNVAGQTFPAARKAEYLPILFVEPLAENRSVLGLDPLVLDRTASAVQRSRKSGL
                     PIASESIRLVQETGEQRGIVVYQAVLAGSPPHMTGLVSAVFRMDDAMQAILGHRRTGE
                     LDVCLSEPRTGASERLYGPAGCEQAGWAGKRLNWETPLTFAGRTWLLRVGATSAYMNT
                     VGNWGLWLMLALGLLSTGILGAFLLLTSGRTRRVESLVDERTRQLAAATDTLRRQQQE
                     LAQAQRIARLGSWETLPGSDELRCSDELMAMLTLDGSTPIRTRQLIERLHPDDRPRFL
                     HALEQVDAMPHRIDLDCRLARDGTPMEILHFRLESEGHGPALRIRGTAQDVTSARAAE
                     ARIARLAHFDVLTGLPNRTQWNERAHSELRAAARHGDILAVLFLDLDHFKAVNDSLGH
                     PVGDQLLSAVARRLSGCLRENDFLARLGGDEFVVLLPRLSHREDAALVARKLIAALEH
                     PVVMGAHELTVSVSIGIALHPDDGSDVDTLLKHADLAMYRAKDQGRNAYHYFLPDMDV
                     RAMERLLLETALRRAVERDELRLHYQPQLRTRDSRVTGCEALVRWQHPELGLLPPAQF
                     IGVAEASGIIHALGAWVLEHACRQQVIWATAGRPLIMAVNISALQFRQAHFYGTVKRI
                     LEDTGIDPAHLELELTESALMQLTPEVLERMHALRALGVNLALDDFGTGYSSLSHLKR
                     LPIRRLKLDRSFVEALPADLEDAAIASATLSLAQDLGMEVVAEGVESPSQVDFLTRRG
                     CPLLQGFLFSEPLPAAEFDAWLLAR"
BASE COUNT         1638 a         3849 c         4037 g         1718 t
ORIGIN      
        1 ccagggccgg cgacccccgc cgagggccgt cccggcgaga cgctgaccca cgacgcccgc
       61 gaccgccgcc gtcaggcccg ccgcgaggac atgcccgacg gccgcgacgc cctgatcctg
      121 cccttccgcg ccgagagcct gctgtccttt gctgactacg tgaaggtgaa ccgcgcccat
      181 gacgacgatg cggacgacga tcccggccgc gtcgccgcgg agatggacac cgtgtcggtg
      241 gcccaggatg gccaggacac ggcgtccaag gtgcgcttcg acctggacct gccctccgcc
      301 gcccaggacg acatcccgct gacgaccggc atcctgctgc ccgaatggga ctggaagacc
      361 cagcaactgc ggccggacct gtgcagcctg agcgtgctcg acccccgcga tgccgaaccc
      421 tgcgccctgc cgccgcggct gatccgcccg gcgcgacgcc tgcgtcgcca gttcgaggcc
      481 ctcacgccgg cgcggcgctg gctcaagaac cagcccgacg gcagcgacct ggacatcgac
      541 gcctgcgtgc gcgcctttgc cgaccgcatg accggccatt cggtcagcgc cagcggcggc
      601 tacctggcct gcgagcgccg cgagcgcgac ctgtgctgcc tggtgctcgc ggatctgtcc
      661 ctgtccaccg acgcctgggt gagcaacgag gcgcgggtca tcgacgtgat ccgcgacagc
      721 ctgtggttgt tcaccgaggc cctcgccgcc accggcgatc ccttcggcct ctacggtttt
      781 tcgtcgcgca agcgggagca catccgcttc caccgcatca aggatttcga cgagcggccc
      841 ggtgaccggg tgcgcgggcg cattgcggcg atcaagccgg gcttctacac gcgcatgggc
      901 gcggccatcc gctacgccac ccggctcctc gcccggcggc cggaatccgt gcgcctgctg
      961 ctgatcctgt ctgacggcaa gcccaatgat gtggatcatt acgagggccg ctacggcatc
     1021 gaggatacgc gcatgagcct gcgcgccgcc cgcgaggagg gcgtgcggcc cttctgcgtc
     1081 accatcgacc gggaaggggc gggctacctg ccccacctgt ttggcccggc cggctacacc
     1141 atcctgcgca agccggcgga tctgccggcc cgcctgcccc tgctctacgc ccagctcacg
     1201 ggcgcctgat ggcgccggga atgccggtgt gtgacaggtg gcacgggcgg tacgtgtttc
     1261 tggcaaatct gacgatttgt aactggttgc gacgcgcggg ccgtcctagc atgggctatt
     1321 gccattttta tggaatcagc catggcccgt ttgttctcac gcaccctgcg ccgcctcggc
     1381 gcggcctgtt ccgtcctcgc tcccctggcc ggactcctcg ccggcggcgg cctcgcccag
     1441 gcccagacgt cctattccag tgggtactgg tacgggaacg ccagtcccgt atcggtgctg
     1501 acccaccaca atgacggctg gcgcaccggc tggaattacg ccgagaccac cctcaccccg
     1561 tccaacgtgg cagccacggg gggcagcaag ctgttcggcc tgcagggcag cgtggatctg
     1621 gacggcctgg tggaggtgca gcccctggtc gtgcccaacg cctatgtgta cggcgatccg
     1681 aatgccggcc gccatgacgt ggtgtacgtg gccaccgcca acaacaccgt ctatgccatc
     1741 gacccgacca cccgcaaggt gctcaggagc cgcaacctgg gcacgcccat gtcggcgttc
     1801 cccggttgcg aggacaacga caagatcatc ggcgtgaaca gcacgccggt catcgacccg
     1861 tccaccaatt ccctgtacct gattgcccat gtcagggtcg gcagcggctc cgagatgcac
     1921 ctgcaccgcc tgtcgctgag ctccctcaag gacatcgtgg cgcctgtaac catcgcggcc
     1981 aaggcccccc tcaccaacgg cagcaccgcc accttcgacg ccaactatca gcgccagcgc
     2041 accgccctgc tgctggccaa tggccgcgta atggccgcct tcacctccca ctgtgacatc
     2101 cgcgccgacg tggcccgcgg ctgggtgatg cagtgggacg cccagaccct cgacaaggcc
     2161 cgcagctacg tggacttcag cggccgcaag tccgtgttcc tgaccgacac ccaggctacc
     2221 ggcccgagca acttcttcct ggcgaccagc tggatgtccg gtgccggccc ggccgtggat
     2281 gaatggggca acgtgtattt cgccaccggc aacaccgatc cgggcgccct gcgcagcgac
     2341 ggcaccggct acgtggagga tggcgtcacc aacttcgggc agagcgtggt caagctttct
     2401 ccgtgggaca gccgcgtgct gagctacttc accccggcca atgtgatgca gttggacaac
     2461 gatgacctgg acctgggcgc cggtggcgtc ctcctcgtgc ccagctacac cgaccgcctc
     2521 gccctgcagg ccggcaagga cggcgtgctg cgcctgatga accgggacag cctgggcggc
     2581 catacaccgg gcggaccgga caaggtgctc gccgccacgc agatcgggcc gtgctggtgc
     2641 accccgtcct acttcaacga cggcacgccc aaggtggtca gcagcggcgg taccacgctc
     2701 ggcgtgtatg ccatcaggac caagccctat ggcctcgtga aacaggccgg cgtggagatc
     2761 aattccggcc agttcggcgg ctttttcacc agtgtgtcgt ccaacggcgc ccagaacgcc
     2821 atcatctggg cggtgggccg accccagacc attccgggcg atctctatct gcacgcctat
     2881 tccgccaagc cggtgaacgg cagcctgccg ctgctgtaca ccaccaccgc cggcacctgg
     2941 aacagccagg gcaacgccaa caccgtgccc accgtggcca atggccgtgt gtatgtggcc
     3001 ggctacaaga aactgtccat ctacggcctg ggtggccagc gctgatcgcg aggaagattc
     3061 catgaagacc acacgcatcc tgatcctcgc cgccgccctg ggcggtgtca ccgccccggt
     3121 cctcgccgag gcgcccaatg ccgtcactgg cgtggtccgc agcattgccg gcgccaccct
     3181 gcaggtggaa ctgcgcggcg gcacggccct gcaggtcgag cagagcgacg ccctggcggc
     3241 ccaccaggtc gcgccgggca tcaaggttgg caccgccgtg catgcgacgg gtacccgcag
     3301 cggcaacctg ctgcgcgccg agaccgtgac cagggccaag ccggcgccag ggacctggtc
     3361 gccggatcgc tgattccccc ggtccggacc gcggcgcacg gtccggatcc ggcacctgcg
     3421 caccagcaag gccaacgacc cccgacggcg atcactcccg tgccgtcggc gggctcgggg
     3481 gttggccgac gtccccgcaa tccccgcctg ggcgatgtcc tggcggggat tggcgtttac
     3541 cggcagcggg ctggagcgga ccgaccgggc atcagacagg taaggtggcg tccgccgggc
     3601 ggggctgcag gatcgcgcgg gcctggagca ggccgtccac gccgcgggac gggcggcgca
     3661 gggggtcggc cgccgggcgg ccgagcaggt ggccctgggc acagtcgcag cccagatcga
     3721 agagcgtggc cagctcgcgg gcgtcttcca cgccctccgc gaccacctgg acgccgcgcc
     3781 gggcagcctc ggcgagaacg ctggccagat cgaggcggcg caggggatcg gcgtggatgc
     3841 gatggaaggc ggcaggtccg agcttgagga aggctggccg gaatcccgcc cgctcctgag
     3901 gtggcagcgc gccgtgcacc gcgatgcgca cgccctgggc gtccagggac gcgcaggccg
     3961 tcgccgcaca cccggttgca ggtgcatcgg gcagaggctc gacgaccagc cgggtgggcg
     4021 gcaggccggc ccggccggcg agggccacca cgccgtcggt gcgagcctgg tcgatgaggg
     4081 ccgcaaggct caggttgagg aacaggctgc cgggcagctc cacggcggca aaggcttcca
     4141 gcgcgaggcg gctggcgagg cgctccaggg ccagctccag gccggcttcc cgtgccacct
     4201 ggaggagctg ggccggggtg ttgagcggcg aatcgtcggg gccccgcatc agggcttcgt
     4261 agccggtgat ctcccggcgg cgcagatcga agatcggctg gaagacgaag tccagcgccg
     4321 agcaggcaat gagggtctgg agatggctga cgagatcgct gacgtacatg gtgaagcagg
     4381 gcgcgggaaa tggcggggca ataccgtcat tctcgacaga ctttcatccg gattgaatga
     4441 attttttctg acggctgcca gcgttgcggc cgttggcacc ggatccggcg ggcccctgcg
     4501 tcaggcgggt gggtgcaggc cgcggaacag gtcggcgtcg gcggcaaagc gttcggccag
     4561 ccaggtgctg accgcgtcga cgcgttccag gcggcgggca tcgcgatgga cgagctgcca
     4621 cagttcccgc accgggagcg cgtccgtggg cacgcgccgc agatcgctac aggcatcgcc
     4681 gatgaagcac ggcaggacgc cacagccgat gccggcggcg atggcgcggg tcaggccgcg
     4741 caggctgtcg cagcgcaggc ggatgcgggc cgcggtgttg cccgccgggc cgttcaggcg
     4801 ggccgcgagc cagcgcatct cgggcgtgtg gtcgaggtcc tcgccgtagg ccacccacgc
     4861 gacggggtcg atgggcgtgt cggcaggcgc atagaccgcg aaccccacgt ccgccagctt
     4921 gcggatcagg aaatccccgt ccgccggtcg ccccaggcgc agggcgatgt cggcctcgcg
     4981 acgggccagg ctgaggttgt cgttgcgggc gatgaggtcc acgtcgagcg ccgggtaggt
     5041 gctcatcagg ctcgccaggc gcggcgcgag gtagtcggcg acgatggggc ccaccgaggt
     5101 gaggtgcacc cggccgctca cgctgcgggc gccggcatcg gcgatgcggg tgatgtcggc
     5161 ggcgtcctct tccatgcgcc aggcccgcgc caggacctcg ctgcccgggg cggtgggctg
     5221 ccagccgccc tcgccccgtt cgaacagggg ggtaccgagg gcgtcctcga gggcccgcag
     5281 gcggcgggcg acggtggtct ggtcgatgcc gagacggcgc gccgcggcgg ccagggtgcc
     5341 cgcccggccg atggccgcgg cgatgcgcag atcgtcccag ttcatggtgg gggctccggg
     5401 atacaaagct gcattattgc aggcctgatc tgcaaggatg tcgattgttg caggtaatac
     5461 ggggcgctac gctggcgccg tcttcacatc ctgcaaggag cttgcgatgg ccgatgcctt
     5521 cacgttacgt gccctgatgg gcaaacccgt cacgccgacc cgcctgtccg aatcggtgct
     5581 gatcctgatc gatgcccaga acgcctaccg ccatggaccg ctgaccctca gcggcatcga
     5641 gccggccctc gcgtcctgtg cccgcctgct ggcccgcgcg cgggcgctgg gcgcaccggt
     5701 gatccacatc cagcatgacg ccggtccggg cacccctttc gatccgctga gcgaaagcgg
     5761 cgcctttatc gacgtcgtcg cgccgcgacc cggcgaggct gtcgtcgtga agcactttcc
     5821 caatgccttt gtgggcaccg acctggaggc gcgccttcag gcgctgggcg cccggaatct
     5881 ggtcatcgcg ggcttcatga cccacaactg cgtcaattcc accgcccgcg gcgcctacaa
     5941 cctgggctat cgcttcacgg tgccgggcaa cgcgacggcg acccgcgcgc tgccggcgat
     6001 cggcggtgga caactggacg cggcgaccct gcaggccgcc ttgctggtgt ccttgacgga
     6061 cgtgttcggc gtggtggtgg ccgacgaggc cgagattccc gactgaggag tcccggggcg
     6121 cccggctcgc ccttgcgggg ggacggttcg ggcgccgacg ccacgctcag aggatctcgg
     6181 tgagggtgtc gcgctgctcc atttcgtggc cgaactggat catctccagc ccgagggcgt
     6241 cgcgcaccga gacgatgccg atgggccgcc cggcctcgtc caccaccggc aggtggcgga
     6301 agcccccgtc gaacatgatg tggagcgcgt ggcagaaggg cttgtctgcc gggatggtct
     6361 ggggattcgg cgtcatgatc tcggccagcg gcgtgacggc ggggtcgcgc cccgcggcca
     6421 tgaccttctg gaccaggtcg cgttcggagc agatgccctc gacgcgcccg gtggcgttga
     6481 cgacggtgac cgtgctcttg tgctgatccc gcatcatgcg ggcggcctcg gcggcagagg
     6541 tggtgctggg ggccttgaga aagctgcggc ggctcacgac ctgggatacg ggacgagtcg
     6601 gcatgggagt ctccgggggt gtgggaggga ttcgtttgtg atgcgcgccg ggttgcgggt
     6661 tccattggat gttgcaatga tttccgcggc ttccggtggc gcattcaatt ggacaggccg
     6721 gcggtcggca ccatgggcgg gcggggggct atcatgtcgc gctgtttctc cggagaagtg
     6781 tcatgccagt ctggcccgcg ggcgtcggcc cgtgtcgcag gagccgtcca tgacggcgcc
     6841 gcccgtcgcc gcatcggccc ggcgcgtgtt gtccctcatc ccgccgatga cccagctcaa
     6901 cacgccctac ccctccacgg cctacctcac cggcttcctg cgcagccgcg gcgtggacgc
     6961 ggtgcaggac gacctggccc tcaagctggt gctggagctg ttctcgccag acgggctgga
     7021 ggcgatccgg gcccgcgccg aggccttgcc ggcgcggcgg cagtcgcccg cggtgcaggg
     7081 cttcctggcg cagttcgcgc gctaccgtgc caccgtcggc ccggcggtgg cctatctgca
     7141 gggacgcgat ccgactgttg cccatcgcat tgccagccgc cagttcctgc cggagggcgg
     7201 gcgttttgcg gccctggacc agtacgtgga cccggacggc ggcgatccgc tggcctgggc
     7261 gttcggtgcc ctgggcacgg cggacaaggc acgccatctg gcgaccttgt acctcaacga
     7321 tctggcggat gtgctgcgcg atgcggtgga cccgcgcttc gagttcgtcc gctacgccga
     7381 atccctggcc cgcagccagc ccggcttcga gcccctggcg cgggcccttg cggcgccgcc
     7441 gacgctggtg gacgaggtgc tggaaagcct cgccgcggag gccctgaccc gtcatgcgcc
     7501 ccaggtggtg ctgctgtccg tgccctttcc gggctcggtc tacggcgcct tccgcatcgc
     7561 ccaggcgatc aaggcccgcg ccccgcaggt ggtgacggtg ctgggcggcg gctttgccaa
     7621 caccgaactg cgcgagctgt ccgaaccgcg ggtcttcgac ttcttcgact atgtgacgct
     7681 ggacgacggc gagcgacccc tcctcgccct gctggaacac ctggacggcc gtcgcgggcc
     7741 gtcgcgcctg gtccgcacct accgccgcga cgaggccggc atggtgcgtt acgagcactg
     7801 ggtggagccg gacgtgccct ttgccgaggt gggcaccccc acctgggacg gcctgcccct
     7861 ggaccgctac ctgtcggtgc tggacatgct caacccgatg caccgcctgt ggtccgacgg
     7921 gcgctggaac aagctcaccg tggcccacgg ctgttactgg aagaagtgca gcttctgtga
     7981 cgtgagcctg gactacatcg ggcgctacga cggcgccgcg gccgcgctgc tggccgaccg
     8041 catcgacgcc atcgtcaccg agaccggtga gagcggcttc catttcgtgg acgaggcggc
     8101 gccgcccaag gccttgcggg ccctggccga cgagctgctg cagcgcggtc gggcgatctc
     8161 ctggtggggc aacatccgct tcgagaagtc cttcaccccg gaactgtgcg aacgccttgc
     8221 cgagagcggc tgcatcgccg tgtccggcgg cctggaggtg gcctcggacc gcctcctcaa
     8281 gctgatgaag aagggtgtgt cggtggatca ggtggcgcgg gtgacgcggg ccttcagcga
     8341 cgcgggcatc ctggtgcacg cctacctgat gtacggcttc cccacccaga ccgtgcagga
     8401 cacggtggat gcgctggaat acgtgcgcca gctcttcgac gcgggctgca tccagtccgg
     8461 cttcttccac cgctttgcgt gcacggtgca ttcgccggtg gggctcaatc cggccgaata
     8521 cggcgtcacc ctcaaggcgc tgccgccggt cagcttcgcc aggaacgacg tggaattcgt
     8581 cgacccgagc ggcgtggatc acgacgccct cgggcgggcc ctcaacaagg cgctgtacaa
     8641 ctacatgcac gggatcggtc tggatgagga cgtgcgcgcc tggttcgagg gccgcgtgcc
     8701 gcgaacgagg gtgccacggc gcttcgtgga gcgcgccctg ggcgtggcgc cggcgcgggc
     8761 ctgaggggca tcagcgggcg agcagccagg cgtcgaattc cgcggcggga agcggctcgc
     8821 tgaacaggaa gccctgaagc agcgggcagc cgcggcgcgt caggaaatcc acctgggacg
     8881 ggctctccac cccttcggcg accacttcca tgcccagatc ctgggcgagg gacagggtgg
     8941 ccgaggcgat ggcggcgtct tccaggtcgg cgggcagggc ttccacgaag ctgcggtcca
     9001 gcttgaggcg gcggatgggc aggcgcttga ggtggctgag gctggaatag cccgtgccga
     9061 agtcgtccag ggccaggttc acgcccaggg cgcgcagggc gtgcatgcgc tccagcacct
     9121 ctggcgtgag ctgcatcagg gcgctctcgg tgagctccag ttccaggtgg gccgggtcaa
     9181 tgccggtgtc ttcgaggatg cgcttcaccg tgccgtaaaa atgggcctgg cggaactgca
     9241 gggcggagat attgaccgcc atgatcagcg gccggccggc cgtggcccag atcacctgct
     9301 ggcggcaggc atgttccagc acccaggcgc cgagggcatg gatgatgccg ctggcctcgg
     9361 cgacgccgat gaactgggcc ggcggcagca ggccgagctc cgggtgctgc cagcgcacca
     9421 gggcctcgca gccggtcacc ctgctgtcgc gggtacgcag ctggggctgg tagtgcaggc
     9481 gcagttcgtc ccgttcgacg gcgcggcgca gggcggtttc cagcagcagc cgctccatcg
     9541 cacgcacgtc catgtccggc aggaagtagt ggtaggcgtt gcgtccctgg tccttggccc
     9601 ggtacatggc cagatcggcg tgcttgagga gggtgtccac gtcgctgccg tcgtccgggt
     9661 gcagggcgat gccgatgctc accgacacgg tgagttcgtg ggcgcccatg accaccgggt
     9721 gttccagcgc ggcaatcagc ttgcgcgcca ccagggcggc gtcttcccgg tgggacaggc
     9781 gcggcagcag gacgacgaac tcatcgccgc cgaggcgggc gaggaagtcg ttctcccgca
     9841 ggcagccgct caggcggcgg gccaccgcgg agaggagctg gtcgcccacc ggatggccca
     9901 gggagtcgtt gaccgccttg aagtggtcca ggtcgaggaa cagcacggcg aggatgtcac
     9961 cgtggcgggc ggcggcgcgc agttcggagt gggcccgctc gttccactgg gtgcggttgg
    10021 gcaggccggt gagcacgtcg aaatgggcca gccgggcgat gcgggcttcg gcggcgcggg
    10081 cggatgtgac gtcctgggcg gtcccgcgga tgcgcagcgc ggggccgtgg ccctcgctct
    10141 cgaggcggaa gtggaggatc tccatggggg tgccgtcccg ggcgaggcgg cagtccaggt
    10201 cgatgcggtg cggcatcgcg tccacctgct cgagggcgtg caggaagcgc gggcggtcgt
    10261 ccggatgcag gcgctcgatg agctggcggg tgcgtatcgg cgtgctcccg tcaagggtga
    10321 gcatggccat cagttcgtcg gagcagcgca gttcgtcgct gcccggcagg gtttcccagc
    10381 tgcccagacg ggcgatgcgc tgggcctggg cgagttcctg ctgctggcgg cgcagggtgt
    10441 cggtggcggc agcaagctgg cgggtgcgct cgtccaccag gctctccacc cggcgggtgc
    10501 ggccgctggt gaggagcagg aaggcgccga ggatgccggt gctgagcagg cccagggcca
    10561 gcatcagcca cagaccccag ttgcccacgg tgttcatgta ggcggaggtg gcgccgacgc
    10621 gcagcagcca ggtccgcccc gcaaaggtga ggggggtttc ccagttcagg cgcttgccgg
    10681 cccagcccgc ctgctcgcac cccgccgggc cgtagaggcg ctcgctggcg ccggtccggg
    10741 gttcgctcag gcagacgtcg agctccccgg tccggcggtg cccgaggatg gcctgcatgg
    10801 cgtcgtccat gcggaacacc gccgacacca ggccggtcat gtgcggcggc gagccggcca
    10861 gcaccgcctg atacacgacg atgccgcgct gctcgccggt ttcctggacg aggcggatgg
    10921 attcgctggc gatgggcagg ccggacttgc ggctgcgctg cacggcgctg gcggtcctgt
    10981 ccagcacgag gggatcgagc ccgagcacgc tgcgattttc ggcgaggggc tcgacgaaca
    11041 ggatgggcag gtattcggcc ttgcgcgcgg ccggaaaggt ctgcccggca acgttgcggc
    11101 caaggatgcg gaaatccggc cgcccggccg cccgcacgcg ggcttcgaag gcgtcccgct
    11161 gcgcgtcggt gacgagggga ctccagccga agttctgggt gcccggattg cgggccatca
    11221 tcggcgtgac gaaatcgcgc ca
//
LOCUS       DAOQMM010000119        11114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2725_length_11114_cov_43.664075, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000119 DAOQMM010000000
VERSION     DAOQMM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2725_length_11114_cov_43.664075"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..712
                     /locus_tag="PLH85_15070"
     CDS             65..712
                     /locus_tag="PLH85_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65938.1"
                     /translation="MPAPSPSQLDSPTSGFLKGAGLNGEDVPGLATAIANTAGQALTL
                     FLAQALVMPGIPVAADPISGSGASAGPGRLMPPPAGGPGASQLEGLATAQCQAQGLRG
                     EDADGLAKAIAGTVAQGIALFCAQSMVLPGIAVAGFVSAAPGLLQPVPLASSLEGIAN
                     GLLAQNGLRGEAAPDLAKALAQGVDLALTQFAAQAMVSPGIPCPPGASAGPGRLM"
     gene            730..1143
                     /locus_tag="PLH85_15075"
     CDS             730..1143
                     /locus_tag="PLH85_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65939.1"
                     /translation="MATDPFGTDLRLVFTRGREGADLAAGFSDLESVSGLENLVQALT
                     LRLLVDRGELAGLGHPRYGSRIRDLIGEPLDRPNLELLRRYVRQELLRDPRVAEVVQV
                     AVTPHDERRDGVDVVARVRATDGREARVEVSLDGA"
     gene            1133..2650
                     /locus_tag="PLH85_15080"
     CDS             1133..2650
                     /locus_tag="PLH85_15080"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNB65940.1"
                     /translation="MALERVFRKKDFAEVVDALLGDIASGLGGRVALTDAAEGSVVRT
                     LAEAFARELAVCYEQLDSVYRNAYLDSASGAALDNVVALLGLERQRGGHLEGSVVFGR
                     GQPAPEDIHIPLGTLVAGRGVPLFATTAGSVLDKGERSVLVGVRAVDPGGATVKAGLL
                     THLPRPIAGLESVTNPGDLLLRQREETDEELRARARRLVLSSHTGTVAALEEAVRSLG
                     IREVQVIENPGGRPGEVEVVLGDTDIPADLLEQVRNRLEDVRPAGIVLRCGPATPVFV
                     QVTATLTLNADLPEREQRDIEKRIGDALIAYFGQLKVGEAVRAAKLRALLLGHDAIVG
                     TEPTGQSPLMEPFVWNDGALQSVAFRSLLGNGDVLAGPGERIGLYPEALPLRLSLEPP
                     LLPVQVDADLTLAAGSAVAGLDARVTAVLKDTFERAARRATTGTPADIPYAELRDAVA
                     AALPAAKILRLRVSVIHERDGRVEELDDAAGHATLATREQAMMRRIAVRAEDGHG"
     gene            2643..4496
                     /locus_tag="PLH85_15085"
     CDS             2643..4496
                     /locus_tag="PLH85_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65941.1"
                     /translation="MADPRQRRERLLALLPRIYTAQPRDSAVGAVVDALAAALARVDD
                     SLTRVQYDRWVGLASPLREGDDISALEALGRLLQVNRLPPRLAHAGYDPDGPTRLSVR
                     FDAHAPLADALHDLIGDAWRQPDENGRPGDPAALLAEQFPGVRFALDDAARLQADADT
                     PAALARFRALLDPEPGEIFRQRLRVTAQVRTGGLTTPRALLALALADLGAEPCPRLER
                     RQDSTLARGMAPGTRKRCAACAGDGPCPNTPAAVVEAWLTEHPVEARRHTEPAPRLRR
                     VFTLDNPSLLPDRPVWRLTVSDQPAAYPAVRSLESGEITLFAGTLKPGDTLVVQPALS
                     PTERAGFDGYDSPGHHAWSASHPAGRAIVIDADGAERDVSDAVFYLWGTRFDAPEATL
                     DGGMRCGVLDQRVVTPQLRPGSNAWMLLTFAKPDAEFADAGSTVHTSRFAGPQDADGT
                     RFALLDGSLARSDAKFATLLFEAISKTDTASTTEGSTANAARLRLDLEWVARPPATAR
                     LRIPKNEWVAAAAARGVLALVRSDVTGASAAGVRIQVDFPEPVRREYVVSGEGLSLHT
                     GQRWREDAAPSERGPHIGLKLTLRDSAAPQEGHLSTGLVFDTTRLDWSHAG"
     gene            4522..4998
                     /locus_tag="PLH85_15090"
     CDS             4522..4998
                     /locus_tag="PLH85_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65942.1"
                     /translation="MSLKDCHGLAGRLHIELRDPRGTLVERRTVNNLITTSGRRLLAS
                     LFAGAAQGPGLQIAVGGSGGEVLPGDTQLGSQVDVAAATIPAIGEDTVDGAPRVVLRV
                     VATLPPTGKPDAQALQEAGILVTVSGAEPVLYNRVTFPLINRAGNLEMTLTWEVIF"
     gene            4998..6398
                     /locus_tag="PLH85_15095"
     CDS             4998..6398
                     /locus_tag="PLH85_15095"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNB65943.1"
                     /translation="MADTPTPYVRKDPGDIMLAADWNQMQVRARQEIQAHDHSGDPFQ
                     QIPREGIREKAIDGSRIDPDAKVALKGLTVSGPLKITGTSQLADIKAALASFTGARLT
                     TAGDLGVGTDAPENAEGWSRVVDILGGAHAKLAVRAGDVDARVMAHTAGIYGGEPGML
                     VGTRSGHPLSLVQAGTVRARLATATAQGGSETGLLDLFPAGSPLRISHGWTSFPDANA
                     LAAEICNDTQIYKTLMLVGNRSNGGVRRVSVWDRLEVNGPLTAQGVDVSAGVPDHIST
                     DGSFYRWGGQVYLNVDDNLYVRDSQRGIRMHFDTTNGVFKTDVLRLGDKWRMSAIGDA
                     HGNDGWLRLFNVQNTGYFGGLAAGELWSGKALQVSDRRLKDNVREIGDALDKLSRLHG
                     VHFDWREGAPSGVGRPAAGLIAQDVEGVLPEAVALGPDGWKGIDTTAVLALLVQAVKE
                     QQVLIQRLRDELATRA"
     gene            6412..7812
                     /locus_tag="PLH85_15100"
     CDS             6412..7812
                     /locus_tag="PLH85_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65944.1"
                     /translation="MPTRPDPYVKRAAGDIIRADDWNEAQVRAAEALAAHDHRAGQGQ
                     PVPREGIAEKAIDGSRIDPAASVTLQALDVRGELKVNARALLAEIDGLIAGLKNALNR
                     NGDVLRGSLAITERLAVGSEKLASARLQLGELSALDIGARNNAWTNLGWNAYFDGAWK
                     RLDPNKPGANLHLSGDGAGQEFRFLRVEADGSALRNVAVIGSKVSAILESNLGLGTAA
                     PNARLDVRGAIHAGGSDLYFTETGHRHTGFGNAAGYAAIENDGGDYNALMILGRSISQ
                     NPLSRAVKMYDNVTISGNLGTHGFAPSPRTGGWGGGIHTWDVEAEGTMWSRNGYQSGN
                     RDLAENFDAVEALAPGDVVSLDPDRDGIRRSRQARDPLVLGVVSTRPGVLLGADADRQ
                     PPFAGEVPVALSGRVPCQVTDENGPIRRGDLLTAASRPGHAMRAGDAPPGTLIGKALA
                     DFDGGTGTIDVFVFVR"
     gene            7835..8032
                     /locus_tag="PLH85_15105"
     CDS             7835..8032
                     /locus_tag="PLH85_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65945.1"
                     /translation="MKNELTARLAQLHEEYDAGTRVMADLQKKQDELRTTLLRISGAI
                     QVLEELLGESAEQPAKPPPAA"
     gene            8040..9335
                     /locus_tag="PLH85_15110"
     CDS             8040..9335
                     /locus_tag="PLH85_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65946.1"
                     /translation="MADPVQLLGPDGAPAAGRSFSAAEGIRLRVTLPAGRRLAEARLS
                     LASADAAEQAVLYPPLQTDAGAADGQTEASWRWAAIDWRHERALTGVIIDARDASTPP
                     RTCAARVKLYSRGHWLPLTPLDALPTGAVQRFPPLAASRLMAEMLVEGDVGGSRSGVP
                     IPGALTGKRIGAILTGQACHVSLAMEDLPPFFAPEGPLPTQAQPVEGLTRILNRYLSD
                     HPGTTEIPLLLRAAGDEAVRVGPFAATLEAPAQADDAAAPPVDPHQARPGEQPWFPPG
                     RAPLQAARLIDPQHQAAQSFAPLPADRSLSSTALRLRPLADAFVGNVRLHALRGDQTV
                     EPPLAEWPLSFGPDSAQSADAVWLRFRLPETVAPAGPWRLTCQATSGALLWYVDDSPR
                     PAPLQSGLARTGQGPWLPLDGPGEAPWLQVEVGLLDTAP"
     gene            9345..10085
                     /locus_tag="PLH85_15115"
     CDS             9345..10085
                     /locus_tag="PLH85_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65947.1"
                     /translation="MLAAIEALFLPALRAALGPDASLRSGPAGAPAGPSGQVSLWAEK
                     LEWDGAEGEDPTRDPVCAGWQGEVSPDPASPLDFPLPEAARGEVAEVQSPPGRILPPG
                     DAWQIDGRTLRFFSAPGGPVRVSTRTVPVRGYRERRFGHVALSLRAWAHDGPAVDSLL
                     ATGLAALLAAAENFNVVELAGAAPSLSVRLTRPRLSVVALEQDADGPWTSGRVRCVLR
                     GEVELALRLGEAAPEGRIRDVTVTLRRP"
     gene            complement(10110..>11114)
                     /gene="wecB"
                     /locus_tag="PLH85_15120"
     CDS             complement(10110..>11114)
                     /gene="wecB"
                     /locus_tag="PLH85_15120"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB65948.1"
                     /translation="FKIIHTGQHYDREMSDVFFDELGIPKPDYHLEAGGGSHATQTAK
                     IMVAFEEICQKVRPDCVLVVGDVNSTLACAIDAKKLQIPVAHVEAGLRSGDMRMPEEI
                     NRLVTDSISDWFFCTEPAGVDNLLKEGKQPEALFHVGHVMVDNLLYQRDQLDASDKSG
                     MESEALKRRYPKYGVVTLHRPSNVDDETHLRGIAGALRQIAADLPLIFPVHPRTRGNL
                     EKFGLDLGPNVVLTKPQSYMEFLNLWKDAALIMTDSGGLQEETTALGIPCLTLRENTE
                     RPVTIDEGSNVLVGPDPARILEEAAKVLAGQGKQGRRPALWDGKAAERIVADLDRLLP
                     "
BASE COUNT         1599 a         4341 c         3697 g         1477 t
ORIGIN      
        1 tactttaccg gcaaggccct ggtgccctcc ggcaccgtca aggccaaagg ctaggatccc
       61 gcccatgccc gcccccagcc cctcccagct cgattcgccg accagcggct tcctcaaggg
      121 cgccggcctc aacggcgagg atgtcccggg cctcgccacg gccatcgcca acaccgccgg
      181 ccaggccctg accctcttcc tcgcccaggc cctggtgatg cccggcatcc ccgtggcggc
      241 cgacccgatc agcggcagcg gcgccagcgc cggtcccggc cgtctgatgc cgccgccggc
      301 aggtggcccc ggcgcgtccc aactcgaagg cctcgccacc gcccagtgcc aggcccaagg
      361 cctgcggggc gaggacgccg acggcctcgc caaggccatc gccggcaccg tcgcccaggg
      421 catcgcgctg ttctgcgccc agtccatggt gctgccgggc atcgccgtgg ccggcttcgt
      481 cagcgccgcg ccgggcctgc tccagccggt gcccctggcg tcgtcgctgg aaggcatcgc
      541 caacggcctc ctcgcccaga acggcctgcg tggcgaggcg gcgccggatc tcgccaaggc
      601 tctcgcccag ggcgtggatc tggccctcac ccagtttgcc gcccaggcga tggtgtcgcc
      661 cggcattccc tgcccgcccg gcgccagcgc cggcccgggc cggctcatgt aagcccacgg
      721 accgcgccca tggccaccga ccccttcggc accgatctgc gcctggtctt cacccgcggc
      781 cgggaaggcg ccgacctggc ggccggcttc agcgatctgg agagcgtcag cggcctggag
      841 aatctcgtcc aggccctcac cctgcgcctg ctggtggatc ggggcgaact cgccggcctg
      901 ggccaccccc gctatggcag ccgcatccgc gacctgatcg gcgagcccct cgaccgcccc
      961 aacctggaac tcctgcgccg ctacgtgcgc caggagctgt tgcgcgaccc gcgcgtcgcc
     1021 gaggtcgtgc aggtggcggt cactccccac gacgaacgcc gcgacggcgt ggacgtggtg
     1081 gcccgcgtgc gcgccaccga cggccgcgaa gcccgcgtgg aggtgagcct cgatggcgct
     1141 tgaacgggtc ttccgcaaga aggatttcgc cgaggtggtg gacgccctgc tgggcgacat
     1201 cgcttccggc ctgggtggcc gtgtggcgct caccgacgcg gccgagggca gcgtggtgcg
     1261 caccctcgcc gaagccttcg cgcgggagct ggcggtgtgc tacgagcagc tcgacagcgt
     1321 ctatcgcaat gcctacctgg acagcgccag cggcgccgcc ctcgacaacg tggtggccct
     1381 gctcggcctg gaacgccagc gcggcggaca cctggaaggc agtgtcgtgt tcggccgcgg
     1441 ccaacctgcg ccggaggaca tccacattcc cctcggcacc ctggtcgccg gccgcggcgt
     1501 gcccctcttc gccaccacgg cgggcagcgt gctcgacaag ggcgagcgca gcgtgctggt
     1561 gggcgtgcgt gccgtcgatc cgggcggcgc caccgtcaag gccggcctgc tcacccatct
     1621 cccgcgcccc atcgccggtc tggagagcgt caccaacccc ggcgacctgc tcctgcgcca
     1681 gcgcgaggag accgacgagg agctgcgcgc ccgcgcccgc cgtctcgtcc tgtcctccca
     1741 tacgggcacc gtcgccgcgc tggaggaagc cgtccgcagc ctgggtatcc gcgaagtgca
     1801 ggtgatcgaa aaccccggcg gccgtcccgg cgaggtggag gtggtgctgg gcgacaccga
     1861 catccccgcc gacctgctgg agcaggtgcg caatcgcctg gaggacgtcc gcccggccgg
     1921 catcgtgctg cgctgcggcc ccgccacgcc ggtcttcgtg caggtcaccg cgaccctcac
     1981 cctcaacgcc gacctccccg agcgggaaca gcgcgacatc gaaaagcgca tcggtgacgc
     2041 cctgatcgcc tatttcggcc agctcaaggt gggcgaggcg gtgcgcgcag ccaagctgcg
     2101 cgccctgctc ctcggccacg acgccatcgt cgggaccgag cccaccggcc agagcccact
     2161 gatggagcct ttcgtgtgga acgacggcgc gctccagtcc gtggccttcc gcagcctgct
     2221 cggcaacggc gacgtgctgg ccgggccggg cgagcgcatc gggctgtatc cagaagccct
     2281 gcccctgcgc ctcagcctgg aaccgcccct gctgccggtg caggtggacg cggatctcac
     2341 gctggctgcc ggcagcgccg tggccggcct cgatgcccgc gtcacggccg tgctcaagga
     2401 caccttcgag cgggccgcgc gccgggccac caccgggaca ccggccgaca ttccctacgc
     2461 cgagctgcgc gacgccgtgg ccgccgccct gccggcagcc aagatcctgc gcctgcgggt
     2521 gagcgtcatc cacgagcgcg acggacgggt cgaggaactg gacgacgcgg ccggccacgc
     2581 caccctcgcc acccgcgagc aggccatgat gcggcgcatc gccgtgcgtg ccgaggacgg
     2641 ccatggctga tccgcgccaa cgccgggagc gcctcctcgc cctgctgcca cgcatctaca
     2701 ccgcccagcc gcgggacagc gccgtgggcg ccgtggtgga cgccctcgcc gccgccctgg
     2761 cgcgggtgga cgacagcctc acccgcgtcc agtacgaccg ctgggtcggc ctcgccagcc
     2821 ccctgcgcga gggcgacgac atcagcgccc tggaagccct cggccgtctg ctacaggtca
     2881 atcgcctgcc gcctcgcctc gcccacgccg gctacgaccc ggatggcccg acccgcctga
     2941 gcgtgcgctt cgacgcccac gcccccctcg ccgacgccct ccacgacctg atcggcgacg
     3001 cctggcgcca gcccgacgag aacggccgcc ccggcgaccc cgcggcgctg ctcgccgagc
     3061 agttcccggg cgtgcgcttc gccctggacg acgccgcccg cctccaggcc gacgccgaca
     3121 cgcccgccgc cctcgcccgc ttccgcgccc tgctcgaccc ggagcccggc gagatcttcc
     3181 gccagcgcct gcgcgtcacc gcccaggtgc gcaccggcgg cctcacgacc ccgcgggccc
     3241 tgctcgcact ggccctcgcc gacctgggcg cggagccctg cccccgcctc gaacgacgcc
     3301 aggacagtac cctcgcccgc ggcatggcgc cgggcacccg caaacgctgc gccgcctgcg
     3361 ccggcgacgg cccgtgcccc aacaccccgg ccgccgtggt cgaggcctgg ctcaccgaac
     3421 atcccgtcga agcccgccgc cacaccgaac cggcgccgcg cctgcgccgc gtattcaccc
     3481 tcgacaaccc cagcctgctg cccgaccggc cggtctggcg cctgaccgtc agcgaccagc
     3541 ctgcggccta ccccgccgtg cgcagcctgg aatccggcga gatcaccctc ttcgccggca
     3601 ccctcaaacc cggcgacacc ctcgtcgttc agcccgccct gagccccacc gaacgggccg
     3661 gcttcgacgg ctatgactca ccggggcacc acgcctggag cgcatcccac cctgcgggcc
     3721 gggcgatcgt catcgacgcc gacggcgccg agcgggacgt cagcgacgcc gtcttctacc
     3781 tgtggggcac ccgcttcgac gcccccgagg cgaccctcga cggcgggatg cgctgcggcg
     3841 tgctcgacca gcgcgtcgtc acgccccagc tgcggccagg cagcaacgcc tggatgctcc
     3901 tcacctttgc caagcccgac gcggaatttg ccgatgccgg cagcaccgtg cacacctcgc
     3961 gtttcgccgg cccgcaggac gccgacggca cccgtttcgc cctcctcgac ggcagtcttg
     4021 cgcgcagcga tgccaagttc gccaccctgc tcttcgaagc catcagcaag accgacaccg
     4081 cctccaccac cgaaggcagc accgccaatg ccgcgcgcct gaggctcgat ctggaatggg
     4141 tcgcccggcc gcccgccacg gcccgcctgc gcatccccaa gaacgaatgg gtcgccgccg
     4201 ccgccgcccg cggcgtgctg gccctggtgc gcagtgacgt caccggcgcc agcgcggcgg
     4261 gcgttcgcat ccaggtggac tttcccgagc cggtacgccg cgagtacgtc gtctccggcg
     4321 aggggctgtc cctgcacacc ggccagcgct ggcgcgagga cgccgcccca tccgagcgcg
     4381 gcccccatat cgggctgaaa ctcaccctgc gggacagcgc cgcaccgcag gaaggacatc
     4441 tctcgacggg cctggtgttc gacaccaccc gtctcgactg gagccacgcg ggctgacccg
     4501 cccgcccgag gagcacaccc catgtcactc aaggactgcc acggcctggc cggacgcctg
     4561 cacatcgaac tgcgcgaccc acgcgggacg ctggtcgaac gccgcaccgt gaacaacctc
     4621 atcaccacgt ccggccggcg cctgctggcc agcctgtttg ccggcgcagc ccagggccct
     4681 ggcctgcaga tcgcggtggg cggcagcggc ggcgaggtgc tccccggcga cacgcagctg
     4741 ggcagccagg tcgacgtggc cgcggcaacg atccccgcga tcggcgaaga caccgtggac
     4801 ggcgcgccgc gggtcgtgct gcgggtcgtc gccaccctgc cgccgaccgg caagcccgac
     4861 gcccaggcgc tgcaggaagc cggcatcctg gtcaccgtct ccggcgccga gccggtgctc
     4921 tacaaccgcg tcacctttcc gctcatcaac cgggcgggca acctggagat gacgctcacc
     4981 tgggaggtga tcttctgatg gccgataccc ccacccccta cgtccgcaag gaccccggcg
     5041 acatcatgct cgccgccgac tggaaccaga tgcaggtccg cgcccgccag gagatccagg
     5101 cccacgacca cagcggcgac cccttccagc agatcccgcg ggaaggcatc cgcgaaaagg
     5161 ccatcgacgg cagccgcatc gacccggacg ccaaggtcgc cctcaagggc ctcaccgtct
     5221 ccggccccct caagatcacc ggcaccagcc agcttgccga catcaaggcg gcgctggcca
     5281 gcttcaccgg cgcccgcctc accacggccg gcgacctggg cgtgggcacc gacgcgccgg
     5341 agaacgccga gggctggagc cgcgtggtgg atatcctggg cggcgcccac gccaagctgg
     5401 cagtgcgcgc cggcgacgtg gacgcccggg tgatggccca caccgcgggc atctacgggg
     5461 gcgaacccgg catgctcgtc ggcacccgca gcggtcatcc cctgagcctc gtgcaggccg
     5521 gcaccgtgcg cgcccgcctg gccaccgcca ccgcccaggg cggcagcgag accggcctcc
     5581 tcgatctctt ccccgccggc agccccctgc gcatctccca cggctggacc agcttcccgg
     5641 atgccaatgc cctggccgcc gagatctgca acgacaccca gatctacaag accctcatgc
     5701 tggtgggcaa ccgcagcaac ggcggcgtac gccgcgtctc cgtgtgggac cggctggagg
     5761 tcaacggccc cctcaccgcc cagggcgtgg atgtctcggc cggcgtgccc gaccacatct
     5821 cgaccgacgg cagcttctac cgctggggcg ggcaggtgta tctcaacgtg gatgacaacc
     5881 tgtacgtgcg cgacagccag cgcggcatcc gcatgcactt cgacacaacc aacggcgtgt
     5941 tcaagaccga cgtgctgcgc ctgggcgaca agtggcgcat gtcggccatc ggcgacgccc
     6001 acggcaacga cggctggctg cgcctcttca acgtgcagaa caccggctac ttcggcggcc
     6061 tggcggcggg cgagctgtgg tcgggcaagg ccctgcaggt ctccgaccgg cgcctcaagg
     6121 acaatgtgcg cgagatcggc gatgccctcg acaagctgag ccgcctgcac ggcgtgcact
     6181 tcgactggcg cgagggggcc cccagcggcg tcgggcggcc ggcggccggg ctcatcgccc
     6241 aggacgtgga aggcgtgctg cccgaagccg tcgccctggg gccggacggc tggaagggca
     6301 tcgacaccac cgccgtgctc gccctgctgg tgcaagccgt caaggaacag caggtcctca
     6361 tccagcgcct gcgcgacgag ctcgcgaccc gcgcctgatc cggaagccgc catgcccacc
     6421 cgtcccgacc cctacgtcaa gcgcgctgcc ggcgacatca tccgcgccga cgactggaac
     6481 gaggctcagg tccgcgccgc cgaggccctc gccgcccacg accaccgtgc cggccagggt
     6541 cagccggtgc cccgcgaagg catcgccgaa aaggccatcg acggcagccg catcgacccc
     6601 gctgcgagcg tcacactgca ggccctcgac gtgcgcggcg agctcaaggt caatgcccgc
     6661 gccctgctgg ccgagatcga cggccttatc gccggcctga aaaacgcgct gaatcgcaat
     6721 ggcgacgtgc tgcgcggcag cctggccatc accgaacgcc tggcggtcgg cagcgagaag
     6781 cttgccagcg cgcgcctgca gcttggcgaa ctgagcgccc tcgacatcgg cgcccgcaac
     6841 aatgcctgga ccaacctggg ctggaacgcc tatttcgatg gcgcctggaa gcgcctcgac
     6901 ccgaacaagc caggggccaa cctgcacctc agcggtgacg gcgcgggcca ggaattccgc
     6961 ttcctgcgcg tcgaggcgga cggcagtgcg ctgcgcaacg tggcggtgat cggcagcaag
     7021 gtcagcgcga tcctcgaatc caacctgggc ctcggcaccg ccgcgcccaa cgcccgcctc
     7081 gatgtgcgcg gcgcgatcca cgccggcggc tcggacctgt atttcaccga gacgggccac
     7141 cgtcacacgg gcttcggcaa cgccgccggc tacgccgcca tcgaaaacga cggcggcgat
     7201 tacaacgccc tgatgatcct gggccgcagc atcagccaga accccctgtc gcgcgccgtc
     7261 aagatgtacg acaacgtcac catttcgggc aatctgggca cccatggctt cgcccccagt
     7321 ccgcggacgg gcggctgggg cggcggcatc catacctggg acgtggaggc cgaaggcacc
     7381 atgtggtcgc gcaacggcta ccagagcggc aaccgggatc tggccgagaa cttcgacgcc
     7441 gtcgaggccc tggcgcccgg cgacgtggtc agcctggacc cggaccggga cggcatccgg
     7501 cgcagtcgcc aggcccgcga tccgctggtg ctgggcgtgg tgtccacccg ccccggcgtc
     7561 ctcctgggcg ccgacgccga ccgccagccg ccctttgccg gcgaagtccc cgtggccctg
     7621 agcggccgcg tcccctgcca ggtcactgac gaaaacggcc ccatccggcg cggcgacctg
     7681 ctcaccgcag ccagccgccc cggccacgcc atgcgcgccg gagacgctcc gccgggcacc
     7741 ctgatcggca aggccctggc cgatttcgac ggcggcacgg gtaccatcga cgtcttcgtc
     7801 ttcgtccgct gaaccatcac ccaccgcccc gccgatgaaa aacgaactca ccgcccgcct
     7861 cgcccagttg cacgaggaat acgacgccgg aacccgcgtc atggccgacc tgcagaagaa
     7921 acaggacgaa ctgcgcacca ccctgctgcg catcagcggc gccatccagg tgctggagga
     7981 gctcctcggc gagtccgccg aacagcccgc caagccccca cccgccgcct gaccccagca
     8041 tggcggaccc ggtccagctc ctcggtccgg acggcgcccc cgccgccggt cgcagctttt
     8101 ccgcagcgga gggcatccgc ctgcgcgtca ccctgccggc cggccgccgc cttgcagagg
     8161 cccgcctcag tctggcatcg gccgacgccg ccgaacaggc cgtgctgtat ccgcccctcc
     8221 agaccgatgc cggcgcggcc gacggccaga ccgaggcctc gtggcgctgg gcggccatcg
     8281 actggcgcca cgagcgcgct ctgaccggcg tgatcatcga cgcccgcgac gcctccacgc
     8341 ccccgcgcac ctgcgccgcc cgcgtgaagc tctacagccg cggccactgg ctgcccctca
     8401 cgcccctcga cgcgctgccg accggcgccg tgcagcgctt tccgccgctg gccgcctcgc
     8461 gtctgatggc cgagatgctg gtcgaaggcg atgtgggcgg cagccggagc ggcgtcccca
     8521 tccccggtgc cctgacgggc aaacggatcg gcgcgatcct caccggccag gcctgccatg
     8581 tcagcctggc gatggaggac ctgccgcctt ttttcgcccc cgagggcccg ctgccaaccc
     8641 aggcccagcc ggtggagggc ctcacccgga tcctcaaccg ctacctgagc gatcatcccg
     8701 gcacgacgga gattccgttg ctgctgcggg ccgccggcga cgaggcggtg cgggtcggac
     8761 cgttcgccgc caccctggaa gcccccgcgc aagcagacga cgctgctgcg ccgccagtgg
     8821 acccgcatca ggcacgccct ggcgaacagc cctggtttcc gccgggtcgc gcccccctgc
     8881 aggccgctcg cctgatcgac ccgcagcacc aggcggccca gtcgtttgcc ccgctgccgg
     8941 cagaccgcag cctgtcgagc acggccctgc gcctgcgccc tctggccgac gccttcgtgg
     9001 gcaacgtgcg tctgcatgcc ttgcgcggcg atcaaacagt cgaacccccg ctggccgagt
     9061 ggccgctgag cttcggaccg gacagtgcgc agtcagccga cgccgtctgg ctgcgcttcc
     9121 ggcttcccga aaccgtggcc ccggcgggcc cctggcgcct cacctgccag gcgacgagcg
     9181 gggcgctgct gtggtacgtg gatgacagcc cgcgcccggc accgctccag agcggtctgg
     9241 cccgcaccgg tcagggtccc tggttgcccc tcgacggacc cggcgaggcg ccctggctgc
     9301 aggtggaggt cggcctgctc gacaccgcgc cctgaggaac ttgcatgctc gccgccattg
     9361 aagccctgtt cctgcctgcc ctgcgtgccg ccctcggccc tgacgccagc ctgcggagcg
     9421 gcccggccgg cgccccggcg ggaccgtcgg ggcaggtgag tctgtgggcg gaaaaactgg
     9481 aatgggacgg ggcggaaggc gaagacccga cgcgggatcc ggtgtgcgcc ggctggcagg
     9541 gcgaggtgtc gccggatccg gcctcaccgc tggattttcc gctgcccgag gcggcccgcg
     9601 gcgaggtcgc agaagtgcag agcccgccgg gccgcatcct gccgccgggt gacgcctggc
     9661 agatcgacgg gcgcaccctg cgcttcttca gcgcgcccgg tgggccggtg cgggtcagca
     9721 cccggacggt cccggtccgc ggctaccggg agcggcgctt cggccatgtg gcgctgtccc
     9781 tgcgtgcctg ggcacacgac ggtccggccg tcgacagcct gctggccacc ggcctggcgg
     9841 cactgctcgc ggcggcggaa aacttcaatg tggtggaact ggccggcgcg gcgccgagcc
     9901 tgagcgtccg cctgacgcgg ccgcgcctgt ccgtggtggc ccttgaacaa gacgcggacg
     9961 gcccatggac atcgggacgc gtccgctgcg tgctgcgggg tgaggtggaa ctggcgctgc
    10021 gtctgggcga ggcagcgccg gaagggcgca tccgcgatgt gacggtcacc ctgcgccggc
    10081 cttgatcgcc gcgacggcga ccgccgtcat cagggcagca gacgatccag atcggcgacg
    10141 atgcgctcgg cggccttgcc gtcccacagg gcggggcggc gcccctgctt gccctggccg
    10201 gccagcacct tggccgcttc ttcgagaatg cgcgccggat cggggccgac cagcacgttg
    10261 ctgccttcgt cgatggtgac cgggcgctcg gtgttctccc gcagcgtgag gcagggaatg
    10321 cccagcgcgg tggtttcttc ctgcaggccg ccgctgtcgg tcatgatcag cgcggcgtcc
    10381 ttccacagat tgaggaattc catgtaggac tggggtttgg tgaggaccac gttgggcccc
    10441 agatcgagtc cgaatttttc caggttgccg cgggtgcgcg gatgcaccgg aaagatcagc
    10501 ggcaggtcgg cggcgatctg cctgagcgcg ccggcgatgc cgcgcaggtg ggtctcgtcg
    10561 tccacgttgg agggacggtg cagggtcacc acgccgtact tgggataacg gcgcttgagg
    10621 gcctcactct ccatgccgga cttgtcggac gcatcgagct ggtcgcgctg gtagagcagg
    10681 ttgtccacca tcacgtggcc cacgtggaag agggcctccg gctgcttgcc ttccttgagc
    10741 aggttgtcca cgccggccgg ctcggtgcag aagaaccagt cggagatgct gtcggtcacg
    10801 aggcggttga tctcctccgg catgcgcatg tcgccgctgc gcaggccggc ttcgacgtgg
    10861 gccaccggga tctgcagctt cttggcgtcg atggcgcagg ccagggtgga gttcacgtca
    10921 cccaccacca gcacgcagtc ggggcgcacc ttctggcaga tctcctcgaa ggccaccatg
    10981 atcttggcgg tctgcgtggc gtggctgccg ccccccgcct ccaggtggta atccggcttg
    11041 gggataccca gctcgtcgaa gaagacgtcg ctcatctccc ggtcgtagtg ctggccggtg
    11101 tggatgatct tgaa
//
LOCUS       DAOQMM010000120        10837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_2827_length_10837_cov_45.858653, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000120 DAOQMM010000000
VERSION     DAOQMM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2827_length_10837_cov_45.858653"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLH85_15125"
     CDS             <1..644
                     /locus_tag="PLH85_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB65949.1"
                     /translation="AADGPADFLPGQYAMLDLPGVGASRAYSLSNTPNDHGEWHFQIR
                     RVAGGRGTAVLFDTLRPGQEVGLDGPYGVAYLRPDAPRDLVCVAGGSGLAPMVSIARG
                     AARAGLLKTRRLHFFYGARTPRDVCGQDFLAALSGFGERVTYTPVVSAPEGTDWDGAT
                     GFVHSHMAAALPGPLSRYEFYFAGPPPMTQALQELLMVDHRVPFEQIHFDRFF"
     gene            673..2076
                     /locus_tag="PLH85_15130"
     CDS             673..2076
                     /locus_tag="PLH85_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB65950.1"
                     /translation="MNDARPPLPAPPRPVRRAAAALALAAVAGSAQATDVFRLEGFGA
                     VSRSMGGVATAQDVGPAGMMTNPATLSLLQAGHTLMGGLDLVTTDIEVQDQATGARVS
                     SGHHGSNRGPYVAPELAYAQRVGAWTFGVGAFAQGGLGTEYGSSSFLSRATGGLDTGL
                     DNSSRLLVLNIPLAASFKVNEALTVGAGIDAMWQGLNLNLLLGADQVGSLIGASRVSG
                     ALLPVLGGLPDLRGAHFSLTRNSPLASGINAWGHAERIGMTYQLRPDTRLGAAFTTRS
                     HMADMEGRATLTAVDGVAGQIPLNGNLKIRDFQMPAVLNLGVSHTVSERLQVAADLSR
                     VFWSRVMKDIKVGFVSDGGGDLNILLPQDYKDQNVLAVGAAYQWDARWTWRGGFRIAD
                     QALRGSTLFAVIPATPRKHLSAGFGYAVSPAASVDLAWSHAFREHLDNTSLPNTSAPI
                     GVNHAQDNATIAFTWRF"
     gene            2181..5072
                     /locus_tag="PLH85_15135"
     CDS             2181..5072
                     /locus_tag="PLH85_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65951.1"
                     /translation="MRPAECKDRVTARTPVHGVVIEPTDSAEVSRQKMARIILDSMYQ
                     FLGLLDVDGTVLEINRAALDGAGLDLADVIGQPFWHARWWAVSEEVRQRVRDMIAEAR
                     SGRFVRCDFEVFGDAHGSRTIVIDFSLTPILDDAGQVAYLLPEGRNITEKIARNAELS
                     RKNGELQVALERLKELDGYKTRFFANVSHELRTPLALILGPVDQLIQDGQALAERERF
                     RLAAIQRNARALLQQVNNLLDLARIDADRMPMAYVHANLAALLREVAAGFDAVAEDRG
                     IRVSLRGEDELYADVDRAKFMRVLSNLLSNAFKFTPSGGRIGCSVERLAGGRVLVSVQ
                     DTGPGVPAAMKAAIFDRFVQGGDGLGPGGSGLGLNIVKEFVELHRGTVVVLDAPGGGA
                     LFQVELPARAPEGAFVRSAGRDAAPADLAAPLAPELPPLTELPFHPGKRRVLVAEDNP
                     DLRQFLYDVLSDEYNVILRDDGEAAFRAALADPPDLIITDLMMPRWDGERFVRALAEQ
                     PDFPPVPVLVLSARADDALRERLLEHLVQDYLIKPFSARELRARARNLVTVKRTVDIL
                     QKELNSQASDILELTAGLVESRKSLQRSLTALQASDRRWLGLYDNTAVGIALADRDGR
                     IVSANPALQRMLGYGREDIVGVSLIDITEASERARTRQNVEGLFGGGNADYSVQKRYE
                     KRDGGYLWAHVVASRIPAAAEDGPLLAVIVEDITAHMEAERSLAATRAELARVARFTA
                     MGELVASIAHEINQPLSAVITNSQAALRWLSSPADAHLDEVAAALRRVNRDASLAGAV
                     IARIRNFLRAGDLRREPIDVARMLDELLQMLQLMLTDARVRVVVELPEPPPIVCADPV
                     QLQQVILNLTVNAVDAMREVQDRERLLTLSVHRDPAAGLRFDFRDSGAGVAEGTEARL
                     FDALYTTKPCGLGMGLAISRSIVENHGGRLWLDRKAGPGACFAFTIPDG"
     gene            5116..5532
                     /locus_tag="PLH85_15140"
     CDS             5116..5532
                     /locus_tag="PLH85_15140"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNB65952.1"
                     /translation="MTDTTLTGGCACGAVRYTSTAAPVLTAHCHCRDCQRASGAAYAT
                     VCAVPRAAFRVDQGETRSYRSIGDSGHGVVRHFCPTCGAPLFSDVEVAPDLSFLRVVS
                     LDDPAVVTPTAHVYCASAQPWDTHPDGLPAFPRMPG"
     gene            5529..6173
                     /locus_tag="PLH85_15145"
     CDS             5529..6173
                     /locus_tag="PLH85_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB65953.1"
                     /translation="MSGSAPMPLRDGAERVYVVDDDLSVREAVCSLIRSVGLRVETFP
                     SAAAFLAAPRDDGPACLILDVRMPGLSGLELQTELHRRGTSLPIIFVTGHGDIPMAVH
                     TIKKGAVEFLSKPFRDEDLLAAIDVALAQARSACSAGRELGEIRRRYAQLTAREKEVI
                     AFMVKGALNKQAAAELGVSEMTVKVHRRNVMHKMGVARLPDLVRMMERLQGAGG"
     gene            6287..>7024
                     /locus_tag="PLH85_15150"
     CDS             6287..>7024
                     /locus_tag="PLH85_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HNB65954.1"
                     /translation="MIRNRFKERLRAGELLYGFWLGLSEPFTAEICAGAGFDWLLIDG
                     EHGPNDLRTIFAQLQAIAPYASAPVVRPPQGDHVLIKQLLETGVQTLLIPMVESAAQA
                     RHLVEAMHYPPEGIRGVGSSIARASRWGRIADYSARANAEMCLLVQVETRAGMDALDE
                     ILAVPHVDGVFFGAADLAASYGLLGQPNHPDIVARIEAGLRAARARDMPGGVLCGDPA
                     LVQRYRDAGARFIAIGVDSMLLAAATSK"
     assembly_gap    7025..7124
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7148..8329)
                     /locus_tag="PLH85_15155"
     CDS             complement(7148..8329)
                     /locus_tag="PLH85_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB65955.1"
                     /translation="MTSPSPTASIPRSPAAAPVLSTRVADLHPSPIREILAVAGQPGM
                     ISFAGGLPAAETFPRFGVAAPDTVWQYGPTEGDPALRARIAVELGALGVDAAAERVLV
                     LSGSQQGIDLVGKLFVDAGTPVAVESPTYLAALQVFRFFGARFRPFAPDSLSAADWQS
                     DRPAFAYAIPTFQNPTGRCYSAAERIALAAACRDAGVPMFEDDPYRDLVYDACERRPV
                     CAELAGGTWIYQGSFSKSLAPGLRLGYLAASADLFPYLVRLKQAADLHSNRLSQWLVG
                     EQLDAPERPQRLARLADFYRAKRDAFEAALRRHFGALADWHTPAGGLFFWLTLKRPVD
                     TRLLLPAALARGVAFMPGEAFYPDAPGATGTLRLNFSHAAEADMDRGLAVLAELVAAA
                     E"
     gene            8451..8915
                     /locus_tag="PLH85_15160"
     CDS             8451..8915
                     /locus_tag="PLH85_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB65956.1"
                     /translation="MIDQNRPQALREAIELFFFAYRTFTAPPDRILAERGLGRVHHRI
                     LYFVGRNPDIAVSALLGVLGVSKQALNAPLRDLQAQGLVTATAAAHDRRVKQLALSPA
                     GAALEAELSGTQMAMLEAVFGAAGPQAEAAWRTVMASLVESARPEGARPPSP"
     gene            complement(8992..9909)
                     /locus_tag="PLH85_15165"
     CDS             complement(8992..9909)
                     /locus_tag="PLH85_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB65957.1"
                     /translation="MNTPAPLPGIPIAAVERDTGLSKDLLRVWERRYGFPTPLREAGG
                     ERSYPPEQLEKLRLIKRLIDHGLRPARLMGASTETLAGLLDGCAAPGGADRCAEFEAL
                     LRRVRLRHHVELRCTLQQMLLKHGLQRFVCEVVAPMNARIGDAWLRGEIEIADEHLYT
                     EHVQNLLRTAIGTQPAAGLQRPRVLLTTFPDERHALGLLMVEAMLTSEGAACLSLGTQ
                     TPVRDIAGAAAAGEADIVALSFSIAHPLRPALDGLRALREALPAKVDLWVGGAGVCGR
                     QDRLPGIRVIERIEDCVDALGVWRAAHPA"
     gene            9994..10203
                     /locus_tag="PLH85_15170"
     CDS             9994..10203
                     /locus_tag="PLH85_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65958.1"
                     /translation="MKSSVALLPPLAAAGVLLVGLCQCLGAAVAASAPCGAEACPVQA
                     APRVPDAAAELDVKLFLHTLSPGAR"
     gene            10203..10763
                     /locus_tag="PLH85_15175"
     CDS             10203..10763
                     /locus_tag="PLH85_15175"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNB65959.1"
                     /translation="MRPTVLRLLVSLGLALAAASAHAADACPGLLDHTLPRLQDDKPQ
                     SLCDYRGKVVLIVNTASYCGFTGQYDGLEKLYARLQPRGLVVLGFPSNQFGNQEPGSN
                     KEIAEFCRLTYGVKFPMFAKTEVIGSAAHPLFRQLASLTGERPKWNFHKYLIARDGRT
                     VRSFPSTTEPTDSTFVAAIEDALGRP"
BASE COUNT         1530 a         3982 c         3692 g         1533 t
ORIGIN      
        1 ttgccgccga cggtccggcc gacttcctgc ccggccagta cgccatgctg gatctgccgg
       61 gcgtcggcgc ctctcgcgcc tattccctgt ccaacacgcc caacgaccac ggcgaatggc
      121 acttccagat ccgccgcgtg gcgggcggac gcggcaccgc ggtgctcttc gacacgctgc
      181 gcccgggcca ggaggtgggc ctcgacggtc cctacggcgt ggcctacctg cggcccgacg
      241 cgccccgcga cctggtctgc gtggccggcg gttccggcct cgcgccgatg gtctccattg
      301 cccgcggcgc ggcccgcgcc ggcctgctca agacccgtcg cctgcacttc ttctacggcg
      361 cgcggacgcc ccgcgacgtg tgcgggcagg atttcctcgc cgccctgtcc ggcttcggcg
      421 agcgcgtcac gtacaccccg gtggtgtccg cccccgaggg gacggactgg gacggcgcca
      481 cgggcttcgt ccacagccac atggccgcgg ccctgcccgg tccgctgagc cgctacgagt
      541 tctacttcgc cggcccgccg cccatgaccc aggccctcca ggagctgctc atggtggatc
      601 accgggtgcc cttcgagcag atccatttcg accgcttttt ctgatcgcac aacactacac
      661 ggggagacat ccatgaacga cgcacgccca ccccttccgg cgccgccgcg ccctgtccgg
      721 cgcgccgccg cggccctggc cctcgctgcg gtggccggtt ccgcccaggc caccgacgtc
      781 ttccgtctgg aaggcttcgg cgccgtgtcc cgcagcatgg gcggcgtcgc caccgcccag
      841 gacgtgggac cagccggcat gatgaccaat ccggccaccc tgtccctgct ccaggccggg
      901 cacaccctga tgggcgggct tgatctggtg accaccgaca tcgaggtgca ggaccaggcc
      961 accggcgcgc gcgtgtcctc cggccatcac ggcagcaacc ggggccctta cgtggcgccg
     1021 gagctggcct acgcccagcg tgtcggcgcc tggaccttcg gcgtcggcgc cttcgcccag
     1081 ggcgggctgg gcaccgagta cggcagcagc agcttcctgt cccgcgccac cggcgggctc
     1141 gataccggcc tcgacaattc cagccgcctg ctggtgctga acatcccgct ggcggcgagc
     1201 ttcaaggtca atgaagcgct caccgtgggg gccggcatcg acgccatgtg gcagggcctc
     1261 aacctcaacc tgctcctggg cgcagaccag gtgggcagcc tgatcggcgc gtcgcgggtg
     1321 tccggcgccc tgctgcccgt gctgggcggt ctgccggacc tgcgcggcgc ccacttcagc
     1381 ctgacccgca attcacccct cgccagcggc atcaacgcct ggggccacgc cgagcggatc
     1441 ggcatgacct accagctgcg gcccgacacg cgcctggggg ctgccttcac cacccgcagc
     1501 cacatggccg acatggaagg ccgtgccacg ctcactgccg tggacggcgt ggcgggccag
     1561 attcccctca acggcaacct gaagatccgc gacttccaga tgccggcggt gctcaacctg
     1621 ggcgtcagcc acaccgtgtc ggagcggctc caggtggcgg cggacctgtc ccgggtgttc
     1681 tggtcccgcg tcatgaagga catcaaggtg ggcttcgtgt ccgacggcgg cggcgacctg
     1741 aacatcctgc tgccccagga ctacaaggac cagaacgtcc tcgccgtggg cgccgcctac
     1801 cagtgggacg cgcgctggac ctggcgcggc ggcttccgca tcgccgatca ggcgctgcgg
     1861 ggctcgactc tcttcgcggt gattccggcc acgccccgca agcacctgtc ggcgggcttc
     1921 ggctacgcgg tgtcgccggc ggcgagcgtg gacctggcct ggtcccacgc cttccgggag
     1981 catctggaca acaccagcct gcccaacacc tcggcgccca tcggcgtgaa ccatgcccag
     2041 gacaacgcca cgatcgcctt cacctggcga ttctgaaggc gcatgcttgc agggcgggtc
     2101 gggcgcctgc gcgagggtgc gcggtggccc ggtctgcgca cgtcccgagg cggcgcaggg
     2161 cgaggagacg agcacacgac atgagacccg ctgaatgcaa ggaccgggtg acggcgcgca
     2221 cgccggtgca cggcgtcgtc atcgaaccga ccgacagcgc ggaggtgtcg cgtcagaaga
     2281 tggcgcgcat catcctcgac tccatgtacc agtttctcgg cctgctggac gtggacggca
     2341 ccgtgctgga gatcaaccgg gccgccctcg acggcgccgg cctggacctg gccgacgtga
     2401 tcggccagcc cttctggcac gcccgctggt gggccgtctc cgaagaggtg cgccagcgcg
     2461 tgcgcgacat gatcgccgag gcccgcagcg ggcgcttcgt gcgctgcgac ttcgaggtct
     2521 tcggcgacgc ccatggcagt cgcaccatcg tcatcgactt ttcccttacg cccatcctgg
     2581 atgacgccgg gcaggtcgcc tacctgttgc cggaggggcg caacatcacc gagaagatcg
     2641 cccgcaacgc cgagctgtcg cgcaagaacg gcgagctgca ggtcgccctc gaacgcctca
     2701 aggagctgga cggctacaag acccgcttct tcgccaacgt cagccacgag ctgcgcacgc
     2761 ccctggccct gatcctcggc ccggtggatc agctcatcca ggatggccag gccctcgccg
     2821 agcgggagcg cttccgcctc gctgcgatcc agcgcaacgc ccgcgccctg ctgcaacagg
     2881 tgaacaacct cctcgacctg gcccgcatcg acgccgaccg catgcccatg gcctacgtgc
     2941 acgccaacct ggcggccctg ctgcgggagg tggccgccgg cttcgacgcg gtggcagagg
     3001 atcggggcat ccgtgtcagc ctgcggggcg aggacgagct ctacgcggac gtggaccggg
     3061 ccaagttcat gcgcgtcctg agcaatctgc tgtccaacgc cttcaagttc accccatccg
     3121 gcgggcgcat cggctgcagt gtcgagcgct tagccggcgg ccgcgtgctg gtgagcgtgc
     3181 aggacaccgg gccgggcgtg ccggcggcca tgaaggcggc catcttcgac cgcttcgtgc
     3241 agggcggcga cgggctcggg cccggcggca gcggcctggg tctcaacatc gtcaaggagt
     3301 tcgtcgagct gcacaggggc accgtggtgg tgctggacgc ccccggcggc ggcgccctgt
     3361 tccaggtgga actgcccgcc cgcgcgccgg aaggcgcctt tgtgcgcagc gccggccgcg
     3421 acgcggcgcc ggctgacctc gccgcgcccc tcgcgccgga actgccgccc ctgaccgagc
     3481 tgcccttcca tcccggcaag cggcgcgtgc tggtggcgga ggacaatccg gacctgcgcc
     3541 agttcctcta cgacgtgctc tccgacgagt acaacgtgat cctgcgggac gacggcgagg
     3601 cggccttccg cgcggccctc gccgatccgc cggatctcat catcaccgac ctgatgatgc
     3661 cccgctggga cggcgagcgc ttcgtgcggg ccctcgccga gcagccggat tttccgcccg
     3721 tgccggtgct cgtgctgtcc gcccgcgccg acgacgccct gcgcgagcgc ctgcttgaac
     3781 acctggtcca ggactacctg atcaagccct tctcggcgcg ggagctgcgc gcccgggcgc
     3841 gcaacctggt gaccgtcaag cgcaccgtgg acatcctgca gaaggagctc aactcccagg
     3901 cctccgacat cctcgagctg accgccggtc tggtggagag ccgcaagtcc ctgcagcgca
     3961 gcctgaccgc cctgcaggcc tcggaccggc gctggctggg cctctacgac aacaccgcgg
     4021 tgggcatcgc cctggccgac cgggacgggc gcatcgtctc cgccaacccg gccctgcagc
     4081 gcatgctcgg ctatggacgc gaggacatcg tcggcgtgtc cctgatcgac atcaccgaag
     4141 cctccgagcg ggcccgcacg cgccagaacg tggagggcct gttcggcggc ggcaacgccg
     4201 actacagcgt gcagaagcgc tacgaaaagc gcgacggcgg ctacctgtgg gcccacgtgg
     4261 tggcctcgcg catccccgcc gccgccgagg atggccccct gctggcggtg atcgtcgagg
     4321 acatcaccgc gcacatggag gccgagcgct ccctcgccgc cacccgcgcg gagctcgccc
     4381 gcgtggcccg cttcaccgcc atgggcgagc tggtggcctc catcgcccac gagatcaacc
     4441 agcccctgtc ggcggtcatc accaacagcc aggccgcgtt gcgctggctg tccagcccgg
     4501 ccgacgccca cctggacgag gtggccgccg ccctgcgccg cgtcaatcgg gacgccagcc
     4561 tggccggcgc ggtgatcgcc cgcatccgca acttcctgcg cgccggcgac ctgcgccgcg
     4621 aacccatcga cgtggcgcgc atgctcgacg agctgctgca gatgctccag ctcatgctca
     4681 ccgatgcccg cgtgcgcgtc gtcgtcgagc tgcccgagcc gccgccgatc gtctgcgccg
     4741 acccggtgca gctccagcag gtcatcctca acctcaccgt caatgccgtc gatgccatgc
     4801 gcgaggtgca ggaccgggaa cgcctgctca ccctctccgt ccaccgcgac ccggcggccg
     4861 ggctgcgctt cgacttccgc gacagtggcg ccggcgtggc cgagggcacc gaggcgcggc
     4921 tcttcgacgc gctgtacacc accaaaccct gcggcctggg catgggcctg gcgatcagcc
     4981 gcagcatcgt cgagaaccac ggcggccgcc tgtggctcga ccggaaggcg gggccgggcg
     5041 cgtgcttcgc cttcacgatc ccggacggct gacaccgtcc cgcgcccgcg ccgaccctga
     5101 accgaggaga tccccatgac cgacacgacc ctcaccggcg gctgtgcctg cggtgccgtc
     5161 cgctatacca gcacggccgc gccggtgctc accgcccact gccattgccg cgactgtcag
     5221 cgcgccagcg gcgccgccta cgccaccgtg tgtgcggtcc cccgcgcggc ctttcgcgtg
     5281 gatcagggcg agacgcgcag ctaccgctcc atcggcgaca gcggccacgg ggtggtgcgg
     5341 cacttctgcc cgacctgcgg tgcgccgctc ttctccgacg tggaagtggc gccggacctg
     5401 agcttcctgc gcgtcgtgag cctggacgat cccgccgtcg tcacccccac ggcccacgtc
     5461 tactgcgcca gcgcccagcc ctgggatacc cacccggacg gcctgcccgc ctttccgcgg
     5521 atgcccggat gagcggcagc gcgccgatgc cgctccgcga cggggccgag cgggtgtacg
     5581 tggtggacga cgacctctcc gtgcgcgagg ccgtgtgcag cctgatccgc tcggtgggcc
     5641 tgcgcgtcga gaccttcccg tcggccgccg cctttctcgc tgcgccgcgg gatgacggac
     5701 cggcctgcct gatcctcgac gtgcgcatgc cgggtctcag cggtctggag ctccagaccg
     5761 agctgcaccg ccggggcacc tcgctgccca tcatcttcgt caccggccac ggggacatcc
     5821 cgatggcggt gcacaccatc aagaaggggg cggtggagtt cctgtccaag cccttccgcg
     5881 acgaggacct gctcgccgcc atcgacgtgg ccctggccca ggcccgcagc gcctgctcgg
     5941 ccggacgcga gctgggcgag atccgccgcc gctacgccca gctcaccgcc cgcgagaagg
     6001 aggtcatcgc cttcatggtg aagggcgccc tcaacaagca ggcggcggcc gaactgggcg
     6061 tgtcggagat gaccgtcaag gtgcaccgcc gcaacgtcat gcacaagatg ggcgtggcgc
     6121 ggctgcccga tctggtgcgc atgatggagc gtctgcaggg cgccggtggc tgaggggggc
     6181 cggcccgcgc gacggcgtcg gcgtttgccg ctacagtcgt ggcgcgacgc cgggtggcgg
     6241 cccgtccggc aacaagagga acgatcagga ggatgacagg gtggagatga tccgcaaccg
     6301 cttcaaggaa cgcctgcgcg cgggcgagct gctctacggc ttctggctcg gtctgtccga
     6361 gcccttcacc gccgagatct gcgccggcgc cggcttcgac tggctgctca tcgacggcga
     6421 gcacggcccc aacgacctgc gcaccatctt cgcccagctc caggccatcg cgccctatgc
     6481 ctccgcgccg gtggtgcgtc cgccccaggg cgaccacgtg ctcatcaagc agctgctgga
     6541 gaccggcgtg cagaccctgc tgatccccat ggtggagtcg gcggcccagg cgcgtcacct
     6601 ggtggaggcc atgcactacc cgccggaggg catccgcggt gtgggcagct ccatcgcgcg
     6661 ggcctcccgc tgggggcgca tcgccgacta cagcgcccgc gccaacgcgg agatgtgcct
     6721 gctggtgcag gtggagaccc gcgccggcat ggacgccctg gacgagatcc tggcggtgcc
     6781 ccacgtggac ggcgttttct ttggcgccgc cgatctggcc gcctcctacg gcctgcttgg
     6841 ccagcccaac cacccggaca tcgtggcgcg catcgaggcc ggcctgcgcg ccgcccgggc
     6901 gcgggacatg cccggcggcg tgctgtgcgg cgacccggcc ctggtgcagc gctaccggga
     6961 cgcgggcgcc cgcttcatcg ccatcggcgt ggattccatg ctcctcgccg cggccacctc
     7021 gaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaggcg cgcggaccgc
     7141 cgcagcgtca ctcggccgcg gcgaccagtt ctgccagcac cgccagtccc cggtccatgt
     7201 ccgcctcggc ggcgtggctg aagttgaggc gcagggtgcc ggtggcgccc ggcgcgtccg
     7261 ggtagaaggc ctcgccgggc atgaaggcga cgccgcgggc gagggcggcc ggcagcagca
     7321 ggcgggtgtc caccgggcgc ttgagggtga gccagaagaa cagcccgccg gccggcgtgt
     7381 gccagtccgc cagggcgccg aagtggcggc gcagggcggc ttcgaaggcg tcccgcttgg
     7441 cccggtagaa gtccgccagg cgggccaggc gttgggggcg ctccggcgcg tcgagctgtt
     7501 cgcccaccag ccactggctc aggcggttgc tgtgcaggtc ggccgcctgc ttgaggcgca
     7561 ccaggtaggg aaacaggtcg gccgaggccg ccagatagcc caggcgcagg cccggcgcga
     7621 gactcttgga gaaggagccc tggtagatcc acgtcccgcc ggccagctcg gcgcacaccg
     7681 ggcggcgctc gcaggcgtcg tagaccagat cccggtaggg gtcgtcctcg aacatgggca
     7741 cgcccgcgtc gcggcaggcg gcggccaggg cgatccgctc ggcggcgctg taacagcgcc
     7801 cggtggggtt ctggaaggtg gggatcgcgt aggcgaaggc cggccggtcc gactgccagt
     7861 cggctgcgct caggctgtcc ggcgcgaagg gccgaaagcg cgcgccgaag aagcggaaca
     7921 cctgcagggc ggccaggtag gtgggcgact ccaccgccac cggggtgccc gcatccacga
     7981 acagcttgcc caccaggtcg atgccctgct gggagccgga cagcaccagc acccgctccg
     8041 ccgccgcatc cacgccgagg gccccgagct ccacggcgat gcgcgcccgc agggccgggt
     8101 cgccttccgt cgggccgtac tgccacacgg tgtccggcgc ggccacgccg aagcgcggaa
     8161 aggtctctgc cgcgggcagg ccgccggcaa aggagatcat gcccggctgc ccggcgacgg
     8221 ccaggatctc ccggatcggc gacgggtgca ggtctgccac gcgcgtggac aggaccggcg
     8281 cggcggcggg ggatcggggg atcgaggcgg tcggggaagg ggacgtcatc gtgggctccg
     8341 gttcggctgg gggatcgggt ggcggcgcag gggtggatgc cattgccgcc ggacagataa
     8401 gtcaataaac ttgaccataa atcctaggcg tccgaccgtt ataagtcaac atgattgacc
     8461 aaaatcgtcc ccaggccctg cgcgaggcca tcgagctctt cttcttcgcc taccggacct
     8521 tcaccgcgcc gccggaccgc atcctcgccg agcgcggcct cgggcgcgtc catcaccgca
     8581 tcctgtactt tgtggggcgc aacccggaca tcgcggtgag cgccctgctg ggggtgctgg
     8641 gggtcagcaa gcaggccctc aacgcaccgc tgcgggacct gcaggcccag ggcctcgtca
     8701 ccgccacggc ggcggcccac gaccggcgcg tcaagcagct ggccctgagc ccggccggcg
     8761 cggcgctgga ggcggagctc agcggcaccc agatggccat gctggaggcc gtgttcggcg
     8821 cggccggtcc gcaggccgag gcggcctggc ggacggtcat ggcgagcctg gtggaatcgg
     8881 cccggccgga gggggcccgc ccgccatccc cctgaggatg gccaacgggg cgccctgcgt
     8941 gcgtgtcggg cgcctcgggt ggcgcgggga cgacgcggcg atgtgggccg ttcaggccgg
     9001 gtgggcggcg cgccagacgc ccagagcgtc cacgcaatcc tcgatgcgct cgatcacccg
     9061 gatgcccggc agcctgtcct gacggccgca tacgccggcg ccgccgaccc acaggtccac
     9121 cttcgccggc agcgcctcgc gcagggcgcg caggccatcc agggccggcc gaaggggatg
     9181 ggcgatactg aaggacaggg cgacgatgtc tgcctcgccg gccgcggccg caccggcgat
     9241 gtcccgcacc ggcgtctgcg tgcccaggga caggcaggcc gcgccttccg aggtgagcat
     9301 cgcttcaacc atcagcaggc cgagggcatg acgttcgtcg ggaaaggtgg tcagcaggac
     9361 gcgcggccgc tgcagcccgg ccgccggctg ggtgccgatg gcggtgcgca gcaggttctg
     9421 gacatgctcg gtgtacagat gctcgtcggc gatctcgatc tcgccgcgca gccaggcgtc
     9481 gccgatgcgg gcgttcatcg gcgccaccac ctcgcacacg aagcgctgca ggccgtgctt
     9541 cagcagcatc tgctgcagcg tgcagcgcag ttccacgtgg tgacgcagac gcacgcggcg
     9601 cagcagggcc tcgaattcgg cacagcggtc ggctccgccg ggggcggcac agccgtcgag
     9661 caagccggcg agcgtctcgg tggaggcgcc catcagccgc gccgggcgca ggccgtggtc
     9721 gatcaggcgc ttgatcaggc gcagcttctc gagctgctcc ggcggataac tgcgctcgcc
     9781 gcccgcctcc cgcaaggggg tcggaaaacc gtagcgccgc tcccagaccc gcaggagatc
     9841 cttcgagaga cccgtgtccc gctcgacggc ggcaatcgga atgcccggca ggggtgcagg
     9901 cgtgttcatg tttgtcctgg acaatattgt tgacattggg ctgacaggct tctagcttag
     9961 gaatgtgtcc cattcatgtc caggacaaac atcatgaaat cgagtgtcgc ccttctccca
    10021 cccctcgccg cggccggcgt gctgctggtg ggcctgtgtc agtgcctcgg cgcggccgtc
    10081 gcggcctcgg caccctgtgg cgcagaggcc tgccccgtcc aggccgcgcc ccgtgtcccg
    10141 gacgccgccg ccgaactgga cgtgaagctc ttcctccaca ccctgtcgcc gggagcccga
    10201 tgatgcgacc gaccgtcctt cgccttctcg tgagcctggg gctggccttg gccgccgcct
    10261 cggcccacgc agccgacgcc tgccccggcc tgctcgacca caccctgccg cgccttcagg
    10321 acgacaagcc ccagtccctc tgcgactacc gcggcaaagt cgtgctcatc gtgaacaccg
    10381 ccagctactg tggcttcacc ggccagtacg acggcctcga aaagctctac gcgcgcctgc
    10441 agccccgcgg cctggtggtg ctcggcttcc cgtccaacca gttcggcaac caggagcccg
    10501 gcagcaacaa ggagatcgcc gagttctgcc gcctcaccta cggcgtcaag ttccccatgt
    10561 tcgccaagac cgaagtcatc ggcagcgccg cccatcccct gttccgccaa ctcgcgtccc
    10621 tcaccggcga gcgccccaag tggaacttcc acaaatacct gatcgcccgg gatggccgca
    10681 cggtacgcag ctttccgtcc accacggaac cgaccgatag cacctttgtg gcggccatcg
    10741 aggacgcgct ggggcggccc tgatccgttg ggggcggtgt ggggcgtggg ttgggtctgc
    10801 caacggtcag tcaaactccc gccgtgcgcc ccagcag
//
LOCUS       DAOQMM010000121        10367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3027_length_10367_cov_39.189294, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000121 DAOQMM010000000
VERSION     DAOQMM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3027_length_10367_cov_39.189294"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2170
                     /locus_tag="PLH85_15180"
     CDS             <1..2170
                     /locus_tag="PLH85_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNB65960.1"
                     /translation="AAAGQHASSFEAQDSLLATLLLGAAALLVAAGSFHLWRRAERAG
                     EIALSRQLAAQSATALAEAAPDTALRLAAAAGQHASSFEAQDSLLAALAQAPLDWRSA
                     GQQGLFSLAVRADGGLIASGGRDGSIRLWRGGDDLTPAARLDAHKEQVLALAFSPDGQ
                     RLASAGIDRRVILWDVRDPARPQRLAERADHEGPVLAVAFSPDGQRLASAGSDRRIVL
                     RDGHSGQPVGPVLDAHGDTVTGLAFSPDGRLLASTGYDGALLLWATDGTATPRLSASH
                     PAVLYGVAFSPDGNLIATAGADRHVVLWQSTTLAVRDAPLTDHDAAVRAVAFSPDGQR
                     LISVGDDRQVLEHDLTRPTSLGRNHPGHRDRALAVAFTAAGDAFSVGADDRLIHHGQT
                     LGSTLATRLPGVDLPAWALAWSPDGRQLASAGPDGAILLRDALSGAPVGQARRGHEAL
                     VTGLAFTPDGTLLASAGQDGRLFLWPVAGGPPRALQPAGEALQTVTVSPDGRTLAAAG
                     RDGHILRWTLPDATPLPRLEHGAAVSDLAFAPDGRRLASAGRDGTLRLWNADGTAADR
                     LDLPGDSLTRIAWPAGDGPLALATRTRGLLQWTPGDAAPRPLGNDEHALLALSFSPDG
                     RQLASADVNGRVRLWDMVSGQAFHAPVQRFPGPVLALAFAPDGRRLAGTGWGGGVQLT
                     PIGADLWSRMACERLRPLPPASPESACPAPENGRATTSRP"
     gene            2281..3711
                     /locus_tag="PLH85_15185"
     CDS             2281..3711
                     /locus_tag="PLH85_15185"
                     /inference="COORDINATES: protein motif:HMM:NF016845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNB65961.1"
                     /translation="MALIIPGVEVKVVKEVLSPQLAPSGVLGLVGIVEAPGSGIGRAA
                     SWRSFTDRFGAASAFSLPEAAQALGNGVFELVVCPVACDATASLKVPAATGNAAAFTL
                     SARAPGPWGNALQVSLATRQVATGKKLDSGDDETRQVFDLTIRIAGSDYKEVHRNLST
                     DATDTLRHVRDVLAAQSQLLRVVEERDKLKPPKDSPDVKDPSAALLGLTGGADADPDA
                     YRKAIALLENESDVDMVLVSVQDTRDTTRVGRIYADVISHCDRMSARSQGRIGFGEVP
                     GGNAADIATAADKLTGTLVSDRFILVAPAGVVGAVAGMTGSLDYFQSPTFKRLSGLGE
                     PAPALGVESQQELLRKNVVPVVAQRGRGIIVVRGLTTDGDQISVRRVADHAVRGVKTI
                     GELFIGRLNNEEGRGALKQKLVEFLVQMQKEGAIVPSTDGTDPAFKVNVYSSQQDFAQ
                     GIVRVDLAVRPVRAIDYIYATVLVQV"
     gene            3773..4165
                     /locus_tag="PLH85_15190"
     CDS             3773..4165
                     /locus_tag="PLH85_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65962.1"
                     /translation="MPTVFSANRSSVLVDGEAIEGLQSLAYRVVTEREDIRAVGSDER
                     VAVSFGLRTVQGELTVRSASYKLDGHLDKQAKFQLVANLKKDDAADSPKRTVSFDDCY
                     VEDKSFSLGAGGTVVTAYTFSATRVREE"
     gene            4170..4988
                     /locus_tag="PLH85_15195"
     CDS             4170..4988
                     /locus_tag="PLH85_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65963.1"
                     /translation="MGLREHLVAGTPCMLDFAGFDRPLGVLVGGALQRLAPWTYRDHL
                     DALRACTRLVGPALRLDAAAFADRVLAPLDLPAEERPSLHPIALWWAAGGDDPLLPPP
                     AEGDWLDLGSARARLRPWTEHQRLATLDGCLDADQDTPDATDSFDPVGYLDGMVRASL
                     AVLEGPVPLERLDARASAVLLNATVALNVVDEADDTLLQGGPAARLAATRTLRLCRAL
                     GWTPTRVWSTPAAEVDRLLRLLDLLEPPARPVPAHRGLADHPDATVIRFEDDAA"
     gene            5768..6175
                     /locus_tag="PLH85_15200"
     CDS             5768..6175
                     /locus_tag="PLH85_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65964.1"
                     /translation="MPRHDGVATPSPSAAAPSAAAAPPVDEVAPPAPAFLPRHPVLPR
                     IVAALDPVLEQAWRVTDAALPTAAALDAAPPPGTDTAASADPAGAPRVNNHFHVNVAL
                     GSDSAGSGRDPQQLEDALVTLLRDAARRQGLDV"
     gene            6172..7203
                     /locus_tag="PLH85_15205"
     CDS             6172..7203
                     /locus_tag="PLH85_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65965.1"
                     /translation="MNAPARRSAYTVRLGDAVASSTASESRRQLLRLQVVLDMDGPVA
                     ACTVELADAASPPPAIGDAVSVELDGGDGLQTVFTGTVAEAAAHATGQRIVAHGHFAP
                     LGAREVEAAYDSVAADFIVKDLLDQAGATAGTVAAGPDLAAWTLQRSPTALGHLRRLA
                     LLCGADLYADGRGAIHCAVPRSGAADHRFTFGENILRLQLARRVPAFDSIEVWGEGAD
                     QGADKAHWLSTDLAQVSAKASVAADGSVQTGKLGQRPLRLREGALRSGAAAEAVAKAW
                     ATRVAAGRVEGSVEVFAAPKVCPGDLVGIDKLPDDHPAAALLDGPLRVRSVRHELDRQ
                     RGAITRLGF"
     gene            7206..8087
                     /locus_tag="PLH85_15210"
     CDS             7206..8087
                     /locus_tag="PLH85_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HNB65966.1"
                     /translation="MTVRIGKIELTGVQDLHTEESRTLVEQRVPEQQGSVFQDLGREP
                     LTLVIDGLLLGEDALSALETLRQAHAKAEPQSFAADLVVGTDLTDVIIEDFRVRQVAG
                     YRDRYRFSLRLREHIEPPAAADAAVADVNQGVAADAAGWSDSSLAAAGVLQDPASLSA
                     ALAAQPGLLDTLDMDALGDAVAGNLDSLDAGQLDGMLGALANADPTKAGGLFDALGKA
                     GALGPMLAKYAQEGAAFLKKLDPSKLSSLMRAFSGGLEFLNQLKKVAEAAGKLAGDIK
                     DLRLPPELDRILKGGRP"
     gene            8084..8629
                     /locus_tag="PLH85_15215"
     CDS             8084..8629
                     /locus_tag="PLH85_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65967.1"
                     /translation="MSSTADVVRDLASLVRELGALLETKTAREIPAIARDLGAEAQLG
                     QGVDALASVLGQVRHGVVPLRRMAIGADALVAALGCVPPLVGGLARATDASGEWLATL
                     GLGLGDAAGAAQAVSGPIRQVSDVLDMGEDVAEGAVSLLAAGQWRGLITALDRLDTSL
                     KALKAPPPTPPGAPAGALSGG"
     gene            8635..9687
                     /locus_tag="PLH85_15220"
     CDS             8635..9687
                     /locus_tag="PLH85_15220"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNB65968.1"
                     /translation="MADTLPAPTSDGRADNPFRAVAILLDAVGRSLEASKRQDQAARW
                     VRIARVREFRDDGIAKGVMKAMDVLTLAVAYLQRFTLDANDLLVQGDAAKALVEVSAE
                     FIKTATSKEFINSLEMAVGQDPSPDSPIPDVANIIDKIVQIADKVPEPDDLKVIGAAL
                     WRLLGIEQASLDEAKLGATTESHIVVERTGKLRLLQFAFDQNFRLQNFGKGAGVSSLD
                     VNRLGGRRLWEAGADKLPSKSVGKYGDAPDIETVWELDFSTDKAGADVQDANSILEAL
                     GYADPAVPDRKVFGAEFAGRLRTFQQINEIPLSGKLDNATLNRLLHLDFDAKNLKRAK
                     RFKADALPKGFDPLKK"
     gene            9728..10366
                     /locus_tag="PLH85_15225"
     CDS             9728..10366
                     /locus_tag="PLH85_15225"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNB65969.1"
                     /translation="MSDPIDIIRAVVRDELRSLRLGDLAVVTSIFPHTGDDDGHNHEC
                     SVKLREGGLELRKVPIATPHVGMVGAPRVGDLVLVSYVGGDPNRAVVIGRLYSEKIHP
                     PLHKEDEWHAFAKADGVARYTIDGEESCIVTAGKTIVTVKKDGNVEIQGEADLAIQVK
                     GKVTIQCTDFSLDASGNVDLGQSGAGVITEQSHKCYFTGKALVPSGTVKAKG"
BASE COUNT         1506 a         4250 c         3262 g         1349 t
ORIGIN      
        1 cgccgcggcc ggtcagcacg cctccagctt cgaagcccag gacagcctcc tcgccaccct
       61 gctgctgggt gccgccgcgc tcctcgtcgc cgccggcagc ttccatctgt ggcggcgcgc
      121 cgagcgggcc ggcgagatcg ccctgtcgcg ccagctcgcc gcccagtccg ccactgccct
      181 ggccgaagcc gcccccgaca cggccctgcg tctggccgcc gcggccggtc agcacgcctc
      241 cagcttcgaa gcccaggaca gcctcctcgc cgccctcgcc caggcgcccc tcgactggcg
      301 cagcgccgga cagcagggac tcttcagtct cgccgtgcgc gccgatggcg gcctcatcgc
      361 cagcggcggg cgggacggca gcatccgcct gtggaggggc ggcgacgatc tgacgcccgc
      421 cgcccgcctc gacgcccaca aggagcaggt cctcgccctg gccttctcgc cggacggcca
      481 acgccttgcc agcgctggca tcgaccgccg ggtgatcctg tgggatgtgc gcgacccggc
      541 ccggccccaa cgcctcgccg aacgtgccga ccacgaaggc ccggtccttg ccgtggcctt
      601 ttcgccggac ggccagcgcc tcgccagcgc cggctccgac cgccgcatcg tcctgcgcga
      661 cggccacagc ggtcagcccg tcggtcccgt cctcgacgcc cacggcgaca ccgtcaccgg
      721 cctcgccttc agcccggacg gccgcctgct cgcctccacc ggttacgacg gcgccctgct
      781 cctgtgggcc accgacggca ccgccacgcc acgcctgagc gccagccacc ccgccgtgct
      841 gtatggcgtc gccttctcac ccgatggcaa tctgatcgcc acggccggtg ccgaccgcca
      901 cgtcgtcctg tggcagagca ccaccctcgc cgtgcgcgac gcaccgctca ccgaccacga
      961 cgccgcggtg cgggccgtcg ccttcagccc ggacggacag cgtctgatct ccgtcggcga
     1021 cgaccgccag gtgctggagc acgacctcac gcgccccaca agcctcggcc gcaaccatcc
     1081 cggccaccgg gaccgcgccc tggcggtcgc cttcacggcc gccggcgatg ccttcagtgt
     1141 cggtgccgac gaccgcctca tccaccacgg ccagaccctc ggttcgaccc tcgccacacg
     1201 cctgccgggc gtcgacctgc ccgcctgggc cctcgcctgg agcccggacg ggcggcagct
     1261 cgccagcgcc ggcccggacg gcgccatcct gctgcgggac gccctcagcg gtgcgcccgt
     1321 cggccaggcg cgacgcggcc acgaagccct cgtcaccggc ctcgccttca ccccggacgg
     1381 cacgctgctc gccagcgccg gccaggacgg ccgcctcttc ctgtggcctg tcgctggcgg
     1441 accgccacgc gccctgcagc cggccggcga ggcgctgcag accgtcaccg tcagcccgga
     1501 cggccggacc ctcgccgccg ccggccgcga cggacacatc ctgcgctgga cgctgcccga
     1561 tgccaccccg ctgccccgcc tggagcatgg cgctgcggtc agcgacctcg ccttcgcccc
     1621 ggatggccgc cggctcgcca gcgcggggcg cgacggcacc ctgcgcctgt ggaatgccga
     1681 cggcaccgca gccgaccgcc tggatctgcc cggcgacagc ctgacgcgca tcgcctggcc
     1741 cgccggagac ggccccctgg ccctggccac gcgcacccgc ggattgctgc aatggacgcc
     1801 cggcgacgcg gccccgcgcc ccctcggcaa cgacgaacac gccctgctcg ccctcagctt
     1861 ttcgccggac ggccgccagc ttgccagcgc agacgtgaac gggcgggtgc gcctgtggga
     1921 catggtcagc gggcaggcct tccacgcccc cgtccagcgc ttccctggcc cggtgctggc
     1981 cctcgccttc gcgcccgacg gtcgccgcct ggcgggcacc ggctggggcg gcggggtgca
     2041 gctcacgccc atcggggccg acctctggag ccgcatggcc tgcgaacgcc tgcgccctct
     2101 cccgccggcc tctccggaat ccgcctgccc cgcccccgag aacggccggg cgacgacatc
     2161 tcgaccttga catcggcaag tcccgatcta ctctggttag acggccggcc cagcagcgcc
     2221 gggccgtggc attctcatcc cgcgccaaat atatgccggc gccctttcgg agagcttccc
     2281 atggcattga tcatcccagg cgtcgaagtc aaggtcgtca aggaagtcct ctcgccccaa
     2341 ctggccccct ccggcgtgct ggggctggtc ggcatcgtcg aggcgccggg cagcggaatc
     2401 ggtcgcgccg ccagctggcg cagcttcacc gaccgcttcg gcgccgccag tgccttcagc
     2461 ctgcccgaag ccgcccaggc gcttggcaac ggtgtcttcg aactggtcgt atgcccggtc
     2521 gcctgcgacg ccaccgccag cctcaaggtg cctgccgcca ccggcaacgc cgccgccttc
     2581 accctcagcg cccgcgcgcc gggcccgtgg ggcaatgcgc tccaggtcag cctcgccacc
     2641 cgccaggtgg ccaccggcaa gaagctcgac agtggcgacg acgagacccg gcaggtcttc
     2701 gatctgacca tccgcatcgc cggcagcgac tacaaggaag tacaccgcaa cctgtccacc
     2761 gatgccaccg acaccctccg ccacgtgcgc gacgtactcg ccgcccagtc ccagctcctg
     2821 cgcgtcgtcg aagaacgcga caaactcaag ccccccaagg acagcccgga cgtcaaggat
     2881 cccagtgccg cgctgctggg tctgaccggc ggcgccgacg ccgaccccga cgcctaccgc
     2941 aaggccatcg cgctgctgga aaacgaatcc gacgtggaca tggtgctggt ctcggtgcag
     3001 gacacccgcg acaccacccg ggtcggccgc atttacgccg atgtgatcag ccactgcgac
     3061 cgcatgagcg cccgcagcca gggccgcatc ggcttcggtg aagtgcccgg cggcaacgcg
     3121 gcggacatcg ccaccgcggc cgacaagctc accggcaccc tcgtctccga ccgcttcatc
     3181 ctcgtcgccc cggccggcgt ggtgggcgcg gtggccggaa tgaccggcag cctcgactat
     3241 tttcagtcgc ccacgttcaa gcgtctcagc gggctgggcg agccggcccc ggccctcggt
     3301 gtcgaatccc agcaggagct cctgcgcaag aacgtggtcc ccgtcgtcgc ccagcgcggc
     3361 cgcggcatca tcgtcgtgcg cggcctcacc accgacggcg accagatcag cgtgcgccgc
     3421 gtggccgacc acgccgtgcg cggcgtcaag accatcggcg aactcttcat cggacgcctc
     3481 aacaacgagg aaggccgcgg cgccctcaag cagaagctcg tcgaattcct cgtccagatg
     3541 cagaaggaag gcgccatcgt gccctccacg gacggcaccg accccgcctt caaggtcaat
     3601 gtctattcca gccagcagga tttcgcccag ggcatcgtgc gtgtggacct ggccgtgcgc
     3661 ccggtgcgcg ccatcgacta catctacgcc accgtgctgg tccaggtctg acgcccggca
     3721 gcaccccgcg acataagatc ccgcaccctc gacatctccg gagagtcccg ccatgccgac
     3781 cgtcttttcc gccaaccgct ccagcgtcct ggtcgatggg gaagccatcg aaggcctgca
     3841 atccctggcc taccgggtcg tcaccgaacg ggaggacatc cgtgccgtgg gcagcgacga
     3901 gcgcgtcgcc gtgagcttcg gcctgcgcac cgtgcagggc gagctcaccg tacggtccgc
     3961 cagctacaag ctcgacggcc atctggacaa acaggccaag ttccagctcg tcgccaacct
     4021 caagaaggac gatgccgccg attcacccaa gcgcaccgtc tccttcgacg actgctacgt
     4081 ggaggacaag tccttcagcc tgggcgccgg tggcaccgtg gtgaccgcct acaccttcag
     4141 cgcgacccgc gtccgcgagg agtagaccct tgggcctgcg cgagcatctc gtcgccggca
     4201 ccccctgcat gctggatttc gcgggcttcg accggcccct tggcgtgctc gtgggcggcg
     4261 cactccagcg cctggcgccg tggacctacc gggatcacct ggacgccctg cgcgcctgca
     4321 cccggctggt cggcccagcc ctgcgcctgg atgccgccgc ctttgccgac cgggtcctcg
     4381 cgcccctcga cctgcccgcc gaagagcgtc cgtcgctgca tcccatcgcc ctgtggtggg
     4441 cggcgggcgg agacgacccc ctgttgccgc cgcccgccga gggcgactgg ctcgacctgg
     4501 gcagcgcccg cgcccggctg cgcccgtgga ccgaacacca gcgcctcgcc accctcgatg
     4561 gctgcctcga tgccgaccaa gacaccccgg acgcaaccga cagcttcgac cccgtcggct
     4621 acctggacgg catggtccgc gccagcctcg ctgtcctcga gggccccgtc ccactggaac
     4681 gcctcgacgc ccgcgccagc gccgtcctgc tcaacgccac ggtcgccctc aatgtcgtgg
     4741 acgaggccga tgacaccctg ctgcagggtg gcccggccgc ccgtctggcc gccacccgca
     4801 ccctgcgcct gtgccgcgcc ctcggctgga cgcccacgcg cgtctggagc acccccgcgg
     4861 ctgaagtgga ccgcctgctg cgcctgctcg acctgctgga gccaccggcc cgcccggtgc
     4921 ccgcccaccg tggcctggcc gatcacccgg acgccaccgt gatccgtttc gaggacgacg
     4981 ccgcatgagc accgcccaca cggagcgcct cgtcgcccgc ctgctcgcgc cccttgcccg
     5041 cagtctcgac ggcgcgcgcc gccacctcga cctggcggac ccggatgccg acgcgactga
     5101 cgacgcccgc ccggagccca cggacggact gccagcacgg cccgcttttc ccgcccgtgt
     5161 cgccccgctg acgcccgcca tcgagacggt cgcgccctcg cgtcccaccg cccctgccgt
     5221 cattgcccgg cccccggaac aaccgacgac cgacgcctgg ccgcccgcat ccgctccgcg
     5281 ggccccggcc acggcgccca ccccgccgga tcgggcggca gcggccccgc acagtcggcc
     5341 caccccggcg agcacagccc agcgcgtccc cacccacgcg cccgaggcgg gcctgggcga
     5401 tcggcgcgcc ccccctcctc cgataccggt ggccgaacga atgtcgcaca cagcaccgga
     5461 tgtcacccac cgcccccccg aggggatgca cacacaggtg cccggcgatc cgctgcccac
     5521 tgaacccgct ccccaggtct ccctccgcgt ccccctcgtc agggcacgcg ttcaggacgc
     5581 gcacggcgtt gatgcacgca gcccggggat gtcagccccg atcatcggag cgcaagcgcc
     5641 tgacgtgaca cgccccggcg atgccgcccc cgccgcgccc acggcccctt ctgcgcctcc
     5701 tgcgtcgcgg ggcgcggtcc cagccgcaac cggcacacct gccctccgtc tccccgtacg
     5761 ccccgccgtg ccccgccacg atggggtcgc cacaccttcg ccctctgccg ccgcgccgtc
     5821 cgccgcggcg gcacccccgg tcgacgaggt cgcgcccccg gcaccggcct tcctgccccg
     5881 ccatcccgtc ctgccccgca tcgtggccgc cctcgatccc gttctggagc aggcctggcg
     5941 cgtcaccgac gcagccctgc ccacggccgc cgcgctcgac gccgccccgc cccccggcac
     6001 ggacaccgca gcgtctgcag acccagccgg cgccccgcgg gtcaacaacc atttccacgt
     6061 caacgtcgcc ctcgggagcg acagcgcagg cagcggccgc gatccccagc agctcgaaga
     6121 cgccctcgtc accctgctgc gcgacgcggc ccgccgtcag gggctggacg tatgaacgcc
     6181 ccggcccggc gcagcgccta cacggtccgc cttggcgacg cggtggccag cagcacggcc
     6241 agcgagtccc gccgccagct cctgcgtctg caggtggtgc tcgacatgga cggcccggtc
     6301 gccgcctgca ctgtcgaact ggccgacgcc gccagcccgc cccccgccat cggcgacgcg
     6361 gtaagcgtcg agctcgacgg cggcgacggc ctgcagaccg tcttcacagg caccgtcgcc
     6421 gaagccgctg cccacgccac cggccagcgc atcgtcgccc acggccactt cgcgcccctc
     6481 ggtgcccgcg aggtggaagc cgcctacgac agcgtcgccg cagatttcat cgtcaaggac
     6541 ctgctggacc aggctggcgc cacggccggc acggtcgctg ccgggccgga tctggcggcg
     6601 tggacgcttc agcgcagccc caccgcgctt ggccatctgc gccgcctggc cctgctctgc
     6661 ggcgccgacc tctacgccga cggccgcggc gccatccact gcgctgtccc ccgtagcggc
     6721 gcggcggatc accgtttcac gttcggcgag aacatcctgc gcctgcagct ggcgcgccgc
     6781 gtgccggcct tcgacagcat cgaggtgtgg ggggaaggcg ccgaccaggg cgcggacaag
     6841 gcccactggc tgagcaccga cctggcgcag gtgtctgcca aggcgtccgt tgccgccgac
     6901 ggcagcgtgc agaccggaaa gctcgggcaa cgccccctgc gcctgcggga aggcgccctg
     6961 cgcagcggcg ctgccgccga agccgtcgcc aaggcctggg ccacgcgggt cgccgcgggc
     7021 cgggtggaag gcagcgtcga agtcttcgcc gcgcccaagg tgtgccctgg cgacctcgtc
     7081 ggcatcgaca aactgccgga cgaccacccc gccgccgccc tcctcgacgg tcccctgcgg
     7141 gtgcgcagcg tgcgtcacga gctcgaccgc cagcgcggcg ccatcacccg gctggggttc
     7201 tgaccatgac cgtgcgcatc ggcaagatcg aactcacggg cgtgcaggac ctgcacaccg
     7261 aggaatcgcg caccctcgtc gaacagcgcg tgcccgagca gcagggcagc gttttccagg
     7321 accttggccg cgagcccctc accctggtca tcgacggtct gctgctgggc gaggacgccc
     7381 tgtcggcact cgagaccctg cgccaggccc acgccaaggc cgaaccccag tccttcgccg
     7441 ccgatctggt ggtgggcacc gacctcaccg acgtcatcat cgaggacttt cgcgtccgcc
     7501 aggtggccgg ctaccgggac cgctaccgct tcagcctgcg cctgcgcgaa cacatcgaac
     7561 cgccggccgc cgccgacgcg gccgtcgccg acgtgaacca gggcgtggcc gccgacgccg
     7621 ccggctggag cgacagcagc ctcgctgcgg ccggcgtgct gcaggacccg gcctccctgt
     7681 ccgcggccct cgccgcccag cccggcctgc tcgacaccct ggacatggac gccctcggcg
     7741 acgccgtggc cggcaatctg gacagcctcg acgccggcca gctcgacggc atgctgggcg
     7801 ccctcgccaa cgcggatccg accaaggctg gcggcctctt cgacgccctc ggcaaggctg
     7861 gtgccctcgg ccccatgctc gccaaatacg cccaggaagg cgcggccttc ctcaagaaac
     7921 tcgacccatc caagctcagt tcgctgatgc gcgccttctc tggcgggctg gagttcctca
     7981 accagctcaa gaaggtggcc gaggccgccg gcaagctcgc aggcgacatc aaagacctgc
     8041 gcctgccgcc cgaactggac cgcatcctca agggcggccg gccatgagca gcaccgccga
     8101 cgtggtccgc gacctggcgt ccctggtgcg ggagctcggt gccctgctgg agacaaagac
     8161 cgcccgcgag attcccgcca tcgcccgtga cctgggcgcc gaggcccagc tcggccaggg
     8221 cgtggatgcg ctggcctccg tgctcggtca ggtccgtcac ggcgtcgtgc ccctgcgcag
     8281 gatggccatc ggcgccgacg ccctggtcgc cgccctgggc tgcgtcccgc ccctggtggg
     8341 cggcctcgcc cgcgccaccg acgcctccgg cgaatggctg gcgaccctgg gcctcggcct
     8401 cggggacgcg gccggcgccg cacaggccgt ctccggcccg atccgccagg tgtcggatgt
     8461 cctcgacatg ggcgaagacg tggccgaagg cgctgtcagc ctgctcgccg cgggacagtg
     8521 gcgcggtctc atcaccgccc tcgaccgcct cgatacatcc ctcaaggcgc tcaaggcgcc
     8581 gccccccacc ccgcccggcg cccccgccgg cgccctttcc ggaggatgac ccccatggcc
     8641 gataccttac ccgcccccac ttccgacggc cgcgccgaca accccttccg cgccgtcgcc
     8701 atcctgctcg acgccgtggg ccgctccctg gaggccagca agcgccagga ccaggccgcc
     8761 cgctgggtgc gcatcgcccg cgtgcgcgaa ttccgcgacg acggcatcgc caagggcgtc
     8821 atgaaagcga tggacgtgct gaccctggcc gtcgcctacc tgcagcgctt caccctcgac
     8881 gccaacgacc tgctcgtcca gggcgacgcc gccaaggccc tggtggaggt ctcggccgaa
     8941 ttcatcaaga ccgccaccag caaggagttc atcaactccc tcgaaatggc cgtcggtcag
     9001 gacccgagcc ccgattcgcc catcccggac gtggccaaca tcatcgacaa aatcgtgcag
     9061 atcgccgaca aggtgcccga gccggacgac ctcaaggtga tcggcgcggc cctgtggcgc
     9121 ctgctgggca tcgaacaggc cagcctcgac gaggccaagc tgggcgccac caccgagagc
     9181 cacatcgtcg tggagcgcac cggcaagctg cgcctgctcc agttcgcctt cgaccagaac
     9241 ttccgcctgc agaacttcgg caagggcgcc ggcgtgtcct ccctcgacgt gaaccgcctg
     9301 ggcggccgcc gcctgtggga agccggcgcc gacaagctgc cgtccaagtc cgtgggcaag
     9361 tacggtgacg cgccggacat cgagaccgtg tgggagctgg atttctccac cgacaaggcg
     9421 ggcgccgacg tgcaggacgc caactccatc ctcgaagccc tcggctacgc cgacccggcg
     9481 gtaccggacc gcaaggtctt cggcgccgaa ttcgccggcc gcctgcgcac cttccagcag
     9541 atcaacgaga ttcccctgag cggcaagctc gacaacgcca ccctcaaccg tctgctgcac
     9601 ctggacttcg acgccaagaa cctgaagcgc gccaagcgct tcaaggccga cgccctgccc
     9661 aagggcttcg atccgctgaa gaagtgagcg accgccgacc tcatcgcccg tcctttcgcc
     9721 tgccgccatg agcgacccga tcgacatcat ccgcgccgtc gtccgcgacg agctgcgcag
     9781 cctgcgcctg ggcgacctgg cggtggtcac cagcatcttc ccccacacgg gcgacgatga
     9841 cggccacaac cacgaatgct cggtcaagct gcgcgaaggc gggctcgaac tgcgcaaggt
     9901 gcccatcgcc acgccccacg tgggcatggt cggcgcgccg cgggtgggcg atctggtcct
     9961 cgtcagctac gtgggcggcg accccaaccg ggcggtggtg atcggccggc tgtattccga
    10021 aaagatccac ccgcccctcc acaaggagga cgagtggcac gccttcgcca aggccgacgg
    10081 ggtcgcccgc tacaccattg acggcgagga atcctgcatc gtcacggccg gcaagaccat
    10141 cgtcacggtg aagaaggacg gcaacgtgga gatccagggc gaagccgacc tggccatcca
    10201 ggtcaagggc aaggtcacga tccaatgcac cgacttcagc ctcgacgcct ccggcaacgt
    10261 ggatctgggc cagagcggcg ccggcgtgat caccgagcag tcccacaagt gttactttac
    10321 cggcaaggcc ctggtgccct ccggcaccgt caaggccaaa ggctagg
//
LOCUS       DAOQMM010000122        10351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3037_length_10351_cov_48.274087, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000122 DAOQMM010000000
VERSION     DAOQMM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_10351_cov_48.274087"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..663
                     /locus_tag="PLH85_15230"
     CDS             388..663
                     /locus_tag="PLH85_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65970.1"
                     /translation="MFEDDGMHTPADDELVVTPYALLRKGKDRKWDGWYDLARPNGAQ
                     VRTFVRVPSRDGFDDPHMACMAAEVLARFDLDGALKRTSSRRKPEVS"
     gene            688..960
                     /locus_tag="PLH85_15235"
     CDS             688..960
                     /locus_tag="PLH85_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNB65971.1"
                     /translation="MTPRLALTVAVLALFECSVPASAAVYKCDEGGRVVYMDRPCLYA
                     PVEAKSAVRTVGVPSGEGERRERVQPVRTLPADESAILQARPLVVR"
     gene            complement(1073..1732)
                     /locus_tag="PLH85_15240"
     CDS             complement(1073..1732)
                     /locus_tag="PLH85_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNB65972.1"
                     /translation="MKNLRTALIGSALALGTLFAVQGCIPVIVGGTGVAVAMATDRRT
                     SGTYVDDESIEWKASNRISEKLKDNVHANVTSFNRKVLLTGEAFNETSRDEAGRIAAG
                     VENVKEVVNELRIAPTSSLSARNNDAYISSKIKTRFLDRTDFHVQHVKVVTEAQTVYL
                     LGLVTRKEADAATQIARTTQGVNKVVRVFELISDEDAARLDSATGGSNENPVKSPNPR
                     S"
     gene            complement(1729..2322)
                     /locus_tag="PLH85_15245"
     CDS             complement(1729..2322)
                     /locus_tag="PLH85_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HNB65973.1"
                     /translation="MDLVARIAQQFNDSAQTKLAALEFMAAPIAAAIETMTHCLMANG
                     KILACGNGGSAADAQHFAAELLNRFEMERPPLAAMALTTDTSTLTSIANDYAYDQVFS
                     KQVRALGQPGDVLLAISTSGNSPNVIEAIHAAREREMRVVALTGKGGGRMTGLLGPDD
                     VHLCVPADRTARIQEVHLLTLHCLCDGIDCLLLGVEE"
     gene            complement(2646..3029)
                     /locus_tag="PLH85_15250"
     CDS             complement(2646..3029)
                     /locus_tag="PLH85_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNB65974.1"
                     /translation="MRSRRYRARVAGLALVLCTGSSLAQQVRILVQNSPLAGFQYYSG
                     KILWDEMREGDPLVLVREPDNRHDTAAIRVEWRGVQLGHLPRAENAALAAEMDRGVKV
                     EGRIGRLTKDPNPWKRLRVEVYVRP"
     gene            complement(3019..3372)
                     /locus_tag="PLH85_15255"
     CDS             complement(3019..3372)
                     /locus_tag="PLH85_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNB65975.1"
                     /translation="MQRGAAAEALAAQHLEGHGLRILARNVRCRGGEVDLIALDHDTV
                     VFVEVRLRRNGRFGGAAASITATKQQRVILAAQHWLAGAGRTHARRPCRFDAVLLDAL
                     SSAHIAWLKGAFDAE"
     gene            3489..4316
                     /gene="rsmI"
                     /locus_tag="PLH85_15260"
     CDS             3489..4316
                     /gene="rsmI"
                     /locus_tag="PLH85_15260"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNB65976.1"
                     /translation="MYVVATPLGNLQDLGLRALAVLKAVDAVAAEDTRHSQRLLDAFG
                     IATKLVALHEHNEQSAVGQVIRLLEAGQHVALVTDAGTPAVSDPGARAVARVQEAGFP
                     VVPVPGPCAAVTALSASGLADGAFHFHGFLPVKSAARRAALEALRGVPGTLVFYEAPH
                     RIAECLDDLAAVLEAGRQIVVARELTKLFEQIVRMPLADAPAWLAADPNRARGEFVLL
                     VSAAPPAEGLSPEAERVLGLLLAELPVKQAAKLAAAITGASKNALYERALALRDTAL"
     gene            complement(4329..5471)
                     /locus_tag="PLH85_15265"
     CDS             complement(4329..5471)
                     /locus_tag="PLH85_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB65977.1"
                     /translation="MMDRWSGKWLVMLAVVLGIAGGAGTAGAETVVIGQCAPLTGSLG
                     STGKQMALGVRIAVAAANAAGGVGGHRLKHVVKDDGYQTSETVRLTRELIERDKAAAL
                     IGYAGTGNIVELLKQGVLADGTTALIAPYTGGEPLRSPYNPWIFHIRASYGDETEAMV
                     RQFVTTGIKRIAVFYQNDPFGQAGLAGVERALARFDLKVASSGSYEKNSEDVASAVQD
                     IAKGNPQAVIMIGVVRPAAAFVKAYRSVAPGTLLFSISVINGRELFQIAGGDFARGVG
                     ITQVMPSPFDGVSRIARDYADALRAYAPGEEPSYTSFEEYVGARVLIEAIRRVKGPPT
                     PLAVAQSLESLDLDVGGFKVKFGPGNRVGSRHVDVTLIGRDGSHIH"
     gene            complement(5593..6927)
                     /locus_tag="PLH85_15270"
     CDS             complement(5593..6927)
                     /locus_tag="PLH85_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB65978.1"
                     /translation="MSQPLRVLIIEDDPDVRLGCEQALMLADMAVDGVGSAEEGLARL
                     GPGFPGVVVTDMRLPRADGMEVLRRARALDADLPVIIITGHGDVTLAVEAMRGGAYDF
                     IQKPFSPEMLVDVVRRALDKRALTLEVLDLRRRLAERDGIDAKLIGRSPQMARVRQLV
                     ADVAGTRVDVLIRGETGTGKELVAQCLHELSPRKGGPFVALNCGGLPDTLLDSELFGH
                     EAGAFTGAQKRRIGKIEYANKGTLFLDEVESMPLGVQIKLLRVLQERVVERLGSNQQQ
                     PVDCRVVAATKDDLKARAEQHSFRADLYYRLNVVTIDLPPLRERREDIPLLLEHFMLG
                     AAQRHDRPLPSVPPERMRRLMAHPWPGNVRELRNMADCLVLGVGQERLDGAPDTVPPS
                     LAEAVDSFERSLIAAELRRQGGNLSRSAEALRIAKTTLHDKIRKHGLQGGDT"
     gene            complement(6924..8870)
                     /locus_tag="PLH85_15275"
     CDS             complement(6924..8870)
                     /locus_tag="PLH85_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB65979.1"
                     /translation="MSPSHPPPPAEASPAEAPARSEAGTPPSRRVSTHPLLVAAFFIV
                     TGLIGLSAWRASEYQGIQTLRADSGHRVDLFAAAVDGIVNRYAHIPATIQLNEDVLQL
                     LRTPRHRPAVSAGNRFLQQLNSHVGSIAIFVLDERGIVLAASNWDHPDDSFVGEDLSF
                     RNYFLTALSGRTGHHFAIGITRGEPGYFVSHPIRDGDRVVGVAVIKISLEQLDRAWHL
                     LGVPALIADRNGVVILSSVPAWRYTALSPLPLDTRVEVRLSRLYNEAAIGAFPVTLPA
                     QLDPEGQIVETGAHLGEPGHRAGLTARYLVHGRDLPDLGWRLLIFSDLRPVQTQALGH
                     AALAAVATGFILLLLLVLAQRRRILRQKLEAKTLLEQANAQLESKVARRTRALTETNA
                     RLRKEVAERQQAEQTLRAAQDELVQAAKLAVLGQLAAGITHELAQPLGAMRTLSGNAV
                     AFMKRGDLDTVEKNLGIIARLADQMGRIITPLKTFARKSPAIPAATDLAHAVSAALFL
                     LDPRLAKAGVTVINRCEAGRDIAWCDQNRLEQVLINLIRNAADAMADAPVRELLIEAG
                     PDDAGGLALRIADSGCGLPDPAAGRLFEPFFTTKPAGEGLGLGLAISRDIVREFGGEL
                     SADNRPEGGARFTLHLPAAPKDAP"
     gene            complement(8988..10271)
                     /locus_tag="PLH85_15280"
     CDS             complement(8988..10271)
                     /locus_tag="PLH85_15280"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNB65980.1"
                     /translation="MTTLSLPSYIDPAHTGPWGMFLEQLDRVAPHLGPLAEWLDTLRH
                     PKRCLVVDVPVRMDDGRIRHFEGFRVQHNLSRGPGKGGVRFHPDVTLSEVMALSAWMT
                     VKNAAVNLPFGGAKGGVRVDPKTLSTPELERLTRRYTSEIGTLIGPDRDIPAPDVNTN
                     EQIMAWMMDTYSINQGSTQTGVVTGKPVSMGGSLGRRDATGRGCFVVAREALKRLGRP
                     LTGARVAIQGFGNVGNAAARIFHDAGAKIVAIQDVAGTLHHPGGIDPHALLRFLDGDP
                     RRLTAYPEAGVIDNADFWSTDCEILLPAALEHQITADNAPRIRAKLIVEGANGPTTPQ
                     AEDILDAAGCTVLPDVLANAGGVTVSYFEWVQNASSFFWTEKDIHDRLDRILSEAFQA
                     IWELAAARRVNLRTATYIMACSRVLQARAQRGLYP"
BASE COUNT         1421 a         3289 c         3922 g         1719 t
ORIGIN      
        1 ggcatccgcg tggcgcccgg cacgatgttt tccaattccg gacgtttcga ccatttcctg
       61 cggatctgct gcggtgcggc ctattcgccg tccatcgagg cggcattgcg tacgctcgcc
      121 cggattgtct cggccggctg acccggcgca ccggggccag cgccctcgcg aagcgccggc
      181 aagatctgca agccgggcgg cgtgtcgtcg cctgtatgcc tgctgtatga aatccccata
      241 ttgagatttc tttgtaaatc ttgattaaat aagtggtttt ctgtctctcc gaaccctccg
      301 tcgtgcgttg tcgaaacacg catgtctcta tacttggccg cctcgcgttc cccgcatcag
      361 gggatgggcg gatcggaaat caggtgcttg ttcgaggacg atggaatgca caccccggca
      421 gacgatgagc tcgtggtgac gccgtatgcc ctcctccgca aggggaagga tcggaagtgg
      481 gatggttggt acgatctggc gcggccaaac ggggcgcaag tccgtacctt tgtccgcgtc
      541 ccgtcccgtg atggttttga cgatcctcac atggcctgca tggccgctga agtcctggcc
      601 cgcttcgatc tcgacggggc cctgaaacga acgtcctccc ggcgcaagcc ggaggtttcc
      661 tgacctgctg ccgccctgga tggcctcatg accccgcgcc ttgccctgac tgttgccgta
      721 cttgccctgt tcgagtgttc cgtacccgcg tccgctgcgg tgtacaagtg cgacgaaggg
      781 gggcgcgtcg tgtatatgga ccggccttgc ctgtatgcgc cggtggaggc caagtccgcc
      841 gtccggacgg tgggcgtgcc gtccggcgag ggcgagcggc gcgaacgggt gcagccggta
      901 cgtaccctgc ccgcggacga atcggccatc ctgcaggcgc gtccgctggt cgtccgctga
      961 aaaggcggcg gggcgtgccg acgcaggcaa ggccacgcac gcaccaacgc accaatgaaa
     1021 acaccccgcg ccgggcgggg tgttgcggga ctgccggccg gatcggcgct gctcaggaac
     1081 ggggattcgg tgacttgacc ggattctcgt tgctgccgcc ggtggcgctg tcgaggcggg
     1141 cggcgtcttc gtcgctgatc agttcaaaca cccgcacgac cttgttgacg ccctgagtgg
     1201 tgcgggcgat ctgggtggcg gcatcggctt ccttgcgggt gaccagaccc agcaggtaga
     1261 cggtctgggc ctcggtgacc accttgacgt gctggacgtg gaagtcggtc cggtcgagga
     1321 agcgggtctt gatcttggag ctgatgtagg cgtcgttgtt gcgcgccgac aggctgctgg
     1381 tcggggcgat gcgcagctcg tttaccacct ccttcacgtt ttcgacaccg gccgcgatgc
     1441 ggcccgcttc gtcgcggctg gtctcgttga aggcctcacc ggtgagcagc actttgcggt
     1501 tgaaggacgt gacgttggcg tggacgttgt ccttgagctt ttcggagatg cggttggagg
     1561 ccttccattc gatggactcg tcgtccacgt aggtgccgga agtgcgtcgg tcggtggcca
     1621 tggcgactgc cacgccggta ccaccgacga tcaccgggat gcagccctgg acggcgaaaa
     1681 gggtgccgag ggcgagggcc gacccgatca gggcggtgcg caggttcttc attcttcgac
     1741 tccgagaagc agacaatcaa taccgtcgca cagacagtgc agggtgagca ggtggacttc
     1801 ctggatgcgc gcggtgcggt cggcgggcac gcacagatgc acgtcgtccg ggccgagcag
     1861 accggtcatc cggccgccgc ccttgccggt gagggcgacc acacgcatct cccgttcgcg
     1921 ggcggcgtgg atcgcctcga tgacgttggg cgaattgccg ctggtggaaa tcgccagcag
     1981 cacgtcgccg ggctggccga gggcgcgcac ctgcttggag aagacctgat cgtaggcgta
     2041 gtcgttggcg atggaggtga gcgtcgaggt gtcggtggtg agggccatcg ccgccagcgg
     2101 tgggcgctcc atctcgaagc ggttgaggag ctccgccgcg aagtgctggg cgtcggccgc
     2161 cgatccgccg ttgccgcagg caaggatctt gccgttggcc atcaggcagt gggtcatggt
     2221 ttcgatggcc gcggcaatgg gagcggccat gaattcgagg gcggccagct tggtctgggc
     2281 gctgtcgttg aactgctggg cgatgcgggc gacgagatcc atggggggct ccggtagggt
     2341 gcgcggattc tagcatcggg gcgcggcgct tcgtggggag gaagaaatct gcttacaaag
     2401 ggcgtcgagg gagaggcgcg gcggtggcgg cgcgggcagg cgccgtcgtg ccggcgtgcc
     2461 ccgaggcgcc gtggcatctg ggtgggcgag cgggccgctg cgagggtctg cccggcctgg
     2521 gctccgcggg cgtggcggcg ttcgccacgc ggctttcagg ggcggtgcgt gaggcggctg
     2581 gcgccagaag ggcgcgggcc tgtcgaggcc gcggaggccc gttcaaggta tccggttggc
     2641 ggttgtcatg gccgcacata cacctccacg cggaggcgct tccacggatt cggatccttc
     2701 gtcaggcgtc cgatgcgacc ttccaccttg acgccccggt ccatctcggc agcgagggcg
     2761 gcgttttcgg cgcggggcag gtgaccgagc tggacgccgc gccattctac gcggatcgcg
     2821 gcggtgtcgt ggcggttgtc gggctcgcgg acgaggacca gcgggtcgcc ctcgcgcatc
     2881 tcgtcccaca ggatcttgcc gctgtaatac tggaagccgg ccaggggcga gttctgcacg
     2941 agaatgcgca cctgctgggc gaggctgctg ccggtgcaga ggacgagcgc gaggcctgcg
     3001 acgcgcgcgc ggtaccgtct actccgcatc gaaggcgccc ttgagccacg cgatgtgcgc
     3061 gctggagagg gcgtccagca gcaccgcgtc gaagcggcag ggccgcctgg cgtgggtgcg
     3121 cccggcgccg gccagccagt gctgggcggc caggatcacg cgctgctgct tggtcgcggt
     3181 gatgctcgcc gcggccccgc cgaagcgccc attgcggcgc aggcggactt cgacgaacac
     3241 caccgtgtcg tgatccaggg cgatcagatc cacctcgccg ccgcggcagc gtacgttgcg
     3301 cgccaggatg cgcaggccgt ggccttccag atgctgggcc gcgagggctt cggctgccgc
     3361 gccgcgctgc aggtgagcgg cttgcgccgg ctcctcggcc ccgccacaat cgggctcccc
     3421 cgcctttcgc caccatgtcc gccatgcttc ccgcaccttc cgaatctccc ctgtcaagga
     3481 cgccgtcatt gtatgtggtt gccacgcccc tcgggaacct ccaggacttg ggcctgcggg
     3541 cgctggcggt gctgaaggct gtggatgcgg tggcggccga ggacacgcgc cacagccagc
     3601 gcctgctgga tgccttcggg atcgccacga agctggtggc gctccacgag cacaacgagc
     3661 agtccgccgt cggccaggtg atccggctgc tggaggccgg gcagcacgtc gcgctggtca
     3721 ccgatgcggg gacgccggcc gtgtccgacc cgggcgcgcg ggcggtggcg cgggtgcagg
     3781 aggcgggctt cccggtggtg ccggtgccgg ggccctgcgc tgcggtgacc gccctgtcgg
     3841 cctccggcct ggcggacggg gccttccatt tccacggttt tctgccggtc aagtcggcgg
     3901 cgcggcgggc ggccctcgag gcgctgcgcg gtgtccccgg caccctggtg ttctacgagg
     3961 cgccccatcg catcgccgaa tgtctggacg atctggcggc cgtgctcgag gcgggccgcc
     4021 agatcgtggt ggcgcgggag ctgaccaagc tcttcgagca gatcgtgcgc atgccgctgg
     4081 ccgacgcgcc ggcctggctg gcggcggatc cgaaccgggc gcggggcgag ttcgtcctgc
     4141 tggtctccgc agcgccgccg gcggaaggcc tgtcgcccga ggcggagcgg gtgctgggcc
     4201 tgcttctggc cgagctgccg gtgaagcagg cagccaagct ggccgcggcc atcacagggg
     4261 cttcgaagaa tgccctgtac gaacgggccc tggccctgcg tgacacggcc ctgtgagttc
     4321 ggcggccctc aatggatgtg actgccgtcg cggccgatga gggtgacgtc cacatggcgt
     4381 gagccgaccc ggttgccggg gccgaacttc accttgaagc cgcccacgtc caggtcgagg
     4441 ctttcgaggg attgggcgac ggccagcggt gtgggcgggc ccttgacgcg gcggatggcc
     4501 tcgatgagca cccgtgcgcc gacgtattcc tcaaagctgg tataggacgg ctcctcgccc
     4561 ggcgcatagg cccgcagggc gtcggcatag tcgcgggcga tccggctgac gccgtcgaag
     4621 ggcgagggca tgacctgggt gatgcccaca ccccgggcaa aatcgccgcc ggcgatctgg
     4681 aacagttcgc gcccgttgat caccgagatg ctgaacagca gggtgccggg cgcgacactg
     4741 cggtaggcct tgacgaaggc ggcggcgggg cgcaccacgc cgatcatgat gaccgcctgc
     4801 gggttgccct tggcgatgtc ctggacggca ctggccacgt cttcgctgtt cttctcgtag
     4861 ctgccgctgc ttgccacctt caggtcgaac ctggccaggg cccgctccac accggccagg
     4921 ccggcctggc cgaaggggtc gttctggtag aacacggcga tgcgcttgat gccggtggtg
     4981 acgaactggc gcaccatcgc ctcggtttcg tcgccgtagc tggcgcggat gtggaagatc
     5041 cacgggttgt aggggctgcg caggggctcg ccgccggtgt agggggcgat cagcgcggtg
     5101 gtgccgtcgg cgagcacgcc ctgcttgagc aattccacga tgttgccggt gccggcgtag
     5161 ccgatcagcg cggcggcctt gtcccgctcg atcagttcgc gggtcaggcg gacggtttca
     5221 gacgtctggt agccgtcgtc cttgaccacg tgtttgagcc ggtggccgcc cacgccgccg
     5281 gccgcattgg ccgccgccac ggcgatgcgc acgccgagcg ccatctgctt gccggtactg
     5341 cccaggctcc ccgtgagggg cgcgcactgg ccgatcacaa ccgtttccgc accggccgtg
     5401 cccgctccgc cggcgatccc cagcaccacg gccagcatga ccagccattt tccgctccac
     5461 ctgtccatca tcgcgtcccc cccgcaacat tgtgtcaatg ttgcggaagt gtgcatgaat
     5521 catggtgcac tgcatatgac gcgtgccact gccttcgagt gaactattac cagcccgcgg
     5581 gcagcgtgcg catcaggtgt ctccgccctg caggccgtgt ttgcggatct tgtcatggag
     5641 ggtggtcttg gcgatgcgca gggcttcggc gctgcggctc aggttgccgc cctggcgacg
     5701 cagttcggcg gcgatgaggc tgcgctcgaa gctgtccacc gcctcggcca gggacggtgg
     5761 gaccgtgtcg ggcgcgccgt cgagccgctc ctgaccgacg cccagaacga ggcaatcggc
     5821 catgttgcgc agctcgcgca cgttgccggg ccaggggtgg gccatcaggc ggcgcatgcg
     5881 ttcggggggc acgctgggca gggggcggtc gtgacgctgg gcggcgccga gcatgaagtg
     5941 ttcgagcagc agggggatgt cctcgcgccg ttcccgcagg ggcggcaggt cgatggtcac
     6001 cacgttgagg cggtagtaca ggtcggcgcg gaagctgtgc tgttcggcgc gggccttgag
     6061 gtcgtccttg gtggcggcca ccacgcggca atccacgggc tgctgctggt tggagccgag
     6121 acgctcgacc acccgttcct ggagcacgcg caggagcttg atctgcacgc ccaggggcat
     6181 gctctccacc tcgtcgagga agagggtgcc cttgttggcg tattcgatct tgccgatgcg
     6241 gcgcttctgg gcgccggtga aggcgccagc ctcgtggccg aagagctcgc tgtcgagcag
     6301 ggtgtcgggc aggccgccgc agttgagggc gacgaagggg ccgcccttgc gcggcgacag
     6361 ttcgtgcagg cactgggcca cgagttcctt gccggtgccg gtttcgccgc ggatcagcac
     6421 gtccacgcgg gtgccggcca cgtcggcgac gagctgacgc acccgcgcca tctgcggcga
     6481 gcggccgatg agcttggcgt cgatgccgtc ccgctcggcg aggcggcggc gcaggtccag
     6541 cacctccagg gtgagggcgc gcttgtcgag ggcgcggcgc acgacatcta ccagcatctc
     6601 cggggagaag ggcttctgga tgaagtcgta ggcgccgcca cgcatggcct ccacggccag
     6661 ggtgacgtcg ccgtggccgg tgatgatgat gaccggcagg tccgcgtcca gtgcgcgggc
     6721 gcggcgcagc acctccatgc cgtcggcgcg gggcaggcgc atgtcggtga cgaccacgcc
     6781 ggggaagccg ggacccaggc gggcgagccc ctcttccgcc gagccgacgc cgtccacggc
     6841 catgtcggcc agcatcaggg cctgttcgca acccaggcgc acgtccgggt cgtcttcgat
     6901 gatgagtact ctgaggggct ggctcatggg gcgtccttcg gggccgcggg gagatggagg
     6961 gtgaagcggg cgccgccttc cgggcggttg tcggcgctca gctcgccgcc gaactcgcgc
     7021 acgatgtcgc gggagatggc cagcccgagc cccagtccct cacccgcggg cttggtggtg
     7081 aagaagggct cgaagaggcg cccggcggcc gggtcgggca ggccgcagcc gctgtcggcg
     7141 atgcgcaggg cgaggccgcc ggcgtcatcc gggccggcct cgatgaggag ttcgcgcact
     7201 ggcgcgtcgg ccatggcgtc ggcggcgttg cggatgaggt tgatgaggac ctgctccagg
     7261 cggttctggt cgcaccaggc gatgtcgcgg ccggcctcgc agcggttgat gacggtgacg
     7321 ccggccttgg ccaggcgcgg gtcgaggagg aacagggcgg cgctcaccgc gtgggccagg
     7381 tcggtggcgg cgggaatggc cggcgacttg cgggcgaagg tcttgagggg cgtgatgatg
     7441 cggcccatct ggtcggccag gcgggcgatg atgcccaggt tcttctcgac ggtgtccagg
     7501 tcaccgcgct tcatgaaggc cacggcgttg cccgacaggg tgcgcatggc gcccaggggc
     7561 tgggcgagtt cgtgggtgat gccggcggcg agctggccca gcaccgccag cttggcggcc
     7621 tgcaccagct cgtcctgggc ggcgcgcagg gtctgttcgg cctgctggcg ttcggccact
     7681 tccttgcgca ggcgggcgtt ggtctcggtg agggcgcggg tgcggcgggc caccttgctc
     7741 tccagctggg cgttggcctg ttccagcagg gtcttggcct ccagcttctg gcgcaggatg
     7801 cggcggcgct gggccaggac gagcaagagc agcaggatga agccggtggc cactgcggcg
     7861 agggctgcat gacccagggc ctgggtctgc accgggcgca ggtccgagaa gatcagcagg
     7921 cgccagccca ggtcggggag gtcgcggccg tggaccagat agcgcgccgt gagtccggcc
     7981 cgatggccgg gttcgcccag gtgggcgccg gtctcgacga tctgcccctc ggggtcgagc
     8041 tgggcgggga gcgtcaccgg aaaggcgccg atggccgctt cgttgtagag gcgcgacagc
     8101 cggacctcga cccgcgtgtc caggggcagc gggctgaggg ccgtgtagcg ccaggccggc
     8161 accgagctca ggatcaccac cccgttgcgg tcggcgatga gggccggcac gcccagcaga
     8221 tgccaggccc ggtcaagctg ttcgaggctg atcttgatga ccgccacgcc caccacgcgg
     8281 tcaccgtcgc ggatcgggtg ggacacgaaa tagcccggct cgccgcgggt gatgccgatg
     8341 gcgaagtggt ggccggtgcg gccggagagg gcggtgagga agtagttgcg aaaggacagg
     8401 tcctccccga cgaagctgtc atcggggtgg tcccagttgc tggcggcgag gacaatgcca
     8461 cgctcgtcca gcacgaagat ggcgatgctg cccacgtggc tgttgagctg ctgcaggaag
     8521 cggttgccgg cgctgaccgc cggacggtgg cggggcgtgc gcagcagctg caggacgtcc
     8581 tcgttgagct ggatcgtggc cgggatgtgg gcgtagcggt tgacgatgcc gtccacggcg
     8641 gcggcgaaga gatccacccg gtggccggag tcggcgcgca gggtctggat gccctgatat
     8701 tcgctggccc gccaggcgct caggccgatg aggcccgtga cgatgaagaa ggccgccacc
     8761 agcagggggt gggtcgacac gcggcgggac ggcggcgtgc cggcttcgct gcgggcaggc
     8821 gcttcggcag gggacgcctc cgctgggggc ggcgggtggg atgggctcat ggacgggcgg
     8881 caatcgggcg cggtgggcgc tgcgggtggc gctgcgtcga gcatagcgca tccgtcggcc
     8941 cgcagccggg cgctgcgtcc cggccgggac gcaggccggc gcggcgctca gggatacagc
     9001 ccccgctggg cacgggcctg gagcacgcgg gaacaggcca tgatgtaggt ggcggtgcgc
     9061 agattcacgc ggcgcgcggc cgccagctcc cagatggcct ggaaggcttc cgacaggatg
     9121 cggtcgagcc ggtcgtggat gtccttctcg gtccagaaga agctcgacgc gttctgcacc
     9181 cactcgaagt agctcaccgt caccccgccg gcgttggcca gcacgtcggg cagcaccgtg
     9241 cagccggctg cgtcgaggat gtcctcggcc tggggcgtgg tcgggccgtt ggcgccttcg
     9301 acgatgagct ttgcacggat gcggggggcg ttgtcggcgg tgatctggtg ttcgagggcg
     9361 gcgggcagca ggatctcgca gtcggtgctc cagaagtcgg cgttgtcgat gacgcccgcc
     9421 tccgggtagg cggtgaggcg ccgcgggtcg ccgtcgagaa agcgcagcag ggcgtggggg
     9481 tcgatgccgc ccgggtggtg gagcgttccg gccacgtcct ggatcgcgac gatcttcgcg
     9541 ccggcgtcat ggaagatccg tgccgcggcg ttgcccacgt tgccgaagcc ctggatcgcc
     9601 acccgcgcac cggtgagggg ccggccgagg cgcttgaggg cttcccgcgc caccacgaag
     9661 cagccacgcc cggtggcgtc gcgtcgcccc aggctgcccc ccatggagac cggcttgccg
     9721 gtgacgacgc cggtctgggt gctgccctgg ttgatggagt aggtgtccat catccaggcc
     9781 atgatctgct cgttggtgtt cacgtccggc gccggaatgt cccggtccgg gccgatgagg
     9841 gtgccgatct cgctggtgta gcggcgggtc aggcgctcca gctccggcgt ggacagggtc
     9901 ttcggatcga cccgcacacc gcccttggcg ccgccgaagg gcaggttgac cgccgcgttc
     9961 ttgacggtca tccaggctga cagggccatg acctctgaca gggtcacgtc cgggtggaag
    10021 cgcacgccgc ccttgcccgg tccgcgggac aggttgtgct gcacgcggaa gccttcaaag
    10081 tggcggatgc ggccgtcgtc catgcggacc ggtacatcca ccaccaggca gcgcttggga
    10141 tggcgcaggg tgtcgagcca ctccgccagc gggcccaggt ggggcgccac acggtcgagc
    10201 tgctccagga acatgcccca cgggccggtg tgggccgggt cgatgtagga gggcagggaa
    10261 agtgtcgtca tggggggctc cgcggttcag tggttgagga tcttcgccag gaactgctgg
    10321 gcccgttccg acctggggcg ctcgaagaag g
//
LOCUS       DAOQMM010000123        10249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3073_length_10249_cov_55.610065, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000123 DAOQMM010000000
VERSION     DAOQMM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3073_length_10249_cov_55.610065"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="PLH85_15285"
     CDS             complement(<1..95)
                     /locus_tag="PLH85_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB65981.1"
                     /translation="MPQYRSRTSTAGRNMAGARALWRATGMKDGD"
     gene            457..1224
                     /locus_tag="PLH85_15290"
     CDS             457..1224
                     /locus_tag="PLH85_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB65982.1"
                     /translation="MSDYILETAGLTKEFKGFTAVSNVDLKVRRGHIHALIGPNGAGK
                     TTVFNLLTKFLPPTRGTIHFDGRDITREAPAVTARRGLVRSFQISATFPHLTVMENVR
                     IGLQRKLGTEFHFWRSEKSLDVLNDRAMELLEAVDLTRFADTVTAEMPYGRKRALEIA
                     TTLALEPAMMLLDEPTQGMGHEDIGRIVELIRKVSVDRTILMVEHNLSVVSHLCDRIT
                     VLQRGSILAEGPYEDVSKNPQVLEAYVGSSDMNDAHH"
     gene            1238..1960
                     /locus_tag="PLH85_15295"
     CDS             1238..1960
                     /locus_tag="PLH85_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB65983.1"
                     /translation="MTSAFDPKPEMLRVRDLHAYYGESHILHGIDFSVGRGECVTLLG
                     RNGAGRTTTLKALLGLVGRRTGSIMLNGKEIVAEPTHRIAGYGVGYVPEERAIFASLS
                     AEENLLLPPQVASGGMTVDEIYAMFPNLRERRSSPGTKLSGGEQQMLAMARVLRTGAK
                     LLLLDEITEGLAPVIVKKLGEVIVELKSRGFTIILVEQNFRFAAPLADRHYVLEHGEI
                     LATIPQEELPGRMDWLHQTLGV"
     gene            1995..3212
                     /locus_tag="PLH85_15300"
     CDS             1995..3212
                     /locus_tag="PLH85_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB65984.1"
                     /translation="MTTSKKLLTAACTAALATLSAGAHAQISGGVVKIGVLTDLSGTY
                     SDLAGSGAVLATKMAVEDFIAKEKPTFKIEVVSADHQNKADIASNKAREWFEREGVDV
                     ASELVTTSVALAVMKIAKEKDRIALMSGPASTPITNEQCNDVTVHYTYDTYALANGTA
                     KAVTKQGGKTWFFLTADYAFGQALEKDASAVVTANGGQVLGSVRHPFPGSDFSSFLLK
                     AQASGAQVIGLANAGADTTNSIKQAAEFGITPKQSLAGLLMFITDIHALGLKATQGMY
                     LTEGFYWDLNDQTREWSRRFMGAQKKMPTMVQAGQYSSVYHYLKAVKAAGTDDTKKVM
                     EMMKKTPINDFFAKNGRIREDGRMVHDMYLMQVKKPAESKYPWDYYNVRQVIPADEAF
                     QPLSLSRCPLVKK"
     gene            3269..4150
                     /locus_tag="PLH85_15305"
     CDS             3269..4150
                     /locus_tag="PLH85_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB65985.1"
                     /translation="MEIFGVPTALLGSQLLVGLINGSFYAILSLGLAIIFGLLNIINF
                     SHGAQYMLGAFVAWIALTRFEINYWVMLIAAPLLVGAFGVVLERTMLRKLYKLDHLYG
                     LLLTFGLALIIEGVFRDQFGISGESYEVPEALAGGINLGFMFLPIYRAWVIVASLTVC
                     FGTWFMIEKTKLGAYLRAGTENPQIVQALGINVPLLITFTYGYGVALAAFAGVLAAPI
                     FQVNPLMGSNLIIVVFAVVVIGGMGSIMGSILTGIGLGLIEGLTRVFYPEGSAVVVFV
                     IMVIVLLVRPAGLFGKA"
     gene            4175..5131
                     /locus_tag="PLH85_15310"
     CDS             4175..5131
                     /locus_tag="PLH85_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB65986.1"
                     /translation="MNASRQPTALIKPEVLYTALFIVGLIAPFAVYPTFLMKLFCFAL
                     FASAFNLLLGFAGLLSFGHAAFLGSAGYVCGMMVRDVGVTPEVGILAGTAAAGLLGWL
                     FGVLAIRRTGIYFAMITLALAQMVYFIVLQVKATGGEDGLQGVPRGHLFGFIDLTDNL
                     AMYYLVYGVFALGFFIIHRTIHSPFGQILKAIRENEPRAISLGYDVSRFKLIAFVISA
                     SLAGLAGATKTLVFQLVSLSDVHWHMSGEVVLMTLLGGLGTILGPSVGSGVIVTLQNE
                     LADKVGSWVTVIMGAIFVLCVLAFRRGLVGEIQALIHKAGIR"
     gene            complement(5177..6019)
                     /locus_tag="PLH85_15315"
     CDS             complement(5177..6019)
                     /locus_tag="PLH85_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB65987.1"
                     /translation="MQHFIADDGECLHVQVSGEGPPVILLHGWTSSHRDWNPFVDVLS
                     ADHHVLRWDARGHATHALQAATAPTVQRMARDLANLLDHWHLERAVLVGHSMGALTLW
                     QYLQDFGSDRVAGVVIVDQSPRLVTDATWRNGIYGDFDTARNAAFIAELEADFGEAVL
                     RLTAFGRNGEVRRRYTDQPESFDNARRWLGRLHHAALIACWRTLTAADYRAVLPAIDV
                     PALLVYGAESNFYSRETAEWVAGAIPDARLTVYPGADHSPHLHDRARFGADLDDFLAR
                     VFPR"
     gene            complement(6077..7699)
                     /locus_tag="PLH85_15320"
     CDS             complement(6077..7699)
                     /locus_tag="PLH85_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB65988.1"
                     /translation="MRRLADTPIWLRLTAAIWLMLVIAWGGMIVWETRTNRETAVAQA
                     EDFARSIHEMTMAGLTGMMITGTVGQREVFLDQIKQLSIIHDLQVLRGDAVSRQFGPG
                     TRPQPPLDADEQAVLATGQAVSRIQTDPRLGEYLRVIRPAIASDNYLGKNCIGCHLVP
                     AGTPLGLVSMKVSLDKVNEAVESFRWKSIISALVLSLPLILFVTYFIRSFVARPLADM
                     NRSLHEIAKGEGDLTRRLPVRGQDEIGQAADAFNAMLGTVASLVRRVSDSASRVTASS
                     HQLTMSASRVAEGARAQCEQSASAASEVETMASDIAHVAVSTEGVHQRSRESLRRSQD
                     GSRSLDMLVGEVGQAEASVRQMADSVGQFVESTSAITTMTQEVREIAEQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAEKSARSAGEIDAVTSRLSQQSVAVRQAIHEGLEH
                     LESSRVAVGEVSAVIVSANESVEAVGRGLDEIAGATERQRAASRRVAESIDAMAEMAR
                     ASNGAVESTADAARDLERLASELQETVARFRV"
     gene            complement(7848..8444)
                     /gene="pth"
                     /locus_tag="PLH85_15325"
     CDS             complement(7848..8444)
                     /gene="pth"
                     /locus_tag="PLH85_15325"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNB65989.1"
                     /translation="MSQTAPRLVVGLGNPGAEYSETRHNAGFWFCERLARELGASFSK
                     EARFHGLAAHARDAGVWLLMPQTFMNRSGQAVAALARFYRIPPAEILVVHDELDIPPA
                     HLRLKFGGGLGGHNGLKDITAHLGTQDYWRLRIGIGHPGDRNEVVNFVLKPPRREEAA
                     LIDDALDRALAVWPRIAAGDWNAATQRINARPPGDAKK"
     gene            complement(8476..9081)
                     /locus_tag="PLH85_15330"
     CDS             complement(8476..9081)
                     /locus_tag="PLH85_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNB65990.1"
                     /translation="MTIQFNASKREAQGSSASRRLRRAGRVPAIIYGGEVAPLQVELD
                     HNEIFHLLRNEAFHSSVLTALVDGAAQAVILRDTQWHPYKQQVLHLDFQRVDSTHKIH
                     VKVPLHFVNADIAPGVKLEGGIVSHVMTELDLECLPGDLPEFVEVDLKDLKGGASIHV
                     SHLPLPAGVKALTHGEDPVVATILAVRGGGEDEAAAETPAA"
     gene            complement(9168..10118)
                     /locus_tag="PLH85_15335"
     CDS             complement(9168..10118)
                     /locus_tag="PLH85_15335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNB65991.1"
                     /translation="MASGSLMVFTGNANPKLAADVVRRLGRSLGSATVGRFSDGEVNV
                     ELLENVRGKDVFVLQPTGAPTNDNIMELMIMVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVPITAKVVANMLQAVGVQRVLTVDLHADQIQGFFDIPVDNIYAAPVLLDDLEK
                     QKYDDLLVVSPDVGGVVRARAFAKRLECDLAIIDKRRPKANVSEVMNIIGEVDGRTCV
                     IMDDIVDTAGTLCKAAQALKENGAKRVMAYCTHAVLSGPAVSRIMDSDLDELVVTDTI
                     PLTDEARACSRIRTVSIASLLADTIVRISNEESVSSLFTE"
BASE COUNT         1770 a         3530 c         3160 g         1789 t
ORIGIN      
        1 aaatcgccgt ccttcatgcc ggtggcgcgc cacagggcgc gggcgccggc catgttgcga
       61 ccggcggtgg aggtgcggga acgatactgg ggcatttcgg gaaactccgt gatcactgac
      121 gggaagactg tcaatcctcc attctagccc cgatccggga tcgatccgcc acatcacgca
      181 gcgacgcacc tttgcggtgc gttgacggcc acggacattc gggtattccc tcatggccac
      241 cacagcaatt aagcgtctta atagacacat aaacaaaatc gcagcgcgcc cacagcgccc
      301 gcggcgcaac acggagaccc gccttcatga ccgctttcgc ctgttgctgg atgctgcttg
      361 ccgccagcct cgtcctgccc cggcaccccc gccccctgcg cccacgacgt atcgggtccg
      421 tcctgcagcc ccgctgcact cgccgctgag cccgtcatga gcgactacat cctggaaaca
      481 gctggcctga ccaaggaatt caagggattc acggccgtct cgaacgtgga cctcaaagtc
      541 cgtcgcggcc acattcacgc cctcatcggc cccaacggtg cgggcaagac cacggtcttt
      601 aacctcctca ccaagttcct gcctcccacc cgcggcacca ttcacttcga cgggcgcgac
      661 atcacccgcg aggcccccgc cgtcaccgcc cgccgaggcc ttgtacgctc cttccagatc
      721 tccgccacct tcccgcacct caccgtgatg gagaacgtgc gcatcggcct gcagcgcaag
      781 ctgggcaccg agtttcattt ctggcgctcg gaaaagagcc tggatgtcct caacgaccgg
      841 gccatggagc tcctcgaggc cgtcgatctg acccgcttcg ccgacaccgt caccgccgag
      901 atgccctacg gccgcaagcg cgccctggag atcgccacca ccctggcact ggagcccgcg
      961 atgatgctcc tcgacgaacc gacccagggc atgggccacg aggacatcgg ccgcatcgtc
     1021 gagctgatcc gcaaggtctc ggtcgaccgc acgatcctga tggtggagca caacctctcc
     1081 gtggtgtccc acctgtgcga tcgcatcacc gtcctgcaac ggggcagcat cctcgccgag
     1141 gggccttacg aggacgtctc gaagaatccc caggtgctgg aagcctatgt gggctccagc
     1201 gacatgaacg acgcccacca ctgagacccg acgcgtcatg accagcgcct tcgatcccaa
     1261 acccgagatg ctgcgcgtgc gcgacctgca cgcctactac ggcgaatccc acatcctgca
     1321 cggcatcgac ttctccgtcg gacgcggcga atgcgtcacc ctgctgggtc gcaacggcgc
     1381 cggacgcacc accaccctca aggccctcct cggcctggtg ggtcggcgca ccggctccat
     1441 catgctcaac ggcaaggaaa tcgtcgccga gccaacccac cgcatcgccg gctacggggt
     1501 gggctacgtc cccgaggagc gggccatctt cgcctccctc agcgccgagg agaacctgtt
     1561 gctgcccccc caggtggcga gcggtggcat gacggttgac gagatctacg caatgttccc
     1621 caacctgcgg gagcgccgca gcagccccgg caccaagctc tctggtggcg aacagcagat
     1681 gctcgccatg gcccgcgtgc tgcgcaccgg cgccaaactc ctgctgctgg acgaaatcac
     1741 cgagggcctg gcgccggtca tcgtcaagaa gctcggagaa gtcatcgtcg aactcaaatc
     1801 acgcggcttc accatcatcc tggtggagca gaacttccgc ttcgccgccc cgctggcgga
     1861 ccgccactac gtgctcgaac acggcgagat cctcgccacc atcccccagg aggaactgcc
     1921 cgggcggatg gactggctgc atcaaaccct gggcgtgtga cgcccgcccc ccattacaca
     1981 acaaggagag agacatgacg acctcgaaga agctgctgac cgccgcctgc actgccgccc
     2041 ttgccaccct gtctgccggc gcccatgcgc agatttccgg cggcgtggtc aagatcggcg
     2101 tcctcaccga cctgtccggc acctactccg acctggcggg atcgggcgcc gtactcgcca
     2161 ccaagatggc ggtcgaagat ttcatcgcca aggaaaagcc cacgttcaag atcgaggtcg
     2221 tcagcgccga ccatcagaac aaggccgaca tcgcctccaa caaggcccgg gaatggttcg
     2281 agcgcgaagg cgtggacgtg gcctccgaac tcgtcaccac ctccgtcgcc ctggcggtca
     2341 tgaagatcgc caaggaaaag gatcgcatcg ccctgatgag cggccccgcc tccaccccca
     2401 tcaccaacga acagtgcaac gacgtcaccg tccattacac ctacgacacc tacgccctgg
     2461 ccaacggcac cgccaaggcc gtcaccaagc aggggggcaa gacctggttc ttcctcaccg
     2521 ccgactatgc cttcggtcag gccctcgaga aggatgcgtc cgcggtggtc accgccaacg
     2581 gcggccaggt gctcggttcg gtgcgtcacc ccttcccggg ttcggatttc tcctccttcc
     2641 tgctcaaggc ccaggcctcc ggcgcccagg tgatcggcct ggccaatgcg ggtgccgaca
     2701 cgaccaactc catcaagcag gccgcagagt tcggcatcac gcccaagcag tccctggccg
     2761 gactgctgat gttcatcacc gacatccatg ccctcggcct caaggccacc cagggcatgt
     2821 acctgaccga aggtttctac tgggacctca acgatcagac ccgcgaatgg tcgcgccgct
     2881 tcatgggtgc gcagaagaag atgcccacca tggtccaggc cggccagtac tcttcggtgt
     2941 atcactacct gaaggccgtc aaggccgccg gtaccgacga caccaagaag gtcatggaaa
     3001 tgatgaagaa gacgcccatc aacgacttct tcgccaagaa cggccgcatc cgggaggacg
     3061 gacggatggt ccacgacatg tacctgatgc aggtgaagaa gcctgctgag tccaagtatc
     3121 cgtgggacta ctacaacgtc cgtcaggtga ttcctgccga cgaagccttc cagcccctgt
     3181 ccctgtcccg ctgcccgctg gtcaagaagt aatcgcaacc gccacagcgc ccggatcggc
     3241 ctgcgccggc ccgggcccgg agtgacccat ggaaatcttc ggcgtgccga ccgccctgct
     3301 gggcagccag ttgctggtgg ggctgatcaa cggctccttc tacgcgatcc tcagcctcgg
     3361 gctggcgatc atcttcggcc tgctcaacat catcaatttc tcccacggcg cccagtacat
     3421 gctcggcgcc ttcgtcgcgt ggatcgccct tacccgcttc gagatcaact actgggtgat
     3481 gctgatcgcc gcgccgctgc tggtgggcgc tttcggcgtg gtgctcgagc gcaccatgct
     3541 gcgcaaactc tacaagctgg accacctcta cggcctgctc ctcacctttg gcctggccct
     3601 catcatcgaa ggcgtgttcc gcgaccagtt cggcatctcc ggcgaatcct acgaggtgcc
     3661 ggaagccctc gcgggcggca tcaacctggg cttcatgttc ctgccgatct accgcgcctg
     3721 ggtgatcgtc gcgtccctga ccgtgtgctt cggcacctgg ttcatgatcg agaagaccaa
     3781 gctcggcgcc tacctgcgtg ccggcaccga gaatccgcag atcgtccagg cgctcggtat
     3841 caacgtgccg ctgctgatca ccttcaccta cggctacggg gtggctctgg cagccttcgc
     3901 cggcgtgctc gcggcgccga tcttccaggt caatccgctg atgggctcca acctgatcat
     3961 cgtcgtcttt gccgtggtgg tgatcggcgg catgggctcc atcatgggct cgatcctcac
     4021 cggcatcggc ctgggcctca tcgaaggcct cacccgggtg ttctacccgg aaggctcggc
     4081 ggtggtcgtc ttcgtcatca tggtcatcgt cctgctggtc cgtccggcgg gcctgttcgg
     4141 caaggcctga gcccgcccga tcgcgaggaa ttcaatgaac gcgtcccgcc aacccaccgc
     4201 cctgatcaaa cccgaggtgt tgtacaccgc gctcttcatc gtgggcctga tcgccccgtt
     4261 cgcggtgtat cccaccttcc tgatgaagct gttctgcttc gcactctttg ccagcgcctt
     4321 caacctgctg ctcggatttg ccggcctgct gtccttcggc catgcggcct tccttggcag
     4381 cgcgggctac gtgtgcggga tgatggtgcg cgacgtgggc gtcacgccgg aggtcggcat
     4441 cctggccggc actgcggccg ccggcctgct gggctggctg ttcggggtgc tggcgatccg
     4501 ccgcaccggc atctacttcg cgatgatcac cctggccctc gcccagatgg tgtatttcat
     4561 cgtcctgcag gtgaaggcca ccggcggcga ggacggcctg cagggcgtgc cccgcggcca
     4621 cctgttcggc ttcatcgatc tcaccgacaa cctggccatg tattacctgg tgtacggggt
     4681 gttcgccctc ggcttcttca tcatccaccg caccatccac tcgcccttcg gccagatcct
     4741 caaggcgatc cgcgagaacg agccgcgcgc catctccctg ggctacgacg taagccgctt
     4801 caagctcatc gctttcgtga tctcggcttc cctggccgga ctggcgggtg ccaccaagac
     4861 cctggtgttc cagctcgtgt ccctgtcgga tgtgcattgg cacatgtccg gcgaggtggt
     4921 cctgatgacc ctgctcggcg gtctcggcac catccttggc ccgtccgtcg gctcgggcgt
     4981 gatcgtcacc ctgcagaacg agctggccga caaggtggga tcgtgggtga cggtgatcat
     5041 gggggcgatc ttcgtgctct gcgtgctcgc cttccgccgc ggtctggtcg gcgagatcca
     5101 ggctctgatc cacaaggccg gcatccgctg agccgcgccc ctatggacgc cgcaacgcgc
     5161 ggcgtccccc gccccctcag cgcggaaaga cccgcgccag aaagtcgtcc agatccgccc
     5221 cgaaacgcgc ccggtcgtgc aggtggggcg aatggtcggc gccgggatac accgtcaggc
     5281 gcgcatcggg gatcgcgccg gccacccatt cggccgtctc ccggctgtag aaattgctct
     5341 cggcgccata caccagcaag gccggtacat cgatggccgg cagcaccgcc cggtagtccg
     5401 ccgcggtaag ggtccgccag cacgcaatga gcgcagcgtg atgcagacgc ccgagccagc
     5461 gccgggcgtt gtcgaagctc tcgggctggt cggtgtaacg ccggcgcacc tcgccattgc
     5521 gaccgaaggc ggtcaggcgc agcaccgctt cgccaaaatc cgcctccagt tccgcgataa
     5581 aggccgcgtt gcgggcggta tcgaaatcgc cgtagatgcc gttccgccag gtggcatccg
     5641 tcaccagccg gggcgactgg tccacgatca ccaccccggc gacgcggtcg ctgccaaagt
     5701 cctggaggta ttgccacagg gtcagcgcgc ccatggaatg accgaccagc accgcccgtt
     5761 ccaggtgcca gtgatccagc agattcgcca gatcgcgcgc catgcgttgc acggtgggcg
     5821 cggtggcggc ctggagcgca tgggtggcgt ggccgcgggc atcccagcgc agcacgtggt
     5881 gatcggccga caagacatcc acgaagggat tccagtcccg gtgggacgac gtccaaccgt
     5941 gcaggaggat gaccggcggc ccctcgcccg acacctggac gtggagacat tcgccatcgt
     6001 cggcgatgaa gtgctgcatg acgctcaccc ggagaaggac acaggggatc gagtatagcc
     6061 gcccccggcc gctgcctcag acccggaagc gcgccaccgt ctcctgcagc tcactggcca
     6121 gccgctccag atcgcgggcg gcgtcggctg tggattccac tgccccattg ctggcccggg
     6181 ccatctctgc catggcatcg atgctttccg ccacacgacg gctcgccgcg cgctgacgct
     6241 cggtggcgcc ggcgatttcg tcgagaccgc ggcctacggc ttcgaccgac tcgttggccg
     6301 aaacgatcac ggctgatacc tcgccgacgg ccacccggct gctttccaga tgctcgagcc
     6361 cttcgtggat ggcttgacgc acggccaccg actgctgaga caggcgcgag gtgaccgcgt
     6421 cgatctcacc cgccgaccgg gccgacttct cggccagctt gcgtacctcg tcggccacca
     6481 ccgcgaaccc ccgcccctgt tcgccggccc gcgcggcctc gatcgcggcg ttcagggcga
     6541 gcagattggt ctgctccgcg atctcccgca cttcctgagt catggtggtg atggcgctgg
     6601 tggattcgac gaactgaccg accgagtcgg ccatctggcg taccgaggct tccgcctgtc
     6661 cgacttcacc caccagcatg tccagacttc gactgccgtc ctgggaacgg cgcagactct
     6721 cgcgggatcg ctggtgcacg ccttcggtgc tgaccgccac atgggcgata tccgaggcca
     6781 tggtctccac ctcgctggcc gcgctggcgg actgctcgca ctgggcgcgc gcaccctccg
     6841 ccacgcgact cgcgctcatc gtcagctgat gggacgacgc ggtcacccgc gacgcggaat
     6901 cggacacccg ccgcaccaga ctggcgaccg tgccgagcat ggcattgaag gcgtcggcgg
     6961 cctgcccgat ctcgtcctga ccgcgtaccg gcaggcggcg cgtcagatcg ccctccccct
     7021 tggcgatctc atgcagactg cggttcatgt ccgccagggg gcgggccacg aagctgcgga
     7081 tgaagtaggt gacgaacagg atcagcggaa gggacagcac cagcgcgctg atgatgcttt
     7141 tccagcggaa ggactccacg gcctcgttca ccttgtccag cgataccttc atgctgacca
     7201 gtcccagggg cgtacccgcc gggacaaggt ggcagccaat gcagttcttg ccaagatagt
     7261 tgtcggacgc aatggcaggg cggatcaccc gtagatattc accaagacgc ggatcggtct
     7321 gaattcggct gaccgcctgc cccgtcgcca gcaccgcctg ctcatcggca tccaggggcg
     7381 gctgggggcg cgtgccgggc ccgaactggc ggctcacggc gtcgccccgc aggacctgca
     7441 ggtcatggat gatcgacagc tgcttgatct gatcaagaaa gacttcgcgc tgcccgacgg
     7501 tgccggtgat catcatgccg gtcagacctg ccatggtcat ctcgtggatg ctgcgcgcga
     7561 aatcctcggc ctgggcgacg gcggtctccc ggttggtgcg tgtctcccac acgatcatgc
     7621 cgccccaggc gatcaccagc atcagccaga tggccgccgt caatctcagc cagatgggcg
     7681 tatccgcaag ccttcgcatc tccatatccc ctcaaggacg ggaactcgct gccccgtttg
     7741 gtgagtcgct gtatcgggac tggactacgg cgcacgcggg ggatgcttta gctttttggc
     7801 ggaattttcg agattattga ctgaattgat gaaaaaacag gtttcgctta cttcttcgca
     7861 tcacccggcg gacgggcatt gatgcgctgc gtcgcggcgt tccagtcgcc cgcggcaata
     7921 cggggccaga cggccagcgc ccggtcgagg gcatcgtcga tgagggccgc ctcctcgcgc
     7981 cgcggcggct tgaggacaaa gttcaccact tcgttgcgat cgcctggatg tccgatgccg
     8041 atgcgcaggc gccagtagtc ctgggtgccc aggtgggcgg tgatgtcctt gaggccgtta
     8101 tggccgccga ggccgccgcc gaacttgagg cgcaggtgcg ccggcgggat gtccagctcg
     8161 tcgtggacca ccaggatctc ggccggcggt atccggtaga agcgtgccag cgccgccacg
     8221 gcctgaccgg agcggttcat gaaggtctgg ggcatgagca gccatacgcc ggcgtcgcgg
     8281 gcgtgggcgg ccaggccatg gaagcgggct tccttcgaaa aggaggcgcc cagttcccgc
     8341 gccagccgct cacaaaacca aaacccggcg ttatgccggg tttcggaata ttcggcgccg
     8401 ggattcccca ggccgacgac aagccggggc gcagtctggc tcatcgcagg acggccccgg
     8461 tcatccggtt gcggatcagg ccgccggggt ttcggcagcg gcttcgtcct cgccaccgcc
     8521 acggaccgcc aggatggtgg ccacgaccgg gtcttcaccg tgggtcaggg ccttcacacc
     8581 ggccggcagg ggcaggtgcg aaacgtggat ggacgcgcca cccttgagat ccttcaggtc
     8641 cacctcgacg aactcgggca gatcgccggg caggcattcc agatccagtt cggtcatcac
     8701 gtgagacacg atgccgcctt cgagcttgac gcccggggcg atgtcggcgt tcacgaagtg
     8761 caggggcacc ttcacgtgga tcttgtgagt ggaatcgacg cgctggaagt ccaggtgcag
     8821 cacttgctgc ttgtacgggt gccactgggt gtcccgcagg atcacggcct gggcagcccc
     8881 gtccaccagg gcggtgagca cggaggagtg gaaggcttcg ttgcgcagca ggtggaagat
     8941 ctcgttgtga tccagttcca cttgcagcgg ggcgacttcg ccaccgtaga tgatggcggg
     9001 cacgcggccg gcgcgacgca ggcggcggct cgcactcgaa ccctgcgctt cacgcttgga
     9061 ggcattgaat tgaatggtca tgcttgttgc tccaggttga tgggcccgtc cgcgaccaga
     9121 cgggctgggg tgatggccgg caaacggggt ccgccagccg aaaaaattca ctcggtgaac
     9181 agggaactca ccgattcctc gttgctgatg cgcacgatgg tgtcggccag cagggaggcg
     9241 atggagaccg tgcggatgcg cgaacaggcg cgcgcttcgt cggtcagcgg aatggtatcg
     9301 gtgaccacca gttcgtccag gtcggaatcc atgatccgcg acacggcggg gccggacagc
     9361 acggcgtggg tgcagtaggc catcacccgc ttggcaccgt tctccttgag ggcctgagcc
     9421 gccttgcaca gcgtgccggc ggtgtcgacg atgtcgtcca tgatcacgca ggtacgcccg
     9481 tcgacctcac cgatgatgtt catcacctcg gacacattgg ccttgggacg acgcttgtcg
     9541 atgatcgcca gatcgcattc caggcgcttg gcaaaggccc gcgcccgcac cacgccaccc
     9601 acgtccggcg acacgaccaa caagtcgtcg tacttctgct tctcgagatc gtcgaggagg
     9661 accggtgcgg cgtagatgtt gtcgacggga atgtcgaaga agccctggat ctggtcggca
     9721 tggagatcca cggtgaggac acgctgcacg cccacggcct gaagcatgtt ggccaccacc
     9781 ttggcggtga tgggcacccg cgccgagcgc ggccggcggt cctgccgggc ataaccaaaa
     9841 taggggattg cggcggtgat gcggccggcg gaggcgcgct tcagcgcgtc caccatgatc
     9901 atcagctcca tgatattgtc attcgtggga gcgccggtgg gttgcaggac gaagacgtcc
     9961 ttgccacgca cattctccag cagttccaca ttgacttcac cgtcggagaa acggcccacc
    10021 gtggccgacc ccagagaccg tcccaggcgc ctgaccacat ccgcggccag cttggggttg
    10081 gcgttgccgg tgaataccat caggctgccc gaagccatga tcacctctcg tgttgctgca
    10141 tcgttgaacc gctaaaacac aaacgggcag gctttcggcc tgcccgctgt cgtttggctg
    10201 gggaggaagg attcgaaccc tcgaatgccg gaatcaaaat ccggtgcct
//
LOCUS       DAOQMM010000124         9763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3310_length_9763_cov_42.889576, whole genome shotgun sequence.
ACCESSION   DAOQMM010000124 DAOQMM010000000
VERSION     DAOQMM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_9763_cov_42.889576"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..2244)
                     /locus_tag="PLH85_15340"
     CDS             complement(244..2244)
                     /locus_tag="PLH85_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNB65992.1"
                     /translation="MPFSERGPLVPRISDDDIVASAWESFVRNAAVSGPGLRSVVLDS
                     WQRSRAGAVDPGRHSAPGADMERVRRLLWQNRELCEVARPVLEGLREVLRECGSLIML
                     SDPAGTILDLNGGTRARSVGEEINLATGGCWSEALIGTNAIGTAIATRAPVQIYANEH
                     FCVDVKRWTCAAAPILDRSGRVRGVVDVSGVKETFHGHTLGLVMAAARQIEADFAHRD
                     EVLNSRLLVAAITDFSRYSSDCLLLVDHRGRILRVAGDLGAVRTRLHLPDRLDVGSVL
                     AALELPQDGESRALHLPGWVQPEWLRPIKDGPLRLGTLIVIPTVAGRTSPARASSATS
                     PADLGTDAFADIIGNSEVLQIAKNRARRIAPLDLPVLLLGETGAGKELFARALHRAGN
                     RPEGPFVAVNCGALTRELLASELFGYAEGAFTGARRGGHPGKFEQAHGGTLFLDEIGE
                     MPIDMQPYLLRAIQDGVIVRVGDTRERRVSVRLVAATNRDLPDAIAAGRFREDLYHRL
                     GVVTLNLPALRDRPGDIDSIVDHLNRRLADKYGGTPKVLTPAVRAALQAYRWPGNIRE
                     LQNVIEVMFALCEGDTIDVPLLPQTLTRSEATRPLAARPAAVTAPVPAGRLDDVQRQA
                     IQAAVANARGNLSLAARTLGISRSTLYVKLASMRPADETSVR"
     gene            2588..4732
                     /locus_tag="PLH85_15345"
     CDS             2588..4732
                     /locus_tag="PLH85_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNB65993.1"
                     /translation="MQQRSTVQVMGIDAGGTMTDTFFVRDDGRFVVGKAQSNPGDESL
                     AIYNSSVDALAHWNRTVDDVYPELVTCVYSGTAMLNRILMRKGLDVGLICNKGFEQIH
                     SMGRALQSYLGYALEDRIHLNTHRYDEPLVPVSRTRGVTERTDVQGRVVIPLREDEVR
                     AATRELVEAGAQAIVICLLQSHKNEASEQRARDLVREELARLGVELPVFASVDYYPSR
                     KESHRMNTTILEAYGAEPSRQTLRKVSDRFKKHGARFDLRVMATHGGTISWKAKELAR
                     TIVSGPIGGVIGSRLLGEALGDENIACSDIGGTSFDVALITKGNFAIKSDPDMARLVL
                     SLPLVAMDSVGAGAGSFVRLDPYSKSIKLGPDSAGYRVGTCWPESGLDTVSVSDCHVV
                     LGYLNPNNFLGGAIKLDVERARQHIKAQIADPLGLSVEDAAAGVIELLDLTLSEYLRA
                     NISAKGYNPADFTCFSYGGAGPVHTYGYTEGVGFKDVVVPAWAAGFSAFGCACADFEY
                     RYDKSVDLGVAQHAADTVKSAACRTLQDAWSELAVKVIDEFVLNGYKAEDVILIPGYK
                     MQYMGQLNDLEIVSPVSSASSAADWDRIVDAFETTYGRVYASSARSPELGFSITGAIL
                     RGMVVTQKPVLPEDADGGPTPPKEAYLGTRPFYRHKKWVDAALWKMEALKAGNHIVGP
                     AIIESDATTFVVPDGFETTIDKHRLFHLKEVL"
     gene            4781..7102
                     /locus_tag="PLH85_15350"
     CDS             4781..7102
                     /locus_tag="PLH85_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNB65994.1"
                     /translation="MNMLSKKEVGFADLLKNGQTLKEFRDGIIERTQASGHYNGLERL
                     EFRDRDPIGYEKLFSKLRGGLVHARETAKKIAASPIVEQEGELCFTLYNAVGDCVLTS
                     TGIIIHVGTMGAAIKYMIENNWEANPGIRPGDMFTNNDCSIGNVHPCDIATIVPIFWE
                     GRLIGWVGGVTHVIDTGSVTPGSMSTGQTQRFGDGYMITCRKTGINDEPLRDWLHESQ
                     RSVRTPKYWILDERTRIAGCHMIRDLVEEVIRADGIEAYEKFAYEVIEEGRRGLMSRI
                     KAMTLPGKYRKVSFVDVPYKHEDVQVSNAFAKLDSIMHSPCEMTIRPDGKWRLDFEGA
                     SRWGWHTFNAHQVAFTSGIWVMMCQTLVPTQRINDGAYFATEFRLPKGTWCNPDDRRT
                     GHAYAWHFLVSGWAALWRGLSQSYFSRGYLEEVNAGNANTSNWLQGGGINQDGEIHAV
                     NSFEASSCGTGACAIKDGLNHAAAIWNPEGDMGDIEIWEMAEPLLYLGRNVKANSGGY
                     GKYRGGCGFETLRMVWNAQDWTMFFMGNGFMNSDWGMMGGYPSATGYRFEAHKTGLEK
                     RIEIGDSLPLGGDLDPSDPDYERHLDATATVKRDKQCITTEDCYANHDLYLNYLRGGP
                     GFGDPIDREPKAIEADLNQKFLLPEYARKVYGAVFTEDGGVFTVDVAKTAERRTEIRK
                     ERLARALPTREWMKAERERILNKHASTQVKHMFATSFALSEKFTQQFKDFWHLPADWQ
                     LLEDELDVPTYGSKHRMDLSLLPDVTTVVQVEE"
     gene            7126..7635
                     /locus_tag="PLH85_15355"
     CDS             7126..7635
                     /locus_tag="PLH85_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HNB65995.1"
                     /translation="MSTYTNEQVTHLVEGTLDWETTFRMLSMPKDHGRFEQYLAALQA
                     KVDFADRIILPLGPHLYIVQQASTRKWVTQCDCGHVFGDYRENWKLNASVYVRRTEEA
                     MTEVYPRLMAPDTEWQVYREYYCPQCGAMHDVEAPTPWYPVIHDFEPDIEAFYKEWVN
                     LPLPERAPD"
     gene            7722..9119
                     /locus_tag="PLH85_15360"
     CDS             7722..9119
                     /locus_tag="PLH85_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HNB65996.1"
                     /translation="MCKVYPNAAAALDGLLFDGMTVAAGGFGLCGIPENLIAALLAAG
                     TRELTIVGNNAGVDDFGMGLLLKSRQVKKVVASYVGENKEFERQVLAGELELELVPQG
                     TLAERLRAGGAGIPGFYTRTAFGTLLADGKETRRFGSKDYVLEPAIRADVSIVKAWKG
                     DRAGNLVFRKTARNFNPMIATCGSLCIAEVETLVDVGELDPDEIHTPGIYVNRIIAGA
                     DYQKRIEFRTVAGSASPARHDPVRDRMARRAAQELRSGYYVNLGIGIPTLVANHIPPG
                     VQVTLQSENGLLGIGPSPEATAVDADLINAGKQTITTLPGSSFFSSADSFAMIRGGHI
                     DLSILGGLEVSEQGDLANWMVPGRMVKGPGGAMDLVSGVGRVVVLMEHCAKDGSPKIL
                     RECSLPLTGKGVVDTLITDLAVFKVKEGGGLLLTELQPGVSVEDVRERTGCGFSVAPQ
                     VASGVPATIRTGART"
     gene            9177..9587
                     /gene="pcaC"
                     /locus_tag="PLH85_15365"
     CDS             9177..9587
                     /gene="pcaC"
                     /locus_tag="PLH85_15365"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HNB65997.1"
                     /translation="MSERFERGLAMRRQVMGDAHVERAFASATGFSRPLQEFMTEHAW
                     GTVWCRDGIDLKTRSLITVAMLAALGRGQELAGHVRGALHNGASADEVREVLLHAAVY
                     VGVPLAAEAVRIAEAVVRERPDASPPLDSPSPSR"
BASE COUNT         1733 a         3274 c         3220 g         1536 t
ORIGIN      
        1 atcgcggccc aggcggtcgc gctgctggcg cgcgtggaga cctgaggcgc ggacggcgca
       61 accctgacga cgcgccgggg agcgtcggca tcgcgcgctg tggcgcggcg ccgcgcgccc
      121 atcgcgggcg gtgattccgg gagccgatga tggaccgggc cgcagatcgg ggcgtgaccg
      181 tgccttcagg cccgcctccg gtcctcagct aggggggggg ccgtgtggtc cgtcgcgctg
      241 ccgtcagcgc acagaggtct cgtccgcggg gcgcatgctt gccagcttga catacagggt
      301 gctgcgggag attcccaggg tccgcgcggc cagggacagg ttgccccgcg cgttggcgac
      361 cgcagcctgg atcgcctggc gttgcacgtc gtcgaggcgg ccggcgggca cgggggcggt
      421 tacggcggcc ggacgggcgg ccaatgggcg ggttgcctcg ctgcgggtga gtgtctgggg
      481 cagcagcggg acatcgatcg tgtcgccctc acagagcgcg aacatcacct cgatcacgtt
      541 ctgcaactcg cggatgttgc cgggccagcg gtaggcctgc aacgctgcac gcaccgcggg
      601 cgtgagcact ttgggcgtac ccccgtattt gtcggcaagg cgccggttga ggtggtcgac
      661 gatgctgtcg atgtcgcccg ggcgatcgcg cagcgcgggt agattcaggg tgacgacgcc
      721 caggcggtga tacagatcct cccggaaacg ccccgcggcg atggcgtcgg gcagatcccg
      781 gttggtggcg gccaccagcc gcaccgagac gcgccgctca cgcgtgtcac cgacccgcac
      841 gatcaccccg tcctggatcg cccgcagcag gtacggctgc atgtcgatcg gcatttcacc
      901 gatttcatcg agaaacagcg tcccgccgtg ggcctgttcg aacttgcccg gatgcccccc
      961 gcgacgggcc ccggtgaagg cgccttcggc gtagccgaac agctcgctgg ccagcagctc
     1021 ccgcgtgagg gcgccacagt tcactgccac gaacgggccc tccgggcgat ttcccgccct
     1081 gtgcagggcg cgggcaaaca gttccttgcc ggcaccggtc tcgcccagca gcagcaccgg
     1141 caggtccagc ggggcaatac gccgggcccg gttcttggcg atctgcagaa cctcgctgtt
     1201 gccgatgatg tcggcgaagg cgtccgtgcc gaggtcggcg ggtgatgtcg cgctgctggc
     1261 gcgtgcgggc gatgtccgcc cggcgacggt cgggatcacg atgagggttc ccaggcgcag
     1321 cggcccgtcc ttgatcgggc ggagccattc gggctgcacc cagccaggca ggtggagggc
     1381 gcgtgactcg ccgtcctggg gcagttcgag ggcggcgagc acgctgccca cgtccaggcg
     1441 gtccggcagg tggaggcggg tacggactgc gcccagatcc cctgcaacgc gcaggatgcg
     1501 tccccggtga tccaccagca gcaggcagtc actgctgtaa cgggaaaaat cggttatggc
     1561 ggcgaccagc aggcggctgt tgagcacttc gtcccggtgg gcgaagtcgg cttcgatctg
     1621 ccgcgcggca gccatgacca gccccagggt gtggccgtga aaggtctctt tcacgcctga
     1681 cacatccacc acaccgcgca cccggccgct gcggtcgaga atcggcgcgg cggcacaggt
     1741 ccagcgcttc acgtccacgc agaaatgttc gttggcgtag atctggaccg gtgcccgcgt
     1801 ggcgatggcc gttccgatgg cgttggtgcc gatcagcgcc tcgctccagc agccgccggt
     1861 ggcgaggttg atctcctcgc ccacgctgcg ggcacgggtg ccaccgttca ggtcgaggat
     1921 cgtgccagcc ggatcgctca gcatgatgag gctgccacat tcgcgcagca cctcgcgcag
     1981 gccttccagc accgggcggg ccacttcaca cagctcacgg ttctgccaca gcagacggcg
     2041 cacccgttcc atgtcggcgc ccggtgccga atggcggccc ggatcgacgg ccccggcccg
     2101 gctgcgttgc cacgagtcga gcaccacgct gcgcagtccc ggtcccgata cggcggcgtt
     2161 ccgcacgaag gattcccacg ccgacgcgac gatgtcgtca tcgctgatgc gtggaacgag
     2221 ggggccgcgc tcggaaaagg gcatggggcg gggaacaggc gtccgggaaa aggtcgcgac
     2281 caccgtagcg tcaagcgcgg acaaagtcaa taaagtcctt aaagatcaat gatttgtgcg
     2341 ttgcgcaaaa gtgtccggat tccggacagg tgagggccgg cgtcaggaat ccggacatcg
     2401 catcggacag accggcgacg ccggaggacg gtcggacgaa gcgcgccgcg tcccctgctg
     2461 atttgttcat ctgattgaat cataaggaca gtttctgcgt gggccgcatg cggtccggtg
     2521 tctggcccgg cctttgcacc ggggtgtccg tggggcaagc ccactcacac caacgacagg
     2581 aggcaacatg caacaacgat ccaccgtcca ggtcatgggc atcgatgccg gcggcacgat
     2641 gaccgatacc ttctttgtcc gcgacgacgg ccgcttcgtg gtcggcaagg cccagagcaa
     2701 tccgggcgat gaatccctcg ccatctacaa ctcgtcggta gacgcgctgg cgcactggaa
     2761 ccgtacggtc gacgacgtct acccggaact ggtgacctgt gtgtactcgg gtactgcgat
     2821 gctcaaccgc atcctgatgc gcaaggggct cgatgtcggc ctgatctgca acaagggctt
     2881 cgaacagatc cactcgatgg gtcgcgccct gcagagctac ctgggctacg ccctggaaga
     2941 ccgcatccac ctcaacacgc accgttacga cgagccgttg gtcccggtct cgcgcacccg
     3001 cggcgtgacc gagcgtaccg acgtgcaggg gcgcgtggtc attcccctgc gcgaggacga
     3061 ggtccgcgca gccacccggg agctggtcga ggctggcgcc caggccatcg tcatctgcct
     3121 gctccagtcc cacaagaacg aggccagcga gcagcgtgcc cgggatctcg tgcgggaaga
     3181 gctggcccgg ctgggggtgg agctcccggt gttcgcgtcg gtggattact acccctcgcg
     3241 caaggaaagc caccggatga acaccaccat cctggaggcc tacggcgcgg agccgtcgcg
     3301 ccagaccctc aggaaggtga gcgaccgctt caagaagcac ggtgcccggt tcgatctgcg
     3361 ggtcatggcc acccacggcg gcacgatcag ctggaaagcc aaggagctcg cccgcaccat
     3421 cgtttccggt cccatcggcg gcgtgatcgg ctcccgcctg ctgggcgaag cgctgggcga
     3481 cgagaacatt gcctgctcgg acatcggcgg aaccagcttc gacgtggcgc tgatcaccaa
     3541 gggcaacttc gccatcaaga gcgatcccga catggcccgc ctggtcctgt cgctgcccct
     3601 cgtggccatg gattccgtgg gcgccggcgc gggcagcttc gtgcgcctcg atccctacag
     3661 caaatcgatc aagctggggc cggattcggc gggctatcgg gtgggcacgt gctggccgga
     3721 aagcggcctc gacacggtgt cggtctccga ctgccacgtg gtgctgggct acctgaaccc
     3781 caacaacttc ctcggcggcg ccatcaagct ggacgtggag cgcgcccgtc agcacatcaa
     3841 ggcacagatt gccgatccgc tgggcctctc ggttgaggac gcagccgccg gggtcatcga
     3901 actgctcgac ctgacgctct cggaatatct gcgcgccaac atcagcgcca aggggtacaa
     3961 ccctgccgac ttcacctgct tctcctacgg cggcgcgggg ccggtccata cctatggcta
     4021 caccgagggt gtgggcttca aggatgtggt ggtgccggcc tgggcggcag gtttctccgc
     4081 gttcgggtgc gcctgtgccg attttgaata ccgctacgac aagtcggtgg atctgggcgt
     4141 cgcccagcac gcggccgaca ccgtgaagtc cgccgcctgc cgcaccctgc aggacgcctg
     4201 gtccgagctg gcggtcaagg tcatcgacga gttcgtcctc aacggctaca aggccgagga
     4261 cgtgatcctg atccccggct acaagatgca gtacatgggc cagctgaatg acctggagat
     4321 cgtctctccg gtgtcctcgg catcctcggc ggcggactgg gaccgcatcg tcgacgcctt
     4381 cgagaccacc tatggccggg tctatgccag ctcggcccgc tcgcccgagc tgggcttctc
     4441 gatcaccggc gcgatcctgc gcggcatggt ggtgacccag aagccggtgc tgccggagga
     4501 cgcggacggc ggtccgacgc cgccgaagga ggcttacctg ggcacccgcc cgttctaccg
     4561 ccacaagaag tgggtggacg ccgccctgtg gaagatggaa gcgctcaagg ccggcaacca
     4621 catcgtcggg cccgccatca tcgaatcgga cgccaccacc tttgtcgtgc cggacggctt
     4681 cgagaccacc atcgacaagc accgcctgtt ccacctgaag gaagtcctct gacaccgcgc
     4741 agctgcccgg gtggaccaca acgatctagg agacaaggta atgaatatgt tgagcaagaa
     4801 ggaagtcggc ttcgccgatc tgctgaagaa cggccagacc ctgaaggaat tccgcgacgg
     4861 gatcatcgag cggacccagg ccagcgggca ctacaacggt ctcgagcgcc tggaattccg
     4921 ggaccgcgac ccgatcggct acgagaagct gttctcgaaa ctgcgcggcg gcctggtgca
     4981 tgcccgcgaa acggccaaga agatcgccgc cagccccatc gtcgagcagg aaggggagct
     5041 gtgcttcacg ttgtacaacg cggtcggcga ctgtgtgctc acctccaccg gcatcatcat
     5101 ccacgtgggg acgatggggg cggcgatcaa atacatgatc gagaacaact gggaggccaa
     5161 ccccggcatc cgccccggtg acatgttcac caacaacgac tgctccatcg gcaacgtgca
     5221 tccctgcgac atcgccacca tcgtgccgat cttctgggaa ggccggctga tcggctgggt
     5281 gggcggcgtc acccacgtga tcgacaccgg ctcggtcacg cccggctcca tgtccaccgg
     5341 tcagacccag cgcttcggtg acggttacat gatcacctgc cgcaagaccg ggatcaacga
     5401 cgaacccctg cgtgactggc tgcatgaatc ccagcgctcg gtgcgcacgc ccaagtactg
     5461 gatcctcgac gagcgcacgc gcatcgccgg ctgtcacatg atccgtgacc tggtggaaga
     5521 ggtgatccgc gccgacggca tcgaggccta cgagaagttc gcctacgaag tcatcgaaga
     5581 agggcgccgt ggcctgatga gccgcatcaa ggccatgacc ctgccgggca agtatcgcaa
     5641 ggtctccttc gtggatgtgc catacaagca cgaggacgtg caggtctcca acgccttcgc
     5701 caagctcgat tccatcatgc actcgccctg cgagatgacg atccggccgg acggcaagtg
     5761 gcggctggac ttcgaggggg cgagccgctg gggctggcac accttcaacg cccaccaggt
     5821 ggcatttacc tcgggcatct gggtgatgat gtgccagacc ctggtgccga cccagcgcat
     5881 caacgacggc gcctacttcg ccaccgagtt ccgcctgccc aagggcacct ggtgcaatcc
     5941 cgatgaccgc cgcacgggcc acgcctacgc ctggcacttc ctcgtctccg gctgggcggc
     6001 attgtggcgg ggcctgtccc agtcgtactt cagccggggc tacctggagg aggtcaacgc
     6061 cggcaacgcc aatacctcca actggctgca ggggggcggc atcaaccagg acggcgagat
     6121 ccacgcagtc aacagcttcg aggcgagctc ctgcggtacc ggcgcctgcg ccatcaagga
     6181 cgggctcaac catgcggcgg cgatctggaa cccggaaggt gacatgggcg acatcgagat
     6241 ctgggaaatg gccgaaccgc ttctctacct gggacgcaac gtcaaggcca actccggcgg
     6301 ctacggcaag taccggggcg gctgcggctt cgagaccctg cgcatggtct ggaatgccca
     6361 ggactggacc atgttcttca tgggcaacgg cttcatgaac agcgactggg gcatgatggg
     6421 cggctatccg tcggccaccg gctaccgctt cgaggcccac aagaccgggc tcgagaagcg
     6481 catcgagatc ggcgacagcc tgcccctggg gggcgatctc gatccgtccg atccggacta
     6541 cgagcgccac ctcgacgcca ccgccacggt caagcgcgac aagcagtgca tcaccaccga
     6601 agactgctac gccaaccacg acctgtacct caactacctg cgcggcggcc ccggcttcgg
     6661 tgatcccatc gaccgtgagc cgaaggccat cgaggcagac ctcaaccaga agttcctgct
     6721 cccggaatac gcccggaagg tctatggcgc ggtgttcacc gaagacgggg gcgtgttcac
     6781 cgtcgatgtc gccaagaccg ccgaacggcg caccgagatc cgcaaggaac gcctggcccg
     6841 cgccctgccg acccgcgaat ggatgaaggc ggaacgcgag cgcatcctca acaagcacgc
     6901 ctcgacccag gtcaagcaca tgtttgccac cagcttcgcc ctgtccgaga agttcacgca
     6961 gcagttcaag gacttctggc atctgccggc cgactggcag ctgctcgagg acgagctgga
     7021 tgtcccgacc tatggttcca agcaccgcat ggacctgtcc ctgctgccgg acgtcaccac
     7081 cgtcgtccag gtcgaagaat aaccaccgaa tcaaggagtc aatcaatgtc tacctacacc
     7141 aacgagcagg tcacccacct tgtcgagggc acgctggact gggaaaccac cttccgcatg
     7201 ctgtccatgc ccaaggacca cggtcgcttc gagcaatacc tggcagccct gcaggccaag
     7261 gtcgatttcg cggaccgcat catcctgccg ctcggcccgc acctctacat cgtgcagcag
     7321 gccagcacca ggaaatgggt gacccagtgc gattgcggcc atgtctttgg cgactaccgc
     7381 gagaactgga agctcaacgc ctcggtgtac gtgcgccgca ccgaagaggc catgaccgag
     7441 gtctatccca ggctgatggc ccccgacacc gagtggcagg tgtatcgcga gtactactgc
     7501 ccccaatgcg gcgccatgca cgacgtcgag gcgccgaccc cgtggtatcc ggtgatccat
     7561 gacttcgagc cggacatcga agccttctac aaggaatggg tgaatctgcc gctgccggag
     7621 cgggcgccgg actgagcctg cgattgaacg gcccccgggg ccgtcaagcg gggacgggcg
     7681 ggggcctgtc cccgcccgac aagaacagcg gagacacacc catgtgcaag gtttatccca
     7741 acgctgccgc agcgctggac ggactgctct tcgacggcat gaccgttgcg gccggcggct
     7801 tcggcctctg cggcatcccc gagaacctca ttgccgcgct gctggcggcg ggcacccgcg
     7861 agctgaccat cgtcggcaac aacgcaggcg tggacgattt cggcatggga ctgctcctca
     7921 agtcccgcca ggtgaagaag gtcgtcgcct cctacgtggg cgagaacaag gagttcgagc
     7981 gtcaggtgct ggcgggcgaa ctggagctgg aactggtgcc ccagggcacc ctggccgaac
     8041 gcctgcgggc cggtggtgca ggcattcccg gcttctacac gcgtaccgcc tttggcaccc
     8101 tgctcgccga cggcaaggag acccgccgtt ttggcagcaa ggactacgtg ctggagccgg
     8161 ccatccgcgc cgatgtctcc atcgtcaagg cgtggaaggg ggaccgggcg ggcaacctgg
     8221 tcttccgcaa gaccgcccgc aacttcaacc cgatgatcgc cacctgcggc agtctgtgca
     8281 tcgccgaggt cgagacgctc gtggatgtcg gcgaactgga cccggacgag atccacacgc
     8341 cgggcatcta cgtgaaccgg atcatcgcgg gggccgatta ccagaagcgg atcgaattcc
     8401 ggaccgtggc cggcagcgcc tcgcccgccc gccacgaccc cgtgcgcgac cgcatggccc
     8461 gccgcgccgc acaggagctg cgcagcggct actacgtgaa cctggggatc ggcattccga
     8521 ccctggtcgc caaccatatc ccgccaggtg tccaggtcac cctgcaatcg gagaacggcc
     8581 tgctgggcat cggcccgtcg cccgaggcca cggcggtcga tgcagacctc atcaacgccg
     8641 gcaagcagac catcacgacc ctgcccggca gcagcttctt ttccagcgcg gattccttcg
     8701 ccatgatccg cggcggtcac atcgatctgt ccatcctggg cggcctggag gtgtcggagc
     8761 agggcgatct ggccaactgg atggtgcccg gccggatggt caagggcccc ggcggcgcga
     8821 tggacctggt ctccggtgtc gggcgggtcg tcgtcctgat ggaacactgc gccaaggacg
     8881 gttcgcccaa gattctccgc gaatgctcgc tgcccctgac cggcaagggc gtggtggaca
     8941 ccctcatcac cgatctggcc gtgttcaagg tgaaagaggg cggcggcctg ctgctgaccg
     9001 aactgcaacc cggcgtcagc gtggaggacg tccgcgaacg aaccggctgc ggcttttccg
     9061 tggcaccgca ggtcgcaagc ggcgtgccgg cgacgatccg cactggagca cggacatgat
     9121 gggcgccgcc agcagcgtgc gtcccgtccg ggacggcacc gtgagggaga cacagcatgt
     9181 ctgaacgttt cgagcgcggt ctggcgatgc gtcgccaggt gatgggcgac gcccatgtcg
     9241 agcgggcgtt tgcatcggcc accggcttct cccgtcccct gcaggagttc atgaccgagc
     9301 acgcgtgggg cacggtgtgg tgtcgcgacg gcatcgacct caagacccgc agcctgatca
     9361 ccgtcgcgat gctcgccgcc ctcgggcgcg ggcaggagct ggcagggcat gtgcggggcg
     9421 ccctccacaa cggcgcgagc gccgacgagg tccgcgaagt cctgctgcat gccgccgtct
     9481 atgtgggtgt tcccctggcg gccgaggcgg tccgcatcgc cgaagccgtc gtccgggagc
     9541 ggcccgacgc atcgcctccc ctcgattctc cgtcgccatc gcgttgaacg ctcgaagacg
     9601 cgaggcagac cgacggtgaa tctttggccc gctccggcgg gccgcttttt tgaagcgacg
     9661 catgggctgc acgggccggc ctacggcggc ccccacacgc tgcacgtacc aaaaaaaagc
     9721 cggcccggag gccggcaaat tcctaggtgc cagagaatga aat
//
LOCUS       DAOQMM010000125         9701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3341_length_9701_cov_48.849990, whole genome shotgun sequence.
ACCESSION   DAOQMM010000125 DAOQMM010000000
VERSION     DAOQMM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3341_length_9701_cov_48.849990"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="glnS"
                     /locus_tag="PLH85_15370"
     CDS             <1..1182
                     /gene="glnS"
                     /locus_tag="PLH85_15370"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNB65998.1"
                     /translation="IDMASPNINLRDPAIYRIRHATHHRTGDRWCVYPMYTYAHPIED
                     ALEDITHSICTLEFEDQRPFYDWLLDALADGGFFPRPLPQQIEFARLNLTYVVLSKRK
                     LIQLVDEGHVAGWDDPRLPTLVGARRRGFTPEGFRQFAERIGVSKADSWIDMSVLEDC
                     MREHLNEAAERRVAVLDPVKLIITNYPEGQVERCHAPNHPLKPELGKREMPFGRELWI
                     EREDFMETPVKGYHRLYPGNTARLRYGYVVRCTGCEKDADGKVTAVLCEYFPDSKSGT
                     PGADTYKVKGNLHWVAVAHAYAAEVRLYDRLFAHPYPGTRREGDPEDLQRDFLDDINP
                     DSLRVIRAQLEPALRAARPEARFQFERHGYFVADRIDTQEGAPVFNRTVTLRDAWKHG
                     A"
     gene            1370..3814
                     /locus_tag="PLH85_15375"
     CDS             1370..3814
                     /locus_tag="PLH85_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB65999.1"
                     /translation="MLSNLSIRVRLLLASTVVQVVMLTLLLTNSARLMNEATGASLET
                     LISQNAAILNIVTASFVPQGRYAELQDALGELLNDSSEGLVYVRIVDASGHTQVRAGM
                     PDLATLPPPDNPATLTLPAGESRNVINIRRPLLMDRNQVGFLQFGVSVSALTVAKQQI
                     LSQGLFIATAEVLLTLLLLGTIGYLMTRNLGRLLRGSQAIAAGDLSHRIPEKGQDELA
                     QLCKHFNRMALALQSRIHELEATARQLAQSEERYALATRAANDGLWDWDIVTGKTYFA
                     PRFAEILGITPDAFVGDRDTLFKHIHPEDREAFRLRLVAHLKRETAQLNVEHRIRHAD
                     GSYRWTLTCGMAVYDPDSGRAIRMAGSISDIHLRRRAEDQLVHDALHDGLTGLANRAL
                     FVEHLQNTLRHADRVTGSLFAVLHVNIERFHVVNDSLGYSAGDDLLRLVGQRIQALAR
                     PGDVVGRVGGDQFALLLNDIADASEAIRFSENLQTRLAESAELNGHSLYPKTRIGVAL
                     GSGGFHNAEDLMRDADNAMHRSRSSSDSTITVFQSTMHQQALRDLQLESQLRIALQEG
                     QFVAHYQPIVHLADGRTAGFEALVRWPEQATRRIGPDVFVPLAESLDLIHELGMHMLD
                     QSCAQLKAWRTLGLADGDLSISVNLSARQLMQVDLADQVVECIERHELPPSALKLEIT
                     EGTLVTRKELATSQLGKLRAKGLKILIDDFGTGYSSLSYLHTIPCDTLKLDGSFIREI
                     ERDTRLRAIVGATIRLSHELGISVVAECIETPAQAQLLRDLGCDYGQGYLFSSAQPGD
                     KAQQWLLNNLPVGAGA"
     gene            3811..4998
                     /locus_tag="PLH85_15380"
     CDS             3811..4998
                     /locus_tag="PLH85_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66000.1"
                     /translation="MKRIAALLILGASLPVAADEPSRFSFSGFGTVGAVRTSNDVLGF
                     RSSVVQDTGPGRAFDTSVDSVIGVQGSAHLTGKSDITLQVVSHKGPDDAFTPQVTWSF
                     LRTQLLPELSVRAGRMRTPFFMFSDALHVNFITPWVRPPVEVYSLNPFSSVDGVDALY
                     RTEAGPMDIEVQGVFGKSSLGIHGGTVKLRAIRTFNVAASGHGLTLHAGYTTANLDVH
                     WGDQPSQNLLAALNATGFGAIADELNGTGAKASFTSAGFQYDRDHWLLIGEFARRDAS
                     HFISSAQGWYFTAGYRVGSVTPYVTYARQLETAPVTSSTVPIPALQTGLDLFNTSRRN
                     AQRTEGIGVRWDFARNFALKGQFDLIRPGPDGLGIFVSPSAGYLTPSGPIHVLSLSVD
                     VVF"
     gene            5006..5431
                     /locus_tag="PLH85_15385"
     CDS             5006..5431
                     /locus_tag="PLH85_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66001.1"
                     /translation="MSRRLSALLIALFALGTGWPAHAELVIVAGPRSAITELTREHAE
                     KLYLGRATTLTDGTPVTLMDLPVGPARDDFYLKLAGKNSTQIRAYWSRLVFTGRAMPP
                     READSIAEARQWLADIPNLIGYLDKGDVTPGMRVLLRIP"
     gene            5550..6362
                     /locus_tag="PLH85_15390"
     CDS             5550..6362
                     /locus_tag="PLH85_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB66002.1"
                     /translation="MKTASSQQAYRPYPSFSTAQTLLWFIGGVLLMFVLVSAAFAIKL
                     GVEGRLPFVRRTVDAPRADAATPPPTPAARPTGQNFSSGAETILPPEVSHLAKTTAAP
                     DTAPARPTEGPAPSAGTPGAATPASAPVTPAPVTPAAAPAPVAAQAPATPPVRPVDPT
                     GDVTEALHAWVTAWARRDVPTYLEHYAQDFRPAAGLSRSAWQSQRRQRLSRGGELLIS
                     LSDVQVQVSGDKATARFVQSYRSDTLTSTEPKTLELIRRDGHWLILSEQTGS"
     gene            6449..6760
                     /gene="rplU"
                     /locus_tag="PLH85_15395"
     CDS             6449..6760
                     /gene="rplU"
                     /locus_tag="PLH85_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNB66003.1"
                     /translation="MYAVIKTGGKQYRVVAGEKLKVEQIPADVGSQITLDQVFMVGEG
                     ESVKIGTPVVAGATVTATVLSHGRHDKVKIFKMRRRKHYQKHQGHRQNYTELRIEAIS
                     A"
     gene            6782..7045
                     /gene="rpmA"
                     /locus_tag="PLH85_15400"
     CDS             6782..7045
                     /gene="rpmA"
                     /locus_tag="PLH85_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNB66004.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGVKRYGGQFVLAGNIIVRQRGTE
                     YHPGENVGIGKDHTLFALTEGTVQFTVKGANKRRTVSIVAAAE"
     gene            7223..8362
                     /gene="obgE"
                     /locus_tag="PLH85_15405"
     CDS             7223..8362
                     /gene="obgE"
                     /locus_tag="PLH85_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNB66005.1"
                     /translation="MKFFDEARIDVIAGDGGNGAATFRREKYIPKGGPDGGDGGKGGD
                     VYAIADRNLNTLIDYRYKRTFTAERGENGRGADCYGKGGEDIELRMPVGTVISEQESG
                     EVIADLDHDGKRVMIARGGRGGLGNLHFKTSTNRAPRKKTMGEAGERKSLNLELKVLA
                     DVGLLGMPNAGKSTFIRAVSSARPKVADYPFTTLAPNLGVVRTSENRSFVIADIPGLI
                     EGAADGAGLGHQFLRHLQRTHILLHIVDIAPFDPEADPVRDAHAIVEELYKYDESLAA
                     KRRWLVLNKLDLIPDAEERAARVQDFLAAYGPVERHFQVAAISGERCQEVVFAIQDLL
                     DAEKAAAQAAADEAARAAAAHAADADAALDDTDIDHPDSDESSPA"
     gene            8359..9477
                     /gene="proB"
                     /locus_tag="PLH85_15410"
     CDS             8359..9477
                     /gene="proB"
                     /locus_tag="PLH85_15410"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNB66006.1"
                     /translation="MRTRIQNARRLVVKVGSALVTNNGAGLAVEALAEWARQIARLRA
                     DGREVVLVSSGAIAAGMQRLGWSKRPQEMHHLQAAAAVGQMGLAQAYESVFARNDLHT
                     AQILLTHADLADRTRYLNARSTLVTLLELGVIPIINENDTVVTDEIKFGDNDTLGALV
                     ANLIEADALIILTDQQGLYTADPRNDPSATLISEERAENTALEAMAGGAGSGISKGGM
                     ITKVRAAQRAARSGAHTCIASGREPDALLRLVGGDDLGTLLYASSTPLAARKQWLADH
                     LQLAGSLILDDGAVDALRAGRSLLPVGVTAVEGDFNRGAVVACRTASGIELARGLINY
                     ASSDARRIARSPSSEIEHLLGFAEAPELIHRDNLVLIG"
BASE COUNT         1767 a         3663 c         2817 g         1454 t
ORIGIN      
        1 atcgacatgg cctcgcccaa catcaacctg cgcgacccgg ccatctaccg catccgccac
       61 gccacccacc accgcaccgg cgaccgctgg tgcgtgtacc cgatgtacac ctacgcgcac
      121 cccatcgagg acgcgctgga ggacatcacc cactccatct gcaccctcga attcgaagac
      181 cagcgcccct tctacgactg gctgctggac gccctcgccg acggcggttt cttcccgcgg
      241 cccctgcccc agcagatcga attcgcccgc ctcaacctca cctacgtggt gctgtccaag
      301 cgcaagctga tccagctcgt ggacgaaggc cacgtggccg gctgggacga ccctcgcctg
      361 cccacgctcg tcggcgcccg ccgccgcggc ttcacgccgg aaggtttccg ccagttcgcc
      421 gagcgcatcg gcgtctccaa ggccgattcc tggatcgaca tgagcgtcct cgaagactgc
      481 atgcgcgagc atctcaacga agcggccgaa cgccgcgtcg ccgtgctcga cccggtcaaa
      541 ctgatcatca ccaactaccc cgagggccag gtcgagcgct gccacgcgcc gaaccacccc
      601 ctcaagccgg agctgggcaa gcgcgagatg cccttcggcc gcgaactgtg gatcgagcgc
      661 gaggacttca tggagacccc ggtcaagggt taccaccgcc tctatcccgg taacaccgca
      721 cggctacgtt acggctatgt cgtccgctgc accggctgcg agaaggacgc cgacggcaag
      781 gtgaccgccg tgctgtgcga gtacttcccg gattccaagt ccggtacgcc cggcgccgac
      841 acctacaagg tcaagggcaa cctgcactgg gtcgcggtgg cacacgccta cgccgccgaa
      901 gtgcgcctct acgaccggct gttcgcccac ccctatccgg gcacccgtcg cgaaggcgat
      961 cccgaggacc tgcagcggga cttcctcgac gacatcaacc cggacagcct gcgcgtcatc
     1021 cgcgcccagc tcgaaccggc cctgcgcgcc gcacgtccgg aagcccgctt ccagttcgaa
     1081 cggcacggct acttcgtcgc cgaccgcatc gacacacagg aaggcgctcc ggtgttcaat
     1141 cgcacggtga ccctgcgcga cgcatggaag cacggcgcat gaacagtgga taatccgtga
     1201 agtttccggc gacagcttgc ccgcctcggc gggcgtcgcc gcaaggcgcg gcgttcaagc
     1261 ttcgggcgat gctgccgatg tatgtcggac cctgccccgc cagccctccc catccgttgc
     1321 gcggtgcccg gatggagcga cacacccggt cgagcccctg aacctgtcca tgctgagcaa
     1381 cctctcgatc cgcgtccgac tcctgctggc gagcaccgtc gtccaggtgg tgatgctcac
     1441 cctgctgctc accaacagcg cgcggctcat gaacgaagcc accggtgcca gcctggaaac
     1501 cctgatctcc cagaacgccg cgatcctcaa catcgtcacc gccagcttcg tcccccaggg
     1561 gcgctacgcc gagcttcagg acgccctcgg cgaactcctc aatgacagca gcgaaggcct
     1621 cgtctacgta cgcatcgtgg atgccagcgg ccatacccag gtgcgcgccg gcatgccgga
     1681 tctggcgacc ctgccccccc ccgacaatcc cgccaccctg accctcccgg cgggggaatc
     1741 acgcaacgtc atcaacatcc ggcggccgct cctgatggac cgtaaccagg tgggcttcct
     1801 ccagttcggc gtctcggtgt cggcgctgac cgtcgccaag cagcagatcc tcagccaggg
     1861 gctgttcatc gccactgccg aggtgctcct caccctgctc ctgctgggca ccatcggcta
     1921 cctgatgacc cgcaacctgg gccgcctgct gcggggcagt caggccatcg cggccggcga
     1981 cctcagccat cgcattccgg aaaagggtca ggacgagctc gcccagctgt gcaagcactt
     2041 caaccgcatg gccctcgccc tgcagtcgcg catccatgag ctggaagcca ccgcgcgcca
     2101 gctcgcccag agcgaagagc ggtacgccct ggcgacccgc gccgccaacg acggcctgtg
     2161 ggactgggac atcgtcaccg gcaagactta cttcgcgccg cgctttgccg agatcctcgg
     2221 catcacgccc gacgccttcg tgggcgaccg ggacaccctc ttcaaacaca tccatccgga
     2281 agaccgggag gccttccgcc tgcgcctggt ggcccacctc aagcgcgaga cggcccagct
     2341 caacgtcgaa caccgcatcc gccacgccga cggcagctat cgctggaccc tcacctgcgg
     2401 catggcggtc tacgaccccg acagcggccg cgccatccgc atggccggct ccatcagcga
     2461 catccacctg cgccgccggg ccgaagacca gctcgtgcat gacgccctgc acgacggtct
     2521 caccggtctc gccaaccgcg ccctctttgt ggaacacctg cagaacaccc tgcgccatgc
     2581 cgaccgggtc acgggcagcc tgttcgcggt gctgcacgtc aatatcgaac gcttccacgt
     2641 ggtcaacgac agcctcggct actcggcagg tgacgacctg ctgcgcctcg tgggtcagcg
     2701 catccaggcc ctcgcgcggc ccggcgacgt ggtcggacgg gtcggcggcg accagttcgc
     2761 cctgctcctc aacgatatcg ccgatgccag cgaagcgatc cgcttttcgg aaaacctcca
     2821 gacccgcctc gccgagagcg ccgaactcaa cggccattcg ctctacccca agacccgcat
     2881 cggcgtggcc ctgggcagcg gcggcttcca caatgccgaa gacctgatgc gcgatgcgga
     2941 caacgccatg caccgctcgc gcagcagctc cgacagcacc atcacggtct tccagtccac
     3001 catgcaccag caggccctgc gcgacctgca actggaatcc cagttgcgca tcgccctgca
     3061 ggaaggccag ttcgtcgccc actaccagcc catcgtgcat ctggccgacg gccgcacagc
     3121 aggcttcgag gccctggtcc gctggcccga acaggcaacc cgacggatcg gcccggacgt
     3181 cttcgtcccc cttgccgaat ccctcgatct gatccacgaa ctgggcatgc acatgctcga
     3241 ccagagctgc gcccagctca aggcctggcg cacactcggc cttgcagacg gcgatctgag
     3301 catcagcgtc aacctttccg cccgtcagct gatgcaggtg gatctcgccg accaggtcgt
     3361 cgagtgcatt gaacgccacg aactcccccc cagcgccctc aagctggaaa tcaccgaggg
     3421 caccctggtc acacgcaagg agctcgccac cagccagctc ggcaagctgc gtgccaaggg
     3481 tctcaagatc ctcatcgacg acttcggaac cggctactcg tccctgagct atctacacac
     3541 cattccctgc gacacactca agctcgacgg ctccttcatc cgtgagatcg aacgggatac
     3601 acggctgcgc gccattgtcg gcgccaccat ccgcctgtcc cacgagctcg ggatcagcgt
     3661 cgtcgcggaa tgtatcgaaa cgcccgccca ggcccaactg ctgcgcgacc tggggtgtga
     3721 ctacggtcag ggctacctgt tttcctcggc ccagccgggc gacaaggcgc aacaatggct
     3781 gctcaacaac cttcccgtcg gtgccggggc atgaagcgca tcgccgccct tctgatcctc
     3841 ggagcaagcc tcccggtcgc cgccgacgaa ccctcgcgtt tcagtttctc cggattcggc
     3901 acggtgggag ccgtccggac gagcaatgat gtgctgggct ttcgcagcag cgtcgtgcag
     3961 gacacagggc ccggacgggc ctttgatacc tccgtcgaca gcgtgatcgg cgtgcaggga
     4021 tccgcccacc tgaccggcaa gtccgacatc accctgcagg tcgtctccca caaaggcccg
     4081 gacgacgcct tcacgcccca ggtgacctgg agcttcctcc gtacccagct cctgccggag
     4141 ctgagcgtcc gggccgggcg catgcggacg ccgttcttca tgttttcgga cgcgctgcac
     4201 gtcaatttca tcacgccgtg ggtccgcccg ccggtcgagg tctacagcct caaccccttc
     4261 tccagcgtcg atggcgtgga tgccttgtac cgcaccgagg ccggccccat ggacatcgag
     4321 gtccagggtg tgttcgggaa gtccagcctg ggcatccatg gcggtaccgt caagctgcgc
     4381 gccatccgca ccttcaatgt cgccgccagc ggccacggac tcaccctgca cgccggctac
     4441 accacagcca acctcgacgt gcactggggc gaccagccct cccagaacct cctcgcggcc
     4501 ctcaatgcca ccggcttcgg cgctatcgcc gatgaactca acggaaccgg cgcgaaggcg
     4561 agcttcacgt ccgccggctt ccagtacgat cgcgaccact ggctgctcat tggcgaattt
     4621 gcccgtcgcg atgccagcca tttcatcagc agcgcgcagg gctggtattt cactgcaggc
     4681 tatcgggtgg gcagcgtcac cccctatgtc acctacgccc gccagctcga gaccgccccg
     4741 gtgaccagca gcacggtccc cattcccgcc ctgcagaccg gtctggatct cttcaacacc
     4801 tcccgacgca acgcccagcg caccgaaggc atcggcgtcc gctgggattt cgcccgcaac
     4861 ttcgccttga aagggcagtt cgacctcatc cggcccggcc ccgacggcct cggcatcttc
     4921 gtcagtcctt ccgccggcta cctcacgccc agcgggccga ttcacgtact gagcctgtcc
     4981 gtcgatgtcg ttttttaagc cccccatgag tagacggctc tcagcgctcc tcatcgccct
     5041 gttcgcgctg gggacgggat ggcctgccca cgcagaactg gtgatcgtcg caggcccgcg
     5101 cagcgccatc acggagctca cccgggagca tgccgaaaaa ctttacctcg gccgtgccac
     5161 caccctgacc gacggcacgc ctgtcaccct catggacctc cccgtcggac cggcgcggga
     5221 cgatttctac ctcaaactgg ccggcaagaa ctccacccag atccgcgcct actggtcccg
     5281 gctcgtcttc acgggccgcg ccatgccccc ccgcgaagcg gattccatcg ccgaggcgcg
     5341 ccaatggctg gcggacatcc ccaacctcat cggctacctg gacaagggcg acgtcacgcc
     5401 aggaatgcgc gttttgctgc gcatcccgtg acacttccga cgcccaattg caaacatacg
     5461 tagaacttga gcgctgagcg gccgcgtgcc gcattgcttt gcacgaggat ttcctagaat
     5521 ggtccggatt gaacgattga gtccgtacca tgaagactgc ctcctcccag caggcctacc
     5581 gcccttaccc gagtttcagc acggcccaga cgctgctgtg gttcatcggc ggggttctgc
     5641 tgatgtttgt cctggtgtct gccgccttcg ccatcaagct tggcgtcgaa ggccggctgc
     5701 ccttcgtccg ccggacggtc gacgctccgc gcgccgatgc cgcaacgccg ccgccgacac
     5761 ccgccgcgcg tcccacaggc cagaactttt cgagtggcgc cgagacgatc ctgccgcccg
     5821 aagtctcaca cctcgcaaag acgaccgcgg cccccgacac cgcgccggcg cgcccgactg
     5881 aaggtcccgc accaagcgcc ggaacgcctg gcgccgcgac gccggcatct gccccggtga
     5941 cgcctgcccc ggtgacgcct gccgcggcac ccgcccccgt cgcagcacag gctccggcaa
     6001 cgcccccggt gcgcccggtg gatcccaccg gcgacgtcac cgaggccctg cacgcctggg
     6061 tgaccgcctg ggcacgtcgc gacgtcccca cgtatctgga acactatgcg caggacttca
     6121 gacccgcggc aggcctgagc cgctccgcct ggcagagcca gcgtcgccag cgtctgtccc
     6181 gtggcggcga gttgctcatc agcctgtccg acgtccaggt gcaggtcagc ggcgacaagg
     6241 ccacggcccg ctttgtccaa agctaccgct cggacaccct cacctcgacc gaacccaaga
     6301 ccctggagtt gatccgccgg gacggccact ggctgatcct cagcgaacag accggcagct
     6361 gaaggacgca caaatcgggg tttgacgcaa gcgtttcctt gccctatact gctgcgtttc
     6421 ctgcaaaccc tttaatcgga gtacatccat gtacgcggtc ataaaaaccg gcggcaagca
     6481 gtatcgtgtc gtcgccggcg aaaaactcaa agtagaacag ataccggctg acgtgggctc
     6541 gcaaatcacc ctcgaccagg tcttcatggt cggcgaaggc gagtcggtca agatcggcac
     6601 ccccgtggtg gccggcgcca ccgtgacggc cactgtgctg tcccacggcc gccacgacaa
     6661 ggtcaagatc ttcaagatgc gccgccgcaa gcactaccag aagcaccagg ggcatcgcca
     6721 gaactacacc gagcttcgca tcgaagcgat ttcggcctaa gcggcccaag gagaaaccga
     6781 catggcacac aaaaaggctg gcggcagttc ccgtaacggt cgcgactcag aaagcaaacg
     6841 cctgggcgtc aagcgctacg gtggccagtt cgtcctcgcc ggcaacatca tcgttcgcca
     6901 gcgcggcacc gaataccatc cgggcgaaaa cgtcggcatc ggcaaggacc acaccctgtt
     6961 cgcgctgacc gaaggcaccg tgcagttcac cgtcaagggc gccaacaagc gccgcaccgt
     7021 gagcatcgtc gctgccgccg aataagcgca gcacacggtt tgccaaagcc ctatcgtgcg
     7081 atagggcttt tttcattcag gggacgcacg ttcgcggcgc cgaccacagg ccccaccgca
     7141 gcgctgaccg agcaccggat ccgcatgccg cttcgccggc aaggccccac cgctcgtcgc
     7201 ccatcaccca ccggatacgc ccatgaaatt cttcgacgaa gcccgtatcg acgtcatcgc
     7261 cggcgacgga ggcaatggcg cggccacctt ccgtcgcgaa aagtacattc ccaagggcgg
     7321 accggacggc ggtgacggcg gcaagggcgg cgacgtttac gccatcgccg accgcaacct
     7381 gaacaccctc atcgactacc gctacaagcg caccttcacc gccgagcgcg gcgagaacgg
     7441 acgcggcgcc gactgctacg gcaaaggcgg cgaggacatc gagctgcgca tgccggtggg
     7501 cacggtcatt tcggaacagg agagcggcga agtcatcgcc gacctggacc acgacggcaa
     7561 gcgcgtcatg atcgcccgcg gcggacgcgg cggtctcggc aacctgcact tcaagaccag
     7621 caccaaccgc gccccgcgca agaagaccat gggcgaggcc ggcgagcgca agagcctcaa
     7681 cctggagctc aaggtgctcg ccgacgtggg cctgctgggc atgcccaacg cgggcaaatc
     7741 caccttcatc cgcgccgtgt cctcggcccg ccccaaggtg gcggactacc ctttcacgac
     7801 cctcgccccg aacctgggcg tggtgcgcac ctcagagaac cgcagcttcg tcatcgccga
     7861 cattcccggg ctgatcgaag gcgccgccga cggcgccggc ctcggtcacc agttcctgcg
     7921 ccacctgcag cgcacccaca tcctgctcca catcgtggac atcgccccct tcgatcccga
     7981 agcggacccg gtgcgtgacg ctcacgccat cgtcgaggag ctgtacaagt acgacgagag
     8041 cctcgccgcc aagcgtcgct ggctggtcct caacaagctc gacctgattc ccgatgcgga
     8101 ggaacgcgcc gcccgcgtgc aggacttcct cgccgcctac gggccggtcg agcgccactt
     8161 ccaggtcgcg gccatcagtg gcgagcgctg ccaggaagtg gtcttcgcga tccaggacct
     8221 gctcgacgcc gaaaaagccg ccgcccaggc tgccgccgac gaggccgccc gcgcggcagc
     8281 ggcccatgcc gcggacgcgg atgccgcgct tgacgacaca gacatcgacc accccgacag
     8341 cgacgaaagc agccccgcat gagaacccgc atccagaacg cccgccgcct ggtcgtcaag
     8401 gtcggcagcg ccctcgtcac caacaacggc gccggcctcg cagtcgaagc gctggccgaa
     8461 tgggcccgcc agatcgcccg cctgcgggcc gacggccgcg aagtggtcct ggtgtcgtcc
     8521 ggcgccatcg ccgccggcat gcaacgcctc ggctggagca agcgccccca ggaaatgcac
     8581 cacctgcagg ccgccgccgc ggtcggccag atgggcctcg cccaggccta tgaatccgtg
     8641 ttcgcccgga acgacctgca caccgcccag atcctgctca cccatgccga cctggcggac
     8701 cgcacccgct acctcaacgc ccgctcgacg ctggtgaccc tgctggagct gggcgtcatt
     8761 cccatcatca acgagaacga caccgtcgtc accgacgaga tcaagttcgg cgacaacgat
     8821 accctcggcg ccctcgtcgc caacctgatc gaagccgacg ccctgatcat cctcaccgac
     8881 cagcagggcc tgtacaccgc cgatccgcgc aacgatccga gtgccaccct gatctccgag
     8941 gagcgcgccg agaacaccgc cctcgaagcc atggccggcg gcgccggcag tggcatcagc
     9001 aagggcggca tgatcaccaa ggtccgcgcc gcccaacgcg ccgcccgcag cggcgcccac
     9061 acctgcatcg ccagcggccg cgagcccgac gcgctgctcc gccttgtcgg tggagatgac
     9121 ctcggcaccc tgctgtacgc cagcagcaca ccgctggcgg cccgcaagca gtggctcgcc
     9181 gatcacctgc agctggccgg cagcctgatc ctcgacgacg gcgcggtgga cgccctgcgg
     9241 gccgggcgca gcctgctgcc ggtaggcgtc accgccgtgg aaggcgactt caaccggggg
     9301 gccgtggtgg cctgccgcac cgcgagcggc atcgaacttg cgcgcgggct gatcaactac
     9361 gccagctccg acgcacgccg catcgcccgc agtccatcca gcgaaatcga gcacctgctg
     9421 ggctttgccg aagcgccgga actgatccac cgggacaatc tggtgctgat cggctgaagg
     9481 cgcggacgga cccagcaaaa aaggcgacct gaggtcgcct tttttacatg tttcggtgcg
     9541 ccgtgcgttc cggcgccgaa ccacctccag ccatgcgcgg ggccgtcgtc cacgaaaaaa
     9601 acgggtgccc gaaggcaccc gtttgatttc caaatccgaa cgagccggaa ttagaaggtg
     9661 tgacgcagac cgacggacac gccggacacg gtggagccag c
//
LOCUS       DAOQMM010000126         9244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3663_length_9244_cov_49.365981, whole genome shotgun sequence.
ACCESSION   DAOQMM010000126 DAOQMM010000000
VERSION     DAOQMM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3663_length_9244_cov_49.365981"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="PLH85_15415"
     CDS             complement(<1..1621)
                     /locus_tag="PLH85_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HNB66007.1"
                     /translation="MIGRIAPGARAAAGSPEELRRYDLLLAGFDLLDQAIAVFDAAPR
                     LVTWNTAMLRLLDFPEELVRVGTPFEDFVRFNVERGEYGPGETERLIAERMAAVRAFQ
                     PHQAERTRPNGQILAIRGVPIPNLGFVTLWTDVTEQRRYEKLIEGQNAELETRVRART
                     AELEAANRDLTEAKSRLDSVAAQLSRSEERLRLIMDAIPALIAYVDAGERYRFANRGY
                     AQWFGLDKNAIVGRSIAEVFGPDAYAQIKPHLETAADGERVSYEYTRTDASGRQVFAR
                     SAVVPDTSEAGGVQGYFVLSIDITEQKASQAALIQAQKMEAVGQLTGGLAHDFNNLLT
                     IITGNLHALRDKLPAHQGYDDYLEPALSAAGRGTELIRRLLSFSRQQPLEPRAIEVGV
                     LVTGMGQLLSRTLTEHIRLQLDLPTRKLHVLADPHQLENALLNLALNARDAMPDGGEL
                     RIAVAPRHISASLSLLVEVPAGEYVQFDVSDTGTGIDPALLPRLFEPFFTTKRFGAGS
                     GLGLAMVYGFVRQSGGNIRILSTPGKGTNVRF"
     gene            complement(1618..3729)
                     /locus_tag="PLH85_15420"
     CDS             complement(1618..3729)
                     /locus_tag="PLH85_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNB66008.1"
                     /translation="MSDLLPEHLKAEAVQLLEGLLANALIGIYLIQDGLFRHVNSQFC
                     ETFGYAAAEICDRLGPEDLVFPDDVERVRQTLADRIEGRQAAARYTFHGRHRSGRQLV
                     VEVFGLRIDVAGRPAVVGVLSDITARHVAERATAEQLGFIAQLIEAIPSPLFFKDAEA
                     RYLGCNQAFERFIGMAREAIIGKSVFEISPPDLAVRYHAADKALFDNPGVQTYEASVE
                     SSDGGRRDVLFNKATFLRGDGSMGGLVGVITDITERKRTEALIWAQANYDPLTGLPNR
                     RLLNDRLIEHVKRADRDGSSVALLFIDLDRFKEVNDSLGHAAGDLLLEEAARRILACV
                     RETDTVARQGGDEFAVLLPGLHEHGPIERIAEAILRILGEPFAVGADECFVSASIGIT
                     LYPRDATSPADLFKNADQAMYHAKDNGRSRFSYFSPALQEAALHHLQLGRDLRQAIAG
                     HQLRVEYQPIYHLASGALLKAEALLRWTHPTRGEIGPAEFIPTAEEIGLIDEIGDWVF
                     ARVTETLLRWRATLAPGDAERCPLQITVNTSPRQFTGGRSVEAQLDRMDAAGLPGACI
                     ALEITESLLLGAQPRVADRLARLSARGVQVSLDDFGTGYSAMAYLKRLDIDTLKIDRS
                     FVRDLTTDSSDLAIAEAIIAMAHKLGLDVVAEGIETDEQRRILAATGCDFGQGYLFAR
                     PLSEDALLTLLEGQRRALATP"
     gene            complement(3770..4858)
                     /locus_tag="PLH85_15425"
     CDS             complement(3770..4858)
                     /locus_tag="PLH85_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB66009.1"
                     /translation="MPMSSSSAAPAPSPAAPERRRASVAMGFVTGMVAGMQRRGHDPA
                     PLLAATDIDLADSASRIPLDRYAALYNRVIRDLDDEGFGLFERPLGTGSFEFLCRGML
                     GAPHLAEALERARRFLHIVLPDLAVSIHRLDSHAELRIAETRPLCPTRDDPARVFAFE
                     WLLRLLHGLACWLVGRGLALDAVRFPFARPPHADDYQLIYTAHFSFGGDDLAARFNPT
                     LLDLPIRRDEAALARFLEGAPGKITTLYRRDRDTVVRVRDLLRAALPANLSQEHVADR
                     LHLSPRTLHRRLDEEGSSFRAIKEALRRDIAVARLTKTRHSIARVAADLGYADTSAFY
                     RAFIAWTGLSPERYRKQLAAQAPAQSAP"
     gene            4993..8259
                     /locus_tag="PLH85_15430"
     CDS             4993..8259
                     /locus_tag="PLH85_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB66010.1"
                     /translation="MTDLSVAKKLVTYKPKNKVRFVTAAALFDGHDASINIMRRILQS
                     TGCEVIHLGHNRSVGEIVNAALQEDVQGIAITSYQGGHVEFFKYMIDLLREHGGENIR
                     VFGGGGGVIVPAEIKELHDYGVTRIYSPEDGAILGLQGMINDVVERSDMDLSHAGPQG
                     ADAVLAALRSGDRRALARIITGLENGAYADEVKTALIAAAKELKTPTLGITGTGGAGK
                     SSLTDELVRRFRLDQHDRLKLAIVSIDPSRKRTGGALLGDRIRMNAIEHPNIFMRSLA
                     TRDTGKEVSVALPEVIAATKLAGFDLVIVETSGIGQGDAAIVPFVDVSLYVMTPEFGA
                     ASQLEKIDMLDFADFVAINKFDRKGAEDALRDVRKQVQRNRELFTTQVEQMPVFGTMA
                     ARFNDDGVTALYQALAPALVAKGLKLGKPGLPLVSVRASSRGRAIVPAERARYLAEIA
                     DAVRGYHKHTLEQSRIARERQALRISKRLFEGCQKDAGSFDELIDWKDGELTPSSRKL
                     LDMWPKTVELYAADEYVVKIRDKEIRTALTSESLSGSKIRKVALPSFEDDGESLRFLM
                     KENVPGSFPYTAGVFAFKREGEDPTRMFAGEGDAFRTNRRFKKVSEGMPAHRLSTAFD
                     SVTLYGCDPDPRPDIYGKVGNSGVSIATLDDMKVLYDGFDLVNPTTSVSMTINGPAPI
                     ILAMFFNTALAQQLEKFKAQNGREPTDDEAQKIREWVLATVRGTVQADILKEDQGQNT
                     CIFSTEFALKMMGDIQEFFVHNKVQNFYSVSISGYHIAEAGANPISQLAFTLSNGFTY
                     VESYLARGMHIDDFAPNLSFFFSNGMDPEYSVIGRVARRIWAVAMKNKYGANERSQKL
                     KYHIQTSGRSLHAQEMDFNDIRTTLQALIAIYDNCNSLHTNAYDEAITTPTEESVRRA
                     MAIQLIINREWGLAKNENPNQGAFIIEELTDLVEEAVLKEFEAISARGGVLGAMETGF
                     QRGKIQEESLYYEHQKHDGSYPIIGVNTFLNPKGMAQQEIELARSSEDEKQSQLQRLA
                     AFQDRNSDEAPQWLAKLKQTVIENGNVFAVLVDAVKYCSLGQITTALYDVGGQYRRSM
                     "
     gene            8436..8663
                     /locus_tag="PLH85_15435"
     CDS             8436..8663
                     /locus_tag="PLH85_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNB66011.1"
                     /translation="MHTTIGAGDFKARCLKLLDDVARSGEALVITKHGKPVAKLVPMP
                     PAGSLFGALAGSVRQEGDIVAPVDDDWDACR"
     gene            8660..9079
                     /locus_tag="PLH85_15440"
     CDS             8660..9079
                     /locus_tag="PLH85_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB66012.1"
                     /translation="MSPLLLDTHVLVWLLAGSDRLGLRARAAIDAAAAVEGVFVSAIT
                     PWEIAMLVAKGRLVLERDVGDWVQAALALPGIRLEALSPAVAVASTRLPGALHADPTD
                     RIIVATARHLGAALVTADGLLLDYASSGNLKVLPAGQ"
BASE COUNT         1508 a         3036 c         3290 g         1410 t
ORIGIN      
        1 tgaagcgcac gttggtgccc ttgcccggcg tgctgaggat gcggatgttg ccgccggact
       61 ggcgcacgaa gccatacacc atggccagcc cgaggccgct gccggcaccg aagcgcttgg
      121 tggtgaagaa gggctcgaag aggcgcggca gcagggcggg atcgatgccg gtgccggtat
      181 ccgagacgtc gaactggacg tattcgccgg ccggcacctc caccagcagg gacaggctgg
      241 cgctgatgtg gcgcggggcc acggcgatgc gcagctcgcc gccgtcgggc atggcgtcgc
      301 gggcgttgag ggccaggttg aggagcgcgt tctcgagctg gtggggatcg gccaggacgt
      361 ggagcttgcg ggtgggcagg tcgagctgca ggcggatgtg ttcggtgagg gtgcgggaca
      421 ggagctgacc catgcccgtc accaggacgc ccacctcgat ggcgcgaggt tccaggggct
      481 gctggcggga gaaactgagc aggcggcgga tcagctcggt gccgcgcccg gcggcggaca
      541 gggcgggctc gagatagtcg tcgtagccct ggtgggccgg cagcttgtcg cgcagggcgt
      601 ggaggttgcc ggtgatgatg gtgagcaggt tgttgaagtc gtgggcgagg ccgccggtga
      661 gctggccgac ggcttccatc ttctgggcct ggatgagggc ggcctgactg gctttctgct
      721 cggtgatgtc gatggacagg acgaagtagc cctgtacgcc accggcctcc gaggtgtcgg
      781 gcaccaccgc gctgcgggca aagacctggc ggccgctggc gtcggtgcgg gtgtattcgt
      841 agctgacgcg ctcgccatcg gcggcggtct cgagatgggg cttgatctgg gcgtaggcgt
      901 cgggcccgaa gacctcggcg atgctgcgcc cgacgatggc gttcttgtcg agcccgaacc
      961 actgggcgta gccccggttg gcgaagcggt agcgctcgcc ggcgtccacg taggcgatca
     1021 gggccgggat ggcgtccatg atgaggcgca ggcgctcctc gctgcggctc agctgggcgg
     1081 cgacgctgtc gaggcggctc ttggcctcgg tgaggtcccg gttggcggct tccagctcgg
     1141 cggtgcgggc gcggacgcgg gtctccagct cggcgttctg gccttcgatg agcttttcgt
     1201 agcggcgctg ctcggtgaca tcggtccaca gggtgacgaa gcccaggttg gggatgggca
     1261 cgccgcggat ggccaggatc tggccgttgg gccgggtgcg ctcggcctgg tggggctgga
     1321 aggcgcgcac ggcagccatg cgttcggcga tgaggcgttc ggtctcgccg gggccgtact
     1381 cgccgcgctc cacgttgaag cgcacgaagt cctcgaaggg ggtgccgacg cgcaccagtt
     1441 cttccgggaa gtccagcagg cgcagcatcg cggtgttcca ggtgaccaga cgcggagcgg
     1501 cgtcgaagac ggcgatggcc tggtccagga ggtcgaagcc ggcgagcagc aggtcgtagc
     1561 ggcgcagttc ctccggcgag ccggcggcgg cccgcgcgcc tggggcgatg cggccgatca
     1621 tggggtggcg agggcccggc gctggccttc cagcagggtg aggagggcat cctcggagag
     1681 cggccgggca aacaggtaac cctggccgaa gtcacaaccg gtggcggcga ggatgcggcg
     1741 ctgctcgtcg gtttcgatgc cctcggcgac cacgtccagg ccgagcttgt gggccatggc
     1801 gatgatcgcc tcggcgatgg ccaggtcgct ggagtcggtg gtgaggtcgc gcacgaagga
     1861 gcggtcgatc ttgagggtgt cgatgtccag gcgcttcagg taggccatgg ccgagtagcc
     1921 ggtgccgaaa tcatccaggg agacctgcac gccccgcgcg ctgaggcggg cgaggcggtc
     1981 ggcgacccgc ggctgggcgc ccagcagcag gctctcggtg atctccaggg cgatgcaggc
     2041 gccgggcagg ccggccgcgt ccatgcggtc gagctgggct tccaccgagc ggcccccggt
     2101 gaattgccgc ggggaggtgt tgacggtgat ctgcagcggg cagcgttcgg cgtcgccggg
     2161 ggccagggtg gcgcgccagc gcagcagggt ctcggtgacg cgggcgaaca cccagtcgcc
     2221 gatctcgtca atgaggccga tttcctctgc ggtggggatg aactcggccg gcccgatctc
     2281 gccgcgggtc gggtgggtcc agcgcagcag ggcctcggcc ttgagcaggg cgccgctggc
     2341 cagatggtag atgggctggt actcgacgcg cagctggtgg ccggcgatgg cctgacgcag
     2401 gtcgcggccc agttgcaggt gatgcagggc cgcctcctgc agggccggcg agaagtagct
     2461 gaagcggctg cgcccgttgt ccttggcgtg gtacatggcc tggtcggcgt tcttgaacag
     2521 gtcggcgggg gaggtggcgt cgcgcgggta gagggtgatg ccgatgctgg ccgatacgaa
     2581 gcattcgtcg gcgcccaccg cgaagggctc acccaggatg cggaggatcg cctcggcgat
     2641 gcgttcgatg gggccgtgtt cgtgcaggcc cggcagcagc accgcgaatt cgtcgccacc
     2701 ctggcgcgcc accgtgtcgg tctcgcgcac gcaggccagg atgcggcggg cggcttcttc
     2761 gagcagcagg tcgccggcgg cgtggccgag ggagtcgttg acctccttga agcgatccag
     2821 gtcgatgaac agcagggcca cgctgctgcc gtcccggtcg gcgcgcttga cgtgttcgat
     2881 gaggcggtcg ttgagcagcc gccggttggg caggccggtg agggggtcgt agttggcctg
     2941 ggcccagatc agggcttcgg tgcgcttgcg ctcggtgatg tcggtgatca cgcccaccag
     3001 gccgcccatg ctgccgtcgc cgcgcaggaa ggtggccttg ttgaagagca cgtcgcggcg
     3061 cccgccatcg gaggattcga cgctggcctc gtaggtctgc acgccgggat tgtcgaacag
     3121 ggccttgtcg gcggcgtggt agcggacggc caggtcgggg ggcgagatct cgaagacgga
     3181 cttgccgatg atggcttccc gggccatccc gatgaagcgc tcgaaggcct ggttgcagcc
     3241 cagatagcgg gcttcggcat ccttgaagaa gagcgggctg ggaatcgctt cgatgagctg
     3301 ggcgatgaag cccagctgtt cggcggtcgc gcgttcggcc acgtggcgcg ccgtgatgtc
     3361 cgagagcacg cccaccacgg ccgggcggcc ggcgacgtcg atgcgcagac cgaagacttc
     3421 gacgaccagc tggcggccgc tgcgatggcg tccgtggaag gtgtagcgtg ccgcagcctg
     3481 gcggccttcg atgcggtcgg cgagggtctg gcggacccgc tccacgtcgt ccggaaacac
     3541 cagatcctcc gggccgaggc ggtcgcagat ctccgccgcc gcgtagccga aggtctcgca
     3601 gaactggctg ttcacgtggc ggaacaggcc gtcctggatc aggtagatgc cgatcagggc
     3661 attcgccagc agtccctcca gcaactggac ggcctctgct ttcagatgct cgggcagcaa
     3721 atcggacatg gggtcgggcg cgcggatgat cggtgcattg taagccgaat cacggcgcac
     3781 tctgtgccgg ggcctgggcg gcgagctgct tgcggtagcg ctccggcgac aggccggtcc
     3841 acgcgatgaa ggcccggtag aacgcggaag tgtcggcgta gcccaggtcg gcggcgacgc
     3901 gggcgatgga gtggcgggtc ttggtcaggc gcgccacggc gatgtcgcgg cgcagggcct
     3961 ccttgatggc gcggaagctg gagccctcct cgtcgaggcg gcggtgcagg gtgcgcggcg
     4021 acaggtgcag gcggtcggcc acgtgctcct gggacaggtt ggccggcagc gccgcgcgga
     4081 gcaggtcgcg cacccgcacg acggtgtccc ggtcgcggcg gtagagggtg gtgatcttgc
     4141 ccggcgcgcc ttccaggaaa cgggccaggg cggcctcgtc gcggcggatc ggcagatcca
     4201 gcagggtggg gttgaagcgc gccgccagat cgtcgccgcc gaagctgaag tgggcggtgt
     4261 agatcagctg gtagtcgtcg gcgtggggcg ggcgggcgaa ggggaagcgc accgcatcca
     4321 gcgccaggcc gcgccccacc agccagcagg cgaggccgtg gagcaggcgc agcagccact
     4381 cgaaggcgaa gacccgcgcc gggtcgtccc gcgtcgggca cagggggcgg gtctcggcga
     4441 tgcgcagctc ggcgtggctg tcgaggcggt ggatgctcac cgccagatcc ggcagcacga
     4501 tgtgcaggaa gcgccgcgcc cgctccaggg cctcggccag atggggggcg ccgagcatgc
     4561 cgcggcacag gaactcgaag ctgccggtgc ccagcgggcg ctcgaaaagg ccgaagcctt
     4621 cgtcgtccag atcgcggatc acccggttgt acagcgccgc gtagcgatcc agcgggatgc
     4681 ggctggccga gtctgcaagg tcgatgtcgg tggcggccag cagcggcgcc gggtcgtggc
     4741 cccgccgctg catcccggcg accatcccgg tgacgaagcc catggccacg ctggcccgcc
     4801 tgcgctccgg ggcggcgggc gatggggcgg gggcggcgga agaggagctc atgggcatct
     4861 ccgggaccgg ctgaagggcc aatctgcagc ttggcggatt ttgcacgaat cccgacggga
     4921 acagcaatgg cgcagccgcg tccggccttc taccatgaac ctattcctaa caacagtggt
     4981 ggaggagaga agatgactga cctgagcgtg gccaagaagc tggtgaccta caagcccaag
     5041 aacaaggtgc gtttcgtgac ggccgcagcc ctgttcgacg ggcacgatgc gtccatcaac
     5101 atcatgcggc ggatcctgca atccaccggc tgcgaggtga tccacctggg gcacaaccgc
     5161 tccgtgggcg agatcgtcaa cgcggccctc caggaagacg tgcagggtat cgccatcacg
     5221 tcctaccagg gcggtcacgt ggagttcttc aagtacatga tcgacctgct gcgtgagcac
     5281 ggcggcgaga acatccgcgt cttcggcggc ggcggcggcg tgatcgtgcc ggccgagatc
     5341 aaggagctcc acgactacgg cgtcacccgc atctactcgc cggaagacgg cgccatcctc
     5401 ggcctgcagg gcatgatcaa cgacgtggtc gagcgcagcg acatggacct ctcccacgcc
     5461 ggcccccagg gcgccgacgc ggtgctggcg gccctgcgca gcggcgaccg gcgtgccctc
     5521 gcccggatca tcaccggcct cgagaacggc gcctacgcgg atgaagtgaa gaccgcgctg
     5581 atcgccgccg cgaaggagct caagacgccc accctgggca tcaccggcac cggcggcgcg
     5641 ggcaagtcct ccctcaccga cgagctggtg cggcgcttcc gcctggacca gcacgaccgc
     5701 ctcaagctgg ccatcgtgtc catcgacccg tcgcgcaagc gtaccggcgg cgcgctgctg
     5761 ggtgaccgca tccgcatgaa cgccatcgag cacccgaaca tcttcatgcg ctccctggcc
     5821 acccgcgata ccggcaagga agtctccgtg gccctgcccg aagtcatcgc cgccaccaag
     5881 ctggcgggct tcgatctggt catcgtcgag acctccggca tcggccaggg cgacgcggcc
     5941 atcgtgccct tcgtggatgt gtccctgtac gtgatgaccc ccgagttcgg cgccgccagc
     6001 cagctcgaga agatcgacat gctcgacttc gccgacttcg tggccatcaa caaattcgac
     6061 cgcaagggcg ccgaggatgc cctgcgcgac gtgcgcaagc aggtccagcg caaccgggag
     6121 ctgttcacca cccaggtgga gcagatgccc gtgttcggca ccatggccgc ccgcttcaac
     6181 gacgacggtg tcaccgccct gtaccaggcc ctcgccccgg ccctcgtcgc caagggcctc
     6241 aagctcggca agcccggcct gcccctggtg agcgtgcgcg cctcgtcccg cggccgcgcc
     6301 atcgtccccg ccgagcgtgc ccgctacctg gccgagatcg ccgacgccgt gcgcggctac
     6361 cacaagcaca ccctggagca gtcccgcatc gcccgcgagc gccaggccct gcgcatctcc
     6421 aagcgcctct tcgaaggctg ccagaaggac gccggcagct ttgacgagct gatcgactgg
     6481 aaggacggcg agctgacccc gtcctcccgc aagctcctcg acatgtggcc caagaccgtc
     6541 gagctgtacg cggccgacga gtacgtggtg aagatccgcg acaaggagat ccgtaccgcc
     6601 ctcacctccg agtccctgtc cggcagcaag atccgcaagg tggccctgcc cagcttcgag
     6661 gacgacggcg agtccctgcg cttcctgatg aaggagaacg tccccggctc cttcccctac
     6721 accgccggcg tgttcgcctt caagcgcgaa ggtgaagacc cgacccgcat gttcgccggc
     6781 gagggcgatg ccttccgcac caaccggcgc ttcaagaagg tctcggaagg catgcccgcg
     6841 caccgcctct ccaccgcctt cgactcggtg acgctctacg gctgcgaccc ggacccgcgt
     6901 ccggacatct acggcaaggt cggtaactcc ggcgtgtcta tcgccacgct ggacgacatg
     6961 aaggtgctct acgacggctt cgacctggtc aacccgacca cctcggtgtc catgaccatc
     7021 aacggcccgg cgcccatcat cctggccatg ttcttcaaca ccgcactggc ccagcaactg
     7081 gagaagttca aggcccagaa cggccgcgaa cccaccgacg acgaagccca gaagatccgc
     7141 gaatgggtcc tcgccaccgt gcgcggcacc gtccaggccg acatcctcaa ggaagaccag
     7201 ggccagaaca cctgcatctt cagcaccgag ttcgccctca agatgatggg cgacatccag
     7261 gagttcttcg tccataacaa ggtgcagaac ttctactcgg tgtccatctc cggctaccac
     7321 atcgccgaag ccggcgcgaa ccccatcagc cagctcgcct tcacgctgtc caacggcttc
     7381 acctacgtgg aaagctacct ggcccgcggc atgcacatcg acgacttcgc gcccaacctc
     7441 tccttcttct tcagcaacgg catggacccg gaatactccg tcatcggccg tgtcgcccgc
     7501 cgcatctggg cggtggccat gaagaacaag tacggcgcca acgaacgcag ccagaagctc
     7561 aagtaccaca tccagacctc gggccgctcc ctgcacgccc aggagatgga cttcaacgac
     7621 atccgcacca ccctgcaggc gctgatcgcc atctacgaca actgcaactc cctgcacacc
     7681 aacgcctacg acgaggccat caccacgccc accgaggaat ccgtgcgccg cgccatggcc
     7741 atccagctca tcatcaaccg ggagtggggc ctcgccaaga acgagaaccc caaccagggc
     7801 gccttcatca tcgaggagct caccgacctg gtggaagagg ccgtgctcaa ggagttcgag
     7861 gccatcagcg cccgcggcgg cgtgctgggc gccatggaaa ccggcttcca gcgcggcaag
     7921 atccaggagg agtcgctgta ctacgagcac cagaagcacg acggctccta ccccatcatc
     7981 ggcgtcaaca ccttcctcaa ccccaagggc atggcccagc aggaaatcga gctggcccgt
     8041 tcctccgagg acgagaagca gagccagctc cagcgcctcg ccgccttcca ggaccgcaat
     8101 tccgacgaag cgccccagtg gctggccaag ctcaagcaga ccgtgatcga gaacggcaac
     8161 gtcttcgccg tgctggtgga tgcggtgaag tactgctccc tcggccagat caccaccgcc
     8221 ctgtacgacg tgggcgggca gtatcggcgc agcatgtaag actcgcaccg catcacaaga
     8281 accctcggta agaggggaac agcagaacgg cgccttcggg cgccgttcgt ttaagtgcac
     8341 cagcaccttg tggcggcggc gagaggaccg gttactcgtg gagatgcatg gctcatgcta
     8401 ggatgaccat gattgatgac cataggggac aggccatgca caccaccatc ggtgcgggtg
     8461 acttcaaggc gcggtgcctg aaattgctgg acgacgtggc ccgttccggc gaggctctgg
     8521 tgattacgaa acacggcaag ccggttgcga agctggtgcc gatgccgccc gccgggagcc
     8581 tgttcggtgc gctggccggc agtgtgcggc aggagggcga catcgtggcg cccgtcgatg
     8641 acgactggga tgcctgtcgt tgagccccct gctgctggac acccacgtcc tggtctggct
     8701 gctcgccggc agcgatcgtc tcggcctccg tgcgcgggcc gccattgatg cggcagccgc
     8761 ggtcgaaggg gtgttcgtgt cggccatcac cccctgggaa attgccatgc tcgtcgccaa
     8821 gggacgtctg gtcctggagc gggatgtggg cgactgggtg caggcggctc tggccttgcc
     8881 gggcatcagg ctcgaagcgc tctcgccggc cgtggcggtg gcgagcacgc ggctgcccgg
     8941 cgcgctgcac gccgatccga cagaccggat cattgtcgcc actgcccgcc acctgggcgc
     9001 agcgctggtg acggccgacg gcttgctgct cgactacgcc tcgagcggaa atctgaaggt
     9061 gttgccggcg gggcagtagc acgtcactct atgccgccgg tcctccgcga agacctgctc
     9121 tgtgcgccgt gggacgcgct cagcgccgtc cgagcgggat cgtcccgagg cgcgggccac
     9181 ggatccgctc ccctcactgc cccgccagcg catccaccat cgcccgcagc gtgcggatct
     9241 gcac
//
LOCUS       DAOQMM010000127         9076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3780_length_9076_cov_52.960537, whole genome shotgun sequence.
ACCESSION   DAOQMM010000127 DAOQMM010000000
VERSION     DAOQMM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3780_length_9076_cov_52.960537"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="PLH85_15445"
     CDS             complement(<1..1915)
                     /locus_tag="PLH85_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNB66013.1"
                     /translation="MTARSPAPDSIPSPGNPLPELRQAIVALTRAPEAECMARLFGDA
                     RMAPEALARVCARARQLAEGVRAGRVRAGGVDALMHEFALGSAEGVALMCLAEALLRV
                     PDAAGRDALIRDKLHARDWSAHVGHSPSLFVNAAAWSLALTGRLLSADESARLPGTLA
                     RMATRGGEAVVREAMEFAMRRVGRQFVMGETIEEALERVASGAAADYRYSFDMLGEAA
                     LTADDATRYAAAYAHAIHAIGGADHWRGVRAGNGISIKLSALHPRYTWSQRDRVMAEL
                     LPRVHHLCVLARGHDIGLTIDAEESDRLELSLDLFAALATDPDLRDWAGLGMVVQAYQ
                     KRAPAVIDWLIALGRRSGRRIMTRLVKGAYWDGEIKRAQQDGLADYPVYTRKVHTDTA
                     YLACARRMLAAPDAIYPAFATHNAHTVAAVLEMAGPFHDGAYEFQCLHGMGETLYRQL
                     VAGADPAMRRPVRVYAPVGSHETLLAYLVRRLLENGANTSFVHRVVDDSVPLDMLVAD
                     PVERAVAQGGAPHPAIPLPGALFVERANSRGFDLAAAPVRRAFLAALEKSRASTFTAV
                     PRVVGEAAAGDPSPSIQIRNPARRSDFVGTAACADAWQIEAALAAAAGAAAGWAATTV
                     TRKRSSMLSSNTAP"
     gene            complement(2027..3028)
                     /locus_tag="PLH85_15450"
     CDS             complement(2027..3028)
                     /locus_tag="PLH85_15450"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNB66014.1"
                     /translation="MRRVTLTQFLIEQQRKGLMGADLRLLMEVVARAVKAIAVNVSKG
                     ALAGVLGEAGSDNVQGEAQKKLDVIANDILIQANEWGGHLAAMASEEVEEIRPISGEY
                     PKGAYLLLFDPLDGSSNIDVNISVGTIFSVLRCPEGVSAPTEADFMQPGTAQVAAGYS
                     VYGPSTQLILTVGDGVHGFTLDREMGSFIYTHPNMTIPADTKEYAINASNARFWEAPV
                     QRYIGELQAGKEGPRGKDFNMRWVASMVADVHRILTRGGIFMYPMDSKMKDKGGKLRL
                     MYEANPMAMIVEAAGGAATTGRERILDIQPSRLHQRVPVVLGSKNEVERVTAYHLEG"
     gene            3173..4435
                     /locus_tag="PLH85_15455"
     CDS             3173..4435
                     /locus_tag="PLH85_15455"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNB66015.1"
                     /translation="MKVLVIGAGLEGVTSAWYLAQAGCEVTVVERRPGPGLETSFANG
                     GQISVSHPEPWANPRAPGTILRWIGRDDAPLLFRPSLDGRQWRWGLAFLRECLPARTA
                     RNTAAIASLACYSRDQLRALRDATGLEYAAQTQGILHLFTTSRELVHARDHERILSRL
                     GIRANLLTSAQAISLEPALAPLADRLKGALHGVDDESGDAHLFCRALAERAAAAGVTF
                     LYDSAVYSMETLAGRVAGVRVLDSARRAGVLTADAFVVAAGSYSPALLARVGEALNIY
                     PVKGYSVTAQIVDPERAPHLSLTDESRRVVCSRFGDRLRVAGTAELNGFDLSPNPARS
                     NALVRWLEEHFPGVTHSRKAEHWCGLRPATPSNVPIIGRGRYDNLFLNAGHGTLGWTL
                     ACGSARALADIITGRKPEPEFPFRGLAR"
     gene            complement(4437..5312)
                     /locus_tag="PLH85_15460"
     CDS             complement(4437..5312)
                     /locus_tag="PLH85_15460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNB66016.1"
                     /translation="MTFVRVGIDEIEIGQPLRWPLYDDKKQPLLARGAVVENAERKAA
                     LFGQGVFRKTVEDERPTGEGGERPAEDLRGLDEIRMDIGDTLQLQAQSETSQSRHYVK
                     LIGYLKGKSVVVTTPTQDGKVLLMREGQAFVVRMFSGKSVYAFGATIFKVANVPYPHL
                     HLTYPSQVRGLVVRRGARARVNLIAAVSGADGRSHAGTVVDLSIGGAMLVAKSPIGLK
                     DDRIKVKFRVVVSDVEQYLTLDAVLRTVHATAATEGEPSMVNHGLQFDSLPHAEQVVL
                     SAYVYRVLFEESTEG"
     gene            complement(5402..5902)
                     /locus_tag="PLH85_15465"
     CDS             complement(5402..5902)
                     /locus_tag="PLH85_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB66017.1"
                     /translation="MAAMQWTDKLALGLGPMDDTHKEFVDHYNRLLQVTGEALLTELD
                     AFIAHTVAHFDQENRWMEQVDFPACHKAEHDRVLAVVADVRKRMERGDEALGRQLIQE
                     LPIWFDNHVATMDAALAAYLESIGYDTATGEIHRPPQVDACGNPVEAGACCTPAPVSP
                     VEGSPA"
     gene            complement(5988..6425)
                     /locus_tag="PLH85_15470"
     CDS             complement(5988..6425)
                     /locus_tag="PLH85_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNB66018.1"
                     /translation="MSEVSTKPYLIRAIFQWCVDQGLTPYLSVHVDGRTQVPRAFVQD
                     GQIVLNVSMDATQNLVMGNELIAFQARFGGVAHHIAVPVDNVSAIYARENGHGMAFEV
                     NPSDPAEASEPDATASESPPATADTPPDDGPKGGARGRLKLVK"
     gene            complement(6479..7075)
                     /locus_tag="PLH85_15475"
     CDS             complement(6479..7075)
                     /locus_tag="PLH85_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNB66019.1"
                     /translation="MMNLYSGTTDPFSHRCRIVLFEKGMDFQVIDVDLFNKPEDIAVI
                     NPYNRVPVLVDRDLILYESNIINEYIDERFPHPQLMPPDPIMRARARQLLHTFELELF
                     SHVEALEKNAKEADKERAHIRDQLTQLAPIFTKQKFLLGEEFSMLDVAIAPLLWRLDH
                     YGIELPRTAAPLMKYAERIFSRQGFIDAMTPSEKVMRR"
     gene            complement(7183..7950)
                     /locus_tag="PLH85_15480"
     CDS             complement(7183..7950)
                     /locus_tag="PLH85_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNB66020.1"
                     /translation="MKTRGLSLVKKLFATALFAPLLAVASGPELHLDKAPVSSDPAAL
                     QNGAKLFVNYCLNCHGASYLRYNRLKEIGLSDQMIKDNLMFTADKIGEPMRIAMQRDE
                     AKAWFGAAPPDLTLIARSRASEFGSGADWLYTYLRSFYRDPGRPTGWNNTVFENVGMP
                     HVLYELQGDQVAKFEEHDDGHGNMTKVVHIEPGKPGKMSKDEYDKATADLVSFLVWMG
                     EPMAGTRKTIGTFVLAFLGVLFVLSYLLKKNYWKDVH"
     gene            complement(7947..>9076)
                     /locus_tag="PLH85_15485"
     CDS             complement(7947..>9076)
                     /locus_tag="PLH85_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNB66021.1"
                     /translation="VLVIQIVTGIFLVMHYKPDASLNASGVPVAFASVEYIMRDVPGG
                     WIIRYMHSTGASAFFIVVYLHMFRGLLYGSYRKPRELIWIFGTLIFLALMAEAFMGYL
                     LPWGQMSFWGAQVIVNLFSAIPVIGPDLSVVIRGDFVVSDATLNRFFSFHVIAVPLIL
                     IGLVAAHIVALHEVGSNNPDGVEIKKLKGADGHPLDGIPFHPYYTVKDIVGVVGFLIV
                     FSIIVFFAPEGGGYFLEFNNFIPADPLKTPPHIAPVWYFTPFYSILRAVTYPLFGIDA
                     KFWGVVAMGAAVIIIAFLPWLDRSPVKSIRYKGGLFKAILAVFIVAFFILGYLGVLPP
                     TPGRTLVSQICSVIYFGFFLLMPWYSKIDKTKPEPERVTWK"
BASE COUNT         1666 a         2912 c         2963 g         1535 t
ORIGIN      
        1 tcggcgcagt gttcgacgac aacatggagg accgcttgcg cgtcaccgtc gtggccgccc
       61 agccggcagc cgcgcccgcc gcggcggcca gcgcggcttc gatctgccac gcatcggcac
      121 aggctgcggt gccgacgaag tcgctgcggc gggcaggatt ccggatctgg atggacgggg
      181 acggatcgcc cgcagcggcc tcaccgacca cccgcggcac ggccgtgaag gtgcttgcgc
      241 ggctcttctc cagcgctgcg agaaaggccc gccggaccgg cgcggccgcc agatcgaagc
      301 cgcggctgtt ggcacgctcg acgaacaggg cgcccggtag cgggatggcc ggatggggcg
      361 ccccgccctg ggccaccgcc cgttcgaccg ggtcggcgac cagcatgtcg agggggaccg
      421 agtcatccac gacacgatgc acgaagctgg tgttggcgcc gttctccagg aggcgccgca
      481 ccagataggc cagcagggtt tcgtggctgc cgaccggcgc gtacacccgc acgggacggc
      541 gcatggcggg atccgcaccg gccaccagct ggcgatagag ggtttcgccc atgccatgca
      601 gacactggaa ctcgtaggcg ccgtcgtgga acggacccgc catctcgagg accgccgcga
      661 ccgtatgggc gttgtgggtg gcgaaggccg ggtagatcgc gtccggcgct gccagcatgc
      721 ggcgggcaca ggccaggtag gcggtgtcgg tgtggacctt gcgggtgtag accgggtaat
      781 cggcgaggcc gtcctgctgg gcgcgcttga tttcgccatc ccagtaggcg cccttgacca
      841 ggcgggtcat gatgcgccgc ccgctgcgcc gcccgagggc gatgagccag tcgatgactg
      901 ccggcgcgcg cttctggtag gcctgcacga ccatgcccag ccccgcccag tcgcgcagat
      961 ccgggtcggt ggccagcgca gcaaagagat cgagggacag ctcgaggcgg tcggactctt
     1021 cggcgtcaat ggtcaggccg atatcgtgcc cccgcgccag gacgcacagg tgatgcaccc
     1081 gtggcaggag ttcggccatc acccggtccc gctgggacca ggtgtagcgt ggatgcaggg
     1141 ccgacagttt gatggagatg ccattgccgg cgcgcacgcc gcgccagtga tcggccccgc
     1201 cgatggcgtg gatggcatgg gcgtaggcgg cggcgtagcg ggtcgcgtcg tcggcggtga
     1261 gcgctgcctc accgagcatg tcgaaggagt agcgatagtc cgccgcggcg ccgctggcga
     1321 cgcgctccag cgcctcctcg atggtttcac ccatcacgaa ctgccgacca acccggcgca
     1381 tggcgaattc catggcttcc cggaccaccg cctcgccgcc gcgggtggcc atccgggcca
     1441 acgtgccggg caggcgcgcg gactcatcgg cggacagcag ccggccggtc agcgccagac
     1501 tccaggcggc ggcattgacg aagagactcg ggctgtggcc gacgtgcgcg gaccaatcgc
     1561 gggcgtgcag cttgtcgcga atcagcgcgt cccgccccgc cgcatcgggt accctcagca
     1621 aagcctctgc gaggcacatc agcgcaaccc cctcggcgct gccaagggcg aattcatgca
     1681 tcagggcatc gacacctcct gcccggacac gtcctgcccg aacgccctcg gccagttgcc
     1741 gcgcccgggc gcagacccgc gccagcgcct ccggcgccat ccgggcatca ccaaagagac
     1801 gcgccatgca ttcggcttcg ggtgctcggg tgagcgcgac aatcgcctgg cgcagctcgg
     1861 gcaagggatt gccgggcgac gggatggaat cgggggcggg cgaacgggca gtcatggcat
     1921 caccggtacg gctcaaagaa aagggggcgc tccgttcgac cggaaaaacg ccccgcgatt
     1981 ccaggcctgg ccacacaccg cagggtggtg gcctgccgac gcatcgtcag ccttccaggt
     2041 ggtaggcggt gacacgctcc acttcattct tcgaccccag cacaaccggg actcgctggt
     2101 gcagccggga aggctggatg tcgaggatgc gttcccgccc ggtggtggcg gcgccgcccg
     2161 cagcttcgac gatcatcgcc atgggattgg cttcgtacat caggcgcagc ttgccgccct
     2221 tgtccttcat cttgctgtcc atcgggtaca tgaaaatgcc cccgcgggtc aggatgcgat
     2281 gcacgtctgc gaccatcgag gccacccagc gcatgttgaa gtccttgcca cgggggcctt
     2341 ccttgcccgc ctgaagctcg ccgatgtagc gctgcaccgg cgcttcccag aagcgcgcat
     2401 tggacgcgtt gatggcgtat tccttggtgt cggcgggaat cgtcatgttc ggatgggtgt
     2461 agatgaagct gcccatctcg cgatcgaggg tgaacccatg cacgccatca ccgacggtga
     2521 gaatgagctg cgtggagggg ccatacaccg agtagcccgc cgccacctgg gccgtgccgg
     2581 gctgcatgaa atcggcttcc gtcggcgcgg aaacgccttc tgggcagcgc aacaccgaga
     2641 agatggtccc gacggagatg ttcacatcga tgttggacga accgtcgagg gggtcgaaca
     2701 gcagcaggta ggcacccttc gggtattcac cggaaatcgg ccggatctct tcgacctcct
     2761 cggatgccat ggccgccagg tggccgcccc actcgttggc ctgaatcagg atgtcattgg
     2821 cgatcacatc cagcttcttc tgggcctcgc cctggacgtt gtcactgccc gcctccccga
     2881 gcacaccggc cagcgcacct ttggagacat tgacggcgat ggccttgacc gcccgcgcca
     2941 cgacttccat gagcagccgc aggtcggcgc ccatcaggcc cttgcgctgt tgttcgatca
     3001 gaaactgggt gagcgtgaca cgacgcatgg cgtatttctc ctgagcaatt ccggtaaagt
     3061 ccggattatc gcccggaatg cgctgctgcg tcgtttatga ttacagggtt cgaagcatcg
     3121 acaccctctt tggcggtggc gccagccgcc ccggaaatat tgtttcagca tcatgaaagt
     3181 tctcgtcatc ggcgccggcc tggaaggcgt caccagcgcc tggtatctgg cccaggcagg
     3241 ttgcgaagtc accgtcgtgg aacgtcgtcc cggccccggc ctggaaacca gctttgccaa
     3301 cggcggccag atctccgtga gccatccgga accctgggcc aatccgcggg caccggggac
     3361 gatcctgcgc tggatcggtc gtgacgacgc ccccctgctg ttccggccca gcctcgatgg
     3421 tcgccagtgg cgctggggac tggcctttct acgggaatgc ctgcccgcgc gcacggcccg
     3481 caatacggcc gcgatcgcca gcctcgcctg ctacagccgt gaccagctcc gcgccctgcg
     3541 tgacgccacc gggctcgaat atgcggcgca gacccagggc atcctgcatc tgttcaccac
     3601 atcgcgggag cttgtccatg cccgtgacca cgagcgcatc ctgtcgcgcc ttggcattcg
     3661 ggcaaacctc ctgacgtccg cccaggcgat cagcctggag ccggccctcg caccgctggc
     3721 cgatcgactg aaaggcgccc tgcacggagt cgacgacgaa tccggtgatg cgcacctgtt
     3781 ctgccgcgcc ctggccgagc gcgccgccgc cgcaggggtg accttcctgt acgacagcgc
     3841 cgtgtattcc atggagacgc tcgcgggtcg cgtggccggc gtgcgtgtcc tcgactcggc
     3901 ccgtcgcgcc ggcgtgctga ccgcggacgc cttcgtcgtc gcggcgggca gctacagtcc
     3961 ggccctcctg gcgcgcgtgg gggaggccct caacatctac ccggtgaagg gctactcggt
     4021 caccgcgcag atcgtggatc ccgaacgggc gccgcacctg agcctcaccg acgagtcccg
     4081 ccgtgtggtg tgctcacgct tcggcgatcg gctgcgggtg gccggcaccg cggagctgaa
     4141 cggcttcgac ctgagcccca acccggcccg cagcaatgcc ctggtgcgct ggctggaaga
     4201 acattttccc ggtgtcaccc atagccgcaa ggcggaacac tggtgcgggc tcaggccggc
     4261 aacacccagc aatgtgccca tcatcggacg gggccgttac gacaacctgt tcctgaatgc
     4321 cgggcacggc accctgggct ggaccctggc ctgcggatcg gcgcgggcgc tcgccgacat
     4381 catcacgggc cgcaagcccg aaccggagtt tccgttccgg ggcctggcgc gctgactcag
     4441 ccttcggtgg actcttcaaa cagcacgcgg taaacgtagg cggacaggac gacctgttcg
     4501 gcgtggggca ggctgtcgaa ctgaagaccg tgattgacca tggacggctc accttcggtc
     4561 gccgccgtgg cgtgaaccgt acgcagcact gcatcgaggg tcaggtactg ctccacatca
     4621 ctgaccacca cgcggaactt caccttgatc cggtcatcct tgagcccaat cggggacttg
     4681 gccaccagca tggcaccacc gatgctcaga tcgacgaccg tgcccgcatg ggaacgcccg
     4741 tcagcaccgg ataccgccgc gatgagattg acccgcgccc gggcccctcg acgcaccacg
     4801 agcccccgga cctggcttgg ataggtgagg tggagatggg gatagggtac gttcgcgacc
     4861 ttgaagatgg tcgcgccaaa ggcataaacg ctcttgcccg agaacatgcg caccacgaag
     4921 gcctgtccct cgcgcatcag cagcaccttg ccgtcttgag tcggtgtggt gacgacgacg
     4981 ctcttgccct tgaggtagcc aatgagcttg acgtaatggc ggctctggga tgtctcgctc
     5041 tgggcctgca actgcagggt gtcgccgata tccatgcgga tctcgtcaag cccgcgtaga
     5101 tcctcggccg gacgctcccc gccctcgccc gtcggacgtt catcctcgac cgtcttgcga
     5161 aacacgccct gtccgaacag ggccgccttt cgctctgcat tctcgaccac cgccccgcgg
     5221 gcaagcaggg gctgcttctt gtcgtcgtag aggggccagc gcaggggctg accgatttca
     5281 atctcgtcga ttccgacgcg gacgaacgtc atgactgact cgcttgctga acacaacaac
     5341 gagacctatc gtcaccgacc ggaaaaacct tagcgaagcg gaatacacgc cgcgccgtcg
     5401 atcaggcggg cgagccttcc accggactga caggggccgg cgtacaacac gcgcccgcct
     5461 cgaccggatt gccacatgca tccacctggg gcggacggtg gatctcgccc gtggcagtgt
     5521 cgtatccgat ggattccaga taagccgcca gcgcagcgtc catcgtcgcc acatgattgt
     5581 cgaaccagat cgggagttcc tggatcagct ggcgtccgag cgcctcatca ccccgctcca
     5641 tccgcttgcg cacatccgcc acgacggcaa gcacccggtc gtgttcggcc ttgtgacacg
     5701 cggggaaatc gacctgctcc atccagcggt tttcctggtc gaagtgggcg accgtatgcg
     5761 caatgaaggc gtccagttcc gtcagcaagg cctcgccggt cacctggagg aggcggttgt
     5821 aatgatcgac gaactccttg tgggtatcgt ccatcggccc cagacccaga gccagtttgt
     5881 cagtccattg catggcggcc atgtgatgaa ttccgtagcg aaagaggaca gcttagcgcc
     5941 cgccgtacag acccaccctg aggcaagtca agcggtgtcg cgccggctta tttgacgagc
     6001 ttgaggcgcc cccgggcacc gcctttcggc ccatcgtcgg gcggcgtatc ggcagttgca
     6061 ggcggcgact cggaagccgt ggcatcgggc tcggacgctt cagccggatc ggacggattg
     6121 acctcgaacg ccatcccgtg accgttctcg cgggcataga tcgccgacac attgtctaca
     6181 gggaccgcga tgtggtgggc gacgcctccg aatcgggcct ggaaggcaat cagttcattg
     6241 cccatcacca gattctgggt cgcatccatg ctcacattga ggacgatctg tccgtcctgg
     6301 acgaacgcac gcggcacctg cgtccgccca tcgacatgta cggacaggta gggcgtcagc
     6361 ccctgatcga cgcaccactg gaagatggcg cggatcagat aaggcttggt ggaaacttca
     6421 ctcatgggac tggatcctgc aaaggcgcac gcccggagga cgcgcgccag acccgcgctc
     6481 agcggcgcat gaccttttcc gacggggtca tcgcgtcaat gaacccctga cggctgaaga
     6541 tgcgctcggc gtatttcatc agcggcgcag cggtacgcgg cagctcgatg ccgtagtggt
     6601 caagacgcca cagcagcggc gcgatggcca cgtccagcat ggagaattcc tcgccgagga
     6661 ggaatttctg cttcgtgaag atcggcgcca actgggtcag ctggtcgcgg atatgggccc
     6721 gctccttgtc cgcctccttg gcgttcttct cgagggcctc cacatgggag aacagctcga
     6781 gttcgaaggt atgcaacagc tggcgggccc gcgcacgcat gatgggatcg ggcggcatca
     6841 gctggggatg cgggaaacgc tcgtcgatgt actcgttgat gatgttcgat tcgtacagaa
     6901 tcaggtcacg atcaaccagc acgggaaccc ggttgtacgg attgatgacg gcgatgtctt
     6961 ccggcttgtt gaagagatcg acgtcaatca cctggaaatc catgcccttt tcgaagagca
     7021 cgatgcgaca gcggtgactg aacggatccg tcgtcccgga atacagattc atcatcgttg
     7081 tgttacccca cggatactca gaatcacgca ccacgcgacg ggcgcgtggt gcgtcttgct
     7141 caagaacagc aagagactgc atcggctgaa agcagctgcc gattagtgaa cgtctttcca
     7201 gtaattcttc ttgagaaggt aggaaagcac gaagaggacg cccaggaagg cgagaacgaa
     7261 ggtgccgatg gtcttgcggg tgcccgccat gggctcaccc atccacacga ggaaggagac
     7321 gagatcggcg gtggccttgt cgtattcatc cttgctcatc ttgcccggct tgcccggttc
     7381 gatgtgcacg accttggtca tgttgccatg accgtcatcg tgctcctcga acttggccac
     7441 ctgatcgccc tgcagttcat acaggacatg aggcatgcca acgttttcga acacggtgtt
     7501 gttccagccg gtcggccggc ccggatcgcg gtagaagctg cgcagatagg tgtagagcca
     7561 gtctgcacca ctgccgaact cggaggcgcg ggaacgggcg atcagggtca gatccggagg
     7621 cgcggcaccg aaccaggcct tggcttcatc acgctgcatg gcgatgcgca tcggctcacc
     7681 gatcttgtcg gcggtgaaca tcaggttgtc cttgatcatc tgatcggaca gaccgatttc
     7741 cttcagccgg ttgtagcgca gatagctcgc accgtgacag ttcaggcagt aattgacgaa
     7801 gagcttggcg ccgttctgga gcgcagccgg gtcagaagac accggggcct tgtccaggtg
     7861 caactcgggg ccgctcgcga ccgccagcag cggcgcgaac agggcagtgg cgaacagctt
     7921 tttcacgaga ctgagtccgc gggttttcat ttccaggtca ccctttccgg ttcgggtttg
     7981 gtcttgtcga tcttggagta ccacggcatc agcaggaaga aaccgaagta gatcaccgag
     8041 cagatttgcg acaccagggt acggcccggg gtcgggggca ggacgccaag gtagccgagg
     8101 atgaagaagg ccacgatgaa gaccgcgagg atggccttga agaggccgcc cttgtagcgg
     8161 atggacttca ccgggctgcg gtcaagccag ggcaggaagg cgatgatgat caccgcggca
     8221 cccatggcca ctacgcccca gaacttcgcg tcgataccga acagcggata ggtcaccgcg
     8281 cggaggatcg agtagaacgg ggtgaagtac cacaccggcg caatgtgcgg cggcgtcttc
     8341 agcggatcgg cggggatgaa gttgttgaac tccaggaagt agccgccgcc ttcaggcgcg
     8401 aagaacacga tgatggaaaa gacgatcagg aaaccgacca cgcccacgat gtccttgacg
     8461 gtgtagtagg ggtggaaggg gatgccgtcc agaggatggc cgtccgcgcc cttgagcttc
     8521 ttgatctcga caccgtccgg gttgttggag cccacttcgt gcagggcaac gatgtgcgcc
     8581 gccaccaggc cgatgaggat cagcggcacg gcgatgacgt ggaaggagaa gaagcggttc
     8641 agggtggcat cggacaccac gaagtcgccg cggatgacca cggacagatc cgggccgatc
     8701 acgggaatcg cggagaacag gttcacgatc acctgggcgc cccagaagga catctgcccc
     8761 catggcagca gatagcccat gaaggcttca gccatcagcg ccaggaagat cagggtgccg
     8821 aagatccaga tcagttcgcg gggcttgcgg taggaaccgt agagcaggcc gcggaacatg
     8881 tggaggtaga ccacgatgaa gaacgcggag gcgccggtgg agtgcatgta gcggatgatc
     8941 cagccgccgg gcacatcgcg catgatgtac tcgacggagg cgaaggccac cggcacgccg
     9001 gaagcgttca gggaggcgtc cggcttgtag tgcatcacca ggaagatgcc ggtgacgatc
     9061 tggatcacca gcacca
//
LOCUS       DAOQMM010000128         9039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3799_length_9039_cov_43.690227, whole genome shotgun sequence.
ACCESSION   DAOQMM010000128 DAOQMM010000000
VERSION     DAOQMM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_9039_cov_43.690227"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..827)
                     /gene="urtD"
                     /locus_tag="PLH85_15490"
     CDS             complement(6..827)
                     /gene="urtD"
                     /locus_tag="PLH85_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNB66022.1"
                     /translation="MSAIPANQDGGNASSLLGHVATGDLDLSHGVILYLEDITVSFDG
                     FKALNSLNLSIDAGELRCIIGPNGAGKTTMMDVITGKTRPDGGKAYFGQTIDLTRLTE
                     PQIAHMGIGRKFQKPTVFEQHSVFENLELAMKTDKRVQRSLLAWLTGDDRDRIADTLR
                     QIRLDGVADRPAGLLSHGQKQWLEIGMLLIQDPRLLLLDEPVAGMTDDETMRTAELFV
                     SLAGKHSLVVVEHDMAFVERLGGKVTVLHEGSVLAEGDLATVQNDQRVIEVYLGR"
     gene            complement(824..1939)
                     /gene="urtC"
                     /locus_tag="PLH85_15495"
     CDS             complement(824..1939)
                     /gene="urtC"
                     /locus_tag="PLH85_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNB66023.1"
                     /translation="MHKPLTLRILAALDAKGWLALGLFLAVTLVLVPVLHLTVSPDSP
                     FHVSAYAITLIGKIMCYALVAVAMDLIWGFGGILSLGHGLFFALGGYAFGMYLMRQIG
                     RDGSYQSDLPDFMVFLDWKELPWYWSGSDSFLWCALLVVGVPALVAFVFGYFAFRSRI
                     KGVYFSIITQALTYAAMLLFFRNETGFGGNNGFTDFKRVLGYSITSPETRLVLFGLTA
                     FTLAATLVLARWLVTSKYGRVLAAIRDAESRVMFLGYNPLWYKLFVWVLSAALCGIAG
                     ALYVPQVGIINPGEMSTANSIEIAIWVAVGGRGTLIGPVIGAFTVNLAKSWFTVSFPE
                     YWLFFLGLLFILVTLFLPQGLVGLWKKIVARREGARA"
     gene            complement(1950..3530)
                     /gene="urtB"
                     /locus_tag="PLH85_15500"
     CDS             complement(1950..3530)
                     /gene="urtB"
                     /locus_tag="PLH85_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNB66024.1"
                     /translation="MIKRLLFLLLCACSLAAHAALDAAQVRKLADDDSTEKVAAIRAL
                     VQSADPDALRVLQAMADDSLYVAGDKVVIVADDKAFDAASGAAIPMPDSPDSVTVNNR
                     IRGELAGALAALRLFDGDPAVRKDSAQKLQTGATPELAPLLARALDKESDAGIKALLA
                     TAHAQATLGSPDPAVRLAAVRALADSTSPTIRSQLQALTTVEGEADEKVRYEAVTAVK
                     AIESRLAFAENLGRVFSGLSLGSILLLIALGLAITYGVMGVINMAHGELLMVGAYSAY
                     AMQSVFRAYFPDAIDWYLPAAVPVAFLAAALVGVVMERTVIRWLYGRTLETLLATWGI
                     SLVLIQAARVLFGAQNVEVSNPSWMSGGIELMPNLMLPWNRIIIIGFALFVLTLVWAL
                     MNRTRLGMFVRAVTQNRAMAGCVGVPTSRIDTLAFALGAGIAGLGGVALSQISNVGPD
                     MGQGYIVDSFMVVVLGGVGQLAGAVWAALGLGVVSKLLEGWAGAVIAKIVVLVFIIVF
                     IQKRPQGLFALKGRFVDN"
     gene            complement(3662..4105)
                     /gene="cynS"
                     /locus_tag="PLH85_15505"
     CDS             complement(3662..4105)
                     /gene="cynS"
                     /locus_tag="PLH85_15505"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HNB66025.1"
                     /translation="MKREDVTDLILVRKLKKQLSWARLAEIVGQSKEWTTAALLGQMT
                     LSAAQAQAVGAALDLPDEAVEQLQIVPYKGSLPTAVPTDPLIYRFYELVNVYGTTFKA
                     LIHEEFGDGIMSAIDFHMDLTREPDPKGDRVKIVMSGKFLPYKTY"
     gene            complement(4225..5496)
                     /gene="urtA"
                     /locus_tag="PLH85_15510"
     CDS             complement(4225..5496)
                     /gene="urtA"
                     /locus_tag="PLH85_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HNB66026.1"
                     /translation="MSNRRTFIKATALSLAMATAGFASFAAQAADTIKVGILHSLSGT
                     MAISETALKETALMTIDEINKAGGVMGKKLEPVVVDPASNWPLFAEKARQLLTKDKVA
                     VTFGCWTSVSRKSVLPVYKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYL
                     MSKDGGEAKRFVLLGTDYVYPRTTNKILRAFLHSKGVKDEDIMEDYTPFGHSDYQTII
                     AKIKKFASEGKKTAVVSTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDT
                     KPLVGHLAAWNYFMSLKNPENTKFVKMYKDWAKKQKLPNADKVVTNDPMEATYIGIHM
                     WAQAVEKAKSTDVDKVIAAMAGQTFKAPSGFVAKMDEKNHHLHKPVFIGEVKADGQFN
                     VVWKTPGPVRAAPWSPYIPGNDKKKDEPEGK"
     gene            complement(5749..6024)
                     /locus_tag="PLH85_15515"
     CDS             complement(5749..6024)
                     /locus_tag="PLH85_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNB66027.1"
                     /translation="MRDSSQIKPISDLITNAAEVLTHLAEQREPMVITQNCEAKAVLQ
                     DIASFEETQETLALQKILALGHREAVAGKLKPVADVVARLRAKRASD"
     gene            complement(6153..6794)
                     /locus_tag="PLH85_15520"
     CDS             complement(6153..6794)
                     /locus_tag="PLH85_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66028.1"
                     /translation="MKMMMTSIGQMNNETAKALYKMAGRAADGFVSMGQVAAADRNVA
                     WAKIVSNYLTTSKLPIPTAIAEQAAMHISMGEKAARMIQVTAPAADATIAQTARVTAN
                     ATASVANATVSQAVKSVARCAAPITLAVFAIETSITIHKYSTGKIDIAEAKKRTAESA
                     ATNSGGLGGAIAGAAIGTAFFPGVGTAIGGLIGGVGGAIGSSKLVKWFTRRQS"
     gene            complement(6968..7783)
                     /locus_tag="PLH85_15525"
     CDS             complement(6968..7783)
                     /locus_tag="PLH85_15525"
                     /inference="COORDINATES: protein motif:HMM:NF015914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HNB66029.1"
                     /translation="MANYITTDHDLIKIIQAAEESDLAIIADFITDRNNGRISLDTTI
                     RTQIDTFKRCATLKNNSDLLIKEVQEFGGNSIVNFFRGSGVVYREILDDVADHMKVKI
                     EKTDSAEQIELKILLAVAVKGMEQMKPEEQKSFFSKLTGGKIVGFGPGAIAALQASIL
                     AGGFSSYILATTVANAVATTLIGRGLALGVNAAFMRGLAGIAGPIGWAITAIWTAFDL
                     ASPAYRVTVPCVVQIAYMRQKAKVTEFPSCSQCGSAMTPDQKFCAECGAKSGV"
     gene            complement(7798..8412)
                     /locus_tag="PLH85_15530"
     CDS             complement(7798..8412)
                     /locus_tag="PLH85_15530"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB66030.1"
                     /translation="MKNDNDLDNCHQALYYAVQEAFVENLVKTSRVAGGVSENHLTHI
                     NKGKLAELSNLSKGTITKLTSNDPQDAKPDLETLCKLGHALNVSPAFLLMTQRDWSIL
                     IQACGTLEMLKNRDGDKERTLVDCLSKASMLQKIDDAAMAGLRFMNELTGENYSDAER
                     ERQQKGILAMTAMAQAAVKRQGSDARMFAIALGAILGDREISQT"
BASE COUNT         1672 a         2657 c         2853 g         1857 t
ORIGIN      
        1 gcatctcagc gccccagata gacttcaatc acgcgttgat cgttctggac cgtggccaga
       61 tcgccttcgg ccagcaccga gccctcgtgg agcaccgtga ccttgccgcc caggcgttcg
      121 acgaaggcca tgtcgtgctc gacgaccacc agggaatgct tgccggcgag ggatacgaag
      181 agctcggccg tgcgcatggt ctcgtcgtcg gtcatgccgg cgacgggttc gtccagcagc
      241 agcaggcgcg ggtcctggat cagcagcatg ccgatctcca gccactgctt ctggccgtgg
      301 gagagcagcc cggcgggccg gtccgccacg ccgtccaggc ggatctggcg cagggtgtcg
      361 gcgatgcggt cgcggtcgtc gccggtcagc caggcgagca ggctgcgctg gacgcgcttg
      421 tcggtcttca tggccagttc caggttctcg aacacgctgt gctgctcgaa gaccgtgggc
      481 ttctggaact tgcggccgat gcccatgtgg gcgatctgcg gttcggtgag gcgggtcagg
      541 tcgatggtct ggccgaagta ggccttgccg ccgtcgggcc gggtcttgcc ggtgatcacg
      601 tccatcatgg tggtcttgcc ggcgccgttg gggccgatga tgcagcgcag ctcgcccgcg
      661 tcgatggaca ggttgaggct gttgagggcc ttgaagccgt cgaagctgac ggtgatgtcc
      721 tccaggtaga ggatgacgcc gtggctcagg tccaggtcgc cggtggcgac gtgacccagc
      781 aggctgctgg cgttgccgcc gtcctggttg gcggggatgg cactcatgca cgggctcctt
      841 cgcggcgggc gacgatcttc ttccacaggc ccacgaggcc ctggggcagg aagagggtga
      901 cgaggatgaa cagcaggccg aggaagaaca gccagtattc cgggaagctc acggtgaacc
      961 agctcttggc gaggttcacc gtaaaggcgc cgatgaccgg cccgatgagg gtgccgcggc
     1021 cgcccaccgc cacccagatg gcgatctcga tggagttggc ggtggacatc tcgcccgggt
     1081 tgatgatgcc cacctggggc acgtagagcg cgccggcgat gccgcacagg gcggccgaca
     1141 gcacccacac gaagagcttg taccagagcg ggttgtagcc caggaacatg acccgcgatt
     1201 cggcgtcgcg gatcgccgcc agcacgcggc cgtacttgga cgtcaccagc cagcgcgcca
     1261 gcaccagggt ggccgccagg gtgaaggcgg tgaggccgaa gagcaccagg cgggtttccg
     1321 gcgaggtgat ggaatagccc agcacgcgct tgaagtcggt gaagccgttg ttgccgccga
     1381 agccggtctc gttgcggaag aacagcagca tcgccgcgta ggtgagggcc tgggtgatga
     1441 tcgagaaata cacgcccttg atccgcgagc ggaaggcgaa gtagccgaac acgaaggcca
     1501 ccagcgccgg cacgcccacc accagcaggg cgcaccacag gaagctgtcg ctgccgctcc
     1561 agtaccaggg cagctccttc cagtcgagga acaccatgaa atccggcaga tcgctctggt
     1621 agctgccgtc gcggccgatc tggcgcatca ggtacatccc gaaggcgtag ccgccaaggg
     1681 cgaagaacag cccgtggccg agggacagga tgccgccgaa gccccagatc aggtccatcg
     1741 ccaccgccac cagggcgtag cacatgatct tgccgatcag ggtgatggcg taggcggaca
     1801 cgtggaaggg gctgtccggc gacacggtca ggtgcagcac cggcaccagc accagggtca
     1861 ccgccaggaa cagcccgagg gccagccagc ccttggcgtc gagggccgcc aggatgcgga
     1921 gcgtgagggg tttgtgcatg gccgggctct cagttgtcga cgaagcggcc cttcagggcg
     1981 aacagcccct gcggacgctt ctggatgaag acgatgatga agacgaggac gacgatcttg
     2041 gcgatcaccg cgccggccca gccttccagc agcttggaga cgacccccag accgagggcc
     2101 gcccacaccg cgccggccag ctgcccgacg ccgcccagca ccaccaccat gaaggaatcc
     2161 acgatgtagc cctggcccat gtccggtccc acgttgctga tctgggacag ggccacgccg
     2221 cccagcccgg cgatgccggc gcccagggcg aaggccaggg tgtcgatgcg cgacgtgggc
     2281 acgcccacgc agccggccat ggcccggttc tgggtcacgg cgcgcacgaa catgcccagg
     2341 cgggtccggt tcatcagcgc ccacaccagg gtcagcacga agagggcaaa gccgatgatg
     2401 atgatgcggt tccagggcag catcaggttg ggcatcagct cgatgccacc ggacatccag
     2461 ctggggttgg agacctccac gttctgggcg ccgaagagca cccgcgccgc ctggatcagc
     2521 accagcgaga taccccaggt ggcgagcagg gtctccagcg tgcggccgta cagccagcgg
     2581 atcaccgtgc gttccatcac cacgcccacc agcgccgcgg ccaggaaggc caccggcacc
     2641 gcggcgggca gataccagtc gatggcgtcc gggaagtagg cgcggaacac gctctgcatg
     2701 gcataggcgg agtaggcgcc caccatcagc agctccccgt gggccatgtt gatgacgccc
     2761 atcaccccgt aggtgatggc caggcccagg gcgatgagca gcaggatgct gcccagggac
     2821 aggccggaga agacccgccc gaggttctcg gcaaaggcca ggcgcgattc gatggccttg
     2881 accgccgtca ccgcctcata gcgcaccttc tcgtccgcct ccccctcgac ggtggtgagg
     2941 gcctgcagct ggctgcggat ggtcggcgag gtgctgtccg ccagggcccg cacggccgcc
     3001 agacgcaccg ccggatcggg gctgcccagg gtggcctggg cgtgggcggt ggcgagcagg
     3061 gccttgatgc cggcgtcgct ttccttgtcc agcgcgcggg cgaggagggg cgccaactcc
     3121 ggggtcgcgc cggtctggag cttctgggcg gaatccttgc gcaccgccgg gtcaccgtcg
     3181 aagagacgca gggccgccag ggcgccggcc agctcgccgc ggatgcggtt gttcaccgtc
     3241 accgagtccg ggctgtcggg catcgggatg gcggcgccgc tggcggcgtc gaaggccttg
     3301 tcgtcggcga cgatcaccac cttgtcgccg gccacataga ggctgtcgtc ggccatcgcc
     3361 tggagcaccc gcagggcgtc cgggtcggcg ctctggacga gcgcacggat cgccgccacc
     3421 ttctcggtgg aatcgtcatc ggcgagtttc cgcacctggg ccgcgtccag cgcggcgtgg
     3481 gccgcgaggc tgcaggcgca gagcagcagg aagaggagtc ttttgatcat gaacggtccg
     3541 tccgcagatt ggtgcagtgc aggagagagc ttacggagca ggcccgcagg cttgaatacg
     3601 atgaattgcg taggggccgt tcgcggcggc ggccccgccg gggggcgccg gccgcggcgc
     3661 atcagtaggt cttgtaaggc aggaacttgc cgctcatgac gatcttcacc cggtcgccct
     3721 tggggtccgg ttcgcgggtc aggtccatgt ggaaatcgat ggcgctcatg atcccgtcgc
     3781 cgaattcctc gtggatcagg gccttgaagg tggtgccata cacattcacc agctcgtaga
     3841 agcggtagat cagcggatcg gtgggcacgg cggtgggcag gctgcccttg tagggcacga
     3901 tctgcagctg ctccacggcc tcgtcgggca ggtcgagggc ggcgcccacg gcctgggcct
     3961 gggcggcgct gagggtcatc tggccgagca gggcggcggt cgtccattcc ttgctctggc
     4021 cgacgatctc ggcgaggcgg gcccagctga gctgcttctt cagcttgcgg acgaggatca
     4081 ggtcggtgac atcttcacgc ttcatgggga ttctccggaa ttgggcgggg gacggcgggc
     4141 ggtgcggggc tgcgggcgcg tcaggcacga caaaacgggg gccgcagccc ccgtttcgtg
     4201 cgacgctcac gacatcggac gggattactt gccttccggc tcgtccttct tcttgtcgtt
     4261 gccggggatg taggggctcc acggggccgc gcggaccggg ccgggggtct tccacaccac
     4321 gttgaactgg ccgtccgcct tcacttcgcc gatgaacacc ggcttgtgca ggtggtggtt
     4381 cttctcgtcc atcttggcga cgaagcccga cggcgccttg aaggtctggc ccgccatggc
     4441 ggcgatgacc ttgtccacat cggtgctctt ggccttctcg acggcctggg cccacatgtg
     4501 gatgccgatg taggtggctt ccatcgggtc gttggtcacc accttgtcgg cgttgggcag
     4561 cttctgcttc ttcgcccagt ccttgtacat cttcacgaac ttggtgtttt ccgggttctt
     4621 cagggacatg aagtagttcc acgccgccag gtggcccacc agcggcttgg tatccacgcc
     4681 gcgcagctcc tcttcaccca ccgagaaggc caccaccggc acgtccgtcg ccttcaggcc
     4741 ggcgttgccc agttccttgt agaagggcac gttggagtcg ccgttgatcg tggacaccac
     4801 cgcggtcttc ttgccctcgg acgcgaactt cttgatcttg gcgatgatcg tctgatagtc
     4861 gctgtgaccg aagggcgtgt agtcctccat gatgtcctcg tccttcacac ccttgctgtg
     4921 caggaaggcg cgcaggatct tgttggtggt gcgcggatac acgtagtcgg tgcccagcag
     4981 cacaaagcgc ttggcctcgc caccgtcctt gctcatcagg tattccaccg ccggaatcgc
     5041 ctgctggttg ggcgcggcgc cggtgtagaa gacgttcttc tcgagctctt caccctcgta
     5101 ctgcaccggg tagaagagca ggccgttgag ttccttgtag accggcagca ccgacttgcg
     5161 ggacaccgag gtccagcagc cgaaggtcac cgccaccttg tccttggtca gcagctggcg
     5221 cgccttttcg gcgaacagcg gccagttgga ggccgggtcc accaccaccg gctccagctt
     5281 cttgcccatc acgccaccgg ccttgttgat ctcgtcgatg gtcatcagcg cggtttcctt
     5341 cagcgccgtc tcggagatgg ccatggtgcc ggacagggaa tgcaggatgc ccaccttgat
     5401 ggtgtcggcc gcctgcgcgg caaaggacgc gaaaccggcg gtggccatcg ccagggacag
     5461 ggcggtggcc ttgatgaagg tgcgacgatt gctcatggaa gctcctcatt cgaaagggga
     5521 atcgggtggc accgggcggt gcgacacgac gccagagtaa ggagcggggg aggacggggg
     5581 aatacgtcag attgcgtagg ggtgcgtagg gggagatgcc tgggcttcag agcttatggc
     5641 tggcacggcc gacatcgtcg aattcggcga tgtcgtcgtg gagggcgccc agatcctgct
     5701 ccgccccctc ggtgagcagg acttcgagcc gggcagccgt gcgcgccctc agtcggaggc
     5761 gcgcttggcg cggaggcggg caacaacgtc ggccacaggc ttgagcttgc ccgccaccgc
     5821 ctcccgatgg cccaacgcca ggattttctg caaggccagt gtttcttgcg tctcttcgaa
     5881 cgaggcgatg tcctgcagaa cagccttcgc ttcgcagttt tgggtgatga ccatcggttc
     5941 gcgctgctcc gcgaggtgcg tgagaacctc ggcagcgttg gttatgagat cgctgatggg
     6001 cttgatctgt gatgagtcgc gcacgggcct gctccggaca ggccggcgat ggcggtgatt
     6061 taactcagag agtgggccgg attcgggcga ggtcatcgtt gtgtggcgga atgtgctgaa
     6121 aacggcgctt tttcgaggag cccagagatt gatcaggatt gtcgccgcgt gaaccatttg
     6181 acaagcttgc tgcttccaat tgcaccacca acgccaccaa tcaatccgcc gattgcggtt
     6241 ccgactccgg ggaaaaacgc tgttccaatc gctgccccgg ctatagcccc gccaagcccc
     6301 cctgagtttg tagctgcact ttcggcggtg cgttttttgg cttcggcaat atcaatcttg
     6361 cctgttgaat atttgtggat agtgatagaa gtttcaatgg cgaatactgc aagcgtaatt
     6421 ggcgcagcac aacgagccac acttttcacg gcttgagata ccgttgcatt agcaacagag
     6481 gcggttgcat tagcagtaac gcgtgcagtt tgtgcgatgg tcgcatctgc ggcgggtgcc
     6541 gtaacctgga tcatcctcgc agctttctcc cccatcgaaa tgtgcattgc agcttgttca
     6601 gcaatggccg tcggaattgg gagttttgat gtggtcaggt agttggacac gattttagcc
     6661 cacgccacgt tccggtctgc tgcggcaacc tgccccattg acacaaaacc atctgctgcg
     6721 cggcctgcca ttttgtaaag ggctttcgcg gtttcattgt tcatctgacc aatactagtc
     6781 atcatcattt tcatttacct ctttttgtta cgctctagct tggttcgttg tgttgatgat
     6841 taggcaacag agggcaatca agacgaagct tgaaaatgca aaaaatgcca atccaattca
     6901 aggcgtcacc attgaggctt ttgcaagggc cccgttgtca accaaaacga agtcaaaatt
     6961 agcataatca aacccctgat ttagcgccac attcggcaca gaacttttgg tctggcgtca
     7021 ttgcagatcc acattggctg cacgaaggaa attctgtcac cttcgccttt tgacgcatgt
     7081 atgcaatctg gacaacgcat gggacggtaa cccggtaggc tgggcttgcc agatcgaaag
     7141 ccgtccagat tgcggtgatt gcccaaccga taggtcccgc aataccggcc aggccccgca
     7201 tgaatgcagc attcacgccc aatgcaaggc cccgcccaat cagagtagtc gcaactgcgt
     7261 tagcaactgt cgtcgcgaga atgtatgaac tgaacccacc agctagaatg ctcgcctgta
     7321 gcgctgcaat tgcgccaggg ccgaatccaa caattttacc gccggtcaat ttactgaaga
     7381 atgacttttg ctcctctggc ttcatttgtt ccataccttt gactgctacg gcgaggagaa
     7441 ttttcagttc gatctgttca gcagaatcag tcttctcgat tttgaccttc atatggtcag
     7501 ccacatcatc caatatctcg cgatatacaa caccgctgcc tcggaagaaa tttactatcg
     7561 agttaccacc aaactcttgg acttccttga taagtaagtc actgttgttc ttgagggtgg
     7621 cgcaacgctt gaaggtatcg atctgggtgc ggatcgtcgt atcgagggaa atgcgaccgt
     7681 tatttctatc agttatgaag tctgcgataa tagcaagatc gctttcttcg gcagcttgaa
     7741 tgatcttaat gagatcgtga tcggtagtga tgtagttagc cattatgtgt cctaaaatta
     7801 ggtttgagat atttcgcggt ccccgagaat tgccccaagg gcaatggcaa acattcgtgc
     7861 atctgatccc tgtcttttta ctgccgcttg ggccattgca gtcatagcaa ggattccttt
     7921 ttgctggcgt tcgcgctccg cgtctgaata gttttcgccg gtcaattcgt tcataaacct
     7981 cagtccggcc atggcagcgt cgtcgatttt ctggagcatt gaagctttag aaagacaatc
     8041 aaccaatgtt cgttccttgt cgccatctct gtttttgagc atttctaatg ttccacacgc
     8101 ttggatcaaa atgctccagt ctcgctgggt catcagcaga aacgccgggc tgacattcag
     8161 tgcatgacca agcttgcaca aagtttcgag gtcgggttta gcatcttggg gatcattgga
     8221 ggttagcttg gtgatcgtgc cctttgaaag attgctcaac tcggcaagct tgcctttgtt
     8281 gatatgcgtt agatgattct cggatacacc cccagcaact ctactcgttt ttacgagatt
     8341 ttctacaaaa gcctcttgga cagcgtaata gagcgcttgg tggcagttat cgagatcgtt
     8401 gtcgtttttc atttcttggt ccaaattgat cgcggatgtg ttttttccgc gttggaggta
     8461 ctgtacccgg ttcgatttcg ccggggaata tagctcaatc atataggata ctgaggtcaa
     8521 aataatcgca ttggagtgcg atttcgcgtc tggttttgta tagtggagac gcactttcag
     8581 tccaccccct tccccacaac tcaccccgcg tgcgtcctgc gcatctgttc aactgtcgat
     8641 cagttgcgat ccagtgatcg accgggagcg tgtgtcatgc cctgttccag acctgtcgaa
     8701 ggcttcaatc gtgccggcgc accctcgtgg gcagggcgcc gaggggctcc gagtggcatg
     8761 catggggtcg gcgacgggat gcttgacatc caaaatggca tgcatggcaa gtaacttgcc
     8821 atgcgtgggt aaaaatcact catgaatgag tattttttgg tcatgattgc ctcattttta
     8881 ggcgacgctg cctatttttt tggcagcgtc gcctaatttt tatgcacagt tgcctaaaaa
     8941 ttaggcggcg ttgcctaaat tttaggcaat catgacaagt ttcaatccga tcatggaaag
     9001 taacttgtca tgaatgagtg tccggaaccc atgatcgcc
//
LOCUS       DAOQMM010000129         8890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_3884_length_8890_cov_43.678664, whole genome shotgun sequence.
ACCESSION   DAOQMM010000129 DAOQMM010000000
VERSION     DAOQMM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3884_length_8890_cov_43.678664"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /gene="purL"
                     /locus_tag="PLH85_15535"
                     /gene_synonym="purI"
     CDS             complement(<1..1997)
                     /gene="purL"
                     /locus_tag="PLH85_15535"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNB66031.1"
                     /translation="MSEILKLRGAPALSASRLARLTDSVKTVLPRLRGLAAEHWYFVE
                     VSAPLSAGQVERLVDLLGAHPEGDAPSGSLLLVTPRLGTISPWSSKATDIAKQCGFDT
                     IVRIERGTALSFDLRGGLDARARESVLPAIHDRMTESVLDNPDAAHALFHHYTPQPLS
                     TVAVVEQGRDALVVANAALGLALSEDEIDYLVDNFTRVGRNPTDVELMMFAQANSEHC
                     RHKIFNADWIIDGRPEEKSLFGMIRDTHKAHPQGTVVAYSDNASVIEGATVPRLYPDA
                     SGAWIYRDELTHILAKVETHNHPTAISPFPGASTGSGGEIRDEGATGRGSKPKAGLAG
                     FSVSNLEIPGFEQPWEQAYGKPERIASPLDIMIEGPLGAAAFNNEFGRPNLTGYFRTF
                     QQSVQDEVRGYHKPIMIAGGLGSIQAEQSHKVQFGPGTLLIQLGGSGMLIGLGGGAAS
                     SMATGTNTADLDFASVQRGNPEIQRRCQEVIDACWQKGADNPILSIHDVGAGGLSNAF
                     PELADSAGLGARFELREVHIEEPGMSPREIWSNESQERYVLAIAPESLAVFQALCERE
                     RCPFAVVGTASDDGRLVVSDRHFGNAPVDMEMQVLLGKPPKMTRNVSTRQVFVPPFDV
                     TDISLDDACRRVLRMPAVASKNFLITIGDRSVGGMTARDQMV"
     gene            2156..2953
                     /locus_tag="PLH85_15540"
     CDS             2156..2953
                     /locus_tag="PLH85_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB66032.1"
                     /translation="MSSADPAPSDLPSWPWIALPALILALLAGLMAADGLNRSAFLNL
                     NAAAAGLAPAGFWAGLSVTGSVLGAFAVLAPTLKTRPRWLASALLAAPPALLFSQGGK
                     RLFDEMRPAAEFAAGSYHQIGVRLYVDAFPSGHATTAFVVAAAVLLAWPDAPRRALTW
                     GLLLLAACLVAFSRIAVGAHWPADVAAGACGGWLCGALGAALTERLRFWQKDATIRLM
                     AGIALIASLALLLVNVGYPEARLYQIGLGAWGIGGAAAALMRERSAA"
     gene            2950..3921
                     /locus_tag="PLH85_15545"
     CDS             2950..3921
                     /locus_tag="PLH85_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB66033.1"
                     /translation="MTLKRLFAVALSLALIGWLAADGRWRGVGEVFARVPLSVLAVSA
                     LGFGLSYSLRALRVWDEFRRHASGRFGACLRIVLIHSAMINVLPFRGGEAAFPLLLRQ
                     TFGVPLPRALASLFWFRLQDAFAVMGVAALVWPGLPTLLKLAGAVSVFALAWWLPRWA
                     RAPHAWADHGGLTAKLAKLRDAFAESTRHARFGWLWTLTNWSVKLAAQAWLLAALIPA
                     ALDVGAAGALGAELAAILPVQGVAGFGTYEAGAAAALLPSGVALATGLQVALALHLFV
                     IAVSVSGGALAWLVPVAAPAEASAPDADPSADVQSASSAADSTRPAP"
     gene            3959..5017
                     /locus_tag="PLH85_15550"
     CDS             3959..5017
                     /locus_tag="PLH85_15550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB66034.1"
                     /translation="MTDTALSSPLPAAAPDIPADLPPHRLSVVVPLYNEEDNVDPLLE
                     RIHLALGPYPYPWEAVIVDDGSSDNTVPRLEAAAKRYGPHVRIVALMRNFKQTAAMQA
                     GLDAARGDVIVTMDGDLQNDPIDIPRMVGRLLREDLDLVAGWRKNRKDGMILRKIPSK
                     IANRLIARITGVHLQDYGCSLKAFRATAIKNVRLYGEMHRFIPAWLATVTTPRKIAQE
                     VVTHHARVFGQSKYGISRTFRVILDLVFVYFFMRFRTRPGHFFGGIGLGLGALGVLIL
                     GYLSLLKLFTGASIGTRPMFFGGFFFVIAGVQMVTSGVLAELLTRVYFESGTSLAYVA
                     RETAPLADEQGWSGGPKA"
     gene            5028..6596
                     /locus_tag="PLH85_15555"
     CDS             5028..6596
                     /locus_tag="PLH85_15555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB66035.1"
                     /translation="MNGLASGRLAGSTLGRLILALPLFAFLLRLGGAPLFDVDEGAFS
                     EATREMFERGDFLFTYLNGEPRYDKPILIYWLQALGYLIFGPSEWAFRLPSALAAIAW
                     SYATWLFARRRIGEDAALVALAVAASSLGPFAIGRAATADGLLNCLLALSLFDLWRHL
                     ESGERAPLLRAYLWIGLGALTKGPVALIVPGAVTCLYCLSRGEGLRWLRMIAHPAGWA
                     ILLLLIAPWYAYALHRHGQDFINGFLLKHNVQRFSGALEGHGGSAAYYLVAVPLLLLP
                     WSGLLGVALSRLRSDVAHPLRRFLWIWFGFVLVFFSLSGTKLPHYVLYGCTPLFVLIG
                     LHADGARRIWPHLLVPGLLMLFCIALPGVLDHLAHGTAGDGYYRAQFSRALAEAGPLY
                     YGLTAGALLLWLAFVLFIRVPLIHKLAVTAILQVGLLAGVVVPYAGELAQGPVKAAGL
                     VARDLHEPVIFWNFSTVPSFSVYRQAVTHLAEPAPGELALTRIDRLPPDVPVETLFQR
                     GGVVLVRRLPAPKP"
     gene            6593..8155
                     /locus_tag="PLH85_15560"
     CDS             6593..8155
                     /locus_tag="PLH85_15560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB66036.1"
                     /translation="MTAAPHSSGPLQRSHYAALLAVIATVLTAYRILVIGHLGIDLYV
                     DEAYYWGWSKALDWGYFSKPPLIAALIAASTALFGDGLLAIKLPSLLLYPATAAALFV
                     LGERLHSVRAGFWAGLIFMTMPLVSALGLFVSTDAPLLLCWALALIALLKAVDAEQGW
                     GAWLAWGAAVGVGLMAKYTMAAFLPSALLLMLLEPAGRRWLCRPQPWLALLLALAIFS
                     PNLYWNWVHHFPTFRHTAEITEVGHRGWHPGELGEFIGAQWLSLGPLMGILLAWALAR
                     SRRIGQDRAGRVLLIFILPLLTLVSLQALTGRANGNWAAPIFVAACVLIPAYFLAERR
                     RWLVAGVALNLVGSLFVYHWPDVARLADIPLTAKTDPYKRARGWTRLADGVAPYLAAH
                     PDAILVGEDREITAHLVYRLHPRDYAAWNPGGRPPIDHYQLVTHLDDQLGRDILYVGN
                     VPELPGMEPYYAAIEPLGKVVVPVHTDFRRTVYVFLLKGFKGYKPSAASAPTIAPAPT
                     AESSTPPVVSRP"
     gene            complement(8578..>8890)
                     /locus_tag="PLH85_15565"
     CDS             complement(8578..>8890)
                     /locus_tag="PLH85_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66037.1"
                     /translation="LAHILYSRAQIRLQRGDHHDSGLHAIYEDLARAYAFLVKLGRPD
                     GIGTAGALLAQVLAMEGSRSEALQVLETAEAAWRKIGQMQQVARVAELRQTIEGRGDG
                     Q"
BASE COUNT         1136 a         3453 c         2827 g         1474 t
ORIGIN      
        1 accatctggt cgcgggcggt catgccgccc acggaacggt cgccgatggt gatgaggaag
       61 ttcttgctgg ccaccgccgg catgcgcagc acgcgccggc aggcgtcgtc caggctgatg
      121 tcggtcacgt cgaagggcgg cacgaagacc tggcgggtgg acacgttgcg ggtcatcttg
      181 ggcggcttgc cgaggagcac ctgcatctcc atgtccaccg gcgcgttgcc gaaatgccgg
      241 tccgacacca ccaggcgacc gtcgtccgac gccgtgccca ccaccgcgaa ggggcaacgc
      301 tcccgctcgc agagggcctg gaagaccgcc aggctctccg gcgcgatggc cagcacgtag
      361 cgctcctggg attcgttgga ccagatctcc cgcgggctca tgcccggctc ctcgatatgc
      421 acctcgcgca gttcgaagcg cgcgccaagg ccggcggaat cggccagctc ggggaaggca
      481 ttggaaaggc cgccggcgcc cacgtcgtgg atggacagga tcgggttgtc ggcccccttc
      541 tgccagcagg cgtcgatgac ctcctggcag cgccgctgga tttccggatt gccgcgctgc
      601 accgaggcaa agtccaggtc ggcggtgttg gtgccggtcg ccatggatga agcggcgccg
      661 ccgcccaggc cgatgagcat gcccgagccg cccaactgga tcaacagcgt gccggggccg
      721 aactgcacct tgtgggactg ctcggcctgg atgctgccca ggccgccggc gatcatgatg
      781 ggcttgtggt agccacgcac ctcgtcctgc acgctctgct ggaaggtgcg gaaatagccc
      841 gtcaggttgg gacggccgaa ctcgttgttg aaggccgccg cgcccagcgg gccttcgatc
      901 ataatgtcga ggggcgacgc gatgcgctcc ggcttgccgt aggcctgctc ccagggctgc
      961 tcgaagccgg ggatctccag gttggacacc gagaaaccgg ccagaccggc cttcggcttg
     1021 gagccacggc cggtggcgcc ctcgtcgcgg atctcgccgc cggagccggt ggacgcgccg
     1081 gggaagggcg agatggcggt cgggtggttg tgggtctcca ccttggcgag gatgtgggtc
     1141 agctcgtccc ggtagatcca cgcgccggag gcgtcgggat acaggcgcgg cacggtggcg
     1201 ccctcgatca ccgaggcgtt gtcggaataa gctaccacgg tgccctgcgg gtgggccttg
     1261 tgggtgtcgc ggatcatgcc gaagagggat ttctcctcgg gccggccgtc gatgatccag
     1321 tcggcgttga agatcttgtg ccggcagtgc tcggaattgg cctgggcgaa catcatcagc
     1381 tccacgtccg tcgggttgcg gcccacacgg gtgaagttgt ccaccagata gtcgatctcg
     1441 tcctcggaca gggccagacc cagtgcagcg ttggccacca ccagcgcgtc gcggccctgc
     1501 tcgaccaccg ccacggtgct caggggctgg ggcgtgtaat ggtggaacag ggcgtgggcg
     1561 gcgtccgggt tgtccagcac cgactcggtc atgcggtcgt ggatggccgg cagcacggac
     1621 tcccgcgcac gggcgtcgag gccgccgcgc aggtcgaagc tcagggccgt gccccgctcg
     1681 atgcgcacaa tggtgtcgaa gccgcactgc ttagcgatgt ccgtcgcctt cgacgaccag
     1741 ggcgagatcg tgcccaggcg cggcgtcacc agcagcagac tgcccgacgg cgcatccccc
     1801 tcgggatggg cgccgagcag atccaccagc cgctccacct gcccggcgga caggggcgcc
     1861 gacacctcca cgaaatacca gtgctcggcc gccagcccgc gcaggcgcgg caggacggtc
     1921 ttcacagagt cggtgaggcg ggcaaggcgg gaggcggaca gggcgggcgc accgcgcagc
     1981 ttgagaattt cggacatggc aaggatggcg cgtagttgga accgacccgg cgcgacggcc
     2041 gggacacaca ggaccgggca ttatacccga gccccgcccc gcgccttgcg acgcatgtcc
     2101 gccttgcccc cggcttcggg tacactcccg ccttttgctg ccccacccgc gcctgttgag
     2161 ctcagccgac cccgctccct ccgacctgcc gtcctggccc tggatcgccc tgccggcgct
     2221 gatcctcgcc ctgctggccg gactgatggc ggcagacggc ctcaatcgca gcgccttcct
     2281 gaatctcaac gccgcggcag ccgggctggc gcccgccgga ttctgggccg gcctgtccgt
     2341 caccggctcg gtgctcggcg ccttcgccgt cctcgccccc accctcaaga cccgcccgcg
     2401 ctggctcgcc tcggccctgc tcgccgcgcc gccggcgctc ctcttcagcc agggcggcaa
     2461 gcgcctcttt gacgagatgc gccccgccgc cgaattcgca gccggcagct accaccagat
     2521 cggcgtgcgg ctttacgtgg acgccttccc gtccggccac gccaccaccg ccttcgtcgt
     2581 cgccgcggcg gtgctgctgg cctggccgga cgccccgcgg cgggccctca cctggggact
     2641 gctcctcctg gccgcctgcc tggtggcctt ctcgcggatc gccgtagggg cccactggcc
     2701 cgcggatgtg gcggccggcg cctgcggcgg ctggttgtgc ggcgccctcg gcgcggccct
     2761 cacggaacgc ctgcgcttct ggcagaagga cgcgaccatc cgcctgatgg ccggcatcgc
     2821 cctcatcgcg agcctggccc tgctgctggt gaacgtgggc tacccggaag cccgcctgta
     2881 ccagatcggc ctgggcgcct ggggcatcgg cggggctgcc gccgccctga tgcgtgagcg
     2941 gagcgcggca tgacgctcaa acgcctcttc gccgtcgccc tgtccctcgc cctcatcggc
     3001 tggctcgccg ccgacggccg ctggcgcggc gtgggtgagg tcttcgcgcg ggtgcccctg
     3061 tcggtgctgg ccgtgtccgc gctgggcttc ggcctgtcct acagcctgcg tgccctgcgc
     3121 gtgtgggacg aattccgccg ccacgccagc ggacgcttcg gcgcctgcct gcgcatcgtg
     3181 ctgatccaca gcgccatgat caacgtgctg cccttccgcg gcggcgaggc ggccttcccg
     3241 ctgctgctgc gccagacctt cggcgtgccc ctgccgcggg ccctggcgtc cctgttctgg
     3301 ttccgcctgc aggacgcctt cgccgtgatg ggcgtggccg cgctggtgtg gcctggcttg
     3361 ccaaccctcc tcaagctggc cggcgccgtg agcgtgttcg cgctggcctg gtggctgccc
     3421 cgctgggcgc gggcgcccca cgcctgggcc gatcacggcg gcctcactgc caagctcgcc
     3481 aagctgcgcg acgccttcgc cgagagcacc cgccacgccc gcttcggctg gctgtggacg
     3541 ctcaccaact ggtccgtcaa gctggccgcc caggcctggc tgctggccgc gctgatcccc
     3601 gccgccctcg acgtgggtgc cgccggcgcg ctgggcgccg agctcgcggc gatcctcccg
     3661 gtgcagggcg tggccggctt cggcacctac gaggccggcg ccgccgcggc gctgctaccc
     3721 agcggcgtcg ccctcgccac cggcctgcag gtggccctgg ccctgcacct gttcgtgatc
     3781 gcggtgtccg tcagcggcgg cgccctcgcc tggctggtgc ccgtcgccgc cccggccgag
     3841 gcctccgcgc cggacgccga cccctctgcc gacgtacaat ccgcgtcgtc cgccgccgac
     3901 agcacacggc ccgcgccctg agcgcccgcc tgccgcggcc ttttccgaga tttccgccat
     3961 gaccgacacc gccctgtcct ccccgctccc ggccgctgcg cccgacatcc cggccgacct
     4021 gccgccccat cgcctgtcgg tggtggtccc gctctacaac gaggaggaca acgtggaccc
     4081 gctgctggag cgcatccacc tggccctcgg cccctacccc tatccgtggg aggcggtgat
     4141 cgtggacgac ggcagctccg acaacaccgt gccgcgcctc gaagccgccg ccaaacgcta
     4201 cggtcctcat gtgcgcatcg tcgccctgat gcgcaacttc aagcagaccg ccgccatgca
     4261 ggccggcctg gacgccgccc gcggcgacgt gatcgtcacc atggacggcg acctgcagaa
     4321 cgaccccatc gacatcccgc gcatggtcgg ccgcctgctg cgggaagacc tggacctggt
     4381 cgccggctgg cgcaagaacc gcaaggacgg gatgatcctg cgcaagatcc cgtccaagat
     4441 cgccaaccgc ctgatcgcgc gcatcaccgg cgtgcatctg caggactacg gctgctccct
     4501 caaggccttc cgcgccaccg ccatcaagaa cgtgcgcctg tacggcgaga tgcaccgctt
     4561 catccccgcc tggctggcca ccgtcaccac gccgcgcaag atcgcccagg aggtcgtcac
     4621 ccaccacgcg cgggtcttcg gccagtccaa atacggcatc tcgcgcacct tccgggtcat
     4681 cctcgacctg gtcttcgtgt acttcttcat gcgcttccgc acccggccgg gccacttctt
     4741 cggcggcatc ggcctgggcc tcggcgccct gggcgtgctg atcctcggct acctgagcct
     4801 gctcaagctg ttcaccggcg cctccatcgg cacccggccc atgttctttg gcggcttctt
     4861 cttcgtcatc gccggcgtgc agatggtcac ctccggcgtg ctggccgaac tgctcacccg
     4921 ggtgtatttc gaatccggca ccagcctcgc ctacgtggcc cgcgaaaccg ctccgctggc
     4981 ggacgagcag ggctggtccg gcggccccaa ggcctgatcc ccgcccgatg aacggcctcg
     5041 ccagcggacg cctcgccggg tccaccctcg gccgcctgat cctcgccctg cccctcttcg
     5101 ccttcctgct gcgcctgggg ggcgcgcccc tcttcgacgt ggacgaggga gctttctccg
     5161 aggccacacg ggagatgttc gagcgcggcg acttcctgtt cacctacctc aacggcgaac
     5221 cgcgctacga caagcccatc ctcatctact ggctgcaggc cctgggctac ctgatcttcg
     5281 gccccagcga gtgggccttc cgcctgcctt cggcgctggc cgccatcgcc tggagctacg
     5341 ccacctggct gttcgcccgc cgccgcatcg gcgaagacgc ggccctggtg gcgctggcgg
     5401 tcgccgcctc gtccctgggc cccttcgcca ttggccgcgc cgccaccgcc gacgggctgc
     5461 tcaactgcct gctggccctg tccctcttcg acctgtggcg ccacctggag agcggcgaac
     5521 gcgcgcccct gctgcgcgcc tacctgtgga tcggcctggg cgccctcacc aagggaccgg
     5581 tcgcgctgat cgtccctggc gccgtcacct gcctgtactg cctgtcccgc ggcgagggcc
     5641 tgcgctggct gcgcatgatt gcccacccgg ccggctgggc gatcctgctt ttgcttatcg
     5701 ccccctggta cgcctacgcc ctgcaccgcc acggccagga cttcatcaac ggcttcctcc
     5761 tcaagcacaa cgtgcagcgc ttctccggcg ccctggaagg ccacggcggc agcgccgctt
     5821 attacctggt ggccgtgccc ctgctgctgc tgccctggag cggcctgctg ggcgtcgccc
     5881 tgagccgcct gcgcagcgac gtggcgcacc ccctgcggcg cttcctgtgg atctggttcg
     5941 gcttcgtgct ggtgttcttc tccctctccg gcaccaagct gccccactac gtgctctacg
     6001 gctgcacgcc cctcttcgtg ctcatcggcc tgcacgccga cggcgcccgg cggatctggc
     6061 cgcacctgct ggtgccgggt ctgctgatgc tcttctgcat cgccctgccc ggcgtgctgg
     6121 accacctcgc ccacggcacg gcgggcgacg gctactaccg cgcccagttc agccgcgccc
     6181 tcgccgaagc cggaccgctc tactacggcc tcacggccgg cgccctgctc ctgtggctgg
     6241 ccttcgtgct gttcatccgc gtgcccctga tccacaagct cgccgtcacc gccatcctcc
     6301 aggtgggcct gctggcgggg gtcgtggtgc cctacgcggg cgagctggcc caaggccccg
     6361 tcaaggccgc cggcctggtg gcgcgggatc ttcacgagcc ggtgatcttc tggaatttct
     6421 ccaccgtgcc cagcttcagc gtctatcgcc aggccgtcac ccacctggcc gagccggccc
     6481 ccggcgagct ggccctgacc cgcatcgacc gcctgccgcc ggacgtgccc gtggagaccc
     6541 tgttccagcg cggcggcgtg gtgctggtgc gccgcctgcc cgcgcccaag ccatgaccgc
     6601 cgcaccccat tcctccggcc cgctgcagcg cagccactac gccgccctgc tggccgtcat
     6661 cgccaccgtc ctcacggcct accggatcct cgtcatcggc cacctgggca tcgacctcta
     6721 cgtggacgag gcctactact ggggctggtc caaggccctg gactggggct acttctccaa
     6781 gccgccgctc atcgccgccc tgatcgccgc cagcaccgcc ctcttcggcg acggcctgct
     6841 cgccatcaag ctgccctccc tgctcctcta tccggccacc gccgcggccc tcttcgtgct
     6901 gggcgaacgc ctccactcgg tgcgcgccgg cttctgggcc gggctgatct tcatgaccat
     6961 gcccctggtc tccgccctgg gcctgttcgt gtccaccgac gccccgctgc tcctgtgctg
     7021 ggccctggcc ctcatcgccc tgctcaaggc cgtggacgcg gaacagggct ggggcgcctg
     7081 gctggcctgg ggtgccgcgg tcggcgtcgg cctgatggcc aagtacacca tggccgcctt
     7141 cctgccctcc gccctgctgc tcatgctcct ggagccggcc gggcgccgct ggctgtgccg
     7201 gccccagccg tggctcgccc tgctgctggc cctggccatc ttcagcccca acctgtactg
     7261 gaactgggtc caccacttcc ccaccttccg ccacaccgcc gagatcaccg aagtcggcca
     7321 ccggggctgg caccccggcg aactgggcga attcatcggc gcccagtggc tgtccctggg
     7381 gcccctgatg ggcatcctgc tggcctgggc cctggcccgc agccggcgca tcgggcagga
     7441 ccgcgccggt cgcgtgctgc tcatcttcat cctgcccctg ctgacgctgg tcagcctcca
     7501 ggccctcacc gggcgcgcca acggcaactg ggccgcgccc atcttcgtcg ccgcctgcgt
     7561 gctgatcccc gcctacttcc tggccgagcg gcgccgctgg ctggtggccg gcgtggccct
     7621 caacctggtg ggctccctct tcgtctacca ctggcccgac gtcgcccgcc tggccgacat
     7681 ccccctcacc gccaagaccg acccctacaa gcgcgcccgc ggctggaccc gcctcgccga
     7741 cggcgtcgcc ccctacctcg ccgcccaccc ggacgccatc ctcgtcggcg aagaccggga
     7801 gatcaccgcc cacctggtct accgcctcca cccccgcgac tacgccgcct ggaaccccgg
     7861 cggccgcccg cccatcgacc actaccagct cgtcacccac ctggacgacc agctcggccg
     7921 cgacatcctc tacgtgggca acgtgccgga actccccggc atggagccct actacgccgc
     7981 catcgaaccc ctcggcaagg tggtcgtgcc cgtccacacg gacttccgtc gcacggtgta
     8041 tgtgtttctg ctgaaggggt tcaagggcta caagccgagc gcagcgtctg cgccgaccat
     8101 cgcgccggca cccacggcgg aaagctcgac gccaccggtc gtctcccggc cctgacaggt
     8161 ctgcgcaggc ctccccagac cctgtgcgcc cggacaacac ggctgttcgc gtcgccgatg
     8221 ccttgtctct gagcatccat caccctgcgg accgccctgc aaggtctgcg cagacgtcca
     8281 cagaccctgt agacgtggac accaaggctg ttgatagccc tttacctttg taagaggcct
     8341 cttaccaatg gaaagggcta tcaatcctca atgtagcggt cgcttacaag actgtggacc
     8401 ggtcgacatc gtctgttgac ccgtccacac acactgtcga cccgtccaca tcgtccgtcg
     8461 accggtcgac agtgtgtgtg caccgatcat catcatccgt ggaccggtcc acacaccgtg
     8521 ttgacggcgc cacaaagtgc gtcgcgctgc ccgacaacgc tgggcacggg cgtatcccta
     8581 ctgcccgtcc ccgcgcccct cgatcgtctg gcggagttcc gccaccctcg ccacctgctg
     8641 catctgcccg atcttccgcc aggcggcttc ggcggtctcc agcacctgga gcgcttcgct
     8701 ccgcgatccc tccatggcca gcacctgggc cagcagggct cccgcggtcc cgatgccgtc
     8761 cggacgcccc agtttcacaa ggaaggcgta tgcacgggcc aggtcttcgt aaatggcatg
     8821 caggcccgag tcgtggtggt cgcccctttg caggcggatc tgggcccggg aatacagaat
     8881 gtgggccaga
//
LOCUS       DAOQMM010000130         8633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_4064_length_8633_cov_46.036838, whole genome shotgun sequence.
ACCESSION   DAOQMM010000130 DAOQMM010000000
VERSION     DAOQMM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4064_length_8633_cov_46.036838"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..558)
                     /locus_tag="PLH85_15570"
     CDS             complement(34..558)
                     /locus_tag="PLH85_15570"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNB66038.1"
                     /translation="MPCETRQLGVWQGDITTLAVDAIVNAANPTLLGGGGVDGAIHRA
                     AGPDLTAYCAGLGGCPTGEARLTPGFRLPARHIIHTVGPIWRGGRAGEAELLATCYRA
                     CFALAIAHDLHSLAFPAISCGVYGYPPEAAARIAVREARTALACAGGPTAVRLVAFDP
                     SMARLLHQALDAPA"
     gene            737..1396
                     /locus_tag="PLH85_15575"
     CDS             737..1396
                     /locus_tag="PLH85_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB66039.1"
                     /translation="MARSRVVAVLSQKGGAGKSTLVMQLAGGLAKVGKKVAVGDLDPQ
                     ETALRWAESAPAEQPFPARVQSLAAAGDLPAAIRGCGKGCEVLLLDCPPSIESPATLA
                     ALQVADVALIPVVPSPTDLWSTRAVEKLVLHVQAGRKGLKARLVPNRVVRTNLAWDVL
                     EVMRDFALPVLGAALSQRNAYAQSAVIGGSVYQLGRAGAEAAREVNMLVAEVLKLTGE
                     R"
     gene            1396..>1843
                     /locus_tag="PLH85_15580"
     CDS             1396..>1843
                     /locus_tag="PLH85_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66040.1"
                     /translation="MNGKTRSALRSTLKKEDAAVAARLPEVTAVAAETPAPVESVGLE
                     SAPAPVVEPTPSAGPAVEDVVPESAAVEADAPAAQAPARPKPVKAPAKPKAAPKAAAS
                     SEAKADVKPAGVSSVEAKAVKVKAPRPTKEGKPEKAPKVAKAVKPVK"
     assembly_gap    1844..1943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1944..2159
                     /locus_tag="PLH85_15585"
     CDS             <1944..2159
                     /locus_tag="PLH85_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66041.1"
                     /translation="RVKVVRDSFSVPKSEHAQLKALREALAKEGRIATKSELLRVGIQ
                     LLAKEPLASLKTLVEGLSVVPKGKSAK"
     gene            complement(2485..4260)
                     /locus_tag="PLH85_15590"
     CDS             complement(2485..4260)
                     /locus_tag="PLH85_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNB66042.1"
                     /translation="MPPVLPSPPFALFDNNLDADGTSGAWLLTDLREEVVVRADGDWR
                     AALDRVDAAARAGAWVTLAATYELGETIEPRLRRPAAAPGAPVLQAWIFARAEWLDDD
                     ACDAVLAALADEEPAACTPLRPTLDAPTYTRAIERIRRYIEEGDCYQVNFTFPLHGQL
                     IGSPAALYRQLRRAQPVRHGAYIVHRDGVILSRSPELFLERNGLCLASEPMKGTAPRS
                     SDPQRLASSAKDRAENVMIVDLLRNDLGRLVPPGGVRVEALCRLDAYPTVWQMSSRIV
                     AEPVHAGLGDIFSALFPCGSITGAPKIRAMEIIRTLEDGPRGLYCGALGWIRPGGDFR
                     FSVPIRTARVAPDGALRLDVGSGIVHDSRPDAEWQECHLKARFLTDLPTDLRLIETLR
                     LEPDGAHRVPFLTEHLERLSSSATALGFAFDATAFRRLLDGVQADSALRIRITLGRAG
                     DLTLETAPLTSGAPGPAPTICLAPDPVRSNDPRLRHKTTARQRYDRALARALADGHFD
                     TLFRNERGELTEGARSNLFILRNGELLTPPLACGLLNGVLRRRLIHTGEAREAVLTLD
                     DLRRADAVFMGNGLRGRVQVRLADT"
     gene            complement(4528..5679)
                     /gene="ppk2"
                     /locus_tag="PLH85_15595"
     CDS             complement(4528..5679)
                     /gene="ppk2"
                     /locus_tag="PLH85_15595"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNB66043.1"
                     /translation="MNDKAAPATPRSNGKAPRPRRAAQGDTAPALNAQGIEAYAVDEA
                     VDAARDSKAAAVSDIVATLAKHDTASIAETLQAILAGASPDDAALLRKALQSEDDVIY
                     SKLPAHPDEELAEGWREGGYPYKNLMSRKAYEKQKYRLQVELLKLQAWVKETGQRVVI
                     LFEGRDAAGKGGTIKRFMEHLNPRGARVIALEKPSETERGQWYFQRYVQHLPTAGEIT
                     MFDRSWYNRAGVERVMGFCSHDEYLEFMRQTPEFERHLARSGVHLVKFWFSVSREEQR
                     RRFKERENHPLKQWKLSPIDLASLDKWDDYTKAKEAMFFHTDTADSPWTVIKSDCKKR
                     ARLNAMRYILHKLPYANKDLEHIGPLDPLLVGRAHVVYERGEHSGGPLI"
     gene            complement(5830..6753)
                     /locus_tag="PLH85_15600"
     CDS             complement(5830..6753)
                     /locus_tag="PLH85_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66044.1"
                     /translation="MTTDLSDSDIPEGTKRIIDGRERVFYSGYWIKTYHVPADTLQAK
                     RQLIGALTRRLFNHVEHGLNVPGSRLNEARQSFEAETDPARRRVKGAMLAGALFNRAA
                     DIFTKLVELQSVGIEIQSDNALMRECGACLQEALGLGRLVLHISGEEGIDELWGEPFK
                     AFSIPLEDFYESRYIKIALTQRDIDRVAATVVECFDPLPCFHGIAAHMLEFANAAKIK
                     TETLRTDPDIFNIWTTFVTAAERLCGHFNDVLPNNDPDTERLVGDGRRLIQQGRDLIS
                     YITRARTPMPKSTTDFIERCKAYRARWDAHF"
     gene            complement(6907..7497)
                     /gene="mobA"
                     /locus_tag="PLH85_15605"
     CDS             complement(6907..7497)
                     /gene="mobA"
                     /locus_tag="PLH85_15605"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNB66045.1"
                     /translation="MDVALITGLILAGGQGRRMGGCDKGLVHLDGKPLIAHILGRFAP
                     QVGPLLINANRNADAYAAFGHPVIADRLAGHAGPLAGLDAGLAACETPFLACTPCDSP
                     CLPEDLVARLADALESRGADAAVARAGGRLHPVFALIRCTTAEALTAYIDGGGRRVET
                     WFNGLHTVTCAFDECPAAFDNLNTLEDLRTHTHTPV"
     gene            complement(7503..8591)
                     /gene="moaA"
                     /locus_tag="PLH85_15610"
     CDS             complement(7503..8591)
                     /gene="moaA"
                     /locus_tag="PLH85_15610"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNB66046.1"
                     /translation="MNQRVIPLADERQLARRPAIPSDRPAPDGRLADRRGRQVRDLRI
                     SVTDRCNFRCVYCMPKSVFDQDYAFLPRNALLSFEEITRIARLFTAHGVRKIRLTGGE
                     PLLRRNLEDLIAQLAALPDMEVTLTTNGVLLPRKARALKAAGLARVTVSLDALDDAIF
                     RAMNDVDVPVADVLAGIAAADAAGLGPIKINMVVKRGLNDREILPMARHFRGSGHILR
                     FIEFMDVGSSNGWRMDDVVPSAEVIRRIGAEFPLEATDPNYTGEVAERWRYVDGGGEI
                     GVISSVTQAFCSTCTRARLSTEGRLYTCLFAQSGHDLRPLLREGCSDEELSAAIGAIW
                     QDRNDRYSELRTAATADLRKIEMSYIGG"
BASE COUNT         1337 a         2582 c         3204 g         1410 t
ORIGIN      
        1 gtgtggggag tatacccagc ccgcgcaggc gggtcaggca ggggcgtcca gggcctggtg
       61 caggagtctg gccatggaag ggtcgaaggc gaccaggcgc acggcggtcg gaccacctgc
      121 gcacgcaagt gccgtccggg cctcgcgcac cgcgatgcgg gcggcagcct cgggcgggta
      181 gccatacacg ccgcagctga tcgccggaaa ggcgaggctg tgcaggtcat gggcgatcgc
      241 cagcgcaaag caggcgcgat agcaggtggc gagtagctcc gcctcgccgg cccggccgcc
      301 gcgccagatc ggaccgacgg tgtggatgat gtggcgcgcg ggcaggcgga agccgggcgt
      361 caggcgcgct tcgccggtcg gacagccgcc gagcccggcg cagtaggccg tgagatccgg
      421 gccggccgcc cgatggatgg caccgtccac cccgccgccg ccgagcaggg tcggattggc
      481 tgcattgacg atggcatcca ccgcgagggt ggtgatgtca ccttgccaga cgccgagctg
      541 acgggtttcg cagggcatgg tggcctccac gggacagagc gtcgattatg cctgtgccgg
      601 ctgcgcccgc ggcaggcgat ggacggcaga atgcgggcgg tgcggcgccc cgtgcggtga
      661 gcgaaccaca gggcgggcgc ttgcgaattg cgggcagtgg tataaaatca tacggtatta
      721 cgcaaacgga ggtcgcatgg cgcgcagccg ggtggtcgcg gtgctgtccc agaaaggcgg
      781 tgccggcaaa tcgacgctgg tgatgcaact ggccggaggg ctggcaaaag tcggcaagaa
      841 ggtcgcggtc ggggatctgg atcctcagga aaccgccctg cgctgggccg agtcggcccc
      901 cgcggagcag cccttcccgg cgcgggtcca gtccctggcg gcggccggcg atctgccggc
      961 ggcaattcga gggtgtggca agggctgcga ggtgttgctg ctcgactgtc cgccctccat
     1021 cgagagcccg gccaccctgg cagccctgca ggtggccgat gtggccctga ttccggtggt
     1081 gcccagtccg accgacctgt ggtcgacgcg ggcggtggaa aagctcgtcc tgcatgtgca
     1141 ggcggggcgc aaggggctca aggcccgcct ggtgcccaac cgggtggtgc ggaccaatct
     1201 ggcctgggac gtgctggagg tgatgcgtga cttcgcgttg ccggtgctcg gcgcggccct
     1261 gtcccaacgg aatgcctacg cgcagagcgc ggtgatcggc ggctcggtgt atcagctcgg
     1321 gcgggccggg gccgaggcgg cacgcgaggt gaacatgctg gtggctgaag tactgaaact
     1381 gacaggagaa cgatgatgaa tggcaagacc cgctctgcgc tgcgctcgac gctcaagaag
     1441 gaggacgccg cggtggcggc ccgcctgccc gaggtgacgg cagtcgctgc cgaaacgccg
     1501 gcgccggtcg aatcggtggg gcttgaatcc gcgccggcgc ccgtggtcga accgaccccg
     1561 tcggcggggc cggccgtcga ggacgtggtg cccgaatctg cggcggtcga ggcggatgcg
     1621 ccggccgcgc aggcgccggc ccggccgaag ccggtgaaag cgcctgcgaa gcccaaggct
     1681 gcgccgaagg cggccgcctc gtccgaggca aaggcggacg tcaagccggc cggcgtgtcg
     1741 tcggtcgagg ccaaggcggt caaggtgaag gcaccccggc ccaccaagga gggcaagccc
     1801 gagaaggcac ccaaggtcgc caaggcggtg aagcccgtga aggnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnncgggtca aggtggtgcg ggactcgttc tcggtgccca
     1981 agtccgaaca cgcccagctc aaggccttgc gtgaagcgct ggccaaggaa gggcggattg
     2041 cgaccaagtc cgaactgctg cgggtgggca tccagctcct ggccaaggag ccgctggcgt
     2101 cgctgaagac gctggtggaa ggtctcagcg tcgtgcccaa gggcaagagc gccaagtaag
     2161 cgcggcggga taccgccatc cggtgtggaa gaaagggcgc tgcggcgccc ttttttcgtt
     2221 gcgctgcctt ccggatcctc ggtgataccg cgcgccggat ccgggcgccc ggtctggaat
     2281 ccgctgcagg tgtggcgggc tgaatcgtct ggcgatatgc gcttgagcgg gcgcgcaggt
     2341 gcctggttgg ggatctccga cgcagtgacg cgcgcagggc ccgccggccg ggcgatcgtc
     2401 cagagcgttg ggatgcgtcg gtacggctct gggggcgtgg acggcagggg cggtccgcgc
     2461 ccgcggtctg gatgcgcggt ggtgtcaggt gtccgccaga cgtacctgca cccgtccgcg
     2521 cagaccattg cccatgaaga cagcgtcggc gcggcgcagg tcgtccaggg tgagcaccgc
     2581 ctcgcgggcc tcgccggtgt ggatcagacg acggcgcagg acaccgttga gcaggccgca
     2641 ggcgagaggc ggggtgagga gttcgccgtt tctcaggatg aacaggttgc tgcgggcgcc
     2701 ttccgtgagt tcgccccgtt cgttgcggaa cagggtgtcg aaatgaccgt cggcgagcgc
     2761 tcgtgcgagt gcccggtcat accgctgccg ggcggtggtc ttgtggcgca ggcgggggtc
     2821 gttgctgcgc acggggtcgg gtgcgaggca gatggtgggg gcggggcccg gggcgccgga
     2881 tgtgagcggg gcggtctcaa gggtgagatc gccggcgcgg ccgagggtga tccggatgcg
     2941 cagggcgctg tcggcctgca cgccgtccag tagacggcgg aaggcggtcg cgtcgaaggc
     3001 gaagcccagc gccgtggcgg agctgctcag gcgctccaga tgttcggtca gaaaggggac
     3061 gcggtgagcc ccgtccggtt cgaggcgcag ggtttcgatc aggcgaagat ccgtgggcag
     3121 gtcggtgagg aagcgcgcct tgagatggca ttcctgccac tccgcgtcgg ggcgggagtc
     3181 atggacgatg ccgctgccca cgtccagccg cagggcgccg tccggggcca cgcgggcggt
     3241 gcggatcgga acgctgaagc ggaagtcgcc gcccggccgg atccagccca gcgcgccgca
     3301 gtacaggccg cgggggccat cctccagcgt acggatgatc tccatcgcgc ggatcttggg
     3361 cgcaccggtg atcgagccgc aggggaagag ggcgctgaag atgtccccca gaccggcgtg
     3421 cacgggttcg gcgacgatgc gcgagctcat ctgccagacg gtgggatacg cgtcgaggcg
     3481 gcacagggct tcgacccgta cgccacccgg tggcacaagg cgtcccagat cattgcgcag
     3541 gaggtccacg atcatcacgt tttcggcccg gtccttggct gaggaggcca gccgctgcgg
     3601 atcgctgctg cgcggcgcgg tgcccttcat gggttcgctg gcgaggcaca ggccattgcg
     3661 ttccaggaac agctccggtg agcgggacag gatgactccg tcgcgatgga cgatgtaggc
     3721 accatggcgg accggctggg cgcgcctcag ctgccggtag agcgcggcgg gcgagccgat
     3781 cagctgtcca tgaagcggaa acgtgaaatt gacctggtag cagtcacctt cttcgatgta
     3841 gcggcggata cgctcgatcg cgcgggtgta ggtcggtgcg tcgagggtcg ggcgcagggg
     3901 cgtgcaggcg gccggttctt catcggccag cgcggcgagg acggcatcgc aggcgtcgtc
     3961 gtccagccac tcggccctgg cgaagatcca ggcctggagc acgggggcgc caggggccgc
     4021 ggccggccga cgcagacggg gttcgatggt ctcgcccagt tcatacgtgg ctgcaagtgt
     4081 gacccaggcg ccggcgcggg cggcggcgtc gacacggtcg agtgcggccc gccagtcacc
     4141 gtcggcgcgg acgacgacct cctcgcgcag gtcggtgagc agccaggcgc cggacgtacc
     4201 gtcggcatcc aggttgttgt cgaaaagggc gaagggagga gaggggagga cggggggcac
     4261 ggcggcggag tttaccgcca gcgccggagg gatggacgca ttttcacgga cgggcgcctg
     4321 atgcggggtg ccgcagggac actcgcgtcc agacccgacg cgtgggtgtg taccggcggg
     4381 cgcagcgcgc agacgacggg atcctgccgc gcgtgcctgc ctgagtgcat cggatgatgt
     4441 caggccatgg cccacggcgc gccgggcacg cgtccgcaaa gtccgggaac gtccgcggca
     4501 ccctcgggtg tggtgccgcg gggccgctca gatcaacggt ccacccgagt gctcgccgcg
     4561 ttcgtatacg acgtgggcgc ggcccacgag gagcggatcg agggggccga tgtgctccag
     4621 atccttgttg gcatagggca gcttgtgaag gatgtagcgc atggcgttga ggcgcgcgcg
     4681 cttcttgcaa tcggacttga tgaccgtcca cggtgaatcg gccgtgtcgg tgtggaagaa
     4741 catggcttcc ttggctttcg tatagtcgtc ccacttgtcg agggaggcca ggtcgatggg
     4801 gctcagtttc cattgcttga gggggtggtt ttcccgctcc ttgaagcgcc ggcgctgctc
     4861 ttcgcgactc accgagaacc agaacttgac cagatgcacg ccgctgcggg caaggtggcg
     4921 ttcgaattcc ggcgtctggc gcatgaactc cagatactcg tcgtgggagc agaaacccat
     4981 cacccgctcc acgccggccc ggttgtacca ggagcggtcg aacatggtga tctcgccggc
     5041 agtggggagg tgttgaacat agcgctggaa gtaccactga ccacgttcgg tctcggaggg
     5101 cttttccagt gcgatcacgc gggcgccccg cggattgagg tgttccatga agcgcttgat
     5161 ggtgccgccc ttgcccgctg cgtcacggcc ttcaaagaga atcaccacgc gctggccggt
     5221 ctccttgacc caggcctgaa gcttgagcag ttctacctgg aggcggtatt tctgcttctc
     5281 gtaggccttg cgggacatga gattcttgta gggatagccc ccctcgcgcc atccctcggc
     5341 gagttcctcg tccgggtggg cggggagctt gctgtagatg acatcgtctt cgctctgcag
     5401 cgccttgcgc aggagcgcgg catcgtcggg cgaggcgccg gccaggatcg cctggagcgt
     5461 ctcggcgatg ctcgcggtgt cgtgcttggc gagggtggcc acgatgtcgc tgacggctgc
     5521 ggccttggag tcacgggccg cgtcgacggc ttcatcgacg gcgtaggctt caatgccttg
     5581 cgcattcagc gccggtgcgg tgtcgccctg cgctgcgcgg cgcgggcggg gcgccttgcc
     5641 attggaccgg ggggtggcgg gagccgcttt gtcgttcatg gccaaccttt ctgcttgtgg
     5701 cgacgtgatt cgggatggag cgcccgggcg catttctgaa cgcgtctcgg cgggcggggc
     5761 tgctgcatcc ggaattgtct gcgcggccgg tcatgacggc aatatatgac tttatgtcat
     5821 gacagaatgt cagaagtggg cgtcccagcg ggcccggtag gccttgcagc gttcgatgaa
     5881 gtccgtcgtg ctcttgggca tcggggtccg cgcacgggtg atgtagctga tcaggtcgcg
     5941 tccctgctgg atcaggcgac ggccgtcacc gaccagccgt tcggtgtccg gatcgttgtt
     6001 gggcaggacg tcgttgaaat gaccgcagag ccgttcggcg gccgtgacga aggttgtcca
     6061 gatgttgaag atgtccggat cggttcgcag ggtttccgtc ttgatcttgg ccgcattggc
     6121 gaattcaagc atgtgggcgg cgatgccatg gaaacagggc agcgggtcga aacattcgac
     6181 gacggtggcg gcgacacgat cgatgtcacg ctgggtcagg gcgatcttga tgtagcggct
     6241 ctcgtagaag tcctcgagcg ggatggaaaa ggccttgaac ggttcgcccc acagttcgtc
     6301 gatgccttcc tcgccgctga tgtgcaggac cagtcgcccc agacccaggg cttcctgcag
     6361 acacgcgcca cattcacgca tcagggcatt gtccgactgg atctcgatgc cgacgctttg
     6421 cagttcgaca agcttggtga agatgtccgc tgcccggttg aacaacgcgc cggcgagcat
     6481 cgcgcccttg acgcggcggc gtgccgggtc ggtctcggcc tcgaagctct ggcgggcctc
     6541 gttgagccgg ctgccgggta cattgagacc gtgctccacg tgattgaaca gacgccgtgt
     6601 cagcgcgccg atcaactggc gcttggcctg cagcgtgtcg gccgggacat ggtaggtctt
     6661 gatccagtac ccggagtaaa acacccgctc gcgaccgtcg atgatgcgct tggtgccttc
     6721 ggggatgtcg ctgtcggaga gatcggtagt catgttccgc tcttgttgga gaggtcgtgg
     6781 ggcggtcgtt catcgaggct cgaacgtttg attctgccct aagttttgat gagcgacgtg
     6841 ccacgacata caaattcccg ccggggtgtg gcgggcgtac gacagacttt ctggcagccc
     6901 tgcgcgtcag acgggcgtgt gggtgtgggt ccgcaggtct tccagcgtgt tgagattatc
     6961 gaaggccgcg ggacactcgt cgaaggcaca ggtcacggtg tgcagtccgt tgaaccaggt
     7021 ttcgacgcgc cgtccgccac cgtcgatgta ggccgtcagg gcctcagcgg tggtgcagcg
     7081 gatcagcgca aagaccggat gcaggcgccc gcccgcccgc gccacggcgg cgtcagcgcc
     7141 tcgggattcg agcgcgtcgg ccaggcgggc cacgagatcc tcggggaggc agggtgaatc
     7201 gcagggcgtg cacgccagga acggggtttc gcaggccgcc aggccggcgt cgagcccggc
     7261 gagcgggccg gcatggcctg ccaggcgatc ggcgatgacc ggatgaccga acgccgcgta
     7321 ggcatcggca ttgcgattgg cgttgatgag cagcgggccg acctggggtg caaagcgacc
     7381 gaggatgtgg gcgatcagcg gtttgccgtc caggtgcacg aggcccttgt cgcagccccc
     7441 catgcggcgt ccctgcccgc ccgccaggat gagtccggtg atgagggcga cgtccatggc
     7501 cctcagccgc cgatatagga catttcgatc ttgcgcaggt cggcggtggc ggcggtgcgc
     7561 agttcggagt agcgatcgtt ccggtcctgc cagattgcgc cgatggcggc agacagttcc
     7621 tcgtcgctgc agccttcccg cagcaggggc cgcaggtcgt ggccgctctg ggcgaacagg
     7681 caggtgtaca gacgcccctc ggtggacagg cgggcgcggg tacaggtgga acagaaggcc
     7741 tgggtcacgg atgaaatcac gccgatctcg ccgccgccat ccacgtaacg ccagcgctcg
     7801 gcgacctcac cggtatagtt ggggtcggtc gcctccagcg ggaactcggc cccgatgcgc
     7861 cggatgacct cggctgacgg caccacgtcg tccatgcgcc agccattgga gctgcccacg
     7921 tccatgaatt cgatgaaacg caggatgtgc ccgctgccgc gaaaatgccg ggccatgggc
     7981 aggatctcgc ggtcgttgag tccgcgcttg accaccatgt tgatcttgat ggggccaagc
     8041 ccggccgcgt cggccgcggc gatgcccgcc agcacatcgg cgaccggaac atccacgtcg
     8101 ttcatcgcgc ggaagatggc gtcgtccagg gcgtccaggc tcactgtcac ccgcgccagg
     8161 cctgcggcct tgagggcccg cgccttgcgc ggcagcagca caccgttggt ggtgagggtg
     8221 acctccatgt cgggcagcgc cgccagctgg gcgatcagat cctccagatt ccgtcgcagc
     8281 agcggttcgc cgccggtgag acggatcttg cgtacaccgt gggcggtgaa gaggcgtgcg
     8341 atacgggtga tttcctcgaa actcagcagc gcgttgcggg gcaggaaggc gtaatcctga
     8401 tcgaacaccg acttgggcat gcagtacacg caccggaaat tgcagcggtc ggtgaccgag
     8461 atgcgcagat cacgcacctg tcggccgcgc cgatcggcca gacgtccgtc cggagccggc
     8521 cggtcgctgg gaatggcggg gcgccttgca agctggcgct cgtcggcgag gggaatcact
     8581 ctctggttca tggggtcttc gcggtgggct ggcagaggtg ttcgatatac gcg
//
LOCUS       DAOQMM010000131         8503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_4175_length_8503_cov_52.543679, whole genome shotgun sequence.
ACCESSION   DAOQMM010000131 DAOQMM010000000
VERSION     DAOQMM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4175_length_8503_cov_52.543679"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1802
                     /gene="ilvD"
                     /locus_tag="PLH85_15615"
     CDS             3..1802
                     /gene="ilvD"
                     /locus_tag="PLH85_15615"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNB66047.1"
                     /translation="MAGARALWRATGMKDGDFEKPIIAVVNSFTQFVPGHVHLKDLGQ
                     LVAREIEAAGGVAKEFNTIAIDDGIAMGHGGMLYSLPSRDLIADSVEYMANAHTADAL
                     VCISNCDKITPGMLMAALRLNIPAIFVSGGPMEAGKVVWEAKVIPLDLVDAMVKAADS
                     HCSDEEVAAVERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGTVVATHADRERLF
                     KEAGRRIVDLARRYYEKDDESVLPRSIASFKAFENAISLDVAMGGSTNTVLHLLAAAK
                     EAGVDFTMQDIDRISRKVPCLSKVAPAVPDVHIEDVHRAGGIMAILGELNRAGLLHTD
                     VPTVHSKTLGEALTVWDVMQAHDAGVFQFYKAAPGGVPTQVAFSQDRRWNELDMDRSH
                     GVIRDKAHAFSQDGGLAVLYGNIAEKGCIVKTAGVDESIWQFTGRARVFESQEDAVAG
                     ILGDQIVAGDVVVIRYEGPKGGPGMQEMLYPTSYLKSKGLGKVCALLTDGRFSGGTSG
                     LSIGHASPEAACGGAIGLVEEGDTIEIDIPHRRIHLAVADDELARRRAAQDARGWKPA
                     NRVRHVSAALQAYAALTTSADTGAVRDVTQIQR"
     gene            1973..2965
                     /locus_tag="PLH85_15620"
     CDS             1973..2965
                     /locus_tag="PLH85_15620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB66048.1"
                     /translation="MPSSFLPASSGPLDTSSHTTPCQIEVEPNRRRWGALFARVPAPA
                     LTQSIAYGDAKAAVSGWEVERLVFHVGGWPVAICQVLVRRVWGLPVAARINRGPLFLA
                     AEPAVTEIRAVYGALRRRWRFGRRGVVFLAPGLADGALHRQCLQEAGFRPRSVAGWRS
                     ALLDLRQPAAELRRRLASNWRNHLNGAERNGLSFELERTPEALAWMLEHHADHMREKG
                     FAGTSVDFLQALARRAPDDVFVARVRQAGTPLAGMILCRFGNSAEYFVGWYGTEARRA
                     KAGHFLVWHAALAMAAVGCARFDLGGYSSSDGYGRFKQDMRGTEYTLADEWIAF"
     gene            complement(3017..3508)
                     /locus_tag="PLH85_15625"
     CDS             complement(3017..3508)
                     /locus_tag="PLH85_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB66049.1"
                     /translation="MNTVSIQPVDFGDAVRAEAYLDLLDHYMRGPTGSGTPLSPALRT
                     RLTAELRQRPTVLCFVAYLDDTPAGLLTCVEGFSTFAGRPLLNIHDIVVHERFRRRGL
                     ARALLEKAEDVARTRGCCKLTLEVLEGNAGARQAYLEFGFRGYELDPAMGRALFMEKR
                     LEG"
     gene            complement(3574..4530)
                     /locus_tag="PLH85_15630"
     CDS             complement(3574..4530)
                     /locus_tag="PLH85_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66050.1"
                     /translation="MTPVEAREWKTYSFQPQEAMDWKAAGFAPLVARTWSDKGFDPDE
                     ARAWVESTRQNRTLMAELDQTDPAAWKREGFSPADRLAWWEAGFVFEDAVLLSRAGMS
                     PAEAAWHGQEKLKELKEKSPTNVAGAAAPPVTGSPDSAPAAGLTLSLPDPARLWALTW
                     PYLKIAMASMVGMMVALIVFVYVRNRRLNRVHRSALEAAPDSELPPSPQPPAAAAAAE
                     SEGVNTTKSKHRRPARKFTLTRGQPTCVHCQSTHVRPSRMNPHRFLGVNFTEYYRCRN
                     CGRHFALVTYTPILLIAGSILLAVTMVTASVIYGLSLIPGSP"
     gene            4865..5161
                     /locus_tag="PLH85_15635"
     CDS             4865..5161
                     /locus_tag="PLH85_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB66051.1"
                     /translation="MSKWSRRAEEVRRLEDLPNVGPAIARDLRSIGIPDPAALRDADP
                     WALFACLNARDGVRHDPCVLDTFIAVTRFMNGDPARPWWAYTAERKRREAMDAG"
     gene            complement(5170..5595)
                     /locus_tag="PLH85_15640"
     CDS             complement(5170..5595)
                     /locus_tag="PLH85_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB66052.1"
                     /translation="MARVLIDLPEHFVFTTELPIYIGHINYGHHLDNAALLSLVSEAR
                     VRFFGSLGYTELDVEGVGIVVADAAVQYKSEAFHGETLVFAMTPAEFNKYGCDLVWRV
                     TDKASGREVARGKSGILFFDYQARRPVAVPDGFRIKTAP"
     gene            5848..6495
                     /locus_tag="PLH85_15645"
     CDS             5848..6495
                     /locus_tag="PLH85_15645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB66053.1"
                     /translation="MSVAAHLLVLAFGRGPQPELAPPRLLDARLVSESTPERPHEPPP
                     RHPPAETRPVHAAPAPHPQRQEALPVPSRMVSDAVSSRPTPAIATSDSPLPSRSEAPA
                     VAAPPAAAAVSSHAAYTPPSSSASYLDNPKPGYPMIARRRGLQGRVLLDVRVSAEGLA
                     LFVKVKESSGHDVLDEAAVNAVAHWRFAPARRGGEAVEGTASVPVQFRLNGTEPE"
     gene            6524..7141
                     /locus_tag="PLH85_15650"
     CDS             6524..7141
                     /locus_tag="PLH85_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNB66054.1"
                     /translation="MALSHWLAFFLTTLVVALSPGPGAVLSMSTGLRDGVRAALMAIA
                     GLQTALLVQLGLVALGMGAVLAASDTAFMVIKFAGAAYLILLGLRAWRDTGPPNVADR
                     GGAQRGSHYRQGILVNLGNPKAILFIAALVPQFVDPAQPRPPQFMIMALTLCTVDTVV
                     MSGYAMLAARCRAWMTDARAVRTRNRFFGTLFVVAGMALALATRA"
     gene            complement(7146..7958)
                     /locus_tag="PLH85_15655"
     CDS             complement(7146..7958)
                     /locus_tag="PLH85_15655"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNB66055.1"
                     /translation="MNGDEDALVRAVLASGVILPVPAPGFLRFQAAAADDTAGPKALA
                     AAVIDDPALTGALLRVASSPVFRPRSAPRNVLEAVSLLGRTRSLATVASTALRSRCDG
                     LDASAVDSLWRAGARAADAAFWACRASSCKSLADLTYLAALLQDVGTVVVLRRTPELA
                     GLLRCTEPDLEAGIHQLDTASGTCHAAAGYLVARNWKLPTAVCEALRLHHDLRATLRA
                     EPDVRHLGLLLAAGRRMRDGPDTPAWPEWSDPVAAELGLDSPTLDALAAGLG"
     gene            8065..8149
                     /locus_tag="PLH85_15660"
     tRNA            8065..8149
                     /locus_tag="PLH85_15660"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8099..8101,aa:Tyr,seq:gta)
     gene            8217..8290
                     /locus_tag="PLH85_15665"
     tRNA            8217..8290
                     /locus_tag="PLH85_15665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8249..8251,aa:Gly,seq:tcc)
     gene            8306..8380
                     /locus_tag="PLH85_15670"
     tRNA            8306..8380
                     /locus_tag="PLH85_15670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8338..8340,aa:Thr,seq:ggt)
BASE COUNT         1331 a         2711 c         3024 g         1437 t
ORIGIN      
        1 acatggccgg cgcccgcgcc ctgtggcgcg ccaccggcat gaaggacggc gattttgaaa
       61 agccgatcat cgccgtggtg aacagcttca cccagttcgt gccgggccac gtgcacctca
      121 aggatctggg ccagctggtg gcgcgggaga tcgaggcggc cggcggcgtc gccaaggagt
      181 tcaacaccat cgccatcgac gacggcatcg ccatgggcca cggtggcatg ctctacagcc
      241 tgcccagccg cgacctgatc gccgacagcg tcgagtacat ggccaatgcc cacaccgcag
      301 acgcgctcgt gtgcatctcc aactgcgaca agatcacccc gggcatgttg atggccgcgc
      361 tgcgcctcaa tattcccgcc atcttcgttt ccggcggccc gatggaagcc ggcaaggtgg
      421 tgtgggaggc caaggtgatc ccgctggatc tggtggacgc gatggtgaag gccgccgaca
      481 gccactgttc cgatgaggaa gtggccgccg tcgagcgttc cgcctgcccg acctgtggct
      541 cgtgttcggg catgttcacc gccaactcca tgaactgcct gaccgaagcc ctgggcctgt
      601 ccctgccggg caacggtacc gtggtggcca cccacgccga tcgggagcgc ctcttcaagg
      661 aagccggccg gcgcatcgtc gatctggccc gtcgctacta cgagaaggac gacgaatccg
      721 tcctgccccg cagcatcgcc agcttcaagg ccttcgagaa cgcgatcagc ctcgacgtcg
      781 ccatgggcgg ctccaccaac accgtgctgc acctgctggc cgcggccaag gaggccggcg
      841 tcgatttcac gatgcaggac atcgaccgca tctcccgcaa agtgccctgc ctgtccaagg
      901 tggccccggc ggtgcccgat gtgcacatcg aggatgtcca ccgcgccggc ggcatcatgg
      961 ccatccttgg cgaactcaac cgggccggcc tgctgcatac cgacgtgccc accgtgcata
     1021 gcaagaccct cggcgaggcg ctgaccgtgt gggacgtgat gcaggcgcac gatgcgggcg
     1081 tgttccagtt ctacaaggcg gcaccgggcg gcgtgccgac ccaggtggcc ttcagccagg
     1141 accgtcgctg gaacgagctg gacatggacc gcagccacgg tgtgatccgc gataaggccc
     1201 acgccttcag ccaggacggc ggcctcgccg tgctctacgg caacatcgcc gagaagggct
     1261 gcatcgtgaa gaccgccggc gtggatgagt ccatctggca attcaccggc cgcgcccgcg
     1321 tgttcgaaag ccaggaagac gccgtggccg gtatcctcgg cgaccagatc gttgccggcg
     1381 acgtggtagt gatccgctac gaagggccca agggcgggcc gggcatgcag gaaatgcttt
     1441 acccgaccag ctatctgaag tccaagggcc tcggcaaggt gtgcgcgctg ctcaccgacg
     1501 ggcgcttctc cggcggcacc tcgggcctgt ccattggcca cgcctcgccg gaagccgcct
     1561 gtggcggcgc catcggtctg gtggaggaag gcgacaccat cgagatcgac atccctcacc
     1621 ggcgcatcca cctggccgtg gccgacgacg aactggcccg ccggcgcgcc gcccaggatg
     1681 cccgtggctg gaagccggcc aaccgcgttc gccacgtgtc ggcggcgctg caggcttatg
     1741 cggcgctgac cacctcggca gacaccggcg cggtgcgtga cgtgacccag atccagcgct
     1801 gagccgcatc gctccctgtt gcgggggggt cttccggacc gctgcagacc gcgaattagt
     1861 acgcggtctg cagcggtttt tcattgtgca gacaagggct tgtgaccgat gtctggggag
     1921 tcgacggagg cggcggggag aatcggagcg ttccggactt tccccgaccg ccatgcccag
     1981 cagcttcttg ccggcttcat ccggcccgct cgacacctca tcacacacca ccccatgcca
     2041 gatcgaggtg gagcccaatc gccgccgctg gggtgccctg ttcgcccgcg tgcccgcacc
     2101 ggcgctcacc cagtccattg cttatgggga tgccaaggcg gccgtgagcg gatgggaggt
     2161 ggagcgcctg gtcttccacg tgggaggctg gccggtggcg atctgccagg tgctggtgcg
     2221 ccgtgtgtgg ggcctgccgg tggcggcgcg gatcaaccgc ggacccttgt tcctggcggc
     2281 ggagcccgcg gtgacggaga tccgcgccgt gtacggggcc ttgcgccggc gctggcgctt
     2341 cggccggcgc ggcgtggtgt ttctggcgcc agggctggcc gatggggcgc tgcatcggca
     2401 atgtctgcag gaggcgggct tccggccgcg gagtgtggcg ggatggcgct ctgcactcct
     2461 cgatctgcgc cagcctgcgg ccgaactgcg tcgccgcctg gcgtcgaact ggcgcaatca
     2521 tctgaacggg gcggagcgga acgggctgag tttcgaactg gagcggacac ccgaggcgct
     2581 ggcgtggatg ctcgaacatc acgcggacca catgcgggag aagggctttg cgggcacctc
     2641 ggtggatttc ctgcaggcgc ttgcccgtcg tgcgccggac gatgtcttcg tggcgcgggt
     2701 ccggcaggcg ggcacgccgt tggccgggat gatcctgtgc cgattcggga acagcgctga
     2761 gtacttcgta ggttggtacg gcacagaggc acggcgcgcg aaggcggggc acttcctcgt
     2821 ctggcacgcg gcgctggcca tggctgccgt gggatgcgct cgcttcgatc tgggcggcta
     2881 ttcgtcgtct gacggctatg gccgtttcaa gcaggacatg cgcgggacgg aatacacgct
     2941 ggcggacgaa tggatcgcct tttgattccg ggtcgtccgc ccgggtcggc ggcgcgcggg
     3001 gggcacgacc ggtgcttcag ccctccagcc gcttttccat gaacagggcg cggcccatcg
     3061 ccggatcgag ctcgtagccg cggaagccga attccagata cgcctgtcgc gcgccggcat
     3121 tgccttccag cacttcgagg gtgagcttgc agcaaccgcg tgtgcgggcg acgtcttcgg
     3181 ccttttccaa cagggcacgg gcgaggccgc gacgtcggaa acgttcgtgg acgacgatgt
     3241 cgtgaatgtt gagcagggga cgtccggcga aggtggaaaa tccttccacg caggtcagca
     3301 ggcccgccgg ggtgtcgtcc agataggcca cgaagcacag caccgtcggg cgctggcgca
     3361 gttcggccgt caggcgcgtc cgcagggccg ggctcagggg cgtgccactg ccggtcggcc
     3421 cgcgcatgta gtggtcgaga aggtccaggt aggcttcggc gcggacggcg tcgccgaagt
     3481 ccaccggctg aatgctgacg gtgttcatgg gtcacccgag acaggggaag ggcgcatctt
     3541 gccacgcccc gccctcggcc gggatgggct ccgtcagggc gagcctggaa tcagtgacag
     3601 cccgtagatg acggaggcgg tgaccatggt gaccgccagc aggatgctgc ccgcgatcag
     3661 caggatgggc gtgtaggtga cgagcgcgaa gtgccgtccg caattccggc accggtagta
     3721 ctcggtgaaa ttgacgccga ggaagcggtg gggattcatc cgcgacggcc gcacatgggt
     3781 gctctgacag tggacgcagg tgggctgccc gcgcgtgagc gtgaacttgc gggccggacg
     3841 acggtgtttg ctcttcgtcg tattgacgcc ttcggattcg gccgccgcgg cggcggcagg
     3901 gggctgcggc gagggcggca gctcgctgtc cggagccgcc tcgagtgcgc tacgatggac
     3961 cctgttgagg cgccggttgc ggacgtacac gaagacgatc agcgccacca tcatgccaac
     4021 catggaggcc atggcgatct tgaggtaagg ccaggtcagg gcccacagtc gggccggatc
     4081 gggaagactc agggtcagac cggctgcagg ggcgctgtcg ggcgagccgg tcacaggggg
     4141 cgcagcggcg ccggcgacgt tggtcgggct cttttccttc agttccttga gtttctcctg
     4201 accgtgccac gcggcttcgg cgggcgacat gcccgcccgt gacagcagga cggcatcctc
     4261 gaaaacgaag ccggcctccc accaggcgag gcggtcggct ggcgaaaacc cctcgcgctt
     4321 ccacgcggcg gggtcggtct gatcgagttc cgccatcagg gtgcggttct ggcgggtgga
     4381 ctcgacccag gcgcgcgcct cgtcggggtc gaagcccttg tcggaccagg tgcgggccac
     4441 caatggtgcg aatccggcgg ccttccagtc cattgcttcc tggggctgga acgaataggt
     4501 cttccactcc cgggcctcga caggcgtcat gcctgcttct tcccagcgtg tgacatgggg
     4561 ggcgctgggc gtcgcggcct ccgcggaggc tgcggccttt tgcgcagggg ccgtgggggc
     4621 gggtcgtgga ccagggtcgg caaccgcgat tccacagacg agaagcgcgg cggacaggaa
     4681 tgggagggtc tgtttcatgg tgcgaaacat taatgcactc cggggggcgc gcaccatgaa
     4741 gacttcgcgt gccccgtgaa ataaatcgta aggctatgcg aatatggccc tggcaagtgc
     4801 ggggcgcctc acttcgccac attcccgaaa taattcaagc ctgttgcgct gcgaaggggc
     4861 aaatatgagc aaatggtccc ggcgggccga ggaggtccgc cgtctggaag atcttcccaa
     4921 tgtcggtccg gcgatcgcgc gggacctgcg cagcatcggg atcccggatc cagcggcctt
     4981 gcgcgacgcc gacccgtggg cactctttgc gtgcctcaac gcccgcgatg gtgtccgcca
     5041 cgacccctgc gtactggaca ccttcatcgc ggtcacgcgc ttcatgaacg gcgacccggc
     5101 ccggccgtgg tgggcctaca cggccgaacg caagcgccgt gaggccatgg atgcaggctg
     5161 agcacggctt cagggggccg tcttgatccg gaagccgtcc ggcacggcga ccgggcggcg
     5221 ggcctggtag tcgaagaaga ggatgcccga cttgccgcgg gccacttccc ggccgctggc
     5281 cttgtccgta acgcgccaca ccaggtcgca gccgtatttg ttgaactccg ccggcgtcat
     5341 cgcgaacacc agggtttccc cgtggaaggc ctcggatttg tactggacgg cggcgtccgc
     5401 caccacgatg ccgacacctt ccacatcgag ttccgtgtag ccgagcgatc cgaagaagcg
     5461 cacccgggcc tcggagacga gggacaggag cgccgcgttg tccaggtgat ggccgtagtt
     5521 gatgtggccg atatagatgg gcagttcggt ggtgaagacg aagtgttccg ggaggtcgat
     5581 gagaacgcgg gccatgagtg atccttgctg tgggcgggtg cgacaaaagc gcgcagggtc
     5641 tgcggccttg atccagatcg tccaccgtgt cggagcgatc cggtaggatg cgcagtgtaa
     5701 gggggccggc ccagaggagt catgggccgg cagttttttt gtagcgaggg gcatgcccga
     5761 atgaatgccg gagccgcttg tctgtccggg cttgggaaaa gagaagtggg cgagacgccg
     5821 cagcgcggac tgtggttcgc actagggatg tccgtcgcgg cgcatctcct ggtcctggca
     5881 ttcggccgcg ggccgcagcc cgagctggcg ccgccacgcc tgctggacgc gcggctggtg
     5941 agcgagtcca cacccgagcg tccccatgag ccgccaccgc ggcatccgcc cgccgagacg
     6001 cgcccggtcc atgctgcgcc tgcgccgcat ccgcaacgtc aggaggcgct gccggtgccc
     6061 agccggatgg tctccgacgc cgtgtcgtcg cggccgacgc cggccatcgc gacgtccgac
     6121 tcgccgctac cgtcacgcag cgaagcaccg gccgtggctg cgccgccggc cgctgcggcc
     6181 gtgtcgtccc acgccgccta cacgccgccg agttcgtcgg cttcctatct ggacaatccc
     6241 aagcccggct atccgatgat cgcccgtcgc cgtggtctgc aggggcgcgt gctgctggac
     6301 gtgcgggtct ctgccgaggg cctggccctg ttcgtgaagg tgaaggagag ttccggccac
     6361 gacgtgctcg acgaggccgc ggtcaacgcc gtggcgcact ggcggtttgc gccggcacgg
     6421 cggggtggcg aggccgtgga gggcacggca agcgtgccgg tgcagttccg cctcaacggg
     6481 acggagccgg agtgaggcgc ccactacgcg ccgggcgcct gtgatggccc tgtcccattg
     6541 gcttgccttt ttcctgacga cactggtggt cgccctctcg ccggggccgg gtgctgtgct
     6601 gagcatgagt acaggcctgc gtgacggtgt gcgggcggcc ctgatggcca tcgccggtct
     6661 gcagaccgcg ctgcttgtgc aactgggcct ggtggcgctt ggcatggggg ccgtgctggc
     6721 tgcttcggac accgctttta tggtgatcaa attcgccggc gccgcgtacc tgatccttct
     6781 ggggctgcgt gcctggcgcg acacggggcc cccgaacgtc gccgatcggg gcggagcgca
     6841 gcggggcagc cattatcgcc agggaatcct ggtcaatctg ggcaacccga aggcgatcct
     6901 cttcatcgcg gcgctggtgc cccagttcgt cgatccggcg cagccgcgtc ctccccagtt
     6961 catgatcatg gcgctgacct tgtgcaccgt cgatacggtg gtgatgtccg gctacgccat
     7021 gctggcggcc aggtgtcgtg catggatgac cgacgcccgt gccgtgcgga cccgcaatcg
     7081 cttcttcggt acgctgtttg tcgtggcggg gatggccctg gcgcttgcga cccgggcctg
     7141 acccctcagc caaggcccgc cgcaagggcg tcaagcgtcg ggctgtcgag cccgagctcc
     7201 gctgcgaccg ggtcggacca ctccggccag gccggtgtgt ccgggccgtc gcgcatgcgt
     7261 cggccggcag ccagcaggag gccaaggtgg cgaacgtcgg gctcggcgcg cagcgtggcc
     7321 cgcaggtcgt ggtggagtcg gagggcctcg cagaccgctg tgggcagctt ccagttgcgg
     7381 gccacgagat agcccgccgc ggcatggcag gtgccgctgg ccgtgtcgag ctggtggatg
     7441 cctgcctcca ggtccggttc ggtgcagcgg agcagcccgg cgagctccgg tgtccgccgc
     7501 agcacgacga cggtgccgac gtcctgcagg agtgcggcga ggtaggtcag atcggcgagt
     7561 gacttgcacg atgacgcccg gcacgcccag aaggcggcat ccgctgcgcg tgcgccggcg
     7621 cgccagagcg agtcgacggc ggaagcgtcg agtccgtcgc agcgggacct gagggccgtg
     7681 ctggcgacgg tggccaggct gcgcgtgcgt cccagcagcg agacggcctc gagcacgttc
     7741 cgcggggcgc tgcgggggcg gaagaccggt gaactggcga cccgcaggag tgcgccggtg
     7801 agtgccgggt cgtcaatgac cgccgcagcc agtgcctttg gccccgcggt atcgtcggcc
     7861 gcagcggcct gaaaacgcag aaagccgggg gcggggaccg gcagtatcac gccgctggcg
     7921 agtacggcgc gaaccagtgc gtcctcatca ccgttcatgc gggggcgtct ccattctgag
     7981 gggactccat ggtgcccgat ttattttcct gaagagtgtt tgacattccc cgaaacggta
     8041 tctacaatgc ggggcttctc gcttggaggg gttcccgagc ggtcaaaggg atcagactgt
     8101 aaatctgacg gcactgcctt cgaaggttcg aatccttccc cctccaccaa caagctgtaa
     8161 tgctccgtcc gtgagttgcg gtaacgagag cggtgattgt gagtcgtgcg ttgcaagcgg
     8221 gtgtagctca atggtagagc agaagccttc caagcttatg acgagggttc gattcccttc
     8281 acccgctcca ggttgagtgt gtgaggccca tgtagctcag tggcagagca ctcccttggt
     8341 aagggagagg tcggcagttc aatcctgccc atgggcacca ctgattcccg gtgcggcggt
     8401 ttcgagtgtt gattccacta tttcctgatt gattgcgggg ttgatgaaat ggctaaggaa
     8461 aaatttgcgc ggacgaaacc gcacgtaaac gttggcacga tcg
//
LOCUS       DAOQMM010000132         8390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_4271_length_8390_cov_41.157169, whole genome shotgun sequence.
ACCESSION   DAOQMM010000132 DAOQMM010000000
VERSION     DAOQMM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_8390_cov_41.157169"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..639)
                     /locus_tag="PLH85_15675"
     CDS             complement(97..639)
                     /locus_tag="PLH85_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66056.1"
                     /translation="MNLRRALLLTALAHATLPLRALANPNAAAADTIGRDLRKLFLST
                     RAADLGLRPDSGTPPVFGAAFDWPVGTQFATIAALTDGSASLYTTGTYNILGGQAHAP
                     VRAAAQQFVQTAAQHLGSLAPLNNHPYPATNRARLFLLTFDGLRGIELTVDDLRPGSG
                     RYAQAFANAQTVLEALRKTV"
     gene            complement(838..1683)
                     /locus_tag="PLH85_15680"
     CDS             complement(838..1683)
                     /locus_tag="PLH85_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNB66057.1"
                     /translation="MTPTRRPFLKALAALGLAASLPARADEVLDAARSRGRLRIALYD
                     KFPPYSEGEKGIDAELGQALAARLGLTPQLIPFKADDDMADDLRNMVWKGHYLRGEPA
                     DVMLHVPVDRILADANDKVRIFGAYHLETIALAREPSRVPAPGGSAAVSLEVFTREKI
                     GVEGSTLADSFLLGVLRGRLRDNVVHFRSVREAVAALKDGQLAAVLAPRGELDAALGS
                     APGIVVEDARYPELSIKQWPVGMAVKADAPDLQAALTHALAELQADGTVAAIFRKHGV
                     RQVNP"
     gene            complement(1794..2318)
                     /locus_tag="PLH85_15685"
     CDS             complement(1794..2318)
                     /locus_tag="PLH85_15685"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB66058.1"
                     /translation="MTRSLPVLARSAVASLVLTAAHAALAAPALHVEESTTVAAPPAT
                     VWKLIGDYNALPAWHPAVARSDITKGTNNTRGAIRSLTTQDGAKIVEELLAYDGKARS
                     MTYRFLDTPMPITDYVATLAVMPEGHGSRIVWKSDFNRNASVDDAKAKGIITGIYQAG
                     LDALHKKLDAPAAH"
     gene            2621..4318
                     /locus_tag="PLH85_15690"
     CDS             2621..4318
                     /locus_tag="PLH85_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNB66059.1"
                     /translation="MGTESAGFRGKLWRGEGMCLIGMDVAAPEVDLVGFAIEVKSPGA
                     KTYTALRNRLNFAYDRPSVQAVTGARQFPSLLAPFQKFRWIHFPKDVQNGTYRYRLTK
                     IHMPAEGKLTKGTALELGIALDPETLPGFLNVAFTRNFASSQAFAERYGAQPALIPTK
                     ADDGLDFVKVAGDAYAWLGFEALALLNGTLDAALADPTCSVALLAYDLNEPDIVARLE
                     KLKGRLRAVIDNSKGHVEADSAESRAAARLAASAGAANVRRTRFKGLQHHKVIIVSRG
                     GTPVQVLFGSTNFSFRGLYIQANNMVSVTSPEVAGLFARMFDAAFADPDHFAAHPLAG
                     QWHEIRLAGRPPLSLCFSPHTDSDLSLGPVGQAIDGATSSVLFSIAFLNAIKSGPTRS
                     ALDRLAARPLFSYGVVDEDSGLALQKPDGSIGVVDFAYLAGKAPEPFRTEWSGGGGIN
                     IHHKFVVTDFNTPAASVFTGSSNLAPGGEAKNGDHLVRIQDPRVATAYAIEALRMFDH
                     LHFRVKMKAEQAAPRAPGALPPLVLQKPTALSGRPAWFEGAYRVGSQKARDRVLFSA"
     gene            complement(4447..4977)
                     /gene="lptA"
                     /locus_tag="PLH85_15695"
     CDS             complement(4447..4977)
                     /gene="lptA"
                     /locus_tag="PLH85_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNB66060.1"
                     /translation="MTSRIRPLLLALAALIATPFAHAEKADRSKPVNIEADKVTVDDK
                     NKIHIFEGHVILTQGTLTIRSDKLVVTQDMEGYQRGVATGGDGGLARFRQKRDNKDEW
                     VDGEGERLEHDARTELSQFFLRARVKSGQDEVRGQYIQYNGVTETYLVTNGPNATTLP
                     SQQSRVQVTLQPKKKD"
     gene            complement(4974..5555)
                     /gene="lptC"
                     /locus_tag="PLH85_15700"
     CDS             complement(4974..5555)
                     /gene="lptC"
                     /locus_tag="PLH85_15700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNB66061.1"
                     /translation="MKDRVHSLFPAIVLGLLAGASIWLDRITQQDQTLPQDNSRHEPD
                     FIVEQVKLRRYDVTGKTQYVLVADKMTHFADDESSDLTRPRLDYLNRPEPMHLESDRA
                     LVSKEGEKVLMSGNVFARRAATPDKPESTLRSDRMTVWPDDEKMLTDTPVTLTQGQTV
                     VTAERMDADNIAGELHLHGQVRGTFYRKQASKP"
     gene            complement(5587..6582)
                     /locus_tag="PLH85_15705"
     CDS             complement(5587..6582)
                     /locus_tag="PLH85_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNB66062.1"
                     /translation="MTPTPHPAARPQAVELARRVLRIEADAVAALAERLDAVAFTRAL
                     DLILASRGRVIVSGVGKSGHIGRKLAATLASTGTPAYFVHAAEAAHGDLGMITPDDVL
                     IAVSYSGAGDELLRIVPLVKRSGARLIALTGNPDSPLAREADVHLDGAVKEEACPHNL
                     APTASTTASLALGDALAVALLDARGFGPEDFARSHPGGTLGRRLLTRVSDIMTSGPGL
                     PRVTEDATLGDALLEMTRGGLGMTAIVDGADQVVGIYTDGDLRRSLAGGCDFASARVA
                     DVMTRNPRTIRAEALAVEAAEMMERLRISQLLVVDDARQLTGALNHIILMQAKVI"
     gene            6662..>8390
                     /locus_tag="PLH85_15710"
     CDS             6662..>8390
                     /locus_tag="PLH85_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB66063.1"
                     /translation="MVNTLELVLILLAGAVFVVGLFRSMNLPPVLGYLMVGAAVGPHA
                     MKLMPDTGGARYLAEFGVVFLMFSIGLEFSLPRLFAMKRIVFGLGAFQVLATMAFVTV
                     VGWLAGLGWGASFALGGTLAMSSTAILSKLLSDRLELDSKHGRETIGVLLFQDLAVVP
                     LLILIPALSGPAEALMLTLGLATAKAVGMLALVLFFGQKLMRGWFFHVARQRSPELFM
                     LNVLFITLGLAWMTESAGLSMALGAFLAGMLISETEFRYHVEEDIKPFRDVLLGLFFV
                     TVGMFLDVGEIVAHLAAVLAMLALLLVGKFVIVAAASRIMGSAQGTAVRTGLWLCAGG
                     EFGFVLLSEIADAGLLPPALMQVVVAALVLSMLLAPLIVQASEKIVLRLVPSEWMSRS
                     LQLTSIATQSMFVDKHAILCGFGRNGQYLGRLLEQEGITYIALDLDPERVREAAAAGE
                     TVVFGDAAKRETLVAAGIARASVVVVTYADTDAALRALSQVRSVRPDVPVVVRTADEV
                     DLEKLQEAGAAEVVPESVESSIMLASHALALLGVPLPRILKRIREIRAERYHLLRGIY
                     RGAHLHEEAV"
BASE COUNT         1126 a         2311 c         3461 g         1492 t
ORIGIN      
        1 ggggtggggc aggcgacggc atgccggaga gggtagcgga gtggcgcgga ttcggggggc
       61 tcgggcgggg cgtcgtccgt gaggctgcgc cgggtgtcag acggtctttc gcagggcttc
      121 gagaaccgtc tgggcgttgg cgaaggcttg agcgtatcga cccgaacccg gccggaggtc
      181 gtccaccgtg agttcaatgc cacgcagtcc gtcgaaggtg agaagaaaga ggcgggctcg
      241 attggtggcg ggataaggat ggttgttcag tggcgcaagg ctgcccagat gctgggctgc
      301 cgtttgtacg aactgttggg cggcggctct gaccggagca tgtgcctgac cgccgaggat
      361 attgtaggtg ccggtggtgt agaggcttgc ggagccgtcc gtgagggcgg caatcgtggc
      421 gaactgggtg ccgacaggcc agtcgaaggc cgcgccgaag acggggggcg tccctgagtc
      481 gggacgcagt ccgaggtcgg cggcgcgggt tgacaggaac agcttgcgca agtcacgccc
      541 gatggtgtcg gccgcggcgg catttgggtt ggcaagggcc ctgaggggta gtgtggcgtg
      601 agccagggcc gtgaggagga gagcgcgtcg tagattcatc atcgatacct gagcgggatg
      661 gtattggcat gatgatagcg ctacgggcgc tttgatgtag agattgccag tgacacgggg
      721 gcaggtgcgt ggggactcgg tgaggtgaac acccggcagt catttcctcg caggccggac
      781 ggccacactg gacccgatgc gggggtgggg atggcgggac ggggcgtctc ggcagcatca
      841 cggattgacc tgccgcaccc cgtgtttcct gaagatggcg gcaacagtgc cgtcggcctg
      901 gagttcggcg agggcgtggg tgagggcggc ttgcaggtcg ggggcgtcgg ccttgacggc
      961 catgccgacg ggccattgct tgatgctgag ttcggggtag cgggcgtctt cgacgacgat
     1021 gccgggggcg ctgccgaggg cggcgtcgag ttcgccgcgg ggggcgagga cggcggcgag
     1081 ctggccgtcc ttgagggcgg cgacggcttc gcggacgctg cggaagtgga cgacgttgtc
     1141 gcgcaggcgg ccgcgcagga cgccgagcag gaaggagtcg gcgagggtgc tgccttcgac
     1201 gccgatcttt tcgcgggtga agacttccag ggagacggcg gcggagccgc cgggggcggg
     1261 cacgcgggag ggttcgcggg cgagggcgat ggtttccagg tggtaggcgc cgaagatgcg
     1321 gaccttgtcg ttggcgtcgg cgaggatgcg gtccaccggg acgtgcagca tgacgtcggc
     1381 aggttcgccg cgcaggtagt ggcctttcca gaccatgttg cgcaggtcgt cggccatgtc
     1441 gtcgtcggcc ttgaagggga tgagctgggg cgtcaggccg aggcgggcgg cgagggcctg
     1501 gccgagctcg gcgtcgatgc ccttttcgcc ttcggagtag ggcgggaatt tgtcgtagag
     1561 ggcgatgcgc aggcggccgc ggctgcgggc ggcgtcgagg acctcgtcgg cgcgggcggg
     1621 caggctggcg gcaaggccga gggcggcgag ggccttgagg aaggggcggc gggtcggggt
     1681 catgacggtc tccggggggc gggcgaggga tggggcagga cgccggcgcc gctgcggccc
     1741 cggccggggg aggtcggccg gggcgggggc gatgcggcgc ggggccgtgt ggcttagtgt
     1801 gcggcgggcg cgtcgagttt cttgtgcagg gcgtcgaggc cggcctgata gatgccggtg
     1861 atgatgccct tggccttggc gtcgtccacg ctggcgttgc ggttgaagtc gctcttccag
     1921 acgatgcggc tgccgtggcc ttcgggcatg acggcgaggg tggcgacgta gtcggtgatg
     1981 ggcatggggg tgtcgaggaa gcggtaggtc atgctgcggg ccttgccgtc gtaggcgagg
     2041 agctcttcaa cgatcttggc gccgtcctgg gtggtgaggc tgcggatggc gccgcgggtg
     2101 ttgttggtgc ccttcgtgat gtcggagcgg gcgacggcgg ggtgccaggc gggcagggcg
     2161 ttgtagtcgc cgatgagctt ccacacggtg gccggcgggg cggcgacggt ggtgctctct
     2221 tccacgtgga gggccggggc cgcgagggcg gcgtgtgcgg cggtcaggac gaggctcgcc
     2281 acggcgctgc gggcgaggac ggggagggaa cgggtcacgg gaggtctcct tgttatgaat
     2341 gaatgggctg cggcagggcc tgagccgtga ccgattctag ggatggggcg gcggcgggga
     2401 agaggccgac ggcaggtcgg gcatggggcc gcgcgatgtc gggccttttg cccggcgaat
     2461 gcggtgtgga ccggtgcgat tgacgtgcgc cagccggtgc gaaaggctgc aggcggtgcg
     2521 cgaaggttct gcgccgctga ttccgatgtt ggcagcaggg tttttgcctt ctatgctgga
     2581 tgggaatggg tcgagggggc gggacatgga caatttttcg gtgggaactg aatccgcggg
     2641 ctttcgcggc aagctgtggc gcggcgaggg gatgtgcctg atcggcatgg acgtggcggc
     2701 gccggaggtg gatctggtgg gctttgccat cgaggtgaag tcgccggggg cgaagaccta
     2761 tacggccttg cgcaatcgcc tgaactttgc gtatgaccgg ccgtcggtcc aggcggtgac
     2821 gggggcccgg cagtttccgt cgctgctggc gccgttccag aaattccgct ggatccattt
     2881 tccgaaggac gttcagaacg ggacctaccg ttaccggctg accaagatcc acatgccggc
     2941 ggagggcaag ctgaccaagg ggacggcgct ggagctgggg attgcgctgg atccggagac
     3001 gctgccgggt tttctgaacg tggccttcac gcgcaacttt gcgtcgtccc aggcctttgc
     3061 ggagcgctac ggggcgcagc cggcgctgat cccgacgaag gcggacgatg ggctggattt
     3121 tgtgaaggtg gccggcgacg cctatgcgtg gctcggcttc gaggcgctgg cgctgctgaa
     3181 cgggacgctg gacgcggcgc tggccgatcc gacgtgttcg gtggcgctgc tggcctacga
     3241 tctgaacgag ccggacatcg tggcgaggct ggagaagctg aagggccggc tgcgggcggt
     3301 gatcgacaat tcaaagggtc atgtcgaggc ggacagcgcg gagagccggg cggctgcgcg
     3361 gctggcggcg tcggcggggg cggccaacgt gcggcggacc cgcttcaagg ggcttcagca
     3421 tcacaaggtg atcatcgtgt cgcgcggcgg cacgccggtg caggtgctgt tcgggtccac
     3481 gaacttcagt ttccgcggcc tctacatcca ggcgaacaat atggtgtcgg tgacgtcgcc
     3541 ggaggtggcg gggctgtttg cccgcatgtt cgacgcggcc ttcgccgatc cggaccactt
     3601 tgccgcccat ccgctggccg ggcagtggca cgagatccgg ctggcggggc gtccgcccct
     3661 gtccctgtgt ttctcgcccc acacggacag cgatctgtcc ctgggaccgg tggggcaggc
     3721 catcgacggc gcgacatcgt cggtgctgtt ctccattgcc ttcctcaacg cgatcaaaag
     3781 cgggccgacc cgctcggcgc tggatcggct ggcggcgcgg cccctgttca gctacggggt
     3841 ggtggatgag gattcggggc tggccctgca gaagccggac ggatccatcg gggtggtgga
     3901 ttttgcctat ctggcgggca aggcgccgga gcctttccgc acggagtggt cgggcggtgg
     3961 gggcatcaac atccaccaca agttcgtggt gaccgatttc aacacgccgg cggcgtcggt
     4021 atttaccggt tcgtccaatc tggcgccggg cggggaagcg aagaacggcg atcatctggt
     4081 acggatccag gatccgcggg tggcgacggc ctacgccatc gaggccctgc gcatgttcga
     4141 tcacctgcac tttcgcgtga agatgaaggc ggagcaggcc gcaccgcggg ccccgggcgc
     4201 tctgccgccg ctggtgctgc agaagccgac ggccctgagc ggccggccgg cgtggtttga
     4261 gggcgcctac cgggttggtt cgcagaaggc acgggaccgg gtgctgtttt cggcgtgaag
     4321 ggaaagatgg gggccgtcgc gctgcgtgga tagggcgtcg gcaggctaca aaaaaggagg
     4381 cgcgggcctc ccttttttgt gggtcgcagc tgctgcacgc cggggcggtg cggcgacgcc
     4441 gggcgttcag tccttcttct tcggctgcag ggtgacctgc acgcggctct gctggctggg
     4501 cagggtggtg gcgttggggc cgttggtgac gaggtaggtc tcggtgacgc cgttgtactg
     4561 gatgtactgg ccgcgcactt cgtcttgacc gctcttgacg cgggcgcgca ggaagaactg
     4621 ggagagttcg gtgcgggcgt cgtgttcgag gcgttcgcct tcgccgtcga cccattcgtc
     4681 cttgttgtcg cgcttctggc ggaagcgggc gaggccgccg tcgccgccgg tggcgacgcc
     4741 gcgctggtag ccctccatgt cctgggtgac gacgagcttg tcactgcgga tggtgagggt
     4801 gccctgggtg aggatgacgt gcccttcgaa gatgtggatc ttgttcttgt cgtccacggt
     4861 gaccttgtcg gcttcgatgt tgacgggctt ggagcggtcg gccttttcgg cgtgggcgaa
     4921 gggcgtcgcg atcagcgcgg cgagggcgag gaggaggggc cggatgcggg aagtcatggt
     4981 ttggaggcct gcttgcggta gaaggtgccc cggacctgac cgtggaggtg cagttcgccg
     5041 gcaatgttgt cggcgtccat gcgttcggcg gtgacgacgg tctgaccctg ggtcagggtg
     5101 acgggagtat cggtgagcat cttctcgtcg tcgggccaga cggtcatccg gtcggagcgc
     5161 agcgtggatt cgggcttgtc cggtgtggcg gcgcggcggg cgaagacgtt gccggacatg
     5221 agcaccttct cgccttcctt gctcaccagg gcgcggtcgg actcgagatg catgggctcg
     5281 ggccggttga ggtagtcgag gcgggggcgg gtgaggtccg aggattcgtc gtcggcgaag
     5341 tgggtcatct tgtcggcgac gaggacgtac tgggtcttgc cggtgacgtc gtaacggcgc
     5401 agcttgacct gctcgacgat gaagtcgggc tcgtggcggc tgttgtcctg gggcagggtc
     5461 tgatcctgct gggtgatacg gtcgagccag atgctggcgc cggccagcag gcccagcacg
     5521 atggcgggaa agagactgtg gacgcggtct ttcatgcggg cgcggacggg gcggggcggc
     5581 gcatcgtcag atgaccttgg cctgcatcag gatgatgtgg ttgagggcgc cggtaagctg
     5641 gcgggcgtcg tccaccacca gaagctggct gatgcgcagg cgctccatca tctcggcggc
     5701 ctcgacggcg agggcttcgg cgcggatggt gcgcgggttg cgggtcatga cgtcggccac
     5761 gcgggcgctg gcaaagtcgc agccgccggc gagggaacgg cgcaggtcgc cgtcggtgta
     5821 gatgccgacg acctgatcgg cgccgtccac gatggcggtc atgccgaggc cgccgcgggt
     5881 catttcgagg agcgcgtcgc cgagggtggc gtcttcggtc accctgggca ggccggggcc
     5941 ggaggtcatg atgtcggaga cgcgggtgag caggcgccgg ccgagggtgc cgccgggatg
     6001 ggagcgggcg aagtcttccg ggccgaagcc gcgggcgtcg aggagggcca ccgcgagggc
     6061 atcgccgagg gcgagggagg cggtggtgct ggcggtgggc gccaggttgt gggggcaggc
     6121 ctcttccttg acggcgccgt cgagatgcac gtcggcctcg cgggcgaggg gggaatcggg
     6181 gttgccggtg agggcgatga ggcgggcacc ggagcgcttg accaggggga cgatgcgcag
     6241 gagctcgtcg ccggcgccgg aataggagac ggcgatgagg acgtcgtccg gggtgatcat
     6301 gcccaggtcg ccgtgggcgg cttcggcggc gtgcacgaag taggccggcg tgccggtgct
     6361 ggcgagcgtg gcggcgagct tgcggccgat gtgaccggat ttgccgacgc cagagacgat
     6421 gacccggccg cgggaggcga ggatgaggtc gagggcgcgg gtgaaggcca ccgcatcgag
     6481 ccgctcggcg agggcggcca cggcatcggc ctcgatgcgc aggacccggc gggcgagttc
     6541 cacggcctgg ggccgcgcgg cgggatgcgg ggtcggtgtc atggacggcg tgggtataat
     6601 cgggcaacgg agtataacag aacggcccgg caccccttcc gggccccccg acgcctcacc
     6661 catggtcaat accctcgaac tggtcctgat cctgctcgcc ggcgcggtct tcgtggtcgg
     6721 cctgttccgc agcatgaacc tgccgccggt cctgggctat ctgatggtcg gcgccgcggt
     6781 ggggccccat gccatgaagc tcatgcccga cacgggcggc gcccgatatc tggcggagtt
     6841 cggcgtggtc ttcctgatgt tctccatcgg gctggagttc tcgctgccgc gcctgttcgc
     6901 gatgaagcgc atcgtgttcg gcctgggggc ctttcaggtg ctggcgacga tggctttcgt
     6961 cacggtggtg ggatggctcg ccggcctggg gtggggcgcg agctttgcgt tgggcggcac
     7021 gctggccatg tcgtccacgg cgatcctctc caagctgctg tcggatcgtc tggagctgga
     7081 ttccaagcac gggcgcgaga ccatcggcgt gctgctgttc caggatctgg cggtggtgcc
     7141 gctgctgatc ctgatccccg ccctgtcggg gcccgcggag gcgctgatgc tgacgctggg
     7201 actggcgacg gccaaggcgg tggggatgct ggcgctggtg ctgttcttcg ggcagaagct
     7261 catgcgcggg tggttcttcc atgtggcgcg gcagcgttcg ccggagctgt tcatgctcaa
     7321 cgtgctgttc atcaccctcg ggctggcgtg gatgacggag tcggcggggc tgtcgatggc
     7381 gctgggggcc ttcctggcgg gcatgctgat ctcggagacg gagttccgct accacgtgga
     7441 ggaggacatc aagcctttcc gcgacgtgct gctgggcctg ttcttcgtga cggtggggat
     7501 gttcctggac gtgggcgaga tcgtggccca cctggcggcg gtgctggcga tgctggcgct
     7561 cttgctggtg ggcaagttcg tgatcgtggc ggcggcctcg cgcatcatgg gcagtgccca
     7621 ggggacggcg gtgcgcacgg gcctgtggct gtgcgcgggg ggcgagttcg gcttcgtgct
     7681 gctgtcggag atcgcggatg cagggctgct gccgccggcc ctgatgcagg tggtggtggc
     7741 ggcgctggtg ctgtcgatgc tgctggcgcc gctgatcgtg caggccagcg agaagatcgt
     7801 gctgcgcctg gtgccgtcgg agtggatgag ccgctcgctg cagctgacga gcatcgcgac
     7861 ccagtcgatg ttcgtggaca agcacgcgat cctgtgtggc ttcgggcgta atggccagta
     7921 cctgggccgt ctgctggagc aggaggggat cacctacatc gcgctggacc tggacccgga
     7981 gcgggtgcgg gaggcggcgg cggccggcga gacggtggtc tttggcgatg cggcgaagcg
     8041 cgagaccctg gtggcggcgg gcatcgcgcg ggcctcggtg gtggtggtga cctacgcgga
     8101 tacggacgcg gccctgcgcg ccctgagcca ggtgcgttcg gtgcggccgg acgtgccggt
     8161 ggtggtgcgc acggccgacg aggtggatct ggagaagctg caggaggcgg gcgcggcgga
     8221 ggtggtgccg gaatcggtgg agtcgtcgat catgctggcc tcccacgcgc tggcgctgct
     8281 cggtgtgccc ctgccgcgca tcctcaagcg catccgcgaa atccgcgcag agcgctatca
     8341 cctgctgcgc ggcatctacc ggggtgccca cctgcacgaa gaggccgtgg
//
LOCUS       DAOQMM010000133         8176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_4474_length_8176_cov_50.760374, whole genome shotgun sequence.
ACCESSION   DAOQMM010000133 DAOQMM010000000
VERSION     DAOQMM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4474_length_8176_cov_50.760374"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..812)
                     /gene="rfbC"
                     /locus_tag="PLH85_15715"
     CDS             complement(258..812)
                     /gene="rfbC"
                     /locus_tag="PLH85_15715"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNB66064.1"
                     /translation="MNLIQTAIPDLLILEPKVFGDARGFFMESYNARTFRELTGLDVD
                     FVQDNHSRSAKGVLRGLHYQIQQAQGKLVRVVRGSVFDVAVDLRKASPTFGLWAGVEL
                     SEENQRQVWIPPGFAHGFLVTSDSADFLYKTTDYYAPQHERCLLWNDPVVGVAWPLAQ
                     IDGAPQLSAKDVAGKGLADCEVFA"
     gene            complement(825..1706)
                     /gene="rfbA"
                     /locus_tag="PLH85_15720"
     CDS             complement(825..1706)
                     /gene="rfbA"
                     /locus_tag="PLH85_15720"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNB66065.1"
                     /translation="MQRKGIILAGGSGTRLHPATLAVSKQLIPVYDKPMIYYPLSTLM
                     LAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYAVQPSPDGLAQAFIIGADFVGNA
                     PSALVLGDNLFYGHDFSSNLRTASARTDGATVFAYPVHDPERYGVVEFDAQGHAVSLE
                     EKPRQPKSRYAVTGLYFYDQRVVDVARAIQPSPRGELEITDVNRQYLEWGALDVQVMG
                     RGHAWLDTGTHESLLEASLFIQTLEKRQGLKIACPEEIAWRAGWIDAARLQQLAAPLA
                     KNGYGQYLLRILDEKVF"
     gene            complement(1718..2623)
                     /gene="rfbD"
                     /locus_tag="PLH85_15725"
     CDS             complement(1718..2623)
                     /gene="rfbD"
                     /locus_tag="PLH85_15725"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNB66066.1"
                     /translation="MKILLLGKNGQVGWELQRSLAPLGDLVALDRHGDGGWIGDLADP
                     DGLAATVRAVAPDLIVNAAAHTAVDKAESEPDLAFTINALAPGALARAAATCGALLVH
                     YSTDYVFDGRGTAPWAEGAPTGPLSVYGRTKLEGETLIRDSGCRHLIFRTSWVYAARG
                     GNFARTMLRLAVERDALSVVDDQHGAPTGAELLADVTAHAARMTFARPELAGTYHLAA
                     AGETTWHGYARHVVEFARARGVPIRVAPDAIRPVPTSAFPTPAQRPANSRLDTRKLRD
                     AFGLVLPDWQQGVERMLTEILPNSN"
     gene            complement(2683..3741)
                     /gene="rfbB"
                     /locus_tag="PLH85_15730"
     CDS             complement(2683..3741)
                     /gene="rfbB"
                     /locus_tag="PLH85_15730"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNB66067.1"
                     /translation="MILVTGGAGFIGANFVLDWLATCDEPVVNLDALTYAGNLENLAS
                     LKGDPRHVFVQGDICDRPLIDRLLAEHQPRAIVHFAAESHVDRSIHGPGEFMRTNVTG
                     TFTLLEAARAHWNGLEGAAKADFRFHHVSTDEVYGSLGPTDPAFTETKTYEPNSPYSA
                     SKAASDHLVRAWHHTYGLPVTTSNCSNNYGPYHFPEKLIPLMIANALAGKPLPVYGDG
                     MQIRDWLYVTDHCSAIRAILEKGQPGEVYNVGGWNEKPNIEIVRTLCALLDELKPDPA
                     GSYARLITYVTDRPGHDRRYAIDARKIERELGWRPAETFDTGIRKTVEWYLAHQDWVA
                     HVQSGAYRDWVEQNYAAR"
     gene            3968..5932
                     /locus_tag="PLH85_15735"
     CDS             3968..5932
                     /locus_tag="PLH85_15735"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNB66068.1"
                     /translation="MPFSAPRPFSWKQASVVVPTLIALLLALVGVSQGLHRLTQEKEQ
                     DQALDFAYRSAEMHFQIGERFKAYRQVLRGARALFAASERVSRSEWKAYARELRLDTE
                     FPGIQGVGFALSVAAAHQGAHEQAVREEGFADYRISPPGERAEYAPIVYLEPFDWRNR
                     RAFGYDMLSEPVRRTAMLRTRSLGLATLSGKVRLVQETETDPQAGVLLYLPVFRNGTS
                     PATPAQREEAFVGWVYSPIRMKDLITGTLGNTPDRIRLRIFDGESRTAEALLFDSHPE
                     LAQVGRFQATYVLELDGRTWTLAFESTPAFSMGKTVDTVEATSVVLIGALIVLLTASF
                     AGARQRALDLDRIGASLRASEARYSTLVNLSRDGIAAVDGGLRFTFVNPRLAELLGHP
                     ADALLGRRLDSLWPAPDEPRSRTLIRRLHRGEGATYEQELIGARGAAVTTQISDAPLL
                     GRNGALLSVIVTVTDISERKESEQRIRYLATHDALTGLANRTMFLELMDASLRLTSRR
                     GTRLAVFFLDLDGFKAVNDRLGHHAGDQLLVATARRIRACLRASDHLARQGGDEFLAL
                     IHDVAGADEAVAVARKIHQSLEEPFDLPEGRALISVSIGIALFPVDGTDIDTLTRQAD
                     SAMYQAKAGGRNGIRLAGEPVPPEAPQQGS"
     gene            5995..7437
                     /gene="pyk"
                     /locus_tag="PLH85_15740"
     CDS             5995..7437
                     /gene="pyk"
                     /locus_tag="PLH85_15740"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNB66069.1"
                     /translation="MSRHTKIVATLGPASSDPATLERLVQTGVDVVRLNFSHGTAEDH
                     IRRATQIRDAAQLVGRPVGILADMQGPKIRIGKFEQGKIALDKDAPFILDARCELGNA
                     QRVGLDYKDLPRDVKPGDVLLLDDGRLKLGVERVLGHEIHTRVKVGGELSNNKGINRQ
                     GGGLTAPALTAKDMEDIKTAAQIGVDFVAISFPKGAADMYMARQLLRAAGSSAVLIAK
                     IERTEAVANLEEILDASDGIMVARGDLAVEVGDAAVPALQKRMIRLARERNKLAITAT
                     QMMESMIHSPVPTRAEVSDVANAVLDGTDAVMLSAETASGKFPVEVVEAMARICLEAE
                     KSAEVTMDRDFLDRIFTRIDQSIAMAAMWTAYHLKVKAIASLTQTGSTALWMSRLNSG
                     VPIYALTPELTSRNQMTLYREVYPLLMSQMHADRDMLLWESEQILLEQGVVDYGDLIV
                     LTIGEPIGASGGTNTMKIVRVGDTPRPGPV"
     gene            7552..>8176
                     /locus_tag="PLH85_15745"
     CDS             7552..>8176
                     /locus_tag="PLH85_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HNB66070.1"
                     /translation="MPLVSMRQLLDHAAENGYGLPAFNVNNLEQVQAIMEAAHETDSP
                     VIMQASAGARKYAGEAFLRHLIDAAVEAYPHIPVVMHQDHGQSPAICMGAIRSGFSSV
                     MMDGSLLEDGKTPSSYEYNVDVTRRVVEMSHAIGVSVEGELGCLGSLETGQAGEEDGV
                     GAEGTLDHSQMLTDPDQAADFVRQTNVDALAIAIGTSHGAYKFTRKPT"
BASE COUNT         1326 a         2780 c         2768 g         1302 t
ORIGIN      
        1 agaagacgtc gctcatctcc cggtcgtagt gctggccggt gtggatgatc ttgaaggtga
       61 aacggtcggc ccgcgcctgc aacgcccgca cgatgggcgc gatcttcatg aagttgggac
      121 gcgcaccggc gacgaggtag atcagggact gggacatgcg gcactccggg cgaactaatg
      181 acaggcgcgc attatcgcgc gctgatcatg accggcacaa agaaatagtt gtcagactct
      241 gttcccgctc cgcccggtca ggcgaagacc tcgcagtcgg ccaggccctt gccggccaca
      301 tccttggcag agagctgcgg cgcgccgtcg atctgcgcga ggggccacgc cacccccacg
      361 accggatcgt tccagagcag gcagcgttcg tgctgcgggg cgtagtaatc ggtggtcttg
      421 tagaggaagt cggcgctgtc gctggtgacc aggaaaccgt gggcaaaacc cggcgggatc
      481 cacacctggc gctggttttc ttccgacagc tcgacccccg cccacagccc gaaggtgggc
      541 gaggccttgc gcaggtctac cgccacgtcg aagacgctgc cgcgcaccac gcgcaccagc
      601 ttgccctggg cctgctggat ctggtagtgc aggccgcgca gcacgccctt ggcggagcgg
      661 gagtggttgt cctgcacgaa gtccacgtcg agaccggtga gctcgcgaaa ggtccgggcg
      721 ttgtagctct ccatgaagaa gccacgggcg tcgccgaaca ccttgggttc gaggatcagc
      781 agatcgggga tggccgtttg gatgaggttc atcggaccct ccggtcagaa gactttttcg
      841 tcgaggatgc gcaggaggta ctgcccgtag ccattcttgg cgaggggcgc ggcgagttgc
      901 tggagtctgg ccgcgtcgat ccagccggcg cgccaggcga tctcttcggg gcaggcgatc
      961 ttgaggccct ggcgcttctc aagggtctgg atgaacaggc tggcttcgag caggctctcg
     1021 tgggtgccgg tgtcgagcca ggcgtggccg cggcccatga cctgcacgtc gagggccccc
     1081 cattccagat attggcggtt cacgtcggtg atctccagct cgccgcgggg gctgggctgg
     1141 atggcgcggg ccacatccac gacgcgctgg tcgtagaagt agaggccggt gacggcgtag
     1201 cggctcttgg gctgcctggg cttttcttcg agactgacgg cgtggccctg ggcgtcaaat
     1261 tcgacgacgc cgtaacgctc ggggtcatgc accggatagg cgaagacggt ggcgccgtcg
     1321 gtccgggcgc tggcggtgcg cagattgctg ctgaagtcgt ggccgtagaa caggttgtca
     1381 ccgagcacca gggcgctggg ggcattgccg acgaagtctg cgccgatgat gaaggcctgg
     1441 gcgaggccgt ccgggctggg ctggacggcg tactggaggt tgaggcccca ctggctgccg
     1501 tcgcccagca gttgctggaa gcgcggagtg tcctggggcg tggagatgat caggatgtcg
     1561 cggatgcccg ccagcatgag ggtgctgagg gggtagtaga tcatcggctt gtcgtacacc
     1621 gggatgagct gcttggagac ggccagggtg gccgggtgca gccgggtgcc ggagccgccg
     1681 gcgaggatga tgcctttgcg ttgcatggtg gtctttctca gttcgagttc ggcaggattt
     1741 cggtgagcat gcgttcgacg ccctgttgcc agtcaggcag gacgaggccg aaggcatcgc
     1801 gcagcttgcg ggtgtcgagg cgcgagttgg ccgggcgctg ggcgggggtc gggaaggcgc
     1861 tcgtgggcac cgggcggatc gcgtcgggtg caacccggat cggcacgccg cgggcgcggg
     1921 cgaattcgac gacgtggcgg gcgtagccat gccaggtggt ctcgcccgcg gccgccaggt
     1981 gataggtgcc ggccagttcg gggcgggcga aggtcatgcg cgcggcgtgg gcggtgacgt
     2041 cggcaaggag ctcggcgccg gtgggggcgc catgctggtc gtcaacgacg ctcagggcgt
     2101 cccgctcgac ggccagacgc agcatggtcc tggcgaagtt gccgccgcgg gcggcataca
     2161 cccagctggt acggaagatg aggtggcggc agccgctgtc gcggatgagg gtttcccctt
     2221 cgagcttggt ccggccgtag acggagaggg ggccggtggg ggcgccttcg gcccacggcg
     2281 cggtgccgcg gccgtcgaag acgtaatcgg tggagtagtg gacgagaagc gccccgcagg
     2341 tggccgccgc ccgtgccagc gcgccgggcg cgagggcgtt gatcgtgaag gccagatccg
     2401 gctcgctttc ggccttgtcc accgccgtgt gggcggcggc attgacgatg aggtccggcg
     2461 ccacggcccg cacggtggcg gcgaggccgt ccggatcggc cagatcgccg atccacccgc
     2521 catcgccgtg acgatccagg gcgacgaggt caccgagggg ggcgagactg cgctggagct
     2581 cccagccgac ctggccgttc ttgccgagga gcaggatctt catgggcatc accgctatcc
     2641 gtggtggaaa aactgacgtc cgggccgggg ctcgcccagc gctcagcgtg ccgcgtagtt
     2701 ctgctcgacc cagtcccggt aggcgccgct ctgcacgtgg gcgacccagt cctgatgcgc
     2761 cagataccac tcgacggtct tgcggatgcc ggtatcgaag gtctcggcgg ggcgccagcc
     2821 cagctcgcgc tcgatcttgc gggcgtcgat ggcgtagcgg cggtcgtggc cgggacggtc
     2881 ggtgacgtag gtgatcaggc gtgcgtagga accggccggg tcgggcttga gttcgtcgag
     2941 gagggcgcac agggtgcgca cgatctcgat gttgggcttc tcgttccagc cgcccacgtt
     3001 atacacctcg ccaggttgcc ctttttcgag gatggcgcgg atcgcggagc agtggtcggt
     3061 gacgtacagc cagtcgcgga tctgcatgcc gtcgccgtac acgggcaggg gcttgccggc
     3121 cagggcgttg gcgatcatca gcgggatgag cttttccggg aagtggtagg gcccgtagtt
     3181 gttggagcag ttgctggtgg tgacgggcag gccgtaggtg tgatgccagg cgcgcacgag
     3241 gtggtcggag gcggccttgc tggcggagta ggggctgttg ggttcgtagg tcttggtctc
     3301 ggtgaaggcc gggtccgtgg gaccgaggga gccgtagacc tcgtcggtgc tcacgtggtg
     3361 gaagcggaag tcggccttgg cggcgccttc gaggccgttc cagtgggcgc gggccgcttc
     3421 gagcagggtg aaggtgccgg tgacgttggt gcgcatgaat tcgccggggc cgtggatgct
     3481 gcggtccacg tggctttcgg cggcgaaatg gacgatcgca cggggctggt gttcggccag
     3541 caggcggtcg atgagggggc ggtcgcagat gtcgccctgc acgaagacgt gacgggggtc
     3601 gcccttcagg gaggcgaggt tctcaaggtt gcccgcgtag gtgagggcgt cgagattgac
     3661 caccggctcg tcgcacgtgg cgagccagtc cagaacgaag ttggcaccga tgaaaccggc
     3721 gccgccggtg acgagaatca tgcaggatcc ttgcgagtca gaaataacgg aaagcgggca
     3781 gtccacccca tccaggcggg cgccagcccg tgattatgac accgccgccg ctctgcctga
     3841 cgggcttttc cggactcgcc cacttctctc ctcaatgtcg gcccgatcgt ggccgtagac
     3901 cggaggaaca ccgcccgccg caccggaacc ggggccccgt gcgcgcaccg tccaggagtc
     3961 gatcaggatg cccttttccg ccccccgccc cttcagctgg aagcaggcca gcgtcgtcgt
     4021 cccgacgctg atcgcgctgc tgctcgcgct tgtgggggtg tctcagggac tccaccgcct
     4081 gacccaggag aaggagcagg atcaagccct cgatttcgcc taccgctctg cggagatgca
     4141 tttccagatc ggcgagcgct tcaaggccta tcgccaggtg ctacgcggcg cgcgggcgct
     4201 gtttgcggcc tctgaacgag tgtcccgcag cgaatggaag gcctatgccc gcgaactgcg
     4261 tctggatacg gaatttccgg gcattcaagg ggtcggcttc gccctgtcgg tcgctgcggc
     4321 acatcagggc gcccacgaac aggcggtccg tgaagagggg tttgccgact accgcatcag
     4381 cccgccgggt gagcgcgcgg agtacgcgcc catcgtctat ctcgaaccct tcgactggcg
     4441 caaccgccgc gccttcggct acgacatgct gtccgagccg gtgcgccgca ccgccatgct
     4501 acggacccgc agcctcggtc tcgccaccct gtcgggcaag gtccggctgg tgcaggagac
     4561 cgagaccgac ccgcaggccg gcgtgctcct gtacctgccg gtcttccgca acggcacctc
     4621 gcccgccacc ccggcccagc gggaagaggc cttcgtcggc tgggtgtaca gcccgatccg
     4681 gatgaaggat ctgatcaccg gcaccctcgg caacacgccg gaccggatcc ggctgcgcat
     4741 cttcgatggc gaatcccgga cggcggaggc cctgctcttc gacagccacc cggaactcgc
     4801 acaggtgggg cgctttcagg ccacctacgt gctggaactg gatggtcgca cctggaccct
     4861 cgccttcgag agcacccccg ccttcagcat gggaaagacc gtggatacgg tggaagccac
     4921 gtccgtggtc ctgatcggcg cgctcatcgt gctgctcacc gcctccttcg cgggcgctcg
     4981 ccagcgcgcc ctggacctgg accgcatcgg cgcctccctg cgtgccagcg aagcccgcta
     5041 cagcaccctg gtcaatctct cgcgggacgg catcgccgcc gtcgacggcg gcctgcgctt
     5101 caccttcgtg aacccccgac tcgccgagct gctcggtcac ccggccgacg ccctgctcgg
     5161 acgccgtctc gacagcctgt ggccagcgcc cgacgagccc cgcagccgga cgctcatccg
     5221 ccgcctgcac cggggcgaag gcgcaaccta cgagcaggag ctgatcggcg cccgcggcgc
     5281 ggccgtcacg acccagatca gcgacgcacc gctgctcggc cgcaatggtg cgctcctcag
     5341 cgtcatcgtc accgtcacgg acatctccga acgcaaggaa tcggagcagc gcatccgcta
     5401 tctggcgacc catgacgcgc tcaccggact ggccaatcgc acgatgttcc tggaactcat
     5461 ggacgccagc ctgcgcctga cgagccggcg cggcacgcgt ctggcggtct tcttcctgga
     5521 cctggacggc ttcaaggccg tgaatgaccg cctcggccac cacgctggcg accagctgct
     5581 ggtcgccacc gcccgccgca tccgcgcctg cctgcgggcc agcgatcacc tggcccgcca
     5641 gggcggggac gaattcctgg cgctgatcca cgacgtggcc ggcgccgacg aggccgtcgc
     5701 ggtcgcccgc aagatccacc agagcctcga agagcccttc gacctgccgg aaggccgcgc
     5761 cctgatctcg gtcagcatcg gcatcgccct ctttccggtg gatggcaccg acatcgacac
     5821 cctcacccgc caggccgact cggccatgta ccaggccaag gccggcggtc gcaacggcat
     5881 ccgcctggct ggcgagcccg tcccgcccga ggcgccgcag cagggttcat gattcgcgcc
     5941 tagaatcacg tccgcatcga ccacaaccat aatctgtcat cgcctggaga cgccatgtcc
     6001 cgtcacacca agatcgtcgc caccctgggg cccgcctcgt ccgatcccgc caccctcgaa
     6061 cgcctcgtcc agaccggggt ggacgtggtg cgcctgaatt tctcccacgg caccgccgaa
     6121 gaccacatcc gccgcgcgac ccagatccgc gacgcggccc agctggtcgg tcgtccggtg
     6181 ggcatcctcg ccgacatgca gggccccaag atccgcatcg gcaagttcga acagggcaag
     6241 atcgccctcg acaaggacgc ccccttcatc ctcgacgcaa gatgcgagct gggcaacgcc
     6301 caacgcgtcg gcctcgacta caaggacctg ccccgcgacg tgaagccggg cgacgtgctg
     6361 ctgctggacg acggccgcct caagctgggc gtcgagcgcg tcctcggcca cgagatccac
     6421 acccgcgtga aggtgggcgg cgagctgtcc aacaacaagg gcatcaaccg ccagggcggc
     6481 ggcctcaccg cgccggccct caccgccaag gacatggagg acatcaagac cgccgcccag
     6541 atcggcgtcg acttcgtcgc catctccttc cccaagggcg ctgccgacat gtacatggcg
     6601 cgccagttgc tgcgcgccgc cggcagcagc gccgtgctca tcgccaagat cgaacgcacc
     6661 gaggccgtgg ccaacctgga agagatcctc gacgcctccg acggcatcat ggtggcccgc
     6721 ggcgacctgg ccgtggaagt gggcgacgcc gccgtgccgg ccctgcagaa gcgcatgatc
     6781 cggctcgccc gcgaacgcaa caagctggcc atcaccgcca cccagatgat ggaatccatg
     6841 atccacagcc cggtaccgac ccgcgccgag gtctcggacg tggccaacgc ggtgctcgac
     6901 ggcaccgacg cggtgatgct ctccgccgag actgcctctg gcaagttccc ggtcgaggtg
     6961 gtcgaggcca tggcacgcat ctgcctggaa gccgaaaaat ccgccgaggt caccatggac
     7021 cgggacttcc tcgaccggat ctttacccgc atcgaccaat ccatcgccat ggccgccatg
     7081 tggaccgcct accacctcaa ggtcaaggcc atcgcctccc tcacccagac cggctccacg
     7141 gccctgtgga tgagccgcct caacagcggc gtgcccatct acgccctcac cccggagctc
     7201 acctcacgca accagatgac cctctaccgg gaggtctacc cgctgctcat gtcccagatg
     7261 cacgccgacc gggacatgct cctgtgggaa tccgaacaga tcctgctgga gcagggcgtc
     7321 gtcgattacg gcgacctcat cgtgctcacc atcggcgaac ccatcggtgc ctccggcggc
     7381 accaacacca tgaagatcgt gcgcgtcggc gacaccccca gaccgggtcc cgtgtaatcc
     7441 atcacgctcg cggccccgcg ccggcccgtc cacgaggcat gccggcgcga tagaattccg
     7501 cttttccctt gcaccctggc cggcccctgc cggtcctccg gagatttcac catgcctctc
     7561 gtttccatgc gtcagctgct cgaccacgcc gccgaaaacg gctacggcct gcccgcgttc
     7621 aacgtcaaca acctcgaaca ggtccaggcc atcatggaag cggcccatga aaccgacagc
     7681 ccggtgatca tgcaggccag cgccggcgcc cgcaaatacg ccggcgaagc cttcctgcgc
     7741 cacctcatcg acgccgccgt cgaagcctat ccgcacatcc ccgtggtgat gcaccaggac
     7801 cacggccagt cccccgccat ctgcatgggc gccatccgct ccggcttctc gtcggtgatg
     7861 atggacggct ccctgctgga agacggcaag accccgtcct cctatgaata caacgtggat
     7921 gtcacccgcc gcgtcgtcga gatgtcccac gccatcggcg tcagcgtcga aggcgagctg
     7981 ggctgcctgg gctccctcga aaccggccag gccggcgagg aagacggcgt cggcgccgaa
     8041 ggcaccctgg atcattccca gatgctcacc gaccccgacc aggccgccga cttcgtccgc
     8101 cagaccaacg tggacgccct cgccatcgcc atcggcacca gccacggcgc ctacaagttc
     8161 acccgcaagc ccaccg
//
LOCUS       DAOQMM010000134         7840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_4783_length_7840_cov_51.163776, whole genome shotgun sequence.
ACCESSION   DAOQMM010000134 DAOQMM010000000
VERSION     DAOQMM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4783_length_7840_cov_51.163776"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1114)
                     /locus_tag="PLH85_15750"
     CDS             complement(116..1114)
                     /locus_tag="PLH85_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB66071.1"
                     /translation="MLTADARDFHLLNDFQRGFPLRAEPWAALAAHLGCGADEVMQRL
                     ARLRDSGKISRVGPVFAPNRLGASTLAAMAVPPAELPRVAALVSAFAGVNHNYRREHR
                     FNLWFVAAAADERALNGLLAAIGSATGCPPVALPLVEEFHIDLGFDLANGRRTRHKPA
                     PVGMKRALSAAEWRLVEALQDGLPWVPRPYRAVADAAGLNEEDVLATLAAWLAEGTLR
                     RFGVVVRHRELGWDANAMCVWAVPEAAVSAAGRALAAQDGVSLCYRRRTAPEWPYSLF
                     CMLHGRSRAEVEARRMTVADAAGVAGYPSAVLFSTDCYKQCGARYRDATPQPACVS"
     gene            complement(1118..>1903)
                     /locus_tag="PLH85_15755"
     CDS             complement(1118..>1903)
                     /locus_tag="PLH85_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNB66072.1"
                     /translation="DADTLELLADVPAEVAPGRFSRVVGLADAPGRRFVYALFDGDEI
                     RVSDFSDPRHPVTTRYPAGRQPYDGLVTPDGRYFLAGLFGEDGVSLLDLWQPEKGVTR
                     ILAGYGRGEEKLPVFKMPHLRGWSLAAGQAWLPAVGRHEVLAVDTRTWAETARIPVKG
                     QPVFVMARPDGRQIWVNFAFPDNDWVQVIDTVERRVIHSFSPGKAVLHLEFTPRGEEV
                     WISSRDSDRVSVVDTATFATRTELPAAAPSGIFFTARGQRIGF"
     assembly_gap    1904..2003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2150..2494)
                     /locus_tag="PLH85_15760"
     CDS             complement(2150..2494)
                     /locus_tag="PLH85_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB66073.1"
                     /translation="MDRTLLLHVALLLGLTALAVSAAPAAEPDGARQRALVHLVRQDC
                     GSCHGMRLSGGLGPDLRPARLAALPDESLVATILYGRPGTAMPGWQRFLSADEAGWIV
                     AQLKQGFPPLEP"
     gene            2809..3855
                     /locus_tag="PLH85_15765"
     CDS             2809..3855
                     /locus_tag="PLH85_15765"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNB66074.1"
                     /translation="MTQRIEWIDTAKGIGIYLVVTGHLLANNPPDSRLAEHLIWAFHM
                     PLFFFLSGLTHRPAAPGRVMEEISRTAKAIGMPYLFFSLLSIALWWTGTPADARGHGL
                     DDAFATLLSGVSGREKGLFYNIPLWFFPCLFSVRMLAVLAGAMLGENRTRLLVAACGL
                     AALAHLVLFPVLPRLPWNLDVAIVAGVFYAAGHRLAGTPALDSLLTHRFVAPVAGMSL
                     LLGGCLLNGRVDMNYRDFGQPLLFYVAAAGGIATTLWLSKRLEALRWLRTVGVASVVI
                     FPMHGLLQYFLPGNALATLTWYAFRATGSGVVGALLMGVLEVAMCLPFYGLLARHAPV
                     CIGRFPRRAARVAS"
     gene            complement(3925..4236)
                     /locus_tag="PLH85_15770"
     CDS             complement(3925..4236)
                     /locus_tag="PLH85_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB66075.1"
                     /translation="MKRSLFLTVLLATASTAAFADAPAAAQKAGCMACHATDKKVVGP
                     AFKDIAAKYKGQADAVAKLSDKVRKGGAGVWGPIPMPPNAADKINDADLKSTVEWILK
                     G"
     gene            complement(4307..6031)
                     /locus_tag="PLH85_15775"
     CDS             complement(4307..6031)
                     /locus_tag="PLH85_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNB66076.1"
                     /translation="MKQLWKTGAMALALLPLAVSHAFAEDAKTTTNAPEAKYQSAASP
                     LANVEMYQDINPKAPPMSKAEFDKARQIYFERCAGCHGVLRKGATGKPLTTDKTLAAG
                     TEYLKVFIKYGSPAGMPNWGTSGELTDDEVDLMARYVQQTPPQPPEFGLKDMKATWKV
                     IVPPEQRPKKKMNNYNIENIFSTTLRDTGEVALIDGDTKKIINIVKTGYAVHISRVSA
                     SGRYLFVIGRDAKINMIDLWMEKPDNVAEIRTGLEARSVETSKFKGYEDKLAVAGSYW
                     PPQYVIMKGDTLEPLKIMATRGMTVDDQEFHPEPRVASIVASHYKPEFVVNVKETGKT
                     LMVDYTDINAITTKEIPVAKFLHDGGLDSSKRYFMVAANQSNKIGAIDLKEGKLAGLI
                     EVGKIPHPGRGANFIHPKYGPVWSTGHLGDDSIALIGTDPVKHPQYAWKMVESIKGPG
                     GGALFIKSHPKSTHLYSDTPLNPDPKLSQSVVVFDTNNLAAGYKTLPIGEWAGLKDDG
                     AKRVVQPEFNKAGDEVWFSVWSAKNKESAIVVVDDKTLQLKTVIKDPRLITPTGHFNV
                     YNTQHDIY"
     gene            6186..7067
                     /gene="cobA"
                     /locus_tag="PLH85_15780"
     CDS             6186..7067
                     /gene="cobA"
                     /locus_tag="PLH85_15780"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNB66077.1"
                     /translation="MPPPVTAFALSDAGALRAFLDALVHRHAGGRHDAARPAAAGRPT
                     GRVALVGAGPGDPELLTLRAARLIAAADVLVVDHLVGDGVLALAHPGAERFYVGKEAG
                     HHTLPQEQINQLLVRLAREGRHVVRLKGGDPFIFGRGGEELEELLAAGIPFEVVPGIT
                     AACGIAAYAGIPLTHRDHAQSVVFATGHRRAGESSLDYVALSRPRQTAVIYMGVGMLL
                     SLCGALIAHGRDPATPAALIEKGTLPEQRVVTGTLATLPDLANLAGVRPPALLIVGEV
                     VSLAGKLAWTPSACSSV"
     gene            7139..7672
                     /locus_tag="PLH85_15785"
     CDS             7139..7672
                     /locus_tag="PLH85_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66078.1"
                     /translation="MSEDALPPLLLSLSGGDRRSIGRVADAVEAVLTDPALLATLFEG
                     MQRPDPVLRMRCADAAEKISARQPQWLLPFRDQLLDGLAADPQQEIRWHVAPMLLRLP
                     LSTAEADRVYAILVGYTADPSRIVRTEAMQALADLARRHPRFTDEVRRLLTALAASGT
                     PAMQARGRRLLARLNAA"
BASE COUNT         1130 a         2659 c         2619 g         1332 t
ORIGIN      
        1 tcggtggtgc cgagctgctc cgccaccgcc tggtagggcg cgtcacacag ggggaaaccc
       61 ccttgcagcg cgttgatcag ggcccggtcc agggcgtcga gggtcgccgc cgccttcatg
      121 aaacgcaggc cggctgcggt gtggcatccc ggtagcgggc gccgcactgc ttataacagt
      181 cggtggagaa cagcactgcg gacggatagc ccgcgacgcc ggcggcgtcc gcgacggtca
      241 tgcggcgcgc ttccacctcg gcgcgggaac gcccgtggag catacagaag agggagtagg
      301 gccactccgg cgcggttcgg cggcggtagc acaggctcac cccgtcctgg gcggccaggg
      361 cccggccggc agcgctcacg gcggcctccg gtacggccca cacgcacatg gcgttggcgt
      421 cccagcccag ctcgcgatgg cgcaccacca cgccgaagcg cctgagcgtg ccctcggcca
      481 gccacgccgc gagggtggcg aggacgtcct cctcgttcag accggccgcg tctgccaccg
      541 cccggtaggg ccgcggcacc cacggcagac cgtcctgcag ggcctcgacc aggcgccatt
      601 cggcggctga cagcgcacgc ttcatgccca ccggcgccgg cttgtggcga gtgcggcggc
      661 cgttggccag atcgaagccc agatcgatgt ggaactcctc caccagcggc agcgccacgg
      721 gcggacaacc ggtggcgctg ccgatggcgg ccagcaggcc gttcagggcc cgctcgtccg
      781 ccgccgccgc gacgaaccac aggttgaagc ggtgctcccg ccggtagttg tgattgacgc
      841 ccgcaaaggc gctgacgagg gccgccaccc gcggcagctc cgccggtggc actgccatcg
      901 ccgcgagggt gctggcaccc agacggttgg gcgcaaagac cggcccgacg cggctgatct
      961 tgcccgaatc gcgcaaccgg gcgaggcgct gcatcacctc gtcggcaccg cagcccaggt
     1021 gggcggcgag cgccgcccag ggctcggcac gcagcggaaa gccgcgctgg aagtcgttaa
     1081 gcaggtggaa gtcgcgggcg tctgcggtga gcatgattca gaatccgatg cgctggccgc
     1141 gggccgtgaa gaagatcccg gacggtgccg ccgcgggcag ctcggtgcgc gtcgcgaagg
     1201 tggcggtgtc caccacgctg acccggtcgc tgtcgcggct ggaaatccac acttcctcgc
     1261 cccgcggcgt gaattccagg tgcagcaccg ccttgccggg gctgaagctg tggatcacgc
     1321 ggcgctccac ggtgtcgatg acctgcaccc agtcgttgtc gggaaaggca aaattcaccc
     1381 agatctggcg accgtccgga cgcgccatga cgaacaccgg ctggcccttg acggggatgc
     1441 gcgcggtctc cgcccaggtg cgcgtgtcca ccgccagcac ctcgtgacgg cccaccgccg
     1501 gcagccacgc ctgccccgcc gccagggacc agccgcgcag gtggggcatc ttgaagacgg
     1561 gcagcttctc ctcgccgcgg ccatagccgg cgaggatcct ggtgaccccc ttttcgggct
     1621 gccacaggtc gaggagagag actccgtcct cgccgaagag cccggccagg aagtagcgtc
     1681 cgtccggggt caccagcccg tcatagggct gccggccggc cgggtagcgg gtggtcaccg
     1741 gatggcgcgg gtcgctgaaa tcgctcacgc ggatctcgtc gccgtcgaag agggcataca
     1801 cgaagcgccg ccccggtgca tccgccagcc ccaccacgcg ggagaacctg cccggcgcaa
     1861 cctcggcggg cacgtcggcg aggagttcca gggtgtccgc atcnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnaccacct gcacctggcc gctggcccgt tcgatgatca
     2041 cgcccagatc gccggtgccg cgcagttcat cgcagcgcga cgcgggcgcg gcgcaggccg
     2101 tcaggccaag ggccagcgca gcgacggaaa gccagtgaag ggtcttcatc tagggctcca
     2161 ggggcggaaa gccctgtttg agctgcgcca cgatccagcc tgcctcgtct gcgctgagga
     2221 agcgttgcca gcccggcatg gcggtgccgg gacggccgta caggatggtc gccaccaggg
     2281 actcgtcggg cagggcggcc agccgagccg gacgcagatc ggggccgagt ccgccggaca
     2341 ggcgcatgcc gtggcaggag ccgcagtcct ggcgtaccag atgtaccagg gcccgctggc
     2401 gtgcgccgtc cggttcggcg gccggcgcgg cactcaccgc cagggccgtc aggccaagca
     2461 gcagggccac gtggaggagc agggtacggt ccatggacag gctatccgca attcgatggg
     2521 cgtacggtac ggtcaaggcc cggtcgcctg ccttgacagg catcaagggc gggcgggcag
     2581 gcctgcgaag cctccggcat ggaccgatcc ttgacgatct cggcagtggc cgaaagcggc
     2641 ggggcgccac cccgaacccg cgggtgtccg gcgcgcgctc ggcccgcaac ccgcaggccg
     2701 acgccagcgt cccgagctga ggtagcatac gttccggccg gccttgtccc ggcgccctcc
     2761 gccgcccttt ccttcctgca gcacggcccc gcgccgaccg aacaccccat gacgcaacga
     2821 atcgagtgga tcgacaccgc caaagggatc ggcatctacc tggtggtcac cggccacctg
     2881 ctcgccaaca atccaccgga ttcgcgcctg gcagagcatc tgatctgggc tttccacatg
     2941 ccgctgttct tcttcctgtc cggcctcacg caccgtccgg cggcgccggg ccgggtgatg
     3001 gaggagatct cgcggacggc gaaagccatc gggatgccct acctgttctt cagcctgctg
     3061 tcgatcgccc tgtggtggac cggcacgccg gccgacgccc gcggacacgg actggacgac
     3121 gccttcgcga ccctgctctc tggcgtgtcg ggacgcgaaa aagggctctt ctacaacatt
     3181 cccctgtggt tctttccctg cctgttcagc gtccggatgc tggccgtgct ggcgggcgcc
     3241 atgctcggcg agaatcgcac gcgcctcctc gtggcggcct gcggcctcgc agcccttgcc
     3301 cacctggtcc tgttcccggt gctgccgcgc ctcccctgga acctggatgt ggccattgtc
     3361 gcgggcgtgt tctatgccgc aggccatcgg ctggccggaa cgccggcact ggacagcctg
     3421 ctgacccacc ggttcgtggc acccgtggcc ggcatgagcc tgctgctggg cggctgtctc
     3481 ctcaacgggc gggtggacat gaactaccgg gacttcggtc agcccctgct gttctacgtc
     3541 gcggccgccg gcgggatcgc caccaccctg tggctgtcaa agcgcctcga agcgctgcgc
     3601 tggctgcgga ccgtcggcgt cgcgtcggtg gtgatctttc caatgcacgg gctgctgcag
     3661 tatttcctgc ccggcaacgc cctggcgacc ctcacctggt atgccttccg ggcgactggc
     3721 tcgggcgtcg tgggggcgct gctcatgggt gtgctggaag tggcgatgtg cctgccgttc
     3781 tacggactgc tggcgcgcca tgcaccggtg tgcatcgggc gtttcccgcg ccgtgcggca
     3841 cgggtcgcgt cgtgaatcga ttggcccgga tgaaacggaa cggggcggcc agtgccgccc
     3901 cgcatccggc cgatcaggca cgcctcaacc cttcaggatc cactccacgg tggacttcag
     3961 gtcggcgtcg ttgatcttgt cggcagcgtt gggcggcatg gggatcggac cccacacacc
     4021 ggcgccgccc ttgcggacct tgtcgctcag cttggcgacg gcgtcggcct ggcccttgta
     4081 cttggcggca atatccttga acgcgggacc cacgaccttc ttgtcggtgg catgacaggc
     4141 catgcagcca gccttctggg cggcggcagg tgcatctgca aacgccgcag tgctggcggt
     4201 ggcaagcagg acggtcagaa acagcgaacg cttcatagtt tattctccca tctgacatgt
     4261 tggcaggacc gcggccggat cgaaccggcc gcggacggca cgacgatcag tagatgtcgt
     4321 gctgcgtgtt atacacgttg aagtgacccg tcggggtgat cagacgggga tccttgatga
     4381 cggtcttgag ctgcagggtc ttgtcgtcca ccaccacgat ggcggattcc ttgttcttgg
     4441 cgctccacac cgagaaccag acctcgtcgc ccgccttgtt gaactccggc tgcaccacgc
     4501 gcttggcgcc gtcatccttc agaccggccc attcgccgat gggcagggtc ttgtaaccgg
     4561 ccgccagatt gttggtgtcg aacacgacca ccgactggga cagcttggga tccgggttga
     4621 gcggcgtgtc ggagtacagg tgggtggact tcgggtggct cttgatgaac agcgcgccgc
     4681 cgccggggcc cttgatggac tcgaccatct tccaggcgta ctgcgggtgc ttcaccggat
     4741 cggtgccgat cagggcgatg gagtcgtcgc ccaggtggcc ggtggaccac acggggccgt
     4801 acttcggatg gatgaagttg gcgccgcggc ccgggtgcgg gatcttgccc acttcgatca
     4861 ggccggccag cttgccttcc ttcaggtcga tcgcgccaat cttgttggac tggttggcgg
     4921 cgaccatgaa gtagcgcttg gagctgtcga ggccgccgtc gtgcaggaac ttggcaaccg
     4981 ggatttcctt ggtggtgatg gcgttgatgt cggtgtagtc gaccatcagc gtcttgccgg
     5041 tctctttcac gttcaccacg aactcgggct tgtagtggga ggccacgatg gaggcgacgc
     5101 gcggctcggg gtggaactcc tggtcatcca cggtcatgcc gcgggtggcc atgatcttca
     5161 gcggctcgag ggtgtcgccc ttcatgatca cgtactgggg cggccagtag gagccggcga
     5221 cggccagctt gtcttcatag cccttgaact tggaggtttc caccgagcgg gcttcgagac
     5281 cggtacggat ctcggcgacg ttgtcgggtt tctccatcca caggtcgatc atgttgatct
     5341 tggcgtcgcg gccgatcacg aacaggtagc ggccggaggc ggagacgcgg gagatgtgca
     5401 ccgcgtagcc ggtcttgacg atgttgatga tcttcttggt gtcgccgtcg atgagtgcaa
     5461 cttcacccgt gtcgcgcagg gtggtcgaga agatgttctc gatgttgtag ttgttcatct
     5521 tcttcttcgg ccgctgttcc ggcggcacga tgaccttcca ggtcgccttc atgtccttca
     5581 ggccgaattc cggcggctgc ggcggggtct gctggacgta gcgggccatc aggtccactt
     5641 cgtcatcagt cagttcgccg gacgtgcccc agttgggcat gcccgccgga gagccgtact
     5701 tgatgaagac cttcagatat tcggtccctg cggcgagggt cttgtccgtc gtcagcggct
     5761 tgccggtggc gcccttgcgc agcacgccgt gacagccggc acagcgctcg aagtagatct
     5821 ggcgcgcctt gtcgaactcg gccttgctca tgggcggggc cttgggattg atatcctggt
     5881 acatctccac gttggcaagc ggcgaagcgg ccgactggta tttcgcctcc ggcgcgttgg
     5941 tcgtggtctt tgcgtcttcg gcaaaagcat gcgacacggc cagcggcagc agggccaggg
     6001 ccatggcgcc ggtcttccat agttgtttca tcttgaatct cccttggttg atacccgatg
     6061 agcggccaat tccgcttggc gcgaaggttg cgctttcggg tgattcccct tccttgacag
     6121 gagtcaagtt cgccagcggg acccggctga tagattgcgt ccatcctgtt tcggggagtc
     6181 ccgtcatgcc gccgcccgtc accgcctttg ccctgtccga cgctggcgcg ctgcgcgcct
     6241 tcctcgatgc gctggtgcat cgtcacgcag gcggacgcca cgatgccgcc cgccccgctg
     6301 ccgccggacg ccccaccggg cgagttgcac tggtgggtgc cgggccgggc gatcctgagc
     6361 tcctcaccct gcgcgctgcg cgcctgattg ccgcggccga cgtgctggtg gtggatcacc
     6421 tggtcggtga cggcgtgctg gcgctggccc atcccggcgc cgaacgcttc tacgtgggca
     6481 aggaggccgg tcaccacacc ctgcctcagg aacagatcaa ccagctgctg gtccgcctgg
     6541 cccgcgaagg ccgccacgtg gtccgcctga aaggcggcga ccccttcatc ttcggacgcg
     6601 gcggcgaaga gctggaagaa ctcctcgccg cggggattcc cttcgaggtg gtgcccggca
     6661 tcaccgccgc ctgtggcatc gctgcctacg cagggattcc cctcacccac cgggatcacg
     6721 cccagtcggt ggtgttcgcc accggccatc gccgcgccgg cgaatcctcc ctcgactatg
     6781 ttgccctgtc gcgcccccgt cagaccgcgg tgatctacat gggtgtgggc atgctcctgt
     6841 ccctgtgcgg tgcgctgatc gcccacggcc gcgatcccgc cacgccggcg gcgctgatcg
     6901 aaaagggcac cctgccggaa cagcgcgtcg tcaccggcac cctcgccacc ttgcccgatc
     6961 tggccaatct ggccggcgtg cgcccgccgg cgctgctgat cgtcggtgaa gtcgtgtcac
     7021 tggccggcaa gctggcgtgg accccctccg cctgcagcag cgtctgaacc cgtcaccggc
     7081 atggggccgg aaaatgggct aggctggtgc gcttcggtcc tccccgtttc atccgcccat
     7141 gtccgaagac gccctcccgc cgctcctcct cagcctctca ggtggcgacc ggcgttccat
     7201 cggacgcgtg gcggacgcag tcgaggccgt cctgaccgac ccggccctgt tggcgaccct
     7261 cttcgagggc atgcagcgac ctgatccggt gctgcgcatg cgttgcgccg acgcggctga
     7321 aaagatcagc gcccgccaac cccagtggct gctgcccttc cgggaccagc tcctggacgg
     7381 gctggctgcc gatccccagc aggagatccg ctggcacgtg gcgccgatgc tcttgcgctt
     7441 gcccttgagc accgccgaag ccgaccgggt atacgcgatt ttggtgggct acaccgcgga
     7501 tcccagccgc atcgtgcgca ccgaggcgat gcaggcgctg gccgatctgg cccgccgcca
     7561 cccgcgcttc actgacgagg tgcgccgcct gctgacggcg ctcgccgcaa gcggtacccc
     7621 ggcgatgcag gcccgcggcc gtcggctgct cgcgcgcctg aacgccgcct gaggcccctc
     7681 cgggcgcttt ctcccgtcat cgccggcgca gcggattcca cgccatccgg cctgcttgat
     7741 acgagtcaag gacgtcgccc ccctcggccg cggaacatcg cggccaagct gcgccgtgca
     7801 gcgaagccat ccagggaggt tccatgagcg ggacattcac
//
LOCUS       DAOQMM010000135         7831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_4794_length_7831_cov_47.452803, whole genome shotgun sequence.
ACCESSION   DAOQMM010000135 DAOQMM010000000
VERSION     DAOQMM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_7831_cov_47.452803"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLH85_15790"
     CDS             complement(<1..259)
                     /locus_tag="PLH85_15790"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584312.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNB66079.1"
                     /translation="MDSDSRKQRGAAKTARIPIKIVPAEKLRKPEWIRIKLGAGAEIE
                     RFNEIKDTLREHKLHTVCEEASCPNIHECFGKGTATFMIMGD"
     gene            complement(279..890)
                     /gene="lipB"
                     /locus_tag="PLH85_15795"
     CDS             complement(279..890)
                     /gene="lipB"
                     /locus_tag="PLH85_15795"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNB66080.1"
                     /translation="MIVRHLGLVPYEPTWRAMQDFTATRDAATPDELWLLQHPPVYTL
                     GQAGKPEHLIRPTEIPLVKIDRGGQITYHGPGQVVGYLLLDLHRRHLKVREMVHQLEQ
                     ALIDCIADYGLDARRKDGAPGVYIDGAKIAALGLRVRNGCSYHGLSLNVDMDLTPFTW
                     INPCGYSGLQTIQLKDFGVTEAPDTVAERLLGHLQRHFPDPAA"
     gene            complement(887..1156)
                     /locus_tag="PLH85_15800"
     CDS             complement(887..1156)
                     /locus_tag="PLH85_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HNB66081.1"
                     /translation="MSEERQTLLEFPCAFPIKIMGARVDAFAQTVLDVVLRHDPGFDA
                     AAMEMRPSSKGTYLAITCTVTAISQTQLDNLYRELSSHPMVKVVL"
     gene            complement(1300..2424)
                     /locus_tag="PLH85_15805"
     CDS             complement(1300..2424)
                     /locus_tag="PLH85_15805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNB66082.1"
                     /translation="MLRRLFLIVLLCPVLAFAQLAPPAVNARAWLLLDVGSGQVLTAE
                     KPDERIEPASLTKLMTAYLTFSAVKAKTLSLDQTVPVSEKAWKISGSKMFIEPLKPVT
                     VRELIHGMIVQSGNDACVALAEAIAGSEENFVQLMNKEAARLGLKNTHFENSTGLPHP
                     AHLASVRDLAALASAIIRDFPEFYPIYSTKEYTYNKITQPNRNRLLFLDPTVDGVKTG
                     HTESAGYCLIASAKRNERRLISVVVGTTSDSVRAAESQKLLNWGYLAYDAVKVYSAKQ
                     PVQEFRLWKGAANSIKVGFLKDLVLSLPKGDADKLKMTVVSQEPLIAPVTQGQPLATL
                     QLTLDGKPVADYPLVALDDVPQAGWFGRVWDAIRLWFKSL"
     gene            2534..2851
                     /locus_tag="PLH85_15810"
     CDS             2534..2851
                     /locus_tag="PLH85_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66083.1"
                     /translation="MHTFRRLLSLLLILLTFTWAGAVASAEPPMADADGPVAPLLAHG
                     DAAAGGADADGDGLADVLEMPALPALLPIVGIAPRPLWVAVHPCLSPSLPLAPPAPPP
                     RLV"
     gene            2930..4174
                     /locus_tag="PLH85_15815"
     CDS             2930..4174
                     /locus_tag="PLH85_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB66084.1"
                     /translation="MLRILAVAALAALPVLSAAADGPAFTLSALREAARAGHPVLAAA
                     RAQVEGGRAQVDSAAAYPNPELEVLSGRTRARLPGVNAGTVVSVAVTQRVDVPWQRDA
                     RIDVAGHGLTALEAGRRGVENELLARLDQRFYELLRREAEVRAAREDRQLTEQIRSRV
                     AVRVETGEAPRYELFKADTELLNARKTEDSASLRVEQARAALRALVGAALPARFSVIG
                     SLGDRAAVATLDALRDAVMSGNPDLARLRAEVARAARQLDLERLRRQPELAVKVADER
                     DSELRDTRVGLVVSVPLWDRRRGPVAEAAARLEAARSELSAQELAVQQALEGAWRQYE
                     ITLTQVIALENGILREAEAALKVAEAAYRFGERGILDYLDAQRVFRIARNELIAARYD
                     LQLAVIEIERLRAAQPWSGATP"
     gene            4171..5385
                     /locus_tag="PLH85_15820"
     CDS             4171..5385
                     /locus_tag="PLH85_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB66085.1"
                     /translation="MNLPSSFKTATVPAVLLGLFLTACQDARPPAAPAAAARPSPSSA
                     LGEVPGSDTDAPPADPLVVAVPPRFGVALQVLPVASAALSDTLRVAGKVDFDERRVSR
                     IGATVTGRVVEIQAHLGQTVHAGDTLAIINSTELGQAQIVYLKARAQADLQSRSVERA
                     RQLFAADVIGRAELQRRESELAIATAEQRGAEDQLQVLGMSAGAIRRLGAHGAINSVT
                     PVVSATAGTVVERKVTQGQVVQPSDSLFMVADLSQVWVTAEVPEQQAALVKSGQSVEI
                     EVPALGARLTGKLIYVADTVNPDTRTVTVRSAVDNRARQLKPAMLATMLIQAAPAERL
                     VVPAQAVVRDANTDNVFVEIAPQRFRLTPVRLGPEADGRRAVLEGLKAEQRILVAGAF
                     HLNNERKRKELE"
     gene            5382..>7831
                     /locus_tag="PLH85_15825"
     CDS             5382..>7831
                     /locus_tag="PLH85_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB66086.1"
                     /translation="MIESLVRTALQQRLVVVVVAAILLVFGLNAARKLSVDAFPDVTN
                     VQVQIATEAKGRSPEEVERFVTVPLEIAMTGLPGLEEMRSLNKPGLSLITLVFTDATD
                     VYFARQLVMERLLEVGARMPAGVVPVLGPVSTGLGEVYQYTLDHPEDGDRTLTEKELT
                     ERRIVQDWVVRPLLRSIPGVAEINSQGGYARQYQVLVNPDRLRHYQLTVRDVYEAVAR
                     NNANSGGGVLPQYAEQYLIRGVGLVKSLSDIGAIVLKEIGGTPVFVRDVAEVSFGHEV
                     RQGAVVKNGSTEAVGGVVMMLRGGNAKEVVSRIKARVAEINAKGMLPDGLRIVPYYDR
                     SDLVDAALWTVSKVLIEGIVLVVIVLFLFLGDVRSSLIVVATLVLTPLVTFMVMNRYH
                     LSANLMSLGGLAIAIGLMVDGSVVVVENAFARLGHARSGESRARIIFDAVKEVATPVI
                     FGVGIIILVFLPLMTLQGMEGKMFAPLAYTIAIALAVSLVLSLTLTPVLSSYLLKGGA
                     EHDTWVIARLKAPYLKMLHWAVAHSRATALGGVVLLVATLAMFPLLGTAFIPEMKEGS
                     LVPGINRVPNISLDESVKMEMQAMRRVMEVPGVRSAISGVGRGESPADPQGQNESTPI
                     VSLKPRDEWPAGWTQDDIADAIRDALQELPGVQVVMAQPISDRVDEMVTGVRSDVAIK
                     VFGDDLESLRLKAEEIARVAGGITGATDIRVERVSGQQYLSIEIDRAAIARHGLNVSD
                     VNDVIEASIGGRQATEIYEGERRFAAVVRLPEAFRDNAEKIGKILVSAPGGVQVALEN
                     LATIRIADGPAQISRELA"
BASE COUNT         1119 a         2558 c         2923 g         1231 t
ORIGIN      
        1 tgtcgcccat gatcatgaag gtggccgtgc ccttgccgaa gcattcgtgg atgttggggc
       61 aggaggcttc ctcgcagacg gtgtgcagct tgtgctcgcg cagggtgtcc ttgatctcgt
      121 tgaagcgctc gatctcggcg ccggcgccga gcttgatgcg gatccactcg ggcttgcgca
      181 gcttttcggc gggcacgatc ttgatgggaa tgcgggcggt cttggccgca ccgcgctgct
      241 tgcggctgtc ggaatccatg gggctgcgtc ctctggtgtc aggcagcggg atcgggaaaa
      301 tgccgctgca ggtggccgag caggcgctcg gcgacggtgt cgggggcttc ggtgacgccg
      361 aagtctttga gctggatggt ctggagccca ctatagccgc agggattgat ccaggtaaat
      421 ggtgtcaaat ccatatcgac attgagggac aaaccgtggt aggagcagcc gttcctgacc
      481 cgcaggccga gggcggcgat cttggcgccg tcgatgtaca cgcccggcgc gccgtccttg
      541 cggcgggcgt cgaggccgta gtcggcgatg cagtcgatga gggcctgttc gagctggtgc
      601 accatctcgc gcaccttgag gtggcggcgg tgcaggtcga ggagcaggta gcccaccacc
      661 tggccggggc cgtggtaggt gatctggccg ccccggtcga tcttgaccag ggggatctcc
      721 gtggggcgga tcaggtgttc gggcttgccg gcctggccga gggtgtagac cggcgggtgc
      781 tggagcagcc acagctcgtc cggcgtggcg gcgtcgcggg tggcggtgaa gtcctgcatg
      841 gcccgccagg tgggctcgta ggggaccagg ccgaggtgcc ggacgatcac aggacgacct
      901 tcaccatggg atgggacgac agctcgcggt agaggttgtc gagctgggtc tgggagatgg
      961 cggtcaccgt gcaggtgatg gccaggtagg tgcccttgga ggacgggcgc atctccatgg
     1021 cggcggcgtc gaagccgggg tcgtggcgca ggacgacgtc cagcaccgtc tgggcgaagg
     1081 catccacccg cgcgcccatg atcttgatgg ggaaggcgca ggggaattcg agcagggtct
     1141 gacgttcttc ggacatgggc gcgggccggg tgcggggtcg ggatctttcc aagatgggac
     1201 cgccgcggcg aaagttcaag ggcgggctgc gtgcaccgcg tcaggcggcc cgcggtccgc
     1261 cccgggccga ccgggcgcgg acccggccgg cggggggatt cacaggctct tgaaccacag
     1321 gcggatggcg tcccagacgc ggccgaacca gccggcctgg ggcacgtcgt cgagggccac
     1381 cagcgggtag tcggcgacgg gcttgccgtc cagggtgagc tgcagggtgg ccaggggctg
     1441 gccctgggtg accggcgcga tcaggggttc ctggctgacc acggtcatct tcagcttgtc
     1501 ggcgtcgccc ttgggcaggg acagcaccag gtccttgagg aagccgacct tgatgctgtt
     1561 ggcggcgccc ttccacaggc ggaattcctg caccggctgc ttggcggaat agaccttgac
     1621 cgcgtcgtag gccaggtagc cccagttgag gagcttctgg gattcggcgg cgcgcacgct
     1681 gtcgctggtg gtgcccacca ccacggagat gaggcggcgc tcgttgcgct tggcggaggc
     1741 gatcaggcag tagccggcgc tctcggtgtg gccggtcttg acgccgtcca cggtggggtc
     1801 gaggaagagc aggcggttgc ggttgggctg ggtgatcttg ttgtaggtgt attccttggt
     1861 ggaatagatc gggtagaact cggggaagtc gcggatgatg gcgctggcca gggccgccag
     1921 atcgcgcacc gaagccaggt gggccgggtg gggcagaccg gtggagttct cgaagtgggt
     1981 gttcttgaga cccaggcgcg cggcctcctt gttcatcagc tgcacgaagt tctcttcgga
     2041 accggcgatg gcctcggcca gggcgacgca ggcgtcgttg ccggactgga cgatcatgcc
     2101 gtggatcagc tcgcgcacgg tgaccggctt gaggggctcg atgaacatct tggagccgct
     2161 gatcttccag gccttctcgg agaccggcac ggtctggtcg agggacaggg tcttggcctt
     2221 gaccgccgag aaggtgaggt aggcggtcat cagcttggtg agggacgccg gctcgatgcg
     2281 ctcatcgggc ttttcggcgg tgaggacctg gccggagccc acgtcgagga gcagccaggc
     2341 gcgggcattg acggccggcg gggcgagctg ggcgaaggcg aggacggggc acagcaggac
     2401 gatcaggaag aggcggcgga gcatgggaag ggggggtgaa gacgcggaag ggcgcattat
     2461 agtcaccgtg accggccggc tcccagcacc ccgtgcggaa cttgttaccg tatgttcggt
     2521 ctggattgtt gcaatgcaca cattccgtcg cctgctctcc ctgctgctga tcctgctcac
     2581 cttcacgtgg gcgggtgcgg tggcgtccgc cgagccgccc atggccgacg cggacggccc
     2641 ggtggcgccc ttgctcgccc atggcgacgc ggcggccggc ggggccgatg cggacggcga
     2701 cggtttggcc gacgtcctcg agatgccggc cctgccggcg ctgctgccga tcgtgggcat
     2761 cgcgccgcgg cccctgtggg tcgccgtgca tccctgcctg tccccgagtc tccccttggc
     2821 gccgccggcg ccccctcccc gcctcgtctg atccggcgcc cccgtggcgc cgaccgcagg
     2881 tgcgcacgcc gggagggcgt gcgcggttcg agatccgacg aggacatcca tgctacgaat
     2941 ccttgcagtg gccgccctgg cggccctgcc ggtcctgtct gcggcggcag acggaccggc
     3001 cttcaccctg tccgccctgc gcgaggcggc gcgggccgga caccccgtcc tggccgccgc
     3061 ccgcgcccag gtggagggcg gtcgcgccca ggtggacagc gccgccgcct accccaaccc
     3121 ggagctggag gtgctctccg gccgtacccg cgcccgtctg cccggggtga acgccggcac
     3181 cgtggtgagc gtggcggtga cccagcgggt ggacgtcccg tggcagcggg acgcccgcat
     3241 cgacgtggcc ggacacggcc tgacggccct ggaggccggc cgccgcggtg tcgagaacga
     3301 gctgctggcc cgcctggacc agcgcttcta tgaactcctg cggcgcgagg cggaggtccg
     3361 cgccgcccgc gaggaccggc agctcaccga acagatccgc agccgcgtgg ccgtgcgcgt
     3421 ggagaccggc gaggcgcccc gctacgagct cttcaaggcc gacacggagc tcctcaatgc
     3481 gcgcaagacc gaggacagcg ccagcctgcg ggtggagcag gcccgcgcgg ccttgcgcgc
     3541 cctggtcggt gcggcgctgc cggcccgctt ctcggtgatc ggcagcctgg gggaccgggc
     3601 ggcggtggcc acgctggacg ccttgcggga cgcggtgatg agcggcaacc cggacctcgc
     3661 ccgtctgcgc gccgaggtgg cccgtgcagc ccgccagctg gatctggagc gcctgcgccg
     3721 ccaacccgag ctggccgtga aggtggcgga cgaacgtgac agcgagctgc gcgacacccg
     3781 cgtgggcctg gtggtgagcg tgcccctgtg ggaccgccgc cggggcccgg tggcggaggc
     3841 ggcggcccgc ctggaggccg cccgcagcga gctgtctgcc caggagctgg ccgtgcagca
     3901 ggccctggaa ggcgcctggc gccagtacga gatcaccctc acccaggtca tcgccctgga
     3961 gaacggcatc ctgcgggagg ccgaagccgc cctgaaggtg gccgaggccg cctaccgctt
     4021 cggcgagcgc ggcatcctcg attacctgga cgcccagcgc gtgttccgca tcgcccgcaa
     4081 cgaactcatc gccgcccgtt acgacctgca gctcgccgtc atcgagatcg agcgcctgcg
     4141 ggcggcccag ccctggtccg gagcgacacc atgaacctgc cctcttcctt caagaccgcg
     4201 accgtgccgg ctgtgctgct cggtctgttc ctgaccgcct gtcaggacgc caggccgcct
     4261 gccgcgccgg ccgccgcggc ccgtccctcg cccagctcgg ccctgggcga ggttccgggc
     4321 agcgacaccg acgcgccgcc ggccgacccc ctggtggtgg cggtgccgcc ccgattcggt
     4381 gtggcgcttc aggtgctgcc ggtggcgtcc gcggccctct ccgacaccct gcgggtggcc
     4441 ggcaaggtgg actttgacga acgccgggtg agccgcatcg gcgcgacggt gaccggccgc
     4501 gtggtggaga tccaggcgca tctgggccag accgtgcatg ccggcgatac cctggcgatc
     4561 atcaacagca cggagctggg gcaggcccag atcgtctatc tgaaagcccg cgcccaggcc
     4621 gacctgcagt cacgcagcgt ggagcgggcg cgacagctgt tcgctgccga cgtgatcggt
     4681 cgggccgagc tgcagcggcg cgagagcgag ctggccattg ccaccgcgga gcagcgcggg
     4741 gcggaggacc agctgcaggt gctgggcatg tcggcgggcg cgatccgccg cctgggcgcc
     4801 cacggggcca tcaattcggt gaccccggtg gtgtccgcca ccgcgggcac cgtggtggag
     4861 cgcaaggtga cccagggcca ggtggtgcag ccttcggaca gcctgttcat ggtggccgac
     4921 ctgtcccagg tgtgggtcac cgccgaggtg ccggagcagc aggcggccct ggtgaagagc
     4981 ggccagagcg tggagatcga ggtgcccgcc ctgggcgccc gcctcaccgg caagctgatc
     5041 tacgtggccg acacggtcaa tccggacacc cgcacggtca ccgtgcgcag cgcggtggac
     5101 aaccgcgcgc gccagttgaa gccggccatg ctggcgacca tgctgatcca ggccgcgccg
     5161 gccgagcgtc tggtggtgcc ggcccaggcc gtggtgcgcg atgcgaacac cgataacgtc
     5221 ttcgtggaga tcgcgcccca gcgcttccgc ctcacgccgg tgcgcctggg gcccgaggca
     5281 gacggccgcc gcgcggtgct ggagggcctc aaggcggagc agcgcatcct cgtggctggc
     5341 gctttccacc tcaacaatga gcgcaagcgg aaggagctgg aatgatcgaa tccctcgtcc
     5401 gcacggcgct gcagcagcgc ctggtggtgg tcgtggtggc ggcgatcctg ctggtcttcg
     5461 gcctcaacgc ggcgcgcaag ctgtcggtgg atgcctttcc cgacgtgacc aacgtgcagg
     5521 tgcagatcgc caccgaagcc aaggggcgtt cgcccgagga ggtggagcgc ttcgtgaccg
     5581 tgccgctgga gatcgccatg accggcctgc ccggcctgga ggagatgcgc tccctcaaca
     5641 agcccggcct gtccctcatc accctggtgt tcaccgacgc caccgacgtg tatttcgccc
     5701 gccagctggt gatggagcgc ctgctcgagg tgggcgcgcg gatgcccgcg ggcgtggtgc
     5761 cggtgctggg cccggtgtcc accggtctgg gcgaggtgta tcagtacacc ctggatcatc
     5821 cggaggacgg cgaccggacc ctcaccgaga aggaactgac ggaacgccgc atcgtccagg
     5881 actgggtggt gcgcccgctg ctgcgctcca ttcccggcgt ggcggagatc aactcccagg
     5941 ggggctatgc gcgccagtat caggtgctgg tgaacccgga ccgcctgcgt cactaccagc
     6001 tgacggtgcg cgacgtgtac gaggcggtgg cgcgcaacaa cgccaacagc ggcggcggcg
     6061 tgctgcccca gtacgccgag cagtacctga tccgcggcgt cggcctggtg aagagcctgt
     6121 cggacatcgg cgccatcgtg ctcaaggaga tcggcgggac gccggtgttc gtgcgcgacg
     6181 tggcggaggt gagcttcggc cacgaggtgc gccagggcgc cgtggtgaag aacggcagca
     6241 ccgaggccgt gggcggcgtg gtgatgatgc tgcgcggcgg caacgccaag gaggtggtga
     6301 gccgcatcaa ggcgcgggtg gccgagatca acgccaaggg catgctgccg gacgggctcc
     6361 gcatcgtgcc ctactacgac cgctccgatc tggtggacgc ggccctgtgg accgtgagca
     6421 aggtgctcat cgaaggcatc gtgctggtgg tgatcgtgct gttcctgttc ctcggcgatg
     6481 tgcgctccag cctcatcgtg gtggccacgc tggtgctcac gcccctcgtc accttcatgg
     6541 tgatgaaccg ctaccacctg tcggccaatc tcatgtccct gggcgggctg gccatcgcca
     6601 tcggcctgat ggtggatggc tcggtggtgg tggtggagaa cgccttcgcg cgcctgggcc
     6661 atgcccgcag tggcgagagc cgggcgcgga tcatcttcga cgcggtcaag gaggtggcca
     6721 cgccggtgat cttcggtgtg ggcatcatca tcctggtctt cctgcccctc atgaccctgc
     6781 agggcatgga gggcaagatg ttcgcgcccc tcgcctacac catcgccatt gccctggcgg
     6841 tgtcgctggt gctgtccctg acgctcacgc cggtgctgtc gtcctacctc ctcaagggcg
     6901 gtgccgagca cgatacctgg gtcatcgccc ggctcaaggc gccctacctg aagatgctcc
     6961 actgggcggt ggcccacagc cgcgcgacgg cgctgggggg cgtggtgctg ctggtcgcca
     7021 cgctggccat gttccccctg ctgggcaccg ccttcatccc ggagatgaag gagggctcgc
     7081 tggtgccggg catcaaccgg gtgcccaaca tctccctgga tgagtcggtc aagatggaga
     7141 tgcaggccat gcgccgggtc atggaagtgc ccggcgtgcg ctcggcgatt tccggcgtgg
     7201 gccgtggcga gtcgccggcg gatccccagg ggcagaacga gtcgaccccc atcgtgagcc
     7261 tcaagccccg cgacgagtgg cccgccggat ggacccagga cgacatcgcc gacgccatcc
     7321 gcgatgccct ccaggaactg cccggcgtcc aggtggtgat ggcccagccc atctccgacc
     7381 gggtggacga gatggtgacc ggggtgcgct ccgatgtggc catcaaggtc tttggcgacg
     7441 acctggagtc cctgcgcctc aaggccgagg agatcgcccg ggtggcgggt ggcatcaccg
     7501 gcgccacgga catccgggtg gagcgggtga gcgggcagca gtacctgtcc atcgagatcg
     7561 accgggccgc catcgcgcgc cacggcctca acgtgtccga cgtaaacgac gtgatcgagg
     7621 cgtccatcgg cgggcgtcag gccaccgaga tctacgaggg cgagcggcgc tttgcggccg
     7681 tggtgcgcct gcccgaggcc ttccgcgaca acgccgagaa gatcggcaag atcctcgtca
     7741 gcgcgccggg gggcgtgcag gtggccctcg agaatctggc cacgatccgc atcgccgacg
     7801 gtcccgccca gatctcccgg gagctggcca a
//
LOCUS       DAOQMM010000136         6981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_5846_length_6981_cov_49.077101, whole genome shotgun sequence.
ACCESSION   DAOQMM010000136 DAOQMM010000000
VERSION     DAOQMM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5846_length_6981_cov_49.077101"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLH85_15830"
     CDS             complement(<1..374)
                     /locus_tag="PLH85_15830"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNB66087.1"
                     /translation="MTRLALIAAVARNGVIGVANRLPWHLPEDMKFFRETTRGQPVIM
                     GRKTWESLPDTFRPLPGRHNIVVSRNPDFRAAGATVVTSLPDALTAAGDADTAYLIGG
                     AELYRQALPLADRLVLTEIDRD"
     gene            complement(371..1165)
                     /locus_tag="PLH85_15835"
     CDS             complement(371..1165)
                     /locus_tag="PLH85_15835"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNB66088.1"
                     /translation="MRQYLDLMRHVLEHGHRKDDRTGTGTLSVFGWQMRFNLADGFPL
                     LTTKKLHTRSIIHELLWFLQGDTNIRYLKDHGVSIWDEWADDQGDLGPVYGKQWRRWE
                     TADGRSIDQIAQLIAGLKGNPDSRRHIVSAWNPGDVERMALPPCHALFQFYVAGGRIS
                     CQLYQRSADIFLGVPFNIASYALFTMMVAQVCGYAPGDFVWTGGDCHLYANHLDQAHL
                     QLSREPRPLPTLRINPDVKDLFAFRFEDFSLEGYDPHPHISAPVAV"
     gene            1335..2795
                     /locus_tag="PLH85_15840"
     CDS             1335..2795
                     /locus_tag="PLH85_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66089.1"
                     /translation="MVLGEKDREPHPLLDEQVLRRVLTDISTSAAPAALGELIRLLAS
                     MPDVRDMDVRNTARQVFALDEAAQAQVRQAAGLYLSNAYSARSVRYKALGQDFHAFLG
                     QVYDLLMSGLIGDFTIPRDDPLRAEILLRTVRTAAGEFKWAAFDYQSLSPEAWRRAMS
                     AYRLALSAGVADLPVRLREGRESRTTVSREFARLVAMQCAGFEQLSPERIEAADKLMR
                     FVQHALRLTGEPLEGSQFSVDLDGFERPRRLLNLPEAAGTLRFFRPGDAVPVLGDLAG
                     SVLLPGGHAAFAGTNPVEIGAAIAHLNRQWGARAPMRQYRRHPMRGEVSLSTGLGLIR
                     SLISGEAQFRPAPAWHLVDASRDGFCLASRLFDGEVCRVGTLVGAYLGEWGTWTLAVV
                     RRVQHSERQGTSIGLQILTSGPRPAALDDGSRSWLGVLCDPVVRGRALRIVCEPGFTG
                     LGGRLFVKQGPRTVKADVEHLVARGPGFEVWGCKVR"
     gene            3142..4848
                     /gene="leuA"
                     /locus_tag="PLH85_15845"
     CDS             3142..4848
                     /gene="leuA"
                     /locus_tag="PLH85_15845"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNB66090.1"
                     /translation="MLKNPNTKYSPFPPVALADRQWPSRTITHPPVWMSTDLRDGNQA
                     LFEPMNAARKLQMFRQLVAIGFKEIEVAFPSASQTDFDFVRQLIEGGEIPDDVTIEVL
                     SQAREHLIRRTMESLRGARRAIVHIYNATSRNFRETVFGMSRDEVKAMAVDSVRLVKT
                     LAAEQPTTEWVLEYSPETFTATELDFAREVCDAVVAAWDPRPGEKVILNLPSTVEMAT
                     PNVYADQIEWMHRHLARREQIILSVHPHNDRGTAVAAAELAVLAGAERVEGCLFGNGE
                     RTGNVDLVTLALNLYTHGIDPGLDFSDINTVVRVAEACTQLPVHPRHPYAGDLVFTAF
                     SGSHQDAIKKGLQIQQPDTLWQVPYLPVDPADLGRTYDSVIRVNSQSGKGGVAHLLES
                     AYGLVMPRRMQVEFSGAVQRLTDETGQEVDAHQLWQLFCREYLDADAPVRYGDHHLYE
                     ADGRQGIRIEAHIDDQAQVLKGEGNGPVEAAVAALRVPVRVQSFEERSMGEGADAVAM
                     AIVEVAMPGVAGTCFGAAMHRNTVTASVRAVVAAVNRILARQGGGANPGAVEAVQAGC
                     AA"
     gene            complement(4913..5476)
                     /locus_tag="PLH85_15850"
     CDS             complement(4913..5476)
                     /locus_tag="PLH85_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNB66091.1"
                     /translation="MNAHLLTHSRRVALRGLAAGVGLALMLTPPAQADDLQLLIKHGT
                     VGGRFERNGIGLRFGTLWTSMWGPWTAALKPELEFSRLRYSGTADAPHSLDEAGILGV
                     LRVTHPGAGLQAYVEAGLGGALFSHDRLGDKTFSTHFQFSEHVGAGVTLGGRASLGWQ
                     FSHYSNADIRLPNDGLDVHQIVLAIRF"
     gene            complement(5640..6923)
                     /locus_tag="PLH85_15855"
     CDS             complement(5640..6923)
                     /locus_tag="PLH85_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB66092.1"
                     /translation="MKIETLAVHAGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVAGNIYTRIMNPTQAVLEARVAAMEGGIAGLALASGMAAITYAIQTIAEAGDNIVS
                     ASTLYGGTYNLFAHTLPQIGIQVRFADYRDPDSFAPLIDERTKAIYCESVGNPLGNIT
                     DFEALAAIAHAHGVPLIVDNTVPSPYLCRPIEHGADIVVHSLTKYLGGHGNSIGGLIV
                     DSGKFPWAEHKARFRRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNMGAAISPFN
                     VFLILQGIETLTLRMDRICKNTRKVAEFLKGHAKVAWVNYAGLPDHPDHALVQKYMGG
                     RASGILNFGVKSADGDGKAAGARFQDALQLVTRLVNIGDSKSLACHPASTTHRQLSPD
                     EMARAGVSEDMVRLSIGIEHIDDILADLDQALAAV"
BASE COUNT         1067 a         2187 c         2506 g         1221 t
ORIGIN      
        1 aaatcccggt cgatttctgt gagcaccagc cggtcggcca ggggcagggc ctggcggtag
       61 agctcggcgc cgccgatcag gtaggcggtg tccgcgtcgc ccgcggcggt cagcgcatcg
      121 ggcaggctgg tcaccacggt ggcgccggcc gcacggaagt ccgggttgcg gctcaccacg
      181 atgttgtgtc ggccgggcag ggggcggaag gtgtcgggca gggactccca agtcttgcgg
      241 cccatgatca ccggctggcc gcgggtggtc tcgcggaaga acttcatgtc ctccggcagg
      301 tgccagggca ggcggttggc gacgccgatg acgccgttgc gcgccaccgc ggcgatcagg
      361 gcgaggcggg tcataccgcc accggcgcgg agatgtgggg gtgcgggtcg tagccttcga
      421 gggagaagtc ctcgaagcgg aaggcgaaca gatccttcac gtccgggttg atgcgcaggg
      481 tcggcagcgg ccgcggctcg cgggacagct gcaggtgggc ctggtccagg tgattggcgt
      541 acaggtggca gtcgccgccg gtccatacga aatcgcccgg cgcgtagccg cacacctggg
      601 ccaccatcat cgtgaacagg gcgtagctgg cgatgttgaa gggcacgccg aggaagatgt
      661 cggcgctgcg ctgatacaac tggcaggaga tgcgtccgcc ggccacgtag aactggaaca
      721 acgcgtggca gggtggcagg gccatgcgct ccacgtcacc ggggttccat gcggagacga
      781 tgtggcggcg ggagtccggg ttgcccttga ggccggcgat gagctgggcg atctggtcga
      841 tgctgcgccc gtccgcggtt tcccagcgcc gccactgctt gccgtagacc gggcccaggt
      901 cgccctgatc gtccgcccat tcgtcccaga tcgacacgcc gtggtctttg aggtaacgga
      961 tgttggtgtc gccctgcagg aaccacagca gctcgtggat gatggagcgg gtgtggagct
     1021 tcttggtggt gagcagcggg aagccgtcgg ccagattgaa gcgcatctgc cagccgaaga
     1081 cggacaaagt gccggtgccg gtccggtcat ccttgcggtg gccgtgctcc agcacgtggc
     1141 gcatgaggtc aagatactgt cgcatggtga gggcaggcaa agccgccagt gtacctgatg
     1201 ccacggcgtt cgcaccgccg ccaggcggcc cgcagcgcga acatgtcggc acccggtacg
     1261 tgctatccgt gtgctaacat cttctgaaat tgacaaaact ttacgtgggc ggcggcggcc
     1321 cgcgaggtga gaaaatggtg cttggggaga aagaccggga accgcatccc ctgctcgacg
     1381 agcaggtctt gcgccgcgtg ctcaccgaca tctccacgag tgctgccccg gcagccttgg
     1441 gggagctcat ccgcctgctg gcgtccatgc ccgacgtgcg cgacatggac gtgcgcaata
     1501 ccgcgcgcca ggtctttgcc ctggacgagg ccgcccaggc ccaggtgcgg caggccgcgg
     1561 ggctttacct gtccaacgcc tacagtgccc gcagcgtgcg ctacaaggcc ctcgggcagg
     1621 attttcacgc ctttctcggg caggtctatg atctgttgat gtccgggctg atcggcgact
     1681 tcacgattcc gcgggacgac ccgctgcgcg ccgagatcct gctgcgcacg gtgcgaaccg
     1741 ctgcgggcga gttcaagtgg gccgccttcg attatcagtc cctgtcgccg gaagcctggc
     1801 ggcgggccat gagcgcctat cgcctggcgc tgtccgccgg ggtggccgac ctgccggtgc
     1861 ggctgcggga ggggcgcgag agccggacga cggtcagccg cgaattcgcg cgcctggtgg
     1921 ccatgcaatg cgccggcttc gagcagctgt cgccagaacg catcgaggcc gccgacaagc
     1981 tgatgcgctt tgtgcagcat gccctgcggc tgaccggcga accgctggaa ggcagtcaat
     2041 tttccgtgga tctggatggc ttcgagcggc cgcggcgtct cctcaatctg cccgaagcgg
     2101 ctggcacgct gcgcttcttc cggcctggtg acgcggtgcc ggtactgggc gatctggcgg
     2161 gatcggtgct gctgcccggg gggcatgcgg ctttcgccgg taccaatcca gtcgagatcg
     2221 gtgcggccat cgcccacctc aaccggcaat ggggcgcgcg tgcgcccatg cgccagtatc
     2281 ggcgtcatcc catgcgtggc gaggtgtccc tgtccaccgg gctgggtctg atccggtcgc
     2341 tcatctcggg tgaggctcag tttcggccgg cgcccgcatg gcatctggtg gatgcgagtc
     2401 gcgacggctt ctgccttgcg tcgcgcctgt tcgacggtga ggtgtgtcgt gtcggcacgc
     2461 tggtgggggc ctatctcggc gaatggggga cgtggaccct ggccgtggtg cgccgcgttc
     2521 agcacagtga gcggcagggc acatccatcg ggctgcagat cctcaccagc ggcccgcgcc
     2581 ccgcggcgct ggatgacggc agccgaagct ggctcggggt actctgcgac ccggtggtcc
     2641 gtgggcgcgc cctgcgcatc gtctgtgagc cgggtttcac cgggctgggc gggcgtctct
     2701 ttgtcaagca ggggccgcgc accgtcaagg cggacgtcga gcacctcgtg gcccgtgggc
     2761 cgggcttcga agtctggggc tgcaaggtcc gatagcgcgc gctgcgttga cacggtacag
     2821 gcgggctgct aaggttcgac gcatgtacgc ctcgcaacag accgctctct tctcccttct
     2881 ggcccgttcc gggctgggtc tgctgctgcc ctaacgggca cactttcttt ccccttccct
     2941 gttgtcccga atcctgcccc ttgtgggcac gttgcgtctc tgcacgcgtt ttctccggag
     3001 tcttgtcatg tccaccctga acaccttcct gctgtccctg cgactgcgac ccggttgcca
     3061 ggccacgagc ccgcgacagc ctggcagcct tgcgcccgct cctgaattcc atttccgacg
     3121 acagcccagg atacgacgat catgttgaag aacccgaaca ccaagtattc cccctttccc
     3181 ccggtcgccc ttgccgaccg ccaatggccg agccgcacga tcacccatcc gccggtgtgg
     3241 atgagcaccg atctccgcga tggcaaccag gccctcttcg aaccgatgaa cgcggcgcgc
     3301 aagttgcaga tgttccggca actcgtggcg atcggcttca aggaaatcga ggtcgccttt
     3361 ccctccgcgt cccagaccga cttcgacttc gtccgccagc tcatcgaggg cggcgagatc
     3421 ccggacgatg tcaccatcga ggtgctcagc caggcccgcg agcatctcat ccgccgcacc
     3481 atggaatccc tccgcggcgc ccgtcgggcc atcgtgcaca tctacaacgc cacctcgcgg
     3541 aatttccggg agactgtctt cggcatgagc cgggacgagg tgaaggccat ggcggtggac
     3601 agtgtccgcc tcgtcaagac gctcgccgcc gagcagccca caaccgaatg ggtgctcgag
     3661 tacagcccgg agaccttcac cgccaccgaa ctcgacttcg cccgtgaggt ctgcgatgcc
     3721 gtggtggccg cctgggatcc gcgtcccggt gagaaggtca tcctcaacct tccgtccact
     3781 gtggagatgg ccacgcccaa cgtctacgcg gaccagatcg agtggatgca tcgccatctc
     3841 gcgcggcgcg agcagatcat cctcagcgtg catcctcaca acgatcgggg cacggcggtg
     3901 gcggcggcgg agctcgccgt gctcgccggt gccgagcgtg tcgagggctg tctctttggc
     3961 aacggggagc gcacgggcaa cgtggatctt gtgaccctcg ccctcaacct ttacacccac
     4021 ggcatcgatc cggggctcga cttttcggac atcaacactg tcgtgcgggt ggccgaagcc
     4081 tgcacccagt tgcccgtcca tccgcgccat ccctacgcgg gcgatctcgt gttcacggcc
     4141 ttctcgggat cccaccagga tgccatcaag aagggcttgc agatccagca gccggacacg
     4201 ctctggcagg tgccctactt gccggtggat ccggcagacc tcgggcgtac gtacgactcg
     4261 gtgatccggg tgaacagcca gtccggcaag ggcggcgtcg cgcaccttct cgaatccgcc
     4321 tacggtctgg tcatgccccg gcgcatgcag gtggagttca gcggcgccgt acagcgcctc
     4381 accgacgaga ccgggcagga agtggatgcc catcagttgt ggcagctctt ctgccgcgaa
     4441 tacctcgatg ccgacgcgcc cgtccgctac ggcgatcacc acctctacga ggcggacggg
     4501 cgacagggca tccgaatcga ggcgcacatc gacgaccagg cacaggtgct gaagggcgag
     4561 ggcaacggac cggtggaagc cgccgtggcg gccttgcgcg tgccggtgcg ggtgcagagc
     4621 ttcgaggagc gctccatggg cgaaggggct gacgcggtgg cgatggccat cgtcgaggtg
     4681 gccatgccgg gggtcgccgg gacctgcttt ggggcggcga tgcaccgcaa tacggtgacg
     4741 gcctccgtgc gggccgttgt tgccgcggtc aatcgcatcc ttgcccgcca gggcggtggg
     4801 gcgaatcccg gtgcggttga ggcggttcag gcaggctgtg ctgcctgagc cgacaggctc
     4861 gccgcgtcag gcgggggctt cggcccccgt tttttgtgcc cggtggcggc gttcagaaac
     4921 ggatggccag aacgatctgg tgcacatcca gcccgtcgtt ggggagtcgg atgtcggcat
     4981 tggaatagtg cgaaaactgc cagcccaggg atgcccgccc gccgagggtc acgcccgcgc
     5041 ccacgtgttc ggaaaactgg aagtgggtcg agaaggtctt gtcgcccagg cggtcgtggc
     5101 tgaacagggc gccgccgagc cccgcctcca cgtaagcttg cagaccggcg ccgggatggg
     5161 tgacgcggag aacaccgagg atgccggcct cgtccaggct gtggggcgcg tcggctgtcc
     5221 cgctgtagcg gagacggctg aattccagtt ccggcttcaa cgctgccgtc catgggcccc
     5281 acatggaagt ccataaggtg ccgaagcgca gaccgatgcc gttgcgctcg aagcgcccgc
     5341 cgactgtgcc gtgcttgatg agcagctgca gatcgtcggc ctgggccggg ggtgtgagca
     5401 tcagggcaag gccgacgccg gccgccagcc cacgtagggc gacgcgccgg gagtgcgtaa
     5461 ggaggtgtgc gttcatgacc gtctcgcgga tcgggtggcg cttgggatgc cacgcaaggg
     5521 actggccttc ctgtccccgt gggaccgcac cgccggcatg gggttcagga tccggcgggc
     5581 acacatggcg acactggcag gcgccgccat gcgggtcgct cgcgcggggc ggtcagggat
     5641 cacaccgcag cgagggcctg gtccagatcg gcgaggatgt cgtcgatgtg ctcgatgccg
     5701 atagacaggc gcaccatgtc ctcggacacc ccggcgcggg ccatctcgtc cggcgagagc
     5761 tggcggtggg tggtggaggc cgggtggcag gcgaggctct tgctgtcgcc gatgttcacc
     5821 aggcgcgtga ccaactggag ggcgtcctgg aagcgggcgc ctgcggcctt gccgtcaccg
     5881 tcggcgctct tgacgccgaa gttgaggatg ccggaggcgc ggccgcccat gtatttctgg
     5941 acgagggcgt ggtccgggtg atcgggcagg ccggcgtaat tcacccacgc aaccttggca
     6001 tgccccttca ggaattcagc taccttgcgg gtgttcttgc agatgcggtc catgcgcagc
     6061 gtgagggttt cgatgccctg caggatcagg aagacgttga acgggctgat ggcggcgccc
     6121 atgttgcgca ggggcacgac ccgcgcgcga ccgatgtagg cggccgggcc gagggcttcg
     6181 gtgtagacca cgccgtggta ggagacgtcc ggctcgttga ggcggcggaa gcgggccttg
     6241 tgctcggccc acgggaactt gccgctgtcc acgatgaggc cgccgatgga gttgccgtga
     6301 ccgcccaggt atttggtgag ggaatgcacg acgatgtcgg cgccgtgctc gatggggcgg
     6361 cacaggtaag gcgagggcac cgtattgtcc acgatcagcg ggacgccatg ggcgtgggcg
     6421 atggcggcga gggcttcgaa gtcggtgatg ttgcccaggg ggttgccaac ggattcgcag
     6481 tagatggcct tggtgcgttc gtcgatgagg ggcgcgaagg agtccgggtc gcggtagtcg
     6541 gcaaagcgca cctggatgcc gatctggggc agggtgtggg cgaacaggtt gtaggtgccg
     6601 ccgtacaggg tggaggcgga gacgatgttg tcgccggcct cggcgatggt ctggatggcg
     6661 taggtgatgg ccgccatgcc cgaggccagg gccagcccgg cgatgccgcc ttccatggcc
     6721 gccacacggg cctccagcac ggcctgggtc gggttcatga tgcgggtgta gatgttgccc
     6781 gccaccttca ggtcgaacag gtcggcgccg tgctgggtgt cgtcgaacgc gtaggaggtg
     6841 gtctggtaga tcggcacggc gacggccttg gtggtggggt ccgggctgta gccggcgtgg
     6901 acggcgaggg tttcgatctt catgggtagc ggtctcctgt gtgtggtgtg attgtgtgtg
     6961 atcggaattc ggtgtgggga g
//
LOCUS       DAOQMM010000137         6957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_5880_length_6957_cov_55.609823, whole genome shotgun sequence.
ACCESSION   DAOQMM010000137 DAOQMM010000000
VERSION     DAOQMM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5880_length_6957_cov_55.609823"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLH85_15860"
     CDS             complement(<1..1387)
                     /locus_tag="PLH85_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNB66093.1"
                     /translation="MAAPEKEVTELPDPKEIAKTYAEVAQRASKLLADHMQKQVKKGI
                     ATPSDELGIAQAFMDMMGKLLANPYRLAQAQMNLVWDYFSLWQHSMMRFTGMGGGAPV
                     ATPAKDDKRFKDEEWQEHFLFDFIKQSYLITARHIHDTVSTVDGLDDQSQKKVNFYTR
                     QYIDALSPSNFALTNPEVFRETVKSHGQNLIKGLNNLLRDVEDGGGNLRVRMTDTSAF
                     ELGRNVATTPGKVVFQNEMMQLLQYTPSTPEVSKRPLLIVPPWINKFYILDLREKNSY
                     IKWCVDQGHTVFVISWVNPDERLAEKSFDSYVLEGTMAALDAIAEQTGAKEINAAGYC
                     LGGTLLATTLAYCAAKKDKRLASGTFFTTMTDFADPGELSVFIDEGQITSLEKKMFER
                     GYLEGSEMAGTFNMLRANDLIWSFVVNNYLMGKDPFPFDLLYWNSDSTRMPAKMHSFY
                     LRNMYLQNLLKE"
     gene            complement(1410..2441)
                     /locus_tag="PLH85_15865"
     CDS             complement(1410..2441)
                     /locus_tag="PLH85_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB66094.1"
                     /translation="MTQAPDAQLDYPCGEPPAPGDTREIAPGVHWIRMPLPFALDHIN
                     LWLIEDGDGLTLVDTGYGLEVTRDAWETILTRFARPVTRIVVTHCHPDHLGLAQWLAE
                     RSGAPVHLTLGEFLAAHALWHQLPGYNVSSMVAQFRRNGLDDARCAALEGRGNAYRKG
                     IPSLPESYCRLLHDDVLDIGGRAWRVISGYGHAPEHASLYCAEAGVLISGDMLLPRIS
                     TNVSVFAATPDDDPLARFLDSISAFKHLPGATLVLPSHGSPFRNIGLRVDQLVQHHAA
                     RCADLLGACDTPRSAGELLTTLFPRELDTHQVMFAMGEAIAHLNHLAHKREVCRVSGE
                     NGVVRFVKI"
     gene            2556..2993
                     /locus_tag="PLH85_15870"
     CDS             2556..2993
                     /locus_tag="PLH85_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNB66095.1"
                     /translation="MSQENTYTIAELAREFGITPRAIRFYEDEGLLSPGRVGRNRVYS
                     KRDRTRLRLILRGKRLGLSLAEIGELIGMYDGVRNSKPQLARFLVVLAERRAAIEQQR
                     EDIVAVLAEIGMLETQCRELLAENPAAAAEARAELAQQMDKNN"
     gene            3090..3560
                     /locus_tag="PLH85_15875"
     CDS             3090..3560
                     /locus_tag="PLH85_15875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB66096.1"
                     /translation="MHRPLPESFIPRDPAYAGRVRASFARQRAMALIGATLERVEPGC
                     TEIHLPTRDDLTQQHGFIHGGVIGMVADSAAGYAAATLVAADAEVLTVEYKLNLVAPA
                     EGECLVARGEVVKSGRSLSITRADVFAVRAGAWRLCAVMQQTIMNLHARPGPSS"
     gene            3604..4776
                     /locus_tag="PLH85_15880"
     CDS             3604..4776
                     /locus_tag="PLH85_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNB66097.1"
                     /translation="MNIPSLDFHLGETLTALRDTVQAFAAKEIAPRAAAVDRDNLFPA
                     DLWKKLGDLGLHGMTVEEDYGGSAMGYLAHIIALEEVSRASASVGLSYGAHSNLCINQ
                     IRRNGSEAQKRKYLPPLIAGDHVGALAMSEPNAGSDVVSMKLRAERKGERFVLNGSKM
                     WITNGGDADTLVVYAKTDPDAGPRGMTAFIVEKGMQGFSHGSHLDKLGMRGSNTFPLF
                     FDDVEVPEENVLGGVGNGARVLMSGLDYERAVLCGGPLGIMAACMDLVVPYLHERKQF
                     GQAIGEFQLMQGKVADLYTTWMATRAYVYAVGQACDRGDHARSLRKDAAGAILYSAEK
                     ATWMAGETIQTLGGVGYTNDYAAGRLWRDAKLYEIGAGTSEIRRMLIGRELFAETA"
     gene            4866..6008
                     /gene="can"
                     /locus_tag="PLH85_15885"
     CDS             4866..6008
                     /gene="can"
                     /locus_tag="PLH85_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNB66098.1"
                     /translation="MAVTLRVIGAEDRAEIEHVRQFFRTYAGWLGVDLDFQNFAAEME
                     TLPGAYAAPTGRLFFAELDGRPAGCVGIRAATEGVCEMKRLYVEPECRGTGVGRELAL
                     AGIKAAKAMGYRKIMLDTLPAMRIAVKLYRELGFKEAPAYYPTPVEGTMFLALDLENW
                     SEAEIYNENLFHLFDYNLAWARKMRQVDPGFFEKLSTLQTPEYLWIGCSDSRVPANEI
                     IGLLPGEVFVHRNVANVVVHTDLNCLSVIQFAVDVLKVKHIMVVGHYGCGGVRAALQR
                     DRVGLVDLWLRHVQDVHVKHYGRVDMLPPELRHDRLCELNALEQVVNVCQTVVVQDAW
                     KRGQRLTVHGWVYGLKDGLIRDLGINVSRRDDLVARYQAALEALEA"
     gene            6117..6557
                     /locus_tag="PLH85_15890"
     CDS             6117..6557
                     /locus_tag="PLH85_15890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB66099.1"
                     /translation="MAKAIRLRDDIDPATLTARGTGHLPGWYGLSVTELEPGRLRARM
                     TVRPEMLAPNGFLHAASVVALADTSAGYATVAHLPDGATSFTTLELKSNFFATVTEGE
                     LVCDATAVHLGRSTQVWDAEVRAADGARLALFRCTQMILWPKAA"
     gene            6572..>6957
                     /locus_tag="PLH85_15895"
     CDS             6572..>6957
                     /locus_tag="PLH85_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNB66100.1"
                     /translation="MTASLKAPLDFYFDFSSPYGYLAAEKIDALAAAHGRSVLWHPIL
                     LGVTFKATGLAPLPAIPLKGEYSLRDIARSARFLGVPYRKPTVFPIPTQHAARAFLWL
                     NDRDPQQARAFGLAAYRAYFTEDVNIS"
BASE COUNT         1138 a         2229 c         2362 g         1228 t
ORIGIN      
        1 gctccttgag gaggttctgc aggtacatgt tgcgcaggta gaagctgtgc atcttggccg
       61 gcatgcgggt ggaatcggag ttccagtaca gcaggtcgaa ggggaagggg tccttgccca
      121 tcaggtagtt gttgaccacg aaggaccaga tcaggtcgtt ggcccgcagc atgttgaagg
      181 tgccggccat ctcggagcct tccaggtaac cgcgctcgaa catcttcttc tcgaggctgg
      241 tgatctggcc ttcgtcgatg aacacggaca gttcgcccgg atcggcgaag tcggtcatgg
      301 tggtgaagaa ggtgccggag gcgaggcgct tgtccttctt ggcggcacag taggccaggg
      361 tggtggcgag cagggtgccg cccagacagt agccggcggc gttgatttcc ttggcgccgg
      421 tctgctcggc gatggcgtcg agggcggcca tggtgccttc gaggacatag ctgtcgaagc
      481 tcttctcggc caggcgctca tccgggttga cccaggagat cacgaacacg gtgtgaccct
      541 ggtcgacgca ccacttgatg taggagttct tctcgcgcag gtcgaggatg tagaacttgt
      601 tgatccaggg cgggacgatc agcagcgggc gcttggacac ctcgggcgtg ctcggggtgt
      661 attgcagcag ctgcatcatc tcgttctgga acaccacctt gcccggcgtg gtggccacgt
      721 tgcggcccag ttcgaaggcg gaggtgtcgg tcatgcgcac gcgcaggttg ccgccgccat
      781 cttccacgtc gcgcagcagg ttgttgagac ccttgatgag gttctggccg tggctcttga
      841 cggtctcgcg gaagacttcc gggttggtca gggcaaagtt ggacggcgac agggcgtcga
      901 tgtactggcg ggtgtagaag ttgaccttct tctgggactg gtcgtccagg ccatccacgg
      961 tggagacggt gtcgtggatg tggcgggcgg tgatgaggta ggactgcttg atgaagtcga
     1021 acaggaagtg ctcctgccat tcttcatcct tgaagcgctt gtcgtccttg gcgggcgtgg
     1081 ccaccggggc gccgccgccc atgccggtga agcgcatcat ggagtgctgc cacagggaga
     1141 agtagtccca caccaggttc atctgggcct gggccagacg ataggggttg gcgagcagct
     1201 tgcccatcat gtccatgaag gcctgggcga tgcccagttc gtcggacggg gtggcgatgc
     1261 ccttcttgac ctgcttctgc atgtggtcgg ccaggagctt ggaggcccgc tgtgccactt
     1321 cggcgtacgt cttggcgatt tctttcgggt ccggcaactc ggtcacttct ttttcgggcg
     1381 cagccatttc ttgtgctctc ctggttaatt cagatcttga cgaatcgaac gacaccattc
     1441 tcccctgaca ccctgcagac ctcccttttg tgtgcaaggt gattcaagtg ggcaatggct
     1501 tcccccatcg cgaacatcac ctggtgggtg tcgagctcac gggggaagag cgtggtcagc
     1561 agctcacccg cgctgcgcgg cgtatcgcag gcaccgagca ggtcggcgca gcgcgcggcg
     1621 tggtgctgaa cgagctggtc cacacgaagc ccgatattcc ggaacgggct accgtgggat
     1681 ggtagcacga gcgtggcgcc cggcaagtgc ttgaaagcgc tgatcgaatc gaggaagcgg
     1741 gcgagggggt catcgtccgg ggtggcggcg aagacgctca cgttggtgga gatgcgtggc
     1801 agcagcatgt cgccggagat caggacgccc gcttcggcac agtacaggct ggcgtgctcg
     1861 ggggcatggc cgtagccgga aatcacccgc caggcgcgcc cgccgatgtc cagtacgtcg
     1921 tcgtggagga gccgacagta ggactcgggc agggacggaa tgcccttgcg ataggcgttg
     1981 ccacgaccct ccagcgccgc gcagcgggcg tcgtcgaggc cgttgcggcg gaactgggcc
     2041 accatgctgc ttacgttgta accgggcaac tggtgccaca gggcgtgggc cgcgaggaat
     2101 tcaccgaggg tcaggtggac cggtgcgccg ctgcgctcgg cgagccactg ggcgagcccc
     2161 aggtgatccg ggtggcagtg ggtgacgacg atgcgcgtca ccggccgggc gaagcgggtc
     2221 aggatcgttt cccaggcgtc gcgggtcacc tccaggccgt agccggtgtc cacgagggtc
     2281 aggccgtcgc cgtcctcgat gagccacagg ttgatgtggt cgagggcgaa gggcaggggc
     2341 atgcggatcc agtgcacgcc gggggcgatt tcgcgggtgt cgccgggtgc gggcggttcg
     2401 ccgcaggggt agtcgagttg ggcgtcgggg gcttgggtca tggctgttcc ttgggttgaa
     2461 gcgccgcgtt gcccgcggtc gagaaatctg atttactgaa ccgacgcgcc attttgtcac
     2521 cgttgtgcaa cggcctcgtc gtcgtgcccg tcctcatgag ccaggaaaat acctatacca
     2581 tcgccgaact ggcccgtgaa ttcgggatca ccccccgcgc catccgtttc tatgaggacg
     2641 aagggctgct ttcgccgggc cgcgtcgggc gcaaccgggt gtattccaag cgtgatcgta
     2701 cgcgcctgcg cctgatcctg cggggcaagc gcctgggcct gtcgctggcc gaaatcggtg
     2761 agctgatcgg catgtacgac ggcgtgcgca attccaagcc gcagctggcg cgctttctgg
     2821 tggtgctggc cgaacgccgt gccgccatcg agcagcagcg cgaggacatc gttgccgtgc
     2881 tggcggagat cggcatgctc gagacccagt gccgcgaact cctcgccgaa aatcccgccg
     2941 cggccgccga agcccgcgcc gaactggccc agcagatgga caaaaacaat tgatgcccac
     3001 cccttgattt ttttatgggt tagtttacgt ttacgtaaac gtaatttcaa gcgagctgcc
     3061 cgcacgaggt ggccgactgg agacatccga tgcatcgtcc gctcccggaa tccttcatcc
     3121 cgcgcgaccc cgcctacgcc gggcgggtcc gcgccagctt cgcgcgacag cgcgccatgg
     3181 cgctgatcgg ggcgaccctc gagcgcgtgg agcccgggtg caccgagatc catctgccca
     3241 ctcgcgacga cctgacccag cagcacggct tcatccacgg cggcgtgatc ggcatggtgg
     3301 ccgattcggc ggccggttat gccgcggcga cgctggtggc ggccgacgcg gaggtgctga
     3361 ccgttgagta caagctcaat ctggtggcgc cggccgaggg ggagtgcctg gtcgcgcggg
     3421 gcgaggtcgt gaagtccggg cgcagcctga gcatcacccg cgccgacgtg ttcgccgtgc
     3481 gcgccggcgc ctggcgcttg tgcgccgtca tgcagcagac catcatgaac ctgcacgcgc
     3541 gtccgggccc gtcgtcctga gggcgcgggc ggacacgaca ctacaaaaac agaggagaca
     3601 tccatgaaca tccccagcct ggatttccat ctcggcgaga ccctcacggc cctgcgcgac
     3661 accgtgcagg cctttgcagc caaggaaatc gccccccgcg ccgccgcggt ggaccgggac
     3721 aacctgttcc cggccgatct gtggaagaag ctcggcgacc tgggcctgca cggcatgacc
     3781 gtcgaggagg actacggcgg ctcggccatg ggctatctgg cccacatcat cgccctggag
     3841 gaggtgtccc gcgcctccgc gtcggtgggg ctgtcctatg gcgctcattc caatctgtgc
     3901 atcaaccaga tccgccgcaa cggcagcgag gcccagaagc gcaagtacct gccgcccctg
     3961 atcgccggcg accacgtggg cgcgctggcc atgtccgaac ccaacgccgg ctccgacgtg
     4021 gtcagcatga agctgcgcgc cgagcgcaag ggcgagcgct tcgtcctcaa cggttccaaa
     4081 atgtggatca ccaacggcgg cgacgccgac actctggtgg tgtacgccaa gaccgatccg
     4141 gacgccggtc cgcggggcat gaccgccttc atcgtcgaga agggcatgca gggtttcagc
     4201 cacggctcgc acctggacaa gctgggcatg cgcgggtcca acactttccc gctgttcttc
     4261 gacgacgtgg aggtgcccga ggaaaacgtc ctggggggcg tcggcaacgg cgccagggtg
     4321 ctgatgagcg gcctcgacta cgagcgcgcg gtgttgtgcg gcgggccgct gggcatcatg
     4381 gcggcgtgca tggacctggt cgtgccctat cttcacgagc gcaagcagtt cggccaggcc
     4441 atcggtgaat tccagctcat gcagggcaag gtggccgatc tatacaccac ctggatggcg
     4501 acccgcgcct acgtgtacgc cgtcggccag gcctgcgacc gcggcgacca cgcccgcagc
     4561 ctgcgcaagg acgcggccgg cgcgatcctc tattccgccg agaaggccac ctggatggct
     4621 ggcgagacga ttcagaccct gggcggtgtc ggctacacca acgactacgc cgccgggcgc
     4681 ctgtggcgcg acgccaagct ctacgagatc ggtgccggca ccagcgagat ccgccgcatg
     4741 ctgatcgggc gcgagctgtt cgccgagacc gcctgaggga tgcttcggtg cacaacgggg
     4801 atgaatcgga gcgccgggac gggtatcgtg tcggattctt gcgtattcat cggtgagtgg
     4861 atcccatggc tgtgactctt cgcgtaatcg gtgccgagga ccgggccgaa atcgagcatg
     4921 tgcgccagtt tttccgcact tacgcgggct ggctgggcgt ggatctggat ttccagaact
     4981 ttgcggccga gatggagacc ctgcccggtg cctatgccgc accgaccggg cgcctgttct
     5041 tcgccgagct ggacggcagg cctgccggct gcgtgggcat tcgtgcggcg accgaaggcg
     5101 tctgcgagat gaagcgcctc tacgtggaac ccgagtgccg cggcaccggt gtcggtcgcg
     5161 aactggccct ggccggcatc aaggccgcca aggcgatggg ctatcgcaag atcatgctcg
     5221 acaccctgcc ggccatgcgc atcgcggtga agctgtaccg cgagctgggc ttcaaggagg
     5281 cacccgccta ctatccgacg ccggtggaag gcaccatgtt cctcgccctc gatctggaaa
     5341 actggagcga ggccgagatc tacaacgaga acctgttcca cctcttcgac tacaacctgg
     5401 cctgggcgcg caagatgcgc caggtggatc ccggcttctt cgagaagctg tccaccctgc
     5461 agacacccga atacctgtgg atcggttgtt ccgattcccg cgtgccggcc aacgagatca
     5521 tcggcctgct gccgggcgaa gtcttcgtcc accgcaacgt ggccaacgtg gtggtgcaca
     5581 ccgacctcaa ctgcctgtcg gtgatccagt tcgccgtgga tgtgctcaag gtcaagcaca
     5641 tcatggtggt cggacactac ggctgtggcg gcgtgcgggc ggccctgcag cgcgaccggg
     5701 tggggctggt ggatctgtgg ctgcgccacg tgcaggacgt gcacgtcaaa cactacggcc
     5761 gcgtcgacat gctgccgccc gagctgcgcc acgaccgcct gtgcgagctc aacgcgctgg
     5821 agcaggtggt caacgtgtgc cagaccgtcg tcgtgcagga cgcctggaag cgcggccagc
     5881 gcctcacggt gcatggctgg gtgtatggac tgaaggacgg gctgatccgc gatctgggga
     5941 tcaacgtcag ccgtcgtgac gatctggtgg cccgttatca ggccgccctg gaggccctcg
     6001 aagcctgaac gccgcggcct gctgcgcggc gaggacgatt cgaccccgga cacacggggg
     6061 accctcgcgg ttggaccgga tggccggtcc gccgccccga caaggagacg acgcagatgg
     6121 ccaaggcaat ccgcctgcgg gacgacatcg atcccgcaac cctcaccgcc cggggcaccg
     6181 ggcacctgcc cggctggtac gggctcagtg tcaccgagct tgagccgggc cgtctccgcg
     6241 cccgcatgac ggtgcgcccc gagatgctgg cgcccaacgg cttcctgcac gccgcctcgg
     6301 ttgttgccct ggccgacacc agtgccggct atgccaccgt cgcccacctg cccgacgggg
     6361 cgacgagctt caccaccctt gagctgaaga gcaacttctt cgccactgtc accgagggcg
     6421 agctggtctg cgacgcgacc gccgtgcatc tggggcgcag cacccaggtg tgggacgcgg
     6481 aagtccgcgc cgccgatggc gcccgcctgg ccctgttccg ctgcacccag atgatcctgt
     6541 ggcccaaggc cgcgtgacgc cggagaccgc catgaccgct tccctgaagg cccccctcga
     6601 cttctacttc gatttctcct cgccctacgg ctaccttgcc gccgagaaga tcgacgccct
     6661 ggccgccgcc cacggccgca gcgtgctgtg gcacccgatc ctgctgggcg tcaccttcaa
     6721 ggccaccggc ctcgcgccgc tgccggcgat ccccctcaag ggcgaatact ccctgcgcga
     6781 catcgcccgc tcggcgcgat ttctgggcgt gccttaccgc aagcccacgg ttttcccgat
     6841 tcccacccag cacgccgccc gtgccttcct gtggctcaac gaccgcgacc cgcagcaggc
     6901 ccgcgccttc ggcctggcgg cctaccgggc ctacttcacc gaggacgtga acatctc
//
LOCUS       DAOQMM010000138         6824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_6088_length_6824_cov_39.209337, whole genome shotgun sequence.
ACCESSION   DAOQMM010000138 DAOQMM010000000
VERSION     DAOQMM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_6824_cov_39.209337"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="PLH85_15900"
     CDS             <1..1500
                     /locus_tag="PLH85_15900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB66101.1"
                     /translation="MNAILGMTHLLRRGPTTPEQDDRLGKIIAAAQHLLSVIDDILDI
                     SKIEAGKLEIEETDLDLATVLHRVADLVRDRARSRQVNLYVELPDPLPGPLRGDPTRL
                     TQALLNYASNAVKFTERGRIVLRARVEDADADNVQVRFEVEDTGIGIRADALDRLFNA
                     FEQADGSTTRRYGGTGLGLAITRRLAHLMGGEVGVTSAHGTGSTFWLTARFRRGAPAT
                     EPVAPPRPHATTRRPPGETRILVCEDNPINQEVALTLLKDAGYRADLAVDGIDAVGKT
                     AALRYDAILMDMQMPEMDGLEATRRIRSLPGYRDIPIIAMTANAFGKDRQDCLAAGMN
                     DHLAKPVDPDALIATLDAWLNAAPGSPTTTTAPPATEPPVEAPLPDLSTLPGMDLDFG
                     LQVVRGNVRRYARLLHMLVEHHGHDATALRDALAAGRSSDAGRIAHSLKGAAGNLGAE
                     RIRDLATRLNDRLRAQQPPDALQGDLDALDAALGEFRTAMASIPGAKDD"
     gene            1731..3143
                     /locus_tag="PLH85_15905"
                     /pseudo
     CDS             1731..3143
                     /locus_tag="PLH85_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            3928..5325
                     /locus_tag="PLH85_15910"
     CDS             3928..5325
                     /locus_tag="PLH85_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HNB66102.1"
                     /translation="MFTRRLLAALVPAVLATSAHAGVTLIGTGTLSGPDMSGLTGALE
                     SGASASTLGGIGSGVAWAGGDTFLFTPDRGPNATAWNSAVDDTTSYVSRFQTVNLGLS
                     SAVFTSGSVTTLTPTLTDTTLLSSATPLNYGSAPALNSSGVNYFTGRSDAFGAGLSSN
                     PNNARLDPESIRVAQNGKSVFVSDEYGPYIYQFDRATGERIGVITLPSKFAVSNLSSQ
                     GATEISGNTSGRVANKGMEGLAITPDGKTLVGIMQSPLIQDGGTDGSTVRIVTIDIAT
                     GKVTHEYAYKLDNIGTVAKPKYPTVSEIVAINDHEFLVDERDGKGLGDGSNAAVKKLY
                     KIDLSGAADVSALSGAANLAPKAVGKTLFLDVVAALTSVGYTTATIPAKLEGVAFGPD
                     VLVSGVVEHTLIVANDNDFLDTVGGIANPNRVFVFGVTDDTLPSYQAQRIPEPATLAL
                     AALGLMGCMVGRRKG"
     gene            complement(5612..6109)
                     /locus_tag="PLH85_15915"
     CDS             complement(5612..6109)
                     /locus_tag="PLH85_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66103.1"
                     /translation="MPTLQEEGREKWIHEDNLLNHRLSWLGVTQGLLFAALGLIISQT
                     GTTPMTNANLSAGHASDLKAQLLAKLINGVPLLGLVSSLLILVGTLAATVAMAKIKKR
                     YELPEYGISLITTVFGWICAVGIPYIFCVAWLIIINGHWGSVFSVLILFVVIATWIRV
                     VKANA"
     gene            complement(6203..>6824)
                     /locus_tag="PLH85_15920"
     CDS             complement(6203..>6824)
                     /locus_tag="PLH85_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66104.1"
                     /translation="DVGRLGGRSVETLFQQFLSALVGGLVVYIFGIRKLSIELRNAFI
                     QKQMSEFYSPIAGCRKRIRAKSEVRGKVSSAASEAWAELCAPCAEAKQPMLNHEERYA
                     PYGKIIEYDNNQLREELIPLYRKMLDLFTEKYWLADEDTRAYYQEFLEFVEIWERYLA
                     EALPDGVLRKLGHTEENVLPFYEHVESKLSALQEEINANRFWKLRL"
BASE COUNT         1355 a         2609 c         1835 g         1025 t
ORIGIN      
        1 atgaacgcca tcctgggcat gacccacctg ctgcggcgcg gccccaccac gccggagcag
       61 gatgaccgcc tcggcaagat catcgccgcc gcccagcacc tgctgtcggt catcgacgac
      121 atcctcgaca tctccaagat cgaagccggc aagctggaga tcgaggagac cgacctggat
      181 ctggccaccg tgctgcaccg ggtggctgat ctggtccgcg accgcgcccg cagccgtcag
      241 gtcaatctct atgtggaact gcccgacccg ctgcccggcc ccctgcgcgg cgaccccaca
      301 cgcctcaccc aggccctcct caactacgcc agcaacgcgg tcaagttcac cgagcgcgga
      361 cgcatcgtcc tgcgcgcccg ggtcgaagat gccgatgccg acaatgtgca ggtgcgcttc
      421 gaagtcgagg acaccgggat cggcatccgc gccgacgccc tcgaccgcct cttcaacgcc
      481 ttcgagcagg ccgacggctc caccacccgg cgctacggcg gcaccggcct cggcctcgcc
      541 atcacccgcc gcctggccca cctgatgggc ggcgaggtcg gcgtcaccag cgcccatggc
      601 accggcagca ccttctggct caccgcccgc ttccgccgcg gcgcgcccgc caccgagccc
      661 gtcgccccgc cccgccccca cgccaccacc cgacgtcccc cgggcgagac gcgcatcctc
      721 gtgtgcgagg acaaccccat caatcaggaa gttgccctga cactcctcaa ggatgcgggc
      781 tatcgtgccg atctagcggt agacggcatc gacgccgtcg ggaagaccgc ggccctcagg
      841 tatgacgcca tcctgatgga catgcagatg ccggagatgg acggactgga agccacccgc
      901 cgcatccgca gcctgccggg gtaccgcgac attcccatca tcgccatgac cgccaacgct
      961 ttcggaaagg accgccagga ctgcctggcc gccggaatga atgaccacct cgccaagccc
     1021 gtcgatccgg acgcgctgat tgccaccctc gacgcctggc tcaacgccgc cccaggcagc
     1081 ccaacgacca ccaccgcgcc ccctgcgacc gaaccgcccg tcgaagcccc gctccccgac
     1141 ctgagcaccc tgcccggcat ggacctggac ttcggcctgc aggtcgtgcg cggcaacgtc
     1201 cggcgctacg cacggctgct ccacatgctg gtggagcacc acggccacga cgccaccgcc
     1261 ctgcgcgatg ccctcgccgc cggacgcagc agcgatgccg gccggatcgc ccacagcctt
     1321 aagggcgccg ccggcaacct gggcgccgag cgcatccgcg acctggccac gcgcctcaac
     1381 gaccgcctgc gcgcccagca gccgccggac gcgctgcagg gcgatctcga cgccctcgac
     1441 gccgccctgg gcgaattccg caccgccatg gccagcatcc ccggcgccaa agacgattga
     1501 atgcggcgcg gccgccacac tccgcgcacc gcgtcgaaca ccaaacacga caaaatccac
     1561 gcaaaactcc gcgtaattcc gtacaattcg gcaaccgtgc gcccggccaa tcagcgcacc
     1621 ggccttgatc cgttgatccg gtgggttcca gaccccgaaa accgcagcgt ccgccccgat
     1681 tggtatcgcc ctccccgggc gttcaggccg tcgcaggagc acacaattcc atgacccagc
     1741 cccagtccgc cgtgcaggcg ccgctggcgg tcagctttgc tgacctggcc ctctcagagc
     1801 ccatccagcg cgccattgcc gataccggct acaccgttcc caccccgatc caggtccagg
     1861 cgatccccca ggtcctcgcc ggcggcgacc tcatggccgc cgcccagacc ggcaccggca
     1921 agaccgccgg cttcacgctg ccgatcctgc atcgcctgtc cgaggcccac gcccacaacg
     1981 tccagaccgg ccgaccacgc tgcctgatcc tcacccccac gcgggaactc gccgcccagg
     2041 tcgaggaatc cgtacagacc tacggcaagc acctgcccct cacctccatg gtgatgttcg
     2101 gcggcgtcgc catcaatccg cagatcacca agctcaagaa acgcgtggac atcctcgtcg
     2161 ccacgcccgg ccggctcctc gaccactgca cacaaaagac cgtggacctc tccggcgtcg
     2221 agatcctggt cctcgacgaa gccgatcgca tgctcgacat gggcttcatc cgcgacatcc
     2281 gcaagatcct cgcgatgctg cccaagcagc gccagaacct gctcttctcg gccaccttct
     2341 ctgacgagat caaggccctc gccgacggtc tcctgcacaa tcccggcttc gtcgaggtgg
     2401 cgcggcgcaa caccgcctcc gagctggtgc gccagtccgt gcacctgtgc ccgcaaaagc
     2461 agaagcgcga actcctcgcc ttcctcatcc agaagcacca gtggttccag gtgctggtct
     2521 tcacccgcac caagcacggc gccaacaagc ttgccgaata cctgggcaag cacggcatcc
     2581 cctccgccgc catccacggc aacaagagcc agtccgcgcg cacccgcgcc ctcgccgact
     2641 tcaaggacgc caagctccag gtcctcgtcg ccaccgacat cgccgcccgc ggcctggaca
     2701 tcgaccaact gccccaggtg gtgaacttcg aacttcccaa tgtgcccgaa gactacgtcc
     2761 accgcatcgg ccgcaccggc cgcgccgggg ccgaaggcgc cgccgtgtcc ctggtggatc
     2821 aggaggaact acccctcctc aaggacatcg agcgcaccat gaagcgcctc atcgacaagg
     2881 tcgaggtgcc cgacttcgtg ccctccgcca cccccgacga aggcgacacc cgcccgcccc
     2941 gcgaaccccg tggcacccac accggcagcc gcggccgtca cgacaaaccg cgcagcgaac
     3001 atccccgcgc cgccgccccc cagggcgacg ccaaggccgc cagcggcgaa ggcaaaccgc
     3061 aacacggcca gaccggcggg caaggccagg gccagaaggc cggcaagccc agacacggca
     3121 agcccccgca gcagggcaag ggtcagagcc agggtcaagg gcaaggacaa caggggcagg
     3181 gcaacggcca gggcaaaccc cagcacggca aggaacaacg tcagggcaag cacggccacg
     3241 gcggccaggg aggacagggc agccagcccg gccacaaggc cccgtccgcc ccccagggca
     3301 agaagcacgg cgcccatggc ggcgctcatg gcggccccaa tcccaacaag gccccgcgcc
     3361 cccgcgaagg cggcaatgtg gacgacgact tcggcaaccg cgtggactac cagccccgcg
     3421 ccaagaccaa ctatcgcgac cccgaagccg ccctcaaccg gcgcatcagc ggcgaaaact
     3481 accgcgcctc cggcttcggc aagaacggcc cccgcggcaa ccccaaccgc ggcacgcccg
     3541 ccctgctcga agagcccgtg cgccagaccg tcaccctcac cggccgccgc gtcggcgagg
     3601 tgaagaagac cggcctgccc aaggacgtgg acggcaacaa gtaagccgcg cccccgtctc
     3661 accacgcccg gccgcagtct tggccgggcg tgtgtccatc tgtaccccct tcgcgcacca
     3721 ttccgcgagc cttcgcgcct ccgcccgacg ctgcgccgcc cggacacggg tgccgcgccc
     3781 acccgcggca cgcaccaccg ccccggcgca cattctccgc ggcgcctcgc caagcccagc
     3841 gccttcccct gcatcgctcc gcaaaaacgt taatttctgc gcaacatggc ctgcgtaggc
     3901 tgacgccttt ttccatcagg agtcgccatg ttcacccgcc gactgttggc cgccctcgtg
     3961 ccggccgtgc tcgccacctc cgcccacgcg ggcgtcaccc tcatcggcac cggcaccctc
     4021 tcgggccccg acatgtccgg cctcaccggc gcgctggaaa gcggcgcctc cgcctccacc
     4081 ctcggcggca tcggctccgg cgtcgcctgg gccggcggcg acaccttcct gttcaccccg
     4141 gaccgcggcc ccaacgccac cgcctggaac agcgccgtgg acgacaccac ctcctacgtc
     4201 tcccgcttcc agaccgtcaa tctcggcctc agcagcgccg tcttcacctc cggcagcgtc
     4261 accaccctga cgcccaccct caccgacacc accctgctgt ccagcgccac gcccctcaac
     4321 tacggcagcg ccccggccct caacagcagc ggcgtcaact acttcaccgg ccgctccgac
     4381 gccttcggcg ccggcctctc cagcaacccc aacaacgccc gcctcgaccc ggaaagcatc
     4441 cgcgtcgccc agaacggcaa gagcgtcttc gtctccgacg agtacggccc ctacatctac
     4501 cagttcgacc gcgccaccgg cgaacgcatc ggcgtcatca ccctcccgtc caagttcgcc
     4561 gtttccaacc tgagcagcca gggcgccacc gaaatctccg gcaacaccag cggtcgcgtc
     4621 gccaacaagg gcatggaagg cctcgccatc accccggacg gcaagaccct cgtcggcatc
     4681 atgcaaagcc ccctcatcca ggacggcggc accgacggca gcaccgtgcg catcgtcacc
     4741 atcgacatcg ccaccggcaa agtcacccac gaatacgcct acaagctcga caacatcggc
     4801 accgtcgcca agcccaagta ccccaccgtc agcgaaatcg tcgccatcaa cgaccacgaa
     4861 ttcctggtgg acgagcgcga cggcaagggc ctcggcgacg gctccaacgc cgccgtcaaa
     4921 aagctctaca agatcgacct gagcggcgcc gccgacgtca gcgccctcag cggcgccgcc
     4981 aacctcgccc ccaaggccgt cggcaaaacc ctcttcctcg acgtcgtcgc cgccctcacc
     5041 agcgtcggct acaccaccgc caccatcccc gccaagctcg aaggcgtcgc cttcggtccg
     5101 gacgtcctcg tcagcggcgt cgtggagcac accctcatcg tcgccaacga caacgacttc
     5161 ctggacaccg tcggcggcat cgccaacccc aaccgcgtct tcgtcttcgg cgtcaccgac
     5221 gacaccctgc ccagctacca ggcccaacgc atccccgaac ccgccaccct cgccctcgcc
     5281 gcgctggggc tgatgggatg catggtgggg cgtcgcaagg gttgattgat gtaggcgtga
     5341 tgtatgggaa acgggccgga caggggactg tccggcccgt ttttatttga gcgcccggat
     5401 gaagctacaa ccggatcaag cgctgcgggg aaaggtcgac ccgtctgaat tcactgcatg
     5461 gaaagtgtcg ggttacgctg cgctaacccg acctactggg ctgacgaaca ctttgaacat
     5521 gatagatacg gatccttaat gcgatgccta acgatcaagc tcagtcgatt gaaaaatgcg
     5581 cagcgttttg aaggtctgct gcagcgtgta gttatgcatt tgcctttacc acacgaatcc
     5641 atgtggcgat cactacaaat aagatcagaa cggaaaaaac ggaaccccaa tgaccattaa
     5701 ttataattaa ccatgccaca caaaagatat acggaatccc gacggcacaa atccaaccaa
     5761 aaaccgtcgt aataagtgat attccatact ccggcaattc ataccgcttc ttgatcttcg
     5821 ccattgctac cgttgctgct aacgtcccaa ccagaatcaa gagactcgat acgaggccca
     5881 atagaggcac gccatttatg agcttagcga gcaattgcgc cttaagatcg cttgcatgtc
     5941 cagcggataa gtttgcattc gtcatcggcg ttgtccccgt ctgactaatg atcaacccaa
     6001 gtgctgcaaa aagcagcccc tgggtcaccc caagccaaga gagacgatgg ttcaacaagt
     6061 tatcttcgtg aatccacttt tcgcgtcctt cttcttgcaa agttggcatc acatagttcc
     6121 tattggggtg cataacgttt aaattgagcg gcctgcgcgg cttttcgcgc aggccgctcg
     6181 aatgatgggt tgggcgcgtt tgtcataggc gcagtttcca gaacctattt gcattgattt
     6241 cttcttggag cgcagagagc ttgctttcga catgctcgta gaaggggagc acgttttctt
     6301 cggtgtgccc aagttttcgc aagacaccat cgggcaatgc ctctgcgagg taacgctccc
     6361 aaatttcgac aaactcaaga aactcttggt aataggcgcg agtgtcttcg tcggcgagcc
     6421 agtatttttc agtgaagagg tcgagcattt tgcgatagag gggtatcagc tcttcacgca
     6481 gttggttgtt gtcgtactca atgatctttc cgtacggggc atatcgctct tcgtgattga
     6541 gcatgggttg cttggcctcc gcacacggtg cacatagctc cgcccaggct tcactggccg
     6601 cagaagatac cttcccgcgc acctcgctct ttgctcttat gcgtttgcgg cagccggcaa
     6661 tcggcgagta gaactcggac atttgctttt gaataaaggc gttccgaagt tcaatggata
     6721 gcttgcgaat gccgaaaata tagactacca agccgcccac gagcgcgctg agaaattgct
     6781 gaaacagtgt ttccacgcta cgacctccta agcgcccaac gtcg
//
LOCUS       DAOQMM010000139         6536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_6583_length_6536_cov_50.047524, whole genome shotgun sequence.
ACCESSION   DAOQMM010000139 DAOQMM010000000
VERSION     DAOQMM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6583_length_6536_cov_50.047524"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLH85_15925"
     CDS             <1..648
                     /locus_tag="PLH85_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB66105.1"
                     /translation="RRVVVAINVKDRDLGGFVAELQKTVGAQVQLPDGYYLEWGGQFQ
                     NLERAMGHLTIIIPVTVGAIFFLLFLLFGSLKLASLIILVLPFASVGGVIGLLVTGEY
                     LSVPASVGFIALWGIAVLNGVVLVSYIRHLRADGMPPAEAVVHGAVMRFRPVMMTATV
                     ALLALVPFLFASGPGAEVQRPLAIVVIGGLITSTLLTLVVLPTLYKWFDDTPYEA"
     gene            706..1044
                     /locus_tag="PLH85_15930"
     CDS             706..1044
                     /locus_tag="PLH85_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNB66106.1"
                     /translation="MKEIRAVIRPQKLPRLREVLRAVPGFPGMTLSKAAGCGATVRHV
                     PASIREELTDFTEKVRVEIVAPDDLVDALVEAIVQVASTGQTGDGLVWVTSIEHAVFI
                     RKTVGPPEGT"
     gene            complement(1052..3064)
                     /locus_tag="PLH85_15935"
     CDS             complement(1052..3064)
                     /locus_tag="PLH85_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB66107.1"
                     /translation="MSLFRPPAGVLLALAGLVSGAAVHASPEESPFFEDMPTVLSASR
                     LPEPLHEAPGAVTVLDRDFIRATGYRDVARLLRLVPGMQVGQERGHAQWVTYHGLGSD
                     YPTEIQVLIDGRSVFAPSSFGGVNWSGLPLTVEEIERIEVVRGTNSNAYGANAFLGVV
                     NIITRHSRQDPGGHVEANAGTHRIGDLAAAWAGGDERIGVRLSAAALNDDGFGSLFDG
                     RHTGLVSLRSDLRASTRDELTLRAGFADQRRDLGYPDSVFGNNAQRTSTTHNGTLHLI
                     WRHTDGPDEEWMASLYRNHESGVDRWNASAPPAYPSVPLDRNVSSMRTNAEFQHRFAL
                     GPSTRLVWGLEGRRDETDADTLFAGLRPPGFELYRAFSNLAWHATPAWQINLGALLEK
                     SPDTAAQFIPRAFVNWQASATDTFRAGYARAWEQRNLFDRYGDVRAYDPVSRALLAWP
                     YMPNPAARVPKVDTLELGYLGRFRALDATVDVRLFNERITDFVIREVIDPPMPVALPL
                     RTSRFVNLPTPVVLRGVETQWRLRPARDTELLINHTLIDRRADDATVTKRTAPYVASL
                     TWLQQYGGGWSSTASLLRMGPLAGGYGFVPGCDYAARAYTTVDLRLARRFTRGGRQIE
                     VALNGINLGAPHQEIADRSEQCLPAHLGTPVNPASPMGWLSLAVAL"
     gene            complement(3201..4700)
                     /locus_tag="PLH85_15940"
     CDS             complement(3201..4700)
                     /locus_tag="PLH85_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNB66108.1"
                     /translation="MKSAVLRYSLAASAIAVALAGCLPEGAAISSSHAANATSATAPA
                     VPAAATTTAGRYGLPDFSALVEQVGPAVVNISVTQKRTLSNGDDFSGDPFDELLRRFG
                     IPGMPGMPGGNGGGRAPQQIQQGVGSGFIVSGDGYVLTNAHVVDGATEVTVRLTDKRE
                     FKAKVVGVDKRTDVALIKIDASGLPTVKTGDAEHARVGEWVVAVGSPFGFENTVTAGI
                     ISAKARRLPDENYVPFIQTDVAINPGNSGGPLFNLAGEVIGINSQIYSRSGGFMGISF
                     AIPIDVAMKVKDQLQRYGKVSRGRLGVSIQGLDPDLAQNFGLDKPAGALIANVENGSP
                     AEKAGLQSGDVVLGVNGQKVENSADLPRIIGEQKPGTSVRLSIWRDRKAREVSVTLGE
                     QLAEGAQPLGGFERKNEGASGKLGLTGRSLTAAEASRLGVAGGLLVEGVNGPAAKAGM
                     QPGDIILGLNNQPVTSVDQFRKLLEGAGNRFALLVQRGNSRIFLPIRMG"
     gene            5008..5925
                     /locus_tag="PLH85_15945"
     CDS             5008..5925
                     /locus_tag="PLH85_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNB66109.1"
                     /translation="MTLTDLRYLVALARERHFGHAAERCHVSQPTLSVAIKKLEDELA
                     VTLFERSAADVKITDIGARIVAQAERVLLEAGQVKEIATAGRNPLAGPLRLGVIYTVG
                     PYLLPDLIPRVHAAAPQMPLLIQENYTAKLVDALKRGELDVIVIALPFEEHGIVTQAV
                     YDEPFRVLIPADHAWSAEQTIAAQQLAEAQLLLLGAGNCFRDQVLEVCPMCRNGDGLQ
                     RTLEGSSLETIRHMVATGAGITVLPSTAADRIPATNTLLAVRPFADPEPSRRIALAWR
                     VTYPRSGAIDVIRAAILGSHLPGVRPVGH"
     gene            complement(5953..>6536)
                     /locus_tag="PLH85_15950"
     CDS             complement(5953..>6536)
                     /locus_tag="PLH85_15950"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNB66110.1"
                     /translation="PDGTAERRRALRSARIAARQALAPAQRSAATARIEAALDGLLAE
                     LAPRCLGFCWPWQGEVDLLPWMARWLAADPARQAALPVVVEAAAAMGFRRWTPDTPMT
                     ADRHGIPVPATDAPVQPDVLLVPLNVFDGAGFRLGYGGGYFDRTLAALRPAPTGIGVA
                     FELARADTVWPQPHDLPMDWIVTEAGAFRPRRG"
BASE COUNT          906 a         2205 c         2410 g         1015 t
ORIGIN      
        1 cgccgggtgg tggtggcgat caacgtgaag gaccgggacc tgggcggctt cgtggccgaa
       61 ctgcagaaga cggtgggcgc ccaggtgcag ctgccggacg gctattacct ggagtggggc
      121 ggccagttcc agaacctgga gcgggccatg gggcacctga ccatcatcat cccggtgacg
      181 gtcggcgcga tcttcttcct gctgttcctg ctcttcgggt cactcaagct ggccagcctg
      241 atcatcctgg tcctgccctt tgccagtgtc ggcggggtga tcggcctgct ggtgacgggg
      301 gaatacctgt cggtgccggc gtcggtgggc ttcatcgccc tgtgggggat cgcggtgctc
      361 aacggcgtgg tgctggtgag ctacatccgc cacctgcgcg ccgacggcat gccgcccgcc
      421 gaggcggtgg tgcacggggc ggtgatgcgc ttccgcccgg tgatgatgac cgccacggtc
      481 gccctgctgg cgctggtgcc cttcctgttt gccagcgggc cgggcgccga ggtgcagcgt
      541 ccgctggcca tcgtcgtgat cggcgggctg atcacctcga ccctgctcac cctggtggtg
      601 ctgccgaccc tctacaaatg gtttgacgat acgccctacg aggcgtgatg ttgccgcggc
      661 ggcgggctgt ggctgcgccg ccgcggggct tcctgaggat gtgacatgaa ggaaatacgt
      721 gctgtgatcc gcccgcagaa gctgccccgc ctgcgcgagg tgctgcgggc cgtgccgggc
      781 tttcccggca tgaccctgag caaggcggcg ggctgcgggg cgacggtgcg gcatgtgccc
      841 gcctccatcc gcgaggagct gaccgacttc accgaaaagg tgcgggtgga gatcgtcgcc
      901 cccgacgatc tggtggatgc gctggtggag gccatcgtgc aggtggccag caccggccag
      961 accggcgacg gcctggtgtg ggtgacgtca atcgagcacg cggtcttcat ccgcaagacc
     1021 gtcggtccgc cggaggggac gtgagggggg cttacagcgc caccgccagc gacagccagc
     1081 ccatcggcga ggccggattg accggcgtgc ccaggtgggc gggcaggcac tgctcggagc
     1141 ggtcggcgat ctcctggtgg ggcgcgccca ggttgatgcc gttgagggcg acctcgatct
     1201 gccggccgcc ccgggtgaag cggcgggcca ggcgcaggtc gacggtggtg taggcccgcg
     1261 cggcgtagtc gcagccgggc acgaagccat agccgccggc cagcggcccc atgcgcagca
     1321 ggctggccgt gcttgaccag ccgccaccgt actgctgcag ccaggtgagg ctggctacat
     1381 agggggcggt gcgtttggtg accgtggcgt cgtcggcgcg gcggtcgatg agggtgtgat
     1441 tgatcagcag ttcggtgtcc cgcgccgggc gcaggcgcca ctgggtttcc acgccgcgca
     1501 gcaccacggg cgtggggagg ttgacgaagc gcgaggtgcg caggggcagc gcgaccggca
     1561 tggggggatc gatcacctcg cggatgacga agtcggtgat gcgctcgttg aacaggcgca
     1621 cgtccaccgt ggcgtcgagg gcgcggaagc ggcccagata gcccagctcc agggtgtcca
     1681 ccttgggcac gcgggcggcg ggattgggca tgtagggcca cgccagcagg gcgcggctga
     1741 ccggatcgta ggcgcgcacg tcgccgtagc ggtcgaacag gttgcgctgc tcccaggcgc
     1801 gggcgtagcc ggcgcggaag gtgtcggtgg cgctcgcctg ccagttgacg aaggcccgcg
     1861 ggatgaactg ggccgcggtg tcggggctct tttcgagcaa cgcgcccagg ttgatctgcc
     1921 aggccggcgt ggcatgccag gccaggttgg aaaaggcccg gtacagctcg aagcccggcg
     1981 ggcgcaggcc ggcgaagagc gtgtccgcgt cggtctcgtc acgccggcct tccaggcccc
     2041 acaccagccg tgtggacggg ccgagggcaa agcggtgctg gaattccgcg ttggtgcgca
     2101 tgctgctcac gttgcggtcc aggggcacgc tggggtaggc gggcggtgcg ctggcgttcc
     2161 agcggtccac gcccgactcg tggttgcggt agaggctggc catccactcc tcgtcggggc
     2221 cgtcggtatg gcgccagatc aggtggaggg tgccgttgtg ggtggtgctg gtgcgctggg
     2281 cgttattgcc gaagacggag tccgggtagc ccaggtcgcg gcgctggtcg gcgaatccgg
     2341 cccgcagggt gagttcgtcg cgcgtgctcg cgcgcaggtc gctgcgcagg ctgacgaggc
     2401 ccgtgtggcg tccgtcgaaa aggctgccga agccgtcgtc gttcagggcg gcggcagaca
     2461 ggcgtacgcc gatgcgctcg tcgccgccgg cccacgcggc ggccaggtcg ccgatgcggt
     2521 gcgtgccggc gttcgcctcg acgtgtccgc ccggatcctg gcggctgtgg cgggtgatga
     2581 tgttcaccac gccgaggaag gcgttggccc cataggcgtt ggagttggtg ccacggacca
     2641 cctcgatgcg ctcgatctcc tcgacggtga ggggcaggcc ggaccagttc acgccgccga
     2701 aggagctggg ggcgaacacc gagcggccgt cgatgagcac ctggatctcg gtcggataat
     2761 cgctgcccag gccgtggtag gtgacccact gggcgtggcc gcgctcctgc ccgacctgca
     2821 tgccgggcac caggcgcagc aggcgggcca cgtcccggta gccggtggcg cggatgaagt
     2881 cccggtcgag gacggtgacg gcgccgggcg cctcgtgaag gggttctggc aggcgcgagg
     2941 ccgacagcac ggtcggcatg tcctcgaaga acggcgattc ttcgggggac gcgtgcacgg
     3001 cggcgccaga aacgagcccg gccagcgcca gcaggacgcc ggccggtggc cggaacaatg
     3061 acattacgga gtttttatcg gagtgtgccg gatgcgccct ctgtcatccg gcgggctccg
     3121 attatatcca gcccgcttcg gcttgttccg ttgtcatgac aacttggcaa actttgccgg
     3181 cctgcggaac ggtctccgcc tcatcccatg cggatgggca ggaagatccg gctgttgccc
     3241 cgctgcacca gcagcgcgaa acggttgccg gcgccttcca gcagcttgcg gaactggtcg
     3301 acgctggtca ccggctggtt gttgagcccc aggatgatgt caccgggctg catgccggcc
     3361 ttggcggcgg ggccgttgac gccttccacc agcaggccgc cggccacccc caggcggctc
     3421 gcttcggcgg ccgtgaggga gcgcccggtg aggcccagct tgccgctggc gccctcgttc
     3481 ttgcgctcga agccgcccag gggctgggcg ccctcggcga gctgttcacc cagcgtcacg
     3541 ctcacctcgc gggccttgcg gtcgcgccag atggacaggc gcacgctggt gccgggcttc
     3601 tgctcgccga tgatgcgcgg caggtcggcg gagttctcca ccttctggcc gtttacgccg
     3661 agcaccacgt caccgctctg gaggcccgcc ttttcggccg gcgagccgtt ttccacgttg
     3721 gcgatcaggg cgccggcggg cttgtcgagg ccgaagttct gggccagatc cgggtccagg
     3781 ccctggatgg acacgcccag gcgcccgcgg gagaccttgc cgtagcgctg gagctggtcc
     3841 ttgaccttca tcgccacgtc gatggggatc gcgaaggaga tgcccatgaa gccgccggag
     3901 cgggagtaga tctgggagtt gatgccgatc acctcgccgg cgaggttgaa caggggcccg
     3961 ccggaattgc ccgggttgat ggccacgtcg gtctggatga agggcacgta gttctcgtcc
     4021 ggcaggcggc gcgccttggc gctgatgatg ccggcggtca ccgtattctc gaagccgaag
     4081 ggcgagccca ccgccaccac ccactcgccc acgcgggcgt gctcggcgtc gccggtcttg
     4141 accgtgggca ggccgctggc gtcgatcttg atgagggcca cgtcggtgcg tttgtccaca
     4201 cccaccacct tggccttgaa ctcgcgcttg tcggtgaggc gcacggtcac ctcggtggcg
     4261 ccgtccacca cgtgggcgtt ggtcagcacg tagccgtcgc cgctcacgat gaagccggat
     4321 ccgacgccct gctggatctg ctggggcgcc cgcccgccgc cgttgccgcc gggcattccg
     4381 ggcatgccgg gaatcccgaa gcggcgcagg agctcgtcga aggggtcgcc ggaaaaatcg
     4441 tcgccattgc tcagggtgcg tttctgggtg acgctgatgt tcaccaccgc cgggccgacc
     4501 tgctccacca gcgccgagaa atccggcagc ccgtagcggc cggcggtcgt ggtcgcagcg
     4561 gcgggcaccg ccggcgccgt ggccgatgtg gcattggcgg cgtgggacga ggaaatggcg
     4621 gcgccctccg gcagacaccc ggcgagggcg acggcgatgg ccgaggcggc aaggctgtaa
     4681 cgcagcacgg cagatttcat gcacagactc ctgggttcga acgctgcatg gacgcccgcg
     4741 cggggccggg cgttcccgat acatacacgc ccttacaaat tgagggcggt tcgctgcaaa
     4801 tcaagggcga tgcgggcacg cgcccgggct ccggcggggc ggccgcttcc tctctggaaa
     4861 ggccccgggc cggtgcggcg cgggcgatct ccttgtcatg cacggcgccc acgggtcgcg
     4921 caagtgtcgc ggaaatccgc gtacgggtga acctttcggc cgcggcgttg tatagttgaa
     4981 acctatcgtc gttgcggagc cgcccccatg acgctgaccg atctgcgcta cctcgttgcc
     5041 ctggcccgcg agcgtcactt cgggcacgcg gccgaacgct gtcacgtgag ccagccgacc
     5101 ctctcggtgg ccatcaagaa gctcgaggac gagctggccg tcacgctgtt cgaacgcagc
     5161 gcggccgacg tcaagatcac cgacatcggc gcccgcatcg tcgcccaggc cgagcgcgtg
     5221 ctgctggagg ccgggcaggt caaggagatc gccaccgccg gccgcaatcc cctggccggt
     5281 ccgctgcgtc tgggcgtcat ctacacggtg gggccctacc tgctgcccga cctcatcccg
     5341 cgggtccatg cggccgcgcc gcagatgccc ctgctgatcc aggaaaacta caccgccaag
     5401 ctggtggacg ccctcaagcg cggcgaactg gacgtgatcg tcatcgccct gcccttcgag
     5461 gagcacggca tcgtcactca ggcggtgtac gacgaaccct tccgcgtgct gatccccgcc
     5521 gaccacgcct ggtcggccga gcagaccatc gccgcccagc agctcgccga ggcgcagctg
     5581 ctgctgctcg gcgccggcaa ctgcttccgc gatcaggtgc tggaggtgtg ccccatgtgc
     5641 cgcaacggcg acggcctgca gcgcaccctg gagggcagtt ccctcgaaac catccgccac
     5701 atggtggcca ccggcgccgg catcacggtg ctgcccagca ccgccgccga ccgcatcccg
     5761 gccaccaata ccctgctggc ggtgcgcccc tttgccgatc ccgaaccgtc ccggcgcatc
     5821 gccctcgcct ggcgcgtcac ctatccgcgc agcggcgcca tcgacgtgat ccgcgctgcc
     5881 atcctcggca gccacctgcc gggcgtgcgg ccggtcggac attgatccgt cgccccgccg
     5941 cgcggccgcc tttcagcccc gccgcgggcg gaaggcgccg gcctcggtga cgatccagtc
     6001 catgggcaga tcgtggggct ggggccacac ggtgtcggcc cgcgccagct cgaaggctac
     6061 gccgatgccc gtcggcgccg gccgcagggc ggccagggtg cggtcgaagt agccaccgcc
     6121 gtagcccagg cggaaacccg cgccatcgaa cacgttgagg ggcaccagca gcacgtccgg
     6181 ctgcaccggc gcgtccgtgg cgggcacggg aatgccgtgg cggtccgccg tcatcggggt
     6241 atccggcgtc cagcgccgga agcccatcgc ggcggccgcc tccaccacca ccggcagggc
     6301 cgcctggcgt gccgggtcgg ccgccagcca gcgggccatc cacggcagca ggtccacctc
     6361 gccctgccac ggccagcaaa agcccaggca gcgcggggcg agctcggcca gcaggccgtc
     6421 cagagccgct tcgatgcgcg ccgtggcggc gctccgctgg gccggggcca gggcctggcg
     6481 tgcggcgatg cgggcgctgc gcagggcgcg gcggcgctcg gcggtgccgt ctggcg
//
LOCUS       DAOQMM010000140         6509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_6626_length_6509_cov_46.889836, whole genome shotgun sequence.
ACCESSION   DAOQMM010000140 DAOQMM010000000
VERSION     DAOQMM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6626_length_6509_cov_46.889836"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PLH85_15955"
     CDS             complement(<1..1169)
                     /locus_tag="PLH85_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237778.1"
                     /note="catalyze the formation of cyanophycin which may act
                     to store excess nitrogen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNB66111.1"
                     /translation="MEVSRIRALRGPNLWSRHTSIEAIVSCADGECDLDGLPGYEARL
                     RNLFPEMGFLKPEGHHGTISLAHALELAALGLQSSAGCPVTFSRTSATIEKGVFQVVV
                     EYTEEKVGRLAFDLAVDLCTAALEERNFDLADALARLRELDEDVRLGPSTGSIVQAAV
                     ARGIPFRRLTEGSLVQFGWGSQQRRIQAAETDLTSAVAESIAQDKELTKNLLRAAGVP
                     VPFGRSVTDAEDAWAAAQEIGGLVVVKPRDGNQGKGVAVKLRSREEVMAAYTVAAEIS
                     DDVMVERYLPGHDYRLLVVGSKLIAAARRDPPLVIGDGVRTIRQLVEHVNSDPRRGVG
                     HATSLTKIRFDEIALATLTKQGYEADSVPPKGKRVILRNNANLSTGGTATDVTDDV"
     gene            complement(1246..3438)
                     /gene="cphA"
                     /locus_tag="PLH85_15960"
     CDS             complement(1246..3438)
                     /gene="cphA"
                     /locus_tag="PLH85_15960"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNB66112.1"
                     /translation="MTNKTIEFLNVFHLRGPNIWTYRAVLEAWVDIHDLEDCPSNVIP
                     GFYERLTALLPSLIEHRCSIGERGGFLKRLRDGTWPGHILEHVTLELQNLAGMSGGFG
                     KARETSDRGVYKVVIRAWQKDVSLAALHMGRDLLMAAIEGRPYDVDAAVRRLRDLVDK
                     YMLGPSTRCIVEAAADKDRRIPYIRLSEGNLVQLGYGARQRRIWTAETDRTSAIAESI
                     SREKDLTKSLLESCGVPVPEGRIVTSPEDAIEAAGDIGYPVVVKPSDGNHARGVFTNI
                     STPDEVAKAFPLSLAQGSEVIVERFVRGSEHRLLVVGGRMVAANKGDTVSVVGDGKHT
                     IDQLIDLQINSDPRRGDEHEFPLYRITLDRFPAAQMEVERQGYTGSSIPPDGQDVMIV
                     RTSNMAFDVTDQVHPDTAELACLAARIVGLDIAGVDLVCEDISLPLGAQKGAIVEVNA
                     GPGLLMHIKPGVGEPRPVGEAIVAHLFEHDADSRIPVVGVTGSRGKTPVARLVTHLLR
                     LSGQYTGLASSEGVFLDGRQVETPARTNWDAAERVLMNRSVDVAVFENGCRSILSDGT
                     VYDRCLVGVVTNVDAPLHYGEFYMDAPEKVWNVFRTQVDLVLPQGAAVLNAEDPAVVE
                     MAELSDGEVIFYGVDGQAPAIAQARSEGKRTVYVSDGAIVLATGDKAEAVARLNKIAM
                     LRNGAGERPGVLSNLLAAVAAAWALGVAPALIRAGVKPYDPNAPLRND"
     gene            3718..6063
                     /locus_tag="PLH85_15965"
     CDS             3718..6063
                     /locus_tag="PLH85_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB66113.1"
                     /translation="MTSQPALPPRASHNAGTDAYSDYLGPAVPADWRQRLDPTLEAGE
                     KVLASVVLDLDTALRFRGGLLAVTDRRLLARAQDDDTWQAWTYRDGLHLRHRDHAGVA
                     ALELMDDGGRLAGWRFTLAQDPAARNLADRFHAQLDCVLSGRAPTPPEPLTCPTCDAP
                     LPPGTDECPTCAREIHTPPSTWTLFRLWRFARPYKSQLLSGFILTLLATGATLIPPYL
                     TMPLMDEILIPFQNGTPIDTHRVTLILGGLLVAALFAWGLGWAKTYILALVSERIGAD
                     LRTTTYEHLQGLSLEYFGGKRTGDLMARIGAESDRICLFLSMNLLDFATDVLMIAMTA
                     AILVSINPWLALVTLVPLPFIAWLIHIVRDKLRYGFEQVDRVWSEVTSVLADTIPGIR
                     VVKAFAQEKREVARFREANARNLAVNDRVNKVWSLFSPTVTLLTEVGLLVVWGFGIWL
                     VSERDVTVGTLAAFLAYISRFYTRLDSMSRIVSVTQKAAAGAKRIFDILDHVSSVPEP
                     VNPQHLPKVTGAIEVRNVGFRYGNRTILRNVDLSIQPGEMIGLVGHSGSGKSTLVNLI
                     CRFYDVTEGSIRIEGVDLRAVPVAEYRQNIGLVLQEPFLFFGTIADNIAYGKPDATRE
                     EIIAAARAAHAHDFIMRLPFGYDSLVGERGQALSGGERQRISIARALLIDPRILILDE
                     ATSAVDTETEMEIQAALENLIRGRTTLAIAHRLSTLRKADRLVVLDRGQIVEIGNHDT
                     LMDKQGAYFRLYEAQTRKLDDDALGGGTPLPAAAHRQHAQA"
     gene            6085..>6509
                     /locus_tag="PLH85_15970"
     CDS             6085..>6509
                     /locus_tag="PLH85_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNB66114.1"
                     /translation="MSQPTLNLARNPFGQLVFTAADGTPHTVVPVRAFPIAAPAENIG
                     LINPEGEEVAWVDRLADLPDAPRRLIEDELASREFMPVIQRIRHCSSFATPSTWELDT
                     DRGPATLVLKGEEDIRRLKSPSLLIADSRGIHFLVRDRYA"
BASE COUNT          980 a         2408 c         2088 g         1033 t
ORIGIN      
        1 acgtcgtcgg tcacgtcggt ggcggtgccg ccggtggaca ggttggcgtt gttgcgcagg
       61 atcacccgct tgcccttggg cggcacggaa tccgcttcgt aaccctgctt ggtgagggtg
      121 gccagggcga tctcgtcgaa gcggatcttg gtgagggagg tggcatgacc gacgccgcgg
      181 cgcgggtcgc tgttcacgtg ctccaccagc tggcggatgg tgcgcacgcc gtcgccgatc
      241 accaggggcg gatcgcggcg ggccgcggcg atgagcttgc tccccaccac cagcaggcgg
      301 tagtcgtggc cgggcaggta gcgctcgacc atcacgtcat cgctgatctc ggcggccacg
      361 gtgtaggccg ccatcacctc ctcgcggctg cgcagcttga ccgccacgcc cttaccctgg
      421 ttgccgtcgc ggggcttgac caccaccagg ccgccgatct cctgggccgc ggcccaggca
      481 tcctcggcgt cggtcacgga gcggccgaag ggcaccggca cgccggcggc gcgcagcagg
      541 ttcttggtga gctccttgtc ctgggcgatg gattcagcca cggcgctggt caggtcggtc
      601 tcggcggcct ggatgcggcg ctgctggctg ccccagccga actgcaccag gctgccctcg
      661 gtgaggcggc ggaaggggat gccgcgcgcc accgcagcct gcacgatgga gccggtggac
      721 ggccccaggc gcacgtcctc gtccagctcc cgcaggcgcg ccaacgcgtc ggccagatcg
      781 aaattgcgtt cttccagcgc cgcggtgcac agatccaccg ccagatcgaa ggccaggcgg
      841 ccgacctttt cttcggtgta ctccaccacc acctggaaca cacccttctc gatggtggcg
      901 gaggtgcggc tgaaggtcac cggacagcca gcgctcgatt gcaggccgag ggcggcgagc
      961 tcgagggcgt gggccaggga gatggtgccg tgatggcctt ccggcttgag gaagcccatc
     1021 tcgggaaaca ggttgcgcag gcgggcttca tagcccggca gtccgtcgag gtcgcattcc
     1081 ccgtcggcgc acgacacgat ggcttcgatg gaggtgtgcc ggctccacag gttggggccg
     1141 cgcagggccc ggatgcggga aacttccatg gatgagagtc cttgtcttcg ttctcgcccg
     1201 gccgtcattg cggccggatc gtcatgattc gggcaggtgg aaggctcagt cgttgcgcag
     1261 cggcgcgttc gggtcgtagg gcttgacgcc cgcccggatc agcgccggcg cgacgcccag
     1321 cgcccaggcc gccgccaccg ccgccagcag attggagagc acacccgggc gctcgccggc
     1381 accgttgcgc agcatggcga tcttgttgag ccgcgccacc gcttcggcct tgtcgccggt
     1441 cgcgaggacg atggcgccgt cgctcacgta caccgtgcgc ttgccctcgc tgcgggcctg
     1501 ggcaatggcc ggcgcctggc cgtccacgcc gtagaagatc acctcgccat cggacagctc
     1561 ggccatctcg acgaccgccg ggtcctcggc gttgagcacc gcggcgccct ggggcaggac
     1621 caggtccacc tgggtgcgga agacgttcca caccttctcc ggcgcatcca tgtagaactc
     1681 gccgtaatgc agcggcgcgt cgacgttggt caccacgccc accaggcagc ggtcatacac
     1741 cgtgccgtcg gacaggatgc tgcggcagcc gttctcgaag accgcgacat ccacggagcg
     1801 gttcatcagc acccgctcgg cggcgtccca gttggtgcgc gccggcgtct ccacctggcg
     1861 gccgtcgagg aatacgcctt cgctgctcgc cagcccggtg tactggcccg acaggcgcag
     1921 cagatgggtg acgaggcgcg ccaccggcgt cttgccgcgg ctgccggtga cgcccaccac
     1981 ggggatgcgc gaatccgcgt cgtgctcgaa gaggtgggcc acgatggcct cacccaccgg
     2041 gcgcggttcg ccgacgccgg gcttgatgtg catgagcagg ccggggccgg cattgacctc
     2101 gacgatggca cccttctgcg cgcccagggg cagggagatg tcctcgcaga ccaggtccac
     2161 gccggcgatg tcgagcccga cgatgcgcgc cgccagacag gccagctcgg cggtgtcggg
     2221 atgcacctgg tcggtgacgt cgaaggccat gttgctggtg cgcacgatca tcacatcctg
     2281 gccgtccggc gggatggagc tgccggtgta gccctggcgc tccacttcca tctgggccgc
     2341 cgggaagcgg tcgagggtga tgcggtagag ggggaattcg tgctcgtcgc cgcgacgcgg
     2401 gtcggaattg atctggaggt cgatgagctg gtcgatggtg tgcttgccgt cgcccaccac
     2461 ggacaccgtg tcgcccttgt tggccgccac catgcgcccg cccaccacca gcaggcgatg
     2521 ctcgctgccg cggacgaagc gttcgacgat gacctcggag ccctgggcca gggacagggg
     2581 gaaggccttg gccacttcgt ccggggtgct gatgttggta aacacgccgc gggcatggtt
     2641 gccgtcgctg ggcttgacca ccaccgggta gccgatgtcg cccgccgcct cgatggcatc
     2701 ctccgggctg gtcacgatgc gcccttccgg caccggcacg ccgcaggatt ccagcaggct
     2761 cttggtgagg tccttctcgc gggaaatgct ctcggcgatg gcgctggtgc ggtcggtctc
     2821 ggccgtccag atgcgacgct ggcgggcgcc gtagcccaac tgcaccaggt tgccttccga
     2881 caggcggatg taggggatgc gccggtcctt gtcggcggcg gcctcgacga tgcagcgggt
     2941 gctggggccg agcatgtact tgtccaccag atcgcgcaga cggcgcacgg cggcgtccac
     3001 gtcgtaggga cgcccttcga tggccgccat cagcaggtcg cggcccatgt gcagggcggc
     3061 cagggacaca tccttctgcc acgcccggat gaccaccttg tagacgcccc ggtcggaggt
     3121 ctcgcgggcc ttgccgaagc cgccggacat gccggccagg ttctgcagct caagggtcac
     3181 gtgctccagg atgtggcccg gccaggtgcc atcccgcagg cgcttgagga agcccccccg
     3241 ctcgccgatg ctgcagcgat gctcgatcag ggacggcagc agggccgtca gtcgctcgta
     3301 gaagccgggg atcacgttgg aggggcagtc ctccaggtcg tggatgtcca cccaggcttc
     3361 gaggacggcg cggtaggtcc agatgttcgg cccccgcagg tggaacacgt tcaggaattc
     3421 gatcgttttg ttggtcatgg acagggcagg ataaggtcga cccgctcttc ggcgggatca
     3481 cggcagtgca tcggaaaccc gatgttccct taacgcgaaa ccttcgtggc ggattaccgg
     3541 cggaaaaaat aatcagtgta gagtgggtga cgtgaccgct tccagccgcc cgctccgtcc
     3601 gccgggtata cgccagtgta acgagtcagt aaacttcgcc cgcaattctg ccctacgccg
     3661 gccgattgct gtggccggat ttccgcgccc cgtcctgcgg ccccctcgcg ttccaagatg
     3721 acctcacagc ctgccctccc gccccgcgct tcccacaacg ccggcaccga cgcctattcc
     3781 gactacctcg gccccgccgt acccgccgac tggcgacagc ggctcgaccc gaccctggag
     3841 gcgggcgaga aggtgctcgc cagcgtcgtc ctcgatctgg acaccgcctt gcgcttccgc
     3901 ggtggcctgc tggccgtcac cgaccgccgc ctgctggccc gcgcccagga cgacgacacc
     3961 tggcaggcct ggacctaccg cgacggactc cacctgcgtc accgggatca cgccggcgtg
     4021 gccgccctcg aactgatgga cgacggcggc cgcctggcgg gctggcgctt caccctggcc
     4081 caggacccgg ccgcccgcaa tctggccgac cgcttccacg cccagctcga ctgcgtattg
     4141 tcgggccgtg cccccacgcc gccggagccc ctcacctgcc ccacctgcga cgcgcctctg
     4201 ccgccgggca ccgacgaatg ccccacctgc gcgcgggaga tccacacccc gccgtccacc
     4261 tggaccctgt tccgcctgtg gcgctttgcc cgaccctaca agagccagtt gctgtccggc
     4321 ttcatcctga ccctgctggc caccggcgcc accctgatcc cgccctatct gaccatgccg
     4381 ctgatggacg agatcctgat cccgttccag aacggcacgc ccatcgacac ccaccgggtc
     4441 acgctgatcc tcggtggcct gctggtggcg gccctcttcg cctggggcct gggctgggcc
     4501 aagacctaca tcctggcgct ggtgagcgag cgcatcggcg ccgacctgcg caccaccact
     4561 tacgaacacc tgcagggcct gtcgctggaa tacttcggtg gcaagcgcac cggcgacctg
     4621 atggcgcgca tcggcgccga gtccgaccgg atctgcctgt tcctgtccat gaacctgctg
     4681 gacttcgcca ccgacgtgct catgatcgcc atgaccgcgg cgatcctggt gtccatcaac
     4741 ccctggctgg ccctggtcac cctggtgccc ctgcccttca tcgcctggct catccacatc
     4801 gtgcgcgaca agctgcgcta cggcttcgag caggtggacc gggtctggtc cgaagtcacc
     4861 agcgtgctcg ccgacaccat ccccggcatc cgcgtggtca aggccttcgc ccaggagaag
     4921 cgtgaggtcg cgcgcttccg cgaggccaac gcccgcaacc tggcggtgaa cgaccgggtc
     4981 aacaaggtct ggtccctgtt ctcgcccacc gtcaccctgc tcaccgaggt gggcctgctg
     5041 gtggtgtggg gcttcggcat ctggctggtg tccgagcggg acgtcaccgt cggtacgctg
     5101 gccgccttcc tggcctacat ctcgcgcttc tacacccggc tcgactccat gagccgcatc
     5161 gtctccgtca cccagaaggc cgccgccggc gccaagcgca tcttcgacat cctcgaccac
     5221 gtctccagcg tgcccgaacc cgtcaatccc cagcacctgc ccaaggtcac cggcgccatc
     5281 gaagtgcgca acgtgggctt ccgctacggc aaccgcacca tcctgcgcaa cgtggacctg
     5341 tccatccagc ccggcgaaat gatcggcctg gtgggtcact ccggctccgg caagagcacc
     5401 ctggtgaacc tcatctgccg cttctacgac gtgaccgagg gctccatccg catcgaaggc
     5461 gtggacctgc gcgccgtgcc cgtggccgaa taccgccaga acatcggcct ggtgctccag
     5521 gagcccttcc tgttcttcgg caccatcgcc gacaacatcg cctacggcaa gcccgacgcc
     5581 acccgcgaag agatcatcgc cgccgcccgc gccgcccacg cccacgactt catcatgcgc
     5641 ctgcccttcg gctacgactc cctggtgggc gagcgcggcc aggccctgtc cggcggcgag
     5701 cgccagcgca tctccatcgc ccgcgccctg ctcatcgacc cgcgcatcct gatcctcgac
     5761 gaagccacct cggcggtgga taccgagacc gagatggaga tccaggccgc cctcgaaaac
     5821 ctcatccgcg gccgcaccac cctcgccatc gcccaccgcc tgtccaccct gcgcaaggcc
     5881 gaccgcctgg tggtgctgga ccgcggccag atcgtcgaga tcggcaacca cgacaccctg
     5941 atggacaagc agggcgccta cttccgcctc tacgaagccc agacccgcaa gctggacgac
     6001 gacgccctcg gcggcggcac cccgctgccc gccgccgccc accgccagca cgcccaggcc
     6061 tgacgcggcc gccaaggaat ccccatgtcc cagcccaccc tgaacctggc ccgcaacccc
     6121 ttcggccagc tcgtcttcac cgccgccgac ggcacgcccc acacggtcgt ccccgtgcgc
     6181 gccttcccca tcgccgcgcc cgccgaaaac atcggcctga tcaacccgga aggcgaggaa
     6241 gtggcctggg tggaccgcct cgccgacctg cccgacgccc cgcgccgcct catcgaagac
     6301 gagctcgcca gtcgcgaatt catgcccgtc atccagcgca tccgccactg ctccagcttc
     6361 gccaccccca gcacctggga actcgacacc gaccgcggcc ccgccaccct ggtgctcaag
     6421 ggcgaagaag acatccgccg cctcaagtcc ccgtccctgc tcatcgccga cagccgcggc
     6481 atccacttcc tggtacgcga ccgctacgc
//
LOCUS       DAOQMM010000141         6342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_6926_length_6342_cov_40.050422, whole genome shotgun sequence.
ACCESSION   DAOQMM010000141 DAOQMM010000000
VERSION     DAOQMM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6926_length_6342_cov_40.050422"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(458..1897)
                     /locus_tag="PLH85_15975"
     CDS             complement(458..1897)
                     /locus_tag="PLH85_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNB66115.1"
                     /translation="MSRVVVGPFNRVEGDLEVSLDFTDGRVSAAYVNSPLFRGFEQIL
                     VGRDPTDALAIVPRICGICSVSQSAAAAAALADLAGVQAPPNGRRVANLILAAENIAD
                     HFTHFYLFFMPDFARDDYRGRPWFDAAAARFKAVTGSAAADALPARAAFLQIMGCLAG
                     KWPHSMTLQAGGVARAPQTADLLRVFAALREFRTWLERHLFGDRLEHVAALDSVAALR
                     RWQATGPAAASDFGQFLRIADDLDLAALGRATDRFLSHGAYPRDAGWLFPRGVLDLPA
                     GVLQGFDPADVREDVTHAWLEPDAPRHPSEGDTQPVADKPGAYTWCKAPRWQGQVVEC
                     GALARQAVAGHPLIRELVAEAGGNVLSRVLARLLEIALVVPAMEAWLRELDTGPFRSP
                     VTLPTDGKACGLVEAARGALGHWLTVRNGHIVRYQIVAPTTWNFSPRDAAGTPGALEQ
                     ALVGLGEGEGLRVQHVVRSFDPCMVCTVH"
     gene            complement(1944..2939)
                     /locus_tag="PLH85_15980"
     CDS             complement(1944..2939)
                     /locus_tag="PLH85_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupU protein"
                     /protein_id="HNB66116.1"
                     /translation="MKLLWLQSGGCGGCTMSLLCSSARDLPGLLAGAGIELAWHPSLS
                     EATGDAVRALLAEFESGRTALDLLCVEGSLLTGPAGSGRFHMLSGTGRAMIDWVRSLA
                     ARARYTLAVGTCAAYGGITAAGLNPAGAVGLQYDGEQRGGLLGADYRSAAGLPVINVA
                     GCPTHPDWVTDTLIALALGRFAEADLDALGRPRFYADQLVHHGCTRNEFYEFKASAET
                     PGDLGCMMEHLGCKGTQAHADCNIRPWNGLGSCTNGGYPCISCTEPGFESPGHPFGLT
                     PKVAGIPIGLPSDMPKAWFVALAALSKSATPERVRRNSVVEAPVLAPATRRTRLK"
     gene            complement(2936..4468)
                     /locus_tag="PLH85_15985"
     CDS             complement(2936..4468)
                     /locus_tag="PLH85_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB66117.1"
                     /translation="MFRDADPPARAGARPMSRTGKSARLPGVLVVDDEVRSQEALRRT
                     LDEEFEVFTASSADEAWRIMERESVQVLLCDQRMPGESGVGFLKRVRAQWPDVVRLII
                     SGYTDSEDIIAGINEAGIYQYLLKPWQPDTLLMAIRNAAELFRLQAENQRLSLELRTS
                     GAVLRKRVEMKYERVKREFGLDGLARASDSPLNPVCELIGQVAPYDLSVLISGESGTG
                     KEMLARAIHYQSRRADRAFVVENCGALPDQLLEAELFGYKRGAFTGAYEDRIGLFQQA
                     DGGTLFLDEIGETSPAFQVKLLRVLQEGEFRPLGSPRPMTVDVRVIAATNRDLETEVR
                     EGRFREDLYYRLAIVPIHVPPLRERPMDIPLLAHRLLEVSCRALGKDCDGFTPEALAC
                     LAAYRWPGNVRELQNTILRMLALTAEPQLGAHLLSPRILQAPPDPGHADLAEVGFAGT
                     LQDRVEALEARVLKETLIRLRWNKTRAANELGLSRVGLRAKLVRYGLDSDGGDHSAGS
                     GE"
     gene            complement(4494..4796)
                     /locus_tag="PLH85_15990"
     CDS             complement(4494..4796)
                     /locus_tag="PLH85_15990"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNB66118.1"
                     /translation="MFIAMNRFRIAPGREAEFIEIWRTRESHLDSVPGFRQFHLLQGP
                     TTPEFTLFASHSVWDSAAAFEAWTRSEAFRKAHSGAGTRRDLYLGPPQFEGFEAVL"
     gene            complement(4809..5855)
                     /gene="hypE"
                     /locus_tag="PLH85_15995"
     CDS             complement(4809..5855)
                     /gene="hypE"
                     /locus_tag="PLH85_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNB66119.1"
                     /translation="MKTAYVRPLDLRHGTVDLSHGGGGRAMVQLIADVFAKALDNEWL
                     RQGDDGAVLPAPAPGERLVMATDAHVVSPLFFPGGDIGSLSVHGTINDVAMMGARPLY
                     LSASFILEEGFPLADLLRIVTNMGAAARAAGVPVVTGDTKVVEKGHGDGLYISTTGLG
                     ALPAGRRLSGAAARPGDAVLVSGTLGDHGMAILAHREHLAFESEIRSDSAALHTLAAD
                     VLAAAPDLRLLRDPTRGGLSATLNEIAQQSAVGIVIDEAAIPVDPQVAAACEFLGLDP
                     LHVANEGKLVAVCPPAQADAALAALRAHPLGARAARIGEVIADDHRFVQMKTALGGRR
                     MVDWLAGEQLPRIC"
     gene            complement(5852..>6342)
                     /gene="hypD"
                     /locus_tag="PLH85_16000"
     CDS             complement(5852..>6342)
                     /gene="hypD"
                     /locus_tag="PLH85_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNB66120.1"
                     /translation="KPVVIAGFEPLDVMQAILMLVRQVNDGRAEVENEFTRAVTPDGN
                     LKAQALMDAVFEIRPDFEWRGLGVIPHSALRLRPAFADFDAERRFGLDYRPVPDHKAC
                     ECGPILRGVKTPTDCKLFGTVCTPESPVGSCMVSSEGACAAHYTYGRFRDIPVLAEPV
                     AP"
BASE COUNT          955 a         2020 c         2475 g          892 t
ORIGIN      
        1 accatgcgag tttgatcact ccctatggtg aaagcctcac gatagcctgt gagccactga
       61 gcaggctatg tatctaaaca acaggctatc gtgagagaca tccaccctgg cgtgagaagc
      121 atccaccccg gtgtgagcgt ctcaccctcg ggtggatcaa aacgagcggg ctgtgtgagt
      181 gcgtcacagg ccggtgtgag aacgatcctc tggacggtga gaaggatcca ccccgtgcac
      241 aggggctcac ggcactgacg atctttgatc gcccgcaggg tgaagctcat caccacagcc
      301 gtcaggctct gcgctcacgg acgcagtctg tgtcacctgg gagggttatc gtcacgcacc
      361 tgtggcatgg attcatcgtg gcctgtgagg gcgtcggaaa tcgcctctcc gcctcatgac
      421 tcaaccaatc ttcacttcaa accaaagcgc cgacgcatca atgcacggta cagaccatgc
      481 aaggatcaaa actcctcacc acgtgctgca ccctcagccc ctccccctcg cccagaccta
      541 ccagagcctg ttccagggcg ccgggggtgc cggcggcgtc gcggggggag aagttccagg
      601 tggtgggggc gacgatctgg tagcggacga tgtggccgtt gcggacggtg agccagtggc
      661 cgagggcgcc gcgggcggct tcgacgaggc cgcaggcctt gccgtcggtg ggaagggtga
      721 cggggctgcg gaaggggccg gtgtcgagtt cgcgcagcca ggcttccatg gcgggcacga
      781 cgagggcgat ctccagcagg cgggcgagga cgcggctgag gacgttgccg ccggcttcgg
      841 cgacgagttc gcggatcagc ggatggccgg cgacggcctg gcgtgcgagg gcgccgcact
      901 ccaccacctg cccctgccag cggggggcct tgcaccaggt gtaggcgccg ggtttgtcgg
      961 cgacgggctg ggtgtcgcct tcggaagggt gacgcggggc gtcgggctcc agccaggcgt
     1021 gggtgacgtc ctcgcgcacg tcggcggggt cgaagccctg caggacgccg gcgggcaggt
     1081 ccagcacgcc gcgggggaag agccagccgg cgtcccgggg gtaggcgccg tggctgagga
     1141 agcggtcggt ggcgcggccg agggcggcca gatccaggtc gtcggcgatg cgcaggaact
     1201 gcccgaaatc gctggccgcg gcggggccgg tggcctgcca gcggcgcagg gcggccacgg
     1261 aatcgagggc ggcgacgtgc tcgaggcggt cgccgaagag atggcgctcc agccaggtgc
     1321 ggaattcccg cagggccgcg aagacccgca gcaggtcggc ggtctggggc gcgcgggcga
     1381 cgccgccggc ttgcagggtc atgctgtggg gccacttgcc ggcgaggcag cccatgatct
     1441 gcaggaaggc cgcccgcgcc ggcagggcgt cggccgcagc gctgccggtg acagccttga
     1501 agcgggccgc cgccgcgtcg aaccagggcc gcccgcggta gtcgtcgcgg gcgaaatccg
     1561 gcatgaagaa gaggtagaag tgggtgaagt ggtcggcgat gttctcggcg gcgaggatca
     1621 ggttggccac gcggcggccg ttgggcgggg cctgcacgcc ggccaggtcg gccagcgcgg
     1681 cggcggcggc ggcgctctgg gacacggagc agatgccgca gatgcgcggc acgatggcga
     1741 gggcgtcggt ggggtcgcgg ccgacgagga tctgctcgaa gccgcggaac aggggcgaat
     1801 tgacgtaggc ggcgctcacc cgcccgtcgg tgaagtcgag gctgacctcc aggtcgcctt
     1861 cgacgcggtt gaaggggccg acgacgacgc ggctcatggg cgtccgccga cagtctgcac
     1921 ggtgctgcgg ccagggcaga cgctcatttg aggcgcgtcc gtcgggtcgc cggggccagc
     1981 accggggctt cgaccaccga gttgcgccgc acccgctccg gcgtggcgga tttggagagg
     2041 gcggccaggg ccacgaacca ggccttgggc atgtcggagg gcagaccgat gggaatgccg
     2101 gccaccttgg gcgtcagccc gaagggatgg cccggcgatt caaagcccgg ctcggtgcag
     2161 ctgatgcacg ggtagccgcc gttggtgcac gaacccaggc cgttccatgg gcggatgttg
     2221 cagtcggcgt gggcctgggt gcccttgcag cccaggtgtt ccatcatgca gcccagatcg
     2281 ccgggggttt cggcgctggc cttgaactcg tagaactcgt tgcgggtgca gccgtgatgg
     2341 accagctggt ccgcgtagaa gcgcggccgg ccgagagcgt ccaggtcggc ctcggcgaag
     2401 cggccgaggg ccagggcgat gagggtgtcg gtgacccagt cgggatgggt cgggcagccc
     2461 gccacgttga tgacgggcag gccggcggcg gagcggtagt ccgcgccgag caggccgccg
     2521 cgctgttcgc catcgtattg caggcccacg gcgccggctg ggttgaggcc cgccgcggtg
     2581 atgccgccgt aggccgcgca ggtgcccacc gccagcgtgt agcgggcccg cgcggcgagg
     2641 ctgcgcaccc agtcgatcat ggcccggccg gtgccggaca gcatgtggaa gcgcccgctg
     2701 ccggccgggc cggtgagcag ggagccttcc acgcagagca ggtccagcgc cgtgcggccg
     2761 gattcgaact ccgccagcag ggcacgcacc gcgtcgccgg tggcttcgga gagggacgga
     2821 tgccaggcca gctcgatgcc ggcgccggcc agcaggcccg gcaggtcccg cgccgacgag
     2881 cacagcaggg acatggtgca gccgccgcag ccgccggact ggagccacaa cagcttcact
     2941 cgccgctccc cgcgctgtgg tcgccgccat cgctgtcgag cccgtagcgg accagcttgg
     3001 cacgcaagcc gacccgcgac aggcccagtt cgttggcggc gcgggtcttg ttccagcgca
     3061 ggcggatcag ggtttccttg aggacgcgcg cctccagcgc ctcgacccgg tcctgcaggg
     3121 tgccggcgaa acccacttcg gcaagatcgg catggcctgg atcgggcggc gcctggagga
     3181 tgcgcggcga cagcaggtgg gcgcccagtt gcggctcggc ggtcagggcc agcatgcgca
     3241 ggatggtgtt ctggagctcg cgcacattgc ccggccagcg gtaggcggcg aggcaggcca
     3301 gggcttccgg ggtgaagccg tcgcagtcct tgccgagggc gcggcagctc acctccagca
     3361 ggcggtgggc gaggagcggg atgtccatgg gccgctcgcg caggggtggc acgtggatgg
     3421 gcacgatggc gaggcggtag tacaggtctt cgcggaagcg cccttcgcgc acctcggttt
     3481 ccaggtcccg gttggtggcg gcgatgacgc gcacgtccac ggtcatgggc cgcgggctgc
     3541 cgagggggcg gaactcgccc tcctggagta cccgcagcag cttgacctgg aaggccggcg
     3601 aggtctcgcc gatctcgtcg aggaagagcg tgccgccgtc ggcctgctgg aacaggccga
     3661 tgcggtcctc gtaggcgccg gtgaaggcgc cgcgcttgta gccgaagagc tccgcctcca
     3721 gcagctggtc gggcagcgcg ccgcagttct ccacgacgaa ggcccggtcg gcacggcggc
     3781 tctggtagtg gatggcgcgg gcgagcatct ccttgccggt gccggactcg ccggagatga
     3841 gcacggagag gtcgtagggc gccacctggc cgatcagctc gcacaccggg ttgagggggc
     3901 tgtccgacgc gcgggcgagg ccgtccagac caaattcacg cttgacccgc tcgtacttca
     3961 tctcgacgcg cttgcgcagc accgcgccgc tggtgcgcag ctccaggctc aggcgctggt
     4021 tctcggcctg caggcggaag agttcggcgg cgttgcggat ggccatcagc agcgtgtcgg
     4081 gctgccaggg cttgaggagg tactggtaga tgccggcttc gttgatgccg gcgatgatgt
     4141 cctcggagtc cgtgtagccg gagatgatca ggcgcaccac gtcgggccac tgggcacgca
     4201 cgcgcttgag gaagccgacg ccggattcgc cgggcatgcg ctggtcgcac aggagcacct
     4261 gcacgctctc ccgctccatg atgcgccagg cctcgtcggc cgaggaggcg gtgaagacct
     4321 cgaattcctc gtcgagggtg cggcgcaggg cttcctggga gcggacctcg tcgtccacca
     4381 ccagcacgcc gggcaggcgg gcggacttgc cggtgcggct catcggacgg gccccggcgc
     4441 gggcgggcgg gtcggcatcg cggaacacgg tctcatcctc gcggtgtccg gccttacagc
     4501 accgcctcga agccctcgaa ctggggcggg ccgaggtaca ggtcacggcg cgtgccggcg
     4561 ccggaatggg ccttgcggaa ggcctcggag cgggtccagg cctcgaaggc ggcggccgag
     4621 tcccacacgg aatgggacgc gaagagggtg aactccgggg tggtggggcc ttgcagcagg
     4681 tggaactggc ggaagccggg cacgctgtcc aggtgggatt cgcgggtgcg ccagatctcg
     4741 atgaattcgg cctcccggcc gggggcgatg cggaagcggt tcatggcgat gaacatgggg
     4801 atctccggtc agcagatgcg gggcagttgt tcgccggcga gccagtccac catgcggcgg
     4861 ccgccgaggg cggtcttcat ctgcacgaag cggtggtcgt cggcgatcac ctcgccgatg
     4921 cgcgcggcgc gggcgccgag ggggtgggcg cgcagggcgg ccagggcggc gtcggcctgg
     4981 gccggcggac agacggcgac gagcttgcct tcgttggcca cgtgcagcgg gtcgaggccg
     5041 aggaattcgc aggcggcggc cacctggggg tccacgggga tcgccgcctc gtcgatgacg
     5101 atgccgacag cggactgctg ggcgatctcg ttgagggtgg cggacagccc gccgcgggtc
     5161 gggtcgcgca gcaggcgcag atccggtgcg gcagccagaa cgtcagcggc gagggtgtgc
     5221 agggcggcgc tgtcggagcg gatctcggac tcgaaggcca ggtgctcgcg gtgggcgagg
     5281 atggccatgc cgtggtcgcc gagggtgccg gagaccagca ccgcgtcgcc cggccgcgct
     5341 gcagcgccgg acaggcgccg ccccgccggc agcgcgccga ggccggtggt gctgatgtag
     5401 agcccgtcgc cgtggccttt ctcgaccacc ttggtgtcgc cggtgacgac gggcacgccg
     5461 gcggcacgcg cggctgcgcc catgttcgtg acgatgcgca gcaggtcggc cagcgggaag
     5521 ccttcttcga ggatgaagct ggcggacagg tacaggggcc gcgcgcccat catggccacg
     5581 tcgttgatgg tgccatgcac gctgaggctg ccaatgtcgc cgccgggaaa gaacaggggc
     5641 gacaccacgt gggcgtcggt ggccatcacc aggcgctcgc cgggcgcggg cgccggcagc
     5701 acggcaccgt cgtcaccctg gcgcagccat tcgttgtcga gggccttggc gaagacgtcg
     5761 gcgatgagct ggaccatggc acggccgccg ccgccgtgag acaggtcgac ggtgccgtgg
     5821 cgcagatcga gggggcggac gtaggcggtc ttcatggggc gacgggttcg gcaagcaccg
     5881 ggatatcccg gaaacggcca taggtgtagt gggcggcgca ggcgccttcg gaagacacca
     5941 tgcacgagcc cacgggcgac tcgggcgtgc acacggtgcc gaacagcttg cagtcggtcg
     6001 gcgtcttcac gccccgcagg atggggccgc actcgcaggc cttgtggtcg ggcaccggcc
     6061 ggtagtccag gccgaagcgc cgctccgcat cgaagtcggc aaaggccggg cgcaggcgca
     6121 gggcggaatg ggggatgacg cccaggccgc gccactcgaa atccgggcgg atctcgaaga
     6181 cggcatccat cagcgcctgg gccttgagat tgccgtccgg cgtgacggcg cgggtgaact
     6241 cgttctccac ctcggcgcgg ccgtcgttca cctggcggac cagcatcagg atggcctgca
     6301 tcacgtccag gggctcgaag ccggcgatga ccaccggctt gc
//
LOCUS       DAOQMM010000142         6117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_7332_length_6117_cov_47.507918, whole genome shotgun sequence.
ACCESSION   DAOQMM010000142 DAOQMM010000000
VERSION     DAOQMM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7332_length_6117_cov_47.507918"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLH85_16005"
     CDS             <1..495
                     /locus_tag="PLH85_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB66121.1"
                     /translation="GGNIRILSTPGKGTNVRFILPMTEAPAPRSDQPPARAAHHDAPR
                     GLVLLVEDEPEVRKVIREQLTELGYPVLEAANGIEAAAMLEAVDDIAVLVSDTVMPGL
                     GGRELAARARRLRPQLPILLITGYATQAPADGPDLPTLRKPFDRSDLAAALDALHRPE
                     PAAP"
     gene            492..1226
                     /locus_tag="PLH85_16010"
     CDS             492..1226
                     /locus_tag="PLH85_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB66122.1"
                     /translation="MNSPDKDSPLIFVVEDEADIARLICATLGEYGFRSESFATGRSL
                     LARVRRQAPELAILDLGLPDMDGLQVLRELQELHPCAALILTGRDGLTDRVLGLELGA
                     DDYLVKPFEPRELVARVRSILRRYQRAASAEPARHTGARFAGWTFDIGRLSLLHDDGR
                     ELSLSAAEAALLQTLLRRPNKILTREQLIGERDLDPFDRSIDVRISRLRRKLEDEPQD
                     PKIIKTVYGAGYLLCATVEWLGPGGA"
     gene            complement(1235..2899)
                     /locus_tag="PLH85_16015"
     CDS             complement(1235..2899)
                     /locus_tag="PLH85_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB66123.1"
                     /translation="MSIRRHILVLTSIAIVLLVAMAAAGGGALQRNLALIHTLTEEAV
                     PGVLIASDLDADLRQVQLDLLEVVRAPSGEVATPQRERLAASRTHMDALLQALALRSR
                     STVETGLLGQMRDSLVQYGQAMDETLALVDRGQRALAEANLFANVSEYERELHETLQT
                     FRVEKQRDKDASLQAVEVGMEETMIGLSGLGGLTLVALAVMAVRLHRRIVRPLRAMET
                     TMASIAGSLDFTQRVPVVRDDEIGQSVRAFNALLDTLQRSLGEMIAVIRNNTVATVEM
                     HQSAATVARIATQGSVSSRAIHAAVQSIREHILHIDQETRQAGTITEASAETAVRRSE
                     FVGATASRIAQLAARIDTASAKVFCLADEVMHIEGVVSEIRQIADQTNLLALNAAIEA
                     ARAGEAGRGFAVVADEVRKLAERSATATETINARIGAVLVASREATDLMTVVVGDMRE
                     STALAATASEAIADVRTSTGQVIDAVGEINRLVHIGHASSDEILSRMDHINGLLDQAT
                     CAAGHAQSAADAVRGISHAMAQIVDRFKIGEGSAVVAADAGAVQLF"
     gene            3164..4927
                     /locus_tag="PLH85_16020"
     CDS             3164..4927
                     /locus_tag="PLH85_16020"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNB66124.1"
                     /translation="MTATAPSARPFRCPFPRLLRLACALLLMVSTSDLLAQEDAAALE
                     FHRQQERERQLRQQQEPRPDVRLQDVSPSSARLPRDEHPCFPIRRIVLTGVEAERFQW
                     LADHTGRAEDGHPDPAIGACLGTGGVNLVMRRLQNALIERGYITTRILAAPQDLSTGT
                     LHLTVVPGRIHAVRFLPDASGQMPSRATHWNALPAAADDLLNLRDIEQALENFKRLPS
                     VEADLQIAPSEDAAATPGDSDLLITWQQTFPFRLTLSADDSGTQASGRHQGAATVAFD
                     NWWTLNDLFYITHSQSLFTDGTVRGTRGYTVHYSLPLGYSLLALTTSHNTYHQTVAGL
                     NGAVRYDGVNDTGDLRLSHILHRTAAGKTHVFLRGWQRASSNFTDDEEVLVQRRRMGG
                     WEAGAGHRAFIGQATLEGTVAYRRGTGAFDALPAPEEARGAMAEGTARPRLWTADLQF
                     NQPFRVGGEAFRYGTSLRAQWNGTPLVPQDRFAIGGRYTVRGFDGELVLAAERGWFVR
                     NDLGWQIGGQELYLGLDHGAVAGRSAAQLLGRTLTGAVIGLRGGGSALSYDLFTGTPV
                     ARPEGFQAARTVAGFSITWTL"
     gene            4973..>6117
                     /locus_tag="PLH85_16025"
     CDS             4973..>6117
                     /locus_tag="PLH85_16025"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNB66125.1"
                     /translation="MNRLCYRIVFNRLRGMLMAVAECACARSRSSGQGRRPVPQQPPP
                     PASVAARLRPTVLRCWLAFGLVLPALPAPAQIVAYKNPPAGQTPTVLAAPNGVPLVNI
                     RTPNAAGLSHNAYSRFDVPPQGTLLNNSPDAVQTQLGGWVQGNPWLATGSARIILNEV
                     FSNDPSLLRGYIEVAGQQAQVVVANPSGVTCTGCGFIHAHRVTLTTGTPRVAADGRLD
                     AYRVTGGTVLIDGNGLDTSSSDFTEIIARAVRVNAGLWARQLHVAAGTSLVDAATLDT
                     TPLASPTPAPALAIDVASLGGMYARKITLVGTEAGVGVRSAGTLGASIGEFVISAAGH
                     LENTGTLTSADALRVTTTGLRNSGTFYAQGEARVSTTGVLENDGLIAS"
BASE COUNT          968 a         2409 c         1838 g          902 t
ORIGIN      
        1 ggcggcaaca tccgcatcct cagcacgccg ggcaagggca ccaacgtgcg cttcatcctg
       61 cccatgaccg aggcgcccgc cccgcgcagc gaccagcccc ccgcccgcgc cgcccaccat
      121 gacgcgccgc gcggactggt cctcctcgtc gaggatgaac ccgaggtgcg caaggtcatc
      181 cgcgaacagc tcaccgaact gggctacccg gtgctggaag ccgccaacgg gatcgaagcc
      241 gccgccatgc tggaagccgt ggacgacatc gccgtgctgg tctccgacac cgtgatgccg
      301 ggcctcggcg ggcgcgaact cgccgcccgg gcgcgacgcc tgcgcccgca gctgcccatc
      361 ctcctgatca ccggttacgc cacccaggcc cccgccgacg gccccgacct gccgaccctg
      421 cgcaagccct tcgaccggtc ggacctggcc gccgccctcg acgccctgca tcgcccggag
      481 cccgccgccc catgaacagc cccgacaagg acagccccct catcttcgtc gtcgaagacg
      541 aagccgacat cgcccgcctc atctgcgcca ccctcggcga gtacggtttt cgcagcgaat
      601 ccttcgccac cggccgcagc ctcctcgccc gtgtgcgccg ccaggcgccc gagctggcca
      661 tcctcgacct gggcctgccg gacatggacg gcctgcaggt gctgcgcgag ttgcaggaac
      721 tccacccctg cgcagccctc atcctgaccg gccgcgacgg cctcaccgac cgggtactgg
      781 gcctcgaact gggcgccgac gactacctcg tcaagccctt cgaaccgcgc gagctggtgg
      841 cccgcgtgcg ctccatcctg cgccgctacc agcgcgccgc cagcgccgaa ccggcccgcc
      901 acacgggcgc ccgcttcgcc ggctggacct tcgacatcgg ccgcctcagc ctgctccacg
      961 acgacggccg cgaactgtcc ctgtccgccg ccgaagccgc cctgctgcag accctgctgc
     1021 gccgccccaa caagatcctc acccgcgaac agctcatcgg cgaacgcgac ctggacccct
     1081 ttgaccgcag catcgacgtg cgcatctcgc gcctgcgccg caagctggag gacgagcccc
     1141 aggacccgaa gatcatcaag accgtctatg gggccggcta tctgctgtgc gccacggtgg
     1201 agtggctggg gcccggcggc gcctgaaccc gccttcagaa cagctgcacc gcccctgcat
     1261 ccgcagccac caccgcagag ccttcgccaa tcttgaagcg atcgacgatc tgcgccatgg
     1321 cgtgggagat gccccgcacc gcatccgctg ccgactgggc gtgcccggcg gcgcaggtgg
     1381 cctggtcgag caggccattg atgtggtcca tccggctcag gatctcatcg ctcgacgcgt
     1441 gcccgatgtg caccagccgg ttgatctcgc ccacggcgtc gatgacctgg ccggtggacg
     1501 tgcgcacatc ggcaatggcc tcgctcgccg tcgccgccag cgccgtgctc tcgcgcatgt
     1561 cgcccaccac gaccgtcatc aggtcggttg cctcccgcga cgcgaccagt accgctccga
     1621 tgcgcgcgtt gatcgtctcg gtggcggtgg cggagcgctc cgccagcttg cgcacctcgt
     1681 cggccaccac ggcaaagccg cgcccggcct cgcccgcgcg ggccgcctcg atcgccgcgt
     1741 tgagggccag gaggttggtc tgatcggcga tctggcggat ctccgacacc acgccctcga
     1801 tgtgcatcac ctcgtcggcg aggcagaaca ccttcgccga cgccgtgtcg atgcgcgccg
     1861 ccagctgggc gatgcggctc gccgtagcgc cgacgaactc gctgcgccgc accgccgtct
     1921 cggctgaggc ctcggtgatc gtgcccgcct ggcgggtctc ctggtcgatg tgcaggatgt
     1981 gctcgcggat cgactgcacg gccgcgtgga tggcccgcga cgaaacgctc ccctgggtcg
     2041 cgatccgggc caccgtcgcc gccgactggt gcatctccac ggtggccacc gtgttgttgc
     2101 ggatcaccgc gatcatctca cccaggctgc gctgcagggt gtccagcaac gcattgaagg
     2161 cccgcaccga ctgcccgatc tcgtcatcgc ggaccaccgg cacgcgctgc gtgaaatcca
     2221 gcgagccggc aatcgacgcc atcgtcgtct ccatcgcccg caggggccgc acgatgcgcc
     2281 ggtgcaggcg caccgccatg accgccagtg ccaccagggt cagtccgccc agcccactca
     2341 ggccgatcat cgtctcctcc atgccgacct ccacggcctg gagcgaggcg tccttgtcac
     2401 gttgcttttc cacgcggaag gtctgcagcg tctcgtgcag ctcgcgctcg tattccgaca
     2461 cgttggcaaa gagattcgcc tccgccagcg cgcgctgtcc ccgatccacc agggcgaggg
     2521 tctcgtccat cgcctgaccg tactgcacca ggctgtcccg catctgcccc agcaggccgg
     2581 tctccaccgt cgaacgggag cggagcgcca gcgcctgcag caaggcgtcc atgtgcgtcc
     2641 ggctggccgc caggcgctcc cgctgcgggg tcgcgacctc gcccgacggt gcgcggacca
     2701 cctccagcag atccagctgg acctgccgca ggtccgcgtc cagatccgat gcgatgagca
     2761 cgccgggcac cgcctcctcc gtcagggtgt gaatcagcgc caggttgcgc tggagcgccc
     2821 cgccgcctgc cgccgccatg gcgaccagca agacgatggc gatggaggtc aggacgagga
     2881 tgtggcgacg gatgctcatg gggatacctg ccgggcggac atgacggatg acgcagcgca
     2941 acacgactgc gccggggata tcgacggccc gacggcaagc tgaaatgaat tatttgtgaa
     3001 ggcgcacgcc agcgcctgtc gcgtcctctc cgggccgctc gaagcgcccc ctgaacaccc
     3061 atttttgcct cgctttccat aagacatata tctattggca tattgaaaat caattttcca
     3121 tggacatata ctgtaacaat gttcacctat tgcctctgat gccatgaccg cgacagcgcc
     3181 cagcgcacgc cccttccggt gcccgtttcc gcgcctgctg cgcctggcct gcgccctcct
     3241 gctcatggtc agcacctccg acctcctcgc ccaggaagat gccgccgccc ttgaattcca
     3301 ccggcagcag gagcgcgaac ggcagctccg ccagcagcag gaaccccgcc cggatgtgcg
     3361 cctgcaggac gtctcaccaa gctcggcgcg cctcccccgc gacgaacacc cgtgctttcc
     3421 gatccgccgg atcgtcctga cgggcgtcga ggccgaacgc tttcaatggc tggcggatca
     3481 caccgggcgc gcagaggacg gccatcccga tcccgccatc ggcgcctgcc tcgggaccgg
     3541 cggtgtcaat ctcgtcatgc ggcgcctgca gaacgccctc atcgagcgcg gctacatcac
     3601 cacccgcatc ctcgccgcgc cccaggatct gtccaccggc accctgcacc tcaccgtggt
     3661 ccccggcaga atccacgccg tccgcttcct gccggacgcc tcaggccaga tgccctcccg
     3721 cgccacccac tggaacgcgc ttcccgccgc agcagacgat ctgctcaacc tgcgcgacat
     3781 cgaacaggcc ctcgagaact tcaagcgcct gcccagcgtc gaggccgacc tgcagatcgc
     3841 gccgtccgag gacgccgccg cgacaccggg cgacagcgac ctgctgatca cctggcagca
     3901 gaccttcccc ttccggctga cactctccgc cgacgattcg ggcacccagg ccagtggacg
     3961 ccatcaaggc gcagccaccg tcgccttcga caattggtgg acgctgaacg acctcttcta
     4021 catcacccat tcccagagcc tcttcacgga tggcacggtc cgtggcaccc gcggctatac
     4081 cgtccactat tcgctgcccc tcggctacag cctgctggcc ctgaccacca gccacaacac
     4141 ctatcaccag accgttgcgg gcctcaacgg cgctgtccgt tacgacggcg tcaacgacac
     4201 cggcgacctc cgtctcagcc acatcctgca ccgcaccgcc gcaggcaaga cgcatgtttt
     4261 cctgcgcggc tggcagcgcg cctccagcaa cttcacggat gacgaagagg tgctcgtaca
     4321 acgccgtcgc atggggggct gggaagccgg cgccggccac cgggccttca tcggtcaggc
     4381 cacactcgaa ggcacggtgg cctaccggcg cggcacggga gccttcgacg ccctgcccgc
     4441 cccggaagaa gcgcgcggcg ccatggcaga gggcaccgcg cgcccccgcc tgtggaccgc
     4501 cgacctgcag ttcaaccagc ccttccgcgt gggtggcgaa gccttccgct acggcacgtc
     4561 cctcagggcg cagtggaacg gaacccccct cgtaccccag gaccgcttcg ccatcggcgg
     4621 gcgctacacg gtgcgaggat tcgacggcga actcgtgctt gccgccgaac gcggctggtt
     4681 cgtccgcaac gacctgggct ggcagatcgg cggccaggaa ctgtatctgg gtctcgacca
     4741 cggggcagtc gccggccgca gcgccgccca gctgctcgga cgcaccctca cgggcgcagt
     4801 gatcggcctg cgcgggggcg ggagcgccct gtcctacgac ctcttcaccg gcacgccggt
     4861 agcccgaccc gagggcttcc aggccgcgcg gaccgtcgcc gggttctcca ttacctggac
     4921 gctctgacgc ccccccactt cgccccatac gcgcccccgc ccggagccca ccatgaaccg
     4981 actgtgttac cgcatcgtct tcaaccgtct gcgcggcatg ctgatggccg tcgccgaatg
     5041 cgcctgcgcg cgcagccgtt cgtccgggca aggccggcgc ccggtgccgc agcagccccc
     5101 accgccggca tccgtggcgg cgcgcctgcg acccaccgtc ctgcgctgct ggctggcctt
     5161 cgggctcgtg ctgcctgcgc tccccgcgcc cgctcagatc gtcgcctaca agaatccccc
     5221 tgccggtcag acacccacgg tgctggccgc ccccaacggc gtgccgctgg tcaatatccg
     5281 cacgcccaat gccgccggtc tgtcccacaa cgcctacagc cgtttcgacg tcccgcccca
     5341 gggcacgctc ctcaacaaca gccccgatgc cgtccagacc cagcttggcg gctgggtgca
     5401 aggcaacccc tggctggcaa ccggcagcgc ccggatcatc ctcaacgaag tcttcagcaa
     5461 tgaccccagc ctgctgcggg gctacatcga agtggccggt cagcaggccc aggtcgtggt
     5521 cgccaatccc tcgggcgtca cctgcacggg atgcggattc atccatgcac accgcgtgac
     5581 cctcacgaca ggcaccccgc gcgtcgccgc ggacggacgt ctggacgcgt accgcgtgac
     5641 gggtggcacg gtcctcatcg atggcaacgg actggacaca tcgagctccg atttcaccga
     5701 gatcattgcc cgcgcagtac gggtcaacgc cgggctgtgg gcgcggcagc tccatgtggc
     5761 cgccgggacc agcctcgtcg atgcggccac tctagacaca acgcccctcg ccagccccac
     5821 gccagccccc gccctggcca tcgatgtcgc cagcctgggc gggatgtacg cccgcaagat
     5881 caccctcgtg ggcaccgaag ccggcgtcgg cgtacgcagc gccggcacgc tgggcgcaag
     5941 catcggcgag ttcgtcatca gcgccgccgg acacctcgag aacaccggca cgctcacatc
     6001 cgccgatgca ctccgggtca cgacaacagg actccgtaac agcggcacgt tctatgcgca
     6061 gggcgaggcc cgcgtctcga cgaccggcgt cctcgagaac gatggcctca ttgcctc
//
LOCUS       DAOQMM010000143         5846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_7925_length_5846_cov_51.048869, whole genome shotgun sequence.
ACCESSION   DAOQMM010000143 DAOQMM010000000
VERSION     DAOQMM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7925_length_5846_cov_51.048869"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLH85_16030"
     CDS             <1..248
                     /locus_tag="PLH85_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66126.1"
                     /translation="PTRFPRLILRQKNPPTRFKNPNPGFSCLKMRQENTSTPKRNPEI
                     PFKNPRAGFLNTPDPFKNPPIPSKNGSGAFPWGGAEG"
     gene            complement(288..875)
                     /locus_tag="PLH85_16035"
     CDS             complement(288..875)
                     /locus_tag="PLH85_16035"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HNB66127.1"
                     /translation="MNGPDLLSLRQRLHTRLLELPVTARVAGDPNRSLFASLLAGQSA
                     GFGCLPAGLGLLPAQHTTLLEEFFPDALDASVLLVPLQRPVEAIPEWDDLYKLLTDHR
                     AGRHDAELWAARVIATACAGRDHLWQDLGFNNRTELSALITRNFPDLAAANSGDMKWK
                     KFLYRQFCAIEGIYVCPAPSCGVCADHAKCFGPEE"
     gene            complement(886..1248)
                     /locus_tag="PLH85_16040"
     CDS             complement(886..1248)
                     /locus_tag="PLH85_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB66128.1"
                     /translation="MALITFSSPMHKDKTVYAVAGSHTQTILSLAKEHHIPIDFGCQE
                     GNCGTCLVKVSSVDGKRRPMGGPLNVREVAALMDMGHITKAQVDQMYVDDIPPTQWRL
                     ACQMIVRDEDILVEYPSK"
     gene            complement(1378..1665)
                     /locus_tag="PLH85_16045"
     CDS             complement(1378..1665)
                     /locus_tag="PLH85_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB66129.1"
                     /translation="MPKAKVTFEDIGVSVTVPAGTRLIEVSEKVGAGITYGCREGECC
                     TCLTKIVSGADNLAPPSILEDQVLKDNMAPRQHRLACQAQIIGGEIVVRPA"
     gene            complement(1691..2962)
                     /locus_tag="PLH85_16050"
     CDS             complement(1691..2962)
                     /locus_tag="PLH85_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNB66130.1"
                     /translation="MAPVGLTESAVELAPGVHWVGAFDPHLRSFDIILKTANGTSYNA
                     YVVRGEKGVAVIDTVKEGFADTFFQRLESVARYDEITTIVLNHLEPDHSGALPELLRR
                     APQARVYLSVRAQMMLKALLKPTGEKAPDYTPVTTDDTVDLGGRTLRFLHTPYLHWPD
                     TQCTYLVEEAMLFSGDVFGCHFCDPRLFNDCVGDFRFSFEYYYAHIMRPFRENVLDAV
                     ALIEPLDLKLIAPAHGPVLRHAPRDYVHRYRELATPRLFNEARSEKTLVIFYLSAYGN
                     TRRMAECIAQGAERIRGVRVSLYDLEGGESDIFTDLVEEADGLAFGSPTINADAVKPI
                     WDLLSSLTTVNVKGKLGAAFGSYGWSGEAVRLIEDRLRGLKLRVPVEGLRIKLVPDEA
                     ELAQCCTLGESLARHLTGQLEHRELDMASLA"
     gene            complement(2940..3416)
                     /locus_tag="PLH85_16055"
     CDS             complement(2940..3416)
                     /locus_tag="PLH85_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNB66131.1"
                     /translation="MALVTFFEKPGCQGNARQKALLTAAGHIVEAHSLKAEPWTRERL
                     LQFLGGLPVADWFNRSAPAVKAGEIVPETLDADAAMALLLANPLLIRRPLMEVDGSRM
                     VGFDTAVVDAWIGLGEAERPAGNIEACVHGPAGHGRCGSAAADAEEEGHHGPCGTH"
     gene            complement(3416..3694)
                     /locus_tag="PLH85_16060"
     CDS             complement(3416..3694)
                     /locus_tag="PLH85_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB66132.1"
                     /translation="MALQITNNCVNCWACVDVCPNDAIYLDSPHFLIDDMKCTECLGD
                     HADPQCATICPIECAIVNELDEALNPLGSLTGIPMEKFIEMGVIKEAA"
     gene            complement(3755..5251)
                     /gene="nifB"
                     /locus_tag="PLH85_16065"
     CDS             complement(3755..5251)
                     /gene="nifB"
                     /locus_tag="PLH85_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HNB66133.1"
                     /translation="MDLPVISSNSDAEHSGGGCSSSGCGTAPDALSHLPDHIRAKVQD
                     HPCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCSNESRPGVVSELLSPDQAIKKV
                     LAVAAEIPQMTVLGIAGPGDPLANPDRTFETFRQLSDRAPDIKLCVSTNGLNLPQYVD
                     EIAKHNIDHVTITINCIDPEVGAQIYPWIYWNNKRIRGVEGARILIEQQQKGLEMLTS
                     RGILVKVNSVMIPGVNDHHLKEVSKVVKAKGAFLHNVMPLIAEAEHGTYYGIMEQRGP
                     TPEELQELQDACAGDMAMMRHCRQCRADAVGLLGEDRGAEFTLDKIDVMDVDYEAAMA
                     RRKEVHDAIIDQLEAKRGKKAPAADSDIPADARPVLMAVAGKNGVVAEHFGHAREFLI
                     YEASPAGVRFISHRKTELYCGGADTCGEGDEDPESLLARNIKVLEGCEVVLCSKIGYE
                     PWGMLEAAGIAPNGEHAMEPIEDAVMAVYKELAIAGRLSRPAPSKMVA"
     gene            5461..>5846
                     /locus_tag="PLH85_16070"
     CDS             5461..>5846
                     /locus_tag="PLH85_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB66134.1"
                     /translation="MKTYLDWSAYDTYGVGDAYAGIPATGGNYAKAVAVCMNNHQCQR
                     DGKGVMCPSYRITHDPAHSTGARVKAFKAALNGELGGAPGDNPFAHPDLAAAMDLCVS
                     CKGCKKECPSAVDMTLIKTEYLAQRNA"
BASE COUNT          948 a         1789 c         2061 g         1048 t
ORIGIN      
        1 cccccaccag atttccccgc ctcattttga ggcagaaaaa tcccccaacc agatttaaaa
       61 atccgaaccc cggattttct tgcctaaaaa tgaggcaaga aaatacctcc accccaaaaa
      121 gaaatccgga aatcccattt aaaaatcccc gcgccggatt tttaaatacc ccggacccat
      181 ttaaaaatcc gcccatccca tcaaaaaatg gttccggggc atttccatgg ggcggggccg
      241 agggatgacg tcgcagctca gcgaggatag aggacggagc gttccgctca ctcctccggt
      301 ccgaagcact tggcgtggtc ggcgcagacg ccgcaggacg gcgccgggca gacgtagatg
      361 ccttcgatgg cgcagaactg acggtagagg aacttcttcc acttcatgtc gccgctgttg
      421 gcggcggcca ggtcggggaa gttgcgggtg atcagggcgg acagctcggt gcggttattg
      481 aagcccaggt cctgccacag gtggtcgcgg ccggcgcagg cggtggcgat gacgcgggcg
      541 gcccacagct cggcgtcgtg gcggccggcg cggtggtcgg tgaggagctt gtagaggtcg
      601 tcccactcgg ggatggcctc caccgggcgc tgcaggggca cgaggaggac gctggcgtcg
      661 agggcgtccg ggaagaattc ttccagcagt gtggtgtgct gggcggggag caggcccagc
      721 ccggcgggca gacagccgaa accggcactc tgcccggcga gcaggctggc aaacaatgaa
      781 cggttcggat cgccggccac cctggccgtc accggcagtt ccagcagccg cgtgtgcagc
      841 cgctggcgga ggctgaggag atccggaccg ttcatggcag gggtctcact tggaggggta
      901 ttccaccagg atgtcttcgt cgcggacgat catctggcag gcgagacgcc actgggtggg
      961 ggggatgtca tccacgtaca tctggtccac ctgggccttg gtgatgtggc ccatgtccat
     1021 cagggcggcc acttcgcgga cgttcagggg gccgcccatg ggacggcgct tgccatccac
     1081 ggaggagacc ttgacgagac aggtgccgca gttgccttcc tggcagccga agtcgatggg
     1141 gatgtggtgt tccttggcaa gggaaaggat ggtctgggtg tggctgccgg caacggcata
     1201 cacggtcttg tccttgtgca tcggactgct gaaggtgata agggccatgg tgggttctcc
     1261 tgacgaacgg ttgataggcg atattctctt tgggggggcg cctgccccgt agggcgggga
     1321 aggccggacc gcgtccggca gttcccgctg ggcttgttgt gtcgggtgtg gccgcgatca
     1381 ggcggggcgg acgacgatct cgccgccgat gatctgggcc tggcaggcca gccggtgctg
     1441 acgtggggcc atgttgtcct tgagcacctg gtcttccagg atgctgggcg gggcgaggtt
     1501 gtcggcgccg gagacgatct tggtcaggca ggtgcagcat tcgccttcgc ggcagccgta
     1561 ggtgatgccg gcgccgactt tttccgagac ttcgatgagc cgggtaccgg ccgggacggt
     1621 gacggagaca ccgatgtctt cgaaggtgac tttggctttg ggcatttttc gcttccttga
     1681 gagggcaata tcaggcgagg ctcgccatat ccagttcccg gtgttccagc tggccggtga
     1741 gatgacgggc caggctttcg ccgagggtgc agcattgcgc gagttccgct tcgtcgggca
     1801 ccagcttgat gcgcaggccc tccaccggca cccgcagctt gaggccgcgc aggcggtcct
     1861 cgatcaggcg caccgcctcg ccgctccagc cgtaggagcc gaaggccgcg ccgagcttgc
     1921 ccttgacgtt gacggtggtg agggaggaga gcaggtccca gatgggcttg accgcgtccg
     1981 cgttgatggt ggggctgccg aaggcaaggc cgtcggcttc ctccaccaga tcggtgaaga
     2041 tgtccgactc gccgccttcc aggtcgtaga gggagacgcg cacgccgcgg attcgctcgg
     2101 cgccctgggc gatgcactcg gccatgcgcc gggtgttgcc gtaggccgag aggtagaaga
     2161 tcaccagggt cttttcgctg cgggcctcgt tgaagaggcg cggagtggcg agctcccggt
     2221 agcggtgcac gtagtcccgc ggcgcatggc gcagcaccgg gccgtgggcg ggggcgataa
     2281 gcttcaggtc gaggggttcg atcagcgcca ctgcgtcaag gacgttctca cggaacggcc
     2341 gcatgatgtg ggcgtagtag tactcgaagg agaagcggaa gtcgcccacg cagtcgttga
     2401 acaggcgcgg gtcgcagaag tggcagccga acacgtcgcc ggagaacagc atcgcctctt
     2461 cgaccaggta ggtgcactgg gtgtcgggcc agtgcaggta gggcgtgtgc agaaagcgca
     2521 gggtgcggcc gcccaggtcc accgtgtcgt cggtggtgac cggggtgtag tccggggcct
     2581 tctcgccggt gggcttgagc agggccttga gcatcatctg ggcgcgtacc gacaggtaca
     2641 cccgcgcctg gggtgcgcgc cgcagcagct cgggcagggc gccgctgtgg tccggttcca
     2701 ggtggttgag gacgatggtg gtgatctcgt cgtaacgggc gacggattcc aggcgctgga
     2761 agaaggtgtc agcgaagcct tccttcaccg tgtcgatgac cgccaccccc ttctcgccgc
     2821 gcaccacgta ggcgttgtag ctggtgccgt tggcggtctt gaggatgatg tcgaagctcc
     2881 gcagatgcgg gtcgaaggcg cccacccagt ggacgcccgg agccagttcg acggcggatt
     2941 cagtgagtcc cacaggggcc atggtgccct tcttcctctg cgtccgcggc agcgctgcca
     3001 cagcggccgt ggcccgccgg gccatgcacg caggcttcga tgttgccggc ggggcgctcc
     3061 gcctcgccca gaccgatcca ggcgtctacc acggcggtgt cgaagccgac catccggctg
     3121 ccgtccacct ccatcagcgg gcggcggatc agcagggggt tggccaggag cagggccatc
     3181 gcggcgtccg cgtccagcgt ttccgggacg atctccccgg ccttcacggc cggggcggaa
     3241 cgattgaacc agtcggccac gggcaggccg ccgaggaact ggagcaggcg ctcccgcgtc
     3301 cacggctcgg ccttgaggct gtgcgcctcc acgatatgcc cggcggccgt gagcagcgcc
     3361 ttctggcgcg cgttgccctg gcagccgggc ttctcgaaga aggtgacgag ggccatcagg
     3421 cggcttcctt gatcacgccc atctcgatga acttctccat cgggatgccg gtgagggagc
     3481 ccagcgggtt gagcgcctcg tccagctcat tgacgatggc gcactcgatc gggcagatcg
     3541 tcgcgcactg gggatcggcg tgatcgccca ggcactcggt gcacttcatg tcgtcgatca
     3601 ggaagtgagg cgaatccagg tagattgcgt cgttggggca cacgtccacg caggcccagc
     3661 aattgacgca gttgttggtg atttgcagtg ccatgttcga atctccgggt gggccgcccc
     3721 ccgcaggggg cggcagatca cgagggcagg gaggtcaggc caccatcttc gacggggcgg
     3781 ggcggctgag gcggccggcg atggccagtt ccttgtacac cgccatcacc gcgtcctcga
     3841 tgggctccat ggcgtgctcg ccgttggggg cgatgccggc ggcttccagc atgccccacg
     3901 gctcgtagcc gatcttggag cacagcacca cctcgcagcc ttcgagcacc ttgatgttgc
     3961 gggcgagcag ggactcggga tcctcatcac cttcaccgca ggtgtcggcg ccgccgcagt
     4021 acagctcggt cttgcggtgg ctgatgaagc gcacgccggc gggcgaggct tcgtagatga
     4081 ggaactcgcg ggcgtgcccg aagtgctcgg ccaccacgcc gttcttgccg gccacggcca
     4141 tcagcaccgg gcgggcgtcg gccgggatgt cgctgtcggc ggccggcgcc ttcttgccgc
     4201 gcttggcttc gagctggtcg atgatggcgt cgtggacttc cttgcggcgg gccatggcgg
     4261 cctcgtaatc cacatccatg acgtcgatct tgtccagggt gaactcggcg ccccggtctt
     4321 cgcccagcag gcccaccgcg tcggcgcggc actggcggca gtggcgcatc atcgccatgt
     4381 cgccggcgca ggcgtcctgc agctcctgga gctcttcggg cgtggggccg cgctgctcca
     4441 tgatgccgta gtaggtgccg tgctccgcct cggcgatgag gggcatcacg ttgtgcagga
     4501 aggcgccctt ggccttgacg accttggaga cttccttgag gtgatggtca ttgacgccgg
     4561 gaatcatcac cgagttgacc ttcaccagga tgccccgcga ggtgagcatc tcgaggccct
     4621 tctgctgctg ctcgatgagg atcctggcgc cctcgacgcc gcggatgcgc ttgttgttcc
     4681 agtagatcca cggatagatc tgggcgccga cctcggggtc gatgcagttg atcgtgatgg
     4741 tcacgtggtc gatgttgtgc ttggcgatct cgtccacgta ctgcggcagg ttgaggccgt
     4801 tggtggacac gcagagcttg atgtccggtg cccggtccga gagctgacgg aaggtctcga
     4861 aggtgcggtc cgggttggca agggggtctc ccggtccggc gatgcccagc acggtcatct
     4921 gggggatctc cgcagccacg gcgaggacct tcttgatggc ctggtccggc gacagcagct
     4981 ccgacaccac gccggggcgg gattcgttgg agcagtcgta cttgcggttg cagtagttgc
     5041 actggatgtt gcaggccggg gcgacggcca cgtgcattcg agcgaaatag tggtgcgctt
     5101 cttcggagta acaggggtga tcctgcacct tggcgcgtat gtgatcgggc agatgagaga
     5161 gggcatccgg cgcagtgccg cagccgctcg acgaacagcc gccgccgctg tgctcggcgt
     5221 cgctgttgct gctgatgacg ggaaggtcca cgataaggct cctctgggtt ggtgcagagg
     5281 actcagcaac gcttgtgcca gggcagaaac tgcttgaaaa acatcgcgct agcgggaagg
     5341 cacggcgcag cttgtcggcc ctttgtcgca aacgcgacag cctgccacct tggcaagccg
     5401 ctgattcgtg gcggttttgt cgcatcgcgg ggctggcacc cgtcttgcag catttcgccc
     5461 atgaaaacct atctcgactg gtcggcctac gacacctacg gcgtcggcga cgcctacgcg
     5521 ggcattcccg ccaccggcgg caactacgcc aaggcggtgg cggtgtgcat gaacaaccac
     5581 caatgccagc gcgacggcaa gggcgtgatg tgccccagct accgcatcac ccacgacccg
     5641 gcccactcca ccggcgcccg cgtcaaggcc ttcaaggccg ccctcaacgg cgagctgggc
     5701 ggggcgccgg gcgacaatcc cttcgcccac ccggacctgg ccgccgcgat ggacctgtgc
     5761 gtgtcctgca agggctgcaa gaaggaatgc cccagcgcgg tggacatgac cctcatcaag
     5821 accgaatacc tggcccagcg caacgc
//
LOCUS       DAOQMM010000144         5787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_8053_length_5787_cov_39.136776, whole genome shotgun sequence.
ACCESSION   DAOQMM010000144 DAOQMM010000000
VERSION     DAOQMM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_5787_cov_39.136776"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLH85_16075"
     CDS             <1..663
                     /locus_tag="PLH85_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66135.1"
                     /translation="PASAAPHPPKPAPTQTARAERRPPVLTAQAPSSARALAASPTWS
                     IAQKQEMDRFLDDLATDARRAPSLAERSLAMAREIGREQARRNDDAYDLVERRPNSPE
                     IDPLSLEMYMESLIRKLNKAAGFVRNDPRTRGVQTASVLVRIAPDGSLESFKVLRVAD
                     QQSEIEFTHAVVRQASPFAAFPPDIQRSARSLAMLICIRPPSLSGGAFGFTRMTEGRG
                     CG"
     gene            695..1249
                     /locus_tag="PLH85_16080"
     CDS             695..1249
                     /locus_tag="PLH85_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66136.1"
                     /translation="MPPMSRRPPRGGRLVLPALTALLAACAGFGPAPDAATGPRTRDA
                     LVMHLGPPALRWPESDGGERLAFPTGPMGVHTWMARVDTTGRVLTVENVLVPARFAEI
                     QPGMGQDEVLRTLGPPQPHWTIYYKARDELVWEWRFCDDFQEPARFDVLFDGTSGRVR
                     STLAQPERLSNPWGRGGRRDWCSR"
     gene            1284..2780
                     /gene="glpK"
                     /locus_tag="PLH85_16085"
     CDS             1284..2780
                     /gene="glpK"
                     /locus_tag="PLH85_16085"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNB66137.1"
                     /translation="MTFLLALDQGTTSSRAIVFDAAGQAVASAQQEFRQHYPQPGWVE
                     HDADEIWTTQRDCVRLALARAGLAAADIAALGIANQRETTVLWERASGRPLAPAIVWQ
                     DRRTAGHCDRLRAAGHADLIRARSGLELDAYFSATKLAWLLDHVPDARRRAEAGELAF
                     GTLDSWLVWQLTGGTRHVTDPSNAARTMLFNLHSLAWDDTLLDLFAIPRTLLPEVVDT
                     AGVLGATRPDLFGAPIPIAALAGDQQAATFGQACFAPGSAKNTYGTGCFLMLNTGATP
                     VASTQRLLSTVGWRLPGETCYMLEGSVFIGGAVIQWLRDGLGLIEHAADVEALAASVP
                     DSGGALLVPAFAGLGAPWWDGHARGTLVGLTRGVTRAHIARAALDAIALQTVDLVDAM
                     NRDGAGPLRELRVDGGAARNDLLMQIQADLLGVPVIRPRQTETTALGAACLAGLAAGV
                     WRSPAELARHWQEERRFEPTRSADWRAARLHDWHRAVARSRAWAEDAP"
     gene            2777..4297
                     /locus_tag="PLH85_16090"
     CDS             2777..4297
                     /locus_tag="PLH85_16090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNB66138.1"
                     /translation="MKRADRLIRLRHTARWDILIVGGGATGLGCAVDAAARGHSTLLL
                     EAGDFAGGTSSRSTKLIHGGVRYLAQGRLGLVREALAERTRLLRNAPHLVHPQGFVVP
                     VHGAWEGLRLGLGLRLYDHLAGADGLGPCRSLSADATLAALPGLRPDGLRGGLRYQDA
                     RFDDARLAITLMRSAEALGALTLNALRVTGLLHAGGRVAGVAAEDGETGEHFELPARV
                     VINATGVWSDAVRRLDDPDAPPQLAPSQGAHVVVDRDFLPGDDALLIPRTDDGRVLFV
                     IPWQGRLLLGTTDTPRTDLPAEPRPLDAEIDFILTTAARYLTRAPRRDDVRSAFAGLR
                     PLIGGTGASARLSREHLIQVSTRGLVSVAGGKWTTYRSMAEAVVDRAEAVGGLPPRPC
                     PTPTLRLFGAPTAPTGDVYGCERAAIERLPGAGRRLHPAFTLSEAEVRHAARAEQARS
                     VADVLARRHRALFIDAAAAQQAAPAVAAVMASELGWDAPRTHEMTEAFHRLAEGFR"
     gene            complement(4331..>5787)
                     /locus_tag="PLH85_16095"
     CDS             complement(4331..>5787)
                     /locus_tag="PLH85_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNB66139.1"
                     /translation="NNANLSTGGTATDVTDDVHPEVAERAIAAAKNIGLDICGVDVVC
                     DSVLEPLEAQSGGIVECNAAPGLRMHLQPSYGKGRPVGEAIVGNMFAPGENGRIPVVA
                     VAGTNGKTTTVRLTAHLLGVTGKRVGMTNSDGVYVGARRIDTGDCSGPRSARNILMHP
                     DVDAAVFETARGGVLREGLAFDRCDVAVVTNIGMGDHLGLSYISTVEDLAVVKRVIVQ
                     NVAPHGTAVLNAADPMVVRMASACPGSVLFFAADEHNPVLATHRVQGHRVVFVDEDAI
                     VAAQKDREFRIPLAQIPLTRNGTISFQVENAMASIAAAWALGIDPETIRAGVATFVND
                     AATAPGRFNVFDYKGATVIADYGHNPDAILALVQAVESIPAKRRSVVISGAGDRRDED
                     LRRQTQILGDIFDEVVLYQDLCQRGRADGEVLALLREGLTQAKRATEVLEIRGEFLAI
                     DTAFAKLQPGDLCLVLVDQVEEALEHIARRIAEA"
BASE COUNT          773 a         2334 c         1894 g          786 t
ORIGIN      
        1 cccgcctccg ctgccccaca cccgcccaaa cccgcgccca cccagaccgc ccgcgccgag
       61 cgccgcccac ccgtcctcac cgcccaggcc ccgtcgtccg cccgcgcgct ggcggcatcc
      121 cccacctgga gcatcgccca gaagcaggag atggaccgct tcctggacga cctggcaacc
      181 gatgcccgcc gcgcccccag cctcgccgag cgctccctgg ccatggcgcg ggagatcggc
      241 cgcgagcagg cccgccgcaa cgacgatgcc tacgatctcg tcgagcgccg gcccaacagc
      301 cccgaaatcg accccctgag cctcgagatg tacatggagt ccctcatccg caagctcaac
      361 aaggccgccg gttttgtccg gaacgacccg cgcacccgcg gcgtgcagac ggcgtcggtg
      421 ctggtgcgca tcgcgccgga cggcagcctg gaaagcttca aggtgctgcg cgtggccgac
      481 cagcagtccg agatcgaatt cacccacgcc gtcgtccgcc aggcgtcgcc cttcgccgcc
      541 tttccgccgg acatccagcg ctccgcgcgc tccctggcca tgctgatctg cattcgtccg
      601 ccgtccctca gcggcggcgc cttcggcttc acgcggatga ccgaggggcg cggctgcggc
      661 tagaatgccg acatcgtcgc gcggagccgt caccatgcct ccgatgtcgc gccgcccgcc
      721 ccgcggcggc cgcctcgtcc tgcccgccct cacggccctg ctcgccgcct gcgccggttt
      781 cggacccgcg cccgacgccg ccaccggccc gcgcacccgc gatgccctcg tgatgcacct
      841 cggcccgccg gctttacgct ggcccgagtc cgacggcggc gagcgcctcg cctttcccac
      901 cggcccgatg ggcgtccaca cctggatggc ccgcgtcgac accaccggcc gcgtcctcac
      961 cgtcgagaac gtcctggtcc cggcccgctt cgccgagatc cagcccggca tgggccagga
     1021 tgaagtcctg cgcaccctcg gcccgcccca gccccactgg accatctact acaaggcccg
     1081 cgacgagctc gtgtgggaat ggcgcttctg cgacgacttc caggagcccg cccgcttcga
     1141 cgtgctcttc gatggcacca gcggccgggt acgcagcacc ctggcccagc ccgagcgcct
     1201 ctccaatccc tggggacgcg gtggccggcg cgactggtgc agccgctgac cgccctcctc
     1261 ccgaccgccc gcggagactg cccatgacct tcctcctcgc cctcgaccag ggcaccacca
     1321 gctcccgggc catcgtcttc gacgccgccg gccaggccgt cgccagcgcc cagcaggaat
     1381 tccgccagca ctacccgcag cccggctggg tcgagcacga cgccgacgag atctggacca
     1441 cccagcgcga ctgcgtccgc ctggccctcg cccgggccgg tctcgccgcg gccgacatcg
     1501 cagccctggg catcgccaac cagcgggaaa ccaccgtgct gtgggagcgg gccagcggac
     1561 ggcccctcgc ccccgccatc gtgtggcagg accgccgcac cgccggccac tgcgatcgcc
     1621 tgcgtgccgc cggtcacgca gacctgatcc gcgcccgcag cggtctggag ctggacgcct
     1681 acttctctgc caccaagctc gcctggctgc tggaccacgt ccccgacgcc cggcgccgcg
     1741 ccgaagccgg cgagctggcc ttcggcaccc tcgacagctg gctggtgtgg cagctcaccg
     1801 gcggcacacg acacgtcacc gacccgagca acgcagcccg cacgatgctc ttcaacctgc
     1861 acagcctggc ttgggacgac acactcctcg acctcttcgc gatcccccgc accctgctgc
     1921 cggaggtggt ggacacggcc ggcgtcctgg gcgccacgcg gccggatctg ttcggcgccc
     1981 ccatccccat cgccgccctg gccggcgacc agcaggccgc caccttcggg caggcctgct
     2041 tcgcgccggg cagcgccaag aacacctatg gcaccggctg cttcctgatg ctcaacaccg
     2101 gcgccacgcc ggtcgcctcc acccagcgcc tgctcagcac cgtcggctgg cgcctgcccg
     2161 gcgagacctg ctacatgctg gaaggcagcg tcttcatcgg cggcgcggtg atccagtggc
     2221 tgcgcgacgg cctcggcctg atcgagcacg ccgcggatgt ggaagccctc gccgccagcg
     2281 tgcccgacag cggcggcgcc ctcctcgtcc cggcctttgc gggcctcggc gccccctggt
     2341 gggacggcca cgcccgcggc accctggtcg gcctgacccg cggtgtcacc cgtgcccaca
     2401 tcgcccgcgc cgcgctggac gccatcgccc tacagaccgt cgacctggtg gacgccatga
     2461 accgggacgg cgccggcccc ctccgcgaac tgcgcgtgga tggcggcgct gcccgcaacg
     2521 acctgctcat gcagatccag gccgacctgc tgggcgtccc cgtgatccgc ccgcgccaga
     2581 ccgagaccac cgccctcggc gccgcctgcc tggccggact ggcggcgggc gtgtggcgtt
     2641 cgccggcgga actcgcccgg cactggcagg aggaacgccg cttcgagccc acccgctccg
     2701 ccgactggcg cgccgcgcgc ctccacgact ggcaccgggc cgtggcgcgc agtcgcgcct
     2761 gggccgaaga cgccccgtga agcgcgccga ccgcctgatc cgcctgcgcc acacggcgcg
     2821 ctgggacatc ctcatcgtcg gcggcggcgc caccggcctg ggctgcgccg tggatgcggc
     2881 cgcccgcggc cactccaccc tgctgctgga agccggcgac tttgccggcg gcaccagttc
     2941 ccgctccacc aagctgatcc atggtggcgt gcgctacctg gcccagggcc gcctcgggct
     3001 ggtccgcgaa gccctcgccg aacgcacccg cctgctccgc aacgcgcccc acctggtcca
     3061 tccccagggc ttcgtggtgc cggtccacgg cgcctgggag ggtctgcgcc tgggcctcgg
     3121 cttgcgcctg tacgaccacc tcgccggcgc agacggtctg ggcccctgcc gcagcctgtc
     3181 cgcggacgcc accctcgccg ccctgcccgg cctgcgtccg gatggcctgc gcggcggcct
     3241 ccgctaccag gacgcccgct tcgacgacgc gcgcctggcc atcaccctga tgcgcagcgc
     3301 cgaagccctg ggcgccctca ccctcaacgc cctgcgcgtc accggcctgc tccacgcggg
     3361 cggtcgcgtc gccggcgtgg cagccgagga tggcgagacc ggcgaacact tcgagctgcc
     3421 cgcgcgggtc gtcatcaacg ccaccggggt gtggagcgat gccgtgcgac gcctcgacga
     3481 cccggacgca ccgccgcagc tggcccccag ccagggcgcc cacgtggtgg tggaccggga
     3541 tttcctgccc ggcgacgacg ccctcctgat cccccgcacc gacgacggcc gggtgctttt
     3601 cgtcatcccc tggcagggcc gtctgctcct cggcaccacc gacacaccgc ggaccgacct
     3661 gccggccgag ccccgcccgc tggacgccga gatcgacttc atcctcacca ccgccgcccg
     3721 ctacctgacc cgtgcgccga ggcgggacga cgtgcgcagc gcctttgccg gcctgcgccc
     3781 cctgatcggc ggcaccggcg ccagcgcccg cctgagccgc gagcacctca tccaggtctc
     3841 gacccgcggc ctcgtcagcg tggcgggcgg aaaatggacc acttaccgga gcatggccga
     3901 ggcggtggtg gaccgcgccg aggcggttgg cggcctgccc ccacgaccct gcccgacccc
     3961 cacgctacgc ctcttcggcg ccccaaccgc gccgaccggc gatgtgtacg gctgcgagcg
     4021 cgcagccatc gagcgtctgc ccggcgccgg ccggcgtctg catcctgcct tcaccctgag
     4081 cgaggccgag gtgcgccatg cagcgcgcgc agaacaggcc cgcagcgtgg ccgacgtcct
     4141 cgcccgccgc caccgggccc tgttcatcga cgccgccgcc gcccagcagg ccgcccccgc
     4201 cgtggcggcg gtcatggcca gcgaactggg ctgggatgca ccgcgcaccc atgaaatgac
     4261 ggaagccttc caccgtctgg cggaaggctt ccggtaagcc cacgccggcc ccagggccgg
     4321 gcctcgcggg tcaggcttcg gcgatgcgcc gggcgatgtg ctccagcgcc tcttccacct
     4381 gatccaccag caccaggcac aggtcgcccg gctggagctt ggcaaaggcg gtgtcgatgg
     4441 cgaggaactc gccgcggatc tcaaggacct cggtggcccg cttggcctgg gtcagccctt
     4501 cccgcagcag agccagcacc tcgccgtcgg cgcggccacg ctggcacaga tcctggtaca
     4561 gcaccacctc atcaaagatg tcgccaagga tctgggtctg gcggcgcagg tcctcgtcgc
     4621 ggcggtcgcc ggcgccgctg atcaccaccg agcgacgctt ggccgggatg ctctcgacgg
     4681 cctgcaccag ggccaggatg gcgtccgggt tgtggccgta gtcggcgatc acggtggcgc
     4741 ccttgtaatc gaacacgttg aagcgtccgg gcgccgtggc ggcatcattc acgaaagtcg
     4801 ccacgccggc gcggatcgtc tccggatcga tgcccagggc ccacgccgcg gcgatggagg
     4861 ccatggcgtt ctccacctgg aaagagatgg tgccgttgcg ggtgagggga atctgcgcca
     4921 gcggaatgcg gaattcccgg tccttctggg cggcgacgat ggcgtcctca tcgacaaaga
     4981 ccacccggtg accctgcacg cggtgggtgg cgagcaccgg gttgtgctcg tcggcggcaa
     5041 agaacagcac cgagccgggg caggcgctgg ccatcctgac gaccatcggg tcggccgcgt
     5101 tgagcaccgc ggtgccgtgg ggcgccacgt tctgcacgat gacccgcttg accaccgcca
     5161 gatcctcgac ggtgctgatg taggacaggc ccaggtggtc gcccatgccg atgttggtga
     5221 ccactgccac gtcgcagcgg tcgaaggcga ggccttcccg cagcacgccg ccgcgggcgg
     5281 tctcgaacac cgccgcgtcc acgtccggat gcatgaggat gttgcgggcg ctgcgcgggc
     5341 ccgagcagtc gcccgtgtca atgcgccgcg cgcccacgta gacgccgtcc gagttggtca
     5401 tgcccacgcg cttgccggtg acgcccagca aatgcgcggt gagacgcact gtggtggtct
     5461 tgccgttggt gcccgccacc gccaccaccg ggatgcggcc gttctcgccg ggcgcgaaca
     5521 tgttgccgac gatggcctcg cccaccgggc ggcccttgcc gtaggacggc tgcaggtgca
     5581 tgcgcaggcc gggcgcggca ttgcattcga cgatgccgcc gctctgggct tcgaggggct
     5641 ccagcacgct gtcgcacacc acgtccacgc cgcagatgtc gaggccgatg ttcttggccg
     5701 cggcgatggc ccgctcggcc acctcggggt ggacgtcgtc ggtcacgtcg gtggcggtgc
     5761 cgccggtgga caggttggcg ttgttgc
//
LOCUS       DAOQMM010000145         5698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_8254_length_5698_cov_39.979798, whole genome shotgun sequence.
ACCESSION   DAOQMM010000145 DAOQMM010000000
VERSION     DAOQMM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8254_length_5698_cov_39.979798"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1224)
                     /gene="purM"
                     /locus_tag="PLH85_16100"
     CDS             complement(190..1224)
                     /gene="purM"
                     /locus_tag="PLH85_16100"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNB66140.1"
                     /translation="MSSLTYRDAGVDIDAGDALVDRIKPFAKRTMRPEVLGGIGGFGA
                     LFEISRKFKEPVLVSGTDGVGTKLKLAFQLNKHDTVGQDLVAMSVNDILVQGAEPLFF
                     LDYFACGKLDVDTAATVVEGIARGCELAGCALIGGETAEMPSMYPDGEYDLAGFAVGA
                     VEKSQIIDGSTIRPGDVVLGLASSGAHSNGYSLIRKIIELTQPNLDGDFHGQTLKDVI
                     LAPTRIYVKSLLALMAQRPGLVKGMAHITGGGLLENVPRVLADHLTAVLRQDAWEMPY
                     LFQWLKGAGNVEAQEMYRVFNCGIGMVVIVAAEDADAAAAQLRSTGETVYTLGTIEAR
                     GEGQAQTVVV"
     gene            1458..2141
                     /gene="hda"
                     /locus_tag="PLH85_16105"
     CDS             1458..2141
                     /gene="hda"
                     /locus_tag="PLH85_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNB66141.1"
                     /translation="MKQLTLDLLADAPPLLDSFVPGANADLLAALFECVPVADTPQHL
                     YLWGDAGSGRSHLLRGVVTAAAQAGRPACYLRAADVVDTLPDAPALLLAVDDVERLGA
                     DAQIALFRACNAARGQRMTLVTSGAVPPRDLPLREDLRTRIGQSLIFEVKALGDDERA
                     AILAAHADARGMRLEADLVQYLLRHAPRDLKSLLRTVDALDAASLERKRAITLPLLRD
                     VLQHVPPTA"
     gene            2148..2810
                     /locus_tag="PLH85_16110"
     CDS             2148..2810
                     /locus_tag="PLH85_16110"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNB66142.1"
                     /translation="MELVLFDLDNTLLAGDSDFEWAQFLIAKGVLDKEVYEARNLAFY
                     EQYKAGTLDIYEFLDFQLLPLARHSREQLDLWHREFMDSRIRPMMTPAARALVRRHLD
                     SRAVVAIVTATNSFVTGPIGRAFEIPHLVATVPAQEHGVFTGKPRGTPAFKAGKIERV
                     ENWLEALGLHWGSFRRSWFYSDSHNDLPLLEKVSDPVAVDPDDTLRAHATARGWNILS
                     LR"
     gene            complement(2841..3722)
                     /locus_tag="PLH85_16115"
     CDS             complement(2841..3722)
                     /locus_tag="PLH85_16115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNB66143.1"
                     /translation="MHDTDTVTDPDFDSLVPGQSVGRRTFVGAALAAGFALSVRPALG
                     QDVIHTPADGLLAGEVRIVAPDGVALAAYRARPAGDGPVPVVLVVQEIFGVHEYIRDV
                     CRRLARAGYLAIAPNLFQRQGDPSALASVAEIQANIVAKVPDAQVLGDLEACAEWALT
                     QGGDGRLGITGFCWGGRIAWLFAARHAGLKAAVAWYGRLDGTPSANTPTHPLDVVEAL
                     QAPVLGLYGAKDQGIPVSDVEAMRERLAAAGKRGELVLYPDAGHAFHADYRPSYRAAE
                     AADGWARLLAWFRANGL"
     gene            complement(3954..4784)
                     /locus_tag="PLH85_16120"
     CDS             complement(3954..4784)
                     /locus_tag="PLH85_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HNB66144.1"
                     /translation="MESLPLDSPSLVALVFLLGLKHGFDADHLATIDGLTRFNARSRP
                     RLARLCGTLFSLGHGAVVLAVAVGASLLSRGWQAPEWLELSGAGISILFLLGLGVLNL
                     GAVLAAPRDAVVRPLGLKGRWLGRLAEVSHPAGVALVGALFALSFDTLSQAALFAVAG
                     GSGVGGGALRAAGLAGCFVLGMLVADGANGFWIARLIARADGVARIASRVMALAVAGM
                     SLLVAAYGLARLLSPRIEAWGEGKELLLGAVVVTVMAASFVVGLRLARIQGAPAAARG
                     "
     gene            complement(4948..>5698)
                     /locus_tag="PLH85_16125"
     CDS             complement(4948..>5698)
                     /locus_tag="PLH85_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB66145.1"
                     /translation="LGRLVAGVAHELNNPISFILGNTHALRRYAQRLEEYLAAVHGGV
                     GAEALADLRERLRIDRILKDLEPLIDGTIEGAERTRDIVDGLKRFSALDREENARFDL
                     AAVIDRSVTWVSKAAPERFRVTVHLPPGLQVTGSAGQMQQVVMNLVQNACDATAGQHP
                     ARLEIRGQIAAAGARREVVLDFHDNGSGIEDRHLPHIFDPFFTTKPVGSGTGLGLSIS
                     YGIVERHGGRLTAGNHPRGGACFTLRLPLAD"
BASE COUNT          880 a         2152 c         1921 g          745 t
ORIGIN      
        1 gcatgcgggg cgagtgacgc gcatcgccgg caccgttccc aggtcggcga cgtcgggggt
       61 acaggggccg ggcaacacgc accgcatccc tacccctctt cactgcagat caacgggcca
      121 caaaaacggg cccgcaggcc cgttagattc actaccgaaa gcggcgtccg ccgcgtcagc
      181 gctaccgcct tacaccacca ccgtctgcgc ctgaccttcc ccgcgggctt cgatggtgcc
      241 gagggtgtac accgtctcgc ccgtggaacg cagttgcgca gccgccgcgt cggcgtcttc
      301 ggcggcgacg atcaccacca tgccgatgcc gcagttgaag acacggtaca tctcctgagc
      361 ctccacgttg ccggcgccct tgagccactg gaagaggtag ggcatctccc aggcgtcctg
      421 gcggaggacg gcggtcaggt ggtcggcgag gacgcggggc acgttctcca ggaggccgcc
      481 gccggtgatg tgggccatgc ccttcaccag gccggggcgc tgggccatca gggccagcag
      541 ggatttcacg tagatgcggg tcggggcgag gatcacgtcc ttgagggtct ggccgtggaa
      601 gtcgccgtcc aggttgggct gggtgagctc gatgatcttg cggatcaggg agtagccgtt
      661 ggagtgggcg ccgctggagg caagacccag caccacgtcg ccgggacgaa tggtggagcc
      721 gtcgatgatc tgggacttct cgaccgcgcc gacggcgaag ccggccaggt cgtattcgcc
      781 gtccgggtac atgctgggca tctcggcggt ctcgccgccg atgagggcgc agccggccag
      841 ctcgcagccg cgggcgatgc cttccaccac ggtggcagcg gtgtccacgt ccagcttgcc
      901 gcaggcgaag tagtccagga agaacagggg ctcggcgccc tgcaccagga tgtcgttgac
      961 gctcatggcc accagatcct ggcccacggt gtcgtgcttg ttgagctgga aggccagctt
     1021 gagcttggtg cccacgccgt cggtgccgga caccagcacc ggctccttga acttcctgga
     1081 gatctcgaag agggcgccga agccgccgat gccgcccagc acctcggggc gcatggtgcg
     1141 cttggcaaag ggcttgatgc ggtccaccag ggcatcgccg gcgtcgatat ccacaccggc
     1201 atcgcgataa gtaagggagc tcatgggctg tgatcttctc tggaacggcc ggcggcaggg
     1261 ggttgaggca ctttcgggac gctattcggt tgagcgcccc gccgcgaccg gctacattaa
     1321 cgggccttgc gcgggttccg gccggcggga agacttcccg ggccccgatc cacgcgcgca
     1381 aacccggatt ttagctgaat tgtgacccga ccgcgtcgct caccaccgcc cgacgccccg
     1441 gccccccgcc gcgccccgtg aagcagctca ccctcgatct cctcgccgac gccccgcccc
     1501 tgctggacag cttcgtgccc ggcgccaacg ccgacctcct cgccgccctg ttcgaatgcg
     1561 tgccggtggc cgacacgccc cagcacctct acctgtgggg cgacgccggc agcgggcgca
     1621 gccacctgct gcggggcgtg gtcaccgccg ccgcccaggc cggccgaccc gcctgctacc
     1681 tgcgcgccgc cgacgtggtc gacacgctgc cggatgcccc ggcgctgctg ctggcggtgg
     1741 atgacgtgga gcgcctcggc gcggacgccc agatcgccct gttccgcgcc tgcaacgccg
     1801 cccgcggcca gcgcatgacc ctcgtcacca gcggcgccgt gccgccgcgg gatctgcccc
     1861 tgcgggaaga cctgcgcacc cgcatcggcc agagcctgat cttcgaggtc aaggccctcg
     1921 gcgacgacga gcgcgccgcc atcctcgccg cccacgccga cgcgcggggc atgcgcctgg
     1981 aggcggacct ggttcagtac ctgctgcgcc acgcgccgcg ggatctcaag agcctgctgc
     2041 gtaccgtgga cgccctcgac gccgcgtccc tggagcgcaa gcgcgccatc accctgcccc
     2101 tgctgcggga cgtcctgcag cacgtccccc cgaccgcctg aaccgccatg gaactcgtac
     2161 tcttcgacct ggacaacacc ctgctcgccg gcgactcgga tttcgagtgg gcccaattcc
     2221 tcatcgccaa gggcgtgctc gacaaggaag tctacgaggc ccgcaacctg gccttctacg
     2281 agcagtacaa ggccggcacc ctggacatct acgaattcct ggacttccag ctcctgcccc
     2341 tcgcccgcca cagccgcgag cagctcgacc tgtggcaccg ggaattcatg gacagccgca
     2401 tccgccccat gatgacgccc gccgcccgcg ccctggtgcg gcgccacctg gactcccgcg
     2461 cggtggtggc catcgtcacc gccaccaaca gcttcgtcac cggccccatc ggccgcgcct
     2521 tcgagattcc ccacctggtg gccaccgtgc cggcccagga gcacggcgtc ttcaccggca
     2581 agccccgcgg cacgccggcc ttcaaggcgg gcaagatcga gcgcgtggaa aactggctgg
     2641 aagccctcgg cctgcactgg ggcagcttcc gccgcagctg gttctacagc gactcccaca
     2701 acgacctgcc cctgctggag aaggtctccg acccggtggc cgtggatccg gacgacaccc
     2761 tgcgcgccca cgccaccgcc cgcggctgga acatcctcag cctgcgctga cggggccccg
     2821 gcggcggtcc gggcgccccg tcacagaccg ttggcgcgga accacgccag cagccgcgcc
     2881 cagccgtccg ccgcctccgc ggcccgatag ctgggccggt agtccgcgtg gaaggcgtgg
     2941 cccgcgtccg ggtaaagcac cagctcgccc cgcttgcccg ccgccgccag acgctcgcgc
     3001 atcgcctcca catcgctcac cgggatgccc tggtccttgg ccccgtagag gcccagcacc
     3061 ggcgcctgca gcgcctccac cacgtcgagc ggatgggtgg gcgtgttcgc cgacggcgtg
     3121 ccgtcgagcc gcccgtacca ggccaccgcc gccttcaggc cggcgtgccg ggctgcgaac
     3181 agccaggcga tgcgcccgcc ccagcagaag ccggtgatgc ccagccggcc gtcgccgccc
     3241 tgggtcagcg cccactcggc gcaggcctcc agatcgccca gcacctgggc gtccggcacc
     3301 ttcgccacga tgttcgcctg gatctccgcc acgctggcca gcgcggacgg gtcgccctgg
     3361 cgctggaaca ggttgggcgc gatggccaga tagcccgccc gtgccaggcg ccggcagacg
     3421 tcgcggatgt actcatggac gccgaagatc tcctgcacca ccagcaccac cggcacgggg
     3481 ccatcccccg ccgggcgcgc ccggtaggcc gccagcgcca cgccatccgg cgccacgatg
     3541 cgcacctcgc ccgccagcag gccatctgcc ggcgtgtgga tcacgtcctg gcccagagcc
     3601 ggacgcaccg acagggcaaa gcccgccgcc agggcggccc cgacgaaggt ccgccggccc
     3661 acggactggc cgggcacgag gctgtcaaaa tcgggatcgg tcacggtgtc ggtgtcgtgc
     3721 atggcgcgct cctggaggat ggggtcggtc gccagcatac gcgcaaatgg tgatgcccga
     3781 gaccgcgggg aacggggacg tcaccgacgg cacggcaggc gaaccggcct cccatcgaca
     3841 catcccgcag accggcgaac cggactgcgc cgcgccggcg cccgcaccgc gcagaccgcg
     3901 cggggaaacg gccgctggcg gacatcgcga aaaccgcgat ccgccgccct gcgtcaaccc
     3961 cgcgccgcgg ccggggcgcc ctggatgcgc gccaggcgca gaccgacgac gaagctcgcc
     4021 gccatcaccg tcaccacgac ggcgcccagc agcagctcct tgccctcgcc ccaggcctcg
     4081 atgcgcggcg agagcagccg cgccaggccg taggccgcca ccagcaggct catgcccgcc
     4141 accgccaggg ccatcacccg cgaggcaatg cgcgccacgc cgtccgcgcg ggcgatcagg
     4201 cgggcgatcc agaagccatt ggcgccgtcg gccaccagca tgcccagcac gaagcacccc
     4261 gccagcccgg ccgcgcgcag ggcgccacca ccgacgccgc tgccgcccgc cacggcgaac
     4321 agggccgcct ggctcagggt gtcgaaggac agggcgaaca gcgcgcccac cagggccacg
     4381 ccggcgggat gactgacctc ggcgaggcgg ccgagccagc gccctttcag gcctagcgga
     4441 cgcaccaccg catcccgggg cgcggccagc accgcaccga gattgagcac gcccagcccg
     4501 agcaggaaca ggatggagat gccggcgccg gacagctcca gccattccgg cgcctgccag
     4561 ccgcgggaca gcaggctcgc ccccaccgcc accgccagca ccacggcgcc gtggcccagg
     4621 gagaacaggg tgccgcagag gcgtgccagc cgcggccggc tacgggcatt gaagcgcgtc
     4681 aggccgtcga tggtggccag gtggtccgcg tcgaagccat gcttgagccc gagcaggaac
     4741 accagggcga cgaggctcgg gctgtcgagg ggcaggcttt ccatgggacg ggctccgcgg
     4801 cgtaggaaaa aactctgaag gtgcttacaa gaagcatgcc acggaatgaa gcccgtcgcg
     4861 acgggcaatg ggtgcgcggc gccggcaagt ccctttccgc ccgccccgcc acaccggcag
     4921 gacgacgacc gccccacctg cggcgcgtca gtccgccagg ggcaggcgca gggtgaagca
     4981 tgcgccgccg cggggatgat tgcccgccgt caggcgcccg ccgtggcgct ccacgatgcc
     5041 gtagctgatg gacaaaccca ggccggtgcc gctgcccacc ggcttggtgg tgaagaaggg
     5101 atcgaagatg tgaggcaggt ggcggtcctc gatgccgctg ccgttgtcgt gaaagtccag
     5161 cacgacttcg cgacgcgcgc ccgccgccgc gatctggccg cggatctcca gccgcgccgg
     5221 gtgctgcccg gcggtggcgt cgcaggcgtt ctgcaccagg ttcatcacca cctgctgcat
     5281 ctgccccgcg gagcccgtca cctgcagccc cggcggcagg tgcacagtca cccggaagcg
     5341 ttccggtgcg gccttgctta cccaggtgac ggagcgatcg atcaccgccg ccagatcgaa
     5401 gcgggcgttc tcctcccggt ccagcgcgga gaagcgcttg aggccgtcca cgatgtcgcg
     5461 ggtgcgctcc gccccttcga tggttccgtc gatgaggggc tccaggtcct tcaggatgcg
     5521 gtcgatgcgc aggcgctccc gcaggtccgc cagggcctcc gccccgacgc cgccatgcac
     5581 cgcggccagg tattcctcca gacgctgggc gtagcggcgc agggcgtggg tgttgccgag
     5641 gatgaagctg atggggttgt tgagctcgtg ggccacgccc gccaccagac gcccgagg
//
LOCUS       DAOQMM010000146         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_8307_length_5673_cov_46.438412, whole genome shotgun sequence.
ACCESSION   DAOQMM010000146 DAOQMM010000000
VERSION     DAOQMM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8307_length_5673_cov_46.438412"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..912)
                     /locus_tag="PLH85_16130"
     CDS             complement(85..912)
                     /locus_tag="PLH85_16130"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNB66146.1"
                     /translation="MNKHTFAALTLAGFGLAFALPGHAGSIAVDGNFSDWGISNNGTA
                     AGWKPTSGIFYTVEDQDNSNSGYLSPGWGGQAYDAEALYATWSTHANGQTYLDIALIT
                     GHDPSTVTNGSTSFGRGDFAIDFGRDGSWDYGILTADRSTTLHQGDVVATTNADWAKG
                     LWTAPGVYDPAHSQYITAVKTGTDVGDAALAISTVFTNMGVLGGNHWFYELEIPVTAF
                     GQHWTSSGPTEAFTLEWTMLCSNDIITLDPSRVPEPATAALLLGALGVLGAARRQRR"
     gene            complement(1084..1521)
                     /locus_tag="PLH85_16135"
     CDS             complement(1084..1521)
                     /locus_tag="PLH85_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNB66147.1"
                     /translation="MAITWILVANASLARLYANLGPNKGLQLIKELVHPQSRMKNADL
                     VTDRAGLLQAGSGHGAREPATPPKQNAARSFAQMLAKELYQGRSRNKFGRAILVAPPA
                     FMGMLNHEMDRNTAQMVSDRLEKDYTKTPEPALCGRLENCLFV"
     gene            complement(1624..2409)
                     /locus_tag="PLH85_16140"
     CDS             complement(1624..2409)
                     /locus_tag="PLH85_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNB66148.1"
                     /translation="MFAAFRRWRRQRAADRLTVTAAQWDAVEATLPFLAWLPADDRPA
                     LRRMALEFLADKEIHGAHGLQVTDAMLLSIALQACLPVLHIGLDAYAGWVGIVVYPGD
                     FVIPRQDVDEDGVVHEYDDEVLGEAWDGGPVLLSWFDGDGDDAPDGVNVVIHEFAHKL
                     DMGNGEVDGFPALPPGMSPQAWADAFRPAYEDFCRRVDAGEDTALDPYASQDPGEFFA
                     VLSEAFFETPGRLAAEYPAVYEQFSRYYRQDPRAQEAAASTKI"
     gene            2515..4719
                     /locus_tag="PLH85_16145"
     CDS             2515..4719
                     /locus_tag="PLH85_16145"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNB66149.1"
                     /translation="MVAVTHSISQSTSASSPQELLCEGLQPGDAVRVAEALAWAHSLY
                     GARTLGTGEPVWEHALGTALIAASLRLDADTRIAALCFAVGDVLDHPGDRIAEHFGAG
                     VAGLVDGLRRLNGLRVLTRLTALASAPEIRAQTEVLRKMLLAMVEDIRVVLLRVASRT
                     QTLRYFTDHPGPTRDAMARESLDIYAPLANRLGIWQLKWELEDLSFRFLEPDTYKRIA
                     KMLDERRVEREQFIVDAVERVKREVAAIGVEAEVYGRPKHIYSIYNKMRSKRLDFSQI
                     YDVRALRVLVHEVKDCYAVLGIVHQMWTPIPKEFDDYISMPKGNNYQSLHTAVYAGDG
                     RSLEVQIRTHDMHHHAELGVAAHWRYKEGSGAAAGAYDEKIALLRSLLSWRDEVTDAA
                     DWLEQFKRASLDDTIYVLTPQGRVIDLQRGATPIDFAYRVHTDLGHRCRGAKVNGQLV
                     TLNTPLESGQTVEIMSAKEGGPSRDWLNPHQAYVATPRARQKIKQFFSAQEEEELLVR
                     GRSVLTREMQREGFAQTNIDELATRLGFKTAEAMFIAAGRGELGPRAIQTALRGADAD
                     DTAPEPEVLIGRQHSANAGDTILIVGVGKLMTSLGRCCKPAPPDAIQGYVTRGRGVSI
                     HRIECRDFQELARAHPERLVAAQWGSAAAPKDTAVFPVDILVHARDRQGLLRDISEVL
                     SREKLNVIAVNTLSRKGAARMRFTIEVQGVQQIQRAISLIHEIKDVTDVERG"
     gene            4730..5593
                     /locus_tag="PLH85_16150"
     CDS             4730..5593
                     /locus_tag="PLH85_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB66150.1"
                     /translation="MTDRQPHHYAWLSIAAALFTIAFKALAWWLTGSVGLLSDALESF
                     VNLAGACFALWMILVTRLPPDRGHPFGHGKAEYFSSGFEGILILGAALAIIAAAAERL
                     LQPQPLESPGWGLVWSGVATAINFGVARTLARAGRQFNSIALQADARHLMTDVWTSVG
                     VILGVGLVAATGWLRLDPLIALAVGAQIVVEAVRLLRESIDGLMDRSLDGDALNRAEA
                     ALAPFRQRGIAFTALRTRRAGASAFVQVTVRVPGDWSVRSGHACLDDVEAALRTALPG
                     AEVITHLEPLS"
BASE COUNT          899 a         1959 c         1920 g          895 t
ORIGIN      
        1 ccaaaaaaaa gccggcccgg aggccggcaa attcctaggt gccagagaat gaaatcggtg
       61 gggcagcgcc tgcgcccgtg gggatcagcg gcgctgccgg cgtgccgcgc ccagcacgcc
      121 gagggcgccc agcagcaggg cggcggtggc aggttccggg acgcggctgg ggtcgagggt
      181 gatgatgtcg ttcgagcaca gcatggtcca ctcgagggtg aaggcctcgg tgggcccgct
      241 ggatgtccag tgctggccaa aggcggtgac cggaatctcc agctcgtaga accagtggtt
      301 gccgcccagg acgcccatgt tggtgaagac ggtggagatg gccagcgcgg catcgcccac
      361 gtcggtgccc gtcttcaccg cggtgatgta ctgggaatgg gccgggtcat agacgccggg
      421 tgcggtccac aagcccttgg cccagtcagc gttggtggtg gcgaccacgt cgccctgatg
      481 cagggtggtg gagcggtcgg cggtgaggat gccgtagtcc cagctgccgt cgcggccgaa
      541 gtcgatggcg aagtcaccgc ggccgaagga cgtgctgccg ttggtgacgg tgctcgggtc
      601 gtggccggtg atcagggcga tgtccaggta ggtctggccg ttggcgtggg tgctccaggt
      661 ggcgtagagc gcttccgcgt cgtaggcctg accgccccag ccggggctca ggtagccgct
      721 gttggaattg tcctggtctt ccaccgtgta gaagatgccg gaggtgggct tccagccggc
      781 ggcggtgccg ttgttggaga tgccccagtc gctgaagttg ccatccacgg caatggagcc
      841 ggcgtggccg ggcagggcga aggcgaggcc gaagccggcg agggtcaggg ccgcgaaggt
      901 gtgcttgttc atgattccgc accacttgaa atgtcgatgg cacggagctt aggcgcctga
      961 aatccgcatc aggcggtact tctccacgga atgcgggtgg gatgggggaa atgaaaaggc
     1021 ggtcgcgacc cgtgtcacgc ccgccttgaa gatgcgcggt gcccgcacgg ggcacccgtg
     1081 tgttcagacg aagaggcagt tctccaggcg accgcagagg gcgggctccg gcgtcttggt
     1141 gtagtccttt tcgagccggt cggagaccat ctgcgcggtg ttgcggtcca tctcgtgatt
     1201 gagcatgccc atgaaggcgg gcggggcgac gaggatggcg cggccgaact tgttgcgact
     1261 tcggccctgg tagagttcct tcgccagcat ctgggcgaag gagcgggcgg cattctgttt
     1321 gggcggggtg gcgggttcgc gggcgccgtg tccgctgccg gcctggagca ggcccgcgcg
     1381 gtcggtgacg agatcggcgt tcttcatgcg gctttgcgga tggaccagtt ccttgatgag
     1441 ctgcagtccc ttgttcggtc ccaggttggc gtacagacgg gccaggctgg cgttggcgac
     1501 cagaatccag gtaatagcca tgaatcgctc tccatagaat tgcaaaatgc cgtggacggc
     1561 tttctcactg tagatgcgat ccggccggca ttcaaagtga cgttgcggaa atgtgtcggc
     1621 ccgttaaatc tttgttgacg ccgccgcctc ctgcgcccgt ggatcctgcc ggtagtagcg
     1681 ggaaaactgt tcgtaaacag cagggtattc cgcagccagg cggcctggcg tttcgaagaa
     1741 ggcctcgctc agcacggcga agaactcgcc cgggtcctgg ctggcgtagg gatcgagggc
     1801 ggtgtcctcg ccagcgtcta cgcgtcggca gaagtcctcg taggcgggcc ggaaggcgtc
     1861 ggcccaggcc tggggcgaca tcccgggcgg cagcgcggga aagccgtcca cctcgccgtt
     1921 gcccatgtcc agcttgtggg caaattcgtg gatcaccacg ttgacgccgt ccggcgcgtc
     1981 gtcgccgtcc ccgtcgaacc aggacagcag caccggcccg ccgtcccagg cctcgccgag
     2041 cacctcgtcg tcgtattcat gcaccacgcc atcctcgtcc acatcctgcc gcggaatcac
     2101 gaaatccccc ggatagacca cgatgcccac ccagcccgcg taggcgtcca ggccgatgtg
     2161 gagcaccggc aggcaggcct gcagggcgat ggagaggagc atcgcgtcgg tgacctgcag
     2221 gccgtgggcg ccgtggatct ccttgtcggc gaggaattcc agggccatgc ggcgcagggc
     2281 cgggcggtcg tcggcgggca gccaggcgag gaagggcagg gtggcctcca ccgcgtccca
     2341 ttgggcggcg gtgacggtca ggcggtcggc ggcgcgttgg cggcgccagc ggcggaaggc
     2401 ggcaaacatg gtgcgatggt agccgaggcg ggaaccgggc ggcgggtgtg gagtccatca
     2461 ctgcggacga tccaggtcaa acccctgcca ttctggctgt aggataatgt cgtcatggtt
     2521 gccgttaccc attccatctc ccagtcgacc tccgcgtcat ccccccagga actgctctgt
     2581 gaaggccttc agcccggcga cgccgtgcgc gtggcggaag ccctcgcgtg ggcccattcg
     2641 ctgtatgggg cgcgcaccct gggcaccggc gaacccgtgt gggagcacgc cctcggcacg
     2701 gccctgatcg cggcttccct gcgcctcgac gcggacaccc gcatcgccgc cctgtgcttt
     2761 gcggtgggcg atgtgctcga tcatccgggc gaccgcatcg ccgagcactt cggcgccggc
     2821 gtggcggggc tggtggatgg cctgcgccgc ctcaacggcc tgcgcgtgct cacccgcctg
     2881 acagccctcg ccagcgcgcc ggagatccgc gcccagaccg aagtcctgcg caagatgctg
     2941 ctggccatgg tggaagacat ccgcgtggtg ctgctgcgcg tggcctcgcg cacccagacc
     3001 ctgcgctact tcaccgatca ccccggcccc acccgcgacg ccatggcgcg ggaaagcctg
     3061 gacatctacg ccccgctggc caaccgcctg ggcatctggc agctcaaatg ggaactggaa
     3121 gacctgtcct tccgttttct ggagcccgac acctacaagc gcatcgccaa gatgctcgac
     3181 gagcgccgcg tggagcggga gcagttcatc gtggatgcgg ttgagcgggt caagcgcgaa
     3241 gttgccgcca tcggcgtcga ggccgaggtc tatggccggc ccaagcacat ctacagcatc
     3301 tacaacaaga tgcgctccaa gcgcctggat ttctcccaga tctacgacgt gcgcgccctg
     3361 cgcgtgctgg tgcacgaggt caaggactgc tacgccgtgc tgggcatcgt gcatcagatg
     3421 tggacgccga tccccaagga gttcgacgac tacatctcca tgcccaaggg caacaactac
     3481 cagtccctgc acaccgccgt gtatgccggc gacgggcgct cgctggaggt gcagatccgc
     3541 acccacgaca tgcaccatca cgccgagctg ggcgtggccg cccactggcg ctacaaggaa
     3601 ggctccggtg ccgccgccgg cgcctacgac gaaaagatcg ccctgctgcg cagtctgctc
     3661 tcgtggcgcg acgaggtgac cgacgccgcc gactggctgg aacagttcaa gcgcgcctcc
     3721 ctggatgaca ccatctacgt gctcacgccg caggggcggg tcatcgacct gcagcggggc
     3781 gccacgccca tcgacttcgc ttaccgggtg cacacggatc tgggccaccg ctgccgcggc
     3841 gccaaggtca atggccagct cgtcaccctc aacacgcccc tggaaagcgg ccagaccgtc
     3901 gagatcatgt ccgccaagga aggcggcccc tcccgcgact ggctcaaccc gcaccaggcc
     3961 tacgtggcca cgccgcgggc ccggcagaag atcaagcagt tcttcagcgc ccaggaagaa
     4021 gaggagctcc tcgtccgcgg gcgcagcgtg ctcacccgcg aaatgcagcg ggaaggcttc
     4081 gcccagacca acatcgacga actggccacg cgcctcggct tcaagaccgc cgaagccatg
     4141 ttcatcgccg ccggccgcgg cgagctgggg ccccgcgcca tccagaccgc cctgcgcggc
     4201 gcagatgccg acgacaccgc gcccgaaccg gaagtcctca tcggccgcca gcacagcgcc
     4261 aacgccggcg acaccatcct catcgtgggc gtgggcaagc tgatgacttc cctcggccgc
     4321 tgctgcaagc ccgcgccgcc cgacgcgatc cagggctacg tcacccgcgg gcgcggcgtg
     4381 tccatccacc gtatcgaatg ccgcgacttc caggaactgg cccgcgccca cccggaacgc
     4441 ctcgtcgccg cccagtgggg cagcgccgcc gcgcccaagg acaccgccgt cttcccggtg
     4501 gacatcctcg tccacgcccg cgaccgccag ggcctcttgc gggacatctc cgaagtcctg
     4561 tcccgcgaaa agctcaacgt gatcgccgtg aacaccctca gccgcaaggg cgccgcacgc
     4621 atgcgcttca ccatcgaagt gcagggcgtg cagcagatcc agcgcgccat cagcctgatt
     4681 cacgagatca aggacgtcac cgacgtggag cggggctagg gcggcacgca tgacggaccg
     4741 ccagccccat cactacgcct ggctgtccat cgccgcggcg ctcttcacca ttgcgttcaa
     4801 ggccctcgcc tggtggctca ccggatccgt cggcctgctc tcggatgcgc tggaatcctt
     4861 cgtcaacctg gccggcgcct gcttcgccct gtggatgatc ctggtcaccc gcctgccgcc
     4921 ggaccggggc cacccttttg gccacggcaa ggccgaatat ttctccagcg gcttcgaagg
     4981 catcctcatc ctcggcgccg cgctggccat catcgccgcc gcggcggaac gcctgctcca
     5041 gccccagccc ctcgaatccc ccggctgggg cctcgtctgg tccggcgtcg ccaccgccat
     5101 caactttggc gtcgcccgca ccctcgcgcg ggccggccgc cagttcaatt ccatcgccct
     5161 gcaggccgat gcccgccacc tgatgaccga tgtgtggacc tccgtcggcg tcatcctcgg
     5221 cgtcggcctc gtcgccgcca ccggctggct gcgcctcgac cccctcatcg ccctcgccgt
     5281 gggcgcccag atcgtcgtcg aagccgtgcg cctgctgcgg gaatccatcg acggcctgat
     5341 ggaccgcagc ctcgacggcg acgccctcaa ccgcgccgaa gccgccctcg cgcccttccg
     5401 ccagcgcggc atcgccttca ccgccctgcg cacccgccgc gccggcgcca gcgccttcgt
     5461 ccaggtgacg gtccgcgtcc ccggcgactg gagcgtgcgc agcgggcacg cctgcctgga
     5521 cgacgtggaa gccgccctgc gcaccgcgct gccgggggcg gaggtcatca cccacctgga
     5581 gcccttgtcg taagggcgcg aggcgcgcgg gggcagggca gggggacgat ggggtgcgtc
     5641 cccggcaggc acgccgccct gcggcgtggc cga
//
LOCUS       DAOQMM010000147         5509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_8699_length_5509_cov_52.476348, whole genome shotgun sequence.
ACCESSION   DAOQMM010000147 DAOQMM010000000
VERSION     DAOQMM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8699_length_5509_cov_52.476348"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /gene="mnmE"
                     /locus_tag="PLH85_16155"
     CDS             complement(<1..1313)
                     /gene="mnmE"
                     /locus_tag="PLH85_16155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNB66151.1"
                     /translation="MARPPSELIAAIATAPGRGGIGVVRLSGSDLAPLARILGGGDTQ
                     PRTARFCRFRDAAGGLIDEGLLLYFPAPHSFTGEDVIELQGHGGPVVMQLLLGRCLEL
                     GARLAEPGEFTRRAFLNGKLDLAQAESVADLIEASTTAAARSAVRSLSGAFSSEVHAL
                     RDRLIDLRMLVEATLDFPDEDIDFLVEARAFERLAVMEGELAAILDRAKQGSLLRNGL
                     HVVLVGQPNVGKSSLLNRLAGEERAIVTDIAGTTRDALRETIQIEGIPLHITDTAGLR
                     ETSDEVEKIGIARTWQEIARADVVVRLVDVRAGLTEADAAIDARLPAGVERITVFNKV
                     DLSGRASERVEQGDIGVSLYLSAKAGEGVDLLRTELLRVAGWHRHGEDVILARERHLI
                     ALREARARVSAARDAGHALELLAEELRLAQDAINAITGEFTPDDLL"
     gene            complement(1294..2943)
                     /gene="yidC"
                     /locus_tag="PLH85_16160"
     CDS             complement(1294..2943)
                     /gene="yidC"
                     /locus_tag="PLH85_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNB66152.1"
                     /translation="MDTRRLILLLVFSFSMVMLWDGWQKHTHPAQPATAASGTAPASN
                     GVPTPTAATTVSAPGVPGVPQIASPAAAAPRMKVRTDVLVADVSAEGGDIVRLELTRH
                     KATGDAEHDYVLFDDGEKHLYAAQSGLIGNGLPTHKTRFALPAQERVLKDGEDAVSVR
                     LEAPATADGIRVSKLITFHRGSYVAEVSYEIANGSGAPIAPHAYFQFMRDGKPAEQAG
                     GFGVQTFTGPAFYTDAEKYQKVNFQDVTDKKAKFVTKAPDGWVAMVQHYFVAGWAPAA
                     GEREFFANALGGDLFAAGVIVPVAAIAPGQSGTVSVPLYAGPQEQSRLDQFAKGFDLV
                     VDYGWLTIIAAPLFWVLEWFHKLTGNWGWAIILVTIALKALFFPLSAASYKSMAKMKT
                     LGPRLQRMKEQFGDDKMKLNQAMMDLYKREKVNPLGGCLPILIQIPVFIALYWVLLGV
                     VEMRGAPWLGWIQDLSVKDPYYILPLIMGATSLVQQRLNPAPPDPIQAKVMMMMPILF
                     TFMFLWFPAGLVLYWVVNNCLSIAQQWQINRMIEAGGKAAE"
     gene            complement(3034..3243)
                     /gene="yidD"
                     /locus_tag="PLH85_16165"
     CDS             complement(3034..3243)
                     /gene="yidD"
                     /locus_tag="PLH85_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNB66153.1"
                     /translation="MKWLVLACLRTYRYAISPLLGRNCRFFPSCSEYALEAVERHGAL
                     HGGWLGLRRVCRCHPWSAGGYDPVP"
     gene            complement(3240..3614)
                     /gene="rnpA"
                     /locus_tag="PLH85_16170"
     CDS             complement(3240..3614)
                     /gene="rnpA"
                     /locus_tag="PLH85_16170"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNB66154.1"
                     /translation="MNGAATEDSGGPAWGFGPSLRLHKTDEFSSVFAFRRVVRGRYFA
                     LHYAPCRTDEPRLGLVVAKKLARRAVLRNLVKRIGRDVFRHAHASLPRYDVVLRLSAP
                     LASVTKRQMREDMLGLFGRLPR"
     gene            complement(3637..3771)
                     /gene="rpmH"
                     /locus_tag="PLH85_16175"
     CDS             complement(3637..3771)
                     /gene="rpmH"
                     /locus_tag="PLH85_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNB66155.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRMATRGGRKVIAARRAKGRHRLGV
                     "
     gene            4112..>5509
                     /gene="dnaA"
                     /locus_tag="PLH85_16180"
     CDS             4112..>5509
                     /gene="dnaA"
                     /locus_tag="PLH85_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNB66156.1"
                     /translation="MSEASSIPPSTAFWNHCLGCLEQELPAQQFKTWILPLRAEDLAE
                     GDAAGLRLVAPSNFHRQWLRDRYLRRIEELGEDYFGAPPVIEVGIAPAGGLRRPAAPP
                     PGPGSATSGIESVSTPATAAVGIATPVAPAVHTTRAETPLTAEAANYEKSRLNPDFTF
                     DTLVTGRANDLARAAAMQVAQNPGVSYNPLFIYGGVGLGKTHMIHALGNTVFKADPRK
                     VIRYVHAEDYYADVVRAYQQKSFDVFKRYYRSLDVLIIDDIQFFNNKNRTQEEFFHAF
                     NALTEAKKQIIITSDTYPKDVQGLEDRLISRFDWGLTVQIEPPELEMRVAILKKKAEA
                     ERISLDEDVAFLIAKNLRSNVRELEGALKKVLAFARFHGRDVTLEVAKDALKDLLNAH
                     NRQLTVEFIQKTVADYYKIKVADMHSKKRTRVIARPRQVAMALAKDLTPMSLPAIGEA
                     FGGRDHTTVLHACRTI"
BASE COUNT         1007 a         1944 c         1728 g          830 t
ORIGIN      
        1 agcaggtcgt ccggcgtgaa ctcgccggtg atggcattga tcgcgtcctg ggcgaggcgc
       61 agctcttctg ccaggagttc cagggcatgg ccggcgtcgc gggctgcgct cacccgggcg
      121 cgggcctccc gcagtgcgat cagatgacgc tcacgggcga ggatgacgtc ttctccgtga
      181 cgatgccacc ccgccacccg cagcagctcg gtacgcagca ggtcgacgcc ctccccggcc
      241 ttcgccgaga gatacaggct cacgccgatg tcaccctgct ccacgcgctc cgacgcccgt
      301 cccgacaggt ccaccttgtt gaacaccgtg atgcgctcta ccccggccgg cagccgcgca
      361 tcgatcgcgg cgtccgcctc cgtgagtccg gcgcggacat ccaccaggcg taccaccacg
      421 tcggcccgcg cgatctcctg ccacgtacgg gcgatgccga tcttctcgac ctcgtccgaa
      481 gtctcacgca gacccgccgt atcggtgatg tgcagcggga tgccttcgat ctggatcgtc
      541 tcgcgcagcg cgtcgcgggt ggtgccggcg atgtccgtga cgatggcgcg ctcttcgccg
      601 gcgaggcggt tgagcaggct cgacttgccc acgttgggct gacccaccag caccacatgc
      661 aggccgttgc gcagcaggct gccctgcttc gcccggtcga ggatggcggc cagttcgccc
      721 tccatcacgg ccaggcgttc aaaggcgcga gcctccacga ggaagtcgat gtcctcgtcc
      781 ggaaaatcca gggtcgcctc caccagcatg cgcagatcga tcaggcgatc ccgcagggca
      841 tgcacttcgc tggagaaagc gccactcagg gagcgcaccg ccgaacgggc cgccgcggtg
      901 gtggacgctt cgatgagatc ggcgacgctc tcggcctggg ccaggtcgag cttgccgttg
      961 aggaaggcac gacgggtgaa ttcgcccggc tccgccagac gggcacccag ctcgaggcag
     1021 cggccgagga gcaactgcat caccaccggg ccgccgtgcc cttgcagctc gatgacgtct
     1081 tcaccggtga aggagtgggg cgccgggaag tagaggagca gcccctcgtc gatcagcccg
     1141 cccgccgcgt cgcgaaagcg gcagaagcgg gcggtccggg gctgcgtgtc gcccccgccc
     1201 agaatgcggg caaggggcgc cagatcggat ccggacagcc ggaccacacc gatgccgccg
     1261 cgccccggcg cggtggcaat cgcggcgatc agctcactcg gcggccttgc caccggcttc
     1321 gatcatccgg ttgatctgcc actgctgggc aatggacagg cagttgttga ccacccagta
     1381 cagcaccaga ccggccggga accacaggaa catgaaggtg aacaggatgg gcatcatcat
     1441 catcaccttg gcctggatcg gatcgggcgg ggccggattg agacgctgct ggaccaggct
     1501 ggtggcaccc atgatgagcg gcaggatgta gtacggatcc ttcaccgaca gatcctggat
     1561 ccagcccagc cacggcgcac cgcgcatttc caccacgccc agcagcaccc agtacagggc
     1621 aatgaagacc gggatctgga tcaggatggg caggcagccg ccgagggggt tcaccttctc
     1681 gcgcttgtac aggtccatca tggcctgatt cagcttcatc ttgtcgtcgc cgaactgctc
     1741 cttcatgcgc tgcaggcgcg ggcccagggt cttcatcttg gccatggact tgtagctggc
     1801 ggccgacagg gggaagaaca gcgccttcag ggcgatggtg accaggatga tggcccagcc
     1861 ccagttgccc gtgagcttgt ggaaccactc cagcacccag aacagggggg cggcgatgat
     1921 ggtcagccag ccgtaatcca ccaccagatc gaaacccttg gcgaactggt cgagacggct
     1981 ctgttcctgg ggaccggcgt acagcggcac ggagacggtg ccgctctgtc ccggcgcgat
     2041 ggccgccacg ggaacgatca cacctgccgc gaacaggtcg ccgcccagcg cgttggcgaa
     2101 gaactcccgc tcgcctgcgg ccggcgccca gcccgccaca aagtagtgct gcaccatcgc
     2161 cacccagccg tcaggcgcct tggtgacgaa cttggccttc ttgtcggtca cgtcctggaa
     2221 attcactttc tggtacttct cggcgtcggt atagaacgcc ggaccggtga aggtctgcac
     2281 cccgaagccg ccggcctgtt ccgccggctt gccgtcgcgc atgaactgga agtaggcgtg
     2341 gggcgcgatc ggagcgccgc tgccgttggc gatctcgtag ctcacctcgg ccacatagct
     2401 gcctcgatgg aaggtgatca gcttgctgac gcgaatgccg tccgccgtag ccggggcctc
     2461 cagccgtacc gagaccgcgt cctcaccgtc cttcagcacc cgctcctggg ccggcagcgc
     2521 gaagcgcgtc ttgtgggtcg gcaggccgtt gccgatcaga ccactctggg ctgcatacag
     2581 atgcttctcg ccatcgtcga agagcacgta atcgtgctcg gcgtcacccg tggccttgtg
     2641 gcgcgtgagc tccagccgga cgatgtcgcc gccctcggcg gacacgtcgg ccaccagcac
     2701 gtcggtgcga actttcatgc gcggcgccgc agcggccgga gaggcgatct gcggcacgcc
     2761 cggcacaccc ggggcgctca ccgtggtggc ggccgtgggc gtgggcaccc cattgctggc
     2821 gggcgccgtc cccgacgccg ccgttgcggg ctgcgcggga tgggtatgct tctgccagcc
     2881 atcccacagc atcaccatgg aaaaggagaa cacgaggagg aggatcaggc gacgggtatc
     2941 catcggagtc aggtcgggga taagaggtga atggggaatg aggagacgtg agcaggcgag
     3001 ggttccacga tccggagcac agccaaccgc gcctcagggc acgggatcgt agcccccggc
     3061 actccacgga tggcagcggc acacccggcg caggcccagc cagccgccat gcagggcgcc
     3121 gtgacgttcg accgcctcga gggcgtactc ggaacagctc gggaagaaac ggcagttgcg
     3181 cccgagcaag ggactgatgg cgtagcgata ggtgcgcagg cacgccagca cgagccactt
     3241 catcggggca ggcgaccaaa caggccgagc atgtcttcgc gcatctggcg tttcgtcacg
     3301 gaagcaagcg gagccgacaa tcgtaacacc acatcgtaac gcggcaggct tgcatgggca
     3361 tgacgaaaca cgtcgcgccc gatgcgcttc accagattgc gcagcacggc gcggcgggcg
     3421 agcttcttgg caaccaccag cccgaggcgc ggttcgtctg tccggcaggg ggcgtaatgg
     3481 agggcgaagt agcgtccccg gaccacgcgc cgaaaagcaa aaacggatga gaactcatcc
     3541 gttttgtgca gtcgcaacga cggcccgaag ccccatgccg gcccgccgga atcttccgtc
     3601 gcggcaccgt tcatggcgcc gaccgcggaa gaagcctcag acgccgagac gatgacggcc
     3661 cttggcacga cgcgcggcga tgaccttgcg gccgccacgg gtggccatgc gaaccaggaa
     3721 gccgtgagtg cgcttgcgac ggacgacgga aggctggtaa gtacgtttca ttttgcttgc
     3781 ccggaaagtt gagtcaaacg cgtaatttca tacgttaacc ctctgtttgt caagctatat
     3841 tgaacagggc acgggaagcc gccccgcccg cgcccgacaa agggcagcgc ggacccggta
     3901 gccgcccgct tcagccggcc ccgccgagac ccgccatcaa aggctcaaca ccgcattccg
     3961 cttgtggata actcggcctc gcgcaggtac aattccggtt tggccggacg tccctcacga
     4021 ccagccgcac gccccgtcac cgcgacggcg cggacgacca taacgacaag tggcacccct
     4081 tcagccaccc gaaccgcacc gcaggaattt cgtgtccgaa gccagctcca tcccgcccag
     4141 cactgccttc tggaaccatt gtctcggctg cctcgagcag gaactgcctg cccagcagtt
     4201 caagacctgg atcctgcccc tgcgtgccga agacctcgcc gaaggcgacg ccgccggtct
     4261 gcgcctggtc gcgccctcca acttccaccg ccagtggctg cgcgaccgct acctgcggcg
     4321 catcgaggaa ctgggcgaag actacttcgg cgcgccgccg gtcatcgagg tcggcatcgc
     4381 accggccggc ggtctgcgcc gcccggcggc tccgccgccc ggtccgggct cggccacgtc
     4441 cggcatcgaa tccgtcagta caccggccac cgccgccgtg ggcatcgcca cgccggtcgc
     4501 cccggccgtc cacacgaccc gcgccgaaac gccgctcacc gccgaggccg ccaattacga
     4561 aaaatcgcgc ctgaacccgg atttcacctt cgacaccctg gtcaccggcc gcgccaacga
     4621 cctggcccgc gccgccgcca tgcaggtggc ccagaacccc ggcgtgtcct acaaccccct
     4681 gttcatctac ggcggcgtgg gcctgggcaa gacccacatg attcacgccc tcggcaacac
     4741 cgtcttcaag gccgacccgc gcaaggtgat ccgctacgtc cacgccgagg actactacgc
     4801 cgacgtggtg cgcgcctacc agcagaaatc cttcgacgtc ttcaagcgct actaccgctc
     4861 cctggacgtc ctgatcatcg acgacatcca gttcttcaat aacaagaacc gcacccagga
     4921 agagttcttc cacgccttca atgccctcac cgaggccaag aagcagatca tcatcaccag
     4981 cgacacctac cccaaggacg tgcagggcct ggaagaccgc ctgatctccc gcttcgactg
     5041 gggtctcacg gtgcagatcg agccgcccga gctcgagatg cgcgtcgcca tcctcaagaa
     5101 gaaggccgag gccgagcgca tcagcctgga cgaggacgtg gccttcctga tcgccaagaa
     5161 cctgcgctcc aacgtacgtg agctggaagg cgccctcaag aaggtgctgg cctttgcccg
     5221 cttccacggg cgcgacgtga ccctggaggt ggccaaggac gccctcaagg atctgctcaa
     5281 cgcccacaac cgccagctca ccgtcgagtt catccagaag accgtcgccg actactacaa
     5341 gatcaaggtc gccgacatgc actccaagaa gcgcacccgc gtcatcgccc gcccgcgcca
     5401 ggtggccatg gccctcgcca aggacctcac ccccatgagc ctgcccgcca tcggcgaggc
     5461 cttcggcggc cgcgaccaca ccaccgtgct gcacgcctgc cgcaccatc
//
LOCUS       DAOQMM010000148         5340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_9154_length_5340_cov_35.350804, whole genome shotgun sequence.
ACCESSION   DAOQMM010000148 DAOQMM010000000
VERSION     DAOQMM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9154_length_5340_cov_35.350804"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLH85_16185"
     CDS             <1..588
                     /locus_tag="PLH85_16185"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNB66157.1"
                     /translation="PEPVRAVAPPLPLPEIRPPPMRTVAAEPRPGPVLTRPTRAAAPD
                     TPVIVQAPTPPVPSSAPISAPAEASPRPAPVVAVTTPAEAAPAPHVDEAPDPALLERY
                     GRSLSGLLARQQNYPRLAALRGWEGEVQLRVTIARKGTIVATQVLRSSGFEVLDQNAI
                     ALVTAAAPLPRVPDALQGREFQIVVPVQYRLDKQS"
     gene            585..2027
                     /locus_tag="PLH85_16190"
     CDS             585..2027
                     /locus_tag="PLH85_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB66158.1"
                     /translation="MNTASPTAAPGARTAPTPDATTRALRWPRPRRLSLRLRISLILT
                     AFAALLILAGGALWIAEARNAIAEEVTSAHRVAVQWLTVSARAASDNDPAWSEPRLVA
                     YLQAAGRLRANQVELRDARGALLYRSPGSPYKAGRDAPPLFVAWMSPDLPARSLPAGH
                     LDIVIQPDASRAVLDLWDDLTAAAGRALLALIALFAGCWLAVDRALRPLGQVMAALGH
                     AGDGRFDTRLPEDGPRELARLAEAFNRMTLRLDQAVADNARLHHEQALGHVLHQRLEA
                     ERTVIARELHDELGQSITAVAALAGAILQRSDGQPTVQQSARTIRDVAAHMQDDVRAL
                     LTRLRPQRPAEETLDDALRSVLGAWQQRHPDIRLVTELFAGPAPLGDELTLVALRIVQ
                     ESCTNIVRHAQASQAYVRVLRQAGRLVVEVGDNGRGLQPASGQSGYGLTGMRERVAAV
                     RGELHISSPPGGGTRIRAVLPLPDSAPHDA"
     gene            2017..2658
                     /locus_tag="PLH85_16195"
     CDS             2017..2658
                     /locus_tag="PLH85_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB66159.1"
                     /translation="MTPELSARTVLLADDHAIVRMGFRLLLEGVGVHIVGEAESGEEA
                     QRLAAELRPDVLVMDLSMPGCGGLAALERILARSPAQAVLIASAHADAVTARRALKLG
                     ARGYLCKRSPPGELLTAVARIAAGQTYIDPGIAPELARGDKSADPAEALTDKEFHVFL
                     QLARGRSVNEVAATFHLSPNTVGTHLYHIKQKLGVQNMAELAMAAVRAGLIEV"
     gene            2681..3211
                     /locus_tag="PLH85_16200"
     CDS             2681..3211
                     /locus_tag="PLH85_16200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB66160.1"
                     /translation="MHPLSRIALASLAALFTPLIAAPALAARPVLHVKESIVIAAPPA
                     QVWAAAGDFGNLGWHPAVASTTIDQGENNRPGAVRTVTTKDGAKLVERLVARDDAARR
                     LSYTIIDSPLPVSDYRSTLRVLPDGAGSRVEWSSRFRRKAEKPLDGADDAGARRVIHS
                     IYSGGLGNLKATLEAH"
     gene            3224..>5340
                     /locus_tag="PLH85_16205"
     CDS             3224..>5340
                     /locus_tag="PLH85_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB66161.1"
                     /translation="MSHALRHPLPAEGIPGRRPSAPTPASRWRGLAGVLSLLLAACVL
                     FAPRLADAGDLSRDDLARRFQPPLHVGDKLRDIPAWPITSELEPEAGPVAYAFESIDL
                     APIPGFEGTPYNLLVSIDRKGNFLGVELLRQHEPVFLSGLGEGPLKDFLRQYEGKSLK
                     QEITVSSLYGNTRSGAGARRVVLDGVSKATASVRIANQTVLTAALAVARARLGFAAPV
                     DKGPTAEARADLYEARSFAQLVDDGAIGRLHLRNADVEKLFAGTDAAGLDADALARPD
                     DTFTDLYVAYLNAPTIGRALLGDAGHADLMRRLEPGQHAWWVATAGRDAFIDDQFTRG
                     TVPVRLAFSQAGGPVELRDLDLSPRPPAGAPPLNAALVLRVPPMSGVDPASPVRFELT
                     LNRSKGSILPQTFQKTVALDYQPPERLFVRPPRPLPEWLIAWKDRAAELIVIGLALAG
                     LTVVLARPRWMSISAPRLRVFRLGFLAFTLVYLGWYAQGQLSIVQITGAVKSLVAGQG
                     LKSFLYDPVSLLLIAFTAVSFVVWGRGTFCGWLCPFGALQEFAALLARALRLPALRMP
                     ARLGLVLERSRYAVLAALVLAAAFAPRLAEQGVEIEPFKTAITVGFNRDGPYVAWAVA
                     LLIAGGLYYKFFCRYLCPLGAAMTLGGRLRLLDWLPRRAECGQPCQTCRHRCTYDAIE
                     KSGAIRYDRCFQCLDCVGIYHDPG"
BASE COUNT          716 a         2419 c         1508 g          697 t
ORIGIN      
        1 ccagagcccg tccgcgccgt cgccccgccg ctgccgctcc ccgagatccg cccgcccccc
       61 atgcggaccg tcgcagcgga gccccgcccg ggcccggtcc tgacccgccc gacccgcgcc
      121 gccgcgcccg acaccccggt catcgtccag gcgcccacgc cgccagtgcc gtccagcgcc
      181 cccatcagcg cgccggccga agccagccca cgtcctgcgc cggtcgtcgc cgtcacgacg
      241 ccggccgaag ccgctccggc gccccacgtg gacgaagccc ccgatccggc cctcctcgaa
      301 cgctacggcc gcagcctttc cggcctcctt gcccgccagc agaactaccc gcgcctggcg
      361 gccctgcgcg gctgggaggg cgaagtgcaa ctgcgcgtca ccatcgcccg caaaggcacc
      421 atcgtcgcca cccaggtgct gcgctccagt ggcttcgagg tgctggacca gaacgccatc
      481 gccctcgtca ccgccgccgc gcccctgccc cgcgtgccgg atgccctgca ggggcgcgag
      541 ttccagatcg tcgtgccggt gcagtaccgg ctcgacaagc aatcatgaac accgccagcc
      601 cgaccgcagc ccccggcgcc cggaccgccc cgacacccga cgccaccacc cgcgccctgc
      661 gctggccccg cccgcgccgg ctcagcctgc gcctgcgcat cagcctcatc ctcactgcct
      721 tcgccgccct cctcatcctg gccggcggcg ccctgtggat cgccgaagcc cgcaacgcca
      781 tcgccgagga agtcacctcc gcccaccggg tcgccgtgca gtggctcacc gtctcggccc
      841 gcgccgccag cgacaacgac cccgcctgga gcgaaccgcg cctcgtcgcc tacctgcagg
      901 ccgccgggcg cctgcgcgcc aaccaggtcg aactgcgcga cgcccgcggc gccctgctct
      961 accgctcgcc cggttcgccc tacaaggccg gccgcgacgc cccgcccctg ttcgtcgcgt
     1021 ggatgagccc ggatctcccg gcccgcagcc tgcccgccgg ccacctggac atcgtcatcc
     1081 agcccgacgc ctcccgcgcc gtgctggatc tgtgggacga cctcaccgcc gccgccggcc
     1141 gcgccctgct cgccctcatc gccctgtttg ccggctgctg gctggcggtg gaccgggccc
     1201 tgcgccccct cggccaggtc atggccgccc tcggccacgc cggcgacggg cgcttcgaca
     1261 cgcgcctgcc ggaagacggc ccgcgcgagc tcgcccgcct cgccgaagcc ttcaaccgca
     1321 tgaccctgcg cctcgaccag gccgtcgccg acaacgcccg cctgcaccac gagcaggccc
     1381 tcggccacgt cctgcaccag cgcctcgaag ccgagcgcac cgtcatcgcc cgcgagctcc
     1441 acgacgagct gggccagagc atcaccgccg tcgccgccct ggccggcgcc atcctgcaac
     1501 gcagcgacgg ccagcccacc gtgcagcaga gcgcccgcac catccgcgac gtggccgccc
     1561 acatgcagga cgacgtgcgc gccctgctca cccgcctgcg cccccagcgc cccgccgaag
     1621 aaaccctcga cgacgccctg cgcagcgtgc tcggcgcctg gcagcaacgc cacccggaca
     1681 tccgcctcgt caccgaactc ttcgccggcc cggcgcccct gggcgacgaa cttaccctcg
     1741 tcgccctgcg catcgtgcag gaatcctgca ccaacatcgt ccgccacgcc caggccagcc
     1801 aggcctacgt ccgcgtgctg cgtcaggccg gccggctggt ggtggaggtg ggcgacaacg
     1861 gccgcggcct gcagcccgcg tccggccagt ccggctacgg cctcaccggc atgcgcgaac
     1921 gggtcgccgc ggtgcggggc gagctgcaca tcagcagccc acccggcggc ggcacccgca
     1981 tccgcgccgt cctgcccctc cccgactccg ccccccatga cgcctgaact cagcgcccgc
     2041 accgtcctcc tcgccgacga tcacgccatc gtccgcatgg gcttccgcct gctcctcgaa
     2101 ggcgtcggcg tgcacatcgt cggcgaggcc gagagcggcg aggaggccca gcgcctggct
     2161 gccgagctgc gccccgacgt gctcgtgatg gatctctcca tgcccggctg cggaggcctc
     2221 gccgcgctgg aacgcattct cgcccgcagc cctgcccagg ccgtactcat cgcctccgcc
     2281 cacgccgacg ccgtcaccgc ccggcgcgcc ctcaagctcg gcgcccgcgg ttacctgtgc
     2341 aaacgcagcc cgccgggcga actgctcacc gccgtcgccc gcatcgccgc cggccagacc
     2401 tacatcgacc ccggcatcgc gccggaactc gcccgcggcg acaaatccgc cgaccccgcc
     2461 gaagccctca ccgacaagga attccacgtc ttcctgcagc tcgcccgcgg gcgctcggtg
     2521 aacgaagtcg ccgccacctt ccacctcagc cccaacaccg tgggcaccca cctctatcac
     2581 atcaagcaga agctcggcgt gcagaacatg gccgagctgg ccatggccgc cgtccgcgcc
     2641 ggtctcatcg aagtctgacc tccaaaccaa ggaacgcccc atgcatcctc tgtcccgaat
     2701 cgccctcgcc agcctcgccg cgctgttcac ccccctcatt gcggcgcccg ccctggccgc
     2761 ccgccccgtg ctccacgtca aagagagcat cgtcatcgcc gcaccgcccg cccaggtgtg
     2821 ggccgcggcc ggcgacttcg gcaacctggg ctggcacccc gccgtggcca gcaccaccat
     2881 cgatcagggc gagaacaacc ggcctggcgc cgtgcgcacc gtcaccacaa aggacggcgc
     2941 caagctcgtc gaacgcctcg tcgcccgcga cgacgccgcc cgccgcctca gctacaccat
     3001 catcgactcg cccctgccgg tgagcgacta ccgctccacc ctgcgcgtcc tgccggacgg
     3061 ggcgggcagc cgcgtcgaat ggtcctcgcg cttccgccgc aaggccgaaa agcccctcga
     3121 cggcgctgac gacgcgggcg cccggcgcgt catccacagc atctacagcg gcggcctcgg
     3181 caacctcaag gccaccctcg aagcccactg acccgtcccc gccatgtccc acgccctccg
     3241 ccatcccctt cccgcggagg gcatccccgg ccgccgcccg tccgccccca cgcccgcgtc
     3301 ccggtggcgc ggcctggcgg gcgtcctgag ccttctcctc gccgcctgcg tcctgttcgc
     3361 gccccggctg gccgacgccg gcgacctgag ccgcgacgac cttgcccgcc gcttccagcc
     3421 gcccctccac gtgggcgaca agctgcgcga catccccgcc tggcccatca cttccgaact
     3481 cgaaccggag gccggccccg tcgcctacgc cttcgaatcc atcgacctgg cccccatccc
     3541 cggcttcgaa ggcacgccct acaacctcct cgtcagcatc gaccgcaagg gcaacttcct
     3601 tggcgtcgag ctcctgcgcc agcacgagcc ggtcttcctc tcaggcctcg gtgaaggccc
     3661 cctcaaagac ttcctgcgcc agtacgaagg caagagcctc aagcaggaga tcaccgtctc
     3721 cagcctctac ggcaacaccc gcagcggtgc tggcgcccgg cgcgtcgtgc tcgacggcgt
     3781 cagcaaggcc accgcctccg tgcgcatcgc caaccagacc gtcctcaccg ccgccctcgc
     3841 cgtggcccgc gcccgcctcg gcttcgccgc ccccgtggac aaaggcccca ccgccgaggc
     3901 ccgcgcggat ctctacgagg cccgcagctt cgcccagctc gtggacgacg gcgccatcgg
     3961 ccgcctgcac ctgcgcaacg ccgacgtgga aaagctcttc gccggcaccg acgccgccgg
     4021 cctcgacgcc gacgccctcg cccgccccga cgacaccttc accgacctct acgtcgccta
     4081 cctcaacgcc cccaccatcg gccgcgccct cctcggcgac gccggccacg ccgatctgat
     4141 gcgccgcctc gaacccggcc agcacgcctg gtgggtcgcc accgccggtc gcgacgcctt
     4201 catcgacgac cagttcaccc gcggcaccgt gcccgtgcgc ctcgccttca gccaggccgg
     4261 tggaccggtg gaactgcgcg acctggacct ctctcccagg ccccccgccg gcgccccgcc
     4321 cctcaacgcc gccctggtgc tgcgcgtccc gcccatgtcc ggcgtcgacc ccgccagccc
     4381 cgtgcgcttc gaactcaccc tcaaccgcag caagggcagc atcctgcccc agaccttcca
     4441 gaagaccgtc gcgctggatt accagccccc cgagcgcctg ttcgtccgcc cgccgcggcc
     4501 cctgcccgaa tggctcatcg cctggaagga ccgcgccgcc gagctcatcg tcatcggcct
     4561 cgccctcgcc ggcctcaccg tcgtcctcgc ccggccccgc tggatgtcca tctccgcgcc
     4621 gcgcctgcgc gtcttccgcc tcggcttcct cgccttcacc ctggtctacc tcggctggta
     4681 cgcccagggc cagctctcca tcgtgcagat caccggcgcc gtcaaatccc tcgtcgccgg
     4741 ccagggcctc aagagcttcc tctacgaccc cgtcagcctc ctcctcatcg ccttcaccgc
     4801 cgtcagcttc gtcgtctggg gccgcggcac cttctgcggc tggctgtgcc ccttcggcgc
     4861 gctccaggaa ttcgccgccc tcctcgcccg cgccctgcgc ctgcccgcgc tgcgcatgcc
     4921 cgcccgcctc ggcctcgtcc tcgaacgcag ccgctacgcc gtgctcgccg ccctcgtcct
     4981 cgccgccgcc ttcgcccccc gcctcgccga acagggcgtc gagatcgaac ccttcaagac
     5041 cgccatcacc gtcggcttca accgggacgg gccctacgtc gcctgggccg tcgccctcct
     5101 catcgccggc ggcctctact acaagttctt ctgccgttac ctctgccccc tcggcgccgc
     5161 catgaccctc ggcggccgcc tgcgcctgct cgactggctc ccccgccgcg ccgaatgcgg
     5221 ccaaccctgc cagacctgcc gccaccgctg cacctacgac gccatcgaaa aatccggcgc
     5281 catccgctac gaccgctgct tccagtgcct ggactgcgtg gggatctacc acgaccccgg
//
LOCUS       DAOQMM010000149         5327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_9206_length_5327_cov_42.130501, whole genome shotgun sequence.
ACCESSION   DAOQMM010000149 DAOQMM010000000
VERSION     DAOQMM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9206_length_5327_cov_42.130501"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PLH85_16210"
     CDS             complement(<1..1138)
                     /locus_tag="PLH85_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB66162.1"
                     /translation="MTVSPWRALANRRMLICVLTGFSSGLPLYLLLNLVPAWLKTEGL
                     SLKAIGAFALIQFPYTWKFLWSPLLDRFALPLLGRRRGWMAATQLALLAAVAWLGTLT
                     PGGQLGLVVGLTALIAFLSATLDIVLDAYRRELLPEIELGIGNAIHVNAYKLAGLVPG
                     SLSLVLADHLPWAQVFLVTSLFMLPGLLMTLLVGEPVAAAAAPRTLRDAVVAPFREFF
                     GRHGVGAALWVLAFIFLYKLGDSLCTALATPFYLDMGFSKTEIGLVAKNAGLWPSVIG
                     GLLGGLWMVRLGINRALWVFGFVQWAAIFGFAWLAWLGPAEADAARLGVLALVIGLEA
                     LAVGLGTTAFVAYMARTTHPAYTATQLALFTSLMAVPRTFINASA"
     gene            1289..1603
                     /locus_tag="PLH85_16215"
     CDS             1289..1603
                     /locus_tag="PLH85_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66163.1"
                     /translation="MYHVTVAFRADREYEFHVHGDEVAGLDTEAARDWLQQEFDALEC
                     TPSNPVGKILVLDVILNVAKYGGESRFAQGGAWARRFAACTGVVLDRPAIRVDVPGFT
                     VA"
     gene            1807..2235
                     /gene="fliW"
                     /locus_tag="PLH85_16220"
     CDS             1807..2235
                     /gene="fliW"
                     /locus_tag="PLH85_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNB66164.1"
                     /translation="MKITSPMLGEVDVSPERILDFPTGLPGFEGNTRFTLLHAEGDAP
                     KVFLLQSLDDTAVAFSVTTPDVLGLNYEFTLSDDEVAALQLASADDACVLLIVSRDED
                     TTAPLRASLMAPVVLNTTTRRGLQKIIGKVDTSVTLRAVG"
     gene            complement(2305..3516)
                     /locus_tag="PLH85_16225"
     CDS             complement(2305..3516)
                     /locus_tag="PLH85_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNB66165.1"
                     /translation="MIQFGLDRFLADPALRRPLAGRRVALLAHPASVTRDLTHALDAL
                     MALPDLHVTAAFGPQHGLRGDKQDNMMESPDYLDPRHGIPVFSLYGTVRRPTPAMMDS
                     FDVLLVDLQDLGCRIYTFITTLRYVLEAAAAHGKAVWVLDRPNPAGRPVEGSLLRPGW
                     ESFVGAGPLPMRHGLTLGELARWFVATLELDVDCQVIPMQGWQPEAAPGYGWPLGERS
                     WINPSPNAPNLSMARAYAGTVMLEGTTLSEGRGTTRPLELFGAPDVDAGRVIATMQAF
                     APPWLTGCRLRECWFEPTFHKHAGQLCQGVQIHVEDPAHYDHAAFRPWRVQALAFKAI
                     RRLWPDYPLWRDFPYEYEFDRLAIDLINGSPLLREWVDDPAAEAGDLDAWAVPDEQAW
                     CAARAPFLLYA"
     gene            complement(3524..4057)
                     /locus_tag="PLH85_16230"
     CDS             complement(3524..4057)
                     /locus_tag="PLH85_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HNB66166.1"
                     /translation="MTADRHALLCHPDAEPPAGVGVAASATRTADGGLQLEYRLRGDL
                     SGLDLPTAETVLPPERLWAYTCLEAFVATAGETAYVEFNFAPNGQWHRFAFSAYRQSV
                     TPPQTPAPQIALARDAAGLGLSVRLAASALPAGPLQVGLTAVLLDRAGRHSYWALRHP
                     PGRPDFHHRDGFALALP"
     gene            4288..4725
                     /locus_tag="PLH85_16235"
     CDS             4288..4725
                     /locus_tag="PLH85_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HNB66167.1"
                     /translation="MSLPPQRRFPDRLRQVALFELGGLLFITPPFAWLSGVPVRESVG
                     LLAVLALIAAVWNGVFNTLFDRLEGHFTGRTADRRPWLLRGLHAVLFEGILLLLTLPV
                     IVLWTGMNWWPALLADIGLALAYTGYAFVFNLGYDRAFPIAPE"
     gene            4820..>5327
                     /gene="asd"
                     /locus_tag="PLH85_16240"
     CDS             4820..>5327
                     /gene="asd"
                     /locus_tag="PLH85_16240"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767271.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNB66168.1"
                     /translation="MSDRLKVLPQYLLPKQALTVLAGTLAGAQAGGLSTAAIRAFIGR
                     YGVNMAEAEQPDPAAYACFNDFFTRALRAGARPLADADFLCPVDGAISQFGPITRDRI
                     FQAKGHDYSTHALVGGDRDLAIQFQDGDFATLYLSPRDYHRIHMPCDGRLTRMIHVPG
                     ALFSVNPTT"
BASE COUNT          838 a         1934 c         1858 g          697 t
ORIGIN      
        1 cggcggaggc gttgatgaag gtgcgcggca ccgccatcag gctggtgaac agggccagct
       61 gcgtggccgt gtaggcggga tgggtggtgc gcgccatgta ggcgacaaag gcggtggtgc
      121 cgagtcccac cgccagcgct tccagcccga tcaccagggc cagtacgccc aggcgcgccg
      181 cgtcggcctc ggccggtccc agccaggcga gccacgcaaa cccgaagatc gccgcccact
      241 gcacgaaacc gaacacccac agggcgcggt tgatgcccag ccggaccatc cacagcccgc
      301 ccagcaggcc gccgatgacg ctgggccaca ggccggcgtt cttggcaacg aggccgatct
      361 cggtcttgga gaagcccatg tccaggtaga aaggcgtggc cagcgcggta cacagggaat
      421 cgccgagctt gtagagaaag atgaaggcca gcacccacag ggcggcgccc acgccgtgac
      481 ggccgaagaa ctcgcggaag ggcgccacca ccgcgtcccg cagggtgcgc ggcgcggccg
      541 ccgcggccac cggctcaccc accagcaggg tcatcagcag gcccggcagc atgaacaggc
      601 tggtcaccag gaagacctgc gcccagggca gatgatccgc cagcaccagg gacaggctgc
      661 ccggcaccag gccggccagc ttgtaggcgt tcacgtggat ggcgttgccg atgcccagct
      721 cgatctccgg cagcagctcg cgccggtagg catccaggac gatgtcgagg gtcgccgaca
      781 ggaaggcgat cagcgccgtc agacccacca ccaggccgag ctgtccgccc ggcgtgagcg
      841 tcccgagcca cgccaccgcc gccagcaggg ccagctgcgt cgccgccatc cagccccgcc
      901 gccggcccag caggggcagg gcgaagcggt ccagcagcgg cgaccacagg aacttccagg
      961 tgtaggggaa ctggatcagc gcgaaggcgc cgatggcctt cagggacagc ccctcggtct
     1021 tcagccacgc cggcaccaga ttgagcagca ggtacagggg caggcccgac gagaagccgg
     1081 tgagcacgca gatcagcatg cgccgattgg ccagggcgcg ccatggggaa acggtcatgg
     1141 attcggaacg cagggcgggg tcaggcgggg gagcgccgga tgataacaat cggttacttg
     1201 attccggcac ccggccacag aaaaatgcag cgtccgccac gccgccaggc gcgtccggac
     1261 gccgccagaa tacaacgagg agacccgcat gtaccacgtc accgtcgcgt tccgggccga
     1321 ccgggaatac gaattccacg tccacgggga cgaagtcgcc ggcctcgaca ccgaggccgc
     1381 gcgggactgg ctgcagcagg aattcgacgc cctcgaatgc acgcccagca acccggtggg
     1441 caagatcctg gtcctcgacg tgatcctcaa cgtggccaag tacggtggcg aatcccgctt
     1501 cgcccagggc ggcgcgtggg cgcggcgctt tgccgcgtgc accggcgtgg tcctcgaccg
     1561 gccggcgatc cgcgtggacg tgcccggctt cacggtcgcc tgaatccggg cccgccgggg
     1621 catcgggcaa gcgcgtcgcc ctggccaaag cccctctaca aaacggggag aattgggggc
     1681 gattcccgcc tctctttccg cggtgggccg tccgccgccg gcgctatcgt gtctgctgaa
     1741 gatccgcggc ccgacgcccg gatcgcccgg ccgcgccggg ccaaccgacc gaaccggagg
     1801 gaaaccatga agatcaccag ccccatgctc ggcgaagtgg acgtgtcgcc cgaacgcatc
     1861 ctcgatttcc cgaccggcct gcccggcttc gagggcaata cccgcttcac cctgctccac
     1921 gccgagggcg acgcccccaa ggtcttcctg ctgcagagcc tggacgacac cgccgtggcc
     1981 ttctcggtca ccacgcccga tgtgctgggc ctcaactacg aattcaccct cagcgacgac
     2041 gaggtcgccg ccctgcaact ggccagcgcc gacgatgcct gcgtgctcct catcgtcagc
     2101 cgcgacgaag acacgacggc ccccctgcgg gccagcctga tggcgccggt ggtgctcaac
     2161 accaccaccc gccgcggcct gcagaagatc atcggcaagg tggacaccag cgtcaccctg
     2221 cgcgcggtcg gctgaacgcc cgttccgctc gcgtacacgg ggcgcctgcg ggcgccccgt
     2281 ggcgtttgcg cgccccggtc cgcctcaggc gtacagcagg aacggcgccc gcgccgcgca
     2341 ccaggcctgc tcgtccggca cggcccacgc atccaggtcg cccgcttcgg ccgccgggtc
     2401 gtccacccat tcccgcagca gcggcgagcc gttgatgagg tcgatggcga ggcggtcgaa
     2461 ttcgtactcg tagggaaaat cccgccacag gggatagtcc ggccacagcc ggcggatcgc
     2521 cttgaaggcc agggcctgca cccgccacgg acggaaggcg gcgtggtcgt agtgggccgg
     2581 atcttccacg tggatctgca cgccctggca gagctgcccc gcgtgcttgt ggaaggtggg
     2641 ctcgaaccag cactcccgta ggcggcagcc cgtcagccag gggggcgcga aggcctgcat
     2701 ggtggcgatg acgcgcccgg cgtccacgtc cggcgcaccg aagagttcca gcggccgggt
     2761 ggtgccgcgc ccttccgaga gggtcgtgcc ctccagcatc acggtgccgg cgtaagcccg
     2821 cgccatggac aggttggggg cgttggggct ggggttgatc cagctgcgct cgcccagggg
     2881 ccagccgtaa ccgggcgcgg cctcaggctg ccagccctgc atcgggatca cctggcagtc
     2941 cacgtccagc tccagggtgg cgacgaacca gcgcgccagc tcgcccaggg tgaggccgtg
     3001 gcgcatgggc agcgggccgg cgccgacgaa gctttcccag cccggccgca gcaggctgcc
     3061 ctccaccggc cggccagccg gattggggcg atccagcacc cacaccgcct tgccgtgggc
     3121 ggcggcggct tccagcacat agcgcagggt ggtgatgaag gtgtagatgc ggcaacccag
     3181 gtcctgcagg tccaccagca gcacgtcgaa gctgtccatc atcgccggcg tggggcggcg
     3241 caccgtgccg tacaggctga acaccgggat gccgtggcgc gggtcgaggt agtccggcga
     3301 ctccatcatg ttgtcctgct tgtcgccgcg caggccgtgc tgggggccga aggcggccgt
     3361 cacgtgcaga tccggcaggg ccatcagggc gtcgagggcg tgagtcaggt cgcgggtcac
     3421 cgacgccgga tgggcaagca gggccacccg ccgaccggcc agggggcggc gcagggcggg
     3481 atcggcgagg aagcggtcga ggccgaactg gatcatggcg aactcacgga agggcgaggg
     3541 cgaagccgtc gcggtgatgg aaatcgggcc gccccggcgg atggcgcagg gcccagtagc
     3601 tgtgccgccc ggcgcggtcg aggaggacgg cggtgagccc cacctgcagc ggcccggcgg
     3661 gcagcgcgga cgcggccagg cggacgctca ggcccagccc ggcggcgtcg cgggcgaggg
     3721 cgatctgcgg cgcgggcgtc tgcggcggcg tcacactctg gcggtaggcg ctgaaggcga
     3781 agcggtgcca ctgcccgttg ggggcgaagt tgaactccac gtaggccgtc tcgccggccg
     3841 tggcgacgaa ggcctccagg caggtgtagg cccacaggcg ttcaggcggc agcaccgtct
     3901 ccgccgtggg caggtccagc ccgctcagat cgccgcgcag gcgatattcg agctgcaacc
     3961 cgccgtcggc cgtccgcgtc gccgacgccg ccacgcccac cccggcgggc ggttcggcat
     4021 ccggatggca cagcagggcg tggcggtcag cggtcatggg ggctctcgga acgggccgga
     4081 cacggcccgg cgcgcagtgt agccgatcgg gccggggcgc cgggcgatga ggccgtggcc
     4141 gcatcgcccg cgcccgcacg cgacaacaca accgcctgac aggcccttga ccggggatct
     4201 accggtaggt accaacgaac gccgccgtgc ggacacaccg gcacgcgcct gacgtagact
     4261 gacgcgcccg cgcactccgc ctcacccatg tccctcccgc cccaacgccg cttccccgac
     4321 cgcctccgcc aggtcgccct gttcgagctc ggcgggctgc tcttcatcac gccgcccttc
     4381 gcctggctgt ctggcgtgcc ggtgcgggaa tcggtcgggc tgctggcggt gctggccctc
     4441 atcgcggcgg tgtggaacgg cgtgttcaac accctcttcg accgcctcga aggtcatttc
     4501 accgggcgca ctgccgaccg ccggccgtgg ctgctgcgcg ggctccacgc ggtgctcttc
     4561 gagggcatcc tgctgctcct gaccctgccg gtgattgtcc tgtggaccgg catgaactgg
     4621 tggccggcgc tgctggcgga catcggtctg gccctggcct acacgggcta cgccttcgtc
     4681 ttcaatctgg gctacgaccg ggcctttccc atcgccccgg agtgagcgcc gcggcacccg
     4741 cgccggccgg tataatcgcc gcccgacccc ccggtcggcc cgcgcccgtg gcgcgtcccc
     4801 gcctgctgga gattccgccg tgtccgaccg cctcaaagtg ctgccccagt acctgctgcc
     4861 caagcaggcc ctcaccgtgc tcgccggcac actggcgggc gcacaggcgg gcgggctgag
     4921 caccgcggcg atccgcgcct tcatcggccg ctacggggtg aacatggccg aggccgagca
     4981 gccggatccg gcggcctacg cctgcttcaa cgacttcttc acgcgtgccc tgcgggccgg
     5041 cgcgcggccc ctcgccgacg cggatttcct gtgcccggtg gatggcgcca tcagccagtt
     5101 cggcccgatc acccgagacc gcatcttcca ggccaagggt cacgactact ccacccacgc
     5161 cctggtgggc ggcgaccggg acctggcgat ccagttccag gacggcgact tcgccacgct
     5221 ctacctcagc ccgcgggact accaccgcat ccacatgccc tgcgacggcc gtctgacgcg
     5281 gatgatccac gtgcccggcg cgctgttctc ggtcaatccg accaccg
//
LOCUS       DAOQMM010000150         5282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_9343_length_5282_cov_44.169313, whole genome shotgun sequence.
ACCESSION   DAOQMM010000150 DAOQMM010000000
VERSION     DAOQMM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9343_length_5282_cov_44.169313"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5282)
                     /locus_tag="PLH85_16245"
     CDS             complement(<1..>5282)
                     /locus_tag="PLH85_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HNB66169.1"
                     /translation="TGALTLNAGDVDNTGGLIAAQQNTTIGTAVLNNSGGDLRSGGKL
                     TLSATGTVTNAGGRIASAADLTLSASTLDNTSGRMGAIGGNLTIETTSSPLNNASGHL
                     EAAGLARVSVKGFNNQGGLLTASSIELDTDGHALDNTSGRLIASGRGDVDRFDIRSGP
                     LDNRGGLIQAGRRLRVDTGAYGLSNVATQISGGLVSQGTLDILSAAVDNGAGFIGAQG
                     AMTLLAGMFANTQGGQVIGGDRIALSATTLDNRGGIVQGVGDVTLHLADLLDNRNSLI
                     RAGGTLAFSADRLLNSDTQNAALGLEGSAIRGEVRLIDNTGGAIRATGDLSLSSHGHI
                     LNIGGSMSAGGTLSLHDPAAVRSLTVTQTGGTLLAGRLLDIDADSLGGDGELLSNGDL
                     RIRVQQDYTHTGHLQAVGDARLETAGVLVNRAAPVAGGTLSLQGASVDNQAGAILNAG
                     RLELRATDSHTFTNRGLIDSSETFIDAITLNNLGTGRIYGDHLAITAVTLNNLAEGGT
                     APVLAARDRLDLGVGTLTNAEGALIFSAGDLALGGALDASRRATGQGTALVNSSATVE
                     ALGNMDLRVARLDNLNAHYTTVLETVSQTHVEEYQGKDAIKRYLPGSPDVYIYNDESD
                     YLHTPDGNYEEWSIYSYDRTVREERLLTSAPARIVAGGRITLTSDRVLNDRSQILAGG
                     LLDGTIGTLSNVSGIGQRIFSDIGTVTNKWREHPSGRDYTGSATHDYAPPDTVEGIDL
                     VPAAFQSLAATTETGLSLDPAKVQVSNSSIQTAGTVRPGSLTQADVIRDTAIDPLSAR
                     AADRADAIAAGPDAIVPSGRPPAATPGLSVTSAPSSALSPVSGAPGGDHAAALSIPTA
                     ASAQIPPPAGGEATPLSVVASPTGDHGTFPALPAGRPIDGLTGATANPIASPSAERWT
                     PGPQDIPTAHAEVSTRSAQARPADPLPAPSATHSPAPRPRSVTPILQVAATTASGTAS
                     VVRSGGVDVAIPSNSLFRAHPEVNGRYLVETDPAFTRYEAWLGSDYLLQALAMDPATT
                     QKRLGDGFYEQKLIRDQVAQLTGRRFLDGYASDEAEYRALMDAGLTVAKDWNLRPGVA
                     LSAAQMALLTRDMVWLVEQSVQLPDGSIQKVLVPQVYVRVRDGDLSASGALVAGRDVQ
                     LDVRGELMNQGTISAQGTARLSADTLRNLNGHIVGGDLTLQATGDLLNRGGELRGDTR
                     LSVAAGRDLIVESTTRSQDNDQGSRTHIDHLASLSVTGPNGALTALAGRDLTLSGATV
                     QSAGSVTLAAGNTVNLGTVGESSAHRIVWDANNWRTDRSTREVGTTVGAQGKVQMLAG
                     QDIALRAATVTSQNGAIALSAGRDVQILAGQSLDQVDAAHQHTSRGFLSSKTITTRDQ
                     VNLLGVQGSTLSANTVTVEAGRNVQITGGQLVSDQGTRIAAAGDLNIDAATETRGERH
                     LAATRSSGLLRSGLGVTLGSRELSVDGLTDSTRAVASVVGSTAGSLQIQAGKALRLTG
                     SDLQAPSGDIALQAPRIDIAEARETLHTQTETRFRQSGITVAVSTPALTALQTAQQMN
                     RAASQTADPRMKALAAASTALSAYSAGQAIVAGQGVTLPDGRTGQIVTGKDATGEPIS
                     RDATTADRIGGINITVSAGSSRSVSTQVVDSDTGRSSLVQAGRGVAITARGGANDGTL
                     NVRGSTLSAGTTLTLDADNTLKLLAAQNRTTQTSANQSTSGSIGVTAGSGGVGVTLSA
                     SSGRGHSDGTDLSQLNTHLQAGQDIDLRSGGDTT"
BASE COUNT          660 a         1631 c         2054 g          937 t
ORIGIN      
        1 cgtggtgtcg ccaccgctgc gcaggtcgat gtcttgcccg gcctggaggt gggtgttgag
       61 ctggctgagg tcggttccgt cgctatggcc gcggccgctt gacgcgctga gggtgacgcc
      121 cacgccgccg ctgcccgctg tgacgccgat cgagccgctg gtggattggt tcgcggaggt
      181 ctgtgtcgtc cggttctggg cggcgaggag cttgagggtg ttgtccgcat ccagtgtcag
      241 ggtggtgccg gcggagaggg tgctgccgcg gacgttcagg gtgccgtcgt tggcgccgcc
      301 gcgcgccgtg atcgccacgc cgcggcccgc ctgcacgaga ctgctccgtc cggtgtcgct
      361 gtcgacgacc tgggtgctga cgctgcggct gctgccggcg ctgacggtga tgttgatgcc
      421 ccctatccgg tcggcggtgg tggcgtcccg gctgatgggc tcacctgtgg cgtctttgcc
      481 agtcacgatc tgaccggtgc ggccgtcggg cagggtgacg ccctgaccag cgacgatggc
      541 ctgtcccgcg gaataggccg agagggcggt gctcgcggcg gccagggcct tcatgcgcgg
      601 atctgcggtc tggctggccg cgcggttcat ctgctgggcg gtctgcaggg ctgtcagtgc
      661 cggagtcgac acggcgacgg tgatgccgct ctggcggaaa cgggtttcgg tctgggtgtg
      721 gagcgtctcg cgtgcctcgg cgatgtcgat gcggggcgcc tgaagggcga tgtcgccgga
      781 gggcgcctgg aggtcgctac ccgtgaggcg caaggcctta ccagcctgga tctgtaggct
      841 gccggcggtg ctgccgacca cgctggccac tgcgcgggtg ctgtcggtga ggccgtctac
      901 actgagttcg cggctgccga gggtgacgcc cagaccgctg cgcagcaaac cgctgctgcg
      961 ggtggcggcc aggtgtcgtt cgccgcgggt ttcggtggcg gcatcgatgt tcaggtcgcc
     1021 ggctgcggcg atgcgggtgc cctggtcgct gacgagctgg ccgccggtga tctggacatt
     1081 gcggcccgct tcgacggtga cggtgttggc cgacagggtg ctgccctgca cgccgagcag
     1141 attgacctgg tcgcgggtcg tgatggtctt ggacgaaagg aagccgcggg aggtgtgctg
     1201 gtgggcggcg tccacctggt cgagggactg gccggcgagg atctgcacgt cgcgcccggc
     1261 ggacagcgca atggccccgt tctggctggt gacggtggcg gcgcgcaggg cgatgtcctg
     1321 ccccgcgagc atctgcacct tgccctgagc gccgacggtg gtgccgacct cccgggtgct
     1381 gcggtcggtg cgccagttgt tggcgtccca cacgatgcga tgggcgctgc tctcgccgac
     1441 ggtgcccagg tttacggtgt tgccagcggc aagggtcaca ctgccggcgc tctgcacggt
     1501 cgcgccgctc agggtgagat cgcggccggc cagggcggtc agcgcgccgt tcgggccggt
     1561 aaccgacagg ctggcgagat gatcgatgtg ggtccggctc ccctggtcgt tgtcctggct
     1621 gcgggtcgtg ctttcgacaa tcaggtcgcg gcctgcggcc actgagaggc gcgtatcgcc
     1681 ccggagctcg ccaccccggt tgagcagatc gcccgttgcc tgcagcgtga ggtcgccgcc
     1741 gacgatgtgg ccattgaggt tgcgcagggt gtccgccgag aggcgggccg tgccctgggc
     1801 cgagatcgtg ccctgattca tcaactcgcc ccggacgtcg agctggacat cccgaccggc
     1861 cacgagggcg ccactggcgc tgaggtcgcc gtcccgcacg cgcacataga cctggggcac
     1921 gagcaccttc tggatcgagc cgtcgggcaa ctggacggac tgctcgacga gccagaccat
     1981 gtcgcgggtg agcaaggcca tctgcgcggc gctcagggcg acacccgggc gcaggttcca
     2041 gtccttggcg accgtgaggc cggcgtccat cagggcacgg tactccgcct cgtcgctggc
     2101 gtagccgtcg aggaagcgcc ggccggtgag ctgcgccacc tgatcgcgga tcagcttctg
     2161 ctcgtagaag ccgtcaccga gtcgcttctg ggttgtggcc gggtccattg ccagcgcctg
     2221 cagcaggtag tcggaaccga gccaggcctc gtagcgggtg aaggccgggt ctgtttccac
     2281 gagatagcgg ccattcacct ccgggtgggc gcggaacagg ctgttgctcg ggatggccac
     2341 gtcgacgccg ccgctgcgga ctacgctggc ggtgccgctg gcagtggtgg ccgcaacctg
     2401 caggatgggg gtgaccgacc gggggcgggg ggccggggag tgggttgcag acggcgccgg
     2461 gagaggatcg gcggggcggg cctgtgcgga ccgggtcgag acctctgcgt gggcggtggg
     2521 gatgtcctgg gggccgggcg tccaccgttc ggctgacgga ctggcaatcg ggttcgctgt
     2581 cgcaccggtg agcccgtcga tggggcgacc cgccggcagg gcagggaagg tgccgtgatc
     2641 gccggttggg gaggccacga ccgatagggg cgtggcctcg ccgccagcag ggggcgggat
     2701 ctgggcggac gctgccgtcg ggatggagag ggccgccgca tgatcgcccc ctggcgcccc
     2761 cgataccggc gagagcgcgc ttgacggggc ggacgtgacc gaaaggccag gcgtcgcggc
     2821 agggggacgt cccgacggga cgatggcatc cggaccggcc gcgatggcgt ccgcgcgatc
     2881 cgccgcgcgg gcactgaggg ggtcgatggc ggtgtcacgg atgacatccg cctgcgtgag
     2941 gctgccgggg cgcacggtcc cggccgtctg gatcgacgag ttcgaaacct gcaccttggc
     3001 gggatcgagc gacaggcctg tctccgttgt ggccgcgagg ctctggaagg cggcggggac
     3061 gaggtcgatg ccctcaacgg tgtcgggcgg tgcgtagtcg tgggtggcgc tgccggtgta
     3121 gtcgcggccg ctaggatgtt cccgccactt gttggtgacc gtaccgatgt cggagaagat
     3181 gcgctgaccg atgccgctga cgttgctcag ggtgccgatc gtcccgtcga ggagtccgcc
     3241 ggcgaggatc tggctgcggt cgttgaggac gcggtcggac gtcagggtga tgcggccgcc
     3301 ggcaacaatg cgcgccgggg cgctggtgag cagccgttct tcccggacgg tccggtcata
     3361 gctgtagatg ctccattctt cgtagttgcc gtccggcgtg tgcaggtagt cagactcgtc
     3421 gttgtagatg tagacgtccg ggctgcccgg cagatagcgc ttgatggcgt ccttgccctg
     3481 gtattcctcc acgtgggtct ggctcacggt ttccaggacg gtcgtgtaat gggcgttgag
     3541 gttgtcgagg cgggccacgc gcagatccat gttgcccagc gcttcgacgg tggcgctgga
     3601 attgacgagg gccgtgcctt ggccggtggc gcggcggctg gcatccaggg cgccgccgag
     3661 ggccagatcg ccagcgctga agatgagggc gccttccgcg ttggtgaggg tgcccacgcc
     3721 cagatcgaga cggtcccggg cggccagcac tggcgcggtg ccgccttcgg ccagattgtt
     3781 cagcgtgacg gccgtgatcg cgaggtggtc gccatagatc cggcccgtgc ccaggttgtt
     3841 gagggtgatc gcgtcgatga aggtctcgct gctgtcgatc agcccgcggt tggtgaaggt
     3901 gtggctgtcg gtggcgcgca gttcgagccg gccagcgttc aggatggcgc ccgcctggtt
     3961 gtcgacgctg gccccctgca ggctgagggt gcctccggct accggggcgg cgcgattgac
     4021 gagcacgccg gcggtctcga ggcgggcgtc gccaacggcc tggaggtgcc cggtgtgggt
     4081 gtagtcctgc tggacgcgga tgcgcagatc gccgttggac agcagttcgc cgtcgccgcc
     4141 caggctgtcg gcgtcgatgt ccagcaggcg tccggcaagc agggtcccgc ccgtctgggt
     4201 gacggtcagg ctgcggacgg cggcgggatc gtgcaggctg agcgtgccgc ctgccgacat
     4261 gctgccgccg atgttgagaa tgtggccgtg actgctgagg ctcagatcgc cggtcgcgcg
     4321 gatggcgccc cccgtattgt cgatgaggcg gacttccccc ctgatggcgc tgccctccag
     4381 accgagcgcg gcgttctgtg tgtccgagtt gaggagccgg tcggcggaga aggcgagcgt
     4441 tcccccggcg cggatcaggc tgttccggtt gtccagaagg tcggcgaggt gcagcgtgac
     4501 gtcgccgacg ccctggacga tgccgccccg gttgtccaga gttgtggcgg acagcgcgat
     4561 gcggtcgccc ccgatgacct gtccgccttg cgtgttggca aacattcctg caaggagtgt
     4621 catcgcgccc tgggcaccga tgaaaccggc cccgttgtcc acggcagccg agaggatgtc
     4681 gagcgtcccc tggctgacca gaccgccgct gatctgtgtg gcgacattgc tgagcccgta
     4741 cgcgccggtg tccacgcgca ggcggcgacc cgcctggatc agtccgccac ggttgtccag
     4801 ggggccgctg cggatgtcga atcggtcgac gtcgccgcgt ccggaggcga tcaagcgacc
     4861 ggatgtgttg tcgagcgcgt gcccgtctgt gtccagttcg atgctggagg cggtgagcag
     4921 gccgccctga ttgttgaagc ccttcacgct cacgcgggca agtcccgcgg cttccaggtg
     4981 gccggacgcg ttgttcagcg gactgctcgt cgtttcgatc gtcagatttc cgccgatggc
     5041 ccccatgcgc ccggacgtgt tgtcgagcgt cgatgcggac aaggtcaggt ccgcggctga
     5101 agcaatgcgg ccgccggcgt tggtgaccgt gccggtggcg ctgagcgtga gcttgccgcc
     5161 gctgcggagg tcgccgcccg agttgttcag cacggcggtg ccgatcgtgg tgttctgctg
     5221 ggcggcaatg agtccacccg tgttgtccac gtcacccgca ttcagcgtga gcgcccccgt
     5281 tg
//
LOCUS       DAOQMM010000151         5004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_10162_length_5004_cov_34.879168, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000151 DAOQMM010000000
VERSION     DAOQMM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10162_length_5004_cov_34.879168"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1087
                     /locus_tag="PLH85_16250"
     CDS             41..1087
                     /locus_tag="PLH85_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB66170.1"
                     /translation="MQIHDLHQRLADLGAKPAHIQRILRAWTHAQPLDSGPTRRGPAL
                     PKAVMEALPALEAELAGLARLRSEHPGEDGSARLLVSLPDGHTVESVLLLRDGLCVSS
                     QVGCAVGCVFCMTGRDGLLRQVGSAEIVAQVVLARRLRPVKKVVFMGMGEPAHNLDNV
                     WDAIQLLGSAGGIAHKNLVFSTVGDRRVFERLPEGPVKPALALYLHTTRADLRAELLP
                     RAPRIDPAELVELADAYARATGYPIQFQWTLLDGINDTPEELDGLVRLLAGRYAILNM
                     IPYNSVPDLPYRRPAQARAAEIARSLHRRGVLTKLRTSAGQDVDGGCGQLRARSIAGE
                     TPVRVLRRRGPDGA"
     gene            complement(1106..2470)
                     /locus_tag="PLH85_16255"
     CDS             complement(1106..2470)
                     /locus_tag="PLH85_16255"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB66171.1"
                     /translation="MNTLWFSLRRRLLGLLLGGVSVAWGVTMVFSYVDAHHEVDELFD
                     AQLAQAGQTLLVLADQGGGRAVSRMEEAVHKYQRRLRFQIWDADGQLAMRSETAGTAP
                     LTPIDGFSETEDGAGHWRHFSQWNSARRLQVQVSEDHRIRDDLIAHIAWRIVLPALFG
                     LPLLGLGVWLATRRGLASLDVVAGQIASRAPEQLQAVSPASAPEEIRPMLEALNGLFR
                     RVDSALEAERRFTADAAHELRTPLAALQTQLQVALRARDEAERARSLAQIQSGLARAA
                     HLVEQMLQLARLDPESGLPDPRAVDLAALAEDVCAELGSAILARGLEFELAAEPGCVV
                     FGQAEWLRVLLRNLVDNALRYTPEGGRLRVGVARAAEGPGGGAIVLGVEDDGPGIPPG
                     QRESVLARFVRLEAGSAPGSGLGLAIVARIVDLHGARLALGSGAGGRGLRVEVRWPAP
                     TPTG"
     gene            complement(2467..3123)
                     /locus_tag="PLH85_16260"
     CDS             complement(2467..3123)
                     /locus_tag="PLH85_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB66172.1"
                     /translation="MRILLAEDDPQLGDGLTVGLRQAGFTVDWVRDGQAADVALQAEA
                     FDLLVLDLGLPRLGGLDVLARVRARGQAVPVLILTARDAVGDKVAGLDGGADDYLVKP
                     VDLDELAARIRALIRRSAGRSAPLLVWGELVLDPAARRLTRAGEPVELSGREFALLQM
                     LLEGAGRVLTRSQLEQSLYGWGDEPDSNALEVHIHHLRKKLGSELIRTLRGVGYTIPR
                     "
     gene            complement(3156..4451)
                     /locus_tag="PLH85_16265"
     CDS             complement(3156..4451)
                     /locus_tag="PLH85_16265"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB66173.1"
                     /translation="MSEPLPRLAIACQGGGAHTAFTAGALSYLLLAFEHFRRQPGGRV
                     PFQLTGLSGTSGGAITAAMAWSDPGAGGWAEGAQRVLRFWNRNKASLQFQDKDPWWWG
                     EFFTNASARWLANFEDFGPTVSWPPNPLTSTLTQDRMRTDIRAALDLPPEADAIRGRA
                     GVDLYVGAVDVINRDAVSPGSAFRTFPATAGDALHVDELLASACIPELFFAQKLKIRS
                     GRHRESYFWDGLYSQNPPLTNFFCNREAEHKPDLVWIVQINPSSYSGRAPMTPSQIDD
                     RRNELAGNLSLGQELRSIATVNQIAAELDALRAQLPADDAALRAPLFSRYKTVTVAFL
                     PLANEDDSGRPLAAYTLDHASKMNRDPAFIDALIAEGIATAHTLAHAGQLVRPACMTG
                     ERHARHFPNPSWPAPAELVPLLDDPVLAAQWRALAAGIA"
     gene            4651..5001
                     /locus_tag="PLH85_16270"
     CDS             4651..5001
                     /locus_tag="PLH85_16270"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB66174.1"
                     /translation="MSADALSATFAALADPTRRAILARLATGEATVSELAEPFAMSLP
                     AVTKHLKVLQRAGLVSQGRRAQWRPCRLEARPLQDAADWVGQYRRFWDERLDRLEDYL
                     RTLNALEPPHDDTP"
BASE COUNT          690 a         1753 c         1906 g          655 t
ORIGIN      
        1 ccgcacagcc cccgcccccg gtgctagcat ccaccccctc atgcagatcc acgacctcca
       61 ccagcgcctc gccgacctgg gcgccaagcc cgcccacatc cagcgcatcc tgcgcgcgtg
      121 gacccatgcc cagcctctgg acagcgggcc gacgcggcgc gggccggctt tgccgaaggc
      181 ggtgatggag gcgctgccgg cgctggaggc ggagctggcg gggttggcgc ggctgcgttc
      241 ggagcatccc ggcgaggatg gctcggcgcg gctgctggtg tcgctgccgg acgggcacac
      301 ggtggagagc gtgctgctgc tgcgggacgg gctgtgcgtg tcgagccagg tgggctgcgc
      361 ggtgggctgc gtgttctgca tgaccgggcg ggacgggctg ctgcgtcagg tgggcagcgc
      421 cgagatcgtg gcccaggtgg tgctggcgcg gcgcctgcgg ccggtgaaga aggtggtctt
      481 catgggcatg ggcgagccgg cccacaacct ggacaacgtg tgggacgcga tccagttgct
      541 gggcagcgcg ggcggcatcg cccacaagaa cctggtgttc tccacggtgg gcgaccggcg
      601 cgtcttcgag cgcctgcccg agggcccggt gaagccggcg ctggccctgt acctgcacac
      661 cacgcgggct gacctgcggg ccgagctgct gccccgcgcg ccgcgcatcg accccgccga
      721 gctggtggaa ctggccgacg cctacgcccg cgccacgggc tacccgatcc agttccagtg
      781 gaccctgctg gacggcatca acgacacgcc ggaggagctg gacggcctcg tgcgcctgct
      841 cgccgggcgc tacgcgatcc tcaacatgat cccctacaac agcgtgccgg acctgcccta
      901 tcgccgcccg gcgcaggccc gcgccgcgga gatcgcccgc agcctgcacc gccgcggcgt
      961 gctcaccaag ctgcgcacct cggcggggca ggacgtggac gggggctgcg gccagctgcg
     1021 ggcgcgcagc attgccggcg aaacgccggt gcgggtgctg cgccgtcggg ggccggacgg
     1081 cgcctgagcc gcgcgccggc gcgcttcagc cggtgggggt cggcgccggc cagcgcacct
     1141 cgacccgcag cccgcgcccg ccggcgccgc tgcccagagc caggcgggcg ccgtgcagat
     1201 cgacaatgcg cgccacgatg gcgaggccca ggccgctgcc gggggcgctg ccggcctcca
     1261 ggcggacgaa gcgggccagc acggactcgc gctgcccggg cgggatgccg gggccgtcgt
     1321 cctccacgcc gaggacgatg gcgccgccgc ccggcccctc ggctgcccgc gccacgccca
     1381 cccgcagacg gccgccttcc ggggtgtagc gcagggcgtt gtccaccaga ttgcgcagca
     1441 gcacgcgcag ccattccgcc tgcccgaaga cgacgcagcc gggctcggcg gccagttcga
     1501 attccagccc gcgggccagg atggcgctgc ccagctcggc gcacacgtcc tcggccaggg
     1561 cggccagatc caccgcccgc ggatcgggca ggccggattc cgggtcgagg cgggccagct
     1621 gcagcatctg ctcgaccagg tgggcggcgc gggcgaggcc gctctggatc tgcgccaggg
     1681 agcgcgcccg ctcggcctcg tcgcgggcgc gcagggccac ctggagctgg gtctgcaggg
     1741 cggcgagggg ggtgcgcagc tcgtgggcgg cgtcggcggt gaagcgccgc tcggcctcca
     1801 gggcgctgtc cacccggcgg aagaggccgt tgagggcctc cagcatgggg cggatctcct
     1861 ccggcgcgga ggccggcgag acggcctgga gctgctccgg ggcgcggctg gcaatctgcc
     1921 cggcgaccac gtcgagggag gccagcccgc gccgcgtggc cagccacacg ccgagaccca
     1981 gcaggggcag gccgaagagg gcgggcagga cgatgcgcca ggcgatgtgg gcgatcaggt
     2041 cgtcgcggat gcggtggtcc tcgctgacct gcacctgcag gcggcgggcg ctgttccact
     2101 ggctgaagtg gcgccagtgg ccggcgccgt cctcggtctc ggagaagccg tcgatgggcg
     2161 tgaggggcgc ggtgccggcg gtctcggagc gcatcgcgag ctgcccgtcg gcatcccaga
     2221 tctggaagcg caggcgccgc tggtacttgt gcacggcctc ctccatgcga ctcacggcgc
     2281 ggccgccgcc ctggtcggcg aggaccagca gggtctggcc ggcctgagcg agctgggcgt
     2341 cgaagagttc gtccacctcg tggtgggcgt ccacgtagct gaacaccatg gtcacccccc
     2401 aggccaccga cacgccgccc agcagcaggc cgagcaggcg ccggcgcagg gagaaccaca
     2461 aggtgttcat cgggggatgg tgtagccgac gccgcgcagg gtgcggatca gctcgctgcc
     2521 gagcttcttg cgcaggtggt ggatgtgcac ttcgagggcg ttgctgtccg gttcgtcgcc
     2581 ccagccgtag agggactgct ccagctggct gcgggtgagc acccggccgg cgccctcgag
     2641 gagcatctgc agcagggcga actcgcggcc ggacagttcc accggctcgc cggcgcgggt
     2701 caggcgccgc gccgccgggt cgaggaccag ttcgccccac accagcagcg gggcgctgcg
     2761 cccggcgctg cggcggatca gggcgcggat gcgggcggcc agctcgtcca ggtccaccgg
     2821 cttgaccagg tagtcgtcgg cgccgccgtc cagcccggcc accttgtcgc ccaccgcgtc
     2881 gcgggcggtg aggatcagca ccggcacggc ctggccgcgg gcgcgcacgc gggccagcac
     2941 gtccagcccg cccaggcgcg gcaggcccag gtccagcacc agcaggtcga aggcttcggc
     3001 ctgcagggcg acatccgcag cctggccgtc gcggacccag tccacggtga agccggcctg
     3061 acgcaggccg acggtgaggc cgtcgccgag ctggggatcg tcttccgcga ggaggatgcg
     3121 catgggcgga gtgtagcggg ccgcggcccc gacgttcagg cgatcccggc ggcgagggca
     3181 cgccactggg cggcgaggac cggatcgtcc agcagcggaa cgagctcggc cggcgccggc
     3241 caggacgggt tcgggaagtg gcgggcgtgg cgctcgccgg tcatgcacgc cgggcgcacc
     3301 agctgtccgg cgtgggcgag ggtgtgggcg gtggcgatgc cttcggcgat gagggcgtcg
     3361 atgaaggccg ggtcgcggtt catcttggag gcgtggtcca gcgtataggc cgccagcggg
     3421 cggccgctgt cgtcctcgtt ggcgaggggc aggaaggcca cggtgacggt cttgtagcgg
     3481 ctgaacagcg gcgccctgag ggccgcgtcg tcggccggca gctgcgcgcg cagggcgtcc
     3541 agctcggcgg cgatctggtt caccgtggcg atactgcgca gctcctggcc gagggacagg
     3601 ttgccggcca gctcgttgcg gcggtcgtcg atctgggacg gggtcatggg cgcccggcca
     3661 gagtaggagg acgggttgat ctggacgatc cagaccaggt ccggcttgtg ctcggcctcg
     3721 cggttgcaga agaagttggt gaggggcggg ttctgggaat acaggccgtc ccagaaatag
     3781 ctttcccgat ggcgtccgct gcggatcttc agtttctggg cgaagaacag ttcggggatg
     3841 caggcgctgg cgagcagctc atccacatgc agcgcatcgc ccgccgtggc gggaaaggtc
     3901 cggaaggccg agcccggcga cacggcgtcc cggttgatga catcgacggc gcccacgtac
     3961 agatccaccc cggcacgccc gcggatggcg tcggcctccg gcggcaggtc cagcgcggcg
     4021 cggatgtcgg tgcgcatgcg atcctgggtg agggtcgagg tcagcggatt gggcggccag
     4081 gagacggtgg ggccgaaatc ctcgaaattc gccagccagc gggcggacgc gttggtgaag
     4141 aattcgcccc accaccaggg atccttgtcc tggaactgca gggacgcctt gttgcggttc
     4201 cagaagcgca gcacgcgctg cgccccttct gcccagccgc ccgcgcccgg atcgctccag
     4261 gccatggccg ccgtgatggc gccgccggag gtgccggaca agccggtcag ctggaagggg
     4321 acgcgcccgc cgggctggcg ccggaaatgc tcgaaggcca gcaacaggta agacagcgcc
     4381 cccgcggtaa atgccgtatg agccccgccg ccctggcagg cgatggcgag gcggggaagg
     4441 ggctcggaca tgatggactc ctggaaacgg tctgcgccac ggcggggaca ccccccatcg
     4501 cgcgcaacag cggcagatgg tgccgcattg taatgcgtgc gtccttcagc ctcctgtcag
     4561 gcgccgcggc agacgccacc gattcttctt gcggattccg ccgccggcat ctaatttacc
     4621 aagtggttaa ttaaagatcc ggtcctcccc atgtccgccg acgccctcag cgccaccttt
     4681 gccgccctcg ccgacccgac ccgccgggcc atcctggcgc ggctggcgac gggcgaggcg
     4741 accgtctcgg agctggccga gcccttcgcc atgagcctgc cggccgtcac caagcacctc
     4801 aaggtgctgc agcgcgccgg cctggtcagc cagggccgcc gcgcccagtg gcggccctgc
     4861 cggctggagg cgcggcccct gcaggacgcc gcggactggg ttggccagta ccggcgcttc
     4921 tgggacgaac gcctggaccg cctcgaagac tacctgcgca ccctcaatgc cctggagccg
     4981 ccccatgacg acaccccctg atgc
//
LOCUS       DAOQMM010000152         4997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_10175_length_4997_cov_51.434844, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000152 DAOQMM010000000
VERSION     DAOQMM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10175_length_4997_cov_51.434844"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1770)
                     /locus_tag="PLH85_16275"
     CDS             complement(103..1770)
                     /locus_tag="PLH85_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB66175.1"
                     /translation="MKLRHLLPAALALILGASALAGDAIGAAQKQPLNLTAIGMFFVF
                     VVATMGITYWAASRTKSTSDFYTAGGGITGFQNGLAIAGDYMSAATLLGLSALTYAKG
                     LDGFIYAIGFFVGWPVILFLMAERLRNLGKFTFADIASYRLEQGTIRTFAAFGSLTVV
                     CFYLIVQMVGAGQLIKLLFGLDYTTAVIVVGVLMVIYVTFGGMIATTWVQIIKACLML
                     FGGTVLLFLALGQFGFSLEEAARKAVEAHKDGIKIMGPGSLMADPVTAVSLSLGLVFG
                     TAGLPHIMMRFFTVPNAKEARKSVFVASGCIGYFFMVVCALGLMAITIVGTNPEYFEG
                     GTVGGKLIGGGNMPVMHLAKAVGGNLFLGFMSAVAFATILAVVSGLALAGASAIAHDI
                     YARVIRKGTASETEEMRVSKLATIVLGVVAVLLGMMFEKQNIAFLVGLTFGIAASTNF
                     PILILSMYWKGLTTRGALLGGLVGLVSAVTFVVLSKAVWVVVLGNKEAIFPYEQPALF
                     SMPLAFFFTWLFSVTDGSARAKTDKEGYDDQYVRSQTGIGAAAASAH"
     gene            complement(1767..2078)
                     /locus_tag="PLH85_16280"
     CDS             complement(1767..2078)
                     /locus_tag="PLH85_16280"
                     /inference="COORDINATES: protein motif:HMM:NF016244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNB66176.1"
                     /translation="MSADIYRKVRANPKFAELEGKRSRFALLLTALVMVSYYALMMVI
                     AFAPEVLRTPITEGGMLTIGVPIGAVIIIGSWLLTGWFVSRSNGEFDALNNDIIREAS
                     K"
     gene            complement(2182..3825)
                     /locus_tag="PLH85_16285"
     CDS             complement(2182..3825)
                     /locus_tag="PLH85_16285"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNB66177.1"
                     /translation="MTREAMEYDVVIVGAGPSGLTTAIRLKQLAEQNGKELSVCVVEK
                     GSEVGAHILSGAVIEPRALEELFPDWKERGAPLNTPVVEDRFLFLTTEKAYKLPTPPQ
                     MHNHGNYIVSLGNVVRWLGQQAEELGVEIYPGFAASEVLYHDDGELRGSVKGIATGDM
                     GVEKNGEPGPNYQPGIELHARQTVFSEGCRGSLTKGLFEKFHLRDGADPQTYGIGIKE
                     LWEVRPEVHQPGLTVHTVGWPVGTDVYGGSFLYHLENNQVAVGFVVGLDYSNPHLSPY
                     EEFQRFKTHPEIRTFFEGGRRISYGARALNEGGFQSVPRLSFPGGVLVGDTAGFLNVP
                     KVKGTHMSMKSGIEAAEALFAHLTAEGQHGHECIGYPERLKQSWLWDELYTVRNIRPS
                     FRWGLWGGIAYSALDTFVLKGRAPWTLHHHADHTQLRKASECAPIVYPKPDGKISFDR
                     LSSVFISATNHTEDQPCHLRLKDASVPIATNLALYDAPETRYCPAGVYEIVTEDSGPR
                     MQINAQNCLHCKTCDIKDPTQNINWTVPEGGGGPNYPNM"
     gene            complement(3976..>4997)
                     /locus_tag="PLH85_16290"
     CDS             complement(3976..>4997)
                     /locus_tag="PLH85_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB66178.1"
                     /translation="GIHGSATAVMSFGDKGGAIGYLVGEANRGLEYMFTMMNHARLNV
                     GLEGVGVSERSYQHALAYARERIQGKIVGDKSGEKKPILHHPDVRRMLMDMKSRTEAG
                     RALAYYVAGCMDRAHSHPDADVRAANQRRLELLTPVVKGWCTENAQGITWNGVQVHGG
                     MGFIEETGAAQYMRDARIITIYEGTTAIQANDLIGRKTAKEGGKSMQQLLADIAETGA
                     ALRASGNDQLKTIAEALGDGIAALDEATQWLLANYDATPQAAHAGSVPFLKLTGIVVG
                     AWLLARSAAIAAQRLDQADAAYYTAKLATATYFAQHVVPEANSYRDAIVNGAAAVLAL
                     DEALF"
BASE COUNT          778 a         1421 c         1919 g          879 t
ORIGIN      
        1 aaaaaaagcg ggcgccctgg ggcgcccgaa acttcctcga gggggaggaa aagctgcgtt
       61 gcagcgggat aagggtcggt tgcgtggctc cgctgcggtc gctcagtggg cgctcgcggc
      121 ggcggcgccg atgccggtct gggagcgcac gtactggtcg tcgtagcctt ccttgtcggt
      181 cttggcgcgg gcgctgccgt cggtgacgga gaacagccag gtgaagaaga aggccagggg
      241 catggagaac agggcgggct gttcgtaggg gaagatggct tccttgttgc ccagcaccac
      301 cacccacacg gccttggaga ggaccacgaa ggtgaccgcg gagaccagcc cgaccagacc
      361 gcccagcagc gcgccgcggg tggtgaggcc cttccagtac atggagagga tcaggatcgg
      421 gaagttggtg gaggcggcga tgccgaaggt gaggcccacc aggaaggcga tgttctgctt
      481 ttcgaacatc atgcccagga gcacggccac cacgcccagg acgatggtcg ccagcttgga
      541 gacgcgcatc tcttcggttt ccgacgcggt gcccttgcgg atcacgcggg cgtagatgtc
      601 gtgggcgatg gcggatgcgc cggcgagggc gagaccggag accacggcga ggatggtggc
      661 gaaggccacg gcggacatga agccgaggaa gaggttgccg cccacggcct tggccaggtg
      721 catgaccggc atgttgccgc cgccgatgag cttgccgccg acggtgccgc cctcgaagta
      781 ctcggggttg gtgccgacga tggtgatggc catcaggccc agggcgcaca cgaccatgaa
      841 gaagtagccg atgcagccgc tggccacgaa gacgctcttg cgggcttcct tggcgttggg
      901 cacggtgaag aagcgcatca tgatgtgggg caggccggcg gtgccgaaga ccaggcccag
      961 ggacagggac acggcggtga ccgggtcggc catcagggag ccggggccca tgatcttgat
     1021 gccgtccttg tgggcttcca cggccttgcg ggcggcttct tccaggctga agccgaactg
     1081 gccgagggcc aggaagagca gcacggtgcc gccgaagagc atcaggcagg ccttgatgat
     1141 ctgcacccag gtggtggcga tcatgccgcc gaaggtgacg tagatgacca tcagcacgcc
     1201 caccacgatg accgcggtgg tgtagtcgag gccgaacagc agcttgatga gctggccggc
     1261 gccgaccatc tgcacgatca ggtagaagca caccacggtg agggaaccga aggcggcgaa
     1321 ggtgcggatg gtgccttgct caaggcggta ggaggcgatg tcggcgaagg tgaacttgcc
     1381 cagattgcgc aggcgttcgg ccatcaggaa gaggatcacc ggccagccga cgaagaagcc
     1441 gatggcgtag atgaagccgt ccagaccctt ggcgtaggtc agcgcggaga ggcccagcag
     1501 ggtggcggcg gacatgtagt cgccggcaat ggcgaggccg ttctggaagc cggtgatgcc
     1561 accgccggcg gtgtagaagt ccgaggtgga cttggtgcgg ctggcggccc agtaggtgat
     1621 gcccatggtg gccaccacga acacgaagaa catgccgatg gcggtcaggt tgaggggctg
     1681 tttctgggcg gcgccgatgg cgtcgcccgc cagggcgctg gcgccgagga tgagggccag
     1741 ggcggcgggg aggaggtgac gaagcttcac ttggaggcct cccggatgat gtcgttgttc
     1801 agcgcgtcga attcgccgtt ggagcggctg acgaaccagc cggtgagcag ccaggagccg
     1861 atgatgatga cggcgccgat gggcacgccg atggtgagca tgccgccttc ggtgatgggg
     1921 gtgcgcagca cctccggggc gaaggcgatg accatcatca gggcgtagta ggacaccatg
     1981 acgagggcgg tgaggaggag ggcgaaacgg cttcgcttgc cctccagttc ggcgaatttg
     2041 ggattcgccc ggactttccg gtagatgtcg gcggacatgt ggtctccttc acaaagggtg
     2101 gatggttgaa attttttatg gtttgggtgt tgcgggaggg ggtgctgttc tcctctcccg
     2161 gtcctgcggg tgctgctgtt attacatgtt cgggtagttg ggaccgccgc cgccttcggg
     2221 cacggtccag ttgatgttct gggtcgggtc cttgatgtcg caggtcttgc agtgcaggca
     2281 gttctgggcg ttgatctgca tgcgcggacc gctgtcttcg gtgacgatct cgtacacgcc
     2341 ggccgggcag tagcgggtct ccggcgcgtc gtagagggcc aggttggtgg cgatgggcac
     2401 cgacgcgtcc ttcaggcgca ggtggcaggg ctggtcctcg gtgtggttgg tggccgagat
     2461 gaagaccgag gacaggcggt cgaagctgat cttgccgtcg ggcttgggat agacgatggg
     2521 tgcgcactcc gacgccttcc tgagctgggt gtggtcggcg tgatggtgca gggtccacgg
     2581 tgcgcggccc ttgagcacga aggtgtcgag ggcgctgtag gcgatgccgc cccacaggcc
     2641 ccagcggaag ctcgggcgga tgttgcgcac ggtgtacagc tcgtcccaca gccagctctg
     2701 cttgaggcgc tccggatagc cgatgcactc gtggccgtgc tggccttcgg cggtgaggtg
     2761 ggcgaagagg gcttcggcgg cctcaatgcc ggatttcatg gacatgtggg tgcccttgac
     2821 cttgggcacg ttgaggaagc cggcggtgtc gcccaccagc acgccgccgg ggaagctcag
     2881 cctgggcacg gactggaagc cgccttcatt gagggcgcgg gcgccgtagc tgatgcgccg
     2941 gcccccttcg aaaaacgtgc ggatctccgg gtgggtcttg aagcgctgga attcctcgta
     3001 gggggacagg tgcgggttgc tgtaatccag acccaccacg aagcccacgg cgacctggtt
     3061 gttttccagg tggtagagga aggagccgcc atatacgtcg gtaccgacgg gccagcccac
     3121 ggtgtgcacg gtgaggccgg gctggtgcac ctcggggcgc acttcccata gttccttgat
     3181 gccgatgccg taggtctggg ggtcggcgcc gtcgcgcagg tggaacttct cgaacaagcc
     3241 cttggtgagg gagccgcggc agccctcgga gaagacggtc tggcgggcgt gcagttcgat
     3301 gcccggctgg tagttggggc cgggttcgcc gttcttctcg acgcccatgt caccggtggc
     3361 gatgcccttg accgagccgc gcagctcgcc gtcgtcgtgg tagagcactt cggaggcggc
     3421 gaagccgggg tagatctcga cgcccagttc ctccgcctgc tggccgagcc agcgcaccac
     3481 gttgcccagg ctcacgatgt agttgccgtg gttgtgcatc tggggcgggg tggggagctt
     3541 gtaggccttt tcggtggtca ggaacaggaa gcggtcctcg acgaccgggg tgttgagggg
     3601 cgcgccgcgt tccttccagt cgggaaacag ttcttccaga gcgcggggtt cgatgacggc
     3661 accggagagg atgtgggcgc cgacttcgga gcccttttcg acgacgcaca ccgagagctc
     3721 cttgccgttt tgctcggcca gttgcttcag gcggatcgcg gtggtcaggc cggaggggcc
     3781 ggcaccgacg atcaccacgt cgtattccat cgcttcgcgc gtcatctgct tctccgaaaa
     3841 tcgagcgtgt tcagttgaat ctgggctgca tccgggcacg cggtgcccgg gtgggggaaa
     3901 ggggccgccg gggatcgcct cccgcggggc cgccacaggg gcggcaccgc gggcggggcc
     3961 cggcggggga gacggtcaga acagggcttc gtccagggcc agcaccgcgg cggcgccgtt
     4021 cacgatggcg tcccggtagc tgttggcttc ggggaccacg tgctgggcga agtaggtggc
     4081 ggtggccagc ttggcggtgt agtaggcggc gtcggcctgg tccaggcgct gggcggcgat
     4141 ggcggcggag cgggccagca gccaggcgcc gacaacgatg ccggtgagct tgaggaaggg
     4201 caccgagccg gcgtgggcgg cctggggcgt ggcgtcgtag ttggcgagca gccattgggt
     4261 ggcttcgtcc agcgcggcga tgccgtcgcc cagggcttcg gcgatggtct tgagctggtc
     4321 gttgccggag gcccgcaggg cggcgccggt ctcggcgatg tcggcgagga gctgctgcat
     4381 gctcttgccg ccttccttgg cggtcttgcg gccgatcagg tcgttggcct ggatggcggt
     4441 ggtgccttcg tagatggtga tgatgcgggc gtcgcgcatg tactgggctg cgccggtctc
     4501 ttcgatgaag cccatgccgc cgtgcacctg cacgccgttc caggtgatgc cctgggcgtt
     4561 ctcggtgcac cagcccttga ccaccggggt gagcagttcg aggcggcgct ggttggcggc
     4621 gcgcacgtcg gcgtccgggt ggctgtgggc gcggtccatg cagccggcca cgtagtaggc
     4681 cagggcgcgg ccggcttcgg tgcgggactt catgtccatc agcatgcggc gcacgtccgg
     4741 gtggtgcagg atgggcttct tctcgccgga cttgtcgccg acgatcttgc cctggatgcg
     4801 ttcgcgggcg taggcgaggg cgtgctggta gctgcgctcg gagacgccga cgccttccag
     4861 gcccacgttg aggcgggcgt ggttcatcat cgtgaacatg tattcgaggc cgcggttcgc
     4921 ctcgcccacc aggtagccga tggcgccgcc cttgtcgccg aaggacatga cggcggtggc
     4981 gctgccgtgg atgccca
//
LOCUS       DAOQMM010000153         4973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_10239_length_4973_cov_36.304595, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000153 DAOQMM010000000
VERSION     DAOQMM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10239_length_4973_cov_36.304595"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PLH85_16295"
     CDS             <1..1022
                     /locus_tag="PLH85_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HNB66179.1"
                     /translation="AEQRATHQDRFLREDGIVMVATIAFGMGIDKPDVRFVAHLDLPR
                     SIEGYYQETGRAGRDGLPAEAWMTYSIADLVQQRRFIDQSEAGDDIKRVATAKLDALL
                     GLCETTGCRRQRLLGYFDEDSDPCGNCDTCLSPPQVRDATESARKALSCVFRTGSRFG
                     AGHVVDVLLGKTTDKVREWQHDQVSTFGIGTEFDDKGWRTLIRQLVAHGALAVDHERY
                     GALTLTEAARPLLRGETGFTLRLDTVAPKGRKSAPSARRAADWPEATGAEARLLDRLR
                     SWRAATAKARNVPAYVIFHDATLRDIAHQRPDSREALAAISGVGDRKLDAYADDLLGL
                     LEDAS"
     gene            1167..2351
                     /locus_tag="PLH85_16300"
     CDS             1167..2351
                     /locus_tag="PLH85_16300"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB66180.1"
                     /translation="MPPTEILIIDDDPAVRDMLSLYLRSDGHTVRTAASGPEGIARCH
                     EHPPSLVLCDLSMPGMSGLAVMSRLTADFPDLPVLVVSGTGDLGDAIEALKLGAWDFV
                     TKPIEDLAVLDHAIGRAFERARLLAENRAYRIHLENTNARLETTLRRLEEDEEGGRRI
                     QFALLPDARVDFRDITCTHFLVTSAILGGDFVDYFRIDDARFGFYMADVSGHGVPSAV
                     VTVLLKGFVSRFLENHRHYGDPTLLDPAALLAALNPLVLDGKHGKYLTMFYAVVDLDA
                     NTLSFANGGHFPLPLISDGGEVRELGGRSRPVGLFRDAQFNTETLTLPDRFALRLYSD
                     GVLELLDGPGNDARRATLRSVSRSLDLDARNLAARLGMESNRPLPDDASILSIRRHAP
                     HD"
     gene            2344..2853
                     /locus_tag="PLH85_16305"
     CDS             2344..2853
                     /locus_tag="PLH85_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB66181.1"
                     /translation="MIDDTHGRVLYARDGDTWVLRFTGAIRYTMAHAVDSFLDELFSR
                     QHPGRICVDLNDTESIDSTGIGLLAKLANGLARHGGERPVFFTSNPEILETLHNVCLD
                     DLCTLVAGAPTAVAANEIPATTPDERQLAGTIVAVHRLLCDMNAENRAKFQSVVDAFE
                     RDLAPEAHK"
     gene            2850..4529
                     /locus_tag="PLH85_16310"
     CDS             2850..4529
                     /locus_tag="PLH85_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB66182.1"
                     /translation="MSERDAEPAGPALSWAQELVEAAPDPIIVIDQGGAIVFANGQTE
                     RLFGYPRNELLGQPIEHLVPERFRHTHHAKRASFQAHPSFRPMGSGLDLTARCRDGRE
                     IPVEISLAPLHTADGVFISAAIRDITERRRTQKALRDARDQLEARVHARTAELEHANQ
                     ALQTEMADRLKAEGALRQAQKMEAVGQLTGGIAHDFNNLLTVIMGNLQMLLRQTRDNA
                     LASELAQAAFKAARRGSELNRTLLAFSRRQHLAPAAVDLNELVTHMGTMLQRTLGEQI
                     NIQLGLAPGLPRALADPGQLETAVLNLAVNARDAMPDGGTLSLDTASVVLDSHYTATE
                     VDITPGRYLRLTVSDSGHGMDAATVARACEPFFTTKEVGQGSGLGLAMVYGFVKQSGG
                     HIKIYSEPGLGTSIKLFLPEVQRDADTPTDAPDSPTDVEPRGGHETLLIVEDEEAVRN
                     MVCRVLESLGYRILVATQGNEALELLQTHPEIALLFTDVVLPGGLRGPDIARHARSLR
                     PDLPVLFTSGYTGTALPLQDSTDTPPFPLITKPYPIEELARMIRGALEGGR"
     gene            4631..4843
                     /locus_tag="PLH85_16315"
     CDS             4631..4843
                     /locus_tag="PLH85_16315"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNB66183.1"
                     /translation="MRACEVVFTLLAQAHHIDADLADGLRQMIGFRNIVLHDYMALQL
                     PIMVAIIEQNLEGVLKFAGEAGVKSQ"
BASE COUNT          855 a         2128 c         1310 g          680 t
ORIGIN      
        1 ccgccgagca gcgcgccacc caccaggacc gcttcctgcg cgaagacggc atcgtgatgg
       61 tcgccaccat cgccttcggc atgggcatcg acaagcccga cgtgcgcttc gtcgcccacc
      121 tcgacctgcc ccgctccatc gaaggctact accaggaaac cggccgcgcc ggccgcgacg
      181 gcctgcccgc cgaagcctgg atgacctaca gcatcgccga cctcgtccag cagcgccgct
      241 tcatcgacca gtccgaagcc ggcgacgaca tcaagcgcgt cgccaccgcc aagctcgacg
      301 ccctcctcgg cctctgcgag accaccggct gccgccgcca gcgcctcctc ggctacttcg
      361 acgaagactc cgacccctgc ggcaactgcg acacctgcct cagccccccg caagtgcgcg
      421 acgccaccga gtccgcccgc aaggccctct cctgcgtctt ccgcaccggc agccgcttcg
      481 gcgccggcca cgtggtggac gtcctcctcg gcaagaccac cgacaaggtg cgcgagtggc
      541 agcacgacca ggtcagcacc ttcggcatcg gcaccgagtt cgacgacaag ggctggcgca
      601 ccctcatccg ccagctcgtc gcccacggcg ccctcgccgt cgaccacgaa cgctacggcg
      661 ccctcaccct caccgaagcc gcccgcccgc tgctgcgcgg cgaaaccggc ttcaccctgc
      721 gcctcgacac cgtcgccccc aagggccgca agagcgcccc cagtgcccgc cgcgccgccg
      781 actggcccga agccaccggc gccgaagccc gcctcctcga ccgcctgcgc agctggcgcg
      841 ccgccaccgc caaggcccgc aacgtgcccg cctacgtcat cttccacgac gccaccctgc
      901 gcgacatcgc ccaccagcgc ccggacagcc gcgaagccct cgccgccatc tccggcgtcg
      961 gcgaccgcaa actcgacgcc tacgccgacg acctcctggg cctcctcgaa gacgcctcct
     1021 gacgcgccgc gcccaacggc gcgcaggggc tgcgtcgaaa ctttggccta tacggcaggg
     1081 tcagagcatg agagactttc gtgaccccaa cggaccgtct gacccacgga tccatcgacc
     1141 cctcaccaca aaaaagggga gacccgttgc ccccaacgga aatcctgatc atcgacgatg
     1201 atcccgccgt ccgggacatg ctcagcctgt acctgcgcag cgacgggcac accgtgcgca
     1261 ccgccgcgag cggccccgag ggcatcgccc gctgtcatga gcacccgccg tccctcgtcc
     1321 tgtgcgacct gagcatgccc ggcatgagcg gcctcgccgt catgtcccgg ctcaccgccg
     1381 atttccccga tctcccggtc ctcgtcgtct ccggcaccgg cgacctgggc gacgccatcg
     1441 aagcgctcaa gctcggcgcc tgggatttcg tcaccaagcc catcgaagac ctggccgtgc
     1501 tcgaccacgc catcggccgc gcctttgaac gggcccgcct gctcgccgag aaccgggcct
     1561 accgcatcca cctggaaaac accaacgccc gcctcgaaac caccctgcgg cgcctcgaag
     1621 aagacgagga aggcggccgc cgcatccagt tcgccctgct gcccgatgcc cgcgtggact
     1681 tccgcgacat cacctgcacc catttcctcg tcacctccgc catcctcggg ggtgacttcg
     1741 tcgattactt ccgcatcgac gacgcccgct tcggcttcta catggccgac gtctccggcc
     1801 acggcgtccc ctccgccgtc gtcaccgtgc tgctcaaggg cttcgtcagc cgcttcctcg
     1861 agaaccaccg ccactacggc gaccccaccc tgctcgaccc cgcggccctc ctggcggccc
     1921 tcaaccccct cgtcctcgac ggcaagcatg gcaagtacct caccatgttc tatgccgtcg
     1981 tcgacctcga cgccaacacc ctcagcttcg ccaacggcgg ccacttcccg ctgcccctca
     2041 tcagcgacgg cggcgaggtg cgcgagctcg gcggccgcag ccgtccggtc ggcctcttcc
     2101 gcgacgccca gttcaacacc gaaaccctca ccctgcccga ccggttcgcc ctccgcctct
     2161 actccgacgg cgtgctcgaa ctgctcgacg gccccggcaa cgacgcacgg cgcgccaccc
     2221 tgcgcagcgt ctcccggtcc ctcgacctcg acgcccgcaa cctcgcggcc cgtctgggca
     2281 tggaaagcaa ccgtcccctc ccggacgacg ccagcatcct cagcatcagg aggcacgccc
     2341 cccatgattg acgacactca cggccgcgtg ctctacgcgc gcgacggcga cacctgggtc
     2401 ctgcgcttca ccggcgccat ccgctacacc atggcccacg ccgtcgacag cttcctcgac
     2461 gagctcttca gccgtcagca ccccggccgc atctgcgtcg acctcaacga caccgaatcc
     2521 atcgacagca ccggcatcgg cctcctcgcc aagctcgcca atggcctcgc ccgccacggc
     2581 ggcgaacgtc cggtcttctt cacgtccaac cccgagatcc tagagaccct gcacaacgtc
     2641 tgcctggacg acctgtgcac cctcgtcgcc ggcgctccca ccgccgtcgc cgccaacgag
     2701 atccccgcca ccacgccgga cgaacggcag ctggccggca ccatcgtcgc cgtccatcgt
     2761 ctgctgtgcg acatgaacgc cgagaaccgc gccaagttcc agagcgtcgt ggacgccttc
     2821 gaacgggacc tcgccccgga agcccacaaa tgagcgaacg cgacgccgag cccgcgggcc
     2881 ccgccctgtc ctgggcccag gaactcgtcg aagccgcccc cgaccccatc atcgtcatcg
     2941 accagggcgg cgccatcgtc ttcgccaacg gtcagaccga gcggctcttc ggctatcccc
     3001 gcaatgaact cctcggccag cccatcgaac acctcgtccc cgaacgcttc cgccacaccc
     3061 accacgccaa gcgcgccagc ttccaggccc accccagctt ccgccccatg ggcagcgggc
     3121 tcgacctcac cgcgcgctgc cgcgacggtc gcgagatccc cgtcgagatc agcctcgccc
     3181 ccctgcacac cgccgacggc gtcttcatct ctgccgccat ccgcgacatc accgaacggc
     3241 gccgcaccca gaaagccctc cgcgacgccc gcgaccagct cgaagcccgc gtacacgccc
     3301 gcaccgccga actcgaacac gccaaccagg ccctgcagac cgagatggcc gaccggctca
     3361 aggccgaagg cgccctccgt caggcccaga agatggaagc cgtcggccag ctcaccggtg
     3421 gcatcgccca cgacttcaac aacctcctca ccgtcatcat gggcaacctg cagatgctcc
     3481 tgcgccagac ccgtgacaac gccctcgcct ccgaactcgc ccaggccgcc ttcaaggccg
     3541 cccgccgcgg cagcgagctc aaccgcaccc tgctcgcctt ctcccggcgc cagcacctcg
     3601 cccccgccgc cgtcgacctc aacgagctcg tcacccacat gggcaccatg ctccagcgca
     3661 ctctgggcga gcagatcaac atccagctcg gcctcgcccc cgggctgccc cgcgccctcg
     3721 ccgaccccgg ccagctcgag accgccgtgc tcaacctcgc cgtcaacgcc cgcgacgcca
     3781 tgcccgacgg cggcaccctc agcctcgaca ccgccagcgt cgtcctcgac agccactaca
     3841 ccgccaccga agtagacatc acccccggcc gctacctgcg cctcaccgtc tccgactccg
     3901 gccacggcat ggacgccgcc accgtcgccc gcgcctgcga acccttcttc accaccaagg
     3961 aagtcggcca gggcagcggc ctcggcctcg ccatggtgta tggcttcgtc aaacaatccg
     4021 gcggccacat caagatctac agcgagcccg gcctcggcac ctccatcaag ctcttcctgc
     4081 ccgaagtgca gcgcgacgcc gacaccccca ccgacgcccc cgacagcccg acggacgtcg
     4141 aaccccgcgg cggccacgag accctgctca tcgtcgaaga cgaagaagcc gtccgcaaca
     4201 tggtctgccg cgtcctcgaa tccctcggct accgcatcct cgtcgccacc cagggcaacg
     4261 aagccctcga actcctccag acccacccgg agatcgccct cctcttcacc gacgtcgtcc
     4321 tccccggtgg cctccgcggc cccgacatcg cccgccacgc ccgcagcctg cgccccgacc
     4381 tgcccgtcct cttcacctcc ggctacaccg gcaccgccct ccccctgcag gacagcaccg
     4441 acacgccccc cttcccgctg atcaccaagc cctatcccat cgaggaactg gcacggatga
     4501 tccggggggc gctggagggt gggcggtagg cctcacccgc agcgtcgcac gcgcttgcga
     4561 ggcatacgcc acacacctgg ccagcttcgc cgctgacgtc acccgccagg gcgccgccct
     4621 cctcaacgtc ctgcgcgcct gcgaagtcgt cttcaccctc ctcgcccaag cccatcacat
     4681 cgacgccgac ctcgcagatg gactcagaca gatgatcggc ttccggaata tcgtcttgca
     4741 tgactacatg gcgctgcaac tgccgatcat ggtggcgatc attgaacaga atttggaggg
     4801 agttttgaag tttgctggtg aggcaggggt gaagtcccaa tagcacggta ataaacggct
     4861 gctttgctcc gaatttcgga cacgctcgct acgcggcttg cggtggagtc ggagtaggtc
     4921 gggttagcgc agcgtaaccc gacattgcac gatcagtccc actggcaccc aaa
//
LOCUS       DAOQMM010000154         4839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_10690_length_4839_cov_30.160117, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000154 DAOQMM010000000
VERSION     DAOQMM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10690_length_4839_cov_30.160117"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /gene="nirJ"
                     /locus_tag="PLH85_16320"
     CDS             complement(<1..1012)
                     /gene="nirJ"
                     /locus_tag="PLH85_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HNB66184.1"
                     /translation="MFRISSYIREIAVPTPLGPRRQPPGPVVIWNLIRRCNLTCKHCY
                     SISADTDFAGELSTDEVYRTMDDLKAFRVPVLILSGGEPLLRPDIFDIAARAKGMGFY
                     VGLSSNGTLIDAANIDRIAAADFDYVGISLDGIRDTHDRFRRLDGAFERSLAGLRRCR
                     DLGVKVGVRFTLTADNAHDLPAVLQLVEDEGIDRFYFSHLNYAGRGNKHRADDARHVL
                     TRQAMELLFETAWDYEQRGLVKDFTTGNNDADGPFFLHWVQRRFPERAAHIEAKLMQW
                     GGNASGVNVANIDNLGNVHPDTMWWHHTLGNVRERAFGAIWTDLSDPLLAGLKQSPRP
                     VSG"
     gene            1270..3945
                     /locus_tag="PLH85_16325"
     CDS             1270..3945
                     /locus_tag="PLH85_16325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB66185.1"
                     /translation="MSTAVRVTGRQLVIAGATFVTAIIAIVSVTAAMLLHQQEIDGWR
                     QQLASLSLALAEQTAQTMSATDLVLDSIVAEVDALHLHDAADLRALTRSETFHHFLKE
                     RVAGLSRADVATIVGANGEVINFTRSFPPPPINLSDRDYFKVHLADARVGTYISVPVR
                     NKGNGKWIFYVTRRLNDAEGRFAGLVLVGISVDQFSDFFQRIGASLGEGASVSLSRDD
                     FALLTRWPARENLIGERNLTGSNYEVIHTLGKSDDVISLDSPRMPEDFAPQRRMAAVR
                     KVEHFPLILNITVSHDLVLSNWYPAVWAIGAIAGGSILALLLGTFTGLRILARREDDA
                     RRLRESEERYARAVRGTSDGLWDWDLVAERDYLSPRLKDMLGFGGDPAITRMDDVLSR
                     VHPQDEPTVRKALRAQLDQHVPLDIELRVLTRGGSYRWMALRGEAERTPAGKPVRLSG
                     TLSDITARKLAEEEVLEHRNHLEDLVDARTAALSVAKEAAEAASRAKTAFLANMSHEL
                     RTPMNAIICLTHLLRRGANEPGQREKLAKITQSADHLLEIINNVLDLSKIEAERVSLE
                     RTHFRVSDVLHQVEGLLDEKARDKCLRLTLQADPAIAELDLLGDPLRLKQVLLNLTGN
                     AIKFTECGSVTVEAHRCGHTDEDVVIGFDVQDTGVGMSDGVLQRIFEPFEQADGSTTR
                     RYGGTGLGLAISRHLVELMGGQITVTSTPGAGSCFSFTVHLARAPAGDGGPDVSVLTP
                     DEAEQLLKQRYRGIRLLLAEDDLINQEVAMELLHHLTGLAVDLANDGVEAVEMARLGH
                     YDMILMDMQMPHLDGLAATRRIRALPGVGRTPIIAMTANAFEGDRRDCLEAGMDDFLA
                     KPFNPGSLLQVLLRWLPRPVVAPPPPARRRDARDQ"
     gene            complement(3939..4445)
                     /locus_tag="PLH85_16330"
     CDS             complement(3939..4445)
                     /locus_tag="PLH85_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNB66186.1"
                     /translation="MNPPDDLDRRLVIATQAGLPLVPRPYDALAAQLGVSAEVVRARL
                     AAMLENGRIRRIGAVPNHYALGYTANGMSVWDVDDARIVELGEAVGRLPGVTHCYERP
                     RALPDWPYNLFAMVHGRDRATVLAQVETIARLLGAACRARDVLFSTAILKKTGLRIGR
                     GGGDGAAY"
     gene            complement(4442..4834)
                     /locus_tag="PLH85_16335"
     CDS             complement(4442..4834)
                     /locus_tag="PLH85_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB66187.1"
                     /translation="MCDAPYQAVAEQLGTTEAEVLARLRRLLDERVLTRFGPMFQVER
                     LGGRFVLAALAVPEARFDTVAAQVNALPEVAHNYRREHHLNMWFVLATETPDGIADAI
                     RRIEADTGLMVFAFPKRREFFVDMRLQA"
BASE COUNT          772 a         1749 c         1592 g          726 t
ORIGIN      
        1 gcccgctgac gggccgcggc gactgcttga ggccggccag cagggggtcg gagagatcgg
       61 tccagatcgc cccgaaggcg cgttcccgca cattgcccag ggtgtggtgc caccacatgg
      121 tgtccggatg gacgttgccc aggttgtcga tgttggccac gttcacaccg ctggcgttgc
      181 cgccccactg catcagcttg gcctcgatat gggcggcccg ctcgggaaag cggcgctgca
      241 cccagtgcag gaagaagggc ccgtcggcat cgttgttgcc ggtcgtgaag tccttgacga
      301 gaccgcgctg ctcgtagtcc caggcggtct cgaagagcag ctccatcgcc tgacgggtga
      361 ggacgtggcg ggcatcgtcg gcgcggtgct tgttgccgcg gccggcgtag ttgagatgcg
      421 aaaagtagaa gcggtcgatg ccttcgtcct ccaccagctg cagcacggcg ggcaggtcgt
      481 gggcgttgtc ggcggtgagg gtgaagcgca cgcccacctt cacgcccagg tcgcggcacc
      541 ggcgcaggcc cgccagggag cgctcgaagg cgccgtccag gcggcgaaag cggtcgtggg
      601 tgtcgcggat gccgtccagg ctgatgccga cgtagtcgaa gtccgcggcg gcgatgcggt
      661 cgatgttggc ggcgtcgatc agcgtgccgt tggacgacag gcccacgtag aaacccatgc
      721 ccttggcgcg ggccgcgatg tcgaagatgt ccggtcgcag caggggctcg ccgccggaca
      781 ggatcagcac cggcacacga aaggccttca ggtcgtccat cgtgcgatag acttcgtcgg
      841 tgctcagttc gccggcgaaa tccgtgtcag ccgaaatgga gtagcaatgc ttgcaggtga
      901 ggttgcagcg ccggatcagg ttccagatca ccaccgggcc gggcggctgg cggcgcggtc
      961 cgaggggcgt gggcacggca atttcgcgga tgtagctgga gatgcggaac atggccggat
     1021 ttcctgaaga gggtgtccgg attgtcggcg ccctgcccgc ctgcgggcat tgacgcccat
     1081 caatcccgcg gtctcaagtc cggtccggaa tgggccgtta tattgcaagg ctgccgggca
     1141 atcccggagc cccggtgcac cgacggcgtc gtcgcgtgtc aatcccaccg cccgtctcga
     1201 tcccgccatg cagccgactc ccctcgccgt gttctgcagc gcccaaatcc ggagccggcc
     1261 gtgaccggga tgtccaccgc cgtccgggtc accggccggc agctcgtcat cgccggtgcc
     1321 accttcgtca ccgcgatcat cgccatcgtc tcggtgacgg cagcgatgct cctgcaccag
     1381 caggagatcg acggctggcg ccagcaactc gccagcctca gcctggccct ggccgagcag
     1441 accgcccaga ccatgtccgc caccgatctg gtgctggaca gcatcgtcgc cgaggtcgat
     1501 gccctgcacc tgcacgacgc cgccgacctg cgcgccctga cccgctccga gaccttccac
     1561 cacttcctca aggagcgcgt ggccgggctg tcccgggccg acgtggcgac catcgtcggc
     1621 gccaacgggg aagtcatcaa cttcacccgc agctttccgc caccgcccat caacctgtcc
     1681 gaccgggact acttcaaggt tcatctggcc gatgcccgcg tgggcaccta catcagcgtg
     1741 ccggtgcgca acaagggcaa cggcaagtgg atcttctacg tcacccgccg cctcaacgac
     1801 gccgaggggc gctttgccgg gctggtcctg gtgggcattt cggtggacca gttctccgac
     1861 ttcttccagc gcatcggcgc ctccctcggc gagggggcct ccgtgtccct gtcccgcgat
     1921 gacttcgccc tgctcacccg ctggcccgcc cgggagaacc tgatcggtga gcgcaacctc
     1981 acgggcagca actatgaggt gatccacact ctgggcaaat ccgacgacgt catctccctc
     2041 gactcgcccc gcatgcccga ggatttcgcc ccccagcgac gcatggccgc cgtgcgcaag
     2101 gtggagcact tcccgctgat attgaacatc accgtcagcc acgacctggt cctgagcaac
     2161 tggtacccgg ccgtatgggc catcggtgcc atcgccggtg gcagcatcct cgccctgctg
     2221 ctgggcacgt tcaccgggct gcgcatcctc gcccgccgcg aggacgatgc ccgacgcctg
     2281 cgcgagagcg aggagcgcta cgcccgcgcc gtgcgcggca ccagcgacgg cctgtgggac
     2341 tgggacctgg tggcggagcg ggactacctg tcaccgcgcc tcaaggacat gctgggcttc
     2401 ggcggcgatc ccgccatcac ccgcatggac gacgtcctga gccgcgtcca tccccaggac
     2461 gaacccaccg tgcgcaaggc cttgcgggcc cagctggacc agcacgtgcc cctcgacatc
     2521 gaactgcgcg tgctcacccg cggcggcagc taccgctgga tggccctgcg cggcgaggcc
     2581 gagcgcacac ccgccggcaa gcccgtgcgc ctgtccggca ccctcagcga catcaccgcc
     2641 cgcaagctgg ccgaagagga agtcctcgag caccgcaatc acctcgagga tctggtggat
     2701 gcccgcaccg ccgcgctgtc cgtcgccaag gaggccgccg aggccgccag ccgcgccaag
     2761 accgcgttcc tcgccaacat gagccacgag ctgcgtacgc cgatgaacgc cattatctgc
     2821 ctcacccacc tgctgcggcg cggcgcaaat gagcccggcc agcgggaaaa gctcgccaag
     2881 atcacccagt ccgccgatca cctgctggag atcatcaaca acgtcctcga tctgtccaag
     2941 atcgaggccg agcgcgtgag cctggaacgc acccacttcc gcgtcagtga cgtgctgcac
     3001 caggtggaag gcctgctcga cgaaaaggcc cgtgacaagt gcctgcgcct caccctgcag
     3061 gccgacccgg ccatcgccga actggacctg ctgggcgatc ccctgcgcct gaagcaggtg
     3121 ctcctcaacc tcaccggcaa cgccatcaag ttcaccgaat gcggcagcgt caccgtcgag
     3181 gcgcaccgct gcggccacac cgacgaggac gtggtgatcg gcttcgacgt gcaggacacc
     3241 ggcgtgggca tgtcggacgg cgtcctccag cgcatcttcg agcccttcga gcaggccgac
     3301 ggctccacca cccggcgcta cggcggcacc ggcctggggc tggcgatctc ccgccatctg
     3361 gtggagctca tgggcggaca gatcaccgtg accagcacgc cgggcgcggg cagctgcttc
     3421 agcttcacgg tccatctggc gcgggcgccg gccggcgacg gcgggcccga cgtgtccgtc
     3481 ctgacccccg acgaagccga gcagctcctc aagcagcgct accggggcat ccgcctgctg
     3541 ctggccgagg acgacctgat caaccaggaa gtggccatgg agctgctcca tcacctcacc
     3601 ggcctcgccg tggatctggc caacgatggc gtcgaggcgg tggagatggc ccgcctcggc
     3661 cactacgaca tgatcctgat ggacatgcag atgccccacc tggatggtct ggcggccacc
     3721 cgtcgcatcc gcgccctgcc cggggtcggt cgcacaccca tcatcgccat gaccgccaac
     3781 gccttcgaag gggatcgccg cgactgcctg gaagccggca tggacgactt cctcgccaag
     3841 ccgttcaatc cgggcagcct gcttcaggtt ctgctgcgct ggctgccccg gccggtggtc
     3901 gcgccgccac cccccgcgcg tcgccgcgac gcccgcgatc agtaggccgc gccgtccccg
     3961 ccgccgcggc cgatgcgcag cccggtcttc ttcaggatcg ccgtcgagaa cagcacgtca
     4021 cgggcccggc aggccgcccc cagcaagcgt gcgatggtct ccacctgtgc cagcaccgtg
     4081 gcccggtcgc ggccatgcac catcgcgaac aggttgtagg gccagtccgg cagggcgcgc
     4141 gggcgctcgt agcagtgggt gaccccgggc agacggccca ccgcttcgcc cagctcgacg
     4201 atgcgggcgt cgtccacgtc ccacacggac atgccgttgg ccgtgtagcc cagagcgtag
     4261 tggttgggca ccgcaccgat gcggcggatg cggccgttct cgagcatcgc cgccaggcgc
     4321 gcccggacga cctcggcgct cacccccagc tgtgccgcca gggcgtcgta gggccgcggc
     4381 accaggggca ggccggcctg ggtcgcgatc accaggcggc ggtccagatc atccggcggg
     4441 ttcacgcctg caacctcatg tccacaaaga actcccgccg cttgggaaag gcgaatacca
     4501 tgaggccggt gtcggcctcg atgcgccgga tcgcgtcggc gatgccgtcc ggcgtctcgg
     4561 tggcgagcac gaaccacatg ttgaggtggt gctcgcgccg gtagttgtgg gccacctcgg
     4621 gcaaagcgtt gacctgggcc gccacggtgt cgaagcgcgc ttccggcacc gccagcgctg
     4681 ccagcacgaa gcgtccgccc aggcgttcca cctggaacat gggcccgaag cgggtcagga
     4741 cccgctcgtc cagcagacga cgcaggcgcg ccagcacctc ggcctcggtg gtgccgagct
     4801 gctccgccac cgcctggtag ggcgcgtcac acaggggga
//
LOCUS       DAOQMM010000155         4766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_10962_length_4766_cov_47.221821, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000155 DAOQMM010000000
VERSION     DAOQMM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10962_length_4766_cov_47.221821"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..834
                     /gene="yaaA"
                     /locus_tag="PLH85_16340"
     CDS             49..834
                     /gene="yaaA"
                     /locus_tag="PLH85_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNB66188.1"
                     /translation="MLFVISPAKALDFDTPPTTPRFTEPDFLDDAAELIAGLRTLSHA
                     QVAELMDLSDALAALNVARYEAWARPFSPQNAKPALLAFDGDVYDGLGAASLREAELD
                     YAQAHLRILSGLYGVLKPLDLMQPYRLEMGTRYANGRGKDLYAFWGDRITEALNACLA
                     AEAVAGRAPVLVNLASEEYFKSVRPKKLKGRLLNVAFEDWKNGRYRIISFYAKRARGL
                     MARHAIRLGAQDVEQLKTFDAAGYAFAPEASDDGKWVFRRRVA"
     gene            complement(875..1243)
                     /locus_tag="PLH85_16345"
     CDS             complement(875..1243)
                     /locus_tag="PLH85_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66189.1"
                     /translation="MDFDELHERACINLRLLLPQVQDPTRLVDECLLSARKSFRDDFI
                     LSLSPEAWARISLVYISTFLDSGVDVPVGDIVAAVVSDSIHLRRIDLLAQQVGQRRAE
                     DKTARPSNGANGACLKVVKS"
     gene            complement(1365..2237)
                     /gene="asd"
                     /locus_tag="PLH85_16350"
     CDS             complement(1365..2237)
                     /gene="asd"
                     /locus_tag="PLH85_16350"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNB66190.1"
                     /translation="MKTRSLIDRLTNQEDLNFLLTNRVPRIALTRFMGWFSKIEHPLV
                     CRLSIAIWKLFADLDLSEARHTDFRSLHDCFTRQLKDGARPVDPDPAVLTSPCDAIVG
                     ACGPIAGTELIQAKGFPYTLLDLLADPALVDTYRDGSYVTLRLKSSMYHRFHAPADCR
                     VEHTTYISGDVWNVNPVALKRVEKLYCKNERAVIRTRLAADGAPITLVPVAAILVASM
                     RLHWLDVLLHLRYRGPNEMPVRADFRKGQEMGWFEHGSTIIVFAPKGFTLVDGIASGH
                     AIRMGHALLRRPGH"
     gene            2295..3950
                     /locus_tag="PLH85_16355"
     CDS             2295..3950
                     /locus_tag="PLH85_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB66191.1"
                     /translation="MEDALVWAGLGALALPGLALKIKRRLELSKGKHPSLAGHAKMSR
                     RVARLLPFYEYDEAGFFNSDDAPAEVVARRREGFFRLAELYRARFARSAALTAEAAEG
                     VSDLQFTGAYRVPFQYSRLVREHLKGGSFYTASAGVTVTDADGNVFYDLAGSYGVNVF
                     GYDFYKTCMARGAERVAALGPVLGSYHPVLLENVRRLKAVSGLDEVSFHMSGTEAVMQ
                     AVRLARYHTRRNHLVRFCGSYHGWWGDVQPGVGNPVAAEHTYTLRDMHPDALRVLRRR
                     KDIACVLINPLQGLHPNAAAPGDSSLVDSSRRAGFDRAAYTAWLKELRQVCTERGIVL
                     IFDEVFLGFRLAAGGAQEYFGVQADMVTYGKTLGGGLPVGVVCGRRDLMKRFRDDAPA
                     DICFARGTFNSHPHVMGAMAEFLARLETPEVKAVYAGLDDTWNGRAARLNARLLEAGL
                     PVQVANMQSVWTVFYTRPSRYNWMLQYYLRAEGLALSWVGSGRFIFSLNYSDADFEAV
                     AERFVAAAKQMEADGWWWHDGRTTNKDIRRRILKEMLRKRFLG"
     gene            complement(4095..4691)
                     /locus_tag="PLH85_16360"
     CDS             complement(4095..4691)
                     /locus_tag="PLH85_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66192.1"
                     /translation="MKNFLRALEAQRWDDHRFYHHSRINQTLHFISAISFVVAYCLIF
                     TDPVKAALIGWLVSMVTRQSGHFFFEPLGYDEENQCTNEYKEAVKVGYNLQRKVVLLS
                     LWAATPVVLYMDPTCFGLFDAHVDMDGYIDHLGKLWLSLGVAGLLFRTVHLFFLKDVQ
                     TGLVWLTKILTDPYHDIKLYHRAPLELLRELRARRQTA"
BASE COUNT          743 a         1407 c         1792 g          824 t
ORIGIN      
        1 tcctcccgcg tccctgtcac acacctcaca cgccctgtcg cacccgccat gctcttcgtc
       61 atctcccccg ccaaagccct cgacttcgac acgccgccca ccacgccgcg cttcacggag
      121 ccggattttc tggatgatgc ggcggagctg attgcggggt tgcggacttt gtcccatgcc
      181 caggtggcgg agctgatgga tctgtcggat gcgttggcgg ccttgaatgt ggcgcgttat
      241 gaggcgtggg cgcggccgtt ttcgccgcag aacgcgaagc cggccttgct ggccttcgac
      301 ggggatgtgt atgacgggct tggggcggcc agcctgaggg aggcggagct ggactatgcc
      361 caggcgcatc tgcgcatcct gtccgggctg tacggggtgt tgaagccgct ggatctgatg
      421 cagccttatc ggctggagat ggggacgcgc tacgccaatg ggcggggcaa ggatctgtat
      481 gccttctggg gcgaccgcat cacggaggcc ttgaatgcct gtctggcggc ggaggcggtg
      541 gccgggcggg cgccggtgct ggtgaatctg gcgtcggagg agtatttcaa gtcggtgcgg
      601 ccgaagaagc tgaaggggcg cttgctcaat gtggcctttg aggactggaa gaacgggcgt
      661 taccgcatca tcagcttcta tgccaagcgg gcccgcggcc tgatggcgcg gcacgcgatc
      721 cgcctggggg cgcaggatgt ggagcagttg aagaccttcg atgcggcggg ttatgccttt
      781 gcgccggagg cgtcggacga cgggaagtgg gtgttccgtc gtcgggtggc gtagcgggcg
      841 cccggggcgc ggacgtctgc gggcgctgcc ttcctcagga tttgaccacc ttcagacatg
      901 cgccgttggc gccattggat gggcgggcgg tcttgtcttc ggcgcggcgc tgaccgactt
      961 gctgggccag caggtcgatg cgtcgcaggt ggatggagtc gctgacgacc gcggcgacga
     1021 tgtcgcccac cggcacgtcc acgccggaat ccagaaaggt ggagatatac accagggaga
     1081 tccgcgccca ggcttccggg ctgaggctga ggatgaagtc gtcgcggaag gatttgcgcg
     1141 cgctgagcag acattcgtcc acgaggcggg tgggatcctg cacctggggc aacaacaggc
     1201 gcaggttgat gcaggcacgt tcgtgcagtt cgtcgaagtc catggcgcct ccggggacgg
     1261 ttgccatccg gacccgggcg tggccgggcc ttccacagtc gaacgataat tgcccgcgcc
     1321 gttgtcgtcc agagggcgcg ggtctgcggg gcctgcgcca gggttcagtg gcccggccgg
     1381 cgcagcaggg cgtggcccat gcggatggcg tggccggagg cgatgccgtc caccagcgtg
     1441 aagcccttgg gggcgaacac gatgatggtg gagccgtgct cgaaccagcc catctcctgg
     1501 cccttgcgga agtcggcgcg gacgggcatc tcgttggggc cgcggtagcg caggtggagg
     1561 agcacatcca gccagtgcag gcgcatgctg gccaccagga tggcggccac cggcaccagg
     1621 gtgatgggcg cgccgtcggc agcgaggcgg gtgcggatga cggcccgctc gtttttgcag
     1681 tagagctttt cgacgcgctt gagggcgacg ggattgacgt tccacacgtc gccggagatg
     1741 taggtggtgt gctccacgcg gcagtcggcg ggggcgtgga agcggtggta catgctggat
     1801 ttgaggcgca gggtgacgta gctgccgtcc cggtaggtgt ccaccagggc gggatcggcg
     1861 aggagatcca ggagcgtgta ggggaagccc ttggcctgga tcagctcggt gccggcgatg
     1921 gggccgcagg cgccgacgat ggcgtcgcag gggctggtga gcaccgcggg gtcgggatcg
     1981 acggggcggg cgccgtcctt gagctggcgg gtgaagcagt cgtgcaggct gcggaagtcc
     2041 gtgtgacggg cttcggacag gtccaggtcg gcgaagagct tccagatggc gatggagagg
     2101 cggcacacga gcgggtgctc gatcttgctg aaccagccca tgaagcgggt cagggcgatg
     2161 cgcgggacgc ggttggtgag gaggaagttg aggtcttcct ggttcgtgag ccggtcgatg
     2221 agggagcggg ttttcatggg gctgtaacgg ggttcggtta aatcagtcat ctttcagtcg
     2281 ccgaaaggaa atccgtggaa gatgcattgg tgtgggccgg gctgggggcg ctggccttgc
     2341 cgggtctggc gctcaagatc aagcgccgcc tggagctgtc gaagggcaag catccgtccc
     2401 tggccggaca cgcgaagatg tcccgccggg tggcgcgtct gctgccgttc tacgagtacg
     2461 acgaagcggg cttcttcaat tccgacgatg ccccggccga ggtggtggcg cgccgtcggg
     2521 agggcttctt ccgtctggct gagctgtacc gcgcgcgctt cgcccgcagc gcggcgctca
     2581 ccgccgaggc ggcggagggc gtgtcggacc tgcaattcac cggcgcctac cgggtgccgt
     2641 tccagtacag ccgcctggtg cgggaacacc tgaagggcgg cagcttctac accgcctcgg
     2701 cgggcgtgac ggtgacggac gcggacggca acgtcttcta cgacctggcc ggcagctacg
     2761 gggtgaatgt cttcggctac gacttctaca agacctgcat ggcccgcggt gccgaacggg
     2821 tggccgcgct ggggccggtg ctgggctctt accatccggt gctgctggag aatgtgcgcc
     2881 gcctgaaggc cgtttccggg ctggacgagg tgtccttcca catgtccggc accgaggcgg
     2941 tgatgcaggc cgtgcgcctg gcccgctacc acacccggcg caatcatctg gtgcgcttct
     3001 gcggcagcta ccacggctgg tggggcgacg tgcagccggg cgtgggcaat cccgtcgcgg
     3061 ccgaacacac ctacaccctg cgcgacatgc atccggacgc cctgcgcgtg ctgcgccggc
     3121 gcaaggacat cgcctgcgtg ctgatcaatc cgctgcaggg actccatccc aacgctgcgg
     3181 cgccggggga ttcgtcgctg gtggacagct cgcgccgcgc cggcttcgac cgggcggcct
     3241 acacggcgtg gctcaaggaa ctgcgccagg tgtgcaccga gcggggcatc gtgctgatct
     3301 tcgacgaggt cttcctgggc ttccgcctgg cggccggcgg cgcgcaggaa tacttcggcg
     3361 tgcaggcgga catggtcacc tacggcaaga ccctgggcgg cggcctgccg gtgggcgtgg
     3421 tgtgcggccg gcgcgatctg atgaagcgct tccgcgacga cgcgccggcg gacatctgct
     3481 tcgcccgcgg caccttcaat tcccaccccc acgtgatggg ggcgatggcg gaattcctgg
     3541 cgcgcctgga gacgcccgag gtgaaggcgg tctacgccgg cctggacgac acctggaacg
     3601 gccgcgccgc gcgcctcaac gcccgtctgc tggaggccgg cctgccagtg caggtggcga
     3661 acatgcagtc ggtgtggacg gtgttctaca cccgcccgag ccgctacaac tggatgctcc
     3721 agtactacct gcgggccgag ggcctggccc tgtcgtgggt gggcagcggg cgcttcatct
     3781 tcagcctgaa ctactccgac gcggacttcg aggccgtggc cgaacgcttc gtggcggcgg
     3841 cgaagcagat ggaggcggac ggctggtggt ggcacgatgg ccggacgacc aacaaggaca
     3901 tccgccggcg catcctcaag gagatgctgc gcaagcgctt cctgggctga gcggtccacg
     3961 cggcaaggcg gaacggcacg gacgtgcgtt ccgccttttt tgtgggccgg gcagtcgatg
     4021 gcgggcatgg ggcgctggaa acgacaccgc gccacccggc aaacccggcg tggcgcggtg
     4081 aggcgacgaa gggatcaggc ggtctggcgg cgggcgcgga gttcgcgcag cagctccagc
     4141 ggggcccggt ggtagagctt gatgtcgtgg taggggtcgg tgaggatctt ggtcagccac
     4201 accagcccgg tctgcacgtc cttcaggaag aacaggtgca ccgtgcggaa gagcaggccg
     4261 gccacgccca ggctcagcca cagcttgccc aggtggtcga tgtagccgtc catgtctaca
     4321 tgggcgtcga agaggccgaa gcaggtcggg tccatgtaca gcacgaccgg cgtggcggcc
     4381 cacaggctca gcagcacgac cttgcgctgc aggttgtagc cgaccttgac ggcttccttg
     4441 tattcgttgg tgcactggtt ctcttcgtcg tagcccaggg gctcgaagaa gaagtggccg
     4501 gactggcgcg tgaccatgga caccagccag ccgatcaggg cggccttgac cgggtcggtg
     4561 aagatcaggc agtaggccac gacgaagctg atcgcgctga tgaagtgcag cgtctggttg
     4621 atgcggctgt gatggtagaa gcggtggtcg tcccaacgct gtgcctcaag ggcacgcaga
     4681 aaatttttca tggagtctcc cggggttgtg aaagtgaaaa gctttcaagg cgagactaag
     4741 aaagttgtgt gactccgtgg ctgcgg
//
LOCUS       DAOQMM010000156         4641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_11422_length_4641_cov_48.922154, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000156 DAOQMM010000000
VERSION     DAOQMM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11422_length_4641_cov_48.922154"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..1187)
                     /locus_tag="PLH85_16365"
     CDS             complement(255..1187)
                     /locus_tag="PLH85_16365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB66193.1"
                     /translation="MDRLEELRVLLEIADGGSLAAGGRRTGRSPAAVSRILAGMERRL
                     GARLVERSTRSNVLTEAGRRLVEHARTLLADYDDALGDVSREASEATGRLRVSAPLVF
                     GRLHLAPLIGDFLVAHPGIHVDLVLSNALVDLREARIDVALRIDHLADSTLVAKRVGE
                     IRQRVVGSPAYLARVGTPCSPDDLAGHHAILHAHEDGVREWAFRLADGRVQRMRPPCR
                     WTVNQSETLIDAAIAGRGLIRVPSYQTVDHIEAGRLRCVLKGYEMPPVPVHLVFASTR
                     FIPLRLRRFLDFTAAALGTSTAFREPGDHTPNTD"
     gene            1317..2105
                     /locus_tag="PLH85_16370"
     CDS             1317..2105
                     /locus_tag="PLH85_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNB66194.1"
                     /translation="MSLSRRQFVQAALAGPALALDLPARAEARRVAPIQAVALDGFTT
                     FDPRPVAALAERLFPGQGAALTTAWRTRQFEYSWLRNSMDRYVDFWQVTEEALVFAAR
                     QTRLELDGTRRRQLMQAFLALDAWPDARPALERLHASGIRLAFLSNFTVEMLDAASRN
                     AGLAGLLEPHLSTDRVGVFKPHPRAYRMGVEAFGLPREAIAFAAFGGWDAAGAKAFGY
                     PTFWVNRLGLPVEQLGVQPDGIGASLEDLVRFVSTSNAASRPAA"
     gene            2129..2659
                     /locus_tag="PLH85_16375"
     CDS             2129..2659
                     /locus_tag="PLH85_16375"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNB66195.1"
                     /translation="MKSVLVSALCASLSVLSAPVSAQVQVSGRSVPLAIAVDAAAAAL
                     KRCEADGYRVTVSVVDAAGVERVRLRGDHSTIHTSETAFRKAYTVITLGPIFKATTTS
                     ALTERIGKTPNLPALSTIDHVIFLAGGVALQAQGEFLGAIGVGGAPGGALDEQCAQAG
                     VQAVQARLDGLGVPSN"
     gene            complement(2920..3831)
                     /locus_tag="PLH85_16380"
     CDS             complement(2920..3831)
                     /locus_tag="PLH85_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB66196.1"
                     /translation="MNSDTPFLAARQTPAHPAAVRLAHHEVNGTRIFYRSAGDPAQPA
                     IVLLHGFPASSHMFRDLIPHLAGHFHVIAPDYPGFGHSDAPATGRFPYSFDALAGVVD
                     GLLAHLGVDRYHLYMQDYGGPVGLRLATAHPERVLGLVIQNANAYLEGISPMVRDVFM
                     PLWEQGDEGPARALLDAATTRFQYLTGAGDPLAVSPDAWVFDQALLDRPGNAEIQLAL
                     FRDYRHNVDLYPVWHAYFRQRQPKTLIAWGRGDPLFLVEGAEAFRGDLPAAAVHYLDG
                     GHFVLEEHAARVAELILRHFTPERAAG"
     gene            3987..4616
                     /locus_tag="PLH85_16385"
     CDS             3987..4616
                     /locus_tag="PLH85_16385"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNB66197.1"
                     /translation="MSPAPTPFIFVADDVALDFANTLAAPTGTLFDFLGAPGSLTAWA
                     QASGALPALGALLVAAAAHGELAEVEDEARALRDWLVGVLPQIRAGSAPLAAVDLAPL
                     NRALTAACCAWSVHACEGRATLARDWCYAGPLSALAPLAEAIASLLARPDLHDIRKCE
                     NPQCTLWFRDVTKRGHRRWCSTAVCGNRAKVAAFRDRQKRATRETDGDG"
BASE COUNT          692 a         1524 c         1666 g          759 t
ORIGIN      
        1 ccggagaaag ccgggaaagc caaggaaatc aaggcttccc ggcttttttt cgtccagagt
       61 agtccgaaat gggcacttgc aatccagggg cattgacggg caaggccgcg cggaattgcc
      121 cccgaatgcc ccggaccgct gccgcgctcc gcgctgcccg gcccggcgag cagtctctca
      181 cgctgcctga cgcgcggtcc gggttcccgt gggcgccggc gcgttgcatc ggctccttgc
      241 acccgtgatc cgggctaatc ggtgttcggc gtgtggtcgc ccggttcgcg aaacgcggtg
      301 ctggtgccca gggcggcggc cgtgaaatcg agaaaccggc gcagacgcaa agggatgaat
      361 cgtgtgctgg cgaagaccag atgcacgggt acgggcggca tttcataccc tttgaggacg
      421 cagcgcagac gccccgcctc gatgtggtca accgtctgat aggacggcac gcggatcagg
      481 ccacgaccgg cgatggccgc gtcaatcagg gtttccgact ggttcaccgt ccaccggcac
      541 ggtggacgca tgcgctggac acgtccgtct gccagacgga aggcccactc ccgcacgccg
      601 tcctcgtggg cgtgcagaat ggcgtgatgg cccgcgagat cgtccggcga gcagggggtg
      661 ccgacgcggg cgaggtaggc ggggctgccc accacgcgct ggcggatctc gccgacgcgc
      721 ttggcgacaa gggtggagtc ggccaggtgg tcgatgcgca gggcgacgtc gatgcgcgcc
      781 tcgcgcagat ccacgagggc attgctgagc accagatcga cgtgtatgcc gggatgggcg
      841 acgaggaagt cgccgatgag gggtgccagg tgcaggcgcc cgaacaccag cggggcgctg
      901 acgcgcaggc ggccggtggc ttcgctggcc tcgcgggaga cgtcacccag cgcatcgtcg
      961 taatccgcca gcagcgtgcg ggcgtgttcc accaggcggc ggccggcttc ggtgagcacg
     1021 ttgctgcgcg tggagcgttc gacgagccgg gctcccaggc gtcgttccat acccgccagg
     1081 atgcggctca ccgcggccgg cgagcggccg gtgcgccgtc ctccggcagc gaggctgccg
     1141 ccgtcggcaa tctccagcag tacgcgcagt tcttcgagtc ggtccatggt tccttccgcc
     1201 ttgtgcaagt ctgagttgca gccgcgccca attcccggcg gcggcctgcc tgcctagtat
     1261 ccaggcacgt ccccggcatg accggtgcgc acccgcccca agctcaggag agcgccatgt
     1321 ccttgtcccg ccgtcaattt gtccaggctg ccctcgccgg tcctgccctc gcgctggatc
     1381 tgccggcgcg cgcggaggcg cgccgggtcg cgccgatcca ggccgtcgcg ctggacggct
     1441 tcaccacctt tgaccctcgc cccgtcgcag cgctcgccga gcgcctgttt ccgggccagg
     1501 gcgccgccct gaccacggcc tggcgcactc gtcagtttga gtactcgtgg ctgcgaaaca
     1561 gcatggaccg ctatgtggac ttctggcagg tgaccgagga ggccctggtc ttcgcggcac
     1621 gacagacccg cctcgaactc gacgggacgc ggcgccggca gctgatgcag gcctttctcg
     1681 cgctggacgc gtggccggac gcgcgtccgg ccctcgaacg tctccacgcc agtggcatca
     1741 ggctcgcctt cctctccaac ttcaccgtgg agatgcttga cgcggccagc cgcaacgcgg
     1801 gcctcgcggg gctgctggag ccccatctga gtaccgaccg ggttggcgtc ttcaagcccc
     1861 atccgcgggc ctaccggatg ggcgtggagg ctttcggcct gccgcgcgag gccatcgcat
     1921 tcgccgcctt cggaggctgg gatgcggccg gcgccaaggc ctttggctac ccgaccttct
     1981 gggtcaatcg gctcggtctg ccggtcgagc agctgggtgt gcagccggac ggtatcggcg
     2041 ccagcctgga ggatctggtg cgtttcgtca gtacctcgaa tgcagcgtcg cgtcccgcgg
     2101 cgtgagatca acccccctgg agaaccccat gaaaagtgtc cttgtttctg cgctgtgtgc
     2161 atccctgtca gtcctttccg cgccggtttc ggcccaggtc caggtgagcg ggcggagcgt
     2221 gcccctggcc attgccgtcg acgccgccgc ggcggcgctc aaacgctgcg aggcggatgg
     2281 ttaccgcgtc accgtcagcg tggtggacgc cgcgggggtc gaacgcgtcc gcctgcgcgg
     2341 cgatcacagc accatccaca ccagcgagac ggccttccgc aaggcctaca cggtgatcac
     2401 cctcggtccg atcttcaagg ccacgacgac cagcgcactc accgagcgga tcggcaagac
     2461 gcccaatctg ccggccttgt ccaccattga tcacgtcatc ttcctggccg gtggtgtggc
     2521 actgcaggcc cagggtgagt tcctgggcgc catcggcgtg ggcggggcgc ccggcggtgc
     2581 gctcgacgag cagtgtgccc aggccggcgt gcaggctgtc caggcccgac tcgacgggct
     2641 gggtgtgccg tcgaattgag cgggcgtccg gacgatcgac cctggtctcc atcgtctgct
     2701 ccggtcctca gccgccgtca ggcggctttt caccgaaaaa cgcccccggc gcccgggtgt
     2761 tcgagggcgc gccgggccgt tcgggtgggg cggcgcgacg cgaagcggct gtctcgtccg
     2821 gtctcgacgt cagccaccgt ccgggccggc gcctcgtcca ggcgtccgga ccggatggaa
     2881 cgcgtggggc cgggcgagcg tcggacgagg aggcgacgat cagccggcgg cacgttcagg
     2941 ggtgaagtgg cggaggatca gttcggccac gcgggcggcg tgctcttcga ggacgaagtg
     3001 gcccccatcg aggtagtgga cggccgccgc gggcaggtca ccgcgaaagg cttcggcgcc
     3061 ttcgacgaga aacagggggt ccccgcgccc ccaggcaatc agggtcttcg gctgccgctg
     3121 gcggaagtag gcgtgccaca ccgggtagag gtcgacgttg tgacggtagt cgcggaacag
     3181 ggccagctgg atctcggcgt tgccggggcg atccaggagg gcctggtcga agacccaggc
     3241 gtccgggctg acggccaggg ggtcgccggc gccggtgagg tactggaagc gggtggtggc
     3301 ggcgtccagc agcgcgcggg ccggtccttc gtcgccctgt tcccacagcg gcatgaagac
     3361 gtcacgcacc atcgggctga tgccttccag ataggcgttg gcgttctgga tcacgaggcc
     3421 gagcacgcgt tcggggtggg ccgtcgcgag gcgcagtccg accgggccgc cgtagtcctg
     3481 catgtagagg tggtagcggt ccacccccag atgagcgagc aggccgtcca cgacgccggc
     3541 cagcgcgtcg aagctgtagg ggaagcggcc ggtcgccggc gcgtcggaat ggccgaagcc
     3601 cgggtagtcc ggcgcgatga cgtggaagtg cccggcaaga tgggggatga ggtcgcggaa
     3661 catgtgggat gaggcgggaa agccgtgcag cagcacgatg gccggctgcg ccggatcgcc
     3721 tgccgagcgg tagaagatcc gcgtgccatt cacctcgtga tgggcgagac gaacggcagc
     3781 ggggtgggcg ggtgtctggc gggccgccag gaagggcgta tcggagttca tgggtgtcct
     3841 tgtgtcagga gatgacggga agcgttctcg taaccgtaaa aatgaaaatt aaaggttact
     3901 gacgcaacgc taatggtgta ctagtaactt gtcaacgatt tattttaagg ttacattctt
     3961 gtcgaatctg acgtactgag cgtcccatgt ctcctgcacc gactcctttc atctttgtcg
     4021 ccgacgacgt cgccctggat ttcgccaaca ccctggccgc ccccacgggc actttgttcg
     4081 actttctggg cgcgcccggc agcctgacgg cctgggcaca ggcttccggg gcgctgccgg
     4141 ctctgggcgc tttgcttgtc gcggcggcgg cgcacggtga attggccgag gtcgaggatg
     4201 aggcgcgggc gctgcgcgac tggctggtcg gcgtgctgcc gcagatccgc gcaggcagtg
     4261 ccccgttggc agctgtcgac ctcgcgccac tcaaccgtgc gctgacggcg gcctgctgtg
     4321 cgtggtcggt ccacgcgtgc gagggtcggg caaccctggc acgggactgg tgctacgccg
     4381 gtcccctgag tgcgctggcg cccctggccg aagccatcgc cagcctcctg gcccgcccgg
     4441 atctgcacga tatccgcaag tgcgaaaacc cccagtgcac cctgtggttc cgtgatgtga
     4501 ccaagcgcgg ccaccgacgc tggtgcagca ccgcggtgtg tggcaaccgg gccaaggtcg
     4561 cggcattccg ggatcgtcag aaacgcgcga cccgggagac ggacggagac ggctgaccgg
     4621 gcgacgggct ggcgtccttg c
//
LOCUS       DAOQMM010000157         4608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_11551_length_4608_cov_55.150890, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000157 DAOQMM010000000
VERSION     DAOQMM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11551_length_4608_cov_55.150890"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..2820
                     /locus_tag="PLH85_16390"
     CDS             67..2820
                     /locus_tag="PLH85_16390"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB66198.1"
                     /translation="MSGLVALAMVPATLGLGEHYLLDLVIAVPFVVGTLALTVRGLGW
                     QSPARRMAAWTGWGFFLAWLILLRFAAEWLLQAPWVVPVLSIVSSGASAWRMRALICA
                     VRGGGEVAEVTRVDQASALGGSFSVRLAALFFLSGFAALMYEVLFSKKLAVTFGSSSL
                     ATYTVLATYMGGMAIGSWLGGILAARGGDLLKRYVVCEGSIAAYCVLTPFLFDAVHDL
                     YAQWATNAPVDAGWVVALRFGLGALVLMVPTILMGMTLPILTACLDRGAAAYGRVVSV
                     LYGANTLGAALGALLAGYVLIPTLGLQSTTLLAALLNLLVAAFGGEMGKRLKQVGARL
                     EQDEDATSALSASGRALPGWLVLMSFGGVGLVCLGLETLYMHLLAVVAGNSTYAFSLM
                     LFAFLLGLGGGATVIRQIPQAISRPEVAMVVLLMLLAMCLGIGESQWSTMADYFASFA
                     GYPMPSSFGGRELVRAAVCLIALVPPAFLIGAFFPLAMGAVGRAGGVRALGTGAAVNT
                     LGNIAGVFIGGFVLLPTVGALGGIKVLAVLAMGLAVCIVIGAGLMRERVLVALGGMAA
                     LCVVAAPTDLDYTRLASGANVYFQPQHWGRVIDHAESADGGLTSVHVTLQDGGMTMKT
                     LTTNGKFQGNDSGEVEAQIGFALVPLAENPRRNDALVIGYGTGTTANTLKHAGFRQID
                     VVDLSRDVIRLANAHFAHLNERVTEAPGVKLHIADGRNYLLLQHRQYDLITVELTSIW
                     FAGAAASYNREFYQLARQRLRPGGVLQQWVQLHHLSGQDLLALLASVRSEFADVKVFH
                     FGGQGMIVARNGGAAPMAVGMRRPQDEPAIRAYSERILGHPLDDGPERLLDAQGVDNF
                     LSHWGEAEDWASTDDNMVLEYNTPKGNVLDGVRSYKENVTLMKAFGKAPVKRQDEQEG
                     GRH"
     gene            2817..3413
                     /gene="ampD"
                     /locus_tag="PLH85_16395"
     CDS             2817..3413
                     /gene="ampD"
                     /locus_tag="PLH85_16395"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNB66199.1"
                     /translation="MISPNCDTRPADCPVVLIVLHAISLPPDRFAGDGVERLFTNRLD
                     SAGHPYYAAIHHLRVSAHYFIRRTGEQICFVAPALRAWHAGVSSWRGRERCNDFSIGI
                     ELEGCDSLPFEPLQYEVLADLLATLCEQFPIEGITGHADIAPGRKTDPGPYFSWARLR
                     RLLTVRGGAALAARVEPESALDESVQMRWPFPSNRRPV"
     gene            3900..4598
                     /locus_tag="PLH85_16400"
     CDS             3900..4598
                     /locus_tag="PLH85_16400"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNB66200.1"
                     /translation="MFATRIVKYARHAASFTLHFTHGGLLVAGLIVTLFVASRIATHG
                     VQGLALGSLLGSSPVVAAQAPDSEVQDQTEEVGQEIALSPDLQRVKSYLAKRYQVSAV
                     AIEPLLASAQQTGRSVGVDPLLLVAVMAIESRFNPFAESPVGAQGLMQVIPRFHTDKV
                     DVKSDKAALFDPDTNIRVGALVLKEYIRNTGSVERALEQYGGSSDPATPYAIKVMAVK
                     AQLAAAARGKIIGA"
BASE COUNT          712 a         1272 c         1624 g         1000 t
ORIGIN      
        1 cacctgggct ggagcttggc cgcgtggttg gtgtccctgc cgctacggaa tgggactatc
       61 aaatgggtga gcggtctggt ggccctggcc atggtgccgg ccacacttgg actcggcgag
      121 cactatttgc ttgatctggt gattgccgtt ccgtttgtgg tcgggacgct ggcattgact
      181 gtccgtgggc taggttggca gtctccagcc aggcgtatgg ctgcatggac gggctgggga
      241 ttttttttag cgtggctgat tctgttacgt ttcgcggcgg aatggttgct gcaggcaccc
      301 tgggtggtgc cggtgctttc gatcgtgtcg tcgggtgcgt cggcctggcg gatgagggcg
      361 ttgatatgcg ccgtgcgcgg ggggggcgaa gtcgcggaag tcacacgcgt agatcaggct
      421 tcggcgctcg gcgggtcttt cagtgtgcgt ctggcagcgc tgttttttct gtccggcttt
      481 gcggcactga tgtacgaggt gctgttttcc aagaagctcg cggtgacgtt cgggagttcg
      541 tcgctggcaa cctataccgt gctggccacc tacatgggtg gcatggcgat cggctcttgg
      601 ctggggggga tcctggctgc ccgcggggga gatttgctga agcgctacgt ggtgtgcgag
      661 gggagcatcg cggcgtattg tgtgctgacg cccttcttgt ttgacgcagt tcacgatctg
      721 tatgcccagt gggcaaccaa tgcccccgtc gacgccgggt gggtggtggc cttgcgattc
      781 gggctgggtg ctctggtcct gatggtgccg acgatcctga tgggcatgac gctgcccatc
      841 ctgacggcct gtctcgaccg gggcgcggcg gcctatggac gtgtcgtgtc cgtgctttac
      901 ggcgccaata ccctgggcgc cgcgctgggg gcgttgctcg cgggctatgt gctgatcccg
      961 accctgggcc tgcaatcgac caccctcctt gctgcgctgt tgaatcttct tgtcgcggct
     1021 tttggcgggg agatgggcaa acgactgaag caggtggggg cccgtctgga gcaggacgaa
     1081 gatgccacat ccgctctgtc cgccagcggc cgcgccttgc cggggtggct ggtcctgatg
     1141 agcttcggag gcgtgggcct ggtatgcctg gggcttgaga cgctgtacat gcacctgctg
     1201 gcggtggtgg ctgggaacag tacctacgcg ttctctctga tgctgtttgc cttcctgctg
     1261 gggttgggcg gtggggcaac ggtcattcgg cagattccgc aggcaatcag ccggccggaa
     1321 gtggccatgg tggtattgct gatgctgttg gctatgtgtc tgggcatcgg ggaaagccaa
     1381 tggagcacca tggcggacta cttcgcgtca tttgcggggt atccgatgcc gagcagcttt
     1441 ggtgggcggg agctggtgag agcggctgta tgcctgatcg cgctggttcc gccggcgttc
     1501 ctcatcgggg cctttttccc gctcgccatg ggggccgtcg gacgtgccgg tggcgtgcgg
     1561 gcgcttggga ctggtgcagc cgtcaatacg ctgggcaata tcgccggggt cttcatcggt
     1621 ggcttcgtgc tgctgccgac ggttggtgcg ttgggtggca tcaaggtgct ggcggtcctc
     1681 gcaatggggc ttgccgtgtg catcgtcatc ggggcgggcc tgatgcggga gcgggtactt
     1741 gttgcactgg gggggatggc cgcgctctgt gtcgttgcgg cgccgacgga tctggactac
     1801 acgcggctcg cgtcgggagc caatgtctat ttccagcctc agcactgggg gcgggtcatt
     1861 gatcacgcgg agagcgcgga cggcgggttg acatcggttc atgtgacgct gcaggacggc
     1921 ggcatgacca tgaagacgct gacgacaaat ggaaaattcc agggcaatga ttccggcgaa
     1981 gtggaggcac agatcgggtt tgccctggtt ccgctggccg agaatccccg caggaatgat
     2041 gcgctggtga tcggctacgg taccgggacg accgcgaata ccctgaagca cgcgggcttc
     2101 cgtcagattg acgtggtgga tctgagtcgg gatgtgatcc gtctcgccaa cgcccatttc
     2161 gcccatctga acgagcgtgt gacggaggcg ccaggggtga agctgcacat tgcggatggc
     2221 cgcaattatc tgttgctcca gcatcggcag tacgacctga ttacggtgga gctcacctcg
     2281 atctggtttg ccggtgctgc ggccagctac aaccgcgagt tttaccagtt ggcgcgtcag
     2341 cgtctgcgcc ctggcggcgt cctgcagcag tgggtgcagt tgcatcattt gtccggacag
     2401 gatctgctcg cgttgctggc atcggtgcgt tccgaatttg cggacgtgaa ggtcttccat
     2461 tttggcgggc agggcatgat cgtcgcccgc aatggggggg cggcgcccat ggccgtcggc
     2521 atgcgccggc cacaggatga accggcgatc cgggcctaca gcgaacgcat cctggggcat
     2581 ccgctggatg acgggccgga aaggttactc gatgcccagg gcgtcgataa cttcctgtcc
     2641 cattggggcg aagcggaaga ctgggcgtct accgatgaca acatggtcct cgagtacaac
     2701 acgcccaagg gcaacgtcct cgacggcgtg aggtcataca aggagaacgt caccctgatg
     2761 aaggctttcg ggaaggcgcc ggtcaagcgt caggatgagc aggaaggggg acggcattga
     2821 tttcccccaa ctgcgatacc cgtccagccg actgccccgt tgtattgatc gtacttcacg
     2881 ccatcagtct gccgccggat cggttcgcag gcgacggcgt ggagcggttg ttcaccaatc
     2941 gtcttgattc cgccgggcat ccctattacg cggcgatcca tcatctgcgg gtgtcggccc
     3001 attacttcat tcgccgcacg ggtgagcaga tctgcttcgt tgcgccagcg ttgcgggcct
     3061 ggcacgcggg ggtgtcgagc tggcgggggc gggagcggtg caatgatttc tctatcggaa
     3121 tcgaacttga ggggtgcgac agcctgccct ttgagccgct tcagtacgag gtgctggccg
     3181 acctgctcgc gacgctgtgc gagcagtttc cgatcgaggg gatcacgggg catgcggaca
     3241 ttgcgccggg ccgcaagacc gaccctggtc cgtacttctc gtgggcgcgc ttgcggcggc
     3301 ttctgaccgt tcggggcggg gctgcgctgg cggcgcgggt ggagccggaa tccgccctcg
     3361 acgagtccgt gcagatgcgc tggccgttcc cgtcaaacag gcgtccggtc tgaattcatc
     3421 ggggtgagcg ggtgcccgga atgcgggatg aggcgccatc tgaccacgct ccgcgagacc
     3481 ccggattatg ctaatgagat tgcatgtcga tggcctcggg cgcggggcga ttgacaaaac
     3541 cctgttgaat caatacaatg caaattgtgt gcagtgcagc acgcataaaa gaaatttaac
     3601 gcccgtgccg gggcagcgaa tttcagcggg tttggcccgc ttcgctgttg ttgttccgac
     3661 actccccatg tcggattccg gtacgttttt ggcacgctgt ctggttgcat cagcgtgttt
     3721 ggtcaaccac tggtgaccgg gcttttcaga gcgccgccgg ccgttccagg ccggtgagtc
     3781 gaatcaggct ccgttgttca ggagccctgg tgaagcagag cggactcctc ttcctgtctt
     3841 agggcaggcg tgggtgtgcg tgtgcgttcg aaagggacgg ccgcaagaaa ggagaaccca
     3901 tgttcgcaac tcgtatcgtg aagtacgcac gtcacgcggc ttcattcacg cttcacttca
     3961 cccacggcgg gttgctggtc gccggtctta tcgtcacact cttcgtcgcc tcccggattg
     4021 ccactcatgg tgtgcagggt cttgcgcttg gttccctgct ggggagttct cccgtggtag
     4081 cggcacaggc gccggacagc gaggtgcagg accaaacgga ggaggtgggc caggaaatcg
     4141 ccctgagccc ggacctgcag cgggtcaaga gctatctggc caaacgctac caggtgtcgg
     4201 cggtggcgat cgagccgctt ctggcgtcgg cgcagcagac ggggcgcagc gtgggtgtcg
     4261 atcccttgct gctggtggcg gtgatggcca tcgaatcgcg tttcaatccg tttgcggaaa
     4321 gcccggtggg tgctcaggga ctgatgcagg tgattccccg cttccacacg gacaaggtgg
     4381 atgtcaaatc tgacaaggcc gccctgttcg atccggacac gaatatccgg gtcggagcgc
     4441 tggtactgaa ggagtacatc cgcaacacgg gttcggttga gcgggcgttg gagcagtacg
     4501 gtggctccag cgatccggct acgccctacg ccatcaaggt catggcggtg aaggcccagc
     4561 ttgcggccgc agcccggggc aagatcatcg gcgcctgatc ctttttag
//
LOCUS       DAOQMM010000158         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_12159_length_4465_cov_38.379138, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000158 DAOQMM010000000
VERSION     DAOQMM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12159_length_4465_cov_38.379138"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLH85_16405"
     CDS             complement(<1..341)
                     /locus_tag="PLH85_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66201.1"
                     /translation="MTQRDIELALQLVREALPHLAVPRRLCTRRLKPGSRAMGQYRWL
                     SDTLRLNPRYLARLSDPDALDLLDTILHELLHKASPLWKQVRDTFRPHPDIWLDAERL
                     TAQLGPRFLHLR"
     gene            complement(349..669)
                     /locus_tag="PLH85_16410"
     CDS             complement(349..669)
                     /locus_tag="PLH85_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HNB66202.1"
                     /translation="MLVIFKSPASGDVIMFEKNGKEMLGVLGKDPADAKGIVTVEQLP
                     GAIATLRQAIADDKARHAGQDDVQEAEPKSGGPGVSFFQRAVPLLDLMERSLKDKVPV
                     TWGV"
     gene            800..1699
                     /gene="rarD"
                     /locus_tag="PLH85_16415"
     CDS             800..1699
                     /gene="rarD"
                     /locus_tag="PLH85_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNB66203.1"
                     /translation="MNPPPDRRTTLAGIACGLAAYGMWGFFPLFFRQLGHVSPVDLLC
                     NRALWGCVFVSLILTVRRAWAGPLAAARRPGTLPRLTLAALLVGANWLAFLWAVDQHR
                     VIEGSLGYFLTPLVNVLLGLVVLKERLKRLEWASVALAVAAVGNEFLSLGSLPWVSLF
                     LGGTFGLYGLVRKQVPVDAVSGLWLETLSLMPLILLYAGWQTSRGHGVFTGFDASTQI
                     LLAVAGVMTALPLMFFAAATQRLNLATVGMLMYINPTLQFLTAVLIFGEALRPARLVT
                     FALIWAGLLLYSWSGWQKYRAAR"
     gene            complement(1701..>4465)
                     /locus_tag="PLH85_16420"
     CDS             complement(1701..>4465)
                     /locus_tag="PLH85_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB66204.1"
                     /translation="APTAVLAEGKGQTATADAGADAPPKRRTCLVITGFGRKTDPATG
                     RVLDLDQSYAMIRSAVEAAGMACVRADGGEATVLGAAHLFQQILASDLVICDLSCRDG
                     NTLYLAGVSLALRPRGILLIADDGFAEYASAAAPRLGLTYASAGDALAPDVQARFSAS
                     LRALVDGLFSAELDLPGSQHRPLGSPLYASGHLQPPRMLDAPSPAAAWAAPPSGSERL
                     CFIVQGAGTKTDLTTGRTLDLDATYAVVRDAVTSVGLTPRRGDEVVHSGHLESDLHDF
                     LLDAPFVISDLTAGLPEVLIQLGLRHGLRPGRTLLIAEEQFKLPVELASLRILRYKHL
                     GTDIGAQEARRLQAAIVDWLKAAMASPTVDSPVYAALPTLRPPQRIEAADPPDAEPAM
                     AEAPPEAGAASGPAADEAAPAPPPRVFLSHYQGYLQYALPLAKALHSVGIEVSWYMNL
                     SSGEDFAETLALWLEESDVVIAVVGRQTSNAKNALNEIHRAIALGKRLVPVFVDEGAP
                     SEVLSFMAANVDASGHIAYLAGLKDLALDQALAHAAVRIAAALKPAAPTPEPAAEDAP
                     AEPAATSADPMPLIWEITIRQRASGRLHFRVTTADGQHEWQVGYQPALVQPLLNALQT
                     STRLDVQAAQTLAHLLIPVEWARMAPRTAYRLTVGRETAALPWELLLEDTLWTRELRD
                     PPRVLRHLDGLRRNPGVALASGSTRALVIGDPDTSGMSTTHPPARLPGARQEAETVAD
                     TLEEAGFQVTRSLGEGASAVIGKLYGAPFRLLLASGSAVRDYALPDGNRVTGLALGDD
                     VFLTVHELRQLRQLPEVVVLNACHLGQVGADAESATADLVPDLLQAGVPVVVAPAWSV
                     DDMAAAAFSKAFFAALAGNARLEDAVANARIAAARAAPETSTWAAYQAWGDPDFRLFP
                     TAPAAE"
BASE COUNT          606 a         1444 c         1695 g          720 t
ORIGIN      
        1 cgcaggtgca ggaagcgcgg ccccagctgg gcggtgagac gttcggcgtc cagccagatg
       61 tccgggtggg ggcggaaggt gtcgcggacc tgcttccaca ggggcgaggc cttgtggagc
      121 agttcgtgga ggatggtgtc gaggagatcc agcgcgtccg gatccgacag gcgcgccagg
      181 tagcgcgggt tgaggcgcag ggtgtcggac agccagcggt actggcccat ggcccggctg
      241 ccgggcttga ggcggcgggt gcataggcgg cgcggcacgg cgaggtgggg cagcgcctcg
      301 cggaccagct gcagggccag ttcgatgtcg cgctgggtca tggtggtgtc acacgcccca
      361 ggtgaccggc accttgtcct tgagggagcg ctccatcagg tccagcagcg gcaccgcgcg
      421 ctggaagaag ctcacccccg gcccgccgga cttgggctcg gcttcctgga cgtcgtcctg
      481 gccggcatgg cgggctttgt cgtcggcaat ggcctggcgc agcgtggcga tggcgccggg
      541 gagctgctcg acggtgacga tgcccttggc gtcggcggga tccttgccga ggacgccgag
      601 catctccttg ccgttctttt cgaacatgat cacgtcgccg ctggcggggc tcttgaagat
      661 gaccaacatg gtgtgcgcgt cctcgaaagg aaatggatgc gttcattgta gggctttgtg
      721 tcccgatggc ggaaaaccct gagggtgcaa tctggcggac ggtggtaccg tgcagggccg
      781 tcccccgcgc gagcgccccg tgaatccgcc tcccgaccgc cgtacgaccc ttgccggcat
      841 tgcctgcggc ctcgccgcct atggcatgtg gggcttcttt cccctgttct tccgccagct
      901 cggccacgtc tcgccggtgg atctgctgtg caaccgggcc ctgtggggct gtgtcttcgt
      961 gagcctgatc ctcaccgtgc ggcgcgcctg ggcggggccc ctggcggcgg cgcggcggcc
     1021 gggcacgctg ccgcgcctga ccctcgccgc gctgctggtg ggggccaact ggctggcctt
     1081 cctgtgggca gtggatcagc accgggtcat cgaaggcagc ctgggctact tcctgacgcc
     1141 gctggtgaac gtgctgctgg gcctggtggt gctcaaggaa cgcctcaagc gcctggaatg
     1201 ggcctcggtg gcgctggccg tggcggcggt gggcaacgag ttcctcagcc tgggcagcct
     1261 gccctgggtg tccctgttcc tcggcggcac cttcggcctg tacggcctgg tgcgcaagca
     1321 ggtgccggtg gatgccgtct ccggcctgtg gctggagacc ctttcgctga tgcccctgat
     1381 cctgctctac gccggctggc agacgagccg cgggcatggg gtgttcaccg gcttcgacgc
     1441 ctccacccag atcctgctgg cggtggcggg cgtcatgacg gccctgccgc tgatgttctt
     1501 cgcagccgct acccagcgcc tcaacctggc caccgtgggc atgctcatgt acatcaaccc
     1561 gaccctgcag ttcctcaccg cggtgctgat cttcggcgag gccctgcgcc cggcgcggct
     1621 ggtgaccttc gccctgatct gggccggatt gctgctctac agctggagcg gctggcagaa
     1681 gtaccgggcg gcgcgctgat tcattccgcc gccggggcgg tcgggaagag gcggaaatcc
     1741 gggtcgcccc aggcctggta ggccgcccag gtggaggtct ccggtgcggc ccgggcggca
     1801 gcgatgcggg cgttggcgac ggcatcctcc aggcgcgcgt tgccggcgag ggcggcgaag
     1861 aaggctttgc tgaaggccgc cgcggccatg tcatccaccg accaggcagg ggccaccacc
     1921 accggaacgc cggcctggag caggtctggc accagatcgg cggtggcgga ttcggcgtcc
     1981 gcgcccacct gacccaggtg gcaggcgttg agcaccacga cctccgggag ctgcctgagc
     2041 tggcgcagct cgtgcaccgt caggaagaca tcgtcgccca gggccaggcc ggtgacccgg
     2101 ttgccgtcgg gcagggcgta gtcccgcacg gcgctgccgc tggccagcag caggcggaag
     2161 ggcgcgccgt agagcttgcc gatcaccgcc gaggcgcctt cgccgaggct gcgggtgacc
     2221 tggaagccgg cctcttcgag ggtgtcggcg acggtctccg cctcctgacg tgcgccgggc
     2281 aggcgggcgg gcggatgggt cgtcgacatg cccgaggtgt ccgggtcgcc gatgacgagg
     2341 gcgcgggtgg acccgctggc gagggccacg ccggggttcc ggcgcaggcc atcgaggtgg
     2401 cgcaggaccc gcggcggatc gcgcagttca cgggtccaca gggtgtcctc gagcagcagc
     2461 tcccaaggca gcgccgcggt ctcgcgaccc accgtcaggc ggtaggccgt gcgcggcgcc
     2521 atgcgcgccc actcgaccgg gatcagcagg tgggcgaggg tctgggccgc ctggacgtcc
     2581 aggcgtgtgg acgtctgcag cgcgttcaac agcggctgca ccagtgccgg ctggtagccg
     2641 acctgccatt cgtgttgccc gtcggcggtg gtgacccgga agtgcaggcg gccgctggcc
     2701 cgctggcgga tggtgatttc ccagatcagc ggcattggat ctgcagacgt ggcggcgggc
     2761 tccgcggggg cgtcctcggc ggcaggttcc ggcgtgggtg ccgcgggctt cagcgctgct
     2821 gcgatgcgca ccgcggcatg ggcgagggcc tggtccaggg ccaggtcctt gaggccggcg
     2881 aggtaggcga tgtggcccga ggcgtccacg ttggcggcca tgaaggacag cacttccgac
     2941 ggtgcgccct cgtccacgaa gaccggtacc aagcgcttgc cgagcgcaat tgctcgatga
     3001 atctcattca atgcgttctt ggcattcgag gtctgacggc cgactacggc aatcaccacg
     3061 tcactttctt ccagccagag ggccaaggtc tcggcgaagt cttcaccaga gctcagattc
     3121 atgtaccacg atacttctat gcccacgctg tgtagcgcct tggccagagg caaagcgtat
     3181 tggaggtagc cttggtagtg ggacaggaag acgcgaggcg gcggtgcggg cgccgcttcg
     3241 tccgcggccg ggccggaggc ggccccggct tccgggggcg cttcggccat cgcgggctcg
     3301 gcgtcgggcg ggtcggcggc ctcgatgcgc tgcggcgggc ggagcgtggg cagggcggca
     3361 tagaccgggc tgtcgacggt gggcgaggcc atcgcggcct tgagccagtc gacgatggcg
     3421 gcctgcaggc ggcgcgcttc ctgggcaccg atgtcggtgc ccaggtgctt gtagcggagg
     3481 atgcggaggg aggcgagttc gacggggagc ttgaattgtt cttcggcgat cagcagtgtc
     3541 cggccgggac gcaggccgtg gcggaggccg agttgaatca gcacctccgg cagaccggcg
     3601 gtgagatcgg agatgacgaa gggggcgtcc agcaggaagt cgtggaggtc ggattcgagg
     3661 tgcccggaat ggacgacctc gtcgcctcgc cggggcgtca ggccgacgct cgtcacggca
     3721 tcgcggacaa ccgcgtaggt ggcgtcgaga tccagtgtcc gtccggtcgt gagatcggtc
     3781 ttggtgcccg cgccctgcac gatgaagcac agccgttcgc tgccgctggg tggagccgcc
     3841 cacgcggcgg caggcgacgg cgcgtcgagc atccggggcg gctggaggtg tcctgaggca
     3901 tagagcggac tgcccagggg ccggtgctgg gaccccggca gatcgagttc ggcgctgaac
     3961 aggccgtcga cgagcgcgcg cagcgatgcc gagaagcgcg cctggacgtc cggcgcgagg
     4021 gcgtcgcctg cggaagcgta ggtgaggccc aggcgcggcg cggcggctga ggcgtactcg
     4081 gcgaagccat catcggcgat cagcaggatg cccctcggac gcagcgccag gctcacgccg
     4141 gccagataaa gggtgttgcc atctcggcag ctcaggtcgc agatcaccag atcgctggcg
     4201 aggatctgtt ggaacagatg cgcggcgccg aggacggtgg cttcaccgcc gtcggcccgt
     4261 acgcacgcca ttccggccgc ctccacggcg ctgcggatca tcgcgtaact ctggtccagg
     4321 tcgaggacgc ggcccgtggc gggatcggtc ttgcgcccga aacccgtgat gaccaggcag
     4381 gttcgccgct tcggcggcgc gtccgccccg gcgtccgccg tcgcggtctg ccctttcccc
     4441 tccgccaata ccgccgtcgg cgccc
//
LOCUS       DAOQMM010000159         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_12511_length_4388_cov_44.377106, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000159 DAOQMM010000000
VERSION     DAOQMM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12511_length_4388_cov_44.377106"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..288)
                     /locus_tag="PLH85_16425"
     CDS             complement(151..288)
                     /locus_tag="PLH85_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66205.1"
                     /translation="MTACRLAARLERTLGDGCVDVALTDPTAAEAPFHRIARSEEGPL
                     L"
     gene            complement(285..452)
                     /locus_tag="PLH85_16430"
     CDS             complement(285..452)
                     /locus_tag="PLH85_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66206.1"
                     /translation="MRLTVDEHGLIREGTRQEIGPDRTVRLFGSRLDDSRRGGDIDWL
                     VERPQPAADRR"
     gene            complement(500..4201)
                     /gene="metH"
                     /locus_tag="PLH85_16435"
     CDS             complement(500..4201)
                     /gene="metH"
                     /locus_tag="PLH85_16435"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNB66207.1"
                     /translation="MQADRSTELQSLLTHRILILDGAMGTMIQQHRLGEADYRGARFA
                     EHPRDLKGNNDLLVLTRPDVVGGIHRAYLEAGADIIETCTFNATRVSQAEYGLAGLAY
                     ELNVEGARLVRALCDEFTAANPAKPRFCAGVIGPTSRTLSISPDVNDPGFRNIDFDAL
                     ADDYHEAARGLLDGGADILLIETVFDTLNAKAAVFAVEKLFAERGQRVPVMISGTITD
                     ASGRTLSGQTAEAFWNSLRHARPISFGLNCALGARELRPYVEELANTCDCFVSAHPNA
                     GLPNPLSPTGYDETPEQLAAEIVEWARSGLVNIVGGCCGTSPAHIRAIAEAVRLVTPR
                     SVPEVAPRLRLSGLEPFNVGPDSLFVNVGERTNVTGSRAFARMILEGRFDDALAVARQ
                     QVENGAQVIDINMDEAMLDSLAAMERFLKLIAAEPDISRVPVMLDSSKWSVIEAGLKC
                     IQGKGVVNSISMKEGEAEFLRQARLCRQYGAAVIVMAFDEKGQADTYARKTEICRRAY
                     DLLTGIGFPAEDIIFDPNIFAIATGIEEHDNYAVDFIEATRWIRQHLPHAQVSGGVSN
                     VSFSFRGNDAVREAIHTVFLYHAIRAGMSMGIVNAGMLGVYDDLAPELRDKVEDVVLN
                     RHPGAGEALVEFAQTVKEGRSKDAGPDLSWREQPVEARLSHALVKGITDFVVADTEEV
                     RARLEAAGQPPLAVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFI
                     EAEKLRTGAASKGRIVIATVKGDVHDIGKNIVGVVLGCNGYDVVDLGVMVSADKILHA
                     AREHGAQAIGLSGLITPSLEEMSHVAAEMQRQGFDVPLLIGGATTSRAHTAIKIAPHY
                     DQPVVYVPDASRAVGVVTRLLSDTQRSSFTAELTADYAKIRAQHAQKKGVALVPLGEA
                     RANAFRWNADPAYVPPVPARPGLHAFDIDLAELVDYIDWGPFFQTWDLAGRYPDILQD
                     ELVGETARELKSDAEVMLARMVAEQDTAPWVRARAVFGLFPAHAAGDDIVVYTDEARQ
                     DVRMTLHGLRQQHERPAGKPHYALADFVAPRDSGVADYVGAFAVTAGLDIEGRLAAYE
                     RSHDDYHAIMLKALADRFAEAAAEWLHARVRREYWAYAPAEALSNDELIREAYRGIRP
                     APGYPACPDHTVKGDLFRLLDAPAHAGMALTESFAMTPAASVCGLYLAHPDARYFAIS
                     KIGRDQLEDWARRKGMAVADAERWLAPLL"
     regulatory      complement(4271..4342)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
BASE COUNT          601 a         1402 c         1687 g          698 t
ORIGIN      
        1 tccatctggc agcacttcgg gctgaaggcg ccgggcggac cggggttgtg ggaggcgtag
       61 gccggccgcg tcaggccgcg cccgcacccg ggaaacgact cctgatgtaa gcggccaggc
      121 tcgccagcat gccggcgagg acggccgttc tcacagcagc gggccttctt cagaccgggc
      181 gatgcgatgg aagggggctt ctgcggcggt gggatcggtc agcgccacgt ccacgcagcc
      241 gtcgcccagg gtacgttcca ggcgggccgc gaggcggcag gcggtcatcg acgatccgcc
      301 gcgggctgcg ggcgctccac cagccagtcg atgtcgccac cccgccggct gtcatccagg
      361 cgcgatccga acaagcgtac ggtcctgtcc ggcccgattt cctggcgggt gccttcgcgg
      421 atcaggccgt gctcgtcgac agtcagacgc acgcgggggc tcgggcagca gaaacgctct
      481 ggagtctggc ccttcccgct cacagcagcg gcgccagcca gcgctctgcg tccgccaccg
      541 ccatgccctt gcgccgggcc cagtcttcca gctggtcgcg gccgatcttg gagatggcga
      601 agtagcgggc gtccgggtgg gccaggtaga ggccgcagac ggaggcggcc ggggtcatgg
      661 cgaagctctc ggtgagggcc atgccggcgt gggcgggggc gtcgaggagg cggaacaggt
      721 cgcccttgac ggtgtggtcc gggcaggccg ggtagccggg ggcggggcgg atgccgcggt
      781 aggcttcgcg gatcagttcg tcgttgctca gggcctcggc cggggcgtag gcccagtatt
      841 cccggcgcac gcgggcgtgc agccactcgg cggcggcttc ggcgaagcgg tcggcgaggg
      901 ccttgagcat gatcgcgtgg tagtcgtcgt ggctgcgttc gtaggccgcc aggcggcctt
      961 cgatgtccag gccggcggtg acggcgaagg cgcccacgta gtcggccacg ccgctgtccc
     1021 gcggcgcgac gaaatccgcc agggcgtagt ggggcttgcc ggcggggcgt tcgtgctgct
     1081 ggcgcaggcc gtgcaaggtc atccgcacgt cctggcgcgc ctcgtcggtg tagacgacga
     1141 tgtcgtcccc cgccgcgtgg gccgggaaga ggccgaagac ggcgcgggcg cgcacccacg
     1201 gcgcggtgtc ctgctcggcc accatgcggg cgagcatcac ctcggcgtcg gacttgagct
     1261 cgcgggcggt ttcgcccacc agttcgtcct ggaggatgtc cgggtagcgg ccggccaggt
     1321 cccaggtctg gaagaagggg ccccagtcga tgtagtccac cagctcggcg aggtcgatgt
     1381 cgaaggcgtg gagacccggc cgggcgggca ccggcggcac gtaggccggg tcggcgttcc
     1441 agcggaaggc gttggcgcgg gcctcgccca ggggcaccag cgcgacgccc ttcttctggg
     1501 cgtgctgggc gcggatcttg gcgtagtcgg cggtcagctc ggcggtgaag ctgctgcgct
     1561 gggtgtcgga gagcaggcgc gtgaccacgc ccacggcgcg ggaggcgtcg ggcacgtaga
     1621 ccaccggctg gtcgtagtgg ggcgcgatct tgatggcggt gtgggcgcgg ctggtggtgg
     1681 cgccgccgat cagcaggggt acgtcgaagc cctggcgctg catctcggcg gccacgtggc
     1741 tcatctcttc cagcgacggg gtgatcaggc cggacaggcc gatggcctgg gcgccgtgct
     1801 cgcgggcggc gtggaggatc ttgtcggcgg acaccatgac gcccaggtcc accacgtcgt
     1861 agccgttgca gccgaggacc acgccgacga tgttcttgcc gatgtcgtgc acgtcgccct
     1921 tgaccgtggc gatgacgatc cggcccttgc tggcggcgcc ggtgcgcagc ttttcggcct
     1981 cgatgaaggg gatcaggtgg gccaccgcct gcttcatgac gcgggcggat ttgaccacct
     2041 ggggcaggaa catcttgccg gcgccgaaga ggtcgcccac cacgttcatg ccggccatca
     2101 gggggccttc gatgaccgcc aggggcggct ggccggcggc ttcgagccgg gcgcggactt
     2161 cctcggtgtc ggccaccacg aagtcggtga tgcccttgac gagggcgtgg gacaggcgcg
     2221 cctcgacggg ctgctcgcgc caggacagat ccgggccggc gtccttgctg cggccttcct
     2281 tgacggtctg ggcgaactcc accagcgcct cgccggcgcc cgggtggcgg ttgaggacca
     2341 cgtcctccac cttgtcgcgc agctcgggcg ccaggtcgtc gtagacgccc agcatgccgg
     2401 cgttgacgat gcccatgctc atgcccgccc ggatggcgtg gtacaggaag acggtgtgga
     2461 tggcctcgcg caccgcgtcg ttgccgcgga agctgaagct cacgttggag acgccgccgc
     2521 tcacctgggc gtggggcagg tgctggcgga tccagcgggt ggcctcgatg aagtccaccg
     2581 cgtagttgtc gtgctcctcg atgccggtgg cgatggcgaa gatgttgggg tcgaagatga
     2641 tgtcctcggc cgggaagccg atgccggtga gcaggtcgta ggcgcgtcgg cagatctcgg
     2701 tcttgcgggc gtaggtgtcg gcctggccct tctcgtcgaa ggccatcacg atcaccgcgg
     2761 cgccgtactg gcggcacagg cgcgcctgac gcaggaattc cgcctcccct tccttcatgg
     2821 agatggaatt gaccacgccc ttgccctgga tgcacttgag gccggcctcg atcacgctcc
     2881 acttggagga atcgagcatc acgggcaccc gggagatgtc cggctcggcg gcgatgagct
     2941 tgaggaagcg ctccatcgcg gccagggagt cgagcatcgc ctcgtccatg ttgatgtcga
     3001 tcacctgggc gccgttttcc acctgctggc gggccacggc cagggcgtcg tcgaagcggc
     3061 cttccaggat catccgcgca aaggcgcggg agccggtgac gttggtgcgc tcgcccacgt
     3121 tcacgaagag gctgtcaggg cccacgttga agggctccag gcccgacaga cgcaggcgcg
     3181 gggccacctc cggcacgctg cgcggggtga ccaggcgcac cgcctcggcg atggcgcgga
     3241 tgtgggccgg cgaggtgccg cagcagccgc cgacgatgtt caccagcccg ctgcgcgccc
     3301 actccacgat ctcggcggca agctgctccg gcgtctcgtc gtagccggtg ggcgacaggg
     3361 gattgggcag gccggcgttg gggtgggccg acacgaagca gtcgcaggtg ttggccagct
     3421 cttccacgta ggggcgcagc tcgcgggcgc cgagggcgca gttgaggccg aagctgatgg
     3481 gccgggcgtg gcgcagggaa ttccagaagg cctcggcggt ctggcccgag agggtgcgcc
     3541 cggaggcgtc ggtgatggtg ccggagatca tcaccggcac gcgctggccg cgctcggcga
     3601 agagcttctc cacggcgaac accgcggcct tggcgttgag ggtgtcgaac accgtctcga
     3661 tgagcaggat gtcggcgccg ccgtccagca ggccgcgggc ggcctcgtgg tagtcgtccg
     3721 ccagggcgtc gaagtcgatg ttgcggaagc ccggatcgtt cacgtccggc gagatggaca
     3781 gggtgcgcga ggtgggcccg atcacgccgg cgcagaagcg cggcttggcc gggttggcgg
     3841 cggtgaattc gtcgcacagg gcgcgcacca ggcgggcgcc ttccacgttg agctcgtagg
     3901 ccagcccggc cagaccgtat tccgcctggg acacgcgggt ggcgttgaag gtgcaggtct
     3961 cgatgatgtc cgcaccggcc tccaggtagg cgcgatggat gccgcccacc acgtccggac
     4021 gggtcagcac cagcaggtcg ttgttgccct tgagatccct gggatgttcc gcgaagcgcg
     4081 cgccccggta atcggcctcg cccagccggt gctgctggat catggtgccc atcgccccgt
     4141 cgaggatcag gatgcggtgg gtcagcaggg actggagttc ggtgctgcgg tcggcttgca
     4201 tggcggttcc ttcgggttca gccggatgca cgcgcgctca aacgcaaacg gcgccacaaa
     4261 cccggctgtc ggtttgtgag cgccgttgaa tcggttgccg agcctggccc gcgggtgacg
     4321 ggtcgcaacg ctcctcggca aggggcggat tttacggggt ttttggcggg gctgccaagg
     4381 gtgtgcgg
//
LOCUS       DAOQMM010000160         4349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_12677_length_4349_cov_47.908244, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000160 DAOQMM010000000
VERSION     DAOQMM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12677_length_4349_cov_47.908244"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="PLH85_16440"
     CDS             <1..1798
                     /locus_tag="PLH85_16440"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNB66208.1"
                     /translation="TPAALRALCLERAAQALEARSHALVALLVREAGKTFAAGLGEVR
                     EAVDFCRYYAARLRAGDLIGSAPLGPVVAISPWNFPLAIFCGQIVAALAAGNPVIAKP
                     AEQTPLIADQAVALLHEAGIPRGVLQCLPGPGATVGAALVADARVAAVMFTGSTEVAT
                     AIHRVLADRGDVPLIAETGGLNAMIVDSTALPEQVVTDVIASAFDSAGQRCSALRLLC
                     LQDEIAPRVLELLDGALGELRIGPPEDFATDIGPVIDAAAHLQLDAYVDALRADGHCV
                     MTRSLPDVCTDGHFVMPTVIELRTPTELQREVFGPVLHVVRFALHDLDALLDALGRSG
                     YGLTRGIQSRVDSTVERILGHGGVGNVYVNRNMIGAVVGVQAFGGEGLSGTGPKAGGP
                     LLLRRLVRRAPGLSLPGQCAPADLDTLDAWLTAGAGGVVDDAARARLRVRVAACRAAT
                     LWGAVLDLPGPTGERNTWRVHPRGMVLGLAEAVEDAVHQLAAALATGNRLAVPAAAAG
                     WLSALPEALRGCVALRPSDGFEPWQAALLAGAEGRVAAWRRQLAAQPGPLRPILVADP
                     DYVLERLLVERCVTVNTAATGGNAGLMGLGES"
     gene            1850..3589
                     /locus_tag="PLH85_16445"
     CDS             1850..3589
                     /locus_tag="PLH85_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNB66209.1"
                     /translation="MPYRACLRLSTLCCALLGAGAALAARSDVTLVVADELRPLLDKH
                     LSVLQGEDEAGLDEVGRIALLRRARKEIGDLLATEGYFSPAVTGEAVTGGNFRILVTP
                     GPRTLVNQVDLRFHGALADPDAERIQRVAALRAAWSLPAGAPFRQADWDAAKQRLLDA
                     VSVRDFAAATITTSHARIDPEAATAALTVEVDSGPPFVFGALQVSGLQDYAEDLVRRF
                     NPPAPGEAYSLDRLLRFQSALQNTAYFASVIVDVDRQSDTPGAVPVRVQLTEAKPKRL
                     GFGAGVSSNTGYRVETSYRDANVFDRALSLVSGVRVEERRQLGYADVFLPPSSDGHLD
                     SVGALAEHTNIAGLQTQRQSIGVVRAMPRGNIETRVAFNYQHEILSPEGSLRSSRDAL
                     TANWSWTRRKVDNLLDPRNGHVLNVQVGGATQAFLSDRNFARFYGRFQGFVPVARRDV
                     LILRGEGGITVANSRDGVPQDFLFRTGGAQSVRGYAYQSLGVKDGSATVGGRYLAVLS
                     SEYVHWYEGDWGVAAFFDAGNANDERQLFKMNYGYGLGPRWKSPAGPIALDLAYGQSD
                     HRVRLHFAVAIAF"
     gene            3740..4054
                     /locus_tag="PLH85_16450"
     CDS             3740..4054
                     /locus_tag="PLH85_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB66210.1"
                     /translation="MKKLVVVAAVAGLMGSTAAFADAGMDLAKAKNCLACHAVDKKVV
                     GPAYKEVAAKYKGDKTAEAKLVEKVQKGGSGVWGSMPMPPNPQVSPAEAKTLVAWVLS
                     QK"
BASE COUNT          635 a         1468 c         1524 g          722 t
ORIGIN      
        1 cacgccggcc gcgctgcgcg ccctgtgcct ggagcgcgct gcccaggcgc tggaggcccg
       61 cagccatgcc ctcgtggcgc tcctcgtgcg cgaggccggc aagaccttcg ccgccgggct
      121 cggcgaagtc cgcgaggcgg tagacttctg ccgctactac gcggcccgcc tgcgcgccgg
      181 cgatctgatc ggcagcgcgc ctctggggcc ggtcgtggcc atcagcccgt ggaactttcc
      241 gctggcgatc ttctgcggcc agatcgtggc ggccctggcc gccggcaatc cggtgatcgc
      301 caaaccggcc gagcagacgc cgctgatcgc cgaccaggcc gtggcgctgc tccacgaagc
      361 gggcatcccg cgtggcgtgc tgcaatgcct gcccgggccg ggcgcgaccg taggggctgc
      421 gctggtggcg gatgcccgcg tggcggcggt gatgttcacc ggctccacag aggtggccac
      481 cgccatccac cgggtgctgg ccgatcgcgg cgacgtgccc ctgatcgccg agaccggggg
      541 gctcaacgcg atgatcgtgg attccaccgc gctgccggag caggtggtga cggacgtgat
      601 cgcgtcggcc ttcgattccg ccggtcagcg ctgttcggcg ctgcgtctgc tgtgcctgca
      661 ggacgaaatc gccccgcggg tgctggaact cctcgacggc gccctgggtg aattgcgcat
      721 cggtccgccg gaggacttcg ccaccgacat cggcccggtc atcgacgccg cggcccacct
      781 tcagctggat gcctacgtgg atgccctgcg ggcggacggg cactgcgtca tgacccgcag
      841 cctgcccgac gtctgcaccg acggacattt tgtgatgccc accgtcatcg agctgcgcac
      901 gcccaccgaa cttcaacgcg aagtctttgg tcccgtgctg catgtcgtcc ggttcgccct
      961 gcacgatctg gacgcactgc tcgatgccct cgggcgcagc ggctacgggc tcacgcgcgg
     1021 catccagtcg cgggttgaca gcactgtcga acgcattctc gggcacggcg gcgtcggcaa
     1081 cgtctacgtg aatcgcaaca tgatcggcgc cgtggtgggc gtgcaagcct ttggcggcga
     1141 gggcctctcg ggcaccggac ccaaggccgg cggccccctg ttgctgcggc gcctggtgcg
     1201 gcgggcgccc ggcctgtctc tgcccggtca gtgcgcgcca gcggacctcg acaccctgga
     1261 cgcatggttg accgcggggg ccgggggcgt cgtggacgat gcggcccgcg cccggctgcg
     1321 ggtccgtgtg gcagcctgcc gggccgccac cctgtggggc gccgtgctgg atctgcctgg
     1381 gccgaccggt gagcgcaata cctggcgcgt ccatccccgc gggatggtgc tgggcctggc
     1441 cgaagcggtc gaggacgccg tgcaccaact ggcggcggcc ctggcgaccg gcaaccgcct
     1501 cgccgttccc gcggccgctg cgggctggct gtctgccctg cctgaggcgc tgcggggctg
     1561 cgtggcgctt cgtccgtccg atgggttcga accctggcag gcggcgctgc tcgcgggcgc
     1621 cgagggccgg gtggcggcct ggcgtcggca gctggccgcc cagcccggtc cgctccgccc
     1681 gatcctggtt gctgatccag actacgtcct ggaacgcctc ctggtggagc ggtgcgtcac
     1741 cgtcaacacc gcggcgaccg gcggcaatgc cgggctgatg gggctggggg aaagctgaat
     1801 ccgtcgcgcc atgcggtgct ccggagcgga taggccttag aatccgcccg tgccgtatcg
     1861 agcctgccta cgcctgtcga cgctctgttg tgccctgctg ggggcgggtg ccgcgctggc
     1921 ggcgcggagc gatgtcaccc tcgtcgtggc agacgagctc cggccgctgc tcgacaagca
     1981 tctgagcgtc ctgcagggcg aggacgaggc ggggctcgat gaagtcggcc ggatcgcgct
     2041 gctgcgccgg gcccgcaagg agattggcga tctgctggca acggaaggct acttttcacc
     2101 ggctgtcacc ggcgaggcgg tgacgggggg caacttccgc atcctcgtca cgcctggacc
     2161 gcgcaccctg gtcaaccagg tggatctacg ttttcacggc gccctggccg atccggacgc
     2221 cgagcggatc cagcgggtgg cggcgctacg ggcggcctgg agcctgccgg cgggcgcgcc
     2281 gtttcgccag gcggactggg atgccgccaa gcagcgtctc ctcgacgcgg tgtccgtccg
     2341 cgactttgcg gcagccacga tcaccaccag ccacgcccgt atcgacccgg aggcggcgac
     2401 ggccgccctg accgtcgagg tcgattccgg cccgcccttc gtgtttggcg ccttgcaggt
     2461 gagcggtctg caggattacg ccgaggacct cgtccggcgc ttcaaccccc cggcccccgg
     2521 cgaagcctac agcctcgacc ggctgctgcg cttccagagc gcattgcaga acaccgccta
     2581 ctttgcgtcc gtgatcgtgg acgtggatcg gcagagcgac acgccggggg cggtgccggt
     2641 gcgcgtacaa ctgaccgaag ccaagcccaa gcgcctgggt ttcggtgccg gtgtcagcag
     2701 caataccggc tatcgtgtcg agacgagcta ccgggacgcg aatgtgttcg accgtgccct
     2761 gagcctcgtc agcggggtgc gcgtggagga gcgtcgccag ctcggttacg ccgacgtgtt
     2821 tctgccgccg tcgtccgatg ggcacctgga cagcgtcggg gcgctggccg agcacacgaa
     2881 catcgcgggt ctgcagaccc agcgccagtc gattggcgtg gtgcgggcca tgccccgcgg
     2941 caacatcgag acacgcgtcg ccttcaatta ccagcacgaa atcctgtcgc ccgagggcag
     3001 cctgcgcagc agccgggacg cgctgacggc caactggtcg tggacccgcc gcaaggtgga
     3061 caacctgctc gatccgcgga atggtcacgt gctcaacgtg caggtgggcg gagcgaccca
     3121 ggcgtttctg tccgatcgaa actttgcccg tttctatggc cgcttccagg gcttcgtgcc
     3181 ggtcgcgcgg cgggatgtgc tgatcctgcg cggggagggc ggcatcaccg tggccaattc
     3241 gcgggacggg gtgccccagg atttcctgtt ccgcaccggc ggcgcccaga gcgtacgggg
     3301 ctatgcgtac cagagcctgg gcgtaaagga cggcagcgcc accgtaggcg ggcgctacct
     3361 cgccgtgctc agttccgagt atgtccattg gtacgaaggc gactggggcg tggcggcgtt
     3421 cttcgacgcg ggcaacgcca acgatgagcg ccagctcttc aagatgaact acggctatgg
     3481 cctcggtccg cgctggaaaa gtcccgccgg tcccattgcg ctcgatctgg cctatggtca
     3541 gagcgatcat cgagtccgcc tgcatttcgc ggtcgccatt gcgttctgag cggacgtgcg
     3601 ccggggtggg gcgtcaacgc cacgacccgc ccggacgttg aggggaggcc ggccggcctg
     3661 tgcaggacaa ttggcaatcc ggcgggatga ggattagact cctctgatac gttttttcgc
     3721 caccaccgag aggaatgcaa tgaagaagct cgtagttgtt gccgccgttg ccggtctgat
     3781 gggttccacc gcagccttcg ccgatgccgg catggatctg gccaaggcca agaactgtct
     3841 ggcctgccac gccgtggaca agaaggttgt cggccccgcc tacaaggaag tggctgccaa
     3901 gtacaagggc gacaagaccg ccgaagccaa gctggtcgaa aaggtccaga agggcggctc
     3961 cggtgtgtgg ggcagcatgc ccatgccgcc gaacccgcag gtcagcccgg ccgaagccaa
     4021 gacgctggtc gcctgggtgc tgagccagaa gtaagcggtc tttcggccac acagaagcca
     4081 gccctcgggc tggcttctgt tttttgtgca gggggacccg tgggcgggga tgcctgccat
     4141 tcggcctgtt gcgcagttgc aacactcgat gcggcaagcg ctgcctaaaa acaacgctgt
     4201 ccttgcccaa ccctttccct cccccacaca atgcagtcca acttctcgtt agagaggtga
     4261 aattggtcgg ccccgctgcg gcggtgcgtg ccagtcttca atcaagagga gaaacatatg
     4321 caaaagaaac tgatcgctct ggccgtcgc
//
LOCUS       DAOQMM010000161         4247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_13199_length_4247_cov_52.584924, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000161 DAOQMM010000000
VERSION     DAOQMM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13199_length_4247_cov_52.584924"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLH85_16455"
     CDS             complement(<1..486)
                     /locus_tag="PLH85_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB66211.1"
                     /translation="MSETQAQTAKAPDRPRKKAGNDTEETLYAVRQKVYPRAVTGTFA
                     AWRWTLVWLTQILFYGLCWVPWNGRQAVLLDLVNRKFYIFGWIFWPQDVFFLAVLLII
                     CAYSLFFFTAVAGRLWCGYSCPQTVYTEIFMWIERKIEGDRTQRMKLDQAPMSARKLG
                     LK"
     gene            complement(726..1214)
                     /locus_tag="PLH85_16460"
     CDS             complement(726..1214)
                     /locus_tag="PLH85_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB66212.1"
                     /translation="MALYLSAHEAWERMQQAPAKVLFLDVRTRAEAMYVGMPTAVDAL
                     VPYMEHQEIMSDWDERRHMYLLLPNSDFVQEAVARLQAKGLTRSDPVILICRSGDRSA
                     RAADLLAGAGFTQVYSVVEGFEGDLAKSGPRVGQRVVNGWKNADLPWSYQLDKAKMYF
                     PR"
     gene            1378..1677
                     /locus_tag="PLH85_16465"
     CDS             1378..1677
                     /locus_tag="PLH85_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB66213.1"
                     /translation="MWKIRLTCAIACLGAALAQAADPVLGEKLAREHRCADCHQRLIG
                     GDGSALYTRPDRKVTSRDKLTAQVSFCSSQLKTGWFPEEEEAVAAWLNQRYYHFR"
     gene            complement(1684..2157)
                     /locus_tag="PLH85_16470"
     CDS             complement(1684..2157)
                     /locus_tag="PLH85_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66214.1"
                     /translation="MKSLLRIFAAILLGVVVPAVAGKGSAALSSDLHEPDARRDDLLY
                     VVLFSLPECPYCVAVRQAYLPALPEDPRFRERVRIREVRIGDPRTLSGFDGAPVSHAA
                     LAKRYGVKFAPTVLFLDAAGQPRAEPLLGGDSVGLYGGYLEKRVMQGLGLLPRPD"
     gene            complement(2154..3116)
                     /locus_tag="PLH85_16475"
     CDS             complement(2154..3116)
                     /locus_tag="PLH85_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB66215.1"
                     /translation="MTRFRRTLPPALRVIVALTLACLNGWAAAMDLVPLRVSEHVWYV
                     RGDSGVASAANQGFNSNAGFVVTADSVVVVDALGTPALGEALLTAIRRISTLPVRRVV
                     LTHFHADHFYGLPAFKAVGAEVWAHRAGRAYFASGEAEARLAQRRRDLFPWVDEHTTL
                     IQADRWLDGPVRFSDGGLHFRIDILGPAHSPEDLTLTVEEDGVVFCGDILFAGRIPFV
                     GNADSRRWLAAIDAVAALHPRILVTGHGPHSSDAAADLSLTGDYLRFLREKMGRAVAD
                     MVPFDEAYAQTDWGRFRALPAFDAANRINAYGTYLTLERESLGQ"
     gene            complement(3128..>4247)
                     /gene="soxB"
                     /locus_tag="PLH85_16480"
     CDS             complement(3128..>4247)
                     /gene="soxB"
                     /locus_tag="PLH85_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HNB66216.1"
                     /translation="NIKTADFGDPVFKPYVIRPINGVPVAIVGQAFPYTPIANPRWQV
                     PNWRYGIQDDALQVVVDEARAAGAQVVVVLSHNGMDVDLKMAGRVRGVDAILGGHTHD
                     GVPAPVIVPNAGGRTLVTNAGSNGKYLGVLDLEVRDGRVTDFRYKLLPVFANLIPADP
                     AMAALIDRLRAPYAARLAEPLAVTEALLYRRGNFNGSWDQLILDALLAEKNAEIAFSP
                     GFRWGTSLLPGDTLRMEHLLDQTAITYPWTTLTSMSGAQIKTVLEDVCDNLFNPDPYY
                     QQGGDMVRVGGLQYTCDPNARMGSRILDMRLHGQPLQADKQYKVAGWAPVSEEARDTG
                     GEPVWDLVARYLKGQKVLRARPLNLPTLRGMAGNPGIA"
BASE COUNT          716 a         1421 c         1463 g          647 t
ORIGIN      
        1 cttgagtccg agcttgcggg cgctcatggg cgcctggtcg agcttcatgc gctgggtccg
       61 gtcaccctcg atcttgcgct cgatccacat gaagatctcg gtgtagaccg tctgcggaca
      121 gctatagccg caccacaatc gcccggcgac cgcggtgaag aagaacaggg aatacgcgca
      181 gatgatcagc agcacagcca ggaagaacac gtcctggggc cagaagatcc agccgaagat
      241 gtagaacttg cgattgacca gatccagcag caccgcctgc cgcccgttcc agggcaccca
      301 gcagaggccg tagaagagga tctgggtgag ccacaccagc gtccaccgcc acgcggcgaa
      361 ggtgccggtc acggcgcggg gatagacttt ctggcggacg gcgtagaggg tctcttcggt
      421 gtcgttcccg gctttcttgc gggggcgatc cggggctttc gcggtttggg cttgggtttc
      481 gctcatggct gatgacctgc aagggaaatc ggatcgtcgt ccggtactgg acgacctgtc
      541 agcccaggtc caatcaatat ccgtaccagc caccccgcag acggatcatt tgtttgatat
      601 caaaggcttg cctgaaattc ccgcaatccc gccagcgcct ttcgatctcg aaacggcagt
      661 ccggactgcc acgatctgcc aggaccgaca gcactgtcag tcctgccagg gctgctgacg
      721 atccctcagc gcggaaagta catcttcgcc ttgtccagct ggtaggacca cggcaagtct
      781 gcgttcttcc agccgttcac gacgcgctgc ccgacccgcg ggccggattt cgccagatcg
      841 ccctcgaagc cctcgacgac gctgtacacc tgggtgaagc cggccccggc cagcaggtcc
      901 gccgcccgtg cgctgcggtc gccggaccgg cagatgagga tcaccggatc gctccgggtg
      961 aggcctttgg cctgcaggcg ggcgaccgcc tcctggacga agtcgctgtt ggggagcagc
     1021 aggtacatgt gacggcgctc gtcccagtcg gacatgatct cctggtgctc catgtacggc
     1081 accagcgcgt caacggccgt cggcatgccc acgtacatgg cctcggcccg cgtccgcacg
     1141 tccaggaaga gcaccttggc cggcgcctgc tgcatccgct cccaggcctc atgagcgctc
     1201 aggtagaggg ccaaacgggt ccgtttcgct tccggcagcg tggcgggatc gaccgcagct
     1261 gctatcgtgg aaaggggcgc caggagcagc cccagagtgc agatacacgc caacagccgt
     1321 ttgctcacga ttcgtccttt cgaggggatg gatcggccca gtccggagac cctgccgatg
     1381 tggaaaatcc gtctgacctg cgccatcgcc tgcctgggcg ccgccctggc ccaggccgcc
     1441 gacccggtcc tgggagaaaa gctcgcccgc gagcatcgct gcgcggactg ccaccagcgc
     1501 ctcatcggcg gcgacggctc ggccctgtac acgcggccgg accgcaaggt caccagccgc
     1561 gacaaactca ccgcccaggt cagcttctgc agcagccagc tcaagaccgg ctggttccct
     1621 gaggaggagg aagcggtcgc agcctggctg aaccagcggt attaccactt caggtaatct
     1681 cccctaatcc ggccgcggca gcaggcccag gccctgcatc acacgctttt ccagatagcc
     1741 gccgtacagg cccacactgt cgccccccag caggggctcg gcgcggggct ggccggccgc
     1801 gtcgaggaac agcaccgtgg gtgcaaactt cacgccatag cgcttcgcca gcgctgcatg
     1861 actcaccggc gctccgtcga agccgctcag cgtgcgcgga tcgccgatgc gcacctcccg
     1921 gatgcgcacc cgctcacgga agcgggggtc ctccggcagg gccggcagat acgcctgtcg
     1981 cacggcaacg cagtacgggc actccggcag gctgaagagc acgacgtaga gcagatcgtc
     2041 gcgccgggcg tcgggctcgt gcagatcgga cgacagggcc gcactgcctt tgcccgccac
     2101 cgccggcacc accaccccga ggagaatcgc ggcgaagatc cgcagcagcg acttcactgg
     2161 ccgagggact cccgttcgag ggtcaggtag gtgccgtaag cgttgatgcg attggccgca
     2221 tcgaaggccg gcagcgcccg gaagcggccc cagtccgtct gcgcgtaggc ctcgtcgaag
     2281 ggcaccatgt ccgccacggc gcggcccatc ttctcccgca ggaagcgcag gtagtcgccg
     2341 gtgaggctca ggtccgccgc cgcatcgctc gaatggggcc cgtggccggt aaccaggatg
     2401 cgcggatgca gggccgcaac ggcgtcgatg gccgccagcc agcgccggct gtcggcgttg
     2461 ccgacgaagg gaatgcgccc ggcgaagagg atgtcgccgc agaacaccac gccgtcctcc
     2521 tcgacggtca gggtcagatc ctcgggggaa tgggccggtc ccaggatgtc gatgcggaag
     2581 tgcagcccgc cgtcgctgaa gcgcaccggg ccgtccagcc agcgatcggc ctggatcagc
     2641 gtggtgtgct cgtccaccca cggaaagagg tcgcgccggc gctgggccag acgcgcctcc
     2701 gcctcaccgc tggcgaaata cgcgcgtccg gcacggtggg cccacacctc ggcgccgacc
     2761 gccttgaagg ccggcaggcc gtaaaaatga tcggcgtgga agtgggtcag cacgacccgt
     2821 cgcaccggca gcgtggagat gcggcggatc gccgtgagca gggcctcgcc cagggccggc
     2881 gtacccaggg catccacgac gacgacgctg tcggcggtga ccacgaagcc ggcgttgctg
     2941 ttgaagccct ggttggccgc cgaggccacg ccggaatcgc cgcgcacgta ccacacgtgc
     3001 tcggagacac gcagcggcac gaggtccatg gccgcggccc agccgttcag acacgccagg
     3061 gtgagggcga cgatgacccg cagcgcggga gggagggtgc gccggaagcg ggtcatcgtc
     3121 ggacgcctca agcgatgcca ggattgccgg ccatgccccg cagtgtgggc aggttgaggg
     3181 ggcgcgcgcg cagcaccttc tggcccttga ggtaacgggc caccagatcc cacacgggtt
     3241 cgccgccggt gtcgcgggcc tcttcggaca cgggcgccca gccggccacc ttgtactgct
     3301 tgtcggcctg caggggctgg ccgtgcaggc gcatgtcgag gatgcggctg cccatgcggg
     3361 cgttgggatc gcaggtgtat tgcaggccgc ccacacgcac catgtcgccg ccctgctggt
     3421 agtaggggtc cgggttgaag aggttgtcgc agacgtcttc cagcacggtc ttgatctgcg
     3481 cgccgctcat cgacgtgagc gtggtccacg ggtaggtgat ggcggtctgg tccagcaggt
     3541 gctccatgcg cagggtgtcg ccaggcagca gcgaggtccc ccagcggaaa cccggtgaga
     3601 aggcgatctc ggcgtttttc tcggcaagca gggcgtcgag gatgagctgg tcccaggagc
     3661 cgttgaagtt gccgcggcgg tacagcagcg cctcggtgac ggccagaggc tcggcgagcc
     3721 gggccgcgta gggcgcgcgc agccggtcga tgagcgcggc catggccggg tcggcgggga
     3781 tcaggttggc gaagacgggc agcagcttgt agcggaaatc cgtgacgcgg ccgtccctca
     3841 cctccaggtc gagcacgccc aggtacttgc cgttggagcc ggcgttggtg accagcgtcc
     3901 ggccacccgc gttcggcacg attaccggcg cgggcacgcc gtcgtgggtg tggccgccga
     3961 ggatggcatc cacgccacgc acccggccgg ccatcttgag atccacgtcc atgccgttgt
     4021 gggacagcac caccaccacc tgggcgccgg ccgcgcgggc ttcgtccacg acgacctgca
     4081 gggcatcgtc ctggatgccg tagcgccagt tgggcacctg ccagcggggg ttggcgatgg
     4141 gggtgtaggg gaaggcctgg ccaacgatgg ccacgggcac gccgttgatg gggcggatca
     4201 cgtagggctt gaacaccggg tcgccgaagt cggcggtctt gatgttc
//
LOCUS       DAOQMM010000162         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_13683_length_4152_cov_41.924579, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000162 DAOQMM010000000
VERSION     DAOQMM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13683_length_4152_cov_41.924579"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLH85_16485"
     CDS             <1..289
                     /locus_tag="PLH85_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66217.1"
                     /translation="DYHPTNPDYSLEAVAALLQRMLDAEEAEVQAQATLSAARDATVA
                     AQWAFHTSVLGVKGQIKALYGEDSDQVATIGLKKKSDRKSPSKSTKPPTTT"
     gene            461..742
                     /locus_tag="PLH85_16490"
     CDS             461..742
                     /locus_tag="PLH85_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66218.1"
                     /translation="MKNTTAIFALLLAGCASTGVVPVAHDEYTISKQSAAGMLGSPNG
                     VKADIYDEAHAFCTQQGKDVETVALDLKHAIPFVQPGSASLKFKCVAKS"
     gene            complement(885..1490)
                     /locus_tag="PLH85_16495"
     CDS             complement(885..1490)
                     /locus_tag="PLH85_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNB66219.1"
                     /translation="MTPGSCSTGIHILLEEVGAVFEVYIVNLPAGDHLKADYVALNPK
                     STIPVLVRKDGTALTEFQAIAWWLARQYPKRSLLPDDAEGEVRVLEVMDYVVGTIHGQ
                     GFTRIFTTPNFTPSPADHPVVQARGREIVEQGFAVLDATLAGRDYVTGRFSIADAALF
                     YTEFWADRLKMDLPPNCRAHYQRMLERPAVRQVLREEGYRV"
     gene            complement(1690..2769)
                     /locus_tag="PLH85_16500"
     CDS             complement(1690..2769)
                     /locus_tag="PLH85_16500"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNB66220.1"
                     /translation="MPTIKVMIVDDSAVVRQVLSSTLDEDAAIEVIGTAVDPVFALER
                     MKSRWPDVIVLDVEMPRMDGITFLRKIMAERPTPVVICSTLTEKGSETALQALSAGAV
                     CVVTKPKLGLKQFLQDSSDDLVSAVKAASKANVRRLAAAARPAPPPAPPVHSADVILP
                     AGQAMVQTTETVIAIGTSTGGTQALEAVLRSLPRMSPGIVVVQHMPEKFTEAFAQRLD
                     SLCEVDVREARHGDRVVPGRVLIAPGGRHMLLRRSGAQYVVDVVDGPPVSRHRPSVDV
                     LFRSTARFAGRNAVGIIMTGMGDDGARGLKEMHEAGAWTVAQDEATCVVYGMPKEAVR
                     LGAVDKVLGLHDIPAAILGRISRKG"
     gene            complement(2786..3289)
                     /locus_tag="PLH85_16505"
     CDS             complement(2786..3289)
                     /locus_tag="PLH85_16505"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNB66221.1"
                     /translation="MRKPDGVVEIFLQPGEWYFGDRYTRIRTVLGSCVSAVFWHSGLL
                     MGGMCHFVLPSRGRLPAAADLDGRYGDEAFRLMVRELRGSGGRPDDFQVRLFGGANMF
                     PAIARGSRGPLVGQKNVDAARALVRDHGLKCVGAHVEGEGHRHLLFDVWSGRVTLKGA
                     HGDTSSP"
     gene            complement(3294..4139)
                     /locus_tag="PLH85_16510"
     CDS             complement(3294..4139)
                     /locus_tag="PLH85_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNB66222.1"
                     /translation="MTAPASHASAPTAGGPGPLAEREFHLFRTLMHRHAGISLSDAKR
                     QLVAGRLAKRLRQLELDSYASYFRLLERNTAERQTAVDLLTTNETYFFREPKHFDFLR
                     EHILPELKGHSPLRVWSAACSSGEEPFTLAMVMAETLGNRPWEIVASDLSTRVLEAAR
                     EGRYTLADAEGIPRPLLVRYGLRGVGSQEGSFLFKPDLRKRIQFRQINLNTVLPDLGL
                     FDVIFLRNVMIYFDMDTKVQVVKRVLAQLRPGGYLFLSHSENLNGVSDAVKSVRPSVY
                     RKPEA"
BASE COUNT          635 a         1338 c         1555 g          624 t
ORIGIN      
        1 cgactaccac cccaccaacc cggactacag cctggaggcc gtcgcggcgc ttctccagcg
       61 catgctcgac gcggaggagg ccgaggtgca ggcccaggcg accttgtccg ccgcccgcga
      121 tgccacggtg gcggcccagt gggccttcca cacgagcgtg ctcggggtga agggccagat
      181 caaggcgctc tacggggagg attccgacca ggtggcgacc atcggcctca agaagaagtc
      241 cgaccgcaag tcgccgagca agagcaccaa gccgcccacg acgacctgag ggcatcacgg
      301 cgggacgggg gccgggcaca tccgcgcggg tggcccatcc gccccgctca caccggcgcc
      361 cccgcccaga acactcgatt ccgccaggaa cgcaagggta ttccgcaaca ccgtgccgat
      421 gggacagggc acggtctaag ccgcaccatc aaggaccaac atgaagaaca cgaccgccat
      481 tttcgcgctg ctgctggcag gctgcgcatc gaccggggtg gtcccggtcg cccacgatga
      541 atacacgatc tcgaagcaga gtgccgccgg catgctgggc tccccgaacg gggtcaaggc
      601 cgacatctat gacgaggccc atgcgttctg cacgcagcag ggcaaggacg tcgagacggt
      661 cgcgctcgat ctgaagcacg ccattccttt tgtccagccg ggcagcgctt ccctgaagtt
      721 caagtgcgtc gcgaaaagct gatggacggc gtgcactggc gcgcggtgct gcccgcacgg
      781 ggcgccccgc gccagccttg agggcgcacc gtaccggctg cccggccggc gcggccctgg
      841 atgccgtcgg tggcgggacg tctgcccgcc tcgcgccccc cttcctagac ccgatacccc
      901 tcctcccgca gcacctggcg cacggccggg cgttcgagca tgcgctggta gtgggcgcgg
      961 caattcggcg gcaggtccat cttgagacgg tcggcccaga attcggtgta gaacagggcc
     1021 gcgtcggcga tggagaagcg gccggtcacg tagtcccgcc cggccagggt ggcgtcgagc
     1081 acggcgaagc cctgttcgac gatctcgcgg ccgcgggcct gcacgacggg gtggtcggcc
     1141 gggctggggg tgaagttggg cgtggtgaag atgcgggtga agccctggcc gtggatggtg
     1201 ccgaccacgt agtccatgac ttccagcacc cgcacttcgc cttcggcgtc gtcgggcagc
     1261 aagctgcgtt tggggtactg gcgggccagc caccaggcga tggcctggaa ttcggtgagg
     1321 gcggtgccgt ccttgcggac caggacgggg atggtggatt tggggttgag ggccacgtag
     1381 tcggccttga ggtggtcgcc ggcaggcagg ttgacgatgt acacctcgaa gacggcgccc
     1441 acttcctcca gcaggatgtg gatgccggtg gagcaggaac cgggggtcat gtagaacttc
     1501 acgggggtgg cctcggcagg gaagatgccc ctggcaagca agagcgggac cacgcccgcg
     1561 ccgcggcggg cctgtgccgc ccgggccggc gggcgtccgc gctggatgcg ggcggtcgca
     1621 tctgccacag cgcatgtcgg ctttgcgcca cgggcgcgcc gggcgctccg ccccgccggg
     1681 cggcgatctt cagcccttgc ggctgatgcg gccgaggatc gcggcgggga tgtcgtgcag
     1741 gcccagcacc ttgtccacgg cgccgaggcg cacggcctcc ttgggcatgc cgtagacgac
     1801 gcaggtggct tcgtcctggg ccacggtcca cgcgccggcc tcgtgcatct ccttgagccc
     1861 gcgggcgccg tcgtcgccca tgccggtcat gatgatgccc accgcgttgc gcccggcgaa
     1921 gcgggcggtg gagcggaaca gcacgtccac cgaggggcga tggcggctca ccggggggcc
     1981 atccaccacg tccacgacgt actgcgcgcc actgcggcgc aggagcatgt ggcggccgcc
     2041 gggggcgatg aggacgcggc cgggaacgac ccggtcgccg tggcgggctt cgcgcacgtc
     2101 cacctcgcac aggctgtcga ggcgctgggc gaaggcttcg gtgaactttt cgggcatgtg
     2161 ctggaccacc acgatgcccg gcgacatgcg cggcagggac cgcagcacgg cttcgagggc
     2221 ctgggtgccg ccggtggagg tgccgatggc gatcactgtc tcggtggtct ggaccatggc
     2281 ctgaccggcg gggaggatca cgtcggcgga atggacgggc ggcgcgggcg ggggcgccgg
     2341 ccgcgcggcg gcggcgaggc ggcgcacgtt ggccttggac gcggccttga cggcgctcac
     2401 caggtcgtcg gagctgtcct gcaggaactg cttgaggccc agcttgggct tggtgacgac
     2461 gcacacggcg ccggcggaga gggcctgcag ggcggtctcg gagccctttt cggtgagggt
     2521 ggagcagatc acgaccggcg tggggcgctc ggccatgatc ttgcgcagga aggtgatgcc
     2581 gtccatgcgg ggcatctcca cgtcgagcac gatcacgtcg ggccaccggg atttcatgcg
     2641 ttccagggcg aagaccgggt ccacggcggt gccgatcact tcgatggcgg cgtcttcgtc
     2701 cagggtggag gacagcacct ggcggaccac ggcggagtca tccacgatca tcactttgat
     2761 ggtgggcatt gttcttgtct ctctgtcagg ggctggaggt gtcgccgtgg gcgcccttga
     2821 gggtcacccg cccgctccac acgtcgaaga gcaggtggcg gtggccctcc ccttccacgt
     2881 gggcgccgac gcatttgagg ccatggtcac ggaccagggc gcgggcggcg tccacgttct
     2941 tctggcccac caggggcccg cggctgccgc gggcgatggc gggaaacatg ttggcgccgc
     3001 cgaagaggcg cacctgaaag tcgtccgggc gcccgccgct gccgcgcagc tcgcgcacca
     3061 tgaggcggaa ggcttcgtcg ccgtagcggc cgtccaggtc ggcggcggcg ggcaggcggc
     3121 cgcggctggg cagcacgaag tggcacatgc cgcccatcag caggccggaa tgccagaaca
     3181 cggccgagac gcaggagccg agcacggtgc ggatgcgggt gtagcggtcg ccgaaatacc
     3241 attcccccgg ttgcaggaag atctcgacga cgccgtcggg tttgcgcatg gcctcatgcc
     3301 tcgggtttgc ggtaaacgga cggacgcacg ctcttgaccg cgtcgctgac cccgttgagg
     3361 ttttccgagt ggctgaggaa gaggtagccg cccggccgca gctgggccag cacccgcttg
     3421 accacctgga ccttggtgtc catgtcgaaa tagatcatga cgttgcgcag gaagatgacg
     3481 tcgaagaggc ccaggtccgg cagcacggtg ttgaggttga tctggcggaa ctggatgcgc
     3541 ttgcgcagat cgggcttgaa caggaagctg ccttcctggc tgcccacgcc gcgcaggccg
     3601 tagcgcacca gcagcggacg ggggatgccc tcggcgtcgg cgagggtgta gcgcccttcc
     3661 cgcgccgctt ccagcacgcg ggtggagaga tccgaggcga cgatctccca cgggcggttg
     3721 ccgagggtct cggccatcac catggcgagg gtgaagggtt cctcgccgct ggagcaggca
     3781 gcgctccaca cgcggagggg gctgtggccc ttcagttccg gcaggatgtg ctcgcgcagg
     3841 aagtcgaagt gtttgggctc gcggaagaag taggtctcgt tggtggtgag caggtccacc
     3901 gcggtctggc gctccgcggt gttgcgctcc agcaggcgga agtagctggc gtagctgtcc
     3961 agctccagct gacgcaggcg cttggccagg cgaccggcca ccagctgccg cttggcgtcg
     4021 gacaggctga tgccggcgtg gcgatgcatc agggtgcgga acaggtggaa ttcccgttcc
     4081 gccagggggc cggggccccc ggcggtgggt gcggaggcat gcgatgccgg ggcagtcatg
     4141 ggggcgatct cc
//
LOCUS       DAOQMM010000163         3934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_15007_length_3934_cov_54.849961, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000163 DAOQMM010000000
VERSION     DAOQMM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15007_length_3934_cov_54.849961"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..81
                     /locus_tag="PLH85_16515"
     tRNA            5..81
                     /locus_tag="PLH85_16515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Pro,seq:cgg)
     gene            complement(846..1400)
                     /locus_tag="PLH85_16520"
     CDS             complement(846..1400)
                     /locus_tag="PLH85_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNB66223.1"
                     /translation="MSKIAVVFHSGYGHTLRLAQSVAVGAGAELVPIDAEGNLPEGGW
                     DTLNAADAIIFGSPTYMGTVSWQFKKFADASSKAWFTQAWKDKVFAGFTNSATMNGDK
                     LSTLHYLFTLAMQHSGVWVGTGLMPSNSKAAQRDDVNYVGSFCGAMAQTPSDASPDEI
                     PAGDLETARLFGQRVAAVTARFGR"
     gene            complement(1441..2139)
                     /locus_tag="PLH85_16525"
     CDS             complement(1441..2139)
                     /locus_tag="PLH85_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB66224.1"
                     /translation="MLALRRARDRGFADHGWLQSHHTFSFAGYHDPAHMGWGNLRVIN
                     EDRIAPGTGFGAHSHRDMEIVSYVLNGALAHRDSLGNGTVIQPGDVQRMTAGTGVTHS
                     EFNDAAETTHFLQIWLLPARRNLPPGYAQRTFPEADKRGRLARIAAPDGADGAVLLHA
                     DACVYAGLLDGAEQFTLDLDPSRKAFVHLIRGRLRVNGQMLEPGDGAALADENRLHLD
                     AAEDAEVLVFDLAP"
     gene            2305..3195
                     /locus_tag="PLH85_16530"
     CDS             2305..3195
                     /locus_tag="PLH85_16530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB66225.1"
                     /translation="MIQRIAELGSFAAAARATGQVPSALSYRVRQIEEALDVLLFDRS
                     ARQARLTEAGGELLREGARLLGEIDAIANRVKRVATGWEPRLTIAVDSIIHRTTVLEL
                     CEAFFALGAPTRLRLRQETLSGTLETLGSGEADLALGVAVESISVAGVQGRPLGSIRF
                     LFAVAPHHPLARAPEPLSDELIGRHRAVAVADTVRVGSGQTVGLLGGQDVFTVPNLEA
                     KLEAQLRGIGAGFLPECLVRSYADSGRLVTKAVQRSERVVHLGYAWRAAGSGVPRKAL
                     QWWLERLEHAATRAALLGVR"
     gene            complement(3208..3816)
                     /locus_tag="PLH85_16535"
     CDS             complement(3208..3816)
                     /locus_tag="PLH85_16535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB66226.1"
                     /translation="MDEHLIVLDKPSGLLSVPGRGLERADCLASRVHVRFADARIVHR
                     LDMATSGLIVMARGAHMERALSIAFQQRQVEKCYEALVAGRPAQVEGDIHLPLITDWP
                     RRPRQKVDPDTGKPSHTHFRVLSYDPGADVSRVALIPHTGRSHQLRVHLQAIGHPILG
                     DELYAPDAAFQAAPRLMLHACRLCFAHPALGHRLELNSPPPF"
BASE COUNT          656 a         1166 c         1391 g          721 t
ORIGIN      
        1 gtgtcgggga atagctcagc ctggtagagc actgcgttcg ggacgcaggg gccggaggtt
       61 cgaatcctct ttccccgacc atcactttaa aaagccactc tgcggagtgg cttttttgcg
      121 tgctacgcct tccggattta ggttttctag gcatgcatca tgccgacgtc ggatctctgg
      181 gcctcaaccg ttgtcctgtc tcgcgcgatg tcctgcaccg gaagacgcac gaatcgtgag
      241 cggctggagg cgggttggcg ggcacagata ccgcggtgag tgggtgctcg attccagttc
      301 gtttgcgatt aatggcgcgg tatcgtgtcg agtcaggggc gcgctgccac ggctgtctaa
      361 ctcaggggaa caacggcgag ttactcagaa tcgggtgcgc gagctgacga aacctgacgt
      421 cagtgcaaac gtggttgaag ggacgtcccc gtaccgccta ttcaaggctg ttcaggctgc
      481 ctgccatcac cctgacgtac ggcgtgggtt tggtaccgac cgcattccgg acgatcacaa
      541 cgatgcgcaa cctggcggcg agcgtctggc acggcggacc tgggtgatgc tccgggagat
      601 gcaccattgc tccgcctcgg cgcctgcgac cttcctgggc ggatttgtca cgtggggttg
      661 ggccaacggt cgatctgcga tgcgggccga ccttcgtcct gccgccgagg gtgtgcgcgt
      721 gaggattccc ggcaaggcgg ggcggatgcc tcgtaccgat gggtgccgtg acttggtcta
      781 accgatttcc gcccgttgga gggacattgc ggcgggcgat gcgccgcaat gtccgcagag
      841 gtagatcagc gaccgaaacg ggcagtcact gcagcgacgc gctggccgaa caggcgtgcc
      901 gtctccaggt ccccagccgg aatttcgtcg gggctggcgt cggagggggt ctgggccatg
      961 gcgccgcaga aggagcccac gtagttcacg tcgtcccgct gcgcggcctt gctgttggac
     1021 ggcatcaggc cggtgccgac ccacacgccg ctatgctgca tggccagggt aaacaggtag
     1081 tgcagagtag acagcttgtc gccattcatg gtggcactgt tggtgaagcc ggcaaagacc
     1141 ttgtccttcc atgcctgcgt gaaccaggcc ttggagctgg cgtcggcaaa tttcttgaat
     1201 tgccagctca cggtgcccat gtaggtcggg ctgccaaaga tgatggcgtc agctgcgttc
     1261 aatgtgtccc agccgccttc cggcaggttg ccttcggcat cgatgggaac gagctcggcg
     1321 ccggctccga cggcgacgga ctgggcgagg cgcagggtgt ggccatagcc ggaatgaaag
     1381 acgacggcga tcttgctcat gtggttctcc aggggtgggg gtaaagtcgt tggggggcga
     1441 tcagggtgcc agatcgaaga ccagcacttc ggcgtcctcg gcggcgtcca gatgcagccg
     1501 gttttcatcg gcgagcgcgg cgccgtcgcc gggttccagc atctggccat tgactcgcaa
     1561 gcggccgcgg atcagatgga caaaggcctt gcgggacgga tccaggtcca gcgtgaactg
     1621 ctcagcgccg tccagcagcc ccgcatagac gcaggcgtcg gcatgcagga ggacggcacc
     1681 gtctgcaccg tcgggcgccg caatccgcgc cagccggccg cgtttgtcgg cctctggaaa
     1741 agtacgttgc gcgtacccag gcggcagatt gcgccgggcg ggaagcagcc agatctgcaa
     1801 gaaatgggtg gtctccgcag catcgttgaa ttcgctatgg gtgaccccgg tgcctgccgt
     1861 catgcgctgt acgtcgccgg gctggatcac cgtgccattg cccagactgt cgcgatgggc
     1921 gagggcgccg ttgagtacgt agctcacgat ctccatgtcc cggtggctgt gggcgccaaa
     1981 gccggtaccc ggcgcgatgc ggtcctcatt gatgacacgc aggttgcccc agcccatgtg
     2041 agcagggtcg tggtaaccgg caaaggagaa ggtgtggtgg ctttgcagcc agccatggtc
     2101 tgcgaaaccc cggtctcggg cgcggcggag tgcgagcatg atgggcctcc tggtgtctgg
     2161 gtgaagtcac tttaccgggg tcatcggggc gcatgagcca cggcgtttga tggcatcatt
     2221 caaattattc gaatgatttg tgtttttact atgacttcca gtgcactgac tccccgggac
     2281 gtcctgacgc ccgaggcatt ggcgatgatc cagcgcatcg ccgaactggg cagtttcgcc
     2341 gccgcggcgc gggcgaccgg acaggtgccg agcgccttga gctatcgcgt gcgccagatc
     2401 gaagaggcgc tggatgtgct gctcttcgac cgcagcgcgc gccaggcccg actgaccgag
     2461 gctggaggcg aactgttacg ggagggcgcc cggctgctgg gtgagatcga tgccatcgcc
     2521 aatcgggtca aacgggtggc caccggttgg gaaccccgcc tcaccatcgc ggtggacagc
     2581 atcattcacc ggacgaccgt gctggagctg tgcgaggcct tctttgccct gggcgcaccg
     2641 acgcgcctgc ggcttcgtca ggagaccctc tccggcacac tggagacgct gggctcgggc
     2701 gaggccgatc tggccctcgg tgtagcggtg gagtcgatct cggtggcggg cgtacaggga
     2761 cgtccgctgg gatccatacg cttcctgttc gcggtggcac cccaccaccc actcgcccgc
     2821 gcacccgagc ccctcagcga tgagctcatt ggccgccatc gtgccgtggc cgtcgcggac
     2881 accgtacgtg tcggcagcgg tcagactgtg ggactgctgg gcgggcagga cgtgttcacc
     2941 gtgccgaacc tggaggccaa gctggaagct caattgcgcg gtatcggagc gggattcctg
     3001 ccggaatgcc tggtccggtc ctacgctgac agcggccgtc tggtgaccaa ggcagtccag
     3061 cgcagcgaac gcgtggtaca tcttggctac gcatggcgag cagccgggtc cggagtgccg
     3121 cgcaaggcgc tgcaatggtg gctggagcgc ctcgagcacg cggcgacccg ggcggcgctg
     3181 ctgggcgtgc gttgaggacg cagtccgtca gaaggggggc gggctgttca gctccagccg
     3241 gtgcccgagg gctggatggg cgaaacacag gcggcatgcg tggagcatca gccggggggc
     3301 cgcctggaag gcggcatcgg gagcgtagag ttcgtcgccg aggatcgggt ggccgattgc
     3361 ctgtagatgg acgcgcaact ggtgggagcg ccccgtgtgc gggatgaggg ccacgcggct
     3421 cacgtcagcg cccggatcgt aagagagcac gcggaagtgg gtgtgactgg gcttccccgt
     3481 gtcaggatcc accttctggc ggggtcgccg gggccagtcg gtgatcaagg gcaggtggat
     3541 gtccccttcg acctgggcag ggcgtcctgc cacgagcgct tcgtaacatt tctccacctg
     3601 ccgttgctgg aacgcgatgc tgagggcgcg ttccatgtgg gcacccctgg ccatgacgat
     3661 cagcccggag gtggccatgt ctagccgatg gacgatgcgt gcatcggcaa accggacgtg
     3721 tacccggctc gccaggcaat ccgcacgctc gagcccgcgt cccggcaccg acagcagtcc
     3781 cgaaggtttg tcgagcacga tgagatgctc gtccatgaag acgacgatgg gttcgcctcg
     3841 aggcggcagg taagggggcg cgggcggcgt tgcggtggat tccgcggcca taccgatcag
     3901 atgattgaag cgttgaaaaa acgagaccgt cact
//
LOCUS       DAOQMM010000164         3864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_15491_length_3864_cov_56.507220, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000164 DAOQMM010000000
VERSION     DAOQMM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15491_length_3864_cov_56.507220"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLH85_16540"
     CDS             complement(<1..251)
                     /locus_tag="PLH85_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HNB66227.1"
                     /translation="MSTMTREETDALIAEVLEVYPDKAKKDRKKHLASNDVTVEQSKK
                     CITSNRKSLPGVMTIRGCAYAGSKGVVWGPIKDMIHISHG"
     gene            complement(343..1224)
                     /gene="nifH"
                     /locus_tag="PLH85_16545"
     CDS             complement(343..1224)
                     /gene="nifH"
                     /locus_tag="PLH85_16545"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HNB66228.1"
                     /translation="MAKLRQCAIYGKGGIGKSTTTQNLVAALAEAGKKVMIVGCDPKA
                     DSTRLILHSKAQTTVMHLAAEAGSVEDLELEDVLSVGFGGVKCVESGGPEPGVGCAGR
                     GVITAINFLEEEGAYDEDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAM
                     YAANNISKGIVKYANSGGVRLGGLICNSRNTDREDELIIALAARLGTQMIHFVPRDNA
                     VQHAEIRRMTVIEYDPKHKQADEYRQLAMKVMNNTKLVIPTPIEMEELEDLLMEFGIM
                     EAEDESIVGKTAADLAA"
     gene            2006..2842
                     /locus_tag="PLH85_16550"
     CDS             2006..2842
                     /locus_tag="PLH85_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNB66229.1"
                     /translation="MTPELRATLLAGIQDGSIVPYLGAGVLADVKNVATGEPIPATSD
                     ALIIAMNGGKPMAPKLMYEFPRAAMNVELKRGRSTVNKFLTRTYGETQWTRGAVHDWI
                     KAIRPRYVIDINRDTQLQDSYADVPHNLIVGIARIGGTTLRYKIFFWDGSSYQRAEEL
                     KLEHPILFKPVGTPRPEPTYIASDADYVDFITELMGGFSIPPQLKEFRKGKQYLLAGL
                     RLNRDTERMVMADIIYSAAEPAGWVFIAEPTDKERRYCKKIGLEIVDADIFDLIGEPQ
                     PA"
     gene            2871..3428
                     /locus_tag="PLH85_16555"
     CDS             2871..3428
                     /locus_tag="PLH85_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66230.1"
                     /translation="MLLDRYEQYFFNANFREAADEAGWEAYRGELVLKEGEIADDKGR
                     RKPPHEVLKQVVVLAKGDELKLISGHLDALQHIPGFVEKYAADLKADTIAVLFAVNID
                     DPFLAKIGEGTVAFIPLVQGMAWNELIDLVALEKGDFKGQSAAEKVVTVFNGFKGHKF
                     KYPETTLDDALKSTNNAKREMHGAV"
     gene            3428..3811
                     /locus_tag="PLH85_16560"
     CDS             3428..3811
                     /locus_tag="PLH85_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNB66231.1"
                     /translation="MPWDDAQSLGVADMDATHREFVEHVDALASADDSAFPALFLSLQ
                     EHTRLHFEHEGKLMKACRFPAIGEHNSEHLRVLGEFAHFARGVNAGRLTPARAYIQAL
                     PGWFATHLATMDAALAGCLKRSRGE"
BASE COUNT          723 a         1243 c         1187 g          711 t
ORIGIN      
        1 ccgtgggaga tgtggatcat gtccttgatc ggaccccaca ccacaccctt ggaaccggcg
       61 taggcacagc cgcggatggt catcacgccg ggcagggact tgcggttgga cgtgatgcac
      121 ttcttggatt gttcgacggt gacgtcgttg gacgccaggt gcttcttgcg gtccttcttg
      181 gccttgtcgg gataaacctc aagcacctcg gcaatgaggg catcggtttc ttcgcgagtc
      241 atggtgctca tattttcctc ctgacgccgc caccaatctg tggcagacgt gctggttgaa
      301 agcagcgggt accggagtac ccgccgtgca gcagatgctt gattaggcgg ccagatcggc
      361 ggcggtcttg ccgacgatgg actcgtcttc cgcttccatg atgccgaatt ccatcagcag
      421 gtcttccagc tcttccatct cgatcggggt cgggatgacc agcttggtgt tgttcatgac
      481 cttcatggcc agctgacggt attcgtcggc ctgcttgtgc ttcggatcgt attcaatgac
      541 ggtcatacga cggatttcag cgtgctgcac cgcgttgtca cggggcacga agtggatcat
      601 ctgggtgccg aggcgggcgg ccagggcgat gatcagttca tcttcgcggt cggtgttgcg
      661 ggagttgcag atcaggccgc ccagacgcac gccgccggag ttggcgtact tcacgatgcc
      721 cttggagatg ttgttggccg cgtacatggc catcatttcg ccggagcaga cgatgtagat
      781 ttcctgagcc ttgttttcac ggatcggcat ggcgaagcca ccgcagacca cgtcgcccag
      841 cacgtcgtag aacacgaagt ccaggtcttc gtcgtaggcg ccttcttctt ccaggaagtt
      901 gatggcggtg atcacgccac ggccggcgca gccgacgccg ggttccggac cgccggactc
      961 gacgcacttg acgccgccga agccgacgga gaggacgtct tcgagctcga ggtcttccac
     1021 ggagccggct tcagcagcca ggtgcatcac ggtggtctgg gccttgctgt gcaggatcag
     1081 acgggtggag tcagccttcg ggtcgcaacc cacgatcatc accttcttgc cggcctcggc
     1141 gagggcagca acgaggttct gggtggtggt ggacttgccg ataccgccct tgccgtagat
     1201 ggcgcattga cgcagtttag ccatggtgta atctccttaa tctgtcagtc gatttgtgag
     1261 tcgattggtg cgatgaaaca cagtgagagt cgcaactcac ctatcgcatg gactgtgcca
     1321 ggtcgactga cagcctctaa cttgctgatc tgcaaggaaa taaattcaaa gtacgtgtga
     1381 gagtgggctg tgtcgtcatc cgacaaagcc tcacaaagcc cacaatgccc ctacgctttg
     1441 taggggtggc gacaaaggga tgtgtggggg gaggacgacg acaccgggcc tccttgaatg
     1501 gcccggacgc attgacaccg cgcctgcggt gcgggcaggc ggtccgtcag gttcggacga
     1561 cgccggggac aggcccggcg cgcatgtgcg ggcgggttgg gcgtacatct gtatacagcc
     1621 gacgtaatgg tgtgcctggg cattgcgtgc ctgcagcgcc cgcgcggcca ccgcatgggg
     1681 cttacaggtg attacgccgg aactcgcgcg aacgcgcggg tgccccacat tctgtcggac
     1741 gcctcgtgcg gctggttcgg gggcgtggtg ccacgcccac gcgcgtggtg gagcggggga
     1801 tgaaatccac gaccggtcgc tcggggcccg gctgccggat ccctcgttcc acgccgacgc
     1861 cagggggcgt agcggtcgtg tcacaaaccc cacaatggca cgaacctgac gttggggagt
     1921 ggatctaaca aactgatcat aaagggattg ttcgcgggcg tggatattgc tggtgagggc
     1981 cgatacccca tctccggaga attccatgac ccccgaactg cgcgccacgc tgctggccgg
     2041 catccaggac ggttccatcg ttccttacct gggcgcaggt gtgctggctg acgtcaagaa
     2101 cgtcgccacc ggcgaaccca tccccgctac cagcgacgcc ctcatcattg ccatgaacgg
     2161 cggcaagccc atggcgccca agctgatgta cgagttcccc cgcgccgcca tgaacgtgga
     2221 gctcaagcgc ggccgcagca cggtcaacaa gtttctgacc cgcacctacg gcgagacgca
     2281 atggacccgc ggcgcggtgc acgactggat caaggcgatc cgtccccgct acgtcatcga
     2341 catcaaccgg gacacccagc tgcaggactc ctacgccgat gtgccccaca acctcatcgt
     2401 cggcatcgcc cgcatcggcg gcaccaccct gcgctacaag atcttcttct gggacggcag
     2461 cagctaccag cgcgccgaag agctcaagct cgaacatccc atcctcttca agcccgtcgg
     2521 cacgccgcgg cccgagccca cctacatcgc ctccgacgcc gactacgtgg atttcatcac
     2581 cgaactgatg ggcggcttct ccattccgcc ccagctcaag gaattccgca agggcaagca
     2641 atacctgctg gccggcctgc gcctcaatcg cgacaccgag cgcatggtga tggcggacat
     2701 catctacagc gccgccgaac cggctggctg ggtcttcatc gccgagccca ccgacaagga
     2761 acgccgctac tgcaagaaga tcggtctcga aatcgtcgac gccgacatct tcgacctcat
     2821 cggcgagccc cagcccgcct gaccgctttc aatccaggga gtcccatctc atgctgctcg
     2881 accgctacga acaatacttc ttcaacgcca acttccgcga agccgccgac gaggccggct
     2941 gggaagccta tcgcggcgaa ctggtcctga aggaaggcga gatcgccgac gacaagggcc
     3001 gccgcaagcc gccccacgaa gtcctcaagc aggtcgtcgt gctcgccaag ggcgatgagc
     3061 tcaagctcat ctccggccat ctggatgcgc tccagcacat ccccggcttc gtcgagaagt
     3121 acgctgccga cctcaaggcc gacaccatcg cggtgctctt cgctgtcaac atcgacgacc
     3181 ccttcctcgc caagatcggc gaaggcacgg tggccttcat ccccctggtg cagggcatgg
     3241 cctggaacga actgatcgac ctggtggccc tcgaaaaggg tgacttcaag ggtcagagcg
     3301 ccgccgagaa ggtggtcacc gtcttcaatg gcttcaaggg ccacaagttc aagtaccccg
     3361 agacgaccct cgacgacgcg ctgaagtcca ccaacaacgc caagcgcgag atgcacggcg
     3421 ccgtctgatg ccctgggacg acgcccaatc cctgggggtg gcggacatgg acgccaccca
     3481 ccgggaattc gtcgaacatg tggacgccct cgcgtccgcc gacgattctg cctttcccgc
     3541 gctcttcctg agcctgcagg aacacacccg cctgcacttc gagcacgaag gcaagctcat
     3601 gaaagcctgc cgcttccctg ccatcggtga gcacaacagc gaacacctgc gcgtactggg
     3661 cgaattcgcc cacttcgccc gcggtgtcaa tgcgggccgc ctgaccccgg cccgcgccta
     3721 catccaggcc ctgccaggct ggttcgccac ccacctggca acgatggacg cagcgctggc
     3781 ggggtgtctg aagcgcagtc ggggggagta gggcgcgggc cgggtgtgct tggccgatgg
     3841 gcttcggtgg cgcagaactg gggc
//
LOCUS       DAOQMM010000165         3819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_15784_length_3819_cov_44.988310, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000165 DAOQMM010000000
VERSION     DAOQMM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15784_length_3819_cov_44.988310"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLH85_16565"
     CDS             <1..452
                     /locus_tag="PLH85_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB66232.1"
                     /translation="PRPAPTTPTAPTAPGTTAPAEPLTRQDAPTGSAASAPSSAPTPA
                     RDAVAPSLVAPRFDADYLANPAPPYPGMSRRLGETGRVLLRVRVGSDGQPLDVGIAQS
                     SGFARLDEAARAAVARWRFVPARQGDQPVVASVQVPIVFTLNNKELP"
     gene            449..1183
                     /locus_tag="PLH85_16570"
     CDS             449..1183
                     /locus_tag="PLH85_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB66233.1"
                     /translation="MNESASAGLQLGQIWAQGDAIAHAVALLLLLMSLASWSVILIKG
                     WTLLRLRRLSTRSAQFWHCTRLEDGLTVLGPDGPDNPFRHLTERSTEAVAHHARHRDE
                     LHGVLDLNDWLSSSMRQTMDESTRRMQTGLSILASVGSTAPFVGLFGTVWGITHALTG
                     IGLSGQASLDQVAGPVGESLVMTAGGLAVAIPAVLAYNALTRGNREIVARLRHFAHDL
                     HAYLITGGKLRKRPVPPLDAPRQEAA"
     gene            1180..1602
                     /locus_tag="PLH85_16575"
     CDS             1180..1602
                     /locus_tag="PLH85_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB66234.1"
                     /translation="MSPHGRFDDDADEGLLAEINMTPLVDVMLVLLIVFIITVPVIHH
                     AARLDLPTASSQPEDTRPPRITLSMQADGHVAWNGESLTRDGLESRLTDAARAQPQPE
                     LHLQVDRGTPYDHVAHLLADASRLGLARVAFVTEPGRR"
     gene            complement(2081..2365)
                     /locus_tag="PLH85_16580"
     CDS             complement(2081..2365)
                     /locus_tag="PLH85_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66235.1"
                     /translation="MGSSCNESYAEFLDSLKAAGVVIRNEDEVRERLAESQRWRNAFM
                     TLAANGRTIGIEFSVAGKSKNVGAVKRVLAQHAFPVEKEEALVAQLVADH"
     gene            complement(2480..3625)
                     /locus_tag="PLH85_16585"
     CDS             complement(2480..3625)
                     /locus_tag="PLH85_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HNB66236.1"
                     /translation="MRISSNFDSGAIEVVSVDRADDIRLRLRADQGIEGDGAFRQWFH
                     FRVHGGAGAALRMVFENASEAAYPDGWPGYRCVASYDRRNWFRVSGTHYVDGQLVVEH
                     TPERDSVYYAYFEPYSHERHLDLLGWAEMSPYASVSNLGATVDGRDLDVVTVGRPGPG
                     KQPVWITARQHPGESMAEWFVEGVLERLLDGADPVARKIREHAVLHIVPNMNPDGAFR
                     GNLRTNAAGVNLNRAWREPDPLRSPEVHLVRQAMEASGVSLFLDIHGDEALPYVFFST
                     AEEVPGFSDAQRARQAEFVAAFEAASPDFQREHGYLPGRFAEELLTLASKWVAYRFGC
                     VSLTLEMPFKDNALLPDGLTGWNGARSKRLGAAILGPILRHVSATGA"
BASE COUNT          627 a         1492 c         1145 g          555 t
ORIGIN      
        1 gcccccgccc ggccccaacg acgccaacag ccccgacggc ccccggcacg accgcgcctg
       61 ccgagcccct cacccgccag gacgccccga ccggcagcgc cgcatccgcg ccgtccagcg
      121 ccccgacgcc cgcccgcgac gccgttgctc cgtccctcgt cgccccccgc ttcgacgccg
      181 actacctggc caatccggcc ccgccctacc ccggcatgtc ccgccgtctg ggcgaaacgg
      241 ggcgggtgct gctgcgcgtg cgggtcggca gcgacggcca gcccctcgac gtgggtatcg
      301 cccagagcag cggcttcgcc cgcctcgacg aggccgcccg cgcggcggtc gcccgctggc
      361 gcttcgtccc ggcccgccag ggcgaccaac cagtcgtcgc cagcgtgcag gttcccatcg
      421 tcttcaccct caacaacaag gaactcccat gaacgaatcc gcctcggccg gcctccagct
      481 cggccagatc tgggcccagg gcgacgccat cgcccacgcg gtcgccctgc tcctgctcct
      541 catgtccctg gcgtcctggt ccgtgatcct gatcaaaggc tggaccctcc tgcgcctgcg
      601 ccgcctgagc acccgctccg cccagttctg gcactgcacc cgcctggaag acggcctcac
      661 cgtcctcggc ccggacggcc ccgacaaccc cttccggcat ctcaccgagc gcagcaccga
      721 ggccgtcgcc caccacgccc gccaccggga cgagctgcac ggcgtcctcg atctcaacga
      781 ctggctctcc agctccatgc gtcagaccat ggacgaatcg acccggcgca tgcagaccgg
      841 cctgtccatt ctcgcctcgg tcggcagcac cgcccccttc gtcgggctgt tcggcaccgt
      901 gtggggcatc acccacgctc tcaccggcat cggtctgtcc ggtcaggcca gcctcgacca
      961 ggtcgccgga ccggtgggcg aatccctggt gatgaccgcc ggcggtctcg ccgtcgccat
     1021 ccccgcggtg ctcgcctaca acgccctcac acggggcaac cgggagatcg tcgcccgcct
     1081 gcgccacttc gcccacgacc tccacgccta cctcatcacc ggcggcaagc tgcgcaaacg
     1141 cccggtcccg cccctcgacg ccccccgcca ggaggccgca tgagccccca cggacgcttc
     1201 gacgacgacg cggatgaagg cctcctcgcc gagatcaaca tgacgccgct ggtggacgtg
     1261 atgctggtcc tgctgatcgt cttcatcatc accgttccgg tcatccacca cgccgcgcgc
     1321 ctcgacctgc ccacggcgag cagccagccc gaagacaccc gtccgccccg catcaccctc
     1381 agcatgcagg ccgacggaca tgtggcgtgg aacggcgaat ccctgacgcg cgatggcctg
     1441 gaaagtcgtc tgacagacgc cgcccgtgcc cagccccaac cggagctcca tctccaggtc
     1501 gaccgcggta cgccctacga ccacgtcgcc cacctgcttg ccgacgccag ccggctgggc
     1561 ctggcgcggg tggccttcgt cacggagccg ggccgacgct gagcacagcg ccccgccagc
     1621 ccgagacctt gacctgttcc gcgacgaagc gctcgcttgc gacacgtcac gccaaagcgg
     1681 accccgtttg cccggatggc cgcggtgcgc acgagaagcc ggagcgacac ccggtcaggc
     1741 ccggtagcca gtggggacag acatggcggg gggagacgga tcgaccggac accaatcccg
     1801 cccagccccc gacgcggaaa cgtccggccg cgcagagcca ggcatccttc ccccgccacc
     1861 gcgtgttcac cgcagcgtgg cgggggcgca gcgtcgagtg caccggcaca ggggtgaatc
     1921 ggtggagcag cccgcggcgc caatcaacgg gacgcgcctg tgcccccgcc gtaggcgtgc
     1981 cgggcgccca aaagaaatgc cgtccggctg gggccggacg gcatcggcat cttctttcga
     2041 tccgtgcgcg ggcggcggag acacccgccg caccggggga tcagtggtcg gctacgagtt
     2101 gggcgaccag cgcttcttcc ttttcaaccg ggaaggcgtg ctgggccagc acccgcttca
     2161 ccgcgcccac gttcttggac ttgccggcga ccgagaactc gatgccgatc gtccggccgt
     2221 tggcagccag ggtcatgaac gcgttgcgcc agcgttggga ttcggccaga cgttcacgca
     2281 cttcatcttc gttgcggatc acaacacctg cggctttgag ggagtccaga aattcagcgt
     2341 aggactcgtt gcaagaactg cccatcgaca tctcctatgg tctggtgcgt tgcacccgta
     2401 ctgccactaa cgcacacgga aaccgtcagg atgacaggcc gccgcaaaat ttctctcaaa
     2461 atgtttccgg acggggaact tatgcgcctg tcgcactgac gtgccggagg atcggcccca
     2521 ggattgccgc gccaaggcgt ttgctgcgcg ccccgttcca gcccgtcagc ccgtcgggca
     2581 gcagggcgtt gtccttgaag ggcatttcca gcgtaagcga cacgcaaccg aagcgatacg
     2641 ccacccattt ggacgccagg gtcagcaatt cctctgcaaa acgccccggc agatagccgt
     2701 gttcacgctg aaaatccgga ctcgcagcct cgaaggcggc gacgaattcc gcctgacgcg
     2761 cccgctgcgc atccgaaaac cccggcacct cctcggccgt cgagaagaac acgtagggca
     2821 gcgcctcgtc gccatggatg tcgaggaaca gggacacccc gctcgcctcc atggcctgcc
     2881 ggaccagatg gacctccggg ctgcgcagcg ggtccggctc gcgccacgcc cggttcaggt
     2941 tcaccccggc cgcgttggtg cgcagattgc cgcggaacgc gccatccggg ttcatgttcg
     3001 gcacgatgtg caggaccgca tgctcacgga tcttgcgcgc caccggatcg gctccgtcca
     3061 gcagacgttc cagcaccccc tccacgaacc actccgccat gctctcaccc ggatgctggc
     3121 gcgccgtgat ccacaccggc tgcttgccgg gccccgggcg ccccaccgtc accacatcca
     3181 gatcgcgccc atccaccgtg gcgcccaggt tgctcaccga ggcatagggc gacatctccg
     3241 cccagcccag cagatccaga tggcgctcat gggaataggg ctcgaagtag gcgtaataga
     3301 cgctgtcccg ctcgggcgtg tgctccacca ccagctgccc gtccacataa tgggtgccgc
     3361 tcacccggaa ccagttccgg cggtcgtagg acgccacgca gcgatagccc ggccagccgt
     3421 ccggataggc cgcctccgac gcgttctcga agaccatccg cagcgccgcg cccgcgcccc
     3481 cgtgcacccg gaagtggaac cactgccgga aggcgccgtc gccctcgatc ccctggtccg
     3541 cccgcagacg cagacggatg tcgtccgccc gatccaccga caccacctcg atggcgcctg
     3601 aatcaaaatt gctgctgata cgaatcgtca tggctgctcc cggcgtccaa agccggcact
     3661 atagcggccc ggtcccgatc ccggcagcag caggcccgct cggccctgtg gccaacgtga
     3721 caaggacttt tcaaacctgc gaccctcgtc tataatgcgc agcttgtcgg ggcgtagcgc
     3781 agcctggtag cgcacttgca tggggtgcaa ggggtcgcg
//
LOCUS       DAOQMM010000166         3775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_16096_length_3775_cov_45.700806, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000166 DAOQMM010000000
VERSION     DAOQMM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16096_length_3775_cov_45.700806"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLH85_16590"
     CDS             complement(<1..394)
                     /locus_tag="PLH85_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNB66237.1"
                     /translation="MPLVHISLRRGKSSHYLRQIADGVHRALVDAFEVPPDDRFQTLR
                     EFDGEALIVDPHYLGGPRGADFVLVEITAGRPRSTATKAAFYRRLAEGLQASPGLHPA
                     DLMVVITTTQRDEWSFGHGRATLLQPEAS"
     gene            complement(405..725)
                     /locus_tag="PLH85_16595"
     CDS             complement(405..725)
                     /locus_tag="PLH85_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB66238.1"
                     /translation="MHAPHTYGRQAMGDEALKLAQLTDDVLFNDVWERPELSKRDRSL
                     VTVAALLALYRLEQLPGHLVRALENGVAREELVEVITHLAFYAGWPAAHSAIQRLNAI
                     PRQG"
     gene            817..1731
                     /locus_tag="PLH85_16600"
     CDS             817..1731
                     /locus_tag="PLH85_16600"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB66239.1"
                     /translation="MNDRVPRITFDLDALRSFVTGIELGGFAKAADRVGRSASAVSAQ
                     LKKLEDQIGAPILQKSGRGRILTPTGECFLGYARRLLALNDEAAIAVRGVELSGSVRI
                     GLQEDFGEHLLSRMLGDFARAHPNVTVEVHLARNAALVRRMEEGRLDLALSWDTGADW
                     PYSETLGQLPLCWIGPAATQATDPTPSGPLPLVLFEAPCVMRSAATAALDAAGRAWRV
                     AVSSASLPGIWTAVGAGLGVTVRTRVGLPGHLVVRNDLPALPRVGLVLHRATDPAPEA
                     VKRLADIVVDTVADMGIDRPRHSAGEEP"
     gene            complement(1962..2429)
                     /locus_tag="PLH85_16605"
     CDS             complement(1962..2429)
                     /locus_tag="PLH85_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNB66240.1"
                     /translation="MSLSSLLRSLVLCAGGLAALIPVGVRAQSSMPQVELTVGMFRIE
                     AEVAATLGDRMQGLMQRRQMAANHGMVFVFTEDQRHCMWMKNTLLPLSVAFMDEQGKI
                     LNIEDMEPQTEDNHCAARPARFALEMNKGWFRQKGIAPGTTIRGVAPLAGAGR"
     assembly_gap    2518..2617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3203..3610
                     /locus_tag="PLH85_16610"
     CDS             3203..3610
                     /locus_tag="PLH85_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66241.1"
                     /translation="MTAASNSRIQSSTLEDDIDAFLALETISTYAPNNPEHSLPSITA
                     VYQKLRADQASVIRAQIAHDTARDNLIATEREFHQLMLGVKSQVRGQYGPNSNEVAAL
                     GLKKTSERRTPTRSSKGTPGTSGTLGAPETPTT"
BASE COUNT          734 a         1289 c         1061 g          591 t
ORIGIN      
        1 acgaagcttc gggctggagc agcgtcgcgc ggccgtgacc gaaggaccat tcatcccgct
       61 gggtggtggt gatgaccacc atcagatccg ccggatgcag accggggctg gcctgcaggc
      121 cctcggccag acggcggtag aaggccgcct tggtcgccgt gctgcgcggc cggcccgcgg
      181 tgatctccac cagcacgaaa tccgcgccgc gggggccgcc gagataatgg ggatcgacga
      241 tcaaggcctc cccatcgaac tcccgcagcg tctggaagcg gtcgtcgggc ggcacctcaa
      301 aggcgtccac cagcgcccga tgcacgccgt cggcgatctg ccgcaggtaa tgggacgact
      361 tgccacgacg caggctgata tggacgaggg gcatggtggg cacctcatcc ctgacgcgga
      421 atggcgttca gccgctggat ggccgaatgg gccgccggcc agcccgcgta aaaggccaga
      481 tgggtgatga cttcgaccag ctcttcccga gcgacgccat tctccagcgc ccggaccaga
      541 tggccgggca actgctccag gcggtacagc gcgagcaagg ccgccacggt caccagcgaa
      601 cggtcgcgct tggacagttc cggacgctcc cacacgtcgt tgaacagcac atcgtcggtc
      661 aattgggcca gcttgagggc ttcatcgccc atcgcctgac ggccataagt gtgcggggca
      721 tgcatggtgt tctcctggaa acgtttgacg acgccagaat atttccgcac catcatcccg
      781 tctatctgaa acatttgaat cgatagttca acaaaaatga acgatagagt cccccgcatc
      841 accttcgacc tggacgcatt gcgcagtttc gtcacgggca tcgaactcgg cggctttgcc
      901 aaggctgccg accgcgtggg gcgctcggcg tccgccgtca gtgcccaatt gaagaagctg
      961 gaggaccaga tcggcgcgcc catcctgcaa aagtccgggc gcggacggat cctgacgccc
     1021 accggggaat gttttctcgg ctacgcgcgg cgcctgctcg ccctcaacga cgaagcggcc
     1081 atcgccgtgc gcggcgtcga actgtcgggc agcgtacgca tcggcctgca ggaagatttc
     1141 ggcgaacacc tcctgtcacg catgctcggc gattttgcgc gggcccatcc gaatgtgacc
     1201 gtcgaagtcc atctcgcgcg caacgcagcg ctggtacgcc ggatggagga agggcgtctc
     1261 gatctggccc tgagctggga caccggcgcg gattggccgt acagcgagac gctcggacaa
     1321 ctccccctgt gctggatcgg cccggccgca acgcaggcga cggacccgac cccctcgggc
     1381 cccctccccc tggtgctctt cgaagcaccg tgcgtgatgc ggagcgccgc cacggcggca
     1441 ctcgacgccg ccggccgggc gtggcgcgtc gccgtgtcca gcgccagtct gcccggtatc
     1501 tggacggccg tgggcgccgg attgggcgtc acggtacgca cccgcgtcgg cctgcccggc
     1561 catctcgtcg tacgcaacga cctgcccgcg ctgccacggg tcgggctcgt actccatcgc
     1621 gcaaccgacc cggcgccgga agcggtcaag cgattggccg atatcgtggt ggacacggtc
     1681 gccgacatgg gcatcgacag accccggcac tccgcaggcg aagaaccctg aatcgcgccg
     1741 cccgacgatg ccagcggggc cgccgggcgc acacgtgaac acccctgacc cgcgaacgcc
     1801 gcgacgcggc gggcgcaggc atcacccgcc caggcaccga tcaggcgcac gccacgcgga
     1861 cgtagccccc gcgcaccccg ccagcctccg tccccatcct gtccgaacac atcaaacggg
     1921 tgccggcgac cgccgcggac cgctgcaaac agcgtgcgcc ctcagcgccc cgcgcccgcc
     1981 agcggcgcca cgccgcggat cgtcgtgccc ggcgcaatcc ccttctggcg gaaccagccc
     2041 ttgttcatct ccagcgcaaa acgggccggg cgggccgcgc agtggttgtc ctcggtctgg
     2101 ggctccatgt cctcgatgtt gaggatcttc ccctgctcgt ccatgaaggc caccgacagg
     2161 ggcagcagcg tgttcttcat ccacatgcag tgccgctggt cttcggtgaa gacaaacacc
     2221 atgccgtgat tggccgccat ctgccggcgc tgcatcaggc cctgcatgcg gtcccccaag
     2281 gtggccgcca cctccgcctc gatgcgaaac atccccaccg tgagctccac ctggggcatg
     2341 gatgactggg cccgaacgcc cacgggaatc agagcggcaa gccccccggc acacagcacg
     2401 agggaacgca gcaaggacga cagcgacatg gaaggacatc cggcaggcaa caaaggagcc
     2461 gcgaggatag cccagccagc cccgtccggc atagggccgg gcgcggcccg gtcacccnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac atcatcccac cgcctccaat
     2641 ctcttccacg cagcgtcaaa gcgctttcac gatagtctgt agtcgatatt cggtgaataa
     2701 agatcgaata cagactatcg aatcgcttct ttgatagtcc ctaccctttc tttgttttcc
     2761 aaatatcgaa tagcgactat caaatgtctt ctttaatagt ctctaccctt tctttgtccg
     2821 ccagagatcg agtagaaact atcaaagaag cactttacga ccgcccagcg cttcttgagc
     2881 cctgccgccg ccttcccggc cgccccagaa aggactccca cgccacgtgg aagaggatga
     2941 acgttcacga caaggactcg gccgccacac caaagaggaa tcgacggcct gcgcacgcct
     3001 gatggtttgc tgtgtcagcc atttccccca aaagcgccga tggacattcc aatacattac
     3061 gcatagcgat ggatttcccc acaaggcaat cgccgccgac gcctattcat cataaaatcc
     3121 caaagtacta tgcaataaaa ttatgctttg ataatctaca caggcggaca tccaccgcat
     3181 tcattcaact ggaggagaca ccatgaccgc tgcgtccaat tcgcgcatcc agtccagcac
     3241 cctcgaagac gacatcgacg ctttcctggc tttggaaacc atctcgacct acgcccccaa
     3301 caacccggag cacagcctcc ccagcatcac cgccgtctac cagaaactgc gcgccgatca
     3361 ggcctccgtg atccgcgccc agatcgccca cgacaccgcc cgcgacaacc ttatcgccac
     3421 cgagcgggaa ttccaccagc tcatgctcgg cgtcaagagc caggtcagag gccagtacgg
     3481 ccccaactcc aacgaagttg ccgccctggg gctgaagaaa acctccgaac gccgcacccc
     3541 cacgcggagc agcaagggca cgccgggaac atccggcacg ctcggagcac ccgaaacgcc
     3601 gacgacctga atcgcgtcga cgcattgcag caaaacggcc cggtaacggg ccgttttttc
     3661 tgtgcgtggc agaccggctc aggctacggc ctacagatat cgatgaccga ttccctccgc
     3721 actgccattg cggcaggctg taggtttcat ttgtataact tttgccatac atacc
//
LOCUS       DAOQMM010000167         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_17641_length_3570_cov_43.828165, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000167 DAOQMM010000000
VERSION     DAOQMM010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17641_length_3570_cov_43.828165"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..308)
                     /gene="minE"
                     /locus_tag="PLH85_16615"
     CDS             complement(51..308)
                     /gene="minE"
                     /locus_tag="PLH85_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNB66242.1"
                     /translation="MSLLSYFFGNKQKTAAVAKERLSLIIAHERGTNRAPDFLPALQR
                     ELIEVISKYVNVGPNDIKVQLEKQDNCEILEVNITLPEQPK"
     gene            complement(310..1125)
                     /gene="minD"
                     /locus_tag="PLH85_16620"
     CDS             complement(310..1125)
                     /gene="minD"
                     /locus_tag="PLH85_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNB66243.1"
                     /translation="MARVIVVTSGKGGVGKTTTSASFASGLALRGFKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVLNGEANLHQALIKDKHCENLFVLPASQTRDKDALTEEGV
                     EKVLKDLEHMGFDYVVCDSPAGIERGAVMALTFADEAIVVTNPEVSSVRDSDRILGIL
                     QSKSRRAIEGREPVKEHLLVTRYSAKRVEEGEMLSYKDVQELLRIPLIGAIPESDDVL
                     QASNQGTPAIHLKDSDVATAYQDVVARFLDEDRPLRFVTYEKPGLLKRLFGGK"
     gene            complement(1159..1953)
                     /gene="minC"
                     /locus_tag="PLH85_16625"
     CDS             complement(1159..1953)
                     /gene="minC"
                     /locus_tag="PLH85_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNB66244.1"
                     /translation="MSDPLLQSKLIEFKGATLPVMTAHLRDTDAVRLADALHMMLGGM
                     PDFFAGEPAILDLAGLTTVPERIDWTALLSMLRRYQVQPVAVRHLPAGLEDGARRAGL
                     ALLGPESAVTERPVARPAPAPAAAPKPAPEPAPAAVTPPPAPAAVPTATLVVDKPLRS
                     GQQVYAKGGDLVLLAGVSPGAEVIADGSIHCYGPLRGRALAGARGDHTARIFSTNFGP
                     ELVSIAGVYRTFERGIPQAVAGRGTQVRLTGSDALHTLEITPLATD"
     gene            complement(2096..2980)
                     /locus_tag="PLH85_16630"
     CDS             complement(2096..2980)
                     /locus_tag="PLH85_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNB66245.1"
                     /translation="MSRPDSFIQRFLLEELDIRGAVVRLDQVWQALLENRDYAPPLRD
                     LLGAMSAVTTVICGNLKQPGRITVQLQGEGPVRLLVVDCADTLNLRGYAKTRDDLSAP
                     LPPGGLGELVGDGQLLLSLDMPGLARPYQSYVPLEGDSVAEVFEHYLTQSEQQPALLR
                     TASSGTVAACLFLQKLPDADLKDPDGWQRVHHLVATLRNEELLALPPEALLTRLFHEE
                     QVRLFDPRDVTHDWPLDWEKVRRMLRSLGRDELDKIIAEHGEIHIHDDLSNHEYRLSA
                     ADVAELFAEEPPEQRTLH"
     gene            complement(2977..3342)
                     /locus_tag="PLH85_16635"
     CDS             complement(2977..3342)
                     /locus_tag="PLH85_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HNB66246.1"
                     /translation="MSTTVYGIKNCDTMKKAFAWLDEHGVAYTFHDYKKSGIDSARLH
                     AWSKAVGWKTLLNTRGTTWRKLPPERQALDTQSAAVALMGEFPSVIRRPVVETAGGQL
                     LVGFDPATFASFLGGEPRA"
BASE COUNT          526 a         1041 c         1381 g          622 t
ORIGIN      
        1 ttttaagtcc gctgtgttta ccgatttcac catccgggcg gcgggcggcc ttatttgggc
       61 tgctccggca gggtgatgtt cacttcgagg atctcgcagt tgtcctgctt ttcaagctgg
      121 accttgatgt cgttggggcc gacattgacg tacttggaga tgacctcgat gagctcccgc
      181 tgcagggccg gcaggaagtc cggggcgcgg ttggtgccgc gctcgtgggc gatgatcagg
      241 gacagccgtt ccttggcgac ggcggcggtc ttctgcttgt tgccgaagaa gtaggagagg
      301 agggacatct cacttgcctc cgaagagccg cttgagcagg cccggctttt cgtaggtgac
      361 gaaacgcagg gggcggtcct catcgaggaa gcgggccacc acgtcctggt aggccgtggc
      421 cacgtcactg tccttgaggt ggatggcggg cgtgccctgg ttggaggcct gcagcacgtc
      481 gtcggattcg gggatggcgc cgatcagggg aatgcgcagg agttcctgca cgtccttgta
      541 cgagagcatc tcgccttctt cgacgcgctt ggcggagtag cgggtgacga gcaggtgctc
      601 cttgaccggc tcgcggcctt cgatggcgcg gcggctcttg ctctgcagga tgcccaggat
      661 gcggtcggag tcgcgcacgg aggagacttc cgggttggtg acgacgatgg cctcgtcggc
      721 gaaggtgagg gccatcacgg cgccacgctc gatgccggcg ggggaatcgc acactacgta
      781 gtcgaagccc atgtgctcca gatccttgag gaccttctcg acaccttcct cggtgagcgc
      841 gtccttgtcg cgggtctggg aggcaggcag gacgaagagg ttctcgcagt gcttgtcctt
      901 gatgagggcc tggtgcaggt tggcctcgcc gttgaggacg ttgatgaggt catacaccac
      961 gcggcgttcg cagcccatga tgaggtcgag gttgcgcagg ccgacatcga agtcgatgac
     1021 cgcggtcttg aagccgcgca gggcgagccc ggaagcgaag cttgcactgg tggtggtttt
     1081 gcccacaccg cccttgccgg acgttacgac gatgacgcgt gccacgatat ccccttgttc
     1141 ctgaatgtga aaggccgctc agtcggtggc gagcggcgtg atttcgagcg tatggagggc
     1201 gtccgagccg gtaagccgga cctgggtgcc gcggccggcg acggcctgcg ggatgccccg
     1261 ttcgaaggtc cggtagacgc cggcgatgga gaccagctcg ggaccgaagt tggtgctgaa
     1321 gatgcgtgcc gtgtgatcgc cccgcgcgcc ggcgagggcg cggccgcgca ggggtccgta
     1381 gcagtggatg ctgccgtcgg cgatgacctc ggcgccggga ctcacgcccg ccaggagcac
     1441 cagatcgccg cccttggcat acacctgctg acctgagcgc aggggcttgt ccaccaccag
     1501 ggtggcggta ggtacggctg cgggcgcggg cggcggggtg acggccgctg gtgccggttc
     1561 cggcgcaggc ttgggcgcgg cggccggggc gggcgcggga cgggccaccg ggcgctcggt
     1621 gacggcggat tcgggcccga gcagggcgag cccggcgcga cgcgcgccgt cctcgagccc
     1681 ggcgggcagg tggcgcacgg ccacgggctg cacctggtag cggcgcagca tgctgaggag
     1741 ggcggtccag tcgatgcgct cgggcaccgt ggtgagcccg gccagatcga ggatggccgg
     1801 ttcaccggcg aagaaatccg gcatgccgcc caacatcatg tgcagggcat ccgccaggcg
     1861 cacggcgtcc gtgtcccgca gatgggcggt catgaccggc agggtggcgc ccttgaactc
     1921 gatcagtttg ctctgcagga gggggtcgga catggcggaa ggcggtcggg tgagagggtc
     1981 gggaacgggt caggcgccgg ggctgcgtaa gaggccgtcc cgcaggtgga aaacgggagc
     2041 ctgaaccggc taagtctact tttcagcagg gtttccggca agtaaacccc ggatttcaat
     2101 gaagggtgcg ctgttcgggc ggctcctcgg cgaagagttc ggccacgtcg gcggcggaaa
     2161 gtcggtattc gtggttggag aggtcgtcgt ggatatggat ttcgccgtgt tccgcgatga
     2221 tcttgtccag ttcgtcacgg cccaggctgc gcagcatgcg gcgcaccttc tcccagtcca
     2281 gcggccagtc gtgggtgacg tcccgcgggt cgaagaggcg cacctgctct tcatggaaga
     2341 ggcgggtgag cagggcttcg ggcggcaggg cgagcagttc ctcgttgcgc agggtggcca
     2401 ccagatggtg gacgcgctgc cagccatccg ggtctttgag gtcggcgtcg ggcagcttct
     2461 gcaggaacag gcaggcggcc acggtgccgg aactggcggt gcgcagcagc gcgggttgct
     2521 gttcggactg ggtgagatag tgctcgaaga cctcggccac gctgtcgcct tcgaggggca
     2581 cgtagctctg gtaggggcgc gccagaccgg gcatgtcgag ggacagcagc agttgtccgt
     2641 cgccgaccag ttcgccgagt ccgccaggag gcaggggcgc gctcaggtcg tcgcgggtct
     2701 tggcatagcc gcgcaggttg agggtgtcgg cgcaatccac caccagcagg cgcaccgggc
     2761 cttcgccctg gagctgcacg gtgatgcggc cgggctgctt gaggttgccg cagatgacgg
     2821 tggtgacggc gctcatggcg cccagcaggt cgcgcagggg cggggcgtag tcgcggttct
     2881 ccagcagggc ctgccacacc tggtcgaggc gtacgacggc gccgcggatg tccagttctt
     2941 caaggaggaa gcgctggatg aagctgtcgg gccggctcat gcgcgcggct ccccgccgag
     3001 gaaactggcg aaggtggccg ggtcgaagcc caccagcagc tggccgccgg cggtttccac
     3061 caccgggcgg cggatcacgc tggggaattc gcccatcaat gcgacggcgg cgctctgggt
     3121 gtcgagggcc tgacgctccg gcggcagctt gcgccaggtg gtgccgcggg tgttgagcag
     3181 ggtcttccag ccgacggcct tgctccaggc gtggagacgg gcgctgtcga tgccggattt
     3241 cttgtaatcg tgaaaggtgt aggcgacccc gtgctcgtcg agccaggcga aggctttctt
     3301 catggtgtcg cagtttttga tgccgtagac ggtggtgctc atggggctgg acaggacgcg
     3361 acgcccggtg gggcgtcgct ggaaaatcaa ggagatggac cggattctag ccggcccggc
     3421 ggcaggccgg gcctatttct tgagcgcctt ggcgaaggcc gtggcgaggg agccggccat
     3481 cgccggttcg cgggccggcc gggcggcctg ggggcccggg ccgttgcggg gcgcctcgcg
     3541 acgctcgccg gcggggcgtg cagcggcggc
//
LOCUS       DAOQMM010000168         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_18005_length_3528_cov_59.312986, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000168 DAOQMM010000000
VERSION     DAOQMM010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18005_length_3528_cov_59.312986"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="actP"
                     /locus_tag="PLH85_16640"
     CDS             <1..777
                     /gene="actP"
                     /locus_tag="PLH85_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764712.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB66247.1"
                     /translation="RKSVGWATVWIGYFYILTFIIGFGAICFVLTDPSFLDAKGALKG
                     GGNMAAIHLANAVGGNVFLGFISAVAFATILAVVAGLTLSGASAVSHDLYATVFKKGN
                     ADSAAELRVSRITTIVLGIVAVILGIAFEKQNIAFMVSLAFAIAASANFPVLFMSVLW
                     KDCTTKGAVIGGFLGLISAVGMTVLSDSVWVAVLGNPKGSAPFPYTSPALFSMSIGFV
                     GIWIFSLMDRSPAAQQEREDFAAQLIRSETGIGAASASSH"
     gene            1212..1886
                     /locus_tag="PLH85_16645"
     CDS             1212..1886
                     /locus_tag="PLH85_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB66248.1"
                     /translation="MRILLAEDDLIIADGLCRALKKAGYAVDHVPNGADADAALLGQT
                     FDLLILDLGLPKLSGIEVLRRLRARKCTLPVLILTAQDGIEDRVRGLDAGADDYLTKP
                     FALPELEARVRALTRRGTGQARCIEYARLAYDQADRVVKIDGQVVDLSAREIGLLEVL
                     LLRAGRLVSKEQLVDHLCGWGEEVSNNAIEVYVHRLRKKLEQSGVQIVTVRGLGYCLE
                     KPDVVA"
     gene            1873..3384
                     /locus_tag="PLH85_16650"
     CDS             1873..3384
                     /locus_tag="PLH85_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNB66249.1"
                     /translation="MWSPKWRRVADDGAADGKGFNSLFGEILDWMLAPLLFLWPISII
                     VTHNVANSIANQPYDQALAVNVRAIARLVRVEGSQARVNFPAPARLILRADEDDTVYY
                     QVLGFKGELVAGDKDIPTLQTPDSVVPDVVLFRDESIAGEDVRVAYQYLQPDAAAPRR
                     LVLLQVAETRNKRSTLASRVVTGVLLPQFAIIPIAVILVWLGLSRGIAPLNQLQRLIR
                     RRRPTDLSPISVSNVPEEVRPLIIAFNEMMARLEENLQAQQRFIADAAHQMRTPLTGL
                     RMQSDLALMETDPAQIHRSLQHISQSAERASHLINQLLSLARAEASYEKIYAVERVDL
                     EALLRAMLTDFVPRARQKAIDFGLETTGGPLVIDGNPVLLAELLKNLIDNALKYTPEG
                     GRVTVRTLATEKAIVEVEDTGIGVPEADRERVFERFYRVLGSSESGSGLGLPIVREIA
                     ELHRAHVSLNPNPAGRGTIAQVVFPRSRPTPEETAAQTTQALTAPGATQFPPA"
     gene            3453..3528
                     /locus_tag="PLH85_16655"
     tRNA            3453..3528
                     /locus_tag="PLH85_16655"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3486..3488,aa:Phe,seq:gaa)
BASE COUNT          590 a         1266 c         1078 g          594 t
ORIGIN      
        1 cgcaagtccg tgggttgggc caccgtctgg atcggctact tctacatcct gaccttcatc
       61 atcggtttcg gcgccatctg cttcgtgctg actgacccga gcttcctgga tgccaagggc
      121 gccctcaagg gcggcggcaa catggcggcc atccacctcg ccaacgcggt gggcggcaac
      181 gtgttcctgg gcttcatctc cgccgtggcc ttcgccacaa tcctggcggt ggtggccggc
      241 ctgaccctgt ccggcgcctc ggccgtgtcc cacgacctct acgccaccgt gttcaagaag
      301 ggcaatgccg attccgcggc tgaactgcgc gtgtcccgca tcaccaccat cgtgctgggc
      361 atcgttgcgg tgatcctggg gatcgccttc gagaagcaga acatcgcctt catggtgtcc
      421 ctggccttcg ccatcgcggc gtccgccaac ttcccggtgc tcttcatgag cgtgctgtgg
      481 aaggactgca ccaccaaggg cgcggtgatc ggtggcttcc tgggtctgat cagcgcggtc
      541 ggaatgaccg tcctgtccga ctcggtgtgg gtggcggtgc tgggcaaccc gaagggctcc
      601 gcgcctttcc cctacacctc cccggccctg ttctccatga gcatcggctt cgtgggcatc
      661 tggatcttct cgctgatgga ccgttcgccg gccgcccagc aggagcgcga agacttcgcc
      721 gcccagctga tccgttccga aaccggtatc ggtgccgcct cggcttccag ccactgagcg
      781 acatcgccgg agcggtcacc cgaccgctcc ggaccggacg caagacccgg cgtgccgaaa
      841 ggccgccggg ttttttccat tttcgacggg ggtgcgccgc gacggcacca aatcgatggt
      901 ccgggccctg tgctagactc tgcgggtttc ggaatggacg cgtgccgaca acccacgcag
      961 ctgcgcgggg cggaatcgat gcggaacggg tgcgccgggc tgtgtgcgtg cagtacgccc
     1021 gatggggcgt gcaggccggc aaaaaagacg gcgggggaag gtcccgtcgg gcgggctcaa
     1081 tcccccgagg cctcgtctgc agatgaaccg cagactcagc ccccaagacc gagtaaaagg
     1141 agaacagcga ggatgactat gacagtcccc gcttacttca aacttacacc tccatccttg
     1201 taaggtgagc gatgcgcatc ctgctggcgg aagatgatct catcatcgcg gacgggctct
     1261 gtcgcgccct caagaaggcc ggttacgcgg tggatcacgt gcccaacggg gccgacgctg
     1321 acgctgccct tctcgggcag accttcgacc tgctgatcct cgacctgggg ctgcccaagc
     1381 tgtccggcat cgaagtgctg cgccgcctgc gggcccgcaa gtgcacgctg ccggtattga
     1441 tcctgacggc ccaggacggc atcgaagacc gggtgcgcgg cctggatgcc ggtgccgacg
     1501 actacctcac caaacccttc gcgctgcccg aactggaggc gcgcgtgcgg gccctcacgc
     1561 gccgcggtac cggccaggcg cgctgtatcg agtacgcccg gctggcctac gatcaggccg
     1621 accgcgtcgt caagatcgac ggtcaggtgg tggatctctc cgcccgcgag atcggcctgc
     1681 tcgaagtcct gctcctgcgc gccggccgcc tggtgagcaa ggagcagctc gtggaccacc
     1741 tgtgcggctg gggcgaagag gtctccaaca acgccatcga ggtgtacgtc caccgcctgc
     1801 gcaagaagct ggagcaaagc ggcgtgcaga tcgtcaccgt gcgcgggttg ggctactgcc
     1861 tggaaaagcc ggatgtggtc gcctaaatgg cgcagggtgg cggatgacgg cgccgccgac
     1921 ggcaagggct tcaactccct gttcggggaa atcctcgact ggatgctggc gccgctgctg
     1981 ttcctgtggc ccatctccat catcgtcacc cacaacgtcg ccaacagcat cgccaaccag
     2041 ccctacgatc aggccctggc cgtcaacgtg cgcgccatcg cccgcctggt gcgggtcgag
     2101 ggcagtcagg cacgcgtgaa tttccctgcg ccggcccgcc tcatcctgcg cgccgacgag
     2161 gacgacaccg tgtactacca agtgctcggt ttcaagggcg aactggtcgc cggcgacaag
     2221 gacatcccga cgctgcagac gcccgacagc gtggtgcccg atgtggtcct gttccgtgac
     2281 gagtccatcg ccggagagga cgtacgggtc gcctatcagt acctccagcc cgacgccgcc
     2341 gcgccgcgcc ggctggtcct gctgcaggtc gccgagaccc gcaacaagcg cagcaccctc
     2401 gcgtcccgcg tcgtcaccgg cgtgctgctg ccccagttcg cgatcatccc catcgccgtc
     2461 atcctggttt ggttgggcct gtcccgcggt atcgcgcccc tcaaccagct ccagcgcctg
     2521 atccggcggc ggcggccgac ggatctgtcg cccatctccg tctccaacgt gcccgaggag
     2581 gtgcgtcccc tcatcatcgc cttcaacgag atgatggccc gcctggaaga aaacctgcag
     2641 gcccagcagc gcttcatcgc cgacgccgcg caccagatgc gcacgcccct caccggcctg
     2701 cgcatgcaga gcgacctcgc cctgatggag actgatcctg cacagatcca tcgctccctg
     2761 cagcacatct cccagagcgc cgagcgcgcc agccacctca tcaatcagct gctgtccctg
     2821 gcccgcgccg aagccagtta cgaaaagatc tacgctgtcg aacgggtgga cctggaggcc
     2881 ctgctgcgcg ccatgctcac cgacttcgtc ccgcgggcac gccagaaggc catcgacttc
     2941 ggcctcgaaa ccaccggcgg ccccctcgtc atcgacggca atccggtgct gctggccgaa
     3001 ctcctcaaga acctcatcga caacgccctc aaatacacgc cggaaggtgg ccgcgtcacc
     3061 gtgcgcaccc tcgccaccga aaaggccatc gtcgaagtcg aagacaccgg catcggcgtg
     3121 cccgaagccg accgggagcg cgtcttcgaa cgcttctatc gcgtgctggg cagctccgaa
     3181 agcggctccg gcctgggcct gcccatcgtc cgcgagatcg ccgagctgca ccgtgcccac
     3241 gtcagcctca accccaaccc ggccggcagg ggcaccatcg cccaggtcgt ctttccgcgc
     3301 agccgcccca ccccggaaga gaccgcagcc cagaccaccc aggccctcac cgcccccggc
     3361 gccacccagt ttccgcccgc ctgaaacctg gcgtccttcc gggattttct tgacaggacg
     3421 atgaaccaaa cctatactgc gcgcttcatc tcggccaggt agctcagttg gtagagcagc
     3481 ggattgaaaa tccgcgtgtc ggcggttcga ttccgtccct ggccacca
//
LOCUS       DAOQMM010000169         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_18305_length_3493_cov_45.846713, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000169 DAOQMM010000000
VERSION     DAOQMM010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18305_length_3493_cov_45.846713"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLH85_16660"
     CDS             <1..792
                     /locus_tag="PLH85_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB66250.1"
                     /translation="PDLSHANKLNMDVFVPALVFAALANKSFHVADFVPLLGATLFLV
                     GGCGLAGWALARGLGVAPRTLVPPLMFNNCGNLGLPLAVLAFGDQALAPAVAMFMVSN
                     LLHFSFGAWLLDHSISVAGVWKVPSVLATLAGIAVGLLGIEVWAPAMTAIRMLGDISI
                     PLMLFALGVRLTESRIGELRLGLIGAAARPLIGMALALLAMQLIPLSKSEQALLLVFG
                     ALPPAVLNYIFAERYHQEPEKVASIVLLGNVAALLFLPVALAIGL"
     gene            complement(799..1197)
                     /locus_tag="PLH85_16665"
     CDS             complement(799..1197)
                     /locus_tag="PLH85_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNB66251.1"
                     /translation="MKADEHPAERVRLDKWLWAARFFKHRSLATEAVDGGKIQLNGLR
                     VKPAKDVKPGDRVDIQIDATRFSVIVTGVAEKRGSAAVAQTLYTETAESIAARERQRE
                     ERRFAATPGDDLHGRPTKRDRRRIGRLQEG"
     gene            complement(1194..1736)
                     /locus_tag="PLH85_16670"
     CDS             complement(1194..1736)
                     /locus_tag="PLH85_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB66252.1"
                     /translation="MPMNDAPCLLVIDDDPAFNRVLSRALVQRGFEAYGAQDPESALA
                     LAREHQPEYVVLDLNLDGSSGLRLIEPLRAVNPDCRILVLTGYASIATTVDAIKLGAT
                     QYLAKPADVDAILKALNATDVLIDDDLAAGPMSVDRLEWEHIQRVLGEHEGNISATAR
                     ALKMHRRTLQRKLAKRPVKE"
     gene            complement(1727..2977)
                     /locus_tag="PLH85_16675"
     CDS             complement(1727..2977)
                     /locus_tag="PLH85_16675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB66253.1"
                     /translation="MPFLTDSPPGSADALRRLVGLRWLSVGAMAALLGAVPPLLDIAL
                     PVSALWPVVLGLAAVNGASVLRLRQGGEVTPVELFAQLCMDLAAWSALLYFTGGATNP
                     LISLLLPLVAIGAAILPAPYAWGLAVLAVAAYSLLWEFNEQLDIADSGLAVHWHLAGM
                     WLTFAVSAGVIVGYVVRMTALIRHRDQALAEAREERLRNERIVALGNLAAGAAHELGT
                     PLGTMAILAGELARNPRLDADTRADAALLGEQVAHCKAILGGLTLRAGSLRAEGGETQ
                     AADRWLDGIVERWRRLRPHVPLATHIDCGHPAPRLVADATLEQALHNLINNAADASPD
                     GVECEAALAGEALVVRILDRGPGLPEGCQPGQPTPATGRGLGIGLLLAFGAVERCGGR
                     IEFNPRPGGGTLATLTLPLSALCP"
     gene            3213..3431
                     /locus_tag="PLH85_16680"
     CDS             3213..3431
                     /locus_tag="PLH85_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HNB66254.1"
                     /translation="MAIKDIKAFSDKARIEPELKDKLAACQKIRELLTLAKDSGFNLD
                     EQELYPPNEPQFTADQLSERLVKALLRA"
BASE COUNT          477 a         1205 c         1264 g          547 t
ORIGIN      
        1 cccgacctct cccacgccaa caagctgaac atggacgtgt tcgtgccggc gctggtcttt
       61 gccgccctgg ccaacaagtc cttccatgtg gcggatttcg tgcccctgct gggcgccacc
      121 ctgttcctgg tgggcggttg cgggctggcc ggctgggccc tggcgcgggg gttgggggtg
      181 gcgccgcgca ccctggtgcc gcccctcatg ttcaacaact gcggcaacct gggcctgccc
      241 ctggcggtgc tggccttcgg cgatcaggcc ctggcgccgg cggtggccat gttcatggtc
      301 tccaacctgc tgcacttctc cttcggcgcc tggctgctgg accactccat cagcgtcgcc
      361 ggggtgtgga aggtgccctc cgtgctggcc accctggcgg gcatcgcggt cgggctgctg
      421 ggcatcgagg tgtgggcgcc ggccatgacg gcgatccgca tgctggggga catctccatc
      481 ccgctgatgc tcttcgccct gggtgtgcgc ctcaccgagt cgcgcatcgg cgagctgcgc
      541 ctcgggctga tcggcgccgc ggcgcggccc ctcattggca tggccctcgc gctgctggcg
      601 atgcagctga ttcccctgtc aaagtccgaa caggcgctcc tgctggtgtt cggcgccctg
      661 ccaccggcgg tgctcaacta catcttcgcc gagcgctacc accaggagcc ggagaaggtg
      721 gcctccatcg tgctgctggg caacgtggcg gccctgctgt tcctgccggt ggccctggcc
      781 atcggtctgt agccggcctc agccttcctg caggcggccg atgcggcggc ggtcgcgctt
      841 ggtggggcgg ccgtgcaggt cgtcccccgg cgtggcggca aagcgccgct cctcgcgctg
      901 gcgttcccgc gcggcgatgc tctcggcggt ctcggtgtag agggtctggg ccaccgcggc
      961 gctgccacgc ttctccgcca cgcccgtcac gatcacgctg aagcgggtcg cgtcgatctg
     1021 gatgtcgacg cggtcgccgg gcttcacgtc cttggcgggc tttacgcgca ggccgttgag
     1081 ctggatcttg cccccgtcca cggcctcggt ggccaggctg cggtgcttga agaagcgggc
     1141 cgcccacagc catttgtcga ggcggacgcg ctcagcgggg tgttcgtcgg ctttcattcc
     1201 ttgaccggcc gcttggccag tttccgttgc agggtacgtc ggtgcatttt aagcgcccgc
     1261 gcggtggccg agatgttgcc ctcgtgttcg ccgagcacgc gctggatgtg ctcccactcc
     1321 agccgatcca ccgacatggg cccggcggcc aggtcgtcgt cgatgagcac gtcggtggcg
     1381 ttgagggcct tgaggatggc gtccacgtcg gccggcttgg ccagatactg ggtggcgccc
     1441 agcttgatcg catccaccgt ggtggcgatg ctggcgtagc cggtgaggac caggatgcga
     1501 cagtccggat tgaccgcccg cagcggctcg atcaggcgca gcccggagct gccgtccagg
     1561 ttcaggtcca gcaccacgta ttccggctga tgttcccggg ccagggccag ggcgctctcc
     1621 gggtcctggg cgccgtaggc ctcgaagccg cgctggacga gggcccggct gaggacccgg
     1681 ttgaaggcgg ggtcgtcgtc gatgacgagg aggcagggcg cgtcgttcat gggcagaggg
     1741 ccgacagggg cagggtcagg gtggcgaggg tgccgccgcc ggggcgcggg ttgaactcga
     1801 tgcgcccgcc gcagcgttcc acggcgccga aggcgaggag caggccgatg cccaggccgc
     1861 ggccggtggc cggcgtcggt tggcccggct ggcaaccttc cggcaggccc gggccgcggt
     1921 cgaggattcg caccacgagg gcctcgccgg cgagggccgc ttcgcattcg acgccgtccg
     1981 ggctggcgtc ggcggcgttg ttgatgaggt tatggagggc ctgctccagg gtcgcgtcgg
     2041 ccaccaggcg gggcgccggg tggccgcagt cgatgtgcgt ggccaggggc acgtgggggc
     2101 gcagacggcg ccagcgttcc acgatgccgt cgagccagcg gtcggcggcc tgggtctcgc
     2161 cgccctcggc gcgcaggctg ccggcgcgca gggtcagccc gcccaggatc gccttgcagt
     2221 gggccacctg ttcccccagc agcgctgcgt cggcccgcgt gtcggcgtcc aggcgcggat
     2281 tgcgggccag ctcgccggcc aggatggcca tggtgcccag gggcgtgccc agctcgtggg
     2341 ccgcgcccgc cgccagattg cccagggcca cgatgcgctc gttgcgcagg cgctcctcgc
     2401 gggcctcggc cagggcctgg tcgcggtggc ggatcagcgc cgtcatgcgc accacgtagc
     2461 ccacgatgac ccccgccgac accgcgaagg tcagccacat gcccgccaga tgccagtgca
     2521 ccgccagacc cgaatcggcg atgtccagct gctcgttgaa ctcccacagc agggaatagg
     2581 ccgccaccgc cagcaccgcc aggccccagg cgtagggcgc cggcaggatc gccgcgccga
     2641 tggccaccag gggcagcagc agggagatca gcggattggt ggcgccgccg gtgaagtaga
     2701 gcagggcgct ccaggccgcc agatccatgc acaactgggc gaacagctcc accggcgtca
     2761 cctcgccgcc ctggcgcagg cgcagcacgc tggccccatt gaccgccgcc agccccagca
     2821 cgaccggcca caaggccgac accggcaggg cgatgtccag cagcggcggc accgccccga
     2881 gcagcgccgc catggccccc acggacagcc agcgcaggcc cacgaggcgg cgcagggcat
     2941 cggcggagcc gggcggagag tcggtcagga agggcatcgg gcgattatcc ccgaagcggg
     3001 gcggcggcat ggcaggtcga agcacggcca gccactttaa cgctcccgcc cggcccccgg
     3061 aatgtggcgg aaggtcgcag gcggcgcggt ctttgctaag gaaggggccg acagccggaa
     3121 atgtgcactt ctgcggcagc tttgcgacaa agccgccggg tcttgtcgcg tttccggcag
     3181 tcacgatccg ttcaaaccgt acaggaacca ccatggctat caaggacatc aaggccttct
     3241 ccgacaaggc ccgcatcgaa ccggaactca aggacaagct ggccgcctgc cagaagatcc
     3301 gcgaactgct caccctggcg aaggactccg gcttcaacct ggacgagcag gagctctatc
     3361 cgcccaatga accgcaattc acggcggatc agttgtcgga gcggctggtg aaggcgctgc
     3421 tgcgggcctg atcggctttt tgccgcaagc ggcggcacgg tgattgacag cgtgtcgcct
     3481 ctggaggcgg tag
//
LOCUS       DAOQMM010000170         3488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_18345_length_3488_cov_61.177687, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000170 DAOQMM010000000
VERSION     DAOQMM010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18345_length_3488_cov_61.177687"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLH85_16685"
     CDS             complement(<1..242)
                     /locus_tag="PLH85_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66255.1"
                     /translation="MSPLWPESIRLHIGPDCLRLTRAGGAASESPLAADWAVGLATLP
                     VLPRRARLHVTVADRHARYLRIDWPAGLRAAERAAFT"
     gene            complement(223..1890)
                     /locus_tag="PLH85_16690"
     CDS             complement(223..1890)
                     /locus_tag="PLH85_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNB66256.1"
                     /translation="MPFSAAELAAAARRAGQDGVRLVEVLARPDGAAASDDFPARLAA
                     TVGLTRLTAAELFALAPDFDGLSFDEALRREALLGRDAGGHTVLAVADPFDTDLVDGL
                     GARLLHPFVVRLADRDDLTACLARHEESRRVVAGMLAGREEAATGDAGEDLSLRRIAG
                     DASPVVKLVNSTLYDAIKQGASDVHLESVPGGLVIKYRVDGVLARVGGVQGTELAEQA
                     VSRLKVMAELDIAERRVPQDGRFKVQVSGRPIDFRVSIMPSIHGEDAVLRILDKEHLT
                     RELAGLTLETLGFDPAVRQQLRHMAGEPYGMLLVTGPTGSGKTTSLYATLSETNTGHD
                     KIVTIEDPVEYQLPGVLQIPVNEKKGLSFARGLRSILRHDPDKIMVGEIRDAETAEIA
                     VQAALTGHLVFTTVHANNVFDVIGRFMHMGIDPYNLVAALNGVVAQRLIRVNCPACST
                     ASEPDAALLRESGLATPADVQGFAFRAGSGCGQCRGTGYRGRKAIAEVLLLDDELRDL
                     IIARAPLRQLKDTARRRGFVSLREAGLQRVREGLTTLQELNRVTFVA"
     gene            complement(1902..3080)
                     /locus_tag="PLH85_16695"
     CDS             complement(1902..3080)
                     /locus_tag="PLH85_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNB66257.1"
                     /translation="MRFRIKAVGNGAAVQELEVEAVDEASARTLLAGRGLAVLSATAL
                     RGGGGRRTGFPLMLFSQELLALLKAGIPLADGIAALAEKESRPAVQAVLTALGSGLRE
                     GRTFSACLESQPQAFPDLFVATLRAAERTSDLEPALARYIDYQRQLELVRSKLVSAAV
                     YPALLLVVGGAVMLFLLAFVVPRFAQVFETVGGELPWMSRVLLDWGRLVQENGALLGA
                     AFLFAVCAAVALVRRPETRAAAGRLLWRLPRIGERLRIYELARFYRTLGMLLRGGIPA
                     VPAMGMVSGLLSPGLRPGLMRAIAGVREGQAFAATLQAQGLATPVALRMLAVGERAGN
                     LGEMLERAAEFHEEESARWLDVLTRLIGPLLMLVIAVAVGLILIMLYLPIFQVAESIQ
                     "
     gene            complement(3090..>3488)
                     /gene="gspG"
                     /locus_tag="PLH85_16700"
     CDS             complement(3090..>3488)
                     /gene="gspG"
                     /locus_tag="PLH85_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNB66258.1"
                     /translation="AGFTLLELLVVMTIIGLLAGYVGPKFFAHIGKSEVKAARAQIDG
                     FEKALDQFRLDMGRYPSTEEGLAALMNKPAGDAGKWAGPYLKKAVPNDPWGKPYQYVQ
                     PGEHGEFDLYSLGKDGQPGGTGEAADVVNW"
BASE COUNT          519 a         1240 c         1242 g          487 t
ORIGIN      
        1 gtgaaggccg cccgctccgc cgcgcgcagg ccggccggcc agtcgatgcg caggtagcgg
       61 gcatggcgat cggccaccgt gacgtgcagg cgtgcgcgcc gcggcaacac gggcagcgtc
      121 gccaggccca ccgcccagtc ggcggcgagg ggcgactcgg atgcggcgcc cccggcccgc
      181 gtcaggcgca ggcaatcggg cccaatgtgg agccgtatgg actcaggcca caaaggtgac
      241 acggttcagc tcctggagcg tggtgaggcc ctcgcggacc cgttgcaggc cggcctcgcg
      301 caggctgaca aagccgcgcc ggcgggcggt gtccttgagc tggcgcaggg gcgcccgggc
      361 gatgatgagg tcgcgcagtt cgtcgtccag caggagcacc tcggcgatgg ccttgcgccc
      421 gcgatagccg gtaccgcggc actggccgca gccggatccg gcacggaagg cgaagccctg
      481 cacgtcggcc ggggttgcca ggccggattc ccgcagcagg gccgcatcgg gttcgctcgc
      541 ggtgctgcag gccgggcagt tcacgcggat caggcgctgg gccaccacgc cgttgagggc
      601 cgccaccagg ttgtaggggt cgatgcccat gtgcatgaag cgcccgatca cgtcgaacac
      661 gttgttggcg tgcaccgtgg tgaacacgag gtggccggtg agggccgcct gcacggcgat
      721 ctcggcggtc tcggcgtcgc ggatctcgcc caccatgatc ttgtccgggt cgtggcgcag
      781 gatggagcgc aggccccggg cgaagctgag gcctttcttt tcgttcaccg ggatctgcag
      841 cacgccgggg agttggtatt ccaccgggtc ctcgatggtg acgatcttgt cgtggccggt
      901 gttggtctcg gagagggtcg cgtacaggct ggtggtcttg ccggaaccgg tcgggccggt
      961 gaccagcagc atgccgtagg gttcgccggc catgtggcgc agttgctggc gcacggccgg
     1021 gtcgaagccg agggtctcca gggtgaggcc ggccagttcg cgggtcaggt gttctttgtc
     1081 gaggatgcgc agcacggcgt cttcgccgtg gatgctgggc atgatggaga cgcggaagtc
     1141 gatgggccgc ccgctgacct gcaccttgaa acggccgtcc tggggcacgc ggcgctcggc
     1201 gatgtccagt tcggccatca ccttgaggcg cgacacggcc tgctcggcca gctccgtgcc
     1261 ctgcacgccg ccgacccggg cgagcacgcc gtccacccgg tatttgatga ccagcccgcc
     1321 aggcacgctt tccaggtgga cgtcgctggc gccctgcttg atggcgtcgt agagcgtgga
     1381 gttgaccagc ttgaccaccg ggctggcgtc cccggcgatg cgccgcaggg agaggtcctc
     1441 gccggcgtcg ccggtggcgg cctcctcgcg gccggccagc atgccggcca ccacccgccg
     1501 gctctcttcg tggcgcgcga ggcaggcggt caggtcgtcc cggtctgcca ggcggacgac
     1561 gaagggatgg agcaggcgcg cgcccaggcc gtccaccaga tcggtatcga aggggtcggc
     1621 caccgccagc accgtgtgac cgcccgcgtc ccgcccgagc agggcctcgc ggcgcagggc
     1681 ctcgtcgaag ctcaatccgt cgaaatccgg tgccagcgcg aacagctcgg ctgccgtcag
     1741 gcgcgtcagc ccgaccgtgg ccgccaggcg tgccgggaag tcatcgctcg cggctgcacc
     1801 gtccggtcgc gccagcacct ccaccaggcg cacgccgtcc tgtccggccc ggcgcgccgc
     1861 ggcggccagt tcggcggcgg agaagggcat cgctgacgcg ctcactggat gctctccgcc
     1921 acttggaaga tgggcaggta cagcatgatc aggatcaggc ccaccgccac cgcgatgacc
     1981 agcatcagca gcgggccgat caggcgggtc agcacgtcca gccagcgggc gctctcctcc
     2041 tcgtggaatt cggcggcccg ttcgagcatc tcgcccaggt tgccggcgcg ctcgcccacg
     2101 gccagcatgc gcagggccac cggcgtggcc aggccctggg cctgcagcgt cgcggcaaag
     2161 gcctgccctt cgcgcacgcc tgcgatggcc cgcatcagac cgggccgcag tcccggcgac
     2221 agcaggccgc tcaccatgcc catcgccggc accgccggga tgccgccacg caggagcatg
     2281 cccagggtcc ggtaaaagcg cgccaactca tagatgcgca ggcgctcgcc gatgcgcggc
     2341 aggcgccaca gcaggcgtcc ggccgccgcg cgggtctcgg gccggcgcac cagcgccacc
     2401 gcggcacaca cggcaaacag gaacgcggcg cccagcagcg cgccgttctc ctgcaccagc
     2461 cggccccaat ccagcagcac gcgggacatc cacggcagtt cgccgccgac ggtctcgaag
     2521 acctgggcga aacgcggcac cacgaaggcc agcaggaaga gcatcaccgc cccaccgacc
     2581 accagcagca gcgccggata caccgccgcc gacacgagct tgctgcgcac cagctcgagc
     2641 tggcgctgat aatcgatgta gcgggccagc gccggctcca ggtcgctggt gcgctcggcg
     2701 gcccgcaggg tggccacgaa gaggtcggga aaggcctggg gctggctttc gaggcaggcg
     2761 gaaaaggtgc gcccttcccg caatccggag cccagcgcag tcagcacggc ctgcaccgcg
     2821 gggcgcgatt ccttctcggc cagggcggcg atcccgtcgg cgaggggaat gcccgccttc
     2881 aacagggcca gcagctcctg gctgaagagc atcagtggaa aacccgtccg ccgcccgccg
     2941 ccgccccgca gcgcggtggc ggacagcacg gcgagaccgc gcccggccag cagcgtccgc
     3001 gcggacgcct cgtcgacggc ctccacctcg agctcctgca cggccgcgcc gtttcccacg
     3061 gccttgatcc ggaaacgcat cgacgcggcc taccagttga ccacgtccgc cgcctcgccg
     3121 gtaccgcccg gctggccgtc cttgcctaag gagtacaggt cgaattcacc gtgttcgccc
     3181 ggctgcacat attggtaggg cttgccccag gggtcgttgg gcacggcttt cttgagatag
     3241 ggtccggccc atttgccggc gtcgccggcg ggcttgttca tcagcgcggc cagtccctcc
     3301 tcggtgctcg ggtagcgtcc catgtcgagg cggaactggt cgagggcctt ctcgaagccg
     3361 tcgatctggg cgcgggcggc cttgacctcc gacttgccga tgtgggcgaa gaacttgggg
     3421 cccacatagc ccgccaggag gccgatgatg gtcatcacca ccagcagttc cagcagcgtg
     3481 aaccccgc
//
LOCUS       DAOQMM010000171         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_18983_length_3415_cov_52.431845, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000171 DAOQMM010000000
VERSION     DAOQMM010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18983_length_3415_cov_52.431845"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="PLH85_16705"
     CDS             <1..1384
                     /locus_tag="PLH85_16705"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNB66259.1"
                     /translation="DMFKEHRCVDFGMDQAEKSPGDGVIVGYGTVNGRLVFVFSQDFT
                     VFGGSLSETHAEKICKVMDHAMKVGAPVIGINDSGGARIQEGVASLGGYADVFQRNVM
                     ASGVVPQISMIMGPCAGGAVYSPSMTDFIFMVKDSSYMFVTGPEVVKTVTHEDVTAEE
                     LGGAVTHTSKSGVADLAFENDVEALSMLRRFMNFLPANNREKPPVQSTNDPVDRMDYS
                     LDTLVPDNANKPYDMKELLVKVVDDSDFFELQADYAKNIIIGFGRIDGHPVGIVANQP
                     LVLAGCLDIKSSIKAARFVRFCDAFNIPVVTFVDVPGFMPGTAQEYGGIIKHGAKLLY
                     AFAECTVPKVTVITRKAYGGAYDVMSSKHLRGDVNLAWPSAEIAVMGPKGAVEIIFRE
                     EKNDPAKLAQREAEYKAKFANPFVAGARGFIDDVIMPHATRKRIARSLAMLRDKKLEN
                     PWRKHGNIPL"
     gene            1404..3407
                     /gene="accC"
                     /locus_tag="PLH85_16710"
     CDS             1404..3407
                     /gene="accC"
                     /locus_tag="PLH85_16710"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB66260.1"
                     /translation="MFTKILIANRGEIACRVIKTARKMGIQTVAVYSEADRDALHVEL
                     ADEAVCIGPAASKESYLVMDKIIAACKQTGAQAVHPGYGFLSENAEFSRRLEEEGIKF
                     IGPKHYSVAKMGDKIESKKLAIAAQVNTIPGYNDAIAGPAEAVEIAKKIGYPVMIKAS
                     AGGGGKGLRVAYNDAEAFEGFSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDAH
                     GNYLYLNERDCSIQRRHQKVIEEAPSPFVDAEMRKAMGEQAVALARAVNYESAGTVEF
                     VVSGATKEFYFLEMNTRLQVEHPVTELITGLDLVEQMIRVAYGEKLPISQADVGINGW
                     AMECRINAEDPFRGFLPSTGRLVKFQPPADVDGQVRVDTGVYDGGEISMYYDSMIAKL
                     IVHGATRDQAIARMRDALNGFVIRGISSNIPFQAALMQHPRFQSGIFDTGFIAKEYPK
                     GFDASMVPHDDPALLVSVAAYVYRAYTDRSASVSGQLQGHERIVGDHWMVIRLSKDGN
                     EQHPVVARPIDGGYHVEYKGESYELKSDWKLGESLFNGTCNGEPFTLQVERNRTRYSL
                     YHWGTRADFMVMSGRAAELLALMPEKLPPDLSKFLLSPMPGLLREVAVKVGQDVKAGE
                     KLAVIEAMKMENILKAEQDCKVKSIVAAAGASLAVDEVIIEFE"
BASE COUNT          668 a         1249 c          955 g          543 t
ORIGIN      
        1 ggacatgttc aaggagcacc gctgcgtcga cttcggcatg gaccaggccg aaaagtcccc
       61 cggtgacggc gtcatcgtcg gttacggcac cgtcaatggc cgcctggtgt tcgtgttcag
      121 ccaggacttc accgtgttcg gcggctccct gtccgagacc cacgccgaga agatctgcaa
      181 ggtcatggac cacgccatga aggtcggcgc cccggtcatc ggcatcaatg attccggcgg
      241 cgcccgcatc caggaaggcg tcgcctccct cggcggctac gccgacgtgt tccagcgcaa
      301 cgtgatggcc tccggcgtcg tgccccagat ctccatgatc atgggcccct gtgccggcgg
      361 tgccgtgtat tccccgtcca tgaccgactt catcttcatg gtcaaggact cctcctacat
      421 gttcgtgacc ggtcccgaag tcgtgaagac cgtgacccac gaagacgtca ccgccgagga
      481 gctgggcggc gccgtcaccc acaccagcaa gtccggcgtg gccgacctgg ccttcgagaa
      541 cgacgtggaa gccctctcca tgctgcgtcg tttcatgaac ttcctgcccg ccaacaaccg
      601 cgagaagccg cccgtgcaat cgaccaacga cccggtcgac cgcatggact actccctcga
      661 caccctggtg ccggacaacg ccaacaagcc ctacgacatg aaggaactgc tcgtcaaagt
      721 ggtggacgac agcgacttct tcgagcttca ggccgactac gccaagaaca tcatcatcgg
      781 ctttggccgc atcgacggtc acccggtggg catcgtcgcc aaccagcccc tggtgctggc
      841 cggctgcctg gacatcaagt cctccatcaa ggccgcccgc ttcgtgcgct tctgcgacgc
      901 cttcaacatc cccgtcgtca cctttgtcga cgtgcccggc ttcatgcccg gcaccgccca
      961 ggaatacggc ggcatcatca agcacggcgc caagctgctc tacgcctttg ccgaatgtac
     1021 cgtgcccaag gtcaccgtca tcacccgcaa ggcctacggc ggcgcctatg acgtgatgtc
     1081 ctccaagcac ctgcgcggcg acgtcaacct cgcctggccc tccgccgaaa tcgcggtgat
     1141 gggccccaag ggcgcggtgg agatcatctt ccgcgaagaa aagaacgacc ccgccaagct
     1201 cgcccagcgc gaagccgagt acaaggccaa gttcgccaac cccttcgtgg ccggcgcccg
     1261 cggcttcatc gacgacgtga tcatgccgca cgccacccgc aagcgcatcg cccgctcgct
     1321 ggccatgctg cgcgacaaga aactggaaaa tccgtggcgc aagcacggca acatcccgct
     1381 ctgatcgccg aggacatcaa gacatgttta ccaagatcct gattgcgaac cgcggcgaga
     1441 ttgcttgccg cgtcatcaag accgcccgca agatgggcat ccagaccgtc gccgtgtatt
     1501 ccgaggccga ccgggacgcc ctgcacgtgg agctggccga cgaagccgtg tgcatcggcc
     1561 cggccgcctc caaggaatcc tacctggtca tggacaagat catcgccgcg tgcaagcaga
     1621 ccggcgccca ggctgtccac cccggctacg gcttcctctc cgagaacgcg gaattctccc
     1681 gccgcctcga agaagaaggc atcaagttca tcggccccaa gcactactcc gtggccaaga
     1741 tgggcgacaa gatcgagtcc aagaagctcg ccatcgccgc ccaggtcaac accatccccg
     1801 gctacaacga cgccattgcc ggcccggccg aagccgtcga gatcgccaag aagatcggct
     1861 acccggtgat gatcaaggcc tccgccggcg gcggcggcaa gggtctgcgc gtggcctaca
     1921 acgacgccga agccttcgaa ggcttctcct cctgcgtcaa tgaagcccgc aactccttcg
     1981 gcgacgaccg cgtcttcatc gaaaaatacg tcctcgaacc gcgccacatc gaaatccagg
     2041 tgctgggcga cgcccacggc aattacctgt acctcaacga gcgcgactgc tccatccagc
     2101 gccgccacca gaaggtcatc gaagaagcgc cgagcccctt cgtggacgcc gagatgcgca
     2161 aggccatggg cgaacaggcc gtggccctgg cccgcgcggt gaactacgaa tccgcgggca
     2221 ccgtcgaatt cgtcgtctcc ggcgccacca aggagttcta cttcctcgag atgaacaccc
     2281 gtctccaggt ggaacacccg gtcaccgaac tcatcaccgg cctcgacctc gtcgagcaga
     2341 tgatccgcgt cgcctacggc gaaaagctcc ccatcagcca ggccgacgtg ggcatcaacg
     2401 gctgggccat ggagtgccgc atcaacgccg aagacccctt ccgcggcttc ctcccctcca
     2461 ccggtcgtct ggtcaagttc cagccgcccg ccgatgtgga cggccaggtg cgtgtggata
     2521 ccggcgtgta cgacggtggc gagatctcca tgtactacga ctcgatgatc gccaagctca
     2581 tcgtccacgg cgccacccgc gaccaggcca ttgcccgcat gcgcgacgcc ctcaacggct
     2641 tcgtgatccg cggcatctcc tcgaacatcc cgttccaggc cgcgctcatg cagcacccgc
     2701 gcttccagtc cggcatcttc gacaccggct tcatcgccaa ggaatacccc aagggcttcg
     2761 acgcctccat ggtgccccac gatgatcccg ccctgctggt ctccgtcgcc gcctacgtgt
     2821 accgcgccta caccgaccgc tccgcctccg tgagcggcca gctgcaaggc cacgaacgca
     2881 tcgtcggcga ccactggatg gtcatccgcc tcagcaagga cggcaacgag cagcaccccg
     2941 tcgtcgcccg tcccatcgac ggcggctacc acgtggaata caagggcgag agctacgagc
     3001 tcaagtccga ctggaagctc ggcgagtccc tcttcaacgg cacctgcaac ggcgaaccct
     3061 tcaccctcca ggtggagcgc aaccgcaccc gctacagcct ctaccactgg ggcacccgcg
     3121 ccgacttcat ggtcatgagc ggccgtgccg ccgagctgct ggccctcatg cccgagaagc
     3181 tgccgcccga cctctccaag ttcctgctgt cccccatgcc cggcctgctg cgcgaagtgg
     3241 ccgtcaaggt cggccaggac gtgaaggccg gcgaaaagct cgccgtcatc gaagccatga
     3301 agatggaaaa catcctcaag gccgagcagg actgcaaggt caagtccatc gtcgccgccg
     3361 ccggcgccag cctcgcggtg gacgaagtca tcatcgagtt cgagtaatcg gctcc
//
LOCUS       DAOQMM010000172         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_19321_length_3375_cov_43.691566, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000172 DAOQMM010000000
VERSION     DAOQMM010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19321_length_3375_cov_43.691566"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="aceE"
                     /locus_tag="PLH85_16715"
     CDS             complement(<1..916)
                     /gene="aceE"
                     /locus_tag="PLH85_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990311.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNB66261.1"
                     /translation="MAANTQVVLQSDIDAQETREWLEALEGVVTQEGPERAHFLIEKL
                     IEAGREEGIDIPYSANTQYINTIPADQQPRYPGDPDMEIKLHSYIRWNAMAMVVRANK
                     HTNVGGHIASFASAAALYDVGFSHFWKSIDHDTGGDLIYFQGHSVPGVYSRAYMLGRL
                     TDEQMDNFRQESDGQGISSYPHPWLMPDFWQFPTVSMGLGPICAIYSARFMKYLASRG
                     LIDAAKAEQRKVWAFLGDGETDEVESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRG
                     NGKIIQELEAEFRGAGWNVIKLIWGTHWD"
     gene            complement(1309..2484)
                     /locus_tag="PLH85_16720"
     CDS             complement(1309..2484)
                     /locus_tag="PLH85_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB66262.1"
                     /translation="MGVGLGLALAGAGAGAVEPIKIGVTGPFTGGSSPMGVAMRDGIR
                     LAAQELNEKGGILGRPLALVERDDEARPERGAQVVQELINHEKVVAGLGIVNTGVALA
                     SQRFYQQAHLPMITSVATGTIVTRQFLPPQYPDNYIFRVSAPDSVQATMIVEEAVERR
                     RFRKLAILHDSTNYGQLGREDLERALERHGIRPVAVERFNIRDVDMTAQLTRARAAGA
                     EAILTYGLGPELAHIANGTVRIGWKVPLIGSWALAMSNFIDNAGPNAEGTRMPQTFIQ
                     EGDTPRRKDFIDRYRKAFGVERIPVAPAAAQGYDSMLVLAAAIRQARSTDGPKVREAL
                     ETLKERVEGIVMVYDRPFSRQDHEAIGSTRIPVIGEVRNSQVVFAYDEDRKRGAQHK"
     gene            2736..>3375
                     /locus_tag="PLH85_16725"
     CDS             2736..>3375
                     /locus_tag="PLH85_16725"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNB66263.1"
                     /translation="MVKLYVSYAWSAEGSDDAGVVSRLEAVTQGSDVELVRDIRRIGY
                     GQSIRSFMDEIGAADQVVLVLSEAYFRSPYCMYELRAVHGHADFRRRVHPVVLKGCAL
                     HDPLWWVDILEHWEVQAHILEGKLGKISRAHTKRLHETLDDYVDFRRLLDGQMNTLAD
                     MNALTEGLHRDTDFAALLARVRGNEPALAVERPAAQPAAQPQPQPQPQPQPQP"
BASE COUNT          522 a         1095 c         1186 g          572 t
ORIGIN      
        1 cgtcccagtg ggtgccccag atcagcttga tgacgttcca gccggcgccg cggaactcgg
       61 cttcgagctc ctggatgatc ttgccgttgc cgcgcaccgg gccgtccagg cgctgcaggt
      121 tgcagttgat gacgaagatc aggttgtcga gcttttcgcg gccggccatg ccgatggcgc
      181 cgagggactc gacttcgtcg gtctcgccgt cgccgaggaa ggcccacacc ttgcgctgct
      241 cggccttggc ggcgtcgatg aggccgcggc tggcgaggta cttcatgaag cgcgcggagt
      301 agatcgcgca gatcgggccg aggcccatgg agacggtggg gaactgccag aagtccggca
      361 tcagccaggg gtgcgggtag gacgagatgc cctggccgtc ggattcctgg cggaagttgt
      421 ccatctgctc gtcggtgagg cggccgagca tgtaggcgcg ggaatacacg ccgggcaccg
      481 agtggccctg gaagtagatc aggtcgccgc cggtgtcgtg gtcgatgctc ttccagaagt
      541 gggagaagcc cacgtcgtag agggccgcgg cagaggcgaa ggaggcgatg tggccgccca
      601 cgttggtgtg cttgttggcg cgcaccacca tggccatggc gttccagcgg atgtaggagt
      661 ggagcttgat ctccatgtcc gggtcgccgg gataacgggg ctgctggtcg gccgggatgg
      721 tgttgatgta ctgggtgttg gcggagtagg ggatgtcgat cccttcctca cggccggctt
      781 cgatgagctt ctcgatcagg aagtgagccc gttcggggcc ttcctgggtc acgacgccct
      841 cgagcgcctc cagccattcc cgggtttcct gggcgtcgat gtccgactgc aggacgactt
      901 gcgtgttcgc ggccatggat tttgtctcct catgacttgc gggttgtgga tggccgccgt
      961 gggtaacggc ggcggatttt ttcactgcaa acctgcgggg ctggatcaca gcccgttttt
     1021 cgtaatataa aaccaaatct cgcgatgtgc aataagggtc aaccgcaatg ggggtgatga
     1081 cgccccgtgc gacgcccgca cgaacgccac cgcgcggccc cgaggacggg caggcgtgta
     1141 gacgccggcg ggcccgccgt ggggtcgtgc ggtgactgcc ggccggcgct tgacgcgcac
     1201 ggccggcggc ccttcattcg atagggaggg aaaaagacgg ccgggggaga gggagggagg
     1261 gagagaaccc ccggccggtt gcggaaaatg gctgatccgc gggcggggtc atttgtgctg
     1321 cgcgccccgc ttgcgatcct cgtcatacgc gaagaccact tggctattgc gaacttctcc
     1381 gatcacgggg atccgcgtgc tgccgatcgc ttcgtgatcc tgcctggaga aggggcgatc
     1441 atataccatc acgatgccct cgacgcgctc cttcagcgtt tccagcgcct cgcgcacctt
     1501 gggtccgtcg gtgctgcggg cctgtcggat ggcggcggcg agcaccagca tggagtcgta
     1561 gccctgggcc gcggcgggcg ccaccgggat gcgctccacg ccgaaggcct tgcggtagcg
     1621 gtcgatgaaa tccttgcgac gcggcgtgtc cccctcctgg atgaaggtct ggggcatgcg
     1681 cgtcccctcc gcgttggggc ccgcgttgtc gatgaagttg gacatggcca gcgcccagct
     1741 gccgatcagc ggcaccttcc agccgatgcg caccgtgccg ttggcaatgt gggccagctc
     1801 cggccccagg ccgtaggtga ggatcgcctc tgccccggcg gcgcgggcgc gggtgagctg
     1861 ggcggtcatg tccacgtcgc ggatgttgaa gcgctccacc gccaccgggc gaatgccgtg
     1921 ccgctccagc gcccgctcca ggtcctcgcg gccgagctgg ccgtagttgg tgctgtcgtg
     1981 gaggatcgcc agcttgcgga acctgcgccg ctccaccgcc tcctcgacga tcatcgtggc
     2041 ctgcacgctg tccggcgccg acacgcggaa gatgtaattg tccggatact ggggcggcaa
     2101 aaattgccgg gtgacgatgg tcccggtggc cacggaggtg atcatcggca ggtgcgcctg
     2161 ctgatagaag cgctggctcg ccagggccac gccggtgttc acgatgccca gcccggccac
     2221 caccttctcg tggttgatga gctcctgcac cacctgggcg ccgcgctccg ggcgcgcctc
     2281 gtcgtcccgc tccaccagcg ccagcgggcg cccgaggatg ccgcccttct cgttgagctc
     2341 ctgggccgcc aggcggatgc cgtcgcgcat tgctacgccc atcgggctcg acccgccggt
     2401 aaacgggccg gtcacgccga tcttgatcgg ctccaccgcg cccgccccgg cgccggccag
     2461 ggccagcccg aggcccacgc ccacacgccg cccccaggcc cccagcctcc gcaggaatcc
     2521 cgtcacgccc gtcctccccg ttggccgccg cacgcccatc cgcgccggcc cggcccccgc
     2581 acgccaccct agcgcggcgc ggggagcggg ggaatacgcg attaccctta gcgctgaagg
     2641 gccgcgaaga acggtgaacg gcggaaaccc ttcaccttgc cgccgaccgc gcccatcggc
     2701 gatgatccgg gcatgcatgc acaagggaga acggggtggt gaagctctac gtgtcctacg
     2761 cctggtcggc ggaaggcagc gacgacgccg gcgtggtgag ccggctggag gcggtgaccc
     2821 agggttcgga cgtggaactg gtgcgggaca tccgcagaat cggctacggg cagtccatcc
     2881 gcagcttcat ggacgagatc ggcgcggccg accaggtggt gctggtcctc agcgaggcct
     2941 acttccgttc gccctactgc atgtacgagc tgcgggcggt gcatgggcac gcggatttcc
     3001 ggcggcgggt gcatccggtg gtgctcaagg gctgcgcttt gcatgacccc ctgtggtggg
     3061 tcgacatcct ggaacactgg gaagtgcagg cgcacatact ggagggcaag ctcgggaaga
     3121 tcagtcgcgc ccacaccaag cgtctgcacg aaacgctgga tgattacgtg gatttccggc
     3181 ggctgctgga tggtcagatg aacaccctgg cggacatgaa cgccttgact gaaggtctcc
     3241 accgggacac cgacttcgca gccctgctgg cgcgggtgcg tgggaacgag ccagcgcttg
     3301 cggttgagcg cccagcggct cagcctgctg cgcaaccgca accgcaaccg caaccgcaac
     3361 cgcaaccgca accgc
//
LOCUS       DAOQMM010000173         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_20981_length_3218_cov_40.493203, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000173 DAOQMM010000000
VERSION     DAOQMM010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20981_length_3218_cov_40.493203"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="PLH85_16730"
     CDS             <1..1753
                     /locus_tag="PLH85_16730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB66264.1"
                     /translation="TKPAVPPTPTPPAPAGTAAAAGAPVADPNAPQTPPPPPLSDVIF
                     TNGDVITLDDKRPTAQAVAIKGSKILAVGKTKDILARWKSEGTEVVDLAGKTLAPGFI
                     DAWGGLSRLGLNSVAAQIQPPPAGTAMDVAAIQRTLRDWAKGDIARRFGWIVGFGYDD
                     SLLREQRPLSRADLDAVARDTPVLIIHASGRQVVLNSRALELAGITRDSTAPAGGAIG
                     RWPGTKEPDGVLDGAAAVALLGQLPQLPPDERQALIQQGQTLYLRNGYTTAVEARATP
                     EDLALYTQAADTGALKLDVAIYADLAASGAVLKGHKSVGPRYYKNRLRLAGVSMALDG
                     SAEDRSAWLTDAYRLPPPGKRAGFAGYGFATDEDVTALATRATANGWALAVQANGDAA
                     IDQFLRALRAATPAPGKGLRPLLAGAQTLRDDQLDALQVLGVGVSLAPQRLALSRPVL
                     VDDTLGTERTAHFAPAAGVLARGLPLTLHTDPALIEPAPLAVMAAALKRPVGADQQIA
                     AVDAFKALTLTAARQIGEDKTKGSVVAGKLADLVVLSANPLKTPPERLGDIQVIGTVK
                     EGVTVFGGADGGVPITR"
     gene            complement(1945..2613)
                     /locus_tag="PLH85_16735"
     CDS             complement(1945..2613)
                     /locus_tag="PLH85_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB66265.1"
                     /translation="MSTVNAASWGARACLITLGLLLGTVAQSVSAQTLPPSPRDHLER
                     IVGGKPGERKAAEDAGRKASFFCANCHGETGNSKYSEVPNLASQHPAYVLNQIEAFLS
                     GKRKDAFMQGLMKVLSAEEKADIAVYFAAQTVIPSARPGARAAEGRTLFQQNCARCHG
                     PEAKGGETFPRLAGQQPEYLRRSLTRYLKQTGERIYPPMTAAVTGLGERNIDAVVDFL
                     SSLN"
     gene            2749..>3218
                     /locus_tag="PLH85_16740"
     CDS             2749..>3218
                     /locus_tag="PLH85_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB66266.1"
                     /translation="MSPMSAPPAPPSRPCVAIVGGGPAGLMAAETLARGGVAVEVFDA
                     MPSVGRKFLLAGRGGLNITHSEPPERFLTRYGARQGQIAPLLDAFGPEALRAWVEALG
                     IATFTGSSGRVFPEGMKAAPLLRAWLQRLREAGVRVHVRHRWTGWDADGALRFAT"
BASE COUNT          434 a         1169 c         1178 g          437 t
ORIGIN      
        1 gaccaagccc gcagtgccgc cgacgccgac gccccccgcg ccggccggca cggccgctgc
       61 tgcgggcgcg cccgtggccg atcccaacgc gccgcagacg ccgcctccgc cgcccctgtc
      121 ggacgtgatc ttcaccaacg gcgacgtgat caccctggac gacaagcgcc ccacggctca
      181 ggcggtggcg atcaagggca gcaagatcct ggccgtgggc aagaccaagg acatcctggc
      241 caggtggaag agcgagggca ccgaggtggt ggacctggcg ggcaagaccc tggcgccggg
      301 cttcatcgac gcctggggcg ggctgtcgcg cctggggctc aattctgtgg cggcccagat
      361 ccagccgccg ccggccggca cggcgatgga cgtggcggcc atccagcgca cccttcggga
      421 ctgggccaag ggcgacatcg cccggcgctt cggctggatc gtcggcttcg gctacgacga
      481 cagcctgctg cgcgaacagc gtcccctgtc acgggcggat ctggacgccg tcgcccgcga
      541 cacgccggtg ctgatcatcc acgcctccgg ccgccaggtg gtgctcaaca gccgcgcgct
      601 ggagctggcc ggcatcaccc gcgacagcac cgcgccggca ggtggggcca tcggccgctg
      661 gccgggcacc aaggagccgg acggggtgct ggacggcgct gccgcggtgg cgctattggg
      721 gcagctgccg cagctgccgc cggacgaacg ccaggccctg atccagcaag gccagaccct
      781 gtatctgcgc aacggctaca ccaccgccgt ggaagcccgc gccacgccgg aggatctggc
      841 cctctacacc caggccgccg acaccggcgc cctcaagctg gacgtggcga tctatgccga
      901 tctggccgcg agcggcgccg tgctcaaggg ccacaagagt gtggggccca ggtactacaa
      961 gaaccgcctg cgcctggccg gcgtgtccat ggccctggac ggcagcgccg aggaccgcag
     1021 cgcctggctc acggacgcct accgcctgcc cccgccgggc aagcgcgccg gctttgcggg
     1081 ctacggcttc gccaccgacg aggacgtcac ggccctcgcg acccgcgcca cggccaacgg
     1141 ctgggcgctg gcggtgcagg ccaacggcga cgcggccatc gaccagttcc tgcgcgccct
     1201 gcgggccgcc actccggcgc cgggcaaggg cctgcgtccc ctgctggctg gcgcccagac
     1261 gctccgcgac gatcagctcg acgcgctcca ggtactgggc gtcggcgtga gcctggcgcc
     1321 ccagcgcctc gccctgtccc gtccggtgct ggtcgacgac accctcggca cggagcgcac
     1381 cgcgcacttc gcccccgcgg ccggcgtgct cgcccgcggc ctgcccctga ccctgcacac
     1441 cgacccggcc ctgatcgaac cggcgccgct ggcggtgatg gccgccgcgc tgaagcgccc
     1501 agtcggcgcg gaccagcaga tcgccgccgt ggacgccttc aaggccctga ccctcaccgc
     1561 cgcgcggcag atcggcgagg acaagaccaa gggcagcgtg gtggccggca agctggcgga
     1621 tctggtggtg ctctccgcca atcccctcaa gacgccgccg gaacgcctgg gcgacatcca
     1681 ggtgatcggc acggtgaagg agggggtgac ggtcttcggc ggcgcggacg gcggggtgcc
     1741 gatcacgcgc tgaggcggga atggatgcga cgcggccgac cggtgacggc gggatcttcg
     1801 cgcaggtacg gaagcggcgc ggacggccgc cagcgggggg ctggggggcg cagcgcacct
     1861 gcccgaccac ctgtgcggga cgatgaccgc gatcggtcgt cccgacgcgg gcccggccgg
     1921 atcgtccgtg ccggacccgg acgctcagtt gaggctggac aggaaatcca ccaccgcgtc
     1981 gatgttgcgc tcgcccaggc cggtgacggc ggcggtcatg ggcgggtaga tacgctcgcc
     2041 ggtctgcttg aggtagcggg tcaggctgcg gcgcaggtat tccggctgct ggccggccag
     2101 gcgcgggaag gtctcgccgc ccttggcctc ggggccgtgg cagcgggcgc agttctgctg
     2161 gaagagggtg cgcccttcgg ccgcgcgggc gccgggccgg gcagagggga tcaccgtctg
     2221 ggcggcgaaa tagacggcga tgtcggcctt ctcctcggcg ctcagcacct tcatcaggcc
     2281 ctgcatgaag gcatccttgc gcttgccgga gaggaaggcc tcaatctggt tgagcacgta
     2341 ggccgggtgc tggctggcca ggttgggtac ttcggaatac ttgctgttgc cggtctcgcc
     2401 gtgacagttg gcgcagaaga aggaggcctt gcgcccggca tcctcggccg ccttgcgctc
     2461 gccgggcttg ccaccgacga tgcgctccag gtgatcccgc gggctgggcg gcagggtctg
     2521 ggcgctgacc gattgggcga cggtgcccag cagcagtccc agcgtgatca ggcaggcacg
     2581 ggcgccccag gacgccgcgt tgacggtgga catgatgtct cctcgttgga cgtgtcggga
     2641 cggacgaccc tgtccgctcc gagtgaagcg gcattgtagt cgcgccgcgg ccgtcccgca
     2701 cggcatgcgc gggacgatcc actacaattc cggttccgtc ctgcccccat gtcaccgatg
     2761 tccgctccgc ctgctccgcc ctcccgcccc tgcgtcgcca tcgtcggcgg cggccctgcc
     2821 ggcctgatgg cggccgagac cctggcccgc ggcggtgtgg cggtggaggt cttcgacgcc
     2881 atgccctcgg tggggcgcaa gttcctgctg gccgggcgcg gcgggctgaa catcacccat
     2941 tccgaaccgc cggaacgctt cctcacccgc tacggggccc gccaggggca gatcgcgccg
     3001 ctgctcgacg ccttcggtcc ggaagcgttg cgggcctggg tggaggcgct ggggattgcc
     3061 accttcaccg gcagctcggg ccgcgtcttt cccgagggca tgaaggccgc gccgctgctg
     3121 cgggcctggc tgcagcgcct gcgcgaggcc ggcgtgcggg tgcatgtgcg ccaccgctgg
     3181 accggctggg acgcggacgg cgccctgcgc tttgccac
//
LOCUS       DAOQMM010000174         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_21334_length_3184_cov_39.447427, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000174 DAOQMM010000000
VERSION     DAOQMM010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21334_length_3184_cov_39.447427"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLH85_16745"
     CDS             complement(<1..209)
                     /locus_tag="PLH85_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HNB66267.1"
                     /translation="MSDTLDRLVAEARKAADARAEGYRARALKLYPWICGRCAREFTV
                     ANLRELTVHHRDHNHDNNPPDGSNW"
     gene            complement(206..2335)
                     /gene="rocD"
                     /locus_tag="PLH85_16750"
     CDS             complement(206..2335)
                     /gene="rocD"
                     /locus_tag="PLH85_16750"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNB66268.1"
                     /translation="MNAPLAPPRPVRIVGVGSCLGAPIFGPAAGPRVLRDGGFEQRLR
                     RVGIPATWQTLMEPSAPKPPFPPMRERMVGVGALLNDLADEVAAIHDTGAIPLVLGGD
                     HVIGAGTWRGVARSLAPRGTLGLLWIDAHLDAHTPATTHSGNIHGMPIAALLGVGAEE
                     MTDIAGPNLDPAKLVYIGVRSWEPEEKAFLESRGVKIFYMPEVRARGLAAVLDEALAI
                     VRDGTAGFGVSVDLDAMDCARLPATTCQVPDGLDPQDLADALHRLRSCDDLAALEIVE
                     YVPERDRHGDGARWVAEFAAAALGPTTARLRAQEARHGARNYAPLPVVFSRGEGAWLW
                     DVEGRRYLDMMSAYSAVSFGHSHPRLVRALTEQAGRLALTSRAYSNDRLPQMLERLTA
                     SLGYDRALPVNTGLEAVETALKAARKWAYTVKGVPADQAEIIVCDGNFHGRSTTIVGF
                     SSEDQYRDGFGPFTPGFRRIPFGDADALAAALTPHTAAFLFEPIQGEGGIRIPPAGWL
                     ARCADLCRQANVLLIADEVQTGLGRTGRLLACEHEGVRPDGVILGKALGGGLLPVAAF
                     VADDAVMQVFQPGDHGSTFGGNPLSSAVADEALALLADEHLAERAATLGARFLARLQR
                     IANPLIREVRGRGLLIGLELDTRRVNARLAAEWLLARGLMTKDTHDSVLRFAPPLIVA
                     EADLDWAGDVVEEALADLARRVTGSTP"
     gene            complement(2465..>3184)
                     /locus_tag="PLH85_16755"
     CDS             complement(2465..>3184)
                     /locus_tag="PLH85_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNB66269.1"
                     /translation="FRDGFFHADMHPGNIFVATEGPTRGRYIALDFGIIGTLNDVDKN
                     YLATNFLAFFQRDYRRVALAHIEAGWVPPHTRVDEFEGAIRAVCEPIFDRPLKEISFG
                     KTLLRLFQTARRFEMEVQPQLVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLERWMND
                     QIGWRGLLRHVKDEAPAWAAMLPQLPRLVHRALAAPHDELARAEIARLSASNRRLSVL
                     LGIAGVLLAGGGALALWQHYH"
BASE COUNT          420 a          954 c         1348 g          462 t
ORIGIN      
        1 tcccagttgc tgccgtccgg cgggttgttg tcgtggttgt ggtcgcggtg gtggacggtg
       61 agttcgcgca ggttggcgac ggtgaattcc cgcgcgcagc ggccgcagat ccacgggtag
      121 agcttgaggg cgcgggcgcg gtagccttcg gcgcgggcgt cggcggcctt gcgggcctcg
      181 gcgacgaggc ggtcgagtgt gtcgctcatg gggtgctccc ggtgacgcgt cgggccagat
      241 cggcgagggc ttcttccacc acgtcgccgg cccagtccag atcggcttcg gcgacgatca
      301 gcggcggggc gaagcgcagc acgctgtcgt gggtgtcctt ggtcatcagg ccgcgggcga
      361 gcagccattc ggcggcgagg cgggcgttga cccggcgggt gtccagctcc agcccgatga
      421 gcaggccgcg gccgcgcacc tcgcggatga gggggttggc gatgcgctgc aggcgggcca
      481 ggaagcgggc gccgagggtg gcggcccgtt cggcgaggtg ttcgtccgcg agcagggcca
      541 gggcttcgtc ggcgacggcg ctggagaggg ggttgccgcc gaaggtggag ccatggtcgc
      601 cgggctggaa gacctgcatc accgcgtcgt cggccacgaa ggcggccacc ggcaggagac
      661 cgccgccgag ggccttgccg aggatcacgc cgtcggggcg cacgccttcg tgctcgcagg
      721 cgaggaggcg gccagtgcgg ccgaggccgg tctgcacttc gtcggcgatg agcaggacgt
      781 tcgcctggcg gcacagatcg gcgcagcggg cgagccagcc ggccggcggg atgcggatgc
      841 cgccttcgcc ctggatgggt tcgaagagga aggcggcggt gtggggcgtg agggcggcgg
      901 cgagggcgtc ggcgtcgccg aaggggatgc gccggaagcc cggcgtgaag gggccgaagc
      961 cgtcccggta ctggtcttcg gacgagaagc cgacgatggt ggtggagcgg ccgtggaagt
     1021 tgccgtcgca gacgatgatc tcggcctggt cggcgggcac gcccttgacg gtgtaggccc
     1081 acttgcgggc ggccttgagg gcggtctcga cggcttccag gccggtgttg acgggcaggg
     1141 cgcggtcgta gccgaggctg gcggtgaggc gctccagcat ctgcggcagg cggtcgttgg
     1201 agtaggcgcg ggaggtcagc gccaggcggc cggcctgctc ggtgagggcg cgcaccaggc
     1261 gcgggtggct gtggccgaag ctcaccgccg agtaggcgct catcatgtcc aggtagcggc
     1321 ggccttccac gtcccacagc caggcgcctt caccgcgact gaagaccacc ggcaggggcg
     1381 cgtagttgcg cgcgccgtgg cgggcttcct gggcccgcag gcgggcggtg gtggggccca
     1441 gggcggcggc ggcgaactcg gccacccagc gggcgccatc gccgtggcgg tcgcgctcgg
     1501 gcacgtattc cacgatctcc agggcggcca gatcgtcgca gctgcgcagg cggtgcaggg
     1561 cgtcggccag atcctgcggg tcgaggccgt cgggcacctg gcaggtggtg gcgggcaggc
     1621 gggcgcagtc catggcgtcc agatccaccg acacgccgaa gccggcggtg ccgtcacgga
     1681 cgatggcgag ggcttcgtcg agcaccgcgg cgaggccgcg ggcgcgcact tcgggcatgt
     1741 agaagatttt gacgccgcgg ctttcgagga aggctttctc ttcgggctcc caggagcgca
     1801 cgccgatgta caccagcttg gccgggtcca ggttcgggcc ggcgatgtcg gtcatctctt
     1861 cggcgcccac gcccagcagg gcggcgatgg gcatgccgtg gatgttgccg ctgtgggtgg
     1921 tggcgggggt gtgggcgtcg aggtgggcgt cgatccacag caggccgagg gtgccgcggg
     1981 gggcgaggct gcgcgccacg ccgcgccagg tgccggcgcc gatgacgtgg tcgccgccga
     2041 ggaccagcgg aatcgcgccg gtgtcgtgga tggcggccac ttcgtcggcg aggtcgttga
     2101 ggagggcgcc gacgccgacc atgcgttccc gcatgggcgg aaagggcggc ttgggcgcgg
     2161 agggttccat cagggtctgc caggtggcgg ggatgccgac gcggcgcagg cgctgttcga
     2221 agccgccgtc gcgcagcacg cgggggccgg cggcgggacc gaagatgggg gcgccgaggc
     2281 aggacccgac gccgacgatg cggaccgggc gcggaggagc gaggggggcg ttcatggcac
     2341 gtctctctgg gggcaggccg acaacccttt caggcttgga gcgtcggcag ggcggctttg
     2401 ttctggggaa ggcgggcggc ggcgtgacgg aatgggcagt cgacggggcc ggattgcccc
     2461 ggcctcagtg gtaatgctgc cagagcgcga gggcgccgcc gccggccagc agcacgccgg
     2521 cgatgcccag gagcacgctc aggcggcggt tgctggcgct gaggcgggcg atctcggcgc
     2581 gggcgagctc gtcgtggggc gccgccaggg cgcgatgcac caggcgcggc agctggggca
     2641 gcatggcggc ccaggcgggg gcttcgtcct tgacgtggcg gagcaggccg cgccagccga
     2701 tctggtcgtt catccagcgc tcgaggaagg gcttggcggt cttccacagg tccaggtccg
     2761 ggtcgagctg acggcccagg ccctcgatgt tgagcagggt cttctgcagc aggacgagct
     2821 ggggctgcac ttccatctcg aagcggcggg cggtctggaa caggcgcagc agggtcttgc
     2881 cgaaggagat ttccttgagc ggccggtcga agatgggttc gcagacggcg cggatggcgc
     2941 cttcgaattc gtccacgcgg gtgtggggtg gcacccagcc ggcctcgatg tgggccaggg
     3001 cgacgcgccg gtagtcgcgc tggaagaagg ccaggaagtt ggtggccagg tagttcttgt
     3061 ccacgtcgtt gagggtgccg atgatgccga aatccagggc gatgtaacgc ccgcgggtgg
     3121 gcccttcggt ggcgacgaag atgttgccgg ggtgcatgtc ggcgtggaag aagccgtcgc
     3181 ggaa
//
LOCUS       DAOQMM010000175         3176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_21435_length_3176_cov_54.608138, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000175 DAOQMM010000000
VERSION     DAOQMM010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21435_length_3176_cov_54.608138"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLH85_16760"
     CDS             complement(<1..718)
                     /locus_tag="PLH85_16760"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66270.1"
                     /translation="MKQSLQHLLIPLGDGVRLEVERTAESVELHSRWQTIRTEHTARF
                     GELYCLDGDAMVSTADEFMIHESLAHHVALEHTSPRRALILGGGDGGTARELLKHPCI
                     DAIVIAELDERVVARVREALPTVSAGALDNPRVTLRLGDAGETLEASRQCGERFDLIL
                     FDLTASDDPACAHLHAPPFLNACADRLMPGGRLHVQLGSPFYQPEAVAALHRRLNGVF
                     PYVRPALISVPLYGGPWLLAA"
     gene            complement(730..1494)
                     /locus_tag="PLH85_16765"
     CDS             complement(730..1494)
                     /locus_tag="PLH85_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNB66271.1"
                     /translation="MDATYLILGLGAFFAGCVDAVVGGGGLIQIPLLFTTFPTQGTAT
                     LFGTNKLASVVGTSSAAWRYARRIAVPWKVVAPAALAAGAGSWIGARAVAYFPPAALR
                     PVMLALLIAVALYTFVRKDFGVRPDTSASPSDPWRRAVFIGAGVGFYDGFFGPGTGSF
                     LIFLFVRALNWDFLRASVAAKIVNMVTNVAAIGFFVSHGEVLWRVAAVMALCNLCGAQ
                     LGSGLALRHGAPFVRRAFLLVACVLIVRFALDTFRI"
     gene            complement(1522..2352)
                     /locus_tag="PLH85_16770"
     CDS             complement(1522..2352)
                     /locus_tag="PLH85_16770"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNB66272.1"
                     /translation="MTQALFEAAADLIAQADGLIIAAGAGMGVDSGLPDFRGNQGFWR
                     AYPALAEARIAFEEIACPDAFRDAPERAWGFYGHRLNLYRRTIPHAGFAVLKRWGLRL
                     PRGAFVFTSNVDGQFQRAGFDSNQVCEVHGSIHHLQCVQGCDGRILSAEGWSPEVDEV
                     GCRLLSPLPRCSVCGDLLRPNVLMFGDWAWLPQRTQSQRVRLEQWLAVVDRPVVVEIG
                     AGSHVATVRWFSESMGVPLVRINPTEPEVPGRGVELPMEGLRALSEIDAVLAARGALR
                     "
     gene            complement(2349..3110)
                     /locus_tag="PLH85_16775"
     CDS             complement(2349..3110)
                     /locus_tag="PLH85_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB66273.1"
                     /translation="MAWEPERYLQFSDERLRPAVDLLGRVTLASPARIVDLGCGAGNV
                     TRLLQARWPDAELRGVDNSPEMLARARQALPSAQFELAAVEDWQPDAPVDLVFSNAAL
                     HWVGEHQHLFPRLMGAVAQGGVLAVQMPGNFAAPSHRLICELAESDAWRERVGRGRMG
                     AILEMDAYFRVLSPWAQTVTVWETTYWQALSGAAPVLDWLRGTTLVPYLAPMSDLERE
                     AFLAQLGARLAVAYPKDEQGRTLFPFRRVFLVAQR"
BASE COUNT          701 a         1092 c          930 g          453 t
ORIGIN      
        1 ctgcggccag caaccagggg ccgccgtaga ggggtaccga aatgagggca gggcggacat
       61 aagggaagac gccgttcaac cggcgatgca gtgccgcaac agcttctggc tgatagaacg
      121 gcgagcccag ctgaacgtgc aggcggcctc ccggcatcag acggtcggca cacgcgttca
      181 ggaagggcgg ggcgtggagg tgggcgcacg cgggatcgtc gcttgcggtg agatcgaaca
      241 gaatgaggtc gaagcgttct ccgcattgtc gggacgcctc aagcgtttcg cccgcatcgc
      301 cgaggcgcag ggtcacgcgg gggttgtcca gagcaccggc ggacacggtc ggaagcgctt
      361 cgcgcacccg ggcgacgacg cgctcgtcca gctctgcaat cacgatggcg tcgatgcatg
      421 gatgcttcag caactcgcgg gcggtgccgc cgtcaccccc gcccagaatg agggcgcgac
      481 gaggtgatgt gtgttcgagc gcgacgtgat gcgcgagact ttcatggatc atgaattcgt
      541 cggccgtgct gaccattgca tccccgtcga ggcagtacaa ctcgccaaag cgagccgtgt
      601 gctccgtgcg gatagtctgc cagcgtgaat gtagctcgac ggattcagcg gttcgctcca
      661 cctccagccg gaccccgtcg cccaatggaa tcagtaggtg ctggaggctc tgtttcacgt
      721 gaaacactcc tagatccgaa aggtgtcgag cgcaaatcga acaatcagta cgcacgcgac
      781 gagcagaaaa gcacgacgaa cgaagggcgc accatgccgc aaggcgagac cggagcccag
      841 ctgcgcgccg cacagattgc acaaggccat gacggccgcc acgcgccaca gcacctcgcc
      901 atgggagaca aagaaaccga tggctgccac attcgtcacc atgttgacga tcttggccgc
      961 taccgatgca cgaaggaagt cccagttcaa tgcgcggacg aacaggaaga ttagaaagct
     1021 ccctgtccca ggcccaaaaa aaccgtcgta gaaaccgacg cccgcgccga tgaacactgc
     1081 ccgacgccaa ggatcagacg gcgacgcact tgtatccggc ctcaccccaa aatccttcct
     1141 gacgaacgtg tagagcgcga ccgcaatgag tagcgccagc atcacgggac gcaaggccgc
     1201 cggtggaaaa taggcgacgg cacgggcacc gatccaggac cccgcaccag cggccaatgc
     1261 ggcaggagca acgaccttcc acgggacggc aattcgccgg gcatagcgcc acgccgcact
     1321 gctcgtacct acgacgctgg ccaacttgtt ggtaccgaac agggtcgccg tcccctgggt
     1381 cggaaaggtc gtgaacagta gcggaatctg gatcaagccg cctccaccga ccactgcatc
     1441 gacgcagccc gcaaagaagg cgcccaggcc cagaatcaga taggtcgcat ccattccgcg
     1501 tgtttcacgt gaaacagctg cctagcgcaa tgcgccgcgc gccgccagaa ccgcatcaat
     1561 ttcgctgagc gcgcgcaatc cctccatggg caactccacg ccccggcctg gcacctccgg
     1621 ttcggtggga ttgatacgca cgaggggcac acccatggat tcactgaacc agcgcaccgt
     1681 cgctacgtga gagcccgccc cgatctccac cacgacgggc cgatccacaa ccgccaacca
     1741 ttgctcaaga cgaacccttt gggactgcgt ccgttggggc aaccacgccc aatctccgaa
     1801 catcagcaca ttcggccgga gcagatctcc gcagaccgag cagcgcggca acggagacag
     1861 caacctgcag ccgacctcat ccacctccgg cgaccaccct tctgcggaga gaatccggcc
     1921 atcgcaaccc tgcacgcact ggaggtgatg aatggaccca tgcacctcac acacctggtt
     1981 cgaatcaaaa cccgcacgct ggaactgtcc atccacattc gacgtaaaca cgaaggcccc
     2041 ccgcggcaaa cgcagccccc accgcttcag cacagcaaat ccggcatgag gaattgtccg
     2101 tcgatacaga ttgagccgat ggccgtagaa tccccaggca cgttccggag catcccggaa
     2161 cgcatccggg cacgcgatct cctcgaaggc aatccgtgct tcggccaagg ccggataggc
     2221 ccgccagaac ccctgattac cccgaaaatc cggcaatccc gaatcgaccc ccattccagc
     2281 cccggccgcg atgatcagac catcggcctg cgcgatcagg tcggccgcag cctcgaagag
     2341 ggcctgggtc atcgttgcgc caccaggaac acacgtcgga acggaaacag cgtgcgcccc
     2401 tgttcatcct tcggataggc caccgccagg cgggcgccca gttgagccag aaatgcctca
     2461 cgttccagat ccgacatcgg tgcgagatag ggcaccagcg tcgttccgcg caaccagtcg
     2521 agtacgggcg cagcccccga cagcgcctgc caatacgtgg tctcccatac ggtcacagtc
     2581 tgagcccatg gggacagcac cctgaaatag gcgtccatct ccaggatggc tcccatcctg
     2641 ccccgaccca cccgctcacg ccacgcgtca gattcggcca gctcgcagat caatcggtgg
     2701 gacggcgcag cgaagttgcc aggcatctgc acggccagca ccccaccttg ggcgacggcg
     2761 cccatcagcc ggggaaacag gtgttgatgt tcgcccaccc aatgcaaggc tgcattggaa
     2821 aacaccagat cgacgggcgc atccggctgc cagtcctcca ccgcggcaag ctcgaactgc
     2881 gcggacggca gcgcctgtcg ggctcgtgcc agcatctcgg gcgaattgtc cacaccccgg
     2941 agttcggcat ccggccagcg cgcctggagc agacgcgtca cattgcccgc gccacacccc
     3001 agatcgacga tccgtgccgg agaggccagc gtcacccgcc cgagcagatc gaccgccggg
     3061 cgcagacgtt catcggaaaa ctgcagatat cgctccggtt cccaggccat ggtgcctccc
     3121 ccattgaagc gcccattgtc gccgaagccg gagacgcccc acaatcggga cgactg
//
LOCUS       DAOQMM010000176         3169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_21524_length_3169_cov_60.426461, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000176 DAOQMM010000000
VERSION     DAOQMM010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21524_length_3169_cov_60.426461"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..93
                     /locus_tag="PLH85_16780"
     tRNA            18..93
                     /locus_tag="PLH85_16780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Glu,seq:ttc)
     gene            684..2198
                     /locus_tag="PLH85_16785"
     CDS             684..2198
                     /locus_tag="PLH85_16785"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNB66274.1"
                     /translation="MFSLALFLLGTVVSAVAIRYGIVAAHHFGVVDKPGGHKAHDTVT
                     PFVGGIGVVCAFMCAVGVVASGSNGFPFRLDAMAAGVLIMFITGFADDLLNLHYRIRL
                     CIQAMVGVIMIFGGGVVLRDFGHLGSASVVSLGYVGIPLTLIATIGAINALNMIDGID
                     GLSGSISLVSLVLIALALHHSGNIAYFSLVMIIAGGVAGFLIYNLRYPGHRRARCFLG
                     DNGSMVLGYAFAWLLSDLCQETKGVRVITPVTTLWLFAVPLIDTLCVMMRRVWMGGSP
                     FRADRYHLHHLFQRAGFRVQDTVFILASVQLFFGAVGLAGMYAGISEIVMLAAFGVLF
                     GLCFLVILRPWRFVPALRRVHRLLGLTSVQAVGVYVGGLTETGLMTFSKALKRDFANL
                     YDFQFSVYRQLDDITEEERSFGVVELLTEDKALIPLQIGHIKGTLKREFQGSEGLVVR
                     EFVARNAGNDRRSAEKGRPAERRSRDRRSQRKPQALLRTVCEAGDVNLLSISKG"
     gene            2235..2651
                     /locus_tag="PLH85_16790"
     CDS             2235..2651
                     /locus_tag="PLH85_16790"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNB66275.1"
                     /translation="MCTGNICRSPMGEYLLRSRLAEAGKAGEIRSAGVGALVGEPAEP
                     EAIERMQALGIDLSPHRAQQASVALCRWAELVLVMEPHHREALGDIDPTVRGKTFLLG
                     YWGKFEIPDPYRRSAAAWDEAAALIDTGIGQWVARL"
     gene            2706..>3169
                     /locus_tag="PLH85_16795"
     CDS             2706..>3169
                     /locus_tag="PLH85_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66276.1"
                     /translation="MTVIYHSGTDQTHVIKDFMNIRPEGVRAPICHPTTRIRTCLLVM
                     LCAGLGACALVPGDKAYGLRDHQSAVKLPVRQEPAAEPVPDNVTIKPITAELIIEQMK
                     QSAAERPAANASQMNGRSGVGPMPASPDYKVGPGDVLTVVVWDHPELTTPAG"
BASE COUNT          534 a          908 c          994 g          733 t
ORIGIN      
        1 acgcgaatcc tcgtccggtc cccatcgtct agaggcctag gacaccgccc tttcacggcg
       61 ataaccgggg ttcgaatccc cgtggggacg ccagccgatc cggctgaatt catctcattt
      121 tcctgttttg cagtgcaatg tacggatctt gtgcgtggca ttttgccgcg ccacgtcgct
      181 cgactgtcgg taaattttac actttcctgt cggcagtctt gcgcgaaaat ttaacgcctt
      241 gaaaaacttt gctttattca tttggcctga aatttgctag atctcgccga ggaagtcttg
      301 gcatccgggg cttcgagcga cttgcacggc ttcgtgactt attgccggtt tggggcggct
      361 cgagagtggc gttccggatg tccgatgttt ggcggttttt gtgattcaaa gcctgatttt
      421 ggcaaaaatg agccatttga acggtcattt tgagccgttc aagcattttt cgggcagggc
      481 agtgctgttt agtagggcaa gcaggggacg gtgcgatgta ttgcaagcaa tgtttcctgt
      541 gaaataatgt taatcgaatc gtccgcaccg aattcgatgc ggtcgccgac agcgatacgt
      601 cggagcggat gccggaagtg tcggcgtgga aatcaatgca gcctgggcac ggcgacagcc
      661 ggaacgggcg ggggtgaagg ggaatgtttt cgctggcgct tttcctgctg ggtaccgtgg
      721 tgtcggccgt tgcgatacgc tacggtattg ttgcggcaca ccattttggg gtcgtcgata
      781 agccaggggg gcataaggcc catgacacgg tgacgccctt cgtgggtggc atcggtgtcg
      841 tgtgtgcgtt catgtgtgcc gtcggggtgg tggcctcagg cagcaacgga tttccctttc
      901 gtttggatgc tatggcggct ggtgtgctga tcatgttcat caccggcttt gccgacgatt
      961 tgctgaacct gcactaccgc atccgcttgt gcatccaggc catggtcggc gtgatcatga
     1021 ttttcggggg aggtgtggtg ctccgcgact tcgggcacct cggctccgca agcgtggtgt
     1081 ctctggggta tgtggggatt cccttgacgt tgatcgccac catcggcgcc atcaacgccc
     1141 tcaacatgat tgatggcatc gacgggttgt ccggttccat ctcgctggtc agtctggtgc
     1201 tcattgcgct ggcgcttcac cattcgggca atatcgcgta cttctcactg gtgatgatca
     1261 ttgccggtgg cgtggctggt tttctcatct acaacctgcg ctatcccggg catcgtcggg
     1321 cacgctgctt ccttggtgac aacggcagca tggtgctggg ctatgctttt gcttggctgc
     1381 tctctgacct gtgccaggaa acaaagggcg tgagggtcat cacccccgtg accactctgt
     1441 ggttgtttgc agtacccctc attgatactt tatgcgtcat gatgcgccgg gtgtggatgg
     1501 gcggctctcc cttccgcgcg gatcgctacc atcttcatca cctgtttcag cgcgccggct
     1561 tccgggtgca ggacacggta ttcatcctgg cgtcggtgca gctcttcttc ggtgcggtcg
     1621 ggcttgccgg catgtatgcc ggaatctccg agatcgtgat gctcgccgcg tttggcgtgt
     1681 tgttcgggct gtgcttcctg gtgatcctgc gcccctggcg gtttgtgccg gcgctgcgcc
     1741 gtgtgcaccg gctgttgggc ctcacctcgg tgcaggcggt gggcgtctat gtggggggac
     1801 tcaccgaaac ggggctgatg accttctcca aagcactcaa gcgagacttc gccaacctgt
     1861 atgatttcca gttttcggtt tatcgccagc tggacgacat caccgaagaa gagcgcagtt
     1921 ttggcgtcgt ggagctgctc accgaggaca aggcactgat tccgctgcag attggccaca
     1981 tcaagggcac cctcaagcgc gaattccagg gctcggaagg cttggtcgtg cgggaattcg
     2041 ttgcgcggaa cgccgggaac gaccgccgtt ccgctgaaaa aggccgtccc gccgagcggc
     2101 gcagccgtga tcggcgctct cagcgcaagc ctcaggcgct ccttcggact gtctgtgaag
     2161 cgggagacgt caacctgctg tccatcagca agggttgaag ggaggactcc gttttgtatc
     2221 aacgcatact cgtggtgtgc acgggcaata tctgtcgcag ccccatgggg gaatacctgc
     2281 tgcgcagccg gcttgctgaa gcgggcaagg cgggcgagat ccgttccgcg ggagtgggcg
     2341 ccctcgtcgg cgagcccgcc gaacccgagg ccattgagcg catgcaggcg ctcggcatcg
     2401 atctttcacc ccatcgtgcg caacaggcca gcgtggccct gtgtcggtgg gccgaactgg
     2461 tgctggtgat ggagccgcac caccgggagg ccctcggcga catcgatccg accgtgcggg
     2521 gcaagacctt tctgcttggc tactggggca agttcgagat ccccgatccc tatcgccgtt
     2581 ccgctgctgc gtgggacgaa gccgccgcgc tgatcgacac agggatcggt cagtgggttg
     2641 cccggctcta ggcccccgcc ctcagacaat tgtgtcccga gtgtgtcgtg tgtcctagga
     2701 gcttcatgac tgtcatatat cattcaggca cggatcaaac acacgttatc aaggacttca
     2761 tgaacatcag acctgaaggg gtgagagcac ctatttgtca cccaacgacg cgtatcagaa
     2821 catgtctgct ggtgatgctg tgcgctggat tgggggcttg cgcgcttgtc cccggtgaca
     2881 aagcctacgg cctgcgcgat caccagtcgg ccgtcaaatt gccggtgcgc caggagcccg
     2941 ccgccgagcc ggtgcccgac aacgtcacca tcaagccgat cactgccgag ctgatcatcg
     3001 agcagatgaa gcagtccgcc gccgagcggc cggcggccaa cgcctcccag atgaacggac
     3061 gttccggtgt cggccccatg cccgcctcgc ccgactataa agtgggtccg ggcgacgtgc
     3121 tgaccgttgt cgtgtgggat caccccgaac tcaccacccc ggccggcca
//
LOCUS       DAOQMM010000177         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_21639_length_3159_cov_52.204253, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000177 DAOQMM010000000
VERSION     DAOQMM010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21639_length_3159_cov_52.204253"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PLH85_16800"
     CDS             <1..1883
                     /locus_tag="PLH85_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB66277.1"
                     /translation="EGASLQIHPGWKVGLTGANGCGKSSLFGLLRGELHPDAGDLELP
                     AAWVIAHVAQETPALPRPAIEYVLDGDAELRRIETALTAAEAAHQGEEIGHLHGRLQE
                     IGGYAARARAAALMDGLGFGSADLERPVADFSGGWRMRLNLAQALMCRSDLLLLDEPT
                     NHLDLDAVIWLEQWLTDYRGTLILISHDRDFLDAVVNQIAHIEGQRLTLYAGGYSDFE
                     RQRAERLSQQQALFDKQQRERAHLQKYVDRFRAKATKARQAQSRIKALERMELISAAH
                     VDTPFSFSFRACGAAPDPLLQIEDAAAGYGAAPLLADMSLTIRPGERIGLLGRNGAGK
                     STLIKLLSGELAPLTGVRREGKGLALGYFAQHALERLRPDESPLQHMLRADPQTREQE
                     LRDYLGGFDFRGDGTSSAGAGATTPCGPFSGGEKSRLALALLIWGRPNLLLLDEPTNH
                     LDLEMRHALTLALQDYEGGMVIVSHDRALLRATCDRFLLVDGGRLTPFDGDLDDYKAW
                     LAQRRSADAAAAQCPDRAADKASRKAEREAAAADRQARLAARRPLLKELERLEKQLTP
                     WTAEKASLDERLADPALYTGPDAGEVPKLIKRQAELADRLDAAELRWLELHEALEAIP
                     AD"
     gene            2059..2667
                     /gene="thrH"
                     /locus_tag="PLH85_16805"
     CDS             2059..2667
                     /gene="thrH"
                     /locus_tag="PLH85_16805"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNB66278.1"
                     /translation="MQLVCLDLEGVLVPEIWIEFAERTGIPELRRTTRDEPDYDKLMK
                     YRLDLLKQHKLGLPDIQKVISEMGPEPGAKDFLDALRQDFQVIILSDTFYEFAMPLMQ
                     QLGMPTLFCHKLEANAEGYLVNYHLRMANQKKEAVQRFKEINFKVIAAGDSYNDTAML
                     KEAHAGILFRAPDNVIAEFPQFPVEREYSGLMAQIRAASARI"
     gene            2717..>3159
                     /locus_tag="PLH85_16810"
     CDS             2717..>3159
                     /locus_tag="PLH85_16810"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438003.1"
                     /note="produces formate from formyl-tetrahydrofolate which
                     is the major source of formate for PurT in de novo purine
                     nucleotide biosynthesis; has a role in one-carbon
                     metabolism; forms a homohexamer; activated by methionine
                     and inhibited by glycine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNB66279.1"
                     /translation="MHRERFYTLSASCPDRTGIVAKVSGFIAQHKGWILETALHAEPA
                     TDAEHRGRYFMRIEIRADSLPFMLTEFRERFAPIAEELQMDWKITDSAVKKRVVIMVS
                     KQEHCLYDLLARWQSKELDIEIPCVISNHDTFRGFVEWHGIPFHHV"
BASE COUNT          520 a         1197 c          985 g          457 t
ORIGIN      
        1 tcgaaggcgc cagcctgcag atccaccccg gctggaaggt cggcctcacc ggcgccaacg
       61 gctgcggcaa atcgagcctg ttcggcctgc tgcgcggcga gctgcacccg gacgccggcg
      121 acctggagct gcccgccgcc tgggtgattg cccacgtggc ccaggagacg cccgccctgc
      181 cgcgcccggc catcgagtac gtgctcgacg gcgacgccga gctgcgccgc atcgaaaccg
      241 ccctgaccgc cgccgaggct gcccaccagg gtgaggagat cggccatctc cacggccgcc
      301 tgcaggagat cggcggttac gccgcccgcg cccgcgctgc cgcattgatg gacggtctgg
      361 gcttcggcag cgccgacctg gagcggccgg tggcggattt ctccggcggc tggcgcatgc
      421 gcctcaacct cgcccaggcc ctgatgtgcc gctccgacct gctgctgctg gacgagccca
      481 ccaaccacct ggacctggac gcggtgatct ggctggagca gtggctgacg gactaccgcg
      541 gcacgctgat cctcatctcc cacgaccggg acttcctcga tgcggtggtg aatcagatcg
      601 cccacatcga gggccagcgc ctcaccctct acgccggcgg ctactcggat ttcgagcgcc
      661 agcgcgccga gcgcctgtcc cagcagcagg ccctcttcga caagcagcag cgcgagcgcg
      721 cccacctgca gaagtacgtg gaccgcttcc gcgccaaggc caccaaggcg cgccaggccc
      781 agagccgcat caaggccctg gagcgcatgg agctgatctc cgcggcccac gtggatacgc
      841 ccttcagctt cagcttccgg gcctgtggcg cggcacccga tccgctgctg cagatcgagg
      901 acgccgccgc cggctatggc gcggcgccgc tgctcgcgga catgtcgctg acgatccggc
      961 cgggcgagcg cattggcctg ctgggacgca acggcgccgg caaatccacc ctcatcaagc
     1021 tgctgtcggg cgagctggcg cccctcaccg gggtgcgccg cgaaggcaag ggcctggccc
     1081 tgggctactt cgcccagcac gccctggagc gcctgcgccc ggacgagtcg ccgctccagc
     1141 acatgctgcg cgccgacccc cagacccgcg agcaggagct gcgcgactac ctgggcggct
     1201 tcgacttccg cggcgatggc acgagcagcg ccggggccgg ggcgaccacg ccctgcgggc
     1261 ccttctccgg cggcgagaaa tcgcgcctcg ccctggccct gctgatctgg ggccgaccca
     1321 acctgctgct gctggacgaa cccaccaacc acctggacct ggagatgcgc cacgccctga
     1381 ccctggcgct ccaggactac gagggcggca tggtaatcgt gtcccacgac cgggccctgc
     1441 tgcgcgccac ctgcgaccgc ttcctgctgg tggatggcgg tcgcctgacg cccttcgacg
     1501 gtgacctgga cgactacaag gcctggctcg cccagcgccg cagcgccgac gcggccgccg
     1561 cccaatgtcc cgaccgggcc gccgacaagg ccagccgcaa ggccgagcgg gaagccgccg
     1621 ctgccgaccg tcaggcccgc ctcgccgccc gccggccgct gctgaaagaa ctcgaacgcc
     1681 tggaaaagca gctcacgccg tggaccgccg aaaaggcgag cctcgacgaa cgccttgccg
     1741 acccggcgct gtacaccggc ccggacgcgg gcgaggtgcc taagctgatc aagcgccagg
     1801 ccgagctggc cgacaggctc gacgccgccg agctgcgctg gctggagctc cacgaggcgc
     1861 tggaagcgat tccggcggat tgacgcgccc ggcgcccgcc tcgttcccgt ctccaccgcc
     1921 ctggcccgcg cgggcgcgtc cggggtgtgc aggtggccgg cgccgcgcgc cgcctgccac
     1981 agacttcctc aaggcgtctc cgtgtctttg ctagaatcgc ggcttcgaaa tcaaccgtcg
     2041 tactctggag tccacgccgt gcaactcgtc tgtctcgacc tcgaaggtgt cctcgtcccc
     2101 gaaatctgga tcgaattcgc ggagcgcacc ggcattcccg aactgcgtcg caccacccgc
     2161 gacgagccgg actacgacaa actcatgaag taccgcctgg atctgctcaa gcagcacaag
     2221 ctgggtctgc cggacatcca gaaggtcatc tccgagatgg gccccgagcc cggtgccaag
     2281 gacttcctcg acgccctgcg ccaggatttc caggtgatca tcctgtccga caccttctac
     2341 gaatttgcca tgccgctgat gcagcagctg ggcatgccga ccctgttctg ccacaagctg
     2401 gaggcgaacg ccgagggtta cctggtgaac taccacctgc gcatggccaa ccagaagaag
     2461 gaagcggtgc agcgcttcaa ggaaatcaac ttcaaggtca tcgccgccgg cgattcctac
     2521 aacgacaccg cgatgctcaa ggaagcccac gccggcatcc tcttccgcgc cccggacaac
     2581 gtcatcgccg agtttcccca gttccccgtg gagcgcgagt acagcggcct gatggcccag
     2641 atccgcgccg cctccgcccg catctgaccc ttctgcagcg ccgcctcgcg cccggcgcgg
     2701 ggggcgcgag accatcatgc accgcgaacg cttctacacc ctgtccgcct cctgccccga
     2761 ccgcaccggc atcgtcgcca aggtttcggg cttcatcgcc cagcacaagg gctggatcct
     2821 cgagaccgcc ctccacgccg aaccggcgac ggacgccgag caccgcggac gctacttcat
     2881 gcgcatcgag atccgcgcgg attcgctgcc cttcatgctc accgagttcc gcgagcgctt
     2941 cgcgcccatc gccgaagaat tgcagatgga ctggaagatc accgattcgg cggtcaagaa
     3001 gcgcgtggtg atcatggtct ccaagcagga gcactgcctc tacgacctgc tcgcccgctg
     3061 gcagtcgaag gagctggaca tcgagatccc ctgcgtgatc tccaaccacg acaccttccg
     3121 cggcttcgtg gaatggcacg gcatcccctt ccaccacgt
//
LOCUS       DAOQMM010000178         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_23784_length_2990_cov_37.294037, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000178 DAOQMM010000000
VERSION     DAOQMM010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23784_length_2990_cov_37.294037"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLH85_16815"
     CDS             complement(<1..793)
                     /locus_tag="PLH85_16815"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB66280.1"
                     /translation="MPLSLLRLPSLDLVRGFVAVGRRMSITVAAADLCLTQSAVSRQI
                     HALEDHLGLKLFVRGHRSIAFTPEGERLFRSADGAFQQLQDVLGDLRAAGALRPVTVS
                     ACIGIAGLWLLPRLSRFQALHPGVDLRVSAVNRIADLRHEGIDLAIRYAPDDAVPDGA
                     ERLFGETVVPVAHAGLAGVDLADDGALAGVNLLEFDDPGRPWLQWSTWLSAQGRAAPR
                     PKGVLHFNQYDQVIQAALAGQGVALGRLALIRPLLDEGRLVCVAGP"
     gene            929..1762
                     /locus_tag="PLH85_16820"
     CDS             929..1762
                     /locus_tag="PLH85_16820"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNB66281.1"
                     /translation="MTPPNRPSQHALADTFRALHADPAGFLIPNPWDAGTARLLAHLG
                     FRALASTSAGFSFGRGHADRSVPRGPLLAHLVELTAATALPVSADLENGFGDAPSTVA
                     ETIRLAAAAGVVGGSIEDATGRADAPLYDIPFAAERIRAAVEAARALPFPFTLTARAE
                     NFFIGRGDLADTIRRLQAYQEAGADVLFAPGISRTEDIATLLREVDRPVNVLLGAPGM
                     SVDAPTLFAMGVRRVSVGGSLARAALGAFLRAATELRDRGTTDYTRDAVSGKELNRLF
                     G"
     assembly_gap    1768..1867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1881..2795)
                     /locus_tag="PLH85_16825"
     CDS             complement(1881..2795)
                     /locus_tag="PLH85_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase"
                     /protein_id="HNB66282.1"
                     /translation="MSDSLYRNPLARIVTSPGEILVRVAAAAAGPTGEVDAEMRALMA
                     RQVAAGELLDMNPAWIWPDFAAGLMRDAPSKMLWVLRETGALRYLLPELDALFGVPQS
                     ADDPPTVDIGEHQLRVVDAVARQHAPLAVRFAALLYNLGKADSPPEHLPSHYRHMERG
                     APRVEAVCARFGVTAEVRELALLALREVERVHRAAEMRAGSIAALLERVDAFGQPERF
                     ARLMTLCACDFHAFPGRATRPYPKARLLATALEACRGIDPAALQEDHADDPEAAAQAL
                     LEARAMAVARALRSERWDDSAQEEAAAD"
     gene            2794..>2990
                     /locus_tag="PLH85_16830"
     CDS             2794..>2990
                     /locus_tag="PLH85_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66283.1"
                     /translation="MAKAVMGGRTTKATRVWGSRRPQKHPRDSRKGPQHVGCRATGGR
                     DDDDGREGYSKMNAGWPGVTAG"
BASE COUNT          400 a         1077 c         1075 g          338 t
ORIGIN      
        1 cggggccggc cacgcagacg aggcgacctt cgtccagcaa ggggcggatc agcgccagcc
       61 ggccgagggc cacgccctgg ccggcgaggg ccgcctggat cacctggtcg tactggttga
      121 agtgcagcac gcccttgggc cgtggggcgg cgcggccctg ggcggagagc caggtgctcc
      181 actgcagcca ggggcggccg gggtcgtcga attccagcag gttgacgccc gccagggccc
      241 cgtcgtccgc cagatccacc cccgccaggc cggcgtgggc caccggcacc acggtctcgc
      301 cgaacaggcg ctcggcgccg tccggcacgg cgtcgtcggg ggcgtagcgg atggccaggt
      361 cgatgccctc gtggcgcagg tcggcgatgc ggttgaccgc cgagacgcgc aggtccacgc
      421 cggggtgcag ggcctggaag cggctcaggc gcggcagcag ccacaggccg gcgatgccga
      481 tgcaggcgct caccgtgacc gggcgcaggg ccccggcggc gcgcaggtcg cccagcacgt
      541 cctgcagctg ctgaaaggcg ccgtcggcgc tgcggaacag gcgctcgccc tcgggggtga
      601 aggcgatgga acgatgcccg cgcacgaaga gcttgaggcc caggtggtcc tccagggcgt
      661 ggatctggcg gctcaccgcc gactgggtga ggcacaggtc ggcggcggcg acggtgatgc
      721 tcatgcgccg cccgacggcg acgaagccgc gcaccaggtc gagggagggc agacggagga
      781 ggctcagggg cattcccatg gcgcatgcga agggacggaa aagtcgattg aaccacaggc
      841 cgcgggcgcg tgtaatgggc gggacgctgc cgcgcccggc gcgcagcccg tcgcccgccc
      901 cgcttcttca ttccgacaag gatctgcgat gacgcctccc aaccgcccgt cccagcacgc
      961 gctggccgac accttccgcg ccctccacgc cgaccccgcc ggcttcctca tccccaaccc
     1021 gtgggacgcg ggcaccgcgc gcctgctggc gcatctgggc tttcgcgcgc tggccagcac
     1081 cagcgccggg ttctccttcg gccgcggcca tgcggaccgc tcggtgcccc gcggccccct
     1141 gctggcgcac ctggtggagc tcaccgcggc caccgccctg cccgtcagcg ccgatctgga
     1201 gaacggcttc ggcgacgccc cgtccacggt ggccgagacc atccgcctgg ccgccgcggc
     1261 gggcgtggtg ggcggctcca tcgaagacgc cacgggccgc gcggacgccc cgctgtacga
     1321 catccccttc gccgcggagc gcatccgcgc cgccgtggag gcggcccgcg ccctgccctt
     1381 ccccttcacc ctcaccgccc gcgcggagaa cttcttcatc ggtcgcggcg acctcgccga
     1441 caccatccgc cgcctgcagg cctaccagga ggccggcgcc gacgtgctgt tcgcccccgg
     1501 catcagccgc acggaggaca tcgccaccct gctgcgggag gtggaccggc cggtgaacgt
     1561 gctgctcggc gcgccgggca tgagcgtgga cgcgccgacc ctcttcgcca tgggggtgcg
     1621 ccgggtcagc gtgggcggtt ccctggcgcg ggcggcgctg ggggccttcc tgcgcgccgc
     1681 caccgagctg cgcgaccggg gcaccacgga ctacacccgc gacgcggtga gcggcaagga
     1741 gctgaaccgg ctgttcggct gaggcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnngcg gcgcgggcgc ctaatccgcc gcggcttctt cctgggcgct gtcgtcccag
     1921 cgttccgagc gcagggcgcg ggccacggcc atcgcgcggg cttccagcag ggcctgggcg
     1981 gcagcctccg gatcgtcggc gtggtcctcc tgcagcgccg cggggtcgat gccgcggcag
     2041 gcctccaggg ccgtggcgag caggcgcgcc ttgggataag gccgcgtggc gcggccgggg
     2101 aaggcgtgga agtcgcaggc gcacagggtc atcaggcggg cgaagcgctc cggctggccg
     2161 aaggcgtcca cccgctccag cagcgcggcg atggagccgg cgcgcatctc ggcggcccgg
     2221 tgcacccgct ccacctcccg cagggccagc agggccagct cgcggacctc ggcggtcacg
     2281 ccgaagcgcg cgcacaccgc ctccacccgc ggcgcgcccc gctccatgtg gcggtagtgg
     2341 gacggcaggt gctcgggcgg cgaatcggcc ttgcccaggt tgtagagcag cgccgcaaag
     2401 cgcaccgcca gcggcgcatg ctggcgcgcc acggcgtcca ccacgcgcag ctggtgctcg
     2461 ccgatgtcca cggtgggcgg atcgtcggcg ctctggggca cgccgaagag ggcgtccagc
     2521 tccggcagca ggtagcgcag cgcgccggtc tcccgcaaga cccacagcat cttcgacggc
     2581 gcgtcgcgca tcagccccgc cgcaaaatcc ggccagatcc acgccggatt catgtccagc
     2641 agctcccccg ccgccacctg ccgggccatc agggcgcgca tctccgcgtc cacctccccc
     2701 gtcggccccg ccgcagccgc cgccacgcgc acgaggatct cccccggcga ggtgacgatg
     2761 cgggcgaggg gattgcggta gagggaatcg gacatggcga aggctgtgat gggtggaagg
     2821 acgacaaaag cgacccgcgt gtggggttcg cgacgcccgc aaaaacaccc ccgcgacagc
     2881 cgcaaaggcc cgcagcacgt gggatgtcgg gccacgggcg ggcgcgacga cgacgacggt
     2941 cgggagggtt acagcaagat gaatgccggg tggccgggcg tcaccgcggg
//
LOCUS       DAOQMM010000179         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_24596_length_2932_cov_47.635384, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000179 DAOQMM010000000
VERSION     DAOQMM010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24596_length_2932_cov_47.635384"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLH85_16835"
     CDS             <1..835
                     /locus_tag="PLH85_16835"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HNB66284.1"
                     /translation="ASSPAAATDPQARLAAAAQAAQAALQKGPRDIALAGQGVLKLPE
                     GFGFIPAGPAGEFLKAQGSRVGPDFQGLVVGDAIDGFVVVRFEDAGYVKDEEAREWDA
                     AKLLDNLKSGTEAANEDRRKLGMPEFSVQGWVEAPRYDEANHRLVWSASTREKNGAAS
                     DGDGVNYNTYVLGREGYFSMNLVTSLATVEQQKPVAQQLLAALSFDEGKRYADFNSST
                     DKVAEYGLAALVGGIAAKKLGLLATLGVFLAKFWKVAALAVVGAGAGLRKLFGRKDGQ
                     A"
     gene            832..1548
                     /locus_tag="PLH85_16840"
     CDS             832..1548
                     /locus_tag="PLH85_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB66285.1"
                     /translation="MKLLLLLLSGLKFGKLLTTGGTMLLSVAVYAFVWGWRYAVGFVV
                     LLFCHEMGHYIAARQRGLHVGVPTFIPFVGAWIDLKEQPMNAETEAYVAMAGPLAGTL
                     ASVACYFLARSEGSDLLLAISYSGFFLNLFNLIPVPPLDGGRMTGVVSRRFWLLGAPV
                     LVGLFLWRPSPMLILVAILAAPHMLRAFRGEGGEAPGYYDTPAGKRFEYALLYLGLLA
                     YLAVMSYEVHEMLGAARGGL"
     gene            1831..2928
                     /locus_tag="PLH85_16845"
     CDS             1831..2928
                     /locus_tag="PLH85_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HNB66286.1"
                     /translation="MSFRALPARALAAAGVVLATLVSPALQAAPPLPALNIDVNQTSV
                     SGISSGGFMSVQFQVAHSSIVAGAGVVAGGPYACAQGDTTRAVSQCSCTGKPFVACQV
                     TDHSADVPTLTDKVKTLAAQGAIDAPSHLARQRIVTVSGAKDTLVPPAIAQQLDAFYA
                     ALGVPAAQISPVSLPNAAHTMPTSSYGIACGKEDAPYIGKCGYDSAGAILQWIYGALT
                     PAGAKAAGSFSEFDQRRYVPEERRDYTGFKSGMDATGWLYVPNSCRKGERCRVHIVLH
                     GCKQGQSYMPLRPPPDGGLYNGTLFVKHTGYDRWADNNHLVILYPQALTVPGNPNGCW
                     DWWGYTGQDYATREGVQIRTLRAMVDALAGQ"
BASE COUNT          409 a          973 c         1095 g          455 t
ORIGIN      
        1 ggcctcgtcg cccgcggcgg ccaccgatcc gcaggcgcgg ctggcggcgg ctgcgcaggc
       61 ggcccaggcg gcgctgcaga aggggccgcg ggacattgcg ctggccgggc agggcgtgct
      121 caagctgccg gagggcttcg gcttcatccc cgccgggccg gcgggggaat tcctgaaggc
      181 gcagggctcc cgcgtggggc cggactttca gggtctggtg gtgggcgatg cgatcgacgg
      241 ctttgtggtg gtgcgcttcg aggacgcggg ctacgtgaag gacgaggagg cccgcgagtg
      301 ggacgcggcg aagctgctgg acaacctgaa gtccggcacc gaggcagcca acgaggaccg
      361 ccgcaagctg ggcatgccgg agttcagcgt gcagggctgg gtggaggcgc cccgctacga
      421 cgaggcgaac caccgcctgg tgtggtcggc ctctacccgc gagaagaacg gggccgcgag
      481 cgacggggac ggggtgaact acaacactta tgtgctgggc cgggagggtt acttctccat
      541 gaacctggtg accagcctgg cgaccgtgga gcagcagaag ccggtggccc agcagctgct
      601 ggcggccttg tcctttgacg aaggcaagcg ctacgcggac ttcaactcct ccaccgacaa
      661 ggtggcggaa tacggtctgg cggcgctggt ggggggcatc gcggcgaaga agctgggcct
      721 gctggcgacg ctgggggtgt tcctggccaa gttctggaag gtggcggcgc tggcggtggt
      781 gggcgccggc gccgggctgc gcaagctctt cgggcgcaag gacgggcagg cgtgaagctg
      841 ctcctgctgc tgctctcggg gctgaagttc ggcaagctgc tcaccaccgg cggcaccatg
      901 ctgctgtcgg tcgcggtgta tgccttcgtg tggggctggc gctacgcggt gggcttcgtg
      961 gtgctgctgt tctgccatga gatgggccat tacatcgcgg cgcgccagcg cgggctgcac
     1021 gtgggcgtgc cgaccttcat ccccttcgtg ggggcgtgga tcgacctgaa ggagcagccc
     1081 atgaacgcgg agaccgaggc ctatgtggcg atggccgggc cgctggcggg cacgctggct
     1141 tcggtggcgt gctacttcct ggcccgctcg gaggggagtg acctgctgct ggcgatcagc
     1201 tattcgggct tcttcctgaa tctcttcaac ctgatcccgg tgccgcccct ggacggcggg
     1261 cgcatgaccg gcgtggtgtc gcggcgcttc tggctgctgg gagcgccggt gctggtgggc
     1321 ctgttcctgt ggcggccgag cccgatgctg atcctcgtgg cgatcctcgc cgcgccccac
     1381 atgctccggg ccttccgcgg cgaagggggc gaggcgccgg gctattacga cacgccggca
     1441 ggcaagcgct tcgagtacgc gctgctttac ctgggcctgc tggcctatct ggcggtgatg
     1501 agctacgagg tgcacgagat gctgggcgcg gcgcgcggcg gcctgtgagg acgacggcgc
     1561 atcggcatgg ggcggcacgc ggggcgcggc cctgagacgc ggcccggagc gggacagcac
     1621 ggggtgcgcg ggaaggaccg cgcgggggcc gcgaccttcc cacgggcggg ccgttccctc
     1681 ccccacaggt ctgaaatcga cctgccgggc gacgtcaccg aaaattcttt tcgcatgagg
     1741 cttcttgggc taaggtgatg ttgcggcgca tcacgcgcgt gcttcgcccg gcgcccttgc
     1801 cggccctccc gacaaaccct ggagttgccc atgtccttcc gtgccctgcc ggcccgcgcc
     1861 ctggccgccg ccggtgtcgt cctcgcgacg ctggtgtcgc ccgccctgca ggcagcgccg
     1921 ccgctgcccg ccctcaacat cgacgtgaat cagacctcgg tctccggcat ctcgtccggg
     1981 ggcttcatga gcgtgcagtt ccaggtggcc cattcgtcca tcgtggcggg cgccggggtg
     2041 gtggcgggcg ggccctacgc ctgcgcccag ggcgacacga cgcgggcggt gtcccagtgc
     2101 tcgtgcaccg gcaagccgtt tgtcgcctgc caggtgacgg accacagcgc cgacgtgccg
     2161 accctcaccg acaaggtgaa gaccctggcc gcccagggcg ccatcgacgc gccgtcccat
     2221 ctggcgcggc agcgcatcgt gacggtgtcc ggtgccaagg acaccctcgt gccgccggcc
     2281 atcgcgcagc agctggacgc cttctatgcc gccctcggcg tgccggcggc gcagatctcg
     2341 ccggtgtccc tgcccaacgc ggcccacacc atgccgacca gcagctacgg catcgcctgc
     2401 ggcaaggagg acgcgcccta catcggcaag tgcggctacg acagcgccgg ggcgatcctg
     2461 caatggatct acggcgcgct gacgccggcc ggggccaagg cggcgggcag cttctcggag
     2521 ttcgaccagc gccgctacgt gccggaggaa cgccgcgatt acacgggctt caagtcgggc
     2581 atggacgcga ccggctggct ctacgtgccc aacagctgcc gcaagggtga gcgctgccgc
     2641 gtgcacatcg tgctgcacgg ctgcaagcag ggccagagct acatgcccct gcgcccgccg
     2701 cccgacggcg gcctgtacaa cggcaccctg ttcgtgaagc acacgggcta cgaccgctgg
     2761 gccgacaaca atcacctggt gatcctctat ccccaggccc tcacggtgcc cggcaacccc
     2821 aacggctgct gggactggtg gggctacacg ggacaggatt acgcgacccg cgagggcgtg
     2881 cagatccgca cgctgcgggc gatggtggat gcgctggcgg ggcagtgagg gg
//
LOCUS       DAOQMM010000180         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_24613_length_2931_cov_58.859527, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000180 DAOQMM010000000
VERSION     DAOQMM010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24613_length_2931_cov_58.859527"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLH85_16850"
     CDS             complement(<1..449)
                     /locus_tag="PLH85_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB66287.1"
                     /translation="MSAVWIVDDDRSIRWVLEKALAREEIPYKSFGNANDVLTALDSA
                     AQPPRVLVSDIRMPGESGLTLLSKVKARFPHLPVIIMTAYSDLDSAVAAFQGGAFEYL
                     PKPFDVDQAVELVRRAIDETAHQHAGEEVLSAVPEILGQAPSMQEVFR"
     gene            complement(526..1599)
                     /gene="glnL"
                     /locus_tag="PLH85_16855"
     CDS             complement(526..1599)
                     /gene="glnL"
                     /locus_tag="PLH85_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNB66288.1"
                     /translation="MQNDPPDQTPSPFAGLELLSSAVVLVDDELIVRYLNPGAENLFA
                     VSSRKIVGYSLRRLLGEPAGLGTALNNVLRDNWSYTGHNLTVLRPGMEALHLDCTVTP
                     VESDAVRLLLEFRPMDAHLKVAREEQLAHQQQANRELIRNLAHEIKNPLGGIRGSAQL
                     LERELDDPQLREYTQVIIAEADRLQDLMNRLLTSHRMMQPSQLNVHDVLERVRRLVLA
                     EFPSVEVRRDYDTSLPYVTGDREQLIQAILNITRNAAQAMNGDGEIVLRTRAARQITM
                     AKRRFKLALQLQVIDNGPGIPAEIRDKIFYPLVSGRENGSGLGLSLAQSFIEQHQGVI
                     DVDSRPGRTCFTIFLPITRDSDV"
     gene            complement(1677..2177)
                     /locus_tag="PLH85_16860"
     CDS             complement(1677..2177)
                     /locus_tag="PLH85_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNB66289.1"
                     /translation="MKLPVVLGLAALLSAAPALADIFKCIDEGGHVTYTNAKVGGKGC
                     SLLSRDQPVSSMPAGPASRPAGAPSTAPAAPSASGAAGFPRVDAGTQRARDNDRRRIL
                     EDELATEQNSLAVAQKDLADQEAIRNGDERNYQRVLDRLQPFKDKVQLHERNIEALRR
                     EIANLR"
     gene            complement(2344..>2931)
                     /gene="glnA"
                     /locus_tag="PLH85_16865"
     CDS             complement(2344..>2931)
                     /gene="glnA"
                     /locus_tag="PLH85_16865"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434326.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNB66290.1"
                     /translation="IWKDGKNLFAGNGYAGLSETALYYIGGIIKHAKALNAITNPLTN
                     SYKRLVPHFEAPVKLAYSAKNRSASIRIPFVNSEKARRIETRFPDPGANPYLCFAALM
                     MAGLDGIQNKIHPGDPADKNLYDLPPEEDALIPTVCSSLEQALEALDKDREFLTRGGV
                     FSNDFIDSFIALKNDEVNRLRVEVHPVEFEMYYAI"
BASE COUNT          507 a          901 c          981 g          542 t
ORIGIN      
        1 cggaagactt cctgcatgga cggcgcctgg ccgaggatct cgggcaccgc gctgagaact
       61 tcctcaccag cgtgctggtg ggcggtctcg tcgatggcac gacgcaccag ctcgaccgcc
      121 tggtccacat cgaagggctt gggcaggtat tcgaaagcgc ccccctggaa ggccgccaca
      181 gcactgtcca gatcggagta ggcagtcatg atgatgaccg gcaggtgcgg gaagcgtgcc
      241 ttgaccttgg acagcagggt cagccccgac tcgcccggca tgcggatgtc ggacaccagc
      301 acgcgcggcg gctgggcggc actgtcgagc gccgtcagca cgtcgttggc attaccgaaa
      361 ctcttgtagg ggatttcctc ccgcgccagg gctttctcca gcacccaacg gatggagcgg
      421 tcatcatcga ctatccagac tgcactcatt ttctctcaca cgctcaggga ggccaggcct
      481 cgacgtcaac tgttggcacc acattggtgc attctcttca ggggatcaca catcgctgtc
      541 ccgcgtgatg ggcaggaaga tcgtgaaaca ggtccggcca ggccgggagt ccacgtcgat
      601 caccccctga tgctgctcga tgaagctctg agccagggac aatcccagtc cgcttccgtt
      661 ctcccgcccc gacaccagcg ggtagaagat cttgtcgcgg atctcggcgg gaatgcccgg
      721 gccgttgtcg atcacttgca actgtaatgc cagtttgaag cggcgcttgg ccatggtgat
      781 ctgacgggcc gcgcgggtac gcaggacaat ctcgccgtcg ccattcatgg cctgcgcggc
      841 attgcgggtg atgttgagga tggcctggat gagctgctcg cggtcaccgg tcacgtaggg
      901 caggctggta tcgtagtcgc ggcgcacctc gacggaggga aactccgcca gcaccagacg
      961 gcgcacgcgt tccagcacgt catgcacgtt gagttgcgac ggctgcatca tgcggtggga
     1021 cgtgagcagg cggttcatca ggtcctgcag gcggtccgct tcggcaatga tgacctgggt
     1081 atattcgcgg agctgcgggt cgtccagttc ccgctccagc agctgggccg acccccggat
     1141 gccgcccagc ggattcttga tctcgtgcgc cagattgcgg atcagctcgc ggtttgcctg
     1201 ttgttggtgc gcgagctgct cttcccgtgc cacttttagg tgcgcatcca tggggcggaa
     1261 ctccagcagg aggcgcaccg catcggactc gacgggcgtg acggtgcaat ccaggtgcaa
     1321 ggcttccatt ccggggcgca ggaccgtgag attgtggccg gtgtagctcc agttgtcccg
     1381 cagcacgttg ttcagggccg tgccgagccc ggccggctca ccgagcaggc gacgcagaga
     1441 gtagccgacg atcttgcggc tgctcacggc aaacaggttc tcggcccccg gattgagata
     1501 gcggacgatc agctcgtcgt ccacaagcac cacggccgac gacagcagct cgaggccggc
     1561 aaagggcgaa ggggtctggt caggcgggtc gttttgcata gcttgcaagc atgcaagaaa
     1621 cagaccaaca ggcaccgcct ctgggccctt gcgggcaccg cccaaccggc tgaagatcag
     1681 cgcagattgg cgatctcgcg gcgcagggct tcgatgttgc gctcgtggag ctggaccttg
     1741 tccttgaacg gctgcaggcg gtcgagcacg cgctgatagt tgcgttcatc cccgttgcgg
     1801 atcgcctcct ggtcggccag atccttctgg gccaccgcca ggctgttctg ttcggtggcg
     1861 agctcgtctt cgagaatgcg ccgacgatca ttgtcccgcg cccgctgggt gcccgcatcg
     1921 acacgcggga agccggccgc cccggatgcg gacggcgctg caggcgccgt cgaaggcgca
     1981 ccagcaggcc gcgacgccgg cccggccggc atcgacgaga ccggctgatc ccgcgacagc
     2041 agactgcacc ctttgccgcc caccttggca ttggtgtacg tgacatgccc gccctcgtcg
     2101 atgcatttga aaatgtcggc gagggccggg gcggcggaca gcagggcggc gaggcccagg
     2161 acgacgggaa gcttcatggt ctcgaacggg cgcgcaggcg cgttgaacac ggccccccta
     2221 gtgtatgtgc cccctgttgc aaacacaaac gcggtcctgt aacaaaaaaa ggacggaatt
     2281 gcttccgtcc ttttcaggca ccacgcgccg gccggaaacc cggccggcac tgatcgcgca
     2341 ggatcagatg gcgtagtaca tctcgaactc gaccgggtgc acttcgacgc gcagacggtt
     2401 cacttcgtcg ttcttgaggg cgatgaagga gtcgatgaag tcattgctga acacgccacc
     2461 gcgggtcagg aactcgcggt ccttgtccag ggcttccaga gcctgttcga ggctggagca
     2521 gacggtcggg atcagcgcgt cttcttccgg cggcagatcg tacaggttct tgtcggcggg
     2581 gtcgcccggg tggatcttgt tctggatgcc gtccaggccg gccatcatca gcgcagcgaa
     2641 gcacaggtag gggttcgcgc ccggatccgg gaaacgggtc tcgatgcggc gggccttttc
     2701 gctgttgacg aacgggatgc ggatcgaagc ggaacggttc ttggcggagt aggccagctt
     2761 caccggggct tcgaagtggg gcaccagacg cttgtaggag ttggtcagcg ggttggtgat
     2821 cgcgttcagg gccttggcgt gcttgatgat gccgccgatg tagtacaggg cggtctcgga
     2881 caggccggcg tagccgttgc ccgcgaacag gttcttgccg tccttccaga t
//
LOCUS       DAOQMM010000181         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_25193_length_2892_cov_55.524145, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000181 DAOQMM010000000
VERSION     DAOQMM010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25193_length_2892_cov_55.524145"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLH85_16870"
     CDS             <1..590
                     /locus_tag="PLH85_16870"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HNB66291.1"
                     /translation="ESLEDQARTLVRAVTMFRLAAGAPAAPASLDFDGAIQAHMQWKH
                     KLRNFLAGEGDPLDADVVSRDDKCVLGCWIHGEGQRFGHMSSYTRLKHKHAAFHACAG
                     SVIRAKQAGDEAAANALLMGDFAVLSDETIREIRDLRAVCGSQAATPAAAAPAARAPS
                     NVSPLPVRNLKSGAPLPKVKQSAPSNLAENEWEEF"
     gene            641..>2892
                     /locus_tag="PLH85_16875"
     CDS             641..>2892
                     /locus_tag="PLH85_16875"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNB66292.1"
                     /translation="MKINMPVTQREIELGDAEVIVSKTDLKGQITYVNREFTAISGFS
                     EDELIGKSHNIVRHPDMPPEAFEDMWRDLKAGRPWVGIVKNRCKNGDHYWVEAHATPV
                     RENGAITGYMSVRRKPTRAQIEAAGHAYALFRDGKAGGRAICHGQVVSAGLQRRLVHG
                     FANLSVAAKAAVATALCLSLILGGGFLLLRERTTAMLESQAMSVVTDKVAAVKALVEV
                     AAASAKRETDRLNDVFVAGFPEGFVAHEGKDGVPVLSSGAVVLNQRTLEVDRFSGASG
                     AAVTVFVRKGDEFIRVASSLKQENGERLTGSTLDHDNPAYAKLVSGQRYTGRAQLSGR
                     DIYASYAPLKDAAGKVIGGVFVGRRFAEELEALKAQVKAVRFGETGYAYVLDGTPGKS
                     QGTLMVHPNKEGQNLLDAKDTTGRSIVREMLERKQGAMTYPWVNAEQNEQVVRNKVVA
                     FDSFPDWNWVIVGGTNVEEFESLAAAVSRQEAVIGTTATIALCVLVYFLLRRFVAAPL
                     RLSVAAAQTVASGRYDYPMDVTRNDEIGQLLQALESMQVKLGNDIAEQKRIGDENARI
                     RQALDSVETNVRIADDSGRVIYANMALMKTVRAIEAEIRQHAPGFRADTFVGSSIGLL
                     YPDPQAALGRLAALQQTVRTQMVIGGRNFEVVTSPIFNAAGTRLGSVGEWRDQTDELA
                     AEKEIQAIVAAAGDGDFSARLDIDGKTGFFRDLSGGINRLMEGVSASLNDLARVLNAI
                     ARGDLTEKITA"
BASE COUNT          517 a          910 c          998 g          467 t
ORIGIN      
        1 cggagagtct ggaggatcag gcccgcacgc tggtgcgggc ggtcaccatg ttccgcctcg
       61 ctgccggagc ccccgcggcg cctgcgtcgc tggatttcga cggggcgatc caggcccaca
      121 tgcagtggaa gcacaagctg cgcaatttcc tggcaggcga aggcgatccg ctcgatgcgg
      181 acgtggtctc gcgggacgac aaatgcgtgc ttggatgctg gatccacggt gaagggcagc
      241 gttttggtca catgtcgtcc tacacgcggc tcaagcacaa gcatgcggcc ttccatgcct
      301 gcgccggttc ggtgatccgc gccaagcagg ccggtgacga ggccgccgcc aacgccctgc
      361 tgatggggga tttcgctgtg ctgtccgacg agacgatccg tgagatccgc gacctgcgcg
      421 ccgtatgtgg ttcgcaggcc gccacgcccg ctgccgcggc acccgctgcc cgtgcgccca
      481 gcaatgtctc gcccctgccg gtgcgcaacc tcaagtccgg agcgccgctg ccgaaagtca
      541 agcagagcgc accttccaat cttgccgaaa acgagtggga agagttctga cgccttgatg
      601 ccaatccctg tcgtcgcgac ggggccacag ggagtacccg atgaagatca acatgccggt
      661 cacccagcgg gagatcgaac taggcgacgc cgaggtcatc gtctccaaga cggacctgaa
      721 gggccagatt acctacgtca accgtgaatt caccgcgatc agcggctttt ccgaggatga
      781 actgatcggc aagtcccata acatcgtccg tcatccggac atgccgccgg aagccttcga
      841 ggacatgtgg cgcgacctca aggcgggccg gccgtgggta gggattgtca agaaccggtg
      901 caagaacggc gaccattact gggtcgaggc ccatgccacg ccggtccggg agaacggagc
      961 gatcaccggg tacatgtcgg tccggcgcaa gccgacccgc gctcagatcg aggccgccgg
     1021 gcatgcctat gccctgttcc gcgacggcaa ggccggcggg agggcgatct gtcacggcca
     1081 ggtagtgtcc gccgggctgc aacgccgtct ggtgcacggc tttgccaatc tttccgtcgc
     1141 ggccaaggcc gccgtggcga cggccctgtg tctctccctg atcctcggcg gcggcttcct
     1201 gctgctgcgc gagcggacga cggcgatgct ggagtcccag gcgatgtccg tggtgaccga
     1261 caaggtggcc gcggtgaagg cgctggtcga ggtggctgcc gcgtccgcca agcgggagac
     1321 ggaccggctc aacgacgtgt tcgtggcggg ctttcccgag ggctttgtgg cacacgaggg
     1381 caaggacggg gttcctgtgc tcagcagcgg tgcggtggtg ctcaatcagc gcacgctgga
     1441 agtcgatcgc ttcagcgggg cgagcggcgc cgccgtgacg gtgttcgtgc gcaagggcga
     1501 tgagttcatc cgggtggcct cgtccctcaa gcaggagaac ggcgaacgtc tgaccggctc
     1561 caccctcgat cacgacaatc cggcctacgc aaagcttgtg tccggtcagc gctacaccgg
     1621 acgcgcccag ctgtccggac gggacatcta cgcgagctac gcgcccctca aggatgcggc
     1681 cggcaaggtg atcggcggcg tgttcgtcgg gcgtcgcttt gccgaagagc tcgaggccct
     1741 gaaggcccag gtcaaggcgg tccgcttcgg tgaaacgggc tacgcctacg tgctggatgg
     1801 cacccctggc aagtcgcagg ggaccttgat ggtgcatccg aacaaggaag ggcagaacct
     1861 gctggacgcc aaggacacca ccgggcgttc gattgtgcgg gaaatgctgg agcgcaagca
     1921 gggtgcgatg acgtacccct gggtgaacgc cgagcagaac gaacaggtcg tgcggaacaa
     1981 ggtcgtggcc ttcgacagct ttccggactg gaactgggtg atcgtgggcg gcaccaacgt
     2041 cgaggagttt gaaagtctgg ccgcggccgt aagcaggcag gaggccgtca tcggcacgac
     2101 cgccaccatt gcgttgtgcg tgctggtgta cttcctgctg cgccgcttcg tggccgcgcc
     2161 cctgcggctc agcgtggccg ccgcccagac cgtggcgagc gggcgctacg actatcccat
     2221 ggatgtgacc cgcaacgacg agatcggtca gctgttgcag gcgctggagt ccatgcaggt
     2281 caagctcggc aacgacatcg ccgaacagaa gcgcatcggc gacgagaatg cgcgcatccg
     2341 tcaggcgctc gacagtgttg aaacgaatgt acggatcgcc gacgacagcg gccgggtgat
     2401 ctacgccaac atggcgctga tgaagaccgt gcgcgccatc gaggccgaga tccgccagca
     2461 tgcgccgggc ttccgggcgg acaccttcgt cggcagcagc atcggcctgc tctatcccga
     2521 ccctcaggct gccctgggac ggctggcagc gttgcagcag accgtgcgga cccagatggt
     2581 gatcggcggg cgtaatttcg aagtggtgac cagcccgatc ttcaatgcgg ccgggacccg
     2641 cctcgggtcc gtgggcgaat ggcgcgacca gaccgacgaa ctggcggcgg agaaggagat
     2701 ccaggccatc gtggcggcgg cgggcgatgg cgatttctcc gcgcgtctgg acatcgacgg
     2761 caagacaggc ttcttccgtg acctgtcggg cggcatcaac cgcctgatgg aaggcgtctc
     2821 ggccagtctc aacgacctgg cccgcgtgct caacgcgatt gcgcggggcg acctgacgga
     2881 gaagatcacg gc
//
LOCUS       DAOQMM010000182         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_26155_length_2828_cov_60.799495, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000182 DAOQMM010000000
VERSION     DAOQMM010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26155_length_2828_cov_60.799495"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="actP"
                     /locus_tag="PLH85_16880"
     CDS             complement(<1..965)
                     /gene="actP"
                     /locus_tag="PLH85_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361867.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNB66293.1"
                     /translation="MRTSKLILGLLAVLGSGAALAAGADLGQAEKQATNWTAIIMFGV
                     FVAFTMFITKWAAGKTKSAADFYTAGGGISGFQNGLAIAGDYMSAASFLGISAAVMAN
                     GYDGLIYAIGFLVGWPIITFLMAERLRNLGKFTFADVAGYRFQQTPIRAFAASGTLVV
                     VAFYLIAQMVGAGQLIKLLFGLEYWVAVVVVGALMMVYVLFGGMTATTWVQIIKACLL
                     LAGASFMAFMVMSKFGFSPEAMFAKSVEIKTALAAKDAKLLEEAAKVAAEKGKEVTEV
                     VAAMAAKKGDSIMGPGSFVKDPISAISFGMALMFGTAGLPHILMR"
     gene            complement(965..1273)
                     /locus_tag="PLH85_16885"
     CDS             complement(965..1273)
                     /locus_tag="PLH85_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNB66294.1"
                     /translation="MKDDLGDRIRANPKYQELVSKRNSYGWIMTILMLAVYYGYILLV
                     AFNKEFLAQKLGAGVMSLGIPIGVGVILFTILITGIYIRRANTEFDDLKAQVIKEATK
                     "
     gene            complement(1479..>2828)
                     /gene="groL"
                     /locus_tag="PLH85_16890"
     CDS             complement(1479..>2828)
                     /gene="groL"
                     /locus_tag="PLH85_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB66295.1"
                     /translation="REGMKFVAAGMNPMDLKRGIDKAVAATLEELKKLSKPCSTTKEI
                     AQVGSISANSDASIGDRIAEAMEKVGKEGVITVEDGKSLADELDVVEGMQFDRGYLSP
                     YFINNPDKQVAILDAPFVLLFDKKISNIRDLLPVLEQVAKAGRPLLIIAEDVDGEALA
                     TLVVNNIRGILKTCAVKAPGFGDRRKAMLEDIAILTGGQVISEEVGLNLEKATLQDLG
                     QAKRIEVGKENTTIIDGAGNAERIEARVKQIRVQIEEATSDYDREKLQERVAKLAGGV
                     AVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALLRARASLAGLKGDNH
                     DQDAGIKIVLRAMEQPLREIVGNAGDEPSVVVNKVVEGSGNFGYNAASGEYGDMVEMG
                     VLDPTKVTRTALQNAASVAGLMLTTDCMVAELAEDKPAAGMPGMGGMGGMGGMDMGM"
BASE COUNT          507 a          861 c          924 g          536 t
ORIGIN      
        1 aagcgcatca ggatgtgggg cagaccggcg gtaccgaaca tcagggccat gccgaaggag
       61 atcgcggaga tcggatcctt cacgaaggaa cccggaccca tgatggaatc acccttcttg
      121 gcggccatgg cggcgaccac ttcggtgact tccttgccct tctcggcggc gaccttggcg
      181 gcttcctcga gcagcttggc atccttggcg gccagcgcag tcttgatctc gaccgacttg
      241 gcgaacatcg cttcggggct gaagccgaac ttggacatca ccatgaaggc catgaaggaa
      301 gcaccggcga gcagcaggca ggccttgatg atctgcaccc aggtggtggc ggtcatgcca
      361 ccgaagagca cgtagaccat catcagcgcg ccgacgacca ccaccgcgac ccagtactcg
      421 agaccgaaca gcagcttgat gagctgaccg gcaccgacca tctgggcgat gaggtagaag
      481 gccacgacga ccagcgtgcc ggacgccgca aaggcgcgga tcggggtctg ctggaagcgg
      541 tagccggcca cgtcggcgaa ggtgaacttg cccaggttgc gcaggcgttc ggccatcagg
      601 aaggtgatga tcggccagcc aaccaggaag ccgatggcgt agatcaggcc atcgtaaccg
      661 ttggccatca ctgcggcgga aatacccagg aaggacgcgg cggacatgta gtcgccggcg
      721 atcgcgaggc cgttctggaa gccggagatg ccaccgccgg cggtgtaaaa gtcggccgcg
      781 gacttggtct tgccggcggc ccacttggtg atgaacatcg tgaaggcgac gaacacgccg
      841 aacatgatga tggcggtcca gttggtcgcc tgcttttcgg cctggccgag gtcggcaccg
      901 gccgccagag cagcgccgga acccagcacg gccagcaggc cgaggatcag tttggaagtg
      961 cgcatcactt ggtggcctcc ttgatgacct gagccttcag gtcgtcgaat tcggtgttgg
     1021 cccggcggat gtagatgccg gtgatcagga tcgtgaacag gatcacgccc acgccgatgg
     1081 gaatcccgag ggacatgacg ccagcgccca gcttctgggc caggaattcc ttgttgaagg
     1141 ccaccagcag gatgtagccg tagtacacgg ccagcatcag gatcgtcatg atccagccgt
     1201 acgaattgcg tttggaaacc agctcctggt acttgggatt tgcccggatg cggtcgccga
     1261 ggtcgtcttt catctctctc ctcctctata aaaccttgtt aggacccgat aagactcctt
     1321 taagggtagg ggcaaggtac cgaccatccc ttactgtgaa cttacgtggg cgtcagtttg
     1381 agagaacacc tcaagcgtca gcgcgaaatc cgcgcagcca aaagaaaaac cccgccgaag
     1441 cggggttttt ctcagggcct gaagcgggcc ggacgggatt acatccccat gtccatgccg
     1501 cccatgccac ccatgccgcc catgccgggc atgccggcgg cgggcttgtc ttcggccagc
     1561 tcggcaacca tgcagtcggt ggtgagcatc aggccggcga cggaggcggc gttctgcagc
     1621 gcggtgcggg tcaccttggt gggatccagc acgcccatct cgaccatgtc gccgtactcg
     1681 ccggaggcgg cgttgtagcc aaagttgccg gaaccttcga caaccttgtt gaccaccacg
     1741 gagggctcgt caccggcatt gccgacgatc tcgcgcaggg gctgttccat ggcgcgcagc
     1801 acgatcttga tgccggcatc ctggtcgtgg ttgtcaccct tgaggccggc caggctggca
     1861 cgggcacgca gcagggcgac gccgccgccg gggacgatgc cttcttcaac ggcagcacgg
     1921 gtggcgtgca gcgcgtcttc aacacgggcc ttcttttcct tcatttcgac ttcggtcgcg
     1981 gcaccgacct tgatcacggc cacgccgccg gccagcttgg cgacgcgctc ctgcagcttc
     2041 tcgcggtcgt agtcggaggt ggcttcctcg atctgcacgc ggatctgctt gacgcgggct
     2101 tcgatgcgct cggcgttgcc ggcaccgtcg atgatggtgg tgttttcctt gcccacttcg
     2161 atgcgcttgg cctggcccag gtcctgcagg gtggcctttt cgaggttcag gccgacttct
     2221 tcggagatca cctggccacc ggtcaggata gcgatgtctt ccagcatggc cttgcggcgg
     2281 tcaccgaagc ccggcgcctt gacggcacag gtcttgagaa tgccgcggat gttgttcacc
     2341 accagggtgg ccagcgcttc gccgtccacg tcttcggcga tgatcagcag cgggcggccg
     2401 gccttggcga cctgttccag caccggcagc agatcgcgga tgttggagat cttcttgtcg
     2461 aacagcagga cgaagggggc gtccaggatg gcgacctgct tgtccgggtt gttgatgaag
     2521 tagggggaca ggtagccgcg gtcgaactgc atgccttcca ccacgtcgag ctcgtcggcc
     2581 agggacttgc cgtcttcgac ggtgatcacg ccttccttgc cgaccttttc catggcttcg
     2641 gcgatgcggt caccgatgga cgcgtcggag ttggcggaga tggagccgac ctgggcgatt
     2701 tccttggtgg tggagcaggg cttggagagc ttcttcagct cttccagagt ggcggcgacg
     2761 gccttgtcga tgccgcgctt gaggtccatc gggttcatgc cggcggcgac gaacttcatg
     2821 ccttcgcg
//
LOCUS       DAOQMM010000183         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_27257_length_2762_cov_41.039158, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000183 DAOQMM010000000
VERSION     DAOQMM010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27257_length_2762_cov_41.039158"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLH85_16895"
     CDS             <1..537
                     /locus_tag="PLH85_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HNB66296.1"
                     /translation="ASIATTWALVLASILLYLPANLLPMMITASFLGSQEDTILSGVI
                     FLWESGSWPLAVVVFFASVMVPLLKILALLYLLISVQRRSRRHPLQRTRLYRVVEFVG
                     RWSMLDIYVITLLVALVHFQGLATVQAGPAAVAFGAVVVLTMFAALSFDPRLIWDPLA
                     DATPPSTPPSPAVPDRHD"
     gene            530..2176
                     /locus_tag="PLH85_16900"
     CDS             530..2176
                     /locus_tag="PLH85_16900"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNB66297.1"
                     /translation="MTEPTAPPPPEIPVATVEAPRRFSIPLVWIIPLVAALIGLFLAA
                     RSYYEQGPTITIRFATGEGLEPGKTRIKYKDVDVGQITAVALDEDGRKVIASARVARQ
                     AERLLVDDTRFWVVSARVSGSSVSGLNTLFSGAYVGLDAGKSDEVRRAFDALDAPPVI
                     SMDVPGTAFVLDAPSLNSITVGTPVYYRRIEAGQVTGYALADNGRRLEIRIFVKAPYD
                     RFVTADSRFWNASGVDVKLGADGVQVNTESLTSILAGGLAFLTPESASDEPAPKDTRF
                     RLYNSREEATKQAGQNAQRYLLRFGESVRGLSVGAPLDFRGIAVGEVTAIHADLGTQG
                     SEMGMLVEVAVFPGRMLPRPDAVKPTFIGKDYRDKDFRAYFDKLIAGGLRAQLRSGNL
                     VTGQLYVALDFFPNARPGKVDWAATPPLIPTQRGSLEELQNTLMRLVAKLDRIPVEDI
                     GRDTHKAIVSLGRTVDELEQLIQRMDGLMQGDARSTLADARAAIADARQLLAADAPMQ
                     QDLRASLQELTRAATALRALADTLERTPESLLRGKSQDTP"
     gene            2173..>2762
                     /locus_tag="PLH85_16905"
     CDS             2173..>2762
                     /locus_tag="PLH85_16905"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HNB66298.1"
                     /translation="MTRSHRLHRSAPALAALLLSAALAACSSSPPVRHYSLGPLAHSA
                     GDASAAPPDSGAGPSLIVGPVSLPDVIDRPQIVRIVDGVRVEVADTHRWSGNLRNEIA
                     RRLATGIAHDRHLGRVVAWPASSVVQPDYTVPVDIQRLETTAFDHVTLEAVWTLRHAG
                     KDVAGGRFVATEKIEGGTYEGLAAAHGRLVDALARE"
BASE COUNT          423 a         1139 c          809 g          391 t
ORIGIN      
        1 gccagcatcg ccaccacctg ggcgctggtg ctggcctcca tcctgctgta tctgccggcc
       61 aacctgctgc ccatgatgat caccgcgtcc ttcctgggca gccaggaaga cacgatcctg
      121 agcggcgtga tcttcctgtg ggaatccggc tcgtggccgc tggcggtggt ggtgttcttt
      181 gccagcgtga tggtgcccct gctcaagatc ctggccctgc tctatctgct gatcagcgtg
      241 cagcgccgct cgcgccgcca tccgctgcaa cgcacgcgcc tgtatcgcgt ggtggaattc
      301 gtcgggcgct ggtccatgct ggacatctac gtgatcaccc tgctggtggc gctggtgcac
      361 ttccagggcc tcgccacggt gcaggccggc cccgccgcag tggcctttgg cgcggtggtg
      421 gtgctcacca tgttcgccgc cctgagcttc gacccgcgcc tgatctggga tcccctcgcc
      481 gacgcgacgc cgccctccac accgccttcc cccgccgtgc ccgaccgcca tgactgaacc
      541 caccgcgccg ccgcctcccg agatccccgt ggccaccgtc gaggcgccgc gccgcttcag
      601 cattccgctg gtgtggatca tcccgctggt ggcggcgctg atcggtctct tcctcgccgc
      661 ccgcagctat tacgaacaag gccccaccat caccatccgc tttgccaccg gcgaaggcct
      721 ggagccgggc aagacgcgca tcaagtacaa ggacgtggac gtgggccaga tcaccgccgt
      781 ggccctggac gaagacggcc gcaaggtgat cgccagcgcg cgtgtggcgc gtcaggccga
      841 acgtctgctg gtggacgaca cgcgcttctg ggtggtcagc gcccgcgtct cgggcagctc
      901 cgtgtccggc ctcaacaccc tcttctccgg cgcctacgtg gggctggacg cgggtaaatc
      961 cgacgaagtg cgccgcgcct tcgacgccct cgacgcgccg ccggtgatca gcatggacgt
     1021 gcccggcacc gccttcgtgc tcgacgcccc cagcctcaac tccatcaccg tgggcacccc
     1081 cgtgtattac cggcgcatcg aggccggcca ggtcaccggc tacgccctcg ccgacaacgg
     1141 ccgccggctg gagatccgca tcttcgtcaa ggcgccctac gaccgcttcg tcaccgccga
     1201 cagccgcttc tggaacgcca gcggcgtgga cgtgaagctc ggcgccgacg gcgtgcaggt
     1261 gaacaccgaa tccctcacct ccatcctcgc cggcggcctc gccttcctca cgccggagag
     1321 cgccagcgac gagcccgccc ccaaggacac ccgcttccgc ctctacaaca gccgcgaaga
     1381 agccaccaag caggccggcc agaacgccca gcgctacctg ctgcgctttg gcgaatcggt
     1441 gcgcggcctg tcggtgggcg cgccgctgga tttccgcggc atcgccgtgg gcgaagtcac
     1501 cgccatccac gccgacctgg gcacccaggg cagcgagatg ggcatgctcg tggaagtggc
     1561 cgtcttcccc ggccgcatgc tgccccgccc ggacgccgtc aaacccacct tcatcggcaa
     1621 ggactaccgc gacaaggact tccgcgccta cttcgacaag ctcatcgccg gcggcctgcg
     1681 cgcccagctg cgcagcggca acctcgtcac cggccagctc tacgtggccc tggacttctt
     1741 ccccaacgcc cgccccggca aggtggactg ggccgccaca ccgcccctca tccccaccca
     1801 gcgcggcagc ctggaagaac tccagaacac cctcatgcgc ctcgtcgcca agctcgaccg
     1861 catccccgtg gaagacatcg gccgcgacac ccacaaagcc atcgtcagcc tcggccgcac
     1921 cgtggacgag ctcgaacagc tcatccagcg catggacggc ctgatgcagg gcgacgcccg
     1981 cagcaccctg gccgacgccc gcgccgccat cgccgacgca cgccagctcc tcgccgccga
     2041 cgccccgatg cagcaggatc tgcgcgcctc cctgcaggag ctcacccgcg ccgccacggc
     2101 cctgcgcgcc ctcgccgaca ccctcgaacg cacacccgaa tccctgctcc gcggcaaatc
     2161 ccaggacacg ccatgacccg ctcccaccgc ctgcaccgca gcgcccccgc cctcgccgcc
     2221 ctcctcctga gcgccgccct cgccgcctgc agcagctcgc cccccgtccg ccactacagc
     2281 ctcggccccc tcgcccacag cgccggcgac gcctccgccg caccaccgga cagcggcgcc
     2341 ggccccagcc tcatcgtcgg ccccgtcagc ctgccggacg tcatcgaccg cccccagatc
     2401 gtccgcatcg tggacggcgt gcgcgtcgaa gtggccgaca cccaccgctg gagcggaaac
     2461 ctgcgcaacg aaatcgcccg ccgcctcgcc accggcatcg cccacgaccg ccacctcggc
     2521 cgcgtcgtcg cctggcccgc cagcagcgtc gtccagcccg actacaccgt ccccgtagac
     2581 atccagcgcc tcgaaaccac cgccttcgac cacgtcaccc tcgaagccgt ctggaccctc
     2641 cgccacgccg gcaaagacgt agccggcgga cgcttcgtcg cgacggagaa gatcgaaggc
     2701 gggacctacg aaggcctcgc cgcagcccat ggacggctgg tggatgcgct ggcgcgggag
     2761 at
//
LOCUS       DAOQMM010000184         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_28753_length_2676_cov_55.173216, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000184 DAOQMM010000000
VERSION     DAOQMM010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28753_length_2676_cov_55.173216"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLH85_16910"
     CDS             complement(<1..437)
                     /locus_tag="PLH85_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB66299.1"
                     /translation="MSYLSINNIEVIYDHVILVLKGVSLEVPQGKIVALLGANGAGKS
                     TTLKSISNLLRAERGDVTKGSIEFKGDRVDCMTPADLVKRGVIQVMEGRHCFGHLSIE
                     ENLLTGAYTRSLSRSALKENLERVYAYFPRLKTRRTSQAGYTSG"
     gene            complement(635..1957)
                     /locus_tag="PLH85_16915"
     CDS             complement(635..1957)
                     /locus_tag="PLH85_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB66300.1"
                     /translation="MKLKRGFLAGLMAAGLLGSGAAVADEQFIGLPSYRVGPYAAGGS
                     GLFGGWIDYMQMINERDGGINGVKLTWEECETEYNNARGVECYERLKKKGSTGNSVFQ
                     PVSTGITYSVLEKVAQDKIPMVTIGYGRTDAADGRVFPWVFPLITTYWSQASAIVNYI
                     GSLEGGMDRLKGKKIGLLYHDSAYGKESHAILDKLAAKHGFEVTKIAVAHPGNEQQSQ
                     WLQIRQLKPDYVILWGWGVMNPTAIKAAAKTGFPREKLIGVWWSGAEEDTIPAGPAAK
                     GFIAAGFNVAGANYPVIADIKKHVYGKSKGNMEDKTRIGSIYYNRGVVHGLITVEAIR
                     KAQDKFGKGKALTGEQVRWGFENLNLDDARLKALGATGFLPPLKISCADHEGPGKVKF
                     QQWDGSQWKVISDWVESDRPLVRGMIEESAMAYAKEKGIKPRDCSKEN"
     gene            complement(2020..>2676)
                     /locus_tag="PLH85_16920"
     CDS             complement(2020..>2676)
                     /locus_tag="PLH85_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB66301.1"
                     /translation="LAAQFFIVWALVKFPWFSNNSSSGVVTAQDVTILGYTFVSPESK
                     YVLTFLIVAAMALMAKNLVRSATGRAWMAVRDMDVAAQVIGFRLMRTKLLAFAVSAFY
                     CGVAGALYAYTYIGTVEPEGFNLDLSFKILFMIIIGGVGSILGSFLGAAFIVLLPILL
                     DNLVPMLFGNALGAGFVSNFQLIVFGGLIIFFLIVEPHGLARLWQIAKEKLRLWPFPH
                     "
BASE COUNT          441 a          864 c          896 g          475 t
ORIGIN      
        1 cccgaggtat agccggcctg ggacgtgcgt cgcgtcttga ggcgcggaaa gtaggcgtac
       61 acccgctcca ggttctcctt cagcgcgctg cgggacaggc tgcgggtgta ggcgccggtg
      121 agcaggttct cctcgatgga caggtgaccg aagcagtggc ggccctccat cacctggatc
      181 accccgcgct tcaccagatc ggccggcgtc atgcagtcca cccggtcgcc cttgaactcg
      241 atgctgccct tggtcacatc gccccgctcg gcccgcagca ggttggagat ggacttgagc
      301 gtcgtgctct tgccggcccc gttggcgccc agcaaggcca cgatcttccc ctggggcacc
      361 tccagggaga cgcccttgag cacgaggatc acgtggtcgt agatgacctc gatgttgttg
      421 atgctgaggt aagacatggc gtcggccatc cccctgtgcg gacggctggg gccgtccggg
      481 ttccggttgc gataagaaag cagtcaccca aggttcggtg cgcgcacgcg tcacgcgcgg
      541 ctcgaggccg tggcgggacg cgcagccgta cgggcgtacg gcgggcctca gcgccgcggg
      601 atcggcccgc gcagccgcgt gcgtgccgcc cccgtcaatt ttccttggag cagtcgcggg
      661 gcttgatgcc cttttccttg gcgtaggcca tggccgattc ctcgatcatg ccgcgcacca
      721 gaggccggtc ggattccacc cagtcggaga tcaccttcca ctggctcccg tcccactgct
      781 ggaacttcac cttgcccggc ccttcgtgat cggcgcagct gatcttgagg ggcggcagga
      841 agccggtggc gcccagggcc ttcaggcggg cgtcgtccag attgaggttc tcgaagcccc
      901 agcgcacctg ctcgccggtg agggccttgc ccttgccgaa cttgtcctgg gccttgcgga
      961 tcgcctcgac ggtgatgagc ccgtgcacca cgccccggtt gtagtagatg gagccgatgc
     1021 gggtcttgtc ttccatgttg cccttgctct tgccgtacac atgcttcttg atgtcggcga
     1081 tcacggggta gttggcgccg gccacattga agccggcggc gatgaagccc ttggcggccg
     1141 ggccggcggg gatggtgtcc tcttcggcac cggaccacca cacgccgatc agcttctcgc
     1201 gggggaagcc ggtcttggcg gcggccttga tggcggtcgg gttcatcacg ccccagcccc
     1261 acaggatcac gtagtccggc ttgagctggc ggatctgcag ccactgggac tgctgctcgt
     1321 tgcccgggtg ggccacggcg atcttggtca cctcgaagcc gtgcttggcg gccagcttgt
     1381 cgaggatggc gtgggactcc ttgccgtagg cggagtcatg gtagagcagg ccgatcttct
     1441 tgcccttcag ccggtccatc ccgccctcca gcgagccgat gtaattgacg atggccgagg
     1501 cctgggacca gtaggtggtg atcagcggga acacccacgg gaagacgcgg ccatcggcgg
     1561 cgtcggtgcg gccgtagccg atggtgacca tggggatctt gtcctgggcc accttttcca
     1621 gcaccgaata ggtgatgccc gtggacaccg gctggaacac cgaattgccg gtgctgccct
     1681 tcttcttcag gcgctcgtag cactccacgc cgcgggcgtt gttgtactcc gtctcgcact
     1741 cttcccaggt gagcttgacg ccgttgatgc cgccgtcccg ctcgttgatc atctgcatgt
     1801 agtcgatcca gccgccgaac aggcccgagc cgcccgccgc gtaggggccg acccggtagc
     1861 tgggcaggcc gatgaactgc tcgtccgcca cggccgcgcc ggaaccgagc aggccggccg
     1921 ccatcaggcc ggcgagaaaa ccgcgcttca gtttcattgt tgtgtctcct cttgtaatgg
     1981 tgtggatgct gcaaccgccg gccgattgta cgcgcgcgct cagtggggga aaggccacag
     2041 gcggagcttc tccttggcga tctgccacag acgggccaga ccgtggggct cgacgatcag
     2101 gaagaagatg atgaggccgc cgaagacgat gagctggaag ttggacacga aacccgcgcc
     2161 gagggcattg ccgaagagca tcgggaccag gttgtccagc aggatgggca gcagcacgat
     2221 gaaggcggcg ccgaggaagg acccgaggat cgagcccacc ccgccgatga tgatcatgaa
     2281 caggatcttg aaggacaggt ccaggttgaa gccctccggc tccaccgtgc cgatgtaggt
     2341 gtaggcgtag agggcgcccg ccacgccgca gtagaaggcg ctcaccgcga aggccagcag
     2401 cttggtgcgc atcaggcgga aaccgatcac ctgggccgcc acgtccatgt cacgcaccgc
     2461 catccacgcc cgcccggtgg ccgagcgcac caggttcttg gccatcaggg ccatcgccgc
     2521 aacgatcagg aaggtgagca cgtacttgga ttccggcgac acgaaggtgt agccgaggat
     2581 ggtcacgtcc tgggccgtca ccacgccgga ggacgagttg ttggagaacc acggaaactt
     2641 caccagcgcc cacacgatga agaactgggc cgccag
//
LOCUS       DAOQMM010000185         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_32058_length_2518_cov_28.855867, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000185 DAOQMM010000000
VERSION     DAOQMM010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32058_length_2518_cov_28.855867"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="PLH85_16925"
     CDS             complement(<1..1567)
                     /locus_tag="PLH85_16925"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNB66302.1"
                     /translation="MGPVLHLEADHPVDAVVQLIRSRLGDDAPQAEAFTRQFYSQVAL
                     DDLQGRPVQDLYGAALSHWHFARRFSGGAPRLRVYNPRPEEHGWQSTHTVIEIVSEDM
                     PFLVDSITMEVNRQGLTLHLIIHPLMRTRRDTDGTLQAVAADTADEAAGRLESIIHVE
                     VDRRSEAADLDALGAGLTHILADVRAAVGDWPVMRERLAAAIADVRSAPLPIDDAERD
                     EGIAFLEWLAADNFTLLGCREYELANEEGDDVLRVVPGSGAGILRESGKERSLAFAAL
                     PPEVRTVAREPHLLIITKANGRSTVHRPGYMDYIGVKRFGPDGSVTGERRFLGLFTST
                     AYNISPLAIPLLRRKVETVQRLAALRPQSHAGRALLTILEQYPRDELIQSSAAELHAT
                     AMGILGLGERQRTRLFMRRDPFGRFICCLIYAPRETYDTGMRQRMQNVLMDTLGGVSS
                     EFNVQLSESALARILIVVHTPPGTVRSFDVRDLELRLVRATRRWEGDLSDALRERCGE
                     ERGNFLNARYRNAF"
     gene            1855..>2518
                     /locus_tag="PLH85_16930"
     CDS             1855..>2518
                     /locus_tag="PLH85_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNB66303.1"
                     /translation="MENSLVVAVAQLNCVVGDLSGNARRILEAVSRAQAAGADVVLTP
                     ELALCGYPPEDLLLRPAFLDACAAELDALAAQVQGVTVLVGHPLAADGLRYNAASVIR
                     DGRVVATYRKHRLPNYEVFDEERYFEAGTEACVFEHKGVRIGVNICADVWEAGAADVA
                     RGAGAELLLVLNASPYHMNKQQRRYEVMRERIADTGLPVVYCNLVGGQDELVFDGGSF
                     ALD"
BASE COUNT          344 a          777 c          983 g          414 t
ORIGIN      
        1 ggaaggcgtt gcggtagcgg gcgttgagga agttgccgcg ctcctcgccg cagcgttccc
       61 gcagtgcgtc ggagaggtcg ccctcccagc gccgggtggc gcgcaccagg cgcagctcca
      121 ggtcgcgcac gtcaaagctg cgcacggtgc ccggcggggt gtgtacgacg atgaggatgc
      181 gggcgagggc ggattcggaa agctggacgt tgaactccga gctgacgccg ccgagagtgt
      241 ccatcagcac gttctgcatg cgctggcgca tccccgtgtc gtaggtctcg cggggcgcgt
      301 agatgaggca gcagatgaag cgcccgaagg ggtcgcgacg catgaagagg cgggtgcgct
      361 ggcgctcacc gaggcccagg atgcccatcg cggtggcgtg cagctcggcg gcgctgctct
      421 ggatgagctc gtcacgcggg tattgttcga gaatggtgag gagggcgcgg ccggcgtggc
      481 tttgcgggcg cagggcggcc agtcgctgga cggtctcgac cttgcgccgc agcagcggga
      541 tggcgagggg gctgatgttg taggcggtgg aggtgaacag gcccaggaag cgccgctcgc
      601 ccgtcacgct gccgtccggc ccgaagcgct tgacgccgat gtagtccatg tagccggggc
      661 ggtgcacggt ggagcggccg ttggccttgg tgatgatcag caggtggggc tcgcgggcga
      721 cggtgcgcac ctccggcggc aaggcggcga aggcgaggga gcgctccttg ccggattccc
      781 gcaggatgcc ggcgccggag ccgggcacca cgcgcagcac gtcgtcgccc tcttcattgg
      841 cgagttcgta ctcgcggcac ccgagcaggg tgaagttgtc ggcggcgagc cattcgagga
      901 aggcgatgcc ctcgtcccgc tcggcgtcgt cgatgggcag gggcgcgctg cgcacgtcgg
      961 cgatggccgc ggcgaggcgc tcgcgcatga cgggccagtc accgacggcg gcacgcacgt
     1021 cggcgaggat gtgggtgagg ccggcgccga gggcgtccag gtcggcggcc tcggagcggc
     1081 gatccacctc cacgtggatg atggactcga ggcgcccggc cgcttcgtca gcggtgtctg
     1141 cggcgacggc ttgcagggta ccgtcggtat cccggcgggt gcgcatcagc gggtggatga
     1201 tgaggtgcag ggtcaggccc tgacggttca cctccatggt gatggagtcc accaggaagg
     1261 gcatgtcctc gctgacgatc tcgatcacgg tgtgggtgga ttgccagccg tgctcctcgg
     1321 gccgggggtt gtacaccctg aggcgcggcg cgcctccgct gaagcggcgg gcaaaatgcc
     1381 agtgggacag ggcggcgccg tagaggtcct gcactggccg gccctgcagg tcgtccaggg
     1441 ccacctggct gtagaactgg cgggtgaagg cttccgcctg gggcgcgtcg tcgcccagac
     1501 ggctgcggat gagctggacg acggcgtcca caggatggtc ggcttcgaga tggaggacgg
     1561 ggcccatgat gtctcctcgt ggggtgtcgc gttgggcgac ggttattatg gtcctgcgcg
     1621 ggcgcggcgg ccggcggatc gcccggacgt ccgtgggcgg atttacggtc gcgccggggt
     1681 gcttcctgag ggacgtccgt gagaagtgtc acgggcgtgg gttctcccct ttcattttag
     1741 cgggtttccg gcggcgggca aaggcgccgg tggatggcgc gacgcgacgg gcggcgggcc
     1801 ttccgcgtgc ctgcggattc tgccgcggcg ggccgtccgg tatagtgtcg ccccatggaa
     1861 aacagtcttg tcgtcgcagt cgcccagctc aattgcgtgg tgggcgatct ttccggcaat
     1921 gcccgccgga ttctcgaagc ggtgtcccgc gcccaggcgg ccggggccga tgtggtgctc
     1981 accccggaac tggccctgtg cggctatccg ccggaagacc tgctgctgcg cccggccttt
     2041 ctcgatgcct gcgccgccga actcgatgcg ctggccgcgc aggtgcaggg cgtcacggtg
     2101 ctggtggggc atccgctggc cgcggacggc ctgcgctaca acgccgcctc ggtgatccgc
     2161 gacgggcgcg tcgtcgccac ctaccgcaag catcgcctgc ccaactacga ggtgttcgac
     2221 gaggagcgct atttcgaggc cggcaccgag gcctgcgtct tcgagcacaa gggcgtgcgc
     2281 atcggcgtga acatctgcgc cgacgtgtgg gaagcggggg ccgccgacgt ggcccgtggc
     2341 gccggtgcgg agctcctgct ggtgctcaac gcgtcgccct atcacatgaa caagcagcag
     2401 cgccgctatg aggtgatgcg cgaacgcatc gccgataccg gcctgccggt ggtctattgc
     2461 aatctggtgg gcgggcagga cgagctggtc ttcgacggcg gctccttcgc cctcgacc
//
LOCUS       DAOQMM010000186         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_32084_length_2517_cov_35.488627, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000186 DAOQMM010000000
VERSION     DAOQMM010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32084_length_2517_cov_35.488627"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLH85_16935"
     CDS             complement(<1..460)
                     /locus_tag="PLH85_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB66304.1"
                     /translation="MDKARFVEIVRDTPLVSVDLVVLRDHREVLLGLRTNRPAQGTWF
                     VPGGRVLKNERRADALQRVAQRELGIADLHALAPEFLGPFEHFYPDCFAGDNGVSTHY
                     VVLAHLIHVPAGFEIPGQDDQHEALRWWPLDEADAEPAVHRFTRDYLPLLR"
     gene            780..908
                     /locus_tag="PLH85_16940"
     CDS             780..908
                     /locus_tag="PLH85_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66305.1"
                     /translation="MTCIRLSFRRPAGFARISRHAPALRPDVRVPVPPSPPPSSPC"
     gene            922..1836
                     /locus_tag="PLH85_16945"
     CDS             922..1836
                     /locus_tag="PLH85_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB66306.1"
                     /translation="MSVPLAYLSIVLIWSTTPLAIQWSTQGAGYALAVFARMLIGVSV
                     AALLMALWRIPLPFHARARRAYLSGGIGLFGAMALTYWGARFVNSGLVSVLFGLSPLI
                     AGGLASVWLGEDALTRPKLAGTLLGAAGLAVIFVHGGSVGGEHAVAGLAALLAAVCVY
                     SASLVSLKRIGDDSPPLATTVGTLAVSLPGFGLMWWLGDGHLPAELPARTGAAIAYLG
                     VFGSVIGFALYYYVIQHMDASKVALITLITPVMALLLGHGLNGEALGPRVWIGTGLIL
                     AGLGVYQDLPGRFRLRPAPDTDGARRQP"
     gene            complement(2088..>2517)
                     /locus_tag="PLH85_16950"
     CDS             complement(2088..>2517)
                     /locus_tag="PLH85_16950"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB66307.1"
                     /translation="GVLAHEIAHVELRHSLRQLVRSAGLQVIAAALFGDYAGLGQLGA
                     QLGALKFSRDAEREADARGLQRLADAHIDPQGLLRFFETLADTEGMAATMPAVLSTHP
                     ATAERIDALKRALAAHPAEAVTPLAVSWPAVHAALDKTAR"
BASE COUNT          324 a          930 c          851 g          412 t
ORIGIN      
        1 cccggagcag cggcaggtaa tcccgcgtga agcgatgcac cgccggctcc gcatccgcct
       61 catcgagggg ccaccagcgc agggcctcgt gctgatcgtc ctgcccgggg atctcgaagc
      121 cggcgggcac gtggatcagg tgagcgagca ccacgtaatg ggtgctcaca ccgttgtcgc
      181 cggcgaagca gtctggatag aaatgctcga aggggcccag gaattccggc gcgagggcgt
      241 ggaggtcggc aatgcccagt tcccgctgcg ccacccgctg cagggcatcc gcgcgccgct
      301 cgttcttgag gacgcgcccg ccgggtacga accaggtgcc ttgggccggc cggttggtgc
      361 gcaggccgag cagcacctcc cgatggtccc ggagcactac cagatccacc gacaccagcg
      421 gcgtgtcccg aacgatctcc acgaatcgtg ccttatccat cctgtgaatc ctccttgtcc
      481 attgaaaatc cgcgccaccc catccattgg ccgaaccagc cttgacgcga cgcacaaacg
      541 accgggagaa taccgcaatg gacctcatcc agtcggcatc ccccgccgcc ttcctgccga
      601 tcatccgact gcgccgtgcg ctgcgcgcgt ttgcgcgtgc accgcggccg ggcgggcgcc
      661 gaacggactg atgtcccgaa cgccccgaat tcccgacagg ccgcgcttca ccccgcggcc
      721 tgtttcattt tcccccctac ccgactgtcg tctcttttcc tgaccgtgga gatctgatca
      781 tgacctgcat ccgcctgagt ttccgccgcc cggccggctt tgcccgcatc tcccgccacg
      841 ctccggccct gcgcccggac gtgcgcgtcc ccgtgccgcc ttctccgccg ccctcgtccc
      901 cctgttgatc cggagcaccc catgtccgtc cccctcgcct atctcagcat cgtcctgatc
      961 tggtctacca cgcccctggc catccagtgg agcacccagg gcgccggtta cgccctggcc
     1021 gtattcgccc gcatgctcat cggcgtcagc gtcgcggcgc tgttgatggc gctgtggcgc
     1081 attcccctgc ccttccatgc ccgcgcgcgg cgggcctacc tgtccggcgg catcgggctc
     1141 ttcggggcca tggcgctcac ctactggggc gcgcgtttcg tcaattccgg gctggtgtcg
     1201 gtgcttttcg gcctgtcgcc cctcatcgcc ggcgggctgg cgagtgtctg gctgggcgag
     1261 gacgccctca cccgccccaa gctggcaggc acgctgctgg gcgcggcagg tctggcggtg
     1321 atcttcgtgc atggcggcag cgtgggcggt gagcatgcgg tggcgggcct cgccgcgctg
     1381 ctggcggcgg tctgcgtgta ttcggcgagc ctggtgtccc tcaagcgcat cggtgacgac
     1441 agcccgcccc tggccaccac ggtgggcacc ctggccgtgt ccctgcccgg cttcgggctc
     1501 atgtggtggc tgggcgacgg gcatctgccg gccgagctgc ccgcgcgcac cggcgccgcc
     1561 atcgcctacc tgggtgtctt cggttcggtg atcggctttg cgctgtacta ctacgtgatc
     1621 cagcacatgg acgcctccaa ggtggcgctg atcaccctga tcacgccggt gatggcgctg
     1681 ctgctgggtc acggcctgaa cggcgaggcg ctgggcccgc gggtgtggat cggcacgggg
     1741 ctgatcctgg cgggcctggg ggtgtatcaa gacctccccg gtcggttccg cctgcggccc
     1801 gccccagaca ccgacggggc gcggcgacag ccctgagccc ggcgtcgcgt gtcggatcgc
     1861 cggtccgccc ggccccgtgt ggtgacagca cagtcagtgc cgacggctca gggcccgcac
     1921 ggcgcccacc accgggtgca tgtccacgcc gggccgcagg cgcagcccgt cccgttgcga
     1981 atcccgggac aaggcgcggg aaagcctccg tcttcagggc gtcggcccat gtccgcacgc
     2041 gcatgcgggc gggcacgggg ggcggcgcgc cgaggcgacg gcggggctta ccgggcggtc
     2101 ttgtccaggg cagcgtgcac ggccggccag ctcaccgcca acggcgtcac cgcttcagcc
     2161 ggatgggccg ccagggcgcg cttgagggcg tcgatgcgtt ccgccgtggc cgggtgggtg
     2221 gacagcacgg cgggcatcgt cgccgccatg ccttccgtgt ccgccagggt ttcgaaaaag
     2281 cgcagcagac cctgcgggtc gatgtgcgca tcggccagcc gctgcagccc gcgggcgtcg
     2341 gcctcccgct ccgcgtcgcg ggagaacttg agcgcgccga gctgggcgcc cagttgcccc
     2401 aaccccgcat aatcgccgaa gagcgccgcg gcgatgacct gcaggccggc cgagcgcacc
     2461 aactggcgca ggctgtggcg cagctccacg tgggcgatct cgtgggccag cacgccg
//
LOCUS       DAOQMM010000187         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_33158_length_2468_cov_57.337339, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000187 DAOQMM010000000
VERSION     DAOQMM010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33158_length_2468_cov_57.337339"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLH85_16955"
     CDS             complement(<1..972)
                     /locus_tag="PLH85_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB66308.1"
                     /translation="MSEPQNPADNYDESSIQQLEGLEAVRKRPGMYIGDTSDGTGLHH
                     MVFEVVDNAIDEALAGHCDDIVITIHADNSISVTDNGRGIPTGVKMDDKHEPQRSAAE
                     IVMCVLHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLSVARDGKKHFMEFHRGAP
                     QNRVIEVVDGVETSPLQVVGETTKRGTKVHYLADEEIFGTVEYHYEILAKRLRELSFL
                     NNGVKIRLVDQRTGKEEDFAFAGGVSGFVDYINRAKTVLHPTVFHASGESLQNGVPIT
                     VEVAMQWNDSYAEQVLCFTNNIPQADGGTHLTGLRAAMTRVINKYIEE"
     gene            complement(1172..2278)
                     /gene="dnaN"
                     /locus_tag="PLH85_16960"
     CDS             complement(1172..2278)
                     /gene="dnaN"
                     /locus_tag="PLH85_16960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNB66309.1"
                     /translation="MLLFTAPRDALLAPLQSVSGIVEKRHTLPILSNVLIEKKGEALT
                     LLATDIEIQIRNRTTAALGAEDVAITVGARKLQDILRALPDSAEVQLTLDDKRITLKA
                     GKSRFQLQTLPAADYPRLAPPEGEGVHFSTTQKAFKKQLALVQYAMAQQDIRYYLNGL
                     LLVVAGGTLRMVATDGHRLAYAASAIEGDYPRTDVILPRKTVVELARQLADSDDPVEV
                     TLVGNQIVFRFGHIELISKLIDGKFPDYERVIPQHHPKLVKASRLALLQSLQRAAILT
                     NEKFRGVRLILSEGSLKLVSTNAEQEEAQEELEVDYSGDALDIGFNVTYLLDVLTNLS
                     ANEIEIRFNDNNASALFGLGDNPDFKYVVMPMRI"
BASE COUNT          347 a          633 c          982 g          506 t
ORIGIN      
        1 ttcttcgatg tacttgttga tgacgcgggt catcgcggcg cgcaggccgg tgaggtgggt
       61 gccgccgtcg gcctggggga tgttgttggt gaagcacagc acctgttcgg cgtagctgtc
      121 gttccactgc atggccactt cgacggtgat gggcacgccg ttctggaggg attcgccgct
      181 ggcgtggaag acggtggggt ggagcacggt cttggcgcgg ttgatgtagt ccacgaagcc
      241 ggagacgccg ccggcgaagg cgaagtcttc ttccttgccg gtgcgctggt ccacgaggcg
      301 gatcttgacg ccgttgttga ggaaggacag ttcgcgcagg cgcttggcga ggatttcgta
      361 gtggtattcg acggtgccga agatctcttc gtcggcgagg tagtggacct tggtgccgcg
      421 cttggtggtt tcgccgacga cctgcagggg gctggtctcg acgccgtcca cgacttcgat
      481 gacgcggttc tggggcgcgc ctcggtggaa ttccatgaag tgctttttgc cgtcgcgggc
      541 gacgctgagg cgcagccact tggagagggc attgacgcag gagacgccca cgccgtgcag
      601 gccgccggag accttgtagc tgttctggtt gaacttgccg ccggcgtgga gcacgcacat
      661 gacgatctcg gcggcggagc gctggggctc gtgcttgtcg tccatcttga cgccggtggg
      721 gatgccgcgg ccgttgtcgg tgacggagat ggagttgtcg gcgtggatgg tgatcacgat
      781 gtcgtcgcag tggccggcga gggcttcgtc gatggcgttg tccaccactt cgaagaccat
      841 gtggtgcagg ccggtgccgt cggaggtgtc gccgatgtac atgccaggcc gcttgcgcac
      901 ggcctccagc ccttcgagct gctggatgct ggattcgtcg tagttgtcgg cggggttttg
      961 cggttcggac atggtgttct cggttctgcg aaggcgtgct gcgcggcagc gttactgcgt
     1021 tggttcggga cgtaaacgac ccgggatcac gttcgtggag aaagcgtcgc gagcggttcg
     1081 aggtcgcact gaggcgcgga gctgtaccgg gcgtacagcg agcaccggaa ttgcgggatc
     1141 ggaccgcgcg gcagctttat ccacggacgc cttagatgcg catgggcatc acgacgtact
     1201 tgaagtcggg gttgtcgccc agcccgaaca gggcgctggc gttgttgtcg ttgaagcgga
     1261 tctcgatttc gttggcggag aggttggtga gcacgtcgag caggtaggtg acgttgaagc
     1321 cgatgtcgag ggcgtcgccg ctgtagtcga cttcgagctc ttcctgggct tcttcctgtt
     1381 cggcgttggt ggagacgagc ttgaggctgc cttccgagag gatcaggcgc acgccgcgga
     1441 acttctcgtt ggtgaggatg gcggcgcgtt gcagggactg gagcagggcg aggcggctgg
     1501 ccttgacgag cttgggatgg tgctggggga tgacccgctc gtagtcgggg aacttgccgt
     1561 cgatgagctt ggagatgagt tcgatgtgtc cgaagcggaa gacgatctgg ttgcccacca
     1621 gggtgacttc gacggggtcg tcggagtcgg cgagctggcg ggccagctcc acgacggtct
     1681 tgcgcggcag gatcacgtcg gtgcgggggt agtcgccttc gatggccgat gcggcgtagg
     1741 cgaggcggtg gccgtcggtg gcgaccatgc gcagggtgcc gccggcgacg accaggagca
     1801 ggccgttgag gtagtagcgg atgtcctgct gggccatggc gtactgcacc agggcgagct
     1861 gcttcttgaa ggccttctgg gtggtggaga agtgcacgcc ctcgccttcg ggcggggcca
     1921 ggcggggata gtcggcggcg ggcagggtct ggagctggaa gcggctcttg ccggccttga
     1981 gggtgatgcg cttgtcgtcg agggtgagct gcacctcggc gctgtcgggc agggctcgca
     2041 gaatgtcctg cagcttgcgg gcgcccacgg tgatggccac gtcctcggcg ccgagggcgg
     2101 cggtggtgcg attgcggatc tggatttcga tgtcggtggc gagcagggtc agggcttcgc
     2161 ccttcttctc gatcaggacg ttggagagga tcggcagggt atggcgcttt tcgacgatgc
     2221 cggaaacgga ctgcagcggg gcgaggaggg catcgcgggg tgcggtgaag agcagcatgt
     2281 gtcgggtctc cggatcgttg tcagcaagcg agtggcgttg tgggtgaagt cggggccgcg
     2341 gtcatccgcg caggacctgg gtgagcacgt gcaggtcgtg gttgagctgg gtgtcgccga
     2401 ggcgcagttc ggcgatggtg cggcaggcgt gcagcacggt ggtgtggtcg cggccgccga
     2461 aggcctcg
//
LOCUS       DAOQMM010000188         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_34570_length_2410_cov_33.419533, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000188 DAOQMM010000000
VERSION     DAOQMM010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34570_length_2410_cov_33.419533"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..621)
                     /locus_tag="PLH85_16965"
     CDS             complement(322..621)
                     /locus_tag="PLH85_16965"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNB66310.1"
                     /translation="MTTYIAFLRSGPDGACTVSFPDLPGCTVCGANAVLATALAGDEL
                     VRYLSVLLSLGEAAPPPTPEAALAQDPFRGDAALLAFDVDLEMLGIAHPLHFHTL"
     gene            complement(660..1034)
                     /locus_tag="PLH85_16970"
     CDS             complement(660..1034)
                     /locus_tag="PLH85_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HNB66311.1"
                     /translation="MVDRIVATPEAVDLLNRLRARHGPRLLFLQACGCCDHSSLYCYP
                     DTEYAVAPQDVLVGHIDGVPYYQDAQQHALFRHTQILIRVVPGDRSGDMSLEGTDGVI
                     FRLESRLFSADEIAALDARAAA"
     gene            complement(1314..>2410)
                     /locus_tag="PLH85_16975"
     CDS             complement(1314..>2410)
                     /locus_tag="PLH85_16975"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB66312.1"
                     /translation="SGSAFVLGMVGFASQAPILLFGAFGGVVTDRLDRRRVLLATQAL
                     SMVQALLLAALAWKDAATPGHLVGLAFVLGCINALDVPARQAIAVQLVDDPDDLPNAI
                     ALNSFLMNTARFVGPALAGFVVAEVGEAVCFLLNAASYLAVLMALRAIRLPAAGDRPP
                     APPPLQALREGWRYAMAHREIRASLRLVAAISLCAAPYVVLMPLFAKEEFGGGARLFG
                     LLVGSAGAGSLVASFYLAGRADTGGLAEKVAVAAPTVGVAVILFALSPGVGMAVPVLL
                     ALGGSIILTIAGSNTLIQTQVDNAFRGRVMALFSMAFLGVAPLGSFTVGSAAHVFGVR
                     PTLVVCGVLTVIAGLIYRRAVLRRKAAAAA"
BASE COUNT          392 a          893 c          857 g          268 t
ORIGIN      
        1 ggccggggcg ggggctggtc ggacgatggc aggactatac cgagggggcc cggctgaccc
       61 aagcaggggc gcacgggcgg cgccggcgca gcacatcgtg gccgccgatt atcgcggcgc
      121 attccggttg cgcgtggtgt gaacgcggcg gggccggagc ccggcgcggt cgtccggcga
      181 gggatgcgcg ggggcgtggg cccttcccgc gtcgtccccg tccactgcgc cggggccgcc
      241 ttcattcccg cgcggtgtag gggcggcccc cgcgggcacc acgcctgacg cgcaggccgc
      301 ccgcgcctgc ccggcgggcg gctacagggt gtggaagtgc aggggatggg cgatgcccag
      361 catctccaga tccacgtcaa aagccagcag ggccgcgtct ccgcggaacg ggtcctgggc
      421 cagggcggct tccggggtgg ggggcggtgc ggcttcgccg aggctcagca gcacgctcag
      481 gtagcgcacc agttcgtccc ccgccagcgc cgtggccagc acggcgttgg cgccgcagac
      541 ggtgcagccg ggcagatccg gaaagctcac ggtgcatgcg ccatccgggc cgctgcgcag
      601 aaaggcgatg taggtggtca tggtgtccgc ctcgttgaaa aggatcggca gcccgccgtt
      661 caggcggcgg cccgcgcgtc cagcgcggca atctcgtcgg cgctgaacag gcgcgattcc
      721 aggcggaaga tcaccccgtc ggtgccttcc agcgacatgt cgccgctgcg gtcgccgggc
      781 accacgcgga tcaggatctg ggtgtggcgg aacagggcgt gctgctgggc gtcctggtag
      841 tagggcacgc cgtcgatgtg gccgacgagc acgtcctggg gcgccaccgc gtactccgtg
      901 tccgggtagc aatacagcga gctgtggtcg caacagccgc aggcctgcag aaacagcagc
      961 cgcgggccgt gccgcgccct gagccggttc aaaagatcca cggcctccgg cgtggccacg
     1021 attcgatcca ccatgggggg ctcctccgct tgtggggcgc gcggccgggc gatggcagag
     1081 cccgcggcgc cggggtgggg acgtcccgcg gggtgccgaa cagcccggcc cgcgggtgga
     1141 ggacatcatg gaaggacgcg ccgcgtggca cgggacataa aagccgcaat tcgggtcatc
     1201 gtgaggggat gcacgccgcg gcgggccgcc cgcgtacggg ccgcagtggt ttgcgaaggg
     1261 gcctgcggag atcccgccgc gggctgcatc cgccccgccc ggcggcaaaa gcctcaggcc
     1321 gccgccgcgg ccttgcggcg cagcaccgcg cgccggtaga tcagcccggc gatcaccgtg
     1381 agcacgccgc acaccaccag cgtggggcgc acgccaaaga cgtgggcggc gctgcccacc
     1441 gtgaagctgc ccaggggcgc cacgcccagg aaggccatgg agaacagggc catcacccgg
     1501 ccgcggaagg cgttatccac ctgcgtctgg atcagcgtat tgctgccggc gatggtcagg
     1561 atgatgctgc cgcccagggc cagcagcacc ggcaccgcca tccccacgcc gggcgacagc
     1621 gcgaagagga tcaccgccac gcccaccgtg ggcgccgcca cggccacctt ctcggccagc
     1681 ccgccggtgt ccgcgcgccc cgccagataa aagctggcca ccagcgaacc cgcccccgcg
     1741 ctgcccacca gcaggccgaa gaggcgcgcg ccgccgccga actcctcctt ggcgaagagg
     1801 ggcatcagca ccacgtaggg cgccgcgcac aggctgatcg ccgccaccag ccgcagggac
     1861 gcgcggatct cccgatgggc catcgcgtag cgccagccct cccgcagggc ctgcaagggc
     1921 ggcggcgcgg gcgggcgatc ccccgcggca ggcaggcgga tcgcccgcag cgccatcaac
     1981 accgccagat aggacgccgc gttgagcaga aagcacaccg cctcgcccac ctccgccacc
     2041 acaaagcccg ccagcgccgg ccccacgaag cgcgccgtgt tcatcaggaa ggaattgagg
     2101 gcgatggcat tgggcagatc gtccggatcg tccaccagct gcaccgcgat cgcctgccgc
     2161 gccggcacat ccagcgcgtt gatgcagccc agcacaaagg ccagcccgac cagatgcccc
     2221 ggcgtcgccg catccttcca cgccagcgcc gccagcagca gcgcctgcac catggacaag
     2281 gcctgcgtcg ccagcaacac ccgccgccgg tccagccggt ccgtcaccac cccgccgaag
     2341 gccccgaaca acagaatcgg cgcctgactc gcaaacccca ccatccccaa cacaaacgcc
     2401 gaccccgaca
//
LOCUS       DAOQMM010000189         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_34791_length_2401_cov_40.968883, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000189 DAOQMM010000000
VERSION     DAOQMM010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34791_length_2401_cov_40.968883"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="asd"
                     /locus_tag="PLH85_16980"
     CDS             <1..399
                     /gene="asd"
                     /locus_tag="PLH85_16980"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586105.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNB66313.1"
                     /translation="LTRMIHVPGALFSVNPTTARGVPGLFARNERVVCVFENDRFGPF
                     VLVLVGATIVGSMATVWHGQVNPPRPGTVREWDYAPGQVELLKGAEMGRFLLGSTVVL
                     LFPKGRIAFTPTWQPGGAIRMGEAMGRSRL"
     gene            416..1588
                     /locus_tag="PLH85_16985"
     CDS             416..1588
                     /locus_tag="PLH85_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB66314.1"
                     /translation="MSAPRRVLPQIVVAQLFGTSLWFSANSAGDDLMRAWQLLPADLG
                     HLTNAVQLGFILGTLGFSLTGLADRYPASRIFALCALIGAAANAAFALFADSLGTALV
                     LRFGVGLALAGVYPLGMKLVVSWVPEQAGAALAWLVGMLTLGTALPHGVRMLDTGWGW
                     QATVLVSSGLALVAAGLIARLGDGPHLVRRHGAPPLRLGAVLMSFALPRFRASACGYF
                     GHQWELYAFWTLVPLLAGTLRPGPVAGPAFAVIGIGAVGCVLGGVLSRRIGSARVAAL
                     ALALSAACCAVYPWVARGPEALAAAVMLLWGASVVADSPHFSALSARACPPEIVGSAL
                     AFQNAIGFAITMGSIALTTRLWPVLHEAVAWVLLPGPLLGLAALYPLWRRGADRPA"
     gene            complement(1607..2074)
                     /locus_tag="PLH85_16990"
     CDS             complement(1607..2074)
                     /locus_tag="PLH85_16990"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB66315.1"
                     /translation="MDVKIDPKILRQCRDARAWSQERLAEAAGLSVRTVQRVEADGRA
                     SAETRQALAGALGMEVARLSPPQDARAEPYVPTVVSPVPAAFATAGERHRCGRRGRFS
                     RHLGIYVAMSLLFIVLDVQRDGRLDWAWWPILGWGIGVMNHFFATRRHSAKGA"
     gene            complement(2186..>2401)
                     /locus_tag="PLH85_16995"
     CDS             complement(2186..>2401)
                     /locus_tag="PLH85_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNB66316.1"
                     /translation="DDPGLSRAERREVQTLLIARGYPLGTVDGMLGDASRAAIRSEQQ
                     RLGLAPDGRAGQRFLAALRSAVPPTVR"
BASE COUNT          269 a          918 c          850 g          364 t
ORIGIN      
        1 ctgacgcgga tgatccacgt gcccggcgcg ctgttctcgg tcaatccgac caccgcccgc
       61 ggcgtgcccg gcctgtttgc ccgcaacgag cgtgtggtgt gcgtcttcga gaacgaccgc
      121 ttcgggccct tcgtgctggt gctggtgggc gccaccatcg tcggcagcat ggccaccgtg
      181 tggcacggcc aggtcaatcc gccgcggccc ggcacggtgc gggaatggga ctacgcgccg
      241 ggccaggtcg agctgctgaa gggcgccgag atggggcgct tcctgctcgg ctcgacggtc
      301 gtcctgctct tccccaaggg ccgcatcgcc ttcaccccca cctggcagcc gggcggtgcg
      361 atccgcatgg gcgaggcgat gggccgcagc cggctgtgat tccggccctc agaccatgag
      421 cgcgccgcgc cgcgtcctgc cgcagatcgt cgttgcccag ctcttcggca cctccctgtg
      481 gttctcggcc aacagcgccg gcgacgacct gatgcgcgcc tggcagctgc tgccggcgga
      541 cctgggccac ctcaccaacg ccgtgcagct cggcttcatc ctgggcaccc tgggcttctc
      601 cctcaccggg ctggcggacc gctatcccgc cagccgcatc ttcgccctgt gcgccctgat
      661 cggcgccgcc gccaacgccg cctttgccct ctttgccgac agcctgggca cggcactggt
      721 cctgcgcttc ggcgtgggcc tggcgctggc cggggtctac ccgctgggca tgaagctggt
      781 ggtgagctgg gtgccggagc aggccggcgc ggccctggcc tggctggtgg gcatgctcac
      841 cctgggcacc gccctgcccc acggcgtgcg catgctcgac acgggctggg gctggcaggc
      901 cacggtgctg gtttcgtcgg gcctggccct cgtcgccgcc ggcctgatcg cccgcctcgg
      961 cgacgggccg cacctggtgc gtcgtcacgg cgccccgccc ctgcgcctcg gcgccgtgct
     1021 gatgagcttc gccctacccc gcttccgcgc ctcggcctgc ggctacttcg ggcaccagtg
     1081 ggagctttac gccttctgga cgctggtgcc gctgctggcc ggcacgctgc ggccggggcc
     1141 ggtggcgggc cccgccttcg cggtgatcgg catcggcgcc gtcggctgcg tgctgggcgg
     1201 cgtcctcagc cggcgcatcg gcagcgcccg cgtggcggcc ctcgcgctgg ccctgtcggc
     1261 ggcctgctgt gcggtctatc cgtgggtggc ccgcggaccg gaggcgctgg ccgcggcggt
     1321 gatgctgctg tggggcgcca gcgtggtggc ggattcgccc cacttctcgg ccctctccgc
     1381 gcgcgcctgt ccgccggaga tcgtgggcag cgccctggcc ttccagaacg ccatcggctt
     1441 cgccatcacc atgggctcca tcgccctcac cacccgcctg tggccggtgc tccatgaagc
     1501 cgtcgcgtgg gtgctgctgc ccggcccgct gctgggcctg gccgccctgt accccttgtg
     1561 gcggcggggc gccgacagac cggcctgaac gccgctgcag gcgccgtcag gcccccttcg
     1621 cggagtgccg ccgggtcgca aaaaagtggt tcatcacgcc gattccccag cccaggatcg
     1681 gccaccacgc ccagtcgaga cgcccgtcgc gctgcacgtc cagcacgatg aacagcaacg
     1741 acatcgcgac gtagattccc agatggcggg aaaaacgacc ccgccgtccg cagcggtgcc
     1801 gctcaccggc cgtcgcgaat gccgccggga cgggcgacac cacagtgggc acgtagggct
     1861 cggcgcgtgc atcctgcggc ggcgagaggc gggccacctc catgcccagc gcccccgcca
     1921 gggcctgacg tgtctccgcc gaggcacgtc cgtccgcctc gacgcgctgc acggtccgca
     1981 ccgacaggcc ggcggcctcg gccagccgtt cctgggacca ggcccgcgcg tcacggcact
     2041 ggcgcaggat tttcgggtcg atcttcacat ccatgatccg gctgactcca cgcactgaag
     2101 ggtcgcccag cgtgccgggc gacggcccgt ctgaccacgc cagcgacccg acaaagaccc
     2161 gacagcgccc atccggacgg ccgtcctagc ggaccgtggg cggcaccgcg ctgcgcaggg
     2221 cggcgaggaa gcgctgcccg gcacgcccgt ccggcgccag gcccaggcgc tgctgctcgc
     2281 tgcggatggc ggcgcggctg gcgtcgccga gcatgccgtc cacggtgccg agggggtagc
     2341 cgcgggcgat gaggagggtc tgcacctcgc ggcgttcggc gcgggacagg ccggggtcgt
     2401 c
//
LOCUS       DAOQMM010000190         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_35401_length_2376_cov_41.141749, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000190 DAOQMM010000000
VERSION     DAOQMM010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35401_length_2376_cov_41.141749"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..340)
                     /locus_tag="PLH85_17000"
     CDS             complement(2..340)
                     /locus_tag="PLH85_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HNB66317.1"
                     /translation="MLELARIFGLFVLTALAEITGCYLPWLVWTRGHTPWLLLPAALS
                     LGLFAWLLTLHPGAAGRIYAAYGGVYVAVALLWLWQVDGVRPSRWDWIGSAVCLAGMA
                     IIALQPGREG"
     gene            complement(395..1306)
                     /locus_tag="PLH85_17005"
     CDS             complement(395..1306)
                     /locus_tag="PLH85_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB66318.1"
                     /translation="MYATFRQLQLFLAVAEHGSISAAAKACHVTQPTVSMQLRELADS
                     VGLPLYEVIGKRVFLTEAGQLVRQSALAIRDEWAALGTRVDALRGLQRGCLRVALVST
                     AKYFVPTLLGAFCRQYPDVEVSLSILNRDGVLERLQANADDLCIMSLPPDGDEVSREA
                     FLPNRLVVVAPAGHPLAAEQAIAPAALAGERFILREPGSGTRRACERHFATLGWQPTV
                     RMALGSNEAIKHSVAAGLGLAVLSRHTLHADPAVDGLAILDVAGFPIHANWYIVQPRG
                     KRLSPIAAEFLQHLREYVDSLPATTRA"
     gene            1399..>2376
                     /locus_tag="PLH85_17010"
     CDS             1399..>2376
                     /locus_tag="PLH85_17010"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNB66319.1"
                     /translation="MNSLLDPAILFFLFGVAAGLLKSNLEIPPAISRFLSLYLLMALG
                     LKGGFALAHSGLTAQVASSLLVAVSLAVIVPLVGFTVLRRKVGGFDAAAIAATYGSVS
                     AVTFITTTQYLESHGYPFGGHMAAALALMESPAIVLAVLLANHLRQSATAGVQVHADG
                     TATLGPAPGGVSFGKVLHESLTDGAQLLLLGAMVVGIVTGDAGKAAMQPFSGDLFKGM
                     LAFFLLDMGLLAARQLPQLRGASPWLPAYALLAPLVHASLALGLCLLAGIEAGNAALL
                     MVLAASASYIAVPAALRFAIPEARPSLYVGMSLGLTFPFNILLGIPLYVS"
BASE COUNT          359 a          908 c          729 g          380 t
ORIGIN      
        1 ctcagccctc gcgccccggc tgcagcgcga tgatcgccat gcccgccagg cacaccgcgc
       61 tgccgatcca gtcccagcgg ctcgggcgca ctccgtccac ctgccacagc cacagcagcg
      121 ccaccgccac atacacgccg ccgtaggctg cgtagatgcg tcccgcggcg ccggggtgga
      181 gggtcagcag ccacgcgaag aggccgaggg acagcgccgc cggcagcagc agccaggggg
      241 tgtgcccgcg cgtccacacc agccagggca ggtagcagcc ggtgatctcc gccagcgcgg
      301 tgaggacgaa cagcccgaag atgcgtgcca gttccagcat ggtgagctcc ctgaatccct
      361 gaaaagcctg tccgccgcgc tgcgccggcg atcctcacgc ccgcgtcgtc gccggcagac
      421 tgtccacata ttcccgcaga tgctgaagga actccgcggc gatgggcgac agacgcttgc
      481 cgcggggctg cacgatgtac cagttggcgt gaatcggaaa gcccgccacg tccaggatcg
      541 ccagcccgtc caccgccgga tcggcgtgca gcgtgtggcg cgacagcact gccagtccca
      601 gtcctgccgc caccgaatgc ttgatcgcct cgttgctgcc cagggccatg cgcaccgtgg
      661 gctgccagcc cagcgtcgca aaatgccgct cgcacgcgcg tcgcgtgccc gagcccggtt
      721 cccgcaggat gaagcgttcg cctgccaggg cggccggcgc aatcgcctgt tcggcggcca
      781 ggggatgtcc ggcgggggcc accaccacca gccggttggg caaaaaggct tcgcggctca
      841 cctcgtcccc gtccggcggc agcgacatga tgcacaggtc gtccgcgttg gcctgcaggc
      901 gctccagcac cccgtcccgg ttgaggatgg agaggctcac ctccacgtcc gggtactggc
      961 ggcagaaggc gcccagcagg gtcggcacaa agtacttcgc ggtgctcacc agggccaccc
     1021 gcaggcagcc ccgctgcaaa ccgcgcaggg catccacgcg ggtgcccagg gccgcccact
     1081 cgtcgcggat cgccagggcc gactggcgca ccagctgccc cgcctcggtg aggaagacgc
     1141 gcttgccgat cacctcgtag aggggcaggc cgacgctgtc ggccaactcg cgcagctgca
     1201 tggacaccgt cggctgggtc acatggcagg ccttggccgc cgcgctgatg ctgccgtgct
     1261 cggccaccgc aagaaagagc tgaagctgcc gaaacgtcgc gtacatagag ttttgtcgat
     1321 aaaaacaata tttattatct attttactcg atcattgctg cgccgtaaca tgaatccaga
     1381 tccttacgga gcctcatcat gaacagtctg ctcgatcccg caatcctctt cttcctcttc
     1441 ggggtggccg cgggcctcct caaatccaat ctggaaatcc cgcccgccat ctcccgcttc
     1501 ctgtccctgt atctcctcat ggcgctgggc ctcaagggcg gttttgcgct ggcccattcc
     1561 ggcctcaccg cccaggtggc gagcagcctg ctggtggcgg tgagcctggc cgtcatcgtg
     1621 cccctcgtcg gcttcaccgt gctgcgccgc aaggtgggcg gattcgacgc cgccgccatc
     1681 gccgccacct acggctcggt gagcgccgtc accttcatca ccaccaccca atacctggag
     1741 agccacggct accccttcgg cggacacatg gccgccgccc tggccctgat ggaatcgccc
     1801 gccatcgtcc ttgccgtgct cctcgccaac cacctgcgcc agagcgccac cgccggcgtg
     1861 caggtgcatg cggacggcac cgccaccctg ggccctgcgc cgggcggcgt gtctttcggc
     1921 aaggtgctgc acgaatccct caccgacggc gcgcaactgc tcctgctggg ggcgatggtc
     1981 gtcggcatcg tcaccggcga cgccggcaag gccgccatgc agcccttctc cggcgatctc
     2041 ttcaagggca tgctggcctt cttcctcctg gacatgggcc tcctcgccgc acgccagctg
     2101 ccccagctgc gcggcgcctc cccgtggctg ccggcttacg cgctgctcgc cccgctggtc
     2161 cacgccagcc tcgccctggg cctgtgcctg ctcgccggca tcgaggcggg caacgccgcc
     2221 ctgctgatgg tgctcgccgc cagcgcctcc tacatcgccg tgcccgccgc cctgcgcttc
     2281 gccatcccgg aagcccgccc gtccctgtac gtgggcatgt ccctgggcct caccttcccc
     2341 ttcaacatcc tgctggggat ccccctctac gtgagc
//
LOCUS       DAOQMM010000191         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_35692_length_2364_cov_45.226072, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000191 DAOQMM010000000
VERSION     DAOQMM010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35692_length_2364_cov_45.226072"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..2050
                     /gene="ftsH"
                     /locus_tag="PLH85_17015"
     CDS             236..2050
                     /gene="ftsH"
                     /locus_tag="PLH85_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNB66320.1"
                     /translation="MEKKTQFSLWYFLFALFAVFTLHDLWVQVRTTEPLPYSEFQRRL
                     KAGEVAEIAIADNTIQGSFKQALPDGRQKFVTTRVDPELARDLGQYDVKFTGVVESTL
                     LRDILGWVLPAVIFVAIWLFAMRRFGGKQGFGGGFMNLGKSKAKVYVEANTGVSFADV
                     AGVDEAKDELKEVVGFLKDPKSYGRLGGRVPKGVLLVGPPGTGKTLLAKAVAGEAGVP
                     FFSISGSEFVEMFVGVGAARVRDLFEQARAKAPAIIFIDELDAMGRARGAFAMGGHDE
                     KEQTLNQLLVELDGFDSSSGLVLLAATNRPEVLDAALLRAGRFDRQVLVDRPDKKGRI
                     AILQVHMKKITLAPDLDAETVAALTPGFSGAELANLCNEAALVATRRNGSAVTVDDFT
                     VAVERIVAGLEKKNRVLNEHERTVVAHHEMGHALVAMALPGTDPVHKISIIPRGVGAL
                     GYTIQRPTEDRFLMTRAELENKMAVLLGGRAAEQIIFGEVSTGAADDLQKVTSIARSI
                     VMRFGMDDQLGNVAYDTERNTFLGQAAAAPAERSYSEETAREIDCAVRHIVARAFERS
                     TAILSAHKDLLERSAADLLQRETFDEADLKALHTRVAA"
BASE COUNT          386 a          915 c          712 g          351 t
ORIGIN      
        1 cagtggctgt ccctgccgat gatcgtgatc ggcatcgtca tggcggtgat tgccgtccgc
       61 cgggcggccg acacctgatc gccccacggc ccggccgcgc ccgcggccgg actgcccctc
      121 cacgtgccgc acacacccgc ccccacggcg aacaccggga ggcgttcgcc ccggacgctt
      181 gaatcctgcg gcagacgccc ccatctgccg aaaacctccc ttgtttccgc ccgccatgga
      241 aaagaaaacc cagttcagcc tgtggtactt cctcttcgct ctgttcgcag tgttcaccct
      301 gcacgacctg tgggtccagg tacgcaccac cgagccgctg ccctacagtg aattccagcg
      361 caggctgaag gccggcgagg tggccgagat cgccatcgcc gacaacacca tccagggcag
      421 cttcaagcag gccctgcccg atggccgcca gaagttcgtc accacccgcg tcgatcctga
      481 actggccagg gatctgggcc agtacgacgt caaattcacc ggcgtcgtgg agagcaccct
      541 gctgcgggac atcctcggct gggtgctgcc cgcggtcatc ttcgtcgcca tctggctctt
      601 tgccatgcgc cgcttcggcg gcaagcaggg cttcggcggc ggcttcatga acctgggcaa
      661 gagcaaagcc aaggtctacg tggaggccaa taccggcgtg agctttgccg acgtggccgg
      721 cgtggacgag gccaaggacg agctcaagga agtcgtcggc ttcctcaagg accccaaatc
      781 ctacggccgc ctgggcggac gcgtacccaa gggcgtgctg ctggtcggcc cgccgggcac
      841 cggcaagacc ctgctcgcca aggccgtcgc cggcgaggcg ggcgtgccct tcttctcgat
      901 ctccggctcc gagttcgtcg agatgttcgt cggcgtgggg gccgcccgcg tccgcgacct
      961 cttcgagcag gcccgcgcca aggcgcccgc catcatcttc atcgacgaac tggacgccat
     1021 gggccgcgcc cgcggtgcct tcgccatggg cggccacgac gagaaggaac agaccctcaa
     1081 ccagctgctt gtcgaactgg atggcttcga ctcctcctcc ggcctggtcc tgctggccgc
     1141 caccaaccgg ccggaagtcc tcgacgccgc cctgctgcgg gccggccgct tcgaccgtca
     1201 ggtgctggtg gaccgcccgg acaagaaggg ccgcatcgcc atcctgcagg tgcacatgaa
     1261 gaagatcacc ctggcccccg acctggacgc ggaaaccgtc gccgccctga cacccggctt
     1321 ctccggcgcc gagctggcca acctgtgcaa cgaggcggcc cttgtcgcca cccgccgtaa
     1381 cggcagcgcc gtcaccgtcg acgacttcac cgtggcggtg gagcgcatcg tcgccggcct
     1441 cgagaagaag aaccgtgtcc tcaacgaaca cgagcgcacc gtcgtcgccc accacgagat
     1501 gggccacgcc ctggtcgcca tggccctgcc cggcaccgac ccggtgcaca agatcagcat
     1561 catcccgcgg ggcgtcggcg ccctgggcta caccatccag cgtcccaccg aagaccgctt
     1621 cctgatgacc cgcgccgaac tggagaacaa gatggccgtg ctgctgggcg ggcgcgccgc
     1681 agaacagatc atcttcggcg aagtctccac gggcgctgcc gacgacctgc agaaggtcac
     1741 ctccatcgcc cgctccatcg tcatgcgctt cggcatggac gaccagctcg gcaacgtggc
     1801 ctacgacacc gagcgcaaca ccttcctcgg tcaggcggcg gccgcgccgg ccgaacgcag
     1861 ctattcggag gaaaccgccc gcgagatcga ctgcgcggtc cgccacatcg tcgcccgcgc
     1921 cttcgagcgc agcacggcga ttctcagcgc ccacaaggat ctgctggagc gcagcgccgc
     1981 cgacctgctc cagcgggaaa ccttcgacga agccgacctc aaggccctcc acacccgcgt
     2041 cgccgcctga cgccaccccg accgcgccgc gcccgcctca gcgcgggcgt ccgcgtcgcg
     2101 gtccctgcac tgctcgcccg cgcggcgatg gcagggctac aatcgtcctt gctgttccat
     2161 ccagcccgaa gaaccgtgtg ccgcctcgct ccggcgcccc tgcaggcgga cgcaccccga
     2221 gttcggacaa ccacctttgc aaggagagtc ctcccatgtt ccgcctccgc ctgcctctcg
     2281 tcactctggc ctccgccgct ggcctgctcg ccgccctggc ccagcccgcc gccgcccagg
     2341 aagtgcccac cctcgccaag atca
//
LOCUS       DAOQMM010000192         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_35795_length_2359_cov_36.869358, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000192 DAOQMM010000000
VERSION     DAOQMM010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35795_length_2359_cov_36.869358"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..467)
                     /locus_tag="PLH85_17020"
     CDS             complement(75..467)
                     /locus_tag="PLH85_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB66321.1"
                     /translation="MFSHIFVGTNDFERALAFYRPLMDVLGIQARFCDPARPWAGWQS
                     APDPRPLFLLGRPWDGQPHTPGNGQMVAFLAPSRAAVADAHRLALAHGGRDEGAPGLR
                     PEYHAHYFGAYFRDPDGNKVCVVCHAPV"
     gene            complement(624..1181)
                     /locus_tag="PLH85_17025"
     CDS             complement(624..1181)
                     /locus_tag="PLH85_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB66322.1"
                     /translation="MPAAIVPIPSFVPKVMTVPTVTPLSELHADIDRRVATLRSEHAD
                     WPCAKGCDTCCRRLADVPRLTASEWGLLREGLAALPAPRRAGFRAAVARLADTPERPL
                     TCPFLDPVSGACSVYAYRPVACRTYGFYADRDGGLYCHDIEARVDDGRLAGVVWGNHA
                     AVLTRLARTGETRPLTEWYADWHET"
     gene            1549..>2359
                     /locus_tag="PLH85_17030"
     CDS             1549..>2359
                     /locus_tag="PLH85_17030"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HNB66323.1"
                     /translation="MLVLCLAYAVTGWLALQIAVPPGYAAPLFPPAGIALAAVMLYGP
                     SCLPAIFLGSLLVQVPAAIQTGIDIQRWPGLLVPAFGAMLQARVGYGLARRLIGLPNT
                     LDSQDSILNFVALVAPLGCVVSASIAVPTLVLMHAIPPSESTFTWINWWAGDTLGVLV
                     MVPLTLVLFGLPAADWRPRRLTVAVPMSVALTLLAVTFMQVREWETQRLQTQFNRDAE
                     FVASLVQKRFDAQLDQLQALERLTAVHPGLSADEWRDFVTPMMARNPGTQNF"
BASE COUNT          354 a          871 c          806 g          328 t
ORIGIN      
        1 cgagcagcga cccgccgcca acacgaccgc tgcgcgcctg tgcgcgccgc ggcggcgccc
       61 agccagacgg cgccttatac cggggcgtgg cacaccacgc acaccttgtt cccgtccgga
      121 tcgcggaaat acgccccgaa gtagtgtgcg tggtactccg ggcgcaggcc gggcgcaccc
      181 tcgtcgcggc ccccgtgcgc cagggcgagg cggtgggcgt ccgccacggc ggcgcggctt
      241 ggcgccagga aggccaccat ctgcccgttg ccgggcgtgt ggggctgacc gtcccagggg
      301 cggcccagga ggaacagggg acgtggatcc ggcgccgact gccagccggc ccacggccgc
      361 gccggatcgc agaaacgggc ctggatgccg aggacgtcca tcaaggggcg ataaaaggcg
      421 agggcgcgtt cgaaatcatt ggtgccgacg aagatgtggg aaaacatggc tgctccagga
      481 aacaggggaa gatccggacc cgccggaccc gatccgctcg acaccgcgac accccgcggg
      541 ggcggatgag cgcccccccg cggacgggcc gacggcgccg ggcatcgggc ggcgcggtgc
      601 gacgggcgga gcgcccgcgc ggttcaggtt tcatgccagt ccgcgtacca ctcggtcagg
      661 ggccgcgtct cgccggtcct ggcgaggcgg gtgagcaccg ccgcatggtt gccccagacc
      721 accccggcca gacgcccgtc gtccacccgg gcctcgatgt catggcagta cagcccgccg
      781 tcccggtcgg catagaagcc gtaggtgcgg caggccaccg ggcgataggc ataaaccgag
      841 caggcaccac tcaccgggtc gaggaagggg caggtcaggg ggcgctccgg cgtatcggcc
      901 aggcgcgcca ctgccgcgcg aaagccggcc cgccggggcg ctgggagggc ggccaggcct
      961 tcccgcagca gcccccattc ggacgcggta agacggggca catcggcgag ccgccggcag
     1021 caggtgtcgc agcccttggc acagggccag tctgcatgct cactgcgcag ggtggcgaca
     1081 cgccgatcga tgtcggcatg gagttcggac aggggggtga cggtgggcac ggtcatgacc
     1141 ttcggcacga aggaggggat cggtacgata gcagccggca tccgcggcgg gaatccgccc
     1201 cgacgggacc gccgatgcgc cgattccgga cggacgcgcg ccgcgacacg cgccctcagc
     1261 cggatcggac cgcaacccac gccggcgccg gacccggatc ggccgcctct ccggcccggc
     1321 ccatcgagac gggtgagacg taccggatcg cccctcaccg cgccctgcac ccttgtccgc
     1381 cgggcgcccc aggtccggca cgcatcgatc catcgccctg cacgtcactc cgacgccaat
     1441 cggtcgtaaa ataaggttaa atgacaggct gtcgtcccct gcatcgctcc acgtgaaccg
     1501 tctgcccacc tgatgcattt ctccccgcgg catcgcacgc tgcttcgcat gctggtcctg
     1561 tgcctcgcct acgccgtcac cggctggctt gcgctgcaga tcgccgtccc ccccggttac
     1621 gcggcaccgc tcttcccccc cgccggcatc gcgctggccg cggtcatgct ctacgggccg
     1681 tcctgcctgc cggcgatctt tctcggatcg ctgctggtcc aggtgccggc cgccatacag
     1741 accggcatcg acatccagcg ctggccgggg ctgctggtgc cggccttcgg cgcgatgctg
     1801 caggcccgcg tcggctatgg cctggcacgg cgcctgatcg gactgcccaa caccctggac
     1861 agtcaggaca gcatcctgaa tttcgtcgcc ctggtggccc cgctgggctg tgtcgtcagt
     1921 gccagcatcg cggtgccgac gctcgtgctg atgcacgcca tcccgccctc cgagagcacc
     1981 ttcacgtgga tcaactggtg ggccggcgac accctcggcg tgctggtgat ggtgcccctg
     2041 accctggtcc tgttcggcct gcccgctgcg gactggcgcc cccggcgcct caccgtcgcc
     2101 gtccccatga gcgtcgccct gacgctcctg gcggtcacct tcatgcaggt gcgggaatgg
     2161 gaaacccagc gcctgcagac ccagttcaac cgggatgccg aattcgtcgc cagcctggtg
     2221 cagaagcgct tcgacgccca gctcgaccag ttgcaggcgc tggagcgcct caccgcggtg
     2281 caccccggtc tcagcgccga cgaatggcgc gatttcgtca cgccgatgat ggcccgcaat
     2341 ccgggcaccc agaacttcg
//
LOCUS       DAOQMM010000193         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_36255_length_2341_cov_66.150919, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000193 DAOQMM010000000
VERSION     DAOQMM010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36255_length_2341_cov_66.150919"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /gene="mobH"
                     /locus_tag="PLH85_17035"
     CDS             <1..1628
                     /gene="mobH"
                     /locus_tag="PLH85_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HNB66324.1"
                     /translation="VQQFPASENHHHAYPGGMLDHGLEIVAYALKLRQSYLLPAGVTP
                     EAQAAQAEAWTAGTAYAALLHDIGKIAVDLHVEHADGSVWHPWHGPLRKPYRFRYRKE
                     REYRLHSAATGLLYARLLDRDIFDWLSGYPDLWAALLYVLAGQYEHAGTLGELVVQAD
                     QASVAQELGGDPSKALAAPKHALQRKLLDGLRYLLKEEFKLNQAGPADGWLTQDALWL
                     VSKTVSDKLRAHLLSQGIDGIPASNTAVFNVLQDHGIVQPTPDGKAIWKATVTSDAGW
                     SHAFTFLKLSPAMIWDAADRPAPFAGRVQVEEEQAEPTPQATTVADGPRAEGIEAASA
                     STAPIASAAMDTGVAALLDLLGDTAPPTAPEIPSSPVAEPEPAPSTVPPPQMGLAPSQ
                     DRAEPSGAHFMAWLRQSIQTRKLIINDAKALVHTVAGTTYLVSPGVFQRYAQEYLQVA
                     ALAKQEKLEGWQWVQKRFEKLGQHRKQPSGLNIWTCEVTGPRKSRRLHGYLLASPDAL
                     FQETPPDNPYLRLLNEAAKREDSALGGKDDDQA"
     gene            complement(1655..1954)
                     /locus_tag="PLH85_17040"
     CDS             complement(1655..1954)
                     /locus_tag="PLH85_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB66325.1"
                     /translation="MPKRSIQRGRPTGTTTYEAEPAIAFGAAVREERTNQGIAQETLA
                     HMAGIERSHMGKIERGEHVPTLPLILKIARALKCSSAHLMTLTEAKLAESAPSAD"
     gene            2060..>2341
                     /locus_tag="PLH85_17045"
     CDS             2060..>2341
                     /locus_tag="PLH85_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB66326.1"
                     /translation="MESSDITAEIRMAVLGEWVPPQLADVLALQRAEEPETHAVLAGW
                     TDPGRGAELPDDGFDFALSAVARQWPGWVCEPLWHDTLAVAVAKRSHLLA"
BASE COUNT          403 a          796 c          771 g          371 t
ORIGIN      
        1 tggtccagca gttcccggcc tccgagaacc accaccacgc ctatccgggc ggcatgctgg
       61 accacggcct ggagatcgtc gcctatgcgc tgaagctgcg gcagtcatac ctgctgcctg
      121 cgggcgtcac gccggaggca caggcggccc aggccgaagc ctggaccgca ggcacggcct
      181 acgcggccct gctgcacgac atcggcaaga ttgcggtcga tctgcacgtc gagcatgccg
      241 acggcagcgt ctggcatccc tggcacggcc cgctgcgaaa gccctaccgc ttccgttacc
      301 gaaaggagcg cgagtaccgc ctgcacagcg ccgccaccgg gctgctctac gcgcgccttc
      361 tcgatcggga catcttcgac tggctcagcg gctatcccga cctctgggcc gcgctgctgt
      421 acgtgctggc cggccagtac gagcacgccg gcacgctcgg cgagctggtc gtgcaggccg
      481 accaggcatc ggtcgcccag gaactcggcg gcgatcccag caaggcgctg gcggcgccca
      541 agcatgcgct gcaacgcaaa ttgctcgacg gcttgcgcta cctgctcaag gaagaattca
      601 agttgaacca ggccggcccg gcggacggtt ggctgaccca ggacgccttg tggctggtga
      661 gcaagaccgt ctccgacaag ctgcgcgcac atctgctgtc gcagggcatc gacggcatcc
      721 cggcgagcaa tacggcggtg ttcaacgtgc tgcaggatca cggcatcgtg caaccgacgc
      781 cggatggcaa ggcgatctgg aaggctaccg tcaccagcga cgccggctgg tcgcacgcct
      841 tcaccttcct gaaactctcg ccggcgatga tctgggatgc cgccgaccgg ccggcgccgt
      901 tcgcaggccg tgtgcaggtc gaagaggaac aggcggagcc gacgccgcag gcgacaacgg
      961 tggccgatgg gccgcgcgcg gaaggcatcg aagctgcatc cgcgagcacg gcgccgatcg
     1021 catccgcagc catggacacc ggcgtggccg cgctgctcga cctgctgggc gacaccgctc
     1081 cacccacggc accggagatc ccgagttccc ctgttgccga gcccgagccg gccccttcga
     1141 ccgtgccccc gccgcagatg ggcctcgcgc cttcccagga tcgcgcggag ccctccgggg
     1201 cgcacttcat ggcctggctg cgtcagagca tccagacgcg caagctcatc atcaacgacg
     1261 ccaaggccct ggtgcatacc gtcgccggta cgacctacct cgtcagcccc ggcgtgttcc
     1321 agcgctacgc gcaggagtac cttcaagtcg ccgcgctggc caagcaggag aagctggagg
     1381 ggtggcagtg ggtacagaag cgcttcgaga agctgggaca gcaccgcaag cagccgagcg
     1441 ggctgaacat ctggacctgc gaagtcacgg ggccgcgcaa gtcgcgccgg ctgcacggct
     1501 acctgctcgc cagcccggat gcgctgttcc aggagacgcc gccagacaac ccatacctgc
     1561 ggctcctcaa cgaggccgca aagcgcgaag attcagccct tgggggcaag gacgacgatc
     1621 aggcgtaggc atgcgaccgc tgtgcgggcc ggcttcaatc cgcggatggg gccgactctg
     1681 ccagcttggc ttcggtcaga gtcatcaagt gggcggaact gcatttcagc gcacgggcaa
     1741 tcttgaggat gagagggagc gtggggacat gctcgccgcg ctcgatcttg cccatgtgcg
     1801 accgttcgat gccggccatg tgggccagcg tttcctgagc gatgccctgg ttggttcttt
     1861 cttcccgcac ggcagcccca aacgcgatgg ctggttccgc ttcgtaggta gtggtgccgg
     1921 tggggcggcc cctttggatc gaacgctttg gcattgccaa gagcgtcgaa cacggtcacg
     1981 atttaaacca cgttaaactt aactcattca acggcttcga gatcagttgc cacgagttca
     2041 ccgcctgggg gagctggacg tggaatcgag tgacatcacc gccgaaattc gcatggccgt
     2101 cctcggcgaa tgggtgcctc cccagctcgc cgacgtgctc gccctgcagc gtgccgaaga
     2161 gccggagact catgccgtcc tggccggctg gacagatccc ggtcgaggcg cggagctgcc
     2221 ggacgacggc ttcgacttcg ccttgtctgc ggttgcccgg cagtggcctg gctgggtatg
     2281 cgagccgctg tggcatgaca cgctggcggt cgccgtggcc aagcgctccc acctgctggc
     2341 c
//
LOCUS       DAOQMM010000194         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_37509_length_2297_cov_50.810437, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000194 DAOQMM010000000
VERSION     DAOQMM010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37509_length_2297_cov_50.810437"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLH85_17050"
     CDS             <1..918
                     /locus_tag="PLH85_17050"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB66327.1"
                     /translation="VSELIVMAMNALLNAGDEVLVPAPDYPLWTAAVSLSGGTPRHYL
                     CDEANEWLPDLDDIRAKITPRTRALVIINPNNPTGALYPEETLRGIVDIARQHGLILY
                     ADEVYDKVLYDGAQHTSIASLSEDVLTITFNGLSKNYRSCGYRAGWLVVSGDKRAAAD
                     YIEGLNMLASMRLCANVPGQYAIQTALGGYQSIDDLVAPGGRMRRQRDLAHELISAIP
                     GVSCVKPKATLYMFPRLDPAVYPIADDQQFIAQLLEQQRVLLVQGTGFNWPHPDHFRL
                     VFLPHEDDLRDAIGRIARFLDGYRKRSGT"
     gene            986..2293
                     /locus_tag="PLH85_17055"
     CDS             986..2293
                     /locus_tag="PLH85_17055"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNB66328.1"
                     /translation="MKPIQVGLLGIGTVGGGTFTVLKRNEEEITRRAGRPIRITTVAD
                     KNLELARAVTGGDVKLTDDAFAVVSDPEIDIVVELIGGYGVAKELVLKAIENGKHVVT
                     ANKALLAVHGNEIFAAAQKKGVMVAFEAAVAGGIPIIKALREGLSANRIQWIAGIING
                     TTNFILSEMRDKGLPFADVLKEAQRLGYAEADPTFDIEGVDAAHKATLMASIAFGVPV
                     QFDKAYVEGISKLDATDIKYAEQLGYRIKLLGIARRRTQGIELRVHPTLIPEKRLIAN
                     VEGAMNAVLVHGDAVGATLYYGKGAGAEPTASAVIADLVDVTRLHTADPEHRVPHLAF
                     QPDQITDVPVLPIDDVETSYYLRLRVVDKPGVLADVTRILADQDISIDAMLQREPEEG
                     ESQTDIIILTHLTVEKNADTAIARIEALDSVNGKVTRLRLENL"
BASE COUNT          417 a          885 c          664 g          331 t
ORIGIN      
        1 gtgtccgagc tcatcgtgat ggccatgaac gcgctcctca acgctggtga cgaggtgctg
       61 gtgccggcgc ccgactatcc cctgtggacc gccgccgtga gcctctccgg cggcaccccg
      121 cgccattacc tgtgcgacga ggccaacgag tggctgcccg atctggacga catccgcgcc
      181 aagatcacgc cgcgcacccg cgccctcgtc atcatcaacc ccaacaaccc gaccggcgcc
      241 ctgtaccccg aagagaccct gcgcggcatc gtggacatcg cccgccagca cggcctgatc
      301 ctctacgccg acgaggtcta cgacaaggtg ctgtacgacg gcgcccagca cacctccatc
      361 gcctccctgt cggaggacgt gctcaccatc accttcaacg gcctgtccaa gaactaccgc
      421 tcctgcggct atcgcgccgg ctggctggtg gtgtccggtg acaagcgcgc cgccgccgac
      481 tacatcgaag gcctcaacat gctggcctcc atgcgcctgt gcgccaacgt gcccggccag
      541 tatgcgatcc agaccgccct cggcggctac cagagcatcg acgacctggt ggcgcccggc
      601 ggccgcatgc gccgtcagcg cgacctggcc cacgagctga tcagcgccat tcccggcgtc
      661 agctgcgtca agcccaaggc caccctgtac atgttcccgc gcctcgaccc ggcggtgtac
      721 cccatcgccg acgaccagca gttcatcgcc cagctgctgg agcagcagcg cgtgctgctg
      781 gtccagggca ccggcttcaa ctggccccac ccggaccact tccggctggt cttcctgccc
      841 cacgaggacg acctgcgcga cgccatcggg cgcatcgccc gcttcctcga cggctaccgc
      901 aagcgttccg gcacctgatc cccgacgggg cgccggttgt ggcgccccgc gttcatccac
      961 gttcactcac acgacccact cgaccatgaa acccatccaa gttggcctcc tgggcatcgg
     1021 caccgtcggc ggtggcacct tcaccgtcct caagcgcaac gaagaagaaa tcacccgccg
     1081 cgccggccgg ccgatccgga tcaccaccgt ggccgacaag aacctggagc tggcccgcgc
     1141 cgtcaccggc ggcgacgtca agctcaccga cgacgccttc gccgtggtca gcgaccccga
     1201 aatcgacatc gtcgtcgaac tgatcggtgg ctacggcgtg gccaaggagc tggtgctcaa
     1261 ggccatcgag aacggcaagc acgtggtcac cgccaacaag gcgctgctcg ccgtgcacgg
     1321 caacgagatc ttcgccgccg cccagaagaa gggcgtgatg gtggccttcg aggccgccgt
     1381 ggccgggggc atccccatca tcaaggccct gcgcgaaggc ctctccgcca accgcatcca
     1441 gtggatcgcc ggcatcatca acggcaccac caacttcatc ctctccgaga tgcgcgacaa
     1501 gggcctgccc ttcgccgacg tgctcaagga agcccagcgc ctgggctacg ccgaagccga
     1561 ccccaccttc gacatcgaag gcgtggacgc cgctcacaag gcgaccctga tggcctccat
     1621 cgcctttggc gtgccggtgc agttcgacaa ggcctacgtg gaaggcatca gcaagctcga
     1681 cgccaccgac atcaagtacg ccgagcagct gggctaccgc atcaagctgc tgggcatcgc
     1741 ccgccgccgc acccagggca tcgagctgcg cgtgcatccc acgctgatcc cggaaaagcg
     1801 cctcatcgcc aacgtggaag gcgccatgaa cgccgtgctg gtccacggtg acgccgtcgg
     1861 cgccacgctg tactacggca agggcgccgg tgccgagccc accgccagcg cagtcatcgc
     1921 cgatctcgtg gacgtcaccc gcttgcacac cgccgatccg gaacaccgtg tgccgcacct
     1981 ggccttccag ccggaccaga tcaccgacgt gccggtgctg cccattgacg acgtggagac
     2041 cagctactac ctgcgcctgc gcgtggtgga caagcccggc gtgctcgccg acgtcacccg
     2101 catcctcgcc gaccaggaca tctccatcga cgccatgctc cagcgcgagc cggaagaggg
     2161 cgagtcccag accgacatca tcatcctcac ccacctcacc gtcgaaaaga acgccgacac
     2221 cgccatcgcc cgcatcgagg cgctggattc ggtgaacggc aaggtgaccc ggctgcgtct
     2281 ggaaaacctc tgatccg
//
LOCUS       DAOQMM010000195         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_39422_length_2234_cov_49.425883, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000195 DAOQMM010000000
VERSION     DAOQMM010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39422_length_2234_cov_49.425883"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLH85_17060"
     CDS             <1..1011
                     /locus_tag="PLH85_17060"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNB66329.1"
                     /translation="LVGGQDELVFDGGSFALDRQGVLTWQGPVFAEDLTLLTFAGGAW
                     EEQTIPAPRPLEADVYDALVLGVRDYLGKNGFPGAIIGLSGGVDSALTLAVAVDALGA
                     DRVRAVMMPSPYTAQMSLDDSREMVRRLGVRYDEIPIEPAMRTFAEMLAPQFVGLPED
                     TTEENLQSRIRGMLLMALSNKTGAIVLTTGNKSEMATGYATLYGDMAGGFAVIKDLYK
                     TFVYRLCAWRNALHGQPVIPDNILTRPPSAELKPDQCDQDSLPPYEVLDAIIAAYMEE
                     DLSPAEIVARGYPEHDVRRTVAMLKRNEYKRRQAPPGVRVTQRGFGKDWRYPITSRYR
                     DA"
     gene            1081..1419
                     /locus_tag="PLH85_17065"
     CDS             1081..1419
                     /locus_tag="PLH85_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNB66330.1"
                     /translation="MKKIEAVIKPFKLDEVREALSEVGITGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLEVIVSSDVAEQAIEAIIKAARTGKIGDGKIFVMPVEQVVRI
                     RTGETNESAI"
     gene            1627..>2234
                     /gene="gltX"
                     /locus_tag="PLH85_17070"
     CDS             1627..>2234
                     /gene="gltX"
                     /locus_tag="PLH85_17070"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNB66331.1"
                     /translation="MASTVRTRFAPSPTGYLHIGGARTALFSWAFARRHGGTFVLRIE
                     DTDVARSTPEAVQAILDGMSWLDLNWDEGPFYQMQRMDRYKAVIQAMLAAGTAYPCYM
                     PPEELDALREAQRARGEKPRYDGRWRPEPGKELPAPPVGVQPVIRFKNPTEGVVAWDD
                     LVKGRIEIANAEMDDLIIARADGTPTYNFCVVVDDWDMGITHV"
BASE COUNT          375 a          789 c          722 g          348 t
ORIGIN      
        1 ctggtgggcg ggcaggacga gctggtcttc gacggcggct ccttcgccct cgaccggcag
       61 ggcgtgctga cctggcaggg accggtgttt gccgaggacc tgactctgct gaccttcgcc
      121 ggcggcgcct gggaggagca gacgatcccc gccccgcgcc cgctggaggc cgacgtgtac
      181 gacgccctgg tgttgggtgt gcgcgactac ctgggcaaga acggctttcc cggcgcgatc
      241 atcggcctgt ccggcggcgt ggattcggcc ttgaccctgg ccgtcgcggt ggacgccctg
      301 ggcgccgacc gggtgcgcgc ggtgatgatg ccctcgccct atacggcgca gatgagcctg
      361 gacgattccc gcgagatggt gcggcgcctg ggcgtgcgtt acgacgagat ccccatcgag
      421 ccggccatgc gcaccttcgc cgagatgctg gcgccccagt tcgtcggtct gcccgaggac
      481 accaccgagg agaacctcca gtcgcgcatc cgcggcatgc tgctgatggc cctgtccaac
      541 aagaccggcg ccatcgtcct caccaccggc aacaagagcg agatggccac cggctacgcc
      601 accctgtacg gcgacatggc gggcggcttc gccgtgatca aggatctcta caagaccttc
      661 gtctatcgtc tgtgcgcatg gcgcaacgcg ctgcacggcc agccggtgat cccggacaac
      721 atcctcaccc ggccaccctc tgccgaactc aagcccgacc agtgcgatca ggactccctg
      781 ccgccctacg aggtgcttga cgccatcatc gcggcctaca tggaagagga tctgtcgccg
      841 gccgagatcg tcgcccgcgg ctatccggaa cacgacgtgc gccgcaccgt ggccatgctc
      901 aagcgcaacg aatacaaacg gcgccaggcg ccgccgggtg tgcgcgtgac ccagcgcggc
      961 ttcggcaagg actggcgtta ccccatcacc tcccggtacc gggacgcgta aaacctgccc
     1021 ggccccttgg cgggccggca aatgcggcta aaatgccgaa ttgttttcag gagactgccc
     1081 atgaagaaaa tcgaagccgt gatcaagccc ttcaagctgg acgaagtccg ggaagccctg
     1141 tccgaagtgg gcatcaccgg cctgaccgtc accgaggtga agggtttcgg tcgccagaag
     1201 ggccacacgg agctgtaccg cggggccgaa tacgtggtgg atttcctgcc caagatcaag
     1261 ctggaagtga tcgtctcctc cgacgtggcc gagcaggcca tcgaggccat catcaaggcc
     1321 gcccgcaccg gcaagatcgg tgacggcaag atcttcgtga tgcccgtcga acaggtcgtg
     1381 cgcatccgta ccggcgagac caacgaatcg gcgatctgag cgtcgcctgc ggtcgccccg
     1441 ccgatgtgcg gggcccgcaa cgggtcttcc ccgatccccg ccccgcggcc gtccgcgcgg
     1501 cggggattgt cgttcacgag ccgtcccgtc ccgctggcct gcgggtgagt cggcgcgcca
     1561 ggccgatttt gcgatcctgg cgcagcgcgt agaatgccgc cttttcagca tttccggatc
     1621 tgtaccatgg cttccaccgt ccgcacccgc ttcgccccca gccccactgg ctacctgcac
     1681 atcggaggcg cgcgcacggc cctgttctcg tgggcctttg cccgccgtca tggcggcacc
     1741 ttcgtgctgc gcatcgagga caccgacgtg gcccgctcca cgcccgaggc ggtgcaggcc
     1801 atcctcgacg gcatgagctg gctggatctg aactgggacg agggcccctt ctaccagatg
     1861 cagcgcatgg accgctacaa ggcggtgatc caggcaatgc tcgccgccgg cacggcgtac
     1921 ccctgctaca tgccgccgga agaactggac gccctgcgcg aagcccagcg cgcccgcggc
     1981 gaaaagcccc gctacgacgg ccgctggcgg cccgagcccg gcaaggaact gcccgcgccg
     2041 cccgtcggcg tgcagccggt gatccgcttt aaaaacccga ccgagggcgt ggtggcgtgg
     2101 gacgacctgg tcaagggccg catcgagatc gccaacgccg agatggacga cctcatcatc
     2161 gcccgcgccg acggcacgcc cacctacaac ttctgcgtgg tggtggacga ctgggacatg
     2221 ggcatcaccc acgt
//
LOCUS       DAOQMM010000196         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_39786_length_2224_cov_48.489166, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000196 DAOQMM010000000
VERSION     DAOQMM010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39786_length_2224_cov_48.489166"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLH85_17075"
     CDS             complement(<1..334)
                     /locus_tag="PLH85_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66332.1"
                     /translation="MNTYFAALAVAMAVLTGFAGEAVAAPQAHRAAAPSKTAPKPVAR
                     VTPRPAPVKLAPKPVSRPAAQAQVRGKTAPARTGVRPAPAPVRAQARPAPVVKAPARA
                     PAPVASARG"
     gene            537..>2224
                     /locus_tag="PLH85_17080"
     CDS             537..>2224
                     /locus_tag="PLH85_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNB66333.1"
                     /translation="MLLPIEQRIAAELGVQPRQIESAVQLLDEGATVPFIARYRKEVT
                     GGLDDTQLRTLEERLGYLRELEDRRAAVLASIDEQGKLTPALREAVEHADTKQRLEDL
                     YLPYKQKRRTKAQIAREAGIEPLALALLADPGLSPDAEAAAYLNTDAGFADTRAVLDG
                     ARQILMERFAEDATLIGTLRSYLGEHGVVVSSVVAEKEADPAAAKFRDWFDFREPLAT
                     LPSHRALALFRGRNEGVLRLQLGLDSDPTDGSTPGPNPCEARIAGHVGVRDQGRPADA
                     WLRETVRWAWSVKISLHLELELLGQVRERAEEEAIRVFGRNLKDLLLAAPAGARATLG
                     LDPGLRTGVKVAVVDQTGKLLDTATIYPHEPRRDWDGSLHTLSKLCEKHGVSLIAIGN
                     GTASRETDRLAADLIKLRPELHMTKIVVSEAGASVYSASELAAREFPDLDVSLRGAVS
                     IARRLQDPLAELVKIDPKSIGVGQYQHDVNQTRLARALDSVVEDCVNAVGVDVNMASA
                     ALLARISGLNTTLAANIVAHRDAHGPFPSRAALLDVPRLGPKTFEQAAGFLRIP"
BASE COUNT          344 a          852 c          725 g          303 t
ORIGIN      
        1 gtccacgggc gctcgccacg ggggccggcg cacgggccgg cgccttgacc accggcgcag
       61 gccgagcctg ggcacgaacc ggcgcgggcg ccggacggac ccccgtacgg gcgggtgcgg
      121 tcttgccacg gacctgggcc tgcgccgccg gacgggacac cggtttgggc gcaagcttca
      181 ccggggcggg acggggcgtc acccgggcga cgggcttggg ggccgtcttg gacggcgcgg
      241 ctgcacgatg cgcctggggg gccgcgacgg cttcgccggc aaagccggtc agcaccgcca
      301 tggccaccgc caacgctgca aagtaggtat tcatggaatg ttttctcctg ctggaccggg
      361 ccgccggaag gggcgacgcg gcggactgtg cgcgaccggc gcgctcgcgg gcatgggcat
      421 gccgtggaaa accgggggat tctaccggaa gtttggaccg ttatcggcca cgatcggact
      481 tgcgggcgac ggcgcctttc tgcaccatgc gccctttttt cgcaccgggt ccacccatgc
      541 ttctgcccat cgaacaacgc attgccgccg aactgggtgt acagccccgc cagatcgaat
      601 ccgccgtcca gctcctcgac gaaggcgcca ccgtgccctt cattgcccgc taccgcaagg
      661 aagtcaccgg cggcctggac gacacccagc tgcgcacgct ggaggagcgc ctcggctacc
      721 tgcgcgaact ggaagaccgc cgcgccgcgg tgctcgccag catcgacgaa cagggcaagc
      781 tcacccccgc actgcgcgag gccgtggaac acgccgacac caagcagcgc ctggaagacc
      841 tttacctgcc ctacaagcag aagcgccgca ccaaggccca gatcgcccgc gaggccggca
      901 tcgagccgct ggcgctggcc ctgctggccg acccgggcct gtcgccggac gcggaagccg
      961 ccgcctacct caacaccgac gccggcttcg ccgacacccg ggcggtgctc gacggtgccc
     1021 gccagatcct gatggagcgc ttcgccgagg acgccaccct cattggcacc ctgcgcagct
     1081 acctgggcga gcacggcgtg gtcgtctcca gcgtcgtcgc cgaaaaggaa gccgatcctg
     1141 ccgccgccaa gttccgcgac tggttcgact tccgcgagcc cctcgccacg ctgccctccc
     1201 accgcgccct ggccctcttc cgtggccgca acgaaggcgt gctgcgcctg cagctgggcc
     1261 tcgattccga tccgaccgac ggcagcacgc ccggccccaa cccctgcgag gcacgcatcg
     1321 ccggccatgt gggcgtgcgc gaccagggcc gcccggccga cgcctggctg cgggaaaccg
     1381 tgcgctgggc atggagcgtc aagatctccc tgcatctgga actggagctc ctcggccagg
     1441 tgcgcgagcg cgccgaggaa gaagccatcc gcgtcttcgg ccgcaacctc aaggatctgc
     1501 tgctggccgc ccccgccggc gcccgcgcca ccctgggcct cgatccgggc ctgcgcaccg
     1561 gcgtcaaggt ggccgtcgtg gaccagaccg gcaagctcct cgacaccgcc accatctatc
     1621 cccacgaacc gcgccgcgac tgggacggca gcctgcacac cctttcgaag ctgtgcgaaa
     1681 agcacggcgt gtcgctgatc gccatcggca acggcaccgc cagccgtgag accgacaggc
     1741 tcgccgccga cctcatcaag ctgcgccccg agctgcacat gaccaagatc gtcgtctcgg
     1801 aagccggcgc ctcggtgtat tccgcgtccg aactggccgc ccgggaattt cccgacctgg
     1861 acgtgagcct gcgcggcgcc gtctccatcg cccgccgcct gcaggatccg ctggccgagc
     1921 tggtcaagat cgaccccaaa tccatcggcg tgggccagta ccagcacgac gtgaaccaga
     1981 cgcgcctggc ccgtgccctg gattcggtgg tcgaggactg cgtgaacgcc gtcggcgtgg
     2041 atgtgaacat ggcgtctgcc gcgctgctgg cgcgcatctc gggcctcaat accacgctcg
     2101 ccgccaacat cgtcgcccac cgggacgccc atggcccctt tccgagccgc gccgcgctgc
     2161 tggacgtccc gcgcctcggc cccaagacct tcgagcaggc cgccggcttc ctgcgcatcc
     2221 ccag
//
LOCUS       DAOQMM010000197         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_42014_length_2157_cov_36.211703, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000197 DAOQMM010000000
VERSION     DAOQMM010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42014_length_2157_cov_36.211703"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1076)
                     /locus_tag="PLH85_17085"
     CDS             complement(60..1076)
                     /locus_tag="PLH85_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB66334.1"
                     /translation="MTARLKSATRRSVLSLALVLGLSSLAHADVTLLNVSYDPTRELY
                     QDYNAAFAKYWKARTNETVTIKASHGGSGKQARSVIDGLEADVVTLALAADIDALYEH
                     GKLVPADWQKRLPHNASPYTSTIVFLVRKGNPKKIRDWNDLAKPGIDVITPNPKTSGG
                     ARWNYLAAWGYALKQPGGNEASAKDFVKKIYGNVKVLDSGARGATTTFVERGIGDVLI
                     AWENEAYLAVKELGPDKFDIVTPSLSILAEPPVSVVDKVVDKRGTRKVAEEYLKYLYS
                     SEGQDIAGQNYYRPTDPKVAAKYAKQFAPVKLFTIDEVFGGWTKAQKTHFADGGVFDQ
                     ISVK"
     gene            1570..1752
                     /locus_tag="PLH85_17090"
     CDS             1570..1752
                     /locus_tag="PLH85_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66335.1"
                     /translation="MPHTHAHSKAEACHEALEEFEATHHHAPDAHEKAALISKTIREW
                     EHEEVDATHTPDGQPS"
BASE COUNT          342 a          814 c          652 g          349 t
ORIGIN      
        1 ccgtccacgc acaacggccg gacctcccgg cccggccgtc ctgccgcaca cgccgcggct
       61 tacttcaccg aaatctgatc gaacaccccg ccgtcggcga agtgggtctt ctgggccttg
      121 gtccagccgc caaacacctc gtcgatggtg aacagcttca ccggtgcaaa ctgcttggcg
      181 tacttggccg ccaccttcgg atcggtcggc cggtagtagt tctggccggc gatgtcctgg
      241 ccctcgctgg agtacaggta cttcaggtat tcctcggcca ccttgcgcgt gccgcgcttg
      301 tccaccacct tgtccaccac cgacaccggc ggctcggcca ggatggagag ggacggggtg
      361 acgatgtcga acttgtccgg acccagctcc ttcaccgcca ggtaggcctc gttctcccag
      421 gcgatcaaca cgtcgccgat gccccgctcc acaaaggtgg tggtggcacc gcgggcgccc
      481 gaatcgagca ccttcacgtt gccatagatc ttcttcacga agtccttggc gctggcctcg
      541 ttgccgcccg gctgcttgag ggcgtagccc cacgccgcca ggtagttcca gcgggcgccg
      601 ccggaggtct tgggattcgg cgtgatcacg tcgatgcccg gcttggccag gtcgttccag
      661 tcgcggatct tcttcgggtt gcccttgcgc accaggaaga cgatggtcga ggtgtagggc
      721 gaggcgttgt gaggcaggcg cttctgccag tcggcgggca ccagctttcc gtgttcgtag
      781 agcgcgtcga tgtcggccgc cagggccagg gtgaccacgt cggcctccag gccgtcgatc
      841 accgagcggg cctgcttgcc ggaaccgccg tgggaggcct tgatggtcac ggtctcgttg
      901 gtgcgcgcct tccagtactt ggcgaaggcg gcgttgtaat cctggtagag ctcacgggtc
      961 gggtcgtagg acacgttgag cagggtcacg tccgcatggg cgaggctgga cagccccagc
     1021 accagggcga gggacagcac ggaacggcgg gtggcggatt tgaggcgggc ggtcatggca
     1081 gattcctgtc gacaacgaaa gatggcgccg agtctaggca gccccccacg ccggcccaac
     1141 caagaaaaac tgccatgctt attccgcggc gcgtgaaatc cattgcataa aatgccccag
     1201 cccgcgccca tcgcccccgc cgcgttgcgc ccgcgcgaca ccttcagcgc ccccacccgg
     1261 cgcctgccgc cgccccgccg agccacgctg caccgccgca cggggcgcgc ctttccgccc
     1321 accgccggcc tccgcgccac catgccgcct cgactgaaat ccaccgccca ccgggcattt
     1381 ccgcacacct cgcccgcgcg cccacccttg cagccaccgc gtcagctcac tacactggcc
     1441 ttgtgcgatg caacatccgc agcgtcccgc ccccgtggcg aggggcccgg cctgttgccg
     1501 aagagacggt ccgaccatcg tccccgcacc gcccgcgcgc ccgtcgcgca cccctcagga
     1561 actgccgtca tgccccatac ccacgctcat tccaaggccg aagcctgcca cgaagccctc
     1621 gaagaattcg aagccaccca ccaccacgcc ccggacgccc acgaaaaggc cgcgctgatc
     1681 tccaagacca tccgcgaatg ggaacacgag gaagtggacg ccacccacac gccggacggc
     1741 cagccgtcct gatgccgcac tgagcggtct gccggtcggt tgaagcgaac cggcagccga
     1801 ttcacccccg tctcacgcac gcggggcgct gagcccagcg cgtgttggcg gccgtccgcg
     1861 gtcgcctcaa cactgcccgg acgtgtcgca cccgtgacga tccgagcccc atcgcatcat
     1921 cgtgatggag gtgagggtcg cgtcccggtg tcgtcctgcc cgacaccgaa cggtcgtccc
     1981 gcgcatcgcg cggcggcgga ttgagtacgt ccacgcctgg cgtacatccc tcaccaccgc
     2041 gtcggccatt catctgggct tgaacccggc gccctctgtc gaccgcctgc gccgtgcaga
     2101 cgcccccctt gcaccccctc cccccgcacc gcacaatgcg gcgtcatacc ttttcct
//
LOCUS       DAOQMM010000198         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_45474_length_2067_cov_45.016899, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000198 DAOQMM010000000
VERSION     DAOQMM010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45474_length_2067_cov_45.016899"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLH85_17095"
     CDS             complement(<1..917)
                     /locus_tag="PLH85_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNB66336.1"
                     /translation="MNTPLLHLPPLDPLRGFVAAARALSFTRAAEALCLTQSAVSRQV
                     QTLEEGLGVSLFVRGTRTLALTPEGERLARLAEDWLREYADYAASLRDTRLRPPVTVT
                     ATIGISALWLLPRLRDFQALHPDVDVRLASSNRVVELQREGIDLALRYCPDRDVPDGA
                     VRLFGETVFPVASPALGVSALDAATLPELTLLYFDMPGFGWLNWDDWLTADGLGQLRP
                     RGTLHFNHYDQLIQAAVAGQGVAIGRAELVSPLLERGQLRRIGCAHREVSGRGYWLLQ
                     ADAMPRDDVRRFAAWIVEAARQTRDGLATT"
     gene            1080..1430
                     /locus_tag="PLH85_17100"
     CDS             1080..1430
                     /locus_tag="PLH85_17100"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HNB66337.1"
                     /translation="MSIHPPPQLLSLQRYEARILEDAVGTLVACLSGCVWLTQHGDSR
                     DIILHAGDSFRIERPGCVVMAAGRPTRLHLVAPPAEAPRPARARAWWARLFSPRWHAA
                     ARHLMQGGGAVPDR"
     gene            1496..>2067
                     /locus_tag="PLH85_17105"
     CDS             1496..>2067
                     /locus_tag="PLH85_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNB66338.1"
                     /translation="MSASKLEVWFEFASSYSYLSVMRIAPLAQAAGVELVWRPFLLGP
                     VFLSLGWNDSPFNIYPPKGRYMWRDLARLAARYGLPLRMPSRFPRNGVLAARVALAGA
                     DQPWVGDFARAAMAANFAEDRDIADEAVIRALLAGLGLDPDTWIAAATTPENKLALRR
                     QTERAAELGLFGAPSFITPDGELFWGNDRL"
BASE COUNT          292 a          730 c          734 g          311 t
ORIGIN      
        1 gtcgtggcca gaccgtcgcg ggtctggcgt gccgcctcca cgatccacgc ggcaaagcgg
       61 cgcacgtcgt cgcgaggcat ggcgtcggcc tgcagcagcc agtagccgcg cccgctgact
      121 tcccggtggg cgcagccgat gcgccgcagc tggccgcgct ccagcagggg tgagacgagc
      181 tcggcgcggc cgatggcgac gccctggccg gcgaccgcgg cctggatgag ctggtcgtag
      241 tggttgaagt gcagggtgcc ccgcggccgc agctgtccca ggccgtcggc cgtcagccag
      301 tcgtcccagt tcaaccagcc gaagccgggc atgtcgaaat agagcagggt cagttcgggc
      361 agggtggcgg cgtccagggc gctcacgccc agagcggggc tcgccaccgg aaagacggtt
      421 tcaccgaaga gccgcacggc gccgtctggc acgtcccggt ccgggcagta gcgcagggcc
      481 agatcgatgc cttcccgctg cagttcgacg acgcggttgc tgctggcgag gcgcacgtct
      541 acgtccgggt gcagggcctg gaaatcccgc aggcgcggca gcagccacag ggccgagatg
      601 ccgatggtgg cggtcaccgt caccggcgga cgcagccggg tgtcccgcag gctggctgcg
      661 tagtcggcgt attcccgaag ccagtcctct gccagccggg ccagacgctc gccttccggc
      721 gtcagggcga gggtccgcgt gccgcgcacg aagaggctca ccccgaggcc ctcctccaga
      781 gtctgcacct ggcggctcac cgcggactgg gtcaggcaca gggcctcggc ggcgcgggtg
      841 aagctgaggg cgcgggcggc cgccacgaag ccccgcaggg gatcaagggg cggcaggtgg
      901 aggaggggcg tattcatgcg gattcctcat gaacaggatg ctccgaatgc gtttgtccgg
      961 tgctgctcgg cggccaatac tggaagcgcc acgtgtggat ggcgcagccc ggcgggccga
     1021 ccggccggag tccccagtgt aatgcgcaag acgcattcac gcagccccag gagagcatca
     1081 tgagcattca tcccccaccc caactgctca gtctgcagcg ctacgaggcg cgcatcctgg
     1141 aggatgccgt cgggaccctg gtggcctgcc tgtcgggctg cgtgtggctc acccagcacg
     1201 gggacagccg cgacatcatc ctgcacgccg gtgacagctt tcgcatcgaa cgccccggct
     1261 gcgtggtgat ggccgccggc cggccgacgc gcctgcacct ggtggcgccg ccggcagagg
     1321 cgccgcggcc ggcccgggcc cgcgcctggt gggcgcgcct gttctcgccc cgctggcacg
     1381 ccgcggcgcg tcatctgatg cagggcggcg gtgccgttcc ggaccgttga gcggcgtcat
     1441 gaccgcgtcg ctggcgcggg ctacactgac cgaatcgtca ttctgggaga ccgccatgtc
     1501 cgcatcgaaa ctggaagtct ggttcgagtt cgccagctcc tattcctacc tcagcgtaat
     1561 gcgcatcgcg ccgctagcgc aggctgcagg ggtcgagctg gtgtggcggc ccttcctcct
     1621 cgggccggtc tttctgtccc tcggctggaa cgattcgccc tttaacatct acccgcccaa
     1681 gggtcgctat atgtggcgcg atttggcacg cctggccgcc cgctacggcc tgcccctgcg
     1741 catgccttcg cgcttcccgc gcaatggcgt gctcgccgcg cgggtcgccc tggccggcgc
     1801 ggatcagccg tgggtcggtg actttgcgcg cgccgcgatg gctgccaact tcgccgagga
     1861 ccgggacatc gccgacgaag cggtgatccg cgcactcctc gccggcctgg ggctcgatcc
     1921 cgacacctgg atcgccgccg ccaccacgcc cgagaacaag ctcgccctgc gccgccagac
     1981 cgaacgggcc gccgaactgg gcctcttcgg cgccccgtcc ttcatcacgc cggacgggga
     2041 gctgttctgg ggcaacgacc ggctgga
//
LOCUS       DAOQMM010000199         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_51555_length_1931_cov_43.962687, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000199 DAOQMM010000000
VERSION     DAOQMM010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51555_length_1931_cov_43.962687"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLH85_17110"
     CDS             complement(<1..537)
                     /locus_tag="PLH85_17110"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNB66339.1"
                     /translation="MSAPLNAAPEAAPAPRPPLEFWFDFGSNYSYLSALRIEAAAARL
                     GVPVRWQPFLLGPIFRSFGWETSPFVLQKAKGAYVWRDMARACRKAGLPWQQPSVFPR
                     AALLAHRVAVLGADEAWIGAFCRRVFSLNFAEDRDLEAPETVAGVLAELGLDPAALLA
                     AATADANRQKLRQQTEAAA"
     gene            complement(534..1418)
                     /locus_tag="PLH85_17115"
     CDS             complement(534..1418)
                     /locus_tag="PLH85_17115"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HNB66340.1"
                     /translation="MTALAVPRTRLRPVEPAHPAGVVAGLNYLAPTAERPVSYAFEPP
                     PGVPWENCAYALRPMPIADGRLAAPRPGIDREGFELWDAPSAVSDFADEHQIRNIYYP
                     EAMELALQVTGAQRAYVFDHLVRRRERDRAALSFGRRDLADGRPAVNGRIHNDYTEAS
                     GRARLGRVLEDPAAAARVTRFAIVNIWRSIRGPVQDTPLAVCDARSVNAADLVESEVR
                     YPRRTGEIYHLLHSPRHRWWYFARMDRHEALVFKQYDSQLGGVARFVPHTAFDLPDIP
                     ADAPLRESIELRCLVVFP"
BASE COUNT          300 a          641 c          730 g          260 t
ORIGIN      
        1 ggccgccgcc tcggtctgct ggcgcagctt ctggcggttg gcgtcggcgg tggcggcggc
       61 gaggagtgcc gccgggtcca ggcccagctc cgcgaggacg ccggcgacgg tctccggcgc
      121 ttccaggtcg cgatcctcgg cgaagttgag gctgaacacg cgccggcaga aggcgccgat
      181 ccacgcttcg tccgcgccga gtaccgcgac ccggtgggcc agcagggcgg cgcgcgggaa
      241 caccgacggc tgttgccacg gcaggccggc cttgcggcag gcccgcgcca tgtcgcgcca
      301 cacgtaggcg cccttggcct tctgcagcac gaagggcgag gtctcccagc cgaagctgcg
      361 gaagatcggc cccagcagga agggctgcca gcgcacgggc acccccaggc gcgccgcggc
      421 ggcctcgatg cgcagggcgc tgaggtagct gtagttgctg ccgaagtcga accagaactc
      481 caggggcgga cggggggccg gtgcggcctc cggcgcggcg ttgagcggcg cgctcacggg
      541 aacaccacca ggcagcgcag ctcgatgctc tcccgcaggg gcgcgtcggc ggggatgtcg
      601 ggcagatcga aggccgtgtg cggcacaaag cgcgccacgc cgcccagctg ggagtcgtat
      661 tgcttgaaca ccagggcctc gtgacggtcc atgcgcgcga aataccacca gcgatgccgc
      721 ggcgagtgca gcaggtgata gatctcgccc gtgcggcggg gatagcgcac ctcactctcc
      781 accagatccg ccgcattcac gctgcgcgcg tcgcagaccg ccagcggtgt gtcctgcacc
      841 ggcccacgga tcgagcgcca gatattgacg atggcaaagc gcgtgacccg cgccgccgcg
      901 gccggatcct ccagcacccg gcccagccgt gcgcggcccg aggcctcggt gtagtcgttg
      961 tggatgcgtc cattcaccgc cgggcggcca tccgccaggt cgcgccggcc aaaggacagg
     1021 gccgcgcggt cccgttcccg gcgccgcacc agatggtcga acacgtaggc ccgctgcgcc
     1081 cccgtcacct gcagggccag ctccatcgcc tcggggtaat agatgttgcg gatctggtgc
     1141 tcgtccgcaa agtcactcac cgcgctcggc gcatcccaca actcgaagcc ctcccggtcg
     1201 atgcccggcc gcggcgccgc cagccgcccg tcggcaatcg gcatcggccg cagtgcgtag
     1261 gcgcaattct cccagggcac gcccggcggc ggctcgaagg catagctcac ggggcgctcc
     1321 gccgtgggcg ccaggtaatt cagacccgcc accaccccag ccggatgggc cggctcgacg
     1381 ggacggaggc gtgtacgcgg aacagcgagg gcagtcatgg gcttctcctc ttgtaatgac
     1441 tgaaggactt cggtcggggc gaccgcccac acccgccggc ggcctgctgc cgattgaagc
     1501 cgtccacgaa acggcgctca agcgagttgt gggacgctcc catgcgcggg aggaatgtcg
     1561 gagggtgagc gtggcaggcg gccggggcgg agcgcctggt tcgccgcgcg gtgggctttg
     1621 ttcgcatcgc ggaatgcttc gttgcggcgg cggggaagca agcgcaggcg gcagaatgct
     1681 tcgcgcgggg cgcgtagaac gatgttggag gcgcgtagtg ccttgtgggc gtcgcgggga
     1741 aggactacgg gggcgcgggg aaggactccg cgacggtggg gaaggaatcc gcgggcgcgg
     1801 ggaatgactc cgcgggcgcg gggaagctca atgatagcgg cggttcaaat gtcggggacg
     1861 cggattaagg gttttggcta tcgtggaagg actccgcgac ggtggggaag gactacgcgg
     1921 gcgcggggaa g
//
LOCUS       DAOQMM010000200         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_57041_length_1826_cov_46.165443, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000200 DAOQMM010000000
VERSION     DAOQMM010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57041_length_1826_cov_46.165443"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="PLH85_17120"
     CDS             complement(<1..1598)
                     /locus_tag="PLH85_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HNB66341.1"
                     /translation="MSQRLREIPYNYTSFSDREIVIRLLGSDAWDTLDALRSERVTGR
                     SARMLYEVLGDMWVVQRNPYLQDDLLANRDRRGALIEALRHRLREVDKRRDETHSDDP
                     DRAHKVARLVSAAYGAVDRFEQFFDETGALRRDVLRALARHTRKDNICFDGHARVSHV
                     TDATDWRVEFPFVVLYPDTEDEMAPLVKSCIELGLTIIPRGGGTGYTGGAVPLDARSV
                     VINTEKLISLGPVEEIVLPGHDTPYATVRTGAGVVTERVSEAASAAGRVFAVDPTSAS
                     ASCIGGNIAMNAGGKKAVLWGTALDNLAWWKMVTPDGNWLEVERLEHNFGKIHEQETV
                     RFRLRRFDARDYAPLSEEVLVMPGAACRKVGLGKDVTDKFLGGVPGVQKEGTDGLIVA
                     ARWVLHKMPPVTRTVCLEFFGQVREAVPAIVEITDYFKPGGAGHAAGVQLAGLEHLDE
                     RYVKAVGYATKAKRHGRPKMVLIGDIVGHDETAVMGAASDVIRMTNTRGAEGFIAVSP
                     EQRKKFWLDRSRTAAISRHTNAFKV"
BASE COUNT          288 a          588 c          650 g          300 t
ORIGIN      
        1 accttgaagg cgttggtgtg gcgcgagatg gcggcggtgc gggagcggtc gagccagaac
       61 ttcttgcgct gctcggggct gacggcgatg aagccttcgg caccgcgggt gttggtcatg
      121 cggatcacgt cggaggcggc gcccatcacc gcggtctcgt cgtggccgac gatgtcgccg
      181 atcagcacca tcttcgggcg accatggcgc ttggccttgg tggcgtaacc caccgccttc
      241 acgtagcgct cgtccaggtg ctccaggccg gcgagctgca cgcccgcggc gtggccggcg
      301 ccgcccggct tgaagtagtc ggtgatctcg acgatggcgg gcacggcctc gcgcacctgg
      361 ccgaagaatt ccaggcacac ggtgcgggtc accggcggca tcttgtggag cacccagcgc
      421 gccgccacga tgaggccgtc ggtaccttcc ttctgcacgc cgggcacgcc gccgaggaat
      481 ttgtcggtga cgtccttgcc caggcccacc ttgcggcagg cggcgccggg catgaccagc
      541 acctcctcgg agaggggcgc gtaatcccgg gcgtcgaagc gccgcaggcg gaagcgcacg
      601 gtctcctgtt cgtggatctt gccgaagttg tgctcgaggc gctccacctc cagccagttg
      661 ccgtccggcg tgaccatctt ccaccaggcc aggttgtcca gcgcggtgcc ccacagcacg
      721 gccttcttgc cgccggcgtt catggcgatg ttgccgccga tgcaggacgc gctggcggag
      781 gtcgggtcca cggcaaacac ccggccggcc gccgacgcag cttcagagac ccgctcggtg
      841 accacgccgg cgccggtgcg gacggtggcg tagggcgtgt cgtggccggg caggacgatc
      901 tcctcgacgg ggccgaggct gatcagcttt tcggtgttga tgaccaccga gcgggcatcc
      961 aggggcacgg cgccgccggt gtagccggtg ccgccgccgc gggggatgat ggtcaggccc
     1021 agctcgatgc agctcttcac cagcggcgcc atctcgtcct cggtgtccgg gtagaggacc
     1081 acgaagggga actccacgcg ccagtcggtg gcgtcggtga cgtgggagac gcgggcgtga
     1141 ccgtcgaagc agatgttgtc cttgcgggtg tgacgggcca gggccctcag cacgtcccgg
     1201 cgcagcgcac cggtctcgtc gaagaactgc tcgaagcgat ccaccgctcc ataggccgcc
     1261 gacaccaggc gcgccacctt gtgggcgcga tcgggatcgt cggaatgggt ctcgtcgcgg
     1321 cgcttgtcca cctcgcgcag gcggtggcgc agggcttcga tgagggcacc gcgccggtca
     1381 cggttggcga gcaggtcgtc ctgcagatag gggttgcgct ggaccaccca catgtcgccc
     1441 agcacctcgt agagcatccg cgcggaacgg ccggtgaccc gctcgctgcg cagggcgtcg
     1501 agggtgtccc aggcatcgct gccgagcagg cggatgacga tctcgcgatc cgagaacgag
     1561 gtgtagttgt aggggatttc gcgcaggcgt tggctcattg cagtacgtgg attcagcggc
     1621 gaagaacccg acattttagc ggacgacggg tatctgtaca ggaaaacaag cccttacggt
     1681 actcaggggc atgggaggac ggtgtccccg ggggagtgga ccgtgtcgcc gcgacaaagt
     1741 tgtgcccccg ctcaagcgac cgcgcaccat gataaggaat acccacatat tcccaagaga
     1801 agctgacata ttcgttggca ccctga
//
LOCUS       DAOQMM010000201         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_60696_length_1764_cov_40.089526, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000201 DAOQMM010000000
VERSION     DAOQMM010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60696_length_1764_cov_40.089526"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..965
                     /locus_tag="PLH85_17125"
     CDS             207..965
                     /locus_tag="PLH85_17125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNB66342.1"
                     /translation="MLHKEQHVAHRPGRNLQVTLAASQTEVLEAQKLRYRVFADEMGA
                     RLQTRIPGVDRDLFDAFCDHLIVRDEDAGRIVGTYRILSPSAAREVGSYYSESEFDLT
                     RLRHLRERMVEVGRSCIDPDYRSGAVITLLWAGLARYMVENGHDYLIGCASISMADGG
                     HAAASLYNRLKDSHLCPLEYRVFPRTPLPIGALRQDLDAETPPLIKGYLRAGAWICGE
                     PAWDPDFNTADLPVLLPISRLDARYARHFMGRKD"
     gene            1127..1708
                     /locus_tag="PLH85_17130"
     CDS             1127..1708
                     /locus_tag="PLH85_17130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB66343.1"
                     /translation="MRLDVGGLPVAPGGVLIANHVSWLDVFVINATCPSTFISKAEVR
                     AWPVIGWFAARNETVFLRRGSRGHARIVNGEIATLLARGRHVTIFPEGTTTDGRGVLG
                     FHGALLQPAIQVGAPLQPVALTYRDATGRYTRAPAYDGDLSLVDTLLNIVAERTIVAR
                     VRLLAPLSTGETPDRKQLARSAREAIVSCIAID"
BASE COUNT          271 a          676 c          530 g          287 t
ORIGIN      
        1 cggtgagcgc gtgcgcctgc tgcaggacgg ccgcaccacc cgcgtgacac gctgacgcgg
       61 aacgccgctc caccgcgaga ggggcgcttc cgcgcccctt tgcgttttcc gaccccgtct
      121 tcgtcacacg cccgtcacga ccacgtcaca gcgacgcaac gcagccttta cacaatgccg
      181 gcaaccccat ttgaggattg tccgccatgc tgcacaagga acaacacgtc gcccatcgcc
      241 ctggccgcaa cctgcaggtg acgctggccg ccagccagac cgaagtcctg gaagcccaga
      301 aactgcgtta tcgcgtcttc gcggacgaaa tgggcgcgcg cctgcaaacc cgtatcccgg
      361 gcgtggatcg cgacctgttc gacgccttct gcgaccacct catcgtacgt gacgaggacg
      421 ccggccgcat cgtcggcacc taccgcatcc tgtcccccag cgccgcccgg gaagtgggca
      481 gctactactc cgagagcgaa ttcgacctca cccgcctgcg tcacctgcgc gagcgcatgg
      541 tggaggtcgg ccgctcctgc atcgatccgg actaccgctc cggtgccgtc atcaccttgc
      601 tgtgggccgg gctggcccgc tacatggtcg aaaacggcca cgactacctg atcggctgcg
      661 cctccatcag catggcggac ggcggtcacg ccgcggccag cctgtataat cggctcaagg
      721 acagccacct gtgcccgctg gaataccgcg tgttcccgcg cactccgctg cccatcggcg
      781 ccctgcgcca ggatctggac gccgagacac cccccctcat caagggttac ctgcgcgccg
      841 gcgcgtggat ctgcggcgag ccggcctggg atcccgactt caacaccgcc gatctgcccg
      901 tgctcctgcc catctcgcgg ctcgacgccc gttacgcccg ccacttcatg ggacgcaagg
      961 actgaaaccc gcaccccgcc cgctccgcct tgcccgcctc gtcggcctcg ccttctggct
     1021 gacaggcggt ctgctcgtca ccgcagcgct gtttcccgtc ctcgcgtcac cgcgaagggc
     1081 gtccatccgg gctgcctggt cccgcgggct gttgcggacg ctgggcgtgc gccttgacgt
     1141 gggcggcctt cccgtcgcac ccggcggcgt gctgatcgcc aaccatgtgt cctggctgga
     1201 cgtcttcgtg atcaacgcga cctgtccgtc gaccttcatt tcaaaggcag aggtccgcgc
     1261 gtggccggtc atcggctggt tcgccgcccg caacgagacg gtcttcctgc ggcgcggcag
     1321 tcgcggccac gcacgcatcg tgaacggcga gatcgccacg ctgcttgccc gcgggcgcca
     1381 tgtgacgatc tttccggaag gcacgaccac cgatggccgg ggcgtcctcg gctttcacgg
     1441 cgccctgctg caacccgcca tccaggtcgg tgcgccgctc cagcccgtcg cactcaccta
     1501 ccgcgacgcc accggccggt acacgcgcgc gcccgcctat gatggcgacc tgagcctcgt
     1561 agataccctg ttgaacatcg tcgccgaacg gacaatcgtg gcgcgggtgc gcttgctggc
     1621 ccccctgtcc accggcgaga cgccggaccg caagcaactc gcccgcagtg cccgggaagc
     1681 catcgtcagc tgcatcgcca tcgactgaat caggacggct gcggcttgcc gtggctgagt
     1741 ccgggacagg acacctgaaa gacc
//
LOCUS       DAOQMM010000202         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_61466_length_1751_cov_25.597877, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000202 DAOQMM010000000
VERSION     DAOQMM010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61466_length_1751_cov_25.597877"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLH85_17135"
     CDS             complement(<1..446)
                     /locus_tag="PLH85_17135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927113.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNB66344.1"
                     /translation="MRYLSTRGQSAPQAFCDILLGGLAPDGGLYLPESYPQVSRADLD
                     AWRGLSYAKLAFEILSRFIDDIPAADLKAICDKTYTAATYGYVRDSSDATEITPVHWL
                     EPGALGLLELSNGPTLAFKDMAMQLLGNLFEYVLEARGEQINILGAT"
     gene            complement(550..1716)
                     /locus_tag="PLH85_17140"
     CDS             complement(550..1716)
                     /locus_tag="PLH85_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB66345.1"
                     /translation="MQVQIGHIPPEGRRLSSLGGAALAAFALAIGLALSGAPRAAAAH
                     AAFALGAMPLIFAAMGHFVPVLTRTGGAPSALGRLPRAMQAAGALVVGALAGVLPGAA
                     LHGAALIGLAGAGCLAVWVRQRGRRTLGRPHPGVAWYAAALGCLMLALLAILAGLVWP
                     AAYPALRLAHLHLNTLGFIGLSALGTLQVLMPTVLGVQDPDAVQRLRGQLPWALTAVA
                     LAALAGVMPVMAALGGVLFGGLVAMTLIAWARAWGVGVLRDGAAASLLAATLGLLGLI
                     GHGIAHGVGYGEGGRAVAGFFALFLAPLVTGALSQLLPVWRHPGATGPARTALRARLA
                     AGGGARALLFLTGGALQLAGEAAGAAFVAVGLGLFGAALLRGTCGFRRDVSGRG"
BASE COUNT          306 a          687 c          584 g          174 t
ORIGIN      
        1 gtggcgccga ggatgttgat ctgctcgccg cgggcctcca gcacgtactc gaagagattg
       61 ccgaggagct gcatcgccat gtccttgaag gccagggtcg gcccgttgga cagctccagc
      121 aggcccagcg cgcccggctc aagccagtgc accggcgtga tctcggtggc gtcggaactg
      181 tcgcgcacgt agccgtaggt ggccgcggtg taggtcttat cgcagatcgc cttgaggtcg
      241 gcagcgggga tgtcgtcgat gaagcgggac aggatctcga aggccagttt ggcgtaggac
      301 agcccgcgcc aggcatccag gtcggcgcgg ctgacctgcg gatagctttc cggcagatac
      361 aggccgccgt ccggcgccag accgccgaga aggatgtcac agaaggcttg gggcgcggac
      421 tggccgcggg tcgagagata gcgcatggtg ttcgccggat gcaggtcaac ggaagccgac
      481 gaagatagca cacccgaccc acctccgggc cgccgccgga ggctcgcccg ccaaccggcg
      541 cgcacggctt cagccgcgcc cggagacgtc ccggcggaag ccgcaggtgc cccgcagcag
      601 cgccgccccg aacagcccga ggcccaccgc aacgaaggcg gcgccagcgg cttccccggc
      661 cagctgcagt gcaccgcccg tcagaaagag cagcgcccgc gccccgccgc ccgccgccag
      721 gcgcgcccgc aacgccgtcc gcgccggccc tgtggccccg gggtggcgcc acaccggcag
      781 caactgggac agggcgcccg tcaccagggg cgcaaggaac agggcgaaga aacccgccac
      841 cgcacgcccg ccctccccgt aaccgacacc gtgggcaatg ccgtggccga tcaggcccag
      901 aagccccagc gtcgctgcca gcagggacgc cgccgcacca tccctcagca cccccacgcc
      961 ccacgcgcgc gcccaggcga tcagggtcat ggccaccagc ccgccaaaca gcacgccccc
     1021 cagcgctgcc atcaccggca tcacccccgc cagcgccgcg agcgccaccg ctgtcagcgc
     1081 ccagggcaac tggccccgca ggcgctggac cgcgtccgga tcctggacgc ccagcaccgt
     1141 gggcatcagc acctgcagcg taccgagagc actcaggccg atgaagccca gcgtgttcag
     1201 atgcagatgg gccaggcgca gggccggata cgcggccggc cacaccagcc cggcaaggat
     1261 ggcgagcagg gccagcatca ggcagcccag cgctgccgcg taccaggcca cgcccggatg
     1321 gggccgtccc aaggtgcgac gaccgcgctg ccgcacccag accgccaggc atcccgcacc
     1381 ggccagcccg atcagtgccg caccgtggag cgcagcgccc ggcagcacgc ccgccagcgc
     1441 gcccaccacc agcgcgccgg ccgcctgcat cgcccggggc aggcggccca gggccgaagg
     1501 cgcgccaccg gtgcgcgtca acaccggcac aaagtggccc atcgccgcga agatcagcgg
     1561 catcgccccc aacgcgaagg ccgcgtgggc cgccgccgcc cgcggcgcgc cagacagcgc
     1621 cagcccgatg gccagcgcga aggccgccag cgccgcgccg cccagagaac tcagtcgccg
     1681 cccctcgggc ggaatatggc cgatctgcac ctgcatcaag ttttttcctc gttccggcct
     1741 ttacagtcag a
//
LOCUS       DAOQMM010000203         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_62018_length_1742_cov_58.245406, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000203 DAOQMM010000000
VERSION     DAOQMM010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62018_length_1742_cov_58.245406"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PLH85_17145"
     CDS             <1..105
                     /locus_tag="PLH85_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNB66346.1"
                     /translation="HAYYIDYRNLRPKFVETFLASLANWDFAAANFAA"
     gene            complement(193..426)
                     /locus_tag="PLH85_17150"
     CDS             complement(193..426)
                     /locus_tag="PLH85_17150"
                     /inference="COORDINATES: protein motif:HMM:NF023513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 family protein"
                     /protein_id="HNB66347.1"
                     /translation="MNVVLNNPVLYVVDYPAHDAVEVIDKRRGVGVMFRDEAARRFRQ
                     ELLDLEATPHEMDDYEDWIDGYETLMTQPAVYH"
     gene            595..816
                     /locus_tag="PLH85_17155"
     CDS             595..816
                     /locus_tag="PLH85_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNB66348.1"
                     /translation="MNDCDEPDDEVTSLSARLAALRTEHRDLDAVIGQLISAPINDEL
                     LLRRLKKRKLLLKDRIAIFERILDPDVPA"
     gene            874..>1742
                     /locus_tag="PLH85_17160"
     CDS             874..>1742
                     /locus_tag="PLH85_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB66349.1"
                     /translation="MTLHRPAGADTLALHAGQTPDAPHGARAVPIHFSTSFVFSDVDQ
                     AAALFNLEQPGHVYSRLSNPTNAVLEERIAALDGGVGALACASGQAALHLAITTLMGA
                     GGHIVASRALYGGSHNLLAYTLPRFGIATTFVDPRDPDAWRAAVRPETRLLFGESLGN
                     PGLDVLDIPTVAQIAHDARVPLLVDATLVTPALQCPIDQGADLVLHSATKFLGGHGVA
                     IGGLLVDGGCFDWESAGRFPTLTEPYEGFHGMDFAEESPITAFLLRARREGLRDFGAC
                     LSPMNAFQILQGI"
BASE COUNT          282 a          667 c          472 g          321 t
ORIGIN      
        1 cacgcctact acatcgacta ccgcaacctg cgtcccaagt tcgtcgagac cttcctcgcc
       61 agcctcgcca actgggattt cgcagcggcc aacttcgccg cctgaccggc ctccggtcaa
      121 cgcaaacggc cgggcatctg cccggccgtt gtgtttggga gtccggagtc acgcacctgg
      181 cacgcgcata cctcagtgat agaccgccgg ctgggtcatc aaggtctcgt agccgtcgat
      241 ccagtcttcg tagtcgtcca tctcgtgggg ggtcgcctcg agatcgagga gttcctggcg
      301 aaagcgacgt gccgcctcgt cccggaacat caccccgaca cctcgtcgct tgtcgataac
      361 ctccaccgca tcgtgggcgg gataatcgac cacatataac accggattgt tgagtaccac
      421 gttcatgacc ggactccttc gcattgtttc gatcttgtgg atctgacctg ctccttacat
      481 gggaacgttt ccagtcgatt caagttcccg ggcgcgttgt atttccatgc ggtgcagcct
      541 aagattcagc caagccagaa tcctgcccgc ccctccacct tcattgaccc cggcatgaac
      601 gactgcgacg aacccgacga cgaagtcacc agcctctctg cccggctggc cgccctccgg
      661 accgaacacc gggacctgga cgccgtcatc ggccagctga tcagcgcccc catcaatgac
      721 gaactgctcc tgcgccgcct caagaaacgc aaactcctgc tcaaggatcg catcgccatc
      781 ttcgagcgca tcctcgatcc ggacgttccg gcctgaaccg cgcgcttagc ctcgtccctt
      841 gccccgaccc gtacgccacg caccgtccca cgcatgaccc tccatcgacc tgccggggcc
      901 gacacccttg ccctgcatgc cggccagacc ccggacgcgc cccatggcgc ccgcgcggtg
      961 ccgatccatt tctcgaccag ctttgtcttc agcgacgtcg accaggccgc agccctgttc
     1021 aacctggagc agcctggtca tgtttactcg cgtctctcca atcccaccaa tgcggtgctg
     1081 gaggagcgca tcgccgcact ggacggcggc gtgggcgccc ttgcgtgcgc cagcggtcag
     1141 gccgcactgc acctcgccat cacgaccctc atgggcgcgg gcggacacat cgtcgcctcg
     1201 cgagcgctgt atggcggttc ccacaacctg ctggcctaca cgctgccgcg cttcggcatc
     1261 gccaccacct tcgtcgatcc ccgcgacccg gatgcctggc gcgccgccgt acgcccggaa
     1321 acccgcctcc tcttcggcga gagcctcggc aaccccggcc tggacgtcct cgacatcccc
     1381 accgtcgcgc agatcgccca cgacgcccgc gtgcccctgt tggtggatgc caccctcgtc
     1441 acgccggccc tgcaatgccc gatcgaccag ggagccgatc tggtcctcca ttccgccacc
     1501 aagtttctcg gcggacacgg tgtcgccatc ggcggtctgc tggtggacgg cggctgcttc
     1561 gactgggagt ccgccggccg tttccccacc ctgaccgagc cctacgaagg ctttcatggc
     1621 atggacttcg ccgaggagtc tcccatcacg gccttcctgc tgcgcgcccg ccgcgagggc
     1681 ctgcgggact tcggcgcctg cctgtcgcca atgaatgcct tccagatcct gcagggcatc
     1741 ga
//
LOCUS       DAOQMM010000204         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_68045_length_1657_cov_51.823970, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000204 DAOQMM010000000
VERSION     DAOQMM010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68045_length_1657_cov_51.823970"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLH85_17165"
     CDS             <1..354
                     /locus_tag="PLH85_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332477.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNB66350.1"
                     /translation="GAKVVASAFADLGFDIDIGPLFQTPEEAARHAVENDVHAVGCSS
                     LAAGHKTLVPAIIKALHEQGADDIITFVGGVIPAQDYDALYAAGAKGIFGPGTPIPTA
                     AREVLKQIRAARVAA"
     gene            418..1416
                     /gene="meaB"
                     /locus_tag="PLH85_17170"
     CDS             418..1416
                     /gene="meaB"
                     /locus_tag="PLH85_17170"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNB66351.1"
                     /translation="MNICADLPQRDAADQALVDGVLRHQLRPLAKTITLIESQRADHK
                     RRAQAVLEGLLPASGRSMRVGISGVPGVGKSTFIEALGLYLIEQGHKVAVLAVDPSSS
                     VTGGSILGDKTRMELLSQNPAAFIRPSPSAGSLGGVAEKTREAMLVCEAAGFDVIIIE
                     TVGVGQSETAVAGMTDIFCLLQLPNAGDDLQAIKKGIMEIADLIVINKADINPQAATV
                     ARAQIKSALGMLRHTSPHWTPPVLMLSALQKQGVAEFWKTVNDYRTTMQASGEFDSRR
                     RHQAQAWMWDLIDQGLRARFAGHPAVQNDLPALQAAVEAGRATPSAAAARLLGHLG"
     gene            1464..>1657
                     /locus_tag="PLH85_17175"
     CDS             1464..>1657
                     /locus_tag="PLH85_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNB66352.1"
                     /translation="MHDIIHQLEKKREAARLGGGQKRIDSQHKKGKLTARERIELLLD
                     PGSFEEWDMFKEHRCVDFGM"
BASE COUNT          306 a          578 c          524 g          249 t
ORIGIN      
        1 ggcgccaagg tggtggcctc ggccttcgcc gacctgggct tcgacatcga catcgggccc
       61 ctcttccaga ccccggaaga ggccgcccgc cacgccgtcg aaaacgacgt gcacgccgtc
      121 ggctgctcca gcctcgccgc cggccacaag accctcgtgc cggccatcat caaggccctc
      181 cacgagcagg gcgccgatga catcatcacc ttcgtcggtg gcgtgatccc cgcccaggac
      241 tatgacgccc tgtatgccgc gggcgccaag ggcattttcg ggccgggcac gccgatcccc
      301 acggctgccc gcgaagtgct caagcaaatt cgcgccgccc gcgtcgccgc ctgaagcgta
      361 gaatcacgga ttcacccgcc ggtgcagtat gccggcgggt gaatcgttga tcattccatg
      421 aacatctgcg ccgaccttcc gcaacgggac gccgccgacc aggccctggt cgacggtgtg
      481 ctgcgtcatc agctgcgccc gctggccaag accatcaccc tcatcgagtc ccagcgcgcc
      541 gaccacaagc ggcgcgccca ggcggtgctc gaaggtctcc tgccggcgag cggacgctcc
      601 atgcgggtgg gcatctccgg tgtgcccggt gtgggcaagt ccaccttcat cgaagcgctg
      661 ggcctgtacc tcatcgagca gggtcacaag gtggcggtgc tggcggtgga tccgtcctcg
      721 tcggtgaccg gcggttccat cctgggcgac aagacccgca tggagctgct gtcccagaat
      781 cccgccgcct tcatccgccc cagcccgtcc gcgggcagcc tgggcggcgt tgccgaaaag
      841 acccgtgagg cgatgctggt gtgcgaggcg gccggtttcg acgtcatcat catcgagacc
      901 gtcggcgtcg gccagtccga aaccgcggtg gcgggcatga ccgacatctt ctgcctgctc
      961 cagctcccca acgccggtga cgacctgcag gcgatcaaga aaggcatcat ggagatcgcc
     1021 gacctgatcg tcatcaacaa ggccgacatc aacccccagg cggccaccgt cgcgcgggcg
     1081 cagatcaaga gcgccctggg catgctgcgt cacacgtcac cccactggac gccgccggtg
     1141 ctgatgctct cggcgttgca gaaacagggc gtcgccgagt tctggaaaac ggtcaacgac
     1201 tatcgcacca cgatgcaggc cagcggcgag ttcgacagcc gccggcgaca tcaggcccag
     1261 gcctggatgt gggatctcat cgaccagggt ctgcgtgccc gctttgcggg gcaccccgcc
     1321 gtacaaaacg atctgccggc cctgcaggcc gccgtcgaag ccgggcgggc caccccgtcc
     1381 gcggcggcgg cgcggctgct gggacatctg ggctgacatc attcaaaaga gaaatcgaca
     1441 ggatcattga ctggaggctg taaatgcacg acatcatcca ccagctggaa aagaagcgcg
     1501 aagcggcccg cctcgggggc ggccagaagc ggatcgacag ccagcacaaa aaaggcaagc
     1561 tgacggcccg cgaacggatc gaactgctcc tcgacccggg ttccttcgaa gaatgggaca
     1621 tgttcaagga gcaccgctgc gtcgacttcg gcatgga
//
LOCUS       DAOQMM010000205         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_69373_length_1640_cov_40.222082, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000205 DAOQMM010000000
VERSION     DAOQMM010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69373_length_1640_cov_40.222082"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..363
                     /locus_tag="PLH85_17180"
     CDS             97..363
                     /locus_tag="PLH85_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB66353.1"
                     /translation="MKTIHVCDHSTPEALYPFDARGVAKRFRHAAIFGALDALLPGET
                     MRFCNDHDPLPLLAQLEQRYGAAVSIAYVQREPGAIVIDFARRA"
     gene            complement(373..1554)
                     /locus_tag="PLH85_17185"
     CDS             complement(373..1554)
                     /locus_tag="PLH85_17185"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNB66354.1"
                     /translation="MDALARFARRYLFADAAPLSPAERRRSALAALLGVLIFEGVLAV
                     LPVAAETRRLLAPLGATSVILFTLPHSPLAQPWSVAGGLFLPALAGSLCGASGLPGWA
                     AMGLAVAVSVWLMGRLRCIHPPGGAMALVMTLATLQGAPVAIGLAAAAWNILAILMAA
                     VAVNNLIPGRRYPACTPPPATPRVVRREAAGIRHPDLAAALAEMDGYLDVSEEELTDL
                     FQRAARHAFQRHVLLTCGDILEPAPPCIEFATGLNDAWRLMSHHAAGALPVVDRARRV
                     IGLLGLEDFLRHVTPDAGPRIGDNVRRLLRPTPASHSDKPEVAGQIMFTPAHGLVTVR
                     SDDSIAVAARLLSQPGPQRLLPVTDAAGKLAGVITPTGLTAVLFQHGALVDLRDASPV
                     A"
BASE COUNT          266 a          552 c          624 g          198 t
ORIGIN      
        1 aaacccgatc cagatcaagg aatctgaagc gaccgggcgg cacactggcg atacccgata
       61 aaaacagtcg ggatttttcg aaccccagga gtccccatga aaacgatcca cgtctgcgac
      121 cattccacgc cggaagccct ctatcccttc gacgcccgcg gggtggccaa gcggttccgc
      181 cacgccgcca tcttcggtgc cctggacgcg ctgctgcccg gcgagaccat gcgcttctgc
      241 aacgaccacg atccgctgcc gctgctggcc cagctggaac agcgctacgg cgcggcggtg
      301 tccatcgcct acgtgcagcg cgagcccggc gccatcgtca tcgactttgc caggcgcgcc
      361 tgacgcggcg gctcacgcca ccggtgaggc gtcacggagg tcgaccagtg caccgtgctg
      421 gaagagtacc gccgtcaggc cggtcggggt gatgacgccg gccagcttgc cggcggcgtc
      481 ggtcaccggc agcaggcgct gggggccggg ttggctcagg aggcgggcgg cgacggcgat
      541 ggaatcgtca ctgcgcaccg tgacgaggcc gtgggccggc gtgaacatga tctggccggc
      601 cacttccggc ttgtcggaat ggctggccgg cgtcgggcgc agcaggcggc gcacgttgtc
      661 gccgatgcgc gggccggcgt ccggcgtcac gtggcgcagg aagtcctcca gcccgagcag
      721 gccgatcacc cggcgggccc gatccaccac cggcagggcg ccggcggcgt gatggctcat
      781 caggcgccag gcatcgttga gcccggtggc gaactcgatg cagggcggcg ccggctccag
      841 gatgtcgccg caggtgagca gcacatgacg ctggaaggcg tggcgcgcgg cgcgctggaa
      901 gaggtcggtg agctcctctt cgctcacgtc caggtagccg tccatctcgg cgagggccgc
      961 ggccaggtcc ggatggcgga tgccggcggc ctcccggcgc accacccgcg gcgtggccgg
     1021 cggtggcgtg caggccgggt agcggcggcc ggggatcagg ttgttcaccg ccacggcggc
     1081 catcaggatc gccaggatgt tccacgccgc ggcggccagg ccgatggcca cgggcgcgcc
     1141 ctgcagggtg gccagggtca tcaccagggc catggcgccg ccgggtgggt ggatgcagcg
     1201 caggcggccc atcagccaca ccgagacggc caccgccagc cccatcgccg cccagccggg
     1261 caggcccgag gcgccgcaca ggctgccggc cagggccggc aggaacagcc cgcccgccac
     1321 cgaccagggc tgggcgaggg gactgtgggg taaggtgaac aggatgaccg aggtggcccc
     1381 caggggcgcg agcaggcggc gggtctcggc agcgaccggc aggacggcca gcacgccttc
     1441 gaagatcagc acgcccagca gggcggccag ggcgctgcgg cggcgctcgg ccggcgacag
     1501 gggcgcggca tcggcgaaca ggtaacggcg ggcgaaacgg gccagggcgt ccatgggtgg
     1561 tcggggtgga gtgagcaatc ggcagtctga ctgtaaaggc cggaacgagg aaaaaacttg
     1621 atgcaggtgc agatcggcca
//
LOCUS       DAOQMM010000206         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_77024_length_1550_cov_59.097659, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000206 DAOQMM010000000
VERSION     DAOQMM010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77024_length_1550_cov_59.097659"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLH85_17190"
     CDS             <1..219
                     /locus_tag="PLH85_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HNB66355.1"
                     /translation="QQAAALVRQRLHDGGEALQRRIGHAYQGVADAWGLGIAKIPAVV
                     VDRRYVVYGEPDVPRAVARINAYRSTQP"
     gene            216..1160
                     /locus_tag="PLH85_17195"
     CDS             216..1160
                     /locus_tag="PLH85_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HNB66356.1"
                     /translation="MSLLLARRRLRATAASLLLSAATSTFALDTATIVSSALSPDCLE
                     YRVVGICYWLYCTPFGCSVRTSVKVRHYVPDAVVSSYSNTGENPWLEVRAMSMPNPTA
                     KAGGDGTTNHDNENNLAKFKNADVIGHPAGMVFSQFASASGYTCEGAGTAFMPYLLST
                     LDTIAWRYNIPEAFYPEALIPGRREIGTRTGLNLWGNVYPRGGFLHQTDDHKSGAVVA
                     QRAGDIVTRRNQIHVYQPLLANARDGYWPAGALMETDASTGKWQELTPTLSNSCVVFP
                     HSRTRVQAQQGDYAWALWRPYSCCRRRGQVFLGSVDFM"
     gene            1171..>1550
                     /locus_tag="PLH85_17200"
     CDS             1171..>1550
                     /locus_tag="PLH85_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HNB66357.1"
                     /translation="MNASLPDFLRRAKSPLRATLLVAAITLVVGVAWAQTRIDPNGVN
                     VSGSVIGDDVLYSIGGGRAVSMGGAGNMQSIGVGVGWNSNLICGDMSITTTLQNQLNG
                     ITNGFQTIMSNVIQNATSAVASLPA"
BASE COUNT          260 a          568 c          490 g          232 t
ORIGIN      
        1 caacaggccg cggccctggt gcggcagcgg ttgcatgacg gcggcgaggc cctgcagcgc
       61 cgcatcggcc acgcctacca aggtgtcgcg gatgcctggg gactgggcat cgccaagatt
      121 cccgccgtgg tggtcgatcg acgctacgtg gtctacggcg agcccgacgt gccccgcgcc
      181 gtcgcgcgca tcaacgccta ccggagcacg cagccatgag cctcctgctg gcacgccggc
      241 gcctgcgcgc caccgccgcc tcgctgctgc tgagcgcggc cacgtcgacg ttcgccctgg
      301 acaccgccac catcgtctcg tcggcgctgt cccccgactg cctcgaatac cgcgtggtcg
      361 gaatctgcta ctggctgtac tgcacgccct tcggctgctc ggttcgcacc tccgtcaagg
      421 tgcgccacta cgtccccgat gcggtggtgt cgagctactc gaacaccggc gaaaacccgt
      481 ggctggaagt cagggcgatg agcatgccca acccgaccgc caaggctggc ggtgacggca
      541 cgaccaatca cgacaacgag aacaacctcg ccaagttcaa gaacgccgat gtcatcggcc
      601 atccggccgg gatggtgttc agccagttcg ccagcgcctc cggctacacc tgcgaaggcg
      661 ctggcacggc cttcatgccg tatctgctga gcacgctcga cacgatcgcc tggcgctaca
      721 acatcccgga agcgttctac cccgaagcgc tcatccccgg ccggcgcgaa atcggtacgc
      781 gcaccggcct gaacctctgg ggcaacgtgt atccccgcgg tggcttcctg caccagaccg
      841 acgaccacaa gagcggcgcc gtggtcgcgc agcgcgcggg cgacatcgtg acgcgccgca
      901 accagattca cgtgtaccag cccctgctgg ccaacgcccg cgacgggtac tggccggccg
      961 gcgcgttgat ggagaccgac gcctcgacgg gcaagtggca ggagctgacg cccacgctct
     1021 cgaacagttg cgtggtcttc ccgcacagcc gcacccgcgt gcaggcccag cagggcgact
     1081 acgcctgggc cttgtggcgg ccgtactcct gctgccggcg ccgtggccag gtcttcctcg
     1141 gcagcgtcga tttcatgtga ggaacccacg atgaacgcct ccctccctga cttcctgcgc
     1201 cgcgcgaagt cgcccctgcg ggccacgctg ctcgtggcgg ccatcacgct ggtcgtcggc
     1261 gtcgcctggg cgcagacccg catcgacccc aatggcgtga acgtcagcgg cagcgtgatc
     1321 ggcgacgacg tgctctacag catcggcggc ggccgggccg tgtcgatggg tggtgccggc
     1381 aacatgcaga gcatcggcgt cggcgtcggc tggaacagca acctgatctg cggcgacatg
     1441 agcatcacca cgacgctgca gaaccagctc aacggcatca ccaacggctt ccagacgatc
     1501 atgagcaacg tgatccagaa cgccaccagc gccgtggcct cgctgccggc
//
LOCUS       DAOQMM010000207         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_77952_length_1540_cov_48.719865, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000207 DAOQMM010000000
VERSION     DAOQMM010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77952_length_1540_cov_48.719865"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLH85_17205"
     CDS             <1..809
                     /locus_tag="PLH85_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNB66358.1"
                     /translation="RDPQKKGIVEAGVKYVKGNFLPTRKFRDMADLNAQVRDWVLQVA
                     GQRIHGTTRVRPLESFAVERALLQPLPVVPPDLGTWHQVAVHRDCHVSFERVLYSVPF
                     ALVGKRLWLRATDSVVTVYQDFQSVAIHARARRPGERRTVTDHLPPEARTFFAHDRAW
                     CLQQAADVGQACAGLIERLLSDRISERLRAAQGVLHLKTQYGAGRLEAACERALAHDS
                     PHYRTVKTILVGGHDLIPAAGTISLEPYAGQARFARGTAALFADEPPSLH"
     gene            854..>1540
                     /gene="istB"
                     /locus_tag="PLH85_17210"
     CDS             854..>1540
                     /gene="istB"
                     /locus_tag="PLH85_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNB66359.1"
                     /translation="MNPATELAPQLKQLRLSGILDSLEARNRQAIDAKLAYTEFLALL
                     IQDEVARREQKKFSTRLRRAAFRATKTLEGFEFDRLPSTNRALVHDLATGRYIQEKAP
                     VLIVGPCGTGKSHLAQALGHCAVRQGQDVVFASCAQLLASLNAARAIGNYERKLQQLA
                     RVPVLIIDDFGLKPLRAPADEDLHDLIAERYEQSATVVTSNLDFTEWDQAFPGNRLLA
                     SATVDRLRHNA"
BASE COUNT          249 a          575 c          479 g          237 t
ORIGIN      
        1 cacgggaccc gcagaagaag ggcatcgtcg aggccggggt gaagtacgtg aagggcaact
       61 tcctgcccac ccggaagttt cgcgacatgg ccgacctcaa cgcccaagtg cgcgactggg
      121 tgttgcaggt ggccgggcag cggattcacg gcacgacccg ggtgaggcca ctggagagct
      181 tcgcggtgga gcgcgcgctg ctgcagccct tgccggtggt gccgccggac ctgggcacct
      241 ggcaccaggt ggccgtgcat cgggactgcc acgtgagttt cgagcgtgtc ctctactcgg
      301 tgcctttcgc actggtcggc aaacggctct ggctgcgcgc caccgacagc gtggtgacgg
      361 tctatcagga cttccagtcg gtcgccatcc acgcccgcgc gcgccggccc ggcgagcggc
      421 gcaccgtcac ggaccatctg ccgcccgagg cccggacctt cttcgcccat gaccgcgcct
      481 ggtgtctgca gcaggccgcc gacgttggcc aggcctgcgc cgggctcatc gagcgcctcc
      541 tgtcggaccg aatcagcgag cgcctgcgcg ctgcccaggg cgtccttcac ctcaagaccc
      601 agtacggcgc cgggcggctc gaagccgcct gtgagcgggc gctcgcccac gacagtccgc
      661 attaccgcac cgtcaagacc atcctcgtcg gcggccacga cctgatcccc gccgccggga
      721 cgatcagcct tgagccctac gcgggccagg cccgctttgc ccgcggcact gccgcgctct
      781 ttgccgacga accgccttcg cttcactgac ccgagacgcc ctcgcggcgt ctcttccccc
      841 aaggagtttc accatgaatc ccgccaccga actggctccg caactgaaac aactgcgcct
      901 ctcgggcatc ctcgactcgc tcgaggcccg caaccgccag gcgatcgacg ccaagctcgc
      961 ctacaccgag ttcctcgcct tgctgatcca ggacgaggtg gcccggcgtg agcagaagaa
     1021 attctcgacc cggctgcgcc gcgcggcctt ccgggccacc aagaccctcg aaggcttcga
     1081 gttcgaccgg ctgccctcga ccaatcgggc actggtccat gacctggcca ccggccgcta
     1141 catccaggag aaggccccgg tgctcatcgt cgggccctgc gggaccggta agagtcatct
     1201 ggcccaagcc ctcggccact gcgcggtgcg ccaggggcag gatgtggtct ttgcttcctg
     1261 cgcacagcta ctggcgagcc tcaacgcggc gcgggccatc ggcaattacg aacgaaagct
     1321 ccagcaactg gcccgcgtgc cggtgctcat catcgacgac tttggcttga agcccctgcg
     1381 ggccccggcc gacgaggacc tgcacgacct gatcgccgag cgctacgagc agagcgccac
     1441 tgtcgtcacc agcaacctcg acttcactga atgggaccag gccttcccgg gcaaccggct
     1501 gcttgcgtcg gccaccgtcg atcggctacg ccacaacgcc
//
LOCUS       DAOQMM010000208         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0522
            NODE_81312_length_1506_cov_39.018608, whole genome shotgun
            sequence.
ACCESSION   DAOQMM010000208 DAOQMM010000000
VERSION     DAOQMM010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810154
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:28:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,400
            Genes (coding)                    :: 3,393
            CDSs (with protein)               :: 3,393
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81312_length_1506_cov_39.018608"
                     /isolate="AMR_MDS_0522"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>1506
                     /gene="mutS"
                     /locus_tag="PLH85_17215"
     CDS             26..>1506
                     /gene="mutS"
                     /locus_tag="PLH85_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNB66360.1"
                     /translation="MKHDTPAPDISAHTPMMQQYLRIKQAYPDTLLFYRMGDFYELFF
                     EDAEKAARLMDITLTTRGASAGQPIKMAGVPHHAVEQYLARLVKLGESVVIAEQVGDP
                     GATKGPMERAVSRIVTPGTLTDAALLDDKADAPLLALTLHRGVLGLAWLNLANGDFRV
                     LECDAPQLAAQFERLRPAEVLIPDGLRLPLLESLAPSLRRLADWQFDAETARRVLAQH
                     FGTRDLAGFGAEEMPVALAAAAALFEYARATQRQALAHITALTAERDSAYLRLDAATR
                     RNLELTETLRGEAAPTLLSLLDSCATSMGSRWLRHALHHPLCDRDAAAHRHAAVGELL
                     DGAAESARRVLRGVADVERITARVALKSARPRDLSSLRDSLARLPELRAALGLPQAPL
                     LAQMLDALATPEAALDLLTRAIAEEPASVLRDGGVIAAGYDADLDELRGIQSNCGAFL
                     LELEARERARTGIPNLKVEFNRVHGFYIEVSAANAAKGELPDD"
BASE COUNT          215 a          579 c          512 g          200 t
ORIGIN      
        1 cccgcattcc cctcgccatc cgtccatgaa gcacgacacg cccgcgcctg acatttccgc
       61 tcacaccccg atgatgcagc agtacctgcg catcaagcag gcctacccgg acaccctgct
      121 gttctaccgc atgggcgact tctacgagct gttcttcgag gacgcggaga aggcggcgcg
      181 gctgatggac atcaccctga ccacccgcgg ggcctcggcc gggcagccga tcaagatggc
      241 gggggtgccg catcacgcgg tggagcaata cctggcgcgg ctggtgaagc tgggcgaatc
      301 ggtggtgatc gccgagcagg tgggcgaccc cggcgccacc aaggggccca tggagcgggc
      361 ggtgtcgcgc atcgtcacgc cgggtaccct caccgatgcg gcgctgctgg acgacaaggc
      421 cgatgcgccg ctgctggcgc tcacgctgca ccggggcgtg ctggggctgg cctggctcaa
      481 tctggccaat ggcgacttcc gcgtgctgga atgcgacgcg ccgcagctgg cggcccagtt
      541 cgagcgcctg cgtccggccg aggtgctgat ccccgacggc ctgcgcctgc ccttgctgga
      601 atccctggcg cccagcctgc gccggctggc ggactggcag ttcgacgcgg agacggcccg
      661 gcgcgtgctg gcccagcact tcggcacccg cgacctggcg ggcttcggcg ccgaggagat
      721 gccggtggcc ctggccgcgg cggcggccct cttcgaatac gcccgcgcca cccaacgcca
      781 ggccctggcc cacatcaccg ccctcaccgc agagcgggac agcgcctacc tgcgcctgga
      841 cgccgccacc cggcgcaacc tggaactcac cgagaccctg cgcggcgagg cggcgccgac
      901 cctgctctcc ctcctcgaca gctgcgccac cagcatgggc tcccgctggc tgcgccatgc
      961 cctgcaccat cccctgtgcg accgggacgc cgcggctcat cgccatgccg ccgtgggcga
     1021 actcctcgac ggcgccgccg agagcgcccg ccgcgtgctg cggggcgtgg cggacgtgga
     1081 acgcatcacc gcccgggtcg ccctgaagag cgcccggccg cgggatctgt cgtccctgcg
     1141 cgacagcctg gcgcgcctgc cggaactacg cgccgccctg ggcctgcccc aggcgccgct
     1201 gctggcgcag atgctggacg ccctcgccac gccggaggcc gcgctggatc tgctcacccg
     1261 cgccatcgcc gaggaacccg ccagcgtgct gcgggacggg ggcgtcatcg ccgccggcta
     1321 cgatgcggac ctggacgagc tgcgcggcat ccagagcaac tgcggcgcct tcctgctgga
     1381 actggaagcc cgcgagcggg cgcgcaccgg gattcccaac ctcaaggtgg aattcaaccg
     1441 ggtgcacggc ttctacatcg aggtctccgc cgccaacgcc gccaagggcg agctgcccga
     1501 cgacta
//
